The Woods Hole Partnership Education Program (PEP) (Invited)

Science.gov (United States)

Jearld, A.; Liles, G.; Gutierrez, B.

2013-12-01

In March 2009, the Woods Hole Diversity Initiative (WHDI) launched the Partnership Education Program (PEP), a multi-institutional effort to increase diversity in the student population (and ultimately the work force) in the Woods Hole science community. PEP, a summer research internship program, is open to students of all backgrounds but is designed especially to provide opportunities for individuals from populations under-represented in science, technology, engineering, and mathematics (STEM) and who otherwise would not have had the opportunity to come to Woods Hole to study or do research. A month-long course, 'Ocean and Environmental Sciences: Global Climate Change,' sets the stage for their summer research projects. The PEP model is emerging as an effective and sustainable approach to bringing students into the STEM research community. PEP is carefully structured to provide critical support for students as they complete their undergraduate experience and prepare for careers and/or graduate school. In its first five years, PEP has brought to the Woods Hole science community more than 75 students from over 50 colleges and universities, including many that do not typically send talent into marine and/or ecological research. PEP is unusual (perhaps even unique) in that it is a collaborative initiative involving seven partner institutions. Working together, the PEP collaborative has established a critical mass of under-represented students who are now in graduate school and/or working in STEM areas.

PEP sextupoles

International Nuclear Information System (INIS)

Brunk, W.O.

1976-10-01

The sextupole requirements of PEP are such that they represent a much larger proportion of the ring magnet cost than they did in SPEAR. A study is given of various fabrication techniques all pointed toward cost reduction without an undue sacrifice of function quality. The point of debarkation is the sextupole design as described in the PEP Conceptual Design Report. There are three main areas of inquiry, namely, pole tip contour, coil material and fabrication and yoke size

The Danish PEP Registry

DEFF Research Database (Denmark)

Lunding, Suzanne; Katzenstein, Terese L; Kronborg, Gitte

2016-01-01

BACKGROUND: The risk of occupational exposures to blood cannot be eliminated completely and access to post-exposure prophylaxis (PEP) to prevent HIV transmission is important. However, PEP administration has been associated with frequent adverse effects, low compliance and difficulties to ensure ...

PEP-II: An asymmetric B factory

International Nuclear Information System (INIS)

1993-06-01

In this report, the authors have described an updated conceptual design for the high-luminosity Asymmetric B Factory (PEP-II) to be built in the PEP tunnel culmination of more than four years of effort aimed at the design and construction of an asymmetric e + e - collider capable of achieving a luminosity of L = 3 x 10 33 cm -2 s -1 . All aspects of the conceptual design were scrutinized in March 1991 by a DOE technical review committee chaired by Dr. L. Edward Temple. The design was deemed feasible and capable of achieving its physics goals. Furthermore, the cost estimate, schedule, and management plan for the project were fully endorsed by the committee. This updated conceptual design report captures the technical progress since the March 1991 review and reflects the lower cost estimate corresponding to the improved design. Although the PEP-II design has continued to evolve, no technical scope changes have been made that invalidate the conclusion of the DOE review. The configuration adopted utilizes two storage rings, an electron ring operating at 9 GeV and a positron ring at 3.1 GeV, each with a circumference of 2200 m. The high-energy ring is an upgrade of the PEP storage ring at SLAC; all PEP magnets and most power supplies will be reused. The upgrade consists primarily of replacing the PEP vacuum chamber and RF system with newly designed versions optimized for the high-current environment of PEP-II. The low-energy ring will be newly constructed and will be situated atop the high-energy ring in the PEP tunnel. Utilities already installed in the PEP tunnel are largely sufficient to operate the two PEP-II storage rings

Status Report on PEP-II Performance

International Nuclear Information System (INIS)

Matter, Regina S.

2002-01-01

PEP-II [1-9] is an e+e- collider with asymmetric energies (3.1 and 9 GeV, respectively) in a 2200 m tunnel at the Stanford Linear Accelerator Center. The collider produces B mesons to study a particle physics effect called CP violation as well as other physics topics. PEP-II was completed in 1998 with the first luminosity generated in July of that year. The installation of the BaBar Detector was finished in May 1999. The overall layout of PEP-II is shown in Figure 1 and the interaction region of PEP-II in Figure 2. The accelerator parameters and achievements of the High Energy Ring (HER) are listed in Table 1 and those for the Low Energy Ring (LER) in Table 2. The two beams collide at a single point in the IR2 hall where the BaBar detector is located. Beam parameters at the best luminosity are shown in Table 3 and PEP-II milestones in Table 4. In August 1999 PEP-II passed the world's record for luminosity which was 8.1x10 32 /cm 2 /s. The present luminosity in PEP-II is 2.15x10 33 /cm 2 /s which is 72% of the design. In June 2000 PEP-II delivered an integrated luminosity of 150 pb -1 in one day, which is above the design integrated luminosity per day of 135 pb -1 . Over the past year PEP-II has delivered over 12 fb -1 to BaBar. BaBar has logged over 11 pb -1 . The present plan is collide until the end of October 2000 followed by a three month installation period

PEP liquid level system

International Nuclear Information System (INIS)

Lauritzen, T.; Sah, R.C.

1981-03-01

A liquid level system has been installed in the accelerator housing of the PEP storage ring. This instrument spans the entire 2.2 km circumference of the PEP project, and over one hundred readouts provide reference elevations which are used for the accurate alignment of accelerator components. The liquid level has proven to be extremely precise (+-0.10 mm) and quick to use, and it has contributed to the accurate alignment of PEP before beam turn-on. Since the liquid level readouts are rigidly attached to the accelerator housing, the liquid level has been a convenient means to monitor the settling of the accelerator housing

Luminosity monitor at PEP

International Nuclear Information System (INIS)

Fox, J.D.; Franklin, M.E.B.

1981-02-01

The luminosity monitor system utilized by the MKII Detector and by the PEP operators is described. This system processes information from 56 photomultipliers and calculates independent luminosities for each of the 3 colliding bunches in PEP. Design considerations, measurement techniques, and sources of error in the luminosity measurement are discussed

PEP storage ring: current status

International Nuclear Information System (INIS)

Rees, J.R.

1981-02-01

This report comments on the performance of the subsystems of PEP, discusses the beam dynamical behavior of the machine and compares it with our expectations and, finally, describes plans for improving PEP

High-resolution spectrometer at PEP

International Nuclear Information System (INIS)

Weiss, J.M.; HRS Collaboration.

1982-01-01

A description is presented of the High Resolution Spectrometer experiment (PEP-12) now running at PEP. The advanced capabilities of the detector are demonstrated with first physics results expected in the coming months

Power Extension Package (PEP) system definition extension, orbital service module systems analysis study. Volume 9: PEP design, development and test plans

Science.gov (United States)

1979-01-01

A plan for the production of two PEP flight systems is defined. The task's milestones are described. Provisions for the development and assembly of new ground support equipment required for both testing and launch operations are included.

EFRT M-12 Issue Resolution: Comparison of Filter Performance at PEP and CUF Scale

Energy Technology Data Exchange (ETDEWEB)

Daniel, Richard C.; Billing, Justin M.; Bontha, Jagannadha R.; Brown, Christopher F.; Eslinger, Paul W.; Hanson, Brady D.; Huckaby, James L.; Karri, Naveen K.; Kimura, Marcia L.; Kurath, Dean E.; Minette, Michael J.

2009-08-13

Pacific Northwest National Laboratory (PNNL) has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to perform research and development activities to resolve technical issues identified for the Pretreatment Facility (PTF). The Pretreatment Engineering Platform (PEP) was designed and constructed and is to be operated as part of a plan to respond to issue M12, Undemonstrated Leaching Processes. The PEP is a 1/4.5-scale test platform designed to simulate the WTP pretreatment caustic leaching, oxidative leaching, ultrafiltration solids concentration, and slurry washing processes. The PEP replicates the WTP leaching processes using prototypic equipment and control strategies. The PEP also includes non-prototypic ancillary equipment to support the core processing. Two operating scenarios are currently being evaluated for the ultrafiltration process (UFP) and leaching operations. The first scenario has caustic leaching performed in the UFP-2 ultrafiltration feed vessels (i.e., vessel UFP-VSL-T02A in the PEP and vessels UFP-VSL-00002A and B in the WTP PTF). The second scenario has caustic leaching conducted in the UFP-1 ultrafiltration feed-preparation vessels (i.e., vessels UFP-VSL-T01A and B in the PEP; vessels UFP-VSL-00001A and B in the WTP PTF). In both scenarios, 19-M sodium hydroxide solution (NaOH, caustic) is added to the waste slurry in the vessels to leach solid aluminum compounds (e.g., gibbsite, boehmite). Caustic addition is followed by a heating step that uses direct injection of steam to accelerate the leach process. Following the caustic leach, the vessel contents are cooled using vessel cooling jackets and/or external heat exchangers. The main difference between the two scenarios is that for leaching in UFP1, the 19-M NaOH is added to un-concentrated waste slurry (3 to 8 wt% solids), while for leaching in UFP2, the slurry is concentrated to nominally 20 wt

EFRT M-12 Issue Resolution: Comparison of Filter Performance at PEP and CUF Scale

International Nuclear Information System (INIS)

Daniel, Richard C.; Billing, Justin M.; Bontha, Jagannadha R.; Brown, Christopher F.; Eslinger, Paul W.; Hanson, Brady D.; Huckaby, James L.; Karri, Naveen K.; Kimura, Marcia L.; Kurath, Dean E.; Minette, Michael J.

2009-01-01

Pacific Northwest National Laboratory (PNNL) has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to perform research and development activities to resolve technical issues identified for the Pretreatment Facility (PTF). The Pretreatment Engineering Platform (PEP) was designed and constructed and is to be operated as part of a plan to respond to issue M12, Undemonstrated Leaching Processes. The PEP is a 1/4.5-scale test platform designed to simulate the WTP pretreatment caustic leaching, oxidative leaching, ultrafiltration solids concentration, and slurry washing processes. The PEP replicates the WTP leaching processes using prototypic equipment and control strategies. The PEP also includes non-prototypic ancillary equipment to support the core processing. Two operating scenarios are currently being evaluated for the ultrafiltration process (UFP) and leaching operations. The first scenario has caustic leaching performed in the UFP-2 ultrafiltration feed vessels (i.e., vessel UFP-VSL-T02A in the PEP and vessels UFP-VSL-00002A and B in the WTP PTF). The second scenario has caustic leaching conducted in the UFP-1 ultrafiltration feed-preparation vessels (i.e., vessels UFP-VSL-T01A and B in the PEP; vessels UFP-VSL-00001A and B in the WTP PTF). In both scenarios, 19-M sodium hydroxide solution (NaOH, caustic) is added to the waste slurry in the vessels to leach solid aluminum compounds (e.g., gibbsite, boehmite). Caustic addition is followed by a heating step that uses direct injection of steam to accelerate the leach process. Following the caustic leach, the vessel contents are cooled using vessel cooling jackets and/or external heat exchangers. The main difference between the two scenarios is that for leaching in UFP1, the 19-M NaOH is added to un-concentrated waste slurry (3 to 8 wt% solids), while for leaching in UFP2, the slurry is concentrated to nominally 20 wt

InverPep: A database of invertebrate antimicrobial peptides.

Science.gov (United States)

Gómez, Esteban A; Giraldo, Paula; Orduz, Sergio

2017-03-01

The aim of this work was to construct InverPep, a database specialised in experimentally validated antimicrobial peptides (AMPs) from invertebrates. AMP data contained in InverPep were manually curated from other databases and the scientific literature. MySQL was integrated with the development platform Laravel; this framework allows to integrate programming in PHP with HTML and was used to design the InverPep web page's interface. InverPep contains 18 separated fields, including InverPep code, phylum and species source, peptide name, sequence, peptide length, secondary structure, molar mass, charge, isoelectric point, hydrophobicity, Boman index, aliphatic index and percentage of hydrophobic amino acids. CALCAMPI, an algorithm to calculate the physicochemical properties of multiple peptides simultaneously, was programmed in PERL language. To date, InverPep contains 702 experimentally validated AMPs from invertebrate species. All of the peptides contain information associated with their source, physicochemical properties, secondary structure, biological activity and links to external literature. Most AMPs in InverPep have a length between 10 and 50 amino acids, a positive charge, a Boman index between 0 and 2 kcal/mol, and 30-50% hydrophobic amino acids. InverPep includes 33 AMPs not reported in other databases. Besides, CALCAMPI and statistical analysis of InverPep data is presented. The InverPep database is available in English and Spanish. InverPep is a useful database to study invertebrate AMPs and its information could be used for the design of new peptides. The user-friendly interface of InverPep and its information can be freely accessed via a web-based browser at http://ciencias.medellin.unal.edu.co/gruposdeinvestigacion/prospeccionydisenobiomoleculas/InverPep/public/home_en. Copyright © 2016 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

EFRT M12 Issue Resolution: Comparison of PEP and Bench-Scale Oxidative Leaching Results

Energy Technology Data Exchange (ETDEWEB)

Rapko, Brian M.; Brown, Christopher F.; Eslinger, Paul W.; Fountain, Matthew S.; Hausmann, Tom S.; Huckaby, James L.; Hanson, Brady D.; Kurath, Dean E.; Minette, Michael J.

2009-08-14

Pacific Northwest National Laboratory (PNNL) has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to perform research and development activities to resolve technical issues identified for the Pretreatment Facility (PTF). The Pretreatment Engineering Platform (PEP) was designed and constructed and is to be operated as part of a plan to respond to issue M12, “Undemonstrated Leaching Processes.” The PEP is a 1/4.5-scale test platform designed to simulate the WTP pretreatment caustic leaching, oxidative leaching, ultrafiltration solids concentration, and slurry washing processes. The PEP replicates the WTP leaching processes using prototypic equipment and control strategies. The PEP also includes non-prototypic ancillary equipment to support the core processing. Two operating scenarios are currently being evaluated for the ultrafiltration process (UFP) and leaching operations. The first scenario has caustic leaching performed in the UFP-2 ultrafiltration feed vessels (i.e., vessel UFP-VSL-T02A in the PEP; and vessels UFP-VSL-00002A and B in the WTP PTF). The second scenario has caustic leaching conducted in the UFP-1 ultrafiltration feed preparation vessels (i.e., vessels UFP-VSL-T01A and B in the PEP; vessels UFP-VSL-00001A and B in the WTP PTF). In both scenarios, 19-M sodium hydroxide solution (NaOH, caustic) is added to the waste slurry in the vessels to dissolve solid aluminum compounds (e.g., gibbsite, boehmite). Caustic addition is followed by a heating step that uses direct steam injection to accelerate the leaching process. Following the caustic leach, the vessel contents are cooled using vessel cooling jackets and/or external heat exchangers. The main difference between the two scenarios is that for leaching in UFP1, the 19-M NaOH is added to un-concentrated waste slurry (3 to 8 wt% solids), while for leaching in UFP2, the slurry is concentrated to nominally

Skew quad compensation at PEP

International Nuclear Information System (INIS)

Murray, J.J.

1977-10-01

Rotational and focal effects of solenoids used in PEP detectors will cause severe perturbations of machine beam optics and must be corrected. Ordinarily this would be accomplished by the addition of compensating solenoids and adjustment of insertion quadrupole strengths. It has been found that an arbitrary cross plane coupling representing the effects of solenoids and/or skew quads in any combination can be synthesized (or compensated) exactly using a quartet of skew quads combined with other erect transport elements in a wide variety of configurations. Specific skew quad compensating systems for PEP have been designed and are under study by PEP staff. So far no fundamental flaws have been discovered. In view of that, PEP management has tentatively authorized the use of such a system in the PEP-4, PEP-9 experiments and proposes to leave the question open ''without prejudice'' for other experiments. Use of skew quad compensation involves an imponderable risk, of course, simply because the method is new and untested. But in addition to providing the only known method for dealing with skew quad perturbations, skew quad compensation, as an alternate to compensating solenoids, promises to be much cheaper, to require much less power and to occupy much less space in the IR's. The purpose of this note is to inform potential users of the foregoing situation and to explain skew quad compensation more fully. 2 refs., 1 fig., 1 tab

Collimation issues for the PEP-II B-factory

International Nuclear Information System (INIS)

Sullivan, M.

1997-12-01

This note describes how beam collimation affects detector backgrounds at the collision point for the PEP-II B-factory, a joint effort of three laboratories: LBNL, LLNL, and SLAC. Beam collimation controls the transverse size as well as the maximum allowed energy spread of the beam. The location of synchrotron radiation masks is determined by the transverse size of the beam in that the masks must prevent radiation generated by beam particles located at large transverse beam positions from directly striking the detector beam pipe. Collimation of the energy spread of the beam is important in the control of backgrounds produced by beam particles that strike a gas molecule (lost beam particles). The author describes some preliminary information from background studies during the first months of commissioning the high energy ring of the PEP-II B-factory and present some model predictions for synchrotron radiation backgrounds when collimators are not present

Responses of mRNA expression of PepT1 in small intestine to ...

African Journals Online (AJOL)

STORAGESEVER

2009-05-04

May 4, 2009 ... 2National Key Laboratory of Animal Nutrition, Beijing, 100193, China. 3College ... porters are present in tissues of sheep, cows, pigs and .... PepT1; Peptide transporter, GAPDH; Glyceraldehyde-3-phosphate dehydrogenase.

The Danish PEP registry: experience with the use of postexposure prophylaxis (PEP) following sexual exposure to HIV from 1998 to 2006

DEFF Research Database (Denmark)

Lunding, Suzanne; Katzenstein, Terese L; Kronborg, Gitte

2010-01-01

BACKGROUND: Studies indicate that antiretroviral postexposure prophylaxis (PEP) after sexual exposure to HIV reduce the risk of infection considerably. Since 1998 PEP after sexual HIV exposure within the preceding 24 hours, has been available in Denmark. PEP can only be prescribed at clinical...... centers with specialists experienced in HIV treatment. The objective of this study is to describe the use of PEP after sexual exposure from 1998 to 2006. METHODS: The Danish PEP registry collects data from all cases of PEP use in Denmark after exposure to HIV through a structured questionnaire. RESULTS......: There were 374 cases of PEP use after sexual exposure. The incidence increased from 5 cases in 1997 to 87 in 2006. PEP was used by heterosexuals (40%) as well as men who have sex with men (57%). The HIV-status of the source was unknown in 41% of the cases of which 90% involved a source belonging to a high...

Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae.

OpenAIRE

Srivastava, A; Woolford, C A; Jones, E W

2000-01-01

Pep3p and Pep5p are known to be necessary for trafficking of hydrolase precursors to the vacuole and for vacuolar biogenesis. These proteins are present in a hetero-oligomeric complex that mediates transport at the vacuolar membrane. PEP5 interacts genetically with VPS8, implicating Pep5p in the earlier Golgi to endosome step and/or in recycling from the endosome to the Golgi. To understand further the cellular roles of Pep3p and Pep5p, we isolated and characterized a set of pep3 conditional ...

Muon shielding for PEP

International Nuclear Information System (INIS)

Jenkins, T.M.; Thomas, R.H.

1974-01-01

The first stage of construction of PEP will consist of electron and positron storage rings. At a later date a 200 GeV proton storage ring may be added. It is judicious therefore, to ensure that the first and second phases of construction are compatible with each other. One of several factors determining the elevation at which the storage rings will be constructed is the necessity to provide adequate radiation shielding. The overhead shielding of PEP is determined by the reproduction of neutrons in the hadron cascade generated by primary protons lost from the storage ring. The minimum overburden planned for PEP is 5.5 meters of earth (1100 gm cm/sup /minus/2/). To obtain a rough estimate of the magnitude of the muon radiation problem this note presents some preliminary calculations. Their purpose is intended merely to show that the presently proposed design for PEP will present no major shielding problems should the protons storage ring be installed. More detailed calculations will be made using muon yield computer codes developed at CERN and NAL and muon transport codes developed at SLAC, when details of the proton storage ring become settled. 9 refs., 4 figs

SwePep, a database designed for endogenous peptides and mass spectrometry.

Science.gov (United States)

Fälth, Maria; Sköld, Karl; Norrman, Mathias; Svensson, Marcus; Fenyö, David; Andren, Per E

2006-06-01

A new database, SwePep, specifically designed for endogenous peptides, has been constructed to significantly speed up the identification process from complex tissue samples utilizing mass spectrometry. In the identification process the experimental peptide masses are compared with the peptide masses stored in the database both with and without possible post-translational modifications. This intermediate identification step is fast and singles out peptides that are potential endogenous peptides and can later be confirmed with tandem mass spectrometry data. Successful applications of this methodology are presented. The SwePep database is a relational database developed using MySql and Java. The database contains 4180 annotated endogenous peptides from different tissues originating from 394 different species as well as 50 novel peptides from brain tissue identified in our laboratory. Information about the peptides, including mass, isoelectric point, sequence, and precursor protein, is also stored in the database. This new approach holds great potential for removing the bottleneck that occurs during the identification process in the field of peptidomics. The SwePep database is available to the public.

The Danish PEP registry: experience with the use of postexposure prophylaxis (PEP) following sexual exposure to HIV from 1998 to 2006

DEFF Research Database (Denmark)

Lunding, Suzanne; Katzenstein, Terese L; Kronborg, Gitte

2010-01-01

centers with specialists experienced in HIV treatment. The objective of this study is to describe the use of PEP after sexual exposure from 1998 to 2006. METHODS: The Danish PEP registry collects data from all cases of PEP use in Denmark after exposure to HIV through a structured questionnaire. RESULTS......: There were 374 cases of PEP use after sexual exposure. The incidence increased from 5 cases in 1997 to 87 in 2006. PEP was used by heterosexuals (40%) as well as men who have sex with men (57%). The HIV-status of the source was unknown in 41% of the cases of which 90% involved a source belonging to a high...... risk group, and 63% involved exposure by receptive anal intercourse. PEP was administered within 24 hours in 95% of the cases and the median time to initiation (N = 225) was 11.0 hours (range 0.5-60.0). PEP was completed by 65%. CONCLUSIONS: This nationwide study showed a steady but moderate increase...

The Woods Hole Partnership Education Program (PEP): Broadening Participation in the Geosciences

Science.gov (United States)

Scott, O.; Jearld, A., Jr.; Liles, G.; Gutierrez, B.

2015-12-01

In March 2009, the Woods Hole Diversity Initiative launched the Partnership Education Program (PEP), a multi-institutional effort to increase diversity in the student population (and ultimately the work force) in the Woods Hole science community. PEP, a summer research internship program, is open to students of all backgrounds but is designed especially to provide opportunities for URM in science, technology, engineering, and mathematics (STEM). PEP is a 10-week program which provides intensive mentored research, a credit-bearing course and supplemental career and professional development activities. Students have opportunities to work in various research areas of geosciences. PEP is emerging as an effective and sustainable approach to bringing students into the STEM research community. PEP is carefully structured to provide critical support for students as they complete their undergraduate experience and prepare for geosciences careers and/or graduate school. The PEP experience is intended to provide students with an entry into the Woods Hole science community, one of the most vibrant marine and environmental research communities in the world. The program aims to provide a first-hand introduction to emerging issues and real-world training in the research skills that students need to advance in science, either as graduate students or bachelors-level working scientists. This is a long-recognized need and efforts are being made to ensure that the students begin to acquire skills and aptitudes that position them to take advantage of a wide range of opportunities. Of note is that the PEP is transitioning into a two year program where students are participating in a second year as a research intern or employee. Since 2013, at least four partner institutions have invited PEP alumni to participate in their respective programs as research assistants and/or full-time technicians.

An asymmetric B factory based on PEP

International Nuclear Information System (INIS)

1991-02-01

In this report we describe a design for a high-luminosity Asymmetric B Factory to be built in the PEP tunnel on the SLAC site. This proposal, a collaborative effort SLAC, LBL, and LLNL, is the culmination of more than two years of effort aimed at the design and construction of an asymmetric e + e - collider capable of achieving a luminosity of L = 3 x 10 33 cm -2 s -1 . The configuration adopted utilizes two storage rings, and electron ring operating at 9 GeV and a positron ring at 3.1 GeV, each with a circumference of 2200 m. The high-energy ring is an upgrade of the PEP storage ring at SLAC; all PEP magnets and most power supplies will be reused. The upgrade consists primarily of replacing the PEP vacuum chamber and RF system with newly designed versions optimized for the high-current environment of the B Factory. The low-energy ring will be newly constructed and will be situated atop the high-energy ring in the PEP tunnel. Utilities already installed in the PEP tunnel are largely sufficient to operate the two B Factory storage rings

PEP-II injection timing and controls

International Nuclear Information System (INIS)

Bharadwaj, V.; Browne, M.; Crane, M.; Gromme, T.; Himel, T.; Ross, M.; Stanek, M.; Ronan, M.

1997-07-01

Hardware has been built and software written and incorporated in the existing SLC accelerator control system to control injection of beam pulses from the accelerator into the PEP-II storage rings currently under construction. Hardware includes a CAMAC module to delay the machine timing fiducial in order that a beam pulse extracted from a damping ring will be injected into a selected group of four 476 MHz buckets in a PEP-II ring. Further timing control is accomplished by shifting the phase of the bunches stored in the damping rings before extraction while leaving the phase of the PEP-II stored beam unchanged. The software which drives timing devices on a pulse-to-pulse basis relies on a dedicated communication link on which one scheduling microprocessor broadcasts a 128-bit message to all distributed control microprocessors at 360 Hz. PEP-II injection will be driven by the scheduling microprocessor according to lists specifying bucket numbers in arbitrary order, and according to scheduling constraints maximizing the useful beam delivered to the SLC collider currently in operation. These lists will be generated by a microprocessor monitoring the current stored per bucket in each of the PEP-II rings

Background sources at PEP

International Nuclear Information System (INIS)

Lynch, H.; Schwitters, R.F.; Toner, W.T.

1988-01-01

Important sources of background for PEP experiments are studied. Background particles originate from high-energy electrons and positrons which have been lost from stable orbits, γ-rays emitted by the primary beams through bremsstrahlung in the residual gas, and synchrotron radiation x-rays. The effect of these processes on the beam lifetime are calculated and estimates of background rates at the interaction region are given. Recommendations for the PEP design, aimed at minimizing background are presented. 7 figs., 4 tabs

An asymmetric B factory based on PEP

Energy Technology Data Exchange (ETDEWEB)

1991-02-01

In this report we describe a design for a high-luminosity Asymmetric B Factory to be built in the PEP tunnel on the SLAC site. This proposal, a collaborative effort SLAC, LBL, and LLNL, is the culmination of more than two years of effort aimed at the design and construction of an asymmetric e{sup +}e{sup {minus}} collider capable of achieving a luminosity of L = 3 {times} 10{sup 33} cm{sup {minus}2} s{sup {minus}1}. The configuration adopted utilizes two storage rings, and electron ring operating at 9 GeV and a positron ring at 3.1 GeV, each with a circumference of 2200 m. The high-energy ring is an upgrade of the PEP storage ring at SLAC; all PEP magnets and most power supplies will be reused. The upgrade consists primarily of replacing the PEP vacuum chamber and RF system with newly designed versions optimized for the high-current environment of the B Factory. The low-energy ring will be newly constructed and will be situated atop the high-energy ring in the PEP tunnel. Utilities already installed in the PEP tunnel are largely sufficient to operate the two B Factory storage rings.

New particle searches at PEP

International Nuclear Information System (INIS)

Berley, D.; Bulos, F.; Cheng, D.

1988-01-01

The new particles that could be produced at PEP are discussed in terms of their specific signatures and production rates. We find that a small number of general signatures characterize these particles. Backgrounds associated with the general signatures are considered and necessary rejection rates are calculated. We describe several typical detectors and tabulate the requirements they place on the PEP machine and the experimental areas. 10 figs., 2 tabs

Luminosity upgrade possibilities for the PEP-II B-Factory

CERN Document Server

Sullivan, M

2003-01-01

PEP-II is an asymmetric e sup + e sup - collider being constructed in the SLAC PEP tunnel by SLAC, LBNL, and LLNL. The two beams have energies of 3.1 GeV and 9.0 GeV and are made to collide at a single interaction point. PEP-II has a 2200 m circumference. The nominal parameters for PEP-II are listed in Table 1. The High Energy Ring (HER) of PEP-II started commissioning in 1997. The Low Energy Ring (LER) will be commissioned in the summer of 1998. The BaBar detector is to be installed starting January 1999. Studies for increasing the luminosity in PEP-II beyond the design are underway. A brief summary of the possibilities are presented here. Improvements to the integrated luminosity will be implemented gradually. Major luminosity improvements will likely come in two phased upgrades. Several of these possibilities are summarized in Table 1.

LUMINOSITY UPGRADE POSSIBILITIES FOR THE PEP-II B-FACTORY

International Nuclear Information System (INIS)

Sullivan, Michael K

2003-01-01

PEP-II is an asymmetric e + e - collider being constructed in the SLAC PEP tunnel by SLAC, LBNL, and LLNL. The two beams have energies of 3.1 GeV and 9.0 GeV and are made to collide at a single interaction point. PEP-II has a 2200 m circumference. The nominal parameters for PEP-II are listed in Table 1. The High Energy Ring (HER) of PEP-II started commissioning in 1997. The Low Energy Ring (LER) will be commissioned in the summer of 1998. The BaBar detector is to be installed starting January 1999. Studies for increasing the luminosity in PEP-II beyond the design are underway. A brief summary of the possibilities are presented here. Improvements to the integrated luminosity will be implemented gradually. Major luminosity improvements will likely come in two phased upgrades. Several of these possibilities are summarized in Table 1

PEP-II: An asymmetric B factory. Conceptual design report

Energy Technology Data Exchange (ETDEWEB)

1993-06-01

In this report, the authors have described an updated conceptual design for the high-luminosity Asymmetric B Factory (PEP-II) to be built in the PEP tunnel culmination of more than four years of effort aimed at the design and construction of an asymmetric e{sub +}e{sub {minus}} collider capable of achieving a luminosity of L = 3 {times} 10{sup 33} cm{sup {minus}2} s{sup {minus}1}. All aspects of the conceptual design were scrutinized in March 1991 by a DOE technical review committee chaired by Dr. L. Edward Temple. The design was deemed feasible and capable of achieving its physics goals. Furthermore, the cost estimate, schedule, and management plan for the project were fully endorsed by the committee. This updated conceptual design report captures the technical progress since the March 1991 review and reflects the lower cost estimate corresponding to the improved design. Although the PEP-II design has continued to evolve, no technical scope changes have been made that invalidate the conclusion of the DOE review. The configuration adopted utilizes two storage rings, an electron ring operating at 9 GeV and a positron ring at 3.1 GeV, each with a circumference of 2200 m. The high-energy ring is an upgrade of the PEP storage ring at SLAC; all PEP magnets and most power supplies will be reused. The upgrade consists primarily of replacing the PEP vacuum chamber and RF system with newly designed versions optimized for the high-current environment of PEP-II. The low-energy ring will be newly constructed and will be situated atop the high-energy ring in the PEP tunnel. Utilities already installed in the PEP tunnel are largely sufficient to operate the two PEP-II storage rings.

Luminosity with more bunches in PEP

International Nuclear Information System (INIS)

Corbett, W.J.

1990-12-01

The near term accelerator physics program for PEP includes experiments in a collider mode with up to 9 bunches in each beam. In this memo, luminosity data from the 3 x 3 configuration is first used to calculate vertical beam size, emittance and tune shift as a function of current. The data is then used to extrapolate to the case with either 6 x 6 or 9 x 9 bunches colliding in PEP. Vertical emittance growth from the separated bunch optics and dispersion at the IP are included in the calculations. The conclusion is that given a 90 mA current drive limitation in PEP, operating with 6 x 6 bunches yields the maximum luminosity. 9 refs., 6 figs

Butyrate transcriptionally enhances peptide transporter PepT1 expression and activity.

Directory of Open Access Journals (Sweden)

Guillaume Dalmasso

Full Text Available BACKGROUND: PepT1, an intestinal epithelial apical di/tripeptide transporter, is normally expressed in the small intestine and induced in colon during chronic inflammation. This study aimed at investigating PepT1 regulation by butyrate, a short-chain fatty acid produced by commensal bacteria and accumulated inside inflamed colonocyte. RESULTS: We found that butyrate treatment of human intestinal epithelial Caco2-BBE cells increased human PepT1 (hPepT1 promoter activity in a dose- and time-dependent manner, with maximal activity observed in cells treated with 5 mM butyrate for 24 h. Under this condition, hPepT1 promoter activity, mRNA and protein expression levels were increased as assessed by luciferase assay, real-time RT-PCR and Western blot, respectively. hPepT1 transport activity was accordingly increased by approximately 2.5-fold. Butyrate did not alter hPepT1 mRNA half-life indicating that butyrate acts at the transcriptional level. Molecular analyses revealed that Cdx2 is the most important transcription factor for butyrate-induced increase of hPepT1 expression and activity in Caco2-BBE cells. Butyrate-activated Cdx2 binding to hPepT1 promoter was confirmed by gel shift and chromatin immunoprecipitation. Moreover, Caco2-BBE cells overexpressing Cdx2 exhibited greater hPepT1 expression level than wild-type cells. Finally, treatment of mice with 5 mM butyrate added to drinking water for 24 h increased colonic PepT1 mRNA and protein expression levels, as well as enhanced PepT1 transport activity in colonic apical membranes vesicles. CONCLUSIONS: Collectively, our results demonstrate that butyrate increases PepT1 expression and activity in colonic epithelial cells, which provides a new understanding of PepT1 regulation during chronic inflammation.

P.E.P

International Nuclear Information System (INIS)

Paterson, J.M.

1980-07-01

The design and construction of the PEP project is briefly reviewed. The initial testing of the storage ring system and its present performance is described. The short-range plans for continuing development are discussed

Recent high precision surveys at PEP

International Nuclear Information System (INIS)

Sah, R.C.

1980-12-01

The task of surveying and aligning the components of PEP has provided an opportunity to develop new instruments and techniques for the purpose of high precision surveys. The new instruments are quick and easy to use, and they automatically encode survey data and read them into the memory of an on-line computer. When measurements of several beam elements have been taken, the on-line computer analyzes the measured data, compares them with desired parameters, and calculates the required adjustments to beam element support stands

The PEP-II/BaBar Project-Wide Database using World Wide Web and Oracle*Case

International Nuclear Information System (INIS)

Chan, A.; Crane, G.; MacGregor, I.; Meyer, S.

1995-12-01

The PEP-II/BaBar Project Database is a tool for monitoring the technical and documentation aspects of the accelerator and detector construction. It holds the PEP-II/BaBar design specifications, fabrication and installation data in one integrated system. Key pieces of the database include the machine parameter list, components fabrication and calibration data, survey and alignment data, property control, CAD drawings, publications and documentation. This central Oracle database on a UNIX server is built using Oracle*Case tools. Users at the collaborating laboratories mainly access the data using World Wide Web (WWW). The Project Database is being extended to link to legacy databases required for the operations phase

Critical Role of PepT1 in Promoting Colitis-Associated Cancer and Therapeutic Benefits of the Anti-inflammatory PepT1-Mediated Tripeptide KPV in a Murine ModelSummary

Directory of Open Access Journals (Sweden)

Emilie Viennois

2016-05-01

Full Text Available Background & Aims: The human intestinal peptide transporter 1 (hPepT1, is expressed in the small intestine at low levels in the healthy colon and up-regulated during inflammatory bowel disease. hPepT1 plays a role in mouse colitis and human studies have shown that chronic intestinal inflammation leads to colorectal cancer (colitis-associated cancer; CAC. Hence, we assessed here the role of PepT1 in CAC. Methods: Mice with hPepT1 overexpression in intestinal epithelial cells (transgenic [TG] or PepT1 (PepT1-knockout [KO] deletion were used and CAC was induced by azoxymethane/dextran sodium sulfate. Results: TG mice had larger tumor sizes, increased tumor burdens, and increased intestinal inflammation compared with wild-type (WT mice. Conversely, tumor number and size and intestinal inflammation were decreased significantly in PepT1-KO mice. Proliferating crypt cells were increased in TG mice and decreased in PepT1-KO mice. Analysis of human colonic biopsy specimens showed increased expression of PepT1 in patients with colorectal cancer, suggesting that PepT1 might be targeted for the treatment of CAC. The use of an anti-inflammatory tripeptide Lys-Pro-Val (KPV transported by PepT1 was able to prevent carcinogenesis in WT mice. When administered to PepT1-KO mice, KPV did not trigger any of the inhibitory effect on tumorigenesis observed in WT mice. Conclusions: The observations that PepT1 was highly expressed in human colorectal tumor and that its overexpression and deletion in mice increased and decreased colitis-associated tumorigenesis, respectively, suggest that PepT1 is a potential therapeutic target for the treatment of colitis-associated tumorigenesis. Keywords: Colitis-Associated Cancer, Intestinal Inflammation, PepT1, KPV Peptide

Estimate of coherent tune shifts for PEP

International Nuclear Information System (INIS)

Yao, C.Y.; Chao, A.W.

1981-02-01

Transverse and longitudinal instabilities for a bunched PEP beam with a Gaussian distribution are treated using the standard technique in which instability problems are solved by looking for eigenvalues of the linearized Vlasov equation. The eigen solutions are conveniently expanded in terms of the Laquerre polynomials, and the eigenvalues are given by a symmetric matrix whose elements can be expressed in infinite series. The well-known formalism is used to obtain the matrix formula, and then applied numerically to the PEP ring to estimate the transverse coherent tune shifts. The impedance used is that estimated for the PEP RF cavities. The agreement with experimental data seems reasonable

Storage rings, internal targets and PEP

International Nuclear Information System (INIS)

Spencer, J.E.

1986-11-01

Storage rings with internal targets are described, using PEP as an example. The difference between electrons and heavier particles such as protons, antiprotons, and heavy ions is also discussed because it raises possibilities of bypass insertions for more exotic experiments. PEP is compared to other rings in various contexts to verify the assertion that it is an ideal ring for many fundamental and practical applications that can be carried on simultaneously

Ca 2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca 2+ channels

KAUST Repository

Qia, Zhi

2010-11-18

A family of peptide signaling molecules (AtPeps) and their plasma membrane receptor AtPepR1 are known to act in pathogendefense signaling cascades in plants. Little is currently known about the molecular mechanisms that link these signaling peptides and their receptor, a leucine-rich repeat receptor-like kinase, to downstream pathogen-defense responses. We identify some cellular activities of these molecules that provide the context for a model for their action in signaling cascades. AtPeps activate plasma membrane inwardly conducting Ca 2+ permeable channels in mesophyll cells, resulting in cytosolic Ca 2+ elevation. This activity is dependent on their receptor as well as a cyclic nucleotide-gated channel (CNGC2). We also show that the leucine-rich repeat receptor- like kinase receptor AtPepR1 has guanylyl cyclase activity, generating cGMP from GTP, and that cGMP can activate CNGC2- dependent cytosolic Ca 2+ elevation. AtPep-dependent expression of pathogen-defense genes (PDF1.2, MPK3, and WRKY33) is mediated by the Ca 2+ signaling pathway associated with AtPep peptides and their receptor. The work presented here indicates that extracellular AtPeps, which can act as danger-associated molecular patterns, signal by interaction with their receptor, AtPepR1, a plasma membrane protein that can generate cGMP. Downstream from AtPep and AtPepR1 in a signaling cascade, the cGMP-activated channel CNGC2 is involved in AtPep- and AtPepR1-dependent inward Ca 2+ conductance and resulting cytosolic Ca 2+ elevation. The signaling cascade initiated by AtPeps leads to expression of pathogen- defense genes in a Ca 2+-dependent manner.

Single wavelength standard wiggler for PEP

International Nuclear Information System (INIS)

Brunk, W.; Fischer, G.; Spencer, J.

1979-03-01

A 1lambda planar wiggler has been designed that will be used for the initial operation of the 4 to 18 GeV storage ring PEP. Three of these wigglers will be installed symmetrically around the ring at 120 0 intervals in three of six available 5 m straight sections with the purpose of providing: (1) beam size control to obtain better luminosities below 15 GeV, and (2) decreased damping times to obtain better injection rates at lower energies. Design goals are discussed and a description of the final system including cost estimates is given. Expected results and usage in PEP are discussed. Some possibilities for production of synchrotron radiation and beam monitoring with shorter wavelength, multiple-period wigglers at PEP energies are also discussed. Comparison to a wiggler now operating in SPEAR is given

Status of PEP and TPC/2γ

International Nuclear Information System (INIS)

Zapalac, G.

1989-06-01

The TPC/2γ program at PEP has been upgraded by increasing the PEP luminosity and by the adding of a vertex chamber to the TPC detector. These improvements will allow a strong program in B and τ physics, and will contribute to ongoing studies in two-photon physics and hadronization. 10 refs., 3 figs

PEP Integrated Test D Run Report Caustic and Oxidative Leaching in UFP-VSL-T02A

Energy Technology Data Exchange (ETDEWEB)

Sevigny, Gary J.; Bredt, Ofelia P.; Burns, Carolyn A.; Kurath, Dean E.; Geeting, John GH; Golovich, Elizabeth C.; Guzman-Leong, Consuelo E.; Josephson, Gary B.

2009-12-11

Pacific Northwest National Laboratory (PNNL) has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to perform research and development activities to resolve technical issues identified for the Pretreatment Facility (PTF). The Pretreatment Engineering Platform (PEP) was designed, constructed and operated as part of a plan to respond to issue M12, "Undemonstrated Leaching Processes" of the External Flowsheet Review Team (EFRT) issue response plan. The PEP is a 1/4.5-scale test platform designed to simulate the WTP pretreatment caustic leaching, oxidative leaching, ultrafiltration solids concentration, and slurry washing processes. The PEP replicates the WTP leaching processes using prototypic equipment and control strategies. The PEP also includes non-prototypic ancillary equipment to support the core processing. Two operating scenarios are currently being evaluated for the ultrafiltration process (UFP) and leaching operations. The first scenario (Test B and D) has caustic leaching performed in the UFP-2 ultrafiltration feed vessels (i.e., vessel UFP-VSL-T02A in the PEP and vessels UFP-VSL-00002A and B in the WTP PTF). The second scenario (Test A) has caustic leaching conducted in the UFP-1 ultrafiltration feed preparation vessels (i.e., vessels UFP-VSL-T01A and B in the PEP and vessels UFP VSL-00001A and B in the WTP PTF). In Test D, 19M sodium hydroxide (NaOH, caustic) was added to the waste slurry in the UFP VSL T02 vessel after the solids were concentrated to ~20% undissolved solids. The NaOH was added to leach solid aluminum compounds (e.g., gibbsite, boehmite). Caustic addition is followed by heating to 85°C using direct injection of steam to accelerate the leach process. The main difference of Test D compared to Test B is that the leach temperature is 85°C for 24 hrs as compared to 100°C for 12 hours. The other difference is the Test D simulant had Cr in the

PEP-II prototype klystron

International Nuclear Information System (INIS)

Fowkes, W.R.; Caryotakis, G.; Lee, T.G.; Pearson, C.; Wright, E.L.

1993-04-01

A 540-kW continuous-wave (cw) klystron operating at 476 MHz was developed for use as a power source for testing PEP-II rf accelerating cavities and rf windows. It also serves as a prototype for a 1.2 MW cw klystron presently being developed as a potential rf source for asymmetric colliding ring use. The design incorporates the concepts and many of the parts used in the original 353 MHz PEP klystron developed sixteen years ago. The superior computer simulation codes available today result in improved performance with the cavity frequencies, drift lengths, and output circuit optimized for the higher frequency.The design and operating results of this tube are described with particular emphasis on the factors which affect efficiency and stability

Geotechnical investigations of the PEP site

International Nuclear Information System (INIS)

Gould, R.S.

1976-02-01

The purpose of this paper is to summarize the general nature of the geology and rock and soil formations of the PEP site as they relate to the design and construction of the project; to describe site investigation programs and to catalog the geotechnical information presently available about the site. The recently-completed investigation of subterranean conditions around the PEP ring when coupled with previous surveys gives us a good understanding of what to expect with regard to tunneling, undertaking larger underground excavations and constructing research halls are the interaction areas. It bears out the predictions made in Jacobs and Associates' report of 1973; i.e., that the ring housing construction is classified as soft-ground tunneling and that large underground openings, such as region 10 and the injection junction structures, will require great attention to support. A shield or shields will probably be required. On the positive side, the site affords very good conditions for soft-ground tunneling. Water will be a problem in some areas, but not an unsolvable one. The possibility of encountering lethal or explosive gases, almost always the case in tunneling in California's coastal formations, exists but has not been ascertained. Finally, no reasons to change current cost estimates or schedules have merged from the investigation. 13 refs., 1 fig

Development of HyPEP, A Hydrogen Production Plant Efficiency Calculation Program

International Nuclear Information System (INIS)

Lee, Young Jin; Park, Ji Won; Lee, Won Jae; Shin, Young Joon; Kim, Jong Ho; Hong, Sung Deok; Lee, Seung Wook; Hwang, Moon Kyu

2007-12-01

Development of HyPEP program for assessing the steady-state hydrogen production efficiency of the nuclear hydrogen production facilities was carried out. The main developmental aims of the HyPEP program are the extensive application of the GUI for enhanced user friendliness and the fast numerical solution scheme. These features are suitable for such calculations as the optimisation calculations. HyPEP was developed with the object-oriented programming techniques. The components of the facility was modelled as objects in a hierarchical structure where the inheritance property of the object oriented program were extensively applied. The Delphi program language which is based on the Object Pascal was used for the HyPEP development. The conservation equations for the thermal hydraulic flow network were setup and the numerical solution scheme was developed and implemented into HyPEP beta version. HyPEP beta version has been developed with working GUI and the numerical solution scheme implementation. Due to the premature end of this project the fully working version of HyPEP was not produced

Configuring the SLC linac for injection into PEP

International Nuclear Information System (INIS)

Bane, K.L.F.

1989-01-01

From time to time the normal SLC physics program is to be interrupted so that beam can be delivered to PEP. In order that the switch to PEP injection (and the switch back again) can be accomplished quickly and easily, the gun, the damping rings, the linac phase ramp, the energy profile of the linac klystrons for the scavenger bunch, and the entire positron production system are to be kept the same as in the SLC configuration. What mainly remains to be changed is the linac klystron profile for the leading two bunches - those going to PEP. The new klystron profile must be such that it leaves these two beams (1) with final energies that match that of the storage ring and (2) with final energy spectra that fit within the energy aperture of the PEP transfer line. The conditions that need to be met in order to achieve these two goals are discussed in this note. 1 ref., 2 figs

X-prolyl dipeptidyl aminopeptidase gene (pepX) is part of the glnRA operon in Lactobacillus rhamnosus.

Science.gov (United States)

Varmanen, P; Savijoki, K; Avall, S; Palva, A; Tynkkynen, S

2000-01-01

A peptidase gene expressing X-prolyl dipeptidyl aminopeptidase (PepX) activity was cloned from Lactobacillus rhamnosus 1/6 by using the chromogenic substrate L-glycyl-L-prolyl-beta-naphthylamide for screening of a genomic library in Escherichia coli. The nucleotide sequence of a 3.5-kb HindIII fragment expressing the peptidase activity revealed one complete open reading frame (ORF) of 2,391 nucleotides. The 797-amino-acid protein encoded by this ORF was shown to be 40, 39, and 36% identical with PepXs from Lactobacillus helveticus, Lactobacillus delbrueckii, and Lactococcus lactis, respectively. By Northern analysis with a pepX-specific probe, transcripts of 4.5 and 7.0 kb were detected, indicating that pepX is part of a polycistronic operon in L. rhamnosus. Cloning and sequencing of the upstream region of pepX revealed the presence of two ORFs of 360 and 1,338 bp that were shown to be able to encode proteins with high homology to GlnR and GlnA proteins, respectively. By multiple primer extension analyses, the only functional promoter in the pepX region was located 25 nucleotides upstream of glnR. Northern analysis with glnA- and pepX-specific probes indicated that transcription from glnR promoter results in a 2.0-kb dicistronic glnR-glnA transcript and also in a longer read-through polycistronic transcript of 7.0 kb that was detected with both probes in samples from cells in exponential growth phase. The glnA gene was disrupted by a single-crossover recombinant event using a nonreplicative plasmid carrying an internal part of glnA. In the disruption mutant, glnRA-specific transcription was derepressed 10-fold compared to the wild type, but the 7.0-kb transcript was no longer detectable with either the glnA- or pepX-specific probe, demonstrating that pepX is indeed part of glnRA operon in L. rhamnosus. Reverse transcription-PCR analysis further supported this operon structure. An extended stem-loop structure was identified immediately upstream of pepX in the glnA-pep

Seepage into PEP tunnel

International Nuclear Information System (INIS)

Weidner, H.

1990-01-01

The current rate of seepage into the PEP tunnel in the vicinity of IR-10 is very low compared to previous years. Adequate means of handling this low flow are in place. It is not clear whether the reduction in the flow is temporary, perhaps due to three consecutive dry years, or permanent due to drainage of a perched water table. During PEP construction a large amount of effort was expended in attempts to seal the tunnel, with no immediate effect. The efforts to ''manage'' the water flow are deemed to be successful. By covering equipment to protect it from dripping water and channeling seepage into the drainage gutters, the seepage has been reduced to a tolerable nuisance. There is no sure, safe procedure for sealing a leaky shotcreted tunnel

The PEP [positron-electron-proton] electron-positron ring: PEP Stage I

International Nuclear Information System (INIS)

Rees, J.R.

1974-01-01

The first stage of the positron-electron-proton (PEP) colliding-beam system which has been under joint study by a Lawrence Berkeley Laboratory-Stanford Linear Accelerator Center team for the past two years, will be the electron-positron storage ring. The physics justification for the e/sup /plus//e/sup /minus// ring is summarized briefly and the proposed facility is described. The ring will have six arcs having gross radii of about 220 m and six interaction regions located at the centers of straight sections about 130 m long. The longitudinal distance left free for experimental apparatus around the interaction regions will be 20 m. The range of operating beam energies will be from 5 GeV to 15 GeV. The design luminosity at 15 GeV will be 10 32 cm/sup /minus/2/s/sup/minus/1/, and the luminosity will vary approximately as the square of the beam energy. Alternative methods under consideration for adjusting the beam cross-section are discussed. The designs of the storage ring subsystems and of the conventional facilities including the experimental halls at the interaction regions are described. 7 refs., 8 figs., 3 tabs

Progress of the PEP-II B-factory

International Nuclear Information System (INIS)

Seeman, J.; Browne, M.; Cai, Y.; Colocho, W.; Decker, F.-J.; Donald, M.; Ecklund, S.; Erickson, R.; Fisher, A.; Fox, J.; Heifets, S.; Iverson, R.; Kozanecki, W.; Krejcik, P.; Kulikov, A.; Novokhatski, A.; Schuh, P.; Schwarz, H.; Stanek, M.; Sullivan, M.; Teytelman, D.; Turner, J.; Wienands, U.; Yan, Y.; Yocky, J.; Biagini, M.; Zisman, M.

2003-01-01

PEP-II is an e + e - B-Factory Collider located at SLAC operating at the Upsilon 4S resonance. PEP-II has delivered, over the past five years, an integrated luminosity to the BaBar detector of over 139 fb -1 and has reached a luminosity of 6.58 x 10 36 /cm 2 /s. Steady progress is being made in reaching higher luminosity. The goal over the next several years is to reach a luminosity of at least 2 x 10 34 /cm 2 /s. The accelerator physics issues being addressed in PEP-II to reach this goal include the electron cloud instability, beam-beam effects, parasitic beam-beam effects, high RF beam loading, shorter bunches, lower beta y*, interaction region operation, and coupling control. A view of the PEP-II tunnel is shown in Figure 1. The present parameters of the PEP-II B-Factory are shown in Table 1 compared to the design. The present peak luminosity is 219% of design and the best integrated luminosity per month is 7.4 fb -1 that is 225% of design. The best luminosity per month is shown in Figure 2. The integrated luminosity over a month is shown in Figure 3 and the total integrated luminosity in shown in Figure 4. The progress in luminosity has come from correcting the orbits, adding specific orbit bumps to correct coupling and dispersion issues, lowering the beta y* in the LER, and moving the fractional horizontal tunes in both rings to just above the half integer (<0.52)

PEP-II RF System Operation and Performance

International Nuclear Information System (INIS)

McIntosh, P.

2005-01-01

The Low Energy Ring (LER) and High Energy Ring (HER) RF systems have operated now on PEP-II since July 1998 and have assisted in breaking all design luminosity records back in June 2001. Luminosity on PEP-II has steadily increased since then as a consequence of larger e+ and e- beam currents being accumulated. This has meant that the RF systems have inevitably been driven harder, not only to achieve these higher stored beam currents, but also to reliably keep the beams circulating whilst at the same time minimizing the number of aborts due to RF system faults. This paper details the current PEP-II RF system configurations for both rings, as well as future upgrade plans spanning the next 3-5 years. Limitations of the current RF system configurations are presented, highlighting improvement projects which will target specific areas within the RF systems to ensure that adequate operating overheads are maintained and reliable operation is assured. The Low Energy Ring (LER) and High Energy Ring (HER) RF systems have operated now on PEP-II since July 1998 and have assisted in breaking all design luminosity records back in June 2001. Luminosity on PEP-II has steadily increased since then as a consequence of larger e+ and e- beam currents being accumulated. This has meant that the RF systems have inevitably been driven harder, not only to achieve these higher stored beam currents, but also to reliably keep the beams circulating whilst at the same time minimizing the number of aborts due to RF system faults. This paper details the current PEP-II RF system configurations for both rings, as well as future upgrade plans spanning the next 3-5 years. Limitations of the current RF system configurations are presented, highlighting improvement projects which will target specific areas within the RF systems to ensure that adequate operating overheads are maintained and reliable operation is assured

PEP-II asymmetric B Factory: Design update and R ampersand D results

International Nuclear Information System (INIS)

Zisman, M.S.; Bell, R.A.; Dorfan, J.; Schwarz, H.; Barletta, W.A.; Calderon, M.

1992-07-01

PEP-II, a 9 GeV x 3.1 GeV e + e - collider with a design luminosity of 3 x 10 33 cm -2 s -1 , was proposed jointly by SLAC, LBL, and LLNL. Recent efforts have continued towards an optimized design. In addition, an aggressive R ampersand D program is under way to validate our design choices. Fabrication of a low-power prototype RF cavity is complete, and impedance measurements are beginning. A 500-kW, 476-MHz klystron has been completed; it will be used for testing both high-power RF windows and a prototype high-power cavity (now under design in collaboration with Chalk River Laboratory). Vacuum studies have demonstrated that chambers with suitable photodesorption properties can be fabricated. A mockup of the two-ring arc area has been completed and used to investigate alignment and stability issues. The PEP-II project is ready to begin construction as soon as funds become available

StraPep: a structure database of bioactive peptides

Science.gov (United States)

Wang, Jian; Yin, Tailang; Xiao, Xuwen; He, Dan; Xue, Zhidong; Jiang, Xinnong; Wang, Yan

2018-01-01

Abstract Bioactive peptides, with a variety of biological activities and wide distribution in nature, have attracted great research interest in biological and medical fields, especially in pharmaceutical industry. The structural information of bioactive peptide is important for the development of peptide-based drugs. Many databases have been developed cataloguing bioactive peptides. However, to our knowledge, database dedicated to collect all the bioactive peptides with known structure is not available yet. Thus, we developed StraPep, a structure database of bioactive peptides. StraPep holds 3791 bioactive peptide structures, which belong to 1312 unique bioactive peptide sequences. About 905 out of 1312 (68%) bioactive peptides in StraPep contain disulfide bonds, which is significantly higher than that (21%) of PDB. Interestingly, 150 out of 616 (24%) bioactive peptides with three or more disulfide bonds form a structural motif known as cystine knot, which confers considerable structural stability on proteins and is an attractive scaffold for drug design. Detailed information of each peptide, including the experimental structure, the location of disulfide bonds, secondary structure, classification, post-translational modification and so on, has been provided. A wide range of user-friendly tools, such as browsing, sequence and structure-based searching and so on, has been incorporated into StraPep. We hope that this database will be helpful for the research community. Database URL: http://isyslab.info/StraPep PMID:29688386

PEP Laser Surveying System

International Nuclear Information System (INIS)

Lauritzen, T.; Sah, R.C.

1979-03-01

A Laser Surveying System has been developed to survey the beam elements of the PEP storage ring. This system provides automatic data acquisition and analysis in order to increase survey speed and to minimize operator error. Two special instruments, the Automatic Readout Micrometer and the Small Automatic Micrometer, have been built for measuring the locations of fiducial points on beam elements with respect to the light beam from a laser. These instruments automatically encode offset distances and read them into the memory of an on-line computer. Distances along the beam line are automatically encoded with a third instrument, the Automatic Readout Tape Unit. When measurements of several beam elements have been taken, the on-line computer analyzes the measured data, compared them with desired parameters, and calculates the required adjustments to beam element support stands

PepJ is a new extracellular proteinase of Aspergillus nidulans.

Science.gov (United States)

Emri, T; Szilágyi, M; László, K; M-Hamvas, M; Pócsi, I

2009-01-01

Under carbon starvation, Aspergillus nidulans released a metallo-proteinase with activities comparable to those of PrtA, the major extracellular serine proteinase of the fungus. The relative molar mass of the enzyme was 19 kDa as determined with both denaturing and renaturing SDS PAGE, while its isoelectric point and pH and temperature optima were 8.6, 5.5 and 65 degrees C, respectively. The enzyme was stable at pH 3.5-10.5 and was still active at 95 degrees C in the presence of azocasein substrate. MALDI-TOF MS analysis demonstrated that the proteinase was encoded by the pepJ gene (locus ID AN7962.3), and showed high similarity to deuterolysin from Aspergillus oryzae. The size of the mature enzyme, its EDTA sensitivity and heat stability also supported the view that A. nidulans PepJ is a deuterolysin-type metallo-proteinase.

Post-Exposure Prophylaxis (PEP)

Science.gov (United States)

... Child Transmission of HIV Post-Exposure Prophylaxis (PEP) Pre-Exposure Prophylaxis (PrEP) HIV Treatment HIV Treatment: The Basics Just ... to HIV frequently. Another HIV prevention method, called pre-exposure prophylaxis or PrEP, is when people at high risk ...

Peptide drug stability: The anti-inflammatory drugs Pep19-2.5 and Pep19-4LF in cream formulation.

