Mathematical Model Of Dispersion Of Pollutants In The Magdalena River Using Tracer; Modelo Matematico de Dispersion de Contaminantes en el Rio Magdalena utilizando trazadores

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Fiesco V, Yolanda; Ruiz V, Fernando A

1992-07-01

The constant increase of the indexes of contamination of the Magdalena river either caused by the riverside populations or by flowing as the Bogota, Cauca, San Jorge rivers and other forecast hopeless consequences at National level as much in the social field as in the economic one. We consider that the results obtained in this study will contribute thoroughly in the knowledge of the river and later, they will be taken as a fundamental tool in studies that the National Government will undertake, if it wants to save the most important fluvial system in the country from contamination. This project of technical cooperation undersigned between the IAEA and the IAN on behalf of Colombian Government, refers to the use of isotopic techniques to determine hydraulic parameters of the Magdalena river. The scheme of the river included in this study covers the outlet of Bogota River in Girardot, until its outlet in Barranquilla.

Simulating Spatial Variability of Fluvial Sediment Fluxes Within the Magdalena Drainage Basin, Colombia.

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Kettner, A. J.; Syvitski, J. P.; Restrepo, J. D.

2008-12-01

This study explores the application of an empirical sediment flux model BQART, to simulate long-term sediment fluxes of major tributaries of a river system based on a limited number of input parameters. We validate model results against data of the 1612 km long Magdalena River, Colombia, South America, which is well monitored. The Magdalena River, draining a hinterland area of 257,438 km2, of which the majority lies in the Andes before reaching the Atlantic coast, is known for its high sediment yield, 560 t kg- 2 yr-1; higher than nearby South American rivers like the Amazon or the Orinoco River. Sediment fluxes of 32 tributary basins of the Magdalena River were simulated based on the following controlling factors: geomorphic influences (tributary-basin area and relief) derived from high-resolution Shuttle Radar Topography Mission data, tributary basin-integrated lithology based on GIS analysis of lithology data, 30year temperature data, and observed monthly mean discharge data records (varying in record length of 15 to 60 years). Preliminary results indicate that the simulated sediment flux of all 32 tributaries matches the observational record, given the observational error and the annual variability. These simulations did not take human influences into account yet, which often increases sediment fluxes by accelerating erosion, especially in steep mountainous area similar to the Magdalena. Simulations indicate that, with relatively few input parameters, mostly derived from remotely-sensed data or existing compiled GIS datasets, it is possible to predict: which tributaries in an arbitrary river drainage produce relatively high contributions to sediment yields, and where in the drainage basin you might expect conveyance loss.

Acumulación capitalista y nueva espacialidad en el Magdalena Medio

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Christian A. Moreno Sarmiento

2012-01-01

Full Text Available Este trabajo se propone identificar y presentar las tendencias del desarrollo capitalista en el Magdalena Medio colombiano desde el siglo XX, así como las relaciones con las dinámicas de acumulación nacional y global. Sostenemos que la producción social del espacio denominado Magdalena Medio ha seguido la ruta de procesos de acumulación por desposesión, ya sea en sus formas de vinculación a la lógica de producción capitalista o en las transformaciones de las formas de producción capitalistas allí donde ya existen; procesos que han estado atravesados por reconfiguraciones en las relaciones de poder de clase, del régimen político y de la dinámica del conflicto social y armado.

Myc-nick: a cytoplasmic cleavage product of Myc that promotes alpha-tubulin acetylation and cell differentiation.

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Conacci-Sorrell, Maralice; Ngouenet, Celine; Eisenman, Robert N

2010-08-06

The Myc oncoprotein family comprises transcription factors that control multiple cellular functions and are widely involved in oncogenesis. Here we report the identification of Myc-nick, a cytoplasmic form of Myc generated by calpain-dependent proteolysis at lysine 298 of full-length Myc. Myc-nick retains conserved Myc box regions but lacks nuclear localization signals and the bHLHZ domain essential for heterodimerization with Max and DNA binding. Myc-nick induces alpha-tubulin acetylation and altered cell morphology by recruiting histone acetyltransferase GCN5 to microtubules. During muscle differentiation, while the levels of full-length Myc diminish, Myc-nick and acetylated alpha-tubulin levels are increased. Ectopic expression of Myc-nick accelerates myoblast fusion, triggers the expression of myogenic markers, and permits Myc-deficient fibroblasts to transdifferentiate in response to MyoD. We propose that the cleavage of Myc by calpain abrogates the transcriptional inhibition of differentiation by full-length Myc and generates Myc-nick, a driver of cytoplasmic reorganization and differentiation. Copyright 2010 Elsevier Inc. All rights reserved.

La participación ciudadana en la construcción del espacio público de la parroquia la Magdalena. Período 2009 - 2014

OpenAIRE

Santacruz Cevallos, Mónica Patricia

2015-01-01

The aim of this study was to determine the levels of public participation in the construction of public space in the parish of La Magdalena, and the impact they have this space in local development thereof, the theoretical framework developed for understanding processes existing citizen participation in the parish of La Magdalena and its relation to the construction of public space in it. In chapter two the development and territorial occupation of the parish of La Magdalena and cultural e...

Estrategias de aprendizaje y rendimiento académico en una Institución Educativa de Magdalena 2014

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Viaña Frit, Carmen Cecilia

2015-01-01

La investigación titulada, “Uso de las estrategias de aprendizaje y el rendimiento académico de los estudiantes del VII ciclo del nivel secundaria de la I.E. Francisco Bolognesi de Magdalena 2014”, cuyo objetivo fue determinar la relación que existe entre el uso de las estrategias de aprendizaje y el rendimiento académico de los estudiantes del VII ciclo del nivel secundaria de la I.E. Francisco Bolognesi de Magdalena 2014, como un aporte al análisis, descripción y explicación ...

Divalent metal ion differentially regulates the sequential nicking reactions of the GIY-YIG homing endonuclease I-BmoI.

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Benjamin P Kleinstiver

Full Text Available Homing endonucleases are site-specific DNA endonucleases that function as mobile genetic elements by introducing double-strand breaks or nicks at defined locations. Of the major families of homing endonucleases, the modular GIY-YIG endonucleases are least understood in terms of mechanism. The GIY-YIG homing endonuclease I-BmoI generates a double-strand break by sequential nicking reactions during which the single active site of the GIY-YIG nuclease domain must undergo a substantial reorganization. Here, we show that divalent metal ion plays a significant role in regulating the two independent nicking reactions by I-BmoI. Rate constant determination for each nicking reaction revealed that limiting divalent metal ion has a greater impact on the second strand than the first strand nicking reaction. We also show that substrate mutations within the I-BmoI cleavage site can modulate the first strand nicking reaction over a 314-fold range. Additionally, in-gel DNA footprinting with mutant substrates and modeling of an I-BmoI-substrate complex suggest that amino acid contacts to a critical GC-2 base pair are required to induce a bottom-strand distortion that likely directs conformational changes for reaction progress. Collectively, our data implies mechanistic roles for divalent metal ion and substrate bases, suggesting that divalent metal ion facilitates the re-positioning of the GIY-YIG nuclease domain between sequential nicking reactions.

Variabilidad de la abundancia de zooplancton en Bahía Magdalena Baja California Sur, México (1997-2001 Zooplankton abundance variability in Magdalena Bay, Baja California Sur, Mexico (1997-2001

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Sergio Hernández-Trujillo

2010-01-01

Full Text Available Se analizaron muestras de zooplancton de 16 campañas oceanógraficas, efectuadas en Bahía Magdalena, Baja California Sur, México, entre agosto de 1997 y marzo de 2001. Se identificó un total de 26 grupos taxonómicos, de los cuales los más abundantes y frecuentes fueron copépodos y quetognatos; en 2000-2001 se observó una tendencia a disminuir entre 10 y 20 el número de grupos de zooplancton. La biomasa zooplanctónica y abundancia de copépodos disminuyeron en el periodo de estudio, en contraste con los quetognatos que tuvieron un ligero aumento. Las fluctuaciones de abundancia de zooplancton no estuvieron relacionadas con la concentración de clorofila-α, a diferencia de los máximos de abundancia de zooplancton, que estuvieron asociados a los cambios de la temperatura superficial del mar. El ciclo estacional de la abundancia del zooplancton en Bahía Magdalena, indicó que en invierno el promedió fue mayor de 65.000 ind 100 m-3 , valor que aumentó en primavera a más de 99.000 ind 100 m-3 , se mantuvo en verano alrededor de 100.000 ind 100 m-3 y en otoño descendió rápidamente a casi 40.000 ind 100 m-3.Zooplankton were studied from 16 oceanographic surveys carried out in Magdalena Bay, Baja California Sur, Mexico, between August 1997 and March 2001. Twenty-six taxonomic groups were identified, the most abundant and frequent of which were copepods and chaetognaths. In 2000-2001, the number of zooplankton groups tended to decrease by 10 to 20. Both zooplankton biomass and copepod abundance declined, unlike chaetognaths, which increased slightly. Fluctuations in zooplankton abundance were independent of the chlorophyll-a concentration, whereas the maximum zooplankton abundances were associated with changes in the sea surface temperature. The seasonal zooplankton abundance cycle in Magdalena Bay indicated that, in winter, the averaged was than 65,000 ind 100 m-3 , a value that increased to more than 99,000 ind 100 m-3 in spring

Community structure of zooplankton in the main entrance of Bahía Magdalena, México during 1996

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Jaime Gómez-Gutiérrez

2001-06-01

Full Text Available The zooplankton community structure, including copepods, euphausiids, chaetognaths, and decapod larvae, was monitored during six circadian cycles using Bongo net (500 *m mesh net samples from Bahía Magdalena, on the southwest coast of Baja California, México. Samples were obtained during three oceanographic surveys (March, July, and December 1996 to describe the changes in the zooplankton community structure throughout the main mouth of Bahía Magdalena. The zooplankton community structure showed strong changes with a close relation to environmental conditions. During March, a well-mixed water column with low temperature and salinity indicated an influence of the California Current water and local upwelling processes. During July, temperature increased a nd a wide salinity range was recorded. The stratification of the water column was intense during summer, enhancing the thermocline. The highest temperatures and salinity were recorded in December, related to the presence of the Costa Rica Coastal Current (CRCC. The thermocline deepened as water temperature increased. A typical temperate community structure with low specific richness dominated by Calanus pacificus, Nyctiphanes simplex, and Acartia clausi and high zooplankton biomass (average 9.3 and 5.5 ml 1000 m-3respectively during March and July shifted to a more complex tropical community structure with a low zooplankton biomass in December (average 0.37 ml 1000 m-3. The mouth of Bahía Magdalena has a vigorous exchange of water caused by tidal currents. The zooplankton community structure was not significantly different between the central part of Bahía Magdalena and the continental shelf outside the bay for all months. The results suggest a more dynamic inside-outside interaction of zooplankton assemblages than first thought.La estructura de la comunidad de zooplancton, incluyendo copépodos, eufáusidos, quetognatos y larvas de decápodos, fue monitoriada por seis ciclos circadianos

Las 'fiebres del Magdalena': medicina y sociedad en la construcción de una noción médica colombiana, 1859-1886 'Magdalena fevers': medicine and society in the construction of a Colombian medical notion, 1859-1886

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Claudia Mónica García

2007-03-01

Full Text Available Explora los fundamentos teóricos, sociales e ideológicos del surgimiento y consolidación de la noción médica colombiana 'fiebres del Magdalena'. Muestra cómo el naciente cuerpo médico colombiano elaboró unas nociones peculiares sobre las fiebres articulando las teorías médicas europeas con la valoración negativa sobre el clima cálido. Explica la forma en que las políticas librecambistas de mediados de siglo así como el impacto económico e ideológico del boom agro exportador del tabaco y el añil determinaron el interés de los médicos por las epidemias ocurridas en los centros productores de estos bienes y, por tanto, el surgimiento de la noción 'fiebres del Magdalena'. Muestra cómo los médicos establecieron una asociación causal entre el proceso productivo de dichos artículos y las fiebres.In this article, I explore the theoretical, social and ideological bases of the emergence and consolidation of the Colombian medical notion of the 'Magdalena fevers'. Firstly I show how, in the late 1850s, the emerging Colombian body of medical doctors elaborated peculiar notions on fevers by articulating the European medical theories (i.e. the miasmatic theory and the climatic determination of diseases with the negative valuation of the hot climate. Secondly, I explain how free trade policies in the mid-1800s, and the economic and ideological impacts of the agricultural export of tobacco and indigo determined doctors' interest in the epidemics occurring in the production centers and also, therefore, the emergence of the notion of 'Magdalena fevers'. I also show how doctors established a causal association between the productive process of those goods and the fevers.

Un río difícil. El Magdalena: historia ambiental, navegabilidad y desarrollo

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Germán Márquez Calle

2016-01-01

Full Text Available El río Magdalena fue por siglos la principal y casi obligada vía de comunicación desde el interior de Colombia con el Caribe y el mundo, a pesar de que su navegación siempre fue difícil como se muestra en este artículo, basado en testimonios de viaje y otras fuentes. Las dificultades, atribuibles a causas naturales como lo empinado y erosionable de la cuenca, que propician formación de bancos de arena y mucho torrencial, se agravan luego por la deforestación. La navegación colapsará finalmente, por esta y otras causas, en la década de 1960. Pero ya desde el siglo XVIII se exploraban alternativas de transporte que se encontrarán en la salida al Pacífico, mediante ferrocarriles y carreteras empezadas terminando el siglo XIX. Las nuevas vías, y la apertura del Canal de Panamá en 1914, impulsarán el desarrollo de Cali, Buenaventura y del occidente del país, en detrimento del Caribe y especialmente de Barranquilla que, a través del Magdalena, habían sido eje del comercio exterior colombiano. Evidencia científica explica las dificultades naturales; luego, un panorama de la deforestación explica su agravamiento. Actualmente hay proyectos ambiciosos para devolver navegabilidad al río, en un proceso riesgoso donde parece importante recordar que el Magdalena siempre fue un río difícil.

Book Review: Lubańska Magdalena, Muslims and Christians in the Bulgarian Rhodopes. Studies on Religious (AntiSyncretism

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Karolina Bielenin-Lenczowska

2015-12-01

Full Text Available Book Review: Lubańska Magdalena, Muslims and Christians in the Bulgarian Rhodopes. Studies on Religious (AntiSyncretism "Muslims and Christians in the Bulgarian Rhodopes. Studies on Religious (AntiSyncretism", a book by Magdalena Lubańska is a summary of her research carried out for many years among the Christian Orthodox and Muslim Pomaks in Rhodope mountains of Bulgaria. There Lubańska conducted in-depth interviews and carried out ethnographic observation about the knowledge regarding neighbours of different religion, their beliefs and religious practices.   Recenzja książki: Lubańska Magdalena, Muslims and Christians in the Bulgarian Rhodopes. Studies on Religious (AntiSyncretism Książka Magdaleny Lubańskiej jest podsumowaniem jej badań prowadzonych od wielu lat wśród prawosławnych oraz muzułmanów w Rodopach, w Bułgarii. Lubańska przeprowadziła pogłębione wywiady i obserwację etnograficzną, dotyczącą sąsiadowania mieszkańców różnych religii, ich wierzeń i praktyk religijnych.

Epiphytic diatoms associated with red mangrove (Rhizophora mangle prop roots in Bahía Magdalena, Baja California Sur, Mexico

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D.A Siqueiros Beltrones

2006-06-01

Full Text Available The first floristic inventory of benthic diatoms is provided for the Bahía Magdalena- Bahía Almejas lagoon system. Samplings were carried out during November of 1999. The oxydized samples were mounted permanently. Eighty six diatom taxa were identified, out of which 59 are new records for the Bahía Magdalena area, and 12 taxa are new for the Baja California peninsula. Taxa recorded previously as rare in other substrata are common or abundant on the epiphytic macroalgae of mangrove prop roots. Other species are mainly epipelic forms, while 24 are commonly found as tychoplankton in the area. Certain taxa appear to be characteristic of mangrove systems in general. Rev. Biol. Trop. 54(2: 287-297. Epub 2006 Jun 01.Se presenta el primer inventario florístico de diatomeas bénticas para el Sistema Lagunar Bahía Magdalena-Bahía Almejas. Se hicieron muestreos en noviembre de 1999. En las muestras oxidadas y montadas permanentemente, se identificaron 86 táxones, de los cuales 59 son nuevos registros para el área de Bahía Magdalena, y 12 son nuevos para la península de Baja California. Táxones previamente registrados como escasos en otros sustratos son comunes o abundantes sobre macroalgas epífitas de raíces primarias de mangle. Otras species son principalmente formas epipélicas, mientras que 24 son constituyentes comunes del ticoplancton del área. Ciertos táxones parecen ser característicos de sistemas de manglar en general.

Pangahävitaja Leeson : "Ma kahetsen" / Nick Leeson ; interv. Argo Ideon

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Leeson, Nick

2007-01-01

Briti börsimaakler Nick Leeson, kes pankrotistas Baringsi panga ning hävitas 17 miljardit krooni, selgitab, miks ta ei suutnud oma tegevust lõpetada ka siis kui kahjud olid kasvanud juba hiigelsuureks

Pollution by metals and toxicity assessment using Caenorhabditis elegans in sediments from the Magdalena River, Colombia

International Nuclear Information System (INIS)

Tejeda-Benitez, Lesly; Flegal, Russell; Odigie, Kingsley; Olivero-Verbel, Jesus

2016-01-01

The Magdalena River is the most important river in Colombia, supplying over 70% of the population of fish and drinking water, and it also is the main river transportation way of the country. It receives effluents from multiple sources along its course such as contaminant agricultural and industrial discharges. To evaluate the toxicity profile of Magdalena River sediments through endpoints such as survival, locomotion, and growth, wild type strains of Caenorhabditis elegans were exposed to aqueous extracts of the sediments. To identify changes in gene expression, GFP transgenic strains were used as reporter genes. Physiological and biochemical data were correlated with metal concentration in the sediments, identifying patterns of toxicity along the course of the river. Levels of some metals such as Cd, Cu, and Ni were above TEC and PEC limits. Effects in survival, growth, and locomotion were observed in most of the samples, and changes in gene expression were evident in the genes mtl-2, sod-4, and gst-1 using fluorescence expression. Cadmium and lead were the metals which were primarily associated with sediment toxicity, and the sampling sites with the highest increased expression of stress response genes were Barrancabermeja and Girardot. However, the diverse nature of toxic profiles observed in C. elegans in the study area showed the pervasiveness of different types of discharges throughout the river system. - Highlights: • The Magdalena River has high levels of some metals such as Cd, Cu, and Ni. • Most sediment extracts affected lethality, growth, and locomotion of C. elegans. • Sediment extracts induced expression changes in mtl-2, sod-4, and gst-1. • Sediment toxicity was primarily associated with Cd and Pb. • Highest toxicity was observed for samples collected in mining and industrial areas. - In Magdalena River sediments, Cd and Pb were associated with toxicity in Caenorhabditis elegans and expression of stress response genes were related to

Estrategia de Pago a Proveedores de Pesquería Artesanal de Jaiba en el Departamento Del Magdalena

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Edwin Causado Rodriguez

2014-01-01

Full Text Available El presente artículo, pretende formular una estrategia de pago de materia prima proporcional a los rendimientos obtenidos en el proceso de carne de jaiba de una empresa procesadora del departamento del Magdalena. Acudiendo a la entrevista personal a trabajadores del área de producción, aseguramiento de la calidad, y proveedores de materia prima, sobre los factores que intervienen en la captura y procesamiento de este producto, y al análisis de registros históricos de rendimientos del proceso de los dos últimos años. Esta estrategia de pago ha de beneficiar tanto a la empresa al ofrecerle una proporcionalidad entre sus costos y precios de venta, como también al pescador, a fin de que éste aproveche el recurso en forma sostenible, a través una talla media de captura implementada por el INPA en su momento y adecuada por el INCODER y la Corporación Autónoma Regional del Magdalena – CORPAMAG. Por último, de acuerdo con los muestreos de producto realizados, existe una mayor tendencia a encontrar jaibas con el tamaño mínimo de pesca exigidos por las entidades reguladoras, lo cual facilita una proporcionalidad entre los costos de materia prima por tallas y los precios de venta de producto terminado.Palabras Clave: Jaiba; talla media de captura; pescador artesanal; aprovechamiento sostenible. Supplier Payment Strategy Handcrafted Crab Fishery in theMagdalena DepartmentAbstractThis article seeks to formulate a strategy for payment of material proportional to income in the process of a crab meat processing company in the department of Magdalena. Going to interview workers in the area of production, quality assurance, and raw material suppliers on the factors involved in the capture and processing of this product, and analysis of historical records of the process yields two recent years. This payment strategy has to benefit both the company by offering a proportionality between costs and selling prices, as well as a fisherman, so that to

Assessment of continuous oil and gas resources in the Middle and Upper Magdalena Basins, Colombia, 2017

Science.gov (United States)

Schenk, Christopher J.; Brownfield, Michael E.; Tennyson, Marilyn E.; Le, Phuong A.; Mercier, Tracey J.; Finn, Thomas M.; Hawkins, Sarah J.; Gaswirth, Stephanie B.; Marra, Kristen R.; Klett, Timothy R.; Leathers-Miller, Heidi M.; Woodall, Cheryl A.

2017-09-22

Using a geology-based assessment methodology, the U.S. Geological Survey estimated mean undiscovered, technically recoverable continuous resources of 0.45 billion barrels of oil and 1.0 trillion cubic feet of gas in the Middle and Upper Magdalena Basins, Colombia.

Reemplazo de grasa y azúcar en magdalenas. Efecto sobre las propiedades reológicas, térmicas, de textura y sensoriales

OpenAIRE

MARTÍNEZ CERVERA, SANDRA

2013-01-01

El presente trabajo de tesis se centra en el estudio de las propiedades reológicas, térmicas, de textura y sensoriales de magdalenas en las que se ha reemplazado parcial o totalmente la grasa o el azúcar. La masa de las magdalenas es una emulsión de aceite en agua compuesta por una base de harina que incorpora grasa y azúcar como ingredientes principales y, como opcionales, huevo, leche y otros componentes. El producto final se caracteriza por tener una estructura poro...

Elementos críticos sobre cooperación internacional en el Magdalena Medio colombiano

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Edgar Alberto Zamora Aviles

2013-09-01

Full Text Available El trabajo se propone aportar elementos críticos sobre la cooperación internacional, en particular sobre la cooperación europea, en el Magdalena Medio durante la primera década del siglo XXI. Partiendo de que los flujos de capital de la cooperación internacional para el desarrollo no son políticamente neutros y están condicionadospor los intereses geopolíticos y geoeconómicos de los donantes, sostenemos que la cooperación en esta región ha contribuido a consolidar nuevas espacialidades capitalistas en favor de la acumulación global y en detrimento de las propuestas de desarrollo territorial que han construido las comunidades de esta región. Este proceso ha operado bajo distintas modalidades de acumulación por desposesión en medio de la crisis humanitaria que ha generado el desarrollo del conflicto social y armado. En el Magdalena Medio uno de los escenarios de desposesión más destacados es el de la agroindustria palmera.

Applying ARIMA model for annual volume time series of the Magdalena River

OpenAIRE

Gloria Amaris; Humberto Ávila; Thomas Guerrero

2017-01-01

Context: Climate change effects, human interventions, and river characteristics are factors that increase the risk on the population and the water resources. However, negative impacts such as flooding, and river droughts may be previously identified using appropriate numerical tools. Objectives: The annual volume (Millions of m3/year) time series of the Magdalena River was analyzed by an ARIMA model, using the historical time series of the Calamar station (Instituto de Hidrología, Meteoro...

Trapping and breaking of in vivo nicked DNA during pulsed-field gel electrophoresis

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Khan, Sharik R.; Kuzminov, Andrei

2013-01-01

Pulsed field gel electrophoresis (PFGE) offers a high-resolution approach to quantify chromosomal fragmentation in bacteria, measured as percent of chromosomal DNA entering the gel. The degree of separation in PFG depends upon the size of DNA, as well as various conditions of electrophoresis, such as electric field strength (FS), time of electrophoresis, switch time and buffer composition. Here we describe a new parameter, the structural integrity of the sample DNA itself, that influences its migration through PFGs. We show that sub-chromosomal fragments containing both spontaneous and DNA damage-induced nicks are prone to breakage during PFGE. Such breakage at single strand interruptions results in artefactual decrease in molecular weight of linear DNA making accurate determination of the number of double strand breaks difficult. While breakage of nicked sub-chromosomal fragments is FS-independent, some high molecular weight sub-chromosomal fragments are also trapped within wells under the standard PFGE conditions. This trapping can be minimized by lowering the field strength and increasing the time of electrophoresis. We discuss how breakage of nicked DNA may be mechanistically linked to trapping. Our results suggest how to optimize conditions for PFGE when quantifying chromosomal fragmentation induced by DNA damage. PMID:23770235

Análisis de costes de producción con implementación de equipos automatizado tomando como ejemplo Mina Magdalena

OpenAIRE

Hernández Fuentealba, Carlos Andrés

2018-01-01

Se va a recopilar la información disponible relacionada a la primera mina de España con implementación de sistemas de automatización, Mina Magdalena. Es propiedad de MATSA y se encuentra ubicada en la localidad de Almonaster la Real, provincia de Huelva, España. Se va a realizar el estudio de la automatización alcanzada en Mina Magdalena, con la finalidad de estimar unos costos de producción, variando los costes de la operación de perforación y carga. Para el cálculo de estos costes se sup...

Kust koolil oma eelarvet veel kärpida annaks? / Maria-Magdalena Jürvetson, Aivar Saarela, Matti Martinson ... [jt.

Index Scriptorium Estoniae

2009-01-01

Küsimusele vastavad Virtsu Põhikooli direktor Maria-Magdalena Jürvetson, Avinurme Gümnaasiumi direktor Aivar Saarela, Rahumäe Põhikooli direktor Matti Martinson, Muhu Põhikooli direktor Senta Room ja Palivere Põhikooli direktor Tõnis Peikel

Clones de Hevea brasiliensis de alta productividad caracterizados por resistencia a Microcyclus ulei en jardin clonal en el magdalena medio colombiano

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Yeirme Yaneth Jaimes Suarez

2015-06-01

Full Text Available Con el objetivo de introducir en la región del magdalena medio de Colombia nuevos clones de caucho (Hevea brasiliensis (Willd. ex A. Juss. Müll. Arg. de alta productividad y con resistencia al Mal de las Hojas, se evaluaron materiales un jardín clonal establecido en Cimitarra, Colombia en la región del magdalena medio. Se evaluó la incidencia y la severidad del ataque de Microcyclus ulei (P. Henn. von Arx. según Garcia et al. (1. Se encontró resistencia completa al mal de las hojas para los clones CDC 312, FDR 4575, FDR 5597, FDR 5788 y MDF 180 y resistencia parcial para los clones FX 3864 y CDC 32. Los clones RRIM 901, PB 235 y PB 260 se mostraron como susceptibles. De acuerdo con los resultados, los clones con resistencia total y parcial se podrán recomendar para zonas preferenciales con restricción para el cultivo, una vez este comportamiento se corroboré en campo clonal. En cuanto a los clones susceptibles se pueden recomendar para zonas de escape al mal de las hojas en el Magdalena Medio Colombiano.

Obtención de secuencias microsatelitales especie especificas para Plagioscion magdalenae (Pisces:Sciaenidae

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Natalia Juliana Bayona-Vásquez

2007-08-01

en la parte baja de la cuenca del río Magdalena y en la cuenca del río San Jorge, Colombia, en donde cada vez su comercialización cobra fuerza debido al desplazamiento y disminución en los volúmenes de captura de otras especies como el bagre rayado y bocachico. Lo cual ha llevado a la comúnmente llamada pacora a catalogarse en estado de vulnerabilidad en las cuencas colombianas. Lo anterior, hace necesario la generación de información que sirva de sustento a programas de conservación y uso sostenible de la especie. Una primera aproximación a las poblaciones de P. magdalenae puede ser hecha a través de marcadores moleculares microsatelitales, útiles en trabajos de genética de poblaciones, conservación y manejo de recursos biológicos. Sin embargo, la principal dificultad de los microsatélites es que deben ser aislados de novo para aquellas especies que van a ser evaluadas por primera vez, lo que lleva a la necesidad de obtener primers para la especie. En este trabajo, inicialmente se obtuvo un ADN de buena calidad útil para amplificaciones a partir de muestras de músculo colectadas en cuatro puntos de la cuenca del río San Jorge; posteriormente, se aislaron primers microsatelitales especie-específicos para P. magdalenae mediante amplificación cruzada con primers de otras especies de peces lejanas, entre las cuales se encuentra: Pseudoplatystoma corruscans, Pimelodella chagressi, Prochilodus argenteus y Prochilodus costatus. Para lo anterior, fue necesario modificar y estandarizar protocolos de amplificación mediante cambios en temperatura de alineamiento y concentración de reactivos; y por último se realizó el secuenciamiento de regiones flanqueantes y motivos de repetición en aquellos sistemas que presentaron resultados positivos, de esta forma, se diseñaron primers de regiones microsatélite para la especie. De los trece sistemas evaluados, de las cuatro especies, se obtuvo amplificación positiva de seis. Por medio de la t

Three new species of Hemibrycon (Characiformes: Characidae from the Magdalena River Basin, Colombia

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César Román-Valencia

2013-09-01

Full Text Available Fish biodiversity of aquatic ecosystems is highly threatened by different economic activities driven by human populations, and its description is increasingly a priority. For the Cauca-Magdalena River system we have described 14 species, and the purpose of this paper was to describe three new species belonging to the same genus Hemibrycon from the Nare and Guatapé River drainages of the middle Magdalena River, Colombia. The description was based on a series of 200 specimens, and the use of morphometric, meristic and osteological characters, as well as fish distribution and morphogeometric analytical methods. We have found that Hemibrycon fasciatus n. sp. (n=54 differs from other species of Hemibrycon (that also have a vertical humeral spot in having: melanophores outlining the posterior margins of the scales along sides of body; humeral spot extending onto posterior margin of opercle; a dark lateral stripe, formed by deep pigment that is continuous with the peduncular spot; the toothed portion of the maxilla not reaching the dorsal margin of the dentary (vs. toothed portion of maxilla extending beyond dorsal margin of dentary; all maxillary teeth tricuspid (vs. some unicuspid teeth present on maxilla. H. cardalensis n. sp. (n=64 differs in having: a vertically elongate humeral spot that extends one or two scales below the lateral line canal. H. cardalensis n. sp. differs from all congeners in having the pigment of the caudal spot restricted to the ventral half of the caudal peduncle, and in having melanophores around the anterior scales of the lateral line. Hemibrycon antioquiae n. sp. (n=82 differs in having a circular humeral spot. It differs from the other species with a circular humeral spot, like H. mikrostiktos, in having a projection of disperse melanophores extending from the dorsal margin of the humeral spot to below the lateral stripe. Habitat data and environmental impacts caused by the construction of reservoirs for hydroelectric

Nick Ransford: 'There is a job to do so let's get on with it'.

Science.gov (United States)

Ransford, Nick; Doherty, Ruth

2014-03-01

Nick Ransford is a consultant in special care dentistry at Birmingham Community Healthcare NHS Trust. He has over 25 years' experience working with adults with disabilities and medical conditions across the spectrum.

Nick translation detection in situ of cellular DNA strand break induced by radiation

International Nuclear Information System (INIS)

Maehara, Y.; Anai, H.; Kusumoto, T.; Sakaguchi, Y.; Sugimachi, K.

1989-01-01

DNA strand break in HeLa cells induced by radiation was detected using the in situ nick translation method. The cells were exposed to radiation of 3, 6, 12, 18, and 24 Gy in Lab-Tek tissue culture chamber/slides and were fixed with ethanol/acetic acid on the slide glass. The break sites in DNA were translated artificially in the presence of Escherichia coli DNA polymerase I and [ 3 H]-labeled dTTP. Autoradiographic observation was made of the level of break sites in the DNA. The DNA strand break appeared even with a 3 Gy exposure, increased 8.6 times at 24 Gy compared with the control cells, and this level correlated reciprocally to change in cell viability. This nick translation method provides a rapid in situ assay for determining radiation-induced DNA damage of cultured cells, in a semi-quantitative manner

Irene Galeano: El saber de una partera tradicional del Magdalena medio Irene Galeano: the knowledge of a traditional midwife in the Magdalena zone

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Celmira Laza Vásquez

2008-12-01

Full Text Available Madeleine Leininger en su teoría enfermería transcultural, pone énfasis en la coherencia entre las intervenciones de enfermería y el sistema de valores así como el contexto cultural que rodea al sujeto de cuidado. Por tanto la enfermera debe buscar estrategias que le permitan ser sensible a la concepción que tienen las personas de sus problemas de salud con el fin de mediar entre el contexto cultural y las necesidades de cuidado de la persona. Por medio de un relato biográfico se da la oportunidad a los sujetos de cuidado de expresar la complejidad de su realidad y sobre todo la experiencia de la persona frente sus procesos de salud - enfermedad. El presente artículo tiene como objetivo narrar en la voz de su protagonista, la experiencia de vida de una partera tradicional de la región del Magdalena medio colombiano. En la narración, se entrecruzan las creencias y acervos culturales propios de la región rural del departamento de Antioquia y que determinan las prácticas de cuidado para la atención de la mujer durante el parto y el puerperio.Madeleine Leininger in her theory of multicultural infirmary emphasizes coherence, participation, system of values and patient’s treatment as well. For this reason infirmary must find strategies allowing the concept of sensibility and the awareness people have of their health problems in order to deal with their own urban context and their needs for self care through a biographic narration. The patients have the opportunity to express their true reality and mainly their own experience about health and illness - process. The purpose of this article is to reveal experiences narrated by the protagonist (an urban midwife from the Magdalena region, "a Colombian area". This true story is full of believes and unique cultural details from the rural region of the department (state of Antioquia where they set methods for woman’s care during delivery and after it.

Nick Hornby’s A Long Way Down (2005 in the Transition from Book to Movie

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Ömercan Tüm

2015-12-01

Full Text Available Nick Hornby’s A Long Way Down (2005 in the Transition from Book to movie Abstract Challenging and profound, Nick Hornby’s novel A Long Way Down (2005 is the story of four people failing to commit suicide. The protagonists are caught in an intricate web of relationships, disappointments and missed chances on their one-way journey to understand that “The cure for unhappiness is happiness” (Elizabeth McCracken. This paper aims at demonstrating that 2014 movie version directed by Pascal Chaumeil fails to capture the essence of the book and resorts to a number of radical changes which are only supposed to attract a larger audience, but do not necessarily send the same message as the novel.

Transformations of Mangrove Forests in Bahia Magdalena, Baja California Sur, Mexico: Two Decade Results Based on Landsat Imageries

Science.gov (United States)

Suresh Babu, S.; Abdul Rahaman, S.; Muthushankar, G.; Jonathan, M. P.

2014-12-01

Mangrove forests which thrive along the tropical and subtropical regions are the most productive ecosystems in the world with a wide range of ecological and economical services to mankind. With the rapid urbanization across the globe, these forests tend to be destroying at an alarming rate. The area of concern for this study, Bahia Magdalena is very important for the economy of the state as nearly 50% of the artisan fisheries are established in the mangrove zone. Henceforth this study is an attempt for a regional assessment and to accurately quantify the mangroves using LANDSAT imageries for over two decades in Bahia Magdalena, Baja California. Satellite imageries from the year 1986 through 2014 were analysed to assess the prolonged changes taking place in and around the mangrove reserve. Using the estimates of land use/cover for all the years, the spatio - temporal data was validated using ArcGIS software. The results revealed that the spatial extent of mangroves are decreasing until 2005 due to the developmental plans such as tourism, shrimp farming and establishment of industries in this part of the country. During the past 10 years (~ after 2005) there is no much change in the area extent of mangrove reserves due to afforestation and conservation efforts. Thus the unbiased dataset generated may be widely used for an improved understanding of the role of mangrove forests in the socio economic aspects, protection from natural disasters, identify possible areas for conservation, restoration and rehabilitation; and improve estimates of the amount of carbon stored in mangrove vegetation and the associated marine environment. Keywords: Mangroves, LANDSAT, Bahia Magdalena, México.

Managing water scarcity in the Magdalena river basin in Colombia.An economic assessment

Science.gov (United States)

Bolivar Lobato, Martha Isabel; Schneider, Uwe A.

2014-05-01

Key words: global change, water scarcity, river basin In Colombia, serious water conflicts began to emerge with the economic development in the 70ies and 80ies and the term "water scarcity" became a common word in this tropical country. Despite a mean annual runoff of 1840 mm, which classifies Colombia as a water rich country, shortfalls in fresh water availability have become a frequent event in the last two decades. One reason for the manifestation of water scarcity is the long-held perception of invulnerable water abundance, which has delayed technical and political developments to use water more efficiently. The Magdalena watershed is the most important and complex area in Colombia, because of its huge anthropogenic present, economic development and increasing environmental problems. This river basin has a total area of 273,459 km2, equivalent to 24% of the territory of the country. It is home to 79% of the country's population (32.5 million of inhabitants) and approximately 85% of Gross Domestic Product of Colombia is generated in this area. Since the economic development of the 1970s and 1980s, large changes in land cover and related environmental conditions have occurred in the Magdalena basin. These changes include deforestation, agricultural land expansion, soil degradation, lower groundwater and increased water pollution. To assess the consequences of geophysical alteration and economic development, we perform an integrated analysis of water demand, water supply, land use changes and possible water management strategies. The main objective of this study is to determine how global and local changes affect the balance between water supply and demand in the Magdalena river basin in Colombia, the consequences of different water pricing schemes, and the social benefits of public or private investments into various water management infrastructures. To achieve this goal, a constrained welfare maximization model has been developed. The General Algebraic Modeling

Los archivos notariales como fuente histórica:una visión desde la zona bananera del Magdalena

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Adriana Mercedes Corso

2004-01-01

Full Text Available Este artículo es una exploración metodológica e historiográfica de los registrosnotariales y surge de nuestro estudio en curso de la zona bananera delMagdalena desde 1890 hasta 1930. Nuestro propósito es alertar a los estudiantesde historia sobre algunas

Una nueva especie de Bolitoglossa (Caudata: Plethodontidae de las selvas del Magdalena Medio en Colombia

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Acosta Galvis Andrés R.

2001-12-01

Full Text Available Se describe una nueva especie del genero Bolitoglossa del grupo alfa (sensu Wake & Lynch, 1976 denominada Bolitoglossa lozanoi de las selvas húmedas relictuales del valle del Río Magdalena en La Cordillera Central de Colombia (loe. typ.: Río La Miel, Mpio. La Victoria, Departamento de Caldas, 500 m.s.n.m.; la nueva especie es reconocible por su cuerpo robusto, las palmeaduras manuales y pediales extensas, su patrón cromático ventral café con punteaduras café más oscuras y crema, ojos no protuberantes y la talla corporal relativamente grande entre las especies de salamandras del país. A new species of the genus Bolitoglossa, of the alpha group (sensu Wake & Lynch, 1976 named Bolitoglossa lozanoi from the relictual humid forest from the Magdalena Valley on the eastern slope ofthe central Andes Mountain range in Colombia (type locality: La Miel River, Mpio. La Victoria, Dept. of Caldas, 500 m.s.n.m.. The new species is mainly recognized by the presence of a robust body, extensively webbed hands and toes, a brownish ventral chromatic pattern with small darker brown and cream dots, non-protuberant eyes and a relatively large size.

El Programa de Desarrollo y Paz del Magdalena Medio y la Red Prodepaz

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María del Rosario Saavedra

2008-06-01

Full Text Available Debido al largo y complejo conflicto armado que afecta a Colombia, han surgido en las diferentes regiones organizaciones que plantean alternativas para una convivencia en paz y un desarrollo compatible con la inclusión social. El Magdalena Medio cuenta con uno de los programas más vigorosos en esta materia. En este artículo la autora comparte su experiencia en este programa y expone los principales conceptos y características metodológicas que han permitido sostener y expandir este esfuerzo colectivo durante varios años.

Analysis of social responsibility practices and actions. A case study in Cun corporation. Magdalena region

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Devinso Jiménez Sierra

2016-11-01

Full Text Available The project will analyze the policies, practices and social responsibility plans of the Unified Regional Higher Education Corporation Magdalena today, in order to promote corporate memory related to CSR through a measurement model based on eight indicators related to changing economic, social and environmental stakelholders practices. The analysis also seeks to measure the correlation between CSR practices implemented and perceived levels of the most influential stakeholders of the corporation.

Perspectives on the ‘lens of risk’ interview series: interview with Nick Pidgeon

NARCIS (Netherlands)

Heyman, B.; Brown, P.

2012-01-01

This article is the first in a series which will appear in 2012 in the special issue series Health Care Through the `Lens of Risk'. It provides a quasi-verbatim transcript of an interview with Nick Pidgeon, one of the main contributors to the social science component of The Royal Society Risk Report

HYPHESSOBRYCON NATAGAIMA (CHARACIFORMES: CHARACIDAE A NEW SPECIES FROM COLOMBIA, WITH A KEY TO THE MAGDALENA BASIN HYPHESSOBRYCON SPECIES

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CARLOS A. GARCÍA-ALZATE

2015-06-01

Full Text Available A new species, Hyphessobrycon natagaima, is described from the upper Magdalena River Basin in Colombia. It differs from all other species of Hyphessobrycon with a dark lateral stripe inhabiting the Magdalena River Basin: H. poecilioides, H. proteus and H. ocasoensis, by having eight to twelve pored lateral-line scales (vs. 14-26; four scales between the lateral line and the pelvic-fin insertions (vs. five or six; one tooth on the maxilla (vs. zero in H. poecilioides, and two to five in H. proteus; except H. ocasoensis, with one, a dark, interrupted, lateral stripe that is not in contact with the caudal peduncle spot (vs. absence of caudal spot in H. poecilioides, lateral stripe continued that is in contact with the caudal peduncle spot in H. ocasoensis. It has a rhomboid shaped caudal-peduncle spot that continues on to middle caudal-fin rays (vs. absence of caudal peduncle spot in H. poecilioides and caudal peduncle spot round and not continued on to middle caudal-fin rays in H. ocasoensis; and presence of hooks on all fins in mature males (vs. males with hooks on anal, pelvic and pectoral fins. Hyphessobrycon natagaima differs from H. ocasoensis, in addition to the above characters, by having four scale rows between the lateral line and the anal-fin origin (vs. six; three or four scale rows between the lateral line and the pelvic-fin insertions (vs. six; ten or eleven predorsal scales (vs. nine; i,9,i dorsal-fin rays (vs. ii,8,i; 18-20 branched anal-fin rays (vs. 21-22 and eleven branched pectoral-fin rays (vs. twelve. A key for the identification of Hyphessobrycon species present in the Magdalena River Basin is provided.

Assessment of The Pajarales Complex Rotiferofauna during The Rainy Season, Departamento de Magdalena, Colombia

International Nuclear Information System (INIS)

Celis, Sebastian; Mancera, Ernesto; Jenny A Leon

2008-01-01

In order to assess the potential effects of the Magdalena River recommunication with its former delta, we studied the abundance and composition of the rotiferofauna of the Pajarales Complex (PC) during its highest annual zooplankton abundance. We selected the Rotifera phylum, due to its high reproductive rates and the efficiency in energetic transformation process. We sampled the rotifers with a van Dorn bottle, during the second rainy season in 2006. During each sampling we registered salinity, temperature, pH, and dissolved oxygen. We calculated the rotifer diversity using the Shannon-Wiener H (log10) index comparing among sampling sites and sampling dates to establish variations along the study period. The physico-chemical variables were graphically related with the diversity values calculated using 0.05 for ??and 95% for the confidence interval. We found a total of 20 morpho types belonged to Brachionidae, Lecanidae, Filiniidae, Synchaetidae, exarthriidae and Testudinellidae families, been Brachionidae and Lecanidae the most abundant. The relationships between diversity and physico-chemical variables, show salinity as the main responsible of the rotifer diversity; in conclusion, the current rotiferofauna in the PC is most abundant and diverse than 16 years ago, before the recommunication with the Magdalena River. Likewise, dissolved oxygen concentration, pH and salinity of the CP waters were different. Taking into account that salinity was the most influent factor in the rotiferofauna diversity; the river recomommunication probably favored the increment of rotiferofauna diversity in the PC

Land legislation and its impact on the development of cattle farms in the Antioquian Magdalena Medio from 1920 to 1940

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José Roberto Álvarez Múnera

2015-06-01

Full Text Available The development of the Colombian agricultural sector, predominantly rural country, has combined two models: one led by rural entrepreneurs and one that has been promoted by the rural economy. The two have survived to the debate on agricultural policy that sought the legalization and use of land, access to credit and productive development in general. This historical reflection with support regional sources, a policy associate for the legalization of public lands in relation to business development in rural sector: Livestock in Middle Magdalena in the department of Antioquia in the first half of the twentieth century. The farm and rural production unit is understood as rural company and the landowner as an entrepreneur. In the end, it is presented the magnitude of farms in the region and period studied. This product of the doctoral thesis “Rural enterprises in Antioquia: the case of livestock in the Eastern and Middle Magdalena in the department of Antioquia, 1920-1960.

Ciénaga (Magdalena, una región administrativa o municipio de enclave

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Luis Humberto Hernández

2006-01-01

Full Text Available El análisis sobre las regiones de enclave, el caso del municipio de Ciénaga, en el Magdalena, nos permitió concluir que su proceso de modernización estuvo determinado por la presencia de la empresa multinacional bananera United Fruit Company. La multinacional bananera fue la que introdujo en la región el trabajo asalariado moderno, dislocando las formas serviles y premodernas existentes. Al atraer la mano de obra servil regional, que utilizaban las haciendas ganaderas, y convertirla en mano de obra asalariada, fundamentó sus procesos socioeconómicos y político administrativo modernos.

Un río difícil. El Magdalena: historia ambiental, navegabilidad y desarrollo

OpenAIRE

Germán Márquez Calle

2016-01-01

El río Magdalena fue por siglos la principal y casi obligada vía de comunicación desde el interior de Colombia con el Caribe y el mundo, a pesar de que su navegación siempre fue difícil como se muestra en este artículo, basado en testimonios de viaje y otras fuentes. Las dificultades, atribuibles a causas naturales como lo empinado y erosionable de la cuenca, que propician formación de bancos de arena y mucho torrencial, se agravan luego por la deforestación...

Nicked apomyoglobin: a noncovalent complex of two polypeptide fragments comprising the entire protein chain.

Science.gov (United States)

Musi, Valeria; Spolaore, Barbara; Picotti, Paola; Zambonin, Marcello; De Filippis, Vincenzo; Fontana, Angelo

2004-05-25

Limited proteolysis of the 153-residue chain of horse apomyoglobin (apoMb) by thermolysin results in the selective cleavage of the peptide bond Pro88-Leu89. The N-terminal (residues 1-88) and C-terminal (residues 89-153) fragments of apoMb were isolated to homogeneity and their conformational and association properties investigated in detail. Far-UV circular dichroism (CD) measurements revealed that both fragments in isolation acquire a high content of helical secondary structure, while near-UV CD indicated the absence of tertiary structure. A 1:1 mixture of the fragments leads to a tight noncovalent protein complex (1-88/89-153, nicked apoMb), characterized by secondary and tertiary structures similar to those of intact apoMb. The apoMb complex binds heme in a nativelike manner, as given by CD measurements in the Soret region. Second-derivative absorption spectra in the 250-300 nm region provided evidence that the degree of exposure of Tyr residues in the nicked species is similar to that of the intact protein at neutral pH. Also, the microenvironment of Trp residues, located in positions 7 and 14 of the 153-residue chain of the protein, is similar in both protein species, as given by fluorescence emission data. Moreover, in analogy to intact apoMb, the nicked protein binds the hydrophobic dye 1-anilinonaphthalene-8-sulfonate (ANS). Taken together, our results indicate that the two proteolytic fragments 1-88 and 89-153 of apoMb adopt partly folded states characterized by sufficiently nativelike conformational features that promote their specific association and mutual stabilization into a nicked protein species much resembling in its structural features intact apoMb. It is suggested that the formation of a noncovalent complex upon fragment complementation can mimic the protein folding process of the entire protein chain, with the difference that the folding of the complementary fragments is an intermolecular process. In particular, this study emphasizes the

Securing wilderness landscapes in South Africa : Nick Steele, private wildlife conservancies and saving rhinos

NARCIS (Netherlands)

Wels,; H.,

2015-01-01

Private wildlife conservation is booming business in South Africa! Nick Steele stood at the cradle of this development in the politically turbulent 1970s and 1980s, by stimulating farmers in Natal (now KwaZulu-Natal) to pool resources in order to restore wilderness landscapes, but at the same time

Dehalogenation of chloroalkanes by nickel(i) porphyrin derivatives, a computational study.

Science.gov (United States)

Szatkowski, L; Hall, M B

2016-11-14

The nickel(i) octaethylisobacteriochlorin anion ([OEiBCh-Ni (I) ] - ) is commonly used as a synthetic model of cofactor F 430 from Methyl-Coenzyme M Reductase. In this regard, experimental studies show that [OEiBCh-Ni (I) ] - can catalyze dehalogenation of aliphatic halides in DMF solution by a highly efficient S N 2 reaction. To better understand this process, we constructed theoretical models of the dehalogenation of chloromethane by a simple nickel(i) isobacteriochlorin anion and compared its reactivity with that of similar Ni (I) complexes with other porphyrin-derived ligands: porphyrin, chlorin, bactreriochlorin, hexahydroporphyrin and octahydroporphyrin. Our calculations predict that all of the porphyrin derivative's model reactions proceed through low-spin complexes. Relative to the energy of the separate reactants the theoretical activation energies (free-energy barriers with solvation corrections) for the dehalogenation of chloromethane are similar for all of the porphyrin derivatives and range for the different functionals from 10-15 kcal mol -1 for B3LYP to 5-10 kcal mol -1 for M06-L and to 13-18 kcal mol -1 for ωB97X-D. The relative free energies of the products of the dehalogenation step, L-Ni-Me adducts, have a range from -5 to -40 kcal mol -1 for all functionals; generally becoming more negative with increasing saturation of the porphyrin ligand. Moreover, no significant differences in the theoretical chlorine kinetic isotope effect were discernable with change of porphyrin ligand.

Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate.

Science.gov (United States)

Nandakumar, Jayakrishnan; Nair, Pravin A; Shuman, Stewart

2007-04-27

NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria and essential for growth. Their distinctive substrate specificity and domain organization vis-a-vis human ATP-dependent ligases make them outstanding targets for anti-infective drug discovery. We report here the 2.3 A crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition. LigA envelopes the DNA within a protein clamp. Large protein domain movements and remodeling of the active site orchestrate progression through the three chemical steps of the ligation reaction. The structure inspires a strategy for inhibitor design.

Rhombic-Shaped Nanostructures and Mechanical Properties of 2D DNA Origami Constructed with Different Crossover/Nick Designs.

Science.gov (United States)

Ma, Zhipeng; Huang, Yunfei; Park, Seongsu; Kawai, Kentaro; Kim, Do-Nyun; Hirai, Yoshikazu; Tsuchiya, Toshiyuki; Yamada, Hirofumi; Tabata, Osamu

2018-01-01

DNA origami methods enable the fabrication of various nanostructures and nanodevices, but their effective use depends on an understanding of their structural and mechanical properties and the effects of basic structural features. Frequency-modulation atomic force microscopy is introduced to directly characterize, in aqueous solution, the crossover regions of sets of 2D DNA origami based on different crossover/nick designs. Rhombic-shaped nanostructures formed under the influence of flexible crossovers placed between DNA helices are observed in DNA origami incorporating crossovers every 3, 4, or 6 DNA turns. The bending rigidity of crossovers is determined to be only one-third of that of the DNA helix, based on interhelical electrostatic forces reported elsewhere, and the measured pitches of the 3-turn crossover design rhombic-shaped nanostructures undergoing negligible bending. To evaluate the robustness of their structural integrity, they are intentionally and simultaneously stressed using force-controlled atomic force microscopy. DNA crossovers are verified to have a stabilizing effect on the structural robustness, while the nicks have an opposite effect. The structural and mechanical properties of DNA origami and the effects of crossovers and nicks revealed in this paper can provide information essential for the design of versatile DNA origami structures that exhibit specified and desirable properties. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Proposal for inclusion of ICT in the community of the riverside of river Magdalena in Girardot, Colombia

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Elkin Oswaldo Forero Soto

2016-12-01

Full Text Available The means used to appropriation of information and communications technology in the town on the banks of the Magdalena River in Girardot were not the best despite national policies, on the other hand the population is perceived increasingly distant from advances technological and with fewer opportunities. This paper proposes the design of a technology strategy for this type of community approach to information and communication technologies to reduce the digital divide.

A preliminary evaluation of shallow-water rhodolith beds in Bahia Magdalena, Mexico

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Enrique Ávila

2011-12-01

Full Text Available The aim of the present study was to describe the structure of shallow-water rhodolith beds from Bahia Magdalena, one of the most productive estuarine systems of the Mexican Pacific coasts. From September 2008 to May 2009 four rhodolith beds were found (between 1 and 3 m depth and population descriptors such as rhodolith density, size classes, branch density, volume and weight were determined. The dominant rhodolith forming species was Lithophyllum margaritae. The size of beds ranged from 7,600 to 17,800 m2 approximately with densities from 42.2 to 215.9 ind.m-2. In these beds, L. margaritae shows fruticose and foliose growth forms, from which spherical forms were predominant (81-99%. Branch density (from 3.0 to 13.3 branches.cm-2 varied significantly (p O objetivo do presente estudo foi descrever a estrutura de bancos de rodolitos de áreas rasas da Bahia Magdalena, um dos sistemas estuarinos mais produtivos da costa pacífica mexicana. Quatro bancos de rodolitos situados entre 1 e 3m de profundidade foram avaliados em relação a densidade, classes de tamanho, densidade dos ramos, volume e peso no período de setembro de 2008 a maio de 2009. A espécie dominante no local foi Lithophyllum margaritae. O tamanho dos bancos variou de 7,600 a 17,800 m² aproximadamente, com densidades de 42.2-215.9 ind.m-2 . Foram observadas formas de crescimento fruticosa e folhosa em L. margaritae, sendo a forma esférica predominante (81-99%. A densidade dos ramos (3.0-13.3 ramos.cm-2 variou significativamente (p < 0.05 entre os bancos. O volume médio (2.0-400 ml e o peso úmido (32.4-84.8 g não tiveram diferenças significativas entre os locais, mas uma correlação positiva significativa (r = 0.95, p < 0.05 foi encontrada entre os parâmetros. O tamanho das plantas variou de 2.0 a 11.5 centímetros predominando o padrão entre 40.1-60 mm. As diferenças na densidade de rodolitos, densidade dos ramos e a esfericidade foram atribuídas às condições hidrodin

Una nueva especie de bolitoglossa (caudata: plethodontidae) de las selvas del magdalena medio en colombia

OpenAIRE

Acosta Galvis, Andrés R.; Restrepo, Ana E.

2010-01-01

Se describe una nueva especie del genero Bolitoglossa del grupo alfa (sensu Wake & Lynch, 1976) denominada Bolitoglossa lozanoi de las selvas húmedas relictuales del valle del Río Magdalena en La Cordillera Central de Colombia (loe. typ.: Río La Miel, Mpio. La Victoria, Departamento de Caldas, 500 m.s.n.m.); la nueva especie es reconocible por su cuerpo robusto, las palmeaduras manuales y pediales extensas, su patrón cromático ventral café con punteaduras café más oscuras y crema, ojos no pro...

CUENTOS QUE NO SON CUENTOS...UN RECORRIDO POR SAN ZENÓN (MAGDALENA

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Tania Ximena Salcedo Goméz

2013-07-01

Full Text Available Es de tarde y el sol inclemente de febrero se refleja sobre Las cálidas aguas del río Magdalena; sus visos en naranja y rojo se inmortalizan en el tapiz del cielo, mientras en tierra, esta gama de luz iridescente se forja en la piel de los hombres y mujeres de San Zenón. Los árboles desnudos han sido bañados por el frío invierno. Las ciénagas y el río han dejado sus marcas a través del marrón 330 de sus cortezas. Las pocas hojas que quedan son solo un breve recuerdo de un amanecer con sombra. El asfalto y la pasividad del viento, quienes amenazan la lucidez.

Evolución de las características socioeconómicas de los pescadores de Ciénaga, Magdalena

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Luis Gabriel Durán-Fernández

2016-01-01

Full Text Available Esta investigación analiza la evolución socioeconómica de los pescadores artesanales del municipio de Ciénaga (Magdalena. La información fue recopilada  mediante la realización de dos encuestas, una en el año 2011 (397 perteneciente a un proyecto interno de la Universidad del Magdalena y la otra en el periodo 2014 (199, que permitieron construir una base de datos con información social y económica de esta población objeto de estudio. Los resultados analizados demostraron que no hay grandes avances en la situación socioeconómica de los pescadores artesanales y su núcleo familiar, en el cual los diferentes módulos de las encuestas demostraron estancamientos y desmejora en los diversos niveles de educación alcanzados por los hijos y cónyuges de los encuestados, además un deterioro en la forma de eliminación de desechos porque existe un aumento en la cantidades de hogares que afirmaron que la queman o la vierten en el rio y la ciénaga.

Environmental impact reduction through ecological planning at Bahia Magdalena, Mexico.

Science.gov (United States)

Malagrino, Giovanni; Lagunas, Magdalena; Rubio, Alfredo Ortega

2008-03-01

For analyzing basic marine and coastal characteristics we selected the potential sites where shrimp culture could be developed in a large coastal zone, Bahia Magdalena, Baja California Sur, Mexico. Based on our analysis, 6 sites were preselected and field stages of work were then developed to assess the precise suitability of each site in order to develop the proposed aquaculture activities. In ranking the suitability we were able to recommend the most appropriate places to develop shrimp culture in this region. Also, knowing the exact biological, physico-chemical and social environment, we determined the best species to cultivate, the recommended total area and the methodology to be used to lessen the environmental impact and to obtain the maximum profitability Our methodology could be used not only to select appropriate sites for shrimp culture in other coastal lagoons, but it also could be applied to assess the suitability in a quick and accurate way, of any other production activity in coastal zones.

Local magnitude scale for Valle Medio del Magdalena region, Colombia

Science.gov (United States)

Londoño, John Makario; Romero, Jaime A.

2017-12-01

A local Magnitude (ML) scale for Valle Medio del Magdalena (VMM) region was defined by using 514 high quality earthquakes located at VMM area and inversion of 2797 amplitude values of horizontal components of 17 stations seismic broad band stations, simulated in a Wood-Anderson seismograph. The derived local magnitude scale for VMM region was: ML =log(A) + 1.3744 ∗ log(r) + 0.0014776 ∗ r - 2.397 + S Where A is the zero-to-peak amplitude in nm in horizontal components, r is the hypocentral distance in km, and S is the station correction. Higher values of ML were obtained for VMM region compared with those obtained with the current formula used for ML determination, and with California formula. With this new scale ML values are adjusted to local conditions beneath VMM region leading to more realistic ML values. Moreover, with this new ML scale the seismicity caused by tectonic or fracking activity at VMM region can be monitored more accurately.

[Multiple-Victimisation due to Armed Conflict and Emotional Distress in the State of Magdalena, Colombia].

Science.gov (United States)

Campo-Arias, Adalberto; Sanabria, Adriana R; Ospino, Anyelly; Guerra, Valeria M; Caamaño, Beatriz H

Emotional distress is common in Colombian armed conflict victims. Multiple-victimisation is associated with an increase in emotional distress than victimisation due a single event. However, the association between poly-victimisation and emotional distress among victims of the armed conflict in Colombia has not been documented. To study the association between multiple-victimisation and emotional distress in victims of armed conflict in the State of Magdalena, Colombia. A cross-sectional study was designed, with a secondary analysis of registration of the Psychosocial Care Program and Victim Integral Health (PAPSIVI) in the State of Magdalena, from 2013 to 2014. The profile formula grouped demographic variables, victimising events, and a set of symptoms of emotional distress (perceived discrimination, depressive and anxiety-stress). Odds ratio (OR, 95%CI) were established as measures of association. A total of 943 people were included, with 67.4% women, and ages between18 and 94 years (mean 47.9±14.2). A total of 109 (11.7%) suffered from multiple victimisation. Multiple victimisation events were associated with more emotional distress, depressive symptoms (OR=1.5; 95%CI, 1.3-1.8), perceived stigma (OR=1.3; 95%CI, 1.1-1.5), and anxiety-stress (OR=1.2; 95%CI 1.0-1.4) than a single event. There is more emotional distress in multiple victimisations than in a single victimisation event during armed conflict in this region of Colombia. Further studies are required on this topic. Copyright © 2016 Asociación Colombiana de Psiquiatría. Publicado por Elsevier España. All rights reserved.

