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Sample records for nse cyfra21-1 scc-ag

  1. Values of CEA, CYFRA21-1, NSE and SCC-Ag in pleural effusion and serum in diagnosis of lung cancer%胸水和血清CEA、CYFRA21-1、NSE、SCC-Ag测定对肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    杨铮雯; 邵润霞

    2015-01-01

    目的:探讨胸水和血清中肿瘤标志物癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)、鳞状细胞癌抗原(SCC-Ag)联合检测在肺癌中的诊断价值。方法:回顾性分析临床已确诊的101例患者胸水及血清中四项肿瘤标志物水平,应用ROC曲线建立合理的临床诊断临界值,比较各肿瘤标志物在不同病理类型肺癌表达情况。结果:肺癌组胸水和血清中四项肿瘤标志物水平均高于良性组(P <0.05),CEA对肺腺癌、NSE对小细胞肺癌、CYFRA21-1和SCC-Ag对肺鳞癌在胸水和血清中表达水平和阳性率高于其他单项检测(P <0.05);胸水和血清联合检测可提高肺癌检出率。结论:胸水和血清肿瘤标志物联合检测对肺癌检出及病理分型具有重要的参考价值。%Objective To investigate the values of combined determination of pleural effusion and serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1), neuron-specific enolase (NSE), and squamous cell carcinoma antigen (SCC-Ag) in diagnosis of lung cancer. Methods 101 patients with pleural effusion were reviewed retrospectively. Expressions of CEA, CYFRA21-1, NSE and SCC-Ag in pleural fluid and serum were detected; the optimum cut-off points resulting from the best sensitivity-specificity balance in the receiver operator characteristic (ROC) curves were constructed. The expressions of the tumor markers were compared among different pathological types of lung cancer. Results The levels of tumor markers in both pleural effusion and serum were significantly higher in patients with lung cancer than the benign group (P < 0.05). The expression levels and positive rate of CEA for lung adenocarcinoma, NSE for small cell lung cancer, and CYFRA21-1and SCC-Ag for pulmonary squamous cell carcinoma were higher than any other single detection (P < 0.05). Combined detection of the tumor markers in pleural

  2. Effect of concurrent chemoradiotherapy with cisplatin and paclitaxel on immunological function and serum CYFRA21-1 and SCC-Ag in patients with middle and advanced cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Wu-Song Tong; Song-Lin Wang; Jin-Hua Pan

    2016-01-01

    Objective:To explore the effect of concurrent chemoradiotherapy with cisplatin and paclitaxel on the immunological function and serum CYFRA21-1 and SCC-Ag in patients with middle and advanced cervical cancer.Methods:A total of 80 patients with middle and advanced cervical cancer who were admitted in our hospital from January, 2014 to January, 2016 were included in the study and randomized into the observation group and the control group with 40 cases in each group. The patients in the control group were given three-dimensional conformal radiotherapy of whole pelvic fields cassette, in a routine segmentation way, 2 GY/time, 5 time/week, DT50 GY/25F. On this basis, the patients in the observation group were given concurrent chemoradiotherapy with cisplatin and paclitaxel. The fasting venous blood before and after chemotherapy in the two groups was collected. FCM was used to detect the peripheral blood T lymphocyte subsets levels (CD3+, CD4+, and CD8+), and CD4+/CD8+ was calculated. ECLIA was used to detect the serum CYFRA21-1 and SCC-Ag levels.Results: CD3+, CD4+, and CD4+/CD8+ after treatment in the observation group were significantly elevated, and the reduced degree of CD8+ was significantly greater than that in the control group (P<0.05). The reduced degree of serum CYFRA21-1 and SCC-Ag after treatment in the observation group was significantly greater than that in the control group (P<0.05).Conclusions: Concurrent chemoradiotherapy with cisplatin and paclitaxel in the treatment of cervical cancer can effectively improve the immunological function, reduce the serum CYFRA21-1 and SCC-Ag levels, and enhance the therapeutic effect.

  3. NSE、CYFRA21-1、SCC联合检测在肺癌诊断中的应用%Application of Combined Detection of NSE, CYFRA21-1, SCC in the Diagnosis of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    马文莉

    2014-01-01

    目的:探讨与分析神经元特异性烯醇化酶物(NSE)、细胞角蛋白19片段(CYFRA21-1)、鳞状细胞癌相关抗原(SCC)联合检测在肺癌诊断中的应用效果。方法将2011年8月至2013年8月于我院接受治疗且经病理诊断为肺癌的60例患者作为观察组,选取50例良性肺疾病患者作为对照1组,同时列入30例正常、健康人作为对照2组,选用发光法分别检测所有患者血清内NSE、CYFRA21-1及SCC水平,分析不同肿瘤标志物的敏感性及特异性。结果①观察组患者血清内NSE、CYFRA21-1及SCC浓度水平明显高于正常值,且与对照1组、对照2组相比,差异具有明显统计学意义(F=21.871,P<0.05);②血清内NSE浓度水平检测以小细胞癌患者敏感度最高为75.00%,CYFRA21-1、SCC浓度水平检测则均以鳞癌患者敏感性最高,分别为44.45%、66.67%。单项肿瘤标志物浓度水平检测敏感性均<75.00%。选取三项联合检测以小细胞癌敏感度更高,为87.50%,联合检测与单项检测对比差异显著,P<0.05。结论临床上,为提高肺癌患者早期诊断的准确率,宜选用三项联合检测的方式,进而为肺癌患者的早期治疗提供指导,提升患者的生活质量。%Objective To explore and analyze the application efifcacy of Neuron speciifc enolase matter (NSE), cytokeratin 19 fragment (CYFRA21-1) and Squamous cell carcinoma antigen (SCC) in the diagnosis of lung cancer. Methods 60 cases had been pathological diagnosis as lung cancer in our hospital from August 2011 to August 2013 were selected as the observation group, 50 cases with benign pulmonary disease were selected as control 1group, also, 30 cases of normal, healthy people were selected as control 2 group. All patients were respectively detected serum NSE, CYFRA21-1

  4. 血清IGF-1及CEA、CYFRA21-1、NSE联合检测在肺癌诊治中的价值%Combination of IGF-1 with CEA, CYFRA21-1, NSE for the diagnosis and prediction of treatment response in lung cancer

    Institute of Scientific and Technical Information of China (English)

    许峰; 吴翼伟; 章斌

    2011-01-01

    目的 研究血清胰岛素样生长因子(IGF-1)及CEA、细胞角质素片断抗原(CYFRA21-1)、神经元特异性烯醇化酶(NSE)联合检测在肺癌诊治中的临床价值,以筛选理想的血清肿瘤标志物组合.方法 应用放射免疫分析法检测30名健康人、91例肺癌患者和15例肺部良性疾病患者血清IGF-1,同时应用电化学发光免疫分析法测定同一批研究对象的血清CEA、CYFRA21-1、NSE水平,采用Kruskal-Wallis单因素方差分析Mann-Whitney秩和检验进行统计学比较.用ROC曲线对各项指标的诊断效能进行分析和评价,曲线下面积(AUC)采用Wilcoxon检验分析.结果 肺癌患者的血清IGF-1水平及3种血清肿瘤标志物水平均明显高于健康人组和肺部良性疾病组,差异有统计学意义(IGF-1:χ2=26.95,P<0.001;CEA:χ2=49.11,P<0.001;CYFRA21-1:χ2=40.63,P<0.001;NSE:χ2=14.76,P<0.001),治疗后肺癌患者的IGF-1及CYFRA21-1水平比未治疗患者的IGF-1水平低,差异有统计学意义(IGF-1:χ2=5.99,P=0.014;CYFRA21-1:χ2=4.99,P=0.025).IGF-1、CEA、CYFRA21-1和NSE在特异性为95.6%(43/45)时,灵敏度分别为75.6%(34/45)、53.3%(24/45)、66.7%(30/45)和42.2%(19/45),以IGF-1最高;各指标的ROC AUC分别为0.880、0.836、0.891和0.697,以IGF-1与CYFRA21-1较高;联合检测以IGF-1+CYFRA21-1与IGF-1+CEA+CYFRA21-1较好,灵敏度分别达到95.6%(43/45)和97.8%(44/45),AUC分别为0.969和0.984.结论 血清中IGF-1、CEA、CYFRA21-1和NSE对肺癌的诊断均有一定的临床价值.IGF-1可判断疗效并进行随访.IGF-1与CEA、CYFRA21-1、NSE联合检测可显著提高肺癌诊断的灵敏度和效能,IGF-1+CYFRA21-1与IGF-1+CEA+CYFRA21-1是诊断肺癌较为理想的组合.%Objective To evaluate four tumor markers of insulin-like growth factor 1((IGF-1), CEA, cytokeratin fragment antigen 21-1 (CYFRA21-1), neuron-specific enolase (NSE) for the diagnosis and prediction of treatment response in human lung cancer. Methods Serum samples were taken from

  5. 紫杉醇联合铂类方案治疗中晚期宫颈癌患者的临床疗效及对血清 CYFRA21-1SCC-Ag 的影响%Clinical efficacy of paclitaxel combined with platinum regimen on patients with advanced cervical cancer and the effect on serum CYFRA21-1, SCC-Ag

    Institute of Scientific and Technical Information of China (English)

    高琴; 张丹丹; 白帆; 罗芳; 李芳兵

    2015-01-01

    Objective To explore the effect of paclitaxel plus platinum regimen in the treatment of advanced cervical cancer patients and effect on CYFRA 21-1, and SCC-Ag.Methods Advanced cervical cancer of 104 cases were randomly di-vided into observation group and control group with 52 cases in each group , the observation group used paclitaxel plus platinum regimen for the treatment , control group used irinotecan plus platinum regimen .Therapeutic effect , the adverse reaction and quality of life, and the serum tumor markers CYFRA21-1, SCC-Ag changes of 2 groups were observed and compared .Results The observation group's effective rate was 67.3%(35/52), which was higher than 44.2% in the control group (23/52) (χ2 =10.815, P 0.05).Conclusion Paclitaxel combined with platinum chemotherapy in the treat-ment of advanced cervix carcinoma showed exact and high effective rate , fewer adverse reactions , it can prolong the survival time of patients , improve the quality of life of patients , it is worthy of clinical application .%目的:探讨紫杉醇联合铂类方案治疗中晚期宫颈癌患者临床疗效及对CYFRA21-1、SCC-Ag的影响。方法中晚期宫颈癌患者104例随机分为观察组和对照组各52例,观察组应用紫杉醇联合铂类方案治疗,对照组应用伊立替康联合铂类方案治疗。观察2组的临床疗效、不良反应及生存质量,并对比血清肿瘤标记物CYFRA21-1、SCC-Ag变化情况。结果观察组有效率67 J.3%(35/52)高于对照组44.2%(23/52)(χ2=10.815, P <0.05)。治疗后2组CYFRA21-1、SCC-Ag均较治疗前降低[(2.12±1.03)vs.(4.56±2.07)、(1.67±1.04)vs.(3.85±1.17)]( P均<0.05),且观察组低于对照组( t =7.409、9.439, P <0.05)。治疗后,2组躯体功能、情绪功能、社会功能、疲乏、食欲减退、疼痛方面均改善(观察组t =3.759、3.964、3.738、3.316、3.670、3.057,对照组t =3.251、2.341、2.465、1.906、2

  6. CEA、CA125、CYFRA21-1和NSE在肺癌中的诊断价值%Diagnostic value of CEA, CA125, CYFRA21-1, and NSE in lung cancer

    Institute of Scientific and Technical Information of China (English)

    余才华; 马志红; 李鸿伟; 沈琦斌; 费淑祎; 刘亚萍

    2013-01-01

    目的 探讨综合运用Logistic回归和受试者工作特征曲线(ROC曲线)分析四项肿瘤标志物对肺癌的诊断价值.方法 采用放射免疫法检测1 12例原发性肺癌和74例肺良性疾病患者血清中癌胚抗原(CEA)、糖类抗原-125(CA125)、细胞角蛋白片段19(CYFRA21-1)和神经元特异性烯醇化酶(NSE)的表达水平.通过Logistic回归建立回归模型,用ROC曲线分析4项肿瘤标志物在肺癌诊断中的意义.结果 肺癌患者血清中CEA、CA125、CYFRA21-1和NSE的表达水平[4.53(2.22-11.53) ng/ml、28.97(11.39-62.10) U/ml、4.05(2.29-8.18) ng/ml、14.11(11.35-24.12)ng/ml]明显高于肺良性疾病患者[2.08(1.45-2.52) ng/ml、12.90(9.80-19.44)U/ml、1.53(1.21-2.17) ng/ml、11.38(9.07-12.80) ng/ml],差异有统计学意义(均P<0.01).通过Logistic回归建立回归方程Y=1/[1 +EXP(4.902-0.394X1-0.627X2-0.165X3)],经ROC曲线分析,新变量Y的ROC曲线下面积(AUC)为0.915 ±0.020,敏感度79.46%、特异度93.24%、准确度84.95%.结论 运用Logistic回归和ROC曲线综合分析可提高肺癌的诊断.%Objective To explore the diagnostic value of four tumor markers analyzed with Logistic regression and receiver operator characteristic (ROC) curve in patients with lung cancer.Methods The serum levels of carcinoembryonic antigen (CEA) 、carbohydrate antigen 125 (CA125),cytokeratin 19 fragment (CYFRA21-1) and neuron specific enolase (NSE) were determined by radioimmunoassay in 112 patients with lung cancer and 74 patients with benign pulmonary disease.Four tumor markers were analyzed by Logistic regression and ROC curve.Results The serum levels of CEA,CA125,CYFRA21-1 and NSE in lung cancer patients were [4.53(2.22-11.53)ng/ml,28.97 (11.39-62.10) U/ml,4.05(2.29-8.18)ng/ml,14.11 (11.35-24.12) ng/ml],respectively,which were significantly higher than those in health adults[2.08 (1.45-2.52) ng/ml,12.90 (9.80-19.44) U/ml,1.53 (1.21-2.17) ng/ml,11.38 (9.07-12.80) ng/ml] (all P < 0.01).According to

  7. 联检NSE、CYFRA21-1、D-二聚体、Fib在肺癌患者中的临床意义%Clinical significance of the combined determination of NSE, CYFRA21-1, D-Dimer, Fibrinogen for the patients with lung cancer

    Institute of Scientific and Technical Information of China (English)

    谢颖; 贾张军; 张云生

    2014-01-01

    Objective To discuss the clinical significance of the combined determination of NSE,CYFRA21-1, D-Dimer, Fibrinogen for lung cancer patients. Methods The levels of serum NSE and CYFRA21-1 were detected by electrochemical analysis in 256 lung cancer patients, 200 patients with benign lung diseases and 200 health subjects. The levels of plasma D-Dimer and Fibrinogen were detected by immunoturbidimetric assays. The relationship of NSE, CYFRA21-1, D-Dimer and Fibrinogen levels with the clinical stages of lung cancer was analyzed by statistics. Results The levels of NSE, CYFRA21-1, D-Dimer and Fibrinogen in patients with lung cancer were significantly higher than those in patients with benign lung diseases and healthy controls(PCYFRA21-1 > D-Dimer > Fibrinogen.CYFRA21-1 showed the highest sensitivity(69.0%) in squamous cellcarcinoma, and NSE showed the highest sensitivity(68.4%%) in smal celllung cancer. The combined determination of NSE, CYFRA21-1, D-Dimer, Fibrinogen might significantly improve the sensitivity for the diagnosis of lung cancer. The combined determination of NSE,CYFRA21-1, D-Dimer, Fibrinogen showed the highest sensitivity(89.8%).NSE,CYFRA21-1, D-Dimer, Fibrinogen were positively related to the clinical stages of lung cancer. With the increasing of clinical stages, the levels of NSE,CYFRA21-1, D-Dimer, Fibrinogen in Ⅲ/Ⅳ stage were significantly higher than those in Ⅰ/Ⅱ stage(correlation coefficie nt(r)=0.315,0.425,0.669,0.523,). Conclusions The combined determination of NSE, CYFRA21-1, D-Dimer, Fibrinogen has an important clinical significance in the clinical diagnosis, differentiation, monitor and clinical stage of lung cancer.%联合检测神经元特异烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA21-1)、D-二聚体(D-D)、Fib(纤维蛋白原),探讨其在肺癌患者中的临床意义。方法肺癌组(256例)、肺良性疾病组(200例)和正常对照组(200例)的血清NSE、CYFRA21-1水平采用电化学发光分析法检测,血

  8. The differential diagnosis value of CEA, CYFRA21-1, NSE and LDH in the blood and pleural fluid of patients with benign or malignant pleural effusion%CEA、CYFRA21-1、NSE、LDH在良恶性胸腔积液中的鉴别诊断价值

    Institute of Scientific and Technical Information of China (English)

    巩强进; 吕志; 吴丹; 胡先纬; 胡杰贵

    2012-01-01

    Objective To determine the differential diagnosis value of the tumor markers Carcinoembryonic Antigen( CEA ), Cy-tokeratin 19 fragment (CYFRA21-1 ),Neuron Specific Enolase( NSE ) and LDH in the blood and pleural fluid of patients with benign or malignant pleural effusion. Methods We prospectively evaluated the value of CEA, CYFRA21-1, NSE and LDH in the blood and pleural fluid of 108 patients with pleural effusion. Of which, 55 were diagnosed with malignant pleural effusion, and 53 were diagnosed with benign effusion. The optimum cut-off points resulting from the best sensitivity-specificity balance in the ROC curves were constructed. Results There were significant differences between malignant and benign cases for CEA and CYFRA21-1, in blood,and for CYFRA21-1、NSE、 CEA、LDH in pleural fluid . In the pleural fluid, the sensitivity and specificity of CEA, CYFRA21-1, NSE and LDH was 90. 9、63. 6、 72.7、36.4% and 98. K83、67. 9、88.1% .respectively. In the blood, the sensitivity and specificity was 89. K87. 3、32. K25. 5% and 92. 5、79. 2、90. 6、92. 5% for CEA, CYFRA21-1, NSE and LDH, respectively. Conclusions The results suggest that these markers might be useful in the differentiation between malignant and benign pleural effusion.%目的 探讨血清及胸腔积液中癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经特异性烯醇化酶(NSE)、乳酸脱氢酶(LDH)在良恶性胸腔积液鉴别诊断价值.方法 分析我院55例肺癌患者和53例良性胸腔积液患者的血清及胸腔积液中CEA、CYFRA21-1、NSE、LDH检测水平,并根据受试者工作特性(ROC)曲线建立合理的临床判断临界值及检测的敏感性和特异性.结果 恶性患者胸水CYFRA21-1、NSE、CEA、LDH的水平高于良性患者(P<0.05).胸水CEA、CYFRA21-1、NSE、LDH的敏感性和特异性分别为90.9、63.6、72.7、36.4%和98.1、83、67.9、88.7%.血清CEA、CYFRA21-1、NSE、LDH的敏感性和特异性分别为89.1、87.3 、32.1

  9. Logistic回归和ROC曲线综合评价CEA、NSE和CYFRA21-1对肺癌的诊断价值%Evaluation of the diagnostic values of serum CEA, NSE and CYFRA21-1 in lung cancer

    Institute of Scientific and Technical Information of China (English)

    林卫虹; 廖伟娇; 郑君德; 钟丽红

    2011-01-01

    Objective To explore the application of serum carcinoembryonic antigen (CEA), neuron-specificenolase (NSE) and cytokeratin 19 fragment (CYFRA21-1) for the diagnosis of lung cancer.Methods The levels of serum CEA ,NSE and CYFRA21-1 in patients with lung cancer,patients with benign lungs diseases were determined by electrochemiluminescence (ECLIA).Logistic regression and ROC curve were applied to analyze the data and evaluate the diagnostic values.Results The concentrations of serum CEA, NSE and CYFRA21-1 in patients with lung cancer were significantly higher than the other two groups (P<0.01).The serum level of CEA was highest in patients with adenocarcinoma.NSE was the most sensitive tumor marker (81.6%)for small cell lung cancer.When the specificity was 90% ,CYFRA21-1 had the highest sensitivity in lung cancer.According to the regression model, Y=l/[1 +EXP(5.830-0.249X1-0.198X2-0.643X3)], the sensitivity, specificity and accuracy of the new variable Y was 80.9%, 91.3% and 84.6%, respectively.Conclusion Serum CEA, NSE and CYFRA21-1 in lung cancer has a high diagnostic value.Tbe use of Logistic regression and ROC curve can improve the diagnosis of lung cancer.%目的 应用Logistic回归和ROC曲线探讨血清癌胚抗原(carcinoembryonic antigen,CEA)、神经元特异性烯醇化酶(neuron-specificenolase,NSE)和细胞角蛋白19片段(cytokeratin 19 fragment,CYFRA21-1)在肺癌诊断中的应用.方法 采用电化学发光免疫分析仪(E170)检测不同病理类型肺癌组、肺良性疾病组以及健康人血清CEA、NSE和CYFRA21-1的水平,通过Logistic回归建立回归模型,用ROC曲线分析三指标对肺癌诊断的意义.结果 肺癌组CEA、NSE和CYFRA21-1的水平显著高于肺良性疾病和健康人组(P<0.01).腺癌组血清CEA水平最高,NSE在小细胞肺癌中灵敏度最高(81.6%),取特异性90%时,CYFRA21-1对肺癌的诊断灵敏度最高(59.5%).建立回归模型Y=1/[1+EXP(5.830-0.249X1-0.198X2-0.643X3)],新变量Y

  10. CYFRA21-1、CEA、NSE和CA125联合检测在非小细胞肺癌中的诊断价值%The Diagnostic Value of Combined Detection of CYFRA21-1,CEA,NSE and CA125 in Non Small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    邢国燕; 赵树波

    2016-01-01

    Objective:To discuss the diagnostic value of combined detection of CYFRA21-1,CEA, NSE and CA125 in non small cell lung cancer.Method:92 cases of NSCLC and 95 cases of lung benign lesions from January 2010 to June 2015 in our hospital were selected as the research objects,92 cases of NSCLC were divided into 42 cases of lung squamous cell carcinoma group and 50 cases of lung adenocarcinoma group according to the different pathological types.The levels of CYFRA21-1, CEA, NSE and CA125 in all patients were measured by electrochemical luminescence method.Result:The CYFRA21-1,CEA,NSE and CA125 in NSCLC group were significantly higher than those in benign lung disease group,the differences were statistically significant (P<0.05).The sensitivity and specificity of CYFRA21-1 were respectively 71.40% and 89.10% in lung squamous cell carcinoma group,the sensitivity and specificity of CEA were respectively 60.00% and 84.30% in lung adenocarcinoma group.The sensitivity of CYFRA21-1 in the diagnosis of NSCLC was 46.53%,specificity was 85.26%.The sensitivity of CEA in the diagnosis of NSCLC was 42.58%,specificity was 81.05%.The sensitivity of NSE in the diagnosis of NSCLC was 57.43%,specificity was 76.85%.The sensitivity of CA125 in the diagnosis of NSCLC was 48.51%,specificity was 66.32%.Combined determination of the four parameters sensitivity was 93.26%,specificity was 98.25%.Conclusion:CYFRA21-1,CEA,CA125 and NSE have certain value for the early detection and diagnosis of NSCLC,and the combined detection of the four has obvious complementarity, which can significantly improve the diagnostic rate of NSCLC.%目的:探讨细胞角蛋白19片段(CYFRA21-1)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、糖类抗原125(CA125)联合检测在非小细胞肺癌(NSCLC)中的诊断价值。方法:选取2010年1月-2015年6月本院收治的NSCLC患者92例和肺部良性病变患者95例作为研究对象,92例NSCLC患者根据病理类

  11. 联合检测血清CEA、CYFRA21-1、NSE在肺癌诊断、分期、分型中的意义%Clinical Values of Combined Serum CEA,CYFRA21-1 and NSE Detection in Diagnosis,Staging and Predicting Pathologic Typing of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    徐恩赐; 王利利; 周菊英

    2012-01-01

    目的 探讨血清癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)在肺癌诊断、临床分期以及病理分型中的作用.方法 采用化学发光法、放射免疫分析法和酶联免疫吸附法分别检测114 例肺癌患者和89例肺部良性疾病患者血清CEA、CYFRA21-1、NSE水平.结果 肺癌组血清CEA、CYFRA21-1、NSE含量明显高于肺良性疾病组(P<0.05);CEA、CYFRA21-1及NSE诊断肺癌的ROC曲线下面积分别是0.60、0.85、0.82;联合检测对肺癌诊断的灵敏度明显高于任何单一检测(P<0.05);Ⅰ+Ⅱ期肺癌组血清CEA含量明显高于肺良性疾病组(P<0.05);CEA 在腺癌组含量明显高于鳞癌组(P<0.05);NSE在小细胞肺癌组含量明显高于鳞癌组和腺癌组(P<0.05);血清CEA在Ⅰ、Ⅱ、Ⅲ期非小细胞肺癌组含量明显低于Ⅳ期(P<0.05);CYFRA21-1在Ⅰ、Ⅱ期非小细胞肺癌组含量明显低于Ⅲ、Ⅳ期(P<0.05).结论 CEA对肺癌的早期诊断有一定意义;血清CEA对腺癌的灵敏度最佳,NSE对小细胞肺癌的灵敏度最佳;早期肺癌血清CEA、CYFRA21-1水平明显低于晚期肺癌;三种肿瘤标志物联合检测可明显提高肺癌诊断的敏感性,对肺癌的临床诊断、病理分型、临床分期具有重要的临床意义.%Objective To evaluate the value of combined serum carcinoembryonic antigen(CEA),cytokeratin fragment 19(CYFRA21-1) and neuron-specific enolase(NSE) detection for diagnosis,staging and predicting of pathologic types in patients with pulmonary carcinoma.Methods The levels of CEA,CYFRA21-1 and NSE were measured in 114 patients with pulmonary carcinoma and 89 patients with pulmonary benign lesion,using chemoluminescence,RIA and ELISA methods respectively.Results The concentrations of CEA,CYFRA21-1 and NSE in the cancer patients group were obviously higher than those in benign lung diseases group(P < 0.05). In single index the ROC area of serum CEA,CYFRA21-1 and NSE was 0

  12. Expression and clinical significance of Twist,VEGF,CEA,CYFRA21-1,NSE in malignant pleural effusion of patients with lung cancer%Twist蛋白、VEGF、CEA、CYFRA21-1、NSE在肺癌患者恶性胸水中的表达和应用

    Institute of Scientific and Technical Information of China (English)

    韩娟; 颜浩; 张凌

    2016-01-01

    目的:探讨肺癌患者恶性胸水中Twist蛋白、血管内皮生长因子(VEGF)、癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经原特异性烯醇化酶(NSE)的表达及其临床意义。方法采集52例肺癌患者恶性胸腔积液标本,其中腺癌24例,鳞癌16例,小细胞肺癌12例,良性胸腔积液患者45例作为对照。应用酶联免疫吸附试验法和化学发光法检测各指标水平。结果肺癌组恶性胸水中 Twist 蛋白、VEGF、CEA、CYFRA21-1NSE 的表达均高于良性肺病组(t值分别为8.67、9.11、3.94、5.37、3.10,P值均0.05),CEA 水平在腺癌中最高,CYFRA21-1水平在鳞癌中最高,NSE水平在小细胞肺癌中最高。Twist 蛋白、VEGF、CEA、CYFRA21-1NSE 在肺癌恶性胸水中的敏感度分别为78.8%、84.6%、61.5%、46.2%、42.3%,特异度分别为86.7%、88.9%、91.1%、86.7%、77.8%。CEA、CYFRA21-1、NSE在不同肺癌恶性胸水中敏感度不同,联合检测可提高敏感度。结论胸水中 Twist 蛋白、VEGF、CEA、CYFRA21-1NSE 检测有利于良、恶性胸水的鉴别, Twist蛋白、VEGF水平与肿瘤病理类型无关,CEA、CYFRA21-1NSE 水平与肿瘤病理类型相关,联合检测可提高诊断的敏感度。%Objective To investigate the expression and clinical significance of Twist protein, vascular endothelial growth factor (VEGF),carcino-embryonic antigen (CEA),cytokerantin-1 9-fragment (CYFRA2 1-1),neuron-specific enolase (NSE) in lung cancer patients with malignant pleural effusion. Methods The specimens of 52 cases of lung cancer with malignant pleural effusion were gathered, including 24 cases of adenocarcinoma,16 case of squamous cell carcinoma,and 12 cases of small cell lung cancer.45 patients with benign lung diseases were selected as controls.The expressions of markers were detected by enzyme-linked immunosorbent assay and chemical luminescence method.Results The expressions of Twist protein,VEGF,CEA,CYFRA2 1-1 ,NSE in malignant pleural

  13. 99Tcm-HL91乏氧显像联合血清CYFRA21-1、NSE、CEA检测对肺癌的诊断价值%The Clinical Value of 99Tcm-HL91 Hypoxia Imaging Combined with the Detection of Serum NSE, CEA and CYFRA21-1 in the Diagnosis of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    李晓红; 王迎秋; 孙高峰; 张桉瑜; 左长京

    2012-01-01

    Objective To investigate the value of 99Tcm-HL91 hypoxia imaging combined the detection of serum neuron-specific enolase (NSE) , careinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1) in the diagnosis of lung cancer. Methods 63 patients with lung neoplasm were carried out 99Tcm-HL91 hypoxia imaging, and the serum tumor markers levels of NSE, CEA and CYFRA21-1 were detected. The sensitivity, specificity and accuracy of each and combined method were calculated respectively and compared with each other. Results The sensitivity, specificity and accuracy in diagnosis of lung cancer with 99Tcm-HL91 hypoxia imaging were 85. 4%, 100% and 88. 9% respectively; and with serum tumor markers NSE, CEA and CYFRA21-1 detection were 72.9% , 86.7% and 76.1% respectively. Compared with the detection of serum tumor marker levels of NSE, CEA and CYFRA21-1, 99Tcm-HL91 hypoxia imaging had the higher sensitivity and specificity, and similar accuracy. The sensitivity, specificity and accuracy of combined two methods in diagnosis of lung cancer were 95. 8% , 86.7% and 93.6% respectively. The sensitivity and accuracy of the combined method were significantly increased than single method ( P 0.05). Conclusion 99Tcm-HL91 hypoxia imaging and the detection of serum level of NSE, CEA and CYFRA21-1 have an important diagnostic value, and their combination could improve the sensitivity and accuracy in the diagnosis of lung cancer.%目的 探讨99Tcm-HL91乏氧显像和血清细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)、癌胚抗原(CEA)检测及其联合应用诊断肺癌的价值.方法 选择肺部肿瘤患者63例,全部行99Tcm-HL91乏氧显像和血清CYFRA21-1、NSE、CEA三种肿瘤标志物检测,分别计算两种方法及两种方法联合诊断肺癌的灵敏度、特异性和准确率并加以比较.结果 99Tcm-HL91乏氧显像诊断肺癌的灵敏度为85.4%、特异性为100%、准确率为88.9%,CYFRA21-1、NSE、CEA联合

  14. 肺癌临床分期与肿瘤标志物CEA、CA15-3、NSE和CYFRA21-1相关性分析%Correlation analysis of lung cancer staging and tumor markers CEA, CA15-3, NSE and CYFRA21-1

    Institute of Scientific and Technical Information of China (English)

    聂书伟; 吕勇刚; 易军

    2012-01-01

    Objective To study the correlation between serum tumor marker carcinoembryonic antigen (CEA) , carbohydrate antigen 15-3 (CA15-3) , neuron-specific enzyme diluted alcohol (NSE) , cytokeratin 19 fragment (CYFRA21-1) and clinical staging of lung cancer, and discuss its clinical significance. Methods One hundred and sixty-Bix cases of lung cancer was diagnosed by pathology or cytology, including 81 cases of adenocarcinoma, 44 cases of squamous cell carcinoma, 14 cases of small cell carcinoma, 12 cases of adenosquamous carcinoma, 11 cases of combined small cell carcinoma and 4 cases of other; non metastasis in 109 cases and metastasis in 57 cases. 36 cases of health people in health checks in the hospital at same period were as normal controls. Serum CEA, CA15-3, NSE and CYFRA21-1 levels were measured by -y radioimmunoassay in patients with lung cancer and normal controls; and compared with lung cancers staging, metastasis and without metastasis in the expression levels and positive rate of tumor markers, and the correlations were analyzed. Results There was lung cancer of clinical stage, including I stage 10. 24% (17/166), II stage 7.23% (12/166), III stage 36.75% (61/166) and IV stage 45.78% (76/166). With the staging of lung cancer increased serum levels of tumor markers (CEA, CA15-3 and CYFRA21-1) were rising trend (P<0. 05) , the later stages of lung cancer, the higher the positive rate of tumor markers (P < 0. 05). Lung cancer metastasis was diagnosed in 57 (27.67%) patients, tumor marker levels after the metastasis were significantly higher than those without metastasis (P < 0.01). The control group and lung cancer patients were observed as the object, the combination tests of CEA, CAl 5 -3, NSE and CYFRA21 -1 levels werebetter than single test, and sensitivity (71. 93 % ) increased, false negative (28.07%) was lower, but specificity (97.25%) and false positive (2. 75% ) had no significant change. Conclusion The detection of CEA, CA15-3, NSE and CYFRA21-1

  15. CYFRA 21-1 as a tumor marker for squamous cell carcinoma of the esophagus.

    Science.gov (United States)

    Nakamura, T; Ide, H; Eguchi, R; Hayashi, K; Takasaki, K; Watanabe, S

    1998-01-01

    This study assessed the clinical value of CYFRA 21-1 in comparison with squamous cell carcinoma antigen (SCC-Ag), carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) in patients with esophageal squamous cell carcinoma. In 112 primary cancer patients, the diagnostic sensitivity of CYFRA 21-1 (33.9%) was superior to SCC-Ag (28.6%), CEA (12.5%), and CA19-9 (6.3%). Levels of CYFRA 21-1 were closely correlated with TNM stage and wee below the cutoff value in all 21 patients with stage I disease. All 38 patients with a CYFRA 21-1 level over the cutoff value among the 80 patients who underwent esophagectomy had lymph node metastases (pN1). A correlation was found between CYFRA 21-1 levels and clinical response in serial measurements of 21 patients who received chemotherapy or chemo radiotherapy. Our findings suggest that CYFRA 21-1 is not useful for diagnosis, but that it is valuable for monitoring the efficacy of therapy.

  16. Value of combined detection of serum SCC, CEA, NSE and CYFRA21-1 in the diagnosis of esophageal cancer%血清鳞状上皮细胞癌相关抗原、癌胚抗原、神经元特异性烯醇化酶及细胞角蛋白19片段抗原检测在食管癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    曹红; 江华

    2013-01-01

    Objective To explore the diagnostic value of serum tumor marker SCC, CEA, NSE and CYFRA21-1 detection in early diagnosis of esophageal cancer, and to provide theoretical reference for diagnosis and treatment of esophageal cancer.Methods Serum levels of SCC, CEA, NSE, CYFRA21-1 were detected by full automatic chemilu-minescence immune assay in 86 patients with esophageal cancer (the esophageal cancer group) and 100 healthy controls (the control group).Results ①Serum levels of SCC, CEA, SCE, CYFRA21-1 in esophageal patients were significantly higher than those of in control group (P < 0.05), and when the clinical stage and depth of tumor invasion increased, the level of serum tumor marker was significantly increased (P < 0.05 or P < 0.01).②The positive detection rate of SCC, CEA, NSE and CYFRA21-1 in esophageal cancer group was 26.7%, 36.0%, 24.4%, 53.5%.The four kinds of tumor markers joint detection in early diagnosis of esophageal cancer had higher sensitivity and specificity the sensitivity and specificity were 82.6% and 83.7%.Conclusion Measurement of serum SCC, CEA, SCE and Cyfra21 -1 is helpful in the diagnosis of esophageal, and multiple tumor markers joint detection can improve the sensitivity of clinical diagnosis.%目的 探讨血清肿瘤标志物鳞状上皮细胞癌相关抗原(SCC)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)及细胞角蛋白19片段抗原(CYFRA21-1)在食管癌早期诊断中的应用价值,旨在为食管癌的诊治提供理论参考.方法 应用全自动化学免疫分析检测86例食管癌患者(食管癌组)和100例健康体检者(对照组)血清SCC、CEA、NSE、CYFRA21-1的表达.结果 ①SCC、CEA、NSE、CYFRA21-1在食管癌患者中的含量均明显高于健康人(P < 0.05),并随着临床分期、肿瘤浸润深度的增加而显著增加(P < 0.05或P < 0.01).②食管癌组SCC、CEA、NSE、CYFRA21-1阳性检测率分别为26.7%、36.0%、24.4%、53.5%,敏感性较低;多项血清肿瘤标

  17. Analysis on the value of combined detection of CYFRA21-1,NSE,CEA,CA125 in lung carcinoma%CYFRA21-1、NSE、CEA 和CA125联合检测对肺癌诊断的价值分析

    Institute of Scientific and Technical Information of China (English)

    陈乐

    2016-01-01

    Objective To investigate the value of combined detection by using tumor markers cytokeratin‐19 fragment antigen (CYFRA21‐l) ,neuron‐specific enolase (NSE) ,carcinoembryonic antigen (CEA) and carbohydrate cancer antigen 125(CA125) in lung cancer diagnosis .Methods 200 people who treated in the hospital in related departments from January 2012 to January 2014 were enrolled in the study .100 of them suffered from lung cancer ,53 suffered from benign lung disease ,the rest 47 were healthy people .Electrochemiluminescence method or enzyme‐linked immunosorbent assay(ELISA) were used to detect serum tumor mark‐ers CYFRA21‐1 ,NSE ,CEA ,CA125 and the sensitivity ,specificity and accuracy of different combinations of test items were evalua‐ted .Results In the single detection of those tumor markers for the diagnosis of lung cancer ,CYFRA21‐1 detection got the highest sensitivity and accuracy ,while CA125 got the highest specificity(95 .0% ) .The sensitivity and specificity of combined detection were both significantly higher than single detections .The sensitivity ,pecificity and accuracy of the combined detection of the 4 tumor markers were 92 .0% ,72 .0% ,and 87 .0% respectively .Conclusion In the clinical diagnosis of lung cancer ,the combined detection of several serum tumor markers get higher sensitivity and accuracy than single detection ,which is worthy of clinical promotion .%目的:探讨细胞角蛋白‐19片段抗原(CYFRA21‐1)、神经元特异性烯醇化酶(NSE)、癌胚抗原(CEA )和糖类癌抗原125(CA125)联合检测对肺癌患者的诊断价值。方法从2012年1月至2014年1月该院相关科室共选取研究对象200例,其中肺癌患者100例、肺良性病变患者53例、健康体检者47例。采用电化学发光方法或酶联免疫吸附测定(ELISA )检测患者血清CYFRA21‐1、NSE、CEA、CA125的水平,评价不同检测项目组合在肺癌诊断中的灵敏度、

  18. 99mTc-MDP 全身骨扫描和血清 CEA、NSE、CYFR21-1、CA125 测定对小细胞肺癌骨转移的临床评价%Clinical evaluation of 99mTc-MDP bone scintigraphy and serum CEA, NSE,CYFRA21-1 and CA125 in skeletal metastasis of the small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    张炜; 郭万华

    2015-01-01

    目的:分析99m Tc-MDP全身骨扫描小细胞肺癌骨转移病灶分布特点及血清肿瘤标志物CEA、NSE、CYFRA21-1、CA125与小细胞肺癌骨转移发生的相关性. 方法:回顾性分析56例经病理证实的小细胞肺癌患者 99m Tc-MDP全身骨扫描影像表现及血清肿瘤标志物水平,用ROC曲线及Spearman相关分析评价肿瘤标志物水平对肺癌骨转移发生及发展的诊断意义. 结果:21 例骨转移阳性病灶中胸部占46.60%,脊柱34.95%,骨盆9.71%,四肢5.83%,头颅2.91%. 骨转移阳性组CEA、NSE 质量浓度高于骨转移阴性组(均P<0.05),曲线下面积(AUC)分别为0.789、0.717,且CEA、NSE质量浓度与骨转移分级呈正相关(r值分别为0.540、0.417). 结论:小细胞肺癌以胸部及脊柱等中轴骨多发性骨转移为主,CEA、NSE血清水平随着骨转移病灶数的增加有显著上升趋势,对判定小细胞肺癌骨转移的发生及发展有一定的参考意义.%Objective:To analyze the distribution characteristic of the skeletal metastasis lesion in 99mTc-MDP bone scintigraphy and the correlation between serum tumor marker (CEA,NSE, CYFRA21-1 and CA125) and skeletal metastasis in small cell lung cancer .Methods:56 cases with small cell lung confirmed by pathology were examined with 99m Tc-MDP bone scintigraphy .Serum concentration of CEA , NSE, CYFRA21-1 and CA125 were measured one week before whole-body bone scanning .ROC curve and spearman correlation analysis were used to evaluate the serum tumor markers in diagnose the development of the skeletal metastasis .Results: 21 imaging positive lesions were identified as the skeletal metastasis .The distribution of the skeletal metastasis lesions were:chest(46.60%), spine(34.95%), pelvis(9.71%), four limbs(5.83%), skull(2.91%).CEA,NSE levels in bone metastasis group were significant higher than those in the negative group ( P<0.05 ) and the area of ROC curve were 0.789 and 0.717 respectively(P<0.05).There was a correlation between

  19. Serum CYFRA 21-1 in Egyptian women with breast cancer | Ebied ...

    African Journals Online (AJOL)

    Serum CYFRA 21-1 in Egyptian women with breast cancer. ... in the diagnosis and management of lung cancer, particularly of squamous cell histotype. ... clinicopathological factors and prognosis in comparison with serum CA15.3 and CEA ...

  20. Circulating CYFRA 21-1 is a Specific Diagnostic and Prognostic Biomarker in Biliary Tract Cancer

    Science.gov (United States)

    Chapman, Michael H; Sandanayake, Neomal S; Andreola, Fausto; Dhar, Dipok K; Webster, George J; Dooley, James S; Pereira, Stephen P

    2011-01-01

    Background Biliary tract cancer (BTC) has a poor prognosis, in part related to difficulties in diagnosis. Cytokeratin 19 (CK19) is a constituent of the intermediate filament proteins of epithelial cells. CK19 fragments (CYFRA 21-1) are rarely identified in the blood of healthy individuals. We assessed the utility of CYFRA 21-1 as a diagnostic and prognostic marker of BTC. Methods Blood was prospectively collected from patients with benign biliary disease (n = 39), primary sclerosing cholangitis (n = 19), PSC-related cholangiocarcinoma (n = 6) and sporadic BTC (n = 60). CYFRA 21-1 levels were measured in duplicate by ELISA. Results CYFRA 21-1 (≤1.5 ng/mL) had a sensitivity of 56% and specificity of 88%, compared with figures of 79% and 78% for CA 19-9 (≤37 U/mL). Using a higher cut-off of 3 ng/mL, CYFRA 21-1 had a sensitivity of 30% and specificity of 97%. Combination of CYFRA 21-1 (≤1.5 ng/mL) and CA 19-9 (≤37 U/mL) resulted in sensitivity and specificity of 45% and 96%. In contrast to CA 19-9, CYFRA 21-1 (≤3.0 ng/mL) alone was a strong predictor of prognosis (median survival 2 months vs 10 months, p = 0.001). Conclusion Elevated circulating CYFRA 21-1 is a specific, but less sensitive diagnostic marker than CA 19-9, predicts a poor outcome and may act as a surrogate marker of circulating tumor cells in BTC. Further prospective studies of its utility in assessing operability and response to chemotherapy are needed. PMID:22228935

  1. 人血清CYFRA21-1化学发光免疫分析方法的初步建立及应用%The Preliminary Establishment and Application of Chemiluminescence Immunoassay for Detection of Human CYFRA21 -1 in Serum

    Institute of Scientific and Technical Information of China (English)

    潘慧杰; 刘红; 唐磊; 李嘉; 杨萌萌; 杨铁生; 樊春红; 杨晓林; 孙旭东

    2012-01-01

    Objective To estimate an enhanced chemiluminescence immunoassay for detection CYFRA21-1 in serum.Methods CYFRA21-1 chemiluminescence quantitative immunoassay kit was used.308 clinical samples were detected, compared with ECLA CY-FRA21-1 immunoassay.Results The sensitivity of the assay was 0.04μg/L.The linear range was (1.5 ~ ICO) μg/L, and there was no significant cross-reaction with NSE or CEA.The recovery rate of addition and dilution were both below 10% .Besides, the intra- and inter-coefficients of variations were from 90% to 110% .The 0.95 confidence interval for normal samples was 0 ~3.4μg/L (n =231) .Comparing to ECLA, the clinical accordance rate was 96.65% .Conclusion The sensitivity, specificity, stability and detection range of this method are all good enough to meet the clinical requirement, furthermore, to take the place of the foreign like products.%目的:建立人血清CYFRA21-1化学发光免疫分析方法并在临床检测中的应用.方法:使用CYFRA21-1定量测定试剂盒(化学发光法)检测308例临床血清标本,并与CYFRA21-1电化学发光全自动免疫分析方法进行对比.结果:灵敏度0.04μg/L,线性范围(1.5~100)μg/L,与NSE及CEA无交叉反应,样本中抗凝剂量的柠檬酸钠、肝素、EDTA-Na2对测定结果没有影响.添加回收率和稀释回收率为90%~110%,分析内和分析间变异系数均<10.0%.该方法正常参考值为(0~3.4)μg/L(95%可信限).ECLA测定结果与本法相比,临床总符合率为96.65%.结论:该方法灵敏度高、特异性好、稳定性强,检测范围宽,有良好的准确性和重复性,完全可替代进口化学发光试剂用于临床样本的检测.

  2. Analysis in patients of the Hospital A.C. Camargo of two tumors markers: CA-72.4 and CYFRA-21.1; Analise em pacientes do Hospital A.C. Camargo de dois marcadores tumorais: CA-72.4 e CYFRA-21.1

    Energy Technology Data Exchange (ETDEWEB)

    Barbuto, Jose Alexandre Marzagao; Oda, Emilia Emiko; Oliveira, Ricardo Manoel de [Fundacao Antonio Prudente, Sao Paulo, SP (Brazil). Hospital A.C. Camargo

    1995-03-01

    The behavior of two tumor markers, CA-72.4 and CYFRA-19.9 was analysed in patients of the Hospital A.C. Camargo. The measurement of CA-272.4 serum levels was performed by an automated chemo luminescence assay in 49 patients and proved useful in the two groups analyzed, patients with esophageal tumors and with gastric tumors. In both cases, there was an increase in sensitivity when measurements of this marker was added to that of carcinoembryonic antigen (CEA) and of CA-19.9. The most sensitive associations were of CA-72.4 and CA-19.9 for esophageal tumors and of CA-72.4 and CEA for the gastric tumors. The maker CYFRA-21.1 was analyzed by a radioimmunoassay in 102 normal blood donors and in 20 patients with lung cancer. Among these, CYFRA-21.1 was altered in 55% of the cases. Therefore, CYFRA-21.1 could be considered, as the neuron-specific enolase (NSE), very useful tool for the follow-up lung cancer patients. (author) 6 refs., 3 figs., 3 tabs.

  3. [A case of advanced gastric cancer showing high serum CYFRA21-1 level responding to chemotherapy with S-1].

    Science.gov (United States)

    Hara, Akihito; Watanabe, Hiroaki

    2008-12-01

    The patient was a 75-year-old man whose complaint was back pain and appetite loss. He was diagnosed with unresectable advanced gastric cancer due to multiple liver metastases and direct invasion of pancreas and spleen. He underwent gastrostomy because of esophageal stenosis, and we administered S-1 80 mg/body(4 weeks administration and 2 weeks rest)to the patient through a gastrostogavage tube. On blood examination, the serum level of CYFRA21- 1 was significantly high, while those of CEA and CA19-9 were within normal ranges. After the first course of this chemotherapy, the serum CYFRA21-1 level significantly decreased with reduction of the cancer. After the second course, it sensitively increased before image views detected the progression of the cancer. This case shows that CYFRA21- 1 could be a useful tumor marker of advanced gastric cancer.

  4. Prognostic value of serum CYFRA21-1 and CEA for non-small-cell lung cancer.

    Science.gov (United States)

    Zhang, Zhi-Hui; Han, Yun-Wei; Liang, Hui; Wang, Le-Min

    2015-11-01

    The aim of the study was to assess the clinical prognostic value of serum cytokeratin 19 fragment (CYFRA21-1) and carcinoembryonic antigen (CEA) for non-small-cell lung cancer (NSCLC) patients. Literatures related to effects of serum CYFRA21-1 and CEA on the prognosis of lung cancer patients were retrieved from databases such as PubMed, Springer Link, Embase, Wanfang, and CNKI. Meta-analysis was carried out using RevMan 5.1 software. Ten literatures involving 1990 NSCLC patients were selected in this study. Total survive estimation merging hazard ratio (HR) in all NSCLC patients with high-level serum CYFRA21-1 was 1.64 (95% CI 1.46-1.84, P < 0.001) and that in all NSCLC patients with high level serum CEA was 1.46 (95% CI 1.28-1.65, P < 0.001). Serum CYFRA21-1 and CEA can be used as prognostic factors of NSCLC patients. Combinative detection of the two indices will be more reliable.

  5. Assessment of Confounding Factors Affecting the Tumor Markers SMRP, CA125, and CYFRA21-1 in Serum

    Directory of Open Access Journals (Sweden)

    Daniel Gilbert Weber

    2010-01-01

    Full Text Available The purpose of this analysis was to evaluate if serum levels of potential tumor markers for the diagnosis of malignant mesothelioma and lung cancer are affected by confounding factors in a surveillance cohort of workers formerly exposed to asbestos. SMRP, CA125, and CYFRA21-1 concentrations were determined in about 1,700 serum samples from 627 workers formerly exposed to asbestos. The impact of factors that could modify the concentrations of the tumor markers was examined with linear mixed models. SMRP values increased with age 1.02-fold (95% CI 1.01–1.03 and serum creatinine concentration 1.32-fold (95% CI 1.20–1.45. Levels differed by study centers and were higher after 40 years of asbestos exposure. CA125 levels increased with longer storage of the samples. CYFRA21-1 values correlated with age 1.02-fold (95% CI 1.01–1.02, serum creatinine 1.21-fold (95% CI 1.14–1.30 and varied by study centers due to differences in sample handling. Tumor marker concentrations are influenced by subject-related factors, sample handling, and storage. These factors need to be taken into account in screening routine.

  6. 慈菇对CCl4致大鼠肝纤维化血清cyfra21-1和CEA影响的探讨%The Effect of Sagittaria Agittifolia on Changes of Cyfra21-1 and CEA in Rats of CCl4 Experimental Liver Fibrosis

    Institute of Scientific and Technical Information of China (English)

    吴小南; 汪家梨; 盛健; 黄建宏

    2001-01-01

    目的探讨慈菇对CCl4致大鼠肝纤维化的干预作用.方法对5月龄Wistar大鼠,用CCl4染毒致大鼠形成肝组织纤维化,以ELISA法和放射免疫分析法检测大鼠血清cyfra21-1和CEA的浓度的变化,并观察慈菇的干预效果.结果染毒组大鼠cyfra21-1和CEA明显高于对照组,慈菇可减轻CCl4致大鼠肝纤维化程度并减少cyfra21-1和CEA的产生.结论大鼠肝纤维化后血清cyfra21-1和CEA升高,提示慈菇干预作用的机理值得深入探讨.

  7. Prognosis evaluation value of serum CYFRA21-1 and CEA level in non-small cell lung cancer:Meta analysis%血清CYFRA21-1和CEA对非小细胞肺癌预后评价价值的Meta分析

    Institute of Scientific and Technical Information of China (English)

    邓伟伟; 黄艳春

    2014-01-01

    Objective To evaluate the clinical significance of serum cytokeratin 19 fragment (CYFRA21-1) and carcinoma embryonic antigen(CEA) prognosis in non-small cell lung cancer(NSCLC) patients.MethodsAll the data dealing with the significance of serum CYFRA21-1 and serum CEA prognosis of NSCLC was researched from PubMed, Springer Link and Du Xiu database.The outcome was measured using the hazard ratio(HR). Data pooling was performed by RevMan 5.1.Results One thousand five hundred and sixty-nine patients from 8 studies were included in the meta analysis. Merger HR estimate of overall survival for NSCLC patients with high serum CYFRA21-1 level was 1.63 (95%CI: 1.43-1.85,P<0.05). Merger HR estimate of overall survival for NSCLC patients with high serum CEA level was 1.45 (95%CI: 1.26-1.66,P<0.05).ConclusionSerumCYFRA21-1 and CEA can be regarded as a prognostic indicator of evaluation of NSCLC, high serum CYFRA21-1 in evaluation of NSCLC with poor prognosis is more valuable than high serum CEA, jointing detection will be more reliable.%目的:研究血清细胞角蛋白19片段(CYFRA21-1)和癌胚抗原(CEA)对非小细胞肺癌(NSCLC)患者的临床预后意义。方法通过检索PubMed、Springer Link、超星读秀中有关研究血清CYFRA21-1和CEA对NSCLC患者预后影响的文献,采用RevMan5.1软件进行荟萃分析(Meta分析),合并值为风险比(HR)。结果共入选8篇文献,累计NSCLC患者1569例。所有高水平血清CYFRA21-1的NSCLC患者的总生存估计合并HR=1.63[95%可信区间(95%CI):1.43~1.85, P<0.05],所有高水平血清CEA的NSCLC患者的总生存估计合并HR=1.45(95%CI:1.26~1.66, P<0.05),差异均有统计学意义。结论血清CYFRA21-1和CEA可作为NSCLC患者的预后评价指标,高血清CYFRA21-1较高血清CEA对评价NSCLC患者的预后不良更有价值,联合检测将更可靠。

  8. The diagnostic value of the levels of CYFRA21-1,Pentraxin-3,ProGRP for non-small cell lung cancer%CYFRA21-1、Pentraxin-3、ProGRP对非小细胞肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    芦珂

    2016-01-01

    Objective To investigate the value of the levels of cytokeratin fragment 19 (CYFRA21-1),serum pen-traxin-3 (Pentraxin-3),gastrin releasing peptide precursor (ProGRP)in non-small cell lung cancer.Methods The lev-els of Pentraxin-3,CYFRA21-1,ProGRP of 62 cases of NSCLC patients,62 cases of benign lung disease group and 60 healthy control subjects were determined with ELISA from October 2013 to October 2014.The value of Pentraxin-3, CYFRA21-1,ProGRP of NSCLC patients with Subjects specific curve (ROC).Results The levels of Pentraxin-3,CY-FRA21-1,ProGRP of NSCLC groups were higher than those with benign lung disease group and the healthy control group (P <0.05).The levels of Pentraxin-3,CYFRA21-1,ProGRP ofpartial response (PR),complete remission (CR) were higer than stable disease (SD),partial response (PR)(P <0.05).The sensitivity and specificity of entraxin-3,CY-FRA21-1,ProGRP single diagnosis were higher than joint diagnostic (P<0.05).Conclusion Serum Pentraxin-3,CY-FRA21-1,ProGRP level has a certain value in the early diagnosis and prognosis of NSCLC,the combination of the three can improve the diagnostic accuracy of diagnosis can be used as an early NSCLC diagnosis and prognosis markers.%目的:探讨细胞角蛋白片段19(CYFRA21-1)、血清穿透素-3(Pentraxin-3)、胃泌素释放肽前体(ProGRP)在非小细胞肺癌(NSCLC)中的应用价值。方法2013年10月至2014年10月选取62例 NSCLC 患者、62例肺部良性疾病组及60例健康对照者,采用 ELISA 法测定三组血清 Pentraxin-3、CYFRA21-1、ProGRP 水平,采用受试者特异曲线(ROC)评价血清 Pentraxin-3、CYFRA21-1、ProGRP 在早期 NSCLC 患者中的应用价值。结果NSCLC 组血清Pentraxin-3、CYFRA21-1、ProGRP 水平显著高于肺部良性疾病组、健康对照组,差异有统计学意义(P <0.05)。NSCLC 组患者中疾病进展(PD)患者血清 Pentraxin-3、CYFRA21-1、ProGRP 水平显著高于疾病稳定(SD)、部分缓

  9. 肾盂尿路上皮癌患者尿 NMP22、TPS、CYFRA21-1、CA19-9的检测与评估%Evaluation of NMP22,TPS,CYFRA21-1 and CA19-9 in the urine of patients for the diagnosis of urothelial carcinoma of renal pelvis

    Institute of Scientific and Technical Information of China (English)

    李志斌; 陈惠庆; 米振国; 宋继文; 韩雪冰; 韩存芝

    2016-01-01

    目的探讨尿液核基质蛋白22(nuclear matrix protein 22,NMP22)、组织多肽特异性抗原(tissue polypeptide-specific antigen,TPS)、CYFRA21-1、糖链蛋白19-9(carbohydrate antigen 19-9,CA19-9)对肾盂尿路上皮癌的诊断价值.方法回顾性分析山西省肿瘤医院2010年1月至2015年12月检测的至少两种上述尿液肿瘤标志物的患者资料,共218人次,包括肾盂尿路上皮癌63例(A 组)、膀胱尿路上皮癌46例(B 组)、非尿路上皮肿瘤的泌尿系其他疾病62例(C 组)、A组行根治术后2年未复发47例(D 组),另有健康志愿者20例(E 组).比较各组尿 NMP22、TPS、CYFRA21-1、CA19-9表达水平,并绘制受试者工作特征(receiver operating characteristic,ROC)曲线,计算 ROC 曲线下面积.结果 A 组 NMP22水平高于 B 组,差异有统计学意义(P =0.001). A 组与 B 组的 TPS、CYFRA21-1、CA19-9水平差异无统计学意义(P >0.05).A 组和 B 组的 TPS、NMP22、CYFRA21-1、CA19-9水平均高于 C 组、D 组、E 组,差异有统计学意义(P <0•05).C 组、D组及 E 组的 NMP22、TPS、CYFRA21-1、CA19-9水平差异无统计学意义(P >0.05). A 组中NMP22、TPS、CYFRA21-1、CA19-9水平在不同病理分级、临床分期、肿瘤大小、是否肾积水等的差异无统计学意义(P >0.05).A 组 NMP22、TPS、CYFRA21-1、CA19-9的诊断准确率高于尿脱落细胞学检查,差异有统计学意义(P <0.05).四种肿瘤标志物的 ROC 曲线下面积为0.7~0.9,诊断效能中等,最高者为 NMP22与 CYFRA21-1的组合,为0.884.结论尿液 NMP22、TPS、CYFRA21-1、CA19-9对肾盂尿路上皮癌的诊断有帮助,诊断效能中等,但它们对肾盂癌肿瘤分期和病理分级的预测、术后监测、预后判断等方面的作用有待进一步研究.%Objective To study the diagonsis value of urinary tissue polypeptide-specific anti-gen (TPS),nuclear matrix protein 22 (NMP22 ),CYFRA21-1 and carbohydrate antigen 1 9-9 (CA1 9-9)in

  10. 影像学征象结合血清PCT、CYFRA21-1对孤立性肺结节的评估%Evaluation of Solitary Pulmonary Nodule with Imaging Features and Serum PCT and CYFRA21-1

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    Objective To explore the correlation between dual-source computed tomography (DSCT) features of solitary pulmonary nodule (SPN) and the expression of serum Procalcitonin (PCT) and CYFRA21-1, and the benign and malignancy decision as well. Meth-ods A retrospective analysis was made to the clinical data of 30 patients with SPN surgically treated in our hospital from Jan., 2009 to Dec., 2011; all the patients received DSCT examination and serum PCT and CYFRA21-1 detection and the results were studied for the correlation analysis and SPN benign and malignancy evaluation. Results Chest DSCT showed out a high specificity, sensitivity and ac-curacy for clinical diagnosis of the benign lesions: hamartoma and pulmonary tuberculoma, and alveolar cell carcinoma as well (P0.05); PCT showed out a high specificity, sensitivity and accuracy for clinical diagnosis of pulmonary infection (P0.05);而PCT对肺部感染的诊断临床特异性、敏感性、准确度均较高(P <0.05);CYFRA21-1血清表达对恶性病变诊断的临床特异性、敏感性、准确度均较高(P <0.05)。结论 PCT 及 CYFRA21-1血清标本检测对胸部 DSCT 难以鉴别的肺部感染和恶性肿瘤具有一定的帮助,为SPN的诊断提供了新的依据。

  11. The Diagnostic and Prognostic Value of Tumor Markers (CEA, SCC, CYFRA 21-1, TPS) in Head and Neck Cancer Patients.

    Science.gov (United States)

    Barak, Vivian; Meirovitz, Amichay; Leibovici, Vera; Rachmut, Jacob; Peretz, Tamar; Eliashar, Ron; Gross, Menachem

    2015-10-01

    Establishing prognostic factors is very important in the management of cancer patients. Our aim was to evaluate the clinical significance of a panel of tumor markers, including CEA (Carcino Embryonic Antigen), SCC (Squamous Cell Carcinoma Antigen), TPS (Tissue Polypeptide Specific Antigen) and CYFRA 21-1 in head and neck cancer patients, for assessing treatment response and prognosis of patients. We evaluated 312 blood samples from 143 head and neck cancer patients, from several sub-groups: 82 Larynx Carcinoma pre- and 38 post-therapy, 46 Oral Cavity pre and 29 post-therapy, 12 nasopharynx, 16 parotid and other salivary gland patients. Blood tumor markers levels were evaluated by conventional ELISA assays. Correlations of marker levels to stage of disease, lymph node involvement and therapy, were performed. Serum levels of all four tumor markers were higher before therapy and decreased thereafter in all patients. The decrease in TPS level following therapy was significant (p=0.03). Significantly higher levels of TPS and similarly higher levels of the other tumor markers were demonstrated in advanced disease (stages III and IV) patients, as opposed to early disease (stages I and II) patients (p=0.012). Node positive patients had significantly higher TPS levels as compared to node negative (p=0.02). The same trend was shown by the other markers as well, but did not reach statistical significance. TPS was best correlated to survival of patients; those having low levels had the best clinical outcome and longer survival. CEA, SCC, TPS and CYFRA 21-1 can all serve as useful tumor markers in HNC patients. They assessed response to therapy and were prognostic for recurrence. TPS proved to be the most sensitive predictor of advanced disease and poor prognosis. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  12. 肺癌患者血清中CK19-2G2和Cyfra21-1的测定及其对肺癌的诊断价值%Detection of serum CK19-2G2 and Cyfra21-1 protein and comparison of their diagnostic value for lung cancer

    Institute of Scientific and Technical Information of China (English)

    高佳; 王海; 齐军

    2008-01-01

    Objective To evaluate and compare the value of serum CK19-2G2 and Cyfra21-1 in diagnosis of lung cancer. Methods The serum concentration of CK19-2G2 and Cyfra21-1 was detected in 104 patients with lung cancer, 71 with benign lung diseases and 105 healthy volunteers. The value of the two parameters in the diagnosis of lung cancer was evaluated with ROC curve analysis. The mean level of CK19-2G2 in the three groups was compared with that of Cyfra21-1 by Mann-Whitney U test, and the correlation between these two parameters was calculated using Pearson coefficient correlation test. Results The mean serum CK19-2G2 concentration in the lung cancer group was 2.87 mU/ml, significantly higher than that in the group with benign lung diseases ( 1.02 mU/ml) and healthy volunteer group ( 0. 01 mU/ml) ( P = 0.000). The serum concentration of CK19-2G2 was positively correlated with that of Cyfra21-1, with a Pearson coefficient correlation of 0. 543. Furthermore, the mean serum CK19-2G2 level in squamous cell carcinoma group (8.35 mU/ml) was significantly higher than that in the other pathologic groups (P < 0.05). Conclusion CK19-2G2, with a higher specificity, may be a better tumor marker than CK19-2G2 in respect of specificity for non-small cell lung cancer (NSCLC), especially for squamous lung carcinoma, and deserves further study.%目的 测定肺癌患者血清中细胞角蛋白19(CK19)降解片段CK19-2G2的浓度,并评价其对肺癌的诊断价值.方法 采用CK19-2G2和Cyfr21-1的检测试剂盒,分别检测104例肺癌患者、7l例肺部炎症患者和105例健康体检者的血清标本,采用Pearson相关分析判断二者的相关性.运用受试者工作特性曲线(ROC)分析CKl9-2G2和Cyfra21-1的检测效能,并确定CK19-2G2诊断肺癌的临界值.比较CK19-2G2和Cyfra21-1诊断肺癌的灵敏度和准确率.结果 肺癌组、肺部炎症组和健康对照组血清CK19-2G2的中位浓度分别为2.87、1.02和0.01 mU/ml,肺癌组显著高于肺部炎

  13. Effect of endostar combined with paclitaxel liposome and radiotherapy simultaneously on serum CYFRA21-1, CEA, SCCA, CA125, IL-8 and T lymphocyte subsets in patients with advanced cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Wen-Jing Yang; Lu Wang

    2016-01-01

    Objective:To study the effect of endostar combined with paclitaxel liposome and radiotherapy simultaneously on serum CYFRA21-1, CEA, SCCA, CA125, IL-8 and T lymphocyte subsets in patients with advanced cervical cancer.Methods:A total of 72 patients with advanced cervical cancer in our hospital from January 2014 to February 2016 were enrolled in this study. The subjects were divided into control group (n=36) and experiment group (n=36) randomly. The control group were treated with radiotherapy, the experiment group were treated with endostar combined with paclitaxel liposome and radiotherapy simultaneously. 3 weeks for a period of treatment and the two groups were treated for 4 periods. The serum CYFRA21-1, CEA, SCCA, CA125, IL-8 levels and peripheral blood CD3+, CD4+ and CD8+ cells of the two groups before and after treatment were compared.Results:There were no significantly differences of the serum CYFRA21-1, CEA, SCCA, CA125, IL-8 levels and peripheral blood CD3+, CD4+ and CD8+ cells of the two groups before treatment. The serum CYFRA21-1, CEA, SCCA, CA125 and IL-8 levels of the two groups after treatment were significantly lower than before treatment, and that of experiment were significantly lower than control group. The peripheral blood CD3+, CD4+ cells of the two groups after treatment were significantly lower than before treatment, CD8+ cells of the two groups after treatment were significantly higher than before treatment, and that of experiment group were significantly better than control group.Conclusion: Endostar combined with paclitaxel liposome and radiotherapy simultaneously can significantly reduce the serum CYFRA21-1, CEA, SCCA, CA125 and IL-8 levels, improve peripheral blood CD3+, CD4+ and CD8+ levels of patients with advanced cervical cancer, and it was worthy clinical application.

  14. Performance of biomarkers SMRP, CA125, and CYFRA 21-1 as potential tumor markers for malignant mesothelioma and lung cancer in a cohort of workers formerly exposed to asbestos

    Energy Technology Data Exchange (ETDEWEB)

    Gube, M. [RWTH Aachen University, Institute for Occupational and Social Medicine, Medical Faculty, Aachen (Germany); Westfaelische Technische Hochschule, Institut fuer Arbeitsmedizin und Sozialmedizin am Universitaetsklinikum Aachen, Rheinisch, Aachen (Germany); Taeger, D.; Weber, D.G.; Pesch, B.; Johnen, G.; Gross, I.M.; Wiethege, T.; Weber, A.; Bruening, T. [Institute of the Ruhr-Universitaet Bochum (IPA), Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Bochum (Germany); Brand, P.; Mueller-Lux, A.; Kraus, T. [RWTH Aachen University, Institute for Occupational and Social Medicine, Medical Faculty, Aachen (Germany); Raithel, H.J. [University of Erlangen-Nuremberg, Institute for Occupational, Social and Environmental Medicine, Erlangen-Nuremberg (Germany)

    2011-03-15

    The aim of the study is to examine the cancer-predictive values of SMRP (soluble mesothelin-related peptides), CA125, and CYFRA21-1 as potential tumor markers for lung cancer and malignant mesothelioma in a cohort of workers formerly exposed to asbestos. A voluntary surveillance program has been established for German workers with former asbestos exposure. A subgroup of 626 subjects with a mean age of 63 years (range 53-70 years) at baseline was enrolled in an extended health examination program with high-resolution computer tomography (HRCT) of the chest and blood drawing between 1993 and 1997. Serum concentrations of SMRP, CA125, and CYFRA21-1 were measured in archived serum samples in 2005 and 2006. A mortality follow-up was conducted through 2007. So far, 12 cases with lung cancer and 20 cases with malignant mesothelioma have been observed in this cohort. The average time between sample collection and diagnosis was 4.7 years. Analyzed biomarkers showed low sensitivities (5-25%) and positive predictive values (4-30%) for both cancer sites. Marker combinations resulted in sensitivities between 5 and 50% and positive predictive values ranging from 3 to 14%. Even in those cases, where biomarker concentrations were available within 36 months before diagnosis, no trend for increasing biomarker levels was observed. The analyzed tumor markers were characterized by high specificities, but low sensitivities. SMRP, CA125, and CYFRA21-1 alone or in combination were less suitable to serve as predictors for the diagnosis of lung cancer or malignant mesothelioma. However, a prospective study with annual sampling might reveal a better predictive value of these markers. (orig.)

  15. Correlation between CYFRA21-1 , pathology type and smoking in lung cancer patients%肺癌患者血清细胞角蛋白19片段与病理类型及吸烟的相关性分析

    Institute of Scientific and Technical Information of China (English)

    赵连爽; 赵俊华; 任慧雯; 赵玉霞; 丁丝露; 曹丽艳; 门桐林; 范增慧

    2011-01-01

    目的:探讨肺癌患者血清肿瘤标记物细胞角蛋白19片段(CYFRA21-1)与肿瘤病理类型和吸烟的相关性和对肺癌的诊断价值.方法:应用化学发光技术测定经病理确诊的187例肺癌患者、33例肺良性疾病和健康对照的CYFRA21-1水平.应用SPSS18统计软件包分析病理类型、吸烟和CYFRA21-1间的关系.结果:187例肺癌患者血清中CYFRA21-1水平明显高于非良性病和健康对照组,肺良性病与健康对照组比无显著差别;187例患者吸烟者123例(65.7%),不吸烟者64例(34.2%).在肺鳞癌、肺腺癌及小细胞肺癌中,吸烟者分别为72.9%、52.3%和73.0%,各组间行卡方分析χ2= 8.03 P=0.018;血清CYFRA21-1在鳞癌、腺癌和小细胞肺癌的水平分别为13.96±22.84、7.12±12.22、3.66±4.66,各组间有显著差别(F=5.58,P=0.004);阳性率分别为58.8%、38.5%和32.4%,χ2=9.82,P=0.007,但腺癌与小细胞癌比无显著差别.cfyra211阳性率在非吸烟组、轻、中、重度吸烟组分别为39.1%、61.5%、52.2%和43.0%,四组间阳性率有显著差别,以非吸烟组为最低,重度吸烟组次之(χ2=9.542 P=0.023).结论:c fyra211在肺鳞癌、腺癌和小细胞癌中肺鳞癌的水平和阳性率最高;肺鳞癌和小细胞癌吸烟者占大多数,cfyra211水平与吸烟无明显的相关性,但对肺鳞癌的诊断有一定的价值.%Objective:To investigate the correlation between CYFRA21 - 1 , pathology type of tumor and smoking and to investigate the diagnosis value in lung cancer of the CYFRA21 - 1 in lung cancer patients.Methods: CYFRA21 -1 level was detected by chemoluminescence in 187 lung cancer patients,33 lung disease and healthy persons.Relationships were analyzed by SPSS18 statistics software among pathology type, smoking and CYFRA21 -1.Results:In the blood serum of 187 lung cancer patients, the CYFRA21 -1 level is higher than the control groups of non - benign diseases and healthy patients obviously, compared no remarkable difference between

  16. The clinical significance of serum SCC-Ag combined with CD105 in patients with cervical cancer during the early stage diagnosis

    Institute of Scientific and Technical Information of China (English)

    Ru-Chan Ma; Hong Bi; Sen-Yang Cao

    2016-01-01

    Objective:To invest the clinical significance of serum SCC-Ag combined with CD105 in early diagnosis of cervical cancer to provide new ideas for early diagnosis and clinical treatment of cervical cancer.Methods: A total of 74 cases cervical cancer patients were selected as cervical cancer group, and 52 cases uterine fibroids patients were selected as normal cervical group, serum samples were collected in the early morning fasting condition, SCC-Ag and CD105 were checked by ELISA method, SCC-Ag and CD105 of two groups were analyzed by t-test, and to compare SCC-Ag and CD105 in different TMN staging, lymph gland metastasis and non-lymph gland metastasis in patients with cervical cancer, the correlation analysis was used by Pearson correlation analysis method.Results: These results came from ELISA method, comparing with normal cervical group, the SCC-Ag and CD105 of cervical cancer group increased, the difference was statistically significant. Comparing withⅠperiod of TMN staging, SCC-Ag and CD105 ofⅡ period increased,Ⅲ,Ⅳ period increased, the difference was statistically significant. Comparing withⅡ period, SCC-Ag and CD105 ofⅢ,Ⅳ period increased, the difference was statistically significant. Comparing with non-lymph gland metastasis, SCC-Ag and CD105 of lymph gland metastasis increased in cervical cancer with surgical treatment, the difference was statistically significant. According to Pearson correlation analysis, SCC-Ag and CD105 were positively correlated.Conclusion: SCC-Ag and CD105 in patients with cervical cancer increase highly, it has important clinical value that of serum SCC-Ag combined with CD105 in the early diagnosis of cervical cancer, especially it has clinical guiding significance to staging and lymph gland metastasis of cervical cancer, and it is worthy of clinical reference.

  17. The relationship between circulating tumor cells counting and Cyfra21 -1,CEA,Fg%循环肿瘤细胞数目与血清中 Cyfra21-1、CEA、Fg 表达水平的关系

    Institute of Scientific and Technical Information of China (English)

    陈可绪; 陈东杰; 李毅斌; 张昱; 汪森明

    2015-01-01

    目的:探讨肿瘤患者外周血循环肿瘤细胞(CTCs)与常用的肿瘤指标细胞角质素19可溶片段(Cy-fra21-1)、癌胚抗原(CEA)及凝血功能指标纤维蛋白原(Fg)表达水平的关系,比较 CTCs 阳性组和阴性组转移灶数目差异。方法:采集66例进展期晚期肺癌住院患者治疗前7.5ml 外周血,保存于4℃冰箱中,24小时内由益善生物技术公司使用“免疫去除结合纳米过滤法”行 CTCs 检测。同时检测血清中 Cyfra21-1、CEA、Fg水平,收集并分析 CTCs 数目与转移灶数目、临床 T 分期、转移淋巴结的关系。结果:纳入患者中 CTCs 检出率为86.4%。CTCs 数目与 Cyfra21-1水平有相关关系,相关系数为0.365(P =0.003);CTCs 数目与 Fg 水平相关,相关系数为0.330(P =0.007);CEA 与 CTCs 两变量无明显相关性。两组患者的肿瘤转移器官数目大于3个的比例有显著差异。结论:两组患者 Cyfra21-1异常增高率有显著差异,且 CTCs 数目与 Cyfra21-1表达水平正相关,可由 Cyfra21-1初步估计 CTCs 水平。而外周血中 Fg 异常增高率无显著差异,但二者表达水平呈明显正相关,因此分析 CTCs 数目需要考虑 Fg 表达水平的影响。%Objective:To investigate the relevance between circulating tumor cells(CTCs)counting and the fol-lowing tumor markers:Cyfra21 -1,CEA,Fg,and compare the survival,sites of metastasis organs among patients strati-fied by CTCs counting.Methods:We selected 66 histologically diagnosed advanced lung cancer patients of progression period,applied CanpotrolTM,which developed by SurExam biotechnology company to detect CTCs in the blood,7. 5ml blood was needed before treatment for CTCs test.All the samples were stored at 4 cent degree after collection,and samples were tested within 24h.Serum concentration of Cyfra21 -1,CEA,Fg were detected to investigate the correla-tion of CTCs counting and tumor

  18. Methodological study on magnetic affinity immunoassay for detecting SCC-Ag%磁分离酶联免疫法检测SCC-Ag的方法学研究

    Institute of Scientific and Technical Information of China (English)

    周敏; 陆仁飞; 杨旻

    2014-01-01

    目的:建立检测鳞状上皮细胞癌抗原(squamous cell carcinoma antigen,SCC-Ag)的磁分离酶联免疫法(mag-netic affinity immunoassay,MAIA)。方法:采用双抗体夹心法,用异硫氰酸荧光素(fluorescein isothiocyanate,FITC)和碱性磷酸酶(alkaline phosphatase,AP)分别标记识别不同表位的两种抗 SCC-Ag 单克隆抗体(mAb) T17和 H390,以耦联羊抗FITCmAb包被的免疫磁珠作为固相载体,APS-5作为显色底物,建立检测SCC-Ag的磁分离酶联免疫法。结果:成功实现了用磁分离酶联免疫法对SCC-Ag的定量检测,其检测灵敏度为0.22μg/mL,线性范围0~50μg/mL,批内CV 7%~8%,批间CV 6%~9%。添加回收率为97%。与雅培AxSYM微粒子化学发光检测结果对比,相关系数为0.973。结论:磁分离酶联免疫法的检测结果接近进口同类试剂的检测结果,为开发一种高质量的SSC-Ag测定试剂盒提供实验数据。%Objective:To establish magnetic affinity immunoassay(MAIA) for detecting SCC-Ag. Methods: The immunoassay was based on antibody sandwich immunoassay, two monoclonal antibodies T17,H390 were used for conjugating with alkaline phosphatase and with fluorescein isothiocyanate ( FITC ) respectively , which incorporated magnetic solid phase separation . Magnetic beads were coupled with sheep anti-FITC antibody as solid phase , and APS-5 was used as a substrate to set up MAIA for detecting SCC-Ag. Results: Established the MAIA for detecting SCC-Ag successfully. The sensitivity of the MAIA method was 0.22 μg/mL, the linear range was 0-50 μg/mL, the intra-and inter-assay variation were 7%-8% and 6%-9%respectively. The addition recovery rate was 97%. The correlation coefficient was 0.973 in comparison with Abbott AxSYM. Conclusion: SCC-Ag MAIA kit is better than SCC-Ag RIA and ELISA kit, which can provide experimental data for the de-velopment of a high quality of the SSC-Ag assay kit.

  19. Clinical application of combined detection of tumor molecular markers in lung cancer%肿瘤分子标志物联合动态检测在肺癌中的临床应用

    Institute of Scientific and Technical Information of China (English)

    徐日; 徐风亮

    2014-01-01

    目的 研究肿瘤分子标忐物癌胚抗原(CEA)、糖类抗原(CA125)、细胞角蛋白19片段抗原21-1(CYFRA21-1)、鳞状上皮癌抗原(Scc-Ag)、神经元特异性烯醇化酶(NSE)、胃泌素释放肽前体31-98(Pro-GRP31-98)联合动态检测在肺癌诊断、临床分期、组织学分型及治疗监控中的临床应用价值.方法 对198例肺癌患者(肺癌组)、50例肺部良性病变患者(良性对照组)及50名健康体检者(健康对照组)采用电化学发光法检测血清CEA、CA 125、CYFRA21-1、Scc-Ag、NSE,酶联免疫法检测Pro-GRP31-98水平,分析肿瘤分子标志物与肺部良恶性疾患的相关性.结果 肺癌组患者血清CEA、CA125、CYFRA21-1、Scc-Ag、NSE、Pro-GRP31-98水平与肺部良性病变组及健康对照组比较差异有统计学意义,P< 0.01;肺癌高分期(Ⅲ、Ⅳ期)组血清肿瘤分子标志物水平明显高于肺癌低分期(Ⅰ、Ⅱ期)组,治疗前、淋巴结转移组、复发组与治疗后、无转移组、无复发组比较血清肿瘤标志物水平明显增高,组间分别比较差异有统计学意义,均P< 0.01;CEA、CA125分别对肺腺癌的阳性榆出率与鳞状细胞癌、小细胞癌比较差异有统计学意义,P< 0.01;CYFRA21-1、Scc-Ag分别对肺鳞状细胞癌的阳性检出率与腺癌、小细胞癌比较差异有统计学意义,P< 0.01;NSE、Pro-GRP31-98分别对肺小细胞癌的阳性检出率与腺癌、鳞状细胞癌比较差异有统计学意义,P< 0.01;CEA联合CA125、CYFRA21-1联合Scc-Ag、NSE联合Pro-GRP31-98检测分别对肺腺癌、鳞状细胞癌、小细胞癌阳性检出率较单项检测比较明显提高,均P< 0.01;CEA、CA125、CYFRA21-1、Scc-Ag、NSE、Pro-GRP31-98诊断肺癌的敏感性分别为52.52%、51.01%、48.99%、53.03%、49.49%、50.50%,六项肿瘤标志物联合检测诊断肺癌的敏感性高达96.46%,与各单项检测比较差异有统计学意义(P<0.01).结论 肿瘤分子标志物CEA、CA125、CYFRA

  20. Effect of renal function and hemodialysis on the serum tumor markers in patients with chronic kidney disease

    Institute of Scientific and Technical Information of China (English)

    YU Xiaofang; XU Xialian; YE Zhibin

    2007-01-01

    In patients with chronic renal failure,whether they have had hemodialysis or not,the specificity of some of the serum tumor markers for the diagnosis of the corresponding tumors is decreased while others remain as valuable as they are in patients with norrnal kidney function.The detection of tumor markers is extensively used for the diagnosis of corresponding tumors.It has been recently shown that some tumor markers are higher in patients with chronic kidney disease(END)than in the normal population.The effects of renal function and hemodialysis were examined on serum levels of some of the tumor markers including CEA,CA199,CA125,AFP,CA153,CA724,CYFRA21-1,NSE,SCC-Ag,PSA,and fPSA.The 232 non-dialysis patients with CKD and 37 chronic uremic patients treated with maintenance hemodialysis were enrolled in this study.The 232 non-dialysis patients were divided into three groups according to their Ccr.In group 1,Ccr was≤25 mL/min.In group 2,Ccr was between 25 and 50 mL/min.In group 3,Ccr was≥50 mL/min.The male patients were also divided into three groups to compare the serum levels of PSA and fPSA among the three groups.Nine tumor markers in 37 uremic patients were tested.For comparison.37 non-dialysis patients with similar Ccr of the same age and gender served as controls.There existed significant difierences in serum levels of CEA,CA199,CYFRA21.1,NSE,and SCC-Ag among different Ccr groups and the markers bore a negative correlation with Ccr.There were no significant differences among the three groups in the serum concentrations of CA125,AFP,CA153,CA724,PSA and fPSA.The serum levels of CA125 and NSE were significantly higher(P<0.01)in hemodialysis patients than in the nondialysis control patients.In patients with chronic renal failure,who were or were not on hemodialysis,the specificity of serum CEA,CA199,CYFRA21-1,NSE,CA125 and SCC-Ag for the diagnosis of the corresponding tumors was decreased while serum AFP,CA153,CA724,PSA and fPSA were as valuable as they were in

  1. The Clinical Value of 6 Serum Tumor Markers in Lung Cancer Diagnosis%血清肿瘤标志物单项及联合检测在肺癌诊断中的临床价值

    Institute of Scientific and Technical Information of China (English)

    方东风; 汪德明

    2012-01-01

    目的 探讨血清癌胚抗原(Carcinoembryonic antigen,CEA)、糖类抗原19-9(CA19-9)、糖类抗原125(CA125)、细胞角蛋白19片断(Cytokeratinfragment 19,CYFRA21-1)、神经特异性烯醇化酶(Neuron-specific enolase,NSE)、鳞状细胞抗原(SCC-Ag)6种肿瘤标志物单项及联合检测在肺癌诊断中的临床价值.方法 采用化学发光免疫法检测92例肺癌患者、92例肺良性疾病患者、92例健康体检者的血清CEA、CA19-9、CA125、CYFRA21-1、NSE、SCC-Ag表达水平.结果 肺癌患者的血清肿瘤标志物CEA、CA19-9、CA125、CYFRA21-1、NSE、SCC-Ag的表达均明显高于肺良性疾病患者和健康体检者(P均<0.05);6种肿瘤标志物对肺癌诊断的灵敏性和准确度分别为:CEA(51.1%、73.3%)、CYFRA21-1(58.7%、73.9%)、CA125(38.0%、71.4%))、CA19-9(27.2%、70.3%)、NSE(26.1%、65.9%)、SCC-Ag(35.9%、71.0%),而六者联合检测的灵敏性和准确度分别为92.4%和83.7%,明显高于各单项检测(P<0.05).结论 血清肿瘤标志物CEA、CA19-9、CA125、CYFRA21-1、NSE、SCC-Ag是诊断肺癌较好的标志物,六者联合检测可明显提高肺癌诊断的灵敏性和准确度.%Objective To investigate the Clinical values in lung cancer diagnosis to combine six serum tumor markers CEA,CYFRA21-l,NSE,SCC-Ag,CA19-9and CA125. Methods To analyze retrospectively the diagnosis sensititivity and specificity of CEA,CYFRA21-l,NSE,SCC-Ag,CA19-9 and CA125 in 92 lung cancer patients, 92 benign lung disease and 92 health a-dults. Results The serum tumor marker levels in the lung cancer group were obviously higher than those in health adults group and benign lung disease group ( P <0. 05 );These six serum tumors combinations may be more sensitive and helpful in the diagnosis of lung cancer. Conclusion Combination of multiple tumor markers can increase the clinical diagnosisvalue in lung cancer.