Science.gov (United States)

Kuhlmann, Nicole; Heinbockel, Lena; Correa, Wilmar; Gutsmann, Thomas; Goldmann, Torsten; Englisch, Uwe; Brandenburg, Klaus

2018-03-30

In previous years, we developed anti-infective drugs based on antimicrobial peptides (AMPs), which have been shown to effectively block severe infections and inflammation in vitro as well as in vivo. Besides systemic application, the occurrence of severe local infections necessitates a topical application for example in the case of severe skin and soft tissue infections (SSTI). Recent investigations show that the synthetic anti-lipopolysaccharide peptide (SALP) Pep19-2.5 (Aspidasept® I) and a variant called Pep19-4LF (Aspidasept® II) are able to supress inflammation reactions also in keratinocytes, Langerhans cells, and dendritic cells from the skin. For topical application, a possible formulation represents the drug dispersed into a pharmaceutical cream (DAC base cream). Here, we present investigations on the stability of the peptides using this formulation in dependence on time, which includes the evaluation of the extraction procedure, the quantitative analysis of the peptides after extraction, its sensitivity to protease degradation and its ability to maintain activity against LPS-induced inflammation in vitro. We have developed an extraction procedure for the peptides with an optimum yield and showed that Pep19-2.5 is present as a dimer after extraction from the cream, whereas Pep19-4LF retains its monomeric form. Both peptides show no degradation by chymotrypsin after extraction for at least 1 h, which is indicative for an attachment of constituents of the base cream, inhibiting the cutting into peptidic part structures. The extracted peptides and in particular the dimeric Pep19-2.5 are still able to inhibit the LPS-induced inflammation reaction in human mononuclear cells. Copyright © 2018 Elsevier B.V. All rights reserved.

PepArML: A Meta-Search Peptide Identification Platform for Tandem Mass Spectra.

Science.gov (United States)

Edwards, Nathan J

2013-12-01

The PepArML meta-search peptide identification platform for tandem mass spectra provides a unified search interface to seven search engines; a robust cluster, grid, and cloud computing scheduler for large-scale searches; and an unsupervised, model-free, machine-learning-based result combiner, which selects the best peptide identification for each spectrum, estimates false-discovery rates, and outputs pepXML format identifications. The meta-search platform supports Mascot; Tandem with native, k-score and s-score scoring; OMSSA; MyriMatch; and InsPecT with MS-GF spectral probability scores—reformatting spectral data and constructing search configurations for each search engine on the fly. The combiner selects the best peptide identification for each spectrum based on search engine results and features that model enzymatic digestion, retention time, precursor isotope clusters, mass accuracy, and proteotypic peptide properties, requiring no prior knowledge of feature utility or weighting. The PepArML meta-search peptide identification platform often identifies two to three times more spectra than individual search engines at 10% FDR.

Effects of pergolide mesylate on transduction efficiency of PEP-1-catalase protein

International Nuclear Information System (INIS)

Sohn, Eun Jeong; Kim, Dae Won; Kim, Young Nam; Kim, So Mi; Lim, Soon Sung; Kang, Tae-Cheon; Kwon, Hyeok Yil; Kim, Duk-Soo; Cho, Sung-Woo; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik; Hwang, Hyun Sook; Choi, Soo Young

2011-01-01

Research highlights: → We studied effects of pergolide mesylate (PM) on in vitro and in vivo transduction of PEP-1-catalase. → PEP-1-catatase inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation. → PM enhanced the transduction of PEP-1-catalase into HaCaT cells and skin tissue. → PM increased anti-inflammatory activity of PEP-1-catalase. → PM stimulated therapeutic action of anti-oxidant enzyme catalase in oxidative-related diseases. -- Abstract: The low transduction efficiency of various proteins is an obstacle to their therapeutic application. However, protein transduction domains (PTDs) are well-known for a highly effective tool for exogenous protein delivery to cells. We examined the effects of pergolide mesylate (PM) on the transduction of PEP-1-catalase into HaCaT human keratinocytes and mice skin and on the anti-inflammatory activity of PEP-1-catatase against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation using Western blot and histological analysis. PM enhanced the time- and dose-dependent transduction of PEP-1-catalase into HaCaT cells without affecting the cellular toxicity. In a mouse edema model, PEP-1-catalase inhibited the increased expressions of inflammatory mediators and cytokines such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1β, and tumor necrosis factor-α induced by TPA. On the other hand, PM alone failed to exert any significant anti-inflammatory effects. However, the anti-inflammatory effect of co-treatment with PEP-1-catalase and PM was more potent than that of PEP-1-catalase alone. Our results indicate that PM may enhance the delivery of PTDs fusion therapeutic proteins to target cells and tissues and has potential to increase their therapeutic effects of such drugs against various diseases.

Effects of pergolide mesylate on transduction efficiency of PEP-1-catalase protein

Energy Technology Data Exchange (ETDEWEB)

Sohn, Eun Jeong; Kim, Dae Won; Kim, Young Nam; Kim, So Mi [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Lim, Soon Sung [Department of Food Science and Nutrition and RIC Center, Hallym University, Chunchon 200-702 (Korea, Republic of); Kang, Tae-Cheon [Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chunchon 200-702 (Korea, Republic of); Kwon, Hyeok Yil [Department of Physiology, College of Medicine, Hallym University, Chunchon 200-702 (Korea, Republic of); Kim, Duk-Soo [Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 330-090 (Korea, Republic of); Cho, Sung-Woo [Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736 (Korea, Republic of); Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Hwang, Hyun Sook, E-mail: wazzup@hallym.ac.kr [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Choi, Soo Young, E-mail: sychoi@hallym.ac.kr [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of)

2011-03-18

Research highlights: {yields} We studied effects of pergolide mesylate (PM) on in vitro and in vivo transduction of PEP-1-catalase. {yields} PEP-1-catatase inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation. {yields} PM enhanced the transduction of PEP-1-catalase into HaCaT cells and skin tissue. {yields} PM increased anti-inflammatory activity of PEP-1-catalase. {yields} PM stimulated therapeutic action of anti-oxidant enzyme catalase in oxidative-related diseases. -- Abstract: The low transduction efficiency of various proteins is an obstacle to their therapeutic application. However, protein transduction domains (PTDs) are well-known for a highly effective tool for exogenous protein delivery to cells. We examined the effects of pergolide mesylate (PM) on the transduction of PEP-1-catalase into HaCaT human keratinocytes and mice skin and on the anti-inflammatory activity of PEP-1-catatase against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation using Western blot and histological analysis. PM enhanced the time- and dose-dependent transduction of PEP-1-catalase into HaCaT cells without affecting the cellular toxicity. In a mouse edema model, PEP-1-catalase inhibited the increased expressions of inflammatory mediators and cytokines such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1{beta}, and tumor necrosis factor-{alpha} induced by TPA. On the other hand, PM alone failed to exert any significant anti-inflammatory effects. However, the anti-inflammatory effect of co-treatment with PEP-1-catalase and PM was more potent than that of PEP-1-catalase alone. Our results indicate that PM may enhance the delivery of PTDs fusion therapeutic proteins to target cells and tissues and has potential to increase their therapeutic effects of such drugs against various diseases.

Performance of the PEP-II B-Factory Collider at SLAC

International Nuclear Information System (INIS)

Seeman, J.; Browne, M.; Cai, Y.; Colocho, W.; Decker, F.J.; Donald, M.H.; Ecklund, S.; Erickson, R.A.; Fisher, A.S.; Fox, J.D.; Heifets, S.A.; Iverson, R.H.; Kulikov, A.; Li, N.; Novokhatski, A.; Ross, M.C.; Schuh, P.; Smith, T.J.; Sonnad, K.G.; Stanek, M.; Sullivan, M.K.

2006-01-01

PEP-II is an e + e - asymmetric B-Factory Collider located at SLAC operating at the Upsilon 4S resonance (3.1 GeV x 9 GeV). It has reached a luminosity of 9.21 x 10 33 /cm 2 /s and has delivered an integrated luminosity of 710 pb -1 in one day. PEP-II has delivered, over the past six years, an integrated luminosity to the BaBar detector of over 262 fb -1 . PEP-II operates in continuous injection mode for both beams boosting the integrated luminosity. The peak positron current has reached 2.45 A in 1588 bunches. Steady progress is being made in reaching higher luminosity. The goal over the next several years is to reach a luminosity of 2.1 x 10 34 /cm 2 /s. The accelerator physics issues being addressed in PEP-II to reach this goal include the electron cloud instability, beam-beam effects, parasitic beam-beam effects, high RF beam loading, shorter bunches, lower β* y interaction region operation, and coupling control. Figure 1 shows the PEP-II tunnel

Vacuum system for the Stanford-LBL storage ring (PEP)

International Nuclear Information System (INIS)

Bostic, D.; Cummings, U.; Dean, N.; Jeong, B.; Jurow, J.

1975-03-01

The vacuum system for PEP will be similar in design, construction and operation to the system currently in operation at SPEAR. There will, of course, be quantitative differences since the closed path of PEP will be 10 times longer than the SPEAR path. Some qualitative differences will also arise since the radiated synchrotron power for PEP will be about 13 times greater than for SPEAR giving rise to an increased linear power density incident on the chamber wall. Other differences arise from the higher energy spectrum of the synchrotron radiation. The SPEAR vacuum system has been in operation since April 1972 and has proven satisfactory in design, construction and operation. The chamber has been subject to synchrotron radiation for approximately 300 ampere-hours and beam lifetimes are now more than several hours. The details of the PEP design and the SPEAR operating experience will be further discussed in this paper. 1 ref., 4 figs

Operation of PEP longitudinal feedback system

International Nuclear Information System (INIS)

Allen, M.A.; Karvonen, L.G.; McConnell, R.A.; Schwarz, H.

1981-03-01

In order to suppress longitudinal coupled-bunch oscillations which might limit the capabilities of PEP, the 18 GeV e + e - storage ring at SLAC, a longitudinal feedback system is utilized. A frequency domain feedback system was chosen with the frequency spectrum of the stored beam being sampled close to a symmetry point in the ring where the feedback cavity itself is also located. The symmetry point chosen is symmetry point 5 which lies half-way between interaction regions 4 and 6. The system has been installed in PEP and is now operational. However, at stored currents up to the maximum stored in PEP to date at 14.5 GeV (approximately 40 mA in 6 bunches), the ring has been stable to all modes of longitudinal coupled-bunch oscillations both barycentric and the other fundamental modes. By deliberately detuning the main accelerating cavities, small multibunch oscillations can be introduced which, in turn, can be damped by the feedback system. Under optimized beam conditions the feedback system could be adjusted to positive feedback and excite oscillations with relatively small power to the feedback cavity. This will be described along with other details of the system

Knowledge, Attitude and Practice of Post-Exposure Prophylaxis (PEP)

African Journals Online (AJOL)

user

Their levels of knowledge and indications for. PEP as well as ... uninfected patient and even re-infection of an already infected .... Surgical cuts/wounds. 7 ... Table 4: Knowledge of time of administration of PEP. Time .... respondents to be uncertain about the existence of such ... Surely, before the advent of this long awaited.

A missing-bending-magnet scheme for PEP

International Nuclear Information System (INIS)

Liu, R.Z.; Winick, H.

1988-01-01

This article presents a missing-bending-magnet scheme for PEP as a modification that could be considered if PEP were available as a fully dedicated synchrotron radiation source. The scheme can be applied to one or more PEP sextants without changing the rest. By removing some bending magnets, rearranging the remaining magnets, and adding two quadrupoles, ten additional straight sections per sextant can be created, each 5 m or more in length, for insertion devices. Beam lines therefrom, plus possible beam lines from bending magnets would enter a continuous experimental hall instead of individual tunnels and halls for each beam line. This should result in construction cost savings and increased operations efficiency. The ideal beam orbit is unchanged at the two ends and the middle of the sextant. At the end of the curved part of the sextant the lattice functions match those of the long interaction region straight section in the low emittance configuration of PEP. The electron beam characteristics in the newly created straight sections are described, including the enlargement of the horizontal beam size due to the nonzero dispersion. Some disadvantages of the scheme are increased operations complexity due to the need for nine new quadrupole families, increased beam emittance (by 14.5% is one sextant is modified), and reduced dynamic aperture. However, the dynamic aperture is still about as large as the physical aperture and should be adequate for good beam lifetime and injection. (orig.)

Integrated Support Environment (ISE) Laboratory

Data.gov (United States)

Federal Laboratory Consortium — Purpose:The Integrated Support Environment (ISE) Laboratory serves the fleet, in-service engineers, logisticians and program management offices by automatically and...

Positron--Electron Project (PEP)

International Nuclear Information System (INIS)

Rees, J.R.

1977-01-01

PEP, an 18-GeV electron-positron colliding-beam storage ring facility at SLAC, is being built by a team from LBL and SLAC. Construction is under way and completion is scheduled for Fall of 1979. A summary is given of the design of the facility, and the status of the project is reported

Precision surveying system for PEP

International Nuclear Information System (INIS)

Gunn, J.; Lauritzen, T.; Sah, R.; Pellisier, P.F.

1977-01-01

A semi-automatic precision surveying system is being developed for PEP. Reference elevations for vertical alignment will be provided by a liquid level. The short range surveying will be accomplished using a Laser Surveying System featuring automatic data acquisition and analysis

Therapeutical Administration of Peptide Pep19-2.5 and Ibuprofen Reduces Inflammation and Prevents Lethal Sepsis

Science.gov (United States)

Barcena Varela, Sergio; Ferrer-Espada, Raquel; Reiling, Norbert; Goldmann, Torsten; Gutsmann, Thomas; Mier, Walter; Schürholz, Tobias; Drömann, Daniel; Brandenburg, Klaus; Martinez de Tejada, Guillermo

2015-01-01

Sepsis is still a major cause of death and many efforts have been made to improve the physical condition of sepsis patients and to reduce the high mortality rate associated with this disease. While achievements were implemented in the intensive care treatment, all attempts within the field of novel therapeutics have failed. As a consequence new medications and improved patient stratification as well as a thoughtful management of the support therapies are urgently needed. In this study, we investigated the simultaneous administration of ibuprofen as a commonly used nonsteroidal anti-inflammatory drug (NSAID) and Pep19-2.5 (Aspidasept), a newly developed antimicrobial peptide. Here, we show a synergistic therapeutic effect of combined Pep19-2.5-ibuprofen treatment in an endotoxemia mouse model of sepsis. In vivo protection correlates with a reduction in plasma levels of both tumor necrosis factor α and prostaglandin E, as a likely consequence of Pep19-2.5 and ibuprofen-dependent blockade of TLR4 and COX pro-inflammatory cascades, respectively. This finding is further characterised and confirmed in a transcriptome analysis of LPS-stimulated human monocytes. The transcriptome analyses showed that Pep19-2.5 and ibuprofen exerted a synergistic global effect both on the number of regulated genes as well as on associated gene ontology and pathway expression. Overall, ibuprofen potentiated the anti-inflammatory activity of Pep19-2.5 both in vivo and in vitro, suggesting that NSAIDs could be useful to supplement future anti-sepsis therapies. PMID:26197109

Transverse instability excited by rf deflecting modes for PEP

International Nuclear Information System (INIS)

Chao, A.W.; Yao, C.Y.

1979-11-01

We have looked at the possible transverse instability effects which are caused by the deflecting modes of the rf cavities in PEP. The results are obtained by applying the expression of the instability damping rate. We have assumed that there equal bunches equally spaced in PEP. We have worked out the equivalent for a single bunch beam. The effect of chromaticity ξ is included as a frequency shift in the bunch mode spectra. We rewrite this result in terms of the transverse wake field instead of the impedance. We include an application of the Sacherer formalism to the case of resistive wall. The resulting expression of the damping rate contains two terms. The first term corresponds to the effect of the short wake fields; it agrees with the result of the head-tail instability as derived by Sands. A numerical estimate of this resistive-wall head tail case for PEP is given. It re-confirms that the resistive wall instability is not a serious problem for PEP. The second term gives the effect of long wake fields and it agrees with the result of Courant and Sessler. 10 refs., 2 figs

PEP radiation shielding tests in SLAC A Beam

International Nuclear Information System (INIS)

Ash, W.; DeStaebler, H.; Harris, J.; Jenkins, T.; Murray, J.

1977-09-01

Radiation shielding tests designed to simulate possible conditions in and around the PEP experimental halls were conducted. The SLAC A Beam was targeted in the block tunnel at a point about midway between End Station A and Beam Dump East. At that site it was relatively easy to rearrange the concrete block structure to simulate the various shielding configurations under consideration for PEP. Extensive surveys of neutron and ionizing radiation were made. Complete results of the shielding tests are given

Preschool Children's Performance on Profiling Elements of Prosody in Speech-Communication (PEPS-C)

Science.gov (United States)

Gibbon, Fiona E.; Smyth, Heather

2013-01-01

Profiling Elements of Prosody in Speech-Communication (PEPS-C) has not been used widely to assess prosodic abilities of preschool children. This study was therefore aimed at investigating typically developing 4-year-olds' performance on PEPS-C. PEPS-C was presented to 30 typically developing 4-year-olds recruited in southern Ireland. Children were…

KEKB and PEP-II B Factories

International Nuclear Information System (INIS)

Seeman, J.T.

1997-01-01

Two asymmetric B-Factories KEKB at KEK and PEP-II at SLAC are under construction, designed to study CP violation in the b-quark sector with a center of mass energy of 10.58 GeV. These two new accelerators are high luminosity two-ring two-energy e + e - colliders with one interaction point. There are many challenging accelerator physics and engineering issues associated with the high beam currents and high luminosities of these rings. The chosen solutions to these issues and the general parameters of the two rings are described in detail side-by-side. KEKB and PEP-II are well into the installation phase and are both scheduled to be completed in 1998. The particle physics programs are scheduled to start in 1999

Factors Associated With Forensic Nurses Offering HIV nPEP Status Post Sexual Assault.

Science.gov (United States)

Draughon, Jessica E; Hauda, William E; Price, Bonnie; Rotolo, Sue; Austin, Kim Wieczorek; Sheridan, Daniel J

2015-09-01

Nonoccupational, postexposure prophylaxis (nPEP) for human immunodeficiency virus (HIV) is offered inconsistently to patients who have been sexually assaulted. This may be due to Forensic Nurse Examiner (FNE) programs utilizing diverse nPEP protocols and HIV risk assessment algorithms. This study examines factors associated with FNEs offering nPEP to patients following sexual assault at two FNE programs in urban settings. Offering nPEP is mostly driven by site-specific protocol. At Site 1, in addition to open anal or open genital wounds, the presence of injury to the head or face was associated with FNEs offering nPEP (adjusted odds ratio [AOR] 64.15, 95% confidence interval [CI] = [2.12, 1942.37]). At Site 2, patients assaulted by someone of Other race/ethnicity (non-White, non-African American) were 86% less likely to be offered nPEP (AOR 0.14, 95% CI = [.03, .72]) than patients assaulted by Whites. In addition to following site-specific protocols, future research should further explore the mechanisms influencing clinician decision making. © The Author(s) 2014.

Isolation and characterization of PEP3, a gene required for vacuolar biogenesis in Saccharomyces cerevisiae.

OpenAIRE

Preston, R A; Manolson, M F; Becherer, K; Weidenhammer, E; Kirkpatrick, D; Wright, R; Jones, E W

1991-01-01

The Saccharomyces cerevisiae PEP3 gene was cloned from a wild-type genomic library by complementation of the carboxypeptidase Y deficiency in a pep3-12 strain. Subclone complementation results localized the PEP3 gene to a 3.8-kb DNA fragment. The DNA sequence of the fragment was determined; a 2,754-bp open reading frame predicts that the PEP3 gene product is a hydrophilic, 107-kDa protein that has no significant similarity to any known protein. The PEP3 predicted protein has a zinc finger (CX...

ARA-PEPs: a repository of putative sORF-encoded peptides in Arabidopsis thaliana.

Science.gov (United States)

Hazarika, Rashmi R; De Coninck, Barbara; Yamamoto, Lidia R; Martin, Laura R; Cammue, Bruno P A; van Noort, Vera

2017-01-17

Many eukaryotic RNAs have been considered non-coding as they only contain short open reading frames (sORFs). However, there is increasing evidence for the translation of these sORFs into bioactive peptides with potent signaling, antimicrobial, developmental, antioxidant roles etc. Yet only a few peptides encoded by sORFs are annotated in the model organism Arabidopsis thaliana. To aid the functional annotation of these peptides, we have developed ARA-PEPs (available at http://www.biw.kuleuven.be/CSB/ARA-PEPs ), a repository of putative peptides encoded by sORFs in the A. thaliana genome starting from in-house Tiling arrays, RNA-seq data and other publicly available datasets. ARA-PEPs currently lists 13,748 sORF-encoded peptides with transcriptional evidence. In addition to existing data, we have identified 100 novel transcriptionally active regions (TARs) that might encode 341 novel stress-induced peptides (SIPs). To aid in identification of bioactivity, we add functional annotation and sequence conservation to predicted peptides. To our knowledge, this is the largest repository of plant peptides encoded by sORFs with transcript evidence, publicly available and this resource will help scientists to effortlessly navigate the list of experimentally studied peptides, the experimental and computational evidence supporting the activity of these peptides and gain new perspectives for peptide discovery.

The Ustilago maydis effector Pep1 suppresses plant immunity by inhibition of host peroxidase activity.

Directory of Open Access Journals (Sweden)

Christoph Hemetsberger

Full Text Available The corn smut Ustilago maydis establishes a biotrophic interaction with its host plant maize. This interaction requires efficient suppression of plant immune responses, which is attributed to secreted effector proteins. Previously we identified Pep1 (Protein essential during penetration-1 as a secreted effector with an essential role for U. maydis virulence. pep1 deletion mutants induce strong defense responses leading to an early block in pathogenic development of the fungus. Using cytological and functional assays we show that Pep1 functions as an inhibitor of plant peroxidases. At sites of Δpep1 mutant penetrations, H₂O₂ strongly accumulated in the cell walls, coinciding with a transcriptional induction of the secreted maize peroxidase POX12. Pep1 protein effectively inhibited the peroxidase driven oxidative burst and thereby suppresses the early immune responses of maize. Moreover, Pep1 directly inhibits peroxidases in vitro in a concentration-dependent manner. Using fluorescence complementation assays, we observed a direct interaction of Pep1 and the maize peroxidase POX12 in vivo. Functional relevance of this interaction was demonstrated by partial complementation of the Δpep1 mutant defect by virus induced gene silencing of maize POX12. We conclude that Pep1 acts as a potent suppressor of early plant defenses by inhibition of peroxidase activity. Thus, it represents a novel strategy for establishing a biotrophic interaction.

An asymmetric Β-factory based on PEP

International Nuclear Information System (INIS)

Oddone, P.

1989-01-01

This paper reviews the motivation for building asymmetric B-factories based on storage rings and describes the current LBL/SLAC study to develop such a factory by modifying the PEP storage ring and adding a new low energy (2.5-3 GeV) storage ring. Collisions between the beam of the modified high energy PEP ring and the low energy ring lead to a luminosity of 2 x 10 33 cm- 2 sec - 1 at E cm = Y(4s) with currents of 0.8A in each beam. Further increases in current can lead to luminosities close to 10 34 cm - 2 sec - 1

New results on flavor production at PEP

International Nuclear Information System (INIS)

Barbaro-Galtieri, A.

1984-05-01

This report includes results from five PEP detectors: DELCO, HRS, MAC, MARK II and TPC. All, except the TPC, are presently taking data at PEP. The TPC is being upgraded: a new superconducting coil is being installed and other improvements are being implemented. The results discussed here are either new or improved since the Cornell Conference. New results on Particle Searches and a limit on neutrino generations are discussed. New data are included on weak couplings of c and b quarks. Various new results on hadron production are reported. All data were obtained in e +- collisions with total energy √s=29 GeV. 54 references

U.S. Ebola Treatment Center Clinical Laboratory Support.

Science.gov (United States)

Jelden, Katelyn C; Iwen, Peter C; Herstein, Jocelyn J; Biddinger, Paul D; Kraft, Colleen S; Saiman, Lisa; Smith, Philip W; Hewlett, Angela L; Gibbs, Shawn G; Lowe, John J

2016-04-01

Fifty-five hospitals in the United States have been designated Ebola treatment centers (ETCs) by their state and local health authorities. Designated ETCs must have appropriate plans to manage a patient with confirmed Ebola virus disease (EVD) for the full duration of illness and must have these plans assessed through a CDC site visit conducted by an interdisciplinary team of subject matter experts. This study determined the clinical laboratory capabilities of these ETCs. ETCs were electronically surveyed on clinical laboratory characteristics. Survey responses were returned from 47 ETCs (85%). Forty-one (87%) of the ETCs planned to provide some laboratory support (e.g., point-of-care [POC] testing) within the room of the isolated patient. Forty-four (94%) ETCs indicated that their hospital would also provide clinical laboratory support for patient care. Twenty-two (50%) of these ETC clinical laboratories had biosafety level 3 (BSL-3) containment. Of all respondents, 34 (72%) were supported by their jurisdictional public health laboratory (PHL), all of which had available BSL-3 laboratories. Overall, 40 of 44 (91%) ETCs reported BSL-3 laboratory support via their clinical laboratory and/or PHL. This survey provided a snapshot of the laboratory support for designated U.S. ETCs. ETCs have approached high-level isolation critical care with laboratory support in close proximity to the patient room and by distributing laboratory support among laboratory resources. Experts might review safety considerations for these laboratory testing/diagnostic activities that are novel in the context of biocontainment care. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Optical beam diagnostics on PEP

International Nuclear Information System (INIS)

Sabersky, A.P.

1981-02-01

In designing the PEP optical diagnostics we have been able to build on the experience gained with SPEAR. Most of the problems at SPEAR could be traced to the optical diagnostic system being inside the tunnel. A machine shutdown is required for any maintenance or modification. This implies that in order to make such an instrument successful, a large engineering effort must be mounted to ensure 100% operation at startup. The functions that do not work at startup may never be made to work; this has happened at several machines. Experimental setups are likewise risky and time consuming. A point which has been borne out in both SPEAR and PEP is that the mechanical part of the instrument, the special vacuum chamber, the optical mounts, the alignment and adjustments, require approximately 60% of the effort and cost of the optical diagnostics. It is far better to economize on detectors and electronics than on mechanical and optical essentials

DomPep--a general method for predicting modular domain-mediated protein-protein interactions.

Directory of Open Access Journals (Sweden)

Lei Li

Full Text Available Protein-protein interactions (PPIs are frequently mediated by the binding of a modular domain in one protein to a short, linear peptide motif in its partner. The advent of proteomic methods such as peptide and protein arrays has led to the accumulation of a wealth of interaction data for modular interaction domains. Although several computational programs have been developed to predict modular domain-mediated PPI events, they are often restricted to a given domain type. We describe DomPep, a method that can potentially be used to predict PPIs mediated by any modular domains. DomPep combines proteomic data with sequence information to achieve high accuracy and high coverage in PPI prediction. Proteomic binding data were employed to determine a simple yet novel parameter Ligand-Binding Similarity which, in turn, is used to calibrate Domain Sequence Identity and Position-Weighted-Matrix distance, two parameters that are used in constructing prediction models. Moreover, DomPep can be used to predict PPIs for both domains with experimental binding data and those without. Using the PDZ and SH2 domain families as test cases, we show that DomPep can predict PPIs with accuracies superior to existing methods. To evaluate DomPep as a discovery tool, we deployed DomPep to identify interactions mediated by three human PDZ domains. Subsequent in-solution binding assays validated the high accuracy of DomPep in predicting authentic PPIs at the proteome scale. Because DomPep makes use of only interaction data and the primary sequence of a domain, it can be readily expanded to include other types of modular domains.

The pepATTRACT web server for blind, large-scale peptide-protein docking.

Science.gov (United States)

de Vries, Sjoerd J; Rey, Julien; Schindler, Christina E M; Zacharias, Martin; Tuffery, Pierre

2017-07-03

Peptide-protein interactions are ubiquitous in the cell and form an important part of the interactome. Computational docking methods can complement experimental characterization of these complexes, but current protocols are not applicable on the proteome scale. pepATTRACT is a novel docking protocol that is fully blind, i.e. it does not require any information about the binding site. In various stages of its development, pepATTRACT has participated in CAPRI, making successful predictions for five out of seven protein-peptide targets. Its performance is similar or better than state-of-the-art local docking protocols that do require binding site information. Here we present a novel web server that carries out the rigid-body stage of pepATTRACT. On the peptiDB benchmark, the web server generates a correct model in the top 50 in 34% of the cases. Compared to the full pepATTRACT protocol, this leads to some loss of performance, but the computation time is reduced from ∼18 h to ∼10 min. Combined with the fact that it is fully blind, this makes the web server well-suited for large-scale in silico protein-peptide docking experiments. The rigid-body pepATTRACT server is freely available at http://bioserv.rpbs.univ-paris-diderot.fr/services/pepATTRACT. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

The PEP electron-positron ring

International Nuclear Information System (INIS)

Rees, J.R.

1988-01-01

The first stage of the positron-electron-proton (PEP) colliding-beam system which has been under joint study by a Lawrence Berkeley Laboratory-Stanford Linear Accelerator Center team for the past two years, will be the electron-positron storage ring. The physics justification for the e + e/sup minus/ ring is summarized briefly and the proposed facility is described. The ring will have six arcs having gross radii of about 220 m and six interaction regions located at the centers of straight sections about 130 m long. The longitudinal distance left free for experimental apparatus at the intersection regions will be 20 m. The range of operating beam energies will be from 5 GeV to 15 GeV. The design luminosity at 15 GeV will be 10 32 cm/sup minus 2/s/sup minus 1/, and the luminosity will vary approximately as the square of the beam energy. Alternative methods under consideration for adjusting the beam cross-section are discussed. The designs of the storage ring subsystems and of the conventional facilities including the experimental halls at the interaction regions are described

Influence of the metal ion on the enzyme activity and kinetics of PepA from Lactobacillus delbrueckii.

Science.gov (United States)

Ewert, Jacob; Glück, Claudia; Strasdeit, Henry; Fischer, Lutz; Stressler, Timo

2018-03-01

The aminopeptidase A (PepA; EC 3.4.11.7) belongs to the group of metallopeptidases with two bound metal ions per subunit (M1M2(PepA)) and is specific for the cleavage of N-terminal glutamic (Glu) and aspartic acid (Asp) and, in low amounts, serine (Ser) residues. Our group recently characterized the first PepA from a Lactobacillus strain. However, the characterization was performed using synthetic para-nitroaniline substrates and not original peptide substrates, as was done in the current study. Prior to the characterization using original peptide substrates, the PepA purified was converted to its inactive apo-form and eight different metal ions were tested to restore its activity. It was found that five of the metal ions were able to reactivate apo-PepA: Co 2+ , Cu 2+ , Mn 2+ , Ni 2+ and Zn 2+ . Interestingly, depending on the metal ion used for reactivation, the activity and the pH and temperature profile differed. Exemplarily, MnMn(PepA), NiNi(PepA) and ZnZn(PepA) had an activity optimum using MES buffer (50mM, pH 6.0) and 60°C, whereas the activity optimum changed to Na/K-phosphate-buffer (50mM, pH 7.0) and 55°C for CuCu(PepA). However, more important than the changes in optimum pH and temperature, the kinetic properties of PepA were affected by the metal ion used. While all PepA variants could release N-terminal Glu or Asp, only CoCo(PepA), NiNi(PepA) and CuCu(PepA) could release Ser from the particular peptide substrate. In addition, it was found that the enzyme efficiency (V max /K M ) and catalytic mechanism (positive cooperative binding (Hill coefficent; n), substrate inhibition (K IS )) were influenced by the metal ion. Exemplarily, a high cooperativity (n>2),K IS value >20mM and preference for N-terminal Glu were detected for CuCu(PepA). In summary, the results suggested that an exchange of the metal ion can be used for tailoring the properties of PepA for specific hydrolysis requirements. Copyright © 2017 Elsevier Inc. All rights reserved.

Pan European Phenological database (PEP725): a single point of access for European data

Science.gov (United States)

Templ, Barbara; Koch, Elisabeth; Bolmgren, Kjell; Ungersböck, Markus; Paul, Anita; Scheifinger, Helfried; Rutishauser, This; Busto, Montserrat; Chmielewski, Frank-M.; Hájková, Lenka; Hodzić, Sabina; Kaspar, Frank; Pietragalla, Barbara; Romero-Fresneda, Ramiro; Tolvanen, Anne; Vučetič, Višnja; Zimmermann, Kirsten; Zust, Ana

2018-02-01

The Pan European Phenology (PEP) project is a European infrastructure to promote and facilitate phenological research, education, and environmental monitoring. The main objective is to maintain and develop a Pan European Phenological database (PEP725) with an open, unrestricted data access for science and education. PEP725 is the successor of the database developed through the COST action 725 "Establishing a European phenological data platform for climatological applications" working as a single access point for European-wide plant phenological data. So far, 32 European meteorological services and project partners from across Europe have joined and supplied data collected by volunteers from 1868 to the present for the PEP725 database. Most of the partners actively provide data on a regular basis. The database presently holds almost 12 million records, about 46 growing stages and 265 plant species (including cultivars), and can be accessed via http://www.pep725.eu/. Users of the PEP725 database have studied a diversity of topics ranging from climate change impact, plant physiological question, phenological modeling, and remote sensing of vegetation to ecosystem productivity.

Pan European Phenological database (PEP725): a single point of access for European data

Science.gov (United States)

Templ, Barbara; Koch, Elisabeth; Bolmgren, Kjell; Ungersböck, Markus; Paul, Anita; Scheifinger, Helfried; Rutishauser, This; Busto, Montserrat; Chmielewski, Frank-M.; Hájková, Lenka; Hodzić, Sabina; Kaspar, Frank; Pietragalla, Barbara; Romero-Fresneda, Ramiro; Tolvanen, Anne; Vučetič, Višnja; Zimmermann, Kirsten; Zust, Ana

2018-06-01

The Pan European Phenology (PEP) project is a European infrastructure to promote and facilitate phenological research, education, and environmental monitoring. The main objective is to maintain and develop a Pan European Phenological database (PEP725) with an open, unrestricted data access for science and education. PEP725 is the successor of the database developed through the COST action 725 "Establishing a European phenological data platform for climatological applications" working as a single access point for European-wide plant phenological data. So far, 32 European meteorological services and project partners from across Europe have joined and supplied data collected by volunteers from 1868 to the present for the PEP725 database. Most of the partners actively provide data on a regular basis. The database presently holds almost 12 million records, about 46 growing stages and 265 plant species (including cultivars), and can be accessed via http://www.pep725.eu/ . Users of the PEP725 database have studied a diversity of topics ranging from climate change impact, plant physiological question, phenological modeling, and remote sensing of vegetation to ecosystem productivity.

PEP magnet power supply systems

International Nuclear Information System (INIS)

Jackson, L.T.

1977-01-01

The dc electrical requirements of the PEP magnets fall mainly into two categories: (1) high power and current of single polarity and (2) low-power bi-polar. The first category will be thyristor-chopper controlled off common 600 V dc busses. The second group will utilize continuously controlled push-pull transistor actuators

Pep2Path: automated mass spectrometry-guided genome mining of peptidic natural products.

Directory of Open Access Journals (Sweden)

Marnix H Medema

2014-09-01

Full Text Available Nonribosomally and ribosomally synthesized bioactive peptides constitute a source of molecules of great biomedical importance, including antibiotics such as penicillin, immunosuppressants such as cyclosporine, and cytostatics such as bleomycin. Recently, an innovative mass-spectrometry-based strategy, peptidogenomics, has been pioneered to effectively mine microbial strains for novel peptidic metabolites. Even though mass-spectrometric peptide detection can be performed quite fast, true high-throughput natural product discovery approaches have still been limited by the inability to rapidly match the identified tandem mass spectra to the gene clusters responsible for the biosynthesis of the corresponding compounds. With Pep2Path, we introduce a software package to fully automate the peptidogenomics approach through the rapid Bayesian probabilistic matching of mass spectra to their corresponding biosynthetic gene clusters. Detailed benchmarking of the method shows that the approach is powerful enough to correctly identify gene clusters even in data sets that consist of hundreds of genomes, which also makes it possible to match compounds from unsequenced organisms to closely related biosynthetic gene clusters in other genomes. Applying Pep2Path to a data set of compounds without known biosynthesis routes, we were able to identify candidate gene clusters for the biosynthesis of five important compounds. Notably, one of these clusters was detected in a genome from a different subphylum of Proteobacteria than that in which the molecule had first been identified. All in all, our approach paves the way towards high-throughput discovery of novel peptidic natural products. Pep2Path is freely available from http://pep2path.sourceforge.net/, implemented in Python, licensed under the GNU General Public License v3 and supported on MS Windows, Linux and Mac OS X.

Experiences of HIV postexposure prophylaxis (PEP) among highly exposed men who have sex with men (MSM).

Science.gov (United States)

Palich, Romain; Martin-Blondel, Guillaume; Cuzin, Lise; Le Talec, Jean-Yves; Boyer, Pierre; Massip, Patrice; Delobel, Pierre

2017-11-01

HIV postexposure prophylaxis (PEP) is indicated after sexual exposure with high risk of transmission. Men who have sex with men (MSM) are the main target of PEP. The aim of our study was to investigate the experience and shortcomings of PEP among people with a high risk of HIV exposure. Subjects with ongoing follow-up for HIV infection and PEP history were selected for the qualitative study. Semistructured interviews were conducted at the patients' homes. They were audio-recorded, transcribed and deidentified before data analysis, double coding and thematic analysis with an inductive approach. Twenty-three patients were eligible for the qualitative study. Thirteen interviews were carried out. All patients were 20-60-year-old MSM. The median time between PEP and HIV diagnosis was 3.3 years (interquartile range (IQR) 25-75 =0.9-4.9). Many participants reported negative PEP experiences: awkward access to the PEP clinic, uneasiness and shame in the hospital setting, unpleasant interaction and moral disapprobation from the medical staff, treatment intolerance and prevention messages that were 'inconsistent with real life' CONCLUSION: Our data highlight PEP management failures among its target population that may have compromised any subsequent attempts to seek out PEP. Practitioners should be more aware of MSM sexual contexts and practices. PEP consultations should provide the opportunity to discuss prevention strategies with highly exposed HIV-negative subjects, which may include pre-exposure prophylaxis. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

PEP-II design update and R ampersand D results

International Nuclear Information System (INIS)

Zisman, M.S.

1993-01-01

We describe the present status of the PEP-II asymmetric β factory design undertaken by SLAC, LBL, and LLNL. Design optimization during the past year and changes from the original CDR design are described. R ampersand D activities have focused primarily on the key technology areas of vacuum, RF, and feedback system design. Recent progress in these areas is described. The R ampersand D results have verified our design assumptions and provide further confidence in the design of PEP-II

Recent improvements in luminosity at PEP

International Nuclear Information System (INIS)

Helm, R.; Allen, M.; Chao, A.

1983-03-01

We will describe improvements which have led to new records for peak and average luminosity at PEP. Comparison of recent results with several earlier lattice and optical modifications shows rather good correlation with the predictions of a beam-beam simulation program

Ca 2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca 2+ channels

KAUST Repository

Qia, Zhi; Verma, Rajeev K.; Gehring, Christoph A; Yamaguchi, Yube; Zhao, Yichen; Ryan, Clarence A.; Berkowitz, Gerald A.

2010-01-01

receptor- like kinase receptor AtPepR1 has guanylyl cyclase activity, generating cGMP from GTP, and that cGMP can activate CNGC2- dependent cytosolic Ca 2+ elevation. AtPep-dependent expression of pathogen-defense genes (PDF1.2, MPK3, and WRKY33

PEP cooling water systems and underground piped utilities design criteria report

International Nuclear Information System (INIS)

Hall, F.; Robbins, D.

1975-10-01

This paper discusses the cooling systems required by the PEP Storage Ring. Particular topics discussed are: Cooling tower systems, RF cavity and vacuum chamber LCW cooling systems, klystron and ring magnet LLW cooling systems, Injection magnet LCW Cooling Systems; PEP interaction area detector LCW Cooling Systems; and underground piped utilities. 1 ref., 20 figs

An Over-damped Cavity Longitudinal Kicker for the PEP-II LER

CERN Document Server

McIntosh, P

2003-01-01

Both rings of PEP-II use drift tube kickers in the longitudinal bunch-by-bunch feedback system. Efforts are now underway to increase the stored beam currents and luminosity of PEP-II, and beam-induced heating of these structures, particularly in the Low Energy Ring (LER) is of concern. An alternative kicker design based on the over-damped cavity kicker, first developed by INFN-Frascati is being built for PEP-II. This low loaded Q (or wide bandwidth) structure is fed by a network of ridged waveguides coupled to a simple pill-box cavity. Beam induced RF power is also coupled out of the cavity to external loads, so that the higher order modes (HOMs) excited in the structure are well-damped. This paper details the kicker design for PEP-II and discusses some of the design trade-offs between shunt impedance and bandwidth, as well as the influence of the feedthroughs on the kicker parameters. Estimates of the expected power deposition in the cavity are also provided.

Nuclear physics at PEP: First test and future plans

International Nuclear Information System (INIS)

Van Bibber, K.; Dietrich, F.S.; Melnikoff, S.O.

1986-09-01

A test run of internal target nuclear physics at the PEP storage ring is described. The Time Projection Chamber (TPC-2γ detector) was used to detect the inelastically scattered electron and complete hadronic final state in the interaction of 14.5 GeV electrons with D 2 , Ar and Xe gas targets. The data comprise mostly low-x low-Q 2 events, but some deep inelastic scattering as well. The future possibilities of a dedicated nuclear physics program at PEP are outlined. 15 refs., 25 figs

Event rates to be expected at PEP

International Nuclear Information System (INIS)

Richter, B.

1988-01-01

As a guide for planning experiments at PEP, some estimates are given of average luminosity, charged and neutral particle yields vs. momentum, QED test capabilities, and weak interaction effects. 2 figs

Plans for PEP survey and alignment: Status report

International Nuclear Information System (INIS)

Gunn, J.; Sah, R.

1975-01-01

This note discusses the current state of survey and alignment plans for PEP Stage I. Several surveying techniques are described; one is described in considerable detail. The survey and alignment task for PEP consists of positioning approximately 700 ring magnets, 100 injection line magnets, and 100 miscellaneous components. The alignment tolerances are tight and they are set by the requirement that closed orbit distortions must be small for proper storage ring operation. The alignment problem is aggravated by the circumstance that the storage ring components are placed in tunnels which span a large area (over 700 meters across) and which do not permit long lines of sight. 6 ref., 1 fig

Problems in obtaining polarized e+ and e- beams and perspectives for PEP

International Nuclear Information System (INIS)

Chao, A.W.

1980-09-01

A matrix formalism for polarization calculation, as well as its comparison with other methods, is briefly discussed. The prediction for SPEAR is compared with experimental measurements. An estimate is offered for the transverse polarization for PEP. Various schemes for obtaining the longitudinal polarization in PEP are studied

Pep-3D-Search: a method for B-cell epitope prediction based on mimotope analysis.

Science.gov (United States)

Huang, Yan Xin; Bao, Yong Li; Guo, Shu Yan; Wang, Yan; Zhou, Chun Guang; Li, Yu Xin

2008-12-16

The prediction of conformational B-cell epitopes is one of the most important goals in immunoinformatics. The solution to this problem, even if approximate, would help in designing experiments to precisely map the residues of interaction between an antigen and an antibody. Consequently, this area of research has received considerable attention from immunologists, structural biologists and computational biologists. Phage-displayed random peptide libraries are powerful tools used to obtain mimotopes that are selected by binding to a given monoclonal antibody (mAb) in a similar way to the native epitope. These mimotopes can be considered as functional epitope mimics. Mimotope analysis based methods can predict not only linear but also conformational epitopes and this has been the focus of much research in recent years. Though some algorithms based on mimotope analysis have been proposed, the precise localization of the interaction site mimicked by the mimotopes is still a challenging task. In this study, we propose a method for B-cell epitope prediction based on mimotope analysis called Pep-3D-Search. Given the 3D structure of an antigen and a set of mimotopes (or a motif sequence derived from the set of mimotopes), Pep-3D-Search can be used in two modes: mimotope or motif. To evaluate the performance of Pep-3D-Search to predict epitopes from a set of mimotopes, 10 epitopes defined by crystallography were compared with the predicted results from a Pep-3D-Search: the average Matthews correlation coefficient (MCC), sensitivity and precision were 0.1758, 0.3642 and 0.6948. Compared with other available prediction algorithms, Pep-3D-Search showed comparable MCC, specificity and precision, and could provide novel, rational results. To verify the capability of Pep-3D-Search to align a motif sequence to a 3D structure for predicting epitopes, 6 test cases were used. The predictive performance of Pep-3D-Search was demonstrated to be superior to that of other similar programs

The key determinants of perceived external prestige (PEP – Qualitative research approach

Directory of Open Access Journals (Sweden)

Tamara Sušanj Šulentić

2017-01-01

Full Text Available Perceived external prestige (PEP is a well-known concept oriented towards describing the way members of a certain organization interpret and assess their organisational reputation. Such perception can significantly affect employees’ identification and loyalty to the organisation as well as their job satisfaction and work performance. According to the social identification theory, people define themselves and others with respect to their belonging to a particular group or organization, and their basic motive is a personal need for self-respect and a sense of pride. Employees’ perception of being members of an important, reputable and significant organisation contributes to the feeling of self-respect, which increases their individual social value and status. According to literature, there are only few objections to the concept of PEP, mainly those related to the determination of its structure and, with it, to its management. Although there is a growing literature on PEP, it is still not clear whether PEP is a one-dimensional or a multidimensional construct. If PEP is a multidimensional concept, it is important to specify its key components, in order to enhance the management of favourable organizational prestige in a real work environment. The purpose of this paper was to determine the structure of PEP and important sources of information, based on which, employees value the prestige of their organisation. Qualitative research was conducted, comprising nine semi-structured interviews with communication experts working for a multinational organisation operating in several European countries. The results of this paper indicate that PEP is a multidimensional construct, with several components, important for its creation and management. Those components can be grouped into three main categories: track record of success and the position of the organisation on the market, social impact of the organisation on the immediate environment and the

Stanford Synchrotron Radiation Laboratory activity report for 1987

Energy Technology Data Exchange (ETDEWEB)

Robinson, S.; Cantwell, K. [eds.

1988-12-31

During 1987, SSRL achieved many significant advances and reached several major milestones utilizing both SPEAR and PEP as synchrotron radiation sources as described in this report. Perhaps the following two are worthy of particular mention: (1) SPEAR reached an all time high of 4,190 delivered user-shifts during calendar year 1987, highlights of the many scientific results are given; (2) during a 12 day run in December of 1987, PEP was operated in a low emittance mode (calculated emittance 6.4 nanometer-radians) at 7.1 GeV with currents up to 33 mA. A second undulator beam line on PEP was commissioned during this run and used to record many spectra showing the extremely high brightness of the radiation. PEP is now by far the highest brightness synchrotron radiation source in the world. The report is divided into the following sections: (1) laboratory operations; (2) accelerator physics programs; (3) experimental facilities; (4) engineering division; (5) conferences and workshops; (6) SSRL organization; (7) experimental progress reports; (8) active proposals; (9) SSRL experiments and proposals by institution; and (10) SSRL publications.

Evidence that the rabbit proton-peptide co-transporter PepT1 is a multimer when expressed in Xenopus laevis oocytes.

Science.gov (United States)

Panitsas, Konstantinos-E; Boyd, C A R; Meredith, David

2006-04-01

To test whether the rabbit proton-coupled peptide transporter PepT1 is a multimer, we have employed a combination of transport assays, luminometry and site-directed mutagenesis. A functional epitope-tagged PepT1 construct (PepT1-FLAG) was co-expressed in Xenopus laevis oocytes with a non-functional but normally trafficked mutant form of the same transporter (W294F-PepT1). The amount of PepT1-FLAG cRNA injected into the oocytes was kept constant, while the amount of W294F-PepT1 cRNA was increased over the mole fraction range of 0 to 1. The uptake of [(3)H]-D: -Phe-L: -Gln into the oocytes was measured at pH(out) 5.5, and the surface expression of PepT1-FLAG was quantified by luminometry. As the mole fraction of injected W294F-PepT1 increased, the uptake of D: -Phe-L: -Gln decreased. This occurred despite the surface expression of PepT1-FLAG remaining constant, and so we can conclude that PepT1 must be a multimer. Assuming that PepT1 acts as a homomultimer, the best fit for the modelling suggests that PepT1 could be a tetramer, with a minimum requirement of two functional subunits in each protein complex. Western blotting also showed the presence of higher-order complexes of PepT1-FLAG in oocyte membranes. It should be noted that we cannot formally exclude the possibility that PepT1 interacts with unidentified Xenopus protein(s). The finding that PepT1 is a multimer has important implications for the molecular modelling of this protein.

Is there an optimal level of positive expiratory pressure (PEP) to improve walking tolerance in patients with severe COPD?

Science.gov (United States)

Russo, Davide; Simonelli, Carla; Paneroni, Mara; Saleri, Manuela; Piroddi, Ines Maria Grazia; Cardinale, Francesco; Vitacca, Michele; Nicolini, Antonello

2016-07-01

The application of positive expiratory pressure (PEP) devices during exercise had been proposed in order to counteract the pulmonary hyperinflation, reduce the dyspnea and thus increase the exercise tolerance in patients with severe chronic obstructive pulmonary disease (COPD). This randomized controlled crossover trial investigated the effect of two different levels of PEP (1 cmH2O and 10 cmH2O) on distance covered at 6minute walk test (6MWT) in patients with severe COPD. Secondary outcomes were the evaluation of PEP effects on physiological and pulmonary function variables. Seventy-two severe COPD patients, referred to our hospitals as in and out patients, were recruited. A basal 6MWT without devices was performed on the first day, and then repeated with PEP 1 cmH2O (PEP1) and 10 cmH2O (PEP10), with a randomized crossover design. Slow and forced spirometries, including the inspiratory capacity measure, were repeated before and after each 6MWT. 50 patients (average age 69,92 year, mean FEV1 41,42% of predicted) concluded the trial. The 6MWT improved significantly among both PEP levels and baseline (323,8 mt at baseline vs. 337,8 PEP1 and 341,8 PEP10; p<.002 and p<.018, respectively). The difference between PEP10 and PEP1 did not reach the significance. No improvements were found in pulmonary function, symptoms and physiological variables after the 6MWT. In patients with severe COPD, the application of 1 cmH2O of PEP seems to improve the exercise tolerance as 10 cmH2O, with similar dyspnea. Further studies should investigate the effects of low levels of PEP on aerobic training programs. Copyright © 2016 SEPAR. Published by Elsevier Espana. All rights reserved.

The First Year of the BABAR Experiment at PEP-II

Energy Technology Data Exchange (ETDEWEB)

Barrera, Barbara

2000-12-18

The BABAR detector, situated at the SLAC PEP-II asymmetric e{sup +}e{sup -} collider, has been recording data at energies on and around the {Upsilon}(4S) resonance since May 1999. In this paper, we briefly describe the PEP-II B Factory and the BABAR detector. The performance presently achieved by the experiment in the areas of tracking, vertexing, calorimetry and particle identification is reviewed. Analysis concepts that are used in the various papers submitted to this conference are also discussed.

Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling

KAUST Repository

Lori, M.

2015-05-22

Plant elicitor peptides (Peps) are potent inducers of pattern-triggered immunity and amplify the immune response against diverse pathogens. Peps have been discovered and studied extensively in Arabidopsis and only recently orthologs in maize were also identified and characterized in more detail. Here, the presence of PROPEPs, the Pep precursors, and PEPRs, the Pep receptors, was investigated within the plant kingdom. PROPEPs and PEPRs were identified in most sequenced species of the angiosperms. The conservation and compatibility of the Pep-PEPR-system was analysed by using plants of two distantly related dicot families, Brassicaceae and Solanaceae, and a representative family of monocot plants, the Poaceae. All three plant families contain important crop plants, including maize, rice, tomato, potato, and canola. Peps were not recognized by species outside of their plant family of origin, apparently because of a divergence of the Pep sequences. Three family-specific Pep motifs were defined and the integration of such a motif into the Pep sequence of an unrelated Pep enabled its perception. Transient transformation of Nicotiana benthamiana with the coding sequences of the AtPEPR1 and ZmPEPR1a led to the recognition of Pep peptides of Brassicaceae or Poaceae origin, respectively, and to the proper activation of downstream signalling. It was concluded that signalling machinery downstream of the PEPRs is highly conserved whereas the leucine-rich repeat domains of the PEPRs co-evolved with the Peps, leading to distinct motifs and, with it, interfamily incompatibility.

Aircraft Wiring Support Equipment Integration Laboratory (AWSEIL)

Data.gov (United States)

Federal Laboratory Consortium — Purpose:The Aircraft Wiring Support Equipment Integration Laboratory (AWSEIL) provides a variety of research, design engineering and prototype fabrication services...

Perception of Arabidopsis AtPep peptides, but not bacterial elicitors, accelerates starvation-induced senescence

Directory of Open Access Journals (Sweden)

Kay eGully

2015-01-01

Full Text Available Members of the AtPep group of Arabidopsis endogenous peptides have frequently been reported to induce pattern-triggered immunity and to increase resistance to diverse pathogens by amplifying the innate immune response. Here, we made the surprising observation that dark-induced leaf senescence was accelerated by the presence of Peps. Adult leaves as well as leaf discs of Col-0 wild type plants showed a Pep-triggered early onset of chlorophyll breakdown and leaf yellowing whereas pepr1 pepr2 double mutant plants were insensitive. In addition, this response was dependent on ethylene signaling and inhibited by the addition of cytokinins. Notably, addition of the bacterial elicitors flg22 or elf18, both potent inducers of pattern-triggered immunity, did not provoke an early onset of leaf senescence.Continuous darkness leads to energy deprivation and starvation and therewith promotes leaf senescence. We found that continuous darkness also strongly induced PROPEP3 transcription. Moreover, Pep-perception led to a rapid induction of PAO, APG7 and APG8a, genes indispensable for chlorophyll degradation as well as autophagy, respectively, and all three hallmarks of starvation and senescence. Notably, addition of sucrose as a source of energy inhibited the Pep-triggered early onset of senescence. In conclusion, we report that Pep-perception accelerates dark/starvation-induced senescence via an early induction of chlorophyll degradation and autophagy. This represents a novel and unique characteristic of PEPR signaling, unrelated to pattern-triggered immunity.

An operon from Lactobacillus helveticus composed of a proline iminopeptidase gene (pepI) and two genes coding for putative members of the ABC transporter family of proteins.

Science.gov (United States)

Varmanen, P; Rantanen, T; Palva, A

1996-12-01

A proline iminopeptidase gene (pepI) of an industrial Lactobacillus helveticus strain was cloned and found to be organized in an operon-like structure of three open reading frames (ORF1, ORF2 and ORF3). ORF1 was preceded by a typical prokaryotic promoter region, and a putative transcription terminator was found downstream of ORF3, identified as the pepI gene. Using primer-extension analyses, only one transcription start site, upstream of ORF1, was identifiable in the predicted operon. Although the size of mRNA could not be judged by Northern analysis either with ORF1-, ORF2- or pepI-specific probes, reverse transcription-PCR analyses further supported the operon structure of the three genes. ORF1, ORF2 and ORF3 had coding capacities for 50.7, 24.5 and 33.8 kDa proteins, respectively. The ORF3-encoded PepI protein showed 65% identity with the PepI proteins from Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus delbrueckii subsp. lactis. The ORF1-encoded protein had significant homology with several members of the ABC transporter family but, with two distinct putative ATP-binding sites, it would represent an unusual type among the bacterial ABC transporters. ORF2 encoded a putative integral membrane protein also characteristic of the ABC transporter family. The pepI gene was overexpressed in Escherichia coli. Purified PepI hydrolysed only di and tripeptides with proline in the first position. Optimum PepI activity was observed at pH 7.5 and 40 degrees C. A gel filtration analysis indicated that PepI is a dimer of M(r) 53,000. PepI was shown to be a metal-independent serine peptidase having thiol groups at or near the active site. Kinetic studies with proline-p-nitroanilide as substrate revealed Km and Vmax values of 0.8 mM and 350 mmol min-1 mg-1, respectively, and a very high turnover number of 135,000 s-1.

Evaluation of the BEAM--BEAM effect in PEP using Myer's simulation program

International Nuclear Information System (INIS)

Hutton, A.

1982-09-01

The program BEAM BEAM written by Steve Myers for the LEP machine at CERN has given encouraging results in the simulation of the beam-beam effect in electron-positron storage rings. It therefore seemed worthwhile to apply the program to PEP with two main intentions. Firstly, to confirm the validity of the program by comparison with experimental data from previous PEP runs and secondly, to search for an improvement in the operating conditions of PEP. Clearly a successful prediction would also enhance the credibility of the program. The program itself has been extensively described in the literature and will not be repeated here, except for some comments of direct relevance to the present simulation. 14 refs., 15 figs., 4 tabs

Fleet Aviation Maintenance Organic Support (FAMOS) Laboratory

Data.gov (United States)

Federal Laboratory Consortium — Purpose:The Fleet Aviation Maintenance Organic Support (FAMOS) Laboratory at the Naval Air Warfare Center Aircraft Division, Lakehurst, NJ provides rapid engineering...

Five-year cost-effectiveness of the Patient Empowerment Programme (PEP) for type 2 diabetes mellitus in primary care.

Science.gov (United States)

Lian, Jinxiao; McGhee, Sarah M; So, Ching; Chau, June; Wong, Carlos K H; Wong, William C W; Lam, Cindy L K

2017-09-01

This study evaluated the short-term cost-effectiveness of the Patient Empowerment Programme (PEP) for diabetes mellitus (DM) in Hong Kong. Propensity score matching was used to select a matched group of PEP and non-PEP subjects. A societal perspective was adopted to estimate the cost of PEP. Outcome measures were the cumulative incidence of all-cause mortality and diabetic complication over a 5-year follow-up period and the number needed to treat (NNT) to avoid 1 event. The incremental cost-effectiveness ratio (ICER) of cost per event avoided was calculated using the PEP cost per subject multiplied by the NNT. The PEP cost per subject from the societal perspective was US$247. There was a significantly lower cumulative incidence of all-cause mortality (2.9% vs 4.6%, P US$14 465, US$19 617 and US$30 796, respectively. The extra amount allocated to managing PEP was small and it appears cost-effective in the short-term as an addition to RAMP. © 2017 John Wiley & Sons Ltd.

A new lattice for PEP

International Nuclear Information System (INIS)

Rees, J.; Wiedemann, H.

1976-01-01

A new low-beta configuration has been proposed for PEP which has a reduced β/sub y/* and is capable of delivering design luminosity with lower circulating beam currents that those required in the standard configuration described in the Conceptual Design Report of February, 1976. A feasibility study has been carried out and it is reported in PTM-65, September 8, 1976, by Lee, Morton and Wiedemann. The beam-stay-clear region is specified in PTM-66, September 3, 1976 by H. Wiedemann. The principal advantages of the new lattice are that the lower beam currents lead to lower higher-order-mode power and permit reduction of the total rf power required which in turn reduces both capital cost and operating cost. Its disadvantages stem primarily from the relatively high maximum beta values which occur in the interaction-region quadrupoles. These produce high chromaticities which require a generally stronger sextupole system for their compensation. The advantages outweigh the disadvantages and the new 11-cm-beta configuration has been adopted as the design configuration for energies of 15-GeV and lower. In the remainder of this report we shall discuss the implications of this new configuration for the various systems of PEP. 1 fig., 3 tabs

PEP-II status report

International Nuclear Information System (INIS)

Dorfan, J.M.

