Human β satellite DNA: Genomic organization and sequence definition of a class of highly repetitive tandem DNA

International Nuclear Information System (INIS)

Waye, J.S.; Willard, H.F.

1989-01-01

The authors describe a class of human repetitive DNA, called β satellite, that, at a most fundamental level, exists as tandem arrays of diverged ∼68-base-pair monomer repeat units. The monomer units are organized as distinct subsets, each characterized by a multimeric higher-order repeat unit that is tandemly reiterated and represents a recent unit of amplification. They have cloned, characterized, and determined the sequence of two β satellite higher-order repeat units: one located on chromosome 9, the other on the acrocentric chromosomes (13, 14, 15, 21, and 22) and perhaps other sites in the genome. Analysis by pulsed-field gel electrophoresis reveals that these tandem arrays are localized in large domains that are marked by restriction fragment length polymorphisms. In total, β-satellite sequences comprise several million base pairs of DNA in the human genome. Analysis of this DNA family should permit insights into the nature of chromosome-specific and nonspecific modes of satellite DNA evolution and provide useful tools for probing the molecular organization and concerted evolution of the acrocentric chromosomes

Extrachromosomal circles of satellite repeats and 5S ribosomal DNA in human cells

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Cohen Sarit

2010-03-01

Full Text Available Abstract Background Extrachomosomal circular DNA (eccDNA is ubiquitous in eukaryotic organisms and was detected in every organism tested, including in humans. A two-dimensional gel electrophoresis facilitates the detection of eccDNA in preparations of genomic DNA. Using this technique we have previously demonstrated that most of eccDNA consists of exact multiples of chromosomal tandemly repeated DNA, including both coding genes and satellite DNA. Results Here we report the occurrence of eccDNA in every tested human cell line. It has heterogeneous mass ranging from less than 2 kb to over 20 kb. We describe eccDNA homologous to human alpha satellite and the SstI mega satellite. Moreover, we show, for the first time, circular multimers of the human 5S ribosomal DNA (rDNA, similar to previous findings in Drosophila and plants. We further demonstrate structures that correspond to intermediates of rolling circle replication, which emerge from the circular multimers of 5S rDNA and SstI satellite. Conclusions These findings, and previous reports, support the general notion that every chromosomal tandem repeat is prone to generate eccDNA in eukryoric organisms including humans. They suggest the possible involvement of eccDNA in the length variability observed in arrays of tandem repeats. The implications of eccDNA on genome biology may include mechanisms of centromere evolution, concerted evolution and homogenization of tandem repeats and genomic plasticity.

Innovation and marketing strategies for PDO products: the case of “Parmigiano Reggiano” as an ingredient

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Maria Cecilia Mancini

2017-01-01

Full Text Available Typical products can contribute to socio-economic development of their place of origin if they are able to take part in the logic of the global market. The aim of this research is to examine one type of innovation, the use of PDO products as ingredients, as a part of a strategy to re-launch PDO products which have a mature market. The evolution of the concept of innovation is discussed; innovation is then considered in relation to product life cycle and information asymmetry. There is then a case study on the use of PDO Parmigiano Reggiano cheese as an ingredient in industrial processing. This case shows that even incremental innovation can have serious effects for the market when it is applied on production phases which lie outside the direct control of the PDO producers. In order to protect the consumer as well as the PDO producer, it would be advisable for new legislation to regulate in more detail innovation involving products bearing origin certificates.

Characterization and authentication of Spanish PDO wine vinegars using multidimensional fluorescence and chemometrics

DEFF Research Database (Denmark)

Ríos-Reina, Rocío; Elcoroaristizabal, Saioa; Ocaña-Gonzalez, Juan A.

2017-01-01

This work assesses the potential of multidimensional fluorescence spectroscopy combined with chemometrics for characterization and authentication of Spanish Protected Designation of Origin (PDO) wine vinegars. Seventy-nine vinegars of different categories (aged and sweet) belonging to the Spanish...... obtained better results (>92% of classification). In each category, SVM also allows the differentiation between PDOs. The proposed methodology could be used as an analysis method for the authentication of Spanish PDO wine vinegars....

Satellite DNA Sequences in Canidae and Their Chromosome Distribution in Dog and Red Fox.

Science.gov (United States)

Vozdova, Miluse; Kubickova, Svatava; Cernohorska, Halina; Fröhlich, Jan; Rubes, Jiri

2016-01-01

Satellite DNA is a characteristic component of mammalian centromeric heterochromatin, and a comparative analysis of its evolutionary dynamics can be used for phylogenetic studies. We analysed satellite and satellite-like DNA sequences available in NCBI for 4 species of the family Canidae (red fox, Vulpes vulpes, VVU; domestic dog, Canis familiaris, CFA; arctic fox, Vulpes lagopus, VLA; raccoon dog, Nyctereutes procyonoides procyonoides, NPR) by comparative sequence analysis, which revealed 86-90% intraspecies and 76-79% interspecies similarity. Comparative fluorescence in situ hybridisation in the red fox and dog showed signals of the red fox satellite probe in canine and vulpine autosomal centromeres, on VVUY, B chromosomes, and in the distal parts of VVU9q and VVU10p which were shown to contain nucleolus organiser regions. The CFA satellite probe stained autosomal centromeres only in the dog. The CFA satellite-like DNA did not show any significant sequence similarity with the satellite DNA of any species analysed and was localised to the centromeres of 9 canine chromosome pairs. No significant heterochromatin block was detected on the B chromosomes of the red fox. Our results show extensive heterogeneity of satellite sequences among Canidae and prove close evolutionary relationships between the red and arctic fox. © 2017 S. Karger AG, Basel.

A mechanistic study of hydrogen gas sensing by PdO nanoflake thin films at temperatures below 250 °C.

Science.gov (United States)

Chiang, Yu-Ju; Li, Kuang-Chung; Lin, Yi-Chieh; Pan, Fu-Ming

2015-02-07

We prepared PdO nanoflake thin films on the SiO2 substrate by reactive sputter deposition, and studied their sensing response to H2 at temperatures between 25 and 250 °C. In addition to the oxygen ionosorption model, which is used to describe the early H2 sensing response over the temperature range studied, the H2 sensing kinetics of the PdO thin films can be separated into three temperature regimes: temperatures below 100 °C, around 150 °C and above 200 °C. At temperatures below 100 °C, PdO reduction is the dominant reaction affecting the H2 sensing behavior. At temperatures around 150 °C, Pd reoxidation kinetically competes with PdO reduction leading to a complicated sensing characteristic. Active PdO reduction by H2 promotes the continuing growth of Pd nanoislands, facilitating dissociative oxygen adsorption and thus the subsequent Pd reoxidation in the H2-dry air gas mixture. The kinetic competition between the PdO reduction and reoxidation at 150 °C leads to the observation of an inverse of the increase in the sensor conductivity. At temperatures above 200 °C, the PdO sensor exhibits a sensor signal monotonically increasing with the H2 concentration, and the H2 sensing behavior is consistent with the Mars-van-Krevelen redox mechanism.

Spontaneous dispersion of PdO onto acid sites of zeolites studied by in situ DXAFS

CERN Document Server

Okumura, K; Niwa, M; Yokota, S; Kato, K; Tanida, H; Uruga, T

2003-01-01

The generation of highly dispersed PdO over zeolite supports was studied using in situ energy-dispersive XAFS (DXAFS) technique. From the comparison with the Na-ZSM-5, it was found that the oxidation as well as the spontaneous dispersion of Pd was promoted through the interaction between PdO and acid sites of H-form zeolites. (author)

Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses

International Nuclear Information System (INIS)

Briddon, Rob W.; Bull, Simon E.; Amin, Imran; Idris, Ali M.; Mansoor, Shahid; Bedford, Ian D.; Dhawan, Poonam; Rishi, Narayan; Siwatch, Surender S.; Abdel-Salam, Aly M.; Brown, Judith K.; Zafar, Yusuf; Markham, Peter G.

2003-01-01

DNA β molecules are symptom-modulating, single-stranded DNA satellites associated with monopartite begomoviruses (family Geminiviridae). Such molecules have thus far been shown to be associated with Ageratum yellow vein virus from Singapore and Cotton leaf curl Multan virus from Pakistan. Here, 26 additional DNA β molecules, associated with diverse plant species obtained from different geographical locations, were cloned and sequenced. These molecules were shown to be widespread in the Old World, where monopartite begomoviruses are known to occur. Analysis of the sequences revealed a highly conserved organization for DNA β molecules consisting of a single conserved open reading frame, an adenine-rich region, and a region of high sequence conservation [the satellite conserved region (SCR)]. The SCR contains a potential hairpin structure with the loop sequence TAA/GTATTAC; similar to the origins of replication of geminiviruses and nanoviruses. Two major groups of DNA β satellites were resolved by phylogenetic analyses. One group originated from hosts within the Malvaceae and the second from a more diverse group of plants within the Solanaceae and Compositae. Within the two clusters, DNA β molecules showed relatedness based both on host and geographic origin. These findings strongly support coadaptation of DNA β molecules with their respective helper begomoviruses

Comparative Analysis of Satellite DNA in the Drosophila melanogaster Species Complex

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Madhav Jagannathan

2017-02-01

Full Text Available Satellite DNAs are highly repetitive sequences that account for the majority of constitutive heterochromatin in many eukaryotic genomes. It is widely recognized that sequences and locations of satellite DNAs are highly divergent even in closely related species, contributing to the hypothesis that satellite DNA differences may underlie speciation. However, due to its repetitive nature, the mapping of satellite DNAs has been mostly left out of recent genomics analyses, hampering the use of molecular genetics techniques to better understand their role in speciation and evolution. Satellite DNAs are most extensively and comprehensively mapped in Drosophila melanogaster, a species that is also an excellent model system with which to study speciation. Yet the lack of comprehensive knowledge regarding satellite DNA identity and location in its sibling species (D. simulans, D. mauritiana, and D. sechellia has prevented the full utilization of D. melanogaster in studying speciation. To overcome this problem, we initiated the mapping of satellite DNAs on the genomes of the D. melanogaster species complex (D. melanogaster, D. simulans, D. mauritiana, and D. sechellia using multi-color fluorescent in situ hybridization (FISH probes. Our study confirms a striking divergence of satellite DNAs in the D. melanogaster species complex, even among the closely related species of the D. simulans clade (D. simulans, D. mauritiana, and D. sechellia, and suggests the presence of unidentified satellite sequences in these species.

Distribution of DTHS3 satellite DNA across 12 bivalve species Eva ...

Indian Academy of Sciences (India)

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In this work, characterization of DTHS3 satellite DNA was further expanded within the Class. Bivalvia. Monomer variants of DTHS3 satDNA were compared in 12 bivalve species belonging to two different Subclasses, Heterodonta and Pteriomorphia. This satDNA, whose age is estimated to a minimum of 516 Ma, ...

TAREAN: a computational tool for identification and characterization of satellite DNA from unassembled short reads.

Science.gov (United States)

Novák, Petr; Ávila Robledillo, Laura; Koblížková, Andrea; Vrbová, Iva; Neumann, Pavel; Macas, Jirí

2017-07-07

Satellite DNA is one of the major classes of repetitive DNA, characterized by tandemly arranged repeat copies that form contiguous arrays up to megabases in length. This type of genomic organization makes satellite DNA difficult to assemble, which hampers characterization of satellite sequences by computational analysis of genomic contigs. Here, we present tandem repeat analyzer (TAREAN), a novel computational pipeline that circumvents this problem by detecting satellite repeats directly from unassembled short reads. The pipeline first employs graph-based sequence clustering to identify groups of reads that represent repetitive elements. Putative satellite repeats are subsequently detected by the presence of circular structures in their cluster graphs. Consensus sequences of repeat monomers are then reconstructed from the most frequent k-mers obtained by decomposing read sequences from corresponding clusters. The pipeline performance was successfully validated by analyzing low-pass genome sequencing data from five plant species where satellite DNA was previously experimentally characterized. Moreover, novel satellite repeats were predicted for the genome of Vicia faba and three of these repeats were verified by detecting their sequences on metaphase chromosomes using fluorescence in situ hybridization. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Integration of hepatitis B virus DNA in chromosome-specific satellite sequences

International Nuclear Information System (INIS)

Shaul, Y.; Garcia, P.D.; Schonberg, S.; Rutter, W.J.

1986-01-01

The authors previously reported the cloning and detailed analysis of the integrated hepatitis B virus sequences in a human hepatoma cell line. They report here the integration of at least one of hepatitis B virus at human satellite DNA sequences. The majority of the cellular sequences identified by this satellite were organized as a multimeric composition of a 0.6-kilobase EcoRI fragment. This clone hybridized in situ almost exclusively to the centromeric heterochromatin of chromosomes 1 and 16 and to a lower extent to chromosome 2 and to the heterochromatic region of the Y chromosome. The immediate flanking host sequence appeared as a hierarchy of repeating units which were almost identical to a previously reported human satellite III DNA sequence

Supervised chemical pattern recognition in almond ( Prunus dulcis ) Portuguese PDO cultivars: PCA- and LDA-based triennial study.

Science.gov (United States)

Barreira, João C M; Casal, Susana; Ferreira, Isabel C F R; Peres, António M; Pereira, José Alberto; Oliveira, M Beatriz P P

2012-09-26

Almonds harvested in three years in Trás-os-Montes (Portugal) were characterized to find differences among Protected Designation of Origin (PDO) Amêndoa Douro and commercial non-PDO cultivars. Nutritional parameters, fiber (neutral and acid detergent fibers, acid detergent lignin, and cellulose), fatty acids, triacylglycerols (TAG), and tocopherols were evaluated. Fat was the major component, followed by carbohydrates, protein, and moisture. Fatty acids were mostly detected as monounsaturated and polyunsaturated forms, with relevance of oleic and linoleic acids. Accordingly, 1,2,3-trioleoylglycerol and 1,2-dioleoyl-3-linoleoylglycerol were the major TAG. α-Tocopherol was the leading tocopherol. To verify statistical differences among PDO and non-PDO cultivars independent of the harvest year, data were analyzed through an analysis of variance, a principal component analysis, and a linear discriminant analysis (LDA). These differences identified classification parameters, providing an important tool for authenticity purposes. The best results were achieved with TAG analysis coupled with LDA, which proved its effectiveness to discriminate almond cultivars.

Traceability of PDO Olive Oil “Terra di Bari” Using High Resolution Melting

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Cinzia Montemurro

2015-01-01

Full Text Available The aim of the research was to verify the applicability of microsatellite (SSR markers in High Resolution Melting (HRM analysis for the identification of the olive cultivars used in the “Terra di Bari” PDO extra virgin olive oil. A panel of nine cultivars, widespread in Apulia region, was tested with seventeen SSR primer pairs and the PCR products were at first analysed with a Genetic Analyzer automatic sequencer. An identification key was obtained for the nine cultivars, which showed an unambiguous discrimination among the varieties constituting the “Terra di Bari” PDO extra virgin olive oil: Cima di Bitonto, Coratina, and Ogliarola. Subsequently, an SSR based method was set up with the DCA18 marker, coupled with HRM analysis for the distinction of the Terra di Bari olive oil from non-Terra di Bari olive oil using different mixtures. Thus, this analysis enabled the distinction and identification of the PDO mixtures. Hence, this assay provided a flexible, cost-effective, and closed-tube microsatellite genotyping method, well suited to varietal identification and authentication analysis in olive oil.

RNA Pol II promotes transcription of centromeric satellite DNA in beetles.

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Zeljka Pezer

Full Text Available Transcripts of centromeric satellite DNAs are known to play a role in heterochromatin formation as well as in establishment of the kinetochore. However, little is known about basic mechanisms of satellite DNA expression within constitutive heterochromatin and its regulation. Here we present comprehensive analysis of transcription of abundant centromeric satellite DNA, PRAT from beetle Palorus ratzeburgii (Coleoptera. This satellite is characterized by preservation and extreme sequence conservation among evolutionarily distant insect species. PRAT is expressed in all three developmental stages: larvae, pupae and adults at similar level. Transcripts are abundant comprising 0.033% of total RNA and are heterogeneous in size ranging from 0.5 kb up to more than 5 kb. Transcription proceeds from both strands but with 10 fold different expression intensity and transcripts are not processed into siRNAs. Most of the transcripts (80% are not polyadenylated and remain in the nucleus while a small portion is exported to the cytoplasm. Multiple, irregularly distributed transcription initiation sites as well as termination sites have been mapped within the PRAT sequence using primer extension and RLM-RACE. The presence of cap structure as well as poly(A tails in a portion of the transcripts indicate RNA polymerase II-dependent transcription and a putative polymerase II promoter site overlaps the most conserved part of the PRAT sequence. The treatment of larvae with alpha-amanitin decreases the level of PRAT transcripts at concentrations that selectively inhibit pol II activity. In conclusion, stable, RNA polymerase II dependant transcripts of abundant centromeric satellite DNA, not regulated by RNAi, have been identified and characterized. This study offers a basic understanding of expression of highly abundant heterochromatic DNA which in beetle species constitutes up to 50% of the genome.

Artificial nose, NIR and UV-visible spectroscopy for the characterisation of the PDO Chianti Classico olive oil.

Science.gov (United States)

Forina, M; Oliveri, P; Bagnasco, L; Simonetti, R; Casolino, M C; Nizzi Grifi, F; Casale, M

2015-11-01

An authentication study of the Italian PDO (Protected Designation of Origin) olive oil Chianti Classico, based on artificial nose, near-infrared and UV-visible spectroscopy, with a set of samples representative of the whole Chianti Classico production area and a considerable number of samples from other Italian PDO regions was performed. The signals provided by the three analytical techniques were used both individually and jointly, after fusion of the respective variables, in order to build a model for the Chianti Classico PDO olive oil. Different signal pre-treatments were performed in order to investigate their importance and their effects in enhancing and extracting information from experimental data, correcting backgrounds or removing baseline variations. Stepwise-Linear Discriminant Analysis (STEP-LDA) was used as a feature selection technique and, afterward, Linear Discriminant Analysis (LDA) and the class-modelling technique Quadratic Discriminant Analysis-UNEQual dispersed classes (QDA-UNEQ) were applied to sub-sets of selected variables, in order to obtain efficient models capable of characterising the extra virgin olive oils produced in the Chianti Classico PDO area. Copyright © 2015 Elsevier B.V. All rights reserved.

Multidecadal change of winter cyclonic activity in the Mediterranean associated with AMO and PDO

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Veronika Nikolaevna Maslova

2017-01-01

Full Text Available The paper is devoted to analysis of the cold half-year (October to March frequency of cyclones in the Mediterranean-Black Sea region associated with the global processes in the ocean-atmosphere system - the Pacific Decadal Oscillation (PDO and Atlantic Multidecadal Oscillation (AMO. Firstly, climatic conditions in the North Atlantic-European region during positive and negative phases of these global oscillations were shown using NCEP/NCAR reanalysis data for 1948 - 2016 and data of the NASA GISS Atlas of Exratropical Strom Tracks in 1961 - 1998. Mean monthly values / anomalies (composites for equal periods of the negative and positive AMO and PDO phases were calculated and compared. The results of the study support the idea that the Pacific and Atlantic influence on the climatic (multidecadal scale is realized via change of the large-scale fields of the North Atlantic anomalies typical for the interannual scale. Then spatial distribution of the frequency of cyclones in the Mediterranean-Black Sea region was obtained using global NCEP/NCAR reanalysis data sets on 1000 hPa geopotential height in 1948 - 2013. It was shown that during the positive AMO phase, frequency of cyclones in the Mediterranean was higher by absolute values in November to March over the Tyrrhenian and Ionic Seas and lower over the Anatolian peninsula. During the negative PDO phase, frequency of cyclones over the Anatolian peninsula in January to March is significantly higher than in the positive PDO phase.

Quality assessment of buccal versus blood genomic DNA using the Affymetrix 500 K GeneChip

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Martin Lisa J

2007-11-01

Full Text Available Abstract Background With the advent of genome-wide genotyping, the utility of stored buccal brushes for DNA extraction and genotyping has been questioned. We sought to describe the genomic DNA yield and concordance between stored buccal brushes and blood samples from the same individuals in the context of Affymetrix 500 K Human GeneChip genotyping. Results Buccal cytobrushes stored for ~7 years at -80°C prior to extraction yielded sufficient double stranded DNA (dsDNA to be successfully genotyped on the Affymetrix ~262 K NspI chip, with yields between 536 and 1047 ng dsDNA. Using the BRLMM algorithm, genotyping call rates for blood samples averaged 98.4%, and for buccal samples averaged 97.8%. Matched blood samples exhibited 99.2% concordance, while matched blood and buccal samples exhibited 98.8% concordance. Conclusion Buccal cytobrushes stored long-term result in sufficient dsDNA concentrations to achieve high genotyping call rates and concordance with stored blood samples in the context of Affymetrix 500 K SNP genotyping. Thus, given high-quality collection and storage protocols, it is possible to use stored buccal cytobrush samples for genome-wide association studies.

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH

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Jaime Gosálvez

2013-02-01

Full Text Available We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH. A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL, 10 with high-grade SIL (HG-SIL, and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH).

Science.gov (United States)

Cortés-Gutiérrez, Elva I; Ortíz-Hernández, Brenda L; Dávila-Rodríguez, Martha I; Cerda-Flores, Ricardo M; Fernández, José Luis; López-Fernández, Carmen; Gosálvez, Jaime

2013-02-19

We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.

Investigating unexpected magnetism of mesoporous silica-supported Pd and PdO nanoparticles

KAUST Repository

Song, Hyon Min

2015-01-13

The synthesis and magnetic behavior of matrix-supported Pd and PdO nanoparticles (NPs) are described. Mesoporous silica with hexagonal columnal packing is selected as a template, and the impregnation method with thermal annealing is used to obtain supported Pd and PdO NPs. The heating rate and the annealing conditions determine the particle size and the phase of the NPs, with a fast heating rate of 30 °C/min producing the largest supported Pd NPs. Unusual magnetic behaviors are observed. (1) Contrary to the general belief that smaller Pd NPs or cluster size particles have higher magnetization, matrix-supported Pd NPs in this study maintain the highest magnetization with room temperature ferromagnetism when the size is the largest. (2) Twin boundaries along with stacking faults are more pronounced in these large Pd NPs and are believed to be the reason for this high magnetization. Similarly, supported PdO NPs were prepared under air conditions with different heating rates. Their phase is tetragonal (P42/mmc) with cell parameters of a = 3.050 Å and c = 5.344 Å, which are slightly larger than in the bulk phase (a = 3.03 Å, c = 5.33 Å). Faster heating rate of 30 °C/min also produces larger particles and larger magnetic hysteresis loop, although magnetization is smaller and few twin boundaries are observed compared to the supported metallic Pd NPs.

Discovery of a Regulatory Motif for Human Satellite DNA Transcription in Response to BATF2 Overexpression.

Science.gov (United States)

Bai, Xuejia; Huang, Wenqiu; Zhang, Chenguang; Niu, Jing; Ding, Wei

2016-03-01

One of the basic leucine zipper transcription factors, BATF2, has been found to suppress cancer growth and migration. However, little is known about the genes downstream of BATF2. HeLa cells were stably transfected with BATF2, then chromatin immunoprecipitation-sequencing was employed to identify the DNA motifs responsive to BATF2. Comprehensive bioinformatics analyses indicated that the most significant motif discovered as TTCCATT[CT]GATTCCATTC[AG]AT was primarily distributed among the chromosome centromere regions and mostly within human type II satellite DNA. Such motifs were able to prime the transcription of type II satellite DNA in a directional and asymmetrical manner. Consistently, satellite II transcription was up-regulated in BATF2-overexpressing cells. The present study provides insight into understanding the role of BATF2 in tumours and the importance of satellite DNA in the maintenance of genomic stability. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Distribution and sequence homogeneity of an abundant satellite DNA in the beetle, Tenebrio molitor.

Science.gov (United States)

Davis, C A; Wyatt, G R

1989-01-01

The mealworm beetle, Tenebrio molitor, contains an unusually abundant and homogeneous satellite DNA which constitutes up to 60% of its genome. The satellite DNA is shown to be present in all of the chromosomes by in situ hybridization. 18 dimers of the repeat unit were cloned and sequenced. The consensus sequence is 142 nt long and lacks any internal repeat structure. Monomers of the sequence are very similar, showing on average a 2% divergence from the calculated consensus. Variant nucleotides are scattered randomly throughout the sequence although some variants are more common than others. Neighboring repeat units are no more alike than randomly chosen ones. The results suggest that some mechanism, perhaps gene conversion, is acting to maintain the homogeneity of the satellite DNA despite its abundance and distribution on all of the chromosomes. Images PMID:2762148

Production of biogenic amines in "Salamini italiani alla cacciatora PDO".

Science.gov (United States)

Coı X0308 Sson, Jean Daniel; Cerutti, Caterina; Travaglia, Fabiano; Arlorio, Marco

2004-06-01

Various fermented and seasoned foods such as cheese, sauerkraut, wine, beer and meat products may contain biogenic amines. The aim of this paper was to describe the presence of some biogenic amines (histamine, tyramine, tryptamine, 2-phenylethylamine) in "Salamini italiani alla cacciatora PDO", a typical fermented-ripened dry sausage widely consumed in Italy. Total level of biogenic amines in commercial samples ranged from 71 to 586 mg kg(-1). The amine recovered in higher concentrations was tyramine (372 mg kg(-1)) followed by histamine (165 mg kg(-1)). The second aim of this work was the quality control of the production in order to determine the parameters influencing the presence of biogenic amines in ripened salami. Sausages sampled for analysis during production, manipulation and ripening showed the presence of tyramine (64.4 mg kg(-1)) only after 15 days of fermentation. All investigated biogenic amines were detected in "Salamini" after 21 days of fermentation. We suggest the control of biogenic as important tool to establish the better condition of preservation of "Salamini italiani alla cacciatore PDO" during their shelf-life.

Plantago lagopus B Chromosome Is Enriched in 5S rDNA-Derived Satellite DNA

Czech Academy of Sciences Publication Activity Database

Kumke, K.; Macas, Jiří; Fuchs, J.; Altschmied, L.; Kour, J.; Dhar, M.K.; Houben, A.

2016-01-01

Roč. 148, č. 1 (2016), s. 68-73 ISSN 1424-8581 R&D Projects: GA ČR GBP501/12/G090 Institutional support: RVO:60077344 Keywords : Polymorhpic A chromosome segment * Satellite repeat * Supernumerary chromosome * 5S rDNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.354, year: 2016

Investigating unexpected magnetism of mesoporous silica-supported Pd and PdO nanoparticles

KAUST Repository

Song, Hyon Min; Zink, Jeffrey I.; Khashab, Niveen M.

2015-01-01

supported Pd and PdO NPs. The heating rate and the annealing conditions determine the particle size and the phase of the NPs, with a fast heating rate of 30 °C/min producing the largest supported Pd NPs. Unusual magnetic behaviors are observed. (1) Contrary

S1 satellite DNA repetitive units display identical structure and overall variability in all Anatolian brown frog taxa.

Science.gov (United States)

Picariello, Orfeo; Feliciello, Isidoro; Chinali, Gianni

2016-02-01

S1 satellite DNA from Palearctic brown frogs has a species-specific structure in all European species. We characterized S1 satellite DNA from the Anatolian brown frogs Rana macrocnemis, R. camerani, and R. holtzi in order to define their taxonomic rank and the structure of this satellite in this frog lineage. Southern blots of genomic DNA digested with KpnI, EcoRV, NdeI, NheI, or StuI produced the same pattern of satellite DNA bands. Moreover, quantitative dot blots showed that this satellite DNA accounts for 0.1 % of the genome in all taxa. Analysis of the overall genomic variability of the S1a repeat sequence in specimens from various populations demonstrated that this repetitive unit also has the same size (476 bp), the same most common sequence (MCS) and the same overall variability in all three taxa, and also in R. macrocnemis tavasensis. The S1a repetitive unit presents three deletions of 9, 8 and 1 bp compared to the 494-bp S1a repeat from European frogs. The S1a MCS has three variable positions (sequence WWTK in positions 183-186), due to the presence of two repeat subpopulations with motifs AATG and WWTT in all taxa. Unlike previously analyzed mitochondrial and nuclear sequences that show considerable variations among these taxa, no difference could be detected in the structure and variability of the S1 satellite repetitive units. This suggests that these taxa should belong to a single species. Our results indicate that this satellite DNA variety probably formed when the Anatolian lineage radiated from common ancestor about 4 mya, and since then has maintained its structure in all four taxa examined.

Characterization of non-coding DNA satellites associated with sweepoviruses (genus Begomovirus, Geminiviridae - definition of a distinct class of begomovirus-associated satellites

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Gloria eLozano

2016-02-01

Full Text Available Begomoviruses (family Geminiviridae are whitefly-transmitted, plant-infecting single-stranded DNA viruses that cause crop losses throughout the warmer parts of the World. Sweepoviruses are a phylogenetically distinct group of begomoviruses that infect plants of the family Convolvulaceae, including sweet potato (Ipomoea batatas. Two classes of subviral molecules are often associated with begomoviruses, particularly in the Old World; the betasatellites and the alphasatellites. An analysis of sweet potato and Ipomoea indica samples from Spain and Merremia dissecta samples from Venezuela identified small non-coding subviral molecules in association with several distinct sweepoviruses. The sequences of 18 clones were obtained and found to be structurally similar to tomato leaf curl virus–satellite (ToLCV-sat, the first DNA satellite identified in association with a begomovirus, with a region with significant sequence identity to the conserved region of betasatellites, an A-rich sequence, a predicted stem-loop structure containing the nonanucleotide TAATATTAC, and a second predicted stem-loop. These sweepovirus-associated satellites join an increasing number of ToLCV-sat-like non-coding satellites identified recently. Although sharing some features with betasatellites, evidence is provided to suggest that the ToLCV-sat-like satellites are distinct from betasatellites and should be considered a separate class of satellites, for which the collective name deltasatellites is proposed.

Greek PDO saffron authentication studies using species specific molecular markers.

Science.gov (United States)

Bosmali, I; Ordoudi, S A; Tsimidou, M Z; Madesis, P

2017-10-01

Saffron, the spice produced from the red stigmas of the flower of Crocus sativus L. is a frequent target of fraud and mislabeling practices that cannot be fully traced using the ISO 3632 trade standard specifications and test methods. A molecular approach is proposed herein as a promising branding strategy for the authentication of highly esteemed saffron brands such as the Greek Protected Designation of Origin (PDO) "Krokos Kozanis". Specific ISSR (inter-simple sequence repeat) markers were used to assess for the first time, the within species variability of several populations of C. sativus L. from the cultivation area of "Krokos Kozanis" as well as the potential differences with the band pattern produced by other Crocus species. Then, species-specific markers were developed taking advantage of an advanced molecular technique such as the HRM analysis coupled with universal DNA barcoding regions (trnL) (Bar-HRM) and applied to saffron admixtures with some of the most common plant adulterants (Calendula officinalis, Carthamus tinctorius, Gardenia jasminoides, Zea mays and Curcuma longa). The sensitivity of the procedure was tested for turmeric as a case study whereas HPLC-fluorescence determination of secondary metabolites was also employed for comparison. The overall results indicated that the Bar-HRM approach is quite effective in terms of specificity and sensitivity. Its effectiveness regarding the detection of turmeric was comparable to that of a conventional HPLC method (0.5% vs 1.0%, w/w). Yet, the proposed DNA-based method is much faster, cost-effective and can be used even by non-geneticists, in any laboratory having access to an HRM-capable real-time PCR instrumentation. It can be, thus, regarded as a strong analytical tool in saffron authentication studies. Copyright © 2017 Elsevier Ltd. All rights reserved.

Satellite DNA-based artificial chromosomes for use in gene therapy.

Science.gov (United States)

Hadlaczky, G

2001-04-01

Satellite DNA-based artificial chromosomes (SATACs) can be made by induced de novo chromosome formation in cells of different mammalian species. These artificially generated accessory chromosomes are composed of predictable DNA sequences and they contain defined genetic information. Prototype human SATACs have been successfully constructed in different cell types from 'neutral' endogenous DNA sequences from the short arm of the human chromosome 15. SATACs have already passed a number of hurdles crucial to their further development as gene therapy vectors, including: large-scale purification; transfer of purified artificial chromosomes into different cells and embryos; generation of transgenic animals and germline transmission with purified SATACs; and the tissue-specific expression of a therapeutic gene from an artificial chromosome in the milk of transgenic animals.

Multi-scale impacts of the Pacific SST and PDO on the summer precipitation of North-Central China from 1870 to 2002

Science.gov (United States)

Bi, Shuoben; Qu, Ying; Bi, Shengjie; Wu, Weiting; Jiang, Tingting

2018-05-01

Climate variability has become a hot topic worldwide in recent years. Although large numbers of climate data series have been reconstructed based on hundreds to thousands, or even tens of thousands, of years, our understanding of this problem is still controversial. We use the precipitation index (PI) series to study the periodicity of the precipitation in northern China from 1870 to 2002 and explore the climate variability on a large timescale. The analysis shows that the precipitation has periods of 2.27-3.03, 7.14, 10.00, 11.11, 12.50, 14.29, 16.67, 20.00, and 25.00 a. Based on complete ensemble empirical mode decomposition (CEEMD), the Pacific sea surface temperature (SST) and Pacific interdecadal oscillation (PDO) are decomposed into different frequencies. The results show that the SST and PDO have interannual to interdecadal periodicity. To determine the impact of the Pacific SST and PDO on the PI, we make use of the cross wavelet power spectrum and wavelet coherency spectrum to analyze their period relation and reveal their periodic change characteristics; it is found that different bands of the Pacific SST, PDO, and PI have high power.

Molecular Cytogenetic Mapping of Satellite DNA Sequences in Aegilops geniculata and Wheat

Czech Academy of Sciences Publication Activity Database

Koo, D.H.; Tiwari, V.K.; Hřibová, Eva; Doležel, Jaroslav; Friebe, B.; Gill, B.S.

2016-01-01

Roč. 148, č. 4 (2016), s. 314-321 ISSN 1424-8581 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : in-situ hybridization * chromosome addition lines * resistance genes lr57 * repetitive dna * triticum-ovatum * powdery mildew * plant genome * bread wheat * leaf rust * identification * Aegilops geniculata * Chromosome identification * Fluorescence in situ hybridization * Satellite DNA * Wheat Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.354, year: 2016

The impact of combined ENSO and PDO on the PNA climate: a 1,000-year climate modeling study

Energy Technology Data Exchange (ETDEWEB)

Yu, B. [Environment Canada, Climate Data and Analysis Section, Climate Research Division, Toronto, ON (Canada); Zwiers, F.W. [Environment Canada, Canadian Centre for Climate Modelling and Analysis, Climate Research Division, Victoria (Canada)

2007-12-15

This study analyzes the atmospheric response to the combined Pacific interannual ENSO and decadal-interdecadal PDO variability, with a focus on the Pacific-North American (PNA) sector, using a 1,000-year long integration of the Canadian Center for Climate Modelling and Analysis (CCCma) coupled climate model. Both the tropospheric circulation and the North American temperature suggest an enhanced PNA-like climate response and impacts on North America when ENSO and PDO variability are in phase. The anomalies of the centers of action for the PNA-like pattern are significantly different from zero and the anomaly pattern is field significant. In association with the stationary wave anomalies, large stationary wave activity fluxes appear in the mid-high latitudes originating from the North Pacific and flowing downstream toward North America. There are significant Rossby wave source anomalies in the extratropical North Pacific and in the subtropical North Pacific. In addition, the axis of the Pacific storm track shifts southward with the positive PNA. Atmospheric heating anomalies associated with ENSO variability are confined primarily to the tropics. There is an anomalous heating center over the northeast Pacific, together with anomalies with the same polarity in the tropical Pacific, for the PDO variability. The in-phase combination of ENSO and PDO would in turn provide anomalous atmospheric energy transports towards North America from both the Tropical Pacific and the North Pacific, which tends to favor the occurrence of stationary wave anomalies and would lead to a PNA-like wave anomaly structure. The modeling results also confirm our analysis based on the observational record in the twentieth century. (orig.)

Response of bacterial pdo1, nah, and C12O genes to aged soil PAH pollution in a coke factory area.

Science.gov (United States)

Han, Xue-Mei; Liu, Yu-Rong; Zheng, Yuan-Ming; Zhang, Xiao-Xia; He, Ji-Zheng

2014-01-01

Soil pollution caused by polycyclic aromatic hydrocarbons (PAHs) is threatening human health and environmental safety. Investigating the relative prevalence of different PAH-degrading genes in PAH-polluted soils and searching for potential bioindicators reflecting the impact of PAH pollution on microbial communities are useful for microbial monitoring, risk evaluation, and potential bioremediation of soils polluted by PAHs. In this study, three functional genes, pdo1, nah, and C12O, which might be involved in the degradation of PAHs from a coke factory, were investigated by real-time quantitative PCR (qPCR) and clone library approaches. The results showed that the pdo1 and C12O genes were more abundant than the nah gene in the soils. There was a significantly positive relationship between the nah or pdo1 gene abundances and PAH content, while there was no correlation between C12O gene abundance and PAH content. Analyses of clone libraries showed that all the pdo1 sequences were grouped into Mycobacterium, while all the nah sequences were classified into three groups: Pseudomonas, Comamonas, and Polaromonas. These results indicated that the abundances of nah and pdo1 genes were positively influenced by levels of PAHs in soil and could be potential microbial indicators reflecting the impact of soil PAH pollution and that Mycobacteria were one of the most prevalent PAHs degraders in these PAH-polluted soils. Principal component analysis (PCA) and correlation analyses between microbial parameters and environmental factors revealed that total carbon (TC), total nitrogen (TN), and dissolved organic carbon (DOC) had positive effects on the abundances of all PAH-degrading genes. It suggests that increasing TC, TN, and DOC inputs could be a useful way to remediate PAH-polluted soils.

Sea level reconstruction from satellite altimetry and tide gauge data

DEFF Research Database (Denmark)

Svendsen, Peter Limkilde; Andersen, Ole Baltazar; Nielsen, Allan Aasbjerg

2012-01-01

Ocean satellite altimetry has provided global sets of sea level data for the last two decades, allowing determination of spatial patterns in global sea level. For reconstructions going back further than this period, tide gauge data can be used as a proxy. We examine different methods of combining...... for better sensitivity analysis with respect to spatial distribution, and tide gauge data are available around the Arctic Ocean, which may be important for a later high-latitude reconstruction....... satellite altimetry and tide gauge data using optimal weighting of tide gauge data, linear regression and EOFs, including automatic quality checks of the tide gauge time series. We attempt to augment the model using various proxies such as climate indices like the NAO and PDO, and investigate alternative...

Characterisation of PDO olive oil Chianti Classico by non-selective (UV–visible, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques

International Nuclear Information System (INIS)

Casale, M.; Oliveri, P.; Casolino, C.; Sinelli, N.; Zunin, P.; Armanino, C.; Forina, M.; Lanteri, S.

2012-01-01

Highlights: ► Characterisation of the Italian PDO extra virgin olive oil Chianti Classico. ► Comparison between non-selective (UV–vis, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques. ► Synergy among spectroscopic techniques, by the fusion of the respective spectra. ► Prediction of the content of oleic and linoleic acids in the olive oils. - Abstract: An authentication study of the Italian PDO (protected designation of origin) extra virgin olive oil Chianti Classico was performed; UV–visible (UV–vis), Near-Infrared (NIR) and Mid-Infrared (MIR) spectroscopies were applied to a set of samples representative of the whole Chianti Classico production area. The non-selective signals (fingerprints) provided by the three spectroscopic techniques were utilised both individually and jointly, after fusion of the respective profile vectors, in order to build a model for the Chianti Classico PDO olive oil. Moreover, these results were compared with those obtained by the gas chromatographic determination of the fatty acids composition. In order to characterise the olive oils produced in the Chianti Classico PDO area, UNEQ (unequal class models) and SIMCA (soft independent modelling of class analogy) were employed both on the MIR, NIR and UV–vis spectra, individually and jointly, and on the fatty acid composition. Finally, PLS (partial least square) regression was applied on the UV–vis, NIR and MIR spectra, in order to predict the content of oleic and linoleic acids in the extra virgin olive oils. UNEQ, SIMCA and PLS were performed after selection of the relevant predictors, in order to increase the efficiency of both classification and regression models. The non-selective information obtained from UV–vis, NIR and MIR spectroscopy allowed to build reliable models for checking the authenticity of the Italian PDO extra virgin olive oil Chianti Classico.

Characterisation of PDO olive oil Chianti Classico by non-selective (UV-visible, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques

Energy Technology Data Exchange (ETDEWEB)

Casale, M., E-mail: monica@dictfa.unige.it [Universita degli Studi di Genova, Department of Chemistry and Food and Pharmaceutical Technologies, Via Brigata Salerno 13, I-16147, Genoa (Italy); Oliveri, P.; Casolino, C. [Universita degli Studi di Genova, Department of Chemistry and Food and Pharmaceutical Technologies, Via Brigata Salerno 13, I-16147, Genoa (Italy); Sinelli, N. [Universita degli Studi di Milano, Department of Food Science and Technology, Via Celoria, 2 - I-20133 Milan (Italy); Zunin, P.; Armanino, C.; Forina, M.; Lanteri, S. [Universita degli Studi di Genova, Department of Chemistry and Food and Pharmaceutical Technologies, Via Brigata Salerno 13, I-16147, Genoa (Italy)

2012-01-27

Highlights: Black-Right-Pointing-Pointer Characterisation of the Italian PDO extra virgin olive oil Chianti Classico. Black-Right-Pointing-Pointer Comparison between non-selective (UV-vis, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques. Black-Right-Pointing-Pointer Synergy among spectroscopic techniques, by the fusion of the respective spectra. Black-Right-Pointing-Pointer Prediction of the content of oleic and linoleic acids in the olive oils. - Abstract: An authentication study of the Italian PDO (protected designation of origin) extra virgin olive oil Chianti Classico was performed; UV-visible (UV-vis), Near-Infrared (NIR) and Mid-Infrared (MIR) spectroscopies were applied to a set of samples representative of the whole Chianti Classico production area. The non-selective signals (fingerprints) provided by the three spectroscopic techniques were utilised both individually and jointly, after fusion of the respective profile vectors, in order to build a model for the Chianti Classico PDO olive oil. Moreover, these results were compared with those obtained by the gas chromatographic determination of the fatty acids composition. In order to characterise the olive oils produced in the Chianti Classico PDO area, UNEQ (unequal class models) and SIMCA (soft independent modelling of class analogy) were employed both on the MIR, NIR and UV-vis spectra, individually and jointly, and on the fatty acid composition. Finally, PLS (partial least square) regression was applied on the UV-vis, NIR and MIR spectra, in order to predict the content of oleic and linoleic acids in the extra virgin olive oils. UNEQ, SIMCA and PLS were performed after selection of the relevant predictors, in order to increase the efficiency of both classification and regression models. The non-selective information obtained from UV-vis, NIR and MIR spectroscopy allowed to build reliable models for checking the authenticity of the Italian PDO extra virgin olive oil

Characterisation of PDO olive oil Chianti Classico by non-selective (UV-visible, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques.

Science.gov (United States)

Casale, M; Oliveri, P; Casolino, C; Sinelli, N; Zunin, P; Armanino, C; Forina, M; Lanteri, S

2012-01-27

An authentication study of the Italian PDO (protected designation of origin) extra virgin olive oil Chianti Classico was performed; UV-visible (UV-vis), Near-Infrared (NIR) and Mid-Infrared (MIR) spectroscopies were applied to a set of samples representative of the whole Chianti Classico production area. The non-selective signals (fingerprints) provided by the three spectroscopic techniques were utilised both individually and jointly, after fusion of the respective profile vectors, in order to build a model for the Chianti Classico PDO olive oil. Moreover, these results were compared with those obtained by the gas chromatographic determination of the fatty acids composition. In order to characterise the olive oils produced in the Chianti Classico PDO area, UNEQ (unequal class models) and SIMCA (soft independent modelling of class analogy) were employed both on the MIR, NIR and UV-vis spectra, individually and jointly, and on the fatty acid composition. Finally, PLS (partial least square) regression was applied on the UV-vis, NIR and MIR spectra, in order to predict the content of oleic and linoleic acids in the extra virgin olive oils. UNEQ, SIMCA and PLS were performed after selection of the relevant predictors, in order to increase the efficiency of both classification and regression models. The non-selective information obtained from UV-vis, NIR and MIR spectroscopy allowed to build reliable models for checking the authenticity of the Italian PDO extra virgin olive oil Chianti Classico. Copyright © 2011 Elsevier B.V. All rights reserved.

Methodological development for 87Sr/86Sr measurement in olive oil and preliminary discussion of its use for geographical traceability of PDO Nîmes (France).

Science.gov (United States)

Medini, Salim; Janin, Myriam; Verdoux, Patrick; Techer, Isabelle

2015-03-15

The lack of a geographical identification protocol for olive oils can lead to fraud and health risks. As some works call for Sr isotopes for the geographical identification of agri-food products, this study focus on the feasibility of extracting Sr from olive oils for isotopic measurements by TIMS. In fact, existing protocols for purification of Sr are unsuitable for lipid matrix. The defined protocol is applied to samples of PDO Nîmes olive oil. The accuracy of the extraction procedure is tested against isotopic standards. The values obtained are in conformity with NIST certified values. This consistency demonstrates that no modification of (87)Sr/(86)Sr ratio is brought about by this protocol. Consequently, the method is preliminary used on PDO Nîmes and Moroccan oils to evaluate the feasibility of a discriminant Sr signature on the two geographical products. This study provides promising results for the geographical discrimination and identification of PDO olive oils. Copyright © 2014 Elsevier Ltd. All rights reserved.

A General Strategy for the Preparation of Carbon Nanotubes and Graphene Oxide Decorated with PdO Nanoparticles in Water

Directory of Open Access Journals (Sweden)

Hongkun He

2010-07-01

Full Text Available The preparation of carbon nanotube (CNT/PdO nanoparticles and graphene oxide (GO/PdO nanoparticle hybrids via a general aqueous solution strategy is reported. The PdO nanoparticles are generated in situ on the CNTs and GO by a one-step “green” synthetic approach in aqueous Pd(NO32 solution under ambient conditions without adding any additional chemicals. The production of PdO is confirmed by energy dispersive X-ray spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy, and thermal gravimetric analysis. The morphologies of the resulting CNT/PdO and GO/PdO nanohybrids are characterized by transmission and/or scanning transmission electron microscopy. PdO nanoparticles with an average size of 2–3 nm in diameter are decorated evenly along the surfaces of CNTs and GO. This synthesis strategy is demonstrated to be compatible for 1 CNTs with different modifications, including pristine, oxidized, and polymer-functionalized CNTs; 2 different types of CNTs, including single-walled carbon nanotubes (SWCNTs, double-walled carbon nanotubes (DWCNTs, and multiwalled carbon nanotubes (MWCNTs; and 3 different shapes of carbon materials, including tubular CNTs and planar GO. The as-prepared CNT/PdO and GO/PdO nanohybrids can be transformed into CNT/Pd and GO/Pd nanohybrids by reduction with NaBH4, and can then be used as a heterogeneous catalyst in the catalytic reduction of 4-nitrophenol.

Different AT-rich satellite DNAs in Cucurbita pepo and Cucurbita maxima.

Science.gov (United States)

Ganal, M; Hemleben, V

1986-11-01

The AT-rich highly repeated satellite DNA of Cucurbita pepo (zucchini) and Cucurbita maxima (pumpkin) were cloned and their DNA structure was investigated. DNA sequencing revealed that the repeat length of satellite DNA in Cucurbita pepo is 349-352 base pairs. The percentage of AT-base pairs is about 61%. This satellite is highly conserved in restriction enzyme pattern and DNA sequence; sequence heterogeneity is about 10%. In contrast, the satellite DNA of Cucurbita maxima has a repeat length of 168-169 base pairs. This satellite is also rich in AT-base pairs (64%), existing in at least three different variants as revealed by restriction enzyme analysis and DNA sequencing. The sequence heterogeneity between these variants is about 15%. The two satellite DNAs showed no cross-hybridization to each other and sequence homology is only limited. Nevertheless, we found in the C. pepo genome a high amount of sequences resembling the satellite of C. maxima. In contrast, the satellite repeat of C. pepo is found in the C. maxima DNA only in a few copies. These observations were discussed with respect to satellite DNA evolution and compared to the data received from monocotyledonous species.

Association of pKi-67 with satellite DNA of the human genome in early G1 cells.

Science.gov (United States)

Bridger, J M; Kill, I R; Lichter, P

1998-01-01

pKi-67 is a nucleolar antigen that provides a specific marker for proliferating cells. It has been shown previously that pKi-67's distribution varies in a cell cycle-dependent manner: it coats all chromosomes during mitosis, accumulates in nuclear foci during G1 phase (type I distribution) and localizes within nucleoli in late G1 S and G2 phase (type II distribution). Although no function has as yet been ascribed to pKi-67, it has been found associated with centromeres in G1. In the present study the distribution pattern of pKi-67 during G1 in human dermal fibroblasts (HDFs) was analysed in more detail. Synchronization experiments show that in very early G1 cells pKi-67 coincides with virtually all satellite regions analysed, i.e. with centromeric (alpha-satellite), telomeric (minisatellite) and heterochromatic blocks (satellite III) on chromosomes 1 and Y (type Ia distribution). In contrast, later in the G1 phase, a smaller fraction of satellite DNA regions are found collocalized with pKi-67 foci (type Ib distribution). When all pKi-67 becomes localized within nucleoli, even fewer satellite regions remain associated with the pKi-67 staining. However, all centromeric and short arm regions of the acrocentric chromosomes, which are in very close proximity to or even contain the rRNA genes, are collocalized with anti-pKi-67 staining throughout the remaining interphase of the cell cycle. Thus, our data demonstrate that during post-mitotic reformation and nucleogenesis there is a progressive decline in the fraction of specific satellite regions of DNA that remain associated with pKi-67. This may be relevant to nucleolar reformation following mitosis.

Characterization of Satellite DNA Sequences from the Commercially Important Marine Rotifers Brachionus rotundiformis and Brachionus plicatilis.

Science.gov (United States)

Boehm; Gibson; Lubzens

2000-01-01

This study was initiated to search for species-specific and strain-specific satellite DNA sequences for which oligonucleotide primers could be designed to differentiate between various commercially important strains of the marine monogonont rotifers Brachionus rotundiformis and Brachionus plicatilis. Two unrelated, highly reiterated satellite sequences were cloned and characterized. The eight sequenced monomers from B. rotundiformis and six from B. plicatilis had low intrarepeat variability and were similar in their overall lengths, A + T compositions, and high degrees of repeated motif substructure. However, hybridizations to 19 representative strains, sequence characterizations, and GenBank searches indicated that these two satellites are morphotype-specific and population-specific, respectively, and share little homology to each other or to other characterized sequences in the database. Primer pairs designed for the B. rotundiformis satellite confirmed hybridization specificities on polymerase chain reaction and could serve as a useful molecular diagnostic tool to identify strains belonging to the SS morphotype, which are gaining widespread usage as first feeds for marine fish in commercial production.

Evaluation of DNA bending models in their capacity to predict electrophoretic migration anomalies of satellite DNA sequences.