Programa de orientación y consejería a estudiantes y padres de familia del programa de enfermeria de la Universidad del Magdalena

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Tatiana González Noguera

2013-10-01

Full Text Available ResumenEl presente Programa de Orientación Terapéutica a Padres de Familia y Estudiantes del Programa de Enfermería de la Universidad del Magdalena tiene por objetivo restablecer el equilibrio emocional del estudiante, como medio para ayudarlo en el enfrentamiento de las crisis, contribuir a la solución del problema y su crecimiento personal; actualmente pretende fomentar la interacción con los padres de familia y los estudiantes a través de encuentros periódicos institucionales directos e indirectos que estimulen la convivencia pacifica en el ámbito intrafamiliar, lo cual redundan en el buen rendimiento académico y crecimiento personal de los estudiantes. Este proyecto ha despertado el interés de diferentes ambientes académicos de la Universidad del Magdalena los cuales se han vinculado desarrollando softwares especiales a fin de facilitar el registro de actividades e informes estadísticos. Los registros desde su inicio en el segundo semestre de 2004, evidencian la asistencia del 64% de los padres de familia a los talleres y del 87% de los estudiantes del programa a diferentes tipos de intervención individual o grupal. (Duazary 2007; 1: 71 - 78AbstractThe present Program of Therapeutic Orientation Family parents and Students of the Program of Nursing of the University of Magdalena has for aim restore the emotional balance of the student, helping it in the clash of the crises, contributing to the solution of the problem and personal growth; simultaneously that foments the interaction with the family parents and the students across periodic institutional direct and indirect meetings that stimulate the living together appeases in the area intrafamiliar(family, redounding to the good academic performance(yield and personal growth of the students. This project to woken up the interest of different academic environments of the University of Magdalena which have linked themselves developing software in order to facilitate the record of

Siim Nestor soovitab : Jus Fine ja DJ Hype. Mutant ja Nick Luscombe. Kuurorti kogumik / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2005-01-01

Inglise drum'n'bass-staar DJ Hype 17. märtsil klubis Privé üritusel "Jus' Fine". Inglise diskor Nick Luscombe üritusel "Mutant" 18. märtsil klubis Privé. Plaadifirma Kuurortrecords esitleb 18. märtsil oma uut kogumikplaati Pirita jõe suudmes asuval laeval Monica

An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification.

Science.gov (United States)

Lin, Zhenyu; Yang, Weiqiang; Zhang, Guiyun; Liu, Qida; Qiu, Bin; Cai, Zongwei; Chen, Guonan

2011-08-28

A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

¿Para dónde va el Magdalena? : elementos sobre logística y transporte verde

OpenAIRE

Duque Escobar, Gonzalo

2015-01-01

Algunos elementos sobre logística y transporte verde, preparados a nombre de la SMP-UN, para aportar al conocimiento de las problemáticas sociales, ambientales y económicos del proyecto de navegabilidad del río Magdalena, cuya navegabilidad exige, además de considerar las limitantes ecológicas del río y su lamentable estado relacionado con la deforestación de cuencas como causa estructural y primera de la sedimentación, estructurar un sistema intermodal de transporte de carga para la Región A...

Conditions of fertility of the soils of the banana Area; Magdalena Department

International Nuclear Information System (INIS)

Bonilla Correa, Carmen Rosa; Garcia Ocampo, Alvaro

2000-01-01

The banana tree production in Colombia constitutes an important line of the economy of the country; it participates actively in the generation of foreign currencies for the high export volumes. However, in the production process, exist a series of inconveniences that if they are not managed appropriately they can affect the production directly. One of those problems is the fertilization of the plantations, the appropriate handling of the soils and the fertilization plans. With the objective of establishing the nutritional state of the soils of the banana area of the Magdalena, was carried out the present study with the purpose of determining the percentage distribution of the soils inside the different ranges of nutritious elements. Equally, with the analysis it could settle down the faulty elements in those soils and the importance of incorporating them appropriately in the fertilization plans

Estudio para la medición de seguridad alimentaria y nutricional en el magdalena medio colombiano. caso cesar.

OpenAIRE

Del Castillo M., Sara; Fonseca, Zulma; Mantilla, Mariela; Mendieta, Néstor

2012-01-01

Antecedentes. El presente artículo presenta los resultados del Estudio para la Medición de la Seguridad Alimentaria y Nutricional  (SAN) en el Magdalena Medio Colombiano. Caso César, realizado por Obusinga y el OBSAN, financiado por OBUSINGA y por la UGI de la Facultad de Medicina de la Universidad Nacional de Colombia. Adicionalmente, forma parte de las acciones del Proyecto de Construcción Participativa de una Propuesta de Desarrollo Territorial con Perspectiva Alimentaria en 12 Municipios ...

DNA fragments assembly based on nicking enzyme system.

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Rui-Yan Wang

Full Text Available A couple of DNA ligation-independent cloning (LIC methods have been reported to meet various requirements in metabolic engineering and synthetic biology. The principle of LIC is the assembly of multiple overlapping DNA fragments by single-stranded (ss DNA overlaps annealing. Here we present a method to generate single-stranded DNA overlaps based on Nicking Endonucleases (NEases for LIC, the method was termed NE-LIC. Factors related to cloning efficiency were optimized in this study. This NE-LIC allows generating 3'-end or 5'-end ss DNA overlaps of various lengths for fragments assembly. We demonstrated that the 10 bp/15 bp overlaps had the highest DNA fragments assembling efficiency, while 5 bp/10 bp overlaps showed the highest efficiency when T4 DNA ligase was added. Its advantage over Sequence and Ligation Independent Cloning (SLIC and Uracil-Specific Excision Reagent (USER was obvious. The mechanism can be applied to many other LIC strategies. Finally, the NEases based LIC (NE-LIC was successfully applied to assemble a pathway of six gene fragments responsible for synthesizing microbial poly-3-hydroxybutyrate (PHB.

Petroleum systems of the Upper Magdalena Valley, Colombia

Energy Technology Data Exchange (ETDEWEB)

Sarmiento, L.F.; Rangel, A. [Instituto Colombiano del Petroleo, Bucaramanga (Colombia). ECOPETROL

2004-03-01

In the Upper Magdalena Valley, Colombia, four petroleum systems were identified. Two petroleum systems are located in the Girardot sub-basin and the other two in the Neiva sub- basin. Limestones laterally changing to shales of the lower part of the Villeta Gp, deposited during Albian and Turonian marine flooding events, constitutes the main source rocks of the oil families. These rocks contain 1-4% TOC and type II kerogen. The littoral quartz arenites of the Caballos (Albian) and Monserrate (Maastrichtian) Fms. are the main reservoir rocks. Seal rocks are Cretaceous and Paleocene shales. Overburden includes the Cretaceous rocks and the Tertiary molasse deposited simultaneously with development of two opposite verging thrust systems during Cenozoic time. These deformation events were responsible for trap creation. Except for the Villarrica area, where the source rock reached maturity during the Paleocene, generation occurred during Miocene. Two oil families are identified, each in both sub-basins: One derived from a clay-rich source and the second from a carbonate-rich source rock lithofacies of the lower part of Villeta Gp. Geochemical source-rock to oil correlations are demonstrated for the three of the petroleum systems. Up-dip lateral migration distances are relatively short and faults served as vertical migration pathways. A huge amount of oil was probably degraded at surface, as a result of Miocene deformation and erosion. (author)

Reproduction and conservation of the Magdalena River turtle (Podocnemis lewyana) in the Claro Cocorna Sur River, Colombia; Reproduccion y conservacion de la tortuga del Rio Magdalena (Podocnemis lewyana) en el Rio Claro Cocorna Sur, Colombia

Energy Technology Data Exchange (ETDEWEB)

Ceballos, Claudia P; Romero, Isabel; Gomez Saldarriaga, Catalina; Miranda, Karla

2014-07-01

The Magdalena river turtle, Podocnemis lewyana, is an endangered and endemic turtle from Colombia. Among the most important information needed to conserve endangered species is to identify, monitor, and protect the sites used by the species to reproduce and grow. In this study we report, for the first time, the reproductive output and the nesting beaches of P. lewyana in the Claro Cocorna Sur River, a tributary of the Magdalena river drainage. We systematically examined a river transect of 8 km with 14 sandy beaches during two nesting seasons in one year. We recorded a yearly production of 47 clutches, 957 eggs, and two preferred nesting beaches: Alto Bonito with 51 %, and Belgica with 28.3 % of this reproductive output. Aafuver, a community-based organization, has led a headstarting program since 2010 to decrease in-situ egg mortality due to predation on nesting beaches. Aafuver collects and incubates the eggs ex-situ, raises the hatchlings for one to five months and then releases them into the same river. To understand potential effects of such egg manipulation, we monitored and compared in-situ and ex-situ incubation temperatures. We found ex-situ temperatures below the pivotal temperature known for P. lewyana and below the temperatures in nesting beaches. Finally, we monitored hatchlings growth under aafuver captive conditions, and found that hatchlings duplicated their body mass during the first three months of age. Egg weight was strongly associated to body weight at hatching; however this association is lost by the third month of age. We strongly encourage supporting this community-based conservation program, and the protection of the Claro Cocorna Sur River as an important nesting and growth habitat for the conservation of P. lewyana.

The wetlands of Magdalena medio Antioqueno from a physical and socio-cultural perspective

International Nuclear Information System (INIS)

Caballero Acosta, Humberto; Durango Lopez, Consuelo; Giraldo Castro, Carlos Augusto

2001-01-01

Wetlands are ecosystems with environmental and ecological importance that require of a new methodological and legal focus that allows developing conservation policies in agreement with the natural and socio-cultural conditions. In the Magdalena Medio Antioqueno, CORANTIOQUIA region, were recognized 362 wetlands, divided in two main groups; in Puerto Nare and Puerto Berrio to the south prevails herbaceous wetlands without permanent surface water, locally known as Bajos. In Yondo the wetlands have permanent surface water. The environmental dynamic depends from the geomorphologic and hydrological conditions and are affected in a differential way by the human action. The region is the result of a long occupation process that begins in prehistoric times and had changed depending on the different historical moments. This complex social configuration has given origin to diverse perceptions and types of appropriation of the wetlands what explains the identified environmental circumstances

Exploratory Analysis of Dengue Fever Niche Variables within the Río Magdalena Watershed

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Austin Stanforth

2016-09-01

Full Text Available Previous research on Dengue Fever have involved laboratory tests or study areas with less diverse temperature and elevation ranges than is found in Colombia; therefore, preliminary research was needed to identify location specific attributes of Dengue Fever transmission. Environmental variables derived from the Moderate Resolution Imaging Spectroradiometer (MODIS and Tropical Rainfall Measuring Mission (TRMM satellites were combined with population variables to be statistically compared against reported cases of Dengue Fever in the Río Magdalena watershed, Colombia. Three-factor analysis models were investigated to analyze variable patterns, including a population, population density, and empirical Bayesian estimation model. Results identified varying levels of Dengue Fever transmission risk, and environmental characteristics which support, and advance, the research literature. Multiple temperature metrics, elevation, and vegetation composition were among the more contributory variables found to identify future potential outbreak locations.

The DNA Repair Repertoire of Mycobacterium smegmatis FenA Includes the Incision of DNA 5' Flaps and the Removal of 5' Adenylylated Products of Aborted Nick Ligation.

Science.gov (United States)

Uson, Maria Loressa; Ghosh, Shreya; Shuman, Stewart

2017-09-01

We characterize Mycobacterium smegmatis FenA as a manganese-dependent 5'-flap endonuclease homologous to the 5'-exonuclease of DNA polymerase I. FenA incises a nicked 5' flap between the first and second nucleotides of the duplex segment to yield a 1-nucleotide gapped DNA, which is then further resected in dinucleotide steps. Initial FenA cleavage at a Y-flap or nick occurs between the first and second nucleotides of the duplex. However, when the template 3' single strand is eliminated to create a 5'-tailed duplex, FenA incision shifts to between the second and third nucleotides. A double-flap substrate with a mobile junction (mimicking limited strand displacement synthesis during gap repair) is preferentially incised as the 1-nucleotide 3'-flap isomer, with the scissile phosphodiester shifted by one nucleotide versus a static double flap. FenA efficiently removes the 5' App(dN) terminus of an aborted nick ligation reaction intermediate, thereby highlighting FenA as an agent of repair of such lesions, which are formed under a variety of circumstances by bacterial NAD + -dependent DNA ligases and especially by mycobacterial DNA ligases D and C. IMPORTANCE Structure-specific DNA endonucleases are implicated in bacterial DNA replication, repair, and recombination, yet there is scant knowledge of the roster and catalytic repertoire of such nucleases in Mycobacteria This study identifies M. smegmatis FenA as a stand-alone endonuclease homologous to the 5'-exonuclease domain of mycobacterial DNA polymerase 1. FenA incises 5' flaps, 5' nicks, and 5' App(dN) intermediates of aborted nick ligation. The isolated N-terminal domain of M. smegmatis Pol1 is also shown to be a flap endonuclease. Copyright © 2017 American Society for Microbiology.

Análisis funcional de la red trófica de Bahía Magdalena Baja California Sur, México

OpenAIRE

Cruz-Escalona,Víctor H; Morales-Zárate,María V; Navia,Andrés F; Rguez-Baron,Juan M; del Monte-Luna,Pablo

2013-01-01

El objetivo del presente estudio fue desarrollar un modelo trófico (ECOPATH con ECOSIM) para caracterizar la estructura y función de la trama alimentaria de Bahía Magdalena. El modelo consta de 24 grupos funcionales, siendo dominado por grupos de niveles tróficos secundarios y terciarios, que generan un tercio de los flujos de biomasa total. Los flujos totales del sistema y la eficiencia de transferencia promedio entre niveles tróficos, encajan bien en el rango reportado para otros ecosistema...

Relationship between soil and edaphoclimatic properties and the Black Sigatoka incidence (Mycosphaerella fijiensis Morelet in the Banana Region of Magdalena - Colombia

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Sonia Esperanza Aguirre Forero

2012-10-01

Full Text Available Black Sigatoka (Mycosphaerella fijiensis Morelet disease is the greatest limiting factor affecting banana crop production across the world. The pathogen´s severity has reached epidemic levels and is exacerbated by the cultivation of monocultures and genetically uniform clones. In Colombia, losses occur in the exporting regions of Uraba and Magdalena, where its management depends on the use of agrochemicals, without achieving eradication. In the search for methods to reduce the pathogen’s effect within the banana zone of the departamento del Magdalena, Colombia, the relationship between climate, soil properties, and the presence of the disease was determined. Were utilized data from geographic informational systems, digitalized the data from the soil variables reported in the soil study conducted by IGAC (2009, records from weather stations in the region, and data showing the incidence rate of the pathogen. As a result of the study were established three areas (high, medium and low incidence rate and discovered a positive correlation between the incidence rate of the disease with precipitation (r = 0.56, interchangeable magnesium, Mg+2 (r = 0.45, microporosity (r = 0.40, clay content (r = 0.54, and evaporation (r = 0.51. Were observed that there are soil conditions that influence the presence of the disease, variables that should be kept in mind in the management of banana cultivation.

Isothermal amplification detection of nucleic acids by a double-nicked beacon.

Science.gov (United States)

Shi, Chao; Zhou, Meiling; Pan, Mei; Zhong, Guilin; Ma, Cuiping

2016-03-01

Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use. Copyright © 2015 Elsevier Inc. All rights reserved.

Distribution patterns of the barnacle, Chelonibia testudinaria, on juvenile green turtles (Chelonia mydas in Bahia Magdalena, Mexico Patrones de distribución del balano, Chelonibia testudinaria, en tortugas verdes (Chelonia mydas juveniles en bahía Magdalena, México

Directory of Open Access Journals (Sweden)

Eduardo Nájera-Hillman

2012-12-01

Full Text Available The barnacle, Chelonibia testudinaria, is an obligate commensal of sea turtles that may show population variability according to the physical characteristics of the environment and properties of turtle hosts; therefore, we characterized the distributional patterns and the potential effects on health of C. testudinaria on juvenile green turtles in Bahia Magdalena, Mexico. Barnacle attachment position, abundance, and size-class structure were described, while the relationship between barnacle abundance and turtle health condition and size was explored through generalized linear models. Juvenile green turtles of Bahía Magdalena offered an appropriate habitat for C. testudinaria as their frequency of occurrence was high and their abundance was similar to that found in other green turtle populations. Barnacles showed an aggregated distribution that may be facilitating their reproduction during the warm summer months. Barnacle abundance is unaffected by turtle size or seasonality, while their attachment position appears to be influenced by water flow over the turtle carapace and by plastron abrasion by the turtle against the sea floor. Healthier turtles exhibited a decreased barnacle load; therefore we suggest that C. testudinaria abundance may be a useful indicator of health for juvenile green turtles.El balano Chelonibia testudinaria es un comensal obligado de tortugas marinas que puede presentar variaciones poblacionales de acuerdo a características ambientales y de las tortugas que coloniza; por lo tanto, caracterizamos su distribución y el efecto sobre la salud de tortugas verdes (Chelonia mydas juveniles en bahía Magdalena, México. Se describieron la posición de adherencia, la abundancia y la estructura de tallas de C. testudinaria, mientras que la relación entre su abundancia y la salud y el tamaño de las tortugas se exploró a través de modelos lineales generalizados. Las tortugas verdes ofrecieron un hábitat adecuado para C

Magistritööde kaitsmine : [Maria-Magdalena Jürvetson jt.

Index Scriptorium Estoniae

1999-01-01

14. juunil '99 kaitsevad TPÜ filoloogiateaduskonna magistritööde kaitsmisnõukogu koosolekul Maria-Magdalena Jürvetson magistritöö "Soome keele ja rahvuskultuuri areng 19. sajandil" ja Hilve Rebase magistritöö "Norra ja Soome kirjanike loomingust ja retseptsioonist Eestis"; 15. juunil kaitsevad TPÜ infoteaduste osakonna magistritööde kaitsmisnõukogu koosolekul Aivi Sepp magistritöö "Raamatukogu- ja infoteaduslik haridus 20. sajandi teisel poolel" ja Marianne Paimre magistritöö "Narkotemaatika Eesti loetavamates päevalehtedes aastatel 1993-1995 : bibliomeetriline aspekt"; 17. juunil kaitsevad TPÜ geoökoloogia magistritööde kaitsmisnõukogu koosolekul Raimo Pajula magistritöö "Raba mikromaastike seosed rabalaama morfomeetria ja veerežiimiga Männik-järve raba näitel", Kristjan Kruusement magistritöö "Fosfori jaotus järvesetetes" ja Peep-Aarne Sirge magistritöö "Eesti endiste sõjakoolide kogemuste rakendamise võimalusi kaitsejõudude tänapäevases väljaõppes"; 21. juunil kaitsevad TPÜ kasvatusteaduste ja pedagoogika magistritööde kaitsmisnõukogu koosolekul Krista Loogma magistritöö "Stsenaariumi meetodi rakendamine hariduse tuleviku uurimisel ja haridusstrateegia arendamisel" ja Tarmo Salumaa magistritöö "Eesmärgistatud organisatsiooniliste muutuste mõju inimeste töökeskkonna tunnetamisele koolis"

Hydrological Modelling the Middle Magdalena Valley (Colombia)

Science.gov (United States)

Arenas, M. C.; Duque, N.; Arboleda, P.; Guadagnini, A.; Riva, M.; Donado-Garzon, L. D.

2017-12-01

Hydrological distributed modeling is key point for a comprehensive assessment of the feedback between the dynamics of the hydrological cycle, climate conditions and land use. Such modeling results are markedly relevant in the fields of water resources management, natural hazards and oil and gas industry. Here, we employ TopModel (TOPography based hydrological MODEL) for the hydrological modeling of an area in the Middle Magdalena Valley (MMV), a tropical basin located in Colombia. This study is located over the intertropical convergence zone and is characterized by special meteorological conditions, with fast water fluxes over the year. It has been subject to significant land use changes, as a result of intense economical activities, i.e., and agriculture, energy and oil & gas production. The model employees a record of 12 years of daily precipitation and evapotranspiration data as inputs. Streamflow data monitored across the same time frame are used for model calibration. The latter is performed by considering data from 2000 to 2008. Model validation then relies on observations from 2009 to 2012. The robustness of our analyses is based on the Nash-Sutcliffe coefficient (values of this metric being 0.62 and 0.53, respectively for model calibration and validation). Our results reveal high water storage capacity in the soil, and a marked subsurface runoff, consistent with the characteristics of the soil types in the regions. A significant influence on runoff response of the basin to topographical factors represented in the model is evidenced. Our calibrated model provides relevant indications about recharge in the region, which is important to quantify the interaction between surface water and groundwater, specially during the dry season, which is more relevant in climate-change and climate-variability scenarios.

Characterization of the vegetation of the park road, Island of Salamanca, Magdalena - Colombia

International Nuclear Information System (INIS)

Tavera Escobar, Hector Arsenio; Gamba Cubides, Nestor Javier

2001-01-01

In Colombia, the natural national parks are par excellence the scenarios to protect the ecological integrity of the ecosystems and consequently for the conservation of the fauna, flora, diversity, genetic resources and values cultural and historical associates. Contrarily, these protected areas are subjected to the degradation caused by anthropic processes and natural that which goes in detriment of the benefits and services that provide to the society. The same as the other protected areas belonging to the system of natural national parks (SNNP), the park road Island of Salamanca (VPIS) it has been subject to the degradation, which has been generated mainly by factors of anthropic character that they have caused an environmental imbalance of great space and temporary magnitude whose repercussion is significant for the fragility of the ecosystems of the protected area in its particular context and for the importance of its function in the region of the complex estuary of the Magdalena River

Reproduction and conservation of the Magdalena River turtle (Podocnemis lewyana) in the Claro Cocorna Sur River, Colombia

International Nuclear Information System (INIS)

Ceballos, Claudia P; Romero, Isabel; Gomez Saldarriaga, Catalina; Miranda, Karla

2014-01-01

The Magdalena river turtle, Podocnemis lewyana, is an endangered and endemic turtle from Colombia. Among the most important information needed to conserve endangered species is to identify, monitor, and protect the sites used by the species to reproduce and grow. In this study we report, for the first time, the reproductive output and the nesting beaches of P. lewyana in the Claro Cocorna Sur River, a tributary of the Magdalena river drainage. We systematically examined a river transect of 8 km with 14 sandy beaches during two nesting seasons in one year. We recorded a yearly production of 47 clutches, 957 eggs, and two preferred nesting beaches: Alto Bonito with 51 %, and Belgica with 28.3 % of this reproductive output. Aafuver, a community-based organization, has led a headstarting program since 2010 to decrease in-situ egg mortality due to predation on nesting beaches. Aafuver collects and incubates the eggs ex-situ, raises the hatchlings for one to five months and then releases them into the same river. To understand potential effects of such egg manipulation, we monitored and compared in-situ and ex-situ incubation temperatures. We found ex-situ temperatures below the pivotal temperature known for P. lewyana and below the temperatures in nesting beaches. Finally, we monitored hatchlings growth under aafuver captive conditions, and found that hatchlings duplicated their body mass during the first three months of age. Egg weight was strongly associated to body weight at hatching; however this association is lost by the third month of age. We strongly encourage supporting this community-based conservation program, and the protection of the Claro Cocorna Sur River as an important nesting and growth habitat for the conservation of P. lewyana.

Preparation and Characterization of Nicke-iron Alloy Film as Freestanding Electrode for Oxygen Evolution Reaction

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Yao Mengqi

2018-01-01

Full Text Available This work reports the porous nicke-iron alloy film supported on stainless steel mesh as freestanding electrode for enhanced oxygen evolution reaction (OER catalyst prepared from an one step electrodeposition method. Results indicated that the porous nickle-iron alloy film exhibits a low overpotential of 270 mV at 10 mA cm-2 and excellent electroconductibility. The superior OER properties can be attributed to its novel synthetic process, conductive substrate and porous structure. This work will provide a new strategy to fabricate alloy film for OER electrocatalyst.

The singer and the song: Nick Cave and the archetypal function of the cover version

OpenAIRE

Wiseman-Trowse, Nathan J B

2013-01-01

Throughout his career, from The Boys Next Door, through The Birthday Party, and with The Bad Seeds, Australian singer / songwriter Nick Cave has balanced his own set of creative voices alongside those of others through his choice of cover versions. Cave’s 1986 album with The Bad Seeds, ‘Kicking Against the Pricks’, is a collection of cover versions that spans American folk idioms (‘Black Betty’, ‘Hey Joe’, ‘The Singer’), Tin-Pan-Alley balladeering (‘Something’s Gotten Hold of my Heart’, ‘By t...

La democratización de los medios de producción: El caso compra de la hacienda La Magdalena

OpenAIRE

Guerra Miño, Darwin Damián

2017-01-01

Analizar el proceso de la democratización de los medios de producción a través del caso de la compra de la hacienda La Magdalena en la parroquia Angochagua, provincia de Imbabura. La Democratización de los medios de producción es una política de estado. La tenencia de la tierra ha sido uno de los principales problemas a solucionar en busca del desarrollo. Este estudio tuvo como objetivo analizar el proceso de la democratización de los medios de producción: tomando como ejemplo el caso d...

Associated fauna to the mangroves and other marshes in the Delta-estuary of the River Magdalena, Colombia

International Nuclear Information System (INIS)

Moreno Bejarano, Luz Myriam; Alvarez Leon, Ricardo

2003-01-01

A synthesis of observations and collections of fauna associated with different vegetative communities and bodies of water present in the estuarine-delta of Magdalena river, with emphasis in the Via Park Isla Salamanca (56.200 acres) and National Reserve Flora and Fauna Sanctuary of the Cienaga Grande de Santa Marta (23.000 acres), between 1978-1985. The information was gathered through samplings and manual captures affected during 1978 and period 1981-1985, as well as accomplished direct observations sporadically in the period 1986-1998. The records of 10 species of amphibians, 40 species of reptiles, 194 species of birds and 46 species of mammals show the great variety of fauna in this Caribbean area of Colombia

Variación diaria de la abundancia del zooplancton en Bahía Magdalena, B.C.S. México Daily cycle of zooplankton abundance in Magdalena Bay, B.C.S. Mexico

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Sergio Hernández-Trujillo

2010-12-01

Full Text Available Se presenta la variación diaria de la abundancia de zooplancton en Bahía Magdalena, México a partir de muestreos en una estación fija durante 10 días en febrero de 2002. De 112 taxa identificados, los nauplios de balanos y los cladóceros Penilia avirostris y Pseudevadne tergestina fueron los más comunes y abundantes y con niveles de variación diaria de más del 100%. Los copépodos constituyeron una quinta parte de la abundancia del zooplancton y 7 de las 80 especies encontradas se registran por vez primera en la zona de estudio. La abundancia del zooplancton fue parcialmente coherente con la hidrografía de la bahía, así como con un modelo de retención de partículas. Las 3 especies de copépodos más abundantes fueron más numerosas a inicio de la serie pero tendieron a reducir su densidad hacia el fin del lapso de estudio. La hidrodinámica invernal en la zona se identificó como un factor de concentración de zooplancton, quizá de importancia mayor que la temperatura o la concentración de clorofila a, aún no estimada en la estructura de la comunidad ni en la intensidad de las relaciones interespecíficas.This paper presents the daily variation of the abundance of zooplankton in Magdalena Bay, Mexico from sampling in a fixed-station for 10 days of February 2002. From 112 taxa identified barnacles nauplii and cladocerans Penilia avirostris and Pseudevadne tergestina were the most common and abundant with daily variation levels more than 100%. The copepods constituted one fifth of zooplankton abundance and 7 of the 80 species found were recorded for the first time in the study area. Zooplankton abundance showed partially coherent pattern with the hidrography of the bay as well as particles retention model. The 3 species of copepods more abundant were more numerous at the beginning of the time series but tended to reduce its density toward the end of the time of study. Winter hydrodynamics was identified as a factor of zooplankton

The worker's massacre of 1928 in the Magdalena Zona Bananera - Colombia. An unfinished story

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Jorge Enrique Elías Caro

2012-01-01

Full Text Available El presente trabajo analiza las condiciones del porqué se presentó la masacre obrera ocurrida el 6 de diciembre de 1928 en la Región Bananera del Magdalena (Colombia por parte de las tropas del Ejército colombiano, para ello, se hace una descripción de las dinámicas del entorno mundial de la fruta, su relación con las compañías multinacionales y el régimen laboral y sindical en Colombia. De igual forma se hace un análisis del contexto y de los antecedentes del conflicto, así como también de la tergiversación de la información presentada por parte del Estado Colombiana y la United Fruit Company, hechos estos que sirvieron de base para inspirar un capítulo de la novela "Cien Años de Soledad" del Nobel en literatura Gabriel García Márquez.

Ambiente, salud y recién nacido: observaciones regionales en el departamento del Magdalena

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Orlando Alarcón Montero

1951-09-01

Full Text Available Este estudio debe comenzar en el siglo XVI en las Antillas, refugio de buscadores de oro y guerreros profesionales que allí esperaron el momento de hacer el aporte europeo a nuestra formación. Otro elemento racial nos llegó en los barcos negros. Y el factor básico estuvo representado por el elemento aborigen, que mostraba grupos situados en diversas estapas de evolución. En el actual Departamento del Magdalena se destacaba la cultura tairona, instalada en las faldas de la Sierra Nevada de Santa Marta. Sus miembros llegaron lejos en el progreso humano. Su habitación era estable, pétrea y de cimiento fuerte. Las poblaciones grandes, de varias centenas de casas, quedan aún desparramadas en forma de ruinas por los valles de la Sierra. Esta solidez de las construcciones traduce una organización económica y social de perfiles definidos.

Aspectos hidro-limnológicos en las ciénagas de Chucuri y Aguas Negras (Magdalena Medio, Colombia) durante un ciclo anual

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García Lozano Luis Carlos; Márquez Calle Germán; Pedraza Gilma Estella

1989-01-01

El presente estudio analiza características hrdrológicas-limnológicas de la estructura y funcionamiento de las ciénagas de Chucuri y Aguas Negras localizadas en la planicie aluvial del Valle Medio del rio Magdalena. Se basa en seguimientos bimensuales en diez estaciones de muestreo, durante el ciclo anual: abril de 1983 a marzo de 1984. Se resalta la presencia de dos estadios definidos asociados al régimen de inundación y sequia con rasgos característicos en la comunidad fitoplanctón...

QUANTIFICATION OF COAL IN THE COLOMBIAN CARIBBEAN BEACHES (DEPARTMENT OF MAGDALENA

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Julián Andrés García

2014-01-01

Full Text Available ABSTRACT To quantify the percentage of unburnt coal in beach sediments from the department of Magdalena,(Colombia a simple procedure was devised to separate the sediment mineral phases according to their density. Sediments were collected from six beaches, at two tidal heights, in November 2012 and February 2013, before and after a spill from a coal barge that occurred on 12 January 2013. Sediments were dried, suspended in an aqueous solution of sodium polytungstate (density: 2.0 g•cm-3 and centrifuged. Because coal has a lower density (1.2-1.9 g•cm-3 than other inorganic minerals and rocks, any coal particle present in a sample concentrated in the suspended fraction, whereas more dense particles settled. To remove organic matter not corresponding to coal, the light fraction was subsequently subjected to a weak oxidation with hydrogen peroxide. To estimate an upper limit for any coal present, weight loss on ignition at 550 °C was determined for whole sediments. For the six beaches examined, the maximum weight loss on ignition was 2.4 % and the weight percentage of the light fraction (with a density 3 g•cm-3, which comprise between 10-70 % of the total sediment dry weight. RESUMEN Con el fin de cuantificar el porcentaje de carbón mineral en playas del departamento del Magdalena, (Colombia se llevó a cabo un procedimiento sencillo para separar el sedimento en fracciones sedimentológicas de acuerdo a su densidad. Se utilizaron sedimentos provenientes de seis playas del departamento colectadas de dos niveles mareales en noviembre de 2012 y febrero de 2013, antes y después de un derrame de una barcaza de carbón, que ocurrió el 12 de enero de 2013. Los sedimentos fueron secados, suspendidos en una solución de sodio politungstato (densidad: 2,0 g·cm-3 y centrifugados. Ya que el carbón tiene una densidad menor (1,2-1,9 g·cm-3 que otros minerales inorgánicos, cualquier partícula de carbón presente en una muestra se concentró en la

Petrotectonic characteristics, geochemistry, and U-Pb geochronology of Jurassic plutons in the Upper Magdalena Valley-Colombia: Implications on the evolution of magmatic arcs in the NW Andes

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Rodríguez, G.; Arango, M. I.; Zapata, G.; Bermúdez, J. G.

2018-01-01

Field, petrographic, and geochemical characterization along with U-Pb zircon geochronology of the Jurassic plutons exposed in the Upper Magdalena Valley (Colombia) allowed recognizing distinct western and eastern suites formed in at least three magmatic pulses. The western plutons crop out between the eastern flank of the Central Cordillera and the Las Minas range, being limited by the Avirama and the Betania-El Agrado faults. The western suite comprises a quartz monzonite - quartz monzodiorite - quartz diorite series and subordinate monzogranites. Chemically, the rocks are high-K calc-alkaline I-type granitoids (some reaching the shoshonitic series) with metaluminous of magnesium affinity. Trace-element tectonic discrimination is consistent with magmatism in a continental arc environment. Most rocks of this suite crystallized between 195 and 186 Ma (Early Jurassic, Pliensbachian), but locally some plutons yielded younger ages between 182 and 179 Ma (Early Jurassic, Toarcian). The eastern suite crops out in the eastern margin of the Upper Magdalena Valley, east of the Betania - El Agrado fault. Plutons of this unit belong to the monzogranite series with rock types ranging between syenogranites and granodiorites. They are high-K calc-alkaline continental granitoids, some metaluminous and some peraluminous, related to I-type granites generated in a volcanic arc. Crystallization of the suite was between 173 and 169 Ma (Middle Jurassic, Aalenian-Bajocian), but locally these rocks contain zircon with earlier inherited ages related to the magmatic pulse of the western suite between 182 and 179 Ma (Early Jurassic, Toarcian). The evolution of the Jurassic plutons in the Upper Magdalena Valley is best explained by onset or increase in subduction erosion of the accretionary prism. This explains the eastward migration of the arc away from the trench. Subduction of prism sediments increased the water flux from the subducting slab, decreasing solidus temperatures, therefore

Presencia del maltrato y abuso sexual en el hogar de estudiantes de secundaria en tres colegios públicos de la ciudad de Santa Marta – Magdalena- (Colombia

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Yuris Maria Batista Vargas

2013-11-01

Full Text Available Title: Presence of abuse and sexual abuse in the home of high school students in three public schools of the city of Santa Marta - Magdalena-(Colombia.ResumenEl presente estudio se fundamentó en identificar la presencia del maltrato infantil y abuso sexual en el hogar de estudiantes adolescentes, matriculados para el año 2011 en tres colegios públicos de estrato uno de la ciudad de Santa Marta – Magdalena. La muestra estuvo conformada por 230 participantes, en edades entre 14 y 16 años, quienes fueron evaluados a través del ISPCAN, versión para niños/as (ICAST-C. Los resultados señalan la existencia del maltrato físico (13%, psicológico (29% y de abuso sexual (32%. Por otra parte, se encontró que el género femenino obtuvo mayor porcentaje en el maltrato de tipo físico y sexual, mientras que los varones presentan mayor  porcentaje de maltrato psicológico. Se concluye que uno de cada tres estudiantes ha sido objeto de los tipos de abuso en estudio. (DUAZARY 2013 No. 1, 67 - 72AbstractThis study was based on identifying the presence of child abuse and sexual abuse in the home of teenage students, enrolled for 2011 in three public schools in tier one of the city of Santa Marta - Magdalena. The sample consisted of 230 participants, aged between 14 and 16 years, who were evaluated through ISPCAN, children’s version / as (ICAST-C. The results indicate the existence of physical abuse (13%, psychological (29% and sexual abuse (32%. Moreover, it was found that female scored higher percentage of physical abuse and sexual abuse, while males have a higher rate of psychological abuse. It is concluded that one in three students has been the subject of study types of abuse.Keywords: violence; sexual violence; university

Formas de carbono orgánico en suelos con diferentes usos en el departamento del Magdalena (Colombia Some forms of organic carbon in soil with different uses in the Department of Magdalena (Colombia

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José Rafael Vásquez-Polo

2011-12-01

Full Text Available Las fracciones de materia orgánica del suelo (MOS lábiles y humificadas pueden ser afectadas por las prácticas de uso y manejo; sin embargo el impacto de estos cambios no se ha evaluado en suelos y ambientes tropicales. El presente estudio tuvo como objetivo evaluar los contenidos y algunas formas de carbono orgánico del suelo (COS en cinco zonas de clima cálido tropical (0 - 1110 m.s.n.m. del departamento del Magdalena (Colombia y el efecto que sobre ellas han tenido las prácticas asociadas a suelos cultivados con café (Coffea arabica, banano (Musa sp., palma africana (Elaeis guineensis y sábila (Aloe vera, comparados con suelos de bosques naturales. No se encontraron diferencias (P Fractions of soil organic matter (SOM labile and humified, can be affected by use and management practices, but the impact of these changes has not been evaluated in soils of tropical environments. The present study investigated the contents and some forms of soil organic carbon (SOC in five warm tropical climate zones of the Department of Magdalena (Colombia, and the effect of the cropping practices on these forms of organic carbon in cultivated soils, associated with Coffee (Coffea arabica, Banana (Musa sp., African palm (Elaeis guineensis, Aloe (Aloe vera compared to natural forest soils. Significant differences (P < 0.05 were not found between zones as much use soil as. Low average values of SOM in the study areas and higher contents of total carbon in forest soils than in cultivated soils were reported. Forest soils had an average carbon accumulation total of 42.4 mg/ha at 20 cm, compared to 33.8 mg/ha in the cultivated soils, this equates to an average loss of 23% total C by the effect of crop management in these soils, compared to C humified (C extracted with sodium pyrophosphate, values are observed very low in cultivated soils and almost zero in forest soils, but forest soils had a higher number of stable forms of C (Cnox. In the soil cultivated

SimBasin: serious gaming for integrated decision-making in the Magdalena-Cauca basin

Science.gov (United States)

Craven, Joanne; Angarita, Hector; Corzo, Gerald

2016-04-01

The Magdalena-Cauca macrobasin covers 24% of the land area of Colombia, and provides more than half of the country's economic potential. The basin is also home a large proportion of Colombia's biodiversity. These conflicting demands have led to problems in the basin, including a dramatic fall in fish populations, additional flooding (such as the severe nationwide floods caused by the La Niña phenomenon in 2011), and habitat loss. It is generally believed that the solution to these conflicts is to manage the basin in a more integrated way, and bridge the gaps between decision-makers in different sectors and scientists. To this end, inter-ministerial agreements are being formulated and a decision support system is being developed by The Nature Conservancy Colombia. To engage stakeholders in this process SimBasin, a "serious game", has been developed. It is intended to act as a catalyst for bringing stakeholders together, an illustration of the uncertainties, relationships and feedbacks in the basin, and an accessible introduction to modelling and decision support for non-experts. During the game, groups of participants are led through a 30 year future development of the basin, during which they take decisions about the development of the basin and see the impacts on four different sectors: agriculture, hydropower, flood risk, and environment. These impacts are displayed through seven indicators, which players should try to maintain above critical thresholds. To communicate the effects of uncertainty and climate variability, players see the actual value of the indicator and also a band of possible values, so they can see if their decisions have actually reduced risk or if they just "got lucky". The game works as a layer on top of a WEAP water resources model of the basin, adapted from a basin-wide model already created, so the fictional game basin is conceptually similar to the Magdalena-Cauca basin. The game is freely available online, and new applications are being

Mompox y el río grande de la Magdalena: Patrimonio cultural y natural de colombia y la humanidad

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Franco Ossa Lucía Victoria

2011-11-01

Full Text Available El sistema estructurante urbano­regional de Mompox esta configurado por su arquitectura y el río Grande de la Magdalena, es decir, por su patrimonio cultural y ambiental. A su vez, Mompox constituye un hito estructurante del desarrollo regional, en el contexto histórico, urbano­ arquitectónico, ambiental y socio­cultural, y un referente importante de la memoria de la humanidad, en tanto está inscrito en la lista del patrimonio mundial desde 1995. La conservación, tutela y revitalización de estos hitos culturales y naturales dependen de estrategias sistémicas de valoración, planeamiento, gestión y educación de las valías patrim oniales culturales y ambientales de Mompox y la región. El objetivo de esta publicación es poner en valor el patrimonio de Mompox y del río Grande de la Magdalena, como ejes estructurantes de fundación, poblamiento y desarrollo urbano, y como marcas del paisaje y ejes de multiculturalidad y biodiversidad regional, a partir de la valoración de su historia, arquitectura y ambiente y la concepción del patrimonio com o sistema contenedor de la expresión humana en su íntima relación con el entorno. El patrimonio de Mompox es tratado desde su contexto histórico durante los períodos pre­hispánico, colonial y el más reciente el aprovechamiento de los recursos naturales y la actividad fluvial en los procesos de poblamiento y colonización española y la configuración urbana de la ciudad.

La masacre obrera de 1928 en la zona bananera del Magdalena-Colombia. Una historia inconclusa

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Jorge Enrique El\\u00EDas Caro

2011-01-01

Full Text Available El presente trabajo analiza las condiciones del porqué se presentó la masacre obrera ocurrida el 6 de diciembre de 1928 en la Región Bananera del Magdalena (Colombia por parte de las tropas del Ejército colombiano, para ello, se hace una descripción de las dinámicas del entorno mundial de la fruta, su relación con las compañías multinacionales y el régimen laboral y sindical en Colombia. De igual forma se hace un análisis del contexto y de los antecedentes del conflicto, así cómo también de la tergiversación de la información presentada por parte del Estado Colombiana y la United Fruit Company, hechos estos que sirvieron de base para inspirar una parte de la novela Cien Años de Soledad del Nóbel en literatura Gabriel García Márquez.

Paleopatologías dentales más frecuentes en restos óseos de habitantes hallados en el Complejo Arqueológico El Brujo - Magdalena de Cao

OpenAIRE

Chanamé Marín, Ann Rosemary

2013-01-01

The objective of the study was to determine the frequency of dental paleopathologies inhabitants skeletal remains found in the archaeological complex El Brujo - Magdalena de Cao. The population consisted of 106 skeletal remains over 18 years, according to the inclusion criteria. We evaluated dental caries, malocclusion, dental crowding, address anomalies, anomalies site, age and gender, using a data collection instrument and which reached the following results: . This study allows us to prese...

PESCADORES DE LOS RAUDALES DEL RÍO MAGDALENA DURANTE EL PERIODO FORMATIVO TARDÍO

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Peña German

2011-12-01

Full Text Available Los resultados de la investigación realizada en el yacimiento arqueológico de Arrancaplumas, localizado en la zona de raudales del río Magdalena, entre la cuenca alta y media (Honda - Tolima y Puerto Bogotá - Cundinamarca, indican que este lugar fue habitado por largas temporadas anuales entre los siglos V y I A.C. Los pobladores emplearon en sus actividades cotidianas a lo largo de toda la ocupación una misma clase de cerámica y elaboraron una industria lítica que no presenta cambios significativos a través del tiempo.  El predominio de líticos lascados probablemente están relacionados con actividades derivadas de la pesca y la caza. La producción de alimentos vegetales se redujo principalmente al cultivo esporádico de maíz y papaya, y la recolección de frutos de palma. Durante toda la secuencia de ocupación la pesca constituyó la principal actividad de la cual derivaron su subsistencia.

EVALUACIÓN DE LA ROTIFEROFAUNA PRESENTE EN EL COMPLEJO DE PAJARALES DURANTE LA ÉPOCA LLUVIOSA, DEPARTAMENTO DE MAGDALENA, COLOMBIA

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SEBASTIÁN CELIS

2008-05-01

Full Text Available Con miras a evaluar posibles efectos de la recomunicación del río Magdalena con su antiguo delta, se determinó la abundancia y composición de la rotiferofauna del Complejo de Pajarales (CP, durante el período de mayor abundancia anual de zooplancton. El phylum Rotifera fue seleccionado por sus altas tasas reproductivas y eficiencia en procesos de transformación energética. Las muestras se colectaron con botella van Dorn durante la segunda temporada lluviosa de 2006. En cada una de las cuatro estaciones analizadas se registraron la salinidad, temperatura, pH y oxígeno disuelto. La diversidad se calculó empleando el índice de Shannon-Wiener H’ (log10 comparando estaciones y fechas de muestreo para establecer variaciones en el período de estudio. Gráficamente se relacionaron variables fisicoquímicas con valores de diversidad que emplearon un α de 0.05 y 95% de intervalo de confianza. En total, fueron encontrados 20 morfotipos pertenecientes a las familias Brachionidae, Lecanidae, Filiniidae, Synchaetidae, Hexarthriidae y Testudinellidae, siendo Brachionidae y Lecanidae las más abundantes. La relación entre diversidad y variables fisicoquímicas, indica que la salinidad es la principal responsable de la diversidad de rotíferos. En conclusión, la rotiferofauna actual en el CP es más abundante y diversa que hace 16 años, antes de la recomunicación con el río Magdalena. Así mismo, la concentración de oxigeno disuelto, pH y salinidad son diferentes. Teniendo en cuenta que la salinidad es el factor que más influyó en la diversidad de rotíferos, la reapertura de canales probablemente favoreció el incremento en la diversidad de rotíferos en el CP.

REPTILES DEL VALLE SECO DEL RÍO MAGDALENA (HUILA, COLOMBIA

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RAFAEL MORENO-ARIAS

2015-06-01

Full Text Available Se presenta una caracterización de la fauna de reptiles del valle seco del río Magdalena en el departamento del Huila, así como la comparación faunística entre unidades de cobertura vegetal y épocas climáticas. Se registraron 31 especies pertenecientes a 30 géneros, 17 familias y dos órdenes. En el orden Squamata, la familia más diversa fue Teiidae (lagartijas con tres especies y Colubridae (serpientes con nueve. Para el orden Testudines se registró una especie. Con base en curvas de acumulación de especies y los estimadores no paramétricos Jackknife 2 y Bootstrap, para las lagartijas se obtuvo una alta representatividad en el muestreo (83% y 92% respectivamente, mientras que para las serpientes fue menor (75% y 82% respectivamente. La estructura y composición de los ensambles de reptiles en cada unidad de cobertura vegetal no fue significativamente diferente, en general se caracterizaron por presentar pocas especies con muchos individuos y numerosas especies raras. El arbustal presentó la mayor riqueza de especies seguido del bosque de ribera y los cultivos de cacao. En general la abundancia de reptiles fue mayor en la época de lluvias que en la época seca, sin embargo la manera en que responden las especies a las épocas climáticas está dada por sus características ecológicas, fisiológicas y comportamentales.

El papel de los manglares en la producción de las comunidades acuáticas de Bahía Magdalena, B.C.S.

OpenAIRE

Chávez Rosales, Samuel

2006-01-01

El bosque de manglar de Bahía Magdalena se ubica entre dos ecosistemas ca características totalmente diferentes, por lo que constituyen en si una zona d transición, en donde se presenta una gran complejidad funcional y estructural. S establecieron las características de cobertura, productividad y estructura del bosque, así como la transferencia de energía del manglar, a la zona acuática adyacente mediante un modelo de flujos tróficos. Para el caso se consideró que la productividad inducida po...

Exploraciones arqueológicas en la Costa Atlántica y Valle del Magdalena: Sitios Precerámicos y Tipologías Líticas

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Correal Urrego Gonzalo

1977-06-01

Full Text Available El presente trabajo corresponde a una síntesis de los sitios Precerámicos descritos en el estudio "Exploraciones Arqueológicas en la Costa Atlántica y Valle del Magdalena", estudio en el cual son presentados en extenso (cinco tomos, junto con los hallazgos de industrias líticas, los resultados obtenidos en nuevos sitios cerámicos y arte rupestre en el país, junto con los datos geográficos, geológicos y etnohistóricos de las áreas investigadas.

Primera, área protegida, en el partido de Magdalena para la preservación del Ombusillo (Phytolacca tetrámera Hauman,in situ.

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Inés Petri

2010-07-01

Full Text Available Acuerdo entre la Dirección de Vialidad de la Provincia de Buenos Aires y la Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata,Argentina. En el decanato de la Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata, se firmo un convenio para la creación de un área para la investigación y desarrollo de conocimiento en defensa de Phytolacca tetrámera Hauman (ombucillo, en las canteras de conchilla que la Dirección de Vialidad de la Provincia de Buenos Aires posee en la Ruta Provincial nº 11, Km. 37,5 del Partido de Magdalena. Se genera así un área para la conservación in situ (en su lugar de origen de esta especie endémica (la cual no se la encuentra en estado natural en otro lugar del mundo del SE de la Provincia de Buenos Aires, que se halla en riesgo critico de extinción, con un área de distribución muy restringida, a las puertas de las zonas más pobladas del país. Actualmente se encuentran poblaciones o individuos aislados de esta especie mayoritariamente en los partidos de Magdalena y Punta Indio y sus alrededores. Dado su parentesco con otras especies del género Phytolacca, el ombú entre otras, presenta propiedades fungicidas y fungí estáticas y podría contener compuestos antitumorales, antivirales, bactericidas e insecticidas.

Aplicación de modelo ARIMA para el análisis de series de volúmenes anuales en el río Magdalena

OpenAIRE

Amaris, Gloria; Ávila, Humberto; Guerrero, Thomas

2017-01-01

Resumen Contexto: Los efectos del cambio climático, intervenciones humanas y características de los ríos, son factores que incrementan el riesgo en la población y de los recursos hídricos. Sin embargo, impactos negativos como inundaciones y desecación de ríos pueden ser identificados previamente mediante el uso de herramientas de modelación adecuadas. Objetivos: Se estima un modelo ARIMA para el análisis de series de tiempo de volúmenes anuales (millones de m³/año) en el río Magdalena usando ...

W. Horsley Gantt, Nick, and the Pavlovian Science at Phipps Clinic.

Science.gov (United States)

Ruiz, Gabriel; Sánchez, Natividad

2016-10-24

William Horsley Gantt is well known as one to the principal proponents of Pavlovian methodology in the U.S. After a long stay at Ivan Petrovich Pavlov's laboratory in Leningrad from 1925 to 1929, Gantt was invited by Adolf Meyer to join the Henry Phipps Psychiatric Clinic, where he founded and directed the Pavlovian Laboratory from 1930 to 1964. Soon after his arrival at Phipps Clinic in 1931, Gantt began a Pavlovian research program that included the investigation of nervous disturbances in dogs and clinical researches with psychiatric patients. In these studies, Gantt combined a physiological method (the conditional reflexes approach), with a psychiatric problem (nervous disorders) in the context of Meyer's psychobiology that established the person or individual as unit of analysis. This fact, concentrating upon a single individual, made Gantt studies with dogs recognizable and interesting to physicians, psychologists, and psychiatrists who also worked on individuals. In this paper, we use archival materials -including correspondence, notebooks, and unpublished autobiographical material- to present a case study, that of William Horsley Gantt and his dog Nick. We will explore the reasons why Gantt' studies on nervous disturbances with this dog captured the interest of psychiatrists and clinical psychologists.

On the use of an appropriate TdT-mediated dUTP-biotin nick end labeling assay to identify apoptotic cells.

OpenAIRE

Lebon, C.; Rodriguez, G.V.; Zaoui, I.E.; Jaadane, I.; Behar-Cohen, F.; Torriglia, A.

2015-01-01

Apoptosis is an essential cellular mechanism involved in many processes such as embryogenesis, metamorphosis, and tissue homeostasis. DNA fragmentation is one of the key markers of this form of cell death. DNA fragmentation is executed by endogenous endonucleases such as caspase-activated DNase (CAD) in caspase-dependent apoptosis. The TUNEL (TdT-mediated dUTP-biotin nick end labeling) technique is the most widely used method to identify apoptotic cells in a tissue or culture and to assess dr...

Benthic biodiversity and ecological gradients in the Seno Magdalena (Puyuhuapi Fjord, Chile)

Science.gov (United States)

Betti, F.; Bavestrello, G.; Bo, M.; Enrichetti, F.; Loi, A.; Wanderlingh, A.; Pérez-Santos, I.; Daneri, G.

2017-11-01

Due to its complex hydrological, geomorphological and climatic features, the Chilean fjords region is considered among the most productive areas of the world. The benthic fauna of this region accounts for more than 1600 species showing marked latitudinal biogeographic differences characterizing this as one of the most important hotspot of biodiversity of cold-temperate environments. Despite numerous studies have been conducted to depict the biological characteristics of the fjords, the present situation is strongly unbalanced towards specific taxa. Hence, this study takes into consideration a community approach, highlighting the distribution of six benthic assemblages thriving on vertical walls along the Seno Magdalena fjord (Aysen region). Underwater pictures were used to characterize the trends in abundance and diversity of the main taxa showing distinct responses to salinity and turbidity. Among the less tolerant taxa to high fresh water inputs there are encrusting algae, mainly found in the most external sites lashed by outer currents, far from the estuarine plume. The bathymetric zonation of the assemblages, instead, is characterized by a dense mussel belt in the first 10 m, within a thick layer of low-salinity, nutrient-enriched waters. Rich assemblages of sponges, brachiopods, gorgonians and scleractinians thrive in deeper, marine, clear waters. The evaluation of the ecological role of benthic species leads both to the definition of potential bioindicator taxa responding to anthropic disturbances and to the promotion of protected areas.

Leveraging Client-Side DNS Failure Patterns to Identify Malicious Behaviors

Science.gov (United States)

2015-09-28

malicious behavior found in our dataset and (ii) to create ground truth to evaluate the system proposed in Section V. We begin by removing those cases that...2011. [10] S. Hao, N. Feamster, and R. Pandrangi, “Monitoring the Initial DNS Behavior of Malicious Domains,” in ACM IMC , 2011. [11] R. Perdisci et...distribution is unlimited. Leveraging Client-Side DNS Failure Patterns to Identify Malicious Behaviors The views, opinions and/or findings contained in

Interacciones de pesquerías ribereñas en Bahía Magdalena-Almejas, Baja California Sur

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Miguel A. Ojeda Ruiz de la Peña

2012-01-01

Full Text Available Con el fin de aportar al desarrollo de un modelo conceptual sobre la pesca ribereña o artesanal en el área de Bahía Magdalena-Almejas, Baja California Sur, se jerarquizó la importancia de 14 pesquerías de la región. La calificación se basó en un análisis cualitativo por puntos y en la estimación de un índice de importancia relativa, con indicadores como los promedios de captura, valor y frecuencia de registro de especies objetivo en desembarcos de embarcaciones menores, de 1998 a 2009; entre ellas están: almeja catarina, escama, camarón, callo de hacha, tiburón, jaiba, calamar, almeja pata de mula, rayas, lisa, pulpo, abulón, langosta y caracol chino. La interacción temporal entre varias de ellas es controlada por vedas oficiales, pero cuando ocurre se reconocen posibles efectos por pesca incidental e ilegal y modificaciones al ecosistema.

Estructura de mercado en la comercialización del banano tipo exportación producido en la Zona Bananera del Magdalena y comportamiento estratégico de las empresas

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Janny Acuña Fuentes

2014-01-01

Full Text Available La estructura de mercado en la comercialización del banano tipo exportación producido en el departamento del Magdalena históricamente ha sido imperfecta, siendo las comercializadoras multinacionales quienes fijan las cantidades de banano que compran a los productores, o el precio, generando una pérdida de bienestar social a los productores de banano. En la presente investigación se midió el grado de concentración de mercado por parte de las comercializadoras a través del índice de concentración Herfindahl-Hirschman (IHH; posteriormente, a través de econometría de series de tiempo, se procedió a mostrar la relación entre los precios pagados a los productores y el IHH, por último, mediante el modelo de Cournot y de Stackelberg, a partir de la estimación econométrica de una función inversa de oferta y la aplicación de estrategias puras en juegos no cooperativos, se probó empíricamente las estrategias que las comercializadoras están llevando a cabo.Palabras Clave: Cournot; Stackelberg; Bertrand; Equilibrio de Nash; índice Herfindahl-Hirschman; teoría de juegos Market structure in the marketing of bananas export type produced in the area of Magdalena and strategic business behaviorAbstractThe market structure in the marketing of banana export type produced in the department of Magdalena historically has been imperfect, with multinational marketers who will determine the quantities of bananas they buy from the producers or the price, generating a loss of social welfare to banana producers. In the present study measured the degree of market concentration by marketers across the Herfindahl concentration index-Heirschman (HHI, then through econometric time series proceeded to show the relationship between the prices paid to producers and the concentration index (HHI, finally using the model of Cournot and Stackelberg, from the econometric estimation of an inverse function of supply and application of pure strategies in

Formas de hierro y aluminio en suelos con diferentes usos en la zona norte del departamento del Magdalena, Colombia

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José Rafael Vásquez Polo

2014-12-01

Full Text Available En el estudio se evaluaron las diferentes formas de hierro (Fe y aluminio (Al presentes en suelos cultivados y en áreas de bosque de la zona norte del departamento del Magdalena (Colombia. Se seleccionaron seis zonas de muestreo localizadas entre 0 y 1000 m.s.n.m. Se utilizaron tres técnicas selectivas de extracción de Fe y Al con los agentes extractantes: pirofosfato de sodio, oxalato ácido de amonio, y ditionito citrato bicarbonato. Los contenidos totales de Fe y Al fueron determinados mediante un ataque ácido y cuantificación por absorción atómica. El análisis de varianza multivariado mostró diferencias significativas (P 3% del Al total, lo que sugiere que un alto contenido de Al forma parte de estructuras cristalinas.

Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats.

Science.gov (United States)

Gabsalilow, Lilia; Schierling, Benno; Friedhoff, Peter; Pingoud, Alfred; Wende, Wolfgang

2013-04-01

Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions or deletions. The error-prone NHEJ can be efficiently suppressed by 'nickases' that produce a single-strand break rather than a double-strand break. Highly specific nickases have been produced by engineering of homing endonucleases and more recently by modifying zinc finger nucleases (ZFNs) composed of a zinc finger array and the catalytic domain of the restriction endonuclease FokI. These ZF-nickases work as heterodimers in which one subunit has a catalytically inactive FokI domain. We present two different approaches to engineer highly specific nickases; both rely on the sequence-specific nicking activity of the DNA mismatch repair endonuclease MutH which we fused to a DNA-binding module, either a catalytically inactive variant of the homing endonuclease I-SceI or the DNA-binding domain of the TALE protein AvrBs4. The fusion proteins nick strand specifically a bipartite recognition sequence consisting of the MutH and the I-SceI or TALE recognition sequences, respectively, with a more than 1000-fold preference over a stand-alone MutH site. TALE-MutH is a programmable nickase.

Repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6, and rad9 of Saccharomyces cerevisiae. [nicking

Energy Technology Data Exchange (ETDEWEB)

Prakash, L [Rochester Univ., N.Y. (USA). Dept. of Radiation Biology and Biophysics; Rochester Univ., N.Y. (USA). School of Medicine and Dentistry)

1977-10-01

The ability to remove ultraviolet-induced pyrimidine dimers was examined in four radiation-sensitive mutants of Saccharomyces cerevisiae. The susceptibility of DNA from irradiated cells to nicking by either the T4 uv-endonuclease or an endonuclease activity found in crude extracts of Micrococcus luteus was used to measure the presence of dimers in DNA. The rad3 and rad4 mutants are shown to be defective in dimer excision whereas the rad6 and rad9 mutants are proficient in dimer excision.

