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Sample records for naked mole-rat heterocephalus

  1. Structural Features of the Telomerase RNA Gene in the Naked Mole Rat Heterocephalus glaber

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    Evfratov, S. A.; Smekalova, E. M.; Golovin, A. V.; Logvina, N. A.; Zvereva, M. I.; Dontsova, O. A.

    2014-01-01

    Telomere length, an important feature of life span control, is dependent on the activity of telomerase (a key enzyme of the telomere-length-maintaining system). Telomerase RNA is a component of telomerase and, thus, is crucial for its activity. The structures of telomerase RNA genes and their promoter regions were compared for the long-living naked mole rat and different organisms. Two rare polymorphisms in Heterocephalus glaber telomerase RNA (hgTER) were identified: A→G in the first loop of pseudoknot P2b-p3 (an equivalent of 111nt in hTR) and G→A in the scaRNA domain CR7-p8b (an equivalent of 421nt in hTR). Analysis of TER promoter regions allowed us to identify two new transcription factor binding sites. The first one is the ETS family site, which was found to be a conserved element for all the analyzed TER promoters. The second site is unique for the promoter region of TER of the naked mole rat and is a binding site for the SOX17 transcription factor. The absence of one Sp1 site in the TER promoter region of the naked small rat is an additional specific feature of the promoter area of hgTER. Such variation in the hgTER transcription regulation region and hgTER itself could provide increased telomerase activity in stem cells and an extended lifespan to H. glaber. PMID:25093110

  2. Cutaneous and periodontal inputs to the cerebellum of the naked mole-rat (Heterocephalus glaber

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    Diana K Sarko

    2013-11-01

    Full Text Available The naked mole-rat (Heterocephalus glaber is a small fossorial rodent with specialized dentition that is reflected by the large cortical area dedicated to representation of the prominent incisors. Due to naked mole-rats’ behavioral reliance on the incisors for digging and for manipulating objects, as well as their ability to move the lower incisors independently, we hypothesized that expanded somatosensory representations of the incisors would be present within the cerebellum in order to accommodate a greater degree of proprioceptive, cutaneous, and periodontal input. Multiunit electrophysiological recordings targeting the ansiform lobule were used to investigate tactile inputs from receptive fields on the entire body with a focus on the incisors. Similar to other rodents, a fractured somatotopy appeared to be present with discrete representations of the same receptive fields repeated within each folium of the cerebellum. These findings confirm the presence of somatosensory inputs to a large area of the naked mole-rat cerebellum with particularly extensive representations of the lower incisors and mystacial vibrissae. We speculate that these extensive inputs facilitate processing of tactile cues as part of a sensorimotor integration network that optimizes how sensory stimuli are acquired through active exploration and in turn adjusts motor outputs (such as independent movement of the lower incisors. These results highlight the diverse sensory specializations and corresponding brain organizational schemes that have evolved in different mammals to facilitate exploration of and interaction with their environment.

  3. Peripheral administration of oxytocin increases social affiliation in the naked mole-rat (Heterocephalus glaber).

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    Mooney, Skyler J; Douglas, Natasha R; Holmes, Melissa M

    2014-04-01

    The neuropeptide oxytocin regulates a wide variety of social behaviors across diverse species. However, the types of behaviors that are influenced by this hormone are constrained by the species in question and the social organization that a particular species exhibits. Therefore, the present experiments investigated behaviors regulated by oxytocin in a eusocial mammalian species by using the naked mole-rat (Heterocephalus glaber). In Experiment 1, adult non-breeding mole-rats were given intraperitoneal injections of either oxytocin (1mg/kg or 10mg/kg) or saline on alternate days. Animals were then returned to their colony and behavior was recorded for minutes 15-30 post-injection. Both doses of oxytocin increased huddling behavior during this time period. In Experiment 2, animals received intraperitoneal injections of either oxytocin (1mg/kg), an oxytocin-receptor antagonist (0.1mg/kg), a cocktail of oxytocin and the antagonist, or saline across 4 testing days in a counterbalanced design. Animals were placed in either a 2-chamber arena with a familiar conspecific or in a small chamber with 1week old pups from their home colony and behaviors were recorded for minutes 15-30 post-injection. Oxytocin increased investigation of, and time spent in close proximity to, a familiar conspecific; these effects were blocked by the oxytocin antagonist. No effects were seen on pup-directed behavior. These data suggest that oxytocin is capable of modulating affiliative-like behavior in this eusocial species.

  4. Selective inflammatory pain insensitivity in the African naked mole-rat (Heterocephalus glaber.

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    Thomas J Park

    2008-01-01

    Full Text Available In all mammals, tissue inflammation leads to pain and behavioral sensitization to thermal and mechanical stimuli called hyperalgesia. We studied pain mechanisms in the African naked mole-rat, an unusual rodent species that lacks pain-related neuropeptides (e.g., substance P in cutaneous sensory fibers. Naked mole-rats show a unique and remarkable lack of pain-related behaviors to two potent algogens, acid and capsaicin. Furthermore, when exposed to inflammatory insults or known mediators, naked mole-rats do not display thermal hyperalgesia. In contrast, naked mole-rats do display nocifensive behaviors in the formalin test and show mechanical hyperalgesia after inflammation. Using electrophysiology, we showed that primary afferent nociceptors in naked mole-rats are insensitive to acid stimuli, consistent with the animal's lack of acid-induced behavior. Acid transduction by sensory neurons is observed in birds, amphibians, and fish, which suggests that this tranduction mechanism has been selectively disabled in the naked mole-rat in the course of its evolution. In contrast, nociceptors do respond vigorously to capsaicin, and we also show that sensory neurons express a transient receptor potential vanilloid channel-1 ion channel that is capsaicin sensitive. Nevertheless, the activation of capsaicin-sensitive sensory neurons in naked mole-rats does not produce pain-related behavior. We show that capsaicin-sensitive nociceptors in the naked mole-rat are functionally connected to superficial dorsal horn neurons as in mice. However, the same nociceptors are also functionally connected to deep dorsal horn neurons, a connectivity that is rare in mice. The pain biology of the naked mole-rat is unique among mammals, thus the study of pain mechanisms in this unusual species can provide major insights into what constitutes "normal" mammalian nociception.

  5. Expression pattern of cadherins in the naked mole rat (Heterocephalus glaber) suggests innate cortical diversification of the cerebrum.

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    Matsunaga, Eiji; Nambu, Sanae; Iriki, Atsushi; Okanoya, Kazuo

    2011-06-15

    The cerebral cortex is an indispensable region for higher cognitive function that is remarkably diverse among mammalian species. Although previous research has shown that the cortical area map in the mammalian cerebral cortex is formed by innate and activity-dependent mechanisms, it remains unknown how these mechanisms contribute to the evolution and diversification of the functional cortical areas in various species. The naked mole rat (Heterocephalus glaber) is a subterranean, eusocial rodent. Physiological and anatomical studies have revealed that the visual system is regressed and the somatosensory system is enlarged. To examine whether species differences in cortical area development are caused by intrinsic factors or environmental factors, we performed comparative gene expression analysis of neonatal naked mole rat and mouse brains. The expression domain of cadherin-6, a somatosensory marker, was expanded caudally and shifted dorsally in the cortex, whereas the expression domain of cadherin-8, a visual marker, was reduced caudally in the neonatal naked mole rat cortex. The expression domain of cadherin-8 was also reduced in other visual areas, such as the lateral geniculate nucleus and superior colliculus. Immunohistochemical analysis of thalamocortical fibers further suggested that somatosensory input did not affect cortical gene expression in the neonatal naked mole rat brain. These results suggest that the development of the somatosensory system and the regression of the visual system in the naked mole rat cortex are due to intrinsic genetic mechanisms as well as sensory input-dependent mechanisms. Intrinsic genetic mechanisms thus appear to contribute to species diversity in cortical area formation. Copyright © 2011 Wiley-Liss, Inc.

  6. Novel husbandry techniques support survival of naked mole rat (Heterocephalus glaber) pups.

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    Ke, Zhonghe; Vaidya, Amita; Ascher, Jill; Seluanov, Andrei; Gorbunova, Vera

    2014-01-01

    The naked mole rat (NMR) is a small eusocial rodent. Because of its remarkable longevity (maximal lifespan, 32 y) and resistance to cancer, the NMR has emerged as a valuable model for aging and cancer research. However, breeding NMR can be difficult. Here, we report the successful introduction and acceptance of pups into a foreign colony with existing pups of different ages. Among the 7 NMR colonies in our satellite facility, one had a consistently poor record of pup viability, with nearly 100% preweaning mortality in multiple litters born over the course of 2 y. The queen of this colony gave birth to 18 pups in January 2013; by 2 d after parturition, it was evident that the pups were not receiving sufficient nourishment. To salvage the litter, the most vigorous pups were cross-fostered to another queen that had recently given birth. On postparturition day 1 (PD1), two pups from the poorly nourished donor litter were bathed with warm water, rolled in recipient colony bedding, and transferred to the recipient colony, which included 8 PD14 pups. The new pups were accepted by the recipient queen, who continued to produce milk in response to suckling by the donor pups well past the weaning of her own litter. This case report provides evidence of successful cross-fostering of NMR pups despite age differences between donor pups and those in the recipient litter; this technique may prove beneficial to researchers struggling with NMR breeding issues.

  7. Investigation of the presence and antinociceptive function of muscarinic acetylcholine receptors in the African naked mole-rat (Heterocephalus glaber)

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    Jørgensen, Kristine B.; Krogh-Jensen, Karen; Pickering, Darryl S;

    2016-01-01

    musculus) using basic local alignment search tool (BLAST). The presence and function of M1 and M4 was investigated in vivo, using the formalin test with the muscarinic receptor agonists xanomeline and VU0152100. Spinal cord tissue from the naked mole-rat was used for receptor saturation binding studies...... with [3H]-N-methylscopolamine. The BLAST test revealed 95 % protein sequence homology showing the naked mole-rat to have the genetic potential to express all five muscarinic acetylcholine receptor subtypes. A significant reduction in pain behavior was demonstrated after administration of 8.4 mg...

  8. The effects of oxotremorine, epibatidine, atropine, mecamylamine and naloxone in the tail-flick, hot-plate, and formalin tests in the naked mole-rat (Heterocephalus glaber)

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    Dulu, Thomas D; Kanui, Titus I; Towett, Philemon K

    2014-01-01

    -plate, and the formalin tests. The effects of co-administration of the muscarinic receptor antagonist atropine, the nicotinic receptor antagonist mecamylamine, and the opioid receptor antagonist naloxone were also investigated. Oxotremorine and epibatidine induced a significant, dose-dependent antinociceptive effect...... in the tail-flick, hot-plate, and formalin tests, respectively. The effects of oxotremorine and epibatidine were blocked by atropine and mecamylamine, respectively. In all three nociceptive tests, naloxone in combination with oxotremorine or epibatidine enhanced the antinociceptive effects of the drugs....... The present study demonstrated that stimulation of muscarinic and nicotinic receptors produces antinociceptive effects in the naked-mole rat. The reversal effect of atropine and mecamylamine suggests that this effect is mediated by cholinergic receptors. As naloxone increases the antinociceptive effects...

  9. Genome sequencing reveals insights into physiology and longevity of the naked mole rat

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    Kim, Eun Bae; Fang, Xiaodong; Fushan, Alexey A

    2011-01-01

    The naked mole rat (Heterocephalus glaber) is a strictly subterranean, extraordinarily long-lived eusocial mammal. Although it is the size of a mouse, its maximum lifespan exceeds 30 years, making this animal the longest-living rodent. Naked mole rats show negligible senescence, no age......-related increase in mortality, and high fecundity until death. In addition to delayed ageing, they are resistant to both spontaneous cancer and experimentally induced tumorigenesis. Naked mole rats pose a challenge to the theories that link ageing, cancer and redox homeostasis. Although characterized...... by significant oxidative stress, the naked mole rat proteome does not show age-related susceptibility to oxidative damage or increased ubiquitination. Naked mole rats naturally reside in large colonies with a single breeding female, the 'queen', who suppresses the sexual maturity of her subordinates. They also...

  10. High-molecular-mass hyaluronan mediates the cancer resistance of the naked mole rat.

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    Tian, Xiao; Azpurua, Jorge; Hine, Christopher; Vaidya, Amita; Myakishev-Rempel, Max; Ablaeva, Julia; Mao, Zhiyong; Nevo, Eviatar; Gorbunova, Vera; Seluanov, Andrei

    2013-07-18

    The naked mole rat (Heterocephalus glaber) displays exceptional longevity, with a maximum lifespan exceeding 30 years. This is the longest reported lifespan for a rodent species and is especially striking considering the small body mass of the naked mole rat. In comparison, a similarly sized house mouse has a maximum lifespan of 4 years. In addition to their longevity, naked mole rats show an unusual resistance to cancer. Multi-year observations of large naked mole-rat colonies did not detect a single incidence of cancer. Here we identify a mechanism responsible for the naked mole rat's cancer resistance. We found that naked mole-rat fibroblasts secrete extremely high-molecular-mass hyaluronan (HA), which is over five times larger than human or mouse HA. This high-molecular-mass HA accumulates abundantly in naked mole-rat tissues owing to the decreased activity of HA-degrading enzymes and a unique sequence of hyaluronan synthase 2 (HAS2). Furthermore, the naked mole-rat cells are more sensitive to HA signalling, as they have a higher affinity to HA compared with mouse or human cells. Perturbation of the signalling pathways sufficient for malignant transformation of mouse fibroblasts fails to transform naked mole-rat cells. However, once high-molecular-mass HA is removed by either knocking down HAS2 or overexpressing the HA-degrading enzyme, HYAL2, naked mole-rat cells become susceptible to malignant transformation and readily form tumours in mice. We speculate that naked mole rats have evolved a higher concentration of HA in the skin to provide skin elasticity needed for life in underground tunnels. This trait may have then been co-opted to provide cancer resistance and longevity to this species.

  11. The Naked Mole Rat Genome Resource: facilitating analyses of cancer and longevity-related adaptations

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    Keane, Michael; Craig, Thomas; Alföldi, Jessica; Berlin, Aaron M.; Johnson, Jeremy; Seluanov, Andrei; Gorbunova, Vera; Di Palma, Federica; Lindblad-Toh, Kerstin; Church, George M.; de Magalhães, João Pedro

    2014-01-01

    Motivation: The naked mole rat (Heterocephalus glaber) is an exceptionally long-lived and cancer-resistant rodent native to East Africa. Although its genome was previously sequenced, here we report a new assembly sequenced by us with substantially higher N50 values for scaffolds and contigs. Results: We analyzed the annotation of this new improved assembly and identified candidate genomic adaptations which may have contributed to the evolution of the naked mole rat’s extraordinary traits, including in regions of p53, and the hyaluronan receptors CD44 and HMMR (RHAMM). Furthermore, we developed a freely available web portal, the Naked Mole Rat Genome Resource (http://www.naked-mole-rat.org), featuring the data and results of our analysis, to assist researchers interested in the genome and genes of the naked mole rat, and also to facilitate further studies on this fascinating species. Availability and implementation: The Naked Mole Rat Genome Resource is freely available online at http://www.naked-mole-rat.org. This resource is open source and the source code is available at https://github.com/maglab/naked-mole-rat-portal. Contact: jp@senescence.info PMID:25172923

  12. Absence of histamine-induced itch in the African naked mole-rat and "rescue" by Substance P

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    Lewin Gary R

    2010-05-01

    Full Text Available Abstract Recent research has proposed a pathway in which sensory neurons expressing the capsaicin activated ion channel TRPV1 are required for histamine-induced itch and subsequent scratching behavior. We examined histamine-induced itch in the African naked mole-rat (Heterocephalus glaber and found that although naked mole-rats display innate scratching behavior, histamine was unable to evoke increased scratching as is observed in most mouse strains. Using calcium imaging, we examined the histamine sensitivity of naked mole-rat dorsal root ganglia (DRG neurons and identified a population of small diameter neurons activated by histamine, the majority of which are also capsaicin-sensitive. This suggested that naked mole-rat sensory neurons are activated by histamine, but that spinal dorsal horn processing of sensory information is not the same as in other rodents. We have previously shown that naked mole-rats naturally lack substance P (SP in cutaneous C-fibers, but that the neurokinin-1 receptor is expressed in the superficial spinal cord. This led us to investigate if SP deficiency plays a role in the lack of histamine-induced scratching in this species. After intrathecal administration of SP into the spinal cord we observed robust scratching behavior in response to histamine injection. Our data therefore support a model in which TRPV1-expressing sensory neurons are important for histamine-induced itch. In addition, we demonstrate a requirement for active, SP-induced post-synaptic drive to enable histamine sensitive afferents to drive itch-related behavior in the naked mole-rat. These results illustrate that it is altered dorsal horn connectivity of nociceptors that underlies the lack of itch and pain-related behavior in the naked mole-rat.

  13. RNA sequencing reveals differential expression of mitochondrial and oxidation reduction genes in the long-lived naked mole-rat when compared to mice.

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    Chuanfei Yu

    Full Text Available The naked mole-rat (Heterocephalus glaber is a long-lived, cancer resistant rodent and there is a great interest in identifying the adaptations responsible for these and other of its unique traits. We employed RNA sequencing to compare liver gene expression profiles between naked mole-rats and wild-derived mice. Our results indicate that genes associated with oxidoreduction and mitochondria were expressed at higher relative levels in naked mole-rats. The largest effect is nearly 300-fold higher expression of epithelial cell adhesion molecule (Epcam, a tumour-associated protein. Also of interest are the protease inhibitor, alpha2-macroglobulin (A2m, and the mitochondrial complex II subunit Sdhc, both ageing-related genes found strongly over-expressed in the naked mole-rat. These results hint at possible candidates for specifying species differences in ageing and cancer, and in particular suggest complex alterations in mitochondrial and oxidation reduction pathways in the naked mole-rat. Our differential gene expression analysis obviated the need for a reference naked mole-rat genome by employing a combination of Illumina/Solexa and 454 platforms for transcriptome sequencing and assembling transcriptome contigs of the non-sequenced species. Overall, our work provides new research foci and methods for studying the naked mole-rat's fascinating characteristics.

  14. Naked mole-rat has increased translational fidelity compared with the mouse, as well as a unique 28S ribosomal RNA cleavage

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    Azpurua, Jorge; Ke, Zhonghe; Chen, Iris X.; Zhang, Quanwei; Ermolenko, Dmitri N.; Zhang, Zhengdong D.; Gorbunova, Vera; Seluanov, Andrei

    2013-01-01

    The naked mole-rat (Heterocephalus glaber) is a subterranean eusocial rodent with a markedly long lifespan and resistance to tumorigenesis. Multiple data implicate modulation of protein translation in longevity. Here we report that 28S ribosomal RNA (rRNA) of the naked mole-rat is processed into two smaller fragments of unequal size. The two breakpoints are located in the 28S rRNA divergent region 6 and excise a fragment of 263 nt. The excised fragment is unique to the naked mole-rat rRNA and does not show homology to other genomic regions. Because this hidden break site could alter ribosome structure, we investigated whether translation rate and amino acid incorporation fidelity were altered. We report that naked mole-rat fibroblasts have significantly increased translational fidelity despite having comparable translation rates with mouse fibroblasts. Although we cannot directly test whether the unique 28S rRNA structure contributes to the increased fidelity of translation, we speculate that it may change the folding or dynamics of the large ribosomal subunit, altering the rate of GTP hydrolysis and/or interaction of the large subunit with tRNA during accommodation, thus affecting the fidelity of protein synthesis. In summary, our results show that naked mole-rat cells produce fewer aberrant proteins, supporting the hypothesis that the more stable proteome of the naked mole-rat contributes to its longevity. PMID:24082110

  15. High molecular weight hyaluronan mediates the cancer resistance of the naked mole-rat

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    Tian, Xiao; Azpurua, Jorge; Hine, Christopher; Vaidya, Amita; Myakishev-Rempel, Max; Ablaeva, Julia; Mao, Zhiyong; Nevo, Eviatar; Gorbunova, Vera; Seluanov, Andrei

    2013-01-01

    The naked mole-rat displays exceptional longevity, with a maximum lifespan exceeding 30 years1–3. This is the longest reported lifespan for a rodent species and is especially striking considering the small body mass of the naked mole-rat. In comparison, a similarly sized house mouse has a maximum lifespan of 4 years4,5. In addition to their longevity, naked mole-rats show an unusual resistance to cancer. Multi-year observations of large naked mole-rat colonies did not detect a single incidence of cancer2,6. Here we identify a mechanism responsible for the naked mole-rat’s cancer resistance. We found that naked mole-rat fibroblasts secrete extremely high molecular weight hyaluronan (HA), which is over five times larger than human or mouse HA. This high molecular weight HA accumulates abundantly in naked mole rat tissues due to the decreased activity of HA-degrading enzymes and a unique sequence of hyaluronan synthase 2 (HAS2). Furthermore, the naked mole-rat cells are more sensitive to HA signaling, as the naked mole rat cells have a higher affinity to HA than the mouse or human cells. Perturbation of the signaling pathways sufficient for malignant transformation of mouse fibroblasts fails to transform naked mole-rat cells. However, once high molecular weight HA is removed by either knocking down HAS2 or overexpressing the HA-degrading enzyme, Hyal2, naked mole-rat cells become susceptible to malignant transformation and readily form tumors in mice. We speculate that naked mole-rats have evolved a higher concentration of HA in the skin to provide skin elasticity needed for life in underground tunnels. This trait may have then been co-opted to provide cancer resistance and longevity to this species. PMID:23783513

  16. Assessment of naked mole-rat distribution and threats in Eastern Ethiopia

    Institute of Scientific and Technical Information of China (English)

    Mengistu Wale; Abeje Kassie; Tesfu Fekensa

    2016-01-01

    Objective:To identify the distribution, threats and community attitudes towards naked mole-rat in Eastern Ethiopia. Methods: Data were collected through direct observation and interview andChi-square at 95% confidence interval was used for significance test. Results:Naked mole-rat was identified in Fafan, City/Shinele,Eastern Hararghe Zone and Dire Dawa Administrative. The main threats of naked mole-rat identified were agricultural expansion, human killing and lack of awareness. Froma total of 100 respondents, 92% of them considered naked mole-rat as pest as a result that 46% of them participated in direct killing. Literacy rate significantly affects thewillingness to participate in the conservation of naked mole-rat (χ2 = 7.478,df = 1,P Conclusions:Naked mole-rat is fairly common in many of the study sites. However, rapid shift from nomadic life style to cultivation of crops and lacks of awareness were the main threats of naked mole-rat. Therefore, since there is no conservation action currently, further comprehensive study is required to design conservation plan for this species.

  17. Hypersensitivity to contact inhibition provides a clue to cancer resistance of naked mole-rat

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    Seluanov, Andrei; Hine, Christopher; Azpurua, Jorge; Feigenson, Marina; Bozzella, Michael; Mao, Zhiyong; Catania, Kenneth C.; Gorbunova, Vera

    2009-01-01

    The naked mole-rat is the longest living rodent with a maximum lifespan exceeding 28 years. In addition to its longevity, naked mole-rats have an extraordinary resistance to cancer as tumors have never been observed in these rodents. Furthermore, we show that a combination of activated Ras and SV40 LT fails to induce robust anchorage-independent growth in naked mole-rat cells, while it readily transforms mouse fibroblasts. The mechanisms responsible for the cancer resistance of naked mole-rats were unknown. Here we show that naked mole-rat fibroblasts display hypersensitivity to contact inhibition, a phenomenon we termed “early contact inhibition.” Contact inhibition is a key anticancer mechanism that arrests cell division when cells reach a high density. In cell culture, naked mole-rat fibroblasts arrest at a much lower density than those from a mouse. We demonstrate that early contact inhibition requires the activity of p53 and pRb tumor suppressor pathways. Inactivation of both p53 and pRb attenuates early contact inhibition. Contact inhibition in human and mouse is triggered by the induction of p27Kip1. In contrast, early contact inhibition in naked mole-rat is associated with the induction of p16Ink4a. Furthermore, we show that the roles of p16Ink4a and p27Kip1 in the control of contact inhibition became temporally separated in this species: the early contact inhibition is controlled by p16Ink4a, and regular contact inhibition is controlled by p27Kip1. We propose that the additional layer of protection conferred by two-tiered contact inhibition contributes to the remarkable tumor resistance of the naked mole-rat. PMID:19858485

  18. High Autophagy in the Naked Mole Rat may Play a Significant Role in Maintaining Good Health

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    Shanmin Zhao

    2014-02-01

    Full Text Available Background/Aims: The maximum lifespan of the naked mole rat is over 28.3 years, which exceeds that of any other rodent species, suggesting that age-related changes in its body composition and functionality are either attenuated or delayed in this extraordinarily long-lived species. However, the mechanisms underlying the aging process in this species are poorly understood. In this study, we investigated whether long-lived naked mole rats display more autophagic activity than short-lived mice. Methods: Hepatic stellate cells isolated from naked mole rats were treated with 50 nM rapamycin or 20 mM 3-methyladenine (3-MA for 12 or 24 h. Expression of the autophagy marker proteins LC3-II and beclin 1 was measured with western blotting and immunohistochemistry. The induction of apoptosis was analyzed by flow cytometry. Results: Our results demonstrate that one-day-old naked mole rats have higher levels of autophagy than one-day-old short-lived C57BL/6 mice, and that both adult naked mole rats (eight months old and adult C57BL/6 mice (eight weeks old have high basal levels of autophagy, which may be an important mechanism inhibiting aging and reducing the risk of age-related diseases. Conclusion: Here, we report that autophagy facilitated the survival of hepatic stellate cells from the naked mole rat, and that treatment with 3-MA or rapamycin increased the ratio of apoptotic cells to normal hepatic stellate cells.

  19. INK4 locus of the tumor-resistant rodent, the naked mole rat, expresses a functional p15/p16 hybrid isoform.

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    Tian, Xiao; Azpurua, Jorge; Ke, Zhonghe; Augereau, Adeline; Zhang, Zhengdong D; Vijg, Jan; Gladyshev, Vadim N; Gorbunova, Vera; Seluanov, Andrei

    2015-01-27

    The naked mole rat (Heterocephalus glaber) is a long-lived and tumor-resistant rodent. Tumor resistance in the naked mole rat is mediated by the extracellular matrix component hyaluronan of very high molecular weight (HMW-HA). HMW-HA triggers hypersensitivity of naked mole rat cells to contact inhibition, which is associated with induction of the INK4 (inhibitors of cyclin dependent kinase 4) locus leading to cell-cycle arrest. The INK4a/b locus is among the most frequently mutated in human cancer. This locus encodes three distinct tumor suppressors: p15(INK4b), p16(INK4a), and ARF (alternate reading frame). Although p15(INK4b) has its own ORF, p16(INK4a) and ARF share common second and third exons with alternative reading frames. Here, we show that, in the naked mole rat, the INK4a/b locus encodes an additional product that consists of p15(INK4b) exon 1 joined to p16(INK4a) exons 2 and 3. We have named this isoform pALT(INK4a/b) (for alternative splicing). We show that pALT(INK4a/b) is present in both cultured cells and naked mole rat tissues but is absent in human and mouse cells. Additionally, we demonstrate that pALT(INK4a/b) expression is induced during early contact inhibition and upon a variety of stresses such as UV, gamma irradiation-induced senescence, loss of substrate attachment, and expression of oncogenes. When overexpressed in naked mole rat or human cells, pALT(INK4a/b) has stronger ability to induce cell-cycle arrest than either p15(INK4b) or p16(INK4a). We hypothesize that the presence of the fourth product, pALT(INK4a/b) of the INK4a/b locus in the naked mole rat, contributes to the increased resistance to tumorigenesis of this species.

  20. The Insulin-Like Growth Factor System in the Long-Lived Naked Mole-Rat

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    Brohus, Malene; Gorbunova, Vera; Faulkes, Chris G.; Overgaard, Michael T.; Conover, Cheryl A.

    2015-01-01

    Naked mole-rats (Heterocephalus glaber) (NMRs) are the longest living rodents known. They show negligible senescence, and are resistant to cancers and certain damaging effects associated with aging. The insulin-like growth factors (IGFs) have pluripotent actions, influencing growth processes in virtually every system of the body. They are established contributors to the aging process, confirmed by the demonstration that decreased IGF signaling results in life-extending effects in a variety of species. The IGFs are likewise involved in progression of cancers by mediating survival signals in malignant cells. This report presents a full characterization of the IGF system in the NMR: ligands, receptors, IGF binding proteins (IGFBPs), and IGFBP proteases. A particular emphasis was placed on the IGFBP protease, pregnancy-associated plasma protein-A (PAPP-A), shown to be an important lifespan modulator in mice. Comparisons of IGF-related genes in the NMR with human and murine sequences indicated no major differences in essential parts of the IGF system, including PAPP-A. The protease was shown to possess an intact active site despite the report of a contradictory genome sequence. Furthermore, PAPP-A was expressed and translated in NMRs cells and retained IGF-dependent proteolytic activity towards IGFBP-4 and IGF-independent activity towards IGFBP-5. However, experimental data suggest differential regulatory mechanisms for PAPP-A expression in NMRs than those described in humans and mice. This overall description of the IGF system in the NMR represents an initial step towards elucidating the complex molecular mechanisms underlying longevity, and how these animals have evolved to ensure a delayed and healthy aging process. PMID:26694858

  1. The Insulin-Like Growth Factor System in the Long-Lived Naked Mole-Rat.

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    Malene Brohus

    Full Text Available Naked mole-rats (Heterocephalus glaber (NMRs are the longest living rodents known. They show negligible senescence, and are resistant to cancers and certain damaging effects associated with aging. The insulin-like growth factors (IGFs have pluripotent actions, influencing growth processes in virtually every system of the body. They are established contributors to the aging process, confirmed by the demonstration that decreased IGF signaling results in life-extending effects in a variety of species. The IGFs are likewise involved in progression of cancers by mediating survival signals in malignant cells. This report presents a full characterization of the IGF system in the NMR: ligands, receptors, IGF binding proteins (IGFBPs, and IGFBP proteases. A particular emphasis was placed on the IGFBP protease, pregnancy-associated plasma protein-A (PAPP-A, shown to be an important lifespan modulator in mice. Comparisons of IGF-related genes in the NMR with human and murine sequences indicated no major differences in essential parts of the IGF system, including PAPP-A. The protease was shown to possess an intact active site despite the report of a contradictory genome sequence. Furthermore, PAPP-A was expressed and translated in NMRs cells and retained IGF-dependent proteolytic activity towards IGFBP-4 and IGF-independent activity towards IGFBP-5. However, experimental data suggest differential regulatory mechanisms for PAPP-A expression in NMRs than those described in humans and mice. This overall description of the IGF system in the NMR represents an initial step towards elucidating the complex molecular mechanisms underlying longevity, and how these animals have evolved to ensure a delayed and healthy aging process.

  2. Specific Paucity of Unmyelinated C-Fibers in Cutaneous Peripheral Nerves of the African Naked-Mole Rat: Comparative Analysis Using Six Species of Bathyergidae

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    Smith, Ewan S; Purfürst, Bettina; Grigoryan, Tamara; Park, Thomas J; Bennett, Nigel C; Lewin, Gary R

    2012-01-01

    In mammalian peripheral nerves, unmyelinated C-fibers usually outnumber myelinated A-fibers. By using transmission electron microscopy, we recently showed that the saphenous nerve of the naked mole-rat (Heterocephalus glaber) has a C-fiber deficit manifested as a substantially lower C:A-fiber ratio compared with other mammals. Here we determined the uniqueness of this C-fiber deficit by performing a quantitative anatomical analysis of several peripheral nerves in five further members of the Bathyergidae mole-rat family: silvery (Heliophobius argenteocinereus), giant (Fukomys mechowii), Damaraland (Fukomys damarensis), Mashona (Fukomys darlingi), and Natal (Cryptomys hottentotus natalensis) mole-rats. In the largely cutaneous saphenous and sural nerves, the naked mole-rat had the lowest C:A-fiber ratio (∼1.5:1 compared with ∼3:1), whereas, in nerves innervating both skin and muscle (common peroneal and tibial) or just muscle (lateral/medial gastrocnemius), this pattern was mostly absent. We asked whether lack of hair follicles alone accounts for the C-fiber paucity by using as a model a mouse that loses virtually all its hair as a consequence of conditional deletion of the β-catenin gene in the skin. These β-catenin loss-of function mice (β-cat LOF mice) displayed only a mild decrease in C:A-fiber ratio compared with wild-type mice (4.42 compared with 3.81). We suggest that the selective cutaneous C-fiber deficit in the cutaneous nerves of naked mole-rats is unlikely to be due primarily to lack of skin hair follicles. Possible mechanisms contributing to this unique peripheral nerve anatomy are discussed. J. Comp. Neurol. 520:2785–2803, 2012. © 2012 Wiley Periodicals, Inc. PMID:22528859

  3. Comparative analysis of genome maintenance genes in naked mole rat, mouse, and human

    NARCIS (Netherlands)

    S.L. Macrae (Sheila L.); Q. Zhang (Quanwei); C. Lemetre (Christophe); I. Seim (Inge); R.B. Calder (Robert B.); J.H.J. Hoeijmakers (Jan); Y. Suh (Yousin); V.N. Gladyshev (Vadim N.); A. Seluanov (Andrei); V. Gorbunova (Vera); J. Vijg (Jan); Z.D. Zhang (Zhengdong D.)

    2015-01-01

    textabstractGenome maintenance (GM) is an essential defense system against aging and cancer, as both are characterized by increased genome instability. Here, we compared the copy number variation and mutation rate of 518 GM-associated genes in the naked mole rat (NMR), mouse, and human genomes. GM g

  4. Blunted behavioral and c Fos responses to acidic fumes in the African naked mole-rat.

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    Pamela Colleen LaVinka

    Full Text Available Acidosis in the skin triggers activation of pain pathways and behaviors indicative of pain in vertebrates. The exception is the naked mole-rat, the only known vertebrate to show physiological and behavioral insensitivity to acid pain in the skin. The goal of the present study was to determine behavioral and physiological responses of this species to airborne acidic fumes, which would be expected to affect the trigeminal pain pathway in other species. Behaviorally, naked mole-rats did not avoid fumes from moderately high concentrations of acetic acid (10 and 20%, and c Fos labeling showed no increase in activity in the trigeminal nuclei and nucleus tractus solitarius. In contrast, these concentrations triggered behavioral aversion and increased Fos activity in other laboratory rodents. For a very high concentration of acetic acid (50%, naked mole-rats showed significant avoidance behavior and increased Fos labeling in the nucleus tractus solitarius caudal region, which receives vagal chemosensory information. However, there was no increase in trigeminal labeling, and in fact, activity significantly decreased. This pattern is opposite of that associated with another irritant, ammonia fumes, which elicited an increase in trigeminal but not nucleus tractus solitarius Fos labeling, and no behavioral avoidance. Behavioral avoidance of acidic fumes, but no increased labeling in the trigeminal pain nucleus is consistent with the notion of adaptations to blunt acid pain, which would be advantageous for naked mole-rats as they normally live under chronically high levels of acidosis-inducing CO(2.

  5. A distinct role of the queen in coordinated workload and soil distribution in eusocial naked mole-rats.

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    Nobuyuki Kutsukake

    Full Text Available We investigated how group members achieve collective decision-making, by considering individual intrinsic behavioural rules and behavioural mechanisms for maintaining social integration. Using a simulated burrow environment, we investigated the behavioural rules of coordinated workload for soil distribution in a eusocial mammal, the naked mole-rat (Heterocephalus glaber. We tested two predictions regarding a distinct role of the queen, a socially dominant individual in the caste system: the presence of a queen would increase the workload of other caste individuals, and the cues by a queen would affect the soil distribution. In experiment 1, we placed four individuals of various castes from the same colony into an experimental burrow. Workers exhibited the highest frequency of workload compared to other castes. The presence of a queen activated the workload by other individuals. Individuals showed a consistent workload in a particular direction so as to bias the soil distribution. These results suggest that individuals have a consensus on soil distribution and that the queen plays a distinct role. In experiment 2, we placed the odour of a queen in one of four cells and observed its effect on other individuals' workload and soil distribution. Relative to other cells, individuals frequently dug in the queen cell so the amount of soil in the queen cell decreased. These results suggest that queen odour is an important cue in coordinated workload and soil distribution in this species.

  6. Hypofunctional TrkA Accounts for the Absence of Pain Sensitization in the African Naked Mole-Rat

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    Damir Omerbašić

    2016-10-01

    Full Text Available The naked mole-rat is a subterranean rodent lacking several pain behaviors found in humans, rats, and mice. For example, nerve growth factor (NGF, an important mediator of pain sensitization, fails to produce thermal hyperalgesia in naked mole-rats. The sensitization of capsaicin-sensitive TRPV1 ion channels is necessary for NGF-induced hyperalgesia, but naked mole-rats have fully functional TRPV1 channels. We show that exposing isolated naked mole-rat nociceptors to NGF does not sensitize TRPV1. However, the naked mole-rat NGF receptor TrkA displays a reduced ability to engage signal transduction pathways that sensitize TRPV1. Between one- and three-amino-acid substitutions in the kinase domain of the naked mole-rat TrkA are sufficient to render the receptor hypofunctional, and this is associated with the absence of heat hyperalgesia. Our data suggest that evolution has selected for a TrkA variant that abolishes a robust nociceptive behavior in this species but is still compatible with species fitness.

  7. Multidimensional MRI-CT atlas of the naked mole-rat brain

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    Fumiko eSeki

    2013-12-01

    Full Text Available Naked mole-rats have a variety of distinctive features such as the organisation of a hierarchical society (known as eusociality, extraordinary longevity, and cancer resistance; thus, it would be worthwhile investigating these animals in detail. One important task is the preparation of a brain atlas database that provide comprehensive information containing multidimensional data with various image contrasts, which can be achievable using a magnetic resonance imaging (MRI. Advanced MRI techniques such as diffusion tensor imaging (DTI, which generates high contrast images of fibre structures, can characterise unique morphological properties in addition to conventional MRI. To obtain high spatial resolution images, MR histology, DTI, and X-ray computed tomography (CT were performed on the fixed adult brain. Skull and brain structures were segmented as well as reconstructed in stereotaxic coordinates. Data were also acquired for the neonatal brain to allow developmental changes to be observed. Moreover, in vivo imaging of naked mole-rats was established as an evaluation tool of live animals. The data obtained comprised three-dimensional (3D images with high tissue contrast as well as stereotaxic coordinates. Developmental differences in the visual system were highlighted in particular by DTI. Although it was difficult to delineate optic nerves in the mature adult brain, parts of them could be distinguished in the immature neonatal brain. From observation of cortical thickness, possibility of high somatosensory system development replaced to the visual system was indicated. 3D visualisation of brain structures in the atlas as well as the establishment of in vivo imaging would promote neuroimaging researches towards detection of novel characteristics of eusocial naked mole-rats.

  8. Immunohistochemical characterization of the chemosensory pulmonary neuroepithelial bodies in the naked mole-rat reveals a unique adaptive phenotype.

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    Jie Pan

    Full Text Available The pulmonary neuroepithelial bodies (NEBs constitute polymodal airway chemosensors for monitoring and signaling ambient gas concentrations (pO2, pCO2/H+ via complex innervation to the brain stem controlling breathing. NEBs produce the bioactive amine, serotonin (5-HT, and a variety of peptides with multiple effects on lung physiology and other organ systems. NEBs in mammals appear prominent and numerous during fetal and neonatal periods, and decline in the post-natal period suggesting an important role during perinatal adaptation. The naked mole-rat (NMR, Heterocephalus glaber, has adapted to the extreme environmental conditions of living in subterranean burrows in large colonies (up to 300 colony mates. The crowded, unventilated burrows are environments of severe hypoxia and hypercapnia. However, NMRs adjust readily to above ground conditions. The chemosensory NEBs of this species were characterized and compared to those of the conventional Wistar rat (WR to identify similarities and differences that could explain the NMR's adaptability to environments. A multilabel immunohistochemical analysis combined with confocal microscopy revealed that the expression patterns of amine, peptide, neuroendocrine, innervation markers and chemosensor component proteins in NEBs of NMR were similar to that of WR. However, we found the following differences: 1 NEBs in both neonatal and adult NMR lungs were significantly larger and more numerous as compared to WR; 2 NEBs in NMR had a more variable compact cell organization and exhibited significant differences in the expression of adhesion proteins; 3 NMR NEBs showed a significantly greater ratio of 5-HT positive cells with an abundance of 5-HT; 4 NEBs in NMR expressed the proliferating cell nuclear antigen (PCNA and the neurogenic gene (MASH1 indicating active proliferation and a state of persistent differentiation. Taken together our findings suggest that NEBs in lungs of NMR are in a hyperactive, functional

  9. Comparative analysis of genome maintenance genes in naked mole rat, mouse, and human

    Science.gov (United States)

    MacRae, Sheila L; Zhang, Quanwei; Lemetre, Christophe; Seim, Inge; Calder, Robert B; Hoeijmakers, Jan; Suh, Yousin; Gladyshev, Vadim N; Seluanov, Andrei; Gorbunova, Vera; Vijg, Jan; Zhang, Zhengdong D

    2015-01-01

    Genome maintenance (GM) is an essential defense system against aging and cancer, as both are characterized by increased genome instability. Here, we compared the copy number variation and mutation rate of 518 GM-associated genes in the naked mole rat (NMR), mouse, and human genomes. GM genes appeared to be strongly conserved, with copy number variation in only four genes. Interestingly, we found NMR to have a higher copy number of CEBPG, a regulator of DNA repair, and TINF2, a protector of telomere integrity. NMR, as well as human, was also found to have a lower rate of germline nucleotide substitution than the mouse. Together, the data suggest that the long-lived NMR, as well as human, has more robust GM than mouse and identifies new targets for the analysis of the exceptional longevity of the NMR. PMID:25645816

  10. Functional neurokinin and NMDA receptor activity in an animal naturally lacking substance P: the naked mole-rat.

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    Antje Brand

    Full Text Available Naked mole-rats are extremely unusual among mammals in that their cutaneous C-fibers lack the neuropeptide Substance P (SP. In other mammals, SP plays an important role in nociception: it is released from C-fibers onto spinal neurons where it facilitates NMDA receptor activity and causes sensitization that can last for minutes, hours or days. In the present study, we tested the effects of intrathecal application of: 1 SP, 2 an SP antagonist (GR-82334, and 3 an NMDA antagonist (APV on heat-evoked foot withdrawal. In the naked mole-rat, at a high enough concentration, application of SP caused a large, immediate, and long-lasting sensitization of foot withdrawal latency that was transiently reversed by application of either antagonist. However, neither SP nor NMDA antagonists had an effect when administered alone to naïve animals. In contrast, both antagonists induced an increase in basal withdrawal latency in mice. These results indicate that spinal neurons in naked mole-rats have functional SP and NMDA receptors, but that these receptors do not participate in heat-evoked foot withdrawal unless SP is experimentally introduced. We propose that the natural lack of SP in naked mole-rat C-fibers may have resulted during adaptation to living in a chronically high carbon dioxide, high ammonia environment that, in other mammals, would stimulate C-fibers and evoke nocifensive behavior.

  11. Functional characteristics of the naked mole rat μ-opioid receptor.

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    Melanie Busch-Dienstfertig

    Full Text Available While humans and most animals respond to µ-opioid receptor (MOR agonists with analgesia and decreased aggression, in the naked mole rat (NMR opioids induce hyperalgesia and severe aggression. Single nucleotide polymorphisms in the human mu-opioid receptor gene (OPRM1 can underlie altered behavioral responses to opioids. Therefore, we hypothesized that the primary structure of the NMR MOR may differ from other species. Sequencing of the NMR oprm1 revealed strong homology to other mammals, but exposed three unique amino acids that might affect receptor-ligand interactions. The NMR and rat oprm1 sequences were cloned into mammalian expression vectors and transfected into HEK293 cells. Radioligand binding and 3'-5'-cyclic adenosine monophosphate (cAMP enzyme immunoassays were used to compare opioid binding and opioid-mediated cAMP inhibition. At normalized opioid receptor protein levels we detected significantly lower [3H]DAMGO binding to NMR compared to rat MOR, but no significant difference in DAMGO-induced cAMP inhibition. Strong DAMGO-induced MOR internalization was detectable using radioligand binding and confocal imaging in HEK293 cells expressing rat or NMR receptor, while morphine showed weak or no effects. In summary, we found minor functional differences between rat and NMR MOR suggesting that other differences e.g. in anatomical distribution of MOR underlie the NMR's extreme reaction to opioids.

  12. Low sulfide levels and a high degree of cystathionine β-synthase (CBS) activation by S-adenosylmethionine (SAM) in the long-lived naked mole-rat.

    Science.gov (United States)

    Dziegelewska, Maja; Holtze, Susanne; Vole, Christiane; Wachter, Ulrich; Menzel, Uwe; Morhart, Michaela; Groth, Marco; Szafranski, Karol; Sahm, Arne; Sponholz, Christoph; Dammann, Philip; Huse, Klaus; Hildebrandt, Thomas; Platzer, Matthias

    2016-08-01

    Hydrogen sulfide (H2S) is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS), an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  13. Low sulfide levels and a high degree of cystathionine β-synthase (CBS activation by S-adenosylmethionine (SAM in the long-lived naked mole-rat

    Directory of Open Access Journals (Sweden)

    Maja Dziegelewska

    2016-08-01

    Full Text Available Hydrogen sulfide (H2S is a gaseous signalling molecule involved in many physiological and pathological processes. There is increasing evidence that H2S is implicated in aging and lifespan control in the diet-induced longevity models. However, blood sulfide concentration of naturally long-lived species is not known. Here we measured blood sulfide in the long-lived naked mole-rat and five other mammalian species considerably differing in lifespan and found a negative correlation between blood sulfide and maximum longevity residual. In addition, we show that the naked mole-rat cystathionine β-synthase (CBS, an enzyme whose activity in the liver significantly contributes to systemic sulfide levels, has lower activity in the liver and is activated to a higher degree by S-adenosylmethionine compared to other species. These results add complexity to the understanding of the role of H2S in aging and call for detailed research on naked mole-rat transsulfuration.

  14. Application prospects of naked mole rats in biomedical research%裸鼹鼠在生物医学研究中的应用前景

    Institute of Scientific and Technical Information of China (English)

    崔淑芳

    2016-01-01

    裸鼹鼠是一种奇特的动物,具有寿命长、抗肿瘤、耐缺氧、新陈代谢率低、痛觉缺失、触觉灵敏、视觉功能低下、骨骼再生能力强等诸多特点。本文在概述裸鼹鼠上述生物学特点的基础上,结合当前肿瘤、衰老、低氧适应以及疼痛等领域研究趋势,对裸鼹鼠在生物医学研究中的应用前景进行分析与展望。%Naked mole rats are unique animals with long lifetime, anti-tumor properties, hypoxia tolerance, low metabolic rate, analgesia, tactile sensitiveness, poor eyesight and strong bone regeneration ability. Based on the basic bio-logical characteristics of naked mole rats, and the current research trends in the field of cancer, aging, hypoxia tolerance, as well as algesia, this review focuses on the application prospects of naked mole rats in biomedical research.