Science.gov (United States)

Matyášek, Roman; Fulneček, Jaroslav; Kovařík, Aleš

2013-09-01

DNA containing a sequence that generates a local curvature exhibits a pronounced retardation in electrophoretic mobility. Various theoretical models have been proposed to explain relationship between DNA structural features and migration anomaly. Here, we studied the capacity of 15 static wedge-bending models to predict electrophoretic behavior of 69 satellite monomers derived from four divergent families. All monomers exhibited retarded mobility in PAGE corresponding to retardation factors ranging 1.02-1.54. The curvature varied both within and across the groups and correlated with the number, position, and lengths of A-tracts. Two dinucleotide models provided strong correlation between gel mobility and curvature prediction; two trinucleotide models were satisfactory while remaining dinucleotide models provided intermediate results with reliable prediction for subsets of sequences only. In some cases, similarly shaped molecules exhibited relatively large differences in mobility and vice versa. Generally less accurate predictions were obtained in groups containing less homogeneous sequences possessing distinct structural features. In conclusion, relatively universal theoretical models were identified suitable for the analysis of natural sequences known to harbor relatively moderate curvature. These models could be potentially applied to genome wide studies. However, in silico predictions should be viewed in context of experimental measurement of intrinsic DNA curvature. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PDO as a Mechanism for Reterritorialisation and Agri-Food Governance: A Comparative Analysis of Cheese Products in the UK and Switzerland

Directory of Open Access Journals (Sweden)

Damian Maye

2016-10-01

Full Text Available The protection of geographical indications (European regulation 1151/2012 is arguably the most significant initiative, certainly within Europe, that promotes foods with territorial associations and reorganises agri-food chain governance through a strategy of reterritorialisation. Research on Protected Designation of Origins (PDOs and Protected Geographical Indications (PGIs suggests that they generate significant economic value at an EU-level, especially in certain countries. They can also help to deliver territorial rural development policy and develop new food markets. In this paper we examine the way the PDO scheme has been developed and applied in one commodity sector (cheese in two countries (Switzerland and the UK, where the uptake of PDOs is variable. We adopt a food chain approach and examine specific cheese product case studies (at micro and meso levels in both countries to better understand how the PDO scheme (as a territorialisation and respacing strategy is implemented. L’Etivaz and Le Gruyère are examined in Switzerland. Single Gloucester and West Country cheddar are examined in the UK. The PDO scheme is an important governance strategy and regulatory system, but despite strict guidelines regarding implementation and geographical infrastructure there are notable differences between the UK and Switzerland in terms of how the label is used to organise and respatialise food chains: it is framed as a strategy to protect the rural economy in Switzerland but is promoted more as a mechanism to communicate and reconnect with consumers in the UK.

Investigation of sporadic solar radiowave radiation and Earth ionosphere parameters on the ''Interkosmos-Kopernik 500'' satellite

International Nuclear Information System (INIS)

Aksenov, V.I.; Artem'eva, u.M.; Komrakov, G.P.; Skrebkova, L.A.

1982-01-01

Results of investigations into inhomogeneities of electron concentration obtained in 1973 by means of '' the Interkosmos-Kopernik-500'' satellite are presented. A high-frequency impedance probe installed at the satellite (3.1 and 15 MHz working frequencies) provided measurement of the electron concentration in ionosphere in the ranges from 10 3 to 1.8x10 6 cm -3 and of its inhomogeneities of sizes from 0.5 to 100 km having relative fluctuations ΔN/N >= 3x10 -4 . Probability P of appearance of the electron concentration inhomogeneities with relative fluctuations √ (ΔN/N) 2 >= 0.2% at altitudes of 200-1500 km changes within the limits from 2 to 60-70% for middle and equatorial latitudes. The highest probability of the inhomogeneity appearance in the summer hemisphere (northern hemispere) is observed above the maximum of F 2 stratum. Values P at 400-1500 km altitudes at night-time increase from equatorial latitudes to middle ones. At Ksub(p) 2 at altitudes H > 400 km in the northern (summer) hemisphere are mainly lower than in the southern (winter). During the periods of increased geomagnetic activity (Ksub(p) > 3) the probabilities of the inhomogeneity appearance for the latitudes (middle and equatorial) under investigation change insignificantly as a whole. Power character of the inhomogeneous structures spectra at the altitudes of the F region and partially outer ionosphere at middle, low and equatorial latitudes of the northern and southern hemispheres is their common characteristic feature

Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome.

Science.gov (United States)

Waye, J S; Willard, H F

1986-09-01

The centromeric regions of all human chromosomes are characterized by distinct subsets of a diverse tandemly repeated DNA family, alpha satellite. On human chromosome 17, the predominant form of alpha satellite is a 2.7-kilobase-pair higher-order repeat unit consisting of 16 alphoid monomers. We present the complete nucleotide sequence of the 16-monomer repeat, which is present in 500 to 1,000 copies per chromosome 17, as well as that of a less abundant 15-monomer repeat, also from chromosome 17. These repeat units were approximately 98% identical in sequence, differing by the exclusion of precisely 1 monomer from the 15-monomer repeat. Homologous unequal crossing-over is suggested as a probable mechanism by which the different repeat lengths on chromosome 17 were generated, and the putative site of such a recombination event is identified. The monomer organization of the chromosome 17 higher-order repeat unit is based, in part, on tandemly repeated pentamers. A similar pentameric suborganization has been previously demonstrated for alpha satellite of the human X chromosome. Despite the organizational similarities, substantial sequence divergence distinguishes these subsets. Hybridization experiments indicate that the chromosome 17 and X subsets are more similar to each other than to the subsets found on several other human chromosomes. We suggest that the chromosome 17 and X alpha satellite subsets may be related components of a larger alphoid subfamily which have evolved from a common ancestral repeat into the contemporary chromosome-specific subsets.

Protected Designation of Origin (PDO), Protected Geographical Indication (PGI) and Traditional Speciality Guaranteed (TSG): A bibiliometric analysis.

Science.gov (United States)

Dias, Claudia; Mendes, Luís

2018-01-01

Despite the importance of the literature on food quality labels in the European Union (PDO, PGI and TSG), our search did not find any review joining the various research topics on this subject. This study aims therefore to consolidate the state of academic research in this field, and so the methodological option was to elaborate a bibliometric analysis resorting to the term co-occurrence technique. Analysis was made of 501 articles on the ISI Web of Science database, covering publications up to 2016. The results of the bibliometric analysis allowed identification of four clusters: "Protected Geographical Indication", "Certification of Olive Oil and Cultivars", "Certification of Cheese and Milk" and "Certification and Chemical Composition". Unlike the other clusters, where the PDO label predominates, the "Protected Geographical Indication" cluster covers the study of PGI products, highlighting analysis of consumer behaviour in relation to this type of product. The focus of studies in the "Certification of Olive Oil and Cultivars" cluster and the "Certification of Cheese and Milk" cluster is the development of authentication methods for certified traditional products. In the "Certification and Chemical Composition" cluster, standing out is analysis of the profiles of fatty acids present in this type of product. Copyright © 2017 Elsevier Ltd. All rights reserved.

Revealing climate modes in steric sea levels: lessons learned from satellite geodesy, objective analyses and ocean reanalyses

Science.gov (United States)

Pfeffer, J.; Tregoning, P.; Purcell, A. P.

2017-12-01

Due to increased greenhouse gases emissions, the oceans are accumulating heat. In response to the ocean circulation and atmospheric forcing, the heat is irregularly redistributed within the oceans, causing sea level to rise at variable rates in space and time. These rates of steric expansion are extremely difficult to assess because of the sparsity of in-situ hydrographic observations available within the course of the 20th century. We compare here three methods to reconstruct the steric sea levels over the past 13, 25 and 58 years based on satellite geodesy, objective analyses and ocean reanalyses. The interannual to decadal variability of each dataset is explored with a model merging six climate indices representative of the natural variability of the ocean and climate system. Consistent regional patterns are identified for the Pacific Decadal Oscillation (PDO) and El Niño Southern Oscillation (ENSO) in all datasets at all timescales. Despite the short time coverage (13 years), the combination of satellite geodetic data (altimetry and GRACE) also reveals significant steric responses to the North Pacific Gyre Oscillation (NPGO), Indian Dipole (IOD) and Indian ocean basinwide (IOBM) mode. The richer information content in the ocean reanalyses allows us to recover the regional fingerprints of the PDO, ENSO, NPGO, IOD and IOBM, but also of the Atlantic Multidecadal Oscillation (AMO) acting over longer time scales (40 to 60 years). Therefore, ocean reanalyses, coupled with climate mode analyses, constitute innovative and promising tools to investigate the mechanisms triggering the variability of sea level rise over the past decades.

Ab initio calculations and experimental measurement of the deuterium quadrupole coupling constant in Na2PDO3

International Nuclear Information System (INIS)

Trudeau, J.D.; Schwartz, J.L.; Farrar, T.C.

1991-01-01

The deuterium quadrupole coupling constant, χ D , in the PDO 3 2- anion has been measured in solution by NMR spin-lattice (T 1 ) relaxation time measurements and it has been calculated via ab initio methods. The experimental value of 94.7 ± 0.5 kHz is in excellent agreement with the ab initio value of 95.0 kHz. The activation energy for the ion reorientation is 2.23 ± 0.01 kJ mol -1

Identification of the Geographic Origin of Parmigiano Reggiano (P.D.O.) Cheeses Deploying Non-Targeted Mass Spectrometry and Chemometrics.

Science.gov (United States)

Popping, Bert; De Dominicis, Emiliano; Dante, Mario; Nocetti, Marco

2017-02-16

Parmigiano Reggiano is an Italian product with a protected designation of origin (P.D.O.). It is an aged hard cheese made from raw milk. P.D.O. products are protected by European regulations. Approximately 3 million wheels are produced each year, and the product attracts a relevant premium price due to its quality and all around the world well known typicity. Due to the high demand that exceeds the production, several fraudulent products can be found on the market. The rate of fraud is estimated between 20% and 40%, the latter predominantly in the grated form. We have developed a non-target method based on Liquid Chomatography-High Resolution Mass Spectrometry (LC-HRMS) that allows the discrimination of Parmigiano Reggiano from non-authentic products with milk from different geographical origins or products, where other aspects of the production process do not comply with the rules laid down in the production specifications for Parmeggiano Reggiano. Based on a database created with authentic samples provided by the Consortium of Parmigiano Reggiano Cheese, a reliable classification model was built. The overall classification capabilities of this non-targeted method was verified on 32 grated cheese samples. The classification was 87.5% accurate.

Classification of red wine based on its protected designation of origin (PDO) using Laser-induced Breakdown Spectroscopy (LIBS).

Science.gov (United States)

Moncayo, S; Rosales, J D; Izquierdo-Hornillos, R; Anzano, J; Caceres, J O

2016-09-01

This work reports on a simple and fast classification procedure for the quality control of red wines with protected designation of origin (PDO) by means of Laser Induced Breakdown Spectroscopy (LIBS) technique combined with Neural Networks (NN) in order to increase the quality assurance and authenticity issues. A total of thirty-eight red wine samples from different PDO were analyzed to detect fake wines and to avoid unfair competition in the market. LIBS is well known for not requiring sample preparation, however, in order to increase its analytical performance a new sample preparation treatment by previous liquid-to-solid transformation of the wine using a dry collagen gel has been developed. The use of collagen pellets allowed achieving successful classification results, avoiding the limitations and difficulties of working with aqueous samples. The performance of the NN model was assessed by three validation procedures taking into account their sensitivity (internal validation), generalization ability and robustness (independent external validation). The results of the use of a spectroscopic technique coupled with a chemometric analysis (LIBS-NN) are discussed in terms of its potential use in the food industry, providing a methodology able to perform the quality control of alcoholic beverages. Copyright © 2016 Elsevier B.V. All rights reserved.

Marketing Strategies Based on Consumer Preferences of Karnavas Mullberry Molasses with Protected Designation of Origin (PDO

Directory of Open Access Journals (Sweden)

Yavuz Topcu

2017-07-01

Full Text Available The aim of the study is to determine the integrated marketing tactic and strategies based on the main factors affecting the preferences and purchase patterns related to Karnavas Mullberry Molasses with PDO of the consumers dwelling in Erzurum. The main material of the study was provided by the primary data obtained from a survey conducted on 401 households residing in Erzurum during 2015, and consuming Karnavas Mullberry Molasses. Principal Component Analysis (PCA and Two-step Cluster Analysis were used to determine the main factors impacting on their purchase decisions, and then to segment homogenous clusters according to their purchase frequencies by taking into consideration the data, respectively. The results of the study indicated that the heavy and medium users who consume Karnavas Mullberry Molasses pointed out the necessity of the augmented and actual product image positioning at retail level under local individual brands considering the attributes of the holistic quality with PDO label and those of the sensory quality obtained from traditional production methods to contribute to rural development under the direct marketing approaches, respectively. On the other hand, the light users also focused on the increase of market penetration effect via promotion mix and the product positioning under the local branded actual product image implementing traditional production method and hedonic quality approaches. It could be applied the positioning and promotion strategies according to the consumers’ utility expectation and the product images, therefore, by considering their requirements and desires in each segment.

Fast UPLC/PDA determination of squalene in Sicilian P.D.O. pistachio from Bronte: Optimization of oil extraction method and analytical characterization.

Science.gov (United States)

Salvo, Andrea; La Torre, Giovanna Loredana; Di Stefano, Vita; Capocchiano, Valentina; Mangano, Valentina; Saija, Emanuele; Pellizzeri, Vito; Casale, Katia Erminia; Dugo, Giacomo

2017-04-15

A fast reversed-phase UPLC method was developed for squalene determination in Sicilian pistachio samples that entry in the European register of the products with P.D.O. In the present study the SPE procedure was optimized for the squalene extraction prior to the UPLC/PDA analysis. The precision of the full analytical procedure was satisfactory and the mean recoveries were 92.8±0.3% and 96.6±0.1% for 25 and 50mgL -1 level of addition, respectively. Selected chromatographic conditions allowed a very fast squalene determination; in fact it was well separated in ∼0.54min with good resolution. Squalene was detected in all the pistachio samples analyzed and the levels ranged from 55.45-226.34mgkg -1 . Comparing our results with those of other studies it emerges that squalene contents in P.D.O. Sicilian pistachio samples, generally, were higher than those measured for other samples of different geographic origins. Copyright © 2016 Elsevier Ltd. All rights reserved.

Human circulating ribosomal DNA content significantly increases while circulating satellite III (1q12) content decreases under chronic occupational exposure to low-dose gamma- neutron and tritium beta-radiation

Energy Technology Data Exchange (ETDEWEB)

Korzeneva, Inna B., E-mail: inna.korzeneva@molgen.vniief.ru [Russian Federal Nuclear Center – All-Russian Research Institute of Experimental Physics (RFNC-VNIIEF) 607190 Sarov, 37 Mira ave., Nizhniy Novgorod Region (Russian Federation); Kostuyk, Svetlana V. [Research Centre for Medical Genetics, 115478 Moscow, 1 Moskvorechye str. (Russian Federation); Ershova, Elizaveta S. [Research Centre for Medical Genetics, 115478 Moscow, 1 Moskvorechye str. (Russian Federation); V. A. Negovsky Research Institute of General Reanimatology, Moscow, 107031 (Russian Federation); Skorodumova, Elena N.; Zhuravleva, Veronika F.; Pankratova, Galina V.; Volkova, Irina V.; Stepanova, Elena V. [Russian Federal Nuclear Center – All-Russian Research Institute of Experimental Physics (RFNC-VNIIEF) 607190 Sarov, 37 Mira ave., Nizhniy Novgorod Region (Russian Federation); Porokhovnik, Lev N. [Research Centre for Medical Genetics, 115478 Moscow, 1 Moskvorechye str. (Russian Federation); Veiko, Natalia N. [Research Centre for Medical Genetics, 115478 Moscow, 1 Moskvorechye str. (Russian Federation); V. A. Negovsky Research Institute of General Reanimatology, Moscow, 107031 (Russian Federation)

2016-09-15

Highlights: • A transcribed region of human ribosomal repeat is resistant to double-strand breaks in the environment of a raised endonuclease activity. • Hybridization-based techniques are preferable for the analysis of damaged and/or oxidized genomic fragments, rather than the qRT-PCR method. • A chronic exposure to the low-dose IR induces an elevation of the rDNA content in the human circulating cfDNA as compared to cellular DNA. • An exposure to IR entails a decrease of the level of the human circulating satellite III (1q12) as compared to cellular DNA (RsatIII index). • The RrDNA/RsatIII ratio is a potential marker of a chronic IR individual exposure. - Abstract: A single exposure to ionizing radiation (IR) results in an elevated cell-free DNA (cfDNA) content in the blood plasma. In this case, the cfDNA concentration can be a marker of the cell death in the organism. However, a chronic exposure to a low-dose IR enhances both the endonuclease activity and titer of antibodies to DNA in blood plasma, resulting in a decrease of the total concentration of circulating cfDNA in exposed people. In this case, the total cfDNA concentration should not be considered as a marker of the cell death in an exposed body. We assumed that a pool of the cfDNA circulating in the exposed people contains DNA fragments, which are resistant to a double-strand break formation in the environment of the elevated plasma endonuclease activity, and can be accumulated in the blood plasma. In order to test this hypothesis, we studied the content of GC-rich sequences (69%GC) of the transcribed region of human ribosomal repeat (rDNA), as well as the content of AT-rich repeat (63%AT) of satellite III (1q12) in the cfDNA samples obtained from 285 individuals. We have found that a chronic exposure to gamma-neutron radiation (N = 88) and tritium β-radiation (N = 88) evokes an increase of the rDNA content (RrDNA index) and a decrease of the satellite III content (RsatIII index) in the

Human circulating ribosomal DNA content significantly increases while circulating satellite III (1q12) content decreases under chronic occupational exposure to low-dose gamma- neutron and tritium beta-radiation

International Nuclear Information System (INIS)

Korzeneva, Inna B.; Kostuyk, Svetlana V.; Ershova, Elizaveta S.; Skorodumova, Elena N.; Zhuravleva, Veronika F.; Pankratova, Galina V.; Volkova, Irina V.; Stepanova, Elena V.; Porokhovnik, Lev N.; Veiko, Natalia N.

2016-01-01

Highlights: • A transcribed region of human ribosomal repeat is resistant to double-strand breaks in the environment of a raised endonuclease activity. • Hybridization-based techniques are preferable for the analysis of damaged and/or oxidized genomic fragments, rather than the qRT-PCR method. • A chronic exposure to the low-dose IR induces an elevation of the rDNA content in the human circulating cfDNA as compared to cellular DNA. • An exposure to IR entails a decrease of the level of the human circulating satellite III (1q12) as compared to cellular DNA (RsatIII index). • The RrDNA/RsatIII ratio is a potential marker of a chronic IR individual exposure. - Abstract: A single exposure to ionizing radiation (IR) results in an elevated cell-free DNA (cfDNA) content in the blood plasma. In this case, the cfDNA concentration can be a marker of the cell death in the organism. However, a chronic exposure to a low-dose IR enhances both the endonuclease activity and titer of antibodies to DNA in blood plasma, resulting in a decrease of the total concentration of circulating cfDNA in exposed people. In this case, the total cfDNA concentration should not be considered as a marker of the cell death in an exposed body. We assumed that a pool of the cfDNA circulating in the exposed people contains DNA fragments, which are resistant to a double-strand break formation in the environment of the elevated plasma endonuclease activity, and can be accumulated in the blood plasma. In order to test this hypothesis, we studied the content of GC-rich sequences (69%GC) of the transcribed region of human ribosomal repeat (rDNA), as well as the content of AT-rich repeat (63%AT) of satellite III (1q12) in the cfDNA samples obtained from 285 individuals. We have found that a chronic exposure to gamma-neutron radiation (N = 88) and tritium β-radiation (N = 88) evokes an increase of the rDNA content (RrDNA index) and a decrease of the satellite III content (RsatIII index) in the

Detection of minor and major satellite DNA in cytokinesis-blocked mouse splenocytes by a PRINS tandem labelling approach.

Science.gov (United States)

Russo, A; Tommasi, A M; Renzi, L

1996-11-01

A protocol for the simultaneous visualization of minor and major satellite DNA by primed in situ DNA synthesis (PRINS) was developed in cytokinesis-blocked murine splenocytes. After individuation of optimal experimental conditions, a micronucleus (MN) test was carried out by treating splenocytes in vitro with the clastogenic agent mitomycin C and the aneugenic compound Colcemid. It was found that PRINS gives highly reproducible results, also comparable with the literature on MN results obtained by fluorescent in situ hybridization (FISH). Therefore the PRINS methodology may be proposed as a fast alternative to FISH for the characterization of induced MN.

Teleconnections in Groundwater of U.S. Principal Aquifers to the Non-Stationarity of ENSO, NAO, PDO, and AMO

Science.gov (United States)

Gurdak, J. J.; Kuss, A. M.

2012-12-01

Groundwater will play an important role in society's adaptation to climate variability and change. Therefore, it is particularly important to detect and quantify teleconnections in groundwater with non-stationarity in climate variability on interannual to multidecadal timescales because of the tangible and near-term implications for water-resource management. Interannual to multidecadal climate variability partially controls precipitation distribution in space and time, drought frequency and severity, snowmelt runoff, streamflow, and other hydrologic processes that profoundly affects surface-water resources. However, the effects of interannual to multidecadal climate variability on recharge rates and mechanisms and other subsurface hydrologic processes that affect groundwater quantity and quality are largely unknown in most aquifers of the United States (U.S.) and other regions of the world. Here we use singular spectrum analysis (SSA), wavelet coherence analysis, and lag correlation to quantify the effects of the El Niño Southern Oscillation (ENSO) (2-7 year cycle), North Atlantic Oscillation (NAO) (3-6 year cycle), Pacific Decadal Oscillation (PDO) (10-25 year cycle), and Atlantic Multidecadal Oscillation (AMO) (50-70 year cycle) on precipitation, groundwater levels, simulated groundwater pumping, and climate varying recharge rates across the regionally extensive Central Valley (52,000 km2), Basin and Range (700,000 km2), High Plains (450,000 km2), and North Atlantic Coastal Plain (130,000 km2) Principal Aquifers (PAs) of the U.S. The results indicate that precipitation, recharge, and groundwater levels are partially affected by interannual to multidecadal climate variability and groundwater-level fluctuations are not solely a function of temporal patterns in pumping. ENSO and PDO have a greater control than NAO and AMO on variability in precipitation and groundwater levels across the U.S., particularly in the western and central PAs. At many locations, recharge

Obesity-induced sperm DNA methylation changes at satellite repeats are reprogrammed in rat offspring

Directory of Open Access Journals (Sweden)

Neil A Youngson

2016-01-01

Full Text Available There is now strong evidence that the paternal contribution to offspring phenotype at fertilisation is more than just DNA. However, the identity and mechanisms of this nongenetic inheritance are poorly understood. One of the more important questions in this research area is: do changes in sperm DNA methylation have phenotypic consequences for offspring? We have previously reported that offspring of obese male rats have altered glucose metabolism compared with controls and that this effect was inherited through nongenetic means. Here, we describe investigations into sperm DNA methylation in a new cohort using the same protocol. Male rats on a high-fat diet were 30% heavier than control-fed males at the time of mating (16-19 weeks old, n = 14/14. A small (0.25% increase in total 5-methyl-2Ͳ-deoxycytidine was detected in obese rat spermatozoa by liquid chromatography tandem mass spectrometry. Examination of the repetitive fraction of the genome with methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq and pyrosequencing revealed that retrotransposon DNA methylation states in spermatozoa were not affected by obesity, but methylation at satellite repeats throughout the genome was increased. However, examination of muscle, liver, and spermatozoa from male 27-week-old offspring from obese and control fathers (both groups from n = 8 fathers revealed that normal DNA methylation levels were restored during offspring development. Furthermore, no changes were found in three genomic imprints in obese rat spermatozoa. Our findings have implications for transgenerational epigenetic reprogramming. They suggest that postfertilization mechanisms exist for normalising some environmentally-induced DNA methylation changes in sperm cells.

PdO doping tunes band-gap energy levels as well as oxidative stress responses to a Co₃O₄ p-type semiconductor in cells and the lung.

Science.gov (United States)

Zhang, Haiyuan; Pokhrel, Suman; Ji, Zhaoxia; Meng, Huan; Wang, Xiang; Lin, Sijie; Chang, Chong Hyun; Li, Linjiang; Li, Ruibin; Sun, Bingbing; Wang, Meiying; Liao, Yu-Pei; Liu, Rong; Xia, Tian; Mädler, Lutz; Nel, André E

2014-04-30

We demonstrate through PdO doping that creation of heterojunctions on Co3O4 nanoparticles can quantitatively adjust band-gap and Fermi energy levels to study the impact of metal oxide nanoparticle semiconductor properties on cellular redox homeostasis and hazard potential. Flame spray pyrolysis (FSP) was used to synthesize a nanoparticle library in which the gradual increase in the PdO content (0-8.9%) allowed electron transfer from Co3O4 to PdO to align Fermi energy levels across the heterojunctions. This alignment was accompanied by free hole accumulation at the Co3O4 interface and production of hydroxyl radicals. Interestingly, there was no concomitant superoxide generation, which could reflect the hole dominance of a p-type semiconductor. Although the electron flux across the heterojunctions induced upward band bending, the E(c) levels of the doped particles showed energy overlap with the biological redox potential (BRP). This allows electron capture from the redox couples that maintain the BRP from -4.12 to -4.84 eV, causing disruption of cellular redox homeostasis and induction of oxidative stress. PdO/Co3O4 nanoparticles showed significant increases in cytotoxicity at 25, 50, 100, and 200 μg/mL, which was enhanced incrementally by PdO doping in BEAS-2B and RAW 264.7 cells. Oxidative stress presented as a tiered cellular response involving superoxide generation, glutathione depletion, cytokine production, and cytotoxicity in epithelial and macrophage cell lines. A progressive series of acute pro-inflammatory effects could also be seen in the lungs of animals exposed to incremental PdO-doped particles. All considered, generation of a combinatorial PdO/Co3O4 nanoparticle library with incremental heterojunction density allowed us to demonstrate the integrated role of E(v), E(c), and E(f) levels in the generation of oxidant injury and inflammation by the p-type semiconductor, Co3O4.

DETECTION AND CHARACTERISATION OF LISTERIA ISOLATES COLLECTED ALONG THE GORGONZOLA PDO PRODUCTION CHAIN

Directory of Open Access Journals (Sweden)

D. Nucera

2011-08-01

Full Text Available Considering that Listeria monocytogenes represents a concern in the production of Gorgonzola PDO cheese, this study was aimed to investigate the presence of Listeria along one Gorgonzola PDO production chain. L. monocytogenes subtype diversity was also investigated in order to identify possible reservoirs and dissemination routes. One dairy plant and its conferring farms (N=20 were selected and samples were collected in 4 visits in order to investigate seasonal variability. In each of them, producer tank milk (N=80, Gorgonzola producer tanks (N=12, Gorgonzola producer environmental samples (N=108 were collected, for a total of 200 samples. L. innocua was isolated from 3 milk samples (4%; L. monocytogenes alone was detected in 3 environmental samples (16% and L. innocua in 11 (58%. Both species were present in 5 samples. All Listeria strains isolated were typed through ERIC and REP PCRs. For each samples, if possible 5 colonies were selected for typing, for a total of 76 isolates. The analyses allowed the differentiation of L. monocytogenes and L. innocua in 9 and 7 PCR profiles (P, respectively. The former showed an overall similarity value of 75% and the latter of 81%. P1 (N=3 of L. monocytogenes was present in multiple sources (ripening surfaces and salting equipment and P5 (N=6 in different sampling rounds (January and June 2009. P1 (N=25 and P2 (N=15 of L. innocua were found in multiple sources (performing equipment, ripening surfaces, salting equipment, moving carts and in multiple sample rounds (P1: May 2008, January and June 2009; P2: June and October 2009. The presence of a few highly similar strains of L. monocytogenes in the production chain, as suggested by the profiles retrieved from different sources/sampling rounds, indicates the presence of persistent and niche-adapted strains. However, no profile was shared between raw milk and environmental samples, suggesting that the plant environment, not the incoming raw milk, is the source

Human circulating ribosomal DNA content significantly increases while circulating satellite III (1q12) content decreases under chronic occupational exposure to low-dose gamma- neutron and tritium beta-radiation.

Science.gov (United States)

Korzeneva, Inna B; Kostuyk, Svetlana V; Ershova, Elizaveta S; Skorodumova, Elena N; Zhuravleva, Veronika F; Pankratova, Galina V; Volkova, Irina V; Stepanova, Elena V; Porokhovnik, Lev N; Veiko, Natalia N

A single exposure to ionizing radiation (IR) results in an elevated cell-free DNA (cfDNA) content in the blood plasma. In this case, the cfDNA concentration can be a marker of the cell death in the organism. However, a chronic exposure to a low-dose IR enhances both the endonuclease activity and titer of antibodies to DNA in blood plasma, resulting in a decrease of the total concentration of circulating cfDNA in exposed people. In this case, the total cfDNA concentration should not be considered as a marker of the cell death in an exposed body. We assumed that a pool of the cfDNA circulating in the exposed people contains DNA fragments, which are resistant to a double-strand break formation in the environment of the elevated plasma endonuclease activity, and can be accumulated in the blood plasma. In order to test this hypothesis, we studied the content of GC-rich sequences (69%GC) of the transcribed region of human ribosomal repeat (rDNA), as well as the content of AT-rich repeat (63%AT) of satellite III (1q12) in the cfDNA samples obtained from 285 individuals. We have found that a chronic exposure to gamma-neutron radiation (N=88) and tritium β-radiation (N=88) evokes an increase of the rDNA content (RrDNA index) and a decrease of the satellite III content (RsatIII index) in the circulating cfDNA as compared with the cfDNA of non-exposed people (N=109). Such index that simultaneously displays both the increase of rDNA content and decrease of satellite III content in the cfDNA (RrDNA/RsatIII) can be recommended as a marker of chronic processes in the body that involve the elevated cell death rate and/or increased blood plasma endonuclease activity. Copyright © 2016 Elsevier B.V. All rights reserved.

The decadal-scale variation of the South Asian summer monsoon onset and its connection with the PDO

Science.gov (United States)

Watanabe, T.; Yamazaki, K.

2013-12-01

The summer Asian monsoon shows the abrupt increase of precipitation on the onset phase. It is an interesting and important problem when the summer monsoon onset occurs because natural resources, such as water and renewable energy agricultural product, are influenced by the variation of the summer Asian monsoon. Some researchers suggested the advance of the Asian summer monsoon onset in recent decades. We investigated the variation of the Asian monsoon onset using the long-term onset data over Kerala, a state in the southwest region of India, for 1948-2011. We discuss three main questions: 1) how is the variation of the monsoon onset date in the long-term period, 2) how the variation of the onset date is related to variations of atmospheric circulation and SST, and 3) what is the mechanism of such variation. Our main method is composite analysis using monthly-mean data. Though the onset date over Kerala shows the trend toward the early onset in recent three decades, such a trend is not observed in the whole period. It is noteworthy that the onset over Kerala shows the interannual variation on a multi-decadal scale. As regards the early onset years of Kerala, the summer monsoon onset is early over the following regions: the region from the southern Arabian Sea to southwestern India, the region from the southern Bay of Bengal to the Indochina Peninsula and the western North Pacific Ocean. On the other hand, the onset is late over southern China, Taiwan and the northern Philippine Sea. In early onset years of Kerala, the sea surface temperature over the northern Pacific Ocean is very similar to the negative PDO. The stationary wave train related with the negative PDO reaches into the Central Asia region, generates warm anomaly there and hence intensifies the land-sea thermal contrast there, which promotes the summer monsoon onset over South and Southeast Asia. Though the correlation between the onset over Kerala and the PDO is weak before 1976, it becomes high after

DNA hairpin structures in solution: 500-MHz two-dimensional 1H NMR studies on d(CGCCGCAGC) and d(CGCCGTAGC)

International Nuclear Information System (INIS)

Gupta, G.; Sarma, M.H.; Sarma, R.H.

1987-01-01

A hairpin structure contains two conformationally distinct domains: a double-helical stem with Watson-Crick base pairs and a single-stranded loop that connects the two arms of the stem. By extensive 1D and 2D 500-MHz 1 H NMR studies in H 2 O and D 2 O, it has been demonstrated that the DNA oligomers d(CGCCGCAGC) and d(CGCCGTAGC) form hairpin structures under conditions of low concentration, 0.5 mM in DNA strand, and low salt (20 mM NaCl, pH 7). From examination of the nuclear Overhauser effect (NOE) between base protons H8/H6 and sugar protons H1' and H2'/H2'', it was concluded that in D(CGCCGCAGC) and d(CGCCCTAGC) all the nine nucleotides display average (C2'-endo,anti) geometry. The NMR data in conjunction with molecular model building and solvent accessibility studies were used to derive a working model for the hairpins

PdO Doping Tunes Band-Gap Energy Levels as Well as Oxidative Stress Responses to a Co3O4p-Type Semiconductor in Cells and the Lung

Science.gov (United States)

2014-01-01

We demonstrate through PdO doping that creation of heterojunctions on Co3O4 nanoparticles can quantitatively adjust band-gap and Fermi energy levels to study the impact of metal oxide nanoparticle semiconductor properties on cellular redox homeostasis and hazard potential. Flame spray pyrolysis (FSP) was used to synthesize a nanoparticle library in which the gradual increase in the PdO content (0–8.9%) allowed electron transfer from Co3O4 to PdO to align Fermi energy levels across the heterojunctions. This alignment was accompanied by free hole accumulation at the Co3O4 interface and production of hydroxyl radicals. Interestingly, there was no concomitant superoxide generation, which could reflect the hole dominance of a p-type semiconductor. Although the electron flux across the heterojunctions induced upward band bending, the Ec levels of the doped particles showed energy overlap with the biological redox potential (BRP). This allows electron capture from the redox couples that maintain the BRP from −4.12 to −4.84 eV, causing disruption of cellular redox homeostasis and induction of oxidative stress. PdO/Co3O4 nanoparticles showed significant increases in cytotoxicity at 25, 50, 100, and 200 μg/mL, which was enhanced incrementally by PdO doping in BEAS-2B and RAW 264.7 cells. Oxidative stress presented as a tiered cellular response involving superoxide generation, glutathione depletion, cytokine production, and cytotoxicity in epithelial and macrophage cell lines. A progressive series of acute pro-inflammatory effects could also be seen in the lungs of animals exposed to incremental PdO-doped particles. All considered, generation of a combinatorial PdO/Co3O4 nanoparticle library with incremental heterojunction density allowed us to demonstrate the integrated role of Ev, Ec, and Ef levels in the generation of oxidant injury and inflammation by the p-type semiconductor, Co3O4. PMID:24673286

Similarity of satellite DNA properties in the order Rodentia

Energy Technology Data Exchange (ETDEWEB)

Mazrimas, J A; Hatch, F T

1977-09-01

We have characterized satellite DNAs from 9 species of kangaroo rat (Dipodomys) and have shown that the HS-..cap alpha.. and HS-..beta.. satellites, where present, are nearly identical in all species as to melting transition midpoint (Tm), and density in neutral CsCl, alkaline CsCl, and Cs/sub 2/SO/sub 4/-Ag/sup +/ gradients. However, the MS satellites exist in two internally similar classes. The satellite DNAs from three other rodents were characterized (densities listed are in neutral CsCl). The pocket gopher, Thomomys bottae, contains Th-..cap alpha.. (1.713 g/ml) and Th-..beta.. (1.703 g/ml). The guinea pig (Cavia porcellus) contains Ca-..cap alpha.., Ca-..beta.., and Ca-..gamma.. at densities of 1.706 g/ml, 1.704 g/ml, and 1.704 g/ml, respectively. The antelope ground squirrel (Ammospermophilus harrisi) contains Am-..cap alpha.., 1.708 g/ml, Am-..beta.., 1.717 g/ml, and Am-..gamma.., 1.707 g/ml. The physical and chemical properties of the alpha-satellites from the above four rodents representing four different families in two suborders of Rodentia were compared. They show nearly identical Tm, nucleoside composition of single strands, and single strand densities in alkaline CsCl. Similar comparisons on the second or third satellite DNAs from these rodents also indicate a close relationship to each other. Thus the high degree of similarity of satellite sequences found in such a diverse group of rodents suggests a cellular function that is subject to natural selection, and implies that these sequences have been conserved over a considerable span of evolutionary time since the divergence of these rodents about 50 million years ago.

Similarity of satellite DNA properties in the order Rodentia

Energy Technology Data Exchange (ETDEWEB)

Mazrimas, J A; Hatch, F T

1977-09-01

Satellite DNAs from 9 species of kangaroo rat (Dipodomys) have been characterized and have shown that the HS-..cap alpha.. and HS-..beta.. satellites, where present, are nearly identical in all species as to melting transition midpoint (Tm), and density in neutral CsCl, alkaline CsCl, and Cs/sub 2/SO/sub 4/-Ag/sup +/ gradients. However, the MS satellites exist in two internally similar classes. The satellite DNAs from three other rodents were characterized (densities listed are in neutral CsCl). The pocket gopher, Thomomys bottae, contains Th-..cap alpha.. (1.713 g/ml) and Th..beta.. (1.703 g/ml). The guinea pig (Cavia porcellus) contains Ca-..cap alpha.., Ca-..beta.. and Ca-..gamma.. at densities of 1.706 g/ml, 1.704 g/ml and 1.704 g/ml, respectively. The antelope ground squirrel (Ammospermophilus harrisi) contains Am-..cap alpha.., 1.708 g/ml, Am-..beta.., 1.717 g/ml, and Am-..gamma.., 1.707 g/ml. The physical and chemical properties of the alpha-satellites from the above four rodents representing four different families in two suborders of Rodentia were compared. They show nearly identical Tm, nucleoside composition of single strands, and single strand densities in alkaline CsCl. Similar comparisons on the second or third satellite DNAs from these rodents also indicate a close relationship to each other. Thus the high degree of similarity of satellite sequences found in such a diverse group of rodents suggests a cellular function that is subject to natural selection, and implies that these sequences have been conserved over a considerable span of evolutionary time since the divergence of these rodents about 50 million years ago.

The Trypanosoma cruzi satellite DNA OligoC-TesT and Trypanosoma cruzi kinetoplast DNA OligoC-TesT for diagnosis of Chagas disease: a multi-cohort comparative evaluation study.

Directory of Open Access Journals (Sweden)

Koen De Winne

Full Text Available BACKGROUND: The Trypanosoma cruzi satellite DNA (satDNA OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. METHODOLOGY/PRINCIPAL FINDINGS: We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%-99.8% for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%-99.1% for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%-74.2% for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%-84.4% for the kDNA OligoC-Test. CONCLUSIONS/SIGNIFICANCE: Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p = 0.0004 higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT.

The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study

Science.gov (United States)

De Winne, Koen; Büscher, Philippe; Luquetti, Alejandro O.; Tavares, Suelene B. N.; Oliveira, Rodrigo A.; Solari, Aldo; Zulantay, Ines; Apt, Werner; Diosque, Patricio; Monje Rumi, Mercedes; Gironès, Nuria; Fresno, Manuel; Lopez-Velez, Rogelio; Perez-Molina, José A.; Monge-Maillo, Begoña; Garcia, Lineth; Deborggraeve, Stijn

2014-01-01

Background The Trypanosoma cruzi satellite DNA (satDNA) OligoC-TesT is a standardised PCR format for diagnosis of Chagas disease. The sensitivity of the test is lower for discrete typing unit (DTU) TcI than for TcII-VI and the test has not been evaluated in chronic Chagas disease patients. Methodology/Principal Findings We developed a new prototype of the OligoC-TesT based on kinetoplast DNA (kDNA) detection. We evaluated the satDNA and kDNA OligoC-TesTs in a multi-cohort study with 187 chronic Chagas patients and 88 healthy endemic controls recruited in Argentina, Chile and Spain and 26 diseased non-endemic controls from D.R. Congo and Sudan. All specimens were tested in duplicate. The overall specificity in the controls was 99.1% (95% CI 95.2%–99.8%) for the satDNA OligoC-TesT and 97.4% (95% CI 92.6%–99.1%) for the kDNA OligoC-TesT. The overall sensitivity in the patients was 67.9% (95% CI 60.9%–74.2%) for the satDNA OligoC-TesT and 79.1% (95% CI 72.8%–84.4%) for the kDNA OligoC-Test. Conclusions/Significance Specificities of the two T. cruzi OligoC-TesT prototypes are high on non-endemic and endemic controls. Sensitivities are moderate but significantly (p = 0.0004) higher for the kDNA OligoC-TesT compared to the satDNA OligoC-TesT. PMID:24392177

A tale of ENSO, PDO, and increasing aridity impacts on drought-deciduous shrubs in the Death Valley region.

Science.gov (United States)

Ehleringer, James R; Sandquist, Darren R

2018-06-28

Germination, establishment, phenology, and death among three drought-deciduous shrubs were influenced by ENSO/PDO and precipitation, based on 37 years of annual surveys. Encelia farinosa forms near monospecific stands on slopes, whereas E. frutescens and Ambrosia salsola dominate wash habitats. All shrubs exhibited phenological coherence. While germination, establishment, and mortality patterns were similar among wash species, these dynamics contrasted with E. farinosa on slopes. Germination was associated with El Niño years. Slope plant establishment was dependent on precipitation in the subsequent year, but not evidently so in wash species. Major mortality events were episodic, with Encelia mortality just as likely to occur in years with below or above average precipitation. In both Encelia species, mortality was associated with transitions to a cold PDO phase. In E. frutescens this response was more rapid, whereas in E. farinosa mortality lagged 1 year, resulting in contrasting slope-wash mortality patterns. 50% of newly established shrubs died within 5, 5, and 18 years for E. frutescens, E. farinosa, and A. salsola, respectively. The 90% mortality ages were 26 years for E. frutescens, 24 years for E. farinosa, and 51 years for A. salsola. While maximum life expectancies are unknown, estimated maximum life expectancies were 56, 66, and 86 years for E. frutescens, E. farinosa, and A. salsola, respectively. Overall, as the climate has become more arid over the past four decades, the populations in both slope and wash habitats have exhibited similar responses: reduced shrub abundances and reduced total supportable leaf areas.

De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.

Science.gov (United States)

Katona, Robert L

2015-02-01

Mammalian artificial chromosomes (MACs) are non-integrating, autonomously replicating natural chromosome-based vectors that may carry a vast amount of genetic material, which in turn enable potentially prolonged, safe, and regulated therapeutic transgene expression and render MACs as attractive genetic vectors for "gene replacement" or for controlling differentiation pathways in target cells. Satellite-DNA-based artificial chromosomes (SATACs) can be made by induced de novo chromosome formation in cells of different mammalian and plant species. These artificially generated accessory chromosomes are composed of predictable DNA sequences, and they contain defined genetic information. SATACs have already passed a number of obstacles crucial to their further development as gene therapy vectors, including large-scale purification, transfer of purified artificial chromosomes into different cells and embryos, generation of transgenic animals and germline transmission with purified SATACs, and the tissue-specific expression of a therapeutic gene from an artificial chromosome in the milk of transgenic animals. SATACs could be used in cell therapy protocols. For these methods, the most versatile target cell would be one that was pluripotent and self-renewing to address multiple disease target cell types, thus making multilineage stem cells, such as adult derived early progenitor cells and embryonic stem cells, as attractive universal host cells.

Recent advance in DNA-based traceability and authentication of livestock meat PDO and PGI products.

Science.gov (United States)

Nicoloso, Letizia; Crepaldi, Paola; Mazza, Raffaele; Ajmone-Marsan, Paolo; Negrini, Riccardo

2013-04-01

This review updates the available molecular techniques and technologies and discusses how they can be used for traceability, food control and enforcement activities. The review also provides examples on how molecular techniques succeeded to trace back unknowns to their breeds of origin, to fingerprint single individuals and to generate evidence in court cases. The examples demonstrate the potential of the DNA based traceability techniques and explore possibilities for translating the next generation genomics tools into a food and feed control and enforcement framework.

Solar satellites

Energy Technology Data Exchange (ETDEWEB)

Poher, C.

1982-01-01

A reference system design, projected costs, and the functional concepts of a satellite solar power system (SSPS) for converting sunlight falling on solar panels of a satellite in GEO to a multi-GW beam which could be received by a rectenna on earth are outlined. Electricity transmission by microwaves has been demonstrated, and a reference design system for supplying 5 GW dc to earth was devised. The system will use either monocrystalline Si or concentrator GaAs solar cells for energy collection in GEO. Development is still needed to improve the lifespan of the cells. Currently, the cell performance degrades 50 percent in efficiency after 7-8 yr in space. Each SSPS satellite would weigh either 34,000 tons (Si) or 51,000 tons (GaAs), thereby requiring the fabrication of a heavy lift launch vehicle or a single-stage-to-orbit transport in order to minimize launch costs. Costs for the solar panels have been estimated at $500/kW using the GaAs technology, with transport costs for materials to GEO being $40/kg.

Solar satellites

Science.gov (United States)

Poher, C.

A reference system design, projected costs, and the functional concepts of a satellite solar power system (SSPS) for converting sunlight falling on solar panels of a satellite in GEO to a multi-GW beam which could be received by a rectenna on earth are outlined. Electricity transmission by microwaves has been demonstrated, and a reference design system for supplying 5 GW dc to earth was devised. The system will use either monocrystalline Si or concentrator GaAs solar cells for energy collection in GEO. Development is still needed to improve the lifespan of the cells. Currently, the cell performance degrades 50 percent in efficiency after 7-8 yr in space. Each SSPS satellite would weigh either 34,000 tons (Si) or 51,000 tons (GaAs), thereby requiring the fabrication of a heavy lift launch vehicle or a single-stage-to-orbit transport in order to minimize launch costs. Costs for the solar panels have been estimated at $500/kW using the GaAs technology, with transport costs for materials to GEO being $40/kg.

Methylation patterns of repetitive DNA sequences in germ cells of Mus musculus.

Science.gov (United States)

Sanford, J; Forrester, L; Chapman, V; Chandley, A; Hastie, N

1984-03-26

The major and the minor satellite sequences of Mus musculus were undermethylated in both sperm and oocyte DNAs relative to the amount of undermethylation observed in adult somatic tissue DNA. This hypomethylation was specific for satellite sequences in sperm DNA. Dispersed repetitive and low copy sequences show a high degree of methylation in sperm DNA; however, a dispersed repetitive sequence was undermethylated in oocyte DNA. This finding suggests a difference in the amount of total genomic DNA methylation between sperm and oocyte DNA. The methylation levels of the minor satellite sequences did not change during spermiogenesis, and were not associated with the onset of meiosis or a specific stage in sperm development.

DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA

DEFF Research Database (Denmark)

Christensen, H.; Angen, Øystein; Mutters, R.

2000-01-01

The present study was aimed at reducing the time and labour used to perform DNA-DNA hybridizations for classification of bacteria at the species level. A micro-well-format DNA hybridization method was developed and validated. DNA extractions were performed by a small-scale method and DNA...... was sheared mechanically into fragments of between 400 and 700 bases. The hybridization conditions were calibrated according to DNA similarities obtained by the spectrophotometric method using strains within the family Pasteurellaceae, Optimal conditions were obtained with 300 ng DNA added per well and bound...... by covalent attachment to NucleoLink. Hybridization was performed with 500 ng DNA, 5% (w/w) of which was labelled with photo-activatable biotin (competitive hybridization) for 2.5 h at 65 degrees C in 2 x SSC followed by stringent washing with 2 x SSC at the same temperature. The criteria for acceptance...

Targeting and tracing of specific DNA sequences with dTALEs in living cells

Science.gov (United States)

Thanisch, Katharina; Schneider, Katrin; Morbitzer, Robert; Solovei, Irina; Lahaye, Thomas; Bultmann, Sebastian; Leonhardt, Heinrich

2014-01-01

Epigenetic regulation of gene expression involves, besides DNA and histone modifications, the relative positioning of DNA sequences within the nucleus. To trace specific DNA sequences in living cells, we used programmable sequence-specific DNA binding of designer transcription activator-like effectors (dTALEs). We designed a recombinant dTALE (msTALE) with variable repeat domains to specifically bind a 19-bp target sequence of major satellite DNA. The msTALE was fused with green fluorescent protein (GFP) and stably expressed in mouse embryonic stem cells. Hybridization with a major satellite probe (3D-fluorescent in situ hybridization) and co-staining for known cellular structures confirmed in vivo binding of the GFP-msTALE to major satellite DNA present at nuclear chromocenters. Dual tracing of major satellite DNA and the replication machinery throughout S-phase showed co-localization during mid to late S-phase, directly demonstrating the late replication timing of major satellite DNA. Fluorescence bleaching experiments indicated a relatively stable but still dynamic binding, with mean residence times in the range of minutes. Fluorescently labeled dTALEs open new perspectives to target and trace DNA sequences and to monitor dynamic changes in subnuclear positioning as well as interactions with functional nuclear structures during cell cycle progression and cellular differentiation. PMID:24371265

Targeting and tracing of specific DNA sequences with dTALEs in living cells.

Science.gov (United States)

Thanisch, Katharina; Schneider, Katrin; Morbitzer, Robert; Solovei, Irina; Lahaye, Thomas; Bultmann, Sebastian; Leonhardt, Heinrich

2014-04-01

Epigenetic regulation of gene expression involves, besides DNA and histone modifications, the relative positioning of DNA sequences within the nucleus. To trace specific DNA sequences in living cells, we used programmable sequence-specific DNA binding of designer transcription activator-like effectors (dTALEs). We designed a recombinant dTALE (msTALE) with variable repeat domains to specifically bind a 19-bp target sequence of major satellite DNA. The msTALE was fused with green fluorescent protein (GFP) and stably expressed in mouse embryonic stem cells. Hybridization with a major satellite probe (3D-fluorescent in situ hybridization) and co-staining for known cellular structures confirmed in vivo binding of the GFP-msTALE to major satellite DNA present at nuclear chromocenters. Dual tracing of major satellite DNA and the replication machinery throughout S-phase showed co-localization during mid to late S-phase, directly demonstrating the late replication timing of major satellite DNA. Fluorescence bleaching experiments indicated a relatively stable but still dynamic binding, with mean residence times in the range of minutes. Fluorescently labeled dTALEs open new perspectives to target and trace DNA sequences and to monitor dynamic changes in subnuclear positioning as well as interactions with functional nuclear structures during cell cycle progression and cellular differentiation.

Diurnal, seasonal and latitudinal variations of electron temperature measured by the SROSS C2 satellite at 500 km altitude and comparison with the IRI

Directory of Open Access Journals (Sweden)

P. K. Bhuyan

2002-06-01

Full Text Available The diurnal, seasonal and latitudinal variations of electron temperature Te, measured by the SROSS C2 satellite at equatorial and the low-latitudes during the low solar activity period of 1995–1997 are investigated. The average height of the satellite was ~ 500 km and it covered the latitude belt of –31° to 34° and the longitude range of 40°–100°. Te varies between 700–800 K during night-time (20:00–04:00 LT, rises sharply during sunrise (04:00–06:00 LT to reach a level of ~ 3500 K within a couple of hours and then falls between 07:00–10:00 LT to a daytime average value of ~ 1600 K. A secondary maximum is observed around 16:00–18:00 LT in summer. Latitudinal gradients in Te have been observed during the morning enhancement and daytime hours. Comparison of measured and International Reference Ionosphere (IRI predicted electron temperature reveals that the IRI predicts nighttime Te well within ~ 100 K of observation, but at other local times, the predicted Te is less than that measured in all seasons.Key words. Ionosphere, equatorial ionosphere, plasma temperature, and density

DNA Camouflage

Science.gov (United States)

2016-01-08

1 DNA Camouflage Supplementary Information Bijan Zakeri1,2*, Timothy K. Lu1,2*, Peter A. Carr2,3* 1Department of Electrical Engineering and...ll.mit.edu). Distribution A: Public Release   2 Supplementary Figure 1 DNA camouflage with the 2-state device. (a) In the presence of Cre, DSD-2[α...10 1 + Cre 1 500 1,000 length (bp) chromatogram alignment template − Cre   4 Supplementary Figure 3 DNA camouflage with a switchable

Comparative performance of the BGISEQ-500 versus Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

DEFF Research Database (Denmark)

Mak, Sarah Siu Tze Mak; Gopalakrishnan, Shyam Sunder; Carøe, Christian

2017-01-01

on degraded DNA, then directly compared the sequencing performance and data quality of the BGISEQ-500 to the Illumina HiSeq2500 platform, on DNA extracted from eight historic and ancient dog and wolf samples. Results: The data generated was largely comparable between sequencing platforms...... difference was also observed in the mitochondrial DNA percentages recovered (p = 0.018), although we believe this is likely a stochastic effect relating to the extremely low levels of mitochondria that were sequenced from three of the samples with overall very low levels of endogenous DNA. Conclusions......: Although we acknowledge our analyses were limited to animal material, our observations suggest that the BGISEQ-500 holds the potential to represent valid and potentially valuable alternative platform for palaeogenomic data generation, that is worthy of future exploration by those interested...

