NODC Standard Product: NODC Taxonomic Code on CD-ROM (NODC Accession 0050418)
National Oceanic and Atmospheric Administration, Department of Commerce — The content of the NODC Taxonomic Code, Version 8 CD-ROM (CD-ROM NODC-68) distributed by NODC is archived in this accession. Version 7 of the NODC Taxonomic Code...
Directory of Open Access Journals (Sweden)
Simone Bortolan
2009-11-01
Full Text Available O objetivo deste trabalho foi avaliar a expressão, por RT-qPCR, dos genes de nodulação nodC e nodW e do gene nopP da estirpe CPAC 15, que provavelmente atuam na infecção das raízes da soja. Foram realizados dois experimentos. No primeiro, a expressão dos genes foi avaliada nas células após a incubação com genisteína por 15 min, 1, 4 e 8 horas. Os resultados revelaram que os três genes apresentaram maior expressão imediatamente após o contato com o indutor (15 min. No segundo experimento, a bactéria foi cultivada na presença de indutores (genisteína ou exsudatos de sementes de soja por 48 horas. A expressão dos três genes foi maior na presença de genisteína, com valores de expressão para nodC, nodW e nopP superiores ao controle. Os resultados obtidos confirmam a funcionalidade dos três genes na estirpe CPAC 15, com ênfase para o nopP, cuja funcionalidade em Bradyrhizobium japonicum foi descrita pela primeira vez.The objective of this work was to evaluate, by RT-qPCR, the expression of the nodC and nodW nodulation genes and of the nopP gene of the CPAC 15 strain, which probably play a role in the infection of soybean roots. Two experiments were done. In the first, the gene expression was evaluated in cells after incubation with genistein for 15 min, 1, 4 and 8 hours. Results showed that the three genes showed higher expression immediately after contact with the inducer (15 min. In the second experiment, the bacterium was grown in the presence of inducers (genistein or soybean seed exudates for 48 hours. The expression of the three genes was greater when induced by genistein, and the expression of nodC, nodW and nopP had higher values than the control. The results confirm the functionality of the three genes in the CPAC 15 strain, with an emphasis on the nopP, whose functionality in Bradyrhizobium japonicum was described for the first time.
Directory of Open Access Journals (Sweden)
Heng Wee Tan
Full Text Available New Zealand became geographically isolated about 80 million years ago and this separation gave rise to a unique native flora including four genera of legume, Carmichaelia, Clianthus and Montigena in the Carmichaelinae clade, tribe Galegeae, and Sophora, tribe Sophoreae, sub-family Papilionoideae. Ten bacterial strains isolated from NZ Carmichaelinae growing in natural ecosystems grouped close to the Mesorhizobium huakuii type strain in relation to their 16S rRNA and nifH gene sequences. However, the ten strains separated into four groups on the basis of their recA and glnII sequences: all groups were clearly distinct from all Mesorhizobium type strains. The ten strains separated into two groups on the basis of their nodA sequences but grouped closely together in relation to nodC sequences; all nodA and nodC sequences were novel. Seven strains selected and the M. huakuii type strain (isolated from Astragalus sinicus produced functional nodules on Carmichaelia spp., Clianthus puniceus and A. sinicus but did not nodulate two Sophora species. We conclude that rhizobia closely related to M. huakuii on the basis of 16S rRNA and nifH gene sequences, but with variable recA and glnII genes and novel nodA and nodC genes, are common symbionts of NZ Carmichaelinae.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC maintains data in three NODC Standard Format Marine Mammal Data Sets: Marine Mammal Sighting and Census (F127); Marine Mammal Specimens (F025); Marine Mammal...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC maintains data in three NODC Standard Format Marine Mammal Data Sets: Marine Mammal Sighting and Census (F127); Marine Mammal Specimens (F025); Marine Mammal...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC maintains data in three NODC Standard Format Marine Mammal Data Sets: Marine Mammal Sighting and Census (F127); Marine Mammal Specimens (F025); Marine Mammal...
National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanographic Data Center (NODC) created a compact disk containing over 1.3 million temperature-depth and salinity-depth profiles taken in the Pacific...
NODC Standard Product: Oceanographic station profile time series (NODC Accession 0095191)
National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanographic Data Center and the World Data Center-A for Oceanography compiled from the NODC Oceanographic Station Data File a set of oceanographic...
Bioinformatics approach of three partial polyprenol reductase genes in Kandelia obovata
Basyuni, M.; Wati, R.; Sagami, H.; Oku, H.; Baba, S.
2018-03-01
This present study describesthe bioinformatics approach to analyze three partial polyprenol reductase genes from mangrove plant, Kandeliaobovataas well aspredictedphysical and chemical properties, potential peptide, subcellular localization, and phylogenetic. The diversity was noted in the physical and chemical properties of three partial polyprenol reductase genes. The values of chloroplast were relatively high, showed that chloroplast transit peptide occurred in mangrove polyprenol reductase. The target peptide value of mitochondria varied from 0.088 to 0.198 indicated it was possible to be present. These results suggested the importance of understanding the diversity of physicochemical properties of the different amino acids in polyprenol reductase. The subcellular localization of two partial genes located in the plasma membrane. To confirm the homology among the polyprenol reductase in the database, a dendrogram was drawn. The phylogenetic tree depicts that there are three clusters, the partial genes of K. obovata joined the largest one: C23157 was close to Ricinus communis polyprenol reductase. Whereas, C23901 and C24171 were grouped with Ipomoea nil polyprenol reductase, suggested that these polyprenol reductase genes form distinct separation into tropical habitat plants.
and Epigenetic Dysregulation in Diabetes-prone Bicongenic B6.NODC11bxC1tb Mice
Directory of Open Access Journals (Sweden)
Erin Garrigan
2015-01-01
Full Text Available In Type 1 diabetic (T1D human monocytes, STAT5 aberrantly binds to epigenetic regulatory sites of two proinflammatory genes, CSF2 (encoding granulocyte–macrophage colony-stimulating factor and PTGS2 (encoding prostaglandin synthase 2/cyclooxygenase 2. Bicongenic B6.NOD C11bxC1tb mice re-create this phenotype of T1D monocytes with only two nonobese diabetic (NOD Idd subloci (130.8 Mb–149.7 Mb, of Idd5 on Chr 1 and 32.08–53.85 Mb of Idd4.3 on Chr11 on C57BL/6 genetic background. These two Idd loci interact through STAT5 binding at upstream regulatory regions affecting Csf2 ( Chr 11 and Ptgs2 ( Chr 1 expression. B6.NODC11bxC1tb mice exhibited hyperglycemia and immune destruction of pancreatic islets between 8 and 30 weeks of age, with 12%–22% penetrance. Thus, B6.NODC11bxC1tb mice embody NOD epigenetic dysregulation of gene expression in myeloid cells, and this defect appears to be sufficient to impart genetic susceptibility to diabetes in an otherwise genetically nonautoimmune mouse.
National Oceanic and Atmospheric Administration, Department of Commerce — This set of CD-ROMs contains global ocean temperature and salinity profiles derived from NODC archive data files. It includes oceanographic station (bottle) data,...
Casey, K. S.; Li, Y.
2011-12-01
The US National Oceanographic Data Center (NODC) has implemented numerous interoperable data technologies in recent years to enhance the discovery, understanding, and use of the vast quantities of data in the NODC archives. These services include OPeNDAP's Hyrax server, Unidata's THREDDS Data Server (TDS), NOAA's Live Access Server (LAS), and most recently the ESRI ArcGIS Server. Combined, these technologies enable NODC to provide access to its data holdings and products through most of the commonly-used standardized web services like the Data Access Protocol (DAP) and the Open Geospatial Consortium suite of services such as the Web Mapping Service (WMS) and Web Coverage Service (WCS). Despite the strong demand for and use of these services, the acronym-rich environment of services can also result in confusion for producers of data to the NODC archives, for consumers of data from the NODC archives, and for the data stewards at the archives as well. The situation is further complicated by the fact that NODC also maintains some ad hoc services like WODselect, and that not all services can be applied to all of the tens of thousands of collections in the NODC archive; where once every data set was available only through FTP and HTTP servers, now many are also available from the LAS, TDS, Hyrax, and ArcGIS Server. To bring order and clarity to this potentially confusing collection of services, NODC deployed the Geoportal Server into its Archive Management System as an integrating technology that brings together its various data access, visualization, and discovery services as well as its overall metadata management workflows. While providing an enhanced web-based interface for more integrated human-to-machine discovery and access, the deployment also enables NODC for the first time to support a robust set of machine-to-machine discovery services such as the Catalog Service for the Web (CS/W), OpenSearch, and Search and Retrieval via URL (SRU) . This approach allows NODC
National Oceanic and Atmospheric Administration, Department of Commerce — This archival information package contains a listing of codes and chemical names that were used in NODC Standard Format Marine Toxic Substances and Pollutants (F144)...
2000-01-01
The Kenya National Oceanographic Data Center (KeNODC) began operating in January 1997. The first set of activities included identification of staff members and setting up of office infrastructure. Amidst all this, the Center conducted the first planning mission in March 1997 to set out priorities for action. Foremost were the duty allocations among the four staff members designated by KMFRI. This has been followed by a familiarization of a number of IODE policy documents and manuals for op...
Directory of Open Access Journals (Sweden)
Sarah K Meadows
2010-07-01
Full Text Available In the event of a terrorist-mediated attack in the United States using radiological or improvised nuclear weapons, it is expected that hundreds of thousands of people could be exposed to life-threatening levels of ionizing radiation. We have recently shown that genome-wide expression analysis of the peripheral blood (PB can generate gene expression profiles that can predict radiation exposure and distinguish the dose level of exposure following total body irradiation (TBI. However, in the event a radiation-mass casualty scenario, many victims will have heterogeneous exposure due to partial shielding and it is unknown whether PB gene expression profiles would be useful in predicting the status of partially irradiated individuals. Here, we identified gene expression profiles in the PB that were characteristic of anterior hemibody-, posterior hemibody- and single limb-irradiation at 0.5 Gy, 2 Gy and 10 Gy in C57Bl6 mice. These PB signatures predicted the radiation status of partially irradiated mice with a high level of accuracy (range 79-100% compared to non-irradiated mice. Interestingly, PB signatures of partial body irradiation were poorly predictive of radiation status by site of injury (range 16-43%, suggesting that the PB molecular response to partial body irradiation was anatomic site specific. Importantly, PB gene signatures generated from TBI-treated mice failed completely to predict the radiation status of partially irradiated animals or non-irradiated controls. These data demonstrate that partial body irradiation, even to a single limb, generates a characteristic PB signature of radiation injury and thus may necessitate the use of multiple signatures, both partial body and total body, to accurately assess the status of an individual exposed to radiation.
WATER DEPTH and Other Data (NODC Accession 9200271)
National Oceanic and Atmospheric Administration, Department of Commerce — The data in this accession was submitted to NODC as part of Data Rescue & Archaeology project by International Council for Exploration of the Sea (ICES),...
DEPTH - OBSERVATION and Other Data (NODC Accession 9600038)
National Oceanic and Atmospheric Administration, Department of Commerce — The Temperature, Salinity and Chemistry data from 12 stations in this accession was digitized at NODC from hardcopy. The data was collected as part of Global Ocean...
PPARγ partial agonist GQ-16 strongly represses a subset of genes in 3T3-L1 adipocytes
Energy Technology Data Exchange (ETDEWEB)
Milton, Flora Aparecida [Faculdade de Ciências da Saúde, Laboratório de Farmacologia Molecular, Universidade de Brasília (Brazil); Genomic Medicine, Houston Methodist Research Institute, Houston, TX (United States); Cvoro, Aleksandra [Genomic Medicine, Houston Methodist Research Institute, Houston, TX (United States); Amato, Angelica A. [Faculdade de Ciências da Saúde, Laboratório de Farmacologia Molecular, Universidade de Brasília (Brazil); Sieglaff, Douglas H.; Filgueira, Carly S.; Arumanayagam, Anithachristy Sigamani [Genomic Medicine, Houston Methodist Research Institute, Houston, TX (United States); Caro Alves de Lima, Maria do; Rocha Pitta, Ivan [Laboratório de Planejamento e Síntese de Fármacos – LPSF, Universidade Federal de Pernambuco (Brazil); Assis Rocha Neves, Francisco de [Faculdade de Ciências da Saúde, Laboratório de Farmacologia Molecular, Universidade de Brasília (Brazil); Webb, Paul, E-mail: pwebb@HoustonMethodist.org [Genomic Medicine, Houston Methodist Research Institute, Houston, TX (United States)
2015-08-28
Thiazolidinediones (TZDs) are peroxisome proliferator-activated receptor gamma (PPARγ) agonists that improve insulin resistance but trigger side effects such as weight gain, edema, congestive heart failure and bone loss. GQ-16 is a PPARγ partial agonist that improves glucose tolerance and insulin sensitivity in mouse models of obesity and diabetes without inducing weight gain or edema. It is not clear whether GQ-16 acts as a partial agonist at all PPARγ target genes, or whether it displays gene-selective actions. To determine how GQ-16 influences PPARγ activity on a gene by gene basis, we compared effects of rosiglitazone (Rosi) and GQ-16 in mature 3T3-L1 adipocytes using microarray and qRT-PCR. Rosi changed expression of 1156 genes in 3T3-L1, but GQ-16 only changed 89 genes. GQ-16 generally showed weak effects upon Rosi induced genes, consistent with partial agonist actions, but a subset of modestly Rosi induced and strongly repressed genes displayed disproportionately strong GQ-16 responses. PPARγ partial agonists MLR24 and SR1664 also exhibit disproportionately strong effects on transcriptional repression. We conclude that GQ-16 displays a continuum of weak partial agonist effects but efficiently represses some negatively regulated PPARγ responsive genes. Strong repressive effects could contribute to physiologic actions of GQ-16. - Highlights: • GQ-16 is an insulin sensitizing PPARγ ligand with reduced harmful side effects. • GQ-16 displays a continuum of weak partial agonist activities at PPARγ-induced genes. • GQ-16 exerts strong repressive effects at a subset of genes. • These inhibitor actions should be evaluated in models of adipose tissue inflammation.
PPARγ partial agonist GQ-16 strongly represses a subset of genes in 3T3-L1 adipocytes
International Nuclear Information System (INIS)
Milton, Flora Aparecida; Cvoro, Aleksandra; Amato, Angelica A.; Sieglaff, Douglas H.; Filgueira, Carly S.; Arumanayagam, Anithachristy Sigamani; Caro Alves de Lima, Maria do; Rocha Pitta, Ivan; Assis Rocha Neves, Francisco de; Webb, Paul
2015-01-01
Thiazolidinediones (TZDs) are peroxisome proliferator-activated receptor gamma (PPARγ) agonists that improve insulin resistance but trigger side effects such as weight gain, edema, congestive heart failure and bone loss. GQ-16 is a PPARγ partial agonist that improves glucose tolerance and insulin sensitivity in mouse models of obesity and diabetes without inducing weight gain or edema. It is not clear whether GQ-16 acts as a partial agonist at all PPARγ target genes, or whether it displays gene-selective actions. To determine how GQ-16 influences PPARγ activity on a gene by gene basis, we compared effects of rosiglitazone (Rosi) and GQ-16 in mature 3T3-L1 adipocytes using microarray and qRT-PCR. Rosi changed expression of 1156 genes in 3T3-L1, but GQ-16 only changed 89 genes. GQ-16 generally showed weak effects upon Rosi induced genes, consistent with partial agonist actions, but a subset of modestly Rosi induced and strongly repressed genes displayed disproportionately strong GQ-16 responses. PPARγ partial agonists MLR24 and SR1664 also exhibit disproportionately strong effects on transcriptional repression. We conclude that GQ-16 displays a continuum of weak partial agonist effects but efficiently represses some negatively regulated PPARγ responsive genes. Strong repressive effects could contribute to physiologic actions of GQ-16. - Highlights: • GQ-16 is an insulin sensitizing PPARγ ligand with reduced harmful side effects. • GQ-16 displays a continuum of weak partial agonist activities at PPARγ-induced genes. • GQ-16 exerts strong repressive effects at a subset of genes. • These inhibitor actions should be evaluated in models of adipose tissue inflammation
Characterization of five partial deletions of the factor VIII gene
International Nuclear Information System (INIS)
Youssoufian, H.; Antonarakis, S.E.; Aronis, S.; Tsiftis, G.; Phillips, D.G.; Kazazian, H.H. Jr.