  15. Lack of sexual dimorphism in femora of the eusocial and hypogonadic naked mole-rat: a novel animal model for the study of delayed puberty on the skeletal system.

    Science.gov (United States)

    Pinto, M; Jepsen, K J; Terranova, C J; Buffenstein, R

    2010-01-01

    Sex steroid hormones are major determinants of bone morphology and quality and are responsible for sexually dimorphic skeletal traits. Hypogonadism results in suboptimal skeletal development and may lead to an increased risk of bone fracture later in life. The etiology of delayed puberty and/or hypothalamic amenorrhea is poorly understood, and experimental animal models addressing this issue are predominantly based upon short-term experimental induction of hormonal suppression via gonadotropin releasing hormone antagonists (GnRH-a). This acute change in hormone profile does not necessarily emulate the natural progression of hypogonadic bone disorders. We propose a novel animal model with which to explore the effects of chronic hypogonadism on bone quality, the naked mole-rat (NMR; Heterocephalus glaber). This mouse-size rodent may remain reproductively suppressed throughout its life, if it remains as a subordinate within the eusocial mole-rat colony. NMRs live in large colonies with a single dominant breeding female. She, primarily by using aggressive social contact, naturally suppresses the hypothalamic gonadotropic axis of subordinate NMRs and thereby their reproductive expression. However, should an NMR be separated from the dominant breeder, within less than a week reproductive hormones may become elevated and the animal attains breeding status. We questioned if sexual suppression of subordinates impact upon the development and maintenance of the femora and lead to a sexually indistinct monomorphic skeleton. Femora were obtained from male and female NMRs that were either non-breeders (subordinate) or breeders at the time of sacrifice. Diaphyseal cross-sectional morphology, metaphyseal trabecular micro-architecture and tissue mineral density of the femur were measured using microcomputed tomography and diaphyseal mechanical properties were assessed by four-point bending tests to failure. Subordinates were sexually monomorphic and showed no significant

  16. Cancer resistance in the blind mole rat is mediated by concerted necrotic cell death mechanism.

    Science.gov (United States)

    Gorbunova, Vera; Hine, Christopher; Tian, Xiao; Ablaeva, Julia; Gudkov, Andrei V; Nevo, Eviatar; Seluanov, Andrei

    2012-11-20

    Blind mole rats Spalax (BMR) are small subterranean rodents common in the Middle East. BMR is distinguished by its adaptations to life underground, remarkable longevity (with a maximum documented lifespan of 21 y), and resistance to cancer. Spontaneous tumors have never been observed in spalacids. To understand the mechanisms responsible for this resistance, we examined the growth of BMR fibroblasts in vitro of the species Spalax judaei and Spalax golani. BMR cells proliferated actively for 7-20 population doublings, after which the cells began secreting IFN-β, and the cultures underwent massive necrotic cell death within 3 d. The necrotic cell death phenomenon was independent of culture conditions or telomere shortening. Interestingly, this cell behavior was distinct from that observed in another long-lived and cancer-resistant African mole rat, Heterocephalus glaber, the naked mole rat in which cells display hypersensitivity to contact inhibition. Sequestration of p53 and Rb proteins using SV40 large T antigen completely rescued necrotic cell death. Our results suggest that cancer resistance of BMR is conferred by massive necrotic response to overproliferation mediated by p53 and Rb pathways, and triggered by the release of IFN-β. Thus, we have identified a unique mechanism that contributes to cancer resistance of this subterranean mammal extremely adapted to life underground.

  17. Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.

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    René Thieme

    Full Text Available The naked mole-rat (NMR is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M.Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL in NMR compared to human. NMR-A2M can be transformed by methylamine and trypsin resulting in a conformational change similar to human A2M. NMR-A2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma.We found transformed NMR-A2M binding to its specific receptor LRP1. We could demonstrate lower protein expression of LRP1 in the NMR liver tissue compared to human but higher expression of A2M. This was accompanied by a higher EpCAM protein expression as central adhesion molecule in cancer progression. NMR-plasma was capable to increase the adhesion in human fibroblast in vitro most probably by increasing CD29 protein expression. This is the first report, demonstrating similarities as well as distinct differences between A2M in NMR and human plasma. This might be directly linked to the intriguing phenotype of the NMR and suggests that A2M might probably play an important role in anti-cancer and the

  18. Analysis of Alpha-2 Macroglobulin from the Long-Lived and Cancer-Resistant Naked Mole-Rat and Human Plasma.

    Science.gov (United States)

    Thieme, René; Kurz, Susanne; Kolb, Marlen; Debebe, Tewodros; Holtze, Susanne; Morhart, Michaela; Huse, Klaus; Szafranski, Karol; Platzer, Matthias; Hildebrandt, Thomas B; Birkenmeier, Gerd

    2015-01-01

    The naked mole-rat (NMR) is a long-lived and cancer resistant species. Identification of potential anti-cancer and age related mechanisms is of great interest and makes this species eminent to investigate anti-cancer strategies and understand aging mechanisms. Since it is known that the NMR expresses higher liver mRNA-levels of alpha 2-macroglobulin than mice, nothing is known about its structure, functionality or expression level in the NMR compared to the human A2M. Here we show a comprehensive analysis of NMR- and human plasma-A2M, showing a different prediction in glycosylation of NMR-A2M, which results in a higher molecular weight compared to human A2M. Additionally, we found a higher concentration of A2M (8.3±0.44 mg/mL vs. and 4.4±0.20 mg/mL) and a lower total plasma protein content (38.7±1.79 mg/mL vs. 61.7±3.20 mg/mL) in NMR compared to human. NMR-A2M can be transformed by methylamine and trypsin resulting in a conformational change similar to human A2M. NMR-A2M is detectable by a polyclonal antibody against human A2M. Determination of tryptic and anti-tryptic activity of NMR and human plasma revealed a higher anti-tryptic activity of the NMR plasma. On the other hand, less proteolytic activity was found in NMR plasma compared to human plasma. We found transformed NMR-A2M binding to its specific receptor LRP1. We could demonstrate lower protein expression of LRP1 in the NMR liver tissue compared to human but higher expression of A2M. This was accompanied by a higher EpCAM protein expression as central adhesion molecule in cancer progression. NMR-plasma was capable to increase the adhesion in human fibroblast in vitro most probably by increasing CD29 protein expression. This is the first report, demonstrating similarities as well as distinct differences between A2M in NMR and human plasma. This might be directly linked to the intriguing phenotype of the NMR and suggests that A2M might probably play an important role in anti-cancer and the anti

  19. Adaptations to a Subterranean Environment and Longevity Revealed by the Analysis of Mole Rat Genomes

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    Xiaodong Fang

    2014-09-01

    Full Text Available Subterranean mammals spend their lives in dark, unventilated environments that are rich in carbon dioxide and ammonia and low in oxygen. Many of these animals are also long-lived and exhibit reduced aging-associated diseases, such as neurodegenerative disorders and cancer. We sequenced the genome of the Damaraland mole rat (DMR, Fukomys damarensis and improved the genome assembly of the naked mole rat (NMR, Heterocephalus glaber. Comparative genome analyses, along with the transcriptomes of related subterranean rodents, revealed candidate molecular adaptations for subterranean life and longevity, including a divergent insulin peptide, expression of oxygen-carrying globins in the brain, prevention of high CO2-induced pain perception, and enhanced ammonia detoxification. Juxtaposition of the genomes of DMR and other more conventional animals with the genome of NMR revealed several truly exceptional NMR features: unusual thermogenesis, an aberrant melatonin system, pain insensitivity, and unique processing of 28S rRNA. Together, these genomes and transcriptomes extend our understanding of subterranean adaptations, stress resistance, and longevity.

  20. Trading new neurons for status: Adult hippocampal neurogenesis in eusocial Damaraland mole-rats.

    Science.gov (United States)

    Oosthuizen, M K; Amrein, I

    2016-06-02

    Diversity in social structures, from solitary to eusocial, is a prominent feature of subterranean African mole-rat species. Damaraland mole-rats are eusocial, they live in colonies that are characterized by a reproductive division of labor and a subdivision into castes based on physiology and behavior. Damaraland mole-rats are exceptionally long lived and reproductive animals show delayed aging compared to non-reproductive animals. In the present study, we described the hippocampal architecture and the rate of hippocampal neurogenesis of wild-derived, adult Damaraland mole-rats in relation to sex, relative age and social status or caste. Overall, Damaraland mole-rats were found to have a small hippocampus and low rates of neurogenesis. We found no correlation between neurogenesis and sex or relative age. Social status or caste was the most prominent modulator of neurogenesis. An inverse relationship between neurogenesis and social status was apparent, with queens displaying the lowest neurogenesis while the worker mole-rats had the most. As there is no natural progression from one caste to another, social status within a colony was relatively stable and is reflected in the level of neurogenesis. Our results correspond to those found in the naked mole-rat, and may reflect an evolutionary and environmentally conserved trait within social mole-rat species.

  1. Structural and functional differences of the reproductive system in fertile and infertile male naked mole rats%雄性裸鼹鼠可育个体与不可育个体生殖系统结构与功能差异初探

    Institute of Scientific and Technical Information of China (English)

    余琛琳; 林丽芳; 袁子彦; 赵善民; 汤球; 孙伟; 蔡丽萍; 徐晨; 崔淑芳

    2016-01-01

    目的:观察和分析雄性裸鼹鼠中可育个体和不可育个体生殖系统结构和功能的差异,初步探索雄性不可育个体不能繁育后代的原因。方法雄性裸鼹鼠可育个体和不可育个体各5只,先采集一侧睾丸和附睾组织,称重后换算脏器系数;再采集另一侧睾丸组织,用中性甲醛溶液进行固定后制备组织切片;取另一侧附睾用作精子计数、精子活动度的测定;采集血清测定血清中黄体生成素( LH)和睾酮( T)浓度。结果与可育个体组相比较,不可育个体组的睾丸重量下降(P<0.05),附睾重量显著下降(P<0.01),且睾丸系数和附睾系数均显著下降(P<0.01);精子数量显著减少(P<0.01),精子活动度也显著减弱(P<0.01);血清黄体生成素和睾酮水平均显著降低(P<0.01);睾丸组织切片观察显示,不可育个体各级生精细胞严重脱落,排列混乱无序,支持细胞和间质细胞均有减少。结论雄性裸鼹鼠不可育个体生殖系统存在睾丸和附睾萎缩、生精功能下降和性激素分泌减少等现象。%Objective The aim of this study was to observe and analyze the differences of reproductive system in fertile and infertile male naked mole rats. Methods Five fertile and 5 infertile 2-5-year old male naked mole rats were used in this study. The testis and epididymis on one side were collected, weighted, and measured to get their viscera coeffi-cient. The other side testicle was collected and fixed in neutral formalin solution for histological examination. The other side epididymis was used to examine the sperm count and sperm activity. Serum of the naked mole rats was collected to measure the level of luteinizing hormone ( LH) and testosterone ( T) . Results Compared with the fertile group, the testis weight of infertile group was significantly decreased (P<0. 05), their epididymis weight, testicular coefficient and the

  2. Life in Burrows Channelled the Morphological Evolution of the Skull in Rodents: the Case of African Mole-Rats (Bathyergidae, Rodentia)

    OpenAIRE

    2015-01-01

    International audience; African mole-rats are fossorial rodents that consist of five chisel-tooth digging genera (Heterocephalus, Heliophobius, Georychus, Fukomys, and Cryptomys) and one scratch digger (Bathyergus). They are characterized by striking physiological, morphological, and behavioral adaptations intimately related to their subterranean life. The influence of their mode of life in shaping the cranial morphology has yet to be evaluated in comparison to other Ctenohystrica, especially...

  3. Naked Mole Rat Longevity — Possible Mechanism Identified —

    Science.gov (United States)

    University of Rochester researchers, who previously discovered a mechanism that may contribute to the species’ resistance to tumor formation, have announced that ribosome function may help account for the rats’ longevity.

  4. The colonic groove or furrow: a comparative morphological study of six species of African mole-rats (Rodentia, Bathyergidae).

    Science.gov (United States)

    Kotzé, S H; van der Merwe, E L; Ndou, R; O'Riain, M J; Bennett, N C

    2009-08-01

    Herbivorous mammals such as nutrias, guinea pigs, chinchillas, and mole-rats have a longitudinal mucosal colonic groove (furrow) in their ascending colon, which is thought to play a role in the colonic separation mechanism (CSM). It is not known whether this groove is structurally modified to adapt to this function in mole-rat species. The morphology of this groove was studied in 32 mol-rats, four species, one of which consisted of three subspecies, endemic to southern Africa and two species found in eastern Africa. The macroscopic morphology of the groove was documented, and samples for histological examination were taken. The groove was wide at its origin at the cecocolic junction and was lined on either side by a row of papillae with the opposing papillae slightly offset in arrangement. The papillated groove gradually decreased in size toward the distal part of the ascending colon where it disappeared. This pattern was similar in all species except in Heterocephalus glaber, where the papillae were absent and the groove was lined by two longitudinal ridges. A histological examination of cross sections revealed that the mucosa covering the inner and outer walls of the groove was rich in mucous-secreting goblet cells. The walls of the groove contained smooth muscle extending from the inner circular smooth muscle layer at the base to the tips of the papillae in all species examined as well as arteries, lymphatic vessels, and prominent sinusoid-like veins. The groove could be demonstrated both macroscopically and histologically in three Bathyergus suillus fetuses of varying sizes. The sinusoid-like veins present in all grooves, regardless of macroscopic shape, suggest that they have a role in the functioning of the groove. (c) 2009 Wiley-Liss, Inc.

  5. Possible incipient sympatric ecological speciation in blind mole rats (Spalax)

    Science.gov (United States)

    Hadid, Yarin; Tzur, Shay; Pavlíček, Tomáš; Šumbera, Radim; Šklíba, Jan; Lövy, Matěj; Fragman-Sapir, Ori; Beiles, Avigdor; Arieli, Ran; Raz, Shmuel; Nevo, Eviatar

    2013-01-01

    Sympatric speciation has been controversial since it was first proposed as a mode of speciation. Subterranean blind mole rats (Spalacidae) are considered to speciate allopatrically or peripatrically. Here, we report a possible incipient sympatric adaptive ecological speciation in Spalax galili (2n = 52). The study microsite (0.04 km2) is sharply subdivided geologically, edaphically, and ecologically into abutting barrier-free ecologies divergent in rock, soil, and vegetation types. The Pleistocene Alma basalt abuts the Cretaceous Senonian Kerem Ben Zimra chalk. Only 28% of 112 plant species were shared between the soils. We examined mitochondrial DNA in the control region and ATP6 in 28 mole rats from basalt and in 14 from chalk habitats. We also sequenced the complete mtDNA (16,423 bp) of four animals, two from each soil type. Remarkably, the frequency of all major haplotype clusters (HC) was highly soil-biased. HCI and HCII are chalk biased. HC-III was abundant in basalt (36%) but absent in chalk; HC-IV was prevalent in basalt (46.5%) but was low (20%) in chalk. Up to 40% of the mtDNA diversity was edaphically dependent, suggesting constrained gene flow. We identified a homologous recombinant mtDNA in the basalt/chalk studied area. Phenotypically significant divergences differentiate the two populations, inhabiting different soils, in adaptive oxygen consumption and in the amount of outside-nest activity. This identification of a possible incipient sympatric adaptive ecological speciation caused by natural selection indirectly refutes the allopatric alternative. Sympatric ecological speciation may be more prevalent in nature because of abundant and sharply abutting divergent ecologies. PMID:23359700

  6. The King of Denmark and the Naked Mole Rat: Teaching Critical Thinking for Social Justice

    Science.gov (United States)

    McLaughlin, Danielle

    2012-01-01

    Asking hard questions is just that--hard. But if we are truly committed to teaching for social justice, we need to encourage our children to find as many points of view as they can, and to ask questions we may never be able to answer, knowing that education for citizenship lies in the process of thinking critically about the many sides of a…

  7. Eye development in the Cape dune mole rat.

    Science.gov (United States)

    Nikitina, Natalya V; Kidson, Susan H

    2014-03-01

    Studies on mammalian species with naturally reduced eyes can provide valuable insights into the evolutionary developmental mechanisms underlying the reduction of the eye structures. Because few naturally microphthalmic animals have been studied and eye reduction must have evolved independently in many of the modern groups, novel evolutionary developmental models for eye research have to be sought. Here, we present a first report on embryonic eye development in the Cape dune mole rat, Bathyergus suillus. The eyes of these animals contain all the internal structures characteristic of the normal eye but exhibit abnormalities in the anterior chamber structures. The lens is small but develops normally and exhibits a normal expression of α- and γ-crystallins. One of the interesting features of these animals is an extremely enlarged and highly pigmented ciliary body. In order to understand the molecular basis of this unusual feature, the expression pattern of an early marker of the ciliary zone, Ptmb4, was investigated in this animal. Surprisingly, in situ hybridization results revealed that Ptmb4 expression was absent from the ciliary body zone of the developing Bathyergus eye.

  8. Is evolution of blind mole rats determined by climate oscillations?

    Directory of Open Access Journals (Sweden)

    Yarin Hadid

    Full Text Available The concept of climate variability facilitating adaptive radiation supported by the "Court Jester" hypothesis is disputed by the "Red Queen" one, but the prevalence of one or the other might be scale-dependent. We report on a detailed, comprehensive phylo-geographic study on the ∼4 kb mtDNA sequence in underground blind mole rats of the family Spalacidae (or subfamily Spalacinae from the East Mediterranean steppes. Our study aimed at testing the presence of periodicities in branching patterns on a constructed phylogenetic tree and at searching for congruence between branching events, tectonic history and paleoclimates. In contrast to the strong support for the majority of the branching events on the tree, the absence of support in a few instances indicates that network-like evolution could exist in spalacids. In our tree, robust support was given, in concordance with paleontological data, for the separation of spalacids from muroid rodents during the first half of the Miocene when open, grass-dominated habitats were established. Marine barriers formed between Anatolia and the Balkans could have facilitated the separation of the lineage "Spalax" from the lineage "Nannospalax" and of the clade "leucodon" from the clade "xanthodon". The separation of the clade "ehrenbergi" occurred during the late stages of the tectonically induced uplift of the Anatolian high plateaus and mountains, whereas the separation of the clade "vasvarii" took place when the rapidly uplifting Taurus mountain range prevented the Mediterranean rainfalls from reaching the Central Anatolian Plateau. The separation of Spalax antiquus and S. graecus occurred when the southeastern Carpathians were uplifted. Despite the role played by tectonic events, branching events that show periodicity corresponding to 400-kyr and 100-kyr eccentricity bands illuminate the important role of orbital fluctuations on adaptive radiation in spalacids. At the given scale, our results supports the

  9. Extended Longevity of Reproductives Appears to be Common in Fukomys Mole-Rats (Rodentia, Bathyergidae)

    Science.gov (United States)

    Dammann, Philip; Šumbera, Radim; Maßmann, Christina; Scherag, André; Burda, Hynek

    2011-01-01

    African mole-rats (Bathyergidae, Rodentia) contain several social, cooperatively breeding species with low extrinsic mortality and unusually high longevity. All social bathyergids live in multigenerational families where reproduction is skewed towards a few breeding individuals. Most of their offspring remain as reproductively inactive “helpers” in their natal families, often for several years. This “reproductive subdivision” of mole-rat societies might be of interest for ageing research, as in at least one social bathyergid (Ansell's mole-rats Fukomys anselli), breeders have been shown to age significantly slower than non-breeders. These animals thus provide excellent conditions for studying the epigenetics of senescence by comparing divergent longevities within the same genotypes without the inescapable short-comings of inter-species comparisons. It has been claimed that many if not all social mole-rat species may have evolved similar ageing patterns, too. However, this remains unclear on account of the scarcity of reliable datasets on the subject. We therefore analyzed a 20-year breeding record of Giant mole-rats Fukomys mechowii, another social bathyergid species. We found that breeders indeed lived significantly longer than helpers (ca. 1.5–2.2fold depending on the sex), irrespective of social rank or other potentially confounding factors. Considering the phylogenetic positions of F. mechowii and F. anselli and unpublished data on a third Fukomys-species (F. damarensis) showing essentially the same pattern, it seems probable that the reversal of the classic trade-off between somatic maintenance and sexual reproduction is characteristic of the whole genus and hence of the vast majority of social mole-rats. PMID:21533255

  10. Extended longevity of reproductives appears to be common in Fukomys mole-rats (Rodentia, Bathyergidae.

    Directory of Open Access Journals (Sweden)

    Philip Dammann

    Full Text Available African mole-rats (Bathyergidae, Rodentia contain several social, cooperatively breeding species with low extrinsic mortality and unusually high longevity. All social bathyergids live in multigenerational families where reproduction is skewed towards a few breeding individuals. Most of their offspring remain as reproductively inactive "helpers" in their natal families, often for several years. This "reproductive subdivision" of mole-rat societies might be of interest for ageing research, as in at least one social bathyergid (Ansell's mole-rats Fukomys anselli, breeders have been shown to age significantly slower than non-breeders. These animals thus provide excellent conditions for studying the epigenetics of senescence by comparing divergent longevities within the same genotypes without the inescapable short-comings of inter-species comparisons. It has been claimed that many if not all social mole-rat species may have evolved similar ageing patterns, too. However, this remains unclear on account of the scarcity of reliable datasets on the subject. We therefore analyzed a 20-year breeding record of Giant mole-rats Fukomys mechowii, another social bathyergid species. We found that breeders indeed lived significantly longer than helpers (ca. 1.5-2.2fold depending on the sex, irrespective of social rank or other potentially confounding factors. Considering the phylogenetic positions of F. mechowii and F. anselli and unpublished data on a third Fukomys-species (F. damarensis showing essentially the same pattern, it seems probable that the reversal of the classic trade-off between somatic maintenance and sexual reproduction is characteristic of the whole genus and hence of the vast majority of social mole-rats.

  11. The eye of the African mole-rat Cryptomys anselli : to see or not to see?

    NARCIS (Netherlands)

    Cernuda-Cernuda, R; Garcia-Fernandez, JM; Gordijn, MCM; Bovee-Geurts, PHM; DeGrip, WJ; de Grip, W.J.

    2003-01-01

    In an attempt to clarify its possible physiological role, we studied the eye of the Zambian mole rat Cryptomys anselli by light, electron and confocal microscopy using conventional staining as well as immunolabelling with rod and cone cell markers. The small eyes of Cryptomys are located superficial

  12. Family Wide Molecular Adaptations to Underground Life in African Mole-Rats Revealed by Phylogenomic Analysis.

    Science.gov (United States)

    Davies, Kalina T J; Bennett, Nigel C; Tsagkogeorga, Georgia; Rossiter, Stephen J; Faulkes, Christopher G

    2015-12-01

    During their evolutionary radiation, mammals have colonized diverse habitats. Arguably the subterranean niche is the most inhospitable of these, characterized by reduced oxygen, elevated carbon dioxide, absence of light, scarcity of food, and a substrate that is energetically costly to burrow through. Of all lineages to have transitioned to a subterranean niche, African mole-rats are one of the most successful. Much of their ecological success can be attributed to a diet of plant storage organs, which has allowed them to colonize climatically varied habitats across sub-Saharan Africa, and has probably contributed to the evolution of their diverse social systems. Yet despite their many remarkable phenotypic specializations, little is known about molecular adaptations underlying these traits. To address this, we sequenced the transcriptomes of seven mole-rat taxa, including three solitary species, and combined new sequences with existing genomic data sets. Alignments of more than 13,000 protein-coding genes encompassed, for the first time, all six genera and the full spectrum of ecological and social variation in the clade. We detected positive selection within the mole-rat clade and along ancestral branches in approximately 700 genes including loci associated with tumorigenesis, aging, morphological development, and sociality. By combining these results with gene ontology annotation and protein-protein networks, we identified several clusters of functionally related genes. This family wide analysis of molecular evolution in mole-rats has identified a suite of positively selected genes, deepening our understanding of the extreme phenotypic traits exhibited by this group.

  13. Effects of ultraviolet radiation on mole rats kidney: A histopathologic and ultrastructural study

    Directory of Open Access Journals (Sweden)

    Hüseyin Türker

    2014-04-01

    Full Text Available The purpose of this study was to realize the ultrastructural effects of ultraviolet radiation on the kidney tissue cells of mole rats (Spalax leucodon. The mole rats of 180–200 g body weight were divided into the control and radiation-trial groups. The control group was not given any radiation. The other groups were irradiated with artificially produced UVC radiation for 14, 28 and 60 days. The kidney tissue samples were prepared at the end of experiments and analyzed by the light and electron microscope. Several effects were observed in the kidney tissues cells analyzed in accordance with the dose magnitude of radiation. These results clearly show the detrimental effects of UVC radiation on kidney tissue cells in exposure periods dependent on radiation dose and exposure time.

  14. Seasonal changes in burrow geometry of the common mole rat (Rodentia: Bathyergidae)

    Science.gov (United States)

    Thomas, H. G.; Scantlebury, M.; Swanepoel, D.; Bateman, P. W.; Bennett, N. C.

    2013-11-01

    Sociality in mole rats has been suggested to have evolved as a response to the widely dispersed food resources and the limited burrowing opportunities that result from sporadic rainfall events. In the most arid regions, individual foraging efficiency is reduced, and energetic constraints increase. In this study, we investigate seasonal differences in burrow architecture of the social Cryptomys hottentotus hottentotus in a mesic region. We describe burrow geometry in response to seasonal weather conditions for two seasons (wet and dry). Interactions occurred between seasons and colony size for the size of the burrow systems, but not the shape of the burrow systems. The fractal dimension values of the burrow systems did not differ between seasons. Thus, the burrow complexity was dependent upon the number of mole rats present in the social group.

  15. Genetic signatures for enhanced olfaction in the African mole-rats.

    Directory of Open Access Journals (Sweden)

    Sofia Stathopoulos

    Full Text Available The Olfactory Receptor (OR superfamily, the largest in the vertebrate genome, is responsible for vertebrate olfaction and is traditionally subdivided into 17 OR families. Recent studies characterising whole-OR subgenomes revealed a 'birth and death' model of evolution for a range of species, however little is known about fine-scale evolutionary dynamics within single-OR families. This study reports the first assessment of fine-scale OR evolution and variation in African mole-rats (Bathyergidae, a family of subterranean rodents endemic to sub-Saharan Africa. Because of the selective pressures of life underground, enhanced olfaction is proposed to be fundamental to the evolutionary success of the Bathyergidae, resulting in a highly diversified OR gene-repertoire. Using a PCR-sequencing approach, we analysed variation in the OR7 family across 14 extant bathyergid species, which revealed enhanced levels of functional polymorphisms concentrated across the receptors' ligand-binding region. We propose that mole-rats are able to recognise a broad range of odorants and that this diversity is reflected throughout their OR7 gene repertoire. Using both classic tests and tree-based methods to test for signals of selection, we investigate evolutionary forces across the mole-rat OR7 gene tree. Four well-supported clades emerged in the OR phylogeny, with varying signals of selection; from neutrality to positive and purifying selection. Bathyergid life-history traits and environmental niche-specialisation are explored as possible drivers of adaptive OR evolution, emerging as non-exclusive contributors to the positive selection observed at OR7 genes. Our results reveal unexpected complexity of evolutionary mechanisms acting within a single OR family, providing insightful perspectives into OR evolutionary dynamics.

  16. Mechanisms regulating proteostasis are involved in sympatric speciation of the blind mole rat, Spalax galili.

    Science.gov (United States)

    Rodriguez, Karl A; Li, Kexin; Nevo, Eviatar; Buffenstein, Rochelle

    2016-01-01

    Genome-wide analysis demonstrates extensive genomic adaptive complexes involved in sympatric speciation between blind mole rats (Spalax galili) in abutting populations living in basalt and chalk soils. Among the gene ontology (GO) enrichment, musculature and metabolism stood out in basalt dwellers while nutrition and neurogenetics were highlighted in chalk residents. Measurements of mechanisms regulating protein homeostasis inspired by these GO terms suggest that at the proteomic level there is also a habitat/soil-type driven divergence with the basalt residents exhibiting higher proteasome activity whereas elevated levels of markers of autophagy are evident in the chalk inhabitants.

  17. Transcriptome, genetic editing, and microRNA divergence substantiate sympatric speciation of blind mole rat, Spalax.

    Science.gov (United States)

    Li, Kexin; Wang, Liuyang; Knisbacher, Binyamin A; Xu, Qinqin; Levanon, Erez Y; Wang, Huihua; Frenkel-Morgenstern, Milana; Tagore, Satabdi; Fang, Xiaodong; Bazak, Lily; Buchumenski, Ilana; Zhao, Yang; Lövy, Matěj; Li, Xiangfeng; Han, Lijuan; Frenkel, Zeev; Beiles, Avigdor; Cao, Yi Bin; Wang, Zhen Long; Nevo, Eviatar

    2016-07-05

    Incipient sympatric speciation in blind mole rat, Spalax galili, in Israel, caused by sharp ecological divergence of abutting chalk-basalt ecologies, has been proposed previously based on mitochondrial and whole-genome nuclear DNA. Here, we present new evidence, including transcriptome, DNA editing, microRNA, and codon usage, substantiating earlier evidence for adaptive divergence in the abutting chalk and basalt populations. Genetic divergence, based on the previous and new evidence, is ongoing despite restricted gene flow between the two populations. The principal component analysis, neighbor-joining tree, and genetic structure analysis of the transcriptome clearly show the clustered divergent two mole rat populations. Gene-expression level analysis indicates that the population transcriptome divergence is displayed not only by soil divergence but also by sex. Gene ontology enrichment of the differentially expressed genes from the two abutting soil populations highlights reproductive isolation. Alternative splicing variation of the two abutting soil populations displays two distinct splicing patterns. L-shaped FST distribution indicates that the two populations have undergone divergence with gene flow. Transcriptome divergent genes highlight neurogenetics and nutrition characterizing the chalk population, and energetics, metabolism, musculature, and sensory perception characterizing the abutting basalt population. Remarkably, microRNAs also display divergence between the two populations. The GC content is significantly higher in chalk than in basalt, and stress-response genes mostly prefer nonoptimal codons. The multiple lines of evidence of ecological-genomic and genetic divergence highlight that natural selection overrules the gene flow between the two abutting populations, substantiating the sharp ecological chalk-basalt divergence driving sympatric speciation.

  18. Lipid profile and serum characteristics of the blind subterranean mole rat, Spalax.

    Directory of Open Access Journals (Sweden)

    Nicola J Nasser

    Full Text Available BACKGROUND: Spalax (blind subterranean mole rat, is a mammal adapted to live in fluctuating oxygen levels, and can survive severe hypoxia and hypercapnia. The adaptive evolution of Spalax to underground life resulted in structural and molecular-genetic differences comparing to above-ground mammals. These differences include higher myocardial maximal oxygen consumption, increased lung diffusion capacity, increased blood vessels density, and unique expression patterns of cancer and angiogenesis related genes such as heparanase, vascular endothelial growth factor, and P53. METHODOLOGY/PRINCIPAL FINDINGS: Here we elucidate the main characteristics of Spalax lipid profile, as well as its main antioxidant and serum parameters. Compared to human, Spalax possesses lower total-cholesterol, low density lipoproteins (LDL and triglycerides levels, and higher levels of high density lipoproteins (HDL. Apolipoprotein A-I and apolipoprotein B-100 were significantly lower in Spalax compared to human. Paraoxonase (PON 1 arylesterase activity, was higher in Spalax compared to both human and mouse serum levels. Analysis of serum chemistry of Spalax revealed special features in this mammal. CONCLUSIONS/SIGNIFICANCE: Spalax possesses a unique lipid profile with high HDL and low LDL lipoproteins. The antioxidant serum content in the mole rat is higher than that of human and mouse. Serum C reactive protein (CRP levels are significantly lower in Spalax compared to that of human or mouse, reflecting low levels of inflammation. These differences between Spalax, human and mouse are due to several factors including the intensive activity life-style that Spalax pursue underground, dietary components, and evolutionary genetic adaptations. Unfolding the genetic basis of these differences will probably result in unique treatments for a variety of human diseases such as dyslipedemias, inflammation and cancer.

  19. Naked Firewalls

    CERN Document Server

    Chen, Pisin; Page, Don N; Sasaki, Misao; Yeom, Dong-han

    2015-01-01

    In the firewall proposal, it is assumed that the firewall lies near the event horizon and should not be observable except by infalling observers, who are presumably terminated at the firewall. However, if the firewall is located near where the horizon would have been, based on the spacetime evolution up to that time, later quantum fluctuations of the Hawking emission rate can cause the "teleological" event horizon to have migrated to the inside of the firewall location, rendering the firewall naked. In principle, the firewall can be arbitrarily far outside the horizon. This casts doubt about the notion that firewalls are the "most conservative" solution to the information loss paradox.

  20. Adaptive methylation regulation of p53 pathway in sympatric speciation of blind mole rats, Spalax.

    Science.gov (United States)

    Zhao, Yang; Tang, Jia-Wei; Yang, Zhi; Cao, Yi-Bin; Ren, Ji-Long; Ben-Abu, Yuval; Li, Kexin; Chen, Xue-Qun; Du, Ji-Zeng; Nevo, Eviatar

    2016-02-23

    Epigenetic modifications play significant roles in adaptive evolution. The tumor suppressor p53, well known for controlling cell fate and maintaining genomic stability, is much less known as a master gene in environmental adaptation involving methylation modifications. The blind subterranean mole rat Spalax eherenbergi superspecies in Israel consists of four species that speciated peripatrically. Remarkably, the northern Galilee species Spalax galili (2n = 52) underwent adaptive ecological sympatric speciation, caused by the sharply divergent chalk and basalt ecologies. This was demonstrated by mitochondrial and nuclear genomic evidence. Here we show that the expression patterns of the p53 regulatory pathway diversified between the abutting sympatric populations of S. galili in sharply divergent chalk-basalt ecologies. We identified higher methylation on several sites of the p53 promoter in the population living in chalk soil (chalk population). Site mutagenesis showed that methylation on these sites linked to the transcriptional repression of p53 involving Cut-Like Homeobox 1 (Cux1), paired box 4 (Pax 4), Pax 6, and activator protein 1 (AP-1). Diverse expression levels of p53 between the incipiently sympatrically speciating chalk-basalt abutting populations of S. galili selectively affected cell-cycle arrest but not apoptosis. We hypothesize that methylation modification of p53 has adaptively shifted in supervising its target genes during sympatric speciation of S. galili to cope with the contrasting environmental stresses of the abutting divergent chalk-basalt ecologies.

  1. Macroscopic anatomy of the lower respiratory system in mole rats (Spalax leucodon).

    Science.gov (United States)

    İlgun, R; Yoldas, A; Kuru, N; Özkan, Z E

    2014-12-01

    The morphologic and morphometric features of the lower respiratory system in mole rats were examined. It was seen that the low respiratory system of this species leading a special life under highly hypoxic/hypercapnic conditions underground is structurally similar to other mammals living on land in terms of the parts examined; trachea was formed by 29.5 ± 4 oval-formed cartilaginous tracheals arranged backwards and became gradually more stenotic diameter from cranial to the caudal of the neck. The trachea was separated in two principal bronchus at the fourth thoracal intercostal spatium level. The angle between the two main principal bronchi was 60.5 ± 2.35°. The lung constituted 1.29 ± 0.03% of the body weight and the right lung was heavier than the left lung. Fissura inter-lobaris was deep and separated the lung lobes wholly, and the right lung was separated in four lobes, whereas the left lung was not separated into the lobes. Also, the medial lobe of the left lung was the lightest lobe.

  2. Effects of laboratory housing on exploratory behaviour, novelty discrimination and spatial reference memory in a subterranean, solitary rodent, the Cape mole-rat (Georychus capensis)

    OpenAIRE

    Oosthuizen, Maria Kathleen; Scheibler, Anne-Gita; Bennett, Nigel Charles; Amrein, Irmgard

    2013-01-01

    A large number of laboratory and field based studies are being carried out on mole-rats, both in our research group and others. Several studies have highlighted the development of adverse behaviours in laboratory animals and have emphasised the importance of enrichment for captive animals. Hence we were interested in evaluating how laboratory housing would affect behavioural performance in mole-rats. We investigated exploratory behaviour, the ability to discriminate between novel and familiar...

  3. Local and regional scale genetic variation in the Cape dune mole-rat, Bathyergus suillus.

    Science.gov (United States)

    Visser, Jacobus H; Bennett, Nigel C; Jansen van Vuuren, Bettine

    2014-01-01

    The distribution of genetic variation is determined through the interaction of life history, morphology and habitat specificity of a species in conjunction with landscape structure. While numerous studies have investigated this interplay of factors in species inhabiting aquatic, riverine, terrestrial, arboreal and saxicolous systems, the fossorial system has remained largely unexplored. In this study we attempt to elucidate the impacts of a subterranean lifestyle coupled with a heterogeneous landscape on genetic partitioning by using a subterranean mammal species, the Cape dune mole-rat (Bathyergus suillus), as our model. Bathyergus suillus is one of a few mammal species endemic to the Cape Floristic Region (CFR) of the Western Cape of South Africa. Its distribution is fragmented by rivers and mountains; both geographic phenomena that may act as geographical barriers to gene-flow. Using two mitochondrial fragments (cytochrome b and control region) as well as nine microsatellite loci, we determined the phylogeographic structure and gene-flow patterns at two different spatial scales (local and regional). Furthermore, we investigated genetic differentiation between populations and applied Bayesian clustering and assignment approaches to our data. Nearly every population formed a genetically unique entity with significant genetic structure evident across geographic barriers such as rivers (Berg, Verlorenvlei, Breede and Gourits Rivers), mountains (Piketberg and Hottentots Holland Mountains) and with geographic distance at both spatial scales. Surprisingly, B. suillus was found to be paraphyletic with respect to its sister species, B. janetta-a result largely overlooked by previous studies on these taxa. A systematic revision of the genus Bathyergus is therefore necessary. This study provides a valuable insight into how the biology, life-history and habitat specificity of animals inhabiting a fossorial system may act in concert with the structure of the surrounding

  4. Teasing apart socially-induced infertility in non-reproductive female Damaraland mole-rats, Fukomys damarensis (Rodentia: Bathyergidae).

    Science.gov (United States)

    Bennett, Nigel C

    2011-12-01

    The Damaraland mole-rat is a subterranean mammal exhibiting extreme reproductive skew with a single reproductive female in each colony responsible for procreation. Non-reproductive female colony members are physiologically suppressed while in the colony, exhibiting reduced concentrations of plasma luteinizing hormone (LH) and a decreased response of the pituitary, as measured by the release of bioactive LH, to an exogenous dose of gonadotrophin releasing hormone (GnRH). Removal of the reproductive female from the colony results in an elevation of LH and an enhanced response of the pituitary to a GnRH challenge in non-reproductive females comparable to reproductive females, implying control of reproduction in these individuals by the reproductive female. The Damaraland mole-rat is an ideal model for investigating the physiological and behavioral mechanisms that regulate the hypothalamo-pituitary-gonadal axis. In contrast, we know less about the control of reproduction at the level of the hypothalamus. The immunohistochemistry of the GnRH system of both reproductive and non-reproductive female Damaraland mole-rats has revealed no significant differences with respect to morphology, distribution or numbers of immunoreactive GnRH perikarya. We examined whether the endogenous opioid peptide beta-endorphin was responsible for the inhibition of the release of the GnRH from the neurons indirectly by measuring LH concentrations in these non-reproductive females following single, hourly and 8 hourly injections of the opioid antagonist naloxone. The results imply that the endogenous opioid peptide, beta-endorphin, is not responsible for the inhibition of GnRH release from the perikarya in non-reproductive females. Preliminary data examining the circulating levels of cortisol also do not support a role for circulating glucocorticoids. The possible role of kisspeptin is discussed.

  5. Gis-approach for variability assessment of soil electric conductivity under pedoturbation activity of mole rat (Spalax microphthalmus

    Directory of Open Access Journals (Sweden)

    T. М. Konovalova

    2010-06-01

    Full Text Available The results of the investigation of the impact of the mole rat’s activity on soil electric conductivity have been presented. GIS-technology have been shown to be effective for assessment of the pedoturbation activity effect on the soil surface heterogeneity formation. Method of the one-dimension spatial coordinated array transformation into matrix form has been proposed for following multidimension statistic analysis application. The quantity estimation of the mole rats role in formation of the habitat nanorelief-level diversity has been obtained by means of indexes of the landscape complexity and diversity.

  6. Transcriptome sequencing of the blind subterranean mole rat, Spalax galili: Utility and potential for the discovery of novel evolutionary patterns

    KAUST Repository

    Malik, Assaf

    2011-08-12

    The blind subterranean mole rat (Spalax ehrenbergi superspecies) is a model animal for survival under extreme environments due to its ability to live in underground habitats under severe hypoxic stress and darkness. Here we report the transcriptome sequencing of Spalax galili, a chromosomal type of S. ehrenbergi. cDNA pools from muscle and brain tissues isolated from animals exposed to hypoxic and normoxic conditions were sequenced using Sanger, GS FLX, and GS FLX Titanium technologies. Assembly of the sequences yielded over 51,000 isotigs with homology to ~12,000 mouse, rat or human genes. Based on these results, it was possible to detect large numbers of splice variants, SNPs, and novel transcribed regions. In addition, multiple differential expression patterns were detected between tissues and treatments. The results presented here will serve as a valuable resource for future studies aimed at identifying genes and gene regions evolved during the adaptive radiation associated with underground life of the blind mole rat. 2011 Malik et al.

  7. Transcriptome sequencing of the blind subterranean mole rat, Spalax galili: utility and potential for the discovery of novel evolutionary patterns.

    Directory of Open Access Journals (Sweden)

    Assaf Malik

    Full Text Available The blind subterranean mole rat (Spalax ehrenbergi superspecies is a model animal for survival under extreme environments due to its ability to live in underground habitats under severe hypoxic stress and darkness. Here we report the transcriptome sequencing of Spalax galili, a chromosomal type of S. ehrenbergi. cDNA pools from muscle and brain tissues isolated from animals exposed to hypoxic and normoxic conditions were sequenced using Sanger, GS FLX, and GS FLX Titanium technologies. Assembly of the sequences yielded over 51,000 isotigs with homology to ∼12,000 mouse, rat or human genes. Based on these results, it was possible to detect large numbers of splice variants, SNPs, and novel transcribed regions. In addition, multiple differential expression patterns were detected between tissues and treatments. The results presented here will serve as a valuable resource for future studies aimed at identifying genes and gene regions evolved during the adaptive radiation associated with underground life of the blind mole rat.

  8. Spatial and temporal activity patterns of the free-living giant mole-rat (Fukomys mechowii, the largest social bathyergid.

    Directory of Open Access Journals (Sweden)

    Matěj Lövy

    Full Text Available Despite the considerable attention devoted to the biology of social species of African mole-rats (Bathyergidae, Rodentia, knowledge is lacking about their behaviour under natural conditions. We studied activity of the largest social bathyergid, the giant mole-rat Fukomys mechowii, in its natural habitat in Zambia using radio-telemetry. We radio-tracked six individuals during three continuous 72-h sessions. Five of these individuals, including a breeding male, belonged to a single family group; the remaining female was probably a solitary disperser. The non-breeders of the family were active (i.e. outside the nest 5.8 hours per 24h-day with the activity split into 6.5 short bouts. The activity was more concentrated in the night hours, when the animals also travelled longer distances from the nest. The breeding male spent only 3.2 hours per day outside the nest, utilizing less than 20% of the whole family home range. The dispersing female displayed a much different activity pattern than the family members. Her 8.0 hours of outside-nest activity per day were split into 4.6 bouts which were twice as long as in the family non-breeders. Her activity peak in the late afternoon coincided with the temperature maximum in the depth of 10 cm (roughly the depth of the foraging tunnels. Our results suggest that the breeding individuals (at least males contribute very little to the work of the family group. Nevertheless, the amount of an individual's activity and its daily pattern are probably flexible in this species and can be modified in response to actual environmental and social conditions.

  9. Size variation in Tachyoryctes splendens (East African mole-rat) and its implications for late Quaternary temperature change in equatorial East Africa

    Science.gov (United States)

    Faith, J. Tyler; Patterson, David B.; Blegen, Nick; O'Neill, Chris J.; Marean, Curtis W.; Peppe, Daniel J.; Tryon, Christian A.

    2016-05-01

    This study develops a new proxy for Quaternary temperature change in tropical Africa through analysis of size variation in East African mole-rat (Tachyoryctes splendens). In modern mole-rats, mandibular alveolar length is unrelated to annual precipitation, precipitation seasonality, temperature seasonality, or primary productivity. However, it is inversely correlated with mean annual temperature, in agreement with Bergmann's rule. This relationship is observed at temperatures below ˜17.3 °C, but not at higher temperatures. We apply these observations to late Quaternary mole-rats from Wakondo (˜100 ka) and Kisaaka (˜50 ka) in the Lake Victoria region and Enkapune ya Muto (EYM; ˜7.2-3.2 ka) in Kenya's central rift. The Lake Victoria mole-rats are larger than expected for populations from warm climates typical of the area today, implying cooler temperatures in the past. The magnitude of temperature decline needed to drive the size shift is substantial (˜4-6 °C), similar in magnitude to the degree of change between the Last Glacial Maximum and Holocene, but is consistent with regional temperature records and with scenarios linking equatorial African temperature to northern hemisphere summer insolation. Size changes through time at EYM indicate that rising temperatures during the middle Holocene accompanied and potentially contributed to a decline in Lake Naivasha and expansion of grassland vegetation.

  10. Non-image Forming Light Detection by Melanopsin, Rhodopsin, and Long-Middlewave (L/W) Cone Opsin in the Subterranean Blind Mole Rat, Spalax Ehrenbergi

    DEFF Research Database (Denmark)

    Esquiva, Gema; Avivi, Aaron; Hannibal, Jens

    2016-01-01

    The blind mole rat, Spalax ehrenbergi, can, despite severely degenerated eyes covered by fur, entrain to the daily light/dark cycle and adapt to seasonal changes due to an intact circadian timing system. The present study demonstrates that the Spalax retina contains a photoreceptor layer, an outer...