Estructura de la comunidad y asociación de las aves acuáticas con la heterogeneidad espacial del complejo lagunar Bahía Magdalena-Almejas, Baja California Sur, México

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Bulmara Zárate-Ovando

2008-03-01

Full Text Available Para probar la hipótesis que la heterogeneidad espacial determina la diversidad de aves acuáticas en un humedal costero, comparamos la densidad, diversidad y composición de especies de aves acuáticas entre habitats y otras unidades de paisaje del complejo Bahía Magdalena-Almejas, Baja California Sur, México. Se realizaron censos de aves acuáticas en 329 segmentos de habitat de la línea de costa interna del complejo lagunar, desde febrero 2002 hasta febrero 2003. La densidad de aves fue mayor en invierno y otoño en las tres lagunas del área de estudio; de las zonas, la densidad fue mayor en la costa insular; y de los habitats en playa arenosa y dunas pero en los pocos segmentos de sustrato antrópico la densidad fue alta. La diversidad fue significativamente mayor en otoño; en Canal Santo Domingo; en la costa peninsular; y de los habitats en manglar-dunas y manglar. La modificación del habitat natural costero y el disturbio por tránsito de embarcaciones no tuvieron influencia en la densidad o diversidad de aves acuáticas, pero la riqueza acumulada mayor ocurrió en las costas bien conservadas y sin disturbio. El análisis de la composición de especies mostró diferencias entre las asociaciones de Canal Santo Domingo y Bahía Magdalena. El ambiente pelágico y el manglar tuvieron diferencia mayor en la composición de especies con respecto a las asociaciones de los demás habitats, tales diferencias en elencos de especies por habitat sugieren que la comunidad de aves está estructurada de acuerdo a los recursos naturales disponibles de las lagunas, zonas y habitats, en relación con la complejidad estructural de las unidades de paisaje. Las condiciones ecológicas del complejo lagunar ocasionan que las aves acuáticas piscívoras sean el grupo dominante de esta comunidad.Community structure and association of waterbirds with spatial heterogeneity in the Bahía Magdalena-Almejas wetland complex, Baja California Sur, Mexico. To test

Parámetros genéticos para características reproductivas en una población de búfalos (bubalus bubalis artiodactyla, bovidae) en el magdalena medio colombiano.

OpenAIRE

Bolívar Vergara, Diana María; Ramírez Toro, Edison Julián; Agudelo Gómez, Divier Antonio; Angulo Arroyave, Roberto Arturo; Cerón Muñoz, Mario Fernando

2011-01-01

La investigación se hizo para estimar los componentes de (co)varianza y parámetros genéticos para edad al primer parto (EPP) e intervalo entre partos (IEP) en una población bufalina en el Magdalena Medio Colombiano. Las características evaluadas fueron EPP, IEP entre primer y segundo parto (IEP1-2), IEP entre segundo y tercer parto (IEP2-3), e IEP entre tercer y cuarto parto (IEP3-4) en un modelo animal multicaracterístico. El IEP con todos los partos (IEPtotal) fue analizado en un modelo ani...

Magdalena Ridge Observatory Interferometer -- First Light with Telescope 1 and Progress to Fringes

Science.gov (United States)

Creech-Eakman, Michelle J.; Haniff, Chris A.; Buscher, David F.; Young, John S.; Payne, Ifan; Romero, Van D.; Magdalena Ridge Observatory Interferometer Team

2018-01-01

The Magdalena Ridge Observatory Interferometer (MROI), a 10-telescope optical/near-IR interferometer in central NM has been conceived to be the most ambitious optical interferometric array under construction to date. With baselines ranging from 7.8 to 343 meters, and limiting magnitudes of 14 at K band, it will be able to assess many thousands of astronomical targets on spatial scales of 10's to 0.1's of milliarcseconds. After over a decade of funding from NRL and the major partner institutions (NM Tech and Cambridge University), new funding was obtained in late 2015 via a Cooperative Agreement between NM Tech and the Air Force Research Lab (AFRL) to bring the facility to a three-interferometer system capable of observing geosynchronous satellites. However, we still maintain an exciting and compelling astronomical portfolio which will produce statistical samples of: YSOs and their surrounding disks, systems dominated by mass-loss and mass-transfer, pulsational stars and binary systems, and image the environs of AGN in nearby galaxies. An update on the status and plans for MROI for the next 3 years under the current Cooperative Agreement will be presented. In addition, we will present some examples of observational applications feasible with MROI both in the near-term and as we approach the full 10-telescope facility and describe how the astronomical community can become involved in this exciting project.We wish to acknowledge our sponsors in the NM Congressional Delegation and AFRL FA #9453-15-2-0086 for our recent support.

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

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Takuya Sato

2015-07-01

Full Text Available Mouse spermatogonial stem cells (SSCs can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS cells, are targets of genome modification, this process remains technically difficult. In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. The Rosa26-targeted GS cells differentiated into fertility-competent sperm following transplantation. On the other hand, Stra8-targeted GS cells showed defective spermatogenesis following transplantation, confirming its prime role in the initiation of meiosis. TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure.

APORTES DE LA PSICOLOG ÍA JURÍDICA A LOS PROCESOS DE ACOMPAÑAMIENTO PSICOSOCIAL A LAS VICTIMAS DE LA VIOLENCIA EN EL DEPARTAMENTO DEL MAGDALENA

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Cindy Tobías Loaiza

2011-01-01

Full Text Available Las víctimas de la violencia reciben apoyo por parte del Estado para obtener acompañamiento psicosocial y reparar el daño sufrido. A pesar de los innegables avances normativos materializados en el marco de la ley de víctimas, estas corren el riesgo de ser revictimizadas. El presente informe de investigación recurre a metodologías cualitativas y a la psicología jurídica para determinar las mejores prácticas en la orientación de los procesos de acompañamiento psicosocial a víctimas de la violencia. La población objeto de estudio se encuentra en el Departamento del Magdalena.

Processive nicking activity of T4 endonuclease V on UV-irradiated chromatin

International Nuclear Information System (INIS)

Gruskin, E.A.; Lloyd, R.S.

1986-01-01

T4 endonuclease V initiates the excision repair of pyrimidine dimers in UV-irradiated T4 infected E. coli cells. The pyrimidine dimer specific nicking activity of T4 endonuclease V functions by a processive scanning on UV-irradiated DNA. Previously it has been demonstrated that introduction of endonuclease V into repair-deficient human cells causes a restoration of UV survival in these cells. This demonstrates that endonuclease V is competent to incise mammalian DNA at the site of pyrimidine dimers. In order to assess the ability of endonuclease V to act processively on DNA associated as chromatin, minichromosomes were prepared for use as a substrate. Form I DNA was reconstituted with H3, H4 +/- H1 histones by sequential dialysis steps from 2.0 M NaCl to 50 mM NaCl. Time course reactions were performed with minichromosomes containing 10 and 25 dimers per molecule. In each case the rate of disappearance of form I DNA which was associated as chromatin was decreased relative to that of naked form I DNA. Concurrent with that observation, the rate and extent of appearance of form III DNA was increased with the DNA in minichromosomes relative to naked DNA. This is diagnostic of an enhancement of processivity. The inclusion of H1 in the minichromosomes resulted in a slight additional increase in processivity relative to minichromosomes consisting only of H3 and H4

MEMORIA Y RESILIENCIA. Estudio de la memoria de las victimas del conflicto armado en el departamento del magdalena: presentificacion, visibilizacion, catarsis y resiliencia

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Edimer Leonardo Latorre Iglesias

2010-01-01

Full Text Available Las víctimas de la violencia en el Departamento del Magdalena han sido silenciadas por el olvido sistemático del gobierno, de la sociedad en general y de los medios masivos de comunicación, que al establecer sus agendas de contenidos, no tienen en cuenta el sufrimiento casi permanente de los tradicionalmente excluidos de la sociedad. En ese proceso de negación constante y de aplazamiento de los derechos de las personas en condición de desplazados forzosos, las víctimas de la violencia desarrollan interna y externamente resiliencia. El presente artículo, retoma de forma general los aspectos claves de la investigación, sobre memoria y resiliencia que actualmente desarrolla la Escuela de Comunicación social y periodismo de la Universidad Sergio Arboleda seccional Santa Marta.

VISIBILIZACIÓN DE LA MEMORIA DE LAS VÍCTIMAS DE LA VIOLENCIA EN EL DEPARTAMENTO DEL MAGDALENA: RESILIENCIA PARA CONSTRUIR VERDAD JURIDICA

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Edimer Leonardo Latorre Iglesias

2011-01-01

Full Text Available Las víctimas de la violencia en el Departamento del Magdalena han sido silenciadas por el olvido sistemático del gobierno, de la sociedad en general y, de los medios masivos de comunicación; ya que establecen sus agendas de contenidos sin tener en cuenta el sufrimiento, casi permanente, de quienes han sido tradicionalmente excluidos de la sociedad. En ese proceso de negación constante y de aplazamiento de los derechos de las personas en condición de desplazados forzosos, las víctimas de la violencia desarrollan resilencia internamente y externamente. El artículo que a continuación se presenta, retoma de forma general los aspectos claves de la investigación finalizada sobre: comunicación, memoria y resiliencia, desarrollada por la Escuela de Comunicación social y periodismo de la Universidad Sergio Arboleda seccional Santa Marta.

Application of MODIS Products to Infer Possible Relationships Between Basin Land Cover and Coastal Waters Turbidity Using the Magdalena River, Colombia, as a Case Study

Science.gov (United States)

Madrinan, Max Jacobo Moreno; Cordova, Africa Flores; Olivares, Francisco Delgado; Irwin, Dan

2012-01-01

Basin development and consequent change in basin land cover have been often associated with an increased turbidity in coastal waters because of sediment yield and nutrients loading. The later leads to phytoplankton abundance further exacerbating water turbidity. This subsequently affects biological and physical processes in coastal estuaries by interfering with sun light penetration to coral reefs and sea grass, and even affecting public health. Therefore, consistent estimation of land cover changes and turbidity trend lines is crucial to design environmental and restoration management plans, to predict fate of possible pollutants, and to estimate sedimentary fluxes into the ocean. Ground solely methods to estimate land cover change would be unpractical and traditional methods of monitoring in situ water turbidity can be very expensive and time consuming. Accurate monitoring on the status and trends of basin land cover as well as the water quality of the receiving water bodies are required for analysis of relationships between the two variables. Use of remote sensing (RS) technology provides a great benefit for both fields of study, facilitating monitoring of changes in a timely and cost effective manner and covering wide areas with long term measurements. In this study, the Magdalena River basin and fixed geographical locations in the estuarine waters of its delta are used as a case to study the temporal trend lines of both, land cover change and the reflectance of the water turbidity using satellite technology. Land cover data from a combined product between sensors Terra and Aqua (MCD12Q1) from MODIS will be adapted to the conditions in the Magdalena basin to estimate changes in land cover since year 2000 to 2009. Surface reflectance data from a MODIS, Terra (MOD09GQ), band 1, will be used in lieu of in situ water turbidity for the time period between 2000 and present. Results will be compared with available existing data.

Geochemistry of Dissolved Trace Metals in the Waters of Bahia Magdalena, Baja California Sur, Pacific Coast, Mexico

Science.gov (United States)

Suresh Babu, S.

2016-12-01

Forty two samples were acquired from the surface and bottom water profiles along 5 transects spread over Bahia Magdalena lagoon, Baja California Sur to assess the behavior of trace metals in a high influenced upwelling region on the Pacific coast. To elaborate the fate of metals, also the physico-chemical parameters (pH, temperature, salinity, conductivity, dissolved oxygen). Determination of the concentrations of trace metals (Fe, Mn, Cr, Cu, Co, Pb, Ni, Zn, Cd As, Hg) were measured using Atomic absorption spectrometry. The results demonstrated high values of As, Ni and Co which is attributed to the local geology and phosphate deposits. Low values of Fe and Mn are attested to the oxic conditions of the lagoon which are responsible for the oxidation of Fe and Mn. The region witnesses raised temperatures (28.92ºC) and salinities of 35.2 PSU for its arid climatic conditions and high rates of evaporation. In general, the region presented minor quantities of dissolved trace metals due to dispersion and high intense interaction with the open sea. The results were also compared with other studies to understand the enrichment pattern in this side of the pacific coast which experiences various geothermal activities and upwelling phenomenon.

DNA gyrase with a single catalytic tyrosine can catalyze DNA supercoiling by a nicking-closing mechanism

Science.gov (United States)

Gubaev, Airat; Weidlich, Daniela; Klostermeier, Dagmar

2016-01-01

The topological state of DNA is important for replication, recombination and transcription, and is regulated in vivo by DNA topoisomerases. Gyrase introduces negative supercoils into DNA at the expense of ATP hydrolysis. It is the accepted view that gyrase achieves supercoiling by a strand passage mechanism, in which double-stranded DNA is cleaved, and a second double-stranded segment is passed through the gap, converting a positive DNA node into a negative node. We show here that gyrase with only one catalytic tyrosine that cleaves a single strand of its DNA substrate can catalyze DNA supercoiling without strand passage. We propose an alternative mechanism for DNA supercoiling via nicking and closing of DNA that involves trapping, segregation and relaxation of two positive supercoils. In contrast to DNA supercoiling, ATP-dependent relaxation and decatenation of DNA by gyrase lacking the C-terminal domains require both tyrosines and strand passage. Our results point towards mechanistic plasticity of gyrase and might pave the way for finding novel and specific mechanism-based gyrase inhibitors. PMID:27557712

PARÁMETROS GENÉTICOS PARA CARACTERÍSTICAS REPRODUCTIVAS EN UNA POBLACIÓN DE BÚFALOS (BUBALUS BUBALIS ARTIODACTYLA, BOVIDAE EN EL MAGDALENA MEDIO COLOMBIANO GENETIC PARAMETERS FOR REPRODUCTIVE TRAITS IN A POPULATION OF BUFFALOES (BUBALUS BUBALIS ARTIODACTYLA: BOVIDAE IN THE MIDDLE MAGDALENA REGION OF COLOMBIA

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Diana María Bolívar Vergara

2010-12-01

Full Text Available La investigación se hizo para estimar los componentes de (covarianza y parámetros genéticos para edad al primer parto (EPP e intervalo entre partos (IEP en una población bufalina en el Magdalena Medio Colombiano. Las características evaluadas fueron EPP, IEP entre primer y segundo parto (IEP1-2, IEP entre segundo y tercer parto (IEP2-3 , e IEP entre tercer y cuarto parto (IEP3-4 en un modelo animal multicaracterístico. El IEP con todos los partos (IEPtotal fue analizado en un modelo animal unicaracterístico. Las estimativas de los componentes de varianza fueron obtenidos por el método de máxima verosimilitud restringida libre de derivadas. Las heredabilidades encontradas fueron 0,42; 0,11; 0,07; 0,31 y 0,05±0,061 para EPP, IEP1-2 , IEP2-3 , IEP3-4 y IEPtotal, respectivamente. La heredabilidad encontrada para EPP, sugiere que la selección por esta característica es factible en esta población. La heredabilidad para IEPtotal indica que esta característica está muy influenciada por factores ambientales y genéticos no aditivos. Las correlaciones genéticas entre los diferentes IEP fueron altas y positivas. Las correlaciones genéticas entre EPP y los IEP variaron ampliamente.The research was carried out to estimate (covariance components and genetic parameters for age at first calving (AFC and calving interval (CI in a population of buffaloes located in Middle Magdalena region of Colombia. The traits evaluated were AFC, CI between first and second calving (CI1-2, CI between second and third calving (CI2-3 and CI between third and fourth calving(CI3-4, using animal model multitrait. A single-trait animal model was used for IEP considering all calving (CItotal. Estimative of (covariance components were obtained by the method of derivative free restricted maximum likelihood. Estimates of heritability were 0.42, 0.11, 0.07, 0.31, and 0.05 ± 0.061 for AFC, CI1-2, CI2-3, CI3-4, and CItotal, respectively. The heritability found for AFC

Avances en los objetivos de desarrollo del milenio en Santa Marta, Magdalena y Colombia (2005 – 2009

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Lidice Álvarez Miño

2013-10-01

Full Text Available ResumenCon el nuevo milenio los países miembros del sistema de Naciones Unidas decidieron establecer objetivos concretos, con metas específicas, para mejorar la vida de la población, principalmente de la más pobre. Colombia acogió dicha directriz y propuso metas nacionales a lograr en el 2015. El avance alcanzado en las metas está determinado por múltiples aspectos estructurales cuya expresión varía en cada ámbito territorial. Para identificar dichas diferencias, se realizó un estudio analítico-comparativo de los resultados obtenidos en Colombia, el Magdalena y Santa Marta para el periodo 2005 a 2009, con base en estadísticas del Instituto Nacional de Salud y del Departamento Administrativo Nacional Estadística. A partir del cálculo de tasas y razones se estableció que algunos logros registrados a nivel nacional, como la razón de mortalidad materna (pasando de 73X100.000nv en 2005 a 59X100.000nv en 2009 contrastan con dinámicas como la del Virus de Inmunodeficiencia Humana (para 2005 registró 3976 casos nuevos mientras para el 2009, 8094 casos nuevos. Además, se identificó la brecha existente entre el nivel nacional y el local. Se espera que estos resultados llamen la atención de los “decisores de políticas” para que, más allá del cumplimiento formal del objetivo, transformen elementos determinantes de la vida de la población implicada. (DUAZARY 2011, 17 - 23AbstractIn the new millennium, members of the United Nations system decided to set specific goals to improve life of the population, mainly the poorest people. Colombia welcomed this new guide and proposed own national targets to achieve in 2015. The progress made in each goal is determined by multiple structural features, whose expression varies by the territory (national, departmental and local. To identify these territorially variation, a comparative analytical study was performed using statistics from the National Institute of Health and National Bureau of

Estudio del valor indicador de las comunidades de algas bentónicas: una propuesta de evaluación y aplicación en el río Magdalena, Ciudad de México

OpenAIRE

Carmona Jiménez, Javier; Ramírez Rodríguez, Rocío; Bojorge-García, Miriam Guadalupe; González Hidalgo, Beatriz; Cantoral-Uriza, Enrique A.

2016-01-01

RESUMEN: La aplicación y desarrollo de las leyes ambientales que regulan el manejo de cuencas requiere la identificación de indicadores biológicos, los parámetros y las medidas para establecer su estado ecológico, para lo cual el presente estudio tiene como objetivo caracterizar el valor indicador ecológico de las algas bentónicas en una corriente urbana de la Ciudad de México. Se estudiaron seis sitios del río Magdalena en las estaciones más contrastantes del año: seca cálida, lluviosa y sec...

ISOLDE PH team, from left to right: Jennifer Weterings (user support), Susanne Kreim (research fellow), Marek Pfützner (scientific associate), Maria Garcia Borge (team leader), Elisa Rapisarda (research fellow) , Magdalena Kowalska (physics coordinator), Jan Kurcewicz (applied fellow), Monika Stachura (applied fellow). Not in the photo: Kara Lynch (PhD student).

CERN Multimedia

Visual Media Office

2013-01-01

ISOLDE PH team, from left to right: Jennifer Weterings (user support), Susanne Kreim (research fellow), Marek Pfützner (scientific associate), Maria Garcia Borge (team leader), Elisa Rapisarda (research fellow) , Magdalena Kowalska (physics coordinator), Jan Kurcewicz (applied fellow), Monika Stachura (applied fellow). Not in the photo: Kara Lynch (PhD student).

Relationship between petrographic pore types and core measurements in sandstones of the Monserrate Formation, upper Magdalena Valley, Colombia

International Nuclear Information System (INIS)

Ehrlich, R; Cobaleda, G; Barclay, Ferm

1997-01-01

Patterns of porosity in sandstones of the Monserrate Formation (Upper Magdalena Valley) exposed in polished blocks have been digitally recorded using an image processor coupled to a scanning electron microscope operated in back scatter electron mode. Additionally, porosity, permeability and response to mercury injection-capillary pressure tests were measured on some of the imaged samples. Porosity pattern were evaluated via an erosion/dilation-differencing image-processing algorithm and then classified by the self-training classifier, SAWVEC. Changes in the resulting pore type proportions were strongly associated with changes in the mercury porosimetry curves. From the image processing data, five pore types, sufficient to include all of the variability in size and shape of the patterns of porosity, were identified. Variations in the number of pares of each type per unit cross sectional area were related to variations in permeability. The resultant relationships with mercury porosimetry demonstrated that pares of the same type tends to form microcircuits characterized by a limited throat size range. Permeability modeling showed that intergranular Pare Types 2 and 4 (secondary porosity resulting from carbonate dissolution) are responsible for permeability in the 0,01 - 0,1 0 Darcy range. Type 5 pares (large molds) slightly contribute to permeability, except in coarse grained rocks where they are efficiently connected by micro fractures

Manejo de la primera alimentación del bocachico ( Prochilodus magdalenae.

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Víctor Atencio-García

2003-06-01

Full Text Available En el Centro de Investigación Piscícola de la Universidad de Córdoba se realizaron dos experimentos; en elprimero, el objetivo fue evaluar el efecto de diferentes presas en el manejo de la primera alimentación delbocachico Prochilodus magdalenae y en el segundo, con la mejor presa del ensayo anterior, determinar eltiempo mínimo de manejo de la primera alimentación. En ambos casos las post-larvas fueron sembradas a 50/L en acuario de 5 L. En el primer experimento se evaluaron las siguientes presas: nauplios de Artemia (NA,cistos descapsulados de Artemia (CA, zooplancton silvestre tamizado entre 125-160 µm (Z125-160 y zooplanctonsilvestre tamizado entre 250-400 µm (Z250-400, ofrecidas durante cinco días a razón de 5 Presa/mL. Además,un grupo de post-larvas fue sometida a ayuno. Las mayores ganancias de peso y longitud total la registraron laspost-larvas alimentadas con NA (2.0±1.8 mg, 1.8±0.9 mm y Z250-400 (2.0±1.1 mg, 1.6±0.5 mm. Lasobrevivencia de las post-larvas alimentadas con NA (73.2±10.7% fue mayor a las registradas en los demástratamientos (P0.05. Mientras que latasa de crecimiento específico (G osciló entre 17.1 (5 días y 22.3 %/día (3 días, sin observarse diferenciasestadísticas significativas (P>0.05. Las post-larvas alimentadas durante 3, 5 y 7 días presentaron las mejoressobrevivencias (92.2±6.2, 93.3±4.3, 95.6±3.9% respectivamente cuando fueron sometidas a la prueba deresistencia al estrés. Las post-larvas alimentadas por un solo día mostraron la menor sobrevivencia al sersometidas a esta prueba (87±1%. Los resultados del estudio sugieren que nauplios de Artemia o zooplanctonsilvestre en le rango de 250-400 mm, libre de predadores, son presas adecuada para el manejo de la primeraalimentación al menos durante 3 días antes de ser sembradas en estanques en tierra.

Konstrukce vřeten vícevřetenového soustružnického automatu

OpenAIRE

Kráčmar, Tomáš

2016-01-01

Obsahem diplomové práce je konstrukce vřeten vícevřetenového soustružnického automatu pro práci z tyče maximálního průměru 7 mm. Práce se zabývá novou koncepcí pohonu vřeten, kde jsou vřetena poháněna externími asynchronními motory přes ozubená kola s vnitřním ozubením uložená vně vřetenového bubnu namísto současného způsobu pohonu centrálními koaxiálními hřídeli. Součástí práce je rešerše vícevřetenových automatů včetně popisu hlavních uzlů, konstrukční návrh, pevnostní výpočty, výpočty trva...

ANÁLISIS HIDROLÓGICO E HIDRÁULICO DE LA CUENCA DEL RÍO FRÍO, MUNICIPIOS DE CIÉNAGA Y ZONA BANANERA, DEPARTAMENTO DEL MAGDALENA

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Alfredo Ramos Moreno

2017-07-01

Full Text Available Los modelos hidrológicos e hidráulicos han permitido grandes avances en los estudios de las cuencas hidrográficas, la utilización de modelos como HEC RAS y HEC HMS los cuales han recibido una gran acogida por la comunidad mundial de ingenieros que se desempeñan en el área de la meteorología, hidrología, hidráulica y otras áreas relacionadas con el manejo del recurso hídrico. Gracias a sus grandes aproximaciones los modelos de HEC RAS y HEC HMS fueron seleccionados para realizar las modelaciones para determinar el comportamiento hidrológico e hidráulico de la cuenca del río Frío ubicada en el municipio de Zona Bananera, departamento del Magdalena como producto de las inundaciones que se han presentado en los últimos años. En este artículo se expone una cantidad de análisis cuantitativos y cualitativos que posibilitan la obtención de la información de entrada de los modelos de simulación, posteriormente, dicha información fue introducida a los modelos para lograr determinar el comportamiento bajo escenarios extremos a los que la cuenca del río Frío puede ser sometida. De acuerdo con los resultados obtenidos las áreas donde se presentan los principales inconvenientes de carácter hidráulico e hidrológico, se deben principalmente a la configuración geométrica de la batimetría del río y al desarrollo de actividades agrícolas y ganaderas que han generado cambios en el uso del suelo, modificando los números de curva y aumentando la escorrentía hacia los afluentes y finalmente hacia el río principal. Frente a esta situación se relacionan una serie de recomendaciones que buscan corregir el régimen hidrológico y comportamiento hidrodinámico para evitar nuevas inundaciones que pongan en riesgo a la población que se encuentra en las inmediaciones del río Frío en el municipio de la Zona Bananera Departamento del Magdalena.

An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplification.

Science.gov (United States)

Xu, Jingguo; Guo, Jia; Maina, Sarah Wanjiku; Yang, Yumeng; Hu, Yimin; Li, Xuanxuan; Qiu, Jiarong; Xin, Zhihong

2018-05-15

An ultra-sensitive aptamer-based biosensor for the detection of staphylococcus aureus was established by adopting the nicking enzyme amplification reaction (NEAR) and the rolling circle amplification (RCA) technologies. Aptamer-probe (AP), containing an aptamer and a probe sequence, was developed to act as the recognition unit of the biosensor, which was specifically bound to S. aureus. The probe was released from AP and initiated into the subsequent DNA amplification reactions where S. aureus was present, converting the detection of S. aureus to the investigation of probe oligonucleotide. The RCA amplification products contained a G-quadruplex motif and formed a three dimensional structure in presence of hemin. The G4/hemin complex showed horseradish peroxidase (HRP)-mimic activity and catalyzed the chemiluminescence reaction of luminol mediated by H 2 O 2 . The results showed that the established biosensor could detect S. aureus specifically with a good linear correlation at 5-10 4  CFU/mL. The signal values based on NEAR-RCA two-step cycle were boosted acutely, much higher than that relied on one-cycle magnification. The limit of detection (LoD) was determined to be as low as 5 CFU/mL. The established aptasensor exhibited a good discrimination of living against dead S. aureus, and can be applied to detect S. aureus in the food industry. Copyright © 2018 Elsevier Inc. All rights reserved.

Assessing bovine babesiosis in Rhipicephalus (Boophilus microplus ticks and 3 to 9-month-old cattle in the middle Magdalena region, Colombia

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Sandra Ríos-Tobón

Full Text Available Babesia sp. is a protozoan hemoparasite that affects livestock worldwide. The Colombian Middle Magdalena is an enzootic region for babesiosis, but there is no previous research providing detail on its transmission cycle. This study aims to assess some Babesia sp. infection indicators in cattle and ticks from the area, by using direct microscopic and molecular techniques to detect the infection. In the cattle, 59.9% and 3.4 % positivity values for B. bigemina and mixed infection (B. bovis + B. bigemina were found respectively. In ticks, the positivity of B. bigemina reached 79.2% and 9.4% for the mixed infection. The degree of infestation in the region was 3.2 ticks per bovine. There was positive correlation between tick control acaricide frequencies and infestation in bovines. This leads us to infer that control periodicity greater than 90 days, in stable zones, is an abiotic factor that benefits the acquisition of protective immunity in calves, the natural control of the infection and eventual disease absence. It is necessary to monitor the disease by applying new entomological and parasitological indicators showing the complexity of this phenomenon.

Percepciones estudiantiles sobre el proceso de acreditación por alta calidad del programa de administración de empresas de la universidad del magdalena

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Javier Viloria-Escobar

2015-08-01

Full Text Available This article aimed to explore the perceptions of students of Business Administration Program of the University of Magdalena on the accreditation process for high quality that is submitted to the Ministry of Education. For which a random sample of 100 students was taken, who are part of various academic semesters, answered a questionnaire that asked for their views on the process. The main results support the view that students have a huge sense of belonging to the program and believe that it deserves accreditation by the Ministry and, further, this certificate becomes something positive for their careers. We conclude that, despite the apparent acceptance of the conditions of program quality, it is necessary that efforts be brought forward to create a culture of quality throughout the student population and a and a nationwide study is proposed to investigate these perceptions in various universities in Colombia, having as reference the implications of the context to which they belong.

Optimization of a DNA Nicking Assay to Evaluate Oenocarpus bataua and Camellia sinensis Antioxidant Capacity

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Louis-Jérôme Leba

2014-10-01

Full Text Available This study was aimed at assessing the DNA damage protective activity of different types of extracts (aqueous, methanolic and acetonic using an in vitro DNA nicking assay. Several parameters were optimized using the pUC18 plasmid, especially FeSO4, EDTA, solvent concentrations and incubation time. Special attention has been paid to removing the protective and damaging effect of the solvent and FeSO4 respectively, as well as to identifying the relevant positive and negative controls. For each solvent, the optimal conditions were determined: (i for aqueous extracts, 0.33 mM of FeSO4 and 0.62 mM of EDTA were incubated for 20 min at 37 °C; (ii for acetone extracts, 1.16% solvent were incubated for 15 min at 37 °C with 1.3 mM of FeSO4 and 2.5 mM of EDTA and (iii for methanol extracts, 0.16% solvent, were incubated for 1.5 h at 37 °C with 0.33 mM of FeSO4 and 0.62 mM of EDTA. Using the optimized conditions, the DNA damage protective activity of aqueous, methanolic and acetonic extracts of an Amazonian palm berry (Oenocarpus bataua and green tea (Camellia sinensis was assessed. Aqueous and acetonic Oenocarpus bataua extracts were protective against DNA damage, whereas aqueous, methanolic and acetonic extracts of Camellia sinensis extracts induced DNA damage.

DESIGN AND TEST OF AN INSTRUMENT FOR MEASURING MICROTHERMAL SEEING ON THE MAGDALENA RIDGE

International Nuclear Information System (INIS)

Jorgensen, A. M.; Klinglesmith, D. A.; Speights, J.; Clements, A.; Patel, J.

2009-01-01

We have constructed and operated an automated instrument for measuring ground-level microthermal seeing at the Magdalena Ridge Observatory (MRO). The MRO is located at an altitude of 10500' in the Cibola National Forest in New Mexico, USA. It is the planned site for the MRO Optical Interferometer (MROI) planned for up to 10 collecting elements, each with a diameter of 1.4 m, and baselines eventually up to approximately 400 m. As part of the preparation for construction we deployed a system to characterize the ground-level seeing across the observatory site. The instrument is built largely of off-the-shelf components, with only the sensor head and power supply requiring electronic board assembly. Even in those cases the board architecture is very simple. The first proof-of-concept system was deployed for several weeks in the autumn of 2004, and has since undergone several iterations. The latest configuration operates entirely off batteries, incorporates wireless data acquisition, and is thus able to operate in an area with no shelter, power, or communications. In this paper we present the design of the instrument, and show initial data. The microthermal tower has four sensor pairs at heights from 0.8 to 4.41 m, significantly lower than other microthermal experiments, because of the need to characterize the seeing near the ground. We find significant variation in the contribution of this range of heights to the seeing, contributing up to 0.''3 of the seeing at some times and only 0.''02 at other times. The individual sensor power spectra have a slope in the range of 1.4--1.5, which is lower than the 1.67 slope predicted by Kolmogorov turbulence theory. We measure the well known effect of improved seeing immediately around sunset. While we find significant variation in the microthermal seeing, we did not find a pattern of corresponding variations in weather conditions, suggesting that a complicated set of factors control microthermal turbulence.

Design and Test of an Instrument for Measuring Microthermal Seeing on the Magdalena Ridge

Science.gov (United States)

Jorgensen, A. M.; Klinglesmith, D. A., III; Speights, J.; Clements, A.; Patel, J.

2009-05-01

We have constructed and operated an automated instrument for measuring ground-level microthermal seeing at the Magdalena Ridge Observatory (MRO). The MRO is located at an altitude of 10500' in the Cibola National Forest in New Mexico, USA. It is the planned site for the MRO Optical Interferometer (MROI) planned for up to 10 collecting elements, each with a diameter of 1.4 m, and baselines eventually up to approximately 400 m. As part of the preparation for construction we deployed a system to characterize the ground-level seeing across the observatory site. The instrument is built largely of off-the-shelf components, with only the sensor head and power supply requiring electronic board assembly. Even in those cases the board architecture is very simple. The first proof-of-concept system was deployed for several weeks in the autumn of 2004, and has since undergone several iterations. The latest configuration operates entirely off batteries, incorporates wireless data acquisition, and is thus able to operate in an area with no shelter, power, or communications. In this paper we present the design of the instrument, and show initial data. The microthermal tower has four sensor pairs at heights from 0.8 to 4.41 m, significantly lower than other microthermal experiments, because of the need to characterize the seeing near the ground. We find significant variation in the contribution of this range of heights to the seeing, contributing up to 0farcs3 of the seeing at some times and only 0farcs02 at other times. The individual sensor power spectra have a slope in the range of 1.4--1.5, which is lower than the 1.67 slope predicted by Kolmogorov turbulence theory. We measure the well known effect of improved seeing immediately around sunset. While we find significant variation in the microthermal seeing, we did not find a pattern of corresponding variations in weather conditions, suggesting that a complicated set of factors control microthermal turbulence.

Ultrasensitive electrochemical biosensor for detection of DNA from Bacillus subtilis by coupling target-induced strand displacement and nicking endonuclease signal amplification.

Science.gov (United States)

Hu, Yuhua; Xu, Xueqin; Liu, Qionghua; Wang, Ling; Lin, Zhenyu; Chen, Guonan

2014-09-02

A simple, ultrasensitive, and specific electrochemical biosensor was designed to determine the given DNA sequence of Bacillus subtilis by coupling target-induced strand displacement and nicking endonuclease signal amplification. The target DNA (TD, the DNA sequence from the hypervarient region of 16S rDNA of Bacillus subtilis) could be detected by the differential pulse voltammetry (DPV) in a range from 0.1 fM to 20 fM with the detection limit down to 0.08 fM at the 3s(blank) level. This electrochemical biosensor exhibits high distinction ability to single-base mismatch, double-bases mismatch, and noncomplementary DNA sequence, which may be expected to detect single-base mismatch and single nucleotide polymorphisms (SNPs). Moreover, the applicability of the designed biosensor for detecting the given DNA sequence from Bacillus subtilis was investigated. The result obtained by electrochemical method is approximately consistent with that by a real-time quantitative polymerase chain reaction detecting system (QPCR) with SYBR Green.

TRANSFORMACIONES ALREDEDOR DEL DISCURSO, PRÁCTICAS EDUCATIVAS, ACTITUDES Y RELACIONES SOCIALES DE LAS MADRES COMUNITARIAS DEL ICBF. "Pueblo Viejo" (Magdalena y el programa "Nichos Pedagógicos"

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Mónica Borjas

2009-01-01

Full Text Available Este artículo presenta los resultados de un proceso de investigación que da cuenta de las transformaciones que las madres comunitarias tuvieron en torno a su discurso, práctica educativa y relaciones sociales, en el seno del programa de capacitación "Nichos Pedagógicos", orientado por el Instituto de Estudios en Educación iese, de la Universidad del Norte, bajo el patrocinio de la fundación promigas. Inicialmente, se exponen los referentes contextuales relacionados con los aspectos misionales del Instituto Colombiano de Bienestar Familiar -icbf-y con el municipio de Pueblo Viejo, Magdalena (Colombia, lugar de origen de las madres comunitarias participantes. En segunda instancia, se presentan los referentes de teóricos de las categorías de análisis: Discurso, prácticas e interacciones y, finalmente, se expone una conceptualización de "Nicho Pedagógico", denominación del Programa de Capacitación ofrecido a las madres comunitarias.

Fauna silvestre asociada a ganado vacuno doble propósito en sistema de silvopastoreo, Pinto, Magdalena, Colombia

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Jaime De La Ossa V.

2011-12-01

Full Text Available Objetivo. Evaluar la biodiversidad faunística asociada a ganadería vacuna de doble propósito en dos potreros estructurados con Botriochloa pertusa (colosoana como herbácea dominante, uno de ellos plantado con Crescentia cujete (Bignoniaceae como fuente de alimento adicional y otro con escaso y disperso arbolado. Materiales y métodos. El área de estudio está ubicada en el municipio de Pinto, Magdalena. Cada potrero evaluado fue de 10 ha, se mantuvo en cada uno un lote de quince vacas (½ Cebú, ¼ Pardo y ¼ Costeño con Cuernos, la separación entre las áreas muestreadas fue de 500m. Los registros de fauna se condujeron mediante punto fijo de conteo, en diez sitios de avistamiento, durante 20 días continuos, con cubrimiento visual amplio. Al ganado vacuno adulto presente en las dos áreas de trabajo se le realizó un pesaje individual al inicio del trabajo y al final del mismo, con el fin de determinar posibles diferencias entre ellos. Resultados. Estadísticamente el número de individuos y el número de especies presentaron diferencias significativas, siendo mayor la diversidad biológica para el potrero que poseía la plantación de C. cujete, mientras que el peso corporal de las vacas sometidas a pastoreo en las dos áreas no presentó diferencias significativas. Conclusiones. Se demuestra la importancia de este modelo pecuario como promotor de la conservación faunística local al convertirse enrefugio de variadas especies silvestres, al tiempo que mantiene la productividad existente sin alterar el ambiente sustancialmente.

Secado de trozos de yuca por convección natural en los valles de los ríos Cauca y Magdalena

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Alfonso Parra Coronado

1992-09-01

Full Text Available En este artículo se presentan los resultados obtenidos en la evaluación del potencial de secado de yuca en trozos por convección natural en los Valles de los ríos Cauca y Magdalena, utilizando la metodología desarrollada en el Departamento de Ingeniería Agrícola de la Universidad Nacional de Colombia sede Santafé de Bogotá, D.C., por Domínguez J. y Parra A. (1982, dentro de la línea de investigación "Secado y almacenamiento de Productos Agrícolas". La metodología se basa en la técnica de simulación matemática, mediante la cual es posible predecir simultáneamente, la variación meteorológica, el proceso de secado y el proceso de deterioración del producto, con el fin de optimizar el manejo y funcionamiento de sistemas de secado solar y natural. El modelo de simulación se compone de: el modelo de secado natural desarrollado por Roa (1974, el modelo de deterioro desarrollado por Ospina (1979 y de una rutina de interpolación polinomial que permite obtener la carga máxima de yuca (Kg/m2 que se puede secar sin que se deteriore más del 15%.

DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae sperm following cryopreservation with dimethylsulfoxide and glucose

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José Gregorio Martínez

Full Text Available The endangered bocachico Prochilodus magdalenae is a native freshwater fish of Colombia, the most captured species locally and one of the most important species for ex-situ conservation (germplasm banks. The aim of this study was to examine the effect of three concentrations of Dimethylsulfoxide (DMSO (5%, 10%, 15% and three of glucose (305, 333, 361 mM in the extender on spermatic DNA fragmentation (F-DNA (by Halomax®, Chromatin dispersion and membrane damage (D-Me (by eosin-nigrosin staining. After assessment of sperm quality by computer analysis of motility, one part of semen from males was diluted separately with three parts of extender and filled into 0.5 ml straws. Freezing was carried out in liquid nitrogen vapor dry shipper for 30 minutes and thawed at 60ºC for 8 seconds in a water bath and evaluated for the percentage of cells found with F-DNA and D-Me. The results demonstrated that cryopreservation causes greater F-DNA (13.62 ± 1.6% to 28.91 ± 3.25 and D-Me (24.27 ± 1.1% to 58.33 ± 2.81% when compared with pre-freezing semen (PFS (6.71 ± 1.54% and 2.34 ± 0.5%, respectively for F-DNA and D-Me. A significant interaction was found between DMSO and glucose concentration in this experiment. Use of extender: 10% DMSO + 305 mM glucose + 12% chicken egg yolk and, 10% DMSO + 333 mM glucose + 12% chicken egg yolk, allow for lower F-DNA and D-Me during cryopreservation of bocachico semen. A high correlation between F-DNA and D-Me was found (r = 0.771.

DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae sperm following cryopreservation with dimethylsulfoxide and glucose

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José Gregorio Martínez

2012-09-01

Full Text Available The endangered bocachico Prochilodus magdalenae is a native freshwater fish of Colombia, the most captured species locally and one of the most important species for ex-situ conservation (germplasm banks. The aim of this study was to examine the effect of three concentrations of Dimethylsulfoxide (DMSO (5%, 10%, 15% and three of glucose (305, 333, 361 mM in the extender on spermatic DNA fragmentation (F-DNA (by Halomax®, Chromatin dispersion and membrane damage (D-Me (by eosin-nigrosin staining. After assessment of sperm quality by computer analysis of motility, one part of semen from males was diluted separately with three parts of extender and filled into 0.5 ml straws. Freezing was carried out in liquid nitrogen vapor dry shipper for 30 minutes and thawed at 60ºC for 8 seconds in a water bath and evaluated for the percentage of cells found with F-DNA and D-Me. The results demonstrated that cryopreservation causes greater F-DNA (13.62 ± 1.6% to 28.91 ± 3.25 and D-Me (24.27 ± 1.1% to 58.33 ± 2.81% when compared with pre-freezing semen (PFS (6.71 ± 1.54% and 2.34 ± 0.5%, respectively for F-DNA and D-Me. A significant interaction was found between DMSO and glucose concentration in this experiment. Use of extender: 10% DMSO + 305 mM glucose + 12% chicken egg yolk and, 10% DMSO + 333 mM glucose + 12% chicken egg yolk, allow for lower F-DNA and D-Me during cryopreservation of bocachico semen. A high correlation between F-DNA and D-Me was found (r = 0.771.O curimba Prochilodus magdalenae, é uma espécie nativa de água doce da Colômbia ameaçada de extinção, sendo a mais capturada localmente e uma das mais importantes para a conservação ex-situ (bancos de germoplasma. O objetivo deste estudo foi avaliar o efeito de três concentrações de dimetilsulfóxido (DMSO (5%, 10%, 15% e três de glicose (305, 333, 361 mM no diluente sobre a fragmentação do ADN espermático (F-DNA (através de Halomax®, dispersão da cromatina e danos em

Formas de carbono orgánico en suelos con diferentes usos en el departamento del Magdalena (Colombia

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Menjivar Flores Juan Carlos

2011-12-01

Full Text Available Las fracciones de materia orgánica del suelo (MOS lábiles y humificadas pueden ser afectadas por las prácticas de uso y manejo; sin embargo el impacto de estos cambios no se ha evaluado en suelos y ambientes tropicales. El presente estudio tuvo como objetivo evaluar los contenidos y algunas formas de carbono orgánico del suelo (COS en cinco zonas de clima cálido tropical (0 - 1110 m.s.n.m. del departamento del Magdalena (Colombia y el efecto que sobre ellas han tenido las prácticas asociadas a suelos cultivados con café (Coffea arabica, banano (Musa sp., palma africana (Elaeis guineensis y sábila (Aloe vera, comparados con suelos de bosques naturales. No se encontraron diferencias (P < 0.05 tanto entre zonas como entre usos del suelo, se presentaron valores medios a bajos de MOS en las zonas de estudio y contenidos de carbono total (Ct mayores en suelos de bosques que en suelos cultivados, así los suelos de bosques presentan una acumulación media de Ct de 42.4 mg/ha a 20 cm, frente a 33.8 mg/ha en los suelos cultivados, esto equivale a una pérdida media de Ct del 23% por efecto del manejo de los cultivos. En relación con el carbono extraíble con pirofosfato sódico altamente relacionado con las fracciones humificadas de la MOS (Cp se observaron valores muy bajos en los suelos cultivados y casi nulos en suelos de bosques; sin embargo estos últimos presentan mayor contenido de formas de carbono no-oxidables o estables (Cnox determinado por diferencia entre Ct - carbono oxidable (Cox. En el suelo cultivado con banano, el Ct corresponde en su totalidad a formas de Cox; mientras que en el suelo cultivado con palma africana las formas estables Cnox representaron 83% del carbono total.

Formas de carbono orgánico en suelos con diferentes usos en el departamento del Magdalena (Colombia

Directory of Open Access Journals (Sweden)

José Rafael Vásquez-Polo

2011-12-01

Full Text Available Las fracciones de materia orgánica del suelo (MOS lábiles y humificadas pueden ser afectadas por las prácticas de uso y manejo; sin embargo el impacto de estos cambios no se ha evaluado en suelos y ambientes tropicales. El presente estudio tuvo como objetivo evaluar los contenidos y algunas formas de carbono orgánico del suelo (COS en cinco zonas de clima cálido tropical (0 - 1110 m.s.n.m. del departamento del Magdalena (Colombia y el efecto que sobre ellas han tenido las prácticas asociadas a suelos cultivados con café (Coffea arabica, banano (Musa sp., palma africana (Elaeis guineensis y sábila (Aloe vera, comparados con suelos de bosques naturales. No se encontraron diferencias (P < 0.05 tanto entre zonas como entre usos del suelo, se presentaron valores medios a bajos de MOS en las zonas de estudio y contenidos de carbono total (Ct mayores en suelos de bosques que en suelos cultivados, así los suelos de bosques presentan una acumulación media de Ct de 42.4 mg/ha a 20 cm, frente a 33.8 mg/ha en los suelos cultivados, esto equivale a una pérdida media de Ct del 23% por efecto del manejo de los cultivos. En relación con el carbono extraíble con pirofosfato sódico altamente relacionado con las fracciones humificadas de la MOS (Cp se observaron valores muy bajos en los suelos cultivados y casi nulos en suelos de bosques; sin embargo estos últimos presentan mayor contenido de formas de carbono no-oxidables o estables (Cnox determinado por diferencia entre Ct - carbono oxidable (Cox. En el suelo cultivado con banano, el Ct corresponde en su totalidad a formas de Cox; mientras que en el suelo cultivado con palma africana las formas estables Cnox representaron 83% del carbono total.

Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform.

Science.gov (United States)

Li, Xiang; Ding, Xuelian; Fan, Jing

2015-12-07

Sensitive and selective detection of ultralow concentrations of specific biomolecules is important in early clinical diagnoses and biomedical applications. Many types of aptasensors have been developed for the detection of various biomolecules, but usually suffer from false positive signals and high background signals. In this work, we have developed an amplified fluorescence aptasensor platform for ultrasensitive biomolecule detection based on enzyme-assisted target-recycling signal amplification and graphene oxide. By using a split molecular aptamer beacon and a nicking enzyme, the typical problem of false positive signals can be effectively resolved. Only in the presence of a target biomolecule, the sensor system is able to generate a positive signal, which significantly improves the selectivity of the aptasensor. Moreover, using graphene oxide as a super-quencher can effectively reduce the high background signal of a sensing platform. We select vascular endothelial growth factor (VEGF) and adenosine triphosphate (ATP) as model analytes in the current proof-of-concept experiments. It is shown that under optimized conditions, our strategy exhibits high sensitivity and selectivity for the quantification of VEGF and ATP with a low detection limit (1 pM and 4 nM, respectively). In addition, this biosensor has been successfully utilized in the analysis of real biological samples.

Estudio para la medición de seguridad alimentaria y nutricional en el Magdalena medio colombiano. Caso Cesar.

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Sara Del Castillo M.

2012-03-01

Full Text Available Antecedentes. El presente artículo presenta los resultados del Estudio para la Medición de la Seguridad Alimentaria y Nutricional  (SAN en el Magdalena Medio Colombiano. Caso César, realizado por Obusinga y el OBSAN, financiado por OBUSINGA y por la UGI de la Facultad de Medicina de la Universidad Nacional de Colombia. Adicionalmente, forma parte de las acciones del Proyecto de Construcción Participativa de una Propuesta de Desarrollo Territorial con Perspectiva Alimentaria en 12 Municipios del Departamento del Cesar. Objetivo. Determinar el nivel de inseguridad alimentaria y nutricional de la población de los 12  municipios. Material y métodos.  El estudio se basó en las referencias establecidas por el Instituto de Nutrición de Centroamérica y Panamá, la metodología utilizada corresponde a la diseñada por la Universidad Nacional de Colombia para los estudios de Canasta de Alimentos desde el 2003, adaptada para este estudio por la Corporación  Obusinga. Resultados. Los principales resultados de la investigación permitieron reconocer algunas características sociodemográficas de la población objeto, identificar la Canasta Alimentaria Básica de los municipios seleccionados, estableciendo la Canasta Usual de Alimentos, canasta alimentaria recomendada, y la línea de indigencia y pobreza de los  hogares. Conclusión. La importancia de dichos resultados dejan en evidencia los altos niveles de inseguridad alimentaria de la población de los municipios del sur del departamento del César trabajados, los cuales se ven reflejados en los niveles de adecuación de calorías y nutrientes, el alto porcentaje de hogares bajo las líneas de pobreza e indigencia, constituyéndose en una herramienta básica para orientar la formulación o ajuste de los planes locales de SAN en la Región.

DNA scanning mechanism of T4 endonuclease V. Effect of NaCl concentration on processive nicking activity

International Nuclear Information System (INIS)

Gruskin, E.A.; Lloyd, R.S.

1986-01-01

T4 endonuclease V is a pyrimidine dimer-specific endonuclease which generates incisions in DNA at the sites of pyrimidine dimers by a processive reaction mechanism. A model is presented in which the degree of processivity is directly related to the efficacy of the one-dimensional diffusion of endonuclease V on DNA by which the enzyme locates pyrimidine dimers. The modulation of the processive nicking activity of T4 endonuclease V on superhelical covalently closed circular DNA (form I) which contains pyrimidine dimers has been investigated as a function of the ionic strength of the reaction. Agarose gel electrophoresis was used to separate the three topological forms of the DNA which were generated in time course reactions of endonuclease V with dimer-containing form I DNA in the absence of NaCl, and in 25, 50, and 100 mM NaCl. The degree of processivity was evaluated in terms of the mass fraction of form III (linear) DNA which was produced as a function of the fraction of form I DNA remaining. Processivity is maximal in the absence of NaCl and decreases as the NaCl concentration is increased. At 100 mM NaCl, processivity is abolished and endonuclease V generates incisions in DNA at the site of dimers by a distributive reaction mechanism. The change from the distributive to a processive reaction mechanism occurs at NaCl concentrations slightly below 50 mM. The high degree of processivity which is observed in the absence of NaCl is reversible to the distributive mechanism, as demonstrated by experiments in which the NaCl concentration was increased during the time course reaction. In addition, unirradiated DNA inhibited the incision of irradiated DNA only at NaCl concentrations at which processivity was observed

La literatura en la frontera lingüística : el caso de la escritora italo-suiza Fleur Jaeggy

OpenAIRE

Soria Millán, María del Pilar

2012-01-01

"El verdadero milagro proustiano no es que una magdalena mojada en té tenga el mismo gusto que otra magdalena mojada en té y despierte el recuerdo; es, más bien, que esa segunda magdalena resucite con ella un cuarto, una casa, una ciudad entera, y que ese lugar antiguo pueda, por espacio de un segundo, conmover la solidez del lugar actual, forzar sus puertas y hacer vacilar sus muebles." [1] Genette aporta con estas palabras la idea principal de lo que en esta tesis se pretende analizar, ...

Molecular systematics of the freshwater stingrays (myliobatiformes: potamotrygonidae) of the Amazon, Orinoco, Magdalena, Esequibo, Caribbean, and Maracaibo basins (Colombia - Venezuela): evidence from three mitochondrial genes.

Science.gov (United States)

Garcia, David Alejandro; Lasso, Carlos Andres; Morales, Monica; Caballero, Susana Josefina

2016-11-01

Lack of adequate information about the taxonomic and evolutionary relationships, ecology, biology, and distribution of several species belonging to the family Potamotrygonidae makes these species vulnerable to anthropic activities, including commercial overexploitation for the ornamental fish market. The aim of this study was to investigate the systematic relationships among genera and species belonging to this family by analyses of three mitochondrial gene regions. Samples were collected from the main river basins in Colombia and Venezuela for four genera and seven species of the family, as well as for what appear to be unidentified species. Three mitochondrial molecular markers COI, Cytb, and ATP6 were amplified and sequenced. Maximum likelihood and Bayesian inference analysis were performed to obtain topologies for each marker and for a concatenated dataset including the three genes. Small dataset may compromise some methods estimations of sequence divergence in the ATP6 marker. Monophyly of the four genera in Potamotrygonidae was confirmed and phylogenetic relationships among members of the Potamotrygon genus were not clearly resolved. However, results obtained with the molecular marker Cytb appear to offer a good starting point to differentiate among genera and species as a tool that could be used for barcoding. The application of this gene as a barcode could be applied for management and regulation of extraction practices for these genera. Sequencing complete mitochondrial genomes would be the next step for testing evolutionary hypothesis among these genera. Population structure analyses should be undertaken for Paratrygon, Potamotrygon magdalenae and motoro.

Analysis of Apoptosis in Ultraviolet-Induced Sea Cucumber (Stichopus japonicus) Melting Using Terminal Deoxynucleotidyl-Transferase-Mediated dUTP Nick End-Labeling Assay and Cleaved Caspase-3 Immunohistochemistry.

Science.gov (United States)

Yang, Jing-Feng; Gao, Rong-Chun; Wu, Hai-Tao; Li, Peng-Fei; Hu, Xian-Shu; Zhou, Da-Yong; Zhu, Bei-Wei; Su, Yi-Cheng

2015-11-04

The sea cucumber body wall melting phenomenon occurs under certain circumstances, and the mechanism of this phenomenon remains unclear. This study investigated the apoptosis in the ultraviolet (UV)-induced sea cucumber melting phenomenon. Fresh sea cucumbers (Stichopus japonicus) were exposed to UV radiation for half an hour at an intensity of 0.056 mW/cm(2) and then held at room temperature for melting development. The samples were histologically processed into formalin-fixed paraffin-embedded tissues. The apoptosis of samples was analyzed with the terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling (TUNEL) assay and cleaved caspase-3 immunohistochemistry. The emergence of TUNEL-positive cells speeds up between 0.5 and 2 h after UV irradiation. Cleaved caspase-3 positive cells were obviously detected in sample tissues immediately after the UV irradiation. These results demonstrated that sea cucumber melting induced by UV irradiation was triggered by the activation of caspase-3 followed by DNA fragmentation in sea cucumber tissue, which was attributed to apoptosis but was not a consequence of autolysis activity.

Combination of RNAseq and SNP nanofluidic array reveals the center of genetic diversity of cacao pathogen Moniliophthora roreri in the upper Magdalena Valley of Colombia and its clonality

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Shahin S Ali

2015-08-01

Full Text Available Moniliophthora roreri is the fungal pathogen that causes frosty pod rot (FPR disease of Theobroma cacao L., the source of chocolate. FPR occurs in most of the cacao producing countries in the Western Hemisphere, causing yield losses up to 80%. Genetic diversity within the FPR pathogen population may allow the population to adapt to changing environmental conditions and adapt to enhanced resistance in the host plant. The present study developed SNP markers from RNASeq results for 13 M. roreri isolates and validated the markers for their ability to reveal genetic diversity in an international M. roreri collection. The SNP resources reported herein represent the first study of RNASeq-derived SNP validation in M. roreri and demonstrates the utility of RNASeq as an approach for de novo SNP identification in M. roreri. A total of 88 polymorphic SNPs were used to evaluate the genetic diversity of 172 M. roreri cacao isolates resulting in 37 distinct genotypes (including 14 synonymous groups. Absence of heterozygosity for the 88 SNP markers indicates reproduction in M. roreri is clonal and likely due to a homothallic life style. The upper Magdalena Valley of Colombia showed the highest levels of genetic diversity with 20 distinct genotypes of which 13 were limited to this region, and indicates this region as the possible center of origin for M. roreri.

Combination of RNAseq and SNP nanofluidic array reveals the center of genetic diversity of cacao pathogen Moniliophthora roreri in the upper Magdalena Valley of Colombia and its clonality.

Science.gov (United States)

Ali, Shahin S; Shao, Jonathan; Strem, Mary D; Phillips-Mora, Wilberth; Zhang, Dapeng; Meinhardt, Lyndel W; Bailey, Bryan A

2015-01-01

Moniliophthora roreri is the fungal pathogen that causes frosty pod rot (FPR) disease of Theobroma cacao L., the source of chocolate. FPR occurs in most of the cacao producing countries in the Western Hemisphere, causing yield losses up to 80%. Genetic diversity within the FPR pathogen population may allow the population to adapt to changing environmental conditions and adapt to enhanced resistance in the host plant. The present study developed single nucleotide polymorphism (SNP) markers from RNASeq results for 13 M. roreri isolates and validated the markers for their ability to reveal genetic diversity in an international M. roreri collection. The SNP resources reported herein represent the first study of RNA sequencing (RNASeq)-derived SNP validation in M. roreri and demonstrates the utility of RNASeq as an approach for de novo SNP identification in M. roreri. A total of 88 polymorphic SNPs were used to evaluate the genetic diversity of 172 M. roreri cacao isolates resulting in 37 distinct genotypes (including 14 synonymous groups). Absence of heterozygosity for the 88 SNP markers indicates reproduction in M. roreri is clonal and likely due to a homothallic life style. The upper Magdalena Valley of Colombia showed the highest levels of genetic diversity with 20 distinct genotypes of which 13 were limited to this region, and indicates this region as the possible center of origin for M. roreri.

Balones que inspiran

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Daniel Samper Pizano

2005-09-01

Full Text Available Unión Magdalena "el fervor de un pueblo". Varios Autores. Universidad del Magdalena, Santa Marta, 2004, 271 págs., il. Rey de corazones. El Medellín, una pasión crónica. Varios autores. Medellín, Pregón Ediciones, 2004, 162 págs.

Factores de riesgo asociados a enfermedades cardiovasculares en un grupo de estudiantes del programa de medicina de la Universidad del Magdalena

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Dary Luz Mendoza Meza

2013-10-01

Full Text Available Las enfermedades del sistema cardiovascular se constituyen en la principal causa de morbilidad y mortalidad en la población adulta de los países occidentales, sin embargo en la actualidad la incidencia de estas patologías en la población menor de 30 años esta alcanzando niveles alarmantes. Una de las explicaciones a este fenómeno es el aumento de actitudes que promueven los llamados factores de riesgo modificables como el sedentarismo, los malos hábitos alimenticios, el tabaquismo y el alcoholismo; lo anterior sumado a factores no modificables como la herencia y el género, contribuyen al desencadenamiento de las enfermedades cardiovasculares. Adicionalmente estas patologías fueron consideradas casi exclusivas de los países industrializados, sin embargo el problema se está expandiendo a los países en vía de desarrollo como Colombia, esto quizá producto del aumento en el ritmo de vida y a la adquisición de estilos de vida poco saludables El objetivo de este estudio fue investigar la presencia de factores de riesgo asociados con las enfermedades cardiovasculares en un grupo de estudiantes del programa de medicina de la Universidad del Magdalena, como parte de un trabajo de investigación formativa correspondiente a la cátedra de Bioquímica. Como resultado se encontró que el 24% de los sujetos investigados presentó niveles de colesterol total y LDL por encima de los parámetros seguros. Los factores de riesgo asociados con estos altos niveles fueron, los antecedentes familiares con un 83%y el sobrepeso con un 50%. Este estudio se constituye en la base para la formulación de un proyecto que pretende determinar la relación entre los factores de riesgo cardiovascular modificable y no modificable, en la población estudiantil adolescente del Distrito de Santa Marta.

State of the a child labour

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Elvis Augusto Orozco Castillo

2012-12-01

Full Text Available The present article is a result of the first phase of the investigation titled ‘The Magdalena needs happy children’, in which the state of the art that is going to be investigated about the population of the minors that study and work in the district of Santa Marta and the Magdalena is shown.

Creating Sister Cities: An Exchange Across Hemispheres

Science.gov (United States)

Adams, M. T.; Cabezon, S. A.; Hardy, E.; Harrison, R. J.

2008-06-01

Sponsored by Associated Universities, Inc. (AUI) and the National Radio Astronomy Observatory (NRAO), this project creates a cultural and educational exchange program between communities in South and North America, linking San Pedro de Atacama in Chile and Magdalena, New Mexico in the United States. Both communities have similar demographics, are in relatively undeveloped regions of high-elevation desert, and are located near major international radio astronomy research facilities. The Atacama Large Millimeter/submillimeter Array (ALMA) is just 40 km east of San Pedro; the Very Large Array (VLA) is just 40 km west of Magdalena. In February 2007, the Mayor of San Pedro and two teachers visited Magdalena for two weeks; in July 2007 three teachers from Magdalena will visit San Pedro. These visits enable the communities to lay the foundation for a permanent, unique partnership. The teachers are sharing expertise and teaching methodologies for physics and astronomy. In addition to creating science education opportunities, this project offers students linguistic and cultural connections. The town of San Pedro, Chile, hosts nearly 100,000 tourists per year, and English language skills are highly valued by local students. Through exchanges enabled by email and distance conferencing, San Pedro and Magdalena students will improve English and Spanish language skills while teaching each other about science and their respective cultures. This poster describes the AUI/NRAO Sister Cities program, including the challenges of cross-cultural communication and the rewards of interpersonal exchanges between continents and cultures.