1987-01-01
Hemophilia A is an X-linked disorder of coagulation caused by a deficiency of factor VIII. By using cloned DNA probes, the authors have characterized the following five different partial deletions of the factor VIII gene from a panel of 83 patients with hemophilia A: (i) a 7-kilobase (kb) deletion that eliminates exon 6; (ii) a 2.5-kb deletion that eliminates 5' sequences of exon 14; (iii) a deletion of at least 7 kb that eliminates exons 24 and 25; (iv) a deletion of at least 16 kb that eliminates exons 23-25; and (v) a 5.5-kb deletion that eliminates exon 22. The first four deletions are associated with severe hemophilia A. By contrast, the last deletion is associated with moderate disease, possibly because of in-frame splicing from adjacent exons. None of those patients with partial gene deletions had circulating inhibitors to factor VIII. One deletion occurred de novo in a germ cell of the maternal grandmother, while a second deletion occurred in a germ cell of the maternal grandfather. These observations demonstrate that de novo deletions of X-linked genes can occur in either male or female gametes
National Oceanic and Atmospheric Administration, Department of Commerce — NODC produced twelve (12) CD-ROMs containing WOCE Version 1 project data in the summer of 1998. NODC produced fifteen (15) CD-ROMs containing WOCE Version 2 project...
Greenland-Iceland-Norwegian Seas Regional Climatology (NODC Accession 0112824)
National Oceanic and Atmospheric Administration, Department of Commerce — To provide an improved oceanographic foundation and reference for multi-disciplinary studies of the Greenland-Iceland-Norwegian Seas (GINS), NODC developed a new set...
National Oceanic and Atmospheric Administration, Department of Commerce — Marine Bird Surveys (F041) is one of a group of seven datasets related to Marine Birds from Coastal Alaska and Puget Sound Data (1979). Each dataset uses the NODC...
National Oceanic and Atmospheric Administration, Department of Commerce — Feeding Flock (F037) is one of a group of seven datasets related to Marine Birds from Coastal Alaska and Puget Sound Data (1974 -1982). Each dataset uses the NODC...
Partial characterization of three β-defensin gene transcripts in river ...
African Journals Online (AJOL)
In this study, the tracheal tissues from Egyptian river buffalo and cattle were screened for the presence of three bovine β-defensin gene transcripts. Three primer pairs were designed on the basis of published Bos taurus sequences for partial amplification of β-defensin 4, β-defensin 10 and β-defensin 11 complementary DNA ...
National Oceanic and Atmospheric Administration, Department of Commerce — This Atlas, Climatic Atlas of the Sea of Azov 2008 on CD-ROM, is an update to Volume 10, Climatic Atlas of the Sea of Azov 2006 on CD-ROM (NODC Accession 0098572),...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Standard Marine Bird Sighting, Land Census (F034) is one of a group of seven datasets related to Marine Birds from Coastal Alaska and Puget Sound Data (1975...
Persson, Tomas; Battenberg, Kai; Demina, Irina V.; Vigil-Stenman, Theoden; Vanden Heuvel, Brian; Pujic, Petar; Facciotti, Marc T.; Wilbanks, Elizabeth G.; O'Brien, Anna; Fournier, Pascale; Cruz Hernandez, Maria Antonia; Mendoza Herrera, Alberto; Médigue, Claudine; Normand, Philippe; Pawlowski, Katharina; Berry, Alison M.
2015-01-01
Frankia strains are nitrogen-fixing soil actinobacteria that can form root symbioses with actinorhizal plants. Phylogenetically, symbiotic frankiae can be divided into three clusters, and this division also corresponds to host specificity groups. The strains of cluster II which form symbioses with actinorhizal Rosales and Cucurbitales, thus displaying a broad host range, show suprisingly low genetic diversity and to date can not be cultured. The genome of the first representative of this cluster, Candidatus Frankia datiscae Dg1 (Dg1), a microsymbiont of Datisca glomerata, was recently sequenced. A phylogenetic analysis of 50 different housekeeping genes of Dg1 and three published Frankia genomes showed that cluster II is basal among the symbiotic Frankia clusters. Detailed analysis showed that nodules of D. glomerata, independent of the origin of the inoculum, contain several closely related cluster II Frankia operational taxonomic units. Actinorhizal plants and legumes both belong to the nitrogen-fixing plant clade, and bacterial signaling in both groups involves the common symbiotic pathway also used by arbuscular mycorrhizal fungi. However, so far, no molecules resembling rhizobial Nod factors could be isolated from Frankia cultures. Alone among Frankia genomes available to date, the genome of Dg1 contains the canonical nod genes nodA, nodB and nodC known from rhizobia, and these genes are arranged in two operons which are expressed in D. glomerata nodules. Furthermore, Frankia Dg1 nodC was able to partially complement a Rhizobium leguminosarum A34 nodC::Tn5 mutant. Phylogenetic analysis showed that Dg1 Nod proteins are positioned at the root of both α- and β-rhizobial NodABC proteins. NodA-like acyl transferases were found across the phylum Actinobacteria, but among Proteobacteria only in nodulators. Taken together, our evidence indicates an Actinobacterial origin of rhizobial Nod factors. PMID:26020781
National Oceanic and Atmospheric Administration, Department of Commerce — In 2002, the US Naval Oceanographic Office (NAVOCEANO) submitted these data to NODC in accordance with a Memorandum of Agreement between NODC and NAVOCEANO to...
Partial Gene Cloning and Enzyme Structure Modeling of Exolevanase Fragment from Bacillus subtilis
Azhar, M.; Natalia, D.; Syukur, S.; Andriani, N.; Jamsari, J.
2018-04-01
Inulin hydrolysis thermophilic and thermotolerant bacteria are potential sources of inulin hydrolysis enzymes. Partial gene that encodes inulin hydrolysis enzymes had been isolated from Bacillus subtilis using polymerase chain reaction (PCR) method with the DPE.slFandDPE.eR degenerative primers. The partial gene was cloned into pGEM-T Easy vector with E. coli as host cells and analyzed using BLASTx, CrustalW2, and Phyre2 programs. Size of thepartial gene had been found539 bp that encoded 179aminoacid residues of protein fragment. The sequences of protein fragment was more similar to exolevanase than exoinulinase. The protein fragment had conserved motif FSGS, and specific hits GH32 β-fructosidase. It had three residues of active site and five residues of substrate binding. The active site on the protein fragment were D (1-WLNDP-5), D (125-FRDPK-129) and E (177-WEC-179). Substrate binding on the protein fragment were ND (1-WLNDP-5), Q (18-FYQY-21), FS (60-FSGS-63) RD (125-FRDPK-129) and E (177-WEC-179).
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109930 includes biological, chemical, meteorological, physical and underway - surface data collected from NOAA Ship RONALD H. BROWN in the North...
Gnat, Sebastian; Małek, Wanda; Oleńska, Ewa; Wdowiak-Wróbel, Sylwia; Kalita, Michał; Łotocka, Barbara; Wójcik, Magdalena
2015-01-01
The phylogeny of symbiotic genes of Astragalus glycyphyllos L. (liquorice milkvetch) nodule isolates was studied by comparative sequence analysis of nodA, nodC, nodH and nifH loci. In all these genes phylograms, liquorice milkvetch rhizobia (closely related to bacteria of three species, i.e. Mesorhizobium amorphae, Mesorhizobium septentrionale and Mesorhizobium ciceri) formed one clearly separate cluster suggesting the horizontal transfer of symbiotic genes from a single ancestor to the bacteria being studied. The high sequence similarity of the symbiotic genes of A. glycyphyllos rhizobia (99-100% in the case of nodAC and nifH genes, and 98-99% in the case of nodH one) points to the relatively recent (in evolutionary scale) lateral transfer of these genes. In the nodACH and nifH phylograms, A. glycyphyllos nodule isolates were grouped together with the genus Mesorhizobium species in one monophyletic clade, close to M. ciceri, Mesorhizobium opportunistum and Mesorhizobium australicum symbiovar biserrulae bacteria, which correlates with the close relationship of these rhizobia host plants. Plant tests revealed the narrow host range of A. glycyphyllos rhizobia. They formed effective symbiotic interactions with their native host (A. glycyphyllos) and Amorpha fruticosa but not with 11 other fabacean species. The nodules induced on A. glycyphyllos roots were indeterminate with apical, persistent meristem, an age gradient of nodule tissues and cortical vascular bundles. To reflect the symbiosis-adaptive phenotype of rhizobia, specific for A. glycyphyllos, we propose for these bacteria the new symbiovar "glycyphyllae", based on nodA and nodC genes sequences.
Directory of Open Access Journals (Sweden)
Manju Soman
2018-04-01
Full Text Available Aim: This study aims at cloning, sequencing, and phylogenetic analysis of a partial CDS of ligA gene in pET-32a - Escherichia coli DH5α system, with the objective of identifying the conserved nature of the ligA gene in the genus Leptospira. Materials and Methods: A partial CDS (nucleotide 1873 to nucleotide 3363 of the ligA gene was amplified from genomic DNA of Leptospira interrogans serovar Canicola by polymerase chain reaction (PCR. The PCR-amplified DNA was cloned into pET-32a vector and transformed into competent E. coli DH5α bacterial cells. The partial ligA gene insert was sequenced and the nucleotide sequences obtained were aligned with the published ligA gene sequences of other Leptospira serovars, using nucleotide BLAST, NCBI. Phylogenetic analysis of the gene sequence was done by maximum likelihood method using Mega 6.06 software. Results: The PCR could amplify the 1491 nucleotide sequence spanning from nucleotide 1873 to nucleotide 3363 of the ligA gene and the partial ligA gene could be successfully cloned in E. coli DH5α cells. The nucleotide sequence when analyzed for homology with the reported gene sequences of other Leptospira serovars was found to have 100% homology to the 1910 bp to 3320 bp sequence of ligA gene of L. interrogans strain Kito serogroup Canicola. The predicted protein consisted of 470 aminoacids. Phylogenetic analysis revealed that the ligA gene was conserved in L. interrogans species. Conclusion: The partial ligA gene could be successfully cloned and sequenced from E. coli DH5α cells. The sequence showed 100% homology to the published ligA gene sequences. The phylogenetic analysis revealed the conserved nature of the ligA gene. Further studies on the expression and immunogenicity of the partial LigA protein need to be carried out to determine its competence as a subunit vaccine candidate.
Outbreak tracking of Aleutian mink disease virus (AMDV) using partial NS1 gene sequencing
DEFF Research Database (Denmark)
Ryt-Hansen, Pia; Hjulsager, Charlotte Kristiane; Hagberg, E. E.
2017-01-01
. However, in 2015, several outbreaks of AMDV occurred at mink farms throughout Denmark, and the sources of these outbreaks were not known. Partial NS1 gene sequencing, phylogenetic analyses data were utilized along with epidemiological to determine the origin of the outbreaks. The phylogenetic analyses...... not be excluded. This study confirmed that partial NS1 sequencing can be used in outbreak tracking to determine major viral clusters of AMDV. Using this method, two new distinct AMDV clusters with low intra-cluster sequence diversity were identified, and epidemiological data helped to reveal possible ways...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109917 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 2006-07-12 to 2006-08-06...
Fengler, Svenja; Spirer, Ina; Neef, Maren; Ecke, Margret; Hauslage, Jens; Hampp, Rüdiger
2016-06-01
Cell cultures of the plant model organism Arabidopsis thaliana were exposed to partial- g forces during parabolic flight and clinostat experiments (0.16 g, 0.38 g and 0.5 g were tested). In order to investigate gravity-dependent alterations in gene expression, samples were metabolically quenched by the fixative RNA later Ⓡ to stabilize nucleic acids and used for whole-genome microarray analysis. An attempt to identify the potential threshold acceleration for the gravity-dependent response showed that the smaller the experienced g-force, the greater was the susceptibility of the cell cultures. Compared to short-term μ g during a parabolic flight, the number of differentially expressed genes under partial- g was lower. In addition, the effect on the alteration of amounts of transcripts decreased during partial- g parabolic flight due to the sequence of the different parabolas (0.38 g, 0.16 g and μ g). A time-dependent analysis under simulated 0.5 g indicates that adaptation occurs within minutes. Differentially expressed genes (at least 2-fold up- or down-regulated in expression) under real flight conditions were to some extent identical with those affected by clinorotation. The highest number of homologuous genes was detected within seconds of exposure to 0.38 g (both flight and clinorotation). To a considerable part, these genes deal with cell wall properties. Additionally, responses specific for clinorotation were observed.
Directory of Open Access Journals (Sweden)
Yinyin Yuan
Full Text Available Inferring regulatory relationships among many genes based on their temporal variation in transcript abundance has been a popular research topic. Due to the nature of microarray experiments, classical tools for time series analysis lose power since the number of variables far exceeds the number of the samples. In this paper, we describe some of the existing multivariate inference techniques that are applicable to hundreds of variables and show the potential challenges for small-sample, large-scale data. We propose a directed partial correlation (DPC method as an efficient and effective solution to regulatory network inference using these data. Specifically for genomic data, the proposed method is designed to deal with large-scale datasets. It combines the efficiency of partial correlation for setting up network topology by testing conditional independence, and the concept of Granger causality to assess topology change with induced interruptions. The idea is that when a transcription factor is induced artificially within a gene network, the disruption of the network by the induction signifies a genes role in transcriptional regulation. The benchmarking results using GeneNetWeaver, the simulator for the DREAM challenges, provide strong evidence of the outstanding performance of the proposed DPC method. When applied to real biological data, the inferred starch metabolism network in Arabidopsis reveals many biologically meaningful network modules worthy of further investigation. These results collectively suggest DPC is a versatile tool for genomics research. The R package DPC is available for download (http://code.google.com/p/dpcnet/.
Physical and chemical properties of San Francisco Bay waters, 1969-1976 (NODC Accession 8400194)
National Oceanic and Atmospheric Administration, Department of Commerce — One magnetic tape containing the physical and chemical properties of San Francisco Bay waters was forwarded to NODC by Mr. Richard Smith of the U.S Geological Survey...
Directory of Open Access Journals (Sweden)
Guallar Jordi P
2008-08-01
Full Text Available Abstract Introduction Acquired partial lipodystrophy or Barraquer-Simons syndrome is a rare form of progressive lipodystrophy. The etiopathogenesis of adipose tissue atrophy in these patients is unknown. Case presentation This is a case report of a 44-year-old woman with acquired partial lipodystrophy. To obtain insight into the molecular basis of lipoatrophy in acquired partial lipodystrophy, we examined gene expression in adipose tissue from this patient newly diagnosed with acquired partial lipodystrophy. A biopsy of subcutaneous adipose tissue was obtained from the patient, and DNA and RNA were extracted in order to evaluate mitochondrial DNA abundance and mRNA expression levels. Conclusion The expression of marker genes of adipogenesis and adipocyte metabolism, including the master regulator PPARγ, was down-regulated in subcutaneous adipose tissue from this patient. Adiponectin mRNA expression was also reduced but leptin mRNA levels were unaltered. Markers of local inflammatory status were unaltered. Expression of genes related to mitochondrial function was reduced despite unaltered levels of mitochondrial DNA. It is concluded that adipogenic and mitochondrial gene expression is impaired in adipose tissue in this patient with acquired partial lipodystrophy.
National Oceanic and Atmospheric Administration, Department of Commerce — Glider oceanographic data (temperature, salinity) collected in support of the Deepwater Horizon oil spill event in the Gulf of Mexico (NODC Accession 0065238).
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0000439 includes chemical, meteorological, physical and underway - surface data collected from LILLOOET in the Coastal Waters of SE Alaska, Coral Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — This data set, 3 of 5 archived at the NODC, contains digital orthophotography. The data set presents information that represented conditions for the specified...
National Oceanic and Atmospheric Administration, Department of Commerce — This data set, 2 of 5 archived at the NODC, contains digital orthophotography. The data set presents information that represented conditions for the specified...
National Oceanic and Atmospheric Administration, Department of Commerce — This data set, 4 of 5 archived at the NODC, contains digital orthophotography. The data set presents information that represented conditions for the specified...
National Oceanic and Atmospheric Administration, Department of Commerce — This data set, 1 of 5 archived at the NODC, contains digital orthophotography. The data set presents information that represented conditions for the specified...