  11. Effects of laboratory housing on exploratory behaviour, novelty discrimination and spatial reference memory in a subterranean, solitary rodent, the Cape mole-rat (Georychus capensis).

    Science.gov (United States)

    Oosthuizen, Maria Kathleen; Scheibler, Anne-Gita; Bennett, Nigel Charles; Amrein, Irmgard

    2013-01-01

    A large number of laboratory and field based studies are being carried out on mole-rats, both in our research group and others. Several studies have highlighted the development of adverse behaviours in laboratory animals and have emphasised the importance of enrichment for captive animals. Hence we were interested in evaluating how laboratory housing would affect behavioural performance in mole-rats. We investigated exploratory behaviour, the ability to discriminate between novel and familiar environments and reference memory in the solitary Cape mole-rat (Georychus capensis). Our data showed that both wild and captive animals readily explore open spaces and tunnels. Wild animals were however more active than their captive counterparts. In the Y maze two trial discrimination task, wild animals failed to discriminate between novel and familiar environments, while laboratory housed mole-rats showed preferential spatial discrimination in terms of the length of time spent in the novel arm. The performance of the laboratory and wild animals were similar when tested for reference memory in the Y maze, both groups showed a significant improvement compared to the first day, from the 3rd day onwards. Wild animals made more mistakes whereas laboratory animals were slower in completing the task. The difference in performance between wild and laboratory animals in the Y-maze may be as a result of the lower activity of the laboratory animals. Laboratory maintained Cape mole-rats show classic behaviours resulting from a lack of stimulation such as reduced activity and increased aggression. However, they do display an improved novelty discrimination compared to the wild animals. Slower locomotion rate of the laboratory animals may increase the integration time of stimuli, hence result in a more thorough inspection of the surroundings. Unlike the captive animals, wild animals show flexibility in their responses to unpredictable events, which is an important requirement under

  12. Dental peculiarities in the silvery mole-rat: an original model for studying the evolutionary and biological origins of continuous dental generation in mammals

    Directory of Open Access Journals (Sweden)

    Helder Gomes Rodrigues

    2015-09-01

    Full Text Available Unravelling the evolutionary and developmental mechanisms that have impacted the mammalian dentition, since more than 200 Ma, is an intricate issue. Interestingly, a few mammal species, including the silvery mole-rat Heliophobius argenteocinereus, are able to replace their dentition by the addition of supernumerary molars at the back of jaw migrating then toward the front. The aim here was to demonstrate the potential interest of further studying this rodent in order to better understand the origins of continuous dental replacement in mammals, which could also provide interesting data concerning the evolution of limited dental generation occurring in first mammals. In the present study, we described the main stages of the dental eruptive sequence in the silvery mole-rat and the associated characteristics of horizontal replacement using X-ray microtomography. This was coupled to the investigation of other African mole-rats which have no dental replacement. This method permitted to establish evidence that the initial development of the dentition in Heliophobius is comparable to what it is observed in most of African mole-rats. This rodent first has premolars, but then identical additional molars, a mechanism convergent to manatees and the pygmy rock-wallaby. Evidence of continuous replacement and strong dental dynamics were also illustrated in Heliophobius, and stressed the need to deeply investigate these aspects for evolutionary, functional and developmental purposes. We also noticed that two groups of extinct non-mammalian synapsids convergently acquired this dental mechanism, but in a way differing from extant mammals. The discussion on the diverse evolutionary origins of horizontal dental replacement put emphasis on the necessity of focusing on biological parameters potentially involved in both continuous and limited developments of teeth in mammals. In that context, the silvery mole-rat could appear as the most appropriate candidate to do so.

  13. Circadian genes in a blind subterranean mammal II: Conservation and uniqueness of the three Period homologs in the blind subterranean mole rat, Spalax ehrenbergi superspecies

    OpenAIRE

    Avivi, A; Oster, H; A Joel; BEILES, A.; Albrecht, U; Nevo, E.

    2002-01-01

    We demonstrated that a subterranean, visually blind mammal has a functional set of three Per genes that are important components of the circadian clockwork in mammals. The mole rat superspecies Spalax ehrenbergi is a blind subterranean animal that lives its entire life underground in darkness. It has degenerated eyes, but the retina and highly hypertrophic harderian gland are involved in photoperiodic perception. All three Per genes oscillate with a periodicity of 24 h in the suprachiasmatic ...

  14. Non-Breeding Eusocial Mole-Rats Produce Viable Sperm--Spermiogram and Functional Testicular Morphology of Fukomys anselli.

    Directory of Open Access Journals (Sweden)

    Angelica Garcia Montero

    Full Text Available Ansell's mole-rats (Fukomys anselli are subterranean rodents living in families composed of about 20 members with a single breeding pair and their non-breeding offspring. Most of them remain with their parents for their lifetime and help to maintain and defend the natal burrow system, forage, and care for younger siblings. Since incest avoidance is based on individual recognition (and not on social suppression we expect that non-breeders produce viable sperm spontaneously. We compared the sperm of breeding and non-breeding males, obtained by electroejaculation and found no significant differences in sperm parameters between both groups. Here, we used electroejaculation to obtain semen for the first time in a subterranean mammal. Spermiogram analysis revealed no significant differences in sperm parameters between breeders and non-breeders. We found significantly larger testes (measured on autopsies and on living animals per ultrasonography of breeders compared to non-breeders (with body mass having a significant effect. There were no marked histological differences between breeding and non-breeding males, and the relative area occupied by Leydig cells and seminiferous tubules on histological sections, respectively, was not significantly different between both groups. The seminiferous epithelium and to a lesser degree the interstitial testicular tissue are characterized by lesions (vacuolar degenerations, however, this feature does not hinder fertilization even in advanced stages of life. The continuous production of viable sperm also in sexually abstinent non-breeders might be best understood in light of the mating and social system of Fukomys anselli, and the potential to found a new family following an unpredictable and rare encounter with an unfamiliar female ("provoked or induced dispersal". Apparently, the non-breeders do not reproduce because they do not copulate but not because they would be physiologically infertile. The significantly

  15. Non-Breeding Eusocial Mole-Rats Produce Viable Sperm—Spermiogram and Functional Testicular Morphology of Fukomys anselli

    Science.gov (United States)

    Garcia Montero, Angelica; Vole, Christiane; Burda, Hynek; Malkemper, Erich Pascal; Holtze, Susanne; Morhart, Michaela; Saragusty, Joseph; Hildebrandt, Thomas B.; Begall, Sabine

    2016-01-01

    Ansell’s mole-rats (Fukomys anselli) are subterranean rodents living in families composed of about 20 members with a single breeding pair and their non-breeding offspring. Most of them remain with their parents for their lifetime and help to maintain and defend the natal burrow system, forage, and care for younger siblings. Since incest avoidance is based on individual recognition (and not on social suppression) we expect that non-breeders produce viable sperm spontaneously. We compared the sperm of breeding and non-breeding males, obtained by electroejaculation and found no significant differences in sperm parameters between both groups. Here, we used electroejaculation to obtain semen for the first time in a subterranean mammal. Spermiogram analysis revealed no significant differences in sperm parameters between breeders and non-breeders. We found significantly larger testes (measured on autopsies and on living animals per ultrasonography) of breeders compared to non-breeders (with body mass having a significant effect). There were no marked histological differences between breeding and non-breeding males, and the relative area occupied by Leydig cells and seminiferous tubules on histological sections, respectively, was not significantly different between both groups. The seminiferous epithelium and to a lesser degree the interstitial testicular tissue are characterized by lesions (vacuolar degenerations), however, this feature does not hinder fertilization even in advanced stages of life. The continuous production of viable sperm also in sexually abstinent non-breeders might be best understood in light of the mating and social system of Fukomys anselli, and the potential to found a new family following an unpredictable and rare encounter with an unfamiliar female (“provoked or induced dispersal”). Apparently, the non-breeders do not reproduce because they do not copulate but not because they would be physiologically infertile. The significantly increased

  16. Non-Breeding Eusocial Mole-Rats Produce Viable Sperm--Spermiogram and Functional Testicular Morphology of Fukomys anselli.

    Science.gov (United States)

    Garcia Montero, Angelica; Vole, Christiane; Burda, Hynek; Malkemper, Erich Pascal; Holtze, Susanne; Morhart, Michaela; Saragusty, Joseph; Hildebrandt, Thomas B; Begall, Sabine

    2016-01-01

    Ansell's mole-rats (Fukomys anselli) are subterranean rodents living in families composed of about 20 members with a single breeding pair and their non-breeding offspring. Most of them remain with their parents for their lifetime and help to maintain and defend the natal burrow system, forage, and care for younger siblings. Since incest avoidance is based on individual recognition (and not on social suppression) we expect that non-breeders produce viable sperm spontaneously. We compared the sperm of breeding and non-breeding males, obtained by electroejaculation and found no significant differences in sperm parameters between both groups. Here, we used electroejaculation to obtain semen for the first time in a subterranean mammal. Spermiogram analysis revealed no significant differences in sperm parameters between breeders and non-breeders. We found significantly larger testes (measured on autopsies and on living animals per ultrasonography) of breeders compared to non-breeders (with body mass having a significant effect). There were no marked histological differences between breeding and non-breeding males, and the relative area occupied by Leydig cells and seminiferous tubules on histological sections, respectively, was not significantly different between both groups. The seminiferous epithelium and to a lesser degree the interstitial testicular tissue are characterized by lesions (vacuolar degenerations), however, this feature does not hinder fertilization even in advanced stages of life. The continuous production of viable sperm also in sexually abstinent non-breeders might be best understood in light of the mating and social system of Fukomys anselli, and the potential to found a new family following an unpredictable and rare encounter with an unfamiliar female ("provoked or induced dispersal"). Apparently, the non-breeders do not reproduce because they do not copulate but not because they would be physiologically infertile. The significantly increased testes

  17. Relic populations of Fukomys mole-rats in Tanzania: description of two new species F. livingstoni sp. nov. and F. hanangensis sp. nov.

    Directory of Open Access Journals (Sweden)

    Chris G. Faulkes

    2017-04-01

    Full Text Available Previous studies of African mole-rats of the genera Heliophobius and Fukomys (Bathyergidae in the regions of East and south central Africa have revealed a diversity of species and vicariant populations, with patterns of distribution having been influenced by the geological process of rifting and changing patterns of drainage of major river systems. This has resulted in most of the extant members of the genus Fukomys being distributed west of the main Rift Valley. However, a small number of isolated populations are known to occur east of the African Rift Valley in Tanzania, where Heliophobius is the most common bathyergid rodent. We conducted morphological, craniometric and phylogenetic analysis of mitochondrial cytochrome b (cyt b sequences of two allopatric populations of Tanzanian mole-rats (genus Fukomys at Ujiji and around Mount Hanang, in comparison with both geographically adjacent and more distant populations of Fukomys. Our results reveal two distinct evolutionary lineages, forming clades that constitute previously unnamed species. Here, we formally describe and designate these new species F. livingstoni and F. hanangensis respectively. Molecular clock-based estimates of divergence times, together with maximum likelihood inference of biogeographic range evolution, offers strong support for the hypothesis that vicariance in the Western Rift Valley and the drainage patterns of major river systems has subdivided populations of mole-rats. More recent climatic changes and tectonic activity in the “Mbeya triple junction” and Rungwe volcanic province between Lakes Rukwa and Nyasa have played a role in further isolation of these extra-limital populations of Fukomys in Tanzania.

  18. Do naked singularities form?

    CERN Document Server

    Vaz, C; Vaz, Cenalo; Witten, Louis

    1995-01-01

    A naked singularity is formed by the collapse of a Sine-Gordon soliton in 1+1 dimensional dilaton gravity with a negative cosmological constant. We examine the quantum stress tensor resulting from the formation of the singularity. Consistent boundary conditions require that the incoming soliton is accompanied by a flux of incoming radiation across past null infinity, but neglecting the back reaction of the spacetime leads to the absurd conclusion that the total energy entering the system by the time the observer is able to receive information from the singularity is infinite. We conclude that the back reaction must prevent the formation of the naked singularity.

  19. Neuroanatomical investigation of the gonadotrophin-releasing hormone 1 system in the seasonally breeding Cape dune mole-rat, Bathyergus suillus.

    Science.gov (United States)

    Hart, Leanne; Bennett, Nigel C; Kalamatianos, Theodosis; Oosthuizen, Maria K; Jarvis, Jennifer U M; O'Riain, M Justin; Coen, Clive W

    2008-10-22

    The gonadotrophin-releasing hormone 1 (GnRH1) system has been investigated immunohistochemically in Cape dune mole-rats (Bathyergus suillus), subterranean rodents that normally display severe aggression towards conspecifics. These animals breed seasonally and show a reduced mean plasma level of luteinising hormone during the non-breeding season. GnRH1-immunoreactive (ir) cell bodies and processes are found in the septal/preoptic area and the mediobasal hypothalamus; the cell bodies are found in equal measure in these two regions. Dense aggregations of GnRH1-ir fibres are present in the organum vasculosum of the lamina terminalis and the external zone of the median eminence. The total number of detectable GnRH1-ir cell bodies does not differ between the sexes or within the sexes between breeding and non-breeding seasons. Similarly there is no difference in the distribution of detectable GnRH1-ir cell bodies in male and female mole-rats in and out of the breeding season. Although the average size of GnRH1-ir cell bodies does not differ between the seasons in males, their size in females is significantly smaller in the non-breeding season. Whether this reduced size reflects reduced GnRH1 synthesis remains to be determined.

  20. Reproduction is associated with a tissue-dependent reduction of oxidative stress in eusocial female Damaraland mole-rats (Fukomys damarensis.

    Directory of Open Access Journals (Sweden)

    Christina M Schmidt

    Full Text Available Oxidative stress has been implicated as both a physiological cost of reproduction and a driving force on an animal's lifespan. Since increased reproductive effort is generally linked with a reduction in survival, it has been proposed that oxidative stress may influence this relationship. Support for this hypothesis is inconsistent, but this may, in part, be due to the type of tissues that have been analyzed. In Damaraland mole-rats the sole reproducing female in the colony is also the longest lived. Therefore, if oxidative stress does impact the trade-off between reproduction and survival in general, this species may possess some form of enhanced defense. We assessed this relationship by comparing markers of oxidative damage (malondialdehyde, MDA; protein carbonyls, PC and antioxidants (total antioxidant capacity, TAC; superoxide dismutase, SOD in various tissues including plasma, erythrocytes, heart, liver, kidney and skeletal muscle between wild-caught reproductive and non-reproductive female Damaraland mole-rats. Reproductive females exhibited significantly lower levels of PC across all tissues, and lower levels of MDA in heart, kidney and liver relative to non-reproductive females. Levels of TAC and SOD did not differ significantly according to reproductive state. The reduction in oxidative damage in breeding females may be attributable to the unusual social structure of this species, as similar relationships have been observed between reproductive and non-reproductive eusocial insects.

  1. How attractive is the girl next door? An assessment of spatial mate acquisition and paternity in the solitary Cape dune mole-rat, Bathyergus suillus.

    Directory of Open Access Journals (Sweden)

    Timothy C Bray

    Full Text Available Behavioural observations of reproduction and mate choice in wild fossorial rodents are extremely limited and consequently indirect methods are typically used to infer mating strategies. We use a combination of morphological, reproductive, spatial, and genetic data to investigate the reproductive strategy of a solitary endemic species, the Cape dune mole-rat Bathyergus suillus. These data provide the first account on the population dynamics of this species. Marked sexual dimorphism was apparent with males being both significantly larger and heavier than females. Of all females sampled 36% had previously reproduced and 12% were pregnant at the time of capture. Post-partum sex ratio was found to be significantly skewed in favour of females. The paternity of fifteen litters (n = 37 was calculated, with sires assigned to progeny using both categorical and full probability methods, and including a distance function. The maximum distance between progeny and a putative sire was determined as 2149 m with males moving between sub-populations. We suggest that above-ground movement should not be ignored in the consideration of mate acquisition behaviour of subterranean mammals. Estimated levels of multiple paternity were shown to be potentially as high as 26%, as determined using sibship and sire assignment methods. Such high levels of multiple paternity have not been found in other solitary mole-rat species. The data therefore suggest polyandry with no evidence as yet for polygyny.

  2. Relations between social status and the gonadotrophin-releasing hormone system in females of two cooperatively breeding species of African mole-rats, Cryptomys hottentotus hottentotus and Cryptomys hottentotus pretoriae: neuroanatomical and neuroendocrinological studies.

    Science.gov (United States)

    Du Toit, Lydia; Bennett, Nigel C; Katz, Arieh A; Kalló, Imre; Coen, Clive W

    2006-01-10

    In common (Cryptomys hottentotus hottentotus) and highveld (Cryptomys hottentotus pretoriae) mole-rats, reproduction is subject to two forms of regulation in addition to incest avoidance. These are the only social bathyergids known to restrict breeding to a particular season; furthermore, subordinate members of their colonies show suppressed reproduction throughout the year. Females from both species were assessed and compared for social and seasonal effects on the gonadotrophin-releasing hormone (GnRH) system. GnRH-immunoreactive (ir) structures were visualized immunohistochemically; GnRH content was determined by radioimmunoassay. In both species, GnRH-ir cell bodies and processes are loosely distributed along the septopreopticoinfundibular continuum, with dense fiber aggregations in the region of the organum vasculosum of the lamina terminalis and median eminence. The two species differ in the rostrocaudal distribution of their GnRH-ir cell bodies. In highveld mole-rats, most of these cells are in the septal/preoptic area; in common mole-rats, more than half of them are in the mediobasal hypothalamus. Compared with common mole-rats, highveld mole-rats have a greater total number of GnRH-ir cell bodies, higher GnRH content, and more intense GnRH immunoreactivity in the median eminence. Within highveld colonies, the nonreproductive females have larger GnRH-ir cell bodies, more intense GnRH immunoreactivity in the median eminence, and higher GnRH content than the reproductive females; these findings suggest inhibited release of GnRH in the nonreproductive, subordinate females. In contrast, in common mole-rat females, neither status nor season appears to affect the investigated parameters of the GnRH system; this suggests a predominantly behavioral basis to their suppressed reproduction.

  3. Naked Black Hole Firewalls.

    Science.gov (United States)

    Chen, Pisin; Ong, Yen Chin; Page, Don N; Sasaki, Misao; Yeom, Dong-Han

    2016-04-22

    In the firewall proposal, it is assumed that the firewall lies near the event horizon and should not be observable except by infalling observers, who are presumably terminated at the firewall. However, if the firewall is located near where the horizon would have been, based on the spacetime evolution up to that time, later quantum fluctuations of the Hawking emission rate can cause the "teleological" event horizon to have migrated to the inside of the firewall location, rendering the firewall naked. In principle, the firewall can be arbitrarily far outside the horizon. This casts doubt about the notion that firewalls are the "most conservative" solution to the information loss paradox.

  4. Gravitational collapse and naked singularities

    Indian Academy of Sciences (India)

    Tomohiro Harada

    2004-10-01

    Gravitational collapse is one of the most striking phenomena in gravitational physics. The cosmic censorship conjecture has provided strong motivation for research in this field. In the absence of a general proof for censorship, many examples have been proposed, in which naked singularity is the outcome of gravitational collapse. Recent developments have revealed that there are examples of naked singularity formation in the collapse of physically reasonable matter fields, although the stability of these examples is still uncertain. We propose the concept of `effective naked singularities', which will be quite helpful because general relativity has limitation in its application at the high-energy end. The appearance of naked singularities is not detestable but can open a window for the new physics of strongly curved space-times.

  5. Dilatonic effects near naked singularities

    CERN Document Server

    Morris, J R

    2011-01-01

    Static spherically symmetric solutions of 4d Brans-Dicke theory include a set of naked singularity solutions. Dilatonic effects near the naked singularities result in either a shielding or an antishielding effect from intruding massive test particles. One result is that for a portion of the solution parameter space, no communication between the singularity and a distant observer is possible via massive particle exchanges. Kaluza-Klein gravity is considered as a special case.

  6. Can we see naked singularities?

    OpenAIRE

    Deshingkar, Shrirang S.

    2007-01-01

    We study singularities which can form in a spherically symmetric gravitational collapse of a general matter field obeying weak energy condition. We show that no energy can reach an outside observer from a null naked singularity. That means they will not be a serious threat to the Cosmic Censorship Conjecture (CCC). For the timelike naked singularities, where only the central shell gets singular, the redshift is always finite and they can in principle, carry energy to a faraway observer. Hence...

  7. Circadian genes in a blind subterranean mammal II: conservation and uniqueness of the three Period homologs in the blind subterranean mole rat, Spalax ehrenbergi superspecies.

    Science.gov (United States)

    Avivi, Aaron; Oster, Henrik; Joel, Alma; Beiles, Avigdor; Albrecht, Urs; Nevo, Eviatar

    2002-09-03

    We demonstrated that a subterranean, visually blind mammal has a functional set of three Per genes that are important components of the circadian clockwork in mammals. The mole rat superspecies Spalax ehrenbergi is a blind subterranean animal that lives its entire life underground in darkness. It has degenerated eyes, but the retina and highly hypertrophic harderian gland are involved in photoperiodic perception. All three Per genes oscillate with a periodicity of 24 h in the suprachiasmatic nuclei, eye, and harderian gland and are expressed in peripheral organs. This oscillation is maintained under constant conditions. The light inducibility of sPer1 and sPer2, which are similar in structure to those of other mammals, indicates the role of these genes in clock resetting. However, sPer3 is unique in mammals and has two truncated isoforms, and its expressional analysis leaves its function unresolved. Per's expression analysis in the harderian gland suggests an important participation of this organ in the stabilization and resetting mechanism of the central pacemaker in the suprachiasmatic nuclei and in unique adaptation to life underground.

  8. Magnetic topology of a naked sunspot: Is it really naked?

    CERN Document Server

    Dalda, A Sainz; Tarbell, T D; 10.1088/2041-8205/746/1/L13

    2012-01-01

    The high spatial, temporal and spectral resolution achieved by Hinode instruments give much better understanding of the behavior of some elusive solar features, such as pores and naked sunspots. Their fast evolution and, in some cases, their small sizes have made their study difficult. The moving magnetic features, despite being more dynamic structures, have been studied during the last 40 years. They have been always associated with sunspots, especially with the penumbra. However, a recent observation of a naked sunspot (one with no penumbra) has shown MMF activity. The authors of this reported observation expressed their reservations about the explanation given to the bipolar MMF activity as an extension of the penumbral filaments into the moat. How can this type of MMFs exist when a penumbra does not? In this paper, we study the full magnetic and (horizontal) velocity topology of the same naked sunspot, showing how the existence of a magnetic field topology similar to that observed in sunspots can explain ...

  9. Naked singularities as particle accelerators

    CERN Document Server

    Patil, Mandar; 10.1103/PhysRevD.82.104049

    2010-01-01

    We investigate here the particle acceleration by naked singularities to arbitrarily high center of mass energies. Recently it has been suggested that black holes could be used as particle accelerators to probe the Planck scale physics. We show that the naked singularities serve the same purpose and probably would do better than their black hole counterparts. We focus on the scenario of a self-similar gravitational collapse starting from a regular initial data, leading to the formation of a globally naked singularity. It is seen that when particles moving along timelike geodesics interact and collide near the Cauchy horizon, the energy of collision in the center of mass frame will be arbitrarily high, thus offering a window to Planck scale physics.

  10. How Does Naked Singularity Look?

    OpenAIRE

    Nakao, Ken-ichi; Kobayashi, Naoki; Ishihara, Hideki

    2002-01-01

    There are non-radial null geodesics emanating from the shell focusing singularity formed at the symmetric center in a spherically symmetric dust collapse. In this article, assuming the self-similarity in the region filled with the dust fluid, we study these singular null geodesics in detail. We see the time evolution of the angular diameter of the central naked singularity and show that it might be bounded above by the value corresponding to the circular null geodesic in the Schwarzschild spa...

  11. Shadow of a naked singularity

    Science.gov (United States)

    Ortiz, Néstor; Sarbach, Olivier; Zannias, Thomas

    2015-08-01

    We analyze the redshift suffered by photons originating from an external source, traversing a collapsing dust cloud, and finally being received by an asymptotic observer. In addition, we study the shadow that the collapsing cloud casts on the sky of the asymptotic observer. We find that the resulting redshift and properties of the shadow depend crucially on whether the final outcome of the complete gravitational collapse is a black hole or a naked singularity. In the black hole case, the shadow is due to the high redshift acquired by the photons as they approach the event horizon, implying that their energy is gradually redshifted toward zero within a few crossing times associated with the event horizon radius. In contrast to this, a naked singularity not only absorbs photons originating from the source, but it also emits infinitely redshifted photons with and without angular momenta. This emission introduces an abrupt cutoff in the frequency shift of the photons detected in directions close to the radial one, and it is responsible for the shadow masking the source in the naked singularity case. Furthermore, even though the shadow forms and begins to grow immediately after the observer crosses the Cauchy horizon, it takes many more crossing times than in the black hole case for the source to be occulted from the observer's eyes. We discuss possible implications of our results for testing the weak cosmic censorship hypothesis. Even though at late times the image of the source perceived by the observer looks the same in both cases, the dynamical formation of the shadow and the redshift images has distinct features and time scales in the black hole versus the naked singularity case. For stellar collapse, these time scales seem to be too short to be resolved with existing technology. However, our results may be relevant for the collapse of seeds leading to supermassive black holes.

  12. Quantum dress for a naked singularity

    Science.gov (United States)

    Casals, Marc; Fabbri, Alessandro; Martínez, Cristián; Zanelli, Jorge

    2016-09-01

    We investigate semiclassical backreaction on a conical naked singularity space-time with a negative cosmological constant in (2 + 1)-dimensions. In particular, we calculate the renormalized quantum stress-energy tensor for a conformally coupled scalar field on such naked singularity space-time. We then obtain the backreacted metric via the semiclassical Einstein equations. We show that, in the regime where the semiclassical approximation can be trusted, backreaction dresses the naked singularity with an event horizon, thus enforcing (weak) cosmic censorship.

  13. Quantum dress for a naked singularity

    CERN Document Server

    Casals, Marc; Martínez, Cristián; Zanelli, Jorge

    2016-01-01

    We investigate semiclassical backreaction on a conical naked singularity space-time with a negative cosmological constant in (2+1)-dimensions. In particular, we calculate the renormalized quantum stress-energy tensor for a conformally coupled scalar field on such naked singularity space-time. We then obtain the backreacted metric via the semiclassical Einstein equations. We show that, in the regime where the semiclassical approximation can be trusted, backreaction dresses the naked singularity with an event horizon, thus enforcing cosmic censorship.

  14. Quantum dress for a naked singularity

    Directory of Open Access Journals (Sweden)

    Marc Casals

    2016-09-01

    Full Text Available We investigate semiclassical backreaction on a conical naked singularity space–time with a negative cosmological constant in (2+1-dimensions. In particular, we calculate the renormalized quantum stress–energy tensor for a conformally coupled scalar field on such naked singularity space–time. We then obtain the backreacted metric via the semiclassical Einstein equations. We show that, in the regime where the semiclassical approximation can be trusted, backreaction dresses the naked singularity with an event horizon, thus enforcing (weak cosmic censorship.

  15. Naked Singularities as Particle Accelerators II

    CERN Document Server

    Patil, Mandar; Malafarina, Daniele

    2011-01-01

    We generalize here our earlier results on particle acceleration by naked singularities. We showed recently[1] that the naked singularities that form due to gravitational collapse of massive stars provide a suitable environment where particles could get accelerated and collide at arbitrarily high center of mass energies. However, we focussed there only on the spherically symmetric gravitational collapse models, which were also assumed to be self-similar. In this paper, we broaden and generalize the result to all gravitational collapse models leading to the formation of a naked singularity as final state of collapse, evolving from a regular initial data, without making any prior restrictive assumptions about the spacetime symmetries such as above. We show that when the particles interact and collide near the Cauchy horizon, the energy of collision in the center of mass frame will be arbitrarily high, thus offering a window to the Planck scale physics. We also consider the issue of various possible physical mech...

  16. Dark matter annihilation near a naked singularity

    CERN Document Server

    Patil, Mandar

    2011-01-01

    We investigate here the dark matter annihilation near a Kerr naked singularity. We show that when dark matter particles collide and annihilate in vicinity of the singularity, the escape fraction to infinity of particles produced is much larger, at least 10^2 - 10^3 times the corresponding black hole values. As high energy collisions are generically possible near a naked singularity, this provides an excellent environment for efficient conversion of dark matter into ordinary standard model particles. If the center of galaxy harbored such a naked singularity, it follows that the observed emergent flux of particles with energy comparable to mass of the dark matter particles is much larger compared to the blackhole case, thus providing an intriguing observational test on the nature of the galactic center

  17. Tidal Forces in Naked Singularity Backgrounds

    CERN Document Server

    Goel, Akash; Roy, Pratim; Sarkar, Tapobrata

    2015-01-01

    The end stage of a gravitational collapse process can generically result in a black hole or a naked singularity. Here we undertake a comparative analysis of the nature of tidal forces in these backgrounds. The effect of such forces is generically exemplified by the Roche limit, which predicts the distance within which a celestial object disintegrates due to the tidal effects of a second more massive object. In this paper, using Fermi normal coordinates, we numerically compute the Roche limit for a class of non-rotating naked singularity backgrounds, and compare them with known results for Schwarzschild black holes. Our analysis indicates that there might be substantially large deviations in the magnitudes of tidal forces in naked singularity backgrounds, compared to the black hole cases. If observationally established, these can prove to be an effective indicator of the nature of the singularity at a galactic centre.

  18. Non-image Forming Light Detection by Melanopsin, Rhodopsin, and Long-Middlewave (L/W) Cone Opsin in the Subterranean Blind Mole Rat, Spalax Ehrenbergi: Immunohistochemical Characterization, Distribution, and Connectivity.

    Science.gov (United States)

    Esquiva, Gema; Avivi, Aaron; Hannibal, Jens

    2016-01-01

    The blind mole rat, Spalax ehrenbergi, can, despite severely degenerated eyes covered by fur, entrain to the daily light/dark cycle and adapt to seasonal changes due to an intact circadian timing system. The present study demonstrates that the Spalax retina contains a photoreceptor layer, an outer nuclear layer (ONL), an outer plexiform layer (OPL), an inner nuclear layer (INL), an inner plexiform layer (IPL), and a ganglion cell layer (GCL). By immunohistochemistry, the number of melanopsin (mRGCs) and non-melanopsin bearing retinal ganglion cells was analyzed in detail. Using the ganglion cell marker RNA-binding protein with multiple splicing (RBPMS) it was shown that the Spalax eye contains 890 ± 62 RGCs. Of these, 87% (752 ± 40) contain melanopsin (cell density 788 melanopsin RGCs/mm(2)). The remaining RGCs were shown to co-store Brn3a and calretinin. The melanopsin cells were located mainly in the GCL with projections forming two dendritic plexuses located in the inner part of the IPL and in the OPL. Few melanopsin dendrites were also found in the ONL. The Spalax retina is rich in rhodopsin and long/middle wave (L/M) cone opsin bearing photoreceptor cells. By using Ctbp2 as a marker for ribbon synapses, both rods and L/M cone ribbons containing pedicles in the OPL were found in close apposition with melanopsin dendrites in the outer plexus suggesting direct synaptic contact. A subset of cone bipolar cells and all photoreceptor cells contain recoverin while a subset of bipolar and amacrine cells contain calretinin. The calretinin expressing amacrine cells seemed to form synaptic contacts with rhodopsin containing photoreceptor cells in the OPL and contacts with melanopsin cell bodies and dendrites in the IPL. The study demonstrates the complex retinal circuitry used by the Spalax to detect light, and provides evidence for both melanopsin and non-melanopsin projecting pathways to the brain.

  19. Kerr Naked Singularities as Particle Accelerators

    CERN Document Server

    Patil, Mandar

    2011-01-01

    We investigate here the particle acceleration by Kerr naked singularities. We consider a collision between particles dropped in from infinity at rest, which follow geodesic motion in the equatorial plane, with angular momentum of one of the particles in an appropriate finite range of values. The absence of an event horizon and the repulsive nature of angular momentum makes it possible for the initially infalling particle to turn back as an outgoing particle and then collide with another infalling particle. When these particles collide at a location close to what would have been the event horizon in the extremal Kerr blackhole case, the center of mass energy of collision turns out to be arbitrarily large depending on how close is the Kerr naked singularity to extremality. We briefly discuss the possible astrophysical consequences of this process and suggest that the fast rotating Kerr configurations could provide a good cosmic laboratory to probe ultra-high-energy physics.

  20. The shadow of a naked singularity

    CERN Document Server

    Ortiz, Néstor; Zannias, Thomas

    2015-01-01

    We analyze the redshift suffered by photons originating from an external source, traversing a collapsing dust cloud and finally received by an asymptotic observer. In addition, we study the shadow that the collapsing cloud casts on the sky of the asymptotic observer. We find that the resulting redshift and properties of the shadow depend crucially on whether the final outcome of the complete gravitational collapse is a black hole or a naked singularity. In the black hole case, the shadow is due to the high redshift acquired by the photons as they approach the event horizon, implying that their energy is gradually redshifted towards zero within a few crossing times associated with the event horizon radius. In contrast to this, a naked singularity not only absorbs photons originating from the source, but it also emits infinitely redshifted photons with and without angular momenta. This emission introduces an abrupt cut-off in the frequency shift of the photons detected in directions close to the radial one, and...

  1. Naked-eye astronomy in mass tourism

    Directory of Open Access Journals (Sweden)

    Tadić Milutin

    2016-01-01

    Full Text Available This paper considers the possibility of developing astro-tourism in small countries such as Serbia and other Balkan countries, with "discreet" astro-tourism reduced to naked-eye astronomy as part of mass tourism rather than elite tourism that is realized through specialized programs with spacecraft and space stations, spectacular planetariums and observatories with giant telescopes. The specific relationship between the virtual and the real world is highlighted in this paper especially when considering younger generations. Furthermore, the differences in the way stars are perceived by astronomers and "ordinary" tourists or non-astronomers is also emphasized. Tourists are not enchanted by celestial bodies themselves, but by a complete scene, which consists of integrated skyscapes and landscapes, as a complex stage where in various combinations and forms these celestial bodies are observed, and of their own experience. These complex sky-earth landscapes, astro-landscapes, present astro-tourism resources of the small countries like Serbia, provided that a tourist can "read" their astronomical text. In this regard, Belgrade was used as the example for drafting a "package" of astronomical information that can be easily prepared and adapted according to the needs of a particular group, so that every tourist may know what can be seen, where and when. With the help of a rotating star map (planisphere, visitors may observe the sky with the naked eye.

  2. Naked Singularities in Spherically Symmetric, Self-Similar Spacetimes

    CERN Document Server

    Wagh, S M; Wagh, Sanjay M.; Govinder, Keshlan S.

    2001-01-01

    We show that all known naked singularities in spherically symmetric self-similar spacetimes arise as a result of singular initial matter distribution. This is a result of the peculiarity of the coordinate transformation that takes these spacetimes into a separable form. Therefore, these examples of naked singularities are of no apparent consequence to astrophysical observations or theories.

  3. Repeated functional convergent effects of NaV1.7 on acid insensitivity in hibernating mammals.

    Science.gov (United States)

    Liu, Zhen; Wang, Wei; Zhang, Tong-Zuo; Li, Gong-Hua; He, Kai; Huang, Jing-Fei; Jiang, Xue-Long; Murphy, Robert W; Shi, Peng

    2014-02-07

    Hibernating mammals need to be insensitive to acid in order to cope with conditions of high CO2; however, the molecular basis of acid tolerance remains largely unknown. The African naked mole-rat (Heterocephalus glaber) and hibernating mammals share similar environments and physiological features. In the naked mole-rat, acid insensitivity has been shown to be conferred by the functional motif of the sodium ion channel NaV1.7. There is now an opportunity to evaluate acid insensitivity in other taxa. In this study, we tested for functional convergence of NaV1.7 in 71 species of mammals, including 22 species that hibernate. Our analyses revealed a functional convergence of amino acid sequences, which occurred at least six times independently in mammals that hibernate. Evolutionary analyses determined that the convergence results from both parallel and divergent evolution of residues in the functional motif. Our findings not only identify the functional molecules responsible for acid insensitivity in hibernating mammals, but also open new avenues to elucidate the molecular underpinnings of acid insensitivity in mammals.

  4. Ecology of mole rats Tachyoryctes splendens and its impact on farmlands at Angacha, central Ethiopia%长毛速掘鼠的生态学以及对埃塞俄比亚中部Angacha地区农田的影响

    Institute of Scientific and Technical Information of China (English)

    Abebe KOKISO; Afework BEKELE

    2008-01-01

    Studies on the distribution, abundance and impact of mole rat Tachyoryctes splendens in farmlands at Angacha, Ethiopia, were carried out from August 2005 to April 2006 covering both wet and dry seasons. Direct total count was carried out from fresh mounds to estimate the abundance and distribution of mole rats. Stomach content analysis was carried out to determine the food contents of mole rats at four sites representing "Dega" (cool) and "Woina-dega" (warm) temperate climatic zones. Total count by digging and using local trap catches depicted that "Dega" climatic zone sites, Lemi-suticho and Serera-bokata, harbored more mole rat population, 12 and 15 individuals /ha, respectively than "Woina-dega"climatic zone sites, Angacha-01 (7/ha) and Kerekicho (10/ha). 31 individuals consisting of 22 adults and nine juveniles, were captured. Male to female sex ratito nearby crop fields. Drainage, fertility of soil and availability of food resources were the main factors that reduced the length of burrow systems (11.3-18.6m). There was no significant difference in the length and depth of foraging tunnel, bolthole, and nest between males and females. Loss on enset plantation Ensete ventricosum is about 12% for the whole district. Such loss can greatly affect the economy of people where there is a large family size. Mole rats are the major rodent pests on enset and potatoes though their effect on other crops is minor. It is crucial to devise a proper control method to minimize the impact on economically important food enset.%于2005年8月-2006年4月研究了埃塞俄比亚Angacha地区农田中的长毛速掘鼠的分布、多度以及影响.通过直接计数地面的新隆起来估计速掘鼠的分布和多度,通过分析胃容物确定了四个地区(分别代表了"凉爽"和"温暖"的温带气候区)速掘鼠的食物成分.通过挖洞和鼠夹捕捉发现,两个凉爽区域内速掘鼠的数量分别为12只/ha和15只/ha,而温暖区域内的数量分别为7

  5. A naked-eye colorimetric "PCR developer"

    Science.gov (United States)

    Valentini, Paola; Pompa, Pier Paolo

    2016-04-01

    Despite several advances in molecular biology and diagnostics, Polymerase Chain Reaction (PCR) is currently the gold standard for nucleic acids amplification and detection, due to its versatility, low-cost and universality, with estimated market of several billion dollars/year. Nevertheless, PCR still relies on the laborious, time-consuming, and multi-step gel electrophoresis-based detection, which includes gel casting, electrophoretic run, gel staining, and gel visualization. In this work, we propose a "PCR developer", namely a universal one-step, one-tube method, based on controlled aggregation of gold nanoparticles (AuNPs), to detect PCR products by naked eye in few minutes, with no need for any instrumentation. We demonstrated the specificity and sensitivity of the PCR developer on different model targets, suitable for a qualitative detection in real-world diagnostics (i.e., gene rearrangements, genetically modified organisms, and pathogens). The PCR developer proved to be highly specific and ultra-sensitive, discriminating down to few copies of HIV viral DNA, diluted in an excess of interfering human genomic DNA, which is a clinically relevant viral load. Hence, it could be a valuable tool for both academic research and clinical applications.

  6. Antiviral activity of lactoferrin towards naked viruses.

    Science.gov (United States)

    Seganti, Lucilla; Di Biase, Assunta Maria; Marchetti, Magda; Pietrantoni, Agostina; Tinari, Antonella; Superti, Fabiana

    2004-06-01

    It is well known that lactoferrin (Lf) is a potent inhibitor towards several enveloped and naked viruses, such as rotavirus, enterovirus and adenovirus. Lf is resistant to tryptic digestion and breast-fed infants excrete high levels of faecal Lf, so that its effect on viruses replicating in the gastrointestinal tract is of great interest. In this report, we analysed the mechanism of the antiviral action of this protein in three viral models which, despite representing different genoma and replication strategies, share the ability to infect the gut. Concerning the mechanism of action against rotavirus, Lf from bovine milk (BLf) possesses a dual role, preventing virus attachment to intestinal cells by binding to viral particles, and inhibiting a post adsorption step. The BLf effect towards poliovirus is due to the interference with an early infection step but, when the BLf molecule is saturated with Zn+2 ions, it is also capable of inhibiting viral replication after the viral adsorption phase. The anti-adenovirus action of BLf takes place on virus attachment to cell membranes through competition for common glycosaminoglycan receptors and a specific interaction with viral structural polypeptides. Taken together, these findings provide further evidence that Lf is an excellent candidate in the search of natural agents against viral enteric diseases, as it mainly acts by hindering adsorption and internalisation into cells through specific binding to cell receptors and/or viral particles.

  7. Dynamics And Thermodynamics Of Blackholes And Naked Singularities II

    CERN Document Server

    Fatibene, Lorenzo; GiambÒ, Roberto; Magli, Giulio

    2012-01-01

    Proceedings of the second edition of the international Workshop "Dynamics and Thermodynamics of Blackholes and Naked Singularities" (Department of Mathematics of the Politecnico of Milano from May 10-12, 2007.

  8. Dynamics and Thermodynamics of Blackholes and Naked Singularities

    OpenAIRE

    Fatibene, Lorenzo; Francaviglia, Mauro; Giambo', Roberto; Magli, Giulio

    2005-01-01

    Proceedings of the international Workshop on ``Dynamics and Thermodynamics of Blackholes and Naked Singularities``, that took place at the Department of Mathematics of the Politecnico of Milano from 13 to 15 May 2004.

  9. How to count nanoparticles with the naked eye?

    Science.gov (United States)

    Sinelnik, Artem D.; Samusev, Kirill B.

    2017-09-01

    In this work we experimentally investigated the patterns of optical Laue diffraction from photonic structures. The obtained experimental results and their interpretation allowed us to propose a very simple method of nanoparticles precise counting with the naked eye.

  10. Productive performance of naked neck, frizzle and dwarf laying hens ...

    African Journals Online (AJOL)

    Unknown

    various natural climates and two nutritional treatments ... improved egg production or feed efficiency, although the naked neck (Na) ... pullets were moved to a tiered system of individual cages in a laying house with ..... nutrients for production.

  11. Quantum naked singularities in 2d dilaton gravity

    CERN Document Server

    Vaz, C; Vaz, Cenalo; Witten, Louis

    1996-01-01

    Roughly speaking, naked singularities are singularities that may be seen by timelike observers. The Cosmic Censorship conjecture forbids their existence by stating that a reasonable system of energy will not, under reasonable conditions, collapse into a naked singularity. There are however many counter-examples to this conjecture in the literature. We propose a defense of the conjecture through the quantum theory. We will show that the Hawking effect, when consistently applied to naked singularities in two dimensional models of dilaton gravity with matter and a cosmological constant, prevents their formation by causing them to explode or catastrophically emit radiation, as opposed to black holes which radiate slowly. If this phenomenon is reproduced in the four dimensional world, the explosion of naked singularities should have observable consequences.

  12. Naked singularities in Tolman-Bondi-de Sitter collapse

    CERN Document Server

    Gonçalves, S

    2001-01-01

    We study the formation of central naked singularities in spherical dust collapse with a cosmological constant. We find that the central curvature singularity is locally naked, Tipler strong, and generic, in the sense that it forms from a non-zero-measure set of regular initial data. We also find that the Weyl and Ricci curvature scalars diverge at the singularity, with the former dominating over the latter, thereby signaling the non-local origin of the singularity.

  13. Mass Quadrupole as a Source of Naked Singularities

    CERN Document Server

    Quevedo, Hernando

    2010-01-01

    We investigate the gravitational field of a static mass with quadrupole moment in empty space. It is shown that in general this configuration is characterized by the presence of curvature singularities without a surrounding event horizon. These naked singularities generate an effective field of repulsive gravity which, in turn, drastically changes the behavior of test particles. As a possible consequence, the accretion disk around a naked singularity presents a particular discontinuous structure.

  14. A Nearly Naked Supermassive Black Hole

    Science.gov (United States)

    Condon, J. J.; Darling, Jeremy; Kovalev, Y. Y.; Petrov, L.

    2017-01-01

    During a systematic search for supermassive black holes (SMBHs) not in galactic nuclei, we identified the compact, symmetric radio source B3 1715+425 with an emission-line galaxy offset ≈ 8.5 {kpc} from the nucleus of the brightest cluster galaxy (BCG) in the redshift z = 0.1754 cluster ZwCl 8193. B3 1715+425 is too bright (brightness temperature {T}{{b}}∼ 3× {10}10 {{K}} at observing frequency ν =7.6 {GHz}) and too luminous (1.4 GHz luminosity {L}1.4{GHz}∼ {10}25 {{W}} {{Hz}}-1) to be powered by anything but an SMBH, but its host galaxy is much smaller (∼ 0.9 {kpc}× 0.6 {kpc} full width between half-maximum points) and optically fainter (R-band absolute magnitude {M}{{r}}≈ -18.2) than any other radio galaxy. Its high radial velocity {v}{{r}}≈ 1860 {km} {{{s}}}-1 relative to the BCG, continuous ionized wake extending back to the BCG nucleus, and surrounding debris indicate that the radio galaxy was tidally shredded passing through the BCG core, leaving a nearly naked SMBH fleeing from the BCG with space velocity v≳ 2000 {km} {{{s}}}-1. The radio galaxy has mass M≲ 6× {10}9 {M}ȯ and infrared luminosity {L}{IR}∼ 3× {10}11 {L}ȯ close to its dust Eddington limit, so it is vulnerable to further mass loss from radiative feedback.

  15. Perfect fluid tori orbiting Kehagias-Sfetsos naked singularities

    CERN Document Server

    Stuchlík, Zdeněk; Schee, Jan; Kučáková, Hana

    2014-01-01

    We construct perfect fluid tori in the field of the Kehagias-Sfetsos (K-S) naked singularity representing spherically symmetric vacuum solution of the modified Ho\\v{r}ava quantum gravity that is characterized by a dimensionless parameter $\\omega M^2$, combining the gravitational mass parameter $M$ of the spacetime with the Ho\\v{r}ava parameter $\\omega$ reflecting the role of the quantum corrections. In dependence on the value of $\\omega M^2$, the K-S naked singularities demonstrate a variety of qualitatively different behavior of their circular geodesics that is fully reflected in the properties of the toroidal structures. In all of the K-S naked singularity spacetimes the tori are located above an "antigravity" sphere where matter can stay in stable equilibrium position, that is relevant for the stability of the orbiting fluid toroidal accretion structures.