New insights into Trypanosoma cruzi evolution, genotyping and molecular diagnostics from satellite DNA sequence analysis.

Directory of Open Access Journals (Sweden)

Juan C Ramírez

2017-12-01

Full Text Available Trypanosoma cruzi has been subdivided into seven Discrete Typing Units (DTUs, TcI-TcVI and Tcbat. Two major evolutionary models have been proposed to explain the origin of hybrid lineages, but while it is widely accepted that TcV and TcVI are the result of genetic exchange between TcII and TcIII strains, the origin of TcIII and TcIV is still a matter of debate. T. cruzi satellite DNA (SatDNA, comprised of 195 bp units organized in tandem repeats, from both TcV and TcVI stocks were found to have SatDNA copies type TcI and TcII; whereas contradictory results were observed for TcIII stocks and no TcIV sequence has been analyzed yet. Herein, we have gone deeper into this matter analyzing 335 distinct SatDNA sequences from 19 T. cruzi stocks representative of DTUs TcI-TcVI for phylogenetic inference. Bayesian phylogenetic tree showed that all sequences were grouped in three major clusters, which corresponded to sequences from DTUs TcI/III, TcII and TcIV; whereas TcV and TcVI stocks had two sets of sequences distributed into TcI/III and TcII clusters. As expected, the lowest genetic distances were found between TcI and TcIII, and between TcV and TcVI sequences; whereas the highest ones were observed between TcII and TcI/III, and among TcIV sequences and those from the remaining DTUs. In addition, signature patterns associated to specific T. cruzi lineages were identified and new primers that improved SatDNA-based qPCR sensitivity were designed. Our findings support the theory that TcIII is not the result of a hybridization event between TcI and TcII, and that TcIV had an independent origin from the other DTUs, contributing to clarifying the evolutionary history of T. cruzi lineages. Moreover, this work opens the possibility of typing samples from Chagas disease patients with low parasitic loads and improving molecular diagnostic methods of T. cruzi infection based on SatDNA sequence amplification.

New insights into Trypanosoma cruzi evolution, genotyping and molecular diagnostics from satellite DNA sequence analysis.

Science.gov (United States)

Ramírez, Juan C; Torres, Carolina; Curto, María de Los A; Schijman, Alejandro G

2017-12-01

Trypanosoma cruzi has been subdivided into seven Discrete Typing Units (DTUs), TcI-TcVI and Tcbat. Two major evolutionary models have been proposed to explain the origin of hybrid lineages, but while it is widely accepted that TcV and TcVI are the result of genetic exchange between TcII and TcIII strains, the origin of TcIII and TcIV is still a matter of debate. T. cruzi satellite DNA (SatDNA), comprised of 195 bp units organized in tandem repeats, from both TcV and TcVI stocks were found to have SatDNA copies type TcI and TcII; whereas contradictory results were observed for TcIII stocks and no TcIV sequence has been analyzed yet. Herein, we have gone deeper into this matter analyzing 335 distinct SatDNA sequences from 19 T. cruzi stocks representative of DTUs TcI-TcVI for phylogenetic inference. Bayesian phylogenetic tree showed that all sequences were grouped in three major clusters, which corresponded to sequences from DTUs TcI/III, TcII and TcIV; whereas TcV and TcVI stocks had two sets of sequences distributed into TcI/III and TcII clusters. As expected, the lowest genetic distances were found between TcI and TcIII, and between TcV and TcVI sequences; whereas the highest ones were observed between TcII and TcI/III, and among TcIV sequences and those from the remaining DTUs. In addition, signature patterns associated to specific T. cruzi lineages were identified and new primers that improved SatDNA-based qPCR sensitivity were designed. Our findings support the theory that TcIII is not the result of a hybridization event between TcI and TcII, and that TcIV had an independent origin from the other DTUs, contributing to clarifying the evolutionary history of T. cruzi lineages. Moreover, this work opens the possibility of typing samples from Chagas disease patients with low parasitic loads and improving molecular diagnostic methods of T. cruzi infection based on SatDNA sequence amplification.

Effects of radiation on DNA

International Nuclear Information System (INIS)

Medina, V.F.O.

1978-01-01

Irradiation has been shown to depress DNA (deoxyribonucleic acid) synthesis resulting in deficient DNA synthesis. In one experiment, Hela S 3 cells completed the next division after a dose of 500 rads to 200 kw X-rays. Another experiment showed that the amount of DNA synthesized was dependent on the stage in the generation cycle at which the cells are irradiated (Giffites and Tolmach, 1975). DNA synthesis was measured by radioactive thymidine incorporation. The smallest deficiency (20-35%) after a dose of 500 rad X-ray was observed in Hela S 3 cells irradiated in early G 1 or early G 2 , while the greatest deficiency (55-70*) after 500 rad X-ray was found in cells irradiated at mitosis or at the Gsub(1)/S transition. Using velocity sedimentation in alkaline gradients of the DNA from hamster, Elkind, et al 1972, studied repair processes as a function of X-ray dose. DNA containing material released by alkaline lysis was found initially contained in a complex-containing lipid, the sedimentation of which was anomalous relative to denatured RNA from unirradated cells. Doses of X-rays small enough to be in the range that permits high survival (100-800 rads) speed the resolution of single-stranded DNA from this DNA complex, giving rise to a species having a number average molecular weight of 2 x 10 8 daltons. Larger doses greater than 1000 to 2000 rads resulted in a degradation of these DNA strands. Incubation after irradiation resulted in the rapid repair of damage, although the rate of repair of damage to the complex resulted in a reassociation of lipid and DNA. This evidence supports the possibility that a large DNA-membrane structure is a principal target of radiation

Transcription of tandemly repetitive DNA: functional roles.

Science.gov (United States)

Biscotti, Maria Assunta; Canapa, Adriana; Forconi, Mariko; Olmo, Ettore; Barucca, Marco

2015-09-01

A considerable fraction of the eukaryotic genome is made up of satellite DNA constituted of tandemly repeated sequences. These elements are mainly located at centromeres, pericentromeres, and telomeres and are major components of constitutive heterochromatin. Although originally satellite DNA was thought silent and inert, an increasing number of studies are providing evidence on its transcriptional activity supporting, on the contrary, an unexpected dynamicity. This review summarizes the multiple structural roles of satellite noncoding RNAs at chromosome level. Indeed, satellite noncoding RNAs play a role in the establishment of a heterochromatic state at centromere and telomere. These highly condensed structures are indispensable to preserve chromosome integrity and genome stability, preventing recombination events, and ensuring the correct chromosome pairing and segregation. Moreover, these RNA molecules seem to be involved also in maintaining centromere identity and in elongation, capping, and replication of telomere. Finally, the abnormal variation of centromeric and pericentromeric DNA transcription across major eukaryotic lineages in stress condition and disease has evidenced the critical role that these transcripts may play and the potentially dire consequences for the organism.

Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration.

Science.gov (United States)

Hatazawa, Yukino; Ono, Yusuke; Hirose, Yuma; Kanai, Sayaka; Fujii, Nobuharu L; Machida, Shuichi; Nishino, Ichizo; Shimizu, Takahiko; Okano, Masaki; Kamei, Yasutomi; Ogawa, Yoshihiro

2018-03-01

DNA methylation is an epigenetic mechanism regulating gene expression. In this study, we observed that DNA methyltransferase 3a (Dnmt3a) expression is decreased after muscle atrophy. We made skeletal muscle-specific Dnmt3a-knockout (Dnmt3a-KO) mice. The regeneration capacity after muscle injury was markedly decreased in Dnmt3a-KO mice. Diminished mRNA and protein expression of Dnmt3a were observed in skeletal muscles as well as in satellite cells, which are important for muscle regeneration, in Dnmt3a-KO mice. Dnmt3a-KO satellite cell showed smaller in size (length/area), suggesting suppressed myotube differentiation. Microarray analysis of satellite cells showed that expression of growth differentiation factor 5 (Gdf5) mRNA was markedly increased in Dnmt3a-KO mice. The DNA methylation level of the Gdf5 promoter was markedly decreased in Dnmt3a-KO satellite cells. In addition, DNA methylation inhibitor azacytidine treatment increased Gdf5 expression in wild-type satellite cells, suggesting Gdf5 expression is regulated by DNA methylation. Also, we observed increased inhibitor of differentiation (a target of Gdf5) mRNA expression in Dnmt3a-KO satellite cells. Thus, Dnmt3a appears to regulate satellite cell differentiation via DNA methylation. This mechanism may play a role in the decreased regeneration capacity during atrophy such as in aged sarcopenia.-Hatazawa, Y., Ono, Y., Hirose, Y., Kanai, S., Fujii, N. L., Machida, S., Nishino, I., Shimizu, T., Okano, M., Kamei, Y., Ogawa, Y. Reduced Dnmt3a increases Gdf5 expression with suppressed satellite cell differentiation and impaired skeletal muscle regeneration.

DNA Methylation at a Bovine Alpha Satellite I Repeat CpG Site during Development following Fertilization and Somatic Cell Nuclear Transfer

OpenAIRE

Couldrey, Christine; Wells, David N.

2013-01-01

Incomplete epigenetic reprogramming is postulated to contribute to the low developmental success following somatic cell nuclear transfer (SCNT). Here, we describe the epigenetic reprogramming of DNA methylation at an alpha satellite I CpG site (αsatI-5) during development of cattle generated either by artificial insemination (AI) or in vitro fertilization (IVF) and SCNT. Quantitative methylation analysis identified that SCNT donor cells were highly methylated at αsatI-5 and resulting SCNT bla...

Methylation patterns of repetitive DNA sequences in germ cells of Mus musculus.

OpenAIRE

Sanford, J; Forrester, L; Chapman, V; Chandley, A; Hastie, N

1984-01-01

The major and the minor satellite sequences of Mus musculus were undermethylated in both sperm and oocyte DNAs relative to the amount of undermethylation observed in adult somatic tissue DNA. This hypomethylation was specific for satellite sequences in sperm DNA. Dispersed repetitive and low copy sequences show a high degree of methylation in sperm DNA; however, a dispersed repetitive sequence was undermethylated in oocyte DNA. This finding suggests a difference in the amount of total genomic...

A general theory for the Uranian satellites

Science.gov (United States)

Laskar, J.

1986-01-01

A general analytical theory of the five main satellites of Uranus, including the secular and short period terms hereafter denoted by GUST, is presented. A comparison is made with an internal numerical integration with nominal masses of Veillet (1983). The precision of the theory goes from about 10 km for Miranda to 100 km for Oberon. The short period terms in the motions of Titania and Oberon are larger than 500 km. They should make possible the determination of the masses of the outer satellites through the optical data of Voyager encounter.

Transcription of highly repetitive tandemly organized DNA in amphibians and birds: A historical overview and modern concepts.

Science.gov (United States)

Trofimova, Irina; Krasikova, Alla

2016-12-01

Tandemly organized highly repetitive DNA sequences are crucial structural and functional elements of eukaryotic genomes. Despite extensive evidence, satellite DNA remains an enigmatic part of the eukaryotic genome, with biological role and significance of tandem repeat transcripts remaining rather obscure. Data on tandem repeats transcription in amphibian and avian model organisms is fragmentary despite their genomes being thoroughly characterized. Review systematically covers historical and modern data on transcription of amphibian and avian satellite DNA in somatic cells and during meiosis when chromosomes acquire special lampbrush form. We highlight how transcription of tandemly repetitive DNA sequences is organized in interphase nucleus and on lampbrush chromosomes. We offer LTR-activation hypotheses of widespread satellite DNA transcription initiation during oogenesis. Recent explanations are provided for the significance of high-yield production of non-coding RNA derived from tandemly organized highly repetitive DNA. In many cases the data on the transcription of satellite DNA can be extrapolated from lampbrush chromosomes to interphase chromosomes. Lampbrush chromosomes with applied novel technical approaches such as superresolution imaging, chromosome microdissection followed by high-throughput sequencing, dynamic observation in life-like conditions provide amazing opportunities for investigation mechanisms of the satellite DNA transcription.

About Nano-JASMINE Satellite System and Project Status

Science.gov (United States)

Sako, Nobutada

Intelligent Space Systems Laboratory, The University of Tokyo (ISSL) and National Astronomical Observatory of Japan (NAO) have been developing a small infrared astrometry satellite named “Nano-JASMINE”. The satellite size is about 50cm cubic and 20kg, which plays a pre-cursor role of JASMINE Project which is programmed by NAO and JAXA. In addition, since there has been only one astrometry satellite HIPPARCOS by ESA in the past, Nano-JASMINE is also expected to achieve certain scientific results in the field of astrometry. In this project, ISSL aims to develop new advanced small satellite bus system whose performance is comparable to that of 100-500kg sized satellites, including attitude stability of 1 arc-second and thermal stability of the mission subsystem of 1 mK. This paper overviews the Nano-JASMINE bus system with emphasis on attitude and thermal control systems.

Regenerative capacity of old muscle stem cells declines without significant accumulation of DNA damage.

Directory of Open Access Journals (Sweden)

Wendy Cousin

Full Text Available The performance of adult stem cells is crucial for tissue homeostasis but their regenerative capacity declines with age, leading to failure of multiple organs. In skeletal muscle this failure is manifested by the loss of functional tissue, the accumulation of fibrosis, and reduced satellite cell-mediated myogenesis in response to injury. While recent studies have shown that changes in the composition of the satellite cell niche are at least in part responsible for the impaired function observed with aging, little is known about the effects of aging on the intrinsic properties of satellite cells. For instance, their ability to repair DNA damage and the effects of a potential accumulation of DNA double strand breaks (DSBs on their regenerative performance remain unclear. This work demonstrates that old muscle stem cells display no significant accumulation of DNA DSBs when compared to those of young, as assayed after cell isolation and in tissue sections, either in uninjured muscle or at multiple time points after injury. Additionally, there is no significant difference in the expression of DNA DSB repair proteins or globally assayed DNA damage response genes, suggesting that not only DNA DSBs, but also other types of DNA damage, do not significantly mark aged muscle stem cells. Satellite cells from DNA DSB-repair-deficient SCID mice do have an unsurprisingly higher level of innate DNA DSBs and a weakened recovery from gamma-radiation-induced DNA damage. Interestingly, they are as myogenic in vitro and in vivo as satellite cells from young wild type mice, suggesting that the inefficiency in DNA DSB repair does not directly correlate with the ability to regenerate muscle after injury. Overall, our findings suggest that a DNA DSB-repair deficiency is unlikely to be a key factor in the decline in muscle regeneration observed upon aging.

Fast phylogenetic DNA barcoding

DEFF Research Database (Denmark)

Terkelsen, Kasper Munch; Boomsma, Wouter Krogh; Willerslev, Eske

2008-01-01

We present a heuristic approach to the DNA assignment problem based on phylogenetic inferences using constrained neighbour joining and non-parametric bootstrapping. We show that this method performs as well as the more computationally intensive full Bayesian approach in an analysis of 500 insect...... DNA sequences obtained from GenBank. We also analyse a previously published dataset of environmental DNA sequences from soil from New Zealand and Siberia, and use these data to illustrate the fact that statistical approaches to the DNA assignment problem allow for more appropriate criteria...... for determining the taxonomic level at which a particular DNA sequence can be assigned....

Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry

DEFF Research Database (Denmark)

Vranken, Charlotte; Deen, Jochem; Dirix, Lieve

2014-01-01

We demonstrate an approach to optical DNA mapping, which enables near single-molecule characterization of whole bacteriophage genomes. Our approach uses a DNA methyltransferase enzyme to target labelling to specific sites and copper-catalysed azide-alkyne cycloaddition to couple a fluorophore...... to the DNA. We achieve a labelling efficiency of ∼70% with an average labelling density approaching one site every 500 bp. Such labelling density bridges the gap between the output of a typical DNA sequencing experiment and the long-range information derived from traditional optical DNA mapping. We lay...... the foundations for a wider-scale adoption of DNA mapping by screening 11 methyltransferases for their ability to direct sequence-specific DNA transalkylation; the first step of the DNA labelling process and by optimizing reaction conditions for fluorophore coupling via a click reaction. Three of 11 enzymes...

Shallow-earth rheology from glacial isostasy and satellite gravity : A sensitivity analysis for GOCE

NARCIS (Netherlands)

Schotman, H.H.A.

2008-01-01

In recent years, satellite gravity missions have been launched that probe the earth's long- to mediumwavelength (1000 - 500 km) gravity field. The upcoming ESA satellite gravity mission GOCE is predicted to measure the gravity field with an accuracy of a few centimeters at spatial scales of 100 km.

Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression.

Science.gov (United States)

Aze, Antoine; Sannino, Vincenzo; Soffientini, Paolo; Bachi, Angela; Costanzo, Vincenzo

2016-06-01

Half of the human genome is made up of repetitive DNA. However, mechanisms underlying replication of chromosome regions containing repetitive DNA are poorly understood. We reconstituted replication of defined human chromosome segments using bacterial artificial chromosomes in Xenopus laevis egg extract. Using this approach we characterized the chromatin assembly and replication dynamics of centromeric alpha-satellite DNA. Proteomic analysis of centromeric chromatin revealed replication-dependent enrichment of a network of DNA repair factors including the MSH2-6 complex, which was required for efficient centromeric DNA replication. However, contrary to expectations, the ATR-dependent checkpoint monitoring DNA replication fork arrest could not be activated on highly repetitive DNA due to the inability of the single-stranded DNA binding protein RPA to accumulate on chromatin. Electron microscopy of centromeric DNA and supercoil mapping revealed the presence of topoisomerase I-dependent DNA loops embedded in a protein matrix enriched for SMC2-4 proteins. This arrangement suppressed ATR signalling by preventing RPA hyper-loading, facilitating replication of centromeric DNA. These findings have important implications for our understanding of repetitive DNA metabolism and centromere organization under normal and stressful conditions.

Research of the key technology in satellite communication networks

Science.gov (United States)

Zeng, Yuan

2018-02-01

According to the prediction, in the next 10 years the wireless data traffic will be increased by 500-1000 times. Not only the wireless data traffic will be increased exponentially, and the demand for diversified traffic will be increased. Higher requirements for future mobile wireless communication system had brought huge market space for satellite communication system. At the same time, the space information networks had been greatly developed with the depth of human exploration of space activities, the development of space application, the expansion of military and civilian application. The core of spatial information networks is the satellite communication. The dissertation presented the communication system architecture, the communication protocol, the routing strategy, switch scheduling algorithm and the handoff strategy based on the satellite communication system. We built the simulation platform of the LEO satellites networks and simulated the key technology using OPNET.

Seasonal to Interannual Variability of Satellite-Based Precipitation Estimates in the Pacific Ocean Associated with ENSO from 1998 to 2014

Directory of Open Access Journals (Sweden)

Xueyan Hou

2016-10-01

Full Text Available Based on a widely used satellite precipitation product (TRMM Multi-satellite Precipitation Analysis 3B43, we analyzed the spatiotemporal variability of precipitation over the Pacific Ocean for 1998–2014 at seasonal and interannual timescales, separately, using the conventional empirical orthogonal function (EOF and investigated the seasonal patterns associated with El Niño–Southern Oscillation (ENSO cycles using season-reliant empirical orthogonal function (SEOF analysis. Lagged correlation analysis was also applied to derive the lead/lag correlations of the first two SEOF modes for precipitation with Pacific Decadal Oscillation (PDO and two types of El Niño, i.e., central Pacific (CP El Niño and eastern Pacific (EP El Niño. We found that: (1 The first two seasonal EOF modes for precipitation represent the annual cycle of precipitation variations for the Pacific Ocean and the first interannual EOF mode shows the spatiotemporal variability associated with ENSO; (2 The first SEOF mode for precipitation is simultaneously associated with the development of El Niño and most likely coincides with CP El Niño. The second SEOF mode lagged behind ENSO by one year and is associated with post-El Niño years. PDO modulates precipitation variability significantly only when ENSO occurs by strengthening and prolonging the impacts of ENSO; (3 Seasonally evolving patterns of the first two SEOF modes represent the consecutive precipitation patterns associated with the entire development of EP El Niño and the following recovery year. The most significant variation occurs over the tropical Pacific, especially in the Intertropical Convergence Zone (ITCZ and South Pacific Convergence Zone (SPCZ; (4 Dry conditions in the western basin of the warm pool and wet conditions along the ITCZ and SPCZ bands during the mature phase of El Niño are associated with warm sea surface temperatures in the central tropical Pacific, and a subtropical anticyclone dominating

Molecular biology of fuselloviruses and their satellites

DEFF Research Database (Denmark)

Contursi, Patrizia; Fusco, Salvatore; Cannio, Raffaele

2014-01-01

Fuselloviruses, also known as Sulfolobus Spindle-shaped viruses (SSVs), are "lemon"- or "spindle"-shaped double-stranded DNA viruses. Among them, SSV1, SSV2 and the satellite viruses pSSVx and pSSVi have been investigated at the structural, genetic, transcriptomic, proteomic and biochemical levels...

The 30/20 GHz communications satellite trunking network study

Science.gov (United States)

Kolb, W.

1981-01-01

Alternative transmission media for a CONUS-wide trunking network in the years 1990 and 2000 are examined. The alternative technologies comprised fiber optic cable, conventional C- and Ku-band satellites, and 30/20 GHz satellites. Three levels of implementation were considered - a 10-city network, a 20-city network, and a 40-city network. The cities selected were the major metropolitan areas with the greatest communications demand. All intercity voice, data, and video traffic carried more than 40 miles was included in the analysis. In the optimized network, traffic transmitted less than 500 miles was found to be better served by fiber optic cable in 1990. By the year 2000, the crossover point would be down to 200 miles, assuming availability of 30/20 GHz satellites.

Characterization of a chromosome-specific chimpanzee alpha satellite subset: Evolutionary relationship to subsets on human chromosomes

Energy Technology Data Exchange (ETDEWEB)

Warburton, P.E.; Gosden, J.; Lawson, D. [Western General Hospital, Edinburgh (United Kingdom)] [and others

1996-04-15

Alpha satellite DNA is a tandemly repeated DNA family found at the centromeres of all primate chromosomes examined. The fundamental repeat units of alpha satellite DNA are diverged 169- to 172-bp monomers, often found to be organized in chromosome-specific higher-order repeat units. The chromosomes of human (Homo sapiens (HSA)), chimpanzee (Pan troglodytes (PTR) and Pan paniscus), and gorilla (Gorilla gorilla) share a remarkable similarity and synteny. It is of interest to ask if alpha satellite arrays at centromeres of homologous chromosomes between these species are closely related (evolving in an orthologous manner) or if the evolutionary processes that homogenize and spread these arrays within and between chromosomes result in nonorthologous evolution of arrays. By using PCR primers specific for human chromosome 17-specific alpha satellite DNA, we have amplified, cloned, and characterized a chromosome-specific subset from the PTR chimpanzee genome. Hybridization both on Southern blots and in situ as well as sequence analysis show that this subset is most closely related, as expected, to sequences on HSA 17. However, in situ hybridization reveals that this subset is not found on the homologous chromosome in chimpanzee (PTR 19), but instead on PTR 12, which is homologous to HSA 2p. 40 refs., 3 figs.

Evaluating Surface Radiation Fluxes Observed From Satellites in the Southeastern Pacific Ocean

Science.gov (United States)

Pinker, R. T.; Zhang, B.; Weller, R. A.; Chen, W.

2018-03-01

This study is focused on evaluation of current satellite and reanalysis estimates of surface radiative fluxes in a climatically important region. It uses unique observations from the STRATUS Ocean Reference Station buoy in a region of persistent marine stratus clouds 1,500 km off northern Chile during 2000-2012. The study shows that current satellite estimates are in better agreement with buoy observations than model outputs at a daily time scale and that satellite data depict well the observed annual cycle in both shortwave and longwave surface radiative fluxes. Also, buoy and satellite estimates do not show any significant trend over the period of overlap or any interannual variability. This verifies the stability and reliability of the satellite data and should make them useful to examine El Niño-Southern Oscillation variability influences on surface radiative fluxes at the STRATUS site for longer periods for which satellite record is available.

Satellite based wind resource assessment over the South China Sea

DEFF Research Database (Denmark)

Badger, Merete; Astrup, Poul; Hasager, Charlotte Bay

2014-01-01

variations are clearly visible across the domain; for instance sheltering effects caused by the land masses. The satellite based wind resource maps have two shortcomings. One is the lack of information at the higher vertical levels where wind turbines operate. The other is the limited number of overlapping...... years of WRF data – specifically the parameters heat flux, air temperature, and friction velocity – are used to calculate a long-term correction for atmospheric stability effects. The stability correction is applied to the satellite based wind resource maps together with a vertical wind profile...... from satellite synthetic aperture radar (SAR) data are particularly suitable for offshore wind energy applications because they offer a spatial resolution up to 500 m and include coastal seas. In this presentation, satellite wind maps are used in combination with mast observations and numerical...

A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity.

Science.gov (United States)

Sina, Abu Ali Ibn; Foster, Matthew Thomas; Korbie, Darren; Carrascosa, Laura G; Shiddiky, Muhammad J A; Gao, Jing; Dey, Shuvashis; Trau, Matt

2017-10-07

We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl -1 of DNA with no sequencing requirement.

Interspersion of highly repetitive DNA with single copy DNA in the genome of the red crab, Geryon quinquedens

Energy Technology Data Exchange (ETDEWEB)

Christie, N.T. (Univ. of Tennessee, Oak Ridge); Skinner, D.M.

1979-02-01

Kinetic analysis of the reassociation of 420 nucleotide (NT) long fragments has shown that essentially all of the repetitive sequences of the DNA of the red crab Geryon quinquedens are highly repetitive. There are negligible amounts of low and intermediate repetitive DNAs. Though atypical of most eukaryotes, this pattern has been observed in al other brachyurans (true crabs) studied. The major repetitive component is subdivided into short runs of 300 NT and longer runs of greater than 1200 NT while the minor component has an average sequence length of 400 NT. Both components reassociate at rates commonly observed for satellite DNAs. Unique among eukaryotes the organization of the genome includes single copy DNA contiguous to short runs (300 NT) of both repetitive components. Although patent satellites are not present, subsets of the repetitive DNA have been isolated by either restriction endonuclease digestion or by centrifugation in Ag/sup +/ or Hg/sup 2 +//Cs/sub 2/SO/sub 4/ density gradients.

More efficient repair of DNA double-strand breaks in skeletal muscle stem cells compared to their committed progeny

Directory of Open Access Journals (Sweden)

Leyla Vahidi Ferdousi

2014-11-01

Full Text Available The loss of genome integrity in adult stem cells results in accelerated tissue aging and is possibly cancerogenic. Adult stem cells in different tissues appear to react robustly to DNA damage. We report that adult skeletal stem (satellite cells do not primarily respond to radiation-induced DNA double-strand breaks (DSBs via differentiation and exhibit less apoptosis compared to other myogenic cells. Satellite cells repair these DNA lesions more efficiently than their committed progeny. Importantly, non-proliferating satellite cells and post-mitotic nuclei in the fiber exhibit dramatically distinct repair efficiencies. Altogether, reduction of the repair capacity appears to be more a function of differentiation than of the proliferation status of the muscle cell. Notably, satellite cells retain a high efficiency of DSB repair also when isolated from the natural niche. Finally, we show that repair of DSB substrates is not only very efficient but, surprisingly, also very accurate in satellite cells and that accurate repair depends on the key non-homologous end-joining factor DNA-PKcs.

More efficient repair of DNA double-strand breaks in skeletal muscle stem cells compared to their committed progeny.

Science.gov (United States)

Vahidi Ferdousi, Leyla; Rocheteau, Pierre; Chayot, Romain; Montagne, Benjamin; Chaker, Zayna; Flamant, Patricia; Tajbakhsh, Shahragim; Ricchetti, Miria

2014-11-01

The loss of genome integrity in adult stem cells results in accelerated tissue aging and is possibly cancerogenic. Adult stem cells in different tissues appear to react robustly to DNA damage. We report that adult skeletal stem (satellite) cells do not primarily respond to radiation-induced DNA double-strand breaks (DSBs) via differentiation and exhibit less apoptosis compared to other myogenic cells. Satellite cells repair these DNA lesions more efficiently than their committed progeny. Importantly, non-proliferating satellite cells and post-mitotic nuclei in the fiber exhibit dramatically distinct repair efficiencies. Altogether, reduction of the repair capacity appears to be more a function of differentiation than of the proliferation status of the muscle cell. Notably, satellite cells retain a high efficiency of DSB repair also when isolated from the natural niche. Finally, we show that repair of DSB substrates is not only very efficient but, surprisingly, also very accurate in satellite cells and that accurate repair depends on the key non-homologous end-joining factor DNA-PKcs. Copyright © 2014. Published by Elsevier B.V.

Human Artificial Chromosomes with Alpha Satellite-Based De Novo Centromeres Show Increased Frequency of Nondisjunction and Anaphase Lag

OpenAIRE

Rudd, M. Katharine; Mays, Robert W.; Schwartz, Stuart; Willard, Huntington F.

2003-01-01

Human artificial chromosomes have been used to model requirements for human chromosome segregation and to explore the nature of sequences competent for centromere function. Normal human centromeres require specialized chromatin that consists of alpha satellite DNA complexed with epigenetically modified histones and centromere-specific proteins. While several types of alpha satellite DNA have been used to assemble de novo centromeres in artificial chromosome assays, the extent to which they fu...

The Delta low-inclination satellite concept, an opportunity to enhance the science return of the Swarm mission

DEFF Research Database (Denmark)

Hulot, Gauthier; Leger, Jean-Michel; Olsen, Nils

ESA’s Swarm mission aims at studying all sources of Earth’s magnetic field. It consists of two satellites (Alpha and Charlie), which fly side-by-side on near polar orbits at an altitude of slightly less than 500 km, and of a third satellite (Bravo) on a similar but slightly more polar and higher ...

Evaluation of DNA bending models in their capacity to predict electrophoretic migration anomalies of satellite DNA sequences

Czech Academy of Sciences Publication Activity Database

Matyášek, Roman; Fulneček, Jaroslav; Kovařík, Aleš

2013-01-01

Roč. 34, č. 17 (2013), s. 2511-2521 ISSN 0173-0835 R&D Projects: GA ČR(CZ) GA206/09/1751; GA ČR(CZ) GAP501/10/0208; GA ČR(CZ) GA13-10057S Institutional research plan: CEZ:AV0Z50040702 Institutional support: RVO:68081707 Keywords : HIGHLY REPETITIVE DNA * DOUBLE-HELICAL DNA * CURVED DNA Subject RIV: AC - Archeology, Anthropology, Ethnology Impact factor: 3.161, year: 2013

Determining the Cost Effectiveness of Nano-Satellites

Science.gov (United States)

2014-09-01

purchased as little as $7,500 ( Pumpkin 2014) for academic focused missions. Traditional satellites often cost hundreds of millions of dollars. For...and was not easily modified. The costs for the six-year scenario were only spread over five years. If the cost model were updated to spread the...files/national_space_policy_6-28-10.pdf. Pumpkin . “ Pumpkin Price List.” Pumpkin . May 23, 2014. http://www.pumpkininc.com/content/doc/forms

Human artificial chromosomes with alpha satellite-based de novo centromeres show increased frequency of nondisjunction and anaphase lag.

Science.gov (United States)

Rudd, M Katharine; Mays, Robert W; Schwartz, Stuart; Willard, Huntington F

2003-11-01

Human artificial chromosomes have been used to model requirements for human chromosome segregation and to explore the nature of sequences competent for centromere function. Normal human centromeres require specialized chromatin that consists of alpha satellite DNA complexed with epigenetically modified histones and centromere-specific proteins. While several types of alpha satellite DNA have been used to assemble de novo centromeres in artificial chromosome assays, the extent to which they fully recapitulate normal centromere function has not been explored. Here, we have used two kinds of alpha satellite DNA, DXZ1 (from the X chromosome) and D17Z1 (from chromosome 17), to generate human artificial chromosomes. Although artificial chromosomes are mitotically stable over many months in culture, when we examined their segregation in individual cell divisions using an anaphase assay, artificial chromosomes exhibited more segregation errors than natural human chromosomes (P artificial chromosomes missegregate over a fivefold range, the data suggest that variable centromeric DNA content and/or epigenetic assembly can influence the mitotic behavior of artificial chromosomes.

Detecting the Immune System Response of a 500 Year-Old Inca Mummy

Science.gov (United States)

Corthals, Angelique; Koller, Antonius; Martin, Dwight W.; Rieger, Robert; Chen, Emily I.; Bernaski, Mario; Recagno, Gabriella; Dávalos, Liliana M.

2012-01-01

Disease detection in historical samples currently relies on DNA extraction and amplification, or immunoassays. These techniques only establish pathogen presence rather than active disease. We report the first use of shotgun proteomics to detect the protein expression profile of buccal swabs and cloth samples from two 500-year-old Andean mummies. The profile of one of the mummies is consistent with immune system response to severe pulmonary bacterial infection at the time of death. Presence of a probably pathogenic Mycobacterium sp. in one buccal swab was confirmed by DNA amplification, sequencing, and phylogenetic analyses. Our study provides positive evidence of active pathogenic infection in an ancient sample for the first time. The protocol introduced here is less susceptible to contamination than DNA-based or immunoassay-based studies. In scarce forensic samples, shotgun proteomics narrows the range of pathogens to detect using DNA assays, reducing cost. This analytical technique can be broadly applied for detecting infection in ancient samples to answer questions on the historical ecology of specific pathogens, as well as in medico-legal cases when active pathogenic infection is suspected. PMID:22848450

Search for shot-time growths of flares od cosmic heavy nuclei according to measurement data at ''Prognoz'' satellites

International Nuclear Information System (INIS)

Volodichev, N.N.; Savenko, I.A.; Suslov, A.A.

1983-01-01

Surch for short-time growths of fluxes of mainly cosmic heavy nuclei with the energy epsilon > or approximately 500 MeV/nucleon according to measurement data at ''Prognoz-2'' and ''Prognoz-3'' satellites is undertaken. Such growths have been recorded during the flights of the first soviet cosmic rockets, spacecraft-satellites, ''Electron'', ''Molnia-1'' satellites. At the ''Prognoz'' satellite such growth have not been observed. Moreover, the 2.1.1974 growth found at the ''Molnia-1'' satellite by the telescope of scintillation and Cherenkov counters has not been recorded by the analogous device at ''Prognoz-3'' satellite. Therefore, the problem on the nature of short-time growths of the heavy nuclei fluxes remains unsolved

More efficient repair of DNA double-strand breaks in skeletal muscle stem cells compared to their committed progeny

OpenAIRE

Leyla Vahidi Ferdousi; Pierre Rocheteau; Romain Chayot; Benjamin Montagne; Zayna Chaker; Patricia Flamant; Shahragim Tajbakhsh; Miria Ricchetti

2014-01-01

International audience; The loss of genome integrity in adult stem cells results in accelerated tissue aging and is possibly cancerogenic. Adult stem cells in different tissues appear to react robustly to DNA damage. We report that adult skeletal stem (satellite) cells do not primarily respond to radiation-induced DNA double-strand breaks (DSBs) via differentiation and exhibit less apoptosis compared to other myogenic cells. Satellite cells repair these DNA lesions more efficiently than their...

Oman: World Oil Report 1991

International Nuclear Information System (INIS)

Anon.

1991-01-01

This paper reports that for the sixth consecutive year, Oman should retain its title as the biggest driller in the Middle East in 1991. An accelerated program in 1990 pushed production to an all-time record 700,000 bpd late in the year. Although not a member of Opec, Oman has cooperated with the group in restraining output as needed to support oil prices. Petroleum Development Oman (PDO), a partnership of the government (60%), Royal Dutch Shell (34%), Total (4%) and Partex (2%), remains by far the biggest producer. This year, PDO will begin work on its $500-million effort to boost production from its Lekhwair field from a current 24,000 bpd to 110,000 bpd by 1994. Last year, PDO also drilled 15 horizontal wells, most of which were successful in increasing per well production compared to conventional vertical holes. The horizontal program has been continued this year with two rings

Boom-Bust Turnovers of Megabase-Sized Centromeric DNA in Solanum Species: Rapid Evolution of DNA Sequences Associated with Centromeres

Czech Academy of Sciences Publication Activity Database

Zhang, H.Q.; Koblížková, Andrea; Wang, K.; Gong, Z.Y.; Oliveira, L.; Torres, G.A.; Wu, Y.; Zhang, W.; Novák, Petr; Buell, C.R.; Macas, Jiří; Jiang, J.

2014-01-01

Roč. 26, č. 4 (2014), s. 1436-1447 ISSN 1040-4651 Institutional support: RVO:60077344 Keywords : Alpha-satellite DNA * repetitive sequences * rice centromeres Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 9.338, year: 2014

Molecular cloning and characterization of satellite DNA sequences from constitutive heterochromatin of the habu snake (Protobothrops flavoviridis, Viperidae) and the Burmese python (Python bivittatus, Pythonidae).

Science.gov (United States)

Matsubara, Kazumi; Uno, Yoshinobu; Srikulnath, Kornsorn; Seki, Risako; Nishida, Chizuko; Matsuda, Yoichi

2015-12-01

Highly repetitive DNA sequences of the centromeric heterochromatin provide valuable molecular cytogenetic markers for the investigation of genomic compartmentalization in the macrochromosomes and microchromosomes of sauropsids. Here, the relationship between centromeric heterochromatin and karyotype evolution was examined using cloned repetitive DNA sequences from two snake species, the habu snake (Protobothrops flavoviridis, Crotalinae, Viperidae) and Burmese python (Python bivittatus, Pythonidae). Three satellite DNA (stDNA) families were isolated from the heterochromatin of these snakes: 168-bp PFL-MspI from P. flavoviridis and 196-bp PBI-DdeI and 174-bp PBI-MspI from P. bivittatus. The PFL-MspI and PBI-DdeI sequences were localized to the centromeric regions of most chromosomes in the respective species, suggesting that the two sequences were the major components of the centromeric heterochromatin in these organisms. The PBI-MspI sequence was localized to the pericentromeric region of four chromosome pairs. The PFL-MspI and the PBI-DdeI sequences were conserved only in the genome of closely related species, Gloydius blomhoffii (Crotalinae) and Python molurus, respectively, although their locations on the chromosomes were slightly different. In contrast, the PBI-MspI sequence was also in the genomes of P. molurus and Boa constrictor (Boidae), and additionally localized to the centromeric regions of eight chromosome pairs in B. constrictor, suggesting that this sequence originated in the genome of a common ancestor of Pythonidae and Boidae, approximately 86 million years ago. The three stDNA sequences showed no genomic compartmentalization between the macrochromosomes and microchromosomes, suggesting that homogenization of the centromeric and/or pericentromeric stDNA sequences occurred in the macrochromosomes and microchromosomes of these snakes.

Role of oxidative DNA damage in genome instability and cancer

International Nuclear Information System (INIS)

Bignami, M.; Kunkel, T.

2009-01-01

Inactivation of mismatch repair (MMR) is associated with a dramatic genomic instability that is observed experimentally as a mutator phenotype and micro satellite instability (MSI). It has been implicit that the massive genetic instability in MMR defective cells simply reflects the accumulation of spontaneous DNA polymerase errors during DNA replication. We recently identified oxidation damage, a common threat to DNA integrity to which purines are very susceptible, as an important cofactor in this genetic instability

Satellites

International Nuclear Information System (INIS)

Burns, J.A.; Matthews, M.S.

1986-01-01

The present work is based on a conference: Natural Satellites, Colloquium 77 of the IAU, held at Cornell University from July 5 to 9, 1983. Attention is given to the background and origins of satellites, protosatellite swarms, the tectonics of icy satellites, the physical characteristics of satellite surfaces, and the interactions of planetary magnetospheres with icy satellite surfaces. Other topics include the surface composition of natural satellites, the cratering of planetary satellites, the moon, Io, and Europa. Consideration is also given to Ganymede and Callisto, the satellites of Saturn, small satellites, satellites of Uranus and Neptune, and the Pluto-Charon system

Satellite-borne study of seismic phenomena by low frequency magnetic field observations

Science.gov (United States)

Schwingenschuh, Konrad; Magnes, Werner; Xuhui, Shen; Wang, Jindong; Pollinger, Andreas; Hagen, Christian; Prattes, Gustav; Eichelberger, Hans-Ulrich; Wolbang, Daniel; Boudjada, Mohammed Y.; Besser, Bruno P.; Rozhnoi, Alexander A.; Zhang, Tielong

2015-04-01

A combined scalar-vector magnetic field experiment will be flown on the upcoming CSES mission (China Seismo-Electromagnetic Satellite). Magnetic field data from DC to 30 Hz will be measured with an accuracy of about 10 pT. A fluxgate instrument will provide the 3 magnetic field components and a new type of an optically pumped magnetometer [see Pollinger, 2010] will measure the magnitude of the ambient magnetic field. The satellite will operate in a Sun synchronous polar orbit at an altitude of about 500 km and with an inclination of 97°. We present a model of magnetic field fluctuations in the upper ionosphere based on previous satellite observations and on a model of the lithospheric-atmospheric-ionospheric coupling. Pollinger et al., CDSM-a new scalar magnetometer, EGU General Assembly 2010

DNA methylation dynamics in muscle development and disease

Directory of Open Access Journals (Sweden)

Elvira eCarrio

2015-03-01

Full Text Available DNA methylation is an essential epigenetic modification for mammalian development and is crucial for the establishment and maintenance of cellular identity. Traditionally, DNA methylation has been considered as a permanent repressive epigenetic mark. However, the application of genome-wide approaches has allowed the analysis of DNA methylation in different genomic contexts revealing a more dynamic regulation than originally thought, since active DNA methylation and demethylation occur during cellular differentiation and tissue specification. Satellite cells are the primary stem cells in adult skeletal muscle and are responsible for postnatal muscle growth, hypertrophy, and muscle regeneration. This review outlines the published data regarding DNA methylation changes along the skeletal muscle program, in both physiological and pathological conditions, to better understand the epigenetic mechanisms that control myogenesis

High-frequency remote monitoring of large lakes with MODIS 500 m imagery

Science.gov (United States)

McCullough, Ian M.; Loftin, Cynthia S.; Sader, Steven A.

2012-01-01

Satellite-based remote monitoring programs of regional lake water quality largely have relied on Landsat Thematic Mapper (TM) owing to its long image archive, moderate spatial resolution (30 m), and wide sensitivity in the visible portion of the electromagnetic spectrum, despite some notable limitations such as temporal resolution (i.e., 16 days), data pre-processing requirements to improve data quality, and aging satellites. Moderate-Resolution Imaging Spectroradiometer (MODIS) sensors on Aqua/Terra platforms compensate for these shortcomings, although at the expense of spatial resolution. We developed and evaluated a remote monitoring protocol for water clarity of large lakes using MODIS 500 m data and compared MODIS utility to Landsat-based methods. MODIS images captured during May–September 2001, 2004 and 2010 were analyzed with linear regression to identify the relationship between lake water clarity and satellite-measured surface reflectance. Correlations were strong (R² = 0.72–0.94) throughout the study period; however, they were the most consistent in August, reflecting seasonally unstable lake conditions and inter-annual differences in algal productivity during the other months. The utility of MODIS data in remote water quality estimation lies in intra-annual monitoring of lake water clarity in inaccessible, large lakes, whereas Landsat is more appropriate for inter-annual, regional trend analyses of lakes ≥ 8 ha. Model accuracy is improved when ancillary variables are included to reflect seasonal lake dynamics and weather patterns that influence lake clarity. The identification of landscape-scale drivers of regional water quality is a useful way to supplement satellite-based remote monitoring programs relying on spectral data alone.

Minimizing Gaps of Daily Ndvi Map with Geostationary Satellite Remote Sensing Data

Science.gov (United States)

Lee, S.; Ryu, Y.; Jiang, C.

2015-12-01

Satellite based remote sensing has been used to monitor plant phenology. Numerous studies have generally utilized normalized difference vegetation index (NDVI) to quantify phenological patterns and changes in regional to the global scales. Obtaining the NDVI values during summer in East Asian Monsoon regions is important because most plants grow vigorously in this season. However, satellite derived NDVI data are error prone to clouds during most of the period. Various methods have attempted to reduce the effect of cloud in temporal and spatial NDVI monitoring; the fundamental solution is to have a large data pool that includes multiple images in short period and supplements NDVI values in same period. Multiple images of geostationary satellite in a day can be a method to expand the pool. In this study, we suggest an approach that minimizes data gaps in NDVI of the day through geostationary satellite derived NDVI composition. We acquired data from Geostationary Ocean Color Imager (GOCI) which is a satellite that was launched to monitor ocean around the Korean peninsula, China, Japan and Russia. The satellite observes eight times per day (09:00 - 16:00, every hour) at 500 x 500 m resolution from 2011 to 2015. GOCI red- and near infrared radiance was converted into surface reflectance by using 6S Radiative Transfer Model (6S). We calculated NDVI tiles for each of observed eight tiles per day and made one day NDVI through maximum-value composite method. We evaluated the composite GOCI derived NDVI by comparing with daily MODIS-derived NDVI (composited from MOD09GA and MYD09GA), 16-day Landsat 8-derived NDVI, and in-situ light emitting diode (LED) NDVI measurements at a homogeneous deciduous forest and rice paddy sites. We found that GOCI-derived NDVI maps revealed little data gaps compared to MODIS and Landsat, and GOCI derived NDVI time series were smoother than MODIS derived NDVI time series in summer. GOCI-derived NDVI agreed well with in-situ observations of NDVI

A SERS-active sensor based on heterogeneous gold nanostar core-silver nanoparticle satellite assemblies for ultrasensitive detection of aflatoxinB1.

Science.gov (United States)

Li, Aike; Tang, Lijuan; Song, Dan; Song, Shanshan; Ma, Wei; Xu, Liguang; Kuang, Hua; Wu, Xiaoling; Liu, Liqiang; Chen, Xin; Xu, Chuanlai

2016-01-28

A surface-enhanced Raman scattering (SERS) sensor based on gold nanostar (Au NS) core-silver nanoparticle (Ag NP) satellites was fabricated for the first time to detect aflatoxinB1 (AFB1). We constructed the SERS sensor using AFB1 aptamer (DNA1)-modified Ag satellites and a complementary sequence (DNA2)-modified Au NS core. The Raman label (ATP) was modified on the surface of Ag satellites. The SERS signal was enhanced when the satellite NP was attached to the Au core NS. The AFB1 aptamer on the surface of Ag satellites would bind to the targets when AFB1 was present in the system, Ag satellites were then removed and the SERS signal decreased. This SERS sensor showed superior specificity for AFB1 and the linear detection range was from 1 to 1000 pg mL(-1) with the limit of detection (LOD) of 0.48 pg mL(-1). The excellent recovery experiment using peanut milk demonstrated that the sensor could be applied in food and environmental detection.

Updating the maize karyotype by chromosome DNA sizing

Science.gov (United States)

2018-01-01

The karyotype is a basic concept regarding the genome, fundamentally described by the number and morphological features of all chromosomes. Chromosome class, centromeric index, intra- and interchromosomal asymmetry index, and constriction localization are important in clinical, systematic and evolutionary approaches. In spite of the advances in karyotype characterization made over the last years, new data about the chromosomes can be generated from quantitative methods, such as image cytometry. Therefore, using Zea mays L., this study aimed to update the species’ karyotype by supplementing information on chromosome DNA sizing. After adjustment of the procedures, chromosome morphometry and class as well as knob localization enabled describing the Z. mays karyotype. In addition, applying image cytometry, DNA sizing was unprecedentedly measured for the arms and satellite of all chromosomes. This way, unambiguous identification of the chromosome pairs, and hence the assembly of 51 karyograms, were only possible after the DNA sizing of each chromosome, their arms and satellite portions. These accurate, quantitative and reproducible data also enabled determining the distribution and variation of DNA content in each chromosome. From this, a correlation between DNA amount and total chromosome length evidenced that the mean DNA content of chromosome 9 was higher than that of chromosome 8. The chromosomal DNA sizing updated the Z. mays karyotype, providing insights into its dynamic genome with regards to the organization of the ten chromosomes and their respective portions. Considering the results and the relevance of cytogenetics in the current scenario of comparative sequencing and genomics, chromosomal DNA sizing should be incorporated as an additional parameter for karyotype definition. Based on this study, it can be affirmed that cytogenetic approaches go beyond the simple morphological description of chromosomes. PMID:29293613

Localization of specific sequences and DNA single-strand breaks in individual UV-A-irradiated human lymphocytes by COMET FISH

Science.gov (United States)

Bock, Claudia; Rapp, Alexander; Dittmar, Heike; Monajembashi, Shamci; Greulich, Karl-Otto

1999-01-01

The COMET assay, a single cell electrophoresis technique which allows to separate electrophoretically fractionated DNA according to size has been combined with fluorescence in situ hybridization (FISH) which allows to localize specific genes or gene regions. This combination (COMET FISH) allows the detection of DNA single strand breaks in specific regions of the genome of human lymphocytes at the single cell level. Various types of DNA probes, e.g. centromere-, (alpha) - satellite-, telomere-, whole chromosome-, single copy- and region specific DNA probes have been used to investigate whether the UV-A induced DNA single strand breaks are distributed randomly all over the human genome or induced at specific sites ('hot spots'). In the investigated human peripheral blood lymphocytes all but one centromere reveal low sensitivity for UV-A irradiation (500 kJ/m2), while telomeres are randomly distributed over COMET heads and tails. The human chromosome 1 is fractionated by irradiation, but remains in the COMET head, indicating an only moderate degree of fractionation. Among three tested single copy probes, c- myc, p53 and p58, the p53 gene located on chromosome 17p13.1 and the p58 gene (1p36) appear to be located in UV-A stable regions of the human genome in 95% of 65 investigated lymphocytes. In contrast, the c-myc proto-oncogene (8q24) is found in the COMET tail in 90% of the 27 investigated lymphocytes and thus appears to be more sensitive to UV-A irradiation.

UVB DNA dosimeters analyzed by polymerase chain reactors

International Nuclear Information System (INIS)

Yoshida, Hiroko; Regan, J.D.; Florida Inst. of Tech., Melbourne, FL

1997-01-01

Purified bacteriophage λ DNA was dried on a UV-transparent polymer film and served as a UVB dosimeter for personal and ecological applications. Bacteriophage λ DNA was chosen because it is commercially available and inexpensive, and its entire sequence is known. Each dosimeter contained two sets of DNA sandwiched between UV-transparent polymer films, one exposed to solar radiation (experimental) and another protected from UV radiation by black paper (control). The DNA dosimeter was then analyzed by a polymerase chain reaction (PCR) that amplifies a 500 base pair specific region of λ DNA. Photoinduced damage in DNA blocks polymerase from synthesizing a new strand; therefore, the amount of amplified product in UV-exposed DNA was reduced from that found in control DNA. The dried λ DNA dosimeter is compact, robust, safe and transportable, stable over long storage times and provides the total UVB dose integrated over the exposure time. (author)

DNA repair phenotype and dietary antioxidant supplementation

DEFF Research Database (Denmark)

Guarnieri, Serena; Loft, Steffen; Riso, Patrizia

2008-01-01

Phytochemicals may protect cellular DNA by direct antioxidant effect or modulation of the DNA repair activity. We investigated the repair activity towards oxidised DNA in human mononuclear blood cells (MNBC) in two placebo-controlled antioxidant intervention studies as follows: (1) well-nourished......Phytochemicals may protect cellular DNA by direct antioxidant effect or modulation of the DNA repair activity. We investigated the repair activity towards oxidised DNA in human mononuclear blood cells (MNBC) in two placebo-controlled antioxidant intervention studies as follows: (1) well......-nourished subjects who ingested 600 g fruits and vegetables, or tablets containing the equivalent amount of vitamins and minerals, for 24 d; (2) poorly nourished male smokers who ingested 500 mg vitamin C/d as slow- or plain-release formulations together with 182 mg vitamin E/d for 4 weeks. The mean baseline levels...