  2. The clinical value of serum tumor markers combined dynamic detection in early diagnosis and monitoring treatment of lung cancer%血清肿瘤标记物联合动态检测在肺癌早期诊断和监控治疗中的临床价值

    Institute of Scientific and Technical Information of China (English)

    邹见刚; 王笑峰

    2016-01-01

    测比较差异有统计学意义(均<0.01)。结论:血清肿瘤标记物CA50、CEA、Scc- Ag、CYFRA21-1、PTN、NSE联合动态检测可作为肺癌早期诊断的辅助手段及疾病复发转移和治疗有效性的监控指标,有利于临床早期诊断、指导治疗、判断预后。%Objective: To investigate the clinical application value of serum tumor marker carbohydrate antigen CA50, carci-noembryonic antigen (CEA), squamous cell carcinoma antigen (Scc- Ag), antigen of cytokeratin 19 fragment (CYFRA21- 1), people multi- effect PTN protein and neuron specific enolization enzyme NSE joint dynamic testing in the early diagnosis and monitoring of lung cancer. Methods: Serum tumor marker levels of CA50、 CEA、 Scc- Ag、 CYFRA21- 1、 PTN、 NSE were measured with electrochemiluminescence and ELISA respectively in 201 patients with lung cancer and 50 patients with lung benign lesions and 50 cases of healthy physical examination. Retrospective statistical methods were used to analyze the correlation of the serum tumor mark-ers and benign and malignant lung disease, and futher to explore the early diagnosis and monitoring clinical value in lung cancer by serum tumor markers joint dynamic detection treatment. Results:(1)Serum CA50, CEA, Scc- Ag, CYFRA21- 1, PTN, NSE levels in lung cancer group were significantly higher than the group of lung benign disease and normal control ( 0.05), but all were significantly related with tumor size, clinical stage, histological type, recurrence and metasasis, combining pleural effusion and post treatment(all <0.01); (3)Serum CA50 and CEA had higher expression levels and positive detec-tion rate in lung adenocarcinoma patients; Scc- Ag and CYFRA21- 1 had higher serum expression levels and positive detection rate in lung squamous carcinoma patients; PTN and NSE had higher serum expression levels and positive detection rate in small cell lung cancer patients (all <0.01) ; (4) The sensitivity of CA50, CEA, Scc Ag

  3. 血清SCC-Ag、VEGF联合检测在宫颈癌诊断中的意义%Value of joint detection of serum SCC-Ag and VEGF in the diagnosis of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    王卉

    2015-01-01

    目的:联合检测子宫颈癌患者血清中血清鳞状细胞癌抗原(SCC-Ag)和抗血管内皮生长因子(VEGF)水平,探讨其临床意义及两者的相关性。方法通过酶联免疫吸附试验法(ELISA)检测宫颈癌30例,宫颈上皮内瘤变(CIN)患者30例和正常人30名血清中SCC-Ag和VEGF的水平。结果宫颈癌组患者血清SCC-Ag和VEGF水平及阳性率均高于上皮内瘤变组及正常组,上皮内瘤变组高于正常组,差异有统计学意义(P<0.05)。结论联合检测宫颈癌患者血清中SCC-Ag和VEGF水平有助于判断患者的病情及预后,在临床上必将有着广阔的应用前景。%ObjectiveTo detect the levels of serum SCC-Ag and VEGF in cervical cancer and investigate their correlation and clinical application.MethodsThe levels of serum SCC-Ag and VEGF were measured by ELISA in 30 cases of healthy individuals, 30 cases of cervical intraepithelial neoplasia and 30 cases of cervical cancer. ResultsThe levels and positive rate of serum SCC-Ag and VEGF of group cervical cancer were signifi cantly higher than those of group cervical intraepithelial neoplasia and group healthy individuals, there were signifi cant differences between the three groups (P<0.05).ConclusionThe joint detection of serum SCC-Ag and VEGF has signifi cant diagnostic value for early stage cervical cancer.

  4. Serum squamous cell carcinoma antigen and CYFRA 21-1 in cervical cancer treatment

    NARCIS (Netherlands)

    Pras, E; Willemse, PHB; Canrinus, AA; de Bruijn, HWA; Sluiter, WJ; ten Hoor, KA; Aalders, JG; Szabo, BG; de Vries, EGE

    2002-01-01

    Purpose: To analyze whether serum squamous cell carcinoma (SCC) antigen and cytokeratin-19 fragments (CYFRA) levels can assist in selecting patients with locally advanced cervical cancer who will benefit from combined treatment or additive surgery. Methods and Materials: Of 114 patients with cervica

  5. 肿瘤标志物在肺癌诊断、病理分型和临床分期中的价值%Value of detection of serum tumor biomarkers in lung cancer diagnosis, pathological type and clinical stage

    Institute of Scientific and Technical Information of China (English)

    李慧; 陈艳乐; 常静侠; 张国俊

    2013-01-01

    Objective To evaluate the efficiency of 5 tumor biomarkers,namely carbohydrate antigen125 (CA-125),carbohydrate antigen-199 (CA-199),carcinoembryonic antigen (CEA),neuron-specific enolase (NSE),Cytokeratin 19 (CYFRA21-1),independently or in combination for lung cancer diagnosis,pathological type and clinical stage.Methods Electrochemiluminesce-nce immunization was used to determine biomarker levels expressed in 309 patients with pulmonary disease.Results The observed levels of CA125,CA199,CEA,NSE and CYFRA 21-1 in patients with pathologically confirmed lung cancer were significantly higher than those in patients with benign pulmonary diseases.Adenocarcinoma,squamous cell carcinoma,and small cell lung carcinoma of the lung were associated with the highest sensitivity levels of CYFRA21-1 and CEA,CYFRA21-1,and NSE,respectively.The combination of 4 tumor biomarkers,namely,CA125 + CEA +NSE + CYFRA21-1 showed 87.26% sensitivity for lung cancer.The ROC of CYFRA21-1 was 0.840 ± 0.032(95% CI,0.777-0.904).The positive rates and levels of CA125,CEA,CYFRA21-1 were different in clinical stages of lung cancer.Conclusion CA125,CA199,CEA,NSE and CYFRA 21-1 are valuable in the diagnosis,pathological type and clinical stage of lung cancer,especially CYFRA 21-1.The combination of CA125 + CEA + NSE + CYFRA21-1 showed higher sensitivity for lung cancer.CA125,CEA,CYFRA21-1 play an important role in clinical stage.%目的 研究血清肿瘤相关抗原125(CA125)、199(CA199)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA21-1)在肺癌诊断、病理分型和临床分期中的价值.方法 选取郑州大学第一附属医院2011年6月至2012年9月入院患者309例血清标本,采用电化学免疫荧光发光法检测血清肿瘤标志物的浓度.结果 肺癌患者5种血清肿瘤标志物水平均高于肺部良性病变组.单项检测,腺癌、鳞癌、小细胞肺癌(SCCL)敏感度最高者分别为CYFRA21-1和CEA、CYFRA21-1NSE

  6. 胸腔热灌注治疗肺癌恶性胸腔积液前后肿瘤标记物的变化研究%The Change of Levels of Tumor Markers in Lung Cancer Patients With Malignant Pleural Effusion Before and After Intrapleural Hyperthermic Perfusion

    Institute of Scientific and Technical Information of China (English)

    董彦军; 郑先杰; 张国瑜; 孙明飞; 杨忠信; 张双林

    2016-01-01

    Objective To investigate the changes of tumor markers of lung cancer before and after the treatment of lung cancer with pleural effusion. Methods 62 cases of lung cancer with malignant pleural effusion were randomly divided into two groups. The treatment group was treated by pleural perfusion for 3 times, 1 h, 24 h interval. The control group was perfused with cisplatin after closed thoracic drainage. Detection of the ifrst day, the 30th day of serum carcinoembryonic antigen (CEA), neuron speciifc ene alcoholize (NSE), cytokeratin protein 19 fragment (CYFRA21-1), squamous epithelial cell carcinoma antigen (SCC-AG) level, Combined with imaging evaluation. Results The total effective rate (RR) of the treatment group was 96.4%(PR 92.1%+CR 4.3%). The total effective rate of the control group (RR) was 54.2%(PR 39.1%+CR 15.1%). After 30 d treatment, the treatment group and the control group CEA, NSE, CY-FRA21-1 were significantly decreased, compared with before treatment, the difference was statistically signiifcant (P<0.05). And there was signiifcant difference between the treatment group and the control group (P<0.05). Conclusion Treatment of malignant pleural effusion of lung cancer with pleural effusion, CEA, NSE, CY-FRA21-1, SCC-AG can be used as the evaluation index to evaluate the therapeutic effect of lung cancer with malignant pleural effusion.%目的探讨胸腔热灌注治疗肺癌胸腔积液前后肺癌肿瘤标记物的变化,评估热灌注治疗的疗效。方法62例肺癌恶性胸腔积液患者,随机分成两组。治疗组经过胸腔热灌注治疗3次,持续1 h,间隔24 h。对照组在胸腔闭式引流术后灌注顺铂。检测第1天、第30天血清癌胚抗原(CEA)、神经元特异性烯醇化(NSE)、细胞角质蛋白19片段(CYFRA21-1)、鳞状上皮细胞癌抗原(SCC-AG)水平,结合影像学进行评价。结果治疗组胸液控制总有效率(RR)为96.4%(CR 92.1%+PR 4.3%),对照组胸液

  7. Research status of SCC - Ag and Survivin on cervical and other squamous cell carcino-mas%宫颈及其他鳞癌 SCC - Ag、Survivin 研究现状

    Institute of Scientific and Technical Information of China (English)

    陈瑜; 王振国; 李争

    2015-01-01

    Cervical cancer,with high morbidity and mortality,is the third most commonly diagnosed cancer in fe-males worldwide. Persistent infection of high - risk human papillomavirus(HR - HPV)is its major risk factor. Squa-mous cell carcinoma(SCC),which is not only the most important pathological type of cervical cancer,but also exists in skin,head and neck,esophagus,lung and so on,is a serious threat to human health and life. As a new mean,bio-marker along with targeted therapy is of importance on the diagnosis and treatment for SCC. Currently,detection of ser-um SCC - Ag alone is used for assisting the diagnosis of SCC,whose sensitivity and specificity can be improved by joint other applications such as examination methods of HPV - DNA,magnetic resonance imaging(MRI)and so on. And these also can help study its carcinogenic mechanism. The roles of Survivin are extensively being studied in SCC in recent years. Especially in the diagnosis during the onset of SCC,Survivin highlights its unique value. And its po-tential applications in targeted therapy have been demonstrated in some further studies.%宫颈癌是目前全球女性第三大常见恶性肿瘤,有较高的发病率和死亡率,高危型 HPV(HR -HPV)持续感染为其主要致病因素。鳞状细胞癌(squamous cell carcinoma,SCC)是宫颈癌最主要的病理类型,也常见于皮肤、头颈、食管、肺等,严重威胁人类健康及生命。作为一种新型手段,生物标记物及靶向治疗对于鳞癌的诊治具有十分重要的意义。血清 SCC - Ag 单独检测目前多用于鳞癌的辅助诊断,联合应用诸如HPV - DNA、磁共振技成像(magnetic resonance imaging,MRI)等检查手段,可提高鳞癌诊断的敏感性与特异性,并有助于其致病机制的研究;生存素在鳞癌中的作用近年来正被广泛研究,尤其于鳞癌发病早期凸显其在诊断中的独特应用价值,更进一步研究展示出了其在靶向治疗中的潜在应用前景。

  8. Application value of combination detection of tumor markers in diagnosis and differential diagnosis of lung cancer%肿瘤标志物联合检测在肺癌诊断及鉴别诊断中的应用价值

    Institute of Scientific and Technical Information of China (English)

    李娜; 李国华

    2014-01-01

    目的:探讨血清神经元特异性烯醇化酶(NSE)、细胞角蛋白 CK19片段(Cyfra21-1)等6种肿瘤标志物在该院肺癌的诊断及鉴别诊断中的应用价值。方法采用电化学发光免疫法检测140例肺癌、57例肺良性疾病患者和45例健康体检者的血清 NSE、Cyfra21-1、糖类抗原19-9(CA19-9)、糖类抗原12-5(CA12-5)、血清鳞状细胞癌抗原(SCC-Ag)、癌胚抗原(CEA),观察这6种肿瘤标志物在肺癌的诊断及肺癌与肺良性疾病鉴别诊断中的价值。结果肺癌组 CA12-5、CA19-9、CEA 水平均显著高于肺良性疾病组及健康体检组(P <0.05);6种肿瘤标志物联合检测的灵敏度和准确度分别为95.2%、89.7%,均优于单项检测。结论肿瘤标志物需联合检测可提高肺癌诊断的灵敏度和准确度,有助于肺癌早期的鉴别诊断。%Objective To investigate the application value of 6 kinds of tumor markers serum neuron-specific enolase(NSE),cy-tokeratin 19 fragment(Cyfra21-1),carbohydrate antigen 19-9(CA19-9),carbohydrate antigen 12-5(CA12-5),squamous cell carcino-ma antigen(SCC-Ag),carcino embryonic antigen(CEA)in the diagnosis and differential diagnosis of lung cancer.Methods Serum NSE,Cyfra21-1,CA19-9,CA12-5,SCC-Ag and CEA levels were detected in 140 cases of lung cancer,57 cases of benign lung disea-ses and 45 individuals with healthy physical examination as control group by electrochemiluminescence immunoassay.The values of these 6 kinds of tumor marker in the diagnosis and the differential diagnosis of lung cancer were observed.Results The levels of CA12-5,CA19-9 and CEA in the lung cancer group were significantly higher than those in the benign lung diseases group and the healthy physical examination group(P <0.05 ),the sensitivity and accuracy in the combined detection of the six kinds of tumor marker in diagnosing lung cancer were 95.2% and 89.7% respectively,which was superior to those in single index

  9. Clinical value of combined detection of serum tumor markers in patients with lung cancer%肿瘤标志物联合检测在肺癌诊断中的应用价值

    Institute of Scientific and Technical Information of China (English)

    张宝秋; 丁湘彧; 王雪玉; 张洁; 时广利

    2012-01-01

    目的 探讨血清肿瘤标志物癌胚抗原(CEA)、胃泌素释放肽前体(pro-GRP)、神经特异性烯醇化酶(NSE)、鳞状上皮细胞癌抗原(SCC-Ag)、细胞角蛋白19片段(CYFRA21-1)对肺癌辅助诊断的临床价值,并选择较理想的肿瘤标志物组合.方法 应用ELISA法检测50例健康者、90例肺部良性疾病患者和122例肺癌患者血清中5种肿瘤标志物水平.结果 肺癌患者血清中5种肿瘤标志物水平明显高于健康组和良性肺部疾病组(P<0.01);CEA在腺癌中的检测水平明显高于其他类型肺癌(P<0.01);NSE、pro-GRP在小细胞肺癌(SCLC)中的检测水平明显高于其他类型肺癌(P<0.01);SCC-Ag在鳞癌中的检测水平明显高于其他类型肺癌(P<0.05);CYFRA21-1在鳞癌中的检测水平明显高于其他类型肺癌(P<0.01);5种肿瘤标志物组合后,敏感性明显高于单项肿瘤标志物的敏感性(P<0.01).结论 5种肿瘤标志物在肺癌的辅助诊断和鉴别诊断中有着重要的临床意义.NSE、pro-GRP可作为联合检测小细胞肺癌的标志物组合,CEA、SCC-Ag和CYFRA21-1可作为联合检测非小细胞肺癌的标志物组合.%Objective To evaluate the clinical significance of tumor markers, including carcinoembryonic antigen(CKA) , precursor of gastrin-releasing peptide(pro-GRP) , neurone specific enolase(NSE) ,squamous cell carcinoma antigen(SCC-Ag) and cytoker-atin-19-fragments(CYFRA21-l) ,in single or combined detection,for the diagnosis of lung cancer. Methods Serum levels of the five serum tumor markers were detected by ELISA in 50 healthy adultsChealthy group) ,90 patients with benign lung disease (benign group) and 122 patients with lung cancerCcancer group). Results Serum levels of the five serum tumor markers in cancer group were significantly higher than those in healthy group and benign group(P<0. 01). According to different subtypes of lung cancer, serum level of CEA was the most significantly high in patients with adenocarcinoma(P<0

  10. 血浆长链非编码RNA转移相关肺腺癌转录因子1对肺癌临床诊断价值%Diagnostic value of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 in serum for clinical diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    智能; 李霞

    2016-01-01

    Objective To explore diagnostic value of long non-coding RNA metastasis-associated lung ade-nocarcinoma transcript 1 (MALAT1) in serum for clinical diagnosis of lung cancer. Methods Serum MALAT1, CEA ( Carcinoembryonic antigen) , CYFRA21-1 ( cytokeratin 19 fragments) , SCC-Ag ( Squamous cell carcinoma an-tigen) and NSE (neuron specific enolase) levels were detected in 84 lung cancer cases (34 adenocarcinoma carcino-ma, 26 squamous carcinoma cases, 24 small cell lung cancer cases) and 60 healthy people. The related indexes, such as sensitivity and specificity, were analyzed by logistic regression and ROC curves. Results The serum MAL-AT-1 level in lung cancer cases were obviously higher than in healthy controls (P<0. 01). When the cutoff point was 0. 03, the positive rate of MALAT-1 was 70. 95%, and the area under the ROC curve (AUC) of MALAT-1 in lung cancer were 0. 70, and the sensitivity and specificity were 58. 33% and 81. 67%, respectively. The AUC of the com-bined determination of MALAT-1, NSE and CYFRA21-1 in lung cancer and small cell lung cancer cases were 0. 91 and 0. 98, respectively. The sensitivity was 82. 14% and 95. 83%, and the specificity was 85. 00% and 93. 33% re-spectively. The AUC, sensitivity, and specificity of MALAT-1 in adenocarcinoma were 0. 81, 76. 47%, and 83. 33%, respectively. Conclusion MALAT-1 can be regarded as an independent serological diagnostic tumor marker of lung cancer, especially lung adenocarcinoma. The combined determination of MALAT-1, NSE and CY-FRA21-1 can significantly increase the diagnosis effects of lung cancer and different pathological types of lung cancer.%目的:探讨血浆中长链非编码RNA转移相关肺腺癌转录因子1(MALAT1)对肺癌临床诊断意义。方法分别检测84例肺癌患者(34例腺癌、26例鳞癌、24例小细胞性肺癌)和60例体检健康者血浆中MALAT1、癌胚抗原(CEA)、CK19片段(CYFRA21-1)、鳞状细胞癌抗原(SCC-Ag)和神经元特异性烯醇化酶( NSE

  11. 血清SccAg、CA125、CA19-9在宫颈癌及癌前病变中的表达及意义%Expressions and significances of serum SccAg, CA125, and CA19 -9 in cervical cancer and cervical precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    甘玉杰; 熊小英; 符丽华; 谭玲玲

    2012-01-01

    Objective: To study the expressions and significances of serum squamous cell carcinoma antigen (SccAg) , cancer antigen 125 (CA125) , carbohydrate antigen 19-9 (CA19 -9) in cervical cancer and cervical precancerous lesion. Methods; Thirty-four serum samples in normal control group, one hundred and twenty - six serum samples in cervical intraepithelial neoplasia (CIN) group, and fifty - seven serum samples in cervical cancer group were detected, the three tumor markers in cervical cancer of different clinical stages, differentiation degrees, and pathological types were compared. Results; Serum SccAg expression changed in cervical precancerous lesion, the changes of CA125 and CA19 - 9 were not obvious. There was a positive correlation between serum SccAg level and clinical stages, pathological degrees, and lymph node metastasis of cervical cancer, CA125 and CA19 -9 had great application values in detection of cervical adeno-carcinoma. Conclusion; Comprehensively analyzing tumor markers can provide guidance and data for assisted diagnosis, diagnosis, treatment, and prognosis of the patients with cervical cancer.%目的:研究鳞状上皮细胞癌抗原(SccAg)、癌抗原125 (CA125)、癌抗原19-9 (CA19 -9)在癌前病变及宫颈癌中的表达和意义.方法:检测正常对照组血清34例、宫颈上皮内瘤变(CIN)组126例、宫颈癌组57例,对不同临床分期、分化程度和病理类型宫颈癌的三种肿瘤标志物进行比较.结果:SccAg在宫颈癌前病变已有变化,CA125和CA19 -9变化不明显,血清中SccAg的水平与肿瘤临床分期、肿瘤病理分级、淋巴结转移呈正相关,CA125和CA19 -9在检测腺癌中更有应用价值.结论:综合分析宫颈癌患者的肿瘤标志物能为患者的辅助诊断、病情诊治、预后提供指导信息.

  12. Value of joint detection of serum SCC-Ag, VEGF, IGF-Ⅱ and TK1 in the diagnosis of early stage cervical cancer%血清SCC-Ag、VEGF、IGF-Ⅱ及TK1联合检测在早期宫颈癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    刘淼; 邓玉清; 刘平

    2012-01-01

    Objective To study the diagnostic value of joint detection of serum SCC-Ag, VEGF, IGF-II and TK1 in early stage cervical cancer. Methods Forty-nine patients with early stage cervical cancer from June 2009 to November 2011 were selected as group A, 49 patients with cervical intraepithettal neoplasia at the same time were enrolled as group B, and 49 healthy individuals were enrolled as group C. The levels and positive rate of serum SCC-Ag, VEGF, IGF-II and TK1 were detected and compared between the three groups. Results The levels and positive rate of serum SCC-Ag, VEGF, IGF-II and TK1 of group A were significantly higher than those of group B and group C. which were significantly higher in group B than group C, all with P<0.05. The levels of serum VEGF, IGF-II and TK1 showed statistically significant difference between patients of cervical cancer at I a stage and those in group B (P<0.05). Conclusion Joint detection of serum SCC-Ag, VEGF, IGF-II and TK1 has significantdiagnostic value for early stage cervical cancer.%目的 探讨血清SCC-Ag、VEGF、IGF-Ⅱ及TK1联合检测对早期宫颈癌的诊断价值.方法 选取2009年6月至2011年11月的49例早期宫颈癌患者为A组,同期的49例宫颈上皮内瘤样病变患者为B组,49名健康体检人员为C组,检测并比较三组人员的血清SCC-Ag、VEGF、IGF-Ⅱ及TK1水平.结果 A组血清SCC-Ag、VEGF、IGF-Ⅱ及TK1水平及阳性率均高于B组及C组,而B组则高于C组,宫颈癌Ⅰa期患者与B组则在血清VEGF、IGF-Ⅱ及TK1水平及阳性率方面存在一定的差异,P均<0.05,差异有统计学意义.结论 血清SCC-Ag、VEGF、IGF-Ⅱ及TK1联合检测在早期宫颈癌诊断中有较好的临床价值,对于宫颈癌的早期诊断有着积极的作用.

  13. Monte Carlo simulation of NSE at reactor and spallation sources

    Energy Technology Data Exchange (ETDEWEB)

    Zsigmond, G.; Wechsler, D.; Mezei, F. [Hahn-Meitner-Institut Berlin, Berlin (Germany)

    2001-03-01

    A MC (Monte Carlo) computation study of NSE (Neutron Spin Echo) has been performed by means of VITESS investigating the classic and TOF-NSE options at spallation sources. The use of white beams in TOF-NSE makes the flipper efficiency in function of the neutron wavelength an important issue. The emphasis was put on exact evaluation of flipper efficiencies for wide wavelength-band instruments. (author)

  14. Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex.

    Science.gov (United States)

    Wehrkamp-Richter, Sophie; Hyppa, Randy W; Prudden, John; Smith, Gerald R; Boddy, Michael N

    2012-10-01

    Faithful chromosome segregation in meiosis is crucial to form viable, healthy offspring and in most species, it requires programmed recombination between homologous chromosomes. In fission yeast, meiotic recombination is initiated by Rec12 (Spo11 homolog) and generates single Holliday junction (HJ) intermediates, which are resolved by the Mus81-Eme1 endonuclease to generate crossovers and thereby allow proper chromosome segregation. Although Mus81 contains the active site for HJ resolution, the regulation of Mus81-Eme1 is unclear. In cells lacking Nse5-Nse6 of the Smc5-Smc6 genome stability complex, we observe persistent meiotic recombination intermediates (DNA joint molecules) resembling HJs that accumulate in mus81Δ cells. Elimination of Rec12 nearly completely rescues the meiotic defects of nse6Δ and mus81Δ single mutants and partially rescues nse6Δ mus81Δ double mutants, indicating that these factors act after DNA double-strand break formation. Likewise, expression of the bacterial HJ resolvase RusA partially rescues the defects of nse6Δ, mus81Δ and nse6Δ mus81Δ mitotic cells, as well as the meiotic defects of nse6Δ and mus81Δ cells. Partial rescue likely reflects the accumulation of structures other than HJs, such as hemicatenanes, and an additional role for Nse5-Nse6 most prominent during mitotic growth. Our results indicate a regulatory role for the Smc5-Smc6 complex in HJ resolution via Mus81-Eme1.

  15. 鳞状细胞癌抗原(SCC-Ag)在口腔鳞癌患者血清中表达的临床意义%Clinical significance of serum squamous cell carcinoma antigen expression in patients with oral squamous cell carcinoma

    Institute of Scientific and Technical Information of China (English)

    潘凌峰; 李幼华

    2012-01-01

    Serum level of squamous cell carcinoma antigen( SCC- Ag ) in 36 patients with oral squamous cell carcinoma( OSCC ), 11 with other oral and maxillofacial malignant tumor,45 with oral and maxillofacial benign tumor and 70 healthy people was measured by chemolumi-nescence method. Serum level of the SCC-Ag in patients with OSCC was significantly higher than that in those with other malignant tumors( P <0.05 ) or with benign tumors( P < 0. 01 ) or in healthy people( P < 0.01 ). Serum SCC3-Ag level may be an indicator in diagnosis of oral squamous cell carcinoma.%用化学发光法检测36 例口腔鳞癌、11 例口腔颌面部其他恶性肿瘤、45 例口腔颌面部良性肿瘤患者以及70 例正常人血清中SCC- Ag水平.鳞癌组SCC- Ag水平明显高于良性肿瘤组(P<0.01)、正常组(P<0.01)以及其他恶性肿瘤组(P<0.05).SCC- Ag可作为口腔鳞癌患者辅助诊断指标.

  16. Clinical Value of Combination of Multiple Tumor Markers in Diagno-sis of Lung Cancer%多种肿瘤标志物的联合检测诊断肺癌的临床价值

    Institute of Scientific and Technical Information of China (English)

    李远平; 彭华利; 龙永贵; 张帆

    2015-01-01

    Objective To investigate the application value of combination of multiple tumor markers [ carcinoembryonic antigen ( CEA) , a tumor-associated antigen ( CA-125) , squamous cell carcinoma associated antigen ( SCC-Ag) , neuron-specific enolase ( NSE) , cytokeratin 21-1 ( CY-FRA21-1 ) ] in the diagnosis of lung cancer.Methods A total of 165 patients with lung cancer and 104 patients with benign lung diseases who were treated at our hospital were included in the stud-y.Thirty healthy people who underwent physical checkup at the same time period served as con-trols.The serum levels of CEA, CA-125, SCC-Ag, NSE, CYFRA21-1 were detected in these sub-jects and analyzed after comparison between groups.Results Serum levels of CEA, CA-125, SCC-Ag, NSE, and CYFRA21-1 were significantly higher in lung cancer patients than in patients with benign lung diseases and healthy people and the difference was statistically significant ( P<0.05) .In single tumor marker detection, the sensitivity of CYFRA21-1 was highest, reaching 55.15%, and SCC-Ag had the highest specificity of 94.43%.The sensitivity of combined detection of the five tumor marker had a highest sensitivity of 91.24 % and a relatively lower specificity of 63.75 ( P<0.05 ) .In use of single tumor marker for detection of different types of lung cancer, SCC-Ag had a high value in diagnosis of small cell lung cancer, and high levels of CEA and CA125 indicated squamous cell carcinoma and adenocarcinoma.Combined detection of a number of tumor markers had a high positive value in diagnosis of these diseases.Conclusion Combined detection of multiple tumor markers has a higher value in diagnosis of lung cancer.The individual tumor marker is of significance in prediction of lung cancer of different pathological types.%目的:探讨癌胚抗原(carcino-embryonic antigen, CEA)、肿瘤相关抗原(CA-125)、鳞癌相关抗原(SCC-Ag)、神经元特异性烯醇化酶(Neuron-specific enolase, NSE

  17. 肿瘤标志物联合检测对非小细胞肺癌的诊断价值%Evaluation of combined measurement of serum cell keratin,tissue polypeptide antigen and squamous carcinoma associated antigen in the diagnosis of non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    曹永跃; 张铁; 王萍; 曹永彤

    2012-01-01

    目的:探讨血清细胞角质蛋白(CYFRA21-1)、组织多肽抗原(TPA)和鳞状细胞癌相关抗原(SCC-Ag)联合检测对非小细胞肺癌(NSCLC)的诊断价值,为临床早期诊断和治疗提供依据.方法:酶联免疫法检测51例非小细胞肺癌(经病理学确诊)、28例小细胞肺癌(经病理学确诊)、77例肺部良性疾病、57例正常人血清的CYFRA21-1、TPA和SCC-Ag的水平,并进行统计学分析.结果:对NSCLC的诊断,CYFRA21-1敏感性最高(72.88%),TPA特异性最高(82.09%),SCC-Ag检测对鳞癌与腺癌的鉴别诊断有统计学意义,三者联合检测NSCLC的敏感性为92.47%,特异性为94.78%,准确率为93.57%.结论:CYFRA21-1、SCC、TPA 3种肿瘤标记物联合检测NSCLC的敏感性及特异性显著高于各独立指标的检测,可以提高NSCLC的早期检出率.%Objective:To provide insights of the clinical value of combined measurement of the serum cell keratin (CYFRA21-1), tissue polypeptide antigen (TPA)and squamous carcinoma associated antigen (SCC-Ag)in the diagnosis of non-small cell lung cancer (NSCLC).Methods: The serum levels of CYFRA21-1 ,TPA and SCC-Ag in 51 NSCLC patients (pathological diagnosis) ,28 small cell lung cancer patients (pathological diagnosis), 77 benign pulmonary diseases and 57 healthy subjects were measured by enzyme linked immunosorbent assay (ELISA)and the data were analyzed statistically.ResultS:The sensitivity of the CYFRA21-1 to the diagnosis of NSCLC was 72.88%,and the specificity of the TPA was 82.09%.There was statistical differences between the SCC -Ag value of squamous cell carcinoma and that of adenocarcinoma.The sensitivity of CYFRA21-1,SCC and TPA combined measurement to NSCLC was 92.47% , the specificity was 94.78% , and the accuracy was 93.57%.Conclusion:The diagnosis level of CYFRA21 -1,SCC,TPA in the sensitivity and specificity are significantly higher than the independent testing.The combined measurement of CYFRA21-1, SCC-Ag and TPA can contribute to

  18. Application of serum tumor markers for predicting chemotherapy efficacy and prognosis in advanced esophageal squamous cell carcinoma%血清肿瘤标志物在晚期食管鳞状细胞癌疗效评价和预后判定中的作用

    Institute of Scientific and Technical Information of China (English)

    龚磊; 龚继芳; 张晓东

    2011-01-01

    Objective To assess the predictive and prognostic role of the alteration of serum carcinoembryonic antigen (CEA),squamous cell carcinoma antigen (SCC-Ag) and cytokeratin 19 fragment (CYFRA21-1) during chemotherapy in patients with advanced esophageal squamous cell carcinoma (ESCC).Methods The serum levels of CEA,CYFRA21-1 and SCC-Ag were measured in 50 patients with advanced ESCC at baseline and after first line chemotherapy.The data was correlated with objective efficacy and overall survival.To evaluate the role of tumor marker change in predicting response to therapy,receiver operating characteristic (ROC) curves were constructed.Results CEA,SCC-Ag and CYFRA21-1 increased higher than the cutoffs in 22.0 %,34.0 %,and 44.0 % of tested patients,respectively.Statistically significant correlations were observed between CYFRA21-1 and SCC responses and objective efficacy evaluated by RECIST criteria (Z =3.181,2.389; P = 0.001,0.017).Based on the ROG curve analysis,a post treatment 32 % and 38 % increase in serum concentration was used as cut-off level for defining CYFRA21-1 and SCC-Ag change,respectively.When the cut-off levels were used to predict chemotherapy efficacy,the accuracy for CYFRA21-1 and SCC-Ag were 76 % and 70 %.In the univariate survival analysis,a statistically significant prognostic impact on survival from the change of CYFRA21-1 and SCC-Ag was observed.Conclusion CYFRA21-1 and SCC-Ag are useful for diagnosis,predicting chemotherapy objective efficacy and prognosis in advanced ESCC.%目的 探讨血清肿瘤标志物在晚期食管鳞状细胞癌(简称:鳞癌)中的表达及其在化疗疗效评价和预后判断中的意义.方法 测定50例晚期食管鳞癌患者一线化疗前后血清标志物的水平.按照实体瘤的疗效评价标准(RECIST标准)评效.通过建立受试者工作特性曲线(ROC曲线)求最佳临界值.用Kaplan-Meier法行生存分析.结果 50例患者治疗前的血清肿瘤标志物阳性率以细胞角蛋白19片段(CYFRA

  19. 肺癌患者血清肿瘤标记物检测的临床意义及预后评估%Clinical significance and prognostic evaluation of serum tumor markers in patients with lung cancer

    Institute of Scientific and Technical Information of China (English)

    刘慧; 李永怀; 桂淑玉

    2014-01-01

    Objective To assess the diagnostic value of serum carcinoembryonic antigen ( CEA) , cytokera-tin 19 fragment (CYFRA21-1) and neuron specific enolase (NSE) in patients with lung cancer, and to investigate the relationship between the levels of three tumor markers and clinical characteristics, chemotherapeutic response, prognosis in patients with lung cancer. Methods The levels of serum CEA, CYFRA21-1and NSE were detected by Chemiluminescence ( CLIA) in 227 patients with lung cancer and 60 patients with benign pulmonary disease. At the same time, the clinical materials of the two groups were collected completely. PFS was taken from patients followed up until the disease progression. Statistical analysis was performed by using SPSS 17. 0 software. Results ① The levels of serum CEA, CYFRA21-1 and NSE in the lung cancer group were significantly higher than those in the be-nign pulmonary disease group (P<0. 01). ROC curves showed that the under-curve area of CEA, CYFRA21-1 and NSE was 0. 8, 0. 6 and 0. 8 respectively, each of which was more than 0. 5. The highest sensitivity of serum tumor markers in adencarcinoma, squamous cell carcinoma and small cell lung cancer was CEA, CYFRA21-1 and NSE re-spectively. Compared with each single item, the diagnostic sensitivity of the combined detection of the three tumor markers was greatly improved. ②The levels of CEA, CYFRA21-1 and NSE in patients at stageⅣwere significantly higher than those in patients at stagesⅠandⅡ(P<0. 05). The levels of the three tumor markers were all higher in patients with distant metastasis than those in patients without metastasis. ③ Before the treatment, the levels of CY-FRA21-1 and NSE in the PD group were significant higher than those in the DC group ( P<0. 05 ) . ④ The PFS of the patients with normal serum CYFRA21-1 and NSE before the treatment was longer than that of the patients with el-evated CYFRA21-1 and NSE (5. 16 ± 2. 56 vs 3. 03 ± 1. 41, 5. 12 ± 1. 54 vs 3. 90 ± 1. 86;P<0

  20. The evaluation of three tumor markers in diagnosis of lung cancer%3项肿瘤标志物联合检测在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    刘刚; 顾春瑜; 侯晓霞; 赵乔妹; 于勇

    2011-01-01

    目的:探讨联合检测血清癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)水平对肺癌的诊断价值.方法:用电化学发光法检测113例肺癌确诊患者、60例肺良性病变患者血清中CEA、CYFRA21-1、NSE水平.结果:肺癌组CEA、CYFRA21-1、NSE检测阳性率均明显高于肺良性病变组(P<0.01);CEA、CYFRA21-1NSE 3项联合检测肺癌的灵敏度为79.6%,明显高于单项检测肺癌的灵敏度(46.9%、46.9%和45.1%)和二项联合平行试验的诊断灵敏度(67.3%、70.8%和65.5%).结论:CEA、CYFRA21-1、NSE联合检测可明显提高对肺癌的诊断灵敏度,有利于肺癌的早期诊断.%To study the clinical significance of combination detection of carcino-embryonic antigen (CEA), CYFRA21-1 and neuron-specific enolase (NSE) in patients with lung cancer. Methods: One hundred and thirteen cases of lung tumor patients and 60 patients with benign lung diseases were enrolled. Their serum levels of CEA, CYFRA21-1 and NSE were detected by electro-chemi-luminescence assay. Results: The levels of CEA,CYFRA21-1 and NSE in lung cancer group were significantly higher than those of control group (P<0. 01). The sensitivity and specificity of measurement of three tumor markers in combination (79. 6%) were significantly higher than that of combination of two tumor markers(67. 3% ,70. 8% and 65. 5% in different combinations)and single tumor marker(46. 9% ,46. 9% and 45. 1%). Conclusion: The combined measurement of serum CEA,CYFRA21-1 and NSE significantly increased the sensitivity for diagnosis of lung cancer, and therefore it provides useful information for early diagnosis of patients with lung cancer.

  1. Isotopes——Development of a lmmunoradiometric Assay for Quantifying NSE in Human Serum

    Institute of Scientific and Technical Information of China (English)

    FANGJun-jian; YANQiang-feng

    2003-01-01

    Neuron-specific enolase(NSE) is a tumor marker for neuroblastoma and also one of the most sensitive and specific marker for small-cell lung cancer(SCLC) which has neuroendocrine properties with over-expressing of NSE. Quantitative assay for circulating NSE has clinic significance in monitoring of therapy and prognosis for the cancer.

  2. Anti-CDC25B autoantibody predicts poor prognosis in patients with advanced esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Dong Jun

    2010-09-01

    Full Text Available Abstract Background The oncogene CDC25B phosphatase plays an important role in cancer cell growth. We have recently reported that patients with esophageal squamous cell carcinoma (ESCC have significantly higher serum levels of CDC25B autoantibodies (CDC25B-Abs than both healthy individuals and patients with other types of cancer; however, the potential diagnostic or prognostic significance of CDC25B-Abs is not clear. The aim of this study is to evaluate the clinical significance of serum CDC25B-Abs in patients with ESCC. Methods CDC25B autoantibodies were measured in sera from both 134 patients with primary ESCC and 134 healthy controls using a reverse capture enzyme-linked immunosorbent assay (ELISA in which anti-CDC25B antibodies bound CDC25B antigen purified from Eca-109 ESCC tumor cells. The clinicopathologic significance of CDC25B serum autoantibodies was compared to that of the tumor markers carcinoembryonic antigen (CEA, squamous cell carcinoma antigen (SCC-Ag and cytokeratin 19 fragment antigen 21-1(CYFRA21-1. Results Higher levels of CDC25B autoantibodies were present in sera from patients with ESCC (A450 = 0.917, SD = 0.473 than in sera from healthy control subjects (A450 = 0.378, SD = 0.262, P 450 greater than the cut-off value of 0.725. Relatively few patients tested positive for the tumor markers CEA, SCC-Ag and CYFRA21-1 (13.4%, 17.2%, and 32.1%, respectively. A significantly higher number of patients with ESCC tested positive for a combination of CEA, SCC, CYFRA21-1 and CDC25B-Abs (64.2% than for a combination of CEA, SCC-Ag and CYFRA21-1 (41.0%, P P P = 0.001, log-rank. In the N1 subgroup, the cumulative five-year survival rate of CDC25B-seropositive patients was 13.6%, while that of CDC25B-seronegative patients was 54.5% (P = 0.040, log-rank. Conclusions Detection of serum CDC25B-Abs is superior to detection of the tumor markers CEA, SCC-Ag and CYFRA21-1 for diagnosis of ESCC, and CDC25B-Abs are a potential prognostic

  3. 血清肿瘤标志物联合检测对肺癌诊断的临床评价%Clinical values of combination measurement of tumor markers in diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    张长军

    2013-01-01

    Objective:To evaluate the clinical values of carcinoembryonic antigen(CEA),neuron specific enolase (NSE) ,squamous cell carcinoma antigen(SCC), carbohydrate antigen 125(CA125) and cytokeratin fragment 19 (CYFRA21 - 1) for diagnosis of lung cancer. Methods; The levels of serum CEA, NSE, SCC, CA125 and CY-FRA21 - 1 were measured in 103 lung cancer patients,85patients with benign lung diseases and 60 healthy persons. Results; The levels of CEA,NSE,SCC,CA125 and CYFRA21 - 1 were significantly higher in patients with lung cancer than those in patients with benign lung diseases and 60 healthy persons (P <0.05). The levels and positive rates of CEA and CA125 were significantly higher in patients with lung adenocarcinoma than those in patients with squamous cell carcinoma or small cell lung cancer. The levels and positive rates of SCC and CYFRA21 - 1 were significantly higher in patients with squamous cell carcinoma than those in patients with lung adenocarcinoma or small cell lung cancer. The level and positive rate of NSE were significantly higher in patients with small cell lung cancer than those in patients with squamous cell carcinoma or lung adenocarcinoma. The positive rate of single test of five tumor markers was less than 50%. The combination measurement of CEA + NSE, CEA + NSE + CYFRA21 - 1, CEA + NSE + CA125 + CYFRA21 - 1 were better, their positive rates were 65. 3% ,70.9% and 71. 8% in patients with lung cancer respectively. Conclusion; The levels and positive rates of serum CEA, NSE, SCC, CA125 and CY-FRA21 -1 were associated with pathology type of lung cancer. The combination measurement of serum CEA, NSE, SCC,CA125 and CYFRA21 -1 increased the sensitivity for diagnosis of lung cancer, the combination measurement of CEA,NSE and CYFRA21 - 1 was the best choice.%目的:评价癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、鳞状细胞癌抗原(SCC)、糖链抗原125(CA125)和细胞角蛋白19片断(CYFRA21-1)对肺癌诊断的临床价值.方法:

  4. Meiotic DNA joint molecule resolution depends on Nse5–Nse6 of the Smc5–Smc6 holocomplex

    Science.gov (United States)

    Wehrkamp-Richter, Sophie; Hyppa, Randy W.; Prudden, John; Smith, Gerald R.; Boddy, Michael N.

    2012-01-01

    Faithful chromosome segregation in meiosis is crucial to form viable, healthy offspring and in most species, it requires programmed recombination between homologous chromosomes. In fission yeast, meiotic recombination is initiated by Rec12 (Spo11 homolog) and generates single Holliday junction (HJ) intermediates, which are resolved by the Mus81–Eme1 endonuclease to generate crossovers and thereby allow proper chromosome segregation. Although Mus81 contains the active site for HJ resolution, the regulation of Mus81–Eme1 is unclear. In cells lacking Nse5–Nse6 of the Smc5–Smc6 genome stability complex, we observe persistent meiotic recombination intermediates (DNA joint molecules) resembling HJs that accumulate in mus81Δ cells. Elimination of Rec12 nearly completely rescues the meiotic defects of nse6Δ and mus81Δ single mutants and partially rescues nse6Δ mus81Δ double mutants, indicating that these factors act after DNA double-strand break formation. Likewise, expression of the bacterial HJ resolvase RusA partially rescues the defects of nse6Δ, mus81Δ and nse6Δ mus81Δ mitotic cells, as well as the meiotic defects of nse6Δ and mus81Δ cells. Partial rescue likely reflects the accumulation of structures other than HJs, such as hemicatenanes, and an additional role for Nse5–Nse6 most prominent during mitotic growth. Our results indicate a regulatory role for the Smc5–Smc6 complex in HJ resolution via Mus81–Eme1. PMID:22855558

  5. A Characterization of Projective Special Unitary Group U3(7 by nse

    Directory of Open Access Journals (Sweden)

    Shitian Liu

    2013-01-01

    Full Text Available Let G a group and ω(G be the set of element orders of G. Let k∈ω(G and let sk be the number of elements of order k in G. Let nse(G={sk∣k∈ω(G}. In Khatami et al. and Liu's works, L3(2 and L3(4 are uniquely determined by nse(G. In this paper, we prove that if G is a group such that nse(G = nse(U3(7, then G≅U3(7.

  6. 血清肿瘤标志物联合检测在肺癌诊断中的应用%Diagnostic value of serum tumor markers in lung cancer

    Institute of Scientific and Technical Information of China (English)

    许华斌

    2014-01-01

    Objective:To explore the diagnostic value of the combined detection of serum cancer antigen 125(CA125),carbohydrate antigen 242(CA242),carcinoembryonic antigen(CEA),cytokeratin 19 fragments(CYFRA21-1) and neuron specific enolase(NSE), tumor marker,in lung cancer. Methods:The biomarker levels of CA-125,CA242,CEA,CYFRA21-1 and NSE in 80 patients with lung cancer and 46 patients with benign lung disease were measured by the immunoluminometric assay. Results:The levels of CA-125, CA242,CEA,CYFRA21-1 and NSE in the patients with lung cancer were significantly higher than those in the patients with benign lung disease(PCEA>CA125>NSE>CA242;在腺癌中CYFRA21-1灵敏度最高(50.00%),鳞癌中CYFRA21-1灵敏度最高(48.94%),小细胞肺癌中CA125和NSE灵敏度最高(48.15%)。 CA125、CA242、CEA、CYFRA21-1和NSE的ROC曲线下面积分别为0.669±0.095、0.608±0.097、0.682±0.096、0.714±0.090、0.660±0.096。在肺癌诊断中CA125+CA242+CEA+CYFRA21-1+ NSE组合灵敏度最高(70.00%),特异性也较好(84.78%);腺癌诊断中联合检测灵敏度与单项CYFRA21-1检测灵敏度相同(50.00%),但联合检测的约登指数大于单项CYFRA21-1;鳞癌诊断中CA125+CA242+CEA+CYFRA21-1组合灵敏度最高(72.34%),小细胞肺癌中CA125+CA242+CEA+CYFRA21-1+NSE组合灵敏度最高(88.89%)。结论:CA125、CA242、CEA、CYFRA21-1和NSE对肺癌的诊断均有一定意义,但单一指标诊断的灵敏度和特异性都有一定局限,联合检测可有效提高肺癌诊断的灵敏度和特异性。

  7. Plasma S100 beta and NSE levels and progression in multiple sclerosis

    NARCIS (Netherlands)

    Koch, Marcus; Mostert, Jop; Heersema, Dorothea; Teelken, Albert; De Keyser, Jacques

    2007-01-01

    Plasma levels of the glial cell marker S100 beta and the neuronal marker neuron-specific enolase (NSE) are elevated in various conditions of central nervous system damage. In this study we investigated whether plasma levels of S 1000 and NSE are related to disease progression in multiple sclerosis (

  8. Plasma S100 beta and NSE levels and progression in multiple sclerosis

    NARCIS (Netherlands)

    Koch, Marcus; Mostert, Jop; Heersema, Dorothea; Teelken, Albert; De Keyser, Jacques

    2007-01-01

    Plasma levels of the glial cell marker S100 beta and the neuronal marker neuron-specific enolase (NSE) are elevated in various conditions of central nervous system damage. In this study we investigated whether plasma levels of S 1000 and NSE are related to disease progression in multiple sclerosis (

  9. Immunoreactive neuron-specific enolase (NSE) is expressed in testicular carcinoma-in-situ

    DEFF Research Database (Denmark)

    Kang, J L; Rajpert-De Meyts, E; Skakkebaek, N E

    1996-01-01

    -seminomas, and a mixed germ cell tumour. As the co-existence of high NSE production and gene amplification of N-myc has been reported in some tumours, including germ cell tumours, the expression of the protein product of N-myc was also examined in this study, but only sporadic cases showed N-myc staining. These results......Neuron-specific enolase (NSE) is a well-known marker of tumours that have neuroendocrine origin. High levels of NSE have also been described in various types of testicular germ cell neoplasms, particularly in seminomas. To evaluate the presence of NSE in testicular carcinoma-in situ (CIS......), a preinvasive stage of testicular germ cell tumours, a panel of CIS tissue specimens was examined. Fifteen of 18 (83 per cent) CIS samples showed immunohistochemical staining with anti-NSE monoclonal antibody. Immunoreactivity has also been found in overt testicular germ cell tumours, including seminomas, non...

  10. Detection of serum p53 antibodies in patients with esophageal squamous cell carcinoma: correlation with clinicopathologic features and tumor markers.

    Science.gov (United States)

    Shimada, H; Nakajima, K; Ochiai, T; Koide, Y; Okazumi, S I; Matsubara, H; Takeda, A; Miyazawa, Y; Arima, M; Isono, K

    1998-01-01

    The significance of serum p53-Abs in patients with esophageal squamous cell carcinoma was determined. Examination of clinicopathological features and assessment of tumor marker sensitivities of carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag) and CYFRA21-1 were performed. Thirty-three (58%) of 57 patients were positive for serum p53-Abs, however, no relation with cancer progression existed. Fourteen of the 33 sero-positive patients revealed normal levels of all tumor markers tested. Thus, serum p53-Abs appears to be a useful marker for the detection of esophageal squamous cell carcinoma.

  11. CA125、CYFRA21-1、HPV 联合检测 TCT 诊断宫颈癌的临床价值%Clinical value of CA125,CYFRA21-1,HPV detection combined with TCT in diagnosis of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    黄伟强; 杨艳梅

    2015-01-01

    Objective To investigate the value of cytokeratin fragment antigen 21‐1(CYFRA21‐1) ,carbohydrate antigen‐125 (CA125) ,human papillomavirus (HPV ) detection with Thinprep cytologic test (TCT ) in the diagnosis of cervical cancer . Methods 70 patients with cervical cancer ,60 patients with benign cervical diseases and 70 healthy people were selected .CYFRA21‐1 ,CA125 ,HPV detection and TCT results were analyzed .Results The positive rates of HPV ,TCT ,CA125 and CYFRA21‐1 in cervical cancer group were significantly higher than those in cervical benign lesion group and the healthy group ,the difference were statistically significant (P TSGF>CEA>SIL‐2R .The specificity was CYFRA21‐1> TCT>CA125> HPV .In HPV ,TCT ,CA125 and CYFRA21‐1parallel diagnostic test ,the sensitivity was up to 100 .00% ,and in series diagnostic test ,the specificity was up to 98 .3% .Conclu‐sion HPV ,CA125 ,CYFRA21‐1 detection combined with TCT in diagnosis of cervical cancer has strong reliability ,it is worth u‐sing w idely in clinic .%目的:探讨细胞角蛋白21‐1(CYFRA21‐1)、糖类抗原‐125(CA125)、人乳头瘤病毒(HPV)检测联合新柏氏液基细胞学检查(TCT)在宫颈癌诊断的临床价值。方法选取宫颈癌患者70例,宫颈良性疾病60例和同期检查的健康者70例作为健康组,对3组人群CYFRA21‐1、CA125、HPV检测与TCT结果进行分析。结果 HPV、TCT、CA125与CYFRA21‐1在宫颈癌患者中检出阳性率明显高于宫颈良性病变组和健康组,差异有统计学意义(P<0.01);HPV、TCT、CA125与CYFRA21‐1诊断宫颈癌的敏感度从大到小依次为 TCT、CYFRA21‐1、CA125、HPV ,特异度从高到低分别是 CYFRA21‐1、TCT、CA125、HPV。 HPV、TCT、CA125与CYFRA21‐1进行并联诊断试验中敏感度高达100.00%,在串联诊断试验中特异度高达98.3%。结论 HPV、CA125、CYFRA21‐1检测与TCT联合诊断宫颈癌具有较强的可靠性,临床上值得推广使用。

  12. Value of Combined Detection of Tumor Markers Such as CEA and CA125 in the Diagnosis of Lung Cancer%CEA、CA125等肿瘤标志物联合检测在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    李林; 王迪进; 万轲; 徐国辉

    2015-01-01

    Objective To study the value of serum carcinoembryonic antigen ( CEA ) , carbohydrate antigen 125 (CA125),cytokeratin 19 fragments (CYFRA21-1),neuron-specific enolase (NSE) and squamous cell carcinoma antigen (SCC) in the diagnosis of lung cancer.Methods 102 cases of lung cancer patients,65 cases of lung benign diseases and 80 cases of healthy patients were randomly selected.5ml peripheral venous blood was extracted from every research object and the SCC,NSE, CA125,CYFRA21-1 and CEA were detected.Results The positive rates of SCC,NSE,CA125,CYFRA21-1 and CEA of patients in lung cancer group were significantly higher than those in other 2 groups(P<0.05).The sensitivity of 5 tumor markers com-bined detection in the diagnosis of lung cancer (91.18%) was significantly higher than each one of CEA,CA125,CYFRA21-1, NSE and SCC(40.20%,42.16%,50.00%,36.27%,20.59%)(P<0.05).Conclusion CEA,CA125,CYFRA21-1,NSE and SCC have certain value in the clinical diagnosis and classification of lung cancer,and the combined detection of the 5 tumor mark-ers can significantly increase the sensitivity.%目的:探讨癌胚抗原(CEA)、糖类抗原125(CA125)、细胞角蛋白19片段21-1(CYFRA21-1)、神经元特异性烯醇化酶(NSE)以及鳞状上皮细胞癌相关抗原(SCC)5种肿瘤标志物联合检测在肺癌诊断中的价值。方法随机选择肺癌患者102例、肺良性疾病65例以及同期体检健康者80例作为研究对象。所有研究对象均于空腹抽取5 ml外周静脉血,检测CEA、CA125、CYFRA21-1、NSE和SCC浓度。结果肺癌组患者CEA、CA125、CYFRA21-1、NSE及SCC检测阳性率均显著高于肺良性疾病患者、健康组(P<0.05);5种肿瘤标志物联合检测诊断肺癌的敏感度(91.18%)显著高于CEA、CA125、CYFRA21-1、NSE及SCC单项检测敏感度(42.20%、42.16%、50.00%、36.27%、20.59%)(P<0.05)。结论CEA、CA125、CYFRA21-1、NSE及SCC在肺

  13. Analysis of Application of Serum Tumor Markers in the Diagnosis of Esophageal Carcinoma%血清肿瘤标志物在食管癌诊断中的运用分析

    Institute of Scientific and Technical Information of China (English)

    吴正吉; 张渝成; 徐维凤

    2014-01-01

    Objective Objective To study the application of serum tumor markers neuron-specific enolase ( NSE) ,cytokera-tin 19 fragment antigen(CYFRA21-1)and carcinoembryonic antigen (CEA)in the diagnosis of esophageal cancer .Methods Ser-um level of NSE ,CYFRA21-1 and CEA in 75 cases of normal healthy subjects ( control group ) and 70 patients with esophageal cancer(experimental group)were detected by Roche electrochemiluminescence immune system .Results Serum level of NSE, CYFRA21-1,CEA in the study group was significantly higher than that of the control group (P<0.05);test results of the 3 tumor markers showed that NSE ,CYFRA21-1,CEA for the diagnosis of esophageal cancer had a higher specificity but low sensitivity . Combined detection of the 3 tumor markers for the diagnosis of esophageal cancer had a high sensitivity and specificity of 81.9%(59/72)and 83.3%(60/72).Conclusion NSE,CYFRA21-1 and CEA can be used as an auxiliary index for the diagnosis of e-sophageal cancer ,it can effectively improve the diagnostic sensitivity ,and it is worthy of clinical application .%目的:探讨血清肿瘤标志物神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段抗原(CYFRA21-1)和癌胚抗原( CEA)在食管癌诊断中的运用。方法对75例正常的健康受试者(对照组)和70例食管癌患者(研究组)使用罗氏电化学发光免疫系统检测其血清NSE、CYFRA21-1、CEA水平。结果研究组NSE、CYFRA21-1、CEA血清含量均明显高于对照组(P<0.05);3种肿瘤标志物的检测结果显示,NSE、CYFRA21-1、CEA在食管癌的诊断中均有较高特异性,但敏感性较低。3种标志物的联合检测对于食管癌的诊断有较高敏感性以及特异性,分别是81.9%(59/72)和83.3%(60/72)。结论 NSE、CYFRA21-1、CEA可以作为食管癌诊断的辅助指标,能有效提高食管癌诊断的敏感性,值得在临床推广应用。

  14. CLINICOPATHOLOGICAL SIGNIFICANCE OF PLAP, NSE AND WT1 DETECTION IN OVARIAN DYSGERMINOMA

    Institute of Scientific and Technical Information of China (English)

    赵涌; 李圆圆; 徐元浩

    2003-01-01

    Objective: To evaluate the expression of placental alkaline phosphatase (PLAP), neuron-specific enolase (NSE), prolactin (PRL) and Wilms' tumor gene (WT1) protein in ovarian dysgerminoma and its Clinico- pathological significance. Methods: Clinicopathological data were retrospectively reviewed in a total of 31 patients with pure dysgerminoma who were treated at College Clinical Medicin, Chongqing Medical University from January 1983 to October 2002. Immunohistological staining for PLAP, NSE, PRL and WT1 was performed in all 31 tumor tissues. Results: The age of the patients ranged from 12 to 42 years old (average 25 yrs). According to the clinical staging, eighteen patients (58.1%) had stage I disease, 5 (16.1%) had stage II, 6(19.4%) had stage III, and 2 (6.4%) had stage IV disease. Of the 31 cases, the positive expression rates of PLAP, NSE, PRL and WT1 were 100% (31/31), 70.9%(22/31), 3.2%(1/31) and 12.9(4/31) respectively. There was a significant relation between NSE expression and clinical stages (P<0.05) and 5 years survival rate (P<0.05). The positive expression rate of WT1 was significantly related to histological types (P<0.05). Conclusion: Dysgerminomas in earlier stages (stage I/II) are associated with a favorable prognosis. NSE positive expression is closely related with advanced tumor stage (stage III/IV). PLAP and NSE can be considered as important markers of dysgerminoma tissues, and the tumor tissues are the main resources of serum PLAP and NSE. WT1 expression correlates with poorer differentiation of dysgerminoma. The importance of PRL in dygerminoma was not certified in this study, and remains to be defined.

  15. Instrument developments and recent scientific highlights at the J-NSE

    Science.gov (United States)

    Ivanova, Oxana; Pasini, Stefano; Monkenbusch, Michael; Holderer, Olaf

    2017-06-01

    The J-NSE neutron spin echo spectrometer faces now 10 years of successful user operation at the FRM II research reactor at the Heinz Maier-Leibnitz Zentrum (MLZ). We present scientific highlights and instrumental developments of the last decade, for example the development of grazing incidence neutron spin echo spectroscopy (GINSES) at the J-NSE and investigations of the dynamics at solid-liquid interfaces with this new option. Polymers in confinement have been a prominent topic, as well as the internal dynamics of proteins. The scientific questions also triggered instrumental developments such as a new polarizer and a new neutron guide concept. Finally, the future of the J-NSE will be addressed with a short presentation of the current upgrade program with superconducting main coils with reduced intrinsic field integral inhomogeneity.

  16. 食管癌淋巴结转移与早期食管癌治疗前后血清肿瘤标志物的比较%DIFFERENT CONCENTRATION OF SERUM TUMOR MARKERS IN BEFORE AND AFTER TREATMENT OF VARIANT STAGE AND PERIOD OF ESOPHAGEAL CANCER

    Institute of Scientific and Technical Information of China (English)

    江奇峰; 郭骏; 张菲; 周琴笑

    2014-01-01

    目的 探讨食管癌淋巴结转移患者与早期食管癌患者在接受治疗前后血清中细胞角蛋白19(CY-FRA21-1)、癌胚抗原(CEA)、鳞状细胞癌抗原(SSCA)和神经元特异性烯醇化酶(NSE)肿瘤标志物的含量差异.方法 采用罗氏电化学发光免疫分析法(ECLIA)检测35例食管癌伴有淋巴结转移患者术前和40例早期食管癌患者血清中CYFRA21-1、CEA、SCCA和NSE含量.结果 伴有淋巴结转移食管癌患者血清CYFRA21-1、CEA、SCCA和NSE在治疗前后均明显高于早期食管癌,差异具有统计学意义(P<0.05).结论 CYFRA21-1、CEA、SCCA和NSE等血清肿瘤标志物可作为食管癌预后的重要辅助指标,具有一定临床应用价值.

  17. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons.

    Directory of Open Access Journals (Sweden)

    Mandy Ka Man Kam

    Full Text Available To establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer, which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult.

  18. Serum levels of S100B and NSE proteins in Alzheimer's disease patients

    Directory of Open Access Journals (Sweden)

    Souza Diogo O

    2010-01-01

    Full Text Available Abstract Background Alzheimer's disease is the most common dementia in the elderly, and the potential of peripheral biochemical markers as complementary tools in the neuropsychiatric evaluation of these patients has claimed further attention. Methods We evaluated serum levels of S100B and neuron-specific enolase (NSE in 54 mild, moderate and severe Alzheimer's disease (AD patients and in 66 community-dwelling elderly. AD patients met the probable NINCDS-ADRDA criteria. Severity of dementia was ascertained by the Clinical Dementia Rating (CDR scale, cognitive function by the Mini Mental State Examination (MMSE, and neuroimage findings with magnetic resonance imaging. Serum was obtained from all individuals and frozen at -70°C until analysis. Results By comparing both groups, serum S100B levels were lower in AD group, while serum NSE levels were the same both groups. In AD patients, S100B levels were positively correlated with CDR scores (rho = 0.269; p = 0.049 and negatively correlated with MMSE scores (rho = -0.33; P = 0.048. NSE levels decreased in AD patients with higher levels of brain atrophy. Conclusions The findings suggest that serum levels of S100B may be a marker for brain functional condition and serum NSE levels may be a marker for morphological status in AD.

  19. [Distribution of nervous system-specific enolase (NSE) and S-100 protein in the gastrointestinal tract of congenital aganglionosis rat].

    Science.gov (United States)

    Horisawa, M; Isiguro, Y; Ito, T; Kato, K

    1985-02-01

    Immunobiochemical and immunohistochemical studies were performed to find out changes in neural and glial components of the intestine in congenital aganglionosis rats. Nervous system-specific enolase (NSE) was used as a marker of the neural component. S-100 protein was used as a marker of the glial component. NSE and S-100 protein were quantitated by highly sensitive immunoassay. And distribution of NSE and S-100 protein of the intestine was studied by immunohistochemical methods in fetal rats of various gestational age. The results of our studies were followed; Significant decrease in NSE and S-100 protein was found in the aganglionic segment. Although there was no decrease in NSE, significant decrease in S-100 protein was found in the ganglionic segment just proximal to the aganglionic narrow segment. In the studies using rat embryos, NSE positive cells and S-100 protein positive cells were identified in the more caudal intestine with increasing gestational age. In addition, NSE positive cells appeared in the intestine few days earlier than S-100 protein positive cells did. In conclusion, our studies disclosed that there was abnormality of the glial component as well as the neural component in the intestine of Hirschsprung's disease.

  20. 血清肿瘤标志物检测在肺癌诊断中价值

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    Objective To investigate the serum carcinoembryonic antigen(CEA),cytokeratin 19 fragment(CYFRA 21-1),neuron specific enolase(NSE),squamous cell carcinoma antigen(SCC)concentration in lung cancer diagnosis, pathological typing and clinical staging significance. Methods Select 125 cases of patients with lung cancer histology and cytology confirmed as the observation group,125 patients with benign pulmonary disease patients as control group,two groups of patients with serum CEA concentration in 21-1,CYFRA,NSE,SCC were measured. Results ①The observation group of four tumor markers in serum concentration was significantly higher than that of the control group(PCYFRA21-1 SCC,serum SCLC NSE concentration was highest(P<0.05). ③The serum levels of four tumor markers in lung cancer TNM concentration is higher in later clinical stages was higher(P<0.05). ④The joint detection in diagnosis of lung cancer was significantly higher than that of the sensitivity and accuracy of the single detection(P<0.01),the specificity of combined detection of CEA,CYFRA21-1 decreased,and the contrast(P<0.01). Conclusion Pathological detection of SCC and CEA,CYFRA,21-1,NSE in serum in lung cancer diagnosis,classification,prognosis has good clinical value and is worth for clinical application.%  目的探讨血清中癌胚抗原(CEA)、可溶性细胞角蛋白19片段(CYFRA 21-1)、神经元特异性烯醇化酶(NSE)、鳞状细胞癌相关抗原(SCC)浓度对肺癌的诊断、病理分型及临床分期的意义。方法选择经组织学和细胞学确诊的125例肺癌患者作为观察组,125例肺部良性病变患者作为对照组,分别测定两组患者血清中CEA、CYFRA 21-1、NSE、SCC的浓度。结果(1)观察组血清中四项肿瘤标志物浓度明显高于对照组(P<0.01)。(2)腺癌血清CEA浓度最高,鳞癌血清CYFRA

  1. Prediction of lung cancer based on serum biomarkers by gene expression programming methods.

    Science.gov (United States)

    Yu, Zhuang; Chen, Xiao-Zheng; Cui, Lian-Hua; Si, Hong-Zong; Lu, Hai-Jiao; Liu, Shi-Hai

    2014-01-01

    In diagnosis of lung cancer, rapid distinction between small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) tumors is very important. Serum markers, including lactate dehydrogenase (LDH), C-reactive protein (CRP), carcino-embryonic antigen (CEA), neurone specific enolase (NSE) and Cyfra21-1, are reported to reflect lung cancer characteristics. In this study classification of lung tumors was made based on biomarkers (measured in 120 NSCLC and 60 SCLC patients) by setting up optimal biomarker joint models with a powerful computerized tool - gene expression programming (GEP). GEP is a learning algorithm that combines the advantages of genetic programming (GP) and genetic algorithms (GA). It specifically focuses on relationships between variables in sets of data and then builds models to explain these relationships, and has been successfully used in formula finding and function mining. As a basis for defining a GEP environment for SCLC and NSCLC prediction, three explicit predictive models were constructed. CEA and NSE are frequently- used lung cancer markers in clinical trials, CRP, LDH and Cyfra21-1 have significant meaning in lung cancer, basis on CEA and NSE we set up three GEP models-GEP 1(CEA, NSE, Cyfra21-1), GEP2 (CEA, NSE, LDH), GEP3 (CEA, NSE, CRP). The best classification result of GEP gained when CEA, NSE and Cyfra21-1 were combined: 128 of 135 subjects in the training set and 40 of 45 subjects in the test set were classified correctly, the accuracy rate is 94.8% in training set; on collection of samples for testing, the accuracy rate is 88.9%. With GEP2, the accuracy was significantly decreased by 1.5% and 6.6% in training set and test set, in GEP3 was 0.82% and 4.45% respectively. Serum Cyfra21-1 is a useful and sensitive serum biomarker in discriminating between NSCLC and SCLC. GEP modeling is a promising and excellent tool in diagnosis of lung cancer.

  2. The diagnostic significance of single or combination lung cancer-related serum biomarkers in high risk lung cancer patients%单独与联合检测四项肺癌血清肿瘤标志物在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    倪军; 郭子健; 张力

    2016-01-01

    Objective The aim of this study was to assess the clinical value of pro-gastrin releasing peptide (ProGRP) , squamous cell carcinoma antigen (SCC-Ag), cytokeratin 19 fragment antigen 21-1 (Cyfra21-1) and carcino-embryonic antigen (CEA) in the diagnosis and clinical stage of lung cancer in Chinese patients.Methods Patients with lung cancer and benign lesions confirmed by pathology were enrolled in Peking Union Medical College Hospital from January 2013 to October 2014.The serum levels of four tumor markers (ProGRP, SCC-Ag, Cyfra21-1 and CEA) were measured using immunoassays before treatment.The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and the areas under the receiver operating characteristic curve (AUCROC) of these four tumor biomarkers were analyzed for the diagnosis of lung cancer.Results A total of 134 patients were finally analyzed, including 73 patients with lung cancer and the other 61 patients with benign lung disease.The diagnostic sensitivity of serum Cyfra21-1 to lung cancer was 67.1%, the specificity 45.1%, the AUCROC 0.658.The diagnostic sensitivity of the panel including ProGPR, Cyfra21-1 and CEA to lung cancer was 75.3% , the specificity 57.4% , the AUCROC 0.702.In the lung cancer group, the AUCROC of ProGRP over 65 ng/L to diagnose small cell lung cancer was 0.954;the AUCROC of SCC-Ag over 1.5 μg/L to diagnose squamous cell lung cancer was 0.788;the AUCROC of Cyfra21-1 to diagnose non-squamous-non-small-cell lung cancer was 0.716.In small cell lung cancer patients, the level of ProGRP in limited-disease small cell lung cancer (LD-SCLC) were significantly higher than that in extensive-disease small cell lung cancer (ED-SCLC) (P =0.005).Conclusion This panel of serum tumor markers including ProGRP, Cyfra21-1 and CEA improves the diagnostic specificity and sensitivity in patients with high-risk lung cancer.The serum CEA level of advanced lung cancer patients is significantly increased.The high

  3. The Power (Law) of Indian Markets: Analysing NSE and BSE trading statistics

    CERN Document Server

    Sinha, S; Sinha, Sitabhra; Pan, Raj Kumar

    2006-01-01

    The nature of fluctuations in the Indian financial market is analyzed in this paper. We have looked at the price returns of individual stocks, with tick-by-tick data from the National Stock Exchange (NSE) and daily closing price data from both NSE and the Bombay Stock Exchange (BSE), the two largest exchanges in India. We find that the price returns in Indian markets follow a fat-tailed cumulative distribution, consistent with a power law having exponent $\\alpha \\sim 3$, similar to that observed in developed markets. However, the distributions of trading volume and the number of trades have a different nature than that seen in the New York Stock Exchange (NYSE). Further, the price movement of different stocks are highly correlated in Indian markets.

  4. The Power (Law) of Indian Markets: Analysing NSE and BSE Trading Statistics

    Science.gov (United States)

    Sinha, Sitabhra; Pan, Raj Kumar

    The nature of fluctuations in the Indian financial market is analyzed in this paper. We have looked at the price returns of individual stocks, with tick-by-tick data from the National Stock Exchange (NSE) and daily closing price data from both NSE and the Bombay Stock Exchange (BSE), the two largest exchanges in India. We find that the price returns in Indian markets follow a fat-tailed cumulative distribution, consistent with a power law having exponent α ˜ 3, similar to that observed in developed markets. However, the distributions of trading volume and the number of trades have a different nature than that seen in the New York Stock Exchange (NYSE). Further, the price movement of different stocks are highly correlated in Indian markets.

  5. 联合检测肿瘤标志物在肺癌诊断中的临床价值%Diagnostic value of combined detection of serum tumor markers for lung cancer

    Institute of Scientific and Technical Information of China (English)

    李艳平; 王群; 赵姿红; 周珊

    2013-01-01

    目的 探讨肿瘤标志物CEA、CA125、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段抗原(CYFRA21-1)联合检测在肺癌中的诊断价值.方法 研究对象为2010年10月至2012年3月的确诊肺癌住院患者138例(男82例,女56例,平均年龄58.6岁),肺良性病变住院患者96例(男56例,女40例,平均年龄51.3岁);健康体格检查者45名(对照组,男30名,女15名,平均年龄43.9岁).用电化学发光法检测血清CEA、CA125、NSE和CYFRA21-1水平,各指标的组间比较采用单因素方差分析及q检验.按病理类型分组,对鳞状细胞癌[简称鳞癌(66例)]、腺癌(52例)和小细胞肺癌(20例)的肿瘤标志物水平进行比较,计算单项及联合检测对各类型肺癌的诊断效能.结果 肺癌组血清CEA、CA125、NSE、CYFRA21-1水平均高于肺部良性病变组和健康对照组[CEA:(19.99±30.99)、(10.78± 19.77)、(3.25±3.42) μg/L,CA125:(79.70±95.98)、(44.96±44.97)、(20.66±7.13) μg/L,NSE:(35.23±40.22)、(15.31±8.42)、(13.30± 5.65) μg/L,CYFRA21-1:(18.07±43.71)、(8.30±8.83)、(3.13±1.60) μg/L;F=4.481、5.436、4.776和6.002,均P<0.05].CEA在腺癌中水平较鳞癌和小细胞肺癌高(F=4.932,P<0.05),NSE在小细胞肺癌中水平较高(F=5.119,P<0.05),CYFRA21-1在鳞癌中水平较高(F=5.378,P<0.05).单项肿瘤标志物在肺癌诊断中灵敏度:鳞癌中CYFRA21-1灵敏度最高(78.8%,52/66),腺癌中CEA灵敏度最高(57.7%,30/52),而在小细胞肺癌中NSE灵敏度最高(75.0%,15/20).在联合检测中CEA+CYFRA21-1+ NSE组合对鳞癌诊断灵敏度最高(89.4%,59/66),腺癌诊断中CEA+CA125+NSE组合灵敏度最高(78.8%,41/52),小细胞肺癌中CEA+CYFRA21-1+NSE组合灵敏度最高(80.0%,16/20).结论 CEA、CA125、NSE、CYFRA21-1联合检测对肺癌的诊断有一定的临床价值,不同病理类型肺癌4种肿瘤标志物表达有所不同.%Objective To investigate the diagnostic value of combined detection of serum tumor markers

  6. Predictive and prognostic value of preoperative serum tumor markers is EGFR mutation-specific in resectable non-small-cell lung cancer

    Science.gov (United States)

    Jiang, Richeng; Wang, Xinyue; Li, Kai

    2016-01-01

    Background The predictive and prognostic value of carcinoembryonic antigen (CEA), cytokeratin-19 fragments (Cyfra21-1), squamous cell carcinoma antigen (SCCA) and neuron-specific enolase (NSE) has been investigated in non-small-cell lung cancer (NSCLC) patients. However, few studies have directly focused on the association between these markers and epidermal growth factor receptor (EGFR) mutation status or mutation subtypes. Patients and methods We retrospectively analyzed 1016 patients with stage I-IIIA NSCLC who underwent complete resection between 2008 and 2012. Correlations between serum tumor marker levels and EGFR mutations and survival parameters were analyzed and prognostic factors were identified. Results Cyfra21-1 levels (P = 0.032 for disease-free survival [DFS]; P < 0.001 for overall survival [OS]) and clinical stage were identified as independent predictive and prognostic factors in EGFR-mutated adenocarcinoma patients. CEA levels (P < 0.001 for DFS; P = 0.002 for OS) and clinical stage were independently predictive and prognostic in EGFR wild-type adenocarcinoma patients. Further stratification analysis revealed that in EGFR exon 19 deletion adenocarcinomas, elevated Cyfra21-1 was an independent prognostic factor (P = 0.002). Within the Leu858Arg substitution subgroup, increased CEA (P = 0.005) and clinical stage were predictive factors of DFS, while elevated CEA (P = 0.005) and Cyfra21-1 (P = 0.027) were independent prognostic factors. Conclusion Cyfra21-1 and CEA exhibit different predictive and prognostic values between EGFR-mutated and wild-type adenocarcinomas, as well as between EGFR mutation subtypes. The prognostic impact of preoperative serum tumor markers should be evaluated together with EGFR mutation status. PMID:27072585

  7. Decomposition of the Mean Squared Error and NSE Performance Criteria: Implications for Improving Hydrological Modelling

    Science.gov (United States)

    Gupta, Hoshin V.; Kling, Harald; Yilmaz, Koray K.; Martinez-Baquero, Guillermo F.

    2009-01-01

    The mean squared error (MSE) and the related normalization, the Nash-Sutcliffe efficiency (NSE), are the two criteria most widely used for calibration and evaluation of hydrological models with observed data. Here, we present a diagnostically interesting decomposition of NSE (and hence MSE), which facilitates analysis of the relative importance of its different components in the context of hydrological modelling, and show how model calibration problems can arise due to interactions among these components. The analysis is illustrated by calibrating a simple conceptual precipitation-runoff model to daily data for a number of Austrian basins having a broad range of hydro-meteorological characteristics. Evaluation of the results clearly demonstrates the problems that can be associated with any calibration based on the NSE (or MSE) criterion. While we propose and test an alternative criterion that can help to reduce model calibration problems, the primary purpose of this study is not to present an improved measure of model performance. Instead, we seek to show that there are systematic problems inherent with any optimization based on formulations related to the MSE. The analysis and results have implications to the manner in which we calibrate and evaluate environmental models; we discuss these and suggest possible ways forward that may move us towards an improved and diagnostically meaningful approach to model performance evaluation and identification.

  8. 四种肿瘤标志物对肺癌的诊断价值研究%Diagnostic value of four kinds of tumor marker for lung cancer

    Institute of Scientific and Technical Information of China (English)

    庞华春; 傅健; 李双佐

    2015-01-01

    目的::探讨不同水平细胞角蛋白片段抗原21-1( CYFRA21-1)、糖类抗原125( CA125)、癌胚抗原( CEA)和神经元特异性烯醇化酶( NSE)在肺癌诊断中的价值。方法:采用电化学发光免疫法检测54例肺癌患者、60例健康体检者和60例肺部良性疾病患者的 CYFRA21-1、CAl25、CEA和NSE水平,对不同组别和不同肺癌分型患者之间的肿瘤标志物水平进行统计学分析。结果:肺癌组 CYFRA21-1、CAl25、CEA、NSE水平均高于良性疾病组和健康组( P<0.05);小细胞癌患者NSE水平显著高于鳞癌、腺癌患者(P<0.05);肺癌组各肿瘤标志物联合检测阳性例数显著高于各肿瘤标志物单个检测阳性例数(P<0.05);联合检测敏感性、准确性均高于肿瘤标志物单项检测(P<0.05)。结论:CYFRA21-1、CAl25、CEA和NSE可作为肺癌患者早期诊断的辅助指标,四者联合检测可提高诊断的准确性和敏感性。%Objective:To investigate the value of cytokeratin fragment antigen 21-1 ( CYFRA21-1 ) , carbohydrate antigen 125 ( CAl25 ) , carcinoembryonic antigen ( CEA ) and neuron specific enolase ( NSE ) of different levels in the diagnosis of lung cancer. Methods:The CYFRA21-1, CAl25, CEA and NSE levels in 54 patients with lung cancer, 60 healthy subjects and 60 patients with benign lung disease were determined by the Electrochemiluminescence immunoassay. The tumor markers between different groups and patients with different types of lung cancer were statistically analyzed. Results:The CYFRA21-1, CAl25, CEA, and NSE levels in the lung cancer group were higher than those in the benign disease group and healthy group (P<0.05). The NSE level in patients with small cell carcinoma was significantly higher than that in patients with squamous cell carcinoma and adenocarcinoma (P<0.05). The number of positive cases examined by tumor marker combined examination were higher than that determined by tumor marker single examination in the

  9. Clinical significance of joint detection for serum tumor markers for diagnosis of lung cancer%血清肿瘤标志物联合检测在肺癌诊断中的临床意义

    Institute of Scientific and Technical Information of China (English)

    李媛媛

    2015-01-01

    Objective To investigate the clinical application value of joint detection for serum tumor markers, such as carcino embryonie antigen (CEA), cytokeratins 19 fragment (CYFRA21-1), neuron specific enolase (NSE), and squamous cell carcinoma antigen (SCCA) for diagnosis of lung cancer.Methods There were 68 patients with lung cancer as the lung cancer group, and another 65 healthy people as the control group. Microparticle chemiluminescence was applied for content detection of CEA, CYFRA21-1, NSE, and SCCA. Comparisons were made on sensitivity, specificity and accuracy between single and joint detection of tumor markers.Results The lung cancer group had much higher contents of CEA, CYFRA21-1, NSE, and SCCA than the control group (P Conclusion Detection of serum CEA, CYFRA21-1, NSE, and SCCA has clinical application value in diagnosis of lung cancer. Joint detection can improve sensitivity and accuracy of diagnosis with good specificity and it is helpful for improving detection rate of lung cancer.%目的:探讨血清肿瘤标志物癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)、鳞状细胞癌相关抗原(SCCA)联合检测在肺癌诊断中的临床应用价值。方法68例肺癌患者为肺癌组,65例健康体检者为对照组,采用微粒子化学发光法检测血清中CEA、CYFRA21-1、NSE、SCCA含量,并比较肿瘤标志物单独或联合检测肺癌的灵敏度、特异度和准确度。结果肺癌组血清CEA、CYFRA21-1、NSE、SCCA含量均明显高于对照组(P<0.05),4种肿瘤标志物联合检测的灵敏度为81%,准确度为86%,明显高于单独检测,差异具有统计学意义(P<0.05)。结论血清CEA、CYFRA21-1、NSE、SCCA检测在肺癌诊断中具有临床应用价值,联合检测可提高诊断的灵敏度和准确度,且具有较好的特异度,有助于提高肺癌的检出率。

  10. Chronic exercise ameliorates the neuroinflammation in mice carrying NSE/htau23

    Energy Technology Data Exchange (ETDEWEB)

    Leem, Yea-Hyun, E-mail: leemyy@empas.com [Exercise Biochemistry Laboratory, Korea National Sport University, Seoul 138-763 (Korea, Republic of); Lee, Young-Ik, E-mail: lee0ik@hanmail.net [Department of Oriental Sports Medicine, Daegu Hanny University, Daegu 712-715 (Korea, Republic of); Son, Hee-Jeong, E-mail: son1106@paran.com [Exercise Physiology Laboratory, Korea National Sport University, Seoul 138-763 (Korea, Republic of); Lee, Sang-Ho, E-mail: run2025@hanmail.net [Department of Sports for All, Kangnam University, Yongin 446-702 (Korea, Republic of)

    2011-03-18

    Research highlights: {yields} The progress of neurodegeration are directly linked to the neuroinflammatory response. {yields} We investigate whether exercise improves the neuroinflammation using T{sub g}-NSE/htau23 mice. {yields} This provides insights that exercise may beneficial effects on the neuroinflammatory disorders. -- Abstract: The objective of the present study was to investigate whether chronic endurance exercise attenuates the neuroinflammation in the brain of mice with NSE/htau23. In this study, the tau-transgenic (Tg) mouse, Tg-NSE/htau23, which over expresses human Tau23 in its brain, was subjected to chronic exercise for 3 months, from 16 months of age. The brains of Tg mice exhibited increased immunoreactivity and active morphological changes in GFAP (astrocyte marker) and MAC-1 (microglia marker) expression in an age-dependent manner. To identify the effects of chronic exercise on gliosis, the exercised Tg mice groups were treadmill run at a speed of 12 m/min (intermediate exercise group) or 19 m/min (high exercise group) for 1 h/day and 5 days/week during the 3 month period. The neuroinflammatory response characterized by activated astroglia and microglia was significantly repressed in the exercised Tg mice in an exercise intensity-dependent manner. In parallel, chronic exercise in Tg mice reduced the increased expression of TNF-{alpha}, IL-6, IL-1{beta}, COX-2, and iNOS. Consistently with these changes, the levels of phospho-p38 and phospho-ERK were markedly downregulated in the brain of Tg mice after exercise. In addition, nuclear NF-{kappa}B activity was profoundly reduced after chronic exercise in an exercise intensity-dependent manner. These findings suggest that chronic endurance exercise may alleviate neuroinflammation in the Tau pathology of Alzheimer's disease.