1998-06-01

The main design features of the PEP-II asymmetric two-ring electron-positron B Factory collider, built at the Stanford Linear Accelerator Center, are described. This facility will complete construction in June 1998. The high energy ring, completed in May 1997, has had 3 months of commissioning and successfully stored 0.75 A of electrons. The success of the high energy ring testing validates not only its ring components, but also the injection system, the RF system and the control system all of which are common to the two rings

Transduction of PEP-1-heme oxygenase-1 into insulin-producing INS-1 cells protects them against cytokine-induced cell death

Energy Technology Data Exchange (ETDEWEB)

Lee, Su Jin; Kang, Hyung Kyung [Department of Physiology, College of Medicine, Hallym University, Chunchon 200-702 (Korea, Republic of); Song, Dong Keun [Department of Pharmacology, College of Medicine, Hallym University, Chunchon 200-702 (Korea, Republic of); Eum, Won Sik; Park, Jinseu [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Choi, Soo Young, E-mail: sychoi@hallym.ac.kr [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Kwon, Hyeok Yil, E-mail: hykwon@hallym.ac.kr [Department of Physiology, College of Medicine, Hallym University, Chunchon 200-702 (Korea, Republic of)

2015-06-05

Pro-inflammatory cytokines play a crucial role in the destruction of pancreatic β-cells, thereby triggering the development of autoimmune diabetes mellitus. We recently developed a cell-permeable fusion protein, PEP-1-heme oxygenase-1 (PEP-1-HO-1) and investigated the anti-inflammatory effects in macrophage cells. In this study, we transduced PEP-1-HO-1 into INS-1 insulinoma cells and examined its protective effect against cytokine-induced cell death. PEP-1-HO-1 was successfully delivered into INS-1 cells in time- and dose-dependent manner and was maintained within the cells for at least 48 h. Pre-treatment with PEP-1-HO-1 increased the survival of INS-1 cells exposed to cytokine mixture (IL-1β, IFN-γ, and TNF-α) in a dose-dependent manner. PEP-1-HO-1 markedly decreased cytokine-induced production of reactive oxygen species (ROS), nitric oxide (NO), and malondialdehyde (MDA). These protective effects of PEP-1-HO-1 against cytokines were correlated with the changes in the levels of signaling mediators of inflammation (iNOS and COX-2) and cell apoptosis/survival (Bcl-2, Bax, caspase-3, PARP, JNK, and Akt). These results showed that the transduced PEP-1-HO-1 efficiently prevented cytokine-induced cell death of INS-1 cells by alleviating oxidative/nitrosative stresses and inflammation. Further, these results suggested that PEP-1-mediated HO-1 transduction may be a potential therapeutic strategy to prevent β-cell destruction in patients with autoimmune diabetes mellitus. - Highlights: • We showed that PEP-1-HO-1 was efficiently delivered into INS-1 cells. • Transduced PEP-1-HO-1 exerted a protective effect against cytokine-induced cell death. • Transduced PEP-1-HO-1 inhibited cytokine-induced ROS and NO accumulation. • PEP-1-HO-1 suppressed cytokine-induced expression of iNOS, COX-2, and Bax. • PEP-1-HO-1 transduction may be an efficient tool to prevent β-cell destruction.

Transduction of PEP-1-heme oxygenase-1 into insulin-producing INS-1 cells protects them against cytokine-induced cell death

International Nuclear Information System (INIS)

Lee, Su Jin; Kang, Hyung Kyung; Song, Dong Keun; Eum, Won Sik; Park, Jinseu; Choi, Soo Young; Kwon, Hyeok Yil

2015-01-01

Pro-inflammatory cytokines play a crucial role in the destruction of pancreatic β-cells, thereby triggering the development of autoimmune diabetes mellitus. We recently developed a cell-permeable fusion protein, PEP-1-heme oxygenase-1 (PEP-1-HO-1) and investigated the anti-inflammatory effects in macrophage cells. In this study, we transduced PEP-1-HO-1 into INS-1 insulinoma cells and examined its protective effect against cytokine-induced cell death. PEP-1-HO-1 was successfully delivered into INS-1 cells in time- and dose-dependent manner and was maintained within the cells for at least 48 h. Pre-treatment with PEP-1-HO-1 increased the survival of INS-1 cells exposed to cytokine mixture (IL-1β, IFN-γ, and TNF-α) in a dose-dependent manner. PEP-1-HO-1 markedly decreased cytokine-induced production of reactive oxygen species (ROS), nitric oxide (NO), and malondialdehyde (MDA). These protective effects of PEP-1-HO-1 against cytokines were correlated with the changes in the levels of signaling mediators of inflammation (iNOS and COX-2) and cell apoptosis/survival (Bcl-2, Bax, caspase-3, PARP, JNK, and Akt). These results showed that the transduced PEP-1-HO-1 efficiently prevented cytokine-induced cell death of INS-1 cells by alleviating oxidative/nitrosative stresses and inflammation. Further, these results suggested that PEP-1-mediated HO-1 transduction may be a potential therapeutic strategy to prevent β-cell destruction in patients with autoimmune diabetes mellitus. - Highlights: • We showed that PEP-1-HO-1 was efficiently delivered into INS-1 cells. • Transduced PEP-1-HO-1 exerted a protective effect against cytokine-induced cell death. • Transduced PEP-1-HO-1 inhibited cytokine-induced ROS and NO accumulation. • PEP-1-HO-1 suppressed cytokine-induced expression of iNOS, COX-2, and Bax. • PEP-1-HO-1 transduction may be an efficient tool to prevent β-cell destruction

PepMapper: a collaborative web tool for mapping epitopes from affinity-selected peptides.

Directory of Open Access Journals (Sweden)

Wenhan Chen

Full Text Available Epitope mapping from affinity-selected peptides has become popular in epitope prediction, and correspondingly many Web-based tools have been developed in recent years. However, the performance of these tools varies in different circumstances. To address this problem, we employed an ensemble approach to incorporate two popular Web tools, MimoPro and Pep-3D-Search, together for taking advantages offered by both methods so as to give users more options for their specific purposes of epitope-peptide mapping. The combined operation of Union finds as many associated peptides as possible from both methods, which increases sensitivity in finding potential epitopic regions on a given antigen surface. The combined operation of Intersection achieves to some extent the mutual verification by the two methods and hence increases the likelihood of locating the genuine epitopic region on a given antigen in relation to the interacting peptides. The Consistency between Intersection and Union is an indirect sufficient condition to assess the likelihood of successful peptide-epitope mapping. On average from 27 tests, the combined operations of PepMapper outperformed either MimoPro or Pep-3D-Search alone. Therefore, PepMapper is another multipurpose mapping tool for epitope prediction from affinity-selected peptides. The Web server can be freely accessed at: http://informatics.nenu.edu.cn/PepMapper/

First Evidence of pep Solar Neutrinos by Direct Detection in Borexino

Science.gov (United States)

Bellini, G.; Benziger, J.; Bick, D.; Bonetti, S.; Bonfini, G.; Bravo, D.; Buizza Avanzini, M.; Caccianiga, B.; Cadonati, L.; Calaprice, F.; Carraro, C.; Cavalcante, P.; Chavarria, A.; Chepurnov, A.; D'Angelo, D.; Davini, S.; Derbin, A.; Etenko, A.; Fomenko, K.; Franco, D.; Galbiati, C.; Gazzana, S.; Ghiano, C.; Giammarchi, M.; Goeger-Neff, M.; Goretti, A.; Grandi, L.; Guardincerri, E.; Hardy, S.; Ianni, Aldo; Ianni, Andrea; Korablev, D.; Korga, G.; Koshio, Y.; Kryn, D.; Laubenstein, M.; Lewke, T.; Litvinovich, E.; Loer, B.; Lombardi, F.; Lombardi, P.; Ludhova, L.; Machulin, I.; Manecki, S.; Maneschg, W.; Manuzio, G.; Meindl, Q.; Meroni, E.; Miramonti, L.; Misiaszek, M.; Montanari, D.; Mosteiro, P.; Muratova, V.; Oberauer, L.; Obolensky, M.; Ortica, F.; Otis, K.; Pallavicini, M.; Papp, L.; Perasso, L.; Perasso, S.; Pocar, A.; Quirk, J.; Raghavan, R. S.; Ranucci, G.; Razeto, A.; Re, A.; Romani, A.; Sabelnikov, A.; Saldanha, R.; Salvo, C.; Schönert, S.; Simgen, H.; Skorokhvatov, M.; Smirnov, O.; Sotnikov, A.; Sukhotin, S.; Suvorov, Y.; Tartaglia, R.; Testera, G.; Vignaud, D.; Vogelaar, R. B.; von Feilitzsch, F.; Winter, J.; Wojcik, M.; Wright, A.; Wurm, M.; Xu, J.; Zaimidoroga, O.; Zavatarelli, S.; Zuzel, G.

2012-02-01

We observed, for the first time, solar neutrinos in the 1.0-1.5 MeV energy range. We determined the rate of pep solar neutrino interactions in Borexino to be 3.1±0.6stat±0.3systcounts/(day·100ton). Assuming the pep neutrino flux predicted by the standard solar model, we obtained a constraint on the CNO solar neutrino interaction rate of Mikheyev-Smirnov-Wolfenstein large mixing angle solution to solar neutrino oscillations, these values correspond to solar neutrino fluxes of (1.6±0.3)×108cm-2s-1 and <7.7×108cm-2s-1 (95% C.L.), respectively, in agreement with both the high and low metallicity standard solar models. These results represent the first direct evidence of the pep neutrino signal and the strongest constraint of the CNO solar neutrino flux to date.

Optimizing the injection straight of PEP II asymmetric B Factory at SLAC

International Nuclear Information System (INIS)

Bulos, F.; Bloom, E.; Davies-White, W.; Donald, M.; Fairfield, K.; Fieguth, T.; Godfrey, G.; Holtzapple, R.; Hutton, A.; Loew, G.; Miller, R.; Sukiennicki, B.; Wen, H.; Ronan, M.

1992-04-01

The asymmetric energy PEP II B Factory proposed as an upgrade of PEP at the Stanford Linear Accelerator Center requires both a powerful low emittance source of e - e + and a very reliable and efficient injection system. The SLC linac fulfills the source requirement very well. We describe here the optimization of the optics of the injection straight to insure reliable and efficient injection

Optimizing the injection straight of PEP II asymmetric B factory at SLAC

International Nuclear Information System (INIS)

Bulos, F.; Bloom, E.; Davies-White, W.; Donald, M.; Fairfield, K.; Fieguth, T.; Godfrey, G.; Holtzapple, R.; Hutton, A.; Loew, G.; Miller, R.; Sukiennicki, B.; Wen, H.

1992-01-01

The asymmetric energy PEP II B Factory proposed as an upgrade of PEP at the Stanford Linear Accelerator Center requires both a powerful low emittance source of e - e + and a very reliable and efficient injection system. The SLC linac fulfills the source requirement very well. We describe here the optimization of the optics of the injection straight to insure reliable and efficient injection

Laboratory Tests on Post-Filtration Precipitation in the WTP Pretreatment Process

International Nuclear Information System (INIS)

Russell, Renee L.; Peterson, Reid A.; Rinehart, Donald E.; Crum, Jarrod V.

2009-01-01

Pacific Northwest National Laboratory (PNNL) has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to perform research and development activities to resolve technical issues identified for the Pretreatment Facility (PTF). The Pretreatment Engineering Platform (PEP) was designed, constructed, and operated as part of a plan to respond to issue M12, 'Undemonstrated Leaching Processes,' of the External Flowsheet Review Team (EFRT) issue response plan (Barnes et al. 2006). The PEP is a 1/4.5-scale test platform designed to simulate the WTP pretreatment caustic leaching, oxidative leaching, ultrafiltration solids concentration, and slurry washing processes. The PEP replicates the WTP leaching processes using prototypic equipment and control strategies. A simplified flow diagram of the PEP system is shown in Figure 1.1. Two operating scenarios are currently being evaluated for the ultrafiltration process (UFP) and leaching operations. The first scenario has caustic leaching performed in the UFP-2 ultrafiltration feed vessels (i.e., vessel UFP-VSL-T02A in the PEP; and vessels UFP-VSL-00002A and B in the WTP PTF). The second scenario has caustic leaching conducted in the UFP-1 ultrafiltration feed preparation vessels (i.e., vessels UFP-VSL-T01A and B in the PEP; vessels UFP-VSL-00001A and B in the WTP PTF).

Proline iminopeptidase PepI overexpressing Lactobacillus casei as an adjunct starter in Edam cheese

Science.gov (United States)

Navidghasemizad, Sahar; Takala, Timo M; Alatossava, Tapani; Saris, Per EJ

2013-01-01

In this study the growth of genetically modified Lactobacillus casei LAB6, overexpressing proline iminopeptidase PepI and its capacity to increase free proline was investigated during ripening of Edam cheese. The strain successfully survived 12 weeks of ripening period in cheese. The food-grade plasmid pLEB604, carrying the pepI gene, was stable, and PepI enzyme was active in LAB6 cells isolated at different stages of the ripening process. However, HPLC analyses indicated that Lb. casei LAB6 could not increase the amount of free proline in ripened cheese. PMID:23851577

Design and mechanism of action of a novel bacteria-selective antimicrobial peptide from the cell-penetrating peptide Pep-1

International Nuclear Information System (INIS)

Zhu, W.L.; Lan Hongliang; Park, Il-Seon; Kim, Jae Il; Jin, H.Z.; Hahm, Kyung-Soo; Shin, S.Y.

2006-01-01

Here, we report the successful design of a novel bacteria-selective antimicrobial peptide, Pep-1-K (KKTWWKTWWTKWSQPKKKRKV). Pep-1-K was designed by replacing Glu-2, Glu-6, and Glu-11 in the cell-penetrating peptide Pep-1 with Lys. Pep-1-K showed strong antibacterial activity against reference strains (MIC = 1-2 μM) of Gram-positive and Gram-negative bacteria as well as against clinical isolates (MIC = 1-8 μM) of methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa. In contrast, Pep-1-K did not cause hemolysis of human erythrocytes even at 200 μM. These results indicate that Pep-1-K may be a good candidate for antimicrobial drug development, especially as a topical agent against antibiotic-resistant microorganisms. Tryptophan fluorescence studies indicated that the lack of hemolytic activity of Pep-1-K correlated with its weak ability to penetrate zwitterionic phosphatidylcholine/cholesterol (10:1, w/w) vesicles, which mimic eukaryotic membranes. Furthermore, Pep-1-K caused little or no dye leakage from negatively charged phosphatidylethanolamine/phosphatidylglycerol (7:3, w/w) vesicles, which mimic bacterial membranes but had a potent ability to cause depolarization of the cytoplasmic membrane potential of intact S. aureus cells. These results suggested that Pep-1-K kills microorganisms by not the membrane-disrupting mode but the formation of small channels that permit transit of ions or protons but not molecules as large as calcein

Lower Secondary School Students' Attitudes Toward Computer-Supported Laboratory Exercises

Directory of Open Access Journals (Sweden)

Andreja Špernjak

2010-03-01

Full Text Available In Science teaching laboratory work is recognized as one of the cornerstones. In school science laboratory work computers can be used as computer supported laboratory (real and as virtual laboratory. In the first case “real” laboratories involve bench top experiments utilizing data acquisition systems while “virtual” laboratory entails interactive simulations and animations. Lower secondary school students in age between 11 and 15 performed three laboratory exercises (Activity of yeast, Gas exchange in breathing, Heart rate as classic, computer-supported and virtual laboratory. As a result of testing we know that all three methods are suitable even for younger students. When they were asked which method they liked the most, their first choice was computer-supported laboratory, followed by classic laboratory, and virtual laboratory at the end. Additionally recognized weak and strong sides of used methods are discussed.

BioPepDB: an integrated data platform for food-derived bioactive peptides.

Science.gov (United States)

Li, Qilin; Zhang, Chao; Chen, Hongjun; Xue, Jitong; Guo, Xiaolei; Liang, Ming; Chen, Ming

2018-03-12

Food-derived bioactive peptides play critical roles in regulating most biological processes and have considerable biological, medical and industrial importance. However, a large number of active peptides data, including sequence, function, source, commercial product information, references and other information are poorly integrated. BioPepDB is a searchable database of food-derived bioactive peptides and their related articles, including more than four thousand bioactive peptide entries. Moreover, BioPepDB provides modules of prediction and hydrolysis-simulation for discovering novel peptides. It can serve as a reference database to investigate the function of different bioactive peptides. BioPepDB is available at http://bis.zju.edu.cn/biopepdbr/ . The web page utilises Apache, PHP5 and MySQL to provide the user interface for accessing the database and predict novel peptides. The database itself is operated on a specialised server.

Status report of PEP-4

International Nuclear Information System (INIS)

Kamae, Tuneyoshi

1982-01-01

The present status of PEP-4 is reported. The cosmic ray test of a TPC without magnetic field was done in July, 1981, and tracks were observed on the first day of the test. The entire PEP-4 detector was tested with 3.9 kG magnetic field in November, 1981, and various trigger configurations were tested. The TPC performed well during the test, and a preliminary analysis gave an encouraging position resolution which is expected to be improved with the correction for various non-uniformity and by refining the fitting procedure. Furthermore, the detection efficiency of the muon chamber was about 99 %. In November and December, 1981, the cosmic ray test of the hexagonal calorimeter was carried out. The performance was just as expected. Noise did not pose any problem, and the peak of the Fourier coefficient gave a nice overall calibration of the calorimeter. There are some problems to be solved in future. With the TPC, the impurities in the gas, the electronic noise, the hooks and the magnetic coil should be improved. In addition, there are some problems with the inner drift chamber, the hexagonal calorimeter and the relativistic rise measured by the TPC. The very recent results with the first e + e - beam obtained in February, 1982, are briefly reported, although the events have not been fully analyzed. (Ito, K.)

Topology in quantum states. PEPS formalism and beyond

Energy Technology Data Exchange (ETDEWEB)

Aguado, M [Max-Planck-Institut fuer Quantenoptik. Hans-Kopfermann-Str. 1. D-85748 Garching (Germany); Cirac, J I [Max-Planck-Institut fuer Quantenoptik. Hans-Kopfermann-Str. 1. D-85748 Garching (Germany); Vidal, G [School of Physical Sciences. University of Queensland, Brisbane, QLD, 4072 (Australia)

2007-11-15

Topology has been proposed as a tool to protect quantum information encoding and processes. Work concerning the meaning of topology in quantum states as well as its characterisation in the projected entangled pair state (PEPS) formalism and related schemes is reviewed.

Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model

International Nuclear Information System (INIS)

Kwon, Soon Won; Sohn, Eun Jeong; Kim, Dae Won; Jeong, Hoon Jae; Kim, Mi Jin; Ahn, Eun Hee; Kim, Young Nam; Dutta, Suman; Kim, Duk-Soo; Park, Jinseu; Eum, Won Sik; Hwang, Hyun Sook; Choi, Soo Young

2011-01-01

Highlights: → Recombinant PEP-1 heme oxygenase-1 expression vector was constructed and overexpressed. → We investigated transduction efficiency of PEP-1-HO-1 protein in Raw 264.7 cells. → PEP-1-HO-1 was efficiently transduced into Raw 264.7 cells in a dose and time dependent manner. → PEP-1-HO-1 exerted anti-inflammatory activity in Raw 264.7 cells and in a mice edema model. → PEP-1-HO-1 could be used as a therapeutic drug against inflammatory diseases. -- Abstract: Heme oxygenase-1 (HO-1), which catalyzes the degradation of free heme to biliverdin, carbon monoxide (CO), and free iron (Fe 2+ ), is up-regulated by several cellular stress and cell injuries, including inflammation, ischemia and hypoxia. In this study, we examined whether fusion of HO-1 with PEP-1, a protein transduction domain that is able to deliver exogenous molecules to living cells or tissues, would facilitate HO-1 delivery to target cells and tissues, and thereby effectively exert a therapeutically useful response against inflammation. Western blot analysis demonstrated that PEP-1-HO-1 fusion proteins were transduced into Raw 264.7 cells in time- and dose-dependent manners, and were stably maintained in the cells for about 60 h. In addition, fluorescence analysis revealed that only PEP-1-HO-1 fusion proteins were significantly transduced into the cytoplasm of cells, while HO-1 proteins failed to be transduced. In lipopolysaccharide (LPS)-stimulated Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse edema model, transduced PEP-1-HO-1 fusion proteins effectively inhibited the overexpression of pro-inflammatory mediators and cytokines. Also, histological analysis demonstrated that PEP-1-HO-1 remarkably suppressed ear edema. The results suggest that the PEP-1-HO-1 fusion protein can be used as a therapeutic molecule against reactive oxygen species-related inflammatory diseases.

Detecting β-thalassaemia mutations from a single cell by PEP and RDB

Institute of Scientific and Technical Information of China (English)

YI Ping; LI Li; YAO Hong; ZHOU Yuan-guo; DENG Bing; CHEN Zhu-qin

2006-01-01

Objective:To evaluate the possibility of the technology involving PEP and RDB for detecting β-thalassaemia multipoint mutations from a single cell simultaneously. Methods: A set of allele specific oligonucleotide (ASO) probes used for detecting 8 familiar β-thalassaemia mutations (CD41-42, IVS- Ⅱ -654, CD17, TATA box nt-28, CD71-72, TATA box nt-29, CD26, IVS- Ⅰ -5) were immobilized on a strip of nylon membrane. The genome of a individual cell was amplified by primer extension preamplification (PEP) with the mixture of15-base random oligonucleotides. The aliquots from PEP were used to amplify the objective gene fractions of β-thalassaemia gene by nested or semi-nested PCR. The membrane was hybridized with the final amplified products and then treated with Streptavidin-HRP and color development.Results :Totally 30 lymphocytes were picked up from blood samples of 1 healthy female and 4 patients with known β-thalassaemia mutations respectively. Each single lymphocyte was lysed in the proteinase K buffer. The amplification efficacy was 94.0% and alle drop-out(ADO) rate was 8.0%. Revert dot blot (RDB) was applied to the final amplified products from the 5 participants. The results of diagnosis were the same to the expected, and their genotypes were N/N, CD17 (A→T)/N, IVS- Ⅱ -654(C→T)/CD17(A → T), CD41-42 (-CTTT)/N and TATA box nt-28 (A→G)/N, respectively. Conclusion: The technology involving PEP and RDB could detectmultiple β-thalassaemia mutations from a single cell simultaneously,and the research provides experimental evidences for the feasibility of applying PEP and DNA array technology to screening multiple genetic mutations from a single cell, and will be applied to preimplantation genetic diagnosis and non-invasive prenatal diagnosis for β-thalassaemia.

Review of e+e- physics at PEP - 1982

International Nuclear Information System (INIS)

Hollebeek, R.

1982-10-01

Results obtained at PEP by the MARK II, MAC and DELCO collaborations in the past year are reviewed. They include QED tests, measurements of tau lepton properties, charm and bottom fragmentation functions, scaling violations, energy correlations and the total hadronic cross section

Some schemes for on-line correction of the closed orbit, dispersion and beta functions in PEP

International Nuclear Information System (INIS)

Donald, M.H.R.; Blocker, C.; Chao, A.W.; Hollebeek, R.J.; Lee, M.J.; Linstadt, J.E.; Siegrist, J.L.; Spencer, N.

1981-02-01

PEP has been operated successfully under computer control. It is necessary for colliding beam operation that the errors in closed orbits, dispersion and beta functions be corrected. The schemes in the PEP control program for on-line correction of these errors are described in this paper. The orbit control tasks in the PEP control system perform the functions of data gathering, data presentation (color display, printing), calculation and setting of corrector magnets. The tasks are generally small and modular, taking information from the database, processing it, then returning the results to the database. The PEP operator communicates with the tasks through touch panels monitored by the Director program. The display task, which displays orbit and corrector information on a TV color display, provides the main information required by the operator

Design and performance of PEP dc-power systems

International Nuclear Information System (INIS)

Jackson, T.

1981-03-01

The PEP Magnet Power Supply System represents a significant departure from previous technology with the goal of improved performance at lower cost. In nineteen of the magnet families around the ring, Chopper power supplies are used. The many choppers are powered from two 2 MW dc supplies, and control the average power to the various magnet loads by pulse-width modulation at a 2 kilohertz repetition rate. Each chopper utilizes SCR's for switching, and stores sufficient capacitive energy for turn-off on command. Most of the energy is recirculated, resulting in high-efficiency. The two kilohertz chopping rate allows a one kilohertz unity-gain bandwidth in the current-regulator loop, and this wide bandwidth, coupled with low drift components in the error-detection system, provides a high-performance system. The PEP system has also shown that the chopper system is economical compared to standard multi-pulse controlled-rectifier

Vacuum system design for the PEP-II B Factory High-Energy Ring

International Nuclear Information System (INIS)

Perkins, C.; Bostic, D.; Daly, E.

1994-06-01

The design of the vacuum system for the PEP-II B Factory High-Energy Ring is reviewed. The thermal design and vacuum requirements are particularly challenging in PEP-II due to high stored beam currents up to 3.0 amps in 1658 bunches. The vacuum chambers for the HER arcs are fabricated by electron beam welding extruded copper sections up to 6 m long. Design of these chambers and the vacuum PumPing configuration is described with results from vacuum and thermal analyses

[Knowledge management system for laboratory work and clinical decision support].

Science.gov (United States)

Inada, Masanori; Sato, Mayumi; Yoneyama, Akiko

2011-05-01

This paper discusses a knowledge management system for clinical laboratories. In the clinical laboratory of Toranomon Hospital, we receive about 20 questions relevant to laboratory tests per day from medical doctors or co-medical staff. These questions mostly involve the essence to appropriately accomplish laboratory tests. We have to answer them carefully and suitably because an incorrect answer may cause a medical accident. Up to now, no method has been in place to achieve a rapid response and standardized answers. For this reason, the laboratory staff have responded to various questions based on their individual knowledge. We began to develop a knowledge management system to promote the knowledge of staff working for the laboratory. This system is a type of knowledge base for assisting the work, such as inquiry management, laboratory consultation, process management, and clinical support. It consists of several functions: guiding laboratory test information, managing inquiries from medical staff, reporting results of patient consultation, distributing laboratory staffs notes, and recording guidelines for laboratory medicine. The laboratory test information guide has 2,000 records of medical test information registered in the database with flexible retrieval. The inquiry management tool provides a methos to record all questions, answer easily, and retrieve cases. It helps staff to respond appropriately in a short period of time. The consulting report system treats patients' claims regarding medical tests. The laboratory staffs notes enter a file management system so they can be accessed to aid in clinical support. Knowledge sharing using this function can achieve the transition from individual to organizational learning. Storing guidelines for laboratory medicine will support EBM. Finally, it is expected that this system will support intellectual activity concerning laboratory work and contribute to the practice of knowledge management for clinical work support.

Upgrades to PEP-II Tune Measurements

Energy Technology Data Exchange (ETDEWEB)

Fisher, Alan S.

2002-07-30

The tune monitors for the two-ring PEP-II collider convert signals from one set of four BPM-type pickup buttons per ring into horizontal and vertical differences, which are then downconverted from 952 MHz (twice the RF) to baseband. Two-channel 10-MHz FFT spectrum analyzers show spectra in X-window displays in the Control Room, to assist PEP operators. When operating with the original system near the beam-beam limit, collisions broadened and flattened the tune peaks, often bringing them near the noise floor. We recently installed new downconverters that increase the signal-to-noise ratio by about 5 dB. In addition, we went from one to two sets of pickups per ring, near focusing and defocusing quadrupoles, so that signals for both planes originate at locations with large amplitudes. We also have just installed a tune tracker, based on a digital lock-in amplifier (one per tune plane) that is controlled by an EPICS software feedback loop. The tracker monitors the phase of the beam's response to a sinusoidal excitation, and adjusts the drive frequency to track the middle of the 1 go-degree phase transition across the tune resonance. We plan next to test an outer loop controlling the tune quadrupoles based on this tune measurement.

Upgrades to PEP-II Tune Measurements

Energy Technology Data Exchange (ETDEWEB)

Fisher, Alan S.

2002-07-30

The tune monitors for the two-ring PEP-II collider convert signals from one set of four BPM-type pickup buttons per ring into horizontal and vertical differences, which are then downconverted from 952 MHz (twice the RF) to baseband. Two-channel l0-MHz FFT spectrum analyzers show spectra in X-window displays in the Control Room, to assist PEP operators. When operating with the original system near the beam-beam limit, collisions broadened and flattened the tune peaks, often bringing them near the noise floor. We recently installed new downconverters that increase the signal-to-noise ratio by about 5 dB. In addition, we went from one to two sets of pickups per ring, near focusing and defocusing quadrupoles, so that signals for both planes originate at locations with large amplitudes. We also have just installed a tune tracker, based on a digital lock-in amplifier (one per tune plane) that is controlled by an EPICS software feedback loop. The tracker monitors the phase of the beam's response to a sinusoidal excitation, and adjusts the drive frequency to track the middle of the 180-degree phase transition across the tune resonance. We plan next to test an outer loop controlling the tune quadrupoles based on this tune measurement.

Upgrades to PEP-II tune measurements

International Nuclear Information System (INIS)

Fisher, Alan S.; Petree, Mark; Wienands, Uli; Allison, Stephanie; Laznovsky, Michael; Seeman, Michael; Robin, Jolene

2002-01-01

The tune monitors for the two-ring PEP-II collider convert signals from one set of four BPM-type pickup buttons per ring into horizontal and vertical differences, which are then downconverted from 952 MHz (twice the RF) to baseband. Two-channel 10-MHz FFT spectrum analyzers show spectra in X-window displays in the Control Room, to assist PEP operators. When operating with the original system near the beam-beam limit, collisions broadened and flattened the tune peaks, often bringing them near the noise floor. We recently installed new downconverters that increase the signal-to-noise ratio by about 5 dB. In addition, we went from one to two sets of pickups per ring, near focusing and defocusing quadrupoles, so that signals for both planes originate at locations with large amplitudes. We also have just installed a tune tracker, based on a digital lock-in amplifier (one per tune plane) that is controlled by an EPICS software feedback loop. The tracker monitors the phase of the beam's response to a sinusoidal excitation, and adjusts the drive frequency to track the middle of the 180-degree phase transition across the tune resonance. We plan next to test an outer loop controlling the tune quadrupoles based on this tune measurement

Theory of the Protein Equilibrium Population Snapshot by H/D Exchange Electrospray Ionization Mass Spectrometry (PEPS-HDX-ESI-MS) Method used to obtain Protein Folding Energies/Rates and Selected Supporting Experimental Evidence.

Science.gov (United States)

Liyanage, Rohana; Devarapalli, Nagarjuna; Pyland, Derek B; Puckett, Latisha M; Phan, N H; Starch, Joel A; Okimoto, Mark R; Gidden, Jennifer; Stites, Wesley E; Lay, Jackson O

2012-12-15

Protein equilibrium snapshot by hydrogen/deuterium exchange electrospray ionization mass spectrometry (PEPS-HDX-ESI-MS or PEPS) is a method recently introduced for estimating protein folding energies and rates. Herein we describe the basis for this method using both theory and new experiments. Benchmark experiments were conducted using ubiquitin because of the availability of reference data for folding and unfolding rates from NMR studies. A second set of experiments was also conducted to illustrate the surprising resilience of the PEPS to changes in HDX time, using staphylococcal nuclease and time frames ranging from a few seconds to several minutes. Theory suggests that PEPS experiments should be conducted at relatively high denaturant concentrations, where the protein folding/unfolding rates are slow with respect to HDX and the life times of both the closed and open states are long enough to be sampled experimentally. Upon deliberate denaturation, changes in folding/unfolding are correlated with associated changes in the ESI-MS signal upon fast HDX. When experiments are done quickly, typically within a few seconds, ESI-MS signals, corresponding to the equilibrium population of the native (closed) and denatured (open) states can both be detected. The interior of folded proteins remains largely un-exchanged. Amongst MS methods, the simultaneous detection of both states in the spectrum is unique to PEPS and provides a "snapshot" of these populations. The associated ion intensities are used to estimate the protein folding equilibrium constant (or the free energy change, ΔG). Linear extrapolation method (LEM) plots of derived ΔG values for each denaturant concentration can then be used to calculate ΔG in the absence of denaturant, ΔG(H(2)O). In accordance with the requirement for detection of signals for both the folded and unfolded states, this theoretical framework predicts that PEPS experiments work best at the middle of the denaturation curve where natured

Beam-beam depolarization in SPEAR and PEP

International Nuclear Information System (INIS)

Montague, B.W.

1977-01-01

In this note some approximate estimates are made of depolarization due to beam-beam forces in SPEAR and PEP, using the results of a calculation by Kondratenko. The model assumes head-on collisions between bunches of Gaussian distribution in the transverse directions; the force on the weak-beam particle is taken to be a δ-function at the interaction point. 1 ref

CNO and pep neutrino spectroscopy in Borexino: Measurement of the deep underground production of cosmogenic 11C in organic liquid scintillator

OpenAIRE

Borexino Collaboration

2006-01-01

Borexino is an experiment for low energy neutrino spectroscopy at the Gran Sasso underground laboratories. It is designed to measure the mono-energetic $^7$Be solar neutrino flux in real time, via neutrino-electron elastic scattering in ultra-pure organic liquid scintillator. Borexino has the potential to also detect neutrinos from the \\emph{pep} fusion process and the CNO cycle. For this measurement to be possible, radioactive contamination in the detector must be kept extremely low. Once su...

Laboratory-supported influenza surveillance in Victorian sentinel general practices.

Science.gov (United States)

Kelly, H; Murphy, A; Leong, W; Leydon, J; Tresise, P; Gerrard, M; Chibo, D; Birch, C; Andrews, R; Catton, M

2000-12-01

Laboratory-supported influenza surveillance is important as part of pandemic preparedness, for identifying and isolating candidate vaccine strains, for supporting trials of anti-influenza drugs and for refining the influenza surveillance case definition in practice. This study describes the implementation of laboratory-supported influenza surveillance in Victorian sentinel general practices and provides an estimate of the proportion of patients with an influenza-like illness proven to have influenza. During 1998 and 1999, 25 sentinel general practices contributed clinical surveillance data and 16 metropolitan practices participated in laboratory surveillance. Serological, virus-antigen detection, virus culture and multiplex polymerase chain reaction procedures were used to establish the diagnosis of influenza. Two laboratories at major teaching hospitals in Melbourne provided additional data on influenza virus identification. General practice sentinel surveillance and laboratory identification of influenza provided similar data on the pattern of influenza in the community between May and September. The clinical suspicion of influenza was confirmed in 49 to 54 per cent of cases seen in general practice.

Crystal Ball at PEP

International Nuclear Information System (INIS)

Bartel, W.; Bulos, F.; Luke, D.; Peck, C.; Strauch, K.

1975-01-01

The modifications to the SPEAR version of the Crystal Ball required by the higher energies at PEP are discussed. Since the hadron multiplicity is expected to rise as log s, their average energy must rise. On the other hand, if the hadrons are produced in jets, the low energy part of their spectrum is not heavily depleted. This implies that modifications for high energy particles should not deteriorate low energy performance. An external iron calorimeter for measuring the high energy hadrons, charged and neutral, is considered. To improve the angular resolution on γ's, an active internal converter has been studied, estimates have been made of its expected performance, and difficulties requiring further study have been outlined

τ physics using the TPC/2γ facility at HiLum PEP

International Nuclear Information System (INIS)

Bloom, E.D.

1991-01-01

Plans for a TPC/2γ facility program using HiLum PEP for τ physics are presented. The detector and its performance are described in some detail. The possibilities for a τ physics program with an integrated luminosity of 1 fb -1 are discussed. The physics issues are reviewed, particularly the τ'1-prong' problem, and preliminary results on τ → ν τ K +- X + X - + ≥ 0 Neutrals are shown. The performance of HiLum PEP is also presented, and expectations for the future are discussed. (R.P.) 15 refs., 14 figs., 3 tabs

Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry

NARCIS (Netherlands)

Pavlova, Ivelina; Milanova, Aneliya; Danova, Svetla; Fink-Gremmels, Johanna

2016-01-01

Expression of peptide transporter 1 (PepT1) and liver-expressed antimicrobial peptide 2 (LEAP-2) in chickens can be influenced by food deprivation, pathological conditions and drug administration. Effect of three putative probiotic Lactobacillus strains and enrofloxacin on the expression of PepT1

PEP activity and expression of photosynthesis genes required for embryo and seed development in Arabidopsis

Directory of Open Access Journals (Sweden)

Dmitry eKremnev

2014-08-01

Full Text Available Chloroplast biogenesis and function is essential for proper plant embryo and seed development but the molecular mechanisms underlying the role of plastids during embryogenesis are poorly understood. Expression of plastid encoded genes is dependent on two different transcription machineries; a plastid-encoded bacterial-type RNA polymerase (PEP and a nuclear-encoded phage-type RNA polymerase (NEP, which recognize distinct types of promoters. However, the division of labor between PEP and NEP during plastid development and in mature chloroplasts is unclear. We show here that PRIN2 and CSP41b, two proteins identified in plastid nucleoid preparations, are essential for proper plant embryo development. Using Co-IP assays and native PAGE we have shown a direct physical interaction between PRIN2 and CSP41b. Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. The respective csp41b-2prin2.2 homo/heterozygote double mutants produced abnormal white colored ovules and shrunken seeds. Thus, the csp41b-2prin2.2 double mutant is embryo lethal. In silico analysis of available array data showed that a large number of genes traditionally classified as PEP dependent genes are transcribed during early embryo development from the pre-globular stage to the mature-green-stage. Taken together, our results suggest that PEP activity and consequently the switch from NEP to PEP activity, is essential during embryo development and that the PRIN2-CSP41b DNA binding protein complex possibly is important for full PEP activity during this process.

Transduced human copper chaperone for Cu,Zn-SOD (PEP-1-CCS) protects against neuronal cell death.

Science.gov (United States)

Choi, Soo Hyun; Kim, Dae Won; Kim, So Young; An, Jae Jin; Lee, Sun Hwa; Choi, Hee Soon; Sohn, Eun Jung; Hwang, Seok-Il; Won, Moo Ho; Kang, Tae-Cheon; Kwon, Hyung Joo; Kang, Jung Hoon; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

2005-12-31

Reactive oxygen species (ROS) contribute to the development of various human diseases. Cu,Zn-superoxide dismutase (SOD) is one of the major means by which cells counteract the deleterious effects of ROS. SOD activity is dependent upon bound copper ions supplied by its partner metallochaperone protein, copper chaperone for SOD (CCS). In the present study, we investigated the protective effects of PEP-1-CCS against neuronal cell death and ischemic insults. When PEP-1-CCS was added to the culture medium of neuronal cells, it rapidly entered the cells and protected them against paraquat-induced cell death. Moreover, transduced PEP-1-CCS markedly increased endogenous SOD activity in the cells. Immunohistochemical analysis revealed that it prevented neuronal cell death in the hippocampus in response to transient forebrain ischemia. These results suggest that CCS is essential to activate SOD, and that transduction of PEP-1-CCS provides a potential strategy for therapeutic delivery in various human diseases including stroke related to SOD or ROS.

Pep1, a secreted effector protein of Ustilago maydis, is required for successful invasion of plant cells.

Directory of Open Access Journals (Sweden)

Gunther Doehlemann

2009-02-01

Full Text Available The basidiomycete Ustilago maydis causes smut disease in maize. Colonization of the host plant is initiated by direct penetration of cuticle and cell wall of maize epidermis cells. The invading hyphae are surrounded by the plant plasma membrane and proliferate within the plant tissue. We identified a novel secreted protein, termed Pep1, that is essential for penetration. Disruption mutants of pep1 are not affected in saprophytic growth and develop normal infection structures. However, Deltapep1 mutants arrest during penetration of the epidermal cell and elicit a strong plant defense response. Using Affymetrix maize arrays, we identified 116 plant genes which are differentially regulated in Deltapep1 compared to wild type infections. Most of these genes are related to plant defense. By in vivo immunolocalization, live-cell imaging and plasmolysis approaches, we detected Pep1 in the apoplastic space as well as its accumulation at sites of cell-to-cell passages. Site-directed mutagenesis identified two of the four cysteine residues in Pep1 as essential for function, suggesting that the formation of disulfide bridges is crucial for proper protein folding. The barley covered smut fungus Ustilago hordei contains an ortholog of pep1 which is needed for penetration of barley and which is able to complement the U. maydis Deltapep1 mutant. Based on these results, we conclude that Pep1 has a conserved function essential for establishing compatibility that is not restricted to the U. maydis / maize interaction.

Development of a high-power RF cavity for the PEP-II B factory

International Nuclear Information System (INIS)

Rimmer, R.A.; Allen, M.A.; Saba, J.; Schwarz, H.

1995-03-01

The authors describe the development and fabrication of the first high-power RF cavity for PEP-II. Design choices and fabrication technologies for the first cavity and subsequent production cavities are described. Conditioning and high-power testing of the first and subsequent cavities are discussed, as well as integration of the cavity into modular RF systems for both high-energy and low-energy rings. Plans for installation of the cavity raft assemblies in the RF sections of the PEP tunnel are also considered

On setting magnets in the PEP beam-transport line

International Nuclear Information System (INIS)

Peterson, J.M.

1979-01-01

This paper discusses magnets in the PEP beam-transport line. Topics discussed are: conditioning, direction of excitation, rate of excitation; determination of the excitation current for the principal bend magnets; steering mechanisms; bump magnets; and determination of excitation currents of the quadrupole magnets

Awareness and practice of safety precautions among healthcare workers in the laboratories of two public health facilities in Nigeria.

Science.gov (United States)

Fadeyi, A; Fowotade, A; Abiodun, M O; Jimoh, A K; Nwabuisi, C; Desalu, O O

2011-06-01

To determine the level of awareness and practice of SP among laboratory workers at two tertiary public health facilities in Nigeria. A semi-structured, self-administered questionnaire was used to assess the awareness, attitude and adherence to SP among laboratory workers. Information on the availability of safety equipment was also sought. The laboratory safety practice of respondents was assessed based on self-reported observance of basic principles of universal precautions in clinical settings. Study participants were 130, mean age: 28.2 years (SD±6.6), number of years in hospital employment: 3.7 years (SD±2.4) and the male to female ratio was 1.8:1. Many (41.5%) were unaware and 25.4% do not observe SP. Participants attest to availability of various safety devices and equipment including hand gloves (86.2%), disinfectants (84.6%), HBV immunisation (46.2%) and post exposure prophylaxis (PEP) for HIV and HBV (79.6%). Attitude to safety is unsatisfactory as 60.0% eat and drink in the laboratory, 50.8% recap needles and 56.9% use sharps box. Even though 83.1% are willing to take PEP, only 1.5% will present self following laboratory injury. This study shows the deficit in the awareness of SP among laboratory personnel and demonstrates that attitude and practice of safety rules are unsatisfactory. Training and re-training on SP is therefore desired. Counselling to induce a positive attitudinal change on HBV immunisation and PEP is similarly necessary.

The Optical Design of the PEP-II Injection Beamlines

CERN Document Server

Fieguth, T

1996-01-01

The optical design of the PEP-II electron and positron Injection Beamlines is described. Use of the existing high power, low emittance beams available from the SLC damping rings require that pulsed extraction of 9.0 GeV electrons and 3.1 GeV positrons for injection into the PEP-II rings occur in the early sectors of the accelerator. More than 5 kilometers of new beam transport lines have been designed and are being constructed to bring these beams to their respective rings. The optical design maximizes the tolerance to errors especially to those contributing to beam size and position jitter. Secondly, the design minimizes costs by utilizing existing components or component designs and minimizing the number required. Here we discuss important attributes including choice of lattice, specification of error tolerances, including errors in construction, alignment, field errors, power supply stability, and orbit correction.

Transverse feedback systems for the PEP-II B-factory

International Nuclear Information System (INIS)

Corlett, J.N.; Barry, W.; Byrd, J.M.; Lambertson, G.; Johnson, J.; Fahmie, M.

1995-10-01

Growth rates of coherent beam oscillations are faster than the natural damping mechanisms for the parameters of the PEP-II B-factory storage rings at nominal currents, even with damping of cavity higher order modes (HOM's). With 165 8 bunches separated by 4.2 ns, and a large current of up to 3A (2.14 A nominal in the low energy ring), many coupled-bunch modes are excited by the resistive wall impedance and cavity higher order mode impedance. Fastest growth times of transverse rigid-bunch modes of approximately 300 ps are expected, two orders of magnitude faster than the radiation damping time. We will provide broadband, bunch-by-bunch feedback to suppress this coherent motion of the beam. Experience gained with a prototype system, installed and successfully operating at the LBNL Advanced Light Source (ALS), has been used extensively in developing the design of the PEP-II systems

The Optical Design of the PEP-II Injection Beamlines

Energy Technology Data Exchange (ETDEWEB)

Fieguth, Ted

2003-05-23

The optical design of the PEP-II electron and positron Injection Beamlines is described. Use of the existing high power, low emittance beams available from the SLC damping rings require that pulsed extraction of 9.0 GeV electrons and 3.1 GeV positrons for injection into the PEP-II rings occur in the early sectors of the accelerator. More than 5 kilometers of new beam transport lines have been designed and are being constructed to bring these beams to their respective rings. The optical design maximizes the tolerance to errors especially to those contributing to beam size and position jitter. Secondly, the design minimizes costs by utilizing existing components or component designs and minimizing the number required. Here we discuss important attributes including choice of lattice, specification of error tolerances, including errors in construction, alignment, field errors, power supply stability, and orbit correction.

The PEP-II Project: Low-Energy Ring Design and Project Status

International Nuclear Information System (INIS)

Zisman, Michael S.

2006-01-01

We describe the present status of the PEP-II project. The project comprises four major systems: Injector, High-Energy Ring (HER), Low-Energy Ring (LER), and Interaction Region (IR). We focus in detail on the design of the LER, as its parameters and requirements are most closely related to those required for the Beijing Tau-Charm Factory rings. The PEP-II LER is a high-current, 3.1-GeV positron ring mounted above the 9-GeV HER. The LER uses a wiggler located in one of its six straight sections to provide emittance control and additional damping. We describe the rather complicated IR, which must transport the LER beam into the plane of the HER, focus it to a common beam size, and separate the beams after the head-on collisions. Both permanent magnet and conventional electromagnets are used in this area. The LER lattice has now adopted a simplified non-interleaved sextupole correction scheme that has reduced the required number of sextupoles substantially. We describe the LER vacuum system, one of the most challenging subsystems in PEP-II. It employs several technologies. In the arcs, aluminum extrusions and titanium sublimation pumps are employed; the straight sections use stainless steel chambers with lumped ion pumps. In the wiggler area, an extended copper photon dump with nonevaporable getter (NEG) pumps is employed to handle the very large synchrotron radiation power. The design of the room-temperature RF system, the bunch-by-bunch longitudinal and transverse feedback systems, and some of the special diagnostics will be described briefly. The PEP-II project remains on schedule to begin commissioning of the HER in April 1997, followed by the LER a year later

Progress on PEP-II magnet power conversion system

International Nuclear Information System (INIS)

Bellomo, P.; Genova, L.; Jackson, T.; Shimer, D.

1996-01-01

The various power systems for supplying the PEP-II DC magnets rely exclusively on switchmode conversion, utilizing a variety of means depending on the requirements. All of the larger power supplies, ranging from 10 to 200 kW, are powered from DC sources utilizing rectified 480 V AC. Choppers can be used for the series connected strings, but for smaller groups and individual magnets, inverters driving high-frequency transformers with rectifiers comprise the best approach. All of the various systems use a ''building block'' approach of multiple standard-size units connected in series or parallel to most cost-effectively deal with a great range of voltage and current requirements. Utilization of existing infrastructure from PEP-I has been a cost-effective determinant. Equipment is being purchased either off-the-shelf, through performance specification, or by hardware purchase based on design-through-prototype. The corrector magnet power system, utilizing inexpensive, off-the-shelf, four-quadrant switching motor-controllers, has already proven very reliable: 120 of the total of 900 units have been running on the injection system for four months with no failures

Progress on PEP-II magnet power conversion system

Energy Technology Data Exchange (ETDEWEB)

Bellomo, P.; Genova, L. [Stanford Linear Accelerator Center, Menlo Park, CA (United States); Jackson, T. [Lawrence Berkeley National Lab., CA (United States); Shimer, D. [Lawrence Livermore National Lab., CA (United States)

1996-06-04

The various power systems for supplying the PEP-II DC magnets rely exclusively on switchmode conversion, utilizing a variety of means depending on the requirements. All of the larger power supplies, ranging from 10 to 200 kW, are powered from DC sources utilizing rectified 480 V AC. Choppers can be used for the series connected strings, but for smaller groups and individual magnets, inverters driving high-frequency transformers with rectifiers comprise the best approach. All of the various systems use a ``building block`` approach of multiple standard-size units connected in series or parallel to most cost-effectively deal with a great range of voltage and current requirements. Utilization of existing infrastructure from PEP-I has been a cost-effective determinant. Equipment is being purchased either off-the-shelf, through performance specification, or by hardware purchase based on design-through-prototype. The corrector magnet power system, utilizing inexpensive, off-the-shelf, four-quadrant switching motor-controllers, has already proven very reliable: 120 of the total of 900 units have been running on the injection system for four months with no failures.

Psycho-education with problem solving (PEPS therapy for adults with personality disorder: A pragmatic multi-site community-based randomised clinical trial

Directory of Open Access Journals (Sweden)

Duggan Conor

2011-08-01

Full Text Available Abstract Background Impairment in social functioning is a key component of personality disorder. Therefore psycho-education and problem solving (PEPS therapy may benefit people with this disorder. Psycho-education aims to educate, build rapport, and motivate people for problem solving therapy. Problem solving therapy aims to help clients solve interpersonal problems positively and rationally, thereby improving social functioning and reducing distress. PEPS therapy has been evaluated with community adults with personality disorder in an exploratory trial. At the end of treatment, compared to a wait-list control group, those treated with PEPS therapy showed better social functioning, as measured by the Social Functioning Questionnaire (SFQ. A definitive evaluation is now being conducted to determine whether PEPS therapy is a clinically and cost-effective treatment for people with personality disorder Methods This is a pragmatic, two-arm, multi-centre, parallel, randomised controlled clinical trial. The target population is community-dwelling adults with one or more personality disorder, as identified by the International Personality Disorder Examination (IPDE. Inclusion criteria are: Living in the community (including residential or supported care settings; presence of one or more personality disorder; aged 18 or over; proficiency in spoken English; capacity to provide informed consent. Exclusion criteria are: Primary diagnosis of a functional psychosis; insufficient degree of literacy, comprehension or attention to be able to engage in trial therapy and assessments; currently engaged in a specific programme of psychological treatment for personality disorder or likely to start such treatment during the trial period; currently enrolled in any other trial. Suitable participants are randomly allocated to PEPS therapy plus treatment as usual (TAU or TAU only. We aim to recruit 340 men and women. The primary outcome is social functioning as measured

The HtrA-like serine protease PepD interacts with and modulates the Mycobacterium tuberculosis 35-kDa antigen outer envelope protein.

Directory of Open Access Journals (Sweden)

Mark J White

2011-03-01

Full Text Available Mycobacterium tuberculosis remains a significant global health concern largely due to its ability to persist for extended periods within the granuloma of the host. While residing within the granuloma, the tubercle bacilli are likely to be exposed to stress that can result in formation of aberrant proteins with altered structures. Bacteria encode stress responsive determinants such as proteases and chaperones to deal with misfolded or unfolded proteins. pepD encodes an HtrA-like serine protease and is thought to process proteins altered following exposure of M. tuberculosis to extra-cytoplasmic stress. PepD functions both as a protease and chaperone in vitro, and is required for aspects of M. tuberculosis virulence in vivo. pepD is directly regulated by the stress-responsive two-component signal transduction system MprAB and indirectly by extracytoplasmic function (ECF sigma factor SigE. Loss of PepD also impacts expression of other stress-responsive determinants in M. tuberculosis. To further understand the role of PepD in stress adaptation by M. tuberculosis, a proteomics approach was taken to identify binding proteins and possible substrates of this protein. Using subcellular fractionation, the cellular localization of wild-type and PepD variants was determined. Purified fractions as well as whole cell lysates from Mycobacterium smegmatis or M. tuberculosis strains expressing a catalytically compromised PepD variant were immunoprecipitated for PepD and subjected to LC-MS/MS analyses. Using this strategy, the 35-kDa antigen encoding a homolog of the PspA phage shock protein was identified as a predominant binding partner and substrate of PepD. We postulate that proteolytic cleavage of the 35-kDa antigen by PepD helps maintain cell wall homeostasis in Mycobacterium and regulates specific stress response pathways during periods of extracytoplasmic stress.

AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants.

Science.gov (United States)

Nakaminami, Kentaro; Okamoto, Masanori; Higuchi-Takeuchi, Mieko; Yoshizumi, Takeshi; Yamaguchi, Yube; Fukao, Yoichiro; Shimizu, Minami; Ohashi, Chihiro; Tanaka, Maho; Matsui, Minami; Shinozaki, Kazuo; Seki, Motoaki; Hanada, Kousuke

2018-05-29

Peptides encoded by small coding genes play an important role in plant development, acting in a similar manner as phytohormones. Few hormone-like peptides, however, have been shown to play a role in abiotic stress tolerance. In the current study, 17 Arabidopsis genes coding for small peptides were found to be up-regulated in response to salinity stress. To identify peptides leading salinity stress tolerance, we generated transgenic Arabidopsis plants overexpressing these small coding genes and assessed survivability and root growth under salinity stress conditions. Results indicated that 4 of the 17 overexpressed genes increased salinity stress tolerance. Further studies focused on AtPROPEP3 , which was the most highly up-regulated gene under salinity stress. Treatment of plants with synthetic peptides encoded by AtPROPEP3 revealed that a C-terminal peptide fragment (AtPep3) inhibited the salt-induced bleaching of chlorophyll in seedlings. Conversely, knockdown AtPROPEP3 transgenic plants exhibited a hypersensitive phenotype under salinity stress, which was complemented by the AtPep3 peptide. This functional AtPep3 peptide region overlaps with an AtPep3 elicitor peptide that is related to the immune response of plants. Functional analyses with a receptor mutant of AtPep3 revealed that AtPep3 was recognized by the PEPR1 receptor and that it functions to increase salinity stress tolerance in plants. Collectively, these data indicate that AtPep3 plays a significant role in both salinity stress tolerance and immune response in Arabidopsis .

Structure of PEP-PEO block copolymer micelles: Exploiting the complementarity of small-angle X-ray scattering and static light scattering

DEFF Research Database (Denmark)

Jensen, Grethe Vestergaard; Shi, Qing; Hernansanz, María J.

2011-01-01

)-b-poly(ethylene oxide) (PEP-PEO) in a 70% ethanol solution are investigated. The polymers have identical PEP blocks of 5.0 kDa and varying PEO blocks of 2.8-49 kDa. The SLS contrasts of PEP and PEO are similar, providing a homogeneous contrast, making SLS ideal for determining the overall micelle morphology. The SAXS...... contrasts of the two components are very different, allowing for resolution of the internal micelle structure. A core-shell model with a PEP core and PEO corona is fitted simultaneously to the SAXS and SLS data using the different contrasts of the two blocks for each technique. With increasing PEO molecular...

Locality at the boundary implies gap in the bulk for 2D PEPS

DEFF Research Database (Denmark)

Kastoryano, Michael J.; Lucia, Angelo; Perez-Garcia, David

2018-01-01

Proving that the parent Hamiltonian of a Projected Entangled Pair State (PEPS) is gapped remains an important open problem. We take a step forward in solving this problem by showing that if the boundary state of any rectangular subregion is a quasi-local Gibbs state of the virtual indices, then t...... boundary theories and dynamical properties in an interacting many body system. We show that the proof can be extended to MPO-injective PEPS, and speculate that the assumption on the locality of the boundary Hamiltonian follows from exponential decay of correlations in the bulk....

Design features and operational characteristics of the PEP beam-transport and injection system

International Nuclear Information System (INIS)

Peterson, J.M.; Brown, K.L.; Truher, J.B.

1981-03-01

The PEP beam-transport system was designed to transmit 4-to-15 GeV electron and positron beams from the SLAC linac within a +- 0.8% momentum band, to have flexible tuning of the betatron and off-momentum functions for matching into the PEP storage ring, and to have convenient operating characteristics. The transport lines were brought into operation quickly and have operated well. Electron injection has been consistent and efficient and relatively easy to accomplish. Positron injection also has been satisfactory but is variable and more sensitive to ring conditions

FTIR Laboratory in Support of the PV Program

International Nuclear Information System (INIS)

Keyes, B. M.; Gedvilas, L. M.; Bhattacharya, R.; Xu, Y.; Li, X.; Wang, Q.

2005-01-01

The Fourier Transform Infrared Spectroscopy (FTIR) Laboratory supports the Solar Energy Technologies Program through the measurement and characterization of solar energy-related materials and devices. The FTIR technique is a fast, accurate, and reliable method for studying molecular structure and composition. This ability to identify atomic species and their bonding environment is a powerful combination that finds use in many research and development efforts. A brief overview of the technical approach used is contained in Section 2 of this report. Because of its versatility and accessibility, the FTIR Laboratory is a valuable contributor to the Solar Energy Technologies Program. The laboratory provides support for, and collaborates with, several in-house programs as well as our industry and university partners. By the end of FY 2004, the FTIR Laboratory performed over 1100 measurements on PV-related materials. These contributions resulted in conference and workshop presentations and several peer-reviewed publications. A brief summary of a few of these efforts is contained in Section 3 of this report

PEP-II IR-2 Alignment

International Nuclear Information System (INIS)

Seryi, A

2004-01-01

This paper describes the first results and preliminary analysis obtained with several alignment monitoring systems recently installed in the PEP-II interaction region. The hydrostatic level system, stretched wire system, and laser tracker have been installed in addition to the existing tiltmeters and LVDT sensors. These systems detected motion of the left raft, which correlated primarily with the low energy ring (LER) current. The motion is of the order of 120 micrometers. The cause was identified as synchrotron radiation heating the beampipe, causing its expansion which then results in its deformation and offset of the IR quadrupoles. We also discuss further plans on measurements, analysis and means to counteract this motion

rf reference line for PEP

International Nuclear Information System (INIS)

Schwarz, H.D.; Weaver, J.N.

1979-03-01

A rf phase reference line in 6 segments around the 2200 meter circumference PEP storage ring is described. Each segment of the reference line is phase stabilized by its own independent feedback system, which uses an amplitude modulated reflection from the end of each line. The modulation is kept small and decoupled from the next segment to avoid crosstalk and significant modulation of the rf drive signal. An error evaluation of the system is made. The technical implementation and prototype performance are described. Prototype tests indicate that the phase error around the ring can be held below 1 degree with this relatively simple system

rf reference line for PEP

Energy Technology Data Exchange (ETDEWEB)

Schwarz, H.D.; Weaver, J.N.

1979-03-01

A rf phase reference line in 6 segments around the 2200 meter circumference PEP storage ring is described. Each segment of the reference line is phase stabilized by its own independent feedback system, which uses an amplitude modulated reflection from the end of each line. The modulation is kept small and decoupled from the next segment to avoid crosstalk and significant modulation of the rf drive signal. An error evaluation of the system is made. The technical implementation and prototype performance are described. Prototype tests indicate that the phase error around the ring can be held below 1 degree with this relatively simple system.

