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Sample records for nonproteolytic prothrombin prot

  1. Genetics Home Reference: prothrombin thrombophilia

    Science.gov (United States)

    ... Understand Genetics Home Health Conditions prothrombin thrombophilia prothrombin thrombophilia Enable Javascript to view the expand/collapse boxes. Download PDF Open All Close All Description Prothrombin thrombophilia is an inherited disorder of blood clotting . Thrombophilia ...

  2. Prothrombin time (PT)

    Science.gov (United States)

    PT; Pro-time; Anticoagulant-prothrombin time; Clotting time: protime; INR; International normalized ratio ... PT is measured in seconds. Most of the time, results are given as what is called INR ( ...

  3. Prothrombin Segovia: a new congenital abnormality of prothrombin.

    Science.gov (United States)

    Rocha, E; Paramo, J A; Bascones, C; Fisac, P R; Cuesta, B; Fernandez, J

    1986-05-01

    A family with a new congenital dysprothrombinemia is presented. The propositus is a 21-yr-old man who presented simultaneously with hemartrosis of the left knee and an extensive hematoma following a minor trauma. Prothrombin time and activated partial thromboplastin time were prolonged. Prothrombin activity was very low when measured by biological assay using physiological activators (7% by one-stage method and 20% by two-stage method) or a Russel's viper venom-cephalin mixture (23%), Notechis scutatus scutatus venom (15%) and Echis carinatus venom (17%); in contrast, the level was found to be borderline to normal using Taipan viper venom (64%) and normal by both staphylocoagulase and immunologic methods. Family studies revealed consanguinity between the propositus' mother and father and both presented a 50% reduced prothrombin level when physiological activators or Echis carinatus viper venom were used. A line of identity between normal and abnormal prothrombin was observed on immunodiffusion. The migration of the abnormal prothrombin was less anodic and was not changed by the addition of calcium. The patient's serum showed 3 bands in the bidimensional immunoelectrophoresis system, whereas normal serum showed only 2 bands. The term prothrombin Segovia is proposed to define this new prothrombin abnormality.

  4. Quantification of Nonproteolytic Clostridium botulinum Spore Loads in Food Materials.

    Science.gov (United States)

    Barker, Gary C; Malakar, Pradeep K; Plowman, June; Peck, Michael W

    2016-01-04

    We have produced data and developed analysis to build representations for the concentration of spores of nonproteolytic Clostridium botulinum in materials that are used during the manufacture of minimally processed chilled foods in the United Kingdom. Food materials are categorized into homogenous groups which include meat, fish, shellfish, cereals, fresh plant material, dairy liquid, dairy nonliquid, mushroom and fungi, and dried herbs and spices. Models are constructed in a Bayesian framework and represent a combination of information from a literature survey of spore loads from positive-control experiments that establish a detection limit and from dedicated microbiological tests for real food materials. The detection of nonproteolytic C. botulinum employed an optimized protocol that combines selective enrichment culture with multiplex PCR, and the majority of tests on food materials were negative. Posterior beliefs about spore loads center on a concentration range of 1 to 10 spores kg(-1). Posterior beliefs for larger spore loads were most significant for dried herbs and spices and were most sensitive to the detailed results from control experiments. Probability distributions for spore loads are represented in a convenient form that can be used for numerical analysis and risk assessments.

  5. Systematic Assessment of Nonproteolytic Clostridium botulinum Spores for Heat Resistance

    Science.gov (United States)

    Stringer, Sandra C.; Barker, Gary C.; Peck, Michael W.

    2016-01-01

    ABSTRACT Heat treatment is an important controlling factor that, in combination with other hurdles (e.g., pH, aw), is used to reduce numbers and prevent the growth of and associated neurotoxin formation by nonproteolytic C. botulinum in chilled foods. It is generally agreed that a heating process that reduces the spore concentration by a factor of 106 is an acceptable barrier in relation to this hazard. The purposes of the present study were to review the available data relating to heat resistance properties of nonproteolytic C. botulinum spores and to obtain an appropriate representation of parameter values suitable for use in quantitative microbial risk assessment. In total, 753 D values and 436 z values were extracted from the literature and reveal significant differences in spore heat resistance properties, particularly those corresponding to recovery in the presence or absence of lysozyme. A total of 503 D and 338 z values collected for heating temperatures at or below 83°C were used to obtain a probability distribution representing variability in spore heat resistance for strains recovered in media that did not contain lysozyme. IMPORTANCE In total, 753 D values and 436 z values extracted from literature sources reveal significant differences in spore heat resistance properties. On the basis of collected data, two z values have been identified, z = 7°C and z = 9°C, for spores recovered without and with lysozyme, respectively. The findings support the use of heat treatment at 90°C for 10 min to reduce the spore concentration by a factor of 106, providing that lysozyme is not present during recovery. This study indicates that greater heat treatment is required for food products containing lysozyme, and this might require consideration of alternative recommendation/guidance. In addition, the data set has been used to test hypotheses regarding the dependence of spore heat resistance on the toxin type and strain, on the heating technique used, and on the

  6. Evaluation of Prothrombin Time and Activated Partial ...

    African Journals Online (AJOL)

    abnormal PT and APTT in untreated patients with diabetes mellitus. Keywords: Diabetes, Prothrombine ... may in turn enhance cardiovascular risk by increasing the likely hood of .... normalizes PT levels in diabetic patients (Standl et al,. 1996).

  7. Oral contraceptives and the prothrombin time.

    Science.gov (United States)

    Pangrazzi, J; Roncaglioni, M C; Donati, M B

    1980-02-02

    Dr. De Teresa and others reported that mean prothrombin time ratio of 12 patients on long-term anticoagulation with warfarin was significantly higher when they were also taking oral contraceptives (OCs). A study of prothrombin complex activity was recently conducted in female rats treated with an estrogen-progestogen combination (lynestrenol 5 mg; mestranol 0.3 mg/kg body weight) which resulted in a 100% infertility in this species. After 1 treatment for only 1 estral cycle, OC-treated rats had a significantly longer Normotest clotting time (37.7+ or-0.5 sec) than control rats (31.0+or-0.4); the difference was even more notable after 10 cycles. Although this finding has not been reported in women on OCs, it may be that the estrogen-induced "lability" of the prothrombin complex occurs in humans only in special conditions, such as anticoagulation. Alternatively, liver dysfunction occurring among women on OCs may be responsible for reduced metabolism of warfarin, contributing to the effectiveness of the anticoagulation. Further pharmacology studies should be done to clarify the interaction between OCs and oral anticoagulants.

  8. 21 CFR 866.5735 - Prothrombin immunological test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Prothrombin immunological test system. 866.5735 Section 866.5735 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... vitro coagulation studies classified under § 864.5425 of this chapter or prothrombin time tests...

  9. 21 CFR 864.7720 - Prothrombin consumption test.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Prothrombin consumption test. 864.7720 Section 864.7720 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... consumption test. (a) Identification. A prothrombin consumption tests is a device that measures the patient's...

  10. Growth of and toxin production by nonproteolytic Clostridium botulinum in cooked puréed vegetables at refrigeration temperatures.

    Science.gov (United States)

    Carlin, F; Peck, M W

    1996-08-01

    Seven strains of nonproteolytic Clostridium botulinum (types B, E, and F) were each inoculated into a range of anaerobic cooked puréed vegetables. After incubation at 10 degrees C for 15 to 60 days, all seven strains formed toxin in mushrooms, five did so in broccoli, four did so in cauliflower, three did so in asparagus, and one did so in kale. Growth kinetics of nonproteolytic C. botulinum type B in cooked mushrooms, cauliflower, and potatoes were determined at 16, 10, 8, and 5 degrees C. Growth and toxin production occurred in cooked cauliflower and mushrooms at all temperatures and in potatoes at 16 and 8 degrees C. The C. botulinum neurotoxin was detected within 3 to 5 days at 16 degrees C, 11 to 13 days at 10 degrees C, 10 to 34 days at 8 degrees C, and 17 to 20 days at 5 degrees C.

  11. Migration of tumor cells and leukocytes in extracellular matrix : proteolytic and nonproteolytic strategies for overcoming tissue barriers

    OpenAIRE

    Wolf, Katarina

    2003-01-01

    The extracellular matrix within connective tissues represents a structural scaffold as well as a barrier for motile cells, such as invading tumor cells or passenger leukocytes. It remains unclear how different cell types utilize matrix-degrading enzymes for proteolytic migration strategies and, on the other hand, non-proteolytic strategies to overcome 3D fibrillar matrix networks. To monitor cell migration, a 3D collagen model in vitro or the mouse dermis in vivo were used, in combination wit...

  12. Insulin-degrading enzyme prevents α-synuclein fibril formation in a nonproteolytical manner.

    Science.gov (United States)

    Sharma, Sandeep K; Chorell, Erik; Steneberg, Pär; Vernersson-Lindahl, Emma; Edlund, Helena; Wittung-Stafshede, Pernilla

    2015-07-31

    The insulin-degrading enzyme (IDE) degrades amyloidogenic proteins such as Amyloid β (Αβ) and Islet Amyloid Polypeptide (IAPP), i.e. peptides associated with Alzheimer's disease and type 2 diabetes, respectively. In addition to the protease activity normally associated with IDE function an additional activity involving the formation of stable, irreversible complexes with both Αβ and α-synuclein, an amyloidogenic protein involved in Parkinson's disease, was recently proposed. Here, we have investigated the functional consequences of IDE-α-synuclein interactions in vitro. We demonstrate that IDE in a nonproteolytic manner and at sub-stoichiometric ratios efficiently inhibits α-synuclein fibril formation by binding to α-synuclein oligomers making them inert to amyloid formation. Moreover, we show that, within a defined range of α-synuclein concentrations, interaction with α-synuclein oligomers increases IDE's proteolytic activity on a fluorogenic substrate. We propose that the outcomes of IDE-α-synuclein interactions, i.e. protection against α-synuclein amyloid formation and stimulated IDE protease activity, may be protective in vivo.

  13. Does proximity to neighbours affect germination of spores of non-proteolytic Clostridium botulinum?

    Science.gov (United States)

    Webb, Martin D; Stringer, Sandra C; Le Marc, Yvan; Baranyi, József; Peck, Michael W

    2012-10-01

    It is recognised that inoculum size affects the rate and extent of bacterial spore germination. It has been proposed that this is due to spores interacting: molecules released from germinated spores trigger germination of dormant neighbours. This study investigated whether changes to the total number of spores in a system or proximity to other spores (local spore density) had a more significant effect on interaction between spores of non-proteolytic Clostridium botulinum strain Eklund 17B attached to defined areas of microscope slides. Both the number of spores attached to the slides and local spore density (number of spores per mm(2)) were varied by a factor of nine. Germination was observed microscopically at 15 °C for 8 h and the probability of, and time to, germination calculated from image analysis measurements. Statistical analysis revealed that the effect of total spore number on the probability of germination within 8 h was more significant than that of proximity to neighbours (local spore density); its influence on germination probability was approximately four-times greater. Total spore number had an even more significant affect on time to germination; it had a nine-fold greater influence than proximity to neighbours. The applied models provide a means to characterise, quantitatively, the effect of the total spore number on spore germination relative to the effect of proximity to neighbouring spores.

  14. Intrauterine expression of prothrombin in the sprague-dawley rat.

    Science.gov (United States)

    Phillippe, Mark; Wolff, David; Saunders, Trevania; Thomas, Leandra; Chapa, Jeffrey

    2002-01-01

    Thrombin appears to underlie myometrial contractions in response to intrauterine bleeding. In a similar fashion, thrombin generated within the uterus in the absence of active bleeding could also produce contractions. These studies sought to determine whether functionally active prothrombin is expressed in the pregnant and nonpregnant rat uterus. Uteri were obtained from proestrus/estrus and timed-pregnant Sprague-Dawley rats. Western blots were performed using antithrombin antibodies. Immunohistochemical studies were performed using the same antibodies along with the Vector Elite ABC kit. Qualitative reverse transcriptase-polymerase chain reaction studies were performed using rat prothrombin-specific oligonucleotide primers. In vitro uterine contraction studies were performed using Taipan snake venom (an exogenous prothrombinase) and components of the plasma prothrombinase complex (Factors Xa and V) with and without pretreatment with thrombin inhibitors (heparin or hirudin). The Western blots demonstrated prothrombin peptides in myometrial tissue from estrus and pregnant rats. The immunohistochemical studies confirmed prothrombin peptides in both the circular and longitudinal myometrium, along with the endometrium. The reverse transcriptase-polymerase chain reaction studies demonstrated prothrombin mRNA in the endometrium and placenta, but not in the myometrial smooth muscle. The Taipan snake venom stimulated a significant increase in contractions, which were suppressed by pretreatment with heparin and hirudin. The Factor Xa and V complex also significantly stimulated uterine contractions, which were likewise inhibited by hirudin. These studies provide evidence supporting the expression of functionally active prothrombin in the pregnant and nonpregnant rat uterus. Based on the presence of its mRNA, prothrombin appears to be synthesized in the endometrium and placenta; in contrast, the myometrial smooth-muscle cells appear to sequester preformed prothrombin. These

  15. Development and application of a new method for specific and sensitive enumeration of spores of nonproteolytic Clostridium botulinum types B, E, and F in foods and food materials.

    Science.gov (United States)

    Peck, Michael W; Plowman, June; Aldus, Clare F; Wyatt, Gary M; Izurieta, Walter Penaloza; Stringer, Sandra C; Barker, Gary C

    2010-10-01

    The highly potent botulinum neurotoxins are responsible for botulism, a severe neuroparalytic disease. Strains of nonproteolytic Clostridium botulinum form neurotoxins of types B, E, and F and are the main hazard associated with minimally heated refrigerated foods. Recent developments in quantitative microbiological risk assessment (QMRA) and food safety objectives (FSO) have made food safety more quantitative and include, as inputs, probability distributions for the contamination of food materials and foods. A new method that combines a selective enrichment culture with multiplex PCR has been developed and validated to enumerate specifically the spores of nonproteolytic C. botulinum. Key features of this new method include the following: (i) it is specific for nonproteolytic C. botulinum (and does not detect proteolytic C. botulinum), (ii) the detection limit has been determined for each food tested (using carefully structured control samples), and (iii) a low detection limit has been achieved by the use of selective enrichment and large test samples. The method has been used to enumerate spores of nonproteolytic C. botulinum in 637 samples of 19 food materials included in pasta-based minimally heated refrigerated foods and in 7 complete foods. A total of 32 samples (5 egg pastas and 27 scallops) contained spores of nonproteolytic C. botulinum type B or F. The majority of samples contained <100 spores/kg, but one sample of scallops contained 444 spores/kg. Nonproteolytic C. botulinum type E was not detected. Importantly, for QMRA and FSO, the construction of probability distributions will enable the frequency of packs containing particular levels of contamination to be determined.

  16. The Universal Protein Resource (UniProt)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. The UniProt databases are the UniProt Knowledgebase...

  17. Inability of non-proteolytic Clostridium botulinum to grow in mussels inoculated via immersion and packaged in high oxygen atmospheres.

    Science.gov (United States)

    Newell, Carter R; Doyle, Michael; Ma, Li

    2015-04-01

    A series of botulism challenge studies were conducted to determine if botulinum toxin would be produced in mussels (Mytilus edulis) inoculated with non-proteolytic Clostridium botulinum spores and held under modified atmosphere (MA) packaging conditions at normal (4 °C) and abusive (12 °C) temperatures. Spore mixtures of six strains of non-proteolytic C. botulinum were introduced into live mussels through immersion in a seawater solution with cultured algae. Mussels were packed in a commercial high-oxygen (60-65% O2) MA-package with a buffer, and also packed under a vacuum. Feeding live mussels cultured algae (10(4) cells/ml) with a C. botulinum spore suspension (10(3) spores/ml) in seawater at 4 °C for 6 h resulted in the uptake of spores into mussel tissue (500/g) and the mussel GI tract (100/g). Under all of the experimental conditions evaluated, none of the fresh mussels became toxic, even after spoilage and in the absence of oxygen. However, control samples using tuna or cooked mussel meats became toxic in the absence of oxygen. Botulinum toxin was not produced in fresh mussels packaged under the MA-packaging conditions evaluated, even at an abusive storage temperature (12 °C) for at least 12 days or at normal storage temperate (4 °C) for at least 21 days, which is beyond their shelf life.

  18. Low paediatric thrombin generation is caused by an attenuation of prothrombin conversion.

    Science.gov (United States)

    Kremers, Romy M W; Wagenvoord, Rob J; de Laat, H Bas; Monagle, Paul; Hemker, H Coenraad; Ignjatovic, Vera

    2016-06-01

    Thrombin generation (TG) is decreased in children. TG is determined by two underlying processes: the conversion of prothrombin to thrombin and the inactivation of thrombin. Therefore, lower TG capacity in children can either be caused by a reduction of prothrombin conversion, an increase of thrombin inactivation, or both. In 36 children and 8 adults, TG and the factors that determine thrombin inactivation (antithrombin, α2Macroglobulin (α2M) and fibrinogen) were measured. Prothrombin conversion, thrombin inhibitor complex formation, and the overall thrombin decay capacity were determined. In silico modelling was performed to determine the contribution prothrombin conversion and thrombin inactivation to deviant paediatric TG. Both the amount of prothrombin converted and the maximal prothrombin conversion rate are significantly reduced in children as compared to adults. This is partly due to the prothrombin levels being lower and partly to a lower prothrombin conversion rate. The overall thrombin decay capacity is not significantly different in children, but α2Macroglobulin plays a more important role than it does in adults. In silico experiments demonstrate that reduced prothrombin conversion and to a lesser extent elevated α2M levels provide an explanation for low TG in children. Young age has a dual effect on prothrombin conversion. Lower plasma prothrombin levels result in decreased prothrombin conversion but the rate of prothrombin conversion is also decreased, i. e. the development of prothrombinase is lower than in adults.

  19. Inhibition of growth of nonproteolytic Clostridium botulinum type B in sous vide cooked meat products is achieved by using thermal processing but not nisin.

    Science.gov (United States)

    Lindström, M; Mokkila, M; Skyttä, E; Hyytiä-Trees, E; Lähteenmäki, L; Hielm, S; Ahvenainen, R; Korkeala, H

    2001-06-01

    The safety of refrigerated processed foods of extended durability (REPFEDs) with respect to nonproteolytic Clostridium botulinum is under continuous evaluation. In the present study, mild (P7.0(85.0) values 0 to 2 min [P, pasteurization value; z-value 7.0 degrees C; reference temperature 85.0 degrees C]) and increased (P7.0(85.0) values 67 to 515 min) heat treatments were evaluated in relation to survival of nonproteolytic C. botulinum type B spores in sous vide processed ground beef and pork cubes. The use of two concentrations of nisin in inhibition of growth and toxin production by nonproteolytic C. botulinum in the same products was also evaluated. A total of 96 samples were heat processed and analyzed for C. botulinum by BoNT/B gene-specific polmerase chain reaction and for botulinum toxin by a mouse bioassay after storage of 14 to 28 days at 4 and 8 degrees C. Predictably, after mild processing all samples of both products showed botulinal growth, and one ground beef sample became toxic at 8 degrees C. The increased heat processing, equivalent to 67 min at 85 degrees C. resulted in growth but not toxin production of C. botulinum in one ground beef sample in 21 days at 8 degrees C: in the pork cube samples no growth was detected. The increased heating of both products resulted in higher sensory quality than the milder heat treatment. Nisin did not inhibit the growth of nonproteolytic C. botulinum in either product; growth was detected in both products at 4 and 8 degrees C, and ground beef became toxic with all nisin levels within 21 to 28 days at 8 degrees C. Aerobic and lactic acid bacterial counts were reduced by the addition of nisin at 4 degrees C. The study demonstrates that the mild processing temperatures commonly employed in sous vide technology do not eliminate nonproteolytic C. botulinum type B spores. The intensity of each heat treatment needs to be carefully evaluated individually for each product to ensure product safety in relation to

  20. Factor V Leiden and Prothrombin Mutations in South of Iran

    Directory of Open Access Journals (Sweden)

    M Karimi

    2007-01-01

    Full Text Available ABSTRACT: Introduction & Objective: Factor V Leiden and prothrombin mutation are not common but they are involved in pediatric thrombosis. The aim of this study was to evaluate the frequency of factor V Leiden & prohtrombin mutation in healthy population of Shiraz, south of Iran. Materials & Methods: In this cross-sectional study 195 healthy people (97 female and 98 male were randomly selected. Peripheral white blood cells obtained from 5 ml blood contained 1-2 mg/ml K2- EDTA. Genomic DNA extraction was performed following the protocol described by Miller et al. PCR amplification was carried out in 25μl reaction volume containing 0.5 units Taq polymerase, 200μM dNTP, 500 μM of each of the previously described primers. After initial denaturation, 35 cycles at 95◦c for 30s, and 72◦c for 20s and followed extention by 72 for 10 min were performed. About 10μl of PDR product was digested with MNI I or Mbo restriction enzymes. Results: In this study we determined factor V Leiden in 8 (4.1% and prothrombin mutation in 6 individual (3.07% of 198 cases in heterozygous form. No homozygous was seen for any of the mutations. Only one case presented a double heterozygous for factor V and prothrombin in this cohort. Conclusion: Several studies of factor V leiden and prothrombin mutations in the East of Asia showed the higher frequency of these mutations in Iran.

  1. Combined high pressure and thermal processing on inactivation of type E and nonproteolytic type B and F spores of Clostridium botulinum.

    Science.gov (United States)

    Skinner, Guy E; Marshall, Kristin M; Morrissey, Travis R; Loeza, Viviana; Patazca, Eduardo; Reddy, N Rukma; Larkin, John W

    2014-12-01

    The aim of this study was to determine the resistance of multiple strains of the three nonproteolytic types of Clostridium botulinum (seven strains of type E, eight of type B, and two of type F) spores exposed to combined high pressure and thermal processing. The resistance of spores suspended in N-(2-acetamido)-2-aminoethanesulfonic acid (ACES) buffer (0.05 M, pH 7) was determined at a process temperature of 80°C with high pressures of 600, 650, and 700 MPa using a laboratory-scale pressure test system. Spores of C. botulinum serotype E strains demonstrated less resistance than nonproteolytic spores of type B or F strains when processed at 80°C and 600 MPa for up to 15 min. All C. botulinum type E strains were reduced by . 6.0 log units within 5 min under these conditions. Among the nonproteolytic type B strains, KAP 9-B was the most resistant, resulting in reductions of 2.7, 5.3, and 5.5 log, coinciding with D-values of 7.7, 3.4, and 1.8 min at 80°C and 600, 650, and 700 MPa, respectively. Of the two nonproteolytic type F strains, 610F was the most resistant, showing 2.6-, 4.5-, and 5.3-log reductions with D-values of 8.9, 4.3, and 1.8 min at 80°C and 600, 650, and 700 MPa, respectively. Pulsed-field gel electrophoresis was performed to examine the genetic relatedness of strains tested and to determine if strains with similar banding patterns also exhibited similar D-values. No correlation between the genetic fingerprint of a particular strain and its resistance to high pressure processing was observed.

  2. Characterisation of non-toxigenic Clostridium spp. strains, to use as surrogates for non-proteolytic Clostridium botulinum in chilled food challenge testing.

    Science.gov (United States)

    Parker, M D; Barrett, P I; Shepherd, J; Price, L J; Bull, S D

    2015-01-01

    Under many of the conditions studied, a two-strain cocktail of non-toxigenic Clostridium spp. was found to be suitable as a surrogate for non-proteolytic Clostridium botulinum, and has the potential for use in chilled food challenge tests measuring growth. Non-toxigenic surrogates could also be used in thermal process screening studies. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Quantitative risk assessment for hazards that arise from non-proteolytic Clostridium botulinum in minimally processed chilled dairy-based foods.

    Science.gov (United States)

    Malakar, P K; Barker, G C; Peck, M W

    2011-04-01

    A modular process risk model has been constructed that describes the manufacture of dairy dessert products and hazards that arise from non-proteolytic Clostridium botulinum. The model describes batch manufacture and consumer storage of a family size generic dairy dessert but includes a realistic quantification that could apply to a specific food product. The dairy dessert sector is an expanding part of the UK market. The model includes modules that describe spore loads in raw materials, spore inactivation during thermal processing, volume partition and the population kinetics for non-proteolytic C. botulinum during sequential isothermal storage regimes. Where possible elements of uncertainty and variability are identified explicitly. The model is constructed as a belief network from published data and expert opinions. The model provides marginal probabilities, and associated sensitivities, for a range of endpoint measures centred on the toxicity of a single retail unit after an extended period of storage. The decimal reduction time for non-proteolytic C. botulinum spore populations at the highest (hold) temperature of the primary thermal process and the highest temperature experienced during poorly controlled (consumer) storage are dominant factors determining risks. Priorities for additional information to support risk assessments have been identified.

  4. Effect of acetylcysteine on prothrombin index in paracetamol poisoning without hepatocellular injury

    DEFF Research Database (Denmark)

    Schmidt, Lars E; Knudsen, Tore Tveit; Dalhoff, Kim

    2002-01-01

    Acetylcysteine treatment reduces liver damage after paracetamol overdose, but can affect the prothrombin index, which is used to assess the progress of overdose patients. We aimed to assess retrospectively the effect of intravenous acetylcysteine on the prothrombin index in patients with paraceta......Acetylcysteine treatment reduces liver damage after paracetamol overdose, but can affect the prothrombin index, which is used to assess the progress of overdose patients. We aimed to assess retrospectively the effect of intravenous acetylcysteine on the prothrombin index in patients...... with paracetamol poisoning without signs of hepatocellular injury. Prothrombin index had been recorded before, and serially during, acetylcysteine treatment in 87 patients. After initiation of treatment, prothrombin index decreased (mean 0.33, 95% CI 0.29-0.38) in all patients, and was strongly associated...... with the start of acetylcysteine infusion. In patients with uncomplicated paracetamol poisoning, a fall in this index might be misinterpreted as a sign of liver failure, leading to prolonged treatment time....

  5. Reversing Antisense Oligonucleotide Activity with a Sense Oligonucleotide Antidote: Proof of Concept Targeting Prothrombin.

    Science.gov (United States)

    Crosby, Jeff R; Zhao, Chenguang; Zhang, Hong; MacLeod, A Robert; Guo, Shuling; Monia, Brett P

    2015-12-01

    The tissue half-life of second-generation antisense oligonucleotide drugs (ASOs) is generally longer than traditional small molecule therapeutics. Thus, a strategy to reverse the activity of antisense drugs is warranted in certain settings. In this study, we describe a strategy employing the administration of a complementary sense oligonucleotide antidote (SOA). As a model system we have chosen to target the coagulation factor and antithrombotic drug target, prothrombin, to assess the feasibility of this approach. ASO targeting mouse prothrombin specifically suppressed >90% hepatic prothrombin mRNA levels and circulating prothrombin protein in mice. These effects were dose- and time-dependent, and as expected produced predictable increases in anticoagulation activity [prothrombin time/activated partial thromboplastin time (PT/aPTT)]. Treatment with prothrombin SOAs resulted in a dose-dependent reversal of ASO activity, as measured by a return in prothrombin mRNA levels and thrombin activity, and normalization of aPTT and PT. The antithrombotic activity of prothrombin ASOs was demonstrated in a FeCl3-induced thrombosis mouse model, and as predicted for this target, the doses required for antithrombotic activity were also associated with increased bleeding. Treatment with SOA was able to prevent prothrombin ASO-induced bleeding in a dose-dependent manner. These studies demonstrate for the first time the utility of SOAs to selectively and specifically reverse the intracellular effects of an antisense therapy.

  6. Blood splash in lambs-a preliminary study using the one-stage prothrombin time test.

    Science.gov (United States)

    Restall, D J

    1981-02-01

    Lambs from a flock in which a high incidence of blood splash had been detected were examined using the one-stage prothrombin test. For comparison lambs from a commercial slaughter line were also examined. All the affected lambs and 35·4% from the slaughter line had extended prothrombin times, and a relationship between extended prothrombin times and the occurrence of blood splash was established. Investigation of the pastures grazed by the affected flock showed the presence of coumarin producing plants and grasses. Some coumarin drugs prolong one-stage prothrombin times, and more importantly, induce capillary fragility, thus predisposing animals to blood splash.

  7. Abnormal plasma prothrombin (PIVKA-II) levels in hepatocellular carcinoma.

    Science.gov (United States)

    Kawaguchi, Y

    1989-05-01

    The concentration of abnormal prothrombin, or the protein induced by vitamin K absence or antagonist II (PIVKA-II) in 102 patients with hepatic disorders was measured by an enzyme immunoassay method. The concentration of PIVKA-II in the plasma was elevated in 11 out of 18 patients with hepatocellular carcinoma and also in a patient with hepatoblastoma. There was no correlation between serum alpha-fetoprotein and plasma PIVKA-II levels. The PIVKA-II level was normal in 11 patients who had metastatic carcinoma or cholangiocellular carcinoma. Moreover, benign diseases of the liver did not cause an elevation in PIVKA-II. PIVKA-II might be an useful marker of hepatocellular carcinoma because, like alpha-fetoprotein, its level changes in close relation to the effects of treatment.

  8. Prothrombin Complex Concentrate Reduces Blood Product Utilization in Heart Transplantation.

    Science.gov (United States)

    Enter, Daniel H; Zaki, Anthony L; Marsh, Megan; Cool, Nikki; Kruse, Jane; Li, Zhi; Andrei, Adin-Cristian; Iddriss, Adam; McCarthy, Patrick M; Malaisrie, S Chris; Anderson, Allen; Rich, Jonathan D; Pham, Duc Thinh

    2017-08-22

    Current practices for the reversal of warfarin prior to cardiac surgery include the use of vitamin K and fresh frozen plasma (FFP) to reduce the risk of bleeding. Although the 2010 International Society of Heart and Lung Transplantation guidelines acknowledge the use of PCC (Prothrombin Complex Concentrate), there is no clear consensus on its efficacy. The objective of this study was to assess the efficacy of 4-factor Prothrombin Complex Concentrate (4-F PCC) administration in patients requiring warfarin reversal prior to heart transplantation by determining blood product utilization perioperatively. Twenty-one patients who received 4-F PCC for warfarin reversal prior to heart transplantation were compared to a similar cohort of 39 patients who did not receive 4-F PCC from January 2011 to July 2015. Blood product utilization was collected retrospectively for the 24-hour preoperative, intraoperative, and 48-hour postoperative periods. Patients receiving 4-F PCC required fewer blood products in all 3 time periods. In the 24-hour preoperative period, 22 patients (56%) in the control group and 2 patients (10%) in the 4-F PCC groups received blood products (p<0.001). Intraoperatively, all patients received blood products. The 4-F PCC group required fewer units of packed red blood cells (median 3 vs. 7 units, p<0.001) and FFP (median 4 vs. 9 units, p<0.001). In the 48-hour postoperative period, 20 patients (51%) in the control group and 5 patients (24%) in the 4-F PCC group received blood products (p=0.04). 4-F PCC is associated with reduced blood product utilization 24 hours preoperatively and intraoperatively. Historically, the majority of patients require FFP for warfarin reversal preoperatively. In this single-center study, a significant reduction in the need for FFP was demonstrated with the use of 4-F PCC. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  9. MASP-1 Induced Clotting--The First Model of Prothrombin Activation by MASP-1.

    Directory of Open Access Journals (Sweden)

    Lorenz Jenny

    Full Text Available Mannan-binding lectin-associated serine protease-1 (MASP-1, a protein of the complement lectin pathway, resembles thrombin in terms of structural features and substrate specificity. Due to its interplay with several coagulation factors, it has the ability to induce fibrin clot formation independent of the usual coagulation activation pathways. We have recently shown that MASP-1 activates prothrombin and identified arginine (R 155, R271, and R393 as potential cleavage sites. FXa cleaves R320 instead of R393, and thrombin cleaves R155 and R284 in prothrombin. Here we have used three arginine-to-glutamine mutants of prothrombin, R271Q, R320Q, R393Q and the serine-to-alanine active site mutant S525A to investigate in detail the mechanism of MASP-1 mediated prothrombin activation. Prothrombin wildtype and mutants were digested with MASP-1 and the cleavage products were analysed by SDS-PAGE and N-terminal sequencing. A functional clotting assay was performed by thrombelastography. We have found that MASP-1 activates prothrombin via two simultaneous pathways, either cleaving at R271 or R393 first. Both pathways result in the formation of several active alternative thrombin species. Functional studies confirmed that both R393 and R320 are required for prothrombin activation by MASP-1, whereas R155 is not considered to be an important cleavage site in this process. In conclusion, we have described for the first time a detailed model of prothrombin activation by MASP-1.

  10. Prevalence of factor Ⅴ Leiden and prothrombin G20210A in patients with gastric cancer

    Institute of Scientific and Technical Information of China (English)

    Sandra Battistelli; Massimo Stefanoni; Alberto Genovese; Aurelio Vittoria; Roberto Cappelli; Franco Roviello

    2006-01-01

    AIM: To analyze the prevalence of the two commonest thrombophilic mutations, factor Ⅴ Leiden and prothrombin G20210A, in patients with gastric cancer.METHODS: One hundred and twenty-one patients with primary gastric carcinoma and 130 healthy subjects,comparable for age and sex, were investigated. Factor Ⅴ Leiden was detected by using polymerase chain reaction and restriction enzyme digestion, and prothrombin G20210A gene mutation by allele-specific PCR.RESULTS: Among the 121 cancer patients, factor Ⅴ Leiden was found in 4 cases (GA genotype: 3.3%) and prothrombin G20210A in 10 cases (GA genotype: 8.3%).Of the 130 control subjects, factor Ⅴ Leiden was detected in 6 cases (GA genotype: 4.6%) and prothrombin G20210A in 8 cases (GA genotype: 6.1%). No double heterozygous carriers of both mutations were found in either group. The prevalence of both factor Ⅴ Leiden and prothrombin G20210A variant was not statistically different between the cancer patients and the healthy subjects.CONCLUSION: Our study suggests that, in gastric cancer, the risk factors of thrombophilic cancer state are on acquired rather than on a genetic basis and that prothrombin G20210A does not seem to be a cofactor in gastric cancer pathogenesis.

  11. Cotiarinase is a novel prothrombin activator from the venom of Bothrops cotiara.

    Science.gov (United States)

    Kitano, Eduardo S; Garcia, Thalita C; Menezes, Milene C; Tashima, Alexandre K; Zelanis, André; Serrano, Solange M T

    2013-08-01

    Snake venom serine proteinases (SVSPs) may affect hemostatic pathways by specifically activating components involved in coagulation, fibrinolysis and platelet aggregation or by unspecific proteolytic degradation. In this study, we purified and characterized an SVSP from Bothrops cotiara venom, named cotiarinase, which generated thrombin upon incubation with prothrombin. Cotiarinase was isolated by a two-step procedure including gel-filtration and cation-exchange chromatographies and showed a single protein band with a molecular mass of 29 kDa by SDS-polyacrylamide gel electrophoresis under reducing conditions. Identification of cotiarinase by mass spectrometric analysis revealed peptides that matched sequences of viperid SVSPs. Cotiarinase did not show fibrinogen-clotting, platelet-aggregating, fibrinogenolytic and factor X activating activities. Upon incubation with prothrombin the generation of thrombin was detected using the peptide substrate d-Phe-Pip-Arg-pNA. Moreover, mass spectrometric identification of prothrombin fragments generated by cotiarinase in the absence of co-factors (phospholipids, factor Va, factor Xa and Ca(2+) ions), indicated the limited proteolysis of this protein to release prothrombin 1, fragment 1 and thrombin. Cotiarinase is a novel SVSP that acts on prothrombin to release active thrombin that does not match any group of the current classification of snake venom prothrombin activators.

  12. Deceptive prothrombin and activated partial thromboplastin times in alcoholic cirrhosis.

    Science.gov (United States)

    Sirikonda, P. R.; Spillert, C. R.; Koneru, B.; Ponnudurai, R.; Wilson, D. J.; Lazaro, E. J.

    1996-01-01

    It is believed that perioperative hemorrhage, in the hepatoportal area, results from a coagulopathy. This study determined if this could be quantitated by a modified recalcification time (MRT) test developed in our laboratory. Unlike prothrombin (PT) and activated partial thromboplastin times (APTT), the MRT is performed with whole blood to ensure the role of blood cells and chemicals (particularly tissue factor, a potent procoagulant) in the coagulation process. Candidates for liver transplantation (n = 11) were studied. Samples (5 mL) of citrated venous blood were obtained from the patients. Aliquots (1 mL) from these samples were divided into groups of vials labeled C, S, and E. Groups C and S received 20 microL saline and group E, 20 microL of saline containing 10 micrograms of Escherichia coli endotoxin (055: B5W). Vial C was incubated for 10 minutes and vials S and E for 120 minutes, all at 37 degrees C. Then, the MRT was determined on 300 microL of blood from each vial after adding 40 microL of 0.1M calcium chloride. Mean MRT values (minutes +/- standard deviation) for C (MRTC), for S (MRTS), and for E (MRTE) were compared with like values from healthy controls (n = 29). Despite prolonged PT and APTT values, MRT values were shortened in patients with cirrhosis. This hypercoagulability detected by the MRT exonerates a hemorrhagic coagulopathy and possibly implicates widened and thinned gaps in the walls of the portal venous tributaries as the cause of perioperative hemorrhage. PMID:8667440

  13. A prothrombin activator from Bothrops erythromelas (jararaca-da-seca) snake venom: characterization and molecular cloning.

    Science.gov (United States)

    Silva, Márcia B; Schattner, Mirta; Ramos, Celso R R; Junqueira-de-Azevedo, Inácio L M; Guarnieri, Míriam C; Lazzari, María A; Sampaio, Claudio A M; Pozner, Roberto G; Ventura, Janaina S; Ho, Paulo L; Chudzinski-Tavassi, Ana M

    2003-01-01

    A novel prothrombin activator enzyme, which we have named 'berythractivase', was isolated from Bothrops erythromelas (jararaca-da-seca) snake venom. Berythractivase was purified by a single cation-exchange-chromatography step on a Resource S (Amersham Biosciences) column. The overall purification (31-fold) indicates that berythractivase comprises about 5% of the crude venom. It is a single-chain protein with a molecular mass of 78 kDa. SDS/PAGE of prothrombin after activation by berythractivase showed fragment patterns similar to those generated by group A prothrombin activators, which convert prothrombin into meizothrombin, independent of the prothrombinase complex. Chelating agents, such as EDTA and o -phenanthroline, rapidly inhibited the enzymic activity of berythractivase, like a typical metalloproteinase. Human fibrinogen A alpha-chain was slowly digested only after longer incubation with berythractivase, and no effect on the beta- or gamma-chains was observed. Berythractivase was also capable of triggering endothelial proinflammatory and procoagulant cell responses. von Willebrand factor was released, and the surface expression of both intracellular adhesion molecule-1 and E-selectin was up-regulated by berythractivase in cultured human umbilical-vein endothelial cells. The complete berythractivase cDNA was cloned from a B. erythromelas venom-gland cDNA library. The cDNA sequence possesses 2330 bp and encodes a preproprotein with significant sequence similarity to many other mature metalloproteinases reported from snake venoms. Berythractivase contains metalloproteinase, desintegrin-like and cysteine-rich domains. However, berythractivase did not elicit any haemorrhagic response. These results show that, although the primary structure of berythractivase is related to that of snake-venom haemorrhagic metalloproteinases and functionally similar to group A prothrombin activators, it is a prothrombin activator devoid of haemorrhagic activity. This is a feature

  14. Frequency of Factor V Leiden and Prothrombin Polymorphism in South of Iran

    Directory of Open Access Journals (Sweden)

    Javad Dehbozorgian

    2009-06-01

    Full Text Available Normal hemostasis requires balanced regulation of prothromboticand antithrombotic factors. Inherited alteration of factor Vand prothrombin gene, the G20210A mutation, increases the resistanceof factor V to degradation and booster production ofprothrombin respectively. These alterations can increase hypercoagulabilityleading to thrombotic consequences. We aimed toassess the frequencies of these mutations in a group of the populationof southern Iran. In total, 198 healthy volunteers with theage range of 1-64 years were selected and screened for factor VLeiden and prothrombin mutations using polymerase chain reactionand restriction fragment length polymorphism techniques.The carrier frequencies for factor V Leiden and prothrombin mutationin the studied cohort were 4.1% and 3.07%, respectively.In the studied area, the allele frequency of factor V ishigher than the prothrombin G20210A mutation (0.0204 v0.0153. According to the data and Hardy-Weinberger equation,the total risk of thrombosis caused by homozygosity andheterozygosity of factor V Leiden, prothrombin G20210A mutationand compound heterozygosity of these mutations areabout 1 in 500 individuals.

  15. Anti-prothrombin (aPT) and anti-phosphatidylserine/prothrombin (aPS/PT) antibodies and the risk of thrombosis in the antiphospholipid syndrome. A systematic review.

    Science.gov (United States)

    Sciascia, Savino; Sanna, Giovanni; Murru, Veronica; Roccatello, Dario; Khamashta, Munther A; Bertolaccini, Maria Laura

    2014-02-01

    Antibodies to prothrombin are detected by directly coating prothrombin on irradiated ELISA plates (aPT) or by using the phosphatidylserine/prothrombin complex as antigen (aPS/PT). Although these antibodies have both been associated with antiphospholipid syndrome (APS) and a correlation between the two assays have been reported, it seems that aPT and aPS/PT belong to different populations of autoantibodies. It was our objective to systematically review the available evidence on aPT and aPS/PT antibodies and the risk of thrombosis in APS. Medline-reports published between 1988 and 2013 investigating aPT and aPS/PT as a risk factor for thrombosis were included. Whenever possible, antibody isotype(s) and site of thrombosis were analysed. This systematic review is based on available data from more than 7,000 patients and controls from 38 studies analysing aPT and 10 aPS/PT. Antibodies to prothrombin (both aPT and aPS/PT) increased the risk of thrombosis (odds ratio [OR] 2.3; 95% confidence interval [CI] 1.72-3.5). aPS/PT seemed to represent a stronger risk factor for thrombosis, both arterial and/or venous than aPT (OR 5.11; 95%CI 4.2-6.3 and OR 1.82; 95%CI 1.44-2.75, respectively). In conclusion, routine measurement of aPS/PT (but not aPT) might be useful in establishing the thrombotic risk of patients with previous thrombosis and/or systemic lupus erythematosus. Their inclusion as laboratory criteria for the APS should be indisputably further explored.

  16. Successful living donor kidney transplantation in a patient with prothrombin gene mutation: Case report and literature review

    Directory of Open Access Journals (Sweden)

    Edward Shen

    2014-01-01

    Full Text Available We present a patient with known prothrombin gene mutation and a history of prior vascular events, who underwent living donor kidney transplantation. Given the presumed elevated risk of complication from known prothrombin mutation, clinical management was directed towards optimizing living donor allograft function.

  17. Mortality in vitamin K antagonist-related intracerebral bleeding treated with plasma or 4-factor prothrombin complex concentrate

    NARCIS (Netherlands)

    Majeed, Ammar; Meijer, Karina; Larrazabal, Ramiro; Arnberg, Fabian; Luijckx, Gert J.; Roberts, Robin S.; Schulman, Sam

    2014-01-01

    Prothrombin complex concentrates (PCC) can rapidly normalise prolonged prothrombin time, induced by vitamin K antagonists (VKA). We conducted a multicentre retrospective study to investigate whether reversal of VKA coagulopathy with 4-factor PCC improves the survival of patients with VKA-related int

  18. Laser-induced europium(III) luminescence as a probe of the metal ion mediated association of human prothrombin with phospholipid.

    Science.gov (United States)

    Rhee, M J; Horrocks, W D; Kosow, D P

    1982-09-14

    7F0 leads to 5D0 excitation spectroscopy of Eu(III) has been used to investigate the Eu(III) and phospholipid binding properties of human prothrombin. The results indicate that human prothrombin contains four high-affinity Eu(III) binding sites which are distributed into two classes of binding sites. When 4 equiv of Eu(III) is bound to prothrombin, the prothrombin is capable of binding to phospholipid vesicles. The deuterium isotope effect on the lifetime of the Eu(III)-prothrombin complex and the Eu(III)-prothrombin-phospholipid complex was used to determine the number of water molecules coordinated to the Eu(III). In both complexes, each of the Eu(III)'s coordinated to 2.5 +/- 0.5 water molecules. These results indicate that the binding of the Eu(III)-prothrombin complex to the phospholipid does not require the formation of a prothrombin-Eu(III)-phospholipid bridge.

  19. A simple method to discriminate between beta(2)-glycoprotein I- and prothrombin-dependent lupus anticoagulants

    NARCIS (Netherlands)

    Simmelink, MJA; Derksen, RHWM; Arnout, J; De Groot, PG

    2003-01-01

    Lupus anticoagulants (LAC) are a heterogeneous group of autoantibodies that prolong phospholipid-dependent clotting assays. The autoantibodies that cause LAC activity are predominantly directed against beta(2)-glycoprotein I (beta(2)GPI) or prothrombin. In the present study, we describe a method to

  20. Prothrombin complex concentrate in the reduction of blood loss during orthotopic liver transplantation : PROTON-trial

    NARCIS (Netherlands)

    Arshad, Freeha; Ickx, Brigitte; van Beem, Rachel T.; Polak, Wojciech; Grune, Frank; Nevens, Frederik; Ilmakunnas, Minna; Koivusalo, Anna-Maria; Isoniemi, Helena; Strengers, Paul F. W.; Groen, Henk; Hendriks, Herman G. D.; Lisman, Ton; Pirenne, Jacques; Porte, Robert J.

    2013-01-01

    Background: In patients with cirrhosis, the synthesis of coagulation factors can fall short, reflected by a prolonged prothrombin time. Although anticoagulants factors are decreased as well, blood loss during orthotopic liver transplantation can still be excessive. Blood loss during orthotopic liver

  1. Prothrombin complex concentrate in the reduction of blood loss during orthotopic liver transplantation: PROTON-trial

    NARCIS (Netherlands)

    F. Arshad (Freeha); B. Ickx (Brigitte); R.T. van Beem (Rachel); W.G. Polak (Wojciech); F. Grüne (Frank); F. Nevens (Frederik); M. Ilmakunnas (Minna); A.M. Koivusalo (Anna-Maria); H. Isoniemi (Helena); P.F.W. Strengers; H.J.M. Groen (Henk); H.G.D. Hendriks (Herman); T. Lisman (Ton); J. Pirenne (Jacques); R.J. Porte (Robert)

    2013-01-01

    textabstractBackground: In patients with cirrhosis, the synthesis of coagulation factors can fall short, reflected by a prolonged prothrombin time. Although anticoagulants factors are decreased as well, blood loss during orthotopic liver transplantation can still be excessive. Blood loss during

  2. Urinary prothrombin fragment 1+2 in patients with venous thrombosis and myocardial infarction

    NARCIS (Netherlands)

    Van Es, J.; Biere-Rafi, S.; Ahdi, M.; Kamphuisen, P.W.; Meijers, J.C.M.; Gerdes, V.E.A.

    2013-01-01

    Patients with venous-thromboembolism (VTE) and myocardial infarction (MI) have elevated prothrombin fragment 1+2 (F1+2) levels. In patients with postoperative VTE, urinary F1+2 (uF1+2) was higher than in individuals without VTE. To explore the relationship between plasma and uF1+2 we performed a pil

  3. A non-proteolytic role for ubiquitin in deadenylation of MHC-I mRNA by the RNA-binding E3-ligase MEX-3C

    Science.gov (United States)

    Cano, Florencia; Rapiteanu, Radu; Sebastiaan Winkler, G.; Lehner, Paul J.

    2015-01-01

    The regulation of protein and mRNA turnover is essential for many cellular processes. We recently showed that ubiquitin—traditionally linked to protein degradation—directly regulates the degradation of mRNAs through the action of a newly identified family of RNA-binding E3 ubiquitin ligases. How ubiquitin regulates mRNA decay remains unclear. Here, we identify a new role for ubiquitin in regulating deadenylation, the initial and often rate-limiting step in mRNA degradation. MEX-3C, a canonical member of this family of RNA-binding ubiquitin ligases, associates with the cytoplasmic deadenylation complexes and ubiquitinates CNOT7(Caf1), the main catalytic subunit of the CCR4-NOT deadenylation machinery. We establish a new role for ubiquitin in regulating MHC-I mRNA deadenylation as ubiquitination of CNOT7 by MEX-3C regulates its deadenylation activity and is required for MHC-I mRNA degradation. Since neither proteasome nor lysosome inhibitors rescued MEX-3C-mediated MHC-I mRNA degradation, our findings suggest a new non-proteolytic function for ubiquitin in the regulation of mRNA decay. PMID:26471122

  4. UniProt: the universal protein knowledgebase

    Science.gov (United States)

    2017-01-01

    The UniProt knowledgebase is a large resource of protein sequences and associated detailed annotation. The database contains over 60 million sequences, of which over half a million sequences have been curated by experts who critically review experimental and predicted data for each protein. The remainder are automatically annotated based on rule systems that rely on the expert curated knowledge. Since our last update in 2014, we have more than doubled the number of reference proteomes to 5631, giving a greater coverage of taxonomic diversity. We implemented a pipeline to remove redundant highly similar proteomes that were causing excessive redundancy in UniProt. The initial run of this pipeline reduced the number of sequences in UniProt by 47 million. For our users interested in the accessory proteomes, we have made available sets of pan proteome sequences that cover the diversity of sequences for each species that is found in its strains and sub-strains. To help interpretation of genomic variants, we provide tracks of detailed protein information for the major genome browsers. We provide a SPARQL endpoint that allows complex queries of the more than 22 billion triples of data in UniProt (http://sparql.uniprot.org/). UniProt resources can be accessed via the website at http://www.uniprot.org/. PMID:27899622

  5. A predictive model that describes the effect of prolonged heating at 70 to 90 degrees C and subsequent incubation at refrigeration temperatures on growth from spores and toxigenesis by nonproteolytic Clostridium botulinum in the presence of lysozyme.

    Science.gov (United States)

    Fernández, P S; Peck, M W

    1999-08-01

    Refrigerated processed foods of extended durability such as cook-chill and sous-vide foods rely on a minimal heat treatment at 70 to 95 degrees C and then storage at a refrigeration temperature for safety and preservation. These foods are not sterile and are intended to have an extended shelf life, often up to 42 days. The principal microbiological hazard in foods of this type is growth of and toxin production by nonproteolytic Clostridium botulinum. Lysozyme has been shown to increase the measured heat resistance of nonproteolytic C. botulinum spores. However, the heat treatment guidelines for prevention of risk of botulism in these products have not taken into consideration the effect of lysozyme, which can be present in many foods. In order to assess the botulism hazard, the effect of heat treatments at 70, 75, 80, 85, and 90 degrees C combined with refrigerated storage for up to 90 days on growth from 10(6) spores of nonproteolytic C. botulinum (types B, E, and F) in an anaerobic meat medium containing 2,400 U of lysozyme per ml (50 microg per ml) was studied. Provided that the storage temperature was no higher than 8 degrees C, the following heat treatments each prevented growth and toxin production during 90 days; 70 degrees C for >/=2,545 min, 75 degrees C for >/=463 min, 80 degrees C for >/=230 min, 85 degrees C for >/=84 min, and 90 degrees C for >/=33.5 min. A factorial experimental design allowed development of a predictive model that described the incubation time required before the first sample showed growth, as a function of heating temperature (70 to 90 degrees C), period of heat treatment (up to 2,545 min), and incubation temperature (5 to 25 degrees C). Predictions from the model provided a valid description of the data used to generate the model and agreed with observations made previously.

  6. An unusual cause of cerebral venous sinus thrombosis: prothrombin G20210A gene mutation.

    Science.gov (United States)

    Porres-Aguilar, Mateo; Square, Jaime H; Storey, Raul; Rodriguez-Dunn, Simon; Mohamed-Aly, Mohamed S

    2007-09-01

    Cerebral venous sinus thrombosis represents less than 1% of all strokes, being an uncommon entity with a wide spectrum of clinical scenarios. We present a 45-year-old Hispanic female with a history of long-term oral contraceptive use who was diagnosed with cerebral venous sinus thrombosis due to a heterozygous carrier mutation in the prothrombin G20210A gene. The patient was successfully managed with intravenous heparin with favorable clinical results without adverse effects. The prevalence of inherited primary thrombophilia increases with additional risk factors such as the use of oral contraceptives that can trigger or prothrombotic events in any vascular bed. An increased prevalence in the prothrombin G20210 gene mutation has been demonstrated in the Mexican-Mestizo population. Controversy exists regarding therapy of cerebral venous sinus thrombosis; according to experts, heparin remains the cornerstone of therapy with acceptable outcomes. More clinical trials are required to evaluate long-term outcomes in this subgroup of patients.

  7. Four methods compared for measuring des-carboxy-prothrombin (PIVKA-II).

    Science.gov (United States)

    Widdershoven, J; van Munster, P; De Abreu, R; Bosman, H; van Lith, T; van der Putten-van Meyel, M; Motohara, K; Matsuda, I

    1987-11-01

    PIVKA-II (Protein Induced by Vitamin K Absence) is abnormal des-carboxylated prothrombin, which is present in vitamin K deficiency or in patients using warfarin. With a sensitive method for PIVKA-II, biochemical vitamin K deficiency can be established before clinical symptoms occur. We give an overview of methods used to detect PIVKA-II, and four selected methods are inter-compared: (a) measuring total factor II including PIVKA-II by using Echis carinatus snake venom as an activator of prothrombin; (b) measuring PIVKA-II by using snake venom as an activator of factor II after adsorption of functional factor II onto barium sulfate; (c) electrophoresis-immunofixation method; and (d) enzyme immunoassay. We found d to be the most sensitive and reliable method for PIVKA-II.

  8. The influence of N-acetylcysteine on the measurement of prothrombin time and activated partial thromboplastin time in healthy subjects

    DEFF Research Database (Denmark)

    Jepsen, S; Hansen, A B

    1994-01-01

    The purpose of the study was to evaluate whether the infusion of N-acetylcysteine decreased the measurement of prothrombin time and activated partial thromboplastin time (APTT) in healthy persons. N-acetylcysteine was administered intraveneously 10 mg kg-1 as a loading dose and then at a rate of 10...... mg kg-1 h-1 for 32 h in six male subjects. The intrinsic, extrinsic and common pathway of coagulation were monitored with activated partial thromboplastin time (APTT), and prothrombin time, respectively. In addition, the extrinsic coagulation pathway was monitored with the clotting activity of single...... factors II, VII, and X. No effect on the intrinsic coagulation pathway was observed. There was a significant and rapid decrease in prothrombin time. Coagulation factors II, VII and X, the three components of prothrombin time, decreased significantly to different degrees. We conclude that infusion of N...

  9. Prealbumina, precalicreína e protrombina na forma hepatesplênica da esquistossomose: catabolismo aumentado de proteínas da coagulação? Prealbumin, prekallikrein and prothrombin in hepatosplenic schistosomiasis: increased turnover of the clotting proteins?

    Directory of Open Access Journals (Sweden)

    Nora Manoukian

    1984-10-01

    Full Text Available No plasma de 20 doentes portadores da forma hepatesplênica da esquistossomose mansônica, assim como no de 18 indivíduos normais, foram avaliados pré-albumina, precalicreína (atividade amidolítica e protrombina-antígeno. Os valores da concentração plasmática de pré-albumina obtidos no grupo controle permitiram que se estabelecesse um intervalo de referência (0,5 — 99,5% de 97 — 389 mg/I. Valores anormalmente diminuídos de pré-albumina encontrados em 2 dos doentes indicaram nestes uma disfunção hepatocítica de síntese protêica. Nos 18 doentes restantes a média da pré-albumina (232 ± 13 mg/l não diferiu (p > 0,05 da do grupo controle (243 ± 11 mg/l enquanto as médias da atividade de pró-calicreina (34 ± 1 μKat/l e protrombina (81 ± 3,0 mg/l estavam significativamente diminuídos no grupo de esquistossomóticos (p Prealbumin, prekallikrein and prothrombin were evaluated in 20 patients with the hepatosplenic form of schistosomiasis and in 18 subjects of a control group. With the prealbumin concentration values obtained in the latter group a reference interval (0.5 — 99.5% of 97 to 389 mg/L was calculated and plasma levels below 97 mg/L assessed in 2 patients a deficiency in hepatic synthetic capacity. Mean (± SEM prealbumin concentration in the 18 remaining patients (232 ± 13 mg/L did not differ (p > 0.05 from that of the control group (243 ± 11 mg/L. Otherwise these 18 patients had both mean plasma prekallikrein (34 ± 1.3 /μKat/L and prothrombin-antigen (81 ± 3 mg/L concentrations lower (p < 0.01 than those of the control group (40 ± 1.4 μKat/L and 100 ± 3 mg/L, respectively. The present findings do not necessarily rule out the possibility that prekallikrein and prothrombin may asses a minimal hepatocellular damage earlier than prealbumin; but, since the latter protein have a shorter half-life than the formers and is a reliable index of hepatic synthetic capacity, the present report supports the hypothesis

  10. Assessment of thrombogenicity of activated and non-activated prothrombin concentrates in a rat model.

    OpenAIRE

    Silberman, S.; Fareed, J; Walenga, J.

    1986-01-01

    In vitro clotting activity of rats injected with different preparations of prothrombin concentrates was measured. Animals rendered deficient in vitamin K-dependent coagulation factors by early coumadin (warfarin) pretreatment, followed by injections of concentrate preparations were also evaluated. Findings indicate a dose-related response in abnormal coagulation changes demonstrable with each preparation and lack of protection of intravascular coagulation by coumadin anticoagulation. Furtherm...

  11. Safety evaluation of sous vide-processed products with respect to nonproteolytic Clostridium botulinum by use of challenge studies and predictive microbiological models.

    Science.gov (United States)

    Hyytiä-Trees, E; Skyttä, E; Mokkila, M; Kinnunen, A; Lindström, M; Lähteenmäki, L; Ahvenainen, R; Korkeala, H

    2000-01-01

    Sixteen different types of sous vide-processed products were evaluated for safety with respect to nonproteolytic group II Clostridium botulinum by using challenge tests with low (2. 0-log-CFU/kg) and high (5.3-log-CFU/kg) inocula and two currently available predictive microbiological models, Food MicroModel (FMM) and Pathogen Modeling Program (PMP). After thermal processing, the products were stored at 4 and 8 degrees C and examined for the presence of botulinal spores and neurotoxin on the sell-by date and 7 days after the sell-by date. Most of the thermal processes were found to be inadequate for eliminating spores, even in low-inoculum samples. Only 2 of the 16 products were found to be negative for botulinal spores and neurotoxin at both sampling times. Two products at the high inoculum level showed toxigenesis during storage at 8 degrees C, one of them at the sell-by date. The predictions generated by both the FMM thermal death model and the FMM and PMP growth models were found to be inconsistent with the observed results in a majority of the challenges. The inaccurate predictions were caused by the limited number and range of the controlling factors in the models. Based on this study, it was concluded that the safety of sous vide products needs to be carefully evaluated product by product. Time-temperature combinations used in thermal treatments should be reevaluated to increase the efficiency of processing, and the use of additional antibotulinal hurdles, such as biopreservatives, should be assessed.

  12. The polyphenol-rich extract from grape seeds inhibits platelet signaling pathways triggered by both proteolytic and non-proteolytic agonists.

    Science.gov (United States)

    Olas, Beata; Wachowicz, Barbara; Stochmal, Anna; Oleszek, Wiesław

    2012-01-01

    Mechanisms involved in the reduction of blood platelet functions by various plant extract, including the grape seeds extract (rich in phenolic compounds, a mixture of about 95% oligomeric phenols; GSE) are still unclear. In the literature there are few papers describing studies on the effects of GSE on selected element of hemostasis. The aim of our study was to establish and compare the influence of GSE (at final dose of 0.625-50 µg/ml) and resveratrol (3,4',5 - trihydroxystilben), a phenolic compound synthesized in grapes and vegetables and presents in wine, which has been supposed to be beneficial for the prevention of cardiovascular events, on different steps of platelet activation. We measured the effects of GSE and resveratrol on platelet aggregation, the surface expression of P-selectin, platelet microparticle formation (PMP), and superoxide anion radicals ([Formula: see text]) production in blood platelets stimulated by TRAP and thrombin. P-selectin expression and PMP formation were measured by a flow cytometer. In gel-filtered platelets activated by thrombin or TRAP and treated with different concentrations of GSE (1.25-50 µg/ml) a significant decrease of P-selectin expression, PMP formation and platelet aggregation was observed. GSE caused also a dose-dependent reduction of [Formula: see text] produced in platelets activated by TRAP or thrombin. Our present results indicate that GSE inhibits platelet signaling pathways trigged by both proteolytic (thrombin) and non-proteolytic agonist (TRAP). In the comparative studies, GSE was found to be more effective antiplatelet factor, than the solution of pure resveratrol. Thus, the polyphenol-rich extract from grape seeds can be useful as the protecting factor against cardiovascular diseases.

  13. Prothrombin complex concentrate for reversal of vitamin K antagonist treatment in bleeding and non-bleeding patients

    DEFF Research Database (Denmark)

    Johansen, Mathias; Wikkelsø, Anne; Lunde, Jens

    2015-01-01

    BACKGROUND: Treatment with vitamin K antagonists is associated with increased morbidity and mortality. Reversal therapy with prothrombin complex concentrate (PCC) is used increasingly and is recommended in the treatment of patients with bleeding complications undertaking surgical interventions...... of prothrombin complex concentrate does not appear to reduce mortality or transfusion requirements but demonstrates the possibility of reversing vitamin K-induced coagulopathy without the need for transfusion of fresh frozen plasma. All included trials have high risk of bias and are underpowered to detect...

  14. The role of prothrombin complex concentrates in reversal of target specific anticoagulants.

    Science.gov (United States)

    Babilonia, Katrina; Trujillo, Toby

    2014-01-01

    Over the past several years a new era for patients requiring anticoagulation has arrived. The approval of new target specific oral anticoagulants offers practitioners several advantages over traditionally used vitamin K antagonist agents including predictable pharmacokinetics, rapid onset of action, comparable efficacy and safety, all without the need for routine monitoring. Despite these benefits, hemorrhagic complicates are inevitable with any anticoagulation treatment. One of the major disadvantages of the new oral anticoagulants is lack of specific antidotes or reversal agents for patients with serious bleeding or need for urgent surgery. As use of the new target specific oral anticoagulants continues to increase, practitioners will need to understand both the pharmacodynamics and pharmacokinetic properties of the agents, as well as, the available literature with use of non-specific therapies to reverse anticoagulation. Four factor prothrombin complex concentrates have been available for several years in Europe, and recently became available in the United States with approval of Kcentra. These products have shown efficacy in reversing anticoagulation from vitamin K antagonists, however their usefulness with the new target specific oral anticoagulants is poorly understood. This article will review the properties of dabigatran, rivaroxaban and apixaban, as well as the limited literature available on the effectiveness of prothrombin complex concentrates in reversal of their anticoagulant effects. Additional studies are needed to more accurately define the role of prothrombin complex concentrates in patients with life threatening bleeding or who require emergent surgery, as current data is both limited and conflicting.

  15. Acarboxy prothrombin (PIVKA II) as a tumour marker for hepatocellular carcinoma and other liver diseases.

    Science.gov (United States)

    Mohamedein, A; Yousif-Kadaru, A G; Ahmed, S A; Saida, H; Zaki, Z A; Eldin; Fedail, S S

    1995-09-01

    The clinical usefulness of plasma abnormal prothrombin, defined as protein induced by vitamin K absence or antagonist II: (PIVKA II) as a tumour marker for hepatocellular carcinoma (HCC) and other liver diseases has been evaluated. PIVKA II concentrations were determined using an enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody that reacts with PIVKA II but does not cross-react with normal prothrombin. Seventy four patients (74%) out of 100 with HCC had abnormal PIVKA II levels above 0.5 AU/ml (median = 3.4 AU/ml). The level was above 1.0 AU/ml in 66 (66%) of the patients. In contrast the level of PIVKA II was low in patients with bilharzial periportal fibrosis (median = 0.09 AU/ml), patients with liver cirrhosis (median = 0.13 AU/ml), patients with hepatitis (median = 0.025 AU/ml), and essentially undetectable in all the 34 controls. The diagnostic ability of serum alphafoetoprotein (AFP) was also evaluated in these patients. AFP alone can diagnose 51% of the HCC cases. Of the remaining patients with low or negative AFP levels (65%) can be diagnosed using PIVKA II. Abnormal prothrombin is a potential marker for the laboratory diagnosis of hepatocellular carcinoma.

  16. Purification and characterization of a prothrombin-activating protease from Nephila clavata.

    Science.gov (United States)

    Joo, Han-Seung; Park, Gun-Chun; Cho, Woo Ri; Tak, Eunsik; Paik, Seung R; Chang, Chung-Soon

    2002-03-01

    We report upon the purification and characterization of a novel prothrombin-activating enzyme from the body fluid (total homogenates of isolated digestive tract without eggs, spinnerets and silk glands) of the spider, Nephila clavata by a combination of acetone fractionation, ion exchange, and Soybean trypsin inhibitor-Sepharose chromatography. Analysis of the purified enzyme with SDS-PAGE and gel filtration revealed a single polypeptide chain with an apparent molecular weight of 24kDa. The proteolytic activity of the enzyme was stable up to 50 degrees C, however, it became unstable over 55 degrees C. The enzyme had an optimum pH of 8, and Ca(2+) was not required for the enzyme activity. According to inhibition profiles obtained with several serine protease inhibitors such as PMSF and benzamidine, the purified protease is a member of the serine proteases. Bz-Ile-Glu(gamma-OR)- Gly-Arg-pNA and Z-Arg-Gly-Arg-pNA which are known as substrates for factor Xa, were hydrolyzed favorably by the enzyme. And the Nephila protease could produce thrombin from prothrombin at nM range, and form the turbid ring using fibrinogen-agarose plate. The results obtained confirmed that the purified protease is a potent prothrombin-activating activity belonging to the family of serine protease.

  17. Increased prothrombin, apolipoprotein A-IV, and haptoglobin in the cerebrospinal fluid of patients with Huntington's disease.

    Directory of Open Access Journals (Sweden)

    Yen-Chu Huang

    Full Text Available Huntington's disease (HD is a progressive neurodegenerative disease caused by an unstable CAG trinucleotide repeat expansion. The need for biomarkers of onset and progression in HD is imperative, since currently reliable outcome measures are lacking. We used two-dimensional electrophoresis and mass spectrometry to analyze the proteome profiles in cerebrospinal fluid (CSF of 6 pairs of HD patients and controls. Prothrombin, apolipoprotein A-IV (Apo A-IV and haptoglobin were elevated in CSF of the HD patients in comparison with the controls. We used western blot as a semi-quantified measurement for prothrombin and Apo A-IV, as well as enzyme linked immunosorbent assay (ELISA for measurement of haptoglobin, in 9 HD patients and 9 controls. The albumin quotient (Qalb, a marker of blood-brain barrier (BBB function, was not different between the HD patients and the controls. The ratios of CSF prothrombin/albumin (prothrombin/Alb and Apo A-IV/albumin (Apo A-IV/Alb, and haptoglobin level were significantly elevated in HD. The ratio of CSF prothrombin/Alb significantly correlated with the disease severity assessed by Unified Huntington's Disease Rating Scale (UHDRS. The results implicate that increased CSF prothrombin, Apo A-IV, and haptoglobin may be involved in pathogenesis of HD and may serve as potential biomarkers for HD.

  18. Analytic validity of genetic tests to identify factor V Leiden and prothrombin G20210A.

    Science.gov (United States)

    Emadi, Ashkan; Crim, Matthew T; Brotman, Daniel J; Necochea, Alejandro J; Samal, Lipika; Wilson, Lisa M; Bass, Eric B; Segal, Jodi B

    2010-04-01

    The objective of this study is to systematically review methods for detecting Factor V Leiden or prothrombin G20210A. English-language literature from MEDLINE, EMBASE, The Cochrane Library, the Cumulative Index to Nursing and Allied Health Literature, PsycInfo(c), 2000-December 2008. Studies assessed methods for detection of these mutations in at least 10 human blood samples and reported concordance, discordance, or reproducibility. Two investigators abstracted data on the sample selection criteria, test operators, DNA extraction, experimental test, reference standard, commercial instruments, concordance rates, explanation of any discordance, and whether discordance resolved after repetition. We assessed strength of the evidence using the GRADE criteria. We reviewed 7,777 titles and included 66 articles. The majority of the reviewed studies used PCR-RFLP or AS-PCR as the reference standard. The studies demonstrated that commercially available and precommercial tests have high analytic validity with all having greater than 99% concordance with the reference standard. With a few exceptions, discordance resolved with repetition of the test, suggesting operator or administrative errors were responsible for the discordant results. In the quality assurance studies, greater than 98% of laboratories demonstrated high, even perfect, accuracy when asked to diagnose a sample with a known mutation. The majority of errors came from a limited number of laboratories. Although not all methods may be accurate, there is high-grade evidence that genetic tests for the detection of FVL and prothrombin G20210A have excellent analytic validity. There is high-grade evidence that most, but not all, clinical laboratories test for FVL and prothrombin G20210A accurately.

  19. Prothrombin-activator (thromboplastin) generation in the blood of water snake (Natrix piscator).

    Science.gov (United States)

    Srivastava, V M; Dube, B; Dube, R K; Agarwal, G P

    1981-12-23

    The generation of prothrombin-activator (thromboplastin) in water snake (Natrix piscator) is clearly delayed, compared to a mammalian system, but the final activity is well comparable to that in man, when homologous sources of "phospholipid" (erythrocyte-lysate) and of substrate plasma are employed in one stage "thromboplastin generation test". The use of heterologous source of either of the above reagents resulted in significantly longer clotting times; hence the need for homologous source of above reagents in the test is emphasized for comparative studies on animal haemostasis.

  20. Automatically extracting functionally equivalent proteins from SwissProt

    Directory of Open Access Journals (Sweden)

    Martin Andrew CR

    2008-10-01

    Full Text Available Abstract Background There is a frequent need to obtain sets of functionally equivalent homologous proteins (FEPs from different species. While it is usually the case that orthology implies functional equivalence, this is not always true; therefore datasets of orthologous proteins are not appropriate. The information relevant to extracting FEPs is contained in databanks such as UniProtKB/Swiss-Prot and a manual analysis of these data allow FEPs to be extracted on a one-off basis. However there has been no resource allowing the easy, automatic extraction of groups of FEPs – for example, all instances of protein C. We have developed FOSTA, an automatically generated database of FEPs annotated as having the same function in UniProtKB/Swiss-Prot which can be used for large-scale analysis. The method builds a candidate list of homologues and filters out functionally diverged proteins on the basis of functional annotations using a simple text mining approach. Results Large scale evaluation of our FEP extraction method is difficult as there is no gold-standard dataset against which the method can be benchmarked. However, a manual analysis of five protein families confirmed a high level of performance. A more extensive comparison with two manually verified functional equivalence datasets also demonstrated very good performance. Conclusion In summary, FOSTA provides an automated analysis of annotations in UniProtKB/Swiss-Prot to enable groups of proteins already annotated as functionally equivalent, to be extracted. Our results demonstrate that the vast majority of UniProtKB/Swiss-Prot functional annotations are of high quality, and that FOSTA can interpret annotations successfully. Where FOSTA is not successful, we are able to highlight inconsistencies in UniProtKB/Swiss-Prot annotation. Most of these would have presented equal difficulties for manual interpretation of annotations. We discuss limitations and possible future extensions to FOSTA, and

  1. A Predictive Model That Describes the Effect of Prolonged Heating at 70 to 90°C and Subsequent Incubation at Refrigeration Temperatures on Growth from Spores and Toxigenesis by Nonproteolytic Clostridium botulinum in the Presence of Lysozyme

    Science.gov (United States)

    Fernández, Pablo S.; Peck, Michael W.

    1999-01-01

    Refrigerated processed foods of extended durability such as cook-chill and sous-vide foods rely on a minimal heat treatment at 70 to 95°C and then storage at a refrigeration temperature for safety and preservation. These foods are not sterile and are intended to have an extended shelf life, often up to 42 days. The principal microbiological hazard in foods of this type is growth of and toxin production by nonproteolytic Clostridium botulinum. Lysozyme has been shown to increase the measured heat resistance of nonproteolytic C. botulinum spores. However, the heat treatment guidelines for prevention of risk of botulism in these products have not taken into consideration the effect of lysozyme, which can be present in many foods. In order to assess the botulism hazard, the effect of heat treatments at 70, 75, 80, 85, and 90°C combined with refrigerated storage for up to 90 days on growth from 106 spores of nonproteolytic C. botulinum (types B, E, and F) in an anaerobic meat medium containing 2,400 U of lysozyme per ml (50 μg per ml) was studied. Provided that the storage temperature was no higher than 8°C, the following heat treatments each prevented growth and toxin production during 90 days; 70°C for ≥2,545 min, 75°C for ≥463 min, 80°C for ≥230 min, 85°C for ≥84 min, and 90°C for ≥33.5 min. A factorial experimental design allowed development of a predictive model that described the incubation time required before the first sample showed growth, as a function of heating temperature (70 to 90°C), period of heat treatment (up to 2,545 min), and incubation temperature (5 to 25°C). Predictions from the model provided a valid description of the data used to generate the model and agreed with observations made previously. PMID:10427033

  2. Molecular characterization of factor V leiden G1691A and prothrombin G20210A mutations in Saudi newborns with stroke.

    Science.gov (United States)

    Gawish, Gihan E-H

    2011-10-01

    This study examined a possible association between the mutations related to Factor V Leiden and Factor II (prothrombin) and stroke in Saudi neonates. A multiplex PCR was established to detect Factor V Leiden G1691A and prothrombin G20210A mutations in 72 neonatal stroke subjects and 70 healthy adult controls with no family history of thromboembolic diseases. The frequency of the homozygous normal genotype (GG) of both genes was found to be significantly lower in the stroke subjects than in the controls (P Factor II heterozygous mutant form (GA) and the homozygous normal Factor V (GG) (P Factor V and the homozygous normal Factor II genotypes (GG) (P = 0.0) than controls. The study concluded that prothrombin and Factor V Leiden may be important risk factors for neonatal stroke in Saudi children.

  3. Different outcome of six homozygotes for prothrombin A20210A gene variant

    Directory of Open Access Journals (Sweden)

    Angiolillo Antonella

    2008-07-01

    Full Text Available Abstract Prothrombin G20210A gene variant (FII G20210A is a risk factor for venous thrombotic disease while conflicting results have been reported for the risk of arterial thrombotic events. However, vascular episodes were absent in up to 40% of the 67 homozygotes for the G20210A described so far, which indicates that the clinical expression depends on additional risk/trigger factors. We describe six homozygotes for the G20210A variant, among which the first pair of siblings (cases n. 3 and 4 reported so far that displayed a strongly heterogeneous clinical outcome. Case 1, a female of 27 years, developed a full thrombosis of common femoral, superficial and popliteal veins. She assumed oral contraceptives in the last two years. Case n. 2, 34 years old, suffered of recurrent pregnancy loss in absence of any causative alteration. Cases n. 3 and n. 5 experienced arterial thrombotic disease, i.e., juvenile myocardial infarction (40 years old and stroke (48 years old, respectively, in absence of other risk factors. Finally, cases n. 4 and 6 identified as homozygotes for the FII G20210A variant being consanguineous of symptomatic subjects bearing the variant, did not experience any episode of venous nor arterial disease. Both of them have chronic liver disease with an impairement of the prothrombin time INR. Thus, homozygotes for the G20210A are at risk for arterial (in addition to venous thromobotic events; chronic liver disease might modulate this risk.

  4. On the use of prothrombin complex concentrate in patients with coagulopathy requiring tooth extraction.

    Science.gov (United States)

    Morimoto, Yoshinari; Niwa, Hitoshi; Nakatani, Takeshi

    2010-12-01

    In patients on high-level anticoagulant therapy (prothrombin time-international normalized ratio [PT-INR] ≥ 4.5), surgical procedures can be carried out with bridging therapy using heparin. However, surgical treatment options are severely limited in patients on high-level anticoagulant therapy and who have heparin-induced thrombocytopenia (HIT), as heparin use is contraindicated. We performed tooth extraction using prothrombin complex concentrate (PCC) in 2 HIT patients on high-level anticoagulation therapy (PT-INR ≥ 4.5). Five hundred units of PCC were administered intravenously, and after 15 minutes, it was confirmed that PT-INR was less than 2.0. Tooth extraction was then performed and sufficient local hemostasis was achieved. At 3 hours after tooth extraction, PT-INR was 2.0 or higher and later increased to 4.0 or higher, but postoperative bleeding was mostly absent. When performing tooth extraction in HIT patients on high-level anticoagulant therapy, favorable hemostatic management was achieved through sufficient local hemostasis and transient warfarin reversal using PCC.

  5. A reduction of prothrombin conversion by cardiac surgery with cardiopulmonary bypass shifts the haemostatic balance towards bleeding.

    Science.gov (United States)

    Kremers, Romy M W; Bosch, Yvonne P J; Bloemen, Saartje; de Laat, Bas; Weerwind, Patrick W; Mochtar, Bas; Maessen, Jos G; Wagenvoord, Rob J; Al Dieri, Raed; Hemker, H Coenraad

    2016-08-30

    Cardiac surgery with cardiopulmonary bypass (CPB) is associated with blood loss and post-surgery thrombotic complications. The process of thrombin generation is disturbed during surgery with CPB because of haemodilution, coagulation factor consumption and heparin administration. We aimed to investigate the changes in thrombin generation during cardiac surgery and its underlying pro- and anticoagulant processes, and to explore the clinical consequences of these changes using in silico experimentation. Plasma was obtained from 29 patients undergoing surgery with CPB before heparinisation, after heparinisation, after haemodilution, and after protamine administration. Thrombin generation was measured and prothrombin conversion and thrombin inactivation were quantified. In silico experimentation was used to investigate the reaction of patients to the administration of procoagulant factors and/or anticoagulant factors. Surgery with CPB causes significant coagulation factor consumption and a reduction of thrombin generation. The total amount of prothrombin converted and the rate of prothrombin conversion decreased during surgery. As the surgery progressed, the relative contribution of α2-macroglobulin-dependent thrombin inhibition increased, at the expense of antithrombin-dependent inhibition. In silico restoration of post-surgical prothrombin conversion to pre-surgical levels increased thrombin generation excessively, whereas co-administration of antithrombin resulted in the normalisation of post-surgical thrombin generation. Thrombin generation is reduced during surgery with cardiopulmonary bypass because of a balance shift between prothrombin conversion and thrombin inactivation. According to in silico predictions of thrombin generation, this new balance increases the risk of thrombotic complications with prothrombin complex concentrate administration, but not if antithrombin is co-administered.