  11. Analysis of the Clinical Feature of 383 Patients with Lung Cancer%383例原发性支气管肺癌临床特征的分析研究

    Institute of Scientific and Technical Information of China (English)

    黎银焕; 周燕斌; 黄琬玲; 蔡兴东; 曾庆理; 黄丽霞

    2011-01-01

    Objective To understand the clinical feature of lung cancer.Methods The clinical data of 383 lung cancer patients who registered in our hospital between 2007 and 2008 were analyzed retrospectively.Results The median age of lung cancer patients was 59 years old.50.1% of them had a history of smoking,among 93.8% were males.Coughing,expectoration,emaciation, hemoptysis, chest pain and dyspnea were the most common symptoms.The levels of serum neuron-specific enolase (NSE) and cytokeratin 19 fragment( CYFRA21-1 ) in males were higher than those in females, and the levels of serum CYFRA21-1 in senile were higher than those in non-senile.The levels of serum NSE and CYFRA21-1 in smokers were higher than those in non-smokers ,while they were higher in patients with central type than those in patients with peripheral type.The levels of serum NSE in small cell lung cancer patients were higher than those in non-small cell lung cancer patients.The levels of serum carcinoembryonic antigen (CEA) in patients with adenocarcinoma were higher than those in patients with squamous cell carcinoma,while the levels of serum CYFRA21-1 were higher in the latter.The more advanced the disease was,the higher the levels of serum CEA, NSE and CYFRA21-1 were.Carcinoma was most commonly seen in the upper lobes of both sides of the lungs.68.6%of lung cancer belonged to peripheral type.Adenocarcinoma and squamous cell carcinoma were the most common pathological type.66.0% of lung cancer patients were in advanced stage when diagnosed, and 55.5% had metastasis to mediastinal lymph nodes.Bones were the most common sites of metastasis.Conclusion The clinical manifestations were various in lung cancer patients,most of whom were in advanced stage when diagnosis was confirmed.Thus our knowledge to the disease should be enhanced,and early diagnosis for lung cancer should be made.The combined detection of serum CEA,NSE and CYFRA21-1 could be helpful to identify the histological types and stages of

  12. Diagnostic value of four serum tumor markers in lung cancer%血清四项肿瘤标志物检测对肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    吴凤丽; 马晓光

    2014-01-01

    目的:探讨血清中癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA21-1)、鳞状细胞癌抗原(SCCA)检测对肺癌的诊断价值。方法分别测定98例肺癌患者和30例健康者血清中CEA、NSE、CYFRA21-1、SCCA的水平,计算各自的阳性率、灵敏度、特异度及准确度,并进行统计学分析。结果与对照组比较,不同病理分型肺癌各组中四种血清肿瘤标志物水平显著升高(P<0.05);CEA、NSE、CYFRA21-1分别在腺癌、小细胞肺癌、鳞癌中的灵敏度最高;四种血清肿瘤标志物联合检测用于诊断的灵敏度和准确度增高。结论检测肺癌患者血清CEA、NSE、CYFRA21-1、SCCA 的水平,有助于肺癌病理分型、临床诊断及鉴别诊断,CEA、NSE分别为肺腺癌、小细胞肺癌诊断的肿瘤标志物;CYFRA21-1与SCCA可作为肺鳞癌诊断的肿瘤标志物;四项肿瘤标志物的联合检测可提高诊断的灵敏度和准确度。%Objective To investigate the diagnostic value of serum tumor markers carcinoembryonic antigen (CEA) ,neuron spe-cific enolase(NSE) ,cytokeratin-19 fragments(CYFRA21-1) ,squamous cell carcinoma antigen(SCCA)in the lung cancer .Methods By using electro chemiluminescence immunoassay ,the concentrations of CEA ,NSE ,CYFRA21-1 and SCCA were determined in 98 cases of lung cancer and 30 cases of healthy control group ,and the positive rate ,sensitivity ,specificity ,accuracy were calculated .Re-sults In different lung cancer pathological groups ,the concentrations of the 4 serum tumor markers were significantly higher than the control group(P<0 .05) .CEA had the highest diagnostic sensitivity for detecting adeno carcinoma ,NSE had the highest diag-nostic sensitivity for detecting small cell lung cancers ,CYFRA21-1 had the highest diagnostic sensitivity for detecting squamous carcinoma .The combined tests of four serum tumor markers had higher diagnostic sensitivity

  13. Body image and extreme attitudes toward weight in Brazilian schoolchildren (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Rafael Moreira Claro

    2014-01-01

    Full Text Available INTRODUCTION: Adolescence is a period characterized by changes such as accelerated physical growth and sexual development. Besides having to deal with these changes, adolescents are faced with beauty standards and extreme valorization of physical appearance. OBJECTIVE: This article aims to describe body image and the practice of extreme attitudes regarding weight in Brazilian students. METHODS: Data from the National Survey of School Health (PeNSE 2012 were used in this study. PeNSE 2012 has a representative sample of students in the 9th grade of elementary school in public and private schools across the country. A self-administered questionnaire on body image, practice of extreme measures in relation to weight and sociodemographic data was used. Body image and the practice of extreme attitudes were described for the total sample and according to gender. Poisson regression analyzes were used to identify differences in the practices of extreme attitudes between the different types of body image. RESULTS: More than 38% of the adolescents did not consider their body image as normal. Over 15% of the students referred to carry out extreme weight control practices, combining practices to loose and gain weight. Adolescents who considered themselves fat presented frequency of extreme practices for weight loss 92% higher than that shown by individuals who considered themselves normal. Similarly, adolescents who considered themselves thin presented frequency of extreme attitudes to gain weight (9.7% higher than that shown by students who considered themselves normal (5.6%. CONCLUSIONS: The high frequency of extreme weight control practices among Brazilian adolescents is alarming and should be subject of measures in health and education fields.

  14. Value of Serum ProGRP,NSE and CEA Detection in Diagnosis of Small Cell Lung Cancer%血清 ProGRP,NSE 和 CEA 检测对小细胞肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    孙树凯; 田清武; 刘入华; 申井利; 翟玉娥; 翟木绪

    2016-01-01

    目的:探讨血清胃泌素释放肽前体(ProGRP)、神经元特异性烯醇化酶(NSE)和癌胚抗原(CEA)联合检测对小细胞肺癌(SCLC)的诊断价值。方法471例肺部肿瘤患者均来自呼吸内科和胸外科住院患者,另从健康查体中心随机选取162例健康人作为对照组。所有研究对象均空腹采血,采用电化学发光法检测其血清 ProGRP,NSE 和 CEA 水平,绘制ROC 曲线并计算曲线下面积(the area under the curve,AUC)。结果SCLC 患者血清 ProGRP 和 NSE 水平显著高于非小细胞肺癌组、肺良性病变组和正常对照组(P <0.01)。SCLC 患者血清 CEA 水平显著高于肺良性病变组和正常对照组(P<0.05)。ProGRP,NSE 和 CEA 诊断 SCLC 的 AUC 分别为0.933,0.777和0.554。ProGRP,NSE 和 CEA 诊断 SCLC 的敏感度分别为82.6%,60.4%和41.6%,特异度分别为95.2%,83.3%和71.7%。联合检测 ProGRP,NSE 和 CEA 诊断SCLC 的敏感度为91.3%,特异度为65.3%。结论血清 ProGRP 检测对 SCLC 具有较高的诊断价值;ProGRP,NSE 和CEA 的联合检测有助于 SCLC 的早期诊断。%Objective To investigate the diagnostic value of combined detection of serum gastrin-releasing peptide precursor (ProGRP),neuron specific enolization enzyme(NSE)and carcinoembryonicantigen(CEA)in small cell lung cancer(SCLC). Methods 471 patients with lung tumor from department of respiratory medicine and thoracic surgery and 162 healthy people from medical examination center were studied.Serum levels of ProGRP,NSE and CEA were detected by using electrochemi-cal luminescence method.ROC curves were drawn and the area under the curve (AUC)was calculated.Results The levels of ProGRP and NSE were significantly higher in patients with SCLC than those in NSCLC,lung benign disease group and normal control group (P <0.01).The levels of CEA were significantly higher in SCLC than those in patients with lung be-nign disease

  15. Diagnostic value of multiple tumor markers for patients with esophageal carcinoma.

    Science.gov (United States)

    Zhang, Jun; Zhu, Zhenli; Liu, Yan; Jin, Xueyuan; Xu, Zhiwei; Yu, Qiuyan; Li, Ke

    2015-01-01

    Various studies assessing the diagnostic value of serum tumor markers in patients with esophageal cancer remain controversial. This study aims to comprehensively and quantitatively summarize the potential diagnostic value of 5 serum tumour markers in esophageal cancer. We systematically searched PubMed, Embase, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biomedical Database (CBM), through February 28, 2013, without language restriction. Studies were assessed for quality using QUADAS (quality assessment of studies of diagnostic accuracy). The positive likelihood ratio (PLR) and negative likelihood ratio (NLR) were pooled separately and compared with overall accuracy measures using diagnostic odds ratios (DORs) and symmetric summary receiver operating characteristic (SROC) curves. Of 4391 studies initially identified, 44 eligible studies including five tumor markers met the inclusion criteria for the meta-analysis, while meta-analysis could not be conducted for 12 other tumor markers. Approximately 79.55% (35/44) of the included studies were of relatively high quality (QUADAS score≥7). The summary estimates of the positive likelihood ratio (PLR), negative likelihood ratio (NLR) and diagnostic odds ratio (DOR) for diagnosing EC were as follows: CEA, 5.94/0.76/9.26; Cyfra21-1, 12.110.59/22.27; p53 antibody, 6.71/0.75/9.60; SCC-Ag, 7.66/0.68/12.41; and VEGF-C, 0.74/0.37/8.12. The estimated summary receiver operating characteristic curves showed that the performance of all five tumor markers was reasonable. The current evidence suggests that CEA, Cyfra21-1, p53, SCC-Ag and VEGF-C have a potential diagnostic value for esophageal carcinoma.

  16. To evaluate the combined detection of multiple tumor markers in early diagnosis of esophageal cancer%血清肿瘤标志物联合检测在食管癌早期诊断中的价值分析

    Institute of Scientific and Technical Information of China (English)

    侯激流

    2012-01-01

    目的 探讨联合检测肿瘤标志物癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)和细胞角蛋白19片段抗原(CYFRA21-1)在食管癌早期诊断中的价值.方法 采用罗氏电化学发光免疫系统检测72例食管癌患者、71例正常健康者的血清CEA、CYFRA21-1、NSE水平.结果 CEA、CYFRA21-1、NSE在食管癌患者中的含量均明显高于健康人(P<0.05),并随着临床分期的增加而显著增加(P<0.05).三者在食管癌诊断中均具有较高的特异性,但敏感性均较低.而三者联检敏感性和特异性分别为81.9%和83.3%,敏感性明显提高.结论 CEA、NSE、CYFRA21-1可作为食管癌早期诊断的重要辅助指标,联合检测可提高诊断的敏感性,具有一定临床应用价值

  17. The K+-dependent asparaginase, NSE1, is crucial for plant growth and seed production in Lotus japonicus.

    Science.gov (United States)

    Credali, Alfredo; García-Calderón, Margarita; Dam, Svend; Perry, Jillian; Díaz-Quintana, Antonio; Parniske, Martin; Wang, Trevor L; Stougaard, Jens; Vega, José M; Márquez, Antonio J

    2013-01-01

    The physiological role of K(+)-dependent and K(+)-independent asparaginases in plants remains unclear, and the contribution from individual isoforms during development is poorly understood. We have used reverse genetics to assess the phenotypes produced by the deficiency of K(+)-dependent NSE1 asparaginase in the model legume Lotus japonicus. For this purpose, four different mutants were identified by TILLING and characterized, two of which affected the structure and function of the asparaginase molecule and caused asparagine accumulation. Plant growth and total seed weight of mature mutant seeds as well as the level of both legumin and convicilin seed storage proteins were affected in the mutants. The mutants isolated in the present work are the first of their type in legumes and have enabled us to demonstrate the importance of asparagine and K(+)-dependent NSE1 asparaginase for nitrogen remobilization and seed production in L. japonicus plants.

  18. The neonatal levels of TSB, NSE and CK-BB in autism spectrum disorder from Southern China

    Directory of Open Access Journals (Sweden)

    Lv Meng-na

    2016-01-01

    Full Text Available Background" Autism spectrum disorder (ASD is a serious neurodevelopmental disorder that impairs a child’s ability to communicate with others. It also includes restricted repetitive behaviors, interests and activities. Symptoms manifest before the age of 3. In the previous studies, we found structural abnormalities of the temporal lobe cortex. High spine densities were most commonly found in ASD subjects with lower levels of cognitive functioning. In the present study, we retrospectively analyzed medical records in relation to the neonatal levels of total serum bilirubin (TSB, neuron-specific enolase (NSE, creatine kinase brain band isoenzyme (CK-BB, and neonatal behavior in ASD patients from Southern China. Methods: A total of 80 patients with ASD (ASD group were screened for this retrospective study. Among them, 34 were low-functioning ASD (L-ASD group and 46 were high-functioning ASD (H-ASD group. Identification of the ASD cases was confirmed with a Revised Autism Diagnostic Inventory. For comparison with ASD cases, 80 normal neonates (control group were selected from the same period. Biochemical parameters, including TSB, NSE and CK-BB in the neonatal period and medical records on neonatal behavior were collected. Results: The levels of serum TSB, NSE and CK-BB in the ASD group were significantly higher when compared with those from the control group (P 0.05 in the ASD group. Conclusions: The neonatal levels of TSB, NSE and CK-BB in ASD from Southern China were significantly higher than those of healthy controls. These findings need to be investigated thoroughly by future studies with large sample.

  19. Experimentation and use of cigarette and other tobacco products among adolescents in the Brazilian state capitals (PeNSE 2012)

    OpenAIRE

    2014-01-01

    INTRODUCTION: Nicotine dependence establishes itself more rapidly among adolescents than among adults. Tobacco occupies the fourth place in the rank of main risk factors for non-communicable diseases in the continent. Studies reveal that other forms of tobacco use have increased among adolescents. METHODS: Were included the 9th grade students from the 26 State Capitals and the Federal District. who were participants of the National Adolescent School-based Health Survey (PeNSE), in 2012. ...

  20. Consumption of sugar-rich food products among Brazilian students:National School Health Survey (PeNSE 2012)

    OpenAIRE

    2015-01-01

    Abstract This study aimed to analyze the consumption of high-sugar foods by Brazilian schoolchildren and to identify associated factors, based on data from the National School Health Survey (PeNSE 2012). Consumption of these foods was classified as: do not consume sweets and soft drinks regularly; consume sweets or soft drinks regularly; and consume sweets and soft drinks regularly. Its association with sociodemographic information, eating habits, and family contexts were investigated via mul...

  1. Utility of NSE, ProGRP and LDH in Diagnosis and Treatment
in Patients with Small Cell Lung Cancer

    Directory of Open Access Journals (Sweden)

    Yan PENG

    2016-09-01

    Full Text Available Background and objective Small cell lung cancer (SCLC is a rapidly growing tumor with characteristic of neuroendocrine cellular function. Neuron specific enolase (NSE, pro-gastrin-releasing peptide (ProGRP and lactic dehydrogenase (LDH are valuable in diagnosis and treatment of SCLC. By analyzing the variation of NSE, ProGRP and LDH before and after treatment, the aim of this study is to investigate the efficacy of tumor markers in diagnostic staging, therapeutic evaluation and prediction of disease relapsing. Methods Patients with SCLC who receiving the first line chemotherapy in Cancer Hospital, Chinese Academy of Medical Sciences were enrolled and retrospectively analyzed. Clinical characteristic (includes NSE, ProGRP and LDH level before and after 2 cycles chemotherapy, efficacy evaluation, progression-free survival (PFS were analyzed. Results Before treatment, Serum NSE, ProGRP and LDH in patients with extensive disease (ED were significantly higher than those with limited disease (LD(all P4 cycles chemotherapy and with obvious decrease of ProGRP than those who accepted ≤4 cycles chemotherapy and with less obvious decrease of ProGRP in LD group; ED patients with no more than 2 distant metastasis, normal LDH level before treatment and obvious decrease of ProGRP after chemotherapy had lower short term relapse risk. In addition, the types of relapse (sensitive relapse, drug resistance relapse and refractory relapse were negatively correlated with decrease of ProGRP (P=0.044. By multivariate analysis, numbers of chemotherapy cycle was independent prognostic factor for PFS in LD SCLC; numbers of distant metastasis and decrease of ProGRP were independent prognostic factors for PFS in ED SCLC. Conclusion Increase level of serum tumor markers is related to tumor burden. Decrease level of ProGRP after treatment may prognose efficacy and relapse risk.

  2. Sexual behavior among Brazilian adolescents, National Adolescent School-based Health Survey (PeNSE 2012).

    Science.gov (United States)

    Oliveira-Campos, Maryane; Nunes, Marília Lavocart; Madeira, Fátima de Carvalho; Santos, Maria Goreth; Bregmann, Silvia Reise; Malta, Deborah Carvalho; Giatti, Luana; Barreto, Sandhi Maria

    2014-01-01

    This study describes the sexual behavior among students who participated in the National Adolescent School-based Health Survey (PeNSE) 2012 and investigates whether social inequalities, the use of psychoactive substances and the dissemination of information on sexual and reproductive health in school are associated with differences in behavior. The response variable was the sexual behavior described in three categories (never had sexual intercourse, had protected sexual intercourse, had unprotected sexual intercourse). The explanatory variables were grouped into socio- demographic characteristics, substance use and information on sexual and reproductive health in school. Variables associated with the conduct and unprotected sex were identified through multinomial logistic regression, using "never had sexual intercourse" as a reference. Over nearly a quarter of the adolescents have had sexual intercourse in life, being more frequent among boys. About 25% did not use a condom in the last intercourse. Low maternal education and work increased the chance of risky sexual behavior. Any chance of protected and unprotected sex increased with the number of psychoactive substances used. Among those who don't receive guidance on the prevention of pregnancy in school, the chance to have sexual intercourse increased, with the largest magnitude for unprotected sex (OR = 1.41 and OR = 1.87 ). The information on preventing pregnancy and STD/AIDS need to be disseminated before the 9th grade. Social inequalities negatively affect risky sexual behavior. Substance use is strongly associated with unprotected sex. Information on the prevention of pregnancy and STD/AIDS need to be disseminated early.

  3. Significance of combined detection of serum tumor markers in the diagnosis of NSCLC%肿瘤标志物联合检测在非小细胞肺癌诊断中的应用价值

    Institute of Scientific and Technical Information of China (English)

    蒙飞; 王恒; 梁鑫

    2014-01-01

    目的:探讨神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA21-1)、癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原19-9(CA19-9)和血清铁蛋白(SF)联合检测在非小细胞肺癌(NSCLC)诊断和鉴别诊断中的应用价值。方法采用化学发光免疫分析法检测52例NSCLC患者(NSCLC组),35例肺部良性疾病患者(肺部良性疾病组)及44例健康体检者(对照组)血清中NSE、CYFRA21-1、CEA、CA125、CA19-9和SF的水平,并进行统计学分析。结果 NSCLC组血清中NSE、CY-FRA21-1、CEA、CA125、CA19-9和SF的检测水平均明显高于肺部良性疾病组和对照组,差异有统计学意义(P<0.01);腺癌患者CEA水平明显高于鳞癌患者,鳞癌患者CYFRA21-1的水平明显高于腺癌患者,差异均有统计学意义(P<0.05);6项肿瘤标志物联合检测NSCLC的灵敏度为96.2%,特异性为87.3%,准确性为90.8%,均优于任何单项肿瘤标志物检测。结论肿瘤标志物联合检测对NSCLC的辅助诊断有一定价值,可明显提高诊断灵敏度和准确性。%Objective To investigate the diagnostic significance of combined detection of neuron specific enolase (NSE ) , cytokeratin 19 fragment(CYFRA21-1) ,carcinoembryonic antigen(CEA) ,carbohydrate antigen 125(CA125) ,carbohydrate antigen 19-9(CA19-9) and serum ferritin(SF) in non-small-cell lung cancer(NSCLC) .Methods NSE ,CYFRA21-1 ,CEA ,CA125 ,CA19-9 and SF were detected by chemiluminescent immunoassay in 52 patients with NSCLC(NSCLC group) ,35 patients with benign lung diseases(benign lung diseases group) and 44 healthy controls(control group) .Results These serum tumor markers in NSCLC group were significantly higher than those in benign lung diseases group and control group(P<0 .01) .Serum level of CEA was ob-viously higher ,while CYFRA21-1 was lower in patients with adenocarcinoma than those in patients with squamous carcinoma

  4. Characteristics of metal-tolerant plant growth-promoting yeast (Cryptococcus sp. NSE1) and its influence on Cd hyperaccumulator Sedum plumbizincicola.

    Science.gov (United States)

    Liu, Wuxing; Wang, Beibei; Wang, Qingling; Hou, Jinyu; Wu, Longhua; Wood, Jennifer L; Luo, Yongming; Franks, Ashley E

    2016-09-01

    Plant growth-promoting yeasts are often over looked as a mechanism to improve phytoremediation of heavy metals. In this study, Cryptococcus sp. NSE1, a Cd-tolerant yeast with plant growth capabilities, was isolated from the rhizosphere of the heavy metal hyperaccumulator Sedum plumbizincicola. The yeast exhibited strong tolerance to a range of heavy metals including Cd, Cu, and Zn on plate assays. The adsorption rate Cd, Cu, Zn by NSE1 was 26.1, 13.2, and 25.2 %, respectively. Irregular spines were formed on the surface of NSE1 when grown in MSM medium supplemented with 200 mg L(-1) Cd. NSE1 was capable of utilizing 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source and was capable of solubilization of inorganic phosphate at rates of 195.2 mg L(-1). Field experiments demonstrated that NSE1 increased phytoremediation by increasing the biomass of Cd hyperaccumulator S. plumbizincicola (46 %, p Cd accumulation by S. plumbizincicola was increased from 19.6 to 31.1 mg m(-2) though no difference in the concentration of Cd in the shoot biomass was observed between NSE1 and control. A Cd accumulation ratio of 38.0 % for NSE1 and 17.2 % for control was observed. The HCl-extractable Cd and CaCl2-extractable Cd concentration in the soil of the NSE1 treatment were reduced by 39.2 and 29.5 %, respectively. Community-level physiology profiling, assessed using Biolog Eco plates, indicated functional changes to the rhizosphere community inoculated with NSE1 by average well color development (AWCD) and measurement of richness (diversity). Values of Shannon-Weiner index, Simpson index, and McIntosh index showed a slight but no significant increases. These results indicate that inoculation of NSE1 could increase the shoot biomass of S. plumbizincicola, enhance the Cd accumulation in S. plumbizincicola, and decrease the available heavy metal content in soils significantly without overall significant changes to the microbial community.

  5. Validation of an algorithm able to differentiate small-cell lung cancer (SCLC) from non-small-cell lung cancer (NSCLC) patients by means of a tumour marker panel: analysis of the errors.

    OpenAIRE

    Paone, G; De Angelis, G.; Portalone, L.; Greco, S.; Giosué, S.; Taglienti, A.; Bisetti, A; Ameglio, F

    1997-01-01

    By means of a mathematical score previously generated by discriminant analysis on 90 lung cancer patients, a new and larger group of 261 subjects [209 with non-small-cell lung cancer (NSCLC) and 52 with small-cell lung cancer (SCLC)] was analysed to confirm the ability of the method to distinguish between these two types of cancers. The score, which included the serum neuron-specific enolase (NSE) and CYFRA-21.1 levels, permitted correct classification of 93% of the patients. When the misclas...

  6. Clinical significance of multiple serum tumor markers combined detection in the diagnosis of lung cancer%血清多项肿瘤标志物联合检测在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    敖素华; 杨峰

    2013-01-01

    目的:探讨癌胚抗原(CEA)、细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)联合检测在肺癌诊断中的临床价值。方法采用化学发光法对61例肺癌患者和38例肺部良性病变患者血清CEA、CYFRA21-1、NSE水平进行检测。根据接受者工作特性曲线(ROC),确定最佳截断点及诊断灵敏度、特异度。结果肺癌组患者血清CEA、CYFRA21-1、NSE水平均明显高于肺部良性病变组,差异有统计学意义(P<0.05);3项肿瘤标志物在不同病理类型肺癌中的水平差异无统计学意义(P>0.05);CEA诊断肺癌最佳截断点为3.50 ng/mL ,灵敏度和特异度分别为52.5%、94.7%;CYFRA21-1最佳截断点为5.21 ng/mL ,灵敏度和特异度分别为67.2%、93.1%;NSE最佳截断点为18.09 ng/mL ,灵敏度和特异度分别为77.0%、52.6%。3项指标联合检测的灵敏度为96.7%,高于3项单独检测时的灵敏度(P<0.05)。结论检测CEA、CYFRA21-1和NSE对肺癌的诊断有一定价值,3者联合检测有明显的互补性,可提高肺癌诊断的灵敏度。%Objective To study the clinical significance of Carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CY-FRA21-1) ,neuron specific enolization enzyme (NSE) combined detection in the diagnosis of lung cancer .Methods Using the Chemiluminescence to detect the level of CEA ,NSE and CYFRA21-1 in 61 patient with lung cancer (lung cancer group) and 38 pa-tients with benign diseases(control group) .All datas were analyzed with t test .Optimal cut-off point and diagnostic sensitivity ,spe-cificity were obtained by receiver operating characteristic curve (ROC) .Results The level of serum CEA ,NSE and CYFRA21-1 in Lung cancer group was significantly higher than that in control group (P0 .05) .The cut-off point of CEA was 3 .50 ng/mL ,sensitivity and speciality were 52 .5% ,94 .7% respectively .The cut-off point of

  7. 肿瘤标志物动态变化在肺癌疗效判断与随访中的意义%Significance of the dynamic changes of tumor markers in clinical judgment and follow-up of lung cancer

    Institute of Scientific and Technical Information of China (English)

    张红军; 顾兴; 刘伟; 谢永宏; 金发光; 任志超; 曹春锋

    2016-01-01

    目的:探讨癌胚抗原( carcino-embryonic antigen, CEA)、铁蛋白( ferritin, FRT)、糖类抗原125(carbohydrate antigen 125, CA125)、神经元特异性烯醇化酶(neuron specific enolase, NSE)和细胞角蛋19片段( cytokeratin19 fragment, CYFRA21-1)等5种血清学肿瘤标志物( tumor markers,TM)的动态变化在肺癌疗效判断与随访中的意义。方法选择唐都医院呼吸内科经治的170例中晚期肺癌患者,采用电化学发光免疫分析法检测CEA、FRT、CA125、NSE和CYFRA21-1等5种血清学TM,观察不同TM在肺癌治疗过程中的动态变化水平。结果治疗2周期后,CA125在各肺癌组、CYFRA21-1在鳞癌和腺癌及未分类癌、NSE在小细胞癌及神经内分泌癌、CEA在腺癌和未分类癌及神经内分泌癌、FRT在鳞癌治疗中呈下降趋势。结论血清TM水平的动态变化,在一定程度上反映肿瘤的活动情况及疗效,可用于肺癌疗效判断与随访。%Objective Levels of the carcino-embryonic antigen( CEA) , ferritin ( FRT) , carbohydrate antigen 125 ( CA125 ) , neuron-specific enolase ( NSE ) and cytokeratin 19 fragments ( CYFRA21-1 ) were studied in lung cancer diagnosis and treatment, to evaluate the value of different tumor markers ( TM ) in therapeutic efficacy and follow-up of lung cancer. Methods All 170 cases of patients with advanced lung cancer were recruited in Department of Respiratory Medicine of Tangdu Hospital. CEA, FRT, CA125, NSE and CYFRA21-1 were measured by electro-chemiluminescence Immunoassay ( ECLI ) . The levels of them were observed in the retrospective study. Results There was a certain downward trend change of CA125 in the lung cancer groups, CYFRA21-1 in squamous cell carcinoma and adenocarcinoma and non-classified carcinoma, NSE in small cell carcinoma and neuroendocrine carcinoma, CEA in adenocarcinoma and non-classified carcinoma and neuroendocrine carcinoma, FRT in treatment of squamous cell carcinoma after 2 cycles of treatment. Conclusion

  8. NSE-1飞行员颈肌训练器的研制及其应用%Research, Design and Application of Model NSE-1 Neck Muscle Training Machine for Pilots

    Institute of Scientific and Technical Information of China (English)

    成海平; 王致洁; 柳松杨; 杨毅; 赵广; 丛红; 韩学平; 刘敏; 俞梦孙

    2011-01-01

    空军飞行员暴露于高G载荷下导致颈部疼痛是一个特殊的职业问题,为了减少这方面的问题有必要进行特殊的颈肌强度训练.为了安全有效地进行颈肌强度训练,提供颈肌强度训练的最佳阻抗是非常重要的.本研究设计了NSE-1型飞行员颈肌训练器.为实现可变阻力和可变速度(CVR)肌力训练以及等长训练,本研究采用了自适应液压技术、传感技术、视觉和听觉生物反馈技术和信号处理技术,系统由机械部分(前屈后伸和左右侧屈训练器座椅、液压组件、力矩传递组件)、信号处理和生物反馈软件组成.为验证该训练器的有效性和安全性,对11名志愿者进行前屈后伸和左右侧屈的CVR训练和等长训练,每周三次,共6周.前屈、后伸、左右侧屈肌群的相对强度分别增长70.8%、83.7%、78.6%和75.2%.结果表明:应用NSE-1飞行员颈肌训练器进行颈肌训练,能安全高效地提高颈肌各肌群的强度.%Pain in the cervical region of air force pilots, who are exposed to high G-forces, is a specifically occupational health problem. To minimize neck problems, the cervical muscles need specific strength exercise. It is important that the training for the neck must be carried out with optimal resistance in exercises. The model NSE-1 neck training machine for pilots was designed for neck strengthening exercises under safe and effective conditions. In order to realize the functions of changeable velocity and resistant (CVR) training and neck isometric contractive exercises, the techniques of adaptive hydraulics, sensor, optic and auditory biological feedback, and signal processing were applied to this machine. The training system mainly consists of mechanical parts (including the chair of flexion and extension, the chair of right and left lateral flexion, the components of hydraulics and torque transformer, etc. ) , and the software of signal processing and biological feedback. Eleven

  9. Softening of phospholipid membranes by the adhesion of silica nanoparticles - as seen by neutron spin-echo (NSE)

    Science.gov (United States)

    Hoffmann, Ingo; Michel, Raphael; Sharp, Melissa; Holderer, Olaf; Appavou, Marie-Sousai; Polzer, Frank; Farago, Bela; Gradzielski, Michael

    2014-05-01

    The interactions between nanoparticles and vesicles are of significant interest both from a fundamental as well as from a practical point of view, as vesicles can serve as a model system for cell membranes. Accordingly the effect of nanoparticles that bind to the vesicle bilayer is very important with respect to understanding their biological impact and also may shed some light on the mechanisms behind the effect of nanotoxicity. In this study we have investigated the influence of small adsorbed silica nanoparticles (SiNPs) on the structure of zwitterionic DOPC vesicles. By a combination of SANS, cryo-TEM, and DLS, we observed that the SiNPs are bound to the outer vesicle surface without significantly affecting the vesicle structure. Most interestingly, by means of neutron spin-echo (NSE) local bilayer fluctuations were studied and one finds a small but marked decrease of the membrane rigidity upon binding of the nanoparticles. This surprising finding may be a relevant aspect for the further understanding of the effects that nanoparticles have on phospholipid bilayers.The interactions between nanoparticles and vesicles are of significant interest both from a fundamental as well as from a practical point of view, as vesicles can serve as a model system for cell membranes. Accordingly the effect of nanoparticles that bind to the vesicle bilayer is very important with respect to understanding their biological impact and also may shed some light on the mechanisms behind the effect of nanotoxicity. In this study we have investigated the influence of small adsorbed silica nanoparticles (SiNPs) on the structure of zwitterionic DOPC vesicles. By a combination of SANS, cryo-TEM, and DLS, we observed that the SiNPs are bound to the outer vesicle surface without significantly affecting the vesicle structure. Most interestingly, by means of neutron spin-echo (NSE) local bilayer fluctuations were studied and one finds a small but marked decrease of the membrane rigidity upon

  10. Clinical value evaluation of serum tumor markers detection in patients with lung cancer%肺癌患者血清肿瘤标志物检测的临床价值评价

    Institute of Scientific and Technical Information of China (English)

    吴旭波

    2016-01-01

    Objective:To investigate the clinical value of serum tumor markers detection in the diagnosis of patients with lung cancer.Methods:64 cases of patients with suspected lung cancer were as the clinical study object and whose serum levels of cytokeratin 19 fragment (CYFRA21-1),carcinoembryonic antigen (CEA),neuron specific enolase (NSE),pro-gastrin-releasing peptide were detected,and the sensitivity and specificity of individual four tumor markers detection and joint detection were evaluated.Results:The every indexes of patients with lung cancer were significantly higher than that of patients without lung cancer,and the differences were statistically significant(P<0.05).The positive rate,sensitivity and specificity of each marker were significantly lower than that of the joint detection,and the differences were statistically significant(P<0.05).Conclusion:Joint detection of CYFRA21-1,CEA,NSE,ProGRP could assist the diagnosis of lung cancer effectively and provide objective reference for the prognosis of patients with lung cancer.%目的:探讨血清肿瘤标志物检测对诊断肺癌患者的临床价值。方法:收治疑似肺癌患者64例作为临床研究对象,分别检测血清细胞角蛋白19片段(CYFRA21-1)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、胃泌素释放肽前体(ProGRP)的水平,评价四项肿瘤标志物单独检测及联合检查的敏感度与特异性。结果:肺癌患者的各项指标水平均显著高于非肺癌患者,差异具有统计学意义(P<0.05)。各标志物单独检测的阳性率、敏感度、特异度明显低于联合检测,差异具有统计学意义(P<0.05)。结论:联合检测 CYFRA21-1、CEA、NSE、ProGRP 可有效辅助诊断肺癌,为肺癌患者的预后提供客观的参考依据。

  11. Antiplasmodial and Cytotoxic Triterpenoids from the Bark of the Cameroonian Medicinal Plant Entandrophragma congoënse.

    Science.gov (United States)

    Happi, Gervais Mouthé; Kouam, Simeon Fogue; Talontsi, Ferdinand Mouafo; Lamshöft, Marc; Zühlke, Sebastian; Bauer, Jonathan O; Strohmann, Carsten; Spiteller, Michael

    2015-04-24

    Eight new triterpenoids, prototiamins A-G (1-6, 9) and seco-tiaminic acid A (10), were isolated along with four known compounds from the bark of Entandrophragma congoënse. Their structures were elucidated by means of HRMS and different NMR techniques and chemical transformations. Assignments of relative and absolute configurations for the new compounds were achieved using NOESY experiments and by chemical modification including the advanced Mosher's method. Additionally, the structure and relative configuration of compound 3 were confirmed by single-crystal X-ray diffraction analysis. Compounds 1, 3, and 5 displayed significant in vitro antiplasmodial activity against the erythrocytic stages of chloroquine-sensitive Plasmodium falciparum strain NF54. Prototiamin C (3) was the most potent of the compounds isolated, with an IC50 value of 0.44 μM. All compounds tested showed low cytotoxicity for the L6 rat skeletal myoblast cell line.

  12. Uncovering the Internal Structure of the Indian Financial Market: Large Cross-correlation Behavior in the NSE

    Science.gov (United States)

    Sinha, Sitabhra; Pan, Raj Kumar

    The cross-correlations between price fluctuations of 201 frequently traded stocks in the National Stock Exchange (NSE) of India are analyzed in this paper. We use daily closing prices for the period 1996-2006, which coincides with the period of rapid transformation of the market following liberalization. The eigenvalue distribution of the cross-correlation matrix, C, of NSE is found to be similar to that of developed markets, such as the New York Stock Exchange (NYSE): the majority of eigenvalues fall within the bounds expected for a random matrix constructed from mutually uncorrelated time series. Of the few largest eigenvalues that deviate from the bulk, the largest is identified with market-wide movements. The intermediate eigenvalues that occur between the largest and the bulk have been associated in NYSE with specific business sectors with strong intra-group interactions. However, in the Indian market, these deviating eigenvalues are comparatively very few and lie much closer to the bulk. We propose that this is because of the relative lack of distinct sector identity in the market, with the movement of stocks dominantly influenced by the overall market trend. This is shown by explicit construction of the interaction network in the market, first by generating the minimum spanning tree from the unfiltered correlation matrix, and later, using an improved method of generating the graph after filtering out the market mode and random effects from the data. Both methods show, compared to developed markets, the relative absence of clusters of co-moving stocks that belong to the same business sector. This is consistent with the general belief that emerging markets tend to be more correlated than developed markets.

  13. The K+ dependent asparaginase, NSE1, is crucial for plant growth and seed production in Lotus japonicus

    DEFF Research Database (Denmark)

    Credali, Alfredo; Garcia-Calderón, Margarita; Dam, Svend Secher

    2013-01-01

    The physiological role of K+-dependent and K+-independent asparaginases in plants remains unclear, and the contribution from individual isoforms during development is poorly understood. We have used reverse genetics to assess the phenotypes produced by the deficiency of K+-dependent NSE1 asparagi...

  14. 血清肿瘤标志物联合检测对肺癌诊断中的临床价值%The combined detection of serum tumor markers in diagnosis of lung cancer in the clinical value

    Institute of Scientific and Technical Information of China (English)

    孙娟; 谷秀娟; 阮彩莲

    2012-01-01

    目的:探讨四种肿瘤标志物癌胚抗原(CEA)、糖类抗原(CA12-5)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA21-1)联合应用在肺癌诊断中的临床价值.方法:采用电化学发光法检测189例肺部良性病患者.100例经病理确诊肺癌患者、60例健康人的血清CEA、CA125、NSE、CYFRA21-1的水平及阳性率,对比分析四种肿瘤标志物联合及单独应用的敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性似然比、阴性似然比等.结果:肺癌组CEA、CAt25、NSE、CYFRA21-1水平均明显高于良性病变组(P<0.05)及健康对照组(P<0.01).结论:四项联合检测可提高肺癌诊断的敏感度和准确度,优于其它单项检测和其它联合检测,具有较高的临床应用价值.%Objective Discuss so study the clinical value of four kinds of tumor markers including carcinoembryonic antigen(CEA),car-bohydrate antigen(CA12-5),neuron specific enlace(NSE),cytokeratin 19 fragment(CYFRA21-1)combined detection in diagnosis of lung cancer. Methods Using electrochemical luminescence method detection in 189 patients with lung benign disease patients, 100 cases of pathologically confirmed lung cancer patients,60 healthy persons'serum CEA,CA125,NSE,CYFRA21-1 levels and positive rates.com-parative analysis of four kinds of tumor markers'joint and separate application of sensitivity.specificity,accuracy,positive predictive value, negative predictive value,positive likelihood ratio.negative likelihood ratio and soon on. Results The CEA、CA125、NSE、CYFRA21-1 levels in lung cancer group were significantly higher than that in benign disease group (P<0.05)and healthy control groups (P<0.01). Conclusion Joint detection can enhance the sensitivity and accuracy lung cancer diagnostic, better than other individual testing and other joint detection,it has high value in clinical application.

  15. Clinical Evaluation and Cost-Effectiveness Analysis of Serum Tumor Markers in Lung Cancer

    Directory of Open Access Journals (Sweden)

    Rong Wang

    2013-01-01

    Full Text Available The detection of serum tumor markers is valuable for the early diagnosis of lung cancer. Tumor markers are frequently used for the management of cancer patients. However, single markers are less efficient but marker combinations increase the cost, which is troublesome for clinics. To find an optimal serum marker combination panel that benefits the patients and the medical management system as well, four routine lung cancer serum markers (SCCA, NSE, CEA, and CYFRA21-1 were evaluated individually and in combination. Meanwhile, the costs and effects of these markers in clinical practice in China were assessed by cost-effectiveness analysis. As expected, combinations of these tumor markers improved their sensitivity for lung cancer and different combination panels had their own usefulness. NSE + CEA + CYFRA21-1 was the optimal combination panel with highest Youden’s index (0.64, higher sensitivity (75.76%, and specificity (88.57%, which can aid the clinical diagnosis of lung cancer. Nevertheless, the most cost-effective combination was SCCA + CEA, which can be used to screen the high-risk group.

  16. Clinical Significance of Folate Receptor-positive Circulating Tumor Cells Detected by Ligand-targeted Polymerase Chain Reaction in Lung Cancer

    Science.gov (United States)

    Wang, Lin; Wu, Chuanyong; Qiao, Lihua; Yu, Wenjun; Guo, Qiaomei; Zhao, Mingna; Yang, Guohua; Zhao, Hang; Lou, Jiatao

    2017-01-01

    Background: As the heterogeneity of CTCs is becoming increasingly better understood, it is clear that identifying particular subtypes of CTCs would be more relevant. Methods: We detected folate receptor (FR)-positive circulating tumor cells (FR+-CTCs) by a novel ligand-targeted polymerase chain reaction (LT-PCR) detection technique. Results: In the none-dynamic study, FR+-CTC levels of patients with lung cancer were significantly higher than controls (patients with benign lung diseases and healthy controls). With a threshold of 8.7 CTC units, FR+-CTC showed a sensitivity of 77.7% and specificity of 89.5% in the diagnosis of lung cancer. When compared with established clinical biomarkers including carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1), and neuron-specific enolase (NSE), FR+-CTC showed the highest diagnostic efficiency. Notably, the combination of FR+-CTC, CEA, NSE, and CYFRA21-1 could significantly improve the diagnostic efficacy in differentiating patients with lung cancer from benign lung disease. In our dynamic surveillance study, the CTC levels of 62 non-small cell lung cancer (NSCLC) patients decreased significantly after tumor resection. Conclusion: We established a LT-PCR-based FR+-CTC detection platform for patients with lung cancer that exhibits high sensitivity and specificity. This platform would be clinical useful in lung cancer diagnosis and treatment response assessment.

  17. Diagnosis Value of Combined Detection of Tumor Markers for Lung Caner%肿瘤标志物联合检测对肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    王智存; 胡强

    2012-01-01

    目的 探讨血清肿瘤标志物细胞角蛋白19片段(CYFRA21-1)、神经元特异性烯醇化酶(NSE)、癌胚抗原(CEA)、糖蛋白抗原125(CA125)、恶性肿瘤特异性生长因子(TSGF)联合检测对肺癌的诊断价值.方法 采用电化学发光法检测76例肺癌患者、90例肺部良性病变患者和86例健康体检者血清中的5种肿瘤标志物,并用统计学方法对比分析.结果 肺癌患者组5种肿瘤标志物水平明显高于肺良性病变组和健康体检组,差异有高度统计学意义(P<0.01).结论 CYFRA21-1、NSE、CEA、CA125和TSGF5种肿瘤标志物联合检测可有效提高肺癌诊断的敏感性,对肺癌早期诊断有重要的临床意义.%Objective To study the diagnosis value of combined detection of tumor markers CY-FRA21-1 ,NSE,CEA,CA125 and TSGF for lung cancer. Methods The electro-chemiluminescence immu-noassay was used to detect five tumor markers in serum of 76 patients with lung caner, 90 patients with benign diseases and 86 normal healthy people and statistics was used to conduct comparative analysis. Results The serum level of five tumor markers in lung caner group were higher than those in the benign disease group and the control group. There was statistics difference (P < 0. 05). Conclusions The combined detection of tumor markers CYFRA21-1、NSE、CEA、CA125 and TSGF may improve the sensitivity for diagnosis of lung cancer and has great clinical significance for early diagnosis of lung cancer.

  18. Dietary patterns of Brazilian adolescents: results of the Brazilian National School-Based Health Survey (PeNSE

    Directory of Open Access Journals (Sweden)

    Letícia Ferreira Tavares

    2014-12-01

    Full Text Available The objectives of this study were to identify dietary patterns in Brazilian adolescents, describe their distributions in Brazil's State capitals and Federal District, and analyze the correlations with the Municipal Human Development Index (HDI-M. The study analyzed a sample of 60,954 ninth-graders from public and private schools who participated in the National School-Based Health Survey (PeNSE in 2009. Cluster analysis was used to characterize dietary patterns. Three patterns were identified: healthy (27.7%, unhealthy (34.6%, and mixed (37.7%. Adolescents in the country's Southeast, South, and Central West regions showed a higher proportion of the healthy eating pattern. HDI-M showed a positive correlation with the healthy pattern and a negative correlation with the mixed pattern. The identification of different dietary patterns within and between regions and according to HDI-M highlights the need for better knowledge of each local context in terms of both the magnitude of events and the examination of determinants within these different realities.

  19. Dietary patterns of Brazilian adolescents: results of the Brazilian National School-Based Health Survey (PeNSE).

    Science.gov (United States)

    Tavares, Letícia Ferreira; Castro, Inês Rugani Ribeiro de; Levy, Renata Bertazzi; Cardoso, Letícia de Oliveira; Claro, Rafael Moreira

    2014-12-01

    The objectives of this study were to identify dietary patterns in Brazilian adolescents, describe their distributions in Brazil's State capitals and Federal District, and analyze the correlations with the Municipal Human Development Index (HDI-M). The study analyzed a sample of 60,954 ninth-graders from public and private schools who participated in the National School-Based Health Survey (PeNSE) in 2009. Cluster analysis was used to characterize dietary patterns. Three patterns were identified: healthy (27.7%), unhealthy (34.6%), and mixed (37.7%). Adolescents in the country's Southeast, South, and Central West regions showed a higher proportion of the healthy eating pattern. HDI-M showed a positive correlation with the healthy pattern and a negative correlation with the mixed pattern. The identification of different dietary patterns within and between regions and according to HDI-M highlights the need for better knowledge of each local context in terms of both the magnitude of events and the examination of determinants within these different realities.

  20. Consumption of sugar-rich food products among Brazilian students:National School Health Survey (PeNSE 2012).

    Science.gov (United States)

    Ferreira, Nathália Luíza; Claro, Rafael Moreira; Lopes, Aline Cristine Souza

    2015-12-01

    This study aimed to analyze the consumption of high-sugar foods by Brazilian schoolchildren and to identify associated factors, based on data from the National School Health Survey (PeNSE 2012). Consumption of these foods was classified as: do not consume sweets and soft drinks regularly; consume sweets or soft drinks regularly; and consume sweets and soft drinks regularly. Its association with sociodemographic information, eating habits, and family contexts were investigated via multiple ordinal regressions. Regular consumption of sweets and/or soft drinks was reported by 19.2% and 36.1% of adolescents, respectively, and higher prevalence was associated with female gender, age 14-15 years, higher maternal education, not living with the mother and father, not eating meals with the parents, eating while watching TV, and longer TV time. Nearly one-fifth of adolescents regularly consumed sweets and soft drinks, which was associated with socio-demographic and behavioral factors that should be targeted in order to improve their food consumption.

  1. Transfer Learning for SSVEP Electroencephalography Based Brain–Computer Interfaces Using Learn++.NSE and Mutual Information

    Directory of Open Access Journals (Sweden)

    Matthew Sybeldon

    2017-01-01

    Full Text Available Brain–Computer Interfaces (BCI using Steady-State Visual Evoked Potentials (SSVEP are sometimes used by injured patients seeking to use a computer. Canonical Correlation Analysis (CCA is seen as state-of-the-art for SSVEP BCI systems. However, this assumes that the user has full control over their covert attention, which may not be the case. This introduces high calibration requirements when using other machine learning techniques. These may be circumvented by using transfer learning to utilize data from other participants. This paper proposes a combination of ensemble learning via Learn++ for Nonstationary Environments (Learn++.NSEand similarity measures such as mutual information to identify ensembles of pre-existing data that result in higher classification. Results show that this approach performed worse than CCA in participants with typical SSVEP responses, but outperformed CCA in participants whose SSVEP responses violated CCA assumptions. This indicates that similarity measures and Learn++.NSE can introduce a transfer learning mechanism to bring SSVEP system accessibility to users unable to control their covert attention.

  2. Consumption of sugar-rich food products among Brazilian students:National School Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Nathália Luíza Ferreira

    2015-12-01

    Full Text Available Abstract This study aimed to analyze the consumption of high-sugar foods by Brazilian schoolchildren and to identify associated factors, based on data from the National School Health Survey (PeNSE 2012. Consumption of these foods was classified as: do not consume sweets and soft drinks regularly; consume sweets or soft drinks regularly; and consume sweets and soft drinks regularly. Its association with sociodemographic information, eating habits, and family contexts were investigated via multiple ordinal regressions. Regular consumption of sweets and/or soft drinks was reported by 19.2% and 36.1% of adolescents, respectively, and higher prevalence was associated with female gender, age 14-15 years, higher maternal education, not living with the mother and father, not eating meals with the parents, eating while watching TV, and longer TV time. Nearly one-fifth of adolescents regularly consumed sweets and soft drinks, which was associated with socio-demographic and behavioral factors that should be targeted in order to improve their food consumption.

  3. 18F-NaF PET-CT与血清肿瘤标志物联合检测对肺癌骨转移早期诊断的价值%Value of 18F-NaF PET-CT and serum tumor markers in the joint detection of bone metastases in recently diagnosed lung cancer patients

    Institute of Scientific and Technical Information of China (English)

    王明霜; 周凡; 甘西伦; 谭蓓蓓

    2015-01-01

    目的:探讨18F-NaF PET-CT与血清肿瘤标志物CEA、CYFRA21-1、NSE联合检测对肺癌骨转移患者早期诊断的临床意义。方法:选取46例来我院就诊,通过临床、CT、MRI等确诊为肺癌骨转移患者,分别于行18F-NaF PET-CT检查同时采取静脉血行血清CEA、CYFRA21-1、NSE检测,得到的最终诊断进行比较,分析两种检查联合检测在肺癌骨转移患者早期诊断中的临床意义。结果:46例患者18F-NaF PET-CT检查的SUVmax平均值为8.81±4.54,CEA平均值为37.45±42.57,CYFRA21-1平均值为32.30±42.66,NSE平均值为50.12±38.45。46例阳性患者,18F-NaF PET-CT检查出44例,灵敏度为93.40%,特异性为91.37%,准确性为93.50%;三种血清肿瘤标志物联合检测诊断肺癌的灵敏度分别为83.49%,特异性为70.52%,准确性为90.99%;两种检查联合诊断的灵敏度为96.85%,特异性为97.43%,准确性为98.33%。结论:18F-NaF PET-CT对肺癌骨转移早期患者诊断的灵敏度和准确性较血清CEA、CYFRA21-1、NSE检测高,但血清CEA、CYFRA21-1、NSE对其诊断也具有一定的价值,对前者检查可以起到协助补充的作用,两者联合应用对于肺癌骨转移早期患者的早期诊断具有重要的临床意义。%ObjectiveTo explore the joint detection value of 18F-NaF PET-CT and serum CEA,CYFRA21-1,and NSE in the diagnosis of bone metastases in recently diagnosed lung cancer patients. Methods46 cases of confirmed bone metastases of recently diagnosed lung cancer were selected, 18F-NaF PET-CT and serum CEA, CYFRA21-1,and NSE detection were performed at the same time, and their value in early detecting the bone metastasis was studied. Results In 46 cases, the SUV max of 18F-NaF PET-CT was 8.81 ± 4.54, serum CEA was 37.45±42.57,serum CYFRA21-1 was 32.30±42.66, seram NSE was 50.12±38.45. The sensitivity of 18F-NaF PET - CT examination was 93.40%, specificity was 91.37%, the diagnostic accuracy was 93

  4. Application of plasma NSE in moderate and severe craniocerebral trauma%血浆NSE测定在中重度颅脑外伤中的应用

    Institute of Scientific and Technical Information of China (English)

    林建虎; 何学雄; 陆川

    2011-01-01

    Objective: To explore the application of serum neuron specific enolase (NSE) on severity assessment and prognostic speculation of moderate and severe craniocerebral trauma. Methods: Ninety-six patients with moderate and severe TBI in our hospital from 2009. 5 to 2010.5 were selected. And those who have tumor,severe cardiopulmonary insufficiency and stroke, craniocerebral trauma, myocardial infarction recently, and injured longer than 6 hours were removed. Serum samples for NSE analysis were obtained from an antecubital vein 6 hours after TBI, and 1 day, 3 days, 5 days and 7 days later. Outcomes of all patients were evaluated with GOS 3 months after injury. Results: Ninety-six patients were divided into 2 groups according to GCS, moderate group (GCS 9~12) was 59 cases and severe group (GCS 3~8) 37 cases. And 40 health persons the corresponding time as control group. Serum NSE levels of moderate group and severe group were higher than that of control group, (P<0.01). Serum NSE levels of severe group were more higher than that of moderate group, P<0. 01. All patients went on 3-month follow up. Good prognosis (GOS 4~5) was found in 33 cases, poor prognosis (GOS 1~3) 63 cases. Serum NSE levels were significantly higher in the patients with poor prognosis than that in the patients with good prognosis (P<0. 01). Conclusion:Serum NSE content reflects the severity of crainiocerebral trauma, it is a sensitive quantitative index. Dynamic measurements of serum NSE contents after craniocerebral injury are of importance for clinical observation and outcome prediction.%目的:探讨神经元特异性烯醇化酶(NSE)在中重度颅脑损伤病情判断及预后预测中的应用.方法:选取我院2009年5月至2010年5月中重度颅脑外伤住院患者96例,按照入院GCS评分分为中度组59 例及重度组37例,设同期健康成人40例为对照组.抽取外伤后6 h、1 d、3 d、5 d、7 d的肘部静脉血,进行NSE测定.对96例患者进行为期3个月的

  5. Diagnostic value of combined detection of six kinds of tumor markers on lung cancer%6种肿瘤标志物联合检查对肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    贺望娇; 范柳青

    2014-01-01

    Objective To investigate the value of serum tumor markers neuron specific enolase (NSE),cytokeratin 19 fragment (CYFRA21-1),carcinoembryonic antigen (CEA),carbohydrate antigen 125 (CA125 ),carbohydrate antigen 153 (CA153)and fer-ritin in diagnosing lung cancer.Methods The serum levels of NSE,CYFRA21-1,CEA,CA125,CA153 and ferritin were detected by the electrochemiluminescence method in 151 cases of lung cancer(lung cancer group),100 cases of benign lung disease (benign dis-ease group)and 50 cases of healthy control group.The detection results were analyzed.Results The serum levels of NSE,CY-FRA21-1,CEA,CA125,CA153 and ferritin in the lung cancer group were significantly higher than those in the benign lung disease group and the healthy control group with statistical differences (P <0.05);the NSE level in small cell lung cancer was higher than that in squamous carcinoma and adenocarcinoma with statistical difference(P <0.05).The CYFRA21-1level in squamous carcinoma and adencarcinoma was higher than that in small cell lung cancer with statistical difference (P <0.05).The sensitivity of NSE was highest in six tumor markers,which was 82.8%.In 6 tumor markers,3 markers were positive,the positive rate of lung cancer was higher,while the false positive rate was significantly reduced.Conclusion The levels and positive rates of serum tumor markers are associated with the pathological type of lung cancer.The combined detection of serum tumor markers can increase the sensitivity and accuracy in early diagnosis of lung cancer.The combination measurement of CEA,NSE and CYFRA21-1 is the first choice.%目的:探讨肿瘤标志物神经特异性烯醇化酶(NSE)、细胞角蛋白(CYFRA21-1)、癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原153(CA153)和铁蛋白(Ferritin)对肺癌的诊断价值。方法采用电化学发光法分别检测151例肺癌(肺癌组)、100例肺良性疾病患者(肺良性疾病组)及50

  6. Plasma miR-182 expression in non-small cell lung cancer and its relationship with tumor CT findings and malignant biological molecule expression

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    Xue-Ping Zeng; Li Lu; Ting Huang; Qi-Cong Zhu

    2016-01-01

    Objective:To study the plasma miR-182 expression in non-small cell lung cancer and its relationship with tumor CT findings and malignant biological molecule expression.Methods:A total of 60 patients who were diagnosed with non-small cell lung cancer in our hospital from May 2012 to October 2015 were included in NSCLC group of the study, and 80 cases of healthy volunteers who received physical examination in our hospital during the same period and whose general data matched with that of NSCLC patients were included in control group of the study. Contrast-enhanced CT was conducted and the major diameter and minor diameter of lesions were measured, plasma was collected to determine miR-182 expression as well as CEA, CYFRA21-1, SCC-Ag and TSGF levels, and tumor tissue was collected to determine the content of RECK, MTSS1, PDCD4 and DNMT3a.Results: Relative plasma miR-182 expression of NSCLC group was significantly higher than that of control group; axial maximum major diameter, maximum minor diameter perpendicular to it and coronary maximum vertical major diameter in non-small cell lung cancer patients with high plasma miR-182 expression were significantly higher than those in non-small cell lung cancer patients with low plasma miR-182 expression, plasma CEA, CYFRA21-1, SCC-Ag and TSGF levels were significantly higher than those in non-small cell lung cancer patients with low plasma miR-182 expression, RECK, MTSS1, PDCD4 and DNMT3a levels in tumor tissue were significantly lower than those in non-small cell lung cancer patients with low plasma miR-182 expression, and differences in above indexes between two groups were statistically significant (P<0.05).Conclusions: Plasma miR-182 expression is abnormally high in non-small cell lung cancer and is closely related to the CT findings of tumor, the content of serum tumor markers and the expression of malignant molecules in tumor tissue.

  7. Diagnostic value of tumor markers in lung cancer%肿瘤标记物联合检测在肺癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    赵翔; 路平

    2009-01-01

    Objective To explore the diagnostic value of 3 tumor markers,Carcinoembryonic antigen(CEA),Cytokeratin fragment 21-1(CYFRA21-1)and Neuron-specific enolase(NSE),in different histological lung cancers.Methods Concentration of NSE,CEA and CYFRA21-1 were(8,80±1.63)μg/L,(83.30±3.62)μg/L,(6.90±0.68)μg/L in patients with adenocarcinoma;(8.60±1.47)μg/L,(14.30±3.25)μg/L,(8.30±1.03)μg/L in patients with squamous cell carcinoma and(33.80±4.24)μg/L,(13.60±1.95)μg/L,(34.80±5.64)μg/L in patients with undifferentiated small cell cancer.The concentration of these three tumor markers in lung cancer group were significantly higher than those in benign disease group[(7.80±1.58)μg/L、(7.90±1.12)μg/L、(1.86±1.53)μg/L,(P<0.01)].The sensitivity of CYFRA 21-1 in squamous cell carcinoma was 72.2%;CEA in adenocarcinoma was 69.8%:NSE in undifferentiated small cell cancer was 63.6%.The sensitivity and specificity of associated tumor markers were 72.9%and 91.4%.Conclusion CYFRA21-1.CEA and NSE are useful for diagnosis of lung cancer with different histological type,especially with combined detection of the 3 serum tumor markers.%目的 探讨癌胚抗原(CEA)、血清神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CY-FRA21-1)在肺癌中的临床诊断价值.方法 采用电化学发光免疫分析技术测定85例肺癌患者(肺癌组)、36例肺良性疾病患者(良性病变组)血清中的CEA、CYFRA21-1和NSE水平.结果 NSE、CEA、CYFRA21-1在肺腺癌患者血清中的浓度分别为(8.80±1.63)μg/L、(83.30±3.62)μg/L、(6.90±0.68)μg/L,在鳞癌中分别为(8.60±1.47)μg/L、(14.30±3.25)μg/L、(8.30±1.03)μg/L,在小细胞未分化癌中分别为(33.80±4.24)μg/L、(13.60±1.95)μg/L、(34.80±5.64)μg/L均高于良性病变组的(7.80±1.58)μg/L、(7.90±1.12)μg/L、(1.86±1.53)μg/L,差异有统计学意义(均P<0.01).CYFRA21-1在鳞癌的敏感度最高(72,2%);NSE在小细胞未分化癌敏感度最高(63.6%);CEA

  8. Factors associated with injuries in adolescents, from the National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To estimate the prevalence of injuries among teenagers and to examine the associated risk factors, such as sociodemographic characteristics, risk behaviors, family ties and other factors. METHOD: The prevalence of the outcome (injury was estimated with a 95%confidence interval. In order to verify factors associated with the injury, a bivariate analysis was made with estimated odds ratio (OR and its respective confidence intervals. Then, a multivariate analysis was carried out, only with variables whose descriptive level was equal to or lower than 5% (p < 0.05 remaining in the model. RESULTS: The study of injury in adolescents, based on the data from the National Adolescent School-based Health Survey (PeNSE, pointed out that 10.3% of the teenagers suffered severe injuries in the past 12 months, such as cuts or perforations, broken bones or dislocated joints. The following variables remained independently associated with "suffering severe injuries": being a male teenager; black, mulatto or indigenous race/color and working. Factors related to family ties are significant when the relations are fragile amongst members: adolescents that are injured the most are the ones who suffer most aggressions at home, who skip classes without notifying their parents, those who do not live with their parents and have low family control. The most relevant aspects of mental health are insomnia and loneliness. The factors associated to the exposure to situations of violence that remained in the model were: insecurity in school and in the route home-school; getting a ride with someone inebriated; drinking and driving motorized vehicles; not wearing the seatbelt; not wearing a helmet and being bullied. Among the factors of individual behavior, the following can be emphasized: use of alcohol, cigarettes, trying illicit drugs and early sexual intercourse. CONCLUSION: The analysis of the determinants for suffering injuries in childhood and adolescence shows

  9. The significance of combining detection tumor markers in early diagnosis of lung cancer%联合检测肿瘤标志物在肺癌早期诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    朱红; 黄祥磊; 蒋冉; 谭业颖

    2013-01-01

    目的:探讨神经元特异性烯醇化酶( NSE)细胞角蛋白19片段( CYF R A21-1)、CA199、CA1254种肿瘤标志物在肺癌诊断中的意义。方法:分别对80例肺癌患者、70例肺部良性患者、65例健康查体人员的血清NSE、CYF R A21-1、CA199、CA125水平进行化学发光法测定分析。并比较三组各指标的阳性率以及4项指标单一或联检的敏感度和特异度。结果:肺癌组NSE、CYF R A21-1、CA199、CA125水平明显高于肺部良性疾病组和健康对照组(P <0.01)。肺癌组各指标阳性率明显高于对照组以及良性肺病组(P <0.05)。结论:联合检测肺癌病人血清NSE、CYF R A21-1、CA199、CA125水平可提高肺癌早期诊断的阳性率。%Objective:To explore the clinical significance of serum tumor markers(neuron specific enolase ( NSE),cytokeratin 19 fragment ( CYFRA21- 1) CA199 and CA125)in diagnosis of lung cancer. Methods:Serum levels of NSE,CYFRA21- 1, CA199 and CA125 were detected by ECLIA in 80 patients with lung cancer,70 patients with benign lung diseases and 65 healthy controls.Positive rate of the four parameters were compared among the three groups,and individualand combined sensitivity and specificity of the six parameters were also compared.Results:The serum levels of NSE,CYFRA21-1,CA199 and CA125 in four patients with lung cancer were significantly higher than those of patients with benign lung diseases and control group ( P < 0.01) .The lung cancer group showed higher positive rate of these indicators than the other groups ( both P < 0.05). Conclusions:Combined detection of these tumor markers' levels can raise the sensitivity in diagnosis of early lung cancer.

  10. Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals

    Institute of Scientific and Technical Information of China (English)

    Faridah Yusof; Atheer Awad Mehde; Wesen Adel Mehdi; Raha Ahmed Raus; Hamid Ghazali; Azlina Abd Rahman

    2015-01-01

    Objective Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage. Methods Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study. Their blood urea, creatinine, protein levels and DNase I/II activity levels were measured by spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the government health clinics in Kuantan-Pahang and fulfilled the inclusion criteria. Results The result indicated that mean levels of sera NSMCE2 have a significantly increase (P Conclusion This study suggests that an increase in serum concentrations of DNase I/II and E3 SUMO-protein ligase NSE2 level can be used as indicators for the diagnosis of kidney injury in patients with nephrolithiasis.

  11. Efficiency of S&P CNX Nifty Index Option of the National Stock Exchange (NSE, India, using Box Spread Arbitrage Strategy

    Directory of Open Access Journals (Sweden)

    G. P. Girish

    2014-09-01

    Full Text Available Box spread is a trading strategy in which one simultaneously buys and sells options having the same underlying asset and time to expiration, but different exercise prices. This study examined the efficiency of European style S&P CNX Nifty Index options of National Stock Exchange, (NSE India by making use of high-frequency data on put and call options written on Nifty (Time-stamped transactions data for the time period between 1st January 2002 and 31st December 2005 using box-spread arbitrage strategy. The advantages of box-spreads include reduced joint hypothesis problem since there is no consideration of pricing model or market equilibrium, no consideration of inter-market non-synchronicity since trading box spreads involve only one market, computational simplicity with less chances of mis-specification error, estimation error and the fact that buying and selling box spreads more or less replicates risk-free lending and borrowing. One thousand three hundreds and fifty eight exercisable box-spreads were found for the time period considered of which 78 Box spreads were found to be profitable after incorporating transaction costs (32 profitable box spreads were identified for the year 2002, 19 in 2003, 14 in 2004 and 13 in 2005 The results of our study suggest that internal option market efficiency has improved over the years for S&P CNX Nifty Index options of NSE India.

  12. [Prevalence of risk health behavior among adolescents: results from the 2009 National Adolescent School-based Health Survey (PeNSE)].

    Science.gov (United States)

    Malta, Deborah Carvalho; Sardinha, Luciana Monteiro Vasconcelos; Mendes, Isabel; Barreto, Sandhi Maria; Giatti, Luana; Castro, Inês Rugani Ribeiro de; Moura, Lenildo de; Dias, Antonio José Ribeiro; Crespo, Claudio

    2010-10-01

    Adolescence is typically a developmental phase characterized by change and experimentation and therefore varying exposure to health hazards. We aimed at estimating the prevalence of protective and risk health behaviors of adolescents interviewed in the 2009 National Adolescent School-based Health Survey (PeNSE). PeNSE, a partnership between the Instituto Brasileiro de Geografia e Estatística (IBGE) and the Ministry of Health, using a PDA-based self-administered questionnaire collected information on 60,973 students enrolled at 1,453 public and private schools in the 27 state capitals and the Federal District. Among the food items most often consumed ( > or = 5 days per week), were beans (62.6%) and fruit (31.5%), but also sweets (58.3%) and soft drinks (37%), 43.1% of the students were sufficiently physically active but 79.5% spent >2 hours per day in front of the TV. As for drug use, 6.3% reported being current tobacco smokers, 27% drank alcoholic beverages regularly and 8.7% had used illicit drugs at least once. These results should guide stakeholders and policy makers in the development and implementation of programs and recommendations aimed at curtailing unhealthy exposures of adolescents in Brazil.

  13. Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals.

    Science.gov (United States)

    Yusof, Faridah; Mehde, Atheer Awad; Mehdi, Wesen Adel; Raus, Raha Ahmed; Ghazali, Hamid; Rahman, Azlina Abd

    2015-09-01

    Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage. Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study. Their blood urea, creatinine, protein levels and DNase I/II activity levels were measured by spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the government health clinics in Kuantan-Pahang and fulfilled the inclusion criteria. The result indicated that mean levels of sera NSMCE2 have a significantly increase (P<0.01) in patients compared to control group. Compared with control subjects, activities and specific activities of serum DNase I and II were significantly elevated in nephrolithiasis patients (P$lt;0.01). This study suggests that an increase in serum concentrations of DNase I/II and E3 SUMO-protein ligase NSE2 level can be used as indicators for the diagnosis of kidney injury in patients with nephrolithiasis. Copyright © 2015 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

  14. Brain injury markers (S100B and NSE in chronic cocaine dependents Marcadores de lesão cerebral (S100B e NSE em dependentes crônicos de cocaína

    Directory of Open Access Journals (Sweden)

    Felix Henrique Paim Kessler

    2007-06-01

    Full Text Available OBJECTIVE: Studies have shown signs of brain damage caused by different mechanisms in cocaine users. The serum neuron specific enolase and S100B protein are considered specific biochemical markers of neuronal and glial cell injury. This study aimed at comparing blood levels of S100B and NSE in chronic cocaine users and in volunteers who did not use cocaine or other illicit drugs. METHOD: Twenty subjects dependent on cocaine but not on alcohol or marijuana, and 20 non-substance using controls were recruited. Subjects were selected by consecutive and non-probabilistic sampling. Neuron specific enolase and S100B levels were determined by luminescence assay. RESULTS: Cocaine users had significantly higher scores than controls in all psychiatric dimensions of the SCL-90 and had cognitive deficits in the subtest cubes of WAIS and the word span. Mean serum S100B level was 0.09 ± 0.04 µg/l among cocaine users and 0.08 ± 0.04 µg/l among controls. Mean serum neuron specific enolase level was 9.7 ± 3.5 ng/l among cocaine users and 8.3 ± 2.6 ng/l among controls. CONCLUSIONS: In this first study using these specific brain damage markers in cocaine users, serum levels of S100B and neuron specific enolase were not statistically different between cocaine dependent subjects and controls.OBJETIVO: Estudos têm demonstrado sinais de lesão cerebral causadas por diferentes mecanismos em usuários de cocaína. A enolase sérica neurônio-específica e a proteína S100B são consideradas marcadores bioquímicos específicos de lesão neuronal e glial. Este estudo objetivou comparar os níveis sangüíneos de S100B e enolase sérica neurônio-específica em usuários crônicos de cocaína e em voluntários que não usam cocaína ou outras drogas ilícitas. MÉTODO: Vinte sujeitos dependentes de cocaína, mas não dependentes de álcool, maconha ou outra droga, e 20 sujeitos controles não usuários de drogas foram recrutados. Os sujeitos foram selecionados por

  15. 研究血清多种肿瘤标志物联合检测在肺癌诊断中的价值%Study of Serum Tumor Markers in Diagnosis of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    万程彬

    2014-01-01

    Objective: To investigate the serum tumor markers in diagnosis of lung cancer, find the most effective way for the diagnosis of lung cancer.Method: 200 patients with lung cancer from 2012 May to 2013 December were selected in our hospital for carcinoembryonic antigen (CEA), carbohydrate anticarcinogens 199 (CA19-9), carbohydrate antigen 125 (CA125 cancer), neuron specific enolase (NSE) and cytokeratin 19 (CYFRA21-1) sensitivity, accuracy and specificity of detection of 5 items. Serum carcinoembryonic antigen (CEA), cytokeratin 19 (CYFRA21-1), neuron specific enolase (NSE), carbohydrate anticarcinogens 199 (CA19-9) and carbohydrate antigen 125 (CA125) cancer were detected by chemiluminescence method, the result would be detected for statistical data processing.Result: The expression of cytokeratin 19 (CYFRA21-1) sensitivity and accuracy in the diagnosis of lung cancer in the detection of single item in the highest (64% and 75.6%), but the specificity was carbohydrate anticarcinogens 199 (CA19-9) to (98.8%) in the first row. The testing data and the single project relative sensitivity and accuracy of combined detection had significantly increased, the neuron specific enolase (NSE) and cytokeratin 19 (CYFRA21-1) of (83.6% and 82.9%) in the first row, while the carbohydrate specificity of cancer antigen 125 (CA125) and carbohydrate anticarcinogens 199 (CA19-9) (97.6%) the highest.Conclusion: The results show the sensitivity, accuracy of joint detection in the diagnosis of lung cancer have significantly improved the accuracy of diagnosis of lung cancer, the great, can be widely applied to clinical.%目的:研究血清多种肿瘤标志物联合检测在肺癌诊断中的价值,寻找最为有效的肺癌诊断方式。方法:选取自2012年5月-2013年12月入住本院的200例肺癌患者进行癌胚抗原(CEA)、糖类抗癌原199(CA19-9)、糖类癌抗原125(CA125)、神经元特异性烯醇化酶(NSE)和细胞角蛋白片段19(CYFRA21-1

  16. Diagnostic value of combination detection of CEA,CYFR21-1,NSE in bronchoalveolar lavage fluid for early lung cancer%肺泡灌洗液中 CEA 、CYFR21-1、NSE 联合检测对早期肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    陈元菁; 顾晔

    2016-01-01

    目的:探讨肺泡灌洗液(BALF)中癌胚抗原(CEA)、细胞角蛋白19片段(CYFR)21‐1、神经元特异性烯醇化酶(NSE)联合检测对早期肺癌的诊断价值,以及其与临床疗效的相关性。方法69例肺癌患者纳入肺癌组,50例肺部良性疾病患者纳入肺良性疾病组。所有患者行肺泡灌洗术(BAL),采用化学发光免疫法检测 BALF 中 CEA 、CYFR21‐1、NSE 水平,比较2组患者及不同临床疗效肺癌患者肿瘤标志物水平的变化。结果肺癌组 BALF 中 CEA 、CYFR21‐1、NSE 水平明显高于肺良性疾病组,差异有统计学意义(P<0.05)。Ⅱ期肺癌患者各肿瘤标志物水平均高于Ⅰ期患者,差异有统计学意义(P<0.05);随着临床疗效的下降,CEA 、CYFR21‐1、NSE 水平逐渐升高(P <0.05);联合检测的灵敏度和特异度分别为62.3%、82.0%,明显高于 CEA 、CY‐FR21‐1、NSE 任一单项的灵敏度和特异度(P<0.05)。结论早期肺癌患者 BALF 中 CEA 、CYFR21‐1、NSE 水平明显升高,且与肺癌病理分期密切相关,联合检测有助于提高早期肺癌检出率及指导临床疗效评估。%Objective To explore the diagnostic value of combination detection of carcino‐embryonic antigen(CEA ) ,cytokeratin 19 fragment 21‐1(CYFR21‐1) ,neuron‐specific enolase(NSE) in bronchoalveolar lavage fluid (BALF) for early lung cancer and its correlation with clinical effects .Methods 69 cases of lung cancer were included into the lung cancer group and 50 cases of benign pulmonary disease were included into the lung benign disease group .All the patients were given bronchoalveolar lavage(BAL) .The chemiluminescence immunoassay was adopted to detect the levels of CEA ,CYFR21‐1 and NSE in BALF .The changes of tumor markers levels were compared between the two groups and among different clinical curative effects in the patients with lung cancer . Results The

  17. Comparison of plasma NSE, protein S-100b and EEG changes in traditional arrested-heart procedures and on-pump beating-heart procedures

    Institute of Scientific and Technical Information of China (English)

    王咏; 肖颖彬; 陈林; 王学锋; 钟前进

    2003-01-01

    Objective: To assess the cerebral injury in on-pump beating-heart procedures under mild hypothermia in comparison with traditional on-pump arrested-heart procedures under moderate hypothermia. Methods: Forty patients, 20 with congenital heart disease (CHD) and 20 of rheumatic heart disease (RHD), were divided into 2 groups: Control group (group A, n=20) including 10 patients suffering from CHD as group A1 and the left 10 from RHD as group A2; and experiment group (group B, n=20) which consisting of group B1 (10 with CHD) and group B2 (10 of RHD). The patients in group A underwent traditional arrested-heart procedures, and those in group B were operated on with beating-heart procedures. Arterial blood samples were collected at preoperation (time A), 20 min after cardiopulmonary bypass (CPB) starting (time B), 1 h after CPB (time C) and 24 h postoperation (time D) respectively. Plasma contents of neuron-specific enolase (NSE) and protein S-100b were measured with sensitive ELISA. All the patients received echoencephalography (EEG) before and 1 week after operation. Results: The plasma contents of protein S-100b were increased very significantly at time B, C and D in comparison with those at time A (P<0.01), and that of patients in group A1 was significantly higher than that in group B at time B (P<0.05). There was no significant difference at other time points. At time B, the plasma contents of NSE were significantly higher in group A than in group B, and in group A1 and B1 than in group A2 and B2. What's more, at time B, the former fell back to their preoperative levels, but the latter remained still higher levels than the preoperative ones (P<0.01). No significant difference was found in the abnormality rates of postoperative EEG between 2 groups. Conclusion: The perioperative plasma contents of NSE and protein S-100b are not significantly higher in group B than in group A. On-pump beating-heart procedures do not make more serious cerebral dysfunction than the

  18. An analysis of control plane updates for NPU and NSE based systems%基于NPU和NSE系统的控制平面分析

    Institute of Scientific and Technical Information of China (English)

    Karthik Ranganathan; Jagadeesan Rajamanickam

    2004-01-01

    典型的网络系统包括一个或多个用于监视数据平面分组处理的控制平面CPU。通过CPU进行的控制更新将导致存储于网络搜索引擎(NSE)和相联SRAM中的路由表和政策表的更新。在数据平面中,NSE和SRAM通过诸如网络处理机论坛的LookAside-1(LA-1)接口等高速接口连接至网络处理单元(NPU)。从外部CPU至NSE和SRAM的控制平面更新信息可以经由带内线路(即在NPU内部并通过NPU的LA-1接口)来传送。

  19. Application of Tumor Markers in the Diagnosis of Lung Cancer%肿瘤标志物在肺癌辅助诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    韩宾

    2016-01-01

    Objective Lung cancer tumor markers in lung cancer diagnosis and assessment of recurrence, surgery has a higher value,.This study of 5 kinds of serum tumor markers in diagnosis of lung cancer application, including the neuron-specific enolase (NSE) and carcinoembryonic Antigen (CEA) and cancer Antigen 125 (CA125) and Cytokeratin 19 fragment () and squamous cell carcinoma Antigen (SCC);Methods 120 cases of patients with lung cancer, 80 healthy and Sera of 98 patients with benign disease.Above 5 kinds of markers are made of Elisa experiments (ELISA) testing. Results 5 kinds of serum tumor markers in patients with lung cancer are significantly higher than the non-tumor group and healthy groups and benign disease group, a statistically significant difference ( <0.01). NSE in small cell lung cancer, CYFRA21-1 and squamous cell carcinoma Antigen (SCC) Squamous cell carcinomas were significantly higher than those in other types of lung cancer ( <0.01);Detection sensitivity of NSE for small cell lung cancer was significantly higher than other types of lung cancer ( <0.01);More than 5 kinds of serum tumor markers combination, sensitivity was significantly higher than any single cancer markers ( <0.01).Conclusion 5 kinds of serum tumor markers for lung cancer diagnosis has clinical significance. NSE can be used as a marker for detection of small cell lung cancer;CEA +CYFRA21-1+SCC can be used to inspect non-small cell lung cancer with combination of markers. CYFRA21-1+SCC can be used to inspect combinations of markers in lung squamous cell carcinoma.%目的:肺癌肿瘤标志物在肺癌诊断、复发、手术评估方面均有较高价值,本研究探讨5种血清肿瘤标志物神经原特异性烯醇化酶(NSE)、癌胚抗原(CEA)、癌抗原125(CA125)、细胞角蛋白19片段(CYFRA21-1)和鳞状细胞癌抗原(SCC)在肺癌辅助诊断中的应用价值,及敏感性和特异性最高的肿瘤标志物组合。方法收集120例肺癌患者,80例健康人和98例肺

  20. Acute sleep deprivation increases serum levels of neuron-specific enolase (NSE) and S100 calcium binding protein B (S-100B) in healthy young men.

    Science.gov (United States)

    Benedict, Christian; Cedernaes, Jonathan; Giedraitis, Vilmantas; Nilsson, Emil K; Hogenkamp, Pleunie S; Vågesjö, Evelina; Massena, Sara; Pettersson, Ulrika; Christoffersson, Gustaf; Phillipson, Mia; Broman, Jan-Erik; Lannfelt, Lars; Zetterberg, Henrik; Schiöth, Helgi B

    2014-01-01

    To investigate whether total sleep deprivation (TSD) affects circulating concentrations of neuron-specific enolase (NSE) and S100 calcium binding protein B (S-100B) in humans. These factors are usually found in the cytoplasm of neurons and glia cells. Increasing concentrations of these factors in blood may be therefore indicative for either neuronal damage, impaired blood brain barrier function, or both. In addition, amyloid β (Aβ) peptides 1-42 and 1-40 were measured in plasma to calculate their ratio. A reduced plasma ratio of Aβ peptides 1-42 to 1-40 is considered an indirect measure of increased deposition of Aβ 1-42 peptide in the brain. Subjects participated in two conditions (including either 8-h of nocturnal sleep [22:30-06:30] or TSD). Fasting blood samples were drawn before and after sleep interventions (19:30 and 07:30, respectively). Sleep laboratory. 15 healthy young men. TSD increased morning serum levels of NSE (P = 0.002) and S-100B (P = 0.02) by approximately 20%, compared with values obtained after a night of sleep. In contrast, the ratio of Aβ peptides 1-42 to 1-40 did not differ between the sleep interventions. Future studies in which both serum and cerebrospinal fluid are sampled after sleep loss should elucidate whether the increase in serum neuron-specific enolase and S100 calcium binding protein B is primarily caused by neuronal damage, impaired blood brain barrier function, or is just a consequence of increased gene expression in non-neuronal cells, such as leukocytes.