An asymmetric B-meson factory at PEP

International Nuclear Information System (INIS)

Garren, A.; Chattopadhyay, S.; Chin, Y.; Oddone, P.; Zisman, M.S.; Donald, M.; Feldman, G.; Paterson, J.M.; Rees, J.

1989-03-01

A preliminary design for a B-factory has been made using asymmetric collisions between positrons in the PEP storage ring and electrons in a new, log-energy ring. The design utilizes small-aperture, permanent-magnet quadrupoles close to the interaction point (IP). Optimization of optical and beam parameters at the IP will be discussed, as well as the lattice design of the interaction region and of the rings. 7 refs., 3 figs., 2 tabs

RF feedback simulation results for PEP-II

International Nuclear Information System (INIS)

Tighe, R.; Corredoura, P.

1995-06-01

A model of the RF feedback system for PEP-II has been developed to provide time-domain simulation and frequency-domain analysis of the complete system. The model includes the longitudinal beam dynamics, cavity fundamental resonance, feedback loops, and the nonlinear klystron operating near saturation. Transients from an ion clearing gap and a reference phase modulation from the longitudinal feedback system are also studied. Growth rates are predicted and overall system stability examined

APS [Advanced Photon Source] interests in PEP

International Nuclear Information System (INIS)

Moncton, D.E.; Shenoy, G.K.; Mills, D.M.

1987-11-01

As one of the very few high-energy electron storage rings in the world, potentially available for synchrotron radiation studies, PEP represents an opportunity to accomplish certain preconstruction R and D tasks relevant to the successful construction and operation of dedicated user facilities such as the Advanced Photon Source (APS) at Argonne. Three topical areas are discussed: Accelerator R and D, Insertion Devices (ID) R and D, and Beam Line Instrumentation R and D

Bunch-by-bunch feedback for PEP II

International Nuclear Information System (INIS)

Oxoby, G.; Claus, R.; Eisen, N.; Fox, J.; Hindi, H.; Hoeflich, J.; Olsen, J.; Sapozhnikov, L.; Linscott, I.

1993-01-01

The proposed PEP II B factory at SLAC requires a feedback to damp out longitudinal synchrotron oscillations. A time domain, downsampled, bunch-by-bunch feedback system in which each bunch is treated as an oscillator being driven by disturbances from other bunches is presented as we review the evolution of the system design. Results from a synchrotron oscillation damping experiment conducted at the SLAC/SSRL/SPEAR ring are also presented in this paper

e sup + e sup - Factories: PEP-II, KEKB, DAPHINE

CERN Document Server

Guiducci, S

2001-01-01

In 1999 two B-factories, PEP-II and KEK, and a PHI-factory, DAPHINE, started their physics experiments. A status report of the three factories is presented. A description of the interaction regions, strongly influenced by the detector requirements, and of the machine background in the detectors is presented.

Swamp plots for dynamic aperture studies of PEP-II lattices

International Nuclear Information System (INIS)

Yan, Y.T.; Irwin, J.; Cai, Y.; Chen, T.; Ritson, D.

1995-01-01

With a newly developed algorithm using resonance basis Lie generators and their evaluation with action-angle Poisson bracket maps (nPB tracking) the authors have been able to perform fast tracking for dynamic aperture studies of PEP-II lattices as well as incorporate lattice nonlinearities in beam-beam studies. They have been able to better understand the relationship between dynamic apertures and the tune shift and resonance coefficients in the generators of the one-turn maps. To obtain swamp plots (dynamic aperture vs. working point) of the PEP-II lattices, they first compute a one-turn resonance basis map for a nominal working point and then perform nPB tracking by switching the working point while holding fixed all other terms in the map. Results have been spot-checked by comparing with element-by-element tracking

Micellization of symmetric PEP-PEO block copolymers in water molecular weight dependence

CERN Document Server

Kaya, H; Allgaier, J; Stellbrink, J; Richter, D

2002-01-01

The micellar behaviour of the amphiphilic block copolymer poly-(ethylene-propylene)-poly-(ethylene oxide) (PEP-PEO) in aqueous solution has been studied with small-angle neutron scattering. The polymer was studied over a wide range of molecular weights, always keeping the volume of the blocks equal. The scattering behaviour of the solutions showed that a morphological transition takes place upon lowering the molecular weight. The high molecular weight block copolymers all build spherical, monodisperse micelles with large aggregation numbers. At low molecular weights, however, cylindrical micelles are formed. An interesting intermediate case is represented by the PEP2-PEO2 system, in which a morphological transition occurs upon dilution. (orig.)

Radiosynthesis of a new PSMA targeting ligand ([18F]FPy-DUPA-Pep)

International Nuclear Information System (INIS)

Malik, Noeen; Machulla, Hans-Juergen; Solbach, Christoph; Winter, Gordon; Reske, Sven N.; Zlatopolskiy, Boris

2011-01-01

Due to the specificity of expression of PSMA (prostate specific membrane antigen) particularly in prostate cancer cells (e.g. LNCaP), numerous PSMA ligands have been synthesized until now. In the current study, we synthesized DUPA-Pep having 2-[3-(1,3-dicarboxypropyl)ureido]pentanedioic acid (DUPA) linked via 8-aminooctanoic acid to two phenylalanine residues and chose 6-[ 18 F]fluoronicotinic acid 2,3,5,6-tetrafluorophenyl ester [ 18 F]FPy-TFP as a prosthetic group for coupling. [ 18 F]FPy-DUPA-Pep was obtained in a radiochemical yield of 48±0.9% (decay uncorrected) within 50 min with a chemical purity of >98%.

Vesicles mimicking normal and cancer cell membranes exhibit differential responses to the cell-penetrating peptide Pep-1.

Science.gov (United States)

Almarwani, Bashiyar; Phambu, Esther Nzuzi; Alexander, Christopher; Nguyen, Ha Aimee T; Phambu, Nsoki; Sunda-Meya, Anderson

2018-06-01

The cell-penetrating peptide (CPP) Pep-1 presents a great potential in drug delivery due to its intrinsic property to cross plasma membrane. However, its mechanism of entry into the cell remains unresolved. In this study, we compare the selectivity of Pep-1 towards vesicles mimicking normal and cancer cell membranes. The interaction was performed in a wide range of peptide-to-lipid molar ratios using infrared (IR), fluorescence, scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) techniques. At low peptide concentration, fluorescence experiments show that lipid-phosphatidylserine (PS) seems to enable Pep-1 translocation into cancer cell membrane as evidenced by the blue shift of its maximal emission wavelength. DSC data show that Pep-1 induces segregation of lipids. At high peptide concentration, IR data indicate that the interaction of Pep-1 is relatively stronger with normal cell membrane than with cancer cell membrane through the phosphate groups, while the interaction is weaker with normal cell membrane than with cancer cell membrane through the carbonyl groups. TGA and DSC data reveal that vesicles of normal cell membrane are thermally more stable than vesicles of cancer cell membrane. This suggests that the additional lipid PS included in cancer cell membrane has a destabilizing effect on the membrane structure. SEM images reveal that Pep-1 form superstructures including spherical particles and fibrils in the presence of both model membranes. PS seems to enhance peptide transport across cellular membranes. The biophysical techniques in this study provide valuable insights into the properties of CPPs in drug delivery systems. Copyright © 2018 Elsevier B.V. All rights reserved.

Searches for new particles at PEP

International Nuclear Information System (INIS)

Jonker, M.J.

1986-05-01

The status of searches for new particles at the PEP storage ring is reviewed. The result of the excited electron search by MARKII is presented and a limit on the coupling strength of the e*eγ vertex is given. The search for single photons in the ASP and MAC detectors is reported and the results are used to set limits on the number of light neutrino species and, in the context of supersymmetry (SUSY) theories, are interpreted as setting simultaneous limits on the masses of the selectron and photino

Operator interface for the PEP-II low level RF control system

International Nuclear Information System (INIS)

Allison, S.; Claus, R.

1997-05-01

This paper focuses on the operational aspects of the low level RF control system being built for the PEP-II storage rings at SLAC. Subsystems requiring major operational considerations include displays for monitor and control from UNIX workstations, slow feedback loops and control sequences residing on microprocessors, and various client applications in the existing SLAC Linear Collider (SLC) control system. Since commissioning of PEP-II RF is currently in-progress, only those parts of the control system used during this phase are discussed in detail. Based on past experience with the SLC control system, it is expected that effort expended during commissioning on a solid user interface will result in smoother transition to full reliable 24-hour-a-day operation

Proposals for ORNL [Oak Ridge National Laboratory] support to Tiber LLNL [Lawrence Livermore National Laboratory

International Nuclear Information System (INIS)

Berry, L.A.; Rosenthal, M.W.; Saltmarsh, M.J.; Shannon, T.E.; Sheffield, J.

1987-01-01

This document describes the interests and capabilities of Oak Ridge National Laboratory in their proposals to support the Lawrence Livermore National Laboratory (LLNL) Engineering Test Reactor (ETR) project. Five individual proposals are cataloged separately. (FI)

Radiosynthesis of a new PSMA targeting ligand ([18F]FPy-DUPA-Pep).

Science.gov (United States)

Malik, Noeen; Machulla, Hans-Jürgen; Solbach, Christoph; Winter, Gordon; Reske, Sven N; Zlatopolskiy, Boris

2011-07-01

Due to the specificity of expression of PSMA (prostate specific membrane antigen) particularly in prostate cancer cells (e.g. LNCaP), numerous PSMA ligands have been synthesized until now. In the current study, we synthesized DUPA-Pep having 2-[3-(1,3-dicarboxypropyl)ureido]pentanedioic acid (DUPA) linked via 8-aminooctanoic acid to two phenylalanine residues and chose 6-[(18)F]fluoronicotinic acid 2,3,5,6-tetrafluorophenyl ester [(18)F]FPy-TFP as a prosthetic group for coupling. [(18)F]FPy-DUPA-Pep was obtained in a radiochemical yield of 48±0.9% (decay uncorrected) within 50 min with a chemical purity of >98%. Copyright © 2011 Elsevier Ltd. All rights reserved.

Letter of intent for the study of CP violation and heavy flavor physics at PEP-II

Energy Technology Data Exchange (ETDEWEB)

BaBar Collaboration

1994-06-18

This report discusses the following topics on CP violation and heavy flavor physics experiments: Physics at PEP-II; detector overview; PEP-II and the interaction region; vertex detector; main tracking chamber; particle identification; electromagnetic calorimeter; muon and neutral hadron detector; magnet coil and flux return; electronics, trigger, and data acquisition; computing; CP asymmetry simulations; collaboration issues; project organization and management; and budget and schedule.

Letter of intent for the study of CP violation and heavy flavor physics at PEP-II

International Nuclear Information System (INIS)

1994-01-01

This report discusses the following topics on CP violation and heavy flavor physics experiments: Physics at PEP-II; detector overview; PEP-II and the interaction region; vertex detector; main tracking chamber; particle identification; electromagnetic calorimeter; muon and neutral hadron detector; magnet coil and flux return; electronics, trigger, and data acquisition; computing; CP asymmetry simulations; collaboration issues; project organization and management; and budget and schedule

PEP-II Beta Beat Fixes with MIA

International Nuclear Information System (INIS)

Yan, Y

2004-01-01

We first find a virtual accelerator for the HER or the LER through determination of all quadrupole strengths and sextupole feed-downs as well as all BPM gains and BPM cross-plane couplings by an SVD-enhanced Least-squares fitting of the quantities derivable from a complete set of orbits. These quantities are the phase advances, the Green's functions, and the tilt angles and axis ratios of the coupling eigen ellipses. They are obtained by analyzing turn-by-turn Beam Position Monitor (BPM) data with a high-resolution model-independent analysis (MIA). Once the virtual accelerator is found, we select a limited number of key quadrupoles, for example, the linear trombone quadrupoles and the global skews, for Least-square fitting of their strengths to minimize the beta beat while keeping other optics characters unchanged if not improved. We then dial in these limited number of quadrupole strength changes to the real accelerator (HER or LER) to achieve a better-performance PEP-II. Noticeable achievement by this MIA technique has been that MIA has helped PEP-II achieve its breaking record peak luminosity of 6.5 x 10 33 cm -2 s -1 in 2003 by bringing the LER working tune to near half integer and simultaneously fixing the beta beat, which would, otherwise, be difficult without MIA because of the strong LER coupling effect

Terapêutica da sarcoidose: importância da avaliação da PEP

Directory of Open Access Journals (Sweden)

Ema Sacadura Leite

1998-01-01

Full Text Available RESUMO: A clearance do DTPA marcado com 99mTc pode ser um método não invasivo para determinar a Permeabilidade Epitelial Pulmonar (PEP. Os objectivos do nosso estudo consistiram em avaliar a PEP de doentes com sarcoidose por aquele método e: 1 Correlacionar o seu valor com os testes funcionais respiratórios – capacidade vital (CV, volume expiratório máximo no primeiro segundo (VEMS, compliance pulmonar estática (St Cpl, difusão de monóxido de carbono (DLCO e pressão arterial de oxigénio (PaO2 com a TAC, com a fixação pulmonar de Gallium 67 e com o valor de enzima conversora de angiotensina (ECA; 2 Avaliar sea PEP poderá preverá evolução da doença pulmonar, podendo ser, nessa perspectiva, um indicador útil a ter em conta na decisão de tratar a sarcoidose pulmonar.Estudámos 34 doentes com sarcoidose confirmada por biópsia (idade média 43.3±10.9, 27/34 do sexo feminino, 20% estadio 0, 23% estadio 1, 26% estadio n e 31% estadio III ou IV, tempo de evolução da doença 3. 7±5.4 anos. Os doentes foram divididos em dois grupos: a com PEP normal – grupo 0 (n=14, b com PEP aumentada – grupo 1 (n=20. O teste Anova e o teste de Fisher foram utilizados para testar 1. A analise da covariância e teste de Mc Nemar foram utilizados para testar 2.Encontrámos uma correlação estatisticamente significativa entre os valores da PEP e a CV (G0=102.6±18.3vsG1=86.4±19.7, p=0.01, FEV1, (G0=105.4±20.5 vs G1=85.8±21.5, p=0.0 19, DLCO (G0=100.4 19.4 vs G1=83.2±22.2, p=0.02 e cintigrafia pulmonar com Gallium 67 positiva (p=0.05. Os nossos resultados sugeriram ainda que a PEP poderá prever a evolução da compliance pulmonar estática (p=0.069.Parece assim que a determinação da PEP através da clearance de99mTc – DTPA possivelmente será útil na decisão clínica de iniciar (ou reiniciar tratamento, em doentes com

ELAN - expert system supported information and management system for analytical laboratories

International Nuclear Information System (INIS)

Jaeschke, A.; Orth, H.; Zilly, G.

1990-08-01

The demand for high efficiency and short response time calls for the use of computer support in chemico-analytical laboratories. This is usually achieved by laboratory information and management systems covering the three levels of analytical instrument automation, laboratory operation support and laboratory management. The management component of the systems implemented up to now suffers from a lack of flexibility as far as unforeseen analytical investigations outside the laboratory routine work are concerned. Another drawback is the lack of adaptability with respect to structural changes in laboratory organization. It can be eliminated by the application of expert system structures and methods for the implementation of this system level. The ELAN laboratory information and management system has been developed on the basis of this concept. (orig.) [de

Effects of a school-based sexuality education program on peer educators: the Teen PEP model

OpenAIRE

Jennings, J. M.; Howard, S.; Perotte, C. L.

2014-01-01

This study evaluated the impact of the Teen Prevention Education Program (Teen PEP), a peer-led sexuality education program designed to prevent unintended pregnancy and sexually transmitted infections (STIs) including HIV among high school students. The study design was a quasi-experimental, nonrandomized design conducted from May 2007 to May 2008. The sample consisted of 96 intervention (i.e. Teen PEP peer educators) and 61 comparison students from five high schools in New Jersey. Baseline a...

Development and characterization of an exposure platform suitable for physico-chemical, morphological and toxicological characterization of printer-emitted particles (PEPs).

Science.gov (United States)

Pirela, Sandra V; Pyrgiotakis, Georgios; Bello, Dhimiter; Thomas, Treye; Castranova, Vincent; Demokritou, Philip

2014-06-01

An association between laser printer use and emissions of particulate matter (PM), ozone and volatile organic compounds has been reported in recent studies. However, the detailed physico-chemical, morphological and toxicological characterization of these printer-emitted particles (PEPs) and possible incorporation of engineered nanomaterials into toner formulations remain largely unknown. In this study, a printer exposure generation system suitable for the physico-chemical, morphological, and toxicological characterization of PEPs was developed and used to assess the properties of PEPs from the use of commercially available laser printers. The system consists of a glovebox type environmental chamber for uninterrupted printer operation, real-time and time-integrated particle sampling instrumentation for the size fractionation and sampling of PEPs and an exposure chamber for inhalation toxicological studies. Eleven commonly used laser printers were evaluated and ranked based on their PM emission profiles. Results show PM peak emissions are brand independent and varied between 3000 to 1 300 000 particles/cm³, with modal diameters ranging from 49 to 208 nm, with the majority of PEPs in the nanoscale (printer toner) raises questions about health implications to users. The presented PEGS platform will help in assessing the toxicological profile of PEPs and the link to the physico-chemical and morphological properties of emitted PM and toner formulations.

PEP-II RF feedback system simulation

Energy Technology Data Exchange (ETDEWEB)

Tighe, R [Stanford Linear Accelerator Center, Menlo Park, CA (United States)

1996-08-01

A model containing the fundamental impedance of the PEP-II cavity along with the longitudinal beam dynamics and RF feedback system components is in use. It is prepared in a format allowing time-domain as well as frequency-domain analysis and full graphics capability. Matlab and Simulink are control system design and analysis programs (widely available) with many built-in tools. The model allows the use of compiled C-code modules for compute intensive portions. We desire to represent as nearly as possible the components of the feedback system including all delays, sample rates and applicable nonlinearities. (author)

Commissioning experience with the PEP-II low-level RF system

International Nuclear Information System (INIS)

Corredoura, P.; Allison, S.; Claus, R.; Ross, W.; Sapozhnikov, L.; Schwarz, H.D.; Tighe, R.; Yee, C.; Ziomek, C.

1997-05-01

The low-level RF system for PEP-II is a modular design housed in a VXI environment and supported by EPICS. All signal processing and control is done at baseband using in-phase and quadrature (IQ) techniques. Remotely configurable RF feedback loops are used to control coupled-bunch instabilities driven by the accelerating mode of the RF cavities. A programmable DSP based feedback loop is implemented to control phase variations across the klystron due to the required adjustment of the cathode voltage to limit cathode power dissipation. The DSP loop also adaptively cancels modulations caused by klystron power supply ripple at selected power line harmonics between 60 Hz and 10 kHz. The system contains a built-in baseband network analyzer which allows remote measurement of the RF feedback loop transfer functions and automated configuration of these loops. This paper presents observations and measured data from the system

Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice

International Nuclear Information System (INIS)

Ahn, Eun Hee; Kim, Dae Won; Kang, Hye Won; Shin, Min Jae; Won, Moo Ho; Kim, Joon; Kim, Dong Joon; Kwon, Oh-Shin; Kang, Tae-Cheon; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

2010-01-01

This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.

Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling

KAUST Repository

Lori, M.; van Verk, M. C.; Hander, T.; Schatowitz, H.; Klauser, D.; Flury, P.; Gehring, Christoph A; Boller, T.; Bartels, S.

2015-01-01

Plant elicitor peptides (Peps) are potent inducers of pattern-triggered immunity and amplify the immune response against diverse pathogens. Peps have been discovered and studied extensively in Arabidopsis and only recently orthologs in maize were

Radiosynthesis of a new PSMA targeting ligand ([{sup 18}F]FPy-DUPA-Pep)

Energy Technology Data Exchange (ETDEWEB)

Malik, Noeen, E-mail: noeen.malik@uniklinik-ulm.d [Clinic for Nuclear Medicine, University Hospital, Ulm (Germany); Machulla, Hans-Juergen; Solbach, Christoph; Winter, Gordon; Reske, Sven N.; Zlatopolskiy, Boris [Clinic for Nuclear Medicine, University Hospital, Ulm (Germany)

2011-07-15

Due to the specificity of expression of PSMA (prostate specific membrane antigen) particularly in prostate cancer cells (e.g. LNCaP), numerous PSMA ligands have been synthesized until now. In the current study, we synthesized DUPA-Pep having 2-[3-(1,3-dicarboxypropyl)ureido]pentanedioic acid (DUPA) linked via 8-aminooctanoic acid to two phenylalanine residues and chose 6-[{sup 18}F]fluoronicotinic acid 2,3,5,6-tetrafluorophenyl ester [{sup 18}F]FPy-TFP as a prosthetic group for coupling. [{sup 18}F]FPy-DUPA-Pep was obtained in a radiochemical yield of 48{+-}0.9% (decay uncorrected) within 50 min with a chemical purity of >98%.

PEP-1-SIRT2 inhibits inflammatory response and oxidative stress-induced cell death via expression of antioxidant enzymes in murine macrophages.

Science.gov (United States)

Kim, Mi Jin; Kim, Dae Won; Park, Jung Hwan; Kim, Sang Jin; Lee, Chi Hern; Yong, Ji In; Ryu, Eun Ji; Cho, Su Bin; Yeo, Hyeon Ji; Hyeon, Jiye; Cho, Sung-Woo; Kim, Duk-Soo; Son, Ora; Park, Jinseu; Han, Kyu Hyung; Cho, Yoon Shin; Eum, Won Sik; Choi, Soo Young

2013-10-01

Sirtuin 2 (SIRT2), a member of the sirtuin family of proteins, plays an important role in cell survival. However, the biological function of SIRT2 protein is unclear with respect to inflammation and oxidative stress. In this study, we examined the protective effects of SIRT2 on inflammation and oxidative stress-induced cell damage using a cell permeative PEP-1-SIRT2 protein. Purified PEP-1-SIRT2 was transduced into RAW 264.7 cells in a time- and dose-dependent manner and protected against lipopolysaccharide- and hydrogen peroxide (H₂O₂)-induced cell death and cytotoxicity. Also, transduced PEP-1-SIRT2 significantly inhibited the expression of cytokines as well as the activation of NF-κB and mitogen-activated protein kinases (MAPKs). In addition, PEP-1-SIRT2 decreased cellular levels of reactive oxygen species (ROS) and of cleaved caspase-3, whereas it elevated the expression of antioxidant enzymes such as MnSOD, catalase, and glutathione peroxidase. Furthermore, topical application of PEP-1-SIRT2 to 12-O-tetradecanoylphorbol 13-acetate-treated mouse ears markedly inhibited expression levels of COX-2 and proinflammatory cytokines as well as the activation of NF-κB and MAPKs. These results demonstrate that PEP-1-SIRT2 inhibits inflammation and oxidative stress by reducing the levels of expression of cytokines and ROS, suggesting that PEP-1-SIRT2 may be a potential therapeutic agent for various disorders related to ROS, including skin inflammation. Copyright © 2013 Elsevier Inc. All rights reserved.

Transduced PEP-1-PON1 proteins regulate microglial activation and dopaminergic neuronal death in a Parkinson's disease model.

Science.gov (United States)

Kim, Mi Jin; Park, Meeyoung; Kim, Dae Won; Shin, Min Jea; Son, Ora; Jo, Hyo Sang; Yeo, Hyeon Ji; Cho, Su Bin; Park, Jung Hwan; Lee, Chi Hern; Kim, Duk-Soo; Kwon, Oh-Shin; Kim, Joon; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik; Choi, Soo Young

2015-09-01

Parkinson's disease (PD) is an oxidative stress-mediated neurodegenerative disorder caused by selective dopaminergic neuronal death in the midbrain substantia nigra. Paraoxonase 1 (PON1) is a potent inhibitor of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) against oxidation by destroying biologically active phospholipids with potential protective effects against oxidative stress-induced inflammatory disorders. In a previous study, we constructed protein transduction domain (PTD) fusion PEP-1-PON1 protein to transduce PON1 into cells and tissue. In this study, we examined the role of transduced PEP-1-PON1 protein in repressing oxidative stress-mediated inflammatory response in microglial BV2 cells after exposure to lipopolysaccharide (LPS). Moreover, we identified the functions of transduced PEP-1-PON1 proteins which include, mitigating mitochondrial damage, decreasing reactive oxidative species (ROS) production, matrix metalloproteinase-9 (MMP-9) expression and protecting against 1-methyl-4-phenylpyridinium (MPP(+))-induced neurotoxicity in SH-SY5Y cells. Furthermore, transduced PEP-1-PON1 protein reduced MMP-9 expression and protected against dopaminergic neuronal cell death in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice model. Taken together, these results suggest a promising therapeutic application of PEP-1-PON1 proteins against PD and other inflammation and oxidative stress-related neuronal diseases. Copyright © 2015 Elsevier Ltd. All rights reserved.

Measurement of the leptonic structure functions of the photon at PEP [Positron Electron Project

International Nuclear Information System (INIS)

Cain, M.P.

1987-01-01

At beam energies available at the PEP e + e - storage ring at the Stanford Linear Accelerator Center the cross-section for two-photon particle production is sufficiently large to warrant an investigation of this O(α 4 ) process. Of particular interest is the two-photon process ee → eeμμ at non-zero Q 2 . This channel is not only relatively easy to observe experimentally but also serves as a model for the process ee → eeq bar q. For the case of inelastic eγ scattering the cross-section could be parameterized in terms of the photon structure functions F 1 (x,Q 2 ) and F 2 (x,Q 2 ). In this thesis I will present data on the process ee → eeμμ collected by the Two-Photon collaboration (PEP-9) at PEP. For the subset of data which proceeds by inelastic eγ scattering I will present a procedure for extracting the QED photon structure functions and apply this method to the data. 42 refs., 44 figs

Effect of the Electron Cloud and CSR on the Upgrade of the PEP-II

International Nuclear Information System (INIS)

Heifets, Samuel A

2001-01-01

Effects of the electron cloud and of the coherent synchrotron radiation (CSR) on the possible upgrade of the PEP-II B-factory are studied. PEP-II B factory operates with parameters shown in Table 1 and already exceeds the design luminosity. Nevertheless, a possibility of upgrading the machine to even higher luminosities is under consideration [1]. Several scenarios are summarized in Table 2. This paper describes effects of the electron cloud and of the coherent synchrotron radiation (CSR) on the proposed upgrades of the PEP-II B-factory. The first effect was observed [3] and caused [4] the degradation of the emittance at KEK B-factory. The analytic expression for the e-wake [2] is used in calculations of the head-tail instability. Other obvious effects of higher beam currents such as additional heat load are not considered. The short wave length CSR has been recently observed at Brookhaven [6]. Consideration of the effect of such CSR on the beam dynamics is based on our previous paper [7

The PEP [positron-electron-proton] electron-positron ring: An update

International Nuclear Information System (INIS)

1975-03-01

The first stage of the positron-electron-protron (PEP) colliding-beam system, which has been under joint study by a Lawrence Berkeley Laboratory--Stanford Linear Accelerator Center team for the past two years, will be the electron-positron storage ring. The physics justification for the e + e/sup /minus// ring is summarized briefly and the proposed facility is described. The ring will have six arcs having gross radii of about 220 m and six interaction regions located at the centers of straight sections about 130 m long. The longitudinal distance left free for experimental apparatus around the interaction regions was set provisionally at 20 m. The range of operating beam energies will be from 5 GeV to 15 GeV. The design luminosity at 15 GeV will be 10 32 cm/sup /minus/2/s/sup /minus/1/, and the luminosity will vary approximately as the square of the beam energy. Alternative methods under consideration for adjusting the beam cross section are discussed. The designs of the storage ring subsystems and of the conventional facilities including the experimental halls at the interaction regions are described. 7 figs., 3 tabs

Performance evaluation of PEP

International Nuclear Information System (INIS)

Zisman, M.S.

1987-10-01

An investigation of collective effects has been undertaken to assess the possibilities for using the low emittance operating mode of the PEP storage ring as a dedicated source of synchrotron radiation. Beam current limitations associated with longitudinal and transverse instabilities, and the expected emittance growth due to intrabeam scattering have been studied as a function of beam energy. Calculations of the beam lifetime due to Touschek and gas scattering are presented, and the growth times of coupled-bunch instabilities are estimated. In general, the results are encouraging, and no fundamental problems have been uncovered. It appears that beam currents up to about 10 mA per bunch should be achievable, and that the emittance growth is not a severe problem at an energy of about 8 GeV. A feedback system to deal with coupled-bunch instabilities is likely to be required. 7 refs., 13 figs

Status of the PEP-II asymmetric B-Factory

International Nuclear Information System (INIS)

Mattison, T.S.

1994-11-01

The PEP-II project is an e + e - storage ring complex with unequal energy beams to study CP violation in the B meson system. High luminosity requires a large number of bunches and a low value of beta at the collision point. The high beam current requires advances in vacuum chambers, damped RF cavities, and feedback systems. Machine elements inside the detector are required to achieve low beta values and magnetic separation

Measuring, calculating and estimating PEP's parasitic mode loss parameters

International Nuclear Information System (INIS)

Weaver, J.N.

1981-01-01

This note discusses various ways the parasitic mode losses from a bunched beam to a vacuum chamber can be measured, calculated or estimated. A listing of the parameter, k, for the various PEP ring components is included. A number of formulas for calculating multiple and single pass losses are discussed and evaluated for several cases. 25 refs., 1 fig., 1 tab

Preliminary design considerations for the stage 1 PEP lattice

International Nuclear Information System (INIS)

Helm, R.H.; Lee, M.J.

1974-07-01

A general description of the proposed PEP e + e - storage ring is discussed in the paper. We discuss the lattice and its operating characteristics in more detail, show how the design luminosity operative regions may be met and outline the limits of the operative regions of the beam parameters in several modes of operation. 18 refs., 16 figs., 1 tab

PEP-II vacuum system pressure profile modeling using EXCEL

International Nuclear Information System (INIS)

Nordby, M.; Perkins, C.

1994-06-01

A generic, adaptable Microsoft EXCEL program to simulate molecular flow in beam line vacuum systems is introduced. Modeling using finite-element approximation of the governing differential equation is discussed, as well as error estimation and program capabilities. The ease of use and flexibility of the spreadsheet-based program is demonstrated. PEP-II vacuum system models are reviewed and compared with analytical models

Mesomorphic phase behaviour of low molar mass PEP-PDMS diblock copolymers synthesized by anionic polymerization

International Nuclear Information System (INIS)

Vigild, M.E.

1997-10-01

The phase behaviour of low molar mass poly(ethylene-alt-propylene) -poly(dimethylsiloxane) (PEP-PDMS) is investigated in this thesis by the combination of dynamical mechanical spectroscopy (rheology) to measure phase transition temperatures, and small-angle x-ray scattering to identify the morphology of encountered phases. Samples of PEP-PDMS in the range of 0.2-0.7 in volume fraction of PEP are studied. This diblock copolymer system exhibits the three classical phases of lamellar sandwich structure (LAM), hexagonally packed cylinders (HEX), and spheres arranged on a body centered cubic lattice (BCC). Furthermore the gyroid phase (Ia3d symmetry) of two interpenetrating networks was also identified as a stable phase of the PEP-PDMS system. Time resolved measurements of small-angle neutron scattering in tandem with simultaneous in-situ rheological measurements are performed on samples showing transitions between different ordered phases. The identification of especially the BCC and gyroid phases from scattering experiments is treated. By performing mesoscopic crystallographic measurements using a custom built goniometer it was unambiguously shown that the application of shear to an unoriented powder-like sample introduces uniaxial orientation of the gyroid phase. The orientation of the ordered phase is otherwise random, causing a two-dimensional powder. Finally this dissertation presents a discussion of relevant parameters for the description of diblock copolymer phase behaviour together with descriptions of anionic polymerization for the synthesis of copolymers, and various experimental techniques for the characterization of diblocks. (au)

Mesomorphic phase behaviour of low molar mass PEP-PDMS diblock copolymers synthesized by anionic polymerization

Energy Technology Data Exchange (ETDEWEB)

Vigild, M.E.

1997-10-01

The phase behaviour of low molar mass poly(ethylene-alt-propylene) -poly(dimethylsiloxane) (PEP-PDMS) is investigated in this thesis by the combination of dynamical mechanical spectroscopy (rheology) to measure phase transition temperatures, and small-angle x-ray scattering to identify the morphology of encountered phases. Samples of PEP-PDMS in the range of 0.2-0.7 in volume fraction of PEP are studied. This diblock copolymer system exhibits the three classical phases of lamellar sandwich structure (LAM), hexagonally packed cylinders (HEX), and spheres arranged on a body centered cubic lattice (BCC). Furthermore the gyroid phase (Ia3d symmetry) of two interpenetrating networks was also identified as a stable phase of the PEP-PDMS system. Time resolved measurements of small-angle neutron scattering in tandem with simultaneous in-situ rheological measurements are performed on samples showing transitions between different ordered phases. The identification of especially the BCC and gyroid phases from scattering experiments is treated. By performing mesoscopic crystallographic measurements using a custom built goniometer it was unambiguously shown that the application of shear to an unoriented powder-like sample introduces uniaxial orientation of the gyroid phase. The orientation of the ordered phase is otherwise random, causing a two-dimensional powder. Finally this dissertation presents a discussion of relevant parameters for the description of diblock copolymer phase behaviour together with descriptions of anionic polymerization for the synthesis of copolymers, and various experimental techniques for the characterization of diblocks. (au). 9 tabs., 40 ills., 81 refs.

Impedance of the PEP-II DIP screen

Energy Technology Data Exchange (ETDEWEB)

Ng, C -K [Stanford Linear Accelerator Center, Menlo Park, CA (United States); Weiland, T

1996-08-01

The vacuum chamber of a storage ring normally consists of periodically spaced pumping slots. The longitudinal impedance of slots are analyzed in this paper. It is found that although the broad-band impedance is tolerable, the narrow-band impedance, as a consequence of the periodicity of the slots, may exceed the stability limit given by natural damping with no feedback system on. Based on this analysis, the PEP-II distributed-ion-pump (DIP) screen uses long grooves with hidden holes cut halfway to reduce both the broad-band and narrow-band impedances. (author)

Search for heavy neutrino production at PEP

International Nuclear Information System (INIS)

Feldman, G.J.

1985-10-01

We report a search for long-lived heavy neutrinos produced by the neutral weak current in e + e - annihilation at 29 GeV at PEP. Data from the Mark II detector are examined for evidence of events with one or two separated vertices in the radial range of 2 mm to 10 cm. No events were found that were consistent with the hypothesis of heavy neutrino production, eliminating the possibility of heavy neutrinos with decay lengths of 1 to 20 cm in mass range 1 to 13 GeV/c 2

Impedance of the PEP-II DIP screen

International Nuclear Information System (INIS)

Ng, C.K.; Weiland, T.

1995-09-01

The vacuum chamber of a storage ring normally consists of periodically spaced pumping slots. The longitudinal impedance of slots are analyzed in this paper. It is found that although the broad-band impedance is tolerable, the narrow-band impedance, as a consequence of the periodicity of the slots, may exceed the stability limit given by natural damping with no feedback system on. Based on this analysis, the PEP-II distributed-ion-pump (DIP) screen uses long grooves with hidden holes cut halfway to reduce both the broad-band and narrow-band impedances

Tests of μ-e universality for weak neutral currents at PEP

International Nuclear Information System (INIS)

Cline, D.B.; Resvanis, L.K.

1988-01-01

Two techniques are proposed to test the universality of the μ/minus/e weak neutral current interaction of large Q 2 . Both techniques require large statistics and some degree of longitudinal e + ,e/sup minus/ polarization but are otherwise feasible at PEP

Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors : interfamily incompatibility of perception but compatibility of downstream signalling

NARCIS (Netherlands)

Lori, Martina; van Verk, Marcel C; Hander, Tim; Schatowitz, Hendrik; Klauser, Dominik; Flury, Pascale; Gehring, Christoph A; Boller, Thomas; Bartels, Sebastian

2015-01-01

Plant elicitor peptides (Peps) are potent inducers of pattern-triggered immunity and amplify the immune response against diverse pathogens. Peps have been discovered and studied extensively in Arabidopsis and only recently orthologs in maize were also identified and characterized in more detail.

Optimization of Dynamic Aperture of PEP-X Baseline Design

Energy Technology Data Exchange (ETDEWEB)

Wang, Min-Huey; /SLAC; Cai, Yunhai; /SLAC; Nosochkov, Yuri; /SLAC

2010-08-23

SLAC is developing a long-range plan to transfer the evolving scientific programs at SSRL from the SPEAR3 light source to a much higher performing photon source. Storage ring design is one of the possibilities that would be housed in the 2.2-km PEP-II tunnel. The design goal of PEPX storage ring is to approach an optimal light source design with horizontal emittance less than 100 pm and vertical emittance of 8 pm to reach the diffraction limit of 1-{angstrom} x-ray. The low emittance design requires a lattice with strong focusing leading to high natural chromaticity and therefore to strong sextupoles. The latter caused reduction of dynamic aperture. The dynamic aperture requirement for horizontal injection at injection point is about 10 mm. In order to achieve the desired dynamic aperture the transverse non-linearity of PEP-X is studied. The program LEGO is used to simulate the particle motion. The technique of frequency map is used to analyze the nonlinear behavior. The effect of the non-linearity is tried to minimize at the given constrains of limited space. The details and results of dynamic aperture optimization are discussed in this paper.

Optimization of Dynamic Aperture of PEP-X Baseline Design

International Nuclear Information System (INIS)

Wang, Min-Huey

2010-01-01

SLAC is developing a long-range plan to transfer the evolving scientific programs at SSRL from the SPEAR3 light source to a much higher performing photon source. Storage ring design is one of the possibilities that would be housed in the 2.2-km PEP-II tunnel. The design goal of PEPX storage ring is to approach an optimal light source design with horizontal emittance less than 100 pm and vertical emittance of 8 pm to reach the diffraction limit of 1-(angstrom) x-ray. The low emittance design requires a lattice with strong focusing leading to high natural chromaticity and therefore to strong sextupoles. The latter caused reduction of dynamic aperture. The dynamic aperture requirement for horizontal injection at injection point is about 10 mm. In order to achieve the desired dynamic aperture the transverse non-linearity of PEP-X is studied. The program LEGO is used to simulate the particle motion. The technique of frequency map is used to analyze the nonlinear behavior. The effect of the non-linearity is tried to minimize at the given constrains of limited space. The details and results of dynamic aperture optimization are discussed in this paper.

Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry.

Science.gov (United States)

Pavlova, Ivelina; Milanova, Aneliya; Danova, Svetla; Fink-Gremmels, Johanna

2016-12-01

Expression of peptide transporter 1 (PepT1) and liver-expressed antimicrobial peptide 2 (LEAP-2) in chickens can be influenced by food deprivation, pathological conditions and drug administration. Effect of three putative probiotic Lactobacillus strains and enrofloxacin on the expression of PepT1 and LEAP-2 mRNA was investigated in Ross 308 chickens. One-day-old chicks (n = 24) were allocated to following groups: control (without treatment); group treated with probiotics via feed; group treated with a combination of probiotics and enrofloxacin; and a group given enrofloxacin only. The drug was administered at a dose of 10 mg kg -1 , via drinking water for 5 days. Samples from liver, duodenum and jejunum were collected 126 h after the start of the treatment. Expression levels of PepT1 and LEAP-2 were determined by real-time polymerase chain reaction and were statistically evaluated by Mann-Whitney test. Enrofloxacin administered alone or in combination with probiotics provoked a statistically significant up-regulation of PepT1 mRNA levels in the measured organ sites. These changes can be attributed to a tendency of improvement in utilization of dietary peptide and in body weight gain. LEAP-2 mRNA expression levels did not change significantly in enrofloxacin-treated chickens in comparison with control group.

The PEP injection system

International Nuclear Information System (INIS)

Brown, K.L.; Avery, R.T.; Peterson, J.M.

1988-01-01

A system to transport 10-to-15-GeV electron and positron beams from the Stanford Linear Accelerator and to inject them into the PEP storage ring under a wide variety of lattice configurations has been designed. Optically, the transport line consists of three 360/degree/ phase-shift sections of FODO lattice, with bending magnets interspersed in such a way as to provide achromaticity, convenience in energy and emittance definition, and independent tuning of the various optical parameters for matching into the ring. The last 360/degree/ of phase shift has 88 milliradians of bend in a vertical plane and deposits the beam at the injection septum via a Lambertson magnet. Injection is accomplished by launching the beam with several centimeters of radial betatron amplitude in a fast bump provided by a triad of pulsed kicker magnets. Radiation damping reduces the collective amplitude quickly enough to allow injection at a high repetition rate

Can PEP-3 Provide a Cognitive Profile in Children with ASD? A Comparison between the Developmental Ages of PEP-3 and IQ of Leiter-R

Science.gov (United States)

De Giacomo, Andrea; Craig, Francesco; Cristella, Arcangelo; Terenzio, Vanessa; Buttiglione, Maura; Margari, Lucia

2016-01-01

Background: The assessment of the intelligence quotient (IQ) in children with autism spectrum disorder (ASD) is important to plan a detailed therapeutic-educative programme. The aim of the study was to evaluate the usefulness of the Psychoeducational Profile-third edition (PEP-3) to estimate the general cognitive development of children with ASD.…

PEP surveying procedures and equipment

International Nuclear Information System (INIS)

Linker, F.

1982-06-01

The PEP Survey and Alignment System, which employs both laser-based and optical survey methods, is described. The laser is operated in conjunction with the Tektronix 4051 computer and surveying instruments such as ARM and SAM, system which is designed to automate data input, reduction, and production of alignment instructions. The laser system is used when surveying ring quadrupoles, main bend magnets, sextupoles, and is optional when surveying RF cavities and insertion quadrupoles. Optical methods usually require that data be manually entered into the computer for alignment, but in some cases, an element can be aligned using nominal values of fiducial locations without use of the computer. Optical surveying is used in the alignment of NIT and SIT, low field bend magnets, wigglers, RF cavities, and insertion quadrupoles

PEP-II RF cavity revisited

International Nuclear Information System (INIS)

Rimmer, R.A.; Koehler, G.; Li, D.; Hartman, N.; Folwell, N.; Hodgson, J.; Ko, K.; McCandless, B.

1999-01-01

This report describes the results of numerical simulations of the PEP-II RF cavity performed after the completion of the construction phase of the project and comparisons are made to previous calculations and measured results. These analyses were performed to evaluate new calculation techniques for the HOM distribution and RF surface heating that were not available at the time of the original design. These include the use of a high frequency electromagnetic element in ANSYS and the new Omega 3P code to study wall losses, and the development of broadband time domain simulation methods in MAFIA for the HOM loading. The computed HOM spectrum is compared with cavity measurements and observed beam-induced signals. The cavity fabrication method is reviewed, with the benefit of hindsight, and simplifications are discussed

HyPEP-FY 07 Annual Report: A Hydrogen Production Plant Efficiency Calculation Program

Energy Technology Data Exchange (ETDEWEB)

Chang Oh

2007-09-01

The Very High Temperature Gas-Cooled Reactor (VHTR) coupled to the High Temperature Steam Electrolysis (HTSE) process is one of two reference integrated systems being investigated by the U.S. Department of Energy and Idaho National Laboratory for the production of hydrogen. In this concept the VHTR outlet temperature of 900 °C provides thermal energy and high efficiency electricity for the electrolysis of steam in the HTSE process. In the second reference system the Sulfur Iodine (SI) process is coupled to the VHTR to produce hydrogen thermochemically. In the HyPEP project we are investigating and characterizing these two reference systems with respect to production, operability, and safety performance criteria. Under production, plant configuration and working fluids are being studied for their effect on efficiency. Under operability, control strategies are being developed with the goal of maintaining equipment within operating limits while meeting changes in demand. Safety studies are to investigate plant response for equipment failures. Specific objectives in FY07 were (1) to develop HyPEP Beta and verification and validation (V&V) plan, (2) to perform steady state system integration, (3) to perform parametric studies with various working fluids and power conversion unit (PCU) configurations, (4) the study of design options such as pressure, temperature, etc. (5) to develop a control strategy and (6) to perform transient analyses for plant upsets, control strategy, etc for hydrogen plant with PCU. This report describes the progress made in FY07 in each of the above areas. (1) The HyPEP code numeric scheme and Graphic User Interface have been tested and refined since the release of the alpha version a year ago. (2) The optimal size and design condition for the intermediate heat exchanger, one of the most important components for integration of the VHTR and HTSE plants, was estimated. (3) Efficiency calculations were performed for a variety of working fluids for

Background sources and masks for Mark II detector at PEP

International Nuclear Information System (INIS)

Kadyk, J.

1981-06-01

The shielding masks currently at use in several of the current experiments at PEP are the result of an early organized effort to understand the sources of particle background expected at PEP, followed by the evolution of the conceptual designs into actual hardware. The degree and kind of background particle loading which could be tolerated was expected to differ significantly among the different experiments, and several designs emerged from the common study. Qualitatively, the types of radiations studied were, Synchrotron Radiation (SR), Beam Gas Bremsstrahlung (BGB), and, to a limited extent others, e.g., Electroproduction (EP). Calculations will be given of predicted occupancies in the pipe counter and other sensitive elements at small radius, since these will be most susceptible to the SR and BGB backgrounds. The calculations presented in this note are specific to the Mark II detector. Some general statements will be made first about the character of each of the various types of backgrounds considered, then some detailed calculations made for application to the Mark II detector

Investigation of the substrate specificity of the proton coupled peptide transporter PepT_So from Shewanella oneidensis

DEFF Research Database (Denmark)

Prabhala, Bala Krishna; Aduri, Nanda Gowtham; Hald, Helle

2015-01-01

a strikingly high sequence identity, can be used to rationalize its mechanism and substrate preference. However, very little is known about the substrate specificity of PepTSo. To elaborate on this, the natural peptide specificity of PepTSo was investigated. Di and tri-peptides were found to be substrates...... for PepTSo in contrast to mono- and tetrapeptides as was indicated by previous competition studies. Interestingly, a negatively charged side chain was better accommodated on the dipeptide N- than the C-terminus position. Inversely, a positive charged side chain appeared to be tolerated better...

A zero-length bellows for the PEP-II High-Energy Ring

International Nuclear Information System (INIS)

Nordby, M.; Daly, E.F.; Kurita, N.; Langton, J.

1995-08-01

Due to the beamline space constrictions and the modular design of the vacuum system, a conventional bellows can not be used everywhere in the PEP-II High-Energy Ring (HER) arcs. A zero-length ''Flex Flange'' was developed which actually performs better than a more standard bellows. The Flex Flange fits the space available while still preserving the modularity of the system. Furthermore, the design provides for an accurate match-up between adjoining octagonal copper chambers despite the large fabrication and assembly tolerances and high operational loads. Beam chamber continuity is ensured by an integral RF seal ring which is easy to install and fault-tolerant. Heating from synchrotron radiation and higher-order mode trapping is managed to ensure a robust connection despite the 3,000 mA beam current of the PEP-II HER. The Flex Flange concept is versatile and adaptable to many applications, yet economical both in space needed and cost

Particle searches in e+e- experiments at PEP and PETRA

International Nuclear Information System (INIS)

Lau, K.H.

1982-10-01

This talk reviews recent results in new particle searches performed by experiments at the high energy e + e - storage rings PEP and PETRA. It concentrates on recent searches for: hadrons with a new quark flavor, spin-1/2 charged heavy leptons, spin-0 charged leptons, spin-0 point-like scalars or pseudoscalars, and neutral heavy leptons

Process Testing Results and Scaling for the Hanford Waste Treatment and Immobilization Plant (WTP) Pretreatment Engineering Platform - 10173

International Nuclear Information System (INIS)

Kurath, Dean E.; Daniel, Richard C.; Baldwin, David L.; Rapko, Brian M.; Barnes, Steven M.; Gilbert, Robert A.; Mahoney, Lenna A.; Huckaby, James L.

2010-01-01

The U.S. Department of Energy-Office of River Protections Hanford Tank Waste Treatment and Immobilization Plant (WTP) is being designed and built to pretreat and then vitrify a large portion of the wastes in Hanfords 177 underground waste storage tanks at Richland, Washington. In support of this effort, engineering-scale tests at the Pretreatment Engineering Platform (PEP) have been completed to confirm the process design and provide improved projections of system capacity. The PEP is a 1/4.5-scale facility designed, constructed, and operated to test the integrated leaching and ultrafiltration processes being deployed at the WTP. The PEP replicates the WTP leaching processes with prototypic equipment and control strategies and non-prototypic ancillary equipment to support the core processing. The testing approach used a nonradioactive aqueous slurry simulant to demonstrate the unit operations of caustic and oxidative leaching, cross-flow ultrafiltration solids concentration, and solids washing. Parallel tests conducted at the laboratory scale with identical simulants provided results that allow scale-up factors to be developed between the laboratory and PEP performance. This paper presents the scale-up factors determined between the laboratory and engineering-scale results and presents arguments that extend these results to the full-scale process.

RF system design for the PEP-II B Factory

International Nuclear Information System (INIS)

Schwarz, H.; Rimmer, R.

1994-06-01

The paper presents an overview of the design of the RF system for the PEP-II B Factory. An RF station consists of either two or four single-cell cavities driven by a 1.2 MW klystron through a waveguide distribution network. A variety of feedback loops stabilize the RF and its interaction with the beam. System parameters and all the relevant parameters of klystron and cavities are given

Reliable 6 PEP LTPS device for AMOLED's

Science.gov (United States)

Chou, Cheng-Wei; Wang, Pei-Yun; Hu, Chin-Wei; Chang, York; Chuang, Ching-Sang; Lin, Yusin

2013-09-01

This study presents a TFT structure which has less photo process and higher cost competitiveness in AMOLED display markets. A novel LTPS based 6 masks TFT structure for bottom emission AMOLED display is demonstrated in this paper. High field effect mobility (PMOS < 80 cm2/Vs ) and high reliability (PBTS △Vth< 0.02V @ 50oC VG=15V 10ks) was accomplished without the high temperature and rapid thermal annealing (RTA) activation process. Furthermore, a 14-inch AMOLED TV was achieved on the proposed 6-pep TFT backplane using the Gen. 3.5 mass production factory.

Radiation and electrical safety systems for PEP

International Nuclear Information System (INIS)

Smith, H.; Constant, T.; Crook, K.; Fitch, J.; Taylor, T.

1981-02-01

At SLAC, the Personnel Protection System (PPS) protects people from radiation hazards. For PEP, the system has been expanded to include protection against electrical and RF hazards. This paper describes the overall system design, giving particular attention to the novel features not found in similar systems in other areas of SLAC. These include the Restricted Access Mode to allow limited occupancy in the ring while high voltage or RF may be present, the automatic badge reader system for improving the efficiency of entry logging and control, and the solid state lighting control system for switching large lighting loads with minimum electro-magetic interference

BABAR - the detector for the PEP II B Factory at SLAC

International Nuclear Information System (INIS)

Lueth, V.

1994-09-01

BABAR refers to the detector that is being designed for the PEP II B-Factory at SLAC to perform a comprehensive study of CP violation in B meson decays. The design requirements and the principal detector components are briefly described. A summary of the expected physics performance is presented

Pep-up: A review of the Umgeni Valley Project evaulation process ...

African Journals Online (AJOL)

Pep-up: A review of the Umgeni Valley Project evaulation process. Tim Wright. Abstract. No Abstract. Full Text: EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT · Creative Commons License This work is licensed under a Creative Commons Attribution 3.0 License.

Cloning and nucleotide sequence analysis of pepV, a carnosinase gene from Lactobacillus delbrueckii subsp. lactis DSM 7290, and partial characterization of the enzyme.

Science.gov (United States)

Vongerichten, K F; Klein, J R; Matern, H; Plapp, R

1994-10-01

Cell extracts of Lactobacillus delbrueckii subsp. lactis DSM 7290 were found to exhibit unique peptolytic ability against unusual beta-alanyl-dipeptides. In order to clone the gene encoding this activity, designated pepV, a gene library of strain DSM 7290 genomic DNA, prepared in the low-copy-number plasmid pLG339, was screened for heterologous expression in Escherichia coli. Recombinant clones harbouring pepV were identified by their ability to allow the utilization of carnosine (beta-alanyl-histidine) as a source of histidine by the E. coli mutant strain UK197 (pepD, hisG). Complementation was observed in a colony harbouring a recombinant plasmid (pKV101), carrying pepV. A 2.4 kb fragment containing pepV was subcloned and its nucleotide sequence revealed an open reading frame (ORF) of 1413 nucleotides, corresponding to a protein with predicted molecular mass of 51998 Da. A single transcription initiation site 71 bp upstream of the ATG translational start codon was identified by primer extension. No significant homology was detected between pepV or its deduced amino acid sequence with any entry in the databases. The only similarity was found in a region conserved in the ArgE/DapE/CPG2/YscS family of proteins. This observation, and protease inhibitor studies, indicated that pepV is of the metalloprotease type. A second ORF present in the sequenced fragment showed extensive homology to a variety of amino acid permeases from E. coli and Saccharomyces cerevisiae.

A Parallel Controls Software Approach for PEP II: AIDA and Matlab Middle Layer

International Nuclear Information System (INIS)

Wittmer, W.; Colocho, W.; White, G.

2007-01-01

The controls software in use at PEP II (Stanford Control Program - SCP) had originally been developed in the eighties. It is very successful in routine operation but due to its internal structure it is difficult and time consuming to extend its functionality. This is problematic during machine development and when solving operational issues. Routinely, data has to be exported from the system, analyzed offline, and calculated settings have to be reimported. Since this is a manual process, it is time consuming and error-prone. Setting up automated processes, as is done for MIA (Model Independent Analysis), is also time consuming and specific to each application. Recently, there has been a trend at light sources to use MATLAB as the platform to control accelerators using a 'MATLAB Middle Layer' (MML), and so called channel access (CA) programs to communicate with the low level control system (LLCS). This has proven very successful, especially during machine development time and trouble shooting. A special CA code, named AIDA (Accelerator Independent Data Access), was developed to handle the communication between MATLAB, modern software frameworks, and the SCP. The MML had to be adapted for implementation at PEP II. Colliders differ significantly in their designs compared to light sources, which poses a challenge. PEP II is the first collider at which this implementation is being done. We will report on this effort, which is still ongoing

Study of Beam-Beam Effects at PEP-II

International Nuclear Information System (INIS)

Narsky, I

2004-01-01

Using a self-consistent three-dimensional simulation running on parallel supercomputers, we have modeled the beam-beam interaction at the PEP-II asymmetric e + e - collider. To provide guidance for luminosity improvement, we scanned the tunes and currents in both rings and computed their impact on the luminosity and transverse beam sizes. We also studied the effects of colliding the beams with a small crossing angle. Where possible, the code was benchmarked against experimental measurements of luminosity and beam sizes, yielding an acceptable agreement

PEP computer control system

International Nuclear Information System (INIS)

1979-03-01

This paper describes the design and performance of the computer system that will be used to control and monitor the PEP storage ring. Since the design is essentially complete and much of the system is operational, the system is described as it is expected to 1979. Section 1 of the paper describes the system hardware which includes the computer network, the CAMAC data I/O system, and the operator control consoles. Section 2 describes a collection of routines that provide general services to applications programs. These services include a graphics package, data base and data I/O programs, and a director programm for use in operator communication. Section 3 describes a collection of automatic and semi-automatic control programs, known as SCORE, that contain mathematical models of the ring lattice and are used to determine in real-time stable paths for changing beam configuration and energy and for orbit correction. Section 4 describes a collection of programs, known as CALI, that are used for calibration of ring elements

Background estimates for PEP

International Nuclear Information System (INIS)

Martin, F.

1975-04-01

The beam gas bremsstrahlung radiation for PEP has been studied in detail using the ray trace program TURTLE. With this program, an electron (positron) trajectory starting at an interaction region is traced through the storage ring until it radiates, then the radiated electron is traced until it stops in the beam pipe. The relative number of electrons stopping between the strong focusing quadrupoles is used to determine a rate at which electromagnetic showers will occur in the region of the experimental apparatus. It was found that 2.46% of the radiated electrons will stop in the interaction region. Assuming a uniform pressure of 2 x 10/sup /minus/9/ Torr in the vacuum pipe for a residual gas (X/sub o/ = 34.7 gm/cm 2 ), 2.5 x 10 5 electrons (positrons) will stop in the interaction region per beam (4 x 10 12 electrons at 15 GeV) per second. If the luminosity is 10 32 , this rate is equivalent to a cross section of 2.5 x 10/sup /minus/27/ cm 2 . 4 figs., 3 tabs

An Orbit And Dispersion Correction Scheme for the PEP II

International Nuclear Information System (INIS)

Cai, Y.; Donald, M.; Shoaee, H.; White, G.; Yasukawa, L.A.

2011-01-01

To achieve optimum luminosity in a storage ring it is vital to control the residual vertical dispersion. In the original PEP storage ring, a scheme to control the residual dispersion function was implemented using the ring orbit as the controlling element. The 'best' orbit not necessarily giving the lowest vertical dispersion. A similar scheme has been implemented in both the on-line control code and in the simulation code LEGO. The method involves finding the response matrices (sensitivity of orbit/dispersion at each Beam-Position-Monitor (BPM) to each orbit corrector) and solving in a least squares sense for minimum orbit, dispersion function or both. The optimum solution is usually a subset of the full least squares solution. A scheme of simultaneously correcting the orbits and dispersion has been implemented in the simulation code and on-line control system for PEP-II. The scheme is based on the eigenvector decomposition method. An important ingredient of the scheme is to choose the optimum eigenvectors that minimize the orbit, dispersion and corrector strength. Simulations indicate this to be a very effective way to control the vertical residual dispersion.

Fabrication process for the PEP II RF cavities

Energy Technology Data Exchange (ETDEWEB)

Franks, R.M.; Rimmer, R.A. [Lawrence Berkeley National Lab., CA (United States); Schwarz, H. [Stanford Linear Accelerator Center, Menlo Park, CA (United States)

1997-06-05

This paper presents the major steps used in the fabrication of the 26 RF Cavities required for the PEP-II B-factory. Several unique applications of conventional processes have been developed and successfully implemented: electron beam welding (EBW), with minimal porosity, of .75 inch (19 mm) copper cross-sections; extensive 5-axis milling of water channels; electroplating of .37 inch (10 mm) thick OFE copper; tuning of the cavity by profiling beam noses prior to final joining with the cavity body; and machining of the cavity interior, are described here.

Detecting effects of the indicated prevention Programme for Externalizing Problem behaviour (PEP) on child symptoms, parenting, and parental quality of life in a randomized controlled trial.

