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Sample records for nse cyfra21-1 scc-ag

  1. Clinical diagnostic significance of combined detection of serum and pleural effusion levels of CEA, NSE, CYFRA21-1, SCC-Ag in patients with lung cancer

    International Nuclear Information System (INIS)

    Bian Baoxiang; Hu Nan; Wu Fenglei; Yang Chengxi

    2008-01-01

    Objective: To appraise the clinical diagnostic significance of combined detection of serum and chest fluid levels of CEA, NSE, CYFRA21-1 and SCC-Ag in patients with lung cancer. Methods: Serum and pleural effusion contents of CEA, NSE, CYFRA21-1 and SCC-Ag were determined with RIA in 54 patients with lung cancer and 35 patients with benign lung disorders. Results: The serum and pleural effusion contents of CEA, NSE, CYFRA21-1 and SCC-Ag in patients with lung cancer were significantly higher than those in patients with benign lung disorders (P<0.01). The contents of CEA, NSE, CYFRA21-1 and SCC-Ag in patients pleural effusion were significantly higher than those in patients serum (P<0.01). For combined detection of CEA, NSE, CYFRA21-1 and SCC-Ag in serum and pleural effusion, the positive rate was 83.33% and 92.59% respectively. Conclusion: Combined detection of CEA, NSE, CYFRA21-1 and SCC-Ag contents in serum and pleural effusion can increase the positive rate of lung cancer diagnosis. (authors)

  2. Value and significance of tumor markers as CEA, CA125, SCC-Ag, CA199 and CYFRA21-1 in the diagnosis of cervical cancer

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    Xiao-Juan Wang

    2017-09-01

    Full Text Available Objective: To investigate the value and significance of serum CEA, CA125, SCC-Ag, CA199 and CYFRA21-1 in the diagnosis of cervical cancer by comparing the detection of five serum markers. Methods: A total of 108 cases were divided into three groups, including 60 cervical cancerpatients and 20 cervical intraepithelial neoplasiain patients treated in our hospital from September 2015 to September 2016 and 28 healthy women. Radioimmunoassay was used to detect and compare the serum levels of CA125, CA199, CYFRA21-1 and ELISA method was used to detect and compare the serum levels of SCC-Ag, CEA. Results: (1 There was no statistically significant difference in the serum CEA, CA125, SCC-Ag, CA199, CYFRA21-1 levels between CIN group and control group. The serums CEA, CA125, SCC-Ag, CA199, CYFRA21-1 levels of cervical cancer patients were significantly higher than the other two groups. The differences were statistically significant. (2There were statistically significant differences in the serum CEA, CA125, SCC-Ag, CA199, CYFRA21-1 levels between different cervical pathological type groups.The serum CA125, CA199, CEA levels of cervical glandular cancer patients were significantly higher than the other two groups. The differences were statistically significant. The serum SCC-Ag, CYFRA21-1 levels of cervical squamous cancer patients were significantly higher than the other two groups. The differences were statistically significant. Conclusion: The serums CEA, CA125, SCC-Ag, CA199, CYFRA21-1 levels of cervical cancer patients were significantly higher than cervical intraepithelial neoplasiain patients and healthy women. The serum CA125, CA199, CEA levels of cervical glandular cancer patients were significantly higher and the serum SCC-Ag, CYFRA21-1 levels of cervical squamous cancer patients were significantly higher. The five tumor markers can be used in diagnosis of cervical cancer and they are also worthy in distinguishing cervical pathological types.

  3. Diagnostic value of combined determination of serum and pleural effusion CEA, CYFRA21-1 and NSE levels in patients with malignancy

    International Nuclear Information System (INIS)

    Chen Xiaoxiao

    2008-01-01

    Objective: To study the clinical value of combined determination of serum and pleural effusion levels of CEA, CYFRA21-1 and NSE in patients with malignancy. Methods: Serum and pleural effusion CEA, CYFRA21-1 and NSE levels were measured with RIA in 40 patients with malignant and 32 patients with tuberculous pleural effusions. Results: The pleural effusion CEA, CYFRA21-1, NSE levels and pleural effusion serum levels ratio in malignant group were significantly higher than those in tuberculous group (P<0.01). The specificity of CEA (90%) was higher than those in that of CYFRA21-1 and NSE, and the sensitivity of CYFRA21-1 (83%) was higher than that of CEA and NSE. With combined detection of CEA, CYFRA21-1 and NSE, the sensitivity was 90% and the specificity was 89% for diagnosis of malignant pleural effusion. Conclusion: Combined determination of serum and pleural effusion CEA, CYFRA21-1 and NSE levels would be more sensitive for diagnosis of malignant pleural effusion. (authors)

  4. The concentration of CYFRA 21-1, NSE and CEA in cerebro-spinal fluid can be useful indicators for diagnosis of meningeal carcinomatosis of lung cancer.

    Science.gov (United States)

    Wang, Peng; Piao, Yingzhe; Zhang, Xiaohui; Li, Wenliang; Hao, Xishan

    2013-01-01

    We aimed to investigate the concentration of CYFRA 21-1, NSE and CEA in cerebro-spinal fluid (CSF) and to explore their clinical value in the meningeal carcinomatosis (MC) of lung cancer. So that, sensitive and specificity of CSF examination can be improved in the initial diagnosis of MC. A total of 35 lung cancer patients and 35 patients with benign brain tumor in the same period enrolled in this study. The concentrations of tumor markers CEA, CYFRA 21-1 and NSE in CSF and peripheral blood were examined. The concentrations of three tumor markers of CYFRA 21-1, NSE and CEA in blood serum and CSF were obviously higher than that of benign disease group. In MC patients, the concentrations of three tumor markers of CYFRA 21-1, NSE and CEA in blood serum were significant lower than that in CSF. The maximum of Youden's index was identified as the cutoff value of indicator of MC in three tumor markers in CSF which were CEA > 4.7 μg/L, NSE > 14.6 μg/L and CYFRA21-1 > 5.5 μg/L respectively. Based on the cutoff values, the CEA had the highest sensitivity while the CYFRA21-1 had the highest specificitiy. Three tumor markers in the CSF had higher positive rate than those in blood serum. We combined the levels of CEA, NSE and CYFRA21-1 in CSF to diagnosis of MC. Positive of CEA or CYFRA21-1 had the greatest sensitivity of 100% while the specificity of 91.4%; the positive of both CEA and CYFRA21-1 had the highest specificity of 100% while the sensitivity of 74.3%. Both positive predictive value and negative predictive value were 100% when combination positive were confirmed when the all three markers were positive. The combination of CEA and CYFRA21-1 can be recommended in early screening of meningeal carcinoma. Especially, for the patient who was difficult to be diagnosed by CSF histology and MRI, it will be a useful auxiliary marker in diagnosis of MC. The combination of CEA, NSE and CYFRA21-1 can be an effective clinically confirmation and exclusively diagnose indictor of

  5. Clinical significance of combined determination of several tumor markers (including CYFRA21-1, NSE, CA-50 and CEA) in patients with pulmonary cancer

    International Nuclear Information System (INIS)

    Zhu Mingfeng; Wen Chijun; Zhu Cuiying

    2005-01-01

    Objective: To enhance the diagnosis of pulmonary cancer by determination of optimal combinations of various tumor markers. Methods: Serum CYFRA 21-1 , NSE, CA-50 (with RIA) and CEA (with CLIA) contents were determined in 107 patients with various types of pulmonary cancers, 66 patients with various benign pulmonary diseases and 59 controls. Results: It was revealed that CYFRA 21-1 determination was most sensitive for detection of squamous cell carcinoma. The same was true for CEA in the detection of adenocarcinoma. NSE determination was very specific for small cell carcinoma. Combined determinations of either CYFRA 21- l + NSE or CYFRA 21-1 + NSE + CEA were excellent for general screening. Conclusion: Combined determination of these tumor markers could be applied expediently as supplementary diagnostic measure for pulmonary malignancies. (authors)

  6. Clinical significance of combined detection of CYFRA21-1, NSE and CEA in classification and staging of patients with lung cancer

    International Nuclear Information System (INIS)

    Hu He; Li Yanhua; Liang Weida; Zhang Qin

    2011-01-01

    To explore clinical value of combined detection of CYFRA21-1, NSE and CEA in classification and staging of patients with lung cancer, the CYFRA21-1, NSE and CEA levels in pleural effusion in 330 patients with lung cancer and in 43 patients with benign were detected by the electrochemiluminescence. The results showed that CYFRA21-1, NSE and CEA levels in pleural effusion in patients with lung cancer group were significantly higher than that of in benign group (P<0.01). The positive rate of tumor markers in different pathological type lung cancer were different,which CYFRA21-1 positive rate in squamous cell cancer group was highest with 65.5%; CEA positive rate in glands cancer group was supreme with 65.0%; the NSE positive rate in differentiation cancer group was highest with 79.5%. The positive rate in three markers combined detection was higher than that in one item detection. The tumor marker levels in lung cancer were positively related with clinical staging. The higher of tumor marker levels and the more late of clinical staging, and the clinical III∼IV period was obviously higher than that I∼II period (P<0.05). The combined detection of CYFRA21-1, NSE and CEA may enhance the positive rate in lung cancer detection, and may have significant clinical value in the classification and staging of patients with lung cancer. (authors)

  7. Clinical study of combined determination of CYFRA21-1, CEA, NSE in the patients with lung cancer. A retrospective analyses in 239 cases

    International Nuclear Information System (INIS)

    Jin Xiumu; Xie Wenhui; Yu Zhichang; Zhang Peiling

    2000-01-01

    Three tumor markers or CEA, CYFRA 21-1 and NSE were assayed in 239 cases with lung cancer (adenocarcinoma 129, squamous-cell carcinoma 59, small cell lung cancer 35, mixed type of adenocarcinoma and squamous-cell carcinoma 16) and 66 cases with benign lung disease. The positive rate of CEA, CYFRA 21-1 and NSE for detecting lung cancer were 51.4%, 43.5% and 48.1% respectively. It seemed there was a relationship between the sensitivity and the pathologic patterns of lung cancer. The highest diagnostic sensitivities were 76.3% for CYFRA 21-1 in the detection of squamous-cell carcinoma, 57.4% for CEA in adenocarcinoma and 94.3% for NSE in the small cell lung cancer respectively. In 49 cases with pleural effusion (adenocarcinoma 27, small cell lung cancer 7, benign disease 15), three tumor markers in serum and pleural fluid were both measured. The results indicated that the sensitivity of the CEA and CYFRA 21-1 in pleural fluid in patients with adenocarcinoma was superior to serum. The detection of the NSE in pleural fluid was a very reliable method in diagnosing the small cell lung cancer. The sensitivity and specificity of CEA, CYFRA 21-1 and NSE in different pathologic patterns of lung cancer was compared and also the false positive and false negative in benign lung disease. Moreover, the clinical role and necessity of combined determination were also discussed

  8. Clinical application of combined determination of serum/chest fluid CEA, CYFRA21-1 and NSE levels for diagnosis of lung cancer

    International Nuclear Information System (INIS)

    Zhu Yalin; Zhu Xiangping

    2004-01-01

    Objective: To explore the clinical value of combined determination of serum/chest fluid CEA,CYFRA21-1 and NSE levels in the diagnosis of lung cancer. Methods: Combined determination of serum levels of CEA,CYFRA21-1 and NSE were done in 53 patients with lung cancer , 26 patients with benign lung diseases and 37 controls. Levels of these three tumor markers were also determined in the pleural fluid present in 33 of the 53 lung cancer patients. Results: In the controls, the serum levels of CEA, CYFRA21-1 and NSE were 2.68 ± 1.75, 1.52 ± 0.86 and 8.77 ± 4.13 ng/ml respectively. In patients with benign lung diseases, the values were 5.48 ± 3.26, 5.32 ± 2.27 and 15.21 ± 11.36 ng/ml respectively. In patients with lung cancer, they were 24.95 ± 18.36, 17.81 ± 11.35 and 19.85 ± 14.22 ng/ml respectively. Serum levels of all these three markers were significantly higher in patients with lung cancer than those in the controls (P 0.05). Levels of all these markers were significantly higher in patients with benign lung diseases than those in the controls (P 0.05); only levels of CYFRA21-1 were significantly higher (P<0.01). Sensitivity of the respective marker in pleural fluid was higher than that in serum. Conclusion: For diagnosis of lung cancer, determination of serum CYFRA21-1 levels or combined determination of the three tumor markers would be most valuable to test levels in pleural fluid, if available, would be more sensitive. (authors)

  9. Combined detection of CEA, CYFRA21-1, NSE and SF levels in chest effusion fluid for differentiation of malignant hydrothorax from tuberculous hydrothorax

    International Nuclear Information System (INIS)

    Wang Jianguo; Zhai Shijun; Liu Ruihua; Quan Min

    2003-01-01

    Objective: To improve the diagnostic accuracy in the differentiation of malignant hydrothorax from tuberculous hydrothorax by combined detection of the levels of the four tumor markers in chest effusion fluid. Methods: The chest fluid levels of the four tumor markers were determined with RIA (for CYFRA21-1 and NSE) and chemiluminescence method (for CEA and SF) in 69 patients with tuberculous hydrothorax and 107 patients with malignant hydrothorax. Results: The positive rate and mean levels of the four tumor markers in malignant chest fluid were significantly higher than those in tuberculous chest fluid (p<0.01). Positive rate of combined detection in malignant chest fluid was 95.33%. Conclusion: Detection of chest fluid CEA, CYFRA21-1, NSE and SF levels is very useful for the differentiation of malignant hydrothorax from tuberculous hydrothorax. Combined detection of the four markers will greatly improve the diagnostic accuracy

  10. Combined detection of AM, CYFRA21-1, NSE and CEA levels in pleural effusion for differentiation of malignant from tuberculous pleural effusion

    International Nuclear Information System (INIS)

    Yu Hua; Zhu Wenru; Sun Shuhong; Xu Shuhua; Yu Hui

    2005-01-01

    The level s of four tumor markers (AM, CYFRA21-1, NSE and CEA) pleural effusion in plearal effusion were determined by RIA in 52 patients with tuberculous pleural effusion and 74 patients with malignant pleural effusion. The results showed that the levels of the four tumor markers in malignant pleural effusion were significantly higher than those in tuberculous pleural effusion. Combined detection of the four tumor markers could improve the diagnostic sensitivity and the accuracy to 90.5% and 92.9%, respectively (P<0.01). Detection of AM, CYFRA21-1, NSE and CEA levels in pleural effusion is very useful for the differentiation of malignant from tuberculous pleural effusion. Combined detection of the four markers may greatly improve the diagnostic accuracy. (authors)

  11. Clinical diagnostic value of combined determination of serum TSGF, CEA, CYFRA21-1 and NSE levels in patients with lung cancer

    International Nuclear Information System (INIS)

    Sun Qihe; Sun Bin

    2008-01-01

    Objective: To explore the clinical diagnostic value of combined determination of serum TSGF, CEA, CYFRA21-1 and NSE levels in patients with lung cancer. Methods: The four serum tumor markers were determined with RIA or other methods in 179 patients with lung cancer, 48 patients with benign lung diseases and 51 controls. Results: The serum levels of all these four markers in the cancer patients were significantly higher (P<0.05-P<0.01) than those in patients with benign pulmonary disorders with the exception of: (1) Serum TSGF, CEA and NSE levels in patients with stage I and II squamous cell carcinoma (n=37) and (2) serum NSE levels in patients with stage I and II adenocarcinoma (n=32). As a whole, the levels of the markers increased along with the increase of the severity of the disease. Conclusion: For the early diagnosis of lung cancer, serum CYFRA21-1 levels determination is the most specific and serum NSE levels determination for diagnosis in patients with NSCLC is the least sensitive. The combined determination of tumor markers is the best choice. (authors)

  12. The clinical value of 99Tcm-HL91 hypoxia imaging combined with the detection of serum NSE, CEA and CYFRA21-1 in the diagnosis of lung cancer

    International Nuclear Information System (INIS)

    Li Xiaohong; Wang Yingqiu; Sun Gaofeng; Zhang Anyu; Zuo Changjing

    2012-01-01

    Objective: To investigate the value of 99 Tc m -HL91 hypoxia imaging combined the detection of serum neuron-specific enolase (NSE), carcinoembryonic antigen(CEA) and cytokeratin 19 fragment (CYFRA21-1) in the diagnosis of lung cancer. Methods: 63 patients with lung neoplasm were carried out 99 Tc m -Hail hypoxia imaging, and the serum tumor markers levels of Nose, Cea and CYFRA21-1 were detected. The sensitivity, specificity and accuracy of each and combined method were calculated respectively and compared with each other. Results: The sensitivity,specificity and accuracy in diagnosis of lung cancer with 99 Tc m -HL91 hypoxia imaging were 85.4%, 100% and 88.9% respectively; and with serum tumor markers NSE, CEA and CYFRA21-1 detection were 72.9%, 86.7% and 76.1% respectively. Compared with the detection of serum tumor marker levels of NSE, CEA and CYFRA21-1, 99 Tc m -HL91 hypoxia imaging had the higher sensitivity and specificity,and similar accuracy. The sensitivity, specificity and accuracy of combined two methods in diagnosis of lung cancer were 95.8%, 86.7% and 93.6% respectively. The sensitivity and accuracy of the combined method were significantly increased than single method (P 0.05). Conclusion: 99 Tc m -HL91 hypoxia imaging and the detection of serum level of NSE, CEA and CYFRA21-1 have an important diagnostic value, and their combination could improve the sensitivity and accuracy in the diagnosis of lung cancer. (authors)

  13. Mucins CA 125, CA 19.9, CA 15.3 and TAG-72.3 as tumor markers in patients with lung cancer: comparison with CYFRA 21-1, CEA, SCC and NSE.

    Science.gov (United States)

    Molina, Rafael; Auge, Jose Maria; Escudero, Jose Miguel; Marrades, Ramon; Viñolas, Nuria; Carcereny, Emilio; Ramirez, Jose; Filella, Xavier

    2008-01-01

    Tumor marker serum levels were prospectively studied in 289 patients with suspected, but unconfirmed, lung cancer and in 513 patients with lung cancer [417 non-small cell lung cancer (NSCLC) patients and 96 small cell lung cancer (SCLC) patients]. In patients with benign disease, abnormal serum levels were found for the following tumor markers: CEA (in 6.6% of patients); CA 19.9 (6.2%); CA 125 (28.7%); NSE (0.7%); CYFRA (8.7%); TAG-72.3 (4.2%); SCC (3.5%), and CA 15.3 (3.5%). Excluding patients with renal failure or liver diseases, tumor marker specificity improved with abnormal levels in 0.5% for NSE, 0.9% for SCC, 2.8% for CEA, CA 15.3 and TAG-72.3, 3.8% for CA 19.9, 4.2% for CYFRA and 21.4% for CA 125. Excluding CA 125, one of the markers was abnormal in 15% of patients without malignancy. Tumor marker sensitivity was related to cancer histology and tumor extension. NSE had the highest sensitivity in SCLC and CYFRA and CEA in NSCLC. Significantly higher concentrations of CEA, SCC, CA 125, CA 15.3 and TAG-72.3 were found in NSCLC than in SCLC. Likewise, significantly higher CEA (p tumors. Using a combination of 3 tumor markers (CEA, CYFRA 21-1 in all histologies, SCC in squamous tumors and CA 15.3 in adenocarcinomas), a high sensitivity may be achieved in all histological types. Tumor markers may be useful in the histological differentiation of NSCLC and SCLC. Using specific criteria for the differentiation of SCLC and NSCLC, the sensitivity was 84.2 and 68.8%, the specificity was 93.8 and 99.7%, the positive predictive value was 98.3 and 98.5% and the negative predictive value was 57.7 and 93.3%, respectively. Copyright 2008 S. Karger AG, Basel.

  14. CYFRA 21.1 in bronchoalveolar lavage of idiopathic pulmonary fibrosis patients.

    Science.gov (United States)

    Vercauteren, Inge M; Verleden, Stijn E; McDonough, John E; Vandermeulen, Elly; Ruttens, David; Lammertyn, Elise J; Bellon, Hannelore; De Dycker, Els; Dooms, Christophe; Yserbyt, Jonas; Verleden, Geert M; Vanaudenaerde, Bart M; Wuyts, Wim A

    2015-01-01

    Idiopathic pulmonary fibrosis (IPF) is one of the most aggressive forms of interstitial lung diseases, however, clinically relevant biomarkers of diagnosis or prognosis are lacking. In this study, we investigated the levels of a fragment of Cytokeratin 19 (CYFRA 21.1) in bronchoalveolar lavage (BAL) of IPF patients at time of diagnosis. We further evaluated associations between CYFRA 21.1, pulmonary function evolution, mortality, and BAL cell count. Using the Lumipulse® G1200, CYFRA 21.1 was measured in BAL samples of 81 IPF patients and 9 controls. Based upon the median detected level (1.2 ng/mL) of CYFRA 21.1 in IPF patients, they were subdivided into an IPF CYFRA 21.1 low group (≤ 1.2 ng/mL) and IPF CYFRA 21.1 high group (> 1.2 ng/mL). The CYFRA 21.1 levels were significantly higher in BAL of IPF patients compared to controls (P = .0015).Worse survival was observed, but no changes in pulmonary function, for IPF patients with high CYFRA 21.1 levels versus patients with low CYFRA 21.1 levels [P = .030, HR: 0.41, (0.18-0.92)[. The CYFRA 21.1 level correlated with both neutrophils (%: R = 0.60, P < .0001; #: R = 0.47, P < .0001) and eosinophils (%: R = 0.38, P = .0005; #: R = 0.30, P < .0072). CYFRA 21.1 is increased in BAL of IPF patients. IPF patients with a high CYFRA 21.1 concentration have a worse survival. CYFRA 21.1 levels correlate with eosinophils and neutrophils. Further studies are warranted in using CYFRA 21.1 as a biomarker for IPF prognosis.

  15. Analysis in patients of the Hospital A.C. Camargo of two tumors markers: CA-72.4 and CYFRA-21.1

    International Nuclear Information System (INIS)

    Barbuto, Jose Alexandre Marzagao; Oda, Emilia Emiko; Oliveira, Ricardo Manoel de

    1995-01-01

    The behavior of two tumor markers, CA-72.4 and CYFRA-19.9 was analysed in patients of the Hospital A.C. Camargo. The measurement of CA-272.4 serum levels was performed by an automated chemo luminescence assay in 49 patients and proved useful in the two groups analyzed, patients with esophageal tumors and with gastric tumors. In both cases, there was an increase in sensitivity when measurements of this marker was added to that of carcinoembryonic antigen (CEA) and of CA-19.9. The most sensitive associations were of CA-72.4 and CA-19.9 for esophageal tumors and of CA-72.4 and CEA for the gastric tumors. The maker CYFRA-21.1 was analyzed by a radioimmunoassay in 102 normal blood donors and in 20 patients with lung cancer. Among these, CYFRA-21.1 was altered in 55% of the cases. Therefore, CYFRA-21.1 could be considered, as the neuron-specific enolase (NSE), very useful tool for the follow-up lung cancer patients. (author)

  16. Analysis in patients of the Hospital A.C. Camargo of two tumors markers: CA-72.4 and CYFRA-21.1; Analise em pacientes do Hospital A.C. Camargo de dois marcadores tumorais: CA-72.4 e CYFRA-21.1

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    Barbuto, Jose Alexandre Marzagao; Oda, Emilia Emiko; Oliveira, Ricardo Manoel de [Fundacao Antonio Prudente, Sao Paulo, SP (Brazil). Hospital A.C. Camargo

    1995-03-01

    The behavior of two tumor markers, CA-72.4 and CYFRA-19.9 was analysed in patients of the Hospital A.C. Camargo. The measurement of CA-272.4 serum levels was performed by an automated chemo luminescence assay in 49 patients and proved useful in the two groups analyzed, patients with esophageal tumors and with gastric tumors. In both cases, there was an increase in sensitivity when measurements of this marker was added to that of carcinoembryonic antigen (CEA) and of CA-19.9. The most sensitive associations were of CA-72.4 and CA-19.9 for esophageal tumors and of CA-72.4 and CEA for the gastric tumors. The maker CYFRA-21.1 was analyzed by a radioimmunoassay in 102 normal blood donors and in 20 patients with lung cancer. Among these, CYFRA-21.1 was altered in 55% of the cases. Therefore, CYFRA-21.1 could be considered, as the neuron-specific enolase (NSE), very useful tool for the follow-up lung cancer patients. (author) 6 refs., 3 figs., 3 tabs.

  17. Diagnostic value of CEA and CYFRA 21-1 tumor markers in primary lung cancer.

    Science.gov (United States)

    Okamura, Kyoko; Takayama, Koichi; Izumi, Miiru; Harada, Taishi; Furuyama, Kazuto; Nakanishi, Yoichi

    2013-04-01

    Lung cancer is sometimes difficult to differentiate from benign lung diseases expressing nodular shadow in imaging study. We assessed the diagnostic value of two commonly used tumor markers in distinguishing primary lung cancer from benign lung disease. The serum levels of carcinoembryonic antigen (CEA) and cytokeratin 19 fragments (CYFRA 21-1) were retrospectively analyzed in 655 lung cancer patients and 237 patients with benign lung disease. The standard cut-off levels of 3.2 ng/mL CEA and 3.5 ng/mL CYFRA 21-1 and twice these respective levels (6.4 ng/mL and 7.0 ng/mL) were used. CEA and CYFRA 21-1 levels were elevated in 32% and 11% of benign lung disease patients, respectively. CEA sensitivity and specificity for lung cancer diagnosis was 69% and 68% respectively, while that for CYFRA 21-1 was 43% and 89%, respectively. Thus, the combined value for the specificity of the two tumor markers was greater than either alone. Patients were grouped depending on their hospital status, and prevalence rates were determined. The prevalence rate of lung cancer in admitted patients was 51%, the prevalence rate of lung cancer in outpatients was 12%, and the prevalence rate of lung cancer identified during health check-ups was 0.1%. Positive predictive values (PPVs) were calculated using Bayes' theorem, and varied with the serum tumor marker and prevalence rate: PPVs of CEA [prevalence rate] were 69.2% [51%], 22.7% [12%], and 0.22% [0.1%], while PPVs of CYFRA 21-1 were 80.3% [51%], 34.8% [12%], and 0.39% [0.1%]. However, PPVs for lung cancer diagnosis at a prevalence rate of 51% were 87.3% or higher when the patient exhibited positive CEA and CYFRA 21-1, or CEA or CYFRA 21-1 levels twice the standard cut-off. Our results indicate that CEA and CYFRA 21-1 are reliable serum tumor markers for the diagnosis of lung cancer in addition to CT scans when combined or used individually at twice the standard cut-off level in high prevalence rate groups. The prevalence rate should

  18. Serum CYFRA 21-1 in Egyptian women with breast cancer | Ebied ...

    African Journals Online (AJOL)

    Introduction: Cytokeratin fragment 21.1 (CYFRA 21.1) assay detects a serum fragment of cytokeratin 19 (CK19) and is employed in the diagnosis and management of lung cancer, particularly of squamous cell histotype. Breast carcinoma has been demonstrated to express CK19 fragments in the primary and metastatic ...

  19. Clinical Usefulness of Serum CYFRA 21-1 in Patients with Colorectal Cancer

    International Nuclear Information System (INIS)

    Lee, Jai Hyuen

    2013-01-01

    Among diverse tumor markers, pretreatment evaluation and follow-up detection of recurrence in colorectal cancer are generally evaluated by serum carcinoembryonic antigen (CEA) levels. However, there have been some reports about the low accuracy and high false-positive results of CEA in colorectal cancer. We investigated the clinical utilities of CYFRA 21-1 by comparing CEA and cancer antigen 19-9 (CA 19-9) in pretreatment and recurrent colorectal cancer. Using a solid-phase immunoradiometric assay, serum levels of CYFRA 21-1, CEA and CA 19-9 were analyzed in 132 patients with primary colorectal cancer, 124 healthy controls, 104 patients with benign colorectal disease and 19 patients with recurrent colorectal cancer. We determined three different cutoff values to evaluate the sensitivity of diagnostic performance in pretreatment and recurrent colorectal cancer. CYFRA 21-1 (≥ 1.13 ng/ml) had a sensitivity of 47 %, compared with 37 % for CEA (≥ 3.05 ng/ml) and 32.6 % for CA 19-9 (≥ 23.1 ng/ml) in the initial staging of primary colorectal cancer. Using different cutoff values, CYFRA 21-1 showed higher sensitivity for pretreatment colorectal cancer than CEA and CA 19-9 in adenocarcinoma and adenosquamous carcinoma of this study. A mildly significant correlative relationship was noted between Dukes' stages and three tumor markers (p<0.01). The areas under the receiver operating characteristic curves of CYFRA 21-1, CEA and CA 19-9 were 0.81±0.03, 0.74±0.03 and 0.62±0.04, respectively, for discriminating colorectal cancer patients from patients with benign colorectal disease. In addition, CYFRA 21-1 was determined as the most sensitive tumor marker for evaluating recurrent colorectal cancer for all cutoff values. This study showed that CYFRA 21-1 could be a useful and dependable tumor marker for pretreatment and recurrent colorectal cancer. Further prospective studies on its usefulness with respect to the prognosis and utility of combined tumor markers are needed

  20. Clinical Usefulness of Serum CYFRA 21-1 in Patients with Colorectal Cancer

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    Lee, Jai Hyuen [Dankook Univ. Medical College, Yongin (Korea, Republic of)

    2013-09-15

    Among diverse tumor markers, pretreatment evaluation and follow-up detection of recurrence in colorectal cancer are generally evaluated by serum carcinoembryonic antigen (CEA) levels. However, there have been some reports about the low accuracy and high false-positive results of CEA in colorectal cancer. We investigated the clinical utilities of CYFRA 21-1 by comparing CEA and cancer antigen 19-9 (CA 19-9) in pretreatment and recurrent colorectal cancer. Using a solid-phase immunoradiometric assay, serum levels of CYFRA 21-1, CEA and CA 19-9 were analyzed in 132 patients with primary colorectal cancer, 124 healthy controls, 104 patients with benign colorectal disease and 19 patients with recurrent colorectal cancer. We determined three different cutoff values to evaluate the sensitivity of diagnostic performance in pretreatment and recurrent colorectal cancer. CYFRA 21-1 (≥ 1.13 ng/ml) had a sensitivity of 47 %, compared with 37 % for CEA (≥ 3.05 ng/ml) and 32.6 % for CA 19-9 (≥ 23.1 ng/ml) in the initial staging of primary colorectal cancer. Using different cutoff values, CYFRA 21-1 showed higher sensitivity for pretreatment colorectal cancer than CEA and CA 19-9 in adenocarcinoma and adenosquamous carcinoma of this study. A mildly significant correlative relationship was noted between Dukes' stages and three tumor markers (p<0.01). The areas under the receiver operating characteristic curves of CYFRA 21-1, CEA and CA 19-9 were 0.81±0.03, 0.74±0.03 and 0.62±0.04, respectively, for discriminating colorectal cancer patients from patients with benign colorectal disease. In addition, CYFRA 21-1 was determined as the most sensitive tumor marker for evaluating recurrent colorectal cancer for all cutoff values. This study showed that CYFRA 21-1 could be a useful and dependable tumor marker for pretreatment and recurrent colorectal cancer. Further prospective studies on its usefulness with respect to the prognosis and utility of combined tumor markers are

  1. Correlation between Changes in Serum Level of CEA and CYFRA 21-1 and Objective Response of Chemotherapy

    Directory of Open Access Journals (Sweden)

    Xinlin MU

    2009-09-01

    Full Text Available Background and objective Serum levels of tumor markers are associated with tumor metabolism or apoptosis, changes of which after chemotherapy may reflect tumor response to treatment. The aim of this study was to assess the predictive role of changes in serum levels of carcinoembryonic antigen (CEA and cytokeratin 19 fragment (CYFRA 21-1 during chemotherapy in patients with advanced non-small cell lung cancer. Methods Changes in serum levels of CEA and CYFRA 21-1 were investigated retrospectively after one cycle of chemotherapy in 42 patients with advanced NSCLC. Correlations between the changes and radiological objective response were analyzed. Results After two cycles of chemotherapy, radiological objective response rate was 28.6%. At baseline, gender, age, clinical stage, serum levels of CEA and CYFRA 21-1 were not different between patients with objective response (OR and no response (NR. After one cycle of chemotherapy, compared to baseline level, declines in serum levels of CEA and CYFRA 21-1 were observed in patients with OR, but have no statistical significance. In contrast, reduction of CEA and CYFRA 21-1 over baseline after one cycle of chemotherapy showed statistically significant difference between OR and NR. When reduction percentages of CEA and CYFRA 21-1 were used to predict objective response of chemotherapy, the area under the ROC curve (AUC was 0.875 for CEA and 0.919 for CYFRA 21-1. According to the ROC curve, a 22% reduction of CEA yielded a sensitivity of 58.3% and a specificity of 97%, 51% reduction of CYFRA 21-1 with a sensitivity of 83.3% and a specificity of 93.3%. When above reduction percentages were used as cutoffs for prediction of radiological objective response, combination of the CEA and CYFRA 21-1 yielded a sensitivity of 91.7% and a specificity of 86.7%. Conclusion Reduction percentages of CEA and CYFRA 21-1 during chemotherapy could be used to evaluate chemotherapy efficacy in patients with advanced NSCLC. The

  2. Correlation of Cyfra 21-1 levels in saliva and serum with CK19 mRNA expression in oral squamous cell carcinoma.

    Science.gov (United States)

    Malhotra, Rewa; Urs, Aadithya B; Chakravarti, Anita; Kumar, Suman; Gupta, V K; Mahajan, Bhawna

    2016-07-01

    Oral squamous cell carcinoma (OSCC) accounts for 90 % of malignant lesions of oral cavity. The study assessed the potential of Cyfra 21-1 as a tumor marker in OSCC. The study included 50 patients of OSCC to evaluate levels of Cyfra 21-1 in serum and saliva by electrochemiluminescent immunoassay (ECLIA) and CK19 messenger RNA (mRNA) expression in tissue by florescent quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) along with healthy individuals as control. The salivary and serum Cyfra 21-1 levels in patients of OSCC were significantly higher compared to controls (p value < 0.01). There was a 2.75-fold increase in CK19 mRNA expression in OSCC cases compared to controls. A significant positive correlation was found between serum and salivary Cyfra 21-1, serum Cyfra 21-1, and CK19 mRNA expression and between salivary Cyfra 21-1 and CK19 mRNA expression. Among these, correlation between serum and salivary Cyfra 21-1 was highly significant. Salivary and serum Cyfra 21-1 showed significantly elevated levels in grade II OSCC compared to grade I histopathologically. Elevated levels of salivary Cyfra 21-1 were associated with recurrence in OSCC patients. Reverse operating curve constructed using 3 ng/ml as a cutoff for serum Cyfra 21-1 revealed the sensitivity and specificity to be 88 and 78.2 %, respectively. Using a cutoff value of 8.5 ng/ml for salivary Cyfra 21-1, the sensitivity was found to be 93.8 % and specificity 84.3 %. We advocate salivary Cyfra 21-1 as a better diagnostic marker over serum Cyfra 21-1 as well as a potential marker in the prognosis of OSCC.

  3. Serum tumor marker CYFRA 21-1 in the diagnostics of squamous cell lung cancer - comparison with CEA

    International Nuclear Information System (INIS)

    Berzinec, P.; Letkovicova, M.; Arpasova, M.; Zuffova, H.

    1996-01-01

    The aim of the study was to test the diagnostic value tumor marker CYFRA 21-1 for squamous cell lung cancer (SQCLC) in comparison with carcinoembryonic antigen (CEA). Ninety-one patients were induced in this study: 56 with SQCLC - Group I, 25 with other types of lung cancer - Group II, 10 with benign respiratory tract diseases - Group III. Median CYFRA 21-1 serum concentration (ng/ml) was: in Group I: 4.52 (0.94 - >16), in Group II: 3.58 (1.72 - >16), in Group III: 2.05 (0.99 - 3.41). Median CEA serum concentration (ng/ml) was: in Group I: 4.49 (076- >20), in Group II: 3.32 (1.17 - >20), in Group III: 3.09 (1.84 - 6.37). There was a highly significant difference between the levels of CYFRA 21-1 in group I and Group III (p < 0.001), but there was no statistically significant difference between the levels of CEA in Group I and III. Sensitivity of CYFRA 21-1 by the cut-off 3.33 ng/ml in the diagnostics of SQCLC was 0.68, specificity 0.090, positive predictive value 0.91, negative predictive value 0.65. Sensitivity of CEA by cut-off 4.61 ng/ml was 0.5 by the same specificity 0.90. CYFRA 21-1 has high sensitivity, specificity and positive predictive value in the diagnostics of SQCLC. Sensitivity of CYFRA 21-1 is significantly higher than sensitivity of CEA in this settings. (author)

  4. Clinical usefulness of CEA, CA19-9, and CYFRA 21-1 as tumor markers for urothelial bladder carcinoma.

    Science.gov (United States)

    Washino, Satoshi; Hirai, Masaru; Matsuzaki, Atsushi; Kobayashi, Yutaka

    2011-01-01

    To evaluate the usefulness of measuring serum CEA, CA19-9, and CYFRA 21-1 levels for the diagnosis and monitoring of bladder cancer. Serum levels of CEA, CA19-9, and CYFRA 21-1 were measured in 85 patients with bladder cancer. The absolute level of each marker and the positive rate were compared with the clinical stage and histological grade of the tumor. Changes of the markers were assessed in patients with or without disease progression, and the correlations between survival and positivity/negativity of these markers were also evaluated. A higher serum level of CYFRA 21-1 was significantly correlated with higher tumor stage (p CEA and CA19-9 levels did not differ significantly among each stage and grade. The CYFRA 21-1 level increased significantly along with disease progression (from 7.33 ± 13.3 to 55.9 ± 127 ng/ml, p marker of advanced- and high-grade urothelial carcinoma of the bladder. It is useful for monitoring this disease and for predicting the prognosis. In contrast, the clinical usefulness of CEA and CA19-9 as tumor markers was not demonstrated. Copyright © 2011 S. Karger AG, Basel.

  5. Clinical significance of measurement of changes of serum IGF-II, EGF and CYFRA21-1 levels after chemotherapy in patients with lung cancer

    International Nuclear Information System (INIS)

    Chen Jianlin

    2010-01-01

    Objective: To study the clinical significance of changes of serum IGF-II, EGF and CYFRA21-1 levels after chemotherapy in patients with lung cancer. Methods: Serum IGF-II, EGF (with RIA), and CYFRA21-1 (with ECLIA) levels were determined both before and after chemotherapy in 39 patients with lung cancer as well as once in 35 controls. Results: Before chemotherapy, serum IGF-II, EGF and CYFRA21-1 levels were significantly higher in the patients than those in controls(P<0.01). Six months after chemotherapy, serum IGF-II, EGF and CYFRA21-1 levels dropped markedly, but remained significantly higher than those in controls(P<0.05). Conclusion: The development of lung cancer in patients was closely related to the serum IGF-II, EGF and CYFRA21-1 levels. (authors)

  6. Evaluation of a new tumor marker, CYFRA 21-1, in patients with primary lung cancer

    International Nuclear Information System (INIS)

    Yoshida, Tsuyoshi; Ichiya, Yuichi; Kuwabara, Yasuo

    1996-01-01

    We measured the level of serum CYFRA 21-1 (CYFRA) in 38 primary lung cancer patients (21 adenocarcinomas (ACs), 12 squamous cell carcinomas (SqCCs) and 5 small cell carcinomas (SmCCs)) before undergoing therapy using a 'CYFRA immunoradiometric assay kit'. We thus determined the CYFRA cut-off level to be 1.7 ng/ml. We also measured both the carcinoembryonic antigen (CEA), and the squamous cell carcinoma antigen (SCC) levels. The positive rate for CYFRA in patients with lung cancer was 71% and such positivity was related to their histology (SqCCs, SmCCs 100%; ACs 48%). The level of CYFRA was significantly higher in SqCCs than in either ACs or SmCCs. No significant differences were observed in the serum CYFRA value among the various clinical stages. In addition, no significant correlation was seen between CYFRA and SCC, or between CYFRA and CEA. Among the various clinical stages of SqCCs, the positive rate of CYFRA was higher in the early clinical stages than in SCC. Our findings therefore support the usefulness of CYFRA as a marker of SqCCs. (author)

  7. Study of a new tumor marker, CYFRA 21-1, in squamous cell carcinoma of the cervix, and comparison with squamous cell carcinoma antigen

    International Nuclear Information System (INIS)

    Tsai, S.Ch.; KAo, CH.H.; Wang, S.J.

    1996-01-01

    The diagnosis value of a new tumor marker, CYFRA 21-1, was studied in the blood samples collected from 22 controls, and 87 pre-treatment patients with squamous cell carcinoma of the cervix. Sensitivity and specificity of CYFRA 21-1 was was compared with those of squamous cell carcinoma antigen (SCC) measured in the sera of the same patients. Serum CYFRA 21-1 levels were higher in patients with squamous cell carcinoma than in controls (p < 0.05), and correlated with FIGO stage (Stage IIb-IV vs. Stage Ib-IIa, p = 0.0477). Using 2.5 ng/ml as cut-off value, elevated CYFRA 21-1 levels were found in 13.6% of controls, 34.8% of patients with Stage Ib-IIa squamous cell carcinoma of the cervix, and 63.5% of patients with Stage IIb-IV squamous cell carcinoma of the cervix. However, there was less sensitivity and specificity of CYFRA 21-1 than those of SCC in detecting squamous cell carcinoma of the cervix. CYFRA 21-1 may not be a better tumor marker than SCC for squamous cell carcinoma of the cervix. (author)

  8. Study on the relationship between serum concentration of CYFRA21-1 and pathological staging in patients with non-small cell lung cancer

    International Nuclear Information System (INIS)

    Shang Wenjun; Zhou Yaohong; Wang Xiaoli; Wu Yizhi; Li Jun

    2010-01-01

    Objective: To study the relationship between of serum concentrations of CYFRA21-1 and to pathological staging in patients with non-small cell lung cancer. Methods: Serum concentrations of CYFRA21-1 were determined with IRMA in 224 patients with non-small cell lung cancer. Results: The serum CYFRA21-1 levels in patients with non-small cell lung carcinoma increased gradually as the tumor size enlarged. Levels in patients of T2 and T3 stages were significantly higher than those in patients of T1 stage, but the difference between those in patients of T2 stage and T3 stage were not significant. The serum CYFRA21-1 levels also increased as the number of lymph nodes with metastasis increased. Differences of serum levels of CYFRA21-1 in patients of consecutive lymph node stages were all significant. Conclusion: Preoperative detection of the serum concentration of CYFRA21-1 in patient with non-small cell lung cancer has important clinical significance on the judgement of T, N stages. (authors)

  9. Measurements of free and total PSA, tissue polypeptide-specific antigen (TPS), and CYFRA 21-1 in prostate cancer patients under intermittent androgen suppression therapy.

    Science.gov (United States)

    Theyer, G; Dürer, A; Theyer, U; Haberl, I; Ulsperger, E; Baumgartner, G; Hamilton, G

    1999-10-01

    The present study evaluated monthly measurements of free and total prostate-specific antigen (PSA), and the tumor proliferation markers tissue polypeptide-specific antigen (TPS) and cytokeratin fragment 21-1 (CYFRA 21-1) in patients with advanced prostate cancer receiving intermittent androgen suppression therapy (IAS). Thirty-four men received alternating cycles of 8 month androgen suppression and treatment cessation (mean duration, 10.3 months) until PSA increased to >20 microg/l. Measurements of testosterone, percentage of free PSA, TPS, and CYFRA 21-1 were performed using ELISA and RIA assays. Periods of androgen suppression resulted in reversible reductions of testosterone (from 6 +/- 0.8 to IAS cycle. TPS showed a decrease of 50% after 3 months, and CYFRA 21-1 a 25% decrease after 7 months of androgen suppression treatment. During treatment cessation, TPS exceeded the normal cutoff value of 90 U/l late in tumor regrowth (9-11 months), whereas CYFRA 21-1 remained below the normal cutoff value of 3.3 ng/ml. PSA is the best and most sensitive marker of prostate cancer regression and regrowth during IAS cycles of the markers tested in this study. Free PSA constitutes approximately 15% of total PSA (range, 5-32%), and its percentage showed no significant change during IAS cycles. The TPS and CYFRA 21-1 proliferation marker changes in IAS seem to be related mainly to effects on normal androgen-dependent tissues. Copyright 1999 Wiley-Liss, Inc.

  10. Fine-needle aspirates CYFRA 21-1 is a useful tumor marker for detecting axillary lymph node metastasis in breast cancer patients.

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    Jung Hyun Yoon

    Full Text Available INTRODUCTION: To assess whether the value of CYFRA21-1 in the aspirates of ultrasonography-guided fine-needle aspiration biopsy (US-FNAB can contribute to improving the performances of US-FNAB in the diagnosis of axillary lymph node (LN metastasis in breast cancer patients. METHODS: US-FNAB was performed in 156 axillary LNs in 152 breast cancer patients (mean age: 51.4 years, range: 17-92 years. Concentrations of CYFRA21-1 were measured from washouts of the syringe used during US-FNAB. Tumor marker concentrations, US-FNAB, intraoperative sentinel node biopsy (SNB, and surgical pathology results were reviewed and analyzed. For comparison, the values of CEA and CA15-3 were also measured from washouts. RESULTS: Among the 156 LNs, 75 (48.1% were benign, and 81 (51.9% were metastases. Mean concentrations of CYFRA21-1 were significantly higher in metastasis compared to benign LNs (P<0.001. US-FNAB combined to CYFRA21-1 showed significantly higher sensitivity, NPV, and accuracy compared to US-FNAB alone (all values P<0.05. All diagnostic indices of US-FNAB combined to CYFRA21-1 were significantly higher compared to US-FNAB combined with CEA or CA15-3 (all P<0.001. Of the 28 metastatic LNs which showed metastasis on SNB, CYFRA21-1 showed higher positive rate of 75.0% (CEA or CA15-3∶60.7%, P = 0.076. CONCLUSION: Measuring CYFRA 21-1 concentrations from US-FNAB aspirates improves sensitivity, NPV, and accuracy of US-FNAB alone, and may contribute to reducing up to 75.0% of unnecessary intraoperative SNB. Compared to CEA or CA15-3, CYFRA21-1 shows significantly higher performances when combined to US-FNAB in the preoperative diagnosis of LN metastasis in breast cancer patients.

  11. The value of combined examination of serum CYFRA21-1 levels and bone scan in the diagnosis of bone metastasis in lung cancer

    International Nuclear Information System (INIS)

    Yu Jing; Wang Junhong; Zhengping

    2007-01-01

    Objective: To explore the value of combined examination of serum tumor markers CYFRA21-1 and bone scan in the diagnosis of bone metastasis in lung cancer. Methods: Bone scan and serum CYFRA21-1 levels (with CLIA) determination were performed in 138 patients with lung cancer and 56 patients with benign lung diseases. Results: The serum level of CYFRA21-1 were significantly higher in patients with bone metastasis than those in patients without bone metastasis. The levels were also higher in patients without bone metastasis than those in controls. Most patients with bone metastasis had positive results in bone scan (97.4%), only 2 of the 78 had negative bone scan but positive with CT or MRI. A few patients without bone metastasis and controls had positive bone scan results, caused by previous operation or injury. Conclusion: The combined detection of CYFRA21-1 and bone scan were valuable in the diagnosis of bone metastasis of lung cancer. (authors)

  12. Clinical significance of measurement of changes of serum IGF-II, SCC and CYFRA21-1 levels after operation in patients with carcinoma of uterine cervix

    International Nuclear Information System (INIS)

    Kuang Lei

    2010-01-01

    Objective: To explore the clinical significance of changes of serum IGF-II, SCC and CYFRA21-1 levels after operation in patients with carcinoma uterine cervix. Methods: Serum levels of IGF-II, SCC and CYFRA21-1 were determined with RIA repeatedly in 31 patients with carcinoma of uterine cervix (before operation 1 month after operation and 6 month after operation) and once in 35 controls. Results: Before operation,serum levels of IGF-II, SCC and CYFRA21-1 in the patients were significantly higher than those in the controls (P<0.01). One month after operation all the serum levels were approaching normal. Six month later,the levels in the patients without recurrence remained normal. However, the levels in the 6 patients with recurrence returned to those before operation again. Conclusion: Changes of serum IGF-II, SCC and CYFRA21-1 levels are closely related to the tumor burden and may be of prognostic importance. (authors)

  13. A sandwich-type electrochemical immunoassay for ultrasensitive detection of non-small cell lung cancer biomarker CYFRA21-1.

    Science.gov (United States)

    Zeng, Yan; Bao, Jing; Zhao, Yanan; Huo, Danqun; Chen, Mei; Qi, Yanli; Yang, Mei; Fa, Huanbao; Hou, Changjun

    2018-04-01

    Many studies confirm that the aberrant expression of Cytokeratin 19 fragment 21-1 (CYFRA21-1) is highly correlated with non-small cell lung cancer (NSCLC), especially for squamous cell carcinoma. Herein, we report a sandwich-type electrochemical immunosensor based on signal amplification strategy of multiple nanocomposites to test CYFRA21-1 selectively and sensitively. The proposed immunosensor fabricated by three-dimensional graphene (3D-G), chitosan (CS) and glutaraldehyde (GA) composite on the glass carbon electrode (GCE) with a large surface area is prepared to immobilize primary antibodies (Ab 1 ) and provide excellent conductivity. To further amplify the electrochemical signal, the trace tag on the foundation of gold nanoparticles (AuNPs) is coated with amino-functionalized carbon nanotube (MWCNT-NH 2 ) nanocomposite through thionine linking, which provides more amino groups to capture more horseradish peroxidase-labeled antibodies (HPR-Ab 2 ) and enhances the conductivity. Under optimal conditions, the developed immunosensor exhibits excellent analytical performance for the determination of CYFRA21-1 with a wide linear range from 0.1 to 150ng·mL -1 and a low detection limit (LOD) of 43pg·mL -1 . Furthermore, satisfactory results are obtained for the determination of CYFRA21-1 in real clinical serum samples, indicating the potential of the immunoassay to be applied in clinical analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma.

    Science.gov (United States)

    Yuan, Chuanshu; Yang, Kai; Tang, Hong; Chen, Dan

    2016-01-01

    At present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1), squamous cell carcinoma antigen (SCCAg), ferritin, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and α-fetoprotein (AFP) for patients with oral/oropharyngeal squamous carcinoma (OSCC/OPSCC). One hundred and sixty-nine cases of patients with OSCC/OPSCC as the experimental group, 86 cases of oral benign tumor patients as the control group, and 30 cases of healthy people as the normal control group were studied. The levels of serum Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP were measured using electrochemiluminescence immunoassay. The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in patients with OSCC/OPSCC were significantly higher than those of benign tumor and healthy control group (Ptumor, and healthy group (P>0.05). The level of serum Cyfra21-1 in patients with early OSCC/OPSCC (stage I + II) was significantly higher than that of benign tumor and healthy control group (PCEA, CA19-9, and AFP showed no significant difference between patients with early OSCC/OPSCC, benign tumor, and healthy control group (P>0.05). The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in the middle-late stage of patients with OSCC/OPSCC (stage III + IV) were significantly higher than those of patients with the early OSCC/OPSCC, benign tumor, and healthy control group (PCEA were 2.17, 0.72, 109.95, and 1.99 ng/mL, respectively. The sensitivities were 60.36%, 73.37%, 81.66%, and 66.27%, respectively. The specificities were 81.03%, 68.10%, 40.52%, and 61.21%, respectively. Cyfra21-1, SCCAg, ferritin, and CEA had diagnostic values for patients with OSCC/OPSCC. Meanwhile, Cyfra21-1 had better early diagnostic value for patients with OSCC/OPSCC.

  15. The CT Findings and the Peak SUV on PET/CT according to the Levels of Cyfra 21-1 and CEA in Patients with Non-Small Cell Lung Carcinoma

    International Nuclear Information System (INIS)

    Jou, Sung Shick; Kim, Young Tong; Han, Jong Kyu; Kim, Hyung Hwan; Park, Jeong Mi; Park, Jai Soung; Hwang, Jung Hwa

    2009-01-01

    We wanted to evaluate the CT findings and the peak SUV according to the levels of cyfra 21-1 and CEA in patients with non-small cell lung carcinoma (NSCLC). We evaluated the TNM staging, cell types, the CT findings and peak SUV of the NSCLC in 234 patients with NSCLC according to the tumor marker levels. The correlations of the CT findings and the peak SUV with the tumor markers were evaluated in 35 patients with stage I disease. The sensitivities of the combined tumor markers cyfra 21-1 and CEA in the NSCLC for each TNM staging (I-IV) were 48.5%, 66.7%, 78.3% and 84.3%, respectively (p<0.05). Cyfra 21-1 was more sensitive for squamous cell carcinoma and CEA was more sensitive for adenocarcinoma. The tumor size, tumor necrosis and peak SUV were greater in the NSCLC with an elevated cyfra 21-1 level than that in the NSCLC without an elevated cyfra 21-1 level (p<0.05). For stage I disease, the level of cyfra 21-1 was linearly correlated with the tumor size (r=0.54) and the peak SUV (r=0.46), and the level of CEA was high in the spiculated masses (p<0.05). The NSCLC with an elevated cyfra 21-1 level shows larger, more frequently necrosis and a higher peak SUV than the NSCLC without an elevated Cyfra 21-1 level. For stage 1 disease, the tumor size and peak SUV correlate with the level of cyfra 21-1, and spiculated masses show an elevated level of CEA

  16. The CT Findings and the Peak SUV on PET/CT according to the Levels of Cyfra 21-1 and CEA in Patients with Non-Small Cell Lung Carcinoma

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    Jou, Sung Shick; Kim, Young Tong; Han, Jong Kyu; Kim, Hyung Hwan [Soonchunhyang University Cheonan Hospital, Cheonan (Korea, Republic of); Park, Jeong Mi; Park, Jai Soung [Soonchunhyang University Bucheon Hospital, Bucheon (Korea, Republic of); Hwang, Jung Hwa [Soonchunhyang University Hospital, Seoul (Korea, Republic of)

    2009-10-15

    We wanted to evaluate the CT findings and the peak SUV according to the levels of cyfra 21-1 and CEA in patients with non-small cell lung carcinoma (NSCLC). We evaluated the TNM staging, cell types, the CT findings and peak SUV of the NSCLC in 234 patients with NSCLC according to the tumor marker levels. The correlations of the CT findings and the peak SUV with the tumor markers were evaluated in 35 patients with stage I disease. The sensitivities of the combined tumor markers cyfra 21-1 and CEA in the NSCLC for each TNM staging (I-IV) were 48.5%, 66.7%, 78.3% and 84.3%, respectively (p<0.05). Cyfra 21-1 was more sensitive for squamous cell carcinoma and CEA was more sensitive for adenocarcinoma. The tumor size, tumor necrosis and peak SUV were greater in the NSCLC with an elevated cyfra 21-1 level than that in the NSCLC without an elevated cyfra 21-1 level (p<0.05). For stage I disease, the level of cyfra 21-1 was linearly correlated with the tumor size (r=0.54) and the peak SUV (r=0.46), and the level of CEA was high in the spiculated masses (p<0.05). The NSCLC with an elevated cyfra 21-1 level shows larger, more frequently necrosis and a higher peak SUV than the NSCLC without an elevated Cyfra 21-1 level. For stage 1 disease, the tumor size and peak SUV correlate with the level of cyfra 21-1, and spiculated masses show an elevated level of CEA.

  17. Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Yuan CS

    2016-06-01

    Full Text Available Chuanshu Yuan, Kai Yang, Hong Tang, Dan Chen Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China Background: At present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1, squamous cell carcinoma antigen (SCCAg, ferritin, carcinoembryonic antigen (CEA, carbohydrate antigen 19-9 (CA19-9, and α-fetoprotein (AFP for patients with oral/oropharyngeal squamous carcinoma (OSCC/OPSCC. Methods: One hundred and sixty-nine cases of patients with OSCC/OPSCC as the experimental group, 86 cases of oral benign tumor patients as the control group, and 30 cases of healthy people as the normal control group were studied. The levels of serum Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP were measured using electrochemiluminescence immunoassay. Results: The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in patients with OSCC/OPSCC were significantly higher than those of benign tumor and healthy control group (P<0.05. The levels of CA19-9 and AFP showed no significant difference between patients with OSCC/OPSCC, benign tumor, and healthy group (P>0.05. The level of serum Cyfra21-1 in patients with early OSCC/OPSCC (stage I + II was significantly higher than that of benign tumor and healthy control group (P<0.05. However, the levels of serum SCCAg, ferritin, CEA, CA19-9, and AFP showed no significant difference between patients with early OSCC/OPSCC, benign tumor, and healthy control group (P>0.05. The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in the middle-late stage of patients with OSCC/OPSCC (stage III + IV were significantly higher than those of patients with the early OSCC/OPSCC, benign tumor, and healthy control group (P<0.05. The diagnostic cutoff levels of Cyfra21-1

  18. Avaliação dos valores sérico e pleural dos marcadores tumorais CEA, CYFRA21-1 e CA 15-3 em portadores de derrame pleural Evaluation of serum and pleural levels of the tumor markers CEA, CYFRA21-1 and CA 15-3 in patients with pleural effusion

    Directory of Open Access Journals (Sweden)

    Isabella Coimbra Wagner

    2007-04-01

    Full Text Available OBJETIVO: Dosar os marcadores tumorais antígeno carcinoembrionário (CEA, fragmento da citoqueratina 19 (CYFRA21-1 e antígeno glicosídico associado a tumor 15-3 (CA 15-3 em sangue e líquido pleural de portadores de derrames pleurais benignos e malignos, avaliando a sensibilidade de cada um deles nesses fluidos. MÉTODOS: Avaliamos prospectivamente 85 pacientes com derrame pleural. O estudo do líquido pleural obedeceu a critérios determinados pela literatura. A dosagem dos marcadores foi realizada por eletroquimioluminescência. A sensibilidade foi determinada sob a condição de que a especificidade fosse > 90%. RESULTADOS: Foram diagnosticados 36 casos malignos (42,4%, 30 benignos (35,3%; em 19 pacientes (22,3%, o diagnóstico foi inconclusivo. Nos casos malignos, os valores de CEA e CYFRA21-1 foram maiores no líquido pleural do que no sangue, fato não observado para o CA 15-3. Nos casos benignos, os valores do CYFRA21-1 foram maiores no líquido pleural do que no soro, enquanto que para o CEA e o CA 15-3, ocorreu o oposto. Todos os marcadores apresentaram diferença significativa entre os casos malignos e benignos, em líquido pleural e soro. Foi encontrada sensibilidade para CEA, CYFRA21-1 e CA 15-3 no líquido pleural de 69,4%, 69,4% e 66,7%, respectivamente e quando associados, foi 80,6%. No soro, a sensibilidade foi 57,1, 71,4 e 48,6% para CEA, CYFRA21-1 e CA 15-3, respectivamente, e quando associados, foi 77%. CONCLUSÃO: Os resultados sugerem que a utilização desses marcadores pode ser útil na diferenciação entre derrames pleurais malignos e benignos.OBJECTIVE: To determine the levels of the tumor markers carcinoembryonic antigen (CEA, cytokeratin 19 fragment (CYFRA21-1 and carbohydrate antigen 15-3 (CA 15-3 in the blood and pleural fluid of patients with benign or malignant pleural effusion, evaluating the sensitivity of each marker in these fluids. METHODS: We prospectively evaluated 85 patients with pleural effusion. The

  19. Serum tumor markers CEA, CYFRA21-1, and CA-125 are associated with worse prognosis in advanced non-small-cell lung cancer (NSCLC).

    Science.gov (United States)

    Cedrés, Susana; Nuñez, Isaac; Longo, Marina; Martinez, Pablo; Checa, Eva; Torrejón, Davis; Felip, Enriqueta

    2011-05-01

    Serum tumor markers are considered a negative prognostic factor in early-stages NSCLC but its role in advanced disease is controversial. The aim of this study is to analyze the prognostic value of tumor markers in advanced NSCLC. Two hundred and seventy seven patients diagnosed in our institution were retrospectively reviewed. Baseline prognostic factors analyzed were gender, histology and brain metastases. Baseline patients characteristics: median age 63 years (30-81 years); males 84.4%, stage IV: 61.7%; adenocarcinoma 38.6%, squamous carcinoma 22.4%. High levels of CEA, CYFRA21-1, and CA125 levels were detected in 179 (55.9%), 119 (65%), and 129 (46.6%) patients respectively. Significant higher levels of CEA and CA125 at baseline were present in adenocarcinoma (P CEA, CYFRA21-1, and CA125 was 5.3 months (m), 3.5 m and 4.6 m versus 7.4 m, 6.2 m and 7.5 m in patients with normal levels (P tumor markers was 10.0 m vs 14.0 m (P = 0.085) for CEA; 5.6 vs 12.1 m for CYFRA21-1 (P = .002), and 8.7 vs 14.0 (P = .03) for CA125. In the multivariate analysis high levels of tumor markers, histology and clinical stage were significant correlated with worse prognostic. Patients with all the tumor markers elevated presented the worst prognosis (3.6 m for PFS and 7.1 m for OS, P tumor markers at baseline are correlated with worse survival in stage III-IV NSCLC patients. Copyright © 2011 Elsevier Inc. All rights reserved.

  20. The Diagnostic and Prognostic Value of Tumor Markers (CEA, SCC, CYFRA 21-1, TPS) in Head and Neck Cancer Patients.

    Science.gov (United States)

    Barak, Vivian; Meirovitz, Amichay; Leibovici, Vera; Rachmut, Jacob; Peretz, Tamar; Eliashar, Ron; Gross, Menachem

    2015-10-01

    Establishing prognostic factors is very important in the management of cancer patients. Our aim was to evaluate the clinical significance of a panel of tumor markers, including CEA (Carcino Embryonic Antigen), SCC (Squamous Cell Carcinoma Antigen), TPS (Tissue Polypeptide Specific Antigen) and CYFRA 21-1 in head and neck cancer patients, for assessing treatment response and prognosis of patients. We evaluated 312 blood samples from 143 head and neck cancer patients, from several sub-groups: 82 Larynx Carcinoma pre- and 38 post-therapy, 46 Oral Cavity pre and 29 post-therapy, 12 nasopharynx, 16 parotid and other salivary gland patients. Blood tumor markers levels were evaluated by conventional ELISA assays. Correlations of marker levels to stage of disease, lymph node involvement and therapy, were performed. Serum levels of all four tumor markers were higher before therapy and decreased thereafter in all patients. The decrease in TPS level following therapy was significant (p=0.03). Significantly higher levels of TPS and similarly higher levels of the other tumor markers were demonstrated in advanced disease (stages III and IV) patients, as opposed to early disease (stages I and II) patients (p=0.012). Node positive patients had significantly higher TPS levels as compared to node negative (p=0.02). The same trend was shown by the other markers as well, but did not reach statistical significance. TPS was best correlated to survival of patients; those having low levels had the best clinical outcome and longer survival. CEA, SCC, TPS and CYFRA 21-1 can all serve as useful tumor markers in HNC patients. They assessed response to therapy and were prognostic for recurrence. TPS proved to be the most sensitive predictor of advanced disease and poor prognosis. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  1. Effect of endostar combined with paclitaxel liposome and radiotherapy simultaneously on serum CYFRA21-1, CEA, SCCA, CA125, IL-8 and T lymphocyte subsets in patients with advanced cervical cancer

    Directory of Open Access Journals (Sweden)

    Wen-Jing Yang

    2016-08-01

    Full Text Available Objective: To study the effect of endostar combined with paclitaxel liposome and radiotherapy simultaneously on serum CYFRA21-1, CEA, SCCA, CA125, IL-8 and T lymphocyte subsets in patients with advanced cervical cancer. Methods: A total of 72 patients with advanced cervical cancer in our hospital from January 2014 to February 2016 were enrolled in this study. The subjects were divided into control group (n=36 and experiment group (n=36 randomly. The control group were treated with radiotherapy, the experiment group were treated with endostar combined with paclitaxel liposome and radiotherapy simultaneously. 3 weeks for a period of treatment and the two groups were treated for 4 periods. The serum CYFRA21-1, CEA, SCCA, CA125, IL-8 levels and peripheral blood CD3+, CD4+ and CD8+ cells of the two groups before and after treatment were compared. Results: There were no significantly differences of the serum CYFRA21-1, CEA, SCCA, CA125, IL-8 levels and peripheral blood CD3+, CD4+ and CD8+ cells of the two groups before treatment. The serum CYFRA21-1, CEA, SCCA, CA125 and IL-8 levels of the two groups after treatment were significantly lower than before treatment, and that of experiment were significantly lower than control group. The peripheral blood CD3+, CD4+ cells of the two groups after treatment were significantly lower than before treatment, CD8+ cells of the two groups after treatment were significantly higher than before treatment, and that of experiment group were significantly better than control group. Conclusion: Endostar combined with paclitaxel liposome and radiotherapy simultaneously can significantly reduce the serum CYFRA21-1, CEA, SCCA, CA125 and IL-8 levels, improve peripheral blood CD3+, CD4+ and CD8+ levels of patients with advanced cervical cancer, and it was worthy clinical application.

  2. Performance characteristics of SCC radioimmunoassay and clinical significance serum SCC Ag assay in patients with malignancy

    International Nuclear Information System (INIS)

    Kim, Dong Youn

    1986-01-01

    To evaluate the performance characteristics of SCC RIV and the clinical significance of serum SCC Ag assay in patients with malignancy, serum SCC Ag levels were measured by SCC RIV kit in 40 normal controls and 35 percents with various untreated malignancy, who visited Chonju Presbyterian Medical Center. The results were as follows; 1. The SCC RIA was simple to perform and can be completed in two workday. And the standard curve and reproducibility were both good. 2. The mean serum SCC Ag level in normal controls was 1.64 ± 0.93 ng/mL and normal upper limit of serum SCC Ag was defined as 2.6 ng/mL. 3 out of 40 (7.5%) normal controls showed elevated SCC Ag levels above the normal upper limit. 3. In 35 patients with various untreated malignancy, 18 patients (51.4%) showed elevated serum SCC Ag levels, 59.1% of 22 patients with cervical cancer, 80% of 5 patients with lung cancer, 33% of 3 patients with esophageal cancer, 0% of 2 patients with rectal cancer and 0% of 3 patients with breast cancer showed elevated serum SCC Ag levels. Above results represent that SCC RIV is simple method to perform followed by good standard curve and reproducibility, and may be a useful indicator reflecting diagnostic data of patients with cervical cancer and lung cancer

  3. O Cyfra 21-1 é um determinante prognóstico no carcinoma pulmonar não de pequenas células (CPNPC: resultado de uma meta-análise que incluiu 2063 doentes

    Directory of Open Access Journals (Sweden)

    J.-L. Pujol

    2004-11-01

    de prognóstico isolado 4.Por outo lado, numa revisão de 500 trabalhos publicados 5, defende-se que os marcadores tumorais não têm lugar na avaliação prognóstica dos doentes com CPNPC, o que não está de acordo com outros que afirmam que o Cyfra 21-1 se correlaciona bem com o TNM e o PS, constituindo um factor de prognóstico independente, e recomendando-o, mesmo, como uma co-variável a incluir em futuros ensaios clínicos 6.A meta-análise que apresentámos vem reforçar a opinião de que os valores séricos pré-terapêuticos elevados de Cyfra 21-1, nos CPNPC, constituem um factor de prognóstico isolado, independentemente do estádio da doença e da terapêutica instituída, reforçando as conclusões de outro publicado recentemente 7, em que esse marcador tumoral se revelou também nesse grupo de tumores, em estádios IIIB/IV, como um factor de prognóstico, quer isoladamente, quer quando associado a dois outros, o antigénio carcinoembrionário (CEA e a neuroenolase específica (NSE.Nos doente cirúrgicos, o valor pré-terapêutico elevado de Cyfra 21-1, eventualmente, poderá indicar a necessidade de quimioterapia adjuvante, o que poderá ser mais um argumento a reforçar as conclusões do projecto IALT 8, em que se defende que aquela abordagem terapêutica poderá ser útil, também, nos estádios mais localizados da doença.

  4. Performance of biomarkers SMRP, CA125, and CYFRA 21-1 as potential tumor markers for malignant mesothelioma and lung cancer in a cohort of workers formerly exposed to asbestos

    International Nuclear Information System (INIS)

    Gube, M.; Taeger, D.; Weber, D.G.; Pesch, B.; Johnen, G.; Gross, I.M.; Wiethege, T.; Weber, A.; Bruening, T.; Brand, P.; Mueller-Lux, A.; Kraus, T.; Raithel, H.J.

    2011-01-01

    The aim of the study is to examine the cancer-predictive values of SMRP (soluble mesothelin-related peptides), CA125, and CYFRA21-1 as potential tumor markers for lung cancer and malignant mesothelioma in a cohort of workers formerly exposed to asbestos. A voluntary surveillance program has been established for German workers with former asbestos exposure. A subgroup of 626 subjects with a mean age of 63 years (range 53-70 years) at baseline was enrolled in an extended health examination program with high-resolution computer tomography (HRCT) of the chest and blood drawing between 1993 and 1997. Serum concentrations of SMRP, CA125, and CYFRA21-1 were measured in archived serum samples in 2005 and 2006. A mortality follow-up was conducted through 2007. So far, 12 cases with lung cancer and 20 cases with malignant mesothelioma have been observed in this cohort. The average time between sample collection and diagnosis was 4.7 years. Analyzed biomarkers showed low sensitivities (5-25%) and positive predictive values (4-30%) for both cancer sites. Marker combinations resulted in sensitivities between 5 and 50% and positive predictive values ranging from 3 to 14%. Even in those cases, where biomarker concentrations were available within 36 months before diagnosis, no trend for increasing biomarker levels was observed. The analyzed tumor markers were characterized by high specificities, but low sensitivities. SMRP, CA125, and CYFRA21-1 alone or in combination were less suitable to serve as predictors for the diagnosis of lung cancer or malignant mesothelioma. However, a prospective study with annual sampling might reveal a better predictive value of these markers. (orig.)

  5. Performance of biomarkers SMRP, CA125, and CYFRA 21-1 as potential tumor markers for malignant mesothelioma and lung cancer in a cohort of workers formerly exposed to asbestos

    Energy Technology Data Exchange (ETDEWEB)

    Gube, M. [RWTH Aachen University, Institute for Occupational and Social Medicine, Medical Faculty, Aachen (Germany); Westfaelische Technische Hochschule, Institut fuer Arbeitsmedizin und Sozialmedizin am Universitaetsklinikum Aachen, Rheinisch, Aachen (Germany); Taeger, D.; Weber, D.G.; Pesch, B.; Johnen, G.; Gross, I.M.; Wiethege, T.; Weber, A.; Bruening, T. [Institute of the Ruhr-Universitaet Bochum (IPA), Institute for Prevention and Occupational Medicine of the German Social Accident Insurance, Bochum (Germany); Brand, P.; Mueller-Lux, A.; Kraus, T. [RWTH Aachen University, Institute for Occupational and Social Medicine, Medical Faculty, Aachen (Germany); Raithel, H.J. [University of Erlangen-Nuremberg, Institute for Occupational, Social and Environmental Medicine, Erlangen-Nuremberg (Germany)

    2011-03-15

    The aim of the study is to examine the cancer-predictive values of SMRP (soluble mesothelin-related peptides), CA125, and CYFRA21-1 as potential tumor markers for lung cancer and malignant mesothelioma in a cohort of workers formerly exposed to asbestos. A voluntary surveillance program has been established for German workers with former asbestos exposure. A subgroup of 626 subjects with a mean age of 63 years (range 53-70 years) at baseline was enrolled in an extended health examination program with high-resolution computer tomography (HRCT) of the chest and blood drawing between 1993 and 1997. Serum concentrations of SMRP, CA125, and CYFRA21-1 were measured in archived serum samples in 2005 and 2006. A mortality follow-up was conducted through 2007. So far, 12 cases with lung cancer and 20 cases with malignant mesothelioma have been observed in this cohort. The average time between sample collection and diagnosis was 4.7 years. Analyzed biomarkers showed low sensitivities (5-25%) and positive predictive values (4-30%) for both cancer sites. Marker combinations resulted in sensitivities between 5 and 50% and positive predictive values ranging from 3 to 14%. Even in those cases, where biomarker concentrations were available within 36 months before diagnosis, no trend for increasing biomarker levels was observed. The analyzed tumor markers were characterized by high specificities, but low sensitivities. SMRP, CA125, and CYFRA21-1 alone or in combination were less suitable to serve as predictors for the diagnosis of lung cancer or malignant mesothelioma. However, a prospective study with annual sampling might reveal a better predictive value of these markers. (orig.)

  6. Application of tumor markers SCC-Ag, CEA, and TPA in patients with cervical precancerous lesions.

    Science.gov (United States)

    Farzaneh, Farah; Shahghassempour, Shapour; Noshine, Bahram; Arab, Maliheh; Yaseri, Mehdi; Rafizadeh, Mitra; Alizadeh, Kamyab

    2014-01-01

    To determine the potential clinical utility of tumor markers CEA, TPA, and SCC-Ag for early detection of cervical precancerous lesions. A case-control study was carried out on 120 women (46 patients with histologically confirmed cervical precancerous lesions and 74 healthy controls). The significance of serum selected tumor markers in early detection of cervical intraepithelial neoplasia (CIN) were assessed. Of the case group, the rates of CIN I, II, III, was 69.6%, 23.9%, and 6.5%, respectively. According to the manufacturer's cut-off values of 2 ng/ml, 5 ng/ml, and 70 U/ml for SCC-Ag, CEA and TPA tests, in that order, SCC-Ag test had a sensitivity of 13%, but CEA and TPA tests could not distinguish between case and control groups. The diagnostic sensitivities were highest at cut-off values of 0.55 ng/ml for SCC-Ag, 2.6 ng/ ml for CEA, and 25.5 U/ml for TPA which were 93%, 61%, and 50%, respectively. However, the area under the receiver operating characteristic curve was the largest for SCC-Ag (0.95 vs. 0.61 and 0.60 for CEA and TPA, respectively). Moreover, there was a highly significant direct correlation between SCC-Ag concentration and the degree of cervical precancerous lesions (r=0.847, ptumor marker in Iranian patients with CIN and it needs to be more evaluated by studies with larger populationa.

  7. Tumor markers in the early detection of tumor recurrence in breast cancer patients: CA 125, CYFRA 21-1, HER2 shed antigen, LDH and CRP in combination with CEA and CA 15-3.

    Science.gov (United States)

    Di Gioia, Dorit; Blankenburg, Irene; Nagel, Dorothea; Heinemann, Volker; Stieber, Petra

    2016-10-01

    Kinetics of CA 15-3 and CEA have a high specificity in the early detection of metastatic breast cancer (MBC). However, this high specificity is associated with a lack of sensitivity. To decrease the number of false negative patients, the additional diagnostic potential of an extended panel of biomarkers was evaluated. This analysis was performed as part of a large follow-up study (1998-2010) evaluating 813 patients with a median follow-up of 63months. After primary therapy, all patients underwent tumor marker monitoring for CEA and CA 15-3 at 6-week intervals. A reproducible previously defined increase (≥100%) based on the individual baseline value of each patient was considered as a strong indicator of MBC. For the present analysis, we retrospectively evaluated 1011 blood samples from 95 patients. Forty-seven of these had metastatic disease for the first time at the time of this evaluation, while the remaining 48 patients showed no evidence of disease. The sera of these patients were additionally assessed for the following parameters: cancer antigen (CA) 125, cytokeratin-19 soluble fragment (CYFRA 21-1), HER2 shed antigen, lactate-dehydrogenase (LDH) and C-reactive protein (CRP). 26 of 47 patients with MBC showed a reproducible tumor marker increase of at least CEA and/or CA 15-3 (55.3%, true-positive). The remaining 21 patients with MBC showed no increase in CEA or CA 15-3 (44.7%, false negative, FN). By combining all markers mentioned above, 41 of 47 patients with MBC showed a reproducible marker increase with a sensitivity of 87.2% and specificity of 100%. This retrospective analysis indicates that a panel of biomarkers can increase the sensitivity of the CA 15-3/CEA combination without loss of specificity. The combined use is therefore helpful for early detection of MBC. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. The clinical significance of serum SCC-Ag combined with CD105 in patients with cervical cancer during the early stage diagnosis

    Directory of Open Access Journals (Sweden)

    Ru-Chan Ma

    2016-09-01

    Full Text Available Objective: To invest the clinical significance of serum SCC-Ag combined with CD105 in early diagnosis of cervical cancer to provide new ideas for early diagnosis and clinical treatment of cervical cancer. Methods: A total of 74 cases cervical cancer patients were selected as cervical cancer group, and 52 cases uterine fibroids patients were selected as normal cervical group, serum samples were collected in the early morning fasting condition, SCC-Ag and CD105 were checked by ELISA method, SCC-Ag and CD105 of two groups were analyzed by t-test, and to compare SCC-Ag and CD105 in different TMN staging, lymph gland metastasis and non-lymph gland metastasis in patients with cervical cancer, the correlation analysis was used by Pearson correlation analysis method. Results: These results came from ELISA method, comparing with normal cervical group, the SCC-Ag and CD105 of cervical cancer group increased, the difference was statistically significant. Comparing with Ⅰ period of TMN staging, SCC-Ag and CD105 of Ⅱ period increased, Ⅲ, Ⅳ period increased, the difference was statistically significant. Comparing with Ⅱ period, SCC-Ag and CD105 of Ⅲ, Ⅳ period increased, the difference was statistically significant. Comparing with non-lymph gland metastasis, SCC-Ag and CD105 of lymph gland metastasis increased in cervical cancer with surgical treatment, the difference was statistically significant. According to Pearson correlation analysis, SCC-Ag and CD105 were positively correlated. Conclusion: SCC-Ag and CD105 in patients with cervical cancer increase highly, it has important clinical value that of serum SCCAg combined with CD105 in the early diagnosis of cervical cancer, especially it has clinical guiding significance to staging and lymph gland metastasis of cervical cancer, and it is worthy of clinical reference.

  9. Introduction to NSE spectroscopy

    International Nuclear Information System (INIS)

    Pappas, C.

    2001-01-01

    Neutron Spin Echo (NSE) spectroscopy allows for reaching the highest energy resolution in inelastic neutron scattering while keeping the high intensity advantage of a beam which is only 10-20% monochromatic. Most spectroscopic methods determine separately the energies of the incident (ω 0 ) and scattered beams (ω) in order to deduce the energy transfer (Δω = ω-ω 0 ), which is the relevant parameter in inelastic neutron scattering. The accuracy in the determination of ω 0 and ω also determines the lowest limit for Δω, which can reach 10 -3 , but with the cost of a high incident beam monocromatisation. In NSE the precession of neutron spins in a magnetic field is used as a stop-watch, which is carried by each neutron individually and measures directly, with an accuracy of 10 -5 to 10 -3 , the difference in energy before and after the scattering process at the sample. (R.P.)

  10. NSE

    African Journals Online (AJOL)

    NESG PUBLICATIONS

    of women. Eliminate gender ... good governance, development and poverty reduction – nationally and ... Equality and Empower Women. The ratio of ... better household practices, access to drinking .... four-fifth of workforce are employed),.