  1. Increased serum midkine concentration as a possible tumor marker in patients with superficial esophageal cancer.

    Science.gov (United States)

    Shimada, Hideaki; Nabeya, Yoshihiro; Okazumi, Shin-ichi; Matsubara, Hisahiro; Kadomatsu, Kenji; Muramatsu, Takashi; Ikematsu, Shinya; Sakuma, Sadatoshi; Ochiai, Takenori

    2003-01-01

    Midkine, a heparin-binding growth factor, is expressed in numerous cancer tissues and is reportedly elevated in patients with various neoplasms. The aim of this study was to evaluate the clinicopathological significance of serum midkine concentration (S-MK) in patients with superficial esophageal squamous cell carcinoma (SCC). Pretreatment S-MK was measured by enzyme-linked immunosorbent assay in 135 healthy controls, 16 patients with benign esophageal disease, and 60 patients with primary superficial esophageal squamous cell cancer (SESCC). All patients with SESCC underwent curative resection. The disease was staged according to TNM/UICC guidelines. Serum concentrations of carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag), and cytokeratin 19 fragment (CYFRA21-1) were also evaluated in the same populations. S-MK in patients with SESCC (388+/-411 pg/ml) was significantly higher than in benign esophageal disease or healthy controls (183+/-73 and 154+/-76 pg/ml, respectively). Using the mean + 2 standard deviations of healthy control S-MK (300 pg/ml) as the cut-off level, 50% of patients with esophageal SESCC were deemed positive. This S-MK positivity rate for detecting SESCC was significantly higher than for other tumor markers. Thus, S-MK may be useful as a tumor marker to detect SESCC.

  2. Study on the Clinical Value of Pulmonary Tumor Markers and Bone Alkafine Phosphatase Detection in Early Diagnosis of Bone Metastasis of Lung Cancer%肺肿瘤标志物及碱性磷酸酶对肺癌骨转移早期诊断的临床意义

    Institute of Scientific and Technical Information of China (English)

    李殿波; 姜格宁

    2015-01-01

    Objective To study the clinical significance of pulmonary tumormarkers:cytokeratin19 solu-ble fragments ( CYFRA21-1 ) , carcinoembryonic antigen ( CEA ) , neuron specific enolase ( NSE ) , carbohy-drate antigen 125(CA125) and bone alkaline phosphatase(BALP) in early diagnosis of bone metastasis of lung cancer.Methods A total of 123 patients hospitalized in Linyi Tumor Hospital from Jul.2009 to Dec. 2011[including 47 cases of lung benign tumor(as the benign lung tumor group),40 cases of lung cancer without bone metastases(as the lung cancer without bone metastases group),and 36 cases of lung cancer with bone metastases( as the bone metastasis of lung cancer group ) ] were selected.The enzyme-linked immu-nosorbent assay was applied to detect the expression levels of CYFRA21-1,CEA,NSE,CA125 and BALP in the serum of all the patients,and such levels were compared among the groups.Results The expression lev-els of CYFRA 21-1,CEA,NSE,CA125,BALP of benign lung tumor group were (5.0 ±0.8) μg/L,(6.7 ± 0.5) pg/L,(18.9 ±2.5)μg/L,(29.0 ±2.8) kU/L,( 224.7 ±16.5) U/L;those of the lung cancer with-out bone metastases group were (15.1 ±2.7) μg/L,(10.6 ±1.7) pg/L,(30.2 ±4.2) μg/L,(60.1 ± 4.7) kU/L,(454.6 ±32.7) U/L;and those of the bone metastasis of lung cancer group were (29.7 ± 8.8) μg/L, (18.2 ±1.8) pg/L,(58.2 ±6.9) μg/L,(100.7 ±8.8) kU/L, (668.2 ±45.8) U/L.Such levels of the lung cancer without bone metastases group and bone metastasis of lung cancer group significantly increased compared with benign lung tumor group ( P <0.05 ).Such levels of the bone-metastasis of lung cancer group were also significantly higher than those of the lung cancer without bone metastases group ( P<0.05).Conclusion Detection of serum levels of CYFRA21-1,CEA,NSE,CA125 and BALP in patients with bone metastases of lung cancer can reveal the biological changes of such patients and has certain signifi-cance in the early diagnosis of bone-metastasis of lung cancer.%目的:探讨肺

  3. Study the effect of kidney stones on serum xanthine oxidase, ecto-5ʹ-nucleotidase activity and E3 SUMO-protein ligase NSE2 (NSMCE2 in Malaysian individuals

    Directory of Open Access Journals (Sweden)

    Faridah Yusof

    2015-08-01

    Conclusions: The present study suggests that the increase in serum of xanthine oxidase,ecto-5ʹ-nucleotidase activities E3 small ubiquitin-like modifier-protein ligase NSE2 concentration can be used as biomarkers for diagnosis of kidney damage in patients with kidney stone, also in developments of change DNA damage and inflammation disorders in these patients.

  4. 肺癌组织中神经元特异性烯醇化酶和总RNA的表达与改变%EXPRESSION AND ALTERATION OF NEURON-SPECIFIC ENOLASE (NSE) AND TOTAL RNA IN LUNG CANCER TISSUES

    Institute of Scientific and Technical Information of China (English)

    曹飞; 吴玮; 姚登福; 邱历伟; 陆建新

    2001-01-01

    目的:探讨神经元特异性烯醇化酶(NSE)在肺癌中的表达及其诊断价值。 方法:收集人肺癌,癌周及远癌组织各30份,制备和定量总RNA水平,并在 肺癌组织匀浆后,分析组织中NSE的浓度变化。结果:肺癌组织NSE表达显 著高于癌周及远癌组织(P<0.01 ),并与总RNA浓度成正相关(r[ WTBZ]=0.04,P<0.01),各种肺癌组织中NSE及RNA浓度无显著性差异。结论:提示肺癌组织核酸代谢旺盛,分析过度表达的NSE有助于肺癌的早期诊断 。%Objective: To explore the expression and diagnostic value of neuron- specific enolase (NSE) in patients with lung cancer. Methods: Human lung cancerous, paracancerous and distant tissues were collected, homogenates we re prepared, total RNAs were purified. The concerntrations of NSE and total RNA in tissues of 30 patients with lung cancer were investigated in this study. Results: The levels of NSE and total RNA in lung cancerous tissues were significantly higher (P<0.001) than those in paracancerous and in di stant tissues. The levels of NSE expression was positively correlated with lung to tal RNA concerntration (P<0.01). Conclusions: The data s uggest that nucleic acid metabolism is active in lung cancer tissues. Analysis o f excessive expression of NSE is useful for diagnosing and distinguishing lun g cancer.

  5. The serum levels of tumor marker CA19-9, CEA, CA72-4, and NSE in type 2 diabetes without malignancy and the relations to the metabolic control

    Science.gov (United States)

    Shang, Xiaojing; Song, Chunqing; Du, Xiaoming; Shao, Hailin; Xu, Donghong; Wang, Xiaolai

    2017-01-01

    Objectives: To investigate whether there is a difference in carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA), carbohydrate antigen 72-4 (CA72-4), and neuron-specific enolase (NSE) between diabetic and non-diabetic patients. Methods: A retrospective analysis was performed in 268 type 2 diabetic patients and 95 non-diabetic ones, and their serum levels of CA19-9, CEA, CA72-4, and NSE were compared in our endocrine ward at the Tianjin Fourth Central Hospital, Tianjin, China during the period from January to June 2015. The diabetic patients were divided into 4 groups based on glycosylated hemoglobin (HbA1c) levels to investigate the relationship between levels of tumor markers and glucose status. Results: Diabetic patients had higher levels of tumor markers than non-diabetic subjects (CA19-9: 13.0 versus 7.25U/mL, p=0.000; CEA: 2.55 versus 2.25 ng/mL, p=0.012; CA72-4: 1.95 versus 1.50U/mL, p=0.001; NSE: 11.64 versus 10.22ng/mL, p=0.000). CA19-9 levels increased in a stepwise manner with poor diabetes status. CEA levels were increased in patients with HbA1c ≥9% and CA72-4 elevation was predominant in patients with poor glycemic control (HbA1c ≥11%). NSE levels were not associated with metabolic parameters. Conclusion: Serum levels of CA19-9, CEA, CA72-4, and NSE were elevated in type 2 diabetes; however, only CA19-9, CEA, and CA72-4 levels were associated with hyperglycemia. PMID:28133696

  6. The Relationships of Emergence Delirium and S100β, NSE%全麻苏醒期谵妄与S100β、NSE的关系研究

    Institute of Scientific and Technical Information of China (English)

    刘卫华; 杜良琴; 王庭阔; 杨经文; 李恒

    2012-01-01

    目的:观察全麻患者苏醒期谵妄(Emergency Delirium,ED)与血浆S100β、NSE的关系.方法:选取84例择期全麻患者病人,于术前一天和术后苏醒期采用CAM量表进行谵妄状态评定,术后发生谵妄的患者为谵妄组,未发生谵妄的患者为对照组.并分别于术前、苏醒期,随机选择谵妄组42例,对照组42例抽取血清用ELASA方法测定S100β、NSE值.结果:血清S100β蛋白、NSE值在苏醒期谵妄患者和非谵妄组患者间差异无统计学意义(P>0.05).结论:血清S100β蛋白、血清NSE与苏醒期谵妄发生无明显相关性.%Objective: To observe the relationships between emergence delirium and S100β, NSE. Methods: 84 surgery patients under general anesthesia were included in this study. The confusion assessment method scale was employed to diagnose the occurrence of emergency delirium preoperative and postoperative. Then, the patients were divided into emergence delirium group and non-emergence delirium group based on the CAM scale. The levels of S100β, NSE were determinated by ELASA. Results: There was no significant difference in levels of S100β and NSE between emergence delirium group and non-emergence delirium group((P>0.05). Conclusion: The incidence of emergence delirium may be not associated with S100β and NSE.

  7. Inconsistent reports of risk behavior among Brazilian middle school students: National School Based Survey of Adolescent Health (PeNSE 2009/2012).

    Science.gov (United States)

    Ramos, Dandara de Oliveira; Daly, Martin; Seidl-de-Moura, Maria Lucia; Jomar, Rafael Tavares; Nadanovsky, Paulo

    2017-05-18

    This study assessed the consistency of self-reports of risk behavior (overall and within four specific domains: alcohol use, tobacco use, drug use, and sexual activity) in two editions of the Brazilian National School Based Survey of Adolescent Health (PeNSE): 2009 and 2012. The overall proportion of cases with at least one inconsistent response in the two editions was 11.7% (2.7% on the alcohol items, 2.1% for drug use, 4.3% for cigarette use, 3% for sexual activity) and 22.7% (12.8% on alcohol items, 2.5% for drug use, 4.3% for cigarette use, 4.1% for sexual activity), respectively. Such inconsistency was more prevalent among males, delayed students, those who reported having experimented with drugs, and those who did not have a cellphone. Because inconsistent responses were more prevalent among the students who claimed to have engaged in risky activities, removing inconsistent responders affected the estimated prevalence of all risk behaviors in both editions of the survey. This study supports the importance of performing consistency checks of self-report surveys, following the growing body of literature on this topic.

  8. Inconsistent reports of risk behavior among Brazilian middle school students: National School Based Survey of Adolescent Health (PeNSE 2009/2012

    Directory of Open Access Journals (Sweden)

    Dandara de Oliveira Ramos

    Full Text Available Abstract: This study assessed the consistency of self-reports of risk behavior (overall and within four specific domains: alcohol use, tobacco use, drug use, and sexual activity in two editions of the Brazilian National School Based Survey of Adolescent Health (PeNSE: 2009 and 2012. The overall proportion of cases with at least one inconsistent response in the two editions was 11.7% (2.7% on the alcohol items, 2.1% for drug use, 4.3% for cigarette use, 3% for sexual activity and 22.7% (12.8% on alcohol items, 2.5% for drug use, 4.3% for cigarette use, 4.1% for sexual activity, respectively. Such inconsistency was more prevalent among males, delayed students, those who reported having experimented with drugs, and those who did not have a cellphone. Because inconsistent responses were more prevalent among the students who claimed to have engaged in risky activities, removing inconsistent responders affected the estimated prevalence of all risk behaviors in both editions of the survey. This study supports the importance of performing consistency checks of self-report surveys, following the growing body of literature on this topic.

  9. Brain in human nutrition and variant Creutzfeldt-Jakob disease risk (vCJD): detection of brain in retail liver sausages using cholesterol and neuron specific enolase (NSE) as markers.

    Science.gov (United States)

    Lücker, E; Horlacher, S; Eigenbrodt, E

    2001-08-01

    No information is available about the consumption of brain via meat products. With respect to the new variant of Creutzfeldt-Jakob disease (vCJD) and the presumed food-borne transmission of bovine spongiform encephalopathy (BSE) to humans, a preliminary survey for brain and/or spinal cord (tissues of the central nervous system, CNS) was conducted. We applied a previously developed integrated procedure using cholesterol and neuron specific enolase (NSE) as markers. Quantification of cholesterol had to be backed up by NSE immunochemistry in order to account for low specificity and relatively high variances. Out of 126 high-quality finely graded liver sausages, five samples (4 %) showed positive NSE immunoresponses. In four of these samples a transgression of the normal maximum cholesterol content was obtained. The identification of such a considerable number of CNS-positive sausages indicates that brain consumption is not as rare as previously assumed. Overall, the present integrated method could be successfully applied for the detection of CNS in heat-treated meat products. Its routine application in official food control would deter illegal practice and thus help to control transmissible spongiform encephalopathies.

  10. Value of tumor markers to the diagnosis and therapeutic evaluation of lung CanCer%血清肿瘤标志物检测在肺癌诊断及疗效评估中价值

    Institute of Scientific and Technical Information of China (English)

    周超; 胡斌; 俞江月; 沈美珠; 王岸; 谷亮

    2011-01-01

    目的:评价血清肿瘤标志物在肺癌诊断及疗效判断中的应用价值.方法:检测105例肺癌(肺癌组)和24例肺部非肿瘤性疾病(非肺癌组)患者治疗前、后血清癌胚抗原(carcinoembryonic antigen,CEA)、神经元特异性烯醇化酶(neurone-specfic enolase,NSE)、细胞角质蛋白19片段(cytokeratin 19 fragment antigen 21-1,CYFRA21-1)水平,并进行分析和评价.结果:肺癌患者血清CEA,NSE,CYFRA21-1水平明显高于肺部非肿瘤性疾病患者,其中肺腺癌患者血清CEA水平最高,小细胞癌患者血清NSE水平最高,鳞癌患者血清CYFRA21-1水平最高;在化疗有效与无效者之间,血清肿瘤标志物水平差异有统计学意义(P<0.05).结论:肺癌患者血清CEA,NSE,CYFRA21-1测定有助于肿瘤细胞类型的判断,化疗前、后血清水平的变化与治疗效果密切相关.%Objective To evaluate the value of tumor markers to the diagnosis therapeutic evaluation of lung cancer.Methods A total of 105 patients with lung cancer and 24 patients with benign lung diseases were determined, analyzed and evaluated the serum levels of carcinoembryonic antigen, neurone-specific enolase and cytokeratin 19 fragment antigen 21-1.Results The carcinoembryonic antigen, neurone-specific enolase and cytokeratin 19 fragment antigen 21-1 levels were higher in lung cancer patients than those in patients with benign disease.The carcinoembryonic antigen level was the highest in adenocarcinoma patients, the neurone-specific enolase level was the highest in small cell lung cancer patients and cytokeratin 19 fragment antigen 21-1 level was the highest in squamous cell carcinoma patients.There was significant difference in the levels of tumor markers between the patients sensitive and nonsensitive to chemotherapy(P<0.05).Conclusion The determination of carcinoembryonic antigen, neurone-specific enolase and cytokeratin 19 fragment antigen 21-1 helps to judge the types of tumor cell.The serum change after

  11. DIAGNOSTIC VALUE OF SERUM VEGF, CEA AND NSE IN DIAGNOSIS OF NON-SMALL CELL LUNG CANCER%血清VEGF、CEA、NSE在非小细胞肺癌诊断中应用价值研究

    Institute of Scientific and Technical Information of China (English)

    郑立平; 林海峰

    2011-01-01

    [目的]探讨血清血管内皮生长因子(VEGF)、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)在非小细胞肺癌(NSCLC)诊断中的应用价值,旨在为肺癌的诊治提供理论参考.[方法]采用ELISA方法测定100例NSCLC患者和50例健康查体者血清VEGF、CEA、NSE水平,并分析其与病理分型和临床分期的关系.[结果]肺癌组血清CEA、NSE、VEGF水平均显著高于对照组,相比较差异有统计学意义(P<0.05);鳞癌患者血清CEA和NSE水平显著高于腺癌,相比较差异有统计学意义(P<0.05);而血清VEGF水平在鳞癌和腺癌患者中差异无统计学意义(P>0.05);血清VEGF、CEA、NSE水平随着临床分期的递增而显著上升,其血清水平为Ⅳ期>Ⅲ期>I~Ⅱ期,3组之间比较差异有统计学意义(P<0.05).[结论]联合检测肺癌患者血清中VEGF、CEA、NSE水平的变化,对NSCLC的早期诊断和鉴别具有重要的临床价值.%[Objective] To explore diagnostic value of serum vascular endotbelial growth factor (VEGF), earcinoembry-onic antigen (CEA) and neuron-specific enolase (NSE) for diagnosis of the non-small cell lung cancer (NSCLC), and to provide theoretical reference for diagnosis and treatment of NSCLC. [Methods] 100 cases of patients with NSCLC and 50 cases of healthy people were determined by ELJSA, and their relationship with pathological type and clinical stage were analyzed. [ Results ] The serum CEA, NSE and VEGF levels of the lung cancer group were significantly higher than that of control group, the difference was significant (P 0.05); The serum VEGF, CEA and NSE levels significantly increased with increasing clinical stage, the difference was significant among the three groups (P< 0.05). [Conclusion] Combined detection of the serum VEGF, CEA and NSE levels has important clinical value for the early diagnosis and diagnosis of NSCLC.

  12. The combination of exercise training and alpha-lipoic acid treatment has therapeutic effects on the pathogenic phenotypes of Alzheimer's disease in NSE/APPsw-transgenic mice.

    Science.gov (United States)

    Cho, Joon Y; Um, Hyun S; Kang, Eun B; Cho, In H; Kim, Chul H; Cho, Jung S; Hwang, Dae Y

    2010-03-01

    Exercise training was suggested as a practical therapeutic strategy for human subjects suffering from Alzheimer's disease (AD) in our previous study. Therefore, the purpose of this study was to investigate the effects of combining exercise training with the administration of antioxidants on the pathological phenotype of AD. To accomplish this, non-transgenic mice (Non-Tg) and NSE/APPsw Tg mice were treated with alpha-lipoic acid and treadmill exercised for 16 weeks, after which their brains were evaluated to determine whether any changes in the pathological phenotype-related factors occurred. The results indicated that (i) the combination-applied (COMA) Tg group with exercise training (ET) and alpha-lipoic acid administration (LA) showed ameliorated spatial learning and memory compared to the sedentary (SED)-Tg and single-treatment groups; (ii) there were no differences in the level of Abeta-42 peptides across groups; (iii) the level of glucose transporter-1 and brain-derived neurotrophic factor proteins were highly increased in the COMA group, (iv) ET and LA did not induce a synergistic effect on the expression of heat shock protein-70 and apoptotic proteins including Bax and caspase-3; (v) the levels of SOD-1 and CAT suppressing oxidative stress were extensively higher in the COMA than in the single-treated groups and (vi) there were no significant differences across groups regarding these serum characteristics, although these levels were lower than the SED-Tg group. Taken together, these results suggest that the combination with ET and LA may contribute to protect the neuron injury induced by Abeta peptides and may be considered an effective therapeutic strategy for human subjects suffering from AD.

  13. Lifetime use of illicit drugs and associated factors among Brazilian schoolchildren, National Adolescent School-based Health Survey (PeNSE 2012

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    Rogério Lessa Horta

    2014-01-01

    Full Text Available OBJECTIVE: This study aimed at describing the prevalence of illicit drug use among 9th grade students in the morning period of public and private schools in Brazil, and assessing associated factors. METHOD: The Brazilian survey PeNSE (National Adolescent School-based Health Survey 2012 evaluated a representative sample of 9th grade students in the morning period, in Brazil and its five regions. The use of illicit drugs at least once in life was assessed for the most commonly used drugs, such as marijuana, cocaine, crack, solvent-based glue, general ether-based inhalants, ecstasy and oxy. Data were subjected to descriptive analysis, and Pearson's χ2 test and logistic regression was used in the multivariate analysis. RESULTS: The use of illicit drugs at least once in life was reported by 7.3% (95%CI 5.3 - 9.4 of the respondents. Logistic regression was used for multivariate analysis and the evidences suggest that illicit drug use is associated to social conditions of greater consumption power, the use of alcohol and tobacco, behaviors related to socialization, such as having friends or sexual activity, and also the perception of loneliness, loose contact between school and parents and experiences of abuse in the family environment. The outcome was inversely associated with close contact with parents and parental supervision. CONCLUSION: In addition to the association with the processes of socialization and consumption, the influence of family and school is expressed in a particularly protective manner in different records of direct supervision and care.

  14. proGRP、NSE在小细胞肺癌鉴别诊断中的意义%Significance of proGRP and NSE in Differential Diagnosis of Small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    尹颜军; 荣长利; 郑华

    2013-01-01

    Objective proGRP and NSE are very important SCLC tumor markets, they are used not only in early screening, but also in diagnosis and differential diagnosis of benign or malignant pulmonary diseases. The aim of this study is to evaluate the value of two marker in diagnosis of SCLC and differential diagnosis. Methods Retrospectively analyze proGRP and NSE serum levels in 298 cases of SCLC, 368 eases of NSCLC and some benign disease patients. All data were analyzed by SPSS 13.0 and ROC was generated by SAS 9.0. Resuits proGRP and NSE are all significantly elevated in SCLC than in other pathological type, tuberculosis and pneumonia. Sensitivity and specificity of proGBF are 67.1% and 98.5% , respectively. Sensitivity and specificity of NSE are 60.1% and 89.3% , individually. ROC area under curve of proGRP, NSE and both are 0.845, 0.810 and 0.894, separately. Conclusion proGRF and NSE are all very useful tumor marker far SCLC and combination of the two markers in diagnosis and differential diagnosis will be more valuable.%目的:胃泌素释放肽前体(pro-gastrin-releasing peptide,proGRP)和神经特异性烯醇化酶(neuron specific enolase,NSE)均是小细胞肺癌的重要肿瘤标志物,不仅可用于早期筛查,对于各种良恶性肺部疾病的鉴别诊断也起到重要作用.本文拟评估两者在小细胞肺癌诊断和鉴别诊断中的价值.方法:回顾性分析298例SCLC与368例NSCLC以及肺部良性疾病中两者的水平,SPSS13.0进行统计分析,SAS 9.0进行ROC曲线绘制.结果:无论是proGRP还是NSE均在小细胞肺癌中明显升高,与其他病理类型肺癌或结核、肺炎相比均呈明显差异,proGRP的敏感性为67.1%,特异性为98.5%,NSE敏感性为60.1%,特异性为89.3%.proGRP、NSE及两者联合诊断SCLC的ROC曲线下面积分别为0.845、0.810、0.894.结论:proGRP和NSE均为SCLC诊断及鉴别诊断中非常好的标志物,两者联合诊断更加具有价值.

  15. Dor dentária e fatores associados em adolescentes brasileiros: a Pesquisa Nacional de Saúde do Escolar (PeNSE, Brasil, 2009 Dental pain and associated factors in Brazilian adolescents: the National School-Based Health Survey (PeNSE, Brazil, 2009

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    Maria do Carmo Matias Freire

    2012-01-01

    Full Text Available O objetivo deste estudo foi estimar a prevalência da dor de dente em adolescentes brasileiros e analisar fatores sociodemográficos e comportamentais associados, utilizando os dados da Pesquisa Nacional de Saúde do Escolar (PeNSE de 2009. A pesquisa foi realizada pelo Instituto Brasileiro de Geografia e Estatística e pelo Ministério da Saúde em escolares com idades entre 11 e 17 anos ou mais, das 27 capitais brasileiras, por meio de questionário autoaplicável. Utilizou-se a análise de regressão de Poisson, segundo um modelo hierárquico de determinação. A prevalência de dor na amostra (n = 54.985 nos últimos seis meses foi de 17,8% (IC95%: 17,5-18,1. Prevalências mais elevadas foram encontradas em mulheres, naqueles com 14 anos ou mais, das raças preta, parda e indígena, de escolas públicas, cujas mães tinham baixa escolaridade, que não moravam com a mãe, que haviam experimentado cigarro e álcool alguma vez na vida, que relataram menor frequência de escovação e maior consumo de guloseimas e refrigerantes. A prevalência de dor foi considerável e associada a aspectos sociodemográficos e de comportamentos relacionados à saúde.The aim of this study was to assess the prevalence of dental pain and associated socio-demographic and behavioral factors in Brazilian adolescents, using data from the National School-Based Health Survey (PeNSE, Brazil, 2009. The survey was conducted by the Brazilian Institute of Geography and Statistics (IBGE and Ministry of Health in students 11 to 17 years of age or older in the 27 State capitals, using a self-administered questionnaire. Analyses included Poisson regression following a hierarchical approach. Prevalence of dental pain in the sample (n = 54,985 in the previous six months was 17.8% (95%CI: 17.5-18.1. Higher prevalence was associated with female gender, age 14 years and over, racial self-identification as black, brown, or indigenous, enrollment in public schools, lower maternal

  16. Lazer sedentário e consumo de alimentos entre adolescentes brasileiros: Pesquisa Nacional de Saúde do Escolar (PeNSE, 2009 Sedentary leisure time and food consumption among Brazilian adolescents: the Brazilian National School-Based Adolescent Health Survey (PeNSE, 2009

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    Lidyane do Valle Camelo

    2012-11-01

    Full Text Available O objetivo deste trabalho foi investigar se o lazer sedentário está associado a um maior consumo regular de alimentos não saudáveis independentemente de indicadores sociodemográficos e de contexto familiar. A análise envolveu 59.809 escolares da Pesquisa Nacional de Saúde do Escolar (PeNSE conduzida em 2009. A variável resposta foi o lazer sedentário, definido como o tempo diário em frente à TV superior a duas horas/dia. As variáveis explicativas de interesse foram o consumo regular de refrigerantes, guloseimas, biscoitos doces e embutidos. Odds ratios (OR e intervalos de 95% de confiança (IC95% foram obtidos por regressão logística múltipla. A prevalência de lazer sedentário foi de 65%. O consumo regular de alimentos não saudáveis foi estatisticamente maior entre os que relataram lazer sedentário, antes e após ajuste por sexo, idade, cor da pele autorreferida, dependência administrativa da escola, índice de bens no domicílio e composição familiar. Esses resultados apontam a necessidade de intervenções que promovam, de forma integrada, lazer e dieta saudável entre os jovens.The objective of this paper was to investigate whether sedentary leisure time was associated with increased regular consumption of unhealthy foods, independently of socio-demographic indicators and family context. The analysis included 59,809 students from the Brazilian National School-Based Adolescent Health Survey (PeNSE in 2009. The response variable was sedentary leisure time, defined as watching more than two hours of TV daily. The target explanatory variables were regular consumption of soft drinks, sweets, cookies, and processed meat. Odds ratios (OR and 95% confidence limits (95%CI were obtained by multiple logistic regression. Prevalence of sedentary leisure time was 65%. Regular consumption of unhealthy foods was statistically higher among students reporting sedentary leisure time, before and after adjusting for sex, age, skin color

  17. Impact of chronic kidney disease on serum tumor markers concentrations

    Institute of Scientific and Technical Information of China (English)

    TONG Hong-li; DONG Zhen-nan; WEN Xin-yu; GAO Jing; WANG Bo; TIAN Ya-ping

    2013-01-01

    Background Serum tumor markers have always been of clinical importance in the diagnosis,monitoring disease progression and therapy efficacy for patients with malignant diseases.However,elevated serum tumor markers are found in some benign conditions,especially in chronic kidney disease (CKD).The elevation of them in CKD might cause confusion and misuse of these tumor markers.We conducted this retrospective study to investigate which of the five widely used tumor markers including carcinoembryonic antigen (CEA),alpha-fetoprotein (AFP),cytokeratin 19 fragment antigen 21-1 (Cyfra21-1),squamous cell carcinoma antigen (SCC) and neuron specific enolase (NSE) are affected markedly by CKD,in order to use them more effectively.Methods Serum tumor marker concentrations,biochemical,hematological parameters,and urinalysis were measured in CKD patients and healthy controls.The positive rate and median tumor markers' level in CKD patients and controls,and those in CKD patients stratified by CKD grade were compared using nonparametric rank tests.Correlation analysis of serum tumor markers and other parameters in CKD patients were performed using the Spearman correlation coefficient.Multivariate Logistic regression analysis was used to estimate the important variables that caused elevated serum concentrations of these markers in CKD patients.Results The overall positive rates and serum concentrations of Cyfra21-1,SCC,CEA in CKD group were significantly higher than those in control group.Positive rate and serum concentrations of those tumor markers increased as kidney function decreased.Both univariate analysis and multivariate regression analysis showed that the elevations of those tumor markers were not only associated with kidney function,but also with nutritional status.Conclusions Serum concentrations of Cyfra21-1,SCC,CEA are significantly influenced by kidney function,as well as nutritional status.Therefore,in clinical work,the indices of kidney function and nutritional

  18. Fascin在非小细胞肺癌中的表达及诊断意义%Expression and diagnosis value of Fascin in non-small cell lung cancer patients

    Institute of Scientific and Technical Information of China (English)

    刘宇罡; 高旭东; 岳文涛

    2013-01-01

    Objective To evaluate the diagnostic significance of Fascin protein in non-small cell lung cancer (NSCLC) patients.Methods Among 110 cases of NSCLC patients and 50 cases of healthy controls,double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was employed to measure the serum levels of Fascin protein,carcinoembryonic antigen (CEA),neuron-specific enolase (NSE),scaleslike squamous cell carcinoma (SCC) antigen,cytokeratin protein 21-1 (CYFRA21-1) and gastrinreleasing peptide (GRP) precursor.Fascin-positive rate (24.4%) in NSCLC patients was significantly higher than that in healthy controls (4%) (P < 0.05).In different clinical stages of non-small cell lung cancer patients,Fascin-positive rate was statistically significant (P < 0.05).The positive rates of Fascin for CEA,CYFRA21-1,NSE,SCC and Pro-GRP were 25.64%,5.1%,5.1%,5.0% and 1.7% respectively.Conclusion As a new tumor marker,Fascin may be used for clinical screening and prognosis in lung cancer.%目的 初步评价Fascin蛋白在非小细胞肺癌(NSCLC)患者中的诊断意义.方法 采用双抗体夹心ELISA法检测内蒙古医学院第一附属医院胸外科及北京胸科医院胸外科2009年10月至2011年5月NSCLC患者共110例和50例健康体检者血清中Fascin蛋白、癌胚抗原(CEA)、神经元特异性烯醇化酶(NSE)、鳞状上皮细胞癌抗原(SCC)、细胞角蛋白21-1(CYFRA21-1)及胃泌素释放肽前体(pro-GRP)的含量.结果 NSCLC患者血清中Fascin阳性率(24.4%)明显高于健康对照组(4.0%)(P<0.05);在不同临床分期的非小细胞肺癌患者中,Fascin阳性率差异有统计学意义(P<0.05);CEA、CYFRA21-1、NSE、SCC、Pro-GRP在非小细胞肺癌的阳性率分别为25.64%、5.1%、5.1%、5.0%、1.7%.结论 Fascin可能作为一种新的肿瘤标志物,在肺癌临床筛查及预后具有一定的临床意义.

  19. NSE和S-100在先天性巨结肠及其同源病诊断中的应用%Application of NSE and S-100 in diagnosing Hirschsprung′s disease and its allied disorders

    Institute of Scientific and Technical Information of China (English)

    姜磊; 刘弋

    2012-01-01

    目的 探讨神经元和神经胶质细胞标志物神经元特异性烯醇化酶(neuron-specific enolase,NSE) 与S-100(soluble-protein 100,S-100) 在先天性巨结肠(Hirschsprung′s disease,HD)及其同源病(Hirschsprung′s disease allied disorders,HAD)诊断中的作用.方法 对45例临床诊断为HD的手术切除标本进行常规HE染色,观察肠神经元及神经节细胞形态和数量的变化,并选择成人结肠肿瘤标本切缘正常组织作对照组进行比较.对HE染色光镜下可疑HD(即HAD)的21例标本进行NSE、S-100蛋白免疫组化测定.结果 对照组结肠壁神经丛中可见染色深浅不一的NSE免疫反应性神经节细胞,神经纤维均匀分布在肠壁各层;神经节细胞胞体对S-100蛋白则表现为细胞状"空白区".HAD组结肠壁分化异常,狭窄段结肠壁神经丛内NSE神经节细胞中NSE呈明显阳性反应,S-100染色可见数量较少的细胞状"空白区".结论 HD的诊断主要根据病理特征而定,在HE染色基础上结合NSE及S-100蛋白免疫组织化学染色能提高确诊率,为HD和HAD鉴别诊断提供依据.%Objective:To explore the values of neuron-specific enolase(NSE) and soluble-protein 100(S-100) of the markers of neuron and neuroglial in the diagnosis of Hirschsprung's disease( HD) and Hirschsprung's disease allied disorders( HAD). Methods: The morphological and quantity change of the neuron and ganglion cells of surgical resection specimens from 45 cases with HD were observed by HE staining, which were compared with normal tissues which located the margin of tumor. The expressions of NSE and S-100 of 21 cases with HAD were analysed by IHC. Results: The heterogeneous NSE immunoreactivities of gangliocytes in knot bowel wall plexus and the evenly distributing of nerve fiber in each layer of the bowel of the control group were observed. The S-100 expression of ganglion cells body showed cell shape " blank area". The differentiation abnormalities bowel, the postive NSE

  20. 良恶性胸腔积液鉴别中SP70检测的临床意义%The significance of protein SP70 detection for differentiating benign and malignant pleural effusion

    Institute of Scientific and Technical Information of China (English)

    杨瑞霞; 曹艳; 王鹏; 许雨乔; 娄鉴芳; 史新惠; 潘世扬; 王芳; 徐建; 黄珮珺; 张燕; 徐娟; 韩月; 朱善军

    2012-01-01

    目的 探讨SP70抗原的检测对鉴别癌性胸腔积液和非癌性胸腔积液的诊断价值.方法 采用病例对照研究.收集2011年7月至2012年2月经临床确诊的肺癌患者胸腔积液108例和非癌性胸腔积液122例,采用双抗体夹心ELISA法对胸腔积液中SP70抗原进行检测;同时与电化学发光法检测CEA、CYFRA21-1和NSE的结果进行比较;采用直接免疫荧光法对胸腔积液脱落细胞上的SP70抗原进行检测,并与HE染色法的脱落细胞学检测结果进行比较,各组间阳性率比较采用x2检验或Fisher确切概率法.结果 癌性胸腔积液患者中SP70、CEA、CYFRA21-1、NSE的阳性率分别为72.2%、58.3%、52.8%和30.6%,显著高于非癌性胸腔积液组(9.8%、13.1%、23.0%和19.7%),SP70、CEA、CYFRA21-1、NSE的特异度分别为90.2%、86.9%、77.0%和80.3%;非小细胞肺癌组的总阳性率显著高于小细胞肺癌组的阳性率(74.3%>0.0%),差异有统计学意义(P=0.02<0.05),癌性胸腔积液患者中SP70的阳性符合率高于传统脱落细胞学HE染色的阳性符合率(72.2%>47.2%),差异有统计学意义(x2=14.03,P<0.05).结论 测定胸腔积液中及脱落细胞上的SP70抗原,能提高癌性胸腔积液诊断的敏感度和特异度.%Objective To investigate the diagnostic value of detection of protein SP70 in differentiating benign and malignant pleural effusion.Methods A case-control study was conducted from July 2011 to February 2012.108 cases of pleural effusion from patients with clinically proven lung cancers and 122 cases of benign pleural effusion were collected.SP70 was detected by Sandwich ELISA,while CEA,CYFRA21-1,NSE were measured by electrochemiluminescence immunoassay for comparison.Meanwhile,protein SP70 on exfoliated cells in pleural effusion was detected by direct immunofluorescence,and was compared with the results of HE staining.The differences between the groups were evaluated by the chisquare

  1. Fatores de risco para acidentes de transporte terrestre entre adolescentes no Brasil: Pesquisa Nacional de Saúde do Escolar (PeNSE Risk factors for road traffic injury among adolescents in Brazil: National Adolescent School-based Health Survey (PeNSE

    Directory of Open Access Journals (Sweden)

    Otaliba Libânio de Morais Neto

    2010-10-01

    Full Text Available Os acidentes de transporte terrestre (ATT causam expressivo número de mortes e hospitalizações entre jovens no mundo, sendo no Brasil responsável por 17,1% dos óbitos entre 10 e 14 anos. Este artigo apresenta resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE, inquérito realizado em uma amostra probabilística de escolares do 9º ano do ensino fundamental de escolas públicas e privadas das capitais brasileiras, em 2009. Foram estimadas as prevalências (e IC95% das situações de risco para acidentes de transporte. Os 60.973 entrevistados indicam que (nos últimos trinta dias: 26,3% (IC95% 25,5%-27,0% referiram nunca ter usado cinto de segurança; 18,5% (IC95% 18,0%-19,1% dos menores de 18 anos referiram ter dirigido veículo motorizado (>1 vez; 18,7% (IC95% 18,1%-19,2% referiram ter sido transportados em veículo conduzido por alguém que consumiu bebida alcoólica (>1 vez e 35% (IC95% 33,8%-36,2% referiram não ter usado capacete pelo menos uma vez quando andava de motocicleta. Os resultados estão de acordo com as elevadas taxas de morbimortalidade de jovens no país por ATT, o que reforça a importância de ações educativas para adolescentes e a necessidade de ações intersetoriais integradas, além de legislação específica e fiscalização rigorosa.Road traffic injuries are the cause of an expressive number of deaths and hospitalizations among young people in the world. In Brazil, it is responsible for 17.1% of all deaths among adolescents aged 10 to 14 years. This article presents the results of the National Adolescent School-based Health Survey (PeNSE, using a probabilistic sample of students in the 9th grade of high schools (public and private of the Brazilian capitals in 2009. The prevalence (and 95%CI of risk factors for road traffic injury among adolescents were estimated. Main results from the 60,973 interviews were: in the last 30 days, 26.3% (25.5% to 27.0% 95%CI reported no use of seat-belts while riding in a

  2. Vivência de violência entre escolares brasileiros: resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE Violence exposures by school children in Brazil: results from the National Adolescent School-based Health Survey (PeNSE

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    Deborah Carvalho Malta

    2010-10-01

    Full Text Available Este artigo apresenta os principais resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE. Aplicou-se questionário em uma amostra de conglomerados de 60.973 estudantes do 9º ano do ensino fundamental de escolas públicas e privadas das capitais dos estados brasileiros e do Distrito Federal, entre março e junho de 2009. Analisam-se prevalências e intervalos de confiança de 95% (IC95% das situações de violência envolvendo adolescentes. Foram identificadas as seguintes situações: insegurança no trajeto casa-escola (6,4%; IC95%: 6,1%-6,8% e na escola (5,5%; IC95%: 5,2%-5,8%; envolvimento em brigas com agressão física (12,9%; IC95%: 12,4%-13,4%, com arma branca (6,1%; IC95%: 5,7%-6,4% ou arma de fogo (4,0%; IC95%: 3,7%-4,3%; agressão física por familiar (9,5%; IC95%: 9,1%-9,9%. As situações de violência foram mais prevalentes entre estudantes do sexo masculino. Houve grande variação segundo as cidades estudadas. Os adolescentes estão expostos a diferentes manifestações de violência nas instituições que supostamente deveriam garantir sua proteção e desenvolvimento saudável e seguro - a escola e o lar. Esses resultados visam apoiar medidas de promoção à saúde e prevenção desses fatores de risco.This article presents the main results of the National Adolescent School-based Health Survey (PeNSE. A questionnaire was applied to a sample of 60,973 students of the 9th year of Junior high school in public and private schools of the Brazilian state capitals and the Federal District, between March and June 2009. The prevalence and confidence interval of 95% (CI 95% of the violence situations involving adolescents were analyzed. The following situations were identified: lack of safety on the way home-school (6.4%; CI95%: 6.1%-6.8% and at school (5.5%; CI95%: 5.2%-5.8%; involving fights with physical aggression (12.9%; CI95%: 12.4%-13.4%, with knife (6.1%; CI95%: 5.7%-6.4% or fire arm (4.0%; CI95%: 3.7%-4.3%; physical

  3. Preoperative serum midkine concentration is a prognostic marker for esophageal squamous cell carcinoma.

    Science.gov (United States)

    Shimada, Hideaki; Nabeya, Yoshihiro; Tagawa, Masatoshi; Okazumi, Shin-ichi; Matsubara, Hisahiro; Kadomatsu, Kenji; Muramatsu, Takashi; Ikematsu, Shinya; Sakuma, Sadatoshi; Ochiai, Takenori

    2003-07-01

    High preoperative serum midkine concentration is associated with poor survival in patients with esophageal cancer, even after radical surgery, and thus may have prognostic value. Midkine (MK), a heparin-binding growth factor, is expressed in numerous cancer tissues, and serum MK (S-MK) concentrations are increased in patients with various neoplasms. The aim of this study is to evaluate the clinical significance of S-MK in patients with esophageal squamous cell cancer (SCC). S-MK was measured by enzyme-linked immunosorbent assay in 135 healthy controls, 16 patients with benign esophageal disease, and 93 patients with primary esophageal SCC before surgery. The serum concentrations of carcinoembryonic antigen (CEA), SCC antigen (SCC-Ag), and cytokeratin 19 fragment (CYFRA21-1) were also evaluated. All patients with esophageal SCC underwent radical esophagectomy. Tumor MK expression was assessed by immunohistochemistry in 14 fresh tumor specimens. To determine whether S-MK is of value as a prognostic factor, the authors conducted a survival analysis using Cox's proportional hazards model. S-MK values in patients with esophageal SCC were significantly higher than those in healthy controls (417 +/- 342 pg/ml vs. 154 +/- 76 pg/ml, P esophageal SCC were classified as positive. MK expression by the tumor was significantly associated with high level of S-MK. High S-MK (>/= 300 pg/ml) was associated with tumor size, immunoreactivity and poor survival. Multivariate analysis indicated that S-MK was an independent prognostic factor. S-MK may be a useful tumor marker for esophageal SCC. Increased preoperative S-MK in patients with esophageal SCC is associated with poor survival.

  4. Effect of systemic vein chemotherapy and internal iliac arterial embolization infusion chemotherapy on angiogenesis and malignant degree of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Gang Chen

    2016-01-01

    Objective:To analyze the effect of systemic vein chemotherapy and internal iliac arterial chemoembolization on angiogenesis and malignant degree of cervical cancer.Methods: A total of 108 cases of patients with middle and advanced cervical cancer were included in the research, and the time range of the research was from February 2014 to December 2015. According to different means of chemotherapy, included patients were divided into observation group 54 cases and control group 54 cases, control group received systemic vein chemotherapy, observation group received internal iliac arterial infusion chemotherapy and embolization treatment, and then differences in the levels of angiogenesis-related indicators, blood flow parameters within tumor, serum illness-related indicators, cervical tumor tissue proliferation-related indicators,etc. were compared between two groups after treatment.Results:Serum VEGFR-2, HIF-1α, vWF and Lam values of observation group after chemotherapy were lower than those of control group; PI, VI, FI, VFI and Vmax values of observation group after treatment were lower than those of control group while RI value was higher than that of control group; serum SCC-Ag, TK1, HE4, CYFRA21-1, IGF-Ⅱ and Gal-9 values of observation group after chemotherapy were lower than those of control group; miR-26b, SCD-1, Cyclin D1 and TLR4 protein expression levels in tumor tissue of observation group after treatment were lower than those of control group while miR-99b protein expression level was higher than that of control group.Conclusions: Internal iliac arterial infusion chemotherapy and embolization can significantly decrease tumor angiogenesis and inhibit tumor cell proliferation, and it is a perfect means of interventional chemotherapy.

  5. Serum cytokeratin 19 fragment 21-1 is a useful tumor marker for the assessment of extramammary Paget's disease.

    Science.gov (United States)

    Kato, Junji; Sumikawa, Yasuyuki; Hida, Tokimasa; Kamiya, Takafumi; Horimoto, Kohei; Kamiya, Shiori; Sato, Sayuri; Takahashi, Hitomi; Sawada, Masahide; Yamashita, Toshiharu

    2017-02-02

    Cytokeratin 19 fragment 21-1 (CYFRA 21-1) has been used as a tumor marker for several malignancies. However, to date, no studies have assessed whether CYFRA 21-1 could be a useful marker for extramammary Paget's disease (EMPD). The present study aimed to evaluate the significance of CYFRA 21-1 as a serum tumor marker for EMPD progression. Concentrations of serum CYFRA 21-1 and carcinoembryonic antigen (CEA) in 13 cases of EMPD were measured prior to undergoing treatment at Sapporo Medical University Hospital from January 2014 to May 2016. Four of the 13 patients had lymph node metastases at diagnosis, but none had distant metastases. Immunohistochemistry indicated that all 13 primary tumors and four metastatic tumors in lymph nodes were positive for cytokeratin 19. Although none of the 13 patients showed high serum CEA levels, six patients (46.2%) had elevated serum CYFRA 21-1. Furthermore, CYFRA 21-1 was reduced in association with post-treatment tumor reduction in all six patients. Among these six patients, four developed recurrence and metastasis during the follow-up period. CYFRA 21-1 was re-elevated in all four of these patients; however, serum CEA was elevated only in the patient with distant metastasis. These results suggest that CYFRA 21-1 is more sensitive compared with CEA, and can be useful as a tumor marker for evaluating tumor progression and treatment efficacy in patients with EMPD.

  6. 肺癌患者血清肿瘤标志物联合检测的临床价值研究%Optimal combination of five tumour markers in prediction of patients with lung cancer

    Institute of Scientific and Technical Information of China (English)

    王洵; 赵弘卿; 吕蕾; 冯金萍; 王昕华; 韩曙光; 鲍亮; 钱晓军; 钱洪献

    2013-01-01

    The study presents data comparing the tumor marker Tumor CA125、CA19-9、CEA、CYFRA21-1 and NSE in the diagnosis of lung cancer. We col ected the blood samples of 116 patients:62 newly diagnosed lung cancer patients, 33 with other pulmonary disease, 21 healthy person. Comparison of the sensitivities and specificity in the detection of lung cancer indicated that CA199+CEA+NSE could be optimal combination for the detection of lung cancer.By selecting appropriate tumour markers in LC patients, we can predict the lung cancer and utilize these markers as complementary tools to establish indications for treatment.%目的:探讨5种肺癌血清肿瘤标志物联合检测的临床价值;方法:应用电化学发光法检测62例肺癌患者、21例健康人和33例肺部良性疾病患者血清中癌抗原125(CA125)、癌抗原19-9(CA19-9)、癌胚抗原(CEA)、细胞角蛋白l9(CYFRA21-1)和神经特异性烯醇化酶(NSE)的含量,计算检测的阳性率及联合检测的敏感性和特异性;结果:肺癌患者的5种血清肿瘤标志物阳性率均高于健康体检组和肺部良性疾病组,差异有统计学意义(P<0.05);在25种联合检测方案中,三联方案CA199+CEA+NSE的特异性与敏感性均较高,与其他组相比具有统计学意义(P<0.O5);结论:合理选择阳性率高的血清肿瘤标志物进行组合,对于提高肺癌筛选的敏感性及特异性具有一定的临床价值。

  7. 脑出血患者血清NSE、GFAP、BDNF水平变化与认知障碍相关性研究%Correlation of serum levels of NSE, GFAP, BDNF and cognitive impairment in patients with intracerebral hemorrhage

    Institute of Scientific and Technical Information of China (English)

    刘宇明; 邓燕华; 许治强; 梁燕玲; 胡佳佳; 林永强

    2016-01-01

    Objective To investigate the serum neuron specific enolase(NSE)and glial fibrillary acidic protein(GFAP), brain derived neural nutrition factor(BDNF)level changes in cerebral hemorrhage patients and the correlation with cognitive impairment. Method 100 cases of acute spontaneous cerebral hemorrhage were selected, and the corresponding treatments were given in the hospital, the seru NSE, GFAP and BDNF were detected. 100 patients were divided into cognitive impairment group(57cases)and non cognitive impairment group(43cases) according to whether there were cognitive impairment. Those three indicators were compared between different levels of cognitive injury(MMSE score)group, and the correlation between the three indicators and MMSE. Results The serum GFAP and NSE were significantly higher in the patients with cognitive impairment than those without cognitive impairment, and BDNF was significantly lower(t=7.039,t=2.247,t=4.847,P<0.01). The serum levels of NSE, GFAP and BDNF were significantly different in the mild injury group, moderate injury group and severe injury group (F=22.752, F=31.506, F=38.294, P<0.01). The levels of serum NSE and GFAP were positively correlated with MMSE scores(r=0.641, r=0.604, P<0.05), and BDNF was negatively correlated with MMSE scores(r=0.582, P<0.05). Conclusion The levels of serum NSE, GFAP and BDNF in patients with cerebral hemorrhage are closely related to cognitive dysfunction after stroke, which can be used to determine the severity of cognitive impairment in patients with cerebral hemorrhage.%目的:探讨脑出血患者血清神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)、脑源性神经营养因子(BDNF)水平变化与认知障碍相关性。方法选择急性自发性脑出血患者100例,入院时均给予相应治疗,并进行 NSE、GFAP、BDNF 检测。将100例患者依据是否发生认知障碍分为认知障碍组(57例)和无认知障碍组(43例)。并

  8. Prevalência de fatores de risco e proteção de doenças crônicas não transmissíveis em adolescentes: resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE, Brasil, 2009 Prevalence of risk health behavior among adolescents: results from the 2009 National Adolescent School-based Health Survey (PeNSE

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2010-10-01

    Full Text Available A adolescência é marcada por transformações e exposição a diversas situações e riscos para a saúde. O estudo atual estima a prevalência dos principais fatores de risco e proteção à saúde dos adolescentes entrevistados em 2009 na Pesquisa Nacional de Saúde do Escolar (PeNSE. A PeNSE, parceria do Instituto Brasileiro de Geografia e Estatística (IBGE com o Ministério da Saúde, usou questionário autoaplicável em palmtop coletando informação de 60.973 estudantes em 1.453 escolas públicas privadas nas 27 capitais. RESULTADOS: consumo de alimentos em cinco dias ou mais na semana: 62,6% comem feijão, apenas 31,5% consomem frutas, 58,3% comem guloseimas e 37% tomam refrigerantes; 43,1% dos alunos são suficientemente ativos fisicamente e 79,5% gastam mais de duas horas diárias em frente à TV. Em relação a tabaco e drogas: 6,3% são fumantes atuais, 71,4% dos alunos disseram já ter experimentado alguma bebida alcoólica. O consumo atual de bebida alcoólica foi de 27% e as drogas ilícitas foram usadas alguma vez na vida em 8,7%. Esses dados geram evidências para orientar a implementação de políticas públicas e realizar ações para minimizar a exposição de fatores de risco dos adolescentes brasileiros.Adolescence is typically a developmental phase characterized by change and experimentation and therefore varying exposure to health hazards. We aimed at estimating the prevalence of protective and risk health behaviors of adolescents interviewed in the 2009 National Adolescent School-based Health Survey (PeNSE. PeNSE, a partnership between the Instituto Brasileiro de Geografia e Estatística (IBGE and the Ministry of Health, using a PDA-based self-administered questionnaire collected information on 60,973 students enrolled at 1,453 public and private schools in the 27 state capitals and the Federal District. RESULTS: among the food items most often consumed ( > or = 5 days per week, were beans (62.6% and fruit (31.5%, but

  9. 肺癌患者血浆Fb、D-D和血清NSE检测的临床意义%Clinical Significance of Measurement of Changes on Plasma Fibrinogen(Fb) D-dimer(D-D) and Serum NSE Levels in Patients with Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    李静; 姚加平; 伏开新; 柏丽丽

    2013-01-01

    Objective To explore the clinical significance of measurement of changes on plasmw Fb, D-D and serum NSE levels in patients with lung cancer. Methods Serum NSE(with RIA) , plasma Fb, D-D(with autoagglutination method)levels were determined in 34 patients with lung cancer and 35 normal health; controls. Results Plasma Fb, D-D and serum NSE levels were significantly higher than those in controls(P < 0. 01), serum NSE level showed positively correlated with Fb, D-D levels(r = 0. 6325, 0.5986, P<0.01). Conclusion Detection of plasma Fb, D-D and serum NSE levels can be useful for prediction of therapeutic efficacy and prognosis in patients with advanced lung cancer and hence provides definite clinical value.%目的:探讨了肺癌患者血浆纤维蛋白原(Fb)、D-二聚体(D-D)和血清NSE水平的变化及临床意义.方法:应用放射免疫分析和自动凝血仪测定法对34例肺癌患者进行了血浆Fb、D-D和血清NSE检测,并与35名正常健康人作比较.结果:肺癌患者血浆Fb、D-D和血清NSE水平均非常显著地高于正常人组(P<0.01),血清NSE水平与Fb、D-D水平呈正相关(r=0.6325、0.5986,P<0.01).结论:检测肺癌患者血浆Fb、D-D和血清NSE水平的变化有助于对疗效评价和预后判断,因而具有一定的临床价值.

  10. AD患者血浆Leptin和血清Hcy、S100B、NSE联合检测的临床意义%Clinical Significance of Determination of Plasma Leptin and Serum Hcy, S100B and NSE Levels in Patients with Alzheimer Disease

    Institute of Scientific and Technical Information of China (English)

    窦焕芝; 卢萌

    2011-01-01

    探讨阿尔茨海默病(AD)患者血浆Leptin和血清Hcy、S100B、NSE水平的变化及临床意义.应用RIA和CLIA法测定32例AD患者血浆Leptin和血清Hcy、S100B、NSE水平,并与30名正常组作比较.结果表明AD患者血浆Leptin和血清Hcy、S100B、NSE水平与正常组比较,均有非常显著地升高(P<0.01),且Leptin水平与Hcy、S100B、NSE水平呈相关(r=0.5982、0.4762、0.6014,P<0.01).检测AD患者血浆Leptin和血清Hcy、S100B、NSE水平对判断病情和评价疗效,均具有一定的临床价值.%To explore the clinical significance of changes of plasma leptin and serun Hcy, Sl00B and NSE levels in patients with Alzheimer Disease (AD). The plasma leptin and serum NSE levels in 32 AD patients and 30 controls were determined by using RIA, and the serum Hcy and Sl00B levels were measured by using CLIA.The results showed that the plasma leptin and serun Hcy, S100B and NSE levels in AD patients were significantly higher than these in controls (P <0.01 ). The plasma leptin levels in AD patients was mutually positively correlated with serum Hcy, Sl00B and NSE levels (r =0. 5982, 0.4762, 0.6014, P <0. 0l ). The detection of plasma leptin and serum Hcy, S100B and NSE levels may be helpful for the prediction of treatment efficiency in patients with Alzheimer disease.

  11. Clinical Significance of Tumor Ang-2 for Lung Cancer Detection%肿瘤标志物--Ang-2对肺癌临床检测意义

    Institute of Scientific and Technical Information of China (English)

    李明琦; 石学魁; 尤琦; 王大为; 贾云鹤; 王锡山; 张鹏宇

    2015-01-01

    Objective: To evaluate the clinical value of angiopoietin-2 in diagnose of lung cancer. Methods: Patients with lung cancer (n=116) and healthy volunteers (n=16) were enrolled. Serum angiopoietin-2 concentrations were measured using en-zyme-linked immunosorbent assay.Serum carcinoembryonic antigen (CEA)、neuron specific enolase;neurone specific enolase (NSE)、cytokeratin fragment 19 (CYFRA21-1) concentrations were measured by electrochemiluminescence(ECL). Serum squamous cell carci-noma antigen (SCC) concentrations were measured by microparticle enzyme immunoassay(MEIA). Results: Patients with lung cancer had higher serum angiopoietin-2 (p<0.05) levels than the control group. With stage progression in nonsmall-cell lung cancer (NSCLC), serum angiopoietin-2 levels increased, and patients with distant metastasis had higher levels than those without metastasis (p<0.005). The combined test of angiopoietin-2 with other tumor markers increased the sensitivity of diagnosis. Conclusions: Our study suggests that serum angiopoietin-2 is a useful clinical marker for detecting lung cancer with distant metastasis and is of potential prognostic value. The combined measurement of CEA、NSE、CYFRA21-1、SCC and angiopoietin-2 was helpful in the diagnosis of lung cancer.%目的:探讨一种新型肿瘤标志物促血管生成素-2(Ang-2)对肺癌诊断、治疗监测、预后判断的应用价值。方法:对112例肺癌患者,包括鳞癌(squamous cell carcinoma ,SC)患者42例、腺癌(adenocarcinoma of lung ,AC)46例、小细胞肺癌(small-cell lung cancer,SCLC)24例,以及16例健康者健康体检者以血清促血管生成素2(Ang-2)检测为主体,同步测定其他相关肿瘤标志物血清癌胚抗原(CEA)、神经元特异性烯醇酶(NSE)、角蛋白19片段(CYFRA21-1)、鳞状细胞癌相关抗原(SCC)。比较病理分型、临床分期及远处转移对血清Ang-2的影响。评价CEA、NSE、CYFRA21-1、SCC与Ang-2

  12. Application of serum SELDI proteomic patterns in diagnosis of lung cancer

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    Zhou Bin

    2005-07-01

    Full Text Available Abstract Background Currently, no satisfactory biomarkers are available to screen for lung cancer. Surface-Enhanced Laser Desorption/ionization Time-of- Flight Mass Spectrometry ProteinChip system (SELDI-TOF-MS is one of the currently used techniques to identify biomarkers for cancers. The aim of this study is to explore the application of serum SELDI proteomic patterns to distinguish lung cancer patients from healthy individuals. Methods A total of 208 serum samples, including 158 lung cancer patients and 50 healthy individuals, were randomly divided into a training set (including 11 sera from patients with stages I/II lung cancer, 63 from patients with stages III/IV lung cancer and 20 from healthy controls and a blinded test set (including 43 sera from patients with stages I/II lung cancer, 41 from patients with stages III/IV lung cancer and 30 from healthy controls. All samples were analyzed by SELDI technology. The spectra were generated on weak cation exchange (WCX2 chips, and protein peaks clustering and classification analyses were made using Ciphergen Biomarker Wizard and Biomarker Pattern software, respectively. We additionally determined Cyfra21-1 and NSE in the 208 serum samples included in this study using an electrochemiluminescent immunoassay. Results Five protein peaks at 11493, 6429, 8245, 5335 and 2538 Da were automatically chosen as a biomarker pattern in the training set. When the SELDI marker pattern was tested with the blinded test set, it yielded a sensitivity of 86.9%, a specificity of 80.0% and a positive predictive value of 92.4%. The sensitivities provided by Cyfra21-1 and NSE used individually or in combination were significantly lower than that of the SELDI marker pattern (P P Conclusion These results suggest that serum SELDI protein profiling can distinguish lung cancer patients, especially NSCLC patients, from normal subjects with relatively high sensitivity and specificity, and the SELDI-TOF-MS is a potential tool

  13. 脑卒中患者血清 Hcy、NSE、PAF 水平变化及临床意义%The relationship of the levels of Hcy, NSE, PAF and nerve funciton deficit in stroke patients

    Institute of Scientific and Technical Information of China (English)

    邱毅; 吴波; 白昌睿; 屈建良

    2016-01-01

    目的:观察脑卒中患者血清同型半胱氨酸( Hcy)、神经元特异性烯醇化酶( NSE)、血小板活化因子( PAF)水平变化及临床意义。方法选取2013年6月—2015年6月陕西省汉中职业技术学院附属医院内二科收治脑卒中患者120例为观察组,根据美国国立卫生研究院卒中量表( NHISS)评分分为轻度卒中亚组45例,中度卒中亚组42例,重度卒中亚组33例。另选取同期健康体检者100例为对健康照组,采用ELISA法测定2组血清Hcy、NSE、PAF水平,并分析其与NHISS评分、Barthel指数( BI)、格拉斯哥昏迷量表评分( GOS)的关系。结果观察组患者血清Hcy、NSE、PAF水平显著高于健康对照组( t =12.986、14.278、10.425, P <0.05);且重度卒中亚组患者高于轻中度卒中亚组患者( F =8.363、9.123、10.562, P <0.05);脑卒中存活亚组患者显著低于病死亚组( t =11.464、9.758、8.923, P <0.05)。脑卒中患者入院后第3天NHISS评分低于入院时( P <0.05),而BI评分、GOS 评分高于入院时( P <0.05)。经Pearson单因素分析显示,脑卒中患者血清Hcy与NHISS评分、BI评分、GOS评分呈正相关( r =0.345、0.369、0.337, P <0.05),NSE、PAF与NHISS评分呈正相关( r =0.372、0.342, P <0.05),与BI评分、GOS评分无相关性( r =0.122、0.108、0.013、0.098, P >0.05)。结论脑卒中患者血清Hcy、NSE、PAF水平与神经功能缺损程度有密切的关系,可作为其病情进展及预后的评价指标。%Objective To observe the levels of serum homocysteine ( Hcy ) ,neuron specific enolase ( NSE ) and platelet activating factor ( PAF) in stroke patients and its clinical significance .Methods Selected from June 2013 to 2015 June, Shanxi Province Hanzhong vocational and technical college affiliated hospital admitted 120 casesof

  14. Utility of NSE, ProGRP and LDH in Diagnosis and Treatment in Patients with Small Cell Lung Cancer%血清NSE、ProGRP和LDH在小细胞肺癌诊断治疗中的作用

    Institute of Scientific and Technical Information of China (English)

    彭彦; 王燕; 李峻岭; 郝学志; 胡兴胜

    2016-01-01

    Background and objective Small cell lung cancer (SCLC) is a rapidly growing tumor with character-istic of neuroendocrine cellular function. Neuron speciifc enolase (NSE), pro-gastrin-releasing peptide (ProGRP) and lactic dehydrogenase (LDH) are valuable in diagnosis and treatment of SCLC. By analyzing the variation of NSE, ProGRP and LDH before and atfer treatment, the aim of this study is to investigate the effcacy of tumor markers in diagnostic staging, therapeu-tic evaluation and prediction of disease relapsing.Methods Patients with SCLC who receiving the ifrst line chemotherapy in Cancer Hospital, Chinese Academy of Medical Sciences were enrolled and retrospectively analyzed. Clinical characteristic (includes NSE, ProGRP and LDH level before and atfer 2 cycles chemotherapy), effcacy evaluation, progression-free survival (PFS) were analyzed.Results Before treatment, Serum NSE, ProGRP and LDH in patients with extensive disease (ED) were signiifcantly higher than those with limited disease (LD)(allP4 cycles chemotherapy and with obvious decrease of ProGRP than those who accepted ≤4 cycles chemotherapy and with less obvious decrease of ProGRP in LD group; ED patients with no more than 2 distant metastasis, normal LDH level before treat-ment and obvious decrease of ProGRP atfer chemotherapy had lower short term relapse risk. In addition, the types of relapse (sensitive relapse, drug resistance relapse and refractory relapse) were negatively correlated with decrease of ProGRP (P=0.044). By multivariate analysis, numbers of chemotherapy cycle was independent prognostic factor for PFS in LD SCLC; numbers of distant metastasis and decrease of ProGRP were independent prognostic factors for PFS in ED SCLC.Conclusion Increase level of serum tumor markers is related to tumor burden. Decrease level of ProGRP atfer treatment may prognose effcacy and relapse risk.%背景与目的小细胞肺癌(small cell lung cancer, SCLC)是一种生长迅速、具有神经内分泌

  15. Clinical Significance of Determination of Serum and Cerebro-Spinal (CSF) Fluid Levels of Neuron Specific Enolase (NSE) in Patients with Brain Injury%脑外伤患者血清和脑脊液NSE测定的临床意义

    Institute of Scientific and Technical Information of China (English)

    周彦

    2004-01-01

    目的:探讨了颅脑外伤患者血清和脑脊液神经元特异性烯醇化酶(NSE)的水平及其临床意义.方法:采用放射免疫分析对36例颅脑外伤患者进行了血清和脑脊液NSE测定.结果:颅脑外伤患者血清和脑脊液NSE水平均显著地升高,尤以重型组为甚.结论:测定颅脑外伤患者血清和脑脊液NSE水平对了解病情,并有助于判断愈后.

  16. 联合检测七种血清肿瘤标志物在肺癌诊断中的价值%Diagnostic value of joint detection of seven kinds of serum tumor markers in lung cancer

    Institute of Scientific and Technical Information of China (English)

    马芳芳; 王厚照; 刘青

    2013-01-01

    目的 探讨血清癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原199(CA199)、鳞状细胞癌抗原(SCC-Ag)、神经元特异性烯醇化酶(NSE)、细胞角蛋白19片段(CYFRA211)、胃泌素释放肽前体(ProGRP)对肺癌的诊断价值.方法 采用化学发光法检测60例肺癌患者和52例健康者血清中CEA、CA125、CA199、SCC Ag、NSE、CYFRA211和ProGRP水平和阳性率.结果 肺癌组血清CEA、CA125、CA199、SCC-Ag、NSE、CYFRA211和ProGRP水平和阳性率与健康对照组相比,差异均有统计学意义(P<0.05);联合检测7种肿瘤标志物诊断肺癌的灵敏度明显高于单项检测.结论 上述7种血清肿瘤标志物对肺癌的辅助诊断有一定价值,其联合检测对肺癌的早期诊断具有重要意义.%Objective To investigate the diagnostic value of carcinoembryonic antigen (CEA),carbohydrate antigen 125 (CA125),carbohydrate antigen 199(CA199),squamous cell carcinoma antigen(SCC-Ag),neuron-specific enolase(NSE),cytokeratin 19 fragment(CYFRA211),gastrin releasing peptide precursor(ProGRP) in lung cancer.Methods The serum content and the positive rate of CEA,CA125,CA199,SCC-Ag,NSE,CYFRA211,ProGRP of serum tumor markers were measured by chemiluminoimmunoassay in 60 patients with lung carcinoma and 52 healthy suhjectseople.Results The levels and positive rates of CEA,CA125,CA199,SCC-Ag,NSE,CYFRA211,ProGRP of serum tumor markers in lung cancer group were higher than those of healthy controls(P<0.05),and combined detection of seven kinds of tumor makers had high sensitivity for lung cancer diagnosis.Conclusion The seven kinds of serum tumor markers are helpful for diagnosis of lung cancer.Joint detection is very significant for the early diagnosis of lung cancer.

  17. 慢性阻塞性肺疾病、肺炎和肺癌患者血清HMGB1水平及临床意义%The elevated level of HMGB1 in serum and its clinical role in patients with COPD, pneumonia and lung cancer

    Institute of Scientific and Technical Information of China (English)

    崔正森; 李菡; 姜宝珍; 张志红

    2014-01-01

    Objective To explore the clinical significance of high mobility group box 1 ( HMGB1 ) level in acute exacerbation chronic obstructive pulmonary disease( AECOPD) , pneumonia and lung cancer patients. Methods Based on the criteria, 40 AECOPD patients, 40 pneumonia patients, and 30 non-small cell lung cancer patients were recruited in this clinical study, meanwhile 30 healthy volunteers were recruited as controls. The peripheral blood samples were collected before any treatment was performed on them and the concentrations of HMGB1 were detected by ELISA. Specific bio-markers of lung cancer such as carcinoma embryonic antigen( CEA) , neuron-spe-cific enolase(NSE) and cytokeratin 19 fragment(CYFRA21-1) were measured in cancer patients and healthy vol-unteers. Other clinical data such as white blood cell( WBC) count and C reactive protein( CRP) were also collect-ed and analyzed. Results The serum concentrations of HMGB1 in AECOPD, pneumonia and lung cancer patients were higher than those in the control group respectively(P<0. 01). In addition, HMGB1 had a positive correlation-ship with the leukocyte count ( P=0. 008 , P =0. 002 ) and CRP ( P =0. 001 , P =0. 001 ) level in AECOPD and pneumonia patients; in lung cancer group, HMGB1 was correlated positively with the tumor bio-markers ( P =0. 036,P=0. 008). Conclusion The serum level of HMGB1 is significantly high in patients of AECOPD, pneu-monia and lung cancer,which indicates that HMGB1 may play a very important role in the development and severity of inflammation and tumor in respiratory system.%目的:探讨血清高迁移率族蛋白B1( HMGB1)在慢性阻塞性肺疾病急性加重期( AECOPD)、肺炎、肺癌患者中的表达水平及意义。方法按照入组标准纳入AECOPD患者40例,肺炎患者40例,非小细胞肺癌( NSCLC )患者30例,健康对照者30例;抽取AECOPD、肺炎、肺癌患者及健康对照者全血,检测 HMGB1( ELISA法)水平,外周血白细胞(WBC)计数、C-反应蛋白(CRP)水

  18. Cytokeratin 19 fragment antigen 21-1 as an independent predictor for definitive chemoradiotherapy sensitivity in esophageal squamous cell carcinoma

    Institute of Scientific and Technical Information of China (English)

    YAN Hong-jiang; WANG Ren-ben; ZHU Kun-li; JIANG Shu-mei; ZHAO Wei; XU Xiao-qing; FENG Rui

    2012-01-01

    Background Patients with esophageal squamous cell carcinoma (ESCC) undergoing definitive chemoradiotherapy (CRT) seem to have a disparity in therapeutic response.The identification of CRT sensitivity-related clinicopathological factors would be helpful for selecting patients most likely to benefit from CRT.Cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) and carcinoembryonic antigen (CEA) have been reported as useful tumor markers for esophageal cancer.The aim of this study was to examine the predictive value of CYFRA21-1 in comparison with CEA and other clinicopathological factors in patients with ESCC treated with definitive CRT.Methods Pretreatment serum CYFRA21-1 and CEA levels were measured by immunoradiometric assays.The relationships between pretreatment clinicopathological factors and the efficacy of CRT were analyzed.Overall survival (OS) was estimated by univariate and multivariate analysis.Results The results from a univariate analysis indicated that the efficacy of CRT was significantly associated with the serum levels of CYFRA21-1 and CEA before treatment (P=0.001 and P=0.023,respectively).It also indicated that the efficacy of CRT was significantly associated with the pretreatment tumor location (P=0.041).By Logistic regression analysis,the independent predictive factor associated with efficacy of CRT was CYFRA21-1 (P=0.002).The OS of the patients with high CYFRA 21-1 levels was worse than that of those with low CYFRA21-1 levels (P=0.001).In multivariate analysis,a low level of CYFRA21-1 was the most significant independent predictor of good OS (P=0.007).Conclusions CEA and tumor location may be useful in predicting the sensitivity of ESCC to CRT.CYFRA21-1 may be an independent predictor for definitive CRT sensitivity in ESCC.

  19. ACI患者治疗前后血浆ET-1和血清NSE、NPY联检的临床意义%Clinical Significance of Changes of Plasma ET-1 and Serum NSE,NPY Levels Both Before and After Treatment in Patients with Acute Cerebral Infarction

    Institute of Scientific and Technical Information of China (English)

    吴健丰

    2011-01-01

    目的:评估了急性脑梗死(ACI)患者治疗前后血浆内皮素-1(ET-1)和血清神经元特异性烯醇化酶(NSE)、神经肽-Y(NPY)水平的变化及临床意义.方法:应用放射免疫分析对32例ACI患者进行了治疗前后血浆ET-1和血清NSE、NPY检测,并与35名正常健康人作比较.结果:在治疗前血浆ET-1和血清NSE、NPY水平均非常显著地高于正常人组(P<0.01),经治疗3个月后与正常人组比较仍有显著性差异(P<0.05).结论:测定ACI患者治疗前后血浆ET-1和血清NSE、NPY水平对观察病情和预后判定具有重要的临床价值.%Objective To explore the changes of plasma ET-1 and serum NSE, NPY levels both before and after treatment in patients with acute cerebral infarction and their significance. Methods Plasma ET-1 and serum NSE, NPY levels were measured with RIA in 32 patients with acute cerebral infarction both before and after treatment as well as in 35 controls. Results Before treatment plasma ET-1 and serum NSE, NPY levels were significantly higher than those in contrrols ( P < 0.01 ). After for 3 months of treatment,the levels dropped markedly, but still remained significant higher ( P < 0.05 ). Conclusion Changes of plasma ET-1 and serum NSE, NPY contents both before and after treatment might be of prognostic importance in patients with acute cerebral infarction.

  20. 急性颅脑损伤患者血清甲状腺、PRL和NSE水平的变化及临床意义%ClinicaL Significance of measurement of changes of Serum Thyoids,PRL and NSE levels in patients with acute craniocerebral in jury

    Institute of Scientific and Technical Information of China (English)

    潘晴

    2013-01-01

      目的探讨了急性颅脑损伤患者血清甲状腺素、PRL和NSE水平的变化及临床意义。方法应用化学放光法和放免法对33例急性颅脑损伤患者进行了血清甲状腺素、PRL和NSE水平检测,并与35名正常健康人作比较。结果急性颅脑损伤的血清TSH、PRL、NSE水平非常显著地高于正常人组(P<0.01),而TT3水平非常显著地低于正常人组(P<0.01),血清TT3水平与TSH、PRL、NSE水平呈显著负相关(r=--0.4012、-0.5718、-0.5922、P<0.01)。结论检测急性颅脑损伤患者血清甲状腺素、PRL、和NSE水平的变化对诊断治疗和估计病情轻重有一定的临床价值。%objective:To explore the clinicnl signi-ficance of changes of Serum Thyoids, PRL and NSE levels in patients With acute craniocerebral injury.Methods:serum Thyoids. PRL(withCLIA) serum NSE(withRIA) levels mere? determined in 33 patients with acute craniocevebral injury and 35 incontrols. pesult: serumTSH、PRL、NSE levels were signiticantly higher than , those in controls(P<0.01), but serum TT3 Levels were significmtliy Lower than those in controls(P<0.01), Seran TT3 levels were negative correlated with TSH、PRL、NSE(r=-0.4012、-0.5718、-0.5922、P<0.01).Conclusions: Detection of serum Thyoid、PRL and NSE Levels might he of helpful assessment of che severing of d:sease and prognosis, much of clinical Values.

  1. 病毒性脑炎患儿S100B蛋白、NSE、sVCAM-1和神经肽Y水平变化的临床意义%Clinical significance of the level change of S100B protein, NSE, sVCAM-1 and neuropeptide Y in children with viral encephalitis

    Institute of Scientific and Technical Information of China (English)

    张永英

    2012-01-01

    Objective To explore the clinical significance of the level change of serum S100B protein, neuron enolase (NSE), soluble vascular endothelial cell adhesion molecules (sVCAM-1) and neuropeptide Y (NPY) in children with viral encephalitis. Methods 32 cases of children viral encephalitis with central nervous system infection were selected as the viral encephalitis group, and according to the illness was divided into light disease group and severe disease group, at the same time, 32 cases without central nervous system infection (by lumbar puncture) of children were chose as control group. S100B protein, NSE, sVCAM-1 and NPY level of the serum and cerebrospinal fluid in children were detected, and the results were compared. Results The levels of S100B protein, NSE, sVCAM-1 and neuropeptide Y (NPY) in the viral encephalitis group were obviously higher than those in the control group, there were significant differences (P < 0.05). For the viral encephalitis children, S100B protein, NSE, sVCAM-1 and neuropeptide Y (NPY) level in serum and cerebrospinal fluid were significantly lower in light disease group than those in severe group, there were significant differences (P < 0.05). Conclusion There is a correlation between the change and illness weight of viral encephalitis children cerebrospinal fluid and serum S100B protein, NSE, sVCAM-1 and neuropeptide Y level, which has an important value to judge the illness.%目的 探讨病毒性脑炎患儿脑脊液及血清中S100B蛋白、神经元烯醇化酶(NSE)、可溶性血管内皮细胞黏附分子(sVCAM-1)和神经肽Y(NPY)水平变化的临床意义.方法 选取32例有中枢神经系统感染的病毒性脑炎患儿为病毒性脑炎组,并根据病情分为轻症组和重症组,选择同期住院的经腰椎穿刺确定无中枢神经系统感染的病毒性脑炎患儿32例作为对照组.检测各组患儿血清和脑脊液中S100B蛋白、NSE、sVCAM-1和NPY的水平,并将结果进行比较.结果 病毒性脑炎

  2. 血清肿瘤标志物 NSE、CA-125在非霍奇金淋巴瘤诊断及预后判定中的临床价值%Clinical significance of serum tumor makers NSE,CA125 in the diagnosis and prognosis assessment of non-Hodgkin’ s lymphoma

    Institute of Scientific and Technical Information of China (English)

    房丽; 王轶楠; 马守东; 肖建波; 李海丽; 王玲玲; 刘翠华

    2014-01-01

    目的:探讨血清肿瘤标志物NSE和CA-125在非霍奇金淋巴瘤( NHL)中的表达情况及临床意义。方法采用电化学发光免疫测定法测定56例NHL患者血清肿瘤标志物NSE和CA-125的表达水平,判定NSE和CA-125的表达与NHL患者临床特征及其预后的相关性。结果56例NHL患者化疗前血清NSE值(34 U.63±8.54)μg/L,CA-125(47.59±9.53)μg/L,均明显高于对照组,差异有统计学意义( P <0.05);且NSE、CA-125表达水平的高低与NHL患者B症状、临床分期、IPI预后指数及治疗效果等临床特征相关( P <0.05)。结论血清肿瘤标志物NSE、CA-125联合定量检测可提高NHL诊断敏感性,同时对NHL临床分期、疗效判定及预后等方面具有一定临床意义。%Objective To investigate the clinical significance of the two serum tumor markers-neuron specific enolase ( NSE) and carbohydrate antigen 125 ( CA-125 ) in the diagnosis and prognosis evaluation of non-Hodgkin ’ s lymphoma ( NHL) .Methods The serum levels of NSE and CA-125 in 56 patients with NHL were measured by electroche miluminescence immunoassay .The correlation between the expressions of NSE , CA125 and clinical characteristics as well as prognosis of patients with NHL were analyzed.Results Before chemotherapy the serum levels of NSE [( 34.63 ± 8.54)μg/L] and CA-125 [(47.59 ±9.53)μg/L] in 56 patients with NHL were significantly higher than those in control group ( P <0.05),furthermore,the expression levels of NSE and CA-125 were correlated with patient ’s symptoms,clinical stage,IPI prognosis index and therapeutic effect.Conclusion The quantitive detection of NSE combined with CA 125 plays an important role in diagnosis,clinical staging and prognosis assessment for patients with NHL ,which can increase diagnostic sensitivity.

  3. 新生儿缺氧缺血性脑病颅脑MRI-Apgar评分、血清NSE相关性研究%Study on brain MRI and its relations to Apgar scores, serum level of NSE in patients with neonatal hypoxic ischemic encephalopathy

    Institute of Scientific and Technical Information of China (English)

    丁燕霞; 李晓春

    2011-01-01

    Objective To invastigate the change of cerebral MRI of neonatal Hypoxic Is-chemic Encephalopathy(HIE), and the relation between the serum neuron - specific enolase(NSE) and Apgar scores. Methods One hundred HIE cases were chosen as experimental group, including 50 preterm cases and 50 term neonates. Based the degree of MRI, the subjects in the experimental group were classified into HIE mild and HIE moderate and severe. Another forty normal ones were chosen as control group. Blood samples were collected in all the research objects for NSE detecting. MRI was performed 3~7 days after birth in the experimental group. Results In full term neonates and preterm neonates, as the aggravation of the degrees of MRI, the serum NSE levels got increased and Apgar scores got decreased. Compared with the control group and the HIE mild group, the NSE levels in the HIE moderate and severe group had significant differences. However, the differences between the mild HIE group and the control group was insignificant. Differences of 5 min Apgar score between any two groups were significant. Conclusion Serum NSE level and Apgar score can be used as the early objective markers for evaluating the prognosis of brain damage in hypoxic is-chemic encephalopathy, and they are concordance with the cerebral MRI degrees. We can perform early diagnosis and evaluation of HIE by utilizing the two indexes.%目的 探讨新生儿缺氧缺血性脑病(HIE)颅脑磁共振成像(MRI)改变与血清神经元特异性烯醇化酶(NSE)及新生儿评分(Apgar评分)之间的关系.方法 选取HIE患儿100例(早产儿50例,足月儿50例)为实验组,依据颅脑MRI表现程度分为轻度HIE和中重度HIE组;另选同期非HIE新生儿40例为对照组,均在其生后行Apgar评分、检测血清NSE浓度,并于生后3~7 d行颅脑MRI检查.结果 HIE足月儿和早产儿随着MRI表现程度加重,血清NSE值逐渐增高,Apgar评分逐渐降低.中重度组与轻度组、对照组相比NSE水平

  4. The Clinical Clues of Pulmonary Alveolar Proteinosis: A Report of 11 Cases and Literature Review

    Directory of Open Access Journals (Sweden)

    Qiongya Mo

    2016-01-01

    Full Text Available Pulmonary alveolar proteinosis (PAP is a rare interstitial lung disease characterized by the abnormal alveolar accumulation of surfactant components. The diagnosis of PAP can be easily missed since it is rare and lacks specific clinical symptoms. It is of great importance to have a better understanding of the crucial clue to clinically diagnose PAP and take PAP into consideration in the differential diagnosis of interstitial pulmonary diseases or other diseases with similar manifestations. Here, we analyze the clinical characteristics of 11 cases of PAP patients in local hospital and review the relevant literature in order to provide more information in diagnosis and management of PAP. In our observation, cyfra21-1 and neuron-specific enolase (NSE known as tumor markers probably can be useful serum markers for diagnosis of PAP. As for the method of pathologic diagnosis, open-lung biopsy was the gold standard but now it is less required because findings on examination of bronchoalveolar lavage fluid (BALF can help to make the diagnosis. We also have deep experience about when and how to carry out lung lavage.