  6. Do Factor V Leiden and Prothrombin G20210A Mutations Predict Recurrent Venous Thromboembolism in Older Patients?

    Science.gov (United States)

    Méan, Marie; Limacher, Andreas; Stalder, Odile; Angelillo-Scherrer, Anne; Alberio, Lorenzo; Fontana, Pierre; Beer, Hans-Jürg; Rodondi, Nicolas; Lämmle, Bernhard; Aujesky, Drahomir

    2017-10-01

    The value of genetic thrombophilia testing in elderly patients with an unprovoked venous thromboembolism is unclear. We assessed whether the Factor V Leiden and the prothrombin G20210A mutation are associated with recurrent venous thromboembolism in elderly patients in a prospective multicenter cohort study. We genotyped the Factor V Leiden and the prothrombin G20210A mutation in 354 consecutive in- and outpatients aged ≥65 years with a first unprovoked venous thromboembolism from 9 Swiss hospitals. Patients and managing physicians were blinded to testing results. The outcome was recurrent symptomatic venous thromboembolism during follow-up. We examined the association between the Factor V Leiden and the prothrombin G20210A mutation and venous thromboembolism recurrence using competing risk regression, adjusting for age, sex, and periods of anticoagulation as a time-varying covariate. Overall, 9.0% of patients had a Factor V Leiden and 3.7% had a prothrombin G20210A mutation. At 36 months of follow-up, patients with a Factor V Leiden and a prothrombin G20210A mutation had a cumulative incidence of recurrent venous thromboembolism of 12.9% (95% confidence interval [CI], 5.1%-30.8%) and 18.5% (95% CI, 4.9%-56.5%), respectively, compared with 16.7% (95% CI, 12.5%-22.1%) of patients without mutation (P = .91 by the log-rank test). After adjustment, neither the Factor V Leiden (sub-hazard ratio 0.98; 95% CI, 0.35-2.77) nor the prothrombin G20210A mutation (sub-hazard ratio 1.15; 95% CI, 0.25-5.19) was associated with recurrent venous thromboembolism. Our results suggest that testing for genetic thrombophilia may not be beneficial in elderly patients with a first unprovoked venous thromboembolism. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. A Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) for the Detection of Factor V Leiden and Prothrombin G20210A.

    Science.gov (United States)

    Bagheri, Morteza; Rad, Isa Abdi

    2011-01-01

    In order to determine the frequencies of factor V Leiden and prothrombin G20210A point mutations in the Iranian population with Azeri Turkish origin. 120 unrelated individuals from general population randomly selected and were examined for factor V Leiden and prothrombin G20210A mutations using a multiplex allele specific polymerase chain reaction (MAS-PCR) assayOutcomes: The frequency of prothrombin G20210A mutation was 2.08%, which means 5 chromosomes out of 240 chromosomes had prothrombin G20210A mutation. The distribution of prothrombin 20210 GG, GA, AA genotypes and prothrombin 20210A allele were 37(92.5%), 3(7.5%), 0(0%) and 3(3.75%) in males and 78(97.5%), 2(2.5%), 0(0%) and 2(1.25%) in females, respectively. Factor V Leiden was not found in our tested group (zero chromosomes out of 240 chromosomes). Analysis of the observed frequencies in the studied groups indicates that there is no statistically significant difference between females and males, regarding prothrombin G20210A mutation (p value>0.05). This is the first study in its own kind in this population and implies that the frequency of Factor V Leiden G1691A (R506Q, FV-Leiden) allele is extremely low but the prothrombin G20210A mutation is more frequent in the tested group.

  8. Des-γ-Carboxy Prothrombin (DCP as a Potential Autologous Growth Factor for the Development of Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Yu-Sheng Zhang

    2014-08-01

    Full Text Available Des-γ-carboxy prothrombin (DCP is a prothrombin precursor produced in hepatocellular carcinoma (HCC. Because of deficiency of vitamin K or γ-glutamyl carboxylase in HCC cells, the 10 glutamic acid (Glu residues in prothrombin precursor did not completely carboxylate to γ-carboxylated glutamic acid (Gla residues, leaving some Glu residues remained in N-terminal domain. These prothrombin precursors with Glu residues are called DCPs. DCP displays insufficient coagulation activity. Since Liebman reported an elevated plasma DCP in patients with HCC, DCP has been used in the diagnosis of HCC. Recently, its biological malignant potential has been specified to describe DCP as an autologous growth factor to stimulate HCC growth and a paracrine factor to integrate HCC with vascular endothelial cells. DCP was found to stimulate HCC growth through activation of the DCP-Met-JAK1-STAT3 signaling pathway. DCP might increase HCC invasion and metastasis through activation of matrix metalloproteinase (MMPs and the ERK1/2 MAPK signaling pathway. DCP has also been found to play a crucial role in the formation of angiogenesis. DCP could increase the angiogenic factors released from HCC and vascular endothelial cells. These effects of DCP in angiogenesis might be related to activation of the DCP-KDR-PLC-γ-MAPK signaling pathway. In this article, we summarized recent studies on DCP in biological roles related to cancer progression and angiogenesis in HCC.

  9. Fresh frozen plasma versus prothrombin complex concentrate in patients with intracranial haemorrhage related to vitamin K antagonists (INCH)

    DEFF Research Database (Denmark)

    Steiner, Thorsten; Poli, Sven; Griebe, Martin

    2016-01-01

    of fresh frozen plasma (FFP) versus prothrombin complex concentrate (PCC) in patients with VKA-ICH. METHODS: We did an investigator-initiated, multicentre, prospective, randomised, open-label, blinded-endpoint trial. Patients aged at least 18 years with VKA-ICH who presented within 12 h after symptom onset...

  10. Use of Prothrombin Complex Concentrate in Patients during Heart Transplantation after Implantation of a Left Ventricular Mechanical Support System

    Directory of Open Access Journals (Sweden)

    V. V. Lomivorotov

    2012-01-01

    Full Text Available Heart transplantation in patients after implantation of mechanical cardiac support devices entails an extremely high risk for perioperative bleeding. Recombinant activated coagulation factor VII is presently used to reduce the volume of bleeding in this patient group. There are parallel data on its administration-induced thromboembolic events in the literature. This paper describes a case of using a prothrombin complex concentrate in a patient during explantation of a left ventricular bypass system and subsequent orthotopic heart transplantation in the presence of significant hypocoagulation. At the end of a surgery, 1200 IU of the agent was used at a remaining bleeding rate of more than 1000 ml/hour. Within the first 24 hours after surgery, the rate of discharge drainage was less than 100 ml/hour. A control plain chest X-ray study revealed massive left-sided hydrothorax on day 2 postsurgery. The left pleural cavity was revised under thoracoscopic guidance and 1000 ml of blood clots were evacuated. Although the administration of prothrombin complex concentrate did not guard against re-intervention, its use seems a promising strategy in life-threatening bleedings in patients after explantation of mechanical cardiac support devices. Further multicenter investigations are required to determine the efficacy and safety of prothrom-bin complex concentration in cardiac surgery. Key words: Recombinant activated coagulation factor VII, prothrombin complex concentration, mechanical cardiac support device, orthotopic heart transplantation.

  11. Des-γ-carboxy prothrombin (DCP) as a potential autologous growth factor for the development of hepatocellular carcinoma.

    Science.gov (United States)

    Zhang, Yu-Sheng; Chu, Jia-Hui; Cui, Shu-Xiang; Song, Zhi-Yu; Qu, Xian-Jun

    2014-01-01

    Des-γ-carboxy prothrombin (DCP) is a prothrombin precursor produced in hepatocellular carcinoma (HCC). Because of deficiency of vitamin K or γ-glutamyl carboxylase in HCC cells, the 10 glutamic acid (Glu) residues in prothrombin precursor did not completely carboxylate to γ-carboxylated glutamic acid (Gla) residues, leaving some Glu residues remained in N-terminal domain. These prothrombin precursors with Glu residues are called DCPs. DCP displays insufficient coagulation activity. Since Liebman reported an elevated plasma DCP in patients with HCC, DCP has been used in the diagnosis of HCC. Recently, its biological malignant potential has been specified to describe DCP as an autologous growth factor to stimulate HCC growth and a paracrine factor to integrate HCC with vascular endothelial cells. DCP was found to stimulate HCC growth through activation of the DCP-Met-JAK1-STAT3 signaling pathway. DCP might increase HCC invasion and metastasis through activation of matrix metalloproteinase (MMPs) and the ERK1/2 MAPK signaling pathway. DCP has also been found to play a crucial role in the formation of angiogenesis. DCP could increase the angiogenic factors released from HCC and vascular endothelial cells. These effects of DCP in angiogenesis might be related to activation of the DCP-KDR-PLC-γ-MAPK signaling pathway. In this article, we summarized recent studies on DCP in biological roles related to cancer progression and angiogenesis in HCC.

  12. Portal Vein Thrombosis due to Prothrombin Gene Mutation following Sleeve Gastrectomy

    Directory of Open Access Journals (Sweden)

    Murad Baba

    2015-01-01

    Full Text Available Introduction. Portomesenteric thrombosis is increasingly recognized as a complication of laparoscopic sleeve gastrectomy (LSG. It often presents with abdominal pain. We present a mother and her son who both developed portal vein thrombosis (PVT after LSG. Case Description. A 43-year-old woman presented complaining of sudden severe abdominal pain, two weeks after she had uncomplicated laparoscopic sleeve gastrectomy. CT scan of the abdomen and pelvis with IV contrast showed portal vein thrombosis and SMV thrombosis. Two weeks later her son had the same LSG for morbid obesity and presented with the same clinical picture. Thrombophilia workup showed heterozygous prothrombin gene mutation. Conclusions. A high index of suspicion is necessary to diagnose PVT; although rare, it can be potentially lethal. Anticoagulation therapy should be initiated immediately to limit the morbidities and improve the outcome. Patients with family history of thrombophilia should be investigated prior to any bariatric surgery and nonsurgical alternative treatments for morbid obesity should be strongly encouraged.

  13. Significance of Des-gamma-carboxy Prothrombin Production in Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Yamamoto,Kazuhide

    2009-12-01

    Full Text Available Serum des-gamma-carboxy prothrombin (DCP is commonly used to detect hepatocellular carcinoma (HCC. This review focuses on the clinical features of DCP-positive HCC and the molecular function of DCP in HCC. DCP-positive HCC demonstrates more aggressive clinicopathological features than DCP-negative HCC. Analysis of the biological effects of DCP revealed that DCP acts as a growth factor in both an autocrine and paracrine manner. DCP stimulates HCC cell proliferation through the Met-Janus kinase 1-signal transducer and activator of transcription 3 signaling pathway, whereas for vascular endothelial cells, it stimulates cell proliferation and migration through the kinase insert domain receptor-phospholipase C-gamma-mitogen-activated protein kinase signaling pathway.

  14. Acarboxy prothrombin (PIVKA-II) in cord plasma in the south of Thailand.

    Science.gov (United States)

    Laosombat, V; Kenpitak, K; Wongchanchailert, M; Wiriyasateinkul, A

    1993-05-01

    Acarboxy prothrombin or PIVKA-II (protein induced by vitamin K absence or antagonist-II) was used to determine the presence of vitamin deficiency in newborn infants. Of 230 cord blood samples assayed by using ELISA method, 34.8 per cent were positive for PIVKA-II 0.13-17 AU/ml. The positive rate for PIVKA-II was greater in infants of primigravida (50.7%) than in those of multigravida (27.9%). All infants received prophylactic vitamin K, and no infant with positive PIVKA-II in cord blood subsequently had clinical bleeding. Because of the high prevalence of vitamin K deficiency in newborn infants in the South of Thailand, all newborn infants should receive prophylactic vitamin K at birth.

  15. Acarboxy prothrombin (PIVKA-II) as a marker of hepatoblastoma in infants.

    Science.gov (United States)

    Motohara, K; Endo, F; Matsuda, I; Iwamasa, T

    1987-01-01

    We evaluated plasma PIVKA-II (protein induced by vitamin K absence or antagonist-II, acarboxy prothrombin) levels in three infants with hepatoblastoma as a tumor marker. PIVKA-II levels were highly elevated in all three patients. Vitamin K administration, performed in two patients, resulted in only moderate reduction of PIVKA-II levels. Chemotherapy against tumor cells reduced the PIVKA-II levels without exception. Immunohistochemical study of the liver tissue indicated the presence of PIVKA-II in the hepatoblastoma cell. These findings suggest that elevated PIVKA-II in these patients was not due to nutritional vitamin K deficiency, but to excess production of tumor cells. A measurement of plasma PIVKA-II may be useful as a new marker of hepatoblastoma.

  16. Prothrombin complex concentrate use in coagulopathy of lethal brain injuries increases organ donation.

    Science.gov (United States)

    Joseph, Bellal; Aziz, Hassan; Pandit, Viraj; Hays, Daniel; Kulvatunyou, Narong; Tang, Andrew; Wynne, Julie; O' Keeffe, Terence; Green, Donald J; Friese, Randall S; Gruessner, Rainer; Rhee, Peter

    2014-04-01

    Coagulopathy is a defined barrier for organ donation in patients with lethal traumatic brain injuries. The purpose of this study was to document our experience with the use of prothrombin complex concentrate (PCC) to facilitate organ donation in patients with lethal traumatic brain injuries. We performed a 4-year retrospective analysis of all patients with devastating gunshot wounds to the brain. The data were analyzed for demographics, change in international normalized ratio (INR), and subsequent organ donation. The primary end point was organ donation. Eighty-eight patients with lethal traumatic brain injury were identified from the trauma registry of whom 13 were coagulopathic at the time of admission (mean INR 2.2 ± 0.8). Of these 13 patients, 10 patients received PCC in an effort to reverse their coagulopathy. Mean INR before PCC administration was 2.01 ± 0.7 and 1.1 ± 0.7 after administration (P brain injuries.

  17. Magnetic resonance imaging of myocardial infarction during prothrombin complex concentrate therapy of hemophilia A

    Energy Technology Data Exchange (ETDEWEB)

    Gruen, D.R. [Dept. of Radiology, The New York Hospital-Cornell Medical Center, New York, NY (United States); Winchester, P.H. [Dept. of Radiology, The New York Hospital-Cornell Medical Center, New York, NY (United States); Brill, P.W. [Dept. of Radiology, The New York Hospital-Cornell Medical Center, New York, NY (United States); Ramirez, E. [Dept. of Radiology, The New York Hospital-Cornell Medical Center, New York, NY (United States)

    1997-03-01

    In patients with hemophilia, prothrombin complex concentrates (PCCs) have been successfully used to bypass inhibitors to fctor VIII during bleeding episodes. The use of PCCS, including FEIBA (factor eight inhibitor bypassing activity), has been associated with thromboembolic complications. Myocardial infarction (MI) is a rare but serious complication, reported in 13 previous cases, six in the pediatric age group. In all four patients who died during the acute MI, autopsy revealed extensive myocardial hemorrhage. The hearts of three other patients examined at least 5 months after the acute MI showed no evidence of prior hemorrhage. Magnetic resonance (MR) imaging has been shown to be able to evaluate the sequelae of myocardial infarction in adults with coronary artery disease and in children with Kawasaki syndrome. We report the first case of the used of MR imaging in the evaluation of myocardial damage during the acute stage of a FEIBA-associated MI in a 10-year-old boy. (orig.)

  18. Association between prothrombin gene polymorphisms and hereditary thrombophilia in Xinjiang Kazakhs population.

    Science.gov (United States)

    Ge, Xiao-Hu; Zhu, Feng; Wang, Bing-Lin; Wang, Chang-Min; Zhu, Bing; Guan, Sheng; Ci, Hong-Bo; Sai, Li-Mu; Jiang, Xiao-Kui; Ren, Hao; Fang, Qing-Bo; Tian, Guang-Lei

    2014-03-01

    To assess the association between polymorphisms of prothrombin gene and hereditary thrombophilia in Xinjiang Kazakhs population. Through cross-sectional investigation, permanent Kazakh population of Ili Kazakh Autonomous Prefecture was selected as the study object to measure their antithrombin III (AT-III), protein C, protein S activity and activated C protein resistance value, thus defining the situation of the crowd's hereditary thrombophilia. Sequenom Massarray detection technology was used to conduct a genotype test of the six sites selected by the case and control groups. Haploview software was used to perform linkage disequilibrium analysis of the six sites, and the impact of the interaction between genetic variations and environment on hereditary thrombophilia was researched by the use of sum model. A total of 1005 Kazakh volunteers participated in the test (332 men and 673 women), average age (41.13 ± 11.50) years; the prevalence of hereditary thrombophilia in Xinjiang Kazakh population was 31.0%, and the prevalence of AT-III deficiency, protein C deficiency, protein S deficiency and activated protein C resistance was 16.4, 14.9, 20.6 and 7.8%, respectively. The difference in allele frequency of the hereditary thrombophilia patient group at rs3136447 and rs5896 sites was statistically significant (P = 0.0483 and P = 0.0302, respectively). rs5896 and rs2070852 had high linkage disequilibrium (r = 0.99), and constituted a single-domain block 1. The rs3136447 and the rs5896 polymorphisms located in the region of the prothrombin gene may be associated with hereditary thrombophilia in the Xinjiang Kazakhs population. There is additive interactive effect of rs5896 polymorphism (CT + TT) and smoke on hereditary thrombophilia.

  19. Clinical factors influencing normalization of prothrombin time after stopping warfarin: a retrospective cohort study

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    Zondag Michelle

    2008-10-01

    Full Text Available Abstract Background Anticoagulation with warfarin should be stopped 4–6 days before invasive procedures to avoid bleeding complications. Despite this routine, some patients still have high International Normalized Ratio (INR values on the day of surgery and the procedure may be cancelled. We sought to identify easily available clinical characteristics that may influence the rate of normalization of prothrombin time when warfarin is stopped before surgery or invasive procedures. Methods Clinical data were collected retrospectively from consecutive cases from two cohorts, who stopped warfarin 6 days before surgery. An INR value of 1.6 or higher on the day of surgery or requirement for reversal with vitamin K the day before surgery were criteria for slow return (S to normal INR. Results Of 202 patients, 14 (7% were classified as S. Eight of the S-patients required reversal with vitamin K one day before surgery and in another case surgery was cancelled due to high INR. Baseline INR was the only variable significantly associated with classification as S in stepwise logistic regression analysis (p = 0.003. The odds ratio for being in the normal group was 0.27 (95% confidence interval 0.12–0.62 for each unit baseline INR increased. The positive predictive value of baseline INR with a cut off at > 3.0 was only 15% and for INR > 3.5 it was 33%. Conclusion Baseline INR, but not the size of the maintenance dose, is associated with the rate of normalization of prothrombin time after stopping warfarin, but it has limited utility as predictor in clinical practice. Whenever normal hemostasis is considered crucial for the safety, the INR should be checked again before the invasive procedure.

  20. Effects of sugammadex on activated partial thromboplastin time and prothrombin time in healthy subjects.

    Science.gov (United States)

    De Kam, Pieter-Jan; Grobara, Peter; Prohn, Marita; Höppener, Floris; Kluft, Cornelis; Burggraaf, Jacobus; Langdon, Ronald B; Peeters, Pierre

    2014-03-01

    To assess the impact of sugammadex on activated partial thromboplastin time (APTT) and international normalized ratio for prothrombin time (PT(INR)) in healthy subjects and characterize the concentration-dependency of sugammadex effects on APTT and prothrombin time (PT) in normal human plasma in vitro. Eight healthy subjects (18 - 45 years of age) were administered intravenous doses of 4 mg/kg sugammadex, 16 mg/kg sugammadex, or placebo in a randomized, placebo-controlled, three period cross-over trial. The primary endpoint was area under the curve from 2 to 60 minutes post-dose (AUC2-60min) for APTT and PT(INR). In vitro, the effects of sugammadex on APTT and PT were assessed in pooled normal human citrate plasma. In subjects dosed with 4 and 16 mg/kg sugammadex, geometric mean ratios (treated vs. placebo) for AUC2-60min were 1.085 (95% confidence interval, 0.888 - 1.325) and 1.019 (0.868 - 1.195), respectively, for APTT, and 1.047 (0.904 - 1.213) and 1.096 (0.953 - 1.261), respectively, for PT(INR). At individual timepoints, mean APTT and PT(INR) increased by up to 22% after 16 mg/kg sugammadex compared with placebo. All such increases occurred within 30 minutes post-dose. Sugammadex was generally well tolerated. In the in vitro experiments, addition of sugammadex to plasma resulted in limited, concentration dependent increases in both APTT and PT. At 200 μg/mL (the mean maximum concentration reached therapeutically), the relative increases were 29% and 19%, respectively. Administration of sugammadex is associated with a dose-related, limited and transient prolongation of APTT and PT(INR) that is unlikely to be of clinical relevance.

  1. Des-Gamma-Carboxy Prothrombin (DCP Antagonizes the Effects of Gefitinib on Human Hepatocellular Carcinoma Cells

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    Yu-Sheng Zhang

    2015-01-01

    Full Text Available Background/Aims: Des-gamma-carboxy prothrombin (DCP, an aberrant prothrombin produced by hepatocellular carcinoma (HCC cells, is known as a marker for HCC. Recent studies indicated that high levels of DCP are associated with the malignant potential of HCC. In this study, we aimed to investigate the association of DCP with gefitinib treatment failure in HCC and whether DCP counteracts gefitinib-induced growth inhibition and apoptosis of HCC. Methods: The experiments were performed in HCC cell lines HepG2 and PLC/PRF/5. The effects of gefitinib on HCC in the presence or absence of DCP were evaluated by the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT assay. Apoptotic cells were identified by Annexin V-FITC/PI staining. Western blotting was performed to analyze the expressions of molecules related to the apoptotic caspase-dependent pathway and epidermal growth factor receptor (EGFR pathway. Results: Gefitinib inhibited HCC cell proliferation and induced apoptosis in HCC cells. The effects of gefitinib on HCC cells were antagonized by DCP. In the presence of DCP, HCC cells were resistant to the gefitinib-induced inhibition of proliferation and stimulation of apoptosis. DCP prevented the activation of the apoptotic caspase-dependent pathway induced by gefitinib. These antagonistic effects of DCP also arose from its ability to up-regulate EGFR, c-Met and hepatocyte growth factor (HGF in HCC cells. Conclusion: DCP antagonized gefitinib-induced HCC cell growth inhibition by counteracting apoptosis and up-regulating the EGFR pathway. High levels of DCP might thus lead to low response rates or possibly no response to gefitinib in patients with HCC.

  2. Anti-phosphatidylserine-prothrombin antibodies are associated with outcome in a TIA cohort

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    Michael T Mullen

    2012-09-01

    Full Text Available Background: Antiphospholipid antibodies (aPLs have been associated with thrombosis in the antiphospholipid antibody syndrome (APS and with atherosclerotic vascular events in patients without APS. We examined the significance of aPLs in transient ischemic attack (TIA.Patients/Methods: Patients with TIA <48 hours from symptom onset were prospectively enrolled. Traditional aPLs, including anti-cardiolipin (aCL and β2-glycoprotein-I (β2GPI, and newer aPLs, including anti-phosphatidylserine/prothrombin (aPS/PT, β2GPI Domain 4/5 and β2GPI Domain 1 were measured. Primary outcome was a composite of stroke or death within 90 days or identification of a high-risk stroke mechanism. Secondary outcomes were stroke or death and the presence of clinical/sub-clinical atherosclerosis. Results: Over 4.5 years, 167 patients were enrolled. 41 patients (25% had the composite endpoint. Antibodies were measured in 158 subjects. aPS/PT IgG antibodies were significantly associated with stroke/death (OR 16.3 95% CI 2.3-116.7 p=0.005 and were non-significantly associated with the composite endpoint (OR 4.7 95% CI 0.8-29.2 p=0.10. In multivariate analysis adjusting for ABCD2 risk score, aPS/PT IgG remained associated with stroke/death (OR 15.7 95% CI 2.0-125.6 p=0.009. Other aPLs were not associated with clinical outcome and no association between APLs and atherosclerosis was identifed. Conclusion: In contrast to other aPLs, anti-phosphatidylserine/prothrombin IgG antibodies are independently associated with stroke or death in patients with TIA.

  3. The use of contrast-enhanced ultrasound in the treatment of limb pseudoaneurysm with percutaneous prothrombin injection

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    Zhong CHEN

    2014-03-01

    Full Text Available Objective To explore the safety and effectiveness of contrast-enhanced ultrasound (CEUS-guided percutaneous injection of prothrombin for the treatment of limb pseudoaneurysm (PSA. Methods Sixteen cases of lower limb pseudoaneurysms were treated with contrast-enhanced ultrasound (CEUS-guided percutaneous injection of prothrombin, and the size of the pseudoaneurysm, width and length of the pseudoaneurysm neck, times for compression, duration of each compression, and dose of prothrombin injection were recorded. Results In 16 pseudoaneurysm patients, there were 20 pseudoaneurysm sacs in total. One pseudoaneurysm sac was present in 13 patients, 2 sacs were present in 2, and there were 3 sacs in one patient. The mean maximum diameter of the sacs was 41.1±11.9mm. The mean width and length of these pseudoaneurysm necks were 1.9±0.5mm and 4.4±2.3mm respectively. During the process of treatment, the mean duration for compression was 6.0±2.0min, and the mean number of times of compression was 1.2±0.4. The mean dose of prothrombin injection was 411.5±118.8U. The cure rate with one-injection was 100% (16/16, and the recurrence rate was 0%. No complications was observed. Conclusions CEUS-guided percutaneous injection of prothrombin for the treatment of lower limb pseudoaneurysms is safe and effective. The size of the pseudoaneurysm sac and length of the pseudoaneurysm neck are important factors that may affect the result of the treatment. Timely recompression is an important step for increasing the cure rate. DOI: 10.11855/j.issn.0577-7402.2014.01.09

  4. Blood Coagulation Induced by Iranian Saw-Scaled Viper (Echis Carinatus Venom: Identification, Purification and Characterization of a Prothrombin Activator

    Directory of Open Access Journals (Sweden)

    Mahdi Babaie

    2013-11-01

    Full Text Available   Objective(s: Echis carinatus is one of the venomous snakes in Iran. The venom of Iranian Echis carinatus is a rich source of protein with various factors affecting the plasma protein and blood coagulation factor. Some of these proteins exhibit types of enzymatic activities. However, other items are proteins with no enzymatic activity.   Materials and Methods: In order to study the mechanism and effect of the venom on human plasma proteins, the present study has evaluated the effect of crude venom and all fractions. A procoagulant factor (prothrombin activator was isolated from the venom of the Iranian snake Echis carinatus with a combination of gel filtration (Sephadex G-75, ion-exchange chromatography (DEAE- Sepharose and reverse phase HPLC. Furthermore, proteolytic activity of the crude venom and all fractions on blood coagulation factors such as prothrombin time (PT was studied. Results: In the present study, the PT test was reduced from 13.4 s to 8.6 s when human plasma was treated with crude venom (concentraion of venom was 1 mg/ml. The purified procoagulant factor revealed a single protein band in SDS polyacrylamide electrophoresis under reducing conditions and its molecular weight was estimated at about 65 kDa. A single-band protein showed fragment patterns similar to those generated by the group A prothrombin activators, which convert prothrombin into meizothrombin independent of the prothrombinase complex. Conclusion: This study showed that the fraction which separated from Iranian snake Echis carinatus venom can be a prothrombin activators. It can be concluded that this fraction is a procoagulant factor.

  5. UniProt search blastx result: AK288926 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK288926 J090082E17 Q15772|SPEG_HUMAN Striated muscle preferentially expressed prot...ein kinase (EC 2.7.11.1) (Aortic preferentially expressed protein 1) (APEG-1) - Homo sapiens (Human) 0 ...

  6. Primary thrombophilia in Mexico. II. Factor V G1691A (Leiden), prothrombin G20210A, and methylenetetrahydrofolate reductase C677T polymorphism in thrombophilic Mexican mestizos.

    Science.gov (United States)

    Ruiz-Argüelles, G J; Garcés-Eisele, J; Reyes-Núñez, V; Ramírez-Cisneros, F J

    2001-01-01

    We have shown that in Mexican mestizo patients with clinical features of primary thrombophilia, 39% have activated protein C resistance phenotype, 5% protein C deficiency, and 2% protein S deficiency. In the present study, in a group of 37 thrombophilic Mexicans and 50 normal controls, we assessed the factor V G1691A (Leiden), the prothrombin G20210A, and the methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphisms. Four patients were found to be heterozygous for factor V Leiden, 5 heterozygous for the prothrombin 20210, 16 heterozygous, and 6 homozygous for the MTHFR 677. There were four individuals with co-segregation of alleles: two heterozygotes for the factor V Leiden/prothrombin 20210, one heterozygote for prothrombin 20210/MTHFR 677, and one heterozygote for prothrombin 20210/homozygote for MTHFR 677. For factor V Leiden, prothrombin 20210, and MTHFR 677 mutations, the allele frequencies were respectively 1% (+/-0.2%, alpha = 0.05), 7.66 (P mestizo thrombophilic patients, the low prevalence of the factor V Leiden mutation (10.8%) and the high prevalence of the prothrombin 20210 mutation (13.5%) contrast with those identified in Caucasian thrombophilic patients (21% and 6%, respectively; P mestizo patients.

  7. Risk of venous thromboembolism and myocardial infarction associated with factor V Leiden and prothrombin mutations and blood type.

    Science.gov (United States)

    Sode, Birgitte F; Allin, Kristine H; Dahl, Morten; Gyntelberg, Finn; Nordestgaard, Børge G

    2013-03-19

    ABO blood type locus has been reported to be an important genetic determinant of venous and arterial thrombosis in genome-wide association studies. We tested the hypothesis that ABO blood type alone and in combination with mutations in factor V Leiden R506Q and prothrombin G20210A is associated with the risk of venous thromboembolism and myocardial infarction in the general population. We used data from 2 Danish studies that followed members of the general public from 1977 through 2010. We obtained the genotype of 66 001 white participants for ABO blood type, factor V Leiden R506Q and prothrombin G20210A. We calculated hazard ratios (HRs) and population attributable risk. Our main outcome measures were venous thromboembolism and myocardial infarction. The multivariable adjusted HR for venous thromboembolism was 1.4 (95% confidence interval [CI] 1.3-1.5) for non-O blood type (v. O blood type). For the factor V Leiden R506Q mutation, the adjusted HR was 2.2 (95% CI 2.0-2.5) for heterozygous participants and 7.0 (95%CI 4.8-10) for homozygous participants (v. participants without the mutation). For prothrombin G20210A, the adjusted HR was 1.5 (95%CI 1.2-1.9) for heterozygous participants and 11 (95% CI 2.8-44) for homozygous participants (v. participants without the mutation). When we combined ABO blood type and factor V Leiden R506Q or prothrombin G20210A genotype, there was a stepwise increase in the risk of venous thromboembolism (trend, pfactor V Leiden R506Q and 1% for prothrombin G20210A. Multivariable adjusted HRs for myocardial infarction by genotypes did not differ from 1.0. ABO blood type had an additive effect on the risk of venous thromboembolism when combined with factor V Leiden R506Q and prothrombin G20210A mutations; blood type was the most important risk factor for venous thromboembolism in the general population.

  8. Prothrombin and risk of venous thromboembolism, ischemic heart disease and ischemic cerebrovascular disease in the general population

    DEFF Research Database (Denmark)

    Weischer, Maren; Juul, Klaus; Zacho, Jeppe

    2010-01-01

    OBJECTIVE: We tested the hypotheses that Prothrombin G20210A heterozygosity associate with increased risk of venous thromboembolism (VTE), ischemic heart disease (IHD), and ischemic cerebrovascular disease (ICVD) in the general population and re-tested risk of IHD and ICVD in two case......-control studies. METHODS: 9231 individuals from the Danish general population were followed for VTE (VTE=DVT+PE), deep venous thrombosis (DVT), pulmonary embolism (PE), IHD, myocardial infarction (MI), ICVD, and ischemic stroke (IS) for a median of 24 years. Case-control studies included 2461 IHD cases and 867.......0(1.0-3.8) for MI, 1.4(0.7-3.1) for ICVD, and 2.1(0.8-5.4) for IS. CONCLUSION: Prothrombin G20210A heterozygosity alone and in combination with Factor V Leiden R506Q heterozygosity predicts 1.5 and 6.0 fold risk of IHD compared to non-carriers....

  9. [Recurrent vascular access trombosis associated with the prothrombin mutation G20210A in a adult patient in haemodialysis].

    Science.gov (United States)

    Quintana, L F; Coll, E; Monteagudo, I; Collado, S; López-Pedret, J; Cases, A

    2005-01-01

    Vascular access-related complications are a frequent cause of morbidity in haemodialysis patients and generate high costs. We present the case of an adult patient with end-stage renal disease and recurrent vascular access thrombosis associated with the prothrombin mutation G20210A and renal graft intolerance. The clinical expression of this heterozygous gene mutation may have been favoured by inflammatory state, frequent in dialysis patients. In this patient, the inflammatory response associated with the renal graft intolerance would have favored the development of recurrent vascular access thrombosis in a adult heterozygous for prothrombin mutation G20210A. In the case of early dysfunction of haemodialysis vascular access and after ruling out technical problems, it is convenient to carry out a screening for thrombophilia.

  10. Risk of venous thromboembolism and myocardial infarction associated with factor V Leiden and prothrombin mutations and blood type

    DEFF Research Database (Denmark)

    Sode, Birgitte F; Allin, Kristine H; Dahl, Morten

    2013-01-01

    ABO blood type locus has been reported to be an important genetic determinant of venous and arterial thrombosis in genome-wide association studies. We tested the hypothesis that ABO blood type alone and in combination with mutations in factor V Leiden R506Q and prothrombin G20210A is associated...... with the risk of venous thromboembolism and myocardial infarction in the general population....

  11. Outcome of Patients with Venous Thromboembolism and Factor V Leiden or Prothrombin 20210 Carrier Mutations During the Course of Anticoagulation.

    Science.gov (United States)

    Tzoran, Inna; Papadakis, Manolis; Brenner, Benjamin; Fidalgo, Ángeles; Rivas, Agustina; Wells, Philip S; Gavín, Olga; Adarraga, María Dolores; Moustafa, Farès; Monreal, Manuel

    2017-04-01

    Individuals with factor V Leiden or prothrombin G20210A mutations are at a higher risk to develop venous thromboembolism. However, the influence of these polymorphisms on patient outcome during anticoagulant therapy has not been consistently explored. We used the Registro Informatizado de Enfermedad TromboEmbólica database to compare rates of venous thromboembolism recurrence and bleeding events occurring during the anticoagulation course in factor V Leiden carriers, prothrombin mutation carriers, and noncarriers. Between March 2001 and December 2015, 10,139 patients underwent thrombophilia testing. Of these, 1384 were factor V Leiden carriers, 1115 were prothrombin mutation carriers, and 7640 were noncarriers. During the anticoagulation course, 160 patients developed recurrent deep vein thrombosis and 94 patients developed pulmonary embolism (16 died); 154 patients had major bleeding (10 died), and 291 patients had nonmajor bleeding. On multivariable analysis, factor V Leiden carriers had a similar rate of venous thromboembolism recurrence (adjusted hazard ratio [HR], 1.16; 95% confidence interval [CI], 0.82-1.64), half the rate of major bleeding (adjusted HR, 0.50; 95% CI, 0.25-0.99) and a nonsignificantly lower rate of nonmajor bleeding (adjusted HR, 0.66; 95% CI, 0.43-1.01) than noncarriers. Prothrombin mutation carriers and noncarriers had a comparable rate of venous thromboembolism recurrence (adjusted HR, 1.00; 95% CI, 0.68-1.48), major bleeding (adjusted HR, 0.75; 95% CI, 0.42-1.34), and nonmajor bleeding events (adjusted HR, 1.10; 95% CI, 0.77-1.57). During the anticoagulation course, factor V Leiden carriers had a similar risk for venous thromboembolism recurrence and half the risk for major bleeding compared with noncarriers. This finding may contribute to decision-making regarding anticoagulation duration in selected factor V Leiden carriers with venous thromboembolism. Copyright © 2017. Published by Elsevier Inc.

  12. Effects of Tramadol Coadministration on Prothrombin Time-International Normalized Ratio in Patients Receiving Warfarin.

    Science.gov (United States)

    Hosono, Tomomi; Kondo, Aiko; Kambayashi, Yasuyuki; Homma, Masato

    2017-01-01

     Several case studies have reported a possible drug interaction between warfarin and tramadol where tramadol coadministration enhanced the antithrombotic effects of warfarin. To assess this drug interaction, changes in prothrombin time-international normalized ratio (PT-INR) before and after tramadol coadministration were investigated in patients receiving warfarin. For this study, we examined 54 patients (male/female: 22/32, 68.4±12.7 years) who were being treated with warfarin for deep vein thrombosis, atrial fibrillation, arteriosclerosis obliterans, congestive heart failure, and other vascular diseases. Significant increases in PT-INR were observed 9.5 (1-118) d after coadministration of tramadol (1.81±0.56 vs. 2.47±1.10, ptramadol were compared with other groups of patients to find drug interaction risk factors. Logistic regression analysis revealed that lower levels of albumin (3.5 g/dL or less) [odds ratio (OR) 22.1; 95%CI 2.9-169.9]; lower eGFR (50 mL/min or less) (OR 7.7; 95%CI 1.4-42.0); and PT-INR before tramadol coadministration (OR 38.2; 95%CI 3.7-397.6) were characteristic of the PT-INR increased group. These results suggest that tramadol coadministration enhanced the antithrombotic effects of warfarin in patients with higher PT-INR, lower albumin levels and decreased renal function as the risk factors for this drug interaction.

  13. [False positive serum des-gamma-carboxy prothrombin after resection of hepatocellular carcinoma].

    Science.gov (United States)

    Hiramatsu, Kumiko; Tanaka, Yasuhito; Takagi, Kazumi; Iida, Takayasu; Takasaka, Yoshimitsu; Mizokami, Masashi

    2007-04-01

    Measurements of serum concentrations of des-gamma-carboxy-prothrombin (PIVKA-II) are widely used for diagnosing hepatocellular carcinoma (HCC). Recently, when we evaluated the correlation of PIVKA-II between two commercially available PIVKA-II immunoassay kits (Lumipulse f vs. Picolumi) to introduce it in our hospital, false high values of PIVKA-II were observed in Lumipulse assay. Four(4%) of 100 serum samples showed false high values, and all of them were obtained from patients less than 2 month after curative resection of HCC. Examining additional 7 patients with HCC resection, serum samples from the 5 patients had the same trend. To elucidate the non-specific reaction by Lumipulse assay which utilized alkaline phosphatase (ALP) enzymatic reaction, inhibition assays by various absorbents such as inactive ALP and IgM antibodies were performed. Excess of inactive ALP reduced the high values of PIVKA-II. Note that anti-bleeding sheets (fibrinogen combined drug), which included bovine thrombin, were directly attached on liver of all patients with HCC resection in this study. As the sheets also contaminate ALP and probably produce IgM antibodies to ALP, the IgM may cross-react with anti-PIVKA-II antibodies directly. Taken together, it was suggested that produced antibodies against ALP derived from anti-bleeding sheets led false high values of PIVKA-II in the patients with HCC resection.

  14. Management of the Bleeding Patient Receiving New Oral Anticoagulants: A Role for Prothrombin Complex Concentrates

    Directory of Open Access Journals (Sweden)

    Lisa M. Baumann Kreuziger

    2014-01-01

    Full Text Available Ease of dosing and simplicity of monitoring make new oral anticoagulants an attractive therapy in a growing range of clinical conditions. However, newer oral anticoagulants interact with the coagulation cascade in different ways than traditional warfarin therapy. Replacement of clotting factors will not reverse the effects of dabigatran, rivaroxaban, or apixaban. Currently, antidotes for these drugs are not widely available. Fortunately, withholding the anticoagulant and dialysis are freqnently effective treatments, particularly with rivaroxaban and dabigatran. Emergent bleeding, however, requires utilization of Prothrombin Complex Concentrates (PCCs. PCCs, in addition to recombinant factor VIIa, are used to activate the clotting system to reverse the effects of the new oral anticoagulants. In cases of refractory or emergent bleeding, the recommended factor concentrate in our protocols differs between the new oral anticoagulants. In patients taking dabigatran, we administer an activated PCC (aPCC [FELBA] due to reported benefit in human in vitro studies. Based on human clinical trial evidence, the 4-factor PCC (Kcentra is suggested for patients with refractory rivaroxaban- or apixaban-associated hemorrhage. If bleeding continues, recombinant factor VIIa may be employed. With all of these new procoagulant agents, the risk of thrombosis associated with administration of factor concentrates must be weighed against the relative risk of hemorrhage.

  15. Levels of acarboxy prothrombin (PIVKA-II) and coagulation factors in warfarin-treated patients.

    Science.gov (United States)

    Umeki, S; Umeki, Y

    1990-04-01

    PIVKA-II (protein induced by vitamin K absence or antagonists-II) was determined and compared with other coagulation factors in normal subjects and patients treated with the anticoagulant warfarin. In 18 (60%) of 30 patients treated with warfarin, PIVKA-II values were 1 microgram/ml or more, although they were less than 1 microgram/ml in all 39 normal subjects (100%). In patients treated with warfarin, values of prothrombin time and partial thromboplastin time were significantly higher than those in normal subjects. However, values of hepaplastintest (normotest) and thrombotest in the patients were greatly lower than those in normal subjects. There were no significant differences between bleeding time or plasma fibrinogen values in the patients and normal subjects. The values of PIVKA-II were inversely correlated (P less than 0.01) with those of hepaplastintest and thrombotest. The measurement of PIVKA-II in the plasma should be useful in detecting vitamin K-deficient status among haemorrhagic disorders.

  16. A novel prothrombin time assay for assessing the anticoagulant activity of oral factor Xa inhibitors.

    Science.gov (United States)

    Barrett, Yu Chen; Wang, Zhaoqing; Knabb, Robert M

    2013-09-01

    Conventional prothrombin time (PT) assays have limited sensitivity and dynamic range in monitoring the anticoagulant activity of direct factor Xa inhibitors. Hence, new assays are needed. We modified a PT assay by adding calcium chloride (CaCl2) to the thromboplastin reagent to increase assay dynamic range and improve sensitivity. Effects of calcium and sodium ion concentrations, and sample handling, were evaluated to optimize assay performance. Increasing concentrations of calcium ions produced progressive increases in PT across the factor Xa inhibitor concentrations of 0 to 2500 nmol/L for razaxaban and apixaban. The greatest effect was seen when the thromboplastin reagent was diluted 1:2.25 with 100 mmol/L CaCl2 (thus selected for routine use). The optimized assay showed an interassay precision of 1.5 to 9.3 percentage coefficient of variation (%CV) for razaxaban and 3.1 to 4.6 %CV for apixaban. We conclude that the modified PT assay is likely to be suitable as a pharmacodynamic marker for activity at therapeutic concentrations of factor Xa inhibitors.

  17. Antibodies to Phosphatidylserine/Prothrombin Complex in Antiphospholipid Syndrome: Analytical and Clinical Perspectives.

    Science.gov (United States)

    Peterson, Lisa K; Willis, Rohan; Harris, E Nigel; Branch, Ware D; Tebo, Anne E

    2016-01-01

    Antiphospholipid syndrome (APS) is an autoimmune disorder characterized by thrombosis and/or pregnancy-related morbidity accompanied by persistently positive antiphospholipid antibodies (aPL). Current laboratory criteria for APS classification recommend testing for lupus anticoagulant as well as IgG and IgM anticardiolipin, and beta-2 glycoprotein I (anti-β2GPI) antibodies. However, there appears to be a subset of patients with classical APS manifestations who test negative for the recommended criteria aPL tests. While acknowledging that such patients may have clinical features that are not of an autoimmune etiology, experts also speculate that these "seronegative" patients may test negative for relevant autoantibodies as a result of a lack of harmonization and/or standardization. Alternatively, they may have aPL that target other antigens involved in the pathogenesis of APS. In the latter, autoantibodies that recognize a phosphatidylserine/prothrombin (PS/PT) complex have been reported to be associated with APS and may have diagnostic relevance. This review highlights analytical and clinical attributes associated with PS/PT antibodies, taking into consideration the performance characteristics of criteria aPL tests in APS with specific recommendations for harmonization and standardization efforts.

  18. Diagnostic Performance of Des-γ-carboxy Prothrombin for Hepatocellular Carcinoma: A Meta-Analysis

    Directory of Open Access Journals (Sweden)

    Rong Zhu

    2014-01-01

    Full Text Available Background. There have been many reports on des-γ-carboxy prothrombin (DCP as a promising serum marker in the diagnosis of hepatocellular carcinoma (HCC; however, the results are inconsistent and even conflicting. Methods. This meta-analysis was performed to investigate the performance of DCP in the diagnosis of HCC. Following a systematic review of relevant studies, Meta-DiSc 1.4 software was used to extract data and to calculate the overall sensitivity, specificity, positive likelihood ratio (PLR, negative likelihood ratio (NLR, and diagnostic odds ratio (DOR. Data are presented as forest plots and summary receiver operating characteristic curve (SROC analysis was used to summarize the overall test performance. Results. Twelve studies were included in our meta-analysis. The overall sensitivity, specificity, PLR, and NLR of DCP for the detection of HCC in the studies included were 71% (95%CI: 68%–73%, 84% (95%CI: 83%–86%, 6.48 (95%CI: 4.22–9.93, and 0.33 (95%CI: 0.25–0.43, respectively. The area under the SROC curve was 0.8930 and the Q index was 0.8238. Significant heterogeneity was found. Conclusion. This meta-analysis indicated that DCP had moderate diagnostic accuracy in HCC. Further studies with rigorous design, large sample size, and mmultiregional cooperation are needed in the future.

  19. Risk of recurrent venous thrombosis in homozygous carriers and double heterozygous carriers of factor V Leiden and prothrombin G20210A.

    Science.gov (United States)

    Lijfering, Willem M; Middeldorp, Saskia; Veeger, Nic J G M; Hamulyák, Karly; Prins, Martin H; Büller, Harry R; van der Meer, Jan

    2010-04-20

    Homozygous or double heterozygous factor V Leiden and/or prothrombin G20210A is a rare inherited thrombophilic trait. Whether individuals with this genetic background have an increased risk of recurrent venous thrombosis is uncertain. A case-control design within a large cohort of families with thrombophilia was chosen to calculate the risk of recurrent venous thrombosis in individuals with homozygosity or double heterozygosity of factor V Leiden and/or prothrombin G20210A. Cases were individuals with recurrent venous thrombosis, and controls were those with only 1 venous thrombosis. The cohort consisted of 788 individuals with venous thrombosis; 357 had factor V Leiden, 137 had prothrombin G20210A, 27 had factor V Leiden and/or prothrombin G20210A homozygosity, and 49 had double heterozygosity for both mutations. We identified 325 cases with recurrent venous thrombosis and 463 controls with only 1 venous thrombosis. Compared with noncarriers, crude odds ratio for recurrence was 1.2 (95% confidence interval, 0.9 to 1.6) for heterozygous carriers of factor V Leiden, 0.7 (95% confidence interval, 0.4 to 1.2) for prothrombin G20210A, 1.2 (95% confidence interval, 0.5 to 2.6) for homozygous carriers of factor V Leiden and/or prothrombin G20210A, and 1.0 (95% confidence interval, 0.6 to 1.9) for double heterozygotes of both mutations. Adjustments for age, sex, family status, first event type, and concomitance of natural anticoagulant deficiencies did not alter the risk estimates. In this study, individuals with homozygous factor V Leiden and/or homozygous prothrombin G20210A or double heterozygous carriers of factor V Leiden and prothrombin G20210A did not have a high risk of recurrent venous thrombosis.

  20. The risk of early mortality of polytrauma patients associated to ISS, NISS, APACHE II values and prothrombin time.

    Science.gov (United States)

    Mica, Ladislav; Rufibach, Kaspar; Keel, Marius; Trentz, Otmar

    2013-01-01

    The early hemodynamic normalization of polytrauma patients may lead to better survival outcomes. The aim of this study was to assess the diagnostic quality of trauma and physiological scores from widely used scoring systems in polytrauma patients. In total, 770 patients with ISS > 16 who were admitted to a trauma center within the first 24 hours after injury were included in this retrospective study. The patients were subdivided into three groups: those who died on the day of admission, those who died within the first three days, and those who survived for longer than three days. ISS, NISS, APACHE II score, and prothrombin time were recorded at admission. The descriptive statistics for early death in polytrauma patients who died on the day of admission, 1-3 days after admission, and > 3 days after admission were: ISS of 41.0, 34.0, and 29.0, respectively; NISS of 50.0, 50.0, and 41.0, respectively; APACHE II score of 30.0, 25.0, and 15.0, respectively; and prothrombin time of 37.0%, 56.0%, and 84%, respectively. These data indicate that prothrombin time (AUC: 0.89) and APACHE II (AUC: 0.88) have the greatest prognostic utility for early death. The estimated densities of the scores may suggest a direction for resuscitative procedures in polytrauma patients. "Retrospektive Analysen in der Chirurgischen Intensivmedizin"StV01-2008.

  1. Clinical characteristics of patients with factor V Leiden or prothrombin G20210A and a first episode of venous thromboembolism. Findings from the RIETE Registry.

    Science.gov (United States)

    Gadelha, Telma; Roldán, Vanessa; Lecumberri, Ramón; Trujillo-Santos, Javier; del Campo, Raquel; Poggio, Renzo; Monreal, Manuel

    2010-10-01

    The clinical characteristics of patients with factor V Leiden or prothrombin G20210A presenting with a first episode of venous thromboembolism (VTE) have not been thoroughly studied. RIETE is an ongoing registry of consecutive patients with acute VTE. We compared the clinical characteristics of patients with factor V Leiden, prothrombin G20210A, or no thrombophilia, at presentation with a first episode of VTE. As of May 2009, 22428 patients had been enrolled with a first episode of VTE. Of these, 345 had factor V Leiden, 261 had prothrombin G20210A, and 2399 tested negative. Sixty-two percent of the VTE episodes in women with factor V Leiden or prothrombin G20210A (40% in men) were associated with an acquired risk factor. Among women, pregnancy or contraceptive use accounted for 63% and 67% of such risk factors. Patients with factor V Leiden presented with pulmonary embolism (PE) less likely than those with prothrombin G20210A (31% vs. 51%; pFactor V Leiden presented with hypoxaemia (Sat O(2) levelsfactor V Leiden or prothrombin G20210A were associated with an acquired risk factor (mostly pregnancy or contraceptive use). Only 4.5% of patients with factor V Leiden presenting with acute PE had hypoxaemia. Copyright © 2010 Elsevier Ltd. All rights reserved.

  2. Prevalence of factor V G1691A (Leiden) and prothrombin G20210A polymorphisms among apparently healthy Jordanians.

    Science.gov (United States)

    Nusier, Mohamad K; Radaideh, Abdelrahman M; Ababneh, Nida'a A; Qaqish, Bara'ah M; Alzoubi, Renad; Khader, Yousef; Mersa, Janet Y; Irshaid, Nidal M; El-Khateeb, Mohammed

    2007-10-01

    Factor V Leiden and prothrombin G20210A are related genetic risk factors for venous thromboembolism (VTE). Analysis for both mutations is increasingly being performed on patients exhibiting hypercoagulability. The objective of this study was to determine the prevalence of factor V Leiden (FVL), prothrombin-G20210A (PT-G20210A) polymorphisms and their coexistence among apparently healthy Jordanians. One thousand apparently healthy individuals from representative regions of Jordan with no previous history of VTE participated in this study. The mean age of participants was 28.5+/-6.4 years (age range 18-45 years). Two hundred and eighteen subjects were APC resistant with an APC-R mean of 85.52+/-15.35 seconds; the non-resistant subjects had an APC-R mean of 159.90+/-26.96 seconds. A multiplex polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the simultaneous detection of FVL and prothrombin G20210A was used to analyze the 218 DNA samples that were APC-R resistant. Both mutations generate HindIII RFLPs and the prothrombin amplicon contains an invariant HindIII recognition sites. The multiplex PCR-RFLP of Factor V for those 218-samples was: 41 wild-type, 169 heterozygous mutant, and eight homozygous mutant individuals. For prothrombin G20210A, the multiplex PCR-RFLP identified 215 wild-type and three heterozygous mutant individuals. Factor V positive individuals (n=50) had a mean F-V activity of 78.04%+/-25.81. F-V activity among wild type (n=41), F-V Leiden heterozygous (n=169) and F-V Leiden homozygous (n=8) were 92.93%+/-16.17, 87.02%+/-15.21 and 96.14%+/-12.32, respectively. Factor II positive subjects (n=47) had a mean factor II activity of 127.96%+/-21.37. F-II activity among carriers (heterozygous, n=3) and non-carriers (normal, n=215) of PT-G20210A mutation were 107.67%+/-9.29 and 105.00%+/-17.79, respectively. The prevalence of FVL was 21.8% and there is a little likelihood of the co-inheritance of the FVL and PT-G20210A among

  3. SwissProt search result: AK061101 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_BOVIN 4e-18 ... ...AK061101 006-207-A12 (P00735) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  4. SwissProt search result: AK112005 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_RAT 1e-13 ... ...AK112005 006-202-F10 (P18292) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  5. SwissProt search result: AK061101 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_RAT 2e-16 ... ...AK061101 006-207-A12 (P18292) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  6. SwissProt search result: AK109059 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_BOVIN 1e-16 ... ...AK109059 002-154-F02 (P00735) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  7. SwissProt search result: AK061101 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_HUMAN 2e-17 ... ...AK061101 006-207-A12 (P00734) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  8. SwissProt search result: AK112119 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_HUMAN 7e-17 ... ...AK112119 006-303-H09 (P00734) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  9. SwissProt search result: AK109059 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_MOUSE 6e-17 ... ...AK109059 002-154-F02 (P19221) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  10. SwissProt search result: AK061101 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_PONPY 8e-18 ... ...AK061101 006-207-A12 (Q5R537) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  11. SwissProt search result: AK109059 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_PONPY 3e-17 ... ...AK109059 002-154-F02 (Q5R537) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  12. SwissProt search result: AK112005 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_BOVIN 8e-16 ... ...AK112005 006-202-F10 (P00735) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  13. SwissProt search result: AK061101 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_MOUSE 9e-17 ... ...AK061101 006-207-A12 (P19221) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  14. SwissProt search result: AK109059 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_HUMAN 9e-18 ... ...AK109059 002-154-F02 (P00734) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  15. SwissProt search result: AK112005 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_PONPY 1e-15 ... ...AK112005 006-202-F10 (Q5R537) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  16. SwissProt search result: AK112119 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_MOUSE 7e-15 ... ...AK112119 006-303-H09 (P19221) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  17. SwissProt search result: AK112005 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_HUMAN 2e-16 ... ...AK112005 006-202-F10 (P00734) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  18. SwissProt search result: AK112005 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_MOUSE 2e-14 ... ...AK112005 006-202-F10 (P19221) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  19. SwissProt search result: AK109059 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_RAT 2e-16 ... ...AK109059 002-154-F02 (P18292) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  20. SwissProt search result: AK112119 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_PONPY 5e-16 ... ...AK112119 006-303-H09 (Q5R537) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  1. SwissProt search result: AK112119 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_BOVIN 3e-16 ... ...AK112119 006-303-H09 (P00735) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  2. SwissProt search result: AK112119 [KOME

    Lifescience Database Archive (English)

    Full Text Available ion peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain] THRB_RAT 3e-14 ... ...AK112119 006-303-H09 (P18292) Prothrombin precursor (EC 3.4.21.5) (Coagulation factor II) [Contains: Activat

  3. Genetic variations and diseases in UniProtKB/Swiss-Prot: the ins and outs of expert manual curation.

    Science.gov (United States)

    Famiglietti, Maria Livia; Estreicher, Anne; Gos, Arnaud; Bolleman, Jerven; Géhant, Sébastien; Breuza, Lionel; Bridge, Alan; Poux, Sylvain; Redaschi, Nicole; Bougueleret, Lydie; Xenarios, Ioannis

    2014-08-01

    During the last few years, next-generation sequencing (NGS) technologies have accelerated the detection of genetic variants resulting in the rapid discovery of new disease-associated genes. However, the wealth of variation data made available by NGS alone is not sufficient to understand the mechanisms underlying disease pathogenesis and manifestation. Multidisciplinary approaches combining sequence and clinical data with prior biological knowledge are needed to unravel the role of genetic variants in human health and disease. In this context, it is crucial that these data are linked, organized, and made readily available through reliable online resources. The Swiss-Prot section of the Universal Protein Knowledgebase (UniProtKB/Swiss-Prot) provides the scientific community with a collection of information on protein functions, interactions, biological pathways, as well as human genetic diseases and variants, all manually reviewed by experts. In this article, we present an overview of the information content of UniProtKB/Swiss-Prot to show how this knowledgebase can support researchers in the elucidation of the mechanisms leading from a molecular defect to a disease phenotype.

  4. Diagnostic value of antibodies to phosphatidylserine/prothrombin complex for antiphospholipid syndrome in Chinese patients.

    Science.gov (United States)

    Zhu, Lei; Li, Chun; Liu, Na; Yang, Xin; Jia, R L; Mu, Rong; Su, Yin; Li, Z G

    2017-02-01

    To evaluate the diagnosis value of antibodies to phosphatidylserine/prothrombin complex (aPS/PT) in patients with antiphospholipid syndrome (APS) and to determine the clinical features of APS patients with avidity of aPS/PT. Serum samples were collected from 108 APS patients. Sixty patients with pregnancy morbidity, 37 patients with thrombosis without a history of autoimmune diseases, and 89 healthy blood donors were included as the control group. The enzyme-linked immunosorbent assay (ELISA) test was performed to detect the concentration of aPS/PT, including IgG/M, IgG, and IgM forms, in the same serum sample. The chi-square (χ2) test was used to examine the difference of frequencies of antibodies in APS patients and patients with other diseases. Spearman correlation analysis was performed to investigate the relationship between aPS/PT and other clinical/laboratory parameters. aPS/PT was detectable in 68 (63.0%) of the 108 APS patients, 12 (13.2%) of the 91 disease control patients and 1 (1.1%) of the healthy controls. It was strongly correlated with the activity of lupus anticoagulant (LA) (OR 15.952, 95% CI 7.132-35.678; P < 0.001). The frequency of aPS/PT was 56.9% in anti-cardiolipid antibody (aCL)-negative, 60.5% anti-β2 glycoprotein I antibody (aβ2GPI)-negative, and 50.0% in both aCL and aβ2GPI negative APS patients. The IgG aPS/PT was significantly associated with arterial and venous thrombosis. The aPS/PT antibody could play an important role in the diagnosis of APS, especially in patients with negative aCL and aβ2GPI. It was positively related to thrombotic events in APS.

  5. Anti-Phosphatidylserine/Prothrombin Antibodies Are Associated with Adverse Pregnancy Outcomes

    Directory of Open Access Journals (Sweden)

    Polona Žigon

    2015-01-01

    Full Text Available Objective. To determine the prevalence and clinical association of anti-phosphatidylserine/prothrombin antibodies (aPS/PT in patients with a history of pregnancy complications relevant to antiphospholipid syndrome (APS. Materials and Methods. Two hundred and eleven patients with a history of (a three or more consecutive miscarriages before 10th week of gestation (WG (n=64, (b death of a morphologically normal fetus beyond 10th WG (n=72, (c premature birth of a morphologically normal neonate before 34th WG due to eclampsia, preeclamsia and placental insufficiency (n=33, and (d less than three unexplained consecutive miscarriages before 10th WG (n=42. Subjects sera were analyzed for lupus anticoagulant (LA, anti-cardiolipin (aCL, anti-β2-glycoprotein I (anti-β2GPI, and aPS/PT antibodies. Results. 41/169 (24.3% of patients were positive for at least one measured aPL. The highest prevalence was found for aPS/PT and aCL (13.0% and 12.4%, resp. followed by LA (7.7% and anti-β2GPI (7.1%. 11/169 with APS-related obstetric manifestations had only aPS/PT. 17.8% of patients were positive for LA or aCL and/or anti-β2GPI; however when adding the aPS/PT results, an additional 7% of patients could be evaluated for APS. Conclusion. aPS/PT are associated with recurrent early or late abortions and with premature delivery irrespective of other aPL.

  6. Restoration of Normal Prothrombin Time/International Normalized Ratio With Fresh Frozen Plasma in Hypocoagulable Patients.

    Science.gov (United States)

    Only, Arthur J; DeChristopher, Phillip J; Iqal, Omer; Fareed, Jawed

    2016-01-01

    Fresh frozen plasma (FFP) is an effective reversal agent for hypocoagulable patients. Its proven efficacy continues to prompt its usage as both a prophylactic and a therapeutic therapy. Although published guidelines encouraging the appropriate administration of FFP exist, overutilization continues. The purpose of these ex vivo studies was to determine the effects of succeeding volumes of FFP supplementation on hypocoagulable plasma prothrombin time/international normalized ratio (PT/INR). By analyzing the decline in PT/INR with varying volumes of FFP, a minimal required volume of FFP could be identified representing the optimal volume to administer while still providing therapeutic effect. A total of 497 plasma samples were screened for elevated PT/INR values and 50 samples were selected for inclusion in this experiment. The initial PTs/INRs ranged from 12.5 to 43.4 seconds/1.42 to 4.91. Subsequent declines in PT/INR values were analyzed following addition of 50, 100, and 150 µL of FFP to a fixed volume of 250 µL of plasma (26.4 ± 5.318 seconds/2.99 ± 0.603, 13.3 ± 1.077 seconds/1.51 ± 0.122, 11.2 ± 0.712 seconds/1.27 ± 0.081, and 10.3 ± 0.533 seconds/1.16 ± 0.06, respectively). A nonlinear relationship between decline in INR values and percentage of FFP supplementation was demonstrated. The greatest effect on INR was obtained after supplementation with 50 µL (49%). Doubling and tripling the volume of FFP lead to significantly lower declines in INR (16% and 8%, respectively). Analysis of variance indicated a statistical significance with subsequent volume supplementation of FFP, but marginal clinical benefits exist between the PTs/INRs obtainable with increased FFP volume administration.

  7. Meta-analysis of factor V Leiden and prothrombin G20210A polymorphism in migraine.

    Science.gov (United States)

    Lippi, Giuseppe; Mattiuzzi, Camilla; Cervellin, Gianfranco

    2015-01-01

    Migraine is a frequent and disabling condition, which exhibits a substantial genetic background and is frequently associated with abnormalities of primary and secondary hemostasis. We performed a systematic literature search and a meta-analysis of available data about the potential associations between migraine and factor V (FV) Leiden or prothrombin (FII) G20210A gene polymorphism. The final number of studies included was 15 (all cross-sectional) about migraine and FV Leiden, and 12 (all cross-sectional) about migraine and FII G20210A polymorphism, with broad inter-study heterogeneity (I², 82 and 85%). The cumulative prevalence of the FV 1691A allele was found to be similar between cases (n = 1450; 4.9%) and controls (n = 3468; 4.7%; P = 0.74). The cumulative prevalence of the FII 20210A allele was also found to be similar between cases (n = 1226; 4.2%) and controls (n = 3144; 4.5%; P = 0.59). Nevertheless, sub-analysis of studies in adults and children revealed that both polymorphisms were not associated with migraine in adults, but FV Leiden and the FII 20210A allele were approximately two-fold more prevalent in children with migraine than in those without. In conclusion, despite migraine exhibits a clear neurovascular origin and is frequently associated with thrombotic disorders, isolate thrombophilic mutations seem to play a negligible pathogenetic role in this condition in adults, whereas the increased prevalence of FV Leiden and the FII 20210A allele in children with migraine deserves further scrutiny.

  8. Des-gamma-carboxy prothrombin as an important prognostic indicator in patients with small hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Kenichi Hakamada; Norihisa Kimura; Takuya Miura; Hajime Morohashi; Keinosuke Ishido; Masaki Nara; Yoshikazu Toyoki; Shunji Narumi; Mutsuo Sasaki

    2008-01-01

    AIM:To clarify the effect of a high des-gamma-carboxy prothrombin (DCP) level on the invasiveness and prognosis of small hepatocellular carcinoma.METHODS:Among 142 consecutive patients with known DCP levels,who underwent hepatectomy because of hepatocellular carcinoma,85 patients met the criteria for small hepatocellular carcinoma,i.e.one≤5 cm sized single tumor or no more than three≤3 cm sized tumors.RESULTS:The overall survival rate of the 142 patients was 92.1% for 1 year,69.6% for 3 years,and 56.9% for 5 years.Multivariate analysis showed that microscopic vascular invasion (P = 0.03) and serum DCP≥400mAU/mL (P = 0.02) were independent prognostic factors.In the group of patients who met the criteria for small hepatocellular carcinoma,DCP≥400 mAU/mL was found to be an independent prognostic factor for recurrence-free (P = 0.02) and overall survival (P = 0.0005).In patients who did not meet the criteria,the presence of vascular invasion was an independent factor for recurrence-free (P = 0.02) and overall survivals (P = 0.01).In 75% of patients with small hepatocellular carcinoma and high DCP levels,recurrence occurred extrahepatically.CONCLUSION:For small hepatocellular carcinoma,a high preoperative DCP level appears indicative for tumor recurrence.Because many patients with a high preoperative DCP level develop extrahepatic recurrence,it is necessary to screen the whole body.

  9. Clinical evaluation of plasma abnormal prothrombin (PIVKA-II) in patients with hepatocellular carcinoma.

    Science.gov (United States)

    Fujiyama, S; Morishita, T; Sagara, K; Sato, T; Motohara, K; Matsuda, I

    1986-10-01

    The clinical usefulness of plasma abnormal prothrombin, defined as a protein induced by vitamin K absence or antagonist-II: PIVKA-II, as a tumor marker for hepatocellular carcinoma (HCC), was evaluated. Plasma PIVKA-II concentration was determined by an enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for PIVKA-II. Forty-one (65%) out of 63 patients with HCC had an abnormal PIVKA-II level above 0.13 arbitrary units (AU)/ml; the level was above 0.3 AU/ml in 33 patients (52%) and above 0.5 AU/ml in 27 patients (43%). On the other hand, most of the 282 patients with various liver diseases other than HCC had normal or slightly elevated levels of PIVKA-II. Their values were all below 0.5 AU/ml, with the exception of 2 patients with decompensated liver cirrhosis. The patients with PIVKA-II values above 0.5 AU/ml were strongly suspected of having HCC. Plasma PIVKA-II levels were not related to serum alpha-fetoprotein (AFP) levels, but were above 0.5 AU/ml in 14 (44%) out of the 32 patients whose serum AFP levels were below 400 ng/ml. In some patients with HCC, PIVKA-II was increased throughout the course of the disease, and in others it normalized after surgical resection of the tumor. We conclude that the plasma PIVKA-II assay by the ELISA method using a monoclonal antibody is a useful diagnostic tool for monitoring HCC, particularly in HCC patients with low AFP levels.

  10. Multicentric evaluation of a new assay for prothrombin fragment F1+2 determination.

    Science.gov (United States)

    Bruhn, H D; Conard, J; Mannucci, M; Monteagudo, J; Pelzer, H; Reverter, J C; Samama, M; Tripodi, A; Wagner, C

    1992-10-01

    A multicenter study of a recently developed ELISA for the determination of prothrombin fragment F1+2 was performed in order to evaluate analytical and clinical aspects. Mean intra-assay and inter-assay reproducibility were found to be 11.0 and 12.6%, respectively. The measuring range covered by the calibration curve reaches from 0.04 to 10.0 nM/l F1+2. Testing 133 healthy subjects a reference range of 0.37 to 1.11 nM/l F1+2 (2.5-97.5 percentile) with a median of 0.66 nM/l F1+2 was calculated. Minor difficulties with blood sampling (venous occlusion for 2 min) did not affect F1+2 plasma concentrations. Significantly increased F1+2 levels were measured in patients with leukemia (p < 0.0001), severe liver disease (p < 0.005) and after myocardial infarction (p < 0.01). Elevated F1+2 concentration before the beginning of heparin therapy (1.25 nM/l) decreased to 0.77 nM/l (p < 0.0001) after 1 day of therapy. For patients in the stable phase of oral anticoagulant therapy decreasing F1+2 concentrations were measured with increasing INR. F1+2 levels were already significantly reduced in patients with INR < 2.0 (0.56 nM/l; p = 0.0005). Thus F1+2 determination may be helpful in identifying activation processes as well as in monitoring anticoagulant therapy.

  11. Missense mutations in the gene encoding prothrombin corresponding to Arg596 cause antithrombin resistance and thrombomodulin resistance.

    Science.gov (United States)

    Takagi, Yuki; Murata, Moe; Kozuka, Toshihiro; Nakata, Yukiko; Hasebe, Ryo; Tamura, Shogo; Takagi, Akira; Matsushita, Tadashi; Saito, Hidehiko; Kojima, Tetsuhito

    2016-11-30

    Antithrombin (AT) and thrombomodulin (TM) play important roles in the process of natural anticoagulation in vivo. Recently, we reported that the prothrombin Yukuhashi mutation (p.Arg596Leu) was associated with AT and TM resistance-related thrombophilia. To assess the AT and TM resistances associated with other missense mutations by single base substitution in the Arg596 codon, we generated recombinant variants (596Gln, 596Trp, 596Gly, and 596Pro) and investigated the effects on AT and TM anticoagulant functions. All variants except 596Pro were secreted in amounts comparable to that of the wild-type but exhibited variable procoagulant activities. After a 30-minute inactivation by AT, the relative residual activity of wild-type thrombin decreased to 15 ± 4.0 %, in contrast to values of all variants were maintained at above 80 %. The thrombin-AT complex formation, as determined by enzyme-linked immunosorbent assay, was reduced with all tested variants in the presence and absence of heparin. In the presence of soluble TM (sTM), the relative fibrinogen clotting activity of wild-type thrombin decreased to 16 ± 0.12 %, whereas that of tested variants was 37 %-56 %. In a surface plasmon resonance assay, missense Arg596 mutations reduced thrombin-TM affinity to an extent similar to the reduction of fibrinogen clotting inhibition. In the presence of sTM or cultured endothelial-like cells, APC generation was enhanced differently by variant thrombins in a thrombin-TM affinity-dependent manner. These data indicate that prothrombin Arg596 missense mutations lead to AT and TM resistance in the variant thrombins and suggest that prothrombin Arg596 is important for AT- and TM-mediated anticoagulation.

  12. SwissProt search result: AK112119 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK112119 006-303-H09 (P98064) Complement-activating component of Ra-reactive factor... precursor (EC 3.4.21.-) (Ra-reactive factor serine protease p100) (RaRF) (Mannan-binding lectin serine prot...ease 1) [Contains: Complement-activating component of Ra-reactive factor MASP1_MOUSE 1e-19 ...

  13. SwissProt search result: AK109059 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK109059 002-154-F02 (P98064) Complement-activating component of Ra-reactive factor... precursor (EC 3.4.21.-) (Ra-reactive factor serine protease p100) (RaRF) (Mannan-binding lectin serine prot...ease 1) [Contains: Complement-activating component of Ra-reactive factor MASP1_MOUSE 5e-26 ...

  14. SwissProt search result: AK061101 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061101 006-207-A12 (P98064) Complement-activating component of Ra-reactive factor... precursor (EC 3.4.21.-) (Ra-reactive factor serine protease p100) (RaRF) (Mannan-binding lectin serine prot...ease 1) [Contains: Complement-activating component of Ra-reactive factor MASP1_MOUSE 2e-25 ...

  15. SwissProt search result: AK119378 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119378 001-132-A12 (P98064) Complement-activating component of Ra-reactive factor... precursor (EC 3.4.21.-) (Ra-reactive factor serine protease p100) (RaRF) (Mannan-binding lectin serine prot...ease 1) [Contains: Complement-activating component of Ra-reactive factor MASP1_MOUSE 1e-17 ...

  16. SwissProt search result: AK112005 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK112005 006-202-F10 (P98064) Complement-activating component of Ra-reactive factor... precursor (EC 3.4.21.-) (Ra-reactive factor serine protease p100) (RaRF) (Mannan-binding lectin serine prot...ease 1) [Contains: Complement-activating component of Ra-reactive factor MASP1_MOUSE 3e-19 ...

  17. Investigating Worldviews with Protégé

    DEFF Research Database (Denmark)

    Bro Wormslev Jakobsen, Thomas; Jakobsen, David; Øhrstrøm, Peter

    2016-01-01

    This paper offers a discussion of the usefulness of the ontology editor Protégé 5.0 as a tool for working with qualitative data. As an illustration we focus on an ongoing research project on the worldviews and beliefs of three groups of religious and spiritual professionals in Denmark. It is argued...

  18. SwissProt search result: AK102770 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK102770 J033107G10 (Q9Y6D6) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_HUMAN 4e-73 ...

  19. SwissProt search result: AK070145 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK070145 J023042O16 (O46382) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_BOVIN 9e-34 ...

  20. SwissProt search result: AK068209 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK068209 J013149H23 (Q9Y6D6) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_HUMAN 5e-52 ...

  1. SwissProt search result: AK102770 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK102770 J033107G10 (O46382) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_BOVIN 4e-72 ...

  2. SwissProt search result: AK240747 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK240747 J043039E11 (O46382) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_BOVIN 1e-58 ...

  3. SwissProt search result: AK068209 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK068209 J013149H23 (O46382) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_BOVIN 5e-51 ...

  4. SwissProt search result: AK070145 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK070145 J023042O16 (Q9Y6D6) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_HUMAN 1e-34 ...

  5. SwissProt search result: AK240747 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK240747 J043039E11 (Q9Y6D6) Brefeldin A-inhibited guanine nucleotide-exchange prot...ein 1 (Brefeldin A-inhibited GEP 1) (p200 ARF-GEP1) (p200 ARF guanine nucleotide exchange factor) BIG1_HUMAN 4e-58 ...

  6. EST2Prot: Mapping EST sequences to proteins

    Directory of Open Access Journals (Sweden)

    Lin David M

    2006-03-01

    Full Text Available Abstract Background EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked. Results We describe a system (EST2Prot that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss-Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors. Conclusion EST2Prot extends and significantly enriches the popular UniGene mapping by utilizing multiple relations between known biological entities. It produces a mapping between ESTs and proteins in real-time through a simple web-interface. The system is part of the Biozon database and is accessible at http://biozon.org/tools/est/.

  7. SwissProt search result: AK241918 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK241918 J075073G20 (Q9NRL2) Bromodomain adjacent to zinc finger domain protein 1A (ATP-utilizing chromatin... assembly and remodeling factor 1) (hACF1) (ATP-dependent chromatin remodelling prot...ein) (Williams syndrome transcription factor-related chromatin remodeli BAZ1A_HUMAN 2e-11 ...

  8. SwissProt search result: AK067393 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK067393 J013106B07 (Q9NRL2) Bromodomain adjacent to zinc finger domain protein 1A (ATP-utilizing chromatin... assembly and remodeling factor 1) (hACF1) (ATP-dependent chromatin remodelling prot...ein) (Williams syndrome transcription factor-related chromatin remodeli BAZ1A_HUMAN 2e-19 ...

  9. SwissProt search result: AK101745 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK101745 J033061O13 (Q9NRL2) Bromodomain adjacent to zinc finger domain protein 1A (ATP-utilizing chromatin... assembly and remodeling factor 1) (hACF1) (ATP-dependent chromatin remodelling prot...ein) (Williams syndrome transcription factor-related chromatin remodeli BAZ1A_HUMAN 3e-18 ...

  10. Differences in urinary prothrombin fragment 1 + 2 levels after total hip replacement in relation to venous thromboembolism and bleeding events

    DEFF Research Database (Denmark)

    Borris, L C; Breindahl, M; Lassen, M R;

    2008-01-01

    events. PATIENTS/METHODS: This study was conducted in parallel with a prospective, dose-finding study evaluating the efficacy and safety of different doses of rivaroxaban (Xarelto, Bayer HealthCare AG, Wuppertal, Germany) for thromboprophylaxis, relative to enoxaparin. Deep vein thrombosis was diagnosed......BACKGROUND: Prothrombin fragment 1 + 2 is excreted in urine (uF1 + 2) as a result of thrombin generation and, therefore, may be a useful marker of coagulation status. OBJECTIVES: To assess uF1 + 2 levels after total hip replacement (THR) in patients with venous thromboembolism (VTE) and bleeding...

  11. Kinetics of the activation of human prothrombin by human coagulation factor Xa. Initial rate studies in the presence of Ca2+ and phospholipid.

    Science.gov (United States)

    Kosow, D P; Orthner, C L

    1979-10-10

    Steady state kinetic studies have been performed to investigate the formation of thrombin from prothrombin by human coagulation Factor Xa in the presence of Ca2+ and phospholipid. The concentration of ligand which gives 50% of the maximum velocity (K0.5) is 2.3 mM for Ca2+, 7.4 microM for phospholipid, and 0.006 microM for prothrombin. Hill plots of the Ca2+ enhancement of the reaction give a Hill coefficient of 3.1, indicating positive cooperativity. The initial velocity patterns are consistent with an ordered addition of reactants with phospholipid as the second reactant to bind to the enzyme. Although our results do not differentiate between Ca2+ or the prothrombin substrate as the first reactant to bind to Factor Xa, it is established that Ca2+ can bind to Factor Xa in the absence of the other reactants. Thus, the most probable order of addition of reactants is Ca2+, phospholipid, and the prothrombin substrate. Plots of (v)-1 versus (prothrombin)-1 or (v)-1 versus [(Ca2+)3]-1 at several constant concentrations of phospholipid indicate that the major effect of phospholipid is to increase the turnover number of Factor Xa.

  12. Homozygous factor V Leiden and double heterozygosity for factor V Leiden and prothrombin mutation.

    Science.gov (United States)

    Saemundsson, Ymir; Sveinsdottir, Signý Vala; Svantesson, Henrik; Svensson, Peter J

    2013-10-01

    The most common forms of familial thrombophilia are factor V Leiden (FVL) and prothrombin mutation (PTM). Homozygous FVL and PTM have long been feared conditions thought to cause high rates of morbidity and mortality. To analyse clinical features in patients with homozygous FVL and PTM, as well as patients with double heterozygosity for FVL and PTM. All patients with homozygous FVL, PTM or double heterozygosity in the MATS database of 1465 consecutive unselected patients were analysed regarding age at inclusion venous thromboembolism (VTE), age at first thrombosis, recurrence, clinical course and acquired risk factors. We found 36 patients homozygous for FVL. Patients homozygous for FVL were younger than controls at group level (56 ± 18 vs. 63 ± 17, p < 0.02). Homozygous women were younger than female controls (50 ± 19 vs. 63 ± 18, p < 0.002). No difference was observed when comparing male subjects. Women were younger than men at inclusion thrombosis (50 ± 19 vs. 65 ± 14, p < 0.02) and at first thrombosis (47 ± 19 vs. 64 ± 14, p < 0.01). Deep venous thrombosis (DVT) was seen in 33 patients (92 %), 6 (17 %) had pulmonary embolism (PE) and 3 (8 %) had combined DVT and PE. PE was less frequent in homozygous FVL women compared to female controls (p < 0.03). VTE recurred in 3 subjects during the duration of the study. Odds ratio for VTE in homozygous FVL patients compared to controls was 13.9 (95 % CI 9.9-19.7). We found no subjects with homozygous PTM. Double heterozygosity for FVL and PTM was seen in 12 subjects. There was no difference in age at inclusion VTE between double heterozygotes and controls (59 ± 16 vs. 63 ± 17, ns.). DVT was seen in 92 % at inclusion, 8 % had PE. Mean age at first VTE was 52 ± 17 (27-82). Consecutive homozygous FVL patients had a higher age at first thrombosis than previously described. Homozygous females are affected at an earlier age than homozygous men and female controls. It seems that

  13. Co-­Protégé: Collaborative Ontology Building with Divergences

    OpenAIRE

    2006-01-01

    http://ieeexplore.ieee.org/; In this paper we present an innovative approach to develop a domain ontology in collaborative fashion. This approach is synthesized in a groupware application which is called Co-Protégé, a set of plug­ins which extends Protégé. This approach is innovative because Co­Protégé enables the coexistence of divergent conceptualizations and the discussion thread in order to record the ontology evolution.