  11. J-NSE: Neutron spin echo spectrometer

    Directory of Open Access Journals (Sweden)

    Olaf Holderer

    2015-08-01

    Full Text Available Neutron Spin-Echo (NSE spectroscopy is well known as the only neutron scattering technique that achieves energy resolution of several neV. By using the spin precession of polarized neutrons in magnetic field one can measure tiny velocity changes of the individual neutron during the scattering process. Contrary to other inelastic neutron scattering techniques, NSE measures the intermediate scattering function S(Q,t in reciprocal space and time directly. The Neutron Spin-Echo spectrometer J-NSE, operated by JCNS, Forschungszentrum Jülich at the Heinz Maier-Leibnitz Zentrum (MLZ in Garching, covers a time range (2 ps to 200 ns on length scales accessible by small angle scattering technique. Along with conventional NSE spectroscopy that allows bulk measurements in transmission mode, J-NSE offers a new possibility - gracing incidence spin echo spectroscopy (GINSENS, developed to be used as "push-button" option in order to resolve the depth dependent near surface dynamics.

  12. Serum CYFRA 21-1 in Egyptian women with breast cancer

    African Journals Online (AJOL)

    Samia A. Ebied

    2016-03-07

    Mar 7, 2016 ... associated with tumor size, clinical stage and axillary lymph node involvement. Serum ... primary treatment or in monitoring responses to treatment.4 ... small cell lung cancer10,11 has been reported to be detected in.

  13. A retrospective analysis of serum tumor markers found in non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    Qin Hu

    2016-01-01

    Conclusion: Although the positive CEA, CYFRA 21-1, NSE, and TSGF rates were observed at low values during the NSLCLC serum diagnosis, they still played an important role in diagnosing lung cancer. Significant levels of CEA, CYFRA 21-1, NSE, and TSGF were detected in the serum. The amounts found were useful for diagnosing NSCLC patients who depended on the currently limited biomarker development.

  14. Going to the limits of NSE

    International Nuclear Information System (INIS)

    Pappas, C.; Mezei, F.; Triolo, A.; Zorn, R.

    2005-01-01

    Neutron spin echo (NSE) is characterized by a broad variety of new developments. The obvious trend is towards the highest possible energy resolution at the highest possible wavelengths by further developing the generic IN11 design. The planned NSE spectrometer at the SNS will further push the limits of NSE reaching Fourier times as long as 1 μs. On the other hand, simultaneous measurements over higher total solid angles require the real cylindrical symmetry of the magnetic field configuration realized with the spectrometer SPAN at BENSC. The state-of-the-art neutron optical elements lead to considerable gains in neutron flux and allow for extending the wavelength range up to very high wavelengths as well as down to the thermal spectrum. With thermal neutrons NSE reaches energy transfers, which up to now were reserved to TOF spectroscopy, while keeping the advantage of the most straightforward data analysis combine with the most wide dynamic range also in the sub-picosecond Fourier time range. This is illustrated by a detailed analysis of both the alpha and beta processes of glass forming polymers close to the glass transition

  15. Going to the limits of NSE

    Energy Technology Data Exchange (ETDEWEB)

    Pappas, C. [BENSC, Hahn Meitner Institut Berlin, Glienickerstrasse 100, 14109 Berlin (Germany)]. E-mail: pappas@hmi.de; Mezei, F. [BENSC, Hahn Meitner Institut Berlin, Glienickerstrasse 100, 14109 Berlin (Germany); Triolo, A. [BENSC, Hahn Meitner Institut Berlin, Glienickerstrasse 100, 14109 Berlin (Germany); IPCF Sezione di Messina, via La Farina 237, 98123 Messina (Italy); Zorn, R. [Institut fuer Festkoerperforschung, Forschungszentrum Juelich, 52425 Juelich (Germany)

    2005-02-15

    Neutron spin echo (NSE) is characterized by a broad variety of new developments. The obvious trend is towards the highest possible energy resolution at the highest possible wavelengths by further developing the generic IN11 design. The planned NSE spectrometer at the SNS will further push the limits of NSE reaching Fourier times as long as 1 {mu}s. On the other hand, simultaneous measurements over higher total solid angles require the real cylindrical symmetry of the magnetic field configuration realized with the spectrometer SPAN at BENSC. The state-of-the-art neutron optical elements lead to considerable gains in neutron flux and allow for extending the wavelength range up to very high wavelengths as well as down to the thermal spectrum. With thermal neutrons NSE reaches energy transfers, which up to now were reserved to TOF spectroscopy, while keeping the advantage of the most straightforward data analysis combine with the most wide dynamic range also in the sub-picosecond Fourier time range. This is illustrated by a detailed analysis of both the alpha and beta processes of glass forming polymers close to the glass transition.

  16. Combining PET/CT with serum tumor markers to improve the evaluation of histological type of suspicious lung cancers.

    Science.gov (United States)

    Jiang, Rifeng; Dong, Ximin; Zhu, Wenzhen; Duan, Qing; Xue, Yunjing; Shen, Yanxia; Zhang, Guopeng

    2017-01-01

    Histological type is important for determining the management of patients with suspicious lung cancers. In this study, PET/CT combined with serum tumor markers were used to evaluate the histological type of lung lesions. Patients with suspicious lung cancers underwent 18F-FDG PET/CT and serum tumor markers detection. SUVmax of the tumor and serum levels of tumor markers were acquired. Differences in SUVmax and serum levels of tumor markers among different histological types of lung cancers and between EGFR mutation statues of adenocarcinoma were compared. The diagnostic efficiencies of SUVmax alone, each serum tumor marker alone, combined tumor markers and the combination of both methods were further assessed and compared. SCC had the highest level of SUVmax, followed by SCLC and adenocarcinoma, and benign lesions had a lowest level. CYFRA21-1 and SCC-Ag were significantly higher in SCC, NSE was significantly higher in SCLC (Ptumor marker or SUVmax alone. When combined, the AUC, sensitivity and specificity increased significantly (Ptumor markers (P>0.05 for all). SUVmax and serum tumor markers show values in evaluating the histological types of suspicious lung cancers. When properly combined, the diagnostic efficiency can increase significantly.

  17. TPA, TPS, and CYFRA 21-1 in Breast Cyst Fluid in Relationship with Possible Breast Cancer Risk

    Czech Academy of Sciences Publication Activity Database

    Šimíčková, M.; Pecen, Ladislav; Vermousek, I.; Černoch, M.; Hudcová, D.

    1997-01-01

    Roč. 18, Suppl.2 (1997), s. 85 ISSN 1010-4283. [Anniversary Meeting of the International Society for Oncodevelopmental Biology and Medicine /25./. 19.09.1997-24.09.1997, Montreaux] Grant - others:IGA MZ(CZ) 3763-3

  18. Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.

    Directory of Open Access Journals (Sweden)

    Jessica J R Hudson

    2011-02-01

    Full Text Available The SMC5-6 protein complex is involved in the cellular response to DNA damage. It is composed of 6-8 polypeptides, of which Nse1, Nse3 and Nse4 form a tight sub-complex. MAGEG1, the mammalian ortholog of Nse3, is the founding member of the MAGE (melanoma-associated antigen protein family and Nse4 is related to the EID (E1A-like inhibitor of differentiation family of transcriptional repressors.Using site-directed mutagenesis, protein-protein interaction analyses and molecular modelling, we have identified a conserved hydrophobic surface on the C-terminal domain of Nse3 that interacts with Nse4 and identified residues in its N-terminal domain that are essential for interaction with Nse1. We show that these interactions are conserved in the human orthologs. Furthermore, interaction of MAGEG1, the mammalian ortholog of Nse3, with NSE4b, one of the mammalian orthologs of Nse4, results in transcriptional co-activation of the nuclear receptor, steroidogenic factor 1 (SF1. In an examination of the evolutionary conservation of the Nse3-Nse4 interactions, we find that several MAGE proteins can interact with at least one of the NSE4/EID proteins.We have found that, despite the evolutionary diversification of the MAGE family, the characteristic hydrophobic surface shared by all MAGE proteins from yeast to humans mediates its binding to NSE4/EID proteins. Our work provides new insights into the interactions, evolution and functions of the enigmatic MAGE proteins.

  19. Clinical usefulness of determination of NSE contents in drainage fluid of patients with chronic subdural hematoma

    International Nuclear Information System (INIS)

    Che Ruchang; Wu Jianyuan; Tao Zhiqiang

    2008-01-01

    Objective: To investigate the relationship between the neuron-specific enolase (NSE) contents of serum and drainage fluid in patients with chronic subdural hematoma (CSDH). Methods: Serum and drainage fluid NSE contents were determined with RIA right after and 24, 48, 72, 96, 120 hours after trephining in 28 patients with CSDH as well as 28 controls (once and serum only). Results: The serum contents of NSE in the patients were significantly higher than those in the controls (P<0.01). The drainage fluid contents of NSE were correlated with the patients concurrent own serum NSE contents (r=0.917) and were higher than the respective serum NSE value (P<0.01). All the NSE contents dropped continuously throughout the observation period. Conclusion: Changes of drainage fluid NSE contents might reflect progress of the degree of nervous tissue injury in patients with chronic subdural hematoma. (authors)

  20. Immunoreactive neuron-specific enolase (NSE) is expressed in testicular carcinoma-in-situ

    DEFF Research Database (Denmark)

    Kang, J L; Rajpert-De Meyts, E; Skakkebaek, N E

    1996-01-01

    Neuron-specific enolase (NSE) is a well-known marker of tumours that have neuroendocrine origin. High levels of NSE have also been described in various types of testicular germ cell neoplasms, particularly in seminomas. To evaluate the presence of NSE in testicular carcinoma-in situ (CIS), a prei...... are evidence against a relationship between NSE and N-myc in testicular germ cell tumours. The high expression of NSE in CIS and overt germ cell tumours may be due to the increased gene dosage effect associated with the overrepresentation of isochromosome 12p....

  1. Elevated Squamous Cell Carcinoma Antigen, Cytokeratin 19 Fragment, and Carcinoembryonic Antigen Levels in Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Jianzhong Chen

    2017-01-01

    Full Text Available Objective. We aimed to explore whether squamous cell carcinoma antigen (SCC, cytokeratin 19 fragment (Cyfra21-1, neuron-specific enolase (NSE, and carcinoembryonic antigen (CEA are elevated in diabetic nephropathy (DN and the association between urinary albumin-to-creatinine ratio (UACR and tumor markers in diabetic patients. Methods. Nondialysis patients with diabetes (n=261 and 90 healthy controls were enrolled. DN was defined as an UACR ≥ 30 mg/g in the absence of a urinary tract infection or other renal abnormalities. Results. Patients with DN had significantly higher serum SCC, Cyfra21-1, and CEA levels than those with normoalbuminuria and healthy controls. The rates of positive SCC, Cyfra21-1, and CEA significantly increased with increasing urinary albumin excretion (all P for trend < 0.001. In contrast, NSE was not affected by DN. SCC, Cyfra21-1, and CEA were significantly and positively correlated with UACR. In logistic regression, after multivariable adjustment, increased UACR was associated with increased odds ratio of elevated tumor marker levels (all P for trend < 0.05. Conclusions. Serum levels of SCC, Cyfra21-1, and CEA are markedly increased with increasing urinary albumin excretion, which affects the specificity for diagnosis for lung cancer. Appropriate interpretation of tumor markers in diabetic patients is mandatory to avoid unnecessary and even hazardous biopsies.

  2. Significance of changes of serum NSE and CEA levels in patients with pneumonia and malignant tumors

    International Nuclear Information System (INIS)

    Liu Hengguo; Luo Nanping; Wang Ruishan; Bai Lu

    2005-01-01

    Objective: To investigate the significance of changes of serum NSE and CEA levels in patients with pneumonia and malignant tumors. Methods: Serum NSE (with RIA) and CEA (with ECLIA) levels in patients with pneumonia or various kinds of malignant tumors (altogether 140 patients) and 32 controls. Results: Serum NSE and CEA levels were significantly higher in patients with lung cancer, gastric cancer, renal cancer, brain tumor and pneumonia than those in the controls (P<0.05,P <0. 05 ,P <0. 01, P<0.01, P<0.01). Positive rate of serum NSE highest in patients with pneumonia, followed successively by renal cancer, brain tumor and lung cancer. NSE levels were positively correlated with CEA levels (r=0.29, P<0.05). Conclusion: As a tumor marker, NSE has important clinical significance in the diagnoses of malignant tumor and pneumonia. (authors)

  3. Detection and significance of S-100 protein and NSE during mild hypothermia cardiopulmonary bypass

    International Nuclear Information System (INIS)

    Liu Xiuqin; Jin Mu; Tan Jiefang; Huang Wenqi; Chen Bingxue; Huang Weiming; Huang Xiongqing

    2001-01-01

    To observe dynamic changes of S-100 protein and NSE during mild hypothermia cardiopulmonary bypass (CPB), the venous blood samples of 25 patients with elective cardiac surgery were obtained simultaneously from the left artery and left jugular bulb before CPB(A), hypothermia period (32-35 degree C) (B) and rewarming to 36 degree C (C) during CPB, 30 minutes (D), 4-6 hours (E) and 24 hours (F) after CPB. Plasma S-100 protein concentration was determined by chemiluminescence immunoassay, and NSE level was determined by radioimmunoassay. The results showed that the levels of S-100 protein and NSE increased significantly during CPB, and NSE peaked at 30 minutes (D) after CPB. It suggested the central nervous system dysfunctions. The S-100 protein and NSE concentrations decreased gradually and retuned to normal nearly (F) after mild hypothermia CPB. It suggested that there were not obvious central nervous system dysfunctions

  4. Wide-angle NSE and TOF the spectrometer SPAN at BENSC

    CERN Document Server

    Pappas, C; Kischnik, R; Mezei, F

    2002-01-01

    The cylindrical symmetry of the magnetic field configuration of SPAN allows for simultaneous neutron spin echo (NSE) measurements over the whole range of scattering angles accessible by a spectrometer. The open construction also allows for time-of-flight (TOF) measurements, which can be performed under the same conditions as NSE, in particular with polarization analysis. TOF and NSE spectra are then directly comparable with each other, without any adjustable parameters, covering a dynamic range of more than four orders of magnitude at a single wavelength. (orig.)

  5. Wide-angle NSE and TOF: the spectrometer SPAN at BENSC

    International Nuclear Information System (INIS)

    Pappas, C.; Triolo, A.; Kischnik, R.; Mezei, F.

    2002-01-01

    The cylindrical symmetry of the magnetic field configuration of SPAN allows for simultaneous neutron spin echo (NSE) measurements over the whole range of scattering angles accessible by a spectrometer. The open construction also allows for time-of-flight (TOF) measurements, which can be performed under the same conditions as NSE, in particular with polarization analysis. TOF and NSE spectra are then directly comparable with each other, without any adjustable parameters, covering a dynamic range of more than four orders of magnitude at a single wavelength. (orig.)

  6. Clinical significance of determination of plasma ET and serum NSE, NPY levels in patients with Alzheimer diseases (AD)

    International Nuclear Information System (INIS)

    Song Hua

    2009-01-01

    Objective: To explore the clinical significance of changes of plasma ET and serum NSE, NPY levels in patients with Alzheimer diseases. Methods: Plasma ET and serum NSE, NPY levels were determined with RIA in 31 patients with Alzheimer diseases and 30 controls. Results: The plasma ET and serum NSE, NPY levels in the patients were significantly higher than those in controls (P<0.01). Plasma ET and serum NSE, NPY levels were mutually positively correlated (r=0.4895, 0.6014, P<0.01). Conclusion: Detection of plasma ET and serum NSE, NPY levels was helpful for the prediction of treatment effieacy in patients with Alzheimer diseases. (authors)

  7. BIOMARKERS S100B AND NSE PREDICT OUTCOME IN HYPOTHERMIA-TREATED ENCEPHALOPATHIC NEWBORNS

    Science.gov (United States)

    Massaro, An N.; Chang, Taeun; Baumgart, Stephen; McCarter, Robert; Nelson, Karin B.; Glass, Penny

    2014-01-01

    Objective To evaluate if serum S100B protein and neuron specific enolase (NSE) measured during therapeutic hypothermia are predictive of neurodevelopmental outcome at 15 months in children with neonatal encephalopathy (NE). Design Prospective longitudinal cohort study Setting A level IV neonatal intensive care unit in a free-standing children’s hospital. Patients Term newborns with moderate to severe NE referred for therapeutic hypothermia during the study period. Interventions Serum NSE and S100B were measured at 0, 12, 24 and 72 hrs of hypothermia. Measurements and Main Reseults Of the 83 infants were enrolled, fifteen (18%) died in the newborn period. Survivors were evaluated by the Bayley Scales of Infant Development (BSID-II) at 15 months of age. Outcomes were assessed in 49/68 (72%) survivors at a mean age of 15.2±2.7 months. Neurodevelopmental outcome was classified by BSID-II Mental (MDI) and Psychomotor (PDI) Developmental Index scores, reflecting cognitive and motor outcomes respectively. Four-level outcome classifications were defined a priori: normal= MDI/PDI within 1SD (>85), mild= MDI/PDI <1SD (70–85), moderate/severe= MDI/PDI <2SD (<70), or died. Elevated serum S100B and NSE levels measured during hypothermia were associated with increasing outcome severity after controlling for baseline and soceioeconomic characteristics in ordinal regression models. Adjusted odds ratios for cognitive outcome were: S100B 2.5 (95% CI 1.3–4.8) and NSE 2.1 (1.2–3.6); for motor outcome: S100B 2.6 (1.2–5.6) and NSE 2.1 (1.2–3.6). Conclusions Serum S100B and NSE levels in babies with NE are associated with neurodevelopmental outcome at 15 months. These putative biomarkers of brain injury may help direct care during therapeutic hypothermia. PMID:24777302

  8. Diagnostic value of combined detection of serum tumor markers for lung cancer

    International Nuclear Information System (INIS)

    Li Yanping; Wang Qun; Zhao Zihong; Zhou Shan

    2013-01-01

    Objective: To investigate the diagnostic value of combined detection of serum tumor markers, including CEA, CA125, neuron-specific enolase (NSE) and cytokeratin fragment antigen 21-1 (CYFRA21-1) for lung cancer patients. Methods: The subjects involved 138 diagnosed lung cancer patients (82 males, 56 females, average age 58.6 years, from October 2010 to March 2012), 96 patients with benign lung diseases (56 males, 40 females, average age 51.3 years) and 45 healthy adults (30 males, 15 females, average age 43.9 years). The pathological types of lung cancer consisted of 66 squamous cell carcinoma (SCC), 52 adenocarcinoma and 20 small cell lung cancer (SCLC). The serum levels of CEA, CA125, NSE and CYFRA21-1 were measured with electrochemiluminescence immunoassay. The diagnostic efficacy for different pathological types was compared among each single tumor marker and combination of tumor markers. One-way analysis of variance q test were used for statistical analysis. Results: The serum levels of CEA, CA125, NSE and CYFRA21-1 in patients with lung cancer were higher than those in patients with benign lung diseases and in healthy subjects (CEA: (19.99±30.99), (10.78±19.77), (3.25±3.42) μg/L; CA125: (79.70±95.98), (44.96±44.97), (20.66±7.13) μg/L; NSE: (35.23±40.22), (15.31±8.42), (13.30±5.65) μg/L; CYFRA21-1: (18.07±43.71), (8.30±8.83), (3.13±1.60) μg/L; F=4.481, 5.436, 4.776, 6.002, all P<0.05). The highest level of CEA, NSE or CYFRA21-1 were found in adenocarcinoma (F=4.932, P<0.05), SCLC (F=5.119, P<0.05) or SCC (F=5.378, P<0.05), respectively. The highest sensitivity tumor markers for SCC, SCLC and adenocarcinoma were CYFRA21-1 (78.8%, 52/66), NSE (75.0%, 15/20) and CEA (57.7%, 30/52), respectively. In combined detection, the highest sensitivity combinations for SCC, SCLC and adenocarcinoma were CEA + CYFRA21-1 + NSE (89.4%, 59/66), CEA + CYFRA21-1 + NSE (80.0%, 16/20) and CEA + CA125 + NSE (78.8%, 41/52), respectively. Conclusions: Combined detection

  9. Clinical Significance of Tumor Marker Detection in Patients 
with Advanced Squamous Cell Carcimoma of the Lung

    Directory of Open Access Journals (Sweden)

    Ping LIANG

    2016-10-01

    Full Text Available Background and objective Due to it's concealment and no obvious symptoms, lung squamous carcimoma often has advanced disease when diagnosed. The aims of this study were to describe the characteristics of the disease, to evaluate the clinical importance of detection of multiple tumor markers in patients with squamous cell carcinoma of the lung. Methods The characteristics of all patients with advanced squamous cell lung cancer treated in Beijing Cancer Hospital of Chinese Academy of Medical Sciences during Jan. 2011 to Dec. 2015 were identified by cases reviewing and data extracting. The characteristics, detection levels and sensitivity of multiple tumor makers among patients were described. Results The 260 patients were treated with mean age of (59.4±9.2 years, 85.8% (n=223 of them were male, 14.2% (n=37 of them were female. 78.1% (n=203 of all were smokers and 3.1% (n=8 of patients had family history of tumor. The positive rate of cytokerantin 19 fragment (CYFRA21-1 was 71.2%, which was the highest among five tumor markers. The five tumor markers median level had no statistical significance between different tumor (T stages and node (N stages (all P>0.05, only the positive rate of SCC had statistical significance between different T stages (P=0.035. The combination measurement of CYFRA21-1+carcinogen-embryonic antigen (CEA, CYFRA21-1+CEA+cancer antigen (CA125, CA125+CYFRA21-1+CEA+neuron specific enolase (NSE, and CA125+CYFRA21-1+NSE+CEA+squamous cell carcinoma antigen (SCC were better and had higher clinical values, the positive rates were 82.7%, 84.6%, 85.0% and 86.2%, respectively. Conclusion The positive rate of CYFRA21-1 was the highest and the sensitivity of single test of five tumor markers was low, the combination of multiple tumor markers increased the sensitivity of diagnosis of SQCLC, the combination of CA125, CYFRA21-1 and CEA was the best choice.

  10. Predictive and prognostic value of preoperative serum tumor markers is EGFR mutation-specific in resectable non-small-cell lung cancer

    Science.gov (United States)

    Jiang, Richeng; Wang, Xinyue; Li, Kai

    2016-01-01

    Background The predictive and prognostic value of carcinoembryonic antigen (CEA), cytokeratin-19 fragments (Cyfra21-1), squamous cell carcinoma antigen (SCCA) and neuron-specific enolase (NSE) has been investigated in non-small-cell lung cancer (NSCLC) patients. However, few studies have directly focused on the association between these markers and epidermal growth factor receptor (EGFR) mutation status or mutation subtypes. Patients and methods We retrospectively analyzed 1016 patients with stage I-IIIA NSCLC who underwent complete resection between 2008 and 2012. Correlations between serum tumor marker levels and EGFR mutations and survival parameters were analyzed and prognostic factors were identified. Results Cyfra21-1 levels (P = 0.032 for disease-free survival [DFS]; P CEA levels (P CEA (P = 0.005) and clinical stage were predictive factors of DFS, while elevated CEA (P = 0.005) and Cyfra21-1 (P = 0.027) were independent prognostic factors. Conclusion Cyfra21-1 and CEA exhibit different predictive and prognostic values between EGFR-mutated and wild-type adenocarcinomas, as well as between EGFR mutation subtypes. The prognostic impact of preoperative serum tumor markers should be evaluated together with EGFR mutation status. PMID:27072585

  11. Study on effect of Nimodepine on the changes of serum NSE levels in patients with acute cerebral hemorrhage

    International Nuclear Information System (INIS)

    Zhang Chunying; Li Zuoxiao; Gan Xilun; Li Xiaohong; Tan Hua

    2006-01-01

    Objective: To investigate the changes of serum NSE levels in patients with acute cerebral hemorrhage and the effect of nimodepine treatment. Methods: Serum neuron specific enolase (NSE) levels were measured with CLIA in 60 patients with cerebral hemorrhage both before and after treatment as well as in 30 controls. Half of the patients (n=30) were treated with nimodepine and the their half were not. Results: In all the 60 patients, serum NSE levels were significantly higher than those in Controls (P<0.01). After treatment, the NSE levels dropped markedly in all the patients. However, the decrease in the patient group treated with nimodepine was significantly higher than that in the patient group treated without nimodepine (P<0.01). Conclusion: Nimodepine treatment is efficient for reducing the serum NSE levels, which may be related to the residual hematoma size. (authors)

  12. Clinical significance of changes of serum NSE, TNF-α and IL-6 levels in patients with hypoxic ischemic encephalopathy

    International Nuclear Information System (INIS)

    Zhang Yuhong; Zhang Yujuan; Zhou Xiujuan; Shan Huali

    2010-01-01

    Objective: To study the clinical significance of changes of serum NSE, TNF-α and IL-6 levels in neonates with hypoxic ischemic encephalopathy. Methods: Serum NSE (with ELISA) and TNF-α, IL-6 (with RIA) levels were measured in 30 neonates with hypoxic ischemic encephalopathy and 30 controls. Results: Serum NSE, TNF-α and IL-6 levels were significantly higher in neonates with hypoxic-ischemic encephalopathy than those in controls (P<0.01). Serum NSE levels were positively correlated with those of TNF-α, IL-6 (r=0.5812, 0.6014, P<0.01). Conclusion: Serum NSE, TNF-α and IL-6 levels were closely related to the diseases process of hypoxic-ischemic encephalopathy. (authors)

  13. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons.

    Directory of Open Access Journals (Sweden)

    Mandy Ka Man Kam

    Full Text Available To establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer, which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult.

  14. NSE, CEA and SCC - a useful combination of tumor markers in lung cancer

    International Nuclear Information System (INIS)

    Fischbach, W.; Jany, B.

    1988-01-01

    The usefulness of neuronspecific enolase (NSE), CEA, and of the tumor associated antigen SSC was investigated in 61 patients with histologically proven lung cancer (small cell lung cancer n=25, adenocarcinoma n=14, squamous cell carcinoma n=18 and large cell carcinoma n=4). The sensitivity of NSE was 93.3% in small cell lung cancer (SCLC), whereas in adeno- and squamous cell carcinoma only 8 or 13%, resp., elevated serum NSE were found. CEA was the most sensitive marker for adenocarcinoma (58.3%). Contrary to NSE, however, CEA does not allow any conclusions concerning differential diagnosis as pathological serum concentrations were also observed in 46.6% both in small cell lung cancer and in squamous cell carcinoma. SCC demonstrated a sensitivity of 53% in squamous cell carcinoma. Elevated serum levels were also found in adenocarcinoma (41.6%), but never in small lung cancer. For all three markers tested, high serum concentrations were predominantly present in patients with advanced disease state. (orig.) [de

  15. TOPLAR: Time of Flight with Larmor Precessions - or - How to extend the dynamic range of NSE spectrometers

    International Nuclear Information System (INIS)

    Van Well, A.A.; Bleuel, M.; Pappas, C.

    2011-01-01

    Neutron Spin Echo (NSE) spectrometers typically cover a dynamic range of three orders of magnitude at a given wavelength. At long Fourier times the limits are given by the homogeneity of precession fields. At short Fourier times, the quasi-elastic approximation and the NSE formalism mark a methodological limit. We propose to overcome this limitation and by combining Time Of Flight with Larmor precession to extend the capabilities of Neutron Spin Echo spectrometers towards short Fourier times. TOFLAR should be easily implemented on NSE spectrometers equipped with a chopper system such as IN15 or the planned WASP. (authors)

  16. The Combination of the Tumor Markers Suggests the Histological Diagnosis of Lung Cancer

    Directory of Open Access Journals (Sweden)

    Linjie Liu

    2017-01-01

    Full Text Available Tumor markers are beneficial for the diagnosis and therapy monitoring of lung cancer. However, the value of tumor markers in lung cancer histological diagnosis is unknown. In this study, we analyzed the serum levels of six tumor markers (CEA, CYFRA21-1, SCC, NSE, ProGRP, and CA125 in 2097 suspected patients with lung cancer and determined whether the combination of the tumor markers was useful for histological diagnosis of lung cancer. We found that CYFRA21-1 was the most sensitive marker in NSCLC. ProGRP showed a better clinical performance than that of NSE in discriminating between SCLC and NSCLC. The serum level of CYFRA21-1 or SCC was significantly higher in squamous carcinoma (p<0.05, and the levels of ProGRP and NSE were significantly higher in SCLC (p<0.05. According to the criteria established, SCLC and NSCLC were discriminated with sensitivity of 87.12 and 62.63% and specificity of 64.61 and 99.5%, respectively. The sensitivity and specificity in the differentiation of adenocarcinoma and squamous carcinoma were 68.1 and 81.63% and 70.73 and 65.93%, with NPV of 46.03 and 68.97% and PPV of 85.82 and 79.47%, respectively. Our results suggested the combination of six tumor markers could discriminate the histological types of lung cancer.

  17. Decomposition of the Mean Squared Error and NSE Performance Criteria: Implications for Improving Hydrological Modelling

    Science.gov (United States)

    Gupta, Hoshin V.; Kling, Harald; Yilmaz, Koray K.; Martinez-Baquero, Guillermo F.

    2009-01-01

    The mean squared error (MSE) and the related normalization, the Nash-Sutcliffe efficiency (NSE), are the two criteria most widely used for calibration and evaluation of hydrological models with observed data. Here, we present a diagnostically interesting decomposition of NSE (and hence MSE), which facilitates analysis of the relative importance of its different components in the context of hydrological modelling, and show how model calibration problems can arise due to interactions among these components. The analysis is illustrated by calibrating a simple conceptual precipitation-runoff model to daily data for a number of Austrian basins having a broad range of hydro-meteorological characteristics. Evaluation of the results clearly demonstrates the problems that can be associated with any calibration based on the NSE (or MSE) criterion. While we propose and test an alternative criterion that can help to reduce model calibration problems, the primary purpose of this study is not to present an improved measure of model performance. Instead, we seek to show that there are systematic problems inherent with any optimization based on formulations related to the MSE. The analysis and results have implications to the manner in which we calibrate and evaluate environmental models; we discuss these and suggest possible ways forward that may move us towards an improved and diagnostically meaningful approach to model performance evaluation and identification.

  18. Neuronal markers are expressed in human gliomas and NSE knockdown sensitizes glioblastoma cells to radiotherapy and temozolomide

    International Nuclear Information System (INIS)

    Yan, Tao; Skaftnesmo, Kai Ove; Leiss, Lina; Sleire, Linda; Wang, Jian; Li, Xingang; Enger, Per Øyvind

    2011-01-01

    Expression of neuronal elements has been identified in various glial tumors, and glioblastomas (GBMs) with neuronal differentiation patterns have reportedly been associated with longer survival. However, the neuronal class III β-tubulin has been linked to increasing malignancy in astrocytomas. Thus, the significance of neuronal markers in gliomas is not established. The expressions of class III β-tubulin, neurofilament protein (NFP), microtubule-associated protein 2 (MAP2) and neuron-specific enolase (NSE) were investigated in five GBM cell lines and two GBM biopsies with immunocytochemistry and Western blot. Moreover, the expression levels were quantified by real-time qPCR under different culture conditions. Following NSE siRNA treatment we used Electric cell-substrate impedance sensing (ECIS) to monitor cell growth and migration and MTS assays to study viability after irradiation and temozolomide treatment. Finally, we quantitated NSE expression in a series of human glioma biopsies with immunohistochemistry using a morphometry software, and collected survival data for the corresponding patients. The biopsies were then grouped according to expression in two halves which were compared by survival analysis. Immunocytochemistry and Western blotting showed that all markers except NFP were expressed both in GBM cell lines and biopsies. Notably, qPCR demonstrated that NSE was upregulated in cellular stress conditions, such as serum-starvation and hypoxia, while we found no uniform pattern for the other markers. NSE knockdown reduced the migration of glioma cells, sensitized them to hypoxia, radio- and chemotherapy. Furthermore, we found that GBM patients in the group with the highest NSE expression lived significantly shorter than patients in the low-expression group. Neuronal markers are aberrantly expressed in human GBMs, and NSE is consistently upregulated in different cellular stress conditions. Knockdown of NSE reduces the migration of GBM cells and sensitizes

  19. Chronic exercise ameliorates the neuroinflammation in mice carrying NSE/htau23

    International Nuclear Information System (INIS)

    Leem, Yea-Hyun; Lee, Young-Ik; Son, Hee-Jeong; Lee, Sang-Ho

    2011-01-01

    Research highlights: → The progress of neurodegeration are directly linked to the neuroinflammatory response. → We investigate whether exercise improves the neuroinflammation using T g -NSE/htau23 mice. → This provides insights that exercise may beneficial effects on the neuroinflammatory disorders. -- Abstract: The objective of the present study was to investigate whether chronic endurance exercise attenuates the neuroinflammation in the brain of mice with NSE/htau23. In this study, the tau-transgenic (Tg) mouse, Tg-NSE/htau23, which over expresses human Tau23 in its brain, was subjected to chronic exercise for 3 months, from 16 months of age. The brains of Tg mice exhibited increased immunoreactivity and active morphological changes in GFAP (astrocyte marker) and MAC-1 (microglia marker) expression in an age-dependent manner. To identify the effects of chronic exercise on gliosis, the exercised Tg mice groups were treadmill run at a speed of 12 m/min (intermediate exercise group) or 19 m/min (high exercise group) for 1 h/day and 5 days/week during the 3 month period. The neuroinflammatory response characterized by activated astroglia and microglia was significantly repressed in the exercised Tg mice in an exercise intensity-dependent manner. In parallel, chronic exercise in Tg mice reduced the increased expression of TNF-α, IL-6, IL-1β, COX-2, and iNOS. Consistently with these changes, the levels of phospho-p38 and phospho-ERK were markedly downregulated in the brain of Tg mice after exercise. In addition, nuclear NF-κB activity was profoundly reduced after chronic exercise in an exercise intensity-dependent manner. These findings suggest that chronic endurance exercise may alleviate neuroinflammation in the Tau pathology of Alzheimer's disease.

  20. Dynamical scaling and critical scattering in pure and disordered ferromagnets probed by NSE

    Energy Technology Data Exchange (ETDEWEB)

    Alba, M. [LLB, CEA-CNRS UMR12, CEA-Saclay, 91191 Gif/Yvette Cedex (France)]. E-mail: michel.alba@cea.fr; Pouget, S. [DRFMC/SPSMS, CEN-Grenoble, 17 rue des Martyrs, 38054 Grenoble (France); Fouquet, P. [ILL, 6 rue Jules Horowitz, 38042 Grenoble (France); Farago, B. [ILL, 6 rue Jules Horowitz, 38042 Grenoble (France); Pappas, C. [Hahn-Meitner Institut, Glienickerstr. 100, 14109 Berlin (Germany)

    2007-07-15

    We have studied the 3D Heisenberg ferromagnetic model system CdCr{sub 2} {sub x} In{sub 2-2} {sub x} S{sub 4} in the ferromagnetic and reentrant phases as a function of temperature and momentum transfer using neutron spin echo (NSE) spectroscopy. The results from the pure sample CdCr{sub 2}S{sub 4} are in excellent agreement with the predictions of the renormalization group theory. In the presence of disorder, we see the evolution from a simple critical ferromagnetic scattering with single fast relaxation times to a more complex slow dynamics characteristic of spin glasses.

  1. Brain injury markers (S100B and NSE) in chronic cocaine dependents

    OpenAIRE

    Kessler, Felix Henrique Paim; Woody, George; Portela, Luís Valmor Cruz; Tort, Adriano Bretanha Lopes; De Boni, Raquel; Peuker, Ana Carolina Wolf Baldino; Genro, Vanessa; Diemen, Lísia von; Souza, Diogo Onofre Gomes de; Pechansky, Flavio

    2007-01-01

    OBJECTIVE: Studies have shown signs of brain damage caused by different mechanisms in cocaine users. The serum neuron specific enolase and S100B protein are considered specific biochemical markers of neuronal and glial cell injury. This study aimed at comparing blood levels of S100B and NSE in chronic cocaine users and in volunteers who did not use cocaine or other illicit drugs. METHOD: Twenty subjects dependent on cocaine but not on alcohol or marijuana, and 20 non-substance using controls ...

  2. Diagnostic utility of neuron specific enolase (NSE) in serum and pleural fluids from patients with lung cancer and tuberculosis

    International Nuclear Information System (INIS)

    Alam, J.M.; Baig, J.A.; Asghar, S.S.; Mahmood, S.R.; Ansari, M.A.; Jamil, S.

    2010-01-01

    Several past and recent investigations have focused on the determination of tumor markers in pleural fluids to assess their Usefulness as less invasive replacement method of diagnosis. In this regard, few studies have dealt with the determination of the tumor marker, neuron specific enolase (NSE), in pleural fluids of patients suffering from both benign and malignant diseases such as non small cell lung carcinoma( NSCLC), small cell lung carcinoma( SCLC) and tuberculosis. Therefore, the present study was undertaken to establish the diagnostic utility of NSE in malignant condition by assessing levels in serum and pleural fluids of patients with lung cancer and by comparing it with a benign pulmonary disease of tuberculosis. Pleural fluids were obtained from 22 patients with carcinomatous pleurisy due to SCLC, 11 patients with carcinomatous pleurisy due to non-small cell lung cancer, and 30 patients with tuberculosis pleurisy for comparison purpose. Determination of NSE levels was performed by ECL technology according to the manufacturer's instructions. NSE levels of pleural fluids from SCLC patients were significantly elevated( P<0.0001) when compared with pleural fluids from NSCLC and tuberculosis patients. Moreover, pleural fluids of all 30 tuberculosis patients and 11 NSCLC patients showed moderate significance ( P< O.05 and P < 0.01, respectively) when compared with each other. In addition, cumulative results of NSE levels from SCLC and NSCLC combined also showed high significance (P<0.001) as compared to pleural fluids of tuberculosis patients and moderate significance (P<0.01) when compared with serum levels of both malignant and benign groups. It is concluded that determination of NSE levels in pleural fluids of lung cancer patients noted to be an effective diagnostic tool to differentiate carcinomatous pleurisy due to SCLC from those occurring due to NSCLC and tuberculosis. Further studies with larger group of patients are under progress to further establish

  3. Clinical significance of determination of plasma Leptin and serum Hcy, S100B and NSE levels in patients with alzheimer disease

    International Nuclear Information System (INIS)

    Dou Huanzhi; Lu Meng

    2011-01-01

    To explore the clinical significance of changes of plasma leptin and serun Hcy, S100B and NSE levels in patients with Alzheimer Disease (AD). The plasma leptin and serum NSE levels in 32 AD patients and 30 controls were determined by using RIA, and the serum Hcy and S100B levels were measured by using CLIA. The results showed that the plasma leptin and serun Hcy, S100B and NSE levels in AD patients were significantly higher than these in controls (P<0.01). The plasma leptin levels in AD patients was mutually positively correlated with serum Hcy, S100B and NSE levels (r=0.5982, 0.4762, 0.6014, P<0.01). The detection of plasma leptin and serum Hcy, S100B and NSE levels may be helpful for the prediction of treatment efficiency in patients with Alzheimer disease. (authors)

  4. Diagnostic value of multiple tumor markers for patients with esophageal carcinoma.

    Science.gov (United States)

    Zhang, Jun; Zhu, Zhenli; Liu, Yan; Jin, Xueyuan; Xu, Zhiwei; Yu, Qiuyan; Li, Ke

    2015-01-01

    Various studies assessing the diagnostic value of serum tumor markers in patients with esophageal cancer remain controversial. This study aims to comprehensively and quantitatively summarize the potential diagnostic value of 5 serum tumour markers in esophageal cancer. We systematically searched PubMed, Embase, Chinese National Knowledge Infrastructure (CNKI) and Chinese Biomedical Database (CBM), through February 28, 2013, without language restriction. Studies were assessed for quality using QUADAS (quality assessment of studies of diagnostic accuracy). The positive likelihood ratio (PLR) and negative likelihood ratio (NLR) were pooled separately and compared with overall accuracy measures using diagnostic odds ratios (DORs) and symmetric summary receiver operating characteristic (SROC) curves. Of 4391 studies initially identified, 44 eligible studies including five tumor markers met the inclusion criteria for the meta-analysis, while meta-analysis could not be conducted for 12 other tumor markers. Approximately 79.55% (35/44) of the included studies were of relatively high quality (QUADAS score≥7). The summary estimates of the positive likelihood ratio (PLR), negative likelihood ratio (NLR) and diagnostic odds ratio (DOR) for diagnosing EC were as follows: CEA, 5.94/0.76/9.26; Cyfra21-1, 12.110.59/22.27; p53 antibody, 6.71/0.75/9.60; SCC-Ag, 7.66/0.68/12.41; and VEGF-C, 0.74/0.37/8.12. The estimated summary receiver operating characteristic curves showed that the performance of all five tumor markers was reasonable. The current evidence suggests that CEA, Cyfra21-1, p53, SCC-Ag and VEGF-C have a potential diagnostic value for esophageal carcinoma.

  5. Effect of early rehabilitation training on the serum NGF, NSE, BDNF, and motor function in patients with acute cerebral infarction

    Institute of Scientific and Technical Information of China (English)

    Zhen-Ping Cui

    2017-01-01

    Objective:To explore the effect of early rehabilitation training on the serum NGF, NSE, BDNF, and motor function in patients with acute cerebral infarction (ACI).Methods: A total of 150 patients with ACI who were admitted in our hospital from October, 2015 to October, 2016 were included in the study and randomized into the observation group and the control group. The patients in the two groups were given anti-coagulation, anti-platelet aggregation, cerebral circulation improving, and brain cell activators. The patients in the control group were given routine neurological nursing, while the patients in the observation group were given early comprehensive rehabilitation training on the basis of stable vital signs and no disease progression within 48 h. The morning fasting peripheral venous blood before treatment, 2 and 4 weeks after treatment in the two groups was collected. The serum NGF, NSE, BDNF, IL-6, hs-CRP, and TNF-α were detected. The activities of daily living and motor function before treatment, 2 and 4 weeks after treatment in the two groups were evaluated.Results:IL-6, hs-CRP, and TNF-α levels 2 and 4 weeks after treatment in the observation group were significantly lower than those in the control group (P<0.05). NGF and BDNF levels 2 and 4 weeks after treatment in the observation group were significantly higher than those in the control group (P<0.05), while NSE level was significantly lower than that in the control group (P<0.05). MBI and FMA scores 2 and 4 weeks after treatment in the observation group were significantly higher than those in the control group (P<0.05).Conclusions: The early rehabilitation training can effectively reduce the inflammatory reaction of nervous system in patients with ACI, regulate NGF, BDNF, and NSE levels, and play the cerebral function remodeling in order to promote the neural function recovery, and improve the motor function.

  6. Diagnostic value of combined determination of serum tumor markers (NSE, CA-242, TPA, CEA) levels in patients with lung cancer

    International Nuclear Information System (INIS)

    Liu Juzhen; Cai Tietie; Qin Shana

    2007-01-01

    Objective: To investigate the diagnostic value of combined determination of serum NSE, CA242, tissue polypeptide antigen (TPA) and CEA levels in patients with primary lung cancer. Methods: Serum NSE, CA242, TPA and CEA levels were determined with ELISA in (1) 102 patients with various types of primary lung carcinoma (adenocarcinoma 38, squamous cell carcinoma 32, small cell lung carcinoma 32) (2) 33 patients with open lung T. B. and (3) 30 controls. Results: (1) In patients with lung cancer, serum levels of all the four markers were increased and significantly higher than their respective values in patients with open lung T.B. and controls. (2) Positive rate of combined any two markers were 75% for adenocarcinoma, 50% for squamous cell carcinoma and 65% for small cell lung carcinoma, while false positive rate was only 9% for T.B patients and none for the controls. (3) The most appropriate single marker for each specific type of lung cancer was: NSE for SCLC (sensitivity 72%, specificity 97%, CA242 for adenocarcinoma sensitivity 62%, specificity 90%). Conclusion: Combined determination of these tumor markers would improve the sensitivity and specificity for diagnosis of primary lung carcinoma. (authors)

  7. CEA, SCC and NSE levels in exhaled breath condensate--possible markers for early detection of lung cancer.

    Science.gov (United States)

    Zou, Yingchang; Wang, Lin; Zhao, Cong; Hu, Yanjie; Xu, Shan; Ying, Kejing; Wang, Ping; Chen, Xing

    2013-12-01

    Lung cancer (LC) is the leading cause of cancer-related death. The sensitive and non-invasive diagnostic tools in the early stage are still poor. We present a pilot study on the early diagnosis of LC by detecting markers in exhaled breath condensate (EBC). EBC samples were collected from 105 patients with LC and 56 healthy controls. We applied chemiluminescence immunoassay to detect CEA (carcinoembryonic antigen), SCC (squamous cell carcinoma) antigen and NSE (neuron specific enolase) in EBC and serum. Concentrations of markers were compared between independent groups and subgroups. A significantly higher concentration level of each marker was found in patients with LC than healthy controls. The areas under curve of receiver operating characteristic (ROC) curves were 0.800, 0.771, 0.659, 0.679, 0.636 and 0.626 for EBC-CEA, serum-CEA, EBC-SCC, serum-SCC, EBC-NSE and serum-NSE, respectively. Markers in EBC had a higher positive rate (PR) and were more specific to histologic types than markers in serum. In addition, multivariate analysis was performed to evaluate the association of presenting markers with the stages of non-small cell lung cancer (NSCLC). EBC-CEA showed the best predictive characteristic (p tumor markers in EBC may have a better diagnostic performance for LC than those in serum. With further investigation on the combination of markers in EBC, detection of EBC could probably be a novel and non-invasive method to detect NSCLC earlier.

  8. Clinical significance of measurement of changes of serum NSE and plasma NPY levels after treatment in pediatric patients with viral encephalitis

    International Nuclear Information System (INIS)

    Jin Bo; Zheng Guo

    2007-01-01

    Objective: To explore the significance ef changes of serum NSE and plasma NPY levels after treatment in pediatric patients with viral encephalitis. Methods: Serum NSE and plasma NPY levels were measured with RIA in 32 pediatric patients with viral encephalitis both before and after treatment as well as in 30 controls. Results: Before treatment, in the patients, the serum NSE and plasma NPY levels were significantly higher than those in controls (P<0.01). After 1 month's treatment the levels dropped markedly but still remained significantly higher than those in controls (P<0.05). Conclusion: Serum NSE and plasma NPY levels changes were closely related to the progress of viral encephalitis. (authors)

  9. Clinical significance of determination of changes of serum NSE, SIL-2R and TNF levels in patients with lung cancer undergoing chemotherapy

    International Nuclear Information System (INIS)

    Tan Zongxian

    2005-01-01

    Objective: To detect the changes of serum NSE, SIL-2R and TNF levels in the 33 patients with lung cancer undergoing chemotherapy. Methods: Serum NSE, SIL-2R and TNF levels were determined with RIA and SIL-2R levels with ELISA in 33 lung cancer patients both before and after chemotherapy (n=28) as well as in 30 controls. Results: Before chemotherapy, serum NSE, SIL-2R and TNF levels in the patients were significantly higher than those in the controls (P<0.01). After chemotherapy, in 20 cases without recurrence at 6 months, the levels were much lower but still significantly higher than those in controls (P < 0.05 ). However, in the 8 patients with recurrence, the levels increased again to approaching those before chemotherapy. Conclusion: Serum levels of NSE, SIL-2R and TNF might be useful for diagnosis and predicting therapeutic effects after chemotherapy in patients with lung cancer. (authors)

  10. Clinical significance of determination of changes of serum NSE, IGF-II and TNF-α levels after chemotherapy in patients with lung cancer

    International Nuclear Information System (INIS)

    Ji Yajun; Yang Chengxi; Bian Baoxiang; Song Ziyan

    2008-01-01

    Objective: To detect the changes of serum NSE, IGF-II and TNF-α levels after chemotherapy in patients with lung cancer. Methods: Serum NSE, IGF-II and TNF-α levels were determined with RIA in 38 patients with lung cancer both be- fore and after chemotherapy as well as in 35 controls. Results: Before chemotherapy, serum NSE, IGF-II and TNF-α levels in the patients were significantly higher than those in the controls (P<0.01), After chemotherapy, in 25 cases without recurrence at 6 months, the levels were remained dropped markedly and approached those in controls. However in the 5 patients with recurrence, the levels increased again, approaching those before chemotherapy. Conclusion: Serum levels of NSE, IGF-II and TNF-α might be useful for diagnosis and predicting therapeutic effects after chemotherapy in patients with lung cancer. (authors)

  11. Clinical significance of determination of changes of serum CEA, NSE, CA19-9 and VEGF levels in patients with lung cancer

    International Nuclear Information System (INIS)

    Gu Yan; Wang Yuyi

    2009-01-01

    Objective: To explore the clinical significance of changes of serum CEA, NSE, CA19-9 and VEGF levels in patients with lung cancer. Methods: Serum CEA, NES, CA19-9 (with RIA) and VEGF (with ELISA) levels were detected in 31 patients with lung cancer and 35 controls. Results: The levels of serum CEA, NSE, CA19-9 and VEGF were significantly higher in the patients than those in controls (P<0.01). Serum CEA, NSE, CA19-9 levels were positively correlated with the VEGF levels (r=0.6218, 0.6101, 0.6317, P<0.01). Conclusion: Serum CEA, NSE, CA19-9 and VEGF levels were closely related to the diseases process of lung cancer and were of prognostic values. (authors)

  12. Change and significance of serum inflammatory factors, NSE, S100 protein and stress hormone levels in patients with craniocerebral injury

    Directory of Open Access Journals (Sweden)

    Rui-Feng Liu

    2017-09-01

    Full Text Available Objective: To investigate the change and significance of serum inflammatory factors, neuron specific enolase (NSE, S100 protein and stress hormone levels in patients with brain diseases. Methods: A total of 115 patients with craniocerebral injury were selected as the observation group, according to the Glasgow Coma Scale (GCS, they were divided into light-sized group (n=38, middle-sized group (n=40 and severe-sized group (n=37, at the same time the other 120 healthy subjects were selected as the control group. The levels of serum inflammatory cytokines [tumor necrosis factor alpha (TNF-α and procalcitonin (PCT], neuron specific enolase (NSE, S100 protein and the stress hormone cortisol [(COR, adrenocorticotropic hormone (ACTH, β-endorphin (β-EP] of both groups were compared. Results: The levels of TNF-α, PCT, NSE, S100, COR, ACTH and β-EP in the observation group were (145.73±19.24 ng/L, (2.41±0.64 ng/mL, (38.11±12.28 ng/mL, (0.87±0.32 μg/L, (818.87±121.14 nmol/L, (107.38±13.94 ng/L, (126.74±39.04 ng/mL, which were significantly higher than control group, the difference was statistically significant; Comparison of indexes among the observation group, NF-α, PCT, NSE, S100, COR, ACTH and β-EP levels in the middle-sized group and severe-sized group were significantly higher than those in the light-sized group, and the levels in the severe-sized group were significantly higher than those of the middle-sized group, the difference was statistically significant. Conclusion: The levels of Serum inflammatory factors, NSE, S100 protein and stress hormone were significantly increased in patients with craniocerebral injury, the level was related to the degree of traumatic brain injury, which could be used as an important indicator to assess the severity of the disease.

  13. Clinical value of combined determination of serum and hydrothorax fluid levels of CEA, CA125, NSE in the diagnosis of lung cancer

    International Nuclear Information System (INIS)

    Su Wentang; Shu Lingling; Yang Huaxi

    2007-01-01

    Objective: To study the clinical value of combined determination of CEA, CA125, NSE levels both in serum and hydrothorax fluid in the diagnosis of lung cancer. Methods: Serum and hydrothorax fluid levels of CEA, CA125, NSE were determined with RIA in 88 patients with lung cancers, 100 patients with inflammatory hydrothorax, and 50 controls. Results: The levels of serum and hydrothorax fluid CEA, CA125, NSE in lung cancer patients were significantly higher than those in patients with inflammatory hydrothorax and controls (P <0.05). In lung cancer group, the positive rate of combined detection of serum CEA, CA125, NSE was 70.5%, the positive rate of combined detection of hydrothorax fluid CEA, CA125, NSE was 79.5% and the positive rate of combined detection of serum and hydrothorax fluid three kinds of tumor markers was 87. 5%. Conclusion: Combined detection of serum and hydrothuax fluid levels of CEA, CA125, NSE is to be advocated because of higher sensitivity for diagnosis of lung cancer. (authors)

  14. Sexual behavior among Brazilian adolescents, National Adolescent School-based Health Survey (PeNSE 2012).

    Science.gov (United States)

    Oliveira-Campos, Maryane; Nunes, Marília Lavocart; Madeira, Fátima de Carvalho; Santos, Maria Goreth; Bregmann, Silvia Reise; Malta, Deborah Carvalho; Giatti, Luana; Barreto, Sandhi Maria

    2014-01-01

    This study describes the sexual behavior among students who participated in the National Adolescent School-based Health Survey (PeNSE) 2012 and investigates whether social inequalities, the use of psychoactive substances and the dissemination of information on sexual and reproductive health in school are associated with differences in behavior. The response variable was the sexual behavior described in three categories (never had sexual intercourse, had protected sexual intercourse, had unprotected sexual intercourse). The explanatory variables were grouped into socio- demographic characteristics, substance use and information on sexual and reproductive health in school. Variables associated with the conduct and unprotected sex were identified through multinomial logistic regression, using "never had sexual intercourse" as a reference. Over nearly a quarter of the adolescents have had sexual intercourse in life, being more frequent among boys. About 25% did not use a condom in the last intercourse. Low maternal education and work increased the chance of risky sexual behavior. Any chance of protected and unprotected sex increased with the number of psychoactive substances used. Among those who don't receive guidance on the prevention of pregnancy in school, the chance to have sexual intercourse increased, with the largest magnitude for unprotected sex (OR = 1.41 and OR = 1.87 ). The information on preventing pregnancy and STD/AIDS need to be disseminated before the 9th grade. Social inequalities negatively affect risky sexual behavior. Substance use is strongly associated with unprotected sex. Information on the prevention of pregnancy and STD/AIDS need to be disseminated early.

  15. Brain injury markers (S100B and NSE) in chronic cocaine dependents Marcadores de lesão cerebral (S100B e NSE) em dependentes crônicos de cocaína

    OpenAIRE

    Felix Henrique Paim Kessler; George Woody; Luís Valmor Cruz Portela; Adriano Bretanha Lopes Tort; Raquel De Boni; Ana Carolina Wolf Baldino Peuker; Vanessa Genro; Lísia von Diemen; Diogo Onofre Gomes de Souza; Flavio Pechansky

    2007-01-01

    OBJECTIVE: Studies have shown signs of brain damage caused by different mechanisms in cocaine users. The serum neuron specific enolase and S100B protein are considered specific biochemical markers of neuronal and glial cell injury. This study aimed at comparing blood levels of S100B and NSE in chronic cocaine users and in volunteers who did not use cocaine or other illicit drugs. METHOD: Twenty subjects dependent on cocaine but not on alcohol or marijuana, and 20 non-substance using controls ...

  16. Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays

    Science.gov (United States)

    Geißler, D.; Hill, D.; Löhmannsröben, H.-G.; Thomas, E.; Lavigne, A.; Darbouret, B.; Bois, E.; Charbonnière, L. J.; Ziessel, R. F.; Hildebrandt, N.

    2010-02-01

    An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.

  17. Clinical significance of changes of serum NSE, cortisol, ADM, calcium, magnesium levels and platalet count in neonates with hypoxic-ischemic encephalopathy (HIE)

    International Nuclear Information System (INIS)

    Lv Weihua; Huang Weiliang

    2009-01-01

    Objective: To study the clinical significance of changes of serum NSE, cortisol, ADM, Ca ++ , Mg ++ levels and platalet count in neonates with HIE. Methods: Serum NSE, ADM(with RIA), cortisol(with CLIA), Ca ++ and Mg ++ (with biochemistry) levels and platalet count were determined in 52 neonates with HIE(mild, n=26, moderate, n=16, advanced, n=10) and 30 controls. Results: In the neonates with mild HIE, the serum NSE and ADM levels were not significantly different from those in controls (P>0.05) but cortisol levels were significantly higher (P ++ and Mg ++ levels as well as decrease of platelet count, might reflect the severity of the disease process of HIE in neonates and might be of prognostic importance. (authors)

  18. Correlation of serum GFAP, S100B and NSE contents with posttraumatic oxidative stress response and insulin resistance in patients with traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Bing-Feng Tian

    2018-07-01

    Full Text Available Objective: To study the correlation of serum GFAP, S100B and NSE contents with posttraumatic oxidative stress response and insulin resistance in patients with traumatic brain injury. Methods: A total of 110 patients with traumatic brain injury who were treated in our hospital between January 2015 and December 2016 were collected as the observation group, and 60 healthy subjects who received physical examination in our hospital during the same period were collected as normal control group. Serum GFAP, S100B and NSE levels as well as oxidative stress index and insulin resistance index levels of two groups of subjects were detected, and Pearson test was used to further evaluate the correlation of serum GFAP, S100B and NSE contents with oxidative stress response and insulin resistance in patients with traumatic brain injury. Results: Serum GFAP, S100B and NSE contents of observation group were significantly higher than those of normal control group; serum oxidative stress indexes MDA, MPO and LPO contents were higher than those of normal control group while SOD and TAC contents were lower than those of normal control group; serum insulin resistance indexes GLU, INS and HOMA-IR levels were higher than those of control group. Pearson test showed that serum GFAP, S100B and NSE contents in patients with traumatic brain injury were directly correlated with post-traumatic oxidative stress and insulin resistance. Conclusion: The serum GFAP, S100B and NSE contents increase in patients with traumatic brain injury, and the increase is directly correlated with the oxidative stress and insulin resistance.

  19. Softening of phospholipid membranes by the adhesion of silica nanoparticles - as seen by neutron spin-echo (NSE)

    Science.gov (United States)

    Hoffmann, Ingo; Michel, Raphael; Sharp, Melissa; Holderer, Olaf; Appavou, Marie-Sousai; Polzer, Frank; Farago, Bela; Gradzielski, Michael

    2014-05-01

    The interactions between nanoparticles and vesicles are of significant interest both from a fundamental as well as from a practical point of view, as vesicles can serve as a model system for cell membranes. Accordingly the effect of nanoparticles that bind to the vesicle bilayer is very important with respect to understanding their biological impact and also may shed some light on the mechanisms behind the effect of nanotoxicity. In this study we have investigated the influence of small adsorbed silica nanoparticles (SiNPs) on the structure of zwitterionic DOPC vesicles. By a combination of SANS, cryo-TEM, and DLS, we observed that the SiNPs are bound to the outer vesicle surface without significantly affecting the vesicle structure. Most interestingly, by means of neutron spin-echo (NSE) local bilayer fluctuations were studied and one finds a small but marked decrease of the membrane rigidity upon binding of the nanoparticles. This surprising finding may be a relevant aspect for the further understanding of the effects that nanoparticles have on phospholipid bilayers.The interactions between nanoparticles and vesicles are of significant interest both from a fundamental as well as from a practical point of view, as vesicles can serve as a model system for cell membranes. Accordingly the effect of nanoparticles that bind to the vesicle bilayer is very important with respect to understanding their biological impact and also may shed some light on the mechanisms behind the effect of nanotoxicity. In this study we have investigated the influence of small adsorbed silica nanoparticles (SiNPs) on the structure of zwitterionic DOPC vesicles. By a combination of SANS, cryo-TEM, and DLS, we observed that the SiNPs are bound to the outer vesicle surface without significantly affecting the vesicle structure. Most interestingly, by means of neutron spin-echo (NSE) local bilayer fluctuations were studied and one finds a small but marked decrease of the membrane rigidity upon

  20. [Factors associated with the consumption of school meals by Brazilian adolescents: results of the PeNSE survey 2012].

    Science.gov (United States)

    Locatelli, Nathália Tarossi; Canella, Daniela Silva; Bandoni, Daniel Henrique

    2017-05-18

    The aim was to study the association between socio-demographic and routine dietary variables and consumption of school meals by adolescents enrolled in public schools in Brazil. The study used data used from the National School Health Survey (PeNSE) 2012. To assess differences between schoolchildren based on whether or not they ate school meals, the study used Pearson's chi-square test, and associations were analyzed with univariate and multivariate Poisson regression models. Of the 86,660 students included in the study, 22.8% eat school meals. Higher consumption of school meals is associated with male gender, brown skin color, residence outside state capitals, working, and low maternal schooling, for those that ate breakfast and lunch with their parents. The findings are relevant for planning strategies to encourage consumption of school meals.

  1. [Socioeconomic status, toothbrushing frequency, and health-related behaviors in adolescents: an analysis using the PeNSE database].

    Science.gov (United States)

    Vettore, Mario Vianna; Moysés, Samuel Jorge; Sardinha, Luciana Monteiro Vasconcelos; Iser, Betine Pinto Moehlecke

    2012-01-01

    This study investigated the association between oral and general health-related behaviors and socioeconomic status, and the relationship between health-related behaviors and toothbrushing among adolescents. The database used here was the National School-Based Health Survey (PeNSE), a cross-sectional population-based study in 2009 with students from 27 Brazilian State capitals. Socio-demographic and health-related behavior data were collected. The survey included 49,189 adolescents (47.5% males), the majority of whom were 14 years of age and enrolled in public schools. The associations between toothbrushing frequency and other health-related behaviors and socioeconomic status varied between boys and girls. Associations were observed between health-related habits and toothbrushing frequency in both sexes, but with variations according to socioeconomic status. Planning health promotion interventions for adolescents should take their individual characteristics and family and social context into account.

  2. The K+ dependent asparaginase, NSE1, is crucial for plant growth and seed production in Lotus japonicus

    DEFF Research Database (Denmark)

    Credali, Alfredo; Garcia-Calderón, Margarita; Dam, Svend Secher

    2013-01-01

    The physiological role of K+-dependent and K+-independent asparaginases in plants remains unclear, and the contribution from individual isoforms during development is poorly understood. We have used reverse genetics to assess the phenotypes produced by the deficiency of K+-dependent NSE1 asparagi...

  3. Effect of MgSO4 on expression of NSE and S-100 in rats brain tissue irradiated by 6 MeV electron beam

    International Nuclear Information System (INIS)

    Zhou Juying; Wang Lili; Yu Zhiying; Qin Songbing; Xu Xiaoting; Li Li; Tu Yu

    2007-01-01

    Objective: To explore the protection of magnesium sulfate (MgSO 4 ) on radiation-induced acute brain injuries. Methods: Thirty six mature Sprague-Dawley rats were randomly divided into 3 groups: blank control group, experimental control group and experimental administered group. The whole brain of SD rats of experimental control group and experimental-therapeutic group were irradiated with a dose of 20 Gy using 6 MeV electron beam. Magnesium sulfate was injected intraperitoneally into the rats of experimental-therapeutic group before and after irradiation for five times. The brain tissue were taken on days 1, 7, 14 and 30 after irradiation. Immunohistochemical method was used to detect the expressions of NSE and S-100 in brain tissue. All data were processed statistically with One-ANOVA analysis. Results: The expressions of NSE and S-100 after whole brain irradiation were time-dependent. Compared with blank control group, the expression of NSE in brains of experimental control group decreased significantly (P 4 can inhibit the expression of S-100, but induce the expression of NSE on radiation-induced acute brain injury. MgSO 4 has a protective effect on radiation-induced acute brain injury. (authors)

  4. Effects of Atorvastatin calcium combined with Aspirin on serum levels of Hcy, NSE, UA, hs-CRP and inflammatory factors of patients with cerebral infarction

    Directory of Open Access Journals (Sweden)

    Shu-Qin Zhang

    2017-03-01

    Full Text Available Objective: To study the effects of Atorvastatin calcium combined with Aspirin on serum levels of homocysteine (Hcy, neuron-specific enolase (NSE, uric acid (UA, high sensitity C-reactive protein (hs-CRP and inflammatory factors of patients with cerebral infarction. Methods: 100 cases of patients with cerebral infarction from March 2014 to May 2016 were treated in the Department of Neurology of our hospital and affiliated to Huazhong University of Science and Technology of traditional Chinese medicine and Western Medicine. The subjects were divided into the control group (n=50 and the treatment group (n=50 randomly. The control group was treated with Aspirin, the treatment group were treated with Atorvastatin calcium combined with Aspirin. The two groups were treated for 28 d. The serum levels of Hcy, NSE, UA, hs- CRP, interleukin-6 (IL-6, interleukin-8 (IL-8 and tumor necrosis factor-α (TNF-α of the two groups before and after treatment were compared. Results: There were no significantly differences of the serum levels of the Hcy, NSE, UA and hs-CRP of the two groups before treatment (P>0.05. After treatment, the serum levels of the Hcy, NSE, UA and hs-CRP of the two groups were significantly lower than before treatment, and that of the treatment group were significantly lower than the control group (P0.05. After treatment, the serum levels of the IL-6, IL-8 and TNF-α of the two groups were significantly lower than before treatment, and that of the treatment group were significantly lower than the control group (P<0.05. Conclusions: Atorvastatin calcium combined with Aspirin can significantly reduce the serum levels of Hcy, NSE, UA, hs-CRP, IL-6, IL-8 and TNF-α of the patients with cerebral infarction.

  5. Clinical significance of determination of serum NSE and plasma ET, IGF-II, CNP levels in patients with acute brain injury

    International Nuclear Information System (INIS)

    Chen Bo

    2010-01-01

    Objective: To investigate the clinical significance of changes of plasma ET, IGF-II, CNP and serum NSE contents in patients with acute brain injury. Methods: Serum contents of neuron specific enolase (NSE) were measured with chemiluminescence immunoassay and plasma endothelin (ET), insulin-like growth factor-II (IGF-II) and C-type natriuretic peptide (CNP) were measured with radioimmunoassay in 30 patients with acute brain injury and 35 controls. Results: Serum contents of NSE and plasma IGF-II, CNP were not much different in patients with mild brain injury from those in controls (P >0.05), but plasma contents of ET were already significantly higher in patients with mild brain injury than those in controls(P < 0.01). The serum NSE and plasma ET levels in patients with moderate and severe brain injury were significantly higher than those in patients with mild brain injury and controls (P < 0.01). Decrease of plasma levels of IGF-II and CNP was not significant in patients with mild brain injury (vs controls). However, the plasma levels of IGF-II and CNP were significantly lower in patients with moderate and severe brain injury than those in patients with mild brain injury and controls (P <0.01). As a whole, the magnitude of changes of these parameters was proportional to the severity of the injury. Conclusion: Changes of serum NSE and plasma IGF-II, ET and CNP levels were closely related to the pathological process of brain injury. Determination of these parameters was of clinical importance for evaluation of the severity of injury and outcome prediction. (authors)

  6. Monitoring carcinogenesis in a case of oral squamous cell carcinoma using a panel of new metabolic blood biomarkers as liquid biopsies.

    Science.gov (United States)

    Grimm, Martin; Hoefert, Sebastian; Krimmel, Michael; Biegner, Thorsten; Feyen, Oliver; Teriete, Peter; Reinert, Siegmar

    2016-09-01

    One of the common malignant tumors of the head and neck worldwide with generally unfavorable prognosis is squamous cell carcinoma (OSCC) of the oral cavity. Early detection of primary, secondary, or recurrent OSCC by liquid biopsy tools is much needed. Twelve blood biomarkers were used for monitoring a case of OSCC suffering from precancerous oral lichen ruber planus mucosae (OLP). After curative R0 tumor resection of primary OSCC (buccal mucosa), elevated epitope detection in monocytes (EDIM)-Apo10, EDIM-transketolase-like-1 (TKTL1), squamous cell carcinoma antigen (SCC-Ag), total serum lactate dehydrogenase (LDH), and its anaerobic isoforms (LDH-4, LDH-5) decreased to normal levels. Three and six months after surgery, transformation of suspicious mucosal lesions has been accompanied with an increase of EDIM scores, total serum LDH values, and a metabolic shift from aerobic (decrease of LDH-1, LDH-2) to anaerobic (increase of LDH-4, LDH-5) conditions. Two months later, secondary OSCC was histopathologically analyzed after tissue biopsy. Cytokeratin fraction 21-1 (CYFRA 21-1), carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) were not affected during the clinical course of carcinogenesis. A combination strategy using a standardized panel of established (metabolic) blood biomarkers (TKTL1, LDH, LDH isoenzymes) is worth and can be recommended among others (apoptosis resistance-related Apo10, SCC-Ag) for early detection and diagnosis of primary, secondary, and recurrent OSCC. A tandem strategy utilizing (metabolic pronounced) routine liquid biopsies with imaging techniques may enhance diagnosis of OSCC in the future. Although we demonstrated the diagnostic utility of separated liquid biopsies in our previous study cohorts, further investigations in a larger patient cohort are necessary to recommend this combination strategy (EDIM blood test, LDH value, metabolic shift of LDH isoenzymes, and others, e.g., SCC-Ag or immunophenotyping) as a

  7. [Utility of Multiple Increased Lung Cancer Tumor Markers in Treatment of Patients with Advanced Lung Adenocarcinoma].

    Science.gov (United States)

    Peng, Yan; Wang, Yan; Hao, Xuezhi; Li, Junling; Liu, Yutao; Wang, Hongyu

    2017-10-20

    Among frequently-used tumor markers in lung cancer, carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA125), cytokeratin 19 (CYFRA21-1) and squamous carcinoma antigen (SCC), neuron specific enolase (NSE) and pro-gastrin-releasing peptide (ProGRP) are respectively expressed highly in lung adenocarcinoma, lung squamous carcinoma and small cell lung cancer. By comparing patients with multiple increased tumor markers (group A) and patients with increase of CEA and/or CA125 (group B), this study aims to investigate the utility of multiple increased tumor markers in therapeutic evaluation and prediction of disease relapsing in patients with advanced lung adenocarcinoma. Patients with stage IV lung adenocarcinoma who receiving the first line chemotherapy in Cancer Hospital, Chinese Academy of Medical Sciences were enrolled and retrospectively analyzed. Clinical characteristic, serum tumor markers before chemotherapy, efficacy evaluation, progression-free survival (PFS) were analyzed. Except CEA and CA125, the highest ratio of increased tumor markersin group A was CYFRA21-1 (93%), then was NSE (36%), SCC (13%) and ProGRP (12%). Patients with multiple increased tumor markers tend to have more distant metastasis (Ptumor markers have high risk of relapse, and maintenance therapy can reduce relapse risk.

  8. Analysis of relationship between tumor markers and quantification of free DNA in serum of lung cancer patients

    International Nuclear Information System (INIS)

    Yang Shunfang; Zhang Peiling; Cao Jie; Zeng Jun; Dong Qianggang

    2006-01-01

    To evaluate the diagnostic value and relationship between five tumor markers (CA19- 9,CA125,CYFRA21-1 ,CEA,NSE) and free DNA in serum for lung cancer detection and try to find a new and more efficient tumor marker, the amounts of CA19-9, CA125, CYFRA21-1, CEA, NSE were determined by RIA and free DNA was determined by the use of quantitative real time PCR amplification of the human epidermal growth factor receptor (EGFR) in 52 lung cancer patients and 8 cases of benign pulmonary disease and 10 healthy controls. The resulls showed that average concentration of free DNA in serum of lung cancer patients, benign pulmo- nary disease and healthy controls was 107.6ng/mL, 76.86ng/mL and 18.8ng/mL, respective- ly. The diagnostic sensitivity, specificity and accuracy of free DNA for lung cancer were 71. 2%, 50% and 68.3%, same as the diagnostic value of combined detection of five tumor markers. The sensitivity, specificity and accuracy of the five tumor markers and free DNA combinend detection for lung cancer were 94.2%, 25% and 85%, respectively. The free DNA in the serum of lung cancer patients may be a new and better tumor marker. (authors)

  9. Clinical Evaluation and Cost-Effectiveness Analysis of Serum Tumor Markers in Lung Cancer

    Directory of Open Access Journals (Sweden)

    Rong Wang

    2013-01-01

    Full Text Available The detection of serum tumor markers is valuable for the early diagnosis of lung cancer. Tumor markers are frequently used for the management of cancer patients. However, single markers are less efficient but marker combinations increase the cost, which is troublesome for clinics. To find an optimal serum marker combination panel that benefits the patients and the medical management system as well, four routine lung cancer serum markers (SCCA, NSE, CEA, and CYFRA21-1 were evaluated individually and in combination. Meanwhile, the costs and effects of these markers in clinical practice in China were assessed by cost-effectiveness analysis. As expected, combinations of these tumor markers improved their sensitivity for lung cancer and different combination panels had their own usefulness. NSE + CEA + CYFRA21-1 was the optimal combination panel with highest Youden’s index (0.64, higher sensitivity (75.76%, and specificity (88.57%, which can aid the clinical diagnosis of lung cancer. Nevertheless, the most cost-effective combination was SCCA + CEA, which can be used to screen the high-risk group.

  10. Clinical significance of combined determination of serum neuron-specific enolase (NSE), tumor necrosis factor-α (TNF-α) and lipid-associated sialic acid (LSA) in patients with lung cancer

    International Nuclear Information System (INIS)

    Wu Wei; Yao Dengfu; Qiu Liwei; Wu Xinghua

    2003-01-01

    Objective: To explore the expression and the diagnostic value of determining serum neuron-specific enolase (NSE), tumor necrosis factor-α (TNF-α) and lipid-associated sialic acid (LSA) in patients with lung cancer. Methods: The concentrations of NSE, TNF-α and LSA were measured in 78 patients with lung cancer and 32 patients with benign lung diseases as well as 109 controls by enzymelinked immunosorbent assay (ELISA) and chemical assay respectively. Results: The levels of NSE (19.78 ± 12.10 ng/ml), TNF-α (135.64 ± 49.01 pg/ml) and LSA (106 ± 0.31 ng/ml) were significantly higher in patients with lung cancer than those in patients with benign lung diseases (NSE 7.56 ± 3.41 ng/ml, TNF-α 84.70 ± 24.89 pg/ml, LSA 0.78 ± 0.18 mg/ml) and controls (NSE 8.01 ± 2.81 ng/ml, TNF-α 71.25 ± 13.50 pg/ml, LSA 0.70 ± 0.13 ng/ml) (all p < 0.01). Conclusion: The present data suggest that the syntheses of NSE, TNF-α and LSA increase in patients with lung cancer and combined determination of NSE, TNF-α and LSA be helpful to diagnosis of lung cancer

  11. Consumption of sugar-rich food products among Brazilian students:National School Health Survey (PeNSE 2012

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    Nathália Luíza Ferreira

    2015-12-01

    Full Text Available Abstract This study aimed to analyze the consumption of high-sugar foods by Brazilian schoolchildren and to identify associated factors, based on data from the National School Health Survey (PeNSE 2012. Consumption of these foods was classified as: do not consume sweets and soft drinks regularly; consume sweets or soft drinks regularly; and consume sweets and soft drinks regularly. Its association with sociodemographic information, eating habits, and family contexts were investigated via multiple ordinal regressions. Regular consumption of sweets and/or soft drinks was reported by 19.2% and 36.1% of adolescents, respectively, and higher prevalence was associated with female gender, age 14-15 years, higher maternal education, not living with the mother and father, not eating meals with the parents, eating while watching TV, and longer TV time. Nearly one-fifth of adolescents regularly consumed sweets and soft drinks, which was associated with socio-demographic and behavioral factors that should be targeted in order to improve their food consumption.

  12. Transfer Learning for SSVEP Electroencephalography Based Brain–Computer Interfaces Using Learn++.NSE and Mutual Information

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    Matthew Sybeldon

    2017-01-01

    Full Text Available Brain–Computer Interfaces (BCI using Steady-State Visual Evoked Potentials (SSVEP are sometimes used by injured patients seeking to use a computer. Canonical Correlation Analysis (CCA is seen as state-of-the-art for SSVEP BCI systems. However, this assumes that the user has full control over their covert attention, which may not be the case. This introduces high calibration requirements when using other machine learning techniques. These may be circumvented by using transfer learning to utilize data from other participants. This paper proposes a combination of ensemble learning via Learn++ for Nonstationary Environments (Learn++.NSEand similarity measures such as mutual information to identify ensembles of pre-existing data that result in higher classification. Results show that this approach performed worse than CCA in participants with typical SSVEP responses, but outperformed CCA in participants whose SSVEP responses violated CCA assumptions. This indicates that similarity measures and Learn++.NSE can introduce a transfer learning mechanism to bring SSVEP system accessibility to users unable to control their covert attention.

  13. Factors associated with injuries in adolescents, from the National Adolescent School-based Health Survey (PeNSE 2012

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    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To estimate the prevalence of injuries among teenagers and to examine the associated risk factors, such as sociodemographic characteristics, risk behaviors, family ties and other factors. METHOD: The prevalence of the outcome (injury was estimated with a 95%confidence interval. In order to verify factors associated with the injury, a bivariate analysis was made with estimated odds ratio (OR and its respective confidence intervals. Then, a multivariate analysis was carried out, only with variables whose descriptive level was equal to or lower than 5% (p < 0.05 remaining in the model. RESULTS: The study of injury in adolescents, based on the data from the National Adolescent School-based Health Survey (PeNSE, pointed out that 10.3% of the teenagers suffered severe injuries in the past 12 months, such as cuts or perforations, broken bones or dislocated joints. The following variables remained independently associated with "suffering severe injuries": being a male teenager; black, mulatto or indigenous race/color and working. Factors related to family ties are significant when the relations are fragile amongst members: adolescents that are injured the most are the ones who suffer most aggressions at home, who skip classes without notifying their parents, those who do not live with their parents and have low family control. The most relevant aspects of mental health are insomnia and loneliness. The factors associated to the exposure to situations of violence that remained in the model were: insecurity in school and in the route home-school; getting a ride with someone inebriated; drinking and driving motorized vehicles; not wearing the seatbelt; not wearing a helmet and being bullied. Among the factors of individual behavior, the following can be emphasized: use of alcohol, cigarettes, trying illicit drugs and early sexual intercourse. CONCLUSION: The analysis of the determinants for suffering injuries in childhood and adolescence shows

  14. Lifetime use of illicit drugs and associated factors among Brazilian schoolchildren, National Adolescent School-based Health Survey (PeNSE 2012)

    OpenAIRE

    Rogério Lessa Horta; Bernardo Lessa Horta; Andre Wallace Nery da Costa; Rogério Ruscitto do Prado; Maryane Oliveira-Campos; Deborah Carvalho Malta

    2014-01-01

    OBJECTIVE: This study aimed at describing the prevalence of illicit drug use among 9th grade students in the morning period of public and private schools in Brazil, and assessing associated factors. METHOD: The Brazilian survey PeNSE (National Adolescent School-based Health Survey) 2012 evaluated a representative sample of 9th grade students in the morning period, in Brazil and its five regions. The use of illicit drugs at least once in life was assessed for the most commonly used drugs,...

  15. Efficiency of S&P CNX Nifty Index Option of the National Stock Exchange (NSE, India, using Box Spread Arbitrage Strategy

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    G. P. Girish

    2014-09-01

    Full Text Available Box spread is a trading strategy in which one simultaneously buys and sells options having the same underlying asset and time to expiration, but different exercise prices. This study examined the efficiency of European style S&P CNX Nifty Index options of National Stock Exchange, (NSE India by making use of high-frequency data on put and call options written on Nifty (Time-stamped transactions data for the time period between 1st January 2002 and 31st December 2005 using box-spread arbitrage strategy. The advantages of box-spreads include reduced joint hypothesis problem since there is no consideration of pricing model or market equilibrium, no consideration of inter-market non-synchronicity since trading box spreads involve only one market, computational simplicity with less chances of mis-specification error, estimation error and the fact that buying and selling box spreads more or less replicates risk-free lending and borrowing. One thousand three hundreds and fifty eight exercisable box-spreads were found for the time period considered of which 78 Box spreads were found to be profitable after incorporating transaction costs (32 profitable box spreads were identified for the year 2002, 19 in 2003, 14 in 2004 and 13 in 2005 The results of our study suggest that internal option market efficiency has improved over the years for S&P CNX Nifty Index options of NSE India.