  5. A ssessment of the malignant biology and bone metabolism after transcatheter vertebral arterial chemoembolization combined with zoledronic acid treatment of vertebral metastasis from lung cancer

    Institute of Scientific and Technical Information of China (English)

    Wei Lu; Zhe Zhang; Chao Yang; Jia-Cheng Zhang; Jun-Peng Ma; Peng Du; Jian Yang; Fu-Qiang Jiang; Xin-Li Jin; Peng Xie

    2016-01-01

    Objective:To study the malignant biology and bone metabolism after transcatheter vertebral arterial chemoembolization combined with zoledronic acid treatment of vertebral metastasis from lung cancer.Methods: A total of 46 cases of patients with vertebral metastasis from lung cancer treated in our hospital between May 2012 and December 2015 were selected and divided into two groups, observation group (n=23) received transcatheter vertebral arterial chemoembolization combined with zoledronic acid treatment and control group only received transcatheter vertebral arterial chemoembolization therapy. Eight weeks after treatment, serum was collected to determine the levels of tumor markers, invasive molecules and bone transformation indicators.Results:8 weeks after treatment, serum CEA, NSE, Cyfra21-1, TK1, RCAS1, CatB, CatD, CatX, MMP7, MMP9, MMP13, N-MID, BALP, OC, PINP,β-CTX, NTX, ICTP and TRACP5b levels of observation group were significantly lower than those of control group.Conclusions:Transcatheter vertebral arterial chemoembolization combined with zoledronic acid treatment can more effectively kill the cancer cells in vertebral metastases, inhibit the secretion of protease and regulate bone metabolism balance.

  6. Effect of S-1 + cisplatin and gemcitabine + cisplatin on malignant indexes in serum and tumor tissue of advanced NSCLC patients with wild-type EGFR

    Institute of Scientific and Technical Information of China (English)

    Can Chen; Qing Fang; Qing-Yuan Zhou

    2016-01-01

    Objective:To study the effect of S-1 + cisplatin and gemcitabine + cisplatin on malignant indexes in serum and tumor tissue of advanced NSCLC patients with wild-type EGFR. Methods: The medical records of 58 advanced non-small cell lung cancer patients with wild-type EGFR were retrospectively analyzed, patients who received S-1 + cisplatin chemotherapy were included in the experimental group, patients who received gemcitabine + cisplatin chemotherapy were included in the control group, and then the levels of tumor markers in serum and the expression levels of proto-oncogenes and tumor suppressor genes in tumor tissue were determined.Results:After treatment, serum tumor markers CEA, NSE, Cyfra21-1, PCDGF, VEGF and TK1 levels of experimental group were significantly lower than those of control group; Pim-1, LUNX, PFKFB3, PFKFB4 and Ki67 levels in tumor tissue of experimental group were significantly lower than those of control group, and TCF21, PDCD5, ASPP1, ASPP2, p53 and EFEMP1 levels were significantly higher than those of control group. Conclusions: S-1 + cisplatin has better curative effect on advanced NSCLC with wild-type EGFR than gemcitabine + cisplatin, and can more effectively kill cancer cells and regulate the expression of proto-oncogenes and tumor suppressor genes.

  7. Effect of zoledronic acid injection combined with radiopharmaceutical 89SrCI2 therapy on the growth and clinical symptoms of lung cancer bone metastasis

    Institute of Scientific and Technical Information of China (English)

    Jia-Lun Zhu; Zhi-Yong Deng; Chuan-Zhou Yang

    2016-01-01

    Objective:To find the effect of zoledronic acid injection combined with radiopharmaceutical 89SrCI2 therapy on the growth and clinical symptoms of lung cancer bone metastasis.Methods: A total of 102 lung cancer patients with bone metastases were included in the study and randomly divided into observation group and control group (n=51) according to different treatment they received. Control group received zoledronic acid injection therapy alone, observation group received zoledronic acid injection combined with radiopharmaceutical 89SrCI2 therapy, and then differences in the growth of lung cancer bone metastasis, bone metabolism, tumor markers and alkaline phosphatase, pain score and pain-related mediator levels,etc. were compared between two groups.Results: Number of metastases of observation group after treatment was less than that of control group, and serum bone metabolism indexes OPG, BSP, TRACP-5b, ICTP and BAP levels, serum tumor markers CYFRA21-1, CEA, NSE, CA125 and BALP levels as well as serum pain-related mediators PGE2, ET-1 and TNF-α levels were lower than those of control group (P<0.05).Conclusions:Zoledronic acid injection combined with radiopharmaceutical89SrCI2 therapy can contain the growth of lung cancer bone metastasis, optimize bone metabolism state while alleviate patients’ perception of pain.

  8. Clinical value of tumor markers SCCA,TPS,NSE and CYFERA21-1 detection in diagnosis of lung cancer%肿瘤标志物SCCA 、TPS、NSE和CYFERA21-1检测在肺癌中的临床价值

    Institute of Scientific and Technical Information of China (English)

    潘秋荣; 徐勤燕

    2015-01-01

    Objective To explore the clinical value of tumor marker squamous cell carcinoma antigen(SCCA) ,tissue polypeptide specific antigen(TPS) ,neuron‐specific enolase(NSE) ,cytokeratin fragment 19(CYFRA21‐1) detections in the patient with lung cancer .Methods 70 patient with newly diagnosed lung cancer(including 26 cases of squamous cell carcinoma ,23 cases of adenocar‐cinoma and 21 cases of small cell carcinoma) ,40 cases of lung benign diseases and contemporaneous 30 individuals undergoing the physical examination were randomly selected as the lung cancer group ,lung benign disease group and healthy control group respec‐tively .Serum tumor markers of SCCA ,TPS ,NSE and CYFERA21‐1 were detected in all cases .Then the detection results were per‐formed the statistical analysis .Results The levels of SCCA ,TPS ,NSE and CYFERA21‐1 in the lung cancer group were significant‐ly higher than those in the lung benign diseases group and healthy control group ,the differences were statistically significant (P0 .05) .The combination detection of SCCA , TPS ,NSE and CYFERA21‐1 greatly increased the sensitivity and accuracy for diagnosing lung cancer .Conclusion The combina‐tion detection of SCCA ,TPS ,NSE and CYFERA21‐1 has very high clinical value for the diagnosis ,pathological typing and assisted clinical therapy of lung cancer .%目的:探讨肿瘤标志物鳞状上皮细胞癌抗原(SCCA)、组织多肽特异性抗原(TPS)、神经原特异性烯醇化酶(NSE)和细胞角蛋白‐19片断抗原(CYFERA21‐1)检测在肺癌患者中的临床价值。方法随机选择2012年2月至2014年10月来该院初诊确诊肺癌患者70例(其中鳞癌26例、腺癌23例、小细胞癌21例),肺良性疾病患者40例(其中肺炎19例、支气管炎16例、肺结核5例),同期健康体检者30例进行SCCA、TPS、NSE和CYFERA21‐1的检测。然后进行统计学分析。结果肺癌组血清肿瘤标志物SCCA、TPS、NSE和CYFERA21

  9. 二十五味珊瑚丸对D-半乳糖衰老大鼠神经元特异性烯醇化酶表达的影响%Effect of 25 Odors Coral Pills on Pyramidal Cell and the Expression of NSE in Hippocampus of D-galactose Induced Brain Aging Rats

    Institute of Scientific and Technical Information of China (English)

    杨春; 李鹏; 罗远带; 杜以宽; 黄福开; 甄丽芳; 胡贤达; 杨勤劳; 原林

    2015-01-01

    探讨二十五味珊瑚丸(25 Odors Coral Pills)对D-半乳糖(D-gal)衰老大鼠海马锥体细胞形态和神经元特异性烯醇化酶(NSE)表达的影响.采用颈背部皮下注射D-gal模拟衰老大鼠模型.治疗组于造模第6周,给予25 Odors Coral Pills灌胃2周,之后取各组脑组织行相关检测.与模型组比较,25 Odors Coral Pills组大鼠海马CA1、CA3区锥体细胞层细胞丢失较少,细胞排列较紧密、整齐;DG区NSE阳性的细胞减少,胞体较小,突起较细;海马NSE表达下降,有统计学差异(P<0.05).D-gal衰老模型鼠海马锥体细胞衰老变性且NSE的表达增多;25 Odors Coral Pills可抑制D-gal衰老鼠海马锥体细胞的衰老变性和NSE的表达.

  10. PET - CT联合检测肿瘤标志物对肺癌术后随访的价值%The clinical value of PET - CT combined with detection of serum tumor markers in follow-up of patients with lung cancer postoperatively

    Institute of Scientific and Technical Information of China (English)

    江吕泉; 高坤祥; 郑建; 陈建; 吴昊

    2011-01-01

    目的 探讨肿瘤标志物和正电子发射断层/计算机断层扫描( PET - CT)对肺癌术后随访的作用及相互关系.方法 97例肺癌术后患者做全身PET - CT检查,并在l周内检测血癌胚抗原(carcinoembryonic antigen,CEA)、细胞角蛋白19片段(cytokeratin 19 fragment 21 -1,CYFRA 21 -1)和神经元特异性烯醇化酶(neuron specific enolase,NSE),同时与最终诊断进行比较,分析两种检查之间差异和相互关系.结果 97例中,89例诊断肺癌复发或转移.肿瘤标志物诊断肺癌术后复发或转移的敏感性为68.5%,特异性75.0%,准确性为69.1%;PET - CT诊断肺癌术后复发或转移的敏感性为92.1%,特异性75.0%,准确性为90.7%.两者联合诊断的敏感性为100%,特异性为92.0%,准确性为95.6%.结论 PET - CT诊断肺癌术后的复发或转移敏感性和准确性较肿瘤标志物检测高,肿瘤标志物对PET - CT诊断具有补充作用,两者联合应用对于肺癌术后的随访有重要的临床价值.%Objective To study the value of and correlations between positron emission tomography/computed tomography ( PET/CT) and serum tumor markers in the follow - up of lung cancer after operation. Methods In 97 patients with a surgical history of lung cancer, PET/CT was performed and serum carcinoembryonic antigen ( CEA), cytokeratin 19 fragment 21 - 1 (CYFRA21 - 1) and neuron specific enolase (NSE) were detected within the following week. The results were compared with the final diagnosis. The difference and correlations between the two methods of examination was analyzed. Results Recurrence or metastasis was found in 89 of the 97 patients with a surgical history of lung cancer. The sensitivity , specificity and accuracy of tumor marker detection for diagnosis of recurrence or metastasis were 68.5% ,75.0% and 69. 1% respectively, those of PET/CT were 92. 1% ,75.0% and 90.7% respectively, and those of combined PET/CT with detection of tumor markers were 100% ,92. 0

  11. Diagnostic value of combination of 99mTc-MIBI imaging,multi-slice spiral CT and tumor marker measurements for lung cancer%99mTc-MIBI肺显像、多排螺旋CT和肿瘤标志物检测对肺癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    党国际

    2009-01-01

    目的 探讨99Tc-甲氧基异丁基乙腈(99Tc-MIBI)亲肿瘤显像、多排螺旋CT、血清肿瘤标志物[癌胚抗原(CEA)、细胞角蛋白19片断(CYFRA21-1)、神经元特异性烯醇化酶(NSE)]检测联合应用对肺癌的诊断价值.方法 对350例临床怀疑为肺癌患者的99mTc-MIBI亲肿瘤显像、多排螺旋CT、血清肿瘤标志物榆测资料进行回顾性分析,总结每种方法及三种方法联合应用诊断肺癌的灵敏度、特异度及正确度.350例患者分别经纤维支气管镜检查、手术治疗及穿刺活组织枪查、病理最后证实200例为肺癌患者.结果 三种检查方法诊断肺癌的灵敏度、特异度及正确度分别为74.5%(149/200)、68.7%(103/150)、72.0%(252/350),97.5%(195/200)、42.7%(64/150)、74.0%(259/350)及62.5%(125/200)、28.7%(43/150)、48.0%(168/350).三法联合应用为99.5%(199/200)、82.0%(123/150)、92.0%(322/350).结论 上述三种检查方法对肺癌诊断各有其独特的重要的临床价值,三种方法联合应用诊断价值更大.%Objective To explore the clinical value of the combination of three methods including 99mTc-MIBI imaging, multi-slice spiral CT and tumor markers [carcinoembryonic antigen(CEA),cytokeretin19 fragment(CYFRA21-1), neuron-specific enolase(NSE)] measurement for diagnosis of lung cancer. Methods 350 patients were performed 99mTc-MIBl imaging, multi-slice spiral CT and measurements of tumor markers apart within one week. 200 patients were proved by pathology lung cancer in all 350 patients. Results The sensitivity, specificity and accuracy obtained with the three methods and combination of three methods were 74.5 %(149/200), 68.7 %(103/150), 72.0 %(252/350);97.5 %(195/200), 42.7 %(64/150), 74.0 %(259/350);62.5 %(125/200), 28.7 %(43/150), 48.0 %(168/350) and 99.5 %(199/200), 82.0 %(123/150), 92.0 %(322/350),respectively. Conclusion The results imply that three methods has its distinct important clinical value in diagnosis of lung cancer respectively

  12. Effect of cryoablation sequential chemotherapy on patients with advanced non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    Shu-Hui Yao

    2016-01-01

    Objective:To evaluate the effect of cryoablation sequential chemotherapy on patients with advanced non-small cell lung cancer.Methods:A total of 39 cases with advanced non-small cell lung cancer who received cryoablation sequential chemotherapy and 39 cases with advanced non-small cell lung cancer who received chemotherapy alone were selected and enrolled in sequential group and control group, disease progression and survival of two groups were followed up, and contents of tumor markers and angiogenesis molecules in serum as well as contents of T-lymphocyte subsets in peripheral blood were detected.Results:Progression-free survival and median overall survival (mOS) of sequential group were longer than those of control group, and cumulative cases of tumor progression at various points in time were significantly less than those of control group (P<0.05); 1 month after treatment, serum tumor markers CEA, CYFRA21-1 and NSE contents, serum angiogenesis molecules PCDGF, VEGF and HDGF contents as well as CD3+CD4-CD8+CD28-T cell content in peripheral blood of sequential group were significantly lower than those of control group (P<0.05), and contents of CD3+CD4+CD8-T cell and CD3+CD4-CD8+CD28+T cell in peripheral blood were higher than those of control group (P<0.05).Conclusions:Cryoablation sequential chemotherapy can improve the prognosis of patients with advanced non-small cell lung cancer, delay disease progression, prolong survival time, inhibit angiogenesis and improve immune function.

  13. Svensk korttegner trak Europas grænse

    DEFF Research Database (Denmark)

    Østergaard, Uffe

    2015-01-01

    Historisk set. At Europa slutter ved Ural var et vilkårligt valg til et politisk formål på et bestemt tidspunkt i historien. Men siden Moskva-fyrstendømmet i 1552 ekspanderede ind i Sibirien, har Rusland både været europæisk og asiatisk.......Historisk set. At Europa slutter ved Ural var et vilkårligt valg til et politisk formål på et bestemt tidspunkt i historien. Men siden Moskva-fyrstendømmet i 1552 ekspanderede ind i Sibirien, har Rusland både været europæisk og asiatisk....

  14. Effect of Remote Ischemic Preconditioning on Serum S100-β Protein and NSE in Pediatric Patients with Congenital Heart Disease Undergoing Cardiac Surgery by Cardiopulmonary Bypass%远隔缺血时处理对体外循环先心病手术患儿血清S100-β蛋白和NSE水平的影响

    Institute of Scientific and Technical Information of China (English)

    聂晓舟; 邓恢伟; 潘道波; 周爱国

    2014-01-01

    [目的]观察远隔缺血时处理对先心病患儿体外循环(CPB)心脏手术后血清S100 β蛋白和神经元特异性烯醇化酶(NSE)的影响.[方法]择期CPB下行先心病手术患儿60例,随机分为远隔缺血时处理组(R组)和对照组(C组),每组30例.R组在主动脉阻断同时对患者右下肢实施3周期4 min缺血/4 min再灌注处理;C组不进行该处理.两组分别于术前(T0)、CPB结束即刻(T1)、CPB结束后5 h(T2)、12 h(T3)、24 h(T4)5个时间点检测血清S100-β蛋白和NSE水平.[结果]两组患儿血清S100-β蛋白和NSE水平在CPB后均先逐渐升高,在CPB后5h达高峰,然后逐渐下降,在CPB后24 h恢复至术前水平.与T0相比,两组患儿血清S100-β蛋白和NSE水平在T1~3明显升高(P<0.05);与C组相比,R组患儿血清S100-β蛋白和NSE水平在T1~3较低(P <0.05).[结论]远隔缺血时处理能抑制CPB下先心病手术患儿S100-β蛋白和NSE的释放,对CPB后脑损伤可能有一定的保护作用.

  15. 晚期肺鳞癌患者肿瘤标志物测定的临床分析%Clinical Signiifcance of Tumor Marker Detection in Patients with Advanced Squamous Cell Carcimoma of the Lung

    Institute of Scientific and Technical Information of China (English)

    梁平; 李峻岭

    2016-01-01

    Background and objective Due to it's concealment and no obvious symptoms, lung squamous carcimoma otfen has advanced disease when diagnosed. hTe aims of this study were to describe the characteristics of the disease, to evaluate the clinical importance of detection of multiple tumor markers in patients with squamous cell carci-noma of the lung.Methods hTe characteristics of all patients with advanced squamous cell lung cancer treated in Beijing Cancer Hospital of Chinese Academy of Medical Sciences during Jan. 2011 to Dec. 2015 were identiifed by cases review-ing and data extracting. hTe characteristics, detection levels and sensitivity of multiple tumor makers among patients were described.Results hTe 260 patients were treated with mean age of (59.4±9.2) years, 85.8% (n=223) of them were male, 14.2% (n=37) of them were female. 78.1% (n=203) of all were smokers and 3.1% (n=8) of patients had family history of tumor. hTe positive rate of cytokerantin 19 fragment (CYFAR21-1) was 71.2%, which was the highest among ifve tumor markers. hTe ifve tumor markers median level had no statistical signiifcance between different tumor (T) stages and node (N) stages (allP>0.05), only the positive rate of SCC had statistical signiifcance between different T stages (P=0.035). hTe combination measurement of CYFRA21-1+carcinogen-embryonic antigen (CEA), CYFRA21-1+CEA+cancer antigen (CA125), CA125+CYFAR21-1+CEA+neuron speciifc enolase (NSE), and CA125+CYFAR21-1+NSE+CEA+squamous cell carcinoma antigen (SCC) were better and had higher clinical values, the positive rates were 82.7%, 84.6%, 85.0% and 86.2%, respectively.Conclusion hTe positive rate of CYFAR21-1 was the highest and the sensitivity of single test of ifve tumor markers was low, the combination of multiple tumor markers increased the sensitivity of diagnosis of SQCLC, the combination of CA125, CYFAR21-1 and CEA was the best choice.%背景与目的肺鳞癌由于发病隐匿,早期无明显症状,往往到晚期才得

  16. Dynamic Observation of the Effect of Chemotherapeutic Agents on Plasma ET, CYPRA21-1, CEA in Lung Cancer Patients%化疗药物对肺癌患者血浆ET、CYFFRA21-1、CEA影响的观察

    Institute of Scientific and Technical Information of China (English)

    刘波; 宋恕平; 陈阵; 盛立军; 周登光; 艾斌; 郭君; 宋冬

    2002-01-01

    目的:检测肺癌患者血浆内皮素(Endothlin,ET)、细胞角蛋白19片段(Cytokeration Fragment Antigen221-1,CYFRA21-1)、癌胚抗原(Carcinoembryonic antign,CEA)水平及化疗对它们的影响,以探讨血浆ET、CYFRA21-1、CEA在肺癌发病及预后过程中的作用.方法:应用放射免疫分析法检测29例正常人及68例肺癌患者化疗前、化疗1个周期后、化疗2个周期后血浆ET、CYFRA221-1、CEA测值,并进行对比分析.结果:化疗前非小细胞肺癌患者血浆ET、CYFRA21-1测值高于正常对照组(P<0.01),化疗后ET、CYFRA21-1、CEA与化疗前比较差异有显著性(P<0.05或P<0.01);而小细胞肺癌患者ET、CYFRA21-1、CEA测值则无明显变化(P>0.05).化疗前Ⅱ~Ⅳ期肺癌患者ET、CYFRA21-1.测值高于正常对照组(P<0.01),Ⅱ、Ⅲ、Ⅳ各期间ET有显著性差异(P<0.05),而CYFRA21-1、CEA测值差异无显著性(P>0.05).提示血浆ET的水平与非小细胞肺癌的临床分期相平行,病期越晚者血浆ET测值越高.血浆ET、CYFRA21-1、CEA的水平与疗效呈正相关(P<0.05).血浆ET测值受心脏病、吸烟、性别因素的影响,血浆CEA测值受吸烟因素的影响.结论:ET、CYFRA21-1、CEA可能与非小细胞肺癌有关联,化疗可使ET、CYFR21-1.、CEA水平降低,而对小细胞肺癌则无明显影响.肺癌患者ET、CYFRA21-1、CEA的升高有可能成为进展期非小细胞肺癌的肿瘤标志物,ET、CYFRA21-1、CEA可对判断肿瘤疗效有一定作用.但应考虑心脏病、吸烟、性别因素对ET、CEA的影响.

  17. A magnetic nanoparticle-based time-resolved fluoroimmunoassay for determination of the cytokeratin 19 fragment in human serum.

    Science.gov (United States)

    Lin, Guanfeng; Liu, Tiancai; Hou, Jingyuan; Ren, Zhiqi; Zhou, Jianwei; Liang, Qianni; Chen, Zhenhua; Dong, Wenqi; Wu, Yingsong

    2015-03-01

    A sensitive, rapid and novel measurement method for cytokeratin 19 fragment (CYFRA 21-1) in human serum by magnetic particle-based time-resolved fluoroimmunoassay (TRFIA) is described. Built on a sandwich-type immunoassay format, analytes in samples were captured by one monoclonal antibody coating onto the surface of magnetic beads and "sandwiched" by another monoclonal antibody labeled with europium chelates. The coefficient variations of the method were lower than 7%, and the recoveries were in the range of 90-110% for serum samples. The lower limit of quantitation of the present method for CYFRA 21-1 was 0.78 ng/ml. The correlation coefficient of CYFRA 21-1 values obtained by our novel TRFIA and CLIA was 0.980. The present novel TRFIA demonstrated high sensitivity, wider effective detection range and excellent reproducibility for determination of CYFRA 21-1 can be useful for early screening and prognosis evaluation of patients with non-small cell lung cancer.

  18. Clinical effect of laparoscopic-assisted radical gastrectomy in treatment of advanced gastric cancer and its effects on serum tumor markers and high sensitive C reactive protein in elderly patients%腹腔镜辅助胃癌根治术治疗老年进展期胃癌的临床效果及对血清肿瘤标志物和高敏C反应蛋白的影响

    Institute of Scientific and Technical Information of China (English)

    赵昕波; 游红勇; 李兵; 陈智敏; 刘东涛

    2015-01-01

    Objective To investigate the long-term clinical efficiency of laparoscopic-assisted radical gastrectomy in the treatment of advanced gastric cancer and the effects on the serum tumor markers and high sensitive C reactive protein (hs-CRP) in elderly patients.Methods Totally 86 elderly patients of advanced gastric cancer from August 2008 to June 2012 were enrolled and divided into laparoscopic group (43 cases) and laparotomy group (43 cases).The long-term therapeutic efficiency was evaluated.The levels of serum tumor markers including carcino embryonic antigen (CEA),neuron-specific enolase (NSE),cytokeratin fragment antigen 21-1 (CYFRA21-1) carbohydrate antigen 125 (CA125) and carbohydrate antigen 19-9(CA19-9),hs-CRP,blood coagulation index before and after operation were measured and compared between two groups.Results The operation time and the number of dissected lymph node showed no significant difference between two groups (P > 0.05);bleeding,using times of analgesic,gastrointestinal recovery time,hospitalization time and incidence of adverse reactions in laparoscopic group were significantly lower than those in traditional laparotomy group [(89 ±19) mlvs (122±25) ml,(0.68 ±0.28) timesvs (2.19 ±0.53) times,(2.6 ±0.4) d vs (3.9 ±0.5) d,(9.1 ±1.8) d vs (12.6 ±2.4) d,14.0% (6/43) vs 39.5% (17/43),P<0.05];overall survival of laparoscopic group and laparotomy group had no significant difference [10.2 months (95% CI:8.211-13.089) and 9.7 months (95% CI:5.804-10.596) (x2 =3.481,P =0.062).The serum tumor markers,coagulation indices and hs-CRP showed no significant differences between two groups before operation (P > 0.05);they were all improved after operation.The levels of CEA,NSE,CA125,CA19-9 and hs-CRP in laparoscopic group were all significantly lower compared with those in laparotomy group after operation [(10.1 ± 2.6) μg/L vs (18.8 ±5.2) μ.g/L,(14±3) μg/L vs (22±7) μg/L,(31 ±7) kU/L vs (45 ±7) kU/L,(28 ±7) kU/L vs (47±17) kU/L,(2

  19. Urine and serum concentrations of Cytokeratin 19 in preeclampsia.

    Science.gov (United States)

    Kuessel, Lorenz; Wild, Julia; Haslacher, Helmuth; Perkmann, Thomas; Ristl, Robin; Zeisler, Harald; Schmid, Maximilian

    2014-10-01

    To evaluate the usefulness of Cytokeratin 19 as biomarker for the diagnosis of preeclampsia. Cytokeratin 19 protein fragment CYFRA 21-1 was measured by means of electrochemiluminescence immunoassays in urine and serum samples of 32 women with preeclampsia and 32 samples of normotensive healthy singleton pregnancies at random, matched for gestational age, as controls. The median serum concentrations of CYFRA 21-1 in controls and women with preeclampsia were 2.4 (range 1.3-6.6)ng/mL and 4.4 (range 2.1-16.2)ng/mL, respectively (ppreeclampsia were 13.7 (range 0.7-441.4)ng/mL and 11.8 (range 1.5-338.6)ng/mL, respectively (p=0.629). Calculation of a ROC curve to study the use of serum CYFRA 21-1 concentration as a predictor of preeclampsia revealed cut-off points with the highest sum of specificity and sensitivity of 3.2ng/mL, leading to specificity of 75% and sensitivity of 84%. A similar curve calculated for CYFRA 21-1 in urine showed an area under the curve of 0.536 meaning no predictive power. The correlation between urinary excretion of protein in 24h and serum concentrations of CYFRA 21-1 in the case group was r=0.26, which is not significant (p=0.258). The correlation between proteinuria and urine values of CYFRA 21-1 was r=0.10, which also is not significant (p=0.666). Serum levels of Cytokeratin 19 fragment are increased in women with preeclampsia. However this does not result in a significant difference in CYFRA 21-1 levels in maternal urine. Thus Cytokeratin 19 fragment may prove to be a valuable biomarker for preeclampsia in serum but not urine. We propose further longitudinal studies to investigate the role of Cytokeratin 19 in maternal serum of women with preeclampsia. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  20. 呼气冷凝液中3种肿瘤标志物联合检测在肺癌诊断中的价值研究%Value of combined detection with three tumor markers in exhaled breath condensate on the diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    马伦; 詹伟杰; 廖绍宗; 潘赛英

    2013-01-01

    目的:对呼气冷凝液(EBC )中3种肿瘤标志物进行联合检测,研究其在肺癌诊断中的临床价值,探讨该方法在临床应用的可能性,为今后的肺癌诊断和治疗效果评估提供可靠的参考依据。方法选择2011年1月15日至2012年12月15日该院肿瘤科收治的肺癌患者30例作为肺癌组,另抽取同期健康体检者30例作为健康对照组,对肺癌组患者治疗前后和健康对照组进行 EBC及血清中癌胚抗原(CEA )、细胞角蛋白19的可溶性片段(CYFRA21-1)、血管内皮生长因子(VEGF)水平检测,并对比分析检测结果。采用标准 EBC 收集器收集 EBC , CEA、CYFRA21-1采用化学发光法,VEGF用酶联免疫吸附法测定。结果肺癌组患者EBC及血清中CEA、CY-FRA21-1、VEGF检测水平明显高于对照组(P<0.05),化疗后CEA、CYFRA21-1、VEGF水平较治疗前显著降低(P<0.05),EBC中CEA、CYFRA21-1、VEGF水平较血清中低(P<0.05)。结论对EBC中CEA、CYFRA21-1、V EG F水平进行检测对于肺癌的诊断、病理分型和疗效判断均具有重要的参考价值,EBC检测结果与血清结果联合可实现相互补充,提高阳性率,值得关注。%Objective To combinedly detect three tumor markers in exhaled breath condensate ,analyze the clinical value on diagnosis of lung cancer ,discuss the possibility of the method applied in the clinic ,and provide a reli-able basis for the lung cancer diagnosis and treatment in the future assessment .Methods 30 cases with lung cancer from January 15 ,2011 to December 15 ,2012 in our hospital were selected as lung cancer group ,and the other 30 healthy people were selected as control group .The CEA ,CYFRA21-1 and VEGF levels in exhaled breath condensate and serum were detected in lung cancer group and control group ,and the detection results were comparatively ana-lyzed .Results The CEA ,CYFRA21-1 and VEGF levels in lung cancer group were

  1. Comparison of 5 Kinds of Serum Tumor Markers in the Clinical Effect of Combined Detection for the Diagnosis and Treatment of Lung Cancer%比较5种血清肿瘤标志物联合检测对肺癌诊断和治疗的临床效果

    Institute of Scientific and Technical Information of China (English)

    江涛

    2014-01-01

    Objective:To analyze 5 kinds of serum tumor markers for clinical effect of combined detection for the diagnosis and treatment of lung cancer.Method:By enzyme-linked immunoassay for 50 patients with lung cancer and 50 patients with lung benign disease serum tumor markers were detected,the main test items of serum neuron specific enolization enzyme(NSE)and cytokeratin 19 fragment(Cy-fra21-1),carbohydrate antigen 199 (CA199),carbohydrate antigen 125(CAl25)and carcinoembryonic antigen(CEA),and 5 kinds of serum tumor markers for clinical effect of combined detection for the diagnosis and treatment of lung cancer were investigated and analyzed.Result:The levels of five kinds of serum tumor markers in patients with lung cancer were higher than the detection level of patients with lung benign disease,the differences were statistically significant(P0.05),two level detection of CA123 and Cy-fra21-1 in patients with lung cancer of different pathological type,the differences were statistically significant(P0.05).The five kinds of tumor markers combined detection sensitivity,accuracy and specific degree 92.0%,89.0%and 88.0%respectively,were superior to single detection of tumor markers,the differences were statistically significant(P0.05),不同病理类型的肺癌患者的CA123和Cy-fra21-1两种检测水平比较差异均有统计学意义(P0.05)。而5种肿瘤标志物联合检测的灵敏度、准确度和特异度分别为92.0%、89.0%、88.0%,均明显优于单个肿瘤标志物的检测,比较差异均有统计学意义(P<0.05)。结论:血清肿瘤标志物的联合检测可显著提高肺癌患者的灵敏度和准确度,对肺癌患者的诊断和治疗有显著的临床意义。

  2. Establishment and Characterization of a Novel Chinese Human Lung Adenocarcinoma Cell Line CPA-Yang2 in Immunodeficient Mice

    Directory of Open Access Journals (Sweden)

    Shunfang YANG

    2009-10-01

    Full Text Available Background and objective The recurrence, metastasis and multidrug resistance (MDR in lung cancer are the tough problems worldwide. This study was to establish a novel chinese lung adenocarcinoma cell line with high metastasis potential for exploring the mechanism of reccurrence, development and MDR in lung cancer. Methods The cell came from the abdominal dropsy of a fifty-six years old female patient with lung adenocarcinoma and the tumor markers CA125, CYFRA21-1, CEA, NSE were detected to be higher secretion by radioimmunoassay in the abdominal dropsy. Tumorigenicity of immunodeficient mice was confirmed in 8th passage. The cell growth curve was mapped. Analysis of chromosome karyotype was tested. The gene expression was measured by real-time quantitative PCR. Results The tumorigenesis rate started at 8th passage in 3/10 immunodeficient mice via subcutaneously and the fully tumorigenicity was at 11th passage as well as later passages. Under the microscope, the cell showed oval-shap and adherence. The chromosome karyotype analysis of the cells was sub-triploid. Approximately 1×106 and 1.5×106 cancerous cells were injected into left cardiac ventricle and tail vein of immunodeficient mice respectively. The results showed multiorgan metastasis in the mice after three-four weeks, including mandible, scapula, humerus, vertebral column, femur, rib and brain, liver, adrenal gland, pulmonary in the mice after inoculation. The bone metastasis rate was 100% in the tumor bearing mice by bone scintigraphy and pathology. Quantitative real-time PCR was used to examined and compared with SPC-A-1 lung adenocarcinoma, ESM1, VEGF-C, IL-6, IL-8, AR genes were overexpressed. The novel cell was named CPA-Yang2. Conclusion The characteristics of novel strain CPA-Yang2 is a highly metastasis cell line of Chinese lung adenocarcinoma. It has stable traits, highly metastasis ability and maybe is a MDR lung cancerous cell line. Of course, it’s a good experimental

  3. The role of squamous cell carcinoma antigen as a prognostic and predictive factor in carcinoma of uterine cervix

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Bae Kwon; Choi, Doo Ho; Huh, Seung Jae; Park, Won; Bae, Duk Soo; Kim, Byoung Gie [Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2011-09-15

    Although the role of squamous cell carcinoma antigen (SCC-Ag) as a predictive and prognostic factor for uterine cervical cancer has been identified ed in previous studies, 1) the effective patient group of screening for recurrence with SCC-Ag, 2) the relationship between SCC-Ag and recurrence site, and 3) the relationship between the change of SCC-Ag and treatment outcome or recurrence have not been described. The study included 506 patients with histologically proven uterine cervical cancer between January 1994 and December 2010. We determining the serum SCC-Ag level before treatment and after treatment, and conducted a retrospective review of the patients' records. We evaluated the sensitivity and specificity of SCC-Ag for the detection of tumor recurrence by comparing biochemical recurrence with clinical recurrence. The pretreatment SCC-Ag level and the proportion of patients over 1.5 ng/mL were higher in poor prognostic patient group. In the univariate and multivariate analysis, pretreatment SCC-Ag showed a statistically significant correlation with tumor size, International Federation of Gynecology and Obstetrics (FIGO) stage, pathology. In patients with biochemical recurrence vs. those without, 5-year DFS and OS were 27.6 vs. 92.7% (p{<=}0.001) and 53.7 vs. 92.5% (p{<=}0.001), respectively. Our study reconfirmed the known function of pretreatment SCC-Ag, but could not confirm the function of biochemical response as a predictive factor for treatment and as a prognostic factor. There was no statistically significant relationship between SCC-Ag level and recurrence site. We confirmed the role of SCC-Ag as a follow-up tool for recurrence of disease and which patient groups SCC-Ag was more useful for.

  4. 探讨5种血清肿瘤标志物在肺癌辅助诊断中的应用价值%Discussion on 5 kinds of Serum Clinical Value of Tumor Markers in the Diagnosis of Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    邓小妹

    2015-01-01

    目的:研究5种血清肿瘤标志物在肺癌辅助诊断中的应用价值。方法选取我院在2010年11月~2013年11月收治的62例肺癌患者为研究对象,选取同期收治的肺良性疾病患者62例作为对照,对所有患者的5种血清肿瘤标志物进行检测,检测项目包括NSE(血清神经元特异性烯醇化酶)、CEA(癌胚抗原)、CA125(糖类抗原125)、CA199(糖类癌抗原199)、Cy-fra21-1(细胞角蛋白19片段)。结果肺癌患者在NSE、CEA、CA125、CA199、Cy-fra21-15项指标上均低于肺部良性疾病患者,对比具有统计学意义(<0.05)。5种血清肿瘤标志物联合检测的灵敏度为95.2%,特异度为82.3%,准确度为87.6%。结论经研究得知,通过对患者5种血清肿瘤标志物进行检测,有利于提高肺癌疾病的诊断正确率,值得临床推广应用。%Objective To study the 5 kinds of serum tumor markers in the diagnosis of lung cancer auxiliary application value. Methods Select our hospital in November 2010 to November 2013, 62 cases of patients with lung cancer as the research object, during the same period of 62 patients with lung benign disease were selected as control, for al 5 kinds of serum tumor markers in patients with testing, test items include NSE serum neuron specificity enolization (enzyme) and CEA (carcinoembryonic antigen), CA125 (carbohydrate antigen 125), CA199 cancer antigen 199 (sugar), Cy-fra21-1 (cytokeratin 19 fragment). Results NSE in patients with lung cancer, CEA, CA125, CA199 and Cy- fra21-15 indicators are lower than in patients with lung benign disease, compared with statistical significance ( < 0.05). Five kinds of serum tumor markers combined detection sensitivity of 95.2%, 82.3%, the accuracy is 87.6%. Conclusion The study, based on the five kinds of serum tumor markers in patients with testing, to improve the diagnostic accuracy of lung disease, worthy of clinical popularization and application.

  5. 血清肿瘤标志物联合检测在肺癌诊断中的临床应用%Clinical application of combined detection of serum tumor markers in diagnosis of lung cancer

    Institute of Scientific and Technical Information of China (English)

    张静; 贾继敏

    2013-01-01

    Objective To investigate the significance of combined detection of serum tumor markers in the diagnosis of lung cancer,and to select the most sensitive serum tumor markers.Methods Enzyme-linked immunosorbent assay was used to detect the serum tumor markers of 62 lung cancer patients and 62 healthy persons,who were neuron specific enolase enzyme (NES),carbohydrate antigen 125 (CA 125),cytokeratin 19 (CYFRA21-1) and carcinoembryonic antigen(CEA).Results The level of serum CEA,CA125,CYFRA21-1,and NES of the lung cancer group were all higher than those of the control group (all P < 0.05).Among the four kinds of tumor markers,CA125 had the highest sensitivity(59.7%),which was followed by CEA(53.2%).The sensitivity of the four kinds of joint detection was 87.1%,the highest sensitivity of three markers joint detection was CEA + CA125 + CYFRA21-1 (87.1%),while that of the two kinds of joint detection was CEA + CA125 (75.8%).Conclusion There are high clinical significance in the diagnosis of lung cancer with four kinds of serum tumor markers,CEA + CA125 + CYFRA21-1 can be used as the best markers for joint detection of lung cancer with high sensitivity.%目的 探讨血清肿瘤标志物联合检测在肺癌诊断中的意义,并选出敏感性最高的血清肿瘤标志物组合.方法 应用酶联免疫吸附法检测62例肺癌患者和62例健康体检者血清中神经元特异性烯醇化酶(NES)、糖链抗原125(CA125)、细胞角蛋白19(CYFRA21-1)及癌胚抗原(CEA)的含量水平.结果 肺癌组患者血清CEA、CA125、CYFRA21-1及NES水平均高于对照组(均P<0.05);在4种肿瘤标志物中,CA125的敏感性最高(59.7%),其次为CEA(53.2%);4种标志物联合检测的敏感性为87.1%,3种标志物联合检测中,以CEA+ CA125+ CYFRA21-1敏感性最高,为85.5%,2种标志物联合检测中CEA+ CA125敏感性最高,为75.8%.结论 4种血清肿瘤标志物对于肺癌的辅助诊断有一定的临床意义,CEA、CA125、CYFRA21-1

  6. 肿瘤标志物在肺癌患者中的表达及其临床意义%The expression of tumor markers in lung cancer patients and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    梁民勇

    2011-01-01

    目的:探讨血清肿瘤标志物CA50、CEA、Cyfra21-1和scc在肺癌诊断中的价值.方法:采用化学发光法检测140例肺癌患者、60例肺良性病变患者及80例健康体检者血清CASO、CEA、cyfra21-1和Scc的含量,并进行统计学分析.结果:肺癌患者CA50、CEA、Cyfra21-1和Scc的阳性率分别为45.7%、65.7%、58.5%和59.3%,显著高于肺部良性病变患者和健康对照组(P<0.05).CASO、CEA、Cyfra21-1和Scc小细胞肺癌患者较非小细胞肺癌患者表达水平低.CA50和CEA在肺癌高表达;而cyfh12I-1在肺鳞癌高表达.结论:血清CA50、CEA、Cyfra-1和Scc检测对于肺癌诊断敏感性和特异性强,联合检测有助于对肺癌的诊治以及病情的判断分析.

  7. Pathological features of esophageal cancer patients with different blood concentrations of tumor markers in comparison%不同病理特征的食管癌患者外周血肿瘤标记物浓度变化比较

    Institute of Scientific and Technical Information of China (English)

    张磊

    2011-01-01

    目的:比较不同病理特征的食管癌患者外周血肿瘤标记物浓度变化.方法:连续选择接受手术治疗的食管癌患者49例,治疗前进行血清癌胚抗原(CEA)和角蛋白19片段(Cyfra21-1)标本采集,并根据手术切除标本的病理特征进行分组,比较肿瘤标记物浓度变化.结果:具有中、低程度分化、浸润肌层、淋巴结转移和瘤体≥5 cm病理特点的食管癌患者血清CEA和Cyfra21-1浓度值均明显高于高分化、浸润外膜无淋巴结转移和瘤体<5 cm患者(P均<0.05或0.01).结论:食管癌患者的肿瘤细胞分化程度、瘤体大小、浸润程度及有无淋巴结转移等病理表现与外周血CEA和Cyfra21-1浓度值密切相关.

  8. A trip——A Teaching Plan for Unit 1,Module 10 NSE Book 10

    Institute of Scientific and Technical Information of China (English)

    卢晓霞

    2006-01-01

    @@ Topic: Talking about a trip Instructional objectives: After the lesson, the students will be able to 1) say the dialogue and say the chant "I am getting ready for my trip"correctly; 2) talk about a trip, using the framework given: Who? Where?When? What? How?

  9. The causes of bullying: results from the National Survey of School Health (PeNSE

    Directory of Open Access Journals (Sweden)

    Wanderlei Abadio de Oliveira

    2015-04-01

    Full Text Available Objective: to identify the characteristics and reasons reported by Brazilian students for school bullying. Method: this cross-sectional study uses data from an epidemiological survey (National Survey of School Health conducted in 2012. A total of 109,104 9th grade students from private and public schools participated. Data were collected through a self-applied questionnaire and the analysis was performed using SPSS, version 20, Complex Samples Module. Results: the prevalence of bullying was 7.2%, most frequently affecting Afro-descendant or indigenous younger boys, whose mothers were characterized by low levels of education. In regard to the reasons/causes of bullying, 51.2% did not specify; the second highest frequency of victimization was related to body appearance (18.6%; followed by facial appearance (16.2%; race/color (6.8%; sexual orientation 2.9%; religion 2.5%; and region of origin 1.7%. The results are similar to those found in other sociocultural contexts. Conclusion: the problem belongs to the health field because it gathers aspects that determine the students' health-disease-care continuum.

  10. The causes of bullying: results from the National Survey of School Health (PeNSE)

    Science.gov (United States)

    de Oliveira, Wanderlei Abadio; Silva, Marta Angélica Iossi; de Mello, Flávia Carvalho Malta; Porto, Denise Lopes; Yoshinaga, Andréa Cristina Mariano; Malta, Deborah Carvalho

    2015-01-01

    Objective: to identify the characteristics and reasons reported by Brazilian students for school bullying. Method: this cross-sectional study uses data from an epidemiological survey (National Survey of School Health) conducted in 2012. A total of 109,104 9th grade students from private and public schools participated. Data were collected through a self-applied questionnaire and the analysis was performed using SPSS, version 20, Complex Samples Module. Results: the prevalence of bullying was 7.2%, most frequently affecting Afro-descendant or indigenous younger boys, whose mothers were characterized by low levels of education. In regard to the reasons/causes of bullying, 51.2% did not specify; the second highest frequency of victimization was related to body appearance (18.6%); followed by facial appearance (16.2%); race/color (6.8%); sexual orientation 2.9%; religion 2.5%; and region of origin 1.7%. The results are similar to those found in other sociocultural contexts. Conclusion: the problem belongs to the health field because it gathers aspects that determine the students' health-disease-care continuum. PMID:26039298

  11. 网络安全漏洞分析系统NSE

    Institute of Scientific and Technical Information of China (English)

    大秋

    2006-01-01

    网络安全漏洞分析系统NSE(手持式)由国家反计算机入侵和防病毒研究中心研发.运行于夏普CECSL7500C掌上电脑.是目前国内独有的基于夏普Zaurus系列PDAS的网络安全漏洞分析系统。该产品既可实现学习、生活、工作等各方面的数字助理功能.又拥有强大的网络安全漏洞分析系统功能.其漏洞扫描核心部分与功能强大的NSE台式机漏洞分析系统的核心部分完全相同。

  12. New software engineering paradigm based on complexity science an introduction to NSE

    CERN Document Server

    Xiong, Jay

    2011-01-01

    This book describes a revolution in software engineering - the Nonlinear Software Engineering paradigm, which complies with the essential principles of complexity science and can help double productivity, halve costs and reduce defects in software products.

  13. The causes of bullying: results from the National Survey of School Health (PeNSE).

    Science.gov (United States)

    Oliveira, Wanderlei Abadio de; Silva, Marta Angélica Iossi; Mello, Flávia Carvalho Malta de; Porto, Denise Lopes; Yoshinaga, Andréa Cristina Mariano; Malta, Deborah Carvalho

    2015-04-14

    to identify the characteristics and reasons reported by Brazilian students for school bullying. this cross-sectional study uses data from an epidemiological survey (National Survey of School Health) conducted in 2012. A total of 109,104 9th grade students from private and public schools participated. Data were collected through a self-applied questionnaire and the analysis was performed using SPSS, version 20, Complex Samples Module. the prevalence of bullying was 7.2%, most frequently affecting Afro-descendant or indigenous younger boys, whose mothers were characterized by low levels of education. In regard to the reasons/causes of bullying, 51.2% did not specify; the second highest frequency of victimization was related to body appearance (18.6%); followed by facial appearance (16.2%); race/color (6.8%); sexual orientation 2.9%; religion 2.5%; and region of origin 1.7%. The results are similar to those found in other sociocultural contexts. the problem belongs to the health field because it gathers aspects that determine the students' health-disease-care continuum.

  14. Måling af prokalcitonin kan muligvis begrænse antibiotikaforbruget ved pneumoni

    DEFF Research Database (Denmark)

    Berg, Peter; Lindhardt, Bjarne Ørskov

    2012-01-01

    with promising reductions in antibiotic use and treatment duration, but more research is needed in order to draw a final conclusion. Lack of objective diagnostic criteria, methodological challenges in research, a future focus on primary care, and the need for a cost-benefit analysis should be considered....

  15. Effekt af lavere promillegrænse for unge motorførere

    DEFF Research Database (Denmark)

    Sørensen, Poul Lyk; Kærup, Simon

    2012-01-01

    Der gælder særlige lave promillegrænser for nyuddannede motorførere i følgende lande i Europa: Grækenland, Luxemburg, Holland, Letland, Slovenien, Spanien, Tyskland og Østrig. Desuden findes dette i Canada, USA og Australien, men indtil nu har ikke alle lande evalueret effekten. I nedenstående ar...

  16. HISTORICAL AND IMPLIED VOLATILITY: AN INVESTIGATION INTO NSE NIFTY FUTURES AND OPTIONS

    Directory of Open Access Journals (Sweden)

    N R Parasuraman

    2011-10-01

    Full Text Available The broad objective of the paper is to have an understanding of the movement of volatility over a fair period in respect of the market portfolio. Also, it enables an understanding on how divergent the implied volatility has been from this estimate. It uses Volatility Cone, Volatility Smile and Volatility Surface as the parameters. The study takes different rolling periods percentiles of volatility. Hoadley Options Calculator is used for calculation and analysis purpose. The study empirically proves that there is a clear reversion to the mean as indicated by the volatility cone. The study of volatility smiles in respect of NIFTY options throws up different patterns. The Garch (1.1 model reveals that historical volatility for the period from 2004 to 2004 and for the year 2009 were estimated. Interestingly, but not totally surprisingly, the average implied volatility of calls and puts on Nifty during the period January to March 2010 showed differences.

  17. Måling af prokalcitonin kan muligvis begrænse antibiotikaforbruget ved pneumoni

    DEFF Research Database (Denmark)

    Berg, Peter; Lindhardt, Bjarne Ørskov

    2012-01-01

    Reduction of antimicrobial resistance in microorganisms is imperative. Pneumonia is important in this matter because of its high incidence, subjective diagnostic criteria, and variations in aetiology. Research has focused on the use of a procalcitonin-guided algorithm for antimicrobial stewardship...... with promising reductions in antibiotic use and treatment duration, but more research is needed in order to draw a final conclusion. Lack of objective diagnostic criteria, methodological challenges in research, a future focus on primary care, and the need for a cost-benefit analysis should be considered....

  18. Low-dose spiral CT combined tumor markers for lung cancer screening high-risk groups%低剂量CT与4肿瘤标记物联合检测对肺癌高危人群筛查的研究

    Institute of Scientific and Technical Information of China (English)

    李丰章; 邹南安; 胡含明; 巫启恒; 欧阳良

    2015-01-01

    Objective To evaluate screening positive rate and specificity of low-dose spiral computed tomography ( LDCT) scanning joint 4 kinds of lung cancer tumor markers (multi-effect PTN,protein and neuron specific enzyme NSE,cell keratin pro-tein enolization Cyfra21-1 and carcinoembryonic antigen CEA) in detection of lung cancer at higher risk of early lung cancer. Methods Analysis and statistics of 2011-2013 LDCT scanning and lung cancer tumor markers combined screening high-risk group of test results,and comparing with conventional early lung cancer screening study result analysis. Results LDCT lung posi-tive detection rate with conventional dose CT scan imaging findings and pathological changes,no statistical difference(P>0.05),and radiation dose decreased obviously,it was regular doses of 6.38%(P<0.05);Four types of lung cancer tumor markers combined de-tection sensitivity about 95%,specificity about 80% of lung cancer,was of great significance for early diagnosis of lung cancer,4 kinds of this experimental lung cancer tumor markers positive rate was 1.58%(6 cases),ultimately determine the early lung cancer was 1.05%(4 cases),LDCT to detect lesions positive rate was 12.1%in this group (46 cases),of which 8 cases of imaging diagnosis of early lung cancer (2.11%),and finally only 4 cases (1.05%) diagnosed with lung cancer early,imaging in the diagnosis of false positives. To participate in this study,a total of 759 people,of whom LDCT and tumor markers combined test group(379,female:379 people,average age 54.3 years,7 patients with copd;male:203,the average age of 57.6 years,26 patients with copd screening lung were 12.1%(46 people with lung CT positive performance,6 people joint inspection tumor markers were positive,4 cases were diagnosed with lung cancer). Conclusion LDCT two inspection methods and lung cancer tumor markers have complementary ad-vantages,early lung cancer detection rate was 1.05%,avoid single check false positive events,joint detection more conducive

  19. 新辅助化疗对局部晚期非小细胞肺癌肿瘤标记物和淋巴细胞亚群的影响%Effect of neoadjuvant chemotherapy on locally advanced non-small cell lung cancer tumor markers and lymphocyte subsets

    Institute of Scientific and Technical Information of China (English)

    王纯; 卢宏达

    2014-01-01

    目的:观察新辅助化疗对不同病理类型的局部晚期非小细胞肺癌(NSCLC )肿瘤标记物、淋巴细胞亚群的影响。方法选取该院新辅助化疗的局部晚期NSCLC患者为研究对象,共计40例,选取20例体检的健康人群为对照组,比较不同病理类型的NSCLC肿瘤标记物癌胚抗原(CEA)、糖类蛋白-125(CA-125)、细胞角蛋白19可溶性片段(CYFRA21-1)的水平以及淋巴细胞亚群CD3+、CD4+、CD8+比例。分析不同疗效的NSCLC患者肿瘤标记物水平及淋巴细胞亚群的差异。结果(1)腺癌、鳞癌患者CEA、CA-125和CYFRA21-1水平均显著高于健康人群(P<0.05);鳞癌患者CEA升高水平低于腺癌,而鳞癌CA-125和CYFRA21-1升高水平高于腺癌(P<0.05);相对于健康人群,腺癌、鳞癌患者CD3+、CD4+、CD4+/CD8+均显著降低,而CD8+升高(P<0.05);但鳞癌、腺癌患者CD3+、CD4+、CD8+、CD4+/CD8+差异无统计学意义(P>0.05)。(2)经2个周期化疗后,缓解和部分缓解共18例,稳定和进展共22例。新辅助化疗有效的鳞癌患者血清CA-125和CYFRA21-1水平显著降低(P<0.05),新辅助化疗有效的腺癌患者血清CEA 和 CYFRA21-1水平显著降低(P<0.05)。新辅助化疗有效鳞癌、腺癌患者CD3+、CD4+、CD4+/CD8+升高,CD8+降低。结论不同病理类型的晚期NSCLC患者血清肿瘤标记物表达水平具有一定的差异,血清标记物与淋巴细胞亚群的变化均可作为评价新辅助化疗疗效的指标。%Objective To observe and analyze the effect of neoadjuvant chemotherapy on locally advanced non-small cell lung cancer tumor markers and lymphocyte subsets of different pathological type .Methods A total number of 40 NSCLC patients which received neoadjuvant chemotherapy and 20 normal people were selected in our study .To compare the differences of CEA ,CA-125 , CYFRA21-1 and

  20. 食管癌新辅助放化疗患者血清肿瘤标记物水平动态变化及意义%Changes and significance of serum tumor markers in patients with esophageal carcinoma undergoing neoadjuvant chemoradiotherapy

    Institute of Scientific and Technical Information of China (English)

    李广旭; 宋平平; 贾慧; 李浩; 张曙光; 张百江

    2014-01-01

    目的:探讨血清肿瘤标记物水平动态变化判断食管癌新辅助放化疗患者疗效及预后的价值。方法对30例行新辅助放化疗食管癌切除术患者的血清细胞角化素蛋白片段19( CYFRA21-1)、癌胚抗原( CEA )及糖链抗原19-9(CA19-9)水平行动态观察,分析其变化与临床疗效及预后的关系。结果治疗前CEA阳性者中位生存期明显短于CEA阴性者,P<0.05;CA19-9阳性者中位生存期短于CA19-9阴性者(但因阳性例数过少,无法行统计学处理);CYFRA21-1阳性与阴性者中位生存期无统计学差异。治疗过程中标志物呈下降趋势者中位生存期长于、有效率高于呈上升趋势者,P均<0.05。结论对食管癌新辅助放化疗患者血清CYFRA21-1、CEA及CA19-9联合动态及变化趋势检测,可评价治疗效果及判断预后。%Objective To discuss the dynamic changes of serum CYFRA 21-1, carcinoembryonic antigen (CEA) and carbohydrate antigen ( CA) 19-9 levels before and after neoadjuvant chemoradiotherapy to evaluate its efficacy and prognosis in treatment of patients with esophageal carcinoma .Methods The dynamic changes in serum levels of cytokeratin 19 frag-ment(CYFRA21-1), CEA and CA19-9 were observed during treatment and after treatment in 30 cases of patients who re-ceived neoadjuvant chemoradiotherapy with esophageal cancer resection .The relationship between the changes and the clin-ical efficacy and prognosis was analyzed .Results The median survival time of CEA positive people was significantly shor-ter than that of negative ones before treatment (P<0.05).The median survival time of CA19-9 positive people was shorter than that of negative ones ( because the positive cases were few , it was unable to statistical processing ) , and the median survival period of CYFRA21-1 positive and negative patients was not statistically different .During treatment, their median survival of those patients whose markers

  1. Study on the Clinical Application Value of SP70 Antigen in Differential Diagnosis of Malignant Pleu-ral Effusion%SP70抗原在癌性胸腔积液鉴别诊断中的临床应用价值

    Institute of Scientific and Technical Information of China (English)

    张丹华

    2015-01-01

    目的:探讨SP70抗原检测在癌性胸腔积液鉴别诊断中的应用价值。方法选取2013年1月至2014年1月达州陆军医院收治的200例胸腔积液患者作为研究对象、其中100例癌性胸腔积液,100例非癌性胸腔积液。患者的胸腔积液均检测SP70抗原、癌胚抗原( CEA)、神经元特异性烯醇化酶(NSE)及细胞角质蛋白19片段(Cyfra21-1),并以病理检测结果为金标准,评价其在癌性胸腔积液鉴别诊断中的诊断效能。结果 SP70在诊断癌性胸腔积液时的灵敏度、特异度、准确度、阳性预测值、阴性预测值分别为70.0%、88.0%、79.0%、85.4%、74.6%。鳞癌、腺癌SP70抗原阳性检出率分别为76.5%和83.0%,均高于小细胞癌的26.3%,差异有统计学意义(χ2=12.628,19.549,均P<0.05)。结论与传统的肿瘤标志物相比,SP70抗原在癌性胸腔积液鉴别诊断中具有更高的诊断效能,检测方法简单、无创,有较高的临床应用价值。%Objective To investigate the clinical application value of SP70 antigen in differential diag-nosis of malignant pleural effusion.Methods A total of 200 patients with pleural effusion admitted in Dazhou Army Hospital from Jan.2013 to Jan.2014 were selected as research objects,containing 100 cases of malignant pleural effusion and 100 cases of non-malignant pleural effusion.Levels of SP70 antigen and tumor markers carcino-embryonic antigen(CEA),neuron-specific enolase(NSE),cytokeratin19 fragment(Cyfra21-1) in pleural effusion were detected in all the patients.Taking pathologic detection results as the golden stand-ard,the value of SP70 was assessed in the diagnosis of malignant pleural effusion.Results The sensitivity, specificity,accuracy,positive predictive value,and negative predictive value of SP70 antigen in the diagnosis of malignant pleural effusion was 70.0%,88.0%,79.0%,85.4%,74.6%.Positive rates of squamous cell

  2. Pre-treatment levels of C-reactive protein and squamous cell carcinoma antigen for predicting the aggressiveness of pharyngolaryngeal carcinoma.

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    Hsuan-Ho Chen

    Full Text Available The levels of squamous cell carcinoma antigen (SCC-Ag and C-reactive protein (CRP can be used to predict tumor invasion, lymph node metastasis, staging and survival in patients with oral cavity cancer. The present study analyzed the relationship between pre-treatment levels of SCC-Ag and CRP in relation to clinicopathological factors in patients with pharyngolaryngeal cancer (PLC and determined whether elevated levels of CRP and SCC-Ag were associated with tumor metabolic activity via [18F] fluorodeoxyglucose positron emission tomography (FDG-PET. We retrospectively recruited one hundred and six PLC patients between June 2008 and December 2011. All patients received computed tomography (CT/magnetic resonance imaging (MRI and FDG-PET staging analyses, and the serum levels of SCC-Ag and CRP in these patients were measured prior to treatment. A SCC-Ag level ≥2.0 ng/ml and a CRP level ≥5.0 mg/L were significantly associated with clinical stage (P<0.001, clinical tumor status (P<0.001, and clinical nodal status (P<0.001. The elevation of both SCC-Ag and CRP levels was correlated with the standardized uptake value (SUV max of the tumor (≥8.6 mg/L and lymph nodes (≥5.7 ng/ml (P = 0.019. The present study demonstrated that the presence of high levels of both pre-treatment SCC-Ag and CRP acts as a predictor of clinical stage, clinical tumor status, and clinical nodal status in patients with PLC. Moreover, elevated levels of SCC-Ag and CRP were associated with a high metabolic rate as well as the proliferative activity measured according to the SUVmax of the tumor and lymph nodes. Therefore, elevated levels of these two factors have the potential to serve as biomarkers for the prediction of tumor aggressiveness in cases of PLC.

  3. Therapeutic effect on patients with lung cancer and malignant pleural effusion by Transthoracoscopic resection with hyperthermic perfusion%胸腔镜下肿瘤切除并热灌注对肺癌恶性胸水的疗效分析

    Institute of Scientific and Technical Information of China (English)

    周华平

    2014-01-01

    目的:从肺癌相关的肿瘤标志物角度分析胸腔镜下胸膜肿瘤切除并胸膜热灌注术对肺癌伴恶性胸水患者的治疗效果。方法肺癌伴随恶性胸水患者41例为研究组,进行胸腔镜下胸膜活检及肿瘤切除并热灌注;将同期进行单独胸腔化疗的患者34例作为对照组。观察两组治疗前后血清和胸水肺癌标记物含量变化,并比较患者生存率。结果与对照组比较,治疗后研究组胸水 CEA、Cyfra21-1、CA -125及血 Cyfra21-1、CA -125明显下降( P <0.05)。与治疗前比较,治疗后研究组胸水及血液CA-125、Cyfra21-1、CEA降低( P <0.05);研究组患者1年生存率75.6%,2年生存率43.9%,明显优于对照组( P <0.05)。结论从肺癌相关的肿瘤标志物角度提示胸腔镜下胸膜肿瘤切除并热灌注术对于肺癌伴恶性胸水患者治疗效果好。%Objective To analysis the effect of transthoracoscopic resection with hyperthermic perfusion on lung cancer patients with malignant pleural effusion by detection of tumor markers related lung cancer ,Methods Re-search group included 41 patients with lung cancer with malignant hydrothorax ,using transthoracoscopic resection and hyperthermic perfusion .Control group included 34 cases ,who using the chest cavity chemotherapy alone in the corresponding time period .Observe the variance of lung cancer markers in serum and pleural effusion of two groups before and after treatment ,and compare the survival rate of two groups .Results Compared with the control group ,the CEA ,Cyfra21 -1 ,CA -125 in pleural fluid and Cyfra21 -1 ,CA -125 in blood serum of reserch group decreased ( P <0 .05) ,and the serum CA-125 ,Cyfra21-1 ,CEA of research group decreased signifi-cantly after the treatment ( P <0 .05 ) .The one year survival rate of patients in research group was 75 .6% ,two years survival rate 43 .9% ,significantly higher than the control group ( P <0 .05

  4. Bullying in Brazilian schools: results from the National School-based Health Survey (PeNSE, 2009 Bullying nas escolas brasileiras: resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE, 2009

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    Deborah Carvalho Malta

    2010-10-01

    Full Text Available The aim of this study is to identify and describe the occurrence of bullying among students in the 9th year (8th grade from public and private schools from 26 Brazilian state capitals and the Federal District. It is a cross-sectional study involving 60,973 students and 1,453 public and private schools. Data analysis indicates that 5.4% (IC95%: 5.1%-5.7% of students reported having suffered bullying almost always or always in the last 30 days, 25.4% (IC95%: 24.8%-26.0% were rarely or sometimes the victim of bullying and 69.2% (IC95%: 68.5%-69.8% of students felt no humiliation or provocation at school. The capital with higher frequency of bullying was Belo Horizonte (6.9%; IC95%: 5,9%-7,9%, Minas Gerais, and the lowest was Palmas (3.5%; IC95%: 2.6%-4.5%, Tocantins. Boys reported more bullying (6,0%; IC95%: 5.5%-6.5% compared with girls (4,8%; IC95%: 4.4%-5.3%. There was no difference between public schools 5.5% (IC95%: 5.1%-5.8% and private (5.2% (IC95%: 4.6%-5.8%, except in Aracaju, Sergipe, that show more bullying in private schools. The findings indicate an urgent need for intersectoral action from educational policies and practices that enforce the reduction and prevention of the occurrence of bullying in schools in Brazil.O objetivo deste estudo é identificar e descrever a ocorrência do bullying, episódios de humilhação ou provocação perpetrados pelos colegas da escola, entre estudantes do 9º ano do ensino fundamental de escolas públicas e privadas das 26 capitais dos estados brasileiros e do Distrito Federal. Trata-se de estudo transversal feito com 60.973 escolares de 1.453 escolas públicas e privadas. A análise dos dados aponta que 5,4% (IC95%: 5,1%-5,7% dos estudantes relataram ter sofrido bullying quase sempre ou sempre nos últimos 30 dias; 25,4% (IC95%: 24,8%-26,0% foram raramente ou às vezes vítimas de bullying e 69,2% (IC95%: 68,5%-69,8% não sentiram nenhuma humilhação ou provocação (bullying. A capital com maior frequência de foi Belo Horizonte-MG (6,9%; IC95%: 5,9%-7,9 e a menor foi Palmas-TO (3,5%; IC95%: 2,6%-4,5%. Meninos relatam mais bullying (6,0%; IC95%: 5,5%-6,5% do que meninas (4,8%; IC95%: 4,4%-5,3%. Não houve diferença entre escolas públicas (5,5%; IC95%: 5,1%-5,8% e privadas (5,2%; IC95%: 4,6%-5,8%, exceto em Aracaju-SE, onde foi registrada maior ocorrência de bullying em escolas privadas. Os dados mostram urgente necessidade de ações intersetoriais a partir de políticas e práticas educativas que efetivem redução e prevenção da ocorrência do bullying nas escolas.

  5. 五项肿瘤标志物联合检测对食管癌诊治的临床评价%Clinical Evaluation of Combined Detection of Five Tumor Markers in the Diagnosis and Therapy of Esophageal Cancer

    Institute of Scientific and Technical Information of China (English)

    张爱敏; 焦晓青; 张鹏

    2008-01-01

    探讨肿瘤标志物癌胚抗原(CEA)、糖类抗原19-9(CA19-9)、CA724、细胞角蛋白19片断(CYFRA21-1)和鳞状上皮细胞癌抗原(SCC)联合检测对食管癌诊断、治疗及预后判断的临床价值.用电化学发光免疫分析法(ECLIA)和微粒子酶联免疫测定法(MEIA)检测102例食管癌患者术前和90例术后血清中CEA、CA19-9、CA724、CYFRA21-1和SCC含量.102例食管癌患者血清中5项肿瘤标志物含量均明显高于对照组,随病程增加,阳性率增高.检测90例食管癌患者,术前阳性指标与术后比较,有显著性差异(P<0.01).五项肿瘤标志物联合检测阳性率为77.5%,高于单项指标检测阳性率,有显著性差异(P<0.01).血清CEA、CA19-9、CA724、CYFRA21-1和SCC水平动态联合监测可用于食管癌辅助诊断、疗效观察、以及对病期及预后的判断.

  6. Clinical diagnostic value of Molybdenum Target X-ray combined with four Serum tumor markers in the detection of Mastocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Ding-Hua Xu; Chun-Xian Cai; Guang-Xue Huang

    2015-01-01

    Objective: To explore the clinical diagnostic value of Molybdenum Target X-ray combined with serum CA15-3, CA125, CEA and CYFRA21-1 tumor markers in the detection of Mastocarcinoma. Method:90 cases confirmed by surgery pathology diagnosis according to the diagnosis of breast nodules disease patients were divided into benign tumor group (n=32) and breast cancer group (n=58), all patients were with preoperative line of molybdenum target X-ray radiography examination; Another group chose healthy women of our hospital for check-up in 56 cases as the control group, electrochemiluminescence immunoassay detection of three groups of participants were four kinds of serum tumor markers level, evaluation of single and combined testing the sensitivity of the diagnosis of breast cancer, specific degree, positive predictive value and negative predictive value. Results: CA15-3, CA125, CEA and CYFRA21-1 in breast cancer group were significantly higher than that of the control group and benign tumor group, the difference was statistically significant (all P0.05); Molybdenum target X-ray slice of the sensitivity of the diagnosis of breast cancer was higher, but the specific degree was low, and lower the sensitivity of the serum tumor markers in the diagnosis of breast cancer, the specific degree was higher, the molybdenum target X-ray slice joint the sensitivity of the four tumor markers in the diagnosis of breast cancer was 89.66%, 78.13%. Conclusions: The combined Mastocarcinoma detection of Molybdenum Target X-ray with serum CA15-3, CA125, CEA and CYFRA21-1 tumor markers can improve the detective rate and plays an important role in Mastocarcinoma early detection.

  7. Predictive and prognostic value of circulating nucleosomes and serum biomarkers in patients with metastasized colorectal cancer undergoing Selective Internal Radiation Therapy

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    Fahmueller Yvonne

    2012-01-01

    Full Text Available Abstract Background Selective Internal Radiation Therapy (SIRT is a new and effective locoregional anticancer therapy for colorectal cancer patients with liver metastases. Markers for prediction of therapy response and prognosis are needed for the individual management of those patients undergoing SIRT. Methods Blood samples were prospectively and consecutively taken from 49 colorectal cancer patients with extensive hepatic metastases before, three, six, 24 and 48 h after SIRT to analyze the concentrations of nucleosomes and further laboratory parameters, and to compare them with the response to therapy regularly determined 3 months after therapy and with overall survival. Results Circulating nucleosomes, cytokeratin-19 fragments (CYFRA 21-1, carcinoembryonic antigen (CEA, C-reactive protein (CRP and various liver markers increased already 24 h after SIRT. Pretherapeutical levels of CYFRA 21-1, CEA, cancer antigen 19-9 (CA 19-9, asparate-aminotransferase (AST and lactate dehydrogenase (LDH as well as 24 h values of nucleosomes were significantly higher in patients suffering from disease progression (N = 35 than in non-progressive patients (N = 14. Concerning overall survival, CEA, CA 19-9, CYFRA 21-1, CRP, LDH, AST, choline esterase (CHE, gamma-glutamyl-transferase, alkaline phosphatase, and amylase (all 0 h, 24 h and nucleosomes (24 h were found to be prognostic relevant markers in univariate analyses. In multivariate Cox-Regression analysis, the best prognostic model was obtained for the combination of CRP and AST. When 24 h values were additionally included, nucleosomes (24 h further improved the existing model. Conclusion Panels of biochemical markers are helpful to stratify pretherapeutically colorectal cancer patients for SIR-therapy and to early estimate the response to SIR-therapy.

  8. Increased GFAP and S100beta but not NSE serum levels after subarachnoid haemorrhage are associated with clinical severity.

    NARCIS (Netherlands)

    Vos, P.E.; Gils, M. van; Beems, T.; Zimmerman, C.; Verbeek, M.M.

    2006-01-01

    Assessment of initial disease severity after subarachnoid haemorrhage (SAH) remains difficult. The objective of the study is to identify biochemical markers of brain damage in peripheral blood after SAH. Hospital admission S100beta, glial fibrillary acidic protein (GFAP) and neuron-specific enolase

  9. Consumo e comportamento alimentar entre adolescentes brasileiros: Pesquisa Nacional de Saúde do Escolar (PeNSE), 2009

    OpenAIRE

    LEVY, Renata Bertazzi; Castro,Inês Rugani Ribeiro de; CARDOSO, Letícia Oliveira; Tavares,Letícia Ferreira; Sardinha,Luciana Monteiro Vasconcelos; Gomes,Fabio da Silva; COSTA, André Wallace Nery da

    2010-01-01

    O objetivo deste artigo é descrever características de consumo e comportamento alimentar de adolescentes brasileiros e sua associação com fatores sociodemográficos. Estudou-se, em 2009, amostra representativa de alunos do 9º ano do ensino fundamental de escolas públicas e privadas das 26 capitais brasileiras e do Distrito Federal. Utilizou-se questionário autoaplicável sobre atributos sociodemográficos, consumo e comportamento alimentar, entre outros. Estimativas dos indicadores construídos f...

  10. [Bullying in Brazilian schools: results from the National School-based Health Survey (PeNSE), 2009].

    Science.gov (United States)

    Malta, Deborah Carvalho; Silva, Marta Angélica Iossi; Mello, Flavia Carvalho Malta de; Monteiro, Rosane Aparecida; Sardinha, Luciana Monteiro Vasconcelos; Crespo, Claudio; Carvalho, Mércia Gomes Oliveira de; Silva, Marta Maria Alves da; Porto, Denise Lopes

    2010-10-01

    The aim of this study is to identify and describe the occurrence of bullying among students in the 9th year (8th grade) from public and private schools from 26 Brazilian state capitals and the Federal District. It is a cross-sectional study involving 60,973 students and 1,453 public and private schools. Data analysis indicates that 5.4% (IC95%: 5.1%-5.7%) of students reported having suffered bullying almost always or always in the last 30 days, 25.4% (IC95%: 24.8%-26.0%) were rarely or sometimes the victim of bullying and 69.2% (IC95%: 68.5%-69.8%) of students felt no humiliation or provocation at school. The capital with higher frequency of bullying was Belo Horizonte (6.9%; IC95%: 5,9%-7,9%), Minas Gerais, and the lowest was Palmas (3.5%; IC95%: 2.6%-4.5%), Tocantins. Boys reported more bullying (6,0%; IC95%: 5.5%-6.5%) compared with girls (4,8%; IC95%: 4.4%-5.3%). There was no difference between public schools 5.5% (IC95%: 5.1%-5.8%) and private (5.2%) (IC95%: 4.6%-5.8%), except in Aracaju, Sergipe, that show more bullying in private schools. The findings indicate an urgent need for intersectoral action from educational policies and practices that enforce the reduction and prevention of the occurrence of bullying in schools in Brazil.

  11. Bullying in Brazilian school children: analysis of the National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To describe the victimization and bullying practice in Brazilian school children, according to data from the National Adolescent School-based Health Survey and to compare the surveys from 2009 and 2012. METHODS: This is a cross-sectional study with univariate and multivariate analyzes of the following variables: to have been treated badly by colleagues, to have been bullied and to have bullied other children. The following independent variables were analyzed: age, sex, race/color, type of school, maternal education. Prevalence rates were compared between the editions of 2009 and 2012 of the survey. RESULTS: Of all the adolescents analyzed, 27.5% have not been treated well by peers at school, with greater frequency among boys (OR = 1.50, at the age of 15 years (OR = 1.29 and 16 (OR = 1.41, public school students (OR = 2.08, black (OR = 1.18 and whose mothers had less education; 7.2% reported having been bullied, with a greater chance in younger students (13 years old, male (OR = 1.26, black (OR = 1.15 and indigenous (OR = 1.16 and whose mothers had less education; 20.8% reported to have bullied other children, with a greater chance for older students, at the age of 14 (OR = 1.08 and 15 years (OR = 1.18, male (OR = 1.87, black (OR = 1.14 and yellow (OR = 1.15, children of mothers with higher education, private school students. There was an increase of bullying in the Brazilian capitals, from 5.4 to 6.8%, between 2009 and 2012. DISCUSSION: The occurrence of bullying reveals that the Brazilian school context is also becoming a space of reproduction of violence, in which it is crucial to act intersectorally and to articulate social protection networks, aiming to face this issue.

  12. [Utility of NSE, ProGRP and LDH in Diagnosis and Treatment
in Patients with Small Cell Lung Cancer].

    Science.gov (United States)

    Peng, Yan; Wang, Yan; Li, Junling; Hao, Xuezhi; Hu, Xingsheng

    2016-09-20

    背景与目的 小细胞肺癌(small cell lung cancer, SCLC)是一种生长迅速、具有神经内分泌特性的肿瘤。血清神经元特异性烯醇化酶(neuron specific enolase, NSE)、胃泌素释放肽前体(pro-gastrin-releasing peptide, ProGRP)和乳酸脱氢酶(lactic dehydrogenase, LDH)已在SCLC的诊断和治疗中起到一定的辅助作用,本研究旨在通过治疗前后SCLC患者NSE、ProGRP和LDH的变化探讨标志物在肿瘤分期、疗效评价及预测复发方面的价值。方法 纳入中国医学科学院肿瘤医院的SCLC初治病例,回顾性分析其临床数据,包括临床特征、治疗前及2周期化疗后的血清NSE、ProGRP及LDH,疗效及无进展生存期。结果 治疗前,广泛期(extensive disease, ED)患者NSE、ProGRP及LDH均高于局限期(limited disease, LD)(P4个及治疗后ProGRP下降明显的患者较化疗周期数≤4个及ProGRP下降不明显的患者复发风险低;而远处转移数目≤2个、疗前LDH正常及治疗后ProGRP的明显下降,提示ED患者的近期复发风险低。此外,肿瘤复发类型(敏感复发、耐药复发、难治复发)与化疗后ProGRP下降程度呈负相关(P=0.044)。多因素分析结果提示治疗周期数是LD组SCLC近期复发的独立影响因素,远处转移数目及治疗后ProGRP的下降程度是ED组SCLC近期复发的独立影响因素。结论 血清肿瘤标志物升高的程度与肿瘤负荷相关,ProGRP在治疗后的下降程度可能预测疗效及复发风险。.

  13. Relevance of circulating nucleosomes and oncological biomarkers for predicting response to transarterial chemoembolization therapy in liver cancer patients

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    Durner Jürgen

    2011-05-01

    Full Text Available Abstract Background Transarterial chemoembolization (TACE therapy is an effective locoregional treatment in hepatocellular cancer (HCC patients. For early modification of therapy, markers predicting therapy response are urgently required. Methods Here, sera of 50 prospectively and consecutively included HCC patients undergoing 71 TACE therapies were taken before and 3 h, 6 h and 24 h after TACE application to analyze concentrations of circulating nucleosomes, cytokeratin-19 fragments (CYFRA 21-1, alpha fetoprotein (AFP, C-reactive protein (CRP and several liver biomarkers, and to compare these with radiological response to therapy. Results While nucleosomes, CYFRA 21-1, CRP and some liver biomarkers increased already 24 h after TACE, percental changes of nucleosome concentrations before and 24 h after TACE and pre- and posttherapeutic values of AFP, gamma-glutamyl-transferase (GGT and alkaline phosphatase (AP significantly indicated the later therapy response (39 progression versus 32 no progression. In multivariate analysis, nucleosomes (24 h, AP (24 h and TACE number were independent predictive markers. The risk score of this combination model achieved an AUC of 81.8% in receiver operating characteristic (ROC curves and a sensitivity for prediction of non-response to therapy of 41% at 97% specificity, and of 72% at 78% specificity. Conclusion Circulating nucleosomes and liver markers are valuable tools for early estimation of the efficacy of TACE therapy in HCC patients.

  14. The relevance of serum carcinoembryonic antigen as an indicator of brain metastasis detection in advanced non-small cell lung cancer.

    Science.gov (United States)

    Lee, Dong-Soo; Kim, Yeon-Sil; Jung, So-Lyoung; Lee, Kyo-Young; Kang, Jin-Hyoung; Park, Sarah; Kim, Young-Kyoon; Yoo, Ie-Ryung; Choi, Byung-Ock; Jang, Hong-Seok; Yoon, Sei-Chul

    2012-08-01

    Although many biomarkers have emerged in non-small cell lung cancer (NSCLC), the predictive value of site-specific spread is not fully defined. We designed this study to determine if there is an association between serum biomarkers and brain metastasis in advanced NSCLC. We evaluated 227 eligible advanced NSCLC patients between May 2005 and March 2010. Patients who had been newly diagnosed with stage IV NSCLC but had not received treatment previously, and had available information on at least one of the following pretreatment serum biomarkers were enrolled: carcinoembryonic antigen (CEA), cytokeratin 19 fragments (CYFRA 21-1), cancer antigen 125 (CA 125), cancer antigen 19-9, and squamous cancer cell antigen. Whole body imaging studies and magnetic resonance imaging of the brain were reviewed, and the total number of metastatic regions was scored. Brain metastasis was detected in 66 (29.1%) patients. Although serum CEA, CYFRA 21-1, and CA 125 levels were significantly different between low total metastatic score group (score 1-3) and high total metastatic score group (score 4-7), only CEA level was significantly different between patients with brain metastasis and those without brain metastasis (p present study demonstrated that the pretreatment serum CEA level was significantly correlated with brain metastasis in advanced NSCLC. These findings suggested the possible role of CEA in the pathogenesis of brain invasion. More vigilant surveillance would be warranted in the high-risk group of patients with high serum CEA level and multiple synchronous metastasis.

  15. Diagnostic Accuracy of Urinary Cytokeratin 19 Fragment for Endometriosis

    Science.gov (United States)

    Savaris, R. F.; Ali, S.; Brophy, S.; Tomazic-Allen, S.; Chwalisz, K.

    2015-01-01

    Endometriosis affects up to 10% of women of reproductive age and 176 million women worldwide. The prevalence in women with infertility is between 30% and 50% but may be higher in women with pelvic pain, interstitial cystitis, or irritable bowel syndrome. Cytokeratin 19 has been suggested as a potential biomarker in urine for the diagnosis of this condition. The objective of this study was to prospectively determine the accuracy and the performance of a urinary cytokeratin 19 (uCYFRA 21-1) test for diagnosing endometriosis. Ninety-eight consecutive women who underwent laparoscopy had a urinary sample obtained before surgery and were included in the study. Endometriosis was diagnosed by laparoscopy and pathology in 64.3% (63 of 98 women). The estimates and 95% confidence intervals for sensitivity, specificity, positive and negative predictive values, and likelihood ratios were 11.1% (4.5%-21.5%), 94.3% (80.8%-99.3%), 77.7% (39.9-97.1), 37% (27-47.9), 1.94 (0.43-8.86), and 0.94 (0.84-1.06), respectively. Despite the high specificity, the uCYFRA 21-1 test has limited value for clinical practice to discriminate between women with and without endometriosis. PMID:25296695

  16. Trend of the risk and protective factors of chronic diseases in adolescents, National Adolescent School-based Health Survey (PeNSE 2009 e 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To compare the prevalence of major risk and protection factors for chronic non-communicable diseases in school-aged children in Brazilian capitals surveyed in the National Adolescent School-based Health Survey in its two editions, 2009 and 2012. METHODS: The frequencies, with Confidence Interval of 95%, of the following demographic variables were compared: food intake, body image, physical activity, smoking, alcohol and other drugs. Prevalence was compared in the two editions of the survey. RESULTS: The proportion of students who attend two physical education classes a week was maintained at 49% between 2009 and 2012, increasing in public schools from 50.6% (95%CI 49.8 - 51.4 to 52.5% (95%CI 49.2 - 55.7, and decreasing in private schools. There was no change in the proportion of students who watch two hours or more of television daily, about 80%. As for body image, there was no change between the two editions, and about 60% considered themselves being of normal weight. There was a reduction in the percentage of adolescents who experienced cigarettes, from 24.2% (95%CI 23.6 - 24.8 to 22.3% (95%CI 21.4 - 23.2, and the prevalence of smoking was maintained at about 6% (there was no statistical difference between 2009 and 2012. The consumption of beans, fruits, sweets and soft drinks also decreased. Frequency of drug experimentation was of 8.7% (95%CI 8.3 - 9.1 in 2009, and 9.6% (95%CI 9.0 - 10.3 in 2012, with no difference between confidence intervals, and the frequency of alcohol experimentation was maintained at about 70%; the percentage of use in the past 30 days was also maintained at around 27%. CONCLUSION: In the Brazilian capitals, the vast majority of prevalence of risk factors were kept stable in the two editions of the National Survey of School. These data generate evidence to guide the implementation of public policies to minimize the exposure of adolescents to risk factors.