  14. Overview of transcriptomic analysis of all human proteases, non-proteolytic homologs and inhibitors: Organ, tissue and ovarian cancer cell line expression profiling of the human protease degradome by the CLIP-CHIP™ DNA microarray.

    Science.gov (United States)

    Kappelhoff, Reinhild; Puente, Xose S; Wilson, Claire H; Seth, Arun; López-Otín, Carlos; Overall, Christopher M

    2017-08-07

    The protease degradome is defined as the complete repertoire of proteases and inhibitors, and their nonfunctional homologs present in a cell, tissue or organism at any given time. We review the tissue distribution of virtually the entire degradome in 23 different human tissues and 6 ovarian cancer cell lines. To do so, we developed the CLIP-CHIP™, a custom microarray based on a 70-mer oligonucleotide platform, to specifically profile the transcripts of the entire repertoire of 473 active human proteases, 156 protease inhibitors and 92 non-proteolytically active homologs known at the design date using one specific 70-mer oligonucleotide per transcript. Using the CLIP-CHIP™ we mapped the expression profile of proteases and their inhibitors in 23 different human tissues and 6 ovarian cancer cell lines in 104 sample datasets. Hierarchical cluster analysis showed that expression profiles clustered according to their anatomic locations, cellular composition, physiologic functions, and the germ layer from which they are derived. The human ovarian cancer cell lines cluster according to malignant grade. 110 proteases and 42 inhibitors were tissue specific (1 to 3 tissues). Of these 110 proteases 69% (74) are mainly extracellular, 30% (34) intracellular and 1% intramembrane. Notably, 35% (197/565) of human proteases and 30% (47/156) of inhibitors were ubiquitously expressed in all 23 tissues; 27% (155) of proteases and 21% (32) of inhibitors were broadly expressed in 4-20 tissues. Our datasets provide a valuable resource for the community of baseline protease and inhibitor relative expression in normal human tissues and can be used for comparison with diseased tissue, e.g. ovarian cancer, to decipher pathogenesis, and to aid drug development. This article is part of a Special Issue entitled: Proteolysis as a Regulatory Event in Pathophysiology edited by Stefan Rose-John. Crown Copyright © 2017. Published by Elsevier B.V. All rights reserved.

  15. Preoperative screening for coagulopathy using prothrombin time and partial thromboplastin time in patients requiring primary cranial vault remodeling.

    Science.gov (United States)

    Genecov, David G; Por, Yong-Chen; Barcelo, Carlos Raul; Salyer, Kenneth E; Mulne, Arlynne F; Morad, Ammar B

    2005-08-01

    The aim of this study was to investigate the prevalence of abnormal preoperative screening prothrombin time and partial thromboplastin time in patients listed for primary cranial vault remodeling that required hematologic workup and their diagnoses and subsequent management. This retrospective analysis was performed from January of 2000 to December of 2003 at the International Craniofacial Institute, Dallas, Texas, on a total of 168 patients. All patients had a normal prothrombin time. Abnormally raised partial thromboplastin time was found in six patients (prevalence of 3.57 percent), one who had factor XI deficiency, one who had a borderline factor XI deficiency and circulating inhibitor, one who had an intermittent factor XI deficiency and circulating inhibitor, one who had a borderline von Willebrand's disease with low factor XII, and the remaining two who had a circulating inhibitor of coagulation. Of these six patients, the perioperative management was altered in four of five patients, and one patient declined surgery out of fear of surgical morbidity. The surgery of one patient was aborted intraoperatively because of abnormal bleeding without clot formation after the calvarial burr holes had been drilled. The mean blood loss was 183 ml for the four patients with completed surgery and 100 ml for one patient. The authors conclude that even though the prevalence of abnormal screening partial thromboplastin time in these patients was low (3.57 percent), detection of an abnormal result required preoperative correction of coagulopathy in 80 percent of cases.

  16. Antibodies Against Annexin V and Prothrombin, Their Correlation with Other Anti-phospholipid Antibodies in Recurrent Pregnancy Loss

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Objective To study the findings of serum antibodies against annexin V, prothrombin,ph-inositol, ph-acid, ph-ethanolamine, ph-serine, ph-glycerol, cardiolipin, and beta2-glycoprotein I and analyze the trophoblast annexin V receptorsMethods Sera from 156 patients aged 26-41 years with recurrent pregnancy loss (3-7 times) were investigated. Eighty-four fertile healthy women aged 24-38 years were included in a control group. ELISA methods were used for detecting a panel of sera anti-phospholipid antibodies. Immunolocalization of annexin Vreceptors in 143trophoblast specimens of 156patients was investigated by the immunofluorescence technique using Annexin V-FITC, Apoptosis and Annexin V-CY3 commercial kits.Results Positivity for anti-phospholipid antibodies mainly against ph-serine, phethanolamine, and ph-inositol was found together in 80. 8% (126 out of 156 patients),anti-prothrombin antibodies in 12% (18), and anti-annexin Vantibodies in 13. 5%(21) women. No significant levels of anti-phospholipid antibodies were found in 6controls. Placenta immunohistopathology also exhibited some changes manifested by the presence of apoptotic and necrotic cells in trophoblast, and very few microthrombotization in some intervillous spaces.Conclusion Our detailed study demonstrated the prevalence of majority of antiphospholipid antibodies as a high risk factor for repeated reproductive failure. Very low microthrombosis in placentas could be explained by the changes of haemocoagulation properties out of uterus.

  17. Serum des-R prothrombin activation peptide fragment 2: a novel prognostic marker for disseminated intravascular coagulation.

    Science.gov (United States)

    Chung, Soie; Kim, Ji-Eun; Kim, Hyun Kyung; Yeon, Eun Hee; Shin, Yong Sung; Kim, Chul Woo

    2013-06-01

    Disseminated intravascular coagulation (DIC) is diagnosed based on the combination of predisposing underlying conditions and laboratory tests for plasma coagulation markers. Because the collection of blood plasma samples is a fastidious procedure, the serum sample method may be preferred for measurement of coagulation markers when feasible. The novel serum marker des-R prothrombin activation peptide fragment 2 (des-R F2) was measured using a sandwich enzyme-linked immunosorbent assay in 181 patients suspected of having DIC. Thrombin generation potential was estimated with a calibrated automated thrombogram. Serum des-R F2 was generated with an in vitro clotting process within a serum separation tube after blood collection. Carboxypeptidase inhibitor inhibited the formation of des-R F2 during in vitro clotting. Low levels of prothrombin and thrombin generation potential resulted in low serum des-R F2 levels. Serum des-R F2 was significantly decreased in overt DIC. Levels of des-R F2 correlated with DIC severity and other coagulation markers. Of note, the decrease in serum des-R F2 levels was a significant marker for predicting mortality. The serum marker, des-R F2, can be used for the investigation of DIC severity and prognosis. It should be considered a useful marker, especially when only serum samples are available. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Comparison of prothrombin time tests used in the monitoring of edoxaban and their evaluation as indicators of the reversal effect.

    Science.gov (United States)

    Iba, Toshiaki; Emmi, Mari; Hiki, Makoto; Nagayama, Masataka; Aihara, Koichiro; Tabe, Yoko; Yuri, Maiko; Ohsaka, Akimichi

    2016-06-01

    Clinical demand for the prompt assessment of the activity of direct-acting factor Xa inhibitors in the emergency care setting is increasing. In the present study, we examined whether prothrombin time (PT) tests can serve as a clinically useful indicator of anti-factor Xa activity. In the first series, the in vitro effect of edoxaban on PT was evaluated by spiking human plasma with edoxaban and measuring PT using three different commercial PT tests. In the second series, the reversal effect of prothrombin complex concentrates (PCC) and activated PCC (aPCC) in edoxaban-spiked plasma was evaluated. In the third series, PT of plasma samples from patients administered either 15 or 30 mg/day of edoxaban was assessed, and the results were compared with edoxaban concentrations determined by a calibrated anti-factor Xa activity assay. The spike test revealed that all PT reagents positively correlated with edoxaban. The sensitivity to edoxaban varied among the three reagents and Triniclot(®) Excel S showed the best performance. Prolonged PT by edoxaban was reversed by PCC and aPCC in a dose-dependent manner; however, complete reversal was not achieved. Positive correlation between anti-factor Xa activity and PT was shown in the clinical samples at the edoxaban range from 0 to >300 ng/mL.

  19. Effective reversal of edoxaban-associated bleeding with four-factor prothrombin complex concentrate in a rabbit model of acute hemorrhage.

    Science.gov (United States)

    Herzog, Eva; Kaspereit, Franz; Krege, Wilfried; Doerr, Baerbel; Mueller-Cohrs, Jochen; Pragst, Ingo; Morishima, Yoshiyuki; Dickneite, Gerhard

    2015-02-01

    Edoxaban is an oral, selective direct factor Xa inhibitor approved in Japan for venous thromboembolism prevention after orthopedic surgery. Data are lacking regarding reversal strategies for edoxaban; this study assessed whether four-factor prothrombin complex concentrate (Beriplex/Kcentra; CSL Behring GmbH, Marburg, Germany) can effectively reverse its effects on hemostasis using a previously described rabbit model. The study comprised assessments of thrombin generation in vitro, pharmacokinetic parameters, and edoxaban reversal in vivo. In a blinded in vivo stage, a standardized kidney incision was performed in animals (n = 11 per group) randomized to receive vehicle + saline, edoxaban (1,200 μg/kg) + saline, or edoxaban (1,200 μg/kg) + four-factor prothrombin complex concentrate (50 IU/kg). Animals were monitored for treatment impact on hemostasis and coagulation parameters. Data are median (range). Statistical tests were adjusted for multiple testing. Edoxaban administration increased blood loss (30 [2 to 44] ml) and time to hemostasis (23 [8.5 to 30.0] min) compared with the control group (3 [1 to 8] ml and 3 [2.0 to 5.0] min, respectively). Biomarkers of coagulation (prothrombin time, activated partial thromboplastin time, whole blood clotting time) and thrombin generation parameters (e.g., peak thrombin, endogenous thrombin potential, lag time) were also affected by edoxaban. Administration of four-factor prothrombin complex concentrate significantly reduced time to hemostasis (to 8 [6.5 to 14.0] min, observed P < 0.0001) and total blood loss (to 9 [4 to 22] ml, observed P = 0.0050) compared with the edoxaban + saline group. Of the biomarkers tested, prothrombin time, whole blood clotting time, and endogenous thrombin potential correlated best with clinical parameters. In a rabbit model of hemostasis, four-factor prothrombin complex concentrate administration significantly decreased edoxaban-associated hemorrhage.

  20. The effect of the administration of vitamin K2 to the pregnant women on the activities of prothrombin group in cord blood.

    OpenAIRE

    2015-01-01

    Background: Hemorrhagic disease of the newborn (HDN) is a hemorrhage at the neonatal period. The most dangerous form of HDN is intracranial bleeding which may be fatal. The most frequent cause of HDN is deficiency of vitamin K dependent factors or prothrombin group.Objective: The aim of the study is to know the effect of the administration of vitamin K2 to the pregnant women on the activities of prothrombin gourp in cord blood.Methods: This was experimental design. Forty pregnant women were e...

  1. Three versus four-factor prothrombin complex concentrates for "factor-based" resuscitation in a porcine hemorrhagic shock model.

    Science.gov (United States)

    Moe, Donald Michael; Lallemand, Michael Scott; McClellan, John Mason; Smith, Joshua Porter; Marko, Shannon T; Eckert, Matthew J; Martin, Matthew J

    2017-07-12

    Bleeding is a leading cause of preventable death following severe injury. Prothrombin complex concentrates (PCC) treat inborn coagulation disorders and reverse oral anticoagulants, but are proposed for use in "factor-based" resuscitation strategies. Few studies exist for this indication in acidosis, or that compare 3-factor (3PCC) versus 4-factor (4PCC) products. We aimed to assess and compare their safety and efficacy in a porcine model of severe hemorrhagic shock and coagulopathy. Twenty-five adult Yorkshire swine underwent 35% volume hemorrhage, ischemia-reperfusion injury, and protocolized crystalloid resuscitation. Seventeen animals were randomized at 4 hours following model creation to receive a 45-IU/kg dose of either 3PCC or 4PCC. An additional 8 animals received autologous plasma transfusion prior to 4PCC to better characterize response to PCC. Individual factor levels were drawn at 4 and 6 hours. The model created significant acidosis with mean pH 7.21 and lactate of 9.6 mmol/L. Following PCC, 66.7% of 3PCC animals and 25% of 4PCC animals (regardless of plasma administration) developed consumptive coagulopathy. The animals that developed consumptive coagulopathy had manifested the "lethal triad" with lower temperatures (36.3 vs. 37.8°C), increased acidosis (pH 7.14 vs. 7.27, base excess -12.1 vs. -6.5 mEq/L), and worse coagulopathy (prothrombin time 17.1 vs. 14.6 seconds, fibrinogen 87.9 vs. 124.1 mg/dL) (all pfactors with transient improvement of prothrombin time, but there was significant depletion of fibrinogen and platelets with no lasting improvement of coagulopathy. PCC failed to correct coagulopathy and was associated with fibrinogen and platelet depletion. Of greater concern, PCC administration resulted in consumptive coagulopathy in the more severely ill animals. The incidence of consumptive coagulopathy was markedly increased with 3PCC versus 4PCC, and these products should be used with caution in this setting. II, therapeutic.

  2. LassoProt: server to analyze biopolymers with lassos.

    Science.gov (United States)

    Dabrowski-Tumanski, Pawel; Niemyska, Wanda; Pasznik, Pawel; Sulkowska, Joanna I

    2016-07-01

    The LassoProt server, http://lassoprot.cent.uw.edu.pl/, enables analysis of biopolymers with entangled configurations called lassos. The server offers various ways of visualizing lasso configurations, as well as their time trajectories, with all the results and plots downloadable. Broad spectrum of applications makes LassoProt a useful tool for biologists, biophysicists, chemists, polymer physicists and mathematicians. The server and our methods have been validated on the whole PDB, and the results constitute the database of proteins with complex lassos, supported with basic biological data. This database can serve as a source of information about protein geometry and entanglement-function correlations, as a reference set in protein modeling, and for many other purposes.

  3. 48 CFR 1019.202-70-8 - Protégé firms.

    Science.gov (United States)

    2010-10-01

    ... SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 1019.202-70-8 Protégé firms. (a) For selection as a protégé, a firm must be: (1) A small business, women-owned small business, small disadvantaged business, small business owned and controlled by service disabled veterans, or qualified HUBZone small......

  4. ChemProt-3.0: a global chemical biology diseases mapping

    DEFF Research Database (Denmark)

    Kringelum, Jens Vindahl; Kjærulff, Sonny Kim; Brunak, Søren

    2016-01-01

    ChemProt is a publicly available compilation of chemical-protein-disease annotation resources that enables the study of systems pharmacology for a small molecule across multiple layers of complexity from molecular to clinical levels. In this third version, ChemProt has been updated to more than 1...

  5. Les apports nutritionnels conseillés (ANC en protéines

    Directory of Open Access Journals (Sweden)

    Patureau mirand Philippe

    2003-01-01

    Full Text Available La définition d’apports conseillés en protéines repose sur l’analyse des besoins protéiques. Un ANC avec comme objectif mesuré le maintien de la masse des protéines corporelles, a été fixé à 0,8 g de protéines de bonne qualité par kg de poids et par jour. Toutefois le besoin protéique est beaucoup plus complexe en raison de la diversité de la nature des nutriments requis pour le satisfaire (azote, acides aminés, peptides, protéines et de la multiplicité de leurs fonctions. L’ANC en protéines intègre en grande partie cette diversité et constitue un repère simple qui permet de s’assurer globalement de la couverture des besoins protéiques d’une large population. Sa simplicité en limite cependant la pertinence pour des sous-populations ayant des besoins plus spécifiques.

  6. Attachment security, the quality of the mentoring relationship and protégés' adjustment.

    Science.gov (United States)

    Goldner, Limor; Scharf, Miri

    2014-08-01

    Associations among protégés' attachment orientations, the quality of their mentoring relationships, and mentoring outcomes were explored within the context of an Israeli national community-based mentoring program (Perach) comprising 167 protégés (47 % females, mean age 9.6). Contrary to expectations, we did not find a correlation between protégés' level of attachment security and the quality of their mentoring relationship. However, the findings indicated associations between a positive mentoring relationship and protégés' adjustment at the end of mentoring. Moreover, protégés' attachment security moderated the associations between the quality of their mentoring relationships and their adjustment at the end of mentoring. Among secure protégés, a positive mentoring relationship was more positively associated with general self-concept than among insecure protégés. Although insecure protégés' positive mentoring relationships were negatively associated with loneliness at the end of mentoring, these relationships were positively associated among insecure individuals. The theoretical and practical implications for mentoring interventions are discussed.

  7. UniProt search blastx result - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available t UNIPROT ID UniProt ID DEFINITION Definition of the protein in the UniProt datab...full-length cDNA clone CLONE_NAME Name of the full-length cDNA clone RANK Ranking in the blastx search resul

  8. An observational, prospective, two-cohort comparison of a fixed versus variable dosing strategy of prothrombin complex concentrate to counteract vitamin K antagonists in 240 bleeding emergencies

    NARCIS (Netherlands)

    Khorsand, Nakisa; Veeger, Nic J. G. M.; van Hest, Reinier M.; Ypma, Paula F.; Heidt, Jeroen; Meijer, Karina

    2012-01-01

    Background Despite years of experience with vitamin K antagonist-associated bleeding events, there is still no evidence to help identify the optimal treatment with prothrombin complex concentrates. Variable dosing and fixed dose strategies are being used. In this observational prospective two-cohort

  9. Risk of Recurrent Venous Thrombosis in Homozygous Carriers and Double Heterozygous Carriers of Factor V Leiden and Prothrombin G20210A

    NARCIS (Netherlands)

    Lijfering, Willem M.; Middeldorp, Saskia; Veeger, Nic J. G. M.; Hamulyak, Karly; Prins, Martin H.; Bueller, Harry R.; van der Meer, Jan

    2010-01-01

    Background-Homozygous or double heterozygous factor V Leiden and/or prothrombin G20210A is a rare inherited thrombophilic trait. Whether individuals with this genetic background have an increased risk of recurrent venous thrombosis is uncertain. Methods and Results-A case-control design within a lar

  10. Risk of Budd-Chiari syndrome associated with factor V Leiden and G20210A prothrombin mutation: a meta-analysis.

    Directory of Open Access Journals (Sweden)

    Peijin Zhang

    Full Text Available BACKGROUND: Various studies have demonstrated that factor V Leiden (FVL and G20210A prothrombin mutation contribute to the risk of Budd-Chiari syndrome (BCS, while other studies provided conflicting findings. In order to derive more precise estimations of the relationships, a meta-analysis was performed. METHODS: Eligible articles were identified through search of databases including Pubmed, Chinese Biomedical Database (CBM, Chinese, and Chinese National Knowledge Infrastructure (CNKI, Chinese. Odd ratios (ORs with 95% confidence intervals (CIs were calculated using random- or fixed- model. RESULTS: Finally, twelve studies were included for FVL and nine studies were included for G20210A prothrombin mutation. With respect to FVL, significantly increased BCS risk was found in the overall population (OR = 6.29, 95%CI = 4.23-9.36. Subgroup analyses suggested that FVL was associated with an increased risk of BCS in the population with high background mutation prevalence (>1% in the normal population. No significant association was found between BCS and G20210A prothrombin mutation (OR = 1.78, 95%CI = 0.77-4.11. CONCLUSION: The presence of FVL should be evaluated in patients with BCS. Conversely, G20210A prothrombin mutation is not significantly associated with risk of BCS. Large-scale well designed studies are necessary to be conducted to further confirm or refute the observed association.

  11. Factor V Leiden 1691G/A and prothrombin gene 20210G/A polymorphisms as prothrombotic markers in adult Egyptian acute leukemia patients.

    Science.gov (United States)

    El Sissy, Azza Hamdy; El Sissy, Maha H; Elmoamly, Shereef

    2014-11-01

    Factor V Leiden 1691G/A and prothrombin gene 20210G/A mutations are the most common genetic defects leading to thrombosis. This work aimed to study the FV Leiden and the prothrombin gene polymorphism in adult Egyptian patients with acute leukemia and their importance in thrombophilia screening. The study included 76 patients with acute leukemia and 100 healthy controls. Genotyping was done by real-time polymerase chain reaction technique. For factor V Leiden, the frequency of G/A mutation conferred more than 2.5-fold of increased risk of (OR 2.639 95 % CI 1.045-6.669). The frequency of factor V Leiden combined (G/A + A/A) genotypes conferred 2.83-fold of increased risk (OR 2.828, CI 1.13-7.075), The A allele conferred almost threefold increased risk (OR 2.824, 95 % CI 1.175-6.785). Despite higher frequency in patients compared to controls, there was no risk of association between prothrombin gene mutation and acute leukemia in adult Egyptians nor was there between combined genotypes of prothrombin gene mutation and factor V Leiden.

  12. Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T

    DEFF Research Database (Denmark)

    Simone, Benedetto; De Stefano, Valerio; Leoncini, Emanuele

    2013-01-01

    Genetic and environmental factors interact in determining the risk of venous thromboembolism (VTE). The risk associated with the polymorphic variants G1691A of factor V (Factor V Leiden, FVL), G20210A of prothrombin (PT20210A) and C677T of methylentetrahydrofolate reductase (C677T MTHFR) genes ha...

  13. The Effect of Mentoring on Protégés' Organizational Deviance.

    Science.gov (United States)

    Chen, Cheng; Wen, Peng

    2016-08-01

    The aim of the present study was to investigate the effect of mentoring on protégés' organizational deviance. The sample comprised 202 ongoing formal mentoring dyads in the People's Republic of China (mentor samples: 61.9% male, M age = 36.8 years; protégé samples: 57.4% male, M age = 25.0 years). The regression results showed that mentoring was negatively related to protégés' organizational deviance. Moreover, job embeddedness and organizational identification mediated the association between mentoring and protégés' organizational deviance. Furthermore, the perceived developmental climate played a significant moderating role in the relationships between mentoring and job embeddedness and organizational identification such that the relationships were stronger when protégés perceived a stronger developmental climate. The theoretical and practical implications of this study were discussed.

  14. The neXtProt knowledgebase on human proteins: 2017 update

    Science.gov (United States)

    Gaudet, Pascale; Michel, Pierre-André; Zahn-Zabal, Monique; Britan, Aurore; Cusin, Isabelle; Domagalski, Marcin; Duek, Paula D.; Gateau, Alain; Gleizes, Anne; Hinard, Valérie; Rech de Laval, Valentine; Lin, JinJin; Nikitin, Frederic; Schaeffer, Mathieu; Teixeira, Daniel; Lane, Lydie; Bairoch, Amos

    2017-01-01

    The neXtProt human protein knowledgebase (https://www.nextprot.org) continues to add new content and tools, with a focus on proteomics and genetic variation data. neXtProt now has proteomics data for over 85% of the human proteins, as well as new tools tailored to the proteomics community. Moreover, the neXtProt release 2016-08-25 includes over 8000 phenotypic observations for over 4000 variations in a number of genes involved in hereditary cancers and channelopathies. These changes are presented in the current neXtProt update. All of the neXtProt data are available via our user interface and FTP site. We also provide an API access and a SPARQL endpoint for more technical applications. PMID:27899619

  15. Mesenteric vein thrombosis in a patient heterozygous for factor V Leiden and G20210A prothrombin genotypes.

    Science.gov (United States)

    Karmacharya, Paras; Aryal, Madan Raj; Donato, Anthony

    2013-11-21

    Mesenteric venous thrombosis (MVT) is a rare but life threatening form of bowel ischemia. It is implicated in 6%-9% of all cases of acute mesenteric ischemia. The proportion of patients with primary (or idiopathic) MVT varies from 0% to 49%, with a decrease in frequency secondary to more recent availability of newer investigations for hypercoagulability. The presence of factor V Leiden (FVL) and prothrombin G20210A mutations (PGM) have been well documented in these cases. However, there have been scarce case reports describing MVT in heterozygotes of both these mutations occurring simultaneously and its implications on long term management. Our case describes acute MVT in a previously asymptomatic young patient with no prior history of venous thromboembolism. The patient was found to be heterozygous for FVL and PGM and treated with lifelong anticoagulation with warfarin (goal international normalized ratio: 2-3) and avoidance of hormonal contraceptives.

  16. Des-gamma-carboxy prothrombin (PIVKA-II)-producing mediastinal embryonal carcinoma with features of hepatoid differentiation.

    Science.gov (United States)

    Hasegawa, Yasuyuki; Tomita, Katsuyuki; Hashimoto, Kiyoshi; Shigeoka, Yasushi; Watanabe, Masanari; Yamasaki, Akira; Shimizu, Eiji

    2005-01-01

    The case of a 48-year-old man with primary nonseminomatous embryonal carcinoma at the posterior mediastinum is described. The patient displayed extremely high plasma levels of Des-gamma-carboxy prothrombin (PIVKA-II) (4040 mAU/ml). Ultrasonography and dynamic computed tomography ruled out hepatocellular carcinoma (HCC) or liver metastasis. After preoperative systemic chemotherapy, total tumor resection was performed. Postoperatively, the plasma levels of PIVKA-II returned to within the normal range (24 mAU/ml). An immnohistochemical study using anti-PIVKA-II monoclonal antibody revealed the cytoplasmic expression of PIVK4-II in the carcinoma cells. These results indicate that tumor cells, which are manifested as hepatoid differentiation, may produce PIVKA-II. This case seems to be the first case reported in which PIVKA-II was produced by nonseminomatous mediastinal embryonal carcinoma without HCC or liver metastasis.

  17. Clinical significance of factor V leiden and prothrombin G20210A-mutations in cerebral venous thrombosis - comparison with arterial ischemic stroke.

    Science.gov (United States)

    Beye, Aida; Pindur, Gerhard

    2017-08-28

    Cerebrovascular diseases are considered in a different way concerning their etiology with regard to arterial and venous occlusion. The role of thrombophilia in this context remains undetermined. For this reason, a case-control study was conducted including a total of 202 patients (154 females, 48 males) aged from 18 to 76 years (mean: 39.8 years) suffering either from cerebral sinus venous thrombosis (n = 101) or from arterial ischemic stroke (n = 101). Study groups were evaluated on the basis of age- and gender-matched pairs. Gene mutations of factor V-1691 (factor V Leiden) and prothrombin-20210 being considered as the most common thrombophilia markers were analyzed in this study. Factor V Leiden-mutations were found in 16.8% of patients with cerebral sinus venous thrombosis (CVT) and in 17.8% of patients with arterial ischemic stroke (AIS), which was significantly more frequent than in controls at a rate of 4.95% (ORs: 3.89 and 4.16). Prothrombin-mutations were significantly more frequent in CVT at a rate of 14.9% versus 2.97% in controls (OR: 5.70). This does not apply for AIS showing a rate of 4.95% prothrombin-mutations. Rates of factor V Leiden-mutations are not different in CVT compared with AIS. In contrast, however, prothrombin-mutations were significantly more frequent in CVT than in AIS with a rate of 14.9% versus 4.95% (OR 3.35). Furthermore, 3 cases with combined heterozygosity of factor V Leiden- and prothrombin-mutation have been identified in CVT, but not in AIS or controls. All of the above mentioned mutations were exclusively heterozygous. We conclude from these data that thrombophilia in terms of factor V Leiden genotype is a risk factor for both CVT and AIS in equal measure. In contrast, prothrombin-20210-mutations were different playing a significant role in the pathogenesis of cerebral sinus vein- thrombosis, but not in arterial ischemic stroke. Also, the combined occurrence of heterozygous prothrombin- and factor V Leiden

  18. A novel prothrombin time method to measure all non-vitamin K-dependent oral anticoagulants (NOACs).

    Science.gov (United States)

    Lindahl, Tomas L; Arbring, Kerstin; Wallstedt, Maria; Rånby, Mats

    2017-09-11

    There is a clinical need for point-of-care (POC) methods for non-vitamin K-dependent oral anticoagulants (NOACs). We modified a routine POC procedure: Zafena's Simple Simon™ PT-INR, a room-temperature, wet-chemistry prothrombin time method of the Owren-type. To either increase or decrease NOAC interference, two assay variants were devised by replacing the standard 10 µL end-to-end capillary used to add the citrated plasma sample to 200 µL of prothrombin time (PT) reagent by either a 20 µL or a 5 µL capillary. All assay variants were calibrated to show correct PT results in plasma samples from healthy and warfarin-treated persons. For plasmas spiked with dabigatran, apixaban, or rivaroxaban, the 20 µL variant showed markedly higher PT results than the 5 µL. The effects were even more pronounced at room temperature than at +37 °C. In plasmas from patients treated with NOACs (n = 30 for each) there was a strong correlation between the PT results and the concentration of NOACs as determined by the central hospital laboratory. For the 20 µL variant the PT response of linear correlation coefficient averaged 0.90. The PT range was INR 1.1-2.1 for dabigatran and apixaban, and INR 1.1-5.0 for rivaroxaban. Using an INR ratio between the 20 µL and 5 µL variants (PTr20/5) made the NOAC assay more robust and independent of the patient sample INR value in the absence of NOAC. Detection limits were 80 µg/L for apixaban, 60 µg/L for dabigatran, and 20 µg/L for rivaroxaban. A wet-chemistry POC PT procedure was modified to measure the concentrations of three NOACs using a single reagent.

  19. Phenotypic presentation of thrombophilia in double heterozygote for factor v leiden and prothrombin 20210 G>A mutations: Case report

    Directory of Open Access Journals (Sweden)

    Nagorni-Obradović Ljudmila

    2014-01-01

    Full Text Available Physicians usually do not suspect pulmonary thromboembolism in younger patients except in those who have thrombophilia. In those latter patients some special conditions such as trauma or surgery may provoke the disease. In some adult persons, thrombophilia may still remain unrecognized, until appearance of additional conditions influence development of thrombosis. A 55-year-old Caucasian female, non-smoker, experienced sudden chest pain and hemoptysis without chest trauma. History taking revealed type 2 diabetes mellitus and hypothyroidism. She was overweight with body mass index 29.0. The review of the family history revealed that her father and mother died of brain infarction, while her 22-year-old son and 24-year-old daughter were healthy. Due to suspicion for thrombosis, multi-slice computerized tomography thorax scan was done and pulmonary embolism was diagnosed. Although without clear risk factor for thrombosis in our patient, we performed laboratory investigation for congenital thrombophilia. Genetic analysis showed double heterozygous for factor V Leiden and prothrombin 20210 G>A mutations. Congenital thrombophilia was risk factor for thrombosis in our patient but haemostatic imbalance was not previously clinically recognized. She had two pregnancies without complications. Appearance of other associative factors such as endocrine disorders - hypothyroidism and metabolic syndrome with diabetes type 2, and overweigh were additional potential triggers for clinical manifestation of pulmonary thromboembolism in her adult age. Her children underwent genetic analysis, too. The son was also double heterozygous for factor V Leiden and prothrombin 20210 G>A mutations, while daughter was heterozygous for factor V Leiden, and none had clinical signs of thrombosis. [Projekat Ministarstva nauke Republike Srbije, br. ON175081 i br. ON 175091

  20. DeltaProt: a software toolbox for comparative genomics

    Directory of Open Access Journals (Sweden)

    Willassen Nils P

    2010-11-01

    Full Text Available Abstract Background Statistical bioinformatics is the study of biological data sets obtained by new micro-technologies by means of proper statistical methods. For a better understanding of environmental adaptations of proteins, orthologous sequences from different habitats may be explored and compared. The main goal of the DeltaProt Toolbox is to provide users with important functionality that is needed for comparative screening and studies of extremophile proteins and protein classes. Visualization of the data sets is also the focus of this article, since visualizations can play a key role in making the various relationships transparent. This application paper is intended to inform the reader of the existence, functionality, and applicability of the toolbox. Results We present the DeltaProt Toolbox, a software toolbox that may be useful in importing, analyzing and visualizing data from multiple alignments of proteins. The toolbox has been written in MATLAB™ to provide an easy and user-friendly platform, including a graphical user interface, while ensuring good numerical performance. Problems in genome biology may be easily stated thanks to a compact input format. The toolbox also offers the possibility of utilizing structural information from the SABLE or other structure predictors. Different sequence plots can then be viewed and compared in order to find their similarities and differences. Detailed statistics are also calculated during the procedure. Conclusions The DeltaProt package is open source and freely available for academic, non-commercial use. The latest version of DeltaProt can be obtained from http://services.cbu.uib.no/software/deltaprot/. The website also contains documentation, and the toolbox comes with real data sets that are intended for training in applying the models to carry out bioinformatical and statistical analyses of protein sequences. Equipped with the new algorithms proposed here, DeltaProt serves as an auxiliary

  1. enDNA-Prot: Identification of DNA-Binding Proteins by Applying Ensemble Learning

    Directory of Open Access Journals (Sweden)

    Ruifeng Xu

    2014-01-01

    Full Text Available DNA-binding proteins are crucial for various cellular processes, such as recognition of specific nucleotide, regulation of transcription, and regulation of gene expression. Developing an effective model for identifying DNA-binding proteins is an urgent research problem. Up to now, many methods have been proposed, but most of them focus on only one classifier and cannot make full use of the large number of negative samples to improve predicting performance. This study proposed a predictor called enDNA-Prot for DNA-binding protein identification by employing the ensemble learning technique. Experiential results showed that enDNA-Prot was comparable with DNA-Prot and outperformed DNAbinder and iDNA-Prot with performance improvement in the range of 3.97–9.52% in ACC and 0.08–0.19 in MCC. Furthermore, when the benchmark dataset was expanded with negative samples, the performance of enDNA-Prot outperformed the three existing methods by 2.83–16.63% in terms of ACC and 0.02–0.16 in terms of MCC. It indicated that enDNA-Prot is an effective method for DNA-binding protein identification and expanding training dataset with negative samples can improve its performance. For the convenience of the vast majority of experimental scientists, we developed a user-friendly web-server for enDNA-Prot which is freely accessible to the public.

  2. TGVizTab: An Ontology Visualisation Extension for Protégé

    OpenAIRE

    2003-01-01

    Ontologies are gaining a lot of interest and many are being developed to provide a variety of knowledge services. There is an increasing need for tools to graphically and in-teractively visualise such modelling structures to enhance their clarification, verification and analysis. Protégé 2000 is one of the most popular ontology modelling tools currently available. This paper introduces TGVizTab; a new Protégé plugin based on TouchGraph technology to graphically visualise Protégé?s ontologies....

  3. Combined heterozygosity of factor V leiden and the G20210A prothrombin gene mutation in a patient with cerebral cortical vein thrombosis.

    Science.gov (United States)

    Liu, X Y; Gabig, T G; Bang, N U

    2000-07-01

    Cerebral venous thrombosis (CVT) is a rare type of stroke with a variety of causes. Several reports have suggested that either factor V Leiden or G20210A prothrombin gene mutation is associated with an increased risk of CVT. The genetic thrombophilias are typically associated with other predisposing factors. We report a unique case of CVT in a patient with both the factor V Leiden and the G20210A prothrombin gene mutations without other identifiable precipitating factors in a 28-year-old white male in good health. MRI and cerebral arterial angiography showed cerebral cortical venous thrombosis. This case suggests that combined heterozygous individuals may be particularly prone to spontaneous thrombosis, like CVT.

  4. Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India.

    Science.gov (United States)

    Nishank, Sudhansu Sekhar; Singh, Mendi Prema Shyam Sunder; Yadav, Rajiv

    2013-11-01

    It is known that patients with sickle cell disease (SCD) present activation of the blood coagulation and fibrinolytic systems, especially during vaso-occlusive crises and also during the steady state of the disease. We determined whether the presence of the factor prothrombin gene G20210A variant, factor V gene G1691A mutation (factor V Leiden), and methylenetetrahydrofolate reductase (MTHFR) C677T polymorphisms may be risk factors for vascular complications in individuals with SCD. The study involved 150 patients with sickle cell anemia and 150 healthy controls of Central India. Genotyping of three thrombophilic mutations was carried out by PCR-RFLP methods using MnlI, Hind III, and Hinf I, respectively, for factor V Leiden, prothrombin, and MTHFR mutations. Patients with SCD had significantly higher prevalence of mutant variants of MTHFR gene (28.0% heterozygotes and 14.6% homozygotes) and FVL gene (14.6% heterozygotes) as compared to normal/control individuals, but complete absence of mutant variants of prothrombin gene. The patients with SCD having mutant variants of MTHFR and FVL genes showed higher incidence of pain in chest, abdomen, and bone joints along with early age of onset of clinical manifestations as well as frequent dependence on blood transfusion than those patients with SCD having wild variants of these thrombotic genes. As compared to control subjects, SCD individuals having mutant variants of FVL and MTHFR genes had significant association with higher levels of prothrombin fragment (F1+2), D-dimer, thrombin-antithrombin (TAT), and lower level of protein C. MTHFR C677T and FVL G1691A polymorphisms may be risk factors for increased vascular complications in patient with SCD. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  5. Increasing concentrations of prothrombin complex concentrate induce disseminated intravascular coagulation in a pig model of coagulopathy with blunt liver injury.

    Science.gov (United States)

    Grottke, Oliver; Braunschweig, Till; Spronk, Henri M H; Esch, Stephanie; Rieg, Annette D; van Oerle, Rene; ten Cate, Hugo; Fitzner, Christina; Tolba, Rene; Rossaint, Rolf

    2011-08-18

    Despite increasing use of prothrombin complex concentrate (PCC) to treat hemorrhage-associated coagulopathy, few studies have investigated PCC in trauma, and there is a particular lack of safety data. This study was performed to evaluate PCC therapy in a porcine model of coagulopathy with blunt liver injury. Coagulopathy was induced in 27 anesthetized pigs by replacing approximately 70% blood volume with hydroxyethyl starch 130/0.4 and Ringer's lactate solution; erythrocytes were collected and retransfused. Ten minutes after trauma, animals randomly received PCC (35 or 50 IU/kg) or saline. Coagulation parameters including thromboelastometry, thrombin generation, and blood loss were monitored for 2 hours. Internal organs were examined macroscopically and histologically to determine the presence of emboli and assess liver injury. Total blood loss was significantly lower and survival was higher in both PCC groups versus the control group (P < .05). These outcomes appeared to be dose-independent. Thromboembolism was found in all animals treated with 50 IU/kg PCC; 44% also showed signs of disseminated intravascular coagulation. Liver injury was similar in all animals. In conclusion, 35 IU/kg PCC safely improved coagulation and attenuated blood loss. However, the higher dose of PCC (50 IU/kg) appeared to increase the risk of thromboembolism and disseminated intravascular coagulation.

  6. Safety and efficacy of prothrombin complex concentrate as first-line treatment in bleeding after cardiac surgery.

    Science.gov (United States)

    Cappabianca, Giangiuseppe; Mariscalco, Giovanni; Biancari, Fausto; Maselli, Daniele; Papesso, Francesca; Cottini, Marzia; Crosta, Sandro; Banescu, Simona; Ahmed, Aamer B; Beghi, Cesare

    2016-01-06

    Bleeding after cardiac surgery requiring surgical reexploration and blood component transfusion is associated with increased morbidity and mortality. Although prothrombin complex concentrate (PCC) has been used satisfactorily in bleeding disorders, studies on its efficacy and safety after cardiopulmonary bypass are limited. Between January 2005 and December 2013, 3454 consecutive cardiac surgery patients were included in an observational study aimed at investigating the efficacy and safety of PCC as first-line coagulopathy treatment as a replacement for fresh frozen plasma (FFP). Starting in January 2012, PCC was introduced as solely first-line treatment for bleeding following cardiac surgery. After one-to-one propensity score-matched analysis, 225 pairs of patients receiving PCC (median dose 1500 IU) and FFP (median dose 2 U) were included. The use of PCC was associated with significantly decreased 24-h post-operative blood loss (836 ± 1226 vs. 935 ± 583 ml, p surgery setting, the use of PCC compared with FFP was associated with decreased post-operative blood loss and RBC transfusion requirements. However, PCC administration may be associated with a higher risk of post-operative AKI.

  7. 异常凝血酶原和肝细胞癌%Des-gamma-carboxy-prothrombin and Hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    袁联文; 唐伟; 幕内·雅敏

    2002-01-01

    目前肝细胞癌(hepatocellular carcinoma,HCC)的诊断主要有影像学诊断和血清肿瘤标志物的检测.异常凝血酶原(des-gamma-carboxy-prothrombin,DCP)又被称为PIVKA-Ⅱ(protein induced by vita-min K absence or antagonist-Ⅱ),与AFP(alpha-fetoprotein)和AFP-L3(alpha-fetoprotein L3 fraction)一样被认为是一种很有价值的肝细胞癌血清肿瘤标志物.在HCC的检测诊断上,它们之间无明显相关关系,而表现为一定的互补性,结合影像学诊断,动态观测HCC高危(肝炎、肝硬化)人群,这些血清肿瘤标志物有助于HCC的早期发现,同时对HCC的手术疗效的评价、预后的估评有着一定的指导意义.

  8. Factor V Leiden, Prothrombin and MTHFR Mutation in Patients with Preeclamsia, Intrauterine Growth Restriction and Placental Abruption.

    Science.gov (United States)

    Livrinova, Vesna; Lega, Marija Hadzi; Dimcheva, Anita Hristova; Samardziski, Igor; Isjanovska, Rozalinda

    2015-12-15

    Factor V Leiden, Prothrombin and MTHFR gene mutation, could have an influence in pregnancy with adverse outcome Preeclamsia, IUGR and Placental abruption. The aim of this study is to investigate the presence of above mentioned inherited thrombophilias and its statistical significance, distribution among the complicated and normal pregnancy, and relative risk for carrier of mutation to develop preeclampsia, IUGR and placental abruption. Prospective cohort study is implemented at University Clinic for Obstetric and Gynecology in Skopje, Republic of Macedonia. The study included 109 delivered patients: 40 with preeclapmsia, 22 with IUGR, 17 with placental abruption and 30 as control group with normal pregnancy. The amount of 3 ml venous blood has been used for detection of these point mutations using ThromboStrip -Opegen, QIAGEN kit manufactured for thrombotic risk. The highest frequency was found: in the group with preeclampsia 35% were MTHFR homozygous, IUGR -MTHFR heterozygous 45%, Placental abruption- 52.9% MTHFR heterozygous, and in the control group without thrombophilia 56.7%. There were combined thrombophilia in 3 patients. There aren`t statistical significance in presence of thrombophilia among groups (p > 0.05). Statistical significance (p Factor V Leiden heterozygous was 4.50 (0.47Factor V Leiden for placental abruption. Further investigations with more patients are warranted.

  9. Possible incorrect genotyping of heterozygous factor V Leiden and Prothrombin 20210 gene mutations by the GeneXpert assay.

    Science.gov (United States)

    Marturano, Alessandro; Bury, Loredana; Gresele, Paolo

    2014-08-05

    The GeneXpert analyzer is a hands-off system for the detection of Factor V Leiden and of Prothrombin G20210A (GPRO) gene thrombophilic mutations. Although the system is efficient and easy to use, we report the rare possibility of incorrect genotyping. 1648 samples were evaluated using the GeneXpert HemosIL Factor II and Factor V assay: 1319 were freshly analyzed while 329 were frozen, thawed and diluted with saline prior to analysis to avoid clogging of the instrument syringe. Two samples, both heterozygous, one for the factor V Leiden and the other for the GPRO gene, were incorrectly genotyped as homozygous for the relative mutation. Inspection of the Ct values and amplification curves and genotyping with PCR revealed the correct genotype as heterozygous for factor V Leiden and GPRO mutation. The GeneXpert HemosIL Factor II and Factor V assay is an automated, fast genotyping assay requiring almost no sample manipulation, advantageous characteristics if compared with other PCR-based methods. However, an inattentive use of it can generate incorrect diagnosis. A careful handling of the sample, in particular correct dilution of frozen/thawed samples before analysis, and the inspection of the amplification curves and Ct values are required to avoid artifacts. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Diagnostic performance of des-γ-carboxy prothrombin (DCP) for hepatocellular carcinoma: a bivariate meta-analysis.

    Science.gov (United States)

    Gao, P; Li, M; Tian, Q B; Liu, Dian-Wu

    2012-01-01

    Serum markers are needed to be developed to specifically diagnose Hepatocellular carcinoma (HCC). Des-γ-carboxy prothrombin (DCP) is a promising tool with limited expense and widely accessibility, but the reported results have been controversial. In order to review the performance of DCP for the diagnosis of HCC, the meta-analysis was performed. After a systematic review of relevant studies, the sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR, respectively) were pooled using a bivariate meta-analysis. Potential between-study heterogeneity was explored by meta-regression model. The post-test probability and the likelihood ratio scattergram to evaluate clinical usefulness were calculated. Based on literature review of 20 publications, the overall sensitivity, specificity, PLR and NLR of DCP for the detection of HCC were 67% (95%CI, 58%-74%), 92% (95%CI, 88%-94%), 7.9 (95%CI, 5.6-11.2) and 0.36 (95%CI, 0.29-0.46), respectively. The area under the bivariate summary receiving operating characteristics curve was 0.89 (95%CI, 0.85-0.92). Significant heterogeneity was present. In conclusion, the major role of DCP is the moderate confirmation of HCC. More prospective studies of DCP are needed in future.

  11. Plasma abnormal prothrombin (PIVKA-II): a new and reliable marker for the detection of hepatocellular carcinoma.

    Science.gov (United States)

    Takikawa, Y; Suzuki, K; Yamazaki, K; Goto, T; Madarame, T; Miura, Y; Yoshida, T; Kashiwabara, T; Sato, S

    1992-01-01

    We evaluated the clinical usefulness of a protein induced by vitamin K absence, antagonist-prothrombin (PIVKA-II), in detecting hepatocellular carcinoma (HCC) specifically in patients with liver cirrhosis, and the possible correlation between levels of PIVKA-II and pathological features of HCC. Plasma levels of PIVKA-II and alpha-fetoprotein (AFP) were measured in 628 patients with various diseases, including 253 with liver cirrhosis and 116 with HCC. PIVKA-II was detected (greater than or equal to 0.1 arbitrary unit/mL) in 54.3% of HCC and the concentration showed a positive correlation with the tumour size. As a screening test for the detection of HCC, PIVKA-II produced values comparable with those of AFP with a sensitivity, specificity and validity of 52.8, 98.8 and 51.6% respectively. Sixteen of 45 patients (37%) with HCC who had low AFP (less than 100 ng/mL) levels were positive for PIVKA-II. No apparent relationship, however, could be found between the levels of PIVKA-II and the aetiology or pathological findings of HCC. These results suggest that PIVKA-II can be a reliable marker for detecting HCC in patients with liver cirrhosis.

  12. Prothrombin complex concentrate administration for bleeding associated with non-vitamin K antagonist oral anticoagulants: The SAMURAI-NVAF study.

    Science.gov (United States)

    Yoshimura, Sohei; Sato, Shoichiro; Todo, Kenichi; Okada, Yasushi; Furui, Eisuke; Matsuki, Takayuki; Yamagami, Hiroshi; Koga, Masatoshi; Takahashi, Jun C; Nagatsuka, Kazuyuki; Arihiro, Shoji; Toyoda, Kazunori

    2017-04-15

    Antidotes appropriate for non-vitamin K antagonist (VKA) oral anticoagulants (NOACs) are not yet in widespread clinical use. Efficacy of prothrombin complex concentrate (PCC) in NOAC-associated bleeding remains unclarified. Ten NOAC users (4 women, median 74years old) who developed major bleeding and received PCC were prospectively enrolled. Eight single-center NOAC users (0 women, median 74years old) with intracerebral hemorrhage, who over the same period did not receive PCC, were studied for comparison. Of the 10 PCC-treated patients, 8 developed intracerebral hemorrhage, 1 developed subdural hematoma, and another developed gastrointestinal bleeding. The median size of intracerebral hemorrhage was 8mL, relatively lower than the reported size for patients without NOACs. Patients received a median of 1000IU or 16IU/kg of PCC. Before and 1h after PCC administration, the median PT-INR changed from 1.41 to 1.09 (pbleeding and were given relatively low doses of PCC. The effect of PCC on early cessation of bleeding was unclear, while the therapy did not trigger thromboembolic complications. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Influence of blood collection systems on the prothrombin time and international sensitivity index determined with human and rabbit thromboplastin reagents.

    Science.gov (United States)

    van den Besselaar, Anton M H P; Hoekstra, Martha M C L; Witteveen, Evelina; Didden, Jan H; van der Meer, Felix J M

    2007-05-01

    Three brands of blood collection tubes were studied for their influence on the prothrombin time (PT) and international sensitivity index (ISI) for 5 commercial thromboplastin reagents. With all reagents, PTs were shorter in Vacutainer (Becton Dickinson Vacutainer Systems, Plymouth, England) samples than in S-Monovette (Sarstedt, Nümbrecht, Germany) or Venosafe (Terumo Europe, Leuven, Belgium) samples. ISI values were higher with Vacutainer samples than with S-Monovette or Venosafe samples. The ISI differences between the tubes were small for Thromborel-S (2.1%; Dade Behring, Marburg, Germany) and Hepato Quick (1.1%; Diagnostica Stago, Asnières, France; Roche Diagnostics Nederland, Almere, the Netherlands) but greater for Neoplastin Plus (5.5%; Diagnostica Stago; Roche Diagnostics Nederland), Simplastin HTF (8.3%; bioMérieux, Durham, NC), and Innovin (8.8%; Dade Behring). The PT and ISI differences between the tubes could be explained mostly by the effect of magnesium ion contamination in the sodium citrate solutions. When PT ratios were transformed into international normalized ratios (INRs) using crossover ISI (ie, samples collected with one type of tube and ISI determined with another collection system for the PT reagent), the differences in mean INRs could be approximately 10%. For ISI calibration of reference thromboplastins, blood collection tubes should be used with minimal divalent metal ion contamination of the citrate solution.

  14. Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples

    Science.gov (United States)

    Casella, Stefania; Giannetto, Claudia; Giudice, Elisabetta

    2010-01-01

    The present study was to assess the effect of storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen concentration in blood samples of healthy dogs. Thirty-five dogs of various breeds were included in the study. Citrated blood samples were obtained and plasma was divided into four aliquots to assess selected clotting parameters by means of a coagulometer. The first aliquot was analysed within 1 h after collection, while the remaining 3 were stored at 8℃ for 4, 8 and 24 h, respectively. One-way repeated measures analysis of variance documented a significant decreasing effect on PT at 24 h compared to 8 h and on fibrinogen concentration after 8 and 24 h compared to sampling time and at 4 and 24 h compared to 8 h post sampling. In conclusion, the results of this study indicate that only fibrinogen appears prone to significant decrease. In fact, aPTT is not substantially affected by refrigeration for at least 24 h post sampling and PT showed a statistical difference that does not necessary indicate biological significance as the results obtained were within reference intervals for the dog. PMID:20458152

  15. An observational, prospective, two-cohort comparison of a fixed versus variable dosing strategy of prothrombin complex concentrate to counteract vitamin K antagonists in 240 bleeding emergencies.

    Science.gov (United States)

    Khorsand, Nakisa; Veeger, Nic J G M; van Hest, Reinier M; Ypma, Paula F; Heidt, Jeroen; Meijer, Karina

    2012-10-01

    Despite years of experience with vitamin K antagonist-associated bleeding events, there is still no evidence to help identify the optimal treatment with prothrombin complex concentrates. Variable dosing and fixed dose strategies are being used. In this observational prospective two-cohort study, we aimed to assess the non-inferiority of a low fixed PCC dose (1,040 IU Factor IX) compared to the registered variable dosing regimen based on baseline International Normalized Rate, bodyweight, and target International Normalized Rate, to counteract vitamin K antagonists in a bleeding emergency in a daily clinical practice setting. Non-inferiority of the fixed prothrombin complex concentrate dose was hypothesized with a margin of 4%. Main end points were proportion of patients reaching the target International Normalized Rate (complex concentrate treatment, and successful clinical outcome. Target International Normalized Rate was reached in 92% of the fixed dose patients (n=101) versus 95% of variable dose patients (n=139) resulting in a risk difference of -2.99% (90% CI: - 8.6 to 2.7) (non-inferiority not confirmed). Clinical outcome was successful in 96% and 88% of fixed versus variable dose, respectively, with a risk difference of 8.3% (90% CI: 2.7-13.9; non-inferiority confirmed). Although a lower fixed prothrombin complex concentrate dose was associated with successful clinical outcome, fewer patients reached the target International Normalized Rate.

  16. Concomitant homozygosity for the prothrombin gene variant with mild deficiency of antithrombin III in a patient with multiple hepatic infarctions: a case report

    Directory of Open Access Journals (Sweden)

    Macheta M

    2010-04-01

    Full Text Available Abstract Introduction Hereditary causes of visceral thrombosis or thrombosis should be sought among young patients. We present a case of a young man presenting with multiple hepatic infarctions resulting in portal hypertension due to homozygosity of the prothrombin gene mutation not previously described in literature. Case presentation A 42-year-old Caucasian man with a previous history of idiopathic deep vein thrombosis 11 years earlier presented with vague abdominal pains and mildly abnormal liver function tests. An ultrasound and computed tomography scan showed evidence of hepatic infarction and portal hypertension (splenic varices. A thrombophilia screen confirmed a homozygous mutation for the prothrombin gene mutation, with mildly reduced levels of anti-thrombin III (AT III. Subsequent testing of his father and brother revealed heterozygosity for the same gene mutation. Conclusion Hepatic infarction is unusual due to the rich dual arterial and venous blood supply to the liver. In the absence of an arterial or haemodynamic insult causing hepatic infarction, a thrombophilia should be considered. To our knowledge, this is the first reported case of a hepatic infarction due to homozygosity of the prothrombin gene mutation. It is unclear whether homozygotes have a higher risk of thrombosis than heterozygotes. In someone presenting with a first thrombosis with this mutation, the case for life-long anticoagulation is unclear, but it may be necessary to prevent a second and more severe second thrombotic event, as occurred in this case.

  17. The frequency of factor V Leiden and prothrombin G20210A mutations in Slovak and Roma (Gypsy) ethnic group of Eastern Slovakia.

    Science.gov (United States)

    Bôžiková, Alexandra; Gabriková, Dana; Sovičová, Adriana; Behulová, Regina; Mačeková, Soňa; Boroňová, Iveta; Petrejčíková, Eva; Soták, Miroslav; Bernasovská, Jarmila; Bernasovský, Ivan

    2012-10-01

    Factor V Leiden and prothrombin G20210A are the two most prevalent causes of inherited thrombophilia. The prevalence of these mutations varies widely in healthy Caucasian population. The aim of our study was to determine the frequency of factor V Leiden and prothrombin G20210A mutations in Slovak and Roma ethnic group from Eastern Slovakia. We analyzed 540 asymptomatic individuals (269 individuals of Slovak ethnicity and 271 individuals of Roma ethnicity) by real-time PCR method. The detected allele frequencies were 2.97 versus 6.64 % for factor V Leiden (p = 0.0049), and 0.74 versus 0.92 % for prothrombin mutation (p = 0.7463) in Slovak and Roma population, respectively. The Roma ethnic group had significantly higher prevalence of factor V Leiden mutation when compared to Slovak ethnic group. The allele frequency of factor V Leiden in ethnic Romanies from Eastern Slovakia was one of the highest in Europe. Our results confirm an uneven geographical and ethnic distribution of factor V Leiden.

  18. Elevated levels of antibodies against phosphatidylserine/prothrombin complex and/or cardiolipin associated with infection and recurrent purpura in a child: a forme fruste of antiphospholipid syndrome?

    Science.gov (United States)

    Kinoshita, Yuri; Mayumi, Nobuko; Inaba, Motoyuki; Igarashi, Touru; Katagiri, Ichigen; Kawana, Seiji

    2015-07-15

    Antiphospholipid syndrome is an autoimmune disorder characterized by the occurrence of venous and arterial thrombosis, as well as morbidity in pregnancy, in the presence of anti-phospholipid antibodies. The diagnosis of antiphospholipid syndrome is usually established based on clinical and laboratory findings by strictly following the 2006 Sapporo classification. However, the diagnosis remains challenging owing to the ongoing debates on the serological criteria. We report a case we describe as forme fruste antiphospholipid syndrome in which these criteria were not fulfilled. Purpura appeared repeatedly in a female infant starting from the age of 6 months and following episodes of upper respiratory infections and vaccinations. The levels of anti-cardiolipin IgG antibodies and anti-phosphatidylserine/prothrombin complex antibodies were elevated in accordance with these events. Histopathological evaluation revealed multiple small vessel thrombi in the dermis and adipose tissue. After 2 weeks of treatment with aspirin and heparin, the cutaneous symptoms subsided. Infection has long been associated with antiphospholipid syndrome, and anti-phosphatidylserine/prothrombin antibodies are considered a new marker for the diagnosis of antiphospholipid syndrome. Forme fruste antiphospholipid syndrome should be considered even if the antiphospholipid syndrome diagnostic criteria are not completely fulfilled, especially in the presence of elevated levels of anti-phosphatidylserine/prothrombin antibodies and known preceding infections.

  19. EFFECT OF METHANOLIC SEED EXTRACT OF PERSEA AMERICANA(AVOCADO PEAR ON PROTHROMBIN TIME AND ACTIVATED PARTIAL THROMBOPLASTIN TIME IN MICE

    Directory of Open Access Journals (Sweden)

    2016-11-01

    Full Text Available Twenty (20 adult albino mice were used in the study to determine the effect of methanolic seed extract of Persea Americana on prothrombin time (PT and activated partial thromboplastin time (APTT test. The mice were obtained and kept for 2 weeks to acclimatize. They were weighed and divided into 5 groups. Group A served as control without the extract. Groups B to E were orally administered with graded doses of 200mg, 400 mg, 800 mg and 1600mg/kg body weight per mice daily for 28 days. Blood samples were collected through the median canthus into ti-sodium citrate anticoagulant containers for the analysis of PT and APTT, using standard operative procedure. The analysis was carried out at the Haematology Laboratory of University of Nigeria Teaching Hospital (UNTH Enugu. The results showed a prolonged APTT time at all the doses of the extract when compared with the control (P and lt;0.05. The prothrombin time at the dosage of 200mg/kg did not differ when compared with the control (P and gt;0.05. The increase in PT and APTT was dose dependent. This result pattern suggests that the extract causes prolonged prothrombin time and APTT at various concentrations possibly due to its high potassium content. The extract can be recommended in anticoagulant therapy since it prolongs PT and APTT.

  20. Interactivity between protégés and scientists in an electronic mentoring program

    DEFF Research Database (Denmark)

    Bonnett, Cara; Wildemuth, Barbara M.; Sonnenwald, Diane H.

    2006-01-01

    Interactivity is defined by Henri (1992) as a three-step process involving communication of information, a response to this information, and a reply to that first response. It is a key dimension of computer-mediated communication, particularly in the one-on-one communication involved......-protégé pairs rated as effective by both mentors and protégés posted more messages overall, had well-structured threads, had protégé and mentor postings that were similar in topic coverage and message length, and had little overt "management" behavior by mentors. However, there appears to be no clear recipe...... for successful interaction. Not only are there a variety of factors at play in developing an online relationship in this context, but mentor-protégé pairs can falter at various stages in the process and in various ways. © Springer 2006....

  1. RaftProt: mammalian lipid raft proteome database.

    Science.gov (United States)

    Shah, Anup; Chen, David; Boda, Akash R; Foster, Leonard J; Davis, Melissa J; Hill, Michelle M

    2015-01-01

    RaftProt (http://lipid-raft-database.di.uq.edu.au/) is a database of mammalian lipid raft-associated proteins as reported in high-throughput mass spectrometry studies. Lipid rafts are specialized membrane microdomains enriched in cholesterol and sphingolipids thought to act as dynamic signalling and sorting platforms. Given their fundamental roles in cellular regulation, there is a plethora of information on the size, composition and regulation of these membrane microdomains, including a large number of proteomics studies. To facilitate the mining and analysis of published lipid raft proteomics studies, we have developed a searchable database RaftProt. In addition to browsing the studies, performing basic queries by protein and gene names, searching experiments by cell, tissue and organisms; we have implemented several advanced features to facilitate data mining. To address the issue of potential bias due to biochemical preparation procedures used, we have captured the lipid raft preparation methods and implemented advanced search option for methodology and sample treatment conditions, such as cholesterol depletion. Furthermore, we have identified a list of high confidence proteins, and enabled searching only from this list of likely bona fide lipid raft proteins. Given the apparent biological importance of lipid raft and their associated proteins, this database would constitute a key resource for the scientific community.

  2. Finantssektori protsüklilisus : olemus, avaldumine ja mõju tasandamine / Kadri Männasoo

    Index Scriptorium Estoniae

    Männasoo, Kadri

    2003-01-01

    Ilmunud ka: Kroon & Economy nr. 2, lk. 32-47. Autor vaatleb protsüklilisuse olemust ja avaldumisvorme. Samas käsitleb ta protsüklilisuse vastu võitlemise meetmeid, tuues esile neid, mida on teoorias välja pakutud ning püütud Eesti oludele kohandada. Ta esitab järeldused ja ettepanekud, mille rakendamine võiks olla abiks Eesti finantsstabiilsuse suurendamisel. Tabelid ja diagrammid

  3. Motivating protégés' personal learning in teams: a multilevel investigation of autonomy support and autonomy orientation.

    Science.gov (United States)

    Liu, Dong; Fu, Ping-Ping

    2011-11-01

    This study examined the roles of 3 multilevel motivational predictors in protégés' personal learning in teams: an autonomy-supportive team climate, mentors' autonomy support, and protégés' autonomy orientation. The authors followed 305 protégés in 58 teams for 12 weeks and found that all 3 predictors were positively related to the protégés' personal learning in teams and that an autonomy-supportive team climate augmented the effects of mentors' autonomy support and protégés' autonomy orientation on protégés' personal learning in teams. Protégés' personal learning in teams mediated the interactive effects of an autonomy-supportive team climate with mentors' autonomy support or protégés' autonomy orientation on protégés' behavioral and attitudinal outcomes, including their organizational citizenship behaviors and job involvement. The findings of this study provide business researchers and practitioners with valuable insights into the management of autonomy.

  4. 49 CFR 26.35 - What role do business development and mentor-protégé programs have in the DBE program?

    Science.gov (United States)

    2010-10-01

    ... participate in the mentor-protégé program. (2) During the course of the mentor-protégé relationship, you must... 49 Transportation 1 2010-10-01 2010-10-01 false What role do business development and mentor-protÃ... What role do business development and mentor-protégé programs have in the DBE program? (a) You may...

  5. Seleção de protótipos: combinando auto-geração de protótipos e mistura de gaussianas

    OpenAIRE

    de Santana Pereira, Cristiano

    2008-01-01

    Seleção de protótipos é uma técnica de aprendizagem de máquina cujo objetivo é a escolha ou produção de instâncias de dados que consigam a melhor representação para os dados do problema realçando as fronteiras de decisão e mantendo a separação entre as classes. A idéia é reduzir a quantidade de dados e ainda assim obter um conjunto de protótipos que minimize o erro de classificação. As estratégias baseadas em protótipos têm sido bastante utilizadas em aplicações reais nos mais diversos domíni...

  6. Factor V Leiden, prothrombin G20210A, and methylene tetrahydrofolate reductase mutations and stillbirth: the Stillbirth Collaborative Research Network.

    Science.gov (United States)

    Silver, Robert M; Saade, George R; Thorsten, Vanessa; Parker, Corette B; Reddy, Uma M; Drews-Botsch, Carey; Conway, Deborah; Coustan, Donald; Dudley, Donald J; Bukowski, Radek; Rowland Hogue, Carol J; Pinar, Halit; Varner, Michael W; Goldenberg, Robert; Willinger, Marian

    2016-10-01

    An evaluation for heritable thrombophilias is recommended in the evaluation of stillbirth. However, the association between thrombophilias and stillbirth remains uncertain. We sought to assess the association between maternal and fetal/placental heritable thrombophilias and stillbirth in a population-based, case-control study in a geographically, racially, and ethnically diverse population. We conducted secondary analysis of data from the Stillbirth Collaborative Research Network, a population-based case-control study of stillbirth. Testing for factor V Leiden, prothrombin G20210A, methylene tetrahydrofolate reductase C677T and A1298C, and plasminogen activating inhibitor (PAI)-1 4G/5G mutations was done on maternal and fetal (or placental) DNA from singleton pregnancies. Data analyses were weighted for oversampling and other aspects of the design. Odds ratios (OR) were generated from univariate models regressing stillbirth/live birth status on each thrombophilia marker. Results were available for ≥1 marker in 488 stillbirths and 1342 live birth mothers and 405 stillbirths and 990 live birth fetuses. There was an increased odds of stillbirth for maternal homozygous factor V Leiden mutation (2/488; 0.4% vs 1/1380; 0.0046%; OR, 87.44; 95% confidence interval, 7.88-970.92). However, there were no significant differences in the odds of stillbirth for any other maternal thrombophilia, even after stratified analyses. Fetal 4G/4G PAI-1 (OR, 0.63; 95% confidence interval, 0.43-0.91) was associated with decreased odds of stillbirth. Other fetal thrombophilias were similar among groups. Most maternal and fetal thrombophilias were not associated with stillbirth. Maternal factor V Leiden was weakly associated with stillbirth, and the fetal PAI-1 4G/4G polymorphism was associated with live birth. Our data do not support routine testing for heritable thrombophilias as part of an evaluation for possible causes of stillbirth. Copyright © 2016. Published by Elsevier Inc.

  7. Plasminogen activator inhibitor-1 4G/5G polymorphism, factor V Leiden, prothrombin mutations and the risk of VTE recurrence.

    Science.gov (United States)

    Sundquist, Kristina; Wang, Xiao; Svensson, Peter J; Sundquist, Jan; Hedelius, Anna; Larsson Lönn, Sara; Zöller, Bengt; Memon, Ashfaque A

    2015-11-25

    Plasminogen-activator inhibitor (PAI)-1 is an important inhibitor of the plasminogen/plasmin system. PAI-1 levels are influenced by the 4G/5G polymorphism in the PAI-1 promoter. We investigated the relationship between the PAI-1 polymorphism and VTE recurrence, and its possible modification by factor V Leiden (FVL) and prothrombin (PTM) mutations. Patients (n=1,069) from the Malmö Thrombophilia Study were followed from discontinuation of anticoagulant treatment until diagnosis of VTE recurrence or the end of the study (maximum follow-up 9.8 years). One hundred twenty-seven patients (11.9 %) had VTE recurrence. PAI-1 was genotyped by TaqMan PCR. Cox regression analysis adjusted for age, sex and acquired risk factors of VTE showed no evidence of an association between PAI-1 genotype and risk of VTE recurrence in the study population as a whole. However, by including an interaction term in the analysis we showed that FVL but not PTM modified the effect of PAI-1 genotype: patients with the 4G allele plus FVL had a higher risk of VTE recurrence [hazard ratio (HR) =2.3, 95 % confidence interval (CI) =1.5-3.3] compared to patients with the 4G allele but no FVL (reference group) or FVL irrespective of PAI-1 genotype (HR=1.8, 95 % CI=1.3-2.5). Compared to reference group, 5G allele irrespective of FVL was associated with lower risk of VTE recurrence only when compared with 4G allele together with FVL. In conclusion, FVL has a modifying effect on PAI-1 polymorphism in relation to risk of VTE recurrence. The role of PAI-1 polymorphism as a risk factor of recurrent VTE may be FVL dependent.

  8. ChemProt: a disease chemical biology database

    DEFF Research Database (Denmark)

    Taboureau, Olivier; Nielsen, Sonny Kim; Audouze, Karine Marie Laure

    2011-01-01

    Systems pharmacology is an emergent area that studies drug action across multiple scales of complexity, from molecular and cellular to tissue and organism levels. There is a critical need to develop network-based approaches to integrate the growing body of chemical biology knowledge with network...... biology. Here, we report ChemProt, a disease chemical biology database, which is based on a compilation of multiple chemical-protein annotation resources, as well as disease-associated protein-protein interactions (PPIs). We assembled more than 700 000 unique chemicals with biological annotation for 30...... evaluation of environmental chemicals, natural products and approved drugs, as well as the selection of new compounds based on their activity profile against most known biological targets, including those related to adverse drug events. Results from the disease chemical biology database associate citalopram...

  9. Does point of care prothrombin time measurement reduce the transfusion of fresh frozen plasma in patients undergoing major surgery? The POC-OP randomized-controlled trial

    Directory of Open Access Journals (Sweden)

    Alberio Lorenzo

    2009-11-01

    Full Text Available Abstract Background Bleeding is a frequent complication during surgery. The intraoperative administration of blood products, including packed red blood cells, platelets and fresh frozen plasma (FFP, is often live saving. Complications of blood transfusions contribute considerably to perioperative costs and blood product resources are limited. Consequently, strategies to optimize the decision to transfuse are needed. Bleeding during surgery is a dynamic process and may result in major blood loss and coagulopathy due to dilution and consumption. The indication for transfusion should be based on reliable coagulation studies. While hemoglobin levels and platelet counts are available within 15 minutes, standard coagulation studies require one hour. Therefore, the decision to administer FFP has to be made in the absence of any data. Point of care testing of prothrombin time ensures that one major parameter of coagulation is available in the operation theatre within minutes. It is fast, easy to perform, inexpensive and may enable physicians to rationally determine the need for FFP. Methods/Design The objective of the POC-OP trial is to determine the effectiveness of point of care prothrombin time testing to reduce the administration of FFP. It is a patient and assessor blind, single center randomized controlled parallel group trial in 220 patients aged between 18 and 90 years undergoing major surgery (any type, except cardiac surgery and liver transplantation with an estimated blood loss during surgery exceeding 20% of the calculated total blood volume or a requirement of FFP according to the judgment of the physicians in charge. Patients are randomized to usual care plus point of care prothrombin time testing or usual care alone without point of care testing. The primary outcome is the relative risk to receive any FFP perioperatively. The inclusion of 110 patients per group will yield more than 80% power to detect a clinically relevant relative risk

  10. A review of the clinical utility of INR to monitor and guide administration of prothrombin complex concentrate to orally anticoagulated patients

    DEFF Research Database (Denmark)

    Sølbeck, Sacha; Ostrowski, Sisse R; Johansson, Pär I

    2012-01-01

    the initiation steps of the haemostatic process. The objective of the present review was to reassess the evidence for using INR as a tool to guide administration of prothrombin complex concentrates (PCC) to OAC patients. A Medline and Cochrane database search was conducted using the following keywords...... evidence for the usefulness of INR as a tool to monitor and guide reversal of OAC induced coagulopathy in patients with PCC, which is expected given that it is a plasma-based assay only reflecting a limited part of the haemostatic process....

  11. Emergency management of major bleeding in a case of maxillofacial trauma and anticoagulation: utility of prothrombin complex concentrates in the shock room

    Directory of Open Access Journals (Sweden)

    Alessandro Morotti

    2015-03-01

    Full Text Available Life-threatening bleeding in anticoagulation with Warfarin is an emergency challenging issue. Several approaches are available to treat bleeding in either over-anticoagulation or propeanticoagulation, including vitamin K, fresh frozen plasma and prothrombin complex concentrates (PCC administration. In coexisting trauma-induced bleeding and anticoagulation, reversal of anticoagulation must be a rapid and highly effective procedure. Furthermore the appropriate treatment must be directly available in each shock rooms to guarantee the rapid management of the emergency. PCC require a simple storage, rapid accessibility, fast administration procedures and high effectiveness. Here we report the utility of PCC in management of a craniofacial trauma in proper-anticoagulation.

  12. [Evaluation of a new portable device for measuring prothrombin time-internatinal normalized ratio; comparison between the conventional method and the new CoagCheck XS].

    Science.gov (United States)

    Yamamoto, K; Hashimoto, K; Sakamoto, Y; Okuyama, H; Kawada, N; Inoue, T

    2008-07-01

    We evaluated a new portable device (CoaguCheck XS) for measuring the prothrombin time-international normalized ratio (PT-INR) in 63 patients after cardiac surgery, and compared the results with those of the conventional method. There was a good correlation between the PT-INR values measured conventionally and those obtained with the CoaguCheck XS. This new device was easy to use, data were obtained rapidly, and the results were reliable. The CoaguCheck XS will be particularly useful for outpatients. PT-INR self-management is expected to be introduced as soon as health insurance coverage is obtained.

  13. A case of catastrophic antiphospholipid antibody syndrome complicated with systemic lupus erythematosus, double positive for anti-cardiolipin/β₂ glycoprotein I and anti-phosphatidylserine/prothrombin autoantibodies.

    Science.gov (United States)

    Hirakawa, Eri; Saito, Kazuyoshi; Hirata, Shintaro; Atsumi, Tatsuya; Koike, Takao; Tanaka, Yoshiya

    2012-09-01

    A 16-year-old male with severe thrombocytopenia and progressive multiple organ infarctions was diagnosed as having catastrophic antiphospholipid syndrome (CAPS) complicated with systemic lupus erythematosus, and was successfully treated with combination of anticoagulants, corticosteroids, plasma exchange, and intravenous cyclophosphamide. Antibodies to phosphatidylserine/prothrombin (PS/PT) complex and cardiolipin (CL)/β(2)-glycoprotein I (β(2)GPI) were simultaneously detected, indicating that the different pathways of both PS/PT and CL/β(2)GPI might be associated with the radical manifestation of CAPS.

  14. Prothrombin G20210A mutation is associated with young-onset stroke: the genetics of early-onset stroke study and meta-analysis.

    Science.gov (United States)

    Jiang, Baijia; Ryan, Kathleen A; Hamedani, Ali; Cheng, Yuching; Sparks, Mary J; Koontz, Deborah; Bean, Christopher J; Gallagher, Margaret; Hooper, W Craig; McArdle, Patrick F; O'Connell, Jeffrey R; Stine, O Colin; Wozniak, Marcella A; Stern, Barney J; Mitchell, Braxton D; Kittner, Steven J; Cole, John W

    2014-04-01

    Although the prothrombin G20210A mutation has been implicated as a risk factor for venous thrombosis, its role in arterial ischemic stroke is unclear, particularly among young adults. To address this issue, we examined the association between prothrombin G20210A and ischemic stroke in a white case-control population and additionally performed a meta-analysis. From the population-based Genetics of Early Onset Stroke (GEOS) study, we identified 397 individuals of European ancestry aged 15 to 49 years with first-ever ischemic stroke and 426 matched controls. Logistic regression was used to calculate odds ratios (ORs) in the entire population and for subgroups stratified by sex, age, oral contraceptive use, migraine, and smoking status. A meta-analysis of 17 case-control studies (n=2305 cases <55 years) was also performed with and without GEOS data. Within GEOS, the association of the prothrombin G20210A mutation with ischemic stroke did not achieve statistical significance (OR=2.5; 95% confidence interval [CI]=0.9-6.5; P=0.07). However, among adults aged 15 to 42 years (younger than median age), cases were significantly more likely than controls to have the mutation (OR=5.9; 95% CI=1.2-28.1; P=0.03), whereas adults aged 42 to 49 years were not (OR=1.4; 95% CI=0.4-5.1; P=0.94). In our meta-analysis, the mutation was associated with significantly increased stroke risk in adults ≤55 years (OR=1.4; 95% CI=1.1-1.9; P=0.02), with significance increasing with addition of the GEOS results (OR=1.5; 95% CI=1.1-2.0; P=0.005). The prothrombin G20210A mutation is associated with ischemic stroke in young adults and may have an even stronger association among those with earlier onset strokes. Our finding of a stronger association in the younger young adult population requires replication.

  15. Plasma lipids and prothrombin time in rats fed palm oil and other commonly used fats in Egypt

    Directory of Open Access Journals (Sweden)

    Hussein, Mona M.

    1993-02-01

    Full Text Available Sprague-Dawley rats were fed for a total period of 8 weeks on six diets that were different in the source of their fat content. The fat content was provided either, palm oil or palm olein or corn oil or hydrogenated fat, or frying palm oil and mixture of corn oil + hydrogenated fat in the ratio (1:1. The latter was given to the control group. Animals fed these various experimental diets showed statistically significant differences in serum cholesterol and serum triglycerides content among all group. Increased HDL-cholesterol content was evident in animals fed on palm-olein and palm oil. The frying oil fed group showed lowest HDL-cholesterol content. In these experiments palm olein fed animals showed highest ratio of HDL-cholesterol to total cholesterol while the lowest ratio was shown in rats fed on frying oil. Prothrombin (PT and activated partial thromboplastin time (PTT showed higher values In palm oil, palm olein and corn oil diets as compared to all groups with each other.

    Ratas Sprague-Dawley fueron alimentadas durante un periodo total de 8 semanas con seis dietas diferentes en su contenido graso. El contenido graso fue proporcionado por aceite de palma u oleína de palma o aceite de maíz o grasa hidrogenada o aceite de palma de fritura y mezcla de aceite de maíz + grasa hidrogenada en la relación (1:1. El último fue dado al grupo de control. Los animales alimentados con las diferentes dietas experimentales mostraron diferencias significativas estadísticamente en el contenido en colesterol y triglicéridos en suero entre todos los grupos. El aumento en contenido HDL-colesterol fue evidente en animales alimentados con oleína de palma y aceite de palma. El grupo alimentado con aceite de fritura mostró el más bajo contenido en HDL-colesterol. En estos experimentos, los animales alimentados con oleína de palma mostraron la mayor relación de HDL-colesterol a colesterol total, mientras que la relación más baja fue mostrada

  16. The Frequency of Factor V Leiden, Prothrombin G20210A and Methylenetetrahydrofolate Reductase C677T Mutations in Migraine Patients

    Directory of Open Access Journals (Sweden)

    Ruhsen Öcal

    2010-12-01

    Full Text Available OBJECTIVE: Migraine is an independent risk factor for ischemic stroke, but its pathophysiology is still unclear. Genetic factors that predispose patients to thrombosis have been studied in patients with migraine to highlight the pathogenesis, but the results remain controversial. In this study, the frequencies of factor V Leiden (FVL, prothrombin (Pt G20210A and methylenetetrahydrofolate reductase (MTHFR C677T mutations were investigated. METHODS: One hundred and sixty patients aged of 15 to 55 years with no history of systemic disease and who had been diagnosed as migraine according to the International Headache Society (IHS diagnostic criteria at Baskent University Hospital Neurology Outpatient Clinics were investigated for FVL, Pt G20210A and MTHFR C677T mutations from their genomic DNA, and the results were compared with those of healthy controls. RESULTS: One hundred and fifty five (96.9% of 160 migraine patients were homozygote normal, 5 (3.1% were heterozygote and none of them were homozygote mutant for FVL. The control group had 9.8% heterozygote individuals but the difference between the percentages was not statistically significant (p> 0.05. There were no homozygote mutant individuals in the Turkish population study in normal subjects like our study. Thirty nine (24.4% of 160 migraine patients were heterozygote and 8 (5% were homozygote mutant for MTHFR C677T. The control group had 37 (34.9% heterozygote and 6 (5.6% homozygote mutant individuals. The difference between the percentages was not statistically significant (p= 0.15. Three (1.9% of 160 migraine patients were heterozygote and 5 (2.9% of the control group were heterozygote mutant for Pt G20210A mutation. The control group had 37 (34.9% heterozygote and 6 (5.6% homozygote mutant individuals. The difference between the percentages was not statistically significant (p= 0.420. CONCLUSION: Our study indicates that FVL, Pt G20210A and MTHFR C677T gene mutations, which are considered

  17. Prothrombin Time and Activated Partial Thromboplastin Time Testing: A Comparative Effectiveness Study in a Million-Patient Sample.