  16. Brain injury markers (S100B and NSE in chronic cocaine dependents Marcadores de lesão cerebral (S100B e NSE em dependentes crônicos de cocaína

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    Felix Henrique Paim Kessler

    2007-06-01

    Full Text Available OBJECTIVE: Studies have shown signs of brain damage caused by different mechanisms in cocaine users. The serum neuron specific enolase and S100B protein are considered specific biochemical markers of neuronal and glial cell injury. This study aimed at comparing blood levels of S100B and NSE in chronic cocaine users and in volunteers who did not use cocaine or other illicit drugs. METHOD: Twenty subjects dependent on cocaine but not on alcohol or marijuana, and 20 non-substance using controls were recruited. Subjects were selected by consecutive and non-probabilistic sampling. Neuron specific enolase and S100B levels were determined by luminescence assay. RESULTS: Cocaine users had significantly higher scores than controls in all psychiatric dimensions of the SCL-90 and had cognitive deficits in the subtest cubes of WAIS and the word span. Mean serum S100B level was 0.09 ± 0.04 µg/l among cocaine users and 0.08 ± 0.04 µg/l among controls. Mean serum neuron specific enolase level was 9.7 ± 3.5 ng/l among cocaine users and 8.3 ± 2.6 ng/l among controls. CONCLUSIONS: In this first study using these specific brain damage markers in cocaine users, serum levels of S100B and neuron specific enolase were not statistically different between cocaine dependent subjects and controls.OBJETIVO: Estudos têm demonstrado sinais de lesão cerebral causadas por diferentes mecanismos em usuários de cocaína. A enolase sérica neurônio-específica e a proteína S100B são consideradas marcadores bioquímicos específicos de lesão neuronal e glial. Este estudo objetivou comparar os níveis sangüíneos de S100B e enolase sérica neurônio-específica em usuários crônicos de cocaína e em voluntários que não usam cocaína ou outras drogas ilícitas. MÉTODO: Vinte sujeitos dependentes de cocaína, mas não dependentes de álcool, maconha ou outra droga, e 20 sujeitos controles não usuários de drogas foram recrutados. Os sujeitos foram selecionados por

  17. [Sedentary leisure time and food consumption among Brazilian adolescents: the Brazilian National School-Based Adolescent Health Survey (PeNSE), 2009].

    Science.gov (United States)

    Camelo, Lidyane do Valle; Rodrigues, Jôsi Fernandes de Castro; Giatti, Luana; Barreto, Sandhi Maria

    2012-11-01

    The objective of this paper was to investigate whether sedentary leisure time was associated with increased regular consumption of unhealthy foods, independently of socio-demographic indicators and family context. The analysis included 59,809 students from the Brazilian National School-Based Adolescent Health Survey (PeNSE) in 2009. The response variable was sedentary leisure time, defined as watching more than two hours of TV daily. The target explanatory variables were regular consumption of soft drinks, sweets, cookies, and processed meat. Odds ratios (OR) and 95% confidence limits (95%CI) were obtained by multiple logistic regression. Prevalence of sedentary leisure time was 65%. Regular consumption of unhealthy foods was statistically higher among students reporting sedentary leisure time, before and after adjusting for sex, age, skin color, school administration (public versus private), household assets index, and household composition. The results indicate the need for integrated interventions to promote healthy leisure-time activities and healthy eating habits among young people.

  18. The serum levels of tumor marker CA19-9, CEA, CA72-4, and NSE in type 2 diabetes without malignancy and the relations to the metabolic control.

    Science.gov (United States)

    Shang, Xiaojing; Song, Chunqing; Du, Xiaoming; Shao, Hailin; Xu, Donghong; Wang, Xiaolai

    2017-02-01

    To investigate whether there is a difference in carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen (CEA), carbohydrate antigen 72-4 (CA72-4), and neuron-specific enolase (NSE) between diabetic and non-diabetic patients.  Methods: A retrospective analysis was performed in 268 type 2 diabetic patients and 95 non-diabetic ones, and their serum levels of CA19-9, CEA, CA72-4, and NSE were compared in our endocrine ward at the Tianjin Fourth Central Hospital, Tianjin, Chinaduring the period from January to June 2015. The diabetic patients were divided into 4 groups based on glycosylated hemoglobin (HbA1c) levels to investigate the relationship between levels of tumor markers and glucose status.  Results: Diabetic patients had higher levels of tumor markers than non-diabetic subjects (CA19-9: 13.0 versus 7.25U/mL, p=0.000; CEA: 2.55 versus 2.25 ng/mL, p=0.012; CA72-4: 1.95 versus 1.50U/mL, p=0.001; NSE: 11.64 versus 10.22ng/mL, p=0.000). CA19-9 levels increased in a stepwise manner with poor diabetes status. CEA levels were increased in patients with HbA1c ≥9% and CA72-4 elevation was predominant in patients with poor glycemic control (HbA1c ≥11%). NSE levels were not associated with metabolic parameters.  Conclusion: Serum levels of CA19-9, CEA, CA72-4, and NSE were elevated in type 2 diabetes; however, only CA19-9, CEA, and CA72-4 levels were associated with hyperglycemia.

  19. Effects of donepezil combined with folic acid and vitamin B12 on serum levels of inflammatory factors, HCY, NSE and neurotransmitters in elderly patients with Alzheimer' s disease complicated with hyperhomocysteinemia

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    Fang-Fang Zheng

    2017-08-01

    Full Text Available Objective: To investigate the effects of donepezil combined with folic acid and vitamin B12 on the levels of serum inflammatory factors, Hcy, NSE and neurotransmitters in elderly patients with Alzheimer's disease (AD complicated with hyperhomocysteinemia. Methods: A total of 98 elderly patients with AD complicated with hyperhomocysteinemia were randomly divided into control group (n=48 and observation group (n=50 according to the random data table method. Patients in the control group were treated with donepezil. On this basis, the patients in the observation group were treated with folic acid and vitamin B12, all patients were treated for 3 months. Before and after treatment, the levels of serum inflammatory factors (TNF-α, IL-6 and hs-CRP, Hcy, NSE and brain neurotransmitter (5-HT, NE and DA were compared between the two groups. Results: Before treatment, the levels of TNF-α, IL-6, hs-CRP, Hcy, NSE, 5-HT, NE and DA of the control group and the observation group were not statistically significant. Compared with the group before treatment, the levels of TNF-α, IL-6, hs-CRP, Hcy and NSE in the two groups were significantly lower, and the level of the observation group was significantly lower than those of the control group, the difference was statistically significant; Compared with the group before treatment, the levels of 5-HT, NE and DA in the two groups were significantly increased, and the observation group was significantly higher than that of the control group, the difference was statistically significant. Conclusion: Donepezil combined folic acid and vitamin B12 in treatment of AD with hyperhomocysteinemia, which can effectively reduce the body's inflammatory response, reduced Hcy and NSE levels, elevated levels of brain neurotransmitters, has important clinical significance.

  20. The serum levels of tumor marker CA19-9, CEA, CA72-4, and NSE in type 2 diabetes without malignancy and the relations to the metabolic control

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    Chunqing Song

    2017-02-01

    Full Text Available Objectives: To investigate whether there is a difference in carbohydrate antigen 19-9 (CA19-9, carcinoembryonic antigen (CEA, carbohydrate antigen 72-4 (CA72-4, and neuron-specific enolase (NSE between diabetic and non-diabetic patients. Methods: A retrospective analysis was performed in 268 type 2 diabetic patients and 95 non-diabetic ones, and their serum levels of CA19-9, CEA, CA72-4, and NSE were compared in our endocrine ward at the Tianjin Fourth Central Hospital, Tianjin, China during the period from January to June 2015. The diabetic patients were divided into 4 groups based on glycosylated hemoglobin (HbA1c levels to investigate the relationship between levels of tumor markers and glucose status. Results: Diabetic patients had higher levels of tumor markers than non-diabetic subjects (CA19-9: 13.0 versus 7.25U/mL, p=0.000; CEA: 2.55 versus 2.25 ng/mL, p=0.012; CA72-4: 1.95 versus 1.50U/mL, p=0.001; NSE: 11.64 versus 10.22ng/mL, p=0.000. CA19-9 levels increased in a stepwise manner with poor diabetes status. CEA levels were increased in patients with HbA1c ≥9% and CA72-4 elevation was predominant in patients with poor glycemic control (HbA1c ≥11%. NSE levels were not associated with metabolic parameters. Conclusion: Serum levels of CA19-9, CEA, CA72-4, and NSE were elevated in type 2 diabetes; however, only CA19-9, CEA, and CA72-4 levels were associated with hyperglycemia.

  1. Differential diagnosis of a solitary pulmonary nodule of the lung on the grounds of selected laboratory tests and radiological examination

    International Nuclear Information System (INIS)

    Szlachcinska, A.; Kozak, J.

    2011-01-01

    Objective: To present in detail the diagnosis of solitary pulmonary nodule and especially evaluation of: clinical data, analysis of radiological images, selected laboratory tests. Material and methods: There were 50 patients - 31 men and 19 women at the mean age 58.7 ± 11.4 years old who underwent surgical treatment because of a solitary pulmonary nodule. Interview, physical examination, computed tomography, bronchoscopy, spirometry, and laboratory tests needed for the operation were performed in all these patients. Additionally LDH, fibrinogen, ESR, and the tumour markers CEA, Ca 15-3, Ca 19-9, NSE, SCC, and Cyfra 21-1 were measured from the blood sample collected during admission. Results: Malignant tumour was diagnosed in 24 patients, benign in 26. There is a significant difference between patients with malignant and nonmalignant tumours in age (54.46 years vs. 63.33 years), size of the tumour in the lung scan of chest CT (1.53 cm vs. 1.91 cm) and location (lower right lobe vs. upper right lobe). There is no significant difference between type of tumour and sex, clinical symptoms and laboratory tests. Conclusions: 1. The risk factors of malignancy in patient with solitary pulmonary nodule are: age ≥ 56.5 years, size of the tumour in the lung scan of chest CT ≥ 1.45 cm, location in upper right lobe. 2. LDH, fibrinogen, ESR, and the tumour markers CEA, Ca 15-3, Ca 19-9, NSE, SCC, and Cyfra 21-1 are not useful in differential diagnosis of solitary pulmonary nodule. (authors)

  2. meta-analysis of Serum Tumor Markers in Lung Cancer

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    Xianfeng LU

    2010-12-01

    Full Text Available Background and objective The detection of serum tumor markers is of great value for early diagnosis of lung cancer. The aim of this study is to summarize the clinic significance characteristics of serum markers contributing to the detection of lung cancer. Methods References about serum markers of lung cancer were estimated using meta-analysis method. 712 references which included more than 20 cases, 20 controls, the serum markers of 52 832 patients with malignancies and 32 037 patients as controls were evaluated. Results Overall the detection of 13 markers play a significant part in lung cancer diagnosis. The sensitivity of CEA, CA125, CYFRA21-1, TPA, SCCAg, DKK1, NSE, ProGRP in the patients’ serum with lung cancer were 47.50%, 50.11%, 57.00%, 50.93%, 49.00%, 69.50%, 39.73%, 51.48% and the specificity were 92.34%, 80.19%, 90.16%, 88.41%, 91.07%, 92.20%, 89.11%, 94.89%. In the combined analysis of tumor markers: the sensitivity, specificity of NSE+ProGRP were 88.90% and 72.82% in diagnosis of small cell lung cancer, respectively. In diagnosis of squamous corcinoma, the sensitivity and specificity of TSGF+SCCAg+CYFRA21-1 were 95.30% and 74.20%. The the sensitivity and specificity of CA153+Ferrtin+CEA were 91.90% and 44.00% in diagnosis of lung cancer. Conclusion Although the assay of tumor markers in serum is useful for diagnosis of early lung cancer, the sensitivity and specificity are low. Combined detection of these tumor markers could increase sensitivity and specificity.

  3. Diablo 2.0: A modern DNS/LES code for the incompressible NSE leveraging new time-stepping and multigrid algorithms

    Science.gov (United States)

    Cavaglieri, Daniele; Bewley, Thomas; Mashayek, Ali

    2015-11-01

    We present a new code, Diablo 2.0, for the simulation of the incompressible NSE in channel and duct flows with strong grid stretching near walls. The code leverages the fractional step approach with a few twists. New low-storage IMEX (implicit-explicit) Runge-Kutta time-marching schemes are tested which are superior to the traditional and widely-used CN/RKW3 (Crank-Nicolson/Runge-Kutta-Wray) approach; the new schemes tested are L-stable in their implicit component, and offer improved overall order of accuracy and stability with, remarkably, similar computational cost and storage requirements. For duct flow simulations, our new code also introduces a new smoother for the multigrid solver for the pressure Poisson equation. The classic approach, involving alternating-direction zebra relaxation, is replaced by a new scheme, dubbed tweed relaxation, which achieves the same convergence rate with roughly half the computational cost. The code is then tested on the simulation of a shear flow instability in a duct, a classic problem in fluid mechanics which has been the object of extensive numerical modelling for its role as a canonical pathway to energetic turbulence in several fields of science and engineering.

  4. Lifetime use of illicit drugs and associated factors among Brazilian schoolchildren, National Adolescent School-based Health Survey (PeNSE 2012).

    Science.gov (United States)

    Horta, Rogério Lessa; Horta, Bernardo Lessa; da Costa, Andre Wallace Nery; do Prado, Rogério Ruscitto; Oliveira-Campos, Maryane; Malta, Deborah Carvalho

    2014-01-01

    This study aimed at describing the prevalence of illicit drug use among 9th grade students in the morning period of public and private schools in Brazil, and assessing associated factors. The Brazilian survey PeNSE (National Adolescent School-based Health Survey) 2012 evaluated a representative sample of 9th grade students in the morning period, in Brazil and its five regions. The use of illicit drugs at least once in life was assessed for the most commonly used drugs, such as marijuana, cocaine, crack, solvent-based glue, general ether-based inhalants, ecstasy and oxy. Data were subjected to descriptive analysis, and Pearson's χ² test and logistic regression was used in the multivariate analysis. The use of illicit drugs at least once in life was reported by 7.3% (95%CI 5.3 - 9.4) of the respondents. Logistic regression was used for multivariate analysis and the evidences suggest that illicit drug use is associated to social conditions of greater consumption power, the use of alcohol and tobacco, behaviors related to socialization, such as having friends or sexual activity, and also the perception of loneliness, loose contact between school and parents and experiences of abuse in the family environment. The outcome was inversely associated with close contact with parents and parental supervision. In addition to the association with the processes of socialization and consumption, the influence of family and school is expressed in a particularly protective manner in different records of direct supervision and care.

  5. Inconsistent reports of risk behavior among Brazilian middle school students: National School Based Survey of Adolescent Health (PeNSE 2009/2012

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    Dandara de Oliveira Ramos

    Full Text Available Abstract: This study assessed the consistency of self-reports of risk behavior (overall and within four specific domains: alcohol use, tobacco use, drug use, and sexual activity in two editions of the Brazilian National School Based Survey of Adolescent Health (PeNSE: 2009 and 2012. The overall proportion of cases with at least one inconsistent response in the two editions was 11.7% (2.7% on the alcohol items, 2.1% for drug use, 4.3% for cigarette use, 3% for sexual activity and 22.7% (12.8% on alcohol items, 2.5% for drug use, 4.3% for cigarette use, 4.1% for sexual activity, respectively. Such inconsistency was more prevalent among males, delayed students, those who reported having experimented with drugs, and those who did not have a cellphone. Because inconsistent responses were more prevalent among the students who claimed to have engaged in risky activities, removing inconsistent responders affected the estimated prevalence of all risk behaviors in both editions of the survey. This study supports the importance of performing consistency checks of self-report surveys, following the growing body of literature on this topic.

  6. Saúde sexual e reprodutiva de adolescentes: interlocuções com a Pesquisa Nacional de Saúde do Escolar (PeNSE

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    Helena Maria Campos

    Full Text Available Este artigo analisa a saúde sexual e reprodutiva de adolescentes, com base em resultados da PeNSE, a partir de três variáveis comparativas: a dependência administrativa (escolas públicas e privadas; b sexo; c localidade (Brasil e Belo Horizonte, utilizando proporções e intervalos de confiança de 95% (IC95%. A proporção de escolares que já tiveram relação sexual (30,5% é maior nas escolas públicas do que nas privadas, ocorrendo mais cedo e em dobro para o sexo masculino. Entre os entrevistados, o preservativo não foi utilizado por 24,1% na ultima relação sexual, sem diferenças entre sexos e tipo de escola. Constata-se, assim, a necessidade de políticas públicas e compromisso com a promoção da saúde sexual e reprodutiva na adolescência.

  7. Utilidade dos marcadores tumorais no acompanhamento de doentes com carcinoma brônquico

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    C. Robalo Cordeiro

    1995-07-01

    Full Text Available RESUMO: Os autores descrevem dois estudos de Marcadores Tumorais realizados no lnstituto de Patologia Geral da Faculdade de Medicina de Coimbra, em colaboração com o Serviço de Pneumologia dos HUC.Num primeiro trabalho, em que se dosearam CEA, NSE, SCC, CA19.9 e CA125, em 58 doentes com Carcinoma Brônquico (CB, em 29 índivíduos saudáveis e em 15 doentes com Tuberculose Pulmonar (TP, apenas se realizaram estudos de especificidade e de sensibilidade (isolada e em paineis de marcadores.No segundo trabalho realizaram-se doseameotos de CEA, NSE, TPS, CYFRA 21.1., e SCC, no soro, em líquidos de lavagem broocoalveolar e em líquidos pleurais, em 24 doentes com CB, em 9 doentes com Doença Pulmonar Obstrutiva Crónica (DPOC, em 8 doentes com TP e em 14 indivíduos saudáveis; acompanhou-se 12 doentes em terapêutica citostática e 4 doentes submetidos a intervenção cirúrgica.Dos resultados obtidos salientam-se diferenças significativas nos valores obtidos, com o TPS, no soro, comparando os doentes com CB, quer com todo o grupo controlo (DPOC e saudáveis,- p=0.007 -, quer apenas com os indivíduos saudáveis - p=0.0007 -; na comparação dos níveis séricos dos doentes com DPOC com os dos indivíduos saudáveis, igualmente se registaram diferenças significativas para o TPS- p=0.002 -, bern como para o CEA- p=0.0004 - e para o CYFRA 21.1.- p=0.04.Das correlaçõoes histopatológicas, apenas o CEA revelou níveis significativamente elevados- p=0.02- em adenocarcinomas, relativamente aos outros tipos.Verificaram-se diferenças significativas, para os doentes come sem metástases a distância, nos valores de CEA- p=0.01 -,bern como, no que diz respeito ao TPS, nos doentes estadiados ate ao grupo IIIa, relativamente aos estadios IIIb e IV -p=0.02-. Do acompanhamento dos doentes em quimioterapia e cirurgia, retiram-se úteis sugestões, que se discutem.Relativamente aos estudos nos líquidos de Lavagem broncoalveolar, e embora se verifiquem

  8. Sociodemographic inequalities in leisure-time physical activity and active commuting to school in Brazilian adolescents: National School Health Survey (PeNSE 2009, 2012, and 2015).

    Science.gov (United States)

    Ferreira, Rodrigo Wiltgen; Varela, Andrea Ramirez; Monteiro, Luciana Zaranza; Häfele, César Augusto; Santos, Simone José Dos; Wendt, Andrea; Silva, Inácio Crochemore Mohnsam

    2018-01-01

    The objective of this study was to identify inequalities in leisure-time physical activity and active commuting to school in Brazilian adolescents, as well as trends according to gender, type of school, maternal schooling, and geographic region, from 2009 to 2015. This was a descriptive study based on data from the Brazilian National School Health Survey (PeNSE) in 2009, 2012, and 2015. Students were defined as active in their leisure time when they practiced at least 60 minutes of physical activity a day on five or more of the seven days prior to the interview. Active commuting to school was defined as walking or biking to school on the week prior to the interview. The outcomes were stratified by gender, type of school, maternal schooling, and geographic region. Inequalities were assessed by differences and ratios between the estimates, as well as summary inequality indices. The 2009, 2012, and 2015 surveys included 61,301, 61,145, and 51,192 schoolchildren, respectively. Prevalence of leisure-time physical activity was 13.8% in 2009, 15.9% in 2012, and 14.7% in 2015; the rates for active commuting to school were 70.6%, 61.7%, and 66.7%, respectively. Boys showed 10 percentage points higher prevalence of leisure-time physical activity and 5 points higher active commuting to school than girls. Children of mothers with more schooling showed a mean of 10 percentage points higher prevalence of leisure-time physical activity than children of mothers with the lowest schooling and some 30 percentage points lower in relation to active commuting to school. The observed inequalities remained constant over the course of the period. The study identified socioeconomic and gender inequalities that remained constant throughout the period and which were specific to each domain of physical activity.

  9. Dor dentária e fatores associados em adolescentes brasileiros: a Pesquisa Nacional de Saúde do Escolar (PeNSE, Brasil, 2009 Dental pain and associated factors in Brazilian adolescents: the National School-Based Health Survey (PeNSE, Brazil, 2009

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    Maria do Carmo Matias Freire

    2012-01-01

    Full Text Available O objetivo deste estudo foi estimar a prevalência da dor de dente em adolescentes brasileiros e analisar fatores sociodemográficos e comportamentais associados, utilizando os dados da Pesquisa Nacional de Saúde do Escolar (PeNSE de 2009. A pesquisa foi realizada pelo Instituto Brasileiro de Geografia e Estatística e pelo Ministério da Saúde em escolares com idades entre 11 e 17 anos ou mais, das 27 capitais brasileiras, por meio de questionário autoaplicável. Utilizou-se a análise de regressão de Poisson, segundo um modelo hierárquico de determinação. A prevalência de dor na amostra (n = 54.985 nos últimos seis meses foi de 17,8% (IC95%: 17,5-18,1. Prevalências mais elevadas foram encontradas em mulheres, naqueles com 14 anos ou mais, das raças preta, parda e indígena, de escolas públicas, cujas mães tinham baixa escolaridade, que não moravam com a mãe, que haviam experimentado cigarro e álcool alguma vez na vida, que relataram menor frequência de escovação e maior consumo de guloseimas e refrigerantes. A prevalência de dor foi considerável e associada a aspectos sociodemográficos e de comportamentos relacionados à saúde.The aim of this study was to assess the prevalence of dental pain and associated socio-demographic and behavioral factors in Brazilian adolescents, using data from the National School-Based Health Survey (PeNSE, Brazil, 2009. The survey was conducted by the Brazilian Institute of Geography and Statistics (IBGE and Ministry of Health in students 11 to 17 years of age or older in the 27 State capitals, using a self-administered questionnaire. Analyses included Poisson regression following a hierarchical approach. Prevalence of dental pain in the sample (n = 54,985 in the previous six months was 17.8% (95%CI: 17.5-18.1. Higher prevalence was associated with female gender, age 14 years and over, racial self-identification as black, brown, or indigenous, enrollment in public schools, lower maternal

  10. Lazer sedentário e consumo de alimentos entre adolescentes brasileiros: Pesquisa Nacional de Saúde do Escolar (PeNSE, 2009 Sedentary leisure time and food consumption among Brazilian adolescents: the Brazilian National School-Based Adolescent Health Survey (PeNSE, 2009

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    Lidyane do Valle Camelo

    2012-11-01

    Full Text Available O objetivo deste trabalho foi investigar se o lazer sedentário está associado a um maior consumo regular de alimentos não saudáveis independentemente de indicadores sociodemográficos e de contexto familiar. A análise envolveu 59.809 escolares da Pesquisa Nacional de Saúde do Escolar (PeNSE conduzida em 2009. A variável resposta foi o lazer sedentário, definido como o tempo diário em frente à TV superior a duas horas/dia. As variáveis explicativas de interesse foram o consumo regular de refrigerantes, guloseimas, biscoitos doces e embutidos. Odds ratios (OR e intervalos de 95% de confiança (IC95% foram obtidos por regressão logística múltipla. A prevalência de lazer sedentário foi de 65%. O consumo regular de alimentos não saudáveis foi estatisticamente maior entre os que relataram lazer sedentário, antes e após ajuste por sexo, idade, cor da pele autorreferida, dependência administrativa da escola, índice de bens no domicílio e composição familiar. Esses resultados apontam a necessidade de intervenções que promovam, de forma integrada, lazer e dieta saudável entre os jovens.The objective of this paper was to investigate whether sedentary leisure time was associated with increased regular consumption of unhealthy foods, independently of socio-demographic indicators and family context. The analysis included 59,809 students from the Brazilian National School-Based Adolescent Health Survey (PeNSE in 2009. The response variable was sedentary leisure time, defined as watching more than two hours of TV daily. The target explanatory variables were regular consumption of soft drinks, sweets, cookies, and processed meat. Odds ratios (OR and 95% confidence limits (95%CI were obtained by multiple logistic regression. Prevalence of sedentary leisure time was 65%. Regular consumption of unhealthy foods was statistically higher among students reporting sedentary leisure time, before and after adjusting for sex, age, skin color

  11. Aplicação clínica dos marcadores tumorais séricos em carcinoma não-pequenas células do pulmão

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    GROSS JEFFERSON LUIZ

    2000-01-01

    Full Text Available O valor clínico dos marcadores tumorais séricos em câncer de pulmão é incerto. Objetivos: Avaliar a associação da concentração sérica de marcadores tumorais com a extensão da neoplasia e seu valor prognóstico. Casuística e método: Entre fevereiro de 1995 e setembro de 1997 foram estudados 103 pacientes, no Departamento de Cirurgia Torácica do Hospital do Câncer. Antes do tratamento os pacientes foram submetidos à coleta de sangue para dosagem da concentração do CEA, CYFRA21.1, CA15.3, CA19.9, CA72.4 e NSE. Resultados: O CYFRA21.1 foi o marcador mais freqüentemente elevado (55%. Os pacientes com neoplasia avançada tiveram concentração sérica média do CEA (90,82 ± 329,08ng/ml, CYFRA21.1 (20,34 ± 58,42ng/ml e CA15.3 (56,54 ± 86,81U/ml significativamente superior às observadas nos tumores localizados, respectivamente, 10,24 ± 35,96ng/ml, 12,67 ± 25,23ng/ml e 22,22 ± 15,86U/ml. Mesmo considerando todos os marcadores deste estudo, apenas os pacientes com CEA elevado tiveram chance 5,6 vezes maior de ser portadores de neoplasia avançada, quando comparados com aqueles com CEA normal. A sobrevida foi influenciada pelo performance status (p = 0,001, extensão anatômica (p = 0,006, concentração aumentada do CEA (p = 0,043, mais que dois marcadores aumentados (p < 0,001 e tipo de tratamento (p < 0,001. O valor prognóstico da extensão da neoplasia atingiu o limite da significância (p = 0,052; entretanto, a presença de mais do que dois marcadores aumentados e a modalidade terapêutica tiveram valor prognóstico independente (respectivamente, p = 0,035 e p = 0,005. Conclusões: Nenhum dos marcadores tumorais séricos avaliados apresenta utilidade clínica no manejo dos pacientes com CNPCP.

  12. Comportamento sedentário e consumo de alimentos ultraprocessados entre adolescentes brasileiros: Pesquisa Nacional de Saúde do Escolar (PeNSE, 2015

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    Caroline dos Santos Costa

    2018-03-01

    Full Text Available Estudo transversal com o objetivo de investigar a associação entre comportamento sedentário e consumo de alimentos ultraprocessados (AUP em adolescentes brasileiros. Foram utilizados dados da Pesquisa Nacional de Saúde do Escolar (PeNSE realizada em 2015. O consumo diário de pelo menos um grupo de AUP representou o desfecho, e a exposição principal foi o tempo diário de comportamento sedentário (horas em atividades sentado, excluído o tempo dispendido na escola. Foram calculadas prevalências, razões de prevalências e intervalos de 95% de confiança (IC95%. As análises foram ajustadas para sexo, idade, cor da pele, escolaridade materna, índice de bens, região geográfica e dependência administrativa da escola. Cerca de 40% dos escolares reportaram consumo diário de pelo menos um grupo de AUP (39,7%; IC95%: 39,2-40,3 e 68,1% (IC95%: 67,7-68,7 referiram > 2 horas/dia de comportamento sedentário. Entre os escolares com comportamento sedentário > 2 horas/dia, a prevalência de consumo diário de AUP foi de 42,8% (IC95%: 42,1-43,6%, maior do que entre os sem comportamento sedentário (29,8%; IC95%: 29,0-30,5%. Quanto maior o tempo de comportamento sedentário, maior a prevalência de consumo de AUP (valor de p para tendência linear < 0,001. Estratégias que promovam a alimentação saudável e a diminuição de comportamentos sedentários, bem como regulamentações da publicidade de AUP, tornam-se necessárias a fim de evitar que estilos de vida não saudáveis perdurem à idade adulta.

  13. Vivência de violência entre escolares brasileiros: resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE Violence exposures by school children in Brazil: results from the National Adolescent School-based Health Survey (PeNSE

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    Deborah Carvalho Malta

    2010-10-01

    Full Text Available Este artigo apresenta os principais resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE. Aplicou-se questionário em uma amostra de conglomerados de 60.973 estudantes do 9º ano do ensino fundamental de escolas públicas e privadas das capitais dos estados brasileiros e do Distrito Federal, entre março e junho de 2009. Analisam-se prevalências e intervalos de confiança de 95% (IC95% das situações de violência envolvendo adolescentes. Foram identificadas as seguintes situações: insegurança no trajeto casa-escola (6,4%; IC95%: 6,1%-6,8% e na escola (5,5%; IC95%: 5,2%-5,8%; envolvimento em brigas com agressão física (12,9%; IC95%: 12,4%-13,4%, com arma branca (6,1%; IC95%: 5,7%-6,4% ou arma de fogo (4,0%; IC95%: 3,7%-4,3%; agressão física por familiar (9,5%; IC95%: 9,1%-9,9%. As situações de violência foram mais prevalentes entre estudantes do sexo masculino. Houve grande variação segundo as cidades estudadas. Os adolescentes estão expostos a diferentes manifestações de violência nas instituições que supostamente deveriam garantir sua proteção e desenvolvimento saudável e seguro - a escola e o lar. Esses resultados visam apoiar medidas de promoção à saúde e prevenção desses fatores de risco.This article presents the main results of the National Adolescent School-based Health Survey (PeNSE. A questionnaire was applied to a sample of 60,973 students of the 9th year of Junior high school in public and private schools of the Brazilian state capitals and the Federal District, between March and June 2009. The prevalence and confidence interval of 95% (CI 95% of the violence situations involving adolescents were analyzed. The following situations were identified: lack of safety on the way home-school (6.4%; CI95%: 6.1%-6.8% and at school (5.5%; CI95%: 5.2%-5.8%; involving fights with physical aggression (12.9%; CI95%: 12.4%-13.4%, with knife (6.1%; CI95%: 5.7%-6.4% or fire arm (4.0%; CI95%: 3.7%-4.3%; physical

  14. Serum and Urinary Cytokeratin 19 and Bladder Tumor Antigen in ...

    African Journals Online (AJOL)

    Conclusion Urinary CYFRA 21-1 and BTA stat are valuable non-invasive urinary markers for the detection of bladder cancer with a high sensitivity compared to urine cytology. Key Words cytokeratin, complement H, BTA, CYFRA 21-1, BTA stat. Résumé Cytokeratin 19 Sérique et Urinaire et Antigen Tumoral Vésical dans le ...

  15. Changes of serum endocrine hormone levels in patients with cancerrelated fatigue and their correlation with anti-tumor immune response and tumor load

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    Lu Yang

    2017-08-01

    Full Text Available Objective: To study the changes of serum endocrine hormone levels in patients with cancerrelated fatigue (CRF and their correlation with anti-tumor immune response and tumor load. Methods: A total of 137 patients who were diagnosed with primary lung cancer in West China Hospital, Sichuan University between June 2014 and November 2016 were selected and then divided into CRF group and control group according to their self-reported symptoms, serum was collected to determine the levels of endocrine hormones and tumor markers, and peripheral blood was collected to detect the levels of immune cells. Results: Serum ACTH and TSH levels of CRF group were significantly higher than those of control group while Cor, FT3 and FT4 levels were significantly lower than those of control group; peripheral blood CD11b+ CD15 - CD33+ CD14+ M-MDSC, CD11b+ CD15-CD33+ CD14- G-MDSC, CD4+ CD25+ CD127lowTreg and CD19+ CD5+ CD1d+ Breg levels as well as serum CEA, Cyfra21-1, SCC-Ag, HE4, GDF- 15 and PCNA levels of CRF group were significantly higher than those of control group, positively correlated with serum ACTH and TSH levels, and negatively correlated with Cor, FT3 and FT4 levels. Conclusion: The changes of thyroid hormone and adrenal cortical hormone levels in patients with cancer-related fatigue are closely related to the inhibited antitumor immune response and increased tumor load.

  16. Condição socioeconômica, frequência de escovação dentária e comportamentos em saúde em adolescentes brasileiros: uma análise a partir da Pesquisa Nacional de Saúde do Escolar (PeNSE Socioeconomic status, toothbrushing frequency, and health-related behaviors in adolescents: an analysis using the PeNSE database

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    Mario Vianna Vettore

    2012-01-01

    Full Text Available Investigou-se a associação entre comportamentos relacionados à saúde bucal e geral e condição socioeconômica, e a relação entre comportamentos relacionados à saúde e escovação dentária em adolescentes. A base de dados utilizada foi da Pesquisa Nacional de Saúde do Escolar (PeNSE, um estudo transversal de base populacional realizado em 2009 com alunos das 27 capitais brasileiras. Informações sociodemográficas e comportamentos relacionados à saúde foram coletados. O estudo envolveu 49.189 adolescentes (47,5% do sexo masculino, sendo a maioria com 14 anos de idade e de escola pública. A frequência de escovação dentária assim como outros comportamentos relacionados à saúde se associou com a condição socioeconômica de maneira distinta entre sexos. Foram observadas associações entre hábitos relacionados à saúde e a frequência de escovação dentária em ambos os sexos, porém com variações conforme a condição socioeconômica. O planejamento de intervenções para promoção da saúde de adolescentes deve levar em conta suas características individuais, o contexto familiar e social.This study investigated the association between oral and general health-related behaviors and socioeconomic status, and the relationship between health-related behaviors and toothbrushing among adolescents. The database used here was the National School-Based Health Survey (PeNSE, a cross-sectional population-based study in 2009 with students from 27 Brazilian State capitals. Socio-demographic and health-related behavior data were collected. The survey included 49,189 adolescents (47.5% males, the majority of whom were 14 years of age and enrolled in public schools. The associations between toothbrushing frequency and other health-related behaviors and socioeconomic status varied between boys and girls. Associations were observed between health-related habits and toothbrushing frequency in both sexes, but with variations according to

  17. Application of serum SELDI proteomic patterns in diagnosis of lung cancer

    International Nuclear Information System (INIS)

    Yang, Shuan-ying; Xiao, Xue-yuan; Zhang, Wang-gang; Zhang, Li-juan; Zhang, Wei; Zhou, Bin; Chen, Guoan; He, Da-cheng

    2005-01-01

    Currently, no satisfactory biomarkers are available to screen for lung cancer. Surface-Enhanced Laser Desorption/ionization Time-of- Flight Mass Spectrometry ProteinChip system (SELDI-TOF-MS) is one of the currently used techniques to identify biomarkers for cancers. The aim of this study is to explore the application of serum SELDI proteomic patterns to distinguish lung cancer patients from healthy individuals. A total of 208 serum samples, including 158 lung cancer patients and 50 healthy individuals, were randomly divided into a training set (including 11 sera from patients with stages I/II lung cancer, 63 from patients with stages III/IV lung cancer and 20 from healthy controls) and a blinded test set (including 43 sera from patients with stages I/II lung cancer, 41 from patients with stages III/IV lung cancer and 30 from healthy controls). All samples were analyzed by SELDI technology. The spectra were generated on weak cation exchange (WCX2) chips, and protein peaks clustering and classification analyses were made using Ciphergen Biomarker Wizard and Biomarker Pattern software, respectively. We additionally determined Cyfra21-1 and NSE in the 208 serum samples included in this study using an electrochemiluminescent immunoassay. Five protein peaks at 11493, 6429, 8245, 5335 and 2538 Da were automatically chosen as a biomarker pattern in the training set. When the SELDI marker pattern was tested with the blinded test set, it yielded a sensitivity of 86.9%, a specificity of 80.0% and a positive predictive value of 92.4%. The sensitivities provided by Cyfra21-1 and NSE used individually or in combination were significantly lower than that of the SELDI marker pattern (P < 0.005 or 0.05, respectively). Based on the results of the test set, we found that the SELDI marker pattern showed a sensitivity of 91.4% in the detection of non-small cell lung cancers (NSCLC), which was significantly higher than that in the detection of small cell lung cancers (P < 0.05); The

  18. Application of serum SELDI proteomic patterns in diagnosis of lung cancer

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    Zhou Bin

    2005-07-01

    Full Text Available Abstract Background Currently, no satisfactory biomarkers are available to screen for lung cancer. Surface-Enhanced Laser Desorption/ionization Time-of- Flight Mass Spectrometry ProteinChip system (SELDI-TOF-MS is one of the currently used techniques to identify biomarkers for cancers. The aim of this study is to explore the application of serum SELDI proteomic patterns to distinguish lung cancer patients from healthy individuals. Methods A total of 208 serum samples, including 158 lung cancer patients and 50 healthy individuals, were randomly divided into a training set (including 11 sera from patients with stages I/II lung cancer, 63 from patients with stages III/IV lung cancer and 20 from healthy controls and a blinded test set (including 43 sera from patients with stages I/II lung cancer, 41 from patients with stages III/IV lung cancer and 30 from healthy controls. All samples were analyzed by SELDI technology. The spectra were generated on weak cation exchange (WCX2 chips, and protein peaks clustering and classification analyses were made using Ciphergen Biomarker Wizard and Biomarker Pattern software, respectively. We additionally determined Cyfra21-1 and NSE in the 208 serum samples included in this study using an electrochemiluminescent immunoassay. Results Five protein peaks at 11493, 6429, 8245, 5335 and 2538 Da were automatically chosen as a biomarker pattern in the training set. When the SELDI marker pattern was tested with the blinded test set, it yielded a sensitivity of 86.9%, a specificity of 80.0% and a positive predictive value of 92.4%. The sensitivities provided by Cyfra21-1 and NSE used individually or in combination were significantly lower than that of the SELDI marker pattern (P P Conclusion These results suggest that serum SELDI protein profiling can distinguish lung cancer patients, especially NSCLC patients, from normal subjects with relatively high sensitivity and specificity, and the SELDI-TOF-MS is a potential tool

  19. Clinical Evaluation of Tumor Markers for Diagnosis in Patients with Non-small Cell Lung Cancer in China.

    Science.gov (United States)

    Ma, Li; Xie, Xiao-Wei; Wang, Hai-Yan; Ma, Ling-Yun; Wen, Zhong-Guang

    2015-01-01

    To evaluate the value of combined detection of serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1), and carbohydrateantigen 125 (CA125) for the clinical diagnosis of non- small cell lung cancer (NSCLC). Serum CEA, CYFRA21-1 and CA125 were assessed in 140 patients with NSCLC, 90 patients with benign lung disease and 90 normal control subjects, and differences of expression were compared in each group, and joint effects of these tumor markers in the diagnosis of NSCLC were analyzed. Serum CEA, CYFRA21-1 and CA125 in patients with NSCLC were significantly higher than those with benign lung disease and normal controls (PCEA, CYFRA21-1 and CA125 were 49.45%, 59.67%, and 44.87% respectively. As expected, combinations of these tumor markers improved their sensitivity for NSCLC. The combined detection of CEA+CYFRA21-1 was the most cost-effective combination which had higher sensitivity and specificity in NSCLC. Elevation of serum CEA and CYFRA21-1 was significantly associated with pathological types (PCEA, CYFRA21-1 and CA125 was significantly associated with TNM staging (PCEA, CYFRA21-1 and CA125 is of diagnostic value in the diagnosis of lung cancer, and a joint detection of these three tumor markers, could greatly improve the sensitivity of diagnosis on NSCLC. Combined detection of CEA+CYFRA21-1 proved to be the most economic and practical strategy in diagnosis of NSCLC, which can be used to screen the high-risk group.

  20. A sensitive label-free electrochemical immunosensor for detection of cytokeratin 19 fragment antigen 21-1 based on 3D graphene with gold nanopaticle modified electrode.

    Science.gov (United States)

    Zeng, Yan; Bao, Jing; Zhao, Yanan; Huo, Danqun; Chen, Mei; Yang, Mei; Fa, Huanbao; Hou, Changjun

    2018-02-01

    Previous studies have confirmed that cytokeratin 19 fragment antigen 21-1 (CYFRA 21-1) serves as a powerful biomarker in non-small cell lung cancer (NSCLC). Herein, we report for the first time a label-free electrochemical immunosensor for sensitive and selective detection of tumor marker CYFRA21-1. In this work, three-dimensional graphene @ gold nanoparticles (3D-G@Au) nanocomposite was modified on the glassy carbon electrode (GCE) surface to enhance the conductivity of immunosensor. The anti-CYFRA21-1 captured and fixed on the modified GCE through the cross-linking of chitosan (CS), glutaraldehyde (GA) and anti-CYFRA21-1. The differential pulse voltammetry (DPV) peak current change due to the specific interaction between anti-CYFRA21-1 and CYFRA21-1 on the modified electrode surface was utilized to detect CYFRA21-1. Under optimized conditions, the proposed electrochemical immunosensor was employed to detect CYFRA21-1 and exhibited a wide linear range of 0.25-800ngmL -1 and low detection limit of 100pgmL -1 (S/N = 3). Moreover, the recovery rates of serum samples were in the range from 95.2% to 108.7% and the developed immunosensor also shows a good correlation (less than 6.6%) with enzyme-linked immunosorbent assay (ELISA) in the detection of clinical serum samples. Therefore, it is expected that the proposed immunosensor based on a 3D-G@Au has great potential in clinical medical diagnosis of CYFRA21-1. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. Clinical significance of determination of 3 tumor markers in bronchoalveolar lavage fluid

    International Nuclear Information System (INIS)

    Chen Rui; Hu Huacheng; Hu Yunzhu

    2003-01-01

    Objective: To investigate the value of 3 tumor markers in bronchoalveolar lavage fluid (BALF) for diagnosis and evaluation of disease extent in patients with lung cancer. Methods: The level of CEA, CYFRA21-1 and NSE in BALF was measured in 92 patients with lung cancer and 40 patients with benign lung diseases by using chemoluminescence, RIA and ELISA methods respectively. Results: The level of all 3 tumor markers measured in BALF was much higher in lung cancer group than that in benign lung disease group (P<0.01 or P<0.05), and it was higher in patients with advanced disease (stage III and IV) than that in stage I and II. These tumor markers increased in different degrees among the patients in various pathological classifications. It was also found the level of these tumor markers was higher and more sensitive in BALF than that in serum. Conclusion: The measurement of the tumor markers in BALF has more significant value than the measurement in serum, which contribute to the early diagnosis, pathological classification and prognosis evaluation of lung cancer

  2. Effect of cryoablation sequential chemotherapy on patients with advanced non-small cell lung cancer

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    Shu-Hui Yao

    2016-03-01

    Full Text Available Objective: To evaluate the effect of cryoablation sequential chemotherapy on patients with advanced non-small cell lung cancer. Methods: A total of 39 cases with advanced non-small cell lung cancer who received cryoablation sequential chemotherapy and 39 cases with advanced non-small cell lung cancer who received chemotherapy alone were selected and enrolled in sequential group and control group, disease progression and survival of two groups were followed up, and contents of tumor markers and angiogenesis molecules in serum as well as contents of T-lymphocyte subsets in peripheral blood were detected. Results: Progressionfree survival and median overall survival (mOS of sequential group were longer than those of control group, and cumulative cases of tumor progression at various points in time were significantly less than those of control group (P<0.05; 1 month after treatment, serum tumor markers CEA, CYFRA21-1 and NSE contents, serum angiogenesis molecules PCDGF, VEGF and HDGF contents as well as CD3+CD4-CD8+CD28-T cell content in peripheral blood of sequential group were significantly lower than those of control group (P<0.05, and contents of CD3+CD4+CD8-T cell and CD3+CD4-CD8+CD28+T cell in peripheral blood were higher than those of control group (P<0.05. Conclusions: Cryoablation sequential chemotherapy can improve the prognosis of patients with advanced non-small cell lung cancer, delay disease progression, prolong survival time, inhibit angiogenesis and improve immune function.

  3. A new characterization of L2 (p) by NSE

    Indian Academy of Sciences (India)

    In this paper we give a new characterization of simple group 2() with a prime by both its order and (2()), the set of numbers of elements of 2() with the same order. Author Affiliations. Qinhui Jiang1 Changguo Shao1. School of Mathematical Sciences, University of Jinan, 250022 Shandong, China. Dates.

  4. A new characterization of L2(p) by NSE

    Indian Academy of Sciences (India)

    In this paper we give a new characterization of simple group L2(p) with ... Introduction. Throughout this paper, all groups are finite and G denotes a group. .... [5] Mazurov V D and Khukhro E I, Unsolved Problems in Group Theory, the Kourovka.

  5. Characterizations of sugar ball in solution by SANS and NSE

    International Nuclear Information System (INIS)

    Funayama, Katsuya; Imae, Toyoko; Aoi, Keigo; Tsutsumiuchi, Kaname; Okada, Masahiko; Seto, Hideki; Nagao, Michihiro

    2001-01-01

    Overall static and dynamic characterizations of the dendrimer are expected to be modified through changing the internal and terminal chemical structures of the dendrimer. In the present study, the aqueous solutions of the fifth generation glycopeptide-type sugar ball at 1 and 10 wt% dendrimer concentrations, which show different small-angle neutron scattering profiles, were measured by neutron spin echo. The diffusion behaviors were found to depend on dendrimer concentration, as well as for the solutions of fifth generation poly (amido amine) dendrimer with hydroxyl terminals. A slow relaxation mode was obtained at high concentration but the fast and slow modes were at low concentration. It was concluded that the slow mode is translational diffusion of dendrimer and the fast one due to segment motion in dendrimer. Since the fast mode was found for both dendrimer solutions, that will be specific dynamics originated by amido-amine unit in dendrimer, which is common in both dendrimers. (author)

  6. Diagnostic value of tumor markers for lung adenocarcinoma-associated malignant pleural effusion: a validation study and meta-analysis.

    Science.gov (United States)

    Feng, Mei; Zhu, Jing; Liang, Liqun; Zeng, Ni; Wu, Yanqiu; Wan, Chun; Shen, Yongchun; Wen, Fuqiang

    2017-04-01

    Pleural effusion is one of the most common complications of lung adenocarcinoma and is diagnostically challenging. This study aimed to investigate the diagnostic performance of carcinoembryonic antigen (CEA), cytokeratin fragment (CYFRA) 21-1, and cancer antigen (CA) 19-9 for lung adenocarcinoma-associated malignant pleural effusion (MPE) through a validation study and meta-analysis. Pleural effusion samples were collected from 81 lung adenocarcinoma-associated MPEs and 96 benign pleural effusions. CEA, CYFRA 21-1, and CA19-9 were measured by electrochemiluminescence immunoassay. The capacity of tumor markers was assessed with receiver operating characteristic curve analyses and the area under the curve (AUC) was calculated. Standard methods for meta-analysis of diagnostic studies were used to summarize the diagnostic performance of CEA, CYFRA 21-1, and CA19-9 for lung adenocarcinoma-associated MPE. The pleural levels of CEA, CYFRA 21-1, and CA19-9 were significantly increased in lung adenocarcinoma-associated MPE compared to benign pleural effusion. The cut-off points for CEA, CYFRA 21-1, and CA19-9 were optimally set at 4.55 ng/ml, 43.10 μg/ml, and 12.89 U/ml, and corresponding AUCs were 0.93, 0.85, and 0.81, respectively. The combination of CEA, CYFRA 21-1, and CA19-9 increased the sensitivity to 95.06%, with an AUC of 0.95. Eight studies were included in this meta-analysis. CEA showed the best diagnostic performance with pooled sensitivity, specificity, positive/negative likelihood ratio, and diagnostic odds ratio of 0.75, 0.96, 16.01, 0.23, and 81.49, respectively. The AUC was 0.93. CEA, CYFRA 21-1, and CA19-9 play a role in the diagnosis of lung adenocarcinoma-associated MPE. The combination of these tumor markers increases the diagnostic accuracy.

  7. The relationship between Glasgow Prognostic Score and serum tumor markers in patients with advanced non-small cell lung cancer.

    Science.gov (United States)

    Jiang, Ai-Gui; Chen, Hong-Lin; Lu, Hui-Yu

    2015-05-10

    Glasgow Prognostic Score (GPS) has been reported as a powerful prognostic tool for patients with advanced non-small cell lung cancer (NSCLC). The aim of this study was to assess the relationship between GPS and prognosis related tumor markers in patients with advanced NSCLC. We included 138 advanced NSCLC patients and twenty healthy controls in the study. GPS was calculated by combined serum C-reactive protein (CRP) and albumin. Three serum tumor markers, which included cytokeratin 19 fragment antigen 21-1 (CYFRA21-1), carcinoembryonic antigen (CEA) and tissue polypeptide specific antigen (TPS), were detected by enzyme-linked immunosorbent assay (ELISA). GPS and tumor markers were all assessed before chemotherapy. All patients received at least 2 courses of cisplatin-based chemotherapy. After that, 2 to 5 years follow-up was conducted. Median levels of CYFRA21-1 were 1.5 ng/ml (0.1-3.1 ng/ml) in healthy controls, and 4.6 ng/ml (0.7-35.2 ng/ml) in GPS 0 advanced NSCLC, 11.2 ng/ml (0.4-89.2) ng/ml in GPS 1 advanced NSCLC, and 15.7 ng/ml (2.9-134.6 ng/ml) in GPS 2 advanced NSCLC, respectively. Median levels of CYFRA21-1 were higher in NSCLC patients than in healthy controls, and CYFRA21-1 increased gradually according to GPS category in NSCLC patients (PGPS, CEA and GPS, TPS and GPS. The Spearman's rank correlation coefficient were 0.67 (P GPS was an independent prognostic factor for advanced NSCLC. CYFRA21-1(>3.3 ng/ml) and TPS (>80 U/l) were related with the prognosis of advanced NSCLC by univariate analyses, but multivariate analyses showed CYFRA21-1, TPS and CEA were not the independent prognostic factors for advanced NSCLC. Our results showed GPS were positive correlated with CYFRA21-1, CEA and TPS in patients with advanced NSCLC. However, GPS was more efficient in predicting prognosis of advanced NSCLC than these three single prognosis related tumor markers.

  8. Study on the clinical value of combined determination of six tumor marker for diagnosis of pulmonary carcinoma

    International Nuclear Information System (INIS)

    Chen Zhong; Liu Yun; Liu Li; Lu Xiaopeng; Zhang Jun; Li Jiangang; Zhu Zili

    2006-01-01

    Objective: To study the clinical applicability of single/combined determination of six tumor markers (CEA, CA125, CA50, CA19-9, CA153, CYFRA21-1) for diagnosis of pulmonary carcinoma. Methods: Serum contents of these six tumor markers were determined with RIA in 130 patients with pulmonary carcinoma, 40 patients with various benign pulmonary disorders, and 45 controls. Results: (1) Only two false positive cases were found in the 40 patients with benign pulmonary disorders (CA125, n=1, CA153 n=1). (2) Positive rate of single marker in patients with pulmonary cancer: CYFRA21-1 (79.23%)>CA153 (61.53%) > CA125(58.46%)> CA19-9(57.69%) > CEA(46.92%) > CA50(43.84%). (3) Combined determination of CYFRA21-1 with any one of the other 5 markers would increase the positive rate to 86.15%-89.23%. (4) Mean value of any marker in the malignant cases were over 4 folds of that in the benign cases-high diagnostic credibility. (5) Histology of the malignancy (squamous cell carcinoma or adenocarcinoma) made no difference on the positive rate of the markers with the exception of a slight higher positive rate of CA19-9 in adenocarcinomas. Conclusion: The authors believe that combined determination of CYFRA21-1 with CA153 would be the first choice for diagnosis of pulmonary carcinoma, followed by CYFRA21-1 + CA125 or CA19-9. (authors)

  9. Pleural effusion biomarkers and computed tomography findings in diagnosing malignant pleural mesothelioma: A retrospective study in a single center

    Science.gov (United States)

    Kataoka, Yuki; Ikegaki, Shunkichi; Saito, Emiko; Matsumoto, Hirotaka; Kaku, Sawako; Shimada, Masatoshi; Hirabayashi, Masataka

    2017-01-01

    In this study, we aimed to examine the clinical value of the pleural effusion (PE) biomarkers, soluble mesothelin-related peptide (SMRP), cytokeratin 19 fragment (CYFRA 21–1) and carcinoembryonic antigen (CEA), and the utility of combining chest computed tomography (CT) findings with these biomarkers, in diagnosing malignant pleural mesothelioma (MPM). We conducted a retrospective cohort study in a single center. Consecutive patients with undiagnosed pleural effusions who underwent PE analysis between September 2014 and August 2016 were reviewed. This study included 240 patients (32 with MPM and 208 non-MPM). SMRP and the CYFRA 21-1/CEA ratio had a sensitivity and specificity for diagnosing MPM of 56.3% and 86.5%, and 87.5% and 74.0%, respectively. Using receiver operating characteristics (ROC) curve analysis of the ability of these markers to distinguish MPM from all other PE causes, the area under the ROC curve (AUC) for SMRP and the CYFRA 21-1/CEA ratio was 0.804 and 0.874, respectively. The sensitivity and specificity of SMRP combined with the CYFRA 21-1/CEA ratio were 93.8% and 64.9%, respectively. The sensitivity of the combination of SMRP, the CYFRA 21-1/CEA ratio, and the presence of Leung’s criteria (a chest CT finding that is suggestive of malignant pleural disease) was 93.8%. In conclusion, the combined PE biomarkers had a high sensitivity for diagnosing MPM, although the addition of chest CT findings did not improve the sensitivity of SMRP combined with the CYFRA 21-1/CEA ratio. Combination of these biomarkers helped to rule out MPM effectively among patients at high risk of suffering MPM and would be valuable especially for old frail patients who have difficulty in undergoing invasive procedures such as thoracoscopy. PMID:28968445

  10. Establishment and Characterization of a Novel Chinese Human Lung Adenocarcinoma Cell Line CPA-Yang2 in Immunodeficient Mice

    Directory of Open Access Journals (Sweden)

    Shunfang YANG

    2009-10-01

    Full Text Available Background and objective The recurrence, metastasis and multidrug resistance (MDR in lung cancer are the tough problems worldwide. This study was to establish a novel chinese lung adenocarcinoma cell line with high metastasis potential for exploring the mechanism of reccurrence, development and MDR in lung cancer. Methods The cell came from the abdominal dropsy of a fifty-six years old female patient with lung adenocarcinoma and the tumor markers CA125, CYFRA21-1, CEA, NSE were detected to be higher secretion by radioimmunoassay in the abdominal dropsy. Tumorigenicity of immunodeficient mice was confirmed in 8th passage. The cell growth curve was mapped. Analysis of chromosome karyotype was tested. The gene expression was measured by real-time quantitative PCR. Results The tumorigenesis rate started at 8th passage in 3/10 immunodeficient mice via subcutaneously and the fully tumorigenicity was at 11th passage as well as later passages. Under the microscope, the cell showed oval-shap and adherence. The chromosome karyotype analysis of the cells was sub-triploid. Approximately 1×106 and 1.5×106 cancerous cells were injected into left cardiac ventricle and tail vein of immunodeficient mice respectively. The results showed multiorgan metastasis in the mice after three-four weeks, including mandible, scapula, humerus, vertebral column, femur, rib and brain, liver, adrenal gland, pulmonary in the mice after inoculation. The bone metastasis rate was 100% in the tumor bearing mice by bone scintigraphy and pathology. Quantitative real-time PCR was used to examined and compared with SPC-A-1 lung adenocarcinoma, ESM1, VEGF-C, IL-6, IL-8, AR genes were overexpressed. The novel cell was named CPA-Yang2. Conclusion The characteristics of novel strain CPA-Yang2 is a highly metastasis cell line of Chinese lung adenocarcinoma. It has stable traits, highly metastasis ability and maybe is a MDR lung cancerous cell line. Of course, it’s a good experimental

  11. Diagnostic utility of serum and pleural fluid carcinoembryonic antigen, and cytokeratin 19 fragments in patients with effusion from nonsmall cell lung cancer

    Directory of Open Access Journals (Sweden)

    Sushil Kumar Sharma

    2015-01-01

    Full Text Available Aims: To assess the diagnostic value of CEA and CYFRA 21-1 (cytokeratin 19 fragments in serum and pleural fluid in non small cell lung cancer with malignant pleural effusion (MPE. Settings and Design: Two subsets of patients were recruited with lymphocytic exudative effusion, one subset constituted diagnosed patients of NSCLC with malignant pleural effusion and the other subset of constituted with Tubercular pleural effusion. Methods and Material : CYFRA 21-1 and CEA levels were measured using Electrochemilumiscence Immunoassay (ECLIA. The test principle used the Sandwich method. For both the tests, results are determined via a calibration curve which is instrument specifically generated by 2 - point calibration and a master curve provided via reagent barcode. Statistical Analysis Used: All data are expressed as means ± SD and percentage. All the parametric variables were analysed by student-t test where as non parametric variables were compared by Mann-Whitney U-test Statistical significance was accepted for P values < 0.05. Software used were SPSS 11.5, and MS excel 2007. In order to compare the performance of the tumor markers, receiver operating characteristic (ROC curves were constructed and compared with area under the curve (AUC. The threshold for each marker was selected based on the best diagnostic efficacy having achieved equilibrium between sensitivity and specificity. Results: In cases serum CYFRA21-1 levels had mean value of 34.1 ± 29.9 with a range of 1.6-128.3 where as in controls serum CYFRA21-1 levels had mean value of 1.9 ± 1.0 with a range of 0.5-4.7. In cases serum CEA levels had mean value of 24.9 ± 47.3 with a range of 1.0, 267.9 where as in controls serum CEA levels had mean value of 1.9 ± 1.4 with a range of 0.2-6.8. The difference in the means of serum CYFRA 21-l (P = 0.000 and CEA (P = 0.046 were statistically significant. In cases pleural fluid CYFRA21-1 levels had mean value of 160.1 ± 177.1 with a range of 5

  12. Long term follow up results of serum squamous cell carcinoma antigen level in uterine cervix cancer treated by radiotherapy

    International Nuclear Information System (INIS)

    Yun, Hyong Geun

    2003-01-01

    To evaluate the long term significance of the squamous cell carcinoma (SCC) antigen (Ag) as a tumor marker in uterine cervix carcinoma. The SCC antigen levels of pre-radiotherapy and serial post-radiotherapy serum were analyzed in 48 patients who received radiotherapy with histologically proven primary SCC of the uterine cervix. Pre-radiotherapy SCC Ag level was high (≥2 ng/ml) at 79.2%. After the treatment, the SCC Ag level was significantly decreased. The SCC Ag level measured at about 3 months after radiotherapy was high at 23.0%. In further follow up measurements, a rise of the SCC Ag to a high level was well associated with clinical relapse. The specificity of the elevated SCC Ag level in association with recurrent or persistent disease was 100%, and the sensitivity was 85.7%. In 3 of 4 lung metastasis cases, lung lesions were detected in chest PA before elevation of the SCC Ag level. The median lead time of the high SCC Ag level to clinical recurrence was 4 months. SCC Ag was a good tumor marker for monitoring treatment effect in patients with increased pre-treatment levels except in case of early lung metastasis. Elevation of the SCC Ag level after radiotherapy accurately predicted the treatment failure with lead time of 4 months. But, in early lung metastasis cases, the SCC level may be normal temporarily. Thus, chest PA should be checked to evaluate the presence of lung metastasis

  13. Prognostic value of pretreatment serum carcinoembryonic antigen and squamous cell carcinoma antigen levels for patients with stage I-III non-small cell lung cancer treated with radiation therapy alone

    International Nuclear Information System (INIS)

    Saito, Yoshihiro; Mitsuhashi, Norio; Hayakawa, Kazushige

    1998-01-01

    Serum carcinoembryonic antigen (CEA) and serum squamous cell carcinoma antigen (SCC Ag) levels have been reported to be useful as prognostic factors, indicators of clinical response, and predictors for recurrence in patients with lung cancer treated by surgery or chemotherapy. We investigated whether pretreatment serum CEA and SCC Ag levels were useful as independent prognostic factors in patients with stage I to III non-small cell lung cancer who were treated with radiation therapy alone. The serum CEA and SCC Ag levels were measured in 158 and 47 patients, respectively, before radiation therapy. Serum CEA and SCC Ag levels were measured by sandwich radioimmunoassay using the CEA-RIA (radioimmunoassay) kit and the SCC-RIA kit. Serum CEA and SCC Ag levels were above reference values in 19% and 30% of the patients, respectively. The 5-year survival rates were significantly better for patients with a negative SCC Ag result than for those with positive SCC Ag levels (p=0.0001), though no significant difference in survival rates was seen by CEA positivity (p=0.25). SCC Ag positivity (p=0.0006) and stage (p=0.04) were the important prognostic factors, as determined by multivariate analyses. Pretreatment serum SCC Ag level may be useful as an independent prognostic factor in patients with stage I to III non-small cell lung cancer who are treated with radiation therapy alone. (author)

  14. The relationship between glasgow prognostic score and serum tumor markers in patients with advanced non-small cell lung cancer

    International Nuclear Information System (INIS)

    Jiang, Ai-Gui; Chen, Hong-Lin; Lu, Hui-Yu

    2015-01-01

    Glasgow Prognostic Score (GPS) has been reported as a powerful prognostic tool for patients with advanced non–small cell lung cancer (NSCLC). The aim of this study was to assess the relationship between GPS and prognosis related tumor markers in patients with advanced NSCLC. We included 138 advanced NSCLC patients and twenty healthy controls in the study. GPS was calculated by combined serum C-reactive protein (CRP) and albumin. Three serum tumor markers, which included cytokeratin 19 fragment antigen 21-1 (CYFRA21–1), carcinoembryonic antigen (CEA) and tissue polypeptide specific antigen (TPS), were detected by enzyme-linked immunosorbent assay (ELISA). GPS and tumor markers were all assessed before chemotherapy. All patients received at least 2 courses of cisplatin-based chemotherapy. After that, 2 to 5 years follow-up was conducted. Median levels of CYFRA21–1 were 1.5 ng/ml (0.1–3.1 ng/ml) in healthy controls, and 4.6 ng/ml (0.7–35.2 ng/ml) in GPS 0 advanced NSCLC, 11.2 ng/ml (0.4–89.2) ng/ml in GPS 1 advanced NSCLC, and 15.7 ng/ml (2.9–134.6 ng/ml) in GPS 2 advanced NSCLC, respectively. Median levels of CYFRA21-1 were higher in NSCLC patients than in healthy controls, and CYFRA21-1 increased gradually according to GPS category in NSCLC patients (P < 0.05). Similar results were found for median levels of CEA and TPS in healthy controls and NSCLC patients (P < 0.05). In NSCLC patients, positive correlations were found between CYFRA21-1 and GPS, CEA and GPS, TPS and GPS. The Spearman’s rank correlation coefficient were 0.67 (P < 0.05), 0.61 (P < 0.05) and 0.55 (P < 0.05), respectively. Survival analyses showed GPS was an independent prognostic factor for advanced NSCLC. CYFRA21-1(>3.3 ng/ml) and TPS (>80 U/l) were related with the prognosis of advanced NSCLC by univariate analyses, but multivariate analyses showed CYFRA21-1, TPS and CEA were not the independent prognostic factors for advanced NSCLC. Our results showed GPS were positive correlated

  15. Measurement of Lung Cancer Tumor Markers in a Glass Wool Company Workers Exposed to Respirable Synthetic Vitreous Fiber and Dust

    Directory of Open Access Journals (Sweden)

    Shabnam Abtahi

    2018-01-01

    Full Text Available Background: Occupational exposures to respirable synthetic vitreous fiber (SVF and dust are associated with many lung diseases including lung cancer. Low-dose computed tomography is used for screening patients who are highly suspicious of having lung carcinoma. However, it seems not to be cost-effective. Serum biomarkers could be a useful tool for the surveillance of occupational exposure, by providing the possibility of diagnosing lung cancer in its early stages. Objective: To determine if serum carcinoembryonic antigen (CEA and cytokeratin fragment (CYFRA 21-1 levels in workers exposed more than normal population to respirable SVF and dust may be used as indicators of progression towards lung cancer. Methods: An analytic cross-sectional study, including 145 personnel of a glass wool company, along with 25 age-matched healthy individuals, was conducted to investigate the relationship between occupational exposure to respirable SVFs and dust and serum levels of two lung/pleura serum tumor markers, CEA and CYFRA 21-1, measured by ELISA. Results: Individuals exposed to higher than the recommended levels of respirable SVF had higher serum concentrations of CEA and CYFRA 21-1, compared to controls (p=0.008 and 0.040, respectively, as well as in comparison to those exposed to lower than recommended OSHA levels (p=0.046 and 0.033, respectively. Workers with >9 years work experience, had significantly (p=0.045 higher levels of serum CYFRA 21-1 than those with ≤9 years of experience. Conclusion: It seems that working for >9 years in sites with detectable levels of respirable SVF and dust would increase the levels of known lung cancer serum tumor markers. Transferring these workers to sites with respirable SVF concentrations lower than the limit of detection in the air is recommended.

  16. Clinical value of combined detection of tumor markers in effusion fluid for diagnosis of malignant pleural effusion and ascites

    International Nuclear Information System (INIS)

    Li Jiangang; Ji Zhigu; Cui Xuejun; Zhu Zili

    2010-01-01

    Objective: To study the clinical usefulness of combined detection of tumor markers in effusion fluid in patients with malignant pleural effusion or ascites. Methods: Combined detection of six tumor markers (CA125, CA50, CA15-3, CYFRA21-1, βHCG, HCG) in effusion fluid was performed in 92 patients with malignant pleural effusion and 78 patients with malignant ascites as well as 100 control benign specimens. These tumor markers were examined with CLIA, except CA50, which was examined with RIA. Exfoliative cytology was also examined in the malignant specimens. Results: The positive rate of these markers was highest with CA125, followed by CA50, CA15-3, CYFRA21-1, βHCG and HCG in order. βHCG and HCG, though with quite low positive rate, were still useful markers due to the almost zero false-positive rate, i.e. very high specificity. For combined determination of two markers, CA15 + CYFRA21-1 or CA125 + CA50 would result in the highest positive rate. For highly suspected but undetermined cases, the following criteria for malignancy would be helpful: (1) two or more positive among CA125, CA50, CA15-3, CYFRA21-1 (2) One of the four CAs positive + either βHCG or HCG (3) Both βHCG and HCG positive. Tumor markers positiveness would be supplementary to doubtful cytological studies. Conclusion: Combined detection of tumor markers in effusion fluid would be very helpful for diagnosis of malignancy. (authors)

  17. Dynamic observation on changes of serum tumor markers levels after implantation of 125I radioactive seeds as treatment for several malignancies

    International Nuclear Information System (INIS)

    Sun Qihe; Yang Jiali; Gao Mingzhong

    2005-01-01

    Objective: To study the dynamic changes of serum levels of several tumor markers after implantation of 125 I seeds as treatment for breast, prostate and lung malignancies. Methods: Serum CA15-3 (in 48 cases of breast cancer), PSA (in 59 cases of prostate cancer) and CYFRA21-1 (in 59 cases of lung cancer) levels were measured with RIA both before and after implantation of 125 I seeds as treatment. Furthermore, dynamic observation on the serum markers levels was carried out every 3 months in ten patients in each category. Results: After treatment, levels of these markers dropped significantly. Dynamic observation revealed that in the 10 cases of breast cancer, the levels of CA15-3 dropped continually. However, in the 10 cases of prostatic cancer, the disease got worse and the PSA levels kept increasing. In the lung cancer group, the CYFRA21-1 levels rose markedly and all patients expired before 9 months. Conclusion: Dynamic observation on changes of serum tumor markers (CA15-3, PSA, CYFRA21-1) levels after 125 I seed implantation treatment was of definite prognostic value. (authors)

  18. The use of other tobacco products among Brazilian school children (PeNSE 2012).

    Science.gov (United States)

    Hallal, Ana Luiza de Lima Curi; Figueiredo, Valeska Carvalho; Moura, Lenildo de; Prado, Rogério Ruscitto do; Malta, Deborah Carvalho

    2017-09-21

    The goal of this paper is to estimate the prevalence and to identify factors related to the use of other tobacco products among schoolchildren. A cross-sectional study was conducted with a representative sample of high school students enrolled in the 9th grade. A total of 109,104 students were interviewed, and 4.8% of them had used other tobacco products in the previous 30 days. The factors that increased the likelihood of using other tobacco products were: male gender, being administratively dependent on the school, having a job, living with mother and/or father, perception that the parents or guardians would not care if they smoked, having difficulties sleeping, not having close friends, having experienced domestic violence, skipping classes, having used tobacco and alcohol within the past 30 days, having experimented drugs, having smoking parents or guardians, having seen people smoking. The prevalence of using other tobacco products is high among Brazilian students, and is associated with higher socioeconomic conditions, presence of risk behavior, and living in an environment permissible to tobacco use.

  19. The causes of bullying: results from the National Survey of School Health (PeNSE

    Directory of Open Access Journals (Sweden)

    Wanderlei Abadio de Oliveira

    2015-04-01

    Full Text Available Objective: to identify the characteristics and reasons reported by Brazilian students for school bullying. Method: this cross-sectional study uses data from an epidemiological survey (National Survey of School Health conducted in 2012. A total of 109,104 9th grade students from private and public schools participated. Data were collected through a self-applied questionnaire and the analysis was performed using SPSS, version 20, Complex Samples Module. Results: the prevalence of bullying was 7.2%, most frequently affecting Afro-descendant or indigenous younger boys, whose mothers were characterized by low levels of education. In regard to the reasons/causes of bullying, 51.2% did not specify; the second highest frequency of victimization was related to body appearance (18.6%; followed by facial appearance (16.2%; race/color (6.8%; sexual orientation 2.9%; religion 2.5%; and region of origin 1.7%. The results are similar to those found in other sociocultural contexts. Conclusion: the problem belongs to the health field because it gathers aspects that determine the students' health-disease-care continuum.

  20. New software engineering paradigm based on complexity science an introduction to NSE

    CERN Document Server

    Xiong, Jay

    2011-01-01

    This book describes a revolution in software engineering - the Nonlinear Software Engineering paradigm, which complies with the essential principles of complexity science and can help double productivity, halve costs and reduce defects in software products.

  1. Måling af prokalcitonin kan muligvis begrænse antibiotikaforbruget ved pneumoni

    DEFF Research Database (Denmark)

    Berg, Peter; Lindhardt, Bjarne Ørskov

    2012-01-01

    with promising reductions in antibiotic use and treatment duration, but more research is needed in order to draw a final conclusion. Lack of objective diagnostic criteria, methodological challenges in research, a future focus on primary care, and the need for a cost-benefit analysis should be considered....

  2. Et værk, der ikke lader sig begrænse

    DEFF Research Database (Denmark)

    Hansen, Kamma Overgaard

    2017-01-01

    Det er ikke synd for naturen. Det er heller ikke synd for Aros. Men måske er det synd for kunsten. Katharina Grosses farveorgie 'The Garden' i Mindeparken har bragt sindene i kog og vendt op og ned på de politiske fløje og gjort det næsten umuligt at se værket som en æstetisk oplevelse frem...

  3. Evaluering af Tysklands 0-promillegrænse for nye motorførere

    DEFF Research Database (Denmark)

    Sørensen, Poul Lyk; Bernhoft, Inger Marie

    2012-01-01

    Den 1. august 2007 blev der indført totalforbud i Tyskland mod indtagelse af alkohol i forbindelse med bilkørsel for alle nyuddannede motorførere i den (normalt) toårige prøvetid og/eller alle unge under 21 år. Effekten af tiltaget er evalueret af Bundesanstalt für Strassenwesen (BASt).......Den 1. august 2007 blev der indført totalforbud i Tyskland mod indtagelse af alkohol i forbindelse med bilkørsel for alle nyuddannede motorførere i den (normalt) toårige prøvetid og/eller alle unge under 21 år. Effekten af tiltaget er evalueret af Bundesanstalt für Strassenwesen (BASt)....

  4. Måling af prokalcitonin kan muligvis begrænse antibiotikaforbruget ved pneumoni

    DEFF Research Database (Denmark)

    Berg, Peter; Lindhardt, Bjarne Ørskov

    2012-01-01

    Reduction of antimicrobial resistance in microorganisms is imperative. Pneumonia is important in this matter because of its high incidence, subjective diagnostic criteria, and variations in aetiology. Research has focused on the use of a procalcitonin-guided algorithm for antimicrobial stewardship...

  5. HISTORICAL AND IMPLIED VOLATILITY: AN INVESTIGATION INTO NSE NIFTY FUTURES AND OPTIONS

    OpenAIRE

    N R Parasuraman; P.Janaki Ramudu

    2011-01-01

    The broad objective of the paper is to have an understanding of the movement of volatility over a fair period in respect of the market portfolio. Also, it enables an understanding on how divergent the implied volatility has been from this estimate. It uses Volatility Cone, Volatility Smile and Volatility Surface as the parameters. The study takes different rolling periods percentiles of volatility. Hoadley Options Calculator is used for calculation and analysis purpose. The study empirically...

  6. Brain injury markers (S100B and NSE) in chronic cocaine dependents

    OpenAIRE

    Kessler, Felix Henrique Paim; Woody, George; Portela, Luis Valmor Cruz; Tort, Adriano Bretanha Lopes; De Boni, Raquel Brandini; Peuker, Ana Carolina Wolf Baldino; Genro, Vanessa Krebs; Diemen, Lisia von; Souza, Diogo Onofre Gomes de; Pechansky, Flavio

    2007-01-01

    Objetivo: Estudos têm demonstrado sinais de lesão cerebral causadas por diferentes mecanismos em usuários de cocaína. A enolase sérica neurônio-específica e a proteína S100B são consideradas marcadores bioquímicos específicos de lesão neuronal e glial. Este estudo objetivou comparar os níveis sangüíneos de S100B e enolase sérica neurônio-específica em usuários crônicos de cocaína e em voluntários que não usam cocaína ou outras drogas ilícitas. Método: Vinte sujeitos dependentes de cocaína, ma...

  7. Effects of Thoracic Paravertebral Block on Postoperative Analgesia and Serum Level of Tumor Marker in Lung Cancer Patients Undergoing Video-assisted Thoracoscopic Surgery

    Directory of Open Access Journals (Sweden)

    Jiheng CHEN

    2015-02-01

    Full Text Available Background and objective Perioperative management of pain associated with the prognosis of cancer patients. Optimization of perio-perative analgesia method, then reduce perioperative stress response, reduce opioiddosage, to reduce or even avoid systemic adverse reactions and elevated levels of tumor markers. Serum levels of tumor markers in patients with lung cancer are closely related to tumor growth. Clinical research reports on regional anesthesia effect on tumor markers for lung cancer are still very little in domesticliterature. The aim of this study is to evaluate the effects of thoracic paraverte-bral block on postoperative analgesia and serum level of tumor marker in lung cancer patients undergoing video-assisted thoracoscopic surgery. Methods Lung cancer patients undergoing video-assisted thoracoscopic surgery were randomly divided into 2 groups (n=20 in each group. The patients in group G were given only general anesthesia. The thoracic paravertebral blockade (PVB was performed before general anesthesia in patients of group GP. The effect of PVB was judged by testing area of block. Patient controlled intravenous analgesia (PCIA pump started before the end of surgery in 2 groups. Visual analogue scale (VAS score was recorded after extubation 2 h (T1, 24 h (T2 and 48 h (T3 after surgery and the times of PCIA and the volume of analgesic drugs used were recorded during 48 h after surgery. The serum levels of carcino-embryonic antigen (CEA, carbohydrate antigen 199 (CA199, carbohydrate antigen 125 (CA125, neuron-specific enolase (NSE, cytokeratin 19 fragment (CYFRA21-1 and squamous cell carcinoma (SCC in 40 lung cancer cases undergoing video-assisted thoracoscopic lobectomy were measured before operation and 24 h after operation. Results Forty American Society of Anesthesiologists (ASA physical status I or II patients, aged 20 yr-70 yr, body mass index (BMI 18 kg/m2-25 kg/m2, scheduled for elective video-assisted thoraeoscopic lobectomy

  8. Correlation Between Squamous Cell Carcinoma Antigen Level and the Clinicopathological Features of Early-Stage Cervical Squamous Cell Carcinoma and the Predictive Value of Squamous Cell Carcinoma Antigen Combined With Computed Tomography Scan for Lymph Node Metastasis.

    Science.gov (United States)

    Xu, Dianbo; Wang, Danbo; Wang, Shuo; Tian, Ye; Long, Zaiqiu; Ren, Xuemei

    2017-11-01

    The aim of this study was to analyze the relationship between serum squamous cell carcinoma antigen (SCC-Ag) and the clinicopathological features of cervical squamous cell carcinoma. The value of SCC-Ag and computed tomography (CT) for predicting lymph node metastasis (LNM) was evaluated. A total of 197 patients with International Federation of Gynecology and Obstetrics stages IB to IIA cervical squamous cell carcinoma who underwent radical surgery were enrolled in this study. The SCC-Ag was measured, and CT scans were used for the preoperative assessment of lymph node status. Increased preoperative SCC-Ag levels were associated with International Federation of Gynecology and Obstetrics stage (P = 0.001), tumor diameter of greater than 4 cm (P 4 cm (P = 0.001, OR = 4.019), and greater than one half stromal infiltration (P = 0.002, OR = 3.680) as independent factors affecting SCC-Ag greater than or equal to 2.35 ng/mL. In the analysis of LNM, SCC-Ag greater than or equal to 2.35 ng/mL (P < 0.001, OR = 4.825) was an independent factor for LNM. The area under the receiver operator characteristic curve (AUC) of SCC-Ag was 0.763 for all patients, and 0.805 and 0.530 for IB1 + IIA1 and IB2 + IIA2 patients, respectively; 2.35 ng/mL was the optimum cutoff for predicting LNM. The combination of CT and SCC-Ag showed a sensitivity and specificity of 82.9% and 66% in parallel tests, and 29.8% and 93.3% in serial tests, respectively. The increase of SCC-Ag level in the preoperative phase means that there may be a pathological risk factor for postoperative outcomes. The SCC-Ag (≥2.35 ng/mL) may be a useful marker for predicting LNM of cervical cancer, especially in stages IB1 and IIA1, and the combination of SCC-Ag and CT may help identify patients with LNM to provide them with the most appropriate therapeutic approach.