National Oceanic and Atmospheric Administration, Department of Commerce — This data set, 5 of 5 archived at the NODC, contains digital orthophotography. The data set presents information that represented conditions for the specified...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113534 includes chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1990-05-15 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117712 includes Surface underway, chemical, meteorological and physical data collected from THALASSA in the North Atlantic Ocean from 2012-08-19 to...
Bakhoum, Niokhor; Galiana, Antoine; Le Roux, Christine; Kane, Aboubacry; Duponnois, Robin; Ndoye, Fatou; Fall, Dioumacor; Noba, Kandioura; Sylla, Samba Ndao; Diouf, Diégane
2015-04-01
Acacia senegal and Acacia seyal are small, deciduous legume trees, most highly valued for nitrogen fixation and for the production of gum arabic, a commodity of international trade since ancient times. Symbiotic nitrogen fixation by legumes represents the main natural input of atmospheric N2 into ecosystems which may ultimately benefit all organisms. We analyzed the nod and nif symbiotic genes and symbiotic properties of root-nodulating bacteria isolated from A. senegal and A. seyal in Senegal. The symbiotic genes of rhizobial strains from the two Acacia species were closed to those of Mesorhizobium plurifarium and grouped separately in the phylogenetic trees. Phylogeny of rhizobial nitrogen fixation gene nifH was similar to those of nodulation genes (nodA and nodC). All A. senegal rhizobial strains showed identical nodA, nodC, and nifH gene sequences. By contrast, A. seyal rhizobial strains exhibited different symbiotic gene sequences. Efficiency tests demonstrated that inoculation of both Acacia species significantly affected nodulation, total dry weight, acetylene reduction activity (ARA), and specific acetylene reduction activity (SARA) of plants. However, these cross-inoculation tests did not show any specificity of Mesorhizobium strains toward a given Acacia host species in terms of infectivity and efficiency as stated by principal component analysis (PCA). This study demonstrates that large-scale inoculation of A. senegal and A. seyal in the framework of reafforestation programs requires a preliminary step of rhizobial strain selection for both Acacia species.
Directory of Open Access Journals (Sweden)
Yu Zhang
2016-12-01
Full Text Available Rice blast disease is one of the most destructive rice diseases worldwide. The pi21 gene confers partial and durable resistance to Magnaporthe oryzae. However, little is known regarding the molecular mechanisms of resistance mediated by the loss-of-function of Pi21. In this study, comparative transcriptome profiling of the Pi21-RNAi transgenic rice line and Nipponbare with M. oryzae infection at different time points (0, 12, 24, 48, and 72 hpi were investigated using RNA sequencing. The results generated 43,222 unique genes mapped to the rice genome. In total, 1,109 differentially expressed genes (DEGs were identified between the Pi21-RNAi line and Nipponbare with M. oryzae infection, with 103, 281, 209, 69, and 678 DEGs at 0, 12, 24, 48, and 72 hpi, respectively. Functional analysis showed that most of the DEGs were involved in metabolism, transport, signaling, and defense. Among the genes assigned to plant–pathogen interaction, we identified 43 receptor kinase genes associated with pathogen-associated molecular pattern recognition and calcium ion influx. The expression levels of brassinolide-insensitive 1, flagellin sensitive 2 and elongation factor Tu receptor, ethylene (ET biosynthesis and signaling genes, were higher in the Pi21-RNAi line than Nipponbare. This suggested that there was a more robust PTI response in Pi21-RNAi plants and that ET signaling was important to rice blast resistance. We also identified 53 transcription factor genes, including WRKY, NAC, DOF, and ERF families that show differential expression between the two genotypes. This study highlights possible candidate genes that may serve a function in the partial rice blast resistance mediated by the loss-of-function of Pi21 and increase our understanding of the molecular mechanisms involved in partial resistance against M. oryzae.
Taravat, Elham; Zebarjadi, Alireza; Kahrizi, Danial; Yari, Kheirollah
2015-05-01
Among the essential amino acids, phenylalanine, tryptophan, and tyrosine are aromatic amino acids which are synthesized by the shikimate pathway in plants and bacteria. Herbicide glyphosate can inhibit the biosynthesis of these amino acids. So, identification of the gene tolerant to glyphosate is very important. It has been shown that the common reed or Phragmites australis Cav. (Poaceae) is relatively tolerant to glyphosate. The aim of the current research is identification, cloning, sequencing, and registering of partial aro A gene of the common reed P. australis. The partial aro A gene of common reed (P. australis) was cloned in Escherichia coli and the amino acid sequence was identified/determined for the first time. This is the first report for isolation, cloning, and sequencing of a part of aro A gene from the common reed. A 670 bp fragment including two introns (86 bp and 289 bp) was obtained. The open reading frame (ORF) region in part of gene was encoded for 98 amino acids. Alignment showed high similarity among this region with Zea mays (L.) (Poaceae) (94.6%), Eleusine indica L. Gaertn (Poaceae) (94.2%), and Zoysia japonica Steud. (Poaceae) (94.2%). The alignment of amino acid sequence of the investigated part of the gene showed a homology with aro A from several other plants. This conserved region forms the enzyme active site. The alignment results of nucleotide and amino acid residues with related sequences showed that there are some differences among them. The relative glyphosate tolerance in the common reed may be related to these differences.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115177 includes chemical, discrete sample, physical and profile data collected from METEOR in the South Atlantic Ocean from 1994-03-29 to 1994-05-11...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0114447 includes biological, chemical, discrete sample, physical and profile data collected from USCGC POLAR SEA in the North Greenland Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113530 includes chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1989-05-11 to...
Globicatella sanguinis bacteraemia identified by partial 16S rRNA gene sequencing
DEFF Research Database (Denmark)
Abdul-Redha, Rawaa Jalil; Balslew, Ulla; Christensen, Jens Jørgen
2007-01-01
Globicatella sanguinis is a gram-positive coccus, resembling non-haemolytic streptococci. The organism has been isolated infrequently from normally sterile sites of humans. Three isolates obtained by blood culture could not be identified by Rapid 32 ID Strep, but partial sequencing of the 16S r......RNA gene revealed the identity of the isolated bacteria, and supplementary biochemical tests confirmed the species identification. The cases histories illustrate the dilemma of finding relevant, newly recognized, opportunistic pathogens and the identification achievement (s) that can be obtained by using...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113522 includes chemical, discrete sample, physical and profile data collected from ATLANTIS II in the North Atlantic Ocean from 1989-04-20 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115171 includes chemical, discrete sample, physical and profile data collected from THOMAS WASHINGTON in the South Pacific Ocean from 1991-07-16 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115151 includes chemical, discrete sample, physical and profile data collected from NATHANIEL B. PALMER in the Indian Ocean from 1996-05-03 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115013 includes chemical, discrete sample, physical and profile data collected from AKADEMIK IOFFE in the South Pacific Ocean from 1992-02-14 to...
In Vivo Gene Therapy of Hemophilia B: Sustained Partial Correction in Factor IX-Deficient Dogs
Kay, Mark A.; Rothenberg, Steven; Landen, Charles N.; Bellinger, Dwight A.; Leland, Frances; Toman, Carol; Finegold, Milton; Thompson, Arthur R.; Read, M. S.; Brinkhous, Kenneth M.; Woo, Savio L. C.
1993-10-01
The liver represents a model organ for gene therapy. A method has been developed for hepatic gene transfer in vivo by the direct infusion of recombinant retroviral vectors into the portal vasculature, which results in the persistent expression of exogenous genes. To determine if these technologies are applicable for the treatment of hemophilia B patients, preclinical efficacy studies were done in a hemophilia B dog model. When the canine factor IX complementary DNA was transduced directly into the hepatocytes of affected dogs in vivo, the animals constitutively expressed low levels of canine factor IX for more than 5 months. Persistent expression of the clotting. factor resulted in reductions of whole blood clotting and partial thromboplastin times of the treated animals. Thus, long-term treatment of hemophilia B patients may be feasible by direct hepatic gene therapy in vivo.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0114999 includes chemical, discrete sample, physical and profile data collected from JAMES CLARK ROSS in the South Atlantic Ocean from 1995-03-20 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116069 includes chemical, discrete sample, physical and profile data collected from NATHANIEL B. PALMER in the South Pacific Ocean from 1997-01-13 to...
Ma, W; Zhang, T-F; Lu, P; Lu, S H
2014-01-01
Breast cancer is categorized into two broad groups: estrogen receptor positive (ER+) and ER negative (ER-) groups. Previous study proposed that under trastuzumab-based neoadjuvant chemotherapy, tumor initiating cell (TIC) featured ER- tumors response better than ER+ tumors. Exploration of the molecular difference of these two groups may help developing new therapeutic strategies, especially for ER- patients. With gene expression profile from the Gene Expression Omnibus (GEO) database, we performed partial least squares (PLS) based analysis, which is more sensitive than common variance/regression analysis. We acquired 512 differentially expressed genes. Four pathways were found to be enriched with differentially expressed genes, involving immune system, metabolism and genetic information processing process. Network analysis identified five hub genes with degrees higher than 10, including APP, ESR1, SMAD3, HDAC2, and PRKAA1. Our findings provide new understanding for the molecular difference between TIC featured ER- and ER+ breast tumors with the hope offer supports for therapeutic studies.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115022 includes chemical, discrete sample, physical and profile data collected from METEOR in the South Atlantic Ocean from 1991-02-10 to 1991-03-23...
Directory of Open Access Journals (Sweden)
Wang Hehe
2012-08-01
Full Text Available Abstract Background Phytophthora sojae is the primary pathogen of soybeans that are grown on poorly drained soils. Race-specific resistance to P. sojae in soybean is gene-for-gene, although in many areas of the US and worldwide there are populations that have adapted to the most commonly deployed resistance to P. sojae ( Rps genes. Hence, this system has received increased attention towards identifying mechanisms and molecular markers associated with partial resistance to this pathogen. Several quantitative trait loci (QTL have been identified in the soybean cultivar ‘Conrad’ that contributes to the expression of partial resistance to multiple P. sojae isolates. Results In this study, two of the Conrad QTL on chromosome 19 were dissected through sequence and expression analysis of genes in both resistant (Conrad and susceptible (‘Sloan’ genotypes. There were 1025 single nucleotide polymorphisms (SNPs in 87 of 153 genes sequenced from Conrad and Sloan. There were 304 SNPs in 54 genes sequenced from Conrad compared to those from both Sloan and Williams 82, of which 11 genes had SNPs unique to Conrad. Eleven of 19 genes in these regions analyzed with qRT-PCR had significant differences in fold change of transcript abundance in response to infection with P. sojae in lines with QTL haplotype from the resistant parent compared to those with the susceptible parent haplotype. From these, 8 of the 11 genes had SNPs in the upstream, untranslated region, exon, intron, and/or downstream region. These 11 candidate genes encode proteins potentially involved in signal transduction, hormone-mediated pathways, plant cell structural modification, ubiquitination, and basal resistance. Conclusions These findings may indicate a complex defense network with multiple mechanisms underlying these two soybean QTL conferring resistance to P. sojae. SNP markers derived from these candidate genes can contribute to fine mapping of QTL and marker assisted breeding for
National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains NODC station data spanning from January 1935 to December 1978. The data set contains quality controlled station data profiles for standard...
Delayed CTD data submitted by INIDEP ranging from 11/26/1984 - 10/16/1989 (NODC Accession 0039468)
National Oceanic and Atmospheric Administration, Department of Commerce — CTD data were collected in the South Atlantic aboard the Oca Balda from 26 November 1984 to 16 October 1989. These data were submitted to NODC by the INSTITUTO...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113885 includes chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1990-06-12 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115156 includes chemical, discrete sample, physical and profile data collected from KNORR in the North Pacific Ocean and South Pacific Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115021 includes chemical, discrete sample, physical and profile data collected from METEOR in the South Atlantic Ocean and South Pacific Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117495 includes Surface underway, biological, chemical, meteorological and physical data collected from Drifting Buoy in the Indian Ocean, South...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115153 includes chemical, meteorological, physical and underway - surface data collected from AURORA AUSTRALIS in the Great Australian Bight, Indian...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112931 includes chemical, meteorological, physical and underway - surface data collected from SKOGAFOSS in the Gulf of St. Lawrence, Labrador Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115256 includes chemical, meteorological, physical and underway - surface data collected from JAMES CLARK ROSS in the Arctic Ocean, Barents Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108120 includes biological, chemical, meteorological, physical and underway - surface data collected from ROGER REVELLE in the Bay of Bengal and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115018 includes chemical, discrete sample, physical and profile data collected from KNORR in the South Pacific Ocean and Tasman Sea from 1992-05-02...
Wu, Sa; Zhang, Xin; Li, Zhi-Ming; Shi, Yan-Xia; Huang, Jia-Jia; Xia, Yi; Yang, Hang; Jiang, Wen-Qi
2013-01-01
Post-transplant lymphoproliferative disorder (PTLD) is a common complication of therapeutic immunosuppression after organ transplantation. Gene expression profile facilitates the identification of biological difference between Epstein-Barr virus (EBV) positive and negative PTLDs. Previous studies mainly implemented variance/regression analysis without considering unaccounted array specific factors. The aim of this study is to investigate the gene expression difference between EBV positive and negative PTLDs through partial least squares (PLS) based analysis. With a microarray data set from the Gene Expression Omnibus database, we performed PLS based analysis. We acquired 1188 differentially expressed genes. Pathway and Gene Ontology enrichment analysis identified significantly over-representation of dysregulated genes in immune response and cancer related biological processes. Network analysis identified three hub genes with degrees higher than 15, including CREBBP, ATXN1, and PML. Proteins encoded by CREBBP and PML have been reported to be interact with EBV before. Our findings shed light on expression distinction of EBV positive and negative PTLDs with the hope to offer theoretical support for future therapeutic study.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108071 includes chemical, discrete sample, physical and profile data collected from KNORR in the South Pacific Ocean from 2005-08-21 to 2005-10-06...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115005 includes chemical, discrete sample, physical and profile data collected from KNORR in the Caribbean Sea and North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115014 includes chemical, discrete sample, physical and profile data collected from MELVILLE in the North Atlantic Ocean and South Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0072200 contains temperature, salinity, nutrients, and meteorological data collected from 1926 to 1991 aboard multiple platforms in Caspian Sea.
Directory of Open Access Journals (Sweden)
Masataka Hirasaki
Full Text Available Predominant transcriptional subnetworks called Core, Myc, and PRC modules have been shown to participate in preservation of the pluripotency and self-renewality of embryonic stem cells (ESCs. Epiblast stem cells (EpiSCs are another cell type that possesses pluripotency and self-renewality. However, the roles of these modules in EpiSCs have not been systematically examined to date. Here, we compared the average expression levels of Core, Myc, and PRC module genes between ESCs and EpiSCs. EpiSCs showed substantially higher and lower expression levels of PRC and Core module genes, respectively, compared with those in ESCs, while Myc module members showed almost equivalent levels of average gene expression. Subsequent analyses revealed that the similarity in gene expression levels of the Myc module between these two cell types was not just overall, but striking similarities were evident even when comparing the expression of individual genes. We also observed equivalent levels of similarity in the expression of individual Myc module genes between induced pluripotent stem cells (iPSCs and partial iPSCs that are an unwanted byproduct generated during iPSC induction. Moreover, our data demonstrate that partial iPSCs depend on a high level of c-Myc expression for their self-renewal properties.