Science.gov (United States)

Hanisch, Charlotte; Freund-Braier, Inez; Hautmann, Christopher; Jänen, Nicola; Plück, Julia; Brix, Gabriele; Eichelberger, Ilka; Döpfner, Manfred

2010-01-01

Behavioural parent training is effective in improving child disruptive behavioural problems in preschool children by increasing parenting competence. The indicated Prevention Programme for Externalizing Problem behaviour (PEP) is a group training programme for parents and kindergarten teachers of children aged 3-6 years with externalizing behavioural problems. To evaluate the effects of PEP on child problem behaviour, parenting practices, parent-child interactions, and parental quality of life. Parents and kindergarten teachers of 155 children were randomly assigned to an intervention group (n = 91) and a nontreated control group (n = 64). They rated children's problem behaviour before and after PEP training; parents also reported on their parenting practices and quality of life. Standardized play situations were video-taped and rated for parent-child interactions, e.g. parental warmth. In the intention to treat analysis, mothers of the intervention group described less disruptive child behaviour and better parenting strategies, and showed more parental warmth during a standardized parent-child interaction. Dosage analyses confirmed these results for parents who attended at least five training sessions. Children were also rated to show less behaviour problems by their kindergarten teachers. Training effects were especially positive for parents who attended at least half of the training sessions. CBCL: Child Behaviour Checklist; CII: Coder Impressions Inventory; DASS: Depression anxiety Stress Scale; HSQ: Home-situation Questionnaire; LSS: Life Satisfaction Scale; OBDT: observed behaviour during the test; PCL: Problem Checklist; PEP: prevention programme for externalizing problem behaviour; PPC: Parent Problem Checklist; PPS: Parent Practices Scale; PS: Parenting Scale; PSBC: Problem Setting and Behaviour checklist; QJPS: Questionnaire on Judging Parental Strains; SEFS: Self-Efficacy Scale; SSC: Social Support Scale; TRF: Caregiver-Teacher Report Form.

Control electronics of the PEP RF system

International Nuclear Information System (INIS)

Pellegrin, J.L.; Schwarz, H.

1981-01-01

The operation of the major components used for controlling the phase and field level of the PEP RF cavities is described. The control electronics of one RF station is composed of several control loops: each cavity has a tuners' servo loop which maintains the frequency constant and also keeps the fields of each cavity balanced; the total gap voltage developed by a pair of cavities is regulated by a gap voltage controller; finally, the phase variation along the amplification chain, the klystron and the cavities are compensated by a phase lock loop. The design criteria of each loop are set forth and the circuit implementation and test results are presented

A first look at a polarimeter for EPIC or PEP

International Nuclear Information System (INIS)

Toner, W.T.

1988-01-01

A derivation of the resolution function is given in terms of machine parameters and detector resolution. A preliminary design is outlined for a pair spectrometer to detect high energy backscattered laser photons. A more careful examination is needed of the asymmetry as a function of resolution, of laser geometry and rates, and of problems associated with installing and using the system at PEP. 2 figs., 1 tab

RF feedback simulation for the PEP-II B Factory

International Nuclear Information System (INIS)

Tighe, R.

1994-06-01

A model, of the beam and RF system for PEP-11 has been developed to allow both time-domain simulation and frequency-domain analysis of the complete system. The model includes the full set of feedback loops and nonlinear elements such as the beam and klystron. The model may be used to predict beam and feedback stability in the presence of nonlinearities through time-domain simulation as well as system frequency response about a given operating point

Calibration of the beam-position monitor system for the SLAC PEP-II B factory

International Nuclear Information System (INIS)

Johnson, R.; Smith, S.; Kurita, N.

1997-06-01

The Beam-Position Monitors (BPM) for the PEP-II B Factory consist of four 1.5-cm diameter button style pickups mounted on the diagonals of the quadrupole vacuum chambers. Before installation of the vacuum chambers in the quadrupole assemblies, the electrical center of the BPMs is measured with respect to the mechanical center in a calibration test stand. In this paper the calibration test stand is described and the precision and accuracy of the calibrations are presented. After installation of the quadrupole assemblies in the PEP-II tunnel, the passive attenuation for each channel of the system is measured to preserve the accuracy of the calibration. Finally, the active electronics includes an onboard calibrator. Results for these portions of the calibration are presented

Rapid radiochemical procedures for a process support laboratory

International Nuclear Information System (INIS)

Beals, D.M.

1998-01-01

An on-site mobile laboratory has been installed near a groundwater treatment facility, and rapid analytical procedures have been deployed for determining sample activity in the process support laboratory. The required analyses to support the remediation project include gross alpha/, gross nonvolatile beta, 90 Sr, 99 Tc, 137 Cs and total Ra (226 + 228). The present mission of the Savannah River Site (SRS), a US Department of Energy nuclear production facility, is one of nuclear waste stabilization and of environmental restoration and remediation. Because of previous practices of disposing low-level radioactive waste to seepage basins, some of the groundwater under the SRS has become contaminated with radioactive species. A water treatment facility has been installed to remediate the groundwater below the old F and H areas' seepage basins. Groundwater is pumped from the contaminated aquifer through a series of filtration, ion exchange, and reverse osmosis steps and when cleaned is reinjected back into the aquifer. Samples are pulled from various points in the treatment facility to ensure that the process is working as designed. In order to minimize turnaround time for these analyses, a process control station (i.e., a mobile on-site laboratory) has been installed at the F area water treatment unit, and rapid radioanalytical procedures have been deployed

A Computerized Clinical Support System and Psychological Laboratory.

Science.gov (United States)

Cassel, Russell N.

1978-01-01

Advocating "holistic" medicine, this article details the benefits to be derived from using a computerized clinical support system in a psychological laboratory focusing on internal healing where the client/patient becomes a committed partner utilizing biofeedback equipment, gaming, and simulation to achieve self-understanding and…

Development of the Positive Emotions Program for Schizophrenia (PEPS: an intervention to improve pleasure and motivation in schizophrenia

Directory of Open Access Journals (Sweden)

Alexandra eNguyen

2016-02-01

Full Text Available Objectives: The efficacy of drug-based treatments and psychological interventions on the primary negative symptoms of schizophrenia remains limited. Recent literature has distinguished negative symptoms associated with a diminished capacity to experience, from those associated with a limited capacity for expression. The Positive Emotions Program for Schizophrenia (PEPS is a new method that specifically aims to reduce the syndrome of a diminished capacity to experience. Methods: The intervention’s vital ingredients were identified through a literature review of emotion in schizophrenia and positive psychology. The program has been beta-tested on various groups of healthcare professionals. Results: A detailed description of the final version of PEPS is presented here. The French version of the program is freely downloadable. Conclusions: PEPS is a specific, short, easy to use, group-based intervention to improve pleasure and motivation in schizophrenia. It was built considering a recovery-oriented approach to schizophrenia.

The PEP-II Asymmetric B Factory: Design details and R ampersand D results

International Nuclear Information System (INIS)

Bloom, E.; DeStaebler, H.; Dorfan, J.

1994-06-01

PEP-II, a 9 GeV x 3.1 GeV electron-positron collider with a design luminosity of 3 x 10 33 cm -2 s -1 has now been approved for construction by SLAC, LBL and LLNL for the purpose of studying CP violation in the B bar B system. This upgrade project involves replacing the vacuum and RF systeum of PEP, which will serve as the high-energy ring (HER), along with the addition of a new low-energy ring (LER) mounted atop the HER. Designs for both rings are described, and the anticipated project construction schedule is indicated. Collider operation will begin at the end of 1998. An aggressive R ampersand D program has been carried out to validate our design choices; key results in the areas of lattice design, vacuum, RF, and multibunch feedback are summarized

Trigger drift chamber for the upgraded mark II detector at PEP

Science.gov (United States)

Ford, W. T.; Smith, J. G.; Wagner, S. R.; Weber, P.; White, S. L.; Alvarez, M.; Calviño, F.; Fernandez, E.

1987-04-01

A small cylindrical track detector was built as an array of single-wire drift cells with aluminized mylar cathode tubes. Point measurement resolution of ˜ 90 μm was achieved with a drift gas of 50% argon-50% ethane at atmospheric pressure. The chamber construction, electronics, and calibration are discussed. Performance results from PEP colliding-beam data are presented.

Construction, characterization and evaluation of the protective efficacy of the Streptococcus suis double mutant strain ΔSsPep/ΔSsPspC as a live vaccine candidate in mice.

Science.gov (United States)

Hu, Jin; You, Wujin; Wang, Bin; Hu, Xueying; Tan, Chen; Liu, Jinlin; Chen, Huanchun; Bei, Weicheng

2015-01-01

Streptococcus suis serotype 2 (S. suis 2) causes sepsis and meningitis in piglets and humans, and results in one of the most serious bacterial diseases affecting the production of commercial pigs around the world. Due to the failure of the current inactivated vaccine to protect against the disease, development of a new attenuated live vaccine against S. suis 2 by deleting essential virulence factors is urgently needed. We have previously reported the construction and characterization of an SsPep single gene deletion mutant strain ΔSsPep based on S. suis 2. Our previous results have shown that SsPep plays a critical role in the pathogenesis of S. suis 2. In this study, a precisely defined double-deletion mutant ΔSsPep/ΔSsPspC of S. suis 2 without antibiotic-resistance markers was constructed based on ΔSsPep, and the levels of virulence of the wild-type (WT) and ΔSsPep/ΔSsPspC were compared in a mouse experimental infection model. We demonstrated that the double mutant ΔSsPep/ΔSsPspC was less virulent than the WT, and could induce a noticeable antibody response. Analysis of IgG subclasses (IgG1 and IgG2a) indicated that both Th1 and Th2 responses were induced by ΔSsPep/ΔSsPspC, although the IgG2a (Th1) response predominated over the IgG1 (Th2) response. Moreover, ΔSsPep/ΔSsPspC could confer 90% protective efficacy against challenge with a lethal dose of fully virulent S. suis 2. Taken together, these data demonstrate that ΔSsPep/ΔSsPspC can be used as an effective live vaccine and provide a novel strategy against infection of S. suis 2. Copyright © 2014 Elsevier GmbH. All rights reserved.

PEP725 Pan European Phenological Database

Science.gov (United States)

Koch, E.; Adler, S.; Lipa, W.; Ungersböck, M.; Zach-Hermann, S.

2010-09-01

Europe is in the fortunate situation that it has a long tradition in phenological networking: the history of collecting phenological data and using them in climatology has its starting point in 1751 when Carl von Linné outlined in his work Philosophia Botanica methods for compiling annual plant calendars of leaf opening, flowering, fruiting and leaf fall together with climatological observations "so as to show how areas differ". Recently in most European countries, phenological observations have been carried out routinely for more than 50 years by different governmental and non governmental organisations and following different observation guidelines, the data stored at different places in different formats. This has been really hampering pan European studies as one has to address many network operators to get access to the data before one can start to bring them in a uniform style. From 2004 to 2009 the COST-action 725 established a European wide data set of phenological observations. But the deliverables of this COST action was not only the common phenological database and common observation guidelines - COST725 helped to trigger a revival of some old networks and to establish new ones as for instance in Sweden. At the end of 2009 the COST action the database comprised about 8 million data in total from 15 European countries plus the data from the International Phenological Gardens IPG. In January 2010 PEP725 began its work as follow up project with funding from EUMETNET the network of European meteorological services and of ZAMG the Austrian national meteorological service. PEP725 not only will take over the part of maintaining, updating the COST725 database, but also to bring in phenological data from the time before 1951, developing better quality checking procedures and ensuring an open access to the database. An attractive webpage will make phenology and climate impacts on vegetation more visible in the public enabling a monitoring of vegetation development.

Status of the Object Oriented Program based Graphic User Interface Development for HyPEP

International Nuclear Information System (INIS)

Lee, Young Jin; Park, Ji Won; Hwang, Moon Kyu

2007-01-01

Hydrogen is being promoted as the energy-carrier for the future under the proposed hydrogen economy. As large reservoirs of hydrogen molecules do not exist in nature, the hydrogen needs to be produced using other energies such as nuclear, coal, natural gas, etc. The nuclear hydrogen production facility, where nuclear power is used to split water to produce hydrogen molecules, is presented as one of the optimal solutions for the large scale hydrogen production. Compared to other large scale hydrogen production methods, the nuclear hydrogen production can have better overall economics and less damaging environmental impacts such as global warming and pollution. In order to assess the overall economics of the hydrogen production, it is important to assess the hydrogen production efficiencies. In the conceptual design stage of the plant, various different plant layouts need to be studied to optimize the production efficiency. The main development aim of the HyPEP (Hydrogen Production Efficiency calculator Program) is the rapid assessment of the overall hydrogen production efficiency of a nuclear hydrogen production facility. The program is being developed with extensive GUI features to enhance the user-friendliness and the rapid model build-ups. This paper presents the current status of the development of HyPEP especially the GUI. HyPEP is being developed under an International Nuclear Engineering Research Initiative (I-NERI) Project

Epidermal growth factor inhibits glycylsarcosine transport and hPepT1 expression in a human intestinal cell line

DEFF Research Database (Denmark)

Nielsen, C U; Amstrup, J; Steffansen, B

2001-01-01

(max) decreased from 2.61 +/- 0.4 to 1.06 +/- 0.1 nmol x cm(-2) x min(-1) (n = 3, P PepT1 mRNA (using glucose-6-phosphate dehydrogenase mRNA as control......) in cells treated with EGF. Western blotting indicated a decrease in hPepT1 protein in cell lysates. We conclude that EGF treatment decreases Gly-Sar transport in Caco-2 cells by decreasing the number of peptide transporter molecules in the apical membrane....

Exopolysaccharide-associated protein sorting in environmental organisms: the PEP-CTERM/EpsH system. Application of a novel phylogenetic profiling heuristic

Directory of Open Access Journals (Sweden)

Ward Naomi

2006-08-01

Full Text Available Abstract Background Protein translocation to the proper cellular destination may be guided by various classes of sorting signals recognizable in the primary sequence. Detection in some genomes, but not others, may reveal sorting system components by comparison of the phylogenetic profile of the class of sorting signal to that of various protein families. Results We describe a short C-terminal homology domain, sporadically distributed in bacteria, with several key characteristics of protein sorting signals. The domain includes a near-invariant motif Pro-Glu-Pro (PEP. This possible recognition or processing site is followed by a predicted transmembrane helix and a cluster rich in basic amino acids. We designate this domain PEP-CTERM. It tends to occur multiple times in a genome if it occurs at all, with a median count of eight instances; Verrucomicrobium spinosum has sixty-five. PEP-CTERM-containing proteins generally contain an N-terminal signal peptide and exhibit high diversity and little homology to known proteins. All bacteria with PEP-CTERM have both an outer membrane and exopolysaccharide (EPS production genes. By a simple heuristic for screening phylogenetic profiles in the absence of pre-formed protein families, we discovered that a homolog of the membrane protein EpsH (exopolysaccharide locus protein H occurs in a species when PEP-CTERM domains are found. The EpsH family contains invariant residues consistent with a transpeptidase function. Most PEP-CTERM proteins are encoded by single-gene operons preceded by large intergenic regions. In the Proteobacteria, most of these upstream regions share a DNA sequence, a probable cis-regulatory site that contains a sigma-54 binding motif. The phylogenetic profile for this DNA sequence exactly matches that of three proteins: a sigma-54-interacting response regulator (PrsR, a transmembrane histidine kinase (PrsK, and a TPR protein (PrsT. Conclusion These findings are consistent with the hypothesis

Operation and performance of the PEP-II prototype longitudinal damping system at ALS

International Nuclear Information System (INIS)

Teytelman, D.; Claus, R.; Fox, J.

1995-05-01

A modular programmable longitudinal feedback system has been developed as a component of the PEP-II R+D program. This system is based on a family of VME and VXI packaged signal processing functions which implement a general purpose digital feedback controller for accelerators with bunch spacings of 2 ns. A complete PEP-II prototype system has been configured and installed for use at the LBL Advanced Light Source. The system configuration used for tests at the ALS is described and results are presented showing the action of the feedback system. Open and closed loop results showing the detection and calculation of feedback signals from bunch motion are presented and the system is shown to damp coupled-bunch instabilities in the ALS. Use of the system for accelerator diagnostics is illustrated via measurement of grow-damp transients which quantify growth rates without feedback, damping rates with feedback, and identify unstable modes

Trigger drift chamber for the upgraded Mark II detector at PEP

International Nuclear Information System (INIS)

Ford, W.T.; Smith, J.G.; Wagner, S.R.; Weber, P.; White, S.L.; Alvarez, M.; Calvino, F.; Fernandez, E.; Universidad Autonoma de Barcelona

1987-01-01

A small cylindrical track detector was built as an array of single-wire drift cells with aluminized mylar cathode tubes. Point measurement resolution of ∝90 μm was achieved with a drift gas of 50% argon-50% ethane at atmospheric pressure. The chamber construction, electronics, and calibration are discussed. Performance results from PEP colliding-beam data are presented. (orig.)

Positive educative programme : A whole school approach to supporting children’s well-being and creating a positive school climate: a pilot study

NARCIS (Netherlands)

Elfrink, Teuntje R.; Goldberg, Jochem M.; Schreurs, Karlein M.G.; Bohlmeijer, Ernst T.; Clarke, Aleisha M.

2017-01-01

Purpose: The purpose of this paper is to report on a process and impact evaluation of the Positief Educatief Programma (Positive Education Programme (PEP)), a whole school approach to supporting children’s well-being and creating a positive school climate in primary schools in the Netherlands. PEP

Positive educative programme : A whole school approach to supporting children’s well-being and creating a positive school climate: a pilot study

NARCIS (Netherlands)

Elfrink, Teuntje R.; Goldberg, Jochem M.; Schreurs, Karlein M.G.; Bohlmeijer, Ernst T.; Clarke, Aleisha M.

2017-01-01

Purpose The purpose of this paper is to report on a process and impact evaluation of the Positief Educatief Programma (Positive Education Programme (PEP)), a whole school approach to supporting children’s well-being and creating a positive school climate in primary schools in the Netherlands. PEP

Supply chain management of laboratory supportive services and its ...

African Journals Online (AJOL)

Supply chain management of laboratory supportive services and its potential implications on the quality of HIV diagnostic services in Tanzania. ... Results: A total of 39 health facilities (HF) were included in the study. This included 23 public ...

PEP-II Transverse Feedback Electronics Upgrade

International Nuclear Information System (INIS)

Weber, J.; Chin, M.; Doolittle, L.; Akre, R.

2005-01-01

The PEP-II B Factory at the Stanford Linear Accelerator Center (SLAC) requires an upgrade of the transverse feedback system electronics. The new electronics require 12-bit resolution and a minimum sampling rate of 238 Msps. A Field Programmable Gate Array (FPGA) is used to implement the feedback algorithm. The FPGA also contains an embedded PowerPC 405 (PPC-405) processor to run control system interface software for data retrieval, diagnostics, and system monitoring. The design of this system is based on the Xilinx(R) ML300 Development Platform, a circuit board set containing an FPGA with an embedded processor, a large memory bank, and other peripherals. This paper discusses the design of a digital feedback system based on an FPGA with an embedded processor. Discussion will include specifications, component selection, and integration with the ML300 design

PEP-II Transverse Feedback Electronics Upgrade

International Nuclear Information System (INIS)

Weber, J.M.; Chin, M.J.; Doolittle, L.R.; Akre, R.

2006-01-01

The PEP-II B Factory at the Stanford Linear Accelerator Center (SLAC) requires an upgrade of the transverse feedback system electronics. The new electronics require 12-bit resolution and a minimum sampling rate of 238 Msps. A Field Programmable Gate Array (FPGA) is used to implement the feedback algorithm. The FPGA also contains an embedded PowerPC 405 (PPC-405) processor to run control system interface software for data retrieval, diagnostics, and system monitoring. The design of this system is based on the Xilinx(reg s ign) ML300 Development Platform, a circuit board set containing an FPGA with an embedded processor, a large memory bank, and other peripherals. This paper discusses the design of a digital feedback system based on an FPGA with an embedded processor. Discussion will include specifications, component selection, and integration with the ML300 design

PEP-II Transverse Feedback Electronics Upgrade

CERN Document Server

Weber, Jonah; Chin, Michael; Doolittle, Lawrence

2005-01-01

The PEP-II B Factory at the Stanford Linear Accelerator Center (SLAC) requires an upgrade of the transverse feedback system electronics. The new electronics require 12-bit resolution and a minimum sampling rate of 238 Msps. A Field Programmable Gate Array (FPGA) is used to implement the feedback algorithm. The FPGA also contains an embedded PowerPC 405 (PPC-405) processor to run control system interface software for data retrieval, diagnostics, and system monitoring. The design of this system is based on the Xilinx® ML300 Development Platform, a circuit board set containing an FPGA with an embedded processor, a large memory bank, and other peripherals. This paper discusses the design of a digital feedback system based on an FPGA with an embedded processor. Discussion will include specifications, component selection, and integration with the ML300 design.

Search for Ne in e−μ+ events at petra and pep energies

Directory of Open Access Journals (Sweden)

K. Yamanashi

1978-03-01

Full Text Available We propose a way to check the possible existence of an electron-type neutral heavy lepton Ne which decays into e−μ+νμ. In the region of cosθcoll < 0 eμ signals due to Ne are expected to exceed those due to τ± at PETRA and PEP energies.

Laboratory support during and after the Ebola virus endgame: Towards a sustained laboratory infrastructure

NARCIS (Netherlands)

I. Goodfellow; C.B.E.M. Reusken (Chantal); M.P.G. Koopmans D.V.M. (Marion)

2015-01-01

textabstractThe Ebola virus epidemic in West Africa is on the brink of entering a second phase in which the (inter)national efforts to slow down virus transmission will be engaged to end the epidemic. The response community must consider the longevity of their current laboratory support, as it is

PEP-X: An Ultimate Storage Ring Based on Fourth-Order Geometric Achromats

Energy Technology Data Exchange (ETDEWEB)

Cai, Yunhai; Bane, Karl; Hettel, Robert; Nosochkov, Yuri; Wang, Min-Huey; /SLAC

2012-04-06

We have designed an 'ultimate' storage ring for the PEP-X light source that achieves the diffraction limited emittances (at 1.5 {angstrom}) of 12 pm-rad in both horizontal and vertical planes with a 4.5-GeV beam. These emittances include the contribution of intrabeam scattering at a nominal current of 200 mA in 3300 bunches. This quality beam in conjunction with a conventional 4-m undulator in a straight section can generate synchrotron radiation having a spectral brightness above 10{sup 22} [photons/s/mm{sup 2}/mrad{sup 2}/0.1% BW] at a 10 keV photon energy. The high coherence at the diffraction limit makes PEP-X competitive with 4th generation light sources based on an energy recovery linac. In addition, the beam lifetime is several hours and the dynamic aperture is large enough to allow off-axis injection. The alignment and stability tolerances, though challenging, are achievable. A ring with all these properties is only possible because of several major advances in mitigating the effects of nonlinear resonances.

RF Feedback Analysis for 4 cavities per klystron in PEP-II

International Nuclear Information System (INIS)

Corredoura, P.; Tighe, R.

1994-06-01

Lattice changes in the PEP-II high energy ring have made the concept of driving four cavities with a single klystron an attractive option. This paper examines the topology from a RF feedback point of view. Sources of error are identified and their magnitudes are estimated. The effect on the performance of the longitudinal impedance reducing feedback loops is calculated using control theory and Mathematica

In Depth Diagnostics for RF System Operation in the PEP-II B Factory

International Nuclear Information System (INIS)

Van Winkle, Daniel; Fox, John; Teytelman, Dmitry; SLAC

2005-01-01

The PEP-II RF systems incorporate numerous feedback loops in the low-level processing for impedance control and operating point regulation. The interaction of the multiple loops with the beam is complicated, and the systems incorporate online diagnostic tools to configure the feedback loops as well as to record fault files in the case of an RF abort. Rapid and consistent analysis of the RF-related beam aborts and other failures is critical to the reliable operation of the B-Factory, especially at the recently achieved high beam currents. Procedures and algorithms used to extract diagnostic information from time domain fault files are presented and illustrated via example interpretations of PEP-II fault file data. Example faults presented will highlight the subtle interpretation required to determine the root cause. Some such examples are: abort kicker firing asynchronously, klystron and cavity arcs, beam loss leading to longitudinal instability, tuner read back jumps and poorly configured low-level RF feedback loop

TRACKING SIMULATIONS NEAR HALF-INTEGER RESONANCE AT PEP-II

International Nuclear Information System (INIS)

Nosochkov, Yuri

2003-01-01

Beam-beam simulations predict that PEP-II luminosity can be increased by operating the horizontal betatron tune near and above a half-integer resonance. However, effects of the resonance and its synchrotron sidebands significantly enhance betatron and chromatic perturbations which tend to reduce dynamic aperture. In the study, chromatic variation of horizontal tune near the resonance was minimized by optimizing local sextupoles in the Interaction Region. Dynamic aperture was calculated using tracking simulations in LEGO code. Dependence of dynamic aperture on the residual orbit, dispersion and β distortion after correction was investigated

Analysis of the wake field effects in the PEP-II storage rings with extremely high currents

Energy Technology Data Exchange (ETDEWEB)

Novokhatski, A., E-mail: novo@slac.stanford.edu; Seeman, J.; Sullivan, M.

2014-01-21

We present the history and analysis of different wake field effects throughout the operational life of the PEP-II SLAC B-factory. Although the impedance of the high and low energy rings is small, the intense high-current beams generated a lot of power. The effects from these wake fields are: heating and damage of vacuum beam chamber elements like RF seals, vacuum valves, shielded bellows, BPM buttons and ceramic tiles; vacuum spikes, vacuum instabilities and high detector background; and beam longitudinal and transverse instabilities. We also discuss the methods used to eliminate these effects. Results of this analysis and the PEP-II experience may be very useful in the design of new storage rings and light sources.

Modeling Lost-Particle Accelerator Backgrounds in PEP-II Using LPTURTLE

CERN Document Server

Fieguth, Theodore; Kozanecki, Witold

2005-01-01

Background studies during the design, construction, commissioning, operation and improvement of BaBar and PEP-II have been greatly influenced by results from a program referred to as LPTURTLE (Lost Particle TURTLE a modified version of Decay TURTLE) which was originally conceived for the purpose of studying gas background for SLC. This venerable program is still in use today. We describe its use, capabilities and improvements and refer to current results now being applied to BaBar.

Promoting Good Clinical Laboratory Practices and Laboratory Accreditation to Support Clinical Trials in Sub-Saharan Africa

Science.gov (United States)

Shott, Joseph P.; Saye, Renion; Diakité, Moussa L.; Sanogo, Sintry; Dembele, Moussa B.; Keita, Sekouba; Nagel, Mary C.; Ellis, Ruth D.; Aebig, Joan A.; Diallo, Dapa A.; Doumbo, Ogobara K.

2012-01-01

Laboratory capacity in the developing world frequently lacks quality management systems (QMS) such as good clinical laboratory practices, proper safety precautions, and adequate facilities; impacting the ability to conduct biomedical research where it is needed most. As the regulatory climate changes globally, higher quality laboratory support is needed to protect study volunteers and to accurately assess biological parameters. The University of Bamako and its partners have undertaken a comprehensive QMS plan to improve quality and productivity using the Clinical and Laboratory Standards Institute standards and guidelines. The clinical laboratory passed the College of American Pathologists inspection in April 2010, and received full accreditation in June 2010. Our efforts to implement high-quality standards have been valuable for evaluating safety and immunogenicity of malaria vaccine candidates in Mali. Other disease-specific research groups in resource-limited settings may benefit by incorporating similar training initiatives, QMS methods, and continual improvement practices to ensure best practices. PMID:22492138

Impedance analysis of the PEP-II vacuum chamber

International Nuclear Information System (INIS)

Ng, C.K.; Weiland, T.

1995-05-01

The PEP-II high energy ring (HER) vacuum chamber consists of a copper tube with periodically spaced pumping slots. The impedance of the vacuum chamber due to the slots is analyzed. Both narrow-band and broadband impedances are considered as well as longitudinal and transverse components thereof. It is found that although the broad-band impedance is tolerable, the narrow-band impedance may exceed the instability limit given by the natural damping with no feedback system on. Traveling wave modes in the chamber are the major source of this high value narrow-band impedance. We also study the dependences of the impedance on the slot length and the geometrical cross section

Design of a Clinical Information Management System to Support DNA Analysis Laboratory Operation

Science.gov (United States)

Dubay, Christopher J.; Zimmerman, David; Popovich, Bradley

1995-01-01

The LabDirector system has been developed at the Oregon Health Sciences University to support the operation of our clinical DNA analysis laboratory. Through an iterative design process which has spanned two years, we have produced a system that is both highly tailored to a clinical genetics production laboratory and flexible in its implementation, to support the rapid growth and change of protocols and methodologies in use in the field. The administrative aspects of the system are integrated with an enterprise schedule management system. The laboratory side of the system is driven by a protocol modeling and execution system. The close integration between these two aspects of the clinical laboratory facilitates smooth operations, and allows management to accurately measure costs and performance. The entire application has been designed and documented to provide utility to a wide range of clinical laboratory environments.

Responses of mRNA expression of PepT1 in small intestine to ...

African Journals Online (AJOL)

To study the effect of circulation small peptides concentration on mRNA expression in small intestine, graded amount of soybean small peptides (SSP) were infused into lactating goats through duodenal fistulas. Peptide-bound amino acid (PBAA) concentration in arterial plasma and the mRNA expression of PepT1 was ...

Design of an electronic performance support system for food chemistry laboratory classes

NARCIS (Netherlands)

Kolk, van der J.

2013-01-01

The design oriented research described in this thesis aims at designing an realizing an electronic performance support system for food chemistry laboratory classes (labEPSS). Four design goals related to food chemistry laboratory classes were identified. Firstly, labEPSS should avoid extraneous

PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress

Science.gov (United States)

Ding, Jun; Holzwarth, Garrett; Bradford, C. Samuel; Cooley, Ben; Yoshinaga, Allen S.; Patton-Vogt, Jana; Abeliovich, Hagai; Penner, Michael H.; Bakalinsky, Alan T.

2017-01-01

In fungi, two recognized mechanisms contribute to pH homeostasis: the plasma membrane proton-pumping ATPase that exports excess protons and the vacuolar proton-pumping ATPase (V-ATPase) that mediates vacuolar proton uptake. Here, we report that overexpression of PEP3 which encodes a component of the HOPS and CORVET complexes involved in vacuolar biogenesis, shortened lag phase in Saccharomyces cerevisiae exposed to acetic acid stress. By confocal microscopy, PEP3-overexpressing cells stained with the vacuolar membrane-specific dye, FM4-64 had more fragmented vacuoles than the wild-type control. The stained overexpression mutant was also found to exhibit about 3.6-fold more FM4-64 fluorescence than the wild-type control as determined by flow cytometry. While the vacuolar pH of the wild-type strain grown in the presence of 80 mM acetic acid was significantly higher than in the absence of added acid, no significant difference was observed in vacuolar pH of the overexpression strain grown either in the presence or absence of 80 mM acetic acid. Based on an indirect growth assay, the PEP3-overexpression strain exhibited higher V-ATPase activity. We hypothesize that PEP3 overexpression provides protection from acid stress by increasing vacuolar surface area and V-ATPase activity and, hence, proton-sequestering capacity. PMID:26051671

A unique power supply for the PEP II klystron at SLAC

International Nuclear Information System (INIS)

Cassel, R.; Nguyen, M.N.

1997-07-01

Each of the eight 1.2 MW RF klystrons for the PEP-II storage rings require a 2.5 MVA DC power supply of 83 Kv at 23 amps. The design for the supply was base on three factors, low cost, small size to fit existing substation pads, and good protection against damage to the klystron including klystron gun arcs. The supply uses a 12 pulse 12.5 KV primary thyristor star point controller with primary filter inductor to provide rapid voltage control, good voltage regulation, and fast turn off during klystron tube faults. The supply also uses a unique secondary rectifier, filter capacitor configuration to minimize the energy available under a klystron fault. The voltage control is from 0--90 KV with a regulation of < 0.1% and voltage ripple of < 1% P-P, (< 0.2% RMS) above 60 KV. The supply utilizes a thyristor crowbar, which under a klystron tube arc limits the energy in the klystron arc to < 5 joules. If the thyristor crowbar is disabled the energy supplied is < 40 joules into the arc. The size of the supply was reduced small enough to fit the existing PEP transformer yard pads. The cost of the power supply was < $140 per KVA

PEP-II RF Cavity Revisited (LCC-0032)

Energy Technology Data Exchange (ETDEWEB)

Rimmer, R.

2004-03-23

This report describes the results of numerical simulations of the PEP-II RF cavity performed after the completion of the construction phase of the project and comparisons are made to previous calculations and measured results. These analyses were performed to evaluate new calculation techniques for the HOM distribution and RF surface heating that were not available at the time of the original design. These include the use of a high frequency electromagnetic element in ANSYS and the new Omega 3P code to study wall losses, and the development of broadband time domain simulation methods in MAFIA for the HOM loading. The computed HOM spectrum is compared with cavity measurements and observed beam-induced signals. The cavity fabrication method is reviewed, with the benefit of hindsight, and simplifications are discussed.

Functionalized PLA-PEG nanoparticles targeting intestinal transporter PepT1 for oral delivery of acyclovir.

Science.gov (United States)

Gourdon, Betty; Chemin, Caroline; Moreau, Amélie; Arnauld, Thomas; Baumy, Philippe; Cisternino, Salvatore; Péan, Jean-Manuel; Declèves, Xavier

2017-08-30

Targeting intestinal di- and tri-peptide transporter PepT1 with prodrugs is a successful strategy to improve oral drug bioavailability, as demonstrated with valacyclovir, a prodrug of acyclovir. The aim of this new drug delivery strategy is to over-concentrate a poorly absorbed drug on the intestinal membrane surface by targeting PepT1 with functionalized polymer nanoparticles. In the present study, poly(lactic acid)-poly(ethylene glycol)-ligand (PLA-PEG-ligand) nanoparticles were obtained by nanoprecipitation. A factorial experimental design allowed us to identify size-influent parameters and to obtain optimized ≈30nm nanoparticles. Valine, Glycylsarcosine, Valine-Glycine, and Tyrosine-Valine were chemically linked to PLA-PEG. In Caco-2 cell monolayer model, competition between functionalized nanoparticles and [ 3 H]Glycylsarcosine, a strong substrate of PepT1, reduced [ 3 H]Glycylsarcosine transport from 22 to 46%. Acyclovir was encapsulated with a drug load of ≈10% in valine-functionalized nanoparticles, resulting in a 2.7-fold increase in permeability as compared to the free drug. An in vivo pharmacokinetic study in mice compared oral absorption of acyclovir after administration of 25mg/kg of valacyclovir, free or encapsulated acyclovir in functionalized nanoparticles. Acyclovir encapsulation did not statistically modify AUC or C max , but increased t 1/2 and MRT 1.3-fold as compared to free acyclovir. This new strategy is promising for poorly absorbed drugs by oral administration. Copyright © 2017 Elsevier B.V. All rights reserved.

RF feedback development for the PEP-II B Factory

Energy Technology Data Exchange (ETDEWEB)

Corredoura, P.; Sapozhnikov, L.; Tighe, R.

1994-06-01

In PEP-II heavy beam loading along with a relatively long revolution period combine to strongly drive lower coupled-bunch modes through interaction with the fundamental cavity mode. Feedback techniques can be applied to reduce the cavity impedance seen by the beam. Several RF feedback loops are planned to reduce the growth rates down to a level which can be damped by the relatively low power bunch-by-bunch longitudinal feedback system. This paper describes the RF feedback loops as well as hardware tests using a 500 kW klystron, analog and digital feedback loops, and a low power test cavity.

RF feedback development for the PEP-II B Factory

International Nuclear Information System (INIS)

Corredoura, P.; Sapozhnikov, L.; Tighe, R.

1994-06-01

In PEP-II heavy beam loading along with a relatively long revolution period combine to strongly drive lower coupled-bunch modes through interaction with the fundamental cavity mode. Feedback techniques can be applied to reduce the cavity impedance seen by the beam. Several RF feedback loops are planned to reduce the growth rates down to a level which can be damped by the relatively low power bunch-by-bunch longitudinal feedback system. This paper describes the RF feedback loops as well as hardware tests using a 500 kW klystron, analog and digital feedback loops, and a low power test cavity

Study of heavy quark production with the Mark II at PEP

International Nuclear Information System (INIS)

Abrams, G.; Amidei, D.; Baden, A.

1983-10-01

The methods adopted by the Mark II collaboration to study heavy quark production at PEP are described. Two complementary techniques are used: D* tagging using the decay chain D* + . D 0 π + , D 0 → K - π + , and inclusive lepton tagging using the characteristic p/sub T/ distributions to distinguish contributions from b and c quarks. These techniques are used to derive information about heavy quark fragmentation and about the weak coupling of heavy quarks

Study of an instability of the PEP-II positron beam (Ohmi effect and Multipactoring)

Energy Technology Data Exchange (ETDEWEB)

Heifets, S A [Stanford Linear Accelerator Center, Menlo Park, CA (United States)

1996-08-01

The processes defining the density distribution of the photoelectrons are quite complicated. In this study, a simplified model of the instability was used to get a quick estimate of the growth rate of the instability and the relative importance of the parameters, as has been done in Ohmi`s paper. The production rate and dynamics of the photoelectrons are studied for the PEP-II parameters. The growth rate of the transverse instability driven by the primary photoelectrons is of the order of 0.7 msec for the PEP-II parameters. The multipactoring at resonance currents cannot produce large electron density due to the final energy spread caused by the finite bunch length and the intrinsic energy spread of the secondary electrons. Production of the secondary electrons may lead to large average densities. The ion can be produced in electron collisions with the residual gas with density of the order of the electron density. (G.K.)

Search for scalar electrons at PEP

International Nuclear Information System (INIS)

Wilson, R.J.

1983-08-01

Experimental results from e + e - reactions at the Positron Electron Project (PEP) using the High Resolution Spectrometer (HRS) are presented. Events with two electrons, and no other charged particles, in the final state are studied. Limits are given for the production of scalar-electrons predicted by models based on supersymmetry. In particular the pair production of such particles through s-channel single photon annihilation and t-channel inelastic scattering is considered. The data are well described by quantum electrodynamics (QED) but we observe one event which is also consistent with a supersymmetric model. Using this single event we find that the mass, M/sub se/, of these scalar-electrons es excluded, to 95% CL, in the range 1.8 less than or equal to M/sub se/ less than or equal to 14.2 GeV/c 2 . A description of the HRS detector is given with particular emphasis on the electronic trigger system

Universal immunogenicity validation and assessment during early biotherapeutic development to support a green laboratory.

Science.gov (United States)

Bautista, Ami C; Zhou, Lei; Jawa, Vibha

2013-10-01

Immunogenicity support during nonclinical biotherapeutic development can be resource intensive if supported by conventional methodologies. A universal indirect species-specific immunoassay can eliminate the need for biotherapeutic-specific anti-drug antibody immunoassays without compromising quality. By implementing the R's of sustainability (reduce, reuse, rethink), conservation of resources and greener laboratory practices were achieved in this study. Statistical analysis across four biotherapeutics supported identification of consistent product performance standards (cut points, sensitivity and reference limits) and a streamlined universal anti-drug antibody immunoassay method implementation strategy. We propose an efficient, fit-for-purpose, scientifically and statistically supported nonclinical immunogenicity assessment strategy. Utilization of a universal method and streamlined validation, while retaining comparability to conventional immunoassays and meeting the industry recommended standards, provides environmental credits in the scientific laboratory. Collectively, individual reductions in critical material consumption, energy usage, waste and non-environment friendly consumables, such as plastic and paper, support a greener laboratory environment.

On-site laboratory support of Oak Ridge National Laboratory environmental restoration field activities

International Nuclear Information System (INIS)

Burn, J.L.E.

1995-07-01

A remedial investigation/feasibility study has been undertaken at Oak Ridge National Laboratory (ORNL). Bechtel National, Inc. and partners CH2M Hill, Ogden Environmental and Energy Services, and PEER Consultants are contracted to Lockheed Martin Energy Systems, performing this work for ORNL's Environmental Restoration (ER) Program. An on-site Close Support Laboratory (CSL) established at the ER Field Operations Facility has evolved into a laboratory where quality analytical screening results can be provided rapidly (e.g., within 24 hours of sampling). CSL capabilities include three basic areas: radiochemistry, chromatography, and wet chemistry. Radiochemical analyses include gamma spectroscopy, tritium and carbon-14 screens using liquid scintillation analysis, and gross alpha and beta counting. Cerenkov counting and crown-ether-based separation are the two rapid methods used for radiostrontium determination in water samples. By extending count times where appropriate, method detection limits can match those achieved by off-site contract laboratories. Volatile organic compounds are detected by means of gas chromatography using either headspace or purge and trap sample introduction (based on EPA 601/602). Ionic content of water samples is determined using ion chromatography and alkalinity measurement. Ion chromatography is used to quantify both anions (based on EPA 300) and cations. Wet chemistry procedures performed at the CSL include alkalinity, pH (water and soil), soil resistivity, and dissolved/suspended solids. Besides environmental samples, the CSL routinely screens health and safety and waste management samples. The cost savings of the CSL are both direct and indirect

EFRT M-12 Issue Resolution: Solids Washing

Energy Technology Data Exchange (ETDEWEB)

Baldwin, David L.; Schonewill, Philip P.; Toth, James J.; Huckaby, James L.; Eslinger, Paul W.; Hanson, Brady D.; Kurath, Dean E.; Minette, Michael J.

2010-01-01

Pacific Northwest National Laboratory (PNNL) was tasked by Bechtel National Inc. (BNI) on the River Protection Project-Hanford Tank Waste Treatment and Immobilization Plant (RPP-WTP) project to perform research and development activities to resolve technical issues identified for the Pretreatment Facility (PTF). The Pretreatment Engineering Platform (PEP) was designed, constructed, and operated as part of a plan to respond to issue M12, “Undemonstrated Leaching Processes” of the External Flowsheet Review Team (EFRT) issue response plan.( ) The PEP is a 1/4.5-scale test platform designed to simulate the WTP pretreatment caustic leaching, oxidative leaching, ultrafiltration solids concentration, and slurry washing processes. The PEP replicates the WTP leaching processes using prototypic equipment and control strategies. The PEP also includes non-prototypic ancillary equipment to support the core processing.

Dendritic cells induce specific cytotoxic T lymphocytes against prostate cancer TRAMP-C2 cells loaded with freeze- thaw antigen and PEP-3 peptide.

Science.gov (United States)

Liu, Xiao-Qi; Jiang, Rong; Li, Si-Qi; Wang, Jing; Yi, Fa-Ping

2015-01-01

Prostate cancer is the most common cancer in men. In this study, we investigated immune responses of cytotoxic T lymphocytes (CTLs) against TRAMP-C2 prostate cancer cells after activation by dendritic cells (DCs) loaded with TRAMP-C2 freeze-thaw antigen and/or PEP-3 peptide in vitro. Bone marrow-derived DC from the bone marrow of the C57BL/6 were induced to mature by using the cytokine of rhGM-CSF and rhIL-4, and loaded with either the freeze-thaw antigen or PEP-3 peptide or both of them. Maturation of DCs was detected by flow cytometry. The killing efficiency of the CTLs on TRAMP-C2 cells were detected by flow cytometry, CCK8, colony formation, transwell migration, and wound-healing assay. The levels of the IFN-γ, TNF-β and IL-12 were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the unloaded DCs, the loaded DCs had significantly increased expression of several phenotypes related to DC maturation. CTLs activated by DCs loaded with freeze-thaw antigen and PEP-3 peptide had more evident cytotoxicity against TRAMP-C2 cells in vitro. The secretion levels of IFN-γ, TNF-β and IL-12, secreted by DCs loaded with antigen and PEP-3 and interaction with T cells, were higher than in the other groups. Our results suggest that the CTLs activated by DCs loaded with TRAMP-C2 freeze-thaw antigen and PEP-3 peptide exert a remarkable killing efficiency against TRAMP-C2 cells in vitro.

Exploring the Potential of Smartphones and Tablets for Performance Support in Food Chemistry Laboratory Classes

NARCIS (Netherlands)

Kolk, van der J.; Hartog, R.; Gruppen, H.

2013-01-01

Increasingly, mobile applications appear on the market that can support students in chemistry laboratory classes. In a multiple app-supported laboratory, each of these applications covers one use-case. In practice, this leads to situations in which information is scattered over different screens and

A Project-Based Laboratory for Learning Embedded System Design with Industry Support

Science.gov (United States)

Lee, Chyi-Shyong; Su, Juing-Huei; Lin, Kuo-En; Chang, Jia-Hao; Lin, Gu-Hong

2010-01-01

A project-based laboratory for learning embedded system design with support from industry is presented in this paper. The aim of this laboratory is to motivate students to learn the building blocks of embedded systems and practical control algorithms by constructing a line-following robot using the quadratic interpolation technique to predict the…

Design of a Clinical Information Management System to Support DNA Analysis Laboratory Operation

OpenAIRE

Dubay, Christopher J.; Zimmerman, David; Popovich, Bradley

1995-01-01

The LabDirector system has been developed at the Oregon Health Sciences University to support the operation of our clinical DNA analysis laboratory. Through an iterative design process which has spanned two years, we have produced a system that is both highly tailored to a clinical genetics production laboratory and flexible in its implementation, to support the rapid growth and change of protocols and methodologies in use in the field. The administrative aspects of the system are integrated ...

Scaling of gain with energy spread and energy in the PEP FEL

International Nuclear Information System (INIS)

Fisher, A.S.

1992-01-01

The Sag Harbor paper on the PEP FEL discusses the scaling of various FEL parameters with energy spread σ var-epsilon . I will repeat some of this material here and then examine the benefit of increasing the energy spread. How much energy spread can be achieved with damping wigglers is the next topic. Finally, I consider the dependence of gain and saturation length on beam energy and undulator field

The key determinants of perceived external prestige (PEP) – Qualitative research approach

OpenAIRE

Tamara Sušanj Šulentić; Krešimir Žnidar; Jurica Pavičić

2017-01-01

Perceived external prestige (PEP) is a well-known concept oriented towards describing the way members of a certain organization interpret and assess their organisational reputation. Such perception can significantly affect employees’ identification and loyalty to the organisation as well as their job satisfaction and work performance. According to the social identification theory, people define themselves and others with respect to their belonging to a particular group or organization, and th...

Beam Aborts in PEP-II Rings and Lingering Drift Chamber Currents

International Nuclear Information System (INIS)

Meshkat, N.

2004-01-01

The BABAR detector at SLAC was designed to study CP-violation in B-meson decays from electron-positron collisions in the PEP-II electron-positron storage rings. Background radiation in the High Energy Ring (HER) and Low Energy Ring (LER) of PEP-II has the potential to damage the sensitive equipment in the BABAR detector. As a result, the beams in the HER and LER can be aborted to prevent such damage. In the span of a few microseconds, the HER and LER currents drop from, for example, 1450 micro Amps and 2300 micro Amps, respectively, to zero. At this time the voltage in the Drift Chamber is rapidly ramped down from a potential of 1930 V to a safe potential of 800 V, thus we would expect the currents in the Drift Chamber to quickly go to zero once the beams are aborted. However, we observe an average 15 second delay in the measured time it takes for all current in the Drift Chamber to fall below 1 micro Amp. This delay has been hypothesized as an instrumentation issue and not as a physical phenomenon. The specific sources of this error are still not completely known, but analysis suggests that it results from the interplay of the CAEN High Voltage supplies and the EPICS system and/or limitations within those systems

Exploring the Potential of Smartphones and Tablets for Performance Support in Food Chemistry Laboratory Classes

Science.gov (United States)

van der Kolk, Koos; Hartog, Rob; Beldman, Gerrit; Gruppen, Harry

2013-12-01

Increasingly, mobile applications appear on the market that can support students in chemistry laboratory classes. In a multiple app-supported laboratory, each of these applications covers one use-case. In practice, this leads to situations in which information is scattered over different screens and written materials. Such a multiple app-supported laboratory will become awkward with the growth of the number of applications and use cases. In particular, using and switching between applications is likely to induce extraneous cognitive load that can easily be avoided. The manuscript describes the design of a prototype smartphone web app (LabBuddy) designed to support students in food chemistry laboratory classes. The manuscript describes a case study ( n = 26) of the use of a LabBuddy prototype in such a laboratory class. Based on the evaluation of this case study, design requirements for LabBuddy were articulated. LabBuddy should work on HTML5 capable devices, independent of screen size, by having a responsive layout. In addition, LabBuddy should enable a student using LabBuddy to switch between devices without much effort. Finally, LabBuddy should offer an integrated representation of information.

Bunch-by-bunch longitudinal feedback system for PEP-II

International Nuclear Information System (INIS)

Oxoby, G.; Claus, R.; Fox, J.

1994-06-01

This paper describes the implementation of the bunch-by-bunch longitudinal feedback system for the PEP-II B Factory. Bunch spacing down to 2 ns is achieved using 500 Megasamples per second A/D and D/A converters, and AT ampersand T 1610 Digital Signal Processors are integrated to run a downsampled feedback algorithm for each bunch in parallel. This general purpose programmable system, packaged in VXI and VME, is modular and scalable to offer portability to other accelerator rings. The control and monitoring hardware and software architecture have been developed to provide ease of operation as well as diagnostic tools for machine physics

Numerical simulation of the PEP-II beam position monitor

Energy Technology Data Exchange (ETDEWEB)

Kurita, N; Martin, D; Ng, C -K; Smith, S [Stanford Linear Accelerator Center, Menlo Park, CA (United States); Weiland, T

1996-08-01

We use MAFIA to analyze the PEP-II button-type beam position monitor (BPM). Employing proper termination of the BPM into a coaxial cable, the output signal at the BPM is determined. Thus the issues of signal sensitivity and power output can be addressed quantitatively, including all transient effects and wakefields. Besides this first quantitative analysis of a true BPM 3D structure, we find that internal resonant modes are a major source of high value narrow-band impedances. The effects of these resonances on coupled-bunch instabilities are discussed. An estimate of the power dissipation in the ceramic vacuum seal under high current operation is given. (author)

Beam-beam effects for the PEP-II B Factory

International Nuclear Information System (INIS)

Furman, M.A.; Eden, J.R.

1993-05-01

We present a summary of a fairly extensive study of beam-beam issues that arise in the design of PEP-II. Most of these studies are carried out with ''strong-strong'' multiparticle tracking simulations. We focus on: choice of nominal beam-beam parameter, strength of the parasitic collisions, injection issues, performance and tradeoffs with unequal beam-beam parameters. We comment only briefly on beam lifetime. We conclude that: the beams are sufficiently well separated so that the parasitic collisions are effectively weak; that small inequalities in the beam-beam parameters may imply more comfortable performance margins; and that vertical injection with vertical beam separation is more comfortable than horizontal

Numerical simulation of the PEP-II beam position monitor

International Nuclear Information System (INIS)

Kurita, N.; Martin, D.; Ng, C.K.; Smith, S.; Weiland, T.

1995-09-01

The authors use MAFIA to analyze the PEP-II button-type beam position monitor (BPM). Employing proper termination of the BPM into a coaxial cable, the output signal at the BPM is determined. Thus the issues of signal sensitivity and power output can be addressed quantitatively, including all transient effects and wakefields. Besides this first quantitative analysis of a true BPM 3D structure, they find that internal resonant modes are a major-source of high value narrow-band impedances. The effects of these resonances on coupled-bunch instabilities are discussed. An estimate of the power dissipation in the ceramic vacuum seal under high current operation is given

Simulation of PEP-II Accelerator Backgrounds Using TURTLE

CERN Document Server

Barlow, Roger J; Kozanecki, Witold; Majewski, Stephanie; Roudeau, Patrick; Stocchi, Achille

2005-01-01

We present studies of accelerator-induced backgrounds in the BaBar detector at the SLAC B-Factory, carried out using a modified version ofthe DECAY TURTLE simulation package. Lost-particle backgrounds in PEP-II are dominated by a combination of beam-gas bremstrahlung, beam-gas Coulomb scattering, radiative-Bhabha events and beam-beam blow-up. The radiation damage and detector occupancy caused by the associated electromagnetic shower debris can limit the usable luminosity. In order to understand and mitigate such backgrounds, we have performed a full programme of beam-gas and luminosity-background simulations, that include the effects of the detector solenoidal field, detailed modelling of limiting apertures in both collider rings, and optimization of the betatron collimation scheme in the presence of large transverse tails.

Dual Power Supplies for PEP-II Injection Kickers

CERN Document Server

Olszewski, Joseph; Iverson, Richard; Kulikov, Artem; Pappas, Chris

2005-01-01

Originally the PEP-II injection kickers where powered by one power supply. Since the kicker magnets where not perfectly matched, the stored beam got excited by about 7% of the maximum kicker amplitude. This led to luminosity losses which were especially obvious for trickle injection when the detector is on for data taking. Therefore two independant power supplies with thyratrons in the tunnel next to the kicker magnet were installed. This also reduces the necessary power by about a factor of five since there are no long cables that have to be charged. The kickers are now independantly adjustable to eliminate any non-closure of the kicker system and therefore excitation of the stored beam. Setup, commissioning and fine tuning of this system are discussed.

Dual Power Supplies for PEP-II Injection Kickers

Energy Technology Data Exchange (ETDEWEB)

Olszewski, J; Decker, F.-J.; Iverson, R.H.; Kulikov, A.; Pappas, C.; /SLAC

2005-05-25

Originally the PEP-II injection kickers were powered by one power supply. Since the kicker magnets where not perfectly matched, the stored beam got excited by about 7% of the maximum kicker amplitude. This led to luminosity losses which were especially obvious for trickle injection when the detector is on for data taking. Therefore two independent power supplies with thyratrons in the tunnel next to the kicker magnet were installed. This also reduces the necessary power by about a factor of four since there are no long cables that have to be charged. The kickers are now independently adjustable to eliminate any non-closure of the kicker system and therefore excitation of the stored beam. Setup, commissioning and fine tuning of this system are discussed.

Dual Power Supplies for PEP-II Injection Kickers

International Nuclear Information System (INIS)

Olszewski, J; Decker, F.-J.; Iverson, R.H.; Kulikov, A.; Pappas, C.; SLAC

2005-01-01

Originally the PEP-II injection kickers were powered by one power supply. Since the kicker magnets where not perfectly matched, the stored beam got excited by about 7% of the maximum kicker amplitude. This led to luminosity losses which were especially obvious for trickle injection when the detector is on for data taking. Therefore two independent power supplies with thyratrons in the tunnel next to the kicker magnet were installed. This also reduces the necessary power by about a factor of four since there are no long cables that have to be charged. The kickers are now independently adjustable to eliminate any non-closure of the kicker system and therefore excitation of the stored beam. Setup, commissioning and fine tuning of this system are discussed

On the potential for using immersive virtual environments to support laboratory experiment contextualisation

Science.gov (United States)

Machet, Tania; Lowe, David; Gütl, Christian

2012-12-01

This paper explores the hypothesis that embedding a laboratory activity into a virtual environment can provide a richer experimental context and hence improve the understanding of the relationship between a theoretical model and the real world, particularly in terms of the model's strengths and weaknesses. While an identified learning objective of laboratories is to support the understanding of the relationship between models and reality, the paper illustrates that this understanding is hindered by inherently limited experiments and that there is scope for improvement. Despite the contextualisation of learning activities having been shown to support learning objectives in many fields, there is traditionally little contextual information presented during laboratory experimentation. The paper argues that the enhancing laboratory activity with contextual information affords an opportunity to improve students' understanding of the relationship between the theoretical model and the experiment (which is effectively a proxy for the complex real world), thereby improving their understanding of the relationship between the model and reality. The authors propose that these improvements can be achieved by setting remote laboratories within context-rich virtual worlds.

Exploring the Potential of Smartphones and Tablets for Performance Support in Food Chemistry Laboratory Classes

Science.gov (United States)

van der Kolk, Koos; Hartog, Rob; Beldman, Gerrit; Gruppen, Harry

2013-01-01

Increasingly, mobile applications appear on the market that can support students in chemistry laboratory classes. In a multiple app-supported laboratory, each of these applications covers one use-case. In practice, this leads to situations in which information is scattered over different screens and written materials. Such a multiple app-supported…

PEGASYS---A proposed internal target facility for the PEP storage ring

International Nuclear Information System (INIS)

Van Biber, K.

1988-07-01

A proposal for an integral gas-jet target and forward spectrometer for the PEP storage ring is described. The beam structure, allowable, luminosity (L = 10 33 cm/sup /minus/2/s/sup /minus/1/ for H 2 , D 2 ) and energy (E/sub e/ ≤ 15 GeV) make the ring ideal for multiparticle coincidence studies in the scaling regime, and where perturbative QCD may be an apt description of some exclusive and semi-inclusive reactions. 14 refs., 7 figs

Chemistry and materials science progress report. Weapons-supporting research and laboratory directed research and development: FY 1995

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-04-01

This report covers different materials and chemistry research projects carried out a Lawrence Livermore National Laboratory during 1995 in support of nuclear weapons programs and other programs. There are 16 papers supporting weapons research and 12 papers supporting laboratory directed research.

Chemistry and materials science progress report. Weapons-supporting research and laboratory directed research and development: FY 1995

International Nuclear Information System (INIS)

1996-04-01

This report covers different materials and chemistry research projects carried out a Lawrence Livermore National Laboratory during 1995 in support of nuclear weapons programs and other programs. There are 16 papers supporting weapons research and 12 papers supporting laboratory directed research

Psychoeducation with problem-solving (PEPS) therapy for adults with personality disorder: a pragmatic randomised controlled trial to determine the clinical effectiveness and cost-effectiveness of a manualised intervention to improve social functioning.

Science.gov (United States)

McMurran, Mary; Crawford, Mike J; Reilly, Joe; Delport, Juan; McCrone, Paul; Whitham, Diane; Tan, Wei; Duggan, Conor; Montgomery, Alan A; Williams, Hywel C; Adams, Clive E; Jin, Huajie; Lewis, Matthew; Day, Florence

2016-07-01

availability of 72-week follow-up SFQ data. Median attendance at psychoeducation sessions was approximately 90% and for problem-solving sessions was approximately 50%. PEPS therapy plus usual treatment was no more effective than usual treatment alone for the primary outcome [adjusted difference in means for SFQ -0.73 points, 95% confidence interval (CI) -1.83 to 0.38 points; p = 0.19], any of the secondary outcomes or social problem-solving. Over the follow-up, PEPS costs were, on average, £182 less than for usual treatment. It also resulted in 0.0148 more quality-adjusted life-years. Neither difference was statistically significant. At the National Institute for Health and Care Excellence thresholds, the intervention had a 64% likelihood of being the more cost-effective option. More adverse events, mainly incidents of self-harm, occurred in the PEPS arm, but the difference was not significant (adjusted incidence rate ratio 1.24, 95% CI 0.93 to 1.64). There was possible bias in adverse event recording because of dependence on self-disclosure or reporting by the clinical team. Non-completion of problem-solving sessions and non-standardisation of usual treatment were limitations. We found no evidence to support the use of PEPS therapy alongside standard care for improving social functioning of adults with personality disorder living in the community. We aim to investigate adverse events by accessing centrally held NHS data on deaths and hospitalisation for all PEPS trial participants. Current Controlled Trials ISRCTN70660936. This project was funded by the National Institute for Health Research (NIHR) Health Technology Assessment programme and will be published in full in Health Technology Assessment; Vol. 20, No. 52. See the NIHR Journals Library website for further project information.

Duplicate laboratory test reduction using a clinical decision support tool.