    Directory of Open Access Journals (Sweden)

    Manu N Capoor

    Full Text Available A substantial fraction of all American healthcare expenditures are potentially wasted, and practices that are not evidence-based could contribute to such waste. We sought to characterize whether Prothrombin Time (PT and activated Partial Thromboplastin Time (aPTT tests of preoperative patients are used in a way unsupported by evidence and potentially wasteful.We evaluated prospectively-collected patient data from 19 major teaching hospitals and 8 hospital-affiliated surgical centers in 7 states (Delaware, Florida, Maryland, Massachusetts, New Jersey, New York, Pennsylvania and the District of Columbia. A total of 1,053,472 consecutive patients represented every patient admitted for elective surgery from 2009 to 2012 at all 27 settings. A subset of 682,049 patients (64.7% had one or both tests done and history and physical (H&P records available for analysis. Unnecessary tests for bleeding risk were defined as: PT tests done on patients with no history of abnormal bleeding, warfarin therapy, vitamin K-dependent clotting factor deficiency, or liver disease; or aPTT tests done on patients with no history of heparin treatment, hemophilia, lupus anticoagulant antibodies, or von Willebrand disease. We assessed the proportion of patients who received PT or aPTT tests who lacked evidence-based reasons for testing.This study sought to bring the availability of big data together with applied comparative effectiveness research. Among preoperative patients, 26.2% received PT tests, and 94.3% of tests were unnecessary, given the absence of findings on H&P. Similarly, 23.3% of preoperative patients received aPTT tests, of which 99.9% were unnecessary. Among patients with no H&P findings suggestive of bleeding risk, 6.6% of PT tests and 7.1% of aPTT tests were either a false positive or a true positive (i.e. indicative of a previously-undiagnosed potential bleeding risk. Both PT and aPTT, designed as diagnostic tests, are apparently used as screening tests

  18. Mentoring early-career preventionists: current views from mentors and protégés.

    Science.gov (United States)

    Véronneau, Marie-Hélène; Cance, Jessica Duncan; Ridenour, Ty A

    2012-10-01

    In prevention science, much of the training occurs outside of a formal graduate program and mentorship is invaluable to early-career individuals. A sample of the Society for Prevention Research (SPR) membership (N = 97) from a wide range of career levels completed an online questionnaire in spring 2010. Almost 20% identified as mentors, 32% as protégés, and 49% as both a mentor and a protégé. Most mentoring relationships were established in graduate school, but professional organizations such as SPR facilitated nearly one in five mentoring relationships. Qualitative results suggested that participants value their professional organization's support of mentoring and would support initiatives to increase mentoring relationships specifically among SPR members. Although all mentor functions and protégé responsibilities were rated as important, professional support was the highest ranked mentor function and taking initiative the highest ranked protégé responsibility. Additionally, the qualitative results revealed that interpersonal skills and commitment to the mentoring process were seen as key to positive mentoring relationships. We also found that formal documentation of mentoring agreements was rare and a slight preference for a match on gender or ethnicity was observed for protégés from nondominant groups. The discussion includes implications for individuals and implications for promoting high-quality mentoring within professional organizations.

  19. Prot-2S: a new python web tool for protein secondary structure studies.

    Science.gov (United States)

    Munteanu, Cristian R; Magalhães, Alexandre L

    2009-01-01

    Prot-2S is a bioinformatics web application devised to analyse the protein chain secondary structures (2S) (http:/ /www.requimte.pt:8080/Prot-2S/). The tool is built on the RCSB Protein Data Bank PDB and DSSP application/files and includes calculation/graphical display of amino acid propensities in 2S motifs based on any user amino acid classification/code (for any particular protein chain list). The interface can calculate the 2S composition, display the 2S subsequences and search for DSSP non-standard residues and for pairs/triplets/quadruplets (amino acid patterns in 2S motifs). This work presents some Prot-2S applications showing its usefulness in protein research and as an e-learning tool as well.

  20. Lack of association between factor V Leiden and prothrombin G20210A polymorphisms in Tunisian subjects with a history of myocardial infarction.

    Science.gov (United States)

    Berredjeb Ben Slama, Dhouha; Fekih-Mrissa, Najiba; Haggui, Abdeddayem; Nsiri, Brahim; Baraket, Nadia; Haouala, Habib; Gritli, Nasreddine

    2013-01-01

    Myocardial infarction is a multifactorial disease. It is provoked by occlusions in the coronary arteries resulting from exposure to multiple risk factors. To study the risk of myocardial infarction associated with the gene polymorphisms of factor V Leiden and factor II (G20210A). Cases consisted of 100 myocardial infarction patients who were hospitalized in the Principal Military Hospital of Tunis and 200 control subjects with no history of myocardial infarction. The prevalence of the factor V Leiden was higher in myocardial infarction patients (9%) than in control subjects (6%) with an OR=1.55 (95% CI=0.58-4.12), whereas the prevalence of prothrombin G20210A mutation was 3% and 2.5% in the patient and control groups, respectively [OR=1.21 (95% CI=0.22-5.94)]. Our results indicate that neither factor V Leiden nor the prothrombin G20210A contributed to the risk factors for myocardial infarction. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. Association of factor V Leiden, Janus kinase 2, prothrombin, and MTHFR mutations with primary Budd-Chiari syndrome in Egyptian patients.

    Science.gov (United States)

    El Sebay, Hatem M; Safan, Manal A; Daoud, Ashraf A; Tayel, Safaa I; Nouh, Mohamed A; El Shafie, Shymaa

    2016-01-01

    Budd-Chiari syndrome (BCS) is defined as obstruction of hepatic venous outflow anywhere from the small hepatic veins to the suprahepatic inferior vena cava. The pathogenesis of BCS is still not fully understood. This study aimed to evaluate the association of factor V Leiden (FVL), Janus kinase 2 (JAK2), prothrombin, and methylene tetrahydrofolate reductase (MTHFR) mutations with primary BCS. The study was carried out on 35 patients with primary BCS and 15 age and gender matched healthy individuals as a control group. Genotyping of FVL, prothrombin, and MTHFR mutations was determined by GENEQUALITY AB-THROMBO TYPE kit based on the reverse hybridization principle. JAK2 mutation was determined by polymerase chain reaction-restriction fragment length polymorphism. There was a statistically significant difference between patients and controls regarding FVL, MTHFR C677T, and MTHFR A1298C mutations with odds ratio of 1.83, 2.0, and 1.79, respectively. Hetero MTHFR C677T, hetero FVL, and hetero MTHFR A1298C were the most common etiological factors being responsible for 57.1, 42.9, and 42.9% of primary BCS cases, respectively. It could be concluded that BCS is a multifactorial disease; in the current study, MTHFR C677T mutation was the most common cause of disease. Identification of one cause of BCS should not eliminate investigations for detection of other etiological factors. © 2015 Journal of Gastroenterology and Hepatology Foundation and Wiley Publishing Asia Pty Ltd.

  2. Desnutrição protéico-energética no paciente gastrectomizado

    OpenAIRE

    PAPINI-BERTO,Silvia Justina; Maio,Regiane; Módolo, Ana Karia; SANTOS,Maria Dorotéia Borges dos; Dichi, Isaias; Burini, Roberto Carlos [UNESP

    2002-01-01

    Racional - A gastrectomia traz conseqüências nutricionais inevitáveis mas atenuáveis, dependendo da dietoterapia pós-operatória. Embora observada, essa desnutrição protéico-energética é pouco dimensionada, provavelmente, pela falta de consenso metodológico. Objetivo - Avaliar o grau de desnutrição protéico-energética do paciente gastrectomizado, utilizando-se de indicadores isolados ou combinados. Pacientes e Métodos - Foram estudados 71 pacientes com gastrectomia parcial (n = 53) ou total (n...

  3. gDNA-Prot: Predict DNA-binding proteins by employing support vector machine and a novel numerical characterization of protein sequence.

    Science.gov (United States)

    Zhang, Yan-Ping; Wuyunqiqige; Zheng, Wei; Liu, Shuyi; Zhao, Chunguang

    2016-10-01

    DNA-binding proteins are the functional proteins in cells, which play an important role in various essential biological activities. An effective and fast computational method gDNA-Prot is proposed to predict DNA-binding proteins in this paper, which is a DNA-binding predictor that combines the support vector machine classifier and a novel kind of feature called graphical representation. The DNA-binding protein sequence information was described with the 20 probabilities of amino acids and the 23 new numerical graphical representation features of a protein sequence, based on 23 physicochemical properties of 20 amino acids. The Principal Components Analysis (PCA) was employed as feature selection method for removing the irrelevant features and reducing redundant features. The Sigmod function and Min-max normalization methods for PCA were applied to accelerate the training speed and obtain higher accuracy. Experiments demonstrated that the Principal Components Analysis with Sigmod function generated the best performance. The gDNA-Prot method was also compared with the DNAbinder, iDNA-Prot and DNA-Prot. The results suggested that gDNA-Prot outperformed the DNAbinder and iDNA-Prot. Although the DNA-Prot outperformed gDNA-Prot, gDNA-Prot was faster and convenient to predict the DNA-binding proteins. Additionally, the proposed gNDA-Prot method is available at http://sourceforge.net/projects/gdnaprot.

  4. 48 CFR 719.273-4 - Eligibility of Mentor and Protégé firms.

    Science.gov (United States)

    2010-10-01

    ... INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS The U.S. Agency for International... business entities approved as Mentors may enter into agreements (hereafter referred to as “Mentor-Protégé... subcontractors. Eligible small business entities capable of providing developmental assistance may be approved...

  5. 48 CFR 719.273-5 - Selection of Protégé firms.

    Science.gov (United States)

    2010-10-01

    ...© firms. 719.273-5 Section 719.273-5 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS The U.S. Agency for International Development... responsible for selecting Protégé firms. Mentors are encouraged to select from a broad base of small...

  6. 48 CFR 1019.202-70 - The Treasury Mentor-Protégé Program.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false The Treasury Mentor-Protégé Program. 1019.202-70 Section 1019.202-70 Federal Acquisition Regulations System DEPARTMENT OF THE TREASURY SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS Policies 1019.202-70 The Treasury...

  7. 48 CFR 3052.219-71 - DHS mentor-protégé program.

    Science.gov (United States)

    2010-10-01

    ..., which are small businesses, veteran-owned small businesses, service-disabled veteran-owned small businesses, HUBZone small businesses, small disadvantaged businesses, and women-owned small business concerns... providing developmental assistance to eligible small business protégé entities......

  8. Interactivity between protégés and scientists in an electronic mentoring program

    DEFF Research Database (Denmark)

    Bonnett, Cara; Wildemuth, Barbara M.; Sonnenwald, Diane H.

    2006-01-01

    in an electronic mentoring program. This report analyzes the interactivity between pairs of corporate research scientists (mentors) and university biology students (protégés) during two consecutive implementations of an electronic mentoring program. The frequency and structure of the interactions within each pair...

  9. protoDUNE-Single Phase and protDUNE-DualPhase

    CERN Multimedia

    Brice, Maximilien

    2016-01-01

    At the EHN1 two big 8m x 8m x8m detector prototypes (protoDUNE-Single Phase and protDUNE-DualPhase) are being constructed. The aim is to test technologies and detector performances for DUNE, a new generation of LBN neutr

  10. Students as Protégés: Factors That Lead to Success

    Science.gov (United States)

    Bear, Stephen; Jones, Gwen

    2017-01-01

    This study provides insight into the factors that influence satisfaction with an Internet-based practitioner-student mentoring relationship that is part of an undergraduate business school curriculum. Practitioner mentors are especially important because they can help student protégés learn the skills needed for their professional development,…

  11. Facilitating Protégé Career Development through Skills of Mentors

    Science.gov (United States)

    Balu, L.; James, Leena

    2017-01-01

    The success of protégé contribution in any organisation today depends more on the type of relationship that an organisation establishes with the support of mentors. Research shows that individuals who are mentored have an increased likelihood of career success as a result of the targeted developmental support they receive. Mentors serve as trusted…

  12. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II plus VII plus X activity in subjects infused with the drug. Influence of time and temperature

    DEFF Research Database (Denmark)

    Thorsen, S.; Teisner, A.; Jensen, S.A.;

    2009-01-01

    Objectives: The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences...

  13. Effect of N-acetylcysteine on the accuracy of the prothrombin time assay of plasma coagulation factor II+VII+X activity in subjects infused with the drug. Influence of time and temperature

    DEFF Research Database (Denmark)

    Thorsen, Sixtus; Teisner, Ane; Jensen, Søren Astrup;

    2009-01-01

    OBJECTIVES: The prothrombin time (PT) assay of factor II+VII+X activity is an important predictor of liver damage in paracetamol poisoned patients. It complicates interpretation of results that the antidote, acetylcysteine (NAC) depresses this activity. The aim was to investigate if NAC influences...

  14. Factor V Leiden mutation, prothrombin gene mutation, and deficiencies in coagulation inhibitors associated with Budd-Chiari syndrome and portal vein thrombosis: results of a case-control study

    NARCIS (Netherlands)

    H.L.A. Janssen (Harry); J.P. Vandenbroucke; F.R. Rosendaal (Frits); B. van Hoek (Bart); J.R. Meinardi; F.P. Vleggaar (Frank); S.H. van Uum; E.B. Haagsma (Els); F.J.M. van der Meer; J. van Hattum (Jan); R.A. Chamuleau; R.P.R. Adang (Rob)

    2000-01-01

    textabstractIn a collaborative multicenter case-control study, we investigated the effect of factor V Leiden mutation, prothrombin gene mutation, and inherited deficiencies of protein C, protein S, and antithrombin on the risk of Budd-Chiari syndrome (BCS) and portal vein thrombosi

  15. Infrastructure for the life sciences: design and implementation of the UniProt website

    Directory of Open Access Journals (Sweden)

    Suzek Baris E

    2009-05-01

    Full Text Available Abstract Background The UniProt consortium was formed in 2002 by groups from the Swiss Institute of Bioinformatics (SIB, the European Bioinformatics Institute (EBI and the Protein Information Resource (PIR at Georgetown University, and soon afterwards the website http://www.uniprot.org was set up as a central entry point to UniProt resources. Requests to this address were redirected to one of the three organisations' websites. While these sites shared a set of static pages with general information about UniProt, their pages for searching and viewing data were different. To provide users with a consistent view and to cut the cost of maintaining three separate sites, the consortium decided to develop a common website for UniProt. Following several years of intense development and a year of public beta testing, the http://www.uniprot.org domain was switched to the newly developed site described in this paper in July 2008. Description The UniProt consortium is the main provider of protein sequence and annotation data for much of the life sciences community. The http://www.uniprot.org website is the primary access point to this data and to documentation and basic tools for the data. These tools include full text and field-based text search, similarity search, multiple sequence alignment, batch retrieval and database identifier mapping. This paper discusses the design and implementation of the new website, which was released in July 2008, and shows how it improves data access for users with different levels of experience, as well as to machines for programmatic access. http://www.uniprot.org/ is open for both academic and commercial use. The site was built with open source tools and libraries. Feedback is very welcome and should be sent to help@uniprot.org. Conclusion The new UniProt website makes accessing and understanding UniProt easier than ever. The two main lessons learned are that getting the basics right for such a data provider website has

  16. The prothrombin time/international normalised ratio (PT/INR) line: derivation of local INR with commercial thromboplastins and coagulometers – two independent studies

    DEFF Research Database (Denmark)

    Poller, Leon; Ibrahim, S.; Keown, M.

    2011-01-01

    Background: The WHO scheme for prothrombin time (PT) standardization has been limited in application, because of its difficulties in implementation, particularly the need for mandatory manual PT testing and for local provision of thromboplastin international reference preparations (IRP). Methods...... thromboplastins and coagulometers. INRs were compared with manual certified values with thromboplastin IRP from expert centres and in the second study also with INRs from local ISI calibrations. Results: In the first study with the PT/INR Line, 8.7% deviation from certified INRs was reduced to 1.1% with human.......5% with bovine/combined reagents with local ISI calibrations and to 2.9% with the PT/INR Line. Mean INR dispersion was reduced with all thromboplastins and automated systems using the PT/INR Line. Conclusions: The procedure using the PT/INR Line provides reliable INR derivation without the need for WHO ISI...

  17. Antibodies to phosphatidylserine/prothrombin complex in suspected antiphospholipid syndrome in the absence of antibodies to cardiolipin or Beta-2-glycoprotein I.

    Science.gov (United States)

    Sanfelippo, M J; Joshi, A; Schwartz, Sl; Meister, J A; Goldberg, J W

    2013-11-01

    Antibodies to phosphatidylserine/prothrombin (aPS/PT) complex were measured in 728 serum specimens from patients suspected of having antiphospholipid syndrome (APS), but without diagnostic elevations in the levels of antibodies to cardiolipin or Beta-2 Glycoprotein 1 (β2-GP1). Of the 728 specimens, 41 had elevated levels of aPS/PT. Thrombotic events occurred in 11 of the 22 patients with accessible medical histories. Six of the patients with accessible medical records also had laboratory evidence of the lupus anticoagulant. The identification of aPS/PT in patients without evidence of antibodies to cardiolipin, β2-GP1, or the lupus anticoagulant can contribute to the identification of APS in patients that may go undetected with current testing methods.

  18. Expert curation in UniProtKB: a case study on dealing with conflicting and erroneous data.

    Science.gov (United States)

    Poux, Sylvain; Magrane, Michele; Arighi, Cecilia N; Bridge, Alan; O'Donovan, Claire; Laiho, Kati

    2014-01-01

    UniProtKB/Swiss-Prot provides expert curation with information extracted from literature and curator-evaluated computational analysis. As knowledgebases continue to play an increasingly important role in scientific research, a number of studies have evaluated their accuracy and revealed various errors. While some are curation errors, others are the result of incorrect information published in the scientific literature. By taking the example of sirtuin-5, a complex annotation case, we will describe the curation procedure of UniProtKB/Swiss-Prot and detail how we report conflicting information in the database. We will demonstrate the importance of collaboration between resources to ensure curation consistency and the value of contributions from the user community in helping maintain error-free resources. Database URL: www.uniprot.org.

  19. The Prothrombin G20210A Mutation is Associated with Young-Onset Stroke: The Genetics of Early Onset Stroke Study and Meta-Analysis

    Science.gov (United States)

    Jiang, Baijia; Ryan, Kathleen A.; Hamedani, Ali; Cheng, Yuching; Sparks, Mary J.; Koontz, Deborah; Bean, Christopher J.; Gallagher, Margaret; Hooper, W. Craig; McArdle, Patrick F.; O'Connell, Jeffrey R.; Stine, O. Colin; Wozniak, Marcella A.; Stern, Barney J.; Mitchell, Braxton D.; Kittner, Steven J.; Cole, John W.

    2014-01-01

    Background and Purpose Although the prothrombin G20210A mutation has been implicated as a risk factor for venous thrombosis, its role in arterial ischemic stroke is unclear, particularly among young-adults. To address this issue, we examined the association between prothrombin G20210A and ischemic stroke in a Caucasian case-control population and additionally performed a meta-analysis Methods From the population-based Genetics of Early Onset Stroke (GEOS) study we identified 397 individuals of European ancestry aged 15-49 years with first-ever ischemic stroke and 426 matched-controls. Logistic regression was used to calculate odds ratios in the entire population and for subgroups stratified by gender, age, oral contraceptive use, migraine and smoking status. A meta-analysis of 17 case-control studies (n=2305 cases stroke did not achieve statistical significance (OR=2.5,95%CI=0.9-6.5,p=0.07). However, among adults aged 15-42 (younger than median age), cases were significantly more likely than controls to have the mutation (OR=5.9,95%CI=1.2-28.1,p=0.03), whereas adults ages 42-49 were not (OR=1.4,95%CI=0.4-5.1,p=0.94). In our meta-analysis, the mutation was associated with significantly increased stroke risk in adults stroke in young-adults and may have an even stronger association among those with earlier onset strokes. Our finding of a stronger association in the younger-young adult population requires replication. PMID:24619398

  20. The relationship between factor V Leiden, prothrombin G20210A, and MTHFR mutations and the first major thrombotic episode in polycythemia vera and essential thrombocythemia.

    Science.gov (United States)

    Trifa, Adrian P; Cucuianu, Andrei; Popp, Radu A; Coadă, Camelia A; Costache, Roxana M; Militaru, Mariela S; Vesa, Ştefan C; Pop, Ioan V

    2014-02-01

    Arterial and venous thrombosis are the most frequent complications in patients with polycythemia vera and essential thrombocythemia. We sought to demonstrate a possible contribution of the factor V Leiden, prothrombin G20210A, and methylenetetrahydrofolate reductase (MTHFR) 677 C > T and 1298 A > C mutations to the thrombotic risk in patients with polycythemia vera and essential thrombocythemia along with other biological features of these patients. We included 86 patients with polycythemia vera, of which 34 (39.5 %) had major thrombosis and 95 patients with essential thrombocythemia, of which 22 (23.1 %) had major thrombosis. In the whole cohort of patients, only the factor V Leiden mutation was significantly associated with both arterial and venous thrombosis in univariate and multivariate analysis (odds ratio (OR) = 4.3; 95 % confidence interval (CI) = 1.5-12.5; p = 0.008 and OR = 4.3; 95 % CI = 1.2-15.9; p = 0.02, respectively). Other factors significantly associated with thrombosis in both univariate and multivariate analysis were male sex (OR = 2.8, 95 % CI = 1.4-5.4, p = 0.002 and OR = 3.5, 95 % CI = 1.6-7.6, p = 0.002, respectively) and the JAK2 V617F mutation (OR = 5.5, 95 % CI = 2.1-15, p = 0.0001 and OR = 6.9, 95 % CI = 2.2-21.2, p = 0.001, respectively). In conclusion, among the four mutations analyzed (factor V Leiden, prothrombin G20210A, and MTHFR 677 C > T and 1298 A > C), only factor V Leiden is a major contributor to thrombosis in polycythemia vera and essential thrombocythemia.

  1. The progress on preparation of human prothrombin complex concentrates%人凝血酶原复合物制备工艺研究现状

    Institute of Scientific and Technical Information of China (English)

    包正琦(综述); 何彦林(审校)

    2013-01-01

    Prothrombin complex concentrates are haemostatic blood products containing four vitamin K -dependent clotting factors (II, VII, IX and X).It is produced by ion-exchange chromatography on DEAE Sephadex A-50 resin from large pool plasma.Preparation technology,the choose of materials,quality control and optimization of critical parameters play a critical role in quality control of PCC .This paper mainly summarizes the preparation of PCC technology conditions , materi-als, critical parameters and the control strategy of coagulation factor activation .%人凝血酶原复合物( Prothrombin complex concentrates ,PCC)是一种含有凝血因子II、VII、IX、X四种维生素K依赖性凝血因子,能够促进血液凝固的血浆蛋白制剂。目前,多数生产厂家采用DEAE Sephadex A-50凝胶从混合血浆中制备PCC。 PCC制备工艺的选择、制备原料的挑选及质量控制、关键工艺参数的优化及控制等方面对PCC制品的质量至关重要。因此,主要对PCC的制备工艺、制备原料、关键工艺参数的优化及生产过程中凝血因子活化的控制策略进行了综述。

  2. Determinação da solubilidade protéica de farinhas de subproduto de aves com a pepsina em baixa concentração

    OpenAIRE

    Bellaver,Claudio; ZANOTTO,DIRCEU LUÍS; Guidoni,Antônio Lourenço

    2004-01-01

    Foi proposto recentemente que a solubilidade protéica in vitro com pepsina na concentração de 0,0002% é melhor que em concentrações maiores, para melhor classificar a qualidade protéica de farinhas de origem animal (FOA). Entretanto, nessa concentração, desconhece-se qual é o intervalo que melhor exprime a qualidade por meio da solubilidade protéica das FOA. Este trabalho foi conduzido com o objetivo de identificar os valores da solubilidade protéica de farinhas de subprodutos do abate de ave...

  3. Protéger les nourrissons contre la coqueluche

    Science.gov (United States)

    Gilley, Meghan; Goldman, Ran D.

    2014-01-01

    Résumé Question Compte tenu du taux à la hausse de la coqueluche chez les enfants, plusieurs familles m’ont demandé quels moyens prendre pour protéger leurs tout-petits de cette infection. Quelles devraient être mes recommandations à ces familles? Réponse La coqueluche est une maladie évitable qui est endémique dans le monde entier. Chez les adultes, la coqueluche cause une maladie bénigne semblable à un rhume, suivie d’une toux persistante. Chez les nourrissons, elle peut causer de l’apnée, des convulsions, une encéphalopathie, une bronchopneumonie et la mort. Les décès dus à la coqueluche se produisent dans 86 % des cas chez des nourrissons de moins de 4 mois. La stratégie du cocooning, c’est-à-dire la vaccination des adultes en étroit contact avec des nourrissons, est recommandée par de nombreuses agences mondiales et nationales, mais elle ne prévient probablement que 20 % des cas de coqueluche chez les nourrissons. La vaccination durant la grossesse est plus efficace, mais elle n’est pas encore approuvée au Canada. Il n’a pas été démontré que la vaccination à la naissance soit uniformément efficace et elle n’est donc pas recommandée à l’heure actuelle.

  4. Avaliação da qualidade protéica de dois suplementos alimentares em ratos Wistar

    Directory of Open Access Journals (Sweden)

    M. M. V. NAVES

    2008-10-01

    Full Text Available

    O objetivo da pesquisa foi avaliar a qualidade protéica de dois suplementos alimentares - um, formulado protéico em pó (produto em fase de teste e gelatina. Seis grupos de seis ratos machos Wistar recém-desmamados foram alimentados durante catorze dias com as seguintes dietas: controle (C10 - caseína, formulado protéico (FP10 e gelatina (G10 ao nível de 10 % de proteína; misturas de caseíma com formulado protéico (C7 FP3 ou gelatina (C7G3 (7:3 em base protéica, respectivamente; e dieta aprotéica. As dietas contendo o formulado protéico apresentaram boa qualidade protéica, segundo os índices NPR (Net Protein Ratio e NPU (Net Protein Utilization, sem diferenças significativas entre C7 FP3 e C10 (NPR= 4,00 e 4,11, e NPU= 65% e 67%, respectivamente. Os animais alimentados com gelatina (G10 e com dieta aprotéica perderam peso corpóreo e apresentaram pesos semelhantes dos fígados e inferiores aos dos demais grupos de ratos. O valor protéico da mistura caseína-gelatina (C7G3 foi menor que o da caseína (NPR relativo= 77 %, p<0,05. O formulado protéico constitui uma fonte de proteína de boa qualidade e seu uso como suplemento alimentar pode ser indicado em situações especiais. A gelatina, por sua vez, não pode ser considerada um complemento ou suplemento nutricional, pois não atende às necessidades protéicas do organismo e pode prejudicar a disponibilidade biológica de proteínas de boa qualidade.

  5. Ontology Development and Query Retrieval using Protégé Tool

    Directory of Open Access Journals (Sweden)

    Vishal Jain

    2013-08-01

    Full Text Available This paper highlights the explicit description about concept of ontology which is concerned with the development and methodology involved in building ontology. The concept of ontologies has contributed to the development of Semantic Web where Semantic Web is an extension of the current World Wide Web in which information is given in a well-defined meaning that translates the given unstructured data into knowledgeable representation data thus enabling computers and people to work in cooperation. Thus, we can say that Semantic Web is information in machine understandable form. It is also called as Global Information Mesh (GIM. Semantic Web technology can be used to deal with challenges including traditional search engines and retrieval techniques within given organizations or for e-commerce applications whose initial focus is on professional users. Ontology represents information in a manner so that this information can also be used by machines not only for displaying, but also for automating, integrating, and reusing the same information across various applications which may include Artificial Intelligence, Information Retrieval (IR and many more. Ontology is defined as a collection of set of concepts, their definitions and the relationships among them represented in a hierarchical manner that is termed as Taxonomy. There are various tools available for developing ontologies like Hozo, DOML, and AltovaSemantic Works etc. We have used protégé which is one of the most widely used ontology development editor that defines ontology concepts (classes, properties, taxonomies, various restrictions and class instances. It also supports several ontology representation languages, including OWL. There are various versions of protégé available like WebProtege 2.0 beta, Protégé 3.4.8, Protégé 4.1 etc. In this paper, we have illustrated ontology development using protégé 3.1 by giving an example of Computer Science Department of University System. It

  6. Plasma levels of plasminogen activator inhibitor type 1, factor VIII, prothrombin activation fragment 1+2, anticardiolipin, and antiprothrombin antibodies are risk factors for thrombosis in hemodialysis patients.

    Science.gov (United States)

    Molino, Daniela; De Santo, Natale G; Marotta, Rosa; Anastasio, Pietro; Mosavat, Mahrokh; De Lucia, Domenico

    2004-09-01

    Patients with end-stage renal disease are prone to hemorrhagic complications and simultaneously are at risk for a variety of thrombotic complications such as thrombosis of dialysis blood access, the subclavian vein, coronary arteries, cerebral vessel, and retinal veins, as well as priapism. The study was devised for the following purposes: (1) to identify the markers of thrombophilia in hemodialyzed patients, (2) to establish a role for antiphospholipid antibodies in thrombosis of the vascular access, (3) to characterize phospholipid antibodies in hemodialysis patients, and (4) to study the effects of dialysis on coagulation cascade. A group of 20 hemodialysis patients with no thrombotic complications (NTC) and 20 hemodialysis patients with thrombotic complications (TC) were studied along with 400 volunteer blood donors. Patients with systemic lupus erythematosus and those with nephrotic syndrome were excluded. All patients underwent a screening prothrombin time, activated partial thromboplastin time, fibrinogen (Fg), coagulation factors of the intrinsic and extrinsic pathways, antithrombin III (AT-III), protein C (PC), protein S (PS), resistance to activated protein C, prothrombin activation fragment 1+2 (F1+2), plasminogen, tissue type plasminogen activator (t-PA), plasminogen tissue activator inhibitor type-1 (PAI-1), anticardiolipin antibodies type M and G (ACA-IgM and ACA-IgG), lupus anticoagulant antibodies, and antiprothrombin antibodies type M and G (aPT-IgM and aPT-IgG). The study showed that PAI-1, F 1+2, factor VIII, ACA-IgM, and aPT-IgM levels were increased significantly over controls both in TC and NTC, however, they could distinguish patients with thrombotic complications from those without, being increased maximally in the former group. The novelty of the study is represented by the significant aPT increase that was observed in non-systemic lupus erythematosus hemodialysis patients, and particularly in those with thrombotic events. In addition

  7. Prevalence of factor V Leiden and prothrombin G20210A mutation in a large French population selected for nonthrombotic history: geographical and age distribution.

    Science.gov (United States)

    Mazoyer, Elisabeth; Ripoll, Laurent; Gueguen, René; Tiret, Laurence; Collet, Jean-Philippe; dit Sollier, Claire Bal; Roussi, Jacqueline; Drouet, Ludovic

    2009-10-01

    Among inherited risk factors for venous thrombosis, the most common are the FV-G1691A and FII-G20210A polymorphisms. The FV-G1691A polymorphism is preferentially observed in Europe, with differences between European countries. The FII-G20210A polymorphism is observed all over the world. The study was designed to compare the prevalence of the FV-G1691A and FII-G20210A polymorphisms in a large French population of unrelated individuals with no thrombotic disease history and to determine the age and geographical distributions. Over a period of 18 months, 6154 individuals were included throughout France and FV-G1691A and FII-G20210A polymorphisms were determined. The FV-G1691A prevalence was 3.84% (95% confidence interval 3.35-4.33) and the FII-G20210A prevalence was 3.07% (95% CI 2.63-3.51). A north-east/south-west gradient was observed in the FV-G1691A geographical distribution. No difference was observed in the geographical distribution of FII-G20210A polymorphism nor in the age distribution of the two polymorphisms. The prevalence of the two polymorphisms was similar whatever the blood group (O or non-O). Plasma D-dimers were significantly higher in healthy individuals with FV-G1691A but not in individuals with FII-G20210A. Thirty percent of variation in plasma prothrombin level was explained by environmental factors (serum cholesterol, age, oral contraception, hormonal replacement therapy, body mass index, sex) and genetic factors (FII-G20210A). As expected, individuals with FII-G20210A displayed higher plasma prothrombin level compared with individuals with wild type. However, this was not associated with a modification of the fibrin clot elastic modulus. This study shows a differential distribution of the two polymorphisms among the French territory. These polymorphisms confer a very mild hypercoagulable state as shown by the limited increased in basal D-dimers in mutated FV-G1691A populations and only a trend that does not reach statistical significance for FII

  8. The neXtProt peptide uniqueness checker: a tool for the proteomics community.

    Science.gov (United States)

    Schaeffer, Mathieu; Gateau, Alain; Teixeira, Daniel; Michel, Pierre-André; Zahn-Zabal, Monique; Lane, Lydie

    2017-05-17

    The neXtProt peptide uniqueness checker allows scientists to define which peptides can be used to validate the existence of human proteins, i.e. map uniquely versus multiply to human protein sequences taking into account isobaric substitutions, alternative splicing and single amino acid variants. The pepx program is available at https://github.com/calipho-sib/pepx and can be launched from the command line or through a cgi web interface. Indexing requires a sequence file in FASTA format. The peptide uniqueness checker tool is freely available on the web at https://www.nextprot.org/tools/peptide-uniqueness-checker and from the neXtProt API at https://api.nextprot.org /. lydie.lane@sib.swiss.

  9. Protéines végétales et risque allergique

    OpenAIRE

    Larré, Colette; Brossard, Chantal; Denery, Sandra; Lollier, Virginie; Lupi, Roberta

    2016-01-01

    L'augmentation de la part des protéines végétales dans l'alimentation humaine est une des réponses aux enjeux économiques, nutritionnels et écologiques des prochaines décennies. La connaissance actuelle des mécanismes et des molécules impliquées dans l’allergie alimentaire suggère toutefois un risque d’augmenter simultanément la prévalence de cette pathologie. Les familles protéiques fréquemment consommées et dans lesquelles se répartissent la majorité des allergènes alimentaires ont une cons...

  10. protVirt: protein dosage simulation by spectrometry assisting Biochemistry practical class

    Directory of Open Access Journals (Sweden)

    Gabriel Gerber Hornink

    2016-07-01

    Full Text Available The practical classes in the teaching of biochemistry could provide great contributions to the process of teaching and learning, and the understanding of these by students depend on, generally,  previous concepts about the experiment and procedures performed. It is presented in this paper the educational software protVirt, which could be used as a pedagogical innovation in the method of practical classes involving dosage of proteins, focusing on the development of skills for understanding the activities. The software was developed in Adobe Flash, with the possibility of online or offline use. Despite the possibilities of using protVirt in various modalities of education, highlights the use in online courses, in which students develop, commonly, the practical class without the teacher centers, accompanied by monitors and tutors.

  11. 48 CFR 719.273 - The U.S. Agency for International Development (USAID) Mentor-Protégé Program.

    Science.gov (United States)

    2010-10-01

    ... International Development (USAID) Mentor-Protégé Program. 719.273 Section 719.273 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT SOCIOECONOMIC PROGRAMS SMALL BUSINESS PROGRAMS The U.S. Agency for International Development (USAID) Mentor-Protégé Program 719.273 The U.S. Agency...

  12. Protégés' Personality Traits, Expectations, the Quality of the Mentoring Relationship and Adjustment: A Big Five Analysis

    Science.gov (United States)

    Goldner, Limor

    2016-01-01

    Background: Community-based mentoring interventions can benefit high-risk youth. However, meta-analyses suggest that these benefits may be conditioned by protégés' personality. Objectives: Associations between protégés' personality traits and mentoring expectations, the quality of the mentoring relationship, the perceived mentoring contribution,…

  13. Protégés' Personality Traits, Expectations, the Quality of the Mentoring Relationship and Adjustment: A Big Five Analysis

    Science.gov (United States)

    Goldner, Limor

    2016-01-01

    Background: Community-based mentoring interventions can benefit high-risk youth. However, meta-analyses suggest that these benefits may be conditioned by protégés' personality. Objectives: Associations between protégés' personality traits and mentoring expectations, the quality of the mentoring relationship, the perceived mentoring contribution,…

  14. ProtRepeatsDB: a database of amino acid repeats in genomes

    Directory of Open Access Journals (Sweden)

    Chauhan Virander S

    2006-07-01

    Full Text Available Abstract Background Genome wide and cross species comparisons of amino acid repeats is an intriguing problem in biology mainly due to the highly polymorphic nature and diverse functions of amino acid repeats. Innate protein repeats constitute vital functional and structural regions in proteins. Repeats are of great consequence in evolution of proteins, as evident from analysis of repeats in different organisms. In the post genomic era, availability of protein sequences encoded in different genomes provides a unique opportunity to perform large scale comparative studies of amino acid repeats. ProtRepeatsDB http://bioinfo.icgeb.res.in/repeats/ is a relational database of perfect and mismatch repeats, access to which is designed as a resource and collection of tools for detection and cross species comparisons of different types of amino acid repeats. Description ProtRepeatsDB (v1.2 consists of perfect as well as mismatch amino acid repeats in the protein sequences of 141 organisms, the genomes of which are now available. The web interface of ProtRepeatsDB consists of different tools to perform repeat s; based on protein IDs, organism name, repeat sequences, and keywords as in FASTA headers, size, frequency, gene ontology (GO annotation IDs and regular expressions (REGEXP describing repeats. These tools also allow formulation of a variety of simple, complex and logical queries to facilitate mining and large-scale cross-species comparisons of amino acid repeats. In addition to this, the database also contains sequence analysis tools to determine repeats in user input sequences. Conclusion ProtRepeatsDB is a multi-organism database of different types of amino acid repeats present in proteins. It integrates useful tools to perform genome wide queries for rapid screening and identification of amino acid repeats and facilitates comparative and evolutionary studies of the repeats. The database is useful for identification of species or organism specific

  15. Interactivity between protégés and scientists in an electronic mentoring program

    OpenAIRE

    Bonnett, C.; Wildemuth, B.; Sonnenwald, D. H.

    2006-01-01

    Interactivity is defined by Henri (1992) as a three-step process involving communication of information, a response to this information, and a reply to that first response. It is a key dimension of computer-mediated communication, particularly in the one-on-one communication involved in an electronic mentoring program. This report analyzes the interactivity between pairs of corporate research scientists (mentors) and university biology students (protégés) during two consecutive implementation...

  16. Management of Bleeding in Post-liver Disease, Surgery and Biopsy in Patients With High Uncorrected International Normalized Ratio With Prothrombin Complex Concentrate: An Iranian Experience.

    Science.gov (United States)

    Parand, Alireza; Honar, Naser; Aflaki, Khashayar; Imanieh, Mohammad Hadi; Haghighat, Mahmood; Cohan, Nader; Haghpanah, Sezaneh; Marietta, Marco; Serati, Zahra; Haghbin, Saeedeh; Karimi, Mehran

    2013-12-01

    To evaluate the efficacy of prothrombin complex concentrate (PCC) in the management of bleeding in patients with liver disease and patients undergoing surgery or biopsy who had a high uncorrected international normalized ratio (INR). In this study, we examined an Iranian sample and investigated the efficacy of PCC to manage bleeding in patients with liver disease and also patients with high uncorrected INR who were scheduled for surgery or biopsy. A total of 25 patients including 16 patients with post-liver disease bleeding (group 1) and 9 patients with high uncorrected INR who were scheduled for surgery or biopsy (group 2) were enrolled. All patients were treated with 25 IU/kg PCC, and efficacy was defined as any reduction in or cessation of bleeding episodes and correction of INR before surgery or biopsy. The patients were also evaluated for any adverse effects. INR decreased significantly in both groups of patients, with no bleeding episodes during or after the study in group 1 and during or after surgery/biopsy in group 2. All patients tolerated the therapy well without any significant adverse effects. The efficacy of PCC therapy was satisfactory in this study. PCC therapy in patients with liver disease and patients undergoing surgery or biopsy seems to be effective and safe, and may be a good treatment strategy for these patients, if fresh frozen plasma or vitamin K are not effective.

  17. Dietary factor VII activation does not increase plasma concentrations of prothrombin fragment 1+2 in patients with stable angina pectoris and coronary atherosclerosis

    DEFF Research Database (Denmark)

    Bladbjerg, E-M; Münster, A M; Marckmann, P

    2000-01-01

    for triglycerides, activated FVII (FVIIa), FVII protein concentration (FVII:Ag), prothrombin fragment 1+2 (F1+2), and soluble fibrin. Triglyceride levels increased from fasting levels on both diets, but they increased most markedly on the high-fat diet. FVIIa and FVIIa/FVII:Ag increased with the high-fat diet...... and decreased with the low-fat diet. For both diets, FVII:Ag and F1+2 decreased slightly. No postprandial changes were observed for soluble fibrin. Postprandial mean values of triglycerides, FVIIa, FVII:Ag, and FVIIa/FVII:Ag were significantly higher for the high-fat diet than for the low-fat diet. Our findings...... confirm that high-fat meals cause immediate activation of FVII. The clinical implication is debatable because FVII activation was not accompanied by an increase in plasma F1+2 concentrations in patients with severe atherosclerosis. However, a local thrombin generation on the plaque surface cannot...

  18. Maternal factor V Leiden and prothrombin mutations do not seem to contribute to the occurrence of two or more than two consecutive miscarriages in Caucasian patients.

    Science.gov (United States)

    Baumann, Kristin; Beuter-Winkler, Petra; Hackethal, Andreas; Strowitzki, Thomas; Toth, Bettina; Bohlmann, Michael K

    2013-12-01

    We analysed the prevalence of the most common hereditary thrombophilia (hTP) - factor V Leiden (FVL) mutation, prothrombin 20210 G>A substitution (PT) - and the 677 C>T replacement in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene in Caucasian patients with a history of two and more consecutive recurrent miscarriages (RMs) as compared to healthy controls with an identical ethnic background and at least one live birth. A multicenter analysis of three hTP was performed in 641 RM patients identically screened at specialized university centres. The study groups consisted of 240 patients with 2 (1) and 401 patients with >2 miscarriages (2) and were compared with 157 controls. There was no significant difference in the prevalence of the hTP between RM patients and controls nor within the two study groups. Subgroup analysis showed that the homozygous MTHFR polymorphism was significantly more prevalent in the study group 2 as compared to study group 1 (13.9 versus 7.9%, P = 0.02). In Caucasians, maternal FVL or PT mutations do not seem to contribute to the pathophysiology of RM, irrespective of the number of miscarriages. However, the role of the homozygous MTHFR polymorphism merits further investigation. © 2013 John Wiley & Sons Ltd.

  19. Factor V G1691A (Leiden and prothrombin G20210A gene mutation status, and thrombosis in patients with chronic myeloproliferative disorders

    Directory of Open Access Journals (Sweden)

    Nur Soyer

    2011-12-01

    Full Text Available Objective: The aim of this study was to examine Factor V G1691A (Leiden (FVL and prothrombin G20210A (PT gene mutation status, and their relationship with thrombosis in patients with chronic myeloproliferative disorders (CMPDs.Materials and Methods: The study included 160 patients with a CMPD that were regularly followed-up between 1993 and 2009. FVL and PT mutation status was established based on blood samples analyzed via PCR using specific primers.Results: The frequency of FVL and PT mutation was 12.5% and 4.4%, respectively. In total, 27 episodes of thrombosis occurred in 24 (15% of the patients, and there wasn’t an association between the observed thrombotic events, and FVL or PT mutations. Hepatic vein thrombosis was noted in 3 patients that had FVL mutation, of which 1 also had PT mutation.Conclusion: We did not observe a relationship between thrombosis, and FVL or PT mutations in CMPD patients; however, 3 of the patients that had hepatic vein thrombosis also had FVL mutation. Larger studies are needed to more clearly determine if all CMPD patients with hepatic vein thrombosis need be investigated for FVL and PT mutation.

  20. Thrombophilic risk of individuals with rare compound factor V Leiden and prothrombin G20210A polymorphisms: an international case series of 100 individuals.

    Science.gov (United States)

    Lim, Ming Y; Deal, Allison M; Kim, Steven; Musty, Michael D; Conard, Jacqueline; Simioni, Paolo; Dutrillaux, Fabienne; Eid, Suhair S; Middeldorp, Saskia; Halbmayer, Walter M; Boneu, Bernard; Moia, Marco; Moll, Stephan

    2016-10-01

    The risk of thrombosis in individuals with rare compound thrombophilias, homozygous factor V Leiden (FVL) plus heterozygous prothrombin G20210A (PTM), homozygous PTM plus heterozygous FVL, and homozygous FVL plus homozygous PTM, is unknown. We identified, worldwide, individuals with these compound thrombophilias, predominantly through mailing members of the International Society on Thrombosis and Haemostasis. Physicians were sent a clinical questionnaire. Confirmatory copies of the genetic results were obtained. One hundred individuals were enrolled; 58% were female. Seventy-one individuals had a venous thrombosis (includes superficial and deep vein thrombosis, and pulmonary embolism), 4 had an arterial thrombosis and 6 had both. Nineteen individuals had never had a thrombotic event. Thrombosis-free survival curves demonstrated that 50% of individuals had experienced a thrombotic event by 35 yrs of age, while 50% had a first venous thromboembolic event (VTE; includes all venous thrombosis except superficial thrombosis) by 41 yrs of age; 38.2% of first VTEs were unprovoked. 37% of patients had at least one VTE recurrence. Seventy percent of first pregnancies carried to term and not treated with anticoagulation were thrombosis-free. In conclusion, patients with these rare compound thrombophilias are not exceedingly thrombogenic, even though they have a substantial risk for VTE. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  1. Prothrombin fragment 1 + 2 (F1 + 2) in effluent is a useful marker for peritoneal permeability in peritoneal dialysis patients using neutral dialysate.

    Science.gov (United States)

    Sakurada, Tsutomu; Kojima, Shigeki; Oishi, Daisuke; Koitabashi, Kenichiro; Miyamoto, Masahito; Shirai, Sayuri; Shibagaki, Yugo; Yasuda, Takashi; Sato, Takeo; Kimura, Kenjiro

    2011-01-01

    To clarify the influence of neutral dialysate (ND) on peritoneum, we examined changes in peritoneal permeability and in various markers of the coagulation and fibrinolytic system in effluent and the correlations between peritoneal permeability and those markers in peritoneal dialysis (PD) patients using ND. We evaluated 14 patients (8 men, 6 women; mean age: 58.6 +/- 12.0 years) who started PD using ND. The peritoneal equilibration test (PET) was performed to assess dialysate-to-plasma ratio for creatinine (D/P Cr) as peritoneal permeability. Coagulation markers [thrombin-antithrombin complex, fibrin monomer (FM), prothrombin fragment 1+2 (F1 + 2)] and fibrinolytic markers (fibrin degradation products, D-dimer) in effluent were also measured. At 2 years, FM in effluent was significantly lower (p = 0.006). The other markers and the D/P Cr did not change significantly. At the initiation of PD and at 2 years, D/P Cr was significantly correlated with F1 + 2 (r = 0.70 and 0.76 respectively, p permeability in PD patients using ND.

  2. Rapid Regression of Advanced Hepatocellular Carcinoma Associated with Elevation of Des-Gamma-Carboxy Prothrombin after Short-Term Treatment with Sorafenib – A Report of Two Cases

    Directory of Open Access Journals (Sweden)

    Takahide Nakazawa

    2010-08-01

    Full Text Available Background: Sorafenib is the first molecular-targeted agent that is effective for advanced hepatocellular carcinoma (HCC, with prolongation of survival. However, a complete response is very rare, and rapid regression of HCC after short-term treatment with sorafenib has not been reported previously. Case Reports: We describe 2 patients with advanced multiple HCC who received sorafenib for short periods of 1 or 2 weeks, respectively. Longer treatment was precluded by the development of hepatic failure as an adverse event of sorafenib. Results: HCC rapidly regressed, and both patients had a partial response (PR, despite short-term treatment. Furthermore, an early elevation of des-gamma-carboxy prothrombin (DCP was temporarily seen in both patients, with no elevation of alpha-fetoprotein. Conclusions: Sorafenib can induce rapid regression of advanced HCC even after short-term treatment, and the initial response of HCC was identical in both patients. Since early elevation of DCP was observed in our patients with PR, DCP might be a predictive biomarker of anti-tumor response. Further studies are required to clarify the mechanisms underlying the effectiveness of sorafenib, including the alteration of DCP.

  3. The effects of vitamin K on the generation of des-gamma-carboxy prothrombin (PIVKA-II) in patients with hepatocellular carcinoma.

    Science.gov (United States)

    Sakon, M; Monden, M; Gotoh, M; Kobayashi, K; Kanai, T; Umeshita, K; Endoh, W; Mori, T

    1991-03-01

    The clinical significance of des-gamma-carboxy prothrombin (PIVKA-II) in hepatocellular carcinoma (HCC) was investigated in 112 patients with and without vitamin K administration. The positivity rate of PIVKA-II was significantly decreased in patients receiving vitamin K (28.5%), compared with those without vitamin K administration (54.5%, p less than 0.05). The plasma levels of vitamin K derivatives [phylloquinone (VK1), menaquinone-4 (MK4), and menaquinone-7 (MK7)] measured were not decreased in patients with HCC, but were significantly increased in MK4 and VK1 + MK4 + MK7. The amount of PIVKA-II in plasma did not correlate with the plasma levels of vitamin K derivatives. However, PIVKA-II was decreased by the administration of vitamin K, and all of the six patients with more than 5.0 ng/ml of VK1 + MK4 + MK7 were within normal limits, whereas half of 32 patients with less than that had abnormal levels of PIVKA-II. Thus, it was suggested that PIVKA-II was not elevated due to vitamin K deficiency, but might result from the impaired metabolism or availability of vitamin K in the tumor. Therefore, PIVKA-II should be measured without vitamin K administration.

  4. Análise das propriedades do PP injetado em molde-protótipo em Zamac-8 visando a obtenção de protótipos funcionais

    OpenAIRE

    Junkes, Vanderlei

    2015-01-01

    O uso de moldes-protótipo é uma opção para a obtenção de protótipos funcionais de peças de plástico injetadas. Estes protótipos devem possuir características mecânicas e físicas as mais próximas possíveis das peças finais obtidas em moldes de produção. A fim de diminuir o custo e o tempo de fabricação destes moldes, materiais alternativos, tais como o alumínio e ligas de zinco, podem ser empregados. Os diferentes níveis de condutividade térmica desses materiais resultam em diferentes taxas de...

  5. The association of factor V leiden and prothrombin gene mutation and placenta-mediated pregnancy complications: a systematic review and meta-analysis of prospective cohort studies.

    Directory of Open Access Journals (Sweden)

    Marc A Rodger

    2010-06-01

    Full Text Available BACKGROUND: Factor V Leiden (FVL and prothrombin gene mutation (PGM are common inherited thrombophilias. Retrospective studies variably suggest a link between maternal FVL/PGM and placenta-mediated pregnancy complications including pregnancy loss, small for gestational age, pre-eclampsia and placental abruption. Prospective cohort studies provide a superior methodologic design but require larger sample sizes to detect important effects. We undertook a systematic review and a meta-analysis of prospective cohort studies to estimate the association of maternal FVL or PGM carrier status and placenta-mediated pregnancy complications. METHODS AND FINDINGS: A comprehensive search strategy was run in Medline and Embase. Inclusion criteria were: (1 prospective cohort design; (2 clearly defined outcomes including one of the following: pregnancy loss, small for gestational age, pre-eclampsia or placental abruption; (3 maternal FVL or PGM carrier status; (4 sufficient data for calculation of odds ratios (ORs. We identified 322 titles, reviewed 30 articles for inclusion and exclusion criteria, and included ten studies in the meta-analysis. The odds of pregnancy loss in women with FVL (absolute risk 4.2% was 52% higher (OR = 1.52, 95% confidence interval [CI] 1.06-2.19 as compared with women without FVL (absolute risk 3.2%. There was no significant association between FVL and pre-eclampsia (OR = 1.23, 95% CI 0.89-1.70 or between FVL and SGA (OR = 1.0, 95% CI 0.80-1.25. PGM was not associated with pre-eclampsia (OR = 1.25, 95% CI 0.79-1.99 or SGA (OR 1.25, 95% CI 0.92-1.70. CONCLUSIONS: Women with FVL appear to be at a small absolute increased risk of late pregnancy loss. Women with FVL and PGM appear not to be at increased risk of pre-eclampsia or birth of SGA infants. Please see later in the article for the Editors' Summary.

  6. An evaluation of patient self-testing competency of prothrombin time for managing anticoagulation: pre-randomization results of VA Cooperative Study #481--The Home INR Study (THINRS).

    Science.gov (United States)

    Dolor, Rowena J; Ruybalid, R Lynne; Uyeda, Lauren; Edson, Robert G; Phibbs, Ciaran; Vertrees, Julia E; Shih, Mei-Chiung; Jacobson, Alan K; Matchar, David B

    2010-10-01

    Prior studies suggest patient self-testing (PST) of prothrombin time (PT) can improve the quality of anticoagulation (AC) and reduce complications (e.g., bleeding and thromboembolic events). "The Home INR Study" (THINRS) compared AC management with frequent PST using a home monitoring device to high-quality AC management (HQACM) with clinic-based monitoring on major health outcomes. A key clinical and policy question is whether and which patients can successfully use such devices. We report the results of Part 1 of THINRS in which patients and caregivers were evaluated for their ability to perform PST. Study-eligible patients (n = 3643) were trained to use the home monitoring device and evaluated after 2-4 weeks for PST competency. Information about demographics, medical history, warfarin use, medications, plus measures of numeracy, literacy, cognition, dexterity, and satisfaction with AC were collected. Approximately 80% (2931 of 3643) of patients trained on PST demonstrated competency; of these, 8% (238) required caregiver assistance. Testers who were not competent to perform PST had higher numbers of practice attempts, higher cuvette wastage, and were less able to perform a fingerstick or obtain blood for the cuvette in a timely fashion. Factors associated with failure to pass PST training included increased age, previous stroke history, poor cognition, and poor manual dexterity. A majority of patients were able to perform PST. Successful home monitoring of PT with a PST device required adequate levels of cognition and manual dexterity. Training a caregiver modestly increased the proportion of patients who can perform PST.

  7. Therapeutic concordance of two portable monitors and two routine automatic oral anticoagulant monitoring systems using as reference the manual prothrombin time technique.

    Science.gov (United States)

    Vacas, Marta; Lafuente, Pedro José; Unanue, Iciar; Santos, Mónica; Iriarte, Jose Antonio

    2003-01-01

    Two models of capillary blood prothrombin time (PT) monitoring systems were evaluated for analytical performance and then compared with two routine PT systems using the reference manual technique and a high-sensitivity thromboplastin. Two sets of 60 and 80 plasmas were analyzed from anticoagulated patients stabilized over 3 months in an INR range 2-3.5 for therapy. Capillary PT determination was performed in two portable monitors, CoaguChek S and CoaguChek PT (Roche Diagnostics), and plasma automatic methods were Neoplastine/STA (Diagnostics Stago) and PT-FibrinogenHsPlus/ACL7000 (Instrumental Laboratories). Thromboplastin Bilbao (TBi), an in-house high-sensitivity rabbit thromboplastin (ISI=1.08), recommended as the reference reagent by an External Spanish Oral Anticoagulant Quality Assessment, was used in the PT manual technique. The two monitors' coefficients of correlation with the reference system were 0.74 for CoaguChek S and 0.81 for CoaguChek PT. The automatic routine systems showed a correlation of 0.92 (Neoplastine/STA) and 0.91 (PT-FbHsPlus/ACL7000). Clinical agreement expressed as the percentage of simple correlation ranged between 75.0% (CoaguChek S) and 88.9% (Neoplastine/STA). The systems having the best kappa index with the manual technique were CoaguChek PT (71.9%) and the Neoplastine/STA system (73%). The routine PT management systems exhibited better correlation and percentage of concordance when using the TBi/manual technique than did the portable monitors, which moreover performed unequally in this regard.

  8. Propriétés fonctionnelles - Modifications chimiques des protéines alimentaires et étude des relations structure/fonction

    Directory of Open Access Journals (Sweden)

    Vidal Véronique

    2003-01-01

    Full Text Available Les modifications chimiques des protéines alimentaires sont réalisées dans le but d’ améliorer leurs propriétés fonctionnelles. Elles sont également pratiquées pour des études fondamentales des relations structure/fonction des protéines. Les principales modifications chimiques des protéines alimentaires sont présentées ainsi que leurs incidences sur les propriétés fonctionnelles telles les propriétés émulsifiantes, moussantes, visqueuses, d’hydratation et de gélification.

  9. Desempenho de cordeiros alimentados com diferentes níveis protéicos

    Directory of Open Access Journals (Sweden)

    Zundt Marilice

    2002-01-01

    Full Text Available O experimento foi conduzido com o objetivo de avaliar níveis crescentes de proteína bruta (PB na dieta de cordeiros em confinamento, por meio de seus efeitos sobre o desempenho animal. Trinta e dois cordeiros "tricross" (¹/2 Texel + fraction one-quarter Bergamácia + fraction one-quarter Corriedale, sendo 16 machos inteiros e 16 fêmeas, com idade média de 5 meses e peso vivo de 30 kg , foram alimentados durante 57 dias com dietas isoenergéticas (72% NDT, variando os níveis protéicos (12, 16, 20 e 24 % PB. Os animais foram identificados, pesados e distribuídos nos quatro tratamentos. Os cordeiros permaneceram em baias individuais, cobertas, com piso ripado e suspenso. Os parâmetros estudados foram ganho de peso, consumo de matéria seca, conversão alimentar e o custo de produção do quilograma de carcaça. A utilização de dietas, com diferentes níveis protéicos na terminação de cordeiros, mostrou efeito linear positivo para ganho de peso e negativo para conversão alimentar. Verificou-se que a utilização de dietas com níveis protéicos crescentes não influenciou o consumo de matéria seca dos cordeiros. Observou-se viabilidade econômica na terminação de cordeiros, utilizando-se dietas entre 12 e 24% de PB, entretanto, o maior retorno foi obtido com a ração contendo 12% PB. Não houve efeito de sexo para os parâmetros analisados.

  10. Propriedades funcionais de concentrado protéico extrusado de sementes de girassol

    Directory of Open Access Journals (Sweden)

    Elenice H. Murate

    1999-01-01

    Full Text Available Propriedades funcionais de concentrado protéico de sementes de girassol extrusado a 135, 143 e 151ºC e com 20 e 24% de umidade (base seca foram avaliadas. Os resultados demonstraram que a extrusão, dependendo das condições, provocou um aumento nos valores de solubilidade da proteína em água, capacidade de absorção de água e gordura, capacidade e atividade emulsificante, estabilidade da emulsão, capacidade de formação de espuma, porém não alterou a propriedade de estabilidade de espuma do concentrado protéico. A solubilidade das proteínas do concentrado protéico extrusado e não extrusado em função do pH foi menor no intervalo de 4,0 a 6,0 e houve um aumento significativo a partir do pH 7,0 para a amostra não extrusada.Functional properties of sunflower protein concentrate extruded at 135, 143 and 151°C with 20 and 24% moisture (dry basis were evaluated. Extrusion resulted in increasing protein solubility in water, fat and water absorption, emulsifying activity and capacity, emulsion stability and foaming capacity but, foam stability did not change. Protein solubility of extruded and non extruded protein concentrate, as a function of pH, were lower in the interval of 4.0 to 6.0, with a solubility increase above pH 7,0 for the non extruded concentrate.

  11. Anastomose cavo-pulmonar total sem uso de material protético

    Directory of Open Access Journals (Sweden)

    PINTO Jr. Valdester C.

    1998-01-01

    Full Text Available Objetivo: Demonstrar a viabilidade na feitura de túnel cava inferior-cava superior com retalho da parede atrial direita, evitando o emprego de material protético. Casuística e Métodos: Foram operados 2 pacientes nos quais se empregou a técnica de anastomose cavo-pulmonar total, sem uso de material protético. O primeiro caso, A.L.M., masc, 4 anos, 15 kg, era portador de atresia tricúspide (EP, com comunicação interventricular (CIV restritiva. O segundo caso, M.E.N.O., fem, 15 anos, 47 kg, tinha doença de Ebstein. O controle pós-operatório dos pacientes foi feito com ecocardiograma e cateterismo cardíaco. As operações foram realizadas com o emprego de circulação extracorpórea (CEC, e cardioplegia sangüínea como método de proteção miocárdica. A canulação das cavas foi o mais distal possível. A tunelização foi realizada com retalho de tecido atrial direito, suturado ao septo interatrial, deixando-se o seio coronariano e a comunicação interatrial (CIA para a esquerda. Resultados: Ambos os pacientes evoluíram, sem complicações, na UTI. O primeiro apresentou derrame pleural discreto à direita, e o segundo mantém-se em estimulação artificial (VVI,R. Conclusões: A tunelização intra-atrial para anastomose cavo-pulmonar total pode ser realizada sem o uso de material protético, evitando-se os riscos advindos do seu emprego (calcificação, retração, embolização.

  12. Building Course Content Ontology Based on Protégé%基于protégé的课程内容本体的构建

    Institute of Scientific and Technical Information of China (English)

    蔡群英; 黄镇建

    2012-01-01

    本体作为一种有效表现概念层次结构和相互关系的模型,能够有效地表示课程内容框架,以visual basic 课程为例,使用protégé工具进行建模,初步探讨了课程内容本体的构建原则和步骤.%Ontology, an efficient model of expressing concept hierarchy and relationship, can be used to express course content frame. This paper uses visual basic course as research object and using protege as a ontology construnction tool. The construction of the principle and steps has been discussed preliminary.

  13. ChemProt-2.0: visual navigation in a disease chemical biology database

    DEFF Research Database (Denmark)

    Kjærulff, Sonny Kim; Wich, Louis; Kringelum, Jens Vindahl

    2013-01-01

    measurements for 15 290 proteins. Each protein is linked to quality-scored human protein-protein interactions data based on more than half a million interactions, for studying diseases and biological outcomes (diseases, pathways and GO terms) through protein complexes. In ChemProt-2.0, therapeutic effects...... of proteins, which can help in the prediction of off-target effects. Finally, the database was integrated into a visual interface that enables navigation of the pharmacological space for small molecules. Filtering options were included in order to facilitate and to guide dynamic search of specific queries....

  14. Últimas tendencias en cirugía protésica de rodilla

    OpenAIRE

    Silvestre Muñoz, Antonio; Peña, F.; López Lozano, Raúl; Carratalá Baixauli, V.

    2015-01-01

    En los últimos años hemos asistido a una importante evolución en los diseños protésicos de la artroplastia total de rodilla. Todos ellos tienen como finalidad mejorar los satisfactorios resultados de este procedimiento. La búsqueda del par de fricción ideal en este implante ha dado como resultado las mejoras en el proceso de fabricación del inserto e incluso la incorporación de nuevos materiales empleados con éxito en otras articulaciones, pero cuyos beneficios en la rodilla todav...

  15. Obtención de hidrolizados protéicos a partir de cebada agotada

    OpenAIRE

    Belagardi, Mariela Irene

    2011-01-01

    Una forma importante de reutilizar lso residuos agroindustriales y producir productos de valor agregado consiste en la producción de hidrolizados protéicos. En este trabajo se utilizó como sustrato grano agotado de cerveza, BGS por sus siglas. Los objetivos principales fueron en una primera etapa, realizar una caracterización físico-química y microbiológica de de tres variedades de BSG. En una segunda parte, se llevó a cabo la preparación preliminar y estandarización del BSG a través de una s...

  16. The significance of the ProtDeform score for structure prediction and alignment.

    Directory of Open Access Journals (Sweden)

    Jairo Rocha

    Full Text Available BACKGROUND: When a researcher uses a program to align two proteins and gets a score, one of her main concerns is how often the program gives a similar score to pairs that are or are not in the same fold. This issue was analysed in detail recently for the program TM-align with its associated TM-score. It was shown that because the TM-score is length independent, it allows a P-value and a hit probability to be defined depending only on the score. Also, it was found that the TM-scores of gapless alignments closely follow an Extreme Value Distribution (EVD. The program ProtDeform for structural protein alignment was developed recently and is characterised by the ability to propose different transformations of different protein regions. Our goal is to analyse its associated score to allow a researcher to have objective reasons to prefer one aligner over another, and carry out a better interpretation of the output. RESULTS: The study on the ProtDeform score reveals that it is length independent in a wider score range than TM-scores and that PD-scores of gapless (random alignments also approximately follow an EVD. On the CASP8 predictions, PD-scores and TM-scores, with respect to native structures, are highly correlated (0.95, and show that around a fifth of the predictions have a quality as low as 99.5% of the random scores. Using the Gold Standard benchmark, ProtDeform has lower probabilities of error than TM-align both at a similar speed. The analysis is extended to homology discrimination showing that, again, ProtDeform offers higher hit probabilities than TM-align. Finally, we suggest using three different P-values according to the three different contexts: Gapless alignments, optimised alignments for fold discrimination and that for superfamily discrimination. In conclusion, PD-scores are at the very least as valuable for prediction scoring as TM-scores, and on the protein classification problem, even more reliable.

  17. 基于 protégé的中医证候本体构建方法研究%Researchon Construction Methods of Pattern Ontology Based on Protégé

    Institute of Scientific and Technical Information of China (English)

    李明; 朱邦贤; 周强

    2015-01-01

    Protégéis a free,open source ontology editor and framework for building intelligent systems. according to the protégé’s features,attributes and knowledge representation to build Pattern Ontology,we took protégéas a tool of knowledge representation,and analyzed the definitions of the concept systems of TCM pattern that based on Resource Description Framework.Based on upper-level ontology of TCM,this paper built pattern ontology from the aspects of construction principles of pattern ontology,upper-level on-tology,formal definition,formal description,principles of category,property and relations.While,this pa-per studied the integration of TCM ontology and the other ontologies,studied the formal expression of Tra-ditional Chinese Medicine and its impact on standardization of TCM terminology.%Protégé是本体构建工具中应用最为广泛的一种开源软件。本研究以 protégé作为知识表示的工具,根据 protégé的功能特点、属性及其知识表示方法,以 RDF 三元组作为中医证候本体的描述框架,在已有的中医顶层本体的基础上,从中医本体的构建原则、顶层设计、形式化定义、形式化描述、类别体系、基于 protégé知识表示方法的中医证候本体中的关系和属性等几个方面探讨构建概念明确、层次清楚、结构清晰的中医证候本体,研究中医本体的构建方法及与其它本体的整合方式、探讨中医术语的形式化表达及其对中医术语标准化的影响。

  18. 48 CFR 1052.219-73 - Department of the Treasury Mentor-Protégé Program.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Department of the Treasury... DEPARTMENT OF THE TREASURY CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 1052.219-73 Department of the Treasury Mentor-Protégé Program. As described in DTAR...

  19. ProtSA: a web application for calculating sequence specific protein solvent accessibilities in the unfolded ensemble

    Directory of Open Access Journals (Sweden)

    Blackledge Martin

    2009-04-01

    Full Text Available Abstract Background The stability of proteins is governed by the heat capacity, enthalpy and entropy changes of folding, which are strongly correlated to the change in solvent accessible surface area experienced by the polypeptide. While the surface exposed in the folded state can be easily determined, accessibilities for the unfolded state at the atomic level cannot be obtained experimentally and are typically estimated using simplistic models of the unfolded ensemble. A web application providing realistic accessibilities of the unfolded ensemble of a given protein at the atomic level will prove useful. Results ProtSA, a web application that calculates sequence-specific solvent accessibilities of the unfolded state ensembles of proteins has been developed and made freely available to the scientific community. The input is the amino acid sequence of the protein of interest. ProtSA follows a previously published calculation protocol which uses the Flexible-Meccano algorithm to generate unfolded conformations representative of the unfolded ensemble of the protein, and uses the exact analytical software ALPHASURF to calculate atom solvent accessibilities, which are averaged on the ensemble. Conclusion ProtSA is a novel tool for the researcher investigating protein folding energetics. The sequence specific atom accessibilities provided by ProtSA will allow obtaining better estimates of the contribution of the hydrophobic effect to the free energy of folding, will help to refine existing parameterizations of protein folding energetics, and will be useful to understand the influence of point mutations on protein stability.

  20. Race and Gender Mentor-Protégé Pairings of Resident Assistants: An Exploration on Leadership Capacity

    Science.gov (United States)

    Early, Sherry L.

    2017-01-01

    The leadership capacity of resident assistants can be impacted by many experiences, including involvement in mentoring relationships. The purpose of this study was to examine if and how resident assistants' leadership capacities are influenced by participating in mentoring relationships. Additionally, mentor-protégé race and gender pairings were…

  1. Risk of placenta-mediated pregnancy complications or pregnancy-related VTE in VTE-asymptomatic families of probands with VTE and heterozygosity for factor V Leiden or G20210 prothrombin mutation.

    Science.gov (United States)

    Cordoba, Iris; Pegenaute, Carlota; González-López, Tomás José; Chillon, Carmen; Sarasquete, Maria Eugenia; Martin-Herrero, Francisco; Guerrero, Carmen; Cabrero, Mónica; Garcia Sanchez, Maria Helena; Pabon, Pedro; Lozano, Francisco Santiago; Gonzalez, Marcos; Alberca, Ignacio; González-Porras, José Ramón

    2012-09-01

    Few studies have evaluated the risk of pregnancy-related adverse events in asymptomatic relatives of probands for VTE and factor V Leiden or the G20210A variant. The antepartum management of this population ranges from antepartum anticoagulation therapy to clinical surveillance. To evaluate the risk of placenta-mediated pregnancy complications and pregnancy-related VTE in VTE-asymptomatic families of probands with VTE and who are heterozygous carriers of either factor V Leiden or PT-G20210A mutation. One hundred and fifty-eight relatives, who had 415 pregnancies, were retrospectively evaluated. Odds ratios and 95% confidence intervals were calculated to compare pregnancy outcomes between women with and without thrombophilia. In the factor V Leiden group, 22 placenta-mediated pregnancy events of 152 pregnancies (14.4%) were reported, compared with 25 adverse events of 172 pregnancies in the G20210A prothrombin group (14.5%) and 13 adverse events of 91 pregnancies in the non-carrier group (14.2%). Carriers of factor V Leiden or G20210A prothrombin were not associated with a higher risk of pregnancy-adverse outcomes compared with non-carriers: OR 1.02 (95% CI, 0.40-2.25) and 1.25 (95% CI, 0.48-3.24), respectively. Four episodes of pregnancy-associated VTE of 415 pregnancies (0.96%) were recorded. Two episodes of VTE in the G20210A group, one in the factor V Leiden group, and one episode in the non-carrier group were noted. In VTE-asymptomatic relatives of probands with VTE, the presence of factor V Leiden or the G20210A prothrombin mutation in heterozygosis should not lead to a decision to instigate antepartum prophylaxis. © 2012 John Wiley & Sons A/S.

  2. Local verification and assignment of mean normal prothrombin time and International Sensitivity Index values across various instruments: recent experience and outcome from North America.

    Science.gov (United States)

    Tange, Julie I; Grill, Diane; Koch, Christopher D; Ybabez, Roxanne J; Krekelberg, Benjamin J; Fylling, Kara A; Wiese, Cynthia R; Baumann, Nikola A; Block, Darci R; Karon, Brad S; Chen, Dong; Pruthi, Rajiv K

    2014-02-01

    Warfarin dosing relies on accurate measurements of international normalized ratio (INR), which is calculated from the prothrombin time (PT), International Sensitivity Index international sensitivity index (ISI) of the thromboplastin, and the geometric mean of normal PT (MNPT). However, ISI assignments of certain reagent/instrument combinations are frequently unavailable, especially when the reagent and instrument are not from the same manufacturer. The effort to be in compliance with widely endorsed Clinical and Laboratory Standards Institute (CLSI) guidelines by locally verifying or assigning an ISI to an unsupported reagent/instrument combination is further hindered by the lack of US Food and Drug Administration (FDA)-approved certified plasmas designated for a particular reagent/instrument combination. The objectives of the study include development of a process to verify/assign ISI and MNPT of a single thromboplastin reagent from one manufacturer across multiple instruments including several from another manufacturer and across several campuses of a single institution, the Mayo Clinic. In this study, RecombiPlasTin 2G (R2G), was evaluated on the ACL TOP 700 (IL), STA-R Evolution, STA Compact, and STA Satellite. Random normal donor samples (n = 25) were used to verify/assign MNPT. A subset of the normal donors (n = 8) and 13 warfarin pools (INR range: 1.3-3.9), created from stable warfarin patient plasma, were used for ISI verification/assignment. The manufacturer's assigned ISI was first verified on the ACL TOP 700 (reference method), then assigned on three unsupported instruments using orthogonal regression analysis. The MNPT and manufacturer assigned ISI (11.0, 0.95) were verified on the ACL TOP 700 and subsequently assigned on the STA-R Evolution (11.6, 1.04); STA Compact (11.5, 1.02); and STA Satellite (10.9, 0.99). Linear correlations of the INR results from all the four instruments demonstrated an r2 > 0.99. This process provides a reproducible approach

  3. xPROT : NESNE-YÖNELİMLİ PROTOTİPLEME MODELİ

    Directory of Open Access Journals (Sweden)

    A. Nuri BAŞOĞLU

    2000-01-01

    Full Text Available İlişkisel veri tabanları bilgiişlem uygulamalarında standart olmaya başlamasına rağmen, karmaşık veri modellemesinin gereklerini yerine getirmek için nesne yönelimli sistemler geliştirilmektedir. Bu makalede, hızlı program geliştirmek için kullanılabilecek, nesne-tabanlı bir sistem, xPROT tanıtılmaktadır. Sistem bazı önceden tanımlanmış metod ve Menu, Report, View, Table gibi sınıfları içermektedir. xPROT'da veri şeması olduğu kadar, veri işleme de aynı komut bazlı dil ile ifade edilmektedir. Sistemin ifade yeterliliği, kolaylığı ve esnekliği, veri modelleme gücü açısından incelenmektedir.

  4. Prediction of Metabolic Pathway Involvement in Prokaryotic UniProtKB Data by Association Rule Mining

    KAUST Repository

    Boudellioua, Imane

    2016-07-08

    The widening gap between known proteins and their functions has encouraged the development of methods to automatically infer annotations. Automatic functional annotation of proteins is expected to meet the conflicting requirements of maximizing annotation coverage, while minimizing erroneous functional assignments. This trade-off imposes a great challenge in designing intelligent systems to tackle the problem of automatic protein annotation. In this work, we present a system that utilizes rule mining techniques to predict metabolic pathways in prokaryotes. The resulting knowledge represents predictive models that assign pathway involvement to UniProtKB entries. We carried out an evaluation study of our system performance using cross-validation technique. We found that it achieved very promising results in pathway identification with an F1-measure of 0.982 and an AUC of 0.987. Our prediction models were then successfully applied to 6.2 million UniProtKB/TrEMBL reference proteome entries of prokaryotes. As a result, 663,724 entries were covered, where 436,510 of them lacked any previous pathway annotations.

  5. SynProt: A Comprehensive Database for Proteins of the Detergent-Resistant Synaptic Junctions Fraction

    Directory of Open Access Journals (Sweden)

    Rainer ePielot

    2012-06-01

    Full Text Available Chemical synapses are highly specialized cell-cell contacts for communication between neurons in the CNS characterized by complex and dynamic protein networks at both synaptic membranes. The cytomatrix at the active zone (CAZ organizes the apparatus for the regulated release of transmitters from the presynapse. At the postsynaptic side, the postsynaptic density constitutes the machinery for detection, integration and transduction of the transmitter signal. Both pre- and postsynaptic protein networks represent the molecular substrates for synaptic plasticity. Their function can be altered both by regulating their composition and by post-translational modification of their components. For a comprehensive understanding of synaptic networks the entire ensemble of synaptic proteins has to be considered. To support this, we established a comprehensive database for synaptic junction proteins (SynProt database primarily based on proteomics data obtained from biochemical preparations of detergent-resistant synaptic junctions. The database currently contains 2,788 non-redundant entries of rat, mouse and some human proteins, which mainly have been manually extracted from twelve proteomic studies and annotated for synaptic subcellular localization. Each dataset is completed with manually added information including protein classifiers as well as automatically retrieved and updated information from public databases (UniProt and PubMed. We intend that the database will be used to support modeling of synaptic protein networks and rational experimental design.

  6. SynProt: A Database for Proteins of Detergent-Resistant Synaptic Protein Preparations

    Science.gov (United States)

    Pielot, Rainer; Smalla, Karl-Heinz; Müller, Anke; Landgraf, Peter; Lehmann, Anne-Christin; Eisenschmidt, Elke; Haus, Utz-Uwe; Weismantel, Robert; Gundelfinger, Eckart D.; Dieterich, Daniela C.

    2012-01-01

    Chemical synapses are highly specialized cell–cell contacts for communication between neurons in the CNS characterized by complex and dynamic protein networks at both synaptic membranes. The cytomatrix at the active zone (CAZ) organizes the apparatus for the regulated release of transmitters from the presynapse. At the postsynaptic side, the postsynaptic density constitutes the machinery for detection, integration, and transduction of the transmitter signal. Both pre- and postsynaptic protein networks represent the molecular substrates for synaptic plasticity. Their function can be altered both by regulating their composition and by post-translational modification of their components. For a comprehensive understanding of synaptic networks the entire ensemble of synaptic proteins has to be considered. To support this, we established a comprehensive database for synaptic junction proteins (SynProt database) primarily based on proteomics data obtained from biochemical preparations of detergent-resistant synaptic junctions. The database currently contains 2,788 non-redundant entries of rat, mouse, and some human proteins, which mainly have been manually extracted from 12 proteomic studies and annotated for synaptic subcellular localization. Each dataset is completed with manually added information including protein classifiers as well as automatically retrieved and updated information from public databases (UniProt and PubMed). We intend that the database will be used to support modeling of synaptic protein networks and rational experimental design. PMID:22737123

  7. Teachable Agents and the Protégé Effect: Increasing the Effort Towards Learning

    Science.gov (United States)

    Chase, Catherine C.; Chin, Doris B.; Oppezzo, Marily A.; Schwartz, Daniel L.