  16. Gravastars with Dark Energy Evolving to Naked Singularity

    CERN Document Server

    Chan, R; Senna, P; da Rocha, J F Villas

    2011-01-01

    We consider a gravastar model made of anisotropic dark energy with an infinitely thin spherical shell of a perfect fluid with the equation of state $p = (1-\\gamma)\\sigma$ with an external de Sitter-Schwarzschild region. It is found that in some cases the models represent the "bounded excursion" stable gravastars, where the thin shell is oscillating between two finite radii, while in other cases they collapse until the formation of black holes or naked singularities. An interesting result is that we can have black hole and stable gravastar formation even with an interior and a shell constituted of dark and repulsive dark energy, as also shown in previous work. Besides, in three cases we have a dynamical evolution to a black hole (for $\\Lambda=0$) or to a naked singularity (for $\\Lambda > 0$). This is the first time in the literature that a naked singularity emerges from a gravastar model.

  17. Breeding and feeding ecology of pigeon guillemots (Cepphus columba) at Naked Island, Alaska

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report covers the breeding and feeding ecology of the pigeon guillemot at Naked Island, Alaska, as well as surveys of the Naked Island complex. Methods...

  18. Spin precession in a black hole and naked singularity spacetimes

    Science.gov (United States)

    Chakraborty, Chandrachur; Kocherlakota, Prashant; Joshi, Pankaj S.

    2017-02-01

    We propose here a specific criterion to address the existence or otherwise of Kerr naked singularities, in terms of the precession of the spin of a test gyroscope due to the frame dragging by the central spinning body. We show that there is indeed an important characteristic difference in the behavior of gyro spin precession frequency in the limit of approach to these compact objects, and this can be used, in principle, to differentiate the naked singularity from a black hole. Specifically, if gyroscopes are fixed all along the polar axis up to the horizon of a Kerr black hole, the precession frequency becomes arbitrarily high, blowing up as the event horizon is approached. On the other hand, in the case of naked singularity, this frequency remains always finite and well behaved. Interestingly, this behavior is intimately related to and is governed by the geometry of the ergoregion in each of these cases, which we analyze here. One intriguing behavior that emerges is, in the Kerr naked singularity case, the Lense-Thirring precession frequency (ΩLT ) of the gyroscope due to frame-dragging effect decreases as (ΩLT∝r ) after reaching a maximum, in the limit of r =0 , as opposed to r-3 dependence in all other known astrophysical cases.

  19. Naked at Our Age: Talking out Loud about Senior Sex

    Science.gov (United States)

    Davis, Melanie

    2012-01-01

    "Naked at Our Age" is an excellent resource for sexually interested and/or active adults over the age of 60. The book combines the author's personal reflections, questions and stories shared by older adults, and advice from sex therapists, sexuality educators, the author, and health care providers. The breadth of topics makes the book useful to…

  20. Two-Laser Interference Visible to the Naked Eye

    Science.gov (United States)

    Kawalec, Tomasz; Bartoszek-Bober, Dobroslawa

    2012-01-01

    An experimental setup allowing the observation of two-laser interference by the naked eye is described. The key concept is the use of an electronic phase lock between two external cavity diode lasers. The experiment is suitable both for undergraduate and graduate students, mainly in atomic physics laboratories. It gives an opportunity for…

  1. Naked exclusion in the lab : The case of sequential contracting

    NARCIS (Netherlands)

    Boone, J.; Müller, W.; Suetens, S.

    2014-01-01

    In the context of the naked exclusion model of Rasmusen, Ramseyer and Wiley [1991] and Segal and Whinston [2000b], we examine whether sequential contracting is more conducive to exclusion in the lab, and whether it is cheaper for the incumbent than simultaneous contracting. We find that an incumbent

  2. Scalar Radiation in the Background of a Naked Singularity

    CERN Document Server

    Dey, Anshuman; Sarkar, Tapobrata

    2013-01-01

    We study scalar radiation spectra from a particle in circular orbit, in the background of the Janis-Newman-Winicour (JNW) naked singularity. The differences in the nature of the spectra, from what one obtains with a Schwarzschild black hole, is established. We also compute the angular distribution of the spectra.

  3. Perfect fluid tori orbiting Kehagias-Sfetsos naked singularities

    Science.gov (United States)

    Stuchlík, Z.; Pugliese, D.; Schee, J.; Kučáková, H.

    2015-09-01

    We construct perfect fluid tori in the field of the Kehagias-Sfetsos (K-S) naked singularities. These are spherically symmetric vacuum solutions of the modified Hořava quantum gravity, characterized by a dimensionless parameter ω M^2, combining the gravitational mass parameter M of the spacetime with the Hořava parameter ω reflecting the role of the quantum corrections. In dependence on the value of ω M^2, the K-S naked singularities demonstrate a variety of qualitatively different behavior of their circular geodesics that is fully reflected in the properties of the toroidal structures, demonstrating clear distinction to the properties of the torii in the Schwarzschild spacetimes. In all of the K-S naked singularity spacetimes the tori are located above an "antigravity" sphere where matter can stay in a stable equilibrium position, which is relevant for the stability of the orbiting fluid toroidal accretion structures. The signature of the K-S naked singularity is given by the properties of marginally stable tori orbiting with the uniform distribution of the specific angular momentum of the fluid, l= const. In the K-S naked singularity spacetimes with ω M^2 > 0.2811, doubled tori with the same l= const can exist; mass transfer between the outer torus and the inner one is possible under appropriate conditions, while only outflow to the outer space is allowed in complementary conditions. In the K-S spacetimes with ω M^2 < 0.2811, accretion from cusped perfect fluid tori is not possible due to the non-existence of unstable circular geodesics.

  4. Molecular evolution of the hyaluronan synthase 2 gene in mammals: implications for adaptations to the subterranean niche and cancer resistance.

    Science.gov (United States)

    Faulkes, Christopher G; Davies, Kalina T J; Rossiter, Stephen J; Bennett, Nigel C

    2015-05-01

    The naked mole-rat (NMR) Heterocephalus glaber is a unique and fascinating mammal exhibiting many unusual adaptations to a subterranean lifestyle. The recent discovery of their resistance to cancer and exceptional longevity has opened up new and important avenues of research. Part of this resistance to cancer has been attributed to the fact that NMRs produce a modified form of hyaluronan--a key constituent of the extracellular matrix--that is thought to confer increased elasticity of the skin as an adaptation for living in narrow tunnels. This so-called high molecular mass hyaluronan (HMM-HA) stems from two apparently unique substitutions in the hyaluronan synthase 2 enzyme (HAS2). To test whether other subterranean mammals with similar selection pressures also show molecular adaptation in their HAS2 gene, we sequenced the HAS2 gene for 11 subterranean mammals and closely related species, and combined these with data from 57 other mammals. Comparative screening revealed that one of the two putatively important HAS2 substitutions in the NMR predicted to have a significant effect on hyaluronan synthase function was uniquely shared by all African mole-rats. Interestingly, we also identified multiple other amino acid substitutions in key domains of the HAS2 molecule, although the biological consequences of these for hyaluronan synthesis remain to be determined. Despite these results, we found evidence of strong purifying selection acting on the HAS2 gene across all mammals, and the NMR remains unique in its particular HAS2 sequence. Our results indicate that more work is needed to determine whether the apparent cancer resistance seen in NMR is shared by other members of the African mole-rat clade. © 2015 The Author(s) Published by the Royal Society. All rights reserved.

  5. Naked bodies, naked genomes: the special (but not exceptional) nature of genomic information.

    Science.gov (United States)

    Sulmasy, Daniel P

    2015-05-01

    Genetic exceptionalism, the view that genomic information is different from other types of sensitive information and deserves exceptional types of protections, has been roundly criticized. However, the public still expresses special fears about the access others might have to their genomic information. In this article, it is argued that there may be a basis for the public perception that genomic information is special, even if it cannot be said that policies could or should be enacted to protect the privacy and confidentiality of genomic information that would be exceptional relative to the protections one would enact to protect other types of sensitive information. The special nature of genomic information lies in understanding that it is neither personal property nor mere information. A genome is, at one and the same time, a physical aspect of a person and information about that person. Genomic data are embodied information that partially constitutes as well as describes individuals and that connects them in physical ways to their ancestors and their relatives. All forms of privacy need to be protected, but some intimate aspects of our lives command special respect. To see a genome is more analogous to seeing a naked body than to seeing a social security number. This metaphor suggests that clinicians and investigators ought to respect the special concerns of patients regarding genomic information while not claiming that there are any exceptional measures one could take to protect genomic privacy. Suggestions are given for how this view might affect patient interactions, consent discussions, public policy, and public trust in genomic research and clinical genetics.

  6. Naked singularity formation in Brans-Dicke theory

    Energy Technology Data Exchange (ETDEWEB)

    Ziaie, Amir Hadi; Atazadeh, Khedmat [Department of Physics, Shahid Beheshti University, Evin, Tehran 19839 (Iran, Islamic Republic of); Tavakoli, Yaser, E-mail: am.ziaie@mail.sbu.ac.i, E-mail: k-atazadeh@sbu.ac.i, E-mail: tavakoli@ubi.p [Departamento de Fisica, Universidade da Beira Interior, Rua Marques d' Avila e Bolama, 6200 Covilha (Portugal)

    2010-04-07

    Gravitational collapse of the Brans-Dicke scalar field with non-zero potential in the presence of matter fluid obeying the barotropic equation of state, p = wrho, is studied. Utilizing the concept of the expansion parameter, it is seen that the cosmic censorship conjecture may be violated for w=-1/3 and w=-2/3 which correspond to the cosmic string and domain wall, respectively. We have shown that physically, it is the rate of collapse that governs the formation of a black hole or a naked singularity as the final fate of dynamical evolution and only for these two cases can the singularity be naked as the collapse end state. Also the weak energy condition is satisfied by the collapsing configuration.

  7. Hybrid nanomaterials: anchoring magnetic molecules on naked gold nanocrystals.

    Science.gov (United States)

    Holmberg, Rebecca J; Hutchings, Amy-Jayne; Habib, Fatemah; Korobkov, Ilia; Scaiano, Juan C; Murugesu, Muralee

    2013-12-16

    The pairing of molecular magnets and nanomaterials couples top-down and bottom-up approaches to nanotechnology; facilitating a unique methodology to the controlled study of interfacial magnetic properties. Attaching Single-Molecule Magnets (SMMs) to "naked" gold nanoparticles is a novel method of exploring various avenues of magnetic nanotechnology, such as drug delivery, information storage, catalysis, and assembly of magnetic-nanostructural motifs. Herein we report the successful capping of laser ablation synthesized "naked" gold nanoparticles with a dinuclear dysprosium complex, while introducing new information regarding the changes in molecular magnetic properties upon surface attachment. We anticipate that this methodology in producing these magneto-plasmonic nanostructures not only provides answers to fundamental questions but also has the potential to provide new avenues to applications including information storage, multimodal imaging, biomedicine, and optoelectronics.

  8. Naked eye visibility of Sirius in broad daylight.

    Science.gov (United States)

    Können, Gunther P; Tinbergen, Jaap; Stammes, Piet

    2015-02-01

    Sirius was spotted with the naked eye at broad daylight by looking along the finder of a 1 m telescope on La Palma Observatory at a 2370 m height. Sun elevation was 73°; Sirius was nearly straight under the Sun at 37° elevation. The sky radiance, although not recorded directly, could be determined from the simultaneously obtained high-precision wavelength-dependent sky polarization data near Sirius. This was done by fitting the polarization data with the doubling-adding KNMI (DAK) radiative transfer model, which provided the values of the surface albedo and of the aerosol optical thickness required for determining the absolute sky radiance. Our analysis implies that Sirius, when positioned overhead, can be a daytime naked eye object from sea level even if its culmination occurs at solar noon. It also suggests that the second-brightest star (Canopus), if positioned overhead, could be perceptible even at solar noon.

  9. Quantum probes of timelike naked singularity with scalar hair

    OpenAIRE

    Svitek, O.; Tahamtan, T.

    2016-01-01

    We study the curvature singularity resolution via quantum fields on a fixed background based on Klein--Gordon and Dirac equations for a static spacetime with a scalar field producing a timelike naked singularity. We show that both Klein--Gordon and Dirac quantum fields see this singularity. Subsequently we check the results by applying the maximal acceleration existence in Covariant Loop Quantum Gravity described recently and obtain the resolution of singularity. In the process we study the g...

  10. NAKE'MUU: VILLAGE ON THE EDGE

    Energy Technology Data Exchange (ETDEWEB)

    B. VIERRA; ET AL

    2001-02-01

    Pursuant to federal requirements under the National Environmental Policy Act, in 1996 the Department of Energy (DOE) completed an environmental impact statement, record of decision, and mitigation action plan (MAP) for the Dual Axis Radiographic Hydrodynamic Test (DARHT) Facility. DARHT is a new explosives testing facility located at TA-15, Los Alamos National Laboratory (LANL). As part of the implementation of the MAP, the ESH-20 Cultural Resources Team at LANL is conducting a long-term monitoring program at the ancestral pueblo site of Nake'muu (LA 12655). DOE considers the monitoring program to be an appropriate and necessary mitigation for the potential operational impacts associated with the DARHT Facility. This chapter presents the preliminary findings of the monitoring program from 1997 through 2000. The Nake'muu site monitoring program was initiated by ESH-20 and the Mesa Verde Architectural Team, National Park Service (NPS) in 1997. Archaeologists from LANL and NPS have completed the detailed recording of all the walls at Nake'muu and the final site evaluation plan was finished in 1998 (Nordby et al. 1998). The plan describes the methods used for site monitoring, continued site revisits, and updates on wall condition by LANL archaeologists. The primary objective of the monitoring program is to identify and evaluate the long-term effects of the ambient environment and DARHT Facility operations on the architecture at Nake'muu. Is the dynamic-testing program affecting the site, and if so, to what degree? What are the short-term and long-term implications of facility operations?

  11. David Cronenberg, author of naked lunch: Intersemiotic translation as transcreation

    Directory of Open Access Journals (Sweden)

    Rosângela Fachel de Medeiros

    2016-05-01

    Full Text Available This paper presents and analyzes the intersemiotic translation process performed by filmmaker David Cronenberg of William Burroughs' novel Naked Lunch, and the intertextual bifurcations involved in this process. Investigating the similarities and differences between the creative worlds of both artists and how they reframe each other. For this, are used as the theoretical the idea of transcreation proposed by Haroldo de Campos, and the notion of the translator as a reader proposed by Jorge Luis Borges.

  12. Quantum probes of timelike naked singularity with scalar hair

    CERN Document Server

    Svítek, O

    2016-01-01

    We study the curvature singularity resolution via quantum fields on a fixed background based on Klein--Gordon and Dirac equations for a static spacetime with a scalar field producing a timelike naked singularity. We show that both Klein--Gordon and Dirac quantum fields see this singularity. Subsequently we check the results by applying the maximal acceleration existence in Covariant Loop Quantum Gravity described recently and obtain the resolution of singularity. In the process we study the geodesics in the spacetime.

  13. Naked singularities are not singular in distorted gravity

    Energy Technology Data Exchange (ETDEWEB)

    Garattini, Remo, E-mail: Remo.Garattini@unibg.it [Università degli Studi di Bergamo, Facoltà di Ingegneria, Viale Marconi 5, 24044 Dalmine (Bergamo) (Italy); I.N.F.N. – sezione di Milano, Milan (Italy); Majumder, Barun, E-mail: barunbasanta@iitgn.ac.in [Indian Institute of Technology Gandhinagar, Ahmedabad, Gujarat 382424 (India)

    2014-07-15

    We compute the Zero Point Energy (ZPE) induced by a naked singularity with the help of a reformulation of the Wheeler–DeWitt equation. A variational approach is used for the calculation with Gaussian Trial Wave Functionals. The one loop contribution of the graviton to the ZPE is extracted keeping under control the UltraViolet divergences by means of a distorted gravitational field. Two examples of distortion are taken under consideration: Gravity's Rainbow and Noncommutative Geometry. Surprisingly, we find that the ZPE is no more singular when we approach the singularity.

  14. The starry room naked eye astronomy in the intimate universe

    CERN Document Server

    Schaaf, Fred

    2002-01-01

    This inspiring and enriching book, a collection of essays that evokes the beauty and wonder of the night sky, explains to beginning skywatchers how to find and where to look for specific celestial objects. Author Fred Schaaf, once described as a naturalist in the tradition of Henry David Thoreau and John Burroughs, reveals an intense love of his subject and a keen knowledge of the optimum ways of viewing astronomical phenomena with the naked eye. Schaaf not only describes such special sights as an eyelash-thin moon, a shooting star, streaking comets, and a lunar eclipse, but he also explains w

  15. Negative Time Delay in Strongly Naked Singularity Lensing

    CERN Document Server

    DeAndrea, Justin P

    2014-01-01

    We model the supermassive galactic center of the Milky Way galaxy as a strongly naked singularity lens described by the Janis-Newman-Winicour metric. This metric has an ordinary mass and a massless scalar charge parameters. For very accurate results, we use Virbhadra-Ellis lens equation for computations. The galactic center serving as gravitational lens gives rise to 4 images: 2 images on the same side as the source and 2 images on the opposite side of the source from the optic axis. We compute positions and time delays of these images for many values of the angular source position. The time delays of primary images decrease with increase in the angular source position and is always negative. The time delays of the other 3 images are negative for small angular source position; however, they increase with an increase in angular source position. Such observations would support strongly naked singularity interpretation of the galactic center and, if ever observed, would disprove the cosmic censorship hypothesis ...

  16. A Nonsingular Brans Wormhole: An Analogue to Naked Black Holes

    CERN Document Server

    Bhattacharya, Amrita; Laserra, Ettore; Nandi, Kamal K

    2011-01-01

    In a recent paper, we showed the Jordan frame vacuum Brans Class I solution provided a wormhole analogue to Horowitz-Ross naked black hole in the wormhole range -3/2<{\\omega}<-4/3. Thereafter, the solution has been criticized by some authors that, because of the presence of singularity in that solution within this range, a wormhole interpretation of it is untenable. While the criticism is correct, we show here that (i) a singularity-free wormhole can actually be obtained from Class I solution by performing a kind of Wick rotation on it, resulting into what Brans listed as his independent Class II solution (ii) the Class II solution has all the necessary properties of a regular wormhole in a revised range -2<{\\omega}<-3/2 and finally, (iii) naked black holes, as described by Horowitz and Ross, are spacetimes where the tidal forces attain their maxima above the black hole horizon. We show that in the non-singular Class II spacetime this maxima is attained above the throat and thus can be treated as ...

  17. Charged null fluid collapse in anti-de Sitter spacetimes and naked singularities

    CERN Document Server

    Ghosh, S G

    2000-01-01

    We investigate the occurrence of naked singularities in the spherically symmetric, plane symmetric and cylindrically symmetric collapse of charged null fluid in an anti-de Sitter background. The naked singularities are found to be strong in Tipler's sense and thus violate the cosmic censorship conjecture, but not hoop conjecture.

  18. Formation and Evaporation of a Naked Singularity in 2D Gravity

    CERN Document Server

    Vaz, C; Vaz, Cenalo; Witten, Louis

    1994-01-01

    We describe a classical configuration of conformal matter forming a naked singularity and discuss its subsequent Hawking evaporation within the context of two dimensional dilaton gravity. The one loop analysis is credible for a large mass naked singularity and suggests the existence of a weak cosmological censorship that would cause it to explode into radiation upon forming. (Hardcopies of figures available on request)

  19. Study of Biometric Identification Method Based on Naked Footprint

    Directory of Open Access Journals (Sweden)

    Raji Rafiu King

    2013-10-01

    Full Text Available The scale of deployment of biometric identity-verification systems has recently seen an enormous increase owing to the need for more secure and reliable way of identifying people. Footprint identification which can be defined as the measurement of footprint features for recognizing the identity of a user has surfaced recently. This study is based on a biometric personal identification method using static footprint features viz. friction ridge / texture and foot shape / silhouette. To begin with, naked footprints of users are captured; images then undergo pre processing followed by the extraction of two features; shape using Gradient Vector Flow (GVF) snake model and minutiae extraction respectively. Matching is then effected based on these two features followed by a fusion of these two results for either a reject or accept decision. Our shape matching feature is based on cosine similarity while the texture one is based on miniature score matching. The results from our research establish that the naked footprint is a credible biometric feature as two barefoot impressions of an individual match perfectly while that of two different persons shows a great deal of dissimilarity. Normal 0 false false false IN X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Doi: 10.12777/ijse.5.2.29-35 How to cite this article: King

  20. Electromagnetic counterparts to gravitational waves from black hole mergers and naked singularities

    CERN Document Server

    Malafarina, Daniele

    2016-01-01

    We consider the question here whether the proposed electromagnetic counterpart of the gravitational wave signals in binary black hole coalescence may be due to the appearance of a `short lived' naked singularity during the merger. We point out that the change in topology that the spacetime undergoes during the merger can cause the appearance of a naked singularity. In case some matter, in the form of a small accretion disk, is present in the surroundings of the black hole system then the emitted luminosity during the merger would allow to distinguish the scenario where the naked singularity forms from the scenario where the horizon exists at all times. In fact the emitted luminosity spectrum would be much higher in the case where a naked singularity forms as opposed to the `pure' black hole case. We suggest that the presence of such a transient naked singularity will explain the high luminosity of an electromagnetic counterpart during the merger much more easily.

  1. Is it Really Naked? On Cosmic Censorship in String Theory

    Energy Technology Data Exchange (ETDEWEB)

    Frolov, A

    2004-09-30

    We investigate the possibility of cosmic censorship violation in string theory using a characteristic double-null code, which penetrates horizons and is capable of resolving the spacetime all the way to the singularity. We perform high-resolution numerical simulations of the evolution of negative mass initial scalar field profiles, which were argued to provide a counter example to cosmic censorship conjecture for AdS-asymptotic spacetimes in five-dimensional supergravity. In no instances formation of naked singularity is seen. Instead, numerical evidence indicates that black holes form in the collapse. Our results are consistent with earlier numerical studies, and explicitly show where the ''no black hole'' argument breaks.

  2. Agronomic performance of naked oat (Avena nuda L. and faba bean intercropping

    Directory of Open Access Journals (Sweden)

    Agnieszka Klimek-Kopyra

    2015-06-01

    Full Text Available The most common cereals for faba bean (Vicia faba L. used in intercrops is conventional oat (Avena sativa L. An alternative to oat may be naked oat (Avena nuda L., whose oil content and quality is double. Here, intercropping of naked oat with two different faba bean cultivars (determinate-high tannin and indeterminate-low tannin was compared with sole crops of each species in 2006-2008. The treatments were: sole naked oat at 500 kernels m², indeterminate sole faba bean at 50 seeds m², determinate sole faba bean at 70 seeds m², and an additive series of 25%, 50%, and 75% of faba bean seeding rate mixed with the naked oat seeding rate. Our results demonstrated that intercropping increased the Land Equivalent Ratio by +3% to +9% over sole cropping. Raising the faba bean seeding rate in a mixture from 25% to 75% reduced oat grain yield from 630 (determinate cultivar to 760 kg ha-1 (indeterminate cultivar but increased faba bean grain yield from 760 kg ha-1. Higher yield and leaf area index (LAI and photosynthetically active radiation (PAR values show that the indeterminate cultivar of faba bean is more suitable in mixture with naked oat. The high value of competition index (CR > 1 indicates domination and aggressiveness of faba bean towards naked oat. Regardless of cultivar type, mixture of faba bean with naked oat is less productive than pure sowing.

  3. The repulsive nature of naked singularities from the point of view of quantum mechanics

    Energy Technology Data Exchange (ETDEWEB)

    Batic, D.; Chin, D. [University of West Indies, Department of Mathematics, Kingston 6 (Jamaica); Nowakowski, M. [Universidad de los Andes, Departamento de Fisica, Cra. 1E No. 18A-10, Bogota (Colombia)

    2011-04-15

    We use the Dirac equation coupled to a background metric to examine what happens to quantum-mechanical observables like the probability density and the radial current in the vicinity of a naked singularity of the Reissner-Nordstroem type. We find that the wave function of the Dirac particle is regular in the point of the singularity. We show that the probability density is exactly zero at the singularity reflecting quantum-mechanically the repulsive nature of the naked singularity. Furthermore, the surface integral of the radial current over a sphere in the vicinity of the naked singularity turns out to be also zero. (orig.)

  4. TORTORA discovery of Naked-Eye Burst fast optical variability

    Science.gov (United States)

    Beskin, Grigory; Karpov, Sergey; Bondar, Sergey; Greco, Giuseppe; Guarnieri, Adriano; Bartolini, Corrado; Piccioni, Adalberto; Molinari, Emilio; Chincarini, Guido

    2008-10-01

    Features characterizing gamma-ray bursts in the different spectral bands may be a clue for the nature of their inner engine. Up to now, only several bursts have been observed in optical band during the gamma activity, and the only one-GRB080319B-was covered from rise till fall with high temporal resolution. Here we discuss these data, acquired with TORTORA fast wide-field monitoring optical camera, as well as results of its analysis. The camera observed the position of Naked-Eye Burst, GRB080318B, before, during and after the trigger. It detected the fast rise of optical emission, which reached the peak of V 5.3 at the eighteenth second, had a complex evolution till T+43s and monotonously faded then. The brightest part of the light curve contains two 15-20 s segments with different fluxes, each having two clearly-seen peaks of 5-8 s duration; all four peaks look quasi-periodic with separation of 9 s. There is no clear evidence of any sub-second variability. However, there are signs of quasi-periodic variability on 1s time scale at around the last peak (T+40 till T+50). The general properties of the optical light curve and its variability time scales look similar to the gamma one, but there is no clear correlation between them. This raises serious problems in interpretation of mechanisms generating such variability.

  5. Enhancement of naked FIX minigene expression by chloroquine in mice

    Institute of Scientific and Technical Information of China (English)

    Hong-yan CHEN; Huan-zhang ZHU; Bin LU; Xuan XU; Ji-hua YAO; Qi SHEN; Jing-lun XUE

    2004-01-01

    AIM: To study the effect of chloroquine on the expression of human clotting factor IX (hFIX) in mice. METHODS:Hydrodynamics-based naked DNA plasmid administration was performed by tail vein injection of 10 μg of pCMVhFIX and chloroquine (0, 100, 200, and 500 μmol/L) in 2.2 mL of Ringer' solution within 6-7 s, the level and stability of hFIX expression, liver damage and toxicity were then examined. RESULTS: The maximum expression of hFIX level was 4.4±-1.8 mg/L at 8 h after injection, 9.7±1.6 mg/L at 24 h only existed in 200 μmol/L chloroquinetreated animals, which is 3-4 fold higher than that of control (P<0.01). There is no significant difference observed among all the treated groups, 3 d later. Transaminase level and liver histological study showed the damage of liver was not related to chloroquine (P>0.05). CONCLUSION: Chloroquine can enhance and sustain exogenous gene expression in vivo without side effect under our experimental conditions.

  6. Daylight Observations of Venus with Naked Eye in the Goryeosa

    Directory of Open Access Journals (Sweden)

    Ki-Won Lee

    2017-03-01

    Full Text Available In this paper, we investigate the observations of Venus in daytime that are recorded in the Goryeosa (History of the Goryeo Dynasty, A.D. 918-1392. There are a total of 167 accounts of such observations in this historical book, spanning a period of 378 yr (from 1014 to 1392. These include six accounts where the days of the observation are not specified and two accounts where the phase angles are outside the calculation range of the equation used in our study. We analyze the number distribution of 164 accounts in 16 yr intervals covering the period from 1023 to 1391. We find that this distribution shows its minimum at around 1232, when the Goryeo dynasty moved the capital to the Ganghwa Island because of the Mongol invasion, and its maximum at around 1390, about the time when the dynasty fell. In addition, we calculate the azimuth, altitude, solar elongation, and apparent magnitude of Venus at sunset for 159 observations, excluding the eight accounts mentioned above, using the DE 406 ephemeris and modern astronomical algorithms. We find that the average elongation and magnitude of Venus on the days of those accounts were ~40° and -4.5, respectively, whereas the minimum magnitude was -3.8. The results of this study are useful for estimating the practical conditions for observing Venus in daylight with the naked eye and they also provide additional insight into the corresponding historical accounts contained in the Goryeosa.

  7. Stability of naked singularity arising in gravitational collapse of Type I matter fields

    Indian Academy of Sciences (India)

    Sanjay B Sarwe; R V Saraykar

    2005-07-01

    Considering gravitational collapse of Type I matter fields, we prove that, given an arbitrary 2-mass function (, ) and a 1-function ℎ(, ) (through the corresponding 1-metric function (, )), there exist infinitely many choices of energy distribution function () such that the `true’ initial data (, ℎ(, )) leads the collapse to the formation of naked singularity. We further prove that the occurrence of such a naked singularity is stable with respect to small changes in the initial data. We remark that though the initial data leading to both black hole (BH) and naked singularity (NS) form a `big' subset of the true initial data set, their occurrence is not generic. The terms `stability' and `genericity’ are appropriately defined following the theory of dynamical systems. The particular case of radial pressure () has been illustrated in details to get a clear picture of how naked singularity is formed and how, it is stable with respect to initial data.

  8. 莜麦及莜麦醋的营养成分分析%NutritionaI Ingredients AnaIysis of Naked Oats and Naked Oats Vinegar

    Institute of Scientific and Technical Information of China (English)

    杨丹丹; 于海峰

    2016-01-01

    The nutritional ingredients in naked oats vinegar and naked oats are analyzed in this paper. It is found that the ash content of naked oats vinegar and naked oats is 2.51%,1.32% respectively;the reducing sugar content is 2.53%,0.77% respectively;the total sugar content is 2.87%,1.04%respectively;the protein content is 5.02%,12.59% respectively;the total amino acids content is 2.08%,10.02% respectively;Ca is 142.34%,78.88% respectively;Mn is 4.94%,4.23%respectively;Zn is 2.85%,2.49% respectively;Fe is 15.20%,40.15% respectively;P is 404.02%, 210.30% respectively;Mg is 169.99%,77.74% respectively.The content of TMP in naked oats vinegar is 23.03 mg/L.Most of the nutritional ingredients in naked oats enter into the naked oats vinegar through the traditional solid-state brewing process.Naked oats vinegar is rich in nutrition and has good development prospect.%对莜麦醋及莜麦中的营养成分进行了分析测定。结果表明:莜麦醋与莜麦中分别含灰分2.51%,1.32%,还原糖2.53%,0.77%,总糖2.87%,1.04%,蛋白质5.02%,12.59%,氨基酸2.08%,10.02%,Ca 142.34%,78.88%,Mn 4.94%,4.23%,Zn 2.85%,2.49%,Fe 15.20%,40.15%, P 404.02%,210.30%,Mg 169.99%,77.74%,且莜麦醋中还含有川芎嗪23.03 mg/L。可见经传统的固态酿造过程,莜麦中大部分的营养成分都进入到了莜麦醋中。莜麦醋营养丰富,市场开发前景良好。

  9. Naked oats: metabolisable energy yield from a range of varieties in broilers, cockerels and turkeys.

    Science.gov (United States)

    MacLeod, M G; Valentine, J; Cowan, A; Wade, A; McNeill, L; Bernard, K

    2008-05-01

    1. Naked oats belong to the same species as 'common oats', Avena sativa, but have a non-lignified husk which readily becomes detached during harvesting. The absence of the indigestible husk can be predicted to give an increased metabolisable energy (ME) content for poultry. 2. Measurements of true metabolisable energy (TME(N)) were performed on 3-week-old broiler chicks (Cobb males), adult cockerels (ISA Brown, greater than one year old) and 7-week-old turkeys (BUT T8 males). The measurements were repeated in 2000, 2001 and 2002, with some measurements on a subset of varieties in 2004. 3. High-oil naked oat lines yielded 12% more energy (TME(N)) than wheat. Naked oats, excluding the experimental high-oil lines, yielded 8.5% more energy than simultaneously assayed wheat samples. 4. In samples from the 2004 harvest, conventional oats gave TME(N) values about 13% lower than those of wheat. 5. The addition of beta-glucanase produced an increase of about 4% in the apparent metabolisable energy (AME) of oats for broiler chickens. This effect was associated with a 70% decrease in the jejunal viscosity of broilers receiving a 500 g/kg naked-oat diet. 6. The oil content of naked oats was about 5 times greater than that of wheat, with the high-oil lines rising to more than 6 times greater. Naked oats had a lower starch content than wheat but not sufficiently lower to negate the energy benefits of the higher oil content. The crude protein (CP) contents of naked oats were similar to those of wheat, with the high-oil varieties tending to be higher in CP also.

  10. Oxidative Stress in Aging: Advances in Proteomic Approaches

    Directory of Open Access Journals (Sweden)

    Daniel Ortuño-Sahagún

    2014-01-01

    Full Text Available Aging is a gradual, complex process in which cells, tissues, organs, and the whole organism itself deteriorate in a progressive and irreversible manner that, in the majority of cases, implies pathological conditions that affect the individual’s Quality of Life (QOL. Although extensive research efforts in recent years have been made, the anticipation of aging and prophylactic or treatment strategies continue to experience major limitations. In this review, the focus is essentially on the compilation of the advances generated by cellular expression profile analysis through proteomics studies (two-dimensional [2D] electrophoresis and mass spectrometry [MS], which are currently used as an integral approach to study the aging process. Additionally, the relevance of the oxidative stress factors is discussed. Emphasis is placed on postmitotic tissues, such as neuronal, muscular, and red blood cells, which appear to be those most frequently studied with respect to aging. Additionally, models for the study of aging are discussed in a number of organisms, such as Caenorhabditis elegans, senescence-accelerated probe-8 mice (SAMP8, naked mole-rat (Heterocephalus glaber, and the beagle canine. Proteomic studies in specific tissues and organisms have revealed the extensive involvement of reactive oxygen species (ROS and oxidative stress in aging.

  11. Determinants of rodent longevity in the chaperone-protein degradation network.

    Science.gov (United States)

    Rodriguez, Karl A; Valentine, Joseph M; Kramer, David A; Gelfond, Jonathan A; Kristan, Deborah M; Nevo, Eviatar; Buffenstein, Rochelle

    2016-05-01

    Proteostasis is an integral component of healthy aging, ensuring maintenance of protein structural and functional integrity with concomitant impact upon health span and longevity. In most metazoans, increasing age is accompanied by a decline in protein quality control resulting in the accrual of damaged, self-aggregating cytotoxic proteins. A notable exception to this trend is observed in the longest-lived rodent, the naked mole-rat (NMR, Heterocephalus glaber) which maintains proteostasis and proteasome-mediated degradation and autophagy during aging. We hypothesized that high levels of the proteolytic degradation may enable better maintenance of proteostasis during aging contributing to enhanced species maximum lifespan potential (MLSP). We test this by examining proteasome activity, proteasome-related HSPs, the heat-shock factor 1 (HSF1) transcription factor, and several markers of autophagy in the liver and quadriceps muscles of eight rodent species with divergent MLSP. All subterranean-dwelling species had higher levels of proteasome activity and autophagy, possibly linked to having to dig in soils rich in heavy metals and where underground atmospheres have reduced oxygen availability. Even after correcting for phylogenetic relatedness, a significant (p rodents.

  12. Comparative genetics of longevity and cancer: insights from long-lived rodents

    Science.gov (United States)

    Gorbunova, Vera; Seluanov, Andrei; Zhang, Zhengdong; Gladyshev, Vadim N.; Vijg, Jan

    2015-01-01

    Mammals have evolved a dramatic diversity of aging rates. Within the single order of Rodentia maximum lifespans differ from four years in mice to 32 years in naked mole rats. Cancer rates also differ significantly, from cancer-prone mice to virtually cancer-proof naked and blind mole rats. Recent progress in rodent comparative biology, in combination with the emergence of whole genome sequence information, has opened opportunities for the discovery of genetic factors controlling longevity and cancer susceptibility. PMID:24981598

  13. PR72, a novel regulator of Wnt signaling required for Naked cuticle function

    NARCIS (Netherlands)

    Creyghton, M.P.; Roël, G.; Eichhorn, P.J.A.; Hijmans, E.M.; Maurer, I.; Destrée, O.; Bernards, R.A.

    2005-01-01

    The Wnt signaling cascade is a central regulator of cell fate determination during embryonic development, whose deregulation contributes to oncogenesis. Naked cuticle is the first Wnt-induced antagonist found in this pathway, establishing a negative-feedback loop that limits the Wnt signal required

  14. Virtually naked: virtual environment reveals sex-dependent nature of skin disclosure.

    Directory of Open Access Journals (Sweden)

    Anna M Lomanowska

    Full Text Available The human tendency to reveal or cover naked skin reflects a competition between the individual propensity for social interactions related to sexual appeal and interpersonal touch versus climatic, environmental, physical, and cultural constraints. However, due to the ubiquitous nature of these constraints, isolating on a large scale the spontaneous human tendency to reveal naked skin has remained impossible. Using the online 3-dimensional virtual world of Second Life, we examined spontaneous human skin-covering behavior unhindered by real-world climatic, environmental, and physical variables. Analysis of hundreds of avatars revealed that virtual females disclose substantially more naked skin than virtual males. This phenomenon was not related to avatar hypersexualization as evaluated by measurement of sexually dimorphic body proportions. Furthermore, analysis of skin-covering behavior of a population of culturally homogeneous avatars indicated that the propensity of female avatars to reveal naked skin persisted despite explicit cultural norms promoting less revealing attire. These findings have implications for further understanding how sex-specific aspects of skin disclosure influence human social interactions in both virtual and real settings.

  15. PR72, a novel regulator of Wnt signaling required for Naked cuticle function

    NARCIS (Netherlands)

    Creyghton, M.P.; Roël, G.; Eichhorn, P.J.A.; Hijmans, E.M.; Maurer, I.; Destrée, O.; Bernards, R.A.

    2005-01-01

    The Wnt signaling cascade is a central regulator of cell fate determination during embryonic development, whose deregulation contributes to oncogenesis. Naked cuticle is the first Wnt-induced antagonist found in this pathway, establishing a negative-feedback loop that limits the Wnt signal required

  16. Antibacterial Mechanism of The Ehyl Acetate Extracts From Naked Oat Again Bacillus subtilis

    Directory of Open Access Journals (Sweden)

    Li-Huan Yuan

    2015-03-01

    Full Text Available The antibacterial mechanism of the ethyl acetate extracts from naked oat against Bacillus subtilis were studied in this paper based on assays such as cell wall alkaline phosphatase (AKP, permeability and integrity of cell membrane as well as electron microscopy observations. The results showed that the ethyl acetate extracts had significant effects on AKP, permeability and integrity of cell membrane. We concluded that the mechanism of action of the ethyl acetate extracts against B. subtilis might be described as:Acting on cell wall and membrane, resulting in disruption, cell lysis, and the leakage of intracellular constituents according to the results of AKP, the leakage of electrolytes, the losses of contents (proteins, reducing sugars and 260 nm absorbing materials assays and electron microscopy observations. Overall, the results clearly indicated that the ethyl acetate extracts from naked oat was potential to control the contamination of foods caused by bacterial diseases

  17. A Naked Eye Refractive Index Sensor with a Visible Multiple Peak Metamaterial Absorber

    Directory of Open Access Journals (Sweden)

    Heli Ma

    2015-03-01

    Full Text Available We report a naked eye refractive index sensor with a visible metamaterial absorber. The visible metamaterial absorber consisting of a silver dendritic/dielectric/metal structure shows multiple absorption peaks. By incorporating a gain material (rhodamine B into the dielectric layer, the maximal magnitude of the absorption peak can be improved by about 30%. As the metamaterial absorber is sensitive to the refractive index of glucose solutions, it can function as a sensor that quickly responds to variations of the refractive index of the liquid. Meanwhile, since the response is presented via color changes, it can be clearly observed by the naked eyes. Further experiments have confirmed that the sensor can be used repeatedly.

  18. Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye

    Science.gov (United States)

    de La Rica, Roberto; Stevens, Molly M.

    2012-12-01

    In resource-constrained countries, affordable methodologies for the detection of disease biomarkers at ultralow concentrations can potentially improve the standard of living. However, current strategies for ultrasensitive detection often require sophisticated instruments that may not be available in laboratories with fewer resources. Here, we circumvent this problem by introducing a signal generation mechanism for biosensing that enables the detection of a few molecules of analyte with the naked eye. The enzyme label of an enzyme-linked immunosorbent assay (ELISA) controls the growth of gold nanoparticles and generates coloured solutions with distinct tonality when the analyte is present. Prostate specific antigen (PSA) and HIV-1 capsid antigen p24 were detected in whole serum at the ultralow concentration of 1 × 10-18 g ml-1. p24 was also detected with the naked eye in the sera of HIV-infected patients showing viral loads undetectable by a gold standard nucleic acid-based test.

  19. Total Magnification and Magnification Centroid Due to Strongly Naked Singularity Lensing

    CERN Document Server

    DeAndrea, Justin

    2015-01-01

    A strongly naked singularity (SNS) was modelled at the center of the Galaxy. This specific type of naked singularity was characterized in 2008 by Virbhadra and Keeton. The lens is described using the Janis-Newman-Winicour metric, which has an ordinary mass and massless scalar charge parameters. Gravitational lensing by the SNS gives rise to 4 images: 2 images on the same side as the source and 2 images on the opposite side of the source from the optic axis. We compute magnification centroid, magnification centroid shift, and total absolute magnification for many values of the angular source position. The nature of the curve for all three results are qualitatively similar to Schwarzschild black hole lenses, but quantitatively different. Magnification centroid increases as angular source position increases. As angular source position increases, magnification centroid shift increases to a maximum value, and then begins to decrease. As angular source position becomes large, magnification centroid shift will appro...

  20. Examples of naked singularity formation in higher-dimensional Einstein-vacuum spacetimes

    CERN Document Server

    An, Xinliang; Lü, H

    2015-01-01

    The vacuum Einstein equations in 5+1 dimensions are shown to admit solutions describing naked singularity formation in gravitational collapse from nonsingular asymptotically locally flat initial data. We present a class of specific examples with spherical extra dimensions. Thanks to the Kaluza-Klein dimensional reduction, these spacetimes are constructed by lifting continuously self-similar solutions of the 4-dimensional Einstein-scalar field system with a negative exponential potential. Their existence provides a new test-bed for weak cosmic censorship in higher-dimensional gravity. In addition, we point out why a similar attempt of embedding Christodoulou's well-known solutions for massless scalar fields fails to capture formation of naked singularities in 4+1 dimensions.

  1. Quantum Backreaction on Three-Dimensional Black Holes and Naked Singularities.

    Science.gov (United States)

    Casals, Marc; Fabbri, Alessandro; Martínez, Cristián; Zanelli, Jorge

    2017-03-31

    We analytically investigate backreaction by a quantum scalar field on two rotating Bañados-Teitelboim-Zanelli (BTZ) geometries: that of a black hole and that of a naked singularity. In the former case, we explore the quantum effects on various regions of relevance for a rotating black hole space-time. We find that the quantum effects lead to a growth of both the event horizon and the radius of the ergosphere, and to a reduction of the angular velocity, compared to the unperturbed values. Furthermore, they give rise to the formation of a curvature singularity at the Cauchy horizon and show no evidence of the appearance of a superradiant instability. In the case of a naked singularity, we find that quantum effects lead to the formation of a horizon that shields it, thus supporting evidence for the rôle of quantum mechanics as a cosmic censor in nature.

  2. Ultra-high energy particle collisions in a regular spacetime without blackholes or naked singularities

    CERN Document Server

    Patil, Mandar

    2012-01-01

    We investigate here the particle acceleration and collisions with extremely large center of mass energies in a perfectly regular spacetime containing neither singularity nor an event horizon. The ultra-high energy collisions of particles near the event horizon of extremal Kerr blackhole, and also in many other examples of extremal blackholes have been investigated and reported recently. We studied an analogous particle acceleration process in the Kerr and Reissner- Nordstrom spacetimes without horizon, containing naked singularities. Further to this, we show here that the particle acceleration and collision process is in fact independent of blackholes and naked singularities, and can happen in a fully regular spacetime containing neither of these. We derive the conditions on the general static spherically symmetric metric for such a phenomena to happen. We show that in order to have ultra-high energy collisions it is necessary for the norm of the timelike Killing vector to admit a maximum with a vanishingly s...

  3. Naked eye plasmonic indicator with multi-responsive polymer brush as signal transducer and amplifier.

    Science.gov (United States)

    Wang, Tieqiang; Yu, Ye; Chen, Dan; Wang, Shuli; Zhang, Xuemin; Li, Yunong; Zhang, Junhu; Fu, Yu

    2017-02-02

    For decades, plasmonic nanostructures have been used as important optical sensing platforms, however, the necessity of sensitive optical instruments for detection greatly limits their practical application. Herein, a multi-responsive naked eye plasmonic indicator has been prepared through introduction of a responsive polymer brush (PNIPAm) into the cavity of a Ag nanovolcano array (Ag NVA). According to the phase change of the PNIPAm brush under different external conditions, the as-prepared Ag NVA shows responsive monochromatic colors, which allow the Ag NVA to serve as a plasmonic indicator detected by the naked eye. Importantly, the as-prepared Ag NVA also possesses a rapid response rate as well as excellent repeatability, and is compatible with conventional micro-fabrication methods. All of these excellent features make the as-prepared Ag NVA an attractive candidate for future optical indicating and intelligent color display applications.

  4. Newtonian analogue of static general relativistic spacetimes: An extension to naked singularities

    CERN Document Server

    Ghosh, Shubhrangshu; Bhadra, Arunava

    2015-01-01

    We formulate a generic Newtonian like analogous potential for static spherically symmetric general relativistic (GR) spacetime, and subsequently derived proper Newtonian like analogous potential corresponding to Janis-Newman-Winicour (JNW) and Reissner-Nordstr\\"{o}m (RN) spacetimes, both exhibiting naked singularities. The derived potentials found to reproduce the entire GR features including the orbital dynamics of the test particle motion and the orbital trajectories, with precise accuracy. The nature of the particle orbital dynamics including their trajectory profiles in JNW and RN geometries show altogether different behavior with distinctive traits as compared to the nature of particle dynamics in Schwarzschild geometry. Exploiting the Newtonian like analogous potentials, we found that the radiative efficiency of a geometrically thin and optically thick Keplerian accretion disk around naked singularities corresponding to both JNW and RN geometries, in general, is always higher than that for Schwarzschild...