  9. Bullying in Brazilian schools: results from the National School-based Health Survey (PeNSE, 2009 Bullying nas escolas brasileiras: resultados da Pesquisa Nacional de Saúde do Escolar (PeNSE, 2009

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2010-10-01

    Full Text Available The aim of this study is to identify and describe the occurrence of bullying among students in the 9th year (8th grade from public and private schools from 26 Brazilian state capitals and the Federal District. It is a cross-sectional study involving 60,973 students and 1,453 public and private schools. Data analysis indicates that 5.4% (IC95%: 5.1%-5.7% of students reported having suffered bullying almost always or always in the last 30 days, 25.4% (IC95%: 24.8%-26.0% were rarely or sometimes the victim of bullying and 69.2% (IC95%: 68.5%-69.8% of students felt no humiliation or provocation at school. The capital with higher frequency of bullying was Belo Horizonte (6.9%; IC95%: 5,9%-7,9%, Minas Gerais, and the lowest was Palmas (3.5%; IC95%: 2.6%-4.5%, Tocantins. Boys reported more bullying (6,0%; IC95%: 5.5%-6.5% compared with girls (4,8%; IC95%: 4.4%-5.3%. There was no difference between public schools 5.5% (IC95%: 5.1%-5.8% and private (5.2% (IC95%: 4.6%-5.8%, except in Aracaju, Sergipe, that show more bullying in private schools. The findings indicate an urgent need for intersectoral action from educational policies and practices that enforce the reduction and prevention of the occurrence of bullying in schools in Brazil.O objetivo deste estudo é identificar e descrever a ocorrência do bullying, episódios de humilhação ou provocação perpetrados pelos colegas da escola, entre estudantes do 9º ano do ensino fundamental de escolas públicas e privadas das 26 capitais dos estados brasileiros e do Distrito Federal. Trata-se de estudo transversal feito com 60.973 escolares de 1.453 escolas públicas e privadas. A análise dos dados aponta que 5,4% (IC95%: 5,1%-5,7% dos estudantes relataram ter sofrido bullying quase sempre ou sempre nos últimos 30 dias; 25,4% (IC95%: 24,8%-26,0% foram raramente ou às vezes vítimas de bullying e 69,2% (IC95%: 68,5%-69,8% não sentiram nenhuma humilhação ou provocação (bullying. A capital com maior frequência de foi Belo Horizonte-MG (6,9%; IC95%: 5,9%-7,9 e a menor foi Palmas-TO (3,5%; IC95%: 2,6%-4,5%. Meninos relatam mais bullying (6,0%; IC95%: 5,5%-6,5% do que meninas (4,8%; IC95%: 4,4%-5,3%. Não houve diferença entre escolas públicas (5,5%; IC95%: 5,1%-5,8% e privadas (5,2%; IC95%: 4,6%-5,8%, exceto em Aracaju-SE, onde foi registrada maior ocorrência de bullying em escolas privadas. Os dados mostram urgente necessidade de ações intersetoriais a partir de políticas e práticas educativas que efetivem redução e prevenção da ocorrência do bullying nas escolas.

  10. Evaluation of tumour markers as differential diagnostic tool in patients with suspicion of liver metastases from breast cancer.

    Science.gov (United States)

    Liska, Vaclav; Holubec, Lubos; Treska, Vladislav; Vrzalova, Jindra; Skalicky, Tomas; Sutnar, Alan; Kormunda, Stanislav; Bruha, Jan; Vycital, Ondrej; Finek, Jindrich; Pesta, Martin; Pecen, Ladislav; Topolcan, Ondrej

    2011-04-01

    The liver is the site of breast cancer metastasis in 50% of patients with advanced disease. Tumour markers have been demonstrated as being useful in follow-up of patients with breast cancer, in early detection of recurrence of breast cancer after radical surgical treatments, and in assessing oncologic therapy effect, but no study has been carried out on their usefullness in distinguishing benign liver lesions from breast cancer metastases. The aim of this study was therefore to evaluate the importance of tumour markers carcinoembryonic antigen (CEA), carbohydrate antigen CA19-9 (CA19-9), thymidine kinase (TK), tissue polypeptide antigen (TPA), tissue polypeptide-specific antigen (TPS) and cytokeratin 19 fragment (CYFRA 21-1) in differential diagnosis between benign liver lesions and liver metastases of breast cancer. The study includes 3 groups: 22 patients with liver metastases of breast cancer; 39 patients with benign liver lesions (hemangioma, focal nodular hyperplasia, liver cyst, hepatocellular adenoma); and 21 patients without any liver disease or lesion that were operated on for benign extrahepatic diseases (groin hernia, varices of lower limbs) as a control group. The serum levels of tumour markers were assessed by means of immunoanalytical methods. Preoperative serum levels of CYFRA 21-1, TPA, TPS and CEA were significantly higher in patients with liver metastases of breast cancer in contrast to healthy controls and patients with benign liver lesions (p-value<0.05). Serum levels of CA19-9 and TK were higher in patients with malignancy in comparison with benign liver disease and healthy controls but these differences were not statistically significant. Tumour markers CEA, CYFRA 21-1, TPA and TPS can be recommended as a good tool for differential diagnosis between liver metastases of breast cancer and benign liver lesions.

  11. Analysis of the discriminative methods for diagnosis of benign and malignant solitary pulmonary nodules based on serum markers.

    Science.gov (United States)

    Wang, Wanping; Liu, Mingyue; Wang, Jing; Tian, Rui; Dong, Junqiang; Liu, Qi; Zhao, Xianping; Wang, Yuanfang

    2014-01-01

    Screening indexes of tumor serum markers for benign and malignant solitary pulmonary nodules (SPNs) were analyzed to find the optimum method for diagnosis. Enzyme-linked immunosorbent assays, an automatic immune analyzer and radioimmunoassay methods were used to examine the levels of 8 serum markers in 164 SPN patients, and the sensitivity for differential diagnosis of malignant or benign SPN was compared for detection using a single plasma marker or a combination of markers. The results for serological indicators that closely relate to benign and malignant SPNs were screened using the Fisher discriminant analysis and a non-conditional logistic regression analysis method, respectively. The results were then verified by the k-means clustering analysis method. The sensitivity when using a combination of serum markers to detect SPN was higher than that using a single marker. By Fisher discriminant analysis, cytokeratin 19 fragments (CYFRA21-1), carbohydrate antigen 125 (CA125), squamous cell carcinoma antigen (SCC) and breast cancer antigen (CA153), which relate to the benign and malignant SPNs, were screened. Through non-conditional logistic regression analysis, CYFRA21-1, SCC and CA153 were obtained. Using the k-means clustering analysis, the cophenetic correlation coefficient (0.940) obtained by the Fisher discriminant analysis was higher than that obtained with logistic regression analysis (0.875). This study indicated that the Fisher discriminant analysis functioned better in screening out serum markers to recognize the benign and malignant SPN. The combined detection of CYFRA21-1, CA125, SCC and CA153 is an effective way to distinguish benign and malignant SPN, and will find an important clinical application in the early diagnosis of SPN. © 2014 S. Karger GmbH, Freiburg.

  12. Predictive and prognostic value of circulating nucleosomes and serum biomarkers in patients with metastasized colorectal cancer undergoing Selective Internal Radiation Therapy

    International Nuclear Information System (INIS)

    Fahmueller, Yvonne Nadine; Nagel, Dorothea; Hoffmann, Ralf-Thorsten; Tatsch, Klaus; Jakobs, Tobias; Stieber, Petra; Holdenrieder, Stefan

    2012-01-01

    Selective Internal Radiation Therapy (SIRT) is a new and effective locoregional anticancer therapy for colorectal cancer patients with liver metastases. Markers for prediction of therapy response and prognosis are needed for the individual management of those patients undergoing SIRT. Blood samples were prospectively and consecutively taken from 49 colorectal cancer patients with extensive hepatic metastases before, three, six, 24 and 48 h after SIRT to analyze the concentrations of nucleosomes and further laboratory parameters, and to compare them with the response to therapy regularly determined 3 months after therapy and with overall survival. Circulating nucleosomes, cytokeratin-19 fragments (CYFRA 21-1), carcinoembryonic antigen (CEA), C-reactive protein (CRP) and various liver markers increased already 24 h after SIRT. Pretherapeutical levels of CYFRA 21-1, CEA, cancer antigen 19-9 (CA 19-9), asparate-aminotransferase (AST) and lactate dehydrogenase (LDH) as well as 24 h values of nucleosomes were significantly higher in patients suffering from disease progression (N = 35) than in non-progressive patients (N = 14). Concerning overall survival, CEA, CA 19-9, CYFRA 21-1, CRP, LDH, AST, choline esterase (CHE), gamma-glutamyl-transferase, alkaline phosphatase, and amylase (all 0 h, 24 h) and nucleosomes (24 h) were found to be prognostic relevant markers in univariate analyses. In multivariate Cox-Regression analysis, the best prognostic model was obtained for the combination of CRP and AST. When 24 h values were additionally included, nucleosomes (24 h) further improved the existing model. Panels of biochemical markers are helpful to stratify pretherapeutically colorectal cancer patients for SIR-therapy and to early estimate the response to SIR-therapy

  13. EMPIRICAL TESTING OF MODIFIED BLACK-SCHOLES OPTION PRICING MODEL FORMULA ON NSE DERIVATIVE MARKET IN INDIA

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    Ambrish Gupta

    2013-01-01

    Full Text Available The main objectives of this paper are to incorporate modification in Black-Scholes option pricing model formula by adding some new variables on the basis of given assumption related to risk-free interest rate, and also shows the calculation process of new risk-free interest rate on the basis of modified variable. This paper also identifies the various situations in empirical testing of modified and original Black-Scholes formula with respect to the market value on the basis of assumed and calculated risk-free interest rate.

  14. Avaliação dos marcadores tumorais antígeno carcinoembrionário, fragmento de citoqueratina 19 e antígeno associado ao câncer 72-4 na diferenciação de efusões neoplásicas e não neoplásicas caninas

    OpenAIRE

    Teixeira, L.V.; Guerra, T.A.; Conrado, F.O.; Terra, S.R.; Gerardi, D.G.; González, F.H.D.

    2014-01-01

    The concentration of tumor markers in body fluids can be used for diagnosis and prognosis of patients. This study aimed to investigate the performance of tumor markers cytokeratin 19 fragment (CYFRA 21-1), cancer-associated antigen 72-4 (CA 72-4) and carcinoembryonic antigen (CEA) in the neoplastic and non-neoplastic canine effusions. In thirty-two neoplastic (n=16) and non-neoplastic (n=16) samples of canine thoracic or abdominal effusions, tumor markers were measured. Significant statistica...

  15. [Correlation between Serum Tumor Markers and Efficacy of First-line EGFR-TKIs in Patients with Advanced Lung Adenocarcinoma].

    Science.gov (United States)

    Chen, Hanxiao; Yang, Xue; Liu, Huijun; Ma, Kun; Zhong, Jia; Dong, Zhi; Zhuo, Minglei; Wang, Yuyan; Li, Jianjie; An, Tongtong; Wu, Meina; Wang, Ziping; Zhao, Jun

    2017-09-20

    Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) significantly improve the survival of advanced lung adenocarcinoma patients harboring EGFR mutation. Limited to the standards of tumor tissue samples and detection methods, still some people can't receive target therapy following genetic guidance. This study was to explore the relevance between serum tumor markers and treatment of EGFR-TKIs. We retrospectively collected the clinical information of advanced lung adenocarcinoma patients harboring EGFR mutation, who received EGFR-TKIs as first-line therapy from June 2009 to June 2014 in Peking University Cancer Hospital, analyzed the relationship between serum tumor markers and efficacy of EGFR-TKIs. The objective response rate (ORR) was 52.8% and the disease control rate (DCR) was 89.3%. The results showed that, patients with high CEA level before treatment responded better to TKIs (ORR 61.3% vs 35.9%, DCR 95.2% vs 74.4%, PCEA decreased 1 month later (61.5% vs 25%, P=0.002). Progression-free survival (PFS) significantly prolonged in patients with elevated baseline CEA (mPFS 9.8 mo vs 5.9 mo, P=0.027). To the opposite, PFS was significantly shorter in patients with elevated baseline CYFRA21-1 and CA125 (mPFS 9.0 mo vs 11.4 mo, P=0.029; 9.0 mo vs 11.5 mo, P=0.023, respectively). Multivariate analysis showed that Eastern Cooperative Oncology Group (ECOG) score of 0-1, normal baseline CYFRA21-1 and CEA decline predicted longer PFS. The overall survival (OS) was highly associated with elevated CYFRA21-1 and CA125 (median OS 25.1 mo vs 52.5 mo, P=0.003; 22.7 mo vs 55.0 mo, PCEA. High level of baseline CEA and decline 1 month after treatment could predict the efficacy of EGFR-TKIs in patients with advanced lung adenocarcinoma. While high levels of baseline CYFRA21-1 and CA125 indicated shortened survival.

  16. Direct detection of cancer biomarkers in blood using a "place n play" modular polydimethylsiloxane pump.

    Science.gov (United States)

    Zhang, Honglian; Li, Gang; Liao, Lingying; Mao, Hongju; Jin, Qinghui; Zhao, Jianlong

    2013-01-01

    Cancer biomarkers have significant potential as reliable tools for the early detection of the disease and for monitoring its recurrence. However, most current methods for biomarker detection have technical difficulties (such as sample preparation and specific detector requirements) which limit their application in point of care diagnostics. We developed an extremely simple, power-free microfluidic system for direct detection of cancer biomarkers in microliter volumes of whole blood. CEA and CYFRA21-1 were chosen as model cancer biomarkers. The system automatically extracted blood plasma from less than 3 μl of whole blood and performed a multiplex sample-to-answer assay (nano-ELISA (enzyme-linked immunosorbent assay) technique) without the use of external power or extra components. By taking advantage of the nano-ELISA technique, this microfluidic system detected CEA at a concentration of 50 pg/ml and CYFRA21-1 at a concentration of 60 pg/ml within 60 min. The combination of PnP polydimethylsiloxane (PDMS) pump and nano-ELISA technique in a single microchip system shows great promise for the detection of cancer biomarkers in a drop of blood.

  17. Can tumour marker assays be a guide in the prescription of bone scan for breast and lung cancers?

    Energy Technology Data Exchange (ETDEWEB)

    Buffaz, P.-D.; Gauchez, A.S.; Caravel, J.P.; Vuillez, J.P.; Cura, C.; Agnius-Delord, C.; Fagret, D. [Service de Medecine Nucleaire, Centre Hospitalier Universitaire de Grenoble (France)

    1999-01-01

    Considering the current need to improve cost-effectiveness in cancer patient management, a prospective study was undertaken in order to define the optimal combination of bone scan and tumour marker assays in breast and lung cancer strategies, as has been done in the case of prostate cancer. All patients with breast or lung cancer referred to the Nuclear Medicine Department of the Grenoble Teaching Hospital between December 1995 and April 1997 were included. A blood sample was drawn in each case for marker assay (CA15-3 or CEA and CYFRA 21-1) on the same day as the bone scan. Two hundred and seventy-five patients were included: 118 with lung cancer and 157 with breast cancer. With regard to lung cancer, no information useful for guiding bone scan prescription was obtained through CEA and CYFRA 21-1 assays. For breast cancer, the results suggest that in asymptomatic patients, a CA15-3 level of less than 25 U/ml (upper normal value chosen as the threshold) is strongly predictive of a negative bone scan; by contrast, high tumour marker levels are predictive of neoplastic bone involvement. When a doubtful bone scan is obtained in a patient with breast cancer, a normal marker level makes it highly probable that bone scan abnormalities are not related to malignancy. (orig.) With 3 figs., 21 refs.

  18. L-cysteine capped lanthanum hydroxide nanostructures for non-invasive detection of oral cancer biomarker.

    Science.gov (United States)

    Tiwari, Sachchidanand; Gupta, Pramod K; Bagbi, Yana; Sarkar, Tamal; Solanki, Pratima R

    2017-03-15

    In this paper, we present the result of studies related to the in situ synthesis of amino acid (L-Cysteine) capped lanthanum hydroxide nanoparticles [Cys-La(OH) 3 NPs] towards the fabrication of efficient immunosensor for non-invasive detection of oral cancer. The characterization of Cys-La(OH) 3 NPs was carried out by different techniques including X-ray diffraction, scanning electron microscopy, transmission electron microscopy, fourier transform infrared spectroscopy and electrochemical techniques. These Cys-La(OH) 3 NPs were electrophoretically deposited onto an indium-tin-oxide glass substrate and used for immobilization of anti-cytokeratin fragment-21-1 (anti-Cyfra-21-1) for the electrochemical detection of Cyfra-21-1. This immunosensor shows a broad detection range of 0.001-10.2ngmL -1 , the low detection limit of 0.001ngmL -1 , and high sensitivity of 12.044µA (ng per mL cm -2 ) -1 with a response time of 5min. This immunosensor was found to be more advanced in terms of high sensitivity and low detection limit as compared to previously reported biosensors and commercially available ELISA kit (Kinesis DX). Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Application of serum microRNA-9-5p, 21-5p, and 223-3p combined with tumor markers in the diagnosis of non-small-cell lung cancer in Yunnan in southwestern China.

    Science.gov (United States)

    Yang, Yanlong; Chen, Kai; Zhou, Yongchun; Hu, Zaoxiu; Chen, Shuai; Huang, Yunchao

    2018-01-01

    Xuanwei City is located in late Permian coal-accumulating areas of the northeastern region of Yunnan Province. In China, morbidity and mortality from lung cancer are highest in Yunnan. Identifying useful circulating markers suitable for the diagnosis of lung cancer in this region is quite meaningful. In this study, we evaluated diagnostic roles of serum miR-9-5p, 21-5p, 223-3p, 135b-5p, 339-5p, and 501-5p in patients with non-small-cell lung cancer (NSCLC) in Yunnan. Moreover, we evaluated the diagnostic performance of several tumor markers, including carcinoembryonic antigen (CEA), cytokeratin 19 fragment 21-1 (CYFRA21-1), and squamous cell carcinoma-related antigen (SCC). Quantitative real-time polymerase chain reaction detected six miRNAs in the serum of 104 NSCLC patients and 50 cancer-free controls. Other markers, including CEA, CYFRA21-1, and SCC, in serum were also measured. The diagnostic ability of miRNAs and tumor markers was evaluated by receiver operating characteristic (ROC) curve analysis. The diagnostic performance of these serum markers was also evaluated in Xuanwei and non-Xuanwei subjects, because the etiological and the epidemiological characteristics of lung cancer in Xuanwei were quite different from those in other regions. Serum miR-9-5p, miR-21-5p, miR-223-3p, CEA, CYFRA21-1, and SCC were upregulated in NSCLC patients, compared with cancer-free controls. No significant difference was found in miR-135b-5p, miR-339-5p, and miR-501-5p expression. The area under ROC curves (AUCs) of miR-9-5p, miR-21-5p, miR-223-3p, CEA, CYFRA21-1, and SCC were 0.706, 0.765, 0.744, 0.749, 0.735, and 0.616, respectively. When combined, miRNAs and tumor markers yielded the highest diagnostic power, with AUC of 0.886, sensitivity of 82.69%, and specificity of 88.00%. In Xuanwei subjects, miR-223-3p and CEA may be suitable biomarkers to distinguish NSCLC from cancer-free states with AUCs of 0.752 and 0.791, respectively. The diagnostic power of the combination of mi

  20. Squamous Cell Carcinoma Antigen: A Novel Tumor Marker for Hepatocellular Carcinoma

    International Nuclear Information System (INIS)

    Abdel Messeih, Ph.L.

    2009-01-01

    Serum Squamous Cell Carcinoma Antigen (SCC-Ag) by ELISA technique and Alpha-fetoprotein (AFP) by IRMA technique were measured in 65 patients with hepatic focal lesion. 49 patients suffered from proved hepatocellular carcinoma and 16 patients were having cirrhosis and 20 normal controls. Median levels of serum AFP and SCC-Ag in HCC patients was significantly higher when compared with both cirrhotic patients and controls. On using receiver operator characteristic curve to improve sensitivity and specificity of AFP and SCC-Ag for detection of HCC, the best chosen cut-off values were 40 IU/mL for AFP and 2.55 ng/L for SCC-Ag, these yielded a sensitivity of 67.2% and 61.2% respectively and specificity 100%. The diagnostic sensitivity of them increased to 87.7% when they was combiendly calculated. It was found that the combined use of AFP and SCC-Ag is useful in screening patients with hepatic focal lesion to increase the chance of early diagnosis of HCC patients.

  1. Psychoactive substance use, family context and mental health among Brazilian adolescents, National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To evaluate the association between the consumption of psychoactive substances (tobacco, alcohol and illicit drugs and demographic variables, mental health and family context among school-aged children. METHODS: The National Adolescent School-based Health Survey was held with a national sample of 109,104 students. Data regarding demographic variables, family background and mental health were collected. Logistic regression was used to evaluate the associations of interest. RESULTS: Multivariate analyses showed that alcohol consumption was higher among girls, drug experimentation was more frequent among boys and that there was no difference between sexes for smoking. Being younger and mulatto were negatively associated with the use of tobacco, alcohol and illicit drugs. Also negatively associated with such risk behaviors were characteristics of the family context represented by: living with parents, having meals together and parental supervision (when parents know what the child does in their free time. Moreover, characteristics of mental health such as loneliness and insomnia were positively associated with use of tobacco, alcohol and illicit drugs. Not having friends was positively associated with use of tobacco and illicit drugs and negatively associated with alcohol use. CONCLUSIONS: The study shows the protective effect of family supervision in the use of tobacco, alcohol and drugs and, on the contrary, the increasing use of substances according to aspects of mental health, such as loneliness, insomnia and the fact of not having friends. The study's findings may support actions from health and education professionals, as well as from the government and families in order to prevent the use of these substances by adolescents.

  2. Labor in early life, vulnerability for health in Brazilian schoolchildren: National Adolescent School-based Health Survey (PeNSE 2012

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    Luana Giatti

    2014-01-01

    Full Text Available OBJECTIVE: This study describes exposure labor among Brazilian 9th grade students from public and private schools and investigates socio-demographic characteristics, behaviors, violent situations and psychosocial factors associated with labor among adolescents. METHODS: The present study included 108,984 students from the National Adolescent School-based Health Survey carried out in 2012. Variables were grouped into sociodemographic characteristics, behavioral factors, violent situations and psychosocial aspects. Associations between labor and several health risk variables were identified by multiple logistic regression analysis, after adjustment for sex and age. RESULTS: Approximately 13% of the students reported having a job: 17.4% of them were male. The chance of working was lower between females and individuals whose fathers' have incomplete superior education. Students who worked had greater chances to smoke (OR = 2.26; 95%CI 2.04 - 2.51, drink alcohol, use illicit drugs (OR = 2.63; 95%CI 2.29 - 3.02, drive motorized vehicles (OR = 2.15; 95%CI 2.03 2.27, have sexual intercourse (OR = 2.10; 95%CI 1.99 - 2.24, suffer physical violence (OR = 1.57; 95%CI 1.46 1.68, engage in fights (OR = 1.65; 95%CI 1.55 - 1.76, feel lonely (OR = 1.26; 95%CI 1.17 - 1.36 and report sleeping problems (OR = 1.46; 95%CI 1.34 - 1.60. They also have lower chances of having close friends (OR = 0.78; 95%CI 0.68 - 0.90. CONCLUSION: The prevalence of labor among students is high. Socioeconomical disadvantages increase the chances of early working. Early working is also associated to health damaging behavior, violent situations, sleeping problems, and social isolation. Adolescents who study and work experiment expositions that may affect distinct health dimensions and perpetuate disadvantages over lifetime.

  3. Psychoactive substance use, family context and mental health among Brazilian adolescents, National Adolescent School-based Health Survey (PeNSE 2012).

    Science.gov (United States)

    Malta, Deborah Carvalho; Oliveira-Campos, Maryane; do Prado, Rogério Ruscitto; Andrade, Silvania Suely Caribé; de Mello, Flávia Carvalho Malta; Dias, Antonio José Ribeiro; Bomtempo, Denise Birche

    2014-01-01

    To evaluate the association between the consumption of psychoactive substances (tobacco, alcohol and illicit drugs) and demographic variables, mental health and family context among school-aged children. The National Adolescent School-based Health Survey was held with a national sample of 109,104 students. Data regarding demographic variables, family background and mental health were collected. Logistic regression was used to evaluate the associations of interest. Multivariate analyses showed that alcohol consumption was higher among girls, drug experimentation was more frequent among boys and that there was no difference between sexes for smoking. Being younger and mulatto were negatively associated with the use of tobacco, alcohol and illicit drugs. Also negatively associated with such risk behaviors were characteristics of the family context represented by: living with parents, having meals together and parental supervision (when parents know what the child does in their free time). Moreover, characteristics of mental health such as loneliness and insomnia were positively associated with use of tobacco, alcohol and illicit drugs. Not having friends was positively associated with use of tobacco and illicit drugs and negatively associated with alcohol use. The study shows the protective effect of family supervision in the use of tobacco, alcohol and drugs and, on the contrary, the increasing use of substances according to aspects of mental health, such as loneliness, insomnia and the fact of not having friends. The study's findings may support actions from health and education professionals, as well as from the government and families in order to prevent the use of these substances by adolescents.

  4. Situations of violence experienced by students in the state capitals and the Federal District: results from the National Adolescent School-based Health Survey (PeNSE 2012).

    Science.gov (United States)

    Malta, Deborah Carvalho; Mascarenhas, Márcio Dênis Medeiros; Dias, Antônio Ribeiro; do Prado, Rogério Ruscitto; Lima, Cheila Marina; da Silva, Marta Maria Alves; da Silva Júnior, Jarbas Barbosa

    2014-01-01

    To describe violent events experienced by school-aged adolescents in school, around the school and in the family context, and to compare the results of the National Adolescent School-based Health Survey of 2009 and 2012. Indicators related to violence involving teenagers were analyzed. The prevalence rates and confidence intervals of 95% were calculated for events of interest according to gender and type of school (public or private) and regions. Prevalence rates were: insecurity in the route between home-school (9.1%), insecurity in school (8.0%), physical assault in the last 12 months (18.2%), engaging in fights in the last 12 months (20.7%), fighting with a cold weapon (8.3%), fighting with firearms (6.9%), physical assault by a family member (11.6%) and having been seriously injured in the last 12 months (10.3%). The situations of violence were more prevalent among male students from public schools. The comparison with the 2009 survey showed increased prevalence in all the variables studied. Teenagers are exposed to different forms of violence, and the data from the National Survey of School Health can support the planning of preventive actions.

  5. Trend of the risk and protective factors of chronic diseases in adolescents, National Adolescent School-based Health Survey (PeNSE 2009 e 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To compare the prevalence of major risk and protection factors for chronic non-communicable diseases in school-aged children in Brazilian capitals surveyed in the National Adolescent School-based Health Survey in its two editions, 2009 and 2012. METHODS: The frequencies, with Confidence Interval of 95%, of the following demographic variables were compared: food intake, body image, physical activity, smoking, alcohol and other drugs. Prevalence was compared in the two editions of the survey. RESULTS: The proportion of students who attend two physical education classes a week was maintained at 49% between 2009 and 2012, increasing in public schools from 50.6% (95%CI 49.8 - 51.4 to 52.5% (95%CI 49.2 - 55.7, and decreasing in private schools. There was no change in the proportion of students who watch two hours or more of television daily, about 80%. As for body image, there was no change between the two editions, and about 60% considered themselves being of normal weight. There was a reduction in the percentage of adolescents who experienced cigarettes, from 24.2% (95%CI 23.6 - 24.8 to 22.3% (95%CI 21.4 - 23.2, and the prevalence of smoking was maintained at about 6% (there was no statistical difference between 2009 and 2012. The consumption of beans, fruits, sweets and soft drinks also decreased. Frequency of drug experimentation was of 8.7% (95%CI 8.3 - 9.1 in 2009, and 9.6% (95%CI 9.0 - 10.3 in 2012, with no difference between confidence intervals, and the frequency of alcohol experimentation was maintained at about 70%; the percentage of use in the past 30 days was also maintained at around 27%. CONCLUSION: In the Brazilian capitals, the vast majority of prevalence of risk factors were kept stable in the two editions of the National Survey of School. These data generate evidence to guide the implementation of public policies to minimize the exposure of adolescents to risk factors.

  6. Bullying and associated factors among Brazilian adolescents: analysis of the National Adolescent School-based Health Survey (PeNSE 2012).

    Science.gov (United States)

    Malta, Deborah Carvalho; do Prado, Rogério Ruscitto; Dias, Antônio José Ribeiro; Mello, Flavia Carvalho M; Silva, Marta Angelica Iossi; da Costa, Michelle Ralil; Caiaffa, Waleska Teixeira

    2014-01-01

    To estimate the prevalence of bullying from the victim's perspective in Brazilian school children and to analyze its association with individual and family context variables. An analysis of the data on 109,104 adolescents, obtained by the National Adolescent School-based Health Survey, held in schools in 2012, was carried out. An association model between bullying and explanatory variables was tested in different contexts: sociodemographic, risk behaviors, mental health and family context. Univariate and multivariate analyzes were performed, calculating the Odds Ratio and confidence intervals. The prevalence of bullying found in this study was of 7.2% (95%CI 6.6 - 7.8). A higher chance of bullying was found among male students (OR = 1.58; 95%CI 1.51 - 1.66), with an inverse relation between age and bullying, with the magnitude of risk among adolescents younger than 13 years of age being higher when compared to those with 16 years of age or more. Of individual risk behaviors, only being a smoker remained in the final model (OR = 1.11; 95%CI 1.01 - 1.23). Mental health variables associated with bullying were: feeling lonely (OR = 2.66; 95%CI 2.52 - 2.81), insomnia (OR = 1.92; 95%CI 1.80 - 2.05), not having friends (OR = 1.71; 95%CI 1.54 - 1.89), and, in the family context, those who skip class without telling their parents (OR = 1.13; 95%CI 1,07 - 1,19) and those who suffer physical abuse by family members (OR = 2.03; 95%CI 1.91 - 2.146). Bullying was associated to male students, younger, of black color, smokers, with mental health vulnerabilities and victims of domestic violence. This suggests the need for a holistic approach from education and health professionals, parents and the community in seeking measures for the prevention of bullying.

  7. Bullying and associated factors among Brazilian adolescents: analysis of the National Adolescent School-based Health Survey (PeNSE 2012

    Directory of Open Access Journals (Sweden)

    Deborah Carvalho Malta

    2014-01-01

    Full Text Available OBJECTIVE: To estimate the prevalence of bullying from the victim's perspective in Brazilian school children and to analyze its association with individual and family context variables. METHODS: An analysis of the data on 109,104 adolescents, obtained by the National Adolescent School-based Health Survey, held in schools in 2012, was carried out. An association model between bullying and explanatory variables was tested in different contexts: sociodemographic, risk behaviors, mental health and family context. Univariate and multivariate analyzes were performed, calculating the Odds Ratio and confidence intervals. RESULTS: The prevalence of bullying found in this study was of 7.2% (95%CI 6.6 - 7.8. A higher chance of bullying was found among male students (OR = 1.58; 95%CI 1.51 - 1.66, with an inverse relation between age and bullying, with the magnitude of risk among adolescents younger than 13 years of age being higher when compared to those with 16 years of age or more. Of individual risk behaviors, only being a smoker remained in the final model (OR = 1.11; 95%CI 1.01 - 1.23. Mental health variables associated with bullying were: feeling lonely (OR = 2.66; 95%CI 2.52 - 2.81, insomnia (OR = 1.92; 95%CI 1.80 - 2.05, not having friends (OR = 1.71; 95%CI 1.54 - 1.89, and, in the family context, those who skip class without telling their parents (OR = 1.13; 95%CI 1,07 - 1,19 and those who suffer physical abuse by family members (OR = 2.03; 95%CI 1.91 - 2.146. CONCLUSION: Bullying was associated to male students, younger, of black color, smokers, with mental health vulnerabilities and victims of domestic violence. This suggests the need for a holistic approach from education and health professionals, parents and the community in seeking measures for the prevention of bullying.

  8. Aplicação clínica dos marcadores tumorais séricos em carcinoma não-pequenas células do pulmão

    OpenAIRE

    GROSS JEFFERSON LUIZ; YOUNES RIAD NAIM; BARBUTO JOSÉ ALEXANDRE MARZAGÃO; HADDAD FABIO JOSÉ; DEHENZELIN DANIEL

    2000-01-01

    O valor clínico dos marcadores tumorais séricos em câncer de pulmão é incerto. Objetivos: Avaliar a associação da concentração sérica de marcadores tumorais com a extensão da neoplasia e seu valor prognóstico. Casuística e método: Entre fevereiro de 1995 e setembro de 1997 foram estudados 103 pacientes, no Departamento de Cirurgia Torácica do Hospital do Câncer. Antes do tratamento os pacientes foram submetidos à coleta de sangue para dosagem da concentração do CEA, CYFRA21.1, CA15.3, CA19.9,...

  9. Prognostic impact of cytological fluid tumor markers in non-small cell lung cancer.

    Science.gov (United States)

    Cho, Arthur; Hur, Jin; Hong, Yoo Jin; Lee, Hye-Jeong; Kim, Young Jin; Hong, Sae Rom; Suh, Young Joo; Im, Dong Jin; Kim, Yun Jung; Lee, Jae Seok; Shim, Hyo Sup; Choi, Byoung Wook

    2016-03-01

    The serum tumor markers CYFRA 21-1, carcinoembryonic antigen (CEA), and squamous cell carcinoma antigen (SCCA) are useful in diagnosis and prognosis of non-small cell lung cancer (NSCLC). Cytologic tumor markers obtained during needle aspiration biopsies (NAB) of lung lesions are useful for NSCLC diagnosis. This study investigated the incremental prognostic value of cytologic tumor markers compared to serum tumor markers. This prospective study included 253 patients diagnosed with NSCLC by NAB with cytologic tumor marker analysis. Levels of cytologic CYFRA 21-1, CEA, SCCA, and their serum counterparts were followed up for survival analysis. Optimal cutoff values for each tumor marker were obtained for overall survival (OS) and progression-free survival (PFS) analyses. All patients were followed up for a median of 22.8 months. Using cutoff values of 0.44 ng/ml for C-SCCA, 2.0 ng/ml for S-SCCA, and 3.3 ng/ml for S-CYFRA, a multivariate analysis revealed that high S-SCCA (hazard ratio, HR, 1.84) and high C-SCCA (HR, 1.63) were independent predictive factors of OS. The 3-year overall survival rate was 55 vs. 80 % for high and low C-SCCA, respectively. Cytologic tumor marker level detection is easily obtainable and provides prognostic information for NSCLC. Cytologic tumor markers provide comparable prognostic information relative to serum tumor markers, with C-SCCA acting as a strong prognostic factor of overall survival and PFS.

  10. Clinical study on bevacizumab combined with carboplatin therapy for malignant pleural effusion of non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    Li-Ping Yang1

    2017-06-01

    Full Text Available Objective: To investigate the effect of bevacizumab combined with carboplatin therapy for malignant pleural effusion of non-small cell lung cancer on tumor markers, angiogenesis molecules and invasive growth molecules. Methods: A total of 68 patients who were diagnosed with non-small cell lung cancer complicated by pleural effusion in the Affiliated T.C.M Hospital of Southwest Medical University between June 2013 and August 2016 were selected and randomly divided into two groups, the combined group received bevacizumab combined with carboplatin chemotherapy, and the carboplatin group received carboplatin chemotherapy. Before treatment as well as 3 cycles and 6 cycles after treatment, the contents of tumor markers, angiogenesis molecules and invasive growth molecules in pleural effusion were examined. Results: 3 cycles and 6 cycles after treatment, CEA, SCCAg, CYFRA21-1, sHLA-G, VEGF, VEGFR, PTN, MMP7 and MMP10 contents in pleural effusion of both groups of patients were significantly lower than those before treatment while TIMP1 and TIMP2 contents were significantly higher than those before treatment, and CEA, SCCAg, CYFRA21-1, sHLA-G, VEGF, VEGFR, PTN, MMP7 and MMP10 contents in pleural effusion of combined group were significantly lower than those of carboplatin group while TIMP1 and TIMP2 contents were significantly higher than those of carboplatin group. Conclusion: Bevacizumab combined with carboplatin therapy for malignant pleural effusion of non-small cell lung cancer can effectively kill cancer cells, and inhibit angiogenesis and cell invasion.

  11. Evaluation of a CLEIA automated assay system for the detection of a panel of tumor markers.

    Science.gov (United States)

    Falzarano, Renato; Viggiani, Valentina; Michienzi, Simona; Longo, Flavia; Tudini, Silvestra; Frati, Luigi; Anastasi, Emanuela

    2013-10-01

    Tumor markers are commonly used to detect a relapse of disease in oncologic patients during follow-up. It is important to evaluate new assay systems for a better and more precise assessment, as a standardized method is currently lacking. The aim of this study was to assess the concordance between an automated chemiluminescent enzyme immunoassay system (LUMIPULSE® G1200) and our reference methods using seven tumor markers. Serum samples from 787 subjects representing a variety of diagnoses, including oncologic, were analyzed using LUMIPULSE® G1200 and our reference methods. Serum values were measured for the following analytes: prostate-specific antigen (PSA), alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), carbohydrate antigen 15-3 (CA15-3), carbohydrate antigen 19-9 (CA19-9), and cytokeratin 19 fragment (CYFRA 21-1). For the determination of CEA, AFP, and PSA, an automatic analyzer based on chemiluminescence was applied as reference method. To assess CYFRA 21-1, CA125, CA19-9, and CA15-3, an immunoradiometric manual system was employed. Method comparison by Passing-Bablok analysis resulted in slopes ranging from 0.9728 to 1.9089 and correlation coefficients from 0.9977 to 0.9335. The precision of each assay was assessed by testing six serum samples. Each sample was analyzed for all tumor biomarkers in duplicate and in three different runs. The coefficients of variation were less than 6.3 and 6.2 % for within-run and between-run variation, respectively. Our data suggest an overall good interassay agreement for all markers. The comparison with our reference methods showed good precision and reliability, highlighting its usefulness in clinical laboratory's routine.

  12. Comparison of LUMIPULSE(®) G1200 With Kryptor and Modular E170 for the Measurement of Seven Tumor Markers.

    Science.gov (United States)

    Marlet, Julien; Bernard, Maguy

    2016-01-01

    Tumor marker measurements are becoming essential for prognosis and follow-up of patients in oncology. In this context, we aimed to compare a new analyzer, Lumipulse(®) G1200 (Fujirebio group, distributed in Europe by the Innogenetics group) with Kryptor(®) (Thermo Fisher Scientific B.R.A.H.M.S, Asnières, France) and Modular(®) Elecsys E170 (Roche Diagnostics, Meylan, France) for the measurement of seven tumor markers: PSA, AFP, CEA, CA 15-3, CA 125, CA 19-9, and Cyfra 21-1. A total of 471 serum samples from patients with elevated tumor markers and 100 serum from healthy patients were analyzed with Lumipulse(®) G1200 and either Kryptor(®) (for AFP) or Modular(®) (for the six other markers). The good precision of Lumipulse(®) G1200 assays was confirmed with CVs Lumipulse results were well correlated with Modular or Kryptor results (r ≥ 0.94). Concordance of results interpretation was > 95% and tumor marker kinetics were all similar. We confirmed the analytical performances of Lumipulse(®) tumor marker assays except for the CYFRA 21-1 assay for which performances were poor in this study. We noticed a few discrepancies for the CEA assay. Besides, values obtained for CA 19-9 were higher with Lumipulse leading to a bias (slope = 1.5). But for the four other tumor markers assays (PSA, AFP, CA 125, CA 15-3), the results were directly transferable between Lumipulse and Kryptor or Modular, thus facilitating an eventual substitution of one system by another. © 2014 Wiley Periodicals, Inc.

  13. Polarimetric neutron spin echo: Feasibility and first results

    Energy Technology Data Exchange (ETDEWEB)

    Pappas, C. [Hahn-Meitner Institut Berlin, Glienickerstr. 100, 14109 Berlin (Germany)], E-mail: pappas@hmi.de; Lelievre-Berna, E. [Institut Laue-Langevin, 6, Rue Jules Horowitz, 38042 Grenoble (France); Bentley, P. [Hahn-Meitner Institut Berlin, Glienickerstr. 100, 14109 Berlin (Germany); Bourgeat-Lami, E. [Institut Laue-Langevin, 6, Rue Jules Horowitz, 38042 Grenoble (France); Moskvin, E. [Hahn-Meitner Institut Berlin, Glienickerstr. 100, 14109 Berlin (Germany); PNPI, 188300 Gatchina, Leningrad District (Russian Federation); Thomas, M. [Institut Laue-Langevin, 6, Rue Jules Horowitz, 38042 Grenoble (France); Grigoriev, S.; Dyadkin, V. [PNPI, 188300 Gatchina, Leningrad District (Russian Federation)

    2008-07-21

    Neutron Spin Echo (NSE) spectroscopy uses polarized neutrons and accordingly polarization analysis is an intrinsic feature of NSE. However, the multifaceted dynamics of antiferromagnets and helimagnets require more than the classical NSE set-up. Here we present the feasibility test and first results of a new and powerful technique: Polarimetric NSE, obtained by combining the wide angle NSE spectrometer SPAN, developed at HMI with the zero-field polarimeter Cryopad developed at ILL.

  14. Polarimetric neutron spin echo: Feasibility and first results

    International Nuclear Information System (INIS)

    Pappas, C.; Lelievre-Berna, E.; Bentley, P.; Bourgeat-Lami, E.; Moskvin, E.; Thomas, M.; Grigoriev, S.; Dyadkin, V.

    2008-01-01

    Neutron Spin Echo (NSE) spectroscopy uses polarized neutrons and accordingly polarization analysis is an intrinsic feature of NSE. However, the multifaceted dynamics of antiferromagnets and helimagnets require more than the classical NSE set-up. Here we present the feasibility test and first results of a new and powerful technique: Polarimetric NSE, obtained by combining the wide angle NSE spectrometer SPAN, developed at HMI with the zero-field polarimeter Cryopad developed at ILL

  15. Fatores associados à procura de serviços de saúde entre escolares brasileiros: uma análise da Pesquisa Nacional de Saúde do Escolar (PeNSE), 2012

    OpenAIRE

    Oliveira, Max Moura de; Andrade, Silvânia Suely Caribé de Araújo; Campos, Maryane Oliveira; Malta, Deborah Carvalho

    2015-01-01

    Os objetivos foram descrever a procura por serviços/profissionais de saúde por escolares brasileiros e identificar fatores associados. Foram analisados dados da Pesquisa Nacional de Saúde do Escolar 2012; estimadas as prevalências e seus respectivos intervalos de 95% de confiança (IC95%) do uso de serviços de saúde entre os escolares. Foi feita regressão logística ajustada por idade. Metade dos estudantes buscaram serviços de saúde; a procura foi maior no sexo feminino; os fatores associados ...

  16. Expression and Effects of High-Mobility Group Box 1 in Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Xiaoao Pang

    2014-05-01

    Full Text Available We investigated the significance of high- mobility group box1 (HMGB1 and T-cell-mediated immunity and prognostic value in cervical cancer. HMGB1, forkhead/winged helix transcription factor p3 (Foxp3, IL-2, and IL-10 protein expression was analyzed in 100 cervical tissue samples including cervical cancer, cervical intraepithelial neoplasia (CIN, and healthy control samples using immunohistochemistry. Serum squamous cell carcinoma antigen (SCC-Ag was immunoradiometrically measured in 32 serum samples from 37 cases of squamous cervical cancer. HMGB1 and SCC-Ag were then correlated to clinicopathological characteristics. HMGB1 expression tends to increase as cervical cancer progresses and it was found to be significantly correlated to FIGO stage and lymph node metastasis. These findings suggest that HMGB1 may be a useful prognostic indicator of cervical carcinoma. In addition, there were significant positive relationships between HMGB1 and FOXP3 or IL-10 expression (both p < 0.05. In contrast, HMGB1 and IL-2 expression was negatively correlated (p < 0.05. HMGB1 expression may activate Tregs or facilitate Th2 polarization to promote immune evasion of cervical cancer. Elevated HMGB1 protein in cervical carcinoma samples was associated with a high recurrence of HPV infection in univariate analysis (p < 0.05. HMGB1 expression and levels of SCC-Ag were directly correlated in SCC (p < 0.05. Thus, HMGB1 may be a useful biomarker for patient prognosis and cervical cancer prediction and treatment.

  17. Effect of different ways of intraoperative lymph node dissection on prognosis of patients with thoracic mid-upper esophageal carcinoma

    Directory of Open Access Journals (Sweden)

    Zhi Huang

    2016-02-01

    Full Text Available Objective: To analyze the effect of different ways of intraoperative lymph node dissection on prognosis of patients with thoracic mid-upper esophageal carcinoma. Methods: 106 cases of patients with thoracic mid-upper esophageal carcinoma were selected and according to different ways of lymph node dissection, divided into three-field group who received three-field lymph node dissection and two-field group who received two-field lymph node dissection, then serum lactate dehydrogenase (LDH, nitric oxide (NO, nitric oxide synthase (NOS and carcinoembryonic antigen (CEA as well as soluble interleukin-2 receptor (SIL- 2R, keratinized protein fragment 19 (Cyfra21-1 and squamous cell carcinoma antigen (SCC levels of two groups were compared, and postoperative follow-up was carried out to record disease-free survival rate and overall survival rate. Results: In three-field group, postoperative average serum LDH levels of patients with thoracic upper esophageal carcinoma and thoracic mid esophageal carcinoma were lower than LDH values of corresponding patients in two-field group (P<0.05; postoperative serum NO value of three-field group was higher than that of two-field group, and NOS, CEA and SIL-2R values were lower than those of two-field group (P<0.05; postoperative serum Cyfra21-1 and SCC values of three-field group were lower than those of two-field group (P<0.05; postoperative disease-free survival rate during the followup period of three-field group was higher than that of two-field group, and overall survival rate at corresponding points in time was also higher than that of two-field group (P<0.05. Conclusion: After patients with thoracic mid-upper esophageal carcinoma receive three-field lymph node dissection, levels of serum indexes with poor prognosis and tumor markers were optimized, long-term disease-free survival rate and overall survival rate are improved. It has positive clinical significance.

  18. Prognostic Value of Pretreatment Carcinoembryonic Antigen After Definitive Radiotherapy With or Without Concurrent Chemotherapy for Squamous Cell Carcinoma of the Uterine Cervix

    International Nuclear Information System (INIS)

    Huang, Eng-Yen; Hsu, Hsuan-Chih; Sun, Li-Min; Chanchien, Chan-Chao; Lin, Hao; Chen, Hui-Chun; Tseng, Chih-Wen; Ou, Yu-Che; Chang, Hung-Yao; Fang, Fu-Min; Huang, Yu-Jie; Wang, Chang-Yu; Lu, Hsien-Ming; Tsai, Ching-Chou

    2011-01-01

    Purpose: To evaluate whether pretreatment carcinoembryonic antigen (CEA) levels have a prognostic role in patients after definitive radiotherapy for squamous cell carcinoma (SCC) of the uterine cervix. Methods and Materials: A retrospective study of 550 patients was performed. The SCC antigen (SCC-Ag) and CEA levels were regarded as elevated when they were ≥2 and ≥5 ng/mL, respectively. A total of 208 patients underwent concurrent chemoradiotherapy (CCRT). The Kaplan-Meier method was used to calculate the distant metastasis (DM), local failure (LF), disease-free survival (DFS), and overall survival (OS) rates. Multivariate analysis was performed using the Cox proportional hazards model. The hazard ratio (HR) with 95% confidence interval (CI) was evaluated for the risk of a poor prognosis. Results: Compared with the patients with normal CEA/SCC-Ag levels, CEA levels ≥10 ng/mL but without elevated SCC-Ag levels was an independent factor for LF (HR, 51.81; 95% CI, 11.51–233.23; p < .001), DM (HR, 6.04; 95% CI, 1.58–23.01; p = .008), DFS (HR, 10.17; 95% CI, 3.18–32.56; p < .001), and OS (HR, 5.75; 95% CI, 1.82–18.18; p = .003) after RT alone. However, no significant role for CEA was noted in patients with SCC-Ag levels ≥2 ng/mL. In patients undergoing CCRT, a CEA level ≥10 ng/mL was an independent factor for LF (HR, 2.50; 95% CI, 1.01–6.21; p = .047), DM (HR, 3.41; 95% CI, 1.56–7.46; p = .002), DFS (HR, 2.73; 95% CI, 1.39–5.36; p = .003), and OS (HR, 3.93; 95% CI 1.99–7.75; p < .001). A SCC-Ag level of ≥40 ng/mL was another prognostic factor for DM, DFS, and OS in patients undergoing not only CCRT, but also RT alone. The 5-year OS rate for CCRT patients with CEA <10 ng/mL and ≥10 ng/mL was 75.3% and 35.8%, respectively (p < .001). CCRT was an independent factor for better OS (HR, 0.69; 95% CI, 0.50–0.97; p = .034). Conclusion: Pretreatment CEA levels in patients with SCC of the uterine cervix provide complementary information for

  19. Nursing Student Self-efficacy in Psychomotor Skills: Findings From a Validation, Longitudinal, and Correlational Study.

    Science.gov (United States)

    Bulfone, Giampiera; Fida, Roberta; Ghezzi, Valerio; Macale, Loreana; Sili, Alessandro; Alvaro, Rosaria; Palese, Alvisa

    Student perceptions of self-efficacy (SE) prevent stress and burnout and improve engagement in nursing education, thus increasing learning outcomes. The study aims were to (1) validate a scale measuring nursing SE in psychomotor skills (NSE-PS), (2) describe changes in NSE-PS over time, and (3) explore NSE-PS correlations with burnout and engagement. A total of 1117 nursing students participated. A significant increase in the NSE-PS scores over the years has emerged; in addition, all NSE-PS dimensions were correlated negatively with burnout and positively with engagement.

  20. Long noncoding RNA TUG1 is a diagnostic factor in lung adenocarcinoma and suppresses apoptosis via epigenetic silencing of BAX.

    Science.gov (United States)

    Liu, Huan; Zhou, Guizhi; Fu, Xin; Cui, Haiyan; Pu, Guangrui; Xiao, Yao; Sun, Wei; Dong, Xinhua; Zhang, Libin; Cao, Sijia; Li, Guiqin; Wu, Xiaowei; Yang, Xu

    2017-11-24

    Lung cancer is one of the leading causes of cancer-related mortality, and responds badly to existing treatment. Thus, it is of urgent need to identify novel diagnostic markers and therapeutic targets. Increasing evidences have indicated that long non-coding RNAs (lncRNAs) play an important role in initiation and progression of lung cancer. However, the role of lncRNA Taurine upregulated 1 (TUG1) in lung adenocarcinoma (LAD) progression is not well known. In this study, we determined the diagnostic value of TUG1 in LAD patients, and further uncovered the underlying functional mechanism. Our results showed that TUG1 was significantly upregulated in LAD cells and serum samples. Receiver operator characteristic (ROC) analysis suggested a relatively higher area under the curve (AUC) of TUG1 (0.756) contrast to cyfra21-1 (0.619). In addition, high TUG1 level was associated with enhanced tumor size, degree of differentiation, lymph node metastases, distant metastasis and TNM stage. Cell functional assays showed that knockdown of TUG1 suppressed LAD cell viability and promoted cell apoptosis. We then sought to reveal the underlying regulatory mechanism, and the pro-apoptotic protein BAX was then identified as the downstream target of TUG1. Gain and loss functional assays showed that inhibition of BAX reversed the induced apoptosis by TUG1 knockdown. Finally, RNA immunoprecipitation and Chromatin immunoprecipitation revealed that TUG1 suppressed BAX expression through physically interacting with EZH2. In conclusion, lncRNA TUG1 is a promising diagnostic marker for LAD patients and suppression of TUG1 levels could be a future direction to promote the prognosis of LAD patients.

  1. Evaluation of Tumor Markers and Their Impact on Prognosis in Gallbladder, Bile Duct and Cholangiocellular Carcinomas - A Pilot Study.

    Science.gov (United States)

    Liska, Vaclav; Treska, Vladislav; Skalicky, Tomas; Fichtl, Jakub; Bruha, Jan; Vycital, Ondrej; Topolcan, Ondrej; Palek, Richard; Rosendorf, Jachym; Polivka, Jiri; Holubec, Lubos

    2017-04-01

    The behavior of tumor markers in biliary tract malignancies is not well-known and has been scarcely studied. Such markers could play important roles in diagnostic and prognostic schemes as well as in decision-making about the best treatment strategies. This study analyzed the preoperative serum levels of conventional tumor markers (AFP, CEA, CA 19-9, CA 72-4), proliferative marker thymidine kinase (TK) and cytokeratins (TPA, TPS and CYFRA 21.1) in patients with gallbladder carcinoma, bile duct carcinoma (Klatskin) and cholangiocellular carcinoma, in relation to the patient prognosis. The study aimed in finding the role of tumor markers in not properly investigated diseases, where their importance is often marginalized. The study included 43 patients, who underwent either radical surgical procedure (n=21) or explorative laparotomy without any surgical treatment (n=22) for gallbladder carcinoma, bile duct carcinoma (Klatskin tumor) and cholangiocellular carcinoma (24, 8 and 11 patients, respectively) between 2003 and 2010 at our Department. The association of serum tumor markers and patients' prognosis were assessed for the entire cohort and for each cancer type and also with regard to treatment (radical surgery versus explorative laparotomy). Overall survival (OS) and disease-free interval (DFI) were estimated by the Kaplan-Meier method and statistically evaluated using the LogRank test. DFI was computed only in the subgroup of patients treated by radical surgery. The statistical analysis of tumor markers revealed TK as a poor prognostic factor for shorter DFI (HR=3.5, 95%CI=0.6-21.3, ptumor markers for assessment of prognosis (OS or DFI) in patients with gallbladder carcinoma, bile duct carcinoma, and cholangiocellular carcinoma. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  2. Expression Profile of Three Splicing Factors in Pleural Cells Based on the Underlying Etiology and Its Clinical Values in Patients with Pleural Effusion

    Directory of Open Access Journals (Sweden)

    A-Lum Han

    2018-02-01

    Full Text Available Splicing factors (SFs are involved in oncogenesis or immune modulation, the common underlying processes giving rise to pleural effusion (PE. The expression profiles of three SFs (HNRNPA1, SRSF1, and SRSF3 and their clinical values have never been assessed in PE. The three SFs (in pellets of PE and conventional tumor markers were analyzed using PE samples in patients with PE (N = 336. The sum of higher–molecular weight (Mw forms of HNRNPA1 (Sum-HMws-HNRNPA1 and SRSF1 (Sum-HMws-SRSF1 and SRSF3 levels were upregulated in malignant PE (MPE compared to benign PE (BPE; they were highest in cytology-positive MPE, followed by tuberculous PE and parapneumonic PE. Meanwhile, the lowest-Mw HNRNPA1 (LMw-HNRNPA1 and SRSF1 (LMw-SRSF1 levels were not upregulated in MPE. Sum-HMws-HNRNPA1, Sum-HMws-SRSF1, and SRSF3, but neither LMw-HNRNPA1 nor LMw-SRSF1, showed positive correlations with cancer cell percentages in MPE. The detection accuracy for MPE was high in the order of carcinoembryonic antigen (CEA, 85%, Sum-HMws-HNRNPA1 (76%, Sum-HMws-SRSF1 (68%, SRSF3, cytokeratin-19 fragments (CYFRA 21-1, LMw-HNRNPA1, and LMw-SRSF1. Sum-HMws-HNRNPA1 detected more than half of the MPE cases that were undetected by cytology and CEA. Sum-HMws-HNRNPA1, but not other SFs or conventional tumor markers, showed an association with longer overall survival among patients with MPE receiving chemotherapy. Our results demonstrated different levels of the three SFs with their Mw-specific profiles depending on the etiology of PE. We suggest that Sum-HMws-HNRNPA1 is a supplementary diagnostic marker for MPE and a favorable prognostic indicator for patients with MPE receiving chemotherapy.

  3. Effect of perioperative application of L-asrginine combined with intacted protein compound preparations on postoperative antitumor immunity and tumor load in patients with gastric cancer

    Directory of Open Access Journals (Sweden)

    Xiu-Lan Jiang

    2016-10-01

    Full Text Available Objective: To analyze the effect of perioperative application of L-arginine combined with intacted protein compound preparations on postoperative antitumor immunity and tumor load in patients with gastric cancer. Methods: A total of 68 patients with gastric cancer received radical operation, and according to different perioperative nutrition intervention, they were divided into control group (normal glucose saline enteral nutrition and observation group (L-arginine combined with intacted protein compound preparations enteral nutrition by half. Postoperative short-term antitumor immune cell levels and serum levels of illness-related indexes, nutrition and inflammation indexes of two groups were detected, patients were followed up for 3 years and the gastric stump MRI changes were observed. Results: Venous blood CD4+ T lymphocyte level and CD4+ /CD8+ ratio of observation group 3 months after treatment were higher than those of control group while CD8+ T lymphocyte and Treg cell levels were lower than those of control group; serum Pentraxin-3, CYFRA21-1, TTF-1 and HE4 levels were lower than those of control group; ALB, PA and IL-2 levels were higher than those of control group while IL-6 and IL-10 levels were lower than those of control group (P<0.05. Gastric stump MRI images 3 years after operation were significantly different between two groups. Conclusions: Perioperative application of L-arginine combined with intacted protein compound preparations can optimize postoperative immune and nutritional state in patients with gastric cancer, and it also has positive effect on reducing the incidence of long-term gastric stump carcinoma and other aspects.

  4. Elevated levels of the complement activation product C4d in bronchial fluids for the diagnosis of lung cancer.

    Directory of Open Access Journals (Sweden)

    Daniel Ajona

    Full Text Available Molecular markers in bronchial fluids may contribute to the diagnosis of lung cancer. We previously observed a significant increase of C4d-containing complement degradation fragments in bronchoalveolar lavage (BAL supernatants from lung cancer patients in a cohort of 50 cases and 22 controls (CUN cohort. The present study was designed to determine the diagnostic performance of these complement fragments (hereinafter jointly referred as C4d in bronchial fluids. C4d levels were determined in BAL supernatants from two independent cohorts: the CU cohort (25 cases and 26 controls and the HUVR cohort (60 cases and 98 controls. A series of spontaneous sputum samples from 68 patients with lung cancer and 10 controls was also used (LCCCIO cohort. Total protein content, complement C4, complement C5a, and CYFRA 21-1 were also measured in all cohorts. C4d levels were significantly increased in BAL samples from lung cancer patients. The area under the ROC curve was 0.82 (95%CI = 0.71-0.94 and 0.67 (95%CI = 0.58-0.76 for the CU and HUVR cohorts, respectively. In addition, unlike the other markers, C4d levels in BAL samples were highly consistent across the CUN, CU and HUVR cohorts. Interestingly, C4d test markedly increased the sensitivity of bronchoscopy in the two cohorts in which cytological data were available (CUN and HUVR cohorts. Finally, in the LCCCIO cohort, C4d levels were higher in sputum supernatants from patients with lung cancer (area under the ROC curve: 0.7; 95%CI = 0.56-0.83. In conclusion, C4d is consistently elevated in bronchial fluids from lung cancer patients and may be used to improve the diagnosis of the disease.

  5. Sarcopenia is a novel poor prognostic factor in male patients with pathological Stage I non-small cell lung cancer.

    Science.gov (United States)

    Tsukioka, Takuma; Nishiyama, Noritoshi; Izumi, Nobuhiro; Mizuguchi, Shinjiro; Komatsu, Hiroaki; Okada, Satoshi; Toda, Michihito; Hara, Kantaro; Ito, Ryuichi; Shibata, Toshihiko

    2017-04-01

    Sarcopenia is the progressive loss of muscle mass and strength, and has a risk of adverse outcomes such as disability, poor quality of life and death. As prognosis depends not only on disease aggressiveness, but also on a patient's physical condition, sarcopenia can predict survival in patients with various cancer types. However, its effects on postoperative prognosis in patients with localized non-small cell lung cancers (NSCLC) have never been reported. We retrospectively investigated 215 male patients with pathological Stage I NSCLC. L3 muscle index is defined as the cross-section area of muscle at the third lumbar vertebra level, normalized for height, and is a clinical measurement of sarcopenia. We then investigated the effect of preoperative sarcopenia on their postoperative prognosis. Our 215 subjects included 30 patients with sarcopenia. Sarcopenia was significantly associated with body mass index, nutritional condition, serum CYFRA 21-1 level and pathological stage, but not with preoperative respiratory function or performance status. Frequency of postoperative complications, length of postoperative hospital stay, thoracic drainage period or causes of death were not correlated with the presence of sarcopenia. The sarcopenia group had a significantly shorter median overall survival (32 months) than the no-sarcopenia group. Sarcopenia might not affect short-term outcomes in patients with early-stage lung cancer. Sarcopenia was a predictor of poor prognosis in male patients with Stage I NSCLC. As sarcopenic patients with NSCLC patients are at risk for significantly worse outcomes, their treatments require careful planning, even for those with Stage I disease. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Evaluation of a Serum Lung Cancer Biomarker Panel.

    Science.gov (United States)

    Mazzone, Peter J; Wang, Xiao-Feng; Han, Xiaozhen; Choi, Humberto; Seeley, Meredith; Scherer, Richard; Doseeva, Victoria

    2018-01-01

    A panel of 3 serum proteins and 1 autoantibody has been developed to assist with the detection of lung cancer. We aimed to validate the accuracy of the biomarker panel in an independent test set and explore the impact of adding a fourth serum protein to the panel, as well as the impact of combining molecular and clinical variables. The training set of serum samples was purchased from commercially available biorepositories. The testing set was from a biorepository at the Cleveland Clinic. All lung cancer and control subjects were >50 years old and had smoked a minimum of 20 pack-years. A panel of biomarkers including CEA (carcinoembryonic antigen), CYFRA21-1 (cytokeratin-19 fragment 21-1), CA125 (carbohydrate antigen 125), HGF (hepatocyte growth factor), and NY-ESO-1 (New York esophageal cancer-1 antibody) was measured using immunoassay techniques. The multiple of the median method, multivariate logistic regression, and random forest modeling was used to analyze the results. The training set consisted of 604 patient samples (268 with lung cancer and 336 controls) and the testing set of 400 patient samples (155 with lung cancer and 245 controls). With a threshold established from the training set, the sensitivity and specificity of both the 4- and 5-biomarker panels on the testing set was 49% and 96%, respectively. Models built on the testing set using only clinical variables had an area under the receiver operating characteristic curve of 0.68, using the biomarker panel 0.81 and by combining clinical and biomarker variables 0.86. This study validates the accuracy of a panel of proteins and an autoantibody in a population relevant to lung cancer detection and suggests a benefit to combining clinical features with the biomarker results.

  7. Risk stratification of patients with advanced squamous cell carcinoma of cervix treated by radiotherapy alone

    International Nuclear Information System (INIS)

    Hong, J.-H.; Tsai, C.-S.; Lai, C.-H.; Chang, T.-C.; Wang, C.-C.; Chou, H.-H.; Lee, Steve P.; Lee, C.-C.; Tang, Simon G.; Hsueh Swei

    2005-01-01

    Purpose: To identify prognostic factors for local and distant relapse and perform risk stratification for patients with advanced cervical cancer treated with radiotherapy (RT) alone. Methods and Materials: A total of 1031 patients with Stage IB-IVA squamous cell carcinoma of the cervix treated with full-course RT but without any chemotherapy were included for analysis. Of these, 311 patients with nonbulky Stage IB-IIA disease were designated the reference group and the other 720 patients were the study group. The associations of stage, squamous cell carcinoma antigen (SCC-ag) level, hemoglobin level, age, cell differentiation, and pelvic lymph node status with treatment failure were evaluated. The independent prognostic factors were identified by multivariate analysis. The study group was further stratified into subgroups using combinations of these risk factors. Results: In the study group, independent risk factors for local relapse were advanced stage and age 2, and positive pelvic lymph nodes. The 5-year distant relapse-free survival rate was 83% for patients with bulky Stage IB-IIA and IIB disease, SCC-ag level 2, and positive lymph nodes. Conclusion: The risk of treatment failure in advanced-stage cervical cancer patients treated by RT alone can be more precisely predicted by risk stratification. A certain subgroup of patients had better control than the others. The benefit of treating these relatively low-risk patients with additional treatment such as concurrent chemotherapy should be further evaluated in prospective studies or meta-analyses

  8. Neutron spin echo and high resolution inelastic spectroscopy

    International Nuclear Information System (INIS)

    Mezei, F.; Hungarian Academy of Sciences, Budapest. Central Research Inst. for Physics)

    1982-01-01

    The principles of neutrons spin echo (NSE) technique are considered. It is shown that the basis of NSE principle is a single step measurement of the change of the neutron velocity in the scattering process. The backscattering soectroscopy and the NSE techniques are compared. The NSF spectrometer is described. It is shown that 0.5 MeV energy resolution achieved in the NSE experiment is about 40 times superior to those achieved by the other techniques. The NSE technique has the unique feature that provides high resolution in neutron energy change independently of the monochromatization of the beam. The NSE instrument not only covers a wider dynamic range on a pulsed source that on a continuous one, but also collects data more efficiently

  9. Bose gas with two- and three-particle interaction: evolution of soliton-like bubbles

    International Nuclear Information System (INIS)

    Barashenkov, I.V.; Kholmurodov, Kh.T.

    1988-01-01

    Solutions of the non-linear Schroedinger equation (NSE) for the Bose gas with two- and three-particle interaction are considered. Problems of soliton-like bubble existence, stability and evolution of the moving soliton are studied. It is shown that at D=2.3 for low-amplitude waves propagating at the transonic velocity the NSE is reduced to a two- and three-dimensional Kadomtsev-Petviashvili (KP) equation and the NSE bubble soliton transfers to the KP one

  10. Serial measurement of neuron specific enolase improves prognostication in cardiac arrest patients treated with hypothermia: A prospective study

    Directory of Open Access Journals (Sweden)

    Storm Christian

    2012-01-01

    Full Text Available Abstract Background Neuron specific enolase (NSE has repeatedly been evaluated for neurological prognostication in patients after cardiac arrest. However, it is unclear whether current guidelines for NSE cutoff levels also apply to cardiac arrest patients treated with hypothermia. Thus, we investigated the prognostic significance of absolute NSE levels and NSE kinetics in cardiac arrest patients treated with hypothermia. Methods In a prospective study of 35 patients resuscitated from cardiac arrest, NSE was measured daily for four days following admission. Outcome was assessed at ICU discharge using the CPC score. All patients received hypothermia treatment for 24 hours at 33°C with a surface cooling device according to current guidelines. Results The cutoff for absolute NSE levels in patients with unfavourable outcome (CPC 3-5 72 hours after cardiac arrest was 57 μg/l with an area under the curve (AUC of 0.82 (sensitivity 47%, specificity 100%. The cutoff level for NSE kinetics in patients with unfavourable outcome (CPC 3-5 was an absolute increase of 7.9 μg/l (AUC 0.78, sensitivity 63%, specificity 100% and a relative increase of 33.1% (AUC 0.803, sensitivity 67%, specificity 100% at 48 hours compared to admission. Conclusion In cardiac arrest patients treated with hypothermia, prognostication of unfavourable outcome by NSE kinetics between admission and 48 hours after resuscitation may be superior to prognostication by absolute NSE levels.

  11. The Cloning and Characterization of the Enolase2 Gene of Gekko japonicus and Its Polyclonal Antibody Preparation

    Directory of Open Access Journals (Sweden)

    Mei Liu

    2013-04-01

    Full Text Available The enolase2 gene is usually expressed in mature neurons and also named neuron specific enolase (NSE. In the present study, we first obtained the NSE gene cDNA sequence by using the RACE method based on the expressed sequence tag (EST fragment from the cDNA library of Gekko japonicus and identified one transcript of about 2.2 kb in central nervous system of Gekko japonicus by Northern blotting. The open reading frame of NSE is 1305 bp, which encodes a 435 amino-acid protein. We further investigated the multi-tissue expression pattern of NSE by RT-PCR and found that the expression of NSE mRNA was very high in brain, spinal cord and low in heart, while it was not detectable in other tissues. The real-time quantitative PCR was used to investigate the time-dependent change in the expression of the NSE mRNA level after gecko spinal cord transection and found it significantly increased at one day, reaching its highest level three days post-injury and then decreasing at the seventh day of the experiment. The recombinant plasmid of pET-32a-NSE was constructed and induced to express His fused NSE protein. The purified NSE protein was used to immunize rabbits to generate polyclonal antisera. The titer of the antiserum was more than 1:65536 determined by ELISA. Western blotting showed that the prepared antibody could specifically recognize the recombinant and endogenous NSE protein. The result of immunohistochemistry revealed that positive signals were present in neurons of the brain and the spinal cord. This study provided the tools of cDNA and polyclonal antibody for studying NSE function in Gekko japonicus.

  12. Metabolic syndrome and its components with neuron-specific enolase: a cross-sectional study in large health check-up population in China.

    Science.gov (United States)

    Wang, Shu-Yi; Zha, Xiao-Juan; Zhu, Xin-Ying; Li, Wen-Bo; Ma, Jun; Wu, Ze-Wei; Wu, Huan; Jiang, Ming-Fei; Wen, Yu-Feng

    2018-04-10

    This study was aimed at investigating the relationship between neuron-specific enolase (NSE) and components of metabolic syndrome (MS). Cross-sectional study. Chinese health check-up population. 40 684 health check-up people were enrolled in this study from year 2014 to 2016. OR and coefficient for MS. The percentage of abnormal NSE and MS was 26.85% and 8.85%, respectively. There were significant differences in sex, body mass index, drinking habit, triglycerides (TGs), high-density lipoprotein cholesterol (HDL-C), blood pressure and MS between low-NSE and high-NSE groups. In logistic regression analysis, elevated NSE was present in MS, higher body mass index, hypertriglyceridaemia, hypertension and low-HDL groups. Stepwise linear analysis showed a negative correlation between NSE and fasting blood glucose (FBG) (<6.0 mmol/L), and a positive correlation between NSE and TGs (<20 mmol/L), systolic blood pressure (75-200 mm Hg), HDL-C (0.75-2.50 mmol/L), diastolic blood pressure (<70 mm Hg) and FBG (6.00-20.00 mmol/L). Furthermore, MS was positively correlated with NSE within the range of 2.00-7.50 ng/mL, but had a negative correlation with NSE within the range of 7.50-23.00 ng/mL. There are associations between NSE with MS and its components. The result suggests that NSE may be a potential predictor of MS. Further research could be conducted in discussing the potential mechanism involved. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  13. The relationship between neuron-specific enolase and prognosis of patients with acute traumatic brain injury

    Directory of Open Access Journals (Sweden)

    Yun-yang LIU

    2015-03-01

    Full Text Available Objective To investigate the relationship between neuron-specific enolase (NSE levels in serum and cerebrospinal fluid (CSF of patients with acute traumatic brain injury (TBI and the prognosis of TBI patients.  Methods A total of 89 patients with acute TBI were divided into light, medium, heavy and severe TBI groups based on admission Glasgow Coma Scale (GCS score. Serum NSE expression levels were detected in all cases and NSE levels in CSF were detected in 18 cases within 12 h after TBI. The expression levels of serum NSE in 20 normal people, except cases of lung disease and nervous system damage, were detected as a control group. Results Compared with the control group, serum NSE expression levels of patients in each TBI group were elevated (P < 0.05, for all, and the NSE levels in severe and heavy TBI groups were higher than that in medium and light groups (P < 0.05, for all. The serum NSE expression levels of patients with cerebral contusion were higher than that of patients with diffuse axonal injury (DAI, P = 0.025, subdural hematoma (P = 0.031 and epidural hematoma (P = 0.021. Serum NSE expression levels were negatively correlated with GCS score (rs = - 0.327, P = 0.024 and Glasgow Outcome Scale (GOS score (rs = - 0.252, P = 0.049. The NSE expression levels of CSF in severe and heavy TBI patients were higher than that of serum (P = 0.039, 0.031.  Conclusions NSE expression changes can be evaluated as an auxiliary indicator in reflecting the degree of acute TBI, typing diagnosis and prognostic evaluation, and NSE levels of CSF is more sensitive than that of serum. DOI: 10.3969/j.issn.1672-6731.2015.03.013

  14. Art as an Avenue to Science Literacy: Teaching Nanotechnology through Stained Glass

    Science.gov (United States)

    Duncan, Kimberly A.; Johnson, Chris; McElhinny, Kyle; Ng, Steve; Cadwell, Katie D.; Zenner Petersen, Greta M.; Johnson, Angela; Horoszewski, Dana; Gentry, Ken; Lisensky, George; Crone, Wendy C.

    2010-01-01

    Nanoscale science and engineering (NSE) and nanotechnology are emerging fields that have captured the attention of scientists and engineers, as well as mainstream media. However, the general public is relatively unaware of NSE, and schools (both K-12 and undergraduate institutions) rarely include nanotechnology as part of their curriculum. To help…

  15. Grænser forudsætter, at man vil bruge magt

    DEFF Research Database (Denmark)

    Østergaard, Uffe

    2016-01-01

    Historisk set. Fra Hadrians mur til de lyseblå gendarmer ved den dansk-tyske grænse: en historie om magt.......Historisk set. Fra Hadrians mur til de lyseblå gendarmer ved den dansk-tyske grænse: en historie om magt....

  16. During Threaded Discussions Are Non-Native English Speakers Always at a Disadvantage?

    Science.gov (United States)

    Shafer Willner, Lynn

    2014-01-01

    When participating in threaded discussions, under what conditions might non¬native speakers of English (NNSE) be at a comparative disadvantage to their classmates who are native speakers of English (NSE)? This study compares the threaded discussion perspectives of closely-matched NNSE and NSE adult students having different levels of threaded…

  17. The neutron spin-echo spectrometer: a new high resolution technique in neutron scattering

    International Nuclear Information System (INIS)

    Nicholson, L.K.

    1981-01-01

    The neutron spin-echo (NSE) spectrometer provides the highest energy resolution available in neutron scattering experiments. The article describes the principles behind the first NSE spectrometer (at the Institute Laue-Langevin, Grenoble, France) and, as an example of one of its applications, some recent results on polymer chain dynamics are presented. (author)

  18. Protein S100 as outcome predictor after out-of-hospital cardiac arrest and targeted temperature management at 33 °C and 36 °C

    DEFF Research Database (Denmark)

    Stammet, Pascal; Dankiewicz, Josef; Nielsen, Niklas

    2017-01-01

    -specific enolase (NSE) did not further improve the AUC. CONCLUSIONS: The allocated target temperature did not affect S100 to a clinically relevant degree. High S100 values are predictive of poor outcome but do not add value to present prognostication models with or without NSE. S100 measured at 24 h and afterward...

  19. Neuron-Specific Enolase as a Predictor of Death or Poor Neurological Outcome After Out-of-Hospital Cardiac Arrest and Targeted Temperature Management at 33°C and 36°C

    DEFF Research Database (Denmark)

    Stammet, Pascal; Collignon, Olivier; Hassager, Christian

    2015-01-01

    levels were assessed in blood samples obtained 24, 48, and 72 h after return of spontaneous circulation. The primary outcome was neurological outcome at 6 months using the cerebral performance category score. RESULTS: NSE was a robust predictor of neurological outcome in a baseline variable......-adjusted model, and target temperature did not significantly affect NSE values. Median NSE values were 18 ng/ml versus 35 ng/ml, 15 ng/ml versus 61 ng/ml, and 12 ng/ml versus 54 ng/ml for good versus poor outcome at 24, 48, and 72 h, respectively (p ... with areas under the receiver-operating curve of 0.85 and 0.86, respectively. High NSE cutoff values with false positive rates ≤5% and tight 95% confidence intervals were able to reliably predict outcome. CONCLUSIONS: High, serial NSE values are strong predictors of poor outcome after OHCA. Targeted...

  20. The role of nuclear science and engineering in the twenty first century

    International Nuclear Information System (INIS)

    Kimura, Itsuro

    1998-01-01

    For the sustainable development of the world and the welfare of mankind in the 21st century, nuclear energy and radiation including particle and photon beams must play an important role, although there is a large negative side in them. As in the 20th century, the activities of both research and education in Nuclear Science and Engineering (NSE) should be positively promoted and enhanced for safer nuclear energy and wider utilization of radiation and various beams and for the diminishment of their negative side. In this paper, a brief history of NSE in the 20th century and its implications are given first. As an example, the NSE activity in the 100 years' history of Kyoto University is shown. And then the 40 years' development of NSE in Japan is briefly introduced. Finally, presented is the way that the research and education in NSE in the 21st century should be. (author)

  1. Clinical and radiological characteristics of central pulmonary adenocarcinoma: a comparison with central squamous cell carcinoma and small cell lung cancer and the impact on treatment response

    Directory of Open Access Journals (Sweden)

    Wang Z

    2018-05-01

    Full Text Available Zhe Wang,1,2 Minghuan Li,2 Yong Huang,3 Li Ma,3 Hui Zhu,2 Li Kong,2 Jinming Yu2 1School of Medicine, Shandong University, Jinan, Shandong, China; 2Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong, China; 3Department of Radiology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong, China Purpose: The proportion of central pulmonary adenocarcinoma (ADC in central-type lung cancer has been gradually increasing due to the overall increasing incidence of pulmonary ADC. But the clinical and radiological characteristics of central ADCs remain unclear. In this study, we compared the clinical and radiological characteristics of central ADCs with those of small cell lung cancers (SCLCs and squamous cell carcinomas (SQCCs and investigated the impact of these characteristics on patients’ treatment response. Patients and methods: The medical records of 302 consecutive patients with central lung cancer from July 2014 to September 2016 were retrospectively reviewed. There were 99 patients with ADC, 95 with SQCC and 108 with SCLC. Computed tomography images were interpreted by two radiologists. Treatment response was determined by Response Evaluation Criteria In Solid Tumors 1.1. Results: Univariate analyses found that younger age, female sex, no history of smoking, higher levels of carcinoembryonic antigen (CEA, contralateral hilum lymphadenopathy, contralateral lung metastasis, pleural nodules and pleural metastasis to the interlobular fissure were significantly correlated with central ADC. Multivariate logistic regression analyses revealed that compared with central SQCC, female sex, younger age, no history of smoking, higher levels of CEA and contralateral hilum lymphadenopathy were the significantly independent indicators of central pulmonary ADC. Furthermore, compared with central SCLC, younger age, higher levels of CEA and cytokeratin 19 fragment (Cyfra21-1, lower

  2. Clinical value of Pro-GRP and T lymphocyte subpopulation for the assessment of immune functions of lung cancer patients after DC-CIK biological therapy.