Armas-Capote, Natalia; Pérez-Yépez, Juan; Martínez-Hidalgo, Pilar; Garzón-Machado, Víctor; Del Arco-Aguilar, Marcelino; Velázquez, Encarna; León-Barrios, Milagros
2014-03-01
Cicer canariense is a threatened perennial wild chickpea endemic to the Canary Islands. In this study, rhizobia that nodulate this species in its natural habitats on La Palma (Canary Islands) were characterised. The genetic diversity and phylogeny were estimated by RAPD profiles, 16S-RFLP analysis and sequencing of the rrs, recA, glnII and nodC genes. 16S-RFLP grouped the isolates within the Mesorhizobium genus and distinguished nine different ribotypes. Four branches included minority ribotypes (3-5 isolates), whereas another five contained the predominant ribotypes that clustered with reference strains of M. tianshanense/M. gobiense/M. metallidurans, M. caraganae, M. opportunistum, M. ciceri and M. tamadayense. The sequences confirmed the RFLP groupings but resolved additional internal divergence within the M. caraganae group and outlined several potential novel species. The RAPD profiles showed a high diversity at the infraspecific level, except in the M. ciceri group. The nodC phylogeny resolved three symbiotic lineages. A small group of isolates had sequences identical to those of symbiovar ciceri and were only detected in M. ciceri isolates. Another group of sequences represented a novel symbiotic lineage that was associated with two particular chromosomal backgrounds. However, nodC sequences closely related to symbiovar loti predominated in most isolates, and they were detected in several chromosomal backgrounds corresponding to up to nine Mesorhizobium lineages. The results indicated that C. canariense is a promiscuous legume that can be nodulated by several rhizobial species and symbiotypes, which means it will be important to determine the combination of core and symbiotic genes that produce the most effective symbiosis. Copyright © 2013 Elsevier GmbH. All rights reserved.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115001 includes chemical, discrete sample, physical, profile and underway - surface data collected from METEOR in the North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109925 includes biological, chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Caribbean Sea,...
Debiève, F; Depoix, C; Gruson, D; Hubinont, C
2013-09-01
Timely regulated changes in oxygen partial pressure are important for placental formation. Disturbances could be responsible for pregnancy-related diseases like preeclampsia and intrauterine growth restriction. We aimed to (i) determine the effect of oxygen partial pressure on cytotrophoblast differentiation; (ii) measure mRNA expression and protein secretion from genes associated with placental angiogenesis; and (iii) determine the reversibility of these effects at different oxygen partial pressures. Term cytotrophoblasts were incubated at 21% and 2.5% O2 for 96 hr, or were switched between the two oxygen concentrations after 48 hr. Real-time PCR and enzyme-linked immunosorbent assays (ELISAs) were used to evaluate cell fusion and differentiation, measuring transcript levels for those genes involved in cell fusion and placental angiogenesis, including VEGF, PlGF, VEGFR1, sVEGFR1, sENG, INHA, and GCM1. Cytotrophoblasts underwent fusion and differentiation in 2.5% O2 . PlGF expression was inhibited while sVEGFR1 expression increased. VEGF and sENG mRNA expressions increased in 2.5% compared to 21% O2 , but no protein was detected in the cell supernatants. Finally, GCM1 mRNA expression increased during trophoblast differentiation at 21% O2 , but was inhibited at 2.5% O2 . These mRNA expression effects were reversed by returning the cells to 21% O2 . Thus, low-oxygen partial pressure does not inhibit term-cytotrophoblast cell fusion and differentiation in vitro. Lowering the oxygen partial pressure from 21% to 2.5% caused normal-term trophoblasts to reversibly modify their expression of genes associated with placental angiogenesis. This suggests that modifications observed in pregnancy diseases such as preeclampsia or growth retardation are probably due to an extrinsic effect on trophoblasts. Copyright © 2013 Wiley Periodicals, Inc.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115686 includes biological, chemical, discrete sample, physical and profile data collected from LE NOROIT in the North Atlantic Ocean and South...
Directory of Open Access Journals (Sweden)
H Mirhendi
2008-12-01
Full Text Available Background: Approximately 85-90% of malaria infections in Iran are attributed to Plasmodium vivax, while little is known about the genetic of the parasite and its strain types in this region. This study was designed and performed for describing genetic characteristics of Plasmodium vivax population of Iran based on the merozoite surface protein-3α gene sequence. Methods: Through a descriptive study we analyzed partial P. vivax merozoite surface protein-3α gene sequences from 17 clinical P. vivax isolates collected from malarious areas of Iran. Genomic DNA was extracted by Q1Aamp® DNA blood mini kit, amplified through nested PCR for a partial nucleotide sequence of PvMSP-3 gene in P. vivax. PCR-amplified products were sequenced with an ABI Prism Perkin-Elmer 310 sequencer machine and the data were analyzed with clustal W software. Results: Analysis of PvMSP-3 gene sequences demonstrated extensive polymorphisms, but the sequence identity between isolates of same types was relatively high. We identified specific insertions and deletions for the types A, B and C variants of P. vivax in our isolates. In phylogenetic comparison of geographically separated isolates, there was not a significant geographical branching of the parasite populations. Conclusion: The highly polymorphic nature of isolates suggests that more investigations of the PvMSP-3 gene are needed to explore its vaccine potential.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113558 includes chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Atlantic Ocean, North Greenland Sea and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113539 includes chemical, discrete sample, physical and profile data collected from EDWIN LINK in the North Atlantic Ocean and South Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108098 includes chemical, discrete sample, physical and profile data collected from HESPERIDES in the North Atlantic Ocean from 2003-04-08 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113560 includes chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Atlantic Ocean, North Greenland Sea and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117699 includes Surface underway, chemical, meteorological and physical data collected from NOAA Ship RONALD H. BROWN in the North Atlantic Ocean and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108227 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0114448 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116411 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108228 includes biological, chemical, meteorological, physical and underway - surface data collected from MARION DUFRESNE in the Indian Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115002 includes chemical, discrete sample, meteorological, physical, profile and underway - surface data collected from KNORR in the North Atlantic...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112929 includes chemical, meteorological, physical and underway - surface data collected from SKOGAFOSS in the North Atlantic Ocean and Stellwagen...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115173 includes chemical, discrete sample, meteorological, physical, profile and underway - surface data collected from METEOR in the South Atlantic...
National Oceanic and Atmospheric Administration, Department of Commerce — This data set consists of an MS Word file which documents and summarizes data previously submitted as MS Excel spreadsheets to the NOAA data centers, NODC ACCESSION...
National Oceanic and Atmospheric Administration, Department of Commerce — Since 1990, biota of the coral reef ecosystems have been annualy monitored at stations located at and in the vicinity of the Waianae Ocean Sewage Outfall. NODC...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0081032 includes biological, chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Caribbean Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109924 includes biological, chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Caribbean Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115181 includes chemical, meteorological, physical and underway - surface data collected from SOUTHERN SURVEYOR in the Bass Strait, Coral Sea, Great...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108084 includes chemical, discrete sample, meteorological, physical, profile and underway - surface data collected from MIRAI in the Coral Sea, North...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0114477 includes Surface underway, chemical, meteorological and physical data collected from ANTARES in the North Atlantic Ocean and South Atlantic...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0081033 includes biological, chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Caribbean Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0081044 includes chemical, meteorological, physical and underway - surface data collected from KEIFU MARU in the East China Sea (Tung Hai), North...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116979 includes Surface underway, chemical, meteorological and physical data collected from NOAA Ship RONALD H. BROWN in the South Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117697 includes Surface underway, chemical, meteorological and physical data collected from NOAA Ship GORDON GUNTER in the Gulf of Mexico from...
Lucas-Herald, A.; S. Bertelloni (Silvano); A. Juul (Anders); J. Bryce (Jillian); Jiang, J.; M. Rodie (Martina); R. Sinnott (Richard); Boroujerdi, M.; Lindhardt Johansen, M.; O. Hiort (Olaf); P-M. Holterhus (Paul-Martin); M.L. Cools (Martine); Guaragna-Filho, G.; Guerra-Junior, G.; N. Weintrob (Naomi); S.E. Hannema (Sabine); S.L.S. Drop (Stenvert); T. Guran (Tulay); F. Darendeliler (Feyza); A. Nordenström (Anna); I.A. Hughes (Ieuan A.); Acerini, C.; Tadokoro-Cuccaro, R.; S.F. Ahmed (Faisal)
2016-01-01
textabstractBackground: In boys with suspected partial androgen insensitivity syndrome (PAIS), systematic evidence that supports the long-term prognostic value of identifying a mutation in the androgen receptor gene (AR) is lacking. Objective: To assess the clinical characteristics and long-term
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117690 includes Surface underway, chemical, meteorological and physical data collected from KNORR in the North Atlantic Ocean from 2011-06-28 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117493 includes Surface underway, chemical, meteorological and physical data collected from USS BOLD in the Gulf of Mexico from 2006-06-06 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115765 includes chemical, meteorological, physical and underway - surface data collected from CAPE HATTERAS in the Gulf of Mexico from 2009-01-09 to...
National Oceanic and Atmospheric Administration, Department of Commerce — Temperature and salinity measurements taken from elephant seals in the Antarctic, Coastal South Indian Ocean and other locations from 2004 to 2006 (NODC Accession...
Kuzmin, I V; Hughes, G J; Rupprecht, C E
2006-08-01
Partial nucleoprotein (N) gene sequences of the rhabdoviruses Obodhiang (OBOV), Kotonkon (KOTV), Rochambeau (RBUV), Kern canyon (KCV), Mount Elgon bat (MEBV), Kolongo (KOLV) and Sandjimba (SJAV) were generated and their phylogenetic positions within the family Rhabdoviridae were determined. Both OBOV and KOTV were placed within the genus Ephemerovirus. RBUV was joined to the same cluster, but more distantly. MEBV and KCV were grouped into a monophyletic cluster (putative genus) with Oita virus (OITAV). These three viruses, originating from different regions of the world, were all isolated from insectivorous bats and may be specific for these mammals. African avian viruses KOLV and SJAV were joined to each other and formed another clade at the genus level. Further, they were grouped with the recently characterized rhabdovirus Tupaia virus (TRV). Although the genetic distance was great, the grouping was supported by consistent bootstrap values. This observation suggests that viruses of this group may be distributed widely in the Old World. Non-synonymous/synonymous substitution ratio estimations (dN/dS) using a partial N gene fragment (241 codons) for the three rhabdovirus genera revealed contrasting patterns of evolution, where dN/dS values follow the pattern Ephemerovirus > Vesiculovirus > Lyssavirus. The magnitude of this ratio corresponds well with the number of negatively selected codons. The accumulation of dS appears evenly distributed along the gene fragment for all three genera. These estimations demonstrated clearly that lyssaviruses are subjected to the strongest constraints against amino acid substitutions, probably related to their particular niche and unique pathobiology.
National Oceanic and Atmospheric Administration, Department of Commerce — Water depth and other data from 18 January 1975 to 23 February 1975. Data were collected by National Ocean Data Center (NODC) as part of the International Decade of...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
Sönksen, Ute Wolff; Christensen, Jens Jørgen; Nielsen, Lisbeth; Hesselbjerg, Annemarie; Hansen, Dennis Schrøder; Bruun, Brita
2010-12-31
Taxonomy and identification of fastidious Gram negatives are evolving and challenging. We compared identifications achieved with the Vitek 2 Neisseria-Haemophilus (NH) card and partial 16S rRNA gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious Gram negative bacteria. Seventy-five strains represented 21 of the 26 taxa included in the Vitek 2 NH database and 25 strains represented related species not included in the database. Of the 100 strains, 31 were the type strains of the species. Vitek 2 NH identification results: 48 of 75 database strains were correctly identified, 11 strains gave `low discrimination´, seven strains were unidentified, and nine strains were misidentified. Identification of 25 non-database strains resulted in 14 strains incorrectly identified as belonging to species in the database. Partial 16S rRNA gene sequence analysis results: For 76 strains phenotypic and sequencing identifications were identical, for 23 strains the sequencing identifications were either probable or possible, and for one strain only the genus was confirmed. Thus, the Vitek 2 NH system identifies most of the commonly occurring species included in the database. Some strains of rarely occurring species and strains of non-database species closely related to database species cause problems. Partial 16S rRNA gene sequence analysis performs well, but does not always suffice, additional phenotypical characterization being useful for final identification.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112932 includes chemical, meteorological, physical and underway - surface data collected from SKOGAFOSS in the North Atlantic Ocean, North Greenland...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115605 includes Surface underway, chemical, meteorological and physical data collected from METEOR in the English Channel, Indian Ocean, North...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112941 includes chemical, meteorological, physical and underway - surface data collected from POLARSTERN in the North Atlantic Ocean and South...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115226 includes chemical, meteorological, physical and underway - surface data collected from Atlantic Companion in the Inner Sea - West Coast...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112930 includes chemical, meteorological, physical and underway - surface data collected from SKOGAFOSS in the North Atlantic Ocean, North Greenland...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115708 includes chemical, meteorological, physical and underway - surface data collected from SOUTHERN SURVEYOR in the Coral Sea, Great Australian...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 9700096 contains temperature and salinity profile data from bottle casts collected aboard the USCGC Seneca in the Grand Banks of Newfoundland and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115180 includes chemical, meteorological, physical and underway - surface data collected from L'ASTROLABE in the Indian Ocean, South Pacific Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109931 includes biological, chemical, meteorological, physical and underway - surface data collected from NOAA Ship RONALD H. BROWN in the Caribbean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115295 includes chemical, meteorological, physical and underway - surface data collected from SOUTHERN SURVEYOR in the Coral Sea, Indian Ocean, South...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117500 includes Surface underway, chemical and physical data collected from USS BOLD in the Gulf of Mexico from 2007-05-02 to 2007-08-24. These data...
Association between NLPR1, NLPR3, and P2X7R Gene Polymorphisms with Partial Seizures
Directory of Open Access Journals (Sweden)
Haidong Wang
2017-01-01
Full Text Available Objectives. Clinical and experimental evidence has clarified that the inflammatory processes within the brain play a pivotal role in the pathophysiology of seizures and epilepsy. Inflammasomes and P2X7 purinergic receptor (P2X7R are important mediators during the inflammatory process. Therefore, we investigated the possible association between partial seizures and inflammasomes NLPR1, NLRP3, and P2X7R gene polymorphisms in the present study. Method. A total of 163 patients and 201 health controls were enrolled in this study and polymorphisms of NLPR1, NLRP3, and P2X7R genes were detected using polymerase chain reaction- (PCR- ligase detection reaction method. Result. The frequency of rs878329 (G>C genotype with C (CG + CC was significantly lower among patients with partial seizures relative to controls (OR = 2.033, 95% CI = 1.290–3.204, p=0.002 for GC + CC versus GG. Intriguingly, we found that the significant difference of rs878329 (G>C genotype and allele frequency only existed among males (OR = 2.542, 95% CI = 1.344–4.810, p=0.004 for GC + CC versus GG, while there was no statistically significant difference among females. However, no significant results were presented for the genotype distributions of rs8079034, rs4612666, rs10754558, rs2027432, rs3751143, and rs208294 polymorphisms between patients and controls. Conclusion. Our study demonstrated the potentially significant role of NLRP1 rs878329 (G>C in developing susceptibility to the partial seizures in a Chinese Han population.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0100072 includes chemical, physical and time series data collected from MOORINGS in the North Pacific Ocean and Olympic Coast National Marine...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109932 includes chemical, meteorological, physical and underway - surface data collected from XUE LONG in the Arctic Ocean, Beaufort Sea and Bering...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117499 includes Surface underway, chemical, meteorological and physical data collected from L'ASTROLABE in the Indian Ocean, South Pacific Ocean and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108229 includes chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Bay of Fundy, Caribbean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0083189 includes chemical, physical and underway - surface data collected from NATHANIEL B. PALMER in the Arctic Ocean, Beaufort Sea, Bering Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0083196 includes chemical, physical and underway - surface data collected from LAURENCE M. GOULD in the Caribbean Sea, North Pacific Ocean, South...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108094 includes chemical, meteorological, physical and underway - surface data collected from NOAA Ship RONALD H. BROWN in the North Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108230 includes chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Bay of Fundy, Caribbean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108231 includes chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Bay of Fundy, Caribbean...
Zhou, Luo; Zhang, Mengqi; Long, Hongyu; Long, Lili; Xie, Yuanyuan; Liu, Zhaoqian; Kang, Jin; Chen, Qihua; Feng, Li; Xiao, Bo
2015-11-15
Drug resistance in epilepsy is common despite many antiepileptic drugs (AEDs) available for treatment. The development of drug resistant epilepsy may be a result of multiple factors. Several previous studies reported that the major vault protein (MVP) was significantly increased in epileptogenic brain tissues resected from patients with partial-onset seizures, indicating the possible involvement of MVP in drug resistance. In this article, we aimed to identify the association between single nucleotide polymorphisms (SNPs) of MVP gene and drug resistance of partial epilepsy in a Chinese Han population. A total of 510 patients with partial-onset seizures and 206 healthy controls were recruited. Among the patients, 222 were drug resistant and 288 were responsive. The selection of tagging SNPs was based on the Hapmap database and Haploview software and the genotyping was conducted on the Sequenom MassARRAY iPLEX platform. For the selected loci rs12149746, rs9938630 and rs4788186 in the MVP gene, there was no significant difference in allele or genotype distribution between the drug resistant and responsive groups, or between all of the patients and healthy controls. Linkage disequilibrium between any two loci was detected but there was no significant difference in haplotype frequency between the drug resistant and responsive groups. Our results suggest that MVP genetic polymorphisms and haplotypes may not be associated with drug resistance of partial epilepsy in the Chinese Han population. Copyright © 2015 Elsevier B.V. All rights reserved.