Observation of cosmic-ray particles with artificial satellites in Japan

International Nuclear Information System (INIS)

Nagata, Katsuaki

1981-01-01

The present status are described on the cosmic-ray observation with artificial satellites in Japan. In 1978, an electrostatic analyzer was loaded on the satellite EXOS-A to measure low energy electrons. The spectra taken on April 27, 1978, showed that the electron flux decreased exponentially with the increasing electron energy. A space environment monitor (SEM) was loaded on a geostationary meteorological satellite (GMS) in 1977. The SEM consists of 5 Si detectors, with which particle identification can be made, and protons with the energy of 500 MeV and alpha particles with the energy of 370 MeV were observed. The time variation of particle flux was large in the low energy part and small in the high energy part. In 1984, the satellite EXOS-C will be launched. The purposes of this project are general observation of the middle atmosphere composition and the study of the anomaly of the ionosphere above the Brazilian Anomaly. Measurement of low energy particles will be done with an electrostatic analyzer, and that of high energy particles with a telescope with Si detectors. Other projects designed in Japan are OPEN-J and EXOS-D. (Kato, T.)

Phillips Laboratory small satellite initiatives

Science.gov (United States)

Lutey, Mark K.; Imler, Thomas A.; Davis, Robert J.

1993-09-01

The Phillips Laboratory Space Experiments Directorate in conjunction with the Air Force Space Test Program (AF STP), Defense Advanced Research and Projects Agency (DARPA) and Strategic Defense Initiative Organization (SDIO), are managing five small satellite program initiatives: Lightweight Exo-Atmospheric Projectile (LEAP) sponsored by SDIO, Miniature Sensor Technology Integration (MSTI) sponsored by SDIO, Technology for Autonomous Operational Survivability (TAOS) sponsored by Phillips Laboratory, TechSat sponsored by SDIO, and the Advanced Technology Standard Satellite Bus (ATSSB) sponsored by DARPA. Each of these spacecraft fulfills a unique set of program requirements. These program requirements range from a short-lived `one-of-a-kind' mission to the robust multi- mission role. Because of these diverging requirements, each program is driven to use a different design philosophy. But regardless of their design, there is the underlying fact that small satellites do not always equate to small missions. These spacecraft with their use of or ability to insert new technologies provide more capabilities and services for their respective payloads which allows the expansion of their mission role. These varying program efforts culminate in an ATSSB spacecraft bus approach that will support moderate size payloads, up to 500 pounds, in a large set of orbits while satisfying the `cheaper, faster, better' method of doing business. This technical paper provides an overview of each of the five spacecraft, focusing on the objectives, payoffs, technologies demonstrated, and program status.

Characterization of Botrytis cinerea isolates from chickpea: DNA ...

African Journals Online (AJOL)

Administrator

2010-11-15

Nov 15, 2010 ... 2Department of Plant Sciences, University of Hyderabad, Hyderabad 500 046, India. ... similarity of the isolates varied from 14-44%, and the isolates were separated ..... application to human mitochondrial DNA restriction sites.

C/NOFS Satellite Electric Field and Plasma Density Observations of Plasma Instabilities Below the Equatorial F-Peak -- Evidence for Approximately 500 km-Scale Spread-F "Precursor" Waves Driven by Zonal Shear Flow and km-Scale, Narrow-Banded Irregularities

Science.gov (United States)

Pfaff, R.; Freudenreich, H.; Klenzing, J.; Liebrecht, C.; Valladares, C.

2011-01-01

As solar activity has increased, the ionosphere F-peak has been elevated on numerous occasions above the C/NOFS satellite perigee of 400km. In particular, during the month of April, 2011, the satellite consistently journeyed below the F-peak whenever the orbit was in the region of the South Atlantic anomaly after sunset. During these passes, data from the electric field and plasma density probes on the satellite have revealed two types of instabilities which had not previously been observed in the C/NOFS data set (to our knowledge): The first is evidence for 400-500km-scale bottomside "undulations" that appear in the density and electric field data. In one case, these large scale waves are associated with a strong shear in the zonal E x B flow, as evidenced by variations in the meridional (outward) electric fields observed above and below the F-peak. These undulations are devoid of smaller scale structures in the early evening, yet appear at later local times along the same orbit associated with fully-developed spread-F with smaller scale structures. This suggests that they may be precursor waves for spread-F, driven by a collisional shear instability, following ideas advanced previously by researchers using data from the Jicamarca radar. A second new result (for C/NOFS) is the appearance of km-scale irregularities that are a common feature in the electric field and plasma density data that also appear when the satellite is below the F -peak at night. The vector electric field instrument on C/NOFS clearly shows that the electric field component of these waves is strongest in the zonal direction. These waves are strongly correlated with simultaneous observations of plasma density oscillations and appear both with, and without, evidence of larger-scale spread-F depletions. These km-scale, quasi-coherent waves strongly resemble the bottomside, sinusoidal irregularities reported in the Atmosphere Explorer satellite data set by Valladares et al. [JGR, 88, 8025, 1983

Impact of heating on sensory properties of French Protected Designation of Origin (PDO) blue cheeses. Relationships with physicochemical parameters.

Science.gov (United States)

Bord, Cécile; Guerinon, Delphine; Lebecque, Annick

2016-07-01

The aim of this study was to measure the impact of heating on the sensory properties of blue-veined cheeses in order to characterise their sensory properties and to identify their specific sensory typology associated with physicochemical parameters. Sensory profiles were performed on a selection of Protected Designation of Origin (PDO) cheeses representing the four blue-veined cheese categories produced in the Massif Central (Fourme d'Ambert, Fourme de Montbrison, Bleu d'Auvergne and Bleu des Causses). At the same time, physicochemical parameters were measured in these cheeses. The relationship between these two sets of data was investigated. Four types of blue-veined cheeses displayed significantly different behaviour after heating and it is possible to discriminate these cheese categories through specific sensory attributes. Fourme d'Ambert and Bleu d'Auvergne exhibited useful culinary properties: they presented good meltability, stretchability and a weak oiling-off. However, basic tastes (salty, bitter and sour) are also sensory attributes which can distinguish heated blue cheeses. The relationship between the sensory and physicochemical data indicated a correlation suggesting that some of these sensory properties may be explained by certain physicochemical parameters of heated cheeses. © The Author(s) 2015.

Comparative Analysis of Repetitive DNA between the Main Vectors of Chagas Disease: Triatoma infestans and Rhodnius prolixus.

Science.gov (United States)

Pita, Sebastián; Mora, Pablo; Vela, Jesús; Palomeque, Teresa; Sánchez, Antonio; Panzera, Francisco; Lorite, Pedro

2018-04-24

Chagas disease or American trypanosomiasis affects six to seven million people worldwide, mostly in Latin America. This disease is transmitted by hematophagous insects known as "kissing bugs" (Hemiptera, Triatominae), with Triatoma infestans and Rhodnius prolixus being the two most important vector species. Despite the fact that both species present the same diploid chromosome number (2 n = 22), they have remarkable differences in their total DNA content, chromosome structure and genome organization. Variations in the DNA genome size are expected to be due to differences in the amount of repetitive DNA sequences. The T. infestans genome-wide analysis revealed the existence of 42 satellite DNA families. BLAST searches of these sequences against the R. prolixus genome assembly revealed that only four of these satellite DNA families are shared between both species, suggesting a great differentiation between the Triatoma and Rhodnius genomes. Fluorescence in situ hybridization (FISH) location of these repetitive DNAs in both species showed that they are dispersed on the euchromatic regions of all autosomes and the X chromosome. Regarding the Y chromosome, these common satellite DNAs are absent in T. infestans but they are present in the R. prolixus Y chromosome. These results support a different origin and/or evolution in the Y chromosome of both species.

The organization and evolution of the Responder satellite in species of the Drosophila melanogaster group: dynamic evolution of a target of meiotic drive.

Science.gov (United States)

Larracuente, Amanda M

2014-11-25

Satellite DNA can make up a substantial fraction of eukaryotic genomes and has roles in genome structure and chromosome segregation. The rapid evolution of satellite DNA can contribute to genomic instability and genetic incompatibilities between species. Despite its ubiquity and its contribution to genome evolution, we currently know little about the dynamics of satellite DNA evolution. The Responder (Rsp) satellite DNA family is found in the pericentric heterochromatin of chromosome 2 of Drosophila melanogaster. Rsp is well-known for being the target of Segregation Distorter (SD)- an autosomal meiotic drive system in D. melanogaster. I present an evolutionary genetic analysis of the Rsp family of repeats in D. melanogaster and its closely-related species in the melanogaster group (D. simulans, D. sechellia, D. mauritiana, D. erecta, and D. yakuba) using a combination of available BAC sequences, whole genome shotgun Sanger reads, Illumina short read deep sequencing, and fluorescence in situ hybridization. I show that Rsp repeats have euchromatic locations throughout the D. melanogaster genome, that Rsp arrays show evidence for concerted evolution, and that Rsp repeats exist outside of D. melanogaster, in the melanogaster group. The repeats in these species are considerably diverged at the sequence level compared to D. melanogaster, and have a strikingly different genomic distribution, even between closely-related sister taxa. The genomic organization of the Rsp repeat in the D. melanogaster genome is complex-it exists of large blocks of tandem repeats in the heterochromatin and small blocks of tandem repeats in the euchromatin. My discovery of heterochromatic Rsp-like sequences outside of D. melanogaster suggests that SD evolved after its target satellite and that the evolution of the Rsp satellite family is highly dynamic over a short evolutionary time scale (<240,000 years).

28 CFR 54.500 - Employment.

Science.gov (United States)

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Employment. 54.500 Section 54.500... in Employment in Education Programs or Activities Prohibited § 54.500 Employment. (a) General. (1) No... subjected to discrimination in employment, or recruitment, consideration, or selection therefor, whether...

Protective effect of a polyvalent influenza DNA vaccine in pigs

DEFF Research Database (Denmark)

Karlsson, Ingrid; Borggren, Marie; Rosenstierne, Maiken Worsøe

2018-01-01

Background Influenza A virus in swine herds represents a major problem for the swine industry and poses a constant threat for the emergence of novel pandemic viruses and the development of more effective influenza vaccines for pigs is desired. By optimizing the vector backbone and using a needle...... needle-free delivery to the skin, we immunized pigs with two different doses (500 μg and 800 μg) of an influenza DNA vaccine based on six genes of pandemic origin, including internally expressed matrix and nucleoprotein and externally expressed hemagglutinin and neuraminidase as previously demonstrated....... Two weeks following immunization, the pigs were challenged with the 2009 pandemic H1N1 virus. Results When challenged with 2009 pandemic H1N1, 0/5 vaccinated pigs (800 μg DNA) became infected whereas 5/5 unvaccinated control pigs were infected. The pigs vaccinated with the low dose (500 μg DNA) were...

Radiation-induced changes in DNA methylation of repetitive elements in the mouse heart

Energy Technology Data Exchange (ETDEWEB)

Koturbash, Igor, E-mail: ikoturbash@uams.edu [Department of Environmental and Occupational Health, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Miousse, Isabelle R. [Department of Environmental and Occupational Health, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Sridharan, Vijayalakshmi [Division of Radiation Health, Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Nzabarushimana, Etienne; Skinner, Charles M. [Department of Environmental and Occupational Health, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Melnyk, Stepan B.; Pavliv, Oleksandra [Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Hauer-Jensen, Martin [Division of Radiation Health, Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States); Surgical Service, Central Arkansas Veterans Healthcare System, Little Rock, AR 72205 (United States); Nelson, Gregory A. [Departments of Basic Sciences and Radiation Medicine, Loma Linda University, Loma Linda, CA 92354 (United States); Boerma, Marjan [Division of Radiation Health, Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, Little Rock, AR 72205 (United States)

2016-05-15

Highlights: • Radiation-induced dynamic changes in cardiac DNA methylation were detected. • Early LINE-1 hypomethylation was followed by hypermethylation at a later time-point. • Radiation affected one-carbon metabolism in the heart tissue. • Irradiation resulted in accumulation of satellite DNA mRNA transcripts. - Abstract: DNA methylation is a key epigenetic mechanism, needed for proper control over the expression of genetic information and silencing of repetitive elements. Exposure to ionizing radiation, aside from its strong genotoxic potential, may also affect the methylation of DNA, within the repetitive elements, in particular. In this study, we exposed C57BL/6J male mice to low absorbed mean doses of two types of space radiation—proton (0.1 Gy, 150 MeV, dose rate 0.53 ± 0.08 Gy/min), and heavy iron ions ({sup 56}Fe) (0.5 Gy, 600 MeV/n, dose rate 0.38 ± 0.06 Gy/min). Radiation-induced changes in cardiac DNA methylation associated with repetitive elements were detected. Specifically, modest hypomethylation of retrotransposon LINE-1 was observed at day 7 after irradiation with either protons or {sup 56}Fe. This was followed by LINE-1, and other retrotransposons, ERV2 and SINE B1, as well as major satellite DNA hypermethylation at day 90 after irradiation with {sup 56}Fe. These changes in DNA methylation were accompanied by alterations in the expression of DNA methylation machinery and affected the one-carbon metabolism pathway. Furthermore, loss of transposable elements expression was detected in the cardiac tissue at the 90-day time-point, paralleled by substantial accumulation of mRNA transcripts, associated with major satellites. Given that the one-carbon metabolism pathway can be modulated by dietary modifications, these findings suggest a potential strategy for the mitigation and, possibly, prevention of the negative effects exerted by ionizing radiation on the cardiovascular system. Additionally, we show that the methylation status and

23 CFR 500.109 - CMS.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false CMS. 500.109 Section 500.109 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRANSPORTATION INFRASTRUCTURE MANAGEMENT MANAGEMENT AND MONITORING SYSTEMS Management Systems § 500.109 CMS. (a) For purposes of this part, congestion means the level at...

29 CFR 500.0 - Introduction.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Introduction. 500.0 Section 500.0 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION General Provisions § 500.0 Introduction. (a) The Migrant and Seasonal...

43 CFR 41.500 - Employment.

Science.gov (United States)

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false Employment. 41.500 Section 41.500 Public... in Employment in Education Programs or Activities Prohibited § 41.500 Employment. (a) General. (1) No... subjected to discrimination in employment, or recruitment, consideration, or selection therefor, whether...

6 CFR 17.500 - Employment.

Science.gov (United States)

2010-01-01

... 6 Domestic Security 1 2010-01-01 2010-01-01 false Employment. 17.500 Section 17.500 Domestic... in Employment in Education Programs or Activities Prohibited § 17.500 Employment. (a) General. (1) No... subjected to discrimination in employment, or recruitment, consideration, or selection therefore, whether...

40 CFR 5.500 - Employment.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Employment. 5.500 Section 5.500... in Employment in Education Programs or Activities Prohibited § 5.500 Employment. (a) General. (1) No... subjected to discrimination in employment, or recruitment, consideration, or selection therefor, whether...

14 CFR 1253.500 - Employment.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Employment. 1253.500 Section 1253.500... in Employment in Education Programs or Activities Prohibited § 1253.500 Employment. (a) General. (1..., or be subjected to discrimination in employment, or recruitment, consideration, or selection therefor...

Evolutionary dynamics and sites of illegitimate recombination revealed in the interspersion and sequence junctions of two nonhomologous satellite DNAs in cactophilic Drosophila species.

Science.gov (United States)

Kuhn, G C S; Teo, C H; Schwarzacher, T; Heslop-Harrison, J S

2009-05-01

Satellite DNA (satDNA) is a major component of genomes but relatively little is known about the fine-scale organization of unrelated satDNAs residing at the same chromosome location, and the sequence structure and dynamics of satDNA junctions. We studied the organization and sequence junctions of two nonhomologous satDNAs, pBuM and DBC-150, in three species from the neotropical Drosophila buzzatii cluster (repleta group). In situ hybridization to microchromosomes, interphase nuclei and extended DNA fibers showed frequent interspersion of the two satellites in D. gouveai, D. antonietae and, to a lesser extent, D. seriema. We isolated by PCR six pBuM x DBC-150 junctions: four are exclusive to D. gouveai and two are exclusive to D. antonietae. The six junction breakpoints occur at different positions within monomers, suggesting independent origin. Four junctions showed abrupt transitions between the two satellites, whereas two junctions showed a distinct 10 bp tandem duplication before the junction. Unlike pBuM, DBC-150 junction repeats are more variable than randomly cloned monomers and showed diagnostic features in common to a 3-monomer higher-order repeat seen in the sister species D. serido. The high levels of interspersion between pBuM and DBC-150 repeats suggest extensive rearrangements between the two satellites, maybe favored by specific features of the microchromosomes. Our interpretation is that the junctions evolved by multiples events of illegitimate recombination between nonhomologous satDNA repeats, with subsequent rounds of unequal crossing-over expanding the copy number of some of the junctions.

The X-linked 1.688 Satellite in Drosophila melanogaster Promotes Specific Targeting by Painting of Fourth.

Science.gov (United States)

Kim, Maria; Ekhteraei-Tousi, Samaneh; Lewerentz, Jacob; Larsson, Jan

2018-02-01

Repetitive DNA, represented by transposons and satellite DNA, constitutes a large portion of eukaryotic genomes, being the major component of constitutive heterochromatin. There is a growing body of evidence that it regulates several nuclear functions including chromatin state and the proper functioning of centromeres and telomeres. The 1.688 satellite is one of the most abundant repetitive sequences in Drosophila melanogaster , with the longest array being located in the pericentromeric region of the X-chromosome. Short arrays of 1.688 repeats are widespread within the euchromatic part of the X-chromosome, and these arrays were recently suggested to assist in recognition of the X-chromosome by the dosage compensation male-specific lethal complex. We discovered that a short array of 1.688 satellite repeats is essential for recruitment of the protein POF to a previously described site on the X-chromosome ( PoX2 ) and to various transgenic constructs. On an isolated target, i.e. , an autosomic transgene consisting of a gene upstream of 1.688 satellite repeats, POF is recruited to the transgene in both males and females. The sequence of the satellite, as well as its length and position within the recruitment element, are the major determinants of targeting. Moreover, the 1.688 array promotes POF targeting to the roX1 -proximal PoX1 site in trans Finally, binding of POF to the 1.688-related satellite-enriched sequences is conserved in evolution. We hypothesize that the 1.688 satellite functioned in an ancient dosage compensation system involving POF targeting to the X-chromosome. Copyright © 2018 by the Genetics Society of America.

21 CFR 556.500 - Oxytetracycline.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Oxytetracycline. 556.500 Section 556.500 Food and... Residues of New Animal Drugs § 556.500 Oxytetracycline. (a) Acceptable daily intake (ADI). The ADI for total tetracycline residues (chlortetracycline, oxytetracycline, and tetracycline) is 25 micrograms per...

29 CFR 500.155 - Authority.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Authority. 500.155 Section 500.155 Labor Regulations... AGRICULTURAL WORKER PROTECTION Enforcement Agreements with Federal and State Agencies § 500.155 Authority...) to Federal and State agencies such authority (other than rulemaking) as he determines may be useful...

1 CFR 500.140 - Employment.

Science.gov (United States)

2010-01-01

... 1 General Provisions 1 2010-01-01 2010-01-01 false Employment. 500.140 Section 500.140 General... ACTIVITIES CONDUCTED BY THE NATIONAL COMMISSION FOR EMPLOYMENT POLICY § 500.140 Employment. No qualified handicapped person shall, on the basis of handicap, be subjected to discrimination in employment under any...

22 CFR 229.500 - Employment.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Employment. 229.500 Section 229.500 Foreign... OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 229.500 Employment. (a) General. (1) No person shall...

22 CFR 146.500 - Employment.

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2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Employment. 146.500 Section 146.500 Foreign... ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.500 Employment. (a) General. (1) No person shall, on the...

16 CFR 500.17 - Fractions.

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2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Fractions. 500.17 Section 500.17 Commercial... LABELING ACT § 500.17 Fractions. (a) SI metric declarations of net quantity of contents of any consumer commodity may contain only decimal fractions. Other declarations of net quantity of contents may contain...

7 CFR 500.6 - Gambling.

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2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Gambling. 500.6 Section 500.6 Agriculture Regulations... NATIONAL ARBORETUM Conduct on U.S. National Arboreturm Property § 500.6 Gambling. Participating in games for money or other personal property, or the operation of gambling devices, the conduct of a lottery...

9 CFR 93.500 - Definitions.

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2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Definitions. 93.500 Section 93.500... CONVEYANCE AND SHIPPING CONTAINERS Swine § 93.500 Definitions. Wherever in this subpart the following terms..., Spain, Sweden, and the United Kingdom (England, Scotland, Wales, the Isle of Man, and Northern Ireland...

21 CFR 500.82 - Definitions.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Definitions. 500.82 Section 500.82 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS GENERAL Regulation of Carcinogenic Compounds Used in Food-Producing Animals § 500...

29 CFR 500.162 - Reports.

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2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Reports. 500.162 Section 500.162 Labor Regulations Relating... AGRICULTURAL WORKER PROTECTION Enforcement Agreements with Federal and State Agencies § 500.162 Reports. The Secretary shall require such reports as he deems necessary of activities conducted pursuant to State plans...

24 CFR 180.500 - Discovery.

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2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Discovery. 180.500 Section 180.500... OPPORTUNITY CONSOLIDATED HUD HEARING PROCEDURES FOR CIVIL RIGHTS MATTERS Discovery § 180.500 Discovery. (a) In general. This subpart governs discovery in aid of administrative proceedings under this part. Discovery in...

13 CFR 500.105 - Staff.

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2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Staff. 500.105 Section 500.105... LOAN PROGRAM Board Procedures § 500.105 Staff. (a) Executive Director. The Executive Director of the... direction with respect to the administration of the Board's actions, directs the activities of the staff...

10 CFR 1042.500 - Employment.

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2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Employment. 1042.500 Section 1042.500 Energy DEPARTMENT OF... RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1042.500 Employment. (a) General. (1) No person shall, on the basis of...

45 CFR 618.500 - Employment.

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2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Employment. 618.500 Section 618.500 Public Welfare... the Basis of Sex in Employment in Education Programs or Activities Prohibited § 618.500 Employment. (a... benefits of, or be subjected to discrimination in employment, or recruitment, consideration, or selection...

24 CFR 214.500 - Audit.

Science.gov (United States)

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Audit. 214.500 Section 214.500... PROGRAM Other Federal Requirements § 214.500 Audit. Housing counseling grant recipients and subrecipients shall be subject to the audit requirements contained in 24 CFR parts 84 and 85. HUD must be provided a...

10 CFR 5.500 - Employment.

Science.gov (United States)

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Employment. 5.500 Section 5.500 Energy NUCLEAR REGULATORY... FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 5.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from...

32 CFR 196.500 - Employment.

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2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Employment. 196.500 Section 196.500 National... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 196.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

45 CFR 2555.500 - Employment.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 4 2010-10-01 2010-10-01 false Employment. 2555.500 Section 2555.500 Public... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 2555.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

23 CFR 500.104 - State option.

Science.gov (United States)

2010-04-01

... 23 Highways 1 2010-04-01 2010-04-01 false State option. 500.104 Section 500.104 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRANSPORTATION INFRASTRUCTURE MANAGEMENT MANAGEMENT AND MONITORING SYSTEMS Management Systems § 500.104 State option. Except as specified in § 500.105 (a...

Chromosome-specific DNA Repeat Probes

Energy Technology Data Exchange (ETDEWEB)

Baumgartner, Adolf; Weier, Jingly Fung; Weier, Heinz-Ulrich G.

2006-03-16

In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific nucleic acid probes. We describe two approaches for an expeditious preparation of chromosome-specific DNAs and the subsequent probe labeling with reporter molecules of choice. The described techniques allow the preparation of highly specific DNA repeat probes suitable for enumeration of chromosomes in interphase cell nuclei or tissue sections. In addition, there is no need for chromosome enrichment by flow cytometry and sorting or molecular cloning. Our PCR-based method uses either bacterial artificial chromosomes or human genomic DNA as templates with {alpha}-satellite-specific primers. Here we demonstrate the production of fluorochrome-labeled DNA repeat probes specific for human chromosomes 17 and 18 in just a few days without the need for highly specialized equipment and without the limitation to only a few fluorochrome labels.

24 CFR 3.500 - Employment.

Science.gov (United States)

2010-04-01

... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Employment. 3.500 Section 3.500... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 3.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

Observation of ESR spin flip satellite lines of trapped hydrogen atoms in solid H2 at 4.2 K

International Nuclear Information System (INIS)

Miyazaki, Tetsuo; Iwata, Nobuchika; Fueki, Kenji; Hase, Hirotomo

1990-01-01

ESR spectra of H atoms, produced in γ-irradiated solid H 2 , were studied at 4.2 K. Two main lines of the ESR spectra of H atoms that are separated by about 500 G accompanied two weak satellite lines. Both satellite lines and main lines decrease with the same decay rate. In the D 2 -H 2 mixtures, the satellite-line intensity depends upon the number of matrix protons. The spacing of the satellites from the main lines is equal to that of the NMR proton resonance frequency. It was concluded that the satellite lines were not ascribable to paired atoms but to spin flip lines due to an interaction of H atoms with matrix protons. The analysis of the spin flip lines and the main lines suggests that H atoms in solid H 2 are trapped in the substitutional site

Organization and evolution of primate centromeric DNA from whole-genome shotgun sequence data.

Directory of Open Access Journals (Sweden)

Can Alkan

2007-09-01

Full Text Available The major DNA constituent of primate centromeres is alpha satellite DNA. As much as 2%-5% of sequence generated as part of primate genome sequencing projects consists of this material, which is fragmented or not assembled as part of published genome sequences due to its highly repetitive nature. Here, we develop computational methods to rapidly recover and categorize alpha-satellite sequences from previously uncharacterized whole-genome shotgun sequence data. We present an algorithm to computationally predict potential higher-order array structure based on paired-end sequence data and then experimentally validate its organization and distribution by experimental analyses. Using whole-genome shotgun data from the human, chimpanzee, and macaque genomes, we examine the phylogenetic relationship of these sequences and provide further support for a model for their evolution and mutation over the last 25 million years. Our results confirm fundamental differences in the dispersal and evolution of centromeric satellites in the Old World monkey and ape lineages of evolution.

Organization and evolution of primate centromeric DNA from whole-genome shotgun sequence data.

Science.gov (United States)

Alkan, Can; Ventura, Mario; Archidiacono, Nicoletta; Rocchi, Mariano; Sahinalp, S Cenk; Eichler, Evan E

2007-09-01

The major DNA constituent of primate centromeres is alpha satellite DNA. As much as 2%-5% of sequence generated as part of primate genome sequencing projects consists of this material, which is fragmented or not assembled as part of published genome sequences due to its highly repetitive nature. Here, we develop computational methods to rapidly recover and categorize alpha-satellite sequences from previously uncharacterized whole-genome shotgun sequence data. We present an algorithm to computationally predict potential higher-order array structure based on paired-end sequence data and then experimentally validate its organization and distribution by experimental analyses. Using whole-genome shotgun data from the human, chimpanzee, and macaque genomes, we examine the phylogenetic relationship of these sequences and provide further support for a model for their evolution and mutation over the last 25 million years. Our results confirm fundamental differences in the dispersal and evolution of centromeric satellites in the Old World monkey and ape lineages of evolution.

Uncovering the evolutionary history of neo-XY sex chromosomes in the grasshopper Ronderosia bergii (Orthoptera, Melanoplinae) through satellite DNA analysis.

Science.gov (United States)

Palacios-Gimenez, Octavio M; Milani, Diogo; Lemos, Bernardo; Castillo, Elio R; Martí, Dardo A; Ramos, Erica; Martins, Cesar; Cabral-de-Mello, Diogo C

2018-01-08

Neo-sex chromosome systems arose independently multiple times in evolution, presenting the remarkable characteristic of repetitive DNAs accumulation. Among grasshoppers, occurrence of neo-XY was repeatedly noticed in Melanoplinae. Here we analyzed the most abundant tandem repeats of R. bergii (2n = 22, neo-XY♂) using deep Illumina sequencing and graph-based clustering in order to address the neo-sex chromosomes evolution. The analyses revealed ten families of satDNAs comprising about ~1% of the male genome, which occupied mainly C-positive regions of autosomes. Regarding the sex chromosomes, satDNAs were recorded within centromeric or interstitial regions of the neo-X chromosome and four satDNAs occurred in the neo-Y, two of them being exclusive (Rber248 and Rber299). Using a combination of probes we uncovered five well-defined cytological variants for neo-Y, originated by multiple paracentric inversions and satDNA amplification, besides fragmented neo-Y. These neo-Y variants were distinct in frequency between embryos and adult males. The genomic data together with cytogenetic mapping enabled us to better understand the neo-sex chromosome dynamics in grasshoppers, reinforcing differentiation of neo-X and neo-Y and revealing the occurrence of multiple additional rearrangements involved in the neo-Y evolution of R. bergii. We discussed the possible causes that led to differences in frequency for the neo-Y variants between embryos and adults. Finally we hypothesize about the role of DNA satellites in R. bergii as well as putative historical events involved in the evolution of the R. bergii neo-XY.

Comparison of Hyperspectral and Multispectral Satellites for Discriminating Land Cover in Northern California

Science.gov (United States)

Clark, M. L.; Kilham, N. E.

2015-12-01

Land-cover maps are important science products needed for natural resource and ecosystem service management, biodiversity conservation planning, and assessing human-induced and natural drivers of land change. Most land-cover maps at regional to global scales are produced with remote sensing techniques applied to multispectral satellite imagery with 30-500 m pixel sizes (e.g., Landsat, MODIS). Hyperspectral, or imaging spectrometer, imagery measuring the visible to shortwave infrared regions (VSWIR) of the spectrum have shown impressive capacity to map plant species and coarser land-cover associations, yet techniques have not been widely tested at regional and greater spatial scales. The Hyperspectral Infrared Imager (HyspIRI) mission is a VSWIR hyperspectral and thermal satellite being considered for development by NASA. The goal of this study was to assess multi-temporal, HyspIRI-like satellite imagery for improved land cover mapping relative to multispectral satellites. We mapped FAO Land Cover Classification System (LCCS) classes over 22,500 km2 in the San Francisco Bay Area, California using 30-m HyspIRI, Landsat 8 and Sentinel-2 imagery simulated from data acquired by NASA's AVIRIS airborne sensor. Random Forests (RF) and Multiple-Endmember Spectral Mixture Analysis (MESMA) classifiers were applied to the simulated images and accuracies were compared to those from real Landsat 8 images. The RF classifier was superior to MESMA, and multi-temporal data yielded higher accuracy than summer-only data. With RF, hyperspectral data had overall accuracy of 72.2% and 85.1% with full 20-class and reduced 12-class schemes, respectively. Multispectral imagery had lower accuracy. For example, simulated and real Landsat data had 7.5% and 4.6% lower accuracy than HyspIRI data with 12 classes, respectively. In summary, our results indicate increased mapping accuracy using HyspIRI multi-temporal imagery, particularly in discriminating different natural vegetation types, such as

Efficient replication of the in vitro transcripts from cloned cDNA of tomato black ring virus satellite RNA requires the 48K satellite RNA-encoded protein.

Science.gov (United States)

Hemmer, O; Oncino, C; Fritsch, C

1993-06-01

Tomato black ring virus isolate L supports the multiplication of a large satellite RNA of 1376 nt which has no common features with the two genomic RNAs except for the terminal motif 5' VPg UUGAAAA and a 3' poly(A) tail. The TBRV sat-RNA contains an ORF for a protein of 48K which is translated both in vitro and in vivo. To determine the function of the 48K protein we have studied the effect of different mutations introduced in the ORF of the cDNA clone on the capacity of transcripts to multiply in Chenopodium quinoa plants or protoplasts when inoculated along with the genomic RNAs. Transcripts in which nucleotides have been substituted within the 5' proximal region of the ORF multiplied poorly even when the modification conserved the 48K protein sequence, suggesting that this portion of the ORF contains cis-acting RNA sequences. Transcripts with alterations in the internal region of the ORF retained their multiplication capacity provided the mutation did not destroy the ORF or modify the length of the protein expressed. The absence of multiplication in plants of transcripts unable to express the 48K protein and their inability to replicate in protoplasts suggest strongly that the sat-RNA translation product itself is implicated in the replication of sat-RNA.

Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.

Directory of Open Access Journals (Sweden)

Kiran Zahid

2015-01-01

Full Text Available Satellite RNAs (satRNAs are small noncoding subviral RNA pathogens in plants that depend on helper viruses for replication and spread. Despite many decades of research, the origin of satRNAs remains unknown. In this study we show that a β-glucuronidase (GUS transgene fused with a Cucumber mosaic virus (CMV Y satellite RNA (Y-Sat sequence (35S-GUS:Sat was transcriptionally repressed in N. tabacum in comparison to a 35S-GUS transgene that did not contain the Y-Sat sequence. This repression was not due to DNA methylation at the 35S promoter, but was associated with specific DNA methylation at the Y-Sat sequence. Both northern blot hybridization and small RNA deep sequencing detected 24-nt siRNAs in wild-type Nicotiana plants with sequence homology to Y-Sat, suggesting that the N. tabacum genome contains Y-Sat-like sequences that give rise to 24-nt sRNAs capable of guiding RNA-directed DNA methylation (RdDM to the Y-Sat sequence in the 35S-GUS:Sat transgene. Consistent with this, Southern blot hybridization detected multiple DNA bands in Nicotiana plants that had sequence homology to Y-Sat, suggesting that Y-Sat-like sequences exist in the Nicotiana genome as repetitive DNA, a DNA feature associated with 24-nt sRNAs. Our results point to a host genome origin for CMV satRNAs, and suggest novel approach of using small RNA sequences for finding the origin of other satRNAs.

A rapid and efficient DNA extraction protocol from fresh and frozen human blood samples.

Science.gov (United States)

Guha, Pokhraj; Das, Avishek; Dutta, Somit; Chaudhuri, Tapas Kumar

2018-01-01

Different methods available for extraction of human genomic DNA suffer from one or more drawbacks including low yield, compromised quality, cost, time consumption, use of toxic organic solvents, and many more. Herein, we aimed to develop a method to extract DNA from 500 μL of fresh or frozen human blood. Five hundred microliters of fresh and frozen human blood samples were used for standardization of the extraction procedure. Absorbance at 260 and 280 nm, respectively, (A 260 /A 280 ) were estimated to check the quality and quantity of the extracted DNA sample. Qualitative assessment of the extracted DNA was checked by Polymerase Chain reaction and double digestion of the DNA sample. Our protocol resulted in average yield of 22±2.97 μg and 20.5±3.97 μg from 500 μL of fresh and frozen blood, respectively, which were comparable to many reference protocols and kits. Besides yielding bulk amount of DNA, our protocol is rapid, economical, and avoids toxic organic solvents such as Phenol. Due to unaffected quality, the DNA is suitable for downstream applications. The protocol may also be useful for pursuing basic molecular researches in laboratories having limited funds. © 2017 Wiley Periodicals, Inc.

Satellite Tracking of Humpback Whales in West Greenland

DEFF Research Database (Denmark)

Dietz, R.; Teilmann, J.; Heide-Jørgensen, M.-P.

In June 2000, 6 humpback whales (Megaptere novaeangliae) were tagged with satellite transmitters off West Greenland. Contact remained for up to 42 days. The tagging revealed that within the month of June, humpback whales off West Greenland moved between Fiskenæs Banke, Fyllas Banke, Tovqussaq Banke......, Sukkertop Banke and Lille Hellefiske Banke. The whales showed a preference for the continental slopes with depths less than 200 m, however, few dives were recorded down to 500 m. The whales had a preference for dives lasting 7-8 min. (15%) and no dives lasted longer than 15 min....

29 CFR 500.9 - Discrimination prohibited.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Discrimination prohibited. 500.9 Section 500.9 Labor... SEASONAL AGRICULTURAL WORKER PROTECTION General Provisions § 500.9 Discrimination prohibited. (a) It is a... Secretary alleging such discrimination. ...

W-enriched satellite sequence in the Indian meal moth, Plodia interpunctella (Lepidoptera, Pyralidae).

Science.gov (United States)

Dalíková, Martina; Zrzavá, Magda; Kubíčková, Svatava; Marec, František

2017-10-01

The W chromosome of most lepidopteran species represents the largest heterochromatin entity in the female genome. Although satellite DNA is a typical component of constitutive heterochromatin, there are only a few known satellite DNAs (satDNAs) located on the W chromosome in moths and butterflies. In this study, we isolated and characterized new satDNA (PiSAT1) from microdissected W chromosomes of the Indian meal moth, Plodia interpunctella. Even though the PiSAT1 is mainly localized near the female-specific segment of the W chromosome, short arrays of this satDNA also occur on autosomes and/or the Z chromosome. Probably due to the predominant location in the non-recombining part of the genome, PiSAT1 exhibits a relatively large nucleotide variability in its monomers. However, at least a part of all predicted functional motifs is located in conserved regions. Moreover, we detected polyadenylated transcripts of PiSAT1 in all developmental stages and in both sexes (female and male larvae, pupae and adults). Our results suggest a potential structural and functional role of PiSAT1 in the P. interpunctella genome, which is consistent with accumulating evidence for the important role of satDNAs in eukaryotic genomes.

Mapping Russian forest biomass with data from satellites and forest inventories

International Nuclear Information System (INIS)

Houghton, R A; Butman, D; Bunn, A G; Krankina, O N; Schlesinger, P; Stone, T A

2007-01-01

The forests of Russia cover a larger area and hold more carbon than the forests of any other nation and thus have the potential for a major role in global warming. Despite a systematic inventory of these forests, however, estimates of total carbon stocks vary, and spatial variations in the stocks within large aggregated units of land are unknown, thus hampering measurement of sources and sinks of carbon. We mapped the distribution of living forest biomass for the year 2000 by developing a relationship between ground measurements of wood volume at 12 sites throughout the Russian Federation and data from the MODIS satellite bidirectional reflectance distribution function (BRDF) product (MOD43B4). Based on the results of regression-tree analyses, we used the MOD43B4 product to assign biomass values to individual 500 m x 500 m cells in areas identified as forest by two satellite-based maps of land cover. According to the analysis, the total living biomass varied between 46 and 67 Pg, largely because of different estimates of forest area. Although optical data are limited in distinguishing differences in biomass in closed canopy forests, the estimates of total living biomass obtained here varied more in response to different definitions of forest than to saturation of the optical sensing of biomass

Mapping Russian forest biomass with data from satellites and forest inventories

Energy Technology Data Exchange (ETDEWEB)

Houghton, R A [Woods Hole Research Center, 149 Woods Hole Road, Falmouth, MA 02540 (United States); Butman, D [Yale School of Forestry and Environmental Science, Yale University, New Haven, CT 06511 (United States); Bunn, A G [Department of Environmental Sciences, Huxley College of the Environment, Western Washington University, 516 High Street, Bellingham, WA 98225-9181 (United States); Krankina, O N [Department of Forest Science, Oregon State University, 202 Richardson Hall, Corvallis, OR 97331-5752 (United States); Schlesinger, P [Woods Hole Research Center, 149 Woods Hole Road, Falmouth, MA 02540 (United States); Stone, T A [Woods Hole Research Center, 149 Woods Hole Road, Falmouth, MA 02540 (United States)

2007-10-15

The forests of Russia cover a larger area and hold more carbon than the forests of any other nation and thus have the potential for a major role in global warming. Despite a systematic inventory of these forests, however, estimates of total carbon stocks vary, and spatial variations in the stocks within large aggregated units of land are unknown, thus hampering measurement of sources and sinks of carbon. We mapped the distribution of living forest biomass for the year 2000 by developing a relationship between ground measurements of wood volume at 12 sites throughout the Russian Federation and data from the MODIS satellite bidirectional reflectance distribution function (BRDF) product (MOD43B4). Based on the results of regression-tree analyses, we used the MOD43B4 product to assign biomass values to individual 500 m x 500 m cells in areas identified as forest by two satellite-based maps of land cover. According to the analysis, the total living biomass varied between 46 and 67 Pg, largely because of different estimates of forest area. Although optical data are limited in distinguishing differences in biomass in closed canopy forests, the estimates of total living biomass obtained here varied more in response to different definitions of forest than to saturation of the optical sensing of biomass.

1 CFR 500.110 - Self-evaluation.

Science.gov (United States)

2010-01-01

... 1 General Provisions 1 2010-01-01 2010-01-01 false Self-evaluation. 500.110 Section 500.110... PROGRAMS OR ACTIVITIES CONDUCTED BY THE NATIONAL COMMISSION FOR EMPLOYMENT POLICY § 500.110 Self-evaluation... or organizations representing handicapped persons, to participate in the self-evaluation process by...

Saturn satellites

International Nuclear Information System (INIS)

Ruskol, E.L.

1981-01-01

The characteristics of the Saturn satellites are discussed. The satellites close to Saturn - Janus, Mimas, Enceladus, Tethys, Dione and Rhea - rotate along the circular orbits. High reflectivity is attributed to them, and the density of the satellites is 1 g/cm 3 . Titan is one of the biggest Saturn satellites. Titan has atmosphere many times more powerful than that of Mars. The Titan atmosphere is a peculiar medium with a unique methane and hydrogen distribution in the whole Solar system. The external satellites - Hyperion, Japetus and Phoebe - are poorly investigated. Neither satellite substance density, nor their composition are known. The experimental data on the Saturn rings obtained on the ''Pioneer-11'' and ''Voyager-1'' satellites are presented [ru

47 CFR 65.500 - Net income.

Science.gov (United States)

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Net income. 65.500 Section 65.500... OF RETURN PRESCRIPTION PROCEDURES AND METHODOLOGIES Interexchange Carriers § 65.500 Net income. The net income methodology specified in § 65.450 shall be utilized by all interexchange carriers that are...

20 CFR 638.500 - Orientation program.

Science.gov (United States)

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Orientation program. 638.500 Section 638.500 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR JOB CORPS PROGRAM UNDER TITLE IV-B OF THE JOB TRAINING PARTNERSHIP ACT Center Operations § 638.500 Orientation program. The...

Modifications of alkaline microgel electrophoresis for sensitive detection of DNA damage

International Nuclear Information System (INIS)

Singh, N.P.; Stephens, R.E.; Schneider, E.L.

1994-01-01

The alkaline microgel electrophoresis technique was modified to achieve a substantial increase in sensitivity for the detection of radiation-induced DNA damage in human lymphocytes. This increased sensitivity was achieved through: (1) the addition of free radical scavengers to the electrophoresis solution to reduce DNA damage generated during alkaline unwinding and electrophoresis; (2) the modification of the electrophoresis unit to achieve a more uniform electric field; (3) the use of YOYO-1, a DNA dye, producing fluorescence 500-fold more intense than ethidium bromide; and (4) the introduction of an image analysis system for the quantitation of DNA migration. In human lymphocytes, these modifications have resulted in an increased sensitivity of several fold, allowing the detection of DNA damage in the range of 50 mGy. (author)

13 CFR 500.202 - Loan amount.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Loan amount. 500.202 Section 500.202 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD EMERGENCY OIL AND GAS GUARANTEED LOAN PROGRAM Oil and Gas Guaranteed Loans § 500.202 Loan amount. The aggregate amount of loan...

31 CFR 500.301 - Foreign country.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Foreign country. 500.301 Section 500.301 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... § 500.301 Foreign country. The term foreign country also includes, but not by way of limitation: (a) The...

Efficient method for the extraction of genomic DNA from wormwood ...

African Journals Online (AJOL)

STORAGESEVER

2008-09-17

Sep 17, 2008 ... DNA is extracted from plant material it will also contain. *Corresponding author. ... an important issue in the field of plant molecular biology. Various plants ..... An excellent guide to over 500 of the more well known medicinal ...

31 CFR 500.412 - Process vs. manufacture.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Process vs. manufacture. 500.412 Section 500.412 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE... Interpretations § 500.412 Process vs. manufacture. A commodity subject to § 500.204 remains subject howsoever it...

13 CFR 500.204 - Loan terms.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Loan terms. 500.204 Section 500.204 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD EMERGENCY OIL AND GAS GUARANTEED LOAN PROGRAM Oil and Gas Guaranteed Loans § 500.204 Loan terms. (a) All loans guaranteed under the...

Transiting Exoplanet Survey Satellite (TESS)

Science.gov (United States)

Ricker, G. R.; Clampin, M.; Latham, D. W.; Seager, S.; Vanderspek, R. K.; Villasenor, J. S.; Winn, J. N.

2012-01-01

The Transiting Exoplanet Survey Satellite (TESS) will discover thousands of exoplanets in orbit around the brightest stars in the sky. In a two-year survey, TESS will monitor more than 500,000 stars for temporary drops in brightness caused by planetary transits. This first-ever spaceborne all-sky transit survey will identify planets ranging from Earth-sized to gas giants, around a wide range of stellar types and orbital distances. No ground-based survey can achieve this feat. A large fraction of TESS target stars will be 30-100 times brighter than those observed by Kepler satellite, and therefore TESS . planets will be far easier to characterize with follow-up observations. TESS will make it possible to study the masses, sizes, densities, orbits, and atmospheres of a large cohort of small planets, including a sample of rocky worlds in the habitable zones of their host stars. TESS will provide prime targets for observation with the James Webb Space Telescope (JWST), as well as other large ground-based and space-based telescopes of the future. TESS data will be released with minimal delay (no proprietary period), inviting immediate community-wide efforts to study the new planets. The TESS legacy will be a catalog of the very nearest and brightest main-sequence stars hosting transiting exoplanets, thus providing future observers with the most favorable targets for detailed investigations.

The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13.

Science.gov (United States)

Fuchs, M; Pinck, M; Serghini, M A; Ravelonandro, M; Walter, B; Pinck, L

1989-04-01

The nucleotide sequence of cDNA copies of grapevine fanleaf virus (strain F13) satellite RNA has been determined. The primary structure obtained was 1114 nucleotides in length, excluding the poly(A) tail, and contained only one long open reading frame encoding a 341 residue, highly hydrophilic polypeptide of Mr37275. The coding sequence was bordered by a leader of 14 nucleotides and a 3'-terminal non-coding region of 74 nucleotides. No homology has been found with small satellite RNAs associated with other nepoviruses. Two limited homologies of eight nucleotides have been detected between the satellite RNA in grapevine fanleaf virus and those in tomato black ring virus, and a consensus sequence U.G/UGAAAAU/AU/AU/A at the 5' end of nepovirus RNAs is reported. A less extended consensus exists in this region in comovirus and picornavirus RNA.

Genome-wide characterization of centromeric satellites from multiple mammalian genomes.

Science.gov (United States)

Alkan, Can; Cardone, Maria Francesca; Catacchio, Claudia Rita; Antonacci, Francesca; O'Brien, Stephen J; Ryder, Oliver A; Purgato, Stefania; Zoli, Monica; Della Valle, Giuliano; Eichler, Evan E; Ventura, Mario

2011-01-01

Despite its importance in cell biology and evolution, the centromere has remained the final frontier in genome assembly and annotation due to its complex repeat structure. However, isolation and characterization of the centromeric repeats from newly sequenced species are necessary for a complete understanding of genome evolution and function. In recent years, various genomes have been sequenced, but the characterization of the corresponding centromeric DNA has lagged behind. Here, we present a computational method (RepeatNet) to systematically identify higher-order repeat structures from unassembled whole-genome shotgun sequence and test whether these sequence elements correspond to functional centromeric sequences. We analyzed genome datasets from six species of mammals representing the diversity of the mammalian lineage, namely, horse, dog, elephant, armadillo, opossum, and platypus. We define candidate monomer satellite repeats and demonstrate centromeric localization for five of the six genomes. Our analysis revealed the greatest diversity of centromeric sequences in horse and dog in contrast to elephant and armadillo, which showed high-centromeric sequence homogeneity. We could not isolate centromeric sequences within the platypus genome, suggesting that centromeres in platypus are not enriched in satellite DNA. Our method can be applied to the characterization of thousands of other vertebrate genomes anticipated for sequencing in the near future, providing an important tool for annotation of centromeres.

31 CFR 500.320 - Domestic bank.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Domestic bank. 500.320 Section 500... § 500.320 Domestic bank. The term domestic bank shall mean any branch or office within the United States of any of the following which is not a national of any designated foreign country: Any bank or trust...

Homologous alpha satellite sequences on human acrocentric chromosomes with selectivity for chromosomes 13, 14, and 21: implications for recombination between nonhomologues and Robertsonian translocations

Energy Technology Data Exchange (ETDEWEB)

Choo, K H; Vissel, B; Brown, R; Filby, R G; Earle, E

1988-02-25

The authors report a new subfamily of alpha satellite DNA (pTRA-2) which is found on all the human acrocentric chromosomes. The alphoid nature of the cloned DNA was established by partial sequencing. Southern analysis of restriction enzyme-digested DNA fragments from mouse/human hybrid cells containing only human chromosome 21 showed that the predominant higher-order repeating unit for pTRA-2 is a 3.9 kb structure. Analysis of a consensus in situ hybridization profile derived from 13 normal individuals revealed the localization of 73% of all centromeric autoradiographic grains over the five acrocentric chromosomes, with the following distribution: 20.4%, 21.5%, 17.1%, 7.3% and 6.5% on chromosomes 13, 14, 21, 15 and 22 respectively. An average of 1.4% of grains was found on the centromere of each of the remaining 19 nonacrocentric chromosomes. These results indicate the presence of a common subfamily of alpha satellite DNA on the five acrocentric chromosomes and suggest an evolutionary process consistent with recombination exchange of sequences between the nonhomologues. The results further suggests that such exchanges are more selective for chromosomes 13, 14 and 21 than for chromosomes 15 and 22. The possible role of centromeric alpha satellite DNA in the aetiology of 13q14q and 14q21q Robertsonian translocation involving the common and nonrandom association of chromosomes 13 and 14, and 14 and 21 is discussed.

Improving enrichment of circulating fetal DNA for genetic testing: size fractionation followed by whole gene amplification.

Science.gov (United States)

Jorgez, Carolina J; Bischoff, Farideh Z

2009-01-01

Among the pitfalls of using cell-free fetal DNA in plasma for prenatal diagnosis is quality of the recovered DNA fragments and concomitant presence of maternal DNA (>95%). Our objective is to provide alternative methods for achieving enrichment and high-quality fetal DNA from plasma. Cell-free DNA from 31 pregnant women and 18 controls (10 males and 8 females) were size separated using agarose gel electrophoresis. DNA fragments of 100-300, 500-700 and 1,500-2,000 bp were excised and extracted, followed by whole genome amplification (WGA) of recovered fragments. Levels of beta-globin and DYS1 were measured. Distribution of beta-globin size fragments was similar among pregnant women and controls. Among control male cases, distribution of size fragments was the same for both beta-globin and DYS1. Among maternal cases confirmed to be male, the smallest size fragment (100-300 bp) accounted for nearly 50% (39.76 +/- 17.55%) of the recovered DYS1-DNA (fetal) and only 10% (10.40 +/- 6.49%) of beta-globin (total) DNA. After WGA of plasma fragments from pregnant women, DYS1 sequence amplification was best observed when using the 100-300 bp fragments as template. Combination of electrophoresis for size separation and WGA led to enriched fetal DNA from plasma. This novel combination of strategies is more likely to permit universal clinical applications of cell-free fetal DNA. Copyright 2009 S. Karger AG, Basel.