Estudo da Faciologia Orgânica em Rochas Geradoras Marinhas Cretáceas do Grupo Villeta, Bacia do Valle Superior del Magdalena,Colômbia: Implicações Paleoambientais.

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Juliana Andrade Iemini

2007-07-01

Full Text Available As rochas pelíticas do Cretáceo superior são asgeradoras da maior parte das reservas de petróleo naColômbia. A Bacia do Valle Superior Del Magdalena(VSM, possui afloramentos de boa qualidade e comníveis de evolução térmica, que variam desde oimaturo até o fim da “janela” de geração de óleo,tornando esta região como uma das mais propíciaspara o estudo dos processos de geração e migraçãoprimária do petróleo no Cretáceo superior. O objetivoprincipal consistiu no estudo de fácies orgânicaatravés da caracterização geoquímica e análisepalinofaciológica das rochas geradoras de petróleocretáceas do VSM numa seção aflorante (QuebradaBambuca, objetivando o entendimento do controleexercido pela evolução paleoambiental sobre opotencial gerador e as variações composicionaisda matéria orgânica a partir da integração dosdados geoquímicos e palinofaciológicos. Paratanto, foram utilizadas análises organogeoquímicas(Carbono Orgânico Total - COT e Pirólise Rock-Eval, cedidas pelo Instituto Colombiano doPetróleo ICP - ECOPETROL e palinofaciológicasem amostras das Formações Tetuan Bambuca e LaLuna, Grupo Villeta, Bacia do Valle Superior delMagdalena. As rochas dessa formação apresentamum elevado conteúdo orgânico, com valores deCOT de até 23%. As lâminas organopalinológicasforam analisadas através de técnicas de palinofácies(microscopia em luz branca transmitida e luz azul/ultravioleta incidente-fluorescência realizando acontagem dos componentes orgânicos particuladosdos grupos do querogênio (fitoclasto, palinomorfoe matéria orgânica amorfa e da determinação doÍndice de Coloração de Esporos (ICE. A analisemicroscópica revelou um predomínio de matériaorgânica amorfa. Os palinomorfos são representadospor dinoflagelados e esporomorfos, sendo osdinoflagelados os mais representativos. A matériaorgânica amorfa apresenta fluorescência amarelaa laranja. O material lenhoso representa a

Oceanographic features of the upwelling in front of Gaira's intent Magdalena Department, minor dry season of 2006

International Nuclear Information System (INIS)

Arevalo Martinez, Damian Leonardo; Franco Herrera, Andres

2008-01-01

In order to determine the influence of the ocean and atmospheric coupling dynamics on upwelling events, caused by Ekman's transport and by continental winds, as well as the possible fertilization effect produced by the increasing of the concentration of inorganic nutrients in coastal waters of Gaira's inlet, Magdalena department, during the minor dry season of 2006, atmospheric, oceanographic, and biological variables were measured by the implementation of the Eurelian method. A total of four samplings were carried out, among which two presented atmospheric and oceanic conditions that evidence the occurrence of upwelling events 48 hours before in response to strong winds coming from northeast (trade winds) and from the continent, whereas the other two samplings presented similar characteristics to those typical of rainy seasons. During this study, wind velocity and direction presented expected values during upwelling events caused by Ekman's transport and by continental winds (≥ 4.5 m/s, 26 Celsius degrade and 120 Celsius degrade, respectively), generating enough stress levels in the sea surface layer that could move it offshore (1.2 dynes/cm 2 ). During the days following the upwelling events, anomalies in water physical and chemical characteristics were observed, presenting low temperatures (26.1 +- 1.1 Celsius degrade), high salinity (36.0 +- 0.5) and, as a consequence, high densities (δ 26.31 +- 0.50), as well as low dissolved oxygen levels (4.04 +- 0.29 mL/L) and predominant subsaturation (84.3 +- 6.1 %) Inorganic nutrient concentration showed a relatively homogeneous behavior, keeping low nitrite (0.35 +- 0.02 μM) and phosphate (0.30 +- 0.01 μM) levels during all samplings, whereas the mean ammonium concentration was relatively high (1.08 +- 0.11 μM). In contrast, nitrate levels were high during all samplings, with higher values corresponding to upwelling events (9.48 +- 0.49 μM), although variation was not statistically significative. Phytoplanktonic

Fraccionamiento químico del carbono en suelos con diferentes usos en el departamento de Magdalena, Colombia

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José Rafael Vásquez Polo

2017-01-01

Full Text Available El cambio en el uso y manejo de los suelos puede afectar su capacidad para capturar carbono, cuya magnitud es desconocida en la región norte del departamento de Magdalena, Colombia. En este sentido es importante avanzar en estudios de caracterización cualitativa de la materia orgánica del suelo y el efecto que sobre ella han tenido los cambios de uso y el manejo de suelos dedicados al monocultivo. Por tal motivo se estudió la relación de los diferentes usos del suelo sobre los contenidos y formas de carbono (C en seis zonas edafoclimáticas al norte de dicho departamento, ubicado entre 5 y 956 m de altitud, con temperaturas medias entre 24 y 30 °C y precipitaciones medias entre 663 y 2000 mm. En cada zona se muestrearon dos sitios, cultivos tropicales y bosques (húmedos y secos tropicales, tomando cuatro muestras compuestas por diez submuestras aleatorias por sitio, en las que se determinó C total (Ct, C oxidable total (Cox, C hidrolizable con HCl 6N (Ch, C ligado al material húmico (Cp mediante extracción con pirofosfato sódico y C no oxidable (Cnox o C recalcitrante calculado como la diferencia Ct-Cox. El C total almacenado (Cta por unidad de superficie se calculó teniendo en cuenta la densidad aparente (Da y la profundidad de muestreo. Se encontraron diferencias estadísticas significativas por el efecto de las zonas, los valores mayores en porcentaje de Nt (0.32, Ct (3.90, Cox (3.85, Ch (2.05 y Cp (1.15 y Cta (109 Mg ha-1 se presentaron en los suelos de bosque húmedo de la zona 1, siendo la de mayor altitud (956 m, precipitación (2000 mm y menor temperatura (24 °C. Para el factor usos del suelo solo presentaron diferencias significativas las propiedades: Nt, Ct, Cox y Ch. Los valores de las formas de C más estables para los suelos de bosques, superaron los de los suelos de cultivo, en este contexto, Cox y Cnox representaron 95% y 5% del Ct, respectivamente Se observó en promedio una pérdida de 26% del Ct en suelos de

EFECTO DEL GLIFOSATO Y PARAQUAT SOBRE EL PROCESO DE NITRIFICACION EN UN SUELO DEL CORREGIMIENTO DE RÍO FRÍO (MAGDALENA, COLOMBIA

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Dary Luz Mendoza M.

2010-08-01

Full Text Available Se realizaron ensayos in vitro para determinar el efecto tóxico de los herbicidas glifosato y paraquat sobre el proceso de nitrificación con suelo franco-arenoso (pH: 5.8, Humedad: 13.05%, en el corregimiento de Río Frío, Magdalena. Los experimentos se realizaron por cuantificación espectrofométrica (método del ácido fenoldisulfonico de la concentración de nitratos producidos durante un periodo de incubación de 30 días usando el producto comercial y el principio activo grado estándard, en concentraciones de 10, 100 y 1000 ppm de cada uno de los herbicidas. Se observó una reducción de la nitrificación y un incremento en el período de retardo al aumentar las concentraciones, sin embargo, solamente con altas dosis del glifosato comercial (100 y 1000 ppm de Round Up y paraquat comercial (1000 ppm de Gramoxone hubo inhibición de la nitrificación. Tanto el paraquat comercial como el estándard, a 10 y 100 ppm, causaron leves disturbios sobre la nitrificación, la cual retornó a una normalidad relativa después de 30 días de incubación. Se observó una diferencia significativa (p<0,00\\ entre el glifosato comercial (concentración máxima de nitrato 55,0pg/g y el glifosato estándar (510,12pg/g NO3' a una concentración de 1000 ppm, lo cual sugiere que los adyuvantes en el producto comercial contribuyen a aumentar el efecto tóxico del herbicida. Estos datos son de interés para desarrollar técnicas de utilización de plaguicidas que permitan la recuperación de las poblaciones microbianas importantes para la fertilidad de los suelos.

Quelques aspects de l'évolution géomorphologique quaternaire du haut bassin du Magdalena (Colombie

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1974-01-01

Full Text Available Le bassin supérieur du Rio Magdalena présente les caractéristiques suivantes: 1 L'allure générale du bassin montre une influence très nette de la tectonique. Il s'est développé grâce à la présence d'un ensemble de blocs affaissés relativement les uns par rapport aux autres et entourés de blocs soulevés qui forment les limites du bassin. Dans certains cas des édifices volcaniques se superposent à ces blocs (Nevado del Huila, parâmes de Las Papas, par exemple. 2 Dans certaines parties du bassin (Nevado del Huila, environs du rio Mazamoras, et de Pitalito, le volcanisme s'est développé durant tout le Quaternaire. De la sorte, en général on observe un paroxysme volcanique durant la période d'accumulation de QIII dont la position géomorphologique est très semblable à celle du Mindel européen (Nebraska d'Amérique du Nord. Les matériaux volcaniques ont ainsi contribué à la mise en place de l'accumulation QIII mais cette accumulation n'est pas un produit du volcanisme car on l'observe bien développée dans des vallées ou il n'y eut aucune activité volcanique à cette époque. Ainsi, la mise en place du remplissage QIII provient d'une oscillation climatique. La granulométrie très grossière du matériel QIII provient de la combinaison du volcanisme et de cette oscillation climatique. 3 Dans le bassin, les événements morphogénétiques les plus saillants sont les suivants: - une incision extrêmement profonde des talwegs des avant l'accumulation de QIII cette incision a atteint au moins le niveau des rivières actuelles - la fossilisation des cañons incisés durant cette phase par l'accumulation très épaisse du matériel QIII. - postérieurement, a fonctionné une tendance très générale à l'incision uniquement interrompue par les phases d'accumulation QI et QII. Actuellement, cette incision n'est toujours pas arrivée au niveau atteint avant l'accumulation de QIII. Durant cette période d'incision des rivi

Functional Dissection of the DNA Interface of the Nucleotidyltransferase Domain of Chlorella Virus DNA Ligase*

Science.gov (United States)

Samai, Poulami; Shuman, Stewart

2011-01-01

Chlorella virus DNA ligase (ChVLig) has pluripotent biological activity and an intrinsic nick-sensing function. ChVLig consists of three structural modules that envelop nicked DNA as a C-shaped protein clamp: a nucleotidyltransferase (NTase) domain and an OB domain (these two are common to all DNA ligases) as well as a distinctive β-hairpin latch module. The NTase domain, which performs the chemical steps of ligation, binds the major groove flanking the nick and the minor groove on the 3′-OH side of the nick. Here we performed a structure-guided mutational analysis of the NTase domain, surveying the effects of 35 mutations in 19 residues on ChVLig activity in vivo and in vitro, including biochemical tests of the composite nick sealing reaction and of the three component steps of the ligation pathway (ligase adenylylation, DNA adenylylation, and phosphodiester synthesis). The results highlight (i) key contacts by Thr-84 and Lys-173 to the template DNA strand phosphates at the outer margins of the DNA ligase footprint; (ii) essential contacts of Ser-41, Arg-42, Met-83, and Phe-75 with the 3′-OH strand at the nick; (iii) Arg-176 phosphate contacts at the nick and with ATP during ligase adenylylation; (iv) the role of Phe-44 in forming the protein clamp around the nicked DNA substrate; and (v) the importance of adenine-binding residue Phe-98 in all three steps of ligation. Kinetic analysis of single-turnover nick sealing by ChVLig-AMP underscored the importance of Phe-75-mediated distortion of the nick 3′-OH nucleoside in the catalysis of DNA 5′-adenylylation (step 2) and phosphodiester synthesis (step 3). Induced fit of the nicked DNA into a distorted conformation when bound within the ligase clamp may account for the nick-sensing capacity of ChVLig. PMID:21335605

Functional dissection of the DNA interface of the nucleotidyltransferase domain of chlorella virus DNA ligase.

Science.gov (United States)

Samai, Poulami; Shuman, Stewart

2011-04-15

Chlorella virus DNA ligase (ChVLig) has pluripotent biological activity and an intrinsic nick-sensing function. ChVLig consists of three structural modules that envelop nicked DNA as a C-shaped protein clamp: a nucleotidyltransferase (NTase) domain and an OB domain (these two are common to all DNA ligases) as well as a distinctive β-hairpin latch module. The NTase domain, which performs the chemical steps of ligation, binds the major groove flanking the nick and the minor groove on the 3'-OH side of the nick. Here we performed a structure-guided mutational analysis of the NTase domain, surveying the effects of 35 mutations in 19 residues on ChVLig activity in vivo and in vitro, including biochemical tests of the composite nick sealing reaction and of the three component steps of the ligation pathway (ligase adenylylation, DNA adenylylation, and phosphodiester synthesis). The results highlight (i) key contacts by Thr-84 and Lys-173 to the template DNA strand phosphates at the outer margins of the DNA ligase footprint; (ii) essential contacts of Ser-41, Arg-42, Met-83, and Phe-75 with the 3'-OH strand at the nick; (iii) Arg-176 phosphate contacts at the nick and with ATP during ligase adenylylation; (iv) the role of Phe-44 in forming the protein clamp around the nicked DNA substrate; and (v) the importance of adenine-binding residue Phe-98 in all three steps of ligation. Kinetic analysis of single-turnover nick sealing by ChVLig-AMP underscored the importance of Phe-75-mediated distortion of the nick 3'-OH nucleoside in the catalysis of DNA 5'-adenylylation (step 2) and phosphodiester synthesis (step 3). Induced fit of the nicked DNA into a distorted conformation when bound within the ligase clamp may account for the nick-sensing capacity of ChVLig.

Book Reviews / Nick Ellison

Index Scriptorium Estoniae

Ellison, Nick

2008-01-01

J. Aidukaite doktoridissertatsiooni, The Emergence of the Post-Socialist Welfare State - The Case of the Baltic States: Estonia, Latvia and Lithuania, milles võrreldakse nende riikide sotsiaalpoliitikat ja sotsiaalabi süsteeme, refereering

ERC rewards CERN researchers

CERN Multimedia

Rosaria Marraffino

2015-01-01

The European Research Council (ERC) has awarded starting grants to Magdalena Kowalska, a member of the ISOLDE physics team, and Claude Duhr, CERN Theory Division. The funding will enable them to build their own research teams at CERN, engaging postdocs and PhD students.   Magdalena Kowalska and Claude Duhr. The ERC fosters scientific excellence in Europe through competitive funding. Its grants are awarded to projects headed by researchers – both beginning-of-career and established – via an open, peer-reviewed competition. In December 2014, Magdalena Kowalska and Claude Duhr were awarded grants to pursue research in ultra-sensitive nuclear magnetic resonance (NMR) in liquids and mathematical structures in scattering amplitudes, respectively. “Our research project aims to apply an ultra-sensitive NMR technique using radioisotopes to liquids in order to study the interaction of metal ions with biological molecules such as proteins, DNA or RNA,” says Mag...

Efectos de la concentración de glucosa sobre la activación de la movilidad espermática en bocachico Prochilodus magdalenae (Pisces, Characiformes

Directory of Open Access Journals (Sweden)

Gregorio Martínez

2011-05-01

Full Text Available Objetivo. Determinar la concentración de glucosa no activadora de la movilidad espermática como componente de futuros diluyentes para la crioconservación de semen de Prochilodus magdalenae. Materiales y métodos. Se analizó semen inactivo de tres machos obtenido por inducción hormonal. Una submuestra de 0.25 µL de semen de cada macho fue depositada sobre una cámara de Makler y evaluada usando diferentes concentraciones de glucosa (0, 1, 2, 3, 4, 5, 6, 7, 8, 9 y 10% mediante la adición de 75 µL de cada solución sobre el semen (n=3. De cada solución se determinó la osmolaridad, siendo de 0, 62, 124, 186, 250, 310, 360, 410, 472, 536 y 620 mOsm/kg, respectivamente, así como la del plasma seminal (~250���300 mOsm/kg. Mediante el software Sperm Class Analyzer (SCA se determinó la velocidad curvilínea (VCL y recta (VSL (µm/seg, movilidad rápida (%, media (% y lenta (% y el porcentaje de espermatozoides inmóviles. El tiempo de activación de la movilidad (segundos se obtuvo por cronometraje y visualización bajo microscopio de campo claro. Resultados. Concentraciones de 0 a 5% de glucosa produjeron activación de la movilidad espermática, no obstante, a partir de glucosa al 6% no fue detectada activación visual; sin embargo, SCA detectó movilidad total (7.2%, VCL (5.1 µm/seg y VSL (1.7 µm/seg. A partir de glucosa al 7% el SCA detectó 100% de inmovilidad, no adquirida posteriormente con agua destilada. Conclusiones. La concentración de glucosa tiene efecto determinante en la viabilidad y activación de semen fresco. Una concentración de 6% de glucosa puede ser utilizada como solución no activadora de la movilidad espermática en futuros diluyentes para la crioconservación de semen de esta especie.

Proměna české kuchyně z dob Magdaleny Dobromily Rettigové po současné trendy zdravého životního stylu

OpenAIRE

Málková, Simona

2018-01-01

The thesis focuses on the topic of changing eating habits from the time of Magdalena Dobromila Rettigová to contemporary trends of healthy lifestyle. It is a gradual development of eating from the late 18th century to the present. In the thereoretic part there is a discription of the contrast of the Czech writer's life and a present-day lifestyle. We can find a chapter about a life of Magdalena Dobromila Rettigová, background information leading up to, writing the cook book "Cooking at home",...

Evaluación de procedencias de Pinus greggii Engelm. ex Parl. en plantaciones de la Mixteca Oaxaqueña

OpenAIRE

Velasco-Velasco, Vicente Arturo; Enríquez-del Valle, José Raymundo; Rodríguez-Ortiz, Gerardo; Campos-Ángeles, Gisela Virginia; Gómez-Cárdenas, Martín; García-García, María Luisa

2012-01-01

Se evaluaron 13 procedencias de Pinus greggii en dos plantaciones de siete años de edad establecidas en 1997 en Tlacotepec Plumas y Magdalena Zahuatlán en la Mixteca de Oaxaca. El diseño experimental fue de bloques completos aleatorios con arreglo factorial 2 x 13 (localidades por procedencias). La unidad experimental constó de nueve individuos y se tuvieron 12 repeticiones. Los árboles de las 13 procedencias ubicados en Tlacotepec Plumas y Magdalena Zahuatlán tuvieron en promedio 4.7 y 3.7 m...

Combined use of local and global hydrometeorological data with regional and global hydrological models in the Magdalena - Cauca river basin, Colombia

Science.gov (United States)

Rodriguez, Erasmo; Sanchez, Ines; Duque, Nicolas; Lopez, Patricia; Kaune, Alexander; Werner, Micha; Arboleda, Pedro

2017-04-01

The Magdalena Cauca Macrobasin (MCMB) in Colombia, with an area of about 257,000 km2, is the largest and most important water resources system in the country. With almost 80% of the Colombian population (46 million people) settled in the basin, it is the main source of water for demands including human consumption, agriculture, hydropower generation, industrial activities and ecosystems. Despite its importance, the basin has witnessed enormous changes in land-cover and extensive deforestation during the last three decades. To make things more complicated, the MCMB currently lacks a set of tools to support planning and decision making processes at scale of the whole watershed. Considering this, the MCMB has been selected as one of the six different regional case studies in the eartH2Observe research project, in which hydrological and meteorological reanalysis products are being validated for the period 1980-2012. The combined use of the hydrological and meteorological reanalysis data, with local hydrometeorological data (precipitation, temperature and streamflow) provided by the National Hydrometeorological Agency (IDEAM), has given us the opportunity to implement and test three hydrological models (VIC, WFLOW and a Water Balance Model based on the Budyko framework) at the basin scale. Additionally, results from the global models in the eartH2Observe hydrological reanalysis have been used to evaluate their performance against the observed streamflow data. This paper discusses the comparison between streamflow observations and simulations from the global hydrological models forced with the WFDEI data, and regional models forced with a combination of observed and meteorological reanalysis data, in the whole domain of the MCMB. For the three regional models analysed results show good performances for some sub-basins and poor performances for others. This can be due to the smoothing of the precipitation fields, interpolated from point daily rainfall data, the effect of

Variabilidad espacial de propiedades físicas y químicas en suelos de la granja experimental de la Universidad del Magdalena (Santa Marta, Colombia

Directory of Open Access Journals (Sweden)

José Rafael Vásquez Polo

2010-10-01

Full Text Available En suelos destinados a la actividad agrícola de la granja experimental de la Universidad del Magdalena, Santa Marta - Colombia (74° 07' Y 74° 12' longitud oeste y 11° 11' y 11° 15' latitud, se estudió la variabilidad espacial de las propiedades físicas: humedad gravimétrica, textura, densidad aparente (Da, resistencia a la penetración (RP y conductividad hidráulica (KH y químicas: Materia orgánica (MO, pH, CIC, N, P, K, Ca+2, Mg+2, Na+, Cu, Zn, Fe, Mn, B, utilizando una grid o malla de 29m*29m con 184 puntos de muestreo. El análisis de los datos se realizó empleando enfoques univariado, geoestadístico y multivariado. El análisis estadístico de los resultados mostró gran variabilidad de las propiedades; la densidad aparente y el pH presentaron baja variación; los componentes texturales, la RP, Ca+2, Mg+2, Cu y B mostraron variación media, mientras que la humedad gravimétrica, KH, MO, K+, Na+, CIC, Zn, P, Mn y Fe, expresaron alta variación. Con el análisis Geoestadístico se visualizó y graficó la variabilidad de cada propiedad evaluada y se evidenció la existencia de moderada dependencia espacial en las propiedades excepto en los contenidos de arena, Ca+2, CIC, P y Cu que revelan fuerte dependencia espacial. El índice de homogeneidad multivariado para los suelos en estudio fue bajo, siendo textura, Na+, B, Fe, K, pH, Ca y MO las propiedades con mayor contribución a la heterogeneidad. El análisis de clasificación permitió agrupar cuatro zonas con condiciones edáficas similares, las que se podrán utilizar para determinar el sistema de muestreo, número de muestras, tamaño y distribución de unidades experimentales y con ello facilitar la implementación de prácticas de manejo agronómico acordes con las características de cada zona.

Variabilidad espacial de propiedades físicas y químicas en suelos de la granja experimental de la Universidad del Magdalena (Santa Marta, Colombia

Directory of Open Access Journals (Sweden)

Menjivar Flores Juan Carlos

2010-12-01

Full Text Available En suelos destinados a la actividad agrícola de la granja experimental de la Universidad del Magdalena, Santa Marta - Colombia (74º 07' Y 74º 12' longitud oeste y 11º 11' y 11º 15' latitud, se estudió la variabilidad espacial de las propiedades físicas: humedad gravimétrica, textura, densidad aparente (Da, resistencia a la penetración (RP y conductividad hidráulica (KH y químicas: Materia orgánica (MO, pH, CIC, N, P, K, Ca+2, Mg+2, Na+, Cu, Zn, Fe, Mn, B, utilizando una grid o malla de 29m*29m con 184 puntos de muestreo. El análisis de los datos se realizó empleando enfoques univariado, geoestadístico y multivariado. El análisis estadístico de los resultados mostró gran variabilidad de las propiedades; la densidad aparente y el pH presentaron baja variación; los componentes texturales, la RP, Ca+2, Mg+2, Cu y B mostraron variación media, mientras que la humedad gravimétrica, KH, MO, K+, Na+, CIC, Zn, P, Mn y Fe, expresaron alta variación. Con el análisis Geoestadístico se visualizó y graficó la variabilidad de cada propiedad evaluada y se evidenció la existencia de moderada dependencia espacial en las propiedades excepto en los contenidos de arena, Ca+2, CIC, P y Cu que revelan fuerte dependencia espacial. El índice de homogeneidad multivariado para los suelos en estudio fue bajo, siendo textura, Na+, B, Fe, K, pH, Ca y MO las propiedades con mayor contribución a la heterogeneidad. El análisis de clasificación permitió agrupar cuatro zonas con condiciones edáficas similares, las que se podrán utilizar para determinar el sistema de muestreo, número de muestras, tamaño y distribución de unidades experimentales y con ello facilitar la implementación de prácticas de manejo agronómico acordes con las características de cada zona.

El ambiente marino costero de la Región La Libertad 2010

OpenAIRE

Rebaza Castillo, Víctor; Tresierra, Alvaro; Alfaro Mudarra, Santos; Vásquez Ruiz, Cinthia

2013-01-01

Entre el 26 abril y 10 mayo 2010, se efectuó la evaluación del ambiente costero de la región La Libertad, determinándose que Huanchaco, Salaverry y Magdalena de Cao tienen el más alto grado de contaminación marina. Salaverry y Magdalena de Cao presentan los valores más bajos de diversidad bentónica y de volumen de plancton. Las variables oceanográficas primarias se debieron a procesos de surgencia recientes. La concentración de sólidos suspendidos totales de Chérrepe cumplió con los Estándare...

Once a physicist: Nick Horvath

Science.gov (United States)

Horvath, Nick

2009-05-01

How did you get into basketball? That's an easy one: I was tall. I had zero interest in sport at a young age and was pushed into basketball against my will when I was about eight. I hated it. The next year, however, I was so much taller than everyone else that in my first game, even though I had no idea what I was doing, I could simply hold the ball above my head and shoot over everyone. I fell in love with basketball after that first game. If I had played badly that day, it is likely that I would not have played again in my entire life.

Structure-function analysis of the OB and latch domains of chlorella virus DNA ligase.

Science.gov (United States)

Samai, Poulami; Shuman, Stewart

2011-06-24

Chlorella virus DNA ligase (ChVLig) is a minimized eukaryal ATP-dependent DNA sealing enzyme with an intrinsic nick-sensing function. ChVLig consists of three structural domains, nucleotidyltransferase (NTase), OB-fold, and latch, that envelop the nicked DNA as a C-shaped protein clamp. The OB domain engages the DNA minor groove on the face of the duplex behind the nick, and it makes contacts to amino acids in the NTase domain surrounding the ligase active site. The latch module occupies the DNA major groove flanking the nick. Residues at the tip of the latch contact the NTase domain to close the ligase clamp. Here we performed a structure-guided mutational analysis of the OB and latch domains. Alanine scanning defined seven individual amino acids as essential in vivo (Lys-274, Arg-285, Phe-286, and Val-288 in the OB domain; Asn-214, Phe-215, and Tyr-217 in the latch), after which structure-activity relations were clarified by conservative substitutions. Biochemical tests of the composite nick sealing reaction and of each of the three chemical steps of the ligation pathway highlighted the importance of Arg-285 and Phe-286 in the catalysis of the DNA adenylylation and phosphodiester synthesis reactions. Phe-286 interacts with the nick 5'-phosphate nucleotide and the 3'-OH base pair and distorts the DNA helical conformation at the nick. Arg-285 is a key component of the OB-NTase interface, where it forms a salt bridge to the essential Asp-29 side chain, which is imputed to coordinate divalent metal catalysts during the nick sealing steps.

Structure-Function Analysis of the OB and Latch Domains of Chlorella Virus DNA Ligase*

Science.gov (United States)

Samai, Poulami; Shuman, Stewart

2011-01-01

Chlorella virus DNA ligase (ChVLig) is a minimized eukaryal ATP-dependent DNA sealing enzyme with an intrinsic nick-sensing function. ChVLig consists of three structural domains, nucleotidyltransferase (NTase), OB-fold, and latch, that envelop the nicked DNA as a C-shaped protein clamp. The OB domain engages the DNA minor groove on the face of the duplex behind the nick, and it makes contacts to amino acids in the NTase domain surrounding the ligase active site. The latch module occupies the DNA major groove flanking the nick. Residues at the tip of the latch contact the NTase domain to close the ligase clamp. Here we performed a structure-guided mutational analysis of the OB and latch domains. Alanine scanning defined seven individual amino acids as essential in vivo (Lys-274, Arg-285, Phe-286, and Val-288 in the OB domain; Asn-214, Phe-215, and Tyr-217 in the latch), after which structure-activity relations were clarified by conservative substitutions. Biochemical tests of the composite nick sealing reaction and of each of the three chemical steps of the ligation pathway highlighted the importance of Arg-285 and Phe-286 in the catalysis of the DNA adenylylation and phosphodiester synthesis reactions. Phe-286 interacts with the nick 5′-phosphate nucleotide and the 3′-OH base pair and distorts the DNA helical conformation at the nick. Arg-285 is a key component of the OB-NTase interface, where it forms a salt bridge to the essential Asp-29 side chain, which is imputed to coordinate divalent metal catalysts during the nick sealing steps. PMID:21527793

C-terminal phenylalanine of bacteriophage T7 single-stranded DNA-binding protein is essential for strand displacement synthesis by T7 DNA polymerase at a nick in DNA.

Science.gov (United States)

Ghosh, Sharmistha; Marintcheva, Boriana; Takahashi, Masateru; Richardson, Charles C

2009-10-30

Single-stranded DNA-binding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replication. Not only does it remove impediments of secondary structure in the DNA, it also modulates the activities of the other replication proteins. The acidic C-terminal tail of gp2.5, bearing a C-terminal phenylalanine, physically and functionally interacts with the helicase and DNA polymerase. Deletion of the phenylalanine or substitution with a nonaromatic amino acid gives rise to a dominant lethal phenotype, and the altered gp2.5 has reduced affinity for T7 DNA polymerase. Suppressors of the dominant lethal phenotype have led to the identification of mutations in gene 5 that encodes the T7 DNA polymerase. The altered residues in the polymerase are solvent-exposed and lie in regions that are adjacent to the bound DNA. gp2.5 lacking the C-terminal phenylalanine has a lower affinity for gp5-thioredoxin relative to the wild-type gp2.5, and this affinity is partially restored by the suppressor mutations in DNA polymerase. gp2.5 enables T7 DNA polymerase to catalyze strand displacement DNA synthesis at a nick in DNA. The resulting 5'-single-stranded DNA tail provides a loading site for T7 DNA helicase. gp2.5 lacking the C-terminal phenylalanine does not support this event with wild-type DNA polymerase but does to a limited extent with T7 DNA polymerase harboring the suppressor mutations.

C-terminal Phenylalanine of Bacteriophage T7 Single-stranded DNA-binding Protein Is Essential for Strand Displacement Synthesis by T7 DNA Polymerase at a Nick in DNA*

Science.gov (United States)

Ghosh, Sharmistha; Marintcheva, Boriana; Takahashi, Masateru; Richardson, Charles C.

2009-01-01

Single-stranded DNA-binding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replication. Not only does it remove impediments of secondary structure in the DNA, it also modulates the activities of the other replication proteins. The acidic C-terminal tail of gp2.5, bearing a C-terminal phenylalanine, physically and functionally interacts with the helicase and DNA polymerase. Deletion of the phenylalanine or substitution with a nonaromatic amino acid gives rise to a dominant lethal phenotype, and the altered gp2.5 has reduced affinity for T7 DNA polymerase. Suppressors of the dominant lethal phenotype have led to the identification of mutations in gene 5 that encodes the T7 DNA polymerase. The altered residues in the polymerase are solvent-exposed and lie in regions that are adjacent to the bound DNA. gp2.5 lacking the C-terminal phenylalanine has a lower affinity for gp5-thioredoxin relative to the wild-type gp2.5, and this affinity is partially restored by the suppressor mutations in DNA polymerase. gp2.5 enables T7 DNA polymerase to catalyze strand displacement DNA synthesis at a nick in DNA. The resulting 5′-single-stranded DNA tail provides a loading site for T7 DNA helicase. gp2.5 lacking the C-terminal phenylalanine does not support this event with wild-type DNA polymerase but does to a limited extent with T7 DNA polymerase harboring the suppressor mutations. PMID:19726688

Distribución de los vectores de Leishmania infantum (Kinetoplastida: Trypanosomatidae en Colombia

Directory of Open Access Journals (Sweden)

Camila González

2006-10-01

Full Text Available Introducción. Debido a la importancia que tiene la vigilancia entomológica como principalmedida de control en el manejo de la leishmaniasis visceral, es necesario contar con informaciónactualizada acerca de la distribución y ecología de los insectos involucrados en la transmisiónpara optimizar las estrategias de prevención. Objetivo. Presentar la distribución actualizada geo-referenciada de L. longipalpis y L. evansi,vectores de los parásitos que causan leishmaniasis visceral en Colombia, teniendo en cuentala asociación de los insectos con su hábitat. Materiales y métodos. Los registros de distribución se obtuvieron a partir de los ejemplaresrecolectados en Colombia desde 1967. La información obtenida se organizó en una base dedatos a partir de la cual se tomaron las localidades que, posteriormente, fueron sometidas aanálisis geográficos por medio de Arc View que se utilizaron para realizar los mapas dedistribución. Resultados. Para L. longipalpis se obtuvieron 40 localidades todas distribuidas a lo largo delvalle del río Magdalena: Alto (24, Medio (11 y Bajo (5 Magdalena. L. evansi fue registradoen 19 localidades también ubicadas en el mismo valle: cinco en el Magdalena Medio y 14 elMagdalena Bajo. Conclusiones. Ambas especies demostraron una consistente asociación con regionesclasificadas principalmente como bosque seco tropical según las zonas de vida de Holdridgelo que confirma el riesgo epidemiológico de leishmaniasis visceral en estas áreas.Palabras clave: Psychodidae, leishmaniasis visceral, Colombia.

Two new trans-Andean species of Imparfinis Eigenmann & Norris, 1900 (Siluriformes: Heptapteridae from Colombia

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Armando Ortega-Lara

Full Text Available Two new species of Imparfinis are described from the trans-Andean region of Colombia. Imparfinis timana is diagnosed by having longer anal fin base (12.4-15.5% in SL, in combination with long adipose fin (24.6-31.3% in SL, 5-6 gill rakers on the first ceratobranchial, 42-43 vertebrae and additional measurements. Imparfinis usmai is distinguished by the combination of first ray of dorsal fin longest, but not projected as a long filament, long adipose fin (21.1-27.0% in SL, maxillary barbel exceeding pelvic-fin base, 39-40 vertebrae, upper caudal-fin lobe pointed and longer than lower lobe, lower lobe rounded, 7-8 gill rakers on the first ceratobranchial, as well as additional measurements. Imparfinis timana is only known from río Guarapas, a small tributary of the upper course of the río Magdalena. Imparfinis usmai is broadly distributed in the upper basin of ríos Cauca and Magdalena, and in the lower Patía river basin. The restricted distribution of I. nemacheir to trans-Andean drainages (Atrato, Magdalena, and Lago de Maracaibo is also discussed.

Site-specifically modified oligodeoxyribonucleotides as templates for Escherichia coli DNA polymerase I

International Nuclear Information System (INIS)

O'Connor, D.; Stoehrer, G.

1985-01-01

Oligodeoxyribonucleotides with site-specific modifications have been used as substrates for Escherichia coli DNA polymerase I holoenzyme and Klenow fragment. Modifications included the bulky guanine-8-aminofluorene adduct and a guanine oxidation product resembling the product of photosensitized DNA oxidation. By a combination of primers and nick-mers, conditions of single-strand-directed DNA synthesis and nick-translation could be created. The results show that the polymerase can bypass both types of lesions. Bypass occurs on a single-stranded template but is facilitated on a nicked, double-stranded template. Only purines, with guanine more favored than adenine, are incorporated across both lesions. The results indicate that site-specifically modified oligonucleotides can be sensitive probes for the action of polymerases on damaged templates. They also suggest a function for polymerase I, in its nick-translation capacity, during DNA repair and mutagenesis

Alteration of Sequence Specificity of the Type IIS Restriction Endonuclease BtsI

OpenAIRE

Guan, Shengxi; Blanchard, Aine; Zhang, Penghua; Zhu, Zhenyu

2010-01-01

The Type IIS restriction endonuclease BtsI recognizes and digests at GCAGTG(2/0). It comprises two subunits: BtsIA and BtsIB. The BtsIB subunit contains the recognition domain, one catalytic domain for bottom strand nicking and part of the catalytic domain for the top strand nicking. BtsIA has the rest of the catalytic domain that is responsible for the DNA top strand nicking. BtsIA alone has no activity unless it mixes with BtsIB to reconstitute the BtsI activity. During characterization of ...

Niveles de pensamiento y resolución de problemas matemáticos en los estudiantes del programa psicología de una universidad pública de Santa Marta (Magdalena

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Liliana Quintero Díaz

2013-10-01

Full Text Available Title: Levels of thinking and solving mathematical problems students program in psychology of a public university Santa Marta (Magdalena.ResumenEl objetivo de la presente investigación fue determinar las diferencias entre los niveles de pensamiento lógico y la resolución de problemas matemáticos de los estudiantes de primero a octavo semestre del programa Psicología de una Universidad Pública, a través de un tipo de estudio descriptivo-correlacional, aplicando instrumentos que miden el nivel de pensamiento lógico (TOLT, y la resolución de problemas matemáticos (PRP. Se encontró a nivel general un 56.7% en pensamiento concreto, un 37.4% en transición y el 5.8% en formal; en cuanto a resolución de problemas matemáticos, el 50.9% se encuentra en un nivel bajo, 19.9% en medio, y 29.2% en alto. Los resultados señalan la existencia de diferencias entre los niveles de pensamiento y resolución de problemas matemáticos en los estudiantes de primero a octavo semestre del programa de Psicología de una Universidad Pública a una t=-4.676, significancia bilateral de 0.00. (DUAZARY 2012 No. 2, 123 - 131AbstractThe objective of this research was to determine the differences between the levels of logical thinking and solving mathematical problems of students from first to eighth semester of the program psychology of a public University, through a kind of descriptivo-correlacional study, using instruments that measure the level of logical thinking (TOLT, and mathematical problem solving (PRP. Was found at a general level a 56.7% in particular thinking, a 37.4 per cent in transition, and 5.8% in formal; in mathematical problem solving, the 50.9% is located in a low level, 19.9% in the Middle, and 29.2% high. The results indicate the existence of differences between the levels of thinking and mathematical problem solving in students from first to eighth semester of the programme of psychology of a public University, with a t =-4.676 to bilateral

A conserved helicase processivity factor is needed for conjugation and replication of an integrative and conjugative element.

Directory of Open Access Journals (Sweden)

Jacob Thomas

Full Text Available Integrative and conjugative elements (ICEs are agents of horizontal gene transfer and have major roles in evolution and acquisition of new traits, including antibiotic resistances. ICEs are found integrated in a host chromosome and can excise and transfer to recipient bacteria via conjugation. Conjugation involves nicking of the ICE origin of transfer (oriT by the ICE-encoded relaxase and transfer of the nicked single strand of ICE DNA. For ICEBs1 of Bacillus subtilis, nicking of oriT by the ICEBs1 relaxase NicK also initiates rolling circle replication. This autonomous replication of ICEBs1 is critical for stability of the excised element in growing cells. We found a conserved and previously uncharacterized ICE gene that is required for conjugation and replication of ICEBs1. Our results indicate that this gene, helP (formerly ydcP, encodes a helicase processivity factor that enables the host-encoded helicase PcrA to unwind the double-stranded ICEBs1 DNA. HelP was required for both conjugation and replication of ICEBs1, and HelP and NicK were the only ICEBs1 proteins needed for replication from ICEBs1 oriT. Using chromatin immunoprecipitation, we measured association of HelP, NicK, PcrA, and the host-encoded single-strand DNA binding protein Ssb with ICEBs1. We found that NicK was required for association of HelP and PcrA with ICEBs1 DNA. HelP was required for association of PcrA and Ssb with ICEBs1 regions distal, but not proximal, to oriT, indicating that PcrA needs HelP to progress beyond nicked oriT and unwind ICEBs1. In vitro, HelP directly stimulated the helicase activity of the PcrA homologue UvrD. Our findings demonstrate that HelP is a helicase processivity factor needed for efficient unwinding of ICEBs1 for conjugation and replication. Homologues of HelP and PcrA-type helicases are encoded on many known and putative ICEs. We propose that these factors are essential for ICE conjugation, replication, and genetic stability.

Genesis / Juta Kivimäe

Index Scriptorium Estoniae

Kivimäe, Juta, 1952-

2003-01-01

Poola skulptori Magdalena Schmidt-G̤ra installatsioonid "Genesis I, II" rahvusraamatukogu fuajees ja peanäitusesaalis, kus oli samal ajal eksponeeritud eesti nahakunstnike Luule Maari ja Ruuda Maarandi köitenäitus

Department of Ecotourism and Wildlife Management, Federal ...

African Journals Online (AJOL)

USER

2014-09-09

Sep 9, 2014 ... development in Olumirin Waterfall Southwestern Nigeria. A field survey via .... waterfall is located. Source: Osun State Ministry of Land and Physical planning and Urban Development (2012) .... Magdalena, Mexico. Ocean & ...

Saaremaa / Raul Salumäe

Index Scriptorium Estoniae

Salumäe, Raul

2003-01-01

Kuressaare linnuse keldrisaalis poola kunstniku Magdalena Schmidt-Gora näitus-installatsioon "Genesis I, II", 3. korruse väikeses saalis läti fotograafi Astra Tomsone fotonäitus "Loojang minu akna taga"

Adenosine triphosphate stimulates Aquifex aeolicus MutL endonuclease activity.

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Jerome Mauris

2009-09-01

Full Text Available Human PMS2 (hPMS2 homologues act to nick 5' and 3' to misincorporated nucleotides during mismatch repair in organisms that lack MutH. Mn(++ was previously found to stimulate the endonuclease activity of these homologues. ATP was required for the nicking activity of hPMS2 and yPMS1, but was reported to inhibit bacterial MutL proteins from Thermus thermophilus and Aquifex aeolicus that displayed homology to hPMS2. Mutational analysis has identified the DQHA(X(2E(X(4E motif present in the C-terminus of PMS2 homologues as important for endonuclease activity.We examined the effect ATP had on the Mn(++ induced nicking of supercoiled pBR322 by full-length and mutant A. aeolicus MutL (Aae MutL proteins. Assays were single time point, enzyme titration experiments or reaction time courses. The maximum velocity for MutL nicking was determined to be 1.6+/-0.08x10(-5 s(-1 and 4.2+/-0.3x10(-5 s(-1 in the absence and presence of ATP, respectively. AMPPNP stimulated the nicking activity to a similar extent as ATP. A truncated Aae MutL protein composed of only the C-terminal 123 amino acid residues was found to nick supercoiled DNA. Furthermore, mutations in the conserved C-terminal DQHA(X(2E(X(4E and CPHGRP motifs were shown to abolish Aae MutL endonuclease activity.ATP stimulated the Mn(++ induced endonuclease activity of Aae MutL. Experiments utilizing AMPPNP implied that the stimulation did not require ATP hydrolysis. A mutation in the DQHA(X(2E(X(4E motif of Aae MutL further supported the role of this region in endonclease activity. For the first time, to our knowledge, we demonstrate that changing the histidine residue in the conserved CPHGRP motif abolishes endonucleolytic activity of a hPMS2 homologue. Finally, the C-terminal 123 amino acid residues of Aae MutL were sufficient to display Mn(++ induced nicking activity.

Characterization of linear forms of the circular enterocin AS-48 obtained by limited proteolysis

NARCIS (Netherlands)

Montalbán-López, Manuel; Spolaore, Barbara; Pinato, Odra; Martínez-Bueno, Manuel; Valdivia, Eva; Maqueda, Mercedes; Fontana, Angelo

2008-01-01

AS-48 is a 70-residue circular peptide from Enterococcus faecalis with a broad antibacterial activity. Here, we produced by limited proteolysis a protein species carrying a single nicking and fragments of 55 and 38 residues. Nicked AS-48 showed a lower helicity by far-ultraviolet circular dichroism

Sudha Bhattacharya Jawaharlal Nehru University New Delhi

Indian Academy of Sciences (India)

TSC

Bottom strand. 1. 2. 3. G A T C. 5. 1. 3. 4. 2. *. Nicking profile of EN on the 174 bp substrate. Insertion point. Bottom strand labelled 174bp substrate. Transposon insertion point. Endonuclease nicks at three hot spots. The transposon inserts at hot spot # 3 ...

Variabilidad de la presión arterial pre y post-quirúrgica en pacientes sometidos a cirugía oral que asistieron a la clínica odontológica de la Universidad del Magdalena en los meses de abril y mayo periodo 2008

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Luis Castillo Castilla

2013-10-01

Full Text Available Resumen La incidencia de enfermedades cardiovasculares se ha asociado como una de las principales causas de mortalidad no violenta en Colombia. La hipertensión arterial (HTA es la patología cardiovascular de mayor prevalencia en nuestra nación, con un rango que oscila entre 13% y 23%, por la alta frecuencia en la población adulta. Los últimos avances en el estudio de la HTA revelan que el componente genético determina el 50% de los niveles altos de presión arterial (PA y el 50% restante es determinado por la presencia de una serie de factores de riesgos exógenos1. Los valores de PA en el ejercicio de la practica odontológica pueden alterarse, lo que es importante tener en cuenta al momento de ejecutar procedimientos quirúrgicos ya que puede estar sujeta a diversos factores de riesgo tales como estrés, alcoholismo, tabaquismo, consumo de medicamentos y sedentarismo, entre otros, agentes que pueden causar su aumento o disminución, constituyendo en su conjunto un serio riesgo para la vida del paciente sino existe su adecuada atención ante cualquier práctica quirúrgico1,2. Con el objeto de determinar la variabilidad de la presión arterial generada en los procedimientos pre y post-quirúrgicos, se tomó una muestra de 33 pacientes en la Clínica Odontológica de la Universidad del Magdalena a los cuales se les realizó una toma de presión arterial en cada una de las fases del evento correspondiente. Los datos se tabularon y analizaron mediante estadísticas descriptivas; obteniéndose que el 64% de los pacientes presentó aumento de la presión arterial tras el procedimiento quirúrgico, el 18% mantuvo la presión normal y el restante manifestó descenso de la misma. (DUAZARY 2011, 48 - 57 AbstractThe incidence of cardiovascular disease has been associated as the main cause of death does not violent in Colombia. Hypertension (HT is the highest prevalence of cardiovascular disease in our nation, with a range of between 13% and 23

FAMILY NEMESTRINIDAE.

Science.gov (United States)

Kosmann, Cecília; Lamas, Carlos José Einicker

2016-06-14

Nemestrinidae (Diptera, Nemestrinoidea) are a relatively rare group of flies, and the knowledge on the Colombian fauna is still incipient. According to the available literature, only one species is recorded to this country, Hyrmophlaeba magdalena Bernardi, 1977.

Interannual hydroclimatic variability and the 2009-2011 extreme ENSO phases in Colombia: from Andean glaciers to Caribbean lowlands

Science.gov (United States)

Bedoya-Soto, Juan Mauricio; Poveda, Germán; Trenberth, Kevin E.; Vélez-Upegui, Jorge Julián

2018-03-01

During 2009-2011, Colombia experienced extreme hydroclimatic events associated with the extreme phases of El Niño-Southern Oscillation (ENSO). Here, we study the dynamics of diverse land-atmosphere phenomena involved in such anomalous events at continental, regional, and local scales. Standardized anomalies of precipitation, 2-m temperature, total column water (TCW), volumetric soil water (VSW), temperature at 925 hPa, surface sensible heat (SSH), latent heat (SLH), evaporation (EVP), and liquid water equivalent thickness (LWET) are analyzed to assess atmosphere-land controls and relationships over tropical South America (TropSA) during 1986-2013 (long term) and 2009-2011 (ENSO extreme phases). An assessment of the interannual covariability between precipitation and 2-m temperature is performed using singular value decomposition (SVD) to identify the dominant spatiotemporal modes of hydroclimatic variability over the region's largest river basins (Amazon, Orinoco, Tocantins, Magdalena-Cauca, and Essequibo). ENSO, its evolution in time, and strong and consistent spatial structures emerge as the dominant mode of variability. In situ anomalies during both extreme phases of ENSO 2009-2011 over the Magdalena-Cauca River basins are linked at the continental scale. The ENSO-driven hydroclimatic effects extend from the diurnal cycle to interannual timescales, as reflected in temperature data from tropical glaciers and the rain-snow boundary in the highest peaks of the Central Andes of Colombia to river levels along the Caribbean lowlands of the Magdalena-Cauca River basin.

Aves ocasionales en la Sabana de Bogotá

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Borrero José Ignacio

1946-08-01

Full Text Available La altiplanicie andina conocida con el nombre de Sabana de Bogotá, situada en la llamada "tierra fría" de los Andes Orientales de Colombia, a 2600 metros sobre el nivel del mar y con temperatura media de 13o C., esta bordeada en todo su derredor por montañas cuya altura en ningún sitio se eleva a menos de 2700 metros y esto solamente en el lado occidental, hacia el valle del Magdalena. Sin embargo, es visitada con alguna frecuencia por aves propias de pisos climáticos más cálidos y aun del llamado "Tropical" o "tierra caliente". Por lo que se ha podido averiguar, las aves visitantes observadas hasta ahora proceden de las vertientes occidentales y del valle del Magdalena; ninguna, con la única posible excepción de Elanoides forticatus yetapa, viene de las faldas orientales que descienden hasta los llanos del Meta pues hacia este lado de la Sabana se hallan las montañas más altas, cuya mayor depresión -la de Chipaque- es de 3150 metros en el sitio por donde pasa la carretera de Bogotá a Villavicencio. Parece por lo tanto que este ramal de la cordillera constituye una barrera infranqueable para las aves que habitan en el oriente de Cundinamarca, mientras que el ramal occidental, de menor altura, no lo es para las que habitan en las vertientes que conducen al Magdalena.

Extra Strong Super Light / Magdalena Lewoc

Index Scriptorium Estoniae

Lewoc, Magdalena

2003-01-01

2003. a. Szczecini Rahvusmuuseumis toimunud 5. rahvusvahelisest kunstinäitusest võtsid osa poola, saksa, rootsi, soome, leedu, läti, ukraina ja eesti kunstnikud. Pikemalt Ene-Liis Semperi ja Jaan Toomiku loomingust

Poola Nike 2007 / Hendrik Lindepuu

Index Scriptorium Estoniae

Lindepuu, Hendrik

2007-01-01

Poola kirjanduspreemiast Nike, 2007. aasta laureaadist (Wieslav Mysliwski "Traktaat ubade puhastamisest"), 2007. aasta lugejapreemia laureaadist (Mariusz Szczygiel "Gottland") ja nominentitest (Jerzy Pilch "Minu esimene enesetapp", Magdalena Tull "Defekt", Maria Janion "Ebatavaline slaavilikkus", Tomasz Rozyck "Kolooniad", Marcin Swietlick "Sisemine muusika")

Bagatellid : maailm / Nele-Eva Steinfeld

Index Scriptorium Estoniae

Steinfeld, Nele-Eva

2008-01-01

Akadeemik Jarvis väidab, et osade Bachi teoste autoriks on hoopis helilooja naine Anna Magdalena. Pianist Andras Schiff pälvis Wigmore'i medali. Lahkus tenor Gianni Raimondi. Louvre ja tänapäevane muusikaelu. Uus esiviiul Juliardi keelpillikvartetis

Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.

Science.gov (United States)

Wang, Li Kai; Nair, Pravin A; Shuman, Stewart

2008-08-22

NAD(+)-dependent DNA ligases (LigAs) are ubiquitous in bacteria and essential for growth. LigA enzymes have a modular structure in which a central catalytic core composed of nucleotidyltransferase and oligonucleotide-binding (OB) domains is linked via a tetracysteine zinc finger to distal helix-hairpin-helix (HhH) and BRCT (BRCA1-like C-terminal) domains. The OB and HhH domains contribute prominently to the protein clamp formed by LigA around nicked duplex DNA. Here we conducted a structure-function analysis of the OB and HhH domains of Escherichia coli LigA by alanine scanning and conservative substitutions, entailing 43 mutations at 22 amino acids. We thereby identified essential functional groups in the OB domain that engage the DNA phosphodiester backbone flanking the nick (Arg(333)); penetrate the minor grove and distort the nick (Val(383) and Ile(384)); or stabilize the OB fold (Arg(379)). The essential constituents of the HhH domain include: four glycines (Gly(455), Gly(489), Gly(521), Gly(553)), which bind the phosphate backbone across the minor groove at the outer margins of the LigA-DNA interface; Arg(487), which penetrates the minor groove at the outer margin on the 3 (R)-OH side of the nick; and Arg(446), which promotes protein clamp formation via contacts to the nucleotidyltransferase domain. We find that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation.

Enzyme-linked electrochemical DNA ligation assay using magnetic beads.

Science.gov (United States)

Stejskalová, Eva; Horáková, Petra; Vacek, Jan; Bowater, Richard P; Fojta, Miroslav

2014-07-01

DNA ligases are essential enzymes in all cells and have been proposed as targets for novel antibiotics. Efficient DNA ligase activity assays are thus required for applications in biomedical research. Here we present an enzyme-linked electrochemical assay based on two terminally tagged probes forming a nicked junction upon hybridization with a template DNA. Nicked DNA bearing a 5' biotin tag is immobilized on the surface of streptavidin-coated magnetic beads, and ligated product is detected via a 3' digoxigenin tag recognized by monoclonal antibody-alkaline phosphatase conjugate. Enzymatic conversion of napht-1-yl phosphate to napht-1-ol enables sensitive detection of the voltammetric signal on a pyrolytic graphite electrode. The technique was tested under optimal conditions and various situations limiting or precluding the ligation reaction (such as DNA substrates lacking 5'-phosphate or containing a base mismatch at the nick junction, or application of incompatible cofactor), and utilized for the analysis of the nick-joining activity of a range of recombinant Escherichia coli DNA ligase constructs. The novel technique provides a fast, versatile, specific, and sensitive electrochemical assay of DNA ligase activity.

Processing and characterization of screen printing Ba 0.5 Sr 0.5 Co ...

Indian Academy of Sciences (India)

Author Affiliations. MAGDALENA GROMADA1 DAVIDE GARDINI2 PIETRO GALIZIA2 CARMEN GALASSI1. Institute of Power Engineering Ceramic Department CEREL, Research Institute, 1 Techniczna St., 36-040 Boguchwała, Poland; National Research Council (CNR)—Institute of Science and Technology for Ceramics ...

Läänemeresoome keelte õppetoolis : soome keele olümpiaad Agricola päeval / Maris Amon

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Amon, Maris

2000-01-01

7. aprillil Agricola päeva tähistava soome keele olümpiaadi korraldusega on Tallinna Pedagoogikaülikoolist seotud professor Jaan Õispuu žürii koosseisus, olümpiaadi ülesandeid koostavad õppejõud Maria-Magdalena Jürvetson ja Tarja Tanttu

"Who's There?": Shakespeare and the Dragon of Autism

Science.gov (United States)

Renino, Christopher

2009-01-01

Dan recently turned 13; Nick will soon be 12. Both boys are smart and fun; both are loving, write like angels, like to learn, and want friends; both are autistic and hope to learn to speak. They find it challenging to relate to and function in this world, and they work hard to improve their abilities to do so. Last fall, Nick and Dan became…

ANÁLISIS DE LA EFECTIVIDAD DEL USO DE LA PLATAFORMA VIRTUAL WEBCT EN EL PROCESO DE ENSEÑANZA Y APRENDIZAJE EN LA UNIVERSIDAD DEL MAGDALENA, COLOMBIA

Directory of Open Access Journals (Sweden)

MÓNICA LUZ PÉREZ CERVANTES

2012-01-01

Full Text Available En las carreras de pregrado en Educación de la Universidad del Magdalena, la mayor parte de los docentes adoptan un modelo de enseñanza tradicional, pese a que las TIC ofrecen la posibilidades de interacción docente - estudiantes, no la utilizan de manera asertiva en sus clases, debido a que consideran que sólo resultan útiles en la educación a distancia. En los últimos tres años, un grupo reducido de docentes han comenzado a incorporar, de manera tímida, la plataforma WebCT como escenarios complementarios a la clase presencial, pero no se han realizado investigaciones que recojan las percepciones de los actores, que demuestren la efectividad de la utilización combinada de actividades colaborativas presenciales con las actividades apoyadas en la plataforma virtual. El propósito de la investigación fue analizar la efectividad de la plataforma WebCT en el desarrollo de un curso de formación disciplinar impartido de manera tradicional presencial complementado con enseñanza virtual, apoyada en los aportes de Vygotsky, Ausubel, Pozo, Coll, Marqués, Sangrá, entre otros, realizada de acuerdo con el paradigma cualitativo, con un enfoque descriptivo y con un diseño correspondiente a un Estudio de caso, en el cual se utilizó como técnicas el grupo focal y dos encuestas que permitieron apreciar la opinión de los estudiantes acerca del uso de las TIC y uso de la plataforma WebCT, así como la interacción en las actividades desarrolladas durante el curso en la plataforma virtual. Para ello se contó con la participación de 70 estudiantes de Licenciatura en Informática en el primer semestre académico de 2010. Los resultados indican que en el modelo didáctico híbrido se combinan la responsabilidad, rapidez y calidad de respuesta del docente con la participación fluida del estudiante en la realización de actividades y entregas de trabajos, por los aportes al grupo a través de los foros y del Chat, así como la motivación generada

Contribución al conocimiento de los Machiloidea de Colombia (Archaeognatha: Insecta Contribución al conocimiento de los Machiloidea de Colombia (Archaeognatha: Insecta

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Sturm Helmut

1983-09-01

Full Text Available Se describen cuatro nuevas especies y una subespecie de Meinertellidae recolectadas en Colombia y en un bosque pluvial cerca de Manaos, Brasil. Son las siguientes: Meinertellus adisi, M. bogotensis santaemartae, Neomachilellus aboucbaari, N. amazonicus y N. rangeli. Se discute la posición sistemática de los ejemplares recolectados cerca de Bogotá, en el valle del río Magdalena y en la Cordillera Oriental cerca de Neiva. Se da una clave para los géneros suramericanos de la familia.Four new species and one new subspecies of Meinertellidae occurring respectively in Colombia and in the rain forest near Manaus, Brazil, are described:  Meinertellus adisi, M. bogotensis santaemartae , Neomachilellus abouchaari, N. amazonicus and N. rangeli. The systematical position of specimens collected near Bogota, in the Magdalena Valley and in the Eastern Andes near Neiva is discussed. A key for the South American genera of the family is given.

Distribution of tortoises and freshwater turtles of the Colombian Caribbean

International Nuclear Information System (INIS)

Montes Correa, Andres Camilo; Saboya Acosta, Liliana Patricia; Paez, Vivian; Vega, Karen; Renjifo, Juan Manuel

2014-01-01

This research reviews the Colombian Caribbean distribution of the species Kinosternon scorpioides, Trachemys callirostris, Mesoclemmys dahli and Chelonoidis carbonaria, and to present new records for the region. The species K. scorpioides is reported for the first time in the Manzanares River drainage, Santa Marta, Department of Magdalena. Trachemys callirostris was recorded in the Canas River, Department of La Guajira, being the first record for this species in a small river on the north side of the Sierra Nevada de Santa Marta. Chelonoidis carbonaria was recorded in a wetland in Santa Marta. We recorded a female M. dahli in the village of Monterrubio, municipality of Sabanas de San Angel, Department of Magdalena. Three of the four species included in this account are listed in some category of threat. The lack of knowledge of the biology and distribution of these species could be considered a threat to them because ignorance precludes the establishment of their true conservation status and hinders the development of management plans required for their protection.

ISOLDE’s rarest isotopes

CERN Multimedia

Katarina Anthony

2013-01-01

The ISOLDE physics section (PH-SME-IS) has recently observed an unusual transformation… one that may well be a sign of things to come for CERN.   The ISOLDE PH team, from left to right: Jennifer Weterings (user support), Susanne Kreim (research fellow), Marek Pfützner* (scientific associate), Maria Garcia Borge (team leader), Elisa Rapisarda (research fellow) , Magdalena Kowalska (physics coordinator), Jan Kurcewicz* (applied fellow), Monika Stachura (future applied fellow). Not in the photo: Kara Lynch (PhD student). Although more and more women are going into the physical sciences, it’s no secret that it remains a male-dominated field. But over the past six months, the ISOLDE PH section has become an exception to this rule: with female physicists outnumbering their male colleagues 3 to 1. “We’re probably one of the few teams at CERN where the personnel is mostly female,” says Magdalena Kowalska, the ISOLDE Physics Coordinator. &am...

