Molecular constituents of the extracellular matrix in rat liver mounting a hepatic progenitor cell response for tissue repair

DEFF Research Database (Denmark)

Vestentoft, Peter Siig; Jelnes, Peter; Andersen, Jesper Bøje

2013-01-01

differentially up- or down-modulated (P tier of defense (Agrn, Bgn, Fbn1, Col4a1, Col8a1, Col9a3, Lama5, Lamb1, Lamb2, Itga4, Igtb2, Itgb4, Itgb6, Nid2), and their signal intensities showed a strong or very...... strong correlation with Krt1- 19, a well-established marker of a ductular/HPC reaction. Furthermore, a significant up-modulation and very strong correlation between the transcriptional profiles of Krt1-19 and St14 encoding matriptase, a component of a novel protease system, was found in the second tier...... that laminin, Collagen1a1, agrin and nidogen1 surrounded bile ducts, proliferating cholangiocytes, and HPCs in ductular reactions regardless of the nature of defense. Similarly, matriptase and HAI-1 were expressed in cholangiocytes regardless of the tier of defense, but in the second tier of defense...

Producción sostenible de cafetales en sistemas agroforestales del Neotrópico: una visión agrnómica y ecofisiológica

Directory of Open Access Journals (Sweden)

Fabio DaMatta

2007-06-01

Full Text Available Originario de regiones tropicales de África, el café evolucionó como especie leñosa del sub-bosque. Las primeras plantaciones de café fueron establecidas bajo condiciones de sombrío, en asocio con árboles de mayor altura con el fin de simular el hábitat natural del cafeto. Sin embargo, en muchas situaciones, las plantaciones de café a plena exposición presentan una productividad mayor que aquellas bajo sombrío; como consecuencia, el sombrío ha sido abandonado como una práctica cultural regular en muchas regiones productoras. Se ha discutido hace más de un siglo sí el sombrío favorece o no a las plantaciones de café en diferentes regiones productoras del mundo. En esta revisión se exploran y analizan aspectos agronómicos y eco-fisiológicos de la producción del cafeto bajo sombrío, en sistemas agro-forestales a plena exposición solar y, de manera particular, con Coffea arabica.

Identification and validation of genes involved in cervical tumourigenesis

International Nuclear Information System (INIS)

Rajkumar, Thangarajan; Sabitha, Kesavan; Vijayalakshmi, Neelakantan; Shirley, Sundersingh; Bose, Mayil Vahanan; Gopal, Gopisetty; Selvaluxmy, Ganesharaja

2011-01-01

Cervical cancer is the most common cancer among Indian women. This cancer has well defined pre-cancerous stages and evolves over 10-15 years or more. This study was undertaken to identify differentially expressed genes between normal, dysplastic and invasive cervical cancer. A total of 28 invasive cervical cancers, 4 CIN3/CIS, 4 CIN1/CIN2 and 5 Normal cervix samples were studied. We have used microarray technique followed by validation of the significant genes by relative quantitation using Taqman Low Density Array Real Time PCR. Immunohistochemistry was used to study the protein expression of MMP3, UBE2C and p16 in normal, dysplasia and cancers of the cervix. The effect of a dominant negative UBE2C on the growth of the SiHa cells was assessed using a MTT assay. Our study, for the first time, has identified 20 genes to be up-regulated and 14 down-regulated in cervical cancers and 5 up-regulated in CIN3. In addition, 26 genes identified by other studies, as to playing a role in cervical cancer, were also confirmed in our study. UBE2C, CCNB1, CCNB2, PLOD2, NUP210, MELK, CDC20 genes were overexpressed in tumours and in CIN3/CIS relative to both Normal and CIN1/CIN2, suggesting that they could have a role to play in the early phase of tumorigenesis. IL8, INDO, ISG15, ISG20, AGRN, DTXL, MMP1, MMP3, CCL18, TOP2A AND STAT1 were found to be upregulated in tumours. Using Immunohistochemistry, we showed over-expression of MMP3, UBE2C and p16 in cancers compared to normal cervical epithelium and varying grades of dysplasia. A dominant negative UBE2C was found to produce growth inhibition in SiHa cells, which over-expresses UBE2C 4 fold more than HEK293 cells. Several novel genes were found to be differentially expressed in cervical cancer. MMP3, UBE2C and p16 protein overexpression in cervical cancers was confirmed by immunohistochemistry. These will need to be validated further in a larger series of samples. UBE2C could be evaluated further to assess its potential as a

Canadian Food Irradiation Facilities; Installations Canadiennes d'Irradiation des Aliments; Kanadskie ustanovki dlya oblucheniya pishchevykh produktov; Instalaciones de Irradiacion de Alimentos en el Canada

Energy Technology Data Exchange (ETDEWEB)

Warland, H. M.F.; MacQueen, K. F. [Atomic Energy of Canada Ltd., Commercial Products, Ottawa (Canada)

1966-11-15

irradiacion, ya de por si sumamente util; se aumento la potencia de su fuente y se le doto de un sistema de refrigeracion de productos y de acondicionamiento de aire. Una vez modificado el dispositivo se utilizo en California para irradiar una gran variedad de frutas en la estacion que el Departamento de Agricultura de los Estados Unidos tiene en Fresno. En la memoria se describen en detalle la fuente tipo Gammacell, el dispositivo movil de irradiacion, la.instalacion con camara blindada, el dispositivo de irradiacion comercial de alimentos y algunos de los principales programas. El interes de la industria por la irradiacion de alimentos aumenta constantemente y las perspectivas en lo que respecta a nuevas instalaciones de irradiacion son muy alentadoras. (author) [Russian] V 1956 godu Kanadskoe akcionernoe obshhestvo po atomnoj jenergii (KAOAJe) nachalo obluchat' kartofel', primeniv otrabotannye toplivnye sterzhni v kachestve istochnika izluchenij. V 1958 godu KAOAJe skonstruirovalo i izgotovilo pervuju gamma-kameru 220 - avtonomnyj obluchatel', i kobal't-60 zatem ispol'zovalsja iskljuchitel'no v ramkah programmy issledovanij po oblucheniju pishhevyh produktov. V 1960 godu Upravlenie pishhevyh produktov i lekarstvennyh veshhestv vpervye razreshilo obluchat' kartofel'. Sledujushhaja stadija zakljuchalas' v tom, chtoby dokazat' proizvoditeljam kartofelja, chto kobal't-60 javljaetsja bezopasnym, prostym i nadezhnym sredstvom i chto obluchenie zaderzhit prorastanie v polevyh uslovijah. Zatem KAOAJe skonstruirovalo i izgotovilo peredvizhnoj obluchatel' v celjah osushhestvlenija programmy sozdanija opytnoj ustanovki. Obluchatel' byl ustanovlen pa polnost'ju oborudavannom avtomobil'nom pricepe i ispol'zovalsja vo vremja sezona 1961-1962 g. Im bylo obrabotano 450 t kartofelja v razlichnyh mestah Vostochnoj Kanady. V 1965 godu KAOAJe skonstruirovalo i izgotovilo pervyj promyshlennyj obluchatel' pishhevyh produktov dlja kompanii 'N'jufild Prodakts Limited. V hode osushhestvlenija