National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, tritium and other measurements found in datasets XBT and CTD taken from the URANIA (Call sign IQSU) in the Mediteranean from 2001 to 2002 (NODC...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108235 includes chemical, meteorological, physical and underway - surface data collected from XUE LONG in the Bali Sea, Celebes Sea, East China Sea...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109934 includes chemical, meteorological, physical and underway - surface data collected from NOAA Ship McARTHUR II in the Coastal Waters of SE...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115589 includes chemical, meteorological, physical and underway - surface data collected from KNORR in the Andaman Sea or Burma Sea, Arabian Sea, Bay...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0083197 includes chemical, physical and underway - surface data collected from HEALY in the Arctic Ocean, Beaufort Sea, Bering Sea, Coastal Waters of...
National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, salinity, and meteorology data received at NODC on 05/02/03 by Igor Smolyar from the personal library of Dr. Aleksey Zuyev, Murmansk Branch of the...
National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, salinity, oxygen and other profile data received at NODC on 09/09/04 by Sydney Levitus from the Institute of Ocean Sciences (Sidney, B.C.), digitized...
National Oceanic and Atmospheric Administration, Department of Commerce — The Peoples Republic of China, Institute of Marine Scientific and Technological Information provided NODC with historical oceanographic data. Two original tapes...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115172 includes chemical, discrete sample, meteorological, physical, profile and underway - surface data collected from THOMAS G. THOMPSON in the...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117725 includes Surface underway, chemical, meteorological and physical data collected from POLARSTERN in the English Channel, North Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117715 includes Surface underway, biological, chemical, meteorological and physical data collected from NOAA Ship MALCOLM BALDRIGE in the Banda Sea,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117502 includes Surface underway, chemical, meteorological and physical data collected from NOAA Ship MILLER FREEMAN in the Bering Sea, Coastal...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117704 includes Surface underway, chemical, meteorological and physical data collected from NOAA Ship GORDON GUNTER in the Florida Keys National...
National Oceanic and Atmospheric Administration, Department of Commerce — Ten data files containing 2,738 data records were submitted by Martien Baars to NODC/Ocean Climate Laboratory from the Netherlands Indian Ocean Programme. These...
National Oceanic and Atmospheric Administration, Department of Commerce — Source: Temperature, chlorophyll and other profile data received at NODC on 04/01/03 by Todd O'Brien from "Fukuda, Y., M. Ohno, M. Fukuchi, 1986 "Surface Chlorophyll...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109928 includes chemical, meteorological, physical and underway - surface data collected from EXPLORER OF THE SEAS in the Caribbean Sea and North...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108088 includes chemical, meteorological, physical and underway - surface data collected from L'ATALANTE in the Gulf of Guinea, North Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117337 includes Surface underway, chemical, meteorological and physical data collected from MONTE OLIVIA and Rio Blanco in the English Channel, North...
Ghebremedhin, B; Layer, F; König, W; König, B
2008-03-01
The analysis of 16S rRNA gene sequences has been the technique generally used to study the evolution and taxonomy of staphylococci. However, the results of this method do not correspond to the results of polyphasic taxonomy, and the related species cannot always be distinguished from each other. Thus, new phylogenetic markers for Staphylococcus spp. are needed. We partially sequenced the gap gene (approximately 931 bp), which encodes the glyceraldehyde-3-phosphate dehydrogenase, for 27 Staphylococcus species. The partial sequences had 24.3 to 96% interspecies homology and were useful in the identification of staphylococcal species (F. Layer, B. Ghebremedhin, W. König, and B. König, J. Microbiol. Methods 70:542-549, 2007). The DNA sequence similarities of the partial staphylococcal gap sequences were found to be lower than those of 16S rRNA (approximately 97%), rpoB (approximately 86%), hsp60 (approximately 82%), and sodA (approximately 78%). Phylogenetically derived trees revealed four statistically supported groups: S. hyicus/S. intermedius, S. sciuri, S. haemolyticus/S. simulans, and S. aureus/epidermidis. The branching of S. auricularis, S. cohnii subsp. cohnii, and the heterogeneous S. saprophyticus group, comprising S. saprophyticus subsp. saprophyticus and S. equorum subsp. equorum, was not reliable. Thus, the phylogenetic analysis based on the gap gene sequences revealed similarities between the dendrograms based on other gene sequences (e.g., the S. hyicus/S. intermedius and S. sciuri groups) as well as differences, e.g., the grouping of S. arlettae and S. kloosii in the gap-based tree. From our results, we propose the partial sequencing of the gap gene as an alternative molecular tool for the taxonomical analysis of Staphylococcus species and for decreasing the possibility of misidentification.
Ghebremedhin, B.; Layer, F.; König, W.; König, B.
2008-01-01
The analysis of 16S rRNA gene sequences has been the technique generally used to study the evolution and taxonomy of staphylococci. However, the results of this method do not correspond to the results of polyphasic taxonomy, and the related species cannot always be distinguished from each other. Thus, new phylogenetic markers for Staphylococcus spp. are needed. We partially sequenced the gap gene (∼931 bp), which encodes the glyceraldehyde-3-phosphate dehydrogenase, for 27 Staphylococcus species. The partial sequences had 24.3 to 96% interspecies homology and were useful in the identification of staphylococcal species (F. Layer, B. Ghebremedhin, W. König, and B. König, J. Microbiol. Methods 70:542-549, 2007). The DNA sequence similarities of the partial staphylococcal gap sequences were found to be lower than those of 16S rRNA (∼97%), rpoB (∼86%), hsp60 (∼82%), and sodA (∼78%). Phylogenetically derived trees revealed four statistically supported groups: S. hyicus/S. intermedius, S. sciuri, S. haemolyticus/S. simulans, and S. aureus/epidermidis. The branching of S. auricularis, S. cohnii subsp. cohnii, and the heterogeneous S. saprophyticus group, comprising S. saprophyticus subsp. saprophyticus and S. equorum subsp. equorum, was not reliable. Thus, the phylogenetic analysis based on the gap gene sequences revealed similarities between the dendrograms based on other gene sequences (e.g., the S. hyicus/S. intermedius and S. sciuri groups) as well as differences, e.g., the grouping of S. arlettae and S. kloosii in the gap-based tree. From our results, we propose the partial sequencing of the gap gene as an alternative molecular tool for the taxonomical analysis of Staphylococcus species and for decreasing the possibility of misidentification. PMID:18174295
National Oceanic and Atmospheric Administration, Department of Commerce — Entry contains old Japanese data submitted to NODC. Water depth and other physical profile data were collected using over a dozen different Japanese ships and other...
National Oceanic and Atmospheric Administration, Department of Commerce — This entry contains tidal information for Chesapeake Bay. Data was submitted by Saul Berkman, NOS Tides Branch, Oceanographic Division. These data are in NODC...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0081046 includes chemical, meteorological, physical and underway - surface data collected from Ryofu Maru II in the Bismarck Sea, East China Sea (Tung...
H.T. Brüggenwirth (Hennie); A.L.M. Boehmer (Annemie); S. Ramnarain; M.C. Verleun-Mooijman; D.P.E. Satijn (David); J. Trapman (Jan); J.A. Grootegoed (Anton); A.O. Brinkmann (Albert)
1997-01-01
textabstractIn the coding part and the intron-exon boundaries of the androgen-receptor gene of a patient with partial androgen insensitivity, no mutation was found. The androgen receptor of this patient displayed normal ligand-binding parameters and migrated as a
National Oceanic and Atmospheric Administration, Department of Commerce — One year data in 1988 was collected from Gulf of Guinea using ship named Kakadiamaa by Fisheries Institute, Tema, Ghana. NODC coding forms were used to report data...
Yang, Mingxing; Li, Xiumin; Li, Zhibin; Ou, Zhimin; Liu, Ming; Liu, Suhuan; Li, Xuejun; Yang, Shuyu
2013-01-01
DNA microarray analysis is characterized by obtaining a large number of gene variables from a small number of observations. Cluster analysis is widely used to analyze DNA microarray data to make classification and diagnosis of disease. Because there are so many irrelevant and insignificant genes in a dataset, a feature selection approach must be employed in data analysis. The performance of cluster analysis of this high-throughput data depends on whether the feature selection approach chooses the most relevant genes associated with disease classes. Here we proposed a new method using multiple Orthogonal Partial Least Squares-Discriminant Analysis (mOPLS-DA) models and S-plots to select the most relevant genes to conduct three-class disease classification and prediction. We tested our method using Golub's leukemia microarray data. For three classes with subtypes, we proposed hierarchical orthogonal partial least squares-discriminant analysis (OPLS-DA) models and S-plots to select features for two main classes and their subtypes. For three classes in parallel, we employed three OPLS-DA models and S-plots to choose marker genes for each class. The power of feature selection to classify and predict three-class disease was evaluated using cluster analysis. Further, the general performance of our method was tested using four public datasets and compared with those of four other feature selection methods. The results revealed that our method effectively selected the most relevant features for disease classification and prediction, and its performance was better than that of the other methods.
Directory of Open Access Journals (Sweden)
Kollmar Martin
2008-02-01
Full Text Available Abstract Background Alternative splicing of mutually exclusive exons is an important mechanism for increasing protein diversity in eukaryotes. The insect Mhc (myosin heavy chain gene produces all different muscle myosins as a result of alternative splicing in contrast to most other organisms of the Metazoa lineage, that have a family of muscle genes with each gene coding for a protein specialized for a functional niche. Results The muscle myosin heavy chain genes of 22 species of the Arthropoda ranging from the waterflea to wasp and Drosophila have been annotated. The analysis of the gene structures allowed the reconstruction of an ancient muscle myosin heavy chain gene and showed that during evolution of the arthropods introns have mainly been lost in these genes although intron gain might have happened in a few cases. Surprisingly, the genome of Aedes aegypti contains another and that of Culex pipiens quinquefasciatus two further muscle myosin heavy chain genes, called Mhc3 and Mhc4, that contain only one variant of the corresponding alternative exons of the Mhc1 gene. Mhc3 transcription in Aedes aegypti is documented by EST data. Mhc3 and Mhc4 inserted in the Aedes and Culex genomes either by gene duplication followed by the loss of all but one variant of the alternative exons, or by incorporation of a transcript of which all other variants have been spliced out retaining the exon-intron structure. The second and more likely possibility represents a new type of a 'partially' processed pseudogene. Conclusion Based on the comparative genomic analysis of the alternatively spliced arthropod muscle myosin heavy chain genes we propose that the splicing process operates sequentially on the transcript. The process consists of the splicing of the mutually exclusive exons until one exon out of the cluster remains while retaining surrounding intronic sequence. In a second step splicing of introns takes place. A related mechanism could be responsible for
National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, Salinity and meteorology data digitized at NODC on 05/02/03, received by Igor Smolyar, from the personal library papers of Dr. Aleksey Zuyev, Murmansk...
National Oceanic and Atmospheric Administration, Department of Commerce — Format and other information for these data may only be available in analog form at this time. Please contact Services.NODC@noaa.gov for additional information.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0110259 includes biological, chemical, meteorological, physical and underway - surface data collected from SANTA LUCIA and Santa Maria in the...
Genomic instability is a hallmark of human cancer, and results in widespread somatic copy number alterations. We used a genome-scale shRNA viability screen in human cancer cell lines to systematically identify genes that are essential in the context of particular copy-number alterations (copy-number associated gene dependencies). The most enriched class of copy-number associated gene dependencies was CYCLOPS (Copy-number alterations Yielding Cancer Liabilities Owing to Partial losS) genes, and spliceosome components were the most prevalent.
National Oceanic and Atmospheric Administration, Department of Commerce — The sound velocity and other data in this accession has been processed by NODC from a tape submitted by the originator containing Inverted Echo Sounder data from the...
National Oceanic and Atmospheric Administration, Department of Commerce — The 4 km Pathfinder effort at the National Oceanic and Atmospheric Administration (NOAA) National Oceanographic Data Center (NODC) and the University of Miami's...
DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy
Directory of Open Access Journals (Sweden)
Deming Li
2014-06-01
Full Text Available Epidermal growth factor (EGF, a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF was down-regulated in total during liver regeneration. We think that the rat EGF promoter region is regulated by variation in DNA methylation during liver regeneration.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112324 includes Surface underway, chemical, meteorological, physical and time series data collected from NATHANIEL B. PALMER and ROGER REVELLE in the...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126498 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108093 includes chemical, meteorological, physical and underway - surface data collected from NOAA Ship RONALD H. BROWN in the Gulf of Guinea, Gulf...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123055 contains raw underway navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA Ship Thomas...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124303 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117838 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125087 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125267 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
Energy Technology Data Exchange (ETDEWEB)
Young, M.; Antonarakis, S.E. [Univ. of Geneva (Switzerland); Inaba, Hiroshi [Tokyo Medical College (Japan)] [and others
1997-03-01
Although the molecular defect in patients in a Japanese family with mild to moderately severe hemophilia A was a deletion of a single nucleotide T within an A{sub 8}TA{sub 2} sequence of exon 14 of the factor VIII gene, the severity of the clinical phenotype did not correspond to that expected of a frameshift mutation. A small amount of functional factor VIII protein was detected in the patient`s plasma. Analysis of DNA and RNA molecules from normal and affected individuals and in vitro transcription/translation suggested a partial correction of the molecular defect, because of the following: (i) DNA replication/RNA transcription errors resulting in restoration of the reading frame and/or (ii) {open_quotes}ribosomal frameshifting{close_quotes} resulting in the production of normal factor VIII polypeptide and, thus, in a milder than expected hemophilia A. All of these mechanisms probably were promoted by the longer run of adenines, A{sub 10} instead of A{sub 8}TA{sub 2}, after the delT. Errors in the complex steps of gene expression therefore may partially correct a severe frameshift defect and ameliorate an expected severe phenotype. 36 refs., 6 figs.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123614 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125346 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113243 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118685 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0099244 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125757 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117012 contains raw underway meteorological, navigational, optical and physical data logged by the Scientific Computer System (SCS) aboard NOAA Ship...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118187 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122499 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123054 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125582 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
Directory of Open Access Journals (Sweden)
Harsi Dewantari Kusumaningrum
2016-08-01
Full Text Available The choice of primer used in 16S rRNA sequencing for identification of Staphylococcus species found in food is important. This study aimed to characterize Staphylococcus aureus isolates by partial sequencing based on 16S rRNA gene employing primers 16sF, 63F or 1387R. The isolates were isolated from milk, egg dishes and chicken dishes and selected based on the presence of sea gene that responsible for formation of enterotoxin-A. Antibiotic susceptibility of the isolates towards six antibiotics was also tested. The use of 16sF resulted generally in higher identity percentage and query coverage compared to the sequencing by 63F or 1387R. BLAST results of all isolates, sequenced by 16sF, showed 99% homology to complete genome of four S. aureus strains, with different characteristics on enterotoxin production and antibiotic resistance. Considering that all isolates were carrying sea gene, indicated by the occurence of 120 bp amplicon after PCR amplification using primer SEA1/SEA2, the isolates were most in agreeing to S. aureus subsp. aureus ST288. This study indicated that 4 out of 8 selected isolates were resistant towards streptomycin. The 16S rRNA gene sequencing using 16sF is useful for identification of S. aureus. However, additional analysis such as PCR employing specific gene target, should give a valuable supplementary information, when specific characteristic is expected.