  5. Optical effects related to Keplerian discs orbiting Kehagias-Sfetsos naked singularities

    CERN Document Server

    Stuchlik, Zdenek

    2014-01-01

    We demonstrate possible optical signatures of the Kehagias-Sfetsos naked singularity spacetimes representing spherically symmetric vacuum solution of the modified Ho\\v{r}ava gravity. In such spacetimes, accretion structures significantly different from those present in the standard black hole spacetimes occur due to the "antigravity" effect causing existence of an internal static sphere surrounded by Keplerian discs. We focus our attention on the optical effects related to the Keplerian accretion discs, constructing the optical appearance of the Keplerian discs, the spectral continuum due to their thermal radiation, and spectral profiled lines generated in the innermost parts of such discs. The KS naked singularity signature is strongly encoded in the characteristics of predicted optical effects, especially in the case of the spectral continuum and spectral lines profiled by the strong gravity of the spacetimes, due to the region of the vanishing of the angular velocity gradient influencing the effectivity of...

  6. New Finds of Naked Amoebae (Protista in Water Reservoirs of Ukraine

    Directory of Open Access Journals (Sweden)

    Patsyuk M. K.

    2016-08-01

    Full Text Available In the water bodies of Ukraine, 6 new species of naked amoebae were found: Saccamoeba sp., Ripella sp., Vannella lata Page, 1988, The camoeba sp., Acanthamoeba sp., Vahlkampfia sp. According to the current taxonomy, they belong to 3 classes, 4 orders, 5 families and 6 genera. New localities and original descriptions of the species are presented, along with brief characteristics of the corresponding genera. The camoeba sp. and Acanthamoeba sp. are first reported from the territory of Ukraine.

  7. The “Naked Coral” Hypothesis Revisited – Evidence for and Against Scleractinian Monophyly

    Science.gov (United States)

    Forêt, Sylvain; Huttley, Gavin; Miller, David J.; Chen, Chaolun Allen

    2014-01-01

    The relationship between Scleractinia and Corallimorpharia, Orders within Anthozoa distinguished by the presence of an aragonite skeleton in the former, is controversial. Although classically considered distinct groups, some phylogenetic analyses have placed the Corallimorpharia within a larger Scleractinia/Corallimorpharia clade, leading to the suggestion that the Corallimorpharia are “naked corals” that arose via skeleton loss during the Cretaceous from a Scleractinian ancestor. Scleractinian paraphyly is, however, contradicted by a number of recent phylogenetic studies based on mt nucleotide (nt) sequence data. Whereas the “naked coral” hypothesis was based on analysis of the sequences of proteins encoded by a relatively small number of mt genomes, here a much-expanded dataset was used to reinvestigate hexacorallian phylogeny. The initial observation was that, whereas analyses based on nt data support scleractinian monophyly, those based on amino acid (aa) data support the “naked coral” hypothesis, irrespective of the method and with very strong support. To better understand the bases of these contrasting results, the effects of systematic errors were examined. Compared to other hexacorallians, the mt genomes of “Robust” corals have a higher (A+T) content, codon usage is far more constrained, and the proteins that they encode have a markedly higher phenylalanine content, leading us to suggest that mt DNA repair may be impaired in this lineage. Thus the “naked coral” topology could be caused by high levels of saturation in these mitochondrial sequences, long-branch effects or model violations. The equivocal results of these extensive analyses highlight the fundamental problems of basing coral phylogeny on mitochondrial sequence data. PMID:24740380

  8. On naked singularities in the extreme double Reissner-Nordstr\\"{o}m solution

    CERN Document Server

    Cabrera-Munguia, I

    2014-01-01

    We present a quite simple analytical study on the appearance or absence of naked singularities in binary systems. As an example we consider the double Reissner-Nordstr\\"om solution and fix the conditions it should satisfy in order to avoid or develop singular surfaces off the axis. The proof shows that singular surfaces appear as a consequence of the presence of negative masses in the solution.

  9. Analysis of the Prompt Optical Emission of the Naked-Eye GRB 080319B

    CERN Document Server

    Bartolini, C; Guarnieri, A; Piccioni, A; Beskin, G; Bondar, S; Karpov, S; Molinari, E

    2009-01-01

    We present the observed/intrinsic optical parameters and the variability analysis of the Naked-Eye Burst, GRB 080319B, observed by the TORTORA wide-field optical monitoring system. The event is extreme not only in observed properties but also intrinsically: it is the most luminous event ever recorded at optical wavelengths. The temporal properties suggest short-lived periodic activities of the internal engine. This is the fastest optically variable source detected at cosmological distances.

  10. The "naked coral" hypothesis revisited--evidence for and against scleractinian monophyly.

    Directory of Open Access Journals (Sweden)

    Marcelo V Kitahara

    Full Text Available The relationship between Scleractinia and Corallimorpharia, Orders within Anthozoa distinguished by the presence of an aragonite skeleton in the former, is controversial. Although classically considered distinct groups, some phylogenetic analyses have placed the Corallimorpharia within a larger Scleractinia/Corallimorpharia clade, leading to the suggestion that the Corallimorpharia are "naked corals" that arose via skeleton loss during the Cretaceous from a Scleractinian ancestor. Scleractinian paraphyly is, however, contradicted by a number of recent phylogenetic studies based on mt nucleotide (nt sequence data. Whereas the "naked coral" hypothesis was based on analysis of the sequences of proteins encoded by a relatively small number of mt genomes, here a much-expanded dataset was used to reinvestigate hexacorallian phylogeny. The initial observation was that, whereas analyses based on nt data support scleractinian monophyly, those based on amino acid (aa data support the "naked coral" hypothesis, irrespective of the method and with very strong support. To better understand the bases of these contrasting results, the effects of systematic errors were examined. Compared to other hexacorallians, the mt genomes of "Robust" corals have a higher (A+T content, codon usage is far more constrained, and the proteins that they encode have a markedly higher phenylalanine content, leading us to suggest that mt DNA repair may be impaired in this lineage. Thus the "naked coral" topology could be caused by high levels of saturation in these mitochondrial sequences, long-branch effects or model violations. The equivocal results of these extensive analyses highlight the fundamental problems of basing coral phylogeny on mitochondrial sequence data.

  11. Cosmic censorship and stationary states of half-spin particles in the field of Reissner-Nordstroem naked singularity

    CERN Document Server

    Gorbatenko, M V; Popov, E Yu; Safronov, I I

    2015-01-01

    The paper explores quantum mechanics of half-spin particle motion in the field of Reissner-Nordstroem (RN) naked singularity. It is shown that for any quantum mechanical Dirac particle, irrespective of availability and sign of its electrical charge, the RN naked singularity is separated by an infinitely high positive potential barrier. With like charges of a particle and the source of the RN naked singularity, near the origin there exists the second completely impenetrable potential barrier. It has been proved that in the field of the RN naked singularity, bound states of half-spin particles can exist. The conditions for appearance of such states were revealed and computations were performed to find energy eigenvalues and eigenfunctions.

  12. Ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes.

    Science.gov (United States)

    Zhang, Heng; Zhang, Yali; Lin, Yankui; Liang, Tongwen; Chen, Zhihua; Li, Jinfeng; Yue, Zhenfeng; Lv, Jingzhang; Jiang, Qing; Yi, Changqing

    2015-09-15

    In this study, a novel approach for ultrasensitive detection and rapid high-throughput identification of a panel of common foodborne pathogens with the naked eyes is presented. As a proof-of-concept application, a multiple pathogen analysis array is fabricated through immobilizing three specific polyT-capture probes which can respectively recognize rfbE gene (Escherichia coli O157:H7), invA gene (Salmonella enterica), inlA gene (Listeria monocytogenes) on the plastic substrates. PCR has been developed for amplification and labeling target genes of rfbE, invA, inlA with biotin. The biotinated target DNA is then captured onto the surface of plastic strips through specific DNA hybridization. The succeeding staining of biotinated DNA duplexes with avidin-horseradish peroxidise (AV-HRP) and biotinated anti-HRP antibody greatly amplifies the detectable signal through the multiple cycle signal amplification strategy, and thus realizing ultrasensitive and specific detection of the above three pathogens in food samples with the naked eyes. Results showed approximately 5 copies target pathogenic DNA could be detected with the naked eyes. This simple but very efficient colorimetric assay also show excellent anti-interference capability and good stability, and can be readily applied to point-of-care diagnosis.

  13. Collider Experiment with Kerr Naked Singularities to probe Ultra-high Energy Physics

    CERN Document Server

    Patil, Mandar

    2011-01-01

    We investigate here the particle acceleration by Kerr naked singularities and propose an efficient mechanism to construct a collider experiment to study the beyond standard model physics all the way upto Planck scale, in their environment. In this work we show that the center of mass energy of collision between two particles, dropped in from a finite but arbitrary large distance along the axis of symmetry,is arbitrarily large, provided the deviation of the angular momentum parameter from the mass is very small for a Kerr naked singularity. The collisions considered here are between particles, one of them ingoing and the other one being initially an ingoing particle, which later emerges as an outgoing particle, after it suffers a reflection from a spatial region with repulsive gravity in the vicinity of the naked singularity. The chosen location for collisions marks a transition between attractive and repulsive regimes of gravity. Thus we argue that this would be an ideal site for the construction of a particl...

  14. Gis-Based Crop Support System For Common Oatand Naked Oat in China

    Science.gov (United States)

    Wan, Fan; Wang, Zhen; Li, Fengmin; Cao, Huhua; Sun, Guojun

    The identification of the suitable areas for common oat (Avena sativa L.) and naked oat (Avena nuda L.) in China using Multi-Criteria Evaluation (MCE) approach based on GIS is presented in the current article. Climate, topography, soil, land use and oat variety databases were created. Relevant criteria,suitability levels and their weights for each factor were defined. Then the criteria maps were obtained and turned into the MCE process, and suitability maps for common oat and naked oat were created. The land use and the suitability maps were crossed to identify the suitable areas for each crop. The results identified 397,720 km2 of suitable areas for common oats of forage purpose distributed in 744 counties in 17 provinces, and 556,232 km2 of suitable areas for naked oats of grain purpose distributed in 779 counties in 19 provinces. This result is in accordance with the distribution of farmingpastoral ecozones located in semi-arid regions of northern China. The mapped areas can help define the working limits and serve as indicative zones for oat in China. The created databases, mapped results, interface of expert system and relevant hardware facilities could construct a complete crop support system for oats.

  15. Optical effects related to Keplerian discs orbiting Kehagias-Sfetsos naked singularities

    Science.gov (United States)

    Stuchlík, Zdeněk; Schee, Jan

    2014-10-01

    We demonstrate possible optical signatures of the Kehagias-Sfetsos (KS) naked singularity spacetimes representing a spherically symmetric vacuum solution of the modified Hořava gravity. In such spacetimes, accretion structures significantly different from those present in standard black hole spacetimes occur due to the ‘antigravity’ effect, which causes an internal static sphere surrounded by Keplerian discs. We focus our attention on the optical effects related to the Keplerian accretion discs, constructing the optical appearance of the Keplerian discs, the spectral continuum due to their thermal radiation, and the spectral profiled lines generated in the innermost parts of such discs. The KS naked singularity signature is strongly encoded in the characteristics of predicted optical effects, especially in cases where the spectral continuum and spectral lines are profiled by the strong gravity of the spacetimes due to the vanishing region of the angular velocity gradient influencing the effectiveness of the viscosity mechanism. We can conclude that optical signatures of KS naked singularities can be well distinguished from the signatures of standard black holes.

  16. A Cyanine Dye Encapsulated Porous Fibrous Mat for Naked-Eye Ammonia Sensing.

    Science.gov (United States)

    Ji, Chendong; Ma, Lijing; Yin, Meizhen; Yang, Wantai; Pan, Kai

    2016-08-19

    Electrospun ultrathin fiber-based sensors are desirable because of their practicality and sensitivity. Ammonia-detection systems are in high demand in different areas, including the industrial and agricultural fields. However, current technologies rely on large and complex instruments that restrict their actual utilization. Herein, we report a flexible naked-eye ammonia sensor, the polylactic acid-cyanine (PLA-Cy) fibrous mat, which was fabricated by blending a carboxyl-functionalized cyanine dye (D1) into electospun PLA porous fibers. The sensing mat was shown to undergo a naked-eye-detectable color change from white to blue upon exposure to ammonia vapor. The mat showed high selectivity to ammonia gas with a detection limit of 3.3 ppm. Aggregated D1 was first encapsulated by PLA and was then ionized by NH3 . These mechanisms were examined by photophysical studies and scanning electron microscopy. The aggregation-deaggregation process of D1 in the PLA-Cy fibrous mat led to the color change. This work provides a facile method for the naked-eye detection of ammonia and a novel strategy for the use of organic dyes in ammonia sensing.

  17. CONTRIBUTIONS TO KNOWING THE SEGMENTED MESODERM DEVELOPMENT IN TRANSYLVANIAN NAKED NECK BREED

    Directory of Open Access Journals (Sweden)

    D. DRONCA

    2013-07-01

    Full Text Available In Romania, the Transylvanian Naked Neck hen breed is considered to be an “endangered” population, reason for which we consider that a special attention should have been given then until now. Plymouth Rock breed was imported for the first time to Romania from the Studler Company, France in 1969. The present work is aiming to study profoundly the development model for development at 26, 30, 40, 50 and 60 hours incubation age, in Transylvanian Naked Neck hen breed and compare it to Plymouth Rock breed. The observations are proving that in the first 60 incubation hours the segmented mesoderm morphogenesis in the two breed, is different, dictated by the two different genetic structures. It is noticeable that Transylvanian Naked Neck is characterized by a vigorous embryonic development, overcoming, the other breed tested by the age of 40 incubation hours. We estimate that the two breeds have a good combinative capacity, which recommend the utilization of these genetic materials to obtain hybrids for producing “peasant-type” chicken meat, very well-appreciated by the Europeans between the two World Wars.

  18. Genetic Similarity Assessment among Selected Naked Oat Cultivars and Breeding Lines Using ISSR Markers

    Directory of Open Access Journals (Sweden)

    Edyta PACZOS-GRZEDA

    2014-06-01

    Full Text Available Naked oat refers to a variety of Avena sativa with lemma and palea separating from the grains. Its spikelets are multiflorous and morphologically different from the husked oat. Problems with preharvest sprouting, threshability, rancidity, a wide range of kernel sizes, as well as its relatively low tolerance to limited soil water content, are its main drawbacks. Nevertheless it could be an alternative to a conventional oat. Unfortunately, its genetic variation is still poorly recognized.  In the given study a set of 26 naked oat cultivars and lines were analyzed with 25 inter-simple sequence repeats (ISSR primers that amplified as many as 429 DNA fragments among which 204 were polymorphic. The average number of markers amplified per primer pair and polymorphism information content (PIC value equaled to eight and 0.23, respectively. Forty four unique PCR products were identified for different genotypes. While Unweighted Pair-Group Method with Arithmetic Mean failed to distinguish the materials into main clusters it demonstrated that cultivars ‘Akt’, ‘Polar’, ‘Cacko’, ‘Siwek’, ‘Nagus’ and most of the DC lines were within a single group. Moreover, the cultivars that were closely related based on their breeding pedigree (related to ‘Akt’ were close to each other. Principal Coordinate Analysis explained 54.1% of variance and was in good agreement with the UPGMA. ISSR markers could be used for the evaluation of genetic similarity of cultivars and lines as well as the differentiation of individual genotypes. This study demonstrated that the available A. sativa naked type genetic pool is relatively wide and have the potential for further breeding progress.

  19. A Novel Approach of Low-frequency Ultrasonic Naked Plasmid Gene Delivery and Its Assessment

    Institute of Scientific and Technical Information of China (English)

    WEI WANG; ZHENG-ZHONG BIAN; YONG-JIE WU; YA-LIN MIAO

    2005-01-01

    Objective To deliver the naked genes into cells through the bioeffects of cell membrane porous produced by low-frequency ultrasound (US) and to investigate the safety by determining the threshold of cell damage and membrane permeability. Methods The suspension of red cells from chickens, rabbits, rats, and S180 cells was exposed to calibrated US field with different parameters in still and flowing state. Laser scanning confocal microscopy, fluorescent microscopy, scanning electron microscopy, flow cytometry and spectrophotometry were used to examine cell morphology, membrane permeability, enzymes, free radicals, naked gene expression efficiency, threshold of cell damage and cell viability. Results The plasmid of green fluorescent protein (GFP) as a reporter gene was delivered into S180 cells under optimal conditions without cell damage and cytotoxicity. The transfection rate was (35.83±2.53)% (n=6) in viable cells, and the cell viability was (90.17±1.47)% (n=6). Also, malondialdehyde, hydroxyl free radical, alkaline phosphatase, and acid phosphatase showed a S-shaped growth model (r=0.98±0.01) in response to the permeability change and alteration of cell morphology. The constant E of energy accumulation in US delivery at 90% cell viability was an optimal control factor, and at 80% cell viability was the damage threshold. Conclusion US under optimal conditions is a versatile gene therapy tool. The intensity of GFP expression in US group has a higher fluorescent peak than that in AVV-GFP group and control group (P<0.001). The optimal gene uptakes, expression of gene and safety depend on E, which can be applied to control gene delivery efficiency in combination with other parameters. The results are helpful for development of a novel clinical naked gene therapeutic system and non-hyperthermia cancer therapeutic system.

  20. Review roundtable: naked diplomacy: power and statecraft in the digital age by Tom Fletcher

    OpenAIRE

    Kitchen, Nicholas; Wichowski, Alexis; Khatib, Lina; Neumann, Iver B.; Murabit, Alaa; Kelley, Robert

    2016-01-01

    What is the role of the diplomat in the digital age? In Naked Diplomacy: Power and Statecraft in the Digital Age, Tom Fletcher – former British ambassador to Lebanon and the youngest to be appointed in 200 years – draws upon his own experiences to outline a progressive vision of contemporary diplomacy that challenges top-down or one-way models of communication between diplomats and citizens to instead focus on direct engagement through the use of tools such as social media. In this review rou...

  1. Naked eye picometer resolution in a Michelson interferometer using conjugated twisted beams.

    Science.gov (United States)

    Emile, Olivier; Emile, Janine

    2017-01-15

    Michelson interferometry is one of the most widely used techniques for accuracy measurements. Its main characteristic feature is to infer a displacement in one of the arms of the interferometer from a phase measurement. Two different twisted beams, also called vortex beams, with opposite twisted rotations in each arm of the interferometer interfere in a daisy flower-like pattern. The number of petals is twice the topological charge. Their position depends on the relative phase of the beams. Naked eye detection of 44 pm displacements is achieved. The sensitivity of such an interferometer together with possible further improvements, and applications are then discussed.

  2. Axially symmetric, asymptotically flat vacuum metric with a naked singularity and closed timelike curves

    CERN Document Server

    Sarma, Debojit; Patgiri, Mahadev

    2016-01-01

    We present an axially symmetric, asymptotically flat empty space solution of the Einstein field equations containing a naked singularity. The spacetime is regular everywhere except on the symmetry axis where it possess a true curvature singularity. The spacetime is of type D in the Petrov classification scheme and is locally isometric to the metrics of case IV in the Kinnersley classification of type D vacuum metrics. Additionally, the spacetime also shows the evolution of closed timelike curves (CTCs) from an initial hypersurface free from CTCs.

  3. Spatiotemporal multiplexing method for visual field of view extension in holographic displays with naked eye observation

    Science.gov (United States)

    Finke, G.; Kujawińska, M.; Kozacki, T.; Zaperty, W.

    2016-09-01

    In this paper we propose a method which allows to overcome the basic functional problems in holographic displays with naked eye observation caused by delivering too small images visible in narrow viewing angles. The solution is based on combining the spatiotemporal multiplexing method with a 4f optical system. It enables to increase an aperture of a holographic display and extend the angular visual field of view. The applicability of the modified display is evidenced by Wigner distribution analysis of holographic imaging with spatiotemporal multiplexing method and by the experiments performed at the display demonstrator.

  4. A preliminary phylogeny of the Palearctic naked-toed geckos (Reptilia: Squamata: Gekkonidae) with taxonomic implications.

    Science.gov (United States)

    Bauer, Aaron M; Masroor, Rafaqat; Titus-McQuillan, James; Heinicke, Matthew P; Daza, Juan D; Jackman, Todd R

    2013-01-08

    Palearctic naked-toed geckos are a group of gekkonid geckos that range from North Africa to northern India and western China, with their greatest diversity in Iran and Pakistan. Relationships among the constituent genera remain incompletely resolved and the monophyly of key genera remains unverified. Further, competing classifications are in current use and many species have been allocated to different genera by different authors. We used both mitochondrial (ND2) and nuclear genes (RAG1, PDC) to explore relationships among representatives of all but one genus in the group (Rhinogecko), including four genera not previously included in phylogenetic analyses (Asiocolotes, Altigekko, Indogekko, and Siwaligekko). Siwaligekko (and presumably other Tibeto-Himalayan species often referred to Cyrtopodion) are more closely related to tropical Asian Cyrtodactylus than to Palearctic naked-toed geckos. Sampled species of Asiocolotes and Altigekko are sister taxa, but both genera are here considered junior subjective synonyms of Altiphylax. Cyrtopodion sensu lato is non-monophyletic; Mediodactylus and Tenuidactylus, which have variably been considered as subgenera or synonyms of Cyrtopodion are both valid genera. Indogekko is embedded within Cyrtopodion and is here treated as a subgenus. Bunopus and Crossobamon are closely related to one-another, and with Agamura are interdigitated among taxa previously assigned to Cyrtopodion. Our data confirm the previous identification of a Saharo-Arabian Stenodactylus/Tropiocolotes/Pseudoceramodactylus clade and verify that Microgecko and Alsophylax are not members of the main clade of Palearctic naked-toed geckos. Osteological differences between Tropiocolotes and Microgecko, formerly treated as congeneric, are discussed and illustrated. The divergence between Cyrtodactylus and the Palearctic naked-toed clade predates the initial collision of the Indian and Eurasian plates, but deeper divergences within both groups are

  5. Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides.

    Science.gov (United States)

    Verga, Daniela; Welter, Moritz; Marx, Andreas

    2016-02-01

    DNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.

  6. Label-free silver nanoparticles for the naked eye detection of entecavir

    Science.gov (United States)

    Gao, Mengmeng; Lin, Rui; Li, Lili; Jiang, Li; Ye, Baofen; He, Hua; Qiu, Lanlan

    A simple, rapid, field-portable colorimetric method for the detection of entecavir was proposed based on the color change caused by the aggregation of silver nanoparticles. Neutralization of the electrostatic repulsion from each silver nanoparticle resulted in the aggregation of AgNPs and a consequent color change of AgNPs from yellow to wine-red, which provided a platform for rapid and field-portable colorimetric detection of entecavir. The concentration of entecavir could be determined with naked eye or UV-vis spectrometer. The proposed method can be used to detect entecavir in human urine with a detection limit of 1.51 μg mL-1, within 25 min by naked eye observation without the aid of any advanced instrument or complex pretreatment. Results from UV-vis spectra showed that the absorption ratio was linear with the concentration of entecavir in the range of 5.04-25.2 μg mL-1 and 1.01-5.04 μg mL-1 with linear coefficients of 0.9907 and 0.9955, respectively. The selectivity of AgNPs detection system for entecavir is excellent comparing with other ions and analytes. Due to its rapid, visible color changes, and excellent selectivity, the AgNPs synthesized in this study are suitable to be applied to on-site screening of entecavir in human urine.

  7. FARINOGRAPHIC EVALUATION OF WHEAT DOUGH ENRICHED WITH INULIN, NAKED BARLEY, MALT AND POTEX ADDITION

    Directory of Open Access Journals (Sweden)

    Marián Tokár

    2015-02-01

    Full Text Available One of the most important biological substances consumed in insufficient quantity is fibre that is often deficient in the diet. In general, dietary fibre is edible part of plants, or similar carbohydrates, that are resistant to digestion and absorption in the small intestine. Nowadays, there are many sources of fibre available for bakery industry, whether through addition of non-traditional bread cereals or through isolated form as additives. Except to supposed increase the nutritional value of bakery products with added fiber and raw materials containing fiber is necessary to think about their technological quality and rheological properties of dough. The aim of this experimental work was to investigate the effect of the addition of selected polysaccharides (inulin, Potex - potato fiber and non-bakery crops (naked barley, malt containing significant polysaccharides used in the mixture with wheat flour type T-650 in different ratios on the basis of farinographic evaluations. Based on the results of rheological measurements we found out that quality of dough was deteriorating proportionally to the amount of used additives. On the other hand, positively could be considered the increase of water absorption with addition of Potex and naked barley.

  8. Naked-eye quantitative aptamer-based assay on paper device.

    Science.gov (United States)

    Zhang, Yun; Gao, Dong; Fan, Jinlong; Nie, Jinfang; Le, Shangwang; Zhu, Wenyuan; Yang, Jiani; Li, Jianping

    2016-04-15

    This work initially describes the design of low-cost, naked-eye quantitative aptamer-based assays by using microfluidic paper-based analytical device (μPAD). Two new detection motifs are proposed for quantitative μPAD measurement without using external electronic readers, which depend on the length of colored region in a strip-like μPAD and the number of colorless detection microzones in a multi-zone μPAD. The length measuring method is based on selective color change of paper from colorless to blue-black via formation of iodine-starch complex. The counting method is conducted on the basis of oxidation-reduction reaction between hydrogen peroxide and potassium permanganate. Their utility is well demonstrated with sensitive, specific detection of adenosine as a model analyte with the naked eye in buffer samples and undiluted human serum. These equipment-free quantitative methods proposed thus hold great potential for the development of more aptamer-based assays that are simple, cost-efficient, portable, and user-friendly for various point-of-care applications particularly in resource-constrained environments.

  9. Productive performance of naked neck chickens that were fed leaf meal shrubs

    Directory of Open Access Journals (Sweden)

    Santos M Herrera G.

    2015-12-01

    Full Text Available Objective. To compare the productive performance of naked neck chickens (phases of initiation, growth and final that were fed meals Gliricidia sepium, Cajanus cajan and Morus alba leaves. Materials and methods. 192 chickens, 1-84 days of age were distributed in a randomized block design with three experimental groups (5% of shrub in the diet, 48 animals/ group, eight replicates/ treatment, six animals/ reply and three animals/ sex in each replicate were used. The control group consumed diet based on corn and soybeans. They were reared on floor. Weighed every seven days. Weight gain, voluntary intake, conversion, balance and efficiency of feed utilization were calculated. Results. The highest total feed intake and average daily gain in rearing were 37.43g 9509.96 g respectively for M. alba (p<0.05, which also presented the best efficiency of energy and protein. Meanwhile, G. sepium showed the lowest values. Conclusions. It is possible to replace 5% of corn and soy in the diet of naked neck chickens, with the inclusion of leaf meal M. alba and get a favorable productive behavior.

  10. Micrometer-level naked-eye detection of caesium particulates in the solid state

    Directory of Open Access Journals (Sweden)

    Taizo Mori, Masaaki Akamatsu, Ken Okamoto, Masato Sumita, Yoshitaka Tateyama, Hideki Sakai, Jonathan P Hill, Masahiko Abe and Katsuhiko Ariga

    2013-01-01

    Full Text Available Large amounts of radioactive material were released from the Fukushima Daiichi nuclear plant in Japan, contaminating the local environment. During the early stages of such nuclear accidents, iodine I-131 (half-life 8.02 d is usually detectable in the surrounding atmosphere and bodies of water. On the other hand, in the long-term, soil and water contamination by Cs-137, which has a half-life of 30.17 years, is a serious problem. In Japan, the government is planning and carrying out radioactive decontamination operations not only with public agencies but also non-governmental organizations, making radiation measurements within Japan. If caesium (also radiocaesium could be detected by the naked eye then its environmental remediation would be facilitated. Supramolecular material approaches, such as host–guest chemistry, are useful in the design of high-resolution molecular sensors and can be used to convert molecular-recognition processes into optical signals. In this work, we have developed molecular materials (here, phenols as an optical probe for caesium cation-containing particles with implementation based on simple spray-on reagents and a commonly available fluorescent lamp for naked-eye detection in the solid state. This chemical optical probe provides a higher spatial resolution than existing radioscopes and gamma-ray cameras.

  11. FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines.

    Science.gov (United States)

    Kreiter, Sebastian; Diken, Mustafa; Selmi, Abderraouf; Petschenka, Jutta; Türeci, Özlem; Sahin, Ugur

    2016-01-01

    Intranodal immunization with antigen-encoding naked mRNA has proven to be an efficacious and safe approach to induce antitumor immunity. Thanks to its unique characteristics, mRNA can act not only as a source for antigen but also as an adjuvant for activation of the immune system. The search for additional adjuvants that can be combined with mRNA to further improve the potency of the immunization revealed Fms-like tyrosine kinase 3 (FLT3) ligand as a potent candidate. Systemic administration of the dendritic cell-activating FLT3 ligand prior to or along with mRNA immunization-enhanced priming and expansion of antigen-specific CD8(+) T cells in lymphoid organs, T-cell homing into melanoma tumors, and therapeutic activity of the intranodally administered mRNA. Both compounds demonstrate a successful combination in terms of boosting the immune response. This chapter describes methods for intranodal immunization with naked mRNA by co-administration of FLT3 ligand, which leads to strong synergistic effects.

  12. Finite escape fraction for ultrahigh energy collisions around Kerr naked singularity

    Indian Academy of Sciences (India)

    Mandar Patil; Pankaj S Joshi

    2015-04-01

    We investigate the issue of observability of high-energy collisions around Kerr naked singularity and show that results are in contrast with the Kerr black hole case. We had shown that it would be possible to have ultrahigh energy collisions between the particles close to the location = M around the Kerr naked singularity if the Kerr spin parameter transcends unity by an infinitesimally small amount → 1+. The collision is between initially ingoing particle that turns back as an outgoing particle due to angular momentum barrier, with another ingoing particle. We assume that two massless particles are produced in such a collision and their angular distribution is isotropic in the centre-of-mass frame. We calculated the escape fraction for the massless particles to reach infinity. We showed that the escape fraction is finite and approximately equal to half for the ultrahigh energy collisions. Therefore, the particles produced in high-energy collisions would escape to infinity providing the signature of the nature of basic interactions at those energies. This result is in contrast with the case of extremal Kerr black hole where almost all particles produced in high-energy collisions are absorbed by the black hole rendering collisions unobservable.

  13. Naked-eye fingerprinting of single nucleotide polymorphisms on psoriasis patients

    Science.gov (United States)

    Valentini, Paola; Marsella, Alessandra; Tarantino, Paolo; Mauro, Salvatore; Baglietto, Silvia; Congedo, Maurizio; Paolo Pompa, Pier

    2016-05-01

    We report a low-cost test, based on gold nanoparticles, for the colorimetric (naked-eye) fingerprinting of a panel of single nucleotide polymorphisms (SNPs), relevant for the personalized therapy of psoriasis. Such pharmacogenomic tests are not routinely performed on psoriasis patients, due to the high cost of standard technologies. We demonstrated high sensitivity and specificity of our colorimetric test by validating it on a cohort of 30 patients, through a double-blind comparison with two state-of-the-art instrumental techniques, namely reverse dot blotting and sequencing, finding 100% agreement. This test offers high parallelization capabilities and can be easily generalized to other SNPs of clinical relevance, finding broad utility in diagnostics and pharmacogenomics.We report a low-cost test, based on gold nanoparticles, for the colorimetric (naked-eye) fingerprinting of a panel of single nucleotide polymorphisms (SNPs), relevant for the personalized therapy of psoriasis. Such pharmacogenomic tests are not routinely performed on psoriasis patients, due to the high cost of standard technologies. We demonstrated high sensitivity and specificity of our colorimetric test by validating it on a cohort of 30 patients, through a double-blind comparison with two state-of-the-art instrumental techniques, namely reverse dot blotting and sequencing, finding 100% agreement. This test offers high parallelization capabilities and can be easily generalized to other SNPs of clinical relevance, finding broad utility in diagnostics and pharmacogenomics. Electronic supplementary information (ESI) available. See DOI: 10.1039/c6nr02200f

  14. A novel opal closest-packing photonic crystal for naked-eye glucose detection.

    Science.gov (United States)

    Hong, Xiaodi; Peng, Yuan; Bai, Jialei; Ning, Baoan; Liu, Yuanyuan; Zhou, Zhijiang; Gao, Zhixian

    2014-04-09

    A novel opal closest-packing (OCP) photonic crystal (PC) is successfully prepared for naked-eye glucose detection. This PC is fabricated via a vertical convective self-assembly method with a new type of monodisperse microsphere polymerized by co-monomers, namely, methyl methacrylate (MMA), N-isopropylacrylamide (NIPA), and 3-acrylamidophenylboronic acid (AAPBA). The OCP PC has high stability and periodically-ordered structure, showing the desired structural color. The proposed PC material displays a red shift and reduced reflection intensity when detecting glucose molecules. The red-shift wavelength reaches 75 nm, which clearly changes the structural color from brilliant blue to emerald green. This visually distinguishable color change facilitates the detection of the glucose concentrations from 3 to 20 mm, which demonstrates the potential of the opal PC material for naked-eye detection. Thus, the novel PMMA–NIPA–AAPBA OCP PC is a simply prepared and sensitive material, which shows promising use in the diagnosis of diabetes mellitus and in real-time monitoring of diabetes. Different types of appropriated recognition groups are expected to be introduced into the 3D OCP PC to form new functional materials or chemical sensors, which will extensively broaden the PC material application.

  15. Phylogeny of novel naked Filose and Reticulose Cercozoa: Granofilosea cl. n. and Proteomyxidea revised.

    Science.gov (United States)

    Bass, David; Chao, Ema E-Y; Nikolaev, Sergey; Yabuki, Akinori; Ishida, Ken-Ichiro; Berney, Cédric; Pakzad, Ursula; Wylezich, Claudia; Cavalier-Smith, Thomas

    2009-02-01

    Naked filose and reticulose protozoa were long lumped as proteomyxids or left outside higher groups. We cultivated eight naked filose or reticulose strains, did light microscopy, 18S rDNA sequencing and phylogeny (showing all are Cercozoa), and sequenced 80 environmental 18S-types. Filose species belong in subphylum Filosa and reticulose ones in subphylum Endomyxa, making proteomyxids polyphyletic. We therefore transfer the classically mainly reticulose Proteomyxidea to Endomyxa, removing evident filosans as new class Granofilosea (including Desmothoracida, Acinetactis and new heliomonad family Heliomorphidae (new genus Heliomorpha (=Dimorpha)). Five new species of Limnofila gen. n. (L. mylnikovi; L. anglica; L. longa; L. oxoniensis; L. borokensis, previously misidentified as Biomyxa (=Gymnophrys) cometa) form a large freshwater clade (new order Limnofilida). Mesofila limnetica gen., sp. n. and Nanofila marina gen., sp. n. group separately in Granofilosea (Cryptofilida ord. n.). In Endomyxa, a new genus of reticulose proteomyxids (Filoreta marina, F. japonica, F. turcica spp. n., F. (=Corallomyxa) tenera comb. n.) forms a clade (Reticulosida) related to Gromiidea/Ascetosporea. Platyreta germanica gen., sp. n. and Arachnula impatiens are related vampyrellids (Aconchulinida) within a large clade beside Phytomyxea. Biomyxidae and Rhizoplasmidae fam. n. remain incertae sedis within Proteomyxidea. Gymnophrydium and Borkovia are revised. The reticulose Corallomyxa are unlike Filoreta and possibly Amoebozoa, not Cercozoa.

  16. Motion of test particles in the field of a naked singularity

    Science.gov (United States)

    Boshkayev, K.; Gasperín, E.; Gutiérrez-Piñeres, A. C.; Quevedo, H.; Toktarbay, S.

    2016-01-01

    We investigate the motion of test particles in the gravitational field of a static naked singularity generated by a mass distribution with a quadrupole moment. We use the quadrupole metric (q metric) which is the simplest generalization of the Schwarzschild metric with a quadrupole parameter. We study the influence of the quadrupole on the motion of massive test particles and photons and show that the behavior of the geodesics can drastically depend on the values of the quadrupole parameter. In particular, we prove explicitly that the perihelion distance depends on the value of the quadrupole. Moreover, we show that an accretion disk on the equatorial plane of the quadrupole source can be either continuous or discrete, depending on the value of the quadrupole. The inner radius of the disk can be used in certain cases to determine the value of the quadrupole parameter. The case of a discrete accretion is interpreted as due to the presence of repulsive gravity generated by the naked singularity. Radial geodesics are also investigated and compared with the Schwarzschild counterparts.

  17. Motion of test particles in the field of a naked singularity

    CERN Document Server

    Boshkayev, K; Gutierrez-Pineres, A C; Quevedo, H; Toktarbay, S

    2016-01-01

    We investigate the motion of test particles in the gravitational field of a static naked singularity generated by a mass distribution with quadrupole moment. We use the quadrupole-metric ($q-$metric) which is the simplest generalization of the Schwarzschild metric with a quadrupole parameter. We study the influence of the quadrupole on the motion of massive test particles and photons and show that the behavior of the geodesics can drastically depend on the values of the quadrupole parameter. In particular, we prove explicitly that the perihelion distance depends on the value of the quadrupole. Moreover, we show that an accretion disk on the equatorial plane of the quadrupole source can be either continuous or discrete, depending on the value of the quadrupole. The inner radius of the disk can be used in certain cases to determine the value of the quadrupole parameter. The case of a discrete accretion is interpreted as due to the presence of repulsive gravity generated by the naked singularity. Radial geodesic...

  18. Script N = 4 Super Yang Mills at Finite Density: the Naked Truth

    Science.gov (United States)

    Evans, Nick; Hockings, James

    2002-07-01

    We study Script N = 4 super Yang Mills theory at finite U(1)R charge density (and temperature) using the AdS/CFT Correspondence. The ten dimensional backgrounds around spinning D3 brane configurations split into two classes of solution. One class describe spinning black branes and have previously been extensively studied, and interpreted, in a thermodynamic context, as the deconfined high density phase of the dual field theory. The other class have naked singularities and in the supersymmetric limit are known to correspond to multi-centre solutions describing the field theory in the Coulomb phase. We provide evidence that the non-supersymmetric members of this class represent naked, spinning D-brane distributions describing the Coulomb branch at finite density. At a critical density a phase transition occurs to a spinning black brane representing the deconfined phase where the Higgs vevs have evaporated. We perform a free energy calculation to determine the phase diagram of the Coulomb branch at finite temperature and density.

  19. N=4 Super Yang Mills at Finite Density the Naked Truth

    CERN Document Server

    Evans, N; Evans, Nick; Hockings, James

    2002-01-01

    We study N=4 super Yang Mills theory at finite U(1)_R charge density (and temperature) using the AdS/CFT Correspondence. The ten dimensional backgrounds around spinning D3 brane configurations split into two classes of solution. One class describe spinning black holes and have previously been extensively studied, and interpreted, in a thermodynamic context, as the deconfined high density phase of the dual field theory. The other class have naked singularities and in the supersymmetric limit are known to correspond to multi-centre solutions describing the field theory in the Coulomb phase. We provide evidence that the non-supersymmetric members of this class represent naked, spinning D-brane distributions describing the Coulomb branch at finite density. At a critical density a phase transition occurs to a spinning black hole representing the deconfined phase where the higgs vevs have evaporated. We perform a free energy calculation to determine the phase diagram of the Coulomb branch at finite temperature and ...

  20. Evaluating the Age-Dependent Potential for Protein Deposition in Naked Neck Meat Type Chicken

    Directory of Open Access Journals (Sweden)

    Daulat R. Khan

    2015-01-01

    Full Text Available The introduction of the naked neck gene (Na into modern meat type chicken is known to be helpful in increasing the tolerance for a high ambient temperature (AT by reducing the feather coverage which allows for a higher level of heat dissipation compared to normally feathered (na/na birds. In addition, reduced feather coverage could affect requirements for sulfur containing amino acids. As a prerequisite for further modeling of individual amino acid requirements, the daily N maintenance requirement (NMR and the threshold value of daily N retention (NRmaxT were determined. This was carried out using graded dietary protein supply and exponential modeling between N intake (NI and N excretion (NEX or N deposition (ND, respectively. Studies with homozygous (Na/Na and heterozygous (Na/na naked neck meat type chicken utilized 144 birds of average weight (50% of each genotype and sex within two N balance experiments during both the starter (days 10–20 and the grower period (days 25–35. Birds were randomly allotted to five diets with graded dietary protein supply but constant protein quality. The observed estimates depending on genotype, sex and age varied for NMR and NRmaxT from 224 to 395 and 2881 to 4049 mg N/BWkg0.67/day, respectively.

  1. Naked singularity and black hole formation in self-similar Einstein-scalar fields with exponential potentials

    CERN Document Server

    Zhang, Xuefeng

    2015-01-01

    Motivated by cosmic censorship in general relativity and string theory, we extend Christodoulou's celebrated examples of naked singularity formation in the Einstein-massless scalar field system to include a positive or negative scalar potential of exponential forms, i.e., $V(\\phi)=\\pm\\exp(2\\phi/\\kappa)$ with a parameter $\\kappa$. Under spherical symmetry and a self-similar ansatz depending on $\\kappa$, we derive a 3-dimensional autonomous system of first-order ordinary differential equations, which incorporates the equations for massless scalar fields as a special case. Local behavior of the phase space is studied analytically with global solutions constructed numerically. Within the 3-dimensional solution manifold, we observe, for the negative potentials, naked singularity formation from nonsingular initial data for $\\kappa^2<1$. Meanwhile, transitions between solutions containing naked singularities and black holes are also identified. However, when the potential is taken positive, numerical evolutions r...

  2. A new rhodamine-based colorimetric chemosensor for naked-eye detection of Cu(2+) in aqueous solution.

    Science.gov (United States)

    Hu, Yang; Zhang, Jing; Lv, Yuan-Zheng; Huang, Xiao-Huan; Hu, Sheng-li

    2016-03-15

    A new colorimetric probe 1 based on rhodamine B lactam was developed for naked-eye detection of Cu(2+). The optical feature of 1 for Cu(2+) was investigated by UV-vis absorption spectroscopy. Upon the addition of Cu(2+), the 1 displayed a distinct color change from colorless to pink, which can be directly detected by the naked eye. The stoichiometry of 1 to Cu(2+) complex was found to be 1:1 and the naked-eye detection limit was determined as low as 2 μM. The results suggest that the probe 1 may provide a convenient method for visual detection of Cu(2+) with high sensitivity.

  3. The efficacy of methylene blue encapsulated in silica nanoparticles compared to naked methylene blue for photodynamic applications.

    Science.gov (United States)

    Makhadmeh, Ghaseb Naser; Abdul Aziz, Azlan; Abdul Razak, Khairunisak

    2016-05-01

    This study analyzed the physical effects of methylene blue (MB) encapsulated within silica nanoparticles (SiNPs) in photodynamic therapy. The optimum concentration of MB needed to destroy red blood cells (RBCs) was determined, and the efficacy of encapsulated MB-SiNPs compared to that of naked MB was verified. The results confirmed the applicability of MB encapsulated in SiNPs on RBCs, and established a relationship between the concentration of the SiNP-encapsulated MB and the time required to rupture 50% of the RBCs (t50). The MB encapsulated in SiNPs exhibited higher efficacy compared to that of naked MB.

  4. Starch granule protein (SGP) polymorphism in cultivated naked barley from Qinghai-Tibet Plateau in China and relationship between SGPs and starch/amylose content

    Institute of Scientific and Technical Information of China (English)

    Zhifen PAN; Yixing ZOU; Tao ZHAO; Guangbing DENG; Xuguang ZHAI; Fang WU; Maoqun YU

    2008-01-01

    Starch granule proteins (SGPs) are minor components bound with starch granule, whose variation could impact starch properties. This study investigated, for the first time, the variation of SGPs in the cultivated naked barley from Qinghai-Tibet Plateau in China. The relation-ship between SGPs and starch content was preliminarily dealt with. Ten major SGPs and 16 types of patterns were present in the 66 cultivated naked varieties, indicating that the SGPs in cultivated naked barley from Qinghai-Tibet Plateau in China are polymorphic. The SGPs of naked barley in Tibet and Sichuan were greatly different and the SGP patterns were specific to sampling regions. Significance test analysis demonstrated that the SGPs described in this study, except for SGP1, could be related with the variation of starch content in the different naked barleys.

  5. [SGP polymorphism in cultivated naked barley from Qinghai-Tibet plateau in China and the relationship between SGPs and starch content].

    Science.gov (United States)

    Pan, Zhi-Fen; Zhou, Yi-Xing; Zhao, Tao; Deng, Guang-Bing; Zhai, Xu-Guang; Wu, Fang; Yu, Mao-Qun

    2007-05-01

    Starch granule proteins (SGPs) are minor components bound with starch granule, which mutation may be related to starch properties. This study investigated the variation of SGPs in cultivated naked barley from Qinghai-Tibet Plateau in China for the first time, and the relationship between SGPs and starch content was preliminarily done. Ten major SGPs and 16 types of patterns were present in 66 cultivated naked varieties, indicating SGPs in cultivated naked barley from Qinghai-Tibet Plateau in China are polymorphic. SGPs in Tibet and Sichuan naked barley were greatly different and SGPs were specific to origin of site. Significance test analysis demonstrates SGPs described in this study except for SGP1 may be related with the variation of starch content in different naked barley.

  6. Magnetic purification of curcumin from Curcuma longa rhizome by novel naked maghemite nanoparticles.

    Science.gov (United States)

    Magro, Massimiliano; Campos, Rene; Baratella, Davide; Ferreira, Maria Izabela; Bonaiuto, Emanuela; Corraducci, Vittorino; Uliana, Maíra Rodrigues; Lima, Giuseppina Pace Pereira; Santagata, Silvia; Sambo, Paolo; Vianello, Fabio

    2015-01-28

    Naked maghemite nanoparticles, namely, surface active maghemite nanoparticles (SAMNs), characterized by a diameter of about 10 nm, possessing peculiar colloidal stability, surface chemistry, and superparamagnetism, present fundamental requisites for the development of effective magnetic purification processes for biomolecules in complex matrices. Polyphenolic molecules presenting functionalities with different proclivities toward iron chelation were studied as probes for testing SAMN suitability for magnetic purification. Thus, the binding efficiency and reversibility on SAMNs of phenolic compounds of interest in the pharmaceutical and food industries, namely, catechin, tyrosine, hydroxytyrosine, ferulic acid, coumaric acid, rosmarinic acid, naringenin, curcumin, and cyanidin-3-glucoside, were evaluated. Curcumin emerged as an elective compound, suitable for magnetic purification by SAMNs from complex matrices. A combination of curcumin, demethoxycurcumin, and bis-demethoxycurcumin was recovered by a single magnetic purification step from extracts of Curcuma longa rhizomes, with a purity >98% and a purification yield of 45%, curcumin being >80% of the total purified curcuminoids.