Science.gov (United States)

Procop, Gary W; Yerian, Lisa M; Wyllie, Robert; Harrison, A Marc; Kottke-Marchant, Kandice

2014-05-01

Duplicate laboratory tests that are unwarranted increase unnecessary phlebotomy, which contributes to iatrogenic anemia, decreased patient satisfaction, and increased health care costs. We employed a clinical decision support tool (CDST) to block unnecessary duplicate test orders during the computerized physician order entry (CPOE) process. We assessed laboratory cost savings after 2 years and searched for untoward patient events associated with this intervention. This CDST blocked 11,790 unnecessary duplicate test orders in these 2 years, which resulted in a cost savings of $183,586. There were no untoward effects reported associated with this intervention. The movement to CPOE affords real-time interaction between the laboratory and the physician through CDSTs that signal duplicate orders. These interactions save health care dollars and should also increase patient satisfaction and well-being.

Effect of CO2 enrichment and high photosynthetic photon flux densities (PPFD) on rubisco and PEP-case activities of in vitro cultured strawberry plants

International Nuclear Information System (INIS)

Desjardins, Y.; Beeson, R.; Gosselin, A.

1989-01-01

Standard growing conditions in vitro (low light and CO 2 ) are not conducive to autotrophy. An experiment was conducted to improve photosynthesis in vitro in the hope of increasing survival in acclimatization. A factorial experiment was elaborated where CO 2 and PPFD were supplied to in vitro cultured strawberry plants in the rooting stage. Activities of carboxylating enzymes were determined after 4 weeks of culture. The activities of non-activated and activated rubisco and PEP-Case were measured after extraction of the enzymes and a reaction with NaH 14 CO 3 followed by scintillation counting spectroscopy. High CO 2 concentration significantly increased net assimilation rates (NAR) by 165% over the control for both 1650 and 3000 ppm CO 2 . High PPFD only increased NAR by 12 and 35% for 150 and 250 μmol·m -2 ·s -1 respectively over the control. Plants grown at 3000 ppm CO 2 had the highest level of chlorophyll/g FW with 97% more than the control. The activity of PEP-Case was the highest at high light levels and high CO 2 with rates of 1.65 for 1650 ppm versus 1.22 mmol CO 2 mg -1 chl. h -1 at 250 μmol·m -2 ·s -1 . There was no difference in PEP activity at low light levels. The rubisco activity was lower at 1650 and 3000 ppm CO 2 . Increases in NAR correlate more closely to the PEP-Case than to Rubisco activity. Physiological significance of high activity of PEP-Case over rubisco will be discussed

Building laboratory capacity to support HIV care in Nigeria: Harvard/APIN PEPFAR, 2004-2012.

Science.gov (United States)

Hamel, Donald J; Sankalé, Jean-Louis; Samuels, Jay Osi; Sarr, Abdoulaye D; Chaplin, Beth; Ofuche, Eke; Meloni, Seema T; Okonkwo, Prosper; Kanki, Phyllis J

From 2004-2012, the Harvard/AIDS Prevention Initiative in Nigeria, funded through the US President's Emergency Plan for AIDS Relief programme, scaled up HIV care and treatment services in Nigeria. We describe the methodologies and collaborative processes developed to improve laboratory capacity significantly in a resource-limited setting. These methods were implemented at 35 clinic and laboratory locations. Systems were established and modified to optimise numerous laboratory processes. These included strategies for clinic selection and management, equipment and reagent procurement, supply chains, laboratory renovations, equipment maintenance, electronic data management, quality development programmes and trainings. Over the eight-year programme, laboratories supported 160 000 patients receiving HIV care in Nigeria, delivering over 2.5 million test results, including regular viral load quantitation. External quality assurance systems were established for CD4+ cell count enumeration, blood chemistries and viral load monitoring. Laboratory equipment platforms were improved and standardised and use of point-of-care analysers was expanded. Laboratory training workshops supported laboratories toward increasing staff skills and improving overall quality. Participation in a World Health Organisation-led African laboratory quality improvement system resulted in significant gains in quality measures at five laboratories. Targeted implementation of laboratory development processes, during simultaneous scale-up of HIV treatment programmes in a resource-limited setting, can elicit meaningful gains in laboratory quality and capacity. Systems to improve the physical laboratory environment, develop laboratory staff, create improvements to reduce costs and increase quality are available for future health and laboratory strengthening programmes. We hope that the strategies employed may inform and encourage the development of other laboratories in resource-limited settings.

Operation of the PEP transverse beam feedback

International Nuclear Information System (INIS)

Olson, C.W.; Paterson, J.M.; Pellegrin, J.L.; Rees, J.R.

1981-02-01

The PEP Storage Ring has been equipped with a wide band beam feedback system capable of damping the vertical and horizontal motion of six bunches. The oscillation detection is done at a symmetry point on the Storage Ring and feedback is applied at the same location one orbital period later. The signal is synchronously gated and the system appears as twelve independent feedback loops, operating on the two coordinates of each of the six bunches. Two beam deflection electrodes are driven each by a low-Q push-pull amplifier which is tuned at the 72nd harmonic of the revolution frequency and suppressed-carrier modulation is generated by a sequence of the detected bunch oscillations. The design parameters are reviewed as well as the salient features of the hardware, and the impact of this system on the machine operation is evaluated in the light of experimental results

The PEP-II abort kicker system

International Nuclear Information System (INIS)

Lamare, J de; Donaldson, A.; Kulikov, A. Lipari, J.

1997-07-01

The PEP-II project has two storage rings. The HER (High Energy Ring) has up to 1.48 A of electron beam at 9 GeV, and the LER (Low Energy Ring) has up to 2.14 A of positron beam at 3.1 GeV. To protect the HER and LER beam lines in the event of a ring component failure, each ring has an abort kicker system which directs the beam into a dump when a failure is detected. Due to the high current of the beams, the beam kick is tapered from 100% to 80% in 7.33 uS (the beam transit time around the time). This taper distributes the energy evenly across the window which separates the ring from the beam dump such that the window is not damaged. The abort kicker trigger is synchronized with the ion clearing gap of the beam allowing for the kicker field to rise from 0-80% in 370 nS. This report discusses the design of the system controls, interlocks, power supplies, and modulator

Ba anti Bar status report on the design of a detector for the study of CP violation at PEP-II at SLAC

International Nuclear Information System (INIS)

1993-06-01

Experiments designed to measure CP-violating asymmetries in the B meson system must emphasize exclusive state reconstruction with the highest possible resolution and efficiency. In the case of B 0 mesons produced in pairs at the Υ(4S) resonance at an asymmetric e + e - storage ring, this capability must be accompanied by precise vertex reconstruction in the direction of the e + e - beams (henceforth the z direction). These are difficult requirements, beyond the scope of any existing storage ring detector. The challenge of designing an asymmetric storage ring capable of producing the large number of neutral B meson pairs required to measure CP-violating asymmetries in decays to CP eigenstates has been handsomely met in the PEP-II design. As the first asymmetric e + e - storage ring, PEP-II presents a novel set of experimental challenges to a 4π detector. This Status Report describes the design of a new detector fully capable of exploiting the physics opportunities provided by PEP-II

The effect of positive expiratory pressure (PEP) therapy on symptoms, quality of life and incidence of re-exacerbation in patients with acute exacerbations of chronic obstructive pulmonary disease: a multicentre, randomised controlled trial.

Science.gov (United States)

Osadnik, Christian R; McDonald, Christine F; Miller, Belinda R; Hill, Catherine J; Tarrant, Ben; Steward, Ranjana; Chao, Caroline; Stodden, Nicole; Oliveira, Cristino C; Gagliardi, Nadia; Holland, Anne E

2014-02-01

Positive expiratory pressure (PEP) is a technique used to enhance sputum clearance during acute exacerbations of chronic obstructive pulmonary disease (AECOPD). The impact of PEP therapy during acute exacerbations on clinically important outcomes is not clear. This study sought to determine the effect of PEP therapy on symptoms, quality of life and future exacerbations in patients with AECOPD. 90 inpatients (58 men; mean age 68.6 years, FEV(1) 40.8% predicted) with AECOPD and sputum expectoration were randomised to receive usual care (including physical exercise)±PEP therapy. The Breathlessness, Cough and Sputum Scale (BCSS), St George's Respiratory Questionnaire (SGRQ) and BODE index (Body mass index, airflow Obstruction, Dyspnoea, Exercise tolerance) were measured at discharge, 8 weeks and 6 months following discharge, and analysed via linear mixed models. Exacerbations and hospitalisations were recorded using home diaries. There were no significant between-group differences over time for BCSS score [mean (SE) at discharge 5.2 (0.4) vs 5.0 (0.4) for PEP and control group, respectively; p=0.978] or SGRQ total score [41.6 (2.6) vs 40.8 (2.8) at 8 weeks, p=0.872]. Dyspnoea improved more rapidly in the PEP group over the first 8 weeks (p=0.006), however these benefits were not observed at 6 months. Exacerbations (p=0.986) and hospitalisations (p=0.359) did not differ between groups. We found no evidence that PEP therapy during AECOPD improves important short-term or long-term outcomes. There does not appear to be a routine role for PEP therapy in the management of such individuals.

AutoLabDB: a substantial open source database schema to support a high-throughput automated laboratory.

Science.gov (United States)

Sparkes, Andrew; Clare, Amanda

2012-05-15

Modern automated laboratories need substantial data management solutions to both store and make accessible the details of the experiments they perform. To be useful, a modern Laboratory Information Management System (LIMS) should be flexible and easily extensible to support evolving laboratory requirements, and should be based on the solid foundations of a robust, well-designed database. We have developed such a database schema to support an automated laboratory that performs experiments in systems biology and high-throughput screening. We describe the design of the database schema (AutoLabDB), detailing the main features and describing why we believe it will be relevant to LIMS manufacturers or custom builders. This database has been developed to support two large automated Robot Scientist systems over the last 5 years, where it has been used as the basis of an LIMS that helps to manage both the laboratory and all the experiment data produced.

The PEP-II Lower Pressure HER Vacuum Chamber

International Nuclear Information System (INIS)

DeBarger, S.; Metcalfe, S.; Seeman, J.; Sullivan, M.; Wienands, U.; Wright, D.

2006-01-01

This new vacuum chamber has been installed from 12 to 21 meters upstream of the BaBar detector in the PEP-II High Energy Ring (HER) to reduce lost particle backgrounds. The backgrounds from HER now dominate the backgrounds in the BaBar detector and the present vacuum pressure is 1 x 10 -9 Torr. The new chamber will increase the pumping significantly by adding 18 x 2000 l/s titanium sublimation pumps to the existing 5 x 440 l/s ion pumps, and is expected to reduce the pressure by about a factor of five. Features of the chamber include improved water cooling, improved vacuum conductance through copper RF screens featuring over 15,000 small square holes and the ability to sublimate titanium while the beam is still on

The electron-cloud instability in PEP-II: An update

International Nuclear Information System (INIS)

Furman, M.A.; Lambertson, G.R.

1997-05-01

The authors present an update on the estimate of the growth time of the multi-bunch transverse instability in the PEP-II collider arising from the interaction of the positron beam with the accumulated electron cloud. They estimate the contributions to the growth rate arising from the dipole magnets and from the pumping straight sections. They emphasize those quantities upon which the instability is most sensitive. The simulation includes measured data on the secondary emission yield for TiN-coated samples of the actual vacuum chamber. Although the analysis is still in progress, they conclude that the instability risetime is of order 1 ms, which is well within the range controllable by the feedback system

Coherent x-rays from PEP

International Nuclear Information System (INIS)

Baird, S.; Nuhn, H.-D.; Tatchyn, R.; Winick, H.; Fisher, A.S.; Gallardo, J.C.; Pellegrini, C.

1991-01-01

This paper explores the use of a large-circumference, high-energy, electron-positron collider such as PEP to drive a free-electron laser (FEL), producing high levels of coherent power at short wavelengths. The author consider Self-Amplified Spontaneous Emission (SASE), in which electron bunches with low emittance, high peak current and small energy spread radiate coherently in a single passthrough a long undulator. As the electron beam passes down the undulator, its interaction with the increasingly intense spontaneous radiation causes a bunch density modulation at the optical wavelength, resulting in stimulated emissional growth of coherent power in a single pass. The need for optical-cavity mirrors, which place a lower limit on the wavelength of a conventional FEL oscillator, is avoided. The authors explore various combinations of electron-beam and undulator parameters, as well as special undulator designs and optical klystrons (OK), to reach high average or peak coherent power at wavelengths around 40 angstrom by achieving significant exponential gain or full saturation. Examples are presented for devices that achieve high peak coherent power (up to about 400 MW) with lower average coherent power (about 20 mW) and other devices which produce a few watts of average coherent power

Process Waste Assessment for the Plotting and Digitizing Support Laboratory

International Nuclear Information System (INIS)

Phillips, N.M.

1994-04-01

This Process Waste Assessment was conducted to evaluate the Plotting and Digitizing Support Laboratory, located in Building 913, Room 157. It documents the processes, identifies the hazardous chemical waste streams generated by these processes, recommends possible ways to minimize waste, and serves as a reference for future assessments of this facility

Effects of a School-Based Sexuality Education Program on Peer Educators: The Teen PEP Model

Science.gov (United States)

Jennings, J. M.; Howard, S.; Perotte, C. L.

2014-01-01

This study evaluated the impact of the Teen Prevention Education Program (Teen PEP), a peer-led sexuality education program designed to prevent unintended pregnancy and sexually transmitted infections (STIs) including HIV among high school students. The study design was a quasi-experimental, nonrandomized design conducted from May 2007 to May…

The efficacy of chimeric vaccines constructed with PEP-1 and Ii-Key linking to a hybrid epitope from heterologous viruses.

Science.gov (United States)

Liu, Xue-lan; Shan, Wen-jie; Xu, Shan-shan; Zhang, Jin-jing; Xu, Fa-zhi; Xia, Sheng-lin; Dai, Yin

2015-09-01

The heterologous epitope-peptide from different viruses may represent an attractive candidate vaccine. In order to evaluate the role of cell-permeable peptide (PEP-1) and Ii-Key moiety from the invariant chain (Ii) of MHC on the heterologous peptide chimeras, we linked the two vehicles to hybrid epitopes on the VP2 protein (aa197-209) of the infectious bursal disease virus and HN protein (aa345-353) of the Newcastle disease virus. The chimeric vaccines were prepared and injected into mice. The immune effects were measured by indirect ELISA. The results showed that the vehicle(s) could significantly boost immune effects against the heterologous epitope peptide. The Ii-Key-only carrier induced more effective immunological responses, compared with the PEP-1 and Ii-Key hybrid vehicle. The carrier-peptide hybrids all showed strong colocalization with major histocompatibility complex (MHC) class II molecules compared with the epitope-peptide (weakly-binding) after co-transfection into 293T cells. Together, our results lay the groundwork for designing new hybrid vaccines based on Ii-Key and/or PEP-1 peptides. Copyright © 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

Injection system for the PEP II asymmetric B Factory at SLAC

International Nuclear Information System (INIS)

Fieguth, T.; Bloom, E.; Bulos, F.; Davies-White, W.; Donald, M.; Fairfield, K.; Godfrey, G.; Holtzapple, R.; Ronan, M.; Zisman, M.

1992-01-01

The asymmetric energy B Factory proposed as an upgrade of PEP at Stanford Linear Accelerator Center will require a highly reliable and efficient injection system. The conceptual design has shown the feasibility of extracting 9 GeV electrons and 3.1 GeV positrons from the existing linac and injecting equal charges into 1658 buckets in each of the two rings of the collider. An injection study group has continued the development and study of this proposal and has generated workable designs for many related systems and subsystems. (author) 3 refs.; 4 figs.; 2 tabs

Final report of the Multiprogram Laboratory Panel Energy Research Advisory Board. Volume II. Support studies

International Nuclear Information System (INIS)

Spiewak, I.; Guthrie, M.P.; Nichols, J.P.; Preston, E.L.; West, C.D.; Wilbanks, T.J.; Wilkes, B.Y.; Zerby, A.C.

1982-09-01

Volume II - support studies for nine national laboratories include: report of statistical data on the multiprogram laboratories; examples of national laboratory use in foreign countries; domestic models for national laboratory utilization; relationships of laboratories with industry and universities; uses of laboratories for training industrial R and D personnel; legal mandates and constraints on the national laboratories; with appendices on facts about Harwell, CEN-Saclay, TNO, Studsvik, and JAERI-Tokai; the Requirements Boards of the United Kingdom Department of Industry; impact of President's FY 1983 budget; and the PNL experiment

Nuclab Marcoule: a dedicated waste management and dismantling support laboratory

International Nuclear Information System (INIS)

Dugne, Olivier; Bec-Espitalier, Lionel; Rosen, Jeremy

2014-01-01

Formerly dedicated to plutonium production support, NucLab was renovated to perform a wide range of analyses for dismantling, plant operation and process development activities mainly at Marcoule but also for external clients. The laboratory is a CEA entity in the Nuclear Energy Division. It provides services to several industrial operators (nuclear processes and power plants) in the fields of analytical chemistry, radioactivity measurements, in situ nuclear measurements, decontamination processes, industrial chemistry processes, and waste treatment. NucLab supports research, production, and dismantling activities in all areas of dismantling operations (authors)

ELAN - expert system supported information and management system for analytical laboratories. ELAN - Expertengestuetztes Informationssystem fuer die Laboranalytik

Energy Technology Data Exchange (ETDEWEB)

Orth, H.; Zilly, G.

1990-05-01

The demand for high efficiency and short response time calls for the use of computer support in chemico-analytical laboratories. This is usually achieved by laboratory information and management systems covering the three levels of analytical instrument automation, laboratory operation support and laboratory management. The management component of the systems implemented up to now suffers from a lack of flexibility as far as unforeseen analytical investigations outside the laboratory routine work are concerned. Another drawback is the lack of adaptability with respect to structural changes in laboratory organization. It can be eliminated by the application of expert system structures and methods for the implementation of this system level. The ELAN laboratory information and management system has been developed on the basis of this concept. (orig.).

Secondary Electron Yield Measurements and Groove Chambers Tests in the PEP-II Beam Line Straights Sections

International Nuclear Information System (INIS)

Pivi, M

2008-01-01

Beam instability caused by the electron cloud has been observed in positron and proton storage rings and it is expected to be a limiting factor in the performance of the positron Damping Ring (DR) of future Linear Colliders such as ILC and CLIC [1, 2]. In the Positron Low Energy Ring (LER) of the PEP-II accelerator, we have installed vacuum chambers with rectangular grooves in a straight magnetic-free section to test this promising possible electron cloud mitigation technique. We have also installed a special chamber to monitor the secondary electron yield of TiN and TiZrV (NEG) coating, Copper, Stainless Steel and Aluminum under the effect of electron and photon conditioning in situ in the beam line. In this paper, we describe the ongoing R and D effort to mitigate the electron cloud effect for the ILC damping ring, the latest results on in situ secondary electron yield conditioning and recent update on the groove tests in PEP-II

A proposed orbit and vertical dispersion correction system for PEP

International Nuclear Information System (INIS)

Close, E.; Cornacchia, M.; King, A.S.; Lee, M.J.

1978-07-01

The proposed arrangement of position monitors and dipole magnets for the closed orbit correction system in PEP is described. The computer code ALIGN, which simulates and corrects closed orbit displacements, has been used to study the most effective layout of monitors and correctors. The vertical dispersion function has been computed before and after closed orbit correction. The results indicate that the residual vertical dispersion after the orbit is corrected could exceed the tolerable values. A correction procedure for the vertical dispersion has been studied with the compute code CO-OP and this scheme of correction has been verified experimentally in SPEAR. 9 refs., 8 figs., 2 tabs

Commissioning experience from PEP-II HER longitudinal feedback

International Nuclear Information System (INIS)

Prabhakar, S.; Teytelman, D.; Fox, J.; Young, A.; Corredoura, P.; Tighe, R.

1998-06-01

The DSP-based bunch-by-bunch feedback system installed in the PEP-II HER has been used to damp HOM-induced instabilities at beam currents up to 6-5 mA during commissioning. Beam pseudospectra calcualted from feedback system data indicate the presence of coupled bunch modes that oincide with the 0-M-2 cavity HOM. Bunch current and synchronous phase measurements are also extracted from the data. These measurements reveal the impedance seen by the beam at revolution harmonics. The impedance peak at 3*frev indicates incorrect parking of the idle cavities, and explains the observed instability of mode 3. Bunch synchrotron tunes are calculated from lorentzian fits to the data. Bunch-to-bunch time variation due to the cavity transient is shown to be large enough to result in Landau damping of coupled bunch modes

Building laboratory capacity to support HIV care in Nigeria: Harvard/APIN PEPFAR, 2004–2012

Science.gov (United States)

Hamel, Donald J.; Sankalé, Jean-Louis; Samuels, Jay Osi; Sarr, Abdoulaye D.; Chaplin, Beth; Ofuche, Eke; Meloni, Seema T.; Okonkwo, Prosper; Kanki, Phyllis J.

2015-01-01

Introduction From 2004–2012, the Harvard/AIDS Prevention Initiative in Nigeria, funded through the US President’s Emergency Plan for AIDS Relief programme, scaled up HIV care and treatment services in Nigeria. We describe the methodologies and collaborative processes developed to improve laboratory capacity significantly in a resource-limited setting. These methods were implemented at 35 clinic and laboratory locations. Methods Systems were established and modified to optimise numerous laboratory processes. These included strategies for clinic selection and management, equipment and reagent procurement, supply chains, laboratory renovations, equipment maintenance, electronic data management, quality development programmes and trainings. Results Over the eight-year programme, laboratories supported 160 000 patients receiving HIV care in Nigeria, delivering over 2.5 million test results, including regular viral load quantitation. External quality assurance systems were established for CD4+ cell count enumeration, blood chemistries and viral load monitoring. Laboratory equipment platforms were improved and standardised and use of point-of-care analysers was expanded. Laboratory training workshops supported laboratories toward increasing staff skills and improving overall quality. Participation in a World Health Organisation-led African laboratory quality improvement system resulted in significant gains in quality measures at five laboratories. Conclusions Targeted implementation of laboratory development processes, during simultaneous scale-up of HIV treatment programmes in a resource-limited setting, can elicit meaningful gains in laboratory quality and capacity. Systems to improve the physical laboratory environment, develop laboratory staff, create improvements to reduce costs and increase quality are available for future health and laboratory strengthening programmes. We hope that the strategies employed may inform and encourage the development of other

Transitioning to HIV Pre-Exposure Prophylaxis (PrEP) from Non-Occupational Post-Exposure Prophylaxis (nPEP) in a Comprehensive HIV Prevention Clinic: A Prospective Cohort Study.

Science.gov (United States)

Siemieniuk, Reed A C; Sivachandran, Nirojini; Murphy, Pauline; Sharp, Andrea; Walach, Christine; Placido, Tania; Bogoch, Isaac I

2015-08-01

The uptake of pre-exposure prophylaxis (PrEP) for HIV prevention remains low. We hypothesized that a high proportion of patients presenting for HIV non-occupational post-exposure prophylaxis (nPEP) would be candidates for PrEP based on current CDC guidelines. Outcomes from a comprehensive HIV Prevention Clinic are described. We evaluated all patients who attended the HIV Prevention Clinic for nPEP between January 1, 2013 and September 30, 2014. Each patient was evaluated for PrEP candidacy based on current CDC-guidelines and subjectively based on physician opinion. Patients were then evaluated for initiation of PrEP if they met guideline suggestions. Demographic, social, and behavioral factors were then analyzed with logistic regression for associations with PrEP candidacy and initiation. 99 individuals who attended the nPEP clinic were evaluated for PrEP. The average age was 32 years (range, 18-62), 83 (84%) were male, of whom 46 (55%) men who had have sex with men (MSM). 31 (31%) met CDC guidelines for PrEP initiation, which had very good agreement with physician recommendation (kappa=0.88, 0.78-0.98). Factors associated with PrEP candidacy included sexual exposure to HIV, prior nPEP use, and lack of drug insurance (p<0.05 for all comparisons). Combining nPEP and PrEP services in a dedicated clinic can lead to identification of PrEP candidates and may facilitate PrEP uptake. Strategies to ensure equitable access of PrEP should be explored such that those without drug coverage may also benefit from this effective HIV prevention modality.

Local chromatic correction scheme for LER of PEP-II

International Nuclear Information System (INIS)

Forest, E.; Robin, D.; Zholents, A.; Donald, M.; Helm, R.; Irwin, J.; Sullivan, M.K.

1993-01-01

The correction of the chromaticity of low-beta insertions in storage rings is usually made with sextupole lenses in the ring arcs. When decreasing the beta functions at the interaction point (IP), this technique becomes fairly ineffective, since it fails to properly correct the higher-order chromatic aberrations. Here we consider the approach for ampersand PEP-II B Factory low energy ring (LER) where the chromatic effects of the quadrupole lenses generating low beta functions at the IP are corrected locally with two families of sextupoles, one family for each plane. For the IP straight section the lattice is designed in such a way that the chromatic aberrations are made small and sextupole-like aberrations are eliminated. The results of dimensional tracking simulations are presented

High-power RF window and coupler development for the PEP-II B Factory

International Nuclear Information System (INIS)

Neubauer, M.; Fant, K.; Hodgson, J.; Judkins, J.; Schwarz, H.; Rimmer, R.A.

1995-05-01

We describe the fabrication and testing of the RF windows designed to transmit power to the PEP-II 476 MHz cavities. Design choices to maximize the reliability of the window are discussed. Fabrication technologies for the window are described and finite-element analysis of the assembly process is presented. Conditioning and high-power testing of the window are discussed. Design of the coupler assembly including the integration of the window and other components is reported

Diagnosing the PEP-II Injection System

Energy Technology Data Exchange (ETDEWEB)

Decker, F.-J.; Donald, M.H.; Iverson, R.H.; Kulikov, A.; Pappas, G.C.; Weaver, M.; /SLAC

2005-05-09

The injection of beam into the PEP-II B-Factory, especially into the High Energy Ring (HER) has some challenges. A high background level in the BaBar detector has for a while inhibited us from trickling charge into the HER similar to the Low Energy Ring (LER). Analyzing the injection system has revealed many issues which could be improved. The injection bump between two kickers was not closed, mainly because the phase advance wasn't exactly 180{sup o} and the two kicker strengths were not balanced. Additionally we found reflections which kick the stored beam after the main kick and cause the average luminosity to drop about 3% for a 10 Hz injection rate. The strength of the overall kick is nearly twice as high as the design, indicating a much bigger effective septum thickness. Compared with single beam the background is worse when the HER beam is colliding with the LER beam. This hints that the beam-beam force and the observed vertical blow-up in the HER pushes the beam and especially the injected beam further out to the edge of the dynamic aperture or beyond.

Diagnosing the PEP-II Injection System

International Nuclear Information System (INIS)

Decker, F.-J.; Donald, M.H.; Iverson, R.H.; Kulikov, A.; Pappas, G.C.; Weaver, M.; SLAC

2005-01-01

The injection of beam into the PEP-II B-Factory, especially into the High Energy Ring (HER) has some challenges. A high background level in the BaBar detector has for a while inhibited us from trickling charge into the HER similar to the Low Energy Ring (LER). Analyzing the injection system has revealed many issues which could be improved. The injection bump between two kickers was not closed, mainly because the phase advance wasn't exactly 180 o and the two kicker strengths were not balanced. Additionally we found reflections which kick the stored beam after the main kick and cause the average luminosity to drop about 3% for a 10 Hz injection rate. The strength of the overall kick is nearly twice as high as the design, indicating a much bigger effective septum thickness. Compared with single beam the background is worse when the HER beam is colliding with the LER beam. This hints that the beam-beam force and the observed vertical blow-up in the HER pushes the beam and especially the injected beam further out to the edge of the dynamic aperture or beyond

Implications of shorter cells in PEP

International Nuclear Information System (INIS)

Wiedemann, H.

1975-01-01

Further studies on the beam-stay-clear requirements in PEP led to the conclusion that the vertical aperture needed to be enlarged. There are two main reasons for that: Observations at SPEAR indicate that the aperture should be large enough for a fully coupled beam. Full coupling of the horizontal and vertical betatron oscillations occurs not only occasionally when the energy, tune or betatron function at the interaction point is changed but also due to the beam/endash/beam effect of two strong colliding beams. The second reason for an increased aperture requirement is the nonlinear perturbation of the particle trajectories by the sextupoles. This perturbation increases a fully coupled beam by another 50% to 80%. Both effects together with a +-5 mm allowance for closed orbit perturbation result in a vertical beam-stay-clear in the bending magnets of +-4.8 to +-5.6 cm, compared to the present +-2.0 cm. This beam-stay-clear, together with additional space for vacuum chamber, etc., leads to very costly bending magnets. In this note, a shorter cell length is proposed which would reduce considerably the vertical beam-stay-clear requirements in the bending magnets. 7 figs

Accelerator-based techniques for the support of senior-level undergraduate physics laboratories

International Nuclear Information System (INIS)

Williams, J.R.; Clark, J.C.; Isaacs-Smith, T.

2001-01-01

Approximately three years ago, Auburn University replaced its aging Dynamitron accelerator with a new 2MV tandem machine (Pelletron) manufactured by the National Electrostatics Corporation (NEC). This new machine is maintained and operated for the University by Physics Department personnel, and the accelerator supports a wide variety of materials modification/analysis studies. Computer software is available that allows the NEC Pelletron to be operated from a remote location, and an Internet link has been established between the Accelerator Laboratory and the Upper-Level Undergraduate Teaching Laboratory in the Physics Department. Additional software supplied by Canberra Industries has also been used to create a second Internet link that allows live-time data acquisition in the Teaching Laboratory. Our senior-level undergraduates and first-year graduate students perform a number of experiments related to radiation detection and measurement as well as several standard accelerator-based experiments that have been added recently. These laboratory exercises will be described, and the procedures used to establish the Internet links between our Teaching Laboratory and the Accelerator Laboratory will be discussed

PEP quark search proportional chambers

Energy Technology Data Exchange (ETDEWEB)

Parker, S I; Harris, F; Karliner, I; Yount, D [Hawaii Univ., Honolulu (USA); Ely, R; Hamilton, R; Pun, T [California Univ., Berkeley (USA). Lawrence Berkeley Lab.; Guryn, W; Miller, D; Fries, R [Northwestern Univ., Evanston, IL (USA)

1981-04-01

Proportional chambers are used in the PEP Free Quark Search to identify and remove possible background sources such as particles traversing the edges of counters, to permit geometric corrections to the dE/dx and TOF information from the scintillator and Cerenkov counters, and to look for possible high cross section quarks. The present beam pipe has a thickness of 0.007 interaction lengths (lambdasub(i)) and is followed in both arms each with 45/sup 0/ <= theta <= 135/sup 0/, ..delta..phi=90/sup 0/ by 5 proportional chambers, each 0.0008 lambdasub(i) thick with 32 channels of pulse height readout, and by 3 thin scintillator planes, each 0.003 lambdasub(i) thick. Following this thin front end, each arm of the detector has 8 layers of scintillator (one with scintillating light pipes) interspersed with 4 proportional chambers and a layer of lucite Cerenkov counters. Both the calculated ion statistics and measurements using He-CH/sub 4/ gas in a test chamber indicate that the chamber efficiencies should be >98% for q=1/3. The Landau spread measured in the test was equal to that observed for normal q=1 traversals. One scintillator plane and thin chamber in each arm will have an extra set of ADC's with a wide gate bracketing the normal one so timing errors and tails of earlier pulses should not produce fake quarks.

A Living Laboratory Exploring Mobile Support for Everyday Life with Diabetes

DEFF Research Database (Denmark)

Kanstrup, Anne Marie; Bjerge, Kim; Kristensen, Jens E.

2010-01-01

The paper presents the set up of a Living Laboratory in a city of North Denmark exploring mobile support for everyday life with diabetes. Background and definitions of the living lab method is presented together with descriptions of the technical setup, applications and explorations. The living l...

Building laboratory capacity to support HIV care in Nigeria: Harvard/APIN PEPFAR, 2004–2012

Directory of Open Access Journals (Sweden)

Donald J. Hamel

2015-05-01

Full Text Available Introduction: From 2004–2012, the Harvard/AIDS Prevention Initiative in Nigeria, funded through the US President’s Emergency Plan for AIDS Relief programme, scaled up HIV care and treatment services in Nigeria. We describe the methodologies and collaborative processes developed to improve laboratory capacity significantly in a resource-limited setting. These methods were implemented at 35 clinic and laboratory locations. Methods: Systems were established and modified to optimise numerous laboratory processes. These included strategies for clinic selection and management, equipment and reagent procurement, supply chains, laboratory renovations, equipment maintenance, electronic data management, quality development programmes and trainings. Results: Over the eight-year programme, laboratories supported 160 000 patients receiving HIV care in Nigeria, delivering over 2.5 million test results, including regular viral load quantitation. External quality assurance systems were established for CD4+ cell count enumeration, blood chemistries and viral load monitoring. Laboratory equipment platforms were improved and standardised and use of point-of-care analysers was expanded. Laboratory training workshops supported laboratories toward increasing staff skills and improving overall quality. Participation in a World Health Organisation-led African laboratory quality improvement system resulted in significant gains in quality measures at five laboratories. Conclusions: Targeted implementation of laboratory development processes, during simultaneous scale-up of HIV treatment programmes in a resource-limited setting, can elicit meaningful gains in laboratory quality and capacity. Systems to improve the physical laboratory environment, develop laboratory staff, create improvements to reduce costs and increase quality are available for future health and laboratory strengthening programmes. We hope that the strategies employed may inform

Computational tools and lattice design for the PEP-II B-Factory

International Nuclear Information System (INIS)

Cai Yunhai; Irwin, John; Nosochkov, Yuri; Yan, Yiton

1997-01-01

Several accelerator codes were used to design the PEP-II lattices, ranging from matrix-based codes, such as MAD and DIMAD, to symplectic-integrator codes, such as TRACY and DESPOT. In addition to element-by-element tracking, we constructed maps to determine aberration strengths. Furthermore, we have developed a fast and reliable method (nPB tracking) to track particles with a one-turn map. This new technique allows us to evaluate performance of the lattices on the entire tune-plane. Recently, we designed and implemented an object-oriented code in C++ called LEGO which integrates and expands upon TRACY and DESPOT

Perspectives on HIV Pre- and Post-Exposure Prophylaxes (PrEP and PEP) Among Female and Male Sex Workers in Mombasa, Kenya: Implications for Integrating Biomedical Prevention into Sexual Health Services.

Science.gov (United States)

Restar, Arjee J; Tocco, Jack Ume; Mantell, Joanne E; Lafort, Yves; Gichangi, Peter; Masvawure, Tsitsi B; Chabeda, Sophie Vusha; Sandfort, Theo G M

2017-04-01

Pre- and post-exposure prophylaxes (PrEP and PEP) can reduce the risk of HIV acquisition, yet often are inaccessible to and underutilized by most-vulnerable populations, including sex workers in sub-Saharan Africa. Based on in-depth interviews with 21 female and 23 male HIV-negative sex workers in Mombasa, Kenya, we found that awareness and knowledge of PrEP and PEP were low, although willingness to use both was high. Participants felt PrEP would be empowering and give added protection against infection, although some expressed concerns about side effects. Despite PEP's availability, few knew about it and even fewer had used it, but most who had would use it again. Sex workers valued confidentiality, privacy, trustworthiness, and convenient location in health services and wanted thorough HIV/STI assessments. These findings suggest the importance of situating PrEP and PEP within sex worker-friendly health services and conducting outreach to promote these biomedical prevention methods for Kenyan sex workers.

Quality assurance plan for the Close Support Laboratory for the remedial investigation at Oak Ridge National Laboratory, Oak Ridge, Tennessee

International Nuclear Information System (INIS)

1993-08-01

The primary purpose of the Close Support Laboratory (CSL) is to provide rapid radiological screening of investigation-derived samples before they are shipped to off-site laboratories for more detailed analyses. Analyses for volatile organic compounds and miscellaneous water quality parameters are also performed at the CSL. CSL data are also used to select samples for off-site laboratory analysis, for rapid qualitative and quantitative determinations, and for other processes when off-site analysis is not needed and/or is impractical. This plan specifies methods of implementing analytical and radiological protocols and procedures for the documentation, handling, control, and analysis of samples and describes the levels of authority and responsibility for laboratory operation. Specific quality control methods used by the CSL for individual analyses are described in project procedures

PEP II: RF and feedback R ampersand D

International Nuclear Information System (INIS)

Hindi, H.: Fox, J.; Eisen, N.; Linscott, I.; Oxoby, G.; Pendleton, R.; Sapozhnikov, L.; Schwarz, H.D.; Rimmer, R.; Lambertson, A.; Voelker, F.; Serio, M.

1992-11-01

The key elements of the PEP 11 approach to dealing with the problem of coupled-bunch instabilities are presented. The approach involves using warm copper RF cavities with damping waveguides (above the cutoff of the fundamental) attached to the cavity walls. These couple out the troublesome higher-order modes (HOMs) into absorbing loads, while the fundamental mode remains in the cavity. The Q of the worst HOM is reduced to below 70. Instabilities due to residual coupling are damped using a bunch-by-bunch feedback system, which is implemented using digital signal processors. A prototype cavity has been built and the concept of HOM damping has been verified. A prototype feedback system has been built and tested at SPEAR. Results to date indicate that the combination of damped cavities and bunch-by-bunch feedback provide a very effective means of dealing with the problem

Hawaiian Electric Advanced Inverter Grid Support Function Laboratory Validation and Analysis

Energy Technology Data Exchange (ETDEWEB)

Nelson, Austin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Nagarajan, Adarsh [National Renewable Energy Lab. (NREL), Golden, CO (United States); Prabakar, Kumar [National Renewable Energy Lab. (NREL), Golden, CO (United States); Gevorgian, Vahan [National Renewable Energy Lab. (NREL), Golden, CO (United States); Lundstrom, Blake [National Renewable Energy Lab. (NREL), Golden, CO (United States); Nepal, Shaili [National Renewable Energy Lab. (NREL), Golden, CO (United States); Hoke, Anderson [National Renewable Energy Lab. (NREL), Golden, CO (United States); Asano, Marc [Hawaiian Electric Company, Honolulu, HI (United States); Ueda, Reid [Hawaiian Electric Company, Honolulu, HI (United States); Shindo, Jon [Hawaiian Electric Company, Honolulu, HI (United States); Kubojiri, Kandice [Hawaiian Electric Company, Honolulu, HI (United States); Ceria, Riley [Hawaiian Electric Company, Honolulu, HI (United States); Ifuku, Earle [Hawaiian Electric Company, Honolulu, HI (United States)

2016-12-01

The objective for this test plan was to better understand how to utilize the performance capabilities of advanced inverter functions to allow the interconnection of distributed energy resource (DER) systems to support the new Customer Self-Supply, Customer Grid-Supply, and other future DER programs. The purpose of this project was: 1) to characterize how the tested grid supportive inverters performed the functions of interest, 2) to evaluate the grid supportive inverters in an environment that emulates the dynamics of O'ahu's electrical distribution system, and 3) to gain insight into the benefits of the grid support functions on selected O'ahu island distribution feeders. These goals were achieved through laboratory testing of photovoltaic inverters, including power hardware-in-the-loop testing.

Status of the PEP-II B-factory high energy ring

International Nuclear Information System (INIS)

Wienands, U.; Reuter, E.; Bellomo, P.; Daly, E.; Fisher, A.; Gracia, J.; Kulikov, A.; Kurita, N.; Pietryka, M.; Seeman, J.T.; Taylor; Belser, C.; Bertolini, L.; Mugge, M.; Swan, J.

1996-01-01

The 9 GeV High Energy Ring (HER) of the PEP-II B Factory is an electron storage ring under construction at SLAC. Significant progress has been made in the last year on all systems. As of mid 1996, all 192 dipoles have been installed, with installation of the quadrupoles underway. The vacuum system, for design currents up to 3 A average, is in production using a recently commissioned e-beam welder. Beam instrumentation systems are being fabricated. The interaction region will bring the HER beam into collision with the 3 GeV beam of the Low Energy Ring; design of this section of the HER is in an advanced stage. 8 refs., 3 figs., 1 tab

Beam Commissioning of the PEP-II High Energy Ring

International Nuclear Information System (INIS)

Wienands, U.; Anderson, S.; Assmann, R.; Bharadwaj, V.; Cai, Y.; Clendenin, J.; Corredoura, P.; Decker, F.J.; Donald, M.; Ecklund, S.; Emma, P.; Erickson, R.; Fox, J.; Fieguth, T.; Fisher, A.; Heifets, S.; Hill, A.; Himel, T.; Iverson, R.; Johnson, R.; Judkins, J.; Krejcik, P.; Kulikov, A.; Lee, M.; Mattison, T.; Minty, M.; Nosochkov, Y.; Phinney, N.; Placidi, M.; Prabhakar, S.; Ross, M.; Smith, S.; Schwarz, H.; Stanek, M.; Teytelman, D.; Traller, R.; Turner, J.; Zimmermann, F.; Barry, W.; Chattopadhyay, S.; Corlett, J.; Decking, W.; Furman, M.; Nishimura, H.; Portmann, G.; Rimmer, R.; Zholents, A.; Zisman, M.; Kozanecki, W.; Hofmann, A.; Zotter, B.; Steier, C.; Bialowons, W.; Lomperski, M.; Lumpkin, A.; Reichel, I.; Safranek, J.; Smith, V.; Tighe, R.; Sullivan, M.; Byrd, J.; Li, D.

1998-01-01

The PEP-II High Energy Ring (HER), a 9 GeV electron storage ring, has been in commissioning since spring 1997. Initial beam commissioning activities focused on systems checkout and commissioning and on determining the behavior of the machine systems at high beam currents. This phase culminated with the accumulation of 0.75 A of stored beam-sufficient to achieve design luminosity--in January 1998 after 3.5 months of beam time. Collisions with the 3 GeV positron beam of the Low Energy Ring (LER) were achieved in Summer of 1998. At high beam currents, collective instabilities have been seen. Since then, commissioning activities for the HER have shifted in focus towards characterization of the machine and a rigorous program to understand the machine and the beam dynamics is presently underway

Detector solenoid compensation in the PEP-II B-Factory

International Nuclear Information System (INIS)

Nosochkov, Y.; Cai, Y.; Irwin, J.; Sullivan, M.

1995-01-01

The PEP-II experimental detector includes a strong 1.5 T solenoid field in the interaction region (IR). With the fringe fields, the solenoid extends over a range of 6 m. Additional complications are that (1) it is displaced longitudinally from the interaction point (IP) by about 40 cm, (2) neither beam is parallel to the solenoid axis, and (3) the solenoid overlaps a dipole and a quadrupole on either side of the IP. In each half IR the correction system includes a set of skew quadrupoles, dipole correctors and normal quadrupoles to independently compensate the coupling, orbit perturbation, dispersion and focusing effect produced by the solenoid. The correction schemes for the Low Energy Ring (LER) and for the High Energy Ring (HER) are described, and the impact on the dynamic aperture is evaluated

Ba{anti B}ar status report on the design of a detector for the study of CP violation at PEP-II at SLAC

Energy Technology Data Exchange (ETDEWEB)

1993-06-01

Experiments designed to measure CP-violating asymmetries in the B meson system must emphasize exclusive state reconstruction with the highest possible resolution and efficiency. In the case of B{sup 0} mesons produced in pairs at the {Upsilon}(4S) resonance at an asymmetric e{sup +}e{sup {minus}} storage ring, this capability must be accompanied by precise vertex reconstruction in the direction of the e{sup +}e{sup {minus}} beams (henceforth the z direction). These are difficult requirements, beyond the scope of any existing storage ring detector. The challenge of designing an asymmetric storage ring capable of producing the large number of neutral B meson pairs required to measure CP-violating asymmetries in decays to CP eigenstates has been handsomely met in the PEP-II design. As the first asymmetric e{sup +}e{sup {minus}} storage ring, PEP-II presents a novel set of experimental challenges to a 4{pi} detector. This Status Report describes the design of a new detector fully capable of exploiting the physics opportunities provided by PEP-II.

Dynamic Aperture Improvement of PEP-II Lattices Using Resonance Basis Lie Generators

Energy Technology Data Exchange (ETDEWEB)

Yan, Yiton T

2003-08-11

To simplify the engineering efforts of implementing the PEP-II lattices, many modifications have been made to these lattices since the conceptual design report. During the development and evolution of the lattices, changes in a lattice would often result in a significant reduction in the dynamic aperture. At such times, we often relied on a non-linear analysis using the one-turn resonance basis Lie generator to identify the cause of the degradation. In this paper, we will present such examples to facilitate the usage of map for diagnosing the problems in lattice design.

Study of coupled-bunch collective effects in the PEP-II B-Factory

International Nuclear Information System (INIS)

Byrd, J.

1993-05-01

We present an overview of the calculated longitudinal and transverse coupled-bunch (CB) growth using the measured RF cavity higher order mode impedance and estimated resistive wall (RW) impedance for the proposed PEP-II B-Factory, a dual-ring electron-positron collider. We also describe a visual method of representing the effective beam impedance and corresponding growth rates which is especially useful for understanding the dependence of growth rate on higher order mode frequency and Q, spread of HOM frequencies between cells, and for determining the requirements of the CB feedback systems

Prodrugs of purine and pyrimidine analogues for the intestinal di/tri-peptide transporter PepT1

DEFF Research Database (Denmark)

Thomsen, Anne Engelbrecht; Friedrichsen, Gerda Marie; Sørensen, Arne Hagsten

2003-01-01

, novel L-Glu-Sar and D-Glu-Ala ester prodrugs of acyclovir and 1-(2-hydroxyethyl)-linked thymine were synthesized and their affinities for hPepT1 in Caco-2 cells were determined. Furthermore, the degradation of the prodrugs was investigated in various aqueous and biological media and compared...... to the corresponding hydrolysis of the prodrug valaciclovir. Affinity studies showed that the L-Glu-Sar prodrugs had high affinity for hPepT1 (K(i) approximately 0.2-0.3 mM), whereas the D-Glu-Ala prodrugs had poor affinity (K(i) approximately 50 mM). The pH-rate profiles of the prodrugs D-Glu[1-(2-hydroxyethyl......)thymine]-Ala and L-Glu[acyclovir]-Sar showed specific base catalyzed degradation at pH above 4.5 and 5.5, respectively. This implicates that the degradation rates at pH approximately 7.4 (t(1/2) approximately 3.5 and 5.5 h) are approximately 25 times faster than at upper small intestinal pH approximately 6.0. In 10...

SpirPep: an in silico digestion-based platform to assist bioactive peptides discovery from a genome-wide database.

Science.gov (United States)

Anekthanakul, Krittima; Hongsthong, Apiradee; Senachak, Jittisak; Ruengjitchatchawalya, Marasri

2018-04-20

Bioactive peptides, including biological sources-derived peptides with different biological activities, are protein fragments that influence the functions or conditions of organisms, in particular humans and animals. Conventional methods of identifying bioactive peptides are time-consuming and costly. To quicken the processes, several bioinformatics tools are recently used to facilitate screening of the potential peptides prior their activity assessment in vitro and/or in vivo. In this study, we developed an efficient computational method, SpirPep, which offers many advantages over the currently available tools. The SpirPep web application tool is a one-stop analysis and visualization facility to assist bioactive peptide discovery. The tool is equipped with 15 customized enzymes and 1-3 miscleavage options, which allows in silico digestion of protein sequences encoded by protein-coding genes from single, multiple, or genome-wide scaling, and then directly classifies the peptides by bioactivity using an in-house database that contains bioactive peptides collected from 13 public databases. With this tool, the resulting peptides are categorized by each selected enzyme, and shown in a tabular format where the peptide sequences can be tracked back to their original proteins. The developed tool and webpages are coded in PHP and HTML with CSS/JavaScript. Moreover, the tool allows protein-peptide alignment visualization by Generic Genome Browser (GBrowse) to display the region and details of the proteins and peptides within each parameter, while considering digestion design for the desirable bioactivity. SpirPep is efficient; it takes less than 20 min to digest 3000 proteins (751,860 amino acids) with 15 enzymes and three miscleavages for each enzyme, and only a few seconds for single enzyme digestion. Obviously, the tool identified more bioactive peptides than that of the benchmarked tool; an example of validated pentapeptide (FLPIL) from LC-MS/MS was demonstrated. The

[The analytical reliability of clinical laboratory information and role of the standards in its support].

Science.gov (United States)

Men'shikov, V V

2012-12-01

The article deals with the factors impacting the reliability of clinical laboratory information. The differences of qualities of laboratory analysis tools produced by various manufacturers are discussed. These characteristics are the causes of discrepancy of the results of laboratory analyses of the same analite. The role of the reference system in supporting the comparability of laboratory analysis results is demonstrated. The project of national standard is presented to regulate the requirements to standards and calibrators for analysis of qualitative and non-metrical characteristics of components of biomaterials.

A test of planar spark counters at the PEP storage ring

International Nuclear Information System (INIS)

Atwood, W.B.; Bowden, G.B.; Bonneaud, G.R.; Klem, D.E.; Ogawa, A.; Pestov, Yu.N.; Pitthan, R.; Sugahara, R.

1983-01-01

A test of planar spar counters (PSCs) at the PEP electron-positron storage ring showed the following. PSCs can be used under harsh experimental conditions without long term degradation of resolution. On-line time-of-flight resolutions were below 200 ps, coordinate resolutions better than 4 mm, both limited to these values by the resistivity of the semiconducting glass and the electronics used. The best single counter time resolution under realistic conditions at the storage ring was 76 ps. Pulse height was a good indicator of the number of particles striking a counter. Subsequent measurements using cosmis rays gave a single counter time resolution of 50 ps. (orig.)

High-power RF window design for the PEP-II B Factory

International Nuclear Information System (INIS)

Neubauer, M.; Hodgson, J.; Ng, C.; Schwarz, H.; Skarpaas, K.; Kroll, N.; Rimmer, R.

1994-06-01

We describe the design of RF windows to transmit up to 500 kW CW to the PEP-II 476 MHz cavities. RF analysis of the windows using high-frequency simulation codes are described. These provide information about the power loss distribution in the ceramic and tim matching properties of the structure. Finite-element analyses of the resulting temperature distribution and thermal stresses are presented. Fabrication methods including a proposed scheme to compensate for thermal expansion s are discussed and hardware tests to validate this approach are described. The effects of surface coatings (intentional and otherwise) and the application of air cooling are considered

Computational tools and lattice design for the PEP-II B-Factory

International Nuclear Information System (INIS)

Cai, Y.; Irwin, J.; Nosochkov, Y.; Yan, Y.

1997-01-01

Several accelerator codes were used to design the PEP-II lattices, ranging from matrix-based codes, such as MAD and DIMAD, to symplectic-integrator codes, such as TRACY and DESPOT. In addition to element-by-element tracking, we constructed maps to determine aberration strengths. Furthermore, we have developed a fast and reliable method (nPB tracking) to track particles with a one-turn map. This new technique allows us to evaluate performance of the lattices on the entire tune-plane. Recently, we designed and implemented an object-oriented code in C++ called LEGO which integrates and expands upon TRACY and DESPOT. copyright 1997 American Institute of Physics

Pizza Parties, Pep Rallies, and Practice Tests: Strategies Used by High School Principals to Raise Percent Proficient

Science.gov (United States)

Hollingworth, Liz; Dude, David J.; Shepherd, Julie K.

2010-01-01

This study explores ways high school principals are responding to the demands of education reform to raise student test scores on achievement tests used for accountability purposes. Anecdotal evidence suggests administrators have instituted pizza parties and pep rallies to motivate students to do their best and practice tests to prepare students…

Analyses in support of the Laboratory Microfusion Facility and ICF commercial reactor designs

International Nuclear Information System (INIS)

Meier, W.R.; Monsler, M.J.

1988-01-01

Our work on this contract was divided into two major categories; two thirds of the total effort was in support of the Laboratory Microfusion Facility (LMF), and one third of the effort was in support of Inertial Confinement Fusion (ICF) commercial reactors. This final report includes copies of the formal reports, memoranda, and viewgraph presentations that were completed under this contract

Stanford Synchrotron Radiation Laboratory activity report for 1986

Energy Technology Data Exchange (ETDEWEB)

Cantwell, K. [ed.

1987-12-31

1986 was another year of major advances for SSRL as the ultimate capabilities of PEP as a synchrotron radiation source became more apparent and a second PEP beam line was initiated, while effective development and utilization of SPEAR proceeded. Given these various PEP developments, SSRL abandoned its plans for a separate diffraction limited ring, as they abandoned their plans for a 6--7 GeV ring of the APS type last year. It has become increasingly apparent that SSRL should concentrate on developing SPEAR and PEP as synchrotron radiation sources. Consequently, initial planning for a 3 GeV booster synchrotron injector for SPEAR was performed in 1986, with a proposal to the Department of Energy resulting. As described in Chapter 2, the New Rings Group and the Machine Physics Group were combined into one Accelerator Physics Group. This group is focusing mainly on the improvement of SPEAR`s operating conditions and on planning for the conversion of PEP into a fourth generation x-ray source. Considerable emphasis is also being given to the training of accelerator physics graduate students. At the same time, several improvements of SSRL`s existing facilities were made. These are described in Chapter 3. Chapter 4 describes new SSRL beam lines being commissioned. Chapter 5 discusses SSRL`s present construction projects. Chapter 6 discusses a number of projects presently underway in the engineering division. Chapter 7 describes SSRL`s advisory panels while Chapter 8 discusses SSRL`s overall organization. Chapter 9 describes the experimental progress reports.

Reação de híbridos, linhagens e progênies de pimentão à requeima causada por Phytophthora capsici e ao mosaico amarelo causado por Pepper yellow mosaic vírus (PepYMV Reaction of hybrids, lines and progenies of sweet pepper the blight caused by Phytophthora capsici and to Pepper yellow mosaic virus (PepYMV

Directory of Open Access Journals (Sweden)

Ildon Rodrigues do Nascimento

2007-02-01

Full Text Available A requeima do pimentão (Capsicum annuum L. causada por Phytophthora capsici e o mosaico amarelo causado por PepYMV são prioridades nos programas de melhoramento de pimentão em andamento no Brasil. Foram avaliados três híbridos comerciais (Magali R, Atenas F1 e Fortuna Super F1, cinco linhagens, 20 híbridos experimentais e duas progênies F2:4 do programa de melhoramento da Universidade Federal de Lavras/HortiAgro Sementes quanto à reação a P. capsici e a PepYMV. Os experimentos foram montados independentemente em blocos casualizados, com quatro e duas repetições, respectivamente. Cada parcela foi constituída de oito plantas instaladas em bandejas de 128 células, que foram mantidas em estufa com cobertura plástica e laterais teladas. As avaliações foram feitas do 4º ao 14º e do 15º ao 40º dias após a inoculação (DAI para P. capsici e PepYMV, respectivamente. A inoculação com P. capsici foi feita pormeio da distribuição de 5 mL de suspensão com 10(4 zoósporos/mL no solo ao redor do colo de cada planta. A inoculação mecânica de PepYMV foi feita a partir de macerados de folhas de Nicotiana tabacum cv. TNN, previamente infectadas. Os híbridos Magali R e Fortuna Super, foram suscetíveis a P. capsici, enquanto que o acesso Criollo de Morellos 334, a linhagem PIM-013 e as progênies PIX-03 pl#03-2 e PIX-030 pl#06-3 (ambas originadas do cruzamento com Criollo de Morellos, foram resistentes. Reação de resistência a P. capsici foi também observada para os híbridos experimentais que tiveram PIM-013 como uma das linhagens parentais. Criollo de Morellos 334, as progênies PIX-03 pl#03-2 e PIX-030 pl#06-3, o híbrido comercial Magali R e outros 6 híbridos experimentais que tinham como um dos genitores a linhagem MYR-29 forma ressitentes ao PepYMV. As progênies PIX-03 pl#03-2 e PIX-030 pl#06-3 são fontes promissoras de resistência a ambos os patógenos para serem desenvolvidas e exploradas em programas de melhoramento

Support of the Laboratory in the Diagnosis of Fungal Ocular Infections

Science.gov (United States)

Vanzzini Zago, Virginia; Alcantara Castro, Marino; Naranjo Tackman, Ramon

2012-01-01

This is a retrospective, and descriptive study about the support that the laboratory of microbiology aids can provide in the diagnosis of ocular infections in patients whom were attended a tertiary-care hospital in México City in a 10-year-time period. We describe the microbiological diagnosis in palpebral mycose; in keratitis caused by Fusarium, Aspergillus, Candida, and melanized fungi; endophthalmitis; one Histoplasma scleritis and one mucormycosis. Nowadays, ocular fungal infections are more often diagnosed, because there is more clinical suspicion and there are easy laboratory confirmations. Correct diagnosis is important because an early medical treatment gives a better prognosis for visual acuity. In some cases, fungal infections are misdiagnosed and the antifungal treatment is delayed. PMID:22518339

PEP conceptual design report

International Nuclear Information System (INIS)

1976-02-01

The accelerator system design, the physical plant, the experimental areas, cost estimates, and schedules for PEP are discussed. The main component of the proposed facility is a storage ring in which beams of positrons and electrons circulate in opposite directions in a vacuum chamber embedded in a magnetic guide field having six bending arcs and six long straight sections. The electrons and positrons to be stored in it are produced in the SLAC linac and are introduced into the storage ring via two beam transport paths emanating from the end of the two-mile accelerator. Beams of energies up to 18 GeV can be stored, and, at a future date, components could be added to permit energies as high as 22 GeV. Provisions are also made in the design of the ring housing so that a synchrotron-radiation research facility could be added in the future. The energy lost from the beams by synchrotron radiation is restored by a high-power radio frequency accelerating system which employs klystrons to drive the accelerating structures at a frequency of 353 MHz. The system is capable of delivering five megawatts of power to the beams. Low pressures will be sustained by means of long, narrow sputter-ion pumps located in the vacuum chamber in the bending magnets directly alongside the beams. The proposed storage ring is designed to generate a luminosity (reaction rate per unit reaction cross section) of more than 10 31 cm -2 sec -1 per interaction region at beam energies between 5 GeV and 18 GeV and a maximum luminosity of 10 32 cm -2 sec -1 per interaction region at a beam energy of 15 GeV

Effectiveness of Podcasts Delivered on Mobile Devices as a Support for Student Learning during General Chemistry Laboratories

Science.gov (United States)

Powell, Cynthia B.; Mason, Diana S.

2013-01-01

Chemistry instructors in teaching laboratories provide expert modeling of techniques and cognitive processes and provide assistance to enrolled students that may be described as scaffolding interaction. Such student support is particularly essential in laboratories taught with an inquiry-based curriculum. In a teaching laboratory with a high…

Self-diffusion investigations on a series of PEP-PDMS diblock copolymers with different morphologies by pulsed field gradient NMR

DEFF Research Database (Denmark)

Rittig, F.; Karger, J.; Papadakis, C.M.

1999-01-01

We report on temperature-dependent self-diffusion measurements of compositionally different and non-entangled poly(ethylene-co-propylene)-b-poly(dimethylsiloxane) PEP-PDMS diblock copolymers in the melt above and below the order-to-disorder transition temperature. Depending on the dimensionality...

The vacuum system for the PEP II high energy ring straight sections

International Nuclear Information System (INIS)

Wienands, U.; Daly, E.; Heifets, S.A.; Kulikov, A.; Kurita, N.; Nordby, M.; Perkins, C.; Reuter, E.; Seeman, J.T.; Belser, F.C.; Berg, J.; Holdener, F.R.; Kerns, J.A.; McDaniel, M.R.; Stoeffl, W.