National Oceanic and Atmospheric Administration, Department of Commerce — The Chinese National Oceanographic Data Center provided NODC with 13 tapes of CTD, XBT, station data, chlorophyll, primary productivity and vessel report data...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0083626 includes underway chemical and physical data collected from COLUMBUS ISELIN, ENDEAVOR, GYRE, OCEANUS, and SEWARD JOHNSON in the North Atlantic...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119414 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123613 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113448 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113487 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0121197 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0084476 includes underway meteorological and oceanographic chemical and physical data collected aboard the Celtic Explorer in North Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124186 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0121982 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
Li, Ye Long; Dai, Xin Ren; Yue, Xun; Gao, Xin-Qi; Zhang, Xian Sheng
2014-10-01
Maize 1,491 small secreted peptides were identified, which were classified according to the character of peptide sequences. Partial SSP gene expressions in reproductive tissues were determined by qRT-PCR. Small secreted peptides (SSPs) are important cell-cell communication messengers in plants. Most information on plant SSPs come from Arabidopsis thaliana and Oryza sativa, while little is known about the SSPs of other grass species such as maize (Zea mays). In this study, we identified 1,491 SSP genes from maize genomic sequences. These putative SSP genes were distributed throughout the ten maize chromosomes. Among them, 611 SSPs were classified into 198 superfamilies according to their conserved domains, and 725 SSPs with four or more cysteines at their C-termini shared similar cysteine arrangements with their counterparts in other plant species. Moreover, the SSPs requiring post-translational modification, as well as defensin-like (DEFL) proteins, were identified. Further, the expression levels of 110 SSP genes were analyzed in reproductive tissues, including male flower, pollen, silk, and ovary. Most of the genes encoding basal-layer antifungal peptide-like, small coat proteins-like, thioredoxin-like proteins, γ-thionins-like, and DEFL proteins showed high expression levels in the ovary and male flower compared with their levels in silk and mature pollen. The rapid alkalinization factor-like genes were highly expressed only in the mature ovary and mature pollen, and pollen Ole e 1-like genes showed low expression in silk. The results of this study provide basic information for further analysis of SSP functions in the reproductive process of maize.
González-Guerra, José Luis; Castilla-Cortazar, Inma; Aguirre, Gabriel A; Muñoz, Úrsula; Martín-Estal, Irene; Ávila-Gallego, Elena; Granado, Miriam; Puche, Juan E; García-Villalón, Ángel Luis
2017-01-01
Circulating levels of IGF-1 may decrease under several circumstances like ageing, metabolic syndrome, and advanced cirrhosis. This reduction is associated with insulin resistance, dyslipidemia, progression to type 2 diabetes, and increased risk for cardiovascular diseases. However, underlying mechanisms between IGF-1 deficiency and cardiovascular disease remain elusive. The specific aim of the present work was to study whether the partial IGF-1 deficiency influences heart and/or coronary circulation, comparing vasoactive factors before and after of ischemia-reperfusion (I/R). In addition, histology of the heart was performed together with cardiac gene expression for proteins involved in structure and function (extracellular matrix, contractile proteins, active peptides); carried out using microarrays, followed by RT-qPCR confirmation of the three experimental groups. IGF-1 partial deficiency is associated to a reduction in contractility and angiotensin II sensitivity, interstitial fibrosis as well as altered expression pattern of genes involved in extracellular matrix proteins, calcium dynamics, and cardiac structure and function. Although this work is descriptive, it provides a clear insight of the impact that partial IGF-1 deficiency on the heart and establishes this experimental model as suitable for studying cardiac disease mechanisms and exploring therapeutic options for patients under IGF-1 deficiency conditions.
Directory of Open Access Journals (Sweden)
José Luis González-Guerra
Full Text Available Circulating levels of IGF-1 may decrease under several circumstances like ageing, metabolic syndrome, and advanced cirrhosis. This reduction is associated with insulin resistance, dyslipidemia, progression to type 2 diabetes, and increased risk for cardiovascular diseases. However, underlying mechanisms between IGF-1 deficiency and cardiovascular disease remain elusive. The specific aim of the present work was to study whether the partial IGF-1 deficiency influences heart and/or coronary circulation, comparing vasoactive factors before and after of ischemia-reperfusion (I/R. In addition, histology of the heart was performed together with cardiac gene expression for proteins involved in structure and function (extracellular matrix, contractile proteins, active peptides; carried out using microarrays, followed by RT-qPCR confirmation of the three experimental groups. IGF-1 partial deficiency is associated to a reduction in contractility and angiotensin II sensitivity, interstitial fibrosis as well as altered expression pattern of genes involved in extracellular matrix proteins, calcium dynamics, and cardiac structure and function. Although this work is descriptive, it provides a clear insight of the impact that partial IGF-1 deficiency on the heart and establishes this experimental model as suitable for studying cardiac disease mechanisms and exploring therapeutic options for patients under IGF-1 deficiency conditions.
International Nuclear Information System (INIS)
Osanya, A.; Majiwa, P.A.O.; Kinyanjui, P.W.
2006-01-01
Specific oligonucleotide primers based on conserved nucleotide sequences of 18s ribisomal RNA (18s rRNA) gene of Trypanosoma brucei, Leishmania donovani, Triponema aequale and Lagenidium gigantum have been designed and used in the ploymerase chain reaction (PCR) to amplify genomic DNA from four different clones each representing a different genotypic group of T. congolence. PCR products of approximately 1Kb were generated using as template DNA from each of the trypanosomes. The PCR products cross-hybridized with genomic DNA from T.brucei, T. simiae and the four genotypes of T.congolense implying significant sequence homology of 18S rRNA gene among trypanosomes. The nucleotide sequence of a segment of the PCR products were determined by direct sequencing to provide partial nucleotide sequence of the 18s rRNA gene in each T.congolense genotypic group. The sequences obtained together with those that have been published for T.brucei reveals that although most regions show inter and intra species nucleotide identity, there are several sites where deletions, insertions and base changes have occured in nucleotide sequence of of T.brucei and the four genotypes of T.congolense.(author)
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0038589 contains all the data the CLIVAR and Carbon Hydrographic Data Office (CCHDO) assembled and processed through 2017-07-05. This Accession...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0100079 includes chemical, time series and underway - surface data collected from MOORINGS in the North Pacific Ocean and South Pacific Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — Data from the CLIVAR-Carbon Hydrographic Office (CCHDO) were acquired by NODC. Cruises in this accession include: A16N_Leg1, A16N_Leg2, A16S, A20, A22, P02, and...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125711 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115902 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0120616 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113248 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113519 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125266 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123056 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125709 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125710 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125265 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125584 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115702 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125666 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125758 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125919 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115052 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116135 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
Partial Androgen Insensitivity Syndrome Presenting with Gynecomastia
Directory of Open Access Journals (Sweden)
Sung Won Lee
2015-06-01
Full Text Available Gynecomastia is a benign enlargement of the male breast caused by the proliferation of glandular breast tissue. Determining the various causes of gynecomastia such as physiological causes, drugs, systemic diseases, and endocrine disorders is important. Androgen insensitivity syndrome (AIS is a rare endocrine disorder presenting with gynecomastia and is a disorder of male sexual differentiation caused by mutations within the androgen receptor gene. All individuals with AIS have the 46 XY karyotype, although AIS phenotypes can be classified as mild, partial or complete and can differ among both males and females including ambiguous genitalia or infertility in males. We experienced a case of partial AIS presenting with gynecomastia and identified the androgen receptor gene mutation.
Directory of Open Access Journals (Sweden)
Berhanu Ayalew
2010-08-01
Full Text Available Abstract Background Newcastle disease (ND, caused by Newcastle disease virus (NDV, is a highly contagious disease of birds and has been one of the major causes of economic losses in the poultry industry. Despite routine vaccination programs, sporadic cases have occasionally occurred in the country and remain a constant threat to commercial poultry. Hence, the present study was aimed to characterize NDV isolates obtained from clinical cases in various locations of Malaysia between 2004 and 2007 based on sequence and phylogenetic analysis of partial F gene and C-terminus extension length of HN gene. Results The coding region of eleven NDV isolates fusion (F gene and carboxyl terminal region of haemagglutinin-neuraminidase (HN gene including extensions were amplified by reverse transcriptase PCR and directly sequenced. All the isolates have shown to have non-synonymous to synonymous base substitution rate ranging between 0.081 - 0.264 demonstrating presence of negative selection. Analysis based on F gene showed the characterized isolates possess three different types of protease cleavage site motifs; namely 112RRQKRF117, 112RRRKRF117 and 112GRQGRL117 and appear to show maximum identities with isolates in the region such as cockatoo/14698/90 (Indonesia, Ch/2000 (China, local isolate AF2240 indicating the high similarity of isolates circulating in the South East Asian countries. Meanwhile, one of the isolates resembles commonly used lentogenic vaccine strains. On further characterization of the HN gene, Malaysian isolates had C-terminus extensions of 0, 6 and 11 amino acids. Analysis of the phylogenetic tree revealed that the existence of three genetic groups; namely, genotype II, VII and VIII. Conclusions The study concluded that the occurrence of three types of NDV genotypes and presence of varied carboxyl terminus extension lengths among Malaysian isolates incriminated for sporadic cases.
Berhanu, Ayalew; Ideris, Aini; Omar, Abdul R; Bejo, Mohd Hair
2010-08-08
Newcastle disease (ND), caused by Newcastle disease virus (NDV), is a highly contagious disease of birds and has been one of the major causes of economic losses in the poultry industry. Despite routine vaccination programs, sporadic cases have occasionally occurred in the country and remain a constant threat to commercial poultry. Hence, the present study was aimed to characterize NDV isolates obtained from clinical cases in various locations of Malaysia between 2004 and 2007 based on sequence and phylogenetic analysis of partial F gene and C-terminus extension length of HN gene. The coding region of eleven NDV isolates fusion (F) gene and carboxyl terminal region of haemagglutinin-neuraminidase (HN) gene including extensions were amplified by reverse transcriptase PCR and directly sequenced. All the isolates have shown to have non-synonymous to synonymous base substitution rate ranging between 0.081 - 0.264 demonstrating presence of negative selection. Analysis based on F gene showed the characterized isolates possess three different types of protease cleavage site motifs; namely 112RRQKRF117, 112RRRKRF117 and 112GRQGRL117 and appear to show maximum identities with isolates in the region such as cockatoo/14698/90 (Indonesia), Ch/2000 (China), local isolate AF2240 indicating the high similarity of isolates circulating in the South East Asian countries. Meanwhile, one of the isolates resembles commonly used lentogenic vaccine strains. On further characterization of the HN gene, Malaysian isolates had C-terminus extensions of 0, 6 and 11 amino acids. Analysis of the phylogenetic tree revealed that the existence of three genetic groups; namely, genotype II, VII and VIII. The study concluded that the occurrence of three types of NDV genotypes and presence of varied carboxyl terminus extension lengths among Malaysian isolates incriminated for sporadic cases.
National Oceanic and Atmospheric Administration, Department of Commerce — This data file consists of chlorophyll and other parameter built from existing NODC files F004, water physics/ chemistry, F029, primary productivity, as well as...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119096 contains raw underway biological, meteorological, navigational, physical, profile and time series data logged by the Scientific Computer...
DEFF Research Database (Denmark)
Abdul-Redha, Rawaa Jalil; Kemp, Michael; Bangsborg, Jette M
2010-01-01
Streptococci, enterococci and Streptococcus-like bacteria are frequent etiologic agents of infective endocarditis and correct species identification can be a laboratory challenge. Viridans streptococci (VS) not seldomly cause contamination of blood cultures. Vitek 2 and partial sequencing of the 16......S rRNA gene were applied in order to compare the results of both methods. STRAINS ORIGINATED FROM TWO GROUPS OF PATIENTS: 149 strains from patients with infective endocarditis and 181 strains assessed as blood culture contaminants. Of the 330 strains, based on partial 16S rRNA gene sequencing......-agreeing identifications with the two methods with respect to allocation to the same VS group. Non-agreeing species identification mostly occurred among strains in the contaminant group, while for endocarditis strains notably fewer disagreeing results were observed.Only 67 of 150 strains in the mitis group strains...
Directory of Open Access Journals (Sweden)
Barbaro Michela
2013-01-01
Full Text Available Abstract Variegate porphyria (VP is an autosomal dominantly inherited hepatic porphyria. The genetic defect in the PPOX gene leads to a partial defect of protoporphyrinogen oxidase, the penultimate enzyme of heme biosynthesis. Affected individuals can develop cutaneous symptoms in sun-exposed areas of the skin and/or neuropsychiatric acute attacks. The identification of the genetic defect in VP families is of crucial importance to detect the carrier status which allows counseling to prevent potentially life threatening neurovisceral attacks, usually triggered by factors such as certain drugs, alcohol or fasting. In a total of 31 Swedish VP families sequence analysis had identified a genetic defect in 26. In the remaining five families an extended genetic investigation was necessary. After the development of a synthetic probe set, MLPA analysis to screen for single exon deletions/duplications was performed. We describe here, for the first time, two partial deletions within the PPOX gene detected by MLPA analysis. One deletion affects exon 5 and 6 (c.339-197_616+320del1099 and has been identified in four families, most probably after a founder effect. The other extends from exon 5 to exon 9 (c.339-350_987+229del2609 and was found in one family. We show that both deletions are mediated by Alu repeats. Our findings emphasize the usefulness of MLPA analysis as a complement to PPOX gene sequencing analysis for comprehensive genetic diagnostics in patients with VP.
Partial duplication of the APBA2 gene in chromosome 15q13 corresponds to duplicon structures
Directory of Open Access Journals (Sweden)
Kesterson Robert A
2003-04-01
Full Text Available Abstract Background Chromosomal abnormalities affecting human chromosome 15q11-q13 underlie multiple genomic disorders caused by deletion, duplication and triplication of intervals in this region. These events are mediated by highly homologous segments of DNA, or duplicons, that facilitate mispairing and unequal cross-over in meiosis. The gene encoding an amyloid precursor protein-binding protein (APBA2 was previously mapped to the distal portion of the interval commonly deleted in Prader-Willi and Angelman syndromes and duplicated in cases of autism. Results We show that this gene actually maps to a more telomeric location and is partially duplicated within the broader region. Two highly homologous copies of an interval containing a large 5' exon and downstream sequence are located ~5 Mb distal to the intact locus. The duplicated copies, containing the first coding exon of APBA2, can be distinguished by single nucleotide sequence differences and are transcriptionally inactive. Adjacent to APBA2 maps a gene termed KIAA0574. The protein encoded by this gene is weakly homologous to a protein termed X123 that in turn maps adjacent to APBA1 on 9q21.12; APBA1 is highly homologous to APBA2 in the C-terminal region and is distinguished from APBA2 by the N-terminal region encoded by this duplicated exon. Conclusion The duplication of APBA2 sequences in this region adds to a complex picture of different low copy repeats present across this region and elsewhere on the chromosome.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125756 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126660 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122396 contains raw underway meteorological, navigational, optical, physical, profile, time series and trawl data logged by the Scientific Computer...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118545 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116096 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118186 contains raw underway meteorological, navigational, optical, physical, profile, time series and trawl data logged by the Scientific Computer...
National Oceanic and Atmospheric Administration, Department of Commerce — This data is a special submission to NODC containing wave data during Hurricane Andrew August 25-26, 1992. This data file contains 24 bursts spaced at 2 hour...
Partial Correlation Matrix Estimation using Ridge Penalty Followed by Thresholding and Reestimation
2014-01-01
Summary Motivated by the problem of construction gene co-expression network, we propose a statistical framework for estimating high-dimensional partial correlation matrix by a three-step approach. We first obtain a penalized estimate of a partial correlation matrix using ridge penalty. Next we select the non-zero entries of the partial correlation matrix by hypothesis testing. Finally we reestimate the partial correlation coefficients at these non-zero entries. In the second step, the null distribution of the test statistics derived from penalized partial correlation estimates has not been established. We address this challenge by estimating the null distribution from the empirical distribution of the test statistics of all the penalized partial correlation estimates. Extensive simulation studies demonstrate the good performance of our method. Application on a yeast cell cycle gene expression data shows that our method delivers better predictions of the protein-protein interactions than the Graphic Lasso. PMID:24845967
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117812 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119156 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116842 contains raw underway meteorological, navigational, optical, physical, profile, time series and trawl data logged by the Scientific Computer...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0119200 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0100064 includes chemical, physical, time series and underway - surface data collected from METEOR, POSEIDON, TALIARTE and VICTOR HENSEN in the North...