  7. Development of a Textile Nanocomposite as Naked Eye Indicator of the Exposition to Strong Acids

    Directory of Open Access Journals (Sweden)

    Isabel Pallás

    2017-09-01

    Full Text Available Chemical burns, mainly produced by acids, are a topic of concern. A new sensing material for the detection of strong acids able to be incorporated into textiles has been developed. The material is prepared by the covalent attachment of 2,2′,4,4′,4″-pentamethoxy triphenyl methanol to a mesoporous material which further is included in a nitro resin to obtain a colourless composite. The response of this composite to diverse acid solutions was tested showing the appearance of an intense purple colour (with a colour difference higher than 160 that can be monitored by the naked eye or could be easily digitised to feed an instrumental sensor. Reversibility and resistance to washing cycles were studied with positive results. Finally, the response of the sensing composite to acid vapours was assayed, observing a colour change similar to that found in solution.

  8. Does the particle creation mechanism favour the formation of a black hole or a naked singularity?

    CERN Document Server

    Bhattacharjee, Sudipto; Chakraborty, Subenoy

    2016-01-01

    The paper deals with collapse dynamics of a spherically symmetric massive star in the framework of non-equilibrium themodynamic prescription through particle creation mechanism. The matter content in the star is in the form of perfect uid with barotropic equation of state and the dissipative phenomena due to non-equilibrium thermodynamics is in the form of bulk viscosity. For simplicity, the thermodynamic system is chosen to be adiabatic (i.e., isentropic) so that the e?ective bulk viscous pressure is linearly related to the particle creation rate. As a result, the evolution of the collapsing star also depends on the particle creation rate. By proper choice of creation rate as a function of the Hubble parameter, it is found that the end state of the collapse may be either a black hole or a naked singularity.

  9. Apple scar skin viroid naked RNA is actively transmitted by the whitefly Trialeurodes vaporariorum.

    Science.gov (United States)

    Walia, Yashika; Dhir, Sunny; Zaidi, Aijaz Asghar; Hallan, Vipin

    2015-01-01

    Nucleic acid transfer between plants is a phenomenon which is likely to occur in many ways in nature. We report here the active transmission of Apple scar skin viroid (ASSVd) naked ssRNA species by the whitefly Trialeurodes vaporariorum (Tv). Not only the viroid RNA, its DNA form was also identified from the insect. The viroid transfer efficiency was enhanced with the help of Cucumis sativus Phloem protein 2 (CsPP2), a plant protein known to translocate viroid RNAs. This PP2/ASSVd complex is stably present in the viroid infected cucumber plants, as was identified with the help of immunological reaction. As viroid-like secondary structures are found in some plant RNAs, and PP2 is known to bind and translocate several RNAs, the results have huge implications in transfer of these RNA species between plants visited by the whitefly.

  10. Naked eye observations for morning twilight at different sites in Egypt

    Directory of Open Access Journals (Sweden)

    A.H. Hassan

    2014-06-01

    Full Text Available Twilight observations were carried out in the period between 1984 and 1987 in different seasons at different sites of Egypt (Baharia, Matrouh, Kottamia and Aswan through a cooperation project between Dar El-Iftaa’ and the Egyptian Academy of Scientific Research and Technology. Naked eye observations of the first light of the dawn were done in parallel to the photoelectric measurements of the twilight phenomena. The depression of the sun below the horizon corresponding to the first light was calculated from the time of observations. Our estimates show that the normal eye can just discriminate the dawn (the first white light thread at a depression of 14.7° with a maximum value of 15.08° and a minimum value of 12.01°. This result agrees with result obtained by our previous photoelectric measurements.

  11. Naked-eye detection of biologically important anions by a new chromogenic azo-azomethine sensor.

    Science.gov (United States)

    Rezaeian, Khatereh; Khanmohammadi, Hamid

    2014-12-10

    A new chromogenic azo-azomethine sensor, containing active phenolic sites, has been designed and synthesized via condensation reaction of N,N,N',N'-tetrakis(2-aminoethyl)-2,2-dimethyl propane-1,3-diamine with 1-(3-formyl-4-hydroxyphenylazo)-4-nitrobenzene. The anion recognition ability of the synthesized receptor was evaluated using UV-Vis spectroscopy and (1)H NMR technique. The anion recognition studies exhibited that the receptor acts as a sensor for biologically important anions such as F(-), AcO(-) and H2PO4(-) over other anions. The binding stoichiometry between sensor and anions was found to be 1:2. (1)H NMR experiment revealed that sensor recognizes anions via H-bonds and subsequent deprotonation to elicit a vivid color change. Interestingly, the sensory system not only let for the naked eye detection without any spectroscopic instrumentation but also helped to discriminate between anions.

  12. Writing the Naked Body: Sex and Nudity in Nuala Ní Chonchuír’s Nude

    Directory of Open Access Journals (Sweden)

    Máximo Aláez-Corral

    2016-03-01

    Full Text Available This essay looks into the deconstruction of the male gaze and the objectification process around the concepts of nudity and nakedness. Starting from the review of some of Martha Nussbaum’s, John Berger’s, or Laura Mulvey’s main ideas on the topic of the gaze and objectification, a practical analysis of some of the short stories contained in Nuala Ní Chonchúir’s Nude (2009 is provided. By so doing, I intend to examine the ways in which conventional objectification phenomena can be turned upside down, challenged or parodied in order to reveal cultural discriminatory undercurrents and offer a new non-objectifying visual and written representation of the naked body.

  13. Optimization of Naked DNA Delivery for Interferon Subtype Immunotherapy in Cytomegalovirus Infection

    Directory of Open Access Journals (Sweden)

    Bartlett Emmalene J.

    2003-01-01

    Full Text Available Type I interferon (IFN gene therapy modulates the immune response leading to inflammatory heart disease following cytomegalovirus (CMV infection in a murine model of post-viral myocarditis. Efficacy of different immunisation protocols for the IFN constructs was influenced by the dose of DNA, subtype choice, combination use, pre-medication, and timing of DNA administration. Optimal efficacy was found with bupivacaine treatment prior to DNA inoculation of 200mg IFN DNA 14 days prior to virus challenge. Maximal antiviral and antimyocarditic effects were achieved with this vaccination schedule. Furthermore, inoculation of synergistic IFN subtypes demonstrated enhanced efficacy when delivered either alone or with CMV gB DNA vaccination in the CMV model. Thus naked DNA delivery of IFN provides an avenue of immunotherapy for regulating herpesvirus-induced diseases.

  14. A genetic linkage map of hexaploid naked oat constructed with SSR markers

    Institute of Scientific and Technical Information of China (English)

    Gaoyuan; Song; Pengjie; Huo; Bin; Wu; Zongwen; Zhang

    2015-01-01

    Naked oat is a unique health food crop in China. Using 202 F2 individuals derived from a hybrid between the variety 578 and the landrace Sanfensan, we constructed a genetic linkage map consisting of 22 linkage groups covering 2070.50 c M and including 208 simple sequence repeat(SSR) markers. The minimum distance between adjacent markers was0.01 c M and the average was 9.95 c M. Each linkage group contained 2–22 markers. The largest linkage group covered 174.40 c M and the shortest one covered 36.80 c M, with an average of 94.11 c M. Thirty-six markers(17.3%) showing distorted segregation were distributed across linkage groups LG5 to LG22. This map complements published oat genetic maps and is applicable for quantitative trait locus analysis, gene cloning and molecular marker-assisted selection.

  15. "Naked-eye" colorimetric and "turn-on" fluorometric chemosensors for reversible Hg2+ detection.

    Science.gov (United States)

    Wanichacheva, Nantanit; Praikaew, Panida; Suwanich, Thanapat; Sukrat, Kanjarat

    2014-01-24

    Two new Hg(2+)-colorimetric and fluorescent sensors based on 2-[3-(2-aminoethylsulfanyl) propylsulfanyl]ethanamine covalently bound to one and two units of rhodamine-6G moieties, 1 and 2, were synthesised, and their sensing behaviors toward metal ions were investigated by UV/Vis and fluorescence spectroscopy. Upon the addition of Hg(2+), the sensors exhibited highly sensitive "turn-on" fluorescence enhancement as well as a color change from colorless to pink, which was readily noticeable for naked eye detection. Especially, 1 exhibited the reversible behavior and revealed a very high selectivity in the presence of competitive ions, particularly Cu(2+), Ag(+), Pb(2+), Ca(2+), Cd(2+), Co(2+), Fe(2+), Mn(2+), Na(+), Ni(2+), K(+), Ba(2+), Li(+) and Zn(2+), with a low detection limit of 1.7 ppb toward Hg(2+).

  16. Naked eye determination of the dawn for Sinai and Assiut of Egypt

    Directory of Open Access Journals (Sweden)

    A.H. Hassan

    2016-06-01

    Full Text Available Twilight observations were carried out by naked eye in the period (2010–2012 for north Sinai (Lat. 31°4′N, Long. 32°52′E where the background is desert, and for Assiut (Lat. 27°10′N, Long. 31°10′E in the period (2012–2014 where the background is agricultural land. The purpose of these observations is to calculate the depression of the sun below the horizon at which the normal eye can discriminate the dawn (morning white thread for two sites. The results indicated that this discrimination takes place at vertical sun depression angles, Do = 14.61° and 13.665° at Sinai and Assiut respectively.

  17. Review of Naked Statistics: Stripping the Dread from Data by Charles Wheelan

    Directory of Open Access Journals (Sweden)

    Michael T. Catalano

    2015-01-01

    Full Text Available Wheelan, Charles. Naked Statistics: Stripping the Dread from Data (New York, NY, W. W. Norton & Company, 2014. 282 pp. ISBN 978-0-393-07195-5 In his review of What Numbers Say and The Numbers Game, Rob Root (Numeracy 3(1: 9 writes “Popular books on quantitative literacy need to be easy to read, reasonably comprehensive in scope, and include examples that are thought-provoking and memorable.” Wheelan’s book certainly meets this description, and should be of interest to both the general public and those with a professional interest in numeracy. A moderately diligent learner can get a decent understanding of basic statistics from the book. Teachers of statistics and quantitative literacy will find a wealth of well-related examples and stories to use in their classes.

  18. Efficiency of the Keplerian accretion in the Braneworld Kerr--Newman Spacetimes and mining instability of some naked singularity spacetimes

    CERN Document Server

    Blaschke, Martin

    2016-01-01

    We show that the~braneworld rotating Kerr--Newman black hole and naked singularity spacetimes with both positive and negative braneworld tidal charge parameter can be separated into fourteen classes according to properties of circular geodesics governing the~Keplerian accretion. We determine efficiency of the~Keplerian accretion disks for all braneworld Kerr--Newman spacetimes. We demonstrate occurrence of an~infinitely deep gravitational potential in Kerr--Newman naked singularity spacetimes having the~braneworld dimensionless tidal charge $b \\in (1/4,1)$ and the~dimensionless spin $a \\in (2\\sqrt{b}-\\sqrt{b(4b-1)},2\\sqrt{b}+\\sqrt{b(4b-1)})$, implying unbound efficiency of the~Keplerian accretion and possibility to extract the~whole naked singularity mass. Therefore, we call them braneworld "mining-unstable" Kerr--Newman naked singularity spacetimes. Fundamental restriction on the~relevance of the~extraordinary but fully classical phenomenon of the~mining instability is given by validity of the~assumption of ...

  19. Naked (C5Me5)(2)M cations (M = Sc, Ti, and V) and their fluoroarene complexes

    NARCIS (Netherlands)

    Bouwkamp, MW; Budzelaar, PHM; Gercama, J; Morales, ID; de Wolf, J; Meetsma, A; Troyanov, SI; Teuben, JH; Hessen, B; Budzelaar, Peter H.M.; Hierro Morales, Isabel Del; Troyanov, Sergei I.

    2005-01-01

    The ionic metallocene complexes [Cp*M-2][BPh4] (CP* = C5Me5) of the trivalent 3d metals Sc, Ti, and V were synthesized and structurally characterized. For M Sc, the anion interacts weakly with the metal center through one of the phenyl groups, but for M = Ti and V, the cations are naked. They each c

  20. The effect of phytohormones on the dynamics of protein biosynthesis and enzyme activity in linted and naked cotton seed

    Science.gov (United States)

    We determined the effect of exogenous indole-3-acetic acid, a-naphthylene-3-acetic acid and gibberellic acid (GA3) on the enzymatic activity of glucansynthase, peroxidase and cellulase in ovule development of naked L-70 and linted AN-Bayaut-2 cotton (Gossypium hirsutum L.) seeds. We isolated a prote...

  1. Molecular analysis of cultivated naked barley (Hordeum vulgare L.) from Qinghai-Tibet Plateau in China using SSR markers

    Institute of Scientific and Technical Information of China (English)

    Zhifen PAN; Guangbing DENG; Xuguang ZHAI; Hai LONG; Yawei TANG; Xiaolin QIANG; Maoqun YU

    2008-01-01

    Naked barley is widely planted in Qinghai-Tibet Plateau in China and is essential for the daily life of Tibetans in those regions. In this study, the genetic diversity of 64 cultivated naked barley accessions from Qinghai-Tibet Plateau in China was analyzed using 30 mapped SSRs linked with the important traits of barley improvement. A total of 132 alleles were identified at 22 polymorphic SSR loci, with the number of each locus ranging from 2 to 15, the polymorphism information con-tent (PIC) values ranging from 0.16 to 0.91, and with an average of 0.65. Of the selected SSRs, 13 SSR markers with high PIC value were highly efficient for the genetic analysis of Chinese barley. The accessions were divided into five main groups by cluster analysis and could be differentiated from each other. The genetic diversity in the Tibet accessions was slightly higher than in the Sichuan accessions. It is found that there were specific genes linked with the collecting sites. These results indi-cate the cultivated naked barley from Qinghai-Tibet Plateau in China are highly polymorphic and could be considered as an important resource bank for cultivated naked barley breeding in the future.

  2. Oxygen-Binding Characteristics of Hemoglobins from Hypoxia and Hypercapnia Tolerant African Mole Rats

    DEFF Research Database (Denmark)

    Weber, Roy E.; Jarvis, Jennifer U. M; Fago, Angela

    2016-01-01

    to cancer, and the capability of tolerating more extreme levels of hypoxia (O2 tensions lower than on Mount Everest) and hypercapnia (10% CO2 concentrations) than other mammals. With the view of identifying possible cellular and molecular mechanisms that mitigate the constraints on the exchange and internal...

  3. Transcriptome, genetic editing, and microRNA divergence substantiate sympatric speciation of blind mole rat, Spalax

    National Research Council Canada - National Science Library

    Li, Kexin; Wang, Liuyang; Knisbacher, Binyamin A; Xu, Qinqin; Levanon, Erez Y; Wang, Huihua; Frenkel-Morgenstern, Milana; Tagore, Satabdi; Fang, Xiaodong; Bazak, Lily; Buchumenski, Ilana; Zhao, Yang; Lövy, Matěj; Li, Xiangfeng; Han, Lijuan; Frenkel, Zeev; Beiles, Avigdor; Cao, Yi Bin; Wang, Zhen Long; Nevo, Eviatar

    2016-01-01

    ... on mitochondrial and whole-genome nuclear DNA. Here, we present new evidence, including transcriptome, DNA editing, microRNA, and codon usage, substantiating earlier evidence for adaptive divergence in the abutting chalk and basalt populations...

  4. The reproductive biology of the giant Zambian mole-rat, Cryptomys ...

    African Journals Online (AJOL)

    1994-09-29

    Sep 29, 1994 ... pare growth patterns in the particular Cryptomys species. (Table I). ... 2 thrusts per second) reaching a peak. prior to ejaculation. ... tation period of approximately 111 days (Figure 1). In a sec- ... rest of the time they sleep.

  5. 3d soil structure within the mound of mole rats (Spalax microphthalmus

    Directory of Open Access Journals (Sweden)

    T. М. Konovalova

    2011-10-01

    Full Text Available The pedoturbation activity of the soil-dwelling mammals results in the complicated spatial soil structure with combined mechanically stable dense soil and the soil mass of low density. Such organization of the soil body has useful combination of the soil properties. The prolonged effect of the mammals’ pedoturbation activity is positive for different constituents of an ecosystem: soil animals, microorganisms and plants.

  6. Sympatric speciation revealed by genome-wide divergence in the blind mole rat Spalax.

    Science.gov (United States)

    Li, Kexin; Hong, Wei; Jiao, Hengwu; Wang, Guo-Dong; Rodriguez, Karl A; Buffenstein, Rochelle; Zhao, Yang; Nevo, Eviatar; Zhao, Huabin

    2015-09-22

    Sympatric speciation (SS), i.e., speciation within a freely breeding population or in contiguous populations, was first proposed by Darwin [Darwin C (1859) On the Origins of Species by Means of Natural Selection] and is still controversial despite theoretical support [Gavrilets S (2004) Fitness Landscapes and the Origin of Species (MPB-41)] and mounting empirical evidence. Speciation of subterranean mammals generally, including the genus Spalax, was considered hitherto allopatric, whereby new species arise primarily through geographic isolation. Here we show in Spalax a case of genome-wide divergence analysis in mammals, demonstrating that SS in continuous populations, with gene flow, encompasses multiple widespread genomic adaptive complexes, associated with the sharply divergent ecologies. The two abutting soil populations of S. galili in northern Israel habituate the ancestral Senonian chalk population and abutting derivative Plio-Pleistocene basalt population. Population divergence originated ∼0.2-0.4 Mya based on both nuclear and mitochondrial genome analyses. Population structure analysis displayed two distinctly divergent clusters of chalk and basalt populations. Natural selection has acted on 300+ genes across the genome, diverging Spalax chalk and basalt soil populations. Gene ontology enrichment analysis highlights strong but differential soil population adaptive complexes: in basalt, sensory perception, musculature, metabolism, and energetics, and in chalk, nutrition and neurogenetics are outstanding. Population differentiation of chemoreceptor genes suggests intersoil population's mate and habitat choice substantiating SS. Importantly, distinctions in protein degradation may also contribute to SS. Natural selection and natural genetic engineering [Shapiro JA (2011) Evolution: A View From the 21st Century] overrule gene flow, evolving divergent ecological adaptive complexes. Sharp ecological divergences abound in nature; therefore, SS appears to be an important mode of speciation as first envisaged by Darwin [Darwin C (1859) On the Origins of Species by Means of Natural Selection].

  7. Influence of spatial environment on maze learning in an African mole-rat

    CSIR Research Space (South Africa)

    Du Toit, L

    2012-05-01

    Full Text Available of Animal, Plant and Environmental Sciences, University of the Witwatersrand, Private Bag 3, Johannesburg, Wits 2050, South Africa e-mail: martin.whiting@mq.edu.au Present Address: L. du Toit, Obesity Research and Management, University of Alberta...

  8. Fluoride-Triggered Ring-Opening of Photochromic Diarylpyrans into Merocyanine Dyes: Naked-Eye Sensing in Subppm Levels.

    Science.gov (United States)

    Mukhopadhyay, Arindam; Maka, Vijay Kumar; Moorthy, Jarugu Narasimha

    2016-09-02

    The fluoride-mediated desilylation reaction has been exploited, for the first time, to trigger ring-opening of photochromic diarylbenzo-/naphthopyrans into highly colored anionic merocyanine dyes with high molar absorptivities to permit naked-eye sensing. The absorption spectral shifts, i.e., differences in the absorption maxima of colorless and colored forms, observed for a rationally designed set of silyloxy-substituted diarylpyrans subsequent to fluoride-induced ring opening are remarkably high (330-480 nm), and are unknown for any colorimetric probe. In particular, the disilyloxy-substituted diphenylnaphthopyran and its analog, in which the diphenyl groups are fused in the form of fluorene, allows "naked-eye" detection of fluoride in subppm levels (photochromic benzo-/naphthopyrans is heretofore unprecedented.

  9. Existence of naked singularities in the Brans-Dicke theory of gravitation. An analytical and numerical study

    Energy Technology Data Exchange (ETDEWEB)

    Bedjaoui, Nabil [LMFA and INSSET, Universite de Picardie Jules Verne, 48 rue Raspail, 02100 St Quentin (France); LeFloch, Philippe G [Laboratoire Jacques-Louis Lions and Centre National de la Recherche Scientifique, Universite Pierre et Marie Curie (Paris 6), 4 Place Jussieu, 75252 Paris (France); Martin-Garcia, Jose M [Institut d' Astrophysique de Paris and Centre National de la Recherche Scientifique, Universite Pierre et Marie Curie, 98bis Boulevard Arago, 75014 Paris (France); Novak, Jerome, E-mail: Bedjaoui@u-picardie.f, E-mail: pgLeFloch@gmail.co, E-mail: Garcia@iap.f, E-mail: Jerome.Novak@obspm.f [Laboratoire Univers et Theories, Observatoire de Paris and Centre National de la Recherche Scientifique, Universite Paris Diderot, 5 Place Jules Janssen, 92190 Meudon (France)

    2010-12-21

    Within the framework of the scalar-tensor models of gravitation and by relying on analytical and numerical techniques, we establish the existence of a class of spherically symmetric spacetimes containing a naked singularity. Our result relies on and extends a work by Christodoulou on the existence of naked singularities for the Einstein-scalar field equations. We establish that a key parameter in Christodoulou's construction couples to the Brans-Dicke field and becomes a dynamical variable, which enlarges and modifies the phase space of solutions. We recover analytically many properties first identified by Christodoulou, in particular the loss of regularity (especially at the center), and then investigate numerically the properties of these spacetimes.

  10. Particle Collision Near 1 + 1-Dimensional Horava-Lifshitz Black Hole and Naked Singularity

    Directory of Open Access Journals (Sweden)

    M. Halilsoy

    2017-01-01

    Full Text Available The unbounded center-of-mass (CM energy of oppositely moving colliding particles near horizon emerges also in 1+1-dimensional Horava-Lifshitz gravity. This theory has imprints of renormalizable quantum gravity characteristics in accordance with the method of simple power counting. Surprisingly the result obtained is not valid for a 1-dimensional Compton-like process between an outgoing photon and an infalling massless/massive particle. It is possible to achieve unbounded CM energy due to collision between infalling photons and particles. The source of outgoing particles may be attributed to an explosive process just outside the horizon for a black hole and the naturally repulsive character for the case of a naked singularity. It is found that absence of angular momenta in 1+1-dimension does not yield unbounded energy for collisions in the vicinity of naked singularities.

  11. Muscle damage after delivery of naked plasmid DNA into skeletal muscles is batch dependent.

    Science.gov (United States)

    Wooddell, Christine I; Subbotin, Vladimir M; Sebestyén, Magdolna G; Griffin, Jacob B; Zhang, Guofeng; Schleef, Martin; Braun, Serge; Huss, Thierry; Wolff, Jon A

    2011-02-01

    Various plasmids were delivered into rodent limb muscles by hydrodynamic limb vein (HLV) injection of naked plasmid DNA (pDNA). Some of the pDNA preparations caused significant muscle necrosis and associated muscle regeneration 3 to 4 days after the injection whereas others caused no muscle damage. Occurrence of muscle damage was independent of plasmid sequence, size, and encoded genes. It was batch dependent and correlated with the quantity of bacterial genomic DNA (gDNA) that copurified with the pDNA. To determine whether such an effect was due to bacterial DNA or simply to fragmented DNA, mice were treated by HLV injection with sheared bacterial or murine gDNA. As little as 20 μg of the large fragments of bacterial gDNA caused muscle damage that morphologically resembled damage caused by the toxic pDNA preparations, whereas murine gDNA caused no damage even at a 10-fold higher dose. Toxicity from the bacterial gDNA was not due to endotoxin and was eliminated by DNase digestion. We conclude that pDNA itself does not cause muscle damage and that purification methods for the preparation of therapeutic pDNA should be optimized for removal of bacterial gDNA.

  12. Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

    Science.gov (United States)

    Hashmat, Uzma; Yousaf, Muhammad; Lal, Bhajan; Ullah, Shafiq; Holder, Alvin A.; Badshah, Amin

    2016-01-01

    DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1, UA6 and UA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 × 103, 2.4 × 103 and 0.2 × 103 M−1, for UA1, UA6 and UA7, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the λmax value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ng µl−1 for UA1, UA6 and UA7, respectively. PMID:28018613

  13. How do mineral fertilization and plant growth regulators affect yield and morphology of naked oat?

    Directory of Open Access Journals (Sweden)

    Robert Witkowicz

    2010-09-01

    Full Text Available Oat (Avena sativa var. nuda is of an increasing interest in many parts of the world. This is why plant breeders have developed forms that are morphologically different from the current ones, such as naked, dwarf or with an increased 1000-grain-weight. In three experiments conducted at two sites, the influence of phosphorus (P and potassium (K fertilizers, spray application of urea and spray application of plant growth regulators (PGRs Promalin (gibberellins + cytokinin and Moddus (cimectacarps on the yield and morphological traits of different oat forms were studied. At a better site, only genotype statistically influenced oat grain yield. At a poorer site, apart from genotype there were statistically significant responses to P and K fertilizers and to the application of Moddus (especially in the experiment with a dwarf cultivar. The internode and panicle length were modified mostly by cimectacarps, which shortened specific internodes, but not the panicle. The PGR Promalin had no significant effect on oat stem morphology.

  14. A Rapid In Situ Colorimetric Assay for Cobalt Detection by the Naked Eye

    Directory of Open Access Journals (Sweden)

    Sung-Min Kang

    2016-05-01

    Full Text Available A simple, rapid, and convenient colorimetric chemosensor of a specific target toward the end user is still required for on-site detection and real-time monitoring applications. In this study, we developed a rapid in situ colorimetric assay for cobalt detection using the naked eye. Interestingly, a yellow to light orange visual color transition was observed within 3 s when a Chrysoidine G (CG chemosensor was exposed to cobalt. Surprisingly, the CG chemosensor had great selectivity toward cobalt without any interference of other metal ions. Under optimized conditions, a lower detection limit of 0.1 ppm via a spectrophotometer and a visual detection limit of 2 ppm with a linear range from 0.4 to 1 ppm (R2 = 0.97 were determined. Moreover, the CG chemosensor is reversible and maintains its functionality after treatment with chelating agents. In conclusion, we show the superior capabilities of the CG chemosensor, which has the potential to provide extremely facile handling, high sensitivity, and a fast response time for applications of on-site detection to real-time cobalt monitoring for the general public.

  15. Electrochromic Asymmetric Supercapacitor Windows Enable Direct Determination of Energy Status by the Naked Eye.

    Science.gov (United States)

    Zhong, Ying; Chai, Zhisheng; Liang, Zhimin; Sun, Peng; Xie, Weiguang; Zhao, Chuanxi; Mai, Wenjie

    2017-09-19

    Because of the popularity of smart electronics, multifunctional energy storage devices, especially electrochromic supercapacitors (SCs), have attracted tremendous research interest. Herein, a solid-state electrochromic asymmetric SC (ASC) window is designed and fabricated by introducing WO3 and polyaniline as the negative and positive electrodes, respectively. The two complementary materials contribute to the outstanding electrochemical and electrochromic performances of the fabricated device. With an operating voltage window of 1.4 V and an areal capacitance of 28.3 mF cm(-2), the electrochromic devices show a high energy density of 7.7 × 10(-3) mW h cm(-2). Meanwhile, they exhibit an obvious and reversible color transition between light green (uncharged state) and dark blue (charged state), with an optical transmittance change between 55 and 12% at a wavelength of 633 nm. Hence, the energy storage level of the ASC is directly related to its color and can be determined by the naked eye, which means it can be incorporated with other energy cells to visual display their energy status. Particularly, a self-powered and color-indicated system is achieved by combining the smart windows with commercial solar cell panels. We believe that the novel electrochromic ASC windows will have great potential application for both smart electronics and smart buildings.

  16. Observations of the Naked-Eye GRB 080319B: Implications of Nature's Brightest Explosion

    CERN Document Server

    Bloom, J S; Li, W; Butler, N R; Miller, A A; Kocevski, D; Kann, D A; Foley, R J; Chen, H -W; Filippenko, A V; Starr, D L; Macomber, B; Prochaska, J X; Chornock, R; Poznanski, D; Klose, S

    2008-01-01

    The first gamma-ray burst (GRB) confirmed to be bright enough to be seen with the naked eye, GRB 080319B, allowed for exquisite follow-up across the electromagnetic spectrum. We present our detailed optical and infrared observations of the afterglow, consisting of over 5000 images starting 122 s after the GRB trigger, in concert with our own analysis of the Swift UVOT, BAT, and XRT data. The event is extreme not only in observed properties but intrinsically: it was the most luminous ever recorded at optical wavelengths and had an exceedingly high isotropic-equivalent energy release in gamma-rays. At early times, the afterglow evolution is broadly consistent with being reverse-shock dominated, then is subsumed by a forward shock at around 1000 s. Analysis of the forward shock suggests that the remarkable energetics of this burst may be owed largely to extreme collimation. The spectral energy distribution, spanning from ultraviolet through near-infrared, shows no evidence for a significant amount of dust extinc...

  17. GRB 080503: Implications of a Naked Short Gamma-Ray Burst Dominated by Extended Emission

    CERN Document Server

    Perley, D A; Granot, J; Butler, N R; Sakamoto, T; Ramirez-Ruiz, E; Levan, A J; Bloom, J S; Miller, A A; Bunker, A; Chen, H -W; Filippenko, A V; Gehrels, N; Glazebrook, K; Hall, P; Hurley, K C; Kocevski, D; Li, W; López, S; Norris, J; Piro, A L; Poznanski, D; Prochaska, J X; Quataert, E; Tanvir, N

    2008-01-01

    We report on observations of GRB 080503, a short gamma-ray burst with very bright extended emission (about 30 times the gamma-ray fluence of the initial spike) in conjunction with a thorough comparison to other short Swift events. In spite of the prompt-emission brightness, however, the optical counterpart is extraordinarily faint, never exceeding 25 mag in deep observations starting at ~1 hr after the BAT trigger. The optical brightness peaks at ~1 day and then falls sharply in a manner similar to the predictions of Li & Paczynski (1998) for supernova-like emission following compact-binary mergers. However, a shallow spectral index and similar evolution in X-rays inferred from Chandra observations are more consistent with an afterglow interpretation. The extreme faintness of this probable afterglow relative to the bright gamma-ray emission argues for a very low-density medium surrounding the burst (a "naked" GRB), consistent with the lack of a coincident host galaxy down to 28.5 mag in deep HST imaging. ...

  18. Hydrogen production by the naked active site of the di-iron hydrogenases in water.

    Science.gov (United States)

    Zipoli, Federico; Car, Roberto; Cohen, Morrel H; Selloni, Annabella

    2009-10-01

    We explored the reactivity of the active center of the [FeFe]-hydrogenases detached from the enzyme and immersed in acidified water by first-principles Car-Parrinello molecular-dynamics simulations. We focused on the identification of the structures that are stable and metastable in acidified water and on their activity for hydrogen production. Our calculations revealed that the naked active center could be an efficient catalyst provided that electrons are transferred to the cluster. We found that both bridging and terminal isomers are present at equilibrium and that the bridging configuration is essential for efficient hydrogen production. The formation of the hydrogen molecule occurs via sequential protonations of the distal iron and of the N-atom of the S-CH(2)-NH-CH(2)-S chelating group. H(2) desorption does not involve a significant energy barrier, making the process very efficient at room temperature. We established that the bottleneck in the reaction is the direct proton transfer from water to the vacant site of the distal iron. Moreover, we found that even if the terminal isomer is present at the equilibrium, its strong local hydrophobicity prevents poisoning of the cluster.

  19. The initial value problem for linearized gravitational perturbations of the Schwarzschild naked singularity

    Science.gov (United States)

    Dotti, Gustavo; Gleiser, Reinaldo J.

    2009-11-01

    The coupled equations for the scalar modes of the linearized Einstein equations around Schwarzschild's spacetime were reduced by Zerilli to a (1+1) wave equation \\partial ^2 \\Psi _z / \\partial t^2 + {\\cal H} \\Psi _z =0 , where {\\cal H} = -\\partial ^2 / \\partial x^2 + V(x) is the Zerilli 'Hamiltonian' and x is the tortoise radial coordinate. From its definition, for smooth metric perturbations the field Ψz is singular at rs = -6M/(ell - 1)(ell +2), with ell being the mode harmonic number. The equation Ψz obeys is also singular, since V has a second-order pole at rs. This is irrelevant to the black hole exterior stability problem, where r > 2M > 0, and rs 0, and the singularity appears in the relevant range of r (0 value of M. The relation of \\hat{\\Psi} to Ψz is provided by an intertwiner operator. The spatial pieces of the (1 + 1) wave equations that \\hat{\\Psi} and Ψz obey are related as a supersymmetric pair of quantum Hamiltonians {\\cal H} and \\hat{\\cal H} . For Mproof of the linear instability of the Schwarzschild naked singularity, by showing that a previously found unstable mode belongs to a complete basis of \\hat{\\cal H} in {\\cal D} , and thus is excitable by generic initial data. This is further illustrated by numerically solving the linearized equations for suitably chosen initial data.

  20. Targeting of cytosolic mRNA to mitochondria: naked RNA can bind to the mitochondrial surface.

    Science.gov (United States)

    Michaud, Morgane; Maréchal-Drouard, Laurence; Duchêne, Anne-Marie

    2014-05-01

    Mitochondria contain hundreds of proteins but only a few are encoded by the mitochondrial genome. The other proteins are nuclear-encoded and imported into mitochondria. These proteins can be translated on free cytosolic polysomes, then targeted and imported into mitochondria. Nonetheless, numerous cytosolic mRNAs encoding mitochondrial proteins are detected at the surface of mitochondria in yeast, plants and animals. The localization of mRNAs to the vicinity of mitochondria would be a way for mitochondrial protein sorting. The mechanisms responsible for mRNA targeting to mitochondria are not clearly identified. Sequences within the mRNA molecules (cis-elements), as well as a few trans-acting factors, have been shown to be essential for targeting of some mRNAs. In order to identify receptors involved in mRNA docking to the mitochondrial surface, we have developed an in vitro mRNA binding assay with isolated plant mitochondria. We show that naked mRNAs are able to bind to isolated mitochondria, and our results strongly suggest that mRNA docking to the plant mitochondrial outer membrane requires at least one component of TOM complex.

  1. Strong Curvature Naked Singularities in the Gravitational Collapse of a Homogeneous Weyssenhoff Fluid

    CERN Document Server

    Ziaie, Amir Hadi; Ranjbar, Arash; Sepangi, Hamid Reza

    2014-01-01

    We consider the gravitational collapse of matter, whose content is a Weyssenhoff fluid, in the presence of a negative cosmological constant. Such fluid conveys effects that can be associated to fermionic spin-angular momentum features suitably averaged. Our purpose in this paper is precisely to investigate how those effects may influence the final outcome of the mentioned collapse. For a specific spacetime setup (namely the Tolman-Bondi marginally bound case), we obtain a family of exact solutions to the field equations, which exhibits a spacetime singularity. We further find that, for a specific subset of those solutions, the formation of trapped surfaces can be prevented. Whether a naked singularity emerges or not, this is shown to depend on parameters of the Weyssenhoff fluid, which are related to an averaged spin density as well as to an axial current. The nature of the central singularity is examined and it is shown that null trajectories can emerge from the singularity, exposing it at least to neighbori...

  2. Self-similar cosmological solutions with dark energy II: black holes, naked singularities and wormholes

    CERN Document Server

    Maeda, Hideki; Carr, B J

    2007-01-01

    We use a combination of numerical and analytical methods, exploiting the equations derived in an accompanying paper, to classify all spherically symmetric self-similar solutions which are asymptotically Friedmann at large distances and contain a perfect fluid with equation of state $p=(\\gamma -1)\\mu$ with $0<\\gamma<2/3$. The expansion of the Friedmann universe is accelerated in this case. We find a one-parameter family of self-similar solutions representing a black hole embedded in a Friedmann background. This suggests that, in contrast to the positive pressure case, black holes in a universe with dark energy can grow as fast as the Hubble horizon if they are not too large. There are also self-similar solutions which contain a central naked singularity with negative mass. We also find various kinds of self-similar wormhole solutions; these represent a Friedmann universe connected to either another Friedmann universe or some other cosmological model. These wormholes are generally traversable, where we de...

  3. Gamma-Ray Burst at the extreme: "the naked-eye burst" GRB 080319B

    CERN Document Server

    Wozniak, P R; Panaitescu, A D; Wren, J A; Davis, H R; White, R R

    2008-01-01

    On 19 March 2008, the northern sky was the stage of a spectacular optical transient that for a few seconds remained visible to the naked eye. The transient was associated with GRB 080319B, a gamma-ray burst at a luminosity distance of about 6 Gpc (standard cosmology), making it the most luminous optical object ever recorded by human kind. We present comprehensive sky monitoring and multi-color optical follow-up observations of GRB 080319B collected by the RAPTOR telescope network covering the development of the explosion and the afterglow before, during, and after the burst. The extremely bright prompt optical emission revealed features that are normally not detectable. The optical and gamma-ray variability during the explosion are correlated, but the optical flux is much greater than can be reconciled with single emission mechanism and a flat gamma-ray spectrum. This extreme optical behavior is best understood as synchrotron self-Compton model (SSC). After a gradual onset of the gamma-ray emission, there is ...

  4. A naked eye aggregation assay for Pb2+ detection based on glutathione-coated gold nanostars

    Science.gov (United States)

    D'Agostino, Agnese; Taglietti, Angelo; Bassi, Barbara; Donà, Alice; Pallavicini, Piersandro

    2014-10-01

    Gold nanostars (AuNS) with a mean hydrodynamic size of 40 nm, obtained with a seed-growth approach using a zwitterionic surfactant (laurylsulfobetaine, LSB), were successfully coated with glutathione (GSH), obtaining a stable and purified solid product which can be easily stored and re-dissolved on need in 0.1 M aqueous solution of Hepes buffered at pH 7. Upon exposure to micromolar concentrations of Pb2+ cation, the GSH-coated nano-objects undergo a fast aggregation followed by sedimentation leading to complete precipitation in about an hour. The subsequent disappearing of the intense LSPR extinction can of course be followed spectrophotometrically but, most importantly, can be easily detected with the naked eye. No signs of this event are noticed when other divalent cations are added to the colloidal suspension in the same condition. A careful investigation was performed to study this selectivity and the behaviour of aggregation as a function of time and Pb2+ cation concentration. We demonstrate that an easy, rapid, instrument-free, visual detection of micromolar levels of Pb2+ is thus possible with this assay, showing a good selectivity towards other investigated metal cations.

  5. [Fluorescence property of a chemical probe for naked-eye and detection of Fe3+].

    Science.gov (United States)

    Song, Yu-Min; Ma, Xin-Xian; Yang, Wu

    2012-12-01

    A higher selective and sensitive probe for the detection of Fe(III) in aqueous media was made using 2,4-Diisocyanatotoluene (TDI) as a bridge to couple Fe3 O4 nanoparticles(NPs) and Rhodamine-6G hydrazide. The characterization of composite materials with Infrared spectra(IR), Thermal Gravimetric analysis(TGA) and Transmission Emission Microscopy(TEM) points to the graft of Rhodamine-6G hydrazide onto the surface of the Fe3O4. The obvious color change of the probe solution from light grey to pink upon the addition of Fe3+ demonstrated the probe could be used for "naked-eye" detection of Fe3+ in water at pH 7. The presence of 1 equivalent (10 micromol x L(-1) microm) of each of these metal ions, including Mn2+, Ni2+, Y2+, Eu3+, Ce3+, La3+, Pr3+, Cd2+, Cr3+, Sm3+, Fe2+, Cu2+ and Zn2+ ions, did not demonstrate any obvious fluorescence change of the probe water solution, which confirmed the probe was a probe with remarkable selectivity for Fe3+. And the fluorescence images of HeLa cells in physiological solutions after incubation with Fe3+ and then further incubated with the probe leading to a strong intracellular fluorescence, which suggested the probe could penetrate the HeLa cell membrane and could respond to Fe3+ in intracellular within living cells.

  6. Structural and optical properties of the naked and passivated Al5Au5 bimetallic nanoclusters

    Science.gov (United States)

    Grande-Aztatzi, Rafael; Formoso, Elena; Mercero, Jose M.; Matxain, Jon M.; Grabowski, Slawomir J.; Ugalde, Jesus M.

    2016-03-01

    The structural and optical properties of both the naked and passivated bimetallic Al5Au5 nanoclusters have been analyzed based on data obtained from ab initio density functional theory and quantum molecular dynamics simulations. It has been found that the Al5Au5 nanocluster possesses a hollow shaped minimum energy structure with segregated Al and Au layered domains, the former representing the electrophilic domain and the latter the nucleophilic domain. In particular, it has been shown that alkali metal cations attach in the nucleophilic domain and hop from one Au site to the next one in the picoseconds time scale, while anions are bound tightly to the Al atoms of the electrophilic domain. Simulating annealing studies are very suggestive of the proneness of the nanocluster towards coalescence into large cluster units, when the cluster is left unprotected by appropriate ligands. Further passivation studies with NaF salt suggest, nonetheless, the possibility of the isolation of the Al5Au5 cluster in molten salts or ionic liquids.

  7. Cosmic censorship and stationary states of half-spin particles in the field of Reissner-Nordström naked singularity

    Science.gov (United States)

    Gorbatenko, M. V.; Neznamov, V. P.; Popov, E. Yu; Safronov, I. I.

    2016-02-01

    The paper explores quantum mechanics of half-spin particle motion in the field of Reissner-Nordström (RN) naked singularity. It is shown that for any quantum mechanical Dirac particle, irrespective of availability and sign of its electrical charge, the RN naked singularity is separated by an infinitely high positive potential barrier. With like charges of a particle and the source of the RN naked singularity, near the origin there exists the second completely impenetrable potential barrier. It has been proved that in the field of the RN naked singularity, bound states of half-spin particles can exist. The conditions for appearance of such states were revealed and computations were performed to find energy eigenvalues and eigenfunctions.

  8. Oxnerella micra sp. n. (Oxnerellidae fam. n.), a tiny naked centrohelid, and the diversity and evolution of heliozoa.

    Science.gov (United States)

    Cavalier-Smith, Thomas; Chao, Ema E

    2012-07-01

    We describe a new tiny naked centrohelid heliozoan, Oxnerella micra, and sequenced its 18S and 28S rRNA genes. Its extremely slender axopodia have prominent extrusomes and are normally stretched across the substratum like those of many tiny granofilosean Cercozoa. Phylogenetic analysis of 18S rDNA shows that Oxnerella does not branch within any of the six known centrohelid families but very deeply in the order Pterocystida, between Choanocystidae and Pterocystidae; therefore we place it in a new family, Oxnerellidae. Oxnerella arose from ancestors with siliceous scales by losing them; as independently did Heterophryidae and Marophryidae, which replaced them by organic spicules, and Chlamydaster that is not truly naked but retains a mucilage coat and nests extremely shallowly within Pterocystidae. 28S rDNA has a group I intron. Concatenated Bayesian 18S/28S rRNA phylogeny shows centrohelids weakly as sisters to the naked non-centrohelid heliozoan Microheliella maris (Microhelida: Heliozoa). The centrohelid Marophrys marina possesses an elongation factor α-like (EFL) protein related to that of Polyplacocystis; Microheliella also has EFL. We also analysed Hsp90 and 18S rDNA sequences from 'Pinaciophora sp.' ATCC50355; they must be from a centrohelid, probably misidentified as Pinaciophora, the rDNA sequence branching deeply within Pterocystida. We reclassify two Polyplacocystis, Luffisphaera, Phaeodaria and Rotosphaerida.

  9. Circular geodesics of naked singularities in the Kehagias-Sfetsos metric of Ho\\v{r}ava's gravity

    CERN Document Server

    Vieira, Ronaldo S S; Kluźniak, W\\lodek; Stuchlík, Zdeněk; Abramowicz, Marek

    2013-01-01

    We discuss photon and test-particle orbits in the Kehagias-Sfetsos (KS) metric. For any value of the Ho\\v{r}ava parameter $\\omega$, there are values of the gravitational mass $M$ for which the metric describes a naked singularity, and this is always accompanied by a vacuum "antigravity sphere" on whose surface a test particle can remain at rest (in a zero angular momentum geodesic), and inside which no circular geodesics exist. The observational appearance of an accreting KS naked singularity in a binary system would be that of a quasi-static spherical fluid shell surrounded by an accretion disk, whose properties depend on the value of $M$, but are always very different from accretion disks familiar from the Kerr-metric solutions. The properties of the corresponding circular orbits are qualitatively similar to those of the Reissner-Nordstr\\"om naked singularities. When event horizons are present, the orbits outside the Kehagias-Sfetsos black hole are qualitatively similar to those of the Schwarzschild metric.

  10. Circular geodesics of naked singularities in the Kehagias-Sfetsos metric of Hořava's gravity

    Science.gov (United States)

    Vieira, Ronaldo S. S.; Schee, Jan; Kluźniak, Włodek; Stuchlík, Zdeněk; Abramowicz, Marek

    2014-07-01

    We discuss photon and test-particle orbits in the Kehagias-Sfetsos (KS) metric of Hořava's gravity. For any value of the Hořava parameter ω, there are values of the gravitational mass M for which the metric describes a naked singularity, and this is always accompanied by a vacuum "antigravity sphere" on whose surface a test particle can remain at rest (in a zero angular momentum geodesic), and inside which no circular geodesics exist. The observational appearance of an accreting KS naked singularity in a binary system would be that of a quasistatic spherical fluid shell surrounded by an accretion disk, whose properties depend on the value of M, but are always very different from accretion disks familiar from the Kerr-metric solutions. The properties of the corresponding circular orbits are qualitatively similar to those of the Reissner-Nordström naked singularities. When event horizons are present, the orbits outside the Kehagias-Sfetsos black hole are qualitatively similar to those of the Schwarzschild metric.