CpSAT-1, a transcribed satellite sequence from the codling moth, Cydia pomonella

Czech Academy of Sciences Publication Activity Database

Věchtová, Pavlína; Dalíková, Martina; Sýkorová, Miroslava; Žurovcová, Martina; Füssy, Zoltán; Zrzavá, Magda

2016-01-01

Roč. 144, č. 4 (2016), s. 385-395 ISSN 0016-6707 R&D Projects: GA ČR GA523/09/2106; GA ČR(CZ) GA14-22765S Institutional support: RVO:60077344 Keywords : Cydia pomonella * satellite DNA * holokinetic chromosomes * sex chromosomes * Lepidoptera Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.207, year: 2016

21 CFR 500.92 - Implementation.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Implementation. 500.92 Section 500.92 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS GENERAL Regulation of Carcinogenic Compounds Used in Food-Producing Animals...

31 CFR 500.310 - Transfer.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Transfer. 500.310 Section 500.310 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF FOREIGN..., or other fiduciary; the creation or transfer of any lien; the issuance, docketing, filing, or the...

13 CFR 500.212 - Liquidation.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Liquidation. 500.212 Section 500.212 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD EMERGENCY OIL AND GAS... proper, consistent with Federal law and regulations. (b) Pursuant to the Guarantee, upon written demand...

Genetic Diversity and Population Structure of a Threatened African Tree Species, Milicia excelsa, Using Nuclear Micro satellites DNA Markers

International Nuclear Information System (INIS)

Ouinsavi, Ch.; Sokpon, N.; Ouinsavi, Ch.; Khasa, D.P.

2009-01-01

To accurately estimate the genetic diversity and population structure for improved conservation planning of Milicia excelsa tree, 212 individuals from twelve population samples covering the species' range in Benin were surveyed at seven specific micro satellite DNA loci. All loci were variable, with the mean number of alleles per locus ranging from 5.86 to 7.69. Considerable genetic variability was detected for all populations at the seven loci (AR=4.60; HE=0.811). Moderate but statistically significant genetic differentiation was found among populations considering both FST (0.112) and RST (0.342). All of the populations showed heterozygosity deficits in test of Hardy-Weinberg Equilibrium and significantly positive FIS values due to inbreeding occurring in the species. Pairwise FST values were positively and significantly correlated with geographical distances (r=0.432; P=.007, Mantel's test) indicating that populations are differentiated by isolation by distance. Bayesian analysis of population structure showed division of the genetic variation into four clusters revealing the existence of heterogeneity in population genetic structure. Altogether, these results indicate that genetic variation in Milicia excelsa is geographically structured. Information gained from this study also emphasized the need for in situ conservation of the relict populations and establishment of gene flow corridors through agroforestry systems for interconnecting these remnant populations.

31 CFR 500.309 - Transactions.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Transactions. 500.309 Section 500.309 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF FOREIGN..., but not by way of limitation: (a) Any payment or transfer to any such designated foreign country or...

Search for Best Astronomical Observatory Sites in the MENA Region using Satellite Measurements

International Nuclear Information System (INIS)

Abdelaziz, G; Guebsi, R; Flamant, C; Guessoum, N

2017-01-01

We perform a systematic search for astronomical observatory sites in the MENA (Middle-East and North Africa) region using space-based data for all the relevant factors, i.e. altitude (DEM), cloud fraction (CF), light pollution (NTL), precipitable water vapor (PWV), aerosol optical depth (AOD), relative humidity (RH), wind speed (WS), Richardson Number (RN), and diurnal temperature range (DTR). We look for the best locations overall even where altitudes are low (the threshold that we normally consider being 1,500 m) or where the combination of the afore-mentioned determining factors had previously excluded all locations in a given country. In this aim, we use the rich data that Earth-observing satellites provide, e.g. the Terra and Aqua multi-national NASA research satellites, with their MODIS (Moderate Resolution Imaging Spectroradiometer) and AIRS (Atmospheric Infrared Sounder) instruments, the Defense Meteorological Satellite Program’s Operational Linescan System (DMSP-OLS), and other products from climate diagnostics archives (e.g. MERRA). We present preliminary results on the best locations for the region. (paper)

Search for Best Astronomical Observatory Sites in the MENA Region using Satellite Measurements

Science.gov (United States)

Abdelaziz, G.; Guebsi, R.; Guessoum, N.; Flamant, C.

2017-06-01

We perform a systematic search for astronomical observatory sites in the MENA (Middle-East and North Africa) region using space-based data for all the relevant factors, i.e. altitude (DEM), cloud fraction (CF), light pollution (NTL), precipitable water vapor (PWV), aerosol optical depth (AOD), relative humidity (RH), wind speed (WS), Richardson Number (RN), and diurnal temperature range (DTR). We look for the best locations overall even where altitudes are low (the threshold that we normally consider being 1,500 m) or where the combination of the afore-mentioned determining factors had previously excluded all locations in a given country. In this aim, we use the rich data that Earth-observing satellites provide, e.g. the Terra and Aqua multi-national NASA research satellites, with their MODIS (Moderate Resolution Imaging Spectroradiometer) and AIRS (Atmospheric Infrared Sounder) instruments, the Defense Meteorological Satellite Program’s Operational Linescan System (DMSP-OLS), and other products from climate diagnostics archives (e.g. MERRA). We present preliminary results on the best locations for the region.

Digital optical feeder links system for broadband geostationary satellite

Science.gov (United States)

Poulenard, Sylvain; Mège, Alexandre; Fuchs, Christian; Perlot, Nicolas; Riedi, Jerome; Perdigues, Josep

2017-02-01

An optical link based on a multiplex of wavelengths at 1.55μm is foreseen to be a valuable solution for the feeder link of the next generation of high-throughput geostationary satellite. The main satellite operator specifications for such link are an availability of 99.9% over the year, a capacity around 500Gbit/s and to be bent-pipe. Optical ground station networks connected to Terabit/s terrestrial fibers are proposed. The availability of the optical feeder link is simulated over 5 years based on a state-of-the-art cloud mask data bank and an atmospheric turbulence strength model. Yearly and seasonal optical feeder link availabilities are derived and discussed. On-ground and on-board terminals are designed to be compliant with 10Gbit/s per optical channel data rate taking into account adaptive optic systems to mitigate the impact of atmospheric turbulences on single-mode optical fiber receivers. The forward and return transmission chains, concept and implementation, are described. These are based on a digital transparent on-off keying optical link with digitalization of the DVB-S2 and DVB-RCS signals prior to the transmission, and a forward error correcting code. In addition, the satellite architecture is described taking into account optical and radiofrequency payloads as well as their interfaces.

44 CFR 19.500 - Employment.

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Employment. 19.500 Section 19... FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 19.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded...

13 CFR 113.500 - Employment.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Employment. 113.500 Section 113... Discrimination on the Basis of Sex in Employment in Education Programs Or Activities Prohibited § 113.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

36 CFR 1211.500 - Employment.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Employment. 1211.500 Section... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1211.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

18 CFR 1317.500 - Employment.

Science.gov (United States)

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Employment. 1317.500... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 1317.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

38 CFR 23.500 - Employment.

Science.gov (United States)

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Employment. 23.500... Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 23.500 Employment. (a) General. (1) No person shall, on the basis of sex, be excluded from participation in, be...

Enhanced throughput for infrared automated DNA sequencing

Science.gov (United States)

Middendorf, Lyle R.; Gartside, Bill O.; Humphrey, Pat G.; Roemer, Stephen C.; Sorensen, David R.; Steffens, David L.; Sutter, Scott L.

1995-04-01

Several enhancements have been developed and applied to infrared automated DNA sequencing resulting in significantly higher throughput. A 41 cm sequencing gel (31 cm well- to-read distance) combines high resolution of DNA sequencing fragments with optimized run times yielding two runs per day of 500 bases per sample. A 66 cm sequencing gel (56 cm well-to-read distance) produces sequence read lengths of up to 1000 bases for ds and ss templates using either T7 polymerase or cycle-sequencing protocols. Using a multichannel syringe to load 64 lanes allows 16 samples (compatible with 96-well format) to be visualized for each run. The 41 cm gel configuration allows 16,000 bases per day (16 samples X 500 bases/sample X 2 ten hour runs/day) to be sequenced with the advantages of infrared technology. Enhancements to internal labeling techniques using an infrared-labeled dATP molecule (Boehringer Mannheim GmbH, Penzberg, Germany; Sequenase (U.S. Biochemical) have also been made. The inclusion of glycerol in the sequencing reactions yields greatly improved results for some primer and template combinations. The inclusion of (alpha) -Thio-dNTP's in the labeling reaction increases signal intensity two- to three-fold.

Hybrid 3D structures of ZnO nanoflowers and PdO nanoparticles as a highly selective methanol sensor.

Science.gov (United States)

Acharyya, D; Huang, K Y; Chattopadhyay, P P; Ho, M S; Fecht, H-J; Bhattacharyya, P

2016-05-10

The present study concerns the enhancement of methanol selectivity of three dimensional (3D) nanoflowers (NFs) of ZnO by dispersing nickel oxide (NiO) and palladium oxide (PdO) nanoparticles on the surface of the nanoflowers to form localized hybrid nano-junctions. The nanoflowers were fabricated through a liquid phase deposition technique and the modification was achieved by addition of NiCl and PdCl2 solutions. In addition to the detailed structural (like X-ray diffraction (XRD), electron dispersive spectroscopy (EDS), X-ray mapping, XPS) and morphological characterization (by field emission scanning electron microscopy (FESEM)), the existence of different defect states (viz. oxygen vacancy) was also confirmed by photoluminescence (PL) spectroscopy. The sensing properties of the pristine and metal oxide nanoparticle (NiO/PdO)-ZnO NF hybrid sensor structures, towards different alcohol vapors (methanol, ethanol, 2-propanol) were investigated in the concentration range of 0.5-700 ppm at 100-350 °C. Methanol selectivity study against other interfering species, viz. ethanol, 2-propanol, acetone, benzene, xylene and toluene was also investigated. It was found that the PdO-ZnO NF hybrid system offered enhanced selectivity towards methanol at low temperature (150 °C) compared to the NiO-ZnO NF and pristine ZnO NF counterparts. The underlying mechanism for such improvement has been discussed with respective energy band diagram and preferential dissociation of target species on such 3D hybrid structures. The corresponding improvement in transient characteristics has also been co-related with the proposed model.

7 CFR 500.3 - Preservation of property.

Science.gov (United States)

2010-01-01

... 7 Agriculture 6 2010-01-01 2010-01-01 false Preservation of property. 500.3 Section 500.3 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL RESEARCH SERVICE, DEPARTMENT OF AGRICULTURE NATIONAL ARBORETUM Conduct on U.S. National Arboreturm Property § 500.3 Preservation...

10 CFR 600.500 - Purpose and scope.

Science.gov (United States)

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Purpose and scope. 600.500 Section 600.500 Energy DEPARTMENT OF ENERGY (CONTINUED) ASSISTANCE REGULATIONS FINANCIAL ASSISTANCE RULES Eligibility Determination for Certain Financial Assistance Programs-General Statement of Policy § 600.500 Purpose and scope...

29 CFR 500.80 - Payroll records required.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Payroll records required. 500.80 Section 500.80 Labor... SEASONAL AGRICULTURAL WORKER PROTECTION Worker Protections Wages and Payroll Standards § 500.80 Payroll... agricultural association which employs any migrant or seasonal agricultural worker shall preserve all payroll...

31 CFR 500.578 - Vietnamese property unblocked.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Vietnamese property unblocked. 500.578 Section 500.578 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued..., Authorizations and Statements of Licensing Policy § 500.578 Vietnamese property unblocked. All transactions...

Use of a D17Z1 oligonucleotide probe for human DNA quantitation prior to PCR analysis of polymorphic DNA markers

Energy Technology Data Exchange (ETDEWEB)

Walsh, S.; Alavaren, M.; Varlaro, J. [Roche Molecular Systems, Alameda, CA (United States)] [and others

1994-09-01

The alpha-satellite DNA locus D17Z1 contains primate-specific sequences which are repeated several hundred times per chromosome 17. A probe that was designed to hybridize to a subset of the D17Z1 sequence can be used for very sensitive and specific quantitation of human DNA. Sample human genomic DNA is immobilized on nylon membrane using a slot blot apparatus, and then hybridized with a biotinylated D17Z1 oligonucleotide probe. The subsequent binding of streptavidin-horseradish peroxidase to the bound probe allows for either calorimetric (TMB) or chemiluminescent (ECL) detection. Signals obtained for sample DNAs are then compared to the signals obtained for a series of human DNA standards. For either detection method, forty samples can be quantitated in less than two hours, with a sensitivity of 150 pg. As little as 20 pg of DNA can be quantitated when using chemiluminescent detection with longer film exposures. PCR analysis of several VNTR and STR markers has indicated that optimal typing results are generally obtained within a relatively narrow range of input DNA quantities. Too much input DNA can lead to PCR artifacts such as preferential amplification of smaller alleles, non-specific amplification products, and exaggeration of the DNA synthesis slippage products that are seen with STR markers. Careful quantitation of human genomic DNA prior to PCR can avoid or minimize these problems and ultimately give cleaner, more unambiguous PCR results.

29 CFR 500.100 - Vehicle safety obligations.

Science.gov (United States)

2010-07-01

... safety standards. Prima facie evidence that safety standards have been met will be shown by the presence... 29 Labor 3 2010-07-01 2010-07-01 false Vehicle safety obligations. 500.100 Section 500.100 Labor... § 500.100 Vehicle safety obligations. (a) General obligations. Each farm labor contractor, agricultural...

48 CFR 504.500 - Scope of subpart.

Science.gov (United States)

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Scope of subpart. 504.500 Section 504.500 Federal Acquisition Regulations System GENERAL SERVICES ADMINISTRATION GENERAL ADMINISTRATIVE MATTERS Electronic Commerce in Contracting 504.500 Scope of subpart. This subpart provides policy and procedure for use of GSA's Electronic...

16 CFR 500.4 - Statement of identity.

Science.gov (United States)

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Statement of identity. 500.4 Section 500.4... PACKAGING AND LABELING ACT § 500.4 Statement of identity. (a) The principal display panel of a consumer commodity shall bear a specification of the identity of the commodity. (b) Such specification of identity...

Using Life Cycle Assessment to identify potential environmental impacts of an agrifood sector: Application to the PDO Beaujolais and Burgundia wine sector

Directory of Open Access Journals (Sweden)

Penavayre Sophie

2016-01-01

Full Text Available The environmental impacts of the production system of emblematic French product under official quality marks was investigated using the Life Cycle Assessment (LCA methodology. The study looks at the PDO Beaujolais and Burgundy sector from a broad perspective, i.e. encompassing all steps linked with the products themselves but also complementary activities that belong to this wine sector. To build the Life Cycle Inventory (LCI, a methodology deriving from both product and organizational LCA was developed and applied. The LCI was built using a bottom-up approach. Inventories were first built for a sample of 17 representative companies. Then, these inventories were scaled-up to complete the global LCI at the agrifood sector level. Potential environmental impacts were assessed for 8 indicators. The LCA results show potential environmental impacts for each life cycle step: grape production, wine making and aging, packaging, distribution and activity of stakeholders belonging to the “close environment”. It provided two main outcomes: (i a methodology for the construction of an LCI adapted to the perimeter of an agrifood sector and composed by high quality data; and (ii the identification of potential environmental impacts of the studied agrifood sector, providing assistance for the definition of their strategic orientations for the future.

Formation of Hydroxymethyl DNA Adducts in Rats Orally Exposed to Stable Isotope Labeled Methanol

Science.gov (United States)

Lu, Kun; Gul, Husamettin; Upton, Patricia B.; Moeller, Benjamin C.; Swenberg, James A.

2012-01-01

Methanol is a large volume industrial chemical and widely used solvent and fuel additive. Methanol’s well known toxicity and use in a wide spectrum of applications has raised long-standing environmental issues over its safety, including its carcinogenicity. Methanol has not been listed as a carcinogen by any regulatory agency; however, there are debates about its carcinogenic potential. Formaldehyde, a metabolite of methanol, has been proposed to be responsible for the carcinogenesis of methanol. Formaldehyde is a known carcinogen and actively targets DNA and protein, causing diverse DNA and protein damage. However, formaldehyde-induced DNA adducts arising from the metabolism of methanol have not been reported previously, largely due to the absence of suitable DNA biomarkers and the inability to differentiate what was due to methanol compared with the substantial background of endogenous formaldehyde. Recently, we developed a unique approach combining highly sensitive liquid chromatography-mass spectrometry methods and exposure to stable isotope labeled chemicals to simultaneously quantify formaldehyde-specific endogenous and exogenous DNA adducts. In this study, rats were exposed daily to 500 or 2000 mg/kg [13CD4]-methanol by gavage for 5 days. Our data demonstrate that labeled formaldehyde arising from [13CD4]-methanol induced hydroxymethyl DNA adducts in multiple tissues in a dose-dependent manner. The results also demonstrated that the number of exogenous DNA adducts was lower than the number of endogenous hydroxymethyl DNA adducts in all tissues of rats administered 500 mg/kg per day for 5 days, a lethal dose to humans, even after incorporating an average factor of 4 for reduced metabolism due to isotope effects of deuterium-labeled methanol into account. PMID:22157354

An analysis of surface air temperature trends and variability along the Andes

Science.gov (United States)

Franquist, Eric S.

Climate change is difficult to study in mountainous regions such as the Andes since steep changes in elevation cannot always be resolved by climate models. However, it is important to examine temperature trends in this region as rises in surface air temperature are leading to the melting of tropical glaciers. Local communities rely on the glacier-fed streamflow to get their water for drinking, irrigation, and livestock. Moreover, communities also rely on the tourism of hikers who come to the region to view the glaciers. As the temperatures increase, these glaciers are no longer in equilibrium with their current climate and are receding rapidly and decreasing the streamflow. This thesis examines surface air temperature from 858 weather stations across Ecuador, Peru, and Chile in order to analyze changes in trends and variability. Three time periods were studied: 1961--1990, 1971--2000, and 1981--2010. The greatest warming occurred during the period of 1971--2000 with 92% of the stations experiencing positive trends with a mean of 0.24°C/decade. There was a clear shift toward cooler temperatures at all latitudes and below elevations of 500 m during the most recent time period studied (1981--2010). Station temperatures were more strongly correlated with the El Nino Southern Oscillation (ENSO), than the Pacific Decadal Oscillation (PDO), and the Southern Annular Mode (SAM). A principal component analysis confirmed ENSO as the main contributor of variability with the most influence in the lower latitudes. There were clear multidecadal changes in correlation strength for the PDO. The PDO contributed the most to the increases in station temperature trends during the 1961--1990 period, consistent with the PDO shift to the positive phase in the middle of this period. There were many strong positive trends at individual stations during the 1971--2000 period; however, these trends could not fully be attributed to ENSO, PDO, or SAM, indicating anthropogenic effects of

Leveraging the NPS Femto Satellite for Alternative Satellite Communication Networks

Science.gov (United States)

2017-09-01

programmed for eventual integration with the Iridium Network , which is then tested. C. THESIS ORGANIZATION The thesis addresses these questions...NPS FEMTO SATELLITE FOR ALTERNATIVE SATELLITE COMMUNICATION NETWORKS by Faisal S. Alshaya September 2017 Co-Advisors: Steven J. Iatrou...TYPE AND DATES COVERED Master’s thesis 4. TITLE AND SUBTITLE LEVERAGING THE NPS FEMTO SATELLITE FOR ALTERNATIVE SATELLITE COMMUNICATION NETWORKS 5

Dark Matter Constraints from Observations of 25 Milky Way Satellite Galaxies with the Fermi Large Area Telescope

Science.gov (United States)

Ackermann, M.; Albert, A.; Anderson, B.; Baldini, L.; Ballet, J.; Barbiellini, G.; Bastieri, D.; Bechtol, K.; Bellazzini, R.; Bissaldi, E.;

12 CFR 411.500 - Secretary of Defense.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Secretary of Defense. 411.500 Section 411.500 Banks and Banking EXPORT-IMPORT BANK OF THE UNITED STATES NEW RESTRICTIONS ON LOBBYING Exemptions § 411.500 Secretary of Defense. (a) The Secretary of Defense may exempt, on a case-by-case basis, a covered...

Meteorological satellite systems

CERN Document Server

Tan, Su-Yin

2014-01-01

“Meteorological Satellite Systems” is a primer on weather satellites and their Earth applications. This book reviews historic developments and recent technological advancements in GEO and polar orbiting meteorological satellites. It explores the evolution of these remote sensing technologies and their capabilities to monitor short- and long-term changes in weather patterns in response to climate change. Satellites developed by various countries, such as U.S. meteorological satellites, EUMETSAT, and Russian, Chinese, Japanese and Indian satellite platforms are reviewed. This book also discusses international efforts to coordinate meteorological remote sensing data collection and sharing. This title provides a ready and quick reference for information about meteorological satellites. It serves as a useful tool for a broad audience that includes students, academics, private consultants, engineers, scientists, and teachers.

Molecular dynamics of formation of TD lesioned DNA complexed with repair enzyme - onset of the enzymatic repair process

Energy Technology Data Exchange (ETDEWEB)

Pinak, Miroslav [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

1999-12-01

To describe the first step of the enzymatic repair process (formation of complex enzyme-DNA), in which the thymine dimer (TD) part is removed from DNA, the 500 picosecond (ps) molecular dynamics (MD) simulation of TD lesioned DNA and part of repair enzyme cell (inclusive of catalytic center - Arg-22, Glu-23, Arg-26 and Thr-2) was performed. TD is UV originated lesion in DNA and T4 Endonuclease V is TD specific repair enzyme. Both molecules were located in the same simulation cell and their relative movement was examined. During the simulation the research was focused on the role of electrostatic energy in formation of complex enzyme-DNA. It is found, that during the first 100 ps of MD, the part of enzyme approaches the DNA surface at the TD lesion, interacts extensively by electrostatic and van der Walls interactions with TD part of DNA and forms complex that lasts stabile for 500 ps of MD. In the beginning of MD, the positive electrostatic interaction energy between part of enzyme and TD ({approx} +10 kcal/mol) drives enzyme towards the DNA molecule. Water-mediated hydrogen bonds between enzyme and DNA help to keep complex stabile. As a reference, the MD simulation of the identical system with native DNA molecule (two native thymines (TT) instead of TD) was performed. In this system the negative electrostatic interaction energy between part of enzyme and TT ({approx} -11 kcal/mol), in contrary to the positive one in the system with TD, doesn't drive enzyme towards DNA and complex is not formed. (author)

Molecular dynamics of formation of TD lesioned DNA complexed with repair enzyme - onset of the enzymatic repair process

International Nuclear Information System (INIS)

Pinak, Miroslav

1999-12-01

To describe the first step of the enzymatic repair process (formation of complex enzyme-DNA), in which the thymine dimer (TD) part is removed from DNA, the 500 picosecond (ps) molecular dynamics (MD) simulation of TD lesioned DNA and part of repair enzyme cell (inclusive of catalytic center - Arg-22, Glu-23, Arg-26 and Thr-2) was performed. TD is UV originated lesion in DNA and T4 Endonuclease V is TD specific repair enzyme. Both molecules were located in the same simulation cell and their relative movement was examined. During the simulation the research was focused on the role of electrostatic energy in formation of complex enzyme-DNA. It is found, that during the first 100 ps of MD, the part of enzyme approaches the DNA surface at the TD lesion, interacts extensively by electrostatic and van der Walls interactions with TD part of DNA and forms complex that lasts stabile for 500 ps of MD. In the beginning of MD, the positive electrostatic interaction energy between part of enzyme and TD (∼ +10 kcal/mol) drives enzyme towards the DNA molecule. Water-mediated hydrogen bonds between enzyme and DNA help to keep complex stabile. As a reference, the MD simulation of the identical system with native DNA molecule (two native thymines (TT) instead of TD) was performed. In this system the negative electrostatic interaction energy between part of enzyme and TT (∼ -11 kcal/mol), in contrary to the positive one in the system with TD, doesn't drive enzyme towards DNA and complex is not formed. (author)

IAEA Safeguards: Cost/benefit analysis of commercial satellite imagery

International Nuclear Information System (INIS)

Andersson, Christer

1999-03-01

feasible undertaking. Such a unit capable of performing advanced image processing as a tool for various safeguard tasks will give the Agency an effective instrument for reference, monitoring, verification, and detection of declared and undeclared activities. It is also demonstrated in the study that more than 20% of the envisaged work tasks in all of the major Safeguards applications will achieve a better decision support from the use of commercial satellite imagery. At the same time the potential savings in costs is calculated to approximately USD 500,000 per year by reductions in on-site inspections and by more efficient planning and logistics. The total cost for implementing commercial satellite imagery at the Department for Safeguards, as simulated in this study, is approximately USD 1,500,000 per year. This cost rises when utilising the full potential of high-resolution imagery in all five applications including monitoring and detection of undeclared facilities. The cost/benefit simulation is founded on an activity scenario with a staff of 4 experts working in an IAEA imagery unit with a workload of three dossiers or 'issues' per week. The imagery unit is built around an advanced workstation PC image processing system capable of handling several hundreds of pre-processed imagery per year

Raman microspectroscopic study on low-pH-induced DNA structural transitions in the presence of magnesium ions

NARCIS (Netherlands)

Muntean, C.M.; Puppels, G.J.; Greve, Jan; Segers-Nolten, Gezina M.J.; Cinta-Pinzaru, S.

2002-01-01

Low-pH-induced DNA structural changes were investigated in the pH range 6.8-2.10 by Raman microspectroscopy. Measurements were carried out on calf thymus DNA in the presence of low concentrations of Mg2+ ions. Vibrational spectra are presented in the wavenumber region 500-1650 cm-1. Large changes in

78 FR 75511 - Special Conditions: Bombardier Inc., Models BD-500-1A10 and BD-500-1A11 Series Airplanes...

Science.gov (United States)

2013-12-12

... Inc., Models BD-500-1A10 and BD- 500-1A11 Series Airplanes; Electronic Flight Control System: Control... Inc. Models BD-500-1A10 and BD-500-1A11 series airplanes. These airplanes will have a novel or unusual... comments, data, or views. The most helpful comments reference a specific portion of the special conditions...

77 FR 69568 - Special Conditions: Bombardier Aerospace, Model BD-500-1A10 and BD-500-1A11 Airplanes; Sidestick...

Science.gov (United States)

2012-11-20

... Aerospace, Model BD-500-1A10 and BD-500-1A11 Airplanes; Sidestick Controllers AGENCY: Federal Aviation... conditions for the Bombardier Aerospace Model BD-500-1A10 and BD-500-1A11 airplanes. These airplanes will... on the comments we receive. Background On December 10, 2009, Bombardier Aerospace applied for a type...

Boomerang Satellites

Science.gov (United States)

Hesselbrock, Andrew; Minton, David A.

2017-10-01

We recently reported that the orbital architecture of the Martian environment allows for material in orbit around the planet to ``cycle'' between orbiting the planet as a ring, or as coherent satellites. Here we generalize our previous analysis to examine several factors that determine whether satellites accreting at the edge of planetary rings will cycle. In order for the orbiting material to cycle, tidal evolution must decrease the semi-major axis of any accreting satellites. In some systems, the density of the ring/satellite material, the surface mass density of the ring, the tidal parameters of the system, and the rotation rate of the primary body contribute to a competition between resonant ring torques and tidal dissipation that prevent this from occurring, either permanently or temporarily. Analyzing these criteria, we examine various bodies in our solar system (such as Saturn, Uranus, and Eris) to identify systems where cycling may occur. We find that a ring-satellite cycle may give rise to the current Uranian ring-satellite system, and suggest that Miranda may have formed from an early, more massive Uranian ring.

Complementation of radiation-sensitive Ataxia telangiectasia cells after transfection of cDNA expression libraries and cosmid clones from wildtype cells

International Nuclear Information System (INIS)

Fritz, E.

1994-06-01

In this Ph.D.-thesis, phenotypic complementation of AT-cells (AT5BIVA) by transfection of cDNA-expression-libraries was adressed: After stable transfection of cDNA-expression-libraries G418 resistant clones were selected for enhanced radioresistance by a fractionated X-ray selection. One surviving transfectant clone (clone 514) exhibited enhanced radiation resistance in dose-response experiments and further X-ray selections. Cell cycle analysis revealed complementation of untreated and irradiated 514-cells in cell cycle progression. The rate of DNA synthesis, however, is not diminished after irradiation but shows the reverse effect. A transfected cDNA-fragment (AT500-cDNA) was isolated from the genomic DNA of 514-cells and proved to be an unknown DNA sequence. A homologous sequence could be detected in genomic DNA from human cell lines, but not in DNA from other species. The cDNA-sequence could be localized to human chromosome 11. In human cells the cDNA sequence is part of two large mRNAs. 4 different cosmid clones containing high molecular genomic DNA from normal human cells could be isolated from a library, each hybridizing to the AT500-cDNA. After stable transfection into AT-cells, one cosmid-clone was able to confer enhanced radiation resistance both in X-ray selections and dose-response experiments. The results indicate that the cloned cDNA-fragment is based on an unknown gene from human chromosome 11 which partially complements the radiosensitivity and the defective cell cycle progression in AT5BIVA cells. (orig.) [de

Differential amplification of satellite PaB6 in chromosomally hypervariable Prospero autumnale complex (Hyacinthaceae)

Czech Academy of Sciences Publication Activity Database

Emadzade, K.; Jang, T.S.; Macas, Jiří; Kovařík, Aleš; Novák, Petr; Parker, J.; Weiß-Schneeweiss, H.

2014-01-01

Roč. 114, č. 8 (2014), s. 1597-1608 ISSN 0305-7364 R&D Projects: GA ČR GBP501/12/G090 Institutional support: RVO:60077344 ; RVO:68081707 Keywords : Hyacinthaceae * PaB6 * pericentric satellite DNA * next-generation sequencing * Prospero autumnale Subject RIV: EB - Genetics ; Molecular Biology; EB - Genetics ; Molecular Biology (BFU-R) Impact factor: 3.654, year: 2014

Status of the HTR 500 design program

International Nuclear Information System (INIS)

Baust, E.; Arndt, E.

1987-01-01

Since 1982 BBC/HRB have offered the HTR 500 as the follow-on project of the THTR 300, the first large pebble bed reactor. The technical concept of the HTR-500 largely corresponds to the THTR 300 which has been in operation for almost 2 years now. In developing the design concept of the HTR 500 the ideas and demands of the reactor users in the FRG interested in the HTR have been taken into consideration to a large extent. In 1982 these potential users formed a working group 'Arbeitsgemeinschaft Hochtemperaturreaktor' (AHR), representing 16 power indusry companies and in early 1983, awarded a contract to HRB to perform a conceptual design study on the HTR 500. Within this conceptual design study BBC/HRB developed the safety concept of the HTR 500, prepared a detailed description of the overall power plant, and performed a cost calculation. These activities were completed in 1984. Based on the positive results of this conceptual design study, BBC/HRB are expecting to be granted a design contract by the users company Hochtemperaturreaktor GmbH (HRG) to establish the final complete design plans and documents for the HTR 500. (author)

AST-500 safety analysis experience

Energy Technology Data Exchange (ETDEWEB)

Falikov, A A; Bakhmetiev, A M; Kuul, V S; Samoilov, O B [OKBM, Nizhny Novgorod (Russian Federation)

1997-09-01

Characteristic AST-type NHR safety features and requirements are described briefly. The main approaches and results of design and beyond-design accidents analyses for the AST-500 NHR, and the results of probabilistic safety assessments are considered. It is concluded that the AST-500 possesses a high safety level in virtue of the development and realization in the design of self-protection, passivity and defence-in-depth principles. (author). 9 refs, 2 figs.

Gigabit Satellite Network for NASA's Advanced Communication Technology Satellite (ACTS)

Science.gov (United States)

Hoder, Douglas; Bergamo, Marcos

1996-01-01

The advanced communication technology satellite (ACTS) gigabit satellite network provides long-haul point-to-point and point-to-multipoint full-duplex SONET services over NASA's ACTS. at rates up to 622 Mbit/s (SONET OC-12), with signal quality comparable to that obtained with terrestrial fiber networks. Data multiplexing over the satellite is accomplished using time-division multiple access (TDMA) techniques coordinated with the switching and beam hopping facilities provided by ACTS. Transmissions through the satellite are protected with Reed-Solomon encoding. providing virtually error-free transmission under most weather conditions. Unique to the system are a TDMA frame structure and satellite synchronization mechanism that allow: (a) very efficient utilization of the satellite capacity: (b) over-the-satellite dosed-loop synchronization of the network in configurations with up to 64 ground stations: and (c) ground station initial acquisition without collisions with existing signalling or data traffic. The user interfaces are compatible with SONET standards, performing the function of conventional SONET multiplexers and. as such. can be: readily integrated with standard SONET fiber-based terrestrial networks. Management of the network is based upon the simple network management protocol (SNMP). and includes an over-the-satellite signalling network and backup terrestrial internet (IP-based) connectivity. A description of the ground stations is also included.

9 CFR 319.500 - Meat pies.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Meat pies. 319.500 Section 319.500... ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND VOLUNTARY INSPECTION AND CERTIFICATION DEFINITIONS AND STANDARDS OF IDENTITY OR COMPOSITION Meat Food Entree Products, Pies, and...

41 CFR 101-4.500 - Employment.

Science.gov (United States)

2010-07-01

... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Employment. 101-4.500... Employment in Education Programs or Activities Prohibited § 101-4.500 Employment. (a) General. (1) No person... subjected to discrimination in employment, or recruitment, consideration, or selection therefor, whether...

Absence of ribosomal DNA amplification in the meroistic (telotrophic) ovary of the large milkweed bug Oncopeltus fasciatus (Dallas) (Hemiptera: Lygaeidae)

Science.gov (United States)

1975-01-01

In the typical meroistic insect ovary, the oocyte nucleus synthesizes little if any RNA. Nurse cells or trophocytes actively synthesize ribosomes which are transported to and accumulated by the oocyte. In the telotrophic ovary a morphological separation exists, the nurse cells being localized at the apical end of each ovariole and communicating with the ooocytes via nutritive cords. In order to determine whether the genes coding for ribosomal RNA (rRNA) are amplified in the telotrophic ovary of the milkweed bug Oncopeltus fasciatus, the percentages of the genome coding for ribosomal RNA in somatic cells, spermatogenic cells, ovarian follicles, and nurse cells were compared. The oocytes and most of the nurse cells of O. fasciatus are uninucleolate. DNA hybridizing with ribosomal RNA is localized in a satellite DNA, the density of which is 1.712 g/cm(-3). The density of main-band DNA is 1.694 g/cm(-3). The ribosomal DNA satellite accounts for approximately 0.2% of the DNA in somatic and gametogenic tissues of both males and females. RNA-DNA hybridization analysis demonstrates that approximately 0.03% of the DNA in somatic tissues, testis, ovarian follicles, and isolated nurse cells hybridizes with ribosomal RNA. The fact that the percentage of DNA hybridizing with rRNA is the same in somatic and in male and female gametogenic tissues indicates that amplification of ribosomal DNA does not occur in nurse cells and that if it occurs in oocytes, it represents less than a 50- fold increase in ribosomal DNA. An increase in total genome DNA accounted by polyploidization appears to provide for increasing the amount of ribosomal DNA in the nurse cells. PMID:1158969

The NASA Earth Science Program and Small Satellites

Science.gov (United States)

Neeck, Steven P.

2015-01-01

Earth's changing environment impacts every aspect of life on our planet and climate change has profound implications on society. Studying Earth as a single complex system is essential to understanding the causes and consequences of climate change and other global environmental concerns. NASA's Earth Science Division (ESD) shapes an interdisciplinary view of Earth, exploring interactions among the atmosphere, oceans, ice sheets, land surface interior, and life itself. This enables scientists to measure global and climate changes and to inform decisions by Government, other organizations, and people in the United States and around the world. The data collected and results generated are accessible to other agencies and organizations to improve the products and services they provide, including air quality indices, disaster prediction and response, agricultural yield projections, and aviation safety. ESD's Flight Program provides the spacebased observing systems and supporting infrastructure for mission operations and scientific data processing and distribution that support NASA's Earth science research and modeling activities. The Flight Program currently has 21 operating Earth observing space missions, including the recently launched Global Precipitation Measurement (GPM) mission, the Orbiting Carbon Observatory-2 (OCO-2), the Soil Moisture Active Passive (SMAP) mission, and the International Space Station (ISS) RapidSCAT and Cloud-Aerosol Transport System (CATS) instruments. The ESD has 22 more missions and instruments planned for launch over the next decade. These include first and second tier missions from the 2007 Earth Science Decadal Survey, Climate Continuity missions to assure availability of key climate data sets, and small-sized competitively selected orbital missions and instrument missions of opportunity belonging to the Earth Venture (EV) Program. Small satellites (500 kg or less) are critical contributors to these current and future satellite missions

HIMAWARI-8 Geostationary Satellite Observation of the Internal Solitary Waves in the South China Sea

Science.gov (United States)

Gao, Q.; Dong, D.; Yang, X.; Husi, L.; Shang, H.

2018-04-01

The new generation geostationary meteorological satellite, Himawari-8 (H-8), was launched in 2015. Its main payload, the Advanced Himawari Imager (AHI), can observe the earth with 10-minute interval and as high as 500-m spatial resolution. This makes the H-8 satellite an ideal data source for marine and atmospheric phenomena monitoring. In this study, the propagation of internal solitary waves (ISWs) in the South China Sea is investigated using AHI imagery time series for the first time. Three ISWs cases were studied at 3:30-8:00 UTC on 30 May, 2016. In all, 28 ISWs were detected and tracked between the time series image pairs. The propagation direction and phase speeds of these ISWs are calculated and analyzed. The observation results show that the properties of ISW propagation not stable and maintains nonlinear during its lifetime. The resultant ISW speeds agree well with the theoretical values estimated from the Taylor-Goldstein equation using Argo dataset. This study has demonstrated that the new generation geostationary satellite can be a useful tool to monitor and investigate the oceanic internal waves.

31 CFR 500.314 - Banking institution.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Banking institution. 500.314 Section 500.314 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... or incidentally in the business of banking, of granting or transferring credits, or of purchasing or...

Dark matter constraints from observations of 25 Milky Way satellite galaxies with the Fermi Large Area Telescope

Energy Technology Data Exchange (ETDEWEB)

Ackermann, M.; et al.

2014-02-11

The dwarf spheroidal satellite galaxies of the Milky Way are some of the most dark-matter-dominated objects known. Due to their proximity, high dark matter content, and lack of astrophysical backgrounds, dwarf spheroidal galaxies are widely considered to be among the most promising targets for the indirect detection of dark matter via gamma rays. Here we report on gamma-ray observations of 25 Milky Way dwarf spheroidal satellite galaxies based on 4 years of Fermi Large Area Telescope (LAT) data. None of the dwarf galaxies are significantly detected in gamma rays, and we present gamma-ray flux upper limits between 500 MeV and 500 GeV. We determine the dark matter content of 18 dwarf spheroidal galaxies from stellar kinematic data and combine LAT observations of 15 dwarf galaxies to constrain the dark matter annihilation cross section. We set some of the tightest constraints to date on the the annihilation of dark matter particles with masses between 2 GeV and 10 TeV into prototypical Standard Model channels. We find these results to be robust against systematic uncertainties in the LAT instrument performance, diffuse gamma-ray background modeling, and assumed dark matter density profile.

Comparison of DNA quantification methodology used in the DNA extraction protocol for the UK Biobank cohort.

Science.gov (United States)

Welsh, Samantha; Peakman, Tim; Sheard, Simon; Almond, Rachael

2017-01-05

UK Biobank is a large prospective cohort study in the UK established by the Medical Research Council (MRC) and the Wellcome Trust to enable approved researchers to investigate the role of genetic factors, environmental exposures and lifestyle in the causes of major diseases of late and middle age. A wide range of phenotypic data has been collected at recruitment and has recently been enhanced by the UK Biobank Genotyping Project. All UK Biobank participants (500,000) have been genotyped on either the UK Biobank Axiom® Array or the Affymetrix UK BiLEVE Axiom® Array and the workflow for preparing samples for genotyping is described. The genetic data is hoped to provide further insight into the genetics of disease. All data, including the genetic data, is available for access to approved researchers. Data for two methods of DNA quantification (ultraviolet-visible spectroscopy [UV/Vis]) measured on the Trinean DropSense™ 96 and PicoGreen®) were compared by two laboratories (UK Biobank and Affymetrix). The sample processing workflow established at UK Biobank, for genotyping on the custom Affymetrix Axiom® array, resulted in high quality DNA (average DNA concentration 38.13 ng/μL, average 260/280 absorbance 1.91). The DNA generated high quality genotype data (average call rate 99.48% and pass rate 99.45%). The DNA concentration measured on the Trinean DropSense™ 96 at UK Biobank correlated well with DNA concentration measured by PicoGreen® at Affymetrix (r = 0.85). The UK Biobank Genotyping Project demonstrated that the high throughput DNA extraction protocol described generates high quality DNA suitable for genotyping on the Affymetrix Axiom array. The correlation between DNA concentration derived from UV/Vis and PicoGreen® quantification methods suggests, in large-scale genetic studies involving two laboratories, it may be possible to remove the DNA quantification step in one laboratory without affecting downstream analyses. This would result in

Centriolar satellites

DEFF Research Database (Denmark)

Tollenaere, Maxim A X; Mailand, Niels; Bekker-Jensen, Simon

2015-01-01

Centriolar satellites are small, microscopically visible granules that cluster around centrosomes. These structures, which contain numerous proteins directly involved in centrosome maintenance, ciliogenesis, and neurogenesis, have traditionally been viewed as vehicles for protein trafficking...... highlight newly discovered regulatory mechanisms targeting centriolar satellites and their functional status, and we discuss how defects in centriolar satellite components are intimately linked to a wide spectrum of human diseases....

Diversity and dynamic of lactic acid bacteria strains during aging of a long ripened hard cheese produced from raw milk and undefined natural starter.

Science.gov (United States)

Pogačić, Tomislav; Mancini, Andrea; Santarelli, Marcela; Bottari, Benedetta; Lazzi, Camilla; Neviani, Erasmo; Gatti, Monica

2013-12-01

The aim of this study was to explore diversity and dynamic of indigenous LAB strains associated with a long ripened hard cheese produced from raw milk and undefined natural starter such as PDO Grana Padano cheese. Samples of milk, curd, natural whey culture and cheeses (2nd, 6th, 9th and 13th months of ripening) were collected from 6 cheese factories in northern Italy. DNA was extracted from each sample and from 194 LAB isolates. tRNA(Ala)-23S rDNA-RFLP was applied to identify isolates. Strain diversity was assessed by (GTG)5 rep-PCR and RAPD(P1)-PCR. Finally, culture-independent LH-PCR (V1-V2 16S-rDNA), was considered to explore structure and dynamic of the microbiota. Grana Padano LAB were represented mainly by Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus delbrueckii, Lactobacillus helveticus and Pediococcus acidilactici, while the structure and dynamic of microbiota at different localities was specific. The strength of this work is to have focused the study on isolates coming from more than one cheese factories rather than a high number of isolates from one unique production. We provided a valuable insight into inter and intraspecies diversity of typical LAB strains during ripening of traditional PDO Grana Padano, contributing to the understanding of specific microbial ecosystem of this cheese. Copyright © 2013 Elsevier Ltd. All rights reserved.

Development of a solar array drive mechanism for micro-satellite platforms

Science.gov (United States)

Galatis, Giorgos; Guo, Jian; Buursink, Jeroen

2017-10-01

Photovoltaic solar array (PVSA) systems are the most widely used method for spacecraft power generation. However, in many satellite missions, the optimum orientation of the PVSA system is not always compatible with that of the payload orientation. Many methods, have been examined in the past to overcome this problem. Up to date, the most widely used active method for large costly satellites is the Solar Array Drive Mechanism (SADM). The SADM serves as the interface between the satellite body and the PVSA subsystem, enabling the decoupling of their spatial orientation. Nonetheless, there exists a research and development gap for such systems regarding low cost micro-satellites. During the literature study of this paper, individual orbital parameters of various micro-satellites have been extracted and compared to the rotational freedom of the corresponding SADMs used. The findings demonstrated that the implemented SADMs are over designed. It is therefore concluded that these components are not tailored made for each spacecraft mission individually, but rather, exhibit a generic design to full fill a majority of mission profiles and requirements. Motivated by the above analysis, the cardinal objective of the current research is to develop a low cost mechanism that will be precisely tailored for the use of a low Earth orbit (LEO) micro-satellite platform orbiting in altitudes of 500 - 1000km . The design of the mechanism may vary from the existing miniaturized SADMs. For example, the preliminary analysis of the current research suggests, that the conventional use of the slip ring system as the electronic transfer unit can be replaced by a seMI Orientation Unit (MIOU). Systems engineering tools for concept generation and selection have been used. In addition, simulation and mathematical modelling have been implemented on component and system level, to accurately predict the behaviour of the system under various modes of operation. The production and system testing of

Quantitative analysis of gene-specific DNA damage in human spermatozoa

International Nuclear Information System (INIS)

Sawyer, Dennis E.; Mercer, Belinda G.; Wiklendt, Agnieszka M.; Aitken, R. John

2003-01-01

Recent studies have suggested that human spermatozoa are highly susceptible to DNA damage induced by oxidative stress. However, a detailed analysis of the precise nature of this damage and the extent to which it affects the mitochondrial and nuclear genomes has not been reported. To induce DNA damage, human spermatozoa were treated in vitro with hydrogen peroxide (H 2 O 2 ; 0-5 mM) or iron (as Fe(II)SO 4 , 0-500 μM). Quantitative PCR (QPCR) was used to measure DNA damage in individual nuclear genes (hprt, β-pol and β-globin) and mitochondrial DNA. Single strand breaks were also assessed by alkaline gel electrophoresis. H 2 O 2 was found to be genotoxic toward spermatozoa at concentrations as high as 1.25 mM, but DNA damage was not detected in these cells with lower concentrations of H 2 O 2 . The mitochondrial genome of human spermatozoa was significantly (P 2 O 2 -induced DNA damage than the nuclear genome. However, both nDNA and mtDNA in human spermatozoa were significantly (P<0.001) more resistant to damage than DNA from a variety of cell lines of germ cell and myoblastoid origin. Interestingly, significant DNA damage was also not detected in human spermatozoa treated with iron. These studies report, for the first time, quantitative measurements of DNA damage in specific genes of male germ cells, and challenge the commonly held belief that human spermatozoa are particularly vulnerable to DNA damage

THE ANALYSIS OF MOISTURE DEFICIT BASED ON MODIS AND LANDSAT SATELLITE IMAGES. CASE STUDY: THE OLTENIA PLAIN

Directory of Open Access Journals (Sweden)

ONȚEL IRINA

2014-03-01

Full Text Available Satellite images are an important source of information to identify and analyse some hazardous climatic phenomena such as the dryness and drought. These phenomena are characterized by scarce rainfall, increased evapotranspiration and high soil moisture deficit. The soil water reserve depletes to the wilting coefficient, soon followed by the pedological drought which has negative effects on vegetation and agricultural productivity. The MODIS satellite images (Moderate Resolution Imaging Spectroradiometer allow the monitoring of the vegetation throughout the entire vegetative period, with a frequency of 1-2 days and with a spatial resolution of 250 m, 500 m and 1 km away. Another useful source of information is the LANDSAT satellite images, with a spatial resolution of 30 m. Based on MODIS and Landsat satellite images, were calculated moisture monitoring index such as SIWSI (Shortwave Infrared Water Stress Index. Consequently, some years with low moisture such as 2000, 2002, 2007 and 2012 could be identified. Spatially, the areas with moisture deficit varied from one year to another all over the whole analised period (2000-2012. The remote sensing results was corelated with Standard Precipitation Anomaly, which gives a measure of the severity of a wet or dry event.

15 CFR 8a.500 - Employment.

Science.gov (United States)

2010-01-01

... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Employment. 8a.500 Section 8a.500 Commerce and Foreign Trade Office of the Secretary of Commerce NONDISCRIMINATION ON THE BASIS OF SEX IN...-sponsored activities, including social or recreational programs; and (10) Any other term, condition, or...

31 CFR 500.307 - Unblocked national.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Unblocked national. 500.307 Section 500.307 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF...” shall not be deemed to suspend in any way the requirements of any section of this chapter relating to...

Interference Mitigation Technique Using Active Spaceborne Sensor Antenna in EESS (Active) and Space Research Service (Active) for Use in 500 MHz Bandwidth Near 9.6 GHz

Science.gov (United States)

Huneycutt, Bryan L.

2005-01-01

This document presents an interference mitigation technique using the active spaceborne sensor SAR3 antenna in the Earth Exploration-Satellite Service (active) and Space Research Service (active) for use in a 500 MHz bandwidth near 9.6 GHz. The purpose of the document is present antenna designs which offer lower sidelobes and faster rolloff in the sidelobes which in turn mitigates the interference to other services from the EESS (active) and SRS (active) sensors.

46 CFR 16.500 - Management Information System requirements.

Science.gov (United States)

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Management Information System requirements. 16.500 Section 16.500 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN CHEMICAL TESTING Management Information System § 16.500 Management Information System requirements. (a...

14 CFR 1203.500 - Use of derivative classification.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Use of derivative classification. 1203.500 Section 1203.500 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION INFORMATION SECURITY PROGRAM Derivative Classification § 1203.500 Use of derivative classification. The application of...

Characterization of grape seed oil from wines with protected denomination of origin (PDO from Spain

Directory of Open Access Journals (Sweden)

Bada, J. C.

2015-09-01

Full Text Available The aim of this study was to determine the composition and characteristics of red grape seed oils (Vitis vinifera L from wines with protected denomination of origin (PDO from Spain. Eight representative varieties of grape seed oils from the Spanish wine Ribera del Duero (Tempranillo, Toro (Tempranillo, Rioja (Garnacha, Valencia (Tempranillo and Cangas (Mencia, Carrasquín, Albarín and Verdejo were studied. The oil content of the seeds ranged from 13.89 to 10.18%, and the moisture was similar for all the seeds. Linoleic acid was the most abundant fatty acid in all samples, representing around 78%, followed by oleic acid with a concentration close 16%, the degree of unsaturation in the grape seed oil was over 90%. β-sitosterol and α-tocopherol were the main sterol and tocopherol, reaching values of 77.31% and 3.82 mg·100 g−1 of oil, respectively. In relation to the tocotrienols, α-tocotrienol was the main tocotrienol and accounted for 13.18 mg·100 g−1 of oil.El objetivo de este estudio consistió en determinar la composición y características de aceites de semillas de uvas rojas (Vitis vinifera L de vinos con denominación de origen protegida (DOP de España. Ocho variedades representativas de aceites de semillas de uvas españolas Ribera del Duero (Tempranillo, Toro (Tempranillo, Rioja (Garnacha, Valencia (Tempranillo y Cangas (Mencia, Carrasquín, Albarín y Verdejo fueron estudiadas. Los contenidos en aceite de las semillas oscilaron entre 13.89 y 10.18%, la humedad fué similar para todas las semillas. El contenido en ácido linoléico fué alto en todos los aceites alcanzando un valor del 78%, seguido del ácido oléico con una concentración cercana al 16%, registrando un grado total de insaturación del 90%. b-sitosterol y α-tocoferol fué el principal esterol y tocoferol, alcanzado niveles del 77.31% y de un 3.82 mg·100 g−1 de aceite respectivamente. En relación a los tocotrienoles, α-tocotrienol fué el mayoritario con

The generation of radiolabeled DNA and RNA probes with polymerase chain reaction

International Nuclear Information System (INIS)

Schowalter, D.B.; Sommer, S.S.