    2009-08-01

    Betty's Brain is a computer-based learning environment that capitalizes on the social aspects of learning. In Betty's Brain, students instruct a character called a Teachable Agent (TA) which can reason based on how it is taught. Two studies demonstrate the protégé effect: students make greater effort to learn for their TAs than they do for themselves. The first study involved 8th-grade students learning biology. Although all students worked with the same Betty's Brain software, students in the TA condition believed they were teaching their TAs, while in another condition, they believed they were learning for themselves. TA students spent more time on learning activities (e.g., reading) and also learned more. These beneficial effects were most pronounced for lower achieving children. The second study used a verbal protocol with 5th-grade students to determine the possible causes of the protégé effect. As before, students learned either for their TAs or for themselves. Like study 1, students in the TA condition spent more time on learning activities. These children treated their TAs socially by attributing mental states and responsibility to them. They were also more likely to acknowledge errors by displaying negative affect and making attributions for the causes of failures. Perhaps having a TA invokes a sense of responsibility that motivates learning, provides an environment in which knowledge can be improved through revision, and protects students' egos from the psychological ramifications of failure.

  8. Tendance de marché en France sur la présence des protéines végétales dans les produits alimentaires

    Directory of Open Access Journals (Sweden)

    Estève-Saillard Morgane

    2016-07-01

    Full Text Available Le contexte actuel est favorable à l’utilisation croissante des protéines végétales dans les produits alimentaires : préoccupations sanitaires et environnementales, développement du flexitarisme et dans une moindre mesure du végétarisme, inscriptions des protéines végétales dans les secteurs de recherche stratégiques pour la France...Le bilan de référencement du GEPV, Groupe d’Étude et de Promotion des Protéines Végétales, fait le point tous les deux ans sur la présence des protéines végétales dans les produits alimentaires commercialisés en France. Les résultats du dernier bilan montrent la nette progression de l’utilisation de protéines végétales dans tous les rayons alimentaires, en progression constante depuis plusieurs années. Le blé reste la source largement majoritaire, suivi par le soja. Des spécificités se dégagent dans l’utilisation des ingrédients protéiques végétaux; par exemple les protéines de pois et de soja sont principalement utilisées pour les produits carnés alors que le gluten et la féverole sont surtout utilisés pour les produits de panification. De nouvelles utilisations des sources protéiques voient également le jour et feront l’objet d’un suivi du GEPV, comme les microalgues ou les mycoprotéines.

  9. KLHL41 stabilizes skeletal muscle sarcomeres by nonproteolytic ubiquitination

    Science.gov (United States)

    Ramirez-Martinez, Andres; Cenik, Bercin Kutluk; Bezprozvannaya, Svetlana; Chen, Beibei; Bassel-Duby, Rhonda

    2017-01-01

    Maintenance of muscle function requires assembly of contractile proteins into highly organized sarcomeres. Mutations in Kelch-like protein 41 (KLHL41) cause nemaline myopathy, a fatal muscle disorder associated with sarcomere disarray. We generated KLHL41 mutant mice, which display lethal disruption of sarcomeres and aberrant expression of muscle structural and contractile proteins, mimicking the hallmarks of the human disease. We show that KLHL41 is poly-ubiquitinated and acts, at least in part, by preventing aggregation and degradation of Nebulin, an essential component of the sarcomere. Furthermore, inhibition of KLHL41 poly-ubiquitination prevents its stabilization of nebulin, suggesting a unique role for ubiquitination in protein stabilization. These findings provide new insights into the molecular etiology of nemaline myopathy and reveal a mechanism whereby KLHL41 stabilizes sarcomeres and maintains muscle function by acting as a molecular chaperone. Similar mechanisms for protein stabilization likely contribute to the actions of other Kelch proteins. PMID:28826497

  10. Fragmento 1+2 da protrombina em indivíduos submetidos à angiografia coronariana Prothrombin fragment 1+2 in subjects undergoing coronary angiography

    Directory of Open Access Journals (Sweden)

    Luciana M. Lima

    2005-09-01

    transforms the prothrombin into thrombin and breaks up prothrombin fragment 1+2 (F1+2. F1+2 plasma levels reflect the thrombin generation and can be used as in vivo markers of hypercoagulability since the thrombin is an unstable and easily degraded substance that cannot be directly measured in the plasma. The present study aims at determining the F1+2 plasma levels of a group of subjects undergoing coronary angiography, attempting to establish a possible correlation between this parameter and the severity of the coronary artery disease. F1+2 plasma levels were determined in blood samples of 17 subjects with absence of atheromatosis in coronary arteries (controls, 12 subjects presenting mild/moderate atheromatosis and 28 subjects presenting severe atheromatosis, using the Enzignost F1+2 (Behring® Diagnostics GmbH, Marburg, Germany diagnostic Kit. Significant differences between the averages for the three groups in respect to the evaluated parameters were not found. Therefore, F1+2 plasma level averages for the three groups did not point to a state of hypercoagulability in the studied population. However, 73.7% of the individuals were taking acetylsalicylic acid, which may have influenced the F1+2 plasma levels, considering that this medicine promotes the inhibition of the enzyme cyclo-oxygenase, diminishing the release of thromboxane A2 and the platelet aggregation. Therefore, it is presumed that platelet activation reduction could be contributing to a lower formation of thrombin and, consequently, diminishing the hypercoagulability potential.

  11. Isoflavonas em isolados e concentrados protéicos de soja Isoflavones in soy protein isolate and soy protein concentrate

    Directory of Open Access Journals (Sweden)

    Maria Cristina Y. Lui

    2003-12-01

    Full Text Available Isolados e concentrados protéicos de soja são ingredientes largamente utilizados na indústria de panificação, confeitaria, bebidas e embutidos. As isoflavonas presentes na soja podem sofrer alterações em quantidade e perfil de distribuição dependendo das condições de processamento. O objetivo deste estudo foi verificar o balanço de massa de isoflavonas e proteína em processamento de isolados e de concentrados protéicos de soja (tratamento com ácido e com álcool. A maior parte das isoflavonas presentes na matéria-prima (farinha desengordurada de soja é perdida nos sobrenadantes de processo (90% para extração com etanol 60%, 52% para processamento de isolado protéico e 47% para extração com ácido. O teor de isoflavonas nos produtos obtidos foi de 686µg/g base seca (b.s. para isolado protéico, 871µ g/g b.s. para concentrado protéico obtido por tratamento ácido e apenas 153µg/g b.s. para concentrado protéico obtido por tratamento com álcool. Não foi observada alteração no perfil de distribuição das isoflavonas nesse último processo, enquanto que nos dois primeiros notou-se diminuição da quantidade das formas malonil glicosídeos e aumento da quantidade das formas beta-glicosiladas e gliconas.Soy protein isolate (SPI and soy protein concentrate (SPC are largely used in bakery, confectionary, meat and beverage products. Isoflavones present in soybeans products can undergo changes in quantity and profile depending on the processing conditions. The objective of this work was to conduct mass balance studies of isoflavones and protein during the processing of SPI and SPC (acid and alcohol leach. The majority of isoflavones present in the raw material is lost in the supernatants (90% for SPC treated with alcohol, 52% for SPI and 47% for SPC treated with acid. Total concentration of isoflavones was 652µg/g for SPI, 838µg/g for SPC (acid leach, and only 147µg/g for SPC (alcohol leach. There were no changes in the

  12. Protrombina mutante em indivíduos sob investigação de trombofilia Mutant prothrombin in individuals under thrombophilia investigation

    Directory of Open Access Journals (Sweden)

    Catarina Paula da Silva Ramos

    2008-04-01

    coagulation factor II, prothrombin G20210A (PTCR, constitutes the second most prevalent genetic defect associated with the predisposition to thrombosis or thrombophilia. In Brazil, the study of this risk factor is relatively recent and there is little available data in medical literature. OBJECTIVE: The aim of this study was to determine the frequency of PTCR in 285 individuals being investigated for thrombophilia at Fundação de Hematologia e Hemoterapia de Pernambuco (HEMOPE/PE. MATERIAL AND METHOD: The molecular biology technique used was restriction enzyme/polymerase chain reaction (RE/PCR, using specific primers and the Hind III enzyme. RESULTS: The frequency of PTCR was 6% in heterozygosis. The presence of the mutation was similar among individuals under and over 45 years old. DISCUSSION: The presence of PTCR may have been a relevant factor for the episodes of thrombosis, and the low median age of the group suggests that other genetic causes of thrombophilia must be investigated inasmuch as most publications associate the presence of genetic risk factor with thrombotic events in individuals under 45 years old. CONCLUSIONS: Our findings showed that the frequency of PTCR in the studied population is similar to the results published in medical literature for selected patients with thromboembolism and they confirm the importance of molecular testing at different age groups.

  13. Rehabilitación protésica con sobredentadura en una longeva

    Directory of Open Access Journals (Sweden)

    Marcia Hortensia Corona Carpio

    2010-12-01

    Full Text Available Se realizó un estudio donde se presenta un caso de una paciente longeva, femenina de 95 años de edad, de raza blanca, con buen estado de salud físico y mental, desdentada total superior y parcial inferior I clase de Kennedy, con experiencia protésica superior de 50 años y preocupación por rehabilitar su estética dental e interés por mantener sus dientes presentes. Se decidió realizar tratamiento con sobredentadura en los pilares presentes, se exponen técnicas y resultados obtenidos y con una evolución satisfactoria por parte de la paciente con el tratamiento alternativo realizado.

  14. Protótipo de biofiltro percolador com uso de meio suporte sintético

    OpenAIRE

    Natália Cedran Bergamini

    2014-01-01

    Resumo: Este trabalho consistiu em inovação tecnológica antes nunca testada desde a concepção, projeto, construção e operação de um biofiltro percolador de tratamento de esgoto sanitário em escala piloto. O reator foi desenvolvido nas instalações do Laboratório de Protótipos da Faculdade de Engenharia Civil da UNICAMP, cidade de Campinas, estado de São Paulo, é constituído de pré-filtro fabricado com a manta acrílica resinada, 3 reatores biológicos percoladores dispostos em séries, revestidos...

  15. Qualidade protéica de multimisturas distribuídas em Alfenas, Minas Gerais, Brasil

    OpenAIRE

    de Souza, Jane Cristina; Mauro,Ana Karina; Carvalho,Helenice Aparecida de; Márcia Regina Pereira MONTEIRO; Martino,Hércia Stampini Duarte

    2006-01-01

    OBJETIVOS: O propósito do estudo foi avaliar a qualidade protéica de quatro multimisturas distribuídas para combater a desnutrição em comunidades do Município de Alfenas-MG. MÉTODOS: Foram utilizados ratos da raça Wistar, machos, distribuídos ao acaso em 6 grupos (n=6) em gaiolas individuais de aço inox, com temperatura controlada (25ºC, desvio padrão= 2ºC), durante 14 dias. O grupo padrão recebeu dieta à base de caseína, o grupo controle dieta livre de nitrogênio e os outros quatro grupos ex...

  16. Source Code Plagiarism Detection Method Using Protégé Built Ontologies

    Directory of Open Access Journals (Sweden)

    Ion SMEUREANU

    2013-01-01

    Full Text Available Software plagiarism is a growing and serious problem that affects computer science universities in particular and the quality of education in general. More and more students tend to copy their thesis’s software from older theses or internet databases. Checking source codes manually, to detect if they are similar or the same, is a laborious and time consuming job, maybe even impossible due to existence of large digital repositories. Ontology is a way of describing a document’s semantic, so it can be easily used for source code files too. OWL Web Ontology Language could find its applicability in describing both vocabulary and taxonomy of a programming language source code. SPARQL is a query language based on SQL that extracts saved or deducted information from ontologies. Our paper proposes a source code plagiarism detection method, based on ontologies created using Protégé editor, which can be applied in scanning students' theses' software source code.

  17. ProtEx: a novel technology to display exogenous proteins on the cell surface for immunomodulation.

    Science.gov (United States)

    Singh, Narendra P; Yolcu, Esma S; Askenasy, Nadir; Shirwan, Haval

    2005-11-01

    Gene therapy as an immunomodulatory approach has the potential to treat various inherited and acquired immune-based human diseases. However, its clinical application has several challenges, varying from the efficiency of gene transfer, control of gene expression, cell and tissue targeting, and safety concerns associated with the introduction of exogenous DNA into cells/tissues. Gene therapy is also a time- and labor-intensive procedure. As an alternative, we recently developed a novel technology, ProtEx, that allows for rapid, efficient, and durable display of exogenous proteins on the surface of cells, tissues, and organs without detectable toxicity. This technology exploits the strong binding affinity (Kd = 10(-15) M) of streptavidin with biotin and involves generation of chimeric molecules composed of the extracellular portions of immunological proteins of interest and a modified form of streptavidin, biotinylation of biological surfaces, and decoration of the modified surface with chimeric proteins. Biotin persists on the cell surface for weeks both in vitro and in vivo, thereby providing a platform to display exogenous proteins with extended cell surface kinetics. Two chimeric proteins, rat FasL (SA-FasL) and human CD80 (CD80-SA), were generated and tested for cell surface display and immunomodulatory functions. SA-FasL and CD80-SA molecules persisted on the surface of various cell types for extended periods, varying from days to weeks in vitro and in vivo. The cell surface kinetics, however, were protein and cell type dependent. SA-FasL showed potent apoptotic activity against Fas+ cells as a soluble protein or displayed on the cell surface and effectively blocked alloreactive responses. The display of CD80-SA on the surface of tumor cells, however, converted them into antigen-presenting cells for effective stimulation of autologous and allogeneic T-cell responses. ProtEx technology, therefore, represents a practical and effective alternative to DNA

  18. Cirugía biológica pre protésica en artrosis temprana de rodilla

    OpenAIRE

    Wainer E. Mauricio, DR

    2014-01-01

    La artrosis (OA) de rodilla es una condición progresiva, incapacitante en distintos grados en sus etapas, terminando en el colapso y destrucción articular que requiere de cirugía protésica en las etapas más avanzadas. La cirugía biológica pre-protésica, concepto que hoy en día se implementa en el manejo de las etapas precoces de la OA, se basa en el diagnóstico precoz de esta condición y de los factores de riesgo que aseguran el progreso a estados más avanzados. Su objetivo es prolongar la vi...

  19. Development, implementation and evaluation of a peer review of teaching (PRoT) initiative in nursing education.

    Science.gov (United States)

    Mager, Diana R; Kazer, Meredith W; Conelius, Jaclyn; Shea, Joyce; Lippman, Doris T; Torosyan, Roben; Nantz, Kathryn

    2014-06-03

    For many years, an area of research in higher education has been emerging around the development and implementation of fair and effective peer evaluation programs. Recently, a new body of knowledge has developed regarding the development and implementation of fair and effective peer evaluation programs resulting in formative and summative evaluations. The purpose of this article is to describe the development, implementation, and evaluation of a peer review of teaching (PRoT) program for nursing faculty, initiated at one small comprehensive university in the northeastern United States. Pairs of nursing faculty evaluated each other's teaching, syllabi, and course materials after collaborating in a pre-evaluation conference to discuss goals of the classroom visit. Qualitative data gathered in post project focus groups revealed that faculty found their modified PRoT process to be a mutually beneficial experience that was more useful, flexible and collegial, and less stressful than their previous evaluation process.

  20. Protéza hornej končatiny a jej vliv vplyv na psychický stav pacienta

    OpenAIRE

    2015-01-01

    Bakalárska práca sa zaoberá tvorbou myoelektrickej protézy hornej končatiny a jej vplyvom na psychický stav pacienta. Vznikla za spolupráce s firmou OTTOBOCK SLOVAKIA s.r.o, kde som túto bakalársku prácu konzultovala. Z firmou ponúkaných výrobkov som si vybrala Greifer, ktorého redesign som sa rozhodla navrhnúť. Je to doplnková protéza, určená jednak na jemnú prácu s malými predmetmi, jednak na ťažšiu prácu vyžadujúcu pevný úchop. Navrhovala som s ohľadom na ergonomické, funkčné a estetické p...

  1. BlastX Result : Swiss-Prot - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available ss-Prot (release 56.9) database. The alignment information of each blast-hit is p...tabase. Link to Uniprot is provided in TogoDB. Align Length The alignment length between the above hit seque...nce and an Adiantum capillus-veneris EST sequence Bit score Score (bit) of the alignment between the above h...it sequence and an Adiantum capillus-veneris EST sequence E-value E-value of the alignment

  2. Studies of adsorption conditions in prothrombin complex concehtrates production%凝血酶原复合物制备过程吸附条件的研究

    Institute of Scientific and Technical Information of China (English)

    曹海军; 杜晞; 叶生亮; 张学俊; 王宗奎; 林方昭; 李长清

    2012-01-01

    Objective To study adsorption conditions of coagulation factor Ⅱ , Ⅵ, Ⅸ, Ⅹ in prothrombin complex concentrates (PCC) production and improve activity recovery of PCC. Methods Orthogonal experiment was designed for the e-quilibration system of DEAE-SephadexA-50 geL Three variable factors,sodium citrate,sodium chloride and pH,and their respective three levels were selected,and then equilibration buffer were prepared based on L9(33)table. 9 equal parts of the gel were equilibrated respectively by equilibration buffer, and then different equilibrated DEAE-SephadexA-50 gel was mixed with cryoprecipitate supernatant in the proportion of 1. 67g: 1L for adsorption of coagulation factor Ⅱ , Ⅵ, Ⅸ, Ⅹ, at 4℃. After that, the gel was washed, and then the coagulation factor Ⅱ , Ⅵ, Ⅸ, Ⅹ were eluted. The activity and their recovery of the coagulation factors in the eluate was measured and analyzed on the basis of orthogonal design-direct method. According to analysis results,the better adsorption conditions were obtained,and then were verified. Furthermore,the relationship between conductivity of equilibration system and activity recovery of four kinds coagulation factors was analyzed. Results Sodium citrate,sodium chloride and pH have diverse impacts on activity recovery of four coagulation factors. The effect of pH on four factors shows same tendency,the lower pH the higher recovery,on the contrary,sodium citrate and sodium chloride have distinct effects on four factors. The higher sodium citrate concentration the higher activity recovery of factors Ⅱ , Ⅵ, Ⅸ, Ⅹ,however the recovery of factor Ⅸ is higher when sodium citrate is in (0. 012 ~0. 02)mol/L The lower sodium chloride concentration the higher recovery of factorsⅡ , Ⅵ, Ⅸ, Ⅹ,however the recovery offactor X is higher when sodium chloride is in (0. 1~0.14)mol/L It was not line-relation that between conductivity of equilibration system and activity recovery of four kinds

  3. Thrombin generation, ProC(®)Global, prothrombin time and activated partial thromboplastin time in thawed plasma stored for seven days and after methylene blue/light pathogen inactivation.

    Science.gov (United States)

    Thiele, Thomas; Hron, Gregor; Kellner, Sarah; Wasner, Christina; Westphal, Antje; Warkentin, Theodore E; Greinacher, Andreas; Selleng, Kathleen

    2016-01-01

    Methylene blue pathogen inactivation and storage of thawed plasma both lead to changes in the activity of several clotting factors. We investigated how this translates into a global loss of thrombin generation potential and alterations in the protein C pathway. Fifty apheresis plasma samples were thawed and each divided into three subunits. One subunit was stored for 7 days at 4 °C, one was stored for 7 days at 22 °C and one was stored at 4 °C after methylene blue/light treatment. Thrombin generation parameters, ProC(®)Global-NR, prothrombin time and activated partial thromboplastin time were assessed on days 0 and 7. The velocity of thrombin generation increased significantly after methylene blue treatment (increased thrombin generation rate; time to peak decreased) and decreased after storage (decreased thrombin generation rate and peak thrombin; increased lag time and time to peak). The endogenous thrombin generation potential remained stable after methylene blue treatment and storage at 4 °C. Methylene blue treatment and 7 days of storage at 4 °C activated the protein C pathway, whereas storage at room temperature and storage after methylene blue treatment decreased the functional capacity of the protein C pathway. Prothrombin time and activated partial thromboplastin time showed only modest alterations. The global clotting capacity of thawed plasma is maintained at 4 °C for 7 days and directly after methylene blue treatment of thawed plasma. Thrombin generation and ProC(®)Global are useful tools for investigating the impact of pathogen inactivation and storage on the clotting capacity of therapeutic plasma preparations.

  4. Prothrombin polymorphism A19911G, factor V HR2 haplotype A4070G, and plasminogen activator-inhibitor-1 polymorphism 4G/5G and the risk of retinal vein occlusion.

    Science.gov (United States)

    Kuhli-Hattenbach, Claudia; Hellstern, Peter; Nägler, Dorit Karin; Kohnen, Thomas; Hattenbach, Lars-Olof

    2017-01-13

    Thus far, no data has become available to evaluate systematically the prevalences of prothrombin polymorphism A19911G (PT A19911G), factor V HR2 haplotype A4070G (FV A4070G), or plasminogen activator-inhibitor-1 polymorphism 4G/5G (PAI-1 4G/5G) in patients who develop retinal vein occlusion (RVO) without cardiovascular risk factors. We retrospectively evaluated comprehensive thrombophilia data from 42 preselected RVO patients without cardiovascular risk factors. The prevalences of different gene mutations and polymorphisms including factor V Leiden mutation G1691A (FVL), FV A4070G, prothrombin mutation G20210A, PT A19911G, and PAI-1 4G/5G were compared with 241 healthy controls matched for age and sex. A total of 20 patients (47.7%) were found to carry thrombophilic gene polymorphisms including FVL, FV A4070G, and homozygous PT A19911G compared with 72 of 241 controls (29.9%; p = 0.03). Subgroup analysis of patients with a significant personal or family history of thromboembolism revealed a high prevalence of FVL, FV A4070G, and homozygous PT A19911G (p = 0.005). FV A4070G was found to be significantly associated with at least two other heterozygous or one homozygous gene polymorphisms (p = 0.02). Multivariate analysis revealed the presence of FVL (p = 0.0017) and homozygous PT A19911G (p = 0.03) polymorphism as independent risk factors for the development of RVO. Our results indicate that in selected RVO patients screening for thrombophilic gene polymorphisms including FVL, FV A4070G and homozygous PT G19911A may be helpful in a high percentage of cases. Our findings suggest that hereditary thrombophilia associated with RVO is more likely to be multigenic than caused by any single risk factor.

  5. Thrombophilic Genetic Factors PAI-1, MTHFRC677T, V Leiden 506Q, and Prothrombin 20210A in Noncirrhotic Portal Vein Thrombosis and Budd-Chiari Syndrome in a Caucasian Population

    Directory of Open Access Journals (Sweden)

    Mario D’Amico

    2013-01-01

    Full Text Available Thrombophilic genetic factors PAI-1, MTHFRC677T, V Leiden 506Q, and Prothrombin 20210A were studied as risk factors in 235 Caucasian subjects: 85 patients with abdominal thrombosis (54 with portal vein thrombosis (PVT and 31 with Budd-Chiari syndrome (BCS without liver cirrhosis or hepatocellular carcinoma and 150 blood bank donors. Seventy-five patients with PVT/BCS showed associated disease or particular clinical status (46 PVT/29 BCS: 37 myeloproliferative neoplasm (20 PVT/17 BCS, 12 abdominal surgery (10 PVT/2 BCS, 10 contraception or pregnancy (6 PVT/4 BCS, 7 abdominal acute disease (6 PVT/1 BCS, and 9 chronic disease (4 PVT/5 BCS; ten patients did not present any association (8 PVT/2 BCS. PAI-14G-4G, MTHFR677TT, and V Leiden 506Q were significantly frequent (OR 95% CI and χ2 test with P value in abdominal thrombosis; in these patients PAI-14G-4G and MTHFR677TT distributions deviated from that expected from a population in the Hardy-Weinberg equilibrium (PAI-1: χ2=13.8, P<0.001; MTHFR677: χ2=7.1, P<0.01, whereas the equilibrium was respected in healthy controls. V Leiden Q506 and Prothrombin 20210A were in the Hardy-Weinberg equilibrium both in patients with abdominal thrombosis and healthy controls. Our study shows an important role of PAI-14G-4G and MTHFR677TT in abdominal thrombosis without liver cirrhosis or hepatocellular carcinoma.

  6. Comparing prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins glypican-3, Alpha feto protein and carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients.

    Science.gov (United States)

    Abd El Gawad, Iman A; Mossallam, Ghada I; Radwan, Noha H; Elzawahry, Heba M; Elhifnawy, Niveen M

    2014-06-01

    Hepatocellular carcinoma (HCC) is usually asymptomatic in the early stage and does not show elevated alpha-feto protein (AFP). AFP shows 60-80% sensitivity in diagnosing HCC. Glypican3 (GPC-3) is an oncofetal protein that is only detected in HCC cells but not in benign liver tissues, while Carcinoembryonic antigen (CEA) is expressed in various neoplasms including HCC. Although, it is not specific for HCC. Prothrombin induced by vitamin K absence-II (PIVKA-II) is an abnormal prothrombin protein that is increased in the serum of HCC patients. It has higher sensitivity and specificity compared to AFP. The aim of this study is to compare the clinical utility of PIVKA-II with GPC-3, AFP and CEA in diagnosing HCC. This study included 40 patients with HCC, 10 patients with cirrhosis as a benign control group, and 10 apparently healthy volunteers as normal controls. Serum samples were subjected to routine laboratory investigations, measurement of CEA, AFP using MEIA technique (Axsym), glypican3, and PIVKA-II using ELISA technique in the sera of all patients and controls. All markers showed the highest results in the HCC group. Higher concentrations of PIVKA-II were detected in patients with splenomegaly, and in tumors with size (>3cm). Combination of Glypican-3 and PIVKA-II showed the highest sensitivity, while GPC-3 alone and combination of GPC-3 and AFP showed the highest specificity to differentiate HCC from liver cirrhosis and normal controls. GPC-3, PIVKAII, and combination of both showed the highest sensitivity, while GPC-3 alone showed the highest specificity to differentiate HCC from liver cirrhosis. Glypican-3 is the only oncofetal antigen that showed comparable high diagnostic accuracy as PIVKA-II in diagnosing HCC among Egyptian patients. Copyright © 2014. Production and hosting by Elsevier B.V.

  7. Diffusion incohérente des neutrons : modèles analytiques pour la dynamique interne des protéines

    Science.gov (United States)

    Bicout, D. J.

    2005-11-01

    La dynamique interne des protéines joue un rôle central dans la stabilité, la fonction et l'activité biologique de ces biomolécules. Il est maintenant établi que les fluctuations d'états conformationnels des protéines influencent fortement la plupart des réactions biochimiques et s'accompagnent d'une augmentation brutale des déplacements carrés moyens des atomes au dessus de la température de la transition dynamique. Dans cette contribution, nous présentons une revue critique de quelques modèles théoriques couramment utilisés dans la littérature pour l'analyse des mouvements internes des protéines et la description de la transition dynamique.

  8. Aplicação de zamac-8 em molde-protótipo usinado e análise do moldado em polipropileno

    OpenAIRE

    Gryzinski, Ismael

    2013-01-01

    A razão da existência de um molde-protótipo é de se obter rapidamente um injetado com o mesmo material e utilizando o mesmo processo de fabricação do produto final, com baixo custo. Seus injetados podem servir como protótipos, lotes pilotos, pré-séries ou ainda cobrirem a necessidade de uma baixa demanda de produto ou de pequenos lotes. Critérios de manufaturabilidade e de qualidades mecânicas são importantes na fabricação do molde-protótipo. Entretanto, além destes critérios, este molde deve...

  9. Hernia incisional grande: Resultado del tratamiento quirúrgico con material protésico

    Directory of Open Access Journals (Sweden)

    Juan Francisco Puerta Alvarez

    1996-12-01

    Full Text Available Se realizó un estudio observacional y prospectivo para evaluar el tratamiento quirúrgico de las grandes hernias incisionales, con material protésico en 75 pacientes operados en el Hospital Militar Docente "Joaquín Castillo Duany" de Santiago de Cuba, desde febrero de 1990 hasta julio de 1994. Se conformaron 2 grupos: de estudio con 50 pacientes (subdividido en 2 de 25 cada uno y testigo con 25. En este último la pared abdominal se cerró por planos con sutura irreabsorbible y sin material protésico, y al del estudio, en uno se practicó igual procedimiento pero utilizando malla de Mersilene y en el otro se sustituyó por duramadre homóloga. Las hernias incisionales grandes fueron más frecuentes en mujeres de 40 a 60 años con incisiones primarias medias; la mayoría de ellas obesas y diabéticas. Se comprobó que no hubo rechazo a la duramadre homóloga, grupo éste que mostró el menor índice de recidivas y complicaciones, y con los mejores resultados a los 5 años de seguimiento posoperatorioAn observation and prospective study was carried out to assess the surgical treatment of the big incisional hernias with prosthetic material in 75 operated patients in the "Joaquín Castillo Duany" Educational Military Hospital, at Santiago de Cuba, from February 1990 to July 1994. Two groups were formed: a study group with 50 patients, subdivided in two groups of 25 each, and a witness group with 25 patients. In this last group the abdominal wall was closed by planes with non reabsorbable suture and without prosthetic material, and in the study group, in one 25 patients subgroup the same procedure was carried out, but using Mersilene mesh, and in the other subgroup the mesh was substituted with homologous duramater. The big incisional hernias were more frequent in women from 40 to 60 years old with partial primary incision, mostly obese and diabetic. There was a confirmation of no reject to the homologous duramater. This group showed the smallets

  10. Levelling The Playing Field: Reducing Barriers To Mentoring For Women Protégés In The South African Organisational Context

    Directory of Open Access Journals (Sweden)

    Kevin Stone

    2005-11-01

    Full Text Available The purpose of this study was to investigate the impediments to effective mentoring faced by women protégés at two South African organisations. The purposive sampling method was used to identify female employees who had participated as protégés in a mentoring relationship (N=17. Qualitative methods were used to collect and analyse data on the respondents’ experiences of the mentoring relationship. The findings indicated numerous barriers to mentoring for women. A basic taxonomy of recommendations for reducing these barriers in the workplace is recommended.

  11. Caracterización de la trombosis protésica en el servicio de cardiología de Las Tunas. 2006-2011

    OpenAIRE

    Maikel Santos Medina; Leticia Pardo Pérez; Yudelquis Ojeda Riquenes; Raúl Celorrio Fernández; Roberto Rabert Fernández

    2015-01-01

    Se realizó un estudio descriptivo y transversal en el Servicio de Cardiología del Hospital “Dr. Ernesto Guevara de la Serna” de la provincia de Las Tunas, con el objetivo de caracterizar la trombosis de prótesis valvular en el período entre 2006 y 2011. El universo estuvo conformado por los 34 pacientes ingresados en el servicio por sospecha de trombosis protésica y la muestra por los 29 casos con diagnóstico definitivo de trombosis valvular protésica. Se utilizó la estadística descriptiva a ...

  12. Composição química de tabletes de caldo de carne: nitrogênio protéico, não-protéico e fenilalanina

    Directory of Open Access Journals (Sweden)

    Guimarães Claudia Passos

    2002-01-01

    Full Text Available A redução dos níveis sangüíneos de fenilalanina (Phe em pacientes fenilcetonúricos requer o conhecimento preciso do teor de Phe nos alimentos, para que possa haver um controle da ingestão desse aminoácido. Este trabalho teve como objetivo estudar o teor protéico e de Phe em tabletes de caldo de carne de duas marcas comerciais, contribuindo com informações para a elaboração de cardápios. A análise de aminoácidos foi realizada por cromatografia de troca iônica em autoanalisador de aminoácidos e os teores de umidade, proteína bruta (Nx6,25 e lipídeos foram determinados por métodos descritos na AOAC (1995. A fibra alimentar foi quantificada por método enzimático. O nitrogênio protéico (NP foi determinado após precipitação ácida da proteína. Observamos que os teores de umidade, lipídeos foram semelhantes nos dois produtos com valores médios de 3,7%, e 8,4%, respectivamente. Os teores de fibra foram inferiores a 2%, mas vale ressaltar o elevado teor de minerais, da ordem de 61% no produto A e de 54% no produto B. Comparando-se os valores de nitrogênio total e NP, verificamos que aproximadamente 95% do N correspondem a N de origem não protéica. O teor protéico real, pela somatória de aminoácidos, era de apenas 0,71g/100g e de 0,84g/100g nas amostras A e B, respectivamente, e não foi possível detectar a presença de Phe nestas amostras. Teoricamente, considerando que uma proteína contém 4% de Phe em sua composição, as amostras analisadas contém no máximo 34mg Phe/100g, o que corresponde a 3,6mg Phe por tablete de caldo de carne de 10,5g. Esta reduzida quantidade justifica a dificuldade em se detectar analiticamente este aminoácido. A elevada quantidade de nitrogênio não protéico corresponde à presença de monoglutamato de sódio (realçador de sabor, de modo que a conversão Nx6,25 resulta em valores protéicos superestimados.

  13. MoonProt: a database for proteins that are known to moonlight

    Science.gov (United States)

    Mani, Mathew; Chen, Chang; Amblee, Vaishak; Liu, Haipeng; Mathur, Tanu; Zwicke, Grant; Zabad, Shadi; Patel, Bansi; Thakkar, Jagravi; Jeffery, Constance J.

    2015-01-01

    Moonlighting proteins comprise a class of multifunctional proteins in which a single polypeptide chain performs multiple biochemical functions that are not due to gene fusions, multiple RNA splice variants or pleiotropic effects. The known moonlighting proteins perform a variety of diverse functions in many different cell types and species, and information about their structures and functions is scattered in many publications. We have constructed the manually curated, searchable, internet-based MoonProt Database (http://www.moonlightingproteins.org) with information about the over 200 proteins that have been experimentally verified to be moonlighting proteins. The availability of this organized information provides a more complete picture of what is currently known about moonlighting proteins. The database will also aid researchers in other fields, including determining the functions of genes identified in genome sequencing projects, interpreting data from proteomics projects and annotating protein sequence and structural databases. In addition, information about the structures and functions of moonlighting proteins can be helpful in understanding how novel protein functional sites evolved on an ancient protein scaffold, which can also help in the design of proteins with novel functions. PMID:25324305

  14. Analysis of gene and protein name synonyms in Entrez Gene and UniProtKB resources

    KAUST Repository

    Arkasosy, Basil

    2013-05-11

    Ambiguity in texts is a well-known problem: words can carry several meanings, and hence, can be read and interpreted differently. This is also true in the biological literature; names of biological concepts, such as genes and proteins, might be ambiguous, referring in some cases to more than one gene or one protein, or in others, to both genes and proteins at the same time. Public biological databases give a very useful insight about genes and proteins information, including their names. In this study, we made a thorough analysis of the nomenclatures of genes and proteins in two data sources and for six different species. We developed an automated process that parses, extracts, processes and stores information available in two major biological databases: Entrez Gene and UniProtKB. We analysed gene and protein synonyms, their types, frequencies, and the ambiguities within a species, in between data sources and cross-species. We found that at least 40% of the cross-species ambiguities are caused by names that are already ambiguous within the species. Our study shows that from the six species we analysed (Homo Sapiens, Mus Musculus, Arabidopsis Thaliana, Oryza Sativa, Bacillus Subtilis and Pseudomonas Fluorescens), rice (Oriza Sativa) has the best naming model in Entrez Gene database, with low ambiguities between data sources and cross-species.

  15. Hacking interdit découvrez les techniques des hackers pour mieux vous protéger

    CERN Document Server

    Gomez-Urbina, Alexandre

    2010-01-01

    Aujourd'hui, impossible de surfer sur Internet sans protection efficace ! Mais même avec des logiciels appropriés, nul n'est à l'abri du danger ! Virus, usurpations d'adresse IP, troyens, intrusions dans votre système, attaques par phishing : l'imagination des pirates est sans limite ! La seule solution est de vous protéger efficacement et pour cela, un moyen : connaître les techniques des hackers pour mieux déjouer leurs pièges ! Un internaute averti en vaut deux !

  16. Hipertexto : um protótipo implementado em Zim, para suporte da análise de requisitos

    OpenAIRE

    Guilherme Martim Doege

    1991-01-01

    Neste trabalho se descreve a implementação de um protótipo de sistema de hipertexto, construído sobre o sistema de banco de dados ZIM, para uso na análise de requisitos de sistemas de informações de escritórios. Inicialmente, são apresentadas as principais características dos sistemas de hipertexto, destacando-se o hibridismo entre banco de dados, esquemas de representação e interfaces. Comenta-se a funcionalidade, aplicações, vantagens e desvantagens de hipertextos. A seguir, são mencionados...

  17. Estratégias para redução protéica de dietas para frangos de corte

    OpenAIRE

    2010-01-01

    A presente tese foi realizada com o objetivo de estudar as relações entre valina (VAL) e isoleucina (ILE), determinar as exigências de valina e a razão pela qual dietas de baixo conteúdo protéico tendem a causar queda no desempenho de frangos de corte. Desta forma, três experimentos foram conduzidos utilizando dietas a base de milho e farelo de soja e linhagens de frangos de corte comercialmente disponíveis. No primeiro estudo foram avaliadas deficiências de VAL e ILE em dietas práticas para ...

  18. PAI-1 4G-4G, MTHFR 677TT, V Leiden 506Q, and Prothrombin 20210A in Splanchnic Vein Thrombosis: Analysis of Individual Patient Data From Three Prospective Studies.

    Science.gov (United States)

    Pasta, Linda; Pasta, Francesca; D'Amico, Mario

    2016-03-01

    There are no univocal opinions on the role of genetic thrombophilia on splanchnic vein thrombosis (SVT). We defined genetic thrombophilia the presence of one of these thrombophilic genetic factors (THRGFs): PAI-1 4G-4G, MTHFR 677TT, V Leiden 506Q, and prothrombin 20210A. To evaluate the frequencies of these THRGFs in SVT patients, we analyzed individual data of 482 Caucasian patients, recruited from 2000 to 2014 in three prospective studies. SVT was defined as the presence of thrombosis of portal (PVT), mesenteric (MVT), splenic (SPVT), cava (CT), and hepatic vein (Budd Chiari syndrome, BCS). Pre-hepatic SVT (pre-HSVT) was defined as PVT with or without MVT/SPVT, without BCS. Post-hepatic SVT (post-HSVT) was BCS with or without PVT/MVT/SPVT. We compared 350 patients with liver cirrhosis (LC), 47 hepatocellular carcinoma (HCC), 37 myeloproliferative neoplasm (MPN), 38 associated disease (AD), 10 without any associated disease (WAD), vs 150 healthy controls (HC); 437 patients showed pre-HSVT and 45 post-HSVT. Thrombophilia was present in 294/482 (60.9%) patients: 189/350 LC (54.0%), 31/47 (66.0%) HCC, 29/39 (74.4%) MPN, 35/38 AD (92.1%), and 10/10 (100%) WAD, and 54/150 (36.0%) in HC. In the total group, we found 175 PAI-1 4G-4G, 130 MTHFR 677TT, 42V Leiden 506Q, and 27 prothrombin 20210A; 75 patients showed presence of >1 TRHGF; the more frequent association was PAI-1 4G-4G/MTHFR 677TT, in 36 patients. PAI-1 4G-4G and MTHFR 677TT were significantly more frequent in patients with SVT (P values 4G-4G and MTHFR 677TT distributions deviated significantly from that expected from a population in Hardy-Weinberg equilibrium. Thrombophilia was significantly less frequent in patients with pre-HSVT (250/437, 57.2%) than in patients with post-HSVT (44/45, 97.8%). Our study shows the significant prevalence of PAI-1 4G-4G and MTHFR 677TT in SVT, mainly in post-HSVT.

  19. SVM-Prot 2016: A Web-Server for Machine Learning Prediction of Protein Functional Families from Sequence Irrespective of Similarity.

    Science.gov (United States)

    Li, Ying Hong; Xu, Jing Yu; Tao, Lin; Li, Xiao Feng; Li, Shuang; Zeng, Xian; Chen, Shang Ying; Zhang, Peng; Qin, Chu; Zhang, Cheng; Chen, Zhe; Zhu, Feng; Chen, Yu Zong

    2016-01-01

    Knowledge of protein function is important for biological, medical and therapeutic studies, but many proteins are still unknown in function. There is a need for more improved functional prediction methods. Our SVM-Prot web-server employed a machine learning method for predicting protein functional families from protein sequences irrespective of similarity, which complemented those similarity-based and other methods in predicting diverse classes of proteins including the distantly-related proteins and homologous proteins of different functions. Since its publication in 2003, we made major improvements to SVM-Prot with (1) expanded coverage from 54 to 192 functional families, (2) more diverse protein descriptors protein representation, (3) improved predictive performances due to the use of more enriched training datasets and more variety of protein descriptors, (4) newly integrated BLAST analysis option for assessing proteins in the SVM-Prot predicted functional families that were similar in sequence to a query protein, and (5) newly added batch submission option for supporting the classification of multiple proteins. Moreover, 2 more machine learning approaches, K nearest neighbor and probabilistic neural networks, were added for facilitating collective assessment of protein functions by multiple methods. SVM-Prot can be accessed at http://bidd2.nus.edu.sg/cgi-bin/svmprot/svmprot.cgi.

  20. 13 CFR 126.618 - How does a HUBZone SBC's participation in a Mentor-Protégé relationship affect its participation...

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false How does a HUBZone SBC's... Assistance § 126.618 How does a HUBZone SBC's participation in a Mentor-Protégé relationship affect its... HUBZone SBC qualifies as small under part 121 of this chapter, SBA will not find affiliation between...

  1. 48 CFR 653.219-71 - DOS form DS-4053, Department of State Mentor-Protégé Program Application.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false DOS form DS-4053... Regulations System DEPARTMENT OF STATE CLAUSES AND FORMS FORMS Prescription of Forms 653.219-71 DOS form DS-4053, Department of State Mentor-Protégé Program Application. As prescribed in 619.102-70(i), DS-4053...

  2. Qualidade protéica de multimisturas distribuídas em Alfenas, Minas Gerais, Brasil Protein quality from "multimixture" distributed in Alfenas, Minas Gerais, Brazil

    Directory of Open Access Journals (Sweden)

    Jane Cristina de Souza

    2006-12-01

    Full Text Available OBJETIVOS: O propósito do estudo foi avaliar a qualidade protéica de quatro multimisturas distribuídas para combater a desnutrição em comunidades do Município de Alfenas-MG. MÉTODOS: Foram utilizados ratos da raça Wistar, machos, distribuídos ao acaso em 6 grupos (n=6 em gaiolas individuais de aço inox, com temperatura controlada (25ºC, desvio padrão= 2ºC, durante 14 dias. O grupo padrão recebeu dieta à base de caseína, o grupo controle dieta livre de nitrogênio e os outros quatro grupos experimentais receberam dietas à base de multimistura. Para avaliação da qualidade protéica foram utilizados os métodos biológicos Quociente de Eficiência Protéica, Razão Protéica Líquida, Utilização Protéica Líquida e Digestibilidade Verdadeira. RESULTADOS: O quociente de eficiência protéica, a razão protéica líquida e a digestibilidade dos grupos recebendo multimistura foram inferiores (pOBJECTIVE: The study was developed to evaluate the protein quality of four multimixtures distributed in communities in Alfenas, MG, Brazil, to combat malnutrition. METHODS: Male Wistar rats were randomaly assigned to one of six groups (n=6 and individually housed in stainless steel cages on controlled temperature (25ºC, standard deviation 2ºC for 14 days. The standard group was fed a casein diet, control group was fed a free nitrogen diet and four-test groups were fed different multimixture diets. A biological assay was carried out to determine the protein quality by Protein Efficiency Ratio, Net Protein Ratio, Net Protein Utilization and True Protein Digestibility. RESULTS: The Multimixture test groups showed lower (p<0.05 Protein Efficiency Ratio, Net Protein Ratio and digestibility than casein group. The net retention protein from multimixture test groups was lower (p<0.05 than casein group, except the group fed with multimixture contained a higher powdered milk proportion and was not added with powdered seeds, leaf cassava and egg

  3. Avaliação da determinação do tempo de protrombina em amostras de sangue colhidas por duas diferentes técnicas Evaluation of determination of prothrombin time in blood samples using two different collection techniques

    Directory of Open Access Journals (Sweden)

    Paulo Roberto de Melo Reis

    2005-08-01

    Full Text Available O presente trabalho tem como objetivo avaliar a interferência de diferentes técnicas de coleta sanguínea na determinação do tempo de protrombina. Duas técnicas de coleta foram utilizadas: uma, realizada pelo sistema a vácuo; a outra, realizada mediante aspiração manual com seringa de plástico. Os valores obtidos na determinação do tempo de protrombina provenientes das duas técnicas de coleta foram comparados. Foram analisadas amostras de pacientes que apresentavam tempo de protrombina normais e anormais por estarem em tratamento oral com medicação anticoagulante. A conclusão foi que o processo de coleta utilizando tanto o sistema a vácuo como a aspiração manual com seringa de plástico não interfere no resultado do teste, e os valores obtidos, comparando-se os dois métodos de coleta, não apresentaram diferenças estatisticamente significativas.The objective of the present study is to evaluate the interference of different blood collection techniques in determining prothrombin time. Two collection techniques were used. One of them uses the vacuum system and the other, manual aspiration using plastic syringe. The obtained values of determination of prothrombin time from these two techniques were compared. We analyzed patients’ samples that presented normal and non-normal prothrombin time owing to oral treatment with anticoagulant medication. The conclusion was that the collection processes using either vacuum system or manual aspiration with plastic syringe to obtain samples destined to the prothrombin time analysis do not interfere in the test result and the obtained values. In comparing both collecting techniques we did not find any significant statistical differences.

  4. Nutrition et santé : lipides et protéines d’origine végétale - De nouvelles données pour juger de la qualité des protéines végétales chez l’homme - Implications et perspectives

    Directory of Open Access Journals (Sweden)

    Mariotti François

    2003-01-01

    Full Text Available La qualité des protéines pour l’alimentation de l’homme est un sujet très ancien mais les différentes méthodes proposées jusqu’ici pour évaluer la qualité protéique ont été largement critiquées, pour des problèmes conceptuels et méthodologiques. Depuis quelques années des avancées ont pu être faites grâce à l’emploi d’isotopes stables dans la phase postprandiale chez l’homme. Ces techniques proposent des mesures fines et conceptuellement adaptées pour évaluer la qualité des protéines et explorer le concept de qualité protéique. Ces approches ont montré que les différences globales de qualités entre sources protéiques chez l’homme au bon statut nutritionnel sont bien plus faibles que cela n’était attendu. Parallèlement, la nature des protéines alimentaires pourrait avoir des effets métaboliques plus subtils, au niveau de certains tissus ou au niveau des voies métaboliques secondaires de certains acides aminés, dont il faudra mesurer l’étendue et évaluer les conséquences physiologiques et fonctionnelles.

  5. Endothelial Nitric Oxide Synthase T-786C Mutation, Prothrombin Gene Mutation (G-20210-A and Protein S Deficiency Could Lead to Myocardial Infarction in a Very Young Male Adult

    Directory of Open Access Journals (Sweden)

    Milka Klincheva

    2016-01-01

    Full Text Available INTRODUCTION: Myocardial infarction is a rare medical event in young people. The main reasons include congenital coronary abnormalities, coronary artery spasm, and coronary thrombosis due to hypercoagulable states (hereditary and acquired. AIM: We present a case of a young male adult with myocardial infarction caused by a combination of gene mutations and anticoagulation protein deficiency. CASE PRESENTATION: A 19 years old young man was admitted to our hospital complaining of chest pain during the last two weeks. The patient did not have any known cardiovascular risk factors, except a positive family anamnesis. Subacute inferior nonST segment myocardial infarction was diagnosed according to the patient’s history, electrocardiographic and laboratory findings. Coronary angiography revealed suboclusive thrombus in the proximal, medial and distal part of the right coronary artery (TIMI 2. Percutaneous coronary intervention was performed. Anticoagulant and antiagregant therapy (heparin, acetilsalicilic acid and clopidogrel according to protocol was started. The hospital stay was uneventful. Homozygous endothelial nitric oxid synthase (eNOS T-786-C mutation, heterozygote prothrombin gene mutation (G-20210-A, and protein S deficiency were verified from the thrombophilia testing. Other trombophilic tests were normal. Three months after discharge from hospital another coronary angiography was performed. It revealed normal coronary arteries. Four years after the attack, the patient is free of symptoms and another cardiovascular event. CONCLUSION: Combination of genetic mutations and anticoagulation protein deficiency could be a reasonable cause for myocardial infarction in a very young male adult without any other cardiovascular risk factors.

  6. 凝血酶原前体蛋白在华法林抗凝效果监测中的应用%Application of Precursor of Prothrombin in Monitoring of Anticoagulation Effect for Warfarin

    Institute of Scientific and Technical Information of China (English)

    李伟皓; 李彦会; 李伟; 张会丰; M.J.Shearer; 刘永春

    2003-01-01

    @@ 华法林是一种常用口服抗凝剂,具有服用方便、疗效确切等优点,被广泛用于临床.目前,临床多以凝血酶原时间(PT)、国际标准化比率(INR)、凝血酶原片段1+2(F1+2)等指标对华法林抗凝效果进行评价.凝血酶原前体蛋白(Prescursor of Prothrombin.Proteins Induced by Vitamin K Abense of Antagonism,PIVKA-Ⅱ)作为反映机体维生素K营养状况最敏感的指标[1],其作用已被医学界公认,但其对评价华法林抗凝效果是否具有应用价值,目前国内尚未见报道.为此我们进行了试验研究,报道如下.

  7. Protágoras e a doxografia platônica sobre o mais eminente sofista : estudo e tradução = Protagora and the platonic doxography about the most eminente sophist : study and translation

    OpenAIRE

    Osvaldo Cunha Neto

    2012-01-01

    Resumo: O diálogo Protágoras coloca frente a frente Sócrates e Protágoras...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital. Abstract: The dialogue Protagoras puts face to face Socrates and Protagoras...Note: The complete abstract is available with the full electronic document.

  8. Developing an University Ontology in Education Domain using Protégé for Semantic Web

    Directory of Open Access Journals (Sweden)

    SANJAY KUMAR MALIK,

    2010-09-01

    Full Text Available The current web is based on HTML which is not able to be exploited by information retrieval techniques and hence processing of information on web is mostly restricted to manual keyword searches which results in irrelevant information retrieval . This limitation may be overcome by a new web architecture known as semantic web which is an intelligent and meaningful web proposed by Sir Tim Berner’s Lee. In his roadmap for semantic web, Ontology plays a pivotal role in information exchange, use and re-use knowledge, shared and common understanding of a domain that can be communicated between people and across application systems which is the goal of semantic web. Ontology is used to capture knowledge about any domain of interest with the objective of incorporating the machineunderstandable data on the current human-readable web. Web Ontology Language (OWL is a semantic markup language for sharing ontologies on the web and is designed for use by software agents to empower them to comprehend the meaning of web documents. Ontology is a broad term including a wide range of activities,complexities and issues in which Ontology Development is one of the most fundamental and significant concern. There may be various methodologies or tools for ontology development . In this paper, we consider the education domain and demonstrate thedevelopment of an University Ontology using Protégé 3.4 Editor. Indraprastha University, Delhi, India has been taken as an example for the Ontology Development and various aspects like super class and sub class hierarchy, creating a sub class, instances for classes illustr

  9. Incumplimiento de las indicaciones postrehabilitación protésica y su influencia en la calidad de vida

    Directory of Open Access Journals (Sweden)

    Maitee Lajes Ugarte

    Full Text Available Introducción: la existencia de pacientes con prótesis totales que presentan problemas para desarrollar sus funciones puede tener diferentes causas, entre ellas el incumplimiento de las indicaciones médicas, y factores capaces de repercutir de forma directa sobre los pacientes, que pueden modificar su calidad de vida en relación con la rehabilitación protésica. Objetivo: Evaluar la influencia del incumplimiento de las indicaciones post rehabilitación protésica en la calidad de vida de los pacientes desdentados totales tratados en las Clínicas Estomatológicas Provincial Docente Ismael Clark Mascaró y La Vigía durante el período de septiembre del 2009 a septiembre del 2013. Material y método: Se realizó un estudio descriptivo en un universo de 254 pacientes desdentados totales rehabilitados. A través de un muestreo probabilístico aleatorio se obtuvo una muestra de 43 pacientes. Resultados: El nivel de incumplimiento bajo se presentó principalmente en el grupo de 50-69 años, con un 50 % y el alto en el grupo de 70 años y más. Los elementos no cumplidos por los pacientes resultaron ser: método incorrecto de sostener las prótesis para realizar su higiene (46,5 %, uso permanente de las prótesis (30,2 % e incorrecta frecuencia del cepillado de las prótesis (25,6 %. Conclusiones: Los mayores niveles de incumplimiento de las indicaciones de uso, cuidado e higiene de las prótesis totales se presentaron en el grupo de 70 años y más. La calidad de vida de los pacientes con rehabilitación protésica total está influenciada por el nivel de incumplimiento de las indicaciones de uso, cuidado e higiene de las prótesis.

  10. Pneumatic tube system transport does not alter platelet function in optical and whole blood aggregometry, prothrombin time, activated partial thromboplastin time, platelet count and fibrinogen in patients on anti-platelet drug therapy

    Science.gov (United States)

    Enko, Dietmar; Mangge, Harald; Münch, Andreas; Niedrist, Tobias; Mahla, Elisabeth; Metzler, Helfried; Prüller, Florian

    2017-01-01

    Introduction The aim of this study was to assess pneumatic tube system (PTS) alteration on platelet function by the light transmission aggregometry (LTA) and whole blood aggregometry (WBA) method, and on the results of platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen. Materials and methods Venous blood was collected into six 4.5 mL VACUETTE® 9NC coagulation sodium citrate 3.8% tubes (Greiner Bio-One International GmbH, Kremsmünster, Austria) from 49 intensive care unit (ICU) patients on dual anti-platelet therapy and immediately hand carried to the central laboratory. Blood samples were divided into 2 Groups: Group 1 samples (N = 49) underwent PTS (4 m/s) transport from the central laboratory to the distant laboratory and back to the central laboratory, whereas Group 2 samples (N = 49) were excluded from PTS forces. In both groups, LTA and WBA stimulated with collagen, adenosine-5’-diphosphate (ADP), arachidonic acid (AA) and thrombin-receptor-activated-peptide 6 (TRAP-6) as well as platelet count, PT, APTT, and fibrinogen were performed. Results No statistically significant differences were observed between blood samples with (Group 1) and without (Group 2) PTS transport (P values from 0.064 – 0.968). The AA-induced LTA (bias: 68.57%) exceeded the bias acceptance limit of ≤ 25%. Conclusions Blood sample transportation with computer controlled PTS in our hospital had no statistically significant effects on platelet aggregation determined in patients with anti-platelet therapy. Although AA induced LTA showed a significant bias, the diagnostic accuracy was not influenced. PMID:28392742

  11. The clinical study of prothrombin precursor protein in monitoring anticoagulation%凝血酶原前体蛋白在抗凝监测中的临床研究

    Institute of Scientific and Technical Information of China (English)

    朱平; 刘菁; 黄劲松; 舒峻; 周志凌; 庄建; 吴若彬; 肖学钧; 郑少忆; 陈寄梅; 卢聪; 范瑞新; 郭惠明

    2009-01-01

    目的 探讨凝血酶原前体蛋白(PIVKA-Ⅱ)在华法林抗凝治疗监测中的意义.方法 动态监测60例心脏机械瓣膜置换术后行抗凝治疗病人(实验组)的血浆PIVKA-Ⅱ浓度、凝血酶原时间(prothrombin time,PT)、国际标准化比率(international normalized ratio,INR),比较3者出现明显异常的最早时间,分析PIVKA-Ⅱ与PI'及INR之间的相关性.结果 实验组首次服华法林后6h,血浆PIVKA-Ⅱ浓度即出现明显异常,并随时间增加而增高;实验组首次服华法林后60 h,PT出现明显异常,并随时间增加而增高;实验组首次服华法林后24 h,INR出现明显异常,并随时间增加而增高;线性相关分析,结果显示PIVKA-Ⅱ浓度与PT呈正相关(r=0.786,P<0.01);PIVKA-Ⅱ浓度与INR呈正相关(r=0.737,P<0.01).结论 在抗凝治疗的患者中,通过监测患者的血浆PIVKA-Ⅱ浓度可以有效地监测其抗凝程度,联合监测患者的血浆PIVKA-Ⅱ浓度、PT、INR则更能早期、有效的监测患者抗凝程度.

  12. Comparison between Prothrombin Complex Concentrate (PCC) and Fresh Frozen Plasma (FFP) for the Urgent Reversal of Warfarin in Patients with Mechanical Heart Valves in a Tertiary Care Cardiac Center.

    Science.gov (United States)

    Fariborz Farsad, Bahram; Golpira, Reza; Najafi, Hamideh; Totonchi, Ziae; Salajegheh, Shirin; Bakhshandeh, Hooman; Hashemian, Farshad

    2015-01-01

    Fresh frozen plasma (FFP) and prothrombin complex concentrate (PCC) reverse oral anticoagulants such as Warfarin. We compared the standard dosage of FFP and PCC in terms of efficacy and safety for patients with mechanical heart valves undergoing interventional procedures while receiving Warfarin. Fifty patients were randomized (25 for each group) with mechanical heart valves [international normalized ratio (INR) >2.5]. FFP dosage was administered based on body weight (10-15 mL/Kg), while PCC dosage was administered based on both body weight and target INR. INR measurements were obtained at different time after PCC and FFP infusion. The mean ± SD of INR pre treatment was not significantly different between the PCC and FFP groups. However, over a 48-hour period following the administration of PCC and FFP, 76% of the patients in the PCC group and only 20% of the patients in the FFP group reached the INR target. Five (20%) patients in the PCC group received an additional dose of PCC, whereas 17 (68%) patients in the FFP group received a further dose of FFP (P=0.001). There was no significant difference between the two groups in Hb and Hct before and during a 48-hour period after PCC and FFP infusion. As regards safety monitoring and adverse drug reaction screening in the FFP group, the INR was high (INR > 2.5) in 86% of the patients. There was no report of hemorrhage in both groups. PCC reverses anticoagulation both effectively and safely while having the advantage of obviating the need to extra doses.

  13. Desarrollo protálico de Lygodium heterodoxum y Lygodium venustum (Schizaeaceae

    Directory of Open Access Journals (Sweden)

    Aniceto Mendoza

    1999-06-01

    Full Text Available Se describe la morfogénesis y la morfología adulta de los gametofitos de Lygodium heterodoxum Kunze y Lygodium venustum Sw. El patrón de germinación corresponde al tipo Vittaria de acuerdo con Nayar y Kaur (1971 o al tipo Lygodium en opinión de Raghavan (1989. El desarrollo protálico es tipo Adiantum con transición al tipo Ceratopteris. Los gametangios son del tipo común de los helechos leptosporangiados y el esporofito se desarrolla a los 85 días a partir de la siembra, sus hojas son flabeliformes, con tricomas en forma de clava, unicelulares, en el margen y ambas superficies de la lámina; los estomas son anomocíticos. El experimento desarrollado transfiriendo un fragmento de medio de cultivo en el que hubieron crecido gametofitos arquegoniados y gametofitos anteridiados mas peque os a una nueva caja de cultivo en la que se sembraron esporas, dió como resultado el que, a los 14 días después de la siembra crecieran gametofitos inicialmente anteridiados con tendencia a forma espatulada sin zona meristemática ni cojinete; los anteridios se mostraron normales con anterozoides activos en agua. Se demuestra de esta manera la presencia en el medio de cultivo de sustancias con actividad anteridogénica.The development of the gametophytes of Lygodium heterodoxum Kunze and Lygodium venustum Sw. is described on the basis of spores collected in the States of Veracruz and Sinaloa, México. The germination of the spores is of the Vittaria-type or Lygodium-type. Prothallial development is of the Adiantum-type with transition to Ceratopteris-type. The gametangia are the usual for leptosporangiate ferns. The first leaf of the sporophyte appeared 85 days after spore sowing; it is digitate-flabelliform, with unicellular club shaped trichomes in margin and both surfaces, and the stomata anomocytic. Unisexual gametophytes (only with antheridia or archegonia were found in the cultures, so the presence of hormonal substances with "antheridogenic

  14. Analyse des cadres juridiques des aires marines protégées des pays ouest africains

    Directory of Open Access Journals (Sweden)

    Marie Cuq

    2013-06-01

    Full Text Available En analysant de manière transversale les droits nationaux relatifs aux aires protégées de sept pays d'Afrique de l'Ouest (le Cap Vert, la Gambie, la Guinée, la Guinée-Bissau, la Mauritanie, le Sénégal et la Sierra Leone, cette étude tend à apporter des éléments de connaissances de ces droits nationaux, tant sur le plan normatif qu'institutionnel, et à s'interroger sur le contenu, la mise en oeuvre et l'adaptabilité de ceux-ci à la lumière des enjeux environnementaux, sociaux et économiques de la sous-région ouest-africaine.

  15. Creació d'un plug-in de Protégé per a podar ontologies

    OpenAIRE

    2011-01-01

    La creació d'una ontologia partint de zero és una feina llarga i feixuga que pot simplificar-se si, partint d'una ontologia més general, es poden podar les parts que no formen part del domini de context. Aquesta memòria té una doble vessant: d'un costat l'estudi de l'estat de l'art de les ontologies (història, aplicacions, línies de treball, etc) i de l'altra l'anàlisi i disseny d'un plug-in en java per a Protégé que implementi l'algorisme de poda. La creación de una ontología partiendo de...

  16. Cirugía biológica pre protésica en artrosis temprana de rodilla

    Directory of Open Access Journals (Sweden)

    Wainer E. Mauricio, DR

    2014-09-01

    Full Text Available La artrosis (OA de rodilla es una condición progresiva, incapacitante en distintos grados en sus etapas, terminando en el colapso y destrucción articular que requiere de cirugía protésica en las etapas más avanzadas. La cirugía biológica pre-protésica, concepto que hoy en día se implementa en el manejo de las etapas precoces de la OA, se basa en el diagnóstico precoz de esta condición y de los factores de riesgo que aseguran el progreso a estados más avanzados. Su objetivo es prolongar la vida útil de la articulación nativa y retardar o eliminar la necesidad de una prótesis de rodilla. Esto ha motivado innumerables estudios preclínicos y clínicos en base al uso de nuevos elementos de ingeniería de tejidos, uso de implantes de condrocitos autólogos y manejo de células madre. Los resultados en el corto y mediano plazo son auspiciosos, pero se requieren mejores niveles de evidencia científica de estos estudios y seguimientos más prolongados. En el presente artículo se analiza el concepto de la artrosis temprana, la detección de las lesiones de cartílago y los factores de riesgo de los pacientes que los llevan a una artrosis y los protocolos actuales de tratamiento biológico de Clínica Las Condes.

  17. Les sources de protéines dans l’alimentation du bétail en France : la place des oléoprotéagineux

    OpenAIRE

    Peyronnet Corinne; Lacampagne Jean-Paul; Le Cadre Patricia; Pressenda Frédéric

    2014-01-01

    En France, les aliments concentrés totalisent 30 millions de tonnes de matières premières parmi lesquelles 64 % sont des céréales et 22 % des tourteaux d’oléagineux. Le déficit français en matières riches en protéines (dosant plus de 15 % de protéines) qui était d’environ 70 % dans les années 1980 a baissé en dessous de 40 % successivement grâce au développement du pois dans les années 1990 puis du colza depuis les années 2000 avec des surfaces totales cultivées de 2,5 millions d’hectares env...

  18. Processus ultra-rapides associés à la dynamique d'émission de la protéine GFP

    Science.gov (United States)

    Didier, P.; Guidoni, L.; Schwalbach, G.; Bigot, J.-Y.

    2002-06-01

    La protéine GFP (Green Fluorescent Protein) est un marqueur très efficace, utilisable en milieu vivant. La spectroscopie femtoseconde est particulièrement bien adaptée pour comprendre les mécanismes d'émission de cette protéine, étant donné la rapidité des processus de transfert mis en jeu. Nous-présentons des résultats sur la dynamique spectro-temporelle d'émission du mutant GFPuv résolue à l'échelle de la centaine de femtosecondes. Une transition Raman à 3300 cm^{-1} ainsi que la dynamique d'etablissement du gain avec un temps caractéristique d'environ 1.5 ps ont été mis en évidence.

  19. Améliorer l’évaluation de l’allergénicité de nouvelles protéines alimentaires : quels outils pour le futur ?

    OpenAIRE

    Denery-Papini, Sandra; Larré, Colette; Lollier, Virginie

    2016-01-01

    De nouveaux ingrédients protéiques vont être mis sur le marché et nécessiteront une évaluation du risque d’allergie alimentaire. L’article mentionne les limites des méthodes actuelles pour prédire ce risque et présente des méthodes in vitro et des modèles cellulaires en cours de développement choisis sur la base des étapes clés de l’allergie. Une approche pertinente doit prendre en compte à la fois la propriété des protéines à déclencher des réactions allergiques chez les personnes déjà aller...

  20. Desenvolvimento de um equipamento mecânico com controle numérico computadorizado para produção de protótipos em escala

    OpenAIRE

    2007-01-01

    A produção de equipamentos mecânicos corresponde a uma parcela importante da geração de bens de capital. Para a melhoria da produtividade e respostas mais rápidas às necessidades técnicas, lança-se mão de protótipos em escala que podem ser utilizados na avaliação do design, propriedades mecânicas, aspectos de fabricação, desempenho e funcionalidade na produção de peças e ou conjuntos. A produção de protótipos físicos pode ser feita através da utilização de equipamentos mecânicos com comando n...

  1. iDNA-Prot|dis: identifying DNA-binding proteins by incorporating amino acid distance-pairs and reduced alphabet profile into the general pseudo amino acid composition.

    Directory of Open Access Journals (Sweden)

    Bin Liu

    Full Text Available Playing crucial roles in various cellular processes, such as recognition of specific nucleotide sequences, regulation of transcription, and regulation of gene expression, DNA-binding proteins are essential ingredients for both eukaryotic and prokaryotic proteomes. With the avalanche of protein sequences generated in the postgenomic age, it is a critical challenge to develop automated methods for accurate and rapidly identifying DNA-binding proteins based on their sequence information alone. Here, a novel predictor, called "iDNA-Prot|dis", was established by incorporating the amino acid distance-pair coupling information and the amino acid reduced alphabet profile into the general pseudo amino acid composition (PseAAC vector. The former can capture the characteristics of DNA-binding proteins so as to enhance its prediction quality, while the latter can reduce the dimension of PseAAC vector so as to speed up its prediction process. It was observed by the rigorous jackknife and independent dataset tests that the new predictor outperformed the existing predictors for the same purpose. As a user-friendly web-server, iDNA-Prot|dis is accessible to the public at http://bioinformatics.hitsz.edu.cn/iDNA-Prot_dis/. Moreover, for the convenience of the vast majority of experimental scientists, a step-by-step protocol guide is provided on how to use the web-server to get their desired results without the need to follow the complicated mathematic equations that are presented in this paper just for the integrity of its developing process. It is anticipated that the iDNA-Prot|dis predictor may become a useful high throughput tool for large-scale analysis of DNA-binding proteins, or at the very least, play a complementary role to the existing predictors in this regard.

  2. Crescimento pulmonar compensatório (CPC: massa corpórea, conteúdo protéico e massa pulmonares em ratos subnutridos trilobectomizados

    Directory of Open Access Journals (Sweden)

    Ruiz Júnior Raul Lopes

    2004-01-01

    Full Text Available OBJETIVO:Avaliar o comportamento bioquímico do CPC após trilobectomia no rato adulto jovem subnutrido. MÉTODOS: Foram utilizados 137 ratos "Wistar", machos, subnutridos pela oferta de 33% da ingestão diária normal na fase de adaptação e durante o experimento, distribuídos por sorteio, em 9 grupos experimentais, submetidos a três tratamentos (Controle, Toracotomia, Trilobectomia e sacrificados em três momentos (7, 30 e 90 dias. Na Trilobectomia foram extirpados os lobos médio, acessório e caudal direitos, que representavam 55% do tecido pulmonar. Estudou-se os seguintes atributos e variáveis: massa corpórea e pulmonar, relação entre massa pulmonar e corpórea e conteúdos protéicos pulmonares. RESULTADOS: No lobo cranial e no pulmão esquerdo, tanto a massa quanto os conteúdos protéicos, nos trilobectomizados, foram maiores em todos os momentos do estudo quando comparados aos demais, sendo este aumento suficiente para compensar a perda dos três lobos. Os conteúdos protéicos do lobo cranial e do pulmão esquerdo, nos trilobectomizados, tiveram o mesmo comportamento da massa pulmonar, mas este aumento não foi suficiente para compensar a perda dos três lobos. CONCLUSÕES: Nos ratos adultos subnutridos trilobectomizados ocorre CPC. A recuperação da massa pulmonar é total, mas o conteúdo protéico pulmonar apesar de aumentar, não chega aos valores dos ratos não trilobectomizados.

  3. Desempenho operacional de um protótipo "aerossolo" Operational performance of an "aerossolo" prototype

    Directory of Open Access Journals (Sweden)

    Afonso Lopes

    2010-02-01

    Full Text Available O desempenho operacional de máquinas e equipamentos agrícolas é de fundamental importância para o conhecimento do comportamento desses em condições de trabalho. O presente estudo teve por objetivo avaliar o desempenho operacional de um protótipo "Aerossolo", tracionado por um trator Valtra BM100 4x2 TDA, com potência no motor de 73,6 kW (100 cv. O equipamento é de arrasto, constituído por comando hidráulico, cabeçalho com luva telescópica para regulagem longitudinal, duas seções dispostas lateralmente, contando com 28 lâminas em cada uma delas, lastros de concreto, rodas de transporte e rolo destorroador. O trabalho foi conduzido no Departamento de Engenharia Rural da UNESP -Jaboticabal, em delineamento em blocos casualizados, no esquema fatorial 3 x 3, com quatro repetições. A combinação dos nove tratamentos deu-se por três condições de lastragens (0; 960 e 1.307 kg e três ângulos das seções (0º; 7º e 14º. Os resultados evidenciaram aumento de 32,4% no consumo operacional (L há-1 relacionado à maior lastragem, bem como em 26,8% com o maior ângulo das seções, ambos comparados à condição sem lastro e com menor ângulo, respectivamente. A profundidade de trabalho, a força de tração e a potência na barra aumentaram com o aumento do ângulo das seções e com a adição de lastros.It is been a great deal of interest the studying of the operational performance of tractors and its machinery especially in work conditions. The present study had as objective to evaluate the operational performance of an "Aerossolo" prototype tracked with a Valtra BM100 4x2 TDA, with the engine power of 73.6 kW (100 cv. The prototype equipment is a drag type, hydraulically commanded, with telescopic heading for longitudinal adjustment, two lateral sections with 28 knifes in each one of them, concrete ballasts, wheels of transport and aggregate breaker coil. The work was conducted in the Department of Agricultural Engineering of

  4. 南通市区黄胸鼠和褐家鼠凝血酶原时间测定分析%Prothrombin time determination of commensal rodents in Nantong city

    Institute of Scientific and Technical Information of China (English)

    王智泉; 章士军

    2012-01-01

    目的 测定和分析南通市黄胸鼠和褐家鼠凝血酶原时间(PT).方法 PT测定采用仪器法,PT采用国际标准化比值(INR)表示.结果 黄胸鼠、褐家鼠及对照大白鼠的INR平均值分别为0.97、1.40和0.88;方差分析表明,黄胸鼠和褐家鼠之间的差异有统计学意义(t=0.426,P<0.05);褐家鼠和大白鼠之间的差异亦有统计学意义(t=-0.527,P<0.05);黄胸鼠和大白鼠之间差异无统计学意义(t=-0.101,P>0.05).崇川区与开发区的褐家鼠INR值经成组设计t检验差异有统计学意义(t=4.026,P<0.01),崇川区与港闸区的黄胸鼠经成组设计t检验差异无统计学意义(t=0.601,P>0.05).结论 实验所测得的黄胸鼠及褐家鼠INR值范围分别为0.60~1.92和0.84~2.29,PT值有性别、鼠种、区域间差异.%Objective To determine the prothrombin time (PT) of commensal rodents in Nantong city. Methods The instrument method was used for the determination of PT, which was expressed by international normalized ratio (INR). Results The average INR of Rattus tanezumi, R. Norvegicus and the laboratory rats was 0.97, 1.40 and 0.88 respectively. Statistical analysis showed that there was a significant difference in the INR between R. Tanezumi and R. Norvegicus (t = 0A26, P0.05) found. Also, a significant difference existed in the INR of R. Norvegicus from Chongchuan district and those from the development zone (? = 4.026, P0.05). Conclusion The INR of R. Tanezumi and R. Norvegicus is 0.60-1.92 and 0.84-2.29 respectively, with a difference in PT value between different genders, species and districts.

  5. Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T: a meta-analysis involving over 11,000 cases and 21,000 controls

    Science.gov (United States)

    Simone, B; De Stefano, V; Leoncini, E; Zacho, J; Martinelli, I; Emmerich, J; Rossi, E; Folsom, AR; Almawi, WY; Scarabin, PY; den Heijer, M; Cushman, M; Penco, S; Vaya, A; Angchaisuksiri, P; Okumus, G; Gemmati, D; Cima, S; Akar, N; Oguzulgen, KI; Ducros, V; Lichy, C; Fernandez-Miranda, C; Szczeklik, A; Nieto, JA; Torres, JD; Le Cam-Duchez, V; Ivanov, P; Cantu, C; Shmeleva, VM; Stegnar, M; Ogunyemi, D; Eid, SS; Nicolotti, N; De Feo, E; Ricciardi, W; Boccia, S

    2014-01-01

    BACKGROUND Genetic and environmental factors interact in determining the risk of venous thromboembolism (VTE). The risk associated with the polymorphic variants G1691A of factor V (Factor V Leiden,FVL), G20210A of prothrombin (PT20210A) and C677T of methylentetrahydrofolate reductase (C677T MTHFR) genes has been investigated in many studies. METHODS We performed a pooled analysis of case-control and cohort studies investigating in adults the association between each variant and VTE, published on Pubmed, Embase or Google through January 2010. Authors of eligible papers, were invited to provide all available individual data for the pooling. The Odds Ratio (OR) for first VTE associated with each variant, individually and combined with the others, were calculated with a random effect model, in heterozygotes and homozygotes (dominant model for FVL and PT20210A; recessive for C677T MTHFR). RESULTS We analysed 31 databases, including 11,239 cases and 21,521 controls. No significant association with VTE was found for homozygous C677T MTHFR (OR: 1.38; 95% confidence intervals [CI]: 0.98–1.93), whereas the risk was increased in carriers of either heterozygous FVL or PT20210 (OR=4.22; 95% CI: 3.35–5.32; and OR=2.79;95% CI: 2.25–3.46, respectively), in double hterozygotes (OR=3.42; 95%CI 1.64-7.13), and in homozygous FVL or PT20210A (OR=11.45; 95%CI: 6.79-19.29; and OR: 2.79; 95%CI: 2.25 – 3.46, respectively). The stratified analyses showed a stronger effect of FVL on individuals ≤45 years (p-value for interaction = 0.036) and of PT20210A in women using oral contraceptives (p-value for interaction = 0.045). CONCLUSIONS In this large pooled analysis, inclusive of large studies like MEGA, no effect was found for C677T MTHFR on VTE; FVL and PT20210A were confirmed to be moderate risk factors. Notably, double carriers of the two genetic variants produced an impact on VTE risk significantly increased but weaker than previously thought. PMID:23900608

  6. Risk of venous thromboembolism associated with single and combined effects of Factor V Leiden, Prothrombin 20210A and Methylenetethraydrofolate reductase C677T: a meta-analysis involving over 11,000 cases and 21,000 controls.

    Science.gov (United States)

    Simone, Benedetto; De Stefano, Valerio; Leoncini, Emanuele; Zacho, Jeppe; Martinelli, Ida; Emmerich, Joseph; Rossi, Elena; Folsom, Aaron R; Almawi, Wassim Y; Scarabin, Pierre Y; den Heijer, Martin; Cushman, Mary; Penco, Silvana; Vaya, Amparo; Angchaisuksiri, Pantep; Okumus, Gulfer; Gemmati, Donato; Cima, Simona; Akar, Nejat; Oguzulgen, Kivilcim I; Ducros, Véronique; Lichy, Christoph; Fernandez-Miranda, Consuelo; Szczeklik, Andrzej; Nieto, José A; Torres, Jose Domingo; Le Cam-Duchez, Véronique; Ivanov, Petar; Cantu-Brito, Carlos; Shmeleva, Veronika M; Stegnar, Mojka; Ogunyemi, Dotun; Eid, Suhair S; Nicolotti, Nicola; De Feo, Emma; Ricciardi, Walter; Boccia, Stefania

    2013-08-01

    Genetic and environmental factors interact in determining the risk of venous thromboembolism (VTE). The risk associated with the polymorphic variants G1691A of factor V (Factor V Leiden, FVL), G20210A of prothrombin (PT20210A) and C677T of methylentetrahydrofolate reductase (C677T MTHFR) genes has been investigated in many studies. We performed a pooled analysis of case-control and cohort studies investigating in adults the association between each variant and VTE, published on Pubmed, Embase or Google through January 2010. Authors of eligible papers, were invited to provide all available individual data for the pooling. The Odds Ratio (OR) for first VTE associated with each variant, individually and combined with the others, were calculated with a random effect model, in heterozygotes and homozygotes (dominant model for FVL and PT20210A; recessive for C677T MTHFR). We analysed 31 databases, including 11,239 cases and 21,521 controls. No significant association with VTE was found for homozygous C677T MTHFR (OR: 1.38; 95 % confidence intervals [CI]: 0.98-1.93), whereas the risk was increased in carriers of either heterozygous FVL or PT20210 (OR = 4.22; 95 % CI: 3.35-5.32; and OR = 2.79;95 % CI: 2.25-3.46, respectively), in double heterozygotes (OR = 3.42; 95 %CI 1.64-7.13), and in homozygous FVL or PT20210A (OR = 11.45; 95 %CI: 6.79-19.29; and OR: 6.74 (CI 95 % 2.19-20.72), respectively). The stratified analyses showed a stronger effect of FVL on individuals ≤ 45 years (p value for interaction = 0.036) and of PT20210A in women using oral contraceptives (p-value for interaction = 0.045). In this large pooled analysis, inclusive of large studies like MEGA, no effect was found for C677T MTHFR on VTE; FVL and PT20210A were confirmed to be moderate risk factors. Notably, double carriers of the two genetic variants produced an impact on VTE risk significantly increased but weaker than previously thought.

  7. MISS-Prot: web server for self/non-self discrimination of protein residue networks in parasites; theory and experiments in Fasciola peptides and Anisakis allergens.