Mechanical Design of NESSI: New Mexico Tech Extrasolar Spectroscopic Survey Instrument

Science.gov (United States)

Santoro, Fernando G.; Olivares, Andres M.; Salcido, Christopher D.; Jimenez, Stephen R.; Jurgenson, Colby A.; Hrynevych, Michael A.; Creech-Eakman, Michelle J.; Boston, Penny J.; Schmidt, Luke M.; Bloemhard, Heather;

Lazareto de Agua de Dios, Frente a los Valores Sociales Invertidos // Lazareto de Agua de Dios, in Front of Invested Social Securities // Lazareto Água de Dios, Contra a Valores Sociais Investidos

Directory of Open Access Journals (Sweden)

Darío Benavides Pava

2016-12-01

Full Text Available El presente artículo forma parte del proyecto de investigación institucional denominado Historiografía del Empresariado de Cundinamarca 1900-2010; Capítulo: Provincia del Alto Magdalena. Con este estudio se pretende hacer un merecido reconocimiento al empresariado de la provincia que, con su esfuerzo y tenacidad lograron hacer del Alto Magdalena una región próspera, foco de inversión nacional y extranjera. De igual manera, interesa a la academia analizar los modelos y técnicas administrativas empleados por los emprendedores locales en el empeño por sacar adelante sus proyectos. Este estudio lleva a cabo una secuencia documental de carácter histórico que permite conocer el modelo administrativo implementado en el Lazareto de Agua de Dios y su particular sistema económico, dando ejemplo de funcionalidad con respeto por la dignidad humana. Fue de esta manera como la sociedad resolvió “el problema”. 

The preparation of radioactivity labelled DNA

International Nuclear Information System (INIS)

Ballance, P.; Morgan, J.; McGregor, G.; Durkacz, B.

1984-01-01

The nick translation reaction, which uses the endonuclease enzyme to incorporate pieces of DNA into the genetic material of other organisms, is being used more and more in Molecular Biology research for the preparation of pure DNA labelled with 32 P. However results are presented which show that high radiation doses are received by the hands of nick translation workers. A Scheme of Work to reduce these doses is described. (author)

Use of Performance Measures to Evaluate, Document Competence and Deterioration of ASSET Surgical Skills

Science.gov (United States)

2016-05-01

meeting with Advisory Board Member, Prof Nick Sevdalis (London UK) in Seattle at the ASE meeting (April 21-25 2015) to address how to validate our...cognitive task analysis and human factors, and Prof. Nick Sevdalis , an experimental psychologist and expert in patient safety in hospital...Individual Procedure Score (IPS) performance metric and to update Prof Sevdalis on project progress.  Presented a poster and 2 podium presentation at

Landscapes of The Mind: The Setting as Psyche in Nathaniel Hawthorne's The Scarlet Letter And F. Scott Fitzgerald's The Great Gatsby Landscapes of The Mind: The Setting as Psyche in Nathaniel Hawthorne's The Scarlet Letter And F. Scott Fitzgerald's The Great Gatsby

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Cruce Stark

2009-09-01

Full Text Available In the closing moment of F. Scott Fitzgerald's The Great Gateby, Nick returns for one last look at what once was Gateby's house. But instead of physical buildings, he has a vision of an earlier time, a vision of the "old island" that "flowered once for Dutch sailor's eyes -- a fresh green breast of the new world." "For a transitory enchanted moment," Nick thinks, "man must have held his breath in the presence of this continent, compelled into an aesthetic contemplation he neither understood nor desired, face to face for the last time in history with something commensurate to his capacity for wonder (182." In the closing moment of F. Scott Fitzgerald's The Great Gateby, Nick returns for one last look at what once was Gateby's house. But instead of physical buildings, he has a vision of an earlier time, a vision of the "old island" that "flowered once for Dutch sailor's eyes -- a fresh green breast of the new world." "For a transitory enchanted moment," Nick thinks, "man must have held his breath in the presence of this continent, compelled into an aesthetic contemplation he neither understood nor desired, face to face for the last time in history with something commensurate to his capacity for wonder (182."

Geology of a Stable Intraplate Region: The Cape Verde/Canary Basin,

Science.gov (United States)

1982-03-01

other limited area. The National Geophysical and areas, sounding tracks are sufficiently Solar -Terrestrial Data Center (NGSDC) dense to permit...Scientists. New York. McGraw-Hill, 471 p. Ball. M.M. (1969). Discussion Diapers of Magdalena Delta. Bull. Am. Assn. Petrol. Burke, K. and J.T. Wilson (1972

State of the soft bottoms of the continental shelf

International Nuclear Information System (INIS)

Guzman Alvis, Angela I; Solano, Oscar David

2002-01-01

The presented information, it is based on studies carried out on the continental shelf of the Colombian Caribbean, mainly in the Gulf of Morrosquillo and the Magdalena and Guajira departments in the last ten years. A diagnostic is done of the soft bottoms of the Colombian continental shelf

A view from the top: US enrichment Corp.'s William H. Timbers, Jr

International Nuclear Information System (INIS)

Giltenan, E.

1993-01-01

Nick Timbers took over as the first Transition Manager of the US Enrichment Corporation upon its founding last July 1st. Although USEC is not involved in negotiating the HEU deal, the fledgling company will be in charge of actually buying and selling the resulting LEU. Whenever the deal is finally signed. After the politics and haggling are over, it will be up to Nick Timbers to make the deal work on the global uranium market. The view from USEC is resolute. No matter what shape the final HEU deal takes, Nick Timbers promises that USEC will remain a competitive supplier of enrichment services. Timbers pledges that any extra costs associated with the HEU deal will not be passed on to customers. He took time out from his recent busy schedule to share his thoughts on HEU and its aftermath

EFECTO DE TILAPIA Oreochromis niloticus SOBRE LA PRODUCCIÓN PESQUERA DEL EMBALSE EL GUÁJARO ATLÁNTICO - COLOMBIA

Directory of Open Access Journals (Sweden)

Pedro Caraballo G, M.Sc.

2009-12-01

Full Text Available Objetivo. Evaluar el efecto de tilapia Oreochromis niloticus sobre la producción pesquera en el embalse de El Guájaro, departamento del Atlántico, Colombia. Materiales y métodos. El embalse tiene un área aproximada de 14.000 ha y allí pescan diariamente 2.500 pescadores provenientes de ocho municipios que rodean el ecosistema. Por medio de evaluaciones mensuales del desembarco durante 48 horas en todos los puertos, fue evaluada la composición y abundancia de las capturas en 1988 y 2002. Los resultados de la evaluación hecha en 2002 fueron comparados con los obtenidos en 1988. Resultados. 38 especies de peces, perteneciendo a 14 familias fueron identificadas. Sólo las dos especies que dominan las capturas, presentaron una variación en su participación global. La producción durante 2002 fue de 431 ton/mes, superior a las 84 ton/mes evaluadas en el año 1988. Durante 2002, las capturas fueron dominadas por tilapia Oreochromis niloticus (53% y arenca Triportheus magdalenae (36% lo que representa una variación en la composición de las capturas que, durante 1988 fueron respectivamente de 13% y 73%. Esta variación no afectó la proporción de herbívoros y carnívoros que se mantuvo en 90-10%. Conclusiones. La variación en la composición y abundancia de la producción total implica el desplazamiento de una especie nativa (Triportheus magdalenae por una exótica (Oreochromis niloticus, hecho que viene siendo observado en toda la cuenca del Magdalena en esta década.

Crossing the street

DEFF Research Database (Denmark)

Shepherd, Nick

2001-01-01

Nick Shepherd teaches a class on 'race and racism' at the University of Cape Town. Here he shares the journey he undertakes with his students to find a "civil space'' to discuss sensitive and complex issues.......Nick Shepherd teaches a class on 'race and racism' at the University of Cape Town. Here he shares the journey he undertakes with his students to find a "civil space'' to discuss sensitive and complex issues....

Santa Magdalena de les Tragines Altarpiece. Epilogue

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Riu de Martín, M. Carme

2007-06-01

Full Text Available

This is a work review of the article published in “Anuario de Estudios Medievales” 36/1 (2006, pp. 355-284. After the restoration of the pieces one has obtained new data about their iconography and their image’s symbolism.

Invasive American bullfrogs and African clawed frogs in South America

DEFF Research Database (Denmark)

Barbosa, Fabiana G.; Both, Camila; Bastos, Miguel

2017-01-01

.5% of the protected areas within the Atlantic Forest may be most at risk of invasion by L. catesbeianus and X. laevis under current climate conditions, followed by areas in the Cerrado (51.7), Tropical Andes (37.6%), Tumbes-Choco-Magdalena (22.5%), and Chilean Winter Rainfall and Valdivian Forests (20...

New departmental records for Digitonthophagus gazella (Coleoptera: scarabaeidae) in Colombia

International Nuclear Information System (INIS)

Noriega, Jorge Ari; Moreno, Juliana; Otavo, Samuel; Castano, Eduardo

2012-01-01

The introduced dung beetle Digitonthophagus Gazella was reported for the Caribbean Region of Colombia in the 90's. During a survey carried out along the Magdalena River, new records of the presence of this species were found in the departments of Boyaca, Caldas, Cundinamarca, Santander and Tolima for the first time.

Cerambycidae (Coleoptera da Colômbia. V. Lamiinae com unhas tarsais divergentes Cerambycidae (Coleoptera of Colombia. V. Lamiinae with divergent tarsal claws

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Maria Helena M. Galileo

2003-06-01

Full Text Available New species described from Colombia: Amphicnaeia interrupta sp. nov., from Cauca; Adetus tayronus sp. nov., from Magdalena; A. aberrans sp. nov., from Santander; Parmenonta albosticta sp. nov., from Cundinamarca; Jamesia bella sp. nov., from Cauca. New records for nine species of Colombian Lamiinae (Apomecynini, Agapanthiini, Onciderini are given.

Greens protest at U. S. Embassy / Nick Coleman

Index Scriptorium Estoniae

Coleman, Nick

2000-01-01

Protestijad kogunesid USA Riia saatkonna hoone ette. Väljendati oma rahulolematust USA tahtmatuse suhtes vähendada süsinikdioksüüdi paiskamist õhkkonda, mis ilmnes eelmisel kuul peetud kliimamuutuse konverentsil Haagis

Review Article: The Vanished Ghosts in Two Hungarian Family Memoirs. Farkas, Charles. 2013. Vanished by the Danube: Peace, War, Revolution, and Flight to the West (Introduction by Margaret McMullan. Albany: Excelsior Editions, State University of New York Press. 472 pp. Illus; and Barlay, Nick. 2013. Scattered Ghosts: One Family's Survival through War, Holocaust and Revolution. London and New York: I. B. Tauris. 240 pp. Illus.

Directory of Open Access Journals (Sweden)

Ruth G. Biro

2015-01-01

Full Text Available Recent personal documentary works about major historical events of the twentieth century, e.g., World War II, the Holocaust and the 1956 Hungarian Revolution, offer their readers a rich and multifaceted narrative, or a history that is also "his story," "her story" and that of entire families, cohorts and communities. Often, these works are accompanied by visual artifacts such as photographs, family tress, maps etc., or supported by concise historical surveys. Thus these memoirs complete the work of historians with the lived experiences of the few that represent many. Such is the case with two 2013 books by Charles Farkas and Nick Barlay depicting their mid-twentieth century Hungarian families, one Christian and one Jewish, through two World Wars and the anti-communist uprising, culminating in their escape to the West and in the two authors looking back upon the Hungarian past of their families.

Fácies Orgânica da Formação La Luna, Bacia do Valle Superior del Magdalena, Colômbia:Implicações para a Geração de Petróleo e Caracterização Paleoambiental.

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Juliana Andrade Iemini

2007-08-01

Full Text Available Upper Cretaceous pelitic rocks generate most of the oil reserves in Colombia. In Del Magdalena Upper Valley (MUV, the existenceof good quality outcrops and thermal evolution levels that vary from immaturity to the end of the oil generation “window” makes it one of themost appropriate regions for the study of primary migration and generation of oil in Upper Cretaceous. The main objectives of this work wereto study the organic facies of La Luna Formation in an outcrop section called Riacho Bambuca (Bambuca Creek, determine the oil generationpotential and characterize the paleoenvironment. For this research, 24 samples were used in palynofacies analysis (characterization ofthe kerogen components assembly and in organic geochemistry analysis (Total Organic Carbon – TOC analysis and Rock-Eval pyrolysis,provided by Colombia Oil Institute – ECOPETROL. The palynofacies analysis included the use of microscopy techniques (transmitted whitelight and incident blue light. Furthermore, a Spore Coloration Index (SCI analysis was made in order to determine the thermal maturationstage. The studied material was composed mainly of an amorphous organic substance, with its fluorescent coloration varying from yellow tobright orange. The palynomorph group is represented by spore morph and dinoflagellate cysts. The ligneous material are present in very lowpercentages; they are mainly opaque phytoclasts. The results of geochemical analysis showed TOC contents ranging from medium to high,excellent potential for the generation of hydrocarbons (PG > 4 mg HC/g of rock, hydrogen index ranging from 329 to 589 mg HC/g, andlow values of oxygen index, characterizing type II kerogen. The low degree of thermal maturation was determined by Spore Coloration Indexvalues ranging from 2,5 to 3,0, Tmax values below 440°C and Hydrocarbon Production Index inferior to 0,2. According to the organic faciesdata, La Luna Formation deposited in a marine environment with facies from

Lack of evidence that the XqYq pairing tips at meiosis in the mouse show hypersensitivity to DNAse I.

Science.gov (United States)

Separovic, E R; Chandley, A C

1987-01-01

In situ nick translation procedures have been applied to meiotic metaphase I divisions of the normal and XY, Sxr mouse. Unlike in man, where the pairing tips of the XY bivalent show a special sensitivity to DNAse I nicking, no such sensitivity can be detected for either of these types of mouse. Hypersensitivity in the D-band equivalent region of the X chromosome does, however, exist, this site being early replicating in somatic cells and housing the X inactivation centre (Xce).

Substrate specificity of Micrococcus luteus uv endonuclease and its overlap with DNA photolyase activity

International Nuclear Information System (INIS)

Patrick, M.H.

1975-01-01

The action of an endonuclease from Micrococcus luteus that operates on uv damage in DNA overlaps with that of DNA photolyase from yeast: homo- and heterocyclobutane dipyrimidines in DNA are substrates for both enzymes, but pyrimidine adducts or the spore photoproduct in DNA are not. As expected from this overlap, the action of the two enzymes is mutually interfering: single-strand nicks introduced by the endonuclease effectively preclude photoreactivation; conversely, formation of a photolyase-cyclobutane dipyrimidine complex can prevent nicking by the endonuclease

Hatten af for Cohens skønheder og skyggesider

DEFF Research Database (Denmark)

Mortensen, Lise Majgaard

2011-01-01

Der er højt til loftet i Aarhus Teaters nye teaterkoncert, som behandler selveste Leonard Cohens lyriske univers. Det er svært at lade være med at sammenligne forestillingen her med Nick Cave Teaterkoncerten, der gik sin sejrsgang i 2007 og 2008. Men det skal man ikke. Instruktøren er den samme....... Men hvor ’Nick Cave’ var rå, grim, provokerende og uanstændig, er ’Leonard Cohen’ en skrøbelig gentleman med en skyggeside....

Mercury in the gold mining district of San Martin de Loba, South of Bolivar (Colombia).

Science.gov (United States)

Olivero-Verbel, Jesus; Caballero-Gallardo, Karina; Turizo-Tapia, Alexi

2015-04-01

Gold mining is responsible for most Hg pollution in developing countries. The aims of this study were to assess the levels of total Hg (T-Hg) in human hair, fish, water, macrophyte, and sediment samples in the gold mining district of San Martin de Loba, Colombia, as well as to determine fish consumption-based risks for T-Hg ingestion. T-Hg levels were measured by electrothermal atomization and atomic absorption spectroscopy. The overall mean T-Hg level in hair for humans in the mining district of San Martin de Loba was 2.12 μg/g, whereas for the reference site, Chimichagua, Cesar, it was 0.58 μg/g. Mean T-Hg levels were not different when considered within localities belonging to the mining district but differed when the comparison included Chimichagua. T-Hg levels in examined locations were weakly but significantly associated with age and height, as well as with fish consumption, except in San Martin de Loba. High T-Hg concentrations in fish were detected in Pseudoplatystoma magdaleniatum, Caquetaia kraussii, Ageneiosus pardalis, Cyrtocharax magdalenae, and Triportheus magdalenae, whereas the lowest appeared in Prochilodus magdalenae and Hemiancistrus wilsoni. In terms of Hg exposure due to fish consumption, only these last two species offer some guarantee of low risk for Hg-related health problems. Water, floating macrophytes, and sediments from effluents near mining sites also had high Hg values. In mines of San Martin de Loba and Hatillo de Loba, for instance, the geoaccumulation index (I(geo)) for sediments reached values greater than 6, indicating extreme pollution. In short, these data support the presence of a high Hg-polluted environment in this mining district, with direct risk for deleterious effects on the health of the mining communities.

Taxonomic revision and phylogenetic relationships of Dasyloricaria Isbrücker & Nijssen, 1979 (Siluriformes: Loricariidae, with description of a new species

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Alejandro Londoño-Burbano

Full Text Available ABSTRACT A taxonomic revision and phylogenetic analysis were completed for Dasyloricaria . The genus includes three valid species: D . filamentosa and D . latiura previously included in the genus, and a new species described herein. Dasyloricaria have a restricted trans-Andean distribution, with D . filamentosa occurring at the lower and middle Magdalena, lower Cauca, and Sinu in Colombia, and lago Maracaibo basin in Colombia and Venezuela; D . latiura in the Atrato and the Tuyra basins in Colombia and Panama, respectively; and the new species in the upper and middle Magdalena basin in Colombia. New synonyms for D . filamentosa and D . latiura are proposed, and a lectotype is designated for the latter. Dasyloricaria is herein recognized as monophyletic, with D . filamentosa as the sister group of D . latiura , and the new speciesas sister to that clade. Spatuloricaria is hypothesized to be the sister group of Dasyloricaria based on synapomorphies of the neurocranium, branchial arches and external morphology features. The subtribe Rineloricariina was partially corroborated through the phylogenetic analysis. An identification key for the species of Dasyloricaria is provided.

NEW DEPARTMENTAL RECORDS OF DIGITONTHOPHAGUS GAZELLA (COLEOPTERA: SCARABAEIDAE IN COLOMBIA

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Jorge Ari Noriega

2012-01-01

Full Text Available The introduced dung beetle Digitonthophagus gazella was reported for the Caribbean Region of Colombia in the 90’s. During a survey carried out along the Magdalena River new records of the presence of this species were found in the departments of Boyacá, Caldas, Cundinamarca, Santander and Tolima for the first time.

Rembrandt Kadrioru lossis / Jüri Hain

Index Scriptorium Estoniae

Hain, Jüri, 1941-

2000-01-01

Kadrioru lossi kuppelsaali tundmatu meistri laemaali maalimise ajalugu. Maali aluseks on Rembrandti maal "Diana suplus". Laemaal on maalitud Magdalena de Passe (1600-1638) vasegravüüri põhjal, mis on tehtud Rembrandti maalist. Rembrandt on lähtunud Antonio Tempesta (1555-1630) kahest gravüürist, viimane on lähtunud Otto van Veeni (1556-1629) maalist

Pilk kahe keelemehe pikale kirjavahetusele / Paul Alvre

Index Scriptorium Estoniae

Alvre, Paul

2004-01-01

Arvustus: Baudouin de Courtenay, Jan. Briefwechsel zwischen Jan Baudouin de Courtenay und Jooseppi J. Mikkola aus den Jahren 1898-1926 = Jan Baudouin de Courtenay i Jooseppi J. Mikkola korespondencja z lat 1898-1926 / herausgegeben von Raimo Pullat in Zusammenarbeit mit Magdalena Smoczynska. Krak̤w : Polska Akademia Umiejetnosci, 2004. (Studia i materialy do dziej̤w Polskiej Akademii Umiejetnosci ; 4)

Environmental Education and the Bogotá River: An Intervention to Be Carried out in Cundinamarca (Colombia)

Science.gov (United States)

Jefferson, Galeano Martínez; Ciro, Parra Moreno; Méndez Sánchez, Maria Andrea

2017-01-01

The Bogotá River is one of the most contaminated bodies of water in Colombia and in the world. It originates in Guacheneque Páramo (Villapinzón, Cundinamarca) in the centre of the country and runs 336 kilometres before joining the Magdalena River. Along its course, the river receives the sewage of approximately 20.9% of Columbia's population. The…

AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination.

Science.gov (United States)

Kobayashi, Maki; Aida, Masatoshi; Nagaoka, Hitoshi; Begum, Nasim A; Kitawaki, Yoko; Nakata, Mikiyo; Stanlie, Andre; Doi, Tomomitsu; Kato, Lucia; Okazaki, Il-mi; Shinkura, Reiko; Muramatsu, Masamichi; Kinoshita, Kazuo; Honjo, Tasuku

2009-12-29

To initiate class switch recombination (CSR) activation-induced cytidine deaminase (AID) induces staggered nick cleavage in the S region, which lies 5' to each Ig constant region gene and is rich in palindromic sequences. Topoisomerase 1 (Top1) controls the supercoiling of DNA by nicking, rotating, and religating one strand of DNA. Curiously, Top1 reduction or AID overexpression causes the genomic instability. Here, we report that the inactivation of Top1 by its specific inhibitor camptothecin drastically blocked both the S region cleavage and CSR, indicating that Top1 is responsible for the S region cleavage in CSR. Surprisingly, AID expression suppressed Top1 mRNA translation and reduced its protein level. In addition, the decrease in the Top1 protein by RNA-mediated knockdown augmented the AID-dependent S region cleavage, as well as CSR. Furthermore, Top1 reduction altered DNA structure of the Smu region. Taken together, AID-induced Top1 reduction alters S region DNA structure probably to non-B form, on which Top1 can introduce nicks but cannot religate, resulting in S region cleavage.

Gumucio y su cámara, Magdalena abajo

Directory of Open Access Journals (Sweden)

Amparo Cadavid Bringe

2011-12-01

Full Text Available La autora narra una experiencia de trabajo en una comunidad colombiana con comunicadores de base y parte del aporte de Gumucio Dagron a la bibliografía de comunicación para el desarrollo y su mayor aporte a la realización de los proyectos basados en sus textos analíticos. Teoría y práctica se palpan en este texto.

Kestab Euroopa keelte aasta / Maria-Magdalena Jürvetson

Index Scriptorium Estoniae

Jürvetson, Maria-Magdalena, 1952-

2001-01-01

TPÜ põhjamaade filoloogia osaonnas toimus 21.-23. sept. 2001.a. "Nordic Grant Scheme for Network Cooperation" projekti raames rahvusvaheline seminar teemal "From multilingual to multicultural Europe in the year of languages 2001"

The Portraiture of Nick: Scene Two, the Middle Years

Science.gov (United States)

Bicehouse, Vaughn L.

2012-01-01

In 2007, the Autism Society of America (ASA) estimated that 1.5 million Americans and their families were affected by autism. As the current Autistic Spectrum Disorder (ASD) prevalence rates continue to rise (10%-17% each year) so does the awareness that ASD is no respecter of persons. ASD touches children of every racial, ethnic, and…

Im Wind / Mari Vallisoo ; tõlk. Gisbert Jänicke

Index Scriptorium Estoniae

Vallisoo, Mari, 1950-2013

2003-01-01

Sisu: Im Wind = Tuulega ; Zwei Frauen = Kaks naist ; In Gedanken an Aleksander Levin = aleksander Levinile mõteldes ; Jetzt im Dunkel = Nüüd pimedas ; Fragen = Küsimused ; Der Zwillingsbruder = Kaksikvend

Ten years of transition and... / Rika Ishii, Nick Sten

Index Scriptorium Estoniae

Ishii, Rika

1999-01-01

Ülevaade majandusreformidest Baltimaades ja Baltimaade majandussuhetest Euroopa Liidu ning Venemaaga. Tabel: SKP, inflatsioon, väliskaubandus ja välisinvesteeringud Eestis, Lätis, Leedus ja Kesk- ja Ida-Euroopa riikides aastatel 1991-1998

Functional properties and structural requirements of the plasmid pMV158-encoded MobM relaxase domain.

Science.gov (United States)

Fernández-López, Cris; Pluta, Radoslaw; Pérez-Luque, Rosa; Rodríguez-González, Lorena; Espinosa, Manuel; Coll, Miquel; Lorenzo-Díaz, Fabián; Boer, D Roeland

2013-07-01

A crucial element in the horizontal transfer of mobilizable and conjugative plasmids is the relaxase, a single-stranded endonuclease that nicks the origin of transfer (oriT) of the plasmid DNA. The relaxase of the pMV158 mobilizable plasmid is MobM (494 residues). In solution, MobM forms a dimer through its C-terminal domain, which is proposed to anchor the protein to the cell membrane and to participate in type 4 secretion system (T4SS) protein-protein interactions. In order to gain a deeper insight into the structural MobM requirements for efficient DNA catalysis, we studied two endonuclease domain variants that include the first 199 or 243 amino acid residues (MobMN199 and MobMN243, respectively). Our results confirmed that the two proteins behaved as monomers in solution. Interestingly, MobMN243 relaxed supercoiled DNA and cleaved single-stranded oligonucleotides harboring oriTpMV158, whereas MobMN199 was active only on supercoiled DNA. Protein stability studies using gel electrophoresis and mass spectrometry showed increased susceptibility to degradation at the domain boundary between the N- and C-terminal domains, suggesting that the domains change their relative orientation upon DNA binding. Overall, these results demonstrate that MobMN243 is capable of nicking the DNA substrate independently of its topology and that the amino acids 200 to 243 modulate substrate specificity but not the nicking activity per se. These findings suggest that these amino acids are involved in positioning the DNA for the nuclease reaction rather than in the nicking mechanism itself.

Nutrient fluxes and net metabolism in a coastal lagoon SW peninsula of Baja California, Mexico

Directory of Open Access Journals (Sweden)

Cervantes Duarte, R.

2016-09-01

Full Text Available Fluxes of nutrients and net metabolism were estimated in coastal lagoon Magdalena Bay using LOICZ biogeochemical model. In situ data were obtained from 14 sites in the lagoon and also from a fixed site in the adjacent ocean area. Intense upwelling (February to July and faint upwelling (August to January were analyzed from monthly time series. The Temperature, nitrite + nitrate, ammonium and phosphate within the lagoon showed significant differences (p<0.05 between the two periods. Salinity (p=0.408 was more homogeneous (no significantly different due to mixing processes. During the intense upwelling period, nutrients increased in and out of the lagoon due to the influence of Transitional Water and Subartic Water transported by the California Current. However, during the faint upwelling, from August to January, the Transition Water and Subtropical Surface Water were predominant. Magdalena Bay showed denitrification processes of throughout the year as it occurred in other semi-arid coastal lagoons. It also showed a net autotrophic metabolism during intense upwelling and heterotrophic metabolism during faint upwelling. Understanding nutrient flows and net metabolism through simple biogeochemical models can provide tools for better management of the coastal zone.

[Frequency and variables associated with perceived devaluation-discrimination in victims of the armed conflict in Colombia].

Science.gov (United States)

Campo-Arias, Adalberto; Ospino, Anyelly C; Sanabria, Adriana R; Guerra, Valeria M; Caamaño, Beatriz H; Herazo, Edwin

2017-11-21

There is no information on frequency of perceived devaluation-discrimination in victims of the armed conflict in Colombia. The aim of this study was thus to determine the frequency of perceived devaluation-discrimination and associated variables among victims of the armed conflict in municipalities in the Department of Magdalena, Colombia. A cross-sectional study was conducted among victims enrolled in the Program for Psychosocial Care and Comprehensive Healthcare for Victims. Depressive symptoms were quantified with four dichotomous items (three or more were classified as high level of depressive symptoms), and perceived devaluation-discrimination was quantified with six dichotomous items (two or more were classified as high perceived devaluation-discrimination). A total of 943 adults participated (M = 47.9; SD = 14.2); 67.4%, women; 109 (11.6%) reported high level of depressive symptoms and 217 (23%) showed high perceived devaluation-discrimination. High perceived devaluation-discrimination was associated with high level of depressive symptoms (OR = 6.47; 95%CI: 4.23-9.88). In conclusion, one-fourth of the victims of the armed conflict in Magdalena reported high perceived devaluation-discrimination, which was significantly associated with high level of depressive symptoms.

Flood protection as a key-component of the environmental restoration of Canal del Dique, Colombia

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Sokolewicz Marius

2016-01-01

Full Text Available Canal del Dique is a man-made distributary of Rio Magdalena. After its widening in 1980’s environmental degradation caused by abundant sediment load and changes to hydrology took a catastrophic form. In 2010, the Canal’s dike breached and 35,000 ha of land were flooded. In 2013 a huge effort to restore the environment in the Canal del Dique system and to flood-proof the villages was started. An integrated approach was adopted to provide an optimal solution for flood control, environment, fresh water supply and navigation. In order to prepare restoration plans, an extensive hydrologic and hydraulic assessment was carried out. 1D, 2D and 3D numerical models were developed to answer different questions, to evaluate different alternatives and to enable selection of optimal solutions. To assess the flood risk, a hindcast of 2010 flood was carried out. A solution was designed in which the inlet of water from the Magdalena River is regulated by a control structure, managed by an automated system based on SCADA and Delft-FEWS flood forecasting software and advanced statistics.

Comparación de los métodos de inmunoensayo enzimático automatizado (VIDAS y PCR para la detección de Salmonella spp. en expendios de la ciudad de Santa Marta (Colombia

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Libardo Acosta

2013-01-01

Full Text Available Objetivo: comparar los métodos de inmunoensayo enzimático automatizado VIDAS, (Vi- tek Immunodiagnostic Assay System, y el análisis molecular por Reacción en Cadena de la Polimerasa (PCR para detectar Salmonella spp en expendios de la ciudad de Santa Marta (Colombia. Materiales y métodos: Previamente se reportó la estandarización de la técnica de PCR para la detección de Salmonella spp. en un lapso de 12 horas. En colaboración con el Labo- ratorio de Salud Pública del departamento del Magdalena se procedió a tomar un total de 65 muestras de alimentos para análisis de vigilancia rutinaria, discriminados así: carne de res: 14 (21.5 %, embutidos: 18 (27.7 %, pollo: 7(10.8 %, pescado: 3(4.6 %, harinas: 13 (20 %, lácteos: 5 (7.7 % salsas: 4(6.2 % y ensalada: 1(1.5 %, en la ciudad de Santa Marta entre septiembre y noviembre de 2010. Las 65 muestras fueron sometidas a análisis microbiológico para la determinación de Salmonella spp. en el Laboratorio de Salud Pú- blica del departamento del Magdalena siguiendo el protocolo del VIDAS. Una alícuota de 1 mL del preenriquecimiento no selectivo fue enviada refrigerada al laboratorio de biología molecular de la Universidad Cooperativa de Colombia para ser analizada por PCR. Resultados: Los resultados muestran que los alimentos analizados en el Laboratorio de Salud Pública del departamento del Magdalena con VIDAS presentaron Salmonella spp. únicamente en cárnicos: 5/65 (7.7 %, mientras que esas mismas muestras analizadas por PCR mostraban la presencia de Salmonella spp. en 36/65 (55.4 %. Conclusiones: Los resultados indican que la PCR puede ser aplicada para realizar vigilan- cia epidemiológica y obtener resultados cualitativos confiables en menor tiempo.

Compuestos organoclorados residuales en dos especies ícticas de la Ciénaga Grande del Bajo Sinú, Córdoba, Colombia

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Edineldo Lans C

2011-04-01

Full Text Available Objetivo. Determinación de niveles de pesticidas organoclorados (POCs en especies ícticas, procedentes de la Ciénaga Grande del Bajo Sinú, Materiales y métodos. Se usó un cromatógrafo de gases (Perkin Elmer Autosystem XL con detector captura de electrones y columna capilar 5% de difenil y 95% de dimetilpolisiloxano, de 30 m, 0.25 mm de diámetro interno y 0.25 μm de espesor de película. Las especies estudiadas fueron: bocachico (Prochilodus magdalenae y moncholo (Hoplias malabaricus, durante las estaciones seca y húmeda. Resultados. El porcentaje de recuperación de los pesticidas determinados se registró entre 80 y 84%, mientras que los límites de detección y cuanficación fueron 0.02 a 0.18 ng/g y 0.2 a 1.8 ng/g respectivamente. La desviación estándar relativa permaneció por debajo del 7% en todos los casos. De los pesticidas organoclorados investigados, en las dos especies se detectaron el a-clordano, endrín y aldrín. Se observó que a medida que aumenta el peso y el tamaño del pez, aumenta la concentración de los plaguicidas en una de las especies. Los niveles de concentración de los POCs estuvieron en el intervalo de 0.35-3.75 y 0.48-5.49 ng/g para el moncholo (Hoplias malabaricus and bocachico (Prochilodus magdalenae respectivamente. Conclusiones. La frecuencia de aparición de POCs y los niveles detectados en las especies podrían ser consecuencia de actividades antropogénicas realizadas en los alrededores de la ciénaga. La correlación entre las medidas biométricas y la concentración depende de la especie íctica. Las concentraciones encontradas en las especies estudiadas, Hoplias malabaricus y Prochilodus magdalenae no representan toxicidad letal inmediata.

El Carreto o Cumulá - Aspidosperma Dugandii Standl El Carreto o Cumulá - Aspidosperma Dugandii Standl

Directory of Open Access Journals (Sweden)

Dugand Armando

1944-03-01

Full Text Available Nombres vulgares: Carreto (Atlántico, Bolívar, Magdalena; Cumulá, Cumulá (Cundinamarca, ToIima. Según el Dr. Emilio Robledo (Lecciones de Bot. ed. 3, 2: 544. 1939 el nombre Carreto también es empleado en Puerto Berrío (Antioquia. El mismo autor (loc. cit. da el nombre Comulá para una especie indeterminada de Viburnum en Mariquita (Tolima y J. M. Duque, refiriendose a la misma planta y localidad (en Bot. Gen. Colomb. 340, 356. 1943 atribuye este nombre vulgar al Aspidosperma ellipticum Rusby.  Sin embargo, las muestras de madera de Cumulá o Comulá que yo he examinado, procedentes de la región de Mariquita -una de las cuales me fue recientemente enviada por el distinguido ictiólogo Sr. Cecil Miles- pertenecen sin duda alguna al A. Dugandii StandI. Por otra parte, Santiago Cortés (FI. Colomb. 206. 1898; ed, 2: 239. 1912 cita el Cumulá "de Anapoima y otros lugares del (rio Magdalena" diciendo que pertenece a las Leguminosas, pero la brevísima descripción que este autor hace de la madera "naranjada y notable por densidad, dureza y resistencia a la humedad", me induce a creer que se trata del mismo Cumula coleccionado recientemente en Tocaima, ya que esta población esta situada a pocos kilómetros de Anapoima. Nombres vulgares: Carreto (Atlántico, Bolívar, Magdalena; Cumulá, Cumulá (Cundinamarca, ToIima. Según el Dr. Emilio Robledo (Lecciones de Bot. ed. 3, 2: 544. 1939 el nombre Carreto también es empleado en Puerto Berrío (Antioquia. El mismo autor (loc. cit. da el nombre Comulá para una especie indeterminada de Viburnum en Mariquita (Tolima y J. M. Duque, refiriendose a la misma planta y localidad (en Bot. Gen. Colomb. 340, 356. 1943 atribuye este nombre vulgar al Aspidosperma ellipticum Rusby.  Sin embargo, las muestras de madera de Cumulá o Comulá que yo he examinado, procedentes de la región de Mariquita -una de las cuales me fue recientemente enviada por el distinguido ictiólogo Sr. Cecil Miles- pertenecen sin

Can this merger be saved?

Science.gov (United States)

Cliffe, S

1999-01-01

In this fictional case study, a merger that looked like a marriage made in heaven to those at corporate headquarters is feeling like an infernal union to those on the ground. The merger is between Synergon Capital, a U.S. financial-services behemoth, and Beauchamp, Becker & Company, a venerable British financial-services company with strong profits and an extraordinarily loyal client base of wealthy individuals. Beauchamp also boasts a strong group of senior managers led by Julian Mansfield, a highly cultured and beloved patriarch who personifies all that's good about the company. Synergon isn't accustomed to acquiring such companies. It usually encircles a poorly managed turnaround candidate and then, once the deal is done, drops a neutron bomb on it, leaving file cabinets and contracts but no people. Before acquiring Beauchamp, Synergon's macho men offered loud assurances that they would leave the tradition-bound company alone-provided, of course, that Beauchamp met the ambitious target numbers and showed sufficient enthusiasm for cross-selling Synergon's products to its wealthy clients. In charge of making the acquisition work is Nick Cunningham, one of Synergon's more thoughtful executives. Nick, who was against the deal from the start, is the face and voice of Synergon for Julian Mansfield. And Mansfield, in his restrained way, is angry at the constant flow of bureaucratic forms, at the rude demands for instant information, at the peremptory changes. He's even dropping broad hints at retirement. Nick has already been warned: if Mansfield goes, you go. Six commentators advise Nick on how to save his job by bringing peace and prosperity to the feuding couple.

Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

International Nuclear Information System (INIS)

Yarosh, D.B.; Setlow, R.B.

1981-01-01

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity

Manejo Clínico del Oso Palmero (Myrmecophaga tridactyla) bajo Condiciones de Cautiverio en el Bioparque Los Ocarros

OpenAIRE

Dora Adriana Lombo-Rodríguez

2008-01-01

El oso palmero (Myrmecophaga tridactyla. Linnaeus, 1758) es la única especie del género Myrmecophaga. Este mamífero se clasifica dentro del Infraorden: VERMILINGUA, Orden: XENARTHRA. Puede encontrarse en sabanas, pastizales, áreas inundadas y bosques húmedos. Geográficamente se encuentra al Norte desde Costa Rica hasta el Norte de Argentina. En Colombia en los Departamentos de Chocó, Magdalena, Orinoco, Amazonas y los Llanos Orientales....

Potential distribution of Podocnemis lewyana (Reptilia: Podocnemididae) and its possible fluctuation under different global climate change scenarios

International Nuclear Information System (INIS)

Ortiz Yusty, Carlos; Restrepo, Adriana; Paez, Vivian P

2014-01-01

We implemented a species distribution modelling approach to establish the potential distribution of Podocnemis lewyana, to explore the climatic factors that may influence the species' distribution and to evaluate possible changes in distribution under future climate scenarios. The distribution models predicted a continuous distribution from south to north along the Magdalena River, from Rivera and Palermo in the Department of Huila to the departments of Atlantico and Magdalena in the north. Temperature was the variable most influential in the distribution of P. lewyana; this species tends to be present in warm regions with low temperature variability. The distribution model predicted an increase in the geographic range of P. lewyana under climate change scenarios. However, taking into account the habitat preferences of this species and its strong association with water, this result should be treated with caution since the model considered only terrestrial climatic variables. Given the life history characteristics of this species (temperature dependent sex determination, high pivotal temperature and a very narrow transition range) and the negative effect of changes in hydrological regimes on embryo survival, expansion of the potential distribution of P. lewyana in the future does not mean that the species will not be affected by global climate change.

El doctor Moreno Pérez y el anopheles crucians

Directory of Open Access Journals (Sweden)

Facultad de Medicina Revista

1943-03-01

Full Text Available En el año de 1933, con ocasión del conflicto del Amazonas, el doctor Ignacio Moreno·Pérez trabajó como médico de sanidad en la hoya del río Caquetá. Prosiguiendo sus estudios sobre paludismo y mosquitos transmisores a que dedicó gran parte de su meritoria vida, practicó una inspección entomológica de las cercanías de Florencia, capital de la comisaria del Caquetá, y encontró una raza en aguas salobres de Anopheles crucians. El hallazgo comunicado por él en el informe de 1934 de la comisión de paludismo del valle del Magdalena, (Estudios de Paludismo en el Valle del Magdalena, Departamento Nacional de Higiene, Sección de Sanidad Rural, Bogotá editorial de "Cromos", 1934, despertó mucho interés y fué objeto de controversias, porque conforme a los estudios de Howard, Dyar y Knab (1917, la localización del Anopheles crucians era casi hiperbórea, por encima del paralelo 25 en la América del Norte y solamente se había logrado ver además en Cuba y Jamaica.

Briti asepeaminister arvustas Brüsselis võetud positsiooni / Evelyn Kaldoja

Index Scriptorium Estoniae

Kaldoja, Evelyn, 1980-

2011-01-01

Suurbritannia asepeaminister Nick Clegg kritiseeris peaminister David Cameroni seisukohti Euroopa Ülemkogul. Suurbritannia otsusest välja jääda Euroopa Liidu uuest valitsustevahelisest rahanduskokkuleppest

Dos nuevas Bignoniáceas del Valle del Magdalena

Directory of Open Access Journals (Sweden)

Dugand Armando

1942-11-01

Full Text Available Memora Romeroi: La especie aquí descrita pertenece a la sección Eumemora K. Schum. y es muy afín, según descripción, a M. caracasana K. Schum., de Venezuela, pero se distingue principalmente por tener racimos florales mucho más alargados, geminados y axilares, con 8, 12 a más flores opuestas, y por las brácteas infracalicinales de forma anchamente espatulada. Además, el cáliz y la corola son más cortos, particularmente el tubo basilar de esta última. EI raquis foliar y especialmente los peciolulos de M. Romeroi son diminutamente pubérulos, al menos durante mucho tiempo, y esta pubescencia menuda se presenta también en el raquis floral. / Tanaecium exitiosum: El doctor Rafael Mora, colector de esta muy interesante nueva especie, anota lo siguiente: "Arbusto que alcanza una altura de 2 metros; ocasionalmente crece más, arrollándose como bejuco a las otras plantas; las flores son de color blanco y de aspecto tubular alargado. La planta es abundante en las regiones secas de Barranca Bermeja y hay potreros que están completamente invadidos por ella".

Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management

OpenAIRE

Calvete, Juan J.; Sanz, Libia; Pérez, Alicia; Borges, Adolfo; Vargas, Alba M.; Lomonte, Bruno; Angulo, Yamileth; Gutiérrez, José María; Chalkidis, Hipócrates M.; Mourão, Rosa H.V.; Furtado, María de Fátima; Moura Da Silva, Ana M.

2011-01-01

We describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States of Amazonas and Orinoquia, respectively, and São Bento in the Brazilian State of Maranhão. Colombian and Venezuelan venoms show an ontogenetic toxin profile phenotype whereas Brazilian venoms exhibit paedomorphic phenotypes. Venoms from each of the 16 localities sampled cont...

Zehn Oden / Kristjan Jaak Peterson ; [Deutsch von Gisbert Jänicke

Index Scriptorium Estoniae

Peterson, Kristian Jaak, 1801-1822

2001-01-01

On ilmunud ka kogumikus "Iaak. Kristian Jaak Peterson 200" (Tln. : Eesti Keele Sihtasutus, 2001, lk. 105-122). Sisu: Der Mond = Kuu ; Freundschaft = Sõprus ; Der Sänger = Laulja ; An Gott = Jumalale ; Hoffnung = Lootus ; Ich muss Trinken. Liedchen = Ma pean jooma. Lauluke ; Ich bin wieder glücklich = Olen jälle õnnis ; Der Mensch = Inimene ; Sonnenuntergang = Päeva loojaminemine ; Herbst = Sügise.

In the Nick of Time: Proactive Prevention of Obligation Violations

DEFF Research Database (Denmark)

Basin, David; Debois, Søren; Hildebrandt, Thomas

2016-01-01

We present a system model, an enforcement mechanism, and a policy language for the proactive enforcement of timed provisions and obligations. Our approach improves upon existing formalisms in two ways: (1) we exploit the target system's existing functionality to avert policy violations proactively...... declaratively express timed provisions and obligations as causal relationships between events, and DCR states explicitly represent pending obligations. As key technical results, we show that enforceability of DCR policies is decidable, we give a sufficient polynomial time verifiable condition for a policy...... to be enforceable, and we give an algorithm for determining from a DCR state a sequence of actions that discharge impending obligations....

Sequence Classification: 390305 [

Lifescience Database Archive (English)

Full Text Available SOMERASE I TOPA (OMEGA-PROTEIN) (RELAXING ENZYME) (UNTWISTING ENZYME) (SWIVELASE) (TYPE I DNA TOPOISOMERASE) (NICKING-CLOSING ENZYME) || http://www.ncbi.nlm.nih.gov/protein/31794816 ...

Nicholas J. Nagle | NREL

Science.gov (United States)

@nrel.gov | 303-384-6184 Research Interests Nick is interested in understanding how advanced feedstocks Technology (2015) "Acid-catalyzed algal biomass pretreatment for integrated lipid and carbohydrate-based

Size Constraints on Late Miocene to Pliocene Submarine Slope Failures along the Colombian Caribbean Subduction Margin as a Basis for Assessing Circum-Caribbean Impact of Future Tsunami Events

Science.gov (United States)

Leslie, S.; Mann, P.

2015-12-01

The Colombian Caribbean margin provides an ideal setting for the formation of large mass transport deposits (MTDs): 1) the Caribbean Plate is slowly subducting at rates of 20 mm/yr with infrequent large thrust earthquakes and a complete lack of subduction events in the 400-year-long historical record; 2) the margin is a broad zone of active faults including a ~50 km-wide accretionary prism and strike-slip faults landward of the prism; 3) the active margin is draped by the Magdalena delta and submarine fan fed by the Magdalena River, the 26th largest in the world; and 4) the margin is over-steepened to slopes of up to 7° from the combination of tectonic activity and rapid rates of deltaic progradation. Using seismic data we have identified three late Miocene-Pliocene MTDs, the largest of which is between 4500 and 6000 km3, comparable in size to the well-studied Storegga slide of Norway. The tsunamigenic potential of future, analog MTD events are modeled using GeoWave tsunami modeling software. The largest and youngest of these MTDs, the Santa Marta slide, is used as an analog to infer the location and input parameters for the tsunami model. The event is modeled as a translational slide ~46 km long and ~37 km wide with the center of the slide located ~57 km W/NW from the mouth of the present day Magdalena River in water depths of 1500 m. The volume for the initial failure is conservatively estimated at ~680 km3 of material. The resulting tsunami wave from such an event has an initial maximum trough amplitude of -65.8 m and a peak amplitude of 19.2 m. The impact of such a tsunami would include: 1) Kingston, Jamaica (population 938K), tsunami height 7.5 m, peak arrival at 60 min.; 2) Santo Domingo, Dominican Republic (population 965K, height 6 m, peak arrival at 80 min.); and 3) Cartagena, Colombia (population 845K, height 21 m, peak arrival at 34 min.). A number of parameters to the model are varied to analyze sensitivity of modeling results to changes in slide depth

Can physical activity influence the quality of sleep among the elderly?

OpenAIRE

Podhorecka, Marta; Cytarska, Magdalena; Gębka, Dominika; Perkowski, Radosław; Androsiuk-Perkowska, Joanna; Jaroch, Alina; Siedlecka-Główczewska, Emilia; Sokołowski, Remigiusz; Zukow, Walery; Kędziora-Kornatowska, Kornelia

2017-01-01

Podhorecka Marta, Cytarska Magdalena, Gębka Dominika, Perkowski Radosław, Androsiuk-Perkowska Joanna, Jaroch Alina, Siedlecka–Główczewska Emilia, Sokołowski Remigiusz, Zukow Walery, Kędziora-Kornatowska Kornelia. Can physical activity influence the quality of sleep among the elderly? Journal of Education, Health and Sport. 2017;7(12):288-305. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1133730 http://ojs.ukw.edu.pl/index.php/johs/article/view/5177 ...

POW/MIA Issues. Volume 2. World War II and the Early Cold War

Science.gov (United States)

1994-01-01

Norman Edward Caterer, U.S. citizen, born 1920, imprisoned March 27, 1943, detained in Lamsdorf 8b. 2. Lorius Cawcause, U.S. citizen, born 1920, unable...March 29, 1948 27. Magdalena Wormittag #C-86, March 7, 1949 28. Hilda Elizabeta Wagner Letter to Deputy Minister of Foreign Affairs Vyshinsky, March...another winter. Verdine was held in Weimar from the time of his arrest until 1952 then was moved to Lichtenberg prison Berlin. They were separated after

Microorganisms in Soils of Bovine Production Systems in Tropical Lowlands and Tropical Highlands in the Department of Antioquia, Colombia

OpenAIRE

Molina-Guzmán, Licet Paola; Henao-Jaramillo, Paula Andrea; Gutiérrez-Builes, Lina Andrea; Ríos-Osorio, Leonardo Alberto

2018-01-01

Studies on the physical and chemical effects of extensive grazing on soils have been performed in Colombia, but the effects of dairy cattle rearing on the biological properties of soils are not well known. The objective of this study was to evaluate microorganisms in 48 soils from livestock farms in the highland and lowland tropics in the Northern and Magdalena Medio subregions of the Department of Antioquia (Colombia). Principal component analysis demonstrated differences in the edaphic comp...

El género Morphnus

Directory of Open Access Journals (Sweden)

Lehmann V. F. C.

1943-08-01

Full Text Available Nombres comunes: Aguila churuquera, Aguila miquera, Aguila copetona, Gavilán miquero,  Aguila-gavilán, Gavilán. real.  DISTRIBUCION: Desde Honduras, por Panamá, Colombia, Venezuela, las Guayanas, el Brasil, el Oriente del Ecuador y del Perú, hasta el Paraguay. En Colombia se encuentra en las selvas frondosas de las regiones cálidas: Llanos Orientales, Choco, Valle del Magdalena (parte selvosa , sur de la Guajira, Caquetá y Amazonia.

The impact on quality of life of dialysis patients with renal insufficiency

OpenAIRE

Dąbrowska-Bender M; Dykowska G; Żuk W; Milewska M; Staniszewska A

2018-01-01

Marta Dąbrowska-Bender,1 Grażyna Dykowska,2 Wioletta Żuk,3 Magdalena Milewska,1 Anna Staniszewska4 1Department of Clinical Dietetics, Medical University of Warsaw, Warsaw, Poland; 2Department of Public Health, Medical University of Warsaw, Warsaw, Poland; 3Department of Surgery, Transplantation and Extracorporeal Surgery, Medical University of Warsaw, Warsaw, Poland; 4Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland Aim: The aim of the st...

A Novel 3D Hybrid FEM-PO Technique for the Analysis of Scattering Problems

Science.gov (United States)

2004-04-23

Magdalena Salazar-Palma3 Tapan K. Sarkar4 1Departamento de Ingeniería Audiovisual y Comunicaciones , Universidad Politécnica de Madrid 2Departamento de...Teoría de la Señal y Comunicaciones , Universidad de Alcalá Escuela Politécnica, Campus Universitario, Ctra. Madrid-Barcelona Km. 33.600 28806 Alcalá de...NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Departamento de Ingeniería Audiovisual y Comunicaciones , Universidad Politécnica de

Eje Cafetero: fortaleza minero-industrial y posibilidades agropecuarias

OpenAIRE

Duque Escobar, Gonzalo

2012-01-01

Fortalezas del Eje Cafetero relacionadas con sus potencialidades agropecuario y valioso inventario minero, que le dan soporte a los elementos fundamenbtales del Plan Industrial Minero y a nuevas opciones de clústeres regionales agropecuarios, en momentos en los que se perfila un crecimiento de la oferta energética local en el oriente caldense y la implementación de modos de transporte más eficientes como la navegación por el río Magdalena.

Status sistematico del género geobatrachus ruthven 1915 (amphibia: anura)

OpenAIRE

Ardila Robayo, María Cristina

2012-01-01

The different familial assignations proposed until now for Geobatracbus are compiled. Through the collection of a virtual topotypical series of this monotypic genus, which is endemic of the NW section of the Sierra Nevada de Santa Marta Massif (Departamento del Magdalena, Colombia), a detailed description was made.  67 morphological characters were selected according to the criteria used by Lynch and Heyer in their studies on the philogeny and classification of the leptodactylid frogs, and su...

The effect of irradiation on the DNA of cauliflower

International Nuclear Information System (INIS)

Harmey, M.A.

1991-01-01

The cellular DNA is one of the components most affected by ionizing radiation. Lesions caused range from single and double stranded breaks to chemical modification of bases depending on the radiation dosage and the metabolic status of the tissue. In attempting to assess the DNA damage induced by irradiation of vegetables in a speedy and convenient manner, we examined the effect on the DNA by subjecting cauliflower to a dose of 1 kGy. If DNA is nicked by irradiation, the extent of the damage can be assessed by using DNA polymerase to repair the nicks. Comparisons were made between irradiated and non irradiated cauliflower and incorporation of 32 p deoxy GTP in the presence of the Klenow fragment of DNA polymerase measured

Mesozooplankton communities in the Magellan region

Directory of Open Access Journals (Sweden)

Katjia Defren-Janson

1999-12-01

Full Text Available During the Joint Chilean-German-Italian Magellan Victor Hensen Campaign in November 1994 zooplankton sampling was carried out by means of a multiple opening-closing multinet equipped with 300 µm mesh size. Distribution pattern was studied and community analyses of mesozooplankton were made at seven stations in the Magellan region. Highest numbers of individuals were found in the northern part of the investigation area (Magdalena to Brecknock Channels at stations with a mixed water column. In the southern part (Beagle and Ballenero Channels, lower zooplankton abundances were associated with a stratified water column due to melt water from several glaciers. At all stations holoplankton dominated the assemblages (83 - 97%. Copepods were by far the most abundant taxon encountered during this study, contributing to more than 2/3 of the total zooplankton numbers. They occurred throughout the water column with maxima in middle water layers. Appendicularians ranked second in abundance with their main distribution in the upper 100 m. Euphausiids were found in higher densities only in the Magdalena Channel (St. 1313; their vertical distribution pattern resembled that of copepods. Cladocerans aggregated at all stations in the upper 30 m. Within the meroplankton, echinoderm larvae were most abundant, notably in the upper 100 m under stratified conditions. Cluster analysis separated between a surface community covering all stations, and a northern and southern deep community, respectively.

Reports of the hairy-legged vampire bat Diphylla ecaudata Spix, 1823 (Chiroptera: Desmodontinae for the biogeographyc Colombian Chocó

Directory of Open Access Journals (Sweden)

Jonard David Echavarría-R

2017-07-01

Full Text Available It is reported a new record of the hairy-legged vampire Diphylla ecaudata (SPIX, 1823 in the Choco region of Colombia, of which are known only few studies in the country, registered in the departments of Magdalena (1900, Vaupés (1966, Meta (1970, Caquetá (1999 y Amazonas (2016. This registry is based on two specimens that were properly analyzed and due to its curiosity, discusses the available information on this species in the country in terms of its distribution and ecology, based on specimens in collections and existing reports. This log helps fill a void of the known geographic distribution of the species in the country deposited in the Teriologica Scientific Collection Research Group on Management and Wildlife Management of the Chocó of the Technological University of Chocó (ColTer-Choco. This report extends the geographic distribution until the department of Choco, in contraendose to 464.794 km some of the record closest in Magdalena, adding the Tropical Dry Forest Ecosystem (BST to habitats that occurred for the species. Due to its curiosity, discusses the available information on this species in the country in terms of its distribution and ecology, based on specimens in collections and existing reports. This log helps fill a void of the known geographic distribution of the species in the country.

Mitochondrial and nuclear genetic variation across calving lagoons in Eastern North Pacific gray whales (Eschrichtius robustus).

Science.gov (United States)

Alter, S Elizabeth; Ramirez, Sergio Flores; Nigenda, Sergio; Ramirez, Jorge Urbán; Bracho, Lorenzo Rojas; Palumbi, Stephen R

2009-01-01

Accurate knowledge of population structure in cetaceans is critical for preserving and managing breeding habitat, particularly when habitat is not uniformly protected. Most eastern gray whales return to their major breeding range each winter along the Pacific coast of Baja California, Mexico, concentrating in 3 major calving lagoons, but it is unknown whether genetic differences exist between lagoons. Previous photo-identification studies and genetic studies suggest that gray whales may return to their natal lagoons to breed, potentially resulting in the buildup of genetic differences. However, an earlier genetic study used only one genetic marker and did not include samples from Bahia Magdalena, a major calving lagoon not currently designated as a wildlife refuge. To expand on this previous study, we collected genetic data from the mitochondrial control region (442 bp) and 9 microsatellite markers from 112 individuals across all 3 major calving lagoons. Our data suggest that migration rates between calving lagoons are high but that a small but significant departure from panmixia exists between Bahia Magdalena and Laguna San Ignacio (Fisher's Exact test, P 10% per generation). In addition, microsatellite data showed evidence of a severe population bottleneck. Eastern gray whales are still recovering from the impacts of whaling on their breeding grounds, and these populations should be protected and monitored for future genetic changes.

Approaches for the environmental evaluation of two swamp complexes of the Momposina depression

International Nuclear Information System (INIS)

Caballero, Humberto; Durango, Consuelo

1998-01-01

The swamp complexes of the Momposina depression in the lower Magdalena River have been submitted to irrational exploitation, transformation and contamination mainly since the beginning of the century, process that has be en getting worse in the last decade. The most evident manifestation of the environmental deterioration is expressed by the drastic reduction of fishing, with important socio-economic effects to the population that lives from this source. The most notorious causes of the actual state of the ecosystem were detected, and some preliminary considerations for its management are presented. However, due to the modifications that the complexes have suffered (closure of the natural sewer, construction of jarillones, modifications of the internal water flows and sediments, over exploitation, of fish and herpetofauna), the deterioration of the limnologic conditions is moderated, which allows to begin recuperation plans. The major impacts were observed in the fauna of birds and reptiles associated to the swamps; the decrease of the ictics populations seems to be related to the strong impact generated by the fishing action used and the interruption of the natural cycles of fish. The possibility of rebuilding the communication swamp-river should be studied with care because it seems the system has arrived to new conditions. The re-opening of the sewers could mean a new unbalanced condition represented mainly by the contamination coming from the Magdalena River

Assessment of water supply as an ecosystem service in a rural-urban watershed in southwestern Mexico City.

Science.gov (United States)

Jujnovsky, Julieta; González-Martínez, Teresa Margarita; Cantoral-Uriza, Enrique Arturo; Almeida-Leñero, Lucia

2012-03-01

Studies from the ecosystem services perspective can provide a useful framework because they allow us to fully examine the benefits that humans obtain from socio-ecological systems. Mexico City, the second largest city in the world, has faced severe problems related to water shortages, which have worsened due to increasing population. Demand for space has forced changes in land cover, including covering areas that are essential for groundwater recharge. The city has 880 km(2) of forest areas that are crucial for the water supply. The Magdalena River Watershed was chosen as a model because it is a well-preserved zone within Mexico City and it provides water for the population. The general aim of this study was to assess the ecosystem service of the water supply in the Magdalena River Watershed by determining its water balance (SWAT model) and the number of beneficiaries of the ecosystem services. The results showed that the watershed provides 18.4 hm(3) of water per year. Baseflow was dominant, with a contribution of 85%, while surface runoff only accounted for 15%. The zone provides drinking water to 78,476 inhabitants and could supply 153,203 potential beneficiaries. This work provides an example for understanding how ecosystem processes determine the provision of ecosystem services and benefits to the population in a rural-urban watershed in Mexico City.

76 FR 9071 - Advisory Committee on Historical Diplomatic Documentation; Notice of Meeting

Science.gov (United States)

2011-02-16

... notify Nick Sheldon, Office of the Historian (202-663-1123) no later than March 3, 2011 to provide date..., Advisory Committee on Historical Diplomatic Documentation, Department of State, Office of the Historian...

Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli DNA Ligase (LigA).