Araud, Tanguy; Graw, Sharon; Berger, Ralph; Lee, Michael; Neveu, Estele; Bertrand, Daniel; Leonard, Sherry
2011-10-15
The human α7 neuronal nicotinic acetylcholine receptor gene (CHRNA7) is a candidate gene for schizophrenia and an important drug target for cognitive deficits in the disorder. Activation of the α7*nAChR, results in opening of the channel and entry of mono- and divalent cations, including Ca(2+), that presynaptically participates to neurotransmitter release and postsynaptically to down-stream changes in gene expression. Schizophrenic patients have low levels of α7*nAChR, as measured by binding of the ligand [(125)I]-α-bungarotoxin (I-BTX). The structure of the gene, CHRNA7, is complex. During evolution, CHRNA7 was partially duplicated as a chimeric gene (CHRFAM7A), which is expressed in the human brain and elsewhere in the body. The association between a 2bp deletion in CHRFAM7A and schizophrenia suggested that this duplicate gene might contribute to cognitive impairment. To examine the putative contribution of CHRFAM7A on receptor function, co-expression of α7 and the duplicate genes was carried out in cell lines and Xenopus oocytes. Expression of the duplicate alone yielded protein expression but no functional receptor and co-expression with α7 caused a significant reduction of the amplitude of the ACh-evoked currents. Reduced current amplitude was not correlated with a reduction of I-BTX binding, suggesting the presence of non-functional (ACh-silent) receptors. This hypothesis is supported by a larger increase of the ACh-evoked current by the allosteric modulator 1-(5-chloro-2,4-dimethoxy-phenyl)-3-(5-methyl-isoxazol-3-yl)-urea (PNU-120596) in cells expressing the duplicate than in the control. These results suggest that CHRFAM7A acts as a dominant negative modulator of CHRNA7 function and is critical for receptor regulation in humans. Copyright © 2011 Elsevier Inc. All rights reserved.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123337 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125618 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
Pseudo Boolean Programming for Partially Ordered Genomes
Angibaud, Sébastien; Fertin, Guillaume; Thévenin, Annelyse; Vialette, Stéphane
Comparing genomes of different species is a crucial problem in comparative genomics. Different measures have been proposed to compare two genomes: number of common intervals, number of adjacencies, number of reversals, etc. These measures are classically used between two totally ordered genomes. However, genetic mapping techniques often give rise to different maps with some unordered genes. Starting from a partial order between genes of a genome, one method to find a total order consists in optimizing a given measure between a linear extension of this partial order and a given total order of a close and well-known genome. However, for most common measures, the problem turns out to be NP-hard. In this paper, we propose a (0,1)-linear programming approach to compute a linear extension of one genome that maximizes the number of common intervals (resp. the number of adjacencies) between this linear extension and a given total order. Next, we propose an algorithm to find linear extensions of two partial orders that maximize the number of adjacencies.
Schneider, Rhiannon; Rolling, William; Song, Qijian; Cregan, Perry; Dorrance, Anne E; McHale, Leah K
2016-08-11
Phytophthora root and stem rot is one of the most yield-limiting diseases of soybean [Glycine max (L.) Merr], caused by the oomycete Phytophthora sojae. Partial resistance is controlled by several genes and, compared to single gene (Rps gene) resistance to P. sojae, places less selection pressure on P. sojae populations. Thus, partial resistance provides a more durable resistance against the pathogen. In previous work, plant introductions (PIs) originating from the Republic of Korea (S. Korea) have shown to be excellent sources for high levels of partial resistance against P. sojae. Resistance to two highly virulent P. sojae isolates was assessed in 1395 PIs from S. Korea via a greenhouse layer test. Lines exhibiting possible Rps gene immunity or rot due to other pathogens were removed and the remaining 800 lines were used to identify regions of quantitative resistance using genome-wide association mapping. Sixteen SNP markers on chromosomes 3, 13 and 19 were significantly associated with partial resistance to P. sojae and were grouped into seven quantitative trait loci (QTL) by linkage disequilibrium blocks. Two QTL on chromosome 3 and three QTL on chromosome 19 represent possible novel loci for partial resistance to P. sojae. While candidate genes at QTL varied in their predicted functions, the coincidence of QTLs 3-2 and 13-1 on chromosomes 3 and 13, respectively, with Rps genes and resistance gene analogs provided support for the hypothesized mechanism of partial resistance involving weak R-genes. QTL contributing to partial resistance towards P. sojae in soybean germplasm originating from S. Korea were identified. The QTL identified in this study coincide with previously reported QTL, Rps genes, as well as novel loci for partial resistance. Molecular markers associated with these QTL can be used in the marker-assisted introgression of these alleles into elite cultivars. Annotations of genes within QTL allow hypotheses on the possible mechanisms of partial
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123094 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0127242 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126056 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125086 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124184 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123095 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123093 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113488 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0100115 includes chemical, discrete bottle, physical and time series profile data collected from Kairei, MIRAI and NATSUSHIMA in the North Pacific...
DEFF Research Database (Denmark)
Wolff Sönksen, Ute; Christensen, Jens Jørgen; Nielsen, Lisbeth
2010-01-01
Taxonomy and identification of fastidious Gram negatives are evolving and challenging. We compared identifications achieved with the Vitek 2 Neisseria-Haemophilus (NH) card and partial 16S rRNA gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic...... characterization using 100 fastidious Gram negative bacteria. Seventy-five strains represented 21 of the 26 taxa included in the Vitek 2 NH database and 25 strains represented related species not included in the database. Of the 100 strains, 31 were the type strains of the species. Vitek 2 NH identification...... results: 48 of 75 database strains were correctly identified, 11 strains gave `low discrimination´, seven strains were unidentified, and nine strains were misidentified. Identification of 25 non-database strains resulted in 14 strains incorrectly identified as belonging to species in the database. Partial...
Partial characterization of nif genes from the bacterium Azospirillum amazonense
Directory of Open Access Journals (Sweden)
D.P. Potrich
2001-09-01
Full Text Available Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the nifH and nifD genes differed from related regions of A. brasilense even in nitrogenase structural genes which are highly conserved among diazotrophic bacteria. The feasibility of the 16S ribosomal RNA gene-based PCR system for specific detection of A. amazonense was shown. Our results indicate that the PCR primers for 16S rDNA defined in this article are highly specific to A. amazonense and can distinguish this species from A. brasilense.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122504 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0120490 contains raw underway meteorological, navigational, physical and time series data logged by the Scientific Computer System (SCS) aboard NOAA...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123338 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118842 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0122397 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0127092 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0120740 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
Directory of Open Access Journals (Sweden)
Chi-Cheng Huang
2013-01-01
Full Text Available Multiclass prediction remains an obstacle for high-throughput data analysis such as microarray gene expression profiles. Despite recent advancements in machine learning and bioinformatics, most classification tools were limited to the applications of binary responses. Our aim was to apply partial least square (PLS regression for breast cancer intrinsic taxonomy, of which five distinct molecular subtypes were identified. The PAM50 signature genes were used as predictive variables in PLS analysis, and the latent gene component scores were used in binary logistic regression for each molecular subtype. The 139 prototypical arrays for PAM50 development were used as training dataset, and three independent microarray studies with Han Chinese origin were used for independent validation (n=535. The agreement between PAM50 centroid-based single sample prediction (SSP and PLS-regression was excellent (weighted Kappa: 0.988 within the training samples, but deteriorated substantially in independent samples, which could attribute to much more unclassified samples by PLS-regression. If these unclassified samples were removed, the agreement between PAM50 SSP and PLS-regression improved enormously (weighted Kappa: 0.829 as opposed to 0.541 when unclassified samples were analyzed. Our study ascertained the feasibility of PLS-regression in multi-class prediction, and distinct clinical presentations and prognostic discrepancies were observed across breast cancer molecular subtypes.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123520 contains raw underway biological, meteorological, navigational, physical, profile and time series data logged by the Scientific Computer...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
Ha, Min Jin; Sun, Wei
2014-09-01
Motivated by the problem of construction of gene co-expression network, we propose a statistical framework for estimating high-dimensional partial correlation matrix by a three-step approach. We first obtain a penalized estimate of a partial correlation matrix using ridge penalty. Next we select the non-zero entries of the partial correlation matrix by hypothesis testing. Finally we re-estimate the partial correlation coefficients at these non-zero entries. In the second step, the null distribution of the test statistics derived from penalized partial correlation estimates has not been established. We address this challenge by estimating the null distribution from the empirical distribution of the test statistics of all the penalized partial correlation estimates. Extensive simulation studies demonstrate the good performance of our method. Application on a yeast cell cycle gene expression data shows that our method delivers better predictions of the protein-protein interactions than the Graphic Lasso. © 2014, The International Biometric Society.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0125006 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124207 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124208 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123940 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124597 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123941 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124596 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0123096 contains raw underway meteorological, navigational, physical, profile and time series data logged by the Scientific Computer System (SCS)...
Wang, Tao; Ho, Gloria; Ye, Kenny; Strickler, Howard; Elston, Robert C.
2008-01-01
Genetic association studies achieve an unprecedented level of resolution in mapping disease genes by genotyping dense SNPs in a gene region. Meanwhile, these studies require new powerful statistical tools that can optimally handle a large amount of information provided by genotype data. A question that arises is how to model interactions between two genes. Simply modeling all possible interactions between the SNPs in two gene regions is not desirable because a greatly increased number of degrees of freedom can be involved in the test statistic. We introduce an approach to reduce the genotype dimension in modeling interactions. The genotype compression of this approach is built upon the information on both the trait and the cross-locus gametic disequilibrium between SNPs in two interacting genes, in such a way as to parsimoniously model the interactions without loss of useful information in the process of dimension reduction. As a result, it improves power to detect association in the presence of gene-gene interactions. This approach can be similarly applied for modeling gene-environment interactions. We compare this method with other approaches: the corresponding test without modeling any interaction, that based on a saturated interaction model, that based on principal component analysis, and that based on Tukey’s 1-df model. Our simulations suggest that this new approach has superior power to that of the other methods. In an application to endometrial cancer case-control data from the Women’s Health Initiative (WHI), this approach detected AKT1 and AKT2 as being significantly associated with endometrial cancer susceptibility by taking into account their interactions with BMI. PMID:18615621
Wang, Tao; Ho, Gloria; Ye, Kenny; Strickler, Howard; Elston, Robert C
2009-01-01
Genetic association studies achieve an unprecedented level of resolution in mapping disease genes by genotyping dense single nucleotype polymorphisms (SNPs) in a gene region. Meanwhile, these studies require new powerful statistical tools that can optimally handle a large amount of information provided by genotype data. A question that arises is how to model interactions between two genes. Simply modeling all possible interactions between the SNPs in two gene regions is not desirable because a greatly increased number of degrees of freedom can be involved in the test statistic. We introduce an approach to reduce the genotype dimension in modeling interactions. The genotype compression of this approach is built upon the information on both the trait and the cross-locus gametic disequilibrium between SNPs in two interacting genes, in such a way as to parsimoniously model the interactions without loss of useful information in the process of dimension reduction. As a result, it improves power to detect association in the presence of gene-gene interactions. This approach can be similarly applied for modeling gene-environment interactions. We compare this method with other approaches, the corresponding test without modeling any interaction, that based on a saturated interaction model, that based on principal component analysis, and that based on Tukey's one-degree-of-freedom model. Our simulations suggest that this new approach has superior power to that of the other methods. In an application to endometrial cancer case-control data from the Women's Health Initiative, this approach detected AKT1 and AKT2 as being significantly associated with endometrial cancer susceptibility by taking into account their interactions with body mass index.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116645 includes chemical, discrete sample, physical and profile data collected from POLARSTERN in the South Atlantic Ocean from 1989-09-06 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113532 includes chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1989-07-16 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113988 includes chemical, discrete sample, physical and profile data collected from ENDEAVOR in the North Atlantic Ocean from 1991-03-28 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116370 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean from 1991-09-25 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126536 contains raw underway meteorological, navigational, optical, physical, profile and time series data logged by the Scientific Computer System...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117713 includes chemical, discrete sample, physical and profile data collected from ATLANTIS II in the North Atlantic Ocean from 1981-06-12 to...
Ampomah, Osei Yaw; Mousavi, Seyed Abdollah; Lindström, Kristina; Huss-Danell, Kerstin
2017-01-01
Rhizobia nodulating native Astragalus and Oxytropis spp. in Northern Europe are not well-studied. In this study, we isolated bacteria from nodules of four Astragalus spp. and two Oxytropis spp. from the arctic and subarctic regions of Sweden and Russia. The phylogenetic analyses were performed by using sequences of three housekeeping genes (16S rRNA, rpoB and recA) and two accessory genes (nodC and nifH). The results of our multilocus sequence analysis (MLSA) of the three housekeeping genes tree showed that all the 13 isolates belonged to the genus Mesorhizobium and were positioned in six clades. Our concatenated housekeeping gene tree also suggested that the isolates nodulating Astragalus inopinatus, Astragalus frigidus, Astragalus alpinus ssp. alpinus and Oxytropis revoluta might be designated as four new Mesorhizobium species. The 13 isolates were grouped in three clades in the nodC and nifH trees. 15 N analysis suggested that the legumes in association with these isolates were actively fixing nitrogen. Copyright © 2016 Elsevier GmbH. All rights reserved.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124206 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0126876 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124185 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0124297 contains raw underway meteorological, navigational, optical, physical and time series data logged by the Scientific Computer System (SCS)...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113549 includes biological, chemical, discrete sample, physical and profile data collected from HESPERIDES in the South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113755 includes biological, chemical, discrete sample, physical and profile data collected from HESPERIDES in the South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository(GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository(GADR as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository(GADR as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository(GADR as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository(GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR) as the long-term archive for the International Global Argo Project...
Directory of Open Access Journals (Sweden)
Wissam Chiha
Full Text Available Partial transection (PT of the optic nerve is an established experimental model of secondary degeneration in the central nervous system. After a dorsal transection, retinal ganglion cells (RGCs with axons in ventral optic nerve are intact but vulnerable to secondary degeneration, whereas RGCs in dorsal retina with dorsal axons are affected by primary and secondary injuries. Using microarray, we quantified gene expression changes in dorsal and ventral retina at 1 and 7 days post PT, to characterize pathogenic pathways linked to primary and secondary degeneration.In comparison to uninjured retina Cryba1, Cryba2 and Crygs, were significantly downregulated in injured dorsal retina at days 1 and 7. While Ecel1, Timp1, Mt2A and CD74, which are associated with reducing excitotoxicity, oxidative stress and inflammation, were significantly upregulated. Genes associated with oxygen binding pathways, immune responses, cytokine receptor activity and apoptosis were enriched in dorsal retina at day 1 after PT. Oxygen binding and apoptosis remained enriched at day 7, as were pathways involved in extracellular matrix modification. Fewer changes were observed in ventral retina at day 1 after PT, most associated with the regulation of protein homodimerization activity. By day 7, apoptosis, matrix organization and signal transduction pathways were enriched. Discriminant analysis was also performed for specific functional gene groups to compare expression intensities at each time point. Altered expression of selected genes (ATF3, GFAP, Ecel1, TIMP1, Tp53 and proteins (GFAP, ECEL1 and ATF3 were semi-quantitatively assessed by qRT-PCR and immunohistochemistry respectively.There was an acute and complex primary injury response in dorsal retina indicative of a dynamic interaction between neuroprotective and neurodegenerative events; ventral retina vulnerable to secondary degeneration showed a delayed injury response. Both primary and secondary injury resulted in the
Callejón, R; Halajian, A; de Rojas, M; Marrugal, A; Guevara, D; Cutillas, C
2012-05-25
Comparative morphological, biometrical and molecular studies of Trichuris discolor isolated from Bos taurus from Spain and Iran was carried out. Furthermore, Trichuris ovis isolated from B. taurus and Capra hircus from Spain has been, molecularly, analyzed. Morphological studies revealed clear differences between T. ovis and T. discolor isolated from B. taurus but differences were not observed between populations of T. discolor isolated from different geographical regions. Nevertheless, the molecular studies based on the amplification and sequencing of the internal transcribed spacers 1 and 2 ribosomal DNA and 16S partial gene mitochondrial DNA showed clear differences between both populations of T. discolor from Spain and Iran suggesting two cryptic species. Phylogenetic studies corroborated these data. Thus, phylogenetic trees based on ITS1, ITS2 and 16S partial gene sequences showed that individuals of T. discolor from B. taurus from Iran clustered together and separated, with high bootstrap values, of T. discolor isolated from B. taurus from Spain, while populations of T. ovis from B. taurus and C. hircus from Spain clustered together but separated with high bootstrap values of both populations of T. discolor. Furthermore, a comparative phylogenetic study has been carried out with the ITS1and ITS2 sequences of Trichuris species from different hosts. Three clades were observed: the first clustered all the species of Trichuris parasitizing herbivores (T. discolor, T. ovis, Trichuris leporis and Trichuris skrjabini), the second clustered all the species of Trichuris parasitizing omnivores (Trichuris trichiura and Trichuris suis) and finally, the third clustered species of Trichuris parasitizing carnivores (Trichuris muris, Trichuris arvicolae and Trichuris vulpis). Copyright © 2011 Elsevier B.V. All rights reserved.