1989-01-01

By including a radioactive triphosphate during polymerase chain reaction (PCR), probes of very high specific activity can be generated. The advantages of PCR labeling include (1) uniform labeling with a specific activity of 5 X 10(9) cpm/micrograms or higher (sensitivity of detection: 0.028 pg of target DNA per 24 h); (2) ease of regulation of both the specific activity and the amount of labeled probe produced; (3) efficient labeling of fragments less than 500 bp; (4) efficient incorporation over a wide range of input DNA template; (5) labeling with subnanogram amounts of input DNA; and (6) direct labeling of genomic DNA. The minimal amount of input DNA allows a virtually unlimited number of PCR labeling reactions to be performed on DNA generated by one amplification under the previously described nonlabeling conditions. This obviates the need for CsCl gradients or other large scale methods of DNA preparation. The above advantages except for the very high specific activity can also be achieved by transcript labeling after an amplification where one or both of PCR primers contain a phage promoter sequence

Communication: Electron ionization of DNA bases

Energy Technology Data Exchange (ETDEWEB)

Rahman, M. A.; Krishnakumar, E., E-mail: ekkumar@tifr.res.in

2016-04-28

No reliable experimental data exist for the partial and total electron ionization cross sections for DNA bases, which are very crucial for modeling radiation damage in genetic material of living cell. We have measured a complete set of absolute partial electron ionization cross sections up to 500 eV for DNA bases for the first time by using the relative flow technique. These partial cross sections are summed to obtain total ion cross sections for all the four bases and are compared with the existing theoretical calculations and the only set of measured absolute cross sections. Our measurements clearly resolve the existing discrepancy between the theoretical and experimental results, thereby providing for the first time reliable numbers for partial and total ion cross sections for these molecules. The results on fragmentation analysis of adenine supports the theory of its formation in space.

29 CFR 500.78 - Information in foreign language.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Information in foreign language. 500.78 Section 500.78... § 500.78 Information in foreign language. Each farm labor contractor, agricultural employer and... English or, as necessary and reasonable, in Spanish or another language common to migrant or seasonal...

Teleconnection among Asian Summer Monsoon, ENSO and PDO revealed by instrumental and historic records as well as an annual resolution stalagmite record from Lianhua Cave, northwestern Hunan, China

Science.gov (United States)

Li, H.; Yin, J.; Yuan, D.; Li, T.

2013-12-01

A 33-cm long aragonite stalagmite, LHN-1, from Lianhua Cave (29°09'N, 109°33'E , 459m a.s.l.) in NW Hunan Province of China has been dated by ICP-MS 230Th/U method. The 14 230Th/U dates with less than ×37 years uncertainties yield excellent chronology, showing that the stalagmite grew continuously over the past 3400 years. The 0.1-mm sampling interval for stable isotope analyses provides annual resolution δ18O and δ13C records of the LHN-1 stalagmite. Up-to-dated, a total of 887 measurements for δ18O and δ13C have been made, which reveal detailed monsoonal climatic changes during the past 1000 years. The average values of δ18O and δ13C are -5.83 (‰, PDB) and -3.29 (‰, PDB), respectively. For the youngest part of the δ18O record, we have compared with the instrumentally meteorological records and historical dryness/wetness indexes from the studying area, indicating that stalagmite δ18O on annual-to-decadal scales reflects mainly rainfall change in the monsoonal area, with lighter δ18O reflecting wetter condition. The comparison of the δ18O with the East Asian Summer Monsoon (EASM) index since AD 1850 adopted by IPCC (2007) illustrates that lighter stalagmite δ18O corresponds to stronger EASM. Taking the average δ18O value of the 1000-yr record as an average climatic condition, we have found that the δ18O values during AD 1050~1100, 1125~1225, 1300~1350, 1430~1630 and 1900~1960 are lighter than the average, reflecting stronger EASM and wetter condition. The δ18O values during AD 1100~1125, 1225~1300, 1350~1430, 1630~1900 and 1960 to the present are heavier than the average, indicating weak EASM and drier condition. On decadal-to-centennial scales, the δ13C variations generally follow that of δ18O which probably demonstrates that vegetation above the cave controls the δ13C variations. Further comparisons of the δ18O, EASM, ENSO and PDO show that a negative SOI (El Nino phase) correlates warm PDO phase and higher N. Pacific pressure, resulting

21 CFR 500.46 - Hexachlorophene in animal drugs.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Hexachlorophene in animal drugs. 500.46 Section 500.46 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS GENERAL Specific Administrative Rulings and Decisions § 500.46...

9 CFR 381.500 - Exemption from nutrition labeling.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Exemption from nutrition labeling. 381.500 Section 381.500 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF... business is any single-plant facility or multi-plant company/firm that employs 500 or fewer people and...

Satellite myths

Science.gov (United States)

Easton, Roger L.; Hall, David

2008-01-01

Richard Corfield's article “Sputnik's legacy” (October 2007 pp23-27) states that the satellite on board the US Vanguard rocket, which exploded during launch on 6 December 1957 two months after Sputnik's successful take-off, was “a hastily put together contraption of wires and circuitry designed only to send a radio signal back to Earth”. In fact, the Vanguard satellite was developed over a period of several years and put together carefully using the best techniques and equipment available at the time - such as transistors from Bell Laboratories/Western Electric. The satellite contained not one but two transmitters, in which the crystal-controlled oscillators had been designed to measure both the temperature of the satellite shell and of the internal package.

Communication satellite applications

Science.gov (United States)

Pelton, Joseph N.

The status and future of the technologies, numbers and services provided by communications satellites worldwide are explored. The evolution of Intelsat satellites and the associated earth terminals toward high-rate all-digital telephony, data, facsimile, videophone, videoconferencing and DBS capabilities are described. The capabilities, services and usage of the Intersputnik, Eutelsat, Arabsat and Palapa systems are also outlined. Domestic satellite communications by means of the Molniya, ANIK, Olympus, Intelsat and Palapa spacecraft are outlined, noting the fast growth of the market and the growing number of different satellite manufacturers. The technical, economic and service definition issues surrounding DBS systems are discussed, along with presently operating and planned maritime and aeronautical communications and positioning systems. Features of search and rescue and tracking, data, and relay satellite systems are summarized, and services offered or which will be offered by every existing or planned communication satellite worldwide are tabulated.

500 French verbs for dummies

CERN Document Server

Erotopoulos

2013-01-01

Vexed by French verbs? Fear no more! In 500 French Verbs For Dummies, beginning French language learners can find a quick reference for verbs in the basic present tenses. More advanced French speakers can utilize this book to learn more complex verb tenses and conjugations as well as advanced verbs with irregular endings. One page for each of the 500 most commonly used verbs in the French language -alphabetically arranged and numbered for easy referenceSpecial designation of the 50 most essential French verbsA summary of basic French grammar that incl

29 CFR 500.143 - Civil money penalty assessment.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Civil money penalty assessment. 500.143 Section 500.143... MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Enforcement § 500.143 Civil money penalty assessment. (a) A civil money penalty may be assessed for each violation of the Act or these regulations. (b) In...

Fortune 500 Corporate Headquarters

Data.gov (United States)

Department of Homeland Security — Large Corporate Headquarters in the United States This database is composed of 'an annual list of the 500 largest industrial corporations in the U.S., published by...

Geant4-DNA: overview and recent developments

Science.gov (United States)

Štěpán, Václav

Space travel and high altitude flights are inherently associated with prolonged exposure to cosmic and solar radiation. Understanding and simulation of radiation action on cellular and subcellular level contributes to precise assessment of the associated health risks and remains a challenge of today’s radiobiology research. The Geant4-DNA project (http://geant4-dna.org) aims at developing an experimentally validated simulation platform for modelling of the damage induced by ionizing radiation at DNA level. The platform is based on the Geant4 Monte Carlo simulation toolkit. This project extends specific functionalities of Geant4 in following areas: The step-by-step single scattering modelling of elementary physical interactions of electrons, protons, alpha particles and light ions with liquid water and DNA bases, for the so-called “physical” stage. The modelling of the “physico-chemical and chemical” stages corresponding to the production, the diffusion, the chemical reactions occurring between chemical species produced by water radiolysis, and to the radical attack on the biological targets. Physical and chemical stage simulations are combined with biological target models on several scales, from DNA double helix, through nucleosome, to chromatin segments and cell geometries. In addition, data mining clustering algorithms have been developed and optimised for the purpose of DNA damage scoring in simulated tracks. Experimental measurements on pBR322 plasmid DNA are being carried out in order to validate the Geant4-DNA models. The plasmid DNA has been irradiated in dry conditions by protons with energies from 100 keV to 30 MeV and in aqueous conditions, with and without scavengers, by 30 MeV protons, 290 MeV/u carbon and 500 MeV/u iron ions. Agarose gel electrophoresis combined with enzymatic treatment has been used to measure the resulting DNA damage. An overview of the developments undertaken by the Geant4-DNA collaboration including a description of

IAEA Safeguards: Cost/benefit analysis of commercial satellite imagery

Energy Technology Data Exchange (ETDEWEB)

Andersson, Christer [SSC Satellitbild AB, Kiruna (Sweden)

1999-03-01

feasible undertaking. Such a unit capable of performing advanced image processing as a tool for various safeguard tasks will give the Agency an effective instrument for reference, monitoring, verification, and detection of declared and undeclared activities. It is also demonstrated in the study that more than 20% of the envisaged work tasks in all of the major Safeguards applications will achieve a better decision support from the use of commercial satellite imagery. At the same time the potential savings in costs is calculated to approximately USD 500,000 per year by reductions in on-site inspections and by more efficient planning and logistics. The total cost for implementing commercial satellite imagery at the Department for Safeguards, as simulated in this study, is approximately USD 1,500,000 per year. This cost rises when utilising the full potential of high-resolution imagery in all five applications including monitoring and detection of undeclared facilities. The cost/benefit simulation is founded on an activity scenario with a staff of 4 experts working in an IAEA imagery unit with a workload of three dossiers or `issues` per week. The imagery unit is built around an advanced workstation PC image processing system capable of handling several hundreds of pre-processed imagery per year 10 refs, 9 figs, 5 tabs

Melanesian mtDNA complexity.

Directory of Open Access Journals (Sweden)

Jonathan S Friedlaender

Full Text Available Melanesian populations are known for their diversity, but it has been hard to grasp the pattern of the variation or its underlying dynamic. Using 1,223 mitochondrial DNA (mtDNA sequences from hypervariable regions 1 and 2 (HVR1 and HVR2 from 32 populations, we found the among-group variation is structured by island, island size, and also by language affiliation. The more isolated inland Papuan-speaking groups on the largest islands have the greatest distinctions, while shore dwelling populations are considerably less diverse (at the same time, within-group haplotype diversity is less in the most isolated groups. Persistent differences between shore and inland groups in effective population sizes and marital migration rates probably cause these differences. We also add 16 whole sequences to the Melanesian mtDNA phylogenies. We identify the likely origins of a number of the haplogroups and ancient branches in specific islands, point to some ancient mtDNA connections between Near Oceania and Australia, and show additional Holocene connections between Island Southeast Asia/Taiwan and Island Melanesia with branches of haplogroup E. Coalescence estimates based on synonymous transitions in the coding region suggest an initial settlement and expansion in the region at approximately 30-50,000 years before present (YBP, and a second important expansion from Island Southeast Asia/Taiwan during the interval approximately 3,500-8,000 YBP. However, there are some important variance components in molecular dating that have been overlooked, and the specific nature of ancestral (maternal Austronesian influence in this region remains unresolved.

Multilocus DNA fingerprints in gallinaceous birds: general approach and problems.

Science.gov (United States)

Hanotte, O; Bruford, M W; Burke, T

1992-06-01

Multilocus profiles were investigated in five different species of Galliformes (ring-necked pheasant Phasianus colchicus, Indian peafowl Pavo cristatus, Japanese quail Coturnix coturnix japonica, domestic chicken Gallus gallus, and red grouse Lagopus lagopus scoticus) using two human multilocus probes (33.6 and 33.15) in combination with each of four restriction enzymes (AluI, DdeI, HaeIII or HinfI). All the species show a DNA fingerprint-like pattern using at least one restriction enzyme in combination with each multilocus probe. The number of bands detected and the value of the index of similarity for each species differ significantly between the profiles obtained with each multilocus probe. Some enzyme/probe combinations reveal strong cross-hybridization of the multilocus probes with satellite or satellite-like DNA sequences in pheasant, peacock, quail and chicken, which partially or completely prevented scoring of the profile. The choice of restriction enzyme was found to influence the number of bands, the value of the index of similarity and the probability of obtaining an identical fingerprint between unrelated individuals. The Mendelian inheritance and independent segregation of the fragments detected using AluI was investigated in three species (ring-necked pheasant, Indian peafowl and red grouse). Some bands were shown to be tightly linked. An extreme case was encountered in the red grouse, where 12 of the 15 bands scored in one parent represented only two, apparently allelic, haplotypes and so derived from a single locus. However, fingerprint patterns will often be adequate for use in paternity analyses, such as in behavioural studies, despite the occurrence of haplotypic sets of bands. Identical DNA multilocus profiles were sometimes observed between captive-bred siblings in one species. These results emphasize the desirability of determining, in each new species, the optimal experimental conditions as a preliminary to any behavioural or population

78 FR 11089 - Special Conditions: Bombardier Aerospace, Model BD-500-1A10 and BD-500-1A11 Airplanes; Sidestick...

Science.gov (United States)

2013-02-15

...; Special Conditions No. 25-477-SC] Special Conditions: Bombardier Aerospace, Model BD-500-1A10 and BD-500... special conditions. SUMMARY: These special conditions are issued for the Bombardier Aerospace Model BD-500...: Background On December 10, 2009, Bombardier Aerospace applied for a type certificate for their new Model BD...

History of Satellite TV Broadcasting and Satellite Broadcasting Market in Turkey

Directory of Open Access Journals (Sweden)

Mihalis KUYUCU

2015-09-01

Full Text Available The present study analyses the satellite broadcasting that is the first important development that emerged as a result of digitalization in communication technologies and its reflections in Turkey. As the first milestone in the globalization of television broadcasting, satellite broadcasting provided substantial contribution towards the development of the media. Satellite bro adcasting both increased the broadcasting quality and geographical coverage of the television media. A conceptual study was carried out in the first part of the study in connection with the history of satellite broadcasting in Turkey and across the world. In the research part of the study, an analysis was performed on 160 television channels that broadcast in Turkey via Turksat Satellite. Economic structure of the television channels broadcasting in Turkey via satellite was studied and an analysis was perfo rmed on the operational structure of the channels. As a result of the study, it was emphasized that the television channels broadcasting via satellite platform also use other platforms for the purpose of spreading their broadcasts and television channel ow ners make investments in different branches of the media, too. Capital owners invest in different business areas other than the media although television channels broadcasting via Turksat mostly focus on thematic broadcasting and make effort to generate ec onomic income from advertisements. Delays are encountered in the course of the convergence between the new media and television channels that broadcast only from the satellite platform and such television channels experience more economic problems than the other channels. New media and many TV broadcasting platforms emerged as a result of the developments in the communication technologies. In television broadcasting, satellite platform is not an effective platform on its own. Channels make effort to reach t o more people by using other platforms in addition to

Study in regularities in the formation of double stranded DNA breaks in irradiated rat thymocytes

International Nuclear Information System (INIS)

Ivannik, B.P.; ProskuryakoV, S.Ya.; Ryabchenko, N.I.

1979-01-01

Using low-gradient viscosimetry of neutral detergent nuclear lysates a study was made of postradiation changes in the molecular weight of double-stranded DNA of thymocytes. It was established that 375 eV are needed for one double-stranded break to appear, and a dose of 1 rad is required for 0.275 double-stranded break to occur at the site of DNA with m.w. 10 12 dalton. The repair of double-stranded breaks is only observed when rats are exposed to a dose of 500 R. It is assumed that the absence of repair of double-stranded DNA breaks and the presence of secondary postradiation degradation of DNA are responsible for thymocyte death

Relative tracking control of constellation satellites considering inter-satellite link

Science.gov (United States)

Fakoor, M.; Amozegary, F.; Bakhtiari, M.; Daneshjou, K.

2017-11-01

In this article, two main issues related to the large-scale relative motion of satellites in the constellation are investigated to establish the Inter Satellite Link (ISL) which means the dynamic and control problems. In the section related to dynamic problems, a detailed and effective analytical solution is initially provided for the problem of satellite relative motion considering perturbations. The direct geometric method utilizing spherical coordinates is employed to achieve this solution. The evaluation of simulation shows that the solution obtained from the geometric method calculates the relative motion of the satellite with high accuracy. Thus, the proposed analytical solution will be applicable and effective. In the section related to control problems, the relative tracking control system between two satellites will be designed in order to establish a communication link between the satellites utilizing analytical solution for relative motion of satellites with respect to the reference trajectory. Sliding mode control approach is employed to develop the relative tracking control system for body to body and payload to payload tracking control. Efficiency of sliding mode control approach is compared with PID and LQR controllers. Two types of payload to payload tracking control considering with and without payload degree of freedom are designed and suitable one for practical ISL applications is introduced. Also, Fuzzy controller is utilized to eliminate the control input in the sliding mode controller.

Mobile satellite service communications tests using a NASA satellite

Science.gov (United States)

Chambers, Katherine H.; Koschmeder, Louis A.; Hollansworth, James E.; ONeill, Jack; Jones, Robert E.; Gibbons, Richard C.

1995-01-01

Emerging applications of commercial mobile satellite communications include satellite delivery of compact disc (CD) quality radio to car drivers who can select their favorite programming as they drive any distance; transmission of current air traffic data to aircraft; and handheld communication of data and images from any remote corner of the world. Experiments with the enabling technologies and tests and demonstrations of these concepts are being conducted before the first satellite is launched by utilizing an existing NASA spacecraft.

Study on electron fluxes with Esub(e)=40-500 keV in quiet periods of solar activity by means of correlation and spectral analysis technique

International Nuclear Information System (INIS)

Zel'dovich, M.A.; Trebukhovskaya, G.A.

1982-01-01

Background fluxes of low-energy electrons (Esub(e)=40-500 keV), observed in the interplanetary space in the absence of solar cosmic ray flares using the ''Prognoz-3'' artificial satellite during 1973-1974, are under study. Fluctuation power spectra and correlation functions of simultaneous series of fluxes of above electrons, galactic cosmic rays are calculated along with some parameters characterizing solar activity and interplanetary medium state. The mentioned analysis points to possible solar origin of background low-energy electron fluxes

Satellite Geomagnetism

DEFF Research Database (Denmark)

Olsen, Nils; Stolle, Claudia

2012-01-01

Observations of Earth’s magnetic field from space began more than 50 years ago. A continuous monitoring of the field using low Earth orbit (LEO) satellites, however, started only in 1999, and three satellites have taken highprecision measurements of the geomagnetic field during the past decade....... The unprecedented time-space coverage of their data opened revolutionary new possibilities for monitoring, understanding, and exploring Earth’s magnetic field. In the near future, the three-satellite constellation Swarm will ensure continuity of such measurement and provide enhanced possibilities to improve our...... ability to characterize and understand the many sources that contribute to Earth’s magnetic field. In this review, we summarize investigations of Earth’s interior and environment that have been possible through the analysis of high-precision magnetic field observations taken by LEO satellites....

The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition.

Science.gov (United States)

Fulka, Helena; Langerova, Alena

2014-04-01

The oocyte (maternal) nucleolus is essential for early embryonic development and embryos originating from enucleolated oocytes arrest at the 2-cell stage. The reason for this is unclear. Surprisingly, RNA polymerase I activity in nucleolus-less mouse embryos, as manifested by pre-rRNA synthesis, and pre-rRNA processing are not affected, indicating an unusual role of the nucleolus. We report here that the maternal nucleolus is indispensable for the regulation of major and minor satellite repeats soon after fertilisation. During the first embryonic cell cycle, absence of the nucleolus causes a significant reduction in major and minor satellite DNA by 12% and 18%, respectively. The expression of satellite transcripts is also affected, being reduced by more than half. Moreover, extensive chromosome bridging of the major and minor satellite sequences was observed during the first mitosis. Finally, we show that the absence of the maternal nucleolus alters S-phase dynamics and causes abnormal deposition of the H3.3 histone chaperone DAXX in pronuclei of nucleolus-less zygotes.

Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation

Directory of Open Access Journals (Sweden)

Simonetta Geninatti Crich

2005-01-01

Full Text Available The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, Ka = 2.2 × 103 M−1 for approximately 2500 ± 500 binding sites. Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.

Interim Service ISDN Satellite (ISIS) simulator development for advanced satellite designs and experiments

Science.gov (United States)

Pepin, Gerard R.

1992-01-01

The simulation development associated with the network models of both the Interim Service Integrated Services Digital Network (ISDN) Satellite (ISIS) and the Full Service ISDN Satellite (FSIS) architectures is documented. The ISIS Network Model design represents satellite systems like the Advanced Communications Technology Satellite (ACTS) orbiting switch. The FSIS architecture, the ultimate aim of this element of the Satellite Communications Applications Research (SCAR) Program, moves all control and switching functions on-board the next generation ISDN communications satellite. The technical and operational parameters for the advanced ISDN communications satellite design will be obtained from the simulation of ISIS and FSIS engineering software models for their major subsystems. Discrete event simulation experiments will be performed with these models using various traffic scenarios, design parameters, and operational procedures. The data from these simulations will be used to determine the engineering parameters for the advanced ISDN communications satellite.

The phytochemical 3,3'-diindolylmethane decreases expression of AR-controlled DNA damage repair genes through repressive chromatin modifications and is associated with DNA damage in prostate cancer cells.

Science.gov (United States)

Palomera-Sanchez, Zoraya; Watson, Gregory W; Wong, Carmen P; Beaver, Laura M; Williams, David E; Dashwood, Roderick H; Ho, Emily

2017-09-01

Androgen receptor (AR) is a transcription factor involved in normal prostate physiology and prostate cancer (PCa) development. 3,3'-Diindolylmethane (DIM) is a promising phytochemical agent against PCa that affects AR activity and epigenetic regulators in PCa cells. However, whether DIM suppresses PCa via epigenetic regulation of AR target genes is unknown. We assessed epigenetic regulation of AR target genes in LNCaP PCa cells and showed that DIM treatment led to epigenetic suppression of AR target genes involved in DNA repair (PARP1, MRE11, DNA-PK). Decreased expression of these genes was accompanied by an increase in repressive chromatin marks, loss of AR occupancy and EZH2 recruitment to their regulatory regions. Decreased DNA repair gene expression was associated with an increase in DNA damage (γH2Ax) and up-regulation of genomic repeat elements LINE1 and α-satellite. Our results suggest that DIM suppresses AR-dependent gene transcription through epigenetic modulation, leading to DNA damage and genome instability in PCa cells. Published by Elsevier Inc.

DNA methylation at a bovine alpha satellite I repeat CpG site during development following fertilization and somatic cell nuclear transfer.

Directory of Open Access Journals (Sweden)

Christine Couldrey

Full Text Available Incomplete epigenetic reprogramming is postulated to contribute to the low developmental success following somatic cell nuclear transfer (SCNT. Here, we describe the epigenetic reprogramming of DNA methylation at an alpha satellite I CpG site (αsatI-5 during development of cattle generated either by artificial insemination (AI or in vitro fertilization (IVF and SCNT. Quantitative methylation analysis identified that SCNT donor cells were highly methylated at αsatI-5 and resulting SCNT blastocysts showed significantly more methylation than IVF blastocysts. At implantation, no difference in methylation was observed between SCNT and AI in trophoblast tissue at αsatI-5, however, SCNT embryos were significantly hyper-methylated compared to AI controls at this time point. Following implantation, DNA methylation at αsatI-5 decreased in AI but not SCNT placental tissues. In contrast to placenta, the proportion of methylation at αsatI-5 remained high in adrenal, kidney and muscle tissues during development. Differences in the average proportion of methylation were smaller in somatic tissues than placental tissues but, on average, SCNT somatic tissues were hyper-methylated at αsatI-5. Although sperm from all bulls was less methylated than somatic tissues at αsatI-5, on average this site remained hyper-methylated in sperm from cloned bulls compared with control bulls. This developmental time course confirms that epigenetic reprogramming does occur, at least to some extent, following SCNT. However, the elevated methylation levels observed in SCNT blastocysts and cellular derivatives implies that there is either insufficient time or abundance of appropriate reprogramming factors in oocytes to ensure complete reprogramming. Incomplete reprogramming at this CpG site may be a contributing factor to low SCNT success rates, but more likely represents the tip of the iceberg in terms of incompletely reprogramming. Until protocols ensure the epigenetic

[Study on three kinds of gasoline oxygenates-induced DNA damage in mice fibroblasts].

Science.gov (United States)

Song, Chonglin; Zhang, Zhifu; Chen, Xue; Zhang, Yanfeng; Wang, Chunhua; Liu, Keming

2002-10-01

To study DNA damage of three kinds of gasoline oxygenates. Single cell gel electrophoresis assay(Comet assay) was used to detect the damage effects of three gasoline oxygenates[methyl tertiary butyl ether(MTBE), ethanol anhydrous(EA) and dimethyl carbonate(DMC)] on DNA in L-929 mice fibroblasts. In certain concentation(37.500-150.000 mg/ml), MTBE could directly cause DNA damage of L-929 mice fibroblasts. There was obvious dose-effect relationship, i.e. when the concentration of MTBE was increased from 9.375 to 150.000 mg/ml, the comet rate also increased from 4% to 85%, and the length of comet tail changed correspondingly. The results of EA and DMC were negative. Under the condition of this experiment(150.000 mg/ml), MTBE could directly cause DNA damage while the effect of EA and DMC on DNA damage was not found.

500 Cities: City Boundaries

Data.gov (United States)

U.S. Department of Health & Human Services — This city boundary shapefile was extracted from Esri Data and Maps for ArcGIS 2014 - U.S. Populated Place Areas. This shapefile can be joined to 500 Cities...

29 CFR 780.305 - 500 man-day provision.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false 500 man-day provision. 780.305 Section 780.305 Labor...) Statutory Provisions § 780.305 500 man-day provision. (a) Section 3(u) of the Act defines man-day to mean “any day during which an employee performs agricultural labor for not less than 1 hour.” 500 man-days...

Handbook of satellite applications

CERN Document Server

Madry, Scott; Camacho-Lara, Sergio

2017-01-01

The first edition of this ground breaking reference work was the most comprehensive reference source available about the key aspects of the satellite applications field. This updated second edition covers the technology, the markets, applications and regulations related to satellite telecommunications, broadcasting and networking—including civilian and military systems; precise satellite navigation and timing networks (i.e. GPS and others); remote sensing and meteorological satellite systems. Created under the auspices of the International Space University based in France, this brand new edition is now expanded to cover new innovative small satellite constellations, new commercial launching systems, innovation in military application satellites and their acquisition, updated appendices, a useful glossary and more.

Satellite image collection optimization

Science.gov (United States)

Martin, William

2002-09-01

Imaging satellite systems represent a high capital cost. Optimizing the collection of images is critical for both satisfying customer orders and building a sustainable satellite operations business. We describe the functions of an operational, multivariable, time dynamic optimization system that maximizes the daily collection of satellite images. A graphical user interface allows the operator to quickly see the results of what if adjustments to an image collection plan. Used for both long range planning and daily collection scheduling of Space Imaging's IKONOS satellite, the satellite control and tasking (SCT) software allows collection commands to be altered up to 10 min before upload to the satellite.

Trends in communications satellites

CERN Document Server

Curtin, Denis J

1979-01-01

Trends in Communications Satellites offers a comprehensive look at trends and advances in satellite communications, including experimental ones such as NASA satellites and those jointly developed by France and Germany. The economic aspects of communications satellites are also examined. This book consists of 16 chapters and begins with a discussion on the fundamentals of electrical communications and their application to space communications, including spacecraft, earth stations, and orbit and wavelength utilization. The next section demonstrates how successful commercial satellite communicati

Theory of satellite geodesy applications of satellites to geodesy

CERN Document Server

Kaula, William M

2000-01-01

The main purpose of this classic text is to demonstrate how Newtonian gravitational theory and Euclidean geometry can be used and developed in the earth's environment. The second is to collect and explain some of the mathematical techniques developed for measuring the earth by satellite.Book chapters include discussions of the earth's gravitational field, with special emphasis on spherical harmonies and the potential of the ellipsoid; matrices and orbital geometry; elliptic motion, linear perturbations, resonance, and other aspects of satellite orbit dynamics; the geometry of satellite obser

Sea level reconstruction: Exploration of methods for combining altimetry with other data to beyond the 20-year altimetric record

DEFF Research Database (Denmark)

Svendsen, Peter Limkilde; Andersen, Ole Baltazar; Nielsen, Allan Aasbjerg

2012-01-01

Ocean satellite altimetry has provided global sets of sea level data for the last two decades, allowing determination of spatial patterns in global sea level. For reconstructions going back further than this period, tide gauge data can be used as a proxy for the model. We examine different methods...... to spatial distribution, and tide gauge data are available around the Arctic Ocean, which may be important for a later high-latitude reconstruction....... of combining satellite altimetry and tide gauge data using optimal weighting of tide gauge data, linear regression and EOFs, including automatic quality checks of the tide gauge time series. We attempt to augment the model using various proxies such as climate indices like the NAO and PDO, and investigate...

Thermospheric density and satellite drag modeling

Science.gov (United States)

Mehta, Piyush Mukesh

GRACE satellites. Moving toward accurate atmospheric models and absolute densities requires physics based models for CD. Closed-form solutions of CD have been developed and exist for a handful of simple geometries (flat plate, sphere, and cylinder). However, for complex geometries, the Direct Simulation Monte Carlo (DSMC) method is an important tool for developing CD models. DSMC is computationally intensive and real-time simulations for CD are not feasible. Therefore, parameterized models for CD are required. Modeling CD for an RSO requires knowledge of the gas-surface interaction (GSI) that defines the manner in which the atmospheric particles exchange momentum and energy with the surface. The momentum and energy exchange is further influenced by likely adsorption of atomic oxygen that may partially or completely cover the surface. An important parameter that characterizes the GSI is the energy accommodation coefficient, α. An innovative and state-of-the-art technique of developing parameterized drag coefficient models is presented and validated using the GRACE satellite. The effect of gas-surface interactions on physical drag coefficients is examined. An attempt to reveal the nature of gas-surface interactions at altitudes above 500 km is made using the STELLA satellite. A model that can accurately estimate CD has the potential to: (i) reduce the sources of uncertainty in the drag model, (ii) improve density estimates by resolving time-varying biases and moving toward absolute densities, and (iii) increase data sources for density estimation by allowing for the use of a wide range of RSOs as information sources. Results from this work have the potential to significantly improve the accuracy of conjunction analysis and SSA.

Non-random alkylation of DNA sequences induced in vivo by chemical mutagens

Energy Technology Data Exchange (ETDEWEB)

Durante, M.; Geri, C.; Bonatti, S.; Parenti, R. (Universita di Pisa (Italy))

1989-08-01

Previous studies of the interaction of alkylating agents on the eukaryotic genome support the idea that induction of DNA adducts is at specific genomic sites. Here we show molecular and cytological evidence that alkylation is rather specific. Mammalian cell cultures were exposed to different doses of mutagens and the DNA was analyzed by density gradient ultracentrifugation, hydroxylapatite fractionation, and by restriction enzyme analysis. Studies with the labelled mutagens N-ethyl-N-nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine show that there is a non-random distribution of the adducts. The adducts are found more frequently in A-T, G-C rich satellite DNA and highly repetitive sequences. Analysis with restriction enzymes shows that both methyl and ethyl groups influence the restriction patterns of the enzymes HpaII and MspI that recognize specific endogenous DNA methylation. These data suggest, as a subsequent mechanism, a modification in the pattern of the normal endogenous methylation of 5-methylcytosine.

31 CFR 500.572 - Humanitarian projects authorized.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Humanitarian projects authorized. 500.572 Section 500.572 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... from private sources, including but not limited to accredited degree-granting institutes of education...

13 CFR 146.500 - Secretary of Defense.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Secretary of Defense. 146.500 Section 146.500 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW RESTRICTIONS ON LOBBYING..., a covered Federal action from the prohibition whenever the Secretary determines, in writing, that...

40 CFR 152.500 - Requirements for devices.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Requirements for devices. 152.500 Section 152.500 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS... other than a bacterium, virus, or other microorganism on or in living man or living animals) but not...

Earth Radiation Imbalance from a Constellation of 66 Iridium Satellites: Technological Aspects

Science.gov (United States)

Wiscombe, W.; Chiu, C. J-Y.

2012-01-01

Iridium Communications Inc. is launching a new generation of polar orbiting communication satellites in 2015-2017. Iridium will provide a hosted payload bay on each of the 66 satellites (plus 6 in-space spares). This offers the potential for a paradigm shift in the way we measure Earth radiation imbalance from space, as well as massive cost savings. Because the constellation provides 24/7 global coverage, there is no need to account for diurnal cycle via extrapolations from uncalibrated narrowband geostationary imagers. And the spares can be rolled over to view the Sun and deep space, then transfer their calibration to the other members of the constellation during the frequent cross-overs. In part using simulations of the constellation viewing realistic Earth scenes, this presentation will address the technological aspects of such a constellation: (1) the calibration strategy; (2) the highly-accurate and stable radiometers for measuring outgoing flux; and (3) the GRACE-inspired algorithms for representing the outgoing flux field in spherical harmonics and thus achieving rv500-km spatial resolution and two-hour temporal resolution.

Variability in Surface BRDF at Different Spatial Scales (30m-500m) Over a Mixed Agricultural Landscape as Retrieved from Airborne and Satellite Spectral Measurements

Science.gov (United States)

Roman, Miguel O.; Gatebe, Charles K.; Schaaf, Crystal B.; Poudyal, Rajesh; Wang, Zhuosen; King, Michael D.

2012-01-01

Over the past decade, the role of multiangle 1 remote sensing has been central to the development of algorithms for the retrieval of global land surface properties including models of the bidirectional reflectance distribution function (BRDF), albedo, land cover/dynamics, burned area extent, as well as other key surface biophysical quantities represented by the anisotropic reflectance characteristics of vegetation. In this study, a new retrieval strategy for fine-to-moderate resolution multiangle observations was developed, based on the operational sequence used to retrieve the Moderate Resolution Imaging Spectroradiometer (MODIS) Collection 5 reflectance and BRDF/albedo products. The algorithm makes use of a semiempirical kernel-driven bidirectional reflectance model to provide estimates of intrinsic albedo (i.e., directional-hemispherical reflectance and bihemispherical reflectance), model parameters describing the BRDF, and extensive quality assurance information. The new retrieval strategy was applied to NASA's Cloud Absorption Radiometer (CAR) data acquired during the 2007 Cloud and Land Surface Interaction Campaign (CLASIC) over the well-instrumented Atmospheric Radiation Measurement Program (ARM) Southern Great Plains (SGP) Cloud and Radiation Testbed (CART) site in Oklahoma, USA. For the case analyzed, we obtained approx.1.6 million individual surface bidirectional reflectance factor (BRF) retrievals, from nadir to 75deg off-nadir, and at spatial resolutions ranging from 3 m - 500 m. This unique dataset was used to examine the interaction of the spatial and angular 18 characteristics of a mixed agricultural landscape; and provided the basis for detailed assessments of: (1) the use of a priori knowledge in kernel-driven BRDF model inversions; (2) the interaction between surface reflectance anisotropy and instrument spatial resolution; and (3) the uncertainties that arise when sub-pixel differences in the BRDF are aggregated to a moderate resolution satellite

In vivo control of CpG and non-CpG DNA methylation by DNA methyltransferases.

Directory of Open Access Journals (Sweden)

Julia Arand

2012-06-01

Full Text Available The enzymatic control of the setting and maintenance of symmetric and non-symmetric DNA methylation patterns in a particular genome context is not well understood. Here, we describe a comprehensive analysis of DNA methylation patterns generated by high resolution sequencing of hairpin-bisulfite amplicons of selected single copy genes and repetitive elements (LINE1, B1, IAP-LTR-retrotransposons, and major satellites. The analysis unambiguously identifies a substantial amount of regional incomplete methylation maintenance, i.e. hemimethylated CpG positions, with variant degrees among cell types. Moreover, non-CpG cytosine methylation is confined to ESCs and exclusively catalysed by Dnmt3a and Dnmt3b. This sequence position-, cell type-, and region-dependent non-CpG methylation is strongly linked to neighboring CpG methylation and requires the presence of Dnmt3L. The generation of a comprehensive data set of 146,000 CpG dyads was used to apply and develop parameter estimated hidden Markov models (HMM to calculate the relative contribution of DNA methyltransferases (Dnmts for de novo and maintenance DNA methylation. The comparative modelling included wild-type ESCs and mutant ESCs deficient for Dnmt1, Dnmt3a, Dnmt3b, or Dnmt3a/3b, respectively. The HMM analysis identifies a considerable de novo methylation activity for Dnmt1 at certain repetitive elements and single copy sequences. Dnmt3a and Dnmt3b contribute de novo function. However, both enzymes are also essential to maintain symmetrical CpG methylation at distinct repetitive and single copy sequences in ESCs.

Satellite Communications

Indian Academy of Sciences (India)

First page Back Continue Last page Overview Graphics. Satellite Communications. Arthur C Clarke wrote a seminal paper in 1945 in wireless world. Use three satellites in geo-synchronous orbit to enable intercontinental communications. System could be realised in '50 to 100 years'

Culture-Negative Endocarditis Diagnosed Using 16S DNA Polymerase Chain Reaction

Directory of Open Access Journals (Sweden)

Stephen Duffett

2012-01-01

Full Text Available 16S DNA polymerase chain reaction (PCR is a molecular amplification technique that can be used to identify bacterial pathogens in culture-negative endocarditis. Bacterial DNA can be isolated from surgically excised valve tissue or from blood collected in EDTA vials. Use of this technique is particularly helpful in identifying the bacterial pathogen in cases of culture-negative endocarditis. A case involving a 48-year-old man who presented with severe aortic regurgitation and a four-month prodrome of low-grade fever is reported. Blood and valve tissue cultures following valve replacement were negative. A valve tissue sample was sent for investigation with 16S DNA PCR, which successfully identified Streptococcus salivarius and was interpreted as the true diagnosis. A review of the literature suggests that 16S DNA PCR from valve tissue is a more sensitive diagnostic test than culture. It is also extremely specific, based on a sequence match of at least 500 base pairs.

Cell-associated HIV DNA measured early during infection has prognostic value independent of serum HIV RNA measured concomitantly

DEFF Research Database (Denmark)

Katzenstein, Terese L; Oliveri, Roberto S; Benfield, Thomas

2002-01-01

Using data from the Danish AIDS Cohort of HIV-infected homosexual men established in the 1980s, the prognostic value of early HIV DNA loads was evaluated. In addition to DNA measurements, concomitant serum HIV RNA levels, CD4 cell counts and CCR5 genotypes were determined. The patients were divided...... into 3 groups, according to whether their cell-associated HIV DNA load was or = 2,500 DNA copies/10(6) peripheral blood mononuclear cells. Clinical progression rates differed significantly between the groups (p value independent...... of serum HIV RNA (p value. Patients heterozygous for the CCR5 delta 32 allele had significantly lower HIV DNA loads than those homozygous for the normal allele (p

Assessment of ASSERT-PV for prediction of post-dryout heat transfer in CANDU bundles

International Nuclear Information System (INIS)

Cheng, Z.; Rao, Y.F.; Waddington, G.M.

2014-01-01

Highlights: • Assessment of the new Canadian subchannel code ASSERT-PV 3.2 for PDO sheath temperature prediction. • CANDU 28-, 37- and 43-element bundle PDO experiments. • Prediction improvement of ASSERT-PV 3.2 over previous code versions. • Sensitivity study of the effect of PDO model options. - Abstract: Atomic Energy of Canada Limited (AECL) has developed the subchannel thermalhydraulics code ASSERT-PV for the Canadian nuclear industry. The recently released ASSERT-PV 3.2 provides enhanced models for improved predictions of subchannel flow distribution, critical heat flux (CHF), and post-dryout (PDO) heat transfer in horizontal CANDU fuel channels. This paper presents results of an assessment of the new code version against PDO tests performed during five full-size CANDU bundle experiments conducted between 1992 and 2009 by Stern Laboratories (SL), using 28-, 37- and 43-element bundles. A total of 10 PDO test series with varying pressure-tube creep and/or bearing-pad height were analyzed. The SL experiments encompassed the bundle geometries and range of flow conditions for the intended ASSERT-PV applications for existing CANDU reactors. Code predictions of maximum PDO fuel-sheath temperature were compared against measurements from the SL PDO tests to quantify the code's prediction accuracy. The prediction statistics using the recommended model set of ASSERT-PV 3.2 were compared to those from previous code versions. Furthermore, separate-effects sensitivity studies quantified the contribution of each PDO model change or enhancement to the improvement in PDO heat transfer prediction. Overall, the assessment demonstrated significant improvement in prediction of PDO sheath temperature in horizontal fuel channels containing CANDU bundles

24 CFR 954.500 - Repayment of investment.

Science.gov (United States)

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Repayment of investment. 954.500... DEVELOPMENT INDIAN HOME PROGRAM Program Administration § 954.500 Repayment of investment. (a) HOME funds will be made available pursuant to a HOME Investment Partnership Agreement. The agreement ensures that...

31 CFR 500.325 - National securities exchange.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false National securities exchange. 500.325... Definitions § 500.325 National securities exchange. The term national securities exchange shall mean an exchange registered as a national securities exchange under section 6 of the Securities Exchange Act of...

Optimization of DNA Sensor Model Based Nanostructured Graphene Using Particle Swarm Optimization Technique

Directory of Open Access Journals (Sweden)

Hediyeh Karimi

2013-01-01

Full Text Available It has been predicted that the nanomaterials of graphene will be among the candidate materials for postsilicon electronics due to their astonishing properties such as high carrier mobility, thermal conductivity, and biocompatibility. Graphene is a semimetal zero gap nanomaterial with demonstrated ability to be employed as an excellent candidate for DNA sensing. Graphene-based DNA sensors have been used to detect the DNA adsorption to examine a DNA concentration in an analyte solution. In particular, there is an essential need for developing the cost-effective DNA sensors holding the fact that it is suitable for the diagnosis of genetic or pathogenic diseases. In this paper, particle swarm optimization technique is employed to optimize the analytical model of a graphene-based DNA sensor which is used for electrical detection of DNA molecules. The results are reported for 5 different concentrations, covering a range from 0.01 nM to 500 nM. The comparison of the optimized model with the experimental data shows an accuracy of more than 95% which verifies that the optimized model is reliable for being used in any application of the graphene-based DNA sensor.

Geostationary Satellite (GOES) Images

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Visible and Infrared satellite imagery taken from radiometer instruments on SMS (ATS) and GOES satellites in geostationary orbit. These satellites produced...

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

Science.gov (United States)

Qian, Wei-Ping; Tan, Yue-Qiu; Chen, Ying; Peng, Ying; Li, Zhi; Lu, Guang-Xiu; Lin, Marie C.; Kung, Hsiang-Fu; He, Ming-Ling; Shing, Li-Ka

2005-01-01

AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS: Hepatitis B viral DNA was isolated from HBV carriers’ semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5 × 107 and 1.67 × 107 copies of HBV DNA per mL in two HBV infected patients’ sera, while 2.14 × 105 and 3.02 × 105 copies of HBV DNA per mL in the semen. CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. PMID:16149152

Theory of geostationary satellites

CERN Document Server

Zee, Chong-Hung

1989-01-01

Geostationary or equatorial synchronous satellites are a daily reminder of our space efforts during the past two decades. The nightly television satellite weather picture, the intercontinental telecommunications of television transmissions and telephone conversations, and the establishrnent of educational programs in remote regions on Earth are constant reminders of the presence of these satellites. As used here, the term 'geo­ stationary' must be taken loosely because, in the long run, the satellites will not remain 'stationary' with respect to an Earth-fixed reference frame. This results from the fact that these satellites, as is true for all satellites, are incessantly subject to perturbations other than the central-body attraction of the Earth. Among the more predominant pertur­ bations are: the ellipticity of the Earth's equator, the Sun and Moon, and solar radiation pressure. Higher harmonics of the Earth's potential and tidal effects also influence satellite motion, but they are of second­ order whe...

13 CFR 500.100 - Purpose and scope.

Science.gov (United States)

2010-01-01

... Board's authorities and organizational structure, the means and rules by which the Board takes actions... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Purpose and scope. 500.100 Section 500.100 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD EMERGENCY OIL AND...

13 CFR 500.213 - Termination of obligations.

Science.gov (United States)

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Termination of obligations. 500.213 Section 500.213 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD... the Application, the Guarantee or the Loan Documents; (5) A Lender fails to make a demand for payment...

49 CFR 179.500-10 - Protective housing.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Protective housing. 179.500-10 Section 179.500-10...-10 Protective housing. (a) Safety devices, and loading and unloading valves on tanks shall be protected from accidental damage by approved metal housing, arranged so it may be readily opened to permit...

Space Solar Power Satellite Systems, Modern Small Satellites, and Space Rectenna

Science.gov (United States)

Bergsrud, Corey Alexis Marvin

Space solar power satellite (SSPS) systems is the concept of placing large satellite into geostationary Earth orbit (GEO) to harvest and convert massive amounts of solar energy into microwave energy, and to transmit the microwaves to a rectifying antenna (rectenna) array on Earth. The rectenna array captures and converts the microwave power into usable power that is injected into the terrestrial electric grid for use. This work approached the microwave power beam as an additional source of power (with solar) for lower orbiting satellites. Assuming the concept of retrodirectivity, a GEO-SSPS antenna array system tracks and delivers microwave power to lower orbiting satellites. The lower orbiting satellites are equipped with a stacked photovoltaic (PV)/rectenna array hybrid power generation unit (HPGU) in order to harvest solar and/or microwave energy for on-board use during orbit. The area, and mass of the PV array part of the HPGU was reduced at about 32% beginning-of-life power in order to achieve the spacecraft power requirements. The HPGU proved to offer a mass decrease in the PGU, and an increase in mission life due to longer living component life of the rectenna array. Moreover, greater mission flexibility is achieved through a track and power delivery concept. To validate the potential advantages offered by a HPGU, a mission concept was presented that utilizes modern small satellites as technology demonstrators. During launch, a smaller power receiving "daughter" satellite sits inside a larger power transmitting "mother" satellite. Once separated from the launch vehicle the daughter satellite is ejected away from the mother satellite, and each satellite deploys its respective power transmitting or power receiving hardware's for experimentation. The concept of close proximity mission operations between the satellites is considered. To validate the technology of the space rectenna array part of the HPGU, six milestones were completed in the design. The first

TOP500 Supercomputers for November 2004

Energy Technology Data Exchange (ETDEWEB)

Strohmaier, Erich; Meuer, Hans W.; Dongarra, Jack; Simon, Horst D.

2004-11-08

24th Edition of TOP500 List of World's Fastest Supercomputers Released: DOE/IBM BlueGene/L and NASA/SGI's Columbia gain Top Positions MANNHEIM, Germany; KNOXVILLE, Tenn.; BERKELEY, Calif. In what has become a closely watched event in the world of high-performance computing, the 24th edition of the TOP500 list of the worlds fastest supercomputers was released today (November 8, 2004) at the SC2004 Conference in Pittsburgh, Pa.

TOP500 Supercomputers for June 2004

Energy Technology Data Exchange (ETDEWEB)

Strohmaier, Erich; Meuer, Hans W.; Dongarra, Jack; Simon, Horst D.

2004-06-23

23rd Edition of TOP500 List of World's Fastest Supercomputers Released: Japan's Earth Simulator Enters Third Year in Top Position MANNHEIM, Germany; KNOXVILLE, Tenn.;&BERKELEY, Calif. In what has become a closely watched event in the world of high-performance computing, the 23rd edition of the TOP500 list of the world's fastest supercomputers was released today (June 23, 2004) at the International Supercomputer Conference in Heidelberg, Germany.

TOP500 Supercomputers for June 2002

Energy Technology Data Exchange (ETDEWEB)

Strohmaier, Erich; Meuer, Hans W.; Dongarra, Jack; Simon, Horst D.

2002-06-20

19th Edition of TOP500 List of World's Fastest Supercomputers Released MANNHEIM, Germany; KNOXVILLE, Tenn.;&BERKELEY, Calif. In what has become a much-anticipated event in the world of high-performance computing, the 19th edition of the TOP500 list of the worlds fastest supercomputers was released today (June 20, 2002). The recently installed Earth Simulator supercomputer at the Earth Simulator Center in Yokohama, Japan, is as expected the clear new number 1. Its performance of 35.86 Tflop/s (trillions of calculations per second) running the Linpack benchmark is almost five times higher than the performance of the now No.2 IBM ASCI White system at Lawrence Livermore National Laboratory (7.2 Tflop/s). This powerful leap frogging to the top by a system so much faster than the previous top system is unparalleled in the history of the TOP500.

31 CFR 500.407 - Administration of blocked estates of decedents.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Administration of blocked estates of decedents. 500.407 Section 500.407 Money and Finance: Treasury Regulations Relating to Money and Finance... Interpretations § 500.407 Administration of blocked estates of decedents. Section 500.201 prohibits all...

SatelliteDL: a Toolkit for Analysis of Heterogeneous Satellite Datasets

Science.gov (United States)

Galloy, M. D.; Fillmore, D.

2014-12-01

SatelliteDL is an IDL toolkit for the analysis of satellite Earth observations from a diverse set of platforms and sensors. The core function of the toolkit is the spatial and temporal alignment of satellite swath and geostationary data. The design features an abstraction layer that allows for easy inclusion of new datasets in a modular way. Our overarching objective is to create utilities that automate the mundane aspects of satellite data analysis, are extensible and maintainable, and do not place limitations on the analysis itself. IDL has a powerful suite of statistical and visualization tools that can be used in conjunction with SatelliteDL. Toward this end we have constructed SatelliteDL to include (1) HTML and LaTeX API document generation,(2) a unit test framework,(3) automatic message and error logs,(4) HTML and LaTeX plot and table generation, and(5) several real world examples with bundled datasets available for download. For ease of use, datasets, variables and optional workflows may be specified in a flexible format configuration file. Configuration statements may specify, for example, a region and date range, and the creation of images, plots and statistical summary tables for a long list of variables. SatelliteDL enforces data provenance; all data should be traceable and reproducible. The output NetCDF file metadata holds a complete history of the original datasets and their transformations, and a method exists to reconstruct a configuration file from this information. Release 0.1.0 distributes with ingest methods for GOES, MODIS, VIIRS and CERES radiance data (L1) as well as select 2D atmosphere products (L2) such as aerosol and cloud (MODIS and VIIRS) and radiant flux (CERES). Future releases will provide ingest methods for ocean and land surface products, gridded and time averaged datasets (L3 Daily, Monthly and Yearly), and support for 3D products such as temperature and water vapor profiles. Emphasis will be on NPP Sensor, Environmental and

31 CFR 500.511 - Transactions by certain business enterprises.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Transactions by certain business enterprises. 500.511 Section 500.511 Money and Finance: Treasury Regulations Relating to Money and Finance... Licenses, Authorizations and Statements of Licensing Policy § 500.511 Transactions by certain business...

Interim Service ISDN Satellite (ISIS) network model for advanced satellite designs and experiments

Science.gov (United States)

Pepin, Gerard R.; Hager, E. Paul

1991-01-01

The Interim Service Integrated Services Digital Network (ISDN) Satellite (ISIS) Network Model for Advanced Satellite Designs and Experiments describes a model suitable for discrete event simulations. A top-down model design uses the Advanced Communications Technology Satellite (ACTS) as its basis. The ISDN modeling abstractions are added to permit the determination and performance for the NASA Satellite Communications Research (SCAR) Program.

Sea level trends in Southeast Asian seas

Science.gov (United States)

Strassburg, M. W.; Hamlington, B. D.; Leben, R. R.; Manurung, P.; Lumban Gaol, J.; Nababan, B.; Vignudelli, S.; Kim, K.-Y.