    Science.gov (United States)

    González-Díaz, Humberto; Muíño, Laura; Anadón, Ana M; Romaris, Fernanda; Prado-Prado, Francisco J; Munteanu, Cristian R; Dorado, Julián; Sierra, Alejandro Pazos; Mezo, Mercedes; González-Warleta, Marta; Gárate, Teresa; Ubeira, Florencio M

    2011-06-01

    Infections caused by human parasites (HPs) affect the poorest 500 million people worldwide but chemotherapy has become expensive, toxic, and/or less effective due to drug resistance. On the other hand, many 3D structures in Protein Data Bank (PDB) remain without function annotation. We need theoretical models to quickly predict biologically relevant Parasite Self Proteins (PSP), which are expressed differentially in a given parasite and are dissimilar to proteins expressed in other parasites and have a high probability to become new vaccines (unique sequence) or drug targets (unique 3D structure). We present herein a model for PSPs in eight different HPs (Ascaris, Entamoeba, Fasciola, Giardia, Leishmania, Plasmodium, Trypanosoma, and Toxoplasma) with 90% accuracy for 15 341 training and validation cases. The model combines protein residue networks, Markov Chain Models (MCM) and Artificial Neural Networks (ANN). The input parameters are the spectral moments of the Markov transition matrix for electrostatic interactions associated with the protein residue complex network calculated with the MARCH-INSIDE software. We implemented this model in a new web-server called MISS-Prot (MARCH-INSIDE Scores for Self-Proteins). MISS-Prot was programmed using PHP/HTML/Python and MARCH-INSIDE routines and is freely available at: . This server is easy to use by non-experts in Bioinformatics who can carry out automatic online upload and prediction with 3D structures deposited at PDB (mode 1). We can also study outcomes of Peptide Mass Fingerprinting (PMFs) and MS/MS for query proteins with unknown 3D structures (mode 2). We illustrated the use of MISS-Prot in experimental and/or theoretical studies of peptides from Fasciola hepatica cathepsin proteases or present on 10 Anisakis simplex allergens (Ani s 1 to Ani s 10). In doing so, we combined electrophoresis (1DE), MALDI-TOF Mass Spectroscopy, and MASCOT to seek sequences, Molecular Mechanics + Molecular Dynamics (MM/MD) to

  8. The early diagnostic value of des-carboxy prothrombin in primary hepatocellular carcinoma%异常凝血酶原(DCP)对早期原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    赵婷婷

    2014-01-01

    Objective Explore diagnostic value of des‐carboxy prothrombin in primary hepatocellular carcino‐ma .Methods Alpha fetoprotein(AFP)and des‐carboxy prothrombin(DCP)levels in serum were detected by enzyme‐linked immunosorbent assay(ELLSA)and electro‐chemiluminescence .Among these samples 48 were with PHC ,35 were cirrhosis and 35 were healthy control group .The differences between the levels were compared and the value of DCP in diagnosis of PHC was assessed by ROC analysis .Results The value of DCP were(598 .3 ± 476 .2)ng/mL、(19 .2 ± 9 .8)ng/mL and(4 .8 ± 2 .5)ng/mL in the group of PHC ,cirrhosis and control group respectively ,and the AFP value were(710 .5 ± 409 .8)mAU/mL ,(34 .9 ± 8 .9)mAU/mL and(7 .9 ± 3 .1)ng/mL respectively .Both AFP and DCP levels in the group of PHC were higher than the other two group ,the difference between the two groups have statistical significance(P0 .05) ,while the specificity of DCP and AFP was 62 .2% and 71 .4% re‐spectively ,the difference has statistical significance(χ2 =4 .629 ,P<0 .05) .ROC curves indicated a better accuracy for DCP(AUC=0 .896)than AFP(AUC=0 .752)in diagnosis of PHC .Conclusion DCP is one of the good serologic in‐dicator in diagnose PHC and it could use in the clinical diagnosis .%目的:探讨异常凝血酶原(DCP)在早期原发性肝癌(PHC)中的诊断价值。方法分别采用酶联免疫法(ELISA)和电化学发光法对50例PHC患者、35例肝硬化患者、35例体检健康者进行治疗前血清 AFP和DCP测定,并比较各组水平差异,用受试者工作特征曲线(ROC曲线)比较评价DCP在 PHC早期诊断中的临床价值。结果 PHC组、肝硬化组和对照组AFP值分别为(598.3±476.2)ng/mL、(19.2±9.8)ng/mL和(4.8±2.5)ng/mL ;DCP的值分别为(710.5±409.8)mAU/mL、(34.9±8.9)mAU/mL和(7.9±3.1)ng/mL ;PHC组的 AFP和DCP均高于其他两组,差异有统计学意义(P<0

  9. Incorporação protéica de L[1-13C] leucina em ratos desnutridos:efeita da suplementação oral de glutamina.

    OpenAIRE

    Andrea Ferreira Schuwartz Tannus

    2004-01-01

    Não existe um consenso do efeito da suplementação de glutamina sobre a síntese protéica. No entanto, sugere-se que em animais desnutridos submetidos a trauma orgânico ou não, a glutamina além de seu papel no metabolismo energético, poderia ter efeito sobre a taxa de reciclagem protéica, tornando-se um aminoácido, condicionalmente, essencial. O objetivo deste trabalho foi determinar a incorporação protéica do aminoácido marcado 13C leucina em proteínas do plasma e da mucosa intestinal em ratos...

  10. Comportamento alimentar de ratos adultos submetidos à restrição protéica cujas mães sofreram desnutrição durante a lactação

    OpenAIRE

    PASSOS,Magna Cottini da Fonseca; Ramos,Cristiane da Fonte; TEIXEIRA Cíntia Vilanova; Moura,Egberto Gaspar de

    2001-01-01

    Foi avaliado o consumo alimentar de animais adultos submetidos à restrição protéica, cujas mães receberam dieta hipoprotéica ou hipocalórica na lactação: controle (C) ração normal com 23% de proteína; restrição protéica (RP) 8% de proteína; restrição energética (RE) 23% de proteína, em quantidade restrita à ingerida pelo grupo restrição protéica. Após o desmame todos os filhotes receberam ração normal até 60 dias e nesta época foram submetidos, por 21 dias, ao seguinte tratamento: (C/C)-filho...

  11. Cálculo das recomendações de ingestão protéica: aplicação a pré-escolar, escolar e adulto utilizando alimentos brasileiros

    OpenAIRE

    Marchini J. Sérgio; Rodrigues Margareth M.P.; Cunha Selma F.C.; Fausto M. Arlene; Vannucchi Helio; Oliveira J.E. Dutra de

    1994-01-01

    As recomendações de ingestão protéica variam conforme o teor de proteína, da quantidade de aminoácidos essenciais oferecidas e da digestibilidade da dieta. Em geral as recomendações protéicas tomam por base um alimento considerado padrão como o ovo ou o leite. No entanto, a nível populacional, ingere-se misturas de alimentos em quantidades e qualidades diferentes, que variam de região para região. A título de ilustração foram calculadas as recomendações de ingestão protéica para uma mistura d...

  12. Cálculo das recomendações de ingestão protéica: aplicação a pré-escolar, escolar e adulto utilizando alimentos brasileiros

    Directory of Open Access Journals (Sweden)

    Marchini J. Sérgio

    1994-01-01

    Full Text Available As recomendações de ingestão protéica variam conforme o teor de proteína, da quantidade de aminoácidos essenciais oferecidas e da digestibilidade da dieta. Em geral as recomendações protéicas tomam por base um alimento considerado padrão como o ovo ou o leite. No entanto, a nível populacional, ingere-se misturas de alimentos em quantidades e qualidades diferentes, que variam de região para região. A título de ilustração foram calculadas as recomendações de ingestão protéica para uma mistura de alimentos habitualmente usados pela população brasileira e consumida por três grupos etários diferentes: o pré escolar, o escolar e o adulto. São apresentadas 10 combinações de alimentos, todas tendo como principal fonte protéica o arroz e/ou o feijão. Observa-se que a recomendação de ingestão protéica do pré escolar, pode variar entre 1,15 a 1,77 g/kg por dia dependendo da mistura utilizada. Discute-se também a importância da quantidade de alimentos ingeridos e a presença de outros nutrientes. Sugere-se que ao se recomendar a ingestão protéica de uma determinada mistura de alimentos sejam considerados os seguintes fatores: teor total de nitrogênio, quantidade de aminoácidos essenciais, digestibilidade, peso total da mistura e a presença de outros nutrientes.

  13. Restauraciones protésicas sobre dientes con soporte periodontal reducido Prosthetic restoration on teeth with reduced periodontal tissue support

    Directory of Open Access Journals (Sweden)

    L.S. Osorio Vélez

    2009-10-01

    Full Text Available Cuando se involucra a los pacientes con enfermedad periodontal en un plan de tratamiento integral, se pretende, en primera instancia, controlar la enfermedad. Sin embargo, superado este objetivo, se espera y se requiere el reemplazo protésico de los dientes perdidos así como la rehabilitación de los remanentes en la mayoría de los casos. Considerando que la elección de los dientes pilares para un restauración protésica depende de la capacidad biológica del periodonto sano reducido de soportar a largo plazo la restauración, numerosas investigaciones se han diseñado para establecer los criterios determinantes en tal selección. Los resultados muestran que la principal limitación para el éxito de las restauraciones en pacientes con pocos pilares y una cantidad reducida de soporte periodontal, se relaciona con los factores biofísicos y técnicos y con el mantenimiento de la salud de los tejidos periodontales, más que con la cantidad de soporte periodontal.When it is intended to provide an integral treatment plan for patients with periodontal disease; it is aimed, at first, to control the illness. Nevertheless, once this objective has been overcome, it is expected and required, the prosthetic replacement of lost teeth as well as rehabilitation for remaining ones, in most of cases. Considering that the selection of the abutments for a prosthetic restoration depends on the biological capacity of their healthy reduced periodontal tissues supporting the restoration in the long term; a lot of research has been carried to establish the determining criteria for such selection. The results show that the limiting criteria for successful restorations in patients with few abutments and a reduced amount of periodontal support, is mainly related with biophysical and technical factors and with keeping healthy tissues, more than the periodontal support amount per se.

  14. Protocolo protésico - quirúrgico en temporización inmediata de dientes unitarios, utilizando una cubeta multifuncional desmontable Prosthetic protocol - surgery in the immediate temporization of a single tooth using a multifunctional removable tray

    OpenAIRE

    Y Leighton; JC Carvajal; Wolnitzky,A; R. SILVA; Von Marttens,A

    2010-01-01

    Propósito: El presente reporte clínico es la descripción de un protocolo protésico - quirúrgico que permite la visualización directa del sitio operatorio y el correcto posicionamiento protésico - implantario de una corona temporal atornillada, construida inmediatamente de insertado el implante. Material y métodos: Se seleccionaron 10 pacientes del programa de especialización en implantologia bucomaxilofacial de la Universidad de Chile, cuyo criterio de inclusión principal fue ser desdentado p...

  15. Función protésica inmediata con técnica All on-4 mandibular: Primer reporte al 1er año de carga inmediata

    OpenAIRE

    Y Leighton; Von Marttens,A; Carvajal,JC

    2014-01-01

    Propósito: El objetivo del presente estudio prospectivo es la observación del comportamiento de la función protésica inmediata sobre implantes axiales en combinación con implante inclinados posteriores distribuidos poligonalmente en mandíbulas edéntulas. Material y Métodos: De un grupo de 28 individuos desdentados completos mandibulares se observaron 25 individuos distribuidos en 13 hombres y 12 mujeres con un promedio de edad de 57.8 años, bajo la modalidad de rehabilitación protésica implan...

  16. Opciones no protésicas en el tratamiento de la artrosis de tobillo

    Directory of Open Access Journals (Sweden)

    DR. U. Giovanni Carcuro

    2014-09-01

    fomentar la realización de actividades deportivas sin o de bajo impacto. Respecto a los antiinflamatorios no esteroidales, no hay evidencia específica sobre la eficacia de estos en la literatura, pero ayudan de forma fiable a aliviar el dolor agudo asociado con la artrosis y en cierta medida con la inflamación. La suplementación con Glucosamina y Condroitín Sulfato tendría un efecto sobre la matriz extracelular y producción de ácido hialurónico, sin ser respaldado aún clínicamente en la literatura. Otra alternativa en el manejo es la infiltración con corticosteroides con un rol en tratamiento de los síntomas, pero no están exentos de riesgo. Su uso rutinario puede causar daños a los tejidos blandos alrededor de la articulación. Inyecciones de ácido hialurónico intra articulares se cree que ayudan por sus propiedades físicas y por su actividad antiinflamatoria, anabólica y efecto analgésico, con estudios que respaldan su uso. Alternativas como fisioterapia, centradas en medidas anti inflamatorias locales, fortalecimiento muscular, programas de reeducación de la marcha y ejercicios de movilización articular, modificación de calzado y uso de ortesis, pueden proporcionar alivio del dolor, mejora de la calidad de vida y posponer la cirugía en los pacientes afectados por artrosis de tobillo avanzada. Alternativas quirúrgicas no protésicas, como la artroplastia por distracción, preservar la superficie articular original y movimiento del tobillo. Los candidatos ideales son aquellos pacientes menores de 45 años que tienen artrosis postraumática moderada a severa del tobillo con una superficie articular relativamente congruente, dolor y movilidad articular bien conservada. La artroscopía permite la visualización directa, mínimamente invasiva y la manipulación de las estructuras intra articulares, ofreciendo una opción quirúrgica para el paciente adecuadamente seleccionado, mostrando 64–100% de buenos y excelentes resultados, pero con evidencia

  17. Four-factor prothrombin complex concentrate versus plasma for rapid vitamin K antagonist reversal in patients needing urgent surgical or invasive interventions: a phase 3b, open-label, non-inferiority, randomised trial.

    Science.gov (United States)

    Goldstein, Joshua N; Refaai, Majed A; Milling, Truman J; Lewis, Brandon; Goldberg-Alberts, Robert; Hug, Bruce A; Sarode, Ravi

    2015-05-23

    Rapid reversal of vitamin K antagonist (VKA)-induced anticoagulation is often necessary for patients needing urgent surgical or invasive procedures. The optimum means of VKA reversal has not been established in comparative clinical trials. We compared the efficacy and safety of four-factor prothrombin complex concentrate (4F-PCC) with that of plasma in VKA-treated patients needing urgent surgical or invasive procedures. In a multicentre, open-label, phase 3b randomised trial we enrolled patients aged 18 years or older needing rapid VKA reversal before an urgent surgical or invasive procedure. We randomly assigned patients in a 1:1 ratio to receive vitamin K concomitant with a single dose of either 4F-PCC (Beriplex/Kcentra/Confidex; CSL Behring, Marburg, Germany) or plasma, with dosing based on international normalised ratio (INR) and weight. The primary endpoint was effective haemostasis, and the co-primary endpoint was rapid INR reduction (≤1·3 at 0·5 h after infusion end). The analyses were intended to evaluate, in a hierarchical fashion, first non-inferiority (lower limit 95% CI greater than -10% for group difference) for both endpoints, then superiority (lower limit 95% CI >0%) if non-inferiority was achieved. Adverse events and serious adverse events were reported to days 10 and 45, respectively. This trial is registered at ClinicalTrials.gov, number NCT00803101. 181 patients were randomised (4F-PCC n=90; plasma n=91). The intention-to-treat efficacy population comprised 168 patients (4F-PCC, n=87; plasma, n=81). Effective haemostasis was achieved in 78 (90%) patients in the 4F-PCC group compared with 61 (75%) patients in the plasma group, demonstrating both non-inferiority and superiority of 4F-PCC over plasma (difference 14·3%, 95% CI 2·8-25·8). Rapid INR reduction was achieved in 48 (55%) patients in the 4F-PCC group compared with eight (10%) patients in the plasma group, demonstrating both non-inferiority and superiority of 4F-PCC over plasma

  18. 凝血酶原基因G20210A突变检测对肺血栓栓塞症的预测价值%Predictive value of prothrombin G20210A mutation detection in pulmonary thromboembolism

    Institute of Scientific and Technical Information of China (English)

    张佳; 赵凤芹; 谭平; 季红

    2014-01-01

    目的:探讨凝血酶原基因 G20210A(FⅡG20210A)突变在中国东北地区肺血栓栓塞症(PTE)患者中的发生频率,阐明检测该突变基因对中国东北地区人群 PTE的预测价值。方法:选择经核素肺灌注显像和(或)螺旋CT肺动脉造影(CTPA)结合临床症状确诊的PTE患者60例(病例组)和同期来自相同地区、性别和年龄相匹配的正常健康人80名(对照组)。应用蛋白酶消化及乙醇抽提获得2组研究对象的 DNA。采用聚合酶链式反应(PCR)、HindⅢ限制性内切酶片段多态性分析(RFLP )和琼脂糖凝胶电泳检测病例组和对照组研究对象FⅡG20210A基因突变情况。结果:经HindⅢ酶切后,病例组仅出现407和99 bp 2个条带,FⅡG20210A突变频率为0%,与对照组比较差异无统计学意义(P>0.05);病例组和对照组均未发现 FⅡG20210A基因杂合子或纯合子突变。结论:FⅡ G20210A基因突变在我国东北地区 PTE患者中发生率低,FⅡG20210A基因突变检测可能对中国东北地区人群 PTE无预测价值。%Objective To study the incidence frequency of prothrombin G20210A (FⅡ G20210A)mutation in the patients with pulmonary thromboembolism(PTE)in northeast China,and to clarify the predictive value of FⅡG20210A mutation detection in PTE of the population in northeast China.Methods 60 PTE patients(PTE group) and 80 sex-matched healthy controls(control group)from the same geographic area were selected.All the patients were diagnosed by lung ventilation/perfusion scan and/or multi-slice CT pulmonary angiography(CTPA)as well as medical history.The genome DNA was extracted from the whole blood using alcohol.Polymerase chain reaction (PCR),restriction fragment length polymorphisms(RFLP)analysis with HindⅢ restriction enzyme and sepharose gel electrophoresis were used to identify the F Ⅱ G20210A mutation in PTE group and control group. Results After digested by Hind

  19. Valores de referência do tempo de protrombina (TP e tempo de tromboplastina parcial ativada (TTPa em cães Reference ranges of prothrombin time (PT and activated partial thromboplastin time (aPTT in dogs

    Directory of Open Access Journals (Sweden)

    Sonia Terezinha dos Anjos Lopes

    2005-04-01

    Full Text Available Os fatores de coagulação são parte integrante da hemostasia normal, e tanto as coagulopatias hereditárias como adquiridas que envolvem este sistema são de grande importância veterinária, geralmente evidenciadas por manifestações clínicas, tais como, petéquias, equimoses, hematomas e sangramentos tardios. O presente trabalho teve por objetivo a determinação dos valores de referência do tempo de protrombina (TP e tempo de tromboplastina parcial ativada (TTPa, por métodos manuais. Para tanto, utilizou-se "kits" para dosagens humanas, devido à inexistência de produtos similares de uso específico veterinário. Foram utilizados 40 cães clinicamente sadios, sem raça definida, machos ou fêmeas, de diferentes idades. As amostras sanguíneas foram de 2,5ml cada, colhidas por venopunção cefálica e acondicionadas em tubos de centrífuga contendo 0,25ml de citrato de sódio a 3,8%. O plasma foi imediatamente separado por centrifugação e as determinações de TP e TTPa foram realizadas utilizando-se "kits" comerciais "HemoStat Thromboplastin-SIª" e "HemoStat aPTT-El b", respectivamente. Os resultados obtidos foram de 6,87 ± 1,4 segundos para o TP com valores mínimo e máximo de 4,07 e 9,67, respectivamente, e de 15,10 ± 1,6 segundos para TTPa com valores mínimo e máximo de 11,9 e 18,3, respectivamente. Conclui-se, que os valores obtidos neste trabalho podem ser utilizados como referência. Os reagentes utilizados para plasma humano podem ser empregados para o plasma de cães.The coagulation factors are part of normal hemostasis, and both hereditary and acquired coagulopathies that involve this system have an important role in veterinary medicine, generally evidenced by clinical signs such as: petechias, ecchymosis, hematomas and late hemorrhagies. The objective of this experiment was to determine reference range values of prothrombin time (PT and activated partial thromboplastin time (aPTT, for manual methods. Human commercial

  20. Um protótipo de gramática gerativa portuguesa: a gramática de Soares Barbosa

    Directory of Open Access Journals (Sweden)

    Edward Lopes

    2001-01-01

    Full Text Available

    Este trabalho estuda os aspectos doutrinários da Grammatica Philosophica da Lingua Portugueza, de Jerônimo Soares Barbosa, elaborada com base na doutrina da Grammaire générale et raisonnée de Port-Royal, para mostrar que ela é o protótipo iluminista de uma gramática gerativa da língua portuguesa. Essa gramática é a expressão das idéias iluministas no domínio da ciência da linguagem. Insere-se assim no embate ideológico que se travava, nessa época, entre as idéias absolutistas e a filosofia das Luzes. Essas posições manifestam-se, no nível dos estudos lingüísticos, pela gramática normativa, que estabelece um saber fazer, e pela gramática filosófica, que constrói um fazer.

  1. Revealing Both Sides of the Story: A Comparative Analysis of Mentors and Protégés Relational Perspectives.

    Science.gov (United States)

    Varga, Shannon M; Deutsch, Nancy L

    2016-10-01

    Mentoring continues to be a popular intervention for promoting positive youth development. However, the underlying mechanisms associated with sustainable and successful relationships remain largely unknown. Our study aimed to expand on previous literature by examining characteristics that have previously been linked to mentoring outcomes (e.g., authenticity, empathy), from a process-focused lens. We utilized post program satisfaction scores and interviews to examine the presence of each characteristic in a large sample of dyads (n = 144) as well as dyads' levels of agreement or disagreement about aspects of the relationships. We found that high satisfaction dyads demonstrated greater congruity and detail in their descriptions of their relationships, whereas low satisfaction dyads were highly divergent and inconsistent in their descriptions. In addition, misattunement, a negative relational aspect, was the most powerful distinguisher between high and low satisfaction dyads, which provides support for mentors receiving attunement training in order to reduce instances of misattunement. Findings from this study highlight the importance of examining and assessing mentoring relationships from both the mentor and protégé perspectives, as a single perspective may not present a full picture of the relationship.

  2. Streptococcus mutans in denture stomatitis patients under antifungal therapy = Streptococcus mutans em pacientes com estomatite protética submetidos à terapia antifúngica

    Directory of Open Access Journals (Sweden)

    Vasconcelos, Laurylene César de S.

    2010-01-01

    Full Text Available Objetivo: Verificar o número de Streptococcus mutans em saliva de pacientes com estomatite protética antes e após a terapia antifúngica. Metodologia: Após exame clínico de 93 pacientes, 47 foram selecionados para exame micológico e desta amostra foram selecionados trinta pacientes: 15 com diagnóstico positivo e 15 com diagnóstico negativo de candidose foram avaliados para contagem de S. mutans, determinação de fluxo salivar e capacidade tampão. Higiene bucal e da prótese, tempo de confecção, tipo de lesão e dados salivares foram relacionados com características clínicas e laboratoriais de Candida. Resultados: As lesões frequentes foram dos tipos I (43,5% e II (53,5%. A quantidade de S. mutans foi seis vezes maior em pacientes com candidose e foi associada com baixo fluxo salivar e higiene oral deficiente. Após a terapia, a redução de S. mutans foi verificada particularmente em pacientes com fluxo salivar normal. Os valores variaram de 0,01 a 3,88 UFC/ml x 104. Conclusão: Os dados sugerem que os Streptococcus colaboram com Candida spp na etiopatogenia da estomatite protética. O uso de agentes antimicrobianos orais pode propiciar efeito benéfico para pacientes com estomatite protética submetidos à terapia antifúngica e que apresentam higiene oral deficiente e parâmetros salivares desfavoráveis

  3. Representações ideativas sobre edentulismo e reabilitação protética na percepção de idosos

    Directory of Open Access Journals (Sweden)

    Silvana Orestes-Cardoso

    2015-09-01

    Full Text Available Objetivo: Identificar as representações ideativas de idosos edêntulos uni ou bimaxilares acerca das perdas dentárias e da reabilitação protética oral. Métodos: Estudo qualitativo, realizado entre janeiro e março de 2011 com sete idosos residentes em uma Instituição Pública de Longa Permanência do Recife-PE, com 14 idosos em atendimento na Clínica de Prótese Dentária da Universidade Federal de Pernambuco (UFPE. Coletaram-se os dados através de uma entrevista semiestruturada que passou por análise de conteúdo. Resultados: Os achados possibilitaram identificar que, para os idosos, os dentes contribuíam tanto para a saúde quanto como para facilitar interações sociais, enquanto o edentulismo foi associado a uma pluralidade de sentimentos negativos. Quanto à reabilitação protética, eles enfatizaram os prejuízos para a saúde devido a próteses mal adaptadas. Conclusão: Os idosos acreditam que o edentulismo e a reabilitação protética estão associados, principalmente, a um conceito mecanicista da profissão, amplamente difundido entre os profissionais que privilegiam mais a odontologia curativa em detrimento da prevenção. Nesse contexto, para que o envelhecimento possa ser considerado uma etapa da vida com as mesmas qualidades e dificuldades de qualquer outra, sugere-se aos gestores e aos próprios profissionais em saúde que se comprometam mais com uma prática odontológica humanizadora e preventiva, a fim de proverem os requisitos mínimos para um envelhecimento com dignidade.

  4. Platón y Aristóteles contra Protágoras : Estrategias de refutación del relativismo sofístico

    OpenAIRE

    Graciela Elena Marcos de Pinotti

    2002-01-01

    El propósito de este trabajo es individualizar y comparar los tratamientos que hacen Platón y Aristóteles de la doctrina de Protágoras. Ambos filósofos son fuentes importantes para su estudio, pero también los mayores críticos de la posición relativista tradicionalmente atribuida al sofista de Abdera. Los dos coinciden en que la tesis del hombre-medida, a la vez que introduce una forma de relativismo, se refuta a sí misma. Con todo, las líneas de ataque seguidas en cada caso son diferentes, p...

  5. Les sources de protéines dans l’alimentation du bétail en France : la place des oléoprotéagineux

    Directory of Open Access Journals (Sweden)

    Peyronnet Corinne

    2014-07-01

    Full Text Available En France, les aliments concentrés totalisent 30 millions de tonnes de matières premières parmi lesquelles 64 % sont des céréales et 22 % des tourteaux d’oléagineux. Le déficit français en matières riches en protéines (dosant plus de 15 % de protéines qui était d’environ 70 % dans les années 1980 a baissé en dessous de 40 % successivement grâce au développement du pois dans les années 1990 puis du colza depuis les années 2000 avec des surfaces totales cultivées de 2,5 millions d’hectares environ. Le tourteau de soja reste le premier tourteau utilisé en France mais ses importations baissent, notamment depuis 2009, et il n’a représenté que 3,3 millions de tonnes sur la campagne 2012/2013. L’utilisation du tourteau de soja non OGM diminue également avec 0,6 million de tonnes en 2012 contre 1,2 en 2002. Les tourteaux de colza et tournesol ont quant à eux atteint respectivement 2,3 et 1,4 millions de tonnes en 2012/2013 parmi lesquels 700 000 tonnes de tourteau de tournesol High pro (à 36 % de protéines importées de la zone Mer noire et 600 000 tonnes de différentes qualités produites en France. Depuis 2013, des tourteaux de tournesol High pro sont également produits en France à Bassens. Les prix des MRP suivent le marché mondial du tourteau de soja mais la demande française pour des matières premières non OGM permet une meilleure valorisation des sources de protéines locales.

  6. Protótipo de um sistema de gerenciamento de protocolos de câncer colorretal Management system prototype of colorectal cancer protocols

    Directory of Open Access Journals (Sweden)

    Huei Diana Lee

    2011-03-01

    Full Text Available OBJETIVO: desenvolvimento de um protótipo de sistema de cadastro e controle de protocolos de Câncer Colorretal, com a finalidade de armazenar um conjunto abrangente de dados de forma estruturada para posterior aplicação de métodos de análise inteligente de dados. MATERIAL E MÉTODO: o protótipo foi construído em cinco etapas: análise do domínio de Câncer Colorretal, definição de requisitos básicos (operacionais e informacionais, projeto do sistema, construção do sistema usando tecnologias open source e avaliação do sistema com o apoio de especialistas. RESULTADOS E DISCUSSÃO: durante o desenvolvimento, o protótipo foi avaliado por especialistas quanto à estrutura do conjunto de dados, à coerência de organização das informações, à validade das funcionalidades implementadas e ao atendimento aos requisitos básicos definidos. CONCLUSÃO: de acordo com os especialistas usuários, o desenvolvimento do protótipo completou a primeira etapa do projeto de desenvolvimento de um sistema em nível de 100% de satisfação e, atualmente, está sendo aplicado em cadastros de dados reais. Na próxima etapa será consolidado e implantado o sistema completo, atendendo a requisitos de segurança com suporte a multiusuáriosOBJECTIVE: to develop a prototype of a Colorectal Cancer records management system, aiming to store an embracing structured dataset for later application of intelligent data analysis methods. MATERIAL AND METHOD: the prototype was developed in five stages: Colorectal Cancer domain analysis, definition of basic requirements (operational and informational, system design, system construction using open source technologies and system evaluation supported by domain experts. RESULTS AND DISCUSSION: during development, the prototype dataset structure, coherence of information structure, validity of functions implemented and the attendance to the basic requirements define, were evaluated by domain experts. CONCLUSION

  7. Des sources de protéines locales pour l’alimentation des volailles : quelles voies de progrès ?

    Directory of Open Access Journals (Sweden)

    Bouvarel Isabelle

    2014-07-01

    Full Text Available L’aviculture européenne est dépendante d’approvisionnements en soja essentiellement en provenance du Brésil. Cette matière première riche en protéines est intéressante d’un point de vue nutritionnel pour l’alimentation des volailles mais pose des problèmes importants notamment en termes de prix et de conséquences environnementales. Les matières premières riches en protéines produites en France (oléo-protéagineux et coproduits de l’amidonnerie et de la distillerie ne remplacent qu’en partie le soja importé du fait d’équilibres nutritionnels moins propices. Les protéagineux sont de plus très peu disponibles sur le marché. L’aviculture européenne, et plus largement l’élevage et l’agriculture, sont ainsi face à des enjeux importants d’ordre économique, social et environnemental. Les contraintes et leviers relatifs à la formulation d’aliments destinés aux volailles sont analysés et différentes voies de progrès sont envisagées à plus ou moins long terme : développer l’alimentation de précision afin d’améliorer l’ajustement des apports aux besoins des animaux en fonction des objectifs fixés, disposer de matières premières adaptées (process, mis en œuvre de filières adaptées, nouvelles matières premières et additifs, sélection variétale mais aussi d’animaux adaptables. Une plus grande coordination entre acteurs, amont et aval, apparaît indispensable pour relever ces défis.

  8. Protótipos físicos e virtuais (CAD 3D: uma pesquisa descritiva sobre o processo de construção de uma saia godê

    Directory of Open Access Journals (Sweden)

    Gisely Andressa Pires

    2016-08-01

    Full Text Available Este artigo discute sobre a prática da construção de protótipos na indústria do vestuário. O protótipo refere-se à construção de um modelo que está em processo de planejamento e teste para ser comercializado. Atualmente a forma mais difundida é realizando a construção efetiva das peças, porém existe disponível no mercado softwares que realizam essa atividade virtualmente. Assim, esse estudo tem como objetivo investigar o processo construção de protótipos por meio de software CAD 3D de prototipagem de vestuário e das técnicas tradicionais manuais utilizadas atualmente na indústria a fim de verificar as caraterísticas de cada etapa e os resultados alcançados. Para tanto, foi desenvolvida uma pesquisa descritiva, como forma de abordagem do problema foram realizados dois experimentos que compreendem a construção de protótipos de uma saia godê orientados pela técnica virtual e a manual. Os resultados permitiram compreender que as duas possibilidades para a elaboração de protótipos produzem resultados similares, são eficientes e uma valiosa ferramenta para avaliar uma peça de vestuário antes de ser produzida a venda.

  9. A Meta-analysis of prothrombin G20210A polymorphism and its risk for sudden sensorineural hearing loss%凝血酶原基因G20210A多态性与突发性聋发病风险的Meta分析

    Institute of Scientific and Technical Information of China (English)

    刘博; 冯文静; 彭晓霞; 杨剑

    2013-01-01

    目的 采用Meta分析的方法探讨凝血酶原基因G20210A多态性与突发性聋发病风险的相关性.方法 计算机检索PUBMED、EMBASE、Cochrane图书馆(英文)和中国生物医学文献数据库(CBM),查找凝血酶原基因G20210A多态性与突发性聋发病相关的临床研究,对纳入的文献进行严格方法学质量评价,应用Review Manager Version 4.2软件进行统计分析.结果 共有9篇文献纳入研究,累计病例组735例,对照组1230例.方法学质量评价采用三方面8项计分(满分为8星),结果显示纳入的文献总体质量较高,其中2篇为8星,3篇为7星,1篇为6星,1篇为5星,2篇为4星.Meta分析统计结果提示凝血酶原基因G20210A多态性在基因型水平[OR=1.79,95% CI=(1.06,3.01),P=0.03]和等位基因水平[0R=1.77,95% CI=(1.06,2.97),P=0.03]均可能增加突发性聋的发病风险.结论 凝血酶原基因G20210A多态性可能增加突发性聋的发病风险,但仍需大样本的高质量研究进一步证实.%Objective To investigate the correlation between prothrombin G20210A polymorphism and the risk for idiopathic sudden sensorineural hearing loss (ISSNHL) using Meta-analysis methodology.Methods Databases,including PUBMED,EMBASE,Cochrane Library and CBM,were searched to collect the case control studies on the correlation between prothrombin G20210A polymorphism and idiopathic sudden sensorineural hearing loss.Only high quality studies were included.All analysis were conducted with Review Manager Version 4.2 software.Results A total of 9 studies were included,involving 735 cases and 1230 controls.The quality assessment involved 3 parts,8 scores (totally 8 stars).The results showed the included studies were high-quality.Two studies were 8 stars of quality,three studies were 7 stars,one study was 6 stars,one study was 5 stars,and two studies were 4 stars.Meta-analysis showed that the prothrombin G20210A mutation frequencies of the genotypes and alleles showed significant

  10. Reparación protésica de hernias inguinales con técnica de Lichtenstein

    Directory of Open Access Journals (Sweden)

    Ernesto Simón Enríquez Weinmann

    2003-03-01

    Full Text Available Se presentan los resultados de un estudio prospectivo que trata de la colocación de prótesis por vía anterior en la reparación de hernias inguinales con la utilización de variantes técnicas propuestas por Lichtenstein. Se incluye un total de 150 pacientes, a los que se les realizó operación, desde septiembre de 1996 hasta febrero de 2001; en 100 de éstos se practicó la técnica de reforzamiento protésico inicialmente descrita por Lichtenstein, y en 50, la técnica libre de tensión. Se realizaron 93 herniorrafias primarias, y 57 en hernias recidivadas, 10 de ellas iteradas. El tipo de hernia más frecuentemente operado según la clasificación de Gilbert modificada, fue la tipo III; 8 pacientes presentaban hernia en pantalón (tipo VI. La anestesia más frecuentemente usada fue la regional, y le siguió en frecuencia la anestesia general, local y analgesia acupuntural. Las complicaciones se comportaron de manera similar a las técnicas tradicionales, donde 26 pacientes presentaron complicaciones inmediatas menores. Ocurrió sepsis de la herida en un caso (0,6 %, y hasta la fecha no se han verificado recidivas, con un seguimiento promedio de 39 meses (12-66 del 100 % de los casos. Se sustenta la enseñanza del reforzamiento protésico para el cirujano en formaciónThe results of a prospective study dealing with the placement of prostheses by anterior route in the repair of inguinal hernias by using the technical variants proposed by Lichtenstein were presented. 150 patients who were operated on from September, 1996, to February, 2001, were included. The prosthetic reinforcement technique initially described by Lichtenstein was performed in 100, whereas the free-tension technique was used in 50 patients. 93 primary herniorraphies were performed. 57 were carried out in recurrent hernias, 10 of them iterated. Type III was the type of hernia most commonly operated on, according to Gilbert classification. 8 patients presented type VI hernia

  11. Características de carcaça de cordeiros terminados em confinamento, com dietas contendo diferentes níveis protéicos

    Directory of Open Access Journals (Sweden)

    Zundt Marilice

    2003-01-01

    Full Text Available Trinta e dois cordeiros (¹/2 Texel + ¼ Bergamácia + ¼ Corriedale, 16 machos inteiros e 16 fêmeas, foram alimentados durante 71 dias com dietas isoenergéticas (72% NDT, variando os níveis protéicos (12, 16, 20 e 24 % PB em baias individuais, com avaliação de carcaça após abate. O rendimento verdadeiro médio da carcaça foi 54%, enquanto o rendimento comercial médio foi 48%. Verificou-se que os níveis protéicos entre 12 e 24% não afetaram o peso da carcaça quente e fria; o rendimento comercial da carcaça, o índice de compacidade da carcaça e do pernil, bem como a condição corporal, conformação, cobertura de gordura, consistência da gordura, cor da carne, espessura de gordura e deposição de gordura. Os animais terminados com dietas com 12% de proteína bruta foram os que apresentaram maior rendimento verdadeiro de carcaça.

  12. Application of InterPro for the functional classification of the proteins of fish origin in SWISS-PROT and TrEMBL

    Indian Academy of Sciences (India)

    Margaret Biswas; Alex Kanapin; Rolf Apweiler

    2001-06-01

    InterPro (http://www.ebi.ac.uk/interpro/) is an integrated documentation resource for protein families, domains and sites, developed initially as a means of rationalizing the complementary efforts of the PROSITE, PRINTS, Pfam and ProDom database projects. It is a useful resource that aids the functional classification of proteins. Almost 90% of the actinopterygii protein sequences from SWISS-PROT and TrEMBL can be classified using InterPro. Over 30% of the actinopterygii protein sequences currently in SWISS-PROT and TrEMBL are of mitochondrial origin, the majority of which belong to the cytochrome b/b6 family. InterPro also gives insights into the domain composition of the classified proteins and has applications in the functional classification of newly determined sequences lacking biochemical characterization, and in comparative genome analysis. A comparison of the actinopterygii protein sequences against the sequences of other eukaryotes confirms the high representation of eukaryotic protein kinase in the organisms studied. The comparisons also show that, based on InterPro families, the trans-species evolution of MHC class I and II molecules in mammals and teleost fish can be recognized.

  13. Efeito do soro de leite no teor protéico e na qualidade tecnológica e sensorial de pães

    Directory of Open Access Journals (Sweden)

    Elessandra da Rosa Zavareze

    2008-08-01

    Full Text Available O trabalho teve como objetivo utilizar soro de leite in natura, concentrado e desidratado na formulação de pão e avaliar a influência deste ingrediente nas características tecnológicas e sensoriais do produto, assim como o aumento no teor protéico. Os resultados foram avaliados por análise de variância e as médias comparadas por teste de Tukey (p < 0,05. Os dados foram submetidos à correlação de Pearson. Os pães com soro de leite apresentaram maior teor protéico e notas tecnológicas superiores ao pão padrão, com exceção do pão com soro desidratado que apresentou menor volume específico. Na avaliação sensorial, os pães com adição de soro não apresentaram diferença significativa em relação ao pão padrão.

  14. Using ProtMAX to create high-mass-accuracy precursor alignments from label-free quantitative mass spectrometry data generated in shotgun proteomics experiments.

    Science.gov (United States)

    Egelhofer, Volker; Hoehenwarter, Wolfgang; Lyon, David; Weckwerth, Wolfram; Wienkoop, Stefanie

    2013-03-01

    Recently, new software tools have been developed for improved protein quantification using mass spectrometry (MS) data. However, there are still limitations especially in high-sample-throughput quantification methods, and most of these relate to extensive computational calculations. The mass accuracy precursor alignment (MAPA) strategy has been shown to be a robust method for relative protein quantification. Its major advantages are high resolution, sensitivity and sample throughput. Its accuracy is data dependent and thus best suited for precursor mass-to-charge precision of ∼1 p.p.m. This protocol describes how to use a software tool (ProtMAX) that allows for the automated alignment of precursors from up to several hundred MS runs within minutes without computational restrictions. It comprises features for 'ion intensity count' and 'target search' of a distinct set of peptides. This procedure also includes the recommended MS settings for complex quantitative MAPA analysis using ProtMAX (http://www.univie.ac.at/mosys/software.html).

  15. Návrh mechanismu pro zajištění pronace a supinace protézy horní končetiny

    OpenAIRE

    Rebenda, David

    2014-01-01

    Cílem této práce je návrh mechanismu pro zajištění pronace a supinace protézy horní končetiny a jeho následná realizace v podobě funkčního vzorku. Práce ve své teoretické části shrnuje základní typy amputací předloktí horní končetiny a jejich vliv na omezení rotace předloktí. Dále popisuje základní rozdělení protéz horní končetiny a typů pohonů. Konstrukční návrh včetě kompletní výkresové dokumentace je zpracován v programech Autodesk Inventor Professional 2013 a Rhinoceros 5.0 SR8. Prototyp ...

  16. Caracterización de la trombosis protésica en el servicio de cardiología de Las Tunas. 2006-2011

    Directory of Open Access Journals (Sweden)

    Maikel Santos Medina

    2015-11-01

    Full Text Available Se realizó un estudio descriptivo y transversal en el Servicio de Cardiología del Hospital “Dr. Ernesto Guevara de la Serna” de la provincia de Las Tunas, con el objetivo de caracterizar la trombosis de prótesis valvular en el período entre 2006 y 2011. El universo estuvo conformado por los 34 pacientes ingresados en el servicio por sospecha de trombosis protésica y la muestra por los 29 casos con diagnóstico definitivo de trombosis valvular protésica. Se utilizó la estadística descriptiva a través del análisis porcentual. La edad promedio de los casos fue de 47 años. Predominaron los pacientes del sexo femenino, con prótesis en posición mitral, con anticoagulación inadecuada y con ritmo sinusal en el electrocardiograma al ingreso. El edema agudo del pulmón fue la forma de presentación de disfunción ventricular izquierda que predominó. La mayoría de los pacientes fueron tratados con estreptoquinasa, lográndose resolución de la trombosis en la mayoría de los casos, con un número escaso de complicaciones tras el tratamiento trombolítico.

  17. 血清异常凝血酶原检测在原发性肝癌临床诊断中的应用%Application of serum abnormal prothrombin in clinical diagnosis of primary hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    濮珏彪; 王学锋; 彭奕冰

    2014-01-01

    Objective To investigate the significance of serum abnormal prothrombin [protein induced by vitamin K absence or antagonist-Ⅱ(PIVKA-Ⅱ)]in clinical diagnosis of primary hepatocellular carcinoma.Methods There were 365 samples from inpatients in Ruijin Hospital,including 100 patients with primary hepatocellular carcinoma and the other 265 patients with no primary hepatocellular carcinoma (59 cases of chronic liver disease,50 cases of gastrointestinal cancer,50 cases of secondary liver cancer,56 cases of other hepatopathy and 50 cases of healthy controls).Serum alpha fetoprotein (AFP)and PIVKA-Ⅱlevels were detected by Roche Cobas e601 automatic immunity analyzer and LUMIPULSE G1200 automatic immunity analyzer,respectively.Data were analyzed statistically by SPSS 16.0 software.Results Serum AFP and PIVKA-Ⅱlevels were significantly higher in primary hepatocellular carcinoma group than those in other disease group and healthy controls.Serum AFP had a sensitivity of 63.00%and a specificity of 84.91% in the diagnosis of primary hepatocellular carcinoma,while PIVKA-Ⅱhad a sensitivity of 74.00% and a specificity of 89.81%.The results of receiver operating characteristic (ROC)curve showed that the areas under the curve of AFP and PIVKA-Ⅱwere 0.789 and 0.873,respectively.The diagnosis sensitivity and specificity of the combination determination of AFP and PIVKA-Ⅱreached to 81.00% and 98.49%.Conclusions Serum PIVKA-Ⅱhas a better diagnosis significance than AFP,and can be a tumor marker in the diagnosis of primary hepatocellular carcinoma.Moreover,the combination determination of AFP and PIVKA-Ⅱcan improve the diagnosis efficiency for clinical primary hepatocellular carcinoma.%目的:研究血清异常凝血酶原[又称维生素K缺乏或拮抗剂Ⅱ诱导的蛋白质(PIVKA-Ⅱ)]检测在临床原发性肝癌诊断中的价值及意义。方法收集瑞金医院住院365例患者的血清,其中100例为原发性肝癌患者血清,265例为非原发

  18. A hermenêutica platônica no Íon e no Protágoras

    Directory of Open Access Journals (Sweden)

    José Renato de Araújo Sousa

    2003-01-01

    Full Text Available A forma mais simples e imediata de definir em poucas palavras a hermêutica é chamá-la de “arte ou técnica da interpretação”. Segundo Scheleimacher os gregos teriam sido os iniciadores dessa arte, quando buscavam interpretar os versos de seus poetas. Platão pode ser visto como um desses hermeneutas antigos das obras poéticas, ao lado dos sofistas que se proclamavam como os melhores intérpretes da arte de Homero. Neste artigo procuramos acompanhar um pouco dessa hermenêutica platônica apresentada por Sócrates nos diálogos Íon e Protágoras. As considerações da personagem Sócrates são importantes para entendermos a preocupação dos gregos em decifrar os enigmas da sua própria linguagem ao fazerem a exegese dos seus textos. To define hermeutics of the simple manner, direct, brief, we can call it “art or technique of interpretation”. According Scheleimacher the initiators of this practice were greeks, since they seeking to interpret the verse of his poets. Along with Sophists, Plato may be considered like one these antique hermeutical of poetic creation, that proclaimed themselves the best Homero’s hermeuticals. In this article we intend to understand something of this Plato’s hermeutics that Socrate dispute in the Ion and Protagora dialogues. The appreciations Socrate personage are very important to we understand in what manner the greeks were preoccupied to decode enigma of language them, making the exegesis the text own.

  19. Qualidade protéica da carne de novilho precoce alimentado com lipídios protegidos Protein quality of calves fed with protected lipids

    Directory of Open Access Journals (Sweden)

    Ivy Scorzi Cazelli Pires

    2006-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a qualidade protéica da carne de novilho precoce proveniente de quatro grupos genéticos de bovinos (GG alimentados com rações constituídas por lipídios protegidos ou não. Para a avaliação da qualidade protéica foram elaboradas oito dietas experimentais provenientes dos diferentes grupos genéticos e rações, as quais foram analisadas pelo PER (Coeficiente de Eficácia Protéica, NPR (Razão Protéica Líquida e digestibilidade in vivo. O experimento foi conduzido segundo um esquema fatorial (4 x 2 + 1 e as dietas experimentais e a padrão foram comparadas pelo teste de Dunnet, a 5% de probabilidade. Não foram encontradas diferenças significativas (P > 0,05 entre as dietas experimentais e a dieta padrão (caseína para PER, NPR e digestibilidade, sendo que os valores médios variaram entre 3,89 e 4,74 (98,06 e 119,34%, em relação à caseína; 4,80 e 5,65 (101,07 e 118,94%, em relação à caseína e de 92,49 a 95,48 (97,24 a 100,39%, em relação à caseína, respectivamente. Verificou-se que a carne de todos os grupos experimentais apresentou alta qualidade protéica, equiparando-se à da caseína, exceto o grupo genético Aberdeen Angus X Nelore, alimentado com a dieta protegida, que apresentou valor de NPR estatisticamente superior a esta.The objective of this study was to evaluate the protein quality of calves in four genetic groups (GG fed on a basal diet (D1 or a diet with protected lipids (D2. PER (Coefficient of Protein Efficacy, NPR (Liquid Protein Ratio and in vivo digestibility were analyzed for protein quality. The experiment was carried out according to a factorial scheme (four genetic groups X two diets in a completely randomized design. The PER, NPR and digestibility and values did not differ (P > 0.05 and the average values ranged from 3.89 to 4.74 (98.06 a 119.34% in relation to casein; 4.80 to 5.65 (101.07 to 118.94% in relation to casein and from 92.49 to 95.48 (97.24 a 100

  20. Avaliação da estomatite protética em portadores de próteses totais Evaluation of denture stomatitis in users of complete dentures

    Directory of Open Access Journals (Sweden)

    Terezinha Rezende CARVALHO de OLIVEIRA

    2000-09-01

    Full Text Available As próteses muco-suportadas são consideradas facilitadoras em potencial da estomatite protética (EP, lesão comumente observada sob a base das próteses, caracterizada por aspectos eritematosos difusos ou pontilhados na mucosa de suporte. Sua etiologia é controversa, podendo estar relacionada principalmente a fatores locais. Diversos aspectos funcionais associam-se a estes fatores representados pela oclusão, dimensão vertical, retenção, estabilidade dinâmica e estática além de aspectos qualitativos relacionados às condições encontradas no desdentado. Foram levados em consideração o período de edentulismo, número e tempo de uso das próteses, uso contínuo, conservação, presença de placa e higiene bucal. Os fatores foram avaliados, visando esclarecer a possível relação dos aspectos funcionais e protéticos com a EP. Exame clínico foi realizado em 116 pacientes de ambos os sexos, desdentados bimaxilares, que utilizavam as duas próteses muco-suportadas, portadores ou não de estomatite protética (EP. Os resultados indicaram que a maioria dos pacientes examinados tinha EP frente ao menor número de pacientes sem EP, havendo nos dois grupos equivalência nos resultados da avaliação clínica e protética. Os fatores funcionais e qualitativos das próteses, avaliados isoladamente, não puderam ser considerados responsáveis pela ocorrência dessa patologia, constatando-se que um único fator protético não demonstrou, de per si, ser responsável pela presença da EP, embora possa ser um facilitador para o seu desenvolvimento.Functional and qualitative factors in complete dentures can contribute to the development of lesions in the oral mucosa. Such lesions are classified as denture stomatitis (DS. There are considerable differences of opinion as to its etiology, mainly related to systemic and local factors. The influence of functional and qualitative features of complete dentures on denture stomatitis was evaluated. The

  1. Lifting DELLA Repression of Arabidopsis Seed Germination by Nonproteolytic Gibberellin Signaling1[C][W][OPEN

    Science.gov (United States)

    Ariizumi, Tohru; Hauvermale, Amber L.; Nelson, Sven K.; Hanada, Atsushi; Yamaguchi, Shinjiro; Steber, Camille M.

    2013-01-01

    DELLA repression of Arabidopsis (Arabidopsis thaliana) seed germination can be lifted either through DELLA proteolysis by the ubiquitin-proteasome pathway or through proteolysis-independent gibberellin (GA) hormone signaling. GA binding to the GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptors stimulates GID1-GA-DELLA complex formation, which in turn triggers DELLA protein ubiquitination and proteolysis via the SCFSLY1 E3 ubiquitin ligase and 26S proteasome. Although DELLA cannot be destroyed in the sleepy1-2 (sly1-2) F-box mutant, long dry after-ripening and GID1 overexpression can relieve the strong sly1-2 seed dormancy phenotype. It appears that sly1-2 seed dormancy results from abscisic acid (ABA) signaling downstream of DELLA, since dormant sly1-2 seeds accumulate high levels of ABA hormone and loss of ABA sensitivity rescues sly1-2 seed germination. DELLA positively regulates the expression of XERICO, an inducer of ABA biosynthesis. GID1b overexpression rescues sly1-2 germination through proteolysis-independent DELLA down-regulation associated with increased expression of GA-inducible genes and decreased ABA accumulation, apparently as a result of decreased XERICO messenger RNA levels. Higher levels of GID1 overexpression are associated with more efficient sly1 germination and increased GID1-GA-DELLA complex formation, suggesting that GID1 down-regulates DELLA through protein binding. After-ripening results in increased GA accumulation and GID1a-dependent GA signaling, suggesting that after-ripening triggers GA-stimulated GID1-GA-DELLA protein complex formation, which in turn blocks DELLA transcriptional activation of the XERICO inhibitor of seed germination. PMID:23818171

  2. Le processus de labellisation de l’Appellation d’Origine Protégée « Huile d’olive Bni Arouss »

    Directory of Open Access Journals (Sweden)

    Touriya Belaiche

    2015-10-01

    Full Text Available La reconnaissance des produits de terroir par une démarche de labellisation est l’un des axes d’intervention prioritaires du Plan Maroc Vert. Dans la région de Larache, il existe un savoir-faire ancestral en matière de greffage du rameau d’olivier (Olea Europaea sur des oléastres (Olea Sylvestris souvent centenaires. L’huile produite par ces oliviers greffés est prisée, à la fois pour son goût et pour les attributs qui lui sont prêtés, car ces arbres sont présents dans une zone ayant une importance culturelle et religieuse majeure. Nous avons mené un projet de recherche-intervention qui a visé à contribuer au maintien d'une oléiculture durable et rentable et à la préservation des savoir-faire traditionnels, par le biais de l'amélioration des pratiques de la filière et la labellisation. Cet article relate la méthodologie et les étapes que nous avons suivies pour accompagner les agriculteurs dans la démarche de labellisation et le dépôt d’une demande de reconnaissance d’Appellation d’Origine Protégée. Un premier diagnostic a permis d’identifier les problèmes de production, de récolte, de pressage et de conservation. Puis, l’aire géographique de l’appellation a été délimitée en partenariat avec un Groupement d’Intérêt Economique qui réunit les coopératives présentes dans la zone ainsi qu’avec les partenaires institutionnels. Enfin, un cahier des charges a été défini. Il décrit la future organisation de la production d’appellation protégée, au niveau des exploitations agricoles comme au niveau collectif. Le dossier d’Appellation d’Origine Protégée de l’« Huile d’olive Bni Arouss » a été déposé début 2014.

  3. Protótipo de sistema de documentação em enfermagem no puerpério Documentation system prototype for postpartum nursing

    Directory of Open Access Journals (Sweden)

    Regina Célia Sales Santos Veríssimo

    2013-01-01

    Full Text Available OBJETIVO: Desenvolver protótipo de sistema de documentação em enfermagem no puerpério. MÉTODOS: Planejamento de software utilizou modelo baseado em orientação a objetos, que englobou: compreensão e definição do contexto e dos modos de utilização projeto de arquitetura do sistema, identificação dos principais objetos do sistema, desenvolvimento dos modelos do projeto, especificação das interfaces dos objetos. Foram utilizadas as linguagem Structured Query Language (SQL, MySQL e Hypertext Preprocessor (php. RESULTADOS: O protótipo apresenta os requisitos planejados, entre eles: uso da Classificação Internacional para a Prática de Enfermagem (CIPE® versão 1.0 como código de apoio para execução do processo de enfermagem; apresentação dos eixos da CIPE® versão 1.0 em ordem de uso; geração de relatórios sobre a prática de uso dos processos de enfermagem. CONCLUSÃO: O protótipo de sistema de documentação proposto foi desenvolvido com sucesso com possibilidade de registros de enfermagem por meio de linguagem padronizada.OBJECTIVE: To develop a documentation system prototype for postpartum nursing. METHODS: For the software planning, a model based on object orientation was used, which included: understanding and definition of the context and usage modes of the system design project, identification of the main objects of the system, development of project models, specification of object interfaces. The languages Structured Query Language (SQL, MySQL and Hypertext Preprocessor (php were used. RESULTS: The prototype shows the planned requirements, among them: use of the International Classification For Nursing Practice (ICNP® version 1.0 as support code to perform the nursing process; presentation of the axes of ICNP® version 1.0 in order of use; elaboration of reports about the usage practice of nursing processes. CONCLUSION: The proposed documentation system prototype was successfully developed, allowing professional

  4. Microestructura y comportamiento plástico de perovsquitas conductoras protónicas de alta temperatura

    Directory of Open Access Journals (Sweden)

    López-Robledo, M. J.

    2005-10-01

    Full Text Available The creep behaviour of high temperature proton conducting perovskites SrCe0.9Y0.1O3-δ and Sr3Ca1.18Nb1.82O9-δ fabricated by laser fusion has been studied. Their microstructure has been studied both in the as-received and crept samples by means of Scanning Electron Microscopy (SEM and Electron BackScattering Diffraction (EBSD, to correlate plastic behaviour with the evolution of the microstructure. The materials show a cellular structure consisting of elongated grains embedded in an amorphous phase and a strong bi-axial crystallographic texture. Deformation tests at constant stress (creep tests have been performed both in Ar atmosphere and in air. Rigid grain rotation has been observed in the crept samples by EBSD. The amorphous phase flows outside the sample during creep. Plastic behaviour of these materials is independent of the environmental atmosphere and is consistent with a mechanism of viscous flow of the amorphous phase controlled by diffusion.

    Se ha estudiado el comportamiento en fluencia de perovsquitas conductoras protónicas de alta temperatura fabricadas por fusión láser, en particular los sistemas SrCe0.9Y0.1O3-δ y Sr3Ca1.18Nb1.82O9-δ. Se ha estudiado la microestructura antes y después de los ensayos mecánicos mediante Microscopía Electrónica de Barrido (MEB y Difracción de Electrones Retrodispersados (Electron BackScattering Diffraction, EBSD, con el objetivo de correlacionar el comportamiento plástico de estos materiales con su evolución microestructural. Los materiales analizados tienen una estructura celular de granos alargados, separados por una fase amorfa, y presentan una fuerte textura cristalográfica bi-axial. Se han realizado ensayos de deformación a carga constante (fluencia a diversas tensiones y temperaturas, en atmósfera inerte de Ar y en aire. Tras los ensayos, la fase intercelular fluye hacia el exterior de la muestra y se ha comprobado mediante EBSD que los granos rotan de forma rígida. El

  5. neXtA5: accelerating annotation of articles via automated approaches in neXtProt.

    Science.gov (United States)

    Mottin, Luc; Gobeill, Julien; Pasche, Emilie; Michel, Pierre-André; Cusin, Isabelle; Gaudet, Pascale; Ruch, Patrick

    2016-01-01

    implemented into the neXtProt annotation pipeline.Available on: http://babar.unige.ch:8082/neXtA5Database URL: http://babar.unige.ch:8082/neXtA5/fetcher.jsp.

  6. La zone humide de la Macta : un espace à protéger sur le littoral ouest de l'Algérie

    OpenAIRE

    Ghodbani, Tarik; Amokrane, Khélifa

    2013-01-01

    La Macta est un complexe de marais littoraux alimenté par un réseau hydrographique temporaire et dense. Cette zone humide abrite des biotopes et des biocénoses très riches. Elle représente de ce fait un pôle de biodiversité à l'échelle de l'Algérie et même du bassin méditerranéen. L'accentuation de la pression anthropique n'a pas cessé de modifier le fonctionnement et les caractéristiques de cet écosystème fragile. Le classement de la Macta, en 2001, comme zone humide à protéger dans le cadre...

  7. Desnutrição protéico-energética no paciente gastrectomizado Protein-energy deficiency in the gastrectomized patient

    Directory of Open Access Journals (Sweden)

    Silvia Justina PAPINI-BERTO

    2002-03-01

    Full Text Available Racional - A gastrectomia traz conseqüências nutricionais inevitáveis mas atenuáveis, dependendo da dietoterapia pós-operatória. Embora observada, essa desnutrição protéico-energética é pouco dimensionada, provavelmente, pela falta de consenso metodológico. Objetivo - Avaliar o grau de desnutrição protéico-energética do paciente gastrectomizado, utilizando-se de indicadores isolados ou combinados. Pacientes e Métodos - Foram estudados 71 pacientes com gastrectomia parcial (n = 53 ou total (n =18 em pós-operatório de 6 a 24 meses e 24-60 meses. Os dados dietéticos, composição corporal e bioquímicos foram analisados de acordo com o tipo de gastrectomia e tempo pós-operatório. Resultados - A cirurgia foi conseqüência de complicações de úlcera péptica (68% ou a câncer gástrico (32%. A perda de peso foi referida por 70% dos pacientes, sendo maior no grupo gastrectomia total (16 ± 5 kg do que no grupo gastrectomia parcial (10 ± 6 kg. Em geral, os pacientes apresentaram déficit antropométrico, albuminemia normal e baixa ingestão calórica, sugerindo deficiência energética crônica. A redução de hemoglobina, hematócrito e ferro ocorreu em maior intensidade e mais precocemente no grupo gastrectomia total. Assim, quando se associou hemoglobina aos indicadores albumina, linfócitos circunferência do braço e prega cutânea subescapular, a prevalência de desnutrição protéico-energética foi maior e em maior intensidade do que na ausência da hemoglobina. Conclusão - A gastrectomia resultou em desnutrição protéico-energética do tipo marasmática, acompanhada de anemia, mais intensa e precoce na gastrectomia total e gradativa na gastrectomia parcial, assemelhando-se à gastrectomia total no pós-operatório tardio.Background - Gastrectomy leads to nutritional consequences that although expected, are not usually measured due to methodological limitations. Aim - To assess the protein-energy deficiency degrees

  8. Influência de materiais alternativos nas propriedades de peças tubulares no contexto de moldes protótipo de injeção

    OpenAIRE

    Bareta, D. R.; Costa, C. A.; Zattera, A. J.; Pouzada, A. S.

    2008-01-01

    As técnicas de Ferramental Rápido surgiram como opção na obtenção de lotes protótipos de peças plásticas, onde o desafio é garantir que as peças obtidas nesses tipos de ferramentas possuam características o mais próximas das solicitadas em aplicações reais. O objetivo desse trabalho foi analisar e avaliar o uso de materiais alternativos como, resinas e ligas de alumínio e de zinco, em cavidades de moldes híbridos de injeção e suas influências nas peças finais. Nesse trabalho fo...

  9. Quid de l’utilisation des protéines d’origine végétale en aquaculture ?

    Directory of Open Access Journals (Sweden)

    Burel Christine

    2014-07-01

    Full Text Available Au cours des 30 dernières années, l’aquaculture mondiale n’a cessé de croître pour répondre à la demande croissante de poisson pour la consommation humaine qui ne peut plus être satisfaite par les seules captures de pêche. En conséquence, la demande de matières premières pour les aliments aquacoles augmente, tandis que le volume disponible de farine de poisson, ingrédient traditionnellement majoritaire dans ces aliments, reste stable. Cela a conduit à une substitution des ressources marines par des produits végétaux comme ingrédients pour les aliments pour poissons. La proportion de farine de poisson dans les aliments piscicoles a été réduite d’un tiers et est remplacée par une combinaison de sources protéiques végétales qui fournissent les acides aminés indispensables en quantité suffisante pour répondre aux besoins des poissons. Une large gamme de produits végétaux a été prospectée pour remplacer la farine de poisson. Sur la base de leur composition et de leur disponibilité sur le marché, les produits végétaux les plus utilisés en Europe sont des graines d’oléagineux (soja, colza, tournesol sous forme de tourteaux ou de concentrés protéiques, des protéagineux (lupin, féverole, pois, des céréales (maïs, blé ou des extraits de protéines obtenus à partir de céréales, comme par exemple les glutens. Néanmoins, les études ayant porté sur des taux de substitution très élevés (peu ou pas de farine de poisson ont mis en évidence des verrous physiologiques bien que les régimes contenaient tous les nutriments nécessaires : une baisse de la consommation alimentaire, de l’efficacité alimentaire et du taux de croissance des poissons, ainsi que des modifications métaboliques. Les travaux de recherche doivent être poursuivis afin d’améliorer l’efficacité nutritionnelle des produits végétaux via une réduction de leurs facteurs antinutritionnels et des toxines qu’ils contiennent

  10. Um Protótipo para Monitoramento em Tempo Real do Transporte Público de Porto Alegre - RS por GPS (Global Positioning System

    Directory of Open Access Journals (Sweden)

    Álan Cafruni Gularte

    2016-07-01

    Full Text Available Este trabalho apresenta o protótipo de uma solução de monitoramento de ônibus por GPS (Global Positioning System. O sistema é executado na plataforma Android, e dispõe de ferramentas que oferecem informações de posicionamento do veículo monitorado e estimativa de tempo de chegada do ônibus até a localização do usuário. Através deste sistema propõe-se uma solução que beneficie tanto as empresas que gerenciam as frotas de ônibus quanto os usuários do transporte coletivo, fazendo uso das facilidades que a mobilidade oferece.

  11. Análise da mutação G20210A no gene da protrombina (fator II em pacientes com suspeita de trombofilia no sul do Brasil Analysis of prothrombin G20210A mutation (factor II in patients with suspected trombophilia in Southern Brazil

    Directory of Open Access Journals (Sweden)

    Marcos Edgar Herkenhoff

    2012-04-01

    Full Text Available INTRODUÇÃO: A protrombina (fator II é uma proteína sanguínea sintetizada no fígado com a presença de vitamina K. É a precursora da trombina, que induz a formação de fibrina. Foi descrita uma mutação no gene da protrombina G20210A, associada diretamente a altos níveis de protrombina no sangue e, consequentemente, à trombofilia. Essa variante alélica consiste em mutação pontual, também chamada de polimorfismo de nucleotídeo simples (SNP, ocasionando a troca de uma guanina por uma adenina no nucleotídeo 20210, localizado em um sítio de clivagem do precursor do ácido ribonucleico mensageiro (mRNA. Essa troca caracteriza o alelo A e a ausência da mutação do alelo G. OBJETIVO: Quantificar o número de indivíduos homozigotos para alelo G, homozigotos para alelo A e heterozigotos, cujas amostras foram enviadas para o laboratório Genolab Análises Genéticas, abrangendo os estados do Paraná e Santa Catarina, no período de 1º de janeiro de 2009 a 10 de outubro de 2010. MÉTODOS: Análise de mutação pontual por reação em cadeia da polimerase em tempo real (RT-PCR. RESULTADOS: Obtivemos o número de 243 indivíduos e desse total 51,03% eram oriundos do estado do Paraná, enquanto 48,97%, oriundos do estado de Santa Catarina. Do total analisado, 88,89% possuíam o genótipo para homozigoto G, e nenhum indivíduo foi encontrado com mutação para homozigoto A. Apenas 11,11% possuíam genótipo heterozigoto. O estado de Santa Catarina apresentou frequência superior para genótipo heterozigoto em relação ao Paraná. CONCLUSÃO: Este estudo mostrou que é recomendável a identificação do genótipo para esse gene em pacientes com suspeita de trombofilia nos dois estados.INTRODUCTION: Prothrombin (factor II is a blood protein synthesized in the liver in the presence of vitamin K. It is a thrombin precursor, which induces fibrin formation. Prothrombin G20210A mutation and high prothrombin levels have been closely associated

  12. Descrição de dieta purificada para indução de quadro de desnutrição protéica em ratos

    Directory of Open Access Journals (Sweden)

    Márcia Queiroz Latorraca

    1998-02-01

    Full Text Available No presente trabalho é descrita a composição de dieta hipoprotéica (6% de proteína purificada para indução de quadro de desnutrição em roedores. A referida dieta foi padronizada em laboratório a partir de modificação da AIN-93 (documento do American Institute of Nutrition que estabelece os padrões nutricionais para roedores de laboratório, visando a obtenção de animais desnutridos para estudar as alterações metabólicas decorrentes da desnutrição protéica associada a situações como exercício físico, gestação e diabetes. A dieta em questão contém os seguintes componentes (g/ kg: amido de milho (480, caseína (71,5, dextrina de milho (159, sacarose (121, óleo de soja (70, microcelulose (50, mistura mineral AIN-93-G-MX (35, mistura de vitaminas AIN-93-G-VX, (10, L-cistina (1, cloridrato de colina (2,5. Ratos alimentados cronicamente com a dieta apresentaram sinais comumente presentes na desnutrição protéica humana e de animais de laboratório: redução do ganho de peso, hipoproteinemia, hipoalbuminemia, elevação dos ácidos graxos livres séricos e do glicogênio hepático.

  13. Estudo da biomassa de aguapé, para a produção do seu concentrado protéico

    Directory of Open Access Journals (Sweden)

    MEDEIROS Rosalina M. L.

    1999-01-01

    Full Text Available Na produção de concentrado protéico de aguapé (C. P. A., utilizou-se para extração da fração protéica solução de hidróxido de sódio (50mM na proporção (3:1 sendo três volumes de solução de hidróxido de sódio para um volume de planta desintegrada. A precipitação da proteína foi realizada utilizando solução de ácido clorídrico (100mM à temperatura de 80° C, o pH foi sendo corrigido até atingir o ponto isoelétrico da proteína, que ocorreu em pH 3,5. O teor de proteína encontrado no C. P. A. foi superior ao encontrado em outros vegetais e produtos de origem vegetal tais como alfafa e farinha de soja por exemplo. A presença de todos aminoácidos essenciais no C. P. A. mostra que sua utilização é viável na produção não só de ração animal, mas também, como suplementação alimentar na dieta humana.

  14. Efeito da desnutrição protéica precocemente corrigida na morfologia da epífise de crescimento de coelhos (Nova Zelândia)

    OpenAIRE

    2006-01-01

    Diversos autores comprovam a influência de um episódio de desnutrição crônica no crescimento pôndero-estatural. Entretanto, não são definidas as repercussões da desnutrição protéica precocemente corrigida sobre o potencial genético de crescimento, uma vez que é difícil distinguir o papel da desnutrição do ambiente onde habitualmente ocorre. Deste modo, esta pesquisa teve como objetivo analisar os efeitos da desnutrição protéica precocemente corrigida na morfologia da epífise de crescimento. A...

  15. Determinação da solubilidade protéica de farinhas de subproduto de aves com a pepsina em baixa concentração Determination of solubility of poultry by-product with pepsin in low concentration

    Directory of Open Access Journals (Sweden)

    Claudio Bellaver

    2004-10-01

    Full Text Available Foi proposto recentemente que a solubilidade protéica in vitro com pepsina na concentração de 0,0002% é melhor que em concentrações maiores, para melhor classificar a qualidade protéica de farinhas de origem animal (FOA. Entretanto, nessa concentração, desconhece-se qual é o intervalo que melhor exprime a qualidade por meio da solubilidade protéica das FOA. Este trabalho foi conduzido com o objetivo de identificar os valores da solubilidade protéica de farinhas de subprodutos do abate de aves (FSA, utilizadas como modelo experimental. As fontes protéicas foram duas FSA e caseína (considerada padrão com 100% de solubilidade protéica. Todas as farinhas foram autoclavadas a 120ºC, durante 0, 10, 20, 40 e 80 minutos. As concentrações utilizadas da pepsina em solução de HCl 0,0744N foram de 0,0002 e 0,02%. O delineamento experimental foi o de blocos casualizados em esquema fatorial 3 (fontes x 5 (temperaturas x 2 (concentrações, com quatro repetições para a enzima na concentração de 0,0002% e duas repetições para a concentração de 0,02%. Foram determinados os valores de solubilidade protéica in vitro em solução ácido/pepsina e energia metabolizável in vivo das FSA. Os resultados indicaram que, na concentração de pepsina de 0,0002%, os valores de boa qualidade das FSA estão acima de 55%. Da mesma forma, solubilidades próximas a 84% podem ser consideradas boas, mas decaem em solubilidade e são consideradas ruins se tiverem em torno de 67% de solubilidade protéica em pepsina a 0,02%.It was recently proposed that the in vitro protein solubility is better to classify animal by-product meals (ABP using pepsin concentration of .0002% than with higher concentrations. However on this concentration no reference value exists to express ABP quality based on protein solubility. The objective of this study was to obtain protein solubility values for poultry by-product meals (PBP, as model to ABP. Two PBP and casein

  16. Valor nutricional de farinha de trigo combinada com concentrado protéico de folha de mandioca Nutritional value of wheat flour mixed with cassava leaf protein concentrate

    Directory of Open Access Journals (Sweden)

    Ricardo Bryan Heinemann

    1998-06-01

    Full Text Available Alimentos não convencionais, como a folha de mandioca, contendo cerca de 20% de proteína em base seca, vêm sendo empregados freqüentemente na alimentação humana como alternativa alimentar. O uso da proteína da folha de mandioca na forma de concentrado possibilita eliminar parte do material residual da folha, facilitando sua incorporação a diversos alimentos habituais como cereais, aumentando seu valor protéico. Esta pesquisa visou avaliar a qualidade protéica de misturas feitas à base de farinha de trigo e concentrado protéico de folha de mandioca (CPFM, nos níveis de 5 e 10 % em relação ao peso da farinha. O CPFM foi obtido através da trituração das folhas frescas com hidróxido de sódio 0,1 N, seguido de filtragem, precipitação da proteína do "suco" através de fermentação natural, homogeneização do precipitado e desidratação em spray dryer. Determinou-se a composição centesimal do CPFM (proteína 36,36%, fibras 3,79%, lipídios 12,26%, cinzas 8,59% e carboidratos 39,0%. O CPFM apresentou um bom perfil de aminoácidos, exceto metionina que se apresentou como limitante. Apesar da farinha de trigo ter aumentado seu escore químico com a adição de CPFM, a deficiência de lisina ainda prevaleceu. Ensaio biológico foi conduzido para determinar a qualidade protéica das misturas de farinha de trigo com CPFM. Não houve diferença significativa no ganho de peso dos ratos alimentados com dietas de farinha de trigo com ou sem adição de CPFM, sendo todas inferiores à dieta de caseína. Os valores de eficiência líquida protéica foram respectivamente 2,03, 1,98, 2,15 e 4,61 para dietas com zero, 5 e 10% de CPFM e de caseína. Houve redução significativa da digestibilidade com adição crescente de CPFM à farinha de trigo, sendo de 99,6% para farinha sem adição, 96,6% para dieta com 5% e 90,1% para dieta com 10% de CPFM, em relação à caseína. Portanto, embora o perfil de aminoácidos e escore qu

  17. Quantificação de Polifenóis e Digestibilidade Protéica de Famílias de Feijoeiro Comum Polyphenol quantification and protein digestibility in common bean lineages

    Directory of Open Access Journals (Sweden)

    Carla Viviane C. Egg Mendonça

    2003-08-01

    Full Text Available O feijão é um dos alimentos encontrados em maior quantidade em todo o território nacional e é cultivado em quase todos os Estados. É uma importante fonte protéica na dieta do povo brasileiro, estando presente na alimentação da população rural e urbana. No presente trabalho, quantificaram-se os polifenóis e determinaram-se os níveis de digestibilidade protéica de cem famílias de feijoeiro comum (Phaseolus vulgaris L. obtidas do cruzamento entre os genótipos Amarelinho e CI 107. Os polifenóis afetam a qualidade do feijão, causando escurecimento, endurecimento e, ainda, podem reduzir a digestibilidade protéica. Pelos resultados obtidos, pôde-se encontrar 13 famílias com teores reduzidos de polifenóis e boa digestibilidade protéica in vitro, as quais poderão ser utilizadas em programas de melhoramento dessa importante cultura.Common beans are one of the most important food crops in Brazil and are cultivated in almost all Brazilian states. It is an important protein source for Brazilian people in both rural and urban areas. The present work performed the quantification of phenolic compounds and in vitro protein digestibility of one hundred common bean lines (Phaseolus vulgaris L. obtained by crossing the genotypes "Amarelinho" and CI 107. The results suggested that the phenolic compounds affected bean cooking traits and quality through darkening and hardening of the seed coat. Phenolic compounds also influenced the in vitro protein digestibility. It was observed that all 13 lines presented low levels of phenolic compounds and good in vitro protein digestibility and, as a result, must be incorporated in the breeding programs for this important crop.

  18. protVirt: simulação da dosagem de proteínas por espectrometria auxiliando as aulas práticas de Bioquímica

    OpenAIRE

    Gabriel Gerber Hornink

    2016-01-01

    The practical classes in the teaching of biochemistry could provide great contributions to the process of teaching and learning, and the understanding of these by students depend on, generally,  previous concepts about the experiment and procedures performed. It is presented in this paper the educational software protVirt, which could be used as a pedagogical innovation in the method of practical classes involving dosage of proteins, focusing on the development of skills for understanding the...

  19. 基于Netlogo平台的PROT项目融资管理仿真模型研究%Research on Simulation Model of PROT Project Financing Based on Netlogo

    Institute of Scientific and Technical Information of China (English)

    王艳伟; 黄宜

    2016-01-01

    PROT project financing is a complex and dynamic process.It is difficult to use the conventional meth-od to describe it vividly and clearly.The internal mechanism of PROT project cannot be explored.This paper at-tempts to use the netlogo simulation tool and means of system entropy to dynamically simulate,and to establish PROT project financing management simulation model based on netlogo.The simulation model can describe the change of the system entropy and the income status of the interests real-time dynamically,so as to promote the PROT project financing completed scientific and reasonable successfully.%PROT项目融资管理过程是一个复杂的、动态的多方博弈的过程,很难通过常规的研究方法全面而形象的将其清晰描述,也无法深入探究PROT项目管理的内在机理。本文尝试利用netl-ogo仿真工具,借助系统熵进行动态仿真模拟,建立基于netlogo的PROT项目融资管理仿真模型。通过该仿真模型能够实时动态的描述系统熵的变化以及各利益主体的收益状况,从而促进PROT项目科学合理的顺利完成。

  20. Concentrados protéicos para bovinos: 2. Digestão pós-ruminal da matéria seca e da proteína

    Directory of Open Access Journals (Sweden)

    Rodriguez N.M.

    2003-01-01

    Full Text Available O objetivo deste trabalho foi avaliar a digestão pós-ruminal de fontes protéicas. Utilizou-se a técnica de sacos de náilon móveis introduzidos no duodeno e colhidos nas fezes. Os alimentos introduzidos no intestino foram anteriormente incubados em sacos de náilon no rúmen ou foram separados das frações solúveis em água. O farelo de soja foi a fonte protéica de maior degradabilidade ruminal (73% e altíssima digestibilidade intestinal (98%. O glúten de milho foi a fonte protéica menos degradada no rúmen (16% e sua parcela sobrepassante foi altamente digerida no intestino (95%. Os farelos de algodão e de mamona mostraram características semelhantes com digestibilidade total da proteína de 95% e em torno de 65% de degradabilidade ruminal. O farelo de palmiste foi menos digerido (87% digestibilidade total quando incubado. Farinhas de origem animal foram menos digeridas no intestino e também no rúmen (fora o glúten, com digestibilidades totais de 75% para farinha de carne e ossos, 85% para farinha de penas e vísceras e farinha de peixe I, 67% para farinha de peixe II e apenas 46% para farinha de sangue.