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115496 includes chemical, discrete sample, physical and profile data collected from Hakuho Maru in the North Pacific Ocean from 1993-05-13 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115598 includes chemical, discrete sample, physical and profile data collected from OCEAN RESEARCHER I in the Philippine Sea from 1991-06-26 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116707 includes chemical, discrete sample, physical and profile data collected from NEW HORIZON in the North Pacific Ocean from 1980-08-10 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115603 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 1999-08-13 to 1999-08-31...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115682 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 2003-06-26 to 2003-07-21...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115593 includes chemical, discrete sample, physical and profile data collected from KNORR in the South Pacific Ocean from 2006-01-30 to 2006-03-14...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115601 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 2001-06-20 to 2001-07-15...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117678 includes chemical, discrete sample, physical and profile data collected from KNORR in the North Atlantic Ocean from 1986-04-24 to 1986-05-18...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113598 includes biological, chemical, discrete sample, physical and profile data collected from TANGAROA in the Indian Ocean from 2002-02-06 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113599 includes biological, chemical, discrete sample, physical and profile data collected from TANGAROA in the Indian Ocean from 2003-02-17 to...
Osman, Wan Adnawani Meor; van Berkum, Peter; León-Barrios, Milagros; Velázquez, Encarna; Elia, Patrick; Tian, Rui; Ardley, Julie; Gollagher, Margaret; Seshadri, Rekha; Reddy, T B K; Ivanova, Natalia; Woyke, Tanja; Pati, Amrita; Markowitz, Victor; Baeshen, Mohamed N; Baeshen, Naseebh Nabeeh; Kyrpides, Nikos; Reeve, Wayne
2017-01-01
10.1601/nm.1335 Mlalz-1 (INSDC = ATZD00000000) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing nodule of Medicago laciniata (L.) Miller from a soil sample collected near the town of Guatiza on the island of Lanzarote, the Canary Islands, Spain. This strain nodulates and forms an effective symbiosis with the highly specific host M. laciniata . This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) sequencing project. Here the features of 10.1601/nm.1335 Mlalz-1 are described, together with high-quality permanent draft genome sequence information and annotation. The 6,664,116 bp high-quality draft genome is arranged in 99 scaffolds of 100 contigs, containing 6314 protein-coding genes and 74 RNA-only encoding genes. Strain Mlalz-1 is closely related to 10.1601/nm.1335 10.1601/strainfinder?urlappend=%3Fid%3DIAM+12611 T , 10.1601/nm.1334 A 321 T and 10.1601/nm.17831 10.1601/strainfinder?urlappend=%3Fid%3DORS+1407 T , based on 16S rRNA gene sequences. gANI values of ≥98.1% support the classification of strain Mlalz-1 as 10.1601/nm.1335. Nodulation of M. laciniata requires a specific nodC allele, and the nodC gene of strain Mlalz-1 shares ≥98% sequence identity with nodC of M. laciniata -nodulating 10.1601/nm.1328 strains, but ≤93% with nodC of 10.1601/nm.1328 strains that nodulate other Medicago species. Strain Mlalz-1 is unique among sequenced 10.1601/nm.1335 strains in possessing genes encoding components of a T2SS and in having two versions of the adaptive acid tolerance response lpiA-acvB operon. In 10.1601/nm.1334 strain 10.1601/strainfinder?urlappend=%3Fid%3DWSM+419, lpiA is essential for enhancing survival in lethal acid conditions. The second copy of the lpiA-acvB operon of strain Mlalz-1 has highest sequence identity (> 96%) with that of 10.1601/nm.1334 strains, which suggests genetic
National Oceanic and Atmospheric Administration, Department of Commerce — The U.S. National Oceanographic Data Center (NODC) operates the Global Argo Data Repository (GADR)as the long-term archive for the International Global Argo Project...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115587 includes chemical, discrete sample, physical and profile data collected from METEOR in the Labrador Sea and North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116566 includes chemical, discrete sample, physical and profile data collected from METEOR in the Caribbean Sea and North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115688 includes biological, chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Greenland Sea and Norwegian...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115677 includes chemical, discrete sample, physical and profile data collected from JAN MAYEN in the Norwegian Sea from 1993-04-01 to 1995-11-01 and...
DEFF Research Database (Denmark)
Wolff Sönksen, Ute; Christensen, Jens Jørgen; Nielsen, Lisbeth
2010-01-01
Taxonomy and identification of fastidious Gram negatives are evolving and challenging. We compared identifications achieved with the Vitek 2 Neisseria-Haemophilus (NH) card and partial 16S rRNA gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic...... characterization using 100 fastidious Gram negative bacteria. Seventy-five strains represented 21 of the 26 taxa included in the Vitek 2 NH database and 25 strains represented related species not included in the database. Of the 100 strains, 31 were the type strains of the species. Vitek 2 NH identification...
National Oceanic and Atmospheric Administration, Department of Commerce — Temperature, salinity, oxygen and other profile data received at NODC on 06/10/04 by Olga Baranova, digitized from "William J. Teague, Zachariah R. Hallock, Jan M....
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117714 includes biological, chemical, discrete sample, physical and profile data collected from POLARSTERN in the South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116646 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean and South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115599 includes chemical, discrete sample, physical and profile data collected from SONNE in the North Atlantic Ocean and South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113531 includes chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1989-06-12 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113576 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2000-07-19...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113573 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1998-08-18...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113574 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1998-12-05...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113575 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2000-01-17...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113570 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1998-01-21...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113571 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2003-01-23...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116643 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean and South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115585 includes chemical, discrete sample, physical and profile data collected from PELAGIA in the Bay of Biscay and North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115679 includes chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Greenland Sea and Norwegian Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115403 includes chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Greenland Sea and Norwegian Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115685 includes chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Greenland Sea and Norwegian Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117494 includes chemical, discrete sample, physical and profile data collected from L'ATALANTE in the Caribbean Sea and North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115680 includes chemical, discrete sample, physical and profile data collected from JOHAN HJORT in the North Greenland Sea and Norwegian Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112838 includes chemical, discrete sample, physical and profile data collected from BELGICA in the North Atlantic Ocean from 2006-05-31 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112839 includes chemical, discrete sample, physical and profile data collected from BELGICA in the North Atlantic Ocean from 2007-05-10 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112840 includes chemical, discrete sample, physical and profile data collected from BELGICA in the North Atlantic Ocean from 2008-05-07 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0100219 includes chemical, discrete bottle, physical and time series data collected from BOSEI MARU NO. 2, HAKUREI MARU, Hakuho Maru, Hokusei Maru,...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115602 includes chemical, discrete sample, physical and profile data collected from ARANDA in the North Atlantic Ocean and North Greenland Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113592 includes chemical, discrete sample, physical and profile data collected from PROFESSOR SIEDLECKI in the North Atlantic Ocean from 1988-11-17...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113578 includes biological, chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 2002-01-04...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113600 includes chemical, discrete sample, physical and profile data collected from THALASSA in the North Atlantic Ocean from 2001-08-23 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116409 includes chemical, discrete sample, physical and profile data collected from FRANKLIN in the Coral Sea and Tasman Sea from 1993-06-24 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116063 includes chemical, discrete sample, physical and profile data collected from NATHANIEL B. PALMER in the South Pacific Ocean from 1996-08-30 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116067 includes chemical, discrete sample, physical and profile data collected from NATHANIEL B. PALMER in the South Pacific Ocean from 1994-02-14 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116066 includes chemical, discrete sample, physical and profile data collected from NATHANIEL B. PALMER in the South Pacific Ocean from 2000-02-15 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116717 includes chemical, discrete sample, physical and profile data collected from TYRO in the Arctic Ocean and Beaufort Sea from 1996-09-13 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0109923 includes biological, chemical, meteorological, physical and underway - surface data collected from AURORA AUSTRALIS, NOAA Ship DISCOVERER,...
International Nuclear Information System (INIS)
Mufti, F.U.D.; Banaras, S.
2015-01-01
Internal control genes are the constitutive genes which maintain the basic cellular functions and regularly express in both normal and stressed conditions in living organisms. They are used in normalization of gene expression studies in comparative analysis of target genes, as their expression remains comparatively unchanged in all varied conditions. Among internal control genes, actin is considered as a candidate gene for expression studies due to its vital role in shaping cytoskeleton and plant physiology. Unfortunately most of such knowledge is limited to only model plants or crops, not much is known about important medicinal plants. Therefore, we selected seven important medicinal wild plants for molecular identification of actin gene. We used gene specific primers designed from the conserved regions of several known orthologues or homologues of actin genes from other plants. The amplified products of 370-380 bp were sequenced and submitted to GeneBank after their confirmation using different bioinformatics tools. All the novel partial sequences of putative actin genes were submitted to GeneBank (Parthenium hysterophorus (KJ774023), Fagonia indica (KJ774024), Rhazya stricta (KJ774025), Whithania coagulans (KJ774026), Capparis decidua (KJ774027), Verbena officinalis (KJ774028) and Aerva javanica (KJ774029)). The comparisons of these partial sequences by Basic Local Alignment Search Tool (BLAST) and phylogenetic trees demonstrated high similarity with known actin genes of other plants. Our findings illustrated highly conserved nature of actin gene among these selected plants. These novel partial fragments of actin genes from these wild medicinal plants can be used as internal controls for future gene expression studies of these important plants after precise validations of their stable expression in such plants. (author)
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0118793 contains oceanographic and surface meteorological data in netCDF formatted files, which follow the Climate and Forecast metadata convention...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113753 includes chemical, discrete sample, physical and profile data collected from AKADEMIK SERGEY VAVILOV in the South Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113524 includes biological, chemical, discrete sample, physical and profile data collected from DARVIN in the North Atlantic Ocean from 1991-05-01 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113759 includes chemical, discrete sample, physical and profile data collected from LE NOROIT in the North Atlantic Ocean from 1989-05-09 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113520 includes chemical, discrete sample, physical and profile data collected from L'ATALANTE in the North Atlantic Ocean from 2001-02-03 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113521 includes chemical, discrete sample, physical and profile data collected from L'ATALANTE in the North Atlantic Ocean from 2001-03-22 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117504 includes chemical, discrete sample, physical and profile data collected from MIKHAIL SOMOV in the South Atlantic Ocean from 1981-10-09 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113915 includes biological, chemical, discrete sample, physical and profile data collected from PIERRE RADISSON in the Baffin Bay from 1998-04-04 to...
Jiang, Jun; Wei, Jishu; Wu, Junli; Gao, Wentao; Li, Qiang; Jiang, Kuirong; Miao, Yi
2016-01-01
Hepatic infarcts or abscesses occur after hepatic artery interruption. We explored the mechanisms of hepatic deprivation-induced acute liver injury and determine whether partial portal vein arterialization attenuated this injury in rats. Male Sprague-Dawley rats underwent either complete hepatic arterial deprivation or partial portal vein arterialization, or both. Hepatic ischemia was evaluated using biochemical analysis, light microscopy, and transmission electron microscopy. Hepatic ATP levels, the expression of hypoxia- and inflammation-associated genes and proteins, and the expression of bile transporter genes were assessed. Complete dearterialization of the liver induced acute liver injury, as evidenced by the histological changes, significantly increased serum biochemical markers, decreased ATP content, increased expression of hypoxia- and inflammation-associated genes and proteins, and decreased expression of bile transporter genes. These detrimental changes were extenuated but not fully reversed by partial portal vein arterialization, which also attenuated ductular reaction and fibrosis in completely dearterialized rat livers. Collectively, complete hepatic deprivation causes severe liver injury, including bile infarcts and biloma formation. Partial portal vein arterialization seems to protect against acute ischemia-hypoxia-induced liver injury.
Directory of Open Access Journals (Sweden)
Jun Jiang
2016-01-01
Full Text Available Hepatic infarcts or abscesses occur after hepatic artery interruption. We explored the mechanisms of hepatic deprivation-induced acute liver injury and determine whether partial portal vein arterialization attenuated this injury in rats. Male Sprague-Dawley rats underwent either complete hepatic arterial deprivation or partial portal vein arterialization, or both. Hepatic ischemia was evaluated using biochemical analysis, light microscopy, and transmission electron microscopy. Hepatic ATP levels, the expression of hypoxia- and inflammation-associated genes and proteins, and the expression of bile transporter genes were assessed. Complete dearterialization of the liver induced acute liver injury, as evidenced by the histological changes, significantly increased serum biochemical markers, decreased ATP content, increased expression of hypoxia- and inflammation-associated genes and proteins, and decreased expression of bile transporter genes. These detrimental changes were extenuated but not fully reversed by partial portal vein arterialization, which also attenuated ductular reaction and fibrosis in completely dearterialized rat livers. Collectively, complete hepatic deprivation causes severe liver injury, including bile infarcts and biloma formation. Partial portal vein arterialization seems to protect against acute ischemia-hypoxia-induced liver injury.
Cloning and selection of reference genes for gene expression ...
African Journals Online (AJOL)
Full length mRNA sequences of Ac-β-actin and Ac-gapdh, and partial mRNA sequences of Ac-18SrRNA and Ac-ubiquitin were cloned from pineapple in this study. The four genes were tested as housekeeping genes in three experimental sets. GeNorm and NormFinder analysis revealed that β-actin was the most ...
Evolving chromosomes and gene regulatory networks
Indian Academy of Sciences (India)
Aswin
Genes under H NS control can be. (a) regulated by H NS. (b) regulated by H NS and StpA. Because backup by StpA is partial. Page 19. Gene expression level. H NS regulated xenogenes. Other genes. Page 20 ... recollect: H&NS silences highl transcribable genes. Gene expression level unilateral. Other genes epistatic ...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115586 includes chemical, discrete sample, physical and profile data collected from METEOR in the Caribbean Sea, North Atlantic Ocean and South...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113550 includes chemical, discrete sample, physical and profile data collected from HUDSON in the Labrador Sea and North Atlantic Ocean from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115763 includes chemical, discrete sample, physical and profile data collected from BELGICA, CHARLES DARWIN and METEOR in the North Atlantic Ocean...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115687 includes biological, chemical, discrete sample, physical and profile data collected from USCGC POLAR SEA in the North Greenland Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113916 includes chemical, discrete sample, physical and profile data collected from POLARSTERN in the Arctic Ocean and North Greenland Sea from...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116705 includes chemical, discrete sample, physical and profile data collected from SONNE in the Caribbean Sea, North Atlantic Ocean and South...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115320 includes chemical, discrete sample, physical and profile data collected from BOSEI MARU NO. 2 in the North Pacific Ocean from 1994-01-15 to...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113912 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 1997-05-15 to 1997-06-06...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113588 includes chemical, discrete sample, physical and profile data collected from OCEANUS in the North Atlantic Ocean from 1995-05-29 to 1995-06-03...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113914 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 1997-08-15 to 1997-09-09...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113892 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 2004-03-11 to 2004-04-13...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115015 includes chemical, discrete sample, physical and profile data collected from MARION DUFRESNE in the Indian Ocean from 1993-01-23 to 1993-03-09...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113602 includes chemical, discrete sample, physical and profile data collected from TYRO in the North Atlantic Ocean from 1990-07-01 to 1990-07-12...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113567 includes chemical, discrete sample, physical and profile data collected from KNORR in the North Atlantic Ocean from 1997-10-05 to 1997-11-19...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113551 includes chemical, discrete sample, physical and profile data collected from HUDSON in the North Atlantic Ocean from 1993-04-05 to 1993-05-14...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113946 includes chemical, discrete sample, physical and profile data collected from GAUSS in the North Atlantic Ocean from 2000-05-06 to 2000-06-06...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113911 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 1996-06-13 to 1996-07-14...
National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112762 includes chemical, discrete sample, physical and profile data collected from BELGICA in the North Sea from 2006-04-25 to 2006-05-11. These...