  17. Psychoactive substance use, family context and mental health among Brazilian adolescents, National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To evaluate the association between the consumption of psychoactive substances (tobacco, alcohol and illicit drugs and demographic variables, mental health and family context among school-aged children. METHODS: The National Adolescent School-based Health Survey was held with a national sample of 109,104 students. Data regarding demographic variables, family background and mental health were collected. Logistic regression was used to evaluate the associations of interest. RESULTS: Multivariate analyses showed that alcohol consumption was higher among girls, drug experimentation was more frequent among boys and that there was no difference between sexes for smoking. Being younger and mulatto were negatively associated with the use of tobacco, alcohol and illicit drugs. Also negatively associated with such risk behaviors were characteristics of the family context represented by: living with parents, having meals together and parental supervision (when parents know what the child does in their free time. Moreover, characteristics of mental health such as loneliness and insomnia were positively associated with use of tobacco, alcohol and illicit drugs. Not having friends was positively associated with use of tobacco and illicit drugs and negatively associated with alcohol use. CONCLUSIONS: The study shows the protective effect of family supervision in the use of tobacco, alcohol and drugs and, on the contrary, the increasing use of substances according to aspects of mental health, such as loneliness, insomnia and the fact of not having friends. The study's findings may support actions from health and education professionals, as well as from the government and families in order to prevent the use of these substances by adolescents.

  18. Bullying and associated factors among Brazilian adolescents: analysis of the National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To estimate the prevalence of bullying from the victim's perspective in Brazilian school children and to analyze its association with individual and family context variables. METHODS: An analysis of the data on 109,104 adolescents, obtained by the National Adolescent School-based Health Survey, held in schools in 2012, was carried out. An association model between bullying and explanatory variables was tested in different contexts: sociodemographic, risk behaviors, mental health and family context. Univariate and multivariate analyzes were performed, calculating the Odds Ratio and confidence intervals. RESULTS: The prevalence of bullying found in this study was of 7.2% (95%CI 6.6 - 7.8. A higher chance of bullying was found among male students (OR = 1.58; 95%CI 1.51 - 1.66, with an inverse relation between age and bullying, with the magnitude of risk among adolescents younger than 13 years of age being higher when compared to those with 16 years of age or more. Of individual risk behaviors, only being a smoker remained in the final model (OR = 1.11; 95%CI 1.01 - 1.23. Mental health variables associated with bullying were: feeling lonely (OR = 2.66; 95%CI 2.52 - 2.81, insomnia (OR = 1.92; 95%CI 1.80 - 2.05, not having friends (OR = 1.71; 95%CI 1.54 - 1.89, and, in the family context, those who skip class without telling their parents (OR = 1.13; 95%CI 1,07 - 1,19 and those who suffer physical abuse by family members (OR = 2.03; 95%CI 1.91 - 2.146. CONCLUSION: Bullying was associated to male students, younger, of black color, smokers, with mental health vulnerabilities and victims of domestic violence. This suggests the need for a holistic approach from education and health professionals, parents and the community in seeking measures for the prevention of bullying.

  19. Trend of the risk and protective factors of chronic diseases in adolescents, National Adolescent School-based Health Survey (PeNSE 2009 e 2012).

    Science.gov (United States)

    Malta, Deborah Carvalho; de Andreazzi, Marco Antonio Ratzsch; Oliveira-Campos, Maryane; Andrade, Silvania Suely Caribé de Araújo; de Sá, Naíza Nayla Bandeira; de Moura, Lenildo; Dias, Antonio José Ribeiro; Crespo, Claudio Dutra; da Silva Júnior, Jarbas Barbosa

    2014-01-01

    To compare the prevalence of major risk and protection factors for chronic non-communicable diseases in school-aged children in Brazilian capitals surveyed in the National Adolescent School-based Health Survey in its two editions, 2009 and 2012. The frequencies, with Confidence Interval of 95%, of the following demographic variables were compared: food intake, body image, physical activity, smoking, alcohol and other drugs. Prevalence was compared in the two editions of the survey. The proportion of students who attend two physical education classes a week was maintained at 49% between 2009 and 2012, increasing in public schools from 50.6% (95%CI 49.8 - 51.4) to 52.5% (95%CI 49.2 - 55.7), and decreasing in private schools. There was no change in the proportion of students who watch two hours or more of television daily, about 80%. As for body image, there was no change between the two editions, and about 60% considered themselves being of normal weight. There was a reduction in the percentage of adolescents who experienced cigarettes, from 24.2% (95%CI 23.6 - 24.8) to 22.3% (95%CI 21.4 - 23.2), and the prevalence of smoking was maintained at about 6% (there was no statistical difference between 2009 and 2012). The consumption of beans, fruits, sweets and soft drinks also decreased. Frequency of drug experimentation was of 8.7% (95%CI 8.3 - 9.1) in 2009, and 9.6% (95%CI 9.0 - 10.3) in 2012, with no difference between confidence intervals, and the frequency of alcohol experimentation was maintained at about 70%; the percentage of use in the past 30 days was also maintained at around 27%. In the Brazilian capitals, the vast majority of prevalence of risk factors were kept stable in the two editions of the National Survey of School. These data generate evidence to guide the implementation of public policies to minimize the exposure of adolescents to risk factors.

  20. Use of NSE/PS2m-transgenic mice in the study of the protective effect of exercise on Alzheimer's disease.

    Science.gov (United States)

    Cho, Jun Y; Hwang, Dae Y; Kang, Tae S; Shin, Dong H; Hwang, Jin H; Lim, Chae H; Lee, Su H; Lim, Hwa J; Min, Sae H; Seo, Su J; Song, Youn S; Nam, Ki T; Lee, Kyu S; Cho, Jung S; Kim, Yong K

    2003-11-01

    In its late stage, Alzheimer's disease results in progressive muscle weakness in the arms and legs. The aim of this study was to determine whether mice expressing the skeletal muscle-specific mutant PS2 gene (a model of Alzheimer's disease) are a useful experimental system to study the protective effect of exercise on A beta-42 reduction, improvement of behavioural function and changes in metabolic parameters. With this aim in mind, the transgenic mice were subjected to treadmill exercise for 3 months. The results showed that in transgenic mice, but not in normal mice, treadmill exercise resulted in a reduction of A beta-42 deposits and an improvement in behavioural function, thereby restoring normal concentrations of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and triglyceride. Thus, exercise may represent a practical therapeutic strategy for use with human patients with Alzheimer's disease.

  1. Situations of violence experienced by students in the state capitals and the Federal District: results from the National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To describe violent events experienced by school-aged adolescents in school, around the school and in the family context, and to compare the results of the National Adolescent School-based Health Survey of 2009 and 2012. METHODS: Indicators related to violence involving teenagers were analyzed. The prevalence rates and confidence intervals of 95% were calculated for events of interest according to gender and type of school (public or private and regions. RESULTS: Prevalence rates were: insecurity in the route between home-school (9.1%, insecurity in school (8.0%, physical assault in the last 12 months (18.2%, engaging in fights in the last 12 months (20.7%, fighting with a cold weapon (8.3%, fighting with firearms (6.9%, physical assault by a family member (11.6% and having been seriously injured in the last 12 months (10.3%. The situations of violence were more prevalent among male students from public schools. The comparison with the 2009 survey showed increased prevalence in all the variables studied. CONCLUSION: Teenagers are exposed to different forms of violence, and the data from the National Survey of School Health can support the planning of preventive actions.

  2. 18F-FDG PET/CT联合肿瘤标志物对肺癌的诊断价值及SUVmax的临床意义%Diagnostic value of 18F-FDG PET/CT imaging plus serum tumor marker assays for pulmonary lesions and clinical significance of SUVmax

    Institute of Scientific and Technical Information of China (English)

    张铁梅; 张连民; 刘洋; 张真发; 王长利

    2012-01-01

    Objective To evaluate the diagnostic value of 18F-FDG positron emission tomography/computed tomography (PET/CT) plus serum tumor marker assay in lung cancer and explore the correlation between standard uptake value (SUVmax) with clinicopathologic factors in lung cancer.Methods A total of 177 cases of lung cancer diagnosed by radiography or computed tomography (CT) were recruited.18F-FDG PET/CT imaging and detection of three lung cancer related serum markers (carcinoembryonic antigen,CYFRA21-1 and neuron specific enolase) were performed within one week in all cases.The sensitivity,specificity and accuracy of those approaches were calculated through comparing the results with pathologic examinations.Also the associations between SUVtnax and clinicopathologic features were analyzed.Results Among them,145 patients were detected to have lung cancer by pathologic diagnosis while the other 32 patients had benign lung diseases.The sensitivity,specificity,accuracy of 18F-FDG PET/CT imaging,serum tumor markers and their combination in assessing lung cancers were 89.7%,78.1%,87.6% ; 89.7%,78.1%,87.6% and 96.6%,56.3%,89.3% respectively.The combination of 18F-FDG PET/CT and serum tumor markers in lung lesions showed significantly higher sensitivity than serum tumor markers and 18F-FDG PET/CT alone(P =0.000,P =0.002).Its accuracy was also significantly higher than those of tumor markers (P < 0.05).Compared with 18 F-FDG PET/CT alone,the accuracy was higher in combination group.But the difference showed no statistical significance (P > 0.05).SUVmax was significantly associated with tumor staging,tumor size and pathologic type.Conclusion The combination of 18 F-FDG PET/CT and tumor markers may improve the positive diagnostic rate of lung cancer.And SUVmax can help to evaluate tumor staging and determine pathological types.%目的 评价18氟脱氧葡萄糖(18F-FDG) PET/CT显像联合肿瘤标志物测定对肺部肿块良恶性鉴别的诊断价值,并进一

  3. 应用FR靶向PCR法检测CTC在肺癌诊断中的临床价值:初步研究%Diagnostic Value of Folate Receptor-positive Circulating Tumor Cell in Lung Cancer:A Pilot Study

    Institute of Scientific and Technical Information of China (English)

    连欢欢; 丁志丹; 袁东风; 马杰; 秦建军

    2016-01-01

    背景与目的评价一种通过叶酸受体(folate receptor, FR)检测循环肿瘤细胞(circulating tumor cell, CTC)的方法用于肺癌临床诊断的实用性和可行性及进一步探究CTC在肺癌术后复发的预测价值。方法通过免疫磁珠负向富集方法从3 mL外周血中捕获循环肿瘤细胞,再用肿瘤特异性叶酸配体-寡核苷酸偶和物标记捕获的循环肿瘤细胞,洗去没有结合的偶和物后,洗脱下特异性结合的偶合物的寡核苷酸用于定量PCR扩增分析。结果97例肺癌患者的CTC水平高于肺部良性疾病患者(P<0.001)。本检测方法以8.7 Folate Units/3 mL为cutoff值,结果显示靶向PCR法对肺癌的检测灵敏度为82.5%,特异性为72.2%,特别是在I期肺癌灵敏度达到86.8%。与其他肿瘤标志物(NSE、CEA、CYFRA21-1)比较,CTC对肺癌及I期肺癌具有较高的诊断准确性(0.859;95%CI:0.779-0.939)和(0.912;95%CI:0.829-0.994)。5例肺癌患者术后2周内CTC水平高于cutoff值。结论叶酸受体阳性循环肿瘤细胞可以应用于肺癌的临床诊断,即使是对早期非小细胞肺癌(non-small cell lung cancer, NSCLC)的诊断。%Background and objective The aim of this study is to determine the effcacy and feasibility of a novel folate receptor (FR)-based circulating tumor cell (CTC) detection method in the diagnosis of lung cancer. CTCs were col-lected from 3 mL of blood based on negative enrichment by immunomagnetic beads and then labeled by a conjugate of a tumor-speciifc ligand folate and an oligonucleotide.Methods Atfer washing off redundant conjugates, the bound conjugates were removed and analyzed by quantitative polymerase chain reaction.Results The CTC levels of 97 patients with lung cancer were signiifcantly higher than that of the controls (18 patients with benign lung diseases;P<0.001). With a threshold of 8.7 Folate units, the method showed a sensitivity of 82.5% and a speciifcity of 72

  4. Preoperative serum squamous cell carcinoma antigen levels in clinical decision making for patients with early-stage cervical cancer

    NARCIS (Netherlands)

    Reesink-Peters, N; van der Velden, J; ten Hoor, KA; Boezen, HM; de Vries, EGE; Schilthuis, MS; Mourits, MJE; Nijman, HW; Aalders, JG; Hollema, H; Pras, E; Duk, JM; van der Zee, AGJ

    2005-01-01

    PURPOSE: To prevent morbidity associated with double modality treatment, early-stage cervical cancer patients should only be offered surgery when there is a low likelihood for adjuvant radiotherapy. We analyzed whether serum squamous cell carcinoma antigen (SCC-ag) analysis allows better preoperativ

  5. Comparative evaluation of serum C-reactive protein (CRP) levels in the different histological subtypes of esophageal cancer (squamous cell carcinoma and adenocarcinoma of esophagus).

    Science.gov (United States)

    Lukaszewicz-Zając, Marta; Mroczko, Barbara; Kozłowski, Mirosław; Nikliński, Jacek; Laudański, Jerzy; Siewko, Maria; Szmitkowski, Maciej

    2012-02-01

    Elevated C-reactive protein (CRP) levels have been found in patients with several malignancies. The aim of the present study was to analyze the diagnostic and prognostic values of CRP levels measurement in esophageal cancer (EC) patients in relation to its different histological subtypes (squamous cell carcinoma-ESCC and adenocarcinoma-AC of esophagus) and compared them with classic tumor markers-carcinoembryonic antigen (CEA) and squamous cell cancer antigen (SCC-Ag). The diagnostic sensitivity, specificity, and the areas under receiver operating characteristic curves (AUC) for all the proteins tested were defined. Serum CRP levels were statistically higher in EC, ESCC, and AC patients compared to healthy subjects and significantly increased in EC and ESCC patients with the presence of lymph node and distant metastases. The percentage of elevated CRP results in all the analyzed subgroups (EC, ESCC, and AC) was higher than CEA and SCC-Ag, similarly as AUC for CRP in comparison to SCC-Ag. Serum CRP level was a significant predictor of EC and ESCC patients' survival in univariate analysis. In conclusion, these results indicate that CRP can be used as an adjunct in evaluating the tumor markers-CEA and SCC-Ag and may improve the clinical diagnosis and follow-up of EC patients, especially for ESCC subgroup.

  6. Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients.

    Science.gov (United States)

    Ławicki, Sławomir; Będkowska, Grażyna E; Gacuta-Szumarska, Ewa; Knapp, Paweł; Szmitkowski, Maciej

    2012-07-04

    In this study, we compared plasma levels and the diagnostic utility of hematopoietic growth factors (HGFs) with SCC-Ag in cervical cancer patients in relation to control groups and cervical intraepithelial neoplasia (CIN) patients and healthy subjects. Pretreatment plasma levels of HGFs (SCF, GM-CSF, G-CSF and M-CSF) were determined by the use of immunoenzyme assay (ELISA), and SCC-Ag by chemiluminescent microparticle immunoassay (CMIA). Significantly different concentrations of GM-CSF, G-CSF and M-CSF were observed in the group of patients with cervical cancer and CIN compared to the healthy controls. Significant differences in plasma levels of GM-CSF and M-CSF between cervical cancer and benign lesions patients were also found. The HGFs and SCC-Ag diagnostic specificities received high values. The diagnostic sensitivity and the predictive value of a positive and negative test result were higher for M-CSF than for antigen SCC in the cancer group. The M-CSF area under the ROC curve (AUC) was the largest from hematopoietic cytokines and SCC-Ag. These results suggest the potential utility of M-CSF as a good candidate for a marker of cervical cancer as well as benign lesions of this organ (CIN).

  7. Assessment of Treg and Th17 contents in peripheral blood of patients with non-small cell lung cancer and analysis of their correlation with clinicopathologic characteristics

    Institute of Scientific and Technical Information of China (English)

    Jin Pu; Yuan-Yuan Zhou; Wen-Shu Ge; Hui-Lan Hong; Jing-Feng Zhang; Chong Bai

    2016-01-01

    Objective:To analyze Treg and Th17 contents in peripheral blood of patients with non-small cell lung cancer and their correlation with clinicopathologic characteristics.Methods:Peripheral blood and serum specimens were collected from patients with non-small cell lung cancer and healthy volunteers to detect Treg and Th17 contents in peripheral blood as well as CEA, TSGF, CYFRA21-1, PTX3 and HE4 contents in serum; tumor tissue and paracancer tissue were collected from lung cancer patients to detect mRNA expression of TRIM25, PKM2, Endoglin, Pin1, Stahmin and HMGA2.Results:Treg cell content and Treg/Th17 ratio in peripheral blood of patients with non-small cell lung cancer were significantly higher than those of healthy volunteers, and Th17 cell content was significantly lower than that of healthy volunteers; the higher the TNM stage and the lower the degree of differentiation, the higher the Treg cell content and Treg/Th17 ratio and the lower the Th17 cell content in peripheral blood; 1 week and 1 month after operation, Treg cell content and Treg/Th17 ratio in peripheral blood of patients with non-small cell lung cancer were lower than those before operation, and Th17 cell content was higher than that before treatment; serum CEA, TSGF, CYFRA21-1, PTX3 and HE4 contents of patients with non-small cell lung cancer were significantly higher than those of healthy volunteers and positively correlated with Treg/Th17 ratio; mRNA contents of TRIM25, PKM2, Endoglin, Pin1, Stahmin and HMGA2 in non-small cell lung cancer tissue were significantly higher than those in paracancer tissue and positively correlated with Treg/Th17 ratio.Conclusion:Increased Treg content and decreased Th17 content are related to the occurrence and development of non-small cell lung cancer.

  8. A study on the measuremet of DKK-1 in the serum of esophageal cancer patients%食管鳞癌患者血清DKK-1的检测及其临床意义

    Institute of Scientific and Technical Information of China (English)

    李波波; 李道堂; 赵东波; 王兴武

    2009-01-01

    目的 检测食管癌患者血清中DKK-1(dickkopf-1)的表达水平,探讨其在食管癌辅助诊断中的价值.方法 应用酶联免疫吸附法(ELISA)检测80例食管鳞癌患者和35例健康对照组血清中DKK-1的浓度,计算DKK-1诊断食管鳞癌的敏感性、特异性、阳性预测值和阴性预测值,并与细胞角蛋白19片段(CYFRA21-1)和癌胚抗原(CEA)的检测做比较,探讨其在食管癌辅助诊断中的价值.结果 ①食管鳞癌患者血清中DICK-1浓度为90.44±57.41 ng/ml,而正常对照组为11.29±9.45 ng/ml,两者差异有统计学意义(P0.05),而与肿瘤分期和淋巴结转移密切相关(P0.05). And the sensitivity of DKK-1 (66. 25%) was significantly higher than that of CEA, CYFRA21-1 (39. 44% ,25. 35%, P <0. 01). Conclusion DKK-1 played a very important role in the growth and metastasis of esophageal cancer and may be a new tumor marker or an important index in the diagno-sis of esophageal cancer.

  9. Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic biomarker and a therapeutic target for lung and esophageal carcinomas.

    Science.gov (United States)

    Ishikawa, Nobuhisa; Takano, Atsushi; Yasui, Wataru; Inai, Kouki; Nishimura, Hitoshi; Ito, Hiroyuki; Miyagi, Yohei; Nakayama, Haruhiko; Fujita, Masahiro; Hosokawa, Masao; Tsuchiya, Eiju; Kohno, Nobuoki; Nakamura, Yusuke; Daigo, Yataro

    2007-12-15

    Gene expression profile analyses of non-small cell lung carcinomas (NSCLC) and esophageal squamous cell carcinomas (ESCC) revealed that lymphocyte antigen 6 complex locus K (LY6K) was specifically expressed in testis and transactivated in a majority of NSCLCs and ESCCs. Immunohistochemical staining using 406 NSCLC and 265 ESCC specimens confirmed that LY6K overexpression was associated with poor prognosis for patients with NSCLC (P = 0.0003), as well as ESCC (P = 0.0278), and multivariate analysis confirmed its independent prognostic value for NSCLC (P = 0.0035). We established an ELISA to measure serum LY6K and found that the proportion of the serum LY6K-positive cases was 38 of 112 (33.9%) NSCLC and 26 of 81 (32.1%) ESCC, whereas only 3 of 74 (4.1%) healthy volunteers were falsely diagnosed. In most cases, there was no correlation between serum LY6K and conventional tumor markers of carcinoembryonic antigen (CEA) and cytokeratin 19-fragment (CYFRA 21-1) values. A combined ELISA for both LY6K and CEA classified 64.7% of lung adenocarcinoma patients as positive, and the use of both LY6K and CYFRA 21-1 increased sensitivity in the detection of lung squamous cell carcinomas and ESCCs up to 70.4% and 52.5%, respectively, whereas the false positive rate was 6.8% to 9.5%. In addition, knocked down of LY6K expression with small interfering RNAs resulted in growth suppression of the lung and esophageal cancer cells. Our data imply that a cancer-testis antigen, LY6K, should be useful as a new type of tumor biomarker and probably as a target for the development of new molecular therapies for cancer treatment.

  10. Nanostructured zirconia decorated reduced graphene oxide based efficient biosensing platform for non-invasive oral cancer detection.

    Science.gov (United States)

    Kumar, Suveen; Sharma, Jai Gopal; Maji, Sagar; Malhotra, Bansi Dhar

    2016-04-15

    We report results of the studies relating to fabrication of a non-invasive, label-free and an efficient biosensing platform for detection of the oral cancer biomarker (CYFRA-21-1). One step hydrothermal process was used for uniform decoration of nanostructured zirconia (average particle size 13 nm) on reduced graphene oxide (ZrO2-RGO) to avoid coagulation of the zirconia nanoparticles and to obtain enhanced electrochemical performance of ZrO2-RGO nanocomposite based biosensor. Further, ZrO2-RGO has been functionalized using 3-aminopropyl triethoxy saline (APTES) and electrophoretically deposited on the indium tin oxide coated glass substrate at a low DC potential.The APTES/ZrO2-RGO/ITO electrode exhibits improved heterogeneous electron transfer (more than two times) with respect to that of the APTES/ZrO2/ITO electrode indicating faster electron transfer kinetics. The -NH2 containing APTES/ZrO2-RGO/ITO platform is further biofunctionalized with anti-CYFRA-21-1. The structural and morphological investigations of the ZrO2-RGO based biosensing platform have been accomplished using X-ray diffraction (XRD), electrochemical, transmission electron microscopy (TEM), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) studies. This immunosensor exhibits a wider linear detection range (2-22 ng mL(-1)), excellent sensitivity (0.756 µA mL ng(-1)) and a remarkable lower detection limit of 0.122 ng mL(-1). The observed results have been validated via enzyme linked immunosorbent assay (ELISA).

  11. Prognostic impact of cytological fluid tumor markers in non-small cell lung cancer.

    Science.gov (United States)

    Cho, Arthur; Hur, Jin; Hong, Yoo Jin; Lee, Hye-Jeong; Kim, Young Jin; Hong, Sae Rom; Suh, Young Joo; Im, Dong Jin; Kim, Yun Jung; Lee, Jae Seok; Shim, Hyo Sup; Choi, Byoung Wook

    2016-03-01

    The serum tumor markers CYFRA 21-1, carcinoembryonic antigen (CEA), and squamous cell carcinoma antigen (SCCA) are useful in diagnosis and prognosis of non-small cell lung cancer (NSCLC). Cytologic tumor markers obtained during needle aspiration biopsies (NAB) of lung lesions are useful for NSCLC diagnosis. This study investigated the incremental prognostic value of cytologic tumor markers compared to serum tumor markers. This prospective study included 253 patients diagnosed with NSCLC by NAB with cytologic tumor marker analysis. Levels of cytologic CYFRA 21-1, CEA, SCCA, and their serum counterparts were followed up for survival analysis. Optimal cutoff values for each tumor marker were obtained for overall survival (OS) and progression-free survival (PFS) analyses. All patients were followed up for a median of 22.8 months. Using cutoff values of 0.44 ng/ml for C-SCCA, 2.0 ng/ml for S-SCCA, and 3.3 ng/ml for S-CYFRA, a multivariate analysis revealed that high S-SCCA (hazard ratio, HR, 1.84) and high C-SCCA (HR, 1.63) were independent predictive factors of OS. The 3-year overall survival rate was 55 vs. 80 % for high and low C-SCCA, respectively. Cytologic tumor marker level detection is easily obtainable and provides prognostic information for NSCLC. Cytologic tumor markers provide comparable prognostic information relative to serum tumor markers, with C-SCCA acting as a strong prognostic factor of overall survival and PFS.

  12. Evaluation of a CLEIA automated assay system for the detection of a panel of tumor markers.

    Science.gov (United States)

    Falzarano, Renato; Viggiani, Valentina; Michienzi, Simona; Longo, Flavia; Tudini, Silvestra; Frati, Luigi; Anastasi, Emanuela

    2013-10-01

    Tumor markers are commonly used to detect a relapse of disease in oncologic patients during follow-up. It is important to evaluate new assay systems for a better and more precise assessment, as a standardized method is currently lacking. The aim of this study was to assess the concordance between an automated chemiluminescent enzyme immunoassay system (LUMIPULSE® G1200) and our reference methods using seven tumor markers. Serum samples from 787 subjects representing a variety of diagnoses, including oncologic, were analyzed using LUMIPULSE® G1200 and our reference methods. Serum values were measured for the following analytes: prostate-specific antigen (PSA), alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), carbohydrate antigen 15-3 (CA15-3), carbohydrate antigen 19-9 (CA19-9), and cytokeratin 19 fragment (CYFRA 21-1). For the determination of CEA, AFP, and PSA, an automatic analyzer based on chemiluminescence was applied as reference method. To assess CYFRA 21-1, CA125, CA19-9, and CA15-3, an immunoradiometric manual system was employed. Method comparison by Passing-Bablok analysis resulted in slopes ranging from 0.9728 to 1.9089 and correlation coefficients from 0.9977 to 0.9335. The precision of each assay was assessed by testing six serum samples. Each sample was analyzed for all tumor biomarkers in duplicate and in three different runs. The coefficients of variation were less than 6.3 and 6.2 % for within-run and between-run variation, respectively. Our data suggest an overall good interassay agreement for all markers. The comparison with our reference methods showed good precision and reliability, highlighting its usefulness in clinical laboratory's routine.

  13. Detection of colorectal neoplasia: Combination of eight blood-based, cancer-associated protein biomarkers.

    Science.gov (United States)

    Wilhelmsen, Michael; Christensen, Ib J; Rasmussen, Louise; Jørgensen, Lars N; Madsen, Mogens R; Vilandt, Jesper; Hillig, Thore; Klaerke, Michael; Nielsen, Knud T; Laurberg, Søren; Brünner, Nils; Gawel, Susan; Yang, Xiaoqing; Davis, Gerard; Heijboer, Annemieke; Martens, Frans; Nielsen, Hans J

    2017-03-15

    Serological biomarkers may be an option for early detection of colorectal cancer (CRC). The present study assessed eight cancer-associated protein biomarkers in plasma from subjects undergoing first time ever colonoscopy due to symptoms attributable to colorectal neoplasia. Plasma AFP, CA19-9, CEA, hs-CRP, CyFra21-1, Ferritin, Galectin-3 and TIMP-1 were determined in EDTA-plasma using the Abbott ARCHITECT® automated immunoassay platform. Primary endpoints were detection of (i) CRC and high-risk adenoma and (ii) CRC. Logistic regression was performed. Final reduced models were constructed selecting the four biomarkers with the highest likelihood scores. Subjects (N = 4,698) were consecutively included during 2010-2012. Colonoscopy detected 512 CRC patients, 319 colonic cancer and 193 rectal cancer. Extra colonic malignancies were detected in 177 patients, 689 had adenomas of which 399 were high-risk, 1,342 had nonneoplastic bowell disease and 1,978 subjects had 'clean' colorectum. Univariable analysis demonstrated that all biomarkers were statistically significant. Multivariate logistic regression demonstrated that the blood-based biomarkers in combination significantly predicted the endpoints. The reduced model resulted in the selection of CEA, hs-CRP, CyFra21-1 and Ferritin for the two endpoints; AUCs were 0.76 and 0.84, respectively. The postive predictive value at 90% sensitivity was 25% for endpoint 1 and the negative predictive value was 93%. For endpoint 2, the postive predictive value was 18% and the negative predictive value was 97%. Combinations of serological protein biomarkers provided a significant identification of subjects with high risk of the presence of colorectal neoplasia. The present set of biomarkers could become important adjunct in early detection of CRC. © 2016 UICC.

  14. Estado nutricional dos adolescentes e sua relação com variáveis sociodemográficas: Pesquisa Nacional de Saúde do Escolar (PeNSE, 2009 Nutritional status of adolescents and its relation with socio-demographics variables: National Adolescent School-based Health Survey (PeNSE, 2009

    Directory of Open Access Journals (Sweden)

    Cora Araújo

    2010-10-01

    Full Text Available Objetivou-se descrever o estado nutricional dos adolescentes da 9ª série de escolas públicas e privadas das capitais brasileiras e sua associação com variáveis sociodemográficas. Por meio de questionário autopreenchido, foram registradas informações sobre sexo, raça/cor, idade, escola pública/privada, macrorregião do país, escolaridade materna e posse de bens familiares. Peso e altura foram aferidos segundo procedimentos padronizados. A altura para idade e o Índice de Massa Corporal para idade foram avaliados segundo critérios da Organização Mundial da Saúde. Obtiveram-se medidas antropométricas de 58.971 adolescentes, sendo que 2,9% apresentaram déficit estatural e 2,9% magreza. O excesso de peso foi de 23,0% e a obesidade de 7,3%, sendo mais altos no Sul e no Sudeste do país. Déficits de altura e peso, excesso de peso e obesidade foram mais prevalentes nos meninos. Os déficits foram superiores nas escolas públicas, e o excesso de peso e a obesidade nas privadas. Adolescentes cujas mães eram de menor escolaridade ou de famílias mais pobres apresentaram maior déficit de altura; o inverso ocorreu com o excesso de peso e a obesidade. Prevalências de déficits, excesso de peso e obesidade ressaltam a necessidade de intervenções nutricionais direcionadas para esta fase da vida.The aim of this study was to describe the nutritional status of adolescents in the 9th year of public and private schools of the Brazilian capitals and its association with socio-demographic variables. By means of a self-completed questionnaire, information related to sex, race, age, public/private school, macro-region of the country, maternal education and family possessions were recorded. Measures of weight and height were measured according to standardized procedures. Height for age and body mass index for age was evaluated according to the World Health Organization reference. Anthropometric measurements of 58,971 adolescents were obtained, of which 2.9% were stunted and 2.9% underweight. There was 23.0% of overweight and 7.3% of obesity, which were higher in the South and Southeast regions. Deficits in height and weight, overweight and obesity were more prevalent in boys. The deficits were higher at public schools while overweight and obesity at private ones. Adolescents whose mothers were less educated or from poorer families had greater deficits in height and the reverse occurred with overweight and obesity. The findings about the deficits, overweight and obesity highlight the need for nutritional interventions designed to reach this stage of life.

  15. 鳞癌抗原在宫颈癌前病变及早期宫颈癌治疗中的价值%Value of squamous cell carcinoma antigen in cervical precancerous lesions and early cervical cancer therapy

    Institute of Scientific and Technical Information of China (English)

    王斌; 张月桃; 李琼珍

    2016-01-01

    目的:探讨鳞癌抗原在宫颈癌前病变及早期宫颈癌治疗中的价值。方法采用酶联接免疫吸附剂测定(ELISA)法初检,化学发光法复检测定宫颈上皮内瘤变(CINⅡ或 CINⅢ)、宫颈癌Ⅰ期与Ⅱa 期患者共134例不同临床分期宫颈鳞癌患者 SCC - Ag 水平和阳性率。术后将其分为两组(随访组和对照组),对照组只做常规宫颈病变筛查。随访两年观察比较两组患者术后两年内诊断出宫颈癌前病变的复发率。结果宫颈癌Ⅰ期和宫颈癌Ⅱa 期 SCC - Ag 水平、阳性率均明显高于宫颈上皮内瘤变(CINⅡ或 CINⅢ)组,差异具有统计学意义( P <0.05)。SCC - Ag 对术后检出宫颈肿瘤的复发率对比传统筛查方法,差异有显著性( P <0.05)。结论较高的血清 SCC 值可能意味着肿瘤恶性程度越高,血清 SCC - Ag 水平对于宫颈上皮内瘤变与宫颈癌早期的诊断、疗效判断、随访评估和复发后抢救性治疗措施均有重要的临床指导意义。%Objective To discuss the value of squamous cell carcinoma antigen in cervical precancerous lesions and early cervical cancer therapy. Methods Using ELISA for early detection,chemiluminescence review measurement of cervical intraepithelial neoplasia(CINⅡ or CINⅢ),patients with cervical cancer stage Ⅰ Ⅱa total 134 patients in different clinical stages of cervical squamous cell carcinoma SCC - Ag level and positive rate. They were divided into two groups after surgery,the control group only routine screening of cervical lesions,for a two - year fol-low - up. To observe and compare the recurrence rate of cervical lesions and early stage cervical cancer in two group of patients,more SCC - Ag value in the diagnosis of cervical cancer recurrence after surgery. Evaluating the value of SCC - Ag in the diagnosis of cervical cancer after surgical treatment of recurrence. Results The detection of postoperative recurrence rate of

  16. [Circulating biomarkers association in the follow-up of patients with oral cancer].

    Science.gov (United States)

    Colella, G; Cozzolino, A; Santagata, M; Vicidomini, A; Itro, A

    2001-05-01

    The goal of this study is to analyze the importance of circulating biomarkers association in the management of patients affected by oral cancer. In this study a survey is made of the international experience from 1980 to 1990 based on the presence of CEA, LASA, SCC Ag, TPA, ferritina, CA-50 and others in patients affected by oral cancer and the sensitivity and specificity of these circulating biomarkers association are assessed. In patients with active disease, the results obtained at the time of diagnosis of oral cancer are not satisfactory due to poor specificity of these circulating biomarkers association. The conclusions is drawn that the circulating biomarkers association (especially CEA, SCC Ag, LASA, ferritin, TPA and CA-50) appears to be useful in the prognosis and staging of oral cancer, while their presence is not significative for the diagnosis.

  17. Higher importance of interleukin 6 than classic tumor markers (carcinoembryonic antigen and squamous cell cancer antigen) in the diagnosis of esophageal cancer patients.

    Science.gov (United States)

    Łukaszewicz-Zając, M; Mroczko, B; Kozłowski, M; Nikliński, J; Laudański, J; Szmitkowski, M

    2012-04-01

    It has been suggested that interleukin 6 (IL-6) plays a potential role in the growth and progression of tumors, including esophageal cancer (EC). The aim of the study was to compare clinical significance of serum IL-6 with classic tumor markers - carcinoembryonic antigen (CEA) and squamous cell cancer antigen (SCC-Ag) - in EC patients in relation to its histological types - squamous cell carcinoma of esophagus (ESCC) and adenocarcinoma (AD) of esophagus. The study included 53 EC patients and 90 healthy subjects. Serum IL-6 and CEA levels were determined using immunoenzyme assays, while SCC-Ag - chemiluminescent assay. The diagnostic criteria and prognostic values for markers were defined. The levels of all proteins tested in EC, ESCC, and AD were higher than in healthy subjects. The percentage of elevated results was substantially higher for IL-6 (86%) than for CEA (30%) and SCC-Ag (24%) in EC, similarly as in ESCC (87%, 23%, and 33%) and AD (87%, 39%, and 13%, respectively) patients. Concentrations of IL-6 depended on distant metastases and patients' survival in EC and were significantly higher in ESCC patients with more advanced tumor stage and nodal metastases. The IL-6 area under receiver operating characteristic curve (0.92) was larger than for CEA (0.84) and SCC-Ag (0.62) in EC, likewise in ESCC (0.92, 0.87, 0.77) and AD (0.91, 0.79, 0.57, respectively). Our findings indicate better usefulness of IL-6 than classic tumor markers in the diagnosis of EC, especially in patients with ESCC.

  18. 颅脑损伤后血清S100 B、NSE检测的意义%Significance of Serum S100B and NSE Detection after Cerebral Injury

    Institute of Scientific and Technical Information of China (English)

    安翠红(综述); 张小平; 程爱国(审校)

    2015-01-01

    严重颅脑创伤及其后遗症可导致不同程度的神经系统功能障碍,特别是颅脑损伤后学习记忆功能障碍会对患者的生活质量产生严重影响。因此,早期评估重型脑外伤患者的预后对于指导临床治疗、减轻或预防创伤后并发症有重要意义。 S100B蛋白和神经元特异性烯醇化酶是神经特异度、灵敏度较高的生化标志物,其测定方便,可作为评估颅脑损伤严重程度和预后的参考指标。%Severe brain injury and its sequelae can lead to different grades of nervous system dysfunc-tion. The learning and memory dysfunction after traumatic brain injury would significantly impact the quality of life of the patients. Therefore,evaluating the prognosis of severe brain trauma at early stage is of great sig-nificance for guiding the clinical treatment,reducing and preventing the complications after trauma. S100B and neuron-specific enolase as the newly neural specific biochemical markers,can be conveniently detected convenient with high specificity and senstivity,therefore can be used as the reference indexes for assessing the severity and prognosis of brain injury.

  19. Fatores associados à procura de serviços de saúde entre escolares brasileiros: uma análise da Pesquisa Nacional de Saúde do Escolar (PeNSE, 2012

    Directory of Open Access Journals (Sweden)

    Max Moura de Oliveira

    2015-08-01

    Full Text Available Os objetivos foram descrever a procura por serviços/profissionais de saúde por escolares brasileiros e identificar fatores associados. Foram analisados dados da Pesquisa Nacional de Saúde do Escolar 2012; estimadas as prevalências e seus respectivos intervalos de 95% de confiança (IC95% do uso de serviços de saúde entre os escolares. Foi feita regressão logística ajustada por idade. Metade dos estudantes buscaram serviços de saúde; a procura foi maior no sexo feminino; os fatores associados foram: cor branca, escola privada; escolaridade da mãe 12 anos ou mais; ter tido relações sexuais; sofrido ferimento, dor de dentes, tentativa de manter, perder ou ganhar peso, chiado no peito nos últimos 12 meses, ter hábitos de higiene adequados e conhecimento dos pais sobre o que os filhos fazem no tempo livre. A busca por serviços de saúde foi maior no sexo feminino e esteve associada com melhores condições socioeconômicas, presença de sintomas e de comportamentos de risco/proteção.

  20. Expression and Effects of High-Mobility Group Box 1 in Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Xiaoao Pang

    2014-05-01

    Full Text Available We investigated the significance of high- mobility group box1 (HMGB1 and T-cell-mediated immunity and prognostic value in cervical cancer. HMGB1, forkhead/winged helix transcription factor p3 (Foxp3, IL-2, and IL-10 protein expression was analyzed in 100 cervical tissue samples including cervical cancer, cervical intraepithelial neoplasia (CIN, and healthy control samples using immunohistochemistry. Serum squamous cell carcinoma antigen (SCC-Ag was immunoradiometrically measured in 32 serum samples from 37 cases of squamous cervical cancer. HMGB1 and SCC-Ag were then correlated to clinicopathological characteristics. HMGB1 expression tends to increase as cervical cancer progresses and it was found to be significantly correlated to FIGO stage and lymph node metastasis. These findings suggest that HMGB1 may be a useful prognostic indicator of cervical carcinoma. In addition, there were significant positive relationships between HMGB1 and FOXP3 or IL-10 expression (both p < 0.05. In contrast, HMGB1 and IL-2 expression was negatively correlated (p < 0.05. HMGB1 expression may activate Tregs or facilitate Th2 polarization to promote immune evasion of cervical cancer. Elevated HMGB1 protein in cervical carcinoma samples was associated with a high recurrence of HPV infection in univariate analysis (p < 0.05. HMGB1 expression and levels of SCC-Ag were directly correlated in SCC (p < 0.05. Thus, HMGB1 may be a useful biomarker for patient prognosis and cervical cancer prediction and treatment.

  1. Value of elastic strain ratio detection by transvaginal elastography in diagnosis of cervical carcinoma and judgment of illness

    Institute of Scientific and Technical Information of China (English)

    Xu-Sheng Zhang; Chi Zhang; Wei-Jia Zeng

    2016-01-01

    Objective:To find the value of elastic strain ratio detection by transvaginal elastography in diagnosis of cervical carcinoma and judgment of illness.Methods:A total of 178 cases of women receiving vaginal ultrasound in our hospital from August 2011 to June 2014 were selected as research subjects, obtained cervical tissue block received pathological examination, and according to pathological results, all research subjects were divided into cervical carcinoma group 51 cases, benign cervical lesion group 78 cases and healthy control group 49 cases. Elastography pressure release index and elastic strain ratio, protein expression of CDK8, Ki-67, P53, Pin1 and Cyclin D1 in cervical tissue, serum tumor maker levels, serum COX-2, MMP-9, SCCAg, Apo-1/Fas and S-TK1 levels of all subjects were detected, and the correlation between elastic strain ratio and above cervical carcinoma-related indicator values was further analyzed.Results:Elastography pressure release index and elastic strain ratio of cervical carcinoma group were higher than those of benign cervical lesion group and healthy control group; protein expression levels of CDK8, Ki-67, P53, Pin1 and Cyclin D1 in cervical tissue of cervical carcinoma group were higher than those of benign cervical lesion group and healthy control group; serum CA125, Cyfra21-1, CA724 and CEA levels of cervical carcinoma group were higher than those of benign cervical lesion group and healthy control group; serum COX-2, MMP-9, SCCAg, Apo-1/Fas and S-TK1 levels of cervical carcinoma group were higher than those of benign cervical lesion group and healthy control group; elastic strain ratio had positive correlation with protein expression levels of CDK8, Ki-67, P53, Pin1 and Cyclin D1 in cervical tissue as well as CA125, Cyfra21-1, CA724, CEA, COX-2, MMP-9, SCCAg, Apo-1/Fas and S-TK1 levels in serum.Conclusion:Elastic strain ratio detected by transvaginal elastography can be used as the effective means for diagnosis of cervical carcinoma

  2. Clinical Research on Staged Chinese Herbal Medicinal Therapy Combined with Chemotherapy in Treatment of Advanced Non-small Cell Lung Cancer%中医药分阶段结合化疗治疗晚期非小细胞肺癌的临床研究

    Institute of Scientific and Technical Information of China (English)

    郑欢欢

    2016-01-01

    目的:探讨中医药分阶段结合化疗治疗晚期非小细胞肺癌的临床效果。方法纳入的对象为整群选取该院自2011年1月—2013年1月所收治的77例晚期非小细胞肺癌患者,随机分为化疗组、化疗+中医组,观察治疗效果和不良反应等指标。结果(1)化疗+中医组患者临床缓解率72.50%跟化疗组67.57%相似,经χ2检验,P>0.05;(2)治疗后化疗+中医组VEGF、CEA、CYFRA21-1、KPS评分、中位生存期更佳,经t检验,P0.05 (2) After treatment, the VEGF, CEA, CYFRA21-1 and KPS scores and median survival time in the chemotherapy plus Chinese medicine group were better, P<0.05, the medi-an survival time was (10.97±2.72) months in the chemotherapy group and (14.91±2.63) months in the chemotherapy plus Chinese medicine group. (3) The incidence rates of toxic and side effects in the chemotherapy plus Chinese medicine group were obviously lower than those in the chemotherapy group, P<0.05. Nausea and vomiting occurred to 15 cases, diarrhea oc-curred to 6 cases, decrease in hemoglobin occurred to 4 cases and myelosuppression occurred to 4 cases in the chemothera-py group and nausea and vomiting occurred to 7 cases, diarrhea occurred to 2 cases, decrease in hemoglobin occurred to 2 cases and myelosuppression occurred to 2 cases in the chemotherapy plus Chinese medicine group. Conclusion The clinical effect of staged Chinese herbal medicinal therapy combined with chemotherapy in treatment of advanced non-small cell lung cancer is definite, which is worth promotion.

  3. Polarimetric neutron spin echo: Feasibility and first results

    Energy Technology Data Exchange (ETDEWEB)

    Pappas, C. [Hahn-Meitner Institut Berlin, Glienickerstr. 100, 14109 Berlin (Germany)], E-mail: pappas@hmi.de; Lelievre-Berna, E. [Institut Laue-Langevin, 6, Rue Jules Horowitz, 38042 Grenoble (France); Bentley, P. [Hahn-Meitner Institut Berlin, Glienickerstr. 100, 14109 Berlin (Germany); Bourgeat-Lami, E. [Institut Laue-Langevin, 6, Rue Jules Horowitz, 38042 Grenoble (France); Moskvin, E. [Hahn-Meitner Institut Berlin, Glienickerstr. 100, 14109 Berlin (Germany); PNPI, 188300 Gatchina, Leningrad District (Russian Federation); Thomas, M. [Institut Laue-Langevin, 6, Rue Jules Horowitz, 38042 Grenoble (France); Grigoriev, S.; Dyadkin, V. [PNPI, 188300 Gatchina, Leningrad District (Russian Federation)

    2008-07-21

    Neutron Spin Echo (NSE) spectroscopy uses polarized neutrons and accordingly polarization analysis is an intrinsic feature of NSE. However, the multifaceted dynamics of antiferromagnets and helimagnets require more than the classical NSE set-up. Here we present the feasibility test and first results of a new and powerful technique: Polarimetric NSE, obtained by combining the wide angle NSE spectrometer SPAN, developed at HMI with the zero-field polarimeter Cryopad developed at ILL.

  4. MHC-Ⅰ类相关分子A表达在食管癌患者术后自然杀伤细胞免疫治疗中的作用%Role of MHC class Ⅰ-related molecules A on NK cells immunotherapy in esophageal cancer patients after operation

    Institute of Scientific and Technical Information of China (English)

    柳硕岩; 周智锋; 李洁羽; 郑庆丰; 王枫; 陈啸风; 叶韵斌

    2013-01-01

    目的 探讨MHC-Ⅰ类相关分子A(MICA)分子表达与早期食管癌患者术后自然杀伤(NK)细胞过继免疫治疗的相关性,并分析MICA表达在NK细胞治疗中的意义.方法 100例食管癌患者分为单纯手术组40例、MICA阴性NK细胞治疗组(MICA-组)30例及MICA阳性NK细胞治疗组(MICA+组)30例,分别于手术前及手术后60d检测患者外周血CD3+、CD4+、CD4+/CD8+、NK细胞比例、调节性T细胞(Treg细胞)比例、Th1/Th2/Th17细胞因子水平、血清抗体IgA、IgM、IgG以及肿瘤标志物CEA、SCC、CA199、CYFRA21-1等的变化,初步评价患者近期疗效.结果 MICA+组患者外周血CD3+、CD4+、NK细胞阳性率及CD4+/CD8+比例明显高于治疗前[(68.3±7.6)%比(56.2±4.1)%,(39.8±8.2)%比(30.8±4.7)%,(22.2±4.7)%比(18.7±5.5)%,(1.49±0.30)比(1.15±0.61);均P<0.05],Treg细胞阳性率明显低于治疗前[(8.1±4.0)%比(13.4±4.5)%,P<0.05],CD8+细胞阳性率治疗前后差异无统计学意义[(26.9±6.2)%比(27.8±7.1)%,P>0.05].MICA+组患者外周血中Th1类细胞因子(包括IL-2、IFN-γ和TNF-α)水平增高,Th2类细胞因子(IL-4、IL-6、IL-10)水平降低(P<0.05),但Th17细胞因子水平差异无统计学意义(P>0.05).同时提高IgA、IgM、IgG含量,但NK细胞治疗前后肿瘤标志物CEA、SCC、CA199、CYFRA21-1差异无统计学意义(P>0.05).结论 早期食管癌组织MICA表达阳性患者进行NK细胞过继免疫治疗能有效提高细胞免疫及体液免疫水平.%Objective To explore the relevance of expression of MHC class Ⅰ-related molecules A (MICA) molecule and NK cells immunotherapy in esophageal cancer patients after operation.To analyze the significance of MICA expression in NK cell immunotherapy.Methods 100 patients of esophageal cancer were divided into 3 group,surgical alone group,MICA negative with NK therapy group (MICA-group) and MICA positive with NK therapy group (MICA+ group).The immunity indicators and tumor markers

  5. Biomarcadores de cáncer oral en saliva

    Directory of Open Access Journals (Sweden)

    M.V. Madera Anaya

    2013-12-01

    Full Text Available Esta revisión muestra los principales biomarcadores de cáncer oral en saliva. El aspecto clínico y el grado de displasia de las lesiones precancerosas de la cavidad bucal sugieren su capacidad de malignidad; sin embargo, éstas generalmente son diagnosticadas en estadios avanzados, disminuyendo la probabilidad de supervivencia, lo que justifica el diseño de nuevas pruebas diagnósticas que determinen el grado de alteración celular, permitan comprender el proceso degenerativo en el cáncer y establezcan diagnósticos precoz. Esta búsqueda para mejorar los métodos diagnósticos, apunta a que sean sensibles, específicos y menos invasivos, por lo cual el estudio de diferentes biomarcadores en saliva que desde una perspectiva molecular proporcionan información adicional al examen clínico e histopatológico, es considerada como una alternativa eficaz y más cómoda con respecto a los ensayos en sangre. Los biomarcadores que se han descrito en saliva algunos mostrando mayor relación con la carcinogénesis oral son: Ciclina D1, cyfra 21-1, endotelina-1, galectinas 1, 3 y 7, Ki67, lactato deshidrogenasa, metaloproteinasas 2 y 9, proteína p53, proteína de unión a calcio (S100P y telomerasa.

  6. Judgment Value of diagnosis and survival condition of cf-DNA serum level in patients with cervical cancer%血清 cf-DNA 水平对子宫颈癌患者诊断和生存状况的判断价值

    Institute of Scientific and Technical Information of China (English)

    冯达红; 王敏

    2015-01-01

    Objective To investigate the quantitative detection of serum cf-DNA in the diagnosis and survival time of cervical cancer.Methods To take serum samples from 100 patients diagnosed with cervical cancer and 100 healthy con-trols from March 2008 to March 2009 in our hospital.Fluorescence quantitative RT-PCR method was used to detect se-rum cf-DNA content,chemiluminescence was taken to detect the level of serum squamous cell carcinoma antigen (SCC),cytokeratin 19 fragment (CYFRA21-1),carbohydrate antigen 125 (CA125),sugar antigen 19-9 (CA19-9).Re-sults The concentration of serum cf-DNA in cervical cancer patients and healthy controls were 18.3 (0.32-47.5)ng/ml,134.5 (7.9-1721.3 ng/ml),repectively.The concentration of serum cf-DNA in cervical cancer patients was signifi-cantly higher than the control group (Z = - 7.324,P <0.001).ROC curves was used to analysis the diagnostic per-formance of serum cf-DNA in cervical cancer patients with,the area under the curve (0.923)was higher than the tradi-tional serum indicators such as SCC (0.721),CYFRA 21-1(0.734),CA125 (0.652),CA19-9 (0.617).Concentration of serum cf-DNA in patients whose survival time was less than 5-year was significantly lower than it in the the patients whose survival time more than 5 years (Z=6.438,P <0.001).Conclusion The level of serum cf-DNA may benefit the diagnosis and prognosis of cervical cancer.The aided diagnostic value of serum cf-DNA in cervical cancer was better than conventional tumor markers .%目的:探讨血清 cf-DNA 定量检测在宫颈癌辅助诊断及生存状况判断中的价值。方法分别采集2008年3月至2009年3月我院收治的宫颈癌患者及门诊体检健康妇女各100例的血清。运用荧光定量 RT-PCR 方法检测血清 cf-DNA 含量,采用化学发光法检测血清中鳞状细胞癌抗原(SCC)、细胞角蛋白19片段(CYFRA21-1)、糖类抗原125(CA125)、糖类抗原19-9(CA19-9)的含量。结果宫颈癌患者和健康对照者血清 cf

  7. Effect of inducing chemotherapy + chrono-chemotherapy + intensity-modulated radiation therapy on the survival and tumor malignancy in patients with nasopharyngeal carcinoma

    Institute of Scientific and Technical Information of China (English)

    Hui Liu

    2016-01-01

    Objective:To analyze the effect of inducing chemotherapy + chrono-chemotherapy + intensity-modulated radiation therapy on the survival and tumor malignancy in patients with nasopharyngeal carcinoma.Methods: A total of 60 patients with locally advanced nasopharyngeal carcinoma were divided into observation group and control group according to different treatment, observation group received inducing chemotherapy + chrono-chemotherapy + intensity-modulated radiation therapy and control group received conventional treatment. Differences in the survival and tumor malignancy were compared between two groups.Results:miR-143 and miR-218 expression levels in nasopharyngeal carcinoma tissue of observation group after treatment were higher than those of control group, and miR-7 expression level was lower than that of control group; caspase-3, GRP-78 and Bax protein expression levels in tumor tissue of observation group after treatment were higher than those of control group, and Bcl-2 protein expression level was lower than that of control group;serum VEGF, -HBDH, CYFRA21-1 and PCⅢ levels of observation group after treatment were lower than those of control group.Conclusion:Inducing chemotherapy + chrono-chemotherapy + intensity-modulated radiation therapy can reduce the tumor malignancy and optimize the quality of life in patients with nasopharyngeal carcinoma.

  8. Serial Patterns of Ovarian Cancer Biomarkers in a Prediagnosis Longitudinal Dataset

    Directory of Open Access Journals (Sweden)

    Oleg Blyuss

    2015-01-01

    Full Text Available Early detection of ovarian cancer through screening may have impact on mortality from the disease. Approaches based on CA125 cut-off have not been effective. Longitudinal algorithms such as the Risk of Ovarian Cancer Algorithm (ROCA to interpret CA125 have been shown to have higher sensitivity and specificity than a single cut-off. The aim of this study was to investigate whether other ovarian cancer-related biomarkers, Human Epididymis 4 (HE4, glycodelin, mesothelin, matrix metalloproteinase 7 (MMP7, and cytokeratin 19 fragment (CYFRA 21-1, could improve the performance of CA125 in detecting ovarian cancer earlier. Serum samples (single and serial predating diagnosis from 47 women taking part in the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS who went on to develop primary invasive ovarian, fallopian tube, or peritoneal cancer (index cancer (170 samples and 179 matched controls (893 samples were included in the study. A multiplex immunobased assay platform (Becton Dickinson allowing simultaneous measurement of the six serum markers was used. The area under the ROC curve for the panel of three biomarkers (CA125, HE4, and glycodelin was higher than for CA125 alone for all analysed time groups, indicating that these markers can improve on sensitivity of CA125 alone for ovarian cancer detection.

  9. Highly Sensitive Marker Panel for Guidance in Lung Cancer Rapid Diagnostic Units

    Science.gov (United States)

    Blanco-Prieto, Sonia; De Chiara, Loretta; Rodríguez-Girondo, Mar; Vázquez-Iglesias, Lorena; Rodríguez-Berrocal, Francisco Javier; Fernández-Villar, Alberto; Botana-Rial, María Isabel; de la Cadena, María Páez

    2017-01-01

    While evidence for lung cancer screening implementation in Europe is awaited, Rapid Diagnostic Units have been established in many hospitals to accelerate the early diagnosis of lung cancer. We seek to develop an algorithm to detect lung cancer in a symptomatic population attending such unit, based on a sensitive serum marker panel. Serum concentrations of Epidermal Growth Factor, sCD26, Calprotectin, Matrix Metalloproteinases −1, −7, −9, CEA and CYFRA 21.1 were determined in 140 patients with respiratory symptoms (lung cancer and controls with/without benign pathology). Logistic Lasso regression was performed to derive a lung cancer prediction model, and the resulting algorithm was tested in a validation set. A classification rule based on EGF, sCD26, Calprotectin and CEA was established, able to reasonably discriminate lung cancer with 97% sensitivity and 43% specificity in the training set, and 91.7% sensitivity and 45.4% specificity in the validation set. Overall, the panel identified with high sensitivity stage I non-small cell lung cancer (94.7%) and 100% small-cell lung cancers. Our study provides a sensitive 4-marker classification algorithm for lung cancer detection to aid in the management of suspicious lung cancer patients in the context of Rapid Diagnostic Units. PMID:28117344

  10. Noninvasive in vivo monitoring of neuronal differentiation using reporter driven by a neuronal promoter

    Energy Technology Data Exchange (ETDEWEB)

    Hwang, Do Won [Seoul National University, Programs in Neuroscience, Seoul (Korea); Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea); Seoul National University College of Medicine, Laboratory of Molecular Imaging and Therapy of Cancer Research Institute, Seoul (Korea); Kang, Joo Hyun; Lee, Myung Chul [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea); Jeong, Jae Min; Chung, June-Key [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea); Seoul National University College of Medicine, Laboratory of Molecular Imaging and Therapy of Cancer Research Institute, Seoul (Korea); Kim, Soonhag [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea); Seoul National University College of Medicine, Medical Research Center, Seoul (Korea); Lee, Dong Soo [Seoul National University, Programs in Neuroscience, Seoul (Korea); Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul (Korea); Seoul National University Hospital, Department of Nuclear Medicine, Seoul (Korea)

    2008-01-15

    We imaged neuronal differentiation in vivo using dual reporters (sodium iodide symporter [NIS] and luciferase) coupled to a neuron-specific enolase (NSE) promoter. PC12 (NSE positive) and F11 cells were transfected with a bicistronic (NIS and luciferase; pNSE-NF) or a luciferase (pNSE-Fluc) reporter coupled to the NSE promoter. Weak NSE promoter activity was overcome by a two-step transcriptional amplification (TSTA) system (pNSE-TSTA-Fluc). In vivo, NIS and luciferase expression were examined using a {sup 99m}Tc-pertechnetate gamma camera and bioluminescence imaging, respectively. pNSE-NF-transfected PC12 cells showed 3-fold higher radioiodine uptakes and >100-fold higher luciferase activity than parental cells. NIS or luciferase activity was not detected in pNSE-NF-transfected HeLa cells. When F11 cells were differentiated into neurons by db-cAMP, NIS and luciferase activities increased 4-fold compared to those without treatment, which was confirmed by Western blot and RT-PCR of NSE. In vivo in pNSE-NF-transfected F11 cells, db-cAMP treatment increased the luciferase activity but not the scintigraphic activity. In vitro, pNSE-TSTA-Fluc produced 130-fold higher luciferase activity than pNSE-Fluc and neuronal differentiation showed 4-fold higher activity from both pNSE-TSTA-Fluc and pNSE-Fluc than before differentiation. In vivo, in pNSE-TSTA-Fluc-transfected F11 cells, luciferase activity increased after neuronal differentiation. In vivo luciferase activity persisted up to 2 days after db-cAMP-induced neuronal differentiation. NSE promoter-driven dual reporter transgenes revealed the possibility of in vivo imaging of neuronal differentiation, which was further enabled by high amplification using a TSTA system. We propose that this strategy be used to follow the transplanted stem cells during differentiation in live animals. (orig.)

  11. Clinical Application and Progress of Squamous Cell Carcinoma Antigen in Uterine Cervical Neoplasm%鳞状细胞癌抗原在宫颈癌中的临床应用及其研究进展

    Institute of Scientific and Technical Information of China (English)

    赵倩颖

    2013-01-01

    鳞状细胞癌抗原(SCC-Ag)作为特异度的肿瘤标志物,被广泛应用于宫颈鳞状细胞癌(SCC)的早期辅助诊断、预后风险因素评估、手术放化疗疗效评价及复发监测随访中.笔者拟就SCC-Ag在宫颈癌中的临床应用现状及研究进展进行综述如下.

  12. Nursing Student Self-efficacy in Psychomotor Skills: Findings From a Validation, Longitudinal, and Correlational Study.

    Science.gov (United States)

    Bulfone, Giampiera; Fida, Roberta; Ghezzi, Valerio; Macale, Loreana; Sili, Alessandro; Alvaro, Rosaria; Palese, Alvisa

    Student perceptions of self-efficacy (SE) prevent stress and burnout and improve engagement in nursing education, thus increasing learning outcomes. The study aims were to (1) validate a scale measuring nursing SE in psychomotor skills (NSE-PS), (2) describe changes in NSE-PS over time, and (3) explore NSE-PS correlations with burnout and engagement. A total of 1117 nursing students participated. A significant increase in the NSE-PS scores over the years has emerged; in addition, all NSE-PS dimensions were correlated negatively with burnout and positively with engagement.

  13. Study on efficacy -of cisplatin and IL-2 thoracic cavity perfusion combined with DXL chemotherapy in the treatment of non-small cell lung cancer patients with pleural effusion%顺铂、白介素-2胸腔灌注联合多西紫杉醇全身化疗治疗恶性胸腔积液

    Institute of Scientific and Technical Information of China (English)

    向可敏; 李伟; 魏云佳

    2015-01-01

    目的:观察胸腔置管引流并顺铂(DDP)、白介素-2(IL-2)局部灌注联合多西紫杉醇(DXL)静脉化疗治疗非小细胞肺癌( NSCLC)胸腔积液的临床疗效。方法将入选的113例NSCLC胸腔积液患者随机分为观察组(59例)、对照组(54例),两组均予胸腔置管引流,对照组予DDP胸腔灌注并DXL静脉化疗,观察组予DDP+IL-2胸腔灌注并DXL静脉化疗,灌注3~4次,静脉化疗2个周期,对比两组用药毒性反应、肿瘤标志物水平与疗效构成。结果治疗结束后1个月,两组胸腔积液总体疗效构成未见显著性差异( P>0.05);观察组总体有效率(89.8%)、临床获益率(98.3%)均高于对照组(79.6%、92.6%)(P>0.05)。观察组治疗后CEA、CA125、CYFRA21-1均显著低于对照组(P0.05)。结论 DDP胸腔灌注联合DXL静脉化疗治疗 NSCLC引发的胸腔积液基础上,联合IL-2灌注,可在不增加药物毒性反应前提下进一步提高临床疗效。%Objective To observe the clinical efficacy and safety of cisplatin and IL-2 thoracic cavity perfu-sion combined with DXL chemotherapy in the treatment of non-small cell lung cancer patients with pleural effusion. Methods 103 NSCLC patients with pleural effusion were selected and randomly divided into the observation group (59 cases) and the control group (54 cases). All patients were given thoracic cavity catheter drainage guided by B ultrasound, then the control group was given DDP intrapleural perfusion and DXL intravenous chemotherapy, and the observation group was given DDP + IL-2 intrapleural perfusion and DXL intravenous chemotherapy. They were perfused for 3-4 times, and were given 2 cycles of intravenous chemotherapy. Their drug toxicities and clinical effica-cy were compared between the two groups. Results 1 months after the treatment, the clinical efficacy on pleural ef-fusion showed no significant difference between the two groups (Z=1. 426, P=0. 154). The overall effective ratio

  14. Sarcopenia is a novel poor prognostic factor in male patients with pathological Stage I non-small cell lung cancer.

    Science.gov (United States)

    Tsukioka, Takuma; Nishiyama, Noritoshi; Izumi, Nobuhiro; Mizuguchi, Shinjiro; Komatsu, Hiroaki; Okada, Satoshi; Toda, Michihito; Hara, Kantaro; Ito, Ryuichi; Shibata, Toshihiko

    2017-04-01

    Sarcopenia is the progressive loss of muscle mass and strength, and has a risk of adverse outcomes such as disability, poor quality of life and death. As prognosis depends not only on disease aggressiveness, but also on a patient's physical condition, sarcopenia can predict survival in patients with various cancer types. However, its effects on postoperative prognosis in patients with localized non-small cell lung cancers (NSCLC) have never been reported. We retrospectively investigated 215 male patients with pathological Stage I NSCLC. L3 muscle index is defined as the cross-section area of muscle at the third lumbar vertebra level, normalized for height, and is a clinical measurement of sarcopenia. We then investigated the effect of preoperative sarcopenia on their postoperative prognosis. Our 215 subjects included 30 patients with sarcopenia. Sarcopenia was significantly associated with body mass index, nutritional condition, serum CYFRA 21-1 level and pathological stage, but not with preoperative respiratory function or performance status. Frequency of postoperative complications, length of postoperative hospital stay, thoracic drainage period or causes of death were not correlated with the presence of sarcopenia. The sarcopenia group had a significantly shorter median overall survival (32 months) than the no-sarcopenia group. Sarcopenia might not affect short-term outcomes in patients with early-stage lung cancer. Sarcopenia was a predictor of poor prognosis in male patients with Stage I NSCLC. As sarcopenic patients with NSCLC patients are at risk for significantly worse outcomes, their treatments require careful planning, even for those with Stage I disease.

  15. Evaluation of Tumor Markers and Their Impact on Prognosis in Gallbladder, Bile Duct and Cholangiocellular Carcinomas - A Pilot Study.

    Science.gov (United States)

    Liska, Vaclav; Treska, Vladislav; Skalicky, Tomas; Fichtl, Jakub; Bruha, Jan; Vycital, Ondrej; Topolcan, Ondrej; Palek, Richard; Rosendorf, Jachym; Polivka, Jiri; Holubec, Lubos

    2017-04-01

    The behavior of tumor markers in biliary tract malignancies is not well-known and has been scarcely studied. Such markers could play important roles in diagnostic and prognostic schemes as well as in decision-making about the best treatment strategies. This study analyzed the preoperative serum levels of conventional tumor markers (AFP, CEA, CA 19-9, CA 72-4), proliferative marker thymidine kinase (TK) and cytokeratins (TPA, TPS and CYFRA 21.1) in patients with gallbladder carcinoma, bile duct carcinoma (Klatskin) and cholangiocellular carcinoma, in relation to the patient prognosis. The study aimed in finding the role of tumor markers in not properly investigated diseases, where their importance is often marginalized. The study included 43 patients, who underwent either radical surgical procedure (n=21) or explorative laparotomy without any surgical treatment (n=22) for gallbladder carcinoma, bile duct carcinoma (Klatskin tumor) and cholangiocellular carcinoma (24, 8 and 11 patients, respectively) between 2003 and 2010 at our Department. The association of serum tumor markers and patients' prognosis were assessed for the entire cohort and for each cancer type and also with regard to treatment (radical surgery versus explorative laparotomy). Overall survival (OS) and disease-free interval (DFI) were estimated by the Kaplan-Meier method and statistically evaluated using the LogRank test. DFI was computed only in the subgroup of patients treated by radical surgery. The statistical analysis of tumor markers revealed TK as a poor prognostic factor for shorter DFI (HR=3.5, 95%CI=0.6-21.3, ptumor markers for assessment of prognosis (OS or DFI) in patients with gallbladder carcinoma, bile duct carcinoma, and cholangiocellular carcinoma. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  16. NY-ESO-1 autoantibody as a tumor-specific biomarker for esophageal cancer: screening in 1969 patients with various cancers.

    Science.gov (United States)

    Oshima, Yoko; Shimada, Hideaki; Yajima, Satoshi; Nanami, Tatsuki; Matsushita, Kazuyuki; Nomura, Fumio; Kainuma, Osamu; Takiguchi, Nobuhiro; Soda, Hiroaki; Ueda, Takeshi; Iizasa, Toshihiko; Yamamoto, Naoto; Yamamoto, Hiroshi; Nagata, Matsuo; Yokoi, Sana; Tagawa, Masatoshi; Ohtsuka, Seiko; Kuwajima, Akiko; Murakami, Akihiro; Kaneko, Hironori

    2016-01-01

    Although serum NY-ESO-1 antibodies (s-NY-ESO-1-Abs) have been reported in patients with esophageal carcinoma, this assay system has not been used to study a large series of patients with various other cancers. Serum samples of 1969 cancer patients [esophageal cancer (n = 172), lung cancer (n = 269), hepatocellular carcinoma (n = 91), prostate cancer (n = 358), gastric cancer (n = 313), colorectal cancer (n = 262), breast cancer (n = 365)] and 74 healthy individuals were analyzed using an originally developed enzyme-linked immunosorbent assay system for s-NY-ESO-1-Abs. The optical density cut-off value, determined as the mean plus three standard deviations for serum samples from the healthy controls, was fixed at 0.165. Conventional tumor markers were also evaluated in patients with esophageal carcinoma. The positive rate of s-NY-ESO-1-Abs in patients with esophageal cancer (31 %) was significantly higher than that in the other groups: patients with lung cancer (13 %), patients with hepatocellular carcinoma (11 %), patients with prostate cancer (10 %), patients with gastric cancer (10 %), patients with colorectal cancer (8 %), patients with breast cancer (7 %), and healthy controls (0 %). The positive rate of s-NY-ESO-1-Abs was comparable to that of serum p53 antibodies (33 %), squamous cell carcinoma antigen (36 %), carcinoembryonic antigen (26 %), and CYFRA 21-1 (18 %) and gradually increased with the tumor stage. The positive rate of s-NY-ESO-1-Abs was significantly higher in patients with esophageal cancer than in patients with the other types of cancers. On the basis of its high specificity and sensitivity, even in patients with stage I tumors, s-NY-ESO-1-Abs may be one of the first choices for esophageal cancer.

  17. Nanoporous Glass Integrated in Volumetric Bar-Chart Chip for Point-of-Care Diagnostics of Non-Small Cell Lung Cancer.

    Science.gov (United States)

    Li, Ying; Xuan, Jie; Song, Yujun; Qi, Wenjin; He, Bangshun; Wang, Ping; Qin, Lidong

    2016-01-26

    Point-of-care (POC) testing has the potential to enable rapid, low-cost, and large-scale screening. POC detection of a multiplexed biomarker panel can facilitate the early diagnosis of non-small cell lung cancer (NSCLC) and, thus, may allow for more timely surgical intervention for life-saving treatment. Herein, we report the nanoporous glass (NPG) integrated volumetric bar-chart chip (V-Chip) for POC detection of the three NSCLC biomarkers CEA, CYFRA 21-1, and SCCA, by the naked eye. The 3D nanostructures in the NPG membrane efficiently increase the number of binding sites for antibodies and decrease the diffusion distance between antibody and antigen, enabling the low detection limit and rapid analysis time of the NPG-V-Chip. We utilized the NPG-V-Chip to test the NSCLC biomarker panel and found that the limit of detection can reach 50 pg/mL (10-fold improvement over the original V-Chip), and the total assay time can be decreased from 4 to 0.5 h. We then detected CEA in 21 serum samples from patients with common cancers, and the on-chip results showed good correlation with the clinical results. We further assayed 10 lung cancer samples using the device and confirmed the results obtained using conventional ELISA methods. In summary, the NPG-V-Chip platform has the ability of multiplex, low detection limit, low cost, lack of need for accessory equipment, and rapid analysis time, which may render the V-Chip a useful platform for quantitative POC detection in resource-limited settings and personalized diagnostics.

  18. Biomarkers in saliva for the detection of oral squamous cell carcinoma and their potential use for early diagnosis: a systematic review.

    Science.gov (United States)

    Gualtero, Diego F; Suarez Castillo, Angela

    2016-01-01

    Objective To determine the capacity of salivary biomarkers in the early diagnosis of oral squamous cell carcinoma. Study design A systematic review of the literature was performed based on the English titles listed in the PubMed, EBSCO, Cochrane, Science Direct, ISI web Science and SciELO databases using the following search descriptors: Oral cancer, diagnosis, biomarkers, saliva and oral squamous cell carcinoma. Abstracts and full-text articles were assessed independently by two reviewers. International checklists for assessment of methodological quality were used. Levels of evidence and grades of recommendation through the Scottish Intercollegiate Guidelines Network (SIGN) template were recognized. The units of analysis were identified through a reference matrix. Results Through the research strategy and after application of different filters and considering choosing criteria, six studies were obtained for analysis. Salivary biomarkers for oral cancer most frequently found were mRNA and proteins for IL-8, CD44, MMP-1 and MMP-3. New peptide-biomarkers such as Cyfra 21-1 and ZNF510 were found. ZNF 510 was the only biomarker which increased in the population with tumour stage T1 + T2 and T3 + T4. Only one study showed a sensitivity and specificity of 96% when the biomarker ZNF 510 is employed to discriminate early and late tumour stages. Conclusions There is no sufficient scientific evidence to support the capacity of the identified salivary biomarkers for the early diagnosis of oral cancer (sub-clinical stages of the pathogenic period before cancer phenotypes are manifested). Salivary biomarkers, however, may be employed to discriminate between healthy and cancer patients.

  19. Effect of perioperative application ofL-asrginine combined with intacted protein compound preparations on postoperative antitumor immunity and tumor load in patients with gastric cancer

    Institute of Scientific and Technical Information of China (English)

    Xiu-Lan Jiang

    2016-01-01

    Objective:To analyze the effect of perioperative application ofL-arginine combined with intacted protein compound preparations on postoperative antitumor immunity and tumor load in patients with gastric cancer.Methods:A total of 68 patients with gastric cancer received radical operation, and according to different perioperative nutrition intervention, they were divided into control group (normal glucose saline enteral nutrition) and observation group (L-arginine combined with intacted protein compound preparations enteral nutrition) by half. Postoperative short-term antitumor immune cell levels and serum levels of illness-related indexes, nutrition and inflammation indexes of two groups were detected, patients were followed up for 3 years and the gastric stump MRI changes were observed.Results:Venous blood CD4+T lymphocyte level and CD4+/CD8+ ratio of observation group 3 months after treatment were higher than those of control group while CD8+T lymphocyte and Treg cell levels were lower than those of control group; serum Pentraxin-3, CYFRA21-1, TTF-1 and HE4 levels were lower than those of control group; ALB, PA and IL-2 levels were higher than those of control group while IL-6 and IL-10 levels were lower than those of control group (P<0.05). Gastric stump MRI images 3 years after operation were significantly different between two groups.Conclusions:Perioperative application ofL-arginine combined with intacted protein compound preparations can optimize postoperative immune and nutritional state in patients with gastric cancer, and it also has positive effect on reducing the incidence of long-term gastric stump carcinoma and other aspects.

  20. NSE与视频脑电图在儿童热性惊厥的相关研究%THE CORRELATION BETWEEN SERUM NEURON SPECIFIC ENOLASE AND VIDEO EEG IN FEBRILE CONVULSION OF CHILDREN

    Institute of Scientific and Technical Information of China (English)

    刘秀琴; 于树红; 孙若鹏

    2002-01-01

    Objective:To evaluate the diagnostic value of serum neuron specific enolase and Video EEG in Febrile Convulsion of childen. Method:Serum NSE was detected by RIA on the first day and the seventh day after seizure in 40 children with simple febrile convulsion and 18 with complex febrile convulsion. Video EEG was performed at 1st, 7th and 30th day in all the patients. Results: There were significant differences between NSE levels at 24th hour and on 7th day after convulsion (P<0.01). NSE concentrations in patients with SFC and CFC were also different (P < 0.01). The more slowly NSE restored, the higher the abnormal ratio of EGG was . Conclusion: NSE increased in patients within 24hours after seizure, especially in CFC patients. The abnormal ratio of VideoEEG was related to the change of NSE concentration.

  1. Neuronal, non-neuronal and hybrid forms of enolase in brain: structural, immunological and functional comparisons.

    Science.gov (United States)

    Marangos, P J; Zis, A P; Clark, R L; Goodwin, F K

    1978-07-07

    Three forms of the glycolytic enzyme, enolase [2-phospho-D-glycerate hydrolase (E.C. No. 4.2.1.11)] have been prepared from rat whole brain extract. The most acidic enolase form is neuron specific enolase (NSE) which had previously been designated neuron specific protein (NSP). The least acidic form designated non-neuronal enolase (NNE) has been purified and compared structurally, immunologically and functionally to NSE. NNE is a dimer of 86,500 M.W. consistint of two very similar subunits. The data establish that NNE is larger than NSE which has been shown to be composed of two apparently identical 39,000 molecular weight subunits (78,000). NNE is less acidic than NSE having a pI of 5.9 compared to the value of 4.7 for NSE. Structural and immunological analysis establishes that the NNE subunit is distinct from the NSE subunit, and are therfore products of two separate genes. The structural designation of NSE is (gammagamma) and that of NNE (alpha' alpha'). NSE is strictly localized in neurons indicating that the gene coding for the gamma subunit is only expressed in neuronal cells. The intermediate brain enolase form has been partially purified; structural and immunological evidence indicate that it is a hybrid molecule consisting of one NNE subunit and one NSE subunit (alpha'gamma).

  2. Serial measurement of neuron specific enolase improves prognostication in cardiac arrest patients treated with hypothermia: A prospective study

    Directory of Open Access Journals (Sweden)

    Storm Christian

    2012-01-01

    Full Text Available Abstract Background Neuron specific enolase (NSE has repeatedly been evaluated for neurological prognostication in patients after cardiac arrest. However, it is unclear whether current guidelines for NSE cutoff levels also apply to cardiac arrest patients treated with hypothermia. Thus, we investigated the prognostic significance of absolute NSE levels and NSE kinetics in cardiac arrest patients treated with hypothermia. Methods In a prospective study of 35 patients resuscitated from cardiac arrest, NSE was measured daily for four days following admission. Outcome was assessed at ICU discharge using the CPC score. All patients received hypothermia treatment for 24 hours at 33°C with a surface cooling device according to current guidelines. Results The cutoff for absolute NSE levels in patients with unfavourable outcome (CPC 3-5 72 hours after cardiac arrest was 57 μg/l with an area under the curve (AUC of 0.82 (sensitivity 47%, specificity 100%. The cutoff level for NSE kinetics in patients with unfavourable outcome (CPC 3-5 was an absolute increase of 7.9 μg/l (AUC 0.78, sensitivity 63%, specificity 100% and a relative increase of 33.1% (AUC 0.803, sensitivity 67%, specificity 100% at 48 hours compared to admission. Conclusion In cardiac arrest patients treated with hypothermia, prognostication of unfavourable outcome by NSE kinetics between admission and 48 hours after resuscitation may be superior to prognostication by absolute NSE levels.

  3. Fostering multiculturalism: the English Conversation Pairs Program.

    Science.gov (United States)

    Smith, B E; Elander, E

    1995-01-01

    Exposure to and personal interactions with people of diverse cultures foster an acceptance of multiculturalism. To this end, the English Conversation Pairs Program provides weekly conversation sessions between students who are native speakers of English (NSE) and students who speak English as a second language (ESL). Attitudes of twenty-two NSE students are surveyed before and after participation in the Conversation Pairs Program. This paper discusses (a) NSE students' perceptions of the program, (b) perceived changes in cultural sensitivity of NSE students, (c) activities which enhance and/or hamper program effectiveness, and (d) variables which influence program effectiveness.

  4. The relationship between neuron-specific enolase and prognosis of patients with acute traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Yun-yang LIU

    2015-03-01

    Full Text Available Objective To investigate the relationship between neuron-specific enolase (NSE levels in serum and cerebrospinal fluid (CSF of patients with acute traumatic brain injury (TBI and the prognosis of TBI patients.  Methods A total of 89 patients with acute TBI were divided into light, medium, heavy and severe TBI groups based on admission Glasgow Coma Scale (GCS score. Serum NSE expression levels were detected in all cases and NSE levels in CSF were detected in 18 cases within 12 h after TBI. The expression levels of serum NSE in 20 normal people, except cases of lung disease and nervous system damage, were detected as a control group. Results Compared with the control group, serum NSE expression levels of patients in each TBI group were elevated (P < 0.05, for all, and the NSE levels in severe and heavy TBI groups were higher than that in medium and light groups (P < 0.05, for all. The serum NSE expression levels of patients with cerebral contusion were higher than that of patients with diffuse axonal injury (DAI, P = 0.025, subdural hematoma (P = 0.031 and epidural hematoma (P = 0.021. Serum NSE expression levels were negatively correlated with GCS score (rs = - 0.327, P = 0.024 and Glasgow Outcome Scale (GOS score (rs = - 0.252, P = 0.049. The NSE expression levels of CSF in severe and heavy TBI patients were higher than that of serum (P = 0.039, 0.031.  Conclusions NSE expression changes can be evaluated as an auxiliary indicator in reflecting the degree of acute TBI, typing diagnosis and prognostic evaluation, and NSE levels of CSF is more sensitive than that of serum. DOI: 10.3969/j.issn.1672-6731.2015.03.013

  5. The Cloning and Characterization of the Enolase2 Gene of Gekko japonicus and Its Polyclonal Antibody Preparation

    Directory of Open Access Journals (Sweden)

    Mei Liu

    2013-04-01

    Full Text Available The enolase2 gene is usually expressed in mature neurons and also named neuron specific enolase (NSE. In the present study, we first obtained the NSE gene cDNA sequence by using the RACE method based on the expressed sequence tag (EST fragment from the cDNA library of Gekko japonicus and identified one transcript of about 2.2 kb in central nervous system of Gekko japonicus by Northern blotting. The open reading frame of NSE is 1305 bp, which encodes a 435 amino-acid protein. We further investigated the multi-tissue expression pattern of NSE by RT-PCR and found that the expression of NSE mRNA was very high in brain, spinal cord and low in heart, while it was not detectable in other tissues. The real-time quantitative PCR was used to investigate the time-dependent change in the expression of the NSE mRNA level after gecko spinal cord transection and found it significantly increased at one day, reaching its highest level three days post-injury and then decreasing at the seventh day of the experiment. The recombinant plasmid of pET-32a-NSE was constructed and induced to express His fused NSE protein. The purified NSE protein was used to immunize rabbits to generate polyclonal antisera. The titer of the antiserum was more than 1:65536 determined by ELISA. Western blotting showed that the prepared antibody could specifically recognize the recombinant and endogenous NSE protein. The result of immunohistochemistry revealed that positive signals were present in neurons of the brain and the spinal cord. This study provided the tools of cDNA and polyclonal antibody for studying NSE function in Gekko japonicus.

  6. Nonconsensual Sexual Experiences and Alcohol Consumption among Women Entering College

    Science.gov (United States)

    Ross, Lisa Thomson; Kolars, Candace L. Kurth; Krahn, Dean D.; Gomberg, Edith S. Lisansky; Clark, Ginna; Niehaus, Ashley

    2011-01-01

    The authors investigated the relationship between precollege nonconsensual sexual experiences (NSEs) and drinking among women entering college. College women (N = 797) at a midwestern university participated. Eighteen percent reported one or more NSE prior to arriving at college. Having a precollege NSE was associated with recent drinking, binge…

  7. Grænser forudsætter, at man vil bruge magt

    DEFF Research Database (Denmark)

    Østergaard, Uffe

    2016-01-01

    Historisk set. Fra Hadrians mur til de lyseblå gendarmer ved den dansk-tyske grænse: en historie om magt.......Historisk set. Fra Hadrians mur til de lyseblå gendarmer ved den dansk-tyske grænse: en historie om magt....

  8. Sex differences in the vaccine-specific and non-targeted effects of vaccines

    DEFF Research Database (Denmark)

    Flanagan, Katie L; Klein, Sabra L; Skakkebaek, Niels E

    2011-01-01

    Vaccines have non-specific effects (NSE) on subsequent morbidity and mortality from non-vaccine related infectious diseases. Thus NSE refers to any effect that cannot be accounted for by the induction of immunity against the vaccine-targeted disease. These effects are sex-differential, generally ...