    Science.gov (United States)

    He, Lijie; Wang, Jing; Chang, Dandan; Lv, Dandan; Li, Haina; Zhang, Heping

    2018-02-01

    The present study investigated the aptness of assessing the levels of progastrin-releasing peptide (Pro-GRP) in addition to the T lymphocyte subpopulation in lung cancer patients prior to and after therapy for determining immune function. A total of 45 patients with lung cancer were recruited and stratified in to a non-small cell lung cancer (NSCLC) and an SCLC group. Prior to and after treatment by combined biological therapy comprising chemotherapy or chemoradiotherapy followed by three cycles of retransformation of autologous dendritic cells-cytokine-induced killer cells (DC-CIK), the peripheral blood was assessed for populations of CD3 + , CD4 + , CD8 + and regulatory T cells (Treg) by flow cytometry, and for the levels of pro-GRP, carcinoembryonic antigen, neuron-specific enolase and Cyfra 21-1. The results revealed that in NSCLC patients, CD8 + T lymphocytes and Treg populations were decreased, and that CD3 + and CD4 + T lymphocytes as well as the CD4 + /CD8 + ratio were increased after therapy; in SCLC patients, CD3 + , CD4 + and CD8 + T lymphocytes were increased, while Treg cells were decreased after treatment compared with those at baseline. In each group, Pro-GRP was decreased compared with that prior to treatment, and in the SCLC group only, an obvious negative correlation was identified between Pro-GRP and the T lymphocyte subpopulation. Furthermore, a significant correlation between Pro-GRP and Tregs was identified in each group. In conclusion, the present study revealed that the immune function of the patients was improved after biological therapy. The results suggested a significant correlation between Pro-GRP and the T lymphocyte subpopulation in SCLC patients. Detection of Pro-GRP may assist the early clinical diagnosis of SCLC and may also be used to assess the immune regulatory function of patients along with the T lymphocyte subpopulation. Biological therapy with retransformed autologous DC-CIK was indicated to enhance the specific elimination

  3. Relationship between serum carcinoembryonic antigen level and epidermal growth factor receptor mutations with the influence on the prognosis of non-small-cell lung cancer patients

    Directory of Open Access Journals (Sweden)

    Cai ZX

    2016-06-01

    Full Text Available Zuxun Cai Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou City, People’s Republic of China Objective: To investigate the relationship between serum carcinoembryonic antigen (CEA level and epidermal growth factor receptor (EGFR gene mutations in non-small-cell lung cancer (NSCLC patients and to analyze the influence of CEA level on postoperative survival time in lung cancer patients. Methods: A total of 296 patients who were treated in Thoracic Surgery Department of Henan Provincial Chest Hospital from September 2011 to September 2013 were recruited. The level of tumor markers, such as CEA, was determined before the surgery, and EGFR gene mutations were detected after surgery. Thereby, the relationship between tumor makers, including CEA, and EGFR mutation and its influence on prognosis could be investigated. Results: Among 296 patients, the positive rate of EGFR gene mutation was 37.84% (112/296; the mutation occurred more frequently in nonsmokers, adenocarcinoma patients, women, and patients aged <60 years (P<0.05. Both tumor markers and chemosensitivity indicators were related to the profile of EGFR mutations. Elevated squamous cell carcinoma and Cyfra21-1 as well as positively expressed ERCC1 were more common in patients with wild-type EGFR (P<0.05, whereas increased CEA level was observed more frequently in patients with EGFR gene mutation (P=0.012. The positive rate of EGFR gene mutations was higher as the serum CEA level increased, that is, the positive rate in patients with serum CEA level <5, 5–20, and >20 µg/L was 39.81%, 45.32%, and 65.47%, respectively (P=0.004. Logistic regression analysis showed that CEA level was an independent factor in predicting EGFR gene mutations, and serum CEA level was also an independent factor in affecting the prognosis of NSCLC patients, as the overall 2-year survival rate was 73.86% in elevated CEA group and 86.43% in normal group (P<0.01. Conclusion: The prognosis of

  4. Telomerase Activity, Cytokeratin 20 and Cytokeratin 19 in Urine Cells of Bladder Cancer Patients

    International Nuclear Information System (INIS)

    Morsi, M.I.; Youssef, A.I.; El-Sedafi, A.S.; Ghazal, A.A.; Zaher, E.R.; Hassouna, M.E.

    2006-01-01

    Aim of the Study: This work aims to search for markers suitable for the screening of bladder cancer, which should be specific, sensitive, reproducible, non-invasive and at acceptable cost. Patients and Methods: The study included 50 patients diagnosed as bladder cancer (35 TCC, 15 SCC) of different stages and grades, 30 patients with various urothelial diseases, besides 20 apparently healthy subjects of matched age and sex to the malignant group. A random midstream urine sample was collected in a sterile container for the determination of telomerase by RT-PCR, keratin 19 by ELSA CYFRA 21-1 IRMA kit, keratin 20 by RT-PCR and immunohistochemical staining, and urine cytology. Results: For all parameters (telomerase, K19, K20 and cytology) the malignant group was significantly different from both the benign and the control groups. None of the four studied parameters was correlated to the stage of the disease, and when it comes to grade, only KI9 showed a significant positive correlation with grade both in TCC and SCe. When ROC curves for all parameters were compared, K 19 had the largest area under the curve, and then comes K20 . o Conclusion: K 19 may be used as a biological marker for the diagnosis of bladder cancer. K 19 could not be used for differential diagnosis of different types of bladder cancer, meanwhile it could be a marker for differentiation that decreases in less differentiated tumors. As a tumor marker, K20 reflects inability to differentiate tumor type or grade in TCC, while in SCC of the bladder it is correlated with the grade. As a method, RT-PCR is superior to immunostaining for the detection of bladder cancer, meanwhile K20 immunohistochemistry ([HC) results were much better than urine cytology as a bladder cancer screening test. haematuria and inflammation reduced the specificity of telomerase assay, which reduced its validity as a tumor marker of bladder cancer. K 19 and K20 are the best candidates as screening tests for the diagnosis of bladder

  5. The prognostic value of serum neuron-specific enolase in traumatic brain injury: systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Feng Cheng

    Full Text Available BACKGROUND: Several studies have suggested that neuron-specific enolase (NSE in serum may be a biomarker of traumatic brain injury. However, whether serum NSE levels correlate with outcomes remains unclear. The purpose of this review was to evaluate the prognostic value of serum NSE protein after traumatic brain injury. METHODS: PubMed and Embase were searched for relevant studies published up to October 2013. Full-text publications on the relationship of NSE to TBI were included if the studies concerned patients with closed head injury, NSE levels in serum after injury, and Glasgow Outcome Scale (GOS or Extended GOS (GOSE scores or mortality. Study design, inclusion criteria, assay, blood sample collection time, NSE cutoff, sensitivity and specificity of NSE for mortality prediction (if sufficient information was provided to calculate these values, and main outcomes were recorded. RESULTS: Sixteen studies were eligible for the current meta-analysis. In the six studies comparing NSE concentrations between TBI patients who died and those who survived, NSE concentrations correlated with mortality (M.D. 0.28, 95% confidence interval (CI, 0.21 to 0.34; I2 55%. In the eight studies evaluating GOS or GOSE, patients with unfavorable outcomes had significantly higher NSE concentrations than those with favorable outcomes (M.D. 0.24, 95% CI, 0.17 to 0.31; I2 64%. From the studies providing sufficient data, the pooled sensitivity and specificity for mortality were 0.79 and 0.50, and 0.72 and 0.66 for unfavorable neurological prognosis, respectively. The areas under the SROC curve (AUC of NSE concentrations were 0.73 (95% CI, 0.66-0.80 for unfavorable outcome and 0.76 (95% CI, 0.62-0.90 for mortality. CONCLUSIONS: Mortality and unfavorable outcome were significantly associated with greater NSE concentrations. In addition, NSE has moderate discriminatory ability to predict mortality and neurological outcome in TBI patients. The optimal discrimination cutoff

  6. A SWAT model validation of nested-scale contemporaneous stream flow, suspended sediment and nutrients from a multiple-land-use watershed of the central USA.

    Science.gov (United States)

    Zeiger, Sean J; Hubbart, Jason A

    2016-12-01

    There is an ongoing need to validate the accuracy of predictive model simulated pollutant yields, particularly from multiple-land-use (i.e. forested, agricultural, and urban) watersheds. However, there are seldom sufficient observed data sets available that supply requisite spatial and temporal resolution and coupled multi-parameter constituents for rigorous model performance assessment. Four years of hydroclimate and water quality data were used to validate SWAT model estimates of monthly stream flow, suspended sediment, total phosphorus, nitrate, nitrite, ammonium, and total inorganic nitrogen from 5 nested-scale gauging sites located in a multiple-land-use watershed of the central USA. The uncalibrated SWAT model satisfactorily simulated monthly stream flow with Nash-Sutcliffe efficiency (NSE) values ranging from 0.50 near the headwaters, to 0.75 near the watershed outlet. However, the uncalibrated model did not accurately simulate monthly sediment, total phosphorus, nitrate, nitrite, ammonium, and total inorganic nitrogen with NSE valuesSWAT model to multiple gauging sites within the watershed improved estimates of monthly stream flow (NSE=0.83), sediment (NSE=0.78), total phosphorus (NSE=0.81), nitrate (NSE=0.90), and total inorganic nitrogen (NSE=0.86). However, NSE values were model performance decreased for sediment, nitrate, and total inorganic nitrogen during the validation period with NSE valuesSWAT model to multiple gauging sites and provide guidance to SWAT model (or similar models) users wishing to improve model performance at multiple scales. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Characterization of projective general linear groups

    Directory of Open Access Journals (Sweden)

    Alireza Khalili Asboei

    2016-03-01

    Full Text Available Let $G$ be a finite group and $pi_{e}(G$ be the set of element orders of $G $. Let $k in pi_{e}(G$ and $s_{k}$ be the number of elements of order $k $ in $G$. Set nse($G$:=${ s_{k} | k in pi_{e}(G}$. In this paper, it is proved if $|G|=|$ PGL$_{2}(q|$, where $q$ is odd prime power and nse$(G= $nse$($PGL$_{2}(q$, then $G cong $PGL$_

  8. Neutron optics using transverse field neutron spin echo method

    International Nuclear Information System (INIS)

    Achiwa, Norio; Hino, Masahiro; Yamauchi, Yoshihiro; Takakura, Hiroyuki; Tasaki, Seiji; Akiyoshi, Tsunekazu; Ebisawa, Toru.

    1993-01-01

    A neutron spin echo (NSE) spectrometer with perpendicular magnetic field to the neutron scattering plane, using an iron yoke type electro-magnet has been developed. A combination of cold neutron guider, supermirror neutron polarizer of double reflection type and supermirror neutron analyser was adopted for the spectrometer. The first application of the NSE spectrometer to neutron optics by passing Larmor precessing neutrons through gas, solid and liquid materials of several different lengths which are inserted in one of the precession field have been examined. Preliminary NSE spectra of this sample geometry are discussed. (author)

  9. Gender and Socioeconomic Status of Children: Pre-Service Teachers' Expectations

    OpenAIRE

    del Río, M. Francisca; Balladares, Jaime

    2010-01-01

    Se puso a prueba empírica el efecto del género y nivel socioeconómico (NSE) de los alumnos en las expectativas de los profesores en formación. Participaron 108 estudiantes de pedagogía, los que leyeron una historia acerca de un estudiante hipotético que presentaba problemas conductuales y de rendimiento. Se manipuló el sexo y NSE del estudiante, creando 4 condiciones. Los participantes percibieron que los estudiantes de NSE bajo tenían peores características personales y un futuro académico m...

  10. Efecto del nivel socioeconómico en el control inhibitorio durante la edad preescolar

    OpenAIRE

    Lozano Gutiérrez,Asucena; Ostrosky,Feggy

    2012-01-01

    Recientemente se ha señalado que el nivel socioeconómico (NSE) juega un papel importante en el logro académico y en el funcionamiento de distintos sistemas cognitivos. El lenguaje y las funciones ejecutivas son los procesos donde se encuentran más diferencias significativas en función del NSE, debido a que son funciones que tardan en desarrollarse y por ellos están sujetas a los efectos adversos asociados al NSE. El objetivo de este estudio fue identificar las diferencias en el desempeño en t...

  11. License - GenLibi | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...nse Update History of This Database Site Policy | Contact Us License - GenLibi | LSDB Archive ...

  12. Current nanoscience and nanoengineering at the Center for ...

    Indian Academy of Sciences (India)

    The Center for Nanoscale Science and Engineering (CeNSE) at the University of ... molecular electronics, nanotube FETs), nanotem-plates for electronics and gas sensors ... This paper provides glimpses of this research and future directions.

  13. Single versus Serial Measurements of Neuron-Specific Enolase and Prediction of Poor Neurological Outcome in Persistently Unconscious Patients after Out-Of-Hospital Cardiac Arrest - A TTM-Trial Substudy

    DEFF Research Database (Denmark)

    Wiberg, Sebastian; Hassager, Christian; Stammet, Pascal

    2017-01-01

    were included from sites participating in the TTM-trial biobank sub study. NSE was measured at 24, 48 and 72 hours after ROSC and follow-up was concluded after 180 days. The primary end point was poor neurological function or death defined by a cerebral performance category score (CPC-score) of 3 to 5...... of the biomarker neuron-specific enolase (NSE) in combination with other predictors of outcome in patients admitted after out-of-hospital cardiac arrest (OHCA). This study sought to investigate the ability of NSE to predict poor outcome in patients remaining unconscious at day three after OHCA. In addition....... RESULTS: A total of 685 (73%) patients participated in the study. At day three after OHCA 63 (9%) patients had died and 473 (69%) patients were not awake. In these patients, a single NSE measurement at 48 hours predicted poor outcome with an area under the receiver operating characteristics curve (AUC...

  14. Disease: H01919 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01919 Proud syndrome Proud syndrome is a syndromic X-linked mental retardation, c...nse mutations in the homeobox domain cause malformation syndromes such as Proud syndrome. Developmental diso

  15. Actitud de Marcha según Nivel Socioeconómico en Adultos Mayores Autovalentes

    Directory of Open Access Journals (Sweden)

    Paul Medina González

    Full Text Available RESUMEN La marcha es fundamental para la funcionalidad de adultos mayores (AM, además su expresión actitudinal se relaciona con diferentes estados emocionales, los cuales son condicionados por el nivel socioeconómico (NSE. El propósito de esta investigación fue evaluar el comportamiento de atributos actitudinales de marcha según el NSE en AM autovalentes. Participaron este estudio 71 AM autovalentes de la comunidad de Talca-Chile, categorizados según el NSE a los cuales se les aplicó el instrumento denominado análisis de forma y esfuerzo de la marcha previo caracterización de confiabilidad. Los resultados indican que el estilo de marcha no es diferente según el NSE en AM autovalentes; sin embargo, los perfiles cualificados presentan variaciones en los anclajes las que son dependientes del género.

  16. Challenges in neutron spin echo spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Pappas, C., E-mail: c.pappas@tudelft.n [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); Faculty of Applied Sciences, Delft University of Technology, Mekelweg 15, 2629 JB Delft (Netherlands); Lelievre-Berna, E.; Falus, P.; Farago, B. [Institut Laue Langevin, 6 rue Jules Horowitz, 38042 Grenoble (France); Bentley, P. [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); Institut Laue Langevin, 6 rue Jules Horowitz, 38042 Grenoble (France); Moskvin, E. [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); PNPI, 188300 Gatchina, Leningrad District (Russian Federation); Krist, Th. [Helmholtz-Zentrum Berlin for Materials and Energy, Glienicker Str. 100, 14109 Berlin (Germany); Grigoriev, S. [PNPI, 188300 Gatchina, Leningrad District (Russian Federation)

    2009-09-01

    With the new brilliant neutron sources and the developments of novel optical elements, neutron spin echo (NSE) spectroscopy evolves to tackle new problems and scientific fields. The new developments pave the way to complex experimental set-ups such as the intensity modulated variant of NSE (IMNSE), a powerful technique which was introduced some 20 years ago but found limited use up to now. With the new compact supermirror or He{sup 3} polarizers IMNSE becomes attractive for a broad range of applications in magnetism, soft matter and biology. A novel development along this line is the polarimetric NSE technique, which combines IMNSE and the zero-field polarimeter Cryopad to access components of the scattered polarization that are transverse to the incoming polarization. Polarimetric NSE is the method of choice for studying chiral fluctuations, as illustrated by new results on the reference helimagnet MnSi.

  17. Neutron spin-echo spectroscopy for diffusion in crystalline solids

    International Nuclear Information System (INIS)

    Kaisermayr, M.; Rennhofer, M.; Vogl, G.; Pappas, C.; Longeville, S.

    2002-01-01

    Neutron spin-echo spectroscopy (NSE) offers unprecedented opportunities in the investigation of diffusion in crystalline systems due to its outstanding energy resolution. NSE not only enables measurements at lower diffusivities than the established techniques of neutron spectroscopy, but it also gives a very immediate access to the different time scales involved in the diffusion process. This is demonstrated in detail on the example of the binary alloy NiGa where the Ni atoms hop between regular sites on the Ni sublattice and anti-sites on the Ga sublattice. Experiments on two different NSE instruments are compared to measurements using neutron backscattering spectroscopy. The potential of NSE for the investigation of jump diffusion and experimental requirements are discussed

  18. Three-dimensional solutions in media with spatial dependence of nonlinear refractive index

    International Nuclear Information System (INIS)

    Kovachev, L.M.; Kaymakanova, N.I.; Dakova, D.Y.; Pavlov, L.I.; Donev, S.G.; Pavlov, R.L.

    2004-01-01

    We investigate a nonparaxial vector generalization of the scalar 3D+1 Nonlinear Schrodinger Equation (NSE). Exact analytical 3D+1 soliton solutions are obtained for the first time in media of spatial dependence of the nonlinear refractive index

  19. Self-focusing of whistler waves

    Science.gov (United States)

    Karpman, V. I.; Kaufman, R. N.; Shagalov, A. G.

    1992-01-01

    The theory of axially symmetric self-focusing of whistler waves, based on the full system of Maxwell equations, is developed. The plasma is described by the magnetohydrodynamic equations including the ponderomotive force from RF field. The nonlinear Schrodinger equations (NSE) for arbitrary azimuthal modes of whistler waves are derived. It is shown that they differ from the NSE for a scalar field; this is connected with an intrinsic angular momentum due to the rotating polarization of whistlers. It is shown that the self-focusing, as described by the NSE, differs in its final stage from the results following the full set of Maxwell equations. The latter gives defocusing after sufficient narrowing of the initial wave beam, due to transformation of the trapped wave into a nontrapped branch which is not contained in the NSE description. The oscillatory character of the defocusing is demonstrated.

  20. Workshop on neutron spin-echo

    Energy Technology Data Exchange (ETDEWEB)

    Aynajian, P.; Habicht, K.; Keller, Th.; Keimer, B.; Mezei, F.; Monkenbusch, M.; Allgaier, J.; Richter, D.; Fetters, L.J.; Muller, K.; Kreiling, S.; Dehnicke, K.; Greiner, A.; Ehlers, G.; Arbe, A.; Colmenero, J.; Richter, D.; Farago, B.; Monkenbusch, M.; Ohl, M.; Butzek, M.; Kozielewski, T.; Monkenbusch, M.; Richter, D.; Pappas, C.; Hillier, A.; Manuel, P.; Cywinski, R.; Bentley, P.; Alba, M.; Mezei, F.; Campbell, I.A.; Zimmermann, U.; Ellis, J.; Jobic, H.; Pickup, R.M.; Pappas, C.; Farago, B.; Cywinski, R.; Haussler, W.; Holderer, O.; Frielinghaus, H.; Byelov, D.; Monkenbusch, M.; Allgaier, J.; Richter, D.; Egger, H.; Hellweg, Th.; Malikova, N.; Cadene, A.; Marry, V.; Dubois, E.; Turq, P.; Gardner, J.S.; Ehlers, G.; Bramwell, St.S.; Grigoriev, S.; Kraan, W.; Rekveldt, T.; Bouwman, W.; Van Dijk, N.; Falus, P.; Vorobiev, A.; Major, J.; Felcher, G.P.; Te-velthuis, S.; Dosch, H.; Vorobiev, A.; Dridi, M.H.; Major, J.; Dosch, H.; Falus, P.; Felcher, G.P.; Te Velthuis, S.G.E.; Bleuel, M.; Broell, M.; Lang, E.; Littrell, K.; Gahler, R.; Lal, J.; Lauter, H.; Toperverg, B.; Lauter, V.; Jernenkov, M.; Stueber, S.; Enderle, M.; Janoschek, M.; Keller, Th.; Klimko, S.; Boeni, P.; Nagao, M.; Yamada, N.; Kawabata, Y.; Seto, H.; Takeda, T.; Yoshizawa, H.; Yoshida, K.; Yamaguchi, T.; Bellissent-Funel, M.C.; Longeville, St

    2005-07-01

    This document gathers the abstracts of most papers presented at the workshop. Neutron spin-echo (NSE) spectroscopy is a well established technique with a growing expert user community, the aim of the meeting was to discuss the latest achievements in neutron spin-echo science and instrumentation. One of the applications presented is the investigation on the microscopic scale of the dynamics of water in montmorillonite clays with Na{sup +} and Cs{sup +} ions in monolayer and bilayer states. The NSE technique has been used in the normal and resonance modes. NSE results show consistently slower dynamics (higher relaxation times) than both time-of-flight technique (TOF) and classical molecular dynamics simulations (MD). In the present TOF and NSE experiments, anisotropy of the water motion in the interlayer is almost impossible to detect, due to the use of powder samples and insufficient resolution. (A.C.)

  1. Sex differences in the vaccine-specific and non-targeted effects of vaccines

    DEFF Research Database (Denmark)

    Flanagan, Katie L; Klein, Sabra L; Skakkebaek, Niels E

    2011-01-01

    Vaccines have non-specific effects (NSE) on subsequent morbidity and mortality from non-vaccine related infectious diseases. Thus NSE refers to any effect that cannot be accounted for by the induction of immunity against the vaccine-targeted disease. These effects are sex-differential, generally...... being more pronounced in females than males. Furthermore, the NSE are substantial causing greater than fifty percent changes in all cause mortality in certain settings, yet have never been systematically tested despite the fact that millions of children receive vaccines each year. As we strive...... to eliminate infectious diseases through vaccination programmes, the relative impact of NSE of vaccines on mortality is likely to increase, raising important questions regarding the future of certain vaccine schedules. A diverse group of scientists met in Copenhagen to discuss non-specific and sex...

  2. SEXUAL ABUSE IN ADOLESCENTS - DATA FROM A ...

    African Journals Online (AJOL)

    abused patients scored higher on depression rating scales than non sexually ... between sexual abuse and depresSIon, suicidal symptoms and alcohol nse is .... psychiatric symptoms, including negative cognitions, suicidal. Not present.

  3. "Kõige selgem õiguslik järjepidevus valitses Eestimaa kubermangus" : ühe baltisaksa ajalookirjutuse paradigma kriitika / Andres Andresen

    Index Scriptorium Estoniae

    Andresen, Andres, 1971-

    2013-01-01

    Õiguslikust järjepidevusest tuleneva õiguspärasuse printsiibi vastandumisest riigivõimu suveräänse seadusandluse printsiibile. Eestimaa kirikukorralduse põhijoontest kuni 1710. aastani ja muudatustest pärast seda.

  4. President Ilves meets with German Chancellor : "The democratic countries are at a decision-making threshold"

    Index Scriptorium Estoniae

    2008-01-01

    President Toomas Hendrik Ilvese kohtumisest Saksamaa liidukantsler Angela Merkeliga, kes saabus Eestisse ühepäevasele ametlikule visiidile. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu

  5. Percepciones sobre disponibilidad de alimentos y autorreporte de ingesta alimentaria en mujeres urbanas costarricenses: Un estudio piloto

    Directory of Open Access Journals (Sweden)

    Traci A. Bekelman

    2016-01-01

    Full Text Available Antecedentes: Se considera que la disponibilidad y consumo de alimentos han contribuido al aumento de obesidad entre las mujeres de bajo nivel socioeconómico (NSE en Latinoamérica. En Costa Rica, pocos estudios han investigado cómo la disponibilidad y consumo varían según NSE. Objetivos: Los objetivos de este estudio en San José, Costa Rica, son (1 evaluar percepciones sobre la disponibilidad de alimentos y (2 describir el consumo de alimentos en mujeres que viven en áreas de alto y bajo NSE. Métodos: En marzo 2013, se reclutaron 30 mujeres no embarazadas, ni dando lactancia materna, de 25 a 50 años, en un cantón de bajo y otro de alto NSE. Las percepciones sobre disponibilidad de alimentos se analizaron de manera cualitativa mediante entrevistas estructuradas a 17 de las 30 mujeres. El consumo de alimentos se analizó de manera cuantitativa mediante recordatorios de 24 horas en las 30 mujeres. Resultados: Muchas mujeres del cantón de bajo NSE reportaron que incluirían más pollo y pescado en su dieta si el costo no fuera una barrera. La ingesta de proteína como proporción del valor energético total fue significativamente mayor en las mujeres del cantón de alto NSE (17% DE=5 vs. 13 DE=3, p = 0.02. La ingesta de proteína de origen animal fue significativamente mayor entre las mujeres del cantón de alto NSE (38 g DE=22 vs. 21 DE=11, p=0.02. Conclusión: La ingesta de proteína fue mayor en las mujeres de alto NSE y puede deberse al costo percibido de los alimentos de origen animal.

  6. Neuron-specific enolase is a useful maker of neuroendocrine origin in pheochromocytoma cell culture

    International Nuclear Information System (INIS)

    Abelin, N.; Dahia, P.L.M.; Martin, R.; Kato, S.; Toledo, S.P.A.

    1994-01-01

    Neuron-specific enolase (NSE) has been used as a marker for neuroendocrine tumors either in immunocytochemical studies or in serum measurements. In this paper NSE levels were determined in cultured pheochromocytoma cells to test whether it is also a useful marker in cell culture of tumors derived from neuroendocrine system. Cultured pheochromocytoma cells came from a primary explant and were grown in RPMI supplemented with 20% fetal calf serum, 100 μg/mL ampicillin and 100 μ/mL streptomycin. NSE was measured in culture medium and cell homogenates. Samples from different pheochromocytoma cultures were analyzed and compared to normal cultured fibroblast cells derived from human skin. NSE was measured by a commercially available radioimmunoassay kit. NSE levels were higher in cell homogenates as compared to those in culture medium, reaching levels as high as 6-fold in the former in TE cell line (26.46 ng/mL and 4.39 ng/mL, respectively). Serial measurements in culture medium from TE cell line evidenced decreasing values in subsequential subcultures (from 9.24 ng/mL during primary explant to 1.7 ng/mL in the tenth subculture). In cultured normal fibroblasts, NSE levels in cultured media were definitely lower than those obtained from pheochromocytoma cultures. These preliminary data suggest that NSE may be a useful marker of neuroendocrine derived tumors, such as pheochromocytoma, in culture. Thus, the simplicity and availability of NSE radioimmunoassay provides an alternative to catecholamine measurement to better characterize pheochromocytoma cell lines in culture, with the advantage of faster result at lower costs. (author). 18 refs, 2 tabs

  7. CRISPR - nye forædlingsteknologier i planter

    DEFF Research Database (Denmark)

    Brinch-Pedersen, Henrik; Holme, Inger

    2018-01-01

    En række nye fremskridt med CRISPR teknikken i spidsen har nu gjort det muligt at begrænse mutationer til de regioner i plantegenomet, man ønsker skal muteres.......En række nye fremskridt med CRISPR teknikken i spidsen har nu gjort det muligt at begrænse mutationer til de regioner i plantegenomet, man ønsker skal muteres....

  8. Differential expression of the neural cell adhesion molecule NCAM 140 in human pituitary tumors

    OpenAIRE

    Aletsee-Ufrecht, M. C.; Langley, O. K.; Gratzl, O.; Gratzl, Manfred

    1990-01-01

    We have analyzed the expression of the intracellular marker protein neuron specific enolase (NSE), synaptophysin (SYN) and of the cell surface marker NCAM (neural cell adhesion molecule) in both normal human hypophysis and in pituitary adenomas in order to explore their potential use as diagnostic tools. All adenomas (4 prolactinomas, 3 growth hormone (GH) producing adenomas and 4 inactive adenomas) showed SYN and NSE immunoreactivity on tissue sections and this was confirmed by immunoblots. ...

  9. Serum Neuron-Specific Enolase, Biogenic Amino-Acids and Neurobehavioral Function in Lead-Exposed Workers from Lead-Acid Battery Manufacturing Process

    OpenAIRE

    K Ravibabu; T Barman; HR Rajmohan

    2015-01-01

    Background: The interaction between serum neuron-specific enolase (NSE), biogenic amino-acids and neurobehavioral function with blood lead levels in workers exposed to lead form lead-acid battery manufacturing process was not studied. Objective: To evaluate serum NSE and biogenic amino-acids (dopamine and serotonin) levels, and neurobehavioral performance among workers exposed to lead from lead-acid storage battery plant, and its relation with blood lead levels (BLLs). Methods: In a c...

  10. Direct observation of the transition from free to constrained single segment motion in entangled polymer melts

    International Nuclear Information System (INIS)

    Monkenbusch, M.; Wischnewski, A.; Willner, L.; Richter, D.

    2004-01-01

    Incoherent neutron-spin-echo spectroscopy (NSE) has been employed to directly determine the time-dependent mean-squared segment displacement 2 > of a polymer chain in the melt covering the transition from free to constraint Rouse relaxation along the virtual tube of the reptation model. The predicted transition of the time dependence of 2 > from 2 >∝t 1/2 to ∝t 1/4 is clearly corroborated by the incoherent NSE results

  11. Evaluation of Composting for Reducing Volume of Solid Waste on Contingency Bases

    Science.gov (United States)

    2012-05-23

    Reduce SW on Contingency Bases, 23 May 2012, E2S2 5 National Def nse Cent rgy and Environment • An excellent soil amendment that adds stable organics...and nutrients to improve the soil • Natural fertilizer and valuable humus that promotes weed and erosion control, protects plant roots...National Def nse Cent rgy and Environment CompTainers HotRot System Rocket® Composter DTE ENVIRO -DRUM EcoPOD Ag-Bag Technology National Defense

  12. Correlative study between neuron-specific enolase and blood sugar level in ischemic stroke patients

    OpenAIRE

    Pandey, Aparna; Saxena, Kiran; Verma, Meena; Bharosay, Anuradha

    2011-01-01

    Background: A study to investigate the level of the neurobiochemical marker, Neuron-Specific Enolase (NSE), at the time of admission and its correlation with the blood sugar level in ischemic stroke patients. Patients and Methods: We investigated 90 patients with complete stroke who were admitted to the Stroke Unit of the Department of Neurology at Sri Aurobindo Institute of Medical Sciences. NSE was measured with commercially available quantitative ′sandwich′ enzyme-linked immunosorbent assa...

  13. Plasma antioxidant capacity, sexual and thyroid hormones levels, sperm quantity and quality parameters in stressed male rats received nano-particle of selenium

    Directory of Open Access Journals (Sweden)

    M Rezaeian-Tabrizi

    2017-01-01

    Full Text Available Objective: To evaluate the effects of nano-particle of selenium (nSe on plasma antioxidant capacity, sexual and thyroid hormones and spermatogenesis in male rats exposed to oxidative stress.Methods: Forty rats were randomly divided into four treatments with ten replicates. Treatment groups were: C, the control group received normal saline as gavage and injection (i.p.; OS, received tert-butyl hydroperoxide (0.2 mmol/kg body weight for inducing oxidative stress; nSe, received nSe (0.3 mg/kg body weight as gavage, and OS+nSe, received tert-butyl hydroperoxide and nSe. All groups were treated for 28 d and administrations were done each 48 h.Results: Oxidative stress decreased and gavage of nSe to stressed rats increased the antioxidant capacity and activities (P0.05 between rats exposed to oxidative stress and those in the control group for sperm quantity and quality. Gavage of nSe to stressed rats had no effect (P>0.05 on the sperm parameters, except increased viability and progressive percentages.Conclusions: Nano-particle of Selenium administration in stressed rats could ameliorate the negative effects of oxidative stress on the antioxidant capacity and activities, but not on the quantity and quality parameters of sperm.

  14. Combined Scintigraphy and Tumor Marker Analysis Predicts Unfavorable Histopathology of Neuroblastic Tumors with High Accuracy.

    Directory of Open Access Journals (Sweden)

    Wolfgang Peter Fendler

    Full Text Available Our aim was to improve the prediction of unfavorable histopathology (UH in neuroblastic tumors through combined imaging and biochemical parameters.123I-MIBG SPECT and MRI was performed before surgical resection or biopsy in 47 consecutive pediatric patients with neuroblastic tumor. Semi-quantitative tumor-to-liver count-rate ratio (TLCRR, MRI tumor size and margins, urine catecholamine and NSE blood levels of neuron specific enolase (NSE were recorded. Accuracy of single and combined variables for prediction of UH was tested by ROC analysis with Bonferroni correction.34 of 47 patients had UH based on the International Neuroblastoma Pathology Classification (INPC. TLCRR and serum NSE both predicted UH with moderate accuracy. Optimal cut-off for TLCRR was 2.0, resulting in 68% sensitivity and 100% specificity (AUC-ROC 0.86, p < 0.001. Optimal cut-off for NSE was 25.8 ng/ml, resulting in 74% sensitivity and 85% specificity (AUC-ROC 0.81, p = 0.001. Combination of TLCRR/NSE criteria reduced false negative findings from 11/9 to only five, with improved sensitivity and specificity of 85% (AUC-ROC 0.85, p < 0.001.Strong 123I-MIBG uptake and high serum level of NSE were each predictive of UH. Combined analysis of both parameters improved the prediction of UH in patients with neuroblastic tumor. MRI parameters and urine catecholamine levels did not predict UH.

  15. Neuron- specific enolase level in patients with metabolic syndrome and its value forecasting acute stroke

    Directory of Open Access Journals (Sweden)

    Oral Ospanov

    2018-03-01

    Full Text Available Background Patients with metabolic syndrome are at a greater risk of experiencing a cerebrovascular event. Several studies show that patients with metabolic syndrome have asymptomatic ischemic brain injury. In this case, there is a need for rapid determination of asymptomatic brain lesions and prediction of acute stroke. Aims The aim of the study was to determine the neuron-specific enolase (NSE serum level in patients with metabolic syndrome and the value of this level for forecasting acute stroke. Methods The study used the following information to determine metabolic syndrome: waist circumference, total cholesterol, triglycerides, high-density lipoprotein cholesterol, blood pressure, and blood glucose. Doppler sonography mapping of the brachiocephalic trunk was held to determine the percentage of the carotid artery stenosis. To determine asymptomatic ischemic brain injury, the NSE serum marker was measured. Statistical processing of the measurements was performed using the H test and the Mann–Whitney test. The possible link between MS and NSE were determined by logistic regression analysis. Mathematical modeling was performed using logistic regression. Results There are statistically significant differences in NSE concentrations in groups with metabolic syndrome and ischemic stroke patients. This assertion is confirmed by logistic regression analysis, which revealed the existence of a relationship between metabolic syndrome and increased concentration of NSE. Conclusion Patients with metabolic syndrome have an increased concentration of NSE. This indicates the presence of asymptomatic ischemic neuronal damage. A prognostic model for determining the probability that patients with metabolic syndrome will have an acute stroke was developed.

  16. Determination of serum neuron specific enolase and glutathion S transferases levels in patients with acute cerebral infarction and its clinical significance

    International Nuclear Information System (INIS)

    Guo Jianyi; Lu Tianhe; Bao Yanmei

    2002-01-01

    Objective: To evaluate the variation of serum neuron specific enolase (NSE) and glutathion S transferases (GST) levels in patients with cerebral infarction and its clinical significance. Methods: The serum levels of NSE in cerebral infarction patients were determined with immunoradiometric assay (IRMA), and the serum level of GST were determined by enzyme immuno sandwich assay (ELISA). Results: Serum NSE levels linked in patients were significantly higher (p<0.01) and GST serum levels were significantly lower (p < 0.01) within 3 days after onset of disease than those at two weeks and those in the controls. There was a positive correlation between serum NSE levels and neurological deficit scores (p < 0.001) and a negative correlation with serum GST levels (p < 0.05). There was also a close relationship between the serum NSE levels and the volume of infarction (p < 0.001). Conclusion: There was a close relationship between the Serum levels of NSE, GST and clinical features of Patients in the early stage of cerebral infarction

  17. Evaluating the prognosis and degree of brain injury by combined S-100 protein and neuron specific enolase determination

    Institute of Scientific and Technical Information of China (English)

    Xihua Wang; Xinding Zhang

    2006-01-01

    Background:S-100 and neuron specific enolase(NSE)possess the characteristics of specific distribution in brain and relative stable content.Some studies suggest that combined detection of the both is of very importance for evaluating the degree of brain injury.OBJECTIVE: To observe the changes of S-100 protein and NSE levels at different time points after acute brain injury,and evaluate the values of combined detection detection of the both for different injury degrees,pathological changes and prognosis.DESIGN: Case-control observation SETTING: Department of Neurosurgery,Second Affiliated Hospital,Lanzhou University.PARTICIPANTS:Thirty-four inpatients with brain injury,19 males and 15 females,aged 15 to 73 years.who received treatment between September 2005 and May 2006 in the Department of Neurosurgery. Second Affiliated Hospital,Lanzhou University,were recruited.The patients were admitted to hospital at 24 hours after brain injury.After admission,skull CT confirmed that they suffered from brain injury.Following Glasgow coma score(GCS)on admission,the patients were assigned into 3 groups:severe group(GCS 3 to 8 points,n=15).moderate group(GCS 9 to 12 points,n=8)and mild group(GCS 13 to 15 points,n=11).Following Glasgow outcome scale(GOS)at 3 months after brain injury,the patients were assigned into good outcome group (GOS 4 to 5 points,good recovery and moderate disability included,n=19)and poor outcome group(GOS 1 to 3 points,severe disability,vegetative state and death,n=15).Ten subjects who received health examination concurrently were chosen as normal control group,including 6 males and 4 females,aged(45.4±14.3)years.In our laboratory,the normal level of NSE was≤15.2 ng/L,and that of S100 was≤0.105 μg/L.METHODS:①Blood samples of control group were collected when the subjects received health examination Blood samples of patients with brain injury were collected at 24 hours,3,7 and 14 days after injury.According to the instructions of NSE and S-100 kits

  18. Effect of a dual-task net-step exercise on cognitive and gait function in older adults.

    Science.gov (United States)

    Kitazawa, Kazutoshi; Showa, Satoko; Hiraoka, Akira; Fushiki, Yasuhiro; Sakauchi, Humio; Mori, Mitsuru

    2015-01-01

    Participation in generally recommended aerobics or strength exercises may be challenging for older adults. Therefore, it is necessary to consider the types and levels of physical activities suited for them to improve their cognitive and gait function and adherence to exercise programs. This has prompted efforts to identify exercises that require less physical strength and frequency of performance, while still offering cognitive and health benefits. Here, we aimed to assess the effect of a novel dual-task net-step exercise (NSE) performed once a week for 8 consecutive weeks on improvements in cognitive performance and gait function in an older population. In this pretest/posttest experimental case control study, 60 healthy older adults (mean age 76.4 years) were recruited from community-dwelling people and separated randomly into 2 groups: a dual-task NSE group and a control group. The NSE group was asked to walk across a net without stepping on the ropes or being caught in the net. Two computer panel-type cognitive functional assessments, the Touch-M and Touch Panel-Type Dementia Assessment Scale, were administered at baseline and after 8 weeks of intervention to determine the effects of NSE. Improvements in gait function were also evaluated using Timed Up and Go test scores. Mixed-effect models with repeated measures (group × time) (analysis of variance, F test) were used to test the effects of NSE. Adjustments were made for covariates including age and sex (analysis of covariance). The NSE group showed significant improvement in cognitive performance (6.8% change; total Touch-M score 5.4 points; P = .04) and gait performance (11.5% change; Timed Up and Go time -0.98 second; P cognitive and gait performance in healthy older adults. Our results indicate that NSE offers an option for a large segment of the older population who need an easier way to maintain their cognitive health and gait function.

  19. Uncovering a Nuisance Influence of a Phenological Trait of Plants Using a Nonlinear Structural Equation: Application to Days to Heading and Culm Length in Asian Cultivated Rice (Oryza Sativa L.).

    Science.gov (United States)

    Onogi, Akio; Ideta, Osamu; Yoshioka, Takuma; Ebana, Kaworu; Yamasaki, Masanori; Iwata, Hiroyoshi

    2016-01-01

    Phenological traits of plants, such as flowering time, are linked to growth phase transition. Thus, phenological traits often influence other traits through the modification of the duration of growth period. This influence is a nuisance in plant breeding because it hampers genetic evaluation of the influenced traits. Genetic effects on the influenced traits have two components, one that directly affects the traits and one that indirectly affects the traits via the phenological trait. These cannot be distinguished by phenotypic evaluation and ordinary linear regression models. Consequently, if a phenological trait is modified by introgression or editing of the responsible genes, the phenotypes of the influenced traits can change unexpectedly. To uncover the influence of the phenological trait and evaluate the direct genetic effects on the influenced traits, we developed a nonlinear structural equation (NSE) incorporating a nonlinear influence of the phenological trait. We applied the NSE to real data for cultivated rice (Oryza sativa L.): days to heading (DH) as a phenological trait and culm length (CL) as the influenced trait. This showed that CL of the cultivars that showed extremely early heading was shortened by the strong influence of DH. In a simulation study, it was shown that the NSE was able to infer the nonlinear influence and direct genetic effects with reasonable accuracy. However, the NSE failed to infer the linear influence in this study. When no influence was simulated, an ordinary bi-trait linear model (OLM) tended to infer the genetic effects more accurately. In such cases, however, by comparing the NSE and OLM using an information criterion, we could assess whether the nonlinear assumption of the NSE was appropriate for the data analyzed. This study demonstrates the usefulness of the NSE in revealing the phenotypic influence of phenological traits.

  20. No more tears? Maternal involvement during the newborn screening examination.

    LENUS (Irish Health Repository)

    Ganda, Augustine Josie

    2012-01-31

    BACKGROUND: Babies often show signs of discomfort and distress by crying during the neonatal screening examination (NSE). The authors hypothesized that supporting the baby with maternal participation may reduce infant crying during NSE. The objective of this study was to document incidental infant crying during NSE, before and after training residents, on maternal involvement and infant comfort techniques to help. METHODS: A total of 20 NSEs of normal newborn babies by pediatric residents were observed (video-recorded) following informed consent of the doctor and the baby\\'s mother. The examining doctors were then taught how to use maternal participation and developmental care (MPDC) comfort techniques to support the baby during NSE. Mothers were shown how to focus on their baby\\'s needs by supporting the baby\\'s head (preventing atonic neck reflexes) and, if necessary, providing nonnutritive sucking to the baby and an encouraging, repetitive low-tone voices to sooth the baby. A further 14 NSEs on different babies were video-recorded using these techniques. The video recordings were analyzed by independent observers for total length of crying and duration of crying during specific components of the NSE. Mothers in both groups were given a questionnaire to assess their opinions of the NSE. RESULTS: The median length of crying was significantly longer in the pre-MPDC group (93.5 seconds; range 0-198 seconds) compared with the post-MPDC infants (0 seconds; range 0-123 seconds; P = .001). Only 1 of 20 infants in the pre-MPDC did not cry during NSE compared with 8 of 14 babies in the post-MPDC group. CONCLUSION: Newborn infants cry less and mothers were more satisfied with NSEs when shown simple support and comfort techniques for their babies.

  1. Research of the serum level of neuron-specific enolase in children with various types of seizure

    Directory of Open Access Journals (Sweden)

    WANG Chun

    2012-10-01

    Full Text Available Objective To explore the relevance between the level changes of serum neuron-specific enolase (NSE and neuronal damage in various seizure types of children with epilepsy. Methods According to the classification criteria of seizure types formulated by International League Against Epilepsy (ILAE in 1981, 190 children with epilepsy were enrolled including tonic-clonic seizure group (41 cases, tonic seizure group (34 cases, clonic seizure group (22 cases, myoclonic seizure group (12 cases, atonic seizure group (17 cases, absence seizure group (22 cases, simple partial seizure group (21 cases and complex partial seizure group (21 cases, and 64 healthy children were enrolled as control group. The long-range vedio-electroencephalogram (VEEG was operated and the blood samples were collected from these cases within 72 h after their seizures. Results The serum NSE levels of epileptic children were significantly higher than control group (P = 0.000. Among these seizure groups, serum NSE in myoclonic seizure group [(32.42 ± 6.62 ng/ml] was significantly higher than the other types, except for tonic-clonic seizure group (P = 0.062. There was no significant difference among the other types (P > 0.05, for all. According to rank correlation analysis, there was positive corrlation between serum NSE levels and VEEG abnormal intensity (rs = 0.613, P = 0.000. Conclusion The serum NSE were markedly increased in children with epilepsy after seizures, suggesting that a certain degree of neuronal damage may result from seizures; the higher NSE levels were, the more serious neuronal damage caused by epileptiform discharges was. The serum NSE levels in myoclonic seizure group and tonic-clonic seizure group were significantly higher than other seizure types, indicating the two kinds of seizures may result in greater neuronal damage.

  2. Serum neuron specific enolase - a novel indicator for neuropsychiatric systemic lupus erythematosus?

    Science.gov (United States)

    Hawro, T; Bogucki, A; Krupińska-Kun, M; Maurer, M; Woźniacka, A

    2015-12-01

    Neuropsychiatric (NP) lupus, a common manifestation of systemic lupus erythematosus (SLE), is still insufficiently understood, in part, because of the lack of specific biomarkers. Neuron specific enolase (NSE), an important neuronal glycolytic enzyme, shows increased serum levels following acute brain injury, and decreased serum levels in several chronic disorders of the nervous system, including multi infarct dementia, multiple sclerosis and depression. The aim of the study was to evaluate serum NSE levels in SLE patients with and without nervous system involvement, and in healthy controls, and to assess the correlation of NSE serum levels of patients with neuropsychiatric systemic lupus erythematosus (NPSLE) with clinical parameters. The study comprised 47 SLE patients and 28 controls. SLE activity was assessed using the Systemic Lupus Activity Measure (SLAM). A neurologist and a psychiatrist examined all patients. NP involvement was diagnosed according to strict NPSLE criteria proposed by Ainiala and coworkers, as modification to American College of Rheumatology (ACR) nomenclature and case definitions. NSE serum levels were determined by use of an immunoassay. Mean NSE serum concentrations in patients with NPSLE were significantly lower than in non-NPSLE patients (6.3 ± 2.6 µg/L vs. 9.7 ± 3.3 µg/L, p < 0.01) and in controls (8.8 ± 3.3 µg/L, p < 0.05). There were significant negative correlations between NSE serum levels and SLE activity (r = -0.42, p < 0.05) and the number of NPSLE manifestations diagnosed (-0.37; p = 0.001). Decreased serum concentrations of NSE may reflect chronic neuronal damage with declined metabolism of the nervous tissue in patients with NPSLE. © The Author(s) 2015.

  3. Carcinoembryonic antigen (CEA) as tumor marker in lung cancer.

    Science.gov (United States)

    Grunnet, M; Sorensen, J B

    2012-05-01

    The use of CEA as a prognostic and predictive marker in patients with lung cancer is widely debated. The aim of this review was to evaluate the results from studies made on this subject. Using the search words "CEA", "tumor markers in lung cancer", "prognostic significance", "diagnostic significance" and "predictive significance", a search was carried out on PubMed. Exclusion criteria was articles never published in English, articles before 1981 and articles evaluating tumor markers in lung cancer not involving CEA. Initially 217 articles were found, and 34 were left after selecting those relevant for the present study. Four of these included both Non-Small Cell Lung Cancer (NSCLC) and Small Cell Lung Cancer (SCLC) patients, and 31 dealt solely with NSCLC patients. Regarding SCLC no studies showed that serum level of CEA was a prognostic marker for overall survival (OS). The use of CEA serum level as a prognostic marker in NSCLC was investigated in 23 studies and the use of CEA plasma level in two. In 18 (17 serum, 1 plasma) of these studies CEA was found to be a useful prognostic marker for either OS, recurrence after surgery or/and progression free survival (PFS) in NSCLC patients. Interestingly, an overweight of low stage (stage I-II) disease and adenocarcinoma (AC) patients were observed in this group. The remaining 7 studies (6 serum, 1 plasma) contained an overweight of patients with squamous carcinoma (SQ). One study found evidence for that a tumor marker index (TMI), based on preoperative CEA and CYFRA21-1 serum levels, is useful as a prognostic marker for OS in NSCLC. Six studies evaluated the use of CEA as a predictive marker for risk of recurrence and risk of death in NSCLC patients. Four of these studies found, that CEA was useful as a predictive marker for risk of recurrence and risk of death measured over time. No studies found CEA levels useful as a diagnostic marker for lung cancer. With regard to NSCLC the level of CEA measured in tumor tissue in

  4. The Serum Concentrations of Chemokine CXCL12 and Its Specific Receptor CXCR4 in Patients with Esophageal Cancer

    Directory of Open Access Journals (Sweden)

    Marta Łukaszewicz-Zając

    2016-01-01

    Full Text Available Objectives. Recent investigations have suggested that upregulated levels of inflammatory biomarkers, such as chemokines, may be associated with development of many malignancies, including esophageal cancer (EC. Based on our knowledge, this study is the first to assess the serum concentration of chemokine CXCL12 and its specific receptor CXCR4 in the diagnosis of EC patients. Material and Methods. The present study included 79 subjects: 49 patients with EC and 30 healthy volunteers. The serum concentrations of CXCL12 and CXCR4 and classical tumor markers such as carcinoembryonal antigen (CEA and squamous cell cancer antigen (SCC-Ag were measured using immunoenzyme assays, while C-reactive protein (CRP levels were assessed by immunoturbidimetric method. Moreover, diagnostic criteria of all proteins tested and the survival of EC patients were assessed. Results. The serum concentrations of CXCL12 were significantly higher, while those of its receptor CXCR4 were significantly lower in EC patients compared to healthy controls. The diagnostic sensitivity, negative predictive value, and accuracy of CXCR4 were the highest among all analyzed proteins and increased for combined analysis with classical tumor markers and CRP levels. Conclusion. Our findings suggest that serum CXCR4 may improve the diagnosis of EC patients, especially in combination with classical tumor markers.

  5. Correlative study between neuron-specific enolase and blood sugar level in ischemic stroke patients

    Directory of Open Access Journals (Sweden)

    Aparna Pandey

    2011-01-01

    Full Text Available Background: A study to investigate the level of the neurobiochemical marker, Neuron-Specific Enolase (NSE, at the time of admission and its correlation with the blood sugar level in ischemic stroke patients. Patients and Methods: We investigated 90 patients with complete stroke who were admitted to the Stroke Unit of the Department of Neurology at Sri Aurobindo Institute of Medical Sciences. NSE was measured with commercially available quantitative ′sandwich′ enzyme-linked immunosorbent assay kits obtained from R and D Systems. Hyperglycemia was defined as blood glucose concentration ≥ 7 mmol / L, and measured using the glucose oxidase method immediately. Results: Significantly increased NSE and lipid profile levels were found in ischemic stroke patients as compared to the control. Hyperglycemic ischemic stroke patients had increased levels of NSE, lipid profile, and National Institute of Health stroke scale scores (NIHSS score compared to normoglycemic ischemic stroke patients. In addition the serum NSE level of hyperglycemic stroke patients was also positively correlated with the blood sugar level (r = 0.734 P < 0.001. Conclusions: Hyperglycemia predicts an increased risk of poor outcome after ischemic stroke and it is reflected by a significantly increased level of Neuron-Specific Enolase.

  6. Label-free electrochemical immunoassay for neuron specific enolase based on 3D macroporous reduced graphene oxide/polyaniline film.

    Science.gov (United States)

    Zhang, Qi; Li, Xiaoyan; Qian, Chunhua; Dou, Li; Cui, Feng; Chen, Xiaojun

    2018-01-01

    The content of neuron specific enolase (NSE) in serum is considered to be an essential indicator of small cell lung cancer (SCLC). Here, a novel label-free electrochemical immunoassay for the detection of NSE based on the three dimensionally macroporous reduced graphene oxide/polyaniline (3DM rGO/PANI) film has been proposed. The 3DM rGO/PANI film was constructed by electrochemical co-deposition of GO and aniline into the interspaces of a sacrificial silica opal template modified Au slice. During the co-deposition, GO was successfully reduced by aniline and PANI could be deposited on the surfaces of rGO sheets. The ratio of rGO and PANI in the composite was also optimized to achieve the maximum electrochemical performance. The 3DM rGO/PANI composite provided larger specific surface area for the antibody immobilization, exhibited enhanced conductivity for electron transfer, and more important was that PANI acted as the electroactive probe for indicating the NSE concentration. Under the optimal conditions, a linear current response of PANI to NSE concentration was obtained over 0.5 pg mL -1 -10.0 ng mL -1 with a detection limit of 0.1 pg mL -1 . Moreover, the immunosensor showed excellent selectivity, good stability, satisfactory reproducibility and regeneration, and was employed to detect NSE in clinical serum specimens. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. The effect of neck-specific exercise with, or without a behavioral approach, on pain, disability, and self-efficacy in chronic whiplash-associated disorders: a randomized clinical trial.

    Science.gov (United States)

    Ludvigsson, Maria L; Peterson, Gunnel; O'Leary, Shaun; Dedering, Åsa; Peolsson, Anneli

    2015-04-01

    The aim of this study was to compare the effect on self-rated pain, disability, and self-efficacy of 3 interventions for the management of chronic whiplash-associated disorders: physiotherapist-led neck-specific exercise (NSE), physiotherapist-led NSE with the addition of a behavioral approach, or Prescription of Physical Activity (PPA). A total of 216 volunteers with chronic whiplash-associated disorders participated in this randomized, assessor blinded, clinical trial of 3 exercise interventions. Self-rated pain/pain bothersomeness (Visual Analogue Scale), disability (Neck Disability Index), and self-efficacy (Self-Efficacy Scale) were evaluated at baseline and at 3 and 6 months. The proportion of patients reaching substantial reduction in pain bothersomness (at least 50% reduction) was more evident (Pexercise groups at both 3 and 6 months (PSelf-efficacy was only improved in the NSE group without a behavioral approach (P=0.02). However, there were no significant differences in any outcomes between the 2 physiotherapist-led NSE groups. NSE resulted in superior outcomes compared with PPA in this study, but the observed benefits of adding a behavioral approach to the implementation of exercise in this study were inconclusive.

  8. DrSPINE - New approach to data reduction and analysis for neutron spin echo experiments from pulsed and reactor sources

    International Nuclear Information System (INIS)

    Zolnierczuk, P.A.; Ohl, M.; Holderer, O.; Monkenbusch, M.

    2015-01-01

    Neutron spin echo (NSE) method at a pulsed neutron source presents new challenges to the data reduction and analysis as compared to the instruments installed at reactor sources. The main advantage of the pulsed source NSE is the ability to resolve the neutron wavelength and collect neutrons over a wider bandwidth. This allows us to more precisely determine the symmetry phase and measure the data for several Q-values at the same time. Based on the experience gained at the SNS NSE - the first, and to date the only one, NSE instrument installed at a pulsed spallation source, we propose a novel and unified approach to the NSE data processing called DrSPINE. The goals of the DrSPINE project are: -) exploit better symmetry phase determination due to the broader bandwidth at a pulsed source; -) take advantage of larger Q coverage for TOF instruments; -) use objective statistical criteria to get the echo fits right; -) provide robust reduction with report generation; -) incorporate absolute instrument calibration; and -) allow for background subtraction. The software must be able to read the data from various instruments, perform data integrity, consistency and compatibility checks and combine the data from compatible sets, partial scans, etc. We chose to provide a console-based interface with the ability to process macros (scripts) for batch evaluation. And last and not the least, a good software package has to provide adequate documentation. DrSPINE software is currently under development

  9. Longitudinal study on patent citations to academic research articles in nanotechnology (1976-2004)

    International Nuclear Information System (INIS)

    Hu Daning; Chen Hsinchun; Huang Zan; Roco, Mihail C.

    2007-01-01

    Academic nanoscale science and engineering (NSE) research provides a foundation for nanotechnology innovation reflected in patents. About 60% or about 50,000 of the NSE-related patents identified by 'full-text' keyword searching between 1976 and 2004 at the United States Patent and Trademark Office (USPTO) have an average of approximately 18 academic citations. The most cited academic journals, individual researchers, and research articles have been evaluated as sources of technology innovation in the NSE area over the 28-year period. Each of the most influential articles was cited about 90 times on the average, while the most influential author was cited more than 700 times by the NSE-related patents. Thirteen mainstream journals accounted for about 20% of all citations. Science, Nature and Proceedings of the National Academy of Sciences (PNAS) have consistently been the top three most cited journals, with each article being cited three times on average. There is another kind of influential journals, represented by Biosystems and Origin of Life, which have very few articles cited but with exceptionally high frequencies. The number of academic citations per year from ten most cited journals has increased by over 17 times in the interval (1990-1999) as compared to (1976-1989), and again over 3 times in the interval (2000-2004) as compared to (1990-1999). This is an indication of increased used of academic knowledge creation in the NSE-related patents

  10. Serum neuron-specific enolase, biogenic amino-acids and neurobehavioral function in lead-exposed workers from lead-acid battery manufacturing process.

    Science.gov (United States)

    Ravibabu, K; Barman, T; Rajmohan, H R

    2015-01-01

    The interaction between serum neuron-specific enolase (NSE), biogenic amino-acids and neurobehavioral function with blood lead levels in workers exposed to lead form lead-acid battery manufacturing process was not studied. To evaluate serum NSE and biogenic amino-acids (dopamine and serotonin) levels, and neurobehavioral performance among workers exposed to lead from lead-acid storage battery plant, and its relation with blood lead levels (BLLs). In a cross-sectional study, we performed biochemical and neurobehavioral function tests on 146 workers exposed to lead from lead-acid battery manufacturing process. BLLs were assessed by an atomic absorption spectrophotometer. Serum NSE, dopamine and serotonin were measured by ELISA. Neurobehavioral functions were assessed by CDC-recommended tests---simple reaction time (SRT), symbol digit substitution test (SDST), and serial digit learning test (SDLT). There was a significant correlation (r 0.199, pSDLT and SRT had also a significant positive correlation (r 0.238, p<0.01). NSE had a negative correlation (r -0.194, p<0.05) with serotonin level. Multiple linear regression analysis revealed that both SRT and SDST had positive significant associations with BLL. SRT also had a positive significant association with age. Serum NSE cannot be used as a marker for BLL. The only domain of neurobehavioral function tests that is affected by increased BLL in workers of lead-acid battery manufacturing process is that of the "attention and perception" (SDST).

  11. An Improved Nested Sampling Algorithm for Model Selection and Assessment

    Science.gov (United States)

    Zeng, X.; Ye, M.; Wu, J.; WANG, D.

    2017-12-01

    Multimodel strategy is a general approach for treating model structure uncertainty in recent researches. The unknown groundwater system is represented by several plausible conceptual models. Each alternative conceptual model is attached with a weight which represents the possibility of this model. In Bayesian framework, the posterior model weight is computed as the product of model prior weight and marginal likelihood (or termed as model evidence). As a result, estimating marginal likelihoods is crucial for reliable model selection and assessment in multimodel analysis. Nested sampling estimator (NSE) is a new proposed algorithm for marginal likelihood estimation. The implementation of NSE comprises searching the parameters' space from low likelihood area to high likelihood area gradually, and this evolution is finished iteratively via local sampling procedure. Thus, the efficiency of NSE is dominated by the strength of local sampling procedure. Currently, Metropolis-Hasting (M-H) algorithm and its variants are often used for local sampling in NSE. However, M-H is not an efficient sampling algorithm for high-dimensional or complex likelihood function. For improving the performance of NSE, it could be feasible to integrate more efficient and elaborated sampling algorithm - DREAMzs into the local sampling. In addition, in order to overcome the computation burden problem of large quantity of repeating model executions in marginal likelihood estimation, an adaptive sparse grid stochastic collocation method is used to build the surrogates for original groundwater model.

  12. Longitudinal study on patent citations to academic research articles in nanotechnology (1976-2004)

    Energy Technology Data Exchange (ETDEWEB)

    Hu Daning, E-mail: hud@email.arziona.edu; Chen Hsinchun [University of Arizona, Artificial Intelligence Lab, Department of Management Information Systems, Eller College of Management (United States); Huang Zan [The Pennsylvania State University, Department of Supply Chain and Information Systems, Smeal College of Business (United States); Roco, Mihail C. [National Science Foundation (United States)

    2007-08-15

    Academic nanoscale science and engineering (NSE) research provides a foundation for nanotechnology innovation reflected in patents. About 60% or about 50,000 of the NSE-related patents identified by 'full-text' keyword searching between 1976 and 2004 at the United States Patent and Trademark Office (USPTO) have an average of approximately 18 academic citations. The most cited academic journals, individual researchers, and research articles have been evaluated as sources of technology innovation in the NSE area over the 28-year period. Each of the most influential articles was cited about 90 times on the average, while the most influential author was cited more than 700 times by the NSE-related patents. Thirteen mainstream journals accounted for about 20% of all citations. Science, Nature and Proceedings of the National Academy of Sciences (PNAS) have consistently been the top three most cited journals, with each article being cited three times on average. There is another kind of influential journals, represented by Biosystems and Origin of Life, which have very few articles cited but with exceptionally high frequencies. The number of academic citations per year from ten most cited journals has increased by over 17 times in the interval (1990-1999) as compared to (1976-1989), and again over 3 times in the interval (2000-2004) as compared to (1990-1999). This is an indication of increased used of academic knowledge creation in the NSE-related patents.

  13. Non-specific effects of vaccines: plausible and potentially important, but implications uncertain.

    Science.gov (United States)

    Pollard, Andrew J; Finn, Adam; Curtis, Nigel

    2017-11-01

    Non-specific effects (NSE) or heterologous effects of vaccines are proposed to explain observations in some studies that certain vaccines have an impact beyond the direct protection against infection with the specific pathogen for which the vaccines were designed. The importance and implications of such effects remain controversial. There are several known immunological mechanisms which could lead to NSE, since it is widely recognised that the generation of specific immunity is initiated by non-specific innate immune mechanisms that may also have wider effects on adaptive immune function. However, there are no published studies that demonstrate a mechanistic link between such immunological phenomena and clinically relevant NSE in humans. While it is highly plausible that some vaccines do have NSE, their magnitude and duration, and thus importance, remain uncertain. Although the WHO recently concluded that current evidence does not justify changes to immunisation policy, further studies of sufficient size and quality are needed to assess the importance of NSE for all-cause mortality. This could provide insights into vaccine immunobiology with important implications for infant health and survival. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  14. Proton-rich nuclear statistical equilibrium

    International Nuclear Information System (INIS)

    Seitenzahl, I.R.; Timmes, F.X.; Marin-Lafleche, A.; Brown, E.; Magkotsios, G.; Truran, J.

    2008-01-01

    Proton-rich material in a state of nuclear statistical equilibrium (NSE) is one of the least studied regimes of nucleosynthesis. One reason for this is that after hydrogen burning, stellar evolution proceeds at conditions of an equal number of neutrons and protons or at a slight degree of neutron-richness. Proton-rich nucleosynthesis in stars tends to occur only when hydrogen-rich material that accretes onto a white dwarf or a neutron star explodes, or when neutrino interactions in the winds from a nascent proto-neutron star or collapsar disk drive the matter proton-rich prior to or during the nucleosynthesis. In this Letter we solve the NSE equations for a range of proton-rich thermodynamic conditions. We show that cold proton-rich NSE is qualitatively different from neutron-rich NSE. Instead of being dominated by the Fe-peak nuclei with the largest binding energy per nucleon that have a proton-to-nucleon ratio close to the prescribed electron fraction, NSE for proton-rich material near freezeout temperature is mainly composed of 56Ni and free protons. Previous results of nuclear reaction network calculations rely on this nonintuitive high-proton abundance, which this Letter explains. We show how the differences and especially the large fraction of free protons arises from the minimization of the free energy as a result of a delicate competition between the entropy and nuclear binding energy.

  15. The effect of N-stearoylethanolamine on plasma lipid composition in rats with experimental insulin resistance

    Directory of Open Access Journals (Sweden)

    O. V. Onopchenko

    2015-02-01

    Full Text Available A model of insulin resistance (IR, induced by prolonged high fat diet with high content of saturated fats was used to investigate the effect of N-stearoylethanolamine (NSE on the composition of free fatty acids (FFA, plasma lipoprotein spectrum and content of proinflammatory cytokine TNFα in rats. The results of this work showed a rise in the content of monounsaturated fatty acids (18:1 n-9 and a reduction in the level of polyunsaturated fatty acids (20:4 n-6 in plasma of rats with experimental IR. These findings are accompanied by the increased TNFα production and significant changes in plasma lipoprotein profile of rats with the fat overload. Particularly, a decreased high-density lipoprotein (HDL cholesterol level and increased low-density (LDL and very low-density lipoprotein (VLDL cholesterol level were detected. The NSE administration to obese rats with IR restored the content of mono- and polyunsaturated FFA, increased HDL cholesterol content and reduced LDL cholesterol level. In addition, the IR rats treated with NSE showed normalization in the serum TNFα level. Our results showed the restoration of plasma lipid profile under NSE administration in rats with obesity-induced IR. Considering the fact that plasma lipid composition displays the lipid metabolism in general, the NSE actions may play a significant role in the prevention of IR-associated complications.

  16. Weak Form Efficiency of the Nigerian Stock Market: An Empirical Analysis (1984 – 2009

    Directory of Open Access Journals (Sweden)

    Pyemo Afego

    2012-01-01

    Full Text Available This paper examines the weak-form of the efficient markets hypothesis for the Nigerian Stock Exchange (NSE by testing for random walks in the monthly index returns over the period 1984-2009. The results of the non-parametric runs test show that index returns on the NSE display a predictable component, thus suggesting that traders can earn superior returns by employing trading rules. Statistically significant deviations from randomness are also suggestive of sub-optimal allocation of investment capital within the economy. The findings, in general, contradict the weak-form of the efficient markets hypothesis, and a range of policy strategies for improving the allocative capacity and quality of the information environment of the NSE are discussed.

  17. Ansiedade, sexo, nível sócio-econômico e ordem de nascimento Anxiety, sex, socioeconomic status, and birth order

    Directory of Open Access Journals (Sweden)

    Jorge La Rosa

    1998-01-01

    Full Text Available O objetivo do estudo foi verificar o efeito do sexo, nível sócio-econômico (NSE e ordem de nascimento em ansiedade traço-estado. Participaram 437 estudantes do 1° e 2° graus, de ambos os sexos, de níveis sócio-econômicos médio-alto e baixo, primogênitos e não-primogênitos. No que se refere à ansiedade estado (AE, observaram-se efeitos principais de sexo e NSE. As mulheres apresentaram escores mais altos que os homens, e também os sujeitos de NSE baixo com relação aos de NSE médio-alto. Houve interação entre NSE e ordem de nascimento. Os estudantes primogênitos de NSE médio-alto evidenciaram menor AE que os primogênitos e não-primogênitos de NSE baixo. Em outra interação, as mulheres primogênitas de NSE baixo apresentaram maior AE que os homens de NSE médio-alto, primogênitos e não-primogênitos, e, também, que as mulheres primogênitas de NSE médio-alto. Nos resultados de ansiedade-traço, as mulheres obtiveram pontuação mais alta que os homens, e também os sujeitos de NSE baixo com relação aos de NSE médio-alto. Não houve interações. Discutem-se os resultados enfatizando-se a importância do sexo, nível sócio-econômico e ordem de nascimento nos níveis de ansiedade traço-estado.The objective of this study was to verify the effect of sex, socioeconomic status (SES and birth order on state-trait anxiety. The subjects were 437 primary and secondary school students of both sexes, firt-borns and non-first-borns, belonging to both upper-middle and lower socioeconomic levels. Regarding state-anxiety (SA, main effects of the variables sex and SES could be observed. Women presented higher scores than men, and so did the subjects from lower SES in relation to the subjects of upper-middle SES. There was an interaction between SES and birth order. Students who were first-borns of the upper-middle SES showed lower SA than first-borns and non first-borns of lower SES. In another interaction, female first-borns of

  18. International nanotechnology development in 2003: Country, institution, and technology field analysis based on USPTO patent database

    International Nuclear Information System (INIS)

    Huang Zan; Chen Hsinchun; Chen Zhikai; Roco, Mihail C.

    2004-01-01

    Nanoscale science and engineering (NSE) have seen rapid growth and expansion in new areas in recent years. This paper provides an international patent analysis using the U.S. Patent and Trademark Office (USPTO) data searched by keywords of the entire text: title, abstract, claims, and specifications. A fraction of these patents fully satisfy the National Nanotechnology Initiative definition of nanotechnology (which requires exploiting specific phenomena and direct manipulation at the nanoscale), while others only make use of NSE tools and methods of investigation. In previous work we proposed an integrated patent analysis and visualization framework of patent content mapping for the NSE field and of knowledge flow pattern identification until 2002. In this paper, the results are updated for 2003, and the new trends are presented

  19. Early detection of response in small cell bronchogenic carcinoma by changes in serum concentrations of creatine kinase, neuron specific enolase, calcitonin, ACTH, serotonin and gastrin releasing peptide

    DEFF Research Database (Denmark)

    Bork, E; Hansen, M; Urdal, P

    1988-01-01

    Creatine kinase (CK-BB), neuron specific enolase (NSE), ACTH, calcitonin, serotonin and gastrin releasing peptide (GRP) were measured in serum or plasma before and immediately after initiation of treatment in patients with small cell lung cancer (SCC). Pretherapeutic elevated concentrations of CK...... stage patients and 71% in limited stage patients. Frequent initial monitoring of the substances showed an increase in the concentrations of pretherapeutic elevated CK-BB and NSE on day 1 or 2 followed by a sharp decrease within 1 week. These changes were correlated to objective clinical response...... determined within 4-8 weeks. The results indicate that serum CK-BB and NSE are potential markers for SCC at the time of diagnosis and that changes in the concentrations during the first course of cytostatic therapy are promising as biochemical tests for early detection of response to chemotherapy....

  20. International nanotechnology development in 2003: Country, institution, and technology field analysis based on USPTO patent database

    Science.gov (United States)

    Huang, Zan; Chen, Hsinchun; Chen, Zhi-kai; Roco, Mihail C.

    2004-08-01

    Nanoscale science and engineering (NSE) have seen rapid growth and expansion in new areas in recent years. This paper provides an international patent analysis using the U.S. Patent and Trademark Office (USPTO) data searched by keywords of the entire text: title, abstract, claims, and specifications. A fraction of these patents fully satisfy the National Nanotechnology Initiative definition of nanotechnology (which requires exploiting specific phenomena and direct manipulation at the nanoscale), while others only make use of NSE tools and methods of investigation. In previous work we proposed an integrated patent analysis and visualization framework of patent content mapping for the NSE field and of knowledge flow pattern identification until 2002. In this paper, the results are updated for 2003, and the new trends are presented.

  1. Projection of the rotation form Navier-Stokes equation onto the half-staggered grid

    Energy Technology Data Exchange (ETDEWEB)

    Cho, Ji Ryong [Inje University, Kimhae (Korea, Republic of)

    2016-07-15

    A projection method for computing incompressible fluid flow is proposed. For the method, the rotation form Navier-Stokes equation (NSE), for which the velocity and the total pressure are employed, is discretized on the half-staggered, finite difference spatial grid. The total pressure couples the static pressure gradient and the convection of momentum in the continuous NSE while the half-staggered grid provides weak pressure-velocity coupling in discrete space. These two features interact synergistically for the discretized NSE to produce smooth pressure fields without additional numerical artifacts such as the momentum interpolation. The method preserves the kinetic energy at the inviscid limit condition. Numerical solutions of the decaying Taylor vortex, the inviscid Taylor vortex, the sudden expansion channel and the square-prism wake are presented.

  2. Nonlinear Source Emulator

    DEFF Research Database (Denmark)

    Nguyen-Duy, Khiem

    of a proposed NSE system with high dynamic performance. The goal of the work is to achieve a state-of-the art transient time of 10 µs. In order to produce the arbitrary nonlinear curve, the exponential function of a typical diode is used, but the diode can be replaced by other nonlinear curve reference...... of conductive common-mode current produced by the high rate of change of voltage over time (high dv/dt) at the NSE output. v/xvii The contributions of the thesis are based on the development of both units: the low Cio isolated power supply and the high dynamic performance NSE. Both units are investigated......-of-the-art dynamic performance among devices of the same kind. It also offers a complete solution for simulation of nonlinear source systems of different sizes, both in terrestrial and non-terrestrial applications. Key words: Current transformers, dc-dc power converters, hysteresis, parasitic capacitance, system...

  3. Concepciones de tweens chilenos respecto a la publicidad emitida por televisión

    Directory of Open Access Journals (Sweden)

    Carlos del Valle

    2010-01-01

    Full Text Available Esta investigación buscó identificar los usos y significados que escolares de 11 a 12 años de edad (tweens otorgan a la publicidad emitida por televisión. En la etapa cuantitativa se aplicó el 'Cuestionario de hábitos de consumo televisivo y prácticas de uso del dinero' a una muestra de 137 escolares, de los cuales 28 pertenecen al nse alto, 62 al nse medio-alto y 47 al nse bajo. En la etapa cualitativa se aplicó una entrevista en profundidad a 30 escolares de la muestra. Los resultados señalan la existencia de diferencias significativas según género y grado socioeconómico, lo cual permite hipotetizar que estas variables poseen influencia en los usos, opiniones y percepciones que los tweens poseen de la publicidad.

  4. Comparative role of neem seed extract, moringa leaf extract and imidacloprid in the management of wheat aphids in relation to yield losses in Pakistan.

    Science.gov (United States)

    Shah, Farhan Mahmood; Razaq, Muhammad; Ali, Abid; Han, Peng; Chen, Julian

    2017-01-01

    Wheat being staple food of Pakistan is constantly attacked by major wheat aphid species, Schizaphis graminum (R.), Rhopalosiphum padi (L.) and Sitobion avenae (F.). Due to concern on synthetic chemical use in wheat, it is imperative to search for alternative environment- and human- friendly control measures such as botanical pesticides. In the present study, we evaluated the comparative role of neem seed extract (NSE), moringa leaf extract (MLE) and imidacloprid (I) in the management of the aphid as well as the yield losses parameters in late planted wheat fields. Imidacloprid reduced significantly aphids infestation compared to the other treatments, hence resulting in higher yield, particularly when applied with MLE. The percentages of yield increase in I+MLE treated plots over the control were 19.15-81.89% for grains per spike, 5.33-37.62% for thousand grain weight and 27.59-61.12% for yield kg/ha. NSE was the second most effective control measure in suppressing aphid population, but the yield protected by NSE treatment over the control was comparable to that by imidacloprid. Population densities of coccinellids and syrphids in the plots treated with NSE-2 were higher than those treated with imidacloprid in two out of three experiments during 2013-14. Low predator density in imidacloprid-treated plots was attributed to the lower availability of prey aphids. The efficacy of NSE against aphids varied depending on degree of synchronization among the application timing, the activity of aphids, crop variety and environmental conditions. Despite that, we suggested NSE to be a promising alternative botanical insecticide compared to the most commonly recommended imidiacloprid. Further studies should consider the side effects of biopesticides on non-target organisms in order to provide better management practices in the field.

  5. Hydrologic Modeling and Parameter Estimation under Data Scarcity for Java Island, Indonesia

    Science.gov (United States)

    Yanto, M.; Livneh, B.; Rajagopalan, B.; Kasprzyk, J. R.

    2015-12-01

    The Indonesian island of Java is routinely subjected to intense flooding, drought and related natural hazards, resulting in severe social and economic impacts. Although an improved understanding of the island's hydrology would help mitigate these risks, data scarcity issues make the modeling challenging. To this end, we developed a hydrological representation of Java using the Variable Infiltration Capacity (VIC) model, to simulate the hydrologic processes of several watersheds across the island. We measured the model performance using Nash-Sutcliffe Efficiency (NSE) at monthly time step. Data scarcity and quality issues for precipitation and streamflow warranted the application of a quality control procedure to data ensure consistency among watersheds resulting in 7 watersheds. To optimize the model performance, the calibration parameters were estimated using Borg Multi Objective Evolutionary Algorithm (Borg MOEA), which offers efficient searching of the parameter space, adaptive population sizing and local optima escape facility. The result shows that calibration performance is best (NSE ~ 0.6 - 0.9) in the eastern part of the domain and moderate (NSE ~ 0.3 - 0.5) in the western part of the island. The validation results are lower (NSE ~ 0.1 - 0.5) and (NSE ~ 0.1 - 0.4) in the east and west, respectively. We surmise that the presence of outliers and stark differences in the climate between calibration and validation periods in the western watersheds are responsible for low NSE in this region. In addition, we found that approximately 70% of total errors were contributed by less than 20% of total data. The spatial variability of model performance suggests the influence of both topographical and hydroclimatic controls on the hydrological processes. Most watersheds in eastern part perform better in wet season and vice versa for the western part. This modeling framework is one of the first attempts at comprehensively simulating the hydrology in this maritime, tropical

  6. Development of an algorithm for phenotypic screening of carbapenemase-producing Enterobacteriaceae in the routine laboratory.

    Science.gov (United States)

    Robert, Jérôme; Pantel, Alix; Merens, Audrey; Meiller, Elodie; Lavigne, Jean-Philippe; Nicolas-Chanoine, Marie-Hélène

    2017-01-17

    Carbapenemase-producing Enterobacteriaceae (CPE) are difficult to identify among carbapenem non-susceptible Enterobacteriaceae (NSE). We designed phenotypic strategies giving priority to high sensitivity for screening putative CPE before further testing. Presence of carbapenemase-encoding genes in ertapenem NSE (MIC > 0.5 mg/l) consecutively isolated in 80 French laboratories between November 2011 and April 2012 was determined by the Check-MDR-CT103 array method. Using the Mueller-Hinton (MH) disk diffusion method, clinical diameter breakpoints of carbapenems other than ertapenem, piperazicillin+tazobactam, ticarcillin+clavulanate and cefepime as well as diameter cut-offs for these antibiotics and temocillin were evaluated alone or combined to determine their performances (sensitivity, specificity, positive and negative likelihood ratios) for identifying putative CPE among these ertapenem-NSE isolates. To increase the screening specificity, these antibiotics were also tested on cloxacillin-containing MH when carbapenem NSE isolates belonged to species producing chromosomal cephalosporinase (AmpC) but Escherichia coli. Out of the 349 ertapenem NSE, 52 (14.9%) were CPE, including 39 producing OXA-48 group carbapenemase, eight KPC and five MBL. A screening strategy based on the following diameter cut offs, ticarcillin+clavulanate <15 mm, temocillin <15 mm, meropenem or imipenem <22 mm, and cefepime <26 mm, showed 100% sensitivity and 68.1% specificity with the better likelihood ratios combination. The specificity increased when a diameter cut-off <32 mm for imipenem (76.1%) or meropenem (78.8%) further tested on cloxacillin-containing MH was added to the previous strategy for AmpC-producing isolates. The proposed strategies that allowed for increasing the likelihood of CPE among ertapenem-NSE isolates should be considered as a surrogate for carbapenemase production before further CPE confirmatory testing.

  7. Effect of Memantine on Serum Levels of Neuron-Specific Enolase and on the Glasgow Coma Scale in Patients With Moderate Traumatic Brain Injury.

    Science.gov (United States)

    Mokhtari, Majid; Nayeb-Aghaei, Hossein; Kouchek, Mehran; Miri, Mir Mohammad; Goharani, Reza; Amoozandeh, Arash; Akhavan Salamat, Sina; Sistanizad, Mohammad

    2018-01-01

    Traumatic brain injury (TBI) is a major cause of disability and death globally. Despite significant progress in neuromonitoring and neuroprotection, pharmacological interventions have failed to generate favorable results. We examined the effect of memantine on serum levels of neuron-specific enolase (NSE), a marker of neuronal damage, and the Glasgow Coma Scale (GCS) in patients with moderate TBI. Patients were randomly assigned to the control group (who received standard TBI management) and the treatment group (who, alongside their standard management, received enteral memantine 30 mg twice daily for 7 days). Patients' clinical data, GCS, findings of head computed tomography, and serum NSE levels were collected during the study. Forty-one patients were randomized into the control and treatment groups, 19 and 22 patients respectively. Baseline characteristics and serum NSE levels were not significantly different between the 2 groups. The mean serum NSE levels for the memantine and the control groups on day 3 were 7.95 ± 2.86 and 12.33 ± 7.09 ng/mL, respectively (P = .05), and on day 7 were 5.03 ± 3.25 and 10.04 ± 5.72 ng/mL, respectively (P = .003). The mean GCS on day 3 was 12.3 ± 2.0 and 10.9 ± 1.9 in the memantine and control groups, respectively (P = .03). Serum NSE levels and GCS changes were negatively correlated (r = -0.368, P = .02). Patients with moderate TBI who received memantine had significantly reduced serum NSE levels by day 7 and marked improvement in their GCS scores on day 3 of the study. © 2017, The American College of Clinical Pharmacology.