1995-01-01

The six straight sections of the PEP II High Energy Ring (HER) serve various functions: lattice tuning, beam injection and abort, providing space for rf cavities, longitudinal and transverse feedback, beam diagnostics and the interaction point. A stainless steel vacuum system has been designed; prototypes are currently being built. Cooling is required due to radiation coming from the last arc dipole and resistive losses in the vacuum chamber. Although the nominal beam current of the HER is 1 A the vacuum system is designed for 3 A to provide margin and an upgrade path. 5 refs., 7 figs

Effects of parasitic beam-beam interaction during the injection process at the PEP-II B Factory

International Nuclear Information System (INIS)

Chin, Y.H.

1992-06-01

This paper is concerned with beam-beam effects during the injection process at the proposed asymmetric SLAC/LBL/LLNL B-Factory, PEP-II. It is shown that the parasitic beam-beam interaction can lead to a significant blowup in the vertical size of the injected beam. Simulation results for the horizontal and the vertical injection schemes are presented, and their performances are studied

Harmonic strengths of PEP dipoles and some related effects and lessons

International Nuclear Information System (INIS)

Spencer, J.E.

1981-09-01

The harmonic content of magnets such as the standard PEP bend is (among other things) a function of excitation current, the way the current is set and even the magnetization history. For instance, harmonic strengths generally vary not only with the magnitude of the current but the direction and rate at which the current is approached and set. The field distribution resulting from different procedures can vary markedly depending on both the mechanical and magnetic design and the degree to which eddy current effects are emphasized. Variations among magnets of the same design result from variations in the iron as well as overall magnet fabrication procedures. Because the field distribution may also depend in the previous history of a magnet, all PEP dipoles were subjected to what are called ''magnetization'' and ''standardization'' cycles before measurement---the latter depending on the former and intended to set the initial conditions of the magnet to a reproducible standard. The primary goal of the magnetic measurements was then to determine the dipole strength as a function of current for each magnet based on a practical setting algorithm. The main constraints on the algorithm were reproducibility of the integrated field, speed, power and reduction of higher harmonics. Quadrupole and sextupole strengths were also measured on about one-half of the magnets at one current. This note presents the data and discusses it from the the viewpoint of subsequent measurements with stored beams. The most important conclusion is that inability to fully distribute laminations according to heat number and/or strike number results in ''magnetic personalities'' among the magnets which are quite difficult to deal with afterwards although one can distribute ''non-standard'' magnets to minimize orbit distributions. 26 refs., 8 figs., 3 tabs

miR-378 Activates the Pyruvate-PEP Futile Cycle and Enhances Lipolysis to Ameliorate Obesity in Mice

Directory of Open Access Journals (Sweden)

Yong Zhang

2016-03-01

Full Text Available Obesity has been linked to many health problems, such as diabetes. However, there is no drug that effectively treats obesity. Here, we reveal that miR-378 transgenic mice display reduced fat mass, enhanced lipolysis, and increased energy expenditure. Notably, administering AgomiR-378 prevents and ameliorates obesity in mice. We also found that the energy deficiency seen in miR-378 transgenic mice was due to impaired glucose metabolism. This impairment was caused by an activated pyruvate-PEP futile cycle via the miR-378-Akt1-FoxO1-PEPCK pathway in skeletal muscle and enhanced lipolysis in adipose tissues mediated by miR-378-SCD1. Our findings demonstrate that activating the pyruvate-PEP futile cycle in skeletal muscle is the primary cause of elevated lipolysis in adipose tissues of miR-378 transgenic mice, and it helps orchestrate the crosstalk between muscle and fat to control energy homeostasis in mice. Thus, miR-378 may serve as a promising agent for preventing and treating obesity in humans.

Design of a high-power test model of the PEP-II rf cavity

International Nuclear Information System (INIS)

Schwarz, H.D.; Bell, R.A.; Hodgson, J.A.

1993-05-01

The design of a normal-conducting high-power test cavity (HPTC) for PEP-II is described. The cavity includes HOM loading waveguides and provisions for testing two alternate input coupling schemes. 3-D electromagnetic field simulations provided input information for the surface power deposition. Finite element codes were utilized for thermal and stress analyses of the cavity to arrive at a suitable mechanical design capable of handling the high power dissipation. The mechanical design approach with emphasis on the cooling channel layout and mechanical stress reduction is described

In roots of Arabidopsis thaliana, the damage-associated molecular pattern AtPep1 is a stronger elicitor of immune signalling than flg22 or the chitin heptamer.

Directory of Open Access Journals (Sweden)

Lorenzo Poncini

Full Text Available Plants interpret their immediate environment through perception of small molecules. Microbe-associated molecular patterns (MAMPs such as flagellin and chitin are likely to be more abundant in the rhizosphere than plant-derived damage-associated molecular patterns (DAMPs. We investigated how the Arabidopsis thaliana root interprets MAMPs and DAMPs as danger signals. We monitored root development during exposure to increasing concentrations of the MAMPs flg22 and the chitin heptamer as well as of the DAMP AtPep1. The tissue-specific expression of defence-related genes in roots was analysed using a toolkit of promoter::YFPN lines reporting jasmonic acid (JA-, salicylic acid (SA-, ethylene (ET- and reactive oxygen species (ROS- dependent signalling. Finally, marker responses were analysed during invasion by the root pathogen Fusarium oxysporum. The DAMP AtPep1 triggered a stronger activation of the defence markers compared to flg22 and the chitin heptamer. In contrast to the tested MAMPs, AtPep1 induced SA- and JA-signalling markers in the root and caused a severe inhibition of root growth. Fungal attack resulted in a strong activation of defence genes in tissues close to the invading fungal hyphae. The results collectively suggest that AtPep1 presents a stronger danger signal to the Arabidopsis root than the MAMPs flg22 and chitin heptamer.

Dipeptide model prodrugs for the intestinal oligopeptide transporter. Affinity for and transport via hPepT1 in the human intestinal Caco-2 cell line

DEFF Research Database (Denmark)

Nielsen, C U; Andersen, R; Brodin, Birger

2001-01-01

-moieties for benzyl alcohol have been shown to maintain affinity for hPepT1. The primary aim of the present study was to investigate if modifications of the benzyl alcohol model drug influence the corresponding D-Glu-Ala and D-Asp-Ala model prodrugs' affinity for hPepT1 in Caco-2 cells. A second aim...... was to investigate the transepithelial transport and hydrolysis parameters for D-Asp(BnO)-Ala and D-Glu(BnO)-Ala across Caco-2 cell monolayers. In the present study, all investigated D-Asp-Ala and D-Glu-Ala model prodrugs retained various degrees of affinity for hPepT1 in Caco-2 cells. These affinities are used....... Transepithelial transport studies performed using Caco-2 cells of D-Asp(BnO)-Ala and D-Glu(BnO)-Ala showed that the K(m) for transepithelial transport was not significantly different for the two compounds. The maximal transport rate of the carrier-mediated flux component does not differ between the two model...

Streak-Camera Measurements with High Currents in PEP-II and Variable Optics in SPEAR3

Energy Technology Data Exchange (ETDEWEB)

Cheng, Weixeng; Fisher, Alan, a Corbett, Jeff; /SLAC

2008-06-05

A dual-axis, synchroscan streak camera was used to measure longitudinal bunch profiles in three storage rings at SLAC: the PEP-II low- and high-energy rings, and SPEAR3. At high currents, both PEP rings exhibit a transient synchronous-phase shift along the bunch train due to RF-cavity beam loading. Bunch length and profile asymmetry were measured along the train for a range of beam currents. To avoid the noise inherent in a dual-axis sweep, we accumulated single-axis synchroscan images while applying a 50-ns gate to the microchannel plate. To improve the extinction ratio, an upstream mirror pivoting at 1 kHz was synchronized with the 2kHz MCP gate to deflect light from other bunches off the photocathode. Bunch length was also measured on the HER as a function of beam energy. For SPEAR3 we measured bunch length as a function of single-bunch current for several lattices: achromatic, low-emittance and low momentum compaction. In the first two cases, resistive and reactive impedance components can be extracted from the longitudinal bunch profiles. In the low-alpha configurations, we observed natural bunch lengths approaching the camera resolution, requiring special care to remove instrumental effects, and saw evidence of periodic bursting.

Identifying Lattice, Orbit, And BPM Errors in PEP-II

International Nuclear Information System (INIS)

Decker, F.-J.; SLAC

2005-01-01

The PEP-II B-Factory is delivering peak luminosities of up to 9.2 · 10 33 1/cm 2 · l/s. This is very impressive especially considering our poor understanding of the lattice, absolute orbit and beam position monitor system (BPM). A few simple MATLAB programs were written to get lattice information, like betatron functions in a coupled machine (four all together) and the two dispersions, from the current machine and compare it the design. Big orbit deviations in the Low Energy Ring (LER) could be explained not by bad BPMs (only 3), but by many strong correctors (one corrector to fix four BPMs on average). Additionally these programs helped to uncover a sign error in the third order correction of the BPM system. Further analysis of the current information of the BPMs (sum of all buttons) indicates that there might be still more problematic BPMs

HOM power propagation and attenuation in PEP-II B-factory

International Nuclear Information System (INIS)

Lin, X.E.; Ng, C.K.; Ko, K.

1997-09-01

Most of the higher-order-mode (HOM) power that propagates in the PEP-II rings is generated i the RF cavities but its content in terms of TE and TM components has not been accurately determined. For purpose of shielding beamline components such as bellows and BPMs from TE power penetration and determining the heating on the cavity HOM absorber, this HOM power content and its distribution profile around the rings are needed. This paper calculates the TE and TM contributions of the RF cavities to the circulating HOM power and their transmission properties at another cavity downstream. By considering both the generation in, and scattering by the cavities, as well as the attenuation along the vacuum chamber, a complete distribution profile for the TE and TM HOM power can be obtained

NucLab Marcoule. A laboratory facility dedicated to support dismantling operations

International Nuclear Information System (INIS)

Dugne, O.; Houssin, A.; Pierre, D.; Bec-Espitalier, L.

2013-06-01

Formerly dedicated to plutonium production support, NucLab was renovated to perform a wide range of analyses for dismantling, plant operation and process development activities mainly on Marcoule site but also outside (Veurey, Fontenay aux Roses). The Laboratory is under a CEA AREVA partnership as a CEA entity operated by AREVA employees. It provides services to several industrial operators (nuclear process and power plant) in the fields of analytical chemistry, radioactivity measurements, in situ nuclear measurements, decontamination processes and industrial chemistry processes, waste treatments to meet the following analysis requirements. NucLab today is able to support research, production and dismantling activities in all part of dismantling operations. (authors)

Improvement of PEP-II Linear Optics with a MIA-Derived Virtual Accelerator

International Nuclear Information System (INIS)

Cerio, B.; Colgate U.

2006-01-01

In several past studies, model independent analysis, in conjunction with a virtual accelerator model, has been successful in improving PEP-II linear geometric optics. In many cases, optics improvement yielded an increase in machine luminosity. In this study, an updated characterization of linear optics is presented. With the PEP-II beam position monitor (BPM) system, four independent beam centroid orbits were extracted and used to determine phase advances and linear Green's functions among BPM locations. A magnetic lattice model was then constructed with a singular value decomposition-enhanced least-square fitting of phase advances and Green's functions, which are functions of quadrupole strengths, sextupole feed-downs, as well as BPM errors, to the corresponding measured quantities. The fitting process yielded a machine model that matched the measured linear optics of the real machine and was therefore deemed the virtual accelerator. High beta beat, as well as linear coupling, was observed in both LER and HER of the virtual accelerator. Since there was higher beta beating in LER, focus was shifted to the improvement of this ring. By adjusting select quadrupoles of the virtual LER and fitting the resulting beta functions and phase advances to those of the desired lattice, the average beta beat of the virtual machine was effectively reduced. The new magnet configuration was dialed into LER on August 10, 2006, and beta beat was reduced by a factor of three. After fine tuning HER to match the improved LER for optimal collision, a record peak luminosity of 12.069 x 10 33 cm -2 s -1 was attained on August 16, 2006

Progress on PEP-II injection R ampersand D

International Nuclear Information System (INIS)

Bloom, E.; Bulos, F.; Fieguth, T.; Godfrey, G.; Loew, G.; Miller, R.

1993-06-01

The R ampersand D program described in this paper focuses on an improvement of the SLAC linac designed to extract and study a 9 GeV electron beam under stringent control of energy, energy spread, emittance, optical parameters, and timing. The extraction system begins with an on-axis pulsed magnet, followed by a magnetic lattice and diagnostic equipment required for the measurement and optimization of the above beam qualities. Design, construction, and installation of this system is the first step in the development of the overall PEP-II e ± injection system. This system is required to fill 1658 bunches of 9 GeV electrons (0.99A stored) and 3.1 GeV positrons (2.14A stored) in two separate rings in a total of about 6 minutes from zero ring current (i.e., full-fill mode, 0 to 100%) or in about 3 minutes from 80% ring current (i.e., topping-off mode, 80 to 100%). This unprecedented rate of filling can be met by a judicious use of the SLC linac, damping rings, and positron source

Progress on PEP-II injection R ampersand D

International Nuclear Information System (INIS)

Bloom, E.; Bulos, F.; Fieguth, T.; Godfrey, G.; Loew, G.; Miller, R.

1993-01-01

The R ampersand D program described in this paper focuses on an improvement of the SLAC linac designed to extract and study a 9 GeV electron beam under stringent control of energy, energy spread, emittance, optical parameters, and timing. The extraction system begins with an on-axis pulsed magnet, followed by a magnetic lattice and diagnostic equipment required for the measurement and optimization of the above beam qualities. Design, construction, and installation of this system is the first step in the development of the overall PEP-II e ± injection system. This system is required to fill 1658 bunches of 9 GeV electrons (0.99A stored) and 3.1 GeV positrons (2.14A stored) in two separate rings in a total of about 6 minutes from zero ring current (i.e., full-fill mode, 0 to 100%) or in about 3 minutes from 80% ring current (i.e., topping-off mode, 80 to 100%). This unprecedented rate of filling can be met by a judicious use of the SLC linac, damping rings, and positron source

Design of traveling wave windows for the PEP-II RF coupling network

International Nuclear Information System (INIS)

Kroll, N.M.; Ng, C.K.; Judkins, J.; Neubauer, M.

1995-05-01

The waveguide windows in the PEP-II RF coupling network have to withstand high power of 500 kW. Traveling wave windows have lower power dissipation than conventional self-matched windows, thus rendering the possibility of less stringent mechanical design. The traveling wave behavior is achieved by providing a reflecting iris on each side of the window, and depending on the configuration of the irises, traveling wave windows are characterized as inductive or capacitive types. A numerical design procedure using MAFIA has been developed for traveling wave windows. The relative advantages of inductive and capacitive windows are discussed. Furthermore, the issues of bandwidth and multipactoring are also addressed

Secondary Electron Emission Yields from PEP-II Accelerator Materials

International Nuclear Information System (INIS)

Kirby, Robert E.

2000-01-01

The PEP-II B-Factory at SLAC operates with aluminum alloy and copper vacuum chambers, having design positron and electron beam currents of 2 and 1 A, respectively. Titanium nitride coating of the aluminum vacuum chamber in the arcs of the positron ring is needed in order to reduce undesirable electron-cloud effects. The total secondary electron emission yield of TiN-coated aluminum alloy has been measured after samples of beam chamber material were exposed to air and again after electron-beam bombardment, as a function of incident electron beam angle and energy. The results may be used to simulate and better understand electron-cloud effects under actual operating conditions. We also present yield measurements for other accelerator materials because new surface effects are expected to arise as beam currents increase. Copper, in particular, is growing in popularity for its good thermal conductivity and self-radiation-shielding properties. The effect of electron bombardment, ''conditioning'', on the yield of TiN and copper is shown

Sensitivity of the PEP beam transport line to perturbations

International Nuclear Information System (INIS)

Peterson, J.M.; Brown, K.L.

1979-03-01

The sensitivity of a beam-transport line to various perturbations determines the extent to which one can simplify component design and relax tolerances. For the PEP injection lines, effects of various fabrication errors, magnet misalignments, and residual gas scattering were studied. Using the TURTLE ray-tracing program, it is found that magnetic-field errors corresponding to a relative sextupole strength in the dipoles of 0.5% and/or a relative sextupole or octupole strength in the quadrupoles of 5% are permissible. This allows relatively loose tolerances in magnet fabrication. Transverse misalignment of a quadrupole by a distance x causes the beam centroid to be displaced downstream by as much as 5x. This requires a quadrupole alignment accuracy of +- 0.5 mm or better. No compensation for the earth's field is necessary because an integral number of optical wavelengths and a short wavelength were used for the design. Analysis shows that beam broadening from multiple coulomb scattering is insignificant for pressures of less than 1/10 torr

Higher order mode damping studies on the PEP-II B-Factory RF cavity

International Nuclear Information System (INIS)

Rimmer, R.; Goldberg, D.; Lambertson, G.; Voelker, F.; Ko, K.; Kroll, N.; Pendleton, R.; Schwarz, H.; Adams, F.; De Jong, M.

1992-03-01

We describe studies of the higher-order-mode (HOM) properties of the prototype 476 MHz RF cavity for the proposed PEP-II B-Factory and a waveguide damping scheme to reduce possible HOM-driven coupled-bunch beam instability growth. Numerical studies include modelling of the HOM spectrum using MAFIA and ARGUS, and calculation of the loaded Q's of the damped modes using data from these codes and the Kroll-Yu method. We discuss briefly the experimental investigations of the modes, which will be made in a full-size low-power test cavity, using probes, wire excitation and bead perturbation methods

High-power RF cavity R ampersand D for the PEP-II B Factory

International Nuclear Information System (INIS)

Rimmer, R.; Lambertson, G.; Hodgson, J.

1994-06-01

We describe the development of a high-power test model of the 476 MHz RF cavity for the PEP-II B Factory. This cavity is designed to demonstrate the feasibility of a high-power design with higher-order mode (HOM) damping waveguides and the fabrication technologies involved, and it can also be used to evaluate aperture or loop couplers and various RF windows. Changes to the RF design to reduce peak surface heating are discussed and results of finite-element analyses of temperature and stress are presented. Fabrication methods for the prototype and subsequent production cavities are discussed

Developing Novel PepT1-Targeted Modulators for Inflammatory Bowel Disease (IBD) Therapy

Science.gov (United States)

2016-10-01

physiological roles. PepT1 is found in the small intestine where it absorbs dietary degradation products and therapeutic agents such as antibiotics ...results provide a framework for developing more potent ligands in various cellular and animal IBD models, directly addressing Aims 2 and 3 of our... Products …………………………………….………….……………4 7. Participants & Other Collaborating Organizations…….…………….5 8. Special Reporting Requirements…………..………………………5 9

Several hPepT1-transported drugs are substrates of the Escherichia coli proton-coupled oligopeptide transporter YdgR

DEFF Research Database (Denmark)

Prabhala, Bala K; Aduri, Nanda G; Iqbal, Mazhar

2017-01-01

transported by hPepT1. The transport of these drugs was evaluated using the prototypical POT YdgR from E. coli. The transport studies were pursued through combining cell-based assays with liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. These investigations revealed that YdgR from E. coli...

Quality assurance guidance for laboratory assessment plates in support of EM environmental sampling and analysis activities

International Nuclear Information System (INIS)

1994-05-01

This document is one of several guidance documents developed to support the EM (DOE Environmental Restoration and Waste Management) Analytical Services program. Its purpose is to introduce assessment plates that can be used to conduct performance assessments of an organization's or project's ability to meet quality goals for analytical laboratory activities. These assessment plates are provided as non-prescriptive guidance to EM-support organizations responsible for collection of environmental data for remediation and waste management programs at DOE facilities. The assessments evaluate objectively all components of the analytical laboratory process to determine their proper selection and use

Laboratory interface in support of Environmental Restoration Programs

International Nuclear Information System (INIS)

Pardue, G.J. Jr.

1994-01-01

A vital part of quality environmental data resides in the communication between the project and the analytical laboratory. It is essential that the project clearly identify its objectives to the laboratory and that the laboratory understands the scope and limitations of the analytical process. Successful completion of an environmental project must include an aggressive program between project managers and subcontracted Lyrical laboratories. All to often, individuals and organizations tend to deflect errors and failures observed in environmental toward open-quotes the other guyclose quotes. The engineering firm will blame the laboratory, the laboratory will blame the field operation, the field operation will blame the engineering, and everyone will blame the customer for not understanding the true variables in the environmental arena. It is the contention of the authors, that the majority of failures derive from a lack of communication and misunderstanding. Several initiatives can be taken to improve communication and understanding between the various pieces of the environmental data quality puzzle. This presentation attempts to outline mechanisms to improve communication between the environmental project and the analytical laboratory with the intent of continuous quality improvement. Concepts include: project specific laboratory statements of work which focus on project and program requirements; project specific analytical laboratory readiness reviews (project kick-off meetings); laboratory team workshops; project/program performance tracking and self assessment and promotion of team success

Roles of the International Council for Laboratory Animal Science (ICLAS) and International Association of Colleges of Laboratory Animal Medicine (IACLAM) in the Global Organization and Support of 3Rs Advances in Laboratory Animal Science

Science.gov (United States)

Turner, Patricia V; Pekow, Cynthia; Clark, Judy MacArthur; Vergara, Patri; Bayne, Kathryn; White, William J; Kurosawa, Tsutomu Miki; Seok, Seung-Hyeok; Baneux, Philippe

2015-01-01

Practical implementation of the 3Rs at national and regional levels around the world requires long-term commitment, backing, and coordinated efforts by international associations for laboratory animal medicine and science, including the International Association of Colleges of Laboratory Animal Medicine (IACLAM) and the International Council for Laboratory Animal Science (ICLAS). Together these organizations support the efforts of regional organization and communities of laboratory animal science professionals as well as the development of local associations and professional colleges that promote the training and continuing education of research facility personnel and veterinary specialists. The recent formation of a World Organization for Animal Health (OIE) Collaborating Center for Laboratory Animal Science and Welfare emphasizes the need for research into initiatives promoting laboratory animal welfare, particularly in emerging economies and regions with nascent associations of laboratory animal science. PMID:25836964

Performance of health laboratories in provision of HIV diagnostic and supportive services in selected districts of Tanzania.

Science.gov (United States)

Ishengoma, Deus S; Kamugisha, Mathias L; Rutta, Acleus S M; Kagaruki, Gibson B; Kilale, Andrew M; Kahwa, Amos; Kamugisha, Erasmus; Baraka, Vito; Mandara, Celine I; Materu, Godlisten S; Massaga, Julius J; Magesa, Stephen M; Lemnge, Martha M; Mboera, Leonard E G

2017-01-23

Roll-out and implementation of antiretroviral therapy (ART) necessitated many countries in Sub-Saharan Africa to strengthen their national health laboratory systems (NHLSs) to provide high quality HIV diagnostic and supportive services. This study was conducted to assess the performance of health laboratories in provision of HIV diagnostic and supportive services in eight districts (from four regions of Iringa, Mtwara, Tabora and Tanga), after nine years of implementation of HIV/AIDS care and treatment plan in Tanzania. In this cross-sectional study, checklists and observations were utilized to collect information from health facilities (HFs) with care and treatment centres (CTCs) for HIV/AIDS patients; on availability of laboratories, CTCs, laboratory personnel, equipment and reagents. A checklist was also used to collect information on implementation of quality assurance (QA) systems at all levels of the NHLS in the study areas. The four regions had 354 HFs (13 hospitals, 41 Health Centres (HCs) and 300 dispensaries); whereby all hospitals had laboratories and 11 had CTCs while 97.5 and 61.0% of HCs had both laboratories and CTCs, respectively. Of the dispensaries, 36.0 and 15.0% had laboratories and CTCs (mainly in urban areas). Thirty nine HFs (12 hospitals, 21 HCs and six dispensaries) were assessed and 56.4% were located in urban areas. The assessed HFs had 199 laboratory staff of different cadres (laboratory assistants = 35.7%; technicians =32.7%; attendants = 22.6%; and others = 9.1%); with >61% of the staff and 72.3% of the technicians working in urban areas. All laboratories were using rapid diagnostic tests for HIV testing. Over 74% of the laboratories were performing internal quality control and 51.4% were participating in external QA programmes. Regional and district laboratories had all key equipment and harmonization was maintained for Fluorescence-Activated Cell Sorting (FACS) machines. Most of the biochemical (58.0%) and haematological

Beam-beam dynamics during the injection process at the PEP-II B-Factory

International Nuclear Information System (INIS)

Chin, Yong Ho.

1991-10-01

This paper is concerned with beam-beam effects during the injection process at the proposed asymmetric SLAC/LBL/LLNL B-Factory based on PEP (PEP-2). For symmetric colliders, the primary source of the beam-beam effect is the head-on collision at the interaction point (IP), and this effect can be mitigated by separating the beams during the injection process. For an asymmetric collider, which intrinsically consists of two separate rings, the bunches not only collide at the IP but experience a long-range beam-beam force on the way into and out of the IP region. These collisions are called ''parasitic crossings (PC).'' The parasitic crossings emerge as a potential source of far stronger beam-beam impact during the injection process for the following reason. In the proposed injection scheme of the APIARY-6.3d design, the bunches are injected horizontally into the two rings with large horizontal offset of 8σ Ox sptm where σ Ox sptm is the nominal horizontal storage ring beam size at the end of the septum magnet. Then, the injected beam starts to travel around the ring oscillating horizontally. For the sake of discussion, let us assume that the beam in the other ring has already been fully stored. When the injected beam arrives at the 1st PC, where the two nominal orbits are separated horizontally by about 7.6 times the nominal horizontal beam size of the low energy ring, it may pass through the other beam far more closely than at the nominal separation distance, or it may even strike the other beam head-on

Low energy ring lattice of the PEP-II asymmetric B-Factory

International Nuclear Information System (INIS)

Cai, Y.; Donald, M.; Helm, R.; Irwin, J.; Nosochkov, Y.; Ritson, D.M.; Yan, Y.

1995-01-01

Developing a lattice that contains a very low beta value at the interaction point (IP) and has adequate dynamic aperture is one of the major challenges in designing the PEP-II asymmetric B-factory. For the Low Energy Ring (LER) the authors have studied several different chromatic correction schemes since the conceptual design report (CDR). Based on these studies, a hybrid solution with local and semi-local chromatic sextupoles has been selected as the new baseline lattice to replace the local scheme in the CDR. The new design simplifies the interaction region (IR) and reduces the number of sextupoles in the arcs. Arc sextupoles are paired at π phase difference and are not interleaved. In this paper the authors describe the baseline lattice with the emphasis on the lattice changes made since the CDR

Role of the Microcomputer-Based Laboratory Display in Supporting the Construction of New Understandings in Thermal Physics

Science.gov (United States)

Russell, David W.; Lucas, Keith B.; McRobbie, Campbell J.

2004-01-01

Teachers' failure to use the microcomputer-based laboratory (MBL) more widely may be a result of not recognizing its capacity to transform laboratory activities. This research aimed to increase understanding of how MBL activities designed to be consistent with a constructivist theory of learning support or constrain student construction of…

A New Chicane Experiment In PEP-II to Test Mitigations of the Electron Cloud Effect for Linear Colliders

International Nuclear Information System (INIS)

Pivi, M

2008-01-01

Beam instability caused by the electron cloud has been observed in positron and proton storage rings, and it is expected to be a limiting factor in the performance of future colliders [1-3]. The effect is expected to be particularly severe in magnetic field regions. To test possible mitigation methods in magnetic fields, we have installed a new 4-dipole chicane experiment in the PEP-II Low Energy Ring (LER) at SLAC with both bare and TiN-coated aluminum chambers. In particular, we have observed a large variation of the electron flux at the chamber wall as a function of the chicane dipole field. We infer this is a new high order resonance effect where the energy gained by the electrons in the positron beam depends on the phase of the electron cyclotron motion with respect to the bunch crossing, leading to a modulation of the secondary electron production. Presumably the cloud density is modulated as well and this resonance effect could be used to reduce its magnitude in future colliders. We present the experimental results obtained during January 2008 until the April final shut-down of the PEP-II machine

Underground test of quantum mechanics - the VIP2 experiment arXiv

CERN Document Server

Marton, Johann; Bassi, A.; Bazzi, M.; Bertolucci, S.; Berucci, C.; Bragadireanu, M.; Cargnelli, M.; Clozza, A.; Curceanu, C.; De Paolis, L.; Di Matteo, S.; Donadi, S.; Egger, J.-P.; Guaraldo, C.; Iliescu, M.; Laubenstein, M.; Milotti, E.; Pichler, A.; Pietreanu, D.; Piscicchia, K.; Scordo, A.; Shi, H.; Sirghi, D.; Sirghi, F.; Sperandio, L.; Vazquez-Doce, O.; Widmann, E.; Zmeskal, J.

We are experimentally investigating possible violations of standard quantum mechanics predictions in the Gran Sasso underground laboratory in Italy. We test with high precision the Pauli Exclusion Principle and the collapse of the wave function (collapse models). We present our method of searching for possible small violations of the Pauli Exclusion Principle (PEP) for electrons, through the search for anomalous X-ray transitions in copper atoms, produced by fresh electrons (brought inside the copper bar by circulating current) which can have the probability to undergo Pauli-forbidden transition to the 1 s level already occupied by two electrons and we describe the VIP2 (VIolation of PEP) experiment under data taking at the Gran Sasso underground laboratories. In this paper the new VIP2 setup installed in the Gran Sasso underground laboratory will be presented. The goal of VIP2 is to test the PEP for electrons with unprecedented accuracy, down to a limit in the probability that PEP is violated at the level of...

The PEP II injection kicker system

International Nuclear Information System (INIS)

Pappas, G.C.; Donaldson, A.R.; Williams, D.

1997-07-01

PEP II or the B Factory consists of two asymmetric storage rings. The injection energy for electrons is 9 GeV, while that for positrons is 3.1 GeV. The bend angle into the high energy ring (HER) is 0.35 m-rad, and the angle into the low energy ring (LER) is 0.575 m-rad. The magnetic length for the HER kicker is 0.85 m, and 0.55 m for the LER kicker. The field produced by the magnet is therefore 123.5 G for the HER, and 132 G for the LER. Each ring has a kicker magnet upstream of the injection line which is used to distort the orbit of the stored beam. An identical magnet downstream of the injection line is used to restore the orbit of the stored beam and inject the incoming beam. The two magnets are driven in parallel by the modulator. The apeture of the magnets is 3.86x3.46 cm (HxV). Therefore the current required to drive the HER is 863 A, while for the LER it is 756 A. The inductance of the magnet is approximately 1.4 uH/m. The current pulse is a critically damped sinusoid with a rise time of less than 300 ns. A kicker system has been designed which can be used for injection of both beams by varying the charge of voltage. The modulator uses a conjugate circuit to match the impedance of the magnet, and coupling to the beam chamber

Trickle-Charge: a New Operational Mode for PEP-II

International Nuclear Information System (INIS)

Kozanecki, W.; Colocho, W.S.; Decker, F.-J.; Ecklund, S.; Fisher, A.S.; Iverson, R.H.; O'Grady, C.; Seeman, J.; Sullivan, M.K.; Turner, J.L.; Weaver, M.; Wienands, U.; SLAC

2005-01-01

In regular top-up-and-coast operation, PEP-II average luminosity is about 70-75% of the peak luminosity due to detector ramp-down and ramp-up times plus the time it takes to top-up both beams. We recently commissioned a new operational mode where the Low Energy Ring is injected continuously without ramping down the detector. The benefits--increased luminosity lifetime and roughly half the number of top-ups per shift--were expected to give an increase in delivered luminosity of about 15% at the same peak luminosity; this was confirmed in test runs. In routine trickle operation, however, it appears that the increase in delivered luminosity is more than twice that due to an increase in availability credited to the more stable operating conditions during trickle operation. Further gains were made when continuous injection was extended to the high energy ring as well. In this paper we will present our operational experience as well as some of the diagnostics we use to monitor and maintain tuning of the machine in order to control injection background and protect the detector

PEP-II Large Power Supplies Rebuild Program at SLAC

CERN Document Server

de Lira, Antonio C; Lipari, James J; da Silva Rafael, Fernando

2005-01-01

At PEP-II, seven large power supplies (LGPS) are used to power quad magnets in the electron-positron collider region. The LGPS ratings range from 72kW to 270kW, and were installed in 1997. They are unipolar off-line switch mode supplies, with a 6 pulse bridge rectifying 480VAC, 3-phase input power to yield 650VDC unregulated. This unregulated 650VDC is then input into one (or two) IGBT H-bridges, which convert the DC into PWM 16 kHz square wave AC. This high frequency AC drives the primary side of a step-down transformer followed by rectifiers and low pass filters. Over the years, these LGPS have presented many problems mainly in their control circuits, making it difficult to troubleshoot and affecting the overall accelerator availability. A redesign/rebuilding program for these power supplies was established under the coordination of the Power Conversion Department at SLAC. During the 2004 accelerator summer shutdown all the control circuits in these supplies were redesigned and replaced. A new PWM control b...

Lattice Effects Due to High Currents in PEP-II

International Nuclear Information System (INIS)

Decker, F.-J.; Smith, H.; Turner, J.L.; SLAC

2005-01-01

The very high beam currents in the PEP-II B-Factory have caused many expected and unexpected effects: Synchrotron light fans move the beam pipe and cause dispersion; higher order modes cause excessive heating, e-clouds around the positron beam blow up its beam size. Here we describe an effect where the measured dispersion of the beam in the Low Energy Ring (LER) is different at high and at low beam currents. The dispersion was iteratively lowered by making anti-symmetric orbit bumps in many sextupole duplets, checking each time with a dispersion measurement where a dispersive kick is generated. This can be done parasitically during collisions. It was a surprise when checking the low current characterization data that there is a change. Subsequent high and low current measurements confirmed the effect. One source was believed to be located far away from any synchrotron radiation in the middle of a straight (PR12), away from sextupoles and skew quadrupoles and created a dispersion wave of about 70 mm at high current while at low current it is negligible

ZAP study of collective effects in PEP: 9 x 9 collider optics

International Nuclear Information System (INIS)

Corbett, W.J.

1990-02-01

In this note, single and multi-bunch collective instabilities are considered for PEP operating a 9 x 9 bunch configuration. The lattice is based on a vertically separated beam 'pretzel' design which allows for collisions at the TPC (IR2) only. Threshold current levels and linear instability growth rates are calculated with the storage ring design code 'ZAP'. Single bunch instabilities should not be a problem for total circulating currents of 100mA (18 bunches, 5.6mA/bunch). Coupled-bunch growth rate calculations are based on a line broadening technique for the higher-order cavity modes. In the longitudinal case, feedback will be required. For the transverse coupled bunch instabilities, growth rates are about 5 times less. 14 refs., 5 figs

Low-energy solar neutrino spectroscopy with Borexino. Towards the detection of the solar pep and CNO neutrino flux

Energy Technology Data Exchange (ETDEWEB)

Maneschg, Werner

2011-05-11

Borexino is a large-volume organic liquid scintillator detector of unprecedented high radiopurity which has been designed for low-energy neutrino spectroscopy in real time. Besides the main objective of the experiment, the measurement of the solar {sup 7}Be neutrino flux, Borexino also aims at detecting solar neutrinos from the pep fusion process and from the CNO cycle. The detectability of these neutrinos is strictly connected to a successful rejection of all relevant background components. The identification and reduction of these background signals is the central subject of this dissertation. In the first part, contaminants induced by cosmic-ray muons and muon showers were analyzed. The dominant background is the cosmogenic radioisotope {sup 11}C. Its rate is {proportional_to}10 times higher than the expected combined pep and CNO neutrino rate in the preferred energy window of observation at [0.8,1.3] MeV. Since {sup 11}C is mostly produced under the release of a free neutron, {sup 11}C can be tagged with a threefold coincidence (TFC) consisting of the muon signal, the neutron capture and the subsequent {sup 11}C decay. By optimizing the TFC method and other rejection techniques, a {sup 11}C rejection efficiency of 80% was achieved. This led to a neutrino-to-background ratio of 1:1.7, whereby 61% of statistics is lost. The second part of the work concerns the study of the external background. Especially long-range 2.6 MeV gamma rays from {sup 208}Tl decays in the outer detector parts can reach the scintillator in the innermost region of the detector. For the determination of the resultant spectral shape, a custom-made {proportional_to}5 MBq {sup 228}Th source was produced and an external calibration was carried out for the first time. The obtained calibration data and the achieved {sup 11}C rejection efficiency will allow for the direct detection of solar pep and possibly also CNO neutrinos with Borexino. (orig.)

Low-energy solar neutrino spectroscopy with Borexino. Towards the detection of the solar pep and CNO neutrino flux

International Nuclear Information System (INIS)

Maneschg, Werner

2011-01-01

Borexino is a large-volume organic liquid scintillator detector of unprecedented high radiopurity which has been designed for low-energy neutrino spectroscopy in real time. Besides the main objective of the experiment, the measurement of the solar 7 Be neutrino flux, Borexino also aims at detecting solar neutrinos from the pep fusion process and from the CNO cycle. The detectability of these neutrinos is strictly connected to a successful rejection of all relevant background components. The identification and reduction of these background signals is the central subject of this dissertation. In the first part, contaminants induced by cosmic-ray muons and muon showers were analyzed. The dominant background is the cosmogenic radioisotope 11 C. Its rate is ∝10 times higher than the expected combined pep and CNO neutrino rate in the preferred energy window of observation at [0.8,1.3] MeV. Since 11 C is mostly produced under the release of a free neutron, 11 C can be tagged with a threefold coincidence (TFC) consisting of the muon signal, the neutron capture and the subsequent 11 C decay. By optimizing the TFC method and other rejection techniques, a 11 C rejection efficiency of 80% was achieved. This led to a neutrino-to-background ratio of 1:1.7, whereby 61% of statistics is lost. The second part of the work concerns the study of the external background. Especially long-range 2.6 MeV gamma rays from 208 Tl decays in the outer detector parts can reach the scintillator in the innermost region of the detector. For the determination of the resultant spectral shape, a custom-made ∝5 MBq 228 Th source was produced and an external calibration was carried out for the first time. The obtained calibration data and the achieved 11 C rejection efficiency will allow for the direct detection of solar pep and possibly also CNO neutrinos with Borexino. (orig.)

A tracking system for laboratory mice to support medical researchers in behavioral analysis.

Science.gov (United States)

Macrì, S; Mainetti, L; Patrono, L; Pieretti, S; Secco, A; Sergi, I

2015-08-01

The behavioral analysis of laboratory mice plays a key role in several medical and scientific research areas, such as biology, toxicology, pharmacology, and so on. Important information on mice behavior and their reaction to a particular stimulus is deduced from a careful analysis of their movements. Moreover, behavioral analysis of genetically modified mice allows obtaining important information about particular genes, phenotypes or drug effects. The techniques commonly adopted to support such analysis have many limitations, which make the related systems particularly ineffective. Currently, the engineering community is working to explore innovative identification and sensing technologies to develop new tracking systems able to guarantee benefits to animals' behavior analysis. This work presents a tracking solution based on passive Radio Frequency Identification Technology (RFID) in Ultra High Frequency (UHF) band. Much emphasis is given to the software component of the system, based on a Web-oriented solution, able to process the raw tracking data coming from a hardware system, and offer 2D and 3D tracking information as well as reports and dashboards about mice behavior. The system has been widely tested using laboratory mice and compared with an automated video-tracking software (i.e., EthoVision). The obtained results have demonstrated the effectiveness and reliability of the proposed solution, which is able to correctly detect the events occurring in the animals' cage, and to offer a complete and user-friendly tool to support researchers in behavioral analysis of laboratory mice.

Measurements of a Fast Vertical Instability in the PEP-II HER

International Nuclear Information System (INIS)

Matter, Regina S.

2000-01-01

The HER beam current was limited to tens of mA by a strong vertical coupled bunch instability during PEP-II commissioning in July '98. In the absence of transverse feedback, vertical oscillations were seen to be unstable at currents as low as 5 mA. The instability has been observed by using the HER longitudinal feedback electronics to digitize and store the vertical beam signal used for transverse feedback. This note presents measurements of modal growth and damping rates at two different vacuum pressures for 291 bunch uniform fills as well as 100 and 150 bunch train measurements. In some cases the unstable modes maintain a constant residual amplitude when feedback is on, and exhibit nonlinear behavior when feedback is switched off. Knowledge of the shape, speed and spectral location of the modal transients should help in diagnosing the cause of instability

Successful field and laboratory tests of advanced phytoremediation systems for decontamination of petroleum and salt impacted soils

Energy Technology Data Exchange (ETDEWEB)

Greenberg, B.; Huang, X.D.; Gerhardt, K.; Gurska, J.; Yu, X.M.; MacNeill, G.; Lu, X.; Nykamp, J.; Glick, B.; Wang, W.; Wang, H.; Wu, S.; Knezevich, N.; Gerwing, P. [Waterloo Univ., ON (Canada)]|[Earthmaster Environmental Strategies Inc., Calgary, AB (Canada)]|[Waterloo Environmental Biotechnology Inc., Waterloo, ON (Canada)

2008-07-01

This presentation discussed the advantages of phytoremediation and provided an overview of a phytoremediation tests results for petroleum and salt remediation. Several examples of remediation methods were discussed, including the dig and dump method; soil incineration; chemical extraction; electrokinetic separation and land farming/natural attenuation. The advantages of phytoremediation include improved natural structure and texture of soil; suitability to most regions and climates because it is driven by solar energy; cost effectiveness and technically feasible; reasonable time frame for restoration; promotion of high rhizosphere activity by plants; and effective use at remote sites. The development and proof of plant growth promoting rhizobacteria (PGPR) enhanced phytoremediation systems (PEPS) was then addressed. A description of the PEPS was provided. This presentation also reviewed the interaction of a PGPR containing ACC deaminase with a plant seed or root; research and development of the PEPS for PHC remediation; the use of petroleum remediation on an Imperial Oil Sarnia land farm; proof of concept of the application of the PEPS for PHC remediation in Hinton, Alberta; and development of the PEPS for salt impacted sites. Field work and the characteristics of soils were also examined. It was concluded that the PEPS has great potential for efficient remediation of organic, salt and metal contaminated sites and that PGPR alleviates stress and promotes growth resulting in low ethylene and high auxin content. tabs., figs.

Report on the laboratory examination of the effect of fungal invasion on the durability of untreated mine support timbers

CSIR Research Space (South Africa)

Hall, PJ

1967-02-01

Full Text Available stream_source_info RR 12_67 The laboratory examination of the effect of fungal invasion on the durability of untreated mine support timber COMRO 1967.pdf.txt stream_content_type text/plain stream_size 8 Content-Encoding ISO-8859...-1 stream_name RR 12_67 The laboratory examination of the effect of fungal invasion on the durability of untreated mine support timber COMRO 1967.pdf.txt Content-Type text/plain; charset=ISO-8859-1 ...

Meson and baryon correlation studies using the PEP-TPC/2γ Facility

International Nuclear Information System (INIS)

Ronan, M.T.

1991-03-01

Results on vector meson, and strange and charmed-baryon production are presented for data taken during the period 1982--1986 using the TPC/2γ detector at PEP. Vector mesons (ρ 0 , K * and φ) with 0, 1 and 2 strange quarks are used to obtain redundant measures of strange-quark suppression and of the vector to pseudoscalar ratio in hadronization. Measurements of the production rates of Λ, Ξ - , Ω and Ξ *0 hyperons and for the Λ c and of rapidity correlations between Λ bar Λ pairs provide sensitive tests of baryon production in fragmentation models. In addition, two- and three-particle correlations between like sign pions provide further evidence for the Bose-Einstein effect in e + e - interactions including the relativistic motion of particle sources. 9 refs., 7 figs

Evaluation of quality indicators in a laboratory supporting tertiary cancer care facilities in India.

Science.gov (United States)

Kumar, Savitha Anil; Jayanna, Prashanth; Prabhudesai, Shilpa; Kumar, Ajai

2014-01-01

To collect and tabulate errors and nonconformities in the preanalytical, analytical, and postanalytical process phases in a diagnostic clinical laboratory that supports a super-specialty cancer center in India, and identify areas of potential improvement in patient services. We collected data from our laboratory during a period of 24 months. Departments in the study included clinical biochemistry, hematology, clinical pathology, microbiology and serology, surgical pathology, and molecular pathology. We had initiated quality assessment based on international standards in our laboratory in 2010, with the aim of obtaining accreditation by national and international governing bodies. We followed the guidelines specified by International Organization for Standardization (ISO) 15189:2007 to identify noncompliant elements of our processes. Among a total of 144,030 specimens that our referral laboratory received during the 2-year period of our study, we uncovered an overall error rate for all 3 process phases of 1.23%; all of our error rates closely approximated the results from our peer institutions. Errors were most common in the preanalytical phase in both years of study; preanalytical- and postanalytical-phase errors constituted more than 90% of all errors. Further improvements are warranted in laboratory services and are contingent on adequate training and interdepartmental communication and cooperation. Copyright© by the American Society for Clinical Pathology (ASCP).

Reduced Clostridium difficile Tests and Laboratory-Identified Events With a Computerized Clinical Decision Support Tool and Financial Incentive.

Science.gov (United States)

Madden, Gregory R; German Mesner, Ian; Cox, Heather L; Mathers, Amy J; Lyman, Jason A; Sifri, Costi D; Enfield, Kyle B

2018-06-01

We hypothesized that a computerized clinical decision support tool for Clostridium difficile testing would reduce unnecessary inpatient tests, resulting in fewer laboratory-identified events. Census-adjusted interrupted time-series analyses demonstrated significant reductions of 41% fewer tests and 31% fewer hospital-onset C. difficile infection laboratory-identified events following this intervention.Infect Control Hosp Epidemiol 2018;39:737-740.

Assessment of readiness for clinical decision support to aid laboratory monitoring of immunosuppressive care at U.S. liver transplant centers.

Science.gov (United States)

Jacobs, J; Weir, C; Evans, R S; Staes, C

2014-01-01

Following liver transplantation, patients require lifelong immunosuppressive care and monitoring. Computerized clinical decision support (CDS) has been shown to improve post-transplant immunosuppressive care processes and outcomes. The readiness of transplant information systems to implement computerized CDS to support post-transplant care is unknown. a) Describe the current clinical information system functionality and manual and automated processes for laboratory monitoring of immunosuppressive care, b) describe the use of guidelines that may be used to produce computable logic and the use of computerized alerts to support guideline adherence, and c) explore barriers to implementation of CDS in U.S. liver transplant centers. We developed a web-based survey using cognitive interviewing techniques. We surveyed 119 U.S. transplant programs that performed at least five liver transplantations per year during 2010-2012. Responses were summarized using descriptive analyses; barriers were identified using qualitative methods. Respondents from 80 programs (67% response rate) completed the survey. While 98% of programs reported having an electronic health record (EHR), all programs used paper-based manual processes to receive or track immunosuppressive laboratory results. Most programs (85%) reported that 30% or more of their patients used external laboratories for routine testing. Few programs (19%) received most external laboratory results as discrete data via electronic interfaces while most (80%) manually entered laboratory results into the EHR; less than half (42%) could integrate internal and external laboratory results. Nearly all programs had guidelines regarding pre-specified target ranges (92%) or testing schedules (97%) for managing immunosuppressive care. Few programs used computerized alerting to notify transplant coordinators of out-of-range (27%) or overdue laboratory results (20%). Use of EHRs is common, yet all liver transplant programs were largely

Mechanical support and transport system used for the neutrino horn system at Brookhaven National Laboratory

International Nuclear Information System (INIS)

Walker, J.C.; Carroll, A.S.; Leonhardt, W.

1987-01-01

The study of neutrinos at the Alternating Gradient Synchrotron (AGS), Brookhaven National Laboratory (BNL), requires hardware for their initiation and control. The basics consist of a target, two horns and three collimators. This paper describes the installation, support and positioning of these components within a settling concrete blockhouse

Comparison of q anti qg and q anti qγ events in e+e- annihilation at PEP

International Nuclear Information System (INIS)

Hofmann, W.

1986-07-01

In comparing the particle flow in the event plane of three-jet (q anti qg) events and of radiative annihilation events (q anti qγ) for similar kinematic configurations, two PEP experiments find a significant decrease in particle density in the angular region opposite to the gluon jet in q anti qg events, relative to the particle density in the region opposite to the photon in q anti qγ events. The effect is predicted both by QCD and by phenomenological string models. 5 refs., 5 figs

Assembly and evaluation of an inventory of guidelines that are available to support clinical hematology laboratory practice.

Science.gov (United States)

Hayward, C P M; Moffat, K A; George, T I; Proytcheva, M

2015-05-01

Practice guidelines provide helpful support for clinical laboratories. Our goal was to assemble an inventory of publically listed guidelines on hematology laboratory topics, to create a resource for laboratories and for assessing gaps in practice-focused guidelines. PubMed and website searches were conducted to assemble an inventory of hematology laboratory-focused guidelines. Exclusions included annual, technical, or collaborative study reports, clinically focused guidelines, position papers, nomenclature, and calibration documents. Sixty-eight guidelines were identified on hematology laboratory practice topics from 12 organizations, some as joint guidelines. The median year of publication was 2010 and 15% were >10 years old. Coagulation topics had the largest numbers of guidelines, whereas some areas of practice had few guidelines. A minority of guidelines showed evidence of periodic updates, as some organizations did not remove or identify outdated guidelines. This inventory of current practice guidelines will encourage awareness and uptake of guideline recommendations by the worldwide hematology laboratory community, with the International Society for Laboratory Hematology facilitating ongoing updates. There is a need to encourage best guideline development practices, to ensure that hematology laboratory community has current, high-quality, and evidence-based practice guidelines that cover the full scope of hematology laboratory practice. © 2015 John Wiley & Sons Ltd.

Rosetta FlexPepDock ab-initio: simultaneous folding, docking and refinement of peptides onto their receptors.

Science.gov (United States)

Raveh, Barak; London, Nir; Zimmerman, Lior; Schueler-Furman, Ora

2011-04-29

Flexible peptides that fold upon binding to another protein molecule mediate a large number of regulatory interactions in the living cell and may provide highly specific recognition modules. We present Rosetta FlexPepDock ab-initio, a protocol for simultaneous docking and de-novo folding of peptides, starting from an approximate specification of the peptide binding site. Using the Rosetta fragments library and a coarse-grained structural representation of the peptide and the receptor, FlexPepDock ab-initio samples efficiently and simultaneously the space of possible peptide backbone conformations and rigid-body orientations over the receptor surface of a given binding site. The subsequent all-atom refinement of the coarse-grained models includes full side-chain modeling of both the receptor and the peptide, resulting in high-resolution models in which key side-chain interactions are recapitulated. The protocol was applied to a benchmark in which peptides were modeled over receptors in either their bound backbone conformations or in their free, unbound form. Near-native peptide conformations were identified in 18/26 of the bound cases and 7/14 of the unbound cases. The protocol performs well on peptides from various classes of secondary structures, including coiled peptides with unusual turns and kinks. The results presented here significantly extend the scope of state-of-the-art methods for high-resolution peptide modeling, which can now be applied to a wide variety of peptide-protein interactions where no prior information about the peptide backbone conformation is available, enabling detailed structure-based studies and manipulation of those interactions. © 2011 Raveh et al.

The Laboratories at Seibersdorf: Multi-disciplinary research and support centre

International Nuclear Information System (INIS)

Danesi, P.R.

1987-01-01

The main research activities performed at the IAEA laboratories at Seibersdorf in the Agriculture Laboratory, Physics-Chemistry-Instrumentation Laboratory and Safeguards Analytical Laboratory, as well as the training activities are briefly described

94-1 Research and development project lead laboratory support. Status report, January 1--March 31, 1997

Energy Technology Data Exchange (ETDEWEB)

Rink, N.A. [comp.

1997-08-01

This status report is published for Los Alamos National Laboratory 94-1 Research and Development Project Support. The Department of Energy Office of Environmental Management funds these projects in order to support the storage or disposal of legacy plutonium and plutonium-bearing materials that resulted from weapons production throughout the DOE complex. This report summarizes status and technical progress for Los Alamos 94-1 projects during the second quarter of fiscal year 1997.

94-1 Research and development project lead laboratory support. Status report, January 1--March 31, 1997

International Nuclear Information System (INIS)

Rink, N.A.

1997-08-01

This status report is published for Los Alamos National Laboratory 94-1 Research and Development Project Support. The Department of Energy Office of Environmental Management funds these projects in order to support the storage or disposal of legacy plutonium and plutonium-bearing materials that resulted from weapons production throughout the DOE complex. This report summarizes status and technical progress for Los Alamos 94-1 projects during the second quarter of fiscal year 1997

The proposed injection system for an asymmetric B Factory in the PEP tunnel

International Nuclear Information System (INIS)

Bloom, E.; Bulos, F.; Loew, G.; Miller, R.; Sukiennicki, B.; Mattison, T.; Barletta, W.

1991-01-01

The proposed asymmetric energy B Factory to be built in the PEP tunnel at SLAC will require a highly effective and profuse source of low emittance electron and positron bunches. The B Factory will consist of two rings of equal size, a 9 GeV electron ring and a 3.1 GeV positron ring, each with 1658 bunches with total circulating currents of 1.5 and 2.1 amperes respectively. As the luminosity lifetime of the collider is expected to be about two hours, the injector should be capable of filling the rings in a small fraction of an hour. It turns out that with some simple modifications, the SLC linac with its damping rings and positron source is ideally suited to fulfill this function effectively. The overall injection system is described

Laboratory work in support of West Valley glass development

International Nuclear Information System (INIS)

Bunnell, L.R.

1988-05-01

Over the past six years, Pacific Northwest Laboratory (PNL) has conducted several studies in support of waste glass composition development and testing of glass compositions suitable for immobilizing the nuclear wastes stored at West Valley, New York. As a result of pilot-scale testing conducted by PNL, the glass composition was changed from that originally recommended in response to changes in the waste stream, and several processing-related problems were discovered. These problems were solved, or sufficiently addressed to determine their likely effect on the glass melting operations to be conducted at West Valley. This report describes the development of the waste glass composition, WV-205, and discusses solutions to processing problems such as foaming and insoluble sludges, as well as other issues such as effects of feed variations on processing of the resulting glass. An evaluation of the WV-205 glass from a repository perspective is included in the appendix to this report

SIAT: Information system of support to the administration of technology; SIAT: Sistema de informacion de apoyo a la administracion de la tecnologia

Energy Technology Data Exchange (ETDEWEB)

Molina Gonzalez, Ricardo; Herrera Lavin, Jesus; Rodriguez Ortiz, Guillermo [Instituto de Investigaciones Electricas, Temixco, Morelos (Mexico)

1999-07-01

A general introduction is made of the administration problem of the technology and a brief description of the process implemented in Petroleos Mexicanos (PEMEX) Exploration and Production (PEP) for the administration of the technology in PEP and the benefits that it provides. [Spanish] Se presenta una introduccion general del problema de administracion de la tecnologia y una breve descripcion del proceso implantado en Petroleos Mexicanos (Pemex) Exploracion y Produccion (PEP) para la administracion de las tecnologias en PEP y los beneficios que proporciona.

Technical Basis of Scaling Relationships for the Pretreatment Engineering Platform

International Nuclear Information System (INIS)

Kuhn, William L.; Arm, Stuart T.; Huckaby, James L.; Kurath, Dean E.; Rassat, Scot D.

2008-01-01

Pacific Northwest National Laboratory has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Waste Treatment Plant (RPP-WTP) project to perform research and development activities. The Pretreatment Engineering Platform (PEP) is being designed and constructed as part of a plan to respond to an issue raised by the WTP External Flowsheet Review Team (EFRT) entitled 'Undemonstrated Leaching Processes' and numbered M12. The PEP replicates the WTP leaching process using prototypic equipment and control strategies. The approach for scaling PEP performance data to predict WTP performance is critical to the successful resolution of the EFRT issue. This report describes the recommended PEP scaling approach, PEP data interpretation and provides recommendations on test conduct and data requirements

Technical Basis of Scaling Relationships for the Pretreatment Engineering Platform

Energy Technology Data Exchange (ETDEWEB)

Kuhn, William L.; Arm, Stuart T.; Huckaby, James L.; Kurath, Dean E.; Rassat, Scot D.

2008-07-15

Pacific Northwest National Laboratory has been tasked by Bechtel National Inc. (BNI) on the River Protection Project-Waste Treatment Plant (RPP-WTP) project to perform research and development activities. The Pretreatment Engineering Platform (PEP) is being designed and constructed as part of a plan to respond to an issue raised by the WTP External Flowsheet Review Team (EFRT) entitled “Undemonstrated Leaching Processes” and numbered M12. The PEP replicates the WTP leaching process using prototypic equipment and control strategies. The approach for scaling PEP performance data to predict WTP performance is critical to the successful resolution of the EFRT issue. This report describes the recommended PEP scaling approach, PEP data interpretation and provides recommendations on test conduct and data requirements.

A search for supersymmetric electrons with the Mark II detector at PEP (Positron Electron Project)

Energy Technology Data Exchange (ETDEWEB)

LeClaire, B.W.

1987-10-01

An experimental search for selectrons, the supersymmetric partner of the electron, has been performed at the PEP storage ring at SLAC using the Mark II detector. The experimental search done was based upon hypothetical reaction in e/sup +/e/sup -/ interactions at PEP center of mass energies of 29 GeV. In this reaction the selectrons, e-tilde, are assumed produced by the interaction of one of initial state electrons with a photon radiated from the other initial state electron. This latter electron is assumed to continue down the beam pipe undetected. The photon and electron then produce a selectron and a photino, ..gamma..-tilde, in the supersymmetric analog of Compton scattering. The photino is assumed to be the lightest supersymmetric particle, and as such, does not interact in the detector, thereby escaping detection very much like a neutrino. The selectron is assumed to immediately decay into an electron and photino. This electron is produced with large p perpendicular with respect to the beam pipe, since it must balance the transverse momentum carried off by the photinos. Thus, the experimental signature of the process is a single electron in the detector with a large unbalanced tranverse momentum. No events of this type were observed in the original search of 123 pb/sup -1/ of data, resulting in a cross section limit of less than 2.4 x 10/sup -2/ pb (at the 95% CL) within the detector acceptance. This cross section upper limit applies to any process which produces anomalous single electron events with missing transverse momentum. When interpreted as a supersymmetry search it results in a lower selectron mass limit of 22.2 GeV/c/sup 2/ for the case of massless photinos. Limits for non-zero mass photinos have been calculated. 87 refs., 67 figs., 17 tabs.

A search for supersymmetric electrons with the Mark II detector at PEP [Positron Electron Project

International Nuclear Information System (INIS)

LeClaire, B.W.