Science.gov (United States)

Wang, Li Kai; Zhu, Hui; Shuman, Stewart

2009-03-27

NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria, where they are essential for growth and present attractive targets for antimicrobial drug discovery. LigA has a distinctive modular structure in which a nucleotidyltransferase catalytic domain is flanked by an upstream NMN-binding module and by downstream OB-fold, zinc finger, helix-hairpin-helix, and BRCT domains. Here we conducted a structure-function analysis of the nucleotidyltransferase domain of Escherichia coli LigA, guided by the crystal structure of the LigA-DNA-adenylate intermediate. We tested the effects of 29 alanine and conservative mutations at 15 amino acids on ligase activity in vitro and in vivo. We thereby identified essential functional groups that coordinate the reactive phosphates (Arg(136)), contact the AMP adenine (Lys(290)), engage the phosphodiester backbone flanking the nick (Arg(218), Arg(308), Arg(97) plus Arg(101)), or stabilize the active domain fold (Arg(171)). Finer analysis of the mutational effects revealed step-specific functions for Arg(136), which is essential for the reaction of LigA with NAD(+) to form the covalent ligase-AMP intermediate (step 1) and for the transfer of AMP to the nick 5'-PO(4) to form the DNA-adenylate intermediate (step 2) but is dispensable for phosphodiester formation at a preadenylylated nick (step 3).

Plaadid / Villu Koskaru

Index Scriptorium Estoniae

Koskaru, Villu, 1976-

2003-01-01

Uutest plaatidest Era "The Mass", Dannii Minogue "Neon Nights", Aaliyah "I Care 4 U", Linkin Park " Meteora", "Smash Hits 6", The Mighty Pop "Yellow", John Taylor "Rosslyn", Nick Cave & The Bad Seds "Nocturama"

Cavernous hemangioma of the knee - case report

OpenAIRE

Weiss, Marcin; Dolata, Tomasz; Weiss, Waldemar; Maksymiak, Martyna; Kałużny, Krystian; Kałużna, Anna; Zukow, Walery; Hagner Derengowska, Magdalena

2018-01-01

Weiss Marcin, Dolata Tomasz, Weiss Waldemar, Maksymiak Martyna, Kałużny Krystian, Kałużna Anna, Zukow Walery, Hagner‑Derengowska Magdalena. Cavernous hemangioma of the knee - case report. Journal of Education, Health and Sport. 2018;8(4):318-325. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1226645 http://ojs.ukw.edu.pl/index.php/johs/article/view/5438 The journal has had 7 points in Ministry of Science and Higher Education parametric evalu...

Treatment of intraarticular displaced fractures of the calcaneus bone using nail blocked calcanail

OpenAIRE

Weiss, Marcin; Dolata, Tomasz; Weiss, Waldemar; Maksymiak, Martyna; Kałużny, Krystian; Kałużna, Anna; Zukow, Walery; Hagner‑Derengowska, Magdalena

2018-01-01

Weiss Marcin, Dolata Tomasz, Weiss Waldemar, Maksymiak Martyna, Kałużny Krystian, Kałużna Anna, Zukow Walery, Hagner‑Derengowska Magdalena. Treatment of intraarticular displaced fractures of the calcaneus bone using nail blocked calcanail. Journal of Education, Health and Sport. 2018;8(4):338-345. eISNN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1226782 http://ojs.ukw.edu.pl/index.php/johs/article/view/5439 https://pbn.nauka.gov.pl/sedno-webapp/works/863609 ...

Raport z badania 2016. Specjalne Strefy Ekonomiczne w Polsce w oczach przedsiębiorców i pracowników samorządów

OpenAIRE

Pastusiak, Radosław; Jasiniak, Magdalena; Keller, Jakub; Krzeczewski, Bartłomiej

2016-01-01

Niniejszy raport prezentuje wyniki projektu realizowanego w ramach projektu badawczego nr UMO-2013/09/B/HS4/, pt. Efektywność Specjalnych Stref Ekonomicznych, przez zespół Katedry Finansów Korporacji, Wydział Ekonomiczno-Socjologiczny Uniwersytetu Łódzkiego, pod kierownictwem dr hab. Radosława Pastusiaka, prof. nadzw. UŁ. w składzie: dr Magdalena Jasiniak, mgr Jakub Keller, mgr Bartłomiej Krzeczewski. Zasadniczym celem badania było określenie efektywności funkcjonowania Specjalnych Stref Ekon...

Soil moisture retention and mass movement of volcanic soils from the “Sabinas” sector in Caldas, Colombia

OpenAIRE

William Chavarriaga Montoya; Josè Gabriel Cruz Cerón; Johan Cuervo Correa

2017-01-01

The mass movement of soils, are soil, rocks displacements or both, caused by soil water excesses in terrains due to gravity effects and other factors. The aim of this research was to quantify the moisture retention capacity of volcanic soils as a threat indicator in the mass removal phenomena. This research was carried out on soils of the Malteria - Las Margaritas road transept to Magdalena river, right bank of the Chinchiná river in the Department of Caldas, Colombia. Through soil sample des...

Holistyczne kompetencje zawodowe studentów pielęgniarskich studiów magisterskich = The holistic nursing professional competence of students graduate

OpenAIRE

Brodowicz-Król, Magdalena; Zarzycka, Danuta; Stadnicka, Sabina; Bartoń, Elżbieta

2016-01-01

Brodowicz-Król Magdalena, Zarzycka Danuta, Stadnicka Sabina, Bartoń Elżbieta. Holistyczne kompetencje zawodowe studentów pielęgniarskich studiów magisterskich = The holistic nursing professional competence of students graduate. Journal of Education, Health and Sport. 2016;6(8):113-127. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.59880 http://ojs.ukw.edu.pl/index.php/johs/article/view/3736 The journal has had 7 points in Ministry of Science and Higher Educat...

Colombia: Emerging Labor Movement and the State Colombian Drug Problem: Effect on the Peace Process between the M19 and the State

Science.gov (United States)

1990-05-01

de la Energia en Colombia. 1537-1930 (Bogota,1985), 1. 39 Steam navigation in the inland rivers, Magdalena and Cauca Rivers, used wood as a fuel to...Cartagena, 325. 72 Table 5. Cartagena-Oil Refining Co. 1912 Prod Rates (Monthly) ° Product Octane Amount Gasoline 72 Kerosene "Luz Solar " 110 Kerosene "Luz...1987. De La Pedraja, Ren6. Energy Politics in Colombia, San Francisco : Westview Press, 1989. De La Pedraja, Ren6. Historia de la Energia en Colombia

Jałowe martwice kości, charakterystyka choroby i postępowanie fizykalne – przegląd literatury = Sterile necrosis of bone, the characteristics of the illness and physical examination - a literature review

OpenAIRE

Radzimińska, Agnieszka; Zimmermann, Agnieszka Anna; Weber-Rajek, Magdalena; Strojek, Katarzyna; Bułatowicz, Irena; Goch, Aleksander; Zukow, Walery

2015-01-01

Radzimińska Agnieszka, Zimmermann Agnieszka Anna, Weber-Rajek Magdalena, Strojek Katarzyna, Bułatowicz Irena, Goch Aleksander, Zukow Walery. Jałowe martwice kości, charakterystyka choroby i postępowanie fizykalne – przegląd literatury = Sterile necrosis of bone, the characteristics of the illness and physical examination - a literature review. Journal of Education, Health and Sport. 2015;5(10):108-120. ISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.32518 http://ojs.ukw.edu.pl/index....

75 FR 71787 - Advisory Committee On Historical Diplomatic Documentation; Notice of Meeting

Science.gov (United States)

2010-11-24

... session must notify Nick Sheldon, Office of the Historian (202-663-1123) no later than December 9, 2010... of State, Office of the Historian, Washington, DC 20520, telephone (202) 663-1123, (e-mail history...

76 FR 53165 - Advisory Committee on Historical Diplomatic Documentation; Notice of Meeting

Science.gov (United States)

2011-08-25

... planning to attend must notify Nick Sheldon, Office of the Historian (202-663-1123) no later than September... of State, Office of the Historian, Washington, DC 20520, telephone (202) 663-1123, (e-mail history...

Pop / Tauno Maarpuu

Index Scriptorium Estoniae

Maarpuu, Tauno

2006-01-01

Heliplaatidest: Scandinavian Music Group "Hölmö rakkaus", Baron Zen "At The Mall", Cave, Nick & Warren Ellis "The Proposition. Original Soundtrack", Eläkeläiset "Humppasirkus", Warren Zevon "Reconsider Me: The Love Songs"

Vene elektrivõrk : Gazprom on ebakindel gaasitarnija / Raimo Poom

Index Scriptorium Estoniae

Poom, Raimo

2007-01-01

Tallinnas toimunud rahvusvahelisel energiajulgeoleku konverentsil räägiti energia- ja keskkonnateemadel. Vene elektrivõrkude esindaja Marina Liborakina hoiatas Gazpromi vigade eest gaasi tarnimisel. Kommenteerib Briti nõustamisfirma E3G juhataja Nick Mabey

Plaadid / Rein Vader, Heidi Purga, Tiiu Laks...[jt.

Index Scriptorium Estoniae

2008-01-01

Uutest heliplaatidest Nick Cave & The Bad Seeds "Dig, Lazarus, Dig!", Erykah Badu "New Amerikah Part One", Leslie da Bass "Nights By Open Windows", Jack Johnson "Sleep Througth The Static", Janet Jackson "Discipline", "Rock & Roll City"

Will we ever see their like again? Departing directors at the top US physics laboratories leave a discipline in search of inspired leadership

CERN Multimedia

1998-01-01

Burt Richter stands down next August as director of the Stanford Linear Accelerator Centre. His departure follows that of Nick Samios from Brookhaven National Laboratory and the retirement next year of John Peoples from Fermilab.

Pop / Mart Juur

Index Scriptorium Estoniae

Juur, Mart, 1964-

2005-01-01

Heliplaatidest: Nick Cave & The Bad Seeds "B-Sides & Rarities", Koneveljet "Hoax", Everything But The Girl "Adapt or Die. Ten Years of Remixes", Autechre "Untitled", Daedelus "Exquisite Corpse", Keane "Live recordings 2004", Iris Garrelfs "Specified Encounters"

Electric Six : Ettevaatust! Kõrgepinge!

Index Scriptorium Estoniae

2007-01-01

Ameerika rockansamblist Electric Six, albumitest "Fire", "Swizerland". Ameerika rockauljast-kitarristist Nick Oliveri'st, ansambli Mondo Generator (www.mondogenerator.com) albumist "Dead Planet: SonicSlowMotionTrails". Info festivalist: www.rabarock.delfi.ee / www.rabarock.ee

Harbor porpoise - Passive acoustic monitoring of harbor porpoises in Puget Sound

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Drs. Marla Holt, Brad Hanson, and Candice Emmons of the NWFSC, along with efforts from the NWFSC dive team (coordinated by Nick Tolimieri), are currently conducting...

Nick Jonas on Type 1 Diabetes | NIH MedlinePlus the Magazine

Science.gov (United States)

Skip to main content NIH MedlinePlus the Magazine NIH MedlinePlus Salud Download the Current Issue PDF [1.9 mb] Trusted Health Information from the National Institutes of Health Home Current Issue ...

Výzkum diváka Strašnického divadla

OpenAIRE

Humlová, Jana

2012-01-01

This bachelor thesis focuses on marketing applied by theatres. Firstly there is a definition of marketing in general and then the thesis focuses on differences in marketing applied by cultural organizations, especially by theatres. Then it shows diverse types of theatres. Afterwards the thesis becomes less theoretical and gets more concrete and it explains the term marketing mix on example of Strašnice theatre. The practical part is represented by a marketing research. The purpose of the rese...

Processing of Nonconjugative Resistance Plasmids by Conjugation Nicking Enzyme of Staphylococci

Energy Technology Data Exchange (ETDEWEB)

Pollet, Rebecca M.; Ingle, James D.; Hymes, Jeff P.; Eakes, Thomas C.; Eto, Karina Yui; Kwong, Stephen M.; Ramsay, Joshua P.; Firth, Neville; Redinbo, Matthew R. (Curtin U.); (Sydney); (UNC)

2016-01-04

Antimicrobial resistance inStaphylococcus aureuspresents an increasing threat to human health. This resistance is often encoded on mobile plasmids, such as pSK41; however, the mechanism of transfer of these plasmids is not well understood. In this study, we first examine key protein-DNA interactions formed by the relaxase enzyme, NES, which initiates and terminates the transfer of the multidrug resistance plasmid pSK41. Two loops on the NES protein, hairpin loops 1 and 2, form extensive contacts with the DNA hairpin formed at theoriTregion of pSK41, and here we establish that these contacts are essential for proper DNA cleavage and religation by the full 665-residue NES proteinin vitro. Second, pSK156 and pCA347 are nonconjugativeStaphylococcus aureusplasmids that contain sequences similar to theoriTregion of pSK41 but differ in the sequence predicted to form a DNA hairpin. We show that pSK41-encoded NES is able to bind, cleave, and religate theoriTsequences of these nonconjugative plasmidsin vitro. Although pSK41 could mobilize a coresident plasmid harboring its cognateoriT, it was unable to mobilize plasmids containing the pSK156 and pCA347 variantoriTmimics, suggesting that an accessory protein like that previously shown to confer specificity in the pWBG749 system may also be involved in transmission of plasmids containing a pSK41-likeoriT. These data indicate that the conjugative relaxase intransmechanism recently described for the pWBG749 family of plasmids also applies to the pSK41 family of plasmids, further heightening the potential significance of this mechanism in the horizontal transfer of staphylococcal plasmids.

IMPORTANCEUnderstanding the mechanism of antimicrobial resistance transfer in bacteria such asStaphylococcus aureusis an important step toward potentially slowing the spread of antimicrobial-resistant infections. This work establishes protein-DNA interactions essential for the transfer of theStaphylococcus aureusmultiresistance plasmid pSK41 by its relaxase, NES. This enzyme also processed variantoriT-like sequences found on numerous plasmids previously considered nontransmissible, suggesting that in conjunction with an uncharacterized accessory protein, these plasmids may be transferred horizontally via a relaxase intransmechanism. These findings have important implications for our understanding of staphylococcal resistance plasmid evolution.

Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction

Czech Academy of Sciences Publication Activity Database

Ménová, Petra; Hocek, Michal

2012-01-01

Roč. 48, č. 55 (2012), s. 6921-6923 ISSN 1359-7345 R&D Projects: GA ČR GA203/09/0317 Institutional support: RVO:61388963 Keywords : cross - coupling reactions * nucleoside triphosphates * functionalized DNA * restriction endonucleases * polymerase incorporation Subject RIV: CC - Organic Chemistry Impact factor: 6.378, year: 2012

Dipmeter paleocurrent determination in the Provincia Field, Middle Magdalena Valley

International Nuclear Information System (INIS)

Rubiano, J.L.

1998-01-01

For the determination of the paleocurrent directions in the Provinica Field, were took 2664 measurements of the dip meter logs were taken to make the statistical analyses of the Formations that are between Umir and the Real Group (Maastrichtian and Miocene age). The paleocurrent directions are defined based on statistics analyses, as the average (in azimuth) and its consistency ratio (CR). The paleocurrent directions are principally to the northeast for the Umir und the Lower Real Formations; to the east for the Lisama, Esmeraldas, Mugrosa, Colorado and Middle Real Formations, and in the southeast for the Upper Real Formation. In general, there were observed important changes in the paleocurrent directions between the Umir-Lisama, Lisama-Emeralds, Colorado - Real and between the medium and the upper part of the Real Group. These changes in the paleocurrent directions suggest being associated to tectonic events that involved this area

EL ALTO MAGDALENA- COLOMBIA DE LA MANO CON ENERGIAS ALTERNATIVAS

Directory of Open Access Journals (Sweden)

Barragán-Alturo, Ancízar

2013-12-01

Full Text Available El afán por destruir un paradigma, que mantiene encadenados a los habitantes de Girardot y la región, a una compañía de distribución de la energía eléctrica con sus altos precios para el kilowatt-hora, ha inspirado la investigación CUNDINAMARCA DE LA MANO DE LAS ENERGIAS ALTERNATIVAS, demostrando por diversos caminos que el montaje de paneles solares para generación de energía eléctrica en las cubiertas de las casas es la energía alternativa para la solución de diversos problemas, entre ellos: los costos elevados, las fluctuaciones de voltaje, los cortes de energía, los daños en los electrodomésticos

RBS and SEM analysis of the nickel-fullerene hybrid systems

Czech Academy of Sciences Publication Activity Database

Vacík, Jiří; Naramoto, J.; Narumi, K.; Yamanoto, S.; Abe, J.

2003-01-01

Roč. 206, - (2003), s. 395-398 ISSN 0168-583X Institutional research plan: CEZ:AV0Z1048901 Keywords : nicke-fullerene hybrid Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.041, year: 2003

Book Review: A Liberal Actor in a Realist World the European Union ...

African Journals Online (AJOL)

Abstract. Book Title: A Liberal Actor in a Realist World the European Union Regulatory State and the Global Political Economy of Energy. Book Author: Andreas Goldthau & Nick Sitter. Oxford University Press Oxford 2015. ISBN 9780198719595 ...

Wpływ rezonansu stochastycznego na równowagę dziecka z Mózgowym Porażeniem Dziecięcym = Influence of stochastic resonance on body balance of children with Cerebral Palsy

OpenAIRE

Srokowski, Grzegorz; Radzimińska, Agnieszka; Weber-Rajek, Magdalena; Piekorz, Zuzanna; Siedlaczek, Marcin; Jasionek, Agnieszka; Srokowska, Anna; Zukow, Walery

2015-01-01

Srokowski Grzegorz, Radzimińska Agnieszka, Weber-Rajek Magdalena, Piekorz Zuzanna, Siedlaczek Marcin, Jasionek Agnieszka, Srokowska Anna, Zukow Walery. Wpływ rezonansu stochastycznego na równowagę dziecka z Mózgowym Porażeniem Dziecięcym = Influence of stochastic resonance on body balance of children with Cerebral Palsy. Journal of Education, Health and Sport. 2015;5(12):521-534. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.42266 http://ojs.ukw.edu.pl/index.php/johs/article/view/...

Voces y silencios: paradojas de la Virgen

OpenAIRE

Solarte, Roberto

2017-01-01

El texto enfrenta el fenómeno de la Virgen del Carmen en la experiencia narrada por las comunidades del Magdalena Medio y reconstruye el imaginario religioso de esa celebración para exponer su construcción paradójica, siempre bajo el signo de la violencia. De esta manera, busca la identificación de la experiencia religiosa en toda su ambigüedad, con el fin de delimitar posibilidades de experiencia de fe, comprendida como construcciones de vida por medios no-violentos.

Stosowanie zabiegów fizjoterapeutycznych u osób uprawiających balet po urazie sportowym oraz przeciążeniach narządu ruchu = The use of physical therapy in people practicing ballet trauma and sports overload the musculoskeletal system

OpenAIRE

Michalczak, Magdalena; Bojarczuk, Katarzyna; Smoleń, Agata

2016-01-01

Michalczak Magdalena, Bojarczuk Katarzyna, Smoleń Agata. Stosowanie zabiegów fizjoterapeutycznych u osób uprawiających balet po urazie sportowym oraz przeciążeniach narządu ruchu = The use of physical therapy in people practicing ballet trauma and sports overload the musculoskeletal system. Journal of Education, Health and Sport. 2016;6(7):640-651. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.60867 http://ojs.ukw.edu.pl/index.php/johs/article/view/3783 The j...

76 FR 28844 - Advisory Committee on Historical Diplomatic Documentation; Notice of Meeting

Science.gov (United States)

2011-05-18

... notify Nick Sheldon, Office of the Historian (202-663-1123) no later than June 2, 2011 to provide date of..., Department of State, Office of the Historian, Washington, DC 20520, telephone (202) 663-1123, (e-mail history...

Folk, akustiline selline, on hambus Tristan Priimäel / Tristan Priimägi

Index Scriptorium Estoniae

Priimägi, Tristan, 1976-

2004-01-01

Heliplaatidest: Iron & Wine "Our Endless Numbered Days", Sufjan Stevens "Seven Swans", Joanna Newsom "The Milk-Eyed Mender", Nick Drake "Made to Love Magic", Kings Of Convenience "Riot On an Empty Street", White Magic "Through the Sun Door"

Application of DNA Machineries for the Barcode Patterned Detection of Genes or Proteins.

Science.gov (United States)

Zhou, Zhixin; Luo, Guofeng; Wulf, Verena; Willner, Itamar

2018-06-05

The study introduces an analytical platform for the detection of genes or aptamer-ligand complexes by nucleic acid barcode patterns generated by DNA machineries. The DNA machineries consist of nucleic acid scaffolds that include specific recognition sites for the different genes or aptamer-ligand analytes. The binding of the analytes to the scaffolds initiate, in the presence of the nucleotide mixture, a cyclic polymerization/nicking machinery that yields displaced strands of variable lengths. The electrophoretic separation of the resulting strands provides barcode patterns for the specific detection of the different analytes. Mixtures of DNA machineries that yield, upon sensing of different genes (or aptamer ligands), one-, two-, or three-band barcode patterns are described. The combination of nucleic acid scaffolds acting, in the presence of polymerase/nicking enzyme and nucleotide mixture, as DNA machineries, that generate multiband barcode patterns provide an analytical platform for the detection of an individual gene out of many possible genes. The diversity of genes (or other analytes) that can be analyzed by the DNA machineries and the barcode patterned imaging is given by the Pascal's triangle. As a proof-of-concept, the detection of one of six genes, that is, TP53, Werner syndrome, Tay-Sachs normal gene, BRCA1, Tay-Sachs mutant gene, and cystic fibrosis disorder gene by six two-band barcode patterns is demonstrated. The advantages and limitations of the detection of analytes by polymerase/nicking DNA machineries that yield barcode patterns as imaging readout signals are discussed.

Method for introducing unidirectional nested deletions

Science.gov (United States)

Dunn, J.J.; Quesada, M.A.; Randesi, M.

1999-07-27

Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment. More specifically, the method comprises providing a recombinant DNA construct comprising a DNA segment of interest inserted in a cloning vector. The cloning vector has an f1 endonuclease recognition sequence adjacent to the insertion site of the DNA segment of interest. The recombinant DNA construct is then contacted with the protein pII encoded by gene II of phage f1 thereby generating a single-stranded nick. The nicked DNA is then contacted with E. coli Exonuclease III thereby expanding the single-stranded nick into a single-stranded gap. The single-stranded gapped DNA is then contacted with a single-strand-specific endonuclease thereby producing a linearized DNA molecule containing a double-stranded deletion corresponding in size to the single-stranded gap. The DNA treated in this manner is then incubated with DNA ligase under conditions appropriate for ligation. Also disclosed is a method for producing single-stranded DNA probes. In this embodiment, single-stranded gapped DNA, produced as described above, is contacted with a DNA polymerase in the presence of labeled nucleotides to fill in the gap. This DNA is then linearized by digestion with a restriction enzyme which cuts outside the DNA segment of interest. The product of this digestion is then denatured to produce a labeled single-stranded nucleic acid probe. 1 fig.

Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency.

Science.gov (United States)

Taylor, Mark R; Conrad, John A; Wahl, Daniel; O'Brien, Patrick J

2011-07-01

DNA ligase I (LIG1) catalyzes the ligation of single-strand breaks to complete DNA replication and repair. The energy of ATP is used to form a new phosphodiester bond in DNA via a reaction mechanism that involves three distinct chemical steps: enzyme adenylylation, adenylyl transfer to DNA, and nick sealing. We used steady state and pre-steady state kinetics to characterize the minimal mechanism for DNA ligation catalyzed by human LIG1. The ATP dependence of the reaction indicates that LIG1 requires multiple Mg(2+) ions for catalysis and that an essential Mg(2+) ion binds more tightly to ATP than to the enzyme. Further dissection of the magnesium ion dependence of individual reaction steps revealed that the affinity for Mg(2+) changes along the reaction coordinate. At saturating concentrations of ATP and Mg(2+) ions, the three chemical steps occur at similar rates, and the efficiency of ligation is high. However, under conditions of limiting Mg(2+), the nick-sealing step becomes rate-limiting, and the adenylylated DNA intermediate is prematurely released into solution. Subsequent adenylylation of enzyme prevents rebinding to the adenylylated DNA intermediate comprising an Achilles' heel of LIG1. These ligase-generated 5'-adenylylated nicks constitute persistent breaks that are a threat to genomic stability if they are not repaired. The kinetic and thermodynamic framework that we have determined for LIG1 provides a starting point for understanding the mechanism and specificity of mammalian DNA ligases.

Unique Helicase Determinants in the Essential Conjugative TraI Factor from Salmonella enterica Serovar Typhimurium Plasmid pCU1

Energy Technology Data Exchange (ETDEWEB)

McLaughlin, Krystle J.; Nash, Rebekah P.; Redinbo, Mathew R. (UNC)

2014-06-16

The widespread development of multidrug-resistant bacteria is a major health emergency. Conjugative DNA plasmids, which harbor a wide range of antibiotic resistance genes, also encode the protein factors necessary to orchestrate the propagation of plasmid DNA between bacterial cells through conjugative transfer. Successful conjugative DNA transfer depends on key catalytic components to nick one strand of the duplex DNA plasmid and separate the DNA strands while cell-to-cell transfer occurs. The TraI protein from the conjugative Salmonella plasmid pCU1 fulfills these key catalytic roles, as it contains both single-stranded DNA-nicking relaxase and ATP-dependent helicase domains within a single, 1,078-residue polypeptide. In this work, we unraveled the helicase determinants of Salmonella pCU1 TraI through DNA binding, ATPase, and DNA strand separation assays. TraI binds DNA substrates with high affinity in a manner influenced by nucleic acid length and the presence of a DNA hairpin structure adjacent to the nick site. TraI selectively hydrolyzes ATP, and mutations in conserved helicase motifs eliminate ATPase activity. Surprisingly, the absence of a relatively short (144-residue) domain at the extreme C terminus of the protein severely diminishes ATP-dependent strand separation. Collectively, these data define the helicase motifs of the conjugative factor TraI from Salmonella pCU1 and reveal a previously uncharacterized C-terminal functional domain that uncouples ATP hydrolysis from strand separation activity.

Briti publik armastab King Kongi / Andris Feldmanis

Index Scriptorium Estoniae

Feldmanis, Andris, 1982-

2006-01-01

Briti filmiajakiri Empire lugejate filmiauhinnad : parim film "King Kong" (Peter Jackson), parim režii Steve Box, Nick Park "Wallace & Gromit - libaküüliku needus", parim meesnäitleja Johnny Depp, parim naisnäitleja Thandie Newton

78 FR 67927 - Foreign Trade Regulations (FTR): Mandatory Automated Export System Filing for All Shipments...

Science.gov (United States)

2013-11-13

... Internet at [email protected] ). FOR FURTHER INFORMATION CONTACT: Nick Orsini, Chief, Foreign Trade Division... 0607-AA50 Foreign Trade Regulations (FTR): Mandatory Automated Export System Filing for All Shipments... approval of new information collection requirements. [[Page 67928

Wallace ja Gromit tulid Londonis linale / Andres Laasik

Index Scriptorium Estoniae

Laasik, Andres, 1960-2016

2005-01-01

Londonis esilinastus animafilm "Wallace ja Gromit : Libaküüliku needus" : režissöör Nick Park : Suurbritannia 2005. Kohal olid ka tegelastele hääle andnud Ralph Fiennes, Helena Bonham Carter ja Peter Sallis

Pre-Processing Data Using Wavelet Transform and PCA Based on ...

Indian Academy of Sciences (India)

5

wavelet theory in the last century, using this tool in the hydrology has been an ... These models are based on Statistical Learning Theory, which is ...... Hutcheson G and Nick S 1999 The multivariate social scientist: Introductory statistics using.

"Of comics and men: A cultural history of American comic books," by Jean-Paul Gabilliet

Directory of Open Access Journals (Sweden)

Drew Morton

2013-06-01

Full Text Available Jean-Paul Gabilliet. Of comics and men: A cultural history of American comic books. Translated by Bart Beaty and Nick Nguyen. Jackson: University Press of Mississippi, 2010, hardcover, $55.00 (390p, ISBN 978-1604732672.

Pop / Siim Nestor

Index Scriptorium Estoniae

Nestor, Siim, 1974-

2008-01-01

Heliplaatidest: Janet Jackson "Discipline", Jack Johnson "Sleep Through The Static", The Mars Volta "The Bedlam in Goliath", Christopher Clark "Turning Dragon", Erykah Badu "New Amerykah Part One (4th World War)", Nick Cave & The Bad Seeds "Dig, Lazarus, Dig!!!"

Uus peaminister, uudne valitsus / Kaivo Kopli

Index Scriptorium Estoniae

Kopli, Kaivo

2010-01-01

Suurbritannia sai koalitsioonivalitsuse. Nii peaminister David Cameron kui ka asepeaminister Nick Clegg on vihjanud, et eelistavad lõdvemaid suhteid USA-ga, kui need olid Gordon Brownil ja Tony Blairil. Konservatiivide partei on skeptiline EL-i koostöö suhtes

Uusi plaate

Index Scriptorium Estoniae

2003-01-01

Heliplaatidest: Tom Jones "Greatest hits", Pet Shop Boys "Disco 3", Epoca De Ouro Ensemble "Cafe Brasil 2", Nick Cave and The Bad Seeds "Nocturama", Pistepirkko "The Nature of 22 Pistepirkko", Jimi Tenor "Higher Planes", "Guitars, Saxes & More", "Inspired 2"

Pop / Mart Kuldkepp

Index Scriptorium Estoniae

Kuldkepp, Mart

2008-01-01

Heliplaatidest: The Wombats "A Guide to Love, Loss & Desperation", Lenny Kravitz "It is Time for a Love Revolution", Nick Cave & Warren Ellis "OST: The Assassination of Jesse James by the Coward Robert Ford", Pat Metheny "Secret Story", Lupe Fiasco "The Cool"

A new protocol for extraction of C0t-1 DNA from rice

African Journals Online (AJOL)

USER

2010-07-12

Jul 12, 2010 ... plant species differentiation and genome evolution. A new protocol to ... According to nick translation theory, the genomic DNA was digested by DNase I and ... according to the formula Cot-1 = 1 = mol/L × Ts. Since highly and.

Fulltext PDF

Indian Academy of Sciences (India)

In the first group, the paper by Nick Devereux reviews Hubble Space .... here that the STARK-B database is included in the new search facility for such data namely Virtual Atomic and Molecular Data Center (VAMDC – http://vamdc.org/).

Pop & rock / Valner Valme

Index Scriptorium Estoniae

Valme, Valner, 1970-

2001-01-01

Uute heliplaatide: Nick Cave And The Bad Seeds "No More Shall We Part", Andru Donalds "Let's Talk About It", No Angels "Elle'ments", The Experimental Pop Band "The Tracksuit Trilogy", Ellen ten Damme "I Am Here", Id_Rev "Sina ei" tutvustused

Plaadid / Villu Päärt

Index Scriptorium Estoniae

Päärt, Villu, 1972-

2002-01-01

Uutest plaatidest Nick Carter "Now Or Never", Brian Mcknight "From There To here 1989-2002", Rod Stuart "The Grat American Songbook", New Order "International", Justin Timberlake "Justified", Manic Street Prachers "Forever Delayed - Greatest Hits", LHB "Tell 'Em Who We Are"

Äraigatsuse salakajad / Aita Kivi

Index Scriptorium Estoniae

Kivi, Aita, 1954-

2001-01-01

Sisu : Rein Põder. Hula; David Shahar. Tema majesteedi agent; Stephen Kuusisto. Pimedate planeet; Joanna Trollope. Mehed ja tütarlapsed; Detlef Jena. Venemaa tsaarinnad; Helle Helle. Maja ja kodu; Nick Hornby. Ühest poisist; Deepak Chopra. Eatu keha, ajatu vaim

the efficacy of xylopia aethiopica and piper guinense seeds powder

African Journals Online (AJOL)

Sammy

rice grain pests, it is essential that necessary control measures are put in place to ... Similarly, Teetes and Gilstrap (1986) were of the view that the use of chemical ... were killed, as suggested by Ivbijaro (1990) and Wee and Nick (1998).

A multiverse play divides opinion

Science.gov (United States)

Crease, Robert P.

2015-03-01

The stage lights rise. A man and woman meet in a cute way - "Do you know why it's impossible to lick the tips of your elbows?" she asks - they chat momentarily, and separate. The play is Constellations by Nick Payne.

Effects of Nordic Walking and Pilates training programs on aminotransferase activity in overweight and obese elderly women

OpenAIRE

Magdalena Hagner-Derengowska; Krystian Kałużny; Jacek Budzyński

2015-01-01

Hagner-Derengowska Magdalena, Kałużny Krystian, Budzyński Jacek. Effects of Nordic Walking and Pilates training programs on aminotransferase activity in overweight and obese elderly women. Journal of Education, Health and Sport. 2015;5(12):563-580. eISSN 2391-8306. DOIhttp://dx.doi.org/10.5281/zenodo.44249 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2812%29%3A563-580 http://pbn.nauka.gov.pl/works/687148 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X...

Effects of Nordic Walking and Pilates training programs on aminotransferase activity in overweight and obese elderly women

OpenAIRE

Hagner-Derengowska, Magdalena; Kałużny, Krystian; Budzyński, Jacek

2015-01-01

Hagner-Derengowska Magdalena, Kałużny Krystian, Budzyński Jacek. Effects of Nordic Walking and Pilates training programs on aminotransferase activity in overweight and obese elderly women. Journal of Education, Health and Sport. 2015;5(12):563-580. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.44248 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2812%29%3A563-580 http://pbn.nauka.gov.pl/works/687148 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665...

Aproximación de un modelo didáctico para la creación de objetos virtuales de aprendizaje

OpenAIRE

Arango Vásquez, Sandra Isabel; Universidad de Medellín; Vásquez Lopera, Claudia Patricia; Universidad de Medellín; Salazar Ceballos, Alexander; Universidad del Magdalena; Álvarez-Miño, Lídice; Universidad del Magdalena

2013-01-01

Este artículo muestra los hallazgos iniciales del proyecto “Modelo didáctico para la creación de objetos virtuales de aprendizaje soportado en una red académica de alta velocidad”. La Universidad de Medellín y la Universidad del Magdalena presentan una aproximación a este modelo teniendo en cuenta la conceptualización de los enfoques pedagógicos, los modelos pedagógicos y los modelos didácticos. Esta investigación de corte cualitativo con enfoque hermenéutico obtuvo como resultados iniciales ...

Efecto de la manipulación del semen criopreservado de bovinos Bos Taurus sobre la integridad espermática

OpenAIRE

Norberto Villa-Duque; Claudia Marcela Amaya-Torres; Darwin García-Rojas; Natalia Nieto-Omeara; Natalia Terán-Acuña

2016-01-01

En el estudio se evaluó el efecto de descongelar y aplicar semen de bovinos Bos Taurus en 33 ganaderías del Magdalena Medio colombiano, y se estudió in vitro el efecto de la injuria encontrada sobre la integridad de las membranas espermáticas. La información en fincas se recopiló mediante formulario específico, mientras que el estudio in vitro se ejecutó en el laboratorio de Biotecnología Reproductiva Animal del Instituto Universitario de la Paz (Barrancabermeja, Santander). El estudio consis...

Reconstrucción paleoambiental y asociaciones de foraminiferos de la formación Tubará (plioceno temprano), sección Arroyo Piedras (norte de Colombia)

OpenAIRE

Molinares Blanco, Carlos Eduardo

2007-01-01

La Formación Tubará en el Arroyo Piedras (169 m) es dividida en tres segmentos, interpretados como producto de la sedimentación en ambientes marino marginales relacionados con la evolución del paleo-delta del río Magdalena durante el Plioceno Temprano: El segmento inferior, es interpretado como resultado de sedimentación siliciclástica en una llanura aluvial/deltaica; el segmento intermedio, como el resultado de sedimentación lagunar y/o en pantanos costeros y el superior, como la transición ...

Estudio morfométrico para discriminar según el sexo, cangrejos adultos de la subespecie Hypolobocera Bouvieri Bouvieri (Rathbun, 1898 (Decápoda: Pseudothelphusidae

Directory of Open Access Journals (Sweden)

Campos Martha R.

1986-12-01

Full Text Available Se presentan los resultados de un análisis estadístico con cangrejos adultos de la subespecie Hypolobocera bouvieri bouvieri (Rathbun, 1898 de la cuenca del río Negro, hoya del río Magdalena, en el Departamento de Cundinamarca, Colombia.  El estudio consideró 38 especímenes (10 hembras y 28 machos y para cada ejemplar se observaron 29 variables cuantitativas. Utilizando métodos estadísticos multivariados se encontraron 7 variables relevantes con carácter discriminatorio según el sexo.

Sociocultural factors influencing control and prevention of vector-borne diseases: The case of zika in Santa Marta (Colombia, 2015-2016.

Directory of Open Access Journals (Sweden)

Astrid Lorena Perafán Ledezma

2017-03-01

Full Text Available Durante el año 2016, el grupo de Investigación en Diversidad Humana (IDHUM de la Universidad del Magdalena inició un proceso de investigación sobre conocimientos, percepciones y prácticas en torno al Zika y su vector en la ciudad de Santa Marta. Dado lo relevante del tema para la salud pública, consideramos pertinente comunicar a través de este medio algunos de nuestros hallazgos antes de terminar la investigación y que esta se publique en su totalidad.

Lista comentada de las plantas vasculares del enclave seco interandino de la tatacoa (huila, colombia)

OpenAIRE

FIGUEROA-C., YISELA; GALEANO, GLORIA

2012-01-01

Se presenta el inventario florístico del enclave seco interandino de La Tatacoa (vallede la parte alta del río Magdalena, Huila, Colombia). Se encontraron 223 especies,distribuidas en 170 géneros y 60 familias. A nivel florístico, la familia Leguminosaees la más diversificada con 36 especies y 28 géneros, seguida por Poaceae (20/15),Euphorbiaceae (13/6), Asteraceae (10/10) y Cactaceae (8/7). El hábito de crecimientopredominante en términos de riqueza de especies fue el de las hierbas, seguido...

Effect of a ten-week Nordic Walking exercise program on serum electrolyte concentration and plasma acid-base balance in postmenopausal women with overweight or obesity

OpenAIRE

Kałużny, Krystian; Kałużna, Anna; Budzyński, Jacek; Hagner, Wojciech; Kochański, Bartosz; Żukow, Walery; Bronisz, Agata; Hagner‑Derengowska, Magdalena

2016-01-01

Kałużny Krystian, Kałużna Anna, Budzyński Jacek, Hagner Wojciech, Kochański Bartosz, Żukow Walery, Bronisz Agata, Hagner‑Derengowska Magdalena. Effect of a ten-week Nordic Walking exercise program on serum electrolyte concentration and plasma acid-base balance in postmenopausal women with overweight or obesity. Journal of Education, Health and Sport. 2016;6(12):353-364. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.201929 http://ojs.ukw.edu.pl/index.php/johs/article/view/4065 ...

PERCEPCIÓN LOCAL DEL ESTADO AMBIENTAL EN LA CUENCA BAJA DEL RÍO MANZANARES

OpenAIRE

Plata, Ángela María; Ibarra Vega, Danny

2016-01-01

Los problemas ambientales que afectan a las cuencas hidrográficas en Colombia tienen múltiples causas, una de ellas es la ausencia de una cultura ambiental en las comunidades que viven o frecuentan zonas aledañas a las corrientes hídricas. El principal objetivo de este trabajo era recopilar la percepción local sobre el estado ambiental de la cuenca baja del río Manzanares, ubicada en el departamento del Magdalena e incentivar a los pobladores a reflexionar sobre su papel frente a la solución ...

II. Introducción: el Occidente de Colombia y el problema del origen de Cacicazgos

OpenAIRE

Dever, Alejandro; Espinosa, Iván; Langebaek, Carl Henrik; Piazini, Emilio

2015-01-01

El Valle de Aburrá está localizado en la Cordillera Central de Colombia. Geográficamente hablando, se trata de un valle intermedio entre la cuenca del Río Cauca, ubicada al occidente, y la del Río del Magdalena, localizada al oriente (Figura 1). Desde que se iniciaran estudios arqueológicos en Antioquia, ésta región, y en general el occidente de Colombia, se definió como crucial para comprender el desarrollo de sociedades complejas. Reichel-Dolmatoff (1965: 135), por ejemplo, consideraba que ...

Condición somática de la almeja Polymesoda solida (Veneroidea: Corbiculidae durante el periodo lluvioso, enel Parque Natural Isla de Salamanca, Caribe colombiano

Directory of Open Access Journals (Sweden)

María Virginia De La Hoz Aristizábal

2010-03-01

Full Text Available Se evaluó la condición somática de la almeja Polymesoda solida en tres lagunas del Parque Isla de Salamanca (abril a noviembre de 2004, durante el periodo lluvioso. En 60 individuos por laguna, se analizaron mensualmente índices de condición, rendimiento, relación talla-peso y madurez gonadal en fresco. También se midió salinidad, temperatura, pH, oxígeno disuelto, transparencia y profundidad del agua. Los mayores índices de condición coincidieron con descensos en el nivel del río Magdalena, especialmente en Poza Verde y El Torno. Estos índices fueron más altos en tallas entre 27mm y 33mm, donde también se ubicó la talla media de madurez sexual. En Poza Verde, laguna con mayor influencia del río, se presentaron los mayores índices de condición, rendimiento, peso húmedo y frecuencia de madurez, revelando una mejor condición de los organismos. El índice de condición (peso cuerpo/ peso concha presentó mayor coeficiente de regresión múltiple con la salinidad, pH y transparencia, sugiriendo la importancia del régimen hidrológico en su regulación. Las diferencias espaciales en las variables evaluadas, resaltan la necesidad de tener en cuenta la heterogeneidad de este tipo de estuarios para la conservación y manejo adecuado de la pesquería de P. solida.Somatic condition of the clam Polymesoda solida (Veneroidea: Corbiculidae in the rainy season of the Salamanca Island Natural Park, Colombian Caribbean. The estuarine clam Polymesoda solida is the main fishery resource in the Salamanca Island Natural Park. I sampled the clam in three interconnected lagoons (Poza Verde, El Torno and Atascosa during the rainy period (April to November 2004. Sixty individuals were monthly collected by hand on each lagoon; 30 to analyze two condition indexes (relationship between body and shell weight and between soft dry and wet weight, yield (meat percentage and length-weight relationships. Maturity of female gonad smears was assessed on the

76 FR 73608 - Reliability Technical Conference, North American Electric Reliability Corporation, Public Service...

Science.gov (United States)

2011-11-29

... or municipal authority play in forming your bulk power system reliability plans? b. Do you support..., North American Electric Reliability Corporation (NERC) Nick Akins, CEO of American Electric Power (AEP..., EL11-62-000] Reliability Technical Conference, North American Electric Reliability Corporation, Public...

Wallace ja Gromit tegid Hollywoodis puhta töö / Andris Feldmanis

Index Scriptorium Estoniae

Feldmanis, Andris, 1982-

2006-01-01

Hollywoodi animafilmide Annie-auhindade jagamisel võitis 9 auhinda täispikk animafilm "Wallace ja Gromit : Libaküüliku needus" ("Wallace & Gromit : The Curse of the Were-Rabbit") : režissöörid Nick Park ja Steve Box : Suurbritannia 2005

Observaciones ecológicas en la Isla de Salamanca (Depto. del Magdalena, Colombia Observaciones ecológicas en la Isla de Salamanca (Depto. del Magdalena, Colombia

Directory of Open Access Journals (Sweden)

Schnetter Marie-Luise

1969-06-01

Full Text Available Von August 1965 bis Juni 1967 wurden auf der Isla de Salamanca ökologische Untersuchungen durchgeführt. Das genannte Gebiet liegt an der kolumbianischen Atlantikküste zwischen Barranquilla und Ciénaga und wird im Norden von der karibischen See und im Süden von der Ciénaga Grande de Santa Marta begrenzt. Die mittlere Jahrestemperatur beträgt 28° C. Die Trockenzeit dauert fünf Monate, die jährliche Niederschlagsmenge liegt wahrscheinlich bei 700 mm. Der Boden besteht überall aus Sand.  Weite Teile der Insel, vor allem im Westen, sind mit Mangrove bedeckt. Im Osten, wo die Untersuchungen durchgeführt wurden, breitet sich auf höher gelegenen Stellen ein 5-7 m hohes Gebüsch aus Dornsträuchern und Kakteen aus, In Gebieten, die sich wenig über Meeresniveau erheben, ist der Boden versalzen und entweder vegetationslos oder mit Halophyten, vor allem Batis maritima und Sesuvium portulacastrum, bedeckt. Die Bodenversalzung wird hauptsächlich durch bis an die Oberfläche aufsteigendes und dort verdunstendes salzhaltiges Grundwasser verursacht.  Während der Trockenzeit lagert sich auf den unbewachsenen Flächen eine Salzkruste ab, die in der Regenzeit durch Niederschläge ausgewaschen wird. Der Salzgehalt der mit Halophyten bestandenen Gebiete kann in der Trockenzeit über den der unbewachsenen Stellen ansteigen, was möglicherweise auf die Wasseraufnahme durch die Pflanzen und den dadurch verstärkten Wasserentzug zurückzuführen ist.  Soweit das Grundwasser dicht unter der Bodenoberfläche steht, ist ein das ganze Jahr über hoher Bodenwassergehalt vorhanden. Je mehr das Niveau ansteigt, desto geringer wird er, Am Ende der Trockenzeit kann die Wasserversorgung an mit Pflanzen bestandenen Stellen stärker beansprucht sein als an vegetationslosen. Jedoch hält in der Regenzeit der bewachsenen Boden die Niederschläge besser fest als der unbewachsene. Der Trockenbusch breitet sich nur an solchen Stellen aus, an denen das salzhaltige Grundwasser nicht bis an die Oberfläche steigt. Die oberen Bodenschichten sind dann salzarm, haben aber auch in der Regenzeit nur einen geringen Wassergehalt. An der untersuchten Stelle war der Boden in Grundwassernähe nicht mehr durchwurzelt.  Die meisten der im Trockenbusch wachsenden Arten werfen im Laufe der Trockenzeit ihre Blätter ab. Der Wassergehalt von Arten mit derben Blättern wie Prosopis juliflora und Pithecellobium spec. liegt etwas unter 70% und schwankt nur geringfügig. Pereskia colombiana, die sukkulente Blätter mit einem Wassergehalt von etwa 90% hat, verliert ihre Blätter schon am Ende der Regenzeit. Die Halophyten Batis und Sesuvium haben sukkulente, immergrüne Blätter. Bei beiden Arten ist der Wassergehalt der Blätter in der Regenzeit höher als in der Trockenzeit. Der Wassergehalt der derben immergrünen Blätter von Laguncularia racemosa, einer Mangrovenpflanze, zeigt keine von der Jahreszeit abhängigen Schwankungen.  Es wurde das Längenwachstum von Batis maritima, die in einer feuchten Zone wuchsen, mit dem von Pflanzen eines trockenen Standorts verglichen.  In der Trockenzeit war das Wachstum an beiden Stellen beeinträchtigt, jedoch an dem trockenen Standort stärker als an dem feuchten.  An einer Stelle, an der zwei Jahre zuvor Ableger von Lemaireocereus griseus gepflanzt worden waren, die inzwischen untersehiedlich gut wuchsen, wurde der Salzgehalt des Bodens bestimmt. Er war an Orten, an denen die Kakteen besonders gut gediehen waren, nicht eindeutig niedriger als an anderen Stellen, an denen das Wachstum schlechter gewesen war.  Neben dem Salzgehalt spielt wahrscheinlich die Bodenstruktur eine wichtige Rolle für die Vegetationsentwicklung in diesem Gebiet. Desde agosto de 1965 hasta junio de 1967 se realizaron estudios ecológicos en la Isla de Salamanca. La región mencionada esta situada en la Costa Atlántica de Colombia, entre las ciudades de Ciénaga y Barranquilla, y es limitada por el Mar Caribe en el norte y la Ciénaga Grande de Santa Marta en el sur. La temperatura promedia anual es de 28° C. El verano dura cinco meses, la cantidad anual de lluvias sube probablemente a los 70 mm. En toda la región los suelos son arenosos. Vastas zonas de la Isla, sobre todo en el oeste, están cubiertas con manglares. En el este, donde se realizaron las investigaciones, las partes más elevadas están cubiertas con monte espinoso, mientras que en terrenos bajos (poco encima del nivel del mar hay tierras salinas desnudas o con vegetación de plantas halófilas, como Batis maritima y Sesuvium portulacastrum. Generalmente la salinidad del suelo es causada por aguas salinas subterráneas. Durante el verano la superficie de la tierra en las zonas desnudas esta cubierta con una costra de sal, mientras que en el verano los contenidos de sal en los estratos superiores son muy bajos. Las concentraciones de sal en áreas cubiertas con plantas halófilas pueden elevarse sobre las de las zonas desnudas, causada probablemente por la absorción de agua por las plantas. Donde el nivel de las aguas subterráneas esta cerca de la superficie de la tierra, hay un contenido alto de agua en todo el año, Al elevarse el nivel de la tierra sobre el nivel del mar, el disminuye. Al fin del verano el suministro de agua en las áreas cubiertas con plantas puede ser peor que en las zonas desnudas, pero en el invierno la cubierta vegetal reduce el desagüe superficial de las aguas de las lluvias.  Donde las aguas subterráneas no llegan hasta la superficie de la tierra, se puede desarrollar el monte espinoso, porque la concentración de sal baja notablemente en los estratos superiores. En el sitio investigado no se encontraron raíces cerca del nivel de las aguas subterráneas.  La mayoría de las especies creciendo en el monte espinoso pierde sus hojas durante el verano. Las especies con hojas fuertes, como Prosopis juliflora y Pithecollobium. spec, tienen un contenido de agua de 70%, aproximadamente. Las hojas suculentas de Pereskia colombiana, con un contenido de agua de 90%, desaparecen ya al fin del invierno. Las plantas halófilas, Batis y Sesuvium, tienen hojas suculentas siempre verdes. El contenido de agua de ambas especies es en el invierno superior al del verano.  El contenido de agua de las hojas de Laguncularia racemosa no muestra fluctuaciones dependientes de las estaciones.  Se compare el crecimiento de Batis maritima, creciendo en un lugar siempre húmedo, con el de plantas de una zona seca. Durante el verano el crecimiento disminuyo en ambos lugares, pero el perjuicio era peor en la zona seca que en la zona húmeda.  Se determinó el contenido de sal del suelo en un lugar donde había una plantación de Lemaireocereus griseus, sembrada dos años antes. No se pudieron encontrar diferencias considerables en el contenido de sal de lugares con plantas bien desarrolladas y otros con cactáceas poco crecidas.  Aparentemente, además del contenido de sal, la estructura de los suelos tiene alguna importancia para el desarrollo de la vegetación en aquellaregión.

Plasmid DNA damage caused by stibine and trimethylstibine

International Nuclear Information System (INIS)

Andrewes, Paul; Kitchin, Kirk T.; Wallace, Kathleen

2004-01-01

Antimony is classified as 'possibly carcinogenic to humans' and there is also sufficient evidence for antimony carcinogenicity in experimental animals. Stibine is a volatile inorganic antimony compound to which humans can be exposed in occupational settings (e.g., lead-acid battery charging). Because it is highly toxic, stibine is considered a significant health risk; however, its genotoxicity has received little attention. For the work reported here, stibine was generated by sodium borohydride reduction of potassium antimony tartrate. Trimethylstibine is a volatile organometallic antimony compound found commonly in landfill and sewage fermentation gases at concentrations ranging between 0.1 and 100 μg/m 3 . Trimethylstibine is generally considered to pose little environmental or health risk. In the work reported here, trimethylstibine was generated by reduction of trimethylantimony dichloride using either sodium borohydride or the thiol compounds, dithioerythritol (DTE), L-cysteine, and glutathione. Here we report the evaluation of the in vitro genotoxicities of five antimony compounds--potassium antimony tartrate, stibine, potassium hexahydroxyantimonate, trimethylantimony dichloride, and trimethylstibine--using a plasmid DNA-nicking assay. Of these five antimony compounds, only stibine and trimethylstibine were genotoxic (significant nicking to pBR 322 plasmid DNA). We found stibine and trimethylstibine to be about equipotent with trimethylarsine using this plasmid DNA-nicking assay. Reaction of trimethylantimony dichloride with either glutathione or L-cysteine to produce DNA-damaging trimethylstibine was observed with a trimethylantimony dichloride concentration as low as 50 μM and L-cysteine or glutathione concentrations as low as 500 and 200 μM, respectively, for a 24 h incubation

Turism rikastab : rahvusvaheline turismikonverents

Index Scriptorium Estoniae

2006-01-01

28. septembril toimus Tallinnas Rahvusraamatukogus rahvusvaheline turismikonverents"Turism rikastab". Ülevaade majandus- ja kommunikatsiooniministeeriumi majandusarengu asekantsleri Maria Värtoni, Graham Mckenzie, Linnar Viigi, Nick Skelloni, Mario Ascencao (Haagi ülikool), David Carsoni (Ulsteri ülikool), Vesa Heikkineni ja Imre Sooääre ettekannetest

Moskvas hoitav Adolf Hitleri kolbatükk ei kuulu füürerile / Aadu Hiietamm

Index Scriptorium Estoniae

Hiietamm, Aadu, 1954-

2009-01-01

USA arheoloogi Nick Bellantoni avastus, et Moskvas hoitav Adolf Hitleri kuuliauguga koljutükk ei kuulugi füürerile, annab tõenäoliselt uut hoogu vandenõuteoreetikutele, kes usuvad, et Hitler ei teinud 1945. aastal enesetappu, vaid põgenes välismaale

DNA expressions - A formal notation for DNA

NARCIS (Netherlands)

Vliet, Rudy van

2015-01-01

We describe a formal notation for DNA molecules that may contain nicks and gaps. The resulting DNA expressions denote formal DNA molecules. Different DNA expressions may denote the same molecule. Such DNA expressions are called equivalent. We examine which DNA expressions are minimal, which

Recent Naval Postgraduate School Publications

Science.gov (United States)

1988-09-30

pay, performance ratings and voluntary separations 12th Annual Convention, Eastern Econ. Asso., Philadelphia, PA, Apr., 1986. Sol nick, L M...ilow above the Marine boundary layer 4th Conference Meteorol. and Oceano . Coastal Zone, Anaheim, CA., February, 3-5, 1988. Smith, R Computational

Employment Relations in Denmark

DEFF Research Database (Denmark)

Madsen, Jørgen Steen; Due, Jesper Jørgen; Andersen, Søren Kaj

2011-01-01

Jørgen Steen Madsen, Jesper Due og Søren Kaj Andersen har skrevet et kapitel om udviklingen i dansk arbejdsmarkedsregulering til bogen International and Comparative Employment Relations, redigeret af Greg Bamber, Russell Lansbury og Nick Wailes. Bogen indeholder bidrag, der præsenterer og...

The Guatemalan Military: Transition from War to Peace

Science.gov (United States)

2001-06-01

1992. Cerdas Cruz, Rodolfo. El Precio de Una Herencia. Fuerza Armadas, Democracia y Derechos Humanos en Centroamérica. San José: Instituto...Interamericano de Derechos Humanos , 1996. Cullather, Nick. Secret History, The CIA’s Classified Account of Its Operations in Guatemala, 1952-1954. Stanford

Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment

Directory of Open Access Journals (Sweden)

Banasiuk R

2016-01-01

Full Text Available Rafał Banasiuk,1,* Joanna E Frackowiak,2,* Marta Krychowiak,1 Marta Matuszewska,1 Anna Kawiak,1 Magdalena Ziabka,3 Zofia Lendzion-Bielun,4 Magdalena Narajczyk,5 Aleksandra Krolicka1 1Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 2Department of Pathophysiology, Medical University of Gdansk, Gdansk, 3Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, AGH-University of Science and Technology, Kraków, 4Institute of Chemical and Environment Engineering, West Pomeranian University of Technology, Szczecin, 5Faculty of Biology, Laboratory of Electron Microscopy, University of Gdansk, Gdansk, Poland *These authors contributed equally to this work Abstract: A fast, economical, and reproducible method for nanoparticle synthesis has been developed in our laboratory. The reaction is performed in an aqueous environment and utilizes light emitted by commercially available 1 W light-emitting diodes (λ =420 nm as the catalyst. This method does not require nanoparticle seeds or toxic chemicals. The irradiation process is carried out for a period of up to 10 minutes, significantly reducing the time required for synthesis as well as environmental impact. By modulating various reaction parameters silver nanoparticles were obtained, which were predominantly either spherical or cubic. The produced nanoparticles demonstrated strong antimicrobial activity toward the examined bacterial strains. Additionally, testing the effect of silver nanoparticles on the human keratinocyte cell line and human peripheral blood mononuclear cells revealed that their cytotoxicity may be limited by modulating the employed concentrations of nanoparticles. Keywords: antimicrobial activity, green synthesis, nanocubes, nanospheres 

Innovative enclosure dome/observing aperture system design for the MROI Array Telescopes

Science.gov (United States)

Busatta, A.; Marchiori, G.; Mian, S.; Payne, I.; Pozzobon, M.

2010-07-01

The close-pack array of the MROI necessitated an original design for the Unit Telescope Enclosure (UTE) at Magdalena Ridge Observatory. The Magdalena Ridge Observatory Interferometer (MROI) is a project which comprises an array of up to ten (10) 1.4m diameter mirror telescopes arranged in a "Y" configuration. Each of these telescopes will be housed inside a Unit Telescope Enclosure (UTE) which are relocatable onto any of 28 stations. The most compact configuration includes all ten telescopes, several of which are at a relative distance of less than 8m center to center from each other. Since the minimum angle of the field of regard is 30° with respect to the horizon, it is difficult to prevent optical blockage caused by adjacent UTEs in this compact array. This paper presents the design constraints inherent in meeting the requirement for the close-pack array. An innovative design enclosure was created which incorporates an unique dome/observing aperture system. The description of this system focuses on how the field of regard requirement led to an unique and highly innovative concept that had to be able to operate in the harsh environmental conditions encountered at an altitude of 10,460ft (3,188m). Finally, we describe the wide use of composites materials and structures (e.g. glass/carbon fibres, sandwich panels etc.) on the aperture system which represents the only way to guarantee adequate thermal and environmental protection, compactness, structural stability and limited power consumption due to reduced mass.

Towards a systemic assessment of environmental impact (SAEI regarding alternative hydrosedimentological management practice in the Canal del Dique, Colombia

Directory of Open Access Journals (Sweden)

Leonel Vega Mora

2013-09-01

Full Text Available The Canal del Dique, located on the Colombian Caribbean Coast, can be seen as an "artificial" branch of the River Magdalena. Since its inception in 1650, it has undergone a series of interventions aimed at optimising river navigation between Cartagena and the interior of the country, little consideration having been paid to institutional, economic, social and environmental impacts.This paper presents a systemic assessment of environmental impact (SAEI methodology, based on systemic parameterisation of the environment, proposed by Vega [2011], for the strategic environmental assessment of policy, plans, programmes or large-scale projects, considering a case study of the Canal del Dique environmental rehabilitation as proposed by the Colombian government.Information arising from the framework of an inter-administrative agreement between the Universidad Nacional de Colombia and the Regional Autonomous Corporation for the Rio Grande de la Magdalena (CORMAGDALENA was used for studying the environmental restoration of and navigation in the Canal del Dique [UN-LEH, 2008]. The authors developed and summarised SAEI regarding six alternatives for hydrosedimentological management in the canal to obtain results concerning environmental impairment or improvement regarding each factor considered, and the degree of aggressiveness or betterment involved in each alternative.Alternative 4, an enhanced version of current condition (ECC would have produced the greatest environmental benefit (i.e. limited dredging plus optimising channel-wetland interconnection requiring the narrowing of three straight reaches along the canal and the construction of a sluice-gate system in the Strait of Paracuica (near the town of El Recreo.

Características de las instalaciones adaptadas

Directory of Open Access Journals (Sweden)

Editorial, Equipo

1968-11-01

Full Text Available A number of secondary adaptations were carried out at some of the Olympic installations. These included the improvement of the spectator seats, zones for the information services, increase in the number of wash rooms, so that the Olympic formal requirements could be met at the following specific Olympic sites: The Mexico Arena, for the boxing competitions; the Avandaro & Campo Marte, for the riding competitions; the Azteca Stadium, for football; the Auditorio Nacional, for gymnastics; the Estadio Municipal y Secundario de la «Magdalena Mixhuca », for hockey; the Teatro de los Insurgentes, for weightlif ting ; the Pista Hielo Insurgentes, for wrestling; Club de Yates at Acapulco, for sailing; Pista Hielo Revolución, for volleyball; and the Piscina de la Ciudad Universitaria, for water polo.Se hicieron una serie de adaptaciones secundarias: mejora de los asientos para espectadores, creación de zonas para los servicios de información, ampliación del número de cancelas y aseos, etcétera, de modo que satisficiesen los requisitos olímpicos reglamentarios en las siguientes instalaciones ya existentes: El Arena México, para la celebración de los combates de boxeo; el Avándaro y Campo Marte, para las pruebas ecuestres; Estadio Azteca, fútbol; Auditorio Nacional, gimnasia; Estadio Municipal y Secundarios de la «Magdalena Mixhuca», hockey; Teatro de los Insurgentes, levantamiento de pesas; Pista Hielo Insurgentes, lucha; Club de Yates en Acapulco, vela; Pista Hielo Revolución, balonvolea, y Piscina de la Ciudad Universitaria, water-polo.