  8. Comment on "What is the entanglement length in a polymer melt?" by M. Pütz, K. Kremer and G. S. Grest

    Science.gov (United States)

    Wischnewski, A.; Richter, D.

    2000-12-01

    In a recent letter Pütz, Kremer and Grest claim that finite-chain-length effects have significant influence on the single-chain dynamic structure factor S(Q,t)/S(Q) measured by neutron-spin-echo (NSE) technique with regard to results obtained for a polyethylene (PE) melt with a molecular weight of Mw = 36000 g/mol. As a consequence of these finite-length effects, they assert the tube diameter, determined by this NSE measurement in the framework of the reptation model, to be too high by a factor of approx 1.5. We demonstrate that this is by no means the case.

  9. Transmissibility of H-Type Bovine Spongiform Encephalopathy to Hamster PrP Transgenic Mice.

    Directory of Open Access Journals (Sweden)

    Hiroyuki Okada

    Full Text Available Two distinct forms of atypical bovine spongiform encephalopathies (H-BSE and L-BSE can be distinguished from classical (C- BSE found in cattle based on biochemical signatures of disease-associated prion protein (PrPSc. H-BSE is transmissible to wild-type mice-with infected mice showing a long survival period that is close to their normal lifespan-but not to hamsters. Therefore, rodent-adapted H-BSE with a short survival period would be useful for analyzing H-BSE characteristics. In this study, we investigated the transmissibility of H-BSE to hamster prion protein transgenic (TgHaNSE mice with long survival periods. Although none of the TgHaNSE mice manifested the disease during their lifespan, PrPSc accumulation was observed in some areas of the brain after the first passage. With subsequent passages, TgHaNSE mice developed the disease with a mean survival period of 220 days. The molecular characteristics of proteinase K-resistant PrPSc (PrPres in the brain were identical to those observed in first-passage mice. The distribution of immunolabeled PrPSc in the brains of TgHaNSE mice differed between those infected with H-BSE as compared to C-BSE or L-BSE, and the molecular properties of PrPres in TgHaNSE mice infected with H-BSE differed from those of the original isolate. The strain-specific electromobility, glycoform profiles, and proteolytic cleavage sites of H-BSE in TgHaNSE mice were indistinguishable from those of C-BSE, in which the diglycosylated form was predominant. These findings indicate that strain-specific pathogenic characteristics and molecular features of PrPres in the brain are altered during cross-species transmission. Typical H-BSE features were restored after back passage from TgHaNSE to bovinized transgenic mice, indicating that the H-BSE strain was propagated in TgHaNSE mice. This could result from the overexpression of the hamster prion protein.

  10. LEAN dig ikke tilbage

    DEFF Research Database (Denmark)

    Weirsøe, Mathilde

    2011-01-01

    Fit, slankere, hurtigst. Lean-konceptet ruller ind over de offentlige institutioner, hvor det skal trimme og effektivisere arbejdsprocesser. Men hvor er den maksimale grænse for effektivitet? Er der et ’tipping point’, hvor Lean ikke længere motiverer, men snarere udmatter......Fit, slankere, hurtigst. Lean-konceptet ruller ind over de offentlige institutioner, hvor det skal trimme og effektivisere arbejdsprocesser. Men hvor er den maksimale grænse for effektivitet? Er der et ’tipping point’, hvor Lean ikke længere motiverer, men snarere udmatter...

  11. Neutron spin echo studies of the effects of temperature and pressure in a ternary microemulsion

    CERN Document Server

    Kawabata, Y; Seto, H; Takeda, T; Komura, S; Schwahn, D

    2002-01-01

    In order to clarify the self-assembling mechanisms in complex fluids involving amphiphiles, we have investigated dynamic features of amphiphilic membranes and droplets at high temperature and at high pressure in a ternary microemulsion, consisting of AOT, water, and n-decane. A high-pressure cell for neutron spin echo (NSE) experiments has been improved, and the static and dynamic features of droplets are observed in detail by means of small angle neutron scattering and NSE. It is found that the size fluctuation and the diffusion of droplets are enhanced by increasing temperature, while they are suppressed by increasing pressure. (orig.)

  12. Length scale dependence of the dynamic properties of hyaluronic acid solutions in the presence of salt.

    Science.gov (United States)

    Horkay, Ferenc; Falus, Peter; Hecht, Anne-Marie; Geissler, Erik

    2010-12-02

    In solutions of the charged semirigid biopolymer hyaluronic acid in salt-free conditions, the diffusion coefficient D(NSE) measured at high transfer momentum q by neutron spin echo is more than an order of magnitude smaller than that determined by dynamic light scattering, D(DLS). This behavior contrasts with neutral polymer solutions. With increasing salt content, D(DLS) approaches D(NSE), which is independent of ionic strength. Contrary to theoretical expectation, the ion-polymer coupling, which dominates the low q dynamics of polyelectrolyte solutions, already breaks down at distance scales greater than the Debye-Hückel length.

  13. Paulus var kristendommens Lenin

    DEFF Research Database (Denmark)

    Thyssen, Ole

    2017-01-01

    En af Luthers inspirationskilder var Paulus, hvis løsning på lokale problemer fik globale konsekvenser. Han trak en skarp grænse mellem det snavsede kød og den rene sjæl og mellem vantro og tro.......En af Luthers inspirationskilder var Paulus, hvis løsning på lokale problemer fik globale konsekvenser. Han trak en skarp grænse mellem det snavsede kød og den rene sjæl og mellem vantro og tro....

  14. Application of MSS-neutron spin echo spectrometer to pulsed neutron sources

    International Nuclear Information System (INIS)

    Tasaki, S.; Ebisawa, T.; Hino, M.; Kawai, T.

    2001-01-01

    A multilayer spin splitter (MSS) is a neutron device that gives phase difference between field-parallel and -antiparallel spin component of a superposing state. Since the phase difference is equivalent to the Larmor precession angle, MSS enables us to construct a new type of neutron spin echo (NSE) spectrometer. The new NSE spectrometer has its properties that 1. since the phase shift is neutron flight path length, the spectrometer can be drastically small, 2. the neutron spin echo time is proportional to the neutron wavelength. (author)

  15. The diagnostic efficiency of biomarkers in sporadic Creutzfeldt-Jakob disease compared to Alzheimer's disease

    DEFF Research Database (Denmark)

    Bahl, Justyna Maria Czarna; Heegaard, Niels Henrik Helweg; Falkenhorst, Gerhard

    2009-01-01

    ) together with the prion protein gene genotype to discriminate patients with sCJD (n=21) from neurological controls (n=164) and Alzheimer's disease (AD) patients (n=49). Low p-tau/t-tau ratio was the best single marker for sCJD with 90% specificity against neurological controls at 86% sensitivity whilst NSE......Laboratory markers have a prominent place among the diagnostic criteria for sporadic Creutzfeldt-Jakob disease (sCJD). Here we investigate the capability of protein 14-3-3, total-tau (t-tau), threonin-181-phosphorylated tau (p-tau), and neuron-specific enolase (NSE) in cerebrospinal fluid (CSF...

  16. Landmandens verden bliver ustabil

    DEFF Research Database (Denmark)

    Hansen, Henning Otte

    2016-01-01

    Der er udsigt til, at de ustabile priser er kommet for at blive. Fødevarekriser og fjernelse af prisstøtten i EU‘s landbrugspolitik er væsentlige forklaringer. Både landmanden selv, andelsselskaberne og den finansielle sektor kan bidrage til at begrænse ustabiliteten.......Der er udsigt til, at de ustabile priser er kommet for at blive. Fødevarekriser og fjernelse af prisstøtten i EU‘s landbrugspolitik er væsentlige forklaringer. Både landmanden selv, andelsselskaberne og den finansielle sektor kan bidrage til at begrænse ustabiliteten....

  17. Korelasi Peningkatan Kadar Neuron Spesific Enolase dengan Derajat Keparahan dan Luaran Fungsional Pasien Stroke Infark Aterotrombotik Akut

    Directory of Open Access Journals (Sweden)

    Neti Sri Wardiyani

    2010-06-01

    Full Text Available Neuronal damage and decreasing aerobic glicolysis process in ischaemic stroke are caused by lowering level of blood glucose. The amount of neuronal intrasitoplasmic glicolytic enolase enzyme, also known as neuron specific enolase, increases in blood circulation because it is not used anymore in damage neuron. So the mechanism failure in blood-brain barrier, as result of neuronal and cell membrane damage, causes NSE diffusion to extracellular and cerebrospinal fluid, then NSE level increases in blood serum and cerebrospinal fluid in acute cerebral infarction. Elevating NSE level is also connected with infarct volume and the extent of brain damage. The aim of this study was to evaluate connection between upgrading NSE serum level in acute atherothrombotic-stroke infarction patients, level of stroke incompatibility, and functional outcome. The method of study was observational analytic with kohort study. Subjects of study were divided into case group consisted of acute atherothrombotic-stroke infarction patients and control group consisted the healthy person. The data was collected in Hasan Sadikin Hospital between February to August 2008. Evaluating patients was performed to get descriptions on NSE serum level, level stroke incompability measuring by NIHSS scoring at the first time entering the hospital, and Barthel index scoring at seventh day of treatment. This study was analyzed by bivariat analysis using Mann Whitney statistic test and Pearson correlation test. There were 43 patients in each group. There was a significantly difference in NSE serum level on case group (mean was 11.41 [5.07] ng/mL in comparison to those on control group (mean was 8.93 [3.03] ng/mL, p=0.019 . There was a significantly correlation between raising NSE serum level on case group and level of stroke incompatibility measuring by NIHSS scoring and also with functional outcome according to Barthel index scoring. The highest accuration value of NSE serum level was 12 ng

  18. Temperature-induced formation of polymeric nanoparticles: in situ SAXS and QENS experiments

    Czech Academy of Sciences Publication Activity Database

    Sedlák, M.; Falus, P.; Steinhart, Miloš; Gummel, J.; Štěpánek, Petr; Filippov, Sergey K.

    2013-01-01

    Roč. 214, č. 24 (2013), s. 2841-2847 ISSN 1022-1352 R&D Projects: GA ČR GAP108/12/0640 Institutional support: RVO:61389013 Keywords : cryo-transmission electron microscopy (cryo- TEM ) * nanoparticles * neutron-spin-echo (NSE) spectroscopy Subject RIV: CD - Macromolecular Chemistry Impact factor: 2.451, year: 2013

  19. New Species of Orchids from Vietnam

    Directory of Open Access Journals (Sweden)

    Leonid V. Averyanov

    2007-12-01

    Full Text Available Identification of herbarium specimens collected in course of field exploration works in Vietnam during 2005-2007 revealed ten species of orchids new for science. Illustrated descriptions are provided for each discovered species, which are named as Anoectochilus papillosus, Arundina caespitosa, Bulbophyllum paraemarginatum, B. sinhoënse, Cheirostylis foliosa, Goodyera rhombodoides, Liparis rivularis, Oberonia multidentata, O. trichophora and Sunipia nigricans.

  20. [The effect of N-stearoylethanolamine on liver phospholipid composition of rats with insulin resistance caused by alimentary obesity].

    Science.gov (United States)

    Onopchenko, O V; Kosiakova, H V; Horid'ko, T M; Klimashevskyĭ, V M; Hula, N M

    2014-01-01

    We used alimentary obesity-induced insulin resistance (IR) model in rats to investigate the influence of N-stearoylethanolamine on the content of phospholipids and their fatty acid composition. Our results show that prolonged high-fat diet triggers considerable aberrations in the composition of main phospholipids in the liver and can be one of the causes of IR in rats. In particular, the increase of phosphatidylcholine, phosphatidylethanolamine and significant decrease of other phospholipids: lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine and diphosphaglicerol were observed. The levels of monounsaturated (erucic, nervonic, oleic) and polyunsaturated (eicosatrienoic, docosatrienoic, arachidonic) fatty acids were increased; meanwhile the content of diunsaturated acids was decreased. The NSE administration (50 mg/kg of body weight) caused restoration of the phospholipids content in the liver of rats with diet-induced IR that highly correlated with the decrease in plasma insulin level and the improvement of insulin sensitivity. Moreover, the effect of NSE was accompanied by the normalization of fatty acids composition of phospholipids that could be related to modulating influence of NSE on the activity of the main fatty acid desaturases. It is known that the imbalance in phospholipid composition of the rat liver causes substantial metabolic alterations that are associated with the development of IR. Accordingly, the compensations of the imbalance by NSE can help to restore insulin sensitivity, inhibit the development of obesity, IR and type 2 diabetes.

  1. Characterization of trehalose aqueous solutions by neutron spin echo

    Energy Technology Data Exchange (ETDEWEB)

    Branca, C.; Faraone, A.; Magazu' , S.; Maisano, G.; Mangione, A. [Dipartimento di Fisica and INFM, Universita di Messina, PO Box 55, 98166 Messina (Italy); Pappas, C.; Triolo, A. [Hahn-Meitner-Institut, BENSC (NI), Glienicker Strasse, 14109 Berlin (Germany)

    2002-07-01

    The present work reports neutron spin-echo (NSE) results on aqueous mixtures of trehalose, a naturally occurring disaccharide of glucose, which shows an extraordinary bioprotective effectiveness against dehydration and freezing. The aim of the work is to furnish new results on the dynamics of the trehalose/water system on the nano- and picosecond scales. (orig.)

  2. Culture and subculture in transactional letter writing

    DEFF Research Database (Denmark)

    Shaw, Philip; Okamura, Akiko

    2000-01-01

    This study examines the relative contributions of subculture membership and mother-tongue status/target culture membership in writing transactional letters. We examined the letters accompanying articles initially submitted for publication by 26 NSE and 23 NNSE academics, and compared them with ef...

  3. An Intense Nigerian Stock Exchange Market Prediction Using Logistic

    African Journals Online (AJOL)

    analysis derives the stock movement from the stock's own historical value. The historical ... al.,2012), Business and Finance (Pompe et al., 1997, Holmes et al., 1998,. Huang et ... adequacy of ARIMA (1.1.1) model to forecast the NSE index was.

  4. The biomarkers neuron-specific enolase and S100b measured the day following admission for severe accidental hypothermia have high predictive values for poor outcome

    DEFF Research Database (Denmark)

    Wiberg, Sebastian; Kjaergaard, Jesper; Kjærgaard, Benedict

    2017-01-01

    was analyzed for NSE and S100b. Follow-up was conducted after 30days and poor neurologic outcome was defined as a Cerebral Performance Category (CPC) score of 3-5. The predictive value of NSE and S100b was assessed as the area under the receiver-operating characteristics curve (AUC). RESULTS: A total of 34......AIM: The aim of the present study was to assess the ability of the biomarkers neuron-specific enolase (NSE) and S100 calcium-binding protein b (S100b) to predict mortality and poor neurologic outcome after 30days in patients admitted with severe accidental hypothermia. METHODS: Consecutive patients...... in 30 unconscious and/or sedated patients. NSE and S100b achieved AUCs of 0.93 and 0.88, respectively, for prediction of 30day mortality and AUCs of 0.88 and 0.87, respectively, for prediction of poor neurologic outcome. CONCLUSIONS: In patients remaining unconscious the day following admission...

  5. Hydrogen motions and the α-relaxation in glass- forming polymers ...

    Indian Academy of Sciences (India)

    E-mail: wapcolej@sc.ehu.es. Abstract. The combination of molecular dynamics simulations and neutron scattering ... Neutron spin echo (NSE) accesses its Fourier transform Fs(Q, t), and e.g. time of flight or backscattering techniques its .... validating the MD-simulations. In this representation, the suggested dispersion re-.

  6. Against Journal Articles for Measuring Value in University Output

    Science.gov (United States)

    Mbali, C.

    2010-01-01

    The following lines of arguments against the metrics of journal articles is developed: (1) Textual output should no longer be main valued output; (2) Digitalization enables other ways of advancing knowledge; (3) Measures by journal article favours the disciplines of Natural Science and Engineering (NSE) and moulds other disciplines of Social…

  7. System Views on Entrepreneurship – Going in the Right Direction?

    DEFF Research Database (Denmark)

    Christensen, Jesper Lindgaard

    2016-01-01

    The literature on National Systems of Entrepreneurship (NSE) (Acs et al., 2014, 2016) has been claimed to be an important strand of entrepreneurship literature. This paper takes stock of system perspectives on entrepreneurship including recent re-vitalizations of the concept. Based on literature ...

  8. Primary Small Cell Neuroendocrine Carcinoma of Vagina: A Rare Case Report

    Directory of Open Access Journals (Sweden)

    Jignasa N. Bhalodia

    2011-01-01

    Full Text Available Primary small cell neuroendocrine carcinoma of vagina is an extremely rare disease. There have been only 26 previously reported cases in literature. Here, we report a case of primary small cell neuroendocrine carcinoma of vagina. Immunohistochemistry (IHC showed tumor cells positive for synaptophysin, chromogranin, and neuron-specific enolase (NSE.

  9. Modeling sediment concentration of rill flow

    Science.gov (United States)

    Yang, Daming; Gao, Peiling; Zhao, Yadong; Zhang, Yuhang; Liu, Xiaoyuan; Zhang, Qingwen

    2018-06-01

    Accurate estimation of sediment concentration is essential to establish physically-based erosion models. The objectives of this study were to evaluate the effects of flow discharge (Q), slope gradient (S), flow velocity (V), shear stress (τ), stream power (ω) and unit stream power (U) on sediment concentration. Laboratory experiments were conducted using a 10 × 0.1 m rill flume under four flow discharges (2, 4, 8 and 16 L min-1), and five slope gradients (5°, 10°, 15°, 20° and 25°). The results showed that the measured sediment concentration varied from 87.08 to 620.80 kg m-3 with a mean value of 343.13 kg m-3. Sediment concentration increased as a power function with flow discharge and slope gradient, with R2 = 0.975 and NSE = 0.945. The sediment concentration was more sensitive to slope gradient than to flow discharge. The sediment concentration was well predicted by unit stream power (R2 = 0.937, NSE = 0.865), whereas less satisfactorily by flow velocity (R2 = 0.470, NSE = 0.539) and stream power (R2 = 0.773, NSE = 0.732). In addition, using the equations to simulate the measured sediment concentration of other studies, the result further indicated that slope gradient, flow discharge and unit stream power were good predictors of sediment concentration. In general, slope gradient, flow discharge and unit stream power seem to be the preferred predictors for estimating sediment concentration.

  10. Systemically administered AAV9-sTRAIL combats invasive glioblastoma in a patient-derived orthotopic xenograft model

    Directory of Open Access Journals (Sweden)

    Matheus HW Crommentuijn

    2016-01-01

    Full Text Available Adeno-associated virus (AAV vectors expressing tumoricidal genes injected directly into brain tumors have shown some promise, however, invasive tumor cells are relatively unaffected. Systemic injection of AAV9 vectors provides widespread delivery to the brain and potentially the tumor/microenvironment. Here we assessed AAV9 for potential glioblastoma therapy using two different promoters driving the expression of the secreted anti-cancer agent sTRAIL as a transgene model; the ubiquitously active chicken β-actin (CBA promoter and the neuron-specific enolase (NSE promoter to restrict expression in brain. Intravenous injection of AAV9 vectors encoding a bioluminescent reporter showed similar distribution patterns, although the NSE promoter yielded 100-fold lower expression in the abdomen (liver, with the brain-to-liver expression ratio remaining the same. The main cell types targeted by the CBA promoter were astrocytes, neurons and endothelial cells, while expression by NSE promoter mostly occurred in neurons. Intravenous administration of either AAV9-CBA-sTRAIL or AAV9-NSE-sTRAIL vectors to mice bearing intracranial patient-derived glioblastoma xenografts led to a slower tumor growth and significantly increased survival, with the CBA promoter having higher efficacy. To our knowledge, this is the first report showing the potential of systemic injection of AAV9 vector encoding a therapeutic gene for the treatment of brain tumors.

  11. Sex differences in the vaccine-specific and non-targeted effects of vaccines

    DEFF Research Database (Denmark)

    Flanagan, Katie L; Klein, Sabra L; Skakkebaek, Niels E

    2011-01-01

    to eliminate infectious diseases through vaccination programmes, the relative impact of NSE of vaccines on mortality is likely to increase, raising important questions regarding the future of certain vaccine schedules. A diverse group of scientists met in Copenhagen to discuss non-specific and sex...

  12. Ontogeny of neuro-insular complexes and islets innervation in the human pancreas.

    Directory of Open Access Journals (Sweden)

    Alexandra E. Proshchina

    2014-04-01

    Full Text Available The ontogeny of the neuro-insular complexes (NIC and the islets innervation in human pancreas has not been studied in detail. Our aim was to describe the developmental dynamics and distribution of the nervous system structures in the endocrine part of human pancreas. We used doublestaining with antibodies specific to pan-neural markers (neuron-specific enolase (NSE and S100 protein and to hormones of pancreatic endocrine cells. NSE and S100-positive nerves and ganglia were identified in the human fetal pancreas from gestation week (gw 10 onwards. Later the density of S100 and NSE-positive fibers increased. In adults this network was sparse. The islets innervation started to form from gw 14. NSE-containing endocrine cells were identified from gw 12 onwards. Additionally, S100-positive cells were detected both in the periphery and within some of the islets starting at gw 14. The analysis of islets innervation has shown that the fetal pancreas contained neuro-insular complexes and the number of these complexes was reduced in adults. The highest density of neuro-insular complexes is detected during middle and late fetal periods, when the mosaic islets, typical for adults, form. The close integration between the developing pancreatic islets and the nervous system structures may play an important role not only in the hormone secretion, but also in the islets morphogenesis.

  13. Ontogeny of neuro-insular complexes and islets innervation in the human pancreas.

    Science.gov (United States)

    Proshchina, Alexandra E; Krivova, Yulia S; Barabanov, Valeriy M; Saveliev, Sergey V

    2014-01-01

    The ontogeny of the neuro-insular complexes (NIC) and the islets innervation in human pancreas has not been studied in detail. Our aim was to describe the developmental dynamics and distribution of the nervous system structures in the endocrine part of human pancreas. We used double-staining with antibodies specific to pan-neural markers [neuron-specific enolase (NSE) and S100 protein] and to hormones of pancreatic endocrine cells. NSE and S100-positive nerves and ganglia were identified in the human fetal pancreas from gestation week (gw) 10 onward. Later the density of S100 and NSE-positive fibers increased. In adults, this network was sparse. The islets innervation started to form from gw 14. NSE-containing endocrine cells were identified from gw 12 onward. Additionally, S100-positive cells were detected both in the periphery and within some of the islets starting at gw 14. The analysis of islets innervation has shown that the fetal pancreas contained NIC and the number of these complexes was reduced in adults. The highest density of NIC is detected during middle and late fetal periods, when the mosaic islets, typical for adults, form. The close integration between the developing pancreatic islets and the nervous system structures may play an important role not only in the hormone secretion, but also in the islets morphogenesis.

  14. Fraction V of bovine albumin improves the adherence and survival ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-02-04

    Feb 4, 2009 ... 2007; Lopes et al., 2007), neural stem cells and neurons ... In this culture system, stem cells can divide .... disadvantage that albumin can absorb drugs. .... detection of mRNA for NSE in both young (lane 2-6) and adult (lane.

  15. The diagnostic efficiency of biomarkers in sporadic Creutzfeldt-Jakob disease compared to Alzheimer's disease

    DEFF Research Database (Denmark)

    Bahl, J.M.; Heegaard, N.H.; Falkenhorst, G.

    2009-01-01

    ) together with the prion protein gene genotype to discriminate patients with sCJD (n=21) from neurological controls (n=164) and Alzheimer's disease (AD) patients (n=49). Low p-tau/t-tau ratio was the best single marker for sCJD with 90% specificity against neurological controls at 86% sensitivity whilst NSE...

  16. Characterization of trehalose aqueous solutions by neutron spin echo

    CERN Document Server

    Branca, C; Magazù, S; Maisano, G; Mangione, A; Pappas, C; Triolo, A

    2002-01-01

    The present work reports neutron spin-echo (NSE) results on aqueous mixtures of trehalose, a naturally occurring disaccharide of glucose, which shows an extraordinary bioprotective effectiveness against dehydration and freezing. The aim of the work is to furnish new results on the dynamics of the trehalose/water system on the nano- and picosecond scales. (orig.)

  17. Tilsætning af uorganisk og organisk kobber til smågrisefoder

    DEFF Research Database (Denmark)

    Maribo, Hanne; Poulsen, Hanne Damgaard

    2011-01-01

    Et højt indhold af kobber i foderet udgør en risiko for belastning af miljøet, og en begrænsning i udledningen af kobber er derfor ønskelig. Brug af organisk bundet kobber (kobberlysin) som alternativ til uorganisk kobber (kobbersulfat) i foderblandinger kan muligvis begrænse det totale forbrug af...

  18. License - Trypanosomes Database | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us Trypanoso... Attribution-Share Alike 2.1 Japan . If you use data from this database, please be sure attribute this database as follows: Trypanoso...nse Update History of This Database Site Policy | Contact Us License - Trypanosomes Database | LSDB Archive ...

  19. View Presentation

    Indian Academy of Sciences (India)

    Navakant Bhat

    Mere Glucose testing is insufficient for diabetes management ... analytical information.” “A biosensor should be clearly distinguished from a bio-analytical system ... Ref : All about Albumin:Biochemistry,Genetics and Medical Applications by Theodore Peters Jr., ACADEMIC PRESS .... Screen Printer in CeNSE Packaging Lab.

  20. M Moringa a extra eff acts us fects o sed in on etha sugarc anolic ...

    African Journals Online (AJOL)

    sunny

    wine quality a. Juice clarific yces cerevisia. N of the most. United States estimated approximately. 2014). In this . After extrac and insoluble author. E-mail: that this article ..... nol industry, ors during ferm nse-quently e re 1. Interaction mentation speed. Brazil; Season letters compare. , TRS, TPC a e TRS values ant reduction.

  1. TQM, strategic quality management and quality costs

    DEFF Research Database (Denmark)

    Dahlgaard, Jens Jørn

    1995-01-01

    I artiklen kombineres definitionen af kvalitetsomkostninger med definitionen på TQM og strategisk kvalitetsledelse. Der foreslåes en ny metode til at estimere nedre grænse for kvalitetsomkostningerne, og metodens fordele og svagheder diskuteres. Det viser endvidere, hvordan kvalitetsomkostninger ...

  2. The GEWEX LandFlux project: evaluation of model evaporation using tower-based and globally-gridded forcing data

    KAUST Repository

    McCabe, Matthew; Ershadi, Ali; Jimenez, C.; Miralles, Diego G.; Michel, D.; Wood, E. F.

    2015-01-01

    representing the R2, RMSD and Nash-Sutcliffe Efficiency (NSE) and respectively. PM-Mu (0.51; 78 W m−2; 0.45) tended to underestimate fluxes, while SEBS (0.72; 101 W m−2; 0.24) overestimated values relative to observations

  3. Information Technologist (The) - Vol 10, No 1 (2013)

    African Journals Online (AJOL)

    Utilization of electronic books (e-books) by Higher National Diploma (HND) students in Nigerian Library School: The Federal Polytechnic, Nekede experience · EMAIL FULL TEXT EMAIL FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. J Nse, 27-32 ...

  4. Citations to Conference Papers Indicate They Are Declining in Importance across All Discipline Areas. A Review of: Lisée, Cynthia, Vincent Larivière and Eric Archambault. ‚Conference Proceedings as a Source of Scientific Information: A Bibliometric Analysis.‛ Journal of the American Society for Information Science and Technology 59.11 (2008: 1776-84.

    Directory of Open Access Journals (Sweden)

    Gaby Haddow

    2009-06-01

    Full Text Available Objective – To compare the impact and ageing of conference proceedings with that of scientific literature in general, as reflected in citation characteristics.Design – Citation analysis.Setting – Thomson’s Science Citation Index, Social Science Citation Index, and Arts and Humanities Citation Index (CD-ROM version.Subjects – Conference proceedings citations.Methods – The Thomson citation indexes were searched to identify all citations to conference proceedings in natural sciences and engineering (NSE and social sciences and humanities (SSH from 1980 to 2005. Keywords in English, Spanish, Italian and German, truncated terms (such as ‘bienn’, single letters (such as P, and numbers were combined to retrieve all possible citations. Additional filters to exclude citations to publications other than proceedings were applied to the P search results, which had accounted for 75% of the total results. The references remaining in the P search set were validated using Google Scholar and WorldCat. Finally, two random samples of 1,000 references were checked manually to determine the extent of false positives and false negatives in the results.Main Results – The study’s findings are presented for NSE and SSH separately, with 1.7% of NSE citations and 2.5% of SSH citations referring to conference proceedings. The total number of citations to proceedings has increased over the period 1980-2005, however, citations to proceedings in NSE and SSH as a proportion of all citations decreased during this time. A small increase in the average number of proceedings citations per paper was found for NSE and SSH. When this increase is compared to the overall increase in references per paper over this period, the share of proceedings citations per paper has decreased. Of all fields in NSE and SSH, only engineering has increased the proportion of proceedings citations, rising from 7% to 10% in the period studied. In 2005, the share of proceedings

  5. Socialpædagogisk kompetence

    DEFF Research Database (Denmark)

    Wahlgren, Bjarne; Mariager-Anderson, Kristina; Hovmand Sørensen, Sia

    2015-01-01

    Behovet for socialpædagogisk kompetence var overskriften på en artikel i Voksenuddannelses jubilæumsnummer i april 2012. I artiklen blev der stillet spørgsmålet: Kan mere fokus på kursisternes trivsel og udbytte begrænse frafald på VUC? I artiklen blev der omtalt et udviklingsarbejde, der omfatte...

  6. Calibration of a Field-Scale Soil and Water Assessment Tool (SWAT Model with Field Placement of Best Management Practices in Alger Creek, Michigan

    Directory of Open Access Journals (Sweden)

    Katherine R. Merriman

    2018-03-01

    Full Text Available Subwatersheds within the Great Lakes “Priority Watersheds” were targeted by the Great Lakes Restoration Initiative (GLRI to determine the effectiveness of the various best management practices (BMPs from the U.S. Department of Agriculture-Natural Resources Conservation Service National Conservation Planning (NCP Database. A Soil and Water Assessment Tool (SWAT model is created for Alger Creek, a 50 km2 tributary watershed to the Saginaw River in Michigan. Monthly calibration yielded very good Nash–Sutcliffe efficiency (NSE ratings for flow, sediment, total phosphorus (TP, dissolved reactive phosphorus (DRP, and total nitrogen (TN (0.90, 0.79, 0.87, 0.88, and 0.77, respectively, and satisfactory NSE rating for nitrate (0.51. Two-year validation results in at least satisfactory NSE ratings for flow, sediment, TP, DRP, and TN (0.83, 0.54, 0.73, 0.53, and 0.60, respectively, and unsatisfactory NSE rating for nitrate (0.28. The model estimates the effect of BMPs at the field and watershed scales. At the field-scale, the most effective single practice at reducing sediment, TP, and DRP is no-tillage followed by cover crops (CC; CC are the most effective single practice at reducing nitrate. The most effective BMP combinations include filter strips, which can have a sizable effect on reducing sediment and phosphorus loads. At the watershed scale, model results indicate current NCP BMPs result in minimal sediment and nutrient reductions (<10%.

  7. Personer med handicap

    DEFF Research Database (Denmark)

    Larsen, Brian; Jonassen, Anders Bruun; Høgelund, Jan

    Regeringens Arbejdsmarkedskommission kommmer medio 2009 med forslag til, hvordan den samlede arbejdsindsats varigt kan øges gennem reformer på arbejdsmarkedet. En måde det kan ske på, er ved at begrænse antallet af personer, der står uden for arbejdsmarkedet som følge af helbredsproblemer. Rappor...

  8. S-100b and neuron-specific enolase in patients with fulminant hepatic failure

    DEFF Research Database (Denmark)

    Strauss, Gitte Irene; Christiansen, Michael; Møller, Kirsten

    2001-01-01

    , the cerebral flux of S-100b and NSE was measured. We included 35 patients with FHF, 6 patients with acute on chronic liver disease (AOCLD), 13 patients with cirrhosis of the liver without hepatic encephalopathy, and 8 healthy subjects. Blood samples were obtained from catheters placed in the radial artery...

  9. Larmor labeling by time-gradient magnetic fields

    International Nuclear Information System (INIS)

    Ioffe, Alexander; Bodnarchuk, Victor; Bussmann, Klaus; Mueller, Robert

    2007-01-01

    The Larmor labeling of neutrons, due to the Larmor precession of neutron spin in a magnetic field, opens the unique possibility for the development of neutron spin-echo (NSE) based on neutron scattering techniques, featuring an extremely high energy (momentum) resolution. Here, we present the experimental proof of a new method of the Larmor labeling using time-gradient magnetic fields

  10. Assessment of the Environmental Fate of the Herbicides Flufenacet and Metazachlor with the SWAT Model.

    Science.gov (United States)

    Fohrer, Nicola; Dietrich, Antje; Kolychalow, Olga; Ulrich, Uta

    2014-01-01

    This study aims to assess the environmental fate of the commonly used herbicides flufenacet and metazachlor in the Northern German Lowlands with the ecohydrological Soil and Water Assessment Tool (SWAT model) and to test the sensitivity of pesticide-related input parameters on the modeled transport dynamics. The river discharge of the Kielstau watershed was calibrated (Nash-Sutcliffe efficiency [NSE], 0.83; = 0.84) and validated (NSE, 0.76; = 0.77) for a daily time step. The environmental fate of metazachlor (NSE, 0.68; = 0.62) and flufenacet (NSE, 0.13; = 0.51) was simulated adequately. In comparison to metazachlor, the simulated flufenacet concentration and loads show a lower model efficiency due to the weaker simulation of the stream flow. The in-stream herbicide loads were less than 0.01% of the applied amount in the observed time period and thus not in conflict with European Environmental Legislation. The sensitivity analysis showed that, besides the accurate simulation of stream flow, the parameterization of the temporal and spatial distribution of the herbicide application throughout the watershed is the key factor for appropriate modeling results, whereas the physicochemical properties of the pesticides play a minor role in the modeling process. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  11. Aktietilbagekøb og selvfinansierede aktiekøb

    DEFF Research Database (Denmark)

    Werlauff, Erik; Larsen, Luise Guldbæk; Larsen, Dorte Riskjær

    Efter selskabsreformen 2010 kan både aktie- og anpartsselskaber opkøbe egne aktier eller anparter ("kapitalandele") langt ud over den tidligere grænse på 10 pct. af selskabskapitalen (og transaktionen var tidligere helt forbudt for anpartsselskaber). Dette giver en lang række nye muligheder, men ...

  12. NCBI nr-aa BLAST: CBRC-TTRU-01-0739 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0739 ref|YP_506424.1| hypothetical protein NSE_0541 [Neorickettsia sen...netsu str. Miyayama] gb|ABD46153.1| putative membrane protein [Neorickettsia sennetsu str. Miyayama] YP_506424.1 0.086 28% ...

  13. NCBI nr-aa BLAST: CBRC-TTRU-01-0604 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0604 ref|YP_506424.1| hypothetical protein NSE_0541 [Neorickettsia sen...netsu str. Miyayama] gb|ABD46153.1| putative membrane protein [Neorickettsia sennetsu str. Miyayama] YP_506424.1 0.012 26% ...

  14. Unravelling the nature of non-specific effects of vaccines-A challenge for innate immunologists

    DEFF Research Database (Denmark)

    Jensen, Kristoffer Jarlov; Benn, Christine Stabell; van Crevel, Reinout

    2016-01-01

    ) of vaccines, including heterologous T-cell reactivity and innate immune memory or 'trained innate immunity', which involves epigenetic reprogramming of innate immune cells. Here, we review the epidemiological evidence for NSE as well as human, animal and in vitro immunological data that could explain...

  15. Markers of cerebral damage during delirium in elderly patients with hip fracture

    NARCIS (Netherlands)

    van Munster, Barbara C.; Korse, Catharina M.; de Rooij, Sophia E.; Bonfrer, Johannes M.; Zwinderman, Aeilko H.; Korevaar, Johanna C.

    2009-01-01

    ABSTRACT: BACKGROUND: S100B protein and Neuron Specific Enolase (NSE) can increase due to brain cell damage and/or increased permeability of the blood-brain-barrier. Elevation of these proteins has been shown after various neurological diseases with cognitive dysfunction. Delirium is characterized

  16. Early detection of response in small cell bronchogenic carcinoma by changes in serum concentrations of creatine kinase, neuron specific enolase, calcitonin, ACTH, serotonin and gastrin releasing peptide

    DEFF Research Database (Denmark)

    Bork, E; Hansen, M; Urdal, P

    1988-01-01

    Creatine kinase (CK-BB), neuron specific enolase (NSE), ACTH, calcitonin, serotonin and gastrin releasing peptide (GRP) were measured in serum or plasma before and immediately after initiation of treatment in patients with small cell lung cancer (SCC). Pretherapeutic elevated concentrations of CK...

  17. Hård debat gør ikke livet nemt for de østeuropæiske studerende

    DEFF Research Database (Denmark)

    Dahlberg, Mette Ginnerskov

    2017-01-01

    Uddannelsesinternationaliseringen skulle medvirke til at bygge bro mellem forskellige kulturer og nationaliteter. Men den hårde tone i debatten omkring østeuropæiske studerende – og de forskellige livsbetingelser for de studerende på danske universiteter – er med til at hærde den mentale grænse m...

  18. Comparison of net CO2 fluxes measured with open- and closed-path infrared gas analyzers in an urban complex environment

    DEFF Research Database (Denmark)

    Järvi, L.; Mammarella, I.; Eugster, W.

    2009-01-01

    and their suitability to accurately measure CO2 exchange in such non-ideal landscape. In addition, this study examined the effect of open-path sensor heating on measured fluxes in urban terrain, and these results were compared with similar measurements made above a temperate beech forest in Denmark. The correlation...... between the two fluxes was good (R2 = 0.93) at the urban site, but during the measurement period the open-path net surface exchange (NSE) was 17% smaller than the closed-path NSE, indicating apparent additional uptake of CO2 by open-path measurements. At both sites, sensor heating corrections evidently...... improved the performance of the open-path analyzer by reducing discrepancies in NSE at the urban site to 2% and decreasing the difference in NSE from 67% to 7% at the forest site. Overall, the site-specific approach gave the best results at both sites and, if possible, it should be preferred in the sensor...

  19. Pathophysiological consequences of pneumoperitoneum

    NARCIS (Netherlands)

    E.J. Hazebroek (Eric Jasper)

    2002-01-01

    textabstractLaparoscopic surgery has been performed for more than a century, although its nse was mainly restricted to diagnostic purposes 1. 2 Recent developments in instrumental design and methods of visualization have contributed to further implementation of laparoscopic techniques 3 In 1985,

  20. Multi-Composite Bioactive Osteogenic Sponges Featuring Mesenchymal Stem Cells, Platelet-Rich Plasma, Nanoporous Silicon Enclosures, and Peptide Amphiphiles for Rapid Bone Regeneration

    Directory of Open Access Journals (Sweden)

    Dongmei Fan

    2011-06-01

    Full Text Available A novel bioactive sponge was created with a composite of type I collagen sponges or porous poly(e-caprolactone (PCL scaffolds, platelet-rich plasma (PRP, BMP2-loaded nanoporous silicon enclosure (NSE microparticles, mineralizing peptide amphiphiles (PA, and mesenchymal stem cells (MSC. Primary MSC from cortical bone (CB  tissue proved to form more and larger colony units, as well as produce more mineral matrix under osteogenic differentiation, than MSC from bone marrow (BM. Coating pre-treatments were optimized for maximum cell adhesion and mineralization, while a PRP-based gel carrier was created to efficiently deliver and retain MSC and  microparticles within a porous scaffold while simultaneously promoting cell recruitment, proliferation, and angiogenesis. Components and composite sponges were evaluated for osteogenic differentiation in vitro. Osteogenic sponges were loaded with MSC, PRP, PA, and NSE and implanted subcutaneously in rats to evaluate the formation of bone tissue and angiogenesis in vivo. It was found that the combination of a collagen sponge with CB MSC, PRP, PA, and the BMP2-releasing NSE formed the most bone and was most vascularized by four weeks compared to analogous composites featuring BM MSC or PCL or lacking PRP, PA, and NSE. This study indicates that CB MSC should be considered as an alternative to marrow as a source of stem cells, while the PRP-PA cell and microparticle delivery system may be utilized for diverse tissue engineering applications.

  1. Angela Merkel pani saksa turistid rõõmust kiljuma ja peatas rongid / Eve Heinla

    Index Scriptorium Estoniae

    Heinla, Eve, 1966-

    2008-01-01

    Saksamaa liidukantsleri Angela Merkeli visiidist Tallinnasse, kohtumisest peaminister Andrus Ansipi ja president Toomas Hendrik Ilvesega. Vt. samas: Ilves: Euroopa seisab otsustamispiiril. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu; Saksa liidukantsler: NATO kaitseb Eestit; Kas turvamisel kasutati ka mõttetut imevitsa?

  2. Angela Merkel viibis kiirvisiidil Tallinnas

    Index Scriptorium Estoniae

    2008-01-01

    26. augustil 2008 Tallinnas ühepäevasel visiidil viibinud Saksamaa liidukantsler Angela Merkel kohtus president Toomas Hendrik Ilvese ja peaminister Andrus Ansipiga ning pidas loengu Kumu auditooriumis. Nii Eesti president kui Saksamaa liidukantsler hindasid Venemaa tegevust Gruusia vastu kui lubamatut agressiooni suveräänse riigi vastu

  3. The effect of N-stearoylethanolamine on liver phospholipid composition of rats with insulin resistance caused by alimentary obesity

    Directory of Open Access Journals (Sweden)

    O. V. Onopchenko

    2014-02-01

    Full Text Available We used alimentary obesity-induced insulin resistance (IR model in rats to investigate the influence of N-stearoylethanolamine on the content of phospholipids and their fatty acid composition. Our results show that prolonged high-fat diet triggers considerable aberrations in the composition of main phospholipids in the liver and can be one of the causes of IR in rats. In particular, the increase of phosphatidylcholine, phosphatidylethanolamine and significant decrease of other phospholipids: lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, phosphatidylinositol, phosphatidylserine and diphosphaglicerol were observed. The levels of monounsaturated (erucic, nervonic, oleic and polyunsaturated (eicosatrienoic, docosatrienoic, arachidonic fatty acids were increased; meanwhile the content of diunsaturated acids was decreased. The NSE administration (50 mg/kg of body weight caused restoration of the phospholipids content in the liver of rats with diet-induced IR that highly correlated with the decrease in plasma insulin level and the improvement of insulin sensitivity. Moreover, the effect of NSE was accompanied by the normalization of fatty acids composition of phospholipids that could be related to modulating influen­ce of NSE on the activity of the main fatty acid desaturases. It is known that the imbalance in phospholipid composition of the rat liver causes substantial metabolic alterations that are associated with the development of IR. Accordingly, the compensations of the imbalance by NSE can help to restore insulin sensitivity, inhibit the development of obesity, IR and type 2 diabetes.

  4. Calibration of a field-scale Soil and Water Assessment Tool (SWAT) model with field placement of best management practices in Alger Creek, Michigan

    Science.gov (United States)

    Merriman-Hoehne, Katherine R.; Russell, Amy M.; Rachol, Cynthia M.; Daggupati, Prasad; Srinivasan, Raghavan; Hayhurst, Brett A.; Stuntebeck, Todd D.

    2018-01-01

    Subwatersheds within the Great Lakes “Priority Watersheds” were targeted by the Great Lakes Restoration Initiative (GLRI) to determine the effectiveness of the various best management practices (BMPs) from the U.S. Department of Agriculture-Natural Resources Conservation Service National Conservation Planning (NCP) Database. A Soil and Water Assessment Tool (SWAT) model is created for Alger Creek, a 50 km2 tributary watershed to the Saginaw River in Michigan. Monthly calibration yielded very good Nash–Sutcliffe efficiency (NSE) ratings for flow, sediment, total phosphorus (TP), dissolved reactive phosphorus (DRP), and total nitrogen (TN) (0.90, 0.79, 0.87, 0.88, and 0.77, respectively), and satisfactory NSE rating for nitrate (0.51). Two-year validation results in at least satisfactory NSE ratings for flow, sediment, TP, DRP, and TN (0.83, 0.54, 0.73, 0.53, and 0.60, respectively), and unsatisfactory NSE rating for nitrate (0.28). The model estimates the effect of BMPs at the field and watershed scales. At the field-scale, the most effective single practice at reducing sediment, TP, and DRP is no-tillage followed by cover crops (CC); CC are the most effective single practice at reducing nitrate. The most effective BMP combinations include filter strips, which can have a sizable effect on reducing sediment and phosphorus loads. At the watershed scale, model results indicate current NCP BMPs result in minimal sediment and nutrient reductions (<10%).

  5. Goinfo.dk

    DEFF Research Database (Denmark)

    Sørensen, Birgitte Holm

    Rapporten sætter fokus på goinfo.dk, som er en læringsplatform til udvikling af elevers informationskompetence. Platformen indeholder nogle værktøjer og introduktioner, som kan anvendes til at afklare, kategorisere og afgrænse et emne, til at søge information på internettet, til at ordne et...

  6. Increased serum neuron specific enolase concentrations in patients with hyperglycemic cortical ischemic stroke

    NARCIS (Netherlands)

    Elting, JW; De Keyser, J; Sulter, G.

    1998-01-01

    A detrimental effect of hyperglycemia in ischemic brain has been demonstrated in laboratory experiments and it has been found that hyperglycemia in ischemic stroke is a predictor of poor outcome. We determined serum neuron specific enolase (NSE) concentrations in 41 consecutive patients with a

  7. The level of neuron-specific enolase and S-100 protein in the cerebrospinal fluid of patients with acute bacterial meningitis

    Directory of Open Access Journals (Sweden)

    A. V. Sokhan

    2016-08-01

    Full Text Available Aim. To evaluate the diagnostic and prognostic role of neuron-specific enolase (NSE and S-100 protein levels in cerebrospinal fluid (CSF of patients with acute bacterial meningitis in the course of the disease. Materials and Methods. 54 cases of acute bacterial meningitis were analyzed, among them – 26 with pneumococcal and 28 with meningococcal etiology. Patients were divided into groups depending on the severity and etiology of disease. In addition to routine laboratory methods, we analyzed the CSF levels of S-100 protein and NSE at admission and after 10 – 12 days of treatment. 12 patients with acute respiratory infections and meningism were examined as a comparison group. Results. In all patients with acute bacterial meningitis CSF NSE and protein S-100 levels were significantly higher than in the control group (P <0,05. CSF neuro specific proteins level was in direct dependence on severity of the disease, and in patients with severe disease was significantly higher than in patients with moderate severity and in the control group (P <0,01. After 10 – 12 days of treatment, the level of the NSE and S-100 protein decreased, but in severe cases was still higher than in the control group (P <0,05. Conclusions. Increased cerebrospinal fluid NSE and S – 100 protein levels shows the presence and value of neurons and glial cells damage in patients with acute bacterial meningitis. CSF S-100 protein and neuron-specific enolase levels help to determine the severity of neurons destruction and glial cells in patients with acute bacterial meningitis. Level of neurospecific protein is in direct proportion to the severity of the disease and is the highest in patients with severe cases (P<0,05. It confirms the diagnostic and prognostic value of CSF neurospecific protein determination in patients with bacterial meningitis.

  8. Serum Neuron-Specific Enolase, Biogenic Amino-Acids and Neurobehavioral Function in Lead-Exposed Workers from Lead-Acid Battery Manufacturing Process

    Directory of Open Access Journals (Sweden)

    K Ravibabu

    2015-01-01

    Full Text Available Background: The interaction between serum neuron-specific enolase (NSE, biogenic amino-acids and neurobehavioral function with blood lead levels in workers exposed to lead form lead-acid battery manufacturing process was not studied. Objective: To evaluate serum NSE and biogenic amino-acids (dopamine and serotonin levels, and neurobehavioral performance among workers exposed to lead from lead-acid storage battery plant, and its relation with blood lead levels (BLLs. Methods: In a cross-sectional study, we performed biochemical and neurobehavioral function tests on 146 workers exposed to lead from lead-acid battery manufacturing process. BLLs were assessed by an atomic absorption spectrophotometer. Serum NSE, dopamine and serotonin were measured by ELISA. Neurobehavioral functions were assessed by CDC-recommended tests—simple reaction time (SRT, symbol digit substitution test (SDST, and serial digit learning test (SDLT. Results: There was a significant correlation (r 0.199, p<0.05 between SDST and BLL. SDLT and SRT had also a significant positive correlation (r 0.238, p<0.01. NSE had a negative correlation (r –0.194, p<0.05 with serotonin level. Multiple linear regression analysis revealed that both SRT and SDST had positive significant associations with BLL. SRT also had a positive significant association with age. Conclusion: Serum NSE cannot be used as a marker for BLL. The only domain of neurobehavioral function tests that is affected by increased BLL in workers of lead-acid battery manufacturing process is that of the “attention and perception” (SDST.

  9. Habilidades intelectuales de niños indígenas de las etnias Tenek y Nahúa que viven en condiciones de pobreza y desnutrición

    Directory of Open Access Journals (Sweden)

    Raúl Morales Villegas

    2014-11-01

    Full Text Available Las poblaciones indígenas en México son las más vulnerables, debido a que viven en condiciones de pobreza extrema y menor cumplimiento de sus derechos fundamentales. Se ha demostrado que los niños son el grupo que recibe mayor impacto sobre el desarrollo psicológico y físico.El presente trabajo tiene como objetivo describir la relación entre el nivel socioeconómico (NSE, habilidades intelectuales evaluadas con la Batería Woodcock-Muñoz y desnutrición en niños indígenas Tenek y Nahúa que viven en condiciones alta marginación. Los resultados muestran diferencias significativas entre los niños y niñas de nivel NSE bajo, NSE muy bajo y el rendimiento en las pruebas cognitivas que evalúan habilidad intelectual, inteligencia cristalizada e inteligencia visual. Respecto de los indicadores de desnutrición se observó una diferencia estadísticamente significativa para desnutrición aguda entre el NSE bajo y NSE muy bajo. Los efectos del nivel socioeconómico sobre las habilidades intelectuales y el estado nutricional han sido demostrados ampliamente, pero pocos estudios se han realizado con indígenas. Los hallazgos con estas poblaciones son un llamado urgente para las políticas públicas, considerando que el decremento en puntuaciones de habilidades intelectuales, y la desnutrición, en mediano y largo plazo representan graves costos en la calidad de vida, economía y desarrollo humano.

  10. Género y Nivel Socioeconómico de los Niños: Expectativas del Docente en Formación Gender and Socioeconomic Status of Children: Pre-Service Teachers' Expectations

    Directory of Open Access Journals (Sweden)

    M. Francisca del Río

    2010-11-01

    Full Text Available Se puso a prueba empírica el efecto del género y nivel socioeconómico (NSE de los alumnos en las expectativas de los profesores en formación. Participaron 108 estudiantes de pedagogía, los que leyeron una historia acerca de un estudiante hipotético que presentaba problemas conductuales y de rendimiento. Se manipuló el sexo y NSE del estudiante, creando 4 condiciones. Los participantes percibieron que los estudiantes de NSE bajo tenían peores características personales y un futuro académico menos promisorio, no encontrándose diferencias por género. Los participantes de último año de carrera presentaron expectativas más positivas para los niños de NSE bajo que los de primer año. Las expectativas más negativas de los estudiantes de pedagogía respecto de los estudiantes de NSE bajo podrían contribuir a explicar la baja eficacia de los profesores en escuelas vulnerables.The effect of student gender and socioeconomic status (SES on pre-service teachers' expectations of students was examined empirically. Participants were 108 pre-service teachers who read a scenario about a hypothetical student with academic and behavioral challenges. The gender and SES of the student were varied to create 4 conditions. Pre-service teachers perceived that low SES students have less promising futures and more negative personal characteristics, and demonstrated no differences about gender. Senior students had more optimistic expectations for low SES children than freshmen students did. Findings suggest that pre-service teachers are likely to develop negative expectations toward low SES students. These preconceived attitudes may help explain why teacher efficacy tends to be lower in economically disadvantaged schools.

  11. First evidence for glial pathology in late life minor depression:S100B is increased in males with minor depression

    Directory of Open Access Journals (Sweden)

    Maryna ePolyakova

    2015-10-01

    Full Text Available Minor depression is diagnosed when a patient suffers from two to four depressive symptoms for at least two weeks. Though minor depression is a widespread phenomenon, its pathophysiology has hardly been studied. To get a first insight into the pathophysiological mechanisms underlying this disorder we assessed serum levels of biomarkers for plasticity, glial and neuronal function: brain-derived neurotrophic factor (BDNF, S100B and neuron specific enolase (NSE. 27 subjects with minor depressive episode and 82 healthy subjects over 60 years of age were selected from the database of the Leipzig population-based study of civilization diseases (LIFE. Serum levels of BDNF, S100B and NSE were compared between groups, and correlated with age, body-mass index, and degree of white matter hyperintensities (score on Fazekas scale. S100B was significantly increased in males with minor depression in comparison to healthy males, whereas other biomarkers did not differ between groups (p=0.10-0.66. NSE correlated with Fazekas score in patients with minor depression (r=0.436, p=0.048 and in the whole sample (r=0.252, p=0.019. S100B correlated with body mass index (r=0.246, p=0.031 and with age in healthy subjects (r=0.345, p=0.002. Increased S100B in males with minor depression, without alterations in BDNF and NSE, supports the glial hypothesis of depression. Correlation between white matter hyperintensities and NSE underscores the vascular hypothesis of late life depression.

  12. Theory of Quasi-Equilibrium Nucleosynthesis and Applications to Matter Expanding from High Temperature and Density

    Science.gov (United States)

    Meyer, Bradley S.; Krishnan, Tracy D.; Clayton, Donald D.

    1998-05-01

    Our first purpose is construction of a formal theory of quasi-equilibrium. We define quasi-equilibrium, in its simplest form, as statistical equilibrium in the face of an extra constraint on the nuclear populations. We show that the extra constraint introduces a uniform translation of the chemical potentials for the heavy nuclei and derive the abundances in terms of it. We then generalize this theory to accommodate any number of constraints. For nucleosynthesis, the most important constraint occurs when the total number of heavy nuclei Yh within a system of nuclei differs from the number that would exist in nuclear statistical equilibrium (NSE) under the same conditions of density and temperature. Three situations of high relevance are (1) silicon burning, wherein the total number of nuclei exceeds but asymptotically approaches the NSE number; (2) alpha-rich freezeout expansions of high entropy, wherein Yh is less than the NSE number; and (3) expansions from high temperature of low-entropy matter, in which Yh exceeds the NSE number. These are of importance, respectively, within (1) supernova shells, (2) Type II supernova cores modestly outside the mass cut, and (3) Type Ia supernova cores in near-Chandrasekhar-mass events. Our next goal is the detailed analysis of situation (2), the high-entropy alpha-rich neutron-rich freezeout. We employ a nuclear reaction network, which we integrate, to compare the actual abundances with those obtained at the same thermal conditions by the quasi-equilibrium (QSE) theory and by the NSE theory. For this detailed comparison, we choose a high-entropy photon-to-nucleon ratio φ = 6.8, for which we conduct expansions at initial bulk neutron excess η0 = 0.10. We demonstrate that the abundance populations, as they begin expansion and cooling from temperature 10 × 109 K, are characterized by three distinct phases: (1) NSE, (2) QSE having Yh smaller than the NSE value, and (3) final reaction rate-dependent freezeout modifications of the

  13. Comparison of performance of tile drainage routines in SWAT 2009 and 2012 in an extensively tile-drained watershed in the Midwest

    Science.gov (United States)

    Guo, Tian; Gitau, Margaret; Merwade, Venkatesh; Arnold, Jeffrey; Srinivasan, Raghavan; Hirschi, Michael; Engel, Bernard

    2018-01-01

    Subsurface tile drainage systems are widely used in agricultural watersheds in the Midwestern US and enable the Midwest area to become highly productive agricultural lands, but can also create environmental problems, for example nitrate-N contamination associated with drainage waters. The Soil and Water Assessment Tool (SWAT) has been used to model watersheds with tile drainage. SWAT2012 revisions 615 and 645 provide new tile drainage routines. However, few studies have used these revisions to study tile drainage impacts at both field and watershed scales. Moreover, SWAT2012 revision 645 improved the soil moisture based curve number calculation method, which has not been fully tested. This study used long-term (1991-2003) field site and river station data from the Little Vermilion River (LVR) watershed to evaluate performance of tile drainage routines in SWAT2009 revision 528 (the old routine) and SWAT2012 revisions 615 and 645 (the new routine). Both the old and new routines provided reasonable but unsatisfactory (NSE runoff. The calibrated monthly tile flow, surface flow, nitrate-N in tile and surface flow, sediment and annual corn and soybean yield results from SWAT with the old and new tile drainage routines were compared with observed values. Generally, the new routine provided acceptable simulated tile flow (NSE = 0.48-0.65) and nitrate in tile flow (NSE = 0.48-0.68) for field sites with random pattern tile and constant tile spacing, while the old routine simulated tile flow and nitrate in tile flow results for the field site with constant tile spacing were unacceptable (NSE = 0.00-0.32 and -0.29-0.06, respectively). The new modified curve number calculation method in revision 645 (NSE = 0.50-0.81) better simulated surface runoff than revision 615 (NSE = -0.11-0.49). The calibration provided reasonable parameter sets for the old and new routines in the LVR watershed, and the validation results showed that the new routine has the potential to accurately

  14. Water balance estimation in high Alpine terrain by combining distributed modeling and a neural network approach (Berchtesgaden Alps, Germany

    Directory of Open Access Journals (Sweden)

    G. Kraller

    2012-07-01

    process. We tested several ANN setups in different time increments to investigate ANN performance and to examine resulting runoff dynamics of the hydrological model. The ANN with 5-day time increment showed best results in reproducing the observed water storage data (r2 = 0.6. The influx of the 20-day ANN showed best results in the hydrological model correction. The boundary influx in the subbasin improved the hydrological model, as performance increased from NSE = 0.48 to NSE = 0.57 for subbasin Königsseetal, from NSE = 0.22 to NSE = 0.49 for subbasin Berchtesgadener Ache, and from NSE = 0.56 to NSE = 0.66 for the whole catchment within the test period. This combined approach allows distributed quantification of water balance components including subsurface water transfer.

  15. Asociación de hábitos alimentarios y estado nutricional con el nivel socioeconómico en adultos mayores que asisten a un Programa Municipal

    Directory of Open Access Journals (Sweden)

    Fiorella Sánchez-Ruiz

    2014-04-01

    Full Text Available Introducción: La población de adultos mayores se viene incrementando en el país y su problemática es compleja. Es un grupo de riesgo, con problemas de salud y nutrición causados por una serie de factores, entre ellos los socioeconómicos. Objetivos: Determinar la asociación de los hábitos alimentarios y estado nutricional con el nivel socioeconómico (NSE de los adultos mayores que asisten a un Programa Municipal. Diseño: Estudio descriptivo, transversal. Lugar: Programa Municipal del Adulto Mayor en cuatro distritos de Lima Metropolitana. Participantes: Personas mayores de 60 años de ambos sexos. Intervenciones: Se determinó el peso y la talla (por altura de rodilla a 115 personas mayores de 60 años que asistían constantemente al Programa Municipal. Se les aplicó una encuesta sobre hábitos alimentarios y otra para establecer el nivel socioeconómico, ambas previamente validadas. Principales medidas de resultados: Estado nutricional según índice de masa corporal, hábitos alimentarios adecuados según recomendaciones y nivel socioeconómico según categorías de la Escala de Graffar. Resultados: Se encontró una prevalencia de sobrepeso (42,6% y obesidad (19,1%. Más de 60% presentó hábitos alimentarios inadecuados respecto al consumo de carnes (65,2%, lácteos (78,3%, menestras (87,8%, frutas y verduras (64,3%. Los adultos mayores se ubicaron principalmente en el NSE medio bajo (37% y en el NSE medio medio (33%. El estado nutricional no tuvo una asociación estadísticamente significativa con el NSE (p=0,629, mientras que para los hábitos alimentarios, se observó asociación con el NSE respecto al consumo de carnes (p=0,003, cereales y tubérculos (p=0,038 y de frutas y verduras (p=0,001. Conclusiones: En nuestros sujetos de estudio se evidenció asociación entre los hábitos alimentarios con el NSE, pero no así para con el estado nutricional.

  16. 慢性复合应激对食管肿瘤大鼠模型细胞免疫及肿瘤标志物的影响%Effects of Chronic Composite Stress on Cell Immunity and Tumor Marker in Esophageal Neoplasia Model Rats

    Institute of Scientific and Technical Information of China (English)

    王继云; 张俊权; 张建伟; 王建军; 刘本刚; 李万刚

    2012-01-01

    目的 研究慢性复合应激对食管肿瘤大鼠的细胞免疫和肿瘤标志物的影响.方法 选用Wistar 大鼠,制作食管肿瘤大鼠模型,造模成功后,将40只食管肿瘤大鼠随机分为两组,每组20例,一组行4周慢性复合应激,一组正常饲养.观察实验组与对照组应激前后及组间各指标变化,分析慢性复合应激对大鼠细胞免疫功能及肿瘤标志物的影响.结果 40只患食管肿瘤的Wistar大鼠实验前的CD3+、CD4+、CD4 +/CD8+值分别为(65.37±4.51 )μg/L、(30.68±5.40)μg/L、(1.37±0.22)μg/L,实验组应激后值分别为(82.67±5.55)μg/L、(17.81±1.53)μg/L、(0.84±0.12)μg/L,与应激前比较差异有统计学意义(P<0.05).对照组(67.14±1.49) μg/L、(35.70±5.72)μg/L、(1.31±0.15)μg/L,与实验前接近,差异无统计学意义(P>0.05).实验组与对照组比较差异有统计学意义(P<0.01).肿瘤标志物实验前CEA的含量为(4.85±0.93)μg/L,SCC的含量为(1.82±0.61)μg/L,实验组应激后CEA和SCC分别为(6.82±1.06)μg/L和(3.12±1.21 )μg/L,与实验前比较差异有统计学意义(P<0.05),与对照组比较差异亦有统计学意义.其余指标差异均无统计学意义(P>0.05).结论 慢性复合应激导致肿瘤大鼠细胞免疫力下降,对肿瘤进展有一定影响.%Objective To study the effect of multiple stresses on the changes of cellular immunity and tumor marker in oesophageal neoplasia of rats by animal modeling. Methods Wistar rats were chosen for experiment and pathological examination fully confirmed the occurrence of oesophageal cancer in the examined rats. After success of modeling.40 rats with oesophageal neoplasia were randomly divided into two groups,with twenty in each group. One group were treated with chronic composite stress. The other group was used as control,and normally feed. Before the start of experiment,T-lymphocyte subsets,I. E. CD3+ ,CD4 + ,CD4 + /CD8 + and tumor marker,I, e. CEA,CA19-9,CA724,CYFRA21-1 and SCC in

  17. Physical growth and health related physical fitness in adolescents of differents socio economic status

    Directory of Open Access Journals (Sweden)

    Milton Silva

    2006-06-01

    Full Text Available This study had as objective to compare: the physical growth through the body mass (BM, stature and body mass index (BMI between sex and socio-economic status (SES by age; the health-related physical fitness (HRPFbetween gender and SES; the HRPF for sex and SES with the criterion-referenced proposed by AAHPERD (1988. The sample was composed by 191 boys and 212 girls, with ages between 14.49 and 17.50 years, self-evaluated in 4 or 5 TANNER (1962 stages, students from public and private school from Luis Eduardo Magalhães - BA. The SES classification was made by ANEP (1996 questionnaire, adapted to the high, intermediate and low SES. The HRPF components were measured by the respective tests: body fat (sum of the triceps and calf skinfolds; aerobic endurance (run/walk 1600m; muscular strength/endurance (abdominal; upper body muscular strength/endurance (modified pull-up and flexibility (sit and reach. The analysis of the data was made through the descriptive statistics, analysis of variance one-way and the Scheffé’s post-hoc test (p RESUMO Este estudo teve como objetivos comparar: o crescimento físico através da massa corporal (MC, estatura e índice de massa corporal (IMC entre os gêneros e os níveis sócio-econômicos (NSE por idade; a aptidão físicarelacionada à saúde (AFRS entre os gêneros e os NSE; a AFRS por gênero e NSE com os critérios-referenciado propostos pela AAHPERD (1988. A amostra foi composta por 191 rapazes e 212 moças, com idades entre 14,49 e 17,50 anos, auto-avaliada nos estágios 4 ou 5 de TANNER (1962, estudantes das redes pública e particular de Luís Eduardo Magalhães - BA. A classificação sócio-econômica foi feita pelo questionário da ANEP (1996, adaptado para os NSE alto, médio e baixo. Os componentes da AFRS foram mensurados pelos respectivos testes: gordura corporal (somatório da dobras cutâneas tricipital e panturrilha; aptidão cardiorrespiratória (correr/caminhar 1600m; for

  18. Modeling and simulation of ocean wave propagation using lattice Boltzmann method

    Science.gov (United States)

    Nuraiman, Dian

    2017-10-01

    In this paper, we present on modeling and simulation of ocean wave propagation from the deep sea to the shoreline. This requires high computational cost for simulation with large domain. We propose to couple a 1D shallow water equations (SWE) model with a 2D incompressible Navier-Stokes equations (NSE) model in order to reduce the computational cost. The coupled model is solved using the lattice Boltzmann method (LBM) with the lattice Bhatnagar-Gross-Krook (BGK) scheme. Additionally, a special method is implemented to treat the complex behavior of free surface close to the shoreline. The result shows the coupled model can reduce computational cost significantly compared to the full NSE model.

  19. Clinical values of detection of multiple tumor markers in pleural fluid for diagnosis malignancy

    International Nuclear Information System (INIS)

    Xiao Chuangqing; Jiang Li; Zhou Guanghua; He Yunnan

    2005-01-01

    Objective: To improve the diagnostic accuracy for the differentiation of malignant from tuberculous pleural effusion with determination of multiple tumor markers in pleural fluid. Methods: With a multiple tumor markers combined protein chip diagnostic system, contents of twelve common tumor markers were detected in the chest fluid from 60 patients with malignant pleural effusion and 30 patients with tuberculous pleural effusion. Results: For pulmonary carcinoma related pleural effusion, the contents of four common tumor markers (CEA, NSE, SF, CA125) in chest fluid were significantly higher those in tuberculous related chest fluid. The diagnostic positive rate of combined test of these four marker for malignancy could be as high as 96.7%. Conclusion: Combined determination of chest fluid CEA, NSE, SF, CA125 contents was very sensitive and accurate for differentiation of malignant from tuberculous pleural effusion. (authors)

  20. Detection of five tumor markers in lung cancer by trypsin digestion of sputum method

    International Nuclear Information System (INIS)

    Lin Min; Nong Tianlei; Liu Daying

    2011-01-01

    To explore the detection of five tumor markers by trypsin digestion of sputum in the diagnosis of lung cancer, the samples of sputum in patients with lung cancer and benign lung disease were digested by trypsin and used to measure five tumor markers. The results showed that the sputum were well digested by 6% trypsin at pH8 and no affect on the determination of tumor markers. The CEA, CA125, CA153, CA211 and NSE levels in lung cancer group were significantly higher than that of in benign group (P<0.05). The sputum CEA and CA125 levels were significantly higher than that of the serum levels (P<0.05). The detection of sputum CEA, CA125, CA153, CA211 and NSE levels have clinical value in the diagnosis of lung cancer. When combined with other diagnostic methods,it might be helpful for further diagnosis in non confirmed lung cancer patients. (authors)

  1. Precision timing with liquid ionization calorimeters

    International Nuclear Information System (INIS)

    Benary, O.; Cannon, S.; Cleland, W.; Ferguson, I.; Finley, C.; Gordeev, A.; Gordon, H.; Kistenev, E.; Kroon, P.; Letchouk, M.; Lissauer, D.; Ma, H.; Makowiecki, D.; Maslennikov, A.; McCorkle, S.; Onoprienko, D.; Onuchin, A.; Oren, Y.; Panin, V.; Parsons, J.; Rabel, J.; Radeka, V.; Rogers, L.; Rahm, D.; Rescia, S.; Rutherfoord, J.; Seman, M.; Smith, M.; Sondericker, J. III; Steiner, R.; Stephani, D.; Stern, E.; Stumer, I.; Takai, H.; Themann, H.; Tikhonov, Y.

    1993-01-01

    We present timing measurements performed with a liquid krypton electromagnetic accordion calorimeter, measured in an electron beam over an energy range of 5-20 GeV. A novel discriminator with an amplitude-independent timing response was used to extract the inherently accurate timing information from the calorimeter. As expected, the timing resolution σ τ is observed to vary inversely with the signal amplitude, which is proportional to the deposited energy E. We measure a resolution of σ τ =4.15±0.06 GeV ns/E for a sum of 5x5 towers with dimensions 2.7x2.5 cm 2 each. From this we deduce that the timing resolution for an individual tower is approximately 0.8 GeV ns/E. (orig.)

  2. DeltaFosB induces osteosclerosis and decreases adipogenesis by two independent cell-autonomous mechanisms

    DEFF Research Database (Denmark)

    Kveiborg, Marie; Sabatakos, George; Chiusaroli, Riccardo

    2004-01-01

    establishes that the skeletal phenotype is cell autonomous to the osteoblast lineage and independent of adipocyte formation. It also strongly suggests that the decreased fat phenotype of NSE-DeltaFosB mice is independent of the changes in the osteoblast lineage. In vitro, overexpression of Delta......Osteoblasts and adipocytes may develop from common bone marrow mesenchymal precursors. Transgenic mice overexpressing DeltaFosB, an AP-1 transcription factor, under the control of the neuron-specific enolase (NSE) promoter show both markedly increased bone formation and decreased adipogenesis...... of DeltaFosB on adipocyte differentiation appears to occur at early stages of stem cell commitment, affecting C/EBPbeta functions. It is concluded that the changes in osteoblast and adipocyte differentiation in DeltaFosB transgenic mice result from independent cell-autonomous mechanisms....

  3. Comparison of infiltration models in NIT Kurukshetra campus

    Science.gov (United States)

    Singh, Balraj; Sihag, Parveen; Singh, Karan

    2018-05-01

    The aim of the present investigation is to evaluate the performance of infiltration models used to calculate the infiltration rate of the soils. Ten different locations were chosen to measure the infiltration rate in NIT Kurukshetra. The instrument used for the experimentation was double ring infiltrometer. Some of the popular infiltration models like Horton's, Philip's, Modified Philip's and Green-Ampt were fitted with infiltration test data and performance of the models was determined using Nash-Sutcliffe efficiency (NSE), coefficient of correlation (C.C) and Root mean square error (RMSE) criteria. The result suggests that Modified Philip's model is the most accurate model where values of C.C, NSE and RMSE vary from 0.9947-0.9999, 0.9877-0.9998 to 0.1402-0.6913 (mm/h), respectively. Thus, this model can be used to synthetically produce infiltration data in the absence of infiltration data under the same conditions.

  4. Electrochemical Detecting Lung Cancer-Associated Antigen Based on Graphene-Gold Nanocomposite

    Directory of Open Access Journals (Sweden)

    Zheng Wei

    2017-03-01

    Full Text Available Using a Au nanoparticle/reduced graphene oxide composite (AuNP-RGO, a signal-enhanced electrochemical immunosensor without label was created to detect neuron-specific enolase (NSE. Furthermore, an environmentally-friendly method was developed to prepare AuNP-RGO by employing chitosan (CS, which served as reducing and stabilizing agent. We showed that the sensitivity of the immunosensor designed in this report was remarkably enhanced because of the numerous active sites in the sensor provided by the AuNP-RGO nanostructure. For the quantification of NSE, the immunosensor exhibited a positive linear relationship with the concentration in the range of 0.1 to 2000 ng/mL, where the limit of the detection was 0.05 ng/mL.

  5. Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions

    Directory of Open Access Journals (Sweden)

    Gwon-Soo Jung

    2013-05-01

    Full Text Available In this study, we aimed to compare the morphogenetic andneuronal characteristics between monolayer cells andspheroids. For this purpose, we established spheroid formationby growing SH-SY5Y cells on the hydrophobic surfaces ofthermally-collapsed elastin-like polypeptide. After 4 days ofculture, the relative proliferation of the cells within spheroidswas approximately 92% of the values for monolayer cultures.As measured by quantitative assays for mRNA and proteinexpressions, the production of synaptophysin and neuronspecificenolase (NSE as well as the contents of cell adhesionmolecules (CAMs and extracellular matrix (ECM proteins aremuch higher in spheroids than in monolayer cells. Under theall-trans-retinoic acid (RA-induced differentiation condition,spheroids extended neurites and further up-regulated theexpression of synaptophysin, NSE, CAMs, and ECM proteins.Our data indicate that RA-differentiated SH-SY5Y neurospheroidsare functionally matured neuronal architectures. [BMBReports 2013; 46(5: 276-281

  6. Non-invasive biological dosimetry of the skin

    International Nuclear Information System (INIS)

    Barton, S.; Marks, R.; Charles, M.W.; Wells, J.

    1986-01-01

    Investigations designed to identify a potential biological dosimetry technique to examine the effects of X-ray doses down to 0.1 Gy on human skin, are described. In a variety of parameters assessed, the most important changes observed were a significant depression in epidermal cell production in the basal layer after X-ray doses between 0.5 Gy and 1 Gy and a concomitant reduction in the desquamation rate of corneocytes after doses above 1 Gy. Changes in non-specific esterase (NSE) activity were also observed. Further work is described which applies these results to several non-invasive techniques which may have potential for routine application. Preliminary data from irradiated human skin are presented on the measurement of forced desquamation, the evaluation of NSE activity from hair samples and the evaluation of stratum corneum turnover time using the fluorescent dye, dansyl chloride. (author)

  7. Kan man ventilere sig ud af problemer med forhøjet indhold af PCB i indeluften?

    DEFF Research Database (Denmark)

    Lyng, Nadja

    2016-01-01

    Vi ser stadig, at byggematerialer fra perioden i nogle tilfælde er årsag til forhøjede koncentrationer af PCB i indeklimaet. Sundhedsstyrelsen har derfor fastsat en vejledende aktionsgrænse på 300 ng/ m ³. Ved koncentrationer over denne grænse anbefales midlertidig afhjælpning som øget rengøring og...... ventilation eller udluftning implementeret straks. Er disse tiltag ikke tilstrækkelige til at nedbringe koncentrationen, anbefales det uden unødig forsinkelse at nedbringe koncentrationen ved kildefjernelse eller indkapsling. Ventilation har betydning for PCB-koncentrationen i indeluften, men kan ventilation...... forurenede lokaler. Da PCB-koncentrationen afhænger af temperaturen, er alle koncentrationer korrigeret for temperatur for at kunne vurdere effekten af ventilation alene....

  8. Precursor of transition to turbulence: spatiotemporal wave front.

    Science.gov (United States)

    Bhaumik, S; Sengupta, T K

    2014-04-01

    To understand transition to turbulence via 3D disturbance growth, we report here results obtained from the solution of Navier-Stokes equation (NSE) to reproduce experimental results obtained by minimizing background disturbances and imposing deterministic excitation inside the shear layer. A similar approach was adopted in Sengupta and Bhaumik [Phys. Rev. Lett. 107, 154501 (2011)], where a route of transition from receptivity to fully developed turbulent stage was explained for 2D flow in terms of the spatio-temporal wave-front (STWF). The STWF was identified as the unit process of 2D turbulence creation for low amplitude wall excitation. Theoretical prediction of STWF for boundary layer was established earlier in Sengupta, Rao, and Venkatasubbaiah [Phys. Rev. Lett. 96, 224504 (2006)] from the Orr-Sommerfeld equation as due to spatiotemporal instability. Here, the same unit process of the STWF during transition is shown to be present for 3D disturbance field from the solution of governing NSE.

  9. Estimate of radiation detriment long period after exposure to low doses of ionizing radiation: Chromosomal aberrations in liquidators 6-10 years after the Chernobyl accident

    International Nuclear Information System (INIS)

    Nikiforov, A.M.; Slozina, N.M.; Neronova, E.G.; Kharchenko, T.V.; Drygina, L.B.; Strukov, E.L.

    1997-01-01

    The group of 297 liquidators was cytogenetically investigated 6 - 10 years after the Chernobyl accident. The significantly increased level of chromosomal and chromatid types exchange aberrations was shown. For all subjects questionnaires that provide consideration of known and suspected confounding variables were filled in. The participation in recovery works at the Chernobyl nuclear power station was the only reason for dicentrics and rings rise in liquidators. An investigation of the tumor-specific markers (CEA, AFP, CA19-9, PSA, NSE) was carried out in 56 liquidators simultaneously with chromosomal analysis. The increased level of NSE was found in liquidators bearing the chromosomal aberrations of exchange type. The results of this work let us to consider the liquidators who underwent to low doses of ionizing radiation 6-10 years ago as a detrimental group that needs special scientific and medical attention. (author)

  10. High Dynamic Performance Nonlinear Source Emulator

    DEFF Research Database (Denmark)

    Nguyen-Duy, Khiem; Knott, Arnold; Andersen, Michael A. E.

    2016-01-01

    As research and development of renewable and clean energy based systems is advancing rapidly, the nonlinear source emulator (NSE) is becoming very essential for testing of maximum power point trackers or downstream converters. Renewable and clean energy sources play important roles in both...... terrestrial and nonterrestrial applications. However, most existing NSEs have only been concerned with simulating energy sources in terrestrial applications, which may not be fast enough for testing of nonterrestrial applications. In this paper, a high-bandwidth NSE is developed that is able to simulate...... change in the input source but also to a load step between nominal and open circuit. Moreover, all of these operation modes have a very fast settling time of only 10 μs, which is hundreds of times faster than that of existing works. This attribute allows for higher speed and a more efficient maximum...

  11. Stress-induced rise in serum anti-brain autoantibody levels in the rat.

    Science.gov (United States)

    Andrejević, S; Bukilica, M; Dimitrijević, M; Laban, O; Radulovic, J; Kovacevic-Jovanovic, V; Stanojevic, S; Vasiljevic, T; Marković, B M

    1997-02-01

    Sera from Wistar rats subjected to different stress procedures were tested by ELISA for the presence of autoantibodies with specificity for neuron-specific enolase (NSE) and S100 protein that are preferentially localized in neurons and glia, respectively. Autoantibodies were present in sera of animals before exposure to stress, and raised with age. Anti-NSE and anti-S100 autoantibody levels were increased one day after termination of restraint (2 hours daily, 10 days) and electric tail shock (80 shocks daily, 19 days), and in fifth and tenth week of overcrowding stress. Differences between stressed and control animals were not present one month following restraint and electric tail shock and in twentieth week of overcrowding.