1987-10-01

An experimental search for selectrons, the supersymmetric partner of the electron, has been performed at the PEP storage ring at SLAC using the Mark II detector. The experimental search done was based upon hypothetical reaction in e + e - interactions at PEP center of mass energies of 29 GeV. In this reaction the selectrons, e, are assumed produced by the interaction of one of initial state electrons with a photon radiated from the other initial state electron. This latter electron is assumed to continue down the beam pipe undetected. The photon and electron then produce a selectron and a photino, γ, in the supersymmetric analog of Compton scattering. The photino is assumed to be the lightest supersymmetric particle, and as such, does not interact in the detector, thereby escaping detection very much like a neutrino. The selectron is assumed to immediately decay into an electron and photino. This electron is produced with large p perpendicular with respect to the beam pipe, since it must balance the transverse momentum carried off by the photinos. Thus, the experimental signature of the process is a single electron in the detector with a large unbalanced tranverse momentum. No events of this type were observed in the original search of 123 pb -1 of data, resulting in a cross section limit of less than 2.4 x 10 -2 pb (at the 95% CL) within the detector acceptance. This cross section upper limit applies to any process which produces anomalous single electron events with missing transverse momentum. When interpreted as a supersymmetry search it results in a lower selectron mass limit of 22.2 GeV/c 2 for the case of massless photinos. Limits for non-zero mass photinos have been calculated. 87 refs., 67 figs., 17 tabs

Development of a movable plunger tuner for the high-power RF cavity for the PEP-II B-factory

International Nuclear Information System (INIS)

Schwarz, H.D.; Fant, K.; Judkins, J.G.

1997-05-01

A 10 cm diameter by 5 cm travel plunger tuner was developed for the PEP-II RF copper cavity system. The single cell cavity including the tuner is designed to operate up to 150 kW of dissipated RF power are specially placed 8.5 cm away from the inside wall of the cavity to avoid fundamental and higher order mode resonances. The spring fingers are made of dispersion-strengthened copper to accommodate relatively high heating. The design, alignment, testing and performance of the tuner is described

Integrated safeguards testing laboratories in support of the advanced fuel cycle initiative

International Nuclear Information System (INIS)

Santi, Peter A.; Demuth, Scott F.; Klasky, Kristen L.; Lee, Haeok; Miller, Michael C.; Sprinkle, James K.; Tobin, Stephen J.; Williams, Bradley

2009-01-01

A key enabler for advanced fuel cycle safeguards research and technology development for programs such as the Advanced Fuel Cycle Initiative (AFCI) is access to facilities and nuclear materials. This access is necessary in many cases in order to ensure that advanced safeguards techniques and technologies meet the measurement needs for which they were designed. One such crucial facility is a hot cell based laboratory which would allow developers from universities, national laboratories, and commercial companies to perform iterative research and development of advanced safeguards instrumentation under realistic operating conditions but not be subject to production schedule limitations. The need for such a facility arises from the requirement to accurately measure minor actinide and/or fission product bearing nuclear materials that cannot be adequately shielded in glove boxes. With the contraction of the DOE nuclear complex following the end of the cold war, many suitable facilities at DOE sites are increasingly costly to operate and are being evaluated for closure. A hot cell based laboratory that allowed developers to install and remove instrumentation from the hot cell would allow for both risk mitigation and performance optimization of the instrumentation prior to fielding equipment in facilities where maintenance and repair of the instrumentation is difficult or impossible. These benefits are accomplished by providing developers the opportunity to iterate between testing the performance of the instrumentation by measuring realistic types and amounts of nuclear material, and adjusting and refining the instrumentation based on the results of these measurements. In this paper, we review the requirements for such a facility using the Wing 9 hot cells in the Los Alamos National Laboratory's Chemistry and Metallurgy Research facility as a model for such a facility and describe recent use of these hot cells in support of AFCI.

Integrated safeguards testing laboratories in support of the advanced fuel cycle initiative

Energy Technology Data Exchange (ETDEWEB)

Santi, Peter A [Los Alamos National Laboratory; Demuth, Scott F [Los Alamos National Laboratory; Klasky, Kristen L [Los Alamos National Laboratory; Lee, Haeok [Los Alamos National Laboratory; Miller, Michael C [Los Alamos National Laboratory; Sprinkle, James K [Los Alamos National Laboratory; Tobin, Stephen J [Los Alamos National Laboratory; Williams, Bradley [DOE, NE

2009-01-01

A key enabler for advanced fuel cycle safeguards research and technology development for programs such as the Advanced Fuel Cycle Initiative (AFCI) is access to facilities and nuclear materials. This access is necessary in many cases in order to ensure that advanced safeguards techniques and technologies meet the measurement needs for which they were designed. One such crucial facility is a hot cell based laboratory which would allow developers from universities, national laboratories, and commercial companies to perform iterative research and development of advanced safeguards instrumentation under realistic operating conditions but not be subject to production schedule limitations. The need for such a facility arises from the requirement to accurately measure minor actinide and/or fission product bearing nuclear materials that cannot be adequately shielded in glove boxes. With the contraction of the DOE nuclear complex following the end of the cold war, many suitable facilities at DOE sites are increasingly costly to operate and are being evaluated for closure. A hot cell based laboratory that allowed developers to install and remove instrumentation from the hot cell would allow for both risk mitigation and performance optimization of the instrumentation prior to fielding equipment in facilities where maintenance and repair of the instrumentation is difficult or impossible. These benefits are accomplished by providing developers the opportunity to iterate between testing the performance of the instrumentation by measuring realistic types and amounts of nuclear material, and adjusting and refining the instrumentation based on the results of these measurements. In this paper, we review the requirements for such a facility using the Wing 9 hot cells in the Los Alamos National Laboratory's Chemistry and Metallurgy Research facility as a model for such a facility and describe recent use of these hot cells in support of AFCI.

Laboratory testing of a long expansion rock bolt support for energy-absorbing applications

Directory of Open Access Journals (Sweden)

Skrzypkowski Krzysztof

2018-01-01

Full Text Available The main purpose of rock support and reinforcement in underground mining is to maintain excavations safe and open for their intended lifespan. The basic type of rock mass reinforcement method both in ore and hard coal mining is rock bolt support. Very often, existing bolt support systems are not always capable of providing a reliable controlled performance. Therefore, in recent years energy-absorbing bolts which are exposed to dynamic loading, for example from rock burst caused by high rock stresses, earthquakes, or blasting have appeared. In this article particular attention was paid to short and long expansion bolts. Quasi-static tests of expansion bolts were carried out at the laboratory test facility in simulated mining conditions, especially for the KGHM Polska Miedź S.A. mines. In the underground mines of the Legnica-Głogów Copper District (LGOM the main way to protect the room excavation is rock bolt support with a length from 1.2 m to 2.6 m. Rock bolt support longer than 2.6 m is considered as additional support of excavations and is increasingly being used to reinforce the roofs. The comparisons of energy-absorbing short and long expansion bolts with a length of 1.8m, 3.6m and 5.2m were presented. In addition, for elastic and plastic range of each bolts were determined.

Laboratory testing of a long expansion rock bolt support for energy-absorbing applications

Science.gov (United States)

Skrzypkowski, Krzysztof

2018-01-01

The main purpose of rock support and reinforcement in underground mining is to maintain excavations safe and open for their intended lifespan. The basic type of rock mass reinforcement method both in ore and hard coal mining is rock bolt support. Very often, existing bolt support systems are not always capable of providing a reliable controlled performance. Therefore, in recent years energy-absorbing bolts which are exposed to dynamic loading, for example from rock burst caused by high rock stresses, earthquakes, or blasting have appeared. In this article particular attention was paid to short and long expansion bolts. Quasi-static tests of expansion bolts were carried out at the laboratory test facility in simulated mining conditions, especially for the KGHM Polska Miedź S.A. mines. In the underground mines of the Legnica-Głogów Copper District (LGOM) the main way to protect the room excavation is rock bolt support with a length from 1.2 m to 2.6 m. Rock bolt support longer than 2.6 m is considered as additional support of excavations and is increasingly being used to reinforce the roofs. The comparisons of energy-absorbing short and long expansion bolts with a length of 1.8m, 3.6m and 5.2m were presented. In addition, for elastic and plastic range of each bolts were determined.

National laboratories

International Nuclear Information System (INIS)

Moscati, G.

1983-01-01

The foundation of a 'National Laboratory' which would support a Research center in synchrotron radiation applications is proposed. The essential features of such a laboratory differing of others centers in Brazil are presented. (L.C.) [pt

A high efficiency, high quality and low cost internal regulated bioanalytical laboratory to support drug development needs.

Science.gov (United States)

Song, Yan; Dhodda, Raj; Zhang, Jun; Sydor, Jens

2014-05-01

In the recent past, we have seen an increase in the outsourcing of bioanalysis in pharmaceutical companies in support of their drug development pipeline. This trend is largely driven by the effort to reduce internal cost, especially in support of late-stage pipeline assets where established bioanalytical assays are used to analyze a large volume of samples. This article will highlight our perspective of how bioanalytical laboratories within pharmaceutical companies can be developed into the best partner in the advancement of drug development pipelines with high-quality support at competitive cost.

National Laboratory Planning: Developing Sustainable Biocontainment Laboratories in Limited Resource Areas.

Science.gov (United States)

Yeh, Kenneth B; Adams, Martin; Stamper, Paul D; Dasgupta, Debanjana; Hewson, Roger; Buck, Charles D; Richards, Allen L; Hay, John

2016-01-01

Strategic laboratory planning in limited resource areas is essential for addressing global health security issues. Establishing a national reference laboratory, especially one with BSL-3 or -4 biocontainment facilities, requires a heavy investment of resources, a multisectoral approach, and commitments from multiple stakeholders. We make the case for donor organizations and recipient partners to develop a comprehensive laboratory operations roadmap that addresses factors such as mission and roles, engaging national and political support, securing financial support, defining stakeholder involvement, fostering partnerships, and building trust. Successful development occurred with projects in African countries and in Azerbaijan, where strong leadership and a clear management framework have been key to success. A clearly identified and agreed management framework facilitate identifying the responsibility for developing laboratory capabilities and support services, including biosafety and biosecurity, quality assurance, equipment maintenance, supply chain establishment, staff certification and training, retention of human resources, and sustainable operating revenue. These capabilities and support services pose rate-limiting yet necessary challenges. Laboratory capabilities depend on mission and role, as determined by all stakeholders, and demonstrate the need for relevant metrics to monitor the success of the laboratory, including support for internal and external audits. Our analysis concludes that alternative frameworks for success exist for developing and implementing capabilities at regional and national levels in limited resource areas. Thus, achieving a balance for standardizing practices between local procedures and accepted international standards is a prerequisite for integrating new facilities into a country's existing public health infrastructure and into the overall international scientific community.

Optics/Optical Diagnostics Laboratory

Data.gov (United States)

Federal Laboratory Consortium — The Optics/Optical Diagnostics Laboratory supports graduate instruction in optics, optical and laser diagnostics and electro-optics. The optics laboratory provides...

Vehicle Development Laboratory

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Federal Laboratory Consortium — FUNCTION: Supports the development of prototype deployment platform vehicles for offboard countermeasure systems.DESCRIPTION: The Vehicle Development Laboratory is...

Several hPepT1-transported drugs are substrates of the Escherichia coli proton-coupled oligopeptide transporter YdgR.

Science.gov (United States)

Prabhala, Bala K; Aduri, Nanda G; Iqbal, Mazhar; Rahman, Moazur; Gajhede, Michael; Hansen, Paul R; Mirza, Osman

2017-06-01

Proton-dependent oligopeptide transporters (POTs) are secondary active transporters found in all kingdoms of life. POTs utilize the proton electrochemical gradient for the uptake of nutrient dipeptides and tripeptides. The human POT hPepT1 is known to transport a number of drugs. As part of ongoing studies on substrate specificities of POTs from Escherichia coli, our aim in this study was to investigate whether bacterial POTs could also transport these drugs. For this, we selected the common orally administered drugs sulpiride, bestatin, valacyclovir, ampicillin and oseltamivir, that are all transported by hPepT1. The transport of these drugs was evaluated using the prototypical POT YdgR from E. coli. The transport studies were pursued through combining cell-based assays with liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis. These investigations revealed that YdgR from E. coli is able to transport five (sulpiride, bestatin, valacyclovir, ampicillin and oseltamivir) drugs. Furthermore, cells not overexpressing YdgR were also able to transport these drugs in a POT-like manner. Orthologues of YdgR are found in several species in the gut microbiome; hence, our findings could have implications for further understanding about the interaction between gut microbes and orally administered drugs. Copyright © 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

The Prevention Program for Externalizing Problem Behavior (PEP) Improves Child Behavior by Reducing Negative Parenting: Analysis of Mediating Processes in a Randomized Controlled Trial

Science.gov (United States)

Hanisch, Charlotte; Hautmann, Christopher; Plück, Julia; Eichelberger, Ilka; Döpfner, Manfred

2014-01-01

Background: Our indicated Prevention program for preschool children with Externalizing Problem behavior (PEP) demonstrated improved parenting and child problem behavior in a randomized controlled efficacy trial and in a study with an effectiveness design. The aim of the present analysis of data from the randomized controlled trial was to identify…

Herencia de la resistencia al virus del mosaico deformante del pimentón PepDMV en Capsicum Inheritance of resistance to the deforming pepper mosaic virus, PepDMV, in Capsicum

Directory of Open Access Journals (Sweden)

Mario Augusto García Dávila

2009-10-01

Full Text Available En busca de determinar el modo de herencia de la resistencia en tres materiales resistentes, se hicieron cruzamientos hacia tres líneas susceptibles. Se siguió el método del retrocruzamiento propuesto por Warner (1952 fundamentado en el modelo matemático de Fisher Immer y Tedin (1932 descrito con detalle por Mater (1949 y Jenkins (1982, 1984 en el cual se descompone la varianza genética en tres componentes: aditiva, dominancia y epistática. El modelo del retrocruzamiento incluye los dos parentales, el hibrido de la primera generación y la autofecunfación de la F1 para formar la población F2 y las retrocruzas hacia ambos padres. Las poblaciones fueron evaluadas a resistencia al virus del mosaico del pimentón PepDMV en condiciones de invernadero. Los resultados mostraron que el modelo aditivodominancia explicó la resistencia en los híbridos formados entre materiales resistentes y susceptibles. Los genes con acción heredable transmiten a la descendencia el efecto de resistencia. La ganancia de la resistencia viral se da por la presencia de parentales resistentes.With the aim to determine the mode of inheritance in three resistant materials, crosses were performed towards three susceptible lines. We followed the backcrossing method used by Warner (1952, based on the mathematical model of Fisher Immer and tedin (1932, described in detail by Mater (1949 and Jenkins (1982, 1984. In this method the genetic variance is partitioned into three components: additive, dominant and epistatic. The backcross method includes the two parents, the F1 hybrid, and the self-pollination of the F1 to form the F2 population, and the backcrosses to both parents. The populations were evaluated for resistance to the pepper mosaic virusPepDMV in greenhouse conditions. The results showed that the model additive-dominant explained the resistance in the hybrids formed between resistant and susceptible material. The genes with heritable action transmit to their

Intelligent Optics Laboratory

Data.gov (United States)

Federal Laboratory Consortium — The Intelligent Optics Laboratory supports sophisticated investigations on adaptive and nonlinear optics; advancedimaging and image processing; ground-to-ground and...

Synthesis and labelling of Df-DUPA-Pep with gallium-68 and zirconium-89 as new PSMA ligands

International Nuclear Information System (INIS)

Benjamin Baur; Ehab Al-Momani; Noeen Malik; Hans-Juergen Machulla; Reske, S.N.; Christoph Solbach; Elena Andreolli; University of Milano-Bicocca, Milan

2014-01-01

Prostate-specific membrane antigen (PSMA) is a cell surface protein that is overexpressed in prostate cancer. Due to the specificity of expression of PSMA, numerous urea-based ligands have been synthesized until now. In the current study, we describe the coupling of the chelator desferrioxamine to DUPA-Pep and the subsequent labelling with Ga-68 and Zr-89 as new PSMA ligands. The labelling step is performed at room temperature and pH 7.5. In both radiosyntheses, the RCYs were higher than 95 % within 10 min, making dispensable any further purification and providing the radiotracers directly applicable for further utilization. (author)

Central Laboratories Services

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Federal Laboratory Consortium — The TVA Central Laboratories Services is a comprehensive technical support center, offering you a complete range of scientific, engineering, and technical services....

Embedded Processor Laboratory

Data.gov (United States)

Federal Laboratory Consortium — The Embedded Processor Laboratory provides the means to design, develop, fabricate, and test embedded computers for missile guidance electronics systems in support...

Study of an instability of the PEP-II positron beam (Ohmi effect and multipactoring)

International Nuclear Information System (INIS)

Heifets, S.A.

1995-11-01

The paper is organized in the following way. First, Ohmi effect induced by direct flow of primary photoelectrons is studied for the PEP-II parameters. The production rate and kinematics take into account the antechamber of the LER. We discuss the effect of the secondary emission of electrons in the AL chamber, where the yield is larger than one. Resonance multipactoring is considered, and then the average density of the secondary electrons is estimated taking into account the space-charge effect and the interaction with the beam. We show that in the extreme case there is a self-consistent regime similar to the regime of the space-charge dominated cathode. Finally, the rate of ion production by accumulated electrons and the possibility of the ion induced pressure instability is discussed

A Laboratory Test Expert System for Clinical Diagnosis Support in Primary Health Care

Directory of Open Access Journals (Sweden)

Rodrigo Fernandez-Millan

2015-08-01

Full Text Available Clinical Decision Support Systems have the potential to reduce lack of communication and errors in diagnostic steps in primary health care. Literature reports have showed great advances in clinical decision support systems in the recent years, which have proven its usefulness in improving the quality of care. However, most of these systems are focused on specific areas of diseases. In this way, we propose a rule-based expert system, which supports clinicians in primary health care, providing a list of possible diseases regarding patient’s laboratory tests results in order to assist previous diagnosis. Our system also allows storing and retrieving patient’s data and the history of patient’s analyses, establishing a basis for coordination between the various health care levels. A validation step and speed performance tests were made to check the quality of the system. We conclude that our system could improve clinician accuracy and speed, resulting in more efficiency and better quality of service. Finally, we propose some recommendations for further research.

Observation of Beam Size Flip-Flop in PEP-II

International Nuclear Information System (INIS)

Holtzapple, Robert luther

2002-01-01

The asymmetric B-factory, PEP-II, has delivered a peak luminosity of 4.6 x 10 33 cm -2 s -1 with less than half the design number of bunches, requiring a luminosity per bunch crossing more than three times larger than the design. As a result, strong beam-beam effects are present. The strong beam-beam forces between colliding electron and positron bunches can result in a ''flip-flop'' of the transverse beam size of some bunches. Focusing on one positron-electron colliding bunch pair, a flip-flop occurs when the transverse size of the positron bunch shrinks and the electron bunch grows. The flip-flop accounts for a reduction in luminosity, a lower positron lifetime, and increased background in the BABAR detector. The flip-flop phenomenon occurs not for all of the colliding bunches, but for the bunches at the front of a mini-train. Once a colliding pair has flipped to its reduced luminosity state it can be changed back to its normal state by raising the horizontal tune in the low-energy ring (LER, positrons) by 0.01. Afterwards the LER x-tune can be reduced nearly back to its original point, resulting in higher luminosity. These observations were verified and quantified with a new time-gated camera with a resolution of 2 ns, making it possible to observe single bunches

Design of the PEP-II Interaction Region Septum Quadrupole

Science.gov (United States)

Osborn, J.; Tanabe, J.; Yee, D.; Younger, F.

1997-05-01

The PEP-II QF2 magnet is one of the final focus quadrupoles for the Low-Energy Ring (LER) and utilizes a septum aperture to accommodate the adjacent High-Energy Ring (HER) beamline. The LER lattice design specification calls for an extremely high field quality for this magnet. A conventional water-cooled copper coil and laminated steel core design was selected to allow adjustment in the excitation. The close proximity between the LER and HER beamlines and the required integrated quadrupole strength result in a moderately high current density septum design. The QF2 magnets are imbedded in a confined region at each end of the BaBar detector, thus requiring a small magnet core cross section. Pole face windings are included in the QF2 design to buck the skew octupole term induced by the solenoidal fringe field that leaks out of the detector. Back-leg windings are included to buck a small dipole component induced by the lack of perfect quadrupole symmetry in this septum design. 2D pole contour optimization and 3D end chamfers are used to minimize harmonic errors; a separate permanent-magnet Harmonic Corrector Ring compensates for remaining field errors. The design methods and approach, 2D and 3D analyses, and the resulting expected magnet performance are described in this paper.

Sandia National Laboratories support of the Iraq Nuclear Facility Dismantlement and Disposal Program.

Energy Technology Data Exchange (ETDEWEB)

Cochran, John Russell; Danneels, Jeffrey John

2009-03-01

Because of past military operations, lack of upkeep and looting there are now enormous radioactive waste problems in Iraq. These waste problems include destroyed nuclear facilities, uncharacterized radioactive wastes, liquid radioactive waste in underground tanks, wastes related to the production of yellow cake, sealed radioactive sources, activated metals and contaminated metals that must be constantly guarded. Iraq currently lacks the trained personnel, regulatory and physical infrastructure to safely and securely manage these facilities and wastes. In 2005 the International Atomic Energy Agency (IAEA) agreed to organize an international cooperative program to assist Iraq with these issues. Soon after, the Iraq Nuclear Facility Dismantlement and Disposal Program (the NDs Program) was initiated by the U.S. Department of State (DOS) to support the IAEA and assist the Government of Iraq (GOI) in eliminating the threats from poorly controlled radioactive materials. The Iraq NDs Program is providing support for the IAEA plus training, consultation and limited equipment to the GOI. The GOI owns the problems and will be responsible for implementation of the Iraq NDs Program. Sandia National Laboratories (Sandia) is a part of the DOS's team implementing the Iraq NDs Program. This report documents Sandia's support of the Iraq NDs Program, which has developed into three principal work streams: (1) training and technical consultation; (2) introducing Iraqis to modern decommissioning and waste management practices; and (3) supporting the IAEA, as they assist the GOI. Examples of each of these work streams include: (1) presentation of a three-day training workshop on 'Practical Concepts for Safe Disposal of Low-Level Radioactive Waste in Arid Settings;' (2) leading GOI representatives on a tour of two operating low level radioactive waste disposal facilities in the U.S.; and (3) supporting the IAEA's Technical Meeting with the GOI from April 21

Geometric Design Laboratory

Data.gov (United States)

Federal Laboratory Consortium — Purpose: The mission of the Geometric Design Laboratory (GDL) is to support the Office of Safety Research and Development in research related to the geometric design...

Argonne National Laboratory: Laboratory Directed Research and Development FY 1993 program activities. Annual report

Energy Technology Data Exchange (ETDEWEB)

None

1993-12-23

The purposes of Argonne`s Laboratory Directed Research and Development (LDRD) Program are to encourage the development of novel concepts, enhance the Laboratory`s R&D capabilities, and further the development of its strategic initiatives. Projects are selected from proposals for creative and innovative R&D studies which are not yet eligible for timely support through normal programmatic channels. Among the aims of the projects supported by the Program are establishment of engineering ``proof-of-principle`` assessment of design feasibility for prospective facilities; development of an instrumental prototype, method, or system; or discovery in fundamental science. Several of these projects are closely associated with major strategic thrusts of the Laboratory as described in Argonne`s Five Year Institutional Plan, although the scientific implications of the achieved results extend well beyond Laboratory plans and objectives. The projects supported by the Program are distributed across the major programmatic areas at Argonne as indicated in the Laboratory LDRD Plan for FY 1993.

Changing the PEP-II Center-of-Mass Energy Down to 10 GeV and up to 11 GeV

International Nuclear Information System (INIS)

Sullivan, M.

2009-01-01

PEP-II, the SLAC, LBNL, LLNL B-Factory was designed and optimized to run at the Upsilon 4S resonance (10.580 GeV with an 8.973 GeV e- beam and a 3.119 GeV e+ beam). The interaction region (IR) used permanent magnet dipoles to bring the beams into a head-on collision. The first focusing element for both beams was also a permanent magnet. The IR geometry, masking, beam orbits and beam pipe apertures were designed for 4S running. Even though PEP-II was optimized for the 4S, we successfully changed the center-of-mass energy (E cm ) down to the Upsilon 2S resonance and completed an E cm scan from the 4S resonance up to 11.2 GeV. The luminosity throughout most of these changes remained near 1 x 10 34 cm -2 s -1 . The E cm was changed by moving the energy of the high-energy beam (HEB). The beam energy differed by more than 20% which produced significantly different running conditions for the RF system. The energy loss per turn changed 2.5 times over this range. We describe how the beam energy was changed and discuss some of the consequences for the beam orbit in the interaction region. We also describe some of the RF issues that arose and how we solved them as the high-current HEB energy changed

Time-dependent density functional theory/discrete reaction field spectra of open shell systems : The visual spectrum of [Fe-III(PyPepS)(2)](-) in aqueous solution

NARCIS (Netherlands)

van Duijnen, Piet Th.; Greene, Shannon N.; Richards, Nigel G. J.

2007-01-01

We report the calculated visible spectrum of [Fe-III(PyPepS)(2)](-) in aqueous solution. From all-classical molecular dynamics simulations on the solute and 200 water molecules with a polarizable force field, 25 solute/solvent configurations were chosen at random from a 50 ps production run and

Increasing the Presence of Underrepresented Minorities in the Geosciences: The Woods Hole Partnership Education Program Model and Outcomes

Science.gov (United States)

George, A.; Gutierrez, B.; Jearld, A.; Liles, G.; Scott, O.; Harden, B.

2017-12-01

Launched in 2009, the Partnership Education Program (PEP) is supported by six scientific institutions in Woods Hole, Massachusetts through the Woods Hole Diversity Initiative. PEP, which was shaped by experience with other diversity programs as well as input from scientists in Woods Hole, is designed to promote a diverse scientific community by recruiting talent from minority groups that are under-represented in marine and environmental sciences. Focused on college juniors and seniors with course work in marine and/or environmental sciences, PEP is comprised of a four-week course, "Ocean and Environmental Sciences: Global Climate Change," and a six to eight week individual research project under the guidance of a research mentor. Investigators from the six science institutions serve as course faculty and research mentors. Course credit is through PEP's academic partner, the University of Maryland Eastern Shore. PEP students also participate in seminars, workshops, field trips, at-sea experiences, career development activities, and attend lectures at participating science institutions throughout the summer. Students present their research results at the end of the summer with a 15-minute public presentation. A number of PEP participants then presented their work at professional and scientific meetings, such as AGU, using the program as a gateway to graduate education and career opportunities in the marine and environmental sciences. From 2009 through 2017, 138 students from 86 colleges and universities, including many that previously had sent few or no students or faculty to Woods Hole, have participated in the program. Participating organizations are: Northeast Fisheries Science Center (NOAA Fisheries), Marine Biological Laboratory (MBL), Sea Education Association (SEA), U.S. Geological Survey (USGS), Woods Hole Oceanographic Institution (WHOI), Woods Hole Research Center (WHRC), and University of Maryland Eastern Shore (UMES) - academic partner.

MicroArray Facility: a laboratory information management system with extended support for Nylon based technologies

Directory of Open Access Journals (Sweden)

Beaudoing Emmanuel

2006-09-01

Full Text Available Abstract Background High throughput gene expression profiling (GEP is becoming a routine technique in life science laboratories. With experimental designs that repeatedly span thousands of genes and hundreds of samples, relying on a dedicated database infrastructure is no longer an option. GEP technology is a fast moving target, with new approaches constantly broadening the field diversity. This technology heterogeneity, compounded by the informatics complexity of GEP databases, means that software developments have so far focused on mainstream techniques, leaving less typical yet established techniques such as Nylon microarrays at best partially supported. Results MAF (MicroArray Facility is the laboratory database system we have developed for managing the design, production and hybridization of spotted microarrays. Although it can support the widely used glass microarrays and oligo-chips, MAF was designed with the specific idiosyncrasies of Nylon based microarrays in mind. Notably single channel radioactive probes, microarray stripping and reuse, vector control hybridizations and spike-in controls are all natively supported by the software suite. MicroArray Facility is MIAME supportive and dynamically provides feedback on missing annotations to help users estimate effective MIAME compliance. Genomic data such as clone identifiers and gene symbols are also directly annotated by MAF software using standard public resources. The MAGE-ML data format is implemented for full data export. Journalized database operations (audit tracking, data anonymization, material traceability and user/project level confidentiality policies are also managed by MAF. Conclusion MicroArray Facility is a complete data management system for microarray producers and end-users. Particular care has been devoted to adequately model Nylon based microarrays. The MAF system, developed and implemented in both private and academic environments, has proved a robust solution for

Wiki Laboratory Notebooks: Supporting Student Learning in Collaborative Inquiry-Based Laboratory Experiments

Science.gov (United States)

Lawrie, Gwendolyn Angela; Grøndahl, Lisbeth; Boman, Simon; Andrews, Trish

2016-01-01

Recent examples of high-impact teaching practices in the undergraduate chemistry laboratory that include course-based undergraduate research experiences and inquiry-based experiments require new approaches to assessing individual student learning outcomes. Instructors require tools and strategies that can provide them with insight into individual…

Coaxial wire impedance measurements of BPM buttons for the PEP-II B- factory

International Nuclear Information System (INIS)

Corlett, J.N.

1995-09-01

The coaxial wire impedance measurement uses a conducting rod placed along the beam axis in the vacuum chamber, forming the center conductor in a coaxial line system. Tapers at either end of this section allow for smooth impedance transformation from the 50Ω lines used in common microwave measurement equipment, to the characteristic impedance of the vacuum chamber and center conductor, typically around 200Ω. RF and microwave absorptive material placed in the ends of the vacuum chamber and in the impedance matching tapers minimizes reflections which cause trapped modes within the apparatus, allowing measurements to be made above the traveling-wave cut-off frequency of the vacuum vessel (typically 2.5 - 3.0 GHz for PEP-II). A smooth vessel of the same cross-section as that containing the device under test is used in a reference measurement Resonances within the apparatus are difficult to avoid completely and require careful placing of absorptive material, manufacture of test and reference chambers, and assembly of apparatus

Energetic materials research and development activities at Sandia National Laboratories supported under DP-10 programs

Energy Technology Data Exchange (ETDEWEB)

Ratzel, A.C. III

1998-09-01

This report provides summary descriptions of Energetic Materials (EM) Research and Development activities performed at Sandia National Laboratories and funded through the Department of Energy DP-10 Program Office in FY97 and FY98. The work falls under three major focus areas: EM Chemistry, EM Characterization, and EM Phenomenological Model Development. The research supports the Sandia component mission and also Sandia's overall role as safety steward for the DOE Nuclear Weapons Complex.

Using Elearning techniques to support problem based learning within a clinical simulation laboratory.

Science.gov (United States)

Docherty, Charles; Hoy, Derek; Topp, Helena; Trinder, Kathryn

2004-01-01

This paper details the results of the first phase of a project that used eLearning to support students' learning within a simulated environment. The locus was a purpose built Clinical Simulation Laboratory (CSL) where the School's newly adopted philosophy of Problem Based Learning (PBL) was challenged through lecturers reverting to traditional teaching methods. The solution, a student-centred, problem-based approach to the acquisition of clinical skills was developed using learning objects embedded within web pages that substituted for lecturers providing instruction and demonstration. This allowed lecturers to retain their facilitator role, and encouraged students to explore, analyse and make decisions within the safety of a clinical simulation. Learning was enhanced through network communications and reflection on video performances of self and others. Evaluations were positive, students demonstrating increased satisfaction with PBL, improved performance in exams, and increased self-efficacy in the performance of nursing activities. These results indicate that an elearning approach can support PBL in delivering a student centred learning experience.

Project Execution Plan Project 98L-EWW-460 Plutonium Stabilization and Handling DOE 98-D-453

International Nuclear Information System (INIS)

MCGRATH, G.M.

2000-01-01

This Project Execution Plan (PEP) describes the management methods and responsibilities of the project participants. Project W-460 is sufficiently large to warrant a stand alone PEP. This project specific PEP describes the relationships and responsibilities of the project team and identifies the technical, schedule, and cost baselines that have been established for the project. The Department of Energy (DOE), Hanford Works (Hanford), at Richland, Wa. currently does not have a system capable of stabilizing or packaging large quantities of plutonium-bearing solids to meet DOE technical standard DOE-STD-3013-99. This project will allow Hanford to meet this standard by installing stabilization and packaging equipment (SPE). The SPE is capable of stabilizing and packaging the current inventory of greater than 30 percent plutonium-bearing materials currently stored in the Plutonium Finishing Plant's (PFP) vaults into 3013 storage containers. The scope of this project is to procure and install the SPE via a Hanford contract and coordination with the Savannah River Site. In addition, the project will modify PFP vaults and upgrade the PFP Laboratory measurement systems. The Facility infrastructure will be modified to support the new SPE system and the new standardized storage container configuration

Project Execution Plan Project 98L-EWW-460 Plutonium Stabilization and Handling DOE 98-D-453

Energy Technology Data Exchange (ETDEWEB)

HOLSTEIN, W.A.

1999-08-01

This Project Execution Plan (PEP) describes the management methods and responsibilities of the project participants. Project W-460 is sufficiently large to warrant a stand alone PEP. This project specific PEP describes the relationships and responsibilities of the project team and identifies the technical, schedule, and cost baselines that have been established for the project. The Department of Energy (DOE), Hanford Works (Hanford), at Richland Wa. currently does not have a system capable of stabilizing or packaging large quantities of plutonium-bearing solids to meet DOE technical standard DOE-STD-3013-96. This project will allow Hanford to meet this standard by installing stabilization and packaging equipment (SPE). The SPE is capable of stabilizing and packaging the current inventory of greater than 50 percent plutonium-bearing materials currently stored in the Plutonium Finishing Plant's (PFP) vaults into 3013 storage containers. The scope of this project is to procure and install the SPE via a Hanford contract and coordination with the Savannah River Site. In addition, the project will modify PFP vaults and upgrade the PFP Laboratory measurement systems. The Facility infrastructure will be modified to support the new SPE system and the new standardized storage container configuration.

Project Execution Plan Project 98L-EWW-460 Plutonium Stabilization and Handling DOE 98-D-453

Energy Technology Data Exchange (ETDEWEB)

MCGRATH, G.M.

2000-06-21

This Project Execution Plan (PEP) describes the management methods and responsibilities of the project participants. Project W-460 is sufficiently large to warrant a stand alone PEP. This project specific PEP describes the relationships and responsibilities of the project team and identifies the technical, schedule, and cost baselines that have been established for the project. The Department of Energy (DOE), Hanford Works (Hanford), at Richland, Wa. currently does not have a system capable of stabilizing or packaging large quantities of plutonium-bearing solids to meet DOE technical standard DOE-STD-3013-99. This project will allow Hanford to meet this standard by installing stabilization and packaging equipment (SPE). The SPE is capable of stabilizing and packaging the current inventory of greater than 30 percent plutonium-bearing materials currently stored in the Plutonium Finishing Plant's (PFP) vaults into 3013 storage containers. The scope of this project is to procure and install the SPE via a Hanford contract and coordination with the Savannah River Site. In addition, the project will modify PFP vaults and upgrade the PFP Laboratory measurement systems. The Facility infrastructure will be modified to support the new SPE system and the new standardized storage container configuration.

Design and analysis of a wiggler magnet system for the PEP-II B-Factory LER

International Nuclear Information System (INIS)

Heim, J.; Kendall, M.; Bertolini, L.; Fackler, O.; O'Connor, T.; Swan, T.; Zholents, A.

1996-01-01

The Low Energy Ring (LER) of the PEP-II B-Factory will use a wiggler magnet system for emittance control and additional damping. The wiggler magnet system is a set of 11 individual iron core, water cooled, dipole magnets designed to operate at 1.6 T and generate 400 kW of synchrotron radiation. Space has been provided to add a second wiggler with an additional 400 kW of synchrotron radiation if more damping is needed in the future. A copper vacuum chamber is used with continuous antechambers connected to both sides of the beam chamber via slots. Synchrotron radiation dump surfaces and distributed vacuum pumping are located in both antechambers. The authors describe the design and analysis of the wiggler magnets and the salient features of the vacuum chamber and dumps

Virtual Training Devices Laboratory

Data.gov (United States)

Federal Laboratory Consortium — The Virtual Training Devices (VTD) Laboratory at the Life Cycle Software Engineering Center, Picatinny Arsenal, provides a software testing and support environment...

94-1 Research and Development Project Lead laboratory support. Status report, October 1--December 31, 1995

Energy Technology Data Exchange (ETDEWEB)

Dinehart, M. [comp.

1996-05-01

This is a quarterly progress report of the 94-1 Research and Development Lead Laboratory Support Technical Program Plan for the first quarter of FY 1996. The report provides details concerning descriptions, DOE-complex-wide material stabilization technology needs, scientific background and approach, progress, benefits to the DOE complex, and collaborations for selected subprojects. An executive summary and report on end-of-quarter spending is included.

94-1 Research and Development Project Lead laboratory support. Status report, October 1--December 31, 1995

International Nuclear Information System (INIS)

Dinehart, M.

1996-05-01

This is a quarterly progress report of the 94-1 Research and Development Lead Laboratory Support Technical Program Plan for the first quarter of FY 1996. The report provides details concerning descriptions, DOE-complex-wide material stabilization technology needs, scientific background and approach, progress, benefits to the DOE complex, and collaborations for selected subprojects. An executive summary and report on end-of-quarter spending is included

Robust Control of PEP Formation Rate in the Carbon Fixation Pathway of C4 Plants by a Bi-functional Enzyme

Directory of Open Access Journals (Sweden)

Hart Yuval

2011-10-01

Full Text Available Abstract Background C4 plants such as corn and sugarcane assimilate atmospheric CO2 into biomass by means of the C4 carbon fixation pathway. We asked how PEP formation rate, a key step in the carbon fixation pathway, might work at a precise rate, regulated by light, despite fluctuations in substrate and enzyme levels constituting and regulating this process. Results We present a putative mechanism for robustness in C4 carbon fixation, involving a key enzyme in the pathway, pyruvate orthophosphate dikinase (PPDK, which is regulated by a bifunctional enzyme, Regulatory Protein (RP. The robust mechanism is based on avidity of the bifunctional enzyme RP to its multimeric substrate PPDK, and on a product-inhibition feedback loop that couples the system output to the activity of the bifunctional regulator. The model provides an explanation for several unusual biochemical characteristics of the system and predicts that the system's output, phosphoenolpyruvate (PEP formation rate, is insensitive to fluctuations in enzyme levels (PPDK and RP, substrate levels (ATP and pyruvate and the catalytic rate of PPDK, while remaining sensitive to the system's input (light levels. Conclusions The presented PPDK mechanism is a new way to achieve robustness using product inhibition as a feedback loop on a bifunctional regulatory enzyme. This mechanism exhibits robustness to protein and metabolite levels as well as to catalytic rate changes. At the same time, the output of the system remains tuned to input levels.

Supramolecular structure of enterobacterial wild-type lipopolysaccharides (LPS), fractions thereof, and their neutralization by Pep19-2.5.

Science.gov (United States)

Brandenburg, Klaus; Heinbockel, Lena; Correa, Wilmar; Fukuoka, Satoshi; Gutsmann, Thomas; Zähringer, Ulrich; Koch, Michel H J

2016-04-01

Lipopolysaccharides (LPS) belong to the strongest immune-modulating compounds known in nature, and are often described as pathogen-associated molecular patterns (PAMPs). In particular, at higher concentrations they are responsible for sepsis and the septic shock syndrome associated with high lethality. Since most data are indicative that LPS aggregates are the bioactive units, their supramolecular structures are considered to be of outmost relevance for deciphering the molecular mechanisms of its bioactivity. So far, however, most of the data available addressing this issue, were published only for the lipid part (lipid A) and the core-oligosaccharide containing rough LPS, representing the bioactive unit. By contrast, it is well known that most of the LPS specimen identified in natural habitats contain the smooth-form (S-form) LPS, which carry additionally a high-molecular polysaccharide (O-chain). To fill this lacuna and going into a more natural system, here various wild-type (smooth form) LPS including also some LPS fractions were investigated by small-angle X-ray scattering with synchrotron radiation to analyze their aggregate structure. Furthermore, the influence of a recently designed synthetic anti-LPS peptide (SALP) Pep19-2.5 on the aggregate structure, on the binding thermodynamics, and on the cytokine-inducing activity of LPS were characterized, showing defined aggregate changes, high affinity binding and inhibition of cytokine secretion. The data obtained are suitable to refine our view on the preferences of LPS for non-lamellar structures, representing the highest bioactive forms which can be significantly influenced by the binding with neutralizing peptides such as Pep19-2.5. Copyright © 2016 Elsevier Inc. All rights reserved.

Report on the International Society for Laboratory Hematology Survey on guidelines to support clinical hematology laboratory practice.

Science.gov (United States)

Hayward, C P M; Moffat, K A; George, T I; Proytcheva, M; Iorio, A

2016-05-01

Given the importance of evidence-based guidelines in health care, we surveyed the laboratory hematology community to determine their opinions on guideline development and their experience and interest in developing clinical hematology laboratory practice guidelines. The study was conducted using an online survey, distributed to members of the International Society for Laboratory Hematology (ISLH) in 2015, with analysis of collected, anonymized responses. A total of 245 individuals participated. Most worked in clinical and/or research laboratories (83%) or industry (11%). 42% felt there were gaps in current guidelines. The majority (58%) recommended that ISLH engages its membership in guideline development. Participants differed in their familiarity with, and use of, different organizations' guidelines. Participants felt it was important to follow best practice recommendations on guideline development, including engagement of experts, statement about conflict of interests and how they were managed, systematic review and grading evidence for recommendations, identifying recommendations lacking evidence or consensus, and public input and peer review of the guideline. Moreover, it was considered important to provide guidelines free of charge. Industry involvement in guidelines was considered less important. The clinical laboratory hematology community has high expectations of laboratory practice guidelines that are consistent with recent recommendations on evidence-based guideline development. © 2016 John Wiley & Sons Ltd.

Aircraft Fire Protection Laboratory

Data.gov (United States)

Federal Laboratory Consortium — The Navy Aircraft Protection Laboratory provides complete test support for all Navy air vehicle fire protection systems.The facility allows for the simulation of a...

On the Viability of Supporting Institutional Sharing of Remote Laboratory Facilities

Science.gov (United States)

Lowe, David; Dang, Bridgette; Daniel, Keith; Murray, Stephen; Lindsay, Euan

2015-01-01

Laboratories are generally regarded as critical to engineering education, and yet educational institutions face significant challenges in developing and maintaining high-quality laboratory facilities. Remote laboratories are increasingly being explored as a partial solution to this challenge, with research showing that--for the right learning…

Test results of pre-production prototype distributed ion pump design for the PEP-II Asymmetric B-Factory collider

Energy Technology Data Exchange (ETDEWEB)

Holdener, F.R.; Behne, D.; Hathaway, D. [and others

1995-04-24

We have built and tested a plate-type pre-production distributed Ion Pump (DIP) for the PEP-II B-Factory High Energy Ring (HER). The design has been an earlier design to use less materials and to costs. Penning cell hole sizes of 15, 18, and 21 mm have been tested in a uniform magnetic field of 0.18 T to optimize pumping speed. The resulting final DIP design consisting of a 7-plate, 15 mm basic cell size anode was magnetic field of the HER dipole. A description of the final optimized DIP design will be presented along with the test results of the pumping speed measurements.

Good Laboratory Practice. Part 3. Implementing Good Laboratory Practice in the Analytical Lab

Science.gov (United States)

Wedlich, Richard C.; Pires, Amanda; Fazzino, Lisa; Fransen, Joseph M.

2013-01-01

Laboratories submitting experimental results to the Food and Drug Administration (FDA) or the Environmental Protection Agency (EPA) in support of Good Laboratory Practice (GLP) nonclinical laboratory studies must conduct such work in compliance with the GLP regulations. To consistently meet these requirements, lab managers employ a "divide…

Prescription of Non-Occupational Post-Exposure HIV Prophylaxis by Emergency Physicians: An Analysis on Accuracy of Prescription and Compliance.

Directory of Open Access Journals (Sweden)

Stefano Malinverni

Full Text Available We conducted a retrospective analysis of data from a prospective nPEP (non-Occupational Post Exposure Prophylaxis registry based on patients consulting at one academic Emergency department located in Brussels, Belgium. We review here 1,357 cases consulting from January 2011 to December 2013.The objective of the study is to determine whether emergency physicians prescribe nPEP according to national guideline with support from IDS (infectious disease specialists. As this intervention has a high cost we wanted to verify correct allocation of treatment to high risk patients. Moreover we wanted to determine whether compliance to nPEP when prescribed by an Emergency Physician was different from literature reports. Finally we wanted to describe the population consulting for nPEP at our center. Emergency physicians prescribed nPEP more frequently in high risk exposures (98.6% compared to intermediate risk exposures (53.2%; adequately allocating resources from a public health perspective. Appropriateness of prescription when evaluated according to nPEP Belgian guidelines was 98.8%.Compliance with nPEP prescribed by Emergency physicians was 60% in our study. Compliance was the highest in MSM (Men who have Sex with Men while sexual assault victims showed the lowest compliance. Altogether this study suggests that Emergency physicians can safely and adequately prescribe nPEP when supported by a comprehensive guideline. Recognizing intrinsic differences within heterogeneous populations consulting for nPEP may improve compliance to this high-cost public health intervention.

Calgary Laboratory Services

Directory of Open Access Journals (Sweden)

James R. Wright MD, PhD

2015-12-01

Full Text Available Calgary Laboratory Services provides global hospital and community laboratory services for Calgary and surrounding areas (population 1.4 million and global academic support for the University of Calgary Cumming School of Medicine. It developed rapidly after the Alberta Provincial Government implemented an austerity program to address rising health care costs and to address Alberta’s debt and deficit in 1994. Over roughly the next year, all hospital and community laboratory test funding within the province was put into a single budget, fee codes for fee-for-service test billing were closed, roughly 40% of the provincial laboratory budget was cut, and roughly 40% of the pathologists left the province of Alberta. In Calgary, in the face of these abrupt changes in the laboratory environment, private laboratories, publicly funded hospital laboratories and the medical school department precipitously and reluctantly merged in 1996. The origin of Calgary Laboratory Services was likened to an “unhappy shotgun marriage” by all parties. Although such a structure could save money by eliminating duplicated services and excess capacity and could provide excellent city-wide clinical service by increasing standardization, it was less clear whether it could provide strong academic support for a medical school. Over the past decade, iterations of the Calgary Laboratory Services model have been implemented or are being considered in other Canadian jurisdictions. This case study analyzes the evolution of Calgary Laboratory Services, provides a metric-based review of academic performance over time, and demonstrates that this model, essentially arising as an unplanned experiment, has merit within a Canadian health care context.

A measurement evaluation program to support nuclear material control and accountability measurements in Brazilian laboratories

International Nuclear Information System (INIS)

Dias, Fabio C.; Mason, Peter

2013-01-01

A measurement evaluation program (MEP) is one of a number of valuable tools that analytical chemists can use to ensure that the data produced in the laboratory are fit for their intended purpose and consistent with expected performance values at a given time. As such, participation in a MEP is an important indicator of the quality of analytical data, and is recognized as such by independent regulatory and/or accreditation bodies. With the intent to implement such a program in Brazil, in November 2012 the Nuclear Energy Commission of Brazil (CNEN), with support from the Department of Energy of the United States' (US-DOE International Safeguards and Engagement Program), decided to initiate a technical cooperation project aiming at organizing a Safeguards Measurement Evaluation Program (SMEP) for Brazilian facilities. The project, entitled Action Sheet 23, was formalized under the terms of the Agreement between the US-DOE and the CNEN concerning research and development in nuclear material control, accountancy, verification, physical protection, and advanced containment and surveillance technologies for International Safeguards Applications. The work, jointly performed by the CNEN's Safeguards Laboratory (LASAL) and the New Brunswick Laboratory (NBL), has the objective to strengthen the traceability of accountability measurements and ensure adequate quality of safeguards measurements for facilities within Brazil, utilizing test samples characterized and provided by NBL. Recommendations to participants included measurement frequency, number of results per sample and format for reporting results using ISO methods for calculating and expressing measurement uncertainties. In this paper, we discuss the main steps taken by CNEN and NBL aiming at implementing such a program and the expected results, in particular the impact of uncertainty estimation on the evaluation of performance of each participant laboratory. The program is considered by Brazilian safeguards authorities

A measurement evaluation program to support nuclear material control and accountability measurements in Brazilian laboratories

Energy Technology Data Exchange (ETDEWEB)

Dias, Fabio C., E-mail: fabio@ird.gov.br [Comissao Nacional de Energia Nuclear (CNEN-RJ), Rio de Janeiro, RJ (Brazil); Mason, Peter, E-mail: peter.mason@ch.doe.gov [New Brunswick Laboratory (DOE/NBL), Argonne, IL (United States)

2013-07-01

A measurement evaluation program (MEP) is one of a number of valuable tools that analytical chemists can use to ensure that the data produced in the laboratory are fit for their intended purpose and consistent with expected performance values at a given time. As such, participation in a MEP is an important indicator of the quality of analytical data, and is recognized as such by independent regulatory and/or accreditation bodies. With the intent to implement such a program in Brazil, in November 2012 the Nuclear Energy Commission of Brazil (CNEN), with support from the Department of Energy of the United States' (US-DOE International Safeguards and Engagement Program), decided to initiate a technical cooperation project aiming at organizing a Safeguards Measurement Evaluation Program (SMEP) for Brazilian facilities. The project, entitled Action Sheet 23, was formalized under the terms of the Agreement between the US-DOE and the CNEN concerning research and development in nuclear material control, accountancy, verification, physical protection, and advanced containment and surveillance technologies for International Safeguards Applications. The work, jointly performed by the CNEN's Safeguards Laboratory (LASAL) and the New Brunswick Laboratory (NBL), has the objective to strengthen the traceability of accountability measurements and ensure adequate quality of safeguards measurements for facilities within Brazil, utilizing test samples characterized and provided by NBL. Recommendations to participants included measurement frequency, number of results per sample and format for reporting results using ISO methods for calculating and expressing measurement uncertainties. In this paper, we discuss the main steps taken by CNEN and NBL aiming at implementing such a program and the expected results, in particular the impact of uncertainty estimation on the evaluation of performance of each participant laboratory. The program is considered by Brazilian safeguards

Test results of distributed ion pump designs for the PEP-II Asymmetric B-Factory collider

Energy Technology Data Exchange (ETDEWEB)

Calderon, M.; Holdener, F.; Peterson, D. [Lawrence Livermore National Lab., CA (United States)] [and others

1994-07-01

The testing facility measurement methods and results of prototype distributed ion pump (DIP) designs for the PEP-II B-Factory High Energy Ring are presented. Two basic designs with 5- or 7-anode plates were tested at LLNL with penning cell sizes of 15, 18, and 21 mm. Direct comparison of 5- and 7-plate anodes with 18 mm holes shows increased pumping speed with the 7-plate design. The 5-plate, 18 mm and 7-plate, 15 mm designs both gave an average pumping speed of 135 1/s/m at 1 {times} 10{sup {minus}8} Torr nitrogen base pressure in a varying 0.18 T peak B-field. Comparison of the three hole sizes indicates that cells smaller than the 15 mm tested can be efficiently used to obtain higher pumping speeds for the same anode plate sizes used.

Test results of distributed ion pump designs for the PEP-II Asymmetric B-Factory collider

International Nuclear Information System (INIS)

Calderon, M.; Holdener, F.; Peterson, D.

1994-07-01

The testing facility measurement methods and results of prototype distributed ion pump (DIP) designs for the PEP-II B-Factory High Energy Ring are presented. Two basic designs with 5- or 7-anode plates were tested at LLNL with penning cell sizes of 15, 18, and 21 mm. Direct comparison of 5- and 7-plate anodes with 18 mm holes shows increased pumping speed with the 7-plate design. The 5-plate, 18 mm and 7-plate, 15 mm designs both gave an average pumping speed of 135 1/s/m at 1 x 10 -8 Torr nitrogen base pressure in a varying 0.18 T peak B-field. Comparison of the three hole sizes indicates that cells smaller than the 15 mm tested can be efficiently used to obtain higher pumping speeds for the same anode plate sizes used

Vacuum system of the high energy ring of an asymmetric B-factory based on PEP

International Nuclear Information System (INIS)

Barletta, W.A.; Calderon, M.O.; Wong, R.; Jenkins, T.M.

1991-01-01

The multi-ampere currents required for high luminosity operation of an asymmetric B factory leads to extremely stressing requirements on a vacuum system suitable for maintaining long beam-gas lifetimes and acceptable background levels in the detector. We present the design for a Cu alloy vacuum chamber and its associated pumping system for the 9 GeV electron storage ring of the proposed B factory based on PEP. The excellent thermal and photo-desorption properties of Cu allows handling the high proton flux in a conventional, single chamber design with distributed ion pumps. The x-ray opacity of the Cu is sufficiently high that no additional lead shielding is necessary to protect the dipoles from the intense synchrotron radiation generated by the beam. The design allows chamber commissioning in <500 hr of operation. 5 refs., 3 figs., 2 tabs

94-1 Research and development project lead laboratory support. Status report, July 1, 1996--September 30, 1996

Energy Technology Data Exchange (ETDEWEB)

Rink, N. [comp.

1997-03-01

This document reports status and technical progress for Los Alamos 94-1 Research and Development projects. Updated schedule charts are shown in the appendix. This is the fourth status report published for Los Alamos National Laboratory 94-1 Research and Development Project Support. The Department of Energy Office of Environmental Management (DOE/EM) funds these projects in order to support the storage or disposal of legacy plutonium and plutonium-bearing materials resulting from weapons production throughout the DOE complex. This document also serves as an end-for-year review of projects and positions the program for FY97.

Analytical Laboratory

Data.gov (United States)

Federal Laboratory Consortium — The Analytical Labspecializes in Oil and Hydraulic Fluid Analysis, Identification of Unknown Materials, Engineering Investigations, Qualification Testing (to support...

Development of Biodiversity Laboratory to Support the Establishment of Flora and Fauna Database in the Vicinity of Nuclear Facility

International Nuclear Information System (INIS)

Nor Humaira Lau Abdullah; Anis Nadia Mohd Faisol Mahadeven; Mohd Noor Hidayat Adenan

2015-01-01

The Biodiversity Laboratory (44128) at Agrotechnology and Biosciences Division (BAB) was developed using One-Off 2014 budget. The renovation works of Seed Technology Laboratory into Biodiversity Laboratory was planned in 2013 and was fully completed in early 2015. This laboratory serves as a centre for development and storage of flora and fauna database. Thus far, this laboratory houses various facilities that befit the function of this laboratory, such as small mammalian and insects sampling tools, herbarium specimen preparation tools, fume chamber, and work benches. Among the activities carried out in this laboratory were sampling and processing of flora, fauna and mushroom specimens collected in the vicinity of nuclear facility besides exhibiting processed/preserved herbaria, mushrooms, fauna and insects specimens. On the other hand, activities planned include cataloguing of existing specimens, online database development, study on ionising radiation towards development of bio indicator, and development of Standard Operating Procedure (SOP). However there are some limitations in terms of tools (supercomputer, camera microscope, photography set-up and drying oven) and not to mention, expertise. In order to overcome the limitations, some recommendations for improvement can be considered for instance fund application, hiring staffs in desired field of expertise (botanist and zoologist) and training's. In summary, this laboratory has potential to support the aspiration of Nuclear Malaysia to be a TSO for national nuclear power development plan in the aspect of environmental and ecosystem protection especially towards non-human biota. (author)

Progress on the superconducting magnet for the time projection chamber experiment (TPC) at PEP

International Nuclear Information System (INIS)

Green, M.A.; Eberhard, P.H.; Burns, W.A.

1980-01-01

The TPC (Time Projection Chamber) experiment at PEP will have a two meter inside diameter superconducting magnet which creatests a 1.5 T uniform solenoidal field for the TPC. The superconducting magnet coil, cryostat, cooling system, and the TPC gas pressure vessel (which operatests at 11 atm) were designed to be about two thirds of a radiation length thick. As a result, a high current density coil design was chosen. The magnet is cooled by forced flow two phase helium. The TPC magnet is the largest adiabatically stable superconducting magnet built to date. The paper presents the parameters of the TPC thin solenoid and its subsystems. Tests results from the Spring 1980 cryogenic tes are presented. The topics to be dealt with in the paper are cryogenic services and the tests of magnet subsystems such as the folded current leads. Large thin superconducting magnet technology will be important to large detectors to be used on LEP

Measurements of higher-order mode damping in the PEP-II low-power test cavity

International Nuclear Information System (INIS)

Rimmer, R.A.; Goldberg, D.A.

1993-05-01

The paper describes the results of measurements of the Higher-Order Mode (HOM) spectrum of the low-power test model of the PEP-II RF cavity and the reduction in the Q's of the modes achieved by the addition of dedicated damping waveguides. All the longitudinal (monopole) and deflecting (dipole) modes below the beam pipe cut-off are identified by comparing their measured frequencies and field distributions with calculations using the URMEL code. Field configurations were determined using a perturbation method with an automated bead positioning system. The loaded Q's agree well with the calculated values reported previously, and the strongest HOMs are damped by more than three orders of magnitude. This is sufficient to reduce the coupled-bunch growth rates to within the capability of a reasonable feedback system. A high power test cavity will now be built to validate the thermal design at the 150 kW nominal operating level, as described elsewhere at this conference

ESSReS-PEP, an international and interdisciplinary postgraduate education concept on Earth and Environmental Sciences

Science.gov (United States)

Grosfeld, Klaus; Lohmann, Gerrit; Ladstätter-Weißenmayer, Annette; Burrows, John

2013-04-01

Promoting young researchers is a major priority of the German Helmholtz Association. Since more than five years graduate and postgraduate education in the field of Earth System and Environmental Science has been established in Bremen and Bremerhaven, north-western Germany. Using the network and collaboration of experts and specialists on observational and paleoclimate data as well as on statistical data analysis and climate modelling from two Universities and the Helmholtz research institute on Polar and Marine Research, master and PhD students are trained to understand, decipher and cope with the challenges of recent climate change on an highly interdisciplinary and inter-institutional level. The existing research infrastructure at the Alfred Wegener Institute in Bremerhaven (AWI), University of Bremen, and Jacobs University Bremen offers a unique research environment to study past, present and future changes of the climate system, with special focus on high latitudinal processes. It covers all kind of disciplines, climate science, geosciences and biosciences, and provides a consistent framework for education and qualification of a new generation of expertly trained, internationally competitive master and PhD students. On postgraduate level, the Postgraduate Programme Environmental Physics (PEP) at the University of Bremen (www.pep.uni-bremen.de) educates the participants on the complex relationship between atmosphere, hydrosphere (ocean), cryosphere (ice region) and solid earth (land). Here, the learning of experimental methods in environmental physics at the most advanced level, numerical data analysis using supercomputers, and data interpretation via sophisticated methods prepare students for a scientific career. Within cooperation with the Ocean University of China (OUC) students are participating one year in the PEP programme during their master studies since 2006, to get finally a double degree of both universities. At the Alfred Wegener Institute for Polar

Presentation of child sexual abuse cases to Queen Elizabeth ...

African Journals Online (AJOL)

presenting to Queen Elizabeth Central Hospital (QECH),. Blantyre ... American guidelines recommend HIV PEP for children after ..... reactions, poor social support and traumatic associations ... However animal models of PEP have shown.