  1. Retirada do tegumento e da extração dos pigmentos na qualidade protéica do feijão-preto Effect of removal of tegument and extraction of pigments on protein quality of black beans

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    Ana Cristina Nascimento Chiaradia

    1999-08-01

    Full Text Available O feijão é cultivado e consumido mundialmente e é a leguminosa mais consumida na América Latina. O baixo valor biológico das proteínas do feijão pode ser resultante, dentre outros, da presença de fatores antinutricionais presentes no tegumento do grão. Este trabalho visou avaliar, através de ensaios biológicos, o efeito da retirada do tegumento e das antocianinas e outros polifenóis do feijão-preto, na qualidade de suas proteínas. Foram analisados o quociente de eficiência protéica, o quociente de eficiência líquida protéica e a utilização líquida da proteína, e digestibilidade. Os resultados obtidos mostraram que a eliminação parcial do tegumento dos grãos cozidos e secagem em estufa diminuíram significativamente o valor do quociente de eficiência protéica comparativamente ao do grão integral cozido e secado nas mesmas condições. A extração, com etanol acidificado, de 27,5% dos polifenóis não influiu significativamente no valor protéico do feijão-preto. Concluiu-se que a retirada do tegumento e a extração parcial de polifenóis do feijão não elevaram a sua qualidade protéica.Bean seeds (Phaseolus vulgaris, L. are cultivated and consumed all over the world and are a staple food in most countries of South America. The low biological value of bean proteins may be due to, among others, the effect of antinutritional factors on the tegument. The objective of this work was to evaluate, through biological assays, the effect of removal of black bean tegument, anthocyanins and other polyphenols on protein quality. The protein efficiency ratio, the protein liquid efficiency ratio, the protein liquid utilization and the digestibility were analyzed. The results indicated that removal of the tegument of cooked beans reduced their protein value (PER when compared to whole cooked beans. The ethanolic extraction of 27.5% of polyphenols did not increase the protein quality of beans. It was concluded that removal of the

  2. Evaluation of orange peel and corn silages with different protein additivesAvaliações das silagens de bagaço de laranja e de milho com diferentes aditivos protéicos

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    Maria Socorro de Souza Carneiro

    2012-02-01

    Full Text Available Experimental mini-silos were prepared, in a completely randomized design distributed in a 2 x 5 factorial arrangement, with two silages (orange peel and corn and five treatments (without protein source; with urea; soybean meal; cotton seed meal and sunflower meal. The protein additives were added to each silage to achieve about 10% protein. The silos were opened 90 days after the make silages. The following determinations were accomplished: pH, lactic acid (LA, buffering capacity (BC, dry matter (DM, crude protein (CP, neutral detergent fiber (NDF, calcium (Ca, total carbohydrates (TC, non fibrous carbohydrates (NFC and in vitro digestibility (IVD. The protein additives increased the DM averages of the silages. The pH of silages varied from 3.41 to 3.95, being the lactic acid and in vitro digestibility higher to the orange peel silage when compared to the corn silage. It can be concluded that the orange peel silage to be preserved in silage form with protein source. Foram preparados minisilos experimentais, em delineamento experimental inteiramente casualizado, distribuídos em arranjo fatorial 2 x 5, sendo, 2 tipos de silagem (bagaço de laranja e milho e 5 tratamentos (sem fonte protéica; com uréia; farelo de soja; farelo de algodão e farelo de girassol. Os aditivos protéicos foram adicionados para que cada silagem obtivesse um teor protéico ao redor de 10%. Os silos foram abertos 90 dias após o preparo das silagens. Foram determinados pH, ácido lático (AL, capacidade tampão (CATP, matéria seca (MS, proteína bruta (PB, nitrogênio amoniacal (NNH3, extrato etéreo (EE, fibra em detergente neutro (FDN, cálcio (Ca, carboidratos totais (CT, carboidratos não fibrosos (CNF e digestibilidade in vitro (DIV. Os aditivos protéicos aumentaram os teores de MS das silagens. O pH das silagens variou de 3,41 a 3,95, sendo a produção de ácido lático e a digestibilidade in vitro maior para a silagem de bagaço de laranja quando comparada

  3. Efeito do níquel e da concentração protéica sobre o consumo e digestibilidade aparente da materia seca, proteína bruta, energia e balanço de nitrogênio do feno de Brachiaria brizantha cv. Marandu em ovinos

    OpenAIRE

    O.J. Paula; Graça,D.S.; Vasquez,E.A.; Martins,R.G.R.

    2005-01-01

    Avaliaram-se os efeitos do níquel e da concentração protéica da dieta sobre o consumo e a digestibilidade aparente da matéria seca (MS), proteína bruta (PB) e energia, e balanço de nitrogênio do feno de Brachiaria brizantha cv. Marandu, oferecido a ovinos. As dietas testadas foram: A- feno; B- feno + níquel; C- feno + mistura protéica; D- feno + níquel + mistura protéica. As dietas continham, respectivamente 0,2, 5,2, 0,5 e 5,5ppm de níquel e 3,1, 3,1, 8,2 e 8,2% de PB. Nos níveis com menor c...

  4. Desempenho de cordeiros alimentados com diferentes níveis protéicos Performance of lambs fed with different protein levels

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    Marilice Zundt

    2002-06-01

    Full Text Available O experimento foi conduzido com o objetivo de avaliar níveis crescentes de proteína bruta (PB na dieta de cordeiros em confinamento, por meio de seus efeitos sobre o desempenho animal. Trinta e dois cordeiros "tricross" (¹/2 Texel + ¼ Bergamácia + ¼ Corriedale, sendo 16 machos inteiros e 16 fêmeas, com idade média de 5 meses e peso vivo de 30 kg , foram alimentados durante 57 dias com dietas isoenergéticas (72% NDT, variando os níveis protéicos (12, 16, 20 e 24 % PB. Os animais foram identificados, pesados e distribuídos nos quatro tratamentos. Os cordeiros permaneceram em baias individuais, cobertas, com piso ripado e suspenso. Os parâmetros estudados foram ganho de peso, consumo de matéria seca, conversão alimentar e o custo de produção do quilograma de carcaça. A utilização de dietas, com diferentes níveis protéicos na terminação de cordeiros, mostrou efeito linear positivo para ganho de peso e negativo para conversão alimentar. Verificou-se que a utilização de dietas com níveis protéicos crescentes não influenciou o consumo de matéria seca dos cordeiros. Observou-se viabilidade econômica na terminação de cordeiros, utilizando-se dietas entre 12 e 24% de PB, entretanto, o maior retorno foi obtido com a ração contendo 12% PB. Não houve efeito de sexo para os parâmetros analisados.The experiment was carried out to evaluate increasing crude protein (CP levels in drylot lambs diet, and its effects on animal performance. Thirty two tricross lambs (1/2 Texel + ¼ Bergamácia + ¼ Corriedale, being 16 males and 16 females, with average age 5 months and live weight 30 kg, were fed for 57 days, with isoenergetic diets (72% TDN, but with different protein levels (12, 16, 20 and 24% PB. The animals were identified, weighted and distributed in the four treatments. The lambs were kept underroof in individual pens with lathing and a suspended floor. The studied parameters were weight daily gain, dry matter intake, feed

  5. Contribution of coagulases towards Staphylococcus aureus disease and protective immunity.

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    Alice G Cheng

    Full Text Available The bacterial pathogen Staphylococcus aureus seeds abscesses in host tissues to replicate at the center of these lesions, protected from host immune cells via a pseudocapsule. Using histochemical staining, we identified prothrombin and fibrin within abscesses and pseudocapsules. S. aureus secretes two clotting factors, coagulase (Coa and von Willebrand factor binding protein (vWbp. We report here that Coa and vWbp together are required for the formation of abscesses. Coa and vWbp promote the non-proteolytic activation of prothrombin and cleavage of fibrinogen, reactions that are inhibited with specific antibody against each of these molecules. Coa and vWbp specific antibodies confer protection against abscess formation and S. aureus lethal bacteremia, suggesting that coagulases function as protective antigens for a staphylococcal vaccine.

  6. Qualidade protéica do soro de leite fermentado pela levedura Kluyveromyces fragilis Protein quality of whey fermented by Kluyveromyces fragilis yeast

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    Caio Abércio da Silva

    2000-06-01

    Full Text Available O soro de leite fermentado pela levedura Kluyveromyces fragilis, após secagem em spray drier, foi submetido à avaliação da qualidade protéica através de uma análise aminoacídica e de um estudo biológico pelos métodos: Relação da Eficiência Protéica (PER, Relação da Eficiência Líquida da Proteína (NPR e Utilização Líquida da Proteína (NPU. Na análise aminoacídica, foram utilizadas como comparativo a composição de aminoácidos das proteínas padrão do ovo e da FAO. Os resultados indicaram um alto nível de lisina (65,60mg/g de proteína e baixos níveis de metionina e valina, respectivamente, 14,90mg e 45,80mg/g de proteína. A avaliação biológica foi conduzida durante 4 semanas, sendo utilizados 24 ratos desmamados aos 23 dias de idade. Os resultados para PER, NPR e NPU foram respectivamente, 19,75%, 46,33% e 26,54%, comparados com os valores obtidos com a proteína padrão caseína. A qualidade da proteína do soro seco fermentado apresentou-se inferior à proteína da caseína.Kluyveromyces fragilis fermented whey was spray dried and the protein quality of dried product was assayed by aminoacid analysis and three biological methods: Protein Efficiency Ratio (PER, Net Protein Ratio (NPR and Net Protein Utilization (NPU. Aminoacid analysis using casein and FAO protein as standards had a high level of lysine (65.6mg/g protein and low levels of methionine (14.9mg/g protein and valine (45.8mg/g protein. Biological evaluation of dried fermented whey protein, using 24 weaned rats, 23 days of age, during four weeks, using casein as standard protein, resulted in PER, NPR and NPU, 19.75%, 46.33% and 26.54%. Nutritional quality of the dried fermented whey protein was lower than the casein protein.

  7. Controle do tempo de protrombina em sangue capilar e venoso em pacientes com anticoagulação oral: correlação e concordância Comparative study of a portable system for prothrombin monitoring using capillary blood against venous blood measurements in patients using oral anticoagulants: correlation and concordance

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    Tiago Luiz Luz Leiria

    2007-07-01

    Full Text Available FUNDAMENTO: O uso de anticoagulantes orais (ACO é comum na prática cardiológica, tendo como principais indicações a fibrilação atrial e próteses valvares. Os pacientes em uso de ACO necessitam de controle freqüente do tempo de protrombina (TP. Novos sistemas portáteis de monitorização, para medida do tempo de protrombina, sem necessidade da coleta de sangue por venopunção, facilitam a rotina desses pacientes. OBJETIVO: Comparar as medidas do TP realizadas pelo sistema Coaguchek S®, em sangue capilar, com o método padrão em sangue venoso. MÉTODOS: Cento e vinte e sete pacientes em acompanhamento no ambulatório de anticoagulação do Instituto de Cardiologia foram submetidos ao método convencional e coleta de sangue capilar da polpa digital para medida com o sistema Coaguchek S®. RESULTADOS: A idade foi de 58±14 anos, 90% eram brancos. As indicações de ACO foram fibrilação atrial 49,6% e próteses valvares 37,0%. O coeficiente de correlação r s foi 0,90 (p 3,5 foi de Kappa 73,5%. O sistema Coaguchek S® superestimou o INR em 0,15±0,85 unidades. Valores acima de 3,5 unidades de INR mostram discrepância importante entre as duas técnicas. CONCLUSÕES: O sistema Coaguchek S® mostrou boa correlação e bom grau de concordância quando comparado com o controle, com resultados menores que 4. Porém, valores de INR acima de 3,5 ainda necessitam de confirmação com o método padrão.BACKGROUND: Oral anticoagulants (OAC are widely used in cardiology and are mainly indicated in cases of atrial fibrillation and prosthetic heart valves. Regular prothrombin time (PT control is required for patients using OAC. New portable monitoring systems for measuring prothrombin time, eliminate the need to collect blood by venous puncture and facilitate daily life for these patients. OBJECTIVE: To compare PT measurements using the Coaguchek S™ system with capillary blood and the standard method in venous blood. METHODS: One hundred and

  8. [Prevalence of thrombophilic mutations of FV Leiden, prothrombin G20210A and PAl-1 4G/5G and their combinations in a group of 1450 healthy middle-aged individuals in the Prague and Central Bohemian regions (results of FRET real-time PCR assay)].

    Science.gov (United States)

    Kvasnicka, Jan; Hájková, Jaroslava; Bobcíková, Petra; Kvasnicka, Tomás; Dusková, Daniela; Poletínová, Sárka; Kieferová, Veronika

    2012-01-01

    Factor V Leiden (G1691A) and prothrombin gene (FII G20210A) mutations are independent risk factors of venous thrombosis and this risk is further increased by the combined genotype 4G/4G PAI-1. The primary objective was to identify the frequency of mutations of minor alleles and genotypes of FVL, FII G20210A and PAI-1 4G/5G in healthy Caucasians in the Prague and Central Bohemia regions. The secondary objective was to identify the occurrence of their mutual combinations. Genotyping was performed in 1,450 healthy individuals (blood donors, 981 men and 469 women) using robotic DNA isolation and subsequent PCR and melting curve analysis (Light Cycler 480 System, Roche). The minor allele frequencies in FV Leiden and FII G20210A mutations were 4.5% and 1.3% respectively. The frequency of the 4G PAI-1 allele was 55.9%. The genotype frequencies were as follows: GG 91.10%, GA 8.83% and AA 0.07% for FV Leiden; GG 97.38%, GA 2.55% and AA 0.07% for FII G20210A and 4G/4G 30.69%, 4G/5G 50.34% and 5G/5G 18.97% for PAI-1. No differences in these frequencies were found between the genders. The occurrence of the combined heterozygous FII and heterozygous FV Leiden mutations was 0.14%. The PAI-1 4G/4G genotype was combined with the heterozygous FV leiden mutation in 2.83% of cases and with the heterozygous FII mutation in 0.62% of cases. The found frequencies of genotypes and alleles confirm a relatively high prevalence of hereditary thrombophilia in the Czech Republic.

  9. UNcleProt (Universal Nuclear Protein database of barley): The first nuclear protein database that distinguishes proteins from different phases of the cell cycle.

    Science.gov (United States)

    Blavet, Nicolas; Uřinovská, Jana; Jeřábková, Hana; Chamrád, Ivo; Vrána, Jan; Lenobel, René; Beinhauer, Jana; Šebela, Marek; Doležel, Jaroslav; Petrovská, Beáta

    2017-01-02

    Proteins are the most abundant component of the cell nucleus, where they perform a plethora of functions, including the assembly of long DNA molecules into condensed chromatin, DNA replication and repair, regulation of gene expression, synthesis of RNA molecules and their modification. Proteins are important components of nuclear bodies and are involved in the maintenance of the nuclear architecture, transport across the nuclear envelope and cell division. Given their importance, the current poor knowledge of plant nuclear proteins and their dynamics during the cell's life and division is striking. Several factors hamper the analysis of the plant nuclear proteome, but the most critical seems to be the contamination of nuclei by cytosolic material during their isolation. With the availability of an efficient protocol for the purification of plant nuclei, based on flow cytometric sorting, contamination by cytoplasmic remnants can be minimized. Moreover, flow cytometry allows the separation of nuclei in different stages of the cell cycle (G1, S, and G2). This strategy has led to the identification of large number of nuclear proteins from barley (Hordeum vulgare), thus triggering the creation of a dedicated database called UNcleProt, http://barley.gambrinus.ueb.cas.cz/ .

  10. Protéger la santé grâce à l’interdiction Prohibitions as Means of protecting Health

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    Claudine Viard

    2009-09-01

    Full Text Available L’édiction d’interdictions est un outil juridique que les autorités publiques utilisent couramment dans le but de protéger la santé. Des moyens importants en recherche doivent être mis en œuvre pour prouver la nocivité des produits ou d’activités qui ont été préalablement autorisés. Mais avant d’interdire, les obstacles à lever sont nombreux, car les produits et activités en cause ont une utilité, des sociétés importantes en vivent. C’est pourquoi, l’interdiction doit être aménagée, et les acteurs économiques être parfois indemnisés.Public authorities often resort to various forms of prohibition as means of protecting health. First, research has to be carried out in order to prove the harmful effects of previously authorised substances or activities. But before prohibiting these substances and activities, many obstacles have to be overcome. Indeed dangerous activities and products are usually useful in some respect, and large and successful companies make important profit out of them. This is why prohibition have to be worked out so as to allow a compensation of the loss suffered by economic actors.

  11. Modificaciones de diseño protésico para mejorar estética en prótesis parcial removible metálica

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    E. Fernández

    2013-08-01

    Full Text Available Pese al innegable incremento en la utilización de implantes óseo integrados en el tratamiento de la extensa problemática de la desdentación parcial, la confección de prótesis removibles metálicas continua siendo a escala mundial, el principal recurso terapéutico vigente. Sin embargo, desde el punto de vista del paciente, la recuperación de la función estética es su principal falencia. Proponemos entonces, extender la utilización del principio de retención friccional inherente a la prótesis fija, mediante la preparación de "facetas retentivas friccionales" en ciertos dientes pilares, generando de este modo artificios protésicos removibles con suficiente retención, sin exhibir elementos metálicos en los sectores anteriores de la cavidad bucal. Se exponen cinco casos clínicos que respaldan la validez nuestra propuesta tras una correcta indicación.

  12. Rehabilitación protésica con sobredentadura en una longeva Prosthetic rehabilitation with a overdenture in a long-lived woman

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    Marcia Hortensia Corona Carpio

    2010-12-01

    Full Text Available Se realizó un estudio donde se presenta un caso de una paciente longeva, femenina de 95 años de edad, de raza blanca, con buen estado de salud físico y mental, desdentada total superior y parcial inferior I clase de Kennedy, con experiencia protésica superior de 50 años y preocupación por rehabilitar su estética dental e interés por mantener sus dientes presentes. Se decidió realizar tratamiento con sobredentadura en los pilares presentes, se exponen técnicas y resultados obtenidos y con una evolución satisfactoria por parte de la paciente con el tratamiento alternativo realizado.A study was conducted to present the case of a long-lived white woman aged 95 with a good mental and physical state, edentulous total upper and partial lower with Kennedy's class I and experience in the use of upper prosthesis during 50 years and worried on its lower esthetics maintaining their teeths present. We decide to perform treatment with overdenture in present pillars. Techniques and results are showed, the patient evolving adequately with the alternative treatment carried out.

  13. Emulsifying and Foaming Properties of Different Protein Fractions Obtained from a Novel Lupin Variety AluProt-CGNA(®) (Lupinus luteus).

    Science.gov (United States)

    Burgos-Díaz, César; Piornos, José A; Wandersleben, Traudy; Ogura, Takahiro; Hernández, Xaviera; Rubilar, Mónica

    2016-07-01

    The use of vegetable proteins as food ingredient is becoming increasingly important due to their high versatility and environmental acceptability. This work describes a chemical characterization and techno-functional properties (emulsifying and foaming properties) of 3 protein fractions obtained from a protein-rich novel lupin variety, AluProt-CGNA(®) . This nongenetically modified variety have a great protein content in dehulled seeds (60.6 g protein/100 g, dry matter), which is higher than soybean and other lupin varieties. A simple procedure was utilized to obtain 3 different fractions by using alkali solubilization and isoelectric precipitation. Fractions 1 and 3 were mainly composed of protein and polysaccharides (NNE), whereas fraction 2 was mainly composed by protein (97%, w/w). Fraction 3 presented interesting and potential foaming properties in comparison to the other fractions evaluated in the study. Besides, its solubility, foaming and emulsifying capacity were practically not affected by pH variations. The 3 fractions also presented good emulsion stability, reaching values above a 95%. SDS-PAGE showed that fractions 1 and 2 contained mainly conglutin α, β, and δ, but in different ratios, whereas fraction 3 contained mainly conglutin γ and albumins. The results of this work will provide better understanding for the utilization of each protein fractions as potential ingredients in food industry.

  14. Immunogenicity and allergenicity of 2S, 7S and 11S soy protein fractions Imunogenicidade e alergenicidade das frações protéicas 2S, 7S and 11S da soja

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    Alvorita Leite Bittencourt

    2007-12-01

    Full Text Available It is known that in a part of the population, mainly among children, some are hypersensitive to soybean protein, although it is not yet completely elucidated which protein fraction is more immunogenic/allergenic. The objective of the study was to compare the immunogenicity and allergenicity of the soy protein fractions. The 2S (conglycinin, 7S (beta conglycinin and 11S (glycinin fractions were isolated from soy protein by affinity chromatography. These purified soy protein fractions were used as antigens for immunizing BALB/c mice to evaluate their immunogenicity by following the appearance of specific IgM and IgG antibodies in blood serum by ELISA. The allergenicity of these soy protein fractions was evaluated by the following approaches: i the production of IgE antibodies against 2S, 7S and 11S soy protein fractions by BALBc mice in the anaphylactic cutaneous passive test (PCA, and ii the production of IgG1 specific antibodies against the 7S fraction in BALB/c mice. The 7S and 11S fractions induced the formation of IgM and IgG antibodies. The PCA test showed that only the 7S fraction was allergenic leading to the production of IgE antibodies. Another evidence that reinforces the allergenicity of the 7S soy protein fraction is the presence of IgG1 specific antibodies reactive to this protein fraction in immunized mice. Our study shows that the 7S soy protein fraction is important to elicit allergic reactions in mice and may contribute to elucidate the allergenicity of soy-derived products.Sabe-se que uma parte da população, principalmente crianças, são hipersensíveis à proteína de soja, embora não esteja completamente esclarecido qual a fração protéica mais alergênica. O objetivo do estudo foi comparar a imunogenicidade e alergenicidade das frações protéicas 2S (conglicinina, 7S (beta conglicinina e 11S (glicinina da soja, isoladas por cromatografia de afinidade. Essas frações protéicas foram usadas como antígenos para

  15. Growth and feed utilization of the shrimp Farfantepenaeus paulensis fed diets containing different marine protein sources Crescimento e eficiência alimentar do camarão Farfantepenaeus paulensis alimentado com dietas contendo diferentes fontes protéicas marinhas

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    Ronaldo Olivera Cavalli

    2004-06-01

    Full Text Available Five isonitrogenous and isoenergetic diets containing different protein sources (fish, squid and mussel meals and their combinations at the proportions of 40, 30 and 30% or 60, 20 and 20% were fed for 28 days to Farfantepenaeus paulensis. Growth performance and feed utilization of shrimp fed the fish meal-based diet was comparatively inferior to those fed other protein sources or their combinations. This indicates that fish meal may not be the best protein source for F. paulensis. Feeds containing a mixture of protein sources are better utilized by F. paulensis and therefore result in significantly higher growth than those containing a single protein source.Cinco dietas iso-protéicas e iso-energéticas contendo diferentes fontes de proteína marinhas (farinhas de peixe, lula e mexilhão e suas misturas nas proporções de 40, 30 e 30% ou 60, 20 e 20% foram oferecidas durante 28 dias para o camarão Farfantepenaeus paulensis. O crescimento e a taxa de conversão alimentar dos camarões arraçoados com a dieta a base de farinha de peixe tendeu a ser inferior àqueles alimentados com as outras fontes de proteína e/ou suas combinações. Estes resultados indicam que a farinha de peixe pode não ser a melhor fonte protéica para F. paulensis. Dietas contendo uma mistura de fontes protéicas foram mais bem utilizadas por F. paulensis e, portanto, resultaram em taxas de crescimento superiores àquelas contendo uma única fonte de proteína.

  16. Protótipo de um sistema de aquecimento de água para população de baixa renda usando energia solar e elétrica, com reaproveitamento de calor

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    Marco Antônio Silva Pereira

    2016-01-01

    Full Text Available Este artigo consiste em uma pesquisa sobre os vários meios de aquecimento de água para o banho, buscando o uso de alternativas para viabilizar o acesso da população de baixa renda a esses benefícios. A ideia principal é fazer uma combinação dos vários sistemas existentes a fim de produzir um protótipo eficiente e de baixo custo. O protótipo constitui-se de um sistema de aquecimento solar, recuperador de calor e de um misturador de água automatizado. A representação do sistema foi obtida por meio de uma maquete do protótipo ecológico de chuveiro, englobando as diversas tecnologias estudadas.Palavras-chave: Aquecedor solar. Recuperador de calor. Misturador automatizado. Microcontrolador.Prototype of a water heating system for low-income population using solar energy and power, with reuse of heatAbstractThis paper consists of a survey on the various means of heating water for the bath, trying to use of alternatives to allow access of the low-income population. The main idea is to make a blend of various existing systems in order to produce an efficient and cost effective prototype. The prototype consists of a system of solar heating, heat recovery and an automated water mixer. The representation of the system was obtained through a model of the ecological prototype shower encompassing the several studied technologies.Keywords: Solar heating. Heat recovery. Automated mixer. Microcontroller.

  17. Ocurrencia de levaduras del género Candida y estomatitis protésica antes y después del tratamiento rehabilitador basado en prótesis removible

    OpenAIRE

    Lee Muñoz,Ximena; Cajas Cajas,Nataly; Gómez Carranza,Leyla; Vergara Núñez,Cristian; Ivankovic Silva,Mariana; Astorga Bustamante,Elizabeth

    2015-01-01

    El 63,2% de la población chilena mayor de 65 años utiliza prótesis removible. Cuando estas pierden funcionalidad pueden producirse lesiones en la mucosa oral, siendo la más prevalente la estomatitis protésica, proceso inflamatorio de la mucosa de soporte de diversa extensión y severidad, cuyo principal factor etiológico es la infección por Candida spp. El objetivo de esta investigación fue determinar la cantidad y las especies de levaduras del género Candida y su asociación con estomatitis pr...

  18. Terapia fibrinolítica en trombosis protésica de válvula biológica en posición aórtica

    OpenAIRE

    CARLOS A. CARLESSI; ATILIO M. ABUD; RICARDO FERNÁNDEZ

    2009-01-01

    RESUMENLa trombosis protésica es una complicación que se asocia con una mortalidad elevada. Lasque se presentan en bioprótesis son extremadamente infrecuentes, lo cual plantea un desafío terapéutico dado que su tratamiento, tanto en válvulas biológicas como mecánicas, escontroversial.Se describe el caso clínico de un paciente añoso con reemplazo valvular aórtico con implantede bioprótesis, que a los 6 meses presenta un cuadro de insuficiencia cardíaca aguda. En elecocardiograma transesofágico...

  19. Recobrimento de laranja 'Pêra' com filme de concentrado protéico de soro de leite associado a plastificantes 'Pêra' orange coating with whey protein concentrate film associated to plasticizers

    OpenAIRE

    Ana Cláudia Carraro Alleoni; Angelo Pedro Jacomino; Alexandra Soares Rosa

    2006-01-01

    O objetivo desse estudo foi avaliar o efeito de coberturas à base de concentrado protéico de soro de leite (CPSL), associadas a dois tipos de plastificantes (glicerol e sorbitol), em laranja 'Pêra'. As frutas foram lavadas e higienizadas de acordo com os padrões comerciais e submetidas à aplicação das coberturas. As coberturas à base de CPSL não foram eficientes na redução da perda de massa fresca da laranja 'Pera', independentemente do plastificante utilizado. Depois de 11 dias de armazenage...

  20. Avaliação microbiológica, física e mecânica de materiais resilientes modificados pela adição de antimicrobianos para tratamento de estomatite protética

    OpenAIRE

    2011-01-01

    No presente estudo, a proposta foi avaliar a ação antimicrobiana sobre o biofilme de Candida albicans (SC 5314) e determinar a mínima concentração inibitória (MCI) de cinco fármacos utilizados no tratamento de estomatite protética (nistatina, miconazol, cetoconazol, itraconazol e diacetato de clorexidina), quando incorporados em reembasadores resilientes temporários à base de resina acrílica (Trusoft e Softone) bem como o efeito dessa modificação sobre as propriedades de dureza Shore A e rugo...

  1. Desenvolvimento de protótipos de microaspersores através de prototipagem rápida e torneamento mecânico Development of microsprinkler prototypes through rapid prototyping and mechanical lathing

    Directory of Open Access Journals (Sweden)

    Alexsandro C. S. Almeida

    2010-07-01

    Full Text Available Objetivou-se, com este trabalho, construir protótipos de microaspersores, com boa uniformidade de distribuição para irrigações com sobreposição em hortas agrícolas, utilizando-se os processos de prototipagem rápida e torneamento mecânico. O estudo foi conduzido na ESALQ/USP e no Centro de Tecnologia da Informação Renato Archer (CTI. Utilizou-se o coeficiente de uniformidade de Christiansen na avaliação do desempenho dos emissores. O PVC e o tecnyl foram os materiais que apresentaram mais facilidade na construção dos protótipos no processo de torneamento mecânico, porém o PVC foi o escolhido devido ao melhor acabamento. Os protótipos construídos com torneamento mecânico apresentaram resultados satisfatórios ressaltando tratar-se de um processo bastante artesanal e laborioso; já com a prototipagem rápida se construíram protótipos com melhores acabamentos e mais rapidez. A utilização da prototipagem rápida no desenvolvimento de equipamentos de irrigação é algo totalmente inovador e de caráter pioneiro, além de uma ferramenta de grande utilidade na área de inovação de produtos no setor de Engenharia Agrícola.The present study had the objective of developing prototypes of microsprinkler with good distribution uniformity to irrigate with overlap in vegetable crops, by rapid prototyping and mechanic tournament. The work was carried out in the Hydraulic Laboratory of the Escola Superior de Agricultura "Luiz de Queiroz", University of Sao Paulo, Brazil, and in the Centro de Tecnologia da Informação Renato Archer (CTI. Two processes were tested in the development of the microsprinkler prototypes rapid prototype and mechanical lathing. Christiansen Uniformity Coefficient was used as indicator to evaluate the distribution uniformity. Among the tested materials, the PVC and the technyl showed more facility in the prototype development in the mechanical lathing process, however, the PVC was chosen due to better

  2. Projeto, construção e teste de protótipo de maca para transporte de crianças de um até dez anos de idades

    OpenAIRE

    Alan Ferreira Pinheiro Tavares

    2015-01-01

    Resumo: No transporte, por ambulâncias, as crianças estão expostas a riscos fatais ao serem transportadas em macas de adultos as quais não atendem a antropometria infantil. A situação é agravada ao considerar o fato das ambulâncias se locomoverem em altas velocidades e necessitarem frequentemente reduzi-las de modo abrupto. Neste cenário, buscou-se projetar, construir e testar um protótipo de maca com um sistema de retenção eficaz para transportes emergenciais de crianças. O diferencial do pr...

  3. Utilización del jugo de caña a voluntad con diferentes suplementos protéicos en pollos de engorde en finalización

    OpenAIRE

    Ramírez Náder Luis Miguel; Ramírez C. Carlos J.

    2010-01-01

    Se evaluó el efecto del suministro del jugo de caña a voluntad con diferentes suplementos protéicos en dietas para pollos de engorde sobre los rendimientos biológicos y los resultados económicos, comparados con una dieta balanceada convencional con base en el sorgo. Las variables experimentales consistieron en 4 dietas (tratamientos) que presentaron como fuente de variación el contenido proteico: tratamiento control (alimento balanceado con 18 % de proteína, sin jugo de caña), tratamientos T2...

  4. Influencia multifactorial en el desarrollo de contractura capsular, rotura protésica y complicaciones menores de las prótesis-expansoras en la reconstrucción de mama

    OpenAIRE

    2013-01-01

    Estudiamos de forma retrospectiva la evolución de 303 implantes mamarios tipo Becker en 231 pacientes reconstruídas en el Servicio de Cirugía Plástica del C.H.U.S. Se estudiaron posibles variables que pudieran influir en la presencia de complicaciones (contractura capsular, rotura protésica y complicaciones menores) y también en la supervivencia de estos implantes a largo plazo. De todas las variables de estudio, la radioterapia actuó como variable independiente aumentando el índice de com...

  5. Haematological alterations in protein malnutrition Alterações hematológicas na desnutrição protéica

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    Primavera Borelli

    2004-03-01

    Full Text Available Protein-calorie malnutrition (PCM is usually found in children, the elderly, patients suffering from neoplasia or chronic disease, patients undergoing chemotherapy, or even patients under parenteral nutrition. PCM presents a wide spectrum of signs and symptoms that are a result of not only the cause(s that led to malnutrition, but also of the different degrees of protein or carbohydrate deficiency. Here we present data obtained from observational and experimental epidemiological studies that suggest that malnourished individuals frequently present a greater susceptibility to infection with high morbidity and mortality indices. Data both found in literature and obtained by our group evidence that malnutrition modifies the organism's defence processes, impairing lympho-haematopoietic organs and modifying immune response. The haematological alterations in malnutrition, such as leucopoenia and hypoplasia, are described, with an emphasis on the results in experimental protein malnutrition obtained by our group. In particular, the structural and ultra-structural alterations of bone marrow, spleen and thymus; functional alterations such as the reduction of cell migration and spreading, phagocytosis, bactericidal and fungicidal activity as well as alterations in the production of reactive oxygen species are discussed. The implications of modifications of the haemopoietic environment in malnutrition states are still obscure, however, they seem to be responsible for inefficient haemopoiesis, especially inefficient myelopoiesis, and they seem to be irreversible over the short-term.Desnutrição protéico-calórica (DPC é geralmente encontrada em crianças, idosos, pacientes com neoplasias ou doenças crônicas, pacientes submetidos à quimioterapia ou à nutrição parenteral. A DPC apresenta uma variedade de sinais e sintomas que são um resultado não apenas da(s causa(s que provocam a desnutrição, mas também de diferentes graus de deficiência de

  6. The Dynamic Relationship Betweenthe Dyadic Work Engagement of both Mentors and Protégés and Job Performance%师徒的敬业程度与工作绩效之间的动态关系

    Institute of Scientific and Technical Information of China (English)

    陈为新; 汪纯孝; 刘芳; 温碧燕

    2015-01-01

    The authors have done an empirical study in four star Shanghai hotels to investigate the relationship between the dyadic work engagement of both mentorsand Protégés and job performance. The results of APIM analysis indicates that the actor’s and the partner’s engagement has signiifcant effects on the actor’s job performance, and the strength of the dyadic work engagement of both mentors and Protégés completely mediates the positive relationship between the partner’s engagement and the actor’s job performance.%作者在上海4家星级酒店进行了一次实证研究,探讨师徒的敬业程度与他们工作绩效之间的关系。行动者与伙伴相互关联模型分析结果表明:行动者和伙伴的敬业程度对行动者的工作绩效都有显著的影响,师徒双人组的敬业强度和高度与伙伴的敬业程度对行动者的工作绩效都有显著的交互效应。

  7. Avaliação in vivo da qualidade protéica do champignon do Brasil (Agaricusbrasiliensis Wasser et al. In vivo protein quality evaluation of champignon do Brasil (Agaricus brasiliensis Wasser et al.

    Directory of Open Access Journals (Sweden)

    Gilberto Simeone Henriques

    2008-10-01

    Full Text Available OBJETIVO: O trabalho aqui descrito trata da avaliação de uma dieta experimental contendo Champingnon do Brasil (Agaricus brasiliensis como fonte de proteína em um modelo experimental de ratos. MÉTODOS: Para este propósito, foram selecionados 24 ratos Wistar machos, recém desmamados (21 dias divididos em 3 grupos de 8 animais cada, que foram alimentados com uma dieta padrão de caseína, ou com uma dieta experimental de proteína de Agaricus brasiliensis ambas contendo 10% de proteína e isoenergéticas ou ainda, com uma dieta com muito baixo teor de proteína. O ensaio biológico foi realizado em 28 dias, ao longo dos quais se determinou a concentração de nitrogênio na urina e nas fezes, além dos cálculos do Quociente de Eficiência Alimentar (ganho de peso dividido pelo consumo de dieta, do Quociente de Eficiência Protéica (ganho de peso dividido pelo consumo de proteína, da Razão Protéica Líquida (ganho de peso corrigido dividido pelo consumo de proteína e da Digestibilidade Verdadeira. RESULTADOS: Os resultados demonstraram que quando o Champignon do Brasil foi utilizado como fonte exclusiva de proteína na dieta, os índices de qualidade protéica apresentaram-se baixos (Quociente de Eficiência Alimentar=0,08, Quociente de Eficiência Protéica=0,92 e Razão Protéica Líquida=3,00, quando comparados com a dieta padrão caseína (Quociente de Eficiência Alimentar=0,30, Quociente de Eficiência Protéica=3,05 e Razão Protéica Líquida=4,21. Os índices obtidos para o grupo Agaricus mostraram-se comparáveis àqueles apresentados por alguns tipos de proteína vegetal e podem ser explicados por sua limitação em aminoácidos essenciais, notadamente a lisina e a leucina, respectivamente primeiro e segundo aminoácido limitante. CONCLUSÃO: Os dados apontam para a utilização da proteína do Agaricus brasiliensis como uma boa fonte para complementação protéica, quando combinada com outras culturas vegetais comuns na

  8. Obstrucción de válvula protésica mitral tardía por trombo. Presentación de un caso clínico.

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    Gustavo Jesús Bermúdez Yera

    2011-10-01

    Full Text Available Las prótesis valvulares pueden presentar cuadros obstructivos a lo largo de su evolución, las causas más frecuentes son la trombosis y, a largo plazo, la instauración de tejido cicatrizal. La trombosis de una válvula protésica es una complicación que puede comprometer seriamente la vida. Se presenta el caso de un paciente portador de una obstrucción de prótesis valvular por trombo de aparición tardía, al cual se le realizó tratamiento quirúrgico con evolución satisfactoria. Al paciente se le había practicado una sustitución valvular por prótesis mecánica monodisco, 21 años atrás, y había presentado cuadros obstructivos en otros momentos, que se resolvieron con tratamiento médico. En esta ocasión fue necesario reintervenirlo quirúrgicamente, por la magnitud de la enfermedad, y sustituir, además de la válvula mitral protésica, la válvula aórtica nativa que también había enfermado.

  9. Tratamento clínico de endocardite em prótese valvar complicada por abscesso para-protético Successful medical management of prosthetic-valve endocarditis complicated by perivalvular abscess

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    Maria do Carmo Pereira Nunes

    2009-09-01

    Full Text Available O presente artigo relata o caso de um paciente do sexo masculino, 44 anos, com endocardite em prótese aórtica complicada por abscesso para-protético. Evoluiu com melhora do processo infeccioso apenas com o tratamento clínico. História prévia de doença reumática, submetido a três cirurgias cardíacas para troca valvar por disfunção de prótese e endocardite prévia. Neste relato de caso, discutiremos as características principais do abscesso para-protético como complicação de endocarditeWe present a case of a 44-year-old man with prosthetic aortic endocarditis complicated by a perivalvular abscess. He evolved with improvement of the infectious process only under clinical treatment. The patient presented a prior history of rheumatic fever and had previously been undergone three valve replacements due to prosthesis dysfunction and previous endocarditis. In this case report we discuss the main features of perivalvular abscess complicating infective endocarditis

  10. Validation of a new fiber electrode prototype for clinical electroretinography Validação de protótipo de um novo eletrodo de fibra para eletrorretinografia clínica

    Directory of Open Access Journals (Sweden)

    Adriana Berezovsky

    2008-06-01

    prototype provided stable and safe recordings of corneal electroretinograms compared to the commercially availabe DTL® electrode in healthy human subjects. The prototype is a feasible alternative instrument for clinical electroretinography recording to assess retinal function, however further analysis is recommended to validate its clinical usefulness in patients with retinal disorders.OBJETIVO: Validar um novo protótipo de um eletrodo de fibra para eletrorretinografia clínica (ERG. MÉTODOS: Foi testado em um grupo de 20 voluntários saudáveis (17-31 anos; média 22,7 ± 4,5; 8 homens, um protótipo de eletrodo de fibra com referência acoplado descartável recentemente desenvolvido (depósito de patente no Instituto Nacional de Propriedade Intelectual # PI0602186-7, constando de uma fibra de sinais corneanos e uma segunda fibra servindo como referência. Após 30 minutos de adaptação ao escuro, as respostas de cones e bastonetes da eletrorretinografia foram registradas simultaneamente com dilatação completa das pupilas em ambos os olhos, com o protótipo de eletrodo de fibra com referência acoplado em um olho escolhido ao acaso e um eletrodo DTL® no outro olho. Após a apresentação dos estímulos escotópicos e fotópicos cada resposta foi analisada em amplitude e tempo de culminação das ondas a e b. O sistema VERIS 5.1.9 foi usado para a aquisição e análise dos dados. Os resultados da eletrorretinografia foram analisados pelo teste de Mann-Whitney. Após a sessão da eletrorretinografia foi feito exame em lâmpada de fenda para avaliar possíveis eventos adversos. RESULTADOS: As respostas obtidas com o protótipo de eletrodo de fibra com referência acoplado foram comparáveis às do eletrodo DTL® comercialmente disponível. Numa análise qualitativa, o eletrodo de fibra com referência acoplado produziu sinais com menos ruído. Na média, a amplitude escotópica da eletrorretinografia e o tempo de culminação da onda-b usando o eletrodo DTL® foram

  11. Alimentação da piava (Leporinus obtusidens com diferentes fontes protéicas Piava's fed (Leporinus obtusidens with different protein sources

    Directory of Open Access Journals (Sweden)

    João Radünz Neto

    2006-10-01

    Full Text Available A piava (Leporinus obtusidens é um peixe nativo de grande importância nas bacias hidrográficas do Sul do Brasil. Neste estudo, verificou-se o crescimento de juvenis de piava alimentados durante 60 dias com três dietas contendo diferentes fontes protéicas: levedura de cana (L, farinha de carne e ossos (FCO e farelo de soja (FS. As biometrias foram realizadas a cada 20 dias para verificação de desempenho em peso, de comprimentos total e padrão, de taxa de crescimento específico, de fator de condição e de sobrevivência. Ao final do experimento foram calculados os rendimentos de carcaça, quociente intestinal e índices digestivo e hepato-somático. Verificou-se maior crescimento nos peixes alimentados com farelo de soja (FS, nos quais o peso e a taxa de crescimento específico foram superiores (PThe piava (Leporinus obtusidens is a important native fish in the South Brazil Rivers. In this study it was verified the growth of piava juveniles fed 60 days with three diets having different protein sources: yeast (L, meat and bone meal (FCO and soybean meal (FS. Fishes were measured each 20 days to obtain the performance in weight, total and standard length, specific growth rate, condition factor and survival. At the end of trial the carcass yield, intestinal quotient and the digestive and hepato-somatic index were estimated. Higher growth was verified in fish fed with soybean meal (FS, where the weight and specific growth rate were higher (P<0.05. Digestive index values were smaller in fish fed with meat and bone meal diets (FCO. The carcass yield was higher than 90%, had no significative differences. We concluded that soybean meal as main diet protein source provide good growth for piava juveniles (L. obtusidens.

  12. Corpos protéticos e corpos remodelados: elementos da cibercultura em anúncios publicitários de moda feminina na revista Elle

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    Adriana Tulio Baggio

    2014-12-01

    Full Text Available Sendo a cibercultura a cultura dominante da nossa sociedade pós-massiva, é razoável supor que seus elementos influenciem os produtos desta cultura, mesmo os não-massivos, como a publicidade de moda veiculada em revistas. Partindo desta premissa, este trabalho busca verificar a presença de aspectos da cibercultura em anúncios de marcas de vestuário feminino veiculados na revista Elle, especialmente as concepções de corpo protético e remodelado. A análise revela a presença destas concepções nos anúncios, ainda que algumas sejam mais sutis, devido à necessidade de aceitação dos anúncios e dos produtos pelas consumidoras. Considering cyberculture as dominant in our post-massive society, it is reasonable to assume that its elements influence the products of this culture, even the non-massive ones, like fashion ads in women's magazines. Starting from this premise, this paper seeks to verify the presence of aspects of cyberculture in women's clothing branding ads in Elle magazine, especially the concepts of prosthetic and remolded bodies. The analysis reveals the presence of these conceptions in ads, although some of them are more subtle, due to the need for acceptance of ads and products by consumers. The work is especially based on the cyberculture concepts of André Lemos (2008 and aspects of the post-human body of Lucia Santaella (2003 and Cristiane Mesquita (2004. 

  13. Procédé IFP de proction de protéines ex-paraffines Ifp Process for Protein Production Ex Praffins

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    Ballerini D.

    2006-11-01

    Full Text Available Cet article présente les grandes lignes du procédé ,IFP de production de protéines d'organismes unicellulaires, procédé réalisé avec un nouveau type de réacteur (jet-liquide Vogelbusch. Le microorganisme sélectionné est une souche de levure Candida tropicalis IFP 10, le substrat carboné employé étant une coupe de n-paraffines purifiées C18-C18. Avec le réacteur Vogelbusch, la fermentation est réalisée en un seul étage avec un taux d'hydrocarbures résiduels inférieur à 0,5 %o et une productivité élevée de l'ordre de 4 g " I-1 " h-1. Enfin sont résumés les résultats des tests toxicologiques et nutritionnels effectués sur la poudre de levures sèches. This article describes the leading aspects of the IFP process for producing singlecell proteins by means of a new type of reactor (Vogelbusch liquid jet. The microoganism chosen is a strain of Candida tropicalis IFP 10 yeast, while the carbon-containing substrate utilized is a C13-c18 cut of purified n-paraffins. With the Vogelbusch reactor, fermentation is done in a single stage with a residual hydrocarbon concentration lower than 0.5 %o and a high productivity of about 4 g-I-1- h r1. The results of toxicological and nutritional tests performed with dry yeast powder are summed up.

  14. Modulation de l'apoptose radioinduite par Ac-DEVD-CHO, un inhibiteur de protéases ``ice-like"

    Science.gov (United States)

    Weltin, D.; Holl, V.; Hyun, J. W.; Marchal, J.; Jung, G. M.; Dufour, P.; Bischoff, P.

    1998-04-01

    The “ICE-like" proteases, recently renamed caspases, are the human homologues of the Caenorhabditis elegans ced-3 gene product and are activated in the early steps of apoptosis. The aim of this work is to determine whether the inhibition of one of these proteases, namely caspase-3, is able to modify the cell sensitivity toward radiation-induced apoptosis. Murine spleen lymphocytes submitted to γ-radiations in presence of Ac-DVED-CHO, a caspase-3 specific inhibitor, exhibit a sharply reduced number of radiation-induced hypodiploid particules as compared to the controls and an almost total inhibition of the internucleosomal DNA fragmentation. However, both the anionic phospholipids externalisation, another specific hallmark of apoptosis, and the viability remain unchanged. Les protéases “ICE-like" ou caspases, sont les homologues humaines du produit du gène ced-3 du ver Caenorhabditis elegans et sont activées lors des étapes précoces de l'apoptose. L'objectif de ce travail vise à déterminer dans quelle mesure l'inhibition de l'une d'entre elles, la caspase-3 est susceptible de modifier la sensibilité des cellules vis-à-vis de l'apoptose radioinduite. Des lymphocytes spléniques murins irradiés en présence de Ac-DVED-CHO un inhibiteur spécifique de la caspase-3 présentent un taux de particules hypodiploïdes radioinduites bien inférieur à celui des contrôles et une diminution drastique de la fragmentation internucléosomale de l'ADN. Toutefois, ni l'externalisation des phospholipides anioniques, autre marqueur spécifique de l'apoptose, ni la viabilité ne sont affectées.

  15. Replacement of isoleucine-397 by threonine in the clotting proteinase factor IXa (Los Angeles and Long Beach variants) affects macromolecular catalysis but not L-tosylarginine methyl ester hydrolysis. Lack of correlation between the ox brain prothrombin time and the mutation site in the variant proteins.

    Science.gov (United States)

    Spitzer, S G; Warn-Cramer, B J; Kasper, C K; Bajaj, S P

    1990-01-01

    Previously, from the plasma of unrelated haemophilia-B patients, we isolated two non-functional Factor IX variants, namely Los Angeles (IXLA) and Long Beach (IXLB). Both variants could be cleaved to yield Factor IXa-like molecules, but were defective in catalysing the cleavage of Factor X (macromolecular substrate) and in binding to antithrombin III (macromolecular inhibitor). In the present study we have identified the mutation of IXLA by amplifying the exons (including flanking regions) as well as the 5' end of the gene by polymerase-chain-reaction (PCR) method and sequencing the amplified DNA by the dideoxy chain-termination method. Comparison of the normal IX and IXLA sequences revealed only one base substitution (T----C) in exon VIII of IXLA, with a predicted replacement of Ile-397 to Thr in the mature protein. This mutation is the same as found recently for IXLB. The observation that IXLB and IXLA have the same mutation is an unexpected finding, since, on the basis of their ox brain prothrombin time (PT, a test that measures the ability of the variant Factor IX molecules to inhibit the activation of Factor X by Factor VIIa-tissue factor complex), these variants have been classified into two different groups and were thought to be genetically different. Our observation thus suggests that the ox brain PT does not reflect the locus of mutation in the coding region of the variant molecules. However, our analysis suggests that the ox brain PT is related to Factor IX antigen concentration in the patient's plasma. Importantly, although the mutation in IXLA or IXLB protein is in the catalytic domain, purified IXaLA and IXaLB hydrolyse L-tosylarginine methyl ester at rates very similar to that of normal IXa. These data, in conjunction with our recent data on Factor IXBm Lake Elsinore (Ala-390----Val mutant), strengthen a conclusion that the peptide region containing residues 390-397 of normal Factor IXa plays an essential role in macromolecular substrate catalysis and

  16. Efeito de alguns curares naturais e da d-Tubocurarina retardando o tempo de coagulação e o tempo de protombina do sangue humano Natural curares and d-Tubocuratine effects on human blood clothing time and prothrombine time

    Directory of Open Access Journals (Sweden)

    João Canali

    1967-01-01

    Full Text Available In this paper the author points out to a old question of about 200 years ago in wich two kinds of opinions were discussed. BANCROFT and FONTANA in one hand atributes for the Indian arrow poison (curare the propriety of uncoagulate the blood, and C. BEBNAHDJ, B. RODRIGUES and others made an contradictory opinion upon this subject. In our experiments, we utilized 4 curares samples from indians who lives near the Brazilian border at Colombia, the famous Ticunas poison, and the alkaloid d-Tubocurarine. These poisons were added in form of emulsion in saline to the blood and blood plasma in order to perform two kinds of experiments. In one serie of experiments we observed the effect of curare on human blood coagulation time according to LEE-WHITE technic puting 0.5 ml of the various poisons emulsions previously into the tube. By this method, we have found that the emulsion containing 0.1 g of the poison in 10 ml saline was the most effective (Table II, therefore we used this curare emulsion concentration in the other serie of experiments, in which we tested the action of these venoms on the human blood plasma prothrombins time, (Quick Technic adding 0.1 ml of the saline poison emulsion to each 0.1 ml of human blood plasma. Results from these experiments can be seen on Table II. These experiments we have tried on one sample of human blood plasma plus the differents curares samples; and in another opportunity four samples of human blood plasma were tried with the curare from Ticunas indians (the most effective in this respect. Results from these experiments may be seen on Table III. All the poison tried in our experiments was previously tested on toads legs (B. crucifer to verify his curares action. All times obtained with the experiments above, show highly significant results (P<001 when compared with the blood and blood plasma mixed with in the same volume of saline. Our results, point out that BANCROFT and FONTANA views upon the effect of curare on

  17. Protein concentrate obtainment from leaves and aerial part cassava (ManihotesculentaCrantzObtenção de concentrado protéico de folhas e parte aérea da mandioca (ManihotesculentaCrantz

    Directory of Open Access Journals (Sweden)

    Janaina Lima da Silva

    2012-12-01

    Full Text Available The aerial parts of cassava, constituted by leaves, stalk sand stems, are considered as agro-industrial waste, so, they are thrown away during roots crop. This material has content of protein, vitamin and mineral; therefore, it can be used as a dietary supplement for food industries. Thus, alternatives have come to extract protein from leaves and remove toxic agent sand anti-nutrients that make part of their composition. In this context, this study aimed at evaluating yield, mineral composition and functional properties of protein concentrates from leaves and aerial part of cassava. During the extraction of proteins, the following tested methods were:(1 isoelectric precipitation; (2 natural fermentation for five days; (3 fermentation for 48 hours and (4 fermentation for 48 hours, followed by pH adjustment. A 2 x 4 factorial design was used, the studied factors were the products (leaves and shoots and the method of protein extraction (four methods, with three replications. From the results, it was observed that Method1 provided the highest yields of protein concentrate and protein extraction for cassava leaves, however, there was no significant difference among the extraction methods for the aerial part of cassava. The values of Fe, Mn and Zn increased in protein concentrates obtained both in leaves and the aerial part of cassava, especially for Method 3. The capacities of absorbing water and oil from protein concentrates were considered high for the four studied methods, thus, indicating a good application in food products.As partes aéreas da mandioca, representadas pelas folhas, hastes e caules, constituem-se como resíduos agroindustriais por serem desperdiçadas na colheita das raízes. Esse material possui valor protéico, vitaminas e mineiras, propiciando sua utilização como suplemento alimentar nas indústrias alimentícias. Alternativas neste sentido surgem para extrair a proteína das folhas e eliminar os agentes tóxicos e

  18. PROTEINES Adaptation des stratégies d’approvisionnement des filières animales en matières premières riches en protéines

    Directory of Open Access Journals (Sweden)

    Lapierre Olivier

    2002-03-01

    Full Text Available L’embargo américain sur le soja en 1973, l’interdiction plus récente de l’utilisation des produits d’origine animale ou la position de la Commission européenne sur l’opportunité d’un « Plan protéine » sont autant d’éléments qui posent la question de l’autonomie protéique des cheptels de l’Union européenne. Le modèle « Prospective aliment », développé par le Cereopa afin d’analyser les stratégies d’approvisionnement en matières premières des fabricants d’aliments, révèle une augmentation de l’ordre de 40% de l’utilisation du tourteau de soja en France à l’horizon 2005, par rapport à la période novembre 1999-octobre 2000. Cette évaluation est fondée sur les résultats de modélisation de formules alimentaires et les tendances de production d’aliments composés prolongées jusqu’en 2005. Plusieurs hypothèses ont alors été testées afin d’explorer différentes voies de reconquête de l’autonomie protéique : amélioration de la compétitivité des matières premières face au soja, amélioration de leur qualité nutritionnelle ou encore développement de systèmes d’élevage permettant de réduire la dépendance au soja. Ces réductions ont été estimées pour des voies telles que le remplacement du tourteau de soja par du tourteau de colza dans les rations fabriquées à la ferme dans les élevages laitiers ou porcins (750 000 t de tourteau de soja économisables. Dans le secteur des aliments composés, nos simulations ont montré l’importance de la thréonine de synthèse pour réduire l’utilisation de soja (350 000 t de soja économisables, tout comme l’intérêt d’une augmentation du niveau protéique des céréales (250 000 t de soja économisables pour une hausse d’un point du taux de protéines du blé.

  19. 多水平导师指导行为对员工结果的影响机制%Mechanisms Linking Multiple-level Mentoring and Protégés Outcomes

    Institute of Scientific and Technical Information of China (English)

    陈诚; 文鹏; 舒晓兵

    2015-01-01

    As an effective approach for employees' socialization and career development, mentoring has been widely used in various organizations. However, both outcomes and behaviors of mentoring are influenced by the changes from employee work needs and work style in organizations respectively. On the one hand, the previous mentoring research pay much attention to career outcomes of employees; On the other hand, the majority of mentoring research has only considered the dyadic mentor-protégé relationship and overlooks group-level mentoring. According to previous research, we firstly proposes a new concept named multiple-level mentoring behaviors, including providing group-level mentoring for all protégés and individual-level mentoring for one protégé. Moreover, this research examines the mediating role of self-efficacy and group identification in the relationships between multiple-level mentoring and protégés outcomes. And finally develops a cross-level modeling by examining the interaction of different level of mentoring on protégés outcomes. The present research will not only fill in the gaps in the mentoring outcomes (limited to career outcomes) and mentoring behaviors (neglecting group-level mentoring), but also give valuable implications for mentoring practice.%作为一种促进员工社会化和职业发展的工具,导师制已在各类企业中得到了广泛应用。然而,近些年员工工作需求以及组织工作方式的变化分别对导师指导行为的作用结果和指导内容产生了冲击。一方面,传统导师指导行为作用的研究大多限于员工职业结果;另一方面,传统导师制的研究仍停留在一对一的指导,忽略了针对徒弟群体的指导。为此,将在现实观察和理论回顾的基础上,首先提出多水平导师指导行为的概念,包括为徒弟群提供“一致性”指导的“群体水平指导行为”与为单个徒弟提供“差异化”指导的“个体水平指导行

  20. Demonstrating Hemostasis with a Student-Designed Prothrombin Time Test

    Science.gov (United States)

    Fardy, Richard Wiley

    1978-01-01

    Describes a blood coagulation test developed by two high school biology students. Although the test lacks some precision, results indicate that the technique is comparable to standard methods used in laboratories. (MA)

  1. 21 CFR 864.7750 - Prothrombin time test.

    Science.gov (United States)

    2010-04-01

    ... Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... the detection of possible clotting factor deficiencies in the extrinsic coagulation pathway, which involves the reaction between coagulation factors III and VII, and to monitor patients receiving coumarin...

  2. Efeito do níquel e da concentração protéica sobre o consumo e digestibilidade aparente da materia seca, proteína bruta, energia e balanço de nitrogênio do feno de Brachiaria brizantha cv. Marandu em ovinos Effect of nickel and protein concentration on consumption and apparent digestibility of dry matter, crude protein, energy, and nitrogen balance of a Brachiaria brizantha cv. Marandu hay, in ovines

    OpenAIRE

    O.J. De Paula; Graça,D.S.; Vasquez, E.A; MARTINS, R. G. R.

    2005-01-01

    Avaliaram-se os efeitos do níquel e da concentração protéica da dieta sobre o consumo e a digestibilidade aparente da matéria seca (MS), proteína bruta (PB) e energia, e balanço de nitrogênio do feno de Brachiaria brizantha cv. Marandu, oferecido a ovinos. As dietas testadas foram: A- feno; B- feno + níquel; C- feno + mistura protéica; D- feno + níquel + mistura protéica. As dietas continham, respectivamente 0,2, 5,2, 0,5 e 5,5ppm de níquel e 3,1, 3,1, 8,2 e 8,2% de PB. Nos níveis com menor c...

  3. L’océan au coeur de la Grande Île : Les aires marines protégées, un outil de développement durable pour Madagascar

    Directory of Open Access Journals (Sweden)

    Rémi A. Ratsimbazafy

    2011-06-01

    Full Text Available Grande Île de l’océan Indien, Madagascar apparaît comme une véritable « île continent » avec une superficie de plus de 590 000 km² et plus de 5000 km de côtes. Ses zones marines et côtières abritent une variété d’écosystèmes et d’habitats parmi lesquels les récifs coralliens, mangroves, dunes, lagons, plages sableuses, herbiers de phanérogames, estuaires, îles et îlots auxquels sont associés de nombreuses espèces de coraux, poissons, reptiles, crustacés, mollusques, échinodermes. Les écosystèmes les plus connus sont certainement les récifs coralliens avec une superficie de près de 5000 km² et les mangroves qui s’étendent sur plus de 3000 km² principalement sur les rivages occidentaux de l’île. Ils apportent d’énormes bénéfices socio-économiques aux communautés locales grâce à la pêche et aux activités touristiques sans oublier les services écologiques que ces écosystèmes assurent pour l’équilibre de la planète et le bien-être de l’Homme. Au vu de l’importance de ces zones marines et côtières et dans le but de les préserver face aux menaces et pressions d’origine anthropique et naturelle, Madagascar s’est résolument investie dans la création et la mise en place d’aires marines protégées et de réserves marines afin de contribuer au bien-être des communautés locales qui en dépendent. Dans cette optique, le Système d’Aires Protégées de Madagascar, SAPM, a été mis en place pour offrir les outils appropriés de gestion, de gouvernance et de planification dans l’objectif de tripler la surface des aires protégées, ce qui la porterait de 1,7 à près de 6 millions d’hectares (Vision Durban. La première aire marine protégée a été officiellement créée en 1989 avec le parc marin de Nosy Antafana intégré à la Réserve de Biosphère de Mananara Nord, suivie par les trois parcelles marines du Parc National de Masoala. Vingt ans après le premier parc marin

  4. Desenvolvimento e avaliação de um protótipo de cortador de base para colhedoras de cana-de-açúcar Development and evaluation of a prototype of base cutter for sugar-cane harvesters

    Directory of Open Access Journals (Sweden)

    Carlos E. S. Volpato

    2002-01-01

    Full Text Available Um protótipo de cortador de base para o seguimento pantográfico do perfil em colhedoras de cana-de-açúcar, foi projetado e construído e seu desempenho de flutuação foi avaliado em função da força normal de reação do perfil (F N. O trabalho foi conduzido em uma caixa de solo, o protótipo montado sobre um carro porta-ferramentas e submetido a ensaios nos quais variaram três tipos de perfil: senoidal, rampa ascendente e rampa descendente, duas amplitudes do perfil, 0,025 e 0,05 m e cinco velocidades de deslocamento: 0,69, 1,12, 1,48, 1,82 e 2,12 m s-1. Os melhores resultados foram verificados para os perfis em rampa, tanto ascendente quanto descendente, independentemente da amplitude do perfil e da velocidade de deslocamento e, também, para perfil senoidal de ambas as amplitudes, com velocidade de deslocamento inferior a 1,48 m s-1. Os resultados demonstraram que o protótipo ainda é passível de ajustes, uma vez que o desempenho de flutuação sobre o perfil pode ser melhorado através da utilização de técnicas de otimização.A prototype of base cutter for sugar-cane harvester was designed and built. The floating performance was evaluated as a function of the profile reaction force (F N. The prototype was tested in a soil box using a carrier car running on sinusoidal and ramp type profiles with amplitudes of 0.025 and 0.05 m. The travel speeds used were of five different levels: 0.69, 1.12, 1.48, 1.82 and 2.12 m s-1. The best results were obtained while running on ramp profiles, both ascending or descending, independent of the profile amplitude or the travel speed. The sinusoidal profile showed good results for the travel speed of 1.48 m s-1, for both the amplitudes tested. The results demonstrated that the prototype may still be adjusted, as the floating action on the profile can be improved through the use of optimization techniques.

  5. Desenvolvimento e caracterização de cereal matinal extrudado de mandioca enriquecido com concentrado protéico de soro de leite Development and characterization of an extruded breakfast cereal from cassava enriched with milk whey protein concentrate

    Directory of Open Access Journals (Sweden)

    Priscilla Andrade Silva

    2011-12-01

    Full Text Available O objetivo deste trabalho foi desenvolver e caracterizar um cereal matinal extrudado de mandioca enriquecido com concentrado protéico de soro de leite. O extrudado foi formulado adicionando-se 15% de concentrado protéico de soro de leite à farinha de mandioca. As matérias primas e o cereal matinal obtido foram analisados quanto às características físico-químicas. Para o cereal matinal desenvolvido, avaliaram-se também suas características microbiológicas e sensoriais. O extrudado estudado apresentou índice de expansão de 11,49 e densidade aparente de 0,21g.cm-3. Quanto à análise sensorial, o cereal matinal avaliado obteve notas médias de aceitação, para todos os atributos avaliados, situadas no intervalo de 7,63 a 8,05 que corresponde às categorias "gostei moderadamente" e "gostei muito". Para a intenção de compra, 91,70% dos provadores opinaram que certamente ou possivelmente comprariam o produto. O emprego de mandioca e concentrado protéico de soro de leite é uma alternativa para a elaboração de cereal matinal extrudado, obtendo-se um produto final com boas características sensoriais e nutricionais.The objective of this study was to develop and characterize an extruded cassava breakfast cereal enriched with milk whey protein concentrate. The extruded product was formulated by adding 15% of milk whey protein concentrate to cassava flour. The raw materials and the cassava flour breakfast cereal obtained were analyzed for their physicochemical characteristics. The breakfast cereal was also evaluated for its microbiological and sensory characteristics. The extruded product presented an expansion index of 11.49 and apparent density of 0.21g.cm-3. With respect to the sensory analysis, the breakfast cereal obtained hedonic ratings in the range from 7.63 to 8.05 for all the attributes evaluated, corresponding to the categories "I liked moderately" and "I liked a lot". For purchase intention, 91.70% of the panelists said they

  6. Role for non-proteolytic control of M-phase-promoting factor activity at M-phase exit.

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    Vincenzo D'Angiolella

    Full Text Available M-phase Promoting Factor (MPF; the cyclin B-cdk 1 complex is activated at M-phase onset by removal of inhibitory phosphorylation of cdk1 at thr-14 and tyr-15. At M-phase exit, MPF is destroyed by ubiquitin-dependent cyclin proteolysis. Thus, control of MPF activity via inhibitory phosphorylation is believed to be particularly crucial in regulating transition into, rather than out of, M-phase. Using the in vitro cell cycle system derived form Xenopus eggs, here we show, however, that inhibitory phosphorylation of cdk1 contributes to control MPF activity during M-phase exit. By sampling extracts at very short intervals during both meiotic and mitotic exit, we found that cyclin B1-associated cdk1 underwent transient inhibitory phosphorylation at tyr-15 and that cyclin B1-cdk1 activity fell more rapidly than the cyclin B1 content. Inhibitory phosphorylation of MPF correlated with phosphorylation changes of cdc25C, the MPF phosphatase, and physical interaction of cdk1 with wee1, the MPF kinase, during M-phase exit. MPF down-regulation required Ca(++/calmodulin-dependent kinase II (CaMKII and cAMP-dependent protein kinase (PKA activities at meiosis and mitosis exit, respectively. Treatment of M-phase extracts with a mutant cyclin B1-cdk1AF complex, refractory to inhibition by phosphorylation, impaired binding of the Anaphase Promoting Complex/Cyclosome (APC/C to its co-activator Cdc20 and altered M-phase exit. Thus, timely M-phase exit requires a tight coupling of proteolysis-dependent and proteolysis-independent mechanisms of MPF inactivation.

  7. BPH: a tell-tale sign of prostate cancer? Results from the Prostate Cancer and Environment Study (PROtEuS).

    Science.gov (United States)

    Boehm, Katharina; Valdivieso, Roger; Meskawi, Malek; Larcher, Alessandro; Sun, Maxine; Sosa, José; Blanc-Lapierre, Audrey; Weiss, Deborah; Graefen, Markus; Saad, Fred; Parent, Marie-Élise; Karakiewicz, Pierre I

    2015-12-01

    In a population-based case-control study (PROtEuS), we examined the association between prostate cancer (PCa) and (1) benign prostatic hypertrophy (BPH) history at any time prior to PCa diagnosis, (2) BPH-history reported at least 1 year prior to interview/diagnosis (index date) and (3) exposure to BPH-medications. Cases were 1933 men with incident prostate cancer diagnosed across Montreal French hospitals between 2005 and 2009. Population controls were 1994 men from the same age distribution and residential area. In-person interviews collected socio-demographic characteristics and medical history, e.g., BPH diagnosis, duration and treatment, as well as on PCa screening. Logistic regression analyses tested overall and grade-specific associations, including subgroup analyses with frequent PSA testing. A BPH-history was associated with an increased risk of PCa (OR 1.37 [95 % CI 1.16-2.61]), more pronounced for low-grade PCa (Gleason ≤6: OR 1.54 [1.26-1.87]; Gleason ≥7: OR 1.05 [0.86-1.27]). The association was not significant when BPH-history diagnosis was more than 1 year prior to index date, except for low-grade PCa (OR 1.29 [1.05-1.60]). Exposure to 5α reductase inhibitors (5α-RI) resulted in a decreased risk of overall PCa (OR 0.62 [0.42-0.92]), particularly for intermediate- to high-grade PCa (Gleason ≤6: OR 0.70 [0.43-1.14]; Gleason ≥7: OR 0.43 [0.26-0.72]). Adjusting for PSA testing frequency or restricting analyses to frequently screened subjects did not affect these results. BPH-history was associated with an increased PCa risk, which disappeared, when BPH-history did not include BPH diagnosis within the previous year. Our results also suggest that 5α-RI exposure exerts a protective effect on intermediate and high-grade PCa.

  8. Perda dentária e expectativa da reposição protética: estudo qualitativo Dental loss and prosthetic replacement expectation: qualitative study

    Directory of Open Access Journals (Sweden)

    Maria Elisa de Souza e Silva

    2010-05-01

    Full Text Available Esse estudo qualitativo analisou as repercussões da perda dentária total de usuários do serviço público. Aplicou-se o Oral Health Impact Profile simplificado(OHIP14 a cinquenta voluntários e, após a apuração dos resultados, foram selecionados treze pacientes para entrevistas individuais baseadas em um roteiro com questões abertas. A seleção das pessoas foi de acordo com o grau de percepção do impacto da condição bucal na qualidade de vida, medida pela pontuação OHIP, de forma a incluir a maior variedade de perfis da amostra inicial. Da análise do conteúdo dos depoimentos, verificou-se que a perda dentária total tem forte impacto na vida das pessoas e implica consequências negativas como vergonha, dificuldade em se alimentar, prejuízo ao relacionamento social e sentimento de incompletude. A possibilidade de reposição protética dos dentes gera ansiedade nas pessoas e, embora elas saibam que a utilização das próteses possa representar sacrifícios, a expectativa de retorno ao padrão social geralmente aceito e de recuperação de sua própria imagem rejuvenescida faz valer a pena quaisquer sacrifícios.This qualitative research analyses the repercussions of total dental loss on people's quality of life. The Oral Health Impact Profile short-form (OHIP14 was applied to 50 volunteers, and after analyzing the results, 13 out of those 50, were selected to be individually interviewed in an opened questions script. The criteria to select these 13 people were: perception of the impact of mouth condition on quality of life measured by the score reached on OHIP. We tried to comprise the sample with a good variety, according to the profile of the initial sample. After analyzing people's statements, it was possible to verify that dental loss had strong negative consequences on people's life, like shame, difficulty to eat, impact on social relationship and feeling of not being complete. The possibility of having their teeth replaced

  9. Digestibilidade de alimentos protéicos e energéticos para fêmeas de beta Digestibility of protein feedstuffs and energetic feedstuffs for female beta

    Directory of Open Access Journals (Sweden)

    Jener Alexandre Sampaio Zuanon

    2007-08-01

    Full Text Available Foram avaliados os coeficientes de digestibilidade aparente de MS, PB, energia bruta (EB e EE de alimentos protéicos (farelo de soja e farinha de peixe e energéticos (fubá de milho e farelo de trigo para beta (Betta splendens. Fêmeas adultas foram alojadas em gaiolas e mantidas em dois aquários de fibra de vidro (30 peixes/aquário para alimentação e dois para coleta de fezes, ambos de formato cônico e com capacidade para 30 L. Os resultados dos coeficientes de digestibilidade aparente de MS, PB, EB e EE foram, respectivamente, de 69,43; 72,52; 67,91 e 55,50% para farelo de soja; 60,67; 51,15; 75,55 e 58,26% para farinha de peixe; 63,88; 87,16; 77,61 e 50,40% para fubá de milho; e 61,06; 93,37; 58,17 e 65,51% para farelo de trigo. Os resultados obtidos neste estudo permitem otimizar a formulação de dietas práticas balanceadas, economicamente viáveis para a espécie.Apparent digestibility coefficients of dry matter, crude protein, gross energy and ether extract from protein feeds: soybean meal and fish meal and from energy feeds: corn and wheat middlings were evaluated for Siamese fighting fish (Betta splendens. Adult female fish were stocked in cages and held in two fiberglass aquaria (30 fish/aquarium for feeding and two aquaria for collect fecal samples, both with conic shape with 30L. The results of apparent digestibility coefficients for DM, CP, GE and EE were respectively 69.43, 72.52, 67.91 and 55.50% for soybean meal; 60.67, 51.15, 75.55 and 58.26% for fish meal; 63.88, 87.16, 77.61 and 50.40% for corn and 61.06, 93.37, 58.17 and 65.51% for wheat middlings. The determined digestibility values are essential to economically viable practical diet formulation for Siamese fish fighting.

  10. Fontes protéicas e energéticas com diferentes degradabilidades ruminais para novilhos de corte = Protein and Energy sources with differing degradabilities for finishing steers

    Directory of Open Access Journals (Sweden)

    Paola Ranzani Gabarra

    2007-04-01

    Full Text Available O objetivo deste trabalho foi determinar o efeito da sincronização dadegradação ruminal de fontes de amido (milho moído fino ou milho floculado e proteína (farelo de soja e uréia, no consumo de matéria seca, digestibilidade no trato total e parâmetros ruminais de novilhos Nelore em terminação. Quatro novilhos Nelore (300 kg PV foram utilizados em delineamento do tipo Quadrado Latino 4 x 4, fatorial 2 x 2: dois métodos de processamento do milho (moagem fina x floculação e duas fontes protéicas (farelo de soja x uréia. As rações continham 13% de feno de gramínea e 87% de concentrado. A floculação do milho reduziu a concentração de amido duodenal (p The objective of this study was to determine the effect of synchronization of starch (finely ground or steam-flaked corn and protein (soybean meal or urea rumen degradation on dry matter intake, total tract digestibility and rumen parameters of finishingNelore steers. Four Nelore steers (300 kg LW were utilized in a 4 x 4 Latin Square design, 2 x 2 factorial arragment: two corn processing methods (fine grinding vs. steam flaking and two protein sources (soybean meal vs. urea. The diets contained 13% grass hay and 87%concentrate. Steam flaking decreased (p < 0.01 starch concentration in duodenal digesta, which would explain lower ruminal pH (p < 0.15 and concentration of N-NH3 (p < 0.01, plasma urea-N concentration (p < 0.01; and the increased molar concentration of ruminal propionate (p < 0.01. As compared to fine grinding, steam-flaking corn increased(p < 0.01 total tract digestibility of starch (98.8 vs. 88.6%, but decreased (p < 0.01 NDF digestibility (41.9 vs. 12.1%. Protein sources had no effect on the evaluated variables. Increasing starch degradability through steam-flaking of corn improved dietary proteinutilization by beef steers.

  11. Manejo perioperatorio de sangre en cirugía protésica primaria de rodilla Perioperative blood management in primary total knee arthroplasty

    Directory of Open Access Journals (Sweden)

    E. Noain

    2005-08-01

    Full Text Available En cirugía protésica de rodilla la donación autóloga junto con la recuperación postoperatoria de sangre procedente del sistema de drenaje es una alternativa que puede ayudar a disminuir las necesidades de transfusión homóloga. Se han revisado 100 intervenciones de prótesis total primaria de rodilla realizadas en el Hospital García Orcoyen entre los años 2001-2003. Se emplearon el 88,7% de las bolsas de autotransfusión disponibles y, en los casos que se utilizó el recuperador sanguíneo, la media de reinfusión fue de 374 ml. (rango: 200-950 ml. En un 69% de los casos no fue necesaria la transfusión homóloga de sangre. Mientras se desarrollan otras técnicas hemoterápicas, creemos que la donación autóloga y la recuperación postoperatoria de sangre es eficaz en reducir las necesidades de transfusión homóloga lo cual adquiere importancia por el riesgo potencial de reacciones postransfusionales, transmisión de enfermedades y su reserva limitada en los bancos de sangre.Preoperative autologous blood collection and the reinfusion of postoperative blood salvaged from wound drainage can help to decrease the use of homologous blood transfusion in total knee arthroplasty. We have evaluated 100 primary total knee arthroplasties carried out at the García Orcoyen Hospital between 2001-2003. Eighty-eight point seven percent (88.7% of available autotransfusion blood packs were employed and the average blood reinfusion from wound drainage was 374 ml. (range: 200-950 ml when this method was possible. In 69% of cases homologous blood transfusion was not necessary. While other techniques are being developed, we think that autologous blood collection and blood reinfusion from wound drainage are effective in reducing the need for homologous blood transfusion; this is important because of the potential risk of reactions, disease transmission and limited blood bank resources.

  12. Avaliação da incidência das mutações G1691A no gene do fator V (fator V Leiden e G20210A no gene da protrombina em pacientes com diabetes mellitus tipo 2 Incidence of G1691A factor V gene (factor V Leiden and G20210A prothrombin gene mutations in type 2 diabetes patients

    Directory of Open Access Journals (Sweden)

    Anna L. Soares

    2005-09-01

    phenotype, which increases the risk of thrombotic events by a factor of seven. The G20210A mutation of the prothrombin gene results in hyperprothrombinemia, increasing the risk of thrombotic events by a multiple of three. These mutations are of great relevance considering that the presence of one or both can contribute to a hypercoagulability state accelerating complications in diabetes. The aim of this study was to evaluate the incidence of these mutations in controls (n=16, DM2 subjects (n=7, hypertensive subjects (HAS (n=12 and DM2+HAS subjects (n=18. The frequencies found were low and similar to those observed in the Brazilian population in general. It was not possible to establish any correlation among mutations and specific features of each group. It is still not clear if there is or not a higher prevalence of these mutations in diabetic individuals or if the mutation contributes to an increase in the risk of thrombotic events in these individuals. Further studies involving a larger number of patients are necessary in order to answer these questions.

  13. Desenvolvimento de um protótipo de software para geração de grade de programação de comerciais aplicável à TV Digital/IPTV utilizando Metaheurísticas

    OpenAIRE

    Brum, James Gladstone Fagundes

    2014-01-01

    Este trabalho apresenta o desenvolvimento de um protótipo de software, utilizando metaheurísticas por meio de um Algoritmo Memético, para a Geração de Grade de Programação de intervenções comerciais aplicada à TV Digital e a IPTV. O problema apresenta-se como uma linha de tempo na grade televisiva com sua programação onde estão definidos horários de intervenção em que grupos de comerciais devem ser exibidos. A organização destes comerciais nas intervenções obedecem a um conjunto de requisitos...

  14. Emprego de fubá de melhor qualidade protéica em farinhas mistas para produção de biscoito Use of quality-protein maize in flour blends and in the production of cookies

    Directory of Open Access Journals (Sweden)

    Fátima F. P. Guilherme

    2005-03-01

    Full Text Available Farinhas mistas (FM à base de farinha de trigo com substituição parcial por isolado protéico de soja, soro de leite em pó e três tipos de fubá (mimoso ou de cultivares BR451 e BR2121 foram preparadas para melhorar a qualidade protéica da farinha de trigo. As características sensoriais e químicas dos biscoitos foram avaliadas, e o valor protéico em potencial foi calculado. Os resultados foram comparados com os da farinha de trigo ou seu biscoito (controles. Os teores em macronutrientes nas FM e nos biscoitos foram superiores aos dos respectivos controles, com exceção de carboidratos. Apenas na aparência o biscoito com FM BR2121 foi estatisticamente inferior à daquele com FM mimoso, sendo semelhantes ao controle e aos outros biscoitos na textura e no sabor. O biscoito com FM mimoso teve a maior aceitação. O valor calórico médio dos biscoitos foi de 436kcal. A qualidade protéica dos biscoitos com as FM - lisina e treonina como aminoácidos limitantes e C-PER em torno de 2 - foi superior a do controle. O custo (preço/kg dos biscoitos assados com as FM foi superior ao do biscoito controle, devido ao custo da FM ter sido cerca do dobro da farinha de trigo pura e de se necessitar maiores quantidades de ingredientes na formulação.Flour blends (FB based on wheat flour with partial substitution for isolated soy protein, powdered whey and three corn flour types (common or cultivar BR451 and BR2121 were prepared to improve the protein quality of wheat flour. The sensory and chemical characteristics of the cookies were evaluated and the potential proteic value was calculated. The results were compared with the wheat flour or its cookie (controls. Macronutrients levels in FB and cookies were superior than those in the respective controls, except for carbohydrates. Only in the appearance the cookie made with FB BR2121 was statistically inferior to the one with common corn FB, being similar to the control and other cookies in texture and

  15. Aquisição e manipulação de imagens por tomografia computadorizada da região maxilofacial visando à obtenção de protótipos biomédicos

    OpenAIRE

    2008-01-01

    O processo de construção de protótipos biomédicos surgiu da união das tecnologias de prototipagem rápida e do diagnóstico por imagens. No entanto, este processo é complexo, em função da necessária interação entre as ciências biomédicas e a engenharia. Para que bons resultados sejam obtidos, especial atenção deve ser dispensada à aquisição das imagens por tomografia computadorizada e à manipulação dessas imagens em softwares específicos. Este artigo apresenta a experiência multidisciplinar de ...