  12. Influencia del nivel socioeconómico sobre el dimorfismo sexual de escolares patagónicos

    Directory of Open Access Journals (Sweden)

    Botterón, Tania Vanesa

    2003-01-01

    Full Text Available La expresión del dimorfismo sexual (DS puede estar condicionada por factores ambientales. El DS en poblaciones humanas se expresa por diferencias somatométricas entre varones y mujeres, que disminuyen o se inhiben bajo condiciones nutricionales deficitarias. El objetivo fue evaluar el DS en el crecimiento corporal en escolares de 6 a 14 años de dos contextos socioeconómicos (NSE1 y (NSE2. Se realizó un estudio tranverso de una muestra de 849 mujeres y 913 varones de la ciudad de Puerto Madryn, Chubut. Se relevaron: peso, estatura total, estatura sentada, perímetro braquial y cefálico, ancho humeral, pliegues subcutáneos tricipital y subescapular y se calculó el IMC. El análisis estadístico consistió en pruebas de ANOVA, p= 0.05. Los resultados fueron dispares de acuerdo a la variable analizada. El peso fue dimórfico a edades tempranas, en tanto que la talla lo fue a partir de los 13 años. La estatura sentada presentó dimorfismo sexual en la mayoría de las categorías etáreas con predominio dispar de un sexo sobre el otro. El perímetro cefálico y el ancho humeral fueron dimórficos, presentando valores mayores en el NSE1. Los pliegues mostraron valores superiores en el NSE2 y para el pliegue tricipital las mujeres tuvieron mayor acumulación de tejido graso. La investigación confirma la influencia que las desigualdades sociales tienen en el crecimiento y salud de la población infantil estudiada, reflejada en las diferencias morfológicas.

  13. N-stearoylethanolamine restores pancreas lipid composition in obesity-induced insulin resistant rats.

    Science.gov (United States)

    Onopchenko, Oleksandra V; Kosiakova, Galina V; Oz, Murat; Klimashevsky, Vitaliy M; Gula, Nadiya M

    2015-01-01

    This study investigates the protective effect of N-stearoylethanolamine (NSE), a bioactive N-acylethanolamine , on the lipid profile distribution in the pancreas of obesity-induced insulin resistant (IR) rats fed with prolonged high fat diet (58% of fat for 6 months). The phospholipid composition was determined using 2D thin-layer chromatography. The level of individual phospholipids was estimated by measuring inorganic phosphorus content. The fatty acid (FA) composition and cholesterol level were investigated by gas-liquid chromatography. Compared to controls, plasma levels of triglycerides and insulin were significantly increased in IR rats. The pancreas lipid composition indicated a significant reduction of the free cholesterol level and some phospholipids such as phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn), phosphatidylinositol (PtdIns), phosphatidylserine (PtdSer) compared to controls. Moreover, the FA composition of pancreas showed a significant redistribution of the main FA (18:1n-9, 18:2n-6, 18:3n-6 and 20:4n-6) levels between phospholipid, free FA, triglyceride fractions under IR conditions that was accompanied by a change in the estimated activities of Δ9-, Δ6-, Δ5-desaturase. Administration of N-stearoylethanolamine (NSE, 50 mg/kg daily per os for 2 weeks) IR rats triggered an increase in the content of free cholesterol, PtdCho and normalization of PtdEtn, PtdSer level. Furthermore, the NSE modulated the activity of desaturases, thus influenced FA composition and restored the FA ratios in the lipid fractions. These NSE-induced changes were associated with a normalization of plasma triglyceride content, considerable decrease of insulin and index HOMA-IR level in rats under IR conditions.

  14. Endometrial Polyps and Benign Endometrial Hyperplasia Present Increased Prevalence of DNA Fragmentation Factors 40 and 45 (DFF40 and DFF45) Together With the Antiapoptotic B-Cell Lymphoma (Bcl-2) Protein Compared With Normal Human Endometria.

    Science.gov (United States)

    Banas, Tomasz; Pitynski, Kazimierz; Mikos, Marcin; Cielecka-Kuszyk, Joanna

    2017-09-13

    DNA fragmentation factor 40 (DFF40) is a key executor of apoptosis. It localizes to the nucleus together with DNA fragmentation factor 45 (DFF45), which acts as a DFF40 inhibitor and chaperone. B-cell lymphoma (Bcl-2) protein is a proven antiapoptotic factor present in the cytoplasm. In this study, we aimed to investigate DFF40, DFF45, and Bcl-2 immunoexpression in endometrial polyps (EPs) and benign endometrial hyperplasia (BEH) tissue compared with that in normal proliferative endometrium (NPE) and normal secretory endometrium (NSE) as well as normal post menopausal endometrium (NAE). This study used archived samples from 65 and 62 cases of EPs and BEH, respectively. The control group consisted of 52 NPE, 54 NSE, and 54 NAE specimens. Immunohistochemistry was used to detect DFF40, DFF45, and Bcl-2. DFF40, DFF45, and Bcl-2 were more highly expressed in the glandular layer of EPs and BEH compared with the stroma, and this was not influenced by menopausal status. Both glandular and stromal expression of DFF40, DFF45, and Bcl-2 were significantly higher in EPs compared with NPE, NSE, and NAE. Glandular BEH tissue showed significantly higher DFF40, DFF45, and Bcl-2 expression than in NPE, NSE, and NAE. No differences in the glandular expression of DFF40, DFF45, and Bcl-2 were observed between EP and BEH tissues, while Bcl-2 stromal expression in BEH was significantly lower than in EPs. Glandular, menopause-independent DFF40, DFF45, and Bcl-2 overexpression may play an important role in the pathogenesis of EPs and BEH.

  15. On-Chip Spyhole Nanoelectrospray Ionization Mass Spectrometry for Sensitive Biomarker Detection in Small Volumes

    Science.gov (United States)

    Zhong, Xiaoqin; Qiao, Liang; Stauffer, Géraldine; Liu, Baohong; Girault, Hubert H.

    2018-03-01

    A polyimide microfluidic chip with a microhole emitter (Ø 10-12 μm) created on top of a microchannel by scanning laser ablation has been designed for nanoelectrospray ionization (spyhole-nanoESI) to couple microfluidics with mass spectrometry. The spyhole-nanoESI showed higher sensitivity compared to standard ESI and microESI from the end of the microchannel. The limits of detection (LOD) for peptide with the spyhole-nanoESI MS reached 50 pM, which was 600 times lower than that with standard ESI. The present microchip emitter allows the analysis of small volumes of samples. As an example, a small cell lung cancer biomarker, neuron-specific enolase (NSE), was detected by monitoring the transition of its unique peptide with the spyhole-nanoESI MS/MS. NSE at 0.2 nM could be well identified with a signal to noise ratio (S/N) of 50, and thereby its LOD was estimated to be 12 pM. The potential application of the spyhole-nanoESI MS/MS in cancer diagnosis was further demonstrated with the successful detection of 2 nM NSE from 1 μL of human serum. Before the detection, the serum sample spiked with NSE was first depleted with immune spin column, then desalted by centrifugal filter device, and finally digested by trypsin, without any other complicated preparation steps. The concentration matched the real condition of clinical samples. In addition, the microchips can be disposable to avoid any cross contamination. The present technique provides a highly efficient way to couple microfluidics with MS, which brings additional values to various microfluidics and MS-based analysis.

  16. Main - RPSD | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ... as rice. Data file File name: rpsd_main_sjis.zip File URL: ftp://ftp.biosciencedbc.jp/archive/rpsd/LATEST/r.../ftp.biosciencedbc.jp/archive/rpsd/LATEST/rpsd_main_utf8.zip File size: 120 KB Simple search URL http://togo...nse Update History of This Database Site Policy | Contact Us Main - RPSD | LSDB Archive ...

  17. Central American Flying Weather

    Science.gov (United States)

    1985-12-01

    CEILING; VISIBILITY; WIND, PRECIPITATIDNc’--." HAZE, SMOKE, TEMPORALE ; MOUNTAIN WAVE; MILITARY METEOROLOGY. 4k- / ’A. bstract; Asummary of~ing weather...1 The " Temporale " ....................................1 Mountain Waves ......................I...............1 Severe Thunderstorms...charts. The for any part of Central America lies in having: Tactical Pilota.e Chart series , produced by the Df -.nse Mapping Agency, is * A good, basic

  18. The Random-Walk Hypothesis on the Indian Stock Market

    OpenAIRE

    Ankita Mishra; Vinod Mishra; Russell Smyth

    2014-01-01

    This study tests the random walk hypothesis for the Indian stock market. Using 19 years of monthly data on six indices from the National Stock Exchange (NSE) and the Bombay Stock Exchange (BSE), this study applies three different unit root tests with two structural breaks to analyse the random walk hypothesis. We find that unit root tests that allow for two structural breaks alone are not able to reject the unit root null; however, a recently developed unit root test that simultaneously accou...

  19. Ab to wat belmos ter stre schus e ess in escule the so entus ( oil L.)

    African Journals Online (AJOL)

    SAM

    diet. Its mine ts are signi ncy of Interna pular in the U market is ab main exporter. 600 tons duri uthor. E-mail: n hat this article r nse. 7 August, 2014. 3. 056 ht of this article ... ater content stage of pla il water def ants at vege psules of var ed at floweri ery profitab pecially for w n rural and u turnover of t mated at 276 medicinal.

  20. Control of Shareholders’ Wealth Maximization in Nigeria

    OpenAIRE

    A. O. Oladipupo; C. O. Okafor

    2014-01-01

    This research focuses on who controls shareholder’s wealth maximization and how does this affect firm’s performance in publicly quoted non-financial companies in Nigeria. The shareholder fund was the dependent while explanatory variables were firm size (proxied by log of turnover), retained earning (representing management control) and dividend payment (representing measure of shareholders control). The data used for this study were obtained from the Nigerian Stock Exchange [NSE] fact book an...

  1. Cécil von Renthe-Fink og grænsespørgsmålet

    DEFF Research Database (Denmark)

    Nissen, Mogens Rostgaard

    2011-01-01

    I slutningen af 1936 kom Cécil von Renthe-Fink til København som ny tysk gesandt i Danmark. Det fik betydning for det dansk-tyske forhold generelt og det fik en særlig betydning for det tyske mindretal i Sønderjylland og dets ønske om en grænseændring. Artiklen forholder sig til Cécil von Renthe-Finks...

  2. Assessment of two loss methods for estimation of surface runoff in Zaafrania urban catchment, North-East of Algeria

    Directory of Open Access Journals (Sweden)

    Dahdouh Yacina

    2018-03-01

    Full Text Available Surface runoff is a major problem in urban catchments; its generation is always related to the amount of effective rainfall dropped over the surface, however in urban catchments the process is considerably altered by the emergence of impervious areas. In this study the Soil Consevation Service – curve number (SCS-CN and the Green–Ampt loss methods were used in rainfall-runoff modelling in the Zaafrania urban catchment which is located in Annaba city in the north east of Algeria. The two loss methods were carried out within Hydrologic Engineering Center – Hydrologic Modelling System (HEC-HMS, the choice of the appropriate method for simulating runoff hydrographs in the study area was made by comparing the simulated hydrographs versus observed data using visual inspection and statistical analysis. The results indicate that SCS-CN loss method fit better in the case of 100 years return period NSE (0.462 than in 10 years NSE (0.346 and the results of calibration of Green–Ampt loss method for the 100 years return period NSE (0.417 provide best fit than the case of 10 years NSE (0.381. Furthermore, the results of both return periods (10 and 100 years of SCS-CN loss method provide best fit than the results of return periods (10 and 100 years of Green–Ampt loss method. It could be concluded that SCS-CN method is preferred to the Green–Ampt method for event based rainfall-runoff modelling.

  3. Den amoralske liberalisme

    DEFF Research Database (Denmark)

    Skov, Christian Houlberg

    2011-01-01

    Fra både højre og venstre skydes der i disse år med skarpt mod liberalismen; i denne artikel anmelder Christian H. Skov Against Liberalism af John Kekes, der søger at påvise liberalismens mangler fra et logisk-filosofisk udgangspunkt, med den grundlæggende påstand, at liberalismens to mål, at fre......, at fremelske autonomi og begrænse ondskab, er uforenelige....

  4. Air Force Personalized Medicine Program Panel: Representative Research at the 59th Medical Wing San Antonio Military Medical Center

    Science.gov (United States)

    2016-05-18

    DNA used to predict individual’s re~nse to cardiac therapeutics &Provide universa l standard sample and ampl icon preparat ion protocol; point of care...coordination of patient evacuation Adverse Childhood Experience Serotonin Transporters and Telomeres: A Gene Environmental Interaction Study of the...Risk of PTSD in Soldiers Biomarkers for Mental Illness and Recovery using Sleep as a Mediator: A Randomized Controlled Trial Characterization of the

  5. Grænsestrategier og transnationale relationer

    DEFF Research Database (Denmark)

    Eilenberg, Michael

    2006-01-01

    I denne artikel ønsker jeg at undersøge betydningen af transnationale etniske relationer for den indonesiske ibanske befolkning, der er bosat i grænselandet, mellem den malaysiske delstat, Sarawak og den indonesiske provins, Vest Kalimantan. Jeg vil diskutere hvordan transnationale etniske...... disse processer, som de kommer til udtryk i form af bl.a. arbejdsmigration på tværs af den internationale grænse til Sarawak, Malaysia. Udgivelsesdato: Juli...

  6. Nitrat i drikkevandet og vores sundhed

    DEFF Research Database (Denmark)

    Hansen, Birgitte; Schullehner, Jörg; Sigsgaard, Torben

    2014-01-01

    Nitrat i drikkevandet er uønsket, da det kan påvirke vores sundhed negativt. Den øvre grænse for hvor meget nitrat der tillades i drikkevandet er fastsat i forhold til risikoen for akut forgiftning med nitrit og blå børn-syndromet. Men nitrat i drikkevandet mistænkes også for at være medvirkende...

  7. Effect of total flavonoid in rabdosia rubescens on tolerant mice models under cerebral anoxia

    Directory of Open Access Journals (Sweden)

    Le Kang

    2017-12-01

    Conclusion: The total flavonoid in rabdosia rubescens can stimulate an endogenous protective mechanism by inducing the release of low levels of cytokines NO and NOS, which reduces the release of serum NSE, relieves the brain tissue ischemia-reperfusion injury, and further improves the protection effect of ischemic preconditioning on brain injury. The damage of brain tissue ischemia and reperfusion, and further improve the ischemia Protective effect of preconditioning on brain injury.

  8. Hvordan håndterer Danmark risikoen for civile tab i sine bombeangreb med F16?

    DEFF Research Database (Denmark)

    Kjærgaard, Steen; Marrup, Karsten

    Danske kampfl y har i de seneste fem år med jævne mellemrum deltaget i angrebsmissioner i Afghanistan, Libyen eller som nu i Irak og Syrien. Der har løbende været fremført kritik fra bl.a. Amnesty International af, om der gøres nok for at begrænse utilsigtede følgeskader, herunder civile tab, i...

  9. Hippocampal kindling alters the concentration of glial fibrillary acidic protein and other marker proteins in rat brain

    DEFF Research Database (Denmark)

    Hansen, A; Jørgensen, Ole Steen; Bolwig, T G

    1990-01-01

    The effect of hippocampal kindling on neuronal and glial marker proteins was studied in the rat by immunochemical methods. In hippocampus, pyriform cortex and amygdala there was an increase in glial fibrillary acidic protein (GFAP), indicating reactive gliosis, and an increase in the glycolytic...... enzyme NSE, suggesting increased anaerobic metabolism. Neuronal cell adhesion molecule (NCAM) decreased in pyriform cortex and amygdala of kindled rats, indicating neuronal degeneration....

  10. Neutron scattering. Lectures of the JCNS laboratory course held at Forschungszentrum Juelich and the research reactor FRM II of TU Munich

    International Nuclear Information System (INIS)

    Brueckel, T.; Heger, G.; Richter, D.; Zorn, R.

    2007-01-01

    The following topics are dealt with: Fourier transform, basic assumptions of quantum mechanics and the Born approximation, symmtery in crystals, neutron sources, neutron elastic scattering and properties, polarized neutron scattering, correlation functions measured by scattering experiments, grazing incidence neutron scattering, neutron diffractometers, small-angle scattewring inelastic crystal spectrometers, time-of-flight spectrometers using NSE, structure determination, inelastic neutron scattering with phonon and magnon excitations, structure of complex fluids and macromolecules, polymer dynamics, magnetism. (HSI)

  11. Biomarkers of brain injury in neonatal encephalopathy treated with hypothermia.

    Science.gov (United States)

    Massaro, An N; Chang, Taeun; Kadom, Nadja; Tsuchida, Tammy; Scafidi, Joseph; Glass, Penny; McCarter, Robert; Baumgart, Stephen; Vezina, Gilbert; Nelson, Karin B

    2012-09-01

    To determine if early serum S100B and neuron-specific enolase (NSE) levels are associated with neuroradiographic and clinical evidence of brain injury in newborns with encephalopathy. Patients who received therapeutic whole-body hypothermia were prospectively enrolled in this observational study. Serum specimens were collected at 0, 12, 24, and 72 hours of cooling. S100B and NSE levels were measured by enzyme linked immunosorbent assay. Magnetic resonance imaging was performed in surviving infants at 7-10 days of life. Standardized neurologic examination was performed by a child neurologist at 14 days of life. Multiple linear regression analyses were performed to evaluate the association between S100B and NSE levels and unfavorable outcome (death or severe magnetic resonance imaging injury/significant neurologic deficit). Cutoff values were determined by receiver operating curve analysis. Newborns with moderate to severe encephalopathy were enrolled (n = 75). Median pH at presentation was 6.9 (range, 6.5-7.35), and median Apgar scores of 1 at 1 minute, 3 at 5 minutes, and 5 at 10 minutes. NSE and S100B levels were higher in patients with unfavorable outcomes across all time points. These results remained statistically significant after controlling for covariables, including encephalopathy grade at presentation, Apgar score at 5 minutes of life, initial pH, and clinical seizures. Elevated serum S100B and NSE levels measured during hypothermia were associated with neuroradiographic and clinical evidence of brain injury in encephalopathic newborns. These brain-specific proteins may be useful immediate biomarkers of cerebral injury severity. Copyright © 2012 Mosby, Inc. All rights reserved.

  12. Clinical value of detection on ser um monocyte chemotactant protein-1 and vascular endothelial cadher in levels in patients with acute cerebral infarction

    Directory of Open Access Journals (Sweden)

    Xia Zhou

    2016-11-01

    Full Text Available Objective: To study the correlation of serum monocyte chemotactant protein-1 (MCP-1 and vascular endothelia cadherin (VE-cadherin levels in patients with acute cerebral infarction, and nerve injury molecules, interleukins and matrix metalloproteinases. Methods: A total of 86 patients with acute cerebral infarction treated in our hospital from April 2012 to October 2015 were selected as the observation group and 50 healthy subjects in the same period treated in our hospital were selected as the control group. The serums were collected and the contents of MCP-1, VE-cadherin, heart-type fatty acid binding protein (H-FABP, S100 calcium binding protein B (S100B, neuron-specific enolase (NSE, interleukin-lb (IL-1b, IL-6, IL-17, IL-18, matrix metalloproteinase-2 (MMP2, MMP3 and MMP9 were measured. Results: The serum contents of MCP-1, VE-cadherin, H-FABP, S100B, NSE, IL-1b, IL- 6, IL-17, IL-18, MMP2, MMP3 and MMP9 in observation group were significantly higher than those of control group. Carotid artery plaque formation and unstable plaque properties will increase the serum contents of MCP-1, VE-cadherin, H-FABP, S100B, NSE, IL-1b, IL-6, IL-17, IL-18, MMP2, MMP3 and MMP9 in patients with cerebral infarction. The serum levels of MCP-1, VE-cadherin and the contents of H-FABP, S100B, NSE, IL-1b, IL-6, IL-17, IL-18, MMP2, MMP3 and MMP9 were positively correlated. Conclusions: The serum levels of VE-cadherin and MCP-1 were significantly increased in patients with acute cerebral infarction. MCP-1 and VE-cadherin can increase the secretion of interleukins and matrix metalloproteinases, which can result in the carotid artery plaque formation, unstable plaque properties and the injury of nerve function.

  13. Taste bud cells and nerves

    OpenAIRE

    武田,正子/内田,暢彦/鈴木,裕子; タケダ,マサコ/ウチダ,ノブヒコ/スズキ,ユウコ; TAKEDA,Masako/UCHIDA,Nobuhiko/SUZUKI,Yuko

    2002-01-01

    Sectioning of glossopharyngeal nerves which innervate the taste buds in the circumvallate papillae caused apoptosis of taste buds, the numbers decreasing and the taste buds disappearing after 11 days. This indicates that gustatory nerves may release a trophic substance that induces and maintains taste buds. Taste bud cells contain neurotrophins, NCAM, NSE, PGP9.5, and NeuroD which are specific markers of neurons. The BDNF and GDNF of neurotrophins, and Trk B and GFRαl of their receptors were ...

  14. Scho d chara ots of ott) infe acteriz cocoy ected w zation yam

    African Journals Online (AJOL)

    SAM

    defen eins extracted fr. 1054 (Wi) and blotted on PVD specific for PR-3 extracted from th ose of their res s following SDS ased resistan. ,3-glucanase activity was ction. Я-1,3- essed in RO1 w accumulati. The low leve e tolerant c me may not h defense agai itinase in the yam at 8 dp nse-related in. Nyoche rom the roots of.

  15. Measures and Trends U.S. and U.S.S.R. Strategic Force Effectiveness. (Sanitized)

    Science.gov (United States)

    1978-07-01

    the Anti-Submarine Warfare (AStiIW)rl,-, ip ± ,nse On thv avala4er if all sularines were on station, j each suhmarine would have over 150,000 squate ...V. Carter AIVA: fChIR of N!feft-tI:, .ta,6 uia KK ____~~~~~E~~~ SPC (con Ai*PW~ : ~ rfj tined) caal 40.teriai Ccrrdnd Oep.~ty ::.’ _~ Siff Departvtri

  16. Multisite Evaluation of APEX for Water Quality: I. Best Professional Judgment Parameterization.

    Science.gov (United States)

    Baffaut, Claire; Nelson, Nathan O; Lory, John A; Senaviratne, G M M M Anomaa; Bhandari, Ammar B; Udawatta, Ranjith P; Sweeney, Daniel W; Helmers, Matt J; Van Liew, Mike W; Mallarino, Antonio P; Wortmann, Charles S

    2017-11-01

    The Agricultural Policy Environmental eXtender (APEX) model is capable of estimating edge-of-field water, nutrient, and sediment transport and is used to assess the environmental impacts of management practices. The current practice is to fully calibrate the model for each site simulation, a task that requires resources and data not always available. The objective of this study was to compare model performance for flow, sediment, and phosphorus transport under two parameterization schemes: a best professional judgment (BPJ) parameterization based on readily available data and a fully calibrated parameterization based on site-specific soil, weather, event flow, and water quality data. The analysis was conducted using 12 datasets at four locations representing poorly drained soils and row-crop production under different tillage systems. Model performance was based on the Nash-Sutcliffe efficiency (NSE), the coefficient of determination () and the regression slope between simulated and measured annualized loads across all site years. Although the BPJ model performance for flow was acceptable (NSE = 0.7) at the annual time step, calibration improved it (NSE = 0.9). Acceptable simulation of sediment and total phosphorus transport (NSE = 0.5 and 0.9, respectively) was obtained only after full calibration at each site. Given the unacceptable performance of the BPJ approach, uncalibrated use of APEX for planning or management purposes may be misleading. Model calibration with water quality data prior to using APEX for simulating sediment and total phosphorus loss is essential. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.

  17. Hvorfor går Liberal Alliance frem?

    DEFF Research Database (Denmark)

    Kurrild-Klitgaard, Peter

    2010-01-01

    Liberal Alliance lå for kun lidt over et år siden ofte til 0 pct. i meningsmålingerne, men har siden haft et bemærkelsesværdigt comeback uden lige i nyere dansk politisk historie. Først fik man trukket sig over den symbolske »spærregrænse« på 2,0 pct., og i den seneste meningsmåling (fra You...

  18. Hvorfor går Liberal Alliance frem?

    DEFF Research Database (Denmark)

    Kurrild-Klitgaard, Peter

    2010-01-01

    Liberal Alliance lå for kun lidt over et år siden ofte til 0 pct. i meningsmålingerne, men har siden haft et bemærkelsesværdigt comeback uden lige i nyere dansk politisk historie. Først fik man trukket sig over den symbolske »spærregrænse« på 2,0 pct., og i den seneste meningsmåling ( fra You...

  19. Sub-lethal Ocular Trauma (SLOT): Establishing a Standardized Blast Threshold to Facilitate Diagnostic, Early Treatment, and Recovery Studies for Blast Injuries to the Eye and Optic Nerve

    Science.gov (United States)

    2014-09-01

    NSE (neuron specific enolase) PARK5/UCHL1 ( Parkinson Disease Protein 5) PARK7/DJ-1 ( Parkinson Disease Protein 7) 2. Human TH17 Magnetic Bead...for discovery of trauma-related biomarkers. 42 Figure 29. The average protein mass spectrum (abundance vs m/z). The color boxes indicate the...spectrum obtained from the tissue section shown in Figure 30. Color boxes indicate m/z ratios of corresponding protein spatial distributions in Figure 30

  20. Rapid Isolation and Detection for RNA Biomarkers for TBI Diagnostics

    Science.gov (United States)

    2015-10-01

    cervical adenocarcinoma) were obtained. • Primers were designed and tested for brain-specific mRNA targets: FABP, GFAP, NSE, MBP, S100B...Both of these values are generally painful to compute by hand, but trivial to evaluate in MATLAB once the data matrix is established in the tool...RNA from blood, plasma, and other samples. 100%/12 cal mos Tsukasa Takahashi Graduate student researcher/Staff Research Associate – helped obtain

  1. The Achievement Ideology and Top-Down National Standardized Exam Policy in Indonesia: Voices from Local English Teachers

    Directory of Open Access Journals (Sweden)

    Amirul Mukminin

    2013-04-01

    Full Text Available The purpose of this qualitative inquiry was to explore and describe the perspectives of local English teachers on the National Standardized Exam [NSE] policy as a high-stakes exam to gauge student performance nationally at four senior high schools in Jambi City, Indonesia. The theoretical framework of the ethics of critique, justice, and care was used to guide this study. Data for this study are collected through a demographic background survey and in-depth interviews with fifteen local English teachers. The demographic data were analyzed descriptively. The interviews data were analyzed using within-case and cross-case displays and analyses. Two salient themes with their subthemes that emerged were (1 negative perspectives on the practices and accuracy of the NSE policy and (2 unintended consequences the NSE policy (curriculum and instruction, teaching and learning, teacher motivation, student motivation, less attention to non-tested disciplines, and widespread cheating. This study provides information for policy makers, school leaders, researchers, and teacher educators to understand how the policy is implemented at the school level. Policy implications are discussed

  2. Validade de indicadores de atividade física e comportamento sedentário da Pesquisa Nacional de Saúde do Escolar entre adolescentes do Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Letícia Ferreira Tavares

    2014-09-01

    Full Text Available Analisou-se a validade relativa dos indicadores de atividade física do questionário utilizado na Pesquisa Nacional de Saúde do Escolar (PeNSE entre adolescentes da cidade do Rio de Janeiro, Brasil. Foram estudados 174 alunos. Avaliaram-se os seguintes indicadores referentes ao tempo semanal acumulado com a prática de atividade física: ATIVO-300MIN (acumulou 300 minutos ou mais; ATIVO-150MIN (acumulou 150 minutos ou mais; INATIVO (não praticou atividade física. Também foram estudados os indicadores de comportamento sedentário referentes ao tempo diário despendido assistindo TV, jogando videogame e usando computador. Foram comparados os resultados do questionário e dos três recordatórios de 24 horas. Os resultados de ATIVO-300MIN, ATIVO-150MIN e INATIVO gerados pela PeNSE apresentaram alta acurácia. Esses indicadores tiveram melhor desempenho que os de comportamento sedentário, tanto em relação às estimativas de frequência quanto em relação à sensibilidade, especificidade e índice de acurácia. Os indicadores de atividade física da PeNSE apresentaram validade relativa satisfatória.

  3. Immunohistochemical localization of gastrin-releasing peptide, neuronal nitric oxide synthase and neurone-specific enolase in the uterus of the North American opossum, Didelphis virginiana.

    Science.gov (United States)

    Kumano, A; Sasaki, M; Budipitojo, T; Kitamura, N; Krause, W J; Yamada, J

    2005-08-01

    The present study has demonstrated the immunohistochemical localization of gastrin-releasing peptide (GRP), neuronal nitric oxide synthase (nNOS) and neurone-specific enolase (NSE) in the uterus of the North American opossum. Although the presence of GRP, nNOS and NSE has been reported recently in the uterus of eutherian species this is the first description of these peptides in a metatherian species. Metatherian mammals are of interest because in these species it is the prolonged lactation phase of development that is the period of primary reproductive investment rather than intrauterine development as is true of eutherian mammals. The opossum, like other marsupial species, has a very abbreviated gestation period which in Didelphis lasts only 12.5 days. GRP was localized in the cytoplasm of cells forming the surface lining epithelium and the glandular epithelium of the opossum endometrium late in pregnancy, at 11.5 days of gestation. Likewise, immunoreactivities of nNOS and NSE were found primarily within the epithelial cells of the endometrium at 11.5 days of gestation. As these peptides and enzymes appear primarily at the time of establishment of the yolk sac placenta (between day 10 and day 12.5 gestation), the present results strongly suggest that these factors may play a fundamental role in the placentation of the opossum.

  4. Neutron spin echo spectroscopy. Its application to the study of the dynamics of polymers in solution

    International Nuclear Information System (INIS)

    Papoular, Robert

    1992-06-01

    This work focuses on Neutron Spin Echo (NSE) spectroscopy and on the NSE spectrometer MESS, which we have built at the L.L.B. (CE Saclay). After analyzing in detail the classical and quantum principles of this type of instrument, and illustrated them with optical analogies, we expound a simple formalism for the interpretation of polarized neutron experiments of the most general type. In a second part, we describe the MESS spectrometer extensively; its characteristics and performances as well as the first results obtained with this instrument. In particular, we include two papers showing how the neutron depolarization, spin rotation and echoes can be used to investigate high-Tc superconductors. The last part deals with the dynamics of Polymer-Polymer-Solvent ternary solutions and demonstrates how the Neutron Spin Echo technique becomes a privileged tool for such physico-chemical studies thanks to the joint use of NSE and contrast variation methods, coupled with the adequate ranges of time and scattering vectors accessible. Finally, we describe the specific case of partially deuterated polydimethyl-siloxane (PDMS) in semi-dilute solution in Toluene. We have experimentally and separately evidenced the cooperative and inter-diffusive diffusion modes predicted by the theory of Akcasu, Benoit, Benmouna et al. These results, obtained at the L.L.B. (CE Saclay) are the subject matter of the last paper included in this work. (author) [fr

  5. The effect of 3 different exercise approaches on neck muscle endurance, kinesiophobia, exercise compliance, and patient satisfaction in chronic whiplash.

    Science.gov (United States)

    Peterson, Gunnel E; Landén Ludvigsson, Maria H; O'Leary, Shaun P; Dedering, Åsa M; Wallman, Thorne; Jönsson, Margaretha I N; Peolsson, Anneli L C

    2015-09-01

    The purpose of this study was to compare the effects of 3 different exercise approaches on neck muscle endurance (NME), kinesiophobia, exercise compliance, and patient satisfaction in patients with chronic whiplash. This prospective randomized clinical trial included 216 individuals with chronic whiplash. Participants were randomized to 1 of 3 exercise interventions: neck-specific exercise (NSE), NSE combined with a behavioral approach (NSEB), or prescribed physical activity (PPA). Measures of ventral and dorsal NME (endurance time in seconds), perceived pain after NME testing, kinesiophobia, exercise compliance, and patient satisfaction were recorded at baseline and at the 3- and 6-month follow-ups. Compared with individuals in the prescribed physical activity group, participants in the NSE and NSEB groups exhibited greater gains in dorsal NME (P = .003), greater reductions in pain after NME testing (P = .03), and more satisfaction with treatment (P .07). Among patients with chronic whiplash, a neck-specific exercise intervention (with or without a behavioral approach) appears to improve NME. Participants were more satisfied with intervention including neck-specific exercises than with the prescription of general exercise. Copyright © 2015 National University of Health Sciences. Published by Elsevier Inc. All rights reserved.

  6. Neutron spin echo investigation of elementary excitations in superfluid 4He

    International Nuclear Information System (INIS)

    Mezei, F.

    1980-01-01

    The present work represents the first experimental evidence for the application of Neutron Spin Echo (NSE) in high resolution study of both optical-like (non dispersive) and dispersive elementary excitations. The results obtained proved to be relevant contributions concerning the temperature dependence of the energy and linewidth of the roton excitation between 0.96 and 1.4 K; the temperature dependence of the linewidth of the 1.1 A -1 and 1.72 A -1 phonons and the suggested onset of three-phonon decay between 2.1 A -1 and 2.4 A -1 . The energy transfer resolution achieved in this work was 10-40 times superiour to those in previous similar neutron scattering experiments. In this paper most of the attention will be paid to the experimental aspects. In the first section the details of the NSE experiment are described, with particular emphasis on the first demonstration of the general scheme of NSE focussing, which involves the tuning of both the ratio of the precession fields H 0 /H 1 and their geometrical assymetry ('tilt angle'). The second section gives the experimental results without, however, a detailed discussion of their significance for the understanding of superfluid 4 He, which will be published elsewhere. (orig.)

  7. An Improved Zero Potential Circuit for Readout of a Two-Dimensional Resistive Sensor Array

    Directory of Open Access Journals (Sweden)

    Jian-Feng Wu

    2016-12-01

    Full Text Available With one operational amplifier (op-amp in negative feedback, the traditional zero potential circuit could access one element in the two-dimensional (2-D resistive sensor array with the shared row-column fashion but it suffered from the crosstalk problem for the non-scanned elements’ bypass currents, which were injected into array’s non-scanned electrodes from zero potential. Firstly, for suppressing the crosstalk problem, we designed a novel improved zero potential circuit with one more op-amp in negative feedback to sample the total bypass current and calculate the precision resistance of the element being tested (EBT with it. The improved setting non-scanned-electrode zero potential circuit (S-NSE-ZPC was given as an example for analyzing and verifying the performance of the improved zero potential circuit. Secondly, in the S-NSE-ZPC and the improved S-NSE-ZPC, the effects of different parameters of the resistive sensor arrays and their readout circuits on the EBT’s measurement accuracy were simulated with the NI Multisim 12. Thirdly, part features of the improved circuit were verified with the experiments of a prototype circuit. Followed, the results were discussed and the conclusions were given. The experiment results show that the improved circuit, though it requires one more op-amp, one more resistor and one more sampling channel, can access the EBT in the 2-D resistive sensor array more accurately.

  8. An Improved Zero Potential Circuit for Readout of a Two-Dimensional Resistive Sensor Array.

    Science.gov (United States)

    Wu, Jian-Feng; Wang, Feng; Wang, Qi; Li, Jian-Qing; Song, Ai-Guo

    2016-12-06

    With one operational amplifier (op-amp) in negative feedback, the traditional zero potential circuit could access one element in the two-dimensional (2-D) resistive sensor array with the shared row-column fashion but it suffered from the crosstalk problem for the non-scanned elements' bypass currents, which were injected into array's non-scanned electrodes from zero potential. Firstly, for suppressing the crosstalk problem, we designed a novel improved zero potential circuit with one more op-amp in negative feedback to sample the total bypass current and calculate the precision resistance of the element being tested (EBT) with it. The improved setting non-scanned-electrode zero potential circuit (S-NSE-ZPC) was given as an example for analyzing and verifying the performance of the improved zero potential circuit. Secondly, in the S-NSE-ZPC and the improved S-NSE-ZPC, the effects of different parameters of the resistive sensor arrays and their readout circuits on the EBT's measurement accuracy were simulated with the NI Multisim 12. Thirdly, part features of the improved circuit were verified with the experiments of a prototype circuit. Followed, the results were discussed and the conclusions were given. The experiment results show that the improved circuit, though it requires one more op-amp, one more resistor and one more sampling channel, can access the EBT in the 2-D resistive sensor array more accurately.

  9. Development of seasonal flow outlook model for Ganges-Brahmaputra Basins in Bangladesh

    Science.gov (United States)

    Hossain, Sazzad; Haque Khan, Raihanul; Gautum, Dilip Kumar; Karmaker, Ripon; Hossain, Amirul

    2016-10-01

    Bangladesh is crisscrossed by the branches and tributaries of three main river systems, the Ganges, Bramaputra and Meghna (GBM). The temporal variation of water availability of those rivers has an impact on the different water usages such as irrigation, urban water supply, hydropower generation, navigation etc. Thus, seasonal flow outlook can play important role in various aspects of water management. The Flood Forecasting and Warning Center (FFWC) in Bangladesh provides short term and medium term flood forecast, and there is a wide demand from end-users about seasonal flow outlook for agricultural purposes. The objective of this study is to develop a seasonal flow outlook model in Bangladesh based on rainfall forecast. It uses European Centre for Medium-Range Weather Forecasts (ECMWF) seasonal precipitation, temperature forecast to simulate HYDROMAD hydrological model. Present study is limited for Ganges and Brahmaputra River Basins. ARIMA correction is applied to correct the model error. The performance of the model is evaluated using coefficient of determination (R2) and Nash-Sutcliffe Efficiency (NSE). The model result shows good performance with R2 value of 0.78 and NSE of 0.61 for the Brahmaputra River Basin, and R2 value of 0.72 and NSE of 0.59 for the Ganges River Basin for the period of May to July 2015. The result of the study indicates strong potential to make seasonal outlook to be operationalized.

  10. SWAT application in intensive irrigation systems: Model modification, calibration and validation

    Science.gov (United States)

    Dechmi, Farida; Burguete, Javier; Skhiri, Ahmed

    2012-11-01

    SummaryThe Soil and Water Assessment Tool (SWAT) is a well established, distributed, eco-hydrologic model. However, using the study case of an agricultural intensive irrigated watershed, it was shown that all the model versions are not able to appropriately reproduce the total streamflow in such system when the irrigation source is outside the watershed. The objective of this study was to modify the SWAT2005 version for correctly simulating the main hydrological processes. Crop yield, total streamflow, total suspended sediment (TSS) losses and phosphorus load calibration and validation were performed using field survey information and water quantity and quality data recorded during 2008 and 2009 years in Del Reguero irrigated watershed in Spain. The goodness of the calibration and validation results was assessed using five statistical measures, including the Nash-Sutcliffe efficiency (NSE). Results indicated that the average annual crop yield and actual evapotranspiration estimations were quite satisfactory. On a monthly basis, the values of NSE were 0.90 (calibration) and 0.80 (validation) indicating that the modified model could reproduce accurately the observed streamflow. The TSS losses were also satisfactorily estimated (NSE = 0.72 and 0.52 for the calibration and validation steps). The monthly temporal patterns and all the statistical parameters indicated that the modified SWAT-IRRIG model adequately predicted the total phosphorus (TP) loading. Therefore, the model could be used to assess the impacts of different best management practices on nonpoint phosphorus losses in irrigated systems.

  11. Biological Variation and Reference Change Value Data for Serum Neuron-Specific Enolase in a Turkish Population.

    Science.gov (United States)

    Matyar, Selcuk; Goruroglu Ozturk, Ozlem; Ziyanoglu Karacor, Esin; Yuzbasioglu Ariyurek, Sedefgul; Sahin, Gulhan; Kibar, Filiz; Yaman, Akgun; Inal, Tamer

    2016-11-01

    Neuron-specific enolase (NSE) is a recognized biomarker for the assessment of cerebral injury in neurological disorders. This study aims to report a definitive assessment of the biological variation (BV) components of this biomarker, including within-subject BV (CVI), between-subject BV (CVG), index of individuality (II), and reference change value (RCV), in a cohort of Turkish participants using an experimental protocol. Six blood specimens were collected from each of the 13 apparently healthy volunteers (seven women, six men; ranging in age from 23 to 36) on the same day, every 2 weeks for 2 months. Serum specimens were stored at -20°C until analysis. Neuron-specific enolase levels were evaluated in serum samples using an electrochemiluminescence (ECLIA) immunoassay kit with a Roche Cobas e 411 auto-analyser. ANOVA test was used to calculate the variations. The CVI and CVG for NSE were 21.5% and 28.8%, respectively. Analytical variation (CVA) was calculated as 10.2%. Additionally, II and RCV were calculated as 0.74 and 66% (95% confident interval, CI), respectively. As the performance index (PI) was found to be less than 2 (PI = 0.95), it is concluded that the NSE measurements have a desirable performance for analytical imprecision. Since the II was found to be less than 1 (II: 0.74), the reference values will be of little use. Thus, RCV would provide better information for deciding whether a significant change has occurred. © 2016 Wiley Periodicals, Inc.

  12. Correlation of serum homocysteine levels with nerve injury and atherosclerosis in patients with stroke

    Directory of Open Access Journals (Sweden)

    Gai-Zhuang Liu

    2017-07-01

    Full Text Available Objective: To study the correlation of serum homocysteine levels with nerve injury and atherosclerosis in patients with stroke. Methods: Patients who were diagnosed with ischemic stroke in our hospital between January 2014 and December 2016 were selected and then divided into moderate-severe stenosis group (C group, mild stenosis group (B group and no stenosis group (A group according to carotid artery ultrasonography; healthy volunteers who received physical examination during the same period were chosen as control group. The serum levels of homocysteine, nerve injury indexes and atherosclerosis indexes were detected. Results: Serum Hcy, S100B, NSE, UCH-L1, GFAP, FGF23, CD36, ox-LDL, MMP8 and MMP9 levels of C group, B group and A group were significantly higher than those of control group, and the severer the carotid stenosis, the higher the serum S100B, NSE, UCHL1, GFAP, FGF23, CD36, ox-LDL, MMP8 and MMP9 levels; serum S100B, NSE, UCHL1, GFAP, FGF23, CD36, ox-LDL, MMP8 and MMP9 levels in stoke patients with high Hcy were significantly higher than those of patients with normal Hcy. Conclusions: Serum homocysteine levels increase in patients with stroke and are closely related to the nerve injury and atherosclerosis.

  13. A neutron spin echo spectrometer with two optimal field shape coils for neutron spin precession

    International Nuclear Information System (INIS)

    Takeda, T.; Ebisawa, T.; Tasaki, S.; Ito, Y.; Takahashi, S.; Yoshizawa, H.

    1995-01-01

    We have designed and have been constructing at the C 2-2 cold neutron guide port of JRR-3M, JAERI, a neutron spin echo spectrometer (NSE) which is equipped with two optimal field shape (OFS) coils for neutron spin precession with the maximum field integral of 0.22 T m, an assembly of position sensitive detectors (PSD), a converging polarizer and a wide area analyzer. The dynamic range of scattering vector Q covers from 0.005 A -1 to 0.2 A -1 and that of energy hω from 10 neV to 30 μeV. Performance tests of the OFS coils show that the inhomogeneity of the magnetic field integral in the OFS coils with the spiral coils is so small that the NSE signal amplitude decreases little even for the neutron cross section of 30 mm diameter as the Fourier time t increases up to 25 ns, though the precession coils are close to iron covers of the neighboring neutron guide. This verifies that the OFS precession coils are appropriate for this NSE spectrometer. Another test experiment shows that the homogeneity condition of the precession magnet is loosened by use of PSD. (orig.)

  14. Weak Form Efficiency of the Insurance Industry: Empirical Evidence from Nigeria

    Directory of Open Access Journals (Sweden)

    Emenike Kalu Onwukwe

    2018-02-01

    Full Text Available This paper evaluated the insurance sector of Nigeria Stock Exchange (NSE for evidence weak-form efficiency using daily returns from January 2009 to February 2016. The study employs descriptive analysis, non-parametric runs test and autocorrelation function as well as Ljung-Box Q statistics in conducting the evaluation. Descriptive statistics of the insurance sector return series showed negative skewness and leptokurtic distribution. Estimates from the Jarque-Bera normality test showed that the insurance sector returns did not follow normal distribution. Results of the runs test reject null hypothesis of randomness in the return series of the insurance sector in the period studied. Furthermore, the autocorrelation functions and the Ljung-Box Q tests provide evidence of serial correlation in the stock returns of the insurance sector. Overall results from the study suggested that the insurance sector of NSE is not weak-form efficient. Consequently, technical analysis on the insurance sector of the NSE may not be fruitless.

  15. Spin interference of neutrons tunneling through magnetic thin films

    International Nuclear Information System (INIS)

    Hino, Masahiro; Achiwa, Norio; Tasaki, Seiji; Ebisawa, Toru; Akiyoshi, Tsunekazu; Kawai, Takeshi.

    1996-01-01

    Larmor precession of a neutron spin is represented as the superposition of the wave functions of the two Stern-Gerlach states ↑ and ↓. A transverse neutron spin echo (NSE) spectrometer can hence be used as a neutron spin interferometer (NSI) by setting a magnetic film, such as iron and permalloy45 (Fe 55 Ni 45 ), thin enough to permit tunneling at an incident angle above and below the critical angle of the total reflection in the Larmor precession field. The NSI can be used to study spin coherent superposition and rotation of the Larmor precession through a magnetic thin film for a tunneling ↑ spin neutron and a non-tunneling ↓ spin neutron and to get the tunneling time using Larmor clock. The NSI experiments were carried out to measure the shifts of NSE signals transmitted through magnetic iron films with thicknesses of 200 and 400 A and those magnetic permalloy45 films with thicknesses of 200 and 400 A, respectively, as a function of the incident angle. Then even in tunneling ↑ spin neutron and non-tunneling ↓ spin neutron, NSE signal was observed. The phase delay was measured in iron and permalloy45 films with thickness of 200 A, and the tunneling time using Larmor clock was estimated to be 4 ± 0.6 x 10 -9 sec. (author)

  16. Neuroimmunological Disturbance Features in Premature Infants with Perinatal Infections

    Directory of Open Access Journals (Sweden)

    Nailya J. Rahimova

    2018-01-01

    Full Text Available Infectious diseases in newborns are commonly intrauterine infections which affect greatly on the morbidity and mortality rates in neonates.Background: The purpose of this study was to analyse the neurological status, taking into account the neuroimmunological indicators (neuron-specific enolase (NSE, interleukin-1β (IL1β, Interleukin-6 (IL6 in the serum of neonates with perinatal infections.Metods: We conducted a complex clinical, laboratory, and instrumental examination of 433 infants with perinatal infections with a gestation period of 27–37 weeks. Determination of the level of NSE, IL1β, IL6 was performed with the standard method of the immune-enzyme analysis.Results. Hypoxic ischemic, hemorrhagic, infectious lesion of the central nervous system (CNS were more common in newborns with mixed infection and sepsis. High levels of NSE, IL6, IL1β in the serum of the examined newborns reflect a combined, deeper character of the CNS damage.Conclusion: Significant diagnostic value of neuroimmunological indicators in the blood serum of newborns with perinatal infections makes it possible to use them as a markers for assessing the severity of the CNS lesions.

  17. Fertility in Gyr Cows (Bos indicus with Fixed Time Artificial Insemination and Visual Estrus Detection Using a Classification Table

    Directory of Open Access Journals (Sweden)

    Lilido Nelson Ramírez-Iglesia

    2014-01-01

    Full Text Available The aim of this research was to compare two artificial insemination protocols (AIP: hormonal synchronization with fixed time artificial insemination (SC-FTAI and the use of a table based on visual observation of estrus signs (VO in order to identify cows in natural or spontaneous estrus being assigned to AI (NSE-IA. Two groups were formed: in the first group 109 cows were assigned to SC-FTAI, in which a commercial protocol is used; the second one included 108 randomly chosen cows, which were assigned to NSE-AI and in this group a modified table was used. Response variable was first service fertility rate (FSF, which was coded 1 for pregnant and 0 for empty. Predictor variables were AIP, postpartum anestrus, daily milk yield, body condition score at AI and calving number. Statistical analyses included association chi-square tests and logistic regression. Results showed an overall 41.94% FSF and a significant association was detected (P0.05. The odds ratio for the effect of AIP was only 1.050, suggesting no differences in FSF between groups. The NSE-AI protocol can enhance both the technique of VO and reproductive efficiency. Further validation of the table is required.

  18. Influence of intracerebral exposure to enriched uranium on neutron specific enolase and interleukin-1 β content in neonatal rats

    International Nuclear Information System (INIS)

    Gu Guixiong; Zhu Shoupeng; Wang Liuyi; Yang Shuqin; Zhu Lingli

    2001-01-01

    Objective: To examine biochemically the injurious effects of enriched uranium 235 U on developing brain of neonatal rats. Methods: Neonatal rats were irradiated with single injection of 2 μl enriched uranium into the left lateral ventricle of the brain at postnatal day 1 ( 235 U, respectively. The micro-autoradiographic tracing was performed, somatic growth and neuro-behavior development of neonatal rats were examined by determination of multiple parameters, and the neuron specific enolase (NSE) and interleukin-1 β(IL-1 β) levels in brains were determined with radioimmunoassay. Results: The radionuclides were mainly accumulated in the neuronal nucleus, and autoradiographic tracks appeared in the cytoplasm and inter- cellular space. Neonatal rats showed delayed growth and abnormal neuro-behavior. The changes of NSE, IL-1 β in cerebellum, cerebral cortex, hippocampus, diencephalons showed a dose-dependent relationship that when the dose of irradiation was increased, the levels of NSE was decreased and the IL-1 β was increased. Conclusion: The nerve cell of developing brain of neonatal rats is sensitive, fragile and compensable to injurious effects of α-irradiation from enriched uranium

  19. Incremental learning of concept drift in nonstationary environments.

    Science.gov (United States)

    Elwell, Ryan; Polikar, Robi

    2011-10-01

    We introduce an ensemble of classifiers-based approach for incremental learning of concept drift, characterized by nonstationary environments (NSEs), where the underlying data distributions change over time. The proposed algorithm, named Learn(++). NSE, learns from consecutive batches of data without making any assumptions on the nature or rate of drift; it can learn from such environments that experience constant or variable rate of drift, addition or deletion of concept classes, as well as cyclical drift. The algorithm learns incrementally, as other members of the Learn(++) family of algorithms, that is, without requiring access to previously seen data. Learn(++). NSE trains one new classifier for each batch of data it receives, and combines these classifiers using a dynamically weighted majority voting. The novelty of the approach is in determining the voting weights, based on each classifier's time-adjusted accuracy on current and past environments. This approach allows the algorithm to recognize, and act accordingly, to the changes in underlying data distributions, as well as to a possible reoccurrence of an earlier distribution. We evaluate the algorithm on several synthetic datasets designed to simulate a variety of nonstationary environments, as well as a real-world weather prediction dataset. Comparisons with several other approaches are also included. Results indicate that Learn(++). NSE can track the changing environments very closely, regardless of the type of concept drift. To allow future use, comparison and benchmarking by interested researchers, we also release our data used in this paper. © 2011 IEEE

  20. Patent citation network in nanotechnology (1976-2004)

    International Nuclear Information System (INIS)

    Li Xin; Chen Hsinchun; Huang Zan; Roco, Mihail C.

    2007-01-01

    The patent citation networks are described using critical node, core network, and network topological analysis. The main objective is understanding of the knowledge transfer processes between technical fields, institutions and countries. This includes identifying key influential players and subfields, the knowledge transfer patterns among them, and the overall knowledge transfer efficiency. The proposed framework is applied to the field of nanoscale science and engineering (NSE), including the citation networks of patent documents, submitting institutions, technology fields, and countries. The NSE patents were identified by keywords 'full-text' searching of patents at the United States Patent and Trademark Office (USPTO). The analysis shows that the United States is the most important citation center in NSE research. The institution citation network illustrates a more efficient knowledge transfer between institutions than a random network. The country citation network displays a knowledge transfer capability as efficient as a random network. The technology field citation network and the patent document citation network exhibit a less efficient knowledge diffusion capability than a random network. All four citation networks show a tendency to form local citation clusters

  1. Weak-form Efficiency of the Insurance Industry: Empirical Evidence from Nigeria

    Directory of Open Access Journals (Sweden)

    Emenike Kalu Onwukwe

    2018-02-01

    Full Text Available This paper evaluates the insurance sector of Nigeria Stock Exchange (NSE for evidence weak-form efficiency using daily returns from January 2009 to February 2016. The study employs descriptive analysis, non-parametric runs test and autocorrelation function as well as Ljung-Box Q statistics in conducting the evaluation. Descriptive statistics of the insurance sector return series show negative skewness and leptokurtic distribution. Estimates from the Jarque-Bera normality test show that the insurance sector returns do not follow a normal distribution. Results of the runs test reject the null hypothesis of randomness in the return series of the insurance sector in the period studied. Furthermore, the autocorrelation functions and the Ljung-Box Q tests provide evidence of serial correlation in the stock returns of the insurance sector. Overall results from the study suggest that the insurance sector of NSE is not weak-form efficient. Consequently, technical analysis on the insurance sector of the NSE may not be fruitless.

  2. On the dynamics of polymers in dense systems - Results of neutron spin echo spectroscopy

    International Nuclear Information System (INIS)

    Richter, D.

    1997-01-01

    One of the basic problems in the dynamics of polymers concerns the importance of geometrical or topological interactions which are directly related to the large scale molecular structures. In the famous reptation model these constraints are pictured in terms of a tube of localization following the average chain profile and confining the chain motion to the curve-linear tube. Recently studying the dynamic structure factor of a single labeled chain in a polymer melt by means of neutron spin echo spectroscopy (NSE) led to a direct observation of these tube constraints. Here I shall summarize these neutron spin echo experiments. I shall address the NSE technique, present results on the entropy driven segmental chain dynamics, discuss the dynamics of single chains in the melt where the chain length is increased through the transition to 'reptation' dynamics and display NSE measurements on long chain systems which revealed the molecular existence of the entanglement distance. Their magnitudes agree very well with tube diameters derived from dynamical mechanical measurements on the basis of the reptation model proving thereby the basic assumption of this Nobel Price winning concept

  3. Experiencia con Símbolos y Comprensión de Dibujos en Niños Pequeños de Distintos Contextos Socioeconómicos

    Directory of Open Access Journals (Sweden)

    Analía M. Salsa

    2018-01-01

    Full Text Available El propósito de esta investigación fue explorar las relaciones entre la comprensión temprana de dibujos por parte de niños de nivel socioeconómico medio (nse medio y bajo (nse bajo y las experiencias con símbolos en el contexto familiar. Para ello, 28 niños de entre 30 y 38 meses resolvieron una tarea de correspondencias en la que debían establecer relaciones entre cinco objetos y sus dibujos en lápiz negro. Sus madres contestaron un cuestionario sobre la cantidad y la variedad de imá- genes en los hogares y las actividades compartidas por adultos y niños con estas representaciones. Los resultados muestran que solamente los niños de nse medio demostraron comprender la relación dibujo-referente; el desempeño simbólico se asoció principalmente con la presencia en los hogares de recursos con imágenes para niños (dvd y libros infantiles y con las interacciones adulto-niño con estos recursos, como las experiencias conjuntas con imágenes audiovisuales, la lectura de cuentos y la asistencia materna al dibujar

  4. The impact of enhancing perceived self-efficacy in torture survivors.

    Science.gov (United States)

    Morina, Naser; Bryant, Richard A; Doolan, Emma L; Martin-Sölch, Chantal; Plichta, Michael M; Pfaltz, Monique C; Schnyder, Ulrich; Schick, Matthis; Nickerson, Angela

    2018-01-01

    Perceived self-efficacy (SE) is an important factor underlying psychological well-being. Refugees suffer many experiences that can compromise SE. This study tested the impact of enhancing perceived SE on coping with trauma reminders and distress tolerance in tortured refugees. Torture survivors (N = 40) were administered a positive SE induction in which they retrieved mastery-related autobiographical memories, or a non-SE (NSE) induction, and then viewed trauma-related images. Participants rated their distress following presentation of each image. Participants then completed a frustration-inducing mirror-tracing task to index distress tolerance. Participants in the SE condition reported less distress and negative affect, and improved coping in relation to viewing the trauma-related images than those in the NSE condition. The SE induction also led to greater persistence with the mirror-tracing task than the NSE induction. These findings provide initial evidence that promoting SE in tortured refugees can assist with managing distress from trauma reminders, and promoting greater distress tolerance. Enhancing perceived SE in tortured refugees may increase their capacity to tolerate distress during therapy, and may be a useful means to improve treatment response. © 2017 Wiley Periodicals, Inc.

  5. Predicting Hydrologic Function With Aquatic Gene Fragments

    Science.gov (United States)

    Good, S. P.; URycki, D. R.; Crump, B. C.

    2018-03-01

    Recent advances in microbiology techniques, such as genetic sequencing, allow for rapid and cost-effective collection of large quantities of genetic information carried within water samples. Here we posit that the unique composition of aquatic DNA material within a water sample contains relevant information about hydrologic function at multiple temporal scales. In this study, machine learning was used to develop discharge prediction models trained on the relative abundance of bacterial taxa classified into operational taxonomic units (OTUs) based on 16S rRNA gene sequences from six large arctic rivers. We term this approach "genohydrology," and show that OTU relative abundances can be used to predict river discharge at monthly and longer timescales. Based on a single DNA sample from each river, the average Nash-Sutcliffe efficiency (NSE) for predicted mean monthly discharge values throughout the year was 0.84, while the NSE for predicted discharge values across different return intervals was 0.67. These are considerable improvements over predictions based only on the area-scaled mean specific discharge of five similar rivers, which had average NSE values of 0.64 and -0.32 for seasonal and recurrence interval discharge values, respectively. The genohydrology approach demonstrates that genetic diversity within the aquatic microbiome is a large and underutilized data resource with benefits for prediction of hydrologic function.

  6. A new application of scanning electrochemical microscopy for the label-free interrogation of antibody-antigen interactions

    Energy Technology Data Exchange (ETDEWEB)

    Holmes, Joanne L.; Davis, Frank; Collyer, Stuart D. [Cranfield Health, Cranfield University, Cranfield, MK43 0AL (United Kingdom); Higson, Seamus P.J., E-mail: s.p.j.higson@cranfield.ac.uk [Cranfield Health, Cranfield University, Cranfield, MK43 0AL (United Kingdom)

    2011-03-18

    Within this work we present a 'proof of principle' study for the use of scanning electrochemical microscopy (SECM) to detect and image biomolecular interactions in a label-free assay as a potential alternative to current fluorescence techniques. Screen-printed carbon electrodes were used as the substrate for the deposition of a dotted array, where the dots consist of biotinylated polyethyleneimine. These were then further derivatised, first with neutravidin and then with a biotinylated antibody to the protein neuron specific enolase (NSE). SECM using a ferrocene carboxylic acid mediator showed clear differences between the array and the surrounding unmodified carbon. Imaging of the arrays before and following exposure to various concentrations of the antigen showed clear evidence for specific binding of the NSE antigen to the antibody derivatised dots. Non-specific binding was quantified. Control experiments with other proteins showed only non-specific binding across the whole of the substrate, thereby confirming that specific binding does occur between the antibody and antigen at the surface of the dots. Binding of the antigen was accompanied by a measured increase in current response, which may be explained in terms of protein electrostatic interaction and hydrophobic interactions to the mediator, thereby increasing the localised mediator flux. A calibration curve was obtained between 500 fg mL{sup -1} to 200 pg mL{sup -1} NSE which demonstrated a logarithmic relationship between the current change upon binding and antigen concentration without the need for any labelling of the substrate.

  7. CSF and Serum Biomarkers Focusing on Cerebral Vasospasm and Ischemia after Subarachnoid Hemorrhage

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    Carla S. Jung

    2013-01-01

    Full Text Available Delayed cerebral vasospasm (CVS and delayed cerebral ischemia (DCI remain severe complications after subarachnoid hemorrhage (SAH. Although focal changes in cerebral metabolism indicating ischemia are detectable by microdialysis, routinely used biomarkers are missing. We therefore sought to evaluate a panel of possible global markers in serum and cerebrospinal fluid (CSF of patients after SAH. CSF and serum of SAH patients were analyzed retrospectively. In CSF, levels of inhibitory, excitatory, and structural amino acids were detected by high-performance liquid chromatography (HPLC. In serum, neuron-specific enolase (NSE and S100B level were measured and examined in conjunction with CVS and DCI. CVS was detected by arteriography, and ischemic lesions were assessed by computed tomography (CT scans. All CSF amino acids were altered after SAH. CSF glutamate, glutamine, glycine, and histidine were significantly correlated with arteriographic CVS. CSF glutamate and serum S100B were significantly correlated with ischemic events after SAH; however, NSE did not correlate neither with ischemia nor with vasospasm. Glutamate, glutamine, glycine, and histidine might be used in CSF as markers for CVS. Glutamate also indicates ischemia. Serum S100B, but not NSE, is a suitable marker for ischemia. These results need to be validated in larger prospective cohorts.

  8. Inflammation-induced reversible switch of the neuron-specific enolase promoter from Purkinje neurons to Bergmann glia.

    Science.gov (United States)

    Sawada, Yusuke; Konno, Ayumu; Nagaoka, Jun; Hirai, Hirokazu

    2016-06-13

    Neuron-specific enolase (NSE) is a glycolytic isoenzyme found in mature neurons and cells of neuronal origin. Injecting adeno-associated virus serotype 9 (AAV9) vectors carrying the NSE promoter into the cerebellar cortex is likely to cause the specific transduction of neuronal cells, such as Purkinje cells (PCs) and interneurons, but not Bergmann glia (BG). However, we found BG-predominant transduction without PC transduction along a traumatic needle tract for viral injection. The enhancement of neuroinflammation by the co-application of lipopolysaccharide (LPS) with AAV9 significantly expanded the BG-predominant area concurrently with the potentiated microglial activation. The BG-predominant transduction was gradually replaced by the PC-predominant transduction as the neuroinflammation dissipated. Experiments using glioma cell cultures revealed significant activation of the NSE promoter due to glucose deprivation, suggesting that intracellularly stored glycogen is metabolized through the glycolytic pathway for energy. Activation of the glycolytic enzyme promoter in BG concurrently with inactivation in PC may have pathophysiological significance for the production of lactate in activated BG and the utilization of lactate, which is provided by the BG-PC lactate shuttle, as a primary energy resource in injured PCs.

  9. Reference Evapotranspiration Retrievals from a Mesoscale Model Based Weather Variables for Soil Moisture Deficit Estimation

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    Prashant K. Srivastava

    2017-10-01

    Full Text Available Reference Evapotranspiration (ETo and soil moisture deficit (SMD are vital for understanding the hydrological processes, particularly in the context of sustainable water use efficiency in the globe. Precise estimation of ETo and SMD are required for developing appropriate forecasting systems, in hydrological modeling and also in precision agriculture. In this study, the surface temperature downscaled from Weather Research and Forecasting (WRF model is used to estimate ETo using the boundary conditions that are provided by the European Center for Medium Range Weather Forecast (ECMWF. In order to understand the performance, the Hamon’s method is employed to estimate the ETo using the temperature from meteorological station and WRF derived variables. After estimating the ETo, a range of linear and non-linear models is utilized to retrieve SMD. The performance statistics such as RMSE, %Bias, and Nash Sutcliffe Efficiency (NSE indicates that the exponential model (RMSE = 0.226; %Bias = −0.077; NSE = 0.616 is efficient for SMD estimation by using the Observed ETo in comparison to the other linear and non-linear models (RMSE range = 0.019–0.667; %Bias range = 2.821–6.894; NSE = 0.013–0.419 used in this study. On the other hand, in the scenario where SMD is estimated using WRF downscaled meteorological variables based ETo, the linear model is found promising (RMSE = 0.017; %Bias = 5.280; NSE = 0.448 as compared to the non-linear models (RMSE range = 0.022–0.707; %Bias range = −0.207–−6.088; NSE range = 0.013–0.149. Our findings also suggest that all the models are performing better during the growing season (RMSE range = 0.024–0.025; %Bias range = −4.982–−3.431; r = 0.245–0.281 than the non−growing season (RMSE range = 0.011–0.12; %Bias range = 33.073–32.701; r = 0.161–0.244 for SMD estimation.

  10. The level of serum tumor makers and bone metastases of lung cancer correlation

    International Nuclear Information System (INIS)

    Li Li; Jin Jianhua

    2014-01-01

    Objective: To study the correlation between the level of serum tumor makers and bone metastases of lung cancer. Method: In 128 diagnosed patients with lung cancer, small cell lung cancer were 26 cases, non-small cell lung cancer were 102 cases which included 44 cases of adenocarcinoma, 50 cases of squamous cell carcinoma, 4 cases of large cell carcinoma, 4 cases of squamous adenocarcinoma. "9"9"mTc-MDP whole-body bone scanning was performed in 128 patients with lung cancer. over the same period, the serum samples were collected in these patients and 30 comparison controls. CEA, CA125, CA199, SCC, NSE, CA15-3, and AFP were measured by ELISA technique. Bone imaging findings analysis used t-test, and serum levels of tumor markers analysis used χ"2 test. Results: The diagnostic of 53 cases of lung cancer with bone metastasis was subject to clinical criteria of lung cancer with bone metastases. The positive ratio of patients with osseous metastasis was confirmed by "9"9"mTc-MDP whole-body bone scanning was 23.44% (30/128), including 16 cases of lung adenocarcinoma, 9 cases of squamous cell carcinoma, 3 cases of small cell lung cancer , 1 case of large cell lung cancer, 1 case of squamous adenocarcinoma and multiple bone metastases accounted for 66.67% (20/30). The levels of serum CEA, CA125, CA199, SCC, NSE and CA15-3 were higher than the control group (P < O.05). 29 cases of CEA positive and 21 cases of CA125 positive were included in 30 cases of lung cancer with bone metastasis. There was a significant difference between the levels of CEA, CA125, CA199, NSE in lung cancer with bone metastases and without bone metastases (P < 0.05). The sensitivity of "9"9"mTc-MDP whole-body bone scanning in diagnosis of lung cancer with bone metastasis was 84.91%. Conclusion: The average value of CEA, CA125, and CA199, SCC, NSE and CA15-3 in lung cancer patients were significantly higher than the control group. In addition, there is a significantly correlation between the occurrence

  11. Comparing Machine Learning and Decision Making Approaches to Forecast Long Lead Monthly Rainfall: The City of Vancouver, Canada

    Directory of Open Access Journals (Sweden)

    Zahra Zahmatkesh

    2018-01-01

    Full Text Available Estimating maximum possible rainfall is of great value for flood prediction and protection, particularly for regions, such as Canada, where urban and fluvial floods from extreme rainfalls have been known to be a major concern. In this study, a methodology is proposed to forecast real-time rainfall (with one month lead time using different number of spatial inputs with different orders of lags. For this purpose, two types of models are used. The first one is a machine learning data driven-based model, which uses a set of hydrologic variables as inputs, and the second one is an empirical-statistical model that employs the multi-criteria decision analysis method for rainfall forecasting. The data driven model is built based on Artificial Neural Networks (ANNs, while the developed multi-criteria decision analysis model uses Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS approach. A comprehensive set of spatially varying climate variables, including geopotential height, sea surface temperature, sea level pressure, humidity, temperature and pressure with different orders of lags is collected to form input vectors for the forecast models. Then, a feature selection method is employed to identify the most appropriate predictors. Two sets of results from the developed models, i.e., maximum daily rainfall in each month (RMAX and cumulative value of rainfall for each month (RCU, are considered as the target variables for forecast purpose. The results from both modeling approaches are compared using a number of evaluation criteria such as Nash-Sutcliffe Efficiency (NSE. The proposed models are applied for rainfall forecasting for a coastal area in Western Canada: Vancouver, British Columbia. Results indicate although data driven models such as ANNs work well for the simulation purpose, developed TOPSIS model considerably outperforms ANNs for the rainfall forecasting. ANNs show acceptable simulation performance during the

  12. Associations between neural injury markers of intrauterine growth-restricted infants and neurodevelopment at 2 years of age.

    Science.gov (United States)

    Mazarico, E; Llurba, E; Cabero, L; Sánchez, O; Valls, A; Martín-Ancel, A; Cardenas, D; Gómez Roig, M D

    2018-04-18

    The aim of this study was to evaluate the relationships between brain injury biomarkers in intrauterine growth-restricted (IUGR) infants (S100B and neuron-specific enolase (NSE)) and neurodevelopment at 2 years of age. This prospective case-control study was a cooperative effort among Spanish Maternal and Child Health Network (Retic SAMID) hospitals. At inclusion, biometry for estimated fetal weight and feto-placental Doppler variables were measured for each infant. Maternal venous blood and fetal umbilical arterial blood samples were collected at the time of delivery and neural injury markers S100B and NSE concentrations were measured. Neurodevelopment was evaluated at 2 years of age using the Bayley Scales of Infant and Toddler Development, third edition (Bayley-III). Fifty six pregnancies were included. Thirty-one infants were classified as IUGR and 25 as non-IUGR. Neurodevelopmental evaluation at 2 years of age indicated that there were no between-group differences for any of the tests. For all patients in both groups, we found statistically significant inverse relationships between the concentrations of NSE in the cord blood and the results of the cognitive test (r = -271, p = .042), fine motor subtest (r = -280, p = .036), and social-emotional test (r = -349, p = .015). We also found statistically significant differences between the concentrations of S100B in the cord blood and the results of the cognitive test (r = -306, p = .022) and expressive communication subtest (r = -304, p = .023). For the IUGR group, we found a significant inverse relationship between the concentrations of S100B in the maternal serum and the results of adaptive behavior test (p < .05). In the non-IUGR group, we found statistically significant inverse relationships between the concentration of NSE in the cord blood and the results of the fine motor subtest (r = -446, p = .025) and social-emotional test (r = -489, p = .021

  13. Calibration of Spatially Distributed Hydrological Processes and Model Parameters in SWAT Using Remote Sensing Data and an Auto-Calibration Procedure: A Case Study in a Vietnamese River Basin

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    Lan Thanh Ha

    2018-02-01

    Full Text Available In this paper, evapotranspiration (ET and leaf area index (LAI were used to calibrate the SWAT model, whereas remotely sensed precipitation and other climatic parameters were used as forcing data for the 6300 km2 Day Basin, a tributary of the Red River in Vietnam. The efficacy of the Sequential Uncertainty Fitting (SUFI-2 parameter sensitivity and optimization model was tested with area specific remote sensing input parameters for every Hydrological Response Units (HRU, rather than with measurements of river flow representing a large set of HRUs, i.e., a bulk calibration. Simulated monthly ET correlations with remote sensing estimates showed an R2 = 0.71, Nash–Sutcliffe Efficiency NSE = 0.65, and Kling Gupta Efficiency KGE = 0.80 while monthly LAI showed correlations of R2 = 0.59, NSE = 0.57 and KGE = 0.83 over a five-year validation period. Accumulated modelled ET over the 5-year calibration period amounted to 5713 mm compared to 6015 mm of remotely sensed ET, yielding a difference of 302 mm (5.3%. The monthly flow at two flow measurement stations were adequately estimated (R2 = 0.78 and 0.55, NSE = 0.71 and 0.63, KGE = 0.59 and 0.75 for Phu Ly and Ninh Binh, respectively. This outcome demonstrates the capability of SWAT model to obtain spatial and accurate simulation of eco-hydrological processes, also when rivers are ungauged and the water withdrawal system is complex.

  14. Night-time care routine interaction and sleep disruption in adult cardiac surgery.

    Science.gov (United States)

    Casida, Jesus M; Davis, Jean E; Zalewski, Aaron; Yang, James J

    2018-04-01

    To explore the context and the influence of night-time care routine interactions (NCRIs) on night-time sleep effectiveness (NSE) and daytime sleepiness (DSS) of patients in the cardiac surgery critical-care and progressive-care units of a hospital. There exists a paucity of empirical data regarding the influence of NCRIs on sleep and associated outcomes in hospitalised adult cardiac surgery patients. An exploratory repeated-measures research design was employed on the data provided by 38 elective cardiac surgery patients (mean age 60.0 ± 15.9 years). NCRI forms were completed by the bedside nurses and patients completed a 9-item Visual Analogue Sleep Scale (100-mm horizontal lines measuring NSE and DSS variables). All data were collected during postoperative nights/days (PON/POD) 1 through 5 and analysed with IBM SPSS software. Patient assessment, medication administration and laboratory/diagnostic procedures were the top three NCRIs reported between midnight and 6:00 a.m. During PON/POD 1 through 5, the respective mean NSE and DSS scores ranged from 52.9 ± 17.2 to 57.8 ± 13.5 and from 27.0 ± 22.6 to 45.6 ± 16.5. Repeated-measures ANOVA showed significant changes in DSS scores (p  .05). Finally, of 8 NCRIs, only 1 (postoperative exercises) was significantly related to sleep variables (r > .40, p disruptions and daytime sleepiness in adult cardiac surgery. Worldwide, acute and critical-care nurses are well positioned to lead initiatives aimed at improving sleep and clinical outcomes in cardiac surgery. © 2018 John Wiley & Sons Ltd.

  15. Improvement of Hydrological Simulations by Applying Daily Precipitation Interpolation Schemes in Meso-Scale Catchments

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    Mateusz Szcześniak

    2015-02-01

    Full Text Available Ground-based precipitation data are still the dominant input type for hydrological models. Spatial variability in precipitation can be represented by spatially interpolating gauge data using various techniques. In this study, the effect of daily precipitation interpolation methods on discharge simulations using the semi-distributed SWAT (Soil and Water Assessment Tool model over a 30-year period is examined. The study was carried out in 11 meso-scale (119–3935 km2 sub-catchments lying in the Sulejów reservoir catchment in central Poland. Four methods were tested: the default SWAT method (Def based on the Nearest Neighbour technique, Thiessen Polygons (TP, Inverse Distance Weighted (IDW and Ordinary Kriging (OK. =The evaluation of methods was performed using a semi-automated calibration program SUFI-2 (Sequential Uncertainty Fitting Procedure Version 2 with two objective functions: Nash-Sutcliffe Efficiency (NSE and the adjusted R2 coefficient (bR2. The results show that: (1 the most complex OK method outperformed other methods in terms of NSE; and (2 OK, IDW, and TP outperformed Def in terms of bR2. The median difference in daily/monthly NSE between OK and Def/TP/IDW calculated across all catchments ranged between 0.05 and 0.15, while the median difference between TP/IDW/OK and Def ranged between 0.05 and 0.07. The differences between pairs of interpolation methods were, however, spatially variable and a part of this variability was attributed to catchment properties: catchments characterised by low station density and low coefficient of variation of daily flows experienced more pronounced improvement resulting from using interpolation methods. Methods providing higher precipitation estimates often resulted in a better model performance. The implication from this study is that appropriate consideration of spatial precipitation variability (often neglected by model users that can be achieved using relatively simple interpolation methods can

  16. Efectos de la actividad física y del nivel socioeconómico en el sobrepeso y obesidad de escolares, Lima Este 2005

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    Alcibíades Bustamante V

    2007-04-01

    Full Text Available Objetivos: Documentar la prevalencia de sobrepeso y obesidad en escolares de 6 a 11 años de edad, y determinar la influencia del nivel socioeconómico y de la actividad física sobre ellos. Materiales y métodos: Se evaluaron 4191 niños de siete instituciones educativas de Lima Este. El sobrepeso y la obesidad fueron determinados a través del índice de masa corporal, los niveles de actividad física fueron evaluados a partir del cuestionario de Godin y Shephard y la caracterización del nivel socioeconómico (NSE fue según la escuela donde cursaban estudios. Resultados: La prevalencia de sobrepeso y obesidad fue de 22 y 7% respectivamente, 32,9% de los escolares fueron considerados como poco activos. Los escolares de NSE alto tienen mayor riesgo de presentar sobrepeso (OR: 3,9; IC95%: 3,1- 4,9 o ser obesos (OR: 11,5; IC95%: 7,3-18,2 independientemente de la edad, sexo y nivel de actividad física. No se encontró asociación entre los niveles de actividad física y el sobrepeso u obesidad. Conclusiones: La prevalencia de sobrepeso y obesidad que se encontró refuerza la preocupación mundial con la obesidad infantil y sus consecuencias en el futuro. Los factores más influyentes para el sobrepeso y la obesidad fueron el NSE, edad y sexo. La actividad física no presenta ninguna asociación significativa.

  17. Accuracy of topographic index models at identifying ephemeral gully trajectories on agricultural fields

    Science.gov (United States)

    Sheshukov, Aleksey Y.; Sekaluvu, Lawrence; Hutchinson, Stacy L.

    2018-04-01

    Topographic index (TI) models have been widely used to predict trajectories and initiation points of ephemeral gullies (EGs) in agricultural landscapes. Prediction of EGs strongly relies on the selected value of critical TI threshold, and the accuracy depends on topographic features, agricultural management, and datasets of observed EGs. This study statistically evaluated the predictions by TI models in two paired watersheds in Central Kansas that had different levels of structural disturbances due to implemented conservation practices. Four TI models with sole dependency on topographic factors of slope, contributing area, and planform curvature were used in this study. The observed EGs were obtained by field reconnaissance and through the process of hydrological reconditioning of digital elevation models (DEMs). The Kernel Density Estimation analysis was used to evaluate TI distribution within a 10-m buffer of the observed EG trajectories. The EG occurrence within catchments was analyzed using kappa statistics of the error matrix approach, while the lengths of predicted EGs were compared with the observed dataset using the Nash-Sutcliffe Efficiency (NSE) statistics. The TI frequency analysis produced bi-modal distribution of topographic indexes with the pixels within the EG trajectory having a higher peak. The graphs of kappa and NSE versus critical TI threshold showed similar profile for all four TI models and both watersheds with the maximum value representing the best comparison with the observed data. The Compound Topographic Index (CTI) model presented the overall best accuracy with NSE of 0.55 and kappa of 0.32. The statistics for the disturbed watershed showed higher best critical TI threshold values than for the undisturbed watershed. Structural conservation practices implemented in the disturbed watershed reduced ephemeral channels in headwater catchments, thus producing less variability in catchments with EGs. The variation in critical thresholds for all

  18. [Mild therapeutic hypothermia in cardiogenic shock : Retrospective analysis of 80 patients with preclinical cardiac arrest due to cardiac causes].

    Science.gov (United States)

    Adler, C; Pfister, R; Baldus, S; Reuter, H

    2017-02-01

    The mortality in patients with cardiogenic shock after out-of-hospital cardiac arrest (OHCA) remains high despite advances in resuscitation and early revascularization strategies. The use of mild therapeutic hypothermia (MTH) for improvement of survival and neurological outcome in patients with cardiogenic shock is currently subject to renewed discussion. The aim of this study was the detection of risk factors for mortality and morbidity in patients under MTH in cardiogenic shock following preclinical resuscitation for OHCA. A total of 80 consecutive patients in cardiogenic shock after successful resuscitation (mean age 60 ± 3.2 years) treated with MTH were retrospectively analyzed. Patients were cooled to 33 °C for 24 h using an endovascular cooling device. Neurological outcome was assessed after 2 months based on the Glasgow-Pittsburgh cerebral performance category (CPC) and correlated with various blood parameter values. After 2 months 31 patients (39 %) showed a good neurological recovery with CPC scores of 1-2, 20 patients (25 %) had a poor neurological outcome with CPC scores of 3-4 and 29 (36 %) patients enrolled in the trial died (CPC 5). Patients with a poor outcome showed significantly higher mean serum levels for lactate, creatinine and urea. In addition, these patients showed a continuous increase of serum neuron-specific enolase (NSE) values in contrast to patients with a good outcome (∆ NSE from admission to day 1, CPC 1 and 2: - 10.6 ± 3 µg/l and CPC 3-5: 33 ± 12 µg/l, p = 0.02). Changes in the course of serum creatinine, urea and NSE levels within the first 72 h after OHCA could provide valuable additional information for the early assessment of the neurological prognosis in patients treated with MTH.

  19. Comparison of DNDC and RZWQM2 for simulating hydrology and nitrogen dynamics in a corn-soybean system with a winter cover crop

    Science.gov (United States)

    Desjardins, R.; Smith, W.; Qi, Z.; Grant, B.; VanderZaag, A.