  11. Physical properties of naked DNA influence nucleosome positioning and correlate with transcription start and termination sites in yeast

    Directory of Open Access Journals (Sweden)

    Soler-López Montserrat

    2011-10-01

    Full Text Available Abstract Background In eukaryotic organisms, DNA is packaged into chromatin structure, where most of DNA is wrapped into nucleosomes. DNA compaction and nucleosome positioning have clear functional implications, since they modulate the accessibility of genomic regions to regulatory proteins. Despite the intensive research effort focused in this area, the rules defining nucleosome positioning and the location of DNA regulatory regions still remain elusive. Results Naked (histone-free and nucleosomal DNA from yeast were digested by microccocal nuclease (MNase and sequenced genome-wide. MNase cutting preferences were determined for both naked and nucleosomal DNAs. Integration of their sequencing profiles with DNA conformational descriptors derived from atomistic molecular dynamic simulations enabled us to extract the physical properties of DNA on a genomic scale and to correlate them with chromatin structure and gene regulation. The local structure of DNA around regulatory regions was found to be unusually flexible and to display a unique pattern of nucleosome positioning. Ab initio physical descriptors derived from molecular dynamics were used to develop a computational method that accurately predicts nucleosome enriched and depleted regions. Conclusions Our experimental and computational analyses jointly demonstrate a clear correlation between sequence-dependent physical properties of naked DNA and regulatory signals in the chromatin structure. These results demonstrate that nucleosome positioning around TSS (Transcription Start Site and TTS (Transcription Termination Site (at least in yeast is strongly dependent on DNA physical properties, which can define a basal regulatory mechanism of gene expression.

  12. DA DIALÉTICA DA INTOXICAÇÃO EM NAKED LUNCH

    Directory of Open Access Journals (Sweden)

    Fábio Ackcelrud Durão

    2014-09-01

    Full Text Available A fama de Naked Lunch (1959, de William Burroughs pode ser explicada tanto por seu conteúdo quanto forma: a a descrição chocante de todo tipo de obscenidade e abjeção; b seu experimentalismo, por meio de um uso extenso de uma técnica de ruptura, associada a uma escrita modernista transgressiva. O presente ensaio discute a adaptação de David Cronenberg, investigando recursos que favorecem um sentido mais simples, negado pelo livro, porém fundamental para garantir a circulação de um produto da indústria cultural. Procura-se demonstrar que, se a obra de Burroughs é regida pelo dito “nada é verdadeiro, tudo permitido”, a versão fílmica de Cronenberg usa a defesa da intoxicação, não para alcançar um processo verdadeiramente criativo, mas para desenvolver uma estrutura narrativa que inverte a oposição convencional entre alucinação e sobriedade. O resultado é uma tensão na qual os estados alucinatórios encontram-se plasmados por gêneros narrativos do cinema mainstream, como o noir e o filme de conspiração. É na alucinação quese forma um padrão narrativo coeso, que só é desestabilizado por pequenas interferências que podem ser vistas como erros técnicos. A estrutura narrativa alucinatória é construída com base em uma naturalização coerciva da estética cinematográfica análoga aos procedimentos de homogeneização da percepção da indústria cultural.

  13. Serum-stabilized naked caspase-3 siRNA protects autotransplant kidneys in a porcine model.

    Science.gov (United States)

    Yang, Cheng; Zhao, Tian; Zhao, Zitong; Jia, Yichen; Li, Long; Zhang, Yufang; Song, Mangen; Rong, Ruiming; Xu, Ming; Nicholson, Michael L; Zhu, Tongyu; Yang, Bin

    2014-10-01

    The naked small interfering RNA (siRNA) of caspase-3, a key player in ischemia reperfusion injury, was effective in cold preserved and hemoreperfused kidneys, but not autotransplanted kidneys in our porcine models. Here, chemically modified serum stabilized caspase-3 siRNAs were further evaluated. The left kidney was retrieved and infused by University of Wisconsin solution with/without 0.3 mg caspase-3 or negative siRNA into the renal artery for 24-hour cold storage (CS). After an intravenous injection of 0.9 mg siRNA and right-uninephrectomy, the left kidney was autotransplanted for 2 weeks. The effectiveness of caspase-3 siRNA was confirmed by caspase-3 knockdown in the post-CS and/or post-transplant kidneys with reduced apoptosis and inflammation, while the functional caspase-3 siRNA in vivo was proved by detected caspase-3 mRNA degradation intermediates. HMGB1 protein was also decreased in the post-transplanted kidneys; correlated positively with renal IL-1β mRNA, but negatively with serum IL-10 or IL-4. The minimal off-target effects of caspase-3 siRNA were seen with favorable systemic responses. More importantly, renal function, associated with active caspase-3, HMGB1, apoptosis, inflammation, and tubulointerstitial damage, was improved by caspase-3 siRNA. Taken together, the 2-week autotransplanted kidneys were protected when caspase-3 siRNA administrated locally and systemically, which provides important evidence for future clinical trials.

  14. Effects of Cultivar on Phenolic Content and Antioxidant Activity of Naked Oat in China

    Institute of Scientific and Technical Information of China (English)

    TONG Li-tao; LIU Li-ya; ZHONG Kui; WANG Yan; GUO Li-na; ZHOU Su-mei

    2014-01-01

    To evaluate the effects of naked oat cultivars on their phenolic contents and antioxidant activities, the oat seeds from 21 different cultivars in China were collected, and their major nutritional components, compositions of polyphenols, and antioxidant activities were measured. As the results, oat cultivars affected its phenolic composition and antioxidant activity signiifcantly. Vanillin, chlorogenic acid, ferulic acid, avenanthramide C (Bc), avenanthramide A (Bp), and avenanthramide B (Bf) in oat polyphenols extracts were detected in all cultivars of oat, while caffeic acid, p-coumaric acid and rutin were partly detected. All oat cultivars showed signiifcant free radical scavenging activity, although their activities were lower than that of vitamin C (VC). Different oat varieties showed different antioxidant activities, among which Yanke 1 had the best ability to scavenge•OH ((58.78±1.99)μg mL-1), Baiyan 10 showed the strongest DPPH•((22.00±0.43)μg mL-1) and ABTS•+((6.92±0.44)μg mL-1) scavenging activity, and Dingyou 1 showed good scavenging ability of DPPH•((22.71±0.42)μg mL-1) and•OH ((81.50±1.73)μg mL-1). In addition, the correlation coefifcients of contents between the major nutritional components in different cultivars of oat and phenolic compounds indicated that determination of main nutrients could be used as an easy technique to evaluate approximately the contents and compositions of oat polyphenols, which simpliifes the selection of good oat cultivar.

  15. THE NAKED EMERGENCE OF SOLAR ACTIVE REGIONS OBSERVED WITH SDO/HMI

    Energy Technology Data Exchange (ETDEWEB)

    Centeno, Rebecca [High Altitude Observatory (NCAR), 3080 Center Green Dr., Boulder, CO 80301 (United States)

    2012-11-01

    We take advantage of the HMI/SDO instrument to study the naked emergence of active regions (ARs) from the first imprints of the magnetic field on the solar surface. To this end, we followed the first 24 hr in the life of two rather isolated ARs that appeared on the surface when they were about to cross the central meridian. We analyze the correlations between Doppler velocities and the orientation of the vector magnetic field, consistent finding that the horizontal fields connecting the main polarities are dragged to the surface by relatively strong upflows and are associated with elongated granulation that is, on average, brighter than its surroundings. The main magnetic footpoints, on the other hand, are dominated by vertical fields and downflowing plasma. The appearance of moving dipolar features (MDFs, of opposite polarity to that of the AR) in between the main footpoints is a rather common occurrence once the AR reaches a certain size. The buoyancy of the fields is insufficient to lift up the magnetic arcade as a whole. Instead, weighted by the plasma that it carries, the field is pinned down to the photosphere at several places in between the main footpoints, giving life to the MDFs and enabling channels of downflowing plasma. MDF poles tend to drift toward each other, merge and disappear. This is likely to be the signature of a reconnection process in the dipped field lines, which relieves some of the weight allowing the magnetic arcade to finally rise beyond the detection layer of the Helioseismic and Magnetic Imager spectral line.

  16. Regional differences in the surface temperature of Naked Neck laying hens in a semi-arid environment

    Science.gov (United States)

    de Souza, João Batista Freire; de Arruda, Alex Martins Varela; Domingos, Hérica Girlane Tertulino; de Macedo Costa, Leonardo Lelis

    2013-05-01

    The aim of this study was to evaluate the regional differences in the surface temperature of Naked Neck hens that were subjected to different temperatures in a semi-arid environment. The surface temperature was measured in four body regions (face, neck, legs and feathered area) of 60 Naked Neck hens. The following environmental variables were measured at the center of the shed: the black globe temperature ( T G ), air temperature ( T A ), wind speed ( U) and relative humidity ( R H ). The T A was divided into three classes: 1 (24.0-26.0 °C), 2 (26.1-28.9 °C) and 3 (29.0-31.0 °C). An analysis of variance was performed by the least squares method and a comparison of the means by the Tukey-Kramer test. The results showed a significant effect of T A class, the body region and the interaction between these two effects on the surface temperature. There was no significant difference between the T A classes for the face and neck. The legs and feathered area showed significant differences between the T A classes. Regarding the effect of body regions within each T A class, there was a significant difference among all regions in the three T A classes. In all T A classes the neck had the highest average followed by the face and legs. The feathered area showed the lowest average of the different T A classes. In conclusion, this study showed that there are regional differences in the surface temperature of Naked Neck hens, with the legs acting as thermal windows.

  17. Naked eye instant reversible sensing of Cu(2+) and its in situ imaging in live brine shrimp Artemia.

    Science.gov (United States)

    Nair, Ratish R; Raju, M; Patel, Neha P; Raval, Ishan H; Suresh, E; Haldar, Soumya; Chatterjee, Pabitra B

    2015-08-21

    A Cu(2+)-specific colorimetric reversible fluorescent receptor was designed and synthesized which showed a naked eye observable colour change from colourless to pink on addition of an aqueous buffer (pH 7.4) solution of 30 ppb Cu(2+). Short response time (≤5 s) and low detection limit (nearly 3 ppb) make suitable as a reliable "dip-in" open eye sensor for Cu(2+). Bio-imaging application in live brine shrimp Artemia enabled to detect Cu(2+) at as low as 10 ppb exposure.

  18. Non-radial strong curvature naked singularities in five dimensional perfect fluid self-similar space-time

    CERN Document Server

    Sarwe, S B; Sarwe, Sanjay B.

    2004-01-01

    We study five dimensional(5D) spherically symmetric self-similar perfect fluid space-time with adiabatic equation of state, considering all the families of future directed non-spacelike geodesics. The space-time admits globally strong curvature naked singularities in the sense of Tipler and thus violates the cosmic censorship conjecture provided a certain algebraic equation has real positive roots. We further show that it is the weak energy condition (WEC) that is necessary for visibility of singularities for a finite period of time and for singularities to be gravitationally strong. We, also, match the solution to 5D Schwarzschild solution using the junction conditions.

  19. Non-parametric Data Analysis of Low-latitude Auroras and Naked-eye Sunspots in the Medieval Epoch

    Science.gov (United States)

    Bekli, Mohamed Reda; Zougab, Nabil; Belabbas, Abdelmoumene; Chadou, Ilhem

    2017-04-01

    We have studied solar activity by analyzing naked-eye sunspot observations and aurorae borealis observed at latitudes below 45°. We focused on the medieval epoch by considering the non-telescopic observations of sunspots from AD 974 to 1278 and aurorae borealis from AD 965 to 1273 that are reported in several Far East historical sources, primarily in China and Korea. After setting selection rules, we analyzed the distribution of these individual events following the months of the Gregorian calendar. In December, an unusual peak is observed with data recorded in both China and Japan, but not within Korean data.

  20. THE “NAKED FACE” OF SECULAR EXCLUSION: BILL 94 AND THE PRIVATIZATION OF BELIEF

    Directory of Open Access Journals (Sweden)

    Pascale Fournier

    2012-02-01

    Full Text Available This article will consider the case study of Québec’s Bill 94 (An Act to establish guidelines governing accommodation requests within the Administration and certain institutions, introduced in March 2010, one of many recent bans imposed on the wearing of the niqab in the West. Citing the importance of “the right to gender equality and the principle of religious neutrality of the State,” Bill 94 emphasizes the necessity of “un visage découvert” or “naked face” when giving or receiving a broad range of public services in Québec, including all government services, childcare centres, hospitals, and health and social service agencies. According to section 6 of the Act, “If an accommodation involves an adaptation of that practice and reasons of security, communication or identification warrant it, the accommodation must be denied.” While quasi-neutral, this bill clearly has a disproportionate impact on religious women who wear the niqab. In fact, as a direct result of the legislation, Muslim women will likely disappear from the public sphere and be restricted to the private home where they might effectively be dependent on male family members to navigate the “market place” on their behalf. Borrowing from Charles Taylor’s A Secular Age, this paper will consider the distributive consequences of the niqab ban, a critical juncture of “religion-state relations” in which belief is more and more relegated to the private sphere in Quebec. The article will use Bill 94 to explore this peculiar manifestation of “secularism” with the concurrent existence of “governance feminism”— how the privatization of belief goes hand in hand and is perversely reinforced by a colonial discourse on gender equality, leaving some already marginalized women out of the public gaze. Is this legislated demand for a “naked face” truly the logical outcome of a successful feminist movement (as some have asserted or isthis erasure of religious

  1. Uma Vida (nua como Piscina (sem gua? Is a (naked Life like a(n (empty Swimmingpool?

    Directory of Open Access Journals (Sweden)

    Rogrio da Costa

    2012-01-01

    Full Text Available Este artigo discute o conceito de uma vida, proposto por Gilles Deleuze ao longo de vrios momentos de sua obra e, sobretudo, naquele que considerado seu ltimo texto, LImmanence: une vie Coloca-o tambm em contato com a interpretao tentada por Agamben, que o faz atravs da aproximao de uma vida ao conceito de vida nua. Finalmente, introduz uma variante, o conceito de vida despida, que funciona como uma espcie de passagem entre a vida nua de Agamben e uma vida de Deleuze.This article discusses the concept of a life, proposed by Gilles Deleuze over several times in his work and, overall, in what is considered his last text, LImmanence: une vie ... It also places him in touch with interpretation tried by Agamben, through the approximation of a life into the concept of naked life. Finally, it introduces the concept of bare life, a variant that functions as some sort of passage between Agambens naked life and Deleuzes a life.

  2. Novel signal-amplifying fluorescent nanofibers for naked-eye-based ultrasensitive detection of buried explosives and explosive vapors

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Ying; La, Anthony; Ding, Yu; Liu, Yixin; Lei, Yu [Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, CT (United States)

    2012-09-11

    A novel electrospun fluorescent nanofiberous membrane with a function like ''molecular wires'' was developed via electrospinning for the detection of ultra-trace nitro explosive vapors and buried explosives by naked eye under UV excitation. The high binding affinity between the electron-deficient nitro explosives and the sensing film results in a rapid, dramatic quenching in its fluorescence emission. A wide spectrum of nitro explosives, in particular, TNT, Tetryl, RDX, PETN and HMX could be ''visually'' detected at their sub-equilibrium vapors (less than 10 ppb, 74 ppt, 5 ppt, 7 ppt and 0.1 ppt, respectively) released from 1 ng explosives residues. Such outstanding sensing performance could be attributed to the proposed ''sandwich-like'' conformation between pyrene and phenyl pendants of PS which may allow efficient long-range energy migration similar to ''molecular wire'', thus achieving amplified fluorescence quenching. Its application for the detection of buried explosives in soil by naked eye was also demonstrated, indicating its potential application for landmine mapping. To the best of our knowledge, this is the first report about the detection of buried explosives without the use of any advanced analytical instrumentation. (Copyright copyright 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  3. Naked-eye detection and thermochromic properties of Cu(II)-3,3'-thiodipropionate complexes with benzimidazole.

    Science.gov (United States)

    Arıcı, Mürsel; Yeşilel, Okan Zafer; Taş, Murat

    2015-01-28

    Two new coordination complexes, namely, [Cu(tdp)(H2O)(bim)3]·4H2O (1) and {[Cu(μ2-tdp)(bim)2]·4H2O}n (2) (tdp = 3,3'-thiodipropionate, bim = benzimidazole), having naked-eye sensor properties and thermochromic behaviors, were synthesized and structurally characterized using elemental analysis, IR and UV spectra, and single-crystal X-ray diffraction, powder X-ray diffraction (PXRD) and thermal analyses (TG, DTA and DTG) techniques. Complex 1 changed color from blue to dark and light green in methanol and DMF solvents, respectively, while complex 2 changed color from light blue to light green only in DMF solvent. Moreover, complex 1 can be used to detect as little as 10 percent methanol in ethanol by the naked eye. The thermochromic properties of the complexes showed that complexes 1 and 2 changed color from blue and light blue to light and dark green at 65 °C, respectively.

  4. DNA repair in species with extreme lifespan differences

    Science.gov (United States)

    MacRae, Sheila L.; Croken, Matthew McKnight; Calder, R.B.; Aliper, Alexander; Milholland, Brandon; White, Ryan R.; Zhavoronkov, Alexander; Gladyshev, Vadim N.; Seluanov, Andrei; Gorbunova, Vera; Zhang, Zhengdong D.; Vijg, Jan

    2015-01-01

    Differences in DNA repair capacity have been hypothesized to underlie the great range of maximum lifespans among mammals. However, measurements of individual DNA repair activities in cells and animals have not substantiated such a relationship because utilization of repair pathways among animals—depending on habitats, anatomical characteristics, and life styles—varies greatly between mammalian species. Recent advances in high-throughput genomics, in combination with increased knowledge of the genetic pathways involved in genome maintenance, now enable a comprehensive comparison of DNA repair transcriptomes in animal species with extreme lifespan differences. Here we compare transcriptomes of liver, an organ with high oxidative metabolism and abundant spontaneous DNA damage, from humans, naked mole rats, and mice, with maximum lifespans of ∼120, 30, and 3 years, respectively, with a focus on genes involved in DNA repair. The results show that the longer-lived species, human and naked mole rat, share higher expression of DNA repair genes, including core genes in several DNA repair pathways. A more systematic approach of signaling pathway analysis indicates statistically significant upregulation of several DNA repair signaling pathways in human and naked mole rat compared with mouse. The results of this present work indicate, for the first time, that DNA repair is upregulated in a major metabolic organ in long-lived humans and naked mole rats compared with short-lived mice. These results strongly suggest that DNA repair can be considered a genuine longevity assurance system. PMID:26729707

  5. Dose-dependent Cytotoxicity and Oxidative Stress Induced by "Naked" Fe3O4 Nanoparticles in Human Hepatocyte

    Institute of Scientific and Technical Information of China (English)

    LIN Xin-li; ZHAO Shu-hua; ZHANG Long; HU Gui-qin; SUN Zhi-wei; YANG Wen-sheng

    2012-01-01

    The dose-dependent cytotoxicity effect on human hepatocyte(HL-7702 cells) induced by "naked" Fe3O4 nanoparticles was assessed through cell viabilities and lactate dehydrogenase(LDH) activities.Three important oxidative indexes of the cells by glutathione peroxidase(GSH-Px),superoxide dismutase(SOD) and malondialdehyde(MDA) were determined.The good correlation of the cell viabilities with their GSH-Px,SOD and MDA levels indicated that the cytotoxicity is related to activation of oxidative stress induced by Fe3O4 nanoparticles.The oxidative stress also leads to corresponding DNA damage in a similar dose-dependent manner,followed by the changes of cell cycle and cell apoptosis.Such work provides important experimental data for the safety evaluation of superparamagnetic Fe3O4 nanoparticles.

  6. Constraints on the optical precursor to the naked-eye burst GRB080319B from Pi of the Sky observations

    CERN Document Server

    Piotrowski, Lech Wiktor

    2012-01-01

    I present the results of the search for an optical precursor to the naked-eye burst - GRB080319B, which reached 5.87m optical peak luminosity in the "Pi of the Sky" data. A burst of such a high brightness could have been preceded by an optical precursor luminous enough to be in detection range of our experiment. The "Pi of the Sky" cameras observed the coordinates of the GRB for about 20 minutes prior to the explosion, thus provided crucial data for the precursor search. No signal within 3 sigma limit was found. A limit of 12m (V-band equivalent) was set based on the data combined from two cameras, the most robust limit to my knowledge for this precursor.

  7. Serum Haemolytic Complement Levels in German Dahlem Red Chickens Are Affected by Three Major Genes (Naked, Neck, Dwarf, Frizzled) of Tropical Interest

    NARCIS (Netherlands)

    Dorny, P.; Baelmans, R.; Parmentier, H.K.; Nieuwland, M.G.B.; Demey, F.; Berkvens, D.

    2005-01-01

    German Dahlem Red chickens with three different major genes of tropical interest: Nana ¿ (naked neck), Ff ¿ (frizzled) and dw ¿ (dwarf), respectively, were tested for serum haemolytic complement, which is essential in innate host defence against infectious agents. Eight different combinations of gen

  8. On the variably-charged black holes in general relativity: Hawking's radiation and naked singularities

    Energy Technology Data Exchange (ETDEWEB)

    Ibohal, Ng [Department of Mathematics, Manipur University, Imphal 795003, Manipur (India)

    2002-08-21

    In this paper variably-charged non-rotating Reissner-Nordstrom and rotating Kerr-Newman black holes are discussed. Such a variable charge e with respect to the polar coordinate r in the field equations is referred to as an electrical radiation of the black hole. It is shown that every electrical radiation e(r) of the non-rotating black hole leads to a reduction in its mass M by some quantity. If one considers such electrical radiation taking place continuously for a long time, then a continuous reduction of the mass may take place in the black-hole body and the original mass of the black hole may be evaporated completely. At that stage, the gravity of the object may depend only on the electromagnetic field, not on the mass. Immediately after the complete evaporation of the mass, if the next radiation continues, there may be creation of a new mass leading to the formation of a negative mass naked singularity. It appears that this new mass of the naked singularity would never decrease, but might increase gradually as the radiation continues forever. A similar investigation is also discussed in the case of a variably-charged rotating Kerr-Newman black hole. Thus, it has been shown by incorporating Hawking's evaporation of radiating black holes in the form of spacetime metrics, every electrical radiation of variably-charged rotating and non-rotating black holes may produce a change in the mass of the body without affecting the Maxwell scalar.

  9. ENERGETIC VALUE OF FORAGES FROM SEMI-ARID REGION AND DIGESTIBILITY OF RATIONS FOR NAKED NECK PULLETS

    Directory of Open Access Journals (Sweden)

    ALEX MARTINS VARELA DE ARRUDA

    2014-01-01

    Full Text Available The feeding programs for naked neck chickens in semi-intensive production system from brazilian equatorial semi-arid environment, must consider regional food availability and respective nutritional values. Thus, to evaluate the digestibility and metabolizable energy of alternative forages, it was used 240 naked neck pullets (Isa Label lineage receiving water and ration ad libitum, pair-housed in cages for total collection of excreta on conventional warehouse. It was used a completely randomized design with factorial arrangement (5x2: one control ration (corn and soy meal and other four experimental rations with silk flower hay (Calotropis procera, cassava leafs hay (Manihot esculenta, kills pasture hay (Senna obtusifolia or leucaena leafs hay (Leucaena leucocephala, and all rations were balanced for two growing phases, between 8 and 10 weeks (young pullets and between 14 and 16 weeks of age (old pullets. The values of apparent digestibility of nutrients for all experimental rations were lower than control ration (P <0.05 and it was observed general means of 72.18% for dry matter, 78.12% for crude protein, 66.90% for ether extract, 28.08% for neutral detergent fiber, 18.51% for the acid detergent fiber, 71.64% for gross energy and availability of 15.61% for mineral matter. The general mean of apparent and corrected metabolizable energy of alternative forages was 1217 kcal/ kg and 1108 kcal/kg, respectively, and the higher value was determined for leucaena hay and the lower value for silk flower hay (P <0.05.

  10. Uptake of synthetic naked RNA by skin-resident dendritic cells via macropinocytosis allows antigen expression and induction of T-cell responses in mice.

    Science.gov (United States)

    Selmi, Abderraouf; Vascotto, Fulvia; Kautz-Neu, Kordula; Türeci, Özlem; Sahin, Ugur; von Stebut, Esther; Diken, Mustafa; Kreiter, Sebastian

    2016-09-01

    Intradermal administration of antigen-encoding RNA has entered clinical testing for cancer vaccination. However, insight into the underlying mechanism of RNA uptake, translation and antigen presentation is still limited. Utilizing pharmacologically optimized naked RNA, the dose-response kinetics revealed a rise in reporter signal with increasing RNA amounts and a prolonged RNA translation of reporter protein up to 30 days after intradermal injection. Dendritic cells (DCs) in the dermis were shown to engulf RNA, and the signal arising from the reporter RNA was significantly diminished after DC depletion. Macropinocytosis was relevant for intradermal RNA uptake and translation in vitro and in vivo. By combining intradermal RNA vaccination and inhibition of macropinocytosis, we show that effective priming of antigen-specific CD8(+) T-cells also relies on this uptake mechanism. This report demonstrates that direct antigen translation by dermal DCs after intradermal naked RNA vaccination is relevant for efficient priming of antigen-specific T-cells.

  11. Interaction of Thermus thermophilus, ArsC enzyme and gold nanoparticles naked-eye assays speciation between As(III) and As(V)

    Science.gov (United States)

    Politi, Jane; Spadavecchia, Jolanda; Fiorentino, Gabriella; Antonucci, Immacolata; Casale, Sandra; De Stefano, Luca

    2015-10-01

    The thermophilic bacterium Thermus thermophilus HB27 encodes chromosomal arsenate reductase (TtArsC), the enzyme responsible for resistance to the harmful effects of arsenic. We report on adsorption of TtArsC onto gold nanoparticles for naked-eye monitoring of biomolecular interaction between the enzyme and arsenic species. Synthesis of hybrid biological-metallic nanoparticles has been characterized by transmission electron microscopy (TEM), ultraviolet-visible (UV-vis), dynamic light scattering (DLS) and phase modulated infrared reflection absorption (PM-IRRAS) spectroscopies. Molecular interactions have been monitored by UV-vis and Fourier transform-surface plasmon resonance (FT-SPR). Due to the nanoparticles’ aggregation on exposure to metal salts, pentavalent and trivalent arsenic solutions can be clearly distinguished by naked-eye assay, even at 85 μM concentration. Moreover, the assay shows partial selectivity against other heavy metals.

  12. Dynamics of current-carrying string loops in the Kerr naked-singularity and black-hole spacetimes

    CERN Document Server

    Kološ, M

    2013-01-01

    Current-carrying string-loop dynamics is studied in the Kerr spacetimes. With attention concentrated to the axisymmetric motion of string loops around symmetry axis of both black-hole (BH) and naked singularity (NS) spacetimes, it is shown that the resulting motion is governed by the presence of an outer tension barrier and an inner angular momentum barrier that are influenced by the BH or NS spin. We classify the string dynamics according to properties of the energy boundary function (effective potential) for the string loop motion. We have found that for NS there exist new types of energy boundary function, namely those with off-equatorial minima. Conversion of the energy of the string oscillations to the energy of the linear translational motion has been studied. Such a transmutation effect is much more efficient in the NS spacetimes because lack of the event horizon. For BH spacetimes efficiency of the transmutation effect is only weakly spin dependent. Transition from regular to chaotic regime of the str...

  13. Indeterministic Quantum Gravity and Cosmology; 7, Dynamical Passage through Singularities Black Hole and Naked Singularity, Big Crunch and Big Bang

    CERN Document Server

    Mashkevich, V S

    1997-01-01

    This paper is a continuation of the papers [gr-qc/9409010, gr-qc/9505034, gr-qc/9603022, gr-qc/9609035, gr-qc/9609046, gr-qc/9704033]. The aim of the paper is to incorporate singularities---both local (black hole and naked singularity) and global (big bang and big crunch)---into the dynamics of indeterministic quantum gravity and cosmology. The question is whether a singularity is dynamically passable, i.e., whether a dynamical process which ends with a singularity may be extended beyond the latter. The answer is yes. A local singularity is trivially passable, while the passableness for a global singularity may invoke CPT transformation. The passableness of the singularities implies pulsating black holes and the oscillating universe. For the local singularity, the escape effect takes place: In a vicinity of the singularity, quantum matter leaves the gravitational potential well. Keywords: tempered singularity, strong singularity, trivial passage, CPT passage, pulsating black hole, escape effect, oscillating u...

  14. Fast Optical Variability of a Naked-eye Burst—Manifestation of the Periodic Activity of an Internal Engine

    Science.gov (United States)

    Beskin, G.; Karpov, S.; Bondar, S.; Greco, G.; Guarnieri, A.; Bartolini, C.; Piccioni, A.

    2010-08-01

    We imaged the position of the naked-eye burst, GRB080319B, before, during, and after its gamma-ray activity with sub-second temporal resolution using the TORTORA wide-field camera. The burst optical prompt emission, which reached 5.3 mag, has been detected, and its periodic optical variability has been discovered in the form of four equidistant flashes with a duration of several seconds. We also detected a strong correlation (r ≈ 0.82) between optical and gamma-ray light curves with a 2 s delay of the optical emission with respect to the gamma-ray emission. The revealed temporal structure of the optical light curve in comparison with the gamma-ray light curve can be interpreted in the framework of the model of shell collisions in the ejecta containing a significant neutron component. All observed emission features reflect the non-stationary behavior of the burst internal engine—supposedly, a hyperaccreting solar-mass black hole formed in the collapse of a massive stellar core.

  15. Distinguishing Kerr naked singularities and black holes using the spin precession of a test gyro in strong gravitational fields

    CERN Document Server

    Chakraborty, Chandrachur; Kocherlakota, Prashant; Bhattacharyya, Sudip; Joshi, Pankaj S; Królak, Andrzej

    2016-01-01

    We consider here the precession of a test gyroscope in Kerr spacetimes to distinguish a naked singularity (NS) from black hole (BH). It is known that when the angular velocity of the gyro vanishes the spin precession frequency diverged at the ergo-surface. We show that it is possible to overcome this divergence by moving the gyro to the ergoregion with a non-zero angular velocity ($\\Omega_e$) in a definite range. Then the frequency is finite and regular on the boundary of the ergoregion and inside, for both the BH and NS. Specifically, if we move the gyro with a non-zero $\\Omega_e$ to an unknown astrophysical object, its precession diverges on the event horizon for a black hole, but finite and regular for NS. Therefore a genuine detection for the existence or otherwise of the event horizon becomes possible. We also show that for a near-extremal ($1 1.089$. For $1

  16. Performance comparison of dwarf laying hens segregating for the naked neck gene in temperate and subtropical environments

    Directory of Open Access Journals (Sweden)

    Bordas André

    2009-01-01

    Full Text Available Abstract This study compares laying performances between two environments of dwarf laying hen lines segregating for the naked neck mutation (NA locus, a selected dwarf line of brown-egg layers and its control line. Layers with one of the three genotypes at the NA locus were produced from 11 sires from the control line and 12 sires from the selected line. Two hatches produced 216 adult hens in Taiwan and 297 hens in France. Genetic parameters for laying traits were estimated in each environment and the ranking of sire breeding values was compared between environments. Laying performance was lower, and mortality was higher in Taiwan than in France. The line by environment interaction was highly significant for body weight at 16 weeks, clutch length and egg number, with or without Box-Cox transformation. The selected line was more sensitive to environmental change but in Taiwan it could maintain a higher egg number than the control line. Estimated heritability values in the selected line were higher in France than in Taiwan, but not for all the traits in the control line. The rank correlations between sire breeding values were low within the selected line and slightly higher in the control line. A few sire families showed a good ranking in both environments, suggesting that some families may adapt better to environmental change.

  17. Synthesis and evaluation of simple naked-eye colorimetric chemosensors for anions based on azo dye-thiosemicarbazones.

    Science.gov (United States)

    Radchatawedchakoon, Widchaya; Sangsuwan, Withsakorn; Kruanetr, Senee; Sakee, Uthai

    2014-01-01

    A series of novel, highly selective azo dye-thiosemicarbazones based anion sensors (3e-f) have been synthesized from the condensation reaction between thiosemicarbazide and six different azo salicylaldehydes. The structure of the sensors was confirmed by spectroscopic methods. The selectivity and sensitivity in the recognition for acetate anion over other anions such as fluoride, chloride, iodide and dihydrogenphosphate anions were determined by naked-eyes and UV-vis spectra. The color of the solution containing sensor had an obvious change from light yellow to orange only after the addition of acetate anion in aqueous solution (water/dimethylsulfoxide, 7:3, v/v) while other anions did not cause obvious color change. The anion recognition property of the receptor via proton-transfer is monitored by UV-vis titration and (1)H NMR spectroscopy. Under condition in aqueous solution of sensor 3e (water/dimethylsulfoxide, 7:3, v/v), linearity range for the quantification of acetate anion was 1-22 μM and limit of detection (LOD) of acetate anion was 0.71 μM.

  18. Rheological and Pasting Properties of Naked Barley Flour as Modified by Guar, Xanthan, and Locust Bean Gums

    Science.gov (United States)

    Yoon, Sung-Jin; Lee, Youngseung; Yoo, Byoungseung

    2016-01-01

    To understand the effects of adding different gums (guar, xanthan, and locust bean gums) on naked barley flour (NBF), the rheological and pasting properties of NBF-gum mixtures were measured at different gum concentrations (0, 0.3, and 0.6% w/w). Steady shear rheological properties were determined by rheological parameters for power law and Casson models. All samples showed a clear trend of shear-thinning behavior (n=0.16~0.48) and had a non-Newtonian nature with yield stress. Consistency index, apparent viscosity, and yield stress values increased with an increase in gum concentration. Storage modulus values were more predominant than loss modulus values with all concentrations of gums. There is a more pronounced synergistic effect of elastic properties of NBF in the presence of xanthan gum. Rapid visco analyser pasting properties showed that the addition of gums resulted in a significant increase in the peak, breakdown, setback, and final viscosities, whereas the pasting temperature decreased. PMID:28078260

  19. Mutations in the human naked cuticle homolog NKD1 found in colorectal cancer alter Wnt/Dvl/beta-catenin signaling.

    Directory of Open Access Journals (Sweden)

    Jianhui Guo

    Full Text Available BACKGROUND: Mutation of Wnt signal antagonists Apc or Axin activates beta-catenin signaling in many cancers including the majority of human colorectal adenocarcinomas. The phenotype of apc or axin mutation in the fruit fly Drosophila melanogaster is strikingly similar to that caused by mutation in the segment-polarity gene, naked cuticle (nkd. Nkd inhibits Wnt signaling by binding to the Dishevelled (Dsh/Dvl family of scaffold proteins that link Wnt receptor activation to beta-catenin accumulation and TCF-dependent transcription, but human NKD genes have yet to be directly implicated in cancer. METHODOLOGY/PRINCIPAL FINDINGS: We identify for the first time mutations in NKD1--one of two human nkd homologs--in a subset of DNA mismatch repair-deficient colorectal tumors that are not known to harbor mutations in other Wnt-pathway genes. The mutant Nkd1 proteins are defective at inhibiting Wnt signaling; in addition, the mutant Nkd1 proteins stabilize beta-catenin and promote cell proliferation, in part due to a reduced ability of each mutant Nkd1 protein to bind and destabilize Dvl proteins. CONCLUSIONS/SIGNIFICANCE: Our data raise the hypothesis that specific NKD1 mutations promote Wnt-dependent tumorigenesis in a subset of DNA mismatch-repair-deficient colorectal adenocarcinomas and possibly other Wnt-signal driven human cancers.

  20. Uptake, Efficacy, and Systemic Distribution of Naked, Inhaled Short Interfering RNA (siRNA) and Locked Nucleic Acid (LNA) Antisense

    Science.gov (United States)

    Moschos, Sterghios A; Frick, Manfred; Taylor, Bruce; Turnpenny, Paul; Graves, Helen; Spink, Karen G; Brady, Kevin; Lamb, David; Collins, David; Rockel, Thomas D; Weber, Markus; Lazari, Ovadia; Perez-Tosar, Luis; Fancy, Sally A; Lapthorn, Chris; Green, Martin X; Evans, Steve; Selby, Matthew; Jones, Gareth; Jones, Lyn; Kearney, Sarah; Mechiche, Houria; Gikunju, Diana; Subramanian, Romesh; Uhlmann, Eugen; Jurk, Marion; Vollmer, Jörg; Ciaramella, Giuseppe; Yeadon, Michael

    2011-01-01

    Antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) promise specific correction of disease-causing gene expression. Therapeutic implementation, however, has been forestalled by poor delivery to the appropriate tissue, cell type, and subcellular compartment. Topical administration is considered to circumvent these issues. The availability of inhalation devices and unmet medical need in lung disease has focused efforts in this tissue. We report the development of a novel cell sorting method for quantitative, cell type-specific analysis of siRNA, and locked nucleic acid (LNA) ASO uptake and efficacy after intratracheal (i.t.) administration in mice. Through fluorescent dye labeling, we compare the utility of this approach to whole animal and whole tissue analysis, and examine the extent of tissue distribution. We detail rapid systemic access and renal clearance for both therapeutic classes and lack of efficacy at the protein level in lung macrophages, epithelia, or other cell types. We nevertheless observe efficient redirection of i.t. administered phosphorothioate (PS) LNA ASO to the liver and kidney leading to targeted gene knockdown. These data suggest delivery remains a key obstacle to topically administered, naked oligonucleotide efficacy in the lung and introduce inhalation as a potentially viable alternative to injection for antisense administration to the liver and kidneys. PMID:21971426

  1. 'Naked-eye' detection of biologically important anions in aqueous media by colorimetric receptor and its real life applications

    Science.gov (United States)

    Singh, Archana; Trivedi, Darshak R.

    2017-05-01

    A colorimetric receptor R 2-[(2-Hydroxy-naphthalen-1-ylmethylene)-hydrazonomethyl]-quinolin-8-ol has been designed and synthesized with good yield and characterized by the standard spectroscopic techniques such as FT-IR, UV-Visible, 1H NMR, 13C NMR and ESI-MS. The receptor R showed naked-eye detection and spectral change in the presence of F-, AcO- and H2PO4- over other anions. Interestingly, receptor R displaying high selective recognition towards F-, AcO- ion with a drastic color change from pale yellow to red in dry DMSO solvent and orange in mixed solvent DMSO/H2O (9:1, v/v). The behavior of receptor R towards F-, AcO- ion was investigated using UV-Vis and 1H NMR experiment. The detailed 1H NMR experiment result revealed that the receptor R is forming the hydrogen bonding between imine nitrogen and phenolic sbnd OH proton towards anions. The receptor R is able to detect sodium salts of flouride (NaF) and acetate (NaAcO) in aqueous medium and it exhibited dramatic color change from pale yellow to red. The receptor R demonstrated itself to be useful for real life application by detecting flouride and acetate ion in sea-water and commercially available product such as toothpaste, mouthwash and vinegar solution.

  2. Diet composition and feeding ecology of the naked goby Gobiosoma bosc (Gobiidae) from four western Atlantic estuaries.

    Science.gov (United States)

    D'Aguillo, M C; Harold, A S; Darden, T L

    2014-08-01

    The feeding ecology of the small-bodied benthic naked goby Gobiosoma bosc, a western Atlantic species that occurs in estuaries and other inshore habitats from Connecticut to Texas U.S.A., was investigated in a total of four estuaries spanning South Carolina, North Carolina, Maryland and New Jersey. Gut content analysis of 391 individuals revealed that G. bosc is a benthic microcarnivore that feeds primarily on polychaetes, gammarid amphipods and harpacticoid copepods. Diet composition varied with body size, tidal creek within an estuary and geographic region. Analyses of gut fullness suggest that G. bosc is a daytime visual predator and that nest and egg guarding during the reproductive season reduce foraging activity in mature males. Additionally, G. bosc infected with adult digenean parasites of the gut foraged more intensely than uninfected individuals, a relationship that was strongest for reproductively mature males. Regionally, significant variation in dietary breadth was documented and may reflect a foraging response to a decrease in prey diversity moving from estuaries of higher salinity and lower latitude to estuaries of lower salinity and higher latitude. These results contribute to an understanding of the life history of G. bosc and the role played by this common species in estuarine food webs.

  3. Novel salicylic acid-oriented thiourea-type receptors as colorimetric chemosensor: Synthesis, characterizations and selective naked-eye recognition properties

    Science.gov (United States)

    Li, Shaowei; Cao, Xiufang; Chen, Changshui; Ke, Shaoyong

    2012-10-01

    Based on the salicylic acid backbone, three highly sensitive and selective colorimetric chemosensors with an acylthiourea binding unit have been designed, synthesized and characterized. These chemosensors have been utilized for selective recognition of fluoride anions in dry DMSO solution by typical spectroscopic titration techniques. Furthermore, the obtained chemosensors AR1-3 have shown naked-eye sensitivity for detection of biologically important fluoride ion over other anions in solution.

  4. Human endostatin gene transfer, either naked or with liposome, has the same inhibitory effect on growth of mouse liver tumor cells in vivo

    Science.gov (United States)

    Ma, Chun-Hong; Zhang, Yan; Wang, Xiao-Yan; Gao, Li-Fen; Liu, Hua; Guo, Chun; Liu, Su-Xia; Cao, Ying-Lin; Zhang, Li-Ning; Sun, Wen-Sheng

    2004-01-01

    AIM: To explore a safe and efficient strategy of tumor therapy using anti-angiogenetic agents. METHODS: Endostatin gene with a signal sequence of human IgG γ chain was amplified by PCR and cloned into pVAX1 plasmid which was the only vector authorized by FDA in clinical trial to construct a recombinant plasmid named as pVAX-sEN. The recombinant plasmid was detected with Eco I/Kpn I and DNA sequencing. BALB/c mice bearing hepatocarcinoma cell line H22 were treated with naked pVAX-sEN or liposome-DNA complex in which the dose of DNA and the ratio of DNA and liposome were different from each other. To compare the efficiency of gene transfection, expression of endostatin at the treated tumor site was assayed with ELISA. To investigate the effect of pVAX1-sEN on hepatocellular carcinoma, pVAX-sEN either naked or in liposome-DNA complex was injected into BALB/c mice bearing H22, then the diameter of tumors was measured, microvessel density was detected by immunohistochemistry, endostatin expression in vivo was assayed at different time points. RESULTS: DNA sequencing showed the endostatin gene with the signal peptide was correctly cloned. In situ gene expression assay indicated that both the ratio of DNA and liposome and the dose of DNA could affect the gene transfection efficiency. Interestingly, naked pVAX-sEN had a similar in situ endostatin expression to pVAX-sEN with liposome. Animal experiments showed that pVAX-sEN together with pVAX-sEN-liposome complex could efficiently suppress the growth of mouse hepatoma cells. CONCLUSION: Naked endostatin plasmid intratumoral injection can get a similar gene transfection efficiency to liposome-DNA complex when used in situ. PMID:15334690

  5. Human endostatin gene transfer,either naked or with liposome,has the same inhibitory effect on growth of mouse liver tumor cells in vivo

    Institute of Scientific and Technical Information of China (English)

    Chun-Hong Ma; Wen-Sheng Sun; Yan Zhang; Xiao-Yan Wang; Li-Fen Gao; Hua Liu; Chun Guo; Su-Xia Liu; Ying-Lin Cao; Li-Ning Zhang

    2004-01-01

    AIM: To explore a safe and efficient strategy of tumor therapy using anti-angiogenetic agents.METHODS: Endostatin gene with a signal sequence of human IgG γ chain was amplified by PCR and cloned into pVAX1 plasmid which was the only vector authorized by FDA in clinical trial to construct a recombinant plasmid named as pVAX-sEN. The recombinant plasmid was detected with EcoRI/KpnI and DNA sequencing. BALB/c mice bearing hepatocarcinoma cell line H22 were treated with naked pVAX-sEN or liposome-DNA complex in which the dose of DNA and the ratio of DNA and liposome were different from each other. To compare the efficiency of gene transfection, expression of endostatin at the treated tumor site was assayed with ELISA. To investigate the effect of pVAX1-sEN on hepatocellular carcinoma, pVAX-sEN either naked or in liposome-DNA complex was injected into BALB/c mice bearing H22, then the diameter of tumors was measured, microvessel density was detected by immunohistochemistry, endostatin expression in vivo was assayed at different time points.RESULTS: DNA sequencing showed the endostatin gene with the signal peptide was correctly cloned. In situ gene expression assay indicated that both the ratio of DNA and liposome and the dose of DNA could affect the gene transfection efficiency. Interestingly, naked pVAX-sEN had a similar in situ endostatin expression to pVAX-sEN with liposome. Animal experiments showed that pVAX-sEN together with pVAX-sEN-liposome complex could efficiently suppress the growth of mouse hepatoma cells.CONCLUSION: Naked endostatin plasmid intratumoral injection can get a similar gene transfection efficiency to liposome-DNA complex when used in situ.

  6. Synthesis and characterization of novel urea and thiourea substitute cyclotriphosphazene compounds as naked-eye sensors for F$^{-}$ and CN$^{-}$ anions

    OpenAIRE

    ÖZAY, HAVA; Yildirim, Mehmet; ÖZAY, ÖZGÜR

    2015-01-01

    Novel hexakisurea and thiourea cyclotriphosphazene compounds (3-7) were synthesized. The structure of all compounds was identified using FT-IR $^{1}$H, $^{13}$C, and $^{31}$P NMR spectroscopy; MALDI-TOF MS; and elemental analysis. The optical sensor properties for anions of 3-7 were investigated using UV-Vis spectroscopy. It was determined that compounds 6 and 7 are spectrophotometric and naked eye sensors for F$^{-}$ and CN$^{-}$ anions, respectively. Sensor properties of these compounds for...

  7. Spatial variability of soil electrical conductivity under the mole rats (Spalax microphthalmus digging activity at the different scales

    Directory of Open Access Journals (Sweden)

    A. V. Zhukov

    2012-07-01

    Full Text Available The soil mounds emerged owing to the mole rats’ digging activity have been shown to be characterised by less electrical conductivity than surrounded soil. This effect is due to the changes of the mounds bulk’s density and moisture. The effect of the mole rats’ digging activity on the soil electrical conductivity has been found not to be restricted by the geometrical border of the mounds. The mounds are surrounded by 1–1.5 m halo of increased soil electrical conductivity. The halo size is increased with the aging of the mound and with the compacting of their aggregation.

  8. Comparative Efficacy of Bromadiolone, Cholecalciferol and Zinc Phosphide Against Short -Tailed Mole Rat Nesokia indica in Captivity

    OpenAIRE

    PERVEZ, Amjad; AHMAD, Syed M.; Waqar, S; RIZVI, A.

    1998-01-01

    We conducted no-choice and paired choice feeding trials with individually caged Nesokia indica to evaluate the efficacy of Bromadiolone, Cholecalciferol and Zinc phosphide baits. Under no-choice test (1 day and 3 day) male rats consumed less bromadiolone bait. However, sex-wise difference was observed non-significant. Under choice feeding test, difference between bromadiolone bait intake and sex was observed non-significant. Under cholecalciferol bait, treated bait was consumed more than ...