Ictiotoxismo por consumo de barracuda (Sphyraena barracuda y morena manchada (Gymnothorax moringa en la comunidad de pescadores artesanales de Tasajera, caribe colombiano

Directory of Open Access Journals (Sweden)

Juan Diego Gaitán Espitia

2013-10-01

Full Text Available ResumenSe estudió un evento de ictiotoxismo en la comunidad de pescadores de la población de Tasajera, departamento del Magdalena. La intoxicación por ciguatera fue diagnosticada a 7 individuos cuyas edades oscilaban entre los 17 y 53 años (63,4% de rango de edades de los pescadores del sector, con síntomas de vómito (100%, dolor muscular en miembros inferiores (71,4%, espasmos abdominales (85,7%, diarrea (100%, entumecimiento y hormigueo de cara, manos, pies (85,7%, mareo (100%, y brote cutáneo (14,2%, los síntomas en la mayoría de los casos desaparecieron en un lapso de 8 a 12 días. Se determinó como causa principal del evento de ciguatera el consumo de carne de barracuda (Sphyraena barracuda y morena (Gymnothorax moringa. (Duazary 2007; 2: 160 - 167AbstractAn icthyotoxism event was studied in the Tasajera fishermen’s community, department of Magdalena. The ciguatera intoxication was diagnosed to 7 individuals whose ages were ranging between 17 and 53 years (63,4 %, with symptoms of vomit (100 %, muscular pain in low members (71,4 %, abdominal spasms (85,7 %, diarrhea (100 %, numbness and tingling of face, hands, feet (85,7 %, sickness (100 %, and cutaneous outbreak (14,2 %, the symptoms in the majority of the cases disappeared in a space from 8 to 12 days. The consumption of the barracuda (Sphyraena barracuda spotted moray (Gymnothorax moringa meat was determined as principal source of the ciguatera event.Key words: Icthyotoxism; Ciguatera; Intoxication; Colombian Caribbean.

The Myth of Religious Experience Revisited

Directory of Open Access Journals (Sweden)

Zangwill Nick

2017-10-01

Full Text Available This article replies to Manuel Fasko’s “The Demystification of Nick Zangwill’s ‘Myth of Religious Experience’” (2017, showing how author’s argument against the possibility of religious experience presented in “The Myth of Religious Experience” (2004 remains in tact.

Limited numbers of apoptotic cells in fresh paraffin embedded bone marrow samples of patients with myelodysplastic syndrome

NARCIS (Netherlands)

Brada, SJL; van de Loosdrecht, AA; Koudstaal, J; de Wolf, JTM; Vellenga, E

In myelodysplasia (MDS) the precise mechanism of ineffective erythropoiesis is not fully elucidated, but it is suggested that apoptosis may contribute to this process. We performed TdT-mediated dUTP-nick end labelling (TUNEL) staining of paraffin embedded bone marrow specimens to assess the amount

76 FR 36903 - Privacy Act of 1974; System of Records

Science.gov (United States)

2011-06-23

... have a need-to-know. Individuals responsible for servicing the records in the performance of official... name, nick names, Social Security Number (SSN), gender, date of birth, personal cell phone number, home... address: Delete entry and replace with ``Automated Civil Engineer System/ Interim Work Management System...

Berlinale terad / Kadri Kõusaar

Index Scriptorium Estoniae

Kõusaar, Kadri

2006-01-01

56. Berliini festivali filme - Jasmila Zhbanici "Grbavica", Michael Winterbottomi, Mat Whitecrossi "Teekond Guantanamosse", tailase Pen-ek Ratanaruangi "Nähtamatud lained", John Hillcoati "Ettepanek" (stsenarist ja muusika autor Nick Cave), Bennett Milleri "Capote" (nimiosas P.S. Hoffman), Yang Yong-hi dokfilm "Kallis Pyongyang", Tomer Heymanni dokfilm "Pabernukud"

The good, the bad, and the voter: the impact of hate speech prosecution of a politician on the electoral support for his party

NARCIS (Netherlands)

van Spanje, J.; de Vreese, C.

2015-01-01

Hate speech prosecution of politicians is a common phenomenon in established democracies. Examples of politicians tried for hate speech include Nick Griffin in Britain and Jean-Marie Le Pen in France. Does hate speech prosecution of politicians affect the electoral support for their party? This is

Zarqawi’s Sfumato: Operational Art in Irregular Warfare

Science.gov (United States)

2013-05-23

in Baghdad and Karbala during the Shia festival of Ashura in March of 2004.80 These attacks were preceded by the bombing of the Jordanian embassy...American contractors Nick Berg and Eugene Armstrong. Zarqawi released edited videos with music and logos detailing multiple suicide bombings against

Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA

International Nuclear Information System (INIS)

Nakabeppu, Y.; Sekiguchi, M.

1981-01-01

T4 endonuclease, which is involved in repair of uv-damaged DNA, has been purified to apparent physical homogeneity. Incubation of uv-irradiated poly(dA).poly(dT) with the purified enzyme preparations resulted in production of alkali-labile apyrimidinic sites, followed by formation of nicks in the polymer. By performing a limited reaction with T4 endonuclease V at pH 8.5, irradiated polymer was converted to an intermediate form that carried a large number of alkali-labile sites but only a few nicks. The intermediate was used as substrate for the assay of apurinic/apyrimidinic DNA endonuclease activity. The two activities, a pyrimidine dimer DNA glycosylase and an apurinic/apyrimidinic DNA endonuclease, were copurified and found in enzyme preparations that contained only a 16,000-dalton polypeptide. These results strongly suggested that a DNA glycosylase specific for pyrimidine dimers and an apurinic/apyrimidinic DNA endonuclease reside in a single polypeptide chain coded by the denV gene of bacteriophage T4

?Strange Attractors (chaos) in the hydro-climatology of Colombia?

International Nuclear Information System (INIS)

Poveda Jaramillo, German

1997-01-01

Inter annual hydro-climatology of Colombia is strongly influenced by extreme phases of ENSO, a phenomenon exhibiting many features of chaotic non-linear system. The possible chaotic nature of Colombian hydrology is examined by using time series of monthly precipitation at Bogota (1866-1992) and Medellin (1908-1995), and average stream flows of the Magdalena River at Puerto Berrio. The power spectrum, the Haussdorf-Besikovich (fractal) dimension, the correlation dimension, and the largest Lyapunov exponent are estimated for the time series. Ideas of hydrologic forecasting and predictability are discussed in the context of nonlinear dynamical systems exhibit chaotic behavior

Construcción y deconstrucción territorial del Caribe Colombiano durante el siglo XIX

OpenAIRE

Domínguez, Camilo; Chaparro Mendivelso, Jeffer; Gómez, Carla

2007-01-01

El proceso de estructuración territorial del Caribe Granadino durante el siglo XIX, específicamente de 1810 a 1886, se aborda desde una perspectiva geohistórica y geopolítica. Se parte de la base ambiental de la región, para luego abordar la evolución de los límites administrativos mediante reconstrucciones cartográficas basadas en mapas históricos. El proceso de cambio abarcó los antiguos estados de Magdalena y Bolívar (incluyendo las islas de San Andrés y Providencia), la costa caribe de Pa...

Geografía médica, bacteriología y el caso de las fiebres en Colombia en el siglo XIX

Directory of Open Access Journals (Sweden)

Mónica García

2012-04-01

Full Text Available This article explores the relation between bacteriology and medical geography in the 19th century in Colombia, following the case of fevers. Through this, it explains how historiography has approached the relation between bacteriology and medical geography, and analyzes said relation in the medical research pertaining to yellow fever, fevers of the Magdalena River, and malaria in the 1880s. Finally, it shows that, instead of passively incorporating bacteriology, the physicians fought to combine it with medical geography, incorporating elements of both approaches, keeping them in tense separation, or rejecting one in favor of the other.

Cerambycidae (Coleoptera da Colômbia: IV. Cerambycinae com olhos grosseiramente granulados Cerambycidae (Coleoptera from Colômbia: IV. Cerambycinae with coarsely granulated eyes

Directory of Open Access Journals (Sweden)

Ubirajara R. Martins

2003-01-01

Full Text Available New records for Colombian Cerambycidae - Achrysonini: Achryson immaculipenne Gounelle, 1909, A. lineolatum Erichson, 1847, A. quadrimaculatum (Fabricius, 1792; Ectenessini: Ectenessa lurida Martins, 1973; Cerambycini: Jupoata rufipennnis (Gory, 1831, Poeciloxestia carlyslei Fragoso, 1978, P. paraensis Lane, 1965; Elaphidionini: Ambonus electus (Gahan & Arrow, 1903, Paramallocera cribripennis (Bates, 1885, Periboeum pubescens (Olivier, 1790, Stizocera plicicollis (Germar, 1824, S. poeyi (Guérin-Méneville, 1838, Pantonyssus glabricollis Fuchs, 1961; Piezocerini: Haruspex lineolatus Bates, 1870; Eligmodermini: Eligmoderma spinicolle Aurivillius, 1923, E. ziczac Nonfried, 1895. New species described: Pantonyssus suturale sp. nov. (Santander; Anelaphus colombianus sp. nov. (Santander; A. sparsus sp. nov. (Magdalena.

COMPETENCIA MATEMÁTICA EN NIÑOS EN EDAD PREESCOLAR - MATH COMPETENCY IN PRE-SCHOOL AGE CHILDREN

Directory of Open Access Journals (Sweden)

MYRIAM ESTHER ORTIZ PADILLA

2009-06-01

Full Text Available This study identifies the characteristics of Mathematical competency in pre-school age children in the Magdalena region. The population was represented by 101 children, to whom the Basic Mathematics Competency Test, Item 3, in its Spanish version, was administered. Quantitative methodology was used, from an empirical and analytical approach and a cross-sectional design was implemented. The results indicate that 31% of children evaluated obtaineda Mathematics Competency Global Index average, with 57% for descriptors: below averageand 22% above average. The private institutions placed a higher percentage of students aboveaverage. The sex and age variable does not provide significant differences.

What is Soft Computing? Bridging Gaps for 21st Century Science!

Directory of Open Access Journals (Sweden)

Rudolf Seising

2010-06-01

Full Text Available This contribution serves historical and philosophical reflecting cognitions on the role of Soft Computing in the 21st century. Referring to Magdalena's article in this issue, this paper considers the aspects of mixtures of techniques, the opposite pair qHard Computingq and qSoft Computingq, and Computational Intelligence. From the historical perspective the paper goes back to three articles by Warren Weaver that appeared after World War II. A concentrated study of these papers helps to understand that Soft Computing will be able to play a key role in the future development of science and technology.

Parish, bills and some economic abuses

OpenAIRE

Citterio, Diego Ezequiel

2008-01-01

El estudio de la Iglesia colonial de la campaña de Buenos Aires ha sido abordado desde distintas perspectivas y aristas; desde el análisis de la recolección del diezmo hasta el funcionamiento de una estancia eclesiástica, desde el culto a la virgen de Luján hasta la conformación de cofradías. En este trabajo el tema es la parroquia de Magdalena y sus estrategias de subsistencia económicas en la zona de frontera. Intentado establecer a partir del análisis de los libros de fábrica algunas consi...

Depresión y factores socio demográficos asociados en estudiantes universitarios de ciencias de la salud de una universidad publica (Colombia)*

OpenAIRE

Ferrel Ortega, Robert F.; Celis Barros, Adela; Hernández Cantero, Olga

2011-01-01

Se trata de una investigación descriptiva, con diseño transversal, realizada con el objetivo identificar los niveles de depresión y factores sociodemográficos asociados, en 190 estudiantes de Psicología, Medicina, Odontología y Enfermería, de una Universidad Pública del Magdalena (35,8% hombres y 64,2% mujeres, media de 20,4 años y D.T. de 2,7). Se aplicó el "Inventario de Depresión de Beck" (BDI, Beck, Rush, Shaw y Emery, 1979) y se registraron datos sociodemográficos. Se halló que el 52% su...

El Laboratorio Clínico y el Dengue

OpenAIRE

Gilberto Angel M.

1990-01-01

El dengue es una enfermedad endémica de zonas tropicales y subtropicales, en regiones que se encuentran por debajo de los 1.800 metros sobre el nivel del mar.

En 1969 se registraron varios brotes en el Caribe, Puerto Rico e Islas Vírgenes y en 1970 se diagnosticó en Barranquilla. En !975 se encontraron en el Magdalena medio unos 450 casos. En 1979 se señalaron unos 3.000 casos en México y en 1981 se padeció en Cuba una gran epidemia de dengue clásico tipo 1, que afe...

Revival of Palagua

International Nuclear Information System (INIS)

Carta Petrolera

1995-01-01

In Palagua, a extinct oil concession, of the Magdalena Medio (Colombia) the environmental aspects, changes day by day to the compass of the technical and human effort of ECOPETROL; natural pools that accumulated oily during decades, mud and other residuals of the oil exploitation and they caused focuses of contamination, today in day they are in extinction way. The residual waters, produced jointly with the petroleum, are reinjected to the underground after going by a complex purification process. The solids residuals, receives treatment adapted in built sanitary filler with modern techniques for the gases escape and liquids produced by the materials decomposition

Corredor Bimodal Cafetero

OpenAIRE

Duque Escobar, Gonzalo

2015-01-01

El Corredor Bimodal Cafetero es un proyecto de infraestructura estratégica que articula la Hidrovía del Magdalena con el Corredor Férreo del río Cauca, inscrito en el Plan Nacional de Desarrollo 2014/2018 y financiable con la salida de 30 mil toneladas diarias de carbón andino a la cuenca del Pacífico. Incluye el Túnel Cumanday para cruzar la Cordillera Central, el Ferrocarril Cafetero de 150 km y 3% de pendiente entre La Dorada y el Km 41, y la Transversal Cafetera de 108 km para una vía de...

Children in Hot Cars Result in Fatal Consequences

Medline Plus

Full Text Available ... a hot vehicle. “Putting it bluntly, leaving your child in a hot car is like leaving your child in a lit oven,” said Dr. Nick Jouriles. “ ... or experience changes in your schedule or your child is in the back seat, making it more ...

The Significance of Bottom in Shakespeare's A Midsummer Night's Dream

OpenAIRE

KREMENIK, Michael

1999-01-01

The importance of the character Nick Bottom in A Midsummer Night's Dream will be discussed. He is the only character able to converse with both the natural and fairy worlds in the play. This makes his character special. This article will attempt to analyze why this is so.

76 FR 56196 - Agency Information Collection Activities; Proposed Collection; Comment Request

Science.gov (United States)

2011-09-12

..., and IV); (b) the FTC's consumer complaint systems; (c) the FTC's program evaluation activities and (d... (Nick M) and Ami Dziekan (Ami D), Division of Enforcement, Bureau of Consumer Protection, Federal Trade... Commission's Rules of Practice (primarily Parts I, II, and IV); (b) the FTC's consumer complaint systems; (c...

Fighting Fire with Fire

DEFF Research Database (Denmark)

Bossetta, Michael

2017-01-01

Advancing the concept of populism as a political style, this study compares the debate performances of two British party leaders, Nick Clegg and Nigel Farage, as they clashed in a pair of televised debates over Britain’s EU membership ahead of the 2014 European Parliament elections. The argument ...

Uudised

Index Scriptorium Estoniae

2005-01-01

Eesti artisti Pafi ehk Paavo Eensalu lugu "Daisy" ilmub septembris Nick Luscombe poolt kokkupandaval kogumikul "Flo-Motion vol 2". Popansambel Radiohead alustas oma uut LPd tehes blogi ehk internetipäeviku "Dead Air Space" pidamist, aadressilt http://www.radiohead.com/deadairspace/ võib leida bändiliikmete kirjutisi

Ostrich: Journal of African Ornithology - Vol 88, No 1 (2017)

African Journals Online (AJOL)

DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Abdulmaula Hamza, Nicola Baccetti, Joe Sultana, Jaber Yahia, Marco Zantello, Adriano de Faveri, Nick Cutts, John Borg, Hichem Azafzaf, Pierre Defos du Rau, Esam Bourass, Khaled Etayeb, Michael Elliott, 53–58. http://dx.doi.org/10.2989/00306525.2016.1231139 ...

77 FR 8892 - Detailed Planning To Consider Additional Land Protection on the Missouri River From Fort Randall...

Science.gov (United States)

2012-02-15

... 25486, DFC, Denver, CO 80225. FOR FURTHER INFORMATION CONTACT: Nick Kaczor, Planning Team Leader... old, wider river valley contains important habitat for at least 60 native and 26 sport fish. In... historical characteristics witnessed by Lewis and Clark during their explorations in the early 1800s. The FWS...

Muusika DVD-d / Priit Hõbemägi

Index Scriptorium Estoniae

Hõbemägi, Priit, 1957-

2006-01-01

Uutest DVD-dest Moody Blues "Lovely to See You - Live", Nick Cave and The Bad Seeds "Road to God Knows Wherw", Thin Lizzy "Thunder and Lightning Tour", Eels "Eels With Strings - Live at Town Hall", Moby "Live. Hotel Tour 2005", Corvus Corax "Cantus Buranus", Destiny's Child "Live in Atlanta"

Characterization of the adenoassociated virus Rep protein complex formed on the viral origin of DNA replication

International Nuclear Information System (INIS)

Li Zengi; Brister, J. Rodney; Im, Dong-Soo; Muzyczka, Nicholas

2003-01-01

Interaction between the adenoassociated virus (AAV) replication proteins, Rep68 and 78, and the viral terminal repeats (TRs) is mediated by a DNA sequence termed the Rep-binding element (RBE). This element is necessary for Rep-mediated unwinding of duplex DNA substrates, directs Rep catalyzed cleavage of the AAV origin of DNA replication, and is required for viral transcription and proviral integration. Six discrete Rep complexes with the AAV TR substrates have been observed in vitro, and cross-linking studies suggest these complexes contain one to six molecules of Rep. However, the functional relationship between Rep oligomerization and biochemical activity is unclear. Here we have characterized Rep complexes that form on the AAV TR. Both Rep68 and Rep78 appear to form the same six complexes with the AAV TR, and ATP seems to stimulate formation of specific, higher order complexes. When the sizes of these Rep complexes were estimated on native polyacrylamide gels, the four slower migrating complexes were larger than predicted by an amount equivalent to one or two TRs. To resolve this discrepancy, the molar ratio of protein and DNA was calculated for the three largest complexes. Data from these experiments indicated that the larger complexes included multiple TRs in addition to multiple Rep molecules and that the Rep-to-TR ratio was approximately 2. The two largest complexes were also associated with increased Rep-mediated, origin cleavage activity. Finally, we characterized a second, Rep-mediated cleavage event that occurs adjacent to the normal nicking site, but on the opposite strand. This second site nicking event effectively results in double-stranded DNA cleavage at the normal nicking site

DNA replication: stalling a fork for imprinting and switching

DEFF Research Database (Denmark)

Egel, Richard

2004-01-01

Mating-type switching in fission yeast has long been known to be directed by a DNA 'imprint'. This imprint has now been firmly characterized as a protected site-specific and strand-specific nick. New work also links the widely conserved Swi1-Swi3 complex to the protection of stalled replication...

Beyond Tokenism: Teaching a Diverse History in the Post-14 Curriculum

Science.gov (United States)

Dennis, Nick

2016-01-01

Nick Dennis shows how a "multidirectional memory" approach to teaching history can move history teachers beyond seeing black history as separate or distracting from the history that must be taught at examination level. He gives examples of ways in which a diverse history can be built into examination courses, strengthening historical…

CNS wound healing is severely depressed in metallothionein I- and II-deficient mice

DEFF Research Database (Denmark)

Penkowa, M; Carrasco, J; Giralt, M

1999-01-01

. In contrast to normal mice, at 20 dpl no wound healing had occurred. The rate of apoptosis, as determined by using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling, was drastically increased in neurons of ipsilateral cortex of the MT-I+II null mice. Our results demonstrate that MT...

Work-Life Balance: the Links with Management Practices and Productivity

OpenAIRE

Nick Bloom; Tobias Kretschmer; John Van Reenen

2006-01-01

Does good management and higher productivity come at the expense of work-life balance? Or is good work-life balance an important component of the management of successful firms? New research by Nick Bloom, Tobias Kretschmer and John Van Reenen finds evidence for a hybrid view between these two polar extremes.

Research Staff | Photovoltaic Research | NREL

Science.gov (United States)

Research Staff Research Staff desc Greg Wilson Center Director Dr. Greg Wilson is the Director of @nrel.gov 303-384-6649 Bosco, Nicholas Staff Scientist Nick.Bosco@nrel.gov 303-384-6337 Braunecker, Wade IV-Physics Michael.Deceglie@nrel.gov 303-384-6104 Deline, Chris Staff Engineer Chris.Deline@nrel.gov

Development Program for Field-Repairable/Expendable Main Rotor Blades

Science.gov (United States)

1976-09-01

curve, based on the data obtained from this testing. In this curve, the runout points are plotted with arrows and the fatigue curve is drawn through...paper, or abrasive wheel , blend out scratch, nick, pitting, etc. CAUTION The final polish of smooth blending will be in a spanwise direction. Remove

Ganoderic acid B's influence towards the therapeutic window of ...

African Journals Online (AJOL)

9. Hempel C, Norenberg W, Sobottka H, Urban N, Nicke. A, Fischer W, Schaefer M. The phenothiazine-class an- tipsychotic drugs prochlorperazine and trifluoperazine are potent allosteric modulators of the human P2X7 receptor. Neuropharmacology 2013; 75:365-379. 10. Song H, Tao Y, Zhang CZ, Zhou YD. Comparison.

Remember the Algae that Went to Space? Here's What Happened Next | News |

Science.gov (United States)

. He met with Dr. Alexandra Dubini, at the time a research scientist at NREL working with the algae education how to grow algae. Nick Sweeney, a research technician in NREL's National Bioenergy Center . vulgaris to shift into lipid production mode. Squares of a special tape that changes color from tan to gray

Muusika / Mart Juur

Index Scriptorium Estoniae

Juur, Mart, 1964-

2005-01-01

Uutest heliplaatidest Pia Fraus "Sailing On A Grapefruit Lake", Six Organs Of Admittance "School Of The Flower", Jaga Jazzist "What We Must", Oasis "Don't Belive The Truth", Limp Bizkit "The Unquestionable Truth", "Do Me Bad Things", Saari, Jarmo "Solu", Cave, Nick & The Bad Seeds "B-Sides & Rarities", "Stars Wars", Weezer "Make Believe"

Storage stability of flour-blasted brown rice

Science.gov (United States)

Brown rice was blasted with rice flour rather than sand in a sand blaster to make microscopic nicks and cuts so that water can easily penetrate into the brown rice endosperm and cook the rice in a shorter time. The flour-blasted American Basmati brown rice, long grain brown rice, and parboiled long...

Cognitive Psychology and Low-Stakes Testing without Guarantees

Science.gov (United States)

Dennis, Nick

2016-01-01

The emphasis on the power of secure substantive knowledge reflected in recent curriculum reforms has prompted considerable interest in strategies to help students retain and deploy such knowledge effectively. One strategy that has been strongly endorsed by some cognitive psychologists is regular testing; an idea that Nick Dennis set out to test…

Olvido y memoria en Santa Marta, Colombia: El punto ciego de la esclavitud

Directory of Open Access Journals (Sweden)

Véronique Benei

2014-01-01

Full Text Available En este capítulo se trata de memorias - y obliteraciones - de la esclavitud en la ciudad de Santa Marta, Magdalena, en Colombia. Desde la perspectiva de la antropología histórica, se investigan las maneras en qué la historia y la práctica de la esclavitud parecen poco “recordadas” en esa ciudad, a pesar de haber sido un comercio comprobado y efectuado hasta el siglo XIX. Asimismo, se explora la articulación de estas obliteraciones memoriales con la utilización cotidiana, hoy en día, del registro discursivo de la esclavitud. Esta utilización ocurre en contextos muy variados, desde lo doméstico a la esfera del trabajo, y más sobre todo en el campo económico. Para terminar, se proponen varias hipótesis en cuanto a esta resurgencia discursiva.Palabras Clave: Santa Marta; Esclavitud; Comercio de esclavos.Social ethics and the dignity of human life: epistemic reach in societyAbstractThis chapter is about memories - and obliterations – of slavery in the city of Santa Marta, Magdalena, Colombia. From the perspective of historical anthropology, it investigates the ways in which the history and practice of slavery seem less “remembered” in that city, although it was a proved trade, and carried out during the nineteenth century. It also explores the articulation of these memorial obliterations, concerning nowadays the discursive speech of slavery. This usage occurs in diverse circumstances, such as the domestic environment and the sphere of work, and mainly in the economic atmosphere. Finally, we propose several hypotheses regarding this resurgence discourse.Keywords: Slavery; Santa Marta; slave trade.

Upper and Middle Crustal Velocity Structure of the Colombian Andes From Ambient Noise Tomography: Investigating Subduction-Related Magmatism in the Overriding Plate

Science.gov (United States)

Poveda, Esteban; Julià, Jordi; Schimmel, Martin; Perez-Garcia, Nelson

2018-02-01

New maps of S velocity variation for the upper and middle crust making up the northwestern most corner of South America have been developed from cross correlation of ambient seismic noise at 52 broadband stations in the region. Over 1,300 empirical Green's functions, reconstructing the Rayleigh wave portion of the seismic wavefield, were obtained after time and frequency-domain normalization of the ambient noise recordings and stacking of 48 months of normalized data. Interstation phase and group velocity curves were then measured in the 6-38 s period range and tomographically inverted to produce maps of phase and group velocity variation in a 0.5° × 0.5° grid. Velocity-depth profiles were developed for each node after simultaneously inverting phase and group velocity curves and combined to produce 3-D maps of S velocity variation for the region. The S velocity models reveal a 7 km thick sedimentary cover in the Caribbean region, the Magdalena Valley, and the Cordillera Oriental, as well as crustal thicknesses in the Pacific and Caribbean region under 35 km, consistent with previous studies. They also display zones of slow velocity at 25-35 km depth under regions of both active and inactive volcanism, suggesting the presence of melts that carry the signature of segmented subduction into the overriding plate. A low-velocity zone in the same depth range is imaged under the Lower Magdalena Basin in the Caribbean region, which may represent either sublithospheric melts ponding at midcrustal levels after breaching through a fractured Caribbean flat slab or fluid migration through major faults within the Caribbean crust.

Jaguar Densities across Human-Dominated Landscapes in Colombia: The Contribution of Unprotected Areas to Long Term Conservation.

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Valeria Boron

Full Text Available Large carnivores such as jaguars (Panthera onca are species of conservation concern because they are suffering population declines and are keystone species in their ecosystems. Their large area requirements imply that unprotected and ever-increasing agricultural regions can be important habitats as they allow connectivity and dispersal among core protected areas. Yet information on jaguar densities across unprotected landscapes it is still scarce and crucially needed to assist management and range-wide conservation strategies. Our study provides the first jaguar density estimates of Colombia in agricultural regions which included cattle ranching, the main land use in the country, and oil palm cultivation, an increasing land use across the Neotropics. We used camera trapping across two agricultural landscapes located in the Magdalena River valley and in the Colombian llanos (47-53 stations respectively; >2000 trap nights at both sites and classic and spatially explicit capture-recapture models with the sex of individuals as a covariate. Density estimates were 2.52±0.46-3.15±1.08 adults/100 km2 in the Magdalena valley, whereas 1.12±0.13-2.19±0.99 adults/100 km2 in the Colombian llanos, depending on analysis used. We suggest that jaguars are able to live across unprotected human-use areas and co-exist with agricultural landscapes including oil-palm plantations if natural areas and riparian habitats persist in the landscape and hunting of both jaguar and prey is limited. In the face of an expanding agriculture across the tropics we recommend land-use planning, adequate incentives, regulations, and good agricultural practices for range-wide jaguar connectivity and survival.

Urban Flood Risk Assessment Under Uncertain Conditions and Scarce Information

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Rodríguez-Gaviria, E. M.; Botero-Fernandez, V.

2015-12-01

Flood risk management in small urban areas in Colombia has a great degree of uncertainty due to the low availability and quality of data, the non-existent personnel qualified in the collection and processing of data, and the insufficient information to evaluate the risk and vulnerability. It is because of this that two methods are developed: one for the generation of flood threat maps for different return periods combining historical, geomorphological, and hydrological hydraulic methods assisted by remote sensors and SIG through the use of data acquired through field campaigns, official hydrological networks, orthophotos, multitemporal topographic maps, and ASTER, STRM, and LiDAR images. And another method in which categorical variables are established, linking local physical, social, economical, environmental and political-institutional factors that are explored through different media such as reports, news, databases, transects, interviews, community workshops, and surveys conducted at homes. Such variables were included within an analysis of multiple correspondence to conduct a descriptive study of the exposure, susceptibility, and capacity conditions and to create a vulnerability index that was spatially plotted spatially on maps. The uncertainty is reduced in the measure in which local knowledge is used as a source of information acquisition, of validation of what already exists, and of calibration of the proposed methods. This research was applied to the urban centers of Caucasia (Antioquia) and Plato (Magdalena), which have been historically affected by slow flooding of the Magdalena and Cauca river, it being especially useful in the selection of best alternatives for risk management, planning for development, and land use management, with the possibility of replicating it to benefit other municipalities that experience the same reality.

Activation of Saccharomyces cerevisiae Mlh1-Pms1 Endonuclease in a Reconstituted Mismatch Repair System*

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Smith, Catherine E.; Bowen, Nikki; Graham, William J.; Goellner, Eva M.; Srivatsan, Anjana; Kolodner, Richard D.

2015-01-01

Previous studies reported the reconstitution of an Mlh1-Pms1-independent 5′ nick-directed mismatch repair (MMR) reaction using Saccharomyces cerevisiae proteins. Here we describe the reconstitution of a mispair-dependent Mlh1-Pms1 endonuclease activation reaction requiring Msh2-Msh6 (or Msh2-Msh3), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) and a reconstituted Mlh1-Pms1-dependent 3′ nick-directed MMR reaction requiring Msh2-Msh6 (or Msh2-Msh3), exonuclease 1 (Exo1), replication protein A (RPA), RFC, PCNA, and DNA polymerase δ. Both reactions required Mg2+ and Mn2+ for optimal activity. The MMR reaction also required two reaction stages in which the first stage required incubation of Mlh1-Pms1 with substrate DNA, with or without Msh2-Msh6 (or Msh2-Msh3), PCNA, and RFC but did not require nicking of the substrate, followed by a second stage in which other proteins were added. Analysis of different mutant proteins demonstrated that both reactions required a functional Mlh1-Pms1 endonuclease active site, as well as mispair recognition and Mlh1-Pms1 recruitment by Msh2-Msh6 but not sliding clamp formation. Mutant Mlh1-Pms1 and PCNA proteins that were defective for Exo1-independent but not Exo1-dependent MMR in vivo were partially defective in the Mlh1-Pms1 endonuclease and MMR reactions, suggesting that both reactions reflect the activation of Mlh1-Pms1 seen in Exo1-independent MMR in vivo. The availability of this reconstituted MMR reaction should now make it possible to better study both Exo1-independent and Exo1-dependent MMR. PMID:26170454

Activation of Saccharomyces cerevisiae Mlh1-Pms1 Endonuclease in a Reconstituted Mismatch Repair System.

Science.gov (United States)

Smith, Catherine E; Bowen, Nikki; Graham, William J; Goellner, Eva M; Srivatsan, Anjana; Kolodner, Richard D

2015-08-28

Previous studies reported the reconstitution of an Mlh1-Pms1-independent 5' nick-directed mismatch repair (MMR) reaction using Saccharomyces cerevisiae proteins. Here we describe the reconstitution of a mispair-dependent Mlh1-Pms1 endonuclease activation reaction requiring Msh2-Msh6 (or Msh2-Msh3), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) and a reconstituted Mlh1-Pms1-dependent 3' nick-directed MMR reaction requiring Msh2-Msh6 (or Msh2-Msh3), exonuclease 1 (Exo1), replication protein A (RPA), RFC, PCNA, and DNA polymerase δ. Both reactions required Mg(2+) and Mn(2+) for optimal activity. The MMR reaction also required two reaction stages in which the first stage required incubation of Mlh1-Pms1 with substrate DNA, with or without Msh2-Msh6 (or Msh2-Msh3), PCNA, and RFC but did not require nicking of the substrate, followed by a second stage in which other proteins were added. Analysis of different mutant proteins demonstrated that both reactions required a functional Mlh1-Pms1 endonuclease active site, as well as mispair recognition and Mlh1-Pms1 recruitment by Msh2-Msh6 but not sliding clamp formation. Mutant Mlh1-Pms1 and PCNA proteins that were defective for Exo1-independent but not Exo1-dependent MMR in vivo were partially defective in the Mlh1-Pms1 endonuclease and MMR reactions, suggesting that both reactions reflect the activation of Mlh1-Pms1 seen in Exo1-independent MMR in vivo. The availability of this reconstituted MMR reaction should now make it possible to better study both Exo1-independent and Exo1-dependent MMR. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Detailed Measurements of Rayleigh-Taylor Mixing at Large and Small Atwood Numbers

International Nuclear Information System (INIS)

Malcolm, J.; Andrews, Ph.D.

2004-01-01

This project has two major tasks: Task 1. The construction of a new air/helium facility to collect detailed measurements of Rayleigh-Taylor (RT) mixing at high Atwood number, and the distribution of these data to LLNL, LANL, and Alliance members for code validation and design purposes. Task 2. The collection of initial condition data from the new Air/Helium facility, for use with validation of RT simulation codes at LLNL and LANL. Also, studies of multi-layer mixing with the existing water channel facility. Over the last twelve (12) months there has been excellent progress, detailed in this report, with both tasks. As of December 10, 2004, the air/helium facility is now complete and extensive testing and validation of diagnostics has been performed. Currently experiments with air/helium up to Atwood numbers of 0.25 (the maximum is 0.75, but the highest Reynolds numbers are at 0.25) are being performed. The progress matches the project plan, as does the budget, and we expect this to continue for 2005. With interest expressed from LLNL we have continued with initial condition studies using the water channel. This work has also progressed well, with one of the graduate Research Assistants (Mr. Nick Mueschke) visiting LLNL the past two summers to work with Dr. O. Schilling. Several journal papers are in preparation that describe the work. Two MSc.'s have been completed (Mr. Nick Mueschke, and Mr. Wayne Kraft, 12/1/03). Nick and Wayne are both pursuing Ph.D.s' funded by this DOE Alliances project. Presently three (3) Ph.D. graduate Research Assistants are supported on the project, and two (2) undergraduate Research Assistants. During the year two (2) journal papers and two (2) conference papers have been published, ten (10) presentations made at conferences, and three (3) invited presentations

The Role of XPG in Processing (CAGn/(CTGn DNA Hairpins

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Hou Caixia

2011-03-01

Full Text Available Abstract Background During DNA replication or repair, disease-associated (CAGn/(CTGn expansion can result from formation of hairpin structures in the repeat tract of the newly synthesized or nicked DNA strand. Recent studies identified a nick-directed (CAGn/(CTGn hairpin repair (HPR system that removes (CAGn/(CTGn hairpins from human cells via endonucleolytic incisions. Because the process is highly similar to the mechanism by which XPG and XPF endonucleases remove bulky DNA lesions during nucleotide excision repair, we assessed the potential role of XPG in conducting (CAGn/(CTGn HPR. Results To determine if the XPG endonuclease is involved in (CAGn/(CTGn hairpin removal, two XPG-deficient cell lines (GM16024 and AG08802 were examined for their ability to process (CAGn/(CTGn hairpins in vitro. We demonstrated that the GM16024 cell line processes all hairpin substrates as efficiently as HeLa cells, and that the AG08802 cell line is partially defective in HPR. Analysis of repair intermediates revealed that nuclear extracts from both XPG-deficient lines remove CAG/CTG hairpins via incisions, but the incision products are distinct from those generated in HeLa extracts. We also show that purified recombinant XPG protein greatly stimulates HPR in XPG-deficient extracts by promoting an incision 5' to the hairpin. Conclusions Our results strongly suggest that 1 human cells possess multiple pathways to remove (CAGn/(CTGn hairpins located in newly synthesized (or nicked DNA strand; and 2 XPG, although not essential for (CAGn/(CTGn hairpin removal, stimulates HPR by facilitating a 5' incision to the hairpin. This study reveals a novel role for XPG in genome-maintenance and implicates XPG in diseases caused by trinucleotide repeat expansion.

Technology

Science.gov (United States)

Breakstone, Elizabeth

2008-01-01

This column presents an interview with Nick Baker, Reference and Web Services librarian at Williams College. Baker has produced several films for and about the library profession. In 2007, he won an InfoTubey Award for Outstanding Library YouTube Productions for his work. In this interview, Baker shares his ideas and experiences about this…

Student Engagement, Ideological Contest and Elective Affinity: The Zepke Thesis Reviewed

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Trowler, Paul

2015-01-01

This paper takes up issues raised in two articles by Nick Zepke and portrayed here as "the Zepke thesis". This thesis argues that the literature on, interest in and practices around student engagement in higher education have an elective affinity with neo-liberal ideology. At one level this paper counters many of the assertions that…

Environmental education for hazardous waste management and risk reduction in laboratories

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Tomas Rafael Pierre Martinez

2013-10-01

Full Text Available The University laboratories are places where teaching, extension and research activities are develop, which harmful substances are manipulated and hazardous waste are generated, the lack of information about this makes them an inadequate provision causing human health and environmental risks. This research proposes the implementation of environmental education as an alternative for waste management and safety in the University of Magdalena laboratories. Applying a series of polls showed the effectiveness with efficiency or assertively rises at 30% cognitive level during the process. It recommends to obtain better results is necessary evaluate the ethic component.  

Change at the top for the CERN physics schools

CERN Multimedia

2009-01-01

After directing the CERN physics schools since 1993, Egil Lillestøl has handed over to Nick Ellis. At the same time, Hélène Haller has taken over from Danielle Métral as the schools’ administrator. From left to right: Nick Ellis, Hélène Haller, Danielle Métral and Egil Lillestøl.The CERN physics schools for young experimentalists date back to the 1960s and as early as 1971 collaboration with the Joint Institute for Nuclear Research (JINR) in Dubna led to the Joint CERN-JINR schools, which reached beyond CERN’s Member States every two years. Then, in 1993, CERN and JINR agreed to organize the schools jointly every year, as the European School for High-Energy Physics. Egil Lillestøl, has not only run this school very successfully since then, but also created the CERN Latin American School of High-Energy Physics, beginning in 2001. Danielle Métral has been responsible for the schools’ administration since 2001, both for t...

Quantification and genome-wide mapping of DNA double-strand breaks.

Science.gov (United States)

Grégoire, Marie-Chantal; Massonneau, Julien; Leduc, Frédéric; Arguin, Mélina; Brazeau, Marc-André; Boissonneault, Guylain

2016-12-01

DNA double-strand breaks (DSBs) represent a major threat to the genetic integrity of the cell. Knowing both their genome-wide distribution and number is important for a better assessment of genotoxicity at a molecular level. Available methods may have underestimated the extent of DSBs as they are based on markers specific to those undergoing active repair or may not be adapted for the large diversity of naturally occurring DNA ends. We have established conditions for an efficient first step of DNA nick and gap repair (NGR) allowing specific determination of DSBs by end labeling with terminal transferase. We used DNA extracted from HeLa cells harboring an I-SceI cassette to induce a targeted nick or DSB and demonstrated by immunocapture of 3'-OH that a prior step of NGR allows specific determination of loci-specific or genome wide DSBs. This method can be applied to the global determination of DSBs using radioactive end labeling and can find several applications aimed at understanding the distribution and kinetics of DSBs formation and repair. Copyright © 2016 Elsevier B.V. All rights reserved.

A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor.

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Mao, Yu; Chen, Yongli; Li, Song; Lin, Shuo; Jiang, Yuyang

2015-11-09

A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand (CPDNA) that partially hybridizes to the DNA biosensor. Then, the polymerization/nicking enzyme synergetic isothermal amplification could be triggered, leading to the synthesis of massive DNA amplicons, thus achieving an enhanced sensitivity with a wide linear dynamic response range of four orders of magnitude and good selectivity. This biosensing strategy expands the applications of GO-DNA nanobiointerfaces in biological sensing, showing great potential in fundamental research and biomedical diagnosis.

Evaluación de métodos de marcación-radioactivos y no radioactivos empleados en la elaboración de sondas diagnósticas de malaria

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Gladys Pinilla

1993-09-01

Full Text Available Se probaron métodos de marcación radioactivos dentro de los cuales el sistema Random Primers resultó mejor que el de Nick Translation. Se obtuvieron sondas con mayor actividad especifica (1-2X1O9dpmIug y una sensibilidad 10 veces mayor en la detección de ADN de Plasmodium falciparum. La sensibilidad alcanzada (12pg obtenida con la sonda PFCOL692 desarrollada y caracterizada en nuestro laboratorio, fue igual a la obtenida con la sonda pRepHind, una de las más utilizadas en los laboratorios de otros países. El método de marcación no radioactivoconfotobiotina mostró ventajas sobre la marcación por Nick Translation con nucleótidos biotinilados y se alcanzó una sensibilidad de 135 pg de ADN de P. falciparum. También se probó un sistema degeneración de señales fluorescentes mediante el empleo de proteasas, y los resultados preliminares muestran sus posibilidades como método de detección.

"Esst estnische Pasteten in estnischen Nischen" : Ilmar Laabans poetische Welt / Gisbert Jänicke

Index Scriptorium Estoniae

Jänicke, Gisbert, 1937-

2000-01-01

Artikkel sisaldab I. Laabani luuletusi saksa k.: Das Ende der Ankerkette ist des Gesanges Beginn ; Ich liebe euch ; "Die Quelle des Tods in sauberen Wolken..." ; Vögel des Selbstmords ; "Ich zerstreue mich über meinen Tisch..." ; Pornografisch ; Die Moral des Nordlichts ; Mikrokosmos 1, 20, 23, 26, 29, 30, 34, 49. Samuti sisaldab artikkel I. Laabani rootsik. luuletuse "Hur står det till?" saksak. teksti ja I. Laabani saksa k. kirjutatud luuletuse "Funkenspielstück".

Zehn Oden = Kümme oodi / Kristjan Jaak Peterson ; Deutsch von Gisbert Jänicke

Index Scriptorium Estoniae

Peterson, Kristjan Jaak, 1801-1822

2001-01-01

Sisu: An Gott ; Sonnenuntergang ; Herbst ; Der Mensch ; Der Mond ; Freundschaft ; Ich muss trinken. Liedchen ; Hoffnung ; Ich bin wieder glücklich ; Der Sänger. Orig.: Jummalale ; Päva loja-minneminne ; Süggise ; Innimenne ; Kuu ; Söbradus ; Ma pean joma. Lauloke ; Lotus ; Ollen jälle önnis ; Laulja

Tabellae et tabulata : [luuletused] / Hasso Krull ; aus dem Estnischen von Gisbert Jänicke

Index Scriptorium Estoniae

Krull, Hasso, 1964-

2001-01-01

Sisu : Der Kopf dreht sich [Pea käib ringi] ; Gedicht [Luuletus] ; Vier Tafeln 1-4 [Neli tabelit 1-4] ; I : "Gerade hier entlang kamen wir in den Verkehr..." [I : "Just siitkaudu tulime liikumisele lähedale..."] ; II : "Angenehmer Kaffeeduft. Von einer Stärke, die..." [II : "Meeldiv kohvilõhn. Sellel on jõudu, mis toob..."] ; III : "Hesekiel schreibt, "ihre Felgen waren so hoch, dass ich..." [III : "Hesekiel märgib, et "nende pöiad olid kõrged ning..."] ; IV : "Zwangsläufig wird der Luftdruck immer spürbarer..." [IV : "Paratamatult muutub õhurõhk üha märgatavamaks ..."] ; V : "Ich war im Wald, sammelte Blätter von den Bäumen, ass sie..." [V : "Olin metsa all, korjasin puulehti, sõin neid..."] ; VI : "Der Grund für die Gewalt ist immer der andere. Der..." ["Vägivalla alus on just üks Teine. Kes..."] ; - : "Grüsst euch. Euch auch..." [-: "No tere. Tere ka..."

Applying ARIMA model for annual volume time series of the Magdalena River

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Gloria Amaris

2017-04-01

Conclusions: The simulated results obtained with the ARIMA model compared to the observed data showed a fairly good adjustment of the minimum and maximum magnitudes. This allows concluding that it is a good tool for estimating minimum and maximum volumes, even though this model is not capable of simulating the exact behaviour of an annual volume time series.

Caracterización de la diversidad genética en el pez Brycon henni (Characiformes: Characidae en Colombia central por medio de marcadores RAPD

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Hermes Pineda Santis

2007-12-01

Full Text Available El conocimiento sobre la diversidad genética de especies nativas de peces, es esencial para la conservación y manejo apropiado de animales en los programas de repoblación. Brycon henni ha sido reportada en las cuencas de los ríos Magdalena y Cauca; actualmente, la especie ha disminuido su número de animales y reducido su distribución geográfica como consecuencia de los efectos antrópicos. Por lo tanto, es necesario conocer el componente genético de los reducidos grupos de animales en los riachuelos, para iniciar algunos programas de repoblación. En este estudio el Polimorfismo de ADN Amplificado al Azar (RAPD, fue utilizado para estimar el componente genético actual en esta especie. Para este propósito; se evaluaron seis sitios de muestreo localizados en el departamento de Antioquia, cordillera Central de Colombia. De cuarenta iniciadores utilizados, treinta y cinco de ellos (87.5 % produjeron 1 466 fragmentos reproducibles y consistentes; 417 fueron considerados como fragmentos únicos, que permitieron discriminar entre las muestras de las cuencas de los ríos Magdalena (Humarada-1 y Humarada-2 y Cauca (Piedras, La Clara y Guaracú, sugiriendo que cada una es una unidad discreta. Esta diversidad en los resultados, según el sitio de muestreo y por las características de cada uno de ellos, posiblemente sugiere, que los efectos antrópicos como presión por pesca, construcción de embalses, deforestación y contaminación del agua, han contribuido al aislamiento de estos grupos de peces en las zonas de alta montaña. Brycon moorei y Colossoma macropomum, como grupos de control inter especifico, se ubicaron fuera del grupo general de B. henni, confirmando su clasificación taxonómica mediante datos morfológicos. La técnica de RAPD fue útil para conocer la diversidad genética y discriminar entre poblaciones de B. henni de diferente origen geográfico, ello permitiría realizar un plan apropiado de conservación y manejo en medio

The Pedagogue of the Auratic Medium--Extending the Argument

Science.gov (United States)

Dobson, Stephen

2008-01-01

Nick Peim has recently revisited the work of Walter Benjamin; specifically his famous essay on art and mechanical reproduction. In this reply, I too draw upon the inspiration of Benjamin to extend the argument to the question of experience and what might count as knowledge, both in a philosophical sense and also in terms of the curriculum. To…

Before Coffee, Facebook: New Literacy Learning for 21st Century Teachers

Science.gov (United States)

Roach, Audra K.; Beck, Jessica J.

2012-01-01

And so a middle school language arts teacher begins her Saturday. Before coffee, Facebook. In this themed issue on professional development in an era of nick.com, the authors propose that teachers' new literacy learning is as close as their own screens. Teachers, too, live literate lives online in this new age of composition, with impulses to…

Pop & rock. Retro. Klassika / Margus Kiis

Index Scriptorium Estoniae

Kiis, Margus

2001-01-01

Orbital "The Altogether", Vonda Shepard "Ally McBeal (For Once In My Life)", Jääboiler "Niiongi", Tanel Padar ja Speed Free "Woman Knows", Arvo Pärt "Johannes-Passion", Nikka Costa "Everybody Got Their Something", Stevie Nicks "Trouble In Shangri-La", Richard Thompson "Action Packed The Best of the Capitol Years", "Eurovision Song Contest 2001" heliplaatide tutvustused

The Significance of Learning Nicknames of Public Figures in Modern English and American Language Models of the World

Science.gov (United States)

Garayeva, Almira K.; Akhmetzyanov, Ildar G.; Khismatullina, Lutsia G.

2016-01-01

The importance of the topic of this study is determined by several factors: increased interest of linguists to the problem of interaction between language and culture; the need to study the onomastic units as body language. The purpose of this article is to identify the types of motivational nick names of famous American and English public…

Tänavuse Berlinale filmid süvenevad ihulähedasse / Grete Nootre

Index Scriptorium Estoniae

Nootre, Grete

2006-01-01

56. Berliini filmifestivali programmidest ja filmidest - USA mängufilm "A Prairie Home Companion" : režissöör Robert Altman, saksa mängufilm "Vaba tahe" ("Der Freie Wille") : režissöör Matthias Glasner, austraalia-inglise "Ettepanek" ("The Proposition") : stsenarist ja helilooja Nick Cave : režissöör John Hillcoat

Healthcare reform. Is the NHS ready for US business guru's strategy?

Science.gov (United States)

Cavendish, Will; Edwards, Nigel; Swindells, Matthew; Henke, Nicolaus; Robinson, Edna; Smith, Richard

2006-12-07

The central argument of the new book by renowned US academics Michael Porter and Elizabeth Olmsted Teisberg is that the US health system is broken because rather than improving quality and efficiency, it focuses on budgetary battles. HSJ gathered together six leading healthcare insiders to discuss whether his diagnosis is applicable to the NHS. Nick Edwards was there.

DISTRIBUCIÓN POTENCIAL DE Podocnemis lewyana (REPTILIA:PODOCNEMIDIDAE Y SU POSIBLE FLUCTUACIÓN BAJO ESCENARIOS DE CAMBIO CLIMÁTICO GLOBAL

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Carlos Ortiz-Yusty

2014-09-01

Full Text Available En este estudio se implementó el modelaje de distribución de especies para establecer el rango de distribución potencial de Podocnemis lewyana, explorar los componentes del clima que pueden influenciar dicha distribución y evaluar posibles fluctuaciones de su distribución bajo escenarios de clima futuro. Los modelos obtenidos predicen una distribución continua de sur a norte por todo el río Magdalena, desde los municipios de Rivera y Palermo en el departamento de Huila, hasta los departamentos de Atlántico y Magdalena en el norte. La temperatura fue el elemento del clima que más influyó en la distribución de P. lewyana; esta especie tiende a estar presente en climas cálidos y con poca variabilidad en la temperatura. El modelo de distribución transferido a los escenarios de clima futuro predicen un aumento en el rango geográfico de P. lewyana. Sin embargo, teniendo en cuenta las preferencias de hábitat de esta especie y su fuerte asociación con los cuerpos de agua, este resultado debe tomarse con cautela, dado que el modelo solo tuvo en cuenta variables climáticas terrestres. Dadas las características de historia de vida de esta especie (presencia de determinación sexual dependiente de la temperatura, alta temperatura pivotal y un rango de transición de temperatura muy estrecho, y el efecto negativo de los cambios en los regímenes hidrológicos en la mortalidad embrionaria, la expansión del área potencial de P. lewyana en el futuro no significa que esta especie no vaya a verse afectada por el cambio climático global.

Sordera, tragedia y muerte de Ludwig van Beethoven

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Darío Morón Díaz

2008-07-01

Full Text Available

La sordera de Ludwig van Beethoven fue atribuida a distintas enfermedades: a una neuritis
acústica debido a la fiebre tifoidea, a la sífilis, a la hepatitis, a una colitis, al reumatismo, a abscesos,
a una criopatia debida al frío y a la osificación del tejido cartilaginoso que conduce al oído ínterno,
también mencionaron la sarcoidosis.
Dos hechos fueron fundamentales para desechar tales especulaciones: el primero, la decisión del
joven Ferdinand Hiller, que el 27 de marzo de 1827 cortó un mechón de cabello del cadáver de Ludwig
van Beethoven. El segundo, el avance científico de la medicina moderna. Ambos acontecimientos han
permitido profundizar con certeza en la etiología u origen de la sordera de Beethoven y la variada síntomatología que sufrió el genio musical.

Esbozo biográfico
EL 16 de diciembre de 1770, en Bonn, nació Ludwig van Beethoven, hijo de Johann Beethoven y
Maria Magdalena Keverich; el matrimonio tuvo siete hijos, cuatro fallecieron siendo niños y tres sobrevivieron: Ludwig, Kaspar Antón Karl y Nicolás Johann.
El padre de Beethoven fue dipsómano, debido a los excesos alcohólicos y en medio de los escándalos el
padre agredía al niño. Deambulaba entre la Iglesia y la taberna. María Magdalena por el contrario era tierna y afectuosa con su hijo, situación que por contraste servía de refugio a Ludwig.

Current State of Knowledge of the Concentration of Mercury and Other Heavy Metals in Fresh Water Fish in Colombia

Directory of Open Access Journals (Sweden)

Néstor Javier Mancera-Rodríguez

2006-01-01

Full Text Available One of the most important environmental problems in the country refers to the indiscriminate use of chemical precursors in illicit activities, the use of heavy metals as mercury in mining activities, the spill of served waters and another type of compound related with the industrial activities of raw and the inadequate agricultural practices. This has led to chemical contamination especially by heavy metals, considered one of the most dangerous for the aquatic ecosystems and the present species in them. Fish have the capacity to store these compounds in their organism in a concentration higher important indicators of the contamination level, but also this implies that their consumption can become a serious health problem for the populations that feeds from them. The concentration of heavy metals in fish of fresh water is better known in the basin of the Magdalena river, especially in the region of the Mojana and in the marshes of the south of the Department of Bolivar where the levels of contamination by mercury and other metals has been studied due to the development of multiple industrial activities, including gold mining and petrochemical industries. However, little is known in the country about the problem generated by the disposal heavy metals in rivers and lakes and their impact on the fish resource, deterioration of ecosystems and human health. Based in the current norms bio-assays have been used to check the effects of the aquatic contamination on fresh waters fish and the evaluation of at least three parameters (heavy metals, temperature, effluents in eight species of fresh waters fish: Carassius auratus, Oreochromis spp., Piractus brachypomus, Prochilodus magdalenae, Astyanax fasciatus, Colossoma bidens, Gambusia affinis and Grundulus bogotensis.

Current state of knowledge of the concentration of mercury and other heavy metals in fresh water fish in Colombia

International Nuclear Information System (INIS)

Mancera Rodriguez, Nestor Javier; Alvarez Leon, Ricardo

2006-01-01

One of the most important environmental problems in the country refers to the indiscriminate use of chemical precursors in illicit activities, the use of heavy metals as mercury in mining activities, the spill of served waters and another type of compound related with the industrial activities of raw and the inadequate agricultural practices. This has led to chemical contamination especially by heavy metals, considered one of the most dangerous for the aquatic ecosystems and the present species in them. Fish have the capacity to store these compounds in their organism in a concentration higher than that in the surrounding environment (water), therefore, their concentration are important indicators of the contamination level, but also this implies that their consumption can become a serious health problem for the populations that feeds from them. The concentration of heavy metals in fish of fresh water is better known in the basin of the Magdalena river, especially in the region of the Mojana and in the marshes of the south of the department of Bolivar where the levels of contamination by mercury and other metals has been studied due to the development of multiple industrial activities, including gold mining and petrochemical industries. However, little is known in the country about the problem generated by the disposal heavy metals in rivers and lakes and their impact on the fish resource, deterioration of ecosystems and human health. Based in the current norms bio-assays have been used to check the effects of the aquatic contamination on fresh waters fish and the evaluation of at least three parameters (heavy metals, temperature, effluents) in eight species of fresh waters fish: carassius auratus, oreochromis spp., piractus brachypomus, prochilodus magdalenae, astyanax fasciatus, colossoma bidens, gambusia affinis and grundulus bogotensis

Morfología de la semilla y anatomía de la cubierta seminal de cinco especies de Calliandra (Leguminosae-Mimosoideae de Venezuela

Directory of Open Access Journals (Sweden)

Sirli Leython

2008-09-01

Full Text Available Se estudió la morfología de las semillas y la anatomía de la cubierta seminal en cinco especies venezolanas del género Calliandra, con la finalidad de establecer similitudes y diferencias entre estos taxa, para su utilización como criterio taxonómico. Se analizaron caracteres morfológicos de valor taxonómico; entre ellos la forma y el tamaño de la semilla, así como anatómicos tales como espesor de las diferentes regiones, la ubicación de la línea lúcida y el tamaño relativo de los espacios intercelulares en la hipodermis. Con los resultados se realizaron análisis de agrupamiento por medio de la función de análisis discriminante. Diez caracteres cuantitativos, y tres caracteres cualitativos, resultaron informativos para la separación de especies.Seed morphology and anatomy of the seed coat in five species of Calliandra (Leguminosae-Mimosoideae from Venezuela. Calliandra is an exclusively Neotropoical genus with 135 described species. There are 30 species in Venezuela. We studied seed morphology and the seed coat anatomy of five species: Calliandra riparia Pittier, Calliandra glomerulata Karsten var. glomerulata, Calliandra magdalenae (DC. Benth. var. magdalenae, Calliandra surinamensis Benth. and Calliandra falcata Benth., to establish simililarities and differences amongst taxa for taxonomic characterization. Of the evaluated characters, 10 quantitative characters and 3 qualitative characters were selected as the most informative for the separation of species. Seed morphological characters of taxonomic value are form and size. Thickness of the light line, number of layers and space between the osteosclereids constitute distinctive characters. Rev. Biol. Trop. 56 (3: 1075-1086. Epub 2008 September 30.

Inteligencia emocional y ansiedad en estudiantes universitarios

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Ubaldo Rodríguez de Ávila

2011-07-01

Full Text Available Abstract This research paper presents the results of a study about the relationship between Perceived Emotional Intelligence and the State-Trait Anxiety psychology students in a public university in Magdalena, Colombia. The investigation was developed from the descriptive-correlational design through the application of the instruments TMMS-24 and INDARE to 167 subjects. The results indicated that there is direct and significant relationship between emotional perception and state anxiety and trait anxiety. By contrast, there was a significant inverse relationship between emotional understanding and state anxiety and trait anxiety, and also between emotional regulation and state anxiety and trait anxiety. Through statistical analysis with Chi2, dependence was also found between the sub-variables with a significance level less than 0.05. Resumen El presente artículo de investigación presenta los resultados de un estudio acerca de la relación entre la Inteligencia Emocional Percibida y la Ansiedad Rasgo-Estado en estudiantes de Psicología de una universidad pública del Magdalena, Colombia. La investigación se desarrolló a partir del diseño descriptivo-correlacional a través de la aplicación de los instrumentos TMMS-24 y el IDARE a 167 sujetos. Los resultados indicaron que existe relación directa y significativa entre la percepción emocional y la ansiedad estado y la ansiedad rasgo. Por el contrario, se presentó una relación inversa y significativa entre la comprensión emocional y la ansiedad estado y la ansiedad rasgo; igualmente entre la regulación emocional y la ansiedad estado y la ansiedad rasgo. A través del análisis con el estadístico Chi2, también se encontró dependencia entre las sub-variables estudiadas con un nivel de significancia menor de 0.05.

UNA MIRADA A LA VIOLENCIA QUE LEEN LOS SAMARIOS

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Grace Angeline Morales Urueta

2011-01-01

Full Text Available Resumen Esta investigación, de carácter cuantitativo, se orientó a observar la violencia en el contenido de las noticias locales del Distrito de Santa Marta publicadas en los diarios: El Informador, Hoy Diario del Magdalena, Ajá y Qué y Al Día. El análisis caracterizó la violencia Directa, Cultural y Estructural en los espacios en los que se desarrolla: Naturaleza, Hombre y Sociedad, de acuerdo a la Teoría sobre las estructuras de violencia en la sociedad de Johan Galtung; para el análisis de la estructura del texto se utilizó el planteamiento de Teun Van Dijk. Los resultados indican que los hechos de violencia se registraron en mayor proporción en el espacio de los Hombres con actos que tipifican Violencia directa; seguidamente la Sociedad con hechos de Violencia estructural y en el escenario de la Naturaleza acciones violentas de tipo cultural. Abstract This quantitative research was aimed to observe the level of violence in the local news content of the District of Santa Marta, published in newspapers such as: El Informador, Hoy Diario del Magdalena, Ajá y Qué, and Al Día. The analysis characterized the cultural and structural direct violence among the spaces where it usually develops: Nature, Man and Society, according to the theory about the structures of violence in society by Johan Galtung, for the analysis of text structure the approach of Teun Van Dijk was used. Results indicate that the violent events that occurred in greater proportion were observed in environments of humans whose acts constitute direct violence, followed by society, whose acts constitute structural violence, and finally nature, whose acts constitute cultural violence.