2015-05-01

Southeast Asian seas span the largest archipelago in the global ocean and provide a complex oceanic pathway connecting the Pacific and Indian oceans. The Southeast Asian sea regional sea level trends are some of the highest observed in the modern satellite altimeter record that now spans almost 2 decades. Initial comparisons of global sea level reconstructions find that 17-year sea level trends over the past 60 years exhibit good agreement with decadal variability associated with the Pacific Decadal Oscillation and related fluctuations of trade winds in the region. The Southeast Asian sea region exhibits sea level trends that vary dramatically over the studied time period. This historical variation suggests that the strong regional sea level trends observed during the modern satellite altimeter record will abate as trade winds fluctuate on decadal and longer timescales. Furthermore, after removing the contribution of the Pacific Decadal Oscillation (PDO) to sea level trends in the past 20 years, the rate of sea level rise is greatly reduced in the Southeast Asian sea region. As a result of the influence of the PDO, the Southeast Asian sea regional sea level trends during the 2010s and 2020s are likely to be less than the global mean sea level (GMSL) trend if the observed oscillations in wind forcing and sea level persist. Nevertheless, long-term sea level trends in the Southeast Asian seas will continue to be affected by GMSL rise occurring now and in the future.

Sea level trends in South East Asian Seas (SEAS)

Science.gov (United States)

Strassburg, M. W.; Hamlington, B. D.; Leben, R. R.; Manurung, P.; Lumban Gaol, J.; Nababan, B.; Vignudelli, S.; Kim, K.-Y.

2014-10-01

Southeast Asian Seas (SEAS) span the largest archipelago in the global ocean and provide a complex oceanic pathway connecting the Pacific and Indian Oceans. The SEAS regional sea level trends are some of the highest observed in the modern satellite altimeter record that now spans almost two decades. Initial comparisons of global sea level reconstructions find that 17 year sea level trends over the past 60 years exhibit good agreement in areas and at times of strong signal to noise associated decadal variability forced by low frequency variations in Pacific trade winds. The SEAS region exhibits sea level trends that vary dramatically over the studied time period. This historical variation suggests that the strong regional sea level trends observed during the modern satellite altimeter record will abate as trade winds fluctuate on decadal and longer time scales. Furthermore, after removing the contribution of the Pacific Decadal Oscillation (PDO) to sea level trends in the past twenty years, the rate of sea level rise is greatly reduced in the SEAS region. As a result of the influence of the PDO, the SEAS regional sea level trends during 2010s and 2020s are likely to be less than the global mean sea level (GMSL) trend if the observed oscillations in wind forcing and sea level persist. Nevertheless, long-term sea level trends in the SEAS will continue to be affected by GMSL rise occurring now and in the future.

49 CFR 179.500-6 - Heat treatment.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Heat treatment. 179.500-6 Section 179.500-6...-6 Heat treatment. (a) Each necked-down tank shall be uniformly heat treated. Heat treatment shall... treatment of alternate steels shall be approved. All scale shall be removed from outside of tank to an...

31 CFR 500.529 - Powers of attorney.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Powers of attorney. 500.529 Section 500.529 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) OFFICE OF... section does not authorize the creation of any power of attorney in favor of any person outside of the...

49 CFR 179.500-7 - Physical tests.

Science.gov (United States)

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false Physical tests. 179.500-7 Section 179.500-7...-7 Physical tests. (a) Physical tests shall be made on two test specimens 0.505 inch in diameter... tank. These test specimen ring sections or prolongations shall be heat treated, with the necked-down...

Career Development Programs in Fortune 500 Firms.

Science.gov (United States)

Keller, Jack; Piotrowski, Chris

Career development programs (CDPs) are a rather recent area of study in organizational and industrial psychology. The present study investigated the nature and evaluation of CDPs in Fortune 500 firms. Data were obtained by a mailed questionnaire completed by the firms' human resources directors. Of the 500 companies surveyed, only those 50 that…

Alkaline conditions stimulate the production of 1,3-propanediol in Lactobacillus panis PM1 through shifting metabolic pathways.

Science.gov (United States)

Grahame, Douglas A S; Kang, Tae Sun; Khan, Nurul H; Tanaka, Takuji

2013-07-01

A novel Lactobacillus panis PM1 isolate was found to be capable of converting glycerol to 1,3-propanediol (1,3-PDO), an increasingly valuable commodity chemical. In this study the effects of various process parameters, including glucose and glycerol concentrations, inoculum size, temperature, aeration, pH, and carbon source were examined to determine the optimal conditions for the production of 1,3-PDO using a culture method simulating late log to early stationary phases. Inoculum size did not influence the production of 1,3-PDO, and temperature variance showed similar 1,3-PDO production between 25 and 37 °C under the examined conditions. Glycerol concentration and pH played a primary role in the final concentration of 1,3-PDO. The highest production occurred at 150-250 mM glycerol when 50 mM glucose was available. Alkaline initial conditions (pH 9-10) stimulated the production of 1,3-PDO which concurrently occurred with increased acetic acid production. Under these conditions, 213.6 mM of 1,3-PDO were produced from 300 mM glycerol (conversion efficiency was 71 %). These observations indicated that the production of 1,3-PDO was associated with the shift of the metabolic end-product ethanol to acetic acid, and that this shift resulted in an excess concentration of NADH available for the processing of glycerol to 1,3-PDO.

Evaluation of a Structured Predeparture Orientation at the David Geffen School of Medicine's Global Health Education Programs.

Science.gov (United States)

Herbst de Cortina, Sasha; Arora, Gitanjli; Wells, Traci; Hoffman, Risa M

2016-03-01

Given the lack of a standardized approach to medical student global health predeparture preparation, we evaluated an in-person, interactive predeparture orientation (PDO) at the University of California Los Angeles (UCLA) to understand program strengths, weaknesses, and areas for improvement. We administered anonymous surveys to assess the structure and content of the PDO and also surveyed a subset of students after travel on the utility of the PDO. We used Fisher's exact test to evaluate the association between prior global health experience and satisfaction with the PDO. One hundred and five students attended the PDO between 2010 and 2014 and completed the survey. One hundred and four students (99.0%) reported learning new information. Major strengths included faculty mentorship (N = 38, 19.7%), opportunities to interact with the UCLA global health community (N = 34, 17.6%), and sharing global health experiences (N = 32, 16.6%). Of students surveyed after their elective, 94.4% (N = 51) agreed or strongly agreed that the PDO provided effective preparation. Students with prior global health experience found the PDO to be as useful as students without experience (92.7% versus 94.4%, P = 1.0). On the basis of these findings, we believe that a well-composed PDO is beneficial for students participating in global health experiences and recommend further comparative studies of PDO content and delivery. © The American Society of Tropical Medicine and Hygiene.

TOP500 Supercomputers for June 2005

Energy Technology Data Exchange (ETDEWEB)

Strohmaier, Erich; Meuer, Hans W.; Dongarra, Jack; Simon, Horst D.

2005-06-22

25th Edition of TOP500 List of World's Fastest Supercomputers Released: DOE/L LNL BlueGene/L and IBM gain Top Positions MANNHEIM, Germany; KNOXVILLE, Tenn.; BERKELEY, Calif. In what has become a closely watched event in the world of high-performance computing, the 25th edition of the TOP500 list of the world's fastest supercomputers was released today (June 22, 2005) at the 20th International Supercomputing Conference (ISC2005) in Heidelberg Germany.

Ionizing radiation risks to Satellite Power Systems (SPS) workers in space

Energy Technology Data Exchange (ETDEWEB)

1980-12-01

A reference Satellite Power System (SPS) has been designed by NASA and its contractors for the purposes of evaluating the concept and carrying out assessments of the various consequences of development, including those on the health of the space workers. The Department of Energy has responsibility for directing various assessments. Present planning calls for the SPS workers to move from Earth to a low earth orbit (LEO) at an altitude of 500 kilometers; to travel by a transfer ellipse (TE) trajectory to a geosynchronous orbit (GEO) at an altitude of 36,000 kilometers; and to remain in GEO orbit for about 90 percent of the total time aloft. The radiation risks to the health of workers who will construct and maintain solar power satellites in the space environment are studied. The charge to the committee was: (a) to evaluate the radiation environment estimated for the Reference System which could represent a hazard; (b) to assess the possible somatic and genetic radiation hazards; and (c) to estimate the risks to the health of SPS workers due to space radiation exposure, and to make recommendations based on these conclusions. Details are presented. (WHK)

29 CFR 500.81 - Payment of wages when due.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Payment of wages when due. 500.81 Section 500.81 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND SEASONAL AGRICULTURAL WORKER PROTECTION Worker Protections Wages and Payroll Standards § 500.81 Payment of...

Template DNA-strand co-segregation and asymmetric cell division in skeletal muscle stem cells.

Science.gov (United States)

Shinin, Vasily; Gayraud-Morel, Barbara; Tajbakhsh, Shahragim

2009-01-01

Stem cells are present in all tissues and organs, and are crucial for normal regulated growth. How the pool size of stem cells and their progeny is regulated to establish the tissue prenatally, then maintain it throughout life, is a key question in biology and medicine. The ability to precisely locate stem and progenitors requires defining lineage progression from stem to differentiated cells, assessing the mode of cell expansion and self-renewal and identifying markers to assess the different cell states within the lineage. We have shown that during lineage progression from a quiescent adult muscle satellite cell to a differentiated myofibre, both symmetric and asymmetric divisions take place. Furthermore, we provide evidence that a sub-population of label retaining satellite cells co-segregate template DNA strands to one daughter cell. These findings provide a means of identifying presumed stem and progenitor cells within the lineage. In addition, asymmetric segregation of template DNA and the cytoplasmic protein Numb provides a landmark to define cell behaviour as self-renewal and differentiation decisions are being executed.

Satellite Communications

CERN Document Server

Pelton, Joseph N

2012-01-01

The field of satellite communications represents the world's largest space industry. Those who are interested in space need to understand the fundamentals of satellite communications, its technology, operation, business, economic, and regulatory aspects. This book explains all this along with key insights into the field's future growth trends and current strategic challenges. Fundamentals of Satellite Communications is a concise book that gives all of the key facts and figures as well as a strategic view of where this dynamic industry is going. Author Joseph N. Pelton, PhD, former Dean of the International Space University and former Director of Strategic Policy at Intelstat, presents a r

microRNA Biomarker Discovery and High-Throughput DNA Sequencing Are Possible Using Long-term Archived Serum Samples.

Science.gov (United States)

Rounge, Trine B; Lauritzen, Marianne; Langseth, Hilde; Enerly, Espen; Lyle, Robert; Gislefoss, Randi E

2015-09-01

The impacts of long-term storage and varying preanalytical factors on the quality and quantity of DNA and miRNA from archived serum have not been fully assessed. Preanalytical and analytical variations and degradation may introduce bias in representation of DNA and miRNA and may result in loss or corruption of quantitative data. We have evaluated DNA and miRNA quantity, quality, and variability in samples stored up to 40 years using one of the oldest prospective serum collections in the world, the Janus Serumbank, a biorepository dedicated to cancer research. miRNAs are present and stable in archived serum samples frozen at -25°C for at least 40 years. Long-time storage did not reduce miRNA yields; however, varying preanalytical conditions had a significant effect and should be taken into consideration during project design. Of note, 500 μL serum yielded sufficient miRNA for qPCR and small RNA sequencing and on average 650 unique miRNAs were detected in samples from presumably healthy donors. Of note, 500 μL serum yielded sufficient DNA for whole-genome sequencing and subsequent SNP calling, giving a uniform representation of the genomes. DNA and miRNA are stable during long-term storage, making large prospectively collected serum repositories an invaluable source for miRNA and DNA biomarker discovery. Large-scale biomarker studies with long follow-up time are possible utilizing biorepositories with archived serum and state-of-the-art technology. ©2015 American Association for Cancer Research.

The American Satellite Company (ASC) satellite deployed from payload bay

Science.gov (United States)

1985-01-01

The American Satellite Company (ASC) communications satellite is deployed from the payload bay of the Shuttle Discovery. A portion of the cloudy surface of the earth can be seen to the left of the frame.

42 CFR 456.500 - Purpose.

Science.gov (United States)

2010-10-01

... ASSISTANCE PROGRAMS UTILIZATION CONTROL Utilization Review Plans: FFP, Waivers, and Variances for Hospitals and Mental Hospitals § 456.500 Purpose. For hospitals and mental hospitals, this subpart— (a...

A combined method for DNA analysis and radiocarbon dating from a single sample.

Science.gov (United States)

Korlević, Petra; Talamo, Sahra; Meyer, Matthias

2018-03-07

Current protocols for ancient DNA and radiocarbon analysis of ancient bones and teeth call for multiple destructive samplings of a given specimen, thereby increasing the extent of undesirable damage to precious archaeological material. Here we present a method that makes it possible to obtain both ancient DNA sequences and radiocarbon dates from the same sample material. This is achieved by releasing DNA from the bone matrix through incubation with either EDTA or phosphate buffer prior to complete demineralization and collagen extraction utilizing the acid-base-acid-gelatinization and ultrafiltration procedure established in most radiocarbon dating laboratories. Using a set of 12 bones of different ages and preservation conditions we demonstrate that on average 89% of the DNA can be released from sample powder with minimal, or 38% without any, detectable collagen loss. We also detect no skews in radiocarbon dates compared to untreated samples. Given the different material demands for radiocarbon dating (500 mg of bone/dentine) and DNA analysis (10-100 mg), combined DNA and collagen extraction not only streamlines the sampling process but also drastically increases the amount of DNA that can be recovered from limited sample material.

Trends in mobile satellite communication

Science.gov (United States)

Johannsen, Klaus G.; Bowles, Mike W.; Milliken, Samuel; Cherrette, Alan R.; Busche, Gregory C.

1993-01-01

Ever since the U.S. Federal Communication Commission opened the discussion on spectrum usage for personal handheld communication, the community of satellite manufacturers has been searching for an economically viable and technically feasible satellite mobile communication system. Hughes Aircraft Company and others have joined in providing proposals for such systems, ranging from low to medium to geosynchronous orbits. These proposals make it clear that the trend in mobile satellite communication is toward more sophisticated satellites with a large number of spot beams and onboard processing, providing worldwide interconnectivity. Recent Hughes studies indicate that from a cost standpoint the geosynchronous satellite (GEOS) is most economical, followed by the medium earth orbit satellite (MEOS) and then by the low earth orbit satellite (LEOS). From a system performance standpoint, this evaluation may be in reverse order, depending on how the public will react to speech delay and collision. This paper discusses the trends and various mobile satellite constellations in satellite communication under investigation. It considers the effect of orbital altitude and modulation/multiple access on the link and spacecraft design.

JPSS Preparations at the Satellite Proving Ground for Marine, Precipitation, and Satellite Analysis

Science.gov (United States)

Folmer, Michael J.; Berndt, E.; Clark, J.; Orrison, A.; Kibler, J.; Sienkiewicz, J.; Nelson, J.; Goldberg, M.; Sjoberg, W.

2016-01-01

The ocean prediction center at the national hurricane center's tropical analysis and forecast Branch, the Weather Prediction center and the Satellite analysis branch of NESDIS make up the Satellite Proving Ground for Marine, Precipitation and Satellite Analysis. These centers had early exposure to JPSS products using the S-NPP Satellite that was launched in 2011. Forecasters continue to evaluate new products in anticipation for the launch of JPSS-1 sometime in 2017.

25 CFR 11.500 - Law applicable to civil actions.

Science.gov (United States)

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Law applicable to civil actions. 11.500 Section 11.500 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAW AND ORDER COURTS OF INDIAN OFFENSES AND LAW AND ORDER CODE Civil Actions § 11.500 Law applicable to civil actions. (a) In all civil cases, the...

The satellite situation center

International Nuclear Information System (INIS)

Teague, M.J.; Sawyer, D.M.; Vette, J.I.

1982-01-01

Considerations related to the early planning for the International Magnetospheric Study (IMS) took into account the desirability of an establishment of specific entities for generating and disseminating coordination information for both retrospective and predictive periods. The organizations established include the IMS/Satellite Situation Center (IMS/SSC) operated by NASA. The activities of the SSC are related to the preparation of reports on predicted and actually achieved satellite positions, the response to inquiries, the compilation of information on satellite experiments, and the issue of periodic status summaries. Attention is given to high-altitude satellite services, other correlative satellite services, non-IMS activities of the SSC, a summary of the SSC request activity, and post-IMS and future activities

Plan of Time Management of Satellite Positioning System using Quasi-zenith Satellite

Science.gov (United States)

Takahashi, Yasuhiro; Fujieda, Miho; Amagai, Jun; Yokota, Shoichiro; Kimura, Kazuhiro; Ito, Hiroyuki; Hama, Shin'ichi; Morikawa, Takao; Kawano, Isao; Kogure, Satoshi

The Quasi-Zenith satellites System (QZSS) is developed as an integrated satellite service system of communication, broadcasting and positioning for mobile users in specified regions of Japan from high elevation angle. Purposes of the satellite positioning system using Quasi-Zenith satellite (QZS) are to complement and augment the GPS. The national institutes concerned have been developing the positioning system using QZS since 2003 and will carry out experiments and researches in three years after the launch. In this system, National Institute of Information and Communications Technology (NICT) is mainly in charge of timing system for the satellite positioning system using QZS, such as onboard hydrogen maser atomic clock and precise time management system of the QZSS. We started to develop the engineering model of the time management system for the QZSS. The time management system for the QZSS will be used to compare time differences between QZS and earth station as well as to compare between three onboard atomic clocks. This paper introduces time management of satellite positioning system using the QZSS.

W-enriched satellite sequence in the Indian meal moth, Plodia interpunctella (Lepidoptera, Pyralidae)

Czech Academy of Sciences Publication Activity Database

Dalíková, Martina; Zrzavá, Magda; Kubíčková, S.; Marec, František

2017-01-01

Roč. 25, 3-4 (2017), s. 241-252 ISSN 0967-3849 R&D Projects: GA ČR(CZ) GA14-22765S; GA ČR(CZ) GA17-13713S Grant - others:GA ČR(CZ) GA17-17211S Institutional support: RVO:60077344 Keywords : satellite DNA * sex chromosomes * transcription Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Genetics and heredity (medical genetics to be 3) Impact factor: 2.385, year: 2016 https://link.springer.com/article/10.1007%2Fs10577-017-9558-8

New visible and selective DNA staining method in gels with tetrazolium salts.

Science.gov (United States)

Paredes, Aaron J; Naranjo-Palma, Tatiana; Alfaro-Valdés, Hilda M; Barriga, Andrés; Babul, Jorge; Wilson, Christian A M

2017-01-15

DNA staining in gels has historically been carried out using silver staining and fluorescent dyes like ethidium bromide and SYBR Green I (SGI). Using fluorescent dyes allows recovery of the analyte, but requires instruments such as a transilluminator or fluorimeter to visualize the DNA. Here we described a new and simple method that allows DNA visualization to the naked eye by generating a colored precipitate. It works by soaking the acrylamide or agarose DNA gel in SGI and nitro blue tetrazolium (NBT) solution that, when exposed to sunlight, produces a purple insoluble formazan precipitate that remains in the gel after exposure to light. A calibration curve made with a DNA standard established a detection limit of approximately 180 pg/band at 500 bp. Selectivity of this assay was determined using different biomolecules, demonstrating a high selectivity for DNA. Integrity and functionality of the DNA recovered from gels was determined by enzymatic cutting with a restriction enzyme and by transforming competent cells after the different staining methods, respectively. Our method showed the best performance among the dyes employed. Based on its specificity, low cost and its adequacy for field work, this new methodology has enormous potential benefits to research and industry. Copyright © 2016 Elsevier Inc. All rights reserved.

Demonstration of the 500 kW stoker burning system; 500 kW stokeripolttojaerjestelmaen demonstrointi

Energy Technology Data Exchange (ETDEWEB)

Oravainen, H. [VTT Energy, Jyvaeskylae (Finland); Kantalainen, K. [Hoegfors Laempoe Oy, Saarijaervi (Finland)

1995-12-31

The objective of the project is to demonstrate the operation of a 500 kW stoker-burning system in practice. The project is continuation of the previous projects of the Bioenergy research programme, 303 - Development of automatic heating system for wood chips and sod peat, carried out by VTT Energy, and Y301 - Development of heating boiler for wood chips and sod peat, carried out by Hoegfors Laempoe Oy. A 500 kW (nominal power) stoker-burner was constructed on the basis of the results of these projects. The burner was mounted on ETNA 500 bioenergy boiler. Screw-feeders, constructed by Maamiehen Saehkoe Oy, were used as fuel feeding system. Maamiehen Saehkoe Oy delivered also the automation system for the combustion equipment. Combustion air pre-heater was mounted on the boiler for promotion of the combustion of moist fuel. Testing of the equipment was carried out at the laboratory of VTT Energy in Jyvaeskylae in October-November 1994. In December 1994 the equipment was transported to Jalasjaervi, to heating station of the farmer Juha Jyrae. The actual heat generation started in the beginning of February 1995, when the greenhouses started to require heating. Sod peat has been used as the fuel. VTT Energy has carried out the efficiency and emission measurements in the heating station with sod peat in March 1995, and with reed canary grass in autumn 1995. The energy generation and fuel consumption have been followed all the time

Demonstration of the 500 kW stoker burning system; 500 kW stokeripolttojaerjestelmaen demonstrointi

Energy Technology Data Exchange (ETDEWEB)

Oravainen, H [VTT Energy, Jyvaeskylae (Finland); Kantalainen, K [Hoegfors Laempoe Oy, Saarijaervi (Finland)

1996-12-31

The objective of the project is to demonstrate the operation of a 500 kW stoker-burning system in practice. The project is continuation of the previous projects of the Bioenergy research programme, 303 - Development of automatic heating system for wood chips and sod peat, carried out by VTT Energy, and Y301 - Development of heating boiler for wood chips and sod peat, carried out by Hoegfors Laempoe Oy. A 500 kW (nominal power) stoker-burner was constructed on the basis of the results of these projects. The burner was mounted on ETNA 500 bioenergy boiler. Screw-feeders, constructed by Maamiehen Saehkoe Oy, were used as fuel feeding system. Maamiehen Saehkoe Oy delivered also the automation system for the combustion equipment. Combustion air pre-heater was mounted on the boiler for promotion of the combustion of moist fuel. Testing of the equipment was carried out at the laboratory of VTT Energy in Jyvaeskylae in October-November 1994. In December 1994 the equipment was transported to Jalasjaervi, to heating station of the farmer Juha Jyrae. The actual heat generation started in the beginning of February 1995, when the greenhouses started to require heating. Sod peat has been used as the fuel. VTT Energy has carried out the efficiency and emission measurements in the heating station with sod peat in March 1995, and with reed canary grass in autumn 1995. The energy generation and fuel consumption have been followed all the time

Noteworthy: Fortune 500: Texas ties California for national lead

OpenAIRE

Michael Nicholson

2010-01-01

According to the 2010 Fortune 500, released in April, Texas hosts the headquarters of 57 of the nation's 500 largest companies, ranked by gross revenues. Texas secured its place as a Fortune 500 leader through its position as focal point of the domestic energy industry, its relatively strong economic growth over the past decade, and its relatively low tax rates and living costs.

Construction and characterization of cDNA library for IRM-2 mice

International Nuclear Information System (INIS)

Wang Qin; Li Jin; Song Li; Liu Qiang; Yue Jingyin; Mu Chuanjie; Tang Weisheng; Fan Feiyue

2010-01-01

Objective: To screen and isolate the radioresistance related genes of IRM-2 mice. Methods: cDNA library of IRM-2 mice was constructed by SMART technique. Total RNA was isolated from spleens of IRM-2 male mice. The first-strand cDNA was synthesized by using PowerScript reverse transcriptase, and double-strand cDNA was synthesized and amplified by long PCR. The PCR products were purified, digested with restriction enzyme Sfi I. The ds-cDNA fragment less than 500 bp was fractionated and ligated to the Sfi I-digested pDNR-LIB vector. The ligation mixture was transformed into E. coil DH5 α by electroporation transformation to generate the unamplified cDNA library. The quality of cDNA library was identified by PCR technique. 130 clones from cDNA library were sequenced and compared with GenBank database. Results: The cDNA library contained 2.25 x 10 6 independent clones with an average insert size of 1.2 kb. The ratio of recombination and full-length was 95% and 55%, respectively. 21 pieces of EST sequences from cDNA library were not the same as the known mice genes and registered into GenBank EST database, with registered number DW474856-DW474876. Conclusions: cDNA library of IRM-2 mice has been constructed successfully. 21 pieces of EST implies that radioresistance correlative genes may be in IRM-2 mice, which will lay a foundation for isolating and identifying radioresistance related genes in further study. (authors)

Isolation and partial characterization of a new strain of Klebsiella pneumoniae capable of high 1,3 propanediol production from glycerol

Directory of Open Access Journals (Sweden)

B. Sen

2015-04-01

Full Text Available Glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 PDO. Here we report a newly isolated bacterial strain BA11 from soil, capable of fermenting glycerol to 1,3 PDO, and has been identified to be a strain of Klebsiella pneumoniae. Strain BA11 was fast growing showing peak 1,3 PDO production in 6 h of cultivation with productivity of 1.2 g/L-h without the addition of Vitamin B12. Based on the optimum glycerol utilization (75% and 1,3 PDO production (8.3 g/L and yield (0.56 mol/mol glycerol utilized, the most appropriate glycerol concentration for cultivation was 20 g/L. The strain BA11 could tolerate the pH range of 6 to 8.5 as no inhibitory effects were seen on growth as well as 1,3 PDO production. Strain BA11 was most active and could produce high 1,3 PDO in the incubation temperature range of 25 to 40 oC. The production of 1,3 PDO was maximum (9.3 g/L under aerobic condition with 95.8% glycerol utilization. Addition of glucose to the glycerol fermentation led to increased cell mass but no improvement in the 1,3 PDO production.

Clustered DNA damage induced by proton and heavy ion irradiation

International Nuclear Information System (INIS)

Davidkova, M.; Pachnerova Brabcova, K; Stepan, V.; Vysin, L.; Sihver, L.; Incerti, S.

2014-01-01

Ionizing radiation induces in DNA strand breaks, damaged bases and modified sugars, which accumulate with increasing density of ionizations in charged particle tracks. Compared to isolated DNA damage sites, the biological toxicity of damage clusters can be for living cells more severe. We investigated the clustered DNA damage induced by protons (30 MeV) and high LET radiation (C 290 MeV/u and Fe 500 MeV/u) in pBR322 plasmid DNA. To distinguish between direct and indirect pathways of radiation damage, the plasmid was irradiated in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The goal of the contribution is the analysis of determined types of DNA damage in dependence on radiation quality and related contribution of direct and indirect radiation effects. The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers. (authors)

Metagenomic and satellite analyses of red snow in the Russian Arctic

Directory of Open Access Journals (Sweden)

Nao Hisakawa

2015-12-01

Full Text Available Cryophilic algae thrive in liquid water within snow and ice in alpine and polar regions worldwide. Blooms of these algae lower albedo (reflection of sunlight, thereby altering melting patterns (Kohshima, Seko & Yoshimura, 1993; Lutz et al., 2014; Thomas & Duval, 1995. Here metagenomic DNA analysis and satellite imaging were used to investigate red snow in Franz Josef Land in the Russian Arctic. Franz Josef Land red snow metagenomes confirmed that the communities are composed of the autotroph Chlamydomonas nivalis that is supporting a complex viral and heterotrophic bacterial community. Comparisons with white snow communities from other sites suggest that white snow and ice are initially colonized by fungal-dominated communities and then succeeded by the more complex C. nivalis-heterotroph red snow. Satellite image analysis showed that red snow covers up to 80% of the surface of snow and ice fields in Franz Josef Land and globally. Together these results show that C. nivalis supports a local food web that is on the rise as temperatures warm, with potential widespread impacts on alpine and polar environments worldwide.

17 CFR 201.500 - Expedited consideration of proceedings.

Science.gov (United States)

2010-04-01

... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Expedited consideration of proceedings. 201.500 Section 201.500 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION... Expedited consideration of proceedings. Consistent with the Commission's or the hearing officer's other...

The Future of Satellite Communications Technology.

Science.gov (United States)

Nowland, Wayne

1985-01-01

Discusses technical advances in satellite technology since the 1960s, and the International Telecommunications Satellite Organization's role in these developments; describes how AUSSAT, Australia's domestic satellite system, exemplifies the latest developments in satellite technology; and reviews satellite system features, possible future…

12 CFR 500.10 - The OTS or The Office.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS General Organization § 500.10 The OTS or The Office. The Office of Thrift Supervision (referred to as “OTS” or “Office”) is an office of the...

[Satellite RNA (RNA3) of tomato black ring virus is found with one of the 2 major RNAs (RNA2) in a new capsid nucleoprotein].

Science.gov (United States)

Doz, B; Dunez, J; Bove, J M

1977-12-19

Tomato Black Ring Virus (TBRV) like other NEPOviruses posseses two nucleoproteins M and B and two major RNAs, RNA1 and RNA2 respectively distributed in B and M. A new nucleoprotein has just been discovered and comprises one molecule of RNA2 associated with one molecule of RNA3. RNA3 is a small RNA of molecular weight 500,000 d considered to be a satellite RNA. Its level appears to depend on the infection stage, local or systemic. RNA3 is able to modify the relative proportions of nucleoproteins M and B and their respective RNAs. The satellite RNA, might be part of the genome and represent a monocistronic mRNA for protein capsid synthesis. However it seems perhaps more tempting to correlate TBRV-RNA3 with satellite RNA5 of certain strains of Cucumber mosaic virus.

Cooperative and cognitive satellite systems

CERN Document Server

Chatzinotas, Symeon; De Gaudenzi, Riccardo

2015-01-01

Cooperative and Cognitive Satellite Systems provides a solid overview of the current research in the field of cooperative and cognitive satellite systems, helping users understand how to incorporate state-of-the-art communication techniques in innovative satellite network architectures to enable the next generation of satellite systems. The book is edited and written by top researchers and practitioners in the field, providing a comprehensive explanation of current research that allows users to discover future technologies and their applications, integrate satellite and terrestrial systems

Harman inhibits the removal of pyrimidine dimers from the DNA of human cells

International Nuclear Information System (INIS)

Castellani, A.; Setlow, R.B.

1981-01-01

Normal human fibroblasts were UV-irradiated and incubated for 6 hr with harman. The losses of sites, in the extracted DNA, sensitive to a UV specific endonuclease were determined as precision measures of the excision of UV-induced pyrimidine dimers. Harman inhibited excision, rising from approx. 30% inhibition at 200 μM to 75% inhibition at 500 μM

Protective effects of lemongrass (Cymbopogon citratus STAPF) essential oil on DNA damage and carcinogenesis in female Balb/C mice.

Science.gov (United States)

Bidinotto, Lucas T; Costa, Celso A R A; Salvadori, Daisy M F; Costa, Mirtes; Rodrigues, Maria A M; Barbisan, Luís F

2011-08-01

This study investigated the protective effect of oral treatment with lemongrass (Cymbopogon citratus STAPF) essential oil (LGEO) on leukocyte DNA damage induced by N-methyl-N-nitrosurea (MNU). Also, the anticarcinogenic activity of LGEO was investigated in a multi-organ carcinogenesis bioassay induced by 7,12-dimethylbenz(a)antracene, 1,2-dimethylhydrazine and N-butyl-N-(4-hydroxibuthyl)nitrosamine in Balb/C female Balb/c mice (DDB-initiated mice). In the short-term study, the animals were allocated into three groups: vehicle group (negative control), MNU group (positive control) and LGEO 500 mg kg⁻¹ (five times per week for 5 weeks) plus MNU group (test group). Blood samples were collected to analyze leukocyte DNA damage by comet assay 4 h after each MNU application at the end of weeks 3 and 5. The LGEO 500 mg kg⁻¹ treated group showed significantly lower (P lemongrass essential oil provided protective action against MNU-induced DNA damage and a potential anticarcinogenic activity against mammary carcinogenesis in DDB-initiated female Balb/C mice. Copyright © 2010 John Wiley & Sons, Ltd.

Satellite Communications for ATM

Science.gov (United States)

Shamma, Mohammed A.

2003-01-01

This presentation is an overview on Satellite Communication for the Aeronautical Telecommunication Management (ATM) research. Satellite Communications are being considered by the FAA and NASA as a possible alternative to the present and future ground systems supporting Air Traffic Communications. The international Civil Aviation Organization (ICAO) have in place Standards and Recommended Practices (SARPS) for the Aeronautical Mobile Satellite Services (AMSS) which is mainly derived from the pre-existing Inmarsat service that has been in service since the 1980s. The Working Group A of the Aeronautical Mobile Communication Panel of ICAO has also been investigating SARPS for what is called the Next Generation Satellite Service (NGSS) which conforms less to the Inmarsat based architecture and explores wider options in terms of satellite architectures. Several designs are being proposed by Firms such as Boeing, ESA, NASA that are geared toward full or secondary usage of satellite communications for ATM. Satellite communications for ATM can serve several purposes ranging from primary usage where ground services would play a minimal backup role, to an integrated solution where it will be used to cover services, or areas that are less likely to be supported by the proposed and existing ground infrastructure. Such Integrated roles can include usage of satellite communications for oceanic and remote land areas for example. It also can include relieving the capacity of the ground network by providing broadcast based services of Traffic Information Services messages (TIS-B), or Flight Information Services (FIS-B) which can take a significant portion of the ground system capacity. Additionally, satellite communication can play a backup role to support any needs for ground replacement, or additional needed capacity even after the new digital systems are in place. The additional bandwidth that can be provided via satellite communications can also open the door for many new

The histone H3K9 methylation and RNAi pathways regulate normalnucleolar and repeated DNA organization by inhibiting formation ofextrachromosomal DNAs

Energy Technology Data Exchange (ETDEWEB)

Peng, Jamy C.; Karpen, Gary H.

2006-06-15

In order to identify regulators of nuclear organization, Drosophila mutants in the Su(var)3-9 histone H3K9 methyltransferase, RNAi pathway components, and other regulators of heterochromatin-mediated gene silencing were examined for altered nucleoli and positioning of repeated DNAs. Animals lacking components of the H3K9 methylation and RNAi pathways contained disorganized nucleoli, ribosomal DNA (rDNA) and satellite DNAs. The levels of H3K9 dimethylation (H3K9me2) in chromatin associated with repeated DNAs decreased dramatically in Su(var)3-9 and dcr-2 (dicer-2) mutant tissues compared to wild type. We also observed a substantial increase in extrachromosomal repeated DNAs in mutant tissues. The disorganized nucleolus phenotype depends on the presence of Ligase 4 (Lig4), and ecc DNA formation is not induced by removal of cohesin. We conclude that H3K9 methylation of rDNA and satellites, maintained by Su(var)3-9, HP1, and the RNAi pathway, is necessary for the structural stability of repeated DNAs, which is mediated through suppression of non-homologous end joining (NHEJ). These results suggest a mechanism for how local chromatin structure can regulate genome stability, and the organization of chromosomal elements and nuclear organelles.

Distribution characteristics of energetic electron according to data of Interkosmos-17 satellite

International Nuclear Information System (INIS)

Martynov, A.I.; Makhmutov, V.S.; Petrov, V.M.; Shurshakov, V.A.

1990-01-01

Distributions of quasi-captured and precipitating electrons with E=10-70 keV within L=3-15 by local time and local pitch-angle for different conditions of magnetic perturbation are studied using measurement data from Interkosmos-17 satellite (polar circular orbit, h∼500 km). Dependence of electron flows on local time obtained for K p >1 is close to corresponding dependence from AE-4 model, and level of magnetosphere perturbation affects character of this dependence. Electron distributions by invariant latitude in the Earth polar cap are calculated at different levels of geomagnetic perturbation; electron flows during strong magnetic perturbations (K p >4) increase by ∼2 times, in this case, space heterogeneity of their distribution is intensified

Thematic mapping from satellite imagery

CERN Document Server

Denègre, J

2013-01-01

Thematic Mapping from Satellite Imagery: A Guidebook discusses methods in producing maps using satellite images. The book is comprised of five chapters; each chapter covers one stage of the process. Chapter 1 tackles the satellite remote sensing imaging and its cartographic significance. Chapter 2 discusses the production processes for extracting information from satellite data. The next chapter covers the methods for combining satellite-derived information with that obtained from conventional sources. Chapter 4 deals with design and semiology for cartographic representation, and Chapter 5 pre

Full Service ISDN Satellite (FSIS) network model for advanced ISDN satellite design and experiments

Science.gov (United States)

Pepin, Gerard R.

1992-01-01

The Full Service Integrated Services Digital Network (FSIS) network model for advanced satellite designs describes a model suitable for discrete event simulations. A top down model design uses the Advanced Communications Technology Satellite (ACTS) as its basis. The ACTS and the Interim Service ISDN Satellite (ISIS) perform ISDN protocol analyses and switching decisions in the terrestrial domain, whereas FSIS makes all its analyses and decisions on-board the ISDN satellite.

Genome organization and DNA methylation patterns of B chromosomes in the red fox and Chinese raccoon dogs.

Science.gov (United States)

Bugno-Poniewierska, Monika; Solek, Przemysław; Wronski, Mariusz; Potocki, Leszek; Jezewska-Witkowska, Grażyna; Wnuk, Maciej

2014-12-01

The molecular structure of B chromosomes (Bs) is relatively well studied. Previous research demonstrates that Bs of various species usually contain two types of repetitive DNA sequences, satellite DNA and ribosomal DNA, but Bs also contain genes encoding histone proteins and many others. However, many questions remain regarding the origin and function of these chromosomes. Here, we focused on the comparative cytogenetic characteristics of the red fox and Chinese raccoon dog B chromosomes with particular attention to the distribution of repetitive DNA sequences and their methylation status. We confirmed that the small Bs of the red fox show a typical fluorescent telomeric distal signal, whereas medium-sized Bs of the Chinese raccoon dog were characterized by clusters of telomeric sequences along their length. We also found different DNA methylation patterns for the B chromosomes of both species. Therefore, we concluded that DNA methylation may maintain the transcriptional inactivation of DNA sequences localized to B chromosomes and may prevent genetic unbalancing and several negative phenotypic effects. © 2014 The Authors.

TOP500 Supercomputers for November 2003

Energy Technology Data Exchange (ETDEWEB)

Strohmaier, Erich; Meuer, Hans W.; Dongarra, Jack; Simon, Horst D.

2003-11-16

22nd Edition of TOP500 List of World s Fastest Supercomputers Released MANNHEIM, Germany; KNOXVILLE, Tenn.; BERKELEY, Calif. In what has become a much-anticipated event in the world of high-performance computing, the 22nd edition of the TOP500 list of the worlds fastest supercomputers was released today (November 16, 2003). The Earth Simulator supercomputer retains the number one position with its Linpack benchmark performance of 35.86 Tflop/s (''teraflops'' or trillions of calculations per second). It was built by NEC and installed last year at the Earth Simulator Center in Yokohama, Japan.

500 Cities: Census Tract Boundaries

Data.gov (United States)

U.S. Department of Health & Human Services — This census tract shapefile for the 500 Cities project was extracted from the Census 2010 Tiger/Line database and modified to remove portions of census tracts that...

Unique structural properties of DNA interstrand cross-links formed by a new antitumor dinuclear Pt(II) complex

Czech Academy of Sciences Publication Activity Database

Hrabina, O.; Kašpárková, J.; Suchánková, Tereza; Novohradský, Vojtěch; Guo, Z.; Brabec, Viktor

2017-01-01

Roč. 9, č. 5 (2017), s. 494-500 ISSN 1756-5901 Institutional support: RVO:68081707 Keywords : cisplatin-modified dna * nucleotide excision-repair * hmg domain proteins Subject RIV: CE - Biochemistry OBOR OECD: Biochemistry and molecular biology Impact factor: 3.975, year: 2016

A modular metabolic engineering approach for the production of 1,2-propanediol from glycerol by Saccharomyces cerevisiae.

Science.gov (United States)

Islam, Zia-Ul; Klein, Mathias; Aßkamp, Maximilian R; Ødum, Anders S R; Nevoigt, Elke

2017-11-01

Compared to sugars, a major advantage of using glycerol as a feedstock for industrial bioprocesses is the fact that this molecule is more reduced than sugars. A compound whose biotechnological production might greatly profit from the substrate's higher reducing power is 1,2-propanediol (1,2-PDO). Here we present a novel metabolic engineering approach to produce 1,2-PDO from glycerol in S. cerevisiae. Apart from implementing the heterologous methylglyoxal (MG) pathway for 1,2-PDO formation from dihydroxyacetone phosphate (DHAP) and expressing a heterologous glycerol facilitator, the employed genetic modifications included the replacement of the native FAD-dependent glycerol catabolic pathway by the 'DHA pathway' for delivery of cytosolic NADH and the reduction of triosephosphate isomerase (TPI) activity for increased precursor (DHAP) supply. The choice of the medium had a crucial impact on both the strength of the metabolic switch towards fermentation in general (as indicated by the production of ethanol and 1,2-PDO) and on the ratio at which these two fermentation products were formed. For example, virtually no 1,2-PDO but only ethanol was formed in synthetic glycerol medium with urea as the nitrogen source. When nutrient-limited complex YG medium was used, significant amounts of 1,2-PDO were formed and it became obvious that the concerted supply of NADH and DHAP are essential for boosting 1,2-PDO production. Additionally, optimizing the flux into the MG pathway improved 1,2-PDO formation at the expense of ethanol. Cultivation of the best-performing strain in YG medium and a controlled bioreactor set-up resulted in a maximum titer of > 4gL -1 1,2-PDO which, to the best of our knowledge, has been the highest titer of 1,2-PDO obtained in yeast so far. Surprisingly, significant 1,2-PDO production was also obtained in synthetic glycerol medium after changing the nitrogen source towards ammonium sulfate and adding a buffer. Copyright © 2017 International Metabolic

29 CFR 36.500 - Employment.

Science.gov (United States)

2010-07-01

...) Application. The provisions of §§ 36.500 through 36.550 apply to: (1) Recruitment, advertising, and the..., leave for persons of either sex to care for children or dependents, or any other leave; (7) Fringe...

Determination of Volatile Organic Compounds (VOCs from Wrapping Films and Wrapped PDO Italian Cheeses by Using HS-SPME and GC/MS

Directory of Open Access Journals (Sweden)

Sara Panseri

2014-06-01

Full Text Available Nowadays food wrapping assures attractive presentation and simplifies self-service shopping. Polyvinylchloride (PVC- and polyethylene (PE-based cling-films are widely used worldwide for wrapping cheeses. For this purpose, films used in retail possess suitable technical properties such as clinginess and unrolling capacity, that are achieved by using specific plasticizers during their manufacturing process. In the present study, the main VOCs of three cling-films (either PVC-based or PE-based for retail use were characterized by means of Solid-Phase Micro-Extraction and GC/MS. In addition, the effects of cling film type and contact time on the migration of VOCs from the films to four different PDO Italian cheeses during cold storage under light or dark were also investigated. Among the VOCs isolated from cling-films, PVC released 2-ethylhexanol and triacetin. These compounds can likely be considered as a “non-intentionally added substance”. These same compounds were also detected in cheeses wrapped in PVC films with the highest concentration found after 20 days storage. The PE cling-film was shown to possess a simpler VOC profile, lacking some molecules peculiar to PVC films. The same conclusions can be drawn for cheeses wrapped in the PE cling-film. Other VOCs found in wrapped cheeses were likely to have been released either by direct transfer from the materials used for the manufacture of cling-films or from contamination of the films. Overall, HS-SPME is shown to be a rapid and solvent free technique to screen the VOCs profile of cling-films, and to detect VOCs migration from cling-films to cheese under real retail storage conditions.

Quality uncertainty and allocation of decision rights in the European protected designation of origin

Directory of Open Access Journals (Sweden)

Gaetano Martino

2013-06-01

Full Text Available The paper considers some issue in the governance of the European Protected Designation of Origin (PDO. The PDO systems are the outcomes of both farmers and consumers expectations and connect the valorisation of the agricultural and rural resources of given territories to the quality of typical products. A critical point in the governance of the PDO systems is represented by the connection between the quality strategies and the uncertainty. The paper argues that the PDO systems can be thought of as strictly coordinated subsystems in which the ex post governance play a critical role in coping with quality uncertainty. The study suggests that the society's inducements given raise to complex organizational systems in which the allocation of decision rights to PDO collective organizations play a major role. The empirical analysis is carried out by examining ten Italian PDO systems in order to identify the decision rights allocated.

31 CFR 500.535 - Exchange of certain securities.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Exchange of certain securities. 500.535 Section 500.535 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... securities from blocked accounts: (1) Exchange of certificates necessitated by reason of changes in corporate...

Polar-Orbiting Satellite (POES) Images

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Visible and Infrared satellite imagery taken from camera systems or radiometer instruments on satellites in orbit around the poles. Satellite campaigns include...

The Eccentric Satellites Problem: Comparing Milky Way Satellite Orbital Properties to Simulation Results

Science.gov (United States)

Haji, Umran; Pryor, Carlton; Applebaum, Elaad; Brooks, Alyson

2018-01-01

We compare the orbital properties of the satellite galaxies of the Milky Way to those of satellites found in simulated Milky Way-like systems as a means of testing cosmological simulations of galaxy formation. The particular problem that we are investigating is a discrepancy in the distribution of orbital eccentricities. Previous studies of Milky Way-mass systems analyzed in a semi-analytic ΛCDM cosmological model have found that the satellites tend to have significantly larger fractions of their kinetic energy invested in radial motion with respect to their central galaxy than do the real-world Milky Way satellites. We analyze several high-resolution ("zoom-in") hydrodynamical simulations of Milky Way-mass galaxies and their associated satellite systems to investigate why previous works found Milky Way-like systems to be rare. We find a possible relationship between a quiescent galactic assembly history and a distribution of satellite kinematics resembling that of the Milky Way. This project has been supported by funding from National Science Foundation grant PHY-1560077.

12 CFR 500.30 - General statement concerning procedures and forms.

Science.gov (United States)

2010-01-01

... 12 Banks and Banking 5 2010-01-01 2010-01-01 false General statement concerning procedures and forms. 500.30 Section 500.30 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS Procedures § 500.30 General statement concerning procedures and forms...

Satellite switched FDMA advanced communication technology satellite program

Science.gov (United States)

Atwood, S.; Higton, G. H.; Wood, K.; Kline, A.; Furiga, A.; Rausch, M.; Jan, Y.

1982-01-01

The satellite switched frequency division multiple access system provided a detailed system architecture that supports a point to point communication system for long haul voice, video and data traffic between small Earth terminals at Ka band frequencies at 30/20 GHz. A detailed system design is presented for the space segment, small terminal/trunking segment at network control segment for domestic traffic model A or B, each totaling 3.8 Gb/s of small terminal traffic and 6.2 Gb/s trunk traffic. The small terminal traffic (3.8 Gb/s) is emphasized, for the satellite router portion of the system design, which is a composite of thousands of Earth stations with digital traffic ranging from a single 32 Kb/s CVSD voice channel to thousands of channels containing voice, video and data with a data rate as high as 33 Mb/s. The system design concept presented, effectively optimizes a unique frequency and channelization plan for both traffic models A and B with minimum reorganization of the satellite payload transponder subsystem hardware design. The unique zoning concept allows multiple beam antennas while maximizing multiple carrier frequency reuse. Detailed hardware design estimates for an FDMA router (part of the satellite transponder subsystem) indicate a weight and dc power budget of 353 lbs, 195 watts for traffic model A and 498 lbs, 244 watts for traffic model B.

Partnership via Satellite.

Science.gov (United States)

Powell, Marie Clare

1980-01-01

Segments of the 1980 National Catholic Educational Association (NCEA) conference were to be telecast nationally by satellite. The author briefly explains the satellite transmission process and advises Catholic educators on how to pick up the broadcast through their local cable television system. (SJL)

Integrating plant and animal biology for the search of novel DNA damage biomarkers

Czech Academy of Sciences Publication Activity Database

Nikitaki, Z.; Holá, Marcela; Donà, M.; Pavlopoulou, A.; Michalopoulos, I.; Angelis, Karel; Georgakilas, A. G.; Macovei, I.; Balestrazzi, A.

2018-01-01

Roč. 775, JAN-MAR (2018), s. 21-38 ISSN 1383-5742 R&D Projects: GA ČR GA16-01137S Institutional support: RVO:61389030 Keywords : DNA damage response * Ionizing radiation * Radiation exposure monitoring * Radiotolerance * Ultraviolet radiation Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Genetics and heredity (medical genetics to be 3) Impact factor: 5.500, year: 2016

29 CFR 500.269 - Stay pending decision of the Secretary.

Science.gov (United States)

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Stay pending decision of the Secretary. 500.269 Section 500.269 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR... Vacation of Order of Administrative Law Judge § 500.269 Stay pending decision of the Secretary. (a) The...

41 CFR 101-27.500 - Scope and applicability of subpart.

Science.gov (United States)

2010-07-01

... MANAGEMENT 27.5-Return of GSA Stock Items § 101-27.500 Scope and applicability of subpart. This subpart sets... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Scope and applicability of subpart. 101-27.500 Section 101-27.500 Public Contracts and Property Management Federal Property...

Spectrum and power allocation in cognitive multi-beam satellite communications with flexible satellite payloads

Science.gov (United States)

Liu, Zhihui; Wang, Haitao; Dong, Tao; Yin, Jie; Zhang, Tingting; Guo, Hui; Li, Dequan

2018-02-01

In this paper, the cognitive multi-beam satellite system, i.e., two satellite networks coexist through underlay spectrum sharing, is studied, and the power and spectrum allocation method is employed for interference control and throughput maximization. Specifically, the multi-beam satellite with flexible payload reuses the authorized spectrum of the primary satellite, adjusting its transmission band as well as power for each beam to limit its interference on the primary satellite below the prescribed threshold and maximize its own achievable rate. This power and spectrum allocation problem is formulated as a mixed nonconvex programming. For effective solving, we first introduce the concept of signal to leakage plus noise ratio (SLNR) to decouple multiple transmit power variables in the both objective and constraint, and then propose a heuristic algorithm to assign spectrum sub-bands. After that, a stepwise plus slice-wise algorithm is proposed to implement the discrete power allocation. Finally, simulation results show that adopting cognitive technology can improve spectrum efficiency of the satellite communication.

A Multi-Criteria Approach to Assessing PDOs/PGIs: An Italian Pilot Study

Directory of Open Access Journals (Sweden)

Francesca Galli

2011-12-01

Full Text Available  The paper contribute to the assessment of PDO/PGI schemes by building an approach to measuring the actual performance of the PDO/PGI products in relation to the objectives of the European Regulation 510/2006. This is done through a multi-criteria ex-post analysis that compares the performance of different PDO/PGI products with respect to multiple criteria. The research presented, based on a small sample of Pdo cheese products in Italy, can be considered a pilot study that develops a comparative evaluation of the performance of PDO cheeses: i relative to the different objectives of the schemes, ii by using a set of suitable and informative indicators that can help to evaluate the effectiveness of a wide number of PDOs/PGIs with respect to the regulation’s objectives, and iii by considering that different actors involved in PDO/PGI schemes may have different priorities with regard to the importance of different objectives in evaluating the performance of schemes.

Polyhydroxyalkanoates (PHA) production from fermented crude glycerol: Study on the conversion of 1,3-propanediol to PHA in mixed microbial consortia

DEFF Research Database (Denmark)

Burniol Figols, Anna; Varrone, Cristiano; Daugaard, Anders Egede

2018-01-01

Crude glycerol, a by-product from the biodiesel industry, can be converted by mixed microbial consortia into 1,3-propanediol (1,3-PDO) and volatile fatty acids. In this study, further conversion of these main products into polyhydroxyalkanoates (PHA) was investigated with the focus on 1,3-PDO. Two...... different approaches for the enrichment of PHA accumulating microbial consortia using an aerobic dynamic feeding strategy were applied. With the first approach, where nitrogen was present during the whole cycle, no net production of PHA from 1,3-PDO was observed in the fermented effluent, not even...... the storage response. Nitrogen was still supplied during the famine phase. With the latter strategy, a net production of PHA from 1,3-PDO was observed at a yield of 0.24 Cmol PHA/Cmol 1,3-PDO. The overall yield from the fermented effluent was 0.42 Cmol PHA/Cmol substrate. Overall, the PHA yield from 1,3-PDO...