  9. Perfluorooctanoic acid rigidifies a model lipid membrane

    NARCIS (Netherlands)

    Brüning, B.A.; Farago, B.

    2014-01-01

    We report a combined dynamic light scattering and neutron spin-echo (NSE) study on vesicles composed of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine under the influence of varying amounts of perfluorooctanoic acid. We study local lipid bilayer undulations using NSE on time

  10. The 'new' self-employed: an issue for social policy?

    NARCIS (Netherlands)

    Westerveld, M.

    2012-01-01

    In this article, an attempt is made to advance the thinking about the ‘new’ self-employed (NSE). This is done by describing four cases which can be considered representative for the ambiguous attitude of social policy makers towards NSE. In the end four conclusions are drawn. The target group is too

  11. Perfluorooctanoic acid rigidifies a model lipid membrane

    NARCIS (Netherlands)

    Brüning, B.A.; Farago, B.

    2014-01-01

    We report a combined dynamic light scattering and neutron spin-echo (NSE) study on vesicles composed of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine under the influence of varying amounts of perfluorooctanoic acid. We study local lipid bilayer undulations using NSE on time scale

  12. A more sensitive radioimmunoassay for neuron-specific enolase suitable for cerebrospinal fluid determinations.

    Science.gov (United States)

    Parma, A M; Marangos, P J; Goodwin, F K

    1981-03-01

    Neuron-specific enolase (NSE) and non-neuronal enolase (NNE) have been shown to be highly specific neuronal and glial products respectively and are therefore useful as biochemical markers of the two major cell types in the vertebrate central nervous system. An iodinated radioimmunoassay (RIA) procedure for human NSE (NSE-H) with approximately 50-fold greater sensitivity than the previously available tritiated assay is described. This assay is capable of detecting 100 pg of NSE-H per assay. NSE levels in human cerebrospinal fluid (CSF) which were previously undetectable with the tritiated RIA are now easily measured and have been shown to be approximately 2 ng/ml of CSF. Furthermore, results obtained with the newly described assay procedure on more concentrated brain tissue extracts are comparable to the tritiated RIA. The iodinated NSE RIA is also shown to be capable of accurately detecting added amounts of NSE in human CSF, indicating the potential clinical usefulness of this assay in determining elevated levels of NSE in CSF.

  13. Art as an Avenue to Science Literacy: Teaching Nanotechnology through Stained Glass

    Science.gov (United States)

    Duncan, Kimberly A.; Johnson, Chris; McElhinny, Kyle; Ng, Steve; Cadwell, Katie D.; Zenner Petersen, Greta M.; Johnson, Angela; Horoszewski, Dana; Gentry, Ken; Lisensky, George; Crone, Wendy C.

    2010-01-01

    Nanoscale science and engineering (NSE) and nanotechnology are emerging fields that have captured the attention of scientists and engineers, as well as mainstream media. However, the general public is relatively unaware of NSE, and schools (both K-12 and undergraduate institutions) rarely include nanotechnology as part of their curriculum. To help…

  14. The In Vivo Antidiabetic Activity of Nigella sativa Is Mediated through Activation of the AMPK Pathway and Increased Muscle Glut4 Content

    Directory of Open Access Journals (Sweden)

    Ali Benhaddou-Andaloussi

    2011-01-01

    Full Text Available The antidiabetic effect of N. sativa seed ethanol extract (NSE was assessed in Meriones shawi after development of diabetes. Meriones shawi were divided randomly into four groups: normal control, diabetic control, diabetic treated with NSE (2 g eq plant/kg or with metformin (300 mg/kg positive control, both administered by daily intragastric gavage for 4 weeks. Glycaemia and body weight were evaluated weekly. At study's end, an Oral Glucose Tolerance Test (OGTT was performed to estimate insulin sensitivity. Upon sacrifice, plasma lipid profile, insulin, leptin, and adiponectin levels were assessed. ACC phosphorylation and Glut4 protein content were determined in liver and skeletal muscle. NSE animals showed a progressive normalization of glycaemia, albeit slower than that of metformin controls. Moreover, NSE increased insulinemia and HDL-cholesterol, compared to diabetic controls. Leptin and adiponectin were unchanged. NSE treatment decreased OGTT and tended to decrease liver and muscle triglyceride content. NSE stimulated muscle and liver ACC phosphorylation and increased muscle Glut4. These results confirm NSE's previously reported hypoglycaemic and hypolipidemic activity. More significantly, our data demonstrate that in vivo treatment with NSE exerts an insulin-sensitizing action by enhancing ACC phosphorylation, a major component of the insulin-independent AMPK signaling pathway, and by enhancing muscle Glut4 expression.

  15. Relationship between serum carcinoembryonic antigen level and epidermal growth factor receptor mutations with the influence on the prognosis of non-small-cell lung cancer patients

    Directory of Open Access Journals (Sweden)

    Cai ZX

    2016-06-01

    Full Text Available Zuxun Cai Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou City, People’s Republic of China Objective: To investigate the relationship between serum carcinoembryonic antigen (CEA level and epidermal growth factor receptor (EGFR gene mutations in non-small-cell lung cancer (NSCLC patients and to analyze the influence of CEA level on postoperative survival time in lung cancer patients. Methods: A total of 296 patients who were treated in Thoracic Surgery Department of Henan Provincial Chest Hospital from September 2011 to September 2013 were recruited. The level of tumor markers, such as CEA, was determined before the surgery, and EGFR gene mutations were detected after surgery. Thereby, the relationship between tumor makers, including CEA, and EGFR mutation and its influence on prognosis could be investigated. Results: Among 296 patients, the positive rate of EGFR gene mutation was 37.84% (112/296; the mutation occurred more frequently in nonsmokers, adenocarcinoma patients, women, and patients aged <60 years (P<0.05. Both tumor markers and chemosensitivity indicators were related to the profile of EGFR mutations. Elevated squamous cell carcinoma and Cyfra21-1 as well as positively expressed ERCC1 were more common in patients with wild-type EGFR (P<0.05, whereas increased CEA level was observed more frequently in patients with EGFR gene mutation (P=0.012. The positive rate of EGFR gene mutations was higher as the serum CEA level increased, that is, the positive rate in patients with serum CEA level <5, 5–20, and >20 µg/L was 39.81%, 45.32%, and 65.47%, respectively (P=0.004. Logistic regression analysis showed that CEA level was an independent factor in predicting EGFR gene mutations, and serum CEA level was also an independent factor in affecting the prognosis of NSCLC patients, as the overall 2-year survival rate was 73.86% in elevated CEA group and 86.43% in normal group (P<0.01. Conclusion: The prognosis of

  16. [Immunosuppressive characteristics of N-stearoylethanolamine a stable compound with cannabimimetic activity].

    Science.gov (United States)

    Hula, N M; Chumak, A A; Mehed', O F; Horid'ko, T M; Kindruk, N L; Berdyshev, A H

    2008-01-01

    Results of investigation of biochemical mechanisms of N-stearoylethanolamine (NSE) influence on the processes of allergic responses of immediate and delayed type (anaphylactic shock in guinea pigs and contact hypersensitivity to 2,4-dinitrochlorobenzene in mice) are presented in the paper. NSE was given per os during two weeks. It was found that in anaphylactic animals, NSE prevented the growth of histamine levels in the heart, kidneys and spleen, suppressed NO2(-) level increase in these organs and promoted its normalization. At the same time NSE prevented the decrease of the level of stable metabolite of nitrogen oxide - nitrite-anion (NO2(-)) in the liver and to a lesser degree in the lungs, and also decreased the activity both inducible and constitutive NO-synthases. NSE normalized the content of TBA-reactive products in the lungs and decreased it in the heart, diminished the decline of activity of glutathione peroxidase, catalase and superoxide dismutase. Effects of NSE depended on its daily dose. About 70% of animals which received NSE in a dose 65 mg/kg of body weight had no fatal outcome after the induction of anaphylactic shock. NSE suppressed the delayed type hypersensitivity response and normalized NO2(-) content in the blood plasma of mice but only at the dose of 50 mg/kg of weight. In the thymus of sensitized mice NSE diminished the content of NO2(-). Thus, though NSE has no affinity for specific CB receptors, in other words, it is not a typical endocannabinoid, its ability to influence the immediate and delayed type allergic reactions opens a perspective for creation of new medications which differ principally from existing pharmacological drugs with anti-allergic and immunosuppressive properties.

  17. A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b) causes nonsyndromic hearing impairment in mice (Mus musculus).

    Science.gov (United States)

    Probst, Frank J; Corrigan, Rebecca R; Del Gaudio, Daniela; Salinger, Andrew P; Lorenzo, Isabel; Gao, Simon S; Chiu, Ilene; Xia, Anping; Oghalai, John S; Justice, Monica J

    2013-01-01

    The study of mouse hearing impairment mutants has led to the identification of a number of human hearing impairment genes and has greatly furthered our understanding of the physiology of hearing. The novel mouse mutant neurological/sensory 5 (nse5) demonstrates a significantly reduced or absent startle response to sound and is therefore a potential murine model of human hearing impairment. Genetic analysis of 500 intercross progeny localized the mutant locus to a 524 kilobase (kb) interval on mouse chromosome 15. A missense mutation in a highly-conserved amino acid was found in the asparagine-linked glycosylation 10B gene (Alg10b), which is within the critical interval for the nse5 mutation. A 20.4 kb transgene containing a wildtype copy of the Alg10b gene rescued the mutant phenotype in nse5/nse5 homozygous animals, confirming that the mutation in Alg10b is responsible for the nse5/nse5 mutant phenotype. Homozygous nse5/nse5 mutants had abnormal auditory brainstem responses (ABRs), distortion product otoacoustic emissions (DPOAEs), and cochlear microphonics (CMs). Endocochlear potentials (EPs), on the other hand, were normal. ABRs and DPOAEs also confirmed the rescue of the mutant nse5/nse5 phenotype by the wildtype Alg10b transgene. These results suggested a defect in the outer hair cells of mutant animals, which was confirmed by histologic analysis. This is the first report of mutation in a gene involved in the asparagine (N)-linked glycosylation pathway causing nonsyndromic hearing impairment, and it suggests that the hearing apparatus, and the outer hair cells in particular, are exquisitely sensitive to perturbations of the N-linked glycosylation pathway.

  18. A point mutation in the gene for asparagine-linked glycosylation 10B (Alg10b causes nonsyndromic hearing impairment in mice (Mus musculus.

    Directory of Open Access Journals (Sweden)

    Frank J Probst

    Full Text Available The study of mouse hearing impairment mutants has led to the identification of a number of human hearing impairment genes and has greatly furthered our understanding of the physiology of hearing. The novel mouse mutant neurological/sensory 5 (nse5 demonstrates a significantly reduced or absent startle response to sound and is therefore a potential murine model of human hearing impairment. Genetic analysis of 500 intercross progeny localized the mutant locus to a 524 kilobase (kb interval on mouse chromosome 15. A missense mutation in a highly-conserved amino acid was found in the asparagine-linked glycosylation 10B gene (Alg10b, which is within the critical interval for the nse5 mutation. A 20.4 kb transgene containing a wildtype copy of the Alg10b gene rescued the mutant phenotype in nse5/nse5 homozygous animals, confirming that the mutation in Alg10b is responsible for the nse5/nse5 mutant phenotype. Homozygous nse5/nse5 mutants had abnormal auditory brainstem responses (ABRs, distortion product otoacoustic emissions (DPOAEs, and cochlear microphonics (CMs. Endocochlear potentials (EPs, on the other hand, were normal. ABRs and DPOAEs also confirmed the rescue of the mutant nse5/nse5 phenotype by the wildtype Alg10b transgene. These results suggested a defect in the outer hair cells of mutant animals, which was confirmed by histologic analysis. This is the first report of mutation in a gene involved in the asparagine (N-linked glycosylation pathway causing nonsyndromic hearing impairment, and it suggests that the hearing apparatus, and the outer hair cells in particular, are exquisitely sensitive to perturbations of the N-linked glycosylation pathway.

  19. The prognostic value of serum neuron-specific enolase in traumatic brain injury: systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Feng Cheng

    Full Text Available BACKGROUND: Several studies have suggested that neuron-specific enolase (NSE in serum may be a biomarker of traumatic brain injury. However, whether serum NSE levels correlate with outcomes remains unclear. The purpose of this review was to evaluate the prognostic value of serum NSE protein after traumatic brain injury. METHODS: PubMed and Embase were searched for relevant studies published up to October 2013. Full-text publications on the relationship of NSE to TBI were included if the studies concerned patients with closed head injury, NSE levels in serum after injury, and Glasgow Outcome Scale (GOS or Extended GOS (GOSE scores or mortality. Study design, inclusion criteria, assay, blood sample collection time, NSE cutoff, sensitivity and specificity of NSE for mortality prediction (if sufficient information was provided to calculate these values, and main outcomes were recorded. RESULTS: Sixteen studies were eligible for the current meta-analysis. In the six studies comparing NSE concentrations between TBI patients who died and those who survived, NSE concentrations correlated with mortality (M.D. 0.28, 95% confidence interval (CI, 0.21 to 0.34; I2 55%. In the eight studies evaluating GOS or GOSE, patients with unfavorable outcomes had significantly higher NSE concentrations than those with favorable outcomes (M.D. 0.24, 95% CI, 0.17 to 0.31; I2 64%. From the studies providing sufficient data, the pooled sensitivity and specificity for mortality were 0.79 and 0.50, and 0.72 and 0.66 for unfavorable neurological prognosis, respectively. The areas under the SROC curve (AUC of NSE concentrations were 0.73 (95% CI, 0.66-0.80 for unfavorable outcome and 0.76 (95% CI, 0.62-0.90 for mortality. CONCLUSIONS: Mortality and unfavorable outcome were significantly associated with greater NSE concentrations. In addition, NSE has moderate discriminatory ability to predict mortality and neurological outcome in TBI patients. The optimal discrimination cutoff

  20. 多梗死性痴呆患者脑脊液肿瘤坏死因子和神经元特异性烯醇化酶的测定及临床意义%Mechanism of changes of tumor necrosis factor and neuron specific enolase in cerebrospinal fluid of patients with multi infarct dementia

    Institute of Scientific and Technical Information of China (English)

    王景周; 高东; 李强; 高唱; 张莉莉

    2002-01-01

    Objective To study the changes of tumor necrosis factor alpha( TNF α )and neuron specific enolase(NSE)in cerebrospinal fluid(CSF)and its clinical significance in patients with multi infarct dementia(MID).Methods The concentration of CSF TNF α was determined by RIA,and the concentration of CSF NSE was determined by ELISA in 55 patients with MID;Compared with Seventy two patients with cerebral infarction(CI)and 32 patients with functional disease of nervous system(control group).Results CSF TNF α or NSE concentration in patients with MID acute episode group was significantly higher than that in control group respectively( P0.05).CSF NSE concentration in MID stationary phase group was lower than that in control group( P0.05).Conclusion CSF TNF α and NSE concentration of all patients with MID did not present concordant changes at all.The changes of CSF TNF α and NSE concentrations could respond the brain neuroendocrine and immunological changes objectively in patients with MID.However,the changes of TNF α,NSE could not directly indicate the intellectual status of MID patients;for it was not a kind of intellectual factor.

  1. The existence and neurobiological significance of neuronal and glial forms of the glycolytic enzyme enolase.

    Science.gov (United States)

    Marangos, P J; Schmechel, D; Zis, A P; Goodwin, F K

    1979-08-01

    The isoenzymes of the glycolytic enzyme enolase have been separated and purified. The structural and functional properties of two brain enolases are described. Immunocytochemical techniques have established that one brain enolase is restricted to neuronal cells (neuron-specific enolase, NSE) while the other is localized in glial cells (nonneuronal enolase, NNE). The brain enolases, therefore, represent the first example of functional markers for neuronal and glial cell types in brain. The two enzymes are structurally distinct with the evidence establishing that they are products of separate genes. Functionally, the neuronal enolase has been demonstrated to be uniquely stable to concentrations of chloride salts that rapidly inactivate the glial enzyme. NSE may therefore represent an adaptation of this enzyme that is specifically suited to the neuronal milieu. A specific radioimmunoassay is described for NNE and NSE with the studies reported indicating that neuronal enzyme levels vary considerably when different brain areas are compared, suggesting a relationship between functional activity and levels of NSE. In addition to being a marker for neuronal cells, NSE has also been found to be present in various glands. The cells of the APUD series (amine precursor uptake and decarboxylation cells) in the pituitary, adrenal medulla, pineal, thyroid, and pancreas have been shown to contain NSE. NSE is, therefore, also a marker for these neuronlike endocrine cells since they are the only cells other than neurons that contain this protein.

  2. Nursing students' confidence in medication calculations predicts math exam performance.

    Science.gov (United States)

    Andrew, Sharon; Salamonson, Yenna; Halcomb, Elizabeth J

    2009-02-01

    The aim of this study was to examine the psychometric properties, including predictive validity, of the newly-developed nursing self-efficacy for mathematics (NSE-Math). The NSE-Math is a 12 item scale that comprises items related to mathematic and arithmetic concepts underpinning medication calculations. The NSE-Math instrument was administered to second year Bachelor of Nursing students enrolled in a nursing practice subject. Students' academic results for a compulsory medication calculation examination for this subject were collected. One-hundred and twelve students (73%) completed both the NSE-Math instrument and the drug calculation assessment task. The NSE-Math demonstrated two factors 'Confidence in application of mathematic concepts to nursing practice' and 'Confidence in arithmetic concepts' with 63.5% of variance explained. Cronbach alpha for the scale was 0.90. The NSE-Math demonstrated predictive validity with the medication calculation examination results (p=0.009). Psychometric testing suggests the NSE-Math is a valid measure of mathematics self-efficacy of second year nursing students.

  3. Admission plasma levels of the neuronal injury marker neuron-specific enolase are associated with mortality and delirium in sepsis.

    Science.gov (United States)

    Anderson, Brian J; Reilly, John P; Shashaty, Michael G S; Palakshappa, Jessica A; Wysoczanski, Alex; Dunn, Thomas G; Kazi, Altaf; Tommasini, Anna; Mikkelsen, Mark E; Schweickert, William D; Kolson, Dennis L; Christie, Jason D; Meyer, Nuala J

    2016-12-01

    Neuron-specific enolase (NSE) concentrations are prognostic following traumatic and anoxic brain injury and may provide a method to quantify neuronal injury in other populations. We determined the association of admission plasma NSE concentrations with mortality and delirium in critically ill septic patients. We performed a retrospective analysis of 124 patients from a larger sepsis cohort. Plasma NSE was measured in the earliest blood draw at intensive care unit admission. Primary outcomes were 30-day mortality and intensive care unit delirium determined by chart review. Sixty-one patients (49.2%) died within 30 days, and delirium developed in 34 (31.5%) of the 108 patients who survived at least 24 hours and were not persistently comatose. Each doubling of the NSE concentration was associated with a 7.3% (95% confidence interval [CI] 2.5-12.0, P= .003) increased risk of 30-day mortality and a 5.2% (95% CI 3.2-7.2, Pdelirium. An NSE concentration >12.5 μg/L was independently associated with a 23.3% (95% CI 6.7-39.9, P= .006) increased risk of 30-day mortality and a 29.3% (95% CI 8.8-49.8, P= .005) increased risk of delirium. Higher plasma NSE concentrations were associated with mortality and delirium in critically ill septic patients, suggesting that NSE may have utility as a marker of neuronal injury in sepsis. Copyright © 2016 Elsevier Inc. All rights reserved.

  4. Correlation between serum neuron specific enolase and functional neurological outcome in patients of acute ischemic stroke

    Directory of Open Access Journals (Sweden)

    Sana Zaheer

    2013-01-01

    Full Text Available Context: The use of biomarkers to predict stroke prognosis is gaining particular attention nowadays. Neuron specific enolase (NSE, which is a dimeric isoenzyme of the glycolytic enzyme enolase and is found mainly in the neurons is one such biomarker. Aims: This study was carried out on patients of acute ischemic stroke with the aims to determine the correlation between NSE levels on the day of admission with infarct volume, stroke severity, and functional neurological outcome on day 30. Materials and Methods: Seventy five patients of acute ischemic stroke admitted in the Department of Medicine were included in the study. Levels of NSE were determined on day 1 using the human NSE ELISA kit (Alpha Diagnostic International Texas 78244, USA. Volume of infarct was measured by computed tomography (CT scan using the preinstalled software Syngo (version A40A of Siemen′s medical solutions (Forchheim, Germany. Stroke severity at admission was assessed using Glasgow coma scale (GCS and functional neurological outcome was assessed using modified Rankin scale (mRS on day 30. Statistical Analysis Used: Statistical analysis was performed using the SPSS software for windows version 15.0 (SPSS. Results: A positive correlation was found between concentration of NSE on day 1 and infarct volume determined by CT scan (r = 0.955, P < 0.001. A strong negative correlation was found between GCS at presentation and concentration of NSE on day 1 (r = −0.806, P < 0.001. There was a positive correlation between NSE levels at day 1 and functional neurological outcome assessed by mRS at day 30 (r = 0.744, P < 0.001. Conclusions: Serum levels of NSE in first few days of ischemic stroke can serve as a useful marker to predict stroke severity and early functional outcome. However, larger studies with serial estimation of NSE are needed to establish these observations more firmly.

  5. Neuron-Specific Enolase Is Elevated in Asymptomatic Carriers of Leber's Hereditary Optic Neuropathy

    Science.gov (United States)

    Yee, Kenneth M.; Ross-Cisneros, Fred N.; Lee, Jeong Goo; Da Rosa, Arlon Bastos; Salomao, Solange R.; Berezovsky, Adriana; Belfort, Rubens; Chicani, Filipe; Moraes-Filho, Milton; Sebag, Jerry; Carelli, Valerio; Sadun, Alfredo A.

    2012-01-01

    Purpose. Neuron-specific enolase (NSE) is a biomarker for neuronal stress. Leber's hereditary optic neuropathy (LHON) is a mitochondrial disease affecting retinal ganglion cells (RGC). These RGCs and their axons in the retinal nerve fiber layer (RNFL) and optic nerve head may show subclinical pathology in unaffected mutation carriers, or undergo cell death in affected patients. We hypothesize that increased levels of blood NSE may characterize LHON carriers as a biomarker of ongoing RGC stress. Methods. Serum was obtained from 74 members of a Brazilian pedigree with LHON carrying the homoplasmic 11778/ND4 mitochondrial DNA mutation. Classified by symptoms and psychophysical metrics, 46/74 patients were unaffected mutation “carriers,” 14/74 were “affected,” and 14/74 were “off-pedigree” controls. Serum NSE levels were determined by ELISA specific for the γ subunit of NSE. Results. Serum NSE concentrations in carriers (27.17 ± 39.82 μg/L) were significantly higher than affected (5.66 ± 4.19 μg/L; P = 0.050) and off-pedigree controls (6.20 ± 2.35 μg/L; P = 0.047). Of the 14/46 (30.4 %) carriers with significantly elevated NSE levels (mean = 75.8 ± 42.3 μg/L), 9/14 (64.3%) were male. Furthermore, NSE levels were nearly three times greater in asymptomatic male carriers (40.65 ± 51.21 μg/L) than in asymptomatic female carriers (15.85 ± 22.27 μg/L; P = 0.034). Conclusions. Serum NSE levels are higher in LHON carriers compared with affected and off-pedigree individuals. A subgroup of mostly male carriers had significantly elevated serum NSE levels. Thus, male carriers are at higher risk for LHON-related neuronal stress. PMID:22893673

  6. Neuron-specific Enclose and Myelin Basic Protein in Cerebrospinal Fluid of Patients with First Episode Schizophrenia

    Institute of Scientific and Technical Information of China (English)

    LI Shuying; WU Hanrong; GUO Huirong; ZHAO Zheng

    2006-01-01

    In order to study whether patients with schizophrenia have cerebral injury, neuron-specific enolase (NSE) and myelin basic protein (MBP)in cerebrospinal fluid (CSF) of 33 patients with first episode schizophrenia and 9 from the control group were determined by double antibody sandwich enzyme immunoassay method. The results showed that there was significant difference in the NSE contents between the experimental group and control group (P<0.01). The NSE contents in CSF in the experimental group were positively correlated with MBP in schizophrenia patients (P<0.05). These findings suggested that patients with schizophrenia had cerebral injury.

  7. The expression of serum YKL-40 in patients with cervical cancer and its clinical value%软骨糖蛋白-39在宫颈癌患者外周血的表达及临床意义

    Institute of Scientific and Technical Information of China (English)

    曹丽群

    2016-01-01

    Objective Our study was aimed to analyze the expression of serum YKL - 40 in patients with cervical cancer and its clinical value. Methods Clinical data of patients with cervical cancer(taken pathological findings as a golden standard)received treatment at our hospital from 2013 to 2015. All these patients were included into the present study as Group A and were analyzed. 50 healthy cases were en-rolled as Group B. Serum tumor related markers were tested and compared between the two groups. YKL - 40 in the diagnosis of cervical cancer was analyzed by ROC curve. Results Group A patients had higher levels of SCC - Ag,CEA and YKL - 40 than these of Group B,the difference were statistically significant( P < 0. 05). ROC analysis showed that the YKL - 40 cut - off point value of 87. 91 ng/ ml. We can acquire a sensi-tivity of 91. 11% ,a specificity of 90. 00% ,an AUC of 0. 91,87. 91 ng/ ml used for the diagnosis of cervical cancer. These indexes were better than these of CEA or SCC - Ag. Correlation analysis showed that the levels of YKL - 40 had significant positive correlation with SCC - Ag and CEA for Group A patients( P < 0. 05). For Group A patients,TNM - Ⅱ patients had a higher levels of serum YKL - 40 than that of TNM - Ⅰpatients,the difference was statistically significant(177. 6 ± 22. 6 vs. 127. 5 ± 38. 4;t = 4. 869,P < 0. 05). Conclusion The levels of serum YKL - 40 increased significantly in patients with cervical cancer,which showed a certain diagnostic value for cervical cancer.%目的:观察软骨糖蛋白-39(YKL -40)在宫颈癌患者外周血的表达并分析其临床意义,以期为此类患者的诊疗提供参考。方法分析2013年1月至2015年1月接受治疗的45例宫颈癌患者(以病理诊断为金标准)的临床资料,列为观察组。另纳入50例健康体检者作为对照组。对比两组外周血肿瘤相关标志物及 YKL -40水平。采用ROC 曲线分析 YKL -40在诊断宫颈癌中的价值。结果观察

  8. How Are Lung Carcinoid Tumors Diagnosed?

    Science.gov (United States)

    ... for chromogranin A (CgA), neuron-specific enolase (NSE), cortisol , and substance P . Depending on where the tumor ... Cancer Atlas Press Room Cancer Statistics Center Volunteer Learning Center Follow Us Twitter Facebook Instagram Cancer Information, ...

  9. Self-focusing of whistler waves

    Science.gov (United States)

    Karpman, V. I.; Kaufman, R. N.; Shagalov, A. G.

    1992-01-01

    The theory of axially symmetric self-focusing of whistler waves, based on the full system of Maxwell equations, is developed. The plasma is described by the magnetohydrodynamic equations including the ponderomotive force from RF field. The nonlinear Schrodinger equations (NSE) for arbitrary azimuthal modes of whistler waves are derived. It is shown that they differ from the NSE for a scalar field; this is connected with an intrinsic angular momentum due to the rotating polarization of whistlers. It is shown that the self-focusing, as described by the NSE, differs in its final stage from the results following the full set of Maxwell equations. The latter gives defocusing after sufficient narrowing of the initial wave beam, due to transformation of the trapped wave into a nontrapped branch which is not contained in the NSE description. The oscillatory character of the defocusing is demonstrated.

  10. Sex differences in the vaccine-specific and non-targeted effects of vaccines

    DEFF Research Database (Denmark)

    Flanagan, Katie L; Klein, Sabra L; Skakkebaek, Niels E

    2011-01-01

    Vaccines have non-specific effects (NSE) on subsequent morbidity and mortality from non-vaccine related infectious diseases. Thus NSE refers to any effect that cannot be accounted for by the induction of immunity against the vaccine-targeted disease. These effects are sex-differential, generally...... being more pronounced in females than males. Furthermore, the NSE are substantial causing greater than fifty percent changes in all cause mortality in certain settings, yet have never been systematically tested despite the fact that millions of children receive vaccines each year. As we strive...... to eliminate infectious diseases through vaccination programmes, the relative impact of NSE of vaccines on mortality is likely to increase, raising important questions regarding the future of certain vaccine schedules. A diverse group of scientists met in Copenhagen to discuss non-specific and sex...

  11. sexual abuse in adolescents - data from a psychiatric treatment ...

    African Journals Online (AJOL)

    psychiatric treatment centre for adolescents with emotional and behavioural problems. ... between sexual abuse and depresSIon, suicidal symptoms and alcohol nse is ... severe conduct disorder and/or psychotic illness are referred elsewhere.

  12. Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients

    National Research Council Canada - National Science Library

    Warzynski, Michael J; Graham, David M; Axtell, Richard A; Higgins, James V; Hammers, Yuki A

    2002-01-01

    .... Following the “rare event” philosophy of selecting one negative and two positive antigens, we initially tried a “cocktail” of CD45 − CD56 very bright+ neuron‐specific enolase (NSE) cytoplasmic...

  13. President Ilves meets with German Chancellor : "The democratic countries are at a decision-making threshold"

    Index Scriptorium Estoniae

    2008-01-01

    President Toomas Hendrik Ilvese kohtumisest Saksamaa liidukantsler Angela Merkeliga, kes saabus Eestisse ühepäevasele ametlikule visiidile. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu

  14. Detection of neuron specific enolase concentrations in cerebrospinal fluid from patients with neurological disorders by means of a sensitive enzyme immunoassay.

    Science.gov (United States)

    Vermuyten, K; Lowenthal, A; Karcher, D

    1990-02-28

    An enzyme linked immunosorbent assay (ELISA) for the detection of neuron specific enolase (NSE) in cerebrospinal fluid (CSF) was developed. The sensitivity of the ELISA was less than 1 microgram/ml. This sensitivity is comparable with radioimmunoassays which have the disadvantage that radiolabelled products are used. The developed assay was used to measure cerebrospinal fluid neuron specific enolase (CSF-NSE) levels in 1178 patients with neurological disorders to establish its potential usefulness and clinical application. CSF-NSE levels in this group of patients were independent of sex and no correlation with age was found. CSF-NSE was significantly increased in Creutzfeldt-Jacob disease, meningeal hemorrhage, thrombosis, Guillain-Barré syndrome and in schizophrenia.

  15. "Kõige selgem õiguslik järjepidevus valitses Eestimaa kubermangus" : ühe baltisaksa ajalookirjutuse paradigma kriitika / Andres Andresen

    Index Scriptorium Estoniae

    Andresen, Andres, 1971-

    2013-01-01

    Õiguslikust järjepidevusest tuleneva õiguspärasuse printsiibi vastandumisest riigivõimu suveräänse seadusandluse printsiibile. Eestimaa kirikukorralduse põhijoontest kuni 1710. aastani ja muudatustest pärast seda.

  16. Actitud de Marcha según Nivel Socioeconómico en Adultos Mayores Autovalentes

    Directory of Open Access Journals (Sweden)

    Paul Medina González

    Full Text Available RESUMEN La marcha es fundamental para la funcionalidad de adultos mayores (AM, además su expresión actitudinal se relaciona con diferentes estados emocionales, los cuales son condicionados por el nivel socioeconómico (NSE. El propósito de esta investigación fue evaluar el comportamiento de atributos actitudinales de marcha según el NSE en AM autovalentes. Participaron este estudio 71 AM autovalentes de la comunidad de Talca-Chile, categorizados según el NSE a los cuales se les aplicó el instrumento denominado análisis de forma y esfuerzo de la marcha previo caracterización de confiabilidad. Los resultados indican que el estilo de marcha no es diferente según el NSE en AM autovalentes; sin embargo, los perfiles cualificados presentan variaciones en los anclajes las que son dependientes del género.

  17. "Kõige selgem õiguslik järjepidevus valitses Eestimaa kubermangus" : ühe baltisaksa ajalookirjutuse paradigma kriitika / Andres Andresen

    Index Scriptorium Estoniae

    Andresen, Andres, 1971-

    2013-01-01

    Õiguslikust järjepidevusest tuleneva õiguspärasuse printsiibi vastandumisest riigivõimu suveräänse seadusandluse printsiibile. Eestimaa kirikukorralduse põhijoontest kuni 1710. aastani ja muudatustest pärast seda.

  18. President Ilves meets with German Chancellor : "The democratic countries are at a decision-making threshold"

    Index Scriptorium Estoniae

    2008-01-01

    President Toomas Hendrik Ilvese kohtumisest Saksamaa liidukantsler Angela Merkeliga, kes saabus Eestisse ühepäevasele ametlikule visiidile. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu

  19. Main - RPSD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us RPSD...nse Update History of This Database Site Policy | Contact Us Main - RPSD | LSDB Archive ...

  20. Challenges in neutron spin echo spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Pappas, C., E-mail: c.pappas@tudelft.n [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); Faculty of Applied Sciences, Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands); Lelievre-Berna, E.; Falus, P.; Farago, B. [Institut Laue Langevin, 6 rue Jules Horowitz, 38042 Grenoble (France); Bentley, P. [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); Institut Laue Langevin, 6 rue Jules Horowitz, 38042 Grenoble (France); Moskvin, E. [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); PNPI, 188300 Gatchina, Leningrad District (Russian Federation); Krist, Th. [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); Grigoriev, S. [PNPI, 188300 Gatchina, Leningrad District (Russian Federation)

    2009-09-01

    With the new brilliant neutron sources and the developments of novel optical elements, neutron spin echo (NSE) spectroscopy evolves to tackle new problems and scientific fields. The new developments pave the way to complex experimental set-ups such as the intensity modulated variant of NSE (IMNSE), a powerful technique which was introduced some 20 years ago but found limited use up to now. With the new compact supermirror or He{sup 3} polarizers IMNSE becomes attractive for a broad range of applications in magnetism, soft matter and biology. A novel development along this line is the polarimetric NSE technique, which combines IMNSE and the zero-field polarimeter Cryopad to access components of the scattered polarization that are transverse to the incoming polarization. Polarimetric NSE is the method of choice for studying chiral fluctuations, as illustrated by new results on the reference helimagnet MnSi.

  1. Volume and surface contributions to the nuclear symmetry energy within the coherent density fluctuation model

    CERN Document Server

    Antonov, A N; Sarriguren, P; de Guerra, E Moya

    2016-01-01

    The volume and surface components of the nuclear symmetry energy (NSE) and their ratio are calculated within the coherent density fluctuation model (CDFM). The estimations use the results of the model for the NSE in finite nuclei based on the Brueckner energy-density functional for nuclear matter. In addition, we present results for the NSE and its volume and surface contributions obtained by using the Skyrme energy-density functional. The CDFM weight function is obtained using the proton and neutron densities from the self-consistent HF+BCS method with Skyrme interactions. We present and discuss the values of the volume and surface contributions to the NSE and their ratio obtained for the Ni, Sn, and Pb isotopic chains studying their isotopic sensitivity. The results are compared with estimations of other approaches which have used available experimental data on binding energies, neutron-skin thicknesses, excitation energies to isobaric analog states (IAS) and also with results of other theoretical methods.

  2. Workshop on neutron spin-echo

    Energy Technology Data Exchange (ETDEWEB)

    Aynajian, P.; Habicht, K.; Keller, Th.; Keimer, B.; Mezei, F.; Monkenbusch, M.; Allgaier, J.; Richter, D.; Fetters, L.J.; Muller, K.; Kreiling, S.; Dehnicke, K.; Greiner, A.; Ehlers, G.; Arbe, A.; Colmenero, J.; Richter, D.; Farago, B.; Monkenbusch, M.; Ohl, M.; Butzek, M.; Kozielewski, T.; Monkenbusch, M.; Richter, D.; Pappas, C.; Hillier, A.; Manuel, P.; Cywinski, R.; Bentley, P.; Alba, M.; Mezei, F.; Campbell, I.A.; Zimmermann, U.; Ellis, J.; Jobic, H.; Pickup, R.M.; Pappas, C.; Farago, B.; Cywinski, R.; Haussler, W.; Holderer, O.; Frielinghaus, H.; Byelov, D.; Monkenbusch, M.; Allgaier, J.; Richter, D.; Egger, H.; Hellweg, Th.; Malikova, N.; Cadene, A.; Marry, V.; Dubois, E.; Turq, P.; Gardner, J.S.; Ehlers, G.; Bramwell, St.S.; Grigoriev, S.; Kraan, W.; Rekveldt, T.; Bouwman, W.; Van Dijk, N.; Falus, P.; Vorobiev, A.; Major, J.; Felcher, G.P.; Te-velthuis, S.; Dosch, H.; Vorobiev, A.; Dridi, M.H.; Major, J.; Dosch, H.; Falus, P.; Felcher, G.P.; Te Velthuis, S.G.E.; Bleuel, M.; Broell, M.; Lang, E.; Littrell, K.; Gahler, R.; Lal, J.; Lauter, H.; Toperverg, B.; Lauter, V.; Jernenkov, M.; Stueber, S.; Enderle, M.; Janoschek, M.; Keller, Th.; Klimko, S.; Boeni, P.; Nagao, M.; Yamada, N.; Kawabata, Y.; Seto, H.; Takeda, T.; Yoshizawa, H.; Yoshida, K.; Yamaguchi, T.; Bellissent-Funel, M.C.; Longeville, St

    2005-07-01

    This document gathers the abstracts of most papers presented at the workshop. Neutron spin-echo (NSE) spectroscopy is a well established technique with a growing expert user community, the aim of the meeting was to discuss the latest achievements in neutron spin-echo science and instrumentation. One of the applications presented is the investigation on the microscopic scale of the dynamics of water in montmorillonite clays with Na{sup +} and Cs{sup +} ions in monolayer and bilayer states. The NSE technique has been used in the normal and resonance modes. NSE results show consistently slower dynamics (higher relaxation times) than both time-of-flight technique (TOF) and classical molecular dynamics simulations (MD). In the present TOF and NSE experiments, anisotropy of the water motion in the interlayer is almost impossible to detect, due to the use of powder samples and insufficient resolution. (A.C.)

  3. The Level Of Corporate Dividend Payout To Stockholders: Does Optimal Dividend Policy Exist For Firms Quoted At The Nairobi Stock Exchange?

    National Research Council Canada - National Science Library

    Bitok Kibet; Tenai Joel; Cheruiyot Thomas; Maru Loice; Kipsat Mary

    2010-01-01

    The objective of this study was to determine the level of corporate dividend payout to stockholders and establish if the optimal dividend policy exists for the firms quoted at the Nairobi Stock Exchange (NSE...

  4. Disease: H01114 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available oma of optic nerve (COLON); Optic nerve hypoplasia Ocular coloboma is a congenital and common malformation w...nse mutations are related to foveal hypoplasia, congenital cataracts, or anterior segment anomalies. Develop

  5. Estres materno y configuracion familiar: estudio comparativo en familias chilenas monoparentales y nucleares de bajos ingresos

    National Research Council Canada - National Science Library

    Olhaberry, Marcia; Farras, Chamarrita

    2012-01-01

    .... Se estudian los niveles de estres materno en familias chilenas nucleares y monoparentales de NSE bajo, considerando el estres en distintas dimensiones, asociado al rol materno, a la interaccion madre...

  6. Sequence Classification: 523339 [

    Lifescience Database Archive (English)

    Full Text Available nse regulator in two-component regulatory system with PhoQ, transcribes genes expressed under low Mg+ concentration (OmpR family) || http://www.ncbi.nlm.nih.gov/protein/62179752 ...

  7. Effects of sevoflurane and propofol on S100β and neuron‑specific ...

    African Journals Online (AJOL)

    2015-06-10

    Jun 10, 2015 ... indicating cerebral cell injury. The purpose of the present ... S100β protein and neuron‑specific enolase (NSE) have been evaluated as valid .... This study was designed with the same patient numbers as. Kanbak's study.[16].

  8. Aldersgrænser bør være fortid

    DEFF Research Database (Denmark)

    Thomsen, Steen

    2013-01-01

    Vi har ikke råd til at undvære mennesker, der både kan og vil, på grund af en absurd aldersgrænse.......Vi har ikke råd til at undvære mennesker, der både kan og vil, på grund af en absurd aldersgrænse....

  9. Secretagogin is a new neuroendocrine marker in the human prostate

    DEFF Research Database (Denmark)

    Adolf, Katja; Wagner, Ludwig; Bergh, Anders

    2007-01-01

    marker in carcinoid tumors of the lung and the gastrointestinal tract. The present study analyzes the expression of secretagogin in normal and malign prostate tissue. METHODS: We analyzed immunoreactivity for secretagogin, chromogranin A (CgA), neuron specific enolase (NSE), and synaptophysin (SYN...... and co-localized with the NE markers CgA and NSE. The expression of secretagogin is significantly correlated to CgA (P epithelium (P

  10. Percepciones sobre disponibilidad de alimentos y autorreporte de ingesta alimentaria en mujeres urbanas costarricenses: Un estudio piloto

    Directory of Open Access Journals (Sweden)

    Traci A. Bekelman

    2016-01-01

    Full Text Available Antecedentes: Se considera que la disponibilidad y consumo de alimentos han contribuido al aumento de obesidad entre las mujeres de bajo nivel socioeconómico (NSE en Latinoamérica. En Costa Rica, pocos estudios han investigado cómo la disponibilidad y consumo varían según NSE. Objetivos: Los objetivos de este estudio en San José, Costa Rica, son (1 evaluar percepciones sobre la disponibilidad de alimentos y (2 describir el consumo de alimentos en mujeres que viven en áreas de alto y bajo NSE. Métodos: En marzo 2013, se reclutaron 30 mujeres no embarazadas, ni dando lactancia materna, de 25 a 50 años, en un cantón de bajo y otro de alto NSE. Las percepciones sobre disponibilidad de alimentos se analizaron de manera cualitativa mediante entrevistas estructuradas a 17 de las 30 mujeres. El consumo de alimentos se analizó de manera cuantitativa mediante recordatorios de 24 horas en las 30 mujeres. Resultados: Muchas mujeres del cantón de bajo NSE reportaron que incluirían más pollo y pescado en su dieta si el costo no fuera una barrera. La ingesta de proteína como proporción del valor energético total fue significativamente mayor en las mujeres del cantón de alto NSE (17% DE=5 vs. 13 DE=3, p = 0.02. La ingesta de proteína de origen animal fue significativamente mayor entre las mujeres del cantón de alto NSE (38 g DE=22 vs. 21 DE=11, p=0.02. Conclusión: La ingesta de proteína fue mayor en las mujeres de alto NSE y puede deberse al costo percibido de los alimentos de origen animal.

  11. Optimization of Bow-Bulb Forms for Resistance and Seakeeping Characteristics.

    Science.gov (United States)

    2014-09-26

    Johns , NSE Department Dr. Bruce Nehrling, NSE Depa ent Accepted for Trident Scnolar Committee Chairman Date...hull form. It is defined as the volume of displacement divided by the product of length, beam, * iand draft: CB = VOLUME ," L X B X TLXBX...speed of a snip. It is equal to the ship’s speed divided by the square root of the product of the gravitational constant times the ship’s length: I *N

  12. Correlation between serum neuron specific enolase and functional neurological outcome in patients of acute ischemic stroke.

    Science.gov (United States)

    Zaheer, Sana; Beg, Mujahid; Rizvi, Imran; Islam, Najmul; Ullah, Ekram; Akhtar, Nishat

    2013-10-01

    The use of biomarkers to predict stroke prognosis is gaining particular attention nowadays. Neuron specific enolase (NSE), which is a dimeric isoenzyme of the glycolytic enzyme enolase and is found mainly in the neurons is one such biomarker. This study was carried out on patients of acute ischemic stroke with the aims to determine the correlation between NSE levels on the day of admission with infarct volume, stroke severity, and functional neurological outcome on day 30. Seventy five patients of acute ischemic stroke admitted in the Department of Medicine were included in the study. Levels of NSE were determined on day 1 using the human NSE ELISA kit (Alpha Diagnostic International Texas 78244, USA). Volume of infarct was measured by computed tomography (CT) scan using the preinstalled software Syngo (version A40A) of Siemen's medical solutions (Forchheim, Germany). Stroke severity at admission was assessed using Glasgow coma scale (GCS) and functional neurological outcome was assessed using modified Rankin scale (mRS) on day 30. Statistical analysis was performed using the SPSS software for windows version 15.0 (SPSS). A positive correlation was found between concentration of NSE on day 1 and infarct volume determined by CT scan (r = 0.955, P serve as a useful marker to predict stroke severity and early functional outcome. However, larger studies with serial estimation of NSE are needed to establish these observations more firmly.

  13. Combined Scintigraphy and Tumor Marker Analysis Predicts Unfavorable Histopathology of Neuroblastic Tumors with High Accuracy.

    Directory of Open Access Journals (Sweden)

    Wolfgang Peter Fendler

    Full Text Available Our aim was to improve the prediction of unfavorable histopathology (UH in neuroblastic tumors through combined imaging and biochemical parameters.123I-MIBG SPECT and MRI was performed before surgical resection or biopsy in 47 consecutive pediatric patients with neuroblastic tumor. Semi-quantitative tumor-to-liver count-rate ratio (TLCRR, MRI tumor size and margins, urine catecholamine and NSE blood levels of neuron specific enolase (NSE were recorded. Accuracy of single and combined variables for prediction of UH was tested by ROC analysis with Bonferroni correction.34 of 47 patients had UH based on the International Neuroblastoma Pathology Classification (INPC. TLCRR and serum NSE both predicted UH with moderate accuracy. Optimal cut-off for TLCRR was 2.0, resulting in 68% sensitivity and 100% specificity (AUC-ROC 0.86, p < 0.001. Optimal cut-off for NSE was 25.8 ng/ml, resulting in 74% sensitivity and 85% specificity (AUC-ROC 0.81, p = 0.001. Combination of TLCRR/NSE criteria reduced false negative findings from 11/9 to only five, with improved sensitivity and specificity of 85% (AUC-ROC 0.85, p < 0.001.Strong 123I-MIBG uptake and high serum level of NSE were each predictive of UH. Combined analysis of both parameters improved the prediction of UH in patients with neuroblastic tumor. MRI parameters and urine catecholamine levels did not predict UH.

  14. Improving and Evaluating Nested Sampling Algorithm for Marginal Likelihood Estimation

    Science.gov (United States)

    Ye, M.; Zeng, X.; Wu, J.; Wang, D.; Liu, J.

    2016-12-01

    With the growing impacts of climate change and human activities on the cycle of water resources, an increasing number of researches focus on the quantification of modeling uncertainty. Bayesian model averaging (BMA) provides a popular framework for quantifying conceptual model and parameter uncertainty. The ensemble prediction is generated by combining each plausible model's prediction, and each model is attached with a model weight which is determined by model's prior weight and marginal likelihood. Thus, the estimation of model's marginal likelihood is crucial for reliable and accurate BMA prediction. Nested sampling estimator (NSE) is a new proposed method for marginal likelihood estimation. The process of NSE is accomplished by searching the parameters' space from low likelihood area to high likelihood area gradually, and this evolution is finished iteratively via local sampling procedure. Thus, the efficiency of NSE is dominated by the strength of local sampling procedure. Currently, Metropolis-Hasting (M-H) algorithm is often used for local sampling. However, M-H is not an efficient sampling algorithm for high-dimensional or complicated parameter space. For improving the efficiency of NSE, it could be ideal to incorporate the robust and efficient sampling algorithm - DREAMzs into the local sampling of NSE. The comparison results demonstrated that the improved NSE could improve the efficiency of marginal likelihood estimation significantly. However, both improved and original NSEs suffer from heavy instability. In addition, the heavy computation cost of huge number of model executions is overcome by using an adaptive sparse grid surrogates.

  15. Significance of serum neuron-specific enolase in patients with acute traumatic brain injury

    Institute of Scientific and Technical Information of China (English)

    官卫; 杨伊林; 夏为民; 李璐; 龚德生

    2003-01-01

    Objective: To study the association between serum neuron-specific enolase (NSE) and the extent of brain damage and the outcome after acute traumatic brain injury (TBI). Methods: The release patterns of serum NSE in 78 patients after acute TBI were analyzed by using the enzyme linked immunosobent assay. The levels of NSE were compared with Glasgow coma scale, the category of brain injury and the outcome after 6 months of injury. Results: There were different NSE values in patients with minor (12.96 μg/L±2.39 μg/L), moderate (23.44 μg/L±5.33 μg/L) and severe brain injury (42.68 μg/L±4.57 μg/L). After severe TBI, the concentration of NSE in patients with epidural hematomas was 13.38 μg/L±4.01 μg/L, 24.03 μg/L±2.85 μg/L in brain contusion without surgical intervention group, 55.20 μg/L±6.35 μg/L in brain contusion with surgical intervention group, and 83.85 μg/L±15.82 μg/L in diffuse brain swelling group. There were close correlations between NSE values and Glasgow coma scale (r=-0.608, P<0.01) and the extent of brain injury (r=0.75, P<0.01). Patients with poor outcome had significantly higher initial and peak NSE values than those with good outcome (66.40 μg/L±9.46 μg/L, 94.24 μg/L±13.75 μg/L vs 32.16 μg/L±4.21 μg/L, 34.08 μg/L±4.40 μg/L, P<0.01, respectively). Initial NSE values were negatively related to the outcome (r=-0.501, P<0.01). Most patients with poor outcomes had persisting or secondary elevated NSE values. Conclusions: Serum NSE is one of the valuable neurobiochemical markers for assessment of the severity of brain injury and outcome prediction.

  16. Science.gov (United States)

    Olivares Cortes, Sonia; Araneda Flores, Jacqueline; Morales Illanes, Gladys; Leyton Dinamarca, Bárbara; Bustos Zapata, Nelly; Hernández Moreno, María Angélica; Oyarzún Macchiavello, María Teresa

    2017-03-30

    Introducción: el 27 de junio de 2016 se implementó en Chile la Ley sobre Composición Nutricional de los Alimentos y su Publicidad, que implica rotular el envase de los alimentos procesados, altos en calorías, grasas saturadas, azúcares y sodio.Objetivo: determinar las actitudes de escolares de 8 a 12 años, de distinto nivel socioeconómico (NSE) y estado nutricional, ante el nuevo etiquetado de los alimentos.Métodos: se aplicó una encuesta validada previamente, agregando preguntas sobre los nuevos sellos en los envases de alimentos y bebidas. Se realizó un análisis descriptivo de las variables estudiadas y se determinaron diferencias según NSE y estado nutricional con la prueba de Chi2.Resultados: no se observaron diferencias por género o ciudad. Al consultar sobre los nuevos sellos, el 87,3% de los niños de NSE medio-alto y 78,5% de NSE bajo señaló que les gustaba ser informados del contenido de los alimentos (p < 0,01). Dejarían de comprar los alimentos con sello el 53% de NSE medio-alto y 48% de NSE bajo. Del 14% al 22% seguirían comiendo galletas dulces, bebidas azucaradas, chocolates y papas fritas, sin diferencias por NSE. Los niños de estado nutricional normal y NSE medio-alto dieron mayor importancia a los sellos altos en calorías, grasas saturadas y sodio, y los de NSE bajo al contenido alto en azúcar. Entre los niños con sobrepeso y obesidad, los de NSEmedio-alto consideraron más importantes los 4 sellos que los de NSE bajo.Discusión: estos resultados facilitarán el apoyo educativo y publicitario para favorecer la comprensión y cumplimiento de la Ley.

  17. Evaluating the prognosis and degree of brain injury by combined S-100 protein and neuron specific enolase determination

    Institute of Scientific and Technical Information of China (English)

    Xihua Wang; Xinding Zhang

    2006-01-01

    Background:S-100 and neuron specific enolase(NSE)possess the characteristics of specific distribution in brain and relative stable content.Some studies suggest that combined detection of the both is of very importance for evaluating the degree of brain injury.OBJECTIVE: To observe the changes of S-100 protein and NSE levels at different time points after acute brain injury,and evaluate the values of combined detection detection of the both for different injury degrees,pathological changes and prognosis.DESIGN: Case-control observation SETTING: Department of Neurosurgery,Second Affiliated Hospital,Lanzhou University.PARTICIPANTS:Thirty-four inpatients with brain injury,19 males and 15 females,aged 15 to 73 years.who received treatment between September 2005 and May 2006 in the Department of Neurosurgery. Second Affiliated Hospital,Lanzhou University,were recruited.The patients were admitted to hospital at 24 hours after brain injury.After admission,skull CT confirmed that they suffered from brain injury.Following Glasgow coma score(GCS)on admission,the patients were assigned into 3 groups:severe group(GCS 3 to 8 points,n=15).moderate group(GCS 9 to 12 points,n=8)and mild group(GCS 13 to 15 points,n=11).Following Glasgow outcome scale(GOS)at 3 months after brain injury,the patients were assigned into good outcome group (GOS 4 to 5 points,good recovery and moderate disability included,n=19)and poor outcome group(GOS 1 to 3 points,severe disability,vegetative state and death,n=15).Ten subjects who received health examination concurrently were chosen as normal control group,including 6 males and 4 females,aged(45.4±14.3)years.In our laboratory,the normal level of NSE was≤15.2 ng/L,and that of S100 was≤0.105 μg/L.METHODS:①Blood samples of control group were collected when the subjects received health examination Blood samples of patients with brain injury were collected at 24 hours,3,7 and 14 days after injury.According to the instructions of NSE and S-100 kits

  18. 血清胃泌素释放肽前体在小细胞肺癌诊断和分期中的临床意义%The clinical significance of serum progastrin releasing peptide in the diagnosis and staging of small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    高振远; 欧毅梅; 张竞竞; 张浩然; 毕明宏

    2016-01-01

    Objective:To explore the clinical value of the levels of serum progastrin releasing peptide(proGRP)in the differential diagnosis and clinical staging of small cell lung cancer( SCLC) and non-small cell lung cancer( NSCLC) . Methods:The serum levels of proGRP in 62 patients with SCLC( including 25 cases of limited stage and 37 cases of extensive stage) and 38 patients with NSCLC were measured by chemiluminescent immunoassay,which was compared with neuron specific enolase( NSE) . The sensitivity,specificity and tumor staging in all patients were analyzed. Results:The sensitivities of the single detection of proGRP, NSE and combination detection of proGRP and NSE in diagnosing SCLC were 82. 93%, 64. 15% and 71. 58%, respectively. The positive rate of the combination detection of proGRP and NSE in diagnosing SCLC was significantly higher than that of the single detection of NSE, the difference of the positive rate between combination detection proGRP and NSE and single detection of proGRP was not statistically significant(P>0. 05). The serum levels of proGRP and NSE in patients with SCLC were obviously higher than those in NSCLC patients (P0.05)。 SCLC组患者血清proGRP和NSE水平均显著高于NSCLC 组(P<0.01)。 SCLC广泛期患者血清proGRP和NSE值均显著高于其局限期患者(P<0.01)。结论:proGRP作为SCLC的标志物,具有敏感度高的特点,并可反映SCLC的分期。 proGRP和NSE联合检测可提高SCLC诊断的准确率。

  19. 纳米硒对氟胁迫下鲤鱼肝抗氧化功能及组织结构的影响%Effects of nano-selenium on antioxidant capacity and histopathology of Cyprinus carpio liver under fluoride stress

    Institute of Scientific and Technical Information of China (English)

    陈剑杰; 曹谨玲; 罗永巨; 李巨银

    2013-01-01

    为探讨纳米硒(nano selenium,NSe)对氟暴露下鲤鱼肝脏的抗氧化功能和组织结构的保护作用,将750条鲤鱼随机分为5个组,分别为对照组(CK)、氟胁迫组(FS)和纳米硒低、中、高剂量组(NSe L、NSeM、NSeH),NSe L、NSeM、NSeH组在基础饵料中分别添加0.1、0.5、1.0 mg·kg-1的纳米硒,FS和NSe组用100 mg·L-1氟溶液进行胁迫,试验时间为30 d.结果表明:与CK组比较,氟暴露降低了鲤鱼肝组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性,增加了丙二醛(MDA)含量,肝脏的组织结构受到一定程度的损伤;与FS组比较,NSe增强了鲤鱼肝组织中SOD、CAT和GSH-Px活性,降低了MDA含量,缓解了氟胁迫对鲤鱼肝脏组织结构的损伤.表明饵料中添加纳米硒可在一定程度上改善氟胁迫所造成的鲤鱼抗氧化能力的下降和组织结构的损伤.

  20. No more tears? Maternal involvement during the newborn screening examination.

    LENUS (Irish Health Repository)

    Ganda, Augustine Josie

    2012-01-31

    BACKGROUND: Babies often show signs of discomfort and distress by crying during the neonatal screening examination (NSE). The authors hypothesized that supporting the baby with maternal participation may reduce infant crying during NSE. The objective of this study was to document incidental infant crying during NSE, before and after training residents, on maternal involvement and infant comfort techniques to help. METHODS: A total of 20 NSEs of normal newborn babies by pediatric residents were observed (video-recorded) following informed consent of the doctor and the baby\\'s mother. The examining doctors were then taught how to use maternal participation and developmental care (MPDC) comfort techniques to support the baby during NSE. Mothers were shown how to focus on their baby\\'s needs by supporting the baby\\'s head (preventing atonic neck reflexes) and, if necessary, providing nonnutritive sucking to the baby and an encouraging, repetitive low-tone voices to sooth the baby. A further 14 NSEs on different babies were video-recorded using these techniques. The video recordings were analyzed by independent observers for total length of crying and duration of crying during specific components of the NSE. Mothers in both groups were given a questionnaire to assess their opinions of the NSE. RESULTS: The median length of crying was significantly longer in the pre-MPDC group (93.5 seconds; range 0-198 seconds) compared with the post-MPDC infants (0 seconds; range 0-123 seconds; P = .001). Only 1 of 20 infants in the pre-MPDC did not cry during NSE compared with 8 of 14 babies in the post-MPDC group. CONCLUSION: Newborn infants cry less and mothers were more satisfied with NSEs when shown simple support and comfort techniques for their babies.

  1. Uncovering a Nuisance Influence of a Phenological Trait of Plants Using a Nonlinear Structural Equation: Application to Days to Heading and Culm Length in Asian Cultivated Rice (Oryza Sativa L.).

    Science.gov (United States)

    Onogi, Akio; Ideta, Osamu; Yoshioka, Takuma; Ebana, Kaworu; Yamasaki, Masanori; Iwata, Hiroyoshi

    2016-01-01

    Phenological traits of plants, such as flowering time, are linked to growth phase transition. Thus, phenological traits often influence other traits through the modification of the duration of growth period. This influence is a nuisance in plant breeding because it hampers genetic evaluation of the influenced traits. Genetic effects on the influenced traits have two components, one that directly affects the traits and one that indirectly affects the traits via the phenological trait. These cannot be distinguished by phenotypic evaluation and ordinary linear regression models. Consequently, if a phenological trait is modified by introgression or editing of the responsible genes, the phenotypes of the influenced traits can change unexpectedly. To uncover the influence of the phenological trait and evaluate the direct genetic effects on the influenced traits, we developed a nonlinear structural equation (NSE) incorporating a nonlinear influence of the phenological trait. We applied the NSE to real data for cultivated rice (Oryza sativa L.): days to heading (DH) as a phenological trait and culm length (CL) as the influenced trait. This showed that CL of the cultivars that showed extremely early heading was shortened by the strong influence of DH. In a simulation study, it was shown that the NSE was able to infer the nonlinear influence and direct genetic effects with reasonable accuracy. However, the NSE failed to infer the linear influence in this study. When no influence was simulated, an ordinary bi-trait linear model (OLM) tended to infer the genetic effects more accurately. In such cases, however, by comparing the NSE and OLM using an information criterion, we could assess whether the nonlinear assumption of the NSE was appropriate for the data analyzed. This study demonstrates the usefulness of the NSE in revealing the phenotypic influence of phenological traits.

  2. Application of serum NY-ESO-1 antibody assay for early SCLC diagnosis.

    Science.gov (United States)

    Yang, Jihua; Jiao, Shunchang; Kang, Jingbo; Li, Rong; Zhang, Guanzhong

    2015-01-01

    NY-ESO-1 antibody is one of the cancer-related antibodies. The purpose of this study was to investigate the diagnostic role of the NY-ESO-1 humoral immune response in small cell lung cancer (SCLC). We recombined the recombinant protein of NY-ESO-1 antibody and NSE, analyzed them by Enzyme-linked immunosorbent assay, and then established the Receiver Operating Characteristic (ROC) curve to estimate the diagnostic value of NY-ESO-1 antibody, NSE and their combinations. According to detection, the positive rate of NY-ESO-1 humoral immune response (26.3%), NSE (43.8%) and their combinations (10.5%) were all lower than the negative rate which indicated that the NY-ESO-1 antibody might be down-regulated in SCLC. And the positive rate wasn't related to clinicopathologic characteristics. The ROC curve demonstrated that with a 37.17% sensitivity and a 91.7% specificity along with a AUC of 0.619 for NY-ESO-1ab as well as with a 48.3% sensitivity and a 90.87% specificity along with a AUC value of 0.773 for NSE, their diagnostic value were both high. Besides, the diagnostic value of their combinations was also good for a AUC of 0.83 and a 69.12% sensitivity and a 91.8% specificity. There were significant difference of diagnostic value among three types above (NY-ESO-1 vs. NSE, P ESO-1, P ESO-1ab, NSE and their combinations all were important diagnostic markers for SCLC. Moreover, the diagnostic value of their combinations was higher than any single of them. And NY-ESO-1 humoral immune to NSE might be a potential diagnostic indicator in SCLC.

  3. Serum neuron specific enolase - a novel indicator for neuropsychiatric systemic lupus erythematosus?

    Science.gov (United States)

    Hawro, T; Bogucki, A; Krupińska-Kun, M; Maurer, M; Woźniacka, A

    2015-12-01

    Neuropsychiatric (NP) lupus, a common manifestation of systemic lupus erythematosus (SLE), is still insufficiently understood, in part, because of the lack of specific biomarkers. Neuron specific enolase (NSE), an important neuronal glycolytic enzyme, shows increased serum levels following acute brain injury, and decreased serum levels in several chronic disorders of the nervous system, including multi infarct dementia, multiple sclerosis and depression. The aim of the study was to evaluate serum NSE levels in SLE patients with and without nervous system involvement, and in healthy controls, and to assess the correlation of NSE serum levels of patients with neuropsychiatric systemic lupus erythematosus (NPSLE) with clinical parameters. The study comprised 47 SLE patients and 28 controls. SLE activity was assessed using the Systemic Lupus Activity Measure (SLAM). A neurologist and a psychiatrist examined all patients. NP involvement was diagnosed according to strict NPSLE criteria proposed by Ainiala and coworkers, as modification to American College of Rheumatology (ACR) nomenclature and case definitions. NSE serum levels were determined by use of an immunoassay. Mean NSE serum concentrations in patients with NPSLE were significantly lower than in non-NPSLE patients (6.3 ± 2.6 µg/L vs. 9.7 ± 3.3 µg/L, p < 0.01) and in controls (8.8 ± 3.3 µg/L, p < 0.05). There were significant negative correlations between NSE serum levels and SLE activity (r = -0.42, p < 0.05) and the number of NPSLE manifestations diagnosed (-0.37; p = 0.001). Decreased serum concentrations of NSE may reflect chronic neuronal damage with declined metabolism of the nervous tissue in patients with NPSLE. © The Author(s) 2015.

  4. Up-regulation of VEGF-C secreted by cancer cells and not VEGF-A correlates with clinical evaluation of lymph node metastasis in esophageal squamous cell carcinoma (ESCC).

    Science.gov (United States)

    Krzystek-Korpacka, Malgorzata; Matusiewicz, Malgorzata; Diakowska, Dorota; Grabowski, Krzysztof; Blachut, Katarzyna; Banas, Teresa

    2007-05-01

    Tissue expression of VEGF-C correlates with lymph node involvement (LNI) in ESCC and serum VEGF-C (sVEGF-C) in a non-small cell lung cancer has been more accurate marker of LNI than chest CT. Despite LNI importance in ESCC, the usefulness of serum VEGF-C (sVEGF-C) as a disease and LNI marker in ESCC has not been investigated yet. We found elevated sVEGF-C in ESCC (17.40 vs. 10.57 ng/ml in controls, pmarker than described elsewhere: CEA, CA19-9 and SCC-Ag, with: sensitivity--70%, specificity--81%, accuracy--83.7%. Analysis of sVEGF-C correlation with clinico-pathological cancer features revealed relation to LNI (N0: 15.77 vs. N1: 21.78 ng/ml, p=0.02), especially in advanced cancers. Serum VEGF-C as a marker of LNI was characterized by: sensitivity--76%, specificity--58%, accuracy--64.4%. No relation was observed between LNI and sVEGF-A or sVEGF-A/platelets (PLT). Because sVEGF-C was higher in N0 cancers (ptumor presence also up-regulates sVEGF-C. We found sVEGF-C correlation with PLT and WBC: R=0.36 and R=0.32 (pcancer features implies that elevation of sVEGF-C in N1 cancers is not related to them.

  5. Simultaneous electrochemical immunosensor based on water-soluble polythiophene derivative and functionalized magnetic material.

    Science.gov (United States)

    Zhang, Xiaoyue; Ren, Xiang; Cao, Wei; Li, Yueyun; Du, Bin; Wei, Qin

    2014-10-03

    A novel, sensitive electrochemical immunosensor for simultaneous determination of squamous cell carcinoma associated antigen (SCC-Ag) and carcinoembryonic antigen (CEA) for the combined diagnosis of cervical cancer was designed. The amplification strategy for electrochemical immunoassay was based on poly[3-(1,1'-dimethyl-4-piperidine-methylene) thiophene-2,5-diylchloride] (PDPMT-Cl) and functionalized mesoporous ferroferric oxide nanoparticles (Fe3O4 NPs). PDPMT-Cl dispersed in chitosan solution with enhanced electrical conductivity and solubility was used as matrices to immobilize the first antibodies. Different redox probes (thionine (Th) and ferrocenecarboxylic acid (Fca)) functionalized Fe3O4 NPs incubated with two kinds of secondary antibodies to fabricate the labels. Using an electrochemical analysis technique, two well-separated peaks were generated by Th and Fca, making the simultaneous detection of two analytes on the electrode possible. Under optimized conditions, this method showed wide linear ranges of three orders of magnitude with the detection limits of 4 pg mL(-1) and 5 pg mL(-1), respectively. The disposable immunosensor possessed excellent clinical value in cervical cancer screening as well as convenient point-of-care diagnostics.

  6. 应用EP-7A2文件评价三酰甘油对神经元特异性烯醇化酶的干扰%Evaluation the Interference of Neuron-Specific Enolase by Triglyceride Using EP-7A2 Files

    Institute of Scientific and Technical Information of China (English)

    黄惠; 李强; 欧财文; 王志银

    2011-01-01

    Objective To explore the different concentrations of triglyceride (TG) interfere neuron-specific enolase (NSE)assayed by ELISA method. Methods According to CLSI EP-7A2 file,using electrochemical luminescence as the comparative method and ELISA method as the evaluated method for NSE assay. ①For NSE deviation (evaluated method-comparative method) as the Y axis,the concentration of the comparative method as the X-axis. 20 test samples and 20 samples were drawn the deviation map,comparative analysis of TG concentration between 1 and 4 mmol/L was detected by the interference of NSE (concentration between 5 and 20 ng/ml). ②Using TG concentration as the X-axis,the decresing value of NSE as Y-axis drawing the dose response curves of TG interfering NSE assay. Results ①TG concentration at 1~4 mmol/L has negative interference effect on NSE (concentration between 5 and 20 mmol/L) assayed by ELISA.② The dose response curves of TG interfering NSE showed that the TG concentration increasing accompanied by negative interference increased when the concentration of NSE at 5 ng/ml and 20 ng/ml. The dose-response curve was linear regression and equation were as Y= - 2. 4X+18. 92 and Y= - 0. 69X+5.26. Conclusion Using patient sera with high TG concentration for preparation of interference sera when the TG concentration between 1 mmol/L and 4 mmol/L,the assay of NSE has negative interference by ELISA,the interference dose curve were linear correlation.%目的 探讨不同浓度的三酰甘油(triglyceride,TG)对ELISA法检测神经元特异性烯醇化酶(neuron-specific enolase,NSE)的干扰.方法 依据CLSI EP-7A2文件,以电化学发光法为NSE检测的比较方法,ELISA法为NSE检测的被评价方法.①以NSE的偏差(被评价方法-比较方法)为Y轴,比较方法的NSE浓度为X轴,将20个测试标本和20个对照标本绘制偏差图,分析浓度为4 mmol/L的TG对比较方法检测NSE(浓度范围在5~20 ng/ml)的干扰.②再以TG浓度为X轴,其对应

  7. On the significance of the Nash-Sutcliffe efficiency measure for event-based flood models

    Science.gov (United States)

    Moussa, Roger

    2010-05-01

    When modelling flood events, the important challenge that awaits the modeller is first to choose a rainfall-runoff model, then to calibrate a set of parameters that can accurately simulate a number of flood events and related hydrograph shapes, and finally to evaluate the model performance separately on each event using multi-criteria functions. This study analyses the significance of the Nash-Sutcliffe efficiency (NSE) and proposes a new method to assess the performance of flood event models (see Moussa, 2010, "When monstrosity can be beautiful while normality can be ugly : assessing the performance of event-based-flood-models", Hydrological Science Journal, in press). We focus on the specific cases of events difficult to model and characterized by low NSE values, which we call "monsters". The properties of the NSE were analysed as a function of the calculated hydrograph shape and of the benchmark reference model. As application case, a multi-criteria analysis method to assess the model performance on each event is proposed and applied on the Gardon d'Anduze catchment. This paper discusses first the significance of the well-known Nash-Sutcliffe efficiency (NSE) criteria function when calculated separately on flood events. The NSE is a convenient and normalized measure of model performance, but does not provide a reliable basis for comparing the results of different case studies. We show that simulated hydrographs with low or negative values of NSE, called "monsters", can be due solely to a simple lag translation or a homothetic ratio of the observed hydrograph which reproduces the dynamic of the hydrograph, with acceptable errors on other criteria. In the opposite, results show that simulations with a NSE close to 1 can become "monsters" and give very low values (even negative) of the criteria function G, if the average observed discharged used as a benchmark reference model in the NSE is modified. This paper argues that the definition of an appropriate benchmark

  8. Clinical Significance of Detection of Serum Values of Neuron Specific Enolase Before and After Treatment for Small Cell Lung Cancer%小细胞肺癌患者治疗前后血清NSE测定的临床意义

    Institute of Scientific and Technical Information of China (English)

    薛峰; 王莉彦; 张明岩; 蔡莉

    2011-01-01

    Background and objective It has been proven that neuron specific enolase (NSE) was one of the sensitive tumor markers for small cell lung cancer (SCLC). The aim of this study is to investigate the usefulness of serum NSE values as diagnostic and prognostic factors in patients with SCLC. Methods Serum NSE was detected by electrochemiluminescence method In 57 SCLC cases pre- and post treatment. Survival curves were constructed using the Kaplan-Meier method and then compared using the Log-rank test. Results Pre-treatment serum NSE values are prognostic factors for overall survival of patients with SCLC. There was a statistically significant difference In the pretreatment NSE levels of patients who were at an extensive stage compared with the values of those patients who were at a limited stage (P<0.00l). The rate of change between the pre- and post-treatment serum NSE levels was not related to the overall survival (P=0.084). Conclusion NSE Is an Ideal tumor marker for the diagnosis and assessment prognosis of SCLC.%背景与目的,神经烯醇化酶(neuron specific enolase,NSE)是小细胞肺癌(small cell lung cancer,SCLC)敏感的肿瘤标记物之一.本研究旨在研究血清NSE水平作为SCLC诊断和预后因索的实用性.方法,治疗前后应用电化学发光法测定57例SCLC患者的血清NSE.应用Kaplan-Meier法构建生存曲线,采用对数秩检验法(Log-rank)进行曲线之间的比较.结果,SCLC患者治疗前血清NSE水平是患者总生存期的预后因素,广泛期患者与局限期患者的治疗前血清NSE水平有统计学差异(P<0.001),治疗前后NSE的变化率与总生存期无关(P=0.084).结论,NSE是SCLC患者诊断和评估预后的理想肿瘤标记物.

  9. Elevated neurofilament light chain (NFL) mRNA levels in prediabetic peripheral neuropathy.

    Science.gov (United States)

    Celikbilek, Asuman; Tanik, Nermin; Sabah, Seda; Borekci, Elif; Akyol, Lutfi; Ak, Hakan; Adam, Mehmet; Suher, Murat; Yilmaz, Neziha

    2014-06-01

    Evidence suggests that peripheral nerve injury occurs during the early stages of disease with mild glycemic dysregulation. Two proteins, neuron-specific enolase (NSE) and neurofilament light chain (NFL), have been examined previously as possible markers of neuronal damage in the pathophysiology of neuropathies. Herein, we aimed to determine the potential value of circulatory NSE and NFL mRNA levels in prediabetic patients and in those with peripheral neuropathy. This prospective clinical study included 45 prediabetic patients and 30 age- and sex-matched controls. All prediabetic patients were assessed with respect to diabetes-related microvascular complications, such as peripheral neuropathy, retinopathy and nephropathy. mRNA levels of NSE and NFL were determined in the blood by real-time polymerase chain reaction. NSE mRNA levels were similar between prediabetic and control groups (p > 0.05), whereas NFL mRNA levels were significantly higher in prediabetics than in controls (p 0.05), while NFL mRNA levels were significantly higher in prediabetics with peripheral neuropathy than in those without (p = 0.038). According to correlation analysis, NFL mRNA levels were positively correlated with the Douleur Neuropathique 4 questionnaire score in prediabetic patients (r = 0.302, p = 0.044). This is the first study to suggest blood NFL mRNA as a surrogate marker for early prediction of prediabetic peripheral neuropathy, while NSE mRNA levels may be of no diagnostic value in prediabetic patients.

  10. The effect of N-stearoylethanolamine on plasma lipid composition in rats with experimental insulin resistance

    Directory of Open Access Journals (Sweden)

    O. V. Onopchenko

    2015-02-01

    Full Text Available A model of insulin resistance (IR, induced by prolonged high fat diet with high content of saturated fats was used to investigate the effect of N-stearoylethanolamine (NSE on the composition of free fatty acids (FFA, plasma lipoprotein spectrum and content of proinflammatory cytokine TNFα in rats. The results of this work showed a rise in the content of monounsaturated fatty acids (18:1 n-9 and a reduction in the level of polyunsaturated fatty acids (20:4 n-6 in plasma of rats with experimental IR. These findings are accompanied by the increased TNFα production and significant changes in plasma lipoprotein profile of rats with the fat overload. Particularly, a decreased high-density lipoprotein (HDL cholesterol level and increased low-density (LDL and very low-density lipoprotein (VLDL cholesterol level were detected. The NSE administration to obese rats with IR restored the content of mono- and polyunsaturated FFA, increased HDL cholesterol content and reduced LDL cholesterol level. In addition, the IR rats treated with NSE showed normalization in the serum TNFα level. Our results showed the restoration of plasma lipid profile under NSE administration in rats with obesity-induced IR. Considering the fact that plasma lipid composition displays the lipid metabolism in general, the NSE actions may play a significant role in the prevention of IR-associated complications.

  11. Comparison of Different Nanosuspensions as Potential Ophthalmic Delivery Systems for Ketotifen Fumarate

    Science.gov (United States)

    Soltani, Saieede; Zakeri-Milani, Parvin; Barzegar-Jalali, Mohammad; Jelvehgari, Mitra

    2016-01-01

    Purpose: The objective of this study was to develop, characterize, and comparatively investigate the ketotifen fumarate (KF) nanosuspensions (NSS) to enhance the permeability of KF. Methods: In the present work, the NSP and NSE were prepared by double-emulsion solvent evaporation/nanoprecipitation methods with poly (D,Llactide-co-glycolide) and Eudragit RL100 polymers, respectively. The loading efficiency, particle size, and polydispersity index of prepared different NSs were evaluated with scanning electron microscopy (SEM), X-ray diffraction, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and in vitro release and transcorneal permeation . NSs were also compared on the basis of particle size and polydispersity index. Results: Particle size, polydispersity index, and loading efficiency of NSP1 and NSE3 showed the best value (158 nm, 117 nm, 0.21, 0.43 and 43%, 95.23%, respectively). SEM showed spherical globules and DSC results showed the reduction in crystallinity. The NSE3 formulations demonstrated significantly (p<0.05) higher drug release rates than the NSP1 due to increases in the surface area. Comparative studies showed that NSE release and permeability are higher than NSP. Conclusion: It is concluded that both NSP and NSE provide a useful dosage form for the ocular drug delivery which can enhance the permeability of KF. PMID:27766218

  12. Neutronization and Energetics of Type Ia Supernovae

    Science.gov (United States)

    Seitenzahl, I. R.; Peng, F.; Townsley, D. M.; Calder, A. C.

    2005-12-01

    Type Ia supernovae are critical distance indicators for cosmology. The lightcurves are powered by the decay of radioactive nickel and cobalt isotopes. The amount of nickel produced in the supernova event depends on the detailed trajectories of the hydrodynamic evolution of the explosion. A key ingredient in numerical simulations of the deflagration phase of Type Ia supernovae is the nuclear flame model. A realistic model must accurately describe the nuclear energy released, the timescale on which the energy release occurs, and the changes in composition that constitute the burning. Once the flame has passed, the hot products of the burning constitute a nuclear statistical equilibrium (NSE) abundance distribution. Since the NSE abundances, and hence derived quantities such as the mean binding energy per nucleon, are functions of the density, temperature and electron fraction, it is important to continuously adjust the NSE state of the ashes during the hydrodynamic evolution of the star. Weak interactions influence the energetics and evolution via the change in degeneracy pressure due to captured electrons, the energy losses carried away by neutrinos, and the readjustment of the NSE state following a change in the electron fraction. We have developed a NSE-based model, which implements these features for a hydrodynamical evolution code.

  13. Development of an algorithm for phenotypic screening of carbapenemase-producing Enterobacteriaceae in the routine laboratory.

    Science.gov (United States)

    Robert, Jérôme; Pantel, Alix; Merens, Audrey; Meiller, Elodie; Lavigne, Jean-Philippe; Nicolas-Chanoine, Marie-Hélène

    2017-01-17

    Carbapenemase-producing Enterobacteriaceae (CPE) are difficult to identify among carbapenem non-susceptible Enterobacteriaceae (NSE). We designed phenotypic strategies giving priority to high sensitivity for screening putative CPE before further testing. Presence of carbapenemase-encoding genes in ertapenem NSE (MIC > 0.5 mg/l) consecutively isolated in 80 French laboratories between November 2011 and April 2012 was determined by the Check-MDR-CT103 array method. Using the Mueller-Hinton (MH) disk diffusion method, clinical diameter breakpoints of carbapenems other than ertapenem, piperazicillin+tazobactam, ticarcillin+clavulanate and cefepime as well as diameter cut-offs for these antibiotics and temocillin were evaluated alone or combined to determine their performances (sensitivity, specificity, positive and negative likelihood ratios) for identifying putative CPE among these ertapenem-NSE isolates. To increase the screening specificity, these antibiotics were also tested on cloxacillin-containing MH when carbapenem NSE isolates belonged to species producing chromosomal cephalosporinase (AmpC) but Escherichia coli. Out of the 349 ertapenem NSE, 52 (14.9%) were CPE, including 39 producing OXA-48 group carbapenemase, eight KPC and five MBL. A screening strategy based on the following diameter cut offs, ticarcillin+clavulanate carbapenemase production before further CPE confirmatory testing.

  14. Nasal and oral snoring endoscopy: novel and promising diagnostic tools in OSAS patients.

    Science.gov (United States)

    Lovato, Andrea; Kotecha, Bhik; Vianello, Andrea; Giacomelli, Luciano; Staffieri, Alberto; Marchese-Ragona, Rosario

    2015-07-01

    The aim of the present study was to investigate if any of the three awake procedures [fiberoptic nasopharyngoscopy with modified Müller Maneuver (FNMM), nasal snoring endoscopy (NSE), or oral snoring endoscopy (OSE)] could efficiently predict the grade or pattern of upper airway (UA) collapse found with drug-induced sleep endoscopy (DISE), which is considered by many authors as the current gold standard in optimizing obstructive sleep apnea syndrome (OSAS) patient selection for UA surgery. Twenty consecutive patients (simple snorers and OSAS patients) were studied with FNMM, NSE, OSE, and DISE. The inter-test agreement was evaluated with Cohen's kappa coefficient (κ). In the current series, we found that NSE and OSE were better than FNMM in predicting the pattern of collapse found with DISE. A significant pattern agreement between NSE and DISE was present in all sub-sites, and the agreement was measured with a scale proposed by Landis and Koch as: moderate in velo- and oropharynx (κ = 0.52, p = 0.001, and κ = 0.47, p = 0.003, respectively), and substantial in hypopharynx (κ = 0.63, p DISE, the pattern agreement was almost perfect at oropharyngeal level (κ = 0.82, p DISE only at oropharyngeal level (κ = 0.31, p = 0.009); while in the other sub-sites, no significant agreement was found. NSE and OSE are new promising diagnostic tools in OSAS patients. Further investigations are needed to see if they could predict the effectiveness of UA surgery.

  15. A wireless point-of-care testing system for the detection of neuron-specific enolase with microfluidic paper-based analytical devices.

    Science.gov (United States)

    Fan, Yan; Liu, Juntao; Wang, Yang; Luo, Jinping; Xu, Huiren; Xu, Shengwei; Cai, Xinxia

    2017-09-15

    Neuron-specific enolase (NSE) had clinical significance on diagnosis, staging, monitoring effect and judging prognosis of small cell lung cancer. Thus, there had a growing demand for the on-site testing of NSE. Here, a wireless point-of-care testing (POCT) system with electrochemical measurement for NSE detection was developed and verified. The wireless POCT system consisted of microfluidic paper-based analytical devices (μPADs), electrochemical detector and Android's smartphone. Differential pulse voltammetry (DPV) measurement was adopted by means of electrochemical detector which including a potentiostat and current-to-voltage converter. μPADs were modified with nanocomposites synthesized by Amino functional graphene, thionine and gold nanoparticles (NH2-G/Thi/AuNPs) as immunosensors for NSE detection. Combined with μPADs, the performance of the wireless POCT system was evaluated. The peak currents showed good linear relationship of the logarithm of NSE concentration ranging from 1 to 500ngmL(-1) with the limit of detection (LOD) of 10pgmL(-1). The detection results were automatically stored in EEPROM memory and could be displayed on Android's smartphone through Bluetooth in real time. The detection results were comparable to those measured by a commercial electrochemical workstation. The wireless POCT system had the potential for on-site testing of other tumor markers. Copyright © 2017 Elsevier B.V. All rights reserved.