Estudios genéticos en las comunidades indígenas del nororiente colombiano

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Humberto Ossa

1994-01-01

Full Text Available Se presentan las frecuencias génicas de los grupos sanguíneos ABO, Rh, Kell, Duffy, Kidd, Diego y MNSs en las siete comunidades que viven en el nororiente columbiano (wayrl, Barf, Arhuaco, Yuco, Kogi, Arsario y Chimila. Además se presenta el índice de mezcla racial, con excepción de los Arsario, y algunos aspectos iniciales de las distancias genéticas, con excepción de los Arsario y los Chimila, sobre la base de cinco loci informativos. Las frecuencias génicas fueron obtenidas a partir de los genotipos deducidos a través de los arboles familiares. Geográficamente el nororiente colombiano se encuentra conformado por los departamentos de la Guajira, Magdalena, Cesar y Norte de Santander. En la península de la Guajira viven los Wayu; en la Sierra Nevada de Santa Marta habitan los Kogi, los Arhuaco y los Arsario, todos descendientes de los primitivos Taironas. En la Serranía del Perija encontramos los Yuco al norte y los Barf al sur y en el departamento del Magdalena encontramos los Chimila. Estos grupos indígenas fueron estudiados para siete sistemas genéticos polimórficos que comprenden 23 alelos y 18 especificidades serológicas: ABO(tres alelos,Rh (seisalelos, Kell (dos alelos,Duffy (tres alelos, Kidd (tres alelos,Diego (dos alelos y MNSs (cuatro alelos. EI estudio se adelanto utilizando anticuerpos policlonales y monoclonales y las técnicas de tipificación convencionales. Se realizaron quince visitas a las siete comunidades y se recolectaron 473 muestras sanguíneas distribuidas en 63 familias de dos, tres y cuatro generaciones. Se presentan los resultados para frecuencias de fenotipos y genotipos en las poblaciones estudiadas y los resultados del análisis del equilibrio de Hardy-Weinberg.

Realismist animatsioonfilmis / Ülo Pikkov

Index Scriptorium Estoniae

Pikkov, Ülo, 1976-

2007-01-01

Animafilmist kui realistlikust filmiliigist. Artiklis viited ka mitmetele filmiteoreetikutele nagu Juri Lotman, Martin Heidegger, Steve Reinke, Paul Wells, Stephen Rowley. Näited filmidest "Lusitania uppumine" ("The Sinking of theLusitania", Winsor McCay, 1918), "Ryan" (Chris Landherth, 2004), "Creature Comforts" (Nick Park, 1989), "Pocahontas" (1995), "Jurassic Park" (Steven Spielberg, 1993), "Beavis ja Butt-head" (1993-1997), "South Park" (1997-2007)

Analysis of SSN 688 Class Submarine Maintenance Delays

Science.gov (United States)

2017-06-01

Simplified Notional Submarine FRP (Independent Deployer) ..................11  Figure 8.  Evolution of Los Angeles Class Submarine Notional...Number TFP Technical Foundation Paper URO Unrestricted Operations xv ACKNOWLEDGMENTS I would like to thank my lead advisor, Professor Nick Dew...only on Los Angeles (SSN 688)-class submarines. Being the higher quantity and older generation submarine hull type, the Los Angeles class submarine

Contribución al conocimiento de la reproducción de Podocnemis Lewyana Dumeril (Reptilia: Quelonia: Pelomedusidae

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Castaño Mora Olga Victoria

1986-12-01

Full Text Available En Colombia se encuentran 5 especies del Género Podocnemis : P. expansa, P. lewyana, P. sextuberculata , P. unifilis y P. vogli. De estas P. lewyana es la única que se encuentra en la zona Norte de nuestro país y es endémica para la región que abarca las hoyas del Magdalena y del Sinú.La presion human a la que esta sometida Podocnemis lewyana es muy fuerte, esto sumado a su endemismo, hace que su esperanza de supervivencia se reduzca aceleradamente ; los pescadores de la zona son unánimes en manifestar que su número disminuye notablemente, año tras año

Capital cultural versus dom inato: questionando sociologicamente a trajetória musical de compositores e intérpretes brasileiros

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Rita de Cássia Fucci Amato

2008-05-01

Full Text Available The aim of this paper is to analyze sociologically the cultural constitution of the familiar atmosphere of eight Brazilian erudite and popular musicians (Almeida Prado, Carlos Gomes, Chico Buarque, João Bosco, Magdalena Tagliaferro, Milton Nascimento, Tom Jobim e Villa-Lobos. Making use of their statements and biographies as research material, I will analyze their trajectories under the light of the concept of cultural capital, developed by French sociologist Pierre Bourdieu. Therefore, this article delineates the role of the family in setting up the first musical environment of an individual, offering a critical perspective on the idea of musical gift, or innate talent, and suggesting that the development of artistic abilities is socially constructed.

Nuevas observaciones sobre un tercer foco de fiebre petequial en el hemisferio americano

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Luis Patiño Camargo

1941-11-01

Full Text Available Se ha localizado un foco endémico de Rickettsiasis y se ha denominado fiebre petequial de Tobia, en el interior de Colombia, en zona rural calida de la hoya del Magdalena, a 118 kilómetros de Bogotá. La comarca es fértil, pero las condiciones de vida humana son precarias. El índice de mortalidad por la fiebre petequial de Tobia, es de 95%. Ocurre principalmente en individuos de 20 a 29 años de edad, sin distincion de sexos. Hay epidemias familiares. Las gentes considerando que el contagio reside en las chozas y enseres, cuando se repiten las muertes, incineran las viviendas. Abundan ectoparásitos humanos y animales.

DNA Packaging Specificity of Bacteriophage N15 with an Excursion into the Genetics of a Cohesive End Mismatch.

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Michael Feiss

Full Text Available During DNA replication by the λ-like bacteriophages, immature concatemeric DNA is produced by rolling circle replication. The concatemers are processed into mature chromosomes with cohesive ends, and packaged into prohead shells, during virion assembly. Cohesive ends are generated by the viral enzyme terminase, which introduces staggered nicks at cos, an approx. 200 bp-long sequence containing subsites cosQ, cosN and cosB. Interactions of cos subsites of immature concatemeric DNA with terminase orchestrate DNA processing and packaging. To initiate DNA packaging, terminase interacts with cosB and nicks cosN. The cohesive ends of N15 DNA differ from those of λ at 2/12 positions. Genetic experiments show that phages with chromosomes containing mismatched cohesive ends are functional. In at least some infections, the cohesive end mismatch persists through cyclization and replication, so that progeny phages of both allelic types are produced in the infected cell. N15 possesses an asymmetric packaging specificity: N15 DNA is not packaged by phages λ or 21, but surprisingly, N15-specific terminase packages λ DNA. Implications for genetic interactions among λ-like bacteriophages are discussed.

Gedichte aus den Jahren 1968 bis 1975 / Viivi Luik ; tõlk. Gisbert Jänicke

Index Scriptorium Estoniae

Luik, Viivi, 1946-

1999-01-01

Tekst saksa ja eesti keeles. Sisu: Weite = Avarus ; "Wie der Krieg..." = "Otsekui sõda..." ; "es vergehn..." = "hävivad..." ; Das Geheimnis = Saladus ; "Ob auch ich weiss..." = "Kas minagi tean..." ; "Ich redete einmal von Feldern..." = "Rääkisin väljadest ükskord..." ; Andere = Teine ; Der Tag = Päev

Transformation of Nickelalactones to Methyl Acrylate: On the Way to a Catalytic Conversion of Carbon Dioxide

KAUST Repository

Lee, S. Y. Tina

2011-08-26

Mu-nick: The methyl iodide-mediated ring opening of nickelalactones, which can be formed by oxidative coupling of carbon dioxide and ethylene at Ni 0 complexes, induces β-H elimination, producing methyl acrylate in yields of up to 56 %. This reaction is found to be very sensitive to the ligands coordinated to the central nickel atom. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Combating Terrorism: A Case Study of Nigeria Against Boko Haram Terrorist Group

Science.gov (United States)

2014-06-13

Many scholars on terrorism have pointed to a sense of alienation felt by immigrants , particularly those living in Europe, as a driver of terrorism.39...culture most times are different from those of the immigrants . As a result, they become irritated and begin to feel excluded from the society around...158Nick Chiles , “After Rejecting Nigeria’s Amnesty Offer, Boko Haram Continues to Kill,” Atlanta

Coral reefs chronically exposed to river sediment plumes in the southwestern Caribbean: Rosario Islands, Colombia.

Science.gov (United States)

Restrepo, Juan D; Park, Edward; Aquino, Samia; Latrubesse, Edgardo M

2016-05-15

Politicians do not acknowledge the devastating impacts riverine sediments can have on healthy coral reef ecosystems during environmental debates in Caribbean countries. Therefore, regional and/or local decision makers do not implement the necessary measures to reduce fluvial sediment fluxes on coral reefs. The Magdalena River, the main contributor of continental fluxes into the Caribbean Sea, delivers water and sediment fluxes into the Rosario Islands National Park, an important marine protected area in the southwestern Caribbean. Until now, there is no scientific consensus on the presence of sediment fluxes from the Magdalena River in the coral reefs of the Rosario Islands. Our hypothesis is that high sediment and freshwater inputs from the Magdalena have been present at higher acute levels during the last decade than previously thought, and that these runoff pulses are not flashy. We use in-situ calibrated MODIS satellite images to capture the spatiotemporal variability of the distribution of suspended sediment over the coral reefs. Furthermore, geochemical data are analyzed to detect associated sedimentation rates and pollutant dispersion into the coastal zone. Results confirm that turbidity levels have been much higher than previous values presented by national environmental authorities on coral reefs off Colombia over the last decade. During the 2003-2013-period most of the Total Suspended Sediments (TSS) values witnessed in the sampled regions were above 10mg/l, a threshold value of turbidity for healthy coral reef waters. TSS concentrations throughout the analyzed time were up to 62.3mg/l. Plume pulses were more pronounced during wet seasons of La Niña events in 2002-2003, 2007-2008, and 2009-2010. Reconstructed time series of MODIS TSS indicates that coral reef waters were exposed to river plumes between 19.6 and 47.8% of the entire period of analysis (2000-2013). Further analyses of time series of water discharge and sediment load into the coastal zone

Territorio, Enfermedad y población en la producción da la Geografìa tropical colombiana, 1872-1934 *

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Álvaro Andrés Villegas Vélez

2006-12-01

Full Text Available The Colombian geographic imaginary has condensed into a series of representations that emphasize the tropical character of the nation and at the same time qualify it through a juxtaposition with the Andean region, which has been stripped of that character. In this article, we suggest that three axes frame the discussion of the tropics: nature, disease, and population. We analyze these axes in three different cases that are signifcant for being both tipical and singular: descriptions of Amazonian nature; medical geographies of disease in the Magdalena river valley; and the narratives regarding the Black population of the Colombian Pacifc region. In all these cases, the struggle for territorial appropriation is tied to the desire to know and defne it.

Study of gases in six geologic faults in the Narino Department

International Nuclear Information System (INIS)

Moran Rodriguez, C; Chica Sanchez, A; Garzon Valencia, G

2001-01-01

Radon-222 and Carbon Dioxide emissions on the Manchabajoy, Pasto, Buesaco, Guaitara, Ancuya and Magdalena geological faults in the Narino Department were studied. As example of environmental influences like rainfall on the gas emissions in the San Francisco station, located in the Manchabajoy, Ancuya and Guaitara faults intersection was presented. Another environmental perturbations, like air temperature and atmospheric pressure no varies too much and minimum influence gas emissions from soils. In the first semester of the 2000, several radon anomalies in six stations of the volcanological and seismological observatory of Pasto were registered. After cited Radon anomalies inhabitants of Pasto city filled a seismic swarm in July months, and probably a correlation between radon anomalies and detected seismic signals have to be taken into account

Study of gases in six geologic faults in the Department of Narino

International Nuclear Information System (INIS)

Moran Rodriguez, C; Chica Sanchez, A; Garzon Valencia, G

2001-01-01

Radon-222 and carbon dioxide emissions on the Manchabajoy, Pasto, Buesaco, Guaitara, Ancuya and Magdalena geological faults in the Narino Department were studied. As example of environmental influences like rainfall on the gas emissions in the San Francisco Station, located in the Manchabajoy, Ancuya and Guaitara faults intersection was presented. Another environmental perturbations, like air temperature and atmospheric pressure no varies too much and minimum influence gas emissions from soils. In the first semester of the 2000, several radon anomalies in six stations of the volcanological and seismological observatory of Pasto were registered. After cited radon anomalies a seismic swarm were filled by inhabitants of Pasto City in July months, and probably a correlation between radon anomalies and detected seismic signals have to be taken into account

LIDERAZGO FORMATIVO Y DESARROLLO SOSTENIBLE DE LA CALIDAD DE LA EDUCACIÓN

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Álvaro Rodríguez Akle

2009-01-01

Full Text Available Este artículo contiene los resultado de una investigación cualitativa acerca de los procesos institucionales que contribuyen a la promoción del liderazgo formativo que conduzca a un cambio sostenible de la calidad de la educación en las organizaciones escolares en el marco de su contexto sociocultural. La selección de este caso se realiza atendiendo la capacidad de innovación, adaptación y los procesos de desarrollo de la calidad de la educación y liderazgo sostenida por la institución seleccionada durante un largo período, reconocida por la comunidad y el gobierno distrital de Santa Marta, departamental del Magdalena y nacional de Colombia.

Automatic solar image motion measurements. [electronic disk flux monitoring

Science.gov (United States)

Colgate, S. A.; Moore, E. P.

1975-01-01

The solar seeing image motion has been monitored electronically and absolutely with a 25 cm telescope at three sites along the ridge at the southern end of the Magdalena Mountains west of Socorro, New Mexico. The uncorrelated component of the variations of the optical flux from two points at opposite limbs of the solar disk was continually monitored in 3 frequencies centered at 0.3, 3 and 30 Hz. The frequency band of maximum signal centered at 3 Hz showed the average absolute value of image motion to be somewhat less than 2sec. The observer estimates of combined blurring and image motion were well correlated with electronically measured image motion, but the observer estimates gave a factor 2 larger value.

Exploring the Molecular Mechanisms of Nickel-Induced Genotoxicity and Carcinogenicity: A Literature Review

OpenAIRE

Cameron, Keyuna S.; Buchner, Virginia; Tchounwou, Paul B.

2011-01-01

Nickel, a naturally occurring element that exists in various mineral forms, is mainly found in soil and sediment, and its mobilization is influenced by the physicochemical properties of the soil. Industrial sources of nickel include metallurgical processes such as electroplating, alloy production, stainless steel, and nickel-cadmium batteries. Nickel industries, oil- and coal-burning power plants, and trash incinerators have been implicated in its release into the environment. In humans, nick...

JPRS Report, East Europe.

Science.gov (United States)

1990-01-17

21 Oct] ............................................. 38 THM Dal Plans Clothing Goods Store Franchises [RYNKI ZAGRANICZNE 7 Oct...former classmate Radomir Mladenovic, nick- General Dobrnjac was supposed to have lived. All we named Duce, the owner of the Boter cafe . had left was a... cafe . I know that he would stayed with their mother in Pozarevac. Slobodan not like it, even though he is an idol for many people Milosevic’s father

Still a Special Relationship? The Significance of United States-United Kingdom Relations in the Twenty-First Century

Science.gov (United States)

2011-12-01

Anglo - Saxon ally over Nasser and the canal.55 In 2010, in a speech at Chatham House, Liberal Democrat Nick Clegg suggested that “what...strategic culture, and as an expression of shared values and institutions the character of which is vital for those charged with service in an Anglo ...American context as well as NATO. 14. SUBJECT TERMS United Kingdom, United States, Special Relationship, Anglo - American Relations, Iraq, Afghanistan

A Mitochondria-Dependent Pathway Mediates the Apoptosis of GSE-Induced Yeast

OpenAIRE

Cao, Sishuo; Xu, Wentao; Zhang, Nan; Wang, Yan; Luo, YunBo; He, Xiaoyun; Huang, Kunlun

2012-01-01

Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) ...

CRITICAL MASS

Institute of Scientific and Technical Information of China (English)

DAVID; DAWSON

2015-01-01

The Middle Kingdom’s booming Christian community celebrates Christmas教会里的圣诞节并不是购物与狂欢Worldwide,there are two kinds of Christmas.While both are a time of joy,one is relatively secular;its trappings are Christmas trees,gifts,reindeer,and the jolly,red,fat version of Saint Nick(going by圣诞老人,or Christmas Elderly Person in China).

Effects of TEL Confusers on Operator Target Acquisition Performance with SAR Imagery

Science.gov (United States)

1998-12-01

efforts of everyone involved in the project; and Dr. Nick Patton, for providing assistance with several of the mathematical procedures in the document...processing known as the theory of signal detection (TSD) (Gescheider, 1985; Green & Swets, 1966; Macmillan & Creelman , 1991; Wilson, 1992). A TSD...localizations (Hacker & Ratcliff, 1979; Macmillan & Creelman , 1991). The index of bias in a target localization task provides a measure of the operator’s

The Prado Dam and Reservoir, Riverside and San Bernardino Counties, California

Science.gov (United States)

1989-10-31

County’s Renewed Push for Water Conservation ............. 72 Riverside County Reaction , Late 1940s ........................... 76 Development of...is sloped to the typography to reduce erosion below the concrete-lined section. The emergency spillway had a designed pond elevation of 556 feet, and a...means of pumping water downstream (Nick Richardson, personal communication 1989). 75 4R CL 44- t,, v I. 76 Riverside County Reaction , Late 1940s The

Tiger Rag na interpretação do Le Quintette du Hot Club de France: história, análise e práticas de performance Tiger Rag as performed by the Quintet of the Hot Club of France: history, analysis and performance practices

Directory of Open Access Journals (Sweden)

Adriana Costa

2011-06-01

Full Text Available Estudo sobre a assimilação do jazz na cultura popular francesa dos anos de 1930 e criação de uma sociedade de entusiastas desse estilo, especialmente em torno do Le Quintette du Hot Club de France. A transcrição e análise de Tiger Rag, música dos membros da Original Dixieland Jazz Band (Nick La Rocca, Eddie Edwards, Tony Sbarbaro, Larry Shields e Harry da Costa na interpretação do Le Quintette (LE QUINTETTE DU HOT CLUB DE FRANCE, 1934, remasterizado em 1993 revela suas práticas de performance, especialmente de seus solistas: Django Reinhardt, no violão, e Stephane Grappelli, no violino.Study about the assimilation of jazz into French popular culture of the 1930s and the emergence of a society of jazz enthusiasts, especially around Le Quintette du Hot Club de France. The transcription and analysis of Tiger Rag, composed by the members of the Original Dixieland Jazz Band (Nick La Rocca, Eddie Edwards, Tony Sbarbaro, Larry Shields and Harry da Costa and performed by Le Quintette reveal performance practices of its main soloists: Django Reinhardt on the guitar and Stephane Grappelli on the violin.

Factors affecting mutational specificity in mammalian cells: [Informal technical progress report], February 1, 1986-January 31, 1987

International Nuclear Information System (INIS)

Strauss, B.S.

1987-01-01

We analyzed the different c-H-ras mutations produced in cells after treatment with chemical carcinogens. The overall goal of this work is an understanding of the changes produced by environmental mutagens and carcinogens to learn how closely the results obtained with bacterial and cellular assay systems apply to the in vivo situation. Our work continues to utilize the technique for the study of DNA synthesis termination in vitro developed in this laboraory and utilized for the past several years. This technology can be characterized as a Sanger dideoxy sequencing technique with mutation induced lesions in the template strand serving as chain terminators instead of the dideoxynucleotides used in the Sanger technique to terminate the growing strand. We have added to this technique a modification for the study of termination on double stranded templates as possibly modeling more closely the natural situation. For this modification, double stranded M13 DNA is split once with a restriction enzyme which makes a unique cut and the linearized DNA is hybridized with single stranded circular DNA to make a uniquely nicked double stranded circle with a single 3'OH to serve as a primer for DNA synthesis which can occur by either strand displacement or nick translation

Colliding muons

International Nuclear Information System (INIS)

Anon.

1994-01-01

Is a muon-muon collider really practical? That is the question being asked by Bob Palmer. Well known in particle physics, Palmer, with Nick Samios and Ralph Shutt, recently won the American Physical Society's Panofsky Prize for their 1964 discovery of the omega minus. As well as contributing to other major experiments, both at CERN and in the US, he has contributed ideas to stochastic cooling and novel acceleration schemes

Naval Surface Forces Real-Time Reutilization Asset Management Warehouses: A Cost-Benefit Analysis

Science.gov (United States)

2008-12-01

shared their valuable insights. And finally, to our advisors Dr . Ken Euske and CDR Brett Wagner who kept us on track throughout the project. Nick could...of $206,368,657. SURFOR’s East and West Coast warehouses accounted for $146,975,108 of the list value. The team identified potential cost...obligating the funds. A relatively simple cost benefit analysis of the reprogramming costs versus the cost savings would justify the expense. In the

Drug and Alcohol Studies (Volume 5: Interventions)

OpenAIRE

MacGregor, S; Thom, B

2014-01-01

VOLUME FIVE: INTERVENTIONS Natural Recovery from Alcohol Problems Harald Klingemann School-Based Programmes to Prevent Alcohol, Tobacco and Other Drug Use Gilbert Botvin and Kenneth Griffin Community Prevention of Alcohol Problems Harold Holder Can Screening and Brief Intervention Lead to Population-Level Reductions in Alcohol-Related Harm? Nick Heather Sharpening the Focus of Alcohol Policy from Aggregate Consumption to Harm and Risk Reduction Tim Stockwell et al A Review of the Efficacy and...

Ian Curtis (2001)

OpenAIRE

Dalwood, D

2016-01-01

This exhibition offers a presentation of FRAC Champagne-Ardenne‘s collection; artists included: Francesco Arena, Sylvie Auvray, Dara Birnbaum, Rossella Biscotti, Tom Burr, Antoine Catala, Guy de Cointet, Ann Craven, Dexter Dalwood, Hassan Darsi, Plamen Dejanoff, Marcelline Delbecq, Julien Discrit, Jimmie Durham, Ruth Ewan, Michel François, Linda Fregni Nagler, Aurélien Froment, General Idea, Oscar Giaconia, Jean-Michel Hannecart, Sharon Hayes, Charline von Heyl, Barbara Kasten, Nick Mauss, Ma...

TeleWound care – providing remote wound assessment and treatment in the home care setting: current status and future directions

OpenAIRE

Santamaria, Nick; Kapp,Suzanne

2013-01-01

Nick Santamaria,1 Suzanne Kapp2 1University of Melbourne and Melbourne Health, Royal Melbourne Hospital, Melbourne, VIC, Australia; 2Royal District Nursing Service Institute, Melbourne, VIC, Australia Abstract: The use of wound telemedicine systems in the home care environment has been expanding for the last decade. These systems can generally be grouped into two main types: store and forward systems and video conference type systems; additionally, there are also hybrid systems available tha...

TeleWound care – providing remote wound assessment and treatment in the home care setting: current status and future directions

OpenAIRE

Santamaria N; Kapp S

2013-01-01

Nick Santamaria,1 Suzanne Kapp2 1University of Melbourne and Melbourne Health, Royal Melbourne Hospital, Melbourne, VIC, Australia; 2Royal District Nursing Service Institute, Melbourne, VIC, Australia Abstract: The use of wound telemedicine systems in the home care environment has been expanding for the last decade. These systems can generally be grouped into two main types: store and forward systems and video conference type systems; additionally, there are also hybrid systems available tha...

Palmse viinaköögi manifest

Index Scriptorium Estoniae

1996-01-01

Manifest. Tõlkijate seminari puhul 17. mail 1996 Palmse viinaköögis Juta Bedia, Eric Dickens, Gyözö Fehérvári, Guntars Godinsh, Maima Grinberga, Irja Grönholm, Leif Hannibal, Cornelius Hasselblatt, Pirkko Huurto, Ivo Iliste, Nora Javorskaja, Gisbert Jänicke, Kaisu Lahikainen, Enel Melberg, Vera Ruber, Arnold Tamm, Leili Tamm, Jonatan Tomesh, Lilia Velic'kova, Marianne Vogel. Inglise k.: Estonian Literary Magazine, autumn 1996, nr. 3

Triplex configuration in the nick-free DNAs that constitute the chromosomal scaffolds in grasshopper spermatids

Czech Academy of Sciences Publication Activity Database

Černá, Adriana; Lopez-Fernandez, C.; Fernandez, J.L.; de la Espina, S.M.D.; De la Torre, C.; Gosalvez, J.

2008-01-01

Roč. 117, č. 1 (2008), s. 15-24 ISSN 0009-5915 Institutional research plan: CEZ:AV0Z50380511 Keywords : chromatid scaffold * DNA loops * triplex DNA Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.111, year: 2008

Distribuce předpon v českém sylabotónickém trocheji

Czech Academy of Sciences Publication Activity Database

Plecháč, Petr; Kolár, Robert; Hlaváčová, J.; Merthová, K.

2017-01-01

Roč. 78, č. 4 (2017), s. 322-332 ISSN 0037-7031 R&D Projects: GA ČR(CZ) GA14-31160S Institutional support: RVO:68378068 Keywords : metrics and prosody * morphology * corpus linguistics * trochee * prefixes Subject RIV: AJ - Letters, Mass-media, Audiovision OBOR OECD: Specific literatures Impact factor: 0.625, year: 2016

Uroboros : [aus "Uroboros"] : [luuletused] / Ene Mihkelson ; tlk. Irja Grönholm ja Gisbert Jänicke

Index Scriptorium Estoniae

Mihkelson, Ene, 1944-2017-

2006-01-01

Sisu: Uroboros : "In der Nacht fragt heimlich jemand Wie heisst du..." ; "Am Meer stand ein Mann mit einer Sense..." ; "An dem Tag als es Kugeln regnete..." ; "Zum Zeitvertrieb ersteigerte ich mir auf einer Auktion ein paar..." ; "Sie hat sich mit der Zeit eingelassen und daraus..." ; Das Feld ; "Mutter ist wieder bei mir obgleich..." ; "Wir waren ein Mädchen Wir redeten..." ; "Als ich vier Jahre alt wurde Da wurde so viel..." ; "Wer ist dein Vater Der Fremde aus der Ferne..." ; "Das letzte Wort Das letzte Das letzte Das allein...". Orig.: Uroboros : "Öö aegu keegi küsib sala Mis su nimi on..." ; "Mere ees seisis vikatiga mees..." ; "Sel päeval kui sadas kuulivihma..." ; "Ajaviiteks ostsin enampakkumisel sõpru..." ; "Ta ajaga läinud on sohki ja sellest..." ; Põld ; "Mu ema on jälle mu juures kuigi..." ; "Meid oli üks türduk Me rääkisime..." ; "Kui olin saanud neljaseks Nii väga kiitis..." ; "Kes on su isa Kaugelt tulnud võõras..." ; "Viimast sõna Viimast Viimast Ainu..."

Relación entre inteligencia emocional, depresión y rendimiento académico en estudiantes de psicología

Directory of Open Access Journals (Sweden)

Ubaldo Rodríguez de Alba

2012-07-01

Full Text Available Abstract This research paper is part of the funded project on emotional intelligence in the II internal meeting of University of Magdalena (Ref: II CSI-11-2010 -2012. It is a descriptive-correlational and cross-sectional design. Its main objective was to identify the relationship between Perceived Emotional Intelligence (EI, depression and academic performance in Psychology students of Santa Marta (Colombia. The study involved 76 students who applied the Beck depression inventory, the Trait Meta Mood Scale. Academic performance is registered through the academic GPA. The results indicated significant negative relationship between the emotional clarity, emotional repair and levels of depression, as well as non-existence of significant relationship between the skills of EI and the academic performance; however, the latter negatively correlate with the levels of depression. Resumen El presente artículo de investigación se enmarca en el proyecto sobre inteligencia emocional financiado en la II convocatoria interna del programa Semilleros de la Universidad del Magdalena (Ref: II CSI-11-2010-2012. Es de tipo descriptivo-correlacional y de diseño transversal. Su objetivo principal consistió en identificar la relación entre la Inteligencia Emocional Percibida (IE, depresión y rendimiento académico en estudiantes de Psicología de Santa Marta (Colombia. En el estudio participaron 76 estudiantes a los que se aplicó el inventario para la depresión de Beck, el Trait Meta Mood Scale. El rendimiento académico se registró a través de los promedios académicos ponderados. Los resultados indicaron relación negativa significativa entre la claridad emocional, la reparación emocional y los niveles de depresión, así como la no existencia de relación significativa entre las habilidades de IE y el rendimiento académico; sin embargo, este último sí se correlaciona negativamente con los niveles de depresión.

Seasonal changes in a fish assemblage associated with mangroves in a coastal lagoon of Baja California Sur, Mexico Cambios estacionales de la comunidad de peces asociada a zonas de manglar en una laguna costera de Baja California Sur, México

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Jesús Rodríguez-Romero

2011-07-01

Full Text Available The fish assemblage in a coastal lagoon with mangroves known as "Rancho Bueno" was determined and associated with environmental parameters. We used an experimental otter trawl net to catch the fish, and 62 fish species were identified from 48 genera and 30 families. The most abundant species were: Etropus crossotus, Eucinostomus gracilis, Paralabrax maculatofasciatus, Sphoeroides annulatus, and Eucinostomus dowii. The water temperature changed seasonally, being warm from July through December and cold from January through June. We found more fish species during the warm season than during the cold season. The southern area of the coastal lagoon had the highest diversity and species richness. The small size of the fishes registered confirms the ecological role of coastal lagoons as nursery areas that offer protection and feeding to commercially important fish near Bahía Magdalena, Mexico.Se determinó la estructura de peces asociada a factores ambientales en una laguna costera con manglar denominada "Rancho Bueno". Se utilizó una red de arrastre experimental para la captura y se identificaron 62 especies de peces de 48 géneros y 30 familias. Las especies más importantes fueron Etropus crossotus, Eucinostomus gracilis, Paralabrax maculatofasciatus, Sphoeroides annulatus y Eucinostomus dowii. La temperatura del agua varió estacionalmente, siendo cálida de julio a diciembre y fría de enero a junio. Se registró un mayor número de especies de peces durante la época cálida comparada con la época fría. La zona sur de la laguna costera presentó una mayor diversidad y riqueza específica. El menor tamaño de los peces registrados, confirma el papel ecológico de las lagunas costeras, consideradas como áreas de crianza las cuales proporcionan protección y alimentación a los peces de importancia comercial cerca de Bahía Magdalena, México.

SINOPSIS DE LOS ESCARABAJOS COPRÓFAGOS (COLEOPTERA: SCARABAEINAE DEL CARIBE COLOMBIANO

Directory of Open Access Journals (Sweden)

JORGE ARI NORIEGA

2013-12-01

Full Text Available La subfamilia Scarabaeinae (Coleoptera: Scarabaeidae es un grupo de gran importancia en los ecosistemas por su significativa participación en el proceso de reciclaje de nutrientes. Para Colombia existen algunos acercamientos al análisis de su diversidad en ciertas regiones biogeográficas. Sin embargo, existen zonas que necesitan un mayor estudio, como es el caso de la región Caribe. Con el fin de llenar el vacío de información se realizó una revisión de la información depositada en 16 colecciones a nivel nacional y de la literatura en esta zona. Se registran 90 especies pertenecientes a 24 géneros, agrupadas en seis tribus. Se reportan 20 nuevas especies para el departamento de La Guajira, 18 para Sucre, once para Magdalena, nueve para Bolívar, nueve para Córdoba, tres para Atlántico y dos para Cesar. El departamento con la mayor riqueza es Magdalena ya que es el mejor muestreado incluyendo su rango altitudinal. Al analizar la composición de especies, la región Caribe presenta pocos endemismos, exceptuando las zonas altas del Macizo de la Sierra Nevada de Santa Marta. El género Onthophagus es el más diverso en la región y la especie que presenta el mayor rango altitudinal es Ontherus sanctaemartae (700 hasta 2500 m . La región es homogénea en las zonas bajas, presentándose una mayor afinidad con el Chocó biogeográfico. Se establecen en la región cuatro grandes franjas altitudinales: planicies (0- 500 m , colinas altas (>500- 900 m , zona subandina-andina (> 900 a 2500 m y alta montaña (> 2500 m . Es posible que con el incremento de muestreos en zonas poco estudiadas la homogeneidad específica aumente. Finalmente, es necesario ampliar e intensificar los estudios en esta región, especialmente en los sistemas montañosos aislados.

Geology and mineral deposits of an area in the Departments of Antioquia and Caldas (Subzone IIB), Colombia

Science.gov (United States)

Feininger, Tomas; Barrero L., Dario; Castro, Nestor; Hall, R.B.

1973-01-01

The Inventario Minero National (IMN), a four-year cooperative geologic mapping and mineral resources appraisal project, was accomplished under an agreement between the Republic of Colombia and the U. S. Agency for International Development from 1964 through 1969. Subzone IIB, consisting essentially of the east half of Zone comprises nearly 20,000 km2 principally in the Department of Antioquia but including also small parts of the Departments of Caldas and Tolima. The rocks in IIB range from Precambrian to Holocene. Precambrian feldspar-quartz gneiss occupies a mosaic of fault-bounded blocks intruded by igneous rocks between the Oto fault and the Rio Magdalena. Paleozoic rocks are extensive, and include lightly metamorphosed graptolite-bearing Ordovician shale at Cristalina, and a major suite of graphitic quartz-mica schist, feldspathic and aluminous gneiss, quartzite, marble, amphibolite, and other rocks. Syntectonic intrusive gneiss included many of the older rocks during a late Paleozoic(?) orogeny, which was accompanied by Abukuma-type metamorphosing from lowermost greenschist to upper amphibolite facies. A Jurassic diorite pluton bounded by faults cuts volcanic rocks of unknown age east of the Otu fault. Cretaceous rocks are major units. Middle Cretaceous carbonaceous shale, sandstone, graywacke, conglomerate, and volcanic rocks are locally prominent. The Antioquian batholith (quartz diorite) of Late Cretaceous age cuts the middle Cretaceous and older rocks. A belt of Tertiary nonmarine clastic sedimentary rocks crops out along the Magdalena Valley. Patches of Tertiary alluvium are locally preserved in the mountains. Quaternary alluvium, much of it auriferous, is widespread in modern stream valleys. Structurally IIB constitutes part of a vast complex synclinorium intruded concordantly by syntectonic catazonal or mesozonal felsic plutons, and by the later epizonal post-tectonic Antioquian batholith. Previously unrecognized major wrench faults are outstanding

Analysis of digestive tract content of the larvae of Polybia scutellaris (White (Hymenoptera, Vespidae Análise do conteúdo do trato digestivo das larvas de Polybia scutellaris (White (Hymenoptera, Vespidae

Directory of Open Access Journals (Sweden)

Victoria Lilian Fernández Corujo

2011-06-01

Full Text Available Polybia scutellaris (White, 1841 is a social wasp of biological interest for its role as pollinator and maybe as biological control agent of sanitary and agricultural pests. This study examines the digestive tract contents of the larvae of P. scutellaris from four nests in Magdalena (Buenos Aires province, Argentina. Contents included both animal (arthropod parts and plant (pollen, leaf and fruit epidermis parts. The pollen content analysis showed that the wasps visited 19 different taxa of plants during the last active period of the colony before the nests had been collected. The range of sources used by P. scutellaris allows us characterizing the species as a generalist flower visitor. Wasps visited both native and exotic plants located nearby the nest. Most of the epidermal plant remains found in the larval digestive tract belonged to Malvaceae, a family not exploited by the studied colonies as pollen source.Polybia scutellaris (White, 1841 é uma vespa social de interesse biológico devido ao seu papel como polinizador e possível agente de controle biológico de pragas sanitárias e agrícolas. O presente estudo analisou o conteúdo do trato digestivo de larvas de P. scutellaris de quatros ninhos em Magdalena (província de Buenos Aires, Argentina. O conteúdo inclui restos animais (partes de artrópodos e vegetais (pólen, epiderme de folhas e frutos. A análise do conteúdo polínico mostrou que as vespas visitaram 19 táxons durante o último período ativo da colônia antes dos ninhos serem coletados. A diversidade de recursos utilizados por P. scutellaris permite caracterizá-la como um visitante generalista de flores. As vespas visitaram plantas nativas e exóticas localizados nas proximidades do ninho. A maioria dos restos de epiderme encontradas no trato digestivo das larvas pertence às Malvaceae, uma família não explorada pelas colônias estudadas como fonte de pólen.

La politización en las lecturas canónicas: Miguel Antonio Caro, lector de Jorge Isaacs

Directory of Open Access Journals (Sweden)

Carlos Arturo López Jiménez

2008-12-01

Full Text Available The article herein shows some conceptual features of Miguel Antonio Caro’s intellectual project and its intrinsic strengths. Furthermore, it details the type of politicization which Caro imposed on some of the work of his contemporaries politicization which, in turn, was later imposed on him-self by contemporary historiography. More to the point, I evidence how Caro, when asserting that a particular text by Jorge Isaacs (The native tribes of the Magdalena basin was scientific, immediately proceeded to politicize Isaac’s work, among other things, because he (Caro did not have the relevant arguments needed to confront Isaacs’ text in its own terms and framework. Thus, in order to dismiss Isaacs’ objections raised against the government of Nuñez, Caro places the aforementioned work by the author of María, among the texts that threatened social order by presenting it as part and parcel of positivism, empiricism, liberalism, and all other intellectual efforts based on a damaging sensualism.//Aquí muestran algunos aspectos conceptuales del proyecto intelectual de Miguel Antonio Caro y su consistencia interna. Además, preciso el tipo específico de politización que este escritor impuso sobre algunos trabajos que le fueron contemporáneos –politización de la cual el mismo Caro ha sido objeto por parte de historiografía contemporánea–. Específicamente, presento cómo Miguel Antonio Caro, al afirmar que un texto de Isaacs (Las tribus indígenas del Magdalena es científico, politiza su trabajo por la imposibilidad de atacarlo dentro de los límites argumentativos del texto de Isaacs. De esta forma, para hacer a un lado las objeciones que Isaac eleva contra el gobierno de Núñez, Caro ubica el trabajo del autor de María en el grupo de escritos que amenazan el orden social, al presentarlo de la mano con el positivismo, empirismo, liberalismo y demás esfuerzos intelectuales fundados en un “nocivo” criterio sensualista.

Algunas consideraciones geomorfológicas de la cuenca del Río de la Magdalena

Directory of Open Access Journals (Sweden)

Jorge Cervantes Borja

1969-06-01

Full Text Available En este trabajo se intenta demostrar cómo el medio físico se mantiene en una dinámica continua, sujeta tanto a acciones física como biológicas; dichas acciones reflejan fenómenos y hechos nuevos sobre el paisaje, generando una labor mixta raramente apreciada y menos aún cuantificada. La geomorfología moderna afirma que las formas actuales son el resultado de acciones pasadas, y que los procesos actuales, darán formas futuras hasta cierto punto previsibles, aunque ciertamente difíciles de cuantificar, por la complejidad de los mecanismos que entran en juego. Sobre esta base, se describe el modelado y se establece una probable evolución del mismo a partir de hechos pasados que se indican en un intento cronológico. Al mismo tiempo se analizan los procesos dinámicos que son la causa de la morfogénesis actual y esto nos permite emitir algunas recomendaciones prácticas para corregir el desequilibrio ecológico que amenaza la modificación de la cuenca.

Productive and reproductive behavior of three herd of Cebu cattle of the Magdalena Area

International Nuclear Information System (INIS)

Nossa Hernandez, M.O.; Obando Correa, H.; Castro Hernandez, A.; Gallego Marin, M.I.

1989-01-01

The productive and reproductive behavior of 3 herd of cattle is studied of Cebu cattle located in Savannah of Torres, Giron and Lebrija to the northwest of Santander, with heights of 118 to 160 m.s.n.m, temperatures of 25 to 27 centigrade degrees, relative humidity of 65 to 80 percent and plane topography with waves and acid soil. The exploitation system evolved from the continuous shepherding in native prairies to the rotational with improved prairies and mineral supplementation. The system of it mounts it was continuous and oscillate among 35 cows/bull to the beginning and 25-30 cows/bull at the end. Due to the location and different handling, the 3 herds were analyzed independently. 1, 1843 observations were analyzed to measure the interval between childbirths and 478 for the weight to the birth and the weaning (270 days). An analysis was used by minimum squares with unequal subclasses. The weight to the weaning was adjusted by covariance, due to the dispersion of the age to the weaning. For the natality rate they were processed and they analyzed for high X to the 2 3175 herds. The prevalence of infectious illnesses of the reproduction was studied in 156 blood samples and it was analyzed by the pattern 1 of Cavanzo. The intervals among childbirths for the herds 1, 2 and 3 were: 466 days (C V same 24 percent), 458 days (C V same 29 percent) and 513 days (C V same 31 percent), respectively. When studying the effect of the month of the childbirth on the interval among childbirths, the months of low precipitation (December to March and July) they diminished the interval the bull it also influenced in the duration of the interval among childbirths; in all the herds, the effect of the reproducer increased or it diminished the interval significantly. In the herd 1, the weight average to the birth was of 32 but or less 1.9 kg and the males weighed 3.8 kg more that the females. The weight to the weaning was of 219.4 but or less 16.9 kg (C V similar to 8.0 percent) and it was influenced significantly alone for the year of childbirth and for the reproducer. The distribution of the sex was lightly superior for the females (52 percent) the half rate of natality for the 3 was 78.4 percent. The herd 3 presented the rate low (74.5 percent) and the 2, the high (81.6 percent). Regarding illnesses, the 3 herd were free of brucellosis, with a very low incidence, 22 percent, of bovine viral diarrhea (DVB) and 1 parainfluenza-3 percent (PI-3) and a high prevalence (54 percent) of infectious bovine rinotraqueitis (RBI)

SECADO DE ARENCA (Triportheus magdalenae: INFLUENCIA DEL SALADO EN PILA SECA Y HÚMEDA

Directory of Open Access Journals (Sweden)

DIEGO FELIPE TIRADO ARMESTO

2017-07-01

Full Text Available The aim of this study was to determine the influence of salting on dried (SD and wet stack (WS during the drying of arenca from determining mass transfer coefficients (Kc and diffusivity (Da. Moisture content and NaCl of arenca sheets according to time was determined during salting SD and WS. Data were analyzed in the PHARM/PSC® version 4 program, and the least significant difference test (DMS was applied when a difference between the averages was observed. These data are used in a mathematical model for determining mass transfer coefficients and diffusivity. It was used for this equation concentration in function of time and position for an infinite slab of thickness 2L. It was observed that the best conditions of transfer were made for salted with SD. Depending on the type of salt no significant effects were found (p <0,05 on the transfer of water and NaCl, but whether as long as it remains in the sample solution (p <0,05.

Una vida atormentada y contradictoria: Eduardo Arias Suárez

Directory of Open Access Journals (Sweden)

Adel López Gómez

1965-07-01

Full Text Available El señor Constantino, personaje central, casi único de Bajo la luna negra, dice en uno de sus frecuentes desvaríos: "Entonces yo volví a ser yo mismo, cuando tenía. solo veinte añios. Hijo de la tierra ardiente que me tostaba la cara y me atropellaba la sangre en las arterias; cazador de jaguares en las montañas del Quindío; nadador intrépido en las aguas del Magdalena; domador de caballos y de mujeres; cantador de bambucos y as del trago, de la baraja y del cuchillo. Yo tenía mucho de la selva en las venas, y mi tierra es abrupta y áspera, con rotundidacles de abismo y de montaña".

Fish farming of native species in Colombia: current situation and perspectives

DEFF Research Database (Denmark)

Cruz-Casallas, P. E.; Medina-Robles, V. M.; Velasco-Santamaria, Y. M.

2011-01-01

. The Colombian pisciculture is based on red Tilapia Oreochromis sp. (Linnaeus), Rainbow trout Oncorhynchus mykiss (Walbaum) and cachama blanca Piaractus brachypomus (Cuvier), which currently represent around 96% of the total national production. The remaining 4% comes from other farmed species such as bocachico......In Colombia and the rest of the world, the decrease in capture fisheries production has turned the aquaculture into an alternative source of protein for the populations food security as well as an important productive activity, generating employment and income for the rural communities...... Prochilodus magdalenae (Steindachner), carp Cyprinus carpio (Linnaeus) and yamu Brycon amazonicus (Spix & Agassiz). From the three main fish species, cachama blanca is the only native species, which has shown excellent performance in pond farming due to its rusticity, omnivorous habits, docility, meat quality...

WannaBike - one of the teams that participated in Bike2Work 2017

CERN Multimedia

2017-01-01

Bike2Work is a healthy living initiative involving companies across Switzerland, and in May and June this year it inspired 54 780 participants from 1 885 companies to take to the saddle for their daily commute, while simultaneously promoting a sustainable approach to transport. In the photo one can see the team "WannaBike" from CERN. The members of the team, from left to right, were: Nick Walter, Ludivine Ceard, Cecilia Uribe Estrada and Manuel Silva.

Environmental tobacco smoke is just as damaging to DNA as mainstream smoke.

OpenAIRE

Bermúdez, E; Stone, K; Carter, K M; Pryor, W A

1994-01-01

This study demonstrates the ability of tar isolated from environmental tobacco smoke (ETS) to nick DNA in mammalian cells. Solutions of ETS tar behave similarly to aqueous solutions of cigarette tar from mainstream smoke. Both solutions contain the tar semiquinone radical, and this radical associates with the DNA in viable rat alveolar macrophages. Solutions of tar from ETS cause single-strand DNA breaks in rat thymocytes in proportion to the amount of tar present, until a plateau is reached....

Briefing paper for universities on Core Maths

OpenAIRE

Glaister, Paul

2015-01-01

This briefing paper outlines the rationale for and development of the new Core Maths qualifications, the characteristics of Core Maths, and why Core Maths is important for higher education. It is part of a communication to university vice-chancellors from the Department for Business, Innovation and Skills (BIS) comprising this paper and a joint Ministerial letter from Jo Johnson, Minister of State for Universities and Science in BIS, and Nick Gibb, Minister of State for Schools in the Departm...

Loglines. November-December 2012

Science.gov (United States)

2012-12-01

other experience and skills that are a good fit for a manager,” she said. Clay Henson, a former Army infantry- man, was one of the first service...new employees need to know that the agency provides? DLA supports warfighters. DLA provides clothing, medicine, meals , fuel and replacement parts for...first-strike ration is smaller and lighter than a meal , ready-to-eat. It is used by the U.S. Michael Tuttle and Nick Sistrun are writers for DLA

Strategic Reflections: Operation Iraqi Freedom, July 2004-February 2007

Science.gov (United States)

2012-10-01

not pan out. We knew that our mission was ultimately to hand over security responsibility to the Iraqi government, but we had not yet developed a...were “ chicken -egg” discus- sions about whether the security situation had to improve before the political track could begin. I strongly argued that...2005 Lt Gen Nick Houghton (UK), October 2005–February 2006 Lt Gen Rob Fry (UK), March 2006–September 2006 Lt Gen Graham Lamb (UK), September 2006

Psychological and social analysis of population of Goiania three years after the accident with the cesium-137

International Nuclear Information System (INIS)

Helou, Suzana; Costa Neto, Sebastiao Benicio da; Curado, Maria Paula

1995-01-01

Psychological and social analysis of population of Goiania - Brazilian city - three years after the accident with the cesium-137 occurred in 1987 are discussed. With this goal it is presented a public opinion research in order to retract the Goiania's radioactive accident residual psychological effects. The public opinion research was analyzed in sum individual answers terms, considering the factors multiplicity to what the individuals are exposed in the society. It was adopted Cabral's and Nick's concepts

Radioactive probes for human gene localisation by in situ hybridisation

International Nuclear Information System (INIS)

Fennell, S.J.

1980-07-01

Radioactive probes of high specific activity have been used for human gene localisation on metaphase chromosome preparations. Human 5S ribosomal RNA was used as a model system, as a probe for the localisation of human 5S ribosomal genes. 125 I-labelled mouse 5S ribosomal RNA was used to study the 5S ribosomal gene content and arrangement in families with translocations on the long arm of chromosome 1 close to or containing the 5S ribosomal RNA locus, by in situ hybridisation to human metaphase chromosomes from peripheral blood cultures. This confirmed the chromosomal assignment of 5S ribosomal genes to 1q 42-43. In situ hybridisation probes were also prepared from recombinant plasmids containing Xenopus laevis oocyte 5S or 28S/18S gene sequences to give [ 3 H]-labelled cRNA and [ 3 H]-labelled nick-translated plasmid DNA. Studies on the kinetics of hybridisation of plasmid probes with and without ribosomal gene sequences questioned the role of plasmid DNA for amplification of signal during gene localisation. Gene localisation was obtained with nick-translated plasmid DNA containing the 28S/18S ribosomal DNA insert after short exposure times, but poor results were obtained using a [ 3 H]-labelled cRNA probe transcribed from the plasmid with the 5S gene insert. (author)

Dual-cyclical nucleic acid strand-displacement polymerization based signal amplification system for highly sensitive determination of p53 gene.

Science.gov (United States)

Xu, Jianguo; Wu, Zai-Sheng; Li, Hongling; Wang, Zhenmeng; Le, Jingqing; Zheng, Tingting; Jia, Lee

2016-12-15

In the present study, we proposed a novel dual-cyclical nucleic acid strand-displacement polymerization (dual-CNDP) based signal amplification system for highly sensitive determination of tumor suppressor genes. The system primarily consisted of a signaling hairpin probe (SHP), a label-free hairpin probe (LHP) and an initiating primer (IP). The presence of target DNA was able to induce one CNDP through continuous process of ligation, polymerization and nicking, leading to extensively accumulation of two nicked triggers (NT1 and NT2). Intriguingly, the NT1 could directly hybridize SHP, while the NT2 could act as the target analog to induce another CNDP. The resulting dual-CNDP contributed the striking signal amplification, and only a very weak blank noise existed since the ligation template of target was not involved. In this case, the target could be detected in a wide linear range (5 orders of magnitude), and a low detection limit (78 fM) was obtained, which is superior to most of the existing fluorescent methods. Moreover, the dual-CNDP sensing system provided a high selectivity towards target DNA against mismatched target and was successfully applied to analysis of target gene extracted from cancer cells or in human serum-contained samples, indicating its great potential for practical applications. Copyright © 2016 Elsevier B.V. All rights reserved.

Palindromic Molecule Beacon-Based Cascade Amplification for Colorimetric Detection of Cancer Genes.

Science.gov (United States)

Shen, Zhi-Fa; Li, Feng; Jiang, Yi-Fan; Chen, Chang; Xu, Huo; Li, Cong-Cong; Yang, Zhe; Wu, Zai-Sheng

2018-03-06

A highly sensitive and selective colorimetric assay based on a multifunctional molecular beacon with palindromic tail (PMB) was proposed for the detection of target p53 gene. The PMB probe can serve as recognition element, primer, and polymerization template and contains a nicking site and a C-rich region complementary to a DNAzyme. In the presence of target DNA, the hairpin of PMB is opened, and the released palindromic tails intermolecularly hybridize with each other, triggering the autonomous polymerization/nicking/displacement cycles. Although only one type of probe is involved, the system can execute triple and continuous polymerization strand displacement amplifications, generating large amounts of G-quadruplex fragments. These G-rich fragments can bind to hemin and form the DNAzymes that possess the catalytic activity similar to horseradish peroxidase, catalyzing the oxidation of ABTS by H 2 O 2 and producing the colorimetric signal. Utilizing the newly proposed sensing system, target DNA can be detected down to 10 pM with a linear response range from 10 pM to 200 nM, and mutant target DNAs are able to be distinguished even by the naked eye. The desirable detection sensitivity, high specificity, and operation convenience without any separation step and chemical modification demonstrate that the palindromic molecular beacon holds the potential for detecting and monitoring a variety of nucleic acid-related biomarkers.

Genomic Mapping of Human DNA provides Evidence of Difference in Stretch between AT and GC rich regions

Science.gov (United States)

Reifenberger, Jeffrey; Dorfman, Kevin; Cao, Han

Human DNA is a not a polymer consisting of a uniform distribution of all 4 nucleic acids, but rather contains regions of high AT and high GC content. When confined, these regions could have different stretch due to the extra hydrogen bond present in the GC basepair. To measure this potential difference, human genomic DNA was nicked with NtBspQI, labeled with a cy3 like fluorophore at the nick site, stained with YOYO, loaded into a device containing an array of nanochannels, and imaged. Over 473,000 individual molecules of DNA, corresponding to roughly 30x coverage of a human genome, were collected and aligned to the human reference. Based on the known AT/GC content between aligned pairs of labels, the stretch was measured for regions of similar size but different AT/GC content. We found that regions of high GC content were consistently more stretched than regions of high AT content between pairs of labels varying in size between 2.5 kbp and 500 kbp. We measured that for every 1% increase in GC content there was roughly a 0.06% increase in stretch. While this effect is small, it is important to take into account differences in stretch between AT and GC rich regions to improve the sensitivity of detection of structural variations from genomic variations. NIH Grant: R01-HG006851.

Human DNA ligase III bridges two DNA ends to promote specific intermolecular DNA end joining

Science.gov (United States)

Kukshal, Vandna; Kim, In-Kwon; Hura, Gregory L.; Tomkinson, Alan E.; Tainer, John A.; Ellenberger, Tom

2015-01-01

Mammalian DNA ligase III (LigIII) functions in both nuclear and mitochondrial DNA metabolism. In the nucleus, LigIII has functional redundancy with DNA ligase I whereas LigIII is the only mitochondrial DNA ligase and is essential for the survival of cells dependent upon oxidative respiration. The unique LigIII zinc finger (ZnF) domain is not required for catalytic activity but senses DNA strand breaks and stimulates intermolecular ligation of two DNAs by an unknown mechanism. Consistent with this activity, LigIII acts in an alternative pathway of DNA double strand break repair that buttresses canonical non-homologous end joining (NHEJ) and is manifest in NHEJ-defective cancer cells, but how LigIII acts in joining intermolecular DNA ends versus nick ligation is unclear. To investigate how LigIII efficiently joins two DNAs, we developed a real-time, fluorescence-based assay of DNA bridging suitable for high-throughput screening. On a nicked duplex DNA substrate, the results reveal binding competition between the ZnF and the oligonucleotide/oligosaccharide-binding domain, one of three domains constituting the LigIII catalytic core. In contrast, these domains collaborate and are essential for formation of a DNA-bridging intermediate by adenylated LigIII that positions a pair of blunt-ended duplex DNAs for efficient and specific intermolecular ligation. PMID:26130724

Control design profits heliostat techno-economics

CSIR Research Space (South Africa)

Rubin, N

2012-05-01

Full Text Available , and the economic impacts associated with reduced spillage at the receiver, that accrue through improved analysis, modelling and design techniques. 2. Background 2.1. Previous Results It is testimony to the robustness of feedback control that a stabilizing... Heliostat Techno-Economics Nick Rubin1, Thomas Roos2, Fintan Wilson2, Jason de Villiers2 and Strinivasan Perumal2 1 Systems Engineer ? CSIR, P O Box 395, Pretoria, 0001, South Africa, Phone: 0128413383, Fax:0128413179, NRubin@csir.co.za 2 THRoos...

Violenica y trabajadores del petroleo

Directory of Open Access Journals (Sweden)

Renan Vega Cantor

2009-12-01

Full Text Available In This article shows the research results which aims at proving the dramatic situation of a region in Colombia which has become one of the war scenarios, on the part of paramilitary groups, against leaders, social activists or political militants or dissidents. For the last 25 years, there have been a hundred killings or so of union workers linked with Worker's Union (USO and in order to understand this phenomenon, it is necessary to define the historic context and point out how Paramilitarism appeared and expanded throughout Magdalena Medio at the beginning of the 1980's, which was financed and sponsored by different sectors of the Colombian society. It is necessary to highlight that the attack against union workers is a crime against humanity, both in Colombia and the rest of the world.

Evaluando el estado de la biodiversidad: el caso de la avifauna de la Serranía de las Quinchas, Boyacá, Colombia

Directory of Open Access Journals (Sweden)

Stiles Hurd Frank Galfierd

2000-07-01

Full Text Available Using sight and auditory records made during extensive observations, supplemented by mist-net captures and tape recordings, we made an inventory of the birds of three sites differing in elevation and degree of human transformation of the landscape, in the Serranía de las Quinchas in the middle Magdalena valley of Colombia. We estimated per cent cover of different habitat types in each site and abundances of birds in each, based upon encounter frequencies, and developed an ecological classification of the birds that emphasized their degrees of association with primary, disturbed or manmade habitats. With 10-1 3 field days per site and a total of 833 birds captured in 1083 net-hours, we recorded a total of 308 species in the studv area. The form of the species accumulation curves and distributions of abundances indicated that the samples from the three sites were comparable. The numbers and abundances of species in different ecological categories varied in accord with the representation of the different habitat types between sites; the two sites with the largest extensions of primary forest had greater species richness. The majority of forest-restricted species occurred at a single site; on average, the most generalized species with respect to tree cover occured in two or more sites; and species of open habitats occurred in one site (elevation specialists or all three. Most species were breeding in April, also the best month for auditory records. The suboscine passerines of the suborder Furnarii (antbirds, ovenbirds and allies include the largest single group of forest- restricted species which could serve as an indicator of the state of conservation of forest habitat, provided the appropriate species are chosen - most families include species with diverse habitat requirements. The scarcity or absence of several groups subject to hunting pressure was noteworthy in an otherwise healthy forest avifauna. The known or suspected presence of several

LA VEGETACIÓN TERRESTRE EN LA ENSENADA DE NEGUANJE, PARQUE NACIONAL NATURAL TAYRONA (MAGDALENA, COLOMBIA

Directory of Open Access Journals (Sweden)

Carbonó Eduino

2010-12-01

Full Text Available Entre los meses de julio y diciembre de 2007 se realizaron inventarios que permitieronconocer el estado actual de la riqueza y la estructura de la vegetación en la ensenadade Neguanje, Parque Nacional Natural Tayrona. Se siguió el método propuesto por elInstituto Humboldt para muestreo de plantas leñosas en tres sitios, para un total de 0.3ha. La evaluación realizada ofrece como resultado el registro de 1310 individuos conDAP ≥ 2.5 cm, correspondientes a 77 especies, de 28 familias de plantas con fl ores.Para cada uno de los tres sitios muestreados, se encontró un total de 29 especies (15familias, 49 (21 familias y 55 (24 familias por 1000 m². Las familias presentes,con mayor valor ecológico, son comunes en la mayoría de los bosques secos deColombia, sin embargo, es notoria la relativa baja diversidad específi ca, si se comparacon evaluaciones ejecutadas en ambientes semejantes. Se destaca la acción de losvientos intensos y persistentes como un factor infl uyente sobre la diversidad fl orísticaen el primer sitio estudiado.

Early results of micro-deformation measurements in Magdalena Jama (Slovenia) by a vertical static pendulum

Czech Academy of Sciences Publication Activity Database

Kalenda, Pavel; Neumann, L.; Šebela, S.

2013-01-01

Roč. 42, č. 1 (2013), s. 143-154 ISSN 0583-6050 Institutional support: RVO:67985891 Keywords : tilt * pendulum * deformations * earthquakes Subject RIV: JL - Materials Fatigue, Friction Mechanics Impact factor: 0.710, year: 2013 http://ojs.zrc-sazu.si/carsologica/article/view/635

La zona bananera del magdalena, del ensueño de la multinacional a la realidad

Directory of Open Access Journals (Sweden)

Alvaro Mercado Suarez

2014-02-01

Full Text Available Con la expedicion de la ley 136 de 1994  se esblece un nuevo reimen administrativo municipal que flexibiliza los requisitos en materia de poblacion y recursos para crear ante municipales  y la ley 134 de 1994.