A modular metabolic engineering approach for the production of 1,2-propanediol from glycerol by Saccharomyces cerevisiae

DEFF Research Database (Denmark)

Islam, Zia-Ul; Klein, Mathias; Aßkamp, Maximilian R.

2017-01-01

Compared to sugars, a major advantage of using glycerol as a feedstock for industrial bioprocesses is the fact that this molecule is more reduced than sugars. A compound whose biotechnological production might greatly profit from the substrate's higher reducing power is 1,2-propanediol (1,2-PDO...... as the nitrogen source. When nutrient-limited complex YG medium was used, significant amounts of 1,2-PDO were formed and it became obvious that the concerted supply of NADH and DHAP are essential for boosting 1,2-PDO production. Additionally, optimizing the flux into the MG pathway improved 1,2-PDO formation...... on both the strength of the metabolic switch towards fermentation in general (as indicated by the production of ethanol and 1,2-PDO) and on the ratio at which these two fermentation products were formed. For example, virtually no 1,2-PDO but only ethanol was formed in synthetic glycerol medium with urea...

29 CFR 500.232 - Consent findings and order.

Science.gov (United States)

2010-07-01

... Administrative Law Judge; and (4) A waiver of any right to challenge or contest the validity of the findings and... 29 Labor 3 2010-07-01 2010-07-01 false Consent findings and order. 500.232 Section 500.232 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS MIGRANT AND...

Interim Service ISDN Satellite (ISIS) hardware experiment development for advanced ISDN satellite designs and experiments

Science.gov (United States)

Pepin, Gerard R.

1992-01-01

The Interim Service Integrated Service Digital Network (ISDN) Satellite (ISIS) Hardware Experiment Development for Advanced Satellite Designs describes the development of the ISDN Satellite Terminal Adapter (ISTA) capable of translating ISDN protocol traffic into Time Division Multiple Access (TDMA) signals for use by a communications satellite. The ISTA connects the Type 1 Network Termination (NT1) via the U-interface on the line termination side of the CPE to the RS-499 interface for satellite uplink. The same ISTA converts in the opposite direction the RS-499 to U-interface data with a simple switch setting.

Interim Service ISDN Satellite (ISIS) hardware experiment design for advanced ISDN satellite design and experiments

Science.gov (United States)

Pepin, Gerard R.

1992-01-01

The Interim Service Integrated Services Digital Network (ISDN) Satellite (ISIS) Hardware Experiment Design for Advanced Satellite Designs describes the design of the ISDN Satellite Terminal Adapter (ISTA) capable of translating ISDN protocol traffic into time division multiple access (TDMA) signals for use by a communications satellite. The ISTA connects the Type 1 Network Termination (NT1) via the U-interface on the line termination side of the CPE to the V.35 interface for satellite uplink. The same ISTA converts in the opposite direction the V.35 to U-interface data with a simple switch setting.

BURNER RIG TESTING OF A500 C/SiC

Science.gov (United States)

2018-03-17

AFRL-RX-WP-TR-2018-0071 BURNER RIG TESTING OF A500® C /SiC Larry P. Zawada Universal Technology Corporation Jennifer Pierce UDRI...TITLE AND SUBTITLE BURNER RIG TESTING OF A500® C /SiC 5a. CONTRACT NUMBER In-House 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 62102F 6...test program characterized the durability behavior of A500® C /SiC ceramic matrix composite material at room and elevated temperature. Specimens were

The Skeletal Muscle Satellite Cell

Science.gov (United States)

2011-01-01

The skeletal muscle satellite cell was first described and named based on its anatomic location between the myofiber plasma and basement membranes. In 1961, two independent studies by Alexander Mauro and Bernard Katz provided the first electron microscopic descriptions of satellite cells in frog and rat muscles. These cells were soon detected in other vertebrates and acquired candidacy as the source of myogenic cells needed for myofiber growth and repair throughout life. Cultures of isolated myofibers and, subsequently, transplantation of single myofibers demonstrated that satellite cells were myogenic progenitors. More recently, satellite cells were redefined as myogenic stem cells given their ability to self-renew in addition to producing differentiated progeny. Identification of distinctively expressed molecular markers, in particular Pax7, has facilitated detection of satellite cells using light microscopy. Notwithstanding the remarkable progress made since the discovery of satellite cells, researchers have looked for alternative cells with myogenic capacity that can potentially be used for whole body cell-based therapy of skeletal muscle. Yet, new studies show that inducible ablation of satellite cells in adult muscle impairs myofiber regeneration. Thus, on the 50th anniversary since its discovery, the satellite cell’s indispensable role in muscle repair has been reaffirmed. PMID:22147605

DNA detection on ultrahigh-density optical fiber-based nanoarrays.

Science.gov (United States)

Tam, Jenny M; Song, Linan; Walt, David R

2009-04-15

Nanoarrays for DNA detection were fabricated on etched nanofiber bundles based on recently developed techniques for microscale arrays. Two different-sized nanoarrays were created: one with 700 nm feature sizes and a 1 microm center-to-center pitch (approximately 1x10(6) array elements/mm(2)) and one with 300 nm feature sizes and a 500 nm center-to-center pitch (4.6x10(6) array elements/mm(2)). A random, multiplexed array composed of oligonucleotide-functionalized nanospheres was constructed and used for parallel detection and analysis of fluorescently labeled DNA targets. We have used these arrays to detect a variety of target sequences including Bacillus thuringiensis kurstaki and vaccina virus sequences, two potential biowarfare agents, as well as interleukin-2 sequences, an immune system modulator that has been used for the diagnosis of HIV.

Analysis of DNA restriction fragments greater than 5.7 Mb in size from the centromeric region of human chromosomes.

Science.gov (United States)

Arn, P H; Li, X; Smith, C; Hsu, M; Schwartz, D C; Jabs, E W

1991-01-01

Pulsed electrophoresis was used to study the organization of the human centromeric region. Genomic DNA was digested with rare-cutting enzymes. DNA fragments from 0.2 to greater than 5.7 Mb were separated by electrophoresis and hybridized with alphoid and simple DNA repeats. Rare-cutting enzymes (Mlu I, Nar I, Not I, Nru I, Sal I, Sfi I, Sst II) demonstrated fewer restriction sites at centromeric regions than elsewhere in the genome. The enzyme Not I had the fewest restriction sites at centromeric regions. As much as 70% of these sequences from the centromeric region are present in Not I DNA fragments greater than 5.7 and estimated to be as large as 10 Mb in size. Other repetitive sequences such as short interspersed repeated segments (SINEs), long interspersed repeated segments (LINEs), ribosomal DNA, and mini-satellite DNA that are not enriched at the centromeric region, are not enriched in Not I fragments of greater than 5.7 Mb in size.

23 CFR 500.106 - PMS.

Science.gov (United States)

2010-04-01

... the “AASHTO Guidelines for Pavement Management Systems.” 1 1 AASHTO Guidelines for Pavement Management... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRANSPORTATION INFRASTRUCTURE MANAGEMENT MANAGEMENT AND MONITORING SYSTEMS Management Systems § 500.106 PMS. An effective PMS for Federal-aid highways is a systematic...

23 CFR 500.101 - Purpose.

Science.gov (United States)

2010-04-01

... HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRANSPORTATION INFRASTRUCTURE MANAGEMENT MANAGEMENT AND MONITORING SYSTEMS Management Systems § 500.101 Purpose. The purpose of this part is to implement... highway pavement of Federal-aid highways (PMS), bridges on and off Federal-aid highways (BMS), highway...

Quantification of differential gene expression by multiplexed targeted resequencing of cDNA

Science.gov (United States)

Arts, Peer; van der Raadt, Jori; van Gestel, Sebastianus H.C.; Steehouwer, Marloes; Shendure, Jay; Hoischen, Alexander; Albers, Cornelis A.

2017-01-01

Whole-transcriptome or RNA sequencing (RNA-Seq) is a powerful and versatile tool for functional analysis of different types of RNA molecules, but sample reagent and sequencing cost can be prohibitive for hypothesis-driven studies where the aim is to quantify differential expression of a limited number of genes. Here we present an approach for quantification of differential mRNA expression by targeted resequencing of complementary DNA using single-molecule molecular inversion probes (cDNA-smMIPs) that enable highly multiplexed resequencing of cDNA target regions of ∼100 nucleotides and counting of individual molecules. We show that accurate estimates of differential expression can be obtained from molecule counts for hundreds of smMIPs per reaction and that smMIPs are also suitable for quantification of relative gene expression and allele-specific expression. Compared with low-coverage RNA-Seq and a hybridization-based targeted RNA-Seq method, cDNA-smMIPs are a cost-effective high-throughput tool for hypothesis-driven expression analysis in large numbers of genes (10 to 500) and samples (hundreds to thousands). PMID:28474677

Electronic Health Records Place 1st at Indy 500

Science.gov (United States)

... Navigation Bar Home Current Issue Past Issues EHR Electronic Health Records Place 1st at Indy 500 Past ... last May's Indy 500 had thousands of personal Electronic Health Records on hand for those attending—and ...

Characteristics of palindromic sequences in DNA of the sea urchin Stronglyocentrotus intermedius

International Nuclear Information System (INIS)

Brykov, V.A.; Kukhlevskii, A.D.

1986-01-01

The fraction of palindromic sequences in the nuclear DNA of the sea urchin S. intermedius was characterized. Using chromatography on hydroxyapatite and treatment with S1 nuclease, it was shown that the fraction of palindromic sequences more than doubles when the sodium concentration in solution is increased or the temperature of reassociation is lowered. The increase is due to the involvement of inverted repeats in reassociation, which are characterized by a substantial nonhomologous character and/or the presence of an extended intervening DNA sequence. It was found by the method of reassociation of a nicked palindrome fraction with an excess of total homologous DNA that most of the inverted repeats in the sea urchin genome are unique sequences. The complexity of the palindrome fraction was estimated at 8.2 x 10 7 nucleotide pairs, and the number of palindromes per haploid genome ∼ 500,000

Telelibrary: Library Services via Satellite.

Science.gov (United States)

Liu, Rosa

1979-01-01

Investigates the provision of library services via satellite, explains briefly the operation and advantages of communication satellites, and discusses the various telecommunications equipment and services which, when coupled with satellite transmission, will enhance library activities. Demand trend projections for telecommunications services…

Periosteal Distraction Osteogenesis: An Effective Method for Bone Regeneration

Directory of Open Access Journals (Sweden)

Danyang Zhao

2016-01-01

Full Text Available The treatment of bone defects is challenging and controversial. As a new technology, periosteal distraction osteogenesis (PDO uses the osteogenicity of periosteum, which creates an artificial space between the bone surface and periosteum to generate new bone by gradually expanding the periosteum with no need for corticotomy. Using the newly formed bone of PDO to treat bone defects is effective, which can not only avoid the occurrence of immune-related complications, but also solve the problem of insufficient donor. This review elucidates the availability of PDO in the aspects of mechanisms, devices, strategies, and measures. Moreover, we also focus on the future prospects of PDO and hope that PDO will be applied to the clinical treatment of bone defects in the future.

Effect of tropical cyclones on the stratosphere–troposphere exchange observed using satellite observations over the north Indian Ocean

Directory of Open Access Journals (Sweden)

M. Venkat Ratnam

2016-07-01

Full Text Available Tropical cyclones play an important role in modifying the tropopause structure and dynamics as well as stratosphere–troposphere exchange (STE processes in the upper troposphere and lower stratosphere (UTLS region. In the present study, the impact of cyclones that occurred over the north Indian Ocean during 2007–2013 on the STE processes is quantified using satellite observations. Tropopause characteristics during cyclones are obtained from the Global Positioning System (GPS radio occultation (RO measurements, and ozone and water vapour concentrations in the UTLS region are obtained from Aura Microwave Limb Sounder (MLS satellite observations. The effect of cyclones on the tropopause parameters is observed to be more prominent within 500 km of the centre of the tropical cyclone. In our earlier study, we observed a decrease (increase in the tropopause altitude (temperature up to 0.6 km (3 K, and the convective outflow level increased up to 2 km. This change leads to a total increase in the tropical tropopause layer (TTL thickness of 3 km within 500 km of the centre of cyclone. Interestingly, an enhancement in the ozone mixing ratio in the upper troposphere is clearly noticed within 500 km from the cyclone centre, whereas the enhancement in the water vapour in the lower stratosphere is more significant on the south-east side, extending from 500 to 1000 km away from the cyclone centre. The cross-tropopause mass flux for different intensities of cyclones is estimated and it is found that the mean flux from the stratosphere to the troposphere for cyclonic storms is 0.05 ± 0.29 × 10−3 kg m−2, and for very severe cyclonic storms it is 0.5 ± 1.07 × 10−3 kg m−2. More downward flux is noticed on the north-west and south-west side of the cyclone centre. These results indicate that the cyclones have significant impact in effecting the tropopause structure, ozone and water vapour budget, and

Spectroscopic (UV/VIS, Raman) and Electrophoresis Study of Cytosine-Guanine Oligonucleotide DNA Influenced by Magnetic Field.

Science.gov (United States)

Banihashemian, Seyedeh Maryam; Periasamy, Vengadesh; Boon Tong, Goh; Abdul Rahman, Saadah

2016-01-01

Studying the effect of a magnetic field on oligonucleotide DNA can provide a novel DNA manipulation technique for potential application in bioengineering and medicine. In this work, the optical and electrochemical response of a 100 bases oligonucleotides DNA, cytosine-guanine (CG100), is investigated via exposure to different magnetic fields (250, 500, 750, and 1000 mT). As a result of the optical response of CG100 to the magnetic field, the ultra-violet-visible spectrum indicated a slight variation in the band gap of CG100 of about 0.3 eV. Raman spectroscopy showed a significant deviation in hydrogen and phosphate bonds' vibration after exposure to the magnetic field. Oligonucleotide DNA mobility was investigated in the external electric field using the gel electrophoresis technique, which revealed a small decrease in the migration of CG100 after exposure to the magnetic field.

Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

Directory of Open Access Journals (Sweden)

Moulick A

2017-02-01

Full Text Available Amitava Moulick,1,2 Vedran Milosavljevic,1,2 Jana Vlachova,1,2 Robert Podgajny,3 David Hynek,1,2 Pavel Kopel,1,2 Vojtech Adam1,2 1Department of Chemistry and Biochemistry, Mendel University, 2Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; 3Faculty of Chemistry, Jagiellonian University, Krakow, Poland Abstract: CdTe/ZnSe core/shell quantum dot (QD, one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3 and normal (PNT1A cells (detection limit of 500 pM of DNA, which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments. Keywords: nanoparticles, nucleobases, biosensor, fluorescence, mutation

A Tandemly Arranged Pattern of Two 5S rDNA Arrays in Amolops mantzorum (Anura, Ranidae).

Science.gov (United States)

Liu, Ting; Song, Menghuan; Xia, Yun; Zeng, Xiaomao

2017-01-01

In an attempt to extend the knowledge of the 5S rDNA organization in anurans, the 5S rDNA sequences of Amolops mantzorum were isolated, characterized, and mapped by FISH. Two forms of 5S rDNA, type I (209 bp) and type II (about 870 bp), were found in specimens investigated from various populations. Both of them contained a 118-bp coding sequence, readily differentiated by their non-transcribed spacer (NTS) sizes and compositions. Four probes (the 5S rDNA coding sequences, the type I NTS, the type II NTS, and the entire type II 5S rDNA sequences) were respectively labeled with TAMRA or digoxigenin to hybridize with mitotic chromosomes for samples of all localities. It turned out that all probes showed the same signals that appeared in every centromeric region and in the telomeric regions of chromosome 5, without differences within or between populations. Obviously, both type I and type II of the 5S rDNA arrays arranged in tandem, which was contrasting with other frogs or fishes recorded to date. More interestingly, all the probes detected centromeric regions in all karyotypes, suggesting the presence of a satellite DNA family derived from 5S rDNA. © 2017 S. Karger AG, Basel.

23 CFR 500.105 - Requirements.

Science.gov (United States)

2010-04-01

... HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION TRANSPORTATION INFRASTRUCTURE MANAGEMENT MANAGEMENT AND MONITORING SYSTEMS Management Systems § 500.105 Requirements. (a) The metropolitan transportation planning process (23 U.S.C. 134 and 49 U.S.C. 5303-5005) in TMAs shall include a CMS that meets the...

Activities of Canadian Satellite Communications, Inc.

Science.gov (United States)

1992-12-01

Canadian Satellite Communications (Cancom) has as its core business the provision of television and radio signals to cable systems in Canada, with the objective of making affordable broadcast signals available to remote and/or small communities. Cancom also provides direct-to-home services to backyard receiving dishes, as well as satellite digital data business communications services, satellite business television, and satellite network services. Its business communication services range from satellite links for big-city businesses with small branch operations located far from major centers, to a mobile messaging and tracking system for the trucking industry. Revenues in 1992 totalled $48,212,000 and net income was just over $7 million. Cancom bought 10 percent interest in Leosat Corp. of Washington, DC, who are seeking approval to operate a position locator network from low-orbit satellites. Cancom has also become a partner in SovCan Star Satellite Communications Inc., which will build an international satellite system in partnership with Russia. The first satellite in this east-west business network will be placed in a Russian orbital slot over the Atlantic by 1996, and a second satellite will follow for the Pacific region. This annual report of Cancom's activities for 1992 includes financial statements and a six year financial review.

Security Concepts for Satellite Links

Science.gov (United States)

Tobehn, C.; Penné, B.; Rathje, R.; Weigl, A.; Gorecki, Ch.; Michalik, H.

2008-08-01

The high costs to develop, launch and maintain a satellite network makes protecting the assets imperative. Attacks may be passive such as eavesdropping on the payload data. More serious threat are active attacks that try to gain control of the satellite, which may lead to the total lost of the satellite asset. To counter these threats, new satellite and ground systems are using cryptographic technologies to provide a range of services: confidentiality, entity & message authentication, and data integrity. Additionally, key management cryptographic services are required to support these services. This paper describes the key points of current satellite control and operations, that are authentication of the access to the satellite TMTC link and encryption of security relevant TM/TC data. For payload data management the key points are multi-user ground station access and high data rates both requiring frequent updates and uploads of keys with the corresponding key management methods. For secure satellite management authentication & key negotiation algorithms as HMAC-RIPEMD160, EC- DSA and EC-DH are used. Encryption of data uses algorithms as IDEA, AES, Triple-DES, or other. A channel coding and encryption unit for payload data provides download data rates up to Nx250 Mbps. The presented concepts are based on our experience and heritage of the security systems for all German MOD satellite projects (SATCOMBw2, SAR-Lupe multi- satellite system and German-French SAR-Lupe-Helios- II systems inter-operability) as well as for further international (KOMPSAT-II Payload data link system) and ESA activities (TMTC security and GMES).

JPSS Preparations at the Satellite Proving Ground for Marine, Precipitation, and Satellite Analysis

Science.gov (United States)

Folmer, M. J.; Berndt, E.; Clark, J.; Orrison, A.; Kibler, J.; Sienkiewicz, J. M.; Nelson, J. A., Jr.; Goldberg, M.

2016-12-01

The National Oceanic and Atmospheric Administration (NOAA) Satellite Proving Ground (PG) for Marine, Precipitation, and Satellite Analysis (MPS) has been demonstrating and evaluating Suomi National Polar-orbiting Partnership (S-NPP) products along with other polar-orbiting satellite platforms in preparation for the Joint Polar Satellite System - 1 (JPSS-1) launch in March 2017. The first S-NPP imagery was made available to the MPS PG during the evolution of Hurricane Sandy in October 2012 and has since been popular in operations. Since this event the MPS PG Satellite Liaison has been working with forecasters on ways to integrate single-channel and multispectral imagery from the Visible Infrared Imaging Radiometer Suite (VIIRS), the Moderate Resolution Imaging Spectroradiometer (MODIS), and the Advanced Very High Resolution Radiometer (AVHRR)into operations to complement numerical weather prediction and geostationary satellite savvy National Weather Service (NWS) National Centers. Additional unique products have been introduced to operations to address specific forecast challenges, including the Cooperative Institute for Research in the Atmosphere (CIRA) Layered Precipitable Water, the National Environmental Satellite, Data, and Information Service (NESDIS) Snowfall Rate product, NOAA Unique Combined Atmospheric Processing System (NUCAPS) Soundings, ozone products from the Atmospheric Infrared Sounder (AIRS), Cross-track Infrared Sounder/Advanced Technology Microwave Sounder (CrIS/ATMS), and Infrared Atmospheric Sounding Interferometer (IASI). In addition, new satellite domains have been created to provide forecasters at the NWS Ocean Prediction Center and Weather Prediction Center with better quality imagery at high latitudes. This has led to research projects that are addressing forecast challenges such as tropical to extratropical transition and explosive cyclogenesis. This presentation will provide examples of how the MPS PG has been introducing and integrating

Glycerol and environmental factors: effects on 1,3-propanediol production and NAD(+) regeneration in Lactobacillus panis PM1.

Science.gov (United States)

Kang, T S; Korber, D R; Tanaka, T

2013-10-01

This study was conducted to understand the influences of fermentation factors in NADH recycling and mechanisms of 1,3-propanediol (1,3-PDO) production in Lactobacillus panis PM1. We conducted metabolite analyses, qRT-PCR of the glycerol reductive pathway [glycerol dehydratase (DhaB) and 1,3-PDO dehydrogenase (DhaT)] and DhaT activity assays at different pH, temperature and initial glycerol concentrations. The supplementation of 150 mmol l(-1) glycerol caused a shift in NADH flux from ethanol to 1,3-PDO production, whereas 300 mol l(-1) glycerol negatively affected the regeneration of NAD(+) via 1,3-PDO production. This retardation decreased transcription levels and specific activities of DhaT. The decreased DhaT activity eventually caused the shutdown of 1,3-PDO production. Temperature and pH did not significantly affect the specific activity of DhaT, whereas expression of genes for DhaB and DhaT was activated under acidic conditions. Moreover, fresh glucose addition after its depletion could not restart the glycerol reduction, but increased ethanol production. Those environmental factors affect 1,3-PDO production in different ways through changing the expression level of enzymes and shifting the NAD(+) regeneration pathways. Our findings elucidated a key element to optimize 1,3-PDO production by Lact. panis PM1, which potentially improves 1,3-PDO manufacturing efficiencies. © 2013 The Society for Applied Microbiology.

Satellite services system overview

Science.gov (United States)

Rysavy, G.

1982-01-01

The benefits of a satellite services system and the basic needs of the Space Transportation System to have improved satellite service capability are identified. Specific required servicing equipment are discussed in terms of their technology development status and their operative functions. Concepts include maneuverable television systems, extravehicular maneuvering unit, orbiter exterior lighting, satellite holding and positioning aid, fluid transfer equipment, end effectors for the remote manipulator system, teleoperator maneuvering system, and hand and power tools.

Mobility management in satellite networks

Science.gov (United States)

Johanson, Gary A.

1995-01-01

This paper addresses the methods used or proposed for use in multi-beam and/or multi-satellite networks designed to provide Mobile Satellite Services (MSS). Specific topics include beam crossover in the North American Mobile Satellite (MSAT) system as well as registration and live call hand-off for a multi-regional geosynchronous (GEO) satellite based system and a global coverage Low Earth Orbiting (LEO) system. In the MSAT system, the individual satellite beams cover very large geographic areas so the need for live call hand-off was not anticipated. This paper discusses the methods used to keep track of the beam location of the users so that incoming call announcements or other messages may be directed to them. Proposed new GEO systems with large numbers of beams will provide much smaller geographic coverage in individual beams and thus the need arises to keep track of the user's location as well as to provide live call hand-off as the user traverses from beam to beam. This situation also occurs in proposed LEO systems where the problems are worsened by the need for satellite to satellite hand-off as well as beam to beam hand-off within a single satellite. The paper discusses methods to accomplish these handoffs and proposes system architectures to address the various hand-off scenarios.

Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol

Directory of Open Access Journals (Sweden)

Li Han

2013-01-01

Full Text Available Abstract Background The modern society primarily relies on petroleum and natural gas for the production of fuels and chemicals. One of the major commodity chemicals 1,2-propanediol (1,2-PDO, which has an annual production of more than 0.5 million tons in the United States, is currently produced by chemical processes from petroleum derived propylene oxide, which is energy intensive and not sustainable. In this study, we sought to achieve photosynthetic production of 1,2-PDO from CO2 using a genetically engineered cyanobacterium Synechococcus elongatus PCC 7942. Compared to the previously reported biological 1,2-PDO production processes which used sugar or glycerol as the substrates, direct chemical production from CO2 in photosynthetic organisms recycles the atmospheric CO2 and will not compete with food crops for arable land. Results In this study, we reported photosynthetic production of 1,2-PDO from CO2 using a genetically engineered cyanobacterium Synechococcus elongatus PCC 7942. Introduction of the genes encoding methylglyoxal synthase (mgsA, glycerol dehydrogenase (gldA, and aldehyde reductase (yqhD resulted in the production of ~22mg/L 1,2-PDO from CO2. However, a comparable amount of the pathway intermediate acetol was also produced, especially during the stationary phase. The production of 1,2-PDO requires a robust input of reducing equivalents from cellular metabolism. To take advantage of cyanobacteria’s NADPH pool, the synthetic pathway of 1,2-PDO was engineered to be NADPH-dependent by exploiting the NADPH-specific secondary alcohol dehydrogenases which have not been reported for 1,2-PDO production previously. This optimization strategy resulted in the production of ~150mg/L 1,2-PDO and minimized the accumulation of the incomplete reduction product, acetol. Conclusion This work demonstrated that cyanobacteria can be engineered as a catalyst for the photosynthetic conversion of CO2 to 1,2-PDO. This work also characterized two NADPH

31 CFR 28.500 - Employment.

Science.gov (United States)

2010-07-01

... benefits of, or be subjected to discrimination in employment, or recruitment, consideration, or selection... §§ 28.500 through 28.550 apply to: (1) Recruitment, advertising, and the process of application for...-sponsored activities, including social or recreational programs; and (10) Any other term, condition, or...

GPS Satellite Simulation Facility

Data.gov (United States)

Federal Laboratory Consortium — The GPS satellite simulation facility consists of a GPS satellite simulator controlled by either a Silicon Graphics Origin 2000 or PC depending upon unit under test...

Validation of Earth atmosphere models using solar EUV observations from the CORONAS and PROBA2 satellites in occultation mode

Science.gov (United States)

Slemzin, Vladimir; Ulyanov, Artyom; Gaikovich, Konstantin; Kuzin, Sergey; Pertsov, Andrey; Berghmans, David; Dominique, Marie

2016-02-01

Aims: Knowledge of properties of the Earth's upper atmosphere is important for predicting the lifetime of low-orbit spacecraft as well as for planning operation of space instruments whose data may be distorted by atmospheric effects. The accuracy of the models commonly used for simulating the structure of the atmosphere is limited by the scarcity of the observations they are based on, so improvement of these models requires validation under different atmospheric conditions. Measurements of the absorption of the solar extreme ultraviolet (EUV) radiation in the upper atmosphere below 500 km by instruments operating on low-Earth orbits (LEO) satellites provide efficient means for such validation as well as for continuous monitoring of the upper atmosphere and for studying its response to the solar and geomagnetic activity. Method: This paper presents results of measurements of the solar EUV radiation in the 17 nm wavelength band made with the SPIRIT and TESIS telescopes on board the CORONAS satellites and the SWAP telescope on board the PROBA2 satellite in the occulted parts of the satellite orbits. The transmittance profiles of the atmosphere at altitudes between 150 and 500 km were derived from different phases of solar activity during solar cycles 23 and 24 in the quiet state of the magnetosphere and during the development of a geomagnetic storm. We developed a mathematical procedure based on the Tikhonov regularization method for solution of ill-posed problems in order to retrieve extinction coefficients from the transmittance profiles. The transmittance profiles derived from the data and the retrieved extinction coefficients are compared with simulations carried out with the NRLMSISE-00 atmosphere model maintained by Naval Research Laboratory (USA) and the DTM-2013 model developed at CNES in the framework of the FP7 project ATMOP. Results: Under quiet and slightly disturbed magnetospheric conditions during high and low solar activity the extinction coefficients

DS-CDMA satellite diversity reception for personal satellite communication: Downlink performance analysis

Science.gov (United States)

DeGaudenzi, Riccardo; Giannetti, Filippo

1995-01-01

The downlink of a satellite-mobile personal communication system employing power-controlled Direct Sequence Code Division Multiple Access (DS-CDMA) and exploiting satellite-diversity is analyzed and its performance compared with a more traditional communication system utilizing single satellite reception. The analytical model developed has been thoroughly validated by means of extensive Monte Carlo computer simulations. It is shown how the capacity gain provided by diversity reception shrinks considerably in the presence of increasing traffic or in the case of light shadowing conditions. Moreover, the quantitative results tend to indicate that to combat system capacity reduction due to intra-system interference, no more than two satellites shall be active over the same region. To achieve higher system capacity, differently from terrestrial cellular systems, Multi-User Detection (MUD) techniques are likely to be required in the mobile user terminal, thus considerably increasing its complexity.

Satellite-Based Sunshine Duration for Europe

Directory of Open Access Journals (Sweden)

Bodo Ahrens

2013-06-01

Full Text Available In this study, two different methods were applied to derive daily and monthly sunshine duration based on high-resolution satellite products provided by the European Organisation for the Exploitation of Meteorological Satellites (EUMETSAT Satellite Application Facility on Climate Monitoring using data from Meteosat Second Generation (MSG SEVIRI (Spinning Enhanced Visible and Infrared Imager. The satellite products were either hourly cloud type or hourly surface incoming direct radiation. The satellite sunshine duration estimates were not found to be significantly different using the native 15-minute temporal resolution of SEVIRI. The satellite-based sunshine duration products give additional spatial information over the European continent compared with equivalent in situ-based products. An evaluation of the satellite sunshine duration by product intercomparison and against station measurements was carried out to determine their accuracy. The satellite data were found to be within ±1 h/day compared to high-quality Baseline Surface Radiation Network or surface synoptic observations (SYNOP station measurements. The satellite-based products differ more over the oceans than over land, mainly because of the treatment of fractional clouds in the cloud type-based sunshine duration product. This paper presents the methods used to derive the satellite sunshine duration products and the performance of the different retrievals. The main benefits and disadvantages compared to station-based products are also discussed.

Accelerating plant DNA barcode reference library construction using herbarium specimens: improved experimental techniques.

Science.gov (United States)

Xu, Chao; Dong, Wenpan; Shi, Shuo; Cheng, Tao; Li, Changhao; Liu, Yanlei; Wu, Ping; Wu, Hongkun; Gao, Peng; Zhou, Shiliang

2015-11-01

A well-covered reference library is crucial for successful identification of species by DNA barcoding. The biggest difficulty in building such a reference library is the lack of materials of organisms. Herbarium collections are potentially an enormous resource of materials. In this study, we demonstrate that it is likely to build such reference libraries using the reconstructed (self-primed PCR amplified) DNA from the herbarium specimens. We used 179 rosaceous specimens to test the effects of DNA reconstruction, 420 randomly sampled specimens to estimate the usable percentage and another 223 specimens of true cherries (Cerasus, Rosaceae) to test the coverage of usable specimens to the species. The barcode rbcLb (the central four-sevenths of rbcL gene) and matK was each amplified in two halves and sequenced on Roche GS 454 FLX+. DNA from the herbarium specimens was typically shorter than 300 bp. DNA reconstruction enabled amplification fragments of 400-500 bp without bringing or inducing any sequence errors. About one-third of specimens in the national herbarium of China (PE) were proven usable after DNA reconstruction. The specimens in PE cover all Chinese true cherry species and 91.5% of vascular species listed in Flora of China. It is very possible to build well-covered reference libraries for DNA barcoding of vascular species in China. As exemplified in this study, DNA reconstruction and DNA-labelled next-generation sequencing can accelerate the construction of local reference libraries. By putting the local reference libraries together, a global library for DNA barcoding becomes closer to reality. © 2015 John Wiley & Sons Ltd.

Encryption protection for communication satellites

Science.gov (United States)

Sood, D. R.; Hoernig, O. W., Jr.

In connection with the growing importance of the commercial communication satellite systems and the introduction of new technological developments, users and operators of these systems become increasingly concerned with aspects of security. The user community is concerned with maintaining confidentiality and integrity of the information being transmitted over the satellite links, while the satellite operators are concerned about the safety of their assets in space. In response to these concerns, the commercial satellite operators are now taking steps to protect the communication information and the satellites. Thus, communication information is being protected by end-to-end encryption of the customer communication traffic. Attention is given to the selection of the NBS DES algorithm, the command protection systems, and the communication protection systems.

Satellite Communications Industry

Science.gov (United States)

1993-04-01

Ariane $loom SAJAC 1 Hughes Satellite Japan 06/94 $150m SAJAC 2 Hughes Satellite Japan -- (spare) $150m SatcomHl GE GE Americom /95 $50m SOLIDARIDAD ...1 Hughes SCT (Mexico) 11/93 Ariane $loom SOLIDARIDAD 2 Hughes SCT (Mexico) /94 $loom Superbird Al Loral Space Com Gp (Jap) 11/92 Ariane $175m

23 CFR 500.108 - SMS.

Science.gov (United States)

2010-04-01

... SYSTEMS Management Systems § 500.108 SMS. An SMS is a systematic process with the goal of reducing the... Management Systems: Good Practices for Development and Implementation.” 3 An effective SMS should include, at a minimum: 3 Safety Management Systems: Good Practices for Development and Implementation, FHWA and...

Solar Power Satellites

CERN Document Server

Flournoy, Don M

2012-01-01

Communication satellites are a $144 billion industry. Is there any space-based industry that could possibly beat that market? 'Solar Power Satellites' shows why and how the space satellite industry will soon begin expanding its market from relaying signals to Earth to generating energy in space and delivering it to the ground as electricity. In all industrialized nations, energy demand is growing exponentially. In the developing world, the need for energy is as basic as food and water. The Sun's energy is available everywhere, and it is non-polluting. As business plans demonstrate its technical feasibility, commercial potential, and environmental acceptability, every country on Earth will look to space for the power it needs.

Geostationary satellites collocation

CERN Document Server

Li, Hengnian

2014-01-01

Geostationary Satellites Collocation aims to find solutions for deploying a safe and reliable collocation control. Focusing on the orbital perturbation analysis, the mathematical foundations for orbit and control of the geostationary satellite are summarized. The mathematical and physical principle of orbital maneuver and collocation strategies for multi geostationary satellites sharing with the same dead band is also stressed. Moreover, the book presents some applications using the above algorithms and mathematical models to help readers master the corrective method for planning station keeping maneuvers. Engineers and scientists in the fields of aerospace technology and space science can benefit from this book. Hengnian Li is the Deputy Director of State Key Laboratory of Astronautic Dynamics, China.

Optimized Fast-FISH with a-satellite probes: acceleration by microwave activation

Directory of Open Access Journals (Sweden)

Durm M.

1997-01-01

Full Text Available It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation time and the number of subsequent washing steps were reduced considerably by omitting denaturing chemical agents (e.g., formamide. The appropriate hybridization temperature and time allow a clear discrimination between major and minor binding sites by quantitative fluorescence microscopy. The well-defined physical conditions for hybridization permit automatization of the procedure, e.g., by a programmable thermal cycler. Here, we present optimized conditions for a commercially available X-specific a-satellite probe. Highly fluorescent major binding sites were obtained for 74oC hybridization temperature and 60 min hybridization time. They were clearly discriminated from some low fluorescent minor binding sites on metaphase chromosomes as well as in interphase cell nuclei. On average, a total of 3.43 ± 1.59 binding sites were measured in metaphase spreads, and 2.69 ± 1.00 in interphase nuclei. Microwave activation for denaturation and hybridization was tested to accelerate the procedure. The slides with the target material and the hybridization buffer were placed in a standard microwave oven. After denaturation for 20 s at 900 W, hybridization was performed for 4 min at 90 W. The suitability of a microwave oven for Fast-FISH was confirmed by the application to a chromosome 1-specific a-satellite probe. In this case, denaturation was performed at 630 W for 60 s and hybridization at 90 W for 5 min. In all cases, the results were analyzed quantitatively and compared to the results obtained by Fast-FISH. The major binding sites were clearly discriminated by their brightness

Muscle satellite cell heterogeneity and self-renewal

Science.gov (United States)

Motohashi, Norio; Asakura, Atsushi

2014-01-01

Adult skeletal muscle possesses extraordinary regeneration capacities. After muscle injury or exercise, large numbers of newly formed muscle fibers are generated within a week as a result of expansion and differentiation of a self-renewing pool of muscle stem cells termed muscle satellite cells. Normally, satellite cells are mitotically quiescent and reside beneath the basal lamina of muscle fibers. Upon regeneration, satellite cells are activated, and give rise to daughter myogenic precursor cells. After several rounds of proliferation, these myogenic precursor cells contribute to the formation of new muscle fibers. During cell division, a minor population of myogenic precursor cells returns to quiescent satellite cells as a self-renewal process. Currently, accumulating evidence has revealed the essential roles of satellite cells in muscle regeneration and the regulatory mechanisms, while it still remains to be elucidated how satellite cell self-renewal is molecularly regulated and how satellite cells are important in aging and diseased muscle. The number of satellite cells is decreased due to the changing niche during ageing, resulting in attenuation of muscle regeneration capacity. Additionally, in Duchenne muscular dystrophy (DMD) patients, the loss of satellite cell regenerative capacity and decreased satellite cell number due to continuous needs for satellite cells lead to progressive muscle weakness with chronic degeneration. Thus, it is necessary to replenish muscle satellite cells continuously. This review outlines recent findings regarding satellite cell heterogeneity, asymmetric division and molecular mechanisms in satellite cell self-renewal which is crucial for maintenance of satellite cells as a muscle stem cell pool throughout life. In addition, we discuss roles in the stem cell niche for satellite cell maintenance, as well as related cell therapies for approaching treatment of DMD. PMID:25364710

Muscle Satellite Cell Heterogeneity and Self-Renewal

Directory of Open Access Journals (Sweden)

Norio eMotohashi

2014-01-01

Full Text Available Adult skeletal muscle possesses extraordinary regeneration capacities. After muscle injury or exercise, large numbers of newly formed muscle fibers are generated within a week as a result of expansion and differentiation of a self-renewing pool of muscle stem cells termed muscle satellite cells. Normally, satellite cells are mitotically quiescent and reside beneath the basal lamina of muscle fibers. Upon regeneration, satellite cells are activated, and give rise to daughter myogenic precursor cells. After several rounds of proliferation, these myogenic precursor cells contribute to the formation of new muscle fibers. During cell division, a minor population of myogenic precursor cells returns to quiescent satellite cells as a self-renewal process. Currently, accumulating evidence has revealed the essential roles of satellite cells in muscle regeneration and the regulatory mechanisms, while it still remains to be elucidated how satellite cell self-renewal is molecularly regulated and how satellite cells are important in aging and diseased muscle. The number of satellite cells is decreased due to the changing niche during ageing, resulting in attenuation of muscle regeneration capacity. Additionally, in Duchenne muscular dystrophy (DMD patients, the loss of satellite cell regenerative capacity and decreased satellite cell number due to continuous needs for satellite cells lead to progressive muscle weakness with chronic degeneration. Thus, it is necessary to replenish muscle satellite cells continuously. This review outlines recent findings regarding satellite cell heterogeneity, asymmetric division and molecular mechanisms in satellite cell self-renewal which is crucial for maintenance of satellite cells as a muscle stem cell pool throughout life. In addition, we discuss roles in the stem cell niche for satellite cell maintenance, as well as related cell therapies for approaching treatment of DMD.

13 CFR 101.500 - Small Business Energy Efficiency Program.

Science.gov (United States)

2010-01-01

... making available online, the information and materials developed under the program to small business... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Small Business Energy Efficiency Program. 101.500 Section 101.500 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION...

31 CFR 500.404 - Transactions between principal and agent.

Science.gov (United States)

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Transactions between principal and agent. 500.404 Section 500.404 Money and Finance: Treasury Regulations Relating to Money and Finance... transaction were in no way affiliated or associated with each other. ...

Recursive analytical solution describing artificial satellite motion perturbed by an arbitrary number of zonal terms

Science.gov (United States)

Mueller, A. C.

1977-01-01

An analytical first order solution has been developed which describes the motion of an artificial satellite perturbed by an arbitrary number of zonal harmonics of the geopotential. A set of recursive relations for the solution, which was deduced from recursive relations of the geopotential, was derived. The method of solution is based on Von-Zeipel's technique applied to a canonical set of two-body elements in the extended phase space which incorporates the true anomaly as a canonical element. The elements are of Poincare type, that is, they are regular for vanishing eccentricities and inclinations. Numerical results show that this solution is accurate to within a few meters after 500 revolutions.

Multilayer x-ray mirrors for the objective crystal spectrometer on the Spectrum Roentgen Gamma satellite

DEFF Research Database (Denmark)

Louis, E.; Spiller, E.; Abdali, S.

1995-01-01

with Kr+- and Ar+- ions of 300, 500, and 1000 eV. We examined the effect of different polishing parameters on the smoothening of the Co- and Ni-layers. The in-situ reflectivity of lambda equals 3.16 nm during deposition and the ex-situ grazing incidence reflectivity of Cu-K(alpha ) radiation (lambda...... multiplied by 6 cm2 Si (111) crystals for the Objective Crystal Spectrometer on the Russian Spectrum Rontgen Gamma satellite. The coatings on the flight crystals have a period Lambda of 3.95 plus or minus 0.02 nm and a reflectivity of more than 8% averaged over s- and p-polarization over the entire...

Satellite communications network design and analysis

CERN Document Server

Jo, Kenneth Y

2011-01-01

This authoritative book provides a thorough understanding of the fundamental concepts of satellite communications (SATCOM) network design and performance assessments. You find discussions on a wide class of SATCOM networks using satellites as core components, as well as coverage key applications in the field. This in-depth resource presents a broad range of critical topics, from geosynchronous Earth orbiting (GEO) satellites and direct broadcast satellite systems, to low Earth orbiting (LEO) satellites, radio standards and protocols.This invaluable reference explains the many specific uses of

Satellites You Can See for Homework

Science.gov (United States)

Broderick, Stephen

2012-01-01

Artificial satellites are easily observed most nights when the weather is fine. The website called "Heavens Above" at www.heavens-above.com will help locate these satellites flying over one's location. It also includes how bright they will appear. The direction of travel of each satellite in the night sky also indicates the type of satellite. For…

Tri-allelic SNP markers enable analysis of mixed and degraded DNA samples.

Science.gov (United States)

Westen, Antoinette A; Matai, Anuska S; Laros, Jeroen F J; Meiland, Hugo C; Jasper, Mandy; de Leeuw, Wiljo J F; de Knijff, Peter; Sijen, Titia

2009-09-01

For the analysis of degraded DNA in disaster victim identification (DVI) and criminal investigations, single nucleotide polymorphisms (SNPs) have been recognized as promising markers mainly because they can be analyzed in short sized amplicons. Most SNPs are bi-allelic and are thereby ineffective to detect mixtures, which may lead to incorrect genotyping. We developed an algorithm to find non-binary (i.e. tri-allelic or tetra-allelic) SNPs in the NCBI dbSNP database. We selected 31 potential tri-allelic SNPs with a minor allele frequency of at least 10%. The tri-allelic nature was confirmed for 15 SNPs residing on 14 different chromosomes. Multiplex SNaPshot assays were developed, and the allele frequencies of 16 SNPs were determined among 153 Dutch and 111 Netherlands Antilles reference samples. Using these multiplex SNP assays, the presence of a mixture of two DNA samples in a ratio up to 1:8 could be recognized reliably. Furthermore, we compared the genotyping efficiency of the tri-allelic SNP markers and short tandem repeat (STR) markers by analyzing artificially degraded DNA and DNA from 30 approximately 500-year-old bone and molar samples. In both types of degraded DNA samples, the larger sized STR amplicons failed to amplify whereas the tri-allelic SNP markers still provided valuable information. In conclusion, tri-allelic SNP markers are suited for the analysis of degraded DNA and enable the detection of a second DNA source in a sample.

University Satellite Consortium and Space Education in Japan Centered on Micro-Nano Satellites

Science.gov (United States)

Nakasuka, S.; Kawashima, R.

2002-01-01

in Japan especially centered on micro or nano class satellites. Hands-on training using micro-nano satellites provide unique opportunity of space education to university level students, by giving them a chance to experience the whole space project cycle from mission creation, satellite design, fabrication, test, launch, operation through analysis of the results. Project management and team working are other important skills that can be trained in these projects. include 1) low cost, which allows one laboratory in university to carry out a project, 2) short development period such as one or two year, which enables students to obtain the results of their projects before they graduate, and 3) small size and weight, which enables fabrication and test within usually very narrow university laboratory areas. In Japan, several projects such as CanSat, CubeSat or Whale Observation Satellite have been carried out, proving that micro-nano satellites provide very unique and valuable educational opportunity. with the objective to make a university student and staff community of these micro-nano satellite related activities in Japan. This consortium aims for many activities including facilitating information and skills exchange and collaborations between member universities, helping students to use ground test facilities of national laboratories, consulting them on political or law related matters, coordinating joint development of equipments or projects, and bridging between these university activities and the needs or interests of the people in general. This kind of outreach activity is essential because how to create missions of micro-nano satellites should be pursued in order for this field to grow larger than a merely educational enterprise. The final objectives of the consortium is to make a huge community of the users, mission creators, investors and manufactures(i.e., university students) of micro-nano satellites, and provide a unique contribution to the activation of

Handbook of satellite applications

CERN Document Server

Madry, Scott; Camacho-Lara, Sergio

2013-01-01

Top space experts from around the world have collaborated to produce this comprehensive, authoritative, and clearly illustrated reference guide to the fast growing, multi-billion dollar field of satellite applications and space communications. This handbook, done under the auspices of the International Space University based in France, addresses not only system technologies but also examines market dynamics, technical standards and regulatory constraints. The handbook is a completely multi-disciplinary reference book that covers, in an in-depth fashion, the fields of satellite telecommunications, Earth observation, remote sensing, satellite navigation, geographical information systems, and geosynchronous meteorological systems. It covers current practices and designs as well as advanced concepts and future systems. It provides a comparative analysis of the common technologies and design elements for satellite application bus structures, thermal controls, power systems, stabilization techniques, telemetry, com...

Sky alert! when satellites fail

CERN Document Server

Johnson, Les

2013-01-01

How much do we depend on space satellites? Defense, travel, agriculture, weather forecasting, mobile phones and broadband, commerce...the list seems endless. But what would our live be like if the unimaginable happened and, by accident or design, those space assets disappeared? Sky Alert! explores what our world would be like, looking in turn at areas where the loss could have catastrophic effects. The book - demonstrates our dependence on space technology and satellites; - outlines the effect on our economy, defense, and daily lives if satellites and orbiting spacecraft were destroyed; - illustrates the danger of dead satellites, spent rocket stages, and space debris colliding with a functioning satellites; - demonstrates the threat of dramatically increased radiation levels associated with geomagnetic storms; - introduces space as a potential area of conflict between nations.

Dose-dependent reduction of 3,2'-dimethyl-4-aminobiphenyl-derived DNA adducts in colon and liver of rats administered celecoxib

International Nuclear Information System (INIS)

Ravoori, Srivani; Feng Yi; Neale, Jason R.; Jeyabalan, Jeyaprakash; Srinivasan, Cidambi; Hein, David W.; Gupta, Ramesh C.

2008-01-01

Colon cancer is second leading cause of cancer-related deaths in Western countries. Diet and smoking, which contain aromatic and heterocyclic amines, are major risk factors for colon cancer. Colorectal cancers have a natural history of long latency and therefore provide ample opportunities for effective chemoprevention. 3,2'-Dimethyl-4-aminobiphenyl (DMABP) is an experimental aromatic amine that causes cancer in rat colon and serves as an experimental model for arylamine and heterocyclic amine mutagens derived from diet and smoking. In this study, we investigated the effects of celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor on DMABP-induced DNA adduct formation in rat liver and colon. Male F-344 rats (5-week old) were provided free access to modified AIN-76A rat chow containing 0 (control), 500, 1000, or 1500 ppm celecoxib. Two weeks later, the rats received a subcutaneous injection of 100 mg/kg DMABP in peanut oil. Two days after DMABP treatment, the rats were killed and DMABP-derived adducts were analyzed in colon and liver DNA by butanol extraction-mediated 32 P-postlabeling. Two major DNA adducts, identified as dG-C8-DMABP and dG-N 2 -DMABP, were detected in liver and colon of rats treated with DMABP. These DNA adducts were diminished approximately 35-40% with 500 ppm and 65-70% with 1,000 ppm celecoxib. In the colon, no further decline in DNA adducts was observed at 1500 ppm. The same DMABP-DNA adducts also were detected in the liver and were also diminished by celecoxib treatment. The reduction in DMABP-DNA adduct levels in celecoxib-treated animals provides further support for celecoxib as a chemopreventive agent for colorectal cancer

Construction of a T7 Human Lung Cancer cDNA Library

Directory of Open Access Journals (Sweden)

Wentao YUE

2008-10-01

Full Text Available Background and objective Currently, only a limited numbers of tumor markers for non small lung cancer (NSCLC diagnosis, new biomarker, such as serum autoantibody may improve the early detection of lung cancer. Our objective is construction human lung squamous carcinoma and adenocarcinoma T7 phage display cDNA library from the tissues of NSCLC patients. Methods mRNA was isolated from a pool of total RNA extract from NSCLC tissues obtained from 5 adenocarcinomas and 5 squamous carcinomas, and then mRNA was reverse transcribed into double stranded cDNA. After digestion, the cDNA was inserted into T7Select 10-3 vector. The phage display cDNA library was constructed by package reaction in vitro and plate proliferation. Plaque assay and PCR were used to evaluate the library.Results Two T7 phage display cDNA library were established. Plaque assay show the titer of lung squamas carcinoma library was 1.8×106 pfu, and the adenocarcinoma library was 5×106 pfu. The phage titer of the amplified library were 3.2×1010 pfu/mL and 2.5×1010 pfu/mL. PCR amplification of random plaque show insert ratio were 100% (24/24 in adenocarcinoma library and 95.8% in human lung squamas carcinoma library (23/24. Insert range from 300 bp to 1 500 bp. Conclusion Two phage display cDNA library from NSCLC were constructed.

Spectroscopic (UV/VIS, Raman and Electrophoresis Study of Cytosine-Guanine Oligonucleotide DNA Influenced by Magnetic Field.

Directory of Open Access Journals (Sweden)

Seyedeh Maryam Banihashemian

Full Text Available Studying the effect of a magnetic field on oligonucleotide DNA can provide a novel DNA manipulation technique for potential application in bioengineering and medicine. In this work, the optical and electrochemical response of a 100 bases oligonucleotides DNA, cytosine-guanine (CG100, is investigated via exposure to different magnetic fields (250, 500, 750, and 1000 mT. As a result of the optical response of CG100 to the magnetic field, the ultra-violet-visible spectrum indicated a slight variation in the band gap of CG100 of about 0.3 eV. Raman spectroscopy showed a significant deviation in hydrogen and phosphate bonds' vibration after exposure to the magnetic field. Oligonucleotide DNA mobility was investigated in the external electric field using the gel electrophoresis technique, which revealed a small decrease in the migration of CG100 after exposure to the magnetic field.