  16. Comparison of Different Nanosuspensions as Potential Ophthalmic Delivery Systems for Ketotifen Fumarate.

    Science.gov (United States)

    Soltani, Saieede; Zakeri-Milani, Parvin; Barzegar-Jalali, Mohammad; Jelvehgari, Mitra

    2016-09-01

    Purpose: The objective of this study was to develop, characterize, and comparatively investigate the ketotifen fumarate (KF) nanosuspensions (NSS) to enhance the permeability of KF. Methods: In the present work, the NSP and NSE were prepared by double-emulsion solvent evaporation/nanoprecipitation methods with poly (D,Llactide-co-glycolide) and Eudragit RL100 polymers, respectively. The loading efficiency, particle size, and polydispersity index of prepared different NSs were evaluated with scanning electron microscopy (SEM), X-ray diffraction, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and in vitro release and transcorneal permeation . NSs were also compared on the basis of particle size and polydispersity index. Results: Particle size, polydispersity index, and loading efficiency of NSP1 and NSE3 showed the best value (158 nm, 117 nm, 0.21, 0.43 and 43%, 95.23%, respectively). SEM showed spherical globules and DSC results showed the reduction in crystallinity. The NSE3 formulations demonstrated significantly (p<0.05) higher drug release rates than the NSP1 due to increases in the surface area. Comparative studies showed that NSE release and permeability are higher than NSP. Conclusion: It is concluded that both NSP and NSE provide a useful dosage form for the ocular drug delivery which can enhance the permeability of KF.

  17. 急性大面积脑梗死患者血清神经元特异性烯醇化酶、S100蛋白含量的变化

    Institute of Scientific and Technical Information of China (English)

    曲艳霞

    2012-01-01

    目的 探讨检测血清中神经元特异性烯醇化酶(NSE)和S100 蛋白变化对急性大面积脑梗死患者预后的临床意义.方法 大面积脑梗死患者发病7d 内检测血清NSE 水平和S100 蛋白浓度,并与腔隙性脑梗死患者进行比较.结果 大面积脑梗死组患者急性期血清NSE水平和S100 蛋白变化较腔隙性脑梗死组显著升高(P<0.05);有意识障碍组与无意识障碍组、存活组与死亡组血清NSE 水平和S100 蛋白变化有显著差异(P<0.05).结论 大面积脑梗死患者急性期血清NSE 水平和S100 蛋白变化与病情严重程度一致;检测血清NSE 水平和S100 蛋白变化有助于评估病情严重程度及预后判断.

  18. Essential Strategies for Revealing Nanoscale Protein Dynamics by Neutron Spin Echo Spectroscopy.

    Science.gov (United States)

    Callaway, David J E; Bu, Zimei

    2016-01-01

    Determining the internal motions of a protein on nanosecond-to-microsecond timescales and on nanometer length scales is challenging by experimental biophysical techniques. Neutron spin echo spectroscopy (NSE) offers a unique opportunity to determine such nanoscale protein domain motions. However, the major hurdle in applying NSE to determine nanoscale protein motion is that the time and length scales of internal protein motions tend to be comparable to that of the global motions of a protein. The signals detected by NSE tend to be dominated by rigid-body translational and rotational diffusion. Using theoretical analyses, our laboratory showed that selective deuteration of a protein domain or a subunit can enhance the capability of NSE to reveal the internal motions in a protein complex. Here, we discuss the essential theoretical analysis and experimental methodology in detail. Protein nanomachines are far more complex than any molecular motors that have been artificially constructed, and their skillful utilization likely represents the future of medicine. With selective deuteration, NSE will allow us to see these nanomachines in motion. © 2016 Elsevier Inc. All rights reserved.

  19. [Validity of indicators on physical activity and sedentary behavior from the Brazilian National School-Based Health Survey among adolescents in Rio de Janeiro, Brazil].

    Science.gov (United States)

    Tavares, Letícia Ferreira; Castro, Inês Rugani Ribeiro de; Cardoso, Letícia Oliveira; Levy, Renata Bertazzi; Claro, Rafael Moreira; Oliveira, Andreia Ferreira de

    2014-09-01

    This study evaluated the relative validity of physical activity indicators from the questionnaire used in the Brazilian National School-Based Health Survey (PeNSE) in the city of Rio de Janeiro, Brazil, based on a sample of 174 students. The following indicators of weekly physical activity were evaluated: ACTIVE-300MIN (≥ 300 minutes/week); ACTIVE-150MIN (≥ 150 minutes), INACTIVE (no physical activity). Additionally, indicators of sedentary behavior were also assessed, as daily screen time (TV, videogames, and computer). The results from the questionnaire were compared with three 24-hour recalls. The results of ACTIVE-300MIN, ACTIVE-150MIN, and INACTIVE generated by PeNSE showed high accuracy. These indicators performed better than those of sedentary behavior in relation to frequency estimates as well as sensitivity, specificity, and correct classification rate. The indicators of physical activity from PeNSE showed satisfactory relative validity.

  20. On the Estimation of Carrier Frequency Offset and DC Offset for OFDM Systems

    Science.gov (United States)

    Lin, Hai; Nakao, Takeshi; Lu, Weiming; Yamashita, Katsumi

    In an orthogonal frequency division multiplexing (OFDM) receiver with direct-conversion architecture, carrier frequency offset (CFO) and direct-current offset (DCO), which cause severe performance degradation, need to be estimated and compensated. Recently, by investigating the subspace of OFDM signal after coarse DCO cancellation using timedomain average, we have proposed a nullspace-based estimator (NSE), for blind CFO and DCO estimation. In this paper, based on an analysis of the cost function of the NSE, we propose a common nullspace based estimator (CNSE). It is shown that by matching the frequency occupation of the received OFDM signal with CFO and DCO, the CNSE can achieve the full performance potential of the NSE. Also, the performance analysis reveals that the CNSE can asymptotically approach the Cramer-Rao bound (CRB) of OFDM CFO estimation in the presence of DCO. Finally the analysis results are confirmed by simulations.

  1. Early detection of response in small cell bronchogenic carcinoma by changes in serum concentrations of creatine kinase, neuron specific enolase, calcitonin, ACTH, serotonin and gastrin releasing peptide

    DEFF Research Database (Denmark)

    Bork, E; Hansen, M; Urdal, P;

    1988-01-01

    determined within 4-8 weeks. The results indicate that serum CK-BB and NSE are potential markers for SCC at the time of diagnosis and that changes in the concentrations during the first course of cytostatic therapy are promising as biochemical tests for early detection of response to chemotherapy.......Creatine kinase (CK-BB), neuron specific enolase (NSE), ACTH, calcitonin, serotonin and gastrin releasing peptide (GRP) were measured in serum or plasma before and immediately after initiation of treatment in patients with small cell lung cancer (SCC). Pretherapeutic elevated concentrations of CK...... stage patients and 71% in limited stage patients. Frequent initial monitoring of the substances showed an increase in the concentrations of pretherapeutic elevated CK-BB and NSE on day 1 or 2 followed by a sharp decrease within 1 week. These changes were correlated to objective clinical response...

  2. Neuron specific enolase demonstration in the diagnosis of a solid-cystic (papillary cystic) tumour of the pancreas.

    Science.gov (United States)

    Chott, A; Klöppel, G; Buxbaum, P; Heitz, P U

    1987-01-01

    Immunoreactivity to neuron specific enolase (NSE) was demonstrated in a solid-cystic (papillary cystic) tumour of the human pancreas, employing immunohistochemical methods. Positive staining for NSE was found with two different antisera. In addition, sodium-dodecyl-sulphate-polyacrylamide-gel-electro-phoresis (SDS-PAGE) of tumour homogenate revealed a distinct band reacting with a NSE antiserum. However, we failed to detect any hormonal products or neuroendocrine granules in the tumour. Therefore the authors advise caution in using the enzyme as a differential diagnostic tool, especially in surgical pathology of epithelial pancreatic neoplasms occurring in young females. In individual cases electron microscopy will be necessary since solid-cystic tumours of the pancreas consistently show large intracytoplasmic zymogen-like granules.

  3. Fractal Analysis of Prime Indian STOCK Market Indices

    Science.gov (United States)

    Samadder, Swetadri; Ghosh, Koushik; Basu, Tapasendra

    2013-03-01

    The purpose of the present work is to study the fractal behaviour of prime Indian stock exchanges, namely Bombay Stock Exchange Sensitivity Index (BSE Sensex) and National Stock Exchange (NSE). To analyze the monofractality of these indices we have used Higuchi method and Katz method separately. By applying Mutifractal Detrended Fluctuation Analysis (MFDFA) technique we have calculated the generalized Hurst exponents, multifractal scaling exponents and generalized multifractal dimensions for the present indices. We have deduced Hölder exponents as well as singularity spectra for BSE and NSE. It has been observed that both the stock exchanges are possessing self-similarity at different small ranges separately and inhomogeneously. By comparing the multifractal behaviour of the BSE and NSE indices, we have found that the second one exhibits a richer multifractal feature than the first one.

  4. Concepciones de tweens chilenos respecto a la publicidad emitida por televisión

    Directory of Open Access Journals (Sweden)

    Carlos del Valle

    2010-01-01

    Full Text Available Esta investigación buscó identificar los usos y significados que escolares de 11 a 12 años de edad (tweens otorgan a la publicidad emitida por televisión. En la etapa cuantitativa se aplicó el 'Cuestionario de hábitos de consumo televisivo y prácticas de uso del dinero' a una muestra de 137 escolares, de los cuales 28 pertenecen al nse alto, 62 al nse medio-alto y 47 al nse bajo. En la etapa cualitativa se aplicó una entrevista en profundidad a 30 escolares de la muestra. Los resultados señalan la existencia de diferencias significativas según género y grado socioeconómico, lo cual permite hipotetizar que estas variables poseen influencia en los usos, opiniones y percepciones que los tweens poseen de la publicidad.

  5. Projection of the rotation form Navier-Stokes equation onto the half-staggered grid

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Ji Ryong [Inje University, Kimhae (Korea, Republic of)

    2016-07-15

    A projection method for computing incompressible fluid flow is proposed. For the method, the rotation form Navier-Stokes equation (NSE), for which the velocity and the total pressure are employed, is discretized on the half-staggered, finite difference spatial grid. The total pressure couples the static pressure gradient and the convection of momentum in the continuous NSE while the half-staggered grid provides weak pressure-velocity coupling in discrete space. These two features interact synergistically for the discretized NSE to produce smooth pressure fields without additional numerical artifacts such as the momentum interpolation. The method preserves the kinetic energy at the inviscid limit condition. Numerical solutions of the decaying Taylor vortex, the inviscid Taylor vortex, the sudden expansion channel and the square-prism wake are presented.

  6. The effect of amphiphilic polymers with a continuous amphiphilicity profile on the membrane properties in a bicontinuous microemulsions studied by neutron scattering

    Science.gov (United States)

    Klemmer, Helge F. M.; Frielinghaus, Henrich; Allgaier, Jürgen; Ohl, Michael; Holderer, Olaf

    2017-06-01

    Microemulsion systems consisting of oil, water and surfactant have been studied with neutron scattering techniques. The amount of surfactant needed to form a microemulsion can be dramatically reduced by the addition of small amounts of amphiphilic block copolymers (boosting effect). Here, we studied the influence of block copolymers with gradually changing amphiphilicity from hydrophilic to hydrophobic. Small angle neutron scattering (SANS), neutron spin echo spectroscopy (NSE) and phase diagram measurements in combination give access to the elastic properties of the membrane. The underlying NSE experiments for this interpretation rely on smallest changes of the relaxation curves (of ca. 1% steps) for still small changes of the bending rigidity (of ca. 10% steps). This high reliability of the experiments conducted at the SNS-NSE displays the accuracy of the instrument itself and the latest developments of the evaluation software, which were necessary to interpret such tiny changes of the bending rigidity reliably.

  7. Weak Form Efficiency of the Nigerian Stock Market: An Empirical Analysis (1984 – 2009

    Directory of Open Access Journals (Sweden)

    Pyemo Afego

    2012-01-01

    Full Text Available This paper examines the weak-form of the efficient markets hypothesis for the Nigerian Stock Exchange (NSE by testing for random walks in the monthly index returns over the period 1984-2009. The results of the non-parametric runs test show that index returns on the NSE display a predictable component, thus suggesting that traders can earn superior returns by employing trading rules. Statistically significant deviations from randomness are also suggestive of sub-optimal allocation of investment capital within the economy. The findings, in general, contradict the weak-form of the efficient markets hypothesis, and a range of policy strategies for improving the allocative capacity and quality of the information environment of the NSE are discussed.

  8. S-100b and neuron-specific enolase in patients with fulminant hepatic failure

    DEFF Research Database (Denmark)

    Strauss, Gitte Irene; Christiansen, Michael; Møller, Kirsten

    2001-01-01

    Patients with fulminant hepatic failure (FHF) frequently develop cerebral edema and intracranial hypertension. The aim of this study was to evaluate circulating S-100b and neuron-specific enolase (NSE) levels as markers of neurological outcome in patients with FHF. In a subgroup of patients......, the cerebral flux of S-100b and NSE was measured. We included 35 patients with FHF, 6 patients with acute on chronic liver disease (AOCLD), 13 patients with cirrhosis of the liver without hepatic encephalopathy, and 8 healthy subjects. Blood samples were obtained from catheters placed in the radial artery...... and internal jugular bulb. The net cerebral flux of S-100b and NSE was measured, and the effect of short-term hyperventilation, as well as the effect of high-volume plasmapheresis, on circulating levels of these two biomarkers was determined. Blood levels of S-100b were greater in patients with FHF and AOCLD...

  9. DIAGNOSTIC VALUE OF SERUM PROGRP31-98 IN PATIENTS WITH SMALL-CELL LUNG CANCER

    Institute of Scientific and Technical Information of China (English)

    李昂; 杨谨; 李旭; 李蓉; 王一理; 司履生

    2003-01-01

    Objective To explore the clinical significance of serum level of pro-gastrin-releasing peptide 31-98 (ProGRP31-98) for small-cell lung cancer (SCLC), in comparison with neuron-specific enolase (NSE). Methods Serum level of ProGRP31-98 and NSE was measured by ELISA respectively in 30 patients with SCLC, 30 with non-small cell lung cancer (NSCLC), 15 with benign lung diseases and 15 normal subjects, additionally, 10 SCLC patients after having treatment with chemotherapy were included. The receiver operating characteristic (ROC) curve was used to set the cut-off value and evaluate the diagnostic accuracy. Results The serum level of ProGRP31-98 was higher in patients with SCLC than that in other groups. The SCLC patients with extensive disease had a higher value than the patients with limited disease. In SCLC patients with distant metastases, it was also higher than in those without. Increase in serum ProGRP31-98 and NSE was both seen in SCLC patients, but for the former one, the increase was of much greater compared to the normal controls. Given the cut-off value for ProGRP31-98 was 40ng*L-1 and for NSE 8μg*L-1, their sensitivity of diagnosis in SCLC was 73% and 60%, respectively. The area under ROC curve of ProGRP31-98 was significantly larger than that of NSE. All patients responded to chemotherapy showed marked decrease in ProGRP31-98. Conclusion ProGRP31-98 is a more specific and sensitive marker than NSE in the diagnosis of SCLC.

  10. Freezeouts from neutron-rich nuclear statistical equilibrium

    Science.gov (United States)

    Meyer, B. S.

    1994-07-01

    The correlated isotopic anomalies in Ca-48, Ti-50, and Cr-54 in Ca-Al rich inclusions suggest that these isotopes were coproduced in a neutron-rich Nuclear Statistical Equilibrium (NSE) environment. Nucleosynthesis theory had already reached that conclusion, largely through the NSE calculations. The astrophysical setting for this nucleosynthesis was envisioned to be near the mass cut of a type II (core-collapse) supernova. Work on supernova theory in the past several years has shown that the conditions assumed are not appropriate for the type II mass cut environment. In particular, the densities at given temperatures in the realistic supernova models are about 10x lower than those assumed for the NSE calculations. The lower density in the realistic supernova models means that more free nucleons and alpha particles are present at the time of NSE freezeout, and capture of these light particles on heavier nuclei can substantially alter the NSE abundance pattern. Do such modifications alter the NSE pattern enough to destroy the coproduction of the neutron-rich iron-group nuclei? In order to answer this question, it is necessary to make calculations with a large nucleosynthesis network. I have performed such network calculations for material expanding from an initial density of 3.4 x 107 g/cu cm and temperature 1010 K on an expansion timescale of 0.2 s. These are fairly realistic conditions in light of the supernova models. Freezeout calculations with realistic parameters confirm co-production of the neutron-rich iron-group nuclei. Further calculations are underway to explore the sensitivity of these results to the initial density and the expansion timescale.

  11. Joint Radioisotope Electric Propulsion Studies - Neptune System Explorer

    Science.gov (United States)

    Khan, M. Omair; Amini, Rashied; Ervin, Joan; Lang, Jared; Landau, Damon; Oleson, Steven; Spilker, Thomas; Strange, Nathan

    2011-01-01

    The Neptune System Explorer (NSE) mission concept study assessed opportunities to conduct Cassini-like science at Neptune with a radioisotope electric propulsion (REP) based spacecraft. REP is based on powering an electric propulsion (EP) engine with a radioisotope power source (RPS). The NSE study was commissioned under the Joint Radioisotope Electric Propulsion Studies (JREPS) project, which sought to determine the technical feasibility of flagship class REP applications. Within JREPS, special emphasis was given toward identifying tall technology tent poles, as well as recommending any new RPS technology developments that would be required for complicated REP missions. Based on the goals of JREPS, multiple RPS (e.g. thermoelectric and Stirling based RPS) and EP (e.g. Hall and ion engines) technology combinations were traded during the NSE study to determine the most favorable REP design architecture. Among the findings from the study was the need for >400We RPS systems, which was driven by EP operating powers and the requirement for a long-lived mission in the deep solar system. Additionally multiple development and implementation risks were identified for the NSE concept, as well as REP missions in general. Among the strengths of the NSE mission would be the benefits associated with RPS and EP use, such as long-term power (approx. 2-3kW) at Neptune and flexible trajectory options for achieving orbit or tours of the Neptune system. Although there are still multiple issues to mitigate, the NSE concept demonstrated distinct advantages associated with using REP for deep space flagship-class missions.

  12. Hydrologic Modeling and Parameter Estimation under Data Scarcity for Java Island, Indonesia

    Science.gov (United States)

    Yanto, M.; Livneh, B.; Rajagopalan, B.; Kasprzyk, J. R.

    2015-12-01

    The Indonesian island of Java is routinely subjected to intense flooding, drought and related natural hazards, resulting in severe social and economic impacts. Although an improved understanding of the island's hydrology would help mitigate these risks, data scarcity issues make the modeling challenging. To this end, we developed a hydrological representation of Java using the Variable Infiltration Capacity (VIC) model, to simulate the hydrologic processes of several watersheds across the island. We measured the model performance using Nash-Sutcliffe Efficiency (NSE) at monthly time step. Data scarcity and quality issues for precipitation and streamflow warranted the application of a quality control procedure to data ensure consistency among watersheds resulting in 7 watersheds. To optimize the model performance, the calibration parameters were estimated using Borg Multi Objective Evolutionary Algorithm (Borg MOEA), which offers efficient searching of the parameter space, adaptive population sizing and local optima escape facility. The result shows that calibration performance is best (NSE ~ 0.6 - 0.9) in the eastern part of the domain and moderate (NSE ~ 0.3 - 0.5) in the western part of the island. The validation results are lower (NSE ~ 0.1 - 0.5) and (NSE ~ 0.1 - 0.4) in the east and west, respectively. We surmise that the presence of outliers and stark differences in the climate between calibration and validation periods in the western watersheds are responsible for low NSE in this region. In addition, we found that approximately 70% of total errors were contributed by less than 20% of total data. The spatial variability of model performance suggests the influence of both topographical and hydroclimatic controls on the hydrological processes. Most watersheds in eastern part perform better in wet season and vice versa for the western part. This modeling framework is one of the first attempts at comprehensively simulating the hydrology in this maritime, tropical

  13. The diagnostic efficiency of biomarkers in sporadic Creutzfeldt-Jakob disease compared to Alzheimer's disease

    DEFF Research Database (Denmark)

    Bahl, J.M.; Heegaard, N.H.; Falkenhorst, G.

    2009-01-01

    ) together with the prion protein gene genotype to discriminate patients with sCJD (n=21) from neurological controls (n=164) and Alzheimer's disease (AD) patients (n=49). Low p-tau/t-tau ratio was the best single marker for sCJD with 90% specificity against neurological controls at 86% sensitivity whilst NSE......Laboratory markers have a prominent place among the diagnostic criteria for sporadic Creutzfeldt-Jakob disease (sCJD). Here we investigate the capability of protein 14-3-3, total-tau (t-tau), threonin-181-phosphorylated tau (p-tau), and neuron-specific enolase (NSE) in cerebrospinal fluid (CSF...

  14. Length Scale Dependence of the Dynamic Properties of Hyaluronic Acid Solutions in the Presence of Salt

    Energy Technology Data Exchange (ETDEWEB)

    Horkay, Ferenc; Falus, Peter; Hecht, Anne-Marie; Geissler, Erik (CNRS-UMR); (NIH); (ILL)

    2010-12-07

    In solutions of the charged semirigid biopolymer hyaluronic acid in salt-free conditions, the diffusion coefficient D{sub NSE} measured at high transfer momentum q by neutron spin echo is more than an order of magnitude smaller than that determined by dynamic light scattering, D{sub DLS}. This behavior contrasts with neutral polymer solutions. With increasing salt content, D{sub DLS} approaches D{sub NSE}, which is independent of ionic strength. Contrary to theoretical expectation, the ion-polymer coupling, which dominates the low q dynamics of polyelectrolyte solutions, already breaks down at distance scales greater than the Debye-Hueckel length.

  15. License - SSBD | LSDB Archive [Life Science Database Archive metadata

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    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us SSBD Lice...nse License to Use This Database Last updated : 2016/07/25 You may use this databas...e in compliance with the terms and conditions of the license described below. The license specifies the lice...nse terms regarding the use of this database and the requirements you must follow in using this database. The lice...llows: SSBD c Shuichi Onami (RIKEN Quantitative Biology Center, Laboratory for Developmental Dynamics) licen

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    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us JSNP Lice...nse License to Use This Database Last updated : 2016/02/01 You may use this databas...e in compliance with the terms and conditions of the license described below. The license specifies the lice...nse terms regarding the use of this database and the requirements you must follow in using this database. The lice...llows: JSNP © The University of Tokyo and Japan Science and Technology Agency (JST) licensed under CC Attrib

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    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us SAHG Lice...nse License to Use This Database Last updated : 2016/05/09 You may use this databas...e in compliance with the terms and conditions of the license described below. The license specifies the lice...nse terms regarding the use of this database and the requirements you must follow in using this database. The lice...l Institute of Advanced Industrial Science and Technology (AIST)) licensed under

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    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us eSOL Lice...nse License to Use This Database Last updated : 2012/08/07 You may use this databas...e in compliance with the terms and conditions of the license described below. The license specifies the lice...nse terms regarding the use of this database and the requirements you must follow in using this database. The lice...SOL © Hideki Taguchi (Tokyo Institute of Technology) licensed under CC Attribution-Share Alike 2.1 Japan . T

  19. License - RMOS | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us RMOS Lice...nse License to Use This Database Last updated : 2015/10/27 You may use this databas...e in compliance with the terms and conditions of the license described below. The license specifies the lice...nse terms regarding the use of this database and the requirements you must follow in using this database. The lice...MOS © Shoshi Kikuchi (National Institute of Agrobiological Sciences) licensed under CC Attribution-Share Ali

  20. Neutron spin echo studies of the effects of temperature and pressure in a ternary microemulsion

    CERN Document Server

    Kawabata, Y; Seto, H; Takeda, T; Komura, S; Schwahn, D

    2002-01-01

    In order to clarify the self-assembling mechanisms in complex fluids involving amphiphiles, we have investigated dynamic features of amphiphilic membranes and droplets at high temperature and at high pressure in a ternary microemulsion, consisting of AOT, water, and n-decane. A high-pressure cell for neutron spin echo (NSE) experiments has been improved, and the static and dynamic features of droplets are observed in detail by means of small angle neutron scattering and NSE. It is found that the size fluctuation and the diffusion of droplets are enhanced by increasing temperature, while they are suppressed by increasing pressure. (orig.)

  1. Unravelling the nature of non-specific effects of vaccines-A challenge for innate immunologists

    DEFF Research Database (Denmark)

    Jensen, Kristoffer Jarlov; Benn, Christine Stabell; van Crevel, Reinout

    2016-01-01

    ) of vaccines, including heterologous T-cell reactivity and innate immune memory or 'trained innate immunity', which involves epigenetic reprogramming of innate immune cells. Here, we review the epidemiological evidence for NSE as well as human, animal and in vitro immunological data that could explain......Epidemiological observations have shown that vaccines can influence morbidity and mortality more than can be ascribed to target-disease immunity. A growing number of immunological studies have helped identify possible biological mechanisms to explain these so-called nonspecific effects (NSE...

  2. Derivatives Trading in Emerging Economies with Special Reference to India

    OpenAIRE

    2013-01-01

    In this study The National Stock Exchange of India (NSE) has been taken as the sample exchange since this is the only exchange which provided the derivatives facility throughout the reference period of the study. The reference period of the study has been taken from 2001-02 to 2011-12.MAGR has been employed to determine the growth of derivatives in NSE and this study concludes that the derivatives products such as Stock Futures, Stock Options , Index Options and Index Futures have shown signi...

  3. Effect of Selenium Supplementation on Redox Status of the Aortic Wall in Young Spontaneously Hypertensive Rats

    OpenAIRE

    2015-01-01

    Selenium (Se) is an exogenous antioxidant that performs its function via the expression of selenoproteins. The aim of this study was to explore the effect of varying Se intake on the redox status of the aortic wall in young spontaneously hypertensive rats (SHR). Sixteen male Wistar Kyoto (WKY) rats and nineteen male SHR, 16-week-old, were tested after being given diets with different Se content for eight weeks. They were divided into 4 groups: control groups of WKY NSe and SHR NSe on an adequ...

  4. Modelling time-varying volatility in the Indian stock returns: Some empirical evidence

    Directory of Open Access Journals (Sweden)

    Trilochan Tripathy

    2015-12-01

    Full Text Available This paper models time-varying volatility in one of the Indian main stock markets, namely, the National Stock Exchange (NSE located in Mumbai, investigating whether it has been affected by the recent global financial crisis. A Chow test indicates the presence of a structural break. Both symmetric and asymmetric GARCH models suggest that the volatility of NSE returns is persistent and asymmetric and has increased as a result of the crisis. The model under the Generalized Error Distribution appears to be the most suitable one. However, its out-of-sample forecasting performance is relatively poor.

  5. Fluctuations of serum neuron specific enolase and protein S-100B concentrations in relation to the use of shunt during carotid endarterectomy.

    Directory of Open Access Journals (Sweden)

    Marko Dragas

    Full Text Available To evaluate the changes in serum neuron specific enolase and protein S-100B, after carotid endarterectomy performed using the conventional technique with routine shunting and patch closure, or eversion technique without the use of shunt.Prospective non-randomized study included 43 patients with severe (>80% carotid stenosis undergoing carotid endarterectomy in regional anesthesia. Patients were divided into two groups: conventional endarterectomy with routine use of shunt and Dacron patch (csCEA group and eversion endarterectomy without the use of shunt (eCEA group. Protein S-100B and NSE concentrations were measured from peripheral blood before carotid clamping, after declamping and 24 hours after surgery.Neurologic examination and brain CT findings on the first postoperative day did not differ from preoperative controls in any patients. In csCEA group, NSE concentrations decreased after declamping (P<0.01, and 24 hours after surgery (P<0.01, while in the eCEA group NSE values slightly increased (P=ns, accounting for a significant difference between groups on the first postoperative day (P=0.006. In both groups S-100B concentrations significantly increased after declamping (P<0.05, returning to near pre-clamp values 24 hours after surgery (P=ns. Sub-group analysis revealed significant decline of serum NSE concentrations in asymptomatic patients shunted during surgery after declamping (P<0.05 and 24 hours after surgery (P<0.01, while no significant changes were noted in non-shunted patients (P=ns. Decrease of NSE serum levels was also found in symptomatic patients operated with the use of shunt on the first postoperative day (P<0.05. Significant increase in NSE serum levels was recorded in non-shunted symptomatic patients 24 hours after surgery (P<0.05.Variations of NSE concentrations seemed to be influenced by cerebral perfusion alterations, while protein S-100B values were unaffected by shunting strategy. Routine shunting during surgery for

  6. THE NAVIER-STOKES EQUATIONS IN STREAM LAYER AND ON STREAM SURFACE AND A DIMENSION SPLIT METHODS

    Institute of Scientific and Technical Information of China (English)

    李开泰; 黄艾香

    2002-01-01

    In this paper,we proposal stream surface and stream layer. By using classical tensor calculus, we derive 3-D Navier-Stokes Equations(NSE) in the stream layer under semigeodesic coordinate system, Navier-Stokes equation on the stream surface and 2-D Navier-Stokes equations on a two dimensional manifold. After introducing stream function on the stream surface, a nonlinear initial-boundary value problem satisfies by stream function is obtained, existence and uniqueness of its solution are proven. Based this theory we proposal a new method called "dimension split method" to solve 3D NSE.

  7. Percepciones sobre disponibilidad de alimentos y autorreporte de ingesta alimentaria en mujeres urbanas costarricenses: Un estudio piloto

    OpenAIRE

    Bekelman, Traci A.; Carolina Santamaría-Ulloa; Dufour,Darna L.; Ana Laura Dengo

    2016-01-01

    Antecedentes: Se considera que la disponibilidad y consumo de alimentos han contribuido al aumento de obesidad entre las mujeres de bajo nivel socioeconómico (NSE) en Latinoamérica. En Costa Rica, pocos estudios han investigado cómo la disponibilidad y consumo varían según NSE. Objetivos: Los objetivos de este estudio en San José, Costa Rica, son (1) evaluar percepciones sobre la disponibilidad de alimentos y (2) describir el consumo de alimentos en mujeres que viven en áreas de alto y bajo N...

  8. Transmissibility of H-Type Bovine Spongiform Encephalopathy to Hamster PrP Transgenic Mice.

    Directory of Open Access Journals (Sweden)

    Hiroyuki Okada

    Full Text Available Two distinct forms of atypical bovine spongiform encephalopathies (H-BSE and L-BSE can be distinguished from classical (C- BSE found in cattle based on biochemical signatures of disease-associated prion protein (PrPSc. H-BSE is transmissible to wild-type mice-with infected mice showing a long survival period that is close to their normal lifespan-but not to hamsters. Therefore, rodent-adapted H-BSE with a short survival period would be useful for analyzing H-BSE characteristics. In this study, we investigated the transmissibility of H-BSE to hamster prion protein transgenic (TgHaNSE mice with long survival periods. Although none of the TgHaNSE mice manifested the disease during their lifespan, PrPSc accumulation was observed in some areas of the brain after the first passage. With subsequent passages, TgHaNSE mice developed the disease with a mean survival period of 220 days. The molecular characteristics of proteinase K-resistant PrPSc (PrPres in the brain were identical to those observed in first-passage mice. The distribution of immunolabeled PrPSc in the brains of TgHaNSE mice differed between those infected with H-BSE as compared to C-BSE or L-BSE, and the molecular properties of PrPres in TgHaNSE mice infected with H-BSE differed from those of the original isolate. The strain-specific electromobility, glycoform profiles, and proteolytic cleavage sites of H-BSE in TgHaNSE mice were indistinguishable from those of C-BSE, in which the diglycosylated form was predominant. These findings indicate that strain-specific pathogenic characteristics and molecular features of PrPres in the brain are altered during cross-species transmission. Typical H-BSE features were restored after back passage from TgHaNSE to bovinized transgenic mice, indicating that the H-BSE strain was propagated in TgHaNSE mice. This could result from the overexpression of the hamster prion protein.

  9. Transmissibility of H-Type Bovine Spongiform Encephalopathy to Hamster PrP Transgenic Mice.

    Science.gov (United States)

    Okada, Hiroyuki; Masujin, Kentaro; Miyazawa, Kohtaro; Yokoyama, Takashi

    2015-01-01

    Two distinct forms of atypical bovine spongiform encephalopathies (H-BSE and L-BSE) can be distinguished from classical (C-) BSE found in cattle based on biochemical signatures of disease-associated prion protein (PrPSc). H-BSE is transmissible to wild-type mice-with infected mice showing a long survival period that is close to their normal lifespan-but not to hamsters. Therefore, rodent-adapted H-BSE with a short survival period would be useful for analyzing H-BSE characteristics. In this study, we investigated the transmissibility of H-BSE to hamster prion protein transgenic (TgHaNSE) mice with long survival periods. Although none of the TgHaNSE mice manifested the disease during their lifespan, PrPSc accumulation was observed in some areas of the brain after the first passage. With subsequent passages, TgHaNSE mice developed the disease with a mean survival period of 220 days. The molecular characteristics of proteinase K-resistant PrPSc (PrPres) in the brain were identical to those observed in first-passage mice. The distribution of immunolabeled PrPSc in the brains of TgHaNSE mice differed between those infected with H-BSE as compared to C-BSE or L-BSE, and the molecular properties of PrPres in TgHaNSE mice infected with H-BSE differed from those of the original isolate. The strain-specific electromobility, glycoform profiles, and proteolytic cleavage sites of H-BSE in TgHaNSE mice were indistinguishable from those of C-BSE, in which the diglycosylated form was predominant. These findings indicate that strain-specific pathogenic characteristics and molecular features of PrPres in the brain are altered during cross-species transmission. Typical H-BSE features were restored after back passage from TgHaNSE to bovinized transgenic mice, indicating that the H-BSE strain was propagated in TgHaNSE mice. This could result from the overexpression of the hamster prion protein.

  10. Engang drak de rom

    DEFF Research Database (Denmark)

    Østergaard, Uffe

    2015-01-01

    Med to parallelle erklæringer i 1955 løste Tyskland og Danmark mindretalsspørgsmålet såvel nord som syd for den grænse, der blev trukket i 1920, idet Danmark garanterede rettighederne for det tyske mindretal og Forbundsrepublikken det danske mindretal i Slesvig-Holsten....

  11. AMPA antagonist ZK200775 in patients with acute ischemic stroke - Possible glial cell toxicity detected by monitoring of S-100B serum levels

    NARCIS (Netherlands)

    Elting, JW; Kaste, M; Lees, KR; Diener, HC; Hommel, M; Versavel, M; Teelken, AW; De Keyser, J; Sulter, G.

    2002-01-01

    Background and Purpose-S-100B and neuron-specific enolase (NSE) serum concentrations can be used as peripheral markers of glial cell and neuronal damage, respectively. We investigated these markers in a clinical trial with the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) antagonist Z

  12. Characterization of trehalose aqueous solutions by neutron spin echo

    CERN Document Server

    Branca, C; Magazù, S; Maisano, G; Mangione, A; Pappas, C; Triolo, A

    2002-01-01

    The present work reports neutron spin-echo (NSE) results on aqueous mixtures of trehalose, a naturally occurring disaccharide of glucose, which shows an extraordinary bioprotective effectiveness against dehydration and freezing. The aim of the work is to furnish new results on the dynamics of the trehalose/water system on the nano- and picosecond scales. (orig.)

  13. Predictive value of brain-specific proteins in serum for neurodevelopmental outcome after birth asphyxia.

    Science.gov (United States)

    Nagdyman, Nicole; Grimmer, Ingrid; Scholz, Tristess; Muller, Christian; Obladen, Michael

    2003-08-01

    Brain-specific proteins have been used to detect cerebral injury after birth asphyxia. Previous investigations suggest that serum protein S-100beta, brain-specific creatine kinase (CK-BB), and neuron-specific enolase (NSE) are capable of identifying patients with a risk of developing hypoxic-ischemic encephalopathy. Whether detection of elevated serum concentrations of these proteins reflects long-term neurodevelopmental impairment remains to be investigated. We examined serum protein S-100beta, NSE, and CK-BB at 2, 6, 12, and 24 h after birth in 29 asphyxiated infants and 20 control infants. Neurodevelopmental follow-up examinations were performed at 20 mo of age using the German revision of the Griffiths scales for developmental assessment. Elevated concentrations of serum protein S-100beta, NSE, and CK-BB within 24 h after asphyxia did not correlate with long-term neurodevelopmental delay. We conclude that serum protein S-100beta, NSE, and CK-BB, sampled on the first day of life, is of limited value in predicting severe brain damage after birth asphyxia.

  14. Effect of exercise on Special Aviation Gymnastics Instruments on blood serum levels of selected biochemical indices in cadets.

    Science.gov (United States)

    Wochyński, Zbigniew; Sobiech, Krzysztof Andrzej

    2014-01-01

    Aim of this study was the training effect evaluation on the Special Aviation Gymnastics Instruments (SAGI) on blood metallothionein (MT), zinc (Zn), copper (Cu), protein, neuron-specific enolase (NSE), and physical fitness in the examined cadets. The study comprised 55 cadets, aged 20, divided into two groups: examined group A (N=41) and control group B (N=14). In both groups, blood material was collected twice, i.e. before (baseline) and after training (series I), during (series II), and after completion of training on the SAGI (Series III). Blood serum MT, Zn, Cu, protein, and NSE were assayed with commercially available kits). Physical fitness was assessed with commonly used fitness tests. A significant decrease in serum MT was noted in both groups in all three series of assays after training, except group B in series II. NSE significantly increased in group A in series II after training. NSE activity increased significantly in group B in series I and III. In both groups, a significant decrease in blood serum Zn was noted after training in series I and II. Serum Cu significantly decreased in group A in all three series of assays. Blood serum protein significantly decreased in group A in series III. In series II, blood serum protein increased significantly in both groups. The remaining values were not changed significantly. Training intensity on SAGI lowered serum MT levels after training in comparison with the control group. This might be associated with Zn, Cu, and protein metabolism.

  15. TQM, strategic quality management and quality costs

    DEFF Research Database (Denmark)

    Dahlgaard, Jens Jørn

    1995-01-01

    I artiklen kombineres definitionen af kvalitetsomkostninger med definitionen på TQM og strategisk kvalitetsledelse. Der foreslåes en ny metode til at estimere nedre grænse for kvalitetsomkostningerne, og metodens fordele og svagheder diskuteres. Det viser endvidere, hvordan kvalitetsomkostninger ...

  16. Systemically administered AAV9-sTRAIL combats invasive glioblastoma in a patient-derived orthotopic xenograft model

    Directory of Open Access Journals (Sweden)

    Matheus HW Crommentuijn

    2016-01-01

    Full Text Available Adeno-associated virus (AAV vectors expressing tumoricidal genes injected directly into brain tumors have shown some promise, however, invasive tumor cells are relatively unaffected. Systemic injection of AAV9 vectors provides widespread delivery to the brain and potentially the tumor/microenvironment. Here we assessed AAV9 for potential glioblastoma therapy using two different promoters driving the expression of the secreted anti-cancer agent sTRAIL as a transgene model; the ubiquitously active chicken β-actin (CBA promoter and the neuron-specific enolase (NSE promoter to restrict expression in brain. Intravenous injection of AAV9 vectors encoding a bioluminescent reporter showed similar distribution patterns, although the NSE promoter yielded 100-fold lower expression in the abdomen (liver, with the brain-to-liver expression ratio remaining the same. The main cell types targeted by the CBA promoter were astrocytes, neurons and endothelial cells, while expression by NSE promoter mostly occurred in neurons. Intravenous administration of either AAV9-CBA-sTRAIL or AAV9-NSE-sTRAIL vectors to mice bearing intracranial patient-derived glioblastoma xenografts led to a slower tumor growth and significantly increased survival, with the CBA promoter having higher efficacy. To our knowledge, this is the first report showing the potential of systemic injection of AAV9 vector encoding a therapeutic gene for the treatment of brain tumors.

  17. Citations to Conference Papers Indicate They Are Declining in Importance across All Discipline Areas. A Review of: Lisée, Cynthia, Vincent Larivière and Eric Archambault. ‚Conference Proceedings as a Source of Scientific Information: A Bibliometric Analysis.‛ Journal of the American Society for Information Science and Technology 59.11 (2008: 1776-84.

    Directory of Open Access Journals (Sweden)

    Gaby Haddow

    2009-06-01

    Full Text Available Objective – To compare the impact and ageing of conference proceedings with that of scientific literature in general, as reflected in citation characteristics.Design – Citation analysis.Setting – Thomson’s Science Citation Index, Social Science Citation Index, and Arts and Humanities Citation Index (CD-ROM version.Subjects – Conference proceedings citations.Methods – The Thomson citation indexes were searched to identify all citations to conference proceedings in natural sciences and engineering (NSE and social sciences and humanities (SSH from 1980 to 2005. Keywords in English, Spanish, Italian and German, truncated terms (such as ‘bienn’, single letters (such as P, and numbers were combined to retrieve all possible citations. Additional filters to exclude citations to publications other than proceedings were applied to the P search results, which had accounted for 75% of the total results. The references remaining in the P search set were validated using Google Scholar and WorldCat. Finally, two random samples of 1,000 references were checked manually to determine the extent of false positives and false negatives in the results.Main Results – The study’s findings are presented for NSE and SSH separately, with 1.7% of NSE citations and 2.5% of SSH citations referring to conference proceedings. The total number of citations to proceedings has increased over the period 1980-2005, however, citations to proceedings in NSE and SSH as a proportion of all citations decreased during this time. A small increase in the average number of proceedings citations per paper was found for NSE and SSH. When this increase is compared to the overall increase in references per paper over this period, the share of proceedings citations per paper has decreased. Of all fields in NSE and SSH, only engineering has increased the proportion of proceedings citations, rising from 7% to 10% in the period studied. In 2005, the share of proceedings

  18. Intermediate scattering function for macromolecules in solutions probed by neutron spin echo.

    Science.gov (United States)

    Liu, Yun

    2017-02-01

    The neutron-spin-echo method (NSE) is a powerful technique for studying internal dynamics of macromolecules in solutions because it can simultaneously probe length and time scales comparable to intramolecular density fluctuations of macromolecules. Recently, there has been increased, strong interest in studying protein internal motions using NSE. The coherent intermediate scattering function (ISF) measured by NSE depends on internal, rotational, and translational motions of macromolecules in solutions. It is thus critical, but highly nontrivial, to separate the internal motion from other motions in order to properly understand protein internal dynamics. Even though many experiments are performed at relatively high concentrations, current theories of calculating the ISF of concentrated protein solutions are either inaccurate or flawed by incorrect assumptions for realistic protein systems with anisotropic shapes. Here, a theoretical framework is developed to establish the quantitative relationship of different motions included in the ISF. This theory based on the dynamic decoupling approximation is applicable to a wide range of protein concentrations, including dilute cases. It is also, in general, useful for studying many other types of macromolecule systems studied by NSE.

  19. An Analysis of English Pronunciation of Japanese Learners: From the Viewpoint of EIL

    Science.gov (United States)

    Tominaga, Yuko

    2011-01-01

    The objective of this research is to detect effective factors in English pronunciation teaching from the viewpoint of EIL (English as an International Language). This research is based on the data of the Successful Pronunciation Learners (SPL) selected by the raters, Japanese and native speakers of English (NSE), in Tominaga (2005). On the grounds…

  20. Early detection of response in small cell bronchogenic carcinoma by changes in serum concentrations of creatine kinase, neuron specific enolase, calcitonin, ACTH, serotonin and gastrin releasing peptide

    DEFF Research Database (Denmark)

    Bork, E; Hansen, M; Urdal, P

    1988-01-01

    Creatine kinase (CK-BB), neuron specific enolase (NSE), ACTH, calcitonin, serotonin and gastrin releasing peptide (GRP) were measured in serum or plasma before and immediately after initiation of treatment in patients with small cell lung cancer (SCC). Pretherapeutic elevated concentrations of CK...

  1. Developing a Hypothetical Multi-Dimensional Learning Progression for the Nature of Matter

    Science.gov (United States)

    Stevens, Shawn Y.; Delgado, Cesar; Krajcik, Joseph S.

    2010-01-01

    We describe efforts toward the development of a hypothetical learning progression (HLP) for the growth of grade 7-14 students' models of the structure, behavior and properties of matter, as it relates to nanoscale science and engineering (NSE). This multi-dimensional HLP, based on empirical research and standards documents, describes how students…

  2. NANOLAB at The University of Texas at Austin: A Model for Interdisciplinary Undergraduate Science and Engineering Education

    Science.gov (United States)

    Heitsch, Andrew T.; Ekerdt, John G.; Korgel, Brian A.

    2009-01-01

    The University of Texas at Austin has developed an upper-division undergraduate laboratory called "NANOLAB" to introduce undergraduate science and engineering students to nanoscale science and engineering (NSE) concepts. The NANOLAB is not a stand-alone course offered by a specific department, but rather a laboratory station--or hub--that…

  3. Characterization of trehalose aqueous solutions by neutron spin echo

    Energy Technology Data Exchange (ETDEWEB)

    Branca, C.; Faraone, A.; Magazu' , S.; Maisano, G.; Mangione, A. [Dipartimento di Fisica and INFM, Universita di Messina, PO Box 55, 98166 Messina (Italy); Pappas, C.; Triolo, A. [Hahn-Meitner-Institut, BENSC (NI), Glienicker Strasse, 14109 Berlin (Germany)

    2002-07-01

    The present work reports neutron spin-echo (NSE) results on aqueous mixtures of trehalose, a naturally occurring disaccharide of glucose, which shows an extraordinary bioprotective effectiveness against dehydration and freezing. The aim of the work is to furnish new results on the dynamics of the trehalose/water system on the nano- and picosecond scales. (orig.)

  4. Defining the Nonprofit Sector: Denmark

    DEFF Research Database (Denmark)

    Ibsen, Bjarne; Habermann, Ulla

    Arbejdspapiret, som er en del af det danske bidrag til det internationale forskningsprojekt The Johns Hopkins Comparative Nonprofit Sector Project, redegør for den historiske udvikling af den frivillige sektor i Danmark. Dernæst beskrives forskellige måder af definere og afgrænse den frivillige s...

  5. TQM, strategic quality management and quality costs

    DEFF Research Database (Denmark)

    Dahlgaard, Jens Jørn

    1995-01-01

    I artiklen kombineres definitionen af kvalitetsomkostninger med definitionen på TQM og strategisk kvalitetsledelse. Der foreslåes en ny metode til at estimere nedre grænse for kvalitetsomkostningerne, og metodens fordele og svagheder diskuteres. Det viser endvidere, hvordan kvalitetsomkostninger ...

  6. NCBI nr-aa BLAST: CBRC-TTRU-01-0604 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0604 ref|YP_506424.1| hypothetical protein NSE_0541 [Neorickettsia sen...netsu str. Miyayama] gb|ABD46153.1| putative membrane protein [Neorickettsia sennetsu str. Miyayama] YP_506424.1 0.012 26% ...

  7. NCBI nr-aa BLAST: CBRC-TTRU-01-0739 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0739 ref|YP_506424.1| hypothetical protein NSE_0541 [Neorickettsia sen...netsu str. Miyayama] gb|ABD46153.1| putative membrane protein [Neorickettsia sennetsu str. Miyayama] YP_506424.1 0.086 28% ...

  8. Markers of cerebral damage during delirium in elderly patients with hip fracture

    NARCIS (Netherlands)

    van Munster, B.C.; Korse, C.M.; de Rooij, S.E.; Bonfrer, J.M.; Zwinderman, A.H.; Korevaar, J.C.

    2009-01-01

    ABSTRACT: BACKGROUND: S100B protein and Neuron Specific Enolase (NSE) can increase due to brain cell damage and/or increased permeability of the blood-brain-barrier. Elevation of these proteins has been shown after various neurological diseases with cognitive dysfunction. Delirium is characterized b

  9. Manifestation of the geometric phase in neutron spin-echo experiments

    NARCIS (Netherlands)

    Kraan, W.H.; Grigoriev, S.V.; Rekveldt, M.T.

    2010-01-01

    We show how the geometric (Berry’s) phase becomes manifest on adiabatic rotation of the polarization vector in the magnetic field configuration in the arms in a neutron spin echo (NSE) experiment.When the neutron beam used is monochromatic, a geometric phase collected in one spin-echo arm can be exa

  10. Increased serum neuron specific enolase concentrations in patients with hyperglycemic cortical ischemic stroke

    NARCIS (Netherlands)

    Elting, JW; De Keyser, J; Sulter, G.

    1998-01-01

    A detrimental effect of hyperglycemia in ischemic brain has been demonstrated in laboratory experiments and it has been found that hyperglycemia in ischemic stroke is a predictor of poor outcome. We determined serum neuron specific enolase (NSE) concentrations in 41 consecutive patients with a cereb

  11. Glial and neuronal proteins in serum predict outcome after severe traumatic brain injury.

    NARCIS (Netherlands)

    Vos, P.E.; Lamers, K.J.B.; Hendriks, J.C.M.; Haaren, M. van; Beems, T.; Zimmerman, C.; Geel, W.J.A. van; Reus, H.P.M. de; Biert, J.; Verbeek, M.M.

    2004-01-01

    OBJECTIVE: To study the ability of glial (glial fibrillary acidic protein [GFAP] and S100b) and neuronal (neuron specific enolase [NSE]) protein levels in peripheral blood to predict outcome after severe traumatic brain injury. METHODS: Eighty-five patients with severe traumatic brain injury (admiss

  12. Anomalous chain diffusion in unentangled model polymer nanocomposites

    NARCIS (Netherlands)

    Schneider, G.; Nusser, K.; Neueder, S.; Brodeck, M.; Willner, L.; Farago, B.; Holderer, O.; Briels, W.J.; Richter, D.

    2013-01-01

    We studied unentangled poly(ethylene-alt-propylene) (PEP) in a composite with hydrophobic silica particles as a function of the filler concentration. Our neutron spin echo (NSE) experiments cover both the internal dynamics as well as the center of mass diffusion beyond the Rouse time. The key experi

  13. Structural biomarkers in the cerebrospinal fluid within 24 h after a traumatic spinal cord injury: a descriptive analysis of 16 subjects

    NARCIS (Netherlands)

    Pouw, M.H.; Kwon, B.K.; Verbeek, M.M.; Vos, P.E.; Kampen, A. van; Fisher, C.G.; Street, J.; Paquette, S.J.; Dvorak, M.F.; Boyd, M.C.; Hosman, A.J.F.; Meent, H. van de

    2014-01-01

    Study design:Prospective cohort study.Objectives:To characterize the cerebrospinal fluid (CSF) concentrations of glial fibrillary acidic protein, neuron specific enolase (NSE), S-100beta, tau and neurofilament heavy chain (NFH) within 24 h of an acute traumatic spinal cord injury (SCI), and to corre

  14. Increased lipid peroxidation and neuron specific enolase in treatment refractory schizophrenics.

    Science.gov (United States)

    Medina-Hernández, V; Ramos-Loyo, J; Luquin, S; Sánchez, L F Cerdán; García-Estrada, J; Navarro-Ruiz, A

    2007-10-01

    It is well-known that increased lipid peroxidation and failure of antioxidant mechanisms leads to neuronal damage in schizophrenic patients. However, this neurodegenerative mechanism has not been studied in treatment refractory schizophrenics (TRS). Therefore, the main purpose of this study was to determine neuronal damage in TRS in comparison to non-refractory schizophrenics (NRS) by means of quantitative analysis of lipid peroxidation and neuron specific enolase (NSE) related to the psychopathology severity. Two groups of paranoid schizophrenics, TRS and NRS, and a group of healthy controls (CO) were assembled (n=13). Lipid peroxidation was analyzed through spectrophotometry for quantification of malonaldehyde (MDA) and 4-hydroxynonenal (4-HNE) serum concentrations. As well, serum NSE was quantified by radioimmunoassay (ELSA). Psychopathology was evaluated using the brief psychiatric rating scale (BPRS) and the positive and negative symptoms scale (PANSS). TRS showed significant higher concentrations of lipoperoxides by-products and NSE, than NRS and CO. Clinical scores also revealed a more severe pathology in TRS, than in NRS. Raised lipoperoxidation correlated with higher delusions and emotional withdrawal symptoms, and increased NSE correlated with a lower flow of the conversation and lack of spontaneity. All these results together suggest that TRS patients suffer a greater lipid peroxidation and neuronal damage than NRS, apparently related to worsening of some of the psychiatric symptoms.

  15. Cerebrospinal fluid purine metabolite and neuron-specific enolase concentrations after febrile seizures.

    Science.gov (United States)

    Rodríguez-Núñez, A; Cid, E; Rodríguez-García, J; Camiña, F; Rodríguez-Segade, S; Castro-Gago, M

    2000-10-01

    If febrile seizures cause significant compromise of neuronal metabolism (whether permanent or reversible), this should be reflected in an increase in the cerebrospinal fluid concentrations of neuron-specific enolase (NSE) and/or adenosine triphosphate (ATP) breakdown products. In the present study, AMP, IMP, inosine, adenosine, guanosine, adenine, guanine, hypoxanthine, xanthine, uric acid and NSE concentrations were determined in the cerebrospinal fluid of 90 children 1 h after febrile seizure (73 simple febrile seizures (SFS); 17 complex febrile seizures (CFS)), and in a control group of 160 children. There was no statistically significant difference between the SFS group and the control group for any of the substances determined, suggesting that SFS neither significantly depletes neuronal ATP concentration, nor significantly increases NSE concentration; thus, SFS do not appear to constitute a threat to neuronal integrity. However, patients with CFS showed significantly lower IMP concentrations and significantly higher adenine concentrations than controls, and significantly higher AMP concentrations than SFS patients; these results suggest that CFS may affect energy metabolism in the brain. However, NSE concentrations were normal in the cerebrospinal fluid of both SFS and CFS patients, suggesting that neither type of seizure causes significant neuronal damage, at least early after the seizure.

  16. Silicon Burning: Formation of the Iron Peak Nuclei

    Science.gov (United States)

    Hix, Wm. Raphael; Thielemann, Friederich-Karl

    1993-12-01

    As the most tightly bound nuclei, the 'Iron Peak' nuclei are the culmination of nuclear energy generation in astrophysical environments. Our re-examination of silicon burning, the mechanism by which the nuclei of the iron peak are produced, has revealed a number of potential improvements in the treatment of this ultimate stage of astrophysical nuclear energy generation. Previous work on Nuclear Statistical Equilibrium (NSE), the end state of silicon burning, has neglected the effect that Coulomb screening of capture reactions and their reverse reactions has on the equilibrium distribution, or assumed that these effects cancel, leaving an abundance distribution identical to that predicted in the absence of such screening. We find that the proper treatment of the screening of nuclear reactions in Nuclear Statistical Equilibrium (NSE), can produce significant differences in the relative abundances of the nuclei produced. This is particularly true at high density. Further, results gleaned from simulation work done with a large nuclear network (300 nuclei and 3000 reactions) and from independent calculations of NSE abundance distributions, offer new insights into the quasi-equilibrium mechanism and the approach to NSE. We will discuss methods which use this quasi-equilibrium mechanism to preserve the most important features of the large nuclear network calculations at a significant improvement in computational speed. Such improved methods are ideally suited for hydro- dynamic calculations which involve the production of iron peak nuclei, where the larger network calculation proves unmanageable.

  17. Safety and Efficacy of Erythropoietin in Traumatic Brain Injury Patients: A Pilot Randomized Trial

    Directory of Open Access Journals (Sweden)

    R. Nirula

    2010-01-01

    Conclusions. At the dose used, EPO did not reduce neuronal cell death compared to placebo; however, TBI severity was worse in the EPO group while levels of NSE and S100-B were similar to the less injured placebo group making it difficult to rule out a treatment effect. A larger, balanced study is necessary to confirm a potential treatment effect.

  18. Single versus Serial Measurements of Neuron-Specific Enolase and Prediction of Poor Neurological Outcome in Persistently Unconscious Patients after Out-Of-Hospital Cardiac Arrest - A TTM-Trial Substudy

    DEFF Research Database (Denmark)

    Wiberg, Sebastian; Hassager, Christian; Stammet, Pascal

    2017-01-01

    were included from sites participating in the TTM-trial biobank sub study. NSE was measured at 24, 48 and 72 hours after ROSC and follow-up was concluded after 180 days. The primary end point was poor neurological function or death defined by a cerebral performance category score (CPC-score) of 3 to 5...

  19. Forrest Gump euroliidus / Andres Arrak

    Index Scriptorium Estoniae

    Arrak, Andres, 1958-

    2005-01-01

    Majandusõppejõus tekitab hämmastust, et suveräänse Eesti riigi majandusministeerium kavatseb Euroopa Liidult küsida luba Leedu masuudi sisseostmiseks. Autoris tekitab see küsimuse, kas majandusruum kuhu Eesti kuulub, on ikka konkurentsile üles ehitatud

  20. Dicty_cDB: Contig-U14133-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ta atpB-rbcL intergenic spacer, ... 44 5.4 1 ( DQ209546 ) Trigonidium riopalenque...nse atpB-rbcL intergenic s... 44 5.4 1 ( DQ209517 ) Trigonidium egertonianum atpB-rbcL intergenic spa... 44

  1. Intermediate scattering function for macromolecules in solutions probed by neutron spin echo

    Science.gov (United States)

    Liu, Yun

    2017-02-01

    The neutron-spin-echo method (NSE) is a powerful technique for studying internal dynamics of macromolecules in solutions because it can simultaneously probe length and time scales comparable to intramolecular density fluctuations of macromolecules. Recently, there has been increased, strong interest in studying protein internal motions using NSE. The coherent intermediate scattering function (ISF) measured by NSE depends on internal, rotational, and translational motions of macromolecules in solutions. It is thus critical, but highly nontrivial, to separate the internal motion from other motions in order to properly understand protein internal dynamics. Even though many experiments are performed at relatively high concentrations, current theories of calculating the ISF of concentrated protein solutions are either inaccurate or flawed by incorrect assumptions for realistic protein systems with anisotropic shapes. Here, a theoretical framework is developed to establish the quantitative relationship of different motions included in the ISF. This theory based on the dynamic decoupling approximation is applicable to a wide range of protein concentrations, including dilute cases. It is also, in general, useful for studying many other types of macromolecule systems studied by NSE.

  2. Defining the Nonprofit Sector: Denmark

    DEFF Research Database (Denmark)

    Ibsen, Bjarne; Habermann, Ulla

    Arbejdspapiret, som er en del af det danske bidrag til det internationale forskningsprojekt The Johns Hopkins Comparative Nonprofit Sector Project, redegør for den historiske udvikling af den frivillige sektor i Danmark. Dernæst beskrives forskellige måder af definere og afgrænse den frivillige...

  3. The effect of N-stearoylethanolamine on liver phospholipid composition of rats with insulin resistance caused by alimentary obesity

    Directory of Open Access Journals (Sweden)

    O. V. Onopchenko

    2014-02-01

    Full Text Available We used alimentary obesity-induced insulin resistance (IR model in rats to investigate the influence of N-stearoylethanolamine on the content of phospholipids and their fatty acid composition. Our results show that prolonged high-fat diet triggers considerable aberrations in the composition of main phospholipids in the liver and can be one of the causes of IR in rats. In particular, the increase of phosphatidylcholine, phosphatidylethanolamine and significant decrease of other phospholipids: lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine and diphosphaglicerol were observed. The levels of monounsaturated (erucic, nervonic, oleic and polyunsaturated (eicosatrienoic, docosatrienoic, arachidonic fatty acids were increased; meanwhile the content of diunsaturated acids was decreased. The NSE administration (50 mg/kg of body weight caused restoration of the phospholipids content in the liver of rats with diet-induced IR that highly correlated with the decrease in plasma insulin level and the improvement of insulin sensitivity. Moreover, the effect of NSE was accompanied by the normalization of fatty acids composition of phospholipids that could be related to modulating influen­ce of NSE on the activity of the main fatty acid desaturases. It is known that the imbalance in phospholipid composition of the rat liver causes substantial metabolic alterations that are associated with the development of IR. Accordingly, the compensations of the imbalance by NSE can help to restore insulin sensitivity, inhibit the development of obesity, IR and type 2 diabetes.

  4. NCBI nr-aa BLAST: CBRC-TTRU-01-1228 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1228 ref|ZP_04583985.1| putative membrane protein [Sulfurihydrogenibium yellowstone...nse SS-5] gb|EEP61466.1| putative membrane protein [Sulfurihydrogenibium yellowstonense SS-5] ZP_04583985.1 0.020 24% ...

  5. NCBI nr-aa BLAST: CBRC-TTRU-01-1079 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1079 ref|ZP_04583985.1| putative membrane protein [Sulfurihydrogenibium yellowstone...nse SS-5] gb|EEP61466.1| putative membrane protein [Sulfurihydrogenibium yellowstonense SS-5] ZP_04583985.1 0.022 24% ...

  6. NCBI nr-aa BLAST: CBRC-TTRU-01-1281 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-1281 ref|ZP_04584505.1| putative membrane protein [Sulfurihydrogenibium yellowstone...nse SS-5] gb|EEP60954.1| putative membrane protein [Sulfurihydrogenibium yellowstonense SS-5] ZP_04584505.1 0.022 26% ...

  7. Intraoral myxoid nerve sheath tumour

    NARCIS (Netherlands)

    Schortinghuis, J; Hille, JJ; Singh, S

    2001-01-01

    A case of an intraoral myxoid nerve sheath tumour of the dorsum of the tongue in a 73-year-old Caucasian male is reported. This case describes the oldest patient with this pathology to date. Immunoperoxidase staining for neuronspecific enolase (NSE) and epithelial membrane antigen (EMA) expression d

  8. Hiring Practices Used to Staff the Iraqi Provisional Authorities

    Science.gov (United States)

    2009-01-16

    when the Soectal Assisrant 10 rhe Secretarv o(De&nse (or PublicA @rrs asked hrm to assrst rn tdentifYmg candidates for ~em or adwsor post ttons as...intemational programs and as director of techno logy and business dcvelopmenl Hud a bachdor·s degree in marine eugineering and a master’s in politica l

  9. Biomasse til transportsektoren

    DEFF Research Database (Denmark)

    Mathiesen, Brian Vad; Connolly, David; Lund, Henrik;

    2012-01-01

    Transportsektoren bliver en af de helt store udfordringer i et fremtidigt energisystem baseret på 100 procent vedvarende energi. Selv med de mest optimistiske antagelser om nye teknologier kan behovet næppe dækkes med biobrændstoffer baseret på danske resurser. Løsningen består i at begrænse stig...

  10. Clinical management of small-cell carcinoma of the urinary tract: a 10-year single-center's experience.

    Science.gov (United States)

    Carranza, Omar E; Castañón, Eduardo; Abella, Luis E; Zudaire, María E; Castillo, Ainhoa; Arévalo, Estefanía; Fusco, Juan P; Zudaire, Juan J; Carías, Rafael; Cambeiro, Mauricio; Martínez-Monge, Rafael; Gil-Bazo, Ignacio

    2013-06-01

    Small-cell carcinoma (SCC) comprises 1% of primary bladder tumors and approximately 2% of prostate neoplasms. Metastatic disease at diagnosis is common, and survival outcomes are extremely poor. There is controversy about the ideal clinical management of these patients. The neuron-specific enolase (NSE) serum levels have never been studied in patients with small-cell carcinoma of the urinary tract (SCCUT). We report the clinical outcome of 12 consecutive SCCUT patients treated during the past 10 years. We also study the NSE levels at diagnosis and during treatment. Patients with limited disease (LD) experienced a non-significant longer progression-free survival (PFS) and overall survival (OS) compared with extensive disease (ED) subjects. Patients with bladder SCC showed a significantly higher median PFS compared with prostate SCCUT patients (22 vs. 6 months; P = .034), although that difference did not impact on a significant longer OS. NSE levels decreased during chemotherapy administration in all patients with ED and baseline high levels. Our patients showed a poor prognosis as described in previous studies. A better outcome for patients with bladder SCC compared with prostate SCC could be suggested. Serum NSE levels should be further evaluated to prove its potential use in early diagnosis and treatment monitoring during chemotherapy. Copyright © 2013 Elsevier Inc. All rights reserved.

  11. Midteradskillelse på landevej 447, Vestbjerg-Hjørring

    DEFF Research Database (Denmark)

    Sørensen, Michael; Madsen, Jens Chr. Overgaard; Lahrmann, Harry Spaabæk;

    2005-01-01

    I 2003-2004 blev der på Aalborgvej, landevej 447, mellem Harken og Hjørring foretaget et forsøgsprojekt af Nordjyllands Amt. Projektet omfatter forsøg med alternativ vejafmærkning samt overhalingsforbud, ændring af hastighedsgrænse, ændring af krydsudformning samt etablering bremseheller ved Hark...

  12. Angela Merkel viibis kiirvisiidil Tallinnas

    Index Scriptorium Estoniae

    2008-01-01

    26. augustil 2008 Tallinnas ühepäevasel visiidil viibinud Saksamaa liidukantsler Angela Merkel kohtus president Toomas Hendrik Ilvese ja peaminister Andrus Ansipiga ning pidas loengu Kumu auditooriumis. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu

  13. Angela Merkel pani saksa turistid rõõmust kiljuma ja peatas rongid / Eve Heinla

    Index Scriptorium Estoniae

    Heinla, Eve, 1966-

    2008-01-01

    Saksamaa liidukantsleri Angela Merkeli visiidist Tallinnasse, kohtumisest peaminister Andrus Ansipi ja president Toomas Hendrik Ilvesega. Vt. samas: Ilves: Euroopa seisab otsustamispiiril. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu; Saksa liidukantsler: NATO kaitseb Eestit; Kas turvamisel kasutati ka mõttetut imevitsa?

  14. an intense nigerian stock exchange market prediction using logistic ...

    African Journals Online (AJOL)

    market for a profit making venture into the stock market [2]. ... and Support Vector Machines (SVM); all in an effort to improve the predictions, and thus, make reasonable indicial pronouncements that can guide the economic growth of a country [3]. .... destroyed the correlation relationship existing between the NSE index and.

  15. Zinc or copper deficiency-induced impaired inflammatory response to brain trauma may be caused by the concomitant metallothionein changes

    DEFF Research Database (Denmark)

    Penkowa, Milena; Giralt, M.; Thomsen, Pernille Sjølin;

    2001-01-01

    damage to neurons as indicated by neuron specific enolase (NSE) ir, and the number of cells undergoing apoptosis (measured by TUNEL) was dramatically increased. Zinc deficiency significantly altered brain response to TBI, potentiating the microgliosis and reducing the astrogliosis, while increasing...

  16. Intraoral myxoid nerve sheath tumour

    NARCIS (Netherlands)

    Schortinghuis, J; Hille, JJ; Singh, S

    2001-01-01

    A case of an intraoral myxoid nerve sheath tumour of the dorsum of the tongue in a 73-year-old Caucasian male is reported. This case describes the oldest patient with this pathology to date. Immunoperoxidase staining for neuronspecific enolase (NSE) and epithelial membrane antigen (EMA) expression d

  17. Installation Development Environmental Assessment at Scott Air Force Base, Illinois

    Science.gov (United States)

    2007-05-01

    part of the Proposed Action relative to sustainable building concepts. Where possible, the Air Force will incorporate sustainable building concepts...prOji;!Ct proponents to incorporate sustainable building ~:om:cpts into its construction asld renovation plans. These concepts may include the nse of

  18. Neuron-Specific Enolase as a Predictor of Death or Poor Neurological Outcome After Out-of-Hospital Cardiac Arrest and Targeted Temperature Management at 33°C and 36°C

    DEFF Research Database (Denmark)

    Stammet, Pascal; Collignon, Olivier; Hassager, Christian

    2015-01-01

    BACKGROUND: Neuron-specific enolase (NSE) is a widely-used biomarker for prognostication of neurological outcome after cardiac arrest, but the relevance of recommended cutoff values has been questioned due to the lack of a standardized methodology and uncertainties over the influence of temperatu...

  19. LEAN dig ikke tilbage

    DEFF Research Database (Denmark)

    Weirsøe, Mathilde

    2011-01-01

    Fit, slankere, hurtigst. Lean-konceptet ruller ind over de offentlige institutioner, hvor det skal trimme og effektivisere arbejdsprocesser. Men hvor er den maksimale grænse for effektivitet? Er der et ’tipping point’, hvor Lean ikke længere motiverer, men snarere udmatter...

  20. Neuron-specific enolase, but not S100B or myelin basic protein, increases in peripheral blood corresponding to lesion volume after cortical impact in piglets.

    Science.gov (United States)

    Costine, Beth A; Quebeda-Clerkin, Patricia B; Dodge, Carter P; Harris, Brent T; Hillier, Simon C; Duhaime, Ann-Christine

    2012-11-20

    A peripheral indicator of the presence and magnitude of brain injury has been a sought-after tool by clinicians. We measured neuron-specific enolase (NSE), myelin basic protein (MBP), and S100B, prior to and after scaled cortical impact in immature pigs, to determine if these purported markers increase after injury, correlate with the resulting lesion volume, and if these relationships vary with maturation. Scaled cortical impact resulted in increased lesion volume with increasing age. Concentrations of NSE, but not S100B or MBP, increased after injury in all age groups. The high variability of S100B concentrations prior to injury may have precluded detection of an increase due to injury. Total serum markers were estimated, accounting for the allometric growth of blood volume, and resulted in a positive correlation of both NSE and S100B with lesion volume. Even with allometric scaling of blood volume and a uniform mechanism of injury, NSE had only a fair to poor predictive value. In a clinical setting, where the types of injuries are varied, more investigation is required to yield a panel of serum markers that can reliably predict the extent of injury. Allometric scaling may improve estimation of serum marker release in pediatric populations.

  1. Against Journal Articles for Measuring Value in University Output

    Science.gov (United States)

    Mbali, C.

    2010-01-01

    The following lines of arguments against the metrics of journal articles is developed: (1) Textual output should no longer be main valued output; (2) Digitalization enables other ways of advancing knowledge; (3) Measures by journal article favours the disciplines of Natural Science and Engineering (NSE) and moulds other disciplines of Social…

  2. Increased chromogranin A and neuron-specific enolase in rats with chronic nonbacterial prostatitis induced by 17-beta estradiol combined with castration

    Science.gov (United States)

    Fan, Song; Hao, Zong-Yao; Zhang, Li; Chen, Xian-Guo; Zhou, Jun; Zang, Yi-Fei; Tai, Sheng; Liang, Chao-Zhao

    2014-01-01

    Although chronic nonbacterial prostatitis (CNBP) is a common diagnosis in middle-aged men, the etiology of this disease remains poorly understood. Neuroendocrine cells play an important role in the neuroendocrine regulation of the prostate, and chromogranin A (CgA) and neuron-specific enolase (NSE) are regarded as classic markers of neuroendocrine cells. This study aimed to determine CgA and NSE levels in a CNBP rat model to evaluate the role of neuroendocrine cells in the pathogenesis of CNBP. For developing a CNBP rat model, we examined the ability of 17-beta estradiol and surgical castration alone or in combination to induce CNBP. Histologic inflammation of the prostate was assessed in CNBP-induced rats by hematoxylin-eosin staining, whereas CgA and NSE protein levels were assessed by immunohistochemistry, Western blot analysis, and enzyme-linked immunosorbent assays. Our results showed that 17-beta estradiol combined with castration successfully induced CNBP and that CgA and NSE levels were increased in the prostate of CNBP rats as compared to those without CNBP. These findings indicate that the neuroendocrine regulation mediated by neuroendocrine cells may be involved in the pathogenesis of CNBP. PMID:25120776

  3. Socialpædagogisk kompetence

    DEFF Research Database (Denmark)

    Wahlgren, Bjarne; Mariager-Anderson, Kristina; Hovmand Sørensen, Sia

    2015-01-01

    Behovet for socialpædagogisk kompetence var overskriften på en artikel i Voksenuddannelses jubilæumsnummer i april 2012. I artiklen blev der stillet spørgsmålet: Kan mere fokus på kursisternes trivsel og udbytte begrænse frafald på VUC? I artiklen blev der omtalt et udviklingsarbejde, der omfatte...

  4. High Dynamic Performance Nonlinear Source Emulator

    DEFF Research Database (Denmark)

    Nguyen-Duy, Khiem; Knott, Arnold; Andersen, Michael A. E.

    2016-01-01

    As research and development of renewable and clean energy based systems is advancing rapidly, the nonlinear source emulator (NSE) is becoming very essential for testing of maximum power point trackers or downstream converters. Renewable and clean energy sources play important roles in both terres...

  5. Novel role for gabapentin in neuroprotection of central nervous system in streptozotocine-induced diabetic rats

    Institute of Scientific and Technical Information of China (English)

    Giyasettin BAYDAS; Ertugrul SONKAYA; Mehmet TUZCU; Abdullah YASAR; Emir DONDER

    2005-01-01

    Aim: To investigate the effect of gabapentin on neural [neuron-specific enolase (NSE)] and glial markers [glial fibrillary acidic protein (GFAP) and S100B] in different brain regions of diabetic rats. Methods: Diabetes was induced by a single intraperitoneal injection of streptozotocine (50 mg/kg body weight). Rats in one group received vehicle only for 6 weeks. The levels of GFAP, S 100B, and NSE were determined by immunoblotting in the hippocampus, cortex, and cerebellum. Lipid peroxidation (LPO as malondialdehyde+ 4-hydroxyalkenals) and glutathione (GSH) levels were also determined in the same brain parts. Results: Total and degraded GFAP content and S100B protein expression in different areas of brain tissues significantly increased in diabetic rats compared to control rats. Similarly, NSE levels were also significantly elevated in hyperglycemic rats. In addition, there was a significant increase in LPO levels in the diabetic rat brain compared to control rat brains. Pretreatment with gabapentin prevented the upregulation of GFAP, S 100B, and NSE in all brain regions of diabetic rats. The level of LPO was reduced, but not completely halted, by treatment with gabapentin. Conclusion: These results suggest that diabetes causes glial and neuronal injury, possibly as a result of elevated oxidative stress, and that gabapentin protects neurons and glial cells. Thus, we predict that gabapentin treatment will attenuate the hippocampal and cortical neurodegeneration observed during diabetes mellitus in rats.

  6. Multi-objective Optimization Based Calibration of Hydrologic Model and Ensemble Hydrologic Forecast for Java Island, Indonesia

    Science.gov (United States)

    Yanto, M.; Kasprzyk, J. R.; Rajagopalan, B.; Livneh, B.

    2016-12-01

    This study explores the benefits of multi-objective optimization of Variable Infiltration Capacity (VIC) model for five watersheds in Java, the most populous island in Indonesia. Six objective functions: Nash Sutcliffe Efficiency (NSE), percent bias (PBIAS), logarithmic function of root mean square error (Log-RMSE), predictive efficiency (Pe), percent errors in peak (PEP) and slope of flow duration curve error (SFDCE) were selected as evaluation metrics. These metrics were optimized by tuning four VIC model parameters: infiltration shape parameter (b), fraction of maximum baseflow where nonlinear baseflow begin (Ds), thickness of soil layer 2 (thick2) and thickness of soil layer 3 (thick3). We employed Borg Multiobjective Evolutionary Algorithm (Borg MOEA), an automatic simulation-optimization algorithm, to search for non-dominated solutions. We identified the redundancy between NSE and Log-RMSE, Pe, and PEP through visual inspection of their sensitivity to parameters b and Ds of VIC model and to baseflow index (BFI). Accordingly, we proposed NSE, PBIAS and SFDCE as critical objective functions to represent hydrologic processes in tropical region of Java, Indonesia. Using these three objective functions, we culled the objective functions based on at least - NSE > 0.75, PBIAS resources planning and management.

  7. Prognosis of coma after therapeutic hypothermia: a prospective cohort study.

    NARCIS (Netherlands)

    Bouwes, A.; Binnekade, J.M.; Kuiper, M.A.; Bosch, F.H.; Zandstra, D.F.; Toornvliet, A.C.; Biemond, H.S.; Kors, B.M.; Koelman, J.H.; Verbeek, M.M.; Weinstein, H.C.; Hijdra, A.; Horn, J.

    2012-01-01

    OBJECTIVE: This study was designed to establish the reliability of neurologic examination, neuron-specific enolase (NSE), and median nerve somatosensory-evoked potentials (SEPs) to predict poor outcome in patients treated with mild hypothermia after cardiopulmonary resuscitation (CPR). METHODS: This

  8. Ontogeny of neuro-insular complexes and islets innervation in the human pancreas.

    Directory of Open Access Journals (Sweden)

    Alexandra E. Proshchina

    2014-04-01

    Full Text Available The ontogeny of the neuro-insular complexes (NIC and the islets innervation in human pancreas has not been studied in detail. Our aim was to describe the developmental dynamics and distribution of the nervous system structures in the endocrine part of human pancreas. We used doublestaining with antibodies specific to pan-neural markers (neuron-specific enolase (NSE and S100 protein and to hormones of pancreatic endocrine cells. NSE and S100-positive nerves and ganglia were identified in the human fetal pancreas from gestation week (gw 10 onwards. Later the density of S100 and NSE-positive fibers increased. In adults this network was sparse. The islets innervation started to form from gw 14. NSE-containing endocrine cells were identified from gw 12 onwards. Additionally, S100-positive cells were detected both in the periphery and within some of the islets starting at gw 14. The analysis of islets innervation has shown that the fetal pancreas contained neuro-insular complexes and the number of these complexes was reduced in adults. The highest density of neuro-insular complexes is detected during middle and late fetal periods, when the mosaic islets, typical for adults, form. The close integration between the developing pancreatic islets and the nervous system structures may play an important role not only in the hormone secretion, but also in the islets morphogenesis.

  9. Design of Structural Concrete Members for Shear according to the Theory of Plasticity

    DEFF Research Database (Denmark)

    Jensen, Bjarne Christian; Lapko, Andrzej

    2008-01-01

    Moderne forskydningsberegninger er baseret på plasticitetsteori. Konferencebidraget beskriver diagonaltrykmetoden sådan som den anvendes i Eurocode 2. Behov for beregning af revner fra forskydning forklares sammen med sammenhængen til den øvre grænse for diagonaltrykkets hældning. Behov for dukti...

  10. Deactivation of SCR Catalysts by Additives

    DEFF Research Database (Denmark)

    Castellino, Francesco

    2008-01-01

    Biomasseforbrænding har været anvendt i mere end en dekade i lande som Danmark og Sverige og bliver reguleret af myndighederne med henblik på at begrænse nettoemissionen af CO2. Anvendelsen af biomasse på kraftværkerne skaber dog forskellige problemer, som reducerer effektiviteten af processen og...

  11. Multi-Composite Bioactive Osteogenic Sponges Featuring Mesenchymal Stem Cells, Platelet-Rich Plasma, Nanoporous Silicon Enclosures, and Peptide Amphiphiles for Rapid Bone Regeneration

    Directory of Open Access Journals (Sweden)

    Dongmei Fan

    2011-06-01

    Full Text Available A novel bioactive sponge was created with a composite of type I collagen sponges or porous poly(e-caprolactone (PCL scaffolds, platelet-rich plasma (PRP, BMP2-loaded nanoporous silicon enclosure (NSE microparticles, mineralizing peptide amphiphiles (PA, and mesenchymal stem cells (MSC. Primary MSC from cortical bone (CB  tissue proved to form more and larger colony units, as well as produce more mineral matrix under osteogenic differentiation, than MSC from bone marrow (BM. Coating pre-treatments were optimized for maximum cell adhesion and mineralization, while a PRP-based gel carrier was created to efficiently deliver and retain MSC and  microparticles within a porous scaffold while simultaneously promoting cell recruitment, proliferation, and angiogenesis. Components and composite sponges were evaluated for osteogenic differentiation in vitro. Osteogenic sponges were loaded with MSC, PRP, PA, and NSE and implanted subcutaneously in rats to evaluate the formation of bone tissue and angiogenesis in vivo. It was found that the combination of a collagen sponge with CB MSC, PRP, PA, and the BMP2-releasing NSE formed the most bone and was most vascularized by four weeks compared to analogous composites featuring BM MSC or PCL or lacking PRP, PA, and NSE. This study indicates that CB MSC should be considered as an alternative to marrow as a source of stem cells, while the PRP-PA cell and microparticle delivery system may be utilized for diverse tissue engineering applications.

  12. A STUDY OF NASAL EOSINOPHILIA IN ASTHMA PATIENTS

    Directory of Open Access Journals (Sweden)

    Ramachandra Prabhu

    2014-05-01

    Full Text Available INTRODUCTION: Asthma is an inflammatory, reversible and progressive disease with episodes of exacerbations. Asthma is now considered as single airway disease and hence comprehensive diagnosis, treatment and follow-up of upper and lower respiratory tract is essential. Both the Nasal and Bronchial mucosa are the elements of united airway disease and Eosinophils measured from this mucosa can be an indirect marker of Airway inflammation in asthma. Eosinophil infiltration is hallmark feature of pathogenesis of asthma which is the trigger for the chronic airway inflammation and these are raised in acute exacerbations. Hence assessing Eosinophil is evidence of serological marker for Airway inflammation (AI. Nasal Smear Eosinophils (NSE is used in asthma with Allergic Rhinitis (AR. This study Using Definition of GINA explores the utility of NSE as marker for management of Asthma. METHODS: In this study 100 patients diagnosed with bronchial asthma attending the outpatient department in KIMS were studied. The severity of asthma was assessed as per FEV1 classification- Adapted from 2007 NHLBI Guidelines for the diagnosis and treatment of Asthma Expert panel Report 3. The Statistical analysis was done with SAS 9.2, SPSS 15.0, Stata 10.1, Med Calc 9.0.1, Systat 12.0 and R environment ver.2.11.1.software. RESULTS: Asthma was more common in Females in this study with M: F ratio of 1.04 and Allergic Rhinitis was found in 55% of Asthmatics. Nasal Eosinophilia was seen in 44% in asthmatic group and 49% in Asthma with Allergic Rhinitis. Absolute Eosinophil count (AEC and Differential count (DC for Eosinophils was almost same in both groups. The Sensitivity of NSE with respect to DC is 70% and in AR group with Asthma and in Asthma group with/without AR it was 38%. Similarly the NSE with respect to AEC was 47% in AR group with Asthma whereas in Asthma group with/without AR it was 38%. CONCLUSIONS: The finding confirms that symptoms, Variable airflow Obstruction and

  13. Effects of temporal variability on HBV model calibration

    Directory of Open Access Journals (Sweden)

    Steven Reinaldo Rusli

    2015-10-01

    Full Text Available This study aimed to investigate the effect of temporal variability on the optimization of the Hydrologiska Byråns Vattenbalansavedlning (HBV model, as well as the calibration performance using manual optimization and average parameter values. By applying the HBV model to the Jiangwan Catchment, whose geological features include lots of cracks and gaps, simulations under