  16. Avaliação da qualidade protéica de uma dieta estabelecida em Quissamã, Rio de Janeiro, adicionada ou não de multimistura e de pó de folha de mandioca

    OpenAIRE

    Boaventura,Gilson Teles; Chiappini,Claudete Corrêa de Jesus; ASSIS FERNANDES,Núbia Regina; OLIVEIRA,Elaine Martins de

    2000-01-01

    O trabalho avaliou a qualidade protéica da dieta consumida por crianças desnutridas do município de Quissamã, Rio de Janeiro, e adicionada da multimistura ou do pó de folha de mandioca, utilizando 48 Rattus norvergicus, Wistar, machos, com 24 dias, pesando 58,95 g, recebendo água e ração ad libitum. Na primeira fase, denominada de Desnutrição, os animais foram distribuídos em grupo controle, alimentados com ração à base de caseína, grupo Quissamã alimentados com ração à base da dieta estabele...

  17. Qualidade nutricional e escore químico de aminoácidos de diferentes fontes protéicas Nutritional quality and chemical score of amino acids from different protein sources

    Directory of Open Access Journals (Sweden)

    Christiano Vieira Pires

    2006-03-01

    Full Text Available As proteínas são moléculas essenciais para aos organismos animais, devendo, portanto, estar presentes na alimentação em quantidades adequadas. Além do aspecto quantitativo deve-se levar em conta o aspecto qualitativo, isto é, seu valor nutricional, que dependerá de sua composição, digestibilidade, biodisponibilidade de aminoácidos essenciais, ausência de toxicidade e de fatores antinutricionais. O objetivo deste trabalho foi avaliar a digestibilidade in vivo, o escore químico de aminoácidos (EQ e o escore químico de aminoácido corrigido pela digestibilidade protéica (PDCAAS das seguintes fontes de proteína: carne de rã sem osso, carne de rã com osso, carne de rã mecanicamente separada (CMS, carne bovina, ovo em pó, caseína, trigo, milho, soja convencional, soja isenta de inibidor de tripsina Kunitz e de lipoxigenases (soja KTI-LOX-, proteína texturizada de soja (PTS e feijão. As proteínas de origem animal apresentaram maiores valores de digestibilidade que as de origem vegetal. Carne de rã sem osso apresentou a proteína com maior digestibilidade protéica de todas as proteínas estudadas, não diferindo, entretanto, da caseína, CMS, carne bovina e rã com osso. Das proteínas de origem animal, a do ovo em pó foi aquela que apresentou menor digestibilidade protéica. Nenhuma das proteínas de origem animal apresentou aminoácidos essenciais limitantes quando comparadas com o padrão da FAO/WHO. Feijão, soja convencional, soja KTI-LOX- e PTS, tiveram os aminoácidos sulfurados (metionina+cisteína como limitantes. Enquanto que para trigo e milho, o aminoácido mais limitante foi a lisina. Soja KTI-LOX- e PTS apresentaram valores de PDCAAS superiores aos da soja convencional, mostrando uma possível elevação na qualidade protéica da soja melhorada geneticamente e da soja processada.Proteins are essential for animals, therefore, they must be present in diet, in appropriate amounts. Besides the quantitative aspect

  18. La traque des allergènes masqués dans l’alimentation. Exemples de l’extraction et de la détection immunochimique des protéines de l’arachide et du lait

    OpenAIRE

    Bernard, Herve

    2016-01-01

    La présence fortuite d’allergènes dans l’alimentation peut provoquer des réactions parfois sévères chez des individus sensibilisés. La détection de ces allergènes masqués dépend de la méthodologie développée, du marqueur protéique choisi et de la matrice alimentaire analysée. Différentes méthodologies ont été évaluées pour leur capacité à détecter des allergènes du lait et de l’arachide dans des matrices alimentaires complexes. Les conditions d’extraction ainsi que les tests ELISA appliqués i...

  19. Exigência protéica de juvenis de tambaqui (Colossoma macropomum após privação alimentar Protein requirement of tambaqui juveniles (Colossoma macropomum after feed deprivation

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    Luciana Santos

    2010-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a exigência protéica de juvenis de tambaqui (Colossoma macropomum após a privação alimentar. Os peixes (50,3 ± 0,26g foram submetidos a quatro rações isoenergéticas com diferentes concentrações protéicas (28, 32, 36 e 40% proteína bruta e dois regimes alimentares (com privação e sem privação durante 60 dias. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 4x2, em triplicata. Avaliaram-se os parâmetros de qualidade de água e de desempenho no 1º, 15º e 60º dia de experimento. Os parâmetros da qualidade da água permaneceram dentro dos padrões de cultivo. Não houve mortalidade nos tratamentos testados. Foram observados diferenças entre os tratamentos para os índices de desempenho analisados: taxa de crescimento específico, eficiência alimentar, consumo diário de ração, consumo de proteína bruta, taxa de eficiência protéica e ganho de peso relativo, com exceção do índice hepatossomático. Houve hiperfagia, indicando a existência de crescimento compensatório. Os peixes realimentados com 36% proteína bruta tiveram incremento no teor de gordura visceral e maior deposição de proteína corporal. Estes resultados explicam uma melhora nos dados de ganho de peso, duração da hiperfagia, taxa de crescimento e eficiência alimentar. A privação alimentar não influencia a exigência protéica em juvenis de tambaqui.The aim of this work was evaluate protein requirement of tambaqui (Colossoma macropomum juveniles after feed deprivation. During 60 days, fish (50,3 ± 0,26 g were fed on four isoenergetics diets with different protein content (28, 32, 36 and 40% crude protein at two feeding regimes (with and without feed deprivation under completely random factorial design (4x2 in triplicate. Water quality parameters and performance index were evaluated at 1º, 15º and 60º days. Water quality parameters were kept at normal fish cultivation patterns

  20. Drink fermented to the base of serum of milk and isolated soy protein / Bebida fermentada à base de soro de leite e isolado protéico de soja

    Directory of Open Access Journals (Sweden)

    Ivone Yurika Mizubuti

    2000-04-01

    Full Text Available The whey and soy protein were used as substrate to obtain a basic beverage, fermented by yogurt culture (Lactobacillus delbrueckii subsp. buigaricus / Streptococcus salivarius subsp. thermophilus YC-180 and by probiotic culture (Lactobacillus acidophilus La-5. The formulation ( whey 10g/100mL; soy protein 2,4 g/100mL; fiber 0,5 g/100mL and sucrose 15 g/100mL was fermented separately, and mixed (1:1 to obtain the final fermented product with 0.72% (lactic acid acidity, pH 4.3 and lactic acid bacteria count of 2,0 x 108 cfu/mL, adequate for production of the proposed beverage.O soro de leite e o isolado protéico de soja foram utilizados para obtenção de uma bebida base, fermentada com cultura de iogurte (Lactobacillus delbrueckii subsp. bulgaricus / Streptococcus salivarius subsp. thermophilus YC - 180 e cultura probiótica (Lactobacillus acidophilus La-5. A formulação (soro de leite 10 g/100mL; isolado protéico de soja 2,4 g/100mL; fibras 0,5 g/100mL e sacarose 15 g/100mL indicou que procedendo-se a fermentação seguida de mistura na proporção de 1:1, produziu-se um produto final com acidez de 0,72% de ácido láctico; pH 4,3 e contagem de bactérias lácticas de 2,0 x 108 ufc/mL adequado para a obtenção da bebida proposta.

  1. Action de I'AlA sur la teneur en azote total et protéinique des graines de céréales cultivées à different niveau de la capacité capillaire en eau

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    Wiesław Nowakowski

    2015-06-01

    Full Text Available L'action de I'AIA sur la teneur en azote total et en azote protéinique des graines du Triticum vulgare, d'Hordeum vulgare et d'Avena sativa cultivés a 30%, 60% et 90% de la capacité capillaire en eau du sable a tété étudiée au cours de trois ans (1969, 1970, 1971. La teneur en g-protéines totales liée au rendement des graines de céréales examinees a été plus élevée dans les conditions de sécheresse (30% de la capacite capillaire en eau à la suite d'un traitement auxinique. La teneur (% en azote total et proteinique ne semble pas etre tellement modifiee dans les graines de cereales à la suite d'un traitement auxinique.

  2. Respostas de frangos de corte fêmeas de duas linhagens a dietas com diferentes perfis protéicos ideais Responses of female broilers from two strain crosses to diets with differing ideal protein profiles

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    Sergio Luiz Vieira

    2007-12-01

    Full Text Available Foi conduzido um experimento com o objetivo de avaliar a formulação de dietas com diferentes perfis protéicos ideais para frangos de corte fêmeas. Foram usadas aves dos cruzamentos Cobb x Cobb 500 e Ross x Ross 308. As dietas foram fornecidas em um programa alimentar de quatro fases: 1 a 7, 7 a 21, 21 a 31 e 31 a 37 dias de idade. Até os 21 dias de idade, as aves receberam dietas com perfis protéicos ideais altos (A, médios (M e baixos (B. De 21 dias até o final do experimento, houve uma redistribuição dos tratamentos em que metade das aves recebendo dieta A passaram a receber dieta B e metade daquelas recebendo dieta B passaram a receber dieta A. As aves recebendo dieta M permaneceram com o mesmo perfil protéico por todo o período experimental. Foi utilizado um delineamento inteiramente casualizado em arranjo fatorial com três perfis protéicos e duas linhagens até os 21 dias de idade e cinco perfis protéicos ideais e duas linhagens dos 21 aos 37 dias de idade. As aves da linhagem Cobb apresentaram maior peso vivo aos 21 dias de idade e melhor conversão alimentar até o final do experimento, enquanto aves Ross apresentaram maior consumo de ração durante todo o período experimental. Ainda para frangos Ross, a proporção de carne de peito aos 31 dias de idade foi maior e a proporção de coxas + sobrecoxas, aos 31 e 37 dias de idade, menores em relação às aves Cobb. As aves recebendo as dietas A e M até os 21 dias de idade apresentaram respostas zootécnicas similares, ambas superiores às aves recebendo dieta B. A avaliação dos dados aos 31 e 37 dias de idade demonstrou que as aves do programa alimentar BB apresentaram peso vivo semelhante, maior consumo de ração e conversão alimentar pior na comparação com as aves dos demais tratamentos. Já na avaliação após o abate, as únicas diferenças observadas foram relativas à proporção de gordura abdominal aos 31 dias de idade. Foi possível concluir que aves Cobb

  3. Research on Multi-Objective Optimization Problem of PROT Project Assets Transfer Based on Matlab Genetic Algorithm%基于Matlab遗传算法的PRO T项目资产转让多目标优化问题研究

    Institute of Scientific and Technical Information of China (English)

    王艳伟; 黄宜; 程静; 李靖

    2015-01-01

    PROT项目融资资产转让关系到利益相关者的收益问题,也关系到项目融资系统整体的有序性。既要保证整体系统熵最小,还要使各利益相关方获得较大的收益,以保证系统整体目标的实现。因此,PROT项目资产转让是一个多目标优化问题。首先建立PROT项目资产转让多目标优化模型,然后借助Matlab遗传优化算法对PROT项目资产转让多目标优化模型进行求解。最后通过实例验证,取得了较好的效果。%The financing assets transfer of PROT project has significant effect on the benefits of stakeholders as well as on the order of the whole project financing system.In order to achieve the overall goal,the PROT project transfer of assets is a multi -objective optimization problem,which needs to ensure the smallest entropy of the whole system and much profit for all stakeholders at the same time.Firstly,a multi-objective optimization mod-el of PROT project assets transfer is established in this paper.With the adoption of Matlab tools,the multi-ob-jective optimization model of PROT project assets transfer is calculated.Finally,its effectiveness is verified through an example.

  4. Proposta de um software-protótipo para uso na assistência a pacientes com cateter central de inserção periférica (PICC Proposal for a software-prototype to assist patients with peripherally inserted central catheter (PICC

    Directory of Open Access Journals (Sweden)

    Luiz Célio Martins Freitas

    2017-04-01

    Full Text Available Objetivo: Propor um software-protótipo como estratégia para melhorar o processo de comunicação e os registros relativos ao cuidado de enfermagem a pacientes com cateter central de inserção periférica (PICC e descrever os passos para a construção de um protótipo de software. Método: Utilizou-se da Pesquisa de Intervenções, na modalidade de Processo de Intervenção e como método de operacionalização a Prototipação associados a instrumentos de informática. Resultado: Foi criado um modelo computadorizado de interface gráfica simples e projetado para uso em redes abertas ou fechadas. Conclusão: O desenvolvimento do software-protótipo para funcionar em rede web tornará o processo de implantação mais prático. Acrescenta-se que validação com equipe de saúde será realizada antes da utilização.

  5. Vein-Type Uranium Deposits in Proterozoic Rocks Les gisements uranifères de type filonien dans les terrains protérozoïques

    Directory of Open Access Journals (Sweden)

    Ferguson J.

    2006-11-01

    cette concentration dans le temps est réelle. Comme le développement de la croûte continentale depuis 2200 MA paraît en accord avec le principe unitariste (des causes actuelles, la seule variable qui pourrait expliquer la concentration de l'uranium dans des filons entre 2200 et 1700 MA semble être une évolution régulière de l'atmosphère. On suggère que l'hydrosphère était alors suffisamment oxydante pour déplacer l'UO2 mais que des conditions rapidement réductrices apparaissaient dès que l'on atteignait la lithosphère. Le phénomène réducteur provoquait la précipitation de l'uranium à partir de l'eau météorique dans les pièges structuraux appropriés, en grande partie formés pendant des phases d'érosion prolongées. Il se peut également que ces pièges structuraux aient été actifs au début de la sédimentation du Protérozoïc Moyen. Un développement rapide de cette couverture imperméable a préservé ces gisements d'uranium.

  6. 集群内师徒制企业间的创业传导--基于新塘羽绒产业集群的案例研究%Entrepreneurial Transmission among Mentor-Protégé Enterprises in Cluster:Case Study on Xintang Feather and Down Industrial Cluster

    Institute of Scientific and Technical Information of China (English)

    郑健壮; 靳雨涵

    2015-01-01

    Taking Xintang feather and down industrial cluster as the case study object ,this paper studies the entrepreneurial transmission rela‐tionship between 12 typical mentor‐protégé enterprises based on the interview to 24 enterprises in this industrial cluster.Three findings are sum‐marized as follows :firstly ,industrial cluster entrepreneurship is the entrepreneurship and entrepreneurial transmission which occur in the context of industrial cluster ,and take on the process of “mentor enterprise‐protégé enterprise(the later mentor enterprise)‐protégé enterprise” and transmit chronologically ;secondly ,entrepreneurial transmission incorporates entrepreneurial spillover ,entrepreneurial perception ,and entrepre‐neurial follow up ,and the entrepreneurial spillover from mentor enterprise influences protégé enterprise′s and industrial cluster′s entrepreneur‐ship both positively and negatively ;thirdly ,there is two transmission paths in mentor‐protégé enterprise entrepreneurship ,namely linear trans‐mission(vertical entrepreneurship and imitative entrepreneurship) and wave‐like entrepreneurship ,and most of paths are in the form of linear imitative entrepreneurship which lacks knowledge updating .%以新塘羽绒产业集群为案例研究对象,在对该集群的24家企业进行深度访谈的基础上,刻画了12家典型师徒制企业的创业传导关系。研究结论如下:集群创业就是在集群情境下发生的一种创业和创业传导现象,通常表现为“师傅—徒弟(师傅)—徒弟”的递进过程并呈时间分布特征;创业传导过程由创业溢出、创业感知和创业跟随组成,师傅企业的创业溢出对徒弟企业乃至集群创业具有正反两方面的巨大影响;集群内师徒制企业的典型创业传导路径有两种———线性传导(“连带创业”和“模仿创业”)和波浪式传导,且以线性传导的模仿创业为主,其知识更新较少。

  7. Efeitos da correção da acidose metabólica com bicarbonato de sódio sobre o catabolismo protéico na insuficiência renal crônica The effects of the correction of metabolic acidosis with sodium bicarbonate on protein catabolism in chronic kidney failure

    Directory of Open Access Journals (Sweden)

    Denise MAFRA

    2001-04-01

    Full Text Available A desnutrição protéico-energética constitui problema comum aos pacientes com insuficiência renal crônica, influenciando diretamente na sua morbi-mortalidade. A acidose metabólica tem papel no catabolismo protéico, ativando a via proteolítica proteasoma-ubiquitina, dependente de adenosina trifosfato, e conjuntamente com glicocorticóides induz uma maior atividade na desidrogenase que degrada os aminoácidos de cadeia ramificada. Esta revisão teve como objetivo descrever o mecanismo pelo qual a acidose metabólica nos pacientes com insuficiência renal crônica promove o catabolismo protéico, favorecendo assim a desnutrição, bem como avaliar os efeitos do uso de bicarbonato de sódio na correção da acidose e conseqüentemente redução do catabolismo protéico. Pesquisas mostram melhora da acidose pelo uso de bicarbonato de sódio e conseqüente redução do catabolismo protéico na insuficiência renal crônica, podendo ser esta uma conduta promissora na atenuação da desnutrição nestes pacientes.Protein-Energy Malnutrition is common among patients with chronic kidney failure, thus increasing morbidity and mortality. Several studies have shown that metabolic acidosis is a major cause of muscle protein breakdown, and recently it was attributed to ATP-dependent ubiquitin-proteasome proteolytic pathway. Acidosis, plus glucocorticoids, also respond to increasing branched-chain amino acids oxidation. In this review, the impact of metabolic acidosis on protein and amino acid metabolism is examined in order to understand its effect on lean body mass and the nutritional status of patients with chronic kidney failure. The study also observes whether or not sodium bicarbonate supplementation is beneficial to chronic kidney failure patients. In summary, there is a preliminary evidence suggesting that the correction of acidosis using sodium bicarbonate reduces protein degradation in chronic kidney failure patients, thus emerging as a

  8. Low prevalence of Factor V Leiden and the prothrombin G20210A mutation in a healthy population from the central-south region of Chile Baixa prevalência do Fator V Leiden e da mutação da protrombina G20210A em uma população sã da região centro-sul do Chile

    Directory of Open Access Journals (Sweden)

    Iván Palomo

    2009-01-01

    Full Text Available Thrombosis is a result of the interaction between predisposing genetic polymorphisms and acquired risk factors. The two prothrombotic polymorphisms which are most frequently associated with thrombosis are factor V (FV Leiden and the prothrombin (PT G20210A mutation. The objective of this work was to investigate the prevalence of both factors in the central-south region of Chile. Determination of the frequency was carried out by means of a genetic analysis of 1200 healthy, non-native individuals. The mutation of FV Leiden was found in 1.25% of the population and the PT G20210A mutation in 1.33%. None of the individuals were homozygosis or had both polymorphisms. The prevalences of FV Leiden and the PT G20210A mutation are less common in the healthy population.A trombose pode ser o resultado da interação de polimorfismo genético e fatores de riscos adquiridos. Os polimosfirmos protrombóticos mais frequentes são fator V (FV Leiden e a mutação da protrombina (PT G20210A. O objetivo deste trabalho foi investigar a prevalência de ambos os polimorfismos na região centro-sul do Chile. Foram realizadas análises genéticas (PCR RFLP de 1.200 pessoas saudáveis, não nativas da região. Foram encontrados 1,25% de mutação do Fator V Leiden e 1,33% da mutação da protrombina G20210A. Não foi detectada homozigose em ambos os polimorfismos. A prevalência de FV Leiden e da mutação G20210A é baixa na população estudada.

  9. The prothrombin induced by vitamin K absence or antagonist-Ⅱ as a good diagnostic factor in the HBV related BCLC 0-A stage hepatocellular carcinoma%PIVKA-Ⅱ诊断HBV相关早期肝癌的应用价值

    Institute of Scientific and Technical Information of China (English)

    孙燕妮; 王秀梅; 刘友德; 于吉广; 邹志强; 郭砚梅

    2016-01-01

    目的 评价维生素K缺乏或拮抗剂Ⅱ诱导的蛋白(PIVKA-Ⅱ)作为乙型肝炎病毒(HBV)相关早期肝癌患者诊断指标的效能.方法 采用PIVKA-Ⅱ和甲胎蛋白(AFP)检测试剂盒,分别对82例治疗前的HBV相关早期肝癌患者(A组),169例慢性乙型病毒性肝炎及其肝硬化患者(B组),68例HBV或者HBsAg携带者(C组)和138例健康者(D组)血清样本进行检测.运用受试者工作特性曲线(ROC)分析PIVKA-Ⅱ和AFP的诊断效能,并确定PIVKA-Ⅱ检测HBV相关早期肝癌的临界值.结果 A 、B、C、D组患者血清PIVKA-Ⅱ中位浓度分别为209.5、16、17和15 mAU/ml,AFP中位浓度分别为71.9、5.3、3.8和2.4 ng/ml,A组两项指标均显著高于其余3组,差异有统计学意义(x2=84.195,25.128;均P<0.01).血清PIVKA-Ⅱ检测肝癌的曲线下面积(AUC)为0.857(95% CI:0.804~0.913),优于AFP诊断肝癌的曲线下面积0.729 (95% CI:0.654~0.801)(P<0.01).PIVKA-Ⅱ检测HBV相关早期肝癌的最佳临界值为26.5 mAU/ml.PIVKA-Ⅱ和AFP联合检测,可提升肝癌诊断的灵敏度、特异度、阴性预测值和阳性预测值.结论 血清PIVKA-Ⅱ对于HBV相关早期肝癌的诊断效能优于AFP,有望作为肝癌早期筛查的血清标志物应用于临床,为肝癌的早诊早治提供帮助.%Objective To assess the diagnostic value of the prothrombin induced by vitamin K absence or antagonist-Ⅱ (PIVKA-Ⅱ) in Chinese patients with hepatitis B virus (HBV) related hepatocellular carcinoma (HCC).Methods The study group consisted of 82 HCC patients [group A,Barcelona Clinic Liver Cancer (BCLC) 0-A stage],169 patients with chronic hepatitis B or HBV-related liver cirrhosis (group B),68 HBV or hepatitis B surface antigen (HBsAg) carrier patients (group C) and 138 healthy controls (group D).All patients and controls were examined for serum levels of PIVKA-Ⅱ and alpha-fetoprotein (AFP).Results The median serum levels of PIVKA-Ⅱ in groups A,B,C and D were 209.5 mAU/ml,16 m

  10. Estimativa do teor de fenilalanina em sopas desidratadas instantâneas: importância do nitrogênio de origem não-protéica Estimation of phenylalanine (Phe contents in dehydrated soups: significance of non-protein nitrogen

    Directory of Open Access Journals (Sweden)

    Claudia Passos Guimarães

    2005-09-01

    Full Text Available A análise direta de fenilalanina (Phe em alimentos com reduzidos teores protéicos destinados a pacientes fenilcetonúricos é difícil, demorada e de alto custo. Emprega-se, geralmente, método indireto baseado na análise do teor protéico bruto, considerando que proteínas naturais contêm ao redor de 4% de Phe. Neste trabalho estimou-se a concentração de Phe em 22 amostras de sopas desidratadas instantâneas, considerando-se os teores de N total, N protéico e não-protéico e de glutamato monossódico (GMS, que foram analisados independentemente. A concentração de proteína bruta (N total multiplicado por fator de conversão adequado variou entre 6,1 e 21,5 g/100 g amostra, apresentando similaridade com os valores protéicos apresentados nos rótulos das sopas. Os teores protéicos reais foram reduzidos e a concentração de Phe calculada a partir desses dados variou entre 51 e 652 mg/100 g amostra. A concentração de GMS nessas amostras foi considerável, sendo que o N proveniente desse realçador de sabor contribuiu com 2,5 a 47,7% do N total. Concluiu-se que, devido à elevada participação de GMS na formulação das sopas, a estimativa de Phe deve ser precedida de extração prévia de substâncias nitrogenadas não protéicas, para evitar superestimação da proteína e, por conseqüência, dos teores de Phe. As sopas desidratadas com os menores teores de Phe podem representar uma diversificação no cardápio para fenilcetonúricos.The direct analysis of phenylalanine (Phe in food with low protein content is difficult, time consuming and expensive. In general, more convenient indirect methods based on raw protein contents have been used, considering that natural proteins contain about 4% of Phe. In this paper the Phe contents of 22 commercial dehydrated soups were estimated taking into account the contents of total N, protein N and non-protein nitrogen as well as the contents of monosodium glutamate (GMS which were analyzed

  11. Desnutrição energético-protéica e cárie dentária na primeira infância Protein-energy malnutrition and early childhood caries

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    Dijane Pereira Costa

    2010-02-01

    Full Text Available Este estudo tem por objetivo investigar a relação existente entre a desnutrição energético-protéica e a cárie dentária precoce na primeira infância. Trata-se de uma comunicação, para a qual foi realizada uma revisão da literatura, a partir de análise documental de produção bibliográfica, baseada em levantamentos de periódicos e consultas a livros. Verificou-se que a cárie precoce na infância é altamente prevalente nas comunidades de baixa renda, nas quais a desnutrição é um fator comum e de grande relevância. Estudos mostram que crianças desnutridas tendem a apresentar defeitos estruturais no esmalte do dente, como também estão predispostas a um maior risco à cárie dentária. Portanto, uma deficiência energético-protéica durante a fase de desenvolvimento dentário (odontogênese tem demonstrado uma maior suscetibilidade à cárie dentária, atraso na cronologia de erupção e defeitos estruturais do esmalte (hipoplasia de esmalte, hipofunção das glândulas salivares e mudança na composição da saliva. Estes fatores podem ser os mecanismos pelos quais a desnutrição associa-se à cárie. Em conclusão, os estudos sugerem que a deficiência energético-protéica durante a odontogênese gera atraso na cronologia de erupção e defeitos estruturais no esmalte (hipoplasia, além de poder afetar as glândulas salivares, aumentando o risco de ocorrência da doença cárie. Estudos investigando associações entre desnutrição e cárie na primeira infância são de grande relevância para ampliar o conhecimento desta doença e o desenvolvimento de ações de promoção e de prevenção desse problema de saúde pública com aplicações nas áreas de nutrição e odontologia.This study aimed to investigate the relationship between protein-energy malnutrition and early childhood caries. For this communication, a review of the literature was performed, based on documental analysis of bibliographic searches, surveys of

  12. Concentrados protéicos para bovinos: 1. Digestibilidade in situ da matéria seca e da proteína bruta Protein concentrates for bovines: 1. In situ digestibility of dry matter and crude protein

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    J.F.C. Moreira

    2003-06-01

    Full Text Available O objetivo deste trabalho foi determinar a degradação ruminal, pela técnica in situ, da matéria seca (MS e da proteína bruta (PB de 10 concentrados protéicos. As degradações potenciais da MS e da PB das farinhas de origem vegetal, soja, algodão, mamona e palmiste, mostraram-se elevadas (próximas a 100%, porém em função de taxas de degradação mais altas para o farelo de soja (10% e menores para o algodão (4%, mamona (MS:3%; PB:1,2% e palmiste (1,7%, as degradabilidades efetivas (DE foram bem superiores para o farelo de soja, independentemente da taxa de passagem, o que a torna a fonte protéica de maior disponibilidade ruminal. O glúten de milho mostrou ser uma fonte protéica de baixa degradabilidade ruminal (DE da PB: 16% para 0,05 de taxa de passagem. Dentre os alimentos de origem animal, a maior degradabilidade potencial da proteína bruta foi verificada para a farinha de carne e ossos ( 75,5%, seguida das farinhas de peixe I (58,5%, de penas e vísceras (52,3% e de sangue (36,7%. A maior degradabilidade efetiva para a taxa de passagem de 5% foi a da farinha de carne e ossos (51%, seguida da farinha de peixe I (41%, de penas e vísceras (40,0% e de sangue (33%. A farinha de peixe II apresentou valores muito baixos de degradabilidade, apenas 22% com 48h de incubação ruminal.This work aimed to determine the in situ rumen digestibility of dry matter (DM and crude protein (CP using 10 protein sources. Potential degradabilities of DM and CP of soybean meal, cottonseed meal, castorbean meal and palmist coconut meal were high (around 100%, however due to higher fractional degradation rates of soybean meal (10% and lower for cottonseed meal (4%, castorbean meal (DM:3%, CP:1.2% and palmist meal (1.7%, the effective degradability (ED of soybean was significantly higher despite the rates of passage, showing this protein source has the highest ruminal nitrogen availability (from 86% to 63%, as rate of passage increased from 2 to 8

  13. Effect of foliar application of a protein hydrolysate in the productivity of broccoli cultivars. = Efeito da aplicação foliar de hidrolisado protéico sob a produtividade de cultivares de brócolis.

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    Marcelle Michelotti Bettoni

    2013-08-01

    Full Text Available - The broccoli (Brassica oleracea var. Italica is a Brassica vegetable grown in various regions of the world and is characteristic of small properties with potential for organic crops. However, surveys that provide products that meet organic legislation, biofertilizers as the basis of protein hydrolysates are necessary for legal purposes. The objective of this study was to evaluate the effect of protein hydrolysate containing amino acids on the production of two cultivars of broccoli grown in organic cropping system. The treatments were arranged in a completely randomized factorial 2 x 4 with four replicates, two cultivars (Belstar and Fiesta and four treatments: control and foliar application of solutions with the following concentrations of protein hydrolysate: 2 mL L -1, 4 mL L-1, 8 mL L-1. The applications were made weekly beginning and ending at 7 to 63 days after transplant (DAT and evaluated the following characteristics at harvest (75 DAT: fresh mass per head (FMH, dry mass per head (DMH, head diameter (HD and estimated average production (EAP. The use of hydrolyzed in 8 mL L-1dose resulted in higher fresh and dry mass, head diameter and yield of broccoli in organic cropping system. = - O brócolis (Brassica oleracea var. italica é uma hortaliça da família das Brássicas cultivado em diversas regiões do mundo, característico de pequenas propriedades, com potencial para cultivos orgânicos, porém é necessário que sejam realizadas pesquisas que disponibilizem produtos que atendam a legislação orgânica, como biofertilizantes a base de hidrolisados protéicos. Objetivou-se com este trabalho avaliar o efeito da aplicação de hidrolisado protéico contendo aminoácidos na produção de duas cultivares de brócolis, em sistema de cultivo orgânico. Os tratamentos foram dispostos em delineamento inteiramente casualizado, em esquema fatorial 2 x 4, com quatro repetições, sendo duas cultivares (Belstar e Fiesta e quatro tratamentos

  14. Avaliação de dietas com diferentes fontes protéicas para cães adultos Evaluation of diets with different protein sources for adult dogs

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    Aulus Cavalieri Carciofi

    2006-06-01

    Full Text Available Foram comparados os coeficientes de digestibilidade aparente (CDA de quatro alimentos secos extrusados para cães, cada um formulado com um dos ingredientes protéicos em estudo: farelo de soja (FS; farelo de glúten de milho (GM; farinha de carne e ossos (FCO; e farinha de vísceras de frango (FV. O experimento foi realizado em delineamento inteiramente casualizado, com quatro tratamentos (ingredientes protéicos e seis repetições, totalizando 24 animais. As médias dos tratamentos foram comparadas pelo teste Tukey. O CDA da PB (média ± erro-padrão da média foi maior na ração à base de GM (88,13±0,40%, seguida pelas dietas com FS (86,31±0,34%, FCO (85,88±0,16% e FV (84,84±0,15%. O CDA da MS foi maior para a ração com FV (83,69±0,09%, intermediário para GM (82,41±0,23% e FCO (82,76±0,11% e menor para FS (81,10±0,16%. As rações à base de proteína animal apresentaram os maiores CDA dos extrativos não-nitrogenados. O teor de MS das fezes dos cães foi elevado na ração com FCO, intermediário naquela com FV e GM e baixo naquela à base de FS. As quatro fontes protéicas estudadas apresentaram bons CDA e, portanto, podem ser utilizadas em rações para cães adultos.The coefficient of apparent digestibility (CAD of four dry extruded dog foods was compared, each one formulated with a different protein source: soybean meal (SM, corn gluten meal (GM, meat and bone meal (MBM and poultry meal (PM. A completely randomized design was used, with four treatments (protein source and six repetitions per treatment, in a total of 24 animals. Tukey test was used to compare treatments means. The diet based on GM showed the highest CAD of crude protein (88.13±0.40%, followed by SM (86.31±0.34%, MBM (85.88±0.16%, and PM (83.69±0.09%, with the lowest results. The CAD of dry matter was highest for PM (83.69±0.09%, intermediary for GM (82.41±0.23% and MBM (82.76±0.11%, and lowest for SM (81.10±0.16% The rations based on animal protein

  15. Suscetibilidade a antifúngicos de cepas de Candida albicans isoladas de pacientes com estomatite protética Susceptibility to antifungal drugs of Candida albicans strains isolated from patients with denture stomatitis

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    Jéssica Moreira BATISTA

    1999-12-01

    Full Text Available Pacientes portadores de próteses totais, apresentam, com freqüência a chamada estomatite protética, com a qual associa-se Candida albicans determinando a chamada candidíase eritematosa. Assim, procuramos avaliar a suscetibilidade dessa levedura a agentes antifúngicos. A suscetibilidade de dezenove cepas de Candida albicans isoladas de pacientes apresentando estomatite protética foi estudada frente a: um derivado poliênico representado, pela anfotericina B (AnB, e dois derivados azóicos, cetoconazol e miconazol. A atividade antifúngica foi estudada a partir da determinação da concentração inibitória mínima (CIM e da concentração fungicida mínima (CFM, pela técnica de diluição em ágar. Os resultados obtidos, mostraram baixos valores de CIMs e CFMs (mg/ml para a AnB frente a todas as leveduras. Para o miconazol e o cetoconazol, foram observadas CIMs invariavelmente £ 4,00 mg/ml; para as CFMs, foram obtidos valores ³ 16,00 mg/ml frente a maioria das cepas. Conclui-se que a AnB apresentou maior ação fungicida in vitro enquanto os azóis demonstraram ação fungistática mas não fungicida. Acreditamos que a pesquisa de novas drogas, principalmente de uso tópico ainda é necessária, a fim de se tratar, com sucesso, a candidíase eritematosa, comumente observada nas chamadas estomatites protéticas.Users of total prosthesis present in general a high frequency of the so called denture stomatitis, associated to erythematous candidiasis. So, we evaluated the susceptibility of oral yeast to three antifungal agents. Strains of Candida albicans isolated from patients with denture stomatitis were evaluated in relation to the susceptibility of the antifungal drugs as amphotericin B (polyenic derivatives, and azole agents as ketoconazole and miconazole. The antifungal activity was evaluated, and the minimal inhibitory concentration (MIC and minimal fungicide concentration (MFC were determined utilizing the agar dilution method. The

  16. Avaliação protéica de uma nova multimistura com base no milho QPM BR 473 Protein evaluation of a nutritional supplement based on QPM BR 473 maize

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    Enara Cristina Silva Glória

    2004-09-01

    Full Text Available A multimistura tem sido utilizada no Brasil pela Pastoral da Criança, em parceria com governos municipais, a fim de reduzir a desnutrição infantil. Não obstante, a eficácia deste suplemento tem sido constantemente arguida, devido à possível presença de fatores antinutricionais. No presente trabalho descrevemos a avaliação biológica de um suplemento contendo milho QPM BR473. Trinta e seis ratos Wistar machos, com 21-23 dias de idade, foram divididos em seis grupos de seis animais cada e alimentados com dietas de caseína contendo multimistura pura, com QPM BR473, láctea (contendo leite em pó, láctea contendo QPM BR473 ou a multimistura proposta (contendo QPM BR473, farinhas de aveia, soja e banana e açúcar mascavo. Mediu-se a Retenção Protéica Líquida. A condição microbiológica dos suplementos e seu custo foram também determinados. Os resultados obtidos mostraram que o QPM BR473 pode ser usado em suplementos nutricionais, com alto valor nutritivo, expresso por sua qualidade protéica, e com baixa relação custo/benefício.Nutritional supplements, known as "multimisturas", prepared with low cost ingredients have been distributed in Brazil by municipal governments, in partnership with non-governmental organizations, in order to reduce infant malnutrition. Nevertheless the efficacy of these supplements has been constantly argued, due to the possible presence of anti-nutritional factors. The present work describes the biological evaluation of a supplement containing Quality Protein Maize BR 473. Thirty six 21-23-day old male Wistar rats were divided into six groups of six animals each. The groups were fed casein diets, each containing respectively: "multimistura", "multimistura" with QPM BR 473, "multimistura" with powdered milk, "multimistura" with powderedmilk and QPM BR 473 or the proposed new supplement (containing QPM BR 473 flour, oat meal, soybean flour, brown sugar and banana meal. Net protein retention was measured

  17. Functional Foods: a new frontier for developing whey based protein beveragesAlimentos funcionais: uma nova fronteira para o desenvolvimento de bebidas protéicas a base de soro de leite

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    Ana Lúcia Barretto Penna

    2011-10-01

    Full Text Available Functional foods are defined as those that provide additional benefits to consumers. The market for foods with functional properties is expanding, supported by scientific evidences, which leads the developing of different special foods. This market segment is related to the innovations, as well as the traditional functional dairy and non dairy products, gain traction in the marketplace for innovative products like based whey sports beverage. Besides the functionality of muscle protein synthesis, these beverages are entering into areas such as clinical nutrition and satiety. Thus, it can be supposed that the composition and technological versatility of the whey support the use as innovative ingredient for foods and beverages. This review aim to supply an overview about functional foods, mainly the segment of whey based beverages. The success of this new nutritional approach is close related to the requirement to identify, to characterize and to develop methodologies to measure and to validate more precisely the relevant functional markers, making them open for a public domain.Alimentos funcionais são definidos como aqueles que fornecem benefícios adicionais aos da alimentação. O mercado de alimentos com propriedades funcionais está em crescente expansão, apoiado por evidências científicas, que creditam o desenvolvimento de alimentos diferenciados. Esse segmento está relacionado às inovações, pois além dos tradicionais produtos funcionais lácteos e não lácteos, ganham espaço no mercado produtos inovadores como bebidas especiais para esportistas a base de soro de leite. Além da funcionalidade sobre a síntese protéica muscular, estas bebidas protéicas estão se inserindo em áreas como a da saciedade e da nutrição clínica. Assim, é possível afirmar que o soro de leite se diferencia como ingrediente de inovação em alimentos e bebidas, porque demonstra uma grande versatilidade tecnológica. Esta revisão objetiva fornecer

  18. Caracterização de concentrado protéico de folhas de mandioca obtido por precipitação com calor e ácido Characterization of cassava leaf protein concentrate obtained by heat and acid precipitation

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    Cláudia de Fátima Modesti

    2007-09-01

    Full Text Available As folhas de mandioca apresentam um teor elevado de proteínas, todavia sua digestibilidade é baixa. A produção de concentrado protéico de folhas de mandioca (CPFM permite a utilização das proteínas das folhas com um reduzido teor de fibras e melhor qualidade protéica. Neste trabalho, analisaram-se características químicas de CPFM obtidos por diferentes formas de precipitação, com calor e com ácido. Os CPFM praticamente não apresentaram diferenças na composição centesimal. O nível de proteína dos CPFM aumentou 57,72% em comparação ao da farinha de folhas de mandioca (FFM. Os rendimentos de extração das proteínas também foram semelhantes para os CPFM. O teor de Fe dos CPFM foi mais elevado quando comparado com o da FFM. A FFM apresentou absorção de água e de óleo mais elevada que os CPFM, mas, entre os tipos de CPFM, os resultados foram semelhantes. A mínima solubilidade de nitrogênio da FFM e dos CPFM foi observada em pH entre 3 e 5. Verificou-se que a FFM apresentou uma capacidade de formação e estabilidade de espuma mais elevada que os CPFM. Tanto a FFM quanto os CPFM não apresentaram boa estabilidade de emulsão.Cassava leaves have a high content of protein of low digestibility. The production of cassava leaf protein concentrate (CLPC enables cassava leaf protein to be used with a reduced fiber content and better protein quality. This work involved an investigation of the chemical characteristics of CLPC produced by different forms of precipitation, using heat and acid. No significant differences were found in the centesimal composition of the CLPC. The protein content in CLPC was 57.72% higher than in cassava leaf flour (CLF. Both types of CLPC precipitation also yielded similar protein concentrations. The CLPC Fe content was higher than that of the CLF. The CLF presented higher water and oil absorption than did CLPC, although the CLPC extracted by the two methods showed similar results. The lowest nitrogen

  19. Perfil de utilização de repositores protéicos nas academias de Belém, Pará Pattern of utilization of protein supplements in academies in Belém, Pará

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    Ana Cláudia Matos de Araújo

    1999-04-01

    Full Text Available Este estudo foi realizado no primeiro semestre de 1996 e teve como alvo os freqüentadores das academias da cidade de Belém (PA. Além de pesquisa bibliográfica referente ao metabolismo energético e protéico, necessidades nutricionais de desportistas e suplementação com proteínas e aminoácidos, foi realizada uma pesquisa de campo com o objetivo de traçar o perfil de utilização de suplementos nutricionais, com ênfase em produtos a base de proteínas e aminoácidos, por sexo, idade e tempo de prática de atividade física. A pesquisa de campo envolveu uma amostra de 18 academias e 388 entrevistados, dos quais 103 (27% faziam uso de algum tipo de suplemento, sendo que 45 destes (44% utilizavam repositores protéicos. Das justificativas para opção de uso desses suplementos, destacou-se a "indicação profissional" que comparada ao quadro técnico existente nessas unidades, em que apenas 4 das 18 academias pesquisadas apresentavam nutricionistas ou médicos, indica que a utilização desses produtos por parte dos praticantes de atividades físicas está sendo indevida.The presente study was carried out in the first semester of 1996, aiming to evaluate users of academies in Belém, Pará, Brazil. Beyond vast bibliographic research about the energetic and proteic metabolism, sportsmen's nutritional necessities and supplementation with protein and amino acids, a field research was carried out with the purpose of tracing the profile of utilization of nutritional suplements with emphasis on protein and amino acid products, considering sex, age and period of physical activity practice. The research involved a sample of 18 academies and 388 interviewees; 103 of then (27% used some kind of supplementation and 45 of these (44% used protein supplements. Among the justifications given for the option of using the supplementation, the "professional indication" stood out from the others. Comparison with technical list existing in these units, in

  20. Protocolo protésico - quirúrgico en temporización inmediata de dientes unitarios, utilizando una cubeta multifuncional desmontable Prosthetic protocol - surgery in the immediate temporization of a single tooth using a multifunctional removable tray

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    Y Leighton

    2010-04-01

    Full Text Available Propósito: El presente reporte clínico es la descripción de un protocolo protésico - quirúrgico que permite la visualización directa del sitio operatorio y el correcto posicionamiento protésico - implantario de una corona temporal atornillada, construida inmediatamente de insertado el implante. Material y métodos: Se seleccionaron 10 pacientes del programa de especialización en implantologia bucomaxilofacial de la Universidad de Chile, cuyo criterio de inclusión principal fue ser desdentado parcial unitario posterior, con antagonista dentario natural. Como protocolo diagnóstico se solicitaron exámenes de laboratorio, exámenes radiograficos y se registraron sus arcos dentarios con impresiones de estudio a fin de obtener modelos de yeso, los que fueron posicionados en un articulador semiajustable tipo Whip Mix, donde se confeccionó un encerado diagnóstico del diente a reemplazar, libre de contacto oclusal en máxima intercuspidación (MIC y en los movimientos excursivos mandibulares. Basado en el encerado de diagnóstico se diseño una cubeta multifuncional desmontable (U. de Chile cuya característica fundamental es que fusiona una guía quirúrgica y una guía de montaje protésico, para una correcta temporización inmediata. El procedimiento se inicia mediante un colgajo de espesor total, posicionamiento en boca de la guía multifuncional en fase quirúrgica, para permitir el fresado del lecho implantario y la inserción implantaria. El lecho quirúrgico es suturado mediante una seda negra trensada y se confecciona en forma inmediata una corona temporal atornillada utilizando la guía multifuncional en fase de carga protésica. Resultados: Se instalaron un total de 12 implantes Renova Tapered (Lifecore utilizando la cubeta multifuncional la cual facilitó la direción del fresado y la contrucción de sus respectivas coronas temporales de resina acrilica, no segmentadas atornilladas. Conclusiones: Con las limitaciones de este

  1. Influencia del medio aromático en la transferencia del protón y determinación de los mínimos de potencial de algunas uniones 0-H ...N

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    Oscar Osorno R.

    2009-07-01

    Full Text Available Los complejos formados por unión hidrógeno en los sistemas 2,4,6 -Triclorofenol-Trietilamina y Pentaclorofenol-Trietilamina forma pares iónicos cuando se encuentran en un medio aromático y complejos normales cuando se encuentran en un medio no aromático como el ciciohexano y el tetracloroetileno. El estudio dieléctrico y de espectroscopia U.V. han permitido obtener los momentos dipolares de las formas tautoméricas en equilibrio y sus fracciones molares, tanto en los solventes puros como en mezclas benceno-ciclohexano. Por medio de las medidas calorimétricas efectuadas, se obtienen las entalpias de complejación para los sistemas en benceno y tetracloroetileno y a partir de éstas, es posible determinar los calores de formación del complejo normal y del par iónico. Utilizando una función potencial adecuada para la unión 0-H N, se deduce la distancia O N y la posición de equilibrio del protón en la unión, las cuales reproducen las entalpias de formación halladas experimentalmente.

  2. Rehabilitación protésica de un niño de 3 años con Displasia ectodérmica hipohidrótica

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    Maura María-Márquez-Junco

    2012-01-01

    Full Text Available La Displasia Ectodérmica Hipohidrótica (DEH o síndrome de Christ-Siemens-Touraine, es una rara enfermedad de carácter congénito, siendo afectado uno o varios componentes del tejido ectodérmico. Caracterizado por hipohidrosis, hipotricosis e hipodoncia. La mayoría de los casos se relaciona con una herencia recesiva ligada al cromosoma X, afectándose por consiguiente en los varones, pero también existen otras formas de herencia autosómica dominante y recesiva. Se presenta el caso clínico de un niño de 3 años 5 meses con oligodoncia y reabsorción de los procesos alveolares por ausencia total de las piezas deciduas, por lo que se realiza la rehabilitación protésica con prótesis total removible, como resultado se logra aumento de la dimensión vertical, mejora de la fonación, masticación, habla y la autoestima del paciente.

  3. Addition of proteic nitrogen during alcoholic fermentation for the production of cachaça Adição de nitrogênio protéico durante a fermentação alcoólica de caldo de cana para produção de cachaça

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    Elisangela Marques Jeronimo

    2008-04-01

    Full Text Available Cachaça is the denomination of a typical and exclusive Brazilian spirit produced from the distillation of fermented sugarcane juice must. The objective of this study was to evaluate the effect of adding yeast extract to the sugarcane juice used for sugarcane liquor production, because for the artisanal process no studies are available on nitrogen addition nor beverage quality, involving nitrogen complementation. Results of previous studies in the laboratory scale showed that sugarcane juice complementation with proteic nitrogen can be a beneficial practice for yeast multiplication and cellular growth, and also for the improvement of fermentation yield and liquor productivity. In this pilot scale study, using recycled yeast, the addition of proteic nitrogen influenced positively the cell viability, confirmed the yeast recycling operation, and also reduced the fermentation time. The proteic nitrogen addition did not affect the sensory acceptance of the distillate, and did not change the contents of volatile compounds, indicating that assimilable forms of proteic nitrogen can be helpfull to improve the alcoholic fermentation for cachaça production.Cachaça é a denominação de uma típica e exclusiva bebida destilada brasileira produzida a partir da destilação do caldo fermentado da cana-de-açúcar. O objetivo deste estudo foi avaliar a adição de extrato de levedura no mosto de caldo de cana para a produção de cachaça, pois em processo artesanal não há estudos específicos sobre as características fermentativas da levedura assim como sobre a qualidade da bebida, envolvendo a complementação nitrogenada e em específico a aplicação de nitrogênio protéico. Os resultados obtidos em trabalhos anteriores, conduzidos em laboratório, indicaram que nas destilarias artesanais de aguardente a complementação protéica do mosto pode constituir uma prática benéfica para a multiplicação e crescimento celular do fermento e conseq

  4. Composição centesimal e valor protéico de levedura residual da fermentação etanólica e de seus derivados Centesimal composition and protein nutritive value of yeast from ethanol fermentation and of yeast derivatives

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    Eunice Akemi Yamada

    2003-12-01

    Full Text Available Este trabalho teve por objetivo promover a autólise e o fracionamento da levedura (Saccharomyces sp. para produção de autolisado e extrato, bem como para produção de concentrado protéico fosforilado, a partir da levedura residual das destilarias de álcool etílico. Foram estudados a composição centesimal, o perfil de aminoácidos essenciais e o valor protéico dos três derivados comparativamente à levedura íntegra não processada. Proteína e carboidrato (fibra alimentar foram os principais componentes da levedura íntegra e do autolisado. No extrato e no concentrado protéico predominaram proteína e minerais (cinzas. O autolisado e a levedura íntegra apresentaram os melhores índices de aminoácidos essenciais, seguidos pelo concentrado protéico e pelo extrato. A digestibilidade da proteína variou de 68% para a levedura íntegra a 91% para o extrato. Os índices de quociente de utilização líquida da proteína variaram de 2,1 para a levedura íntegra a 4,3 para a caseína (referência. Não houve diferença estatística no quociente de utilização líquida da proteína entre o autolisado (4,1, o extrato (3,9 e o concentrado protéico (4,2. O concentrado protéico promoveu o maior crescimento no período (21 dias, seguido do extrato e o autolisado. As células íntegras apresentaram a menor capacidade para promover crescimento em rato.The objective of this work was to promote the autolysis and the fractionation of the yeast (Saccharomyces sp. for the production of autolysate and extract, as well as phosphorylated protein concentrate, from ethanol distillery yeast. Comparative studies of centesimal composition, essential amino acid profiles and protein nutritive value were performed for the unprocessed integral cells, and for autolysate, extract and phosphorylated protein concentrate. Protein and carbohydrate (dietary fiber were the main components for the integral cells and autolysate. For the extract and the protein

  5. Cálculo das recomendações de ingestão protéica: aplicação a pré-escolar, escolar e adulto utilizando alimentos brasileiros Calculation for recommendations regarding protein intake: their application to children and adults taking Brazilian foods

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    J. Sérgio Marchini

    1994-04-01

    Full Text Available As recomendações de ingestão protéica variam conforme o teor de proteína, da quantidade de aminoácidos essenciais oferecidas e da digestibilidade da dieta. Em geral as recomendações protéicas tomam por base um alimento considerado padrão como o ovo ou o leite. No entanto, a nível populacional, ingere-se misturas de alimentos em quantidades e qualidades diferentes, que variam de região para região. A título de ilustração foram calculadas as recomendações de ingestão protéica para uma mistura de alimentos habitualmente usados pela população brasileira e consumida por três grupos etários diferentes: o pré escolar, o escolar e o adulto. São apresentadas 10 combinações de alimentos, todas tendo como principal fonte protéica o arroz e/ou o feijão. Observa-se que a recomendação de ingestão protéica do pré escolar, pode variar entre 1,15 a 1,77 g/kg por dia dependendo da mistura utilizada. Discute-se também a importância da quantidade de alimentos ingeridos e a presença de outros nutrientes. Sugere-se que ao se recomendar a ingestão protéica de uma determinada mistura de alimentos sejam considerados os seguintes fatores: teor total de nitrogênio, quantidade de aminoácidos essenciais, digestibilidade, peso total da mistura e a presença de outros nutrientes.The recommendations for protein consumption depend on the essential amino acid and total nitrogen content of a diet, and food digestibility. International recommendations are based on egg or milk proteins. However, populations eat different food mixtures. Brazilians use rice and beans as their main protein food source. This study presents different Brazilian diets, with variable amount of rice and beans. The results show that for each diet there is a different amount of protein recommended. Pre-school children, for example, must receive from 1.15 to 1.77 g/protein/day, depending on the mixture of their dietary protein intake. Besides the diet protein's quality

  6. Qualidade protéica de pão de sal contendo farinha de linhaça (Linum usitatissimum L.
    Protein quality of “french” bread containing flaxseed flour

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    J. T. S. BORGES

    2010-09-01

    Full Text Available

    As proteínas são moléculas essenciais aos organismos animais, devendo, portanto, estar presentes na alimentação em quantidades adequadas. Além do aspecto quantitativo deve-se considerar o aspecto qualitativo, isto é, seu valor nutricional, que dependerá da composição, digestibilidade, biodisponibilidade de aminoácidos essenciais, ausência de toxicidade e de fatores antinutricionais. O objetivo do presente estudo foi determinar a digestibilidade in vivo e a qualidade nutricional de proteínas de pães de sal obtidos com farinhas mistas de trigo e linhaça integral nas proporções de 90:10 e 85:15. Os melhores resultados para ganho de peso, coefi ciente de efi cácia alimentar, coefi ciente de efi cácia protéica e razão protéica líquida foram obtidos quando maior teor de farinha de trigo foi substituído pela farinha integral de linhaça na formulação dos pães. A lisina foi o primeiro aminoácido limitante em todos os produtos elaborados. A substituição parcial da farinha de trigo pela farinha de linhaça não melhorou a qualidade protéica dos pães estudados.

  7. Morphological and quantitative study of the myenteric plexus of the ascending colon of rats subjected to proteic desnutrition Estudo morfológico e quantitativo do plexo mientérico do colo ascendente de ratos submetidos a desnutrição protéica

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    Débora de Mello Gonçales Sant'Ana

    1997-01-01

    Full Text Available We carried out this study with the purpose of contributing on the effects of the proteic desnutrition on the morphological aspects and quantitative analysis of the neurons in the myenteric plexus of the ascending colon of adult Rattus norvegicus. Twenty adult rats were divided into two groups; in one of them, we offered a normal ration with proteic level of 22% (control group and in the other, a ration with a proteic level of 8% (experiment group during 120 days. We did the whole-mount preparations for the ascending colon and stained them with the Giemsa technique and the histochemical technique of NADH-diaphorase. The rats with proteic desnutrition showed a body weight, on average, to be 35.1% less than those of the control group, and the colon was on average, 26.8% shorter and 6.7% narrower. Thus, it was to be expected that the colon of animals with proteic desnutrition had a neuronal density 31.62% greater than the rats of the control group. Nevertheless, the difference with the Giemsa technique was on average 18.4%, demonstrating a mean neuronal loss of 13.25%.Realizamos este estudo com o objetivo de analisar os efeitos da desnutrição protéica sobre os aspectos morfológicos e quantitativos dos neurônios do plexo mientérico do colo ascendente de Rattus norvegicus adultos. Vinte ratos adultos foram divididos em 2 grupos, sendo que para um dos grupos ofertamos ração normal, com teor protéico de 22% (controle e para o outro ração com teor protéico de 8% durante 120 dias. Elaboramos os preparados de membrana do colo ascendente e coramos pelo método de Giemsa e pela técnica histoquímica da NADH-diaforase. Os ratos com desnutrição protéica apresentaram peso corporal em média 35,1% menor que os do grupo controle, e o colo era em média 26,8% mais curto e 6,7% mais estreito. Esperava-se que o colo dos animais desnutridos possuíssem uma densidade neuronal 31,62% maior que o dos animais normonutridos; no entanto, a diferença com a

  8. Avaliação da expressão gênica da proteína aspartil secretada 2, 5 e 9 (SAP-2, SAP-5 e SAP-9) e sua correlação com a invasão epitelial por Candida albicans em modelo experimetal de estomatite protéica in vivo

    OpenAIRE

    Priscila Lie Tobouti

    2011-01-01

    A Estomatite protética associada a Candida (EPC) acomete a mucosa bucal em contato com próteses removíveis e, clinicamente, caracteriza-se por eritema com diferentes graus de inflamação. Esta doença é considerada de etiologia multifatorial, isto é, uma associação de fatores como trauma, falta de higienização, uso contínuo da prótese, hipersensibilidade ao material usado na dentadura, diabetes, terapia imunossupressora e infecção por fungo, como diferentes espécies de Candida. Os principais f...

  9. Incorporação de uréia encapsulada em suplementos protéicos fornecidos para novilhos alimentados com feno de baixa qualidade Incorporation of coated urea in protein supplements supplied to steers fed with low quality hay

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    Eduardo Bohrer de Azevedo

    2008-08-01

    Full Text Available Foi realizado um experimento com o objetivo de verificar o efeito da suplementação com uréia encapsulada ou normal sobre a utilização de volumoso de baixa qualidade em novilhos. Os tratamentos foram: Feno + sal mineralizado; Feno + suplemento protéico com uréia comum; Feno + suplemento protéico com uréia encapsulada fórmula 1; Feno + suplemento protéico com uréia encapsulada fórmula 2. O volumoso utilizado foi feno de Tifton (Cynodon dactylon L. de baixa qualidade (PB: 4,62%, FDN: 83,46%. Foram realizadas medidas de digestibilidade, consumo e cinética digestiva. A relação entre o consumo de proteína degradável no rúmen e o consumo de matéria orgânica digestível foi maior (P0,05, ao comparar suplementados ou não suplementados com PDR, e da mesma forma ao comparar fontes de nitrogênio não-protéico. A suplementação de proteína degradável no rúmen não foi efetiva em alterar os parâmetros estudados, assim como as fontes de uréia encapsulada mostraram respostas semelhantes à uréia comum.A trial was accomplished with the objective to verify the effect of the coated or common urea supplementation on the utilization of low quality hay in steers. The treatments were: hay + mineral supplement; hay + protein supplement with common urea; hay + protein supplement with coated urea formula 1; hay + protein supplement with coated urea formula 2. Tifton (Cynodon dactylon L. hay of low quality (CP: 4.62%, NDF: 83.46% was used. The measures were: digestibility, intake and digestive kinetics. The relation between degradable intake protein and digestible organic mater intake was highest (P0.05 when compared the supplemented or not supplemented diets with DIP and in the same way, when compared the non-protein nitrogen sources. The ruminal degradable protein supplementation was not effective for modifying the studied parameters, as well as, the coated urea sources showed to be similar to the common urea.

  10. Desempenho de Novilhos Santa Gertrudis Confinados Submetidos a Dietas com Diferentes Fontes Protéicas e Silagem de Milho, com ou sem Inoculante Animal Performance of Feedlot Santa Gertrudis Steers Fed Diets with Different Protein Sources and Corn Silage, with or without Inoculant

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    Alfredo Acosta Backes

    2001-12-01

    Full Text Available O presente trabalho foi conduzido com o objetivo de avaliar o efeito de uma dieta alimentar contendo fontes protéicas de diferentes degradabilidades ruminais e silagem de milho preparada com ou sem inoculante PIONEER 1174, no desempenho animal. Foi conduzido um experimento fatorial, em que as fontes protéicas (farinha de carne e osso, uréia e uma mistura de ambas [50:50] foram combinadas com dois tipos de silagens, formando os seis tratamentos experimentais. Foram utilizados 36 novilhos Santa Gertrudis, com 3,5 anos de idade, distribuídos ao acaso nos tratamentos. Diferenças significativas não foram encontradas entre tratamentos para consumo de matéria seca e as interações entre fontes protéicas ou tipos de silagens também não foram significativas. Para o ganho médio diário de peso não houve diferença significativa entre tratamentos, mas a interação entre fontes protéicas mostrou-se significativa. Os animais alimentados com dietas contendo farinha de carne e ossos apresentaram ganhos significativos em relação aos alimentados com uréia. Contrastes entre tipos de silagens não mostraram diferença.The present work was carried out to evaluate the effect of diets with different protein sources of ruminal degradability and corn silage prepared with or without the inoculant PIONEER 1174 on animal performance. A factorial experiment was conducted, where the protein sources (meat and bone meal, urea and a mixture of both [50:50] were combined with two types of silage, composing six treatments. Thirty six 3.5 years old Santa Gertrudis steers were randomly assigned to the treatments. There were no difference between treatments for dry matter intake, not even when the interactions were made among protein sources or types of silage. For average daily gain there were no significant differences among treatments, but the interactions among protein sources were significant. Animals fed diets with meat and bone meal showed higher weight

  11. Câbles électriques - Calcul du courant admissible - Partie 2: Résistance thermique - Section 2: Méthode de calcul des coefficients de réduction de l'intensité de courant admissible pour des groupes de câbles posés à l'air libre et protégés du rayonnement solaire direct

    CERN Document Server

    1995-01-01

    Câbles électriques - Calcul du courant admissible - Partie 2: Résistance thermique - Section 2: Méthode de calcul des coefficients de réduction de l'intensité de courant admissible pour des groupes de câbles posés à l'air libre et protégés du rayonnement solaire direct

  12. Caracterização e avaliação sensorial de barra de cereais funcional de alto teor protéico e vitamínico Characterization and sensorial evaluation of functional cereal bar

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    Daniela G.C. Freitas

    2006-06-01

    Full Text Available Barras de cereais foram introduzidas no mercado há cerca de uma década como alternativa "saudável" de confeito. Concomitantemente, alimentos como a soja, ricos em vitaminas e minerais e com propriedades antioxidantes vêm recebendo grande aplicação em produtos de alegação funcional, pois previnem contra doenças cardiovasculares e crônico-degenerativas. Considerando o crescimento do segmento de barra de cereais no setor de alimentos, desenvolveu-se uma formulação de barra de cereais de alto teor protéico e vitamínico, à base de proteína de soja texturizada, gérmen de trigo e aveia, enriquecida de ácido ascórbico e acetato de -tocoferol. As matérias-primas e a formulação final da barra de cereais foram submetidas a análises para caracterização e três formulações, variando o teor de ácido ascórbico, foram avaliadas quanto à preferência sensorial e intensidade ideal de doçura e acidez. A proteína de soja texturizada utilizada apresentou elevados teores dos oligossacarídeos rafinose e estaquiose (1,92 g/100 g e 4,66 g/100 g e de isoflavonas totais (283,49 mg/100 g se comparados ao grão, farinha integral e isolado protéico. A formulação final da barra de cereais apresentou 15,31% de proteína e elevado teor de vitamina E (118,0 mg/100 g e minerais como fósforo, ferro e manganês. A formulação adicionada de 1,1 g/100 g de ácido ascórbico obteve maior preferência sensorial diferindo das demais amostras (pCereal bars were introduced over a decade ago as a "healthy" alternative confectionery. Concomitantly, foods as soybean, with high vitamin and mineral levels and antioxidants properties come receiving great application in functional products, because they can prevent against chronic-degenerative illnesses of the heart. Considering the growth of the segment of cereal bars in the food sector, it was developed a formulation with high protein and vitamin levels cereal bar based on textured soy protein, wheat germ

  13. Diferentes fontes protéicas para a alimentação do jundiá (Rhamdia quelen Different protein sources for jundiá (Rhamdia quelen feeding

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    Rafael Lazzari

    2006-02-01

    Full Text Available O objetivo deste trabalho foi verificar o crescimento e a composição de filés de juvenis de jundiá (Rhamdia quelen alimentados com diferentes fontes protéicas, durante 60 dias. Utilizaram-se 540 peixes (peso médio inicial=15,00±0,62g, comprimento total inicial=11,98±0,35cm distribuídos ao acaso em 18 caixas de 280L (30 peixes/caixa em sistema de recirculação de água. Testaram-se seis tratamentos (com três repetições: CL (farinha de carne e ossos + levedura, SL (farelo de soja + levedura, S (somente farelo de soja, CS (farinha de carne e ossos + farelo de soja, PL (farinha de peixe + levedura e PS (farinha de peixe + farelo de soja. Foram estimados parâmetros de desempenho (peso, comprimento total, fator de condição, taxa de crescimento específico, taxa de eficiência protéica aos 30 e 60 dias, composição centesimal e taxas de deposição de proteína e gordura nos filés ao final do experimento. Os parâmetros peso, comprimento total e taxa de crescimento específico foram superiores nos tratamentos compostos pela combinação das farinhas de origem animal (carne e ossos e peixe com farelo de soja. A quantidade de gordura e proteína depositada no filé dos peixes também foi superior nestes tratamentos (CS e PS. Os rendimentos de carcaça e filé não diferiram entre os tratamentos. A combinação das farinhas de carne e ossos e de peixes com farelo de soja possibilita bom crescimento e maior deposição de proteína e gordura nos filés de juvenis de jundiá.This study aimed at verifing the growth and fillet composition of jundiá juveniles (Rhamdia quelen fed with different protein sources, for 60 days. It was used 540 fish (initial weight=15.00±0.62g, initial length=11.98±0.35cm at random distributed in 18 tanks of 280L (30 fish/tank in a water re-use system. 6 treatments were tested (with 3 repetitions: CL (meat and bone meal + yeast, SL (soybean meal + yeast, S (only soybean meal, CS (meat and bone meal + soybean

  14. Membranas híbridas basadas en estireno-metacrilato-sílice y ácido fosfowolfrámico obtenidas por sol-gel para pilas de combustible de intercambio protónico (PEMFC

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    Mosa, J.

    2007-10-01

    Full Text Available Contrary to internal combustion engines, proton-exchange membrane fuel cells (PEMFC used in transportation operate with zero emissions of environmental pollutants. The increase of the operation temperature in PEMFC above 100°C is a great concern for the application of this type of cells in electric vehicles. Hybrid organic-inorganic membranes with nanosized interfaces can combine the main properties of their components to meet this objective. Styrene-methacrylate-silica membranes doped with phosphotungstic acid (PWA were prepared through acid catalyzed sol-gel process and free-radical copolymerization. Additionally, sulfonation processes of aromatic rings to produce attached SO3H groups were applied to increase the proton conductivity. The effect of sulfonation degree and PWA doping on the membrane properties such as chemical and thermal stability, water uptake, ion exchange capacity, and proton conductivity were investigated. The measurement of conductivity shows a general increase with rising temperatures and with the increasing of SO3H groups density, reaching a maximum value of 3.2 10-3 S/cm at 130ºC and 100%HR.

    Comparadas con los motores de combustión interna, las pilas de combustible de intercambio de protones (PEMFC son capaces de operar sin emisiones de agentes contaminantes. El aumento de la temperatura de operación de la pila de combustible por encima de 100ºC es uno de los grandes objetivos en este campo ya que facilitaría el desarrollo comercial de los vehículos eléctricos impulsados por pilas de combustible. Las membranas híbridas orgánico-inorgánicas nanoestructuradas combinan las propiedades necesarias para este tipo de aplicación. Se obtuvieron membranas híbridas dopadas con ácido fosfowolfrámico (PWA por copolimerización radicálica a partir de alquilalcóxidos y monómeros de estireno y metacrilato, y por reacción sol-gel vía catálisis ácida. La conductividad protónica se logra realizando un proceso

  15. Calibração da técnica de moiré aplicada a perfilometria de protótipos mecânicos Calibration method applied to a moiré technique in experiments of mechanical archetypes

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    Túlio Seabra Gomes

    2009-04-01

    Full Text Available A perfilometria é uma técnica amplamente utilizada na construção de mapas e gráficos de relevos aplicados a uma vasta área do conhecimento. Frente às necessidades de suprir a demanda da indústria e da área de desenvolvimento de protótipos quanto a técnicas não invasivas na investigação da superfície de materiais em geral, o presente trabalho buscou propor uma metodologia de calibração aplicada à técnica de moiré para investigações perfilométricas em protótipos mecânicos buscando baixos custos e flexibilidade. A técnica de moiré consiste na comparação de dois retículos periódicos, onde um segue o comportamento da superfície do objeto (retículo modelo - Rm e outro não está deformado, seguindo o comportamento de um plano de referência, (retículo de referência - Rr. A luz que passa entre os retículos se sobrepõe formando padrões de moiré ou franjas de moiré que se comportam como ondas senoidais. Nos ensaios de calibração, usou-se um cone de relevo conhecido para a determinação de uma constante de correção de mapas gerados digitalmente. Os ensaios realizados aplicaram a metodologia para a determinação do perfil de um mouse de computador e para a determinação da deformação de uma chapa metálica. A metodologia proposta para a calibração da técnica de moiré mostrouse capaz de realizar os mapeamentos, com resolução máxima na ordem de centésimos de milímetros, podendo então ser usada em aplicações com níveis de precisão inferiores a essa ordem.Profilometry is a measurement technique widely used in map and relief graphic construction, being applied to vast areas of knowledge. The aim of the present investigation was to introduce a calibration method applied to a moiré technique in experiments of mechanical archetypes, with low cost and flexibility, due to the need for supplying demands from both industry and mechanical archetype development for non-invasive techniques. The moir

  16. Suplementação protéica energética no desempenho de novilhas em pastejo durante a fase de terminação Energetic protein supplementation on heifers performance in grazing during the finish phase

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    Matheus Henrique Moretti

    2011-06-01

    Full Text Available Para estudar o efeito da suplementação da dieta sobre o desempenho de novilhas na fase de terminação mantidas em pastagem de capim-marandu sob lotação intermitente, durante a estação chuvosa, foram utilizadas 20 novilhas cruzadas (¼ Nelore, ¼ Santa Gertrudes, ½ Braunvieh com peso corporal médio de 300 kg e 22 meses de idade. Os tratamentos constituíram-se de dois tipos de suplementação: sal mineral (SM ad libtum e suplemento protéico energético (SPE fornecido a 0,3% do peso corporal (PC por dia com avaliações realizadas a cada 28 dias, no período entre dezembro de 2006 e março de 2007 Foram avaliados o desempenho animal e as características de carcaça medidas por ultrassom. Observou-se o efeito (P=0,057 da suplementação sobre o ganho de peso dos animais suplementados, 0,700 kg/dia e 0,587 kg/dia dos animais que receberam sal-mineral. Houve efeito da suplementação na área de olho de lombo e profundidade do músculo Gluteus medius. As demais características analisadas pela técnica de ultrassom não diferiram entre si em função da suplementação. A suplementação protéica energética da dieta permite ganhos adicionais, o que reflete em aumento da deposição de músculo de novilhas mantidas em pastagem de capim-marandu durante o período das águas.This study was conducted to evaluate the effect of the diet supplementation on the heifers performance in finishing phase, maintained in a marandu grass pasture managed in intermittent grazing system during the rainy season. Twenty crossbreed (¼ Nelore, ¼ Santa Gertrudes, ½ Braunvieh heifers with average initial body weight of 300 kg and 22 months old were used. The treatments consisted of two types of supplements: mineral (SM ad libtum and energy protein supplementation (PES provided 0.3% of the body weight daily. The evaluated variables were: animal performance and carcass traits measured by ultrasound each 28 days. Supplementation affected daily weight gain (P=0

  17. Desempenho de novilhos suplementados com sal mineral protéico e energético em pastagem no período da seca = Performance of Steers Supplemented in Pasture with Protein-energy Mineral Salt in dry season

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    Euclides Reuter de Oliveira

    2006-07-01

    Full Text Available O trabalho foi realizado objetivando avaliar os efeitos energético e protéico em uplementos múltiplos sobre o ensaio de produção, concentração do N uréico no plasma e parâmetros econômicos de bovinos em fase de recria, manejados em pastagens, durante a época da seca. Foram utilizados 32 novilhos de corte divididos em 4 grupos de 8 animais e analisados por um delineamento de blocos ao acaso a 5% de significância. Cada grupo recebeu um tratamento com pastagem e acrescido de sal mineralizado + uréia + milho; sal mineralizado + uréia + farelo de soja; sal mineralizado + uréia + milho + farelo de soja. Não observou diferença (P>0,05 das suplementações no ganho de peso e o peso médio metabólico, o que pode ser justificado pela qualidade da pastagem aliada à disponibilidade de MS/ha e para a variável N-uréico no sangue indicou o maior teor protéico, oriundo dos 50,0% de participação do farelo de soja na composição do tratamento. A suplementação para animais em pastejo com misturas de natureza múltipla e de baixo consumo proporcionou ganhos de pesoapreciáveis, e sua aplicação depende do preço dos insumos e o da arroba de carne.The research was carried out to evaluate the protein-energy effect of multiple supplements on animal production, N urea in plasmatic quantities and economic parameter of growing beef cattle in pasture during dry season. Thirty-two castrated beef cattle steers were divided into four groups of eight animals. They were analyzedaccording to a randomized block design at 5% of significance. Each group received one treatment to pasture with addition of mineral salt + urea + corn; mineral salt + urea + soybean meal; mineral salt + urea + corn + soybean meal. There was no difference (P>0.05 betweensupplements for daily LWG and for average metabolic weight. This can be justified by forage quality combined with availability of DM/ha. As for the variable N-urea in the blood, results indicated higher protein

  18. Effects of the condylar process fracture on facial symmetry in rats submitted to protein undernutrition Efeitos da fratura do processo condilar na simetria facial em ratos submetidos à desnutrição protéica

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    Lucimar Rodrigues

    2011-04-01

    Full Text Available PURPOSE: To investigate the facial symmetry of rats submitted to experimental mandibular condyle fracture and with protein undernutrition (8% of protein by means of cephalometric measurements. METHODS: Forty-five adult Wistar rats were distributed in three groups: fracture group, submitted to condylar fracture with no changes in diet; undernourished fracture group, submitted to hypoproteic diet and condylar fracture; undernourished group, kept until the end of experiment, without condylar fracture. Displaced fractures of the right condyle were induced under general anesthesia. The specimens were submitted to axial radiographic incidence, and cephalometric mensurations were made using a computer system. The values obtained were subjected to statistical analyses among the groups and between the sides in each group. RESULTS: There was significative decrease of the values of serum proteins and albumin in the undernourished fracture group. There was deviation of the median line of the mandible relative to the median line of the maxilla, significative to undernutrition fracture group, as well as asymmetry of the maxilla and mandible, in special in the final period of experiment. CONCLUSION: The mandibular condyle fracture in rats with proteic undernutrition induced an asymmetry of the mandible, also leading to consequences in the maxilla.OBJETIVO: Investigar a simetria facial de ratos submetidos à fratura experimental de côndilo mandibular e com desnutrição protéica (8% de proteína por meio de mensurações cefalométricas. MÉTODOS: 45 ratos Wistar adultos foram distribuídos em três grupos: grupo fraturado, submetido a fratura condilar sem alteração na dieta; grupo fraturado desnutrido, submetido a dieta hipoprotéica e fratura condilar; grupo desnutrido, mantido até o final do experimento, sem fratura condilar. Fraturas com desvio foram feitas no côndilo direito com anestesia geral. Os espécimes foram submetidos à incidência radiogr

  19. Isolado protéico de semente de goiaba (Psidium guajava: caracterização de propriedades funcionais Protein isolate of guava seed (Psidium guajava: functional properties of characterization

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    Gustavo Guadagnucci Fontanari

    2007-08-01

    Full Text Available Isolados protéicos (IPs foram obtidos a partir da farinha da semente de goiaba (Psidium guajava por extração a diferentes pHs seguido de precipitação isoelétrica (pI 4,5. As condições para o preparo do IP foram definidas a partir da curva de solubilidade, em pH 10,0 e 11,5, na ausência de NaCl e em temperatura de 25 ± 3 ºC. Essas condições levaram a um rendimento na extração de 45,19 e 66,23%, e teor protéico de 96,4 e 93,5% para IP 10,0 e 11,5, respectivamente. Capacidade de absorção de água e óleo de 1,05 ± 0,07 e 2,30 ± 0,01 mL.g-1 proteína e 1,65 ± 0,07 e 1,70 ± 0,07 mL.g-1 proteína foram obtidos, respectivamente, para IP 10,0 e IP 11,5. Não foram observadas diferenças entre os IPs quanto à atividade e estabilidade de emulsão, atingindo valores similares e superiores ao IP de outras sementes. Formação de gel, em pH neutro e ausência de sal, exigiram concentrações de 8 e 10% para IP 10,0 e IP 11,5, respectivamente. Maior capacidade de formação de espuma foi obtida para IP 10,0 em pH neutro e alcalino, apresentando valores de até 90% do volume inicial, por outro lado, para estabilidade de espuma, ambos IPs apresentaram boas propriedades até duas horas após a formação da espuma.Protein isolates (PIs were obtained from the guava seed flour (Psidium guajava by extracting different pHs followed by isoelectric precipitation (pH 4.5. The conditions for preparation of the PIs were defined from the solubility curve at pH 10.0 and 11.5, in the absence of NaCl and a temperature of 25 ± 3 ºC. These conditions obtained protein isolated (PI with extraction yield of 45.19 and 66.23%, with protein content of 96.4 and 93.5% for PI 10.0 and 11.5, respectively. The water and oil absorption capacity of 1.05 ± 0.07 and 2.30 ± 0.01 mL.g-1 protein and 1.65 ± 0.07 and 1.70 ± 0.07 mL.g-1 protein, respectively; were obtained for PI 10.0 and PI 11.5. Differences among the PIs regarding the emulsion activity and stability

  20. Regulação da ingestão protéica na tilápia do Nilo, Oreochromis niloticus Hability of protein intake regulation in Nile Tilapia, Oreochromis niloticus

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    Elyara Maria Pereira-da-Silva

    2004-12-01

    Full Text Available Estudou-se a habilidade de juvenis de tilápia do Nilo, Oreochromis niloticus, em regular a ingestão protéica. Mil exemplares com peso e comprimento de 13,93g ± 0,87g e 8,8cm ± 0,47cm, respectivamente, revertidos sexualmente, foram distríbuídos em oito grupos de 125 peixes, em aquários de 1.000 L com renovação contínua de água e dotados de dois alimentadores de demanda. Duas rações isoenergéticas (2.800 kcal/kg contendo, respectivamente, 40% e 15% PB foram oferecidas diariamente, ad libitum, em combinações que caracterizaram quatro tratamentos: A: ração de 15% PB de um lado e de 40% PB do outro do aquário; B: idêntico ao tratamento A com inversão dos lados; C: ração de 15% PB fornecida em dois alimentadores; e D: ração de 40% PB fornecida em dois alimentadores. O delineamento em quadrado latino permitiu que os peixes fossem submetidos a cada tratamento durante quatro fases de 15 dias (I, II, III e IV. Entre as fases, houve intervalo de uma semana, quando os peixes receberam dieta contendo 30% PB nos dois alimentadores. O consumo alimentar dos peixes, registrado diariamente, não diferiu significativamente entre os tratamentos A e B. Porém, quando foram apresentadas simultaneamente as dietas contendo mesmo teor de PB (Tratamentos C e D, observou-se consumo significativamente maior da ração contendo 15% de PB (Tratamento C. O ajuste em porcentagem da proteína ingerida pelos peixes revelou média de 24% de PB. Os resultados confirmaram a hipótese de que a tilápia do Nilo apresenta habilidade em regular a ingestão dietética protéica por meio de livre escolha.The free-choice feed and regulation of protein intake were investigated in juveniles reverted males of tilápia do Nilo, Oreochromis niloticus. During the experiment 1000 fish (reverted males with similar weigth and length were maintained in eight aquariuns supplied with recirculated water (groups of 125 fish each aquarium. Fish were fed by demand