    2017-12-01

    Biophysical models are needed for assessing science-based mitigation options to improve the efficiency and sustainability of agricultural cropping systems. In order to account for trade-offs between environmental indicators such as GHG emissions, soil C change, and water quality it is important that models can encapsulate the complex array of interrelated biogeochemical processes controlling water, nutrient and energy flows in the agroecosystem. The Denitrification Decomposition (DNDC) model is one of the most widely used process-based models, and is arguably the most sophisticated for estimating GHG emissions and soil C&N cycling, however, the model simulates only simple cascade water flow. The purpose of this study was to compare the performance of DNDC to a comprehensive water flow model, the Root Zone Water Quality Model (RZWQM2), to determine which processes in DNDC may be limiting and recommend improvements. Both models were calibrated and validated for simulating crop biomass, soil hydrology, and nitrogen loss to tile drains using detailed observations from a corn-soybean rotation in Iowa, with and without cover crops. Results indicated that crop yields, biomass and the annual estimation of nitrogen and water loss to tiles drains were well simulated by both models (NSE > 0.6 in all cases); however, RZWQM2 performed much better for simulating soil water content, and the dynamics of daily water flow (DNDC: NSE -0.32 to 0.28; RZWQM2: NSE 0.34 to 0.70) to tile drains. DNDC overestimated soil water content near the soil surface and underestimated it deeper in the profile which was presumably caused by the lack of a root distribution algorithm, the inability to simulate a heterogeneous profile and lack of a water table. We recommend these improvements along with the inclusion of enhanced water flow and a mechanistic tile drainage sub-model. The accurate temporal simulation of water and N strongly impacts several biogeochemical processes.

  20. Cerebellar hemangioblastomas: A study of the immunoprofile of neoplastic stromal component

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    Tasić Desanka

    2004-01-01

    Full Text Available Background. Central nervous system hemangioblastomas (HBs are uncommon highly vascularized tumors that are predominantly found in the cerebellum. They occur sporadically or in association with von Hippel-Lindau (VHL disease. HBs are of unknown histogenesis, and the origin of stromal cells is still a subject of debate. The aim of this study was to investigate the immunoprofile of neoplastic stromal component, and to determine whether the profile of the expression of immunomarkers used can contribute to the elucidation of the histogenesis of HBs. Methods. A series of eight cerebellar HBs were histochemically examined for the detection of mast cells and immunohistochemically for the expression of factor VIII-related antigen (FVIII-RAg, CD34, vimentin, factor XIIIa (FXIIIa, S-100 protein, glial fibrillary acidic protein (GFAP, neuron-specific enolase (NSE neurofilaments (NF, synaptophysin, chromogranin, and somatostatin. Results. Mast cells were present in all hemangioblastomas, and were particularly abundant in one tumor. Immunohistochemically, intense reactivity for vimentin and NSE in the stromal cells was constantly seen. Immunoreactivity with S-100 protein and FXIIIa was variable, but generally many HBs stromal cells were negative for these markers. However, stromal cells were uniformly negative for FVIII-RAg in all HBs investigated. They were negative for CD34 GFAP, NF, synaptophysin, chromogranin, as well as somatostatin. GFAP-positivity of the occasional stromal type cells, located only peripherally, was interpreted as "pseudopositivity". Conclusion. The immunoprofile of neoplastic stromal component in this study suggested a possible origin from undifferentiated multipotential mesenchymal cells. High expression of NSE (glycolytic and hypoxia-inducible enzyme in the HBs stromal cells might be related to the loss of the VHL protein function.

  1. Improvement and comparison of likelihood functions for model calibration and parameter uncertainty analysis within a Markov chain Monte Carlo scheme

    Science.gov (United States)

    Cheng, Qin-Bo; Chen, Xi; Xu, Chong-Yu; Reinhardt-Imjela, Christian; Schulte, Achim

    2014-11-01

    In this study, the likelihood functions for uncertainty analysis of hydrological models are compared and improved through the following steps: (1) the equivalent relationship between the Nash-Sutcliffe Efficiency coefficient (NSE) and the likelihood function with Gaussian independent and identically distributed residuals is proved; (2) a new estimation method of the Box-Cox transformation (BC) parameter is developed to improve the effective elimination of the heteroscedasticity of model residuals; and (3) three likelihood functions-NSE, Generalized Error Distribution with BC (BC-GED) and Skew Generalized Error Distribution with BC (BC-SGED)-are applied for SWAT-WB-VSA (Soil and Water Assessment Tool - Water Balance - Variable Source Area) model calibration in the Baocun watershed, Eastern China. Performances of calibrated models are compared using the observed river discharges and groundwater levels. The result shows that the minimum variance constraint can effectively estimate the BC parameter. The form of the likelihood function significantly impacts on the calibrated parameters and the simulated results of high and low flow components. SWAT-WB-VSA with the NSE approach simulates flood well, but baseflow badly owing to the assumption of Gaussian error distribution, where the probability of the large error is low, but the small error around zero approximates equiprobability. By contrast, SWAT-WB-VSA with the BC-GED or BC-SGED approach mimics baseflow well, which is proved in the groundwater level simulation. The assumption of skewness of the error distribution may be unnecessary, because all the results of the BC-SGED approach are nearly the same as those of the BC-GED approach.

  2. Evaluation of electron population terms for 4p, 3p, and (2p): how do HOMO and LUMO shrink or expand depending on nuclear charges?

    Science.gov (United States)

    Nakanishi, Waro; Hayashi, Satoko; Narahara, Kenji; Yamaki, Daisuke; Hada, Masahiko

    2008-01-01

    Electron population terms are evaluated for N=Se, S, and O. Calculations are performed on HOMO and LUMO constructed by pure atomic 4p(Se), 3p(S), and 2p(O) orbitals, employing the 6-311+G(3d) and/or 6-311(++)G(3df,3pd) basis sets at the HF, MP2, and DFT (B3 LYP) levels. Se(4+), Se(2+), Se(0), and Se(2-) with the O(h) symmetry are called G(A: Se) and HSe(+), H(2)Se, and HSe(-) with the C(infinityh) or C(2v) symmetry are named G(B: Se), here [G(A+B: Se) in all]. HOMO and LUMO in G(A+B: N) (N=Se, S, and O) satisfy the conditions of the calculations for . The (4p), (3p), and (2p) values correlate well with the corresponding MO energies (epsilon(N)) for all calculation levels employed. Plots of (HOMO) and (LUMO) versus Q(N) (N=Se, S, and O) at the HF and MP2 levels are analyzed as two correlations. However, the plots at the DFT level can be analyzed as single correlation. A regression curve is assumed for the analysis. Behaviors of clarify how valence orbitals shrink or expand depending on Q(N). The applicability of is examined to establish a new method that enables us to analyze chemical shifts with the charge effect separately from others. A utility program derived from the Gaussian 03 (NMRANAL-NH03G) is applied to evaluate and examine the applicability to the NMR analysis.

  3. Simulating Landscape Sediment Transport Capacity by Using a Modified SWAT Model.

    Science.gov (United States)

    Bonumá, Nadia B; Rossi, Colleen G; Arnold, Jeffrey G; Reichert, José M; Minella, Jean P; Allen, Peter M; Volk, Martin

    2014-01-01

    Sediment delivery from hillslopes to rivers is spatially variable and may lead to long-term delays between initial erosion and related sediment yield at the watershed outlet. Consideration of spatial variability is important for developing sound strategies for water quality improvement and soil protection at the watershed scale. Hence, the Soil and Water Assessment Tool (SWAT) was modified and tested in this study to simulate the landscape transport capacity of sediment. The study area was the steeply sloped Arroio Lino watershed in southern Brazil. Observed sediment yield data at the watershed outlet were used to calibrate and validate a modified SWAT model. For the calibration period, the modified model performed better than the unaltered SWAT2009 version; the models achieved Nash-Sutcliffe efficiency (NSE) values of 0.7 and -0.1, respectively. Nash-Sutcliffe efficiencies were less for the validation period, but the modified model's NSE was higher than the unaltered model (-1.4 and -12.1, respectively). Despite the relatively low NSE values, the results of this first test are promising because the model modifications lowered the percent bias in sediment yield from 73 to 18%. Simulation results for the modified model indicated that approximately 60% of the mobilized soil is deposited along the landscape before it reaches the river channels. This research demonstrates the modified model's ability to simulate sediment yield in watersheds with steep slopes. The results suggest that integration of the sediment deposition routine in SWAT increases accuracy in steeper areas while significantly improving its ability to predict the spatial distribution of sediment deposition areas. Further work is needed regarding (i) improved strategies for spatially distributed sediment transport measurements (for improving process knowledge and model evaluation) and (ii) extensive model tests in other well instrumented experimental watersheds with differing topographic configurations

  4. The biochemical changes in hippocampal formation occurring in normal and seizure experiencing rats as a result of a ketogenic diet.

    Science.gov (United States)

    Chwiej, Joanna; Skoczen, Agnieszka; Janeczko, Krzysztof; Kutorasinska, Justyna; Matusiak, Katarzyna; Figiel, Henryk; Dumas, Paul; Sandt, Christophe; Setkowicz, Zuzanna

    2015-04-07

    In this study, ketogenic diet-induced biochemical changes occurring in normal and epileptic hippocampal formations were compared. Four groups of rats were analyzed, namely seizure experiencing animals and normal rats previously fed with ketogenic (KSE and K groups respectively) or standard laboratory diet (NSE and N groups respectively). Synchrotron radiation based Fourier-transform infrared microspectroscopy was used for the analysis of distributions of the main organic components (proteins, lipids, compounds containing phosphate group(s)) and their structural modifications as well as anomalies in creatine accumulation with micrometer spatial resolution. Infrared spectra recorded in the molecular layers of the dentate gyrus (DG) areas of normal rats on a ketogenic diet (K) presented increased intensity of the 1740 cm(-1) absorption band. This originates from the stretching vibrations of carbonyl groups and probably reflects increased accumulation of ketone bodies occurring in animals on a high fat diet compared to those fed with a standard laboratory diet (N). The comparison of K and N groups showed, moreover, elevated ratios of absorbance at 1634 and 1658 cm(-1) for DG internal layers and increased accumulation of creatine deposits in sector 3 of the Ammon's horn (CA3) hippocampal area of ketogenic diet fed rats. In multiform and internal layers of CA3, seizure experiencing animals on ketogenic diet (KSE) presented a lower ratio of absorbance at 1634 and 1658 cm(-1) compared to rats on standard laboratory diet (NSE). Moreover, in some of the examined cellular layers, the increased intensity of the 2924 cm(-1) lipid band as well as the massifs of 2800-3000 cm(-1) and 1360-1480 cm(-1), was found in KSE compared to NSE animals. The intensity of the 1740 cm(-1) band was diminished in DG molecular layers of KSE rats. The ketogenic diet did not modify the seizure induced anomalies in the unsaturation level of lipids or the number of creatine deposits.

  5. Statistical downscaling of rainfall: a non-stationary and multi-resolution approach

    Science.gov (United States)

    Rashid, Md. Mamunur; Beecham, Simon; Chowdhury, Rezaul Kabir

    2016-05-01

    A novel downscaling technique is proposed in this study whereby the original rainfall and reanalysis variables are first decomposed by wavelet transforms and rainfall is modelled using the semi-parametric additive model formulation of Generalized Additive Model in Location, Scale and Shape (GAMLSS). The flexibility of the GAMLSS model makes it feasible as a framework for non-stationary modelling. Decomposition of a rainfall series into different components is useful to separate the scale-dependent properties of the rainfall as this varies both temporally and spatially. The study was conducted at the Onkaparinga river catchment in South Australia. The model was calibrated over the period 1960 to 1990 and validated over the period 1991 to 2010. The model reproduced the monthly variability and statistics of the observed rainfall well with Nash-Sutcliffe efficiency (NSE) values of 0.66 and 0.65 for the calibration and validation periods, respectively. It also reproduced well the seasonal rainfall over the calibration (NSE = 0.37) and validation (NSE = 0.69) periods for all seasons. The proposed model was better than the tradition modelling approach (application of GAMLSS to the original rainfall series without decomposition) at reproducing the time-frequency properties of the observed rainfall, and yet it still preserved the statistics produced by the traditional modelling approach. When downscaling models were developed with general circulation model (GCM) historical output datasets, the proposed wavelet-based downscaling model outperformed the traditional downscaling model in terms of reproducing monthly rainfall for both the calibration and validation periods.

  6. Integrating 3D geological information with a national physically-based hydrological modelling system

    Science.gov (United States)

    Lewis, Elizabeth; Parkin, Geoff; Kessler, Holger; Whiteman, Mark

    2016-04-01

    Robust numerical models are an essential tool for informing flood and water management and policy around the world. Physically-based hydrological models have traditionally not been used for such applications due to prohibitively large data, time and computational resource requirements. Given recent advances in computing power and data availability, a robust, physically-based hydrological modelling system for Great Britain using the SHETRAN model and national datasets has been created. Such a model has several advantages over less complex systems. Firstly, compared with conceptual models, a national physically-based model is more readily applicable to ungauged catchments, in which hydrological predictions are also required. Secondly, the results of a physically-based system may be more robust under changing conditions such as climate and land cover, as physical processes and relationships are explicitly accounted for. Finally, a fully integrated surface and subsurface model such as SHETRAN offers a wider range of applications compared with simpler schemes, such as assessments of groundwater resources, sediment and nutrient transport and flooding from multiple sources. As such, SHETRAN provides a robust means of simulating numerous terrestrial system processes which will add physical realism when coupled to the JULES land surface model. 306 catchments spanning Great Britain have been modelled using this system. The standard configuration of this system performs satisfactorily (NSE > 0.5) for 72% of catchments and well (NSE > 0.7) for 48%. Many of the remaining 28% of catchments that performed relatively poorly (NSE land cover change studies and integrated assessments of groundwater and surface water resources.

  7. Aspartame and the hippocampus: Revealing a bi-directional, dose/time-dependent behavioural and morphological shift in mice.

    Science.gov (United States)

    Onaolapo, Adejoke Y; Onaolapo, Olakunle J; Nwoha, Polycarp U

    2017-03-01

    Changes, in behaviour, oxidative markers of stress and hippocampal morphology were evaluated following aspartame administration. Mice, (20-22g each) were given vehicle (10ml/kg) or aspartame (20, 40, 80 and 160mg/kg) daily for 28days. They were tested in the Y-maze, radial-arm maze and elevated plus-maze (EPM) after the first and last dose of vehicle or aspartame; and then sacrificed. Hippocampal slices were analysed for aspartic acid, nitric oxide (NO) and superoxide dismutase (SOD); and processed for general histology and neuritic plaques. Glial fibrillary-acid protein (GFAP) expression and neuron-specific enolase (NSE) activities were determined. Radial-arm maze scores increased significantly after acute administration at 80 and 160mg/kg. Repeated administration at 20 and 40mg/kg (Y-maze) and at 40mg/kg (radial-arm maze) was also associated with increased scores, however, performance decreased at higher doses. EPM tests revealed anxiogenic responses following both acute and repeated administration. Significant increase in SOD and NO activities were observed at 40, 80 and 160mg/kg. Neuron counts reduced at higher doses of aspartame. At 40, 80 and 160mg/kg, fewer GFAP-reactive astrocytes were observed in the cornus ammonis, but increased GFAP-reactivity was observed in the dentate gyrus subgranular zone. NSE-positive neurons were readily identifiable within the dentate gyrus at the lower doses of aspartame; but at 160mg/kg, there was marked neuron loss and reduction in NSE-positive neurons. Oral aspartame significantly altered behaviour, anti-oxidant status and morphology of the hippocampus in mice; also, it may probably trigger hippocampal adult neurogenesis. Copyright © 2016 Elsevier Inc. All rights reserved.

  8. [Effect of electroacupuncture on differentiation and proliferation of hippocampal nerve stem cells in splenic asthenia pedo-rats].

    Science.gov (United States)

    Zhuo, Yuan-yuan; Yang, Zhuo-xin; Wu, Jia-man

    2011-10-01

    To observe the effect of electroacupuncture (EA) on the differentiation and proliferation of nerve stem cells in the hippocampal dentate gyrus (DG) in splenic asthenia pedo-rats so as to study its central mechanism. A total of 72 SD male rats were randomly assigned to normal control group (n=24), model group (n=24) and EA group (n=24) which were further divided into 7 d, 14 d, 28 d and 49 d time-points (n=6). Splenic asthenia model was established by intraperitoneal injection of reserpine and gavage of Dahuang (Radix et Rhizoma Rhei) fluid. EA was applied to bilateral "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) for 20 min, once daily for 7, 14, 28 and 49 days respectively. Brdu, Nestin, glial fibrillary acidic protein (GFAP), and neuron-specific enolase (NSE) expression in the DG of hippocampus were detected by immunohistochemistry double staining. Compared with the normal control group, the numbers of Brdu, Brdu/GFAP, Brdu/NSE Immunoreactive (IR) positive cells in the DG of hippocampus on day 7 and 14, and that of Brdu/Nestin IR-positive cells on day 7 were decreased considerably in the model group (P 0.05). EA of ST 36 and SP 6 can effectively suppress splenic asthenia syndrome-induced decrease of the numbers of Brdu, Brdu/GFAP, Brdu/Nestin and Brdu/NSE IR-positive cells in the DG of hippocampus at the early stage in the splenic asthenia rats, which may contribute to its effect in improving splenic asthenia symptoms in clinic by promoting the proliferation and differentiation of some nerve stem cells in the hippocampus.

  9. Costs and cost-driving factors for acute treatment of adults with status epilepticus: A multicenter cohort study from Germany.

    Science.gov (United States)

    Kortland, Lena-Marie; Alfter, Anne; Bähr, Oliver; Carl, Barbara; Dodel, Richard; Freiman, Thomas M; Hubert, Kristina; Jahnke, Kolja; Knake, Susanne; von Podewils, Felix; Reese, Jens-Peter; Runge, Uwe; Senft, Christian; Steinmetz, Helmuth; Rosenow, Felix; Strzelczyk, Adam

    2016-12-01

    To provide first data on inpatient costs and cost-driving factors due to nonrefractory status epilepticus (NSE), refractory status epilepticus (RSE), and super-refractory status epilepticus (SRSE). In 2013 and 2014, all adult patients treated due to status epilepticus (SE) at the university hospitals in Frankfurt, Greifswald, and Marburg were analyzed for healthcare utilization. We evaluated 341 admissions in 316 patients (65.7 ± [standard deviation]18.2 years; 135 male) treated for SE. Mean costs of hospital treatment were €14,946 (median €5,278, range €776-€152,911, €787 per treatment day) per patient per admission, with a mean length of stay (LOS) of 19.0 days (median 14.0, range 1-118). Course of SE had a significant impact on mean costs, with €8,314 in NSE (n = 137, median €4,597, €687 per treatment day, 22.3% of total inpatient costs due to SE), €13,399 in RSE (n = 171, median €7,203, €638/day, 45.0% of total costs, p 14 days. Overall mortality at discharge was 14.4% and significantly higher in RSE/SRSE (20.1%) than in NSE (5.8%). Acute treatment of SE, and particularly SRSE and ventilation, are associated with high hospital costs and prolonged LOS. Extrapolation to the whole of Germany indicates that SE causes hospital costs of >€200 million per year. Along with the demographic change, incidence of SE will increase and costs for hospital treatment and sequelae of SE will rise. Wiley Periodicals, Inc. © 2016 International League Against Epilepsy.

  10. STUDY OF THE DETONATION PHASE IN THE GRAVITATIONALLY CONFINED DETONATION MODEL OF TYPE Ia SUPERNOVAE

    International Nuclear Information System (INIS)

    Meakin, Casey A.; Townsley, Dean; Jordan, George C.; Truran, James; Lamb, Don; Seitenzahl, Ivo

    2009-01-01

    We study the gravitationally confined detonation (GCD) model of Type Ia supernovae (SNe Ia) through the detonation phase and into homologous expansion. In the GCD model, a detonation is triggered by the surface flow due to single-point, off-center flame ignition in carbon-oxygen white dwarfs (WDs). The simulations are unique in terms of the degree to which nonidealized physics is used to treat the reactive flow, including weak reaction rates and a time-dependent treatment of material in nuclear statistical equilibrium (NSE). Careful attention is paid to accurately calculating the final composition of material which is burned to NSE and frozen out in the rapid expansion following the passage of a detonation wave over the high-density core of the WD; and an efficient method for nucleosynthesis postprocessing is developed which obviates the need for costly network calculations along tracer particle thermodynamic trajectories. Observational diagnostics are presented for the explosion models, including abundance stratifications and integrated yields. We find that for all of the ignition conditions studied here a self-regulating process comprised of neutronization and stellar expansion results in final 56 Ni masses of ∼1.1 M sun . But, more energetic models result in larger total NSE and stable Fe-peak yields. The total yield of intermediate mass elements is ∼0.1 M sun and the explosion energies are all around 1.5 x 10 51 erg. The explosion models are briefly compared to the inferred properties of recent SN Ia observations. The potential for surface detonation models to produce lower-luminosity (lower 56 Ni mass) SNe is discussed.

  11. Variables que influyen en la satisfacción con la vida de personas de distinto nivel socioeconómico en el sur de Chile

    Directory of Open Access Journals (Sweden)

    Berta Schnettler

    2014-06-01

    Full Text Available El presente estudio tiene por objeto identificar variables que influyen en la satisfacción con la vida de personas pertenecientes a distintos niveles socioeconómicos (NSE en el sur de Chile. Para lograr este objetivo, 316 residentes en las principales ciudades de esta parte del país, distribuidos proporcionalmente por comuna, respondieron un cuestionario que incluía las escalas SWLS (Satisfacción con la vida y SWFL (Satisfacción con la alimentación, el Índice de Calidad de Vida Relativo a la Salud (ICVRS y la Escala de Discrepancia del Yo. Además se consultaron las características demográficas de los encuestados. Como resultado se obtuvo que la mayor proporción de personas extremadamente satisfechas con su vida y su alimentación se encuentran en el NSE más alto. Asimismo, se plantearon cuatro modelos logit ordinales, en los cuales la variable dependiente fue la satisfacción con la vida, considerando la muestra total y tres submuestras: estratos alto y medio-alto, medio-medio y en conjunto los niveles medio-bajo, bajo y muy bajo. Estos cuatro modelos resultaron significativos, pero difirieron en las variables explicativas que fueron significativas. También se evidenció que, independientemente del NSE, las personas con alta satisfacción con su alimentación tienen mayor probabilidad de tener alta satisfacción con la vida. Por último, se obtuvo que otras variables que se relacionan con la satisfacción con la vida son la salud, la familia, la discrepancia con el yo ideal, la edad y la tenencia de bienes en el hogar.

  12. Use of Tethered Enzymes as a Platform Technology for Rapid Analyte Detection

    Science.gov (United States)

    Cohen, Roy; Lata, James P.; Lee, Yurim; Hernández, Jean C. Cruz; Nishimura, Nozomi; Schaffer, Chris B.; Mukai, Chinatsu; Nelson, Jacquelyn L.; Brangman, Sharon A.; Agrawal, Yash; Travis, Alexander J.

    2015-01-01

    Background Rapid diagnosis for time-sensitive illnesses such as stroke, cardiac arrest, and septic shock is essential for successful treatment. Much attention has therefore focused on new strategies for rapid and objective diagnosis, such as Point-of-Care Tests (PoCT) for blood biomarkers. Here we use a biomimicry-based approach to demonstrate a new diagnostic platform, based on enzymes tethered to nanoparticles (NPs). As proof of principle, we use oriented immobilization of pyruvate kinase (PK) and luciferase (Luc) on silica NPs to achieve rapid and sensitive detection of neuron-specific enolase (NSE), a clinically relevant biomarker for multiple diseases ranging from acute brain injuries to lung cancer. We hypothesize that an approach capitalizing on the speed and catalytic nature of enzymatic reactions would enable fast and sensitive biomarker detection, suitable for PoCT devices. Methods and findings We performed in-vitro, animal model, and human subject studies. First, the efficiency of coupled enzyme activities when tethered to NPs versus when in solution was tested, demonstrating a highly sensitive and rapid detection of physiological and pathological concentrations of NSE. Next, in rat stroke models the enzyme-based assay was able in minutes to show a statistically significant increase in NSE levels in samples taken 1 hour before and 0, 1, 3 and 6 hours after occlusion of the distal middle cerebral artery. Finally, using the tethered enzyme assay for detection of NSE in samples from 20 geriatric human patients, we show that our data match well (r = 0.815) with the current gold standard for biomarker detection, ELISA—with a major difference being that we achieve detection in 10 minutes as opposed to the several hours required for traditional ELISA. Conclusions Oriented enzyme immobilization conferred more efficient coupled activity, and thus higher assay sensitivity, than non-tethered enzymes. Together, our findings provide proof of concept for using

  13. Use of Tethered Enzymes as a Platform Technology for Rapid Analyte Detection.

    Directory of Open Access Journals (Sweden)

    Roy Cohen

    Full Text Available Rapid diagnosis for time-sensitive illnesses such as stroke, cardiac arrest, and septic shock is essential for successful treatment. Much attention has therefore focused on new strategies for rapid and objective diagnosis, such as Point-of-Care Tests (PoCT for blood biomarkers. Here we use a biomimicry-based approach to demonstrate a new diagnostic platform, based on enzymes tethered to nanoparticles (NPs. As proof of principle, we use oriented immobilization of pyruvate kinase (PK and luciferase (Luc on silica NPs to achieve rapid and sensitive detection of neuron-specific enolase (NSE, a clinically relevant biomarker for multiple diseases ranging from acute brain injuries to lung cancer. We hypothesize that an approach capitalizing on the speed and catalytic nature of enzymatic reactions would enable fast and sensitive biomarker detection, suitable for PoCT devices.We performed in-vitro, animal model, and human subject studies. First, the efficiency of coupled enzyme activities when tethered to NPs versus when in solution was tested, demonstrating a highly sensitive and rapid detection of physiological and pathological concentrations of NSE. Next, in rat stroke models the enzyme-based assay was able in minutes to show a statistically significant increase in NSE levels in samples taken 1 hour before and 0, 1, 3 and 6 hours after occlusion of the distal middle cerebral artery. Finally, using the tethered enzyme assay for detection of NSE in samples from 20 geriatric human patients, we show that our data match well (r = 0.815 with the current gold standard for biomarker detection, ELISA-with a major difference being that we achieve detection in 10 minutes as opposed to the several hours required for traditional ELISA.Oriented enzyme immobilization conferred more efficient coupled activity, and thus higher assay sensitivity, than non-tethered enzymes. Together, our findings provide proof of concept for using oriented immobilization of active

  14. Trifluoromethylthiolation and Trifluoromethylselenolation of α-Diazo Esters Catalyzed by Copper.

    Science.gov (United States)

    Matheis, Christian; Krause, Thilo; Bragoni, Valentina; Goossen, Lukas J

    2016-08-22

    α-Diazo esters are smoothly converted into the corresponding trifluoromethyl thio- or selenoethers by reaction with Me4 NSCF3 or Me4 NSeCF3 , respectively, in the presence of catalytic amounts of copper thiocyanate. This straightforward method gives high yields under neutral conditions at room temperature and is applicable to a wide range of functionalized molecules, including diverse α-amino acid derivatives. It is well-suited for the late-stage introduction of trifluoromethylthio or -seleno groups into drug-like molecules. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Svært ved grammatikken - del 2

    DEFF Research Database (Denmark)

    Kristensen, Line Burholt; Boye, Kasper

    2016-01-01

    Denne artikel er anden del af en artikelserie om baggrunden for grammatiske vanskeligheder. Den første artikel (bragt i Logos nr. 75) forklarede hvorfor grammatik er vigtigt for mennesker, og hvorfor det er vigtigt at have en teori om grammatik når man skal afgrænse, undersøge og behandle...... grammatiske vanskeligheder. Artiklen viste også at en klar og praktisk anvendelig definition er nødvendig, men at eksisterende traditionelle teorier giver uklare kriterier for forskellen på grammatik og leksikon. I denne anden del af artikelserien præsenterer vi en ny teori om grammatisk status (Boye & Harder...

  16. Neutron scattering. Experiment manuals

    Energy Technology Data Exchange (ETDEWEB)

    Brueckel, Thomas; Heger, Gernot; Richter, Dieter; Roth, Georg; Zorn, Reiner (eds.)

    2010-07-01

    The following topics are dealt with: The thermal triple axis spectrometer PUMA, the high-resolution powder diffractometer SPODI, the hot single-crystal diffractometer HEiDi for structure analysis with neutrons, the backscattering spectrometer SPHERES, neutron polarization analysis with tht time-of-flight spectrometer DNS, the neutron spin-echo spectrometer J-NSE, small-angle neutron scattering with the KWS-1 and KWS-2 diffractometers, the very-small-angle neutron scattering diffractrometer with focusing mirror KWS-3, the resonance spin-echo spectrometer RESEDA, the reflectometer TREFF, the time-of-flight spectrometer TOFTOF. (HSI)

  17. Crossover from Rouse dynamics to the α- relaxation in a poly(vinyl ethylene)

    International Nuclear Information System (INIS)

    Arbe, A.; Colmenero, J.; Richter, D.; Monkenbusch, M.; Willner, L.; Farago, B.

    2004-01-01

    By means of neutron spin echo (NSE) we have explored the dynamics of poly(vinyl ethylene) on length scales covering Rouse dynamics and below. The results establish the simultaneous existence of a generic sublinear diffusion regime which underlies the α-process in addition to the Rouse process. Both regimes are separated by a well- defined dynamic crossover. From that the size of the Gaussian blobs making up the Rouse model is determined directly. The glassy dynamics may thus be identified with subdiffusive motions occurring within these Gaussian blobs. (author)

  18. El desarrollo del pensamiento lógico verbal y abstracto en educación inicial

    OpenAIRE

    Pérez Narváez, Marco Vinicio

    2016-01-01

    El presente trabajo académico consiste en presentar un marco teórico sobre el desarrollo del pensamiento lógico verbal y abstracto en educación inicial, a través del análisis de la propuesta curricular emitida por el Ministerio de Educación del Ecuador.A partir de estainvestigaciónse plantean estrategias para desarrollar el pensamiento lógico verbal y abstracto en el nivel educativo inicial uno y dos. Se analiza el concepto de inteligencia y desarrollo del pensamiento; así como diversos enfoq...

  19. Estrés Materno y Configuración Familiar: Estudio comparativo en Familias Chilenas Monoparentales y Nucleares de bajos ingresos

    OpenAIRE

    Olhaberry, Marcia; Pontificia Universidad Católica de Chile; Farkas, Chamarrita

    2012-01-01

    Los estudios sobre estrés materno durante la crianza infantil han considerado variables contextuales para explicarlo. El nivel socioeconómico, así como la configuración familiar han sido variables relevantes, asociándose monoparentalidad en familias de bajos ingresos a mayores niveles de estrés materno. Se estudian los niveles de estrés materno en familias chilenas nucleares y monoparentales de N.S.E. bajo, considerando el estrés en distintas dimensiones, asociado al rol materno, a la interac...

  20. Emerging Role of the Army Family Physician in Primary Health Care Delivery.

    Science.gov (United States)

    1976-12-13

    ev.~ ~ ~ i Defe nse or any of its egeflC .eS. l~ iIs ~ ‘j~ Uii~ fi ~ ~~~~ ~ t e tSh5 ~S~ t t~ )i O~. efl pub hiCati0 fl unt il it has been c)eafe...t, dedicated capable staff , talented residents, adequate resources , teaching depth, credibility with the resi- dents and the rest of the medical...CLASSIFICATION OF THIS PAOI(W&~~ D~~a I&e.,.4) —.---. ~~~ support , dedicated capable staff, talented’res~dents, adequate resources, ‘ teaching depth

  1. Cerebral formation of free radicals during hypoxia does not cause structural damage and is associated with a reduction in mitochondrial PO2; evidence of O2-sensing in humans?

    DEFF Research Database (Denmark)

    Bailey, Damian M; Taudorf, Sarah; Berg, Ronan M G

    2011-01-01

    Cellular hypoxia triggers a homeostatic increase in mitochondrial free radical signaling. In this study, blood was obtained from the radial artery and jugular venous bulb in 10 men during normoxia and 9¿ hours hypoxia (12.9% O(2)). Mitochondrial oxygen tension (p(O(2))(mit)) was derived from...... cerebral blood flow and blood gases. The ascorbate radical (A(•-)) was detected by electron paramagnetic resonance spectroscopy and neuron-specific enolase (NSE), a biomarker of neuronal injury, by enzyme-linked immunosorbent assay. Hypoxia increased the cerebral output of A(•-) in proportion...

  2. Cerebral formation of free radicals during hypoxia does not cause structural damage and is associated with a reduction in mitochondrial PO2; evidence of O2-sensing in humans?

    DEFF Research Database (Denmark)

    Bailey, Damian M; Taudorf, Sarah; Berg, Ronan M G

    2011-01-01

    Cellular hypoxia triggers a homeostatic increase in mitochondrial free radical signaling. In this study, blood was obtained from the radial artery and jugular venous bulb in 10 men during normoxia and 9  hours hypoxia (12.9% O(2)). Mitochondrial oxygen tension (p(O(2))(mit)) was derived from...... cerebral blood flow and blood gases. The ascorbate radical (A(•-)) was detected by electron paramagnetic resonance spectroscopy and neuron-specific enolase (NSE), a biomarker of neuronal injury, by enzyme-linked immunosorbent assay. Hypoxia increased the cerebral output of A(•-) in proportion...

  3. Neutron scattering. Experiment manuals

    International Nuclear Information System (INIS)

    Brueckel, Thomas; Heger, Gernot; Richter, Dieter; Roth, Georg; Zorn, Reiner

    2012-01-01

    The following topics are dealt with: The thermal triple-axis spectrometer PUMA, the high-resolution powder diffractometer SPODI, the hot single-crystal diffractometer HEiDi for structure analysis with neutrons, the backscattering spectrometer SPHERES, the neutron polarization analyzer DNS, the neutron spin-echo spectrometer J-NSE, the small-angle neutron diffractometers KWS-1/-2, the very-small-angle neutron diffractometer with focusing mirror KWS-3, the resonance spin-echo spectrometer RESEDA, the reflectometer TREFF, the time-of-flight spectrometer TOFTOF. (HSI)

  4. Rat Plasma Oxidation Status After Nigella Sativa L. Botanical Treatment in CCL(4)-Treated Rats.

    Science.gov (United States)

    Soleimani, Hengameh; Ranjbar, Akram; Baeeri, Maryam; Mohammadirad, Azadeh; Khorasani, Reza; Yasa, Narguess; Abdollahi, Mohammad

    2008-01-01

    ABSTRACT Nigella sativa Linn. (family Ranunculaceae), commonly known as black cumin, is native to the Mediterranean area and has been used for thousands of years as a health and beauty aid. The present study investigated the protective effects of Nigella sativa (NS) extract (NSE) and oil (NSO) on CCl(4)-induced nitrosative stress and protein oxidation in rat. CCl(4) (0.8 mg/kg) was used as an aid for induction of nitrosative stress. In vitro antioxidant potential was tested in the presence of 2,4-dinitrophenylhyrdazine (DPPH) as an organic nitrogen radical. Doses of 0.2, 0.3, and 1 mg/kg of the NS extract and oil were administered to CCL(4)-treated rats for 10 days. At the end of treatment, blood was taken from rats under anesthesia and plasma was separated. The concentration of nitric oxide (NO), total antioxidant power (TAP), carbonyl molecules (CM) as measure of protein oxidation (PO), tumor necrosis factor-alpha (TNF-alpha), and total thiol molecules (TTM) were measured in plasma. In vitro evaluation of antioxidant effects of NSE and NSO showed that the highest antioxidant activity (80%) was observed with the concentration of 10 and 20 mg/ml, respectively, that were equal to vitamin E (200 mg/ml). Administration of CCL(4) increased plasma PO, NO, TNF-alpha and decreased TAP and TTM. Both NSE and NSO showed significant protection against CCl(4)-induced changes in biochemical parameters, but not dose-dependently. Doses of 0.3 and 1 mg/kg were more effective than doses of 0.2 mg/kg for both NSE and NSO, but dose of 1 mg/kg was the most effective one. The results indicate the potential of NS in preventing CCL(4)-induced toxic nitrosative stress. It is concluded that NS has marked antioxidant potentials that may be beneficial in alleviating complications of many illnesses related to oxidative/nitrosative stress in humans, but preclinical safety measures should be completed before clinical trials.

  5. Demens Film

    DEFF Research Database (Denmark)

    Jensen, Anders Møller

    2012-01-01

    Vi vil skabe film til mennesker med demens – ikke film om demens sygdommen eller beretninger om livet og hverdagen med en kronisk lidelse. Filmene skal medvirke til at frembringe en behagelig stemning omkring og hos mennesker med demens, så hverdagen bliver så tryg som mulig. Filmene skal samtidig...... var at afgrænse og prioritere projektet, samt komme med anbefalinger omkring hvad der er vigtigt, i forbindelse med produktion af film målrettet mennesker med demens. Resultat af ekspertgruppen sammenfattes i denne rapport. Projektet gennemføres som et samarbejde mellem Retrospect Film...

  6. Observation of undulation motion of lipid bilayers by neutron spin echo

    International Nuclear Information System (INIS)

    Yamada, Norifumi L.; Seto, Hideki; Hishida, Mafumi

    2010-01-01

    Aqueous solutions of synthesized phospholipids have been well investigated as model biomembranes. These lipids usually self-assemble into regular stacks of bilayers with a characteristic repeat distance on the order of nm, whereas real biomembrane exist as single bilayers. The key phenomenon in understanding the formation of single isolated bilayers in 'unbinding' of lipid bilayers, in which the inter-bilayer distance of lipid bilayers diverges by the steric interaction due to the membrane undulation. In this paper, we show some results of neutron spin-echo (NSE) experiments to investigate the effect of the steric interaction on unbinding and related phenomena. (author)

  7. Serial Sampling of Serum Protein Biomarkers for Monitoring Human Traumatic Brain Injury Dynamics: A Systematic Review.

    Science.gov (United States)

    Thelin, Eric Peter; Zeiler, Frederick Adam; Ercole, Ari; Mondello, Stefania; Büki, András; Bellander, Bo-Michael; Helmy, Adel; Menon, David K; Nelson, David W

    2017-01-01

    The proteins S100B, neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), and neurofilament light (NF-L) have been serially sampled in serum of patients suffering from traumatic brain injury (TBI) in order to assess injury severity and tissue fate. We review the current literature of serum level dynamics of these proteins following TBI and used the term "effective half-life" ( t 1/2 ) in order to describe the "fall" rate in serum. Through searches on EMBASE, Medline, and Scopus, we looked for articles where these proteins had been serially sampled in serum in human TBI. We excluded animal studies, studies with only one presented sample and studies without neuroradiological examinations. Following screening (10,389 papers), n  = 122 papers were included. The proteins S100B ( n  = 66) and NSE ( n  = 27) were the two most frequent biomarkers that were serially sampled. For S100B in severe TBI, a majority of studies indicate a t 1/2 of about 24 h, even if very early sampling in these patients reveals rapid decreases (1-2 h) though possibly of non-cerebral origin. In contrast, the t 1/2 for NSE is comparably longer, ranging from 48 to 72 h in severe TBI cases. The protein GFAP ( n  = 18) appears to have t 1/2 of about 24-48 h in severe TBI. The protein UCH-L1 ( n  = 9) presents a t 1/2 around 7 h in mild TBI and about 10 h in severe. Frequent sampling of these proteins revealed different trajectories with persisting high serum levels, or secondary peaks, in patients with unfavorable outcome or in patients developing secondary detrimental events. Finally, NF-L ( n  = 2) only increased in the few studies available, suggesting a serum availability of >10 days. To date, automated assays are available for S100B and NSE making them faster and more practical to use. Serial sampling of brain-specific proteins in serum reveals different temporal trajectories that should be

  8. Pleomorphic (giant cell) carcinoma of the intestine. An immunohistochemical and electron microscopic study

    DEFF Research Database (Denmark)

    Bak, Martin; Teglbjaerg, P S

    1989-01-01

    reaction for neuron-specific enolase (NSE) was found in three tumors and a positive reaction for chromogranin was found in one tumor. On electron microscopic study, intracytoplasmic whorls of intermediate filaments were seen in the perinuclear area. Dense core "neurosecretory" granules were rarely seen......Pleomorphic (giant cell) carcinomas have been described in the lungs, thyroid, pancreas, and gallbladder. Two pleomorphic carcinomas of the small bowel and two of the large bowel are presented. On light microscopic study, the carcinomas were solid, without squamous or glandular differentiation...

  9. Development of a Regularized Dynamic System Response Curve for Real-Time Flood Forecasting Correction

    Directory of Open Access Journals (Sweden)

    Yiqun Sun

    2018-04-01

    Full Text Available The dynamic system response curve (DSRC is commonly applied as a real-time flood forecasting error correction method to improve the accuracy of real-time flood forecasting. It has been widely recognized that the least squares (OLS/LS method, employed by DSRC, breaks down ill-posed problems, and therefore, the DSRC method may lead to deterioration in performance caused by meaningless solutions. To address this problem, a diagnostically theoretical analysis was conducted to investigate the relationship between the numerical solution of the Fredholm equation of the first kind and the DSRC method. The analysis clearly demonstrates the derivation of the problem and has implications for an improved approach. To overcome the unstable problem, a new method using regularization techniques (Tikhonov regularization and L-Curve criterion is proposed. Moreover, in this study, to improve the performance of hydrological models, the new method is used as an error correction method to correct a variable from a hydrological model. The proposed method incorporates the information from a hydrological model structure. Based on the analysis of the hydrological model, the free water storage of the Xinanjiang rainfall-runoff (XAJ model is corrected to improve the model’s performance. A numerical example and a real case study are presented to compare the two methods. Results from the numerical example indicate that the mean Nash–Sutcliffe efficiency value (NSE of the regularized DSRC method (RDSRC decreased from 0.99 to 0.55, while the mean NSE of DSRC decreased from 0.98 to −1.84 when the noise level was increased. The overall performance measured by four different criteria clearly demonstrates the robustness of the RDSRC method. Similar results were obtained for the real case study. The mean NSE of 35 flood events obtained by RDSRC method was 0.92, which is significantly higher than the mean NSE of DSRC (0.7. The results demonstrate that the RDSRC method is much

  10. Neutron scattering. Experiment manuals

    International Nuclear Information System (INIS)

    Brueckel, Thomas; Heger, Gernot; Richter, Dieter; Roth, Georg; Zorn, Reiner

    2014-01-01

    The following topics are dealt with: The thermal triple-axis spectrometer PUMA, the high-resolution powder diffractometer SPODI, the hot-single-crystal diffractometer HEiDi, the three-axis spectrometer PANDA, the backscattering spectrometer SPHERES, the DNS neutron-polarization analysis, the neutron spin-echo spectrometer J-NSE, small-angle neutron scattering at KWS-1 and KWS-2, a very-small-angle neutron scattering diffractometer with focusing mirror, the reflectometer TREFF, the time-of-flight spectrometer TOFTOF. (HSI)

  11. Serial Sampling of Serum Protein Biomarkers for Monitoring Human Traumatic Brain Injury Dynamics: A Systematic Review

    Directory of Open Access Journals (Sweden)

    Eric Peter Thelin

    2017-07-01

    Full Text Available BackgroundThe proteins S100B, neuron-specific enolase (NSE, glial fibrillary acidic protein (GFAP, ubiquitin carboxy-terminal hydrolase L1 (UCH-L1, and neurofilament light (NF-L have been serially sampled in serum of patients suffering from traumatic brain injury (TBI in order to assess injury severity and tissue fate. We review the current literature of serum level dynamics of these proteins following TBI and used the term “effective half-life” (t1/2 in order to describe the “fall” rate in serum.Materials and methodsThrough searches on EMBASE, Medline, and Scopus, we looked for articles where these proteins had been serially sampled in serum in human TBI. We excluded animal studies, studies with only one presented sample and studies without neuroradiological examinations.ResultsFollowing screening (10,389 papers, n = 122 papers were included. The proteins S100B (n = 66 and NSE (n = 27 were the two most frequent biomarkers that were serially sampled. For S100B in severe TBI, a majority of studies indicate a t1/2 of about 24 h, even if very early sampling in these patients reveals rapid decreases (1–2 h though possibly of non-cerebral origin. In contrast, the t1/2 for NSE is comparably longer, ranging from 48 to 72 h in severe TBI cases. The protein GFAP (n = 18 appears to have t1/2 of about 24–48 h in severe TBI. The protein UCH-L1 (n = 9 presents a t1/2 around 7 h in mild TBI and about 10 h in severe. Frequent sampling of these proteins revealed different trajectories with persisting high serum levels, or secondary peaks, in patients with unfavorable outcome or in patients developing secondary detrimental events. Finally, NF-L (n = 2 only increased in the few studies available, suggesting a serum availability of >10 days. To date, automated assays are available for S100B and NSE making them faster and more practical to use.ConclusionSerial sampling of brain-specific proteins in serum reveals

  12. Fullerene-containing polymeric stars in bulk and solution by neutron spin-echo

    CERN Document Server

    Lebedev, V T; Toeroek, G; Cser, L; Bershtein, V A; Zgonnik, V N; Melenevskaya, E Y; Vinogradova, L V

    2002-01-01

    Stars with C sub 6 sub 0 fullerene core and poly (styrene) (PS) arms have been studied in benzene and in the bulk by neutron spin echo (NSE). Behaviours of stars (six arms, each with a mass M=5.10 sup 3) at momentum transfer q=0.2-0.6 nm sup - sup 1 in the time range t=0.01-20 ns at temperatures T=20-60 C were compared with dynamics of free PS chains. Displaying depressed molecular mobility, the stars did not obey the usual dynamic Zimm or Rouse model. The fullerene polymer interaction at a specific molecular architecture results in oscillating dynamics. (orig.)

  13. GEL DE ALOE-VERA COMO POTENCIAL INHIBIDOR DE LA CORROSIÓN DEL ACERO DE REFUERZO ESTRUCTURAL

    OpenAIRE

    Héctor Herrera-Hernández

    2015-01-01

    En el presente trabajo se estudiaelectroquímicamente un GELextracto de las hojas de ALOE VERAcomo un posible inhibidor de la corrosión del acero de refuerzo estructuraldel concreto.Los estudiosde corrosiónse llevaron a cabo en 1M de HCl y utilizando la técnica de espectroscopia de impedancia electroquímica (EIS)se evaluó la resistencia a la corrosión en presencia y ausencia del GEL.Los diagramas de impedancia mostraronque la adición en diferentes proporciones...

  14. Isolation of furocoumarins from bergamot fruits as HL-60 differentiation-inducing compounds.

    Science.gov (United States)

    Kawaii, S; Tomono, Y; Katase, E; Ogawa, K; Yano, M

    1999-10-01

    The HL-60 differentiation-inducing compounds in bergamot fruits were isolated with column chromatography and identified as bergamottin, bergapten, and citropten by (1)H and (13)C NMR. Their HL-60 differentiation-inducing activity was measured by examining nitro blue tetrazolium (NBT) reducing, nonspecific acid esterase (NSE), specific esterase (SE), and phagocytic activities, and bergamottin showed the strongest activity among the coumarins isolated from bergamot fruits. The structure-activity relationship obtained from HL-60 differentiation assay suggests that hydrophobicity of furocoumarins is correlated with their activity.

  15. Nutritional status of urban schoolchildren of high and low socioeconomic status in Quetzaltenango, Guatemala Estado nutricional de escolares urbanos de niveles socioeconómicos alto y bajo en Quetzaltenango, Guatemala

    Directory of Open Access Journals (Sweden)

    Iris F. Groeneveld

    2007-09-01

    Full Text Available OBJECTIVE: The prevalence of overweight and obesity is growing in children in many developing countries, increasing chronic disease risk. Our objective was to assess the prevalence of stunting, underweight, overweight, and obesity in schoolchildren 8 to 10 years old who were of high or low socioeconomic status (SES in Quetzaltenango, which is the second largest city in Guatemala METHODS: Between April and June 2005 we conducted a cross-sectional survey among 583 children in private and public elementary schools, in which we measured height and weight. The Centers for Disease Control and Prevention (CDC 2000 height-for-age z-scores, weight-for-age z-scores, and body mass index-for-age centiles were used to define stunting, underweight, overweight, and obesity. RESULTS: Mean height, weight, and body mass index were significantly higher in the 327 children of high SES than in the 256 children of low SES, across sexes and age groups. The prevalence of stunting was significantly higher in low-SES children than in high-SES ones (27.0% vs. 7.3%, P OBJETIVO: En muchos países en desarrollo se elevan las prevalencias de sobrepeso y de obesidad en niños, con el incremento del riesgo de enfermedades crónicas. El objetivo de este trabajo fue evaluar las prevalencias de retraso en el crecimiento, peso bajo, sobrepeso y obesidad en escolares de 8 a 10 años de edad de niveles socioeconómicos (NSE alto o bajo en Quetzaltenango, la segunda mayor ciudad de Guatemala. MÉTODOS: Se realizó un estudio transversal entre abril y junio de 2005 en el que se midió el peso y la talla de 583 niños de escuelas primarias privadas y públicas. Para evaluar el retraso en el crecimiento, el peso bajo, el sobrepeso y la obesidad se utilizaron como referencias las puntuaciones z de la talla para la edad y del peso para la edad y los centiles de los índices de masa corporal para la edad, propuestos por los Centros para el Control y la Prevención de las Enfermedades (CDC

  16. Marcadores Hemoquímicos de afectación al tejido nervioso como indicadores de daño en la hipertensión arterial esencial y en el ictus

    OpenAIRE

    González García, Sergio

    2014-01-01

    En el presente trabajo se aborda la aplicabilidad clínica de las determinaciones séricas de algunos marcadores hemoquímicos de daño del SNC (NSE y S100B), inflamatorios (PCR, C3, C4 y AAT), de trombosis (fibrinógeno y DD) y de disfunción endotelial (μalb) en dos enfermedades íntimamente relacionadas, donde la afectación vascular constituye la base fisiopatológica: la HTA esencial y el ictus agudo. El objetivo principal de esta investigación fue evaluar la utilidad de marcadores hemoquímicos c...

  17. Advanced Rechargeable Lithium Sulfur Dioxide Cell

    Science.gov (United States)

    1991-11-01

    AD-A274 908IIIIlIIIE McDonald , P. Harris, F. Goebel, S. Hossi ierra, M. Guentert, C. Todino 7 ad r nse TECHNICAL PRODUCTS INCY DTIC ELECTE JAN26 1994...Pawcatuck, CT 06379 94-02298 1425 Best Available Copy I ADVANCED RECHARGEABLE LITHIUM SULFUR DIOXIDE CELL I R.C. McDonald , P. Harris, F. Goebel, S. Hossain...20 minutes. The electrochemical measurements were carried out using a I Starbuck 20-station cycler system which is connected to a computer to monitor

  18. Update History of This Database - Trypanosomes Database | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Trypanosomes Database Update History of This Database Date Update contents 2014/05/07 The co...ntact information is corrected. The features and manner of utilization of the database are corrected. 2014/02/04 Trypanosomes Databas...e English archive site is opened. 2011/04/04 Trypanosomes Database ( http://www.tan...paku.org/tdb/ ) is opened. About This Database Database Description Download Lice...nse Update History of This Database Site Policy | Contact Us Update History of This Database - Trypanosomes Database | LSDB Archive ...

  19. Limitaciones del IMC como indicador exclusivo de estado nutricional

    OpenAIRE

    Moreno Romero, Susana; Dipierri, José Edgardo; Marrodán, María D.

    2007-01-01

    El presente trabajo tiene como objetivo determinar el potencial diagnóstico y las limitaciones del uso del Índice de Masa Corporal como único indicador del estado nutricional. Para ello se analizó una muestra constituida por 2155 sujetos de ambos sexos, con edades comprendidas entre los 6 y 18 años y pertenecientes a tres poblaciones jujeñas que difieren en ubicación altitudinal y nivel socieconómico (NSE): 1.) Susques, localidad rural situada a 3675 m.s.n.m; 2.) Alto Comedero, barrio perifér...

  20. The Time-Varying Risk and Return Trade Off in Indian Stock Markets

    OpenAIRE

    Mohanty, Roshni; P, Srinivasan

    2014-01-01

    This paper investigates the relationship between stock market returns and volatility in the Indian stock markets using AR(1)-EGARCH(p, q)-in-Mean model. The study considers daily closing prices of two major indexes of Indian stock exchanges, viz., S&P CNX NIFTY and the BSE-SENSEX of National Stock Exchange (NSE) and Bombay Stock Exchange (BSE), respectively for the period from July 1, 1997 to December 31, 2013. The empirical results show positive but insignificant relationship between stock r...

  1. Neuroprotection of lamotrigine on hypoxic-ischemic brain damage in neonatal rats: Relations to administration time and doses

    Directory of Open Access Journals (Sweden)

    Yong-Hong Yi

    2008-06-01

    Full Text Available Yong-Hong Yi1, Wen-Chao Guo1, Wei-Wen Sun1, Tao Su1, Han Lin1, Sheng-Qiang Chen1, Wen-Yi Deng1, Wei Zhou2, Wei-Ping Liao11Department of Neurology, Institute of Neurosciences and the Second Affiliated Hospital, 2Department of Neonatology, Affiliated Guangzhou Children’s Hospital, Guangzhou Medical College, Guangzhou, Guangdong Province, P.R. ChinaAbstract: Lamotrigine (LTG, an antiepileptic drug, has been shown to be able to improve cerebral ischemic damage by limiting the presynaptic release of glutamate. The present study investigated further the neuroprotective effect of LTG on hypoxic-ischemic brain damage (HIBD in neonatal rats and its relations to administration time and doses. The HIBD model was produced in 7-days old SD rats by left common carotid artery ligation followed by 2 h hypoxic exposure (8% oxygen. LTG was administered intraperitoneally with the doses of 5, 10, 20, and 40 mg/kg 3 h after operation and the dose of 20 mg/kg 1 h before and 3 h, 6 h after operation. Blood and brain were sampled 24 h after operation. Nissl staining, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL, and neuron-specific enolase (NSE immunohistochemical staining were used for morphological studies. Water content in left cortex and NSE concentration in serum were determined. LTG significantly reduced water content in the cerebral cortex, as well as the number of TUNEL staining neurons in the dentate gyrus and cortex in hypoxic-ischemia (HI model. Furthermore, LTG significantly decreased the NSE level in serum and increased the number of NSE staining neurons in the cortex. These effects, except that on water content, were dose-dependent and were more remarkable in the pre-treated group than in the post-treated groups. These results demonstrate that LTG may have a neuroprotective effect on acute HIBD in neonates. The effect is more prominent when administrated with higher doses and before HI.Keywords: hypoxic-ischemic brain

  2. A Decision Support System (GesCoN for Managing Fertigation in Vegetable Crops. Part II – Model calibration and validation under different environmental growing conditions on field grown tomato

    Directory of Open Access Journals (Sweden)

    Giulia eConversa

    2015-07-01

    Full Text Available The GesCoN model was evaluated for its capability to simulate growth, nitrogen uptake and productivity of open field tomato grown under different environmental and cultural conditions. Five datasets collected from experimental trials carried out in Foggia (IT were used for calibration and 13 datasets collected from trials conducted in Foggia, Perugia (IT and Florida (USA were used for validation. The goodness of fitting was performed by comparing the observed and simulated shoot dry weight (SDW and N crop uptake during crop seasons, total dry weight (TDW, N uptake and fresh yield (TFY. In SDW model calibration, the relative RMSE values fell within the good 10 to 15% range, percent BIAS (PBIAS ranged between -11.5% and 7.4%. The Nash-Sutcliffe efficiency (NSE was very close to the optimal value 1. In the N uptake calibration RRMSE and PBIAS were very low(7%, and -1.78, respectively and NSE close to 1. The validation of SDW (RRMSE=16.7%; NSE=0.96 and N uptake (RRMSE=16.8%; NSE=0.96 showed the good accuracy of GesCoN. A model under- or overestimation of the SDW and N uptake occurred when higher or a lower N rates and/or a more or less efficient system were used compared to the calibration trial. The in-season adjustment, using the SDWcheck procedure, greatly improved model simulations both in the calibration and in the validation phases. The TFY prediction was quite good except in Florida, where a large overestimation (+16% was linked to a different harvest index (0.53 compared the cultivars used for model calibration and validation in Italian areas. The soil water content at the 10-30 cm depth appears to be well simulated by the software, and the GesCoN proved to be able to adaptively control potential yield and DW accumulation under limited N soil availability scenarios and consequently to modify fertilizer application. The DSSwell simulate SDW accumulation and N uptake of different tomato genotypes grown under Mediterranean and subtropical

  3. El compromiso y la confianza como factores incentivadores de la transmisión del conocimiento tácito dentro de la organización

    OpenAIRE

    Juárez Ibarra, Manuel; Trevilla Cantero, Celina

    2008-01-01

    La transmisión del conocimiento es un elemento clave para la generación de ventajas competitivas sostenibles, y dentro de la literatura se reconoce que la motivación extrínseca no es suficiente para lograr una transmisión voluntaria de conocimiento entre individuos y equipos de trabajo. En el presente artículo se destacan dos factores claves para conseguir que los empleados intercambien voluntariamente su conocimiento entre sí: el compromiso y la confianza –incluidos en la motivación intrínse...

  4. Neutron scattering. Experiment manuals

    International Nuclear Information System (INIS)

    Brueckel, Thomas; Heger, Gernot; Richter, Dieter; Roth, Georg; Zorn, Reiner

    2010-01-01

    The following topics are dealt with: The thermal triple axis spectrometer PUMA, the high-resolution powder diffractometer SPODI, the hot single-crystal diffractometer HEiDi for structure analysis with neutrons, the backscattering spectrometer SPHERES, neutron polarization analysis with tht time-of-flight spectrometer DNS, the neutron spin-echo spectrometer J-NSE, small-angle neutron scattering with the KWS-1 and KWS-2 diffractometers, the very-small-angle neutron scattering diffractrometer with focusing mirror KWS-3, the resonance spin-echo spectrometer RESEDA, the reflectometer TREFF, the time-of-flight spectrometer TOFTOF. (HSI)

  5. Effects of andrographolide on postoperative cognitive dysfunction and the association with NF-κB/MAPK pathway.

    Science.gov (United States)

    Ding, Yongbo; Shi, Cunxian; Chen, Linjing; Ma, Piliang; Li, Kezhong; Jin, Jin; Zhang, Qingfeng; Li, Aizhi

    2017-12-01

    The present study investigated the effects of andrographolide on postoperative cognitive dysfunction (POCD) in aged rats to gain insight of the underlying mechanism, which may provide theoretical basis for the clinical application of andrographolide to prevent POCD in older patients. Thirty aged male rats were randomly assigned to 3 groups: Control, model and andrographolide groups. The Morris water maze test was used to examine the spatial memory and learning ability of the rats postoperatively. The histological alterations of neuronal cells in the hippocampus were visualized by H&E staining. The serum levels of neuron-specific enolase (NSE), human soluble protein-100β (S-100β) and the inflammation factors of interluekin (IL)-1β, IL-6 and TNF-α involved in the nuclear factor κB (NF-κB)/mitogen-activated protein kinase (MAPK) signaling pathway were detected by ELISA. The NF-κB/MAPK signaling pathway-associated proteins in rat serum were detected by western blotting. Following andrographolide treatment, the rats significantly gained learning ability after surgery. Is it ameliorated hippocampal neuronal injury in rats following surgery. Andrographolide decreased NSE, S-100β, and the inflammation factors, IL-6, IL-1β and TNF-α in serum. Andrographolide reduced NF-κB/MAPK pathway-associated protein expression. Andrographolide ameliorated POCD in aged rats following surgery. The underlying mechanism may be associated with the downregulation the inflammatory factors and NF-κB/MAPK-associated protein expression.

  6. Longitudinal Nanotechnology Development (1991-2002): National Science Foundation Funding and its Impact on Patents

    International Nuclear Information System (INIS)

    Huang Zan; Chen Hsinchun; Yan Lijun; Roco, Mihail C.

    2005-01-01

    Nanotechnology holds the promise to revolutionize a wide range of products, processes and applications. It is recognized by over sixty countries as critical for their development at the beginning of the 21st century. A significant public investment of over $1 billion annually is devoted to nanotechnology research in the United States. This paper provides an analysis of the National Science Foundation (NSF) funding of nanoscale science and engineering (NSE) and its relationship to the innovation as reflected in the United States Patent and Trade Office (USPTO) patent data. Using a combination of bibliometric analysis and visualization tools, we have identified several general trends, the key players, and the evolution of technology topics in the NSF funding and commercial patenting activities. This study documents the rapid growth of innovation in the field of nanotechnology and its correlation to funding. Statistical analysis shows that the NSF-funded researchers and their patents have higher impact factors than other private and publicly funded reference groups. This suggests the importance of fundamental research on nanotechnology development. The number of cites per NSF-funded inventor is about 10 as compared to 2 for all inventors of NSE-related patents recorded at USPTO, and the corresponding Authority Score is 20 as compared to 1.8

  7. Longitudinal Nanotechnology Development (1991-2002): National Science Foundation Funding and its Impact on Patents

    Energy Technology Data Exchange (ETDEWEB)

    Huang Zan, E-mail: zhuang@eller.arizona.edu; Chen Hsinchun; Yan Lijun [University of Arizona, Department of Management Information Systems, Artificial Intelligence Lab, Eller College of Management (United States); Roco, Mihail C. [National Science Foundation (United States)

    2005-10-15

    Nanotechnology holds the promise to revolutionize a wide range of products, processes and applications. It is recognized by over sixty countries as critical for their development at the beginning of the 21st century. A significant public investment of over $1 billion annually is devoted to nanotechnology research in the United States. This paper provides an analysis of the National Science Foundation (NSF) funding of nanoscale science and engineering (NSE) and its relationship to the innovation as reflected in the United States Patent and Trade Office (USPTO) patent data. Using a combination of bibliometric analysis and visualization tools, we have identified several general trends, the key players, and the evolution of technology topics in the NSF funding and commercial patenting activities. This study documents the rapid growth of innovation in the field of nanotechnology and its correlation to funding. Statistical analysis shows that the NSF-funded researchers and their patents have higher impact factors than other private and publicly funded reference groups. This suggests the importance of fundamental research on nanotechnology development. The number of cites per NSF-funded inventor is about 10 as compared to 2 for all inventors of NSE-related patents recorded at USPTO, and the corresponding Authority Score is 20 as compared to 1.8.

  8. Parameter Optimisation and Uncertainty Analysis in Visual MODFLOW based Flow Model for predicting the groundwater head in an Eastern Indian Aquifer

    Science.gov (United States)

    Mohanty, B.; Jena, S.; Panda, R. K.

    2016-12-01

    The overexploitation of groundwater elicited in abandoning several shallow tube wells in the study Basin in Eastern India. For the sustainability of groundwater resources, basin-scale modelling of groundwater flow is indispensable for the effective planning and management of the water resources. The basic intent of this study is to develop a 3-D groundwater flow model of the study basin using the Visual MODFLOW Flex 2014.2 package and successfully calibrate and validate the model using 17 years of observed data. The sensitivity analysis was carried out to quantify the susceptibility of aquifer system to the river bank seepage, recharge from rainfall and agriculture practices, horizontal and vertical hydraulic conductivities, and specific yield. To quantify the impact of parameter uncertainties, Sequential Uncertainty Fitting Algorithm (SUFI-2) and Markov chain Monte Carlo (McMC) techniques were implemented. Results from the two techniques were compared and the advantages and disadvantages were analysed. Nash-Sutcliffe coefficient (NSE), Coefficient of Determination (R2), Mean Absolute Error (MAE), Mean Percent Deviation (Dv) and Root Mean Squared Error (RMSE) were adopted as criteria of model evaluation during calibration and validation of the developed model. NSE, R2, MAE, Dv and RMSE values for groundwater flow model during calibration and validation were in acceptable range. Also, the McMC technique was able to provide more reasonable results than SUFI-2. The calibrated and validated model will be useful to identify the aquifer properties, analyse the groundwater flow dynamics and the change in groundwater levels in future forecasts.

  9. Parameter Identification and Uncertainty Analysis for Visual MODFLOW based Groundwater Flow Model in a Small River Basin, Eastern India

    Science.gov (United States)

    Jena, S.

    2015-12-01

    The overexploitation of groundwater resulted in abandoning many shallow tube wells in the river Basin in Eastern India. For the sustainability of groundwater resources, basin-scale modelling of groundwater flow is essential for the efficient planning and management of the water resources. The main intent of this study is to develope a 3-D groundwater flow model of the study basin using the Visual MODFLOW package and successfully calibrate and validate it using 17 years of observed data. The sensitivity analysis was carried out to quantify the susceptibility of aquifer system to the river bank seepage, recharge from rainfall and agriculture practices, horizontal and vertical hydraulic conductivities, and specific yield. To quantify the impact of parameter uncertainties, Sequential Uncertainty Fitting Algorithm (SUFI-2) and Markov chain Monte Carlo (MCMC) techniques were implemented. Results from the two techniques were compared and the advantages and disadvantages were analysed. Nash-Sutcliffe coefficient (NSE) and coefficient of determination (R2) were adopted as two criteria during calibration and validation of the developed model. NSE and R2 values of groundwater flow model for calibration and validation periods were in acceptable range. Also, the MCMC technique was able to provide more reasonable results than SUFI-2. The calibrated and validated model will be useful to identify the aquifer properties, analyse the groundwater flow dynamics and the change in groundwater levels in future forecasts.

  10. Collective dynamics of glass-forming polymers at intermediate length scales

    International Nuclear Information System (INIS)

    Colmenero, J.; Alvarez, F.; Arbe, A.

    2015-01-01

    Deep understanding of the complex dynamics taking place in glass-forming systems could potentially be gained by exploiting the information provided by the collective response monitored by coherent neutron scattering. We have revisited the question of the characterization of the collective response of polyisobutylene at intermediate length scales observed by neutron spin echo (NSE) experiments. The model, generalized for sub-linear diffusion - as it is the case of glass-forming polymers - has been successfully applied by using the information on the total self-motions available from MD-simulations properly validated by direct comparison with experimental results. From the fits of the coherent NSE data, the collective time at Q → 0 has been extracted that agrees very well with compiled results from different experimental techniques directly accessing such relaxation time. We show that a unique temperature dependence governs both, the Q → 0 and Q → ∞ asymptotic characteristic times. The generalized model also gives account for the modulation of the apparent activation energy of the collective times with the static structure factor. It mainly results from changes of the short-range order at inter-molecular length scales

  11. Larmor-precession based neutron scattering instrumentation

    International Nuclear Information System (INIS)

    Ioffe, Alexander

    2009-01-01

    The Larmor precession of the neutron spin in a magnetic field allows the attachment of a Larmor clock to every neutron. Such Larmor labelling opens the possibility for the development of unusual neutron scattering techniques, where the energy (momentum) resolution does not require the initial and final states to be well selected. This principally allows for achievement of very high energy (momentum) resolution that is not feasible at all with conventional neutron scattering techniques, because the required neutron beam monochromatization (collimation) will result in intolerable intensity losses. Such decoupling of resolution and collimation allows, for example, for a significant increase in the luminosity of small-angle scattering or high-resolution diffractometers; the fact that opens new perspectives for their implementation at middle flux neutron sources. Different kinds of Larmor clock-based instrumentation, particularly two alternative NSE techniques using rotating and time-gradient magnetic field arrangements, which can be considered as inexpensive and affordable alternatives to present day NSE techniques, will be discussed and results of simulations and first experiments will be presented. (author)

  12. Perioperative erythropoietin protects the CNS against ischemic lesions in patients after open heart surgery.

    Science.gov (United States)

    Lakič, Nikola; Mrak, Miha; Šušteršič, Miha; Rakovec, Peter; Bunc, Matjaž

    2016-12-01

    The aim of this study was to establish erythropoietin as a protective factor against brain ischemia during open heart surgery. A total of 36 consecutive patients scheduled for revascularization heart surgery were included in the study. Of the patients 18 received 3 intravenous doses of recombinant human erythropoietin (rHuEpo, 24,000 IU) and 18 patients received a placebo. Magnetic resonance imaging (MRI) to detect new brain ischemic lesions was performed. Additionally, S100A, S100B, neuron-specific enolase A and B (NSE-A and B) and the concentration of antibodies against N‑methyl-D-aspartate receptors (NMDAR) to identify new neurological complications were determined. Patients who received rHuEpo showed no postoperative ischemic changes in the brain on MRI images. In the control group 5 (27.8 %) new ischemic lesions were found. The NMDAR antibody concentration, S100A, S100B and NSE showed no significant differences between the groups for new cerebral ischemia. High levels of lactate before and after external aortic compression (p = 0.022 and p = 0.048, respectively) and duration of operation could predict new ischemic lesions (p = 0.009). The addition of rHuEpo reduced the formation of lesions detectable by MRI in the brain and could be used clinically as neuroprotection in cardiac surgery.

  13. Experimental and numerical investigation of electrohydrodynamic flow in a point-to-ring corona discharge

    Science.gov (United States)

    Guan, Yifei; Vaddi, Ravi Sankar; Aliseda, Alberto; Novosselov, Igor

    2018-04-01

    An electrohydrodynamic (EHD) flow in a point-to-ring corona configuration is investigated experimentally and via a multiphysics computational model. The model couples the ion transport equation and the Navier-Stokes equations (NSE) to solve for the spatiotemporal distribution of electric field, flow field, and charge density. The numerical simulation results are validated against experimental measurements of the cathode voltage, ion concentration, and velocity profiles. The maximum flow velocity is at the centerline, and it decays rapidly with radial distance due to the viscous and electric forces acting on the partially ionized gas. To understand this coupling, a nondimensional parameter, X , is formulated as the ratio of the local electric force to the inertial term in the NSE. In the region of X ≥1 , the electric force dominates the flow dynamics, while in the X ≪1 region, the balance of viscous and inertial terms yields traditional pipe flow characteristics. This approach expands on the analytical model of Guan et al. by adding a description of the developing flow region. The approach allows the model to be used for the entire EHD domain, providing insights into the near-field flow in the corona region.

  14. Assessing Thermally Stressful Events in a Rhode Island Coldwater Fish Habitat Using the SWAT Model

    Directory of Open Access Journals (Sweden)

    Britta Chambers

    2017-09-01

    Full Text Available It has become increasingly important to recognize historical water quality trends so that the future impacts of climate change may be better understood. Climate studies have suggested that inland stream temperatures and average streamflow will increase over the next century in New England, thereby putting aquatic species sustained by coldwater habitats at risk. In this study we evaluated two different approaches for modeling historical streamflow and stream temperature in a Rhode Island, USA, watershed with the Soil and Water Assessment Tool (SWAT, using (i original SWAT and (ii SWAT plus a hydroclimatological model component that considers both hydrological inputs and air temperature. Based on daily calibration results with six years of measured streamflow and four years of stream temperature data, we examined occurrences of stressful conditions for brook trout (Salvelinus fontinalis using the hydroclimatological model. SWAT with the hydroclimatological component improved modestly during calibration (NSE of 0.93, R2 of 0.95 compared to the original SWAT (NSE of 0.83, R2 of 0.93. Between 1980–2009, the number of stressful events, a moment in time where high or low flows occur simultaneously with stream temperatures exceeding 21 °C, increased by 55% and average streamflow increased by 60%. This study supports using the hydroclimatological SWAT component and provides an example method for assessing stressful conditions in southern New England’s coldwater habitats.

  15. Assessing the Efficacy of the SWAT Auto-Irrigation Function to Simulate Irrigation, Evapotranspiration, and Crop Response to Management Strategies of the Texas High Plains

    Directory of Open Access Journals (Sweden)

    Yong Chen

    2017-07-01

    Full Text Available In the semi-arid Texas High Plains, the underlying Ogallala Aquifer is experiencing continuing decline due to long-term pumping for irrigation with limited recharge. Accurate simulation of irrigation and other associated water balance components are critical for meaningful evaluation of the effects of irrigation management strategies. Modelers often employ auto-irrigation functions within models such as the Soil and Water Assessment Tool (SWAT. However, some studies have raised concerns as to whether the function is able to adequately simulate representative irrigation practices. In this study, observations of climate, irrigation, evapotranspiration (ET, leaf area index (LAI, and crop yield derived from an irrigated lysimeter field at the USDA-ARS Conservation and Production Research Laboratory at Bushland, Texas were used to evaluate the efficacy of the SWAT auto-irrigation functions. Results indicated good agreement between simulated and observed daily ET during both model calibration (2001–2005 and validation (2006–2010 periods for the baseline scenario (Nash-Sutcliffe efficiency; NSE ≥ 0.80. The auto-irrigation scenarios resulted in reasonable ET simulations under all the thresholds of soil water deficit (SWD triggers as indicated by NSE values > 0.5. However, the auto-irrigation function did not adequately represent field practices, due to the continuation of irrigation after crop maturity and excessive irrigation when SWD triggers were less than the static irrigation amount.

  16. Diagnostic value of multi-tumor markers protein biochip detection for primary pulmonary cancer

    International Nuclear Information System (INIS)

    Xu Fengpo; Wu Yiwei; Li Qingru; Fa Yihua

    2005-01-01

    To evaluate the diagnostic value of multi-tumor markers protein biochip detection for primary pulmonary cancer, 12 tumor markers including AFP, CEA, NSE, CA125, CA15-3, CA242, CA19-9, PSA, f-PSA, FER, β-HCG and HGH were measured by the protein biochip in the serum of 45 primary pulmonary cancer patients. Positive rate of tumor markers was FER (42.2%), CEA (35.6%), CA125 (24.4%), CA15-3 (17.8%), CA242 (13.3%), CA19-9 (11.1%), β-HCG(8.9%), HGH(6.7%), NSE(4.4%), AFP (0), f-PSA (0) and PSA (0), respectively. The rate of patients with one abnorma indicator was 57.8% except FER. The positive rate using multi-tumor markers protein biochip detection was significantly higher than that of single tumor marker detective method, and this detection can be used for the diagnosis of patients with primary pulmonary cancer. (authors)

  17. Effect of PAS triple therapy on nerve injury, oxidative stress and inflammatory response in patients with cerebral infarction

    Directory of Open Access Journals (Sweden)

    Li-Jun Deng

    2017-10-01

    Full Text Available Objective: To study the effect of probucol + aspirin + atorvastatin (PAS triple therapy on nerve injury, oxidative stress and inflammatory response in patients with cerebral infarction. Methods: Patients with acute cerebral infarction who were treated in Affiliated Hospital of Jianghan University between February 2015 and January 2015 were selected and randomly divided into the PAS group who received probucol + aspirin + atorvastatin triple therapy and the control group who received aspirin + atorvastatin double therapy. The markers of nerve injury, oxidative stress and inflammatory response were determined before treatment and 15 d after treatment. Results: 15 d after treatment, peripheral blood Keap-1 expression and serum GPX1 contents of both groups of patients were significantly higher than those before treatment while peripheral blood Nrf-2 and ARE expression as well as serum S100B, NSE, sTRAIL, FKN, HMGB-1, sICAM-1, Chemerin and 8-iso-PGF2α contents were significantly lower than those before treatment, and peripheral blood Keap-1 expression and serum GPX1 content of PAS group were significantly higher than those of control group while peripheral blood Nrf-2 and ARE expression as well as serum S100B, NSE, sTRAIL, FKN, HMGB-1, sICAM-1, Chemerin and 8-iso-PGF2α contents were significantly lower than those of control group. Conclusion: PAS triple therapy can reduce the nerve injury as well as oxidative stress response and inflammatory response in patients with cerebral infarction.

  18. Assessment of adjuvant ademetionine therapy for the bilirubin metabolism and target organ function of neonatal jaundice

    Directory of Open Access Journals (Sweden)

    Fang Xu

    2017-11-01

    Full Text Available Objective: To study the effect of adjuvant ademetionine (SAMe therapy on the bilirubin metabolism and target organ function of neonatal jaundice. Methods: A total of 68 children who were diagnosed with neonatal jaundice in Hubei Jianghan Oilfield General Hospital between March 2015 and April 2017 were selected as the research subjects and randomly divided into the SAMe group who received ademetionine combined with blue ray irradiation and the control group who received blue ray irradiation. The serum contents of bilirubin metabolism indexes and target organ injury markers before treatment as well as 3 d and 7 d after treatment. Results: 3 d and 7 d after treatment, serum TBIL, ALT, AST, GGT, TBA, CK-MB, cTnT, MYO, HBDH, NSE, S100B and GFAP levels of both groups were lower than those before treatment, and serum TBIL, ALT, AST, GGT, TBA, CK-MB, cTnT, MYO, HBDH, NSE, S100B and GFAP levels of SAMe group were lower than those of control group. Conclusion: Adjuvant ademetionine therapy can improve the bilirubin metabolism of neonatal jaundice and reduce the central nerve, myocardial and liver injury.

  19. Correlation of serum MCP-1 and VE-cadherin levels with neural function and carotid atherosclerosis in patients with acute cerebral infarction

    Directory of Open Access Journals (Sweden)

    Yan-Bing Xi

    2017-05-01

    Full Text Available Objective: To study the correlation of serum monocyte chemoattractant protein-1 (MCP-1 and vascular endothelial cadherin (VE-cadherin levels with neural function and carotid atherosclerosis in patients with acute cerebral infarction. Methods: A total of 78 patients who were diagnosed with acute cerebral infarction in our hospital between May 2013 and August 2016 were selected as pathological group, and 80 healthy volunteers who received physical examination in our hospital during the same period were selected as control group. Serum was collected to determine the levels of MCP-1, VE-cadherin, nerve injury molecules, inflammatory mediators, proteases and their hydrolysate. Results: Serum MCP-1, VE-cadherin, NGB, NSE, S100β, HMGB-1, sCD40L, YKL-40, visfatin, CatK, MMP9 and ICTP levels of pathological group were significantly higher than those of control group; serum MCP-1 and VE-cadherin levels of pathological group were positively correlated with NGB, NSE, S100β, HMGB-1, sCD40L, YKL-40, visfatin, CatK, MMP9 and ICTP levels. Conclusion: Serum MCP-1 and VE-cadherin levels abnormally increase in patients with acute cerebral infarction, and are closely related to the nerve injury and atherosclerosis process.

  20. Pesticide transport simulation in a tropical catchment by SWAT

    International Nuclear Information System (INIS)

    Bannwarth, M.A.; Sangchan, W.; Hugenschmidt, C.; Lamers, M.; Ingwersen, J.; Ziegler, A.D.; Streck, T.

    2014-01-01

    The application of agrochemicals in Southeast Asia is increasing in rate, variety and toxicity with alarming speed. Understanding the behavior of these different contaminants within the environment require comprehensive monitoring programs as well as accurate simulations with hydrological models. We used the SWAT hydrological model to simulate the fate of three different pesticides, one of each usage type (herbicide, fungicide and insecticide) in a mountainous catchment in Northern Thailand. Three key parameters were identified: the sorption coefficient, the decay coefficient and the coefficient controlling pesticide percolation. We yielded satisfactory results simulating pesticide load dynamics during the calibration period (NSE: 0.92–0.67); the results during the validation period were also acceptable (NSE: 0.61–0.28). The results of this study are an important step in understanding the modeling behavior of these pesticides in SWAT and will help to identify thresholds of worst-case scenarios in order to assess the risk for the environment. - Highlights: • We performed a global LH-sensitivity analysis of all pesticide related parameters. • Key physical parameters are associated to percolation, degradation and sorption. • We simulated the measured loads of three different pesticides. • We performed an uncertainty analysis of all pesticide simulations. • All Pesticides differed considerably in their sensitivity and simulation behavior. - Pesticide load simulations of three pesticides were modeled by SWAT, providing clues on how to handle pesticides in future SWAT studies