  9. “信用违约互换”裸卖空的风险与管制%Risks and Regulation of“CDS”Naked Short Selling

    Institute of Scientific and Technical Information of China (English)

    王倩; 吴承礼

    2016-01-01

    Naked short selling as a financial derivative instrument may increase the liquidity risk, credit risks, systematic risks, and even trigger crisis. Empirical studies show CDS (credit default swaps) naked short selling makes the Greek sovereign-debt crisis have expected and self-imposed characteristics. In Greek crisis, European Union forbid CDS naked short selling. In Chinese stock market crash, stock futures naked short selling was completely prohibited. They are both necessary measures taken in the hope that it is not already too late. To root out the negative feedback effects in crisis, we need to curb speculation and interrupt self-feedback mechanism. China should discard “market fundamentalism”, learn from EU experience to be cautious about the innovation of financial instruments, curb speculation, prohibit naked short selling, and at the same time be wary of riskless arbitrage of quasi-municipal bond .%金融衍生工具的祼交易会增加市场的流动性风险、信用风险、系统性风险,甚至引发危机。实证研究表明,“信用违约互换(CDS)”祼卖空交易使希腊主权债务危机具有预期自致的特性。无论是主权债务危机中欧盟严禁信用违约互换祼卖空,还是中国股灾中对股指期货祼交易的全面禁止,都是“亡羊补牢”式的必要措施。要消除危机中的负反馈效应,应抑制投机,阻断危机自强化机制。中国应摒弃“市场原教旨主义”,借鉴欧盟经验,谨慎对待金融衍生工具的创新,抑制投机行为,严禁祼卖空交易;同时应警惕对城投债的“无风险套利”。

  10. Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays.

    Science.gov (United States)

    Peng, Yanfen; Gelder, Victor Van; Amaladoss, Anburaj; Patel, Kadamb Haribhai

    2016-10-21

    This report presents two methods for the covalent immobilization of capture antibodies on cellulose filter paper grade No. 1 (medium-flow filter paper) discs and grade No. 113 (fast-flow filter paper) discs. These cellulose paper discs were grafted with amine functional groups through a silane coupling technique before the antibodies were immobilized on them. Periodate oxidation and glutaraldehyde cross-linking methods were used to graft capture antibodies on the cellulose paper discs. In order to ensure the maximum binding capacity of the capture antibodies to their targets after immobilization, the effects of various concentrations of sodium periodate, glutaraldehyde, and capture antibodies on the surface of the paper discs were investigated. The antibodies that were coated on the amine-functionalized cellulose paper discs through a glutaraldehyde cross-linking agent showed enhanced binding activity to the target when compared to the periodate oxidation method. IgG (in mouse reference serum) was used as a reference target in this study to test the application of covalently immobilized antibodies through glutaraldehyde. A new paper-based, enzyme-linked immunosorbent assay (ELISA) was successfully developed and validated for the detection of IgG. This method does not require equipment, and it can detect 100 ng/ml of IgG. The fast-flow filter paper was more sensitive than the medium-flow filter paper. The incubation period of this assay was short and required small sample volumes. This naked-eye, colorimetric immunoassay can be extended to detect other targets that are identified with conventional ELISA.

  11. MicroRNA-195-5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1.

    Science.gov (United States)

    Qu, Qiang; Chu, Xiangdong; Wang, Peng

    2017-03-01

    MiR-195-5p has been shown to play an essential role in human cancer progression. Nevertheless, the biological role of miR-195-5p in osteosarcoma development remains unclear. In this study, real-time PCR was performed to examine the miR-195-5p expression in human osteosarcoma cell lines. CCK-8 assay and Transwell assay were carried out to measure the effect of miR-195-5p on cell proliferation and invasion. Luciferase reporter assay was used to identify the targets of miR-195-5p. The results showed that miR-195-5p was significantly downregulated in osteosarcoma cells. Forced expression of miR-195-5p significantly inhibited cell proliferation, suppressed cell migration and invasion, compared with wild-type and control-transfected osteosarcoma cells. Luciferase reporter assay revealed that miR-195-5p binds to the 3'-untranslated region (UTR) of Naked cuticle homolog 1 (NKD1), indicating that NKD1 was a novel target of miR-195-5p. NKD1 mRNA and protein levels were reduced after overexpression of miR-195-5p. Moreover, silencing of NKD1 significantly inhibited the proliferation and invasion of osteosarcoma cells. Accordingly, our results support a tumor suppressor role of miR-195-5p in osteosarcoma through inhibiting NKD1, and it may be a promising therapeutic target for osteosarcoma. © 2017 International Federation for Cell Biology.

  12. CONSTRAINTS ON THE EMISSION MODEL OF THE 'NAKED-EYE BURST' GRB 080319B

    Energy Technology Data Exchange (ETDEWEB)

    Abdo, A. A.; Abeysekara, A. U.; Linnemann, J. T. [Department of Physics and Astronomy, Michigan State University, 3245 BioMedical Physical Sciences Building, East Lansing, MI 48824 (United States); Allen, B. T.; Chen, C. [Department of Physics and Astronomy, University of California, Irvine, CA 92697 (United States); Aune, T. [Santa Cruz Institute for Particle Physics, University of California, 1156 High Street, Santa Cruz, CA 95064 (United States); Berley, D.; Goodman, J. A. [Department of Physics, University of Maryland, College Park, MD 20742 (United States); Christopher, G. E.; Kolterman, B. E.; Mincer, A. I. [Department of Physics, New York University, 4 Washington Place, New York, NY 10003 (United States); DeYoung, T. [Department of Physics, Pennsylvania State University, University Park, PA 16802 (United States); Dingus, B. L.; Hoffman, C. M. [Group P-23, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 (United States); Ellsworth, R. W. [School of Physics, Astronomy and Computational Sciences, George Mason University, Fairfax, VA 22030 (United States); Gonzalez, M. M. [Instituto de Astronomia, Universidad Nacional Autonoma de Mexico, D.F., Mexico 04510 (Mexico); Granot, J. [Open University of Israel, 1 University Road, POB 808, Ra' anana 43537 (Israel); Hays, E.; McEnery, J. E. [NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States); Huentemeyer, P. H. [Department of Physics, Michigan Technological University, Houghton, MI 49931 (United States); and others

    2012-07-10

    On 2008 March 19, one of the brightest gamma-ray bursts (GRBs) ever recorded was detected by several ground- and space-based instruments spanning the electromagnetic spectrum from radio to gamma rays. With a peak visual magnitude of 5.3, GRB 080319B was dubbed the 'naked-eye' GRB, as an observer under dark skies could have seen the burst without the aid of an instrument. Presented here are results from observations of the prompt phase of GRB 080319B taken with the Milagro TeV observatory. The burst was observed at an elevation angle of 47 Degree-Sign . Analysis of the data is performed using both the standard air shower method and the scaler or single-particle technique, which results in a sensitive energy range that extends from {approx}5 GeV to >20 TeV. These observations provide the only direct constraints on the properties of the high-energy gamma-ray emission from GRB 080319B at these energies. No evidence for emission is found in the Milagro data, and upper limits on the gamma-ray flux above 10 GeV are derived. The limits on emission between {approx}25 and 200 GeV are incompatible with the synchrotron self-Compton model of gamma-ray production and disfavor a corresponding range (2 eV-16 eV) of assumed synchrotron peak energies. This indicates that the optical photons and soft ({approx}650 keV) gamma rays may not be produced by the same electron population.

  13. Infectivity of chimeric human T-cell leukemia virus type I molecular clones assessed by naked DNA inoculation.

    Science.gov (United States)

    Zhao, T M; Robinson, M A; Bowers, F S; Kindt, T J

    1996-06-25

    Two human T-cell leukemia virus type I (HTLV-I) molecular clones, K30p and K34p were derived from HTLV-I-infected rabbit cell lines. K30p and K34p differ by 18 bp with changes in the long terminal repeats (LTRs) as well as in the gag, pol, and rex but not tax or env gene products. Cells transfected with clone K30p were infectious in vitro and injection of the K30p transfectants or naked K30p DNA into rabbits leads to chronic infection. In contrast, K34p did not mediate infection in vitro or in vivo, although the cell line from which it was derived is fully infectious and K34p transfectants produce intact virus particles. To localize differences involved in the ability of the clones to cause infection, six chimeric HTLV-I clones were constructed by shuffling corresponding fragments containing the substitutions in the LTRs, the gag/pol region and the rex region between K30p and K34p. Cells transfected with any of the six chimeras produced virus, but higher levels of virus were produced by cells transfected with those constructs containing the K30p rex region. Virus production was transient except in cells transfected with K30p or with a chimera consisting of the entire protein coding region of K30p flanked by K34p LTRs; only the transfectants showing persistent virus production mediated in vitro infection. In vivo infection in rabbits following intramuscular DNA injection was mediated by K30p as well as by a chimera of K30p containing the K34p rex gene. Comparisons revealed that virus production was greater and appeared earlier in rabbits injected with K30p. These data suggest that several defects in the K34p clone preclude infectivity and furthermore, provide systems to explore functions of HTLV-I genes.

  14. Effect of particle size on solubility, dissolution rate, and oral bioavailability: evaluation using coenzyme Q10 as naked nanocrystals

    Directory of Open Access Journals (Sweden)

    Sun J

    2012-11-01

    Full Text Available Jiao Sun,1 Fan Wang,1,2 Yue Sui,1 Zhennan She,1 Wenjun Zhai,1 Chunling Wang,1 Yihui Deng11College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China; 2Beijing Zhijianjinrui Applied Pharmaceutical Science Inc, Beijing, ChinaAbstract: In this paper work, four naked nanocrystals (size range 80–700 nm were prepared without any surfactant or polymer using the solvent/nonsolvent method. The effects of particle size on their solubility, dissolution, and oral bioavailability were investigated. Solubility and dissolution testing were performed in three types of dissolution medium, and the studies demonstrated that the equilibrium solubilities of coenzyme Q10 nanocrystals and bulk drugs were not affected by the dissolution media but the kinetic solubilities were. Kinetic solubility curves and changes in particle size distribution were determined and well explained by the proposed solubilization model for the nanocrystals and bulk drugs. The particle size effect on dissolution was clearly influenced by the diffusion coefficients of the various dissolution media, and the dissolution velocity of coenzyme Q10 increased as particle size decreased. The bioavailability of coenzyme Q10 after oral administration in beagle dogs was improved by reducing the particle size. For 700 nm nanocrystals, the AUC0–48 was 4.4-fold greater than that for the coarse suspensions, but a further decrease in particle size from 700 nm to 120 nm did not contribute to improvement in bioavailability until the particle size was reduced to 80 nm, when bioavailability was increased by 7.3-fold.Keywords: particle size, solubility, dissolution, nanocrystal, bioavailability, coenzyme Q10

  15. Microbial Populations in Naked Neck Chicken Ceca Raised on Pasture Flock Fed with Commercial Yeast Cell Wall Prebiotics via an Illumina MiSeq Platform.

    Directory of Open Access Journals (Sweden)

    Si Hong Park

    Full Text Available Prebiotics are non-digestible carbohydrate dietary supplements that selectively stimulate the growth of one or more beneficial bacteria in the gastrointestinal tract of the host. These bacteria can inhibit colonization of pathogenic bacteria by producing antimicrobial substances such as short chain fatty acids (SCFAs and competing for niches with pathogens within the gut. Pasture flock chickens are generally raised outdoors with fresh grass, sunlight and air, which represents different environmental growth conditions compared to conventionally raised chickens. The purpose of this study was to evaluate the difference in microbial populations from naked neck chicken ceca fed with commercial prebiotics derived from brewer's yeast cell wall via an Illumina MiSeq platform. A total of 147 day-of-hatch naked neck chickens were distributed into 3 groups consisted of 1 C: control (no prebiotic, 2 T1: Biolex® MB40 with 0.2%, and 3 T2: Leiber® ExCel with 0.2%, consistently supplemented prebiotics during the experimental period. At 8 weeks, a total of 15 birds from each group were randomly selected and ceca removed for DNA extraction. The Illumina Miseq platform based on V4 region of 16S rRNA gene was applied for microbiome analysis. Both treatments exhibited limited impact on the microbial populations at the phylum level, with no significant differences in the OTU number of Bacteroidetes among groups and an increase of Proteobacteria OTUs for the T1 (Biolex® MB40 group. In addition there was a significant increase of genus Faecalibacterium OTU, phylum Firmicutes. According to the development of next generation sequencing (NGS, microbiome analysis based on 16S rRNA gene proved to be informative on the prebiotic impact on poultry gut microbiota in pasture-raised naked neck birds.

  16. The Naked Blunder

    CERN Multimedia

    Computer Security Team

    2013-01-01

    In the past, we have published many different articles on a multitude of aspects of computer security and on the risk to CERN and to you when you use your computer at CERN or at home. Some were relevant to all of us, others only to a minority. This time, we would like to raise a rather delicate and fortunately rare issue: the consultation of pornographic websites and why it is forbidden at CERN.   With 10,000 people at CERN of different cultural and professional backgrounds, some working outside traditional working hours and some staying in one of the three CERN hostels, it is a natural fact that behaviours, interests, perceptions and attitudes vary significantly. CERN highly values the diversity of its personnel and users and the interaction between them. In this respect, the CERN campus is comparable to a small city and, as in every small city, some citizens are attracted to pornography. It is not easy to find a common definition of pornography. It depends on our cultural roots, our value...

  17. The Naked Manager

    DEFF Research Database (Denmark)

    Meier Sørensen, Bent; Villadsen, Kaspar

    2015-01-01

    This article explores how an allegedly ‘non-hierarchical’ and aestheticized managerial practice reconfigures power relations within a creative industry. The key problematic is ‘governmental’ in the sense suggested by Michel Foucault, in as much as the manager’s ethical self-practice—which involves...... their apparently eccentric Managing Director, Peter Aalbæk. Contrary to much of the literature discussing embodiment and ethics in organization studies, we do not identify an ‘ethics of organization’ dominated by instrumental rationality, efficiency and desire for profit which is ostensibly juxtaposed to a non......-alienating, embodied ethics. Rather, when the body becomes invested in management, we observe tensions, tactics of domination and unpredictability....

  18. Silencing the Honey Bee (Apis mellifera) Naked Cuticle Gene (nkd) Improves Host Immune Function and Reduces Nosema ceranae Infections.

    Science.gov (United States)

    Li, Wenfeng; Evans, Jay D; Huang, Qiang; Rodríguez-García, Cristina; Liu, Jie; Hamilton, Michele; Grozinger, Christina M; Webster, Thomas C; Su, Songkun; Chen, Yan Ping

    2016-11-15

    Nosema ceranae is a new and emerging microsporidian parasite of European honey bees, Apis mellifera, that has been implicated in colony losses worldwide. RNA interference (RNAi), a posttranscriptional gene silencing mechanism, has emerged as a potent and specific strategy for controlling infections of parasites and pathogens in honey bees. While previous studies have focused on the silencing of parasite/pathogen virulence factors, we explore here the possibility of silencing a host factor as a mechanism for reducing parasite load. Specifically, we used an RNAi strategy to reduce the expression of a honey bee gene, naked cuticle (nkd), which is a negative regulator of host immune function. Our studies found that nkd mRNA levels in adult bees were upregulated by N. ceranae infection (and thus, the parasite may use this mechanism to suppress host immune function) and that ingestion of double-stranded RNA (dsRNA) specific to nkd efficiently silenced its expression. Furthermore, we found that RNAi-mediated knockdown of nkd transcripts in Nosema-infected bees resulted in upregulation of the expression of several immune genes (Abaecin, Apidaecin, Defensin-1, and PGRP-S2), reduction of Nosema spore loads, and extension of honey bee life span. The results of our studies clearly indicate that silencing the host nkd gene can activate honey bee immune responses, suppress the reproduction of N. ceranae, and improve the overall health of honey bees. This study represents a novel host-derived therapeutic for honey bee disease treatment that merits further exploration. Given the critical role of honey bees in the pollination of agricultural crops, it is urgent to develop strategies to prevent the colony decline induced by the infection of parasites/pathogens. Targeting parasites and pathogens directly by RNAi has been proven to be useful for controlling infections in honey bees, but little is known about the disease impacts of RNAi silencing of host factors. Here, we demonstrate

  19. Study on Preparation Technology of Naked Oats Beverage Fermented by Lactic Acid Bacteria%乳酸发酵型莜麦饮料的制备工艺研究

    Institute of Scientific and Technical Information of China (English)

    王健; 刘媛; 兰凤英

    2012-01-01

    [ Objective ] The aim was to prepare a lactic acid fermentation beverage of naked oats. [ Method ] With saccharified naked oats juice as raw material, lactic acid bacteria as fermented strain, effects of strain domestication, inoculation amount and fermentation time on the beverage quality were studied. [ Result] The preparation technology of lactic acid fermentation beverage of naked oats was as follows:with saccharified naked oats juice as raw material, domesticated lactic acid bacteria as fermented strain, inoculation amount 4% ,then fermented 6 h at the temperature of 42 ℃. [Conclusion] The study provided a scientific basis for development of naked oats foods.%[目的]制备一种乳酸发酵型莜麦饮料.[方法]以糖化后的莜麦汁为原料、乳酸茵为发酵菌种,探讨菌种驯化、接种量和发酵时间对乳酸发酵型莜麦饮料品质的影响.[结果]乳酸发酵型莜麦饮料的制备工艺为以糖化后的莜麦汁为原料,采用驯化后的乳酸茵为发酵菌种,接种量4%,在42℃下发酵6h.[结论]该研究为莜麦食品的开发提供了科学依据.

  20. An efficient probe for rapid detection of cyanide in water at parts per billion levels and naked-eye detection of endogenous cyanide.

    Science.gov (United States)

    Kumari, Namita; Jha, Satadru; Bhattacharya, Santanu

    2014-03-01

    A new molecular probe based on an oxidized bis-indolyl skeleton has been developed for rapid and sensitive visual detection of cyanide ions in water and also for the detection of endogenously bound cyanide. The probe allows the "naked-eye" detection of cyanide ions in water with a visual color change from red to yellow (Δλmax =80 nm) with the immediate addition of the probe. It shows high selectivity towards the cyanide ion without any interference from other anions. The detection of cyanide by the probe is ratiometric, thus making the detection quantitative. A Michael-type addition reaction of the probe with the cyanide ion takes place during this chemodosimetric process. In water, the detection limit was found to be at the parts per million level, which improved drastically when a neutral micellar medium was employed, and it showed a parts-per-billion-level detection, which is even 25-fold lower than the permitted limits of cyanide in water. The probe could also efficiently detect the endogenously bound cyanide in cassava (a staple food) with a clear visual color change without requiring any sample pretreatment and/or any special reaction conditions such as pH or temperature. Thus the probe could serve as a practical naked-eye probe for "in-field" experiments without requiring any sophisticated instruments. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  1. Naked eye and spectrophotometric detection of chromogenic insecticide in aquaculture using amine functionalized gold nanoparticles in the presence of major interferents

    Science.gov (United States)

    Loganathan, C.; John, S. Abraham

    2017-02-01

    Detection of a chromogenic insecticide, malachite green (MG) using 3,5-diamino-1,2,4-triazole capped gold nanoparticles (DAT-AuNPs) by both naked eye and spectrophotometry was described in this paper. The DAT-AuNPs were prepared by wet chemical method and show absorption maximum at 518 nm. The zeta potential of DAT-AuNPs was found to be - 39.9 mV, suggesting that one of the amine groups of DAT adsorbed on the surface of AuNPs and the other amine group stabilizes the AuNPs from aggregation. The wine red color DAT-AuNPs changes to violet while adding 25 μM MG whereas the absorption band at 518 nm was increased and shifted towards longer wavelength. However, addition of 70 μM MG leads to the aggregation of DAT-AuNPs. This is due to strong electrostatic interaction between ammonium ion of MG and the free amine group of DAT. Based on the color change and shift in SPR band, 25 and 5 μM MG can be easily detected by naked eye and spectrophotometry. The DAT-AuNPs show high selectivity towards MG even in the presence of 5000-fold higher concentrations of common interferents. The practical application was successfully demonstrated by determining MG in fish farm water.

  2. The effect of different agrotechnical levels on weed infestation in crops of naked and husked varieties of oat (Avena sativa L.

    Directory of Open Access Journals (Sweden)

    Sylwia Andruszczak

    2012-12-01

    Full Text Available The present study was carried out in the period 2007- 2009 in the Experimental Farm in Bezek near the city of Chełm. The aim of the investigation was to compare weed infestation of the husked (Krezus and naked (Cacko oat cultivars cultivated under conditions of different weed control treatments. The following weed control levels were compared: A - control object; B - harrowing twice; C - application of the herbicide Mustang 306 SE; D - herbicide Mustang 306 SE + foliar fertilizer Insol 3; E - herbicide Mustang 306 SE + two foliar fertilizers: Insol 3 and FoliCare 18:18:18. The husked oat cv. Krezus was more competitive against weeds. Number of dicotyledonous weeds and of monocotyledonous weeds, total weeds, and air-dry weight of above-ground parts of weeds in the crop of this cultivar were all significantly lower compared to the naked cv. Cacko. From the group of dicotyledonous species, Chenopodium album, Galinsoga parviflora, Matricaria maritima ssp. inodora, Polygonum aviculare, and Stellaria media were the dominant species in the crops of the oat varieties under investigation, whereas among the monocotyledonous species Echinochloa crus-galli, Setaria pumila, Apera spica-venti, and Elymus repens occurred in greatest numbers. The application of the herbicide Mustang 306 SE decreased the number of dicotyledonous weeds and air-dry weight of weeds, compared to the treatment in which mechanical weed control had been used.

  3. Naked eye and spectrophotometric detection of chromogenic insecticide in aquaculture using amine functionalized gold nanoparticles in the presence of major interferents.

    Science.gov (United States)

    Loganathan, C; John, S Abraham

    2017-02-15

    Detection of a chromogenic insecticide, malachite green (MG) using 3,5-diamino-1,2,4-triazole capped gold nanoparticles (DAT-AuNPs) by both naked eye and spectrophotometry was described in this paper. The DAT-AuNPs were prepared by wet chemical method and show absorption maximum at 518nm. The zeta potential of DAT-AuNPs was found to be -39.9mV, suggesting that one of the amine groups of DAT adsorbed on the surface of AuNPs and the other amine group stabilizes the AuNPs from aggregation. The wine red color DAT-AuNPs changes to violet while adding 25μM MG whereas the absorption band at 518nm was increased and shifted towards longer wavelength. However, addition of 70μM MG leads to the aggregation of DAT-AuNPs. This is due to strong electrostatic interaction between ammonium ion of MG and the free amine group of DAT. Based on the color change and shift in SPR band, 25 and 5μM MG can be easily detected by naked eye and spectrophotometry. The DAT-AuNPs show high selectivity towards MG even in the presence of 5000-fold higher concentrations of common interferents. The practical application was successfully demonstrated by determining MG in fish farm water.

  4. The first report of luminescent liver tissue in fishes: evolution and structure of bioluminescent organs in the deep-sea naked barracudinas (Aulopiformes: Lestidiidae).

    Science.gov (United States)

    Ghedotti, Michael J; Barton, Ryan W; Simons, Andrew M; Davis, Matthew P

    2015-03-01

    Bioluminescent organs that provide ventral camouflage are common among fishes in the meso-bathypelagic zones of the deep sea. However, the anatomical structures that have been modified to produce light vary substantially among different groups of fishes. Although the anatomical structure and evolutionary derivation of some of these organs have been well studied, the light organs of the naked barracudinas have received little scientific attention. This study describes the anatomy and evolution of bioluminescent organs in the Lestidiidae (naked barracudinas) in the context of a new phylogeny of barracudinas and closely related alepisauroid fishes. Gross and histological examination of bioluminescent organs or homologous structures from preserved museum specimens indicate that the ventral light organ is derived from hepatopancreatic tissue and that the antorbital spot in Lestrolepis is, in fact, a second dermal light organ. In the context of the phylogeny generated from DNA-sequence data from eight gene fragments (7 nuclear and 1 mitochondrial), a complex liver with a narrow ventral strand running along the ventral midline evolves first in the Lestidiidae. The ventral hepatopancreatic tissue later evolves into a ventral bioluminescent organ in the ancestor of Lestidium and Lestrolepis with the lineage leading to the genus Lestrolepis evolving a dermal antorbital bioluminescent organ, likely for light-intensity matching. This is the first described hepatopancreatic bioluminescent organ in fishes.

  5. In vivo Site-Specific Transfection of Naked Plasmid DNA and siRNAs in Mice by Using a Tissue Suction Device

    Science.gov (United States)

    Shimizu, Kazunori; Kawakami, Shigeru; Hayashi, Kouji; Kinoshita, Hideyuki; Kuwahara, Koichiro; Nakao, Kazuwa; Hashida, Mitsuru; Konishi, Satoshi

    2012-01-01

    We have developed an in vivo transfection method for naked plasmid DNA (pDNA) and siRNA in mice by using a tissue suction device. The target tissue was suctioned by a device made of polydimethylsiloxane (PDMS) following the intravenous injection of naked pDNA or siRNA. Transfection of pDNA encoding luciferase was achieved by the suction of the kidney, liver, spleen, and heart, but not the duodenum, skeletal muscle, or stomach. Luciferase expression was specifically observed at the suctioned region of the tissue, and the highest luciferase expression was detected at the surface of the tissue (0.12±0.03 ng/mg protein in mice liver). Luciferase expression levels in the whole liver increased linearly with an increase in the number of times the liver was suctioned. Transfection of siRNA targeting glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene significantly suppressed the expression of GAPDH mRNA in the liver. Histological analysis shows that severe damage was not observed in the suctioned livers. Since the suction device can be mounted onto the head of the endoscope, this method is a minimally invasive. These results indicate that the in vivo transfection method developed in this study will be a viable approach for biological research and therapies using nucleic acids. PMID:22844458

  6. 糖化莜麦汁最佳发酵菌种的筛选%Screening of the Best Strain for Fermentation of Naked Oat Juice after Saccharification

    Institute of Scientific and Technical Information of China (English)

    王健; 刘媛; 兰凤英

    2013-01-01

      以优质莜麦为原料,对其进行磨粉、糖化等处理后,分别接种经不同处理的即发活性干酵母、混合乳酸菌和葡萄酒活性干酵母3种菌种进行发酵,根据发酵莜麦汁的理化指标和感官评分,从中筛选发酵糖化莜麦汁的适宜菌种。结果表明:葡萄酒活性干酵母的发酵效果最好,其次为驯化混合乳酸菌。%The top quality of naked oats by milling and saccharification was used as the main raw material, which was then fermented with three strains (instant active dry yeast, Lactic Acid Bacteria and wine active dry yeast ) by different treated. The best strain was screened out by the physicochemical index and sense scores of fermentive naked oat juice. The result indicated that the best strains was wine active dry yeast, and secondly was tamed lactic acidbacteria.

  7. ROLE OF COLPOSCOPY USING MODIFIED REID’S INDEX IN SCREENING OF CERVICAL CANCER IN WOMEN WITH ABNORMAL CERVIX ON NAKED EYE EXAMINATION

    Directory of Open Access Journals (Sweden)

    Deshpande

    2014-01-01

    Full Text Available OBJECTIVE: To assess the validity of Colposcopy using Modified Reid's Index as a screening tool in cervical cancer in women with abnormal cervix on naked eye examination . METHODS: This observational study was carried out in Government medical college, Aurangabad from June 2011 to May 2013 . Total 392 women with abnormal cervix on naked eye examination of underwent colposcopy and diagnosis was made on the basis of Modified R eid’s Index. Colposcope directed biopsies were obtained from the abnormal areas. In women with normal colposcopic findings four quadrant biopsies from squamo - columnar junction were taken .Eight women were excluded from analysis owing to unsatisfactory colp oscopy. Results of colposcopy were validated by calculating sensitivity, specificity, positive predictive value, negative predictive value in diagnosing histopathologically confirmed lesions which served as gold standard. RESULTS: Overall sensitivity of colposcopy in all grades of lesions is around 90% or more, touching 100% in high order of histological lesions.Overall specificity is also high.It does have an excellent negative predictive value but comparatively lower positive predictive value especially in high order lesions with fair degree of accuracy in all grades of lesions in the hands of an experienced operator. CONCLUSION: Colposcopy using Modified Reid’s Index with high sensitivity and specificity is a good screening tool for cervical cancer

  8. Large-area, uniform and low-cost dual-mode plasmonic naked-eye colorimetry and SERS sensor with handheld Raman spectrometer.

    Science.gov (United States)

    Xu, Zhida; Jiang, Jing; Wang, Xinhao; Han, Kevin; Ameen, Abid; Khan, Ibrahim; Chang, Te-Wei; Liu, Gang Logan

    2016-03-21

    We demonstrated a highly-sensitive, wafer-scale, highly-uniform plasmonic nano-mushroom substrate based on plastic for naked-eye plasmonic colorimetry and surface-enhanced Raman spectroscopy (SERS). We gave it the name FlexBrite. The dual-mode functionality of FlexBrite allows for label-free qualitative analysis by SERS with an enhancement factor (EF) of 10(8) and label-free quantitative analysis by naked-eye colorimetry with a sensitivity of 611 nm RIU(-1). The SERS EF of FlexBrite in the wet state was found to be 4.81 × 10(8), 7 times stronger than in the dry state, making FlexBrite suitable for aqueous environments such as microfluid systems. The label-free detection of biotin-streptavidin interaction by both SERS and colorimetry was demonstrated with FlexBrite. The detection of trace amounts of the narcotic drug methamphetamine in drinking water by SERS was implemented with a handheld Raman spectrometer and FlexBrite. This plastic-based dual-mode nano-mushroom substrate has the potential to be used as a sensing platform for easy and fast analysis in chemical and biological assays.

  9. Identification of a small, naked virus in tumor-like aggregates in cell lines derived from a green turtle, Chelonia mydas, with fibropapillomas

    Science.gov (United States)

    Lu, Y.; Aguirre, A.A.; Work, T.M.; Balazs, G.H.; Nerurkar, V.R.; Yanagihara, R.

    2000-01-01

    Serial cultivation of cell lines derived from lung, testis, periorbital and tumor tissues of a green turtle (Chelonia mydas) with fibropapillomas resulted in the in vitro formation of tumor-like cell aggregates, ranging in size from 0.5 to 2.0 mm in diameter. Successful induction of tumor-like aggregates was achieved in a cell line derived from lung tissue of healthy green turtles, following inoculation with cell-free media from these tumor-bearing cell lines, suggesting the presence of a transmissible agent. Thin-section electron microscopy of the cell aggregates revealed massive collagen deposits and intranuclear naked viral particles, measuring 50??5 nm in diameter. These findings, together with the morphological similarity between these tumor-like cell aggregates and the naturally occurring tumor, suggest a possible association between this novel virus and the disease. Further characterization of this small naked virus will clarify its role in etiology of green turtle fibropapilloma, a life-threatening disease of this endangered marine species. Copyright (C) 2000 Elsevier Science B.V.

  10. Resistência ao Estresse Calórico em Frangos de Corte de Pescoço Pelado Resistance to Heat Stress of Naked Neck Broilers

    Directory of Open Access Journals (Sweden)

    MAN Silva

    2001-01-01

    Full Text Available Aves de duas linhagens, sendo uma portadora do gene pescoço pelado (Na_ que determina redução no empenamento, e outra não portadora, com empenamento normal (nana, foram submetidas a estresse térmico gradativo (38, 40 e 42ºC, em câmara climática, nas idades de 28, 35 e 42 dias, com o intuito de se verificar a resistência ao estresse térmico. Foram verificadas diferenças significativas entre a temperatura retal média e a taxa respiratória média da linhagem de empenamento normal quando comparada com a linhagem de pescoço pelado, em todas as idades e períodos de estresse. Foram verificados valores mais altos das aves de empenamento normal, demonstrando a influência do conjunto temperatura de estresse térmico e idade. Pôde-se constatar que houve diferença significativa entre as linhagens para as médias de perda de peso apenas aos 35 dias de idade. Os resultados obtidos sugerem que a linhagem de pescoço pelado (Na_ possui maior resistência ao estresse térmico em relação à linhagem de empenamento normal (nana.Birds of two lineages, a carrier of the Naked Neck gene (Na_ that determines reduction in the feathering and other non-carrier (normal feathering, nana, were submitted to thermal stress (38, 40 and 42ºC in climatic chambers at 28, 35 and 42 days of age to verify the resistance to the thermal stress. Significant differences were verified among the mean rectal temperature of the normal feathered lineage when compared with the naked neck lineage, in all the ages and stress periods, with higher values in the normal feathered birds. The same result was found for mean respiratory rate. Significant difference was found for weight loss between the two lineages only at the age of 35 days. The results obtained suggest that the Naked Neck lineage possesses higher resistance to the thermal stress in relation to the normal feathered lineage.

  11. Naked Eye 3D Technology of Full Color LED Display Screen%浅谈全彩LED显示屏的裸眼3D技术

    Institute of Scientific and Technical Information of China (English)

    马小华

    2014-01-01

    This paper introduces the 3D stereo vision principle and 3 Naked-eye 3D display technology is applied to the principle of LED display screen and application method, several 3D autostereoscopic display quality technology application in the display of the LED potential analysis and research and discussion.%本文介绍了3D立体视觉原理以及三种裸眼3D显示技术应用到LED显示屏上的原理以及应用方法,对几种裸眼3D立体显示技术应用在LED显示屏上的优劣势进行分析和研究探讨。

  12. Discussion on the Origin and Taxonomy of Naked Oat( Avena nuda L.)%大粒裸燕麦(莜麦)(Avena nuda L.)起源及分类问题的探讨

    Institute of Scientific and Technical Information of China (English)

    郑殿升; 张宗文

    2011-01-01

    There are five cultivated species in the genus Avena L. They are common oat (A. Sativa L. ) , Ethiopian (Abyssinian) oat (A. Abyssinica Hochst. ) , Byzant( Red) oat( A. Byzantina Koch. ) , Hispid ( Small) oat ( A. Strigosa Schreb. ) ,and naked oat(A. Nuda L. ). Naked oat is characterized by its hulless seeds,while other oats by their hulled seeds. Naked oat is mainly planted in China and hulled oats are grown in other countries. Therefore, naked oat is important among oats in the world. However, the viewpoints on the origin and taxonomy of naked oat were disputable. The authors believe that the origin of naked oat would be the adjacent region of Shanxi and Inner Mongolia,and the taxonomic status of naked oat would be an independent species,I. E. ,A. Nuda L.%燕麦属(Avena L.)植物中有5个栽培种即普通栽培燕麦(A.sativa L.)、埃塞俄比亚燕麦(A.abyssinica Hochst.)、地中海燕麦(A.byzantina Koch)、砂燕麦(A.strigosa Schreb.)和大粒裸燕麦又称莜麦(A.nudaL.),其中大粒裸燕麦的子粒不带稃皮为裸燕麦,其他物种均带稃皮为皮燕麦.国际上主要种植皮燕麦,而我国主要种植大粒裸燕麦,由此不难看出,大粒裸燕麦在世界燕麦中占有特殊的地位.然而,关于大粒裸燕麦的起源和分类地位问题,迄今学者们的意见仍不尽相同.本文通过参阅有关文献和研究实践,对这两个问题进行探讨,认为大粒裸燕麦起源于我国山西和内蒙古一带,在植物学分类上应为一个独立的物种即A.nuda L..

  13. Large-area, uniform and low-cost dual-mode plasmonic naked-eye colorimetry and SERS sensor with handheld Raman spectrometer

    CERN Document Server

    Xu, Zhida; Wang, Xinhao; Han, Kevin; Ameen, Abid; Khan, Ibrahim; Chang, Te-Wei; Liu, Gang Logan

    2016-01-01

    We demonstrated a highly sensitive, waferscale, highly uniform plasmonic nanomushroom substrate based on plastic for nakedeye plasmonic colorimetry and surface enhanced Raman spectroscopy (SERS). We gave it the name FlexBrite. The dualmode functionality of FlexBrite allows for label-free qualitative analysis by SERS with an enhancement factor (EF) of 10^8 and labelfree quantitative analysis by naked eye colorimetry with a sensitivity of 611 nm RIU-1. The SERS EF of FlexBrite in the wet state was found to be 4.81 X 10^8, 7 times stronger than in the dry state, making FlexBrite suitable for aqueous environments such as microfluid systems. The labelfree detection of biotin streptavidin interaction by both SERS and colorimetry was demonstrated with FlexBrite. The detection of trace amounts of the narcotic drug methamphetamine in drinking water by SERS was implemented with a handheld Raman spectrometer and FlexBrite. This plastic based dual-mode nano-mushroom substrate has the potential to be used as a sensing pla...

  14. Naked Polyamidoamine Polymers Intrinsically Inhibit Angiotensin II-Mediated EGFR and ErbB2 Transactivation in a Dendrimer Generation- and Surface Chemistry-Dependent Manner.

    Science.gov (United States)

    Akhtar, Saghir; El-Hashim, Ahmed Z; Chandrasekhar, Bindu; Attur, Sreeja; Benter, Ibrahim F

    2016-05-01

    The effects of naked polyamidoamine (PAMAM) dendrimers on renin-angiotensin system (RAS) signaling via Angiotensin (Ang) II-mediated transactivation of the epidermal growth factor receptor (EGFR) and the closely related family member ErbB2 (HER2) were investigated. In primary aortic vascular smooth muscle cells, a cationic fifth-generation (G5) PAMAM dendrimer dose- and time-dependently inhibited Ang II/AT1 receptor-mediated transactivation of EGFR and ErbB2 as well as their downstream signaling via extracellular-regulated kinase 1/2 (ERK1/2). Inhibition even occurred at noncytotoxic concentrations at short (1 h) exposure times and was dependent on dendrimer generation (G7 > G6 > G5 > G4) and surface group chemistry (amino > carboxyl > hydroxyl). Mechanistically, the cationic G5 PAMAM dendrimer inhibited Ang II-mediated transactivation of EGFR and ErbB2 via inhibition of the nonreceptor tyrosine kinase Src. This novel, early onset, intrinsic biological action of PAMAM dendrimers as inhibitors of the Ang II/AT1/Src/EGFR-ErbB2/ERK1/2 signaling pathway could have important toxicological and pharmacological implications.

  15. High levels of gene expression in the hepatocytes of adult mice, neonatal mice and tree shrews via retro-orbital sinus hydrodynamic injections of naked plasmid DNA.

    Science.gov (United States)

    Yan, Shaoduo; Fu, Qiuxia; Zhou, Yong; Wang, Jidong; Liu, Ying; Duan, Xiangguo; Jia, Shuaizheng; Peng, Jianchun; Gao, Bo; Du, Juan; Zhou, Qianqian; Li, Yuan; Wang, Xiaohui; Zhan, Linsheng

    2012-08-10

    Hydrodynamic-based gene delivery has emerged as an efficient and simple method for the intracellular transfection of naked plasmid DNA (pDNA) in vivo. In this system, a hydrodynamic injection via the tail vein is the most effective non-viral method of liver-targeted gene delivery. However, this injection is often technically challenging when used in animals whose tail veins are difficult to visualize or too small to operate on. To overcome this limitation, an alternative in vivo gene delivery method, the rapid injection of large volume of pDNA solution through retro-orbital sinus, was established. Using this technique, we successfully delivered pDNA to the tissue of adult mice, neonatal mice and tree shrews. The efficient expression of exogenous genes was specifically detected in the liver of test animals treated with this gene delivery method. This study demonstrates for the first time that the hydrodynamic gene delivery via the retro-orbital sinus can not only reach the same transgene efficiency as a tradition hydrodynamic-based intravascular injection but also be used in animals that are difficult to inject via the tail vein. This method could open up new areas in gene function studies and gene therapy disease treatment.

  16. Literary Film Adaptation for Screen Production: the Analysis of Style Adaptation in the Film Naked Lunch from a Quantitative and Descriptive Perspective

    Directory of Open Access Journals (Sweden)

    Alejandro

    2015-11-01

    Full Text Available The study of film adaptations, particularly those coming from literature, has been growing at a rapid rate during the last years due to the amount of adaptations coming from both mainstream and independent film industries. The focus of these studies though is generally addressed to best sellers where the literary style is clearly adaptable to the screen; however, there are cases where the adaptive process has resulted in an entirely different outcome. Naked Lunch, written by William Burroughs and adapted to screen by David Cronenberg, represents a bold change if compared with other novels turned into films. The purpose of this article is to descriptively analyse the literary style in order to understand how it has been represented and adapted into Cronenberg’s film. The analysis will be performed helped with computer-based tools to support statements, using the novel itself as corpus and adding theoretical ideas from Leech and Short’s perspectives. The results suggest that the author’s corrosive, sexually-fuelled claustrophobic style has been translated following three patterns in terms of adaptation theory, although Burroughs, in the hands of Cronenberg, becomes an almost new classification of adaptation, not only adding literary features to the film, but also distinguishable core moments of the writer’s life to the final product. The analysis conducted encourages the study of other uncommon adaptations from literary authors in order to understand the adaptive process followed by filmmakers.

  17. Test of New Introduced Pumpkin Cultivars for Naked Kernel Seed-used in Henan Area%河南地区露地裸仁籽用南瓜新品种引进试验

    Institute of Scientific and Technical Information of China (English)

    徐小军; 张桂兰

    2015-01-01

    为促进河南地区裸仁籽用南瓜产业发展,2013-2014年中国农业科学院郑州果树研究所从甘肃武威金苹果公司引进3个裸仁籽用南瓜品种,并在河南地区进行种植试验。试验结果表明,金苹果三星裸仁籽用南瓜品种商品性状最好,最具推广价值。%In order to promote the industry development of naked kernel seed-used pumpkin in Henan area, we intro-duced three naked kernel seed-used pumpkin cultivars from the Jinpingguo company of Wuwei city in the period of 2013 to 2014, and carried out planting test in Henan province. The results showed that, the cultivar Jinpingguosanxing had good commercial characters, which owned promotional value. Meanwhile, we mastered cultivation techniques of naked kernel seed-used pumpkin in Henan area.

  18. A seven-year storage report of good manufacturing practice-grade naked plasmid DNA: stability, topology, and in vitro/in vivo functional analysis.

    Science.gov (United States)

    Walther, Wolfgang; Schmeer, Marco; Kobelt, Dennis; Baier, Ruth; Harder, Alexander; Walhorn, Volker; Anselmetti, Dario; Aumann, Jutta; Fichtner, Iduna; Schleef, Martin

    2013-12-01

    The great interest for naked plasmid DNA in gene therapy studies is reflected by the fact that it is currently used in 18% of all gene therapy trials. Therefore, validation of topology and functionality of DNA resulting from its long-term stability is an essential requirement for safe and effective gene transfer. To this aim, we analyzed the stability of good manufacturing practice-grade pCMVβ reporter plasmid DNA by capillary gel electrophoresis, agarose gel electrophoresis, and atomic force microscopy. The plasmid DNA was produced for a clinical gene transfer study started in 2005 and was stored for meanwhile 7 years under continuously monitored conditions at -20 °C. The stability of plasmid DNA was monitored by LacZ transgene expression functional assays performed in vitro and in vivo on the 7-year-old plasmid DNA samples compared with plasmid batches newly produced in similar experimental conditions and quality standards. The analyses revealed that during the overall storage time and conditions, the proportion of open circular and supercoiled or covalently closed circular forms is conserved without linearization or degradation of the plasmid. The in vitro transfection and the in vivo jet-injection of DNA showed unaltered functionality of the long-stored plasmid. In summary, the 7-year-old and the newly produced plasmid samples showed similar topology and expression performance. Therefore, our stable storage conditions are effective to preserve the integrity of the DNA to be used in clinical studies. This is an important prerequisite for the long-term performance of gene transfer materials used in trials of long duration as well as of the reference material used in standardization procedures and assays.

  19. Study on the technology of naked oat cookies%莜麦曲奇饼干加工工艺研究

    Institute of Scientific and Technical Information of China (English)

    莎娜; 王国泽; 游新勇; 宋任平

    2014-01-01

    以莜麦粉、奶粉、白砂糖为原料,通过对最佳原料配方和工艺参数的研究,制作一种新型莜麦曲奇饼干。结果表明:莜麦曲奇饼干的加工工艺条件为莜麦粉50 g,黄油105 g,白砂糖75 g,奶粉10 g,搅拌时间12 min,烘烤时间33 min,上火温度180℃,下火温度170℃。在此工艺下制作的莜麦曲奇饼干外观和内在质量都较好,风味能被消费者所接受,并且营养价值高于普通面包。%The experiment with nakedoat flour,dried milk,sugar as raw materials,produced a kind of nakedoatscookies,through researchof theoptimal ingredients and processing technology.The test results showed that the processconditionsof nakedoatscookies:nakedoat flour 50 g,butter 105 g, sugar 75 g,dried milk 10g,stirring time 12 min,baking time 33 min,surface fire temperature 180℃, bottom fire temperature 170℃. In these processes,the appearance and intrinsic qualityof the naked oatscookies were better,theoverall qualitycan be accepted byconsumers,and its nutritional value was more thanordinarycookies.

  20. A Genetic Linkage Map for Naked Oat (Avena nuda L.)%大粒裸燕麦(Avena nuda L.)遗传连锁图谱的构建

    Institute of Scientific and Technical Information of China (English)

    徐微; 张宗文; 张恩来; 吴斌

    2013-01-01

    Based on 281 individual plants of F2 population derived from a cross "Yuan Naked Oat"x"555",a genetic linkage map for naked oat (Avena nuda L.) was constructed by 20 AFLP primer pairs,3 SSR primer pairs,and 1 panicle type character.The map was 1544.8 cM in total length with 20.1 cM for the average distance between neighboring markers.92 AFLP markers,3 SSR markers,and 1 morphological trait were mapped on 19 linkage groups,which contained 2-14 markers and varied in size from 23.7 cM to 276.3 cM with an average of 81.3 cM.The segregation ratio of panicle type fitted to 3:1,and 11 AFLP markers demonstrated distorted segregation with the percentage of 11.5%.The results provided a framework of genetic linkage map for naked oat (Avena nuda L.),which was the theoretical basis for QTL mapping,molecular breeding,and comparative genomics in naked oat research.%以元莜麦和555杂交得到的281个F2单株为作图群体,利用20对AFLP引物、3对SSR引物和1个穗型性状构建了一张大粒裸燕麦遗传连锁图.该图谱全长1544.8 cM,包含19个连锁群,其上分布有92个AFLP标记、3个SSR标记和1个穗型形态标记,不同连锁群标记数为2 ~14个,长度在23.7 ~276.3 cM之间,平均长度为81.3 cM,标记间平均距离为20.1 cM.穗型标记分离比符合3:1,11个AFLP标记表现为偏分离,偏分离比为11.5%.该图谱符合遗传连锁框架图的要求,为今后大粒裸燕麦的QTL定位、分子标记辅助育种和比较基因组学等研究奠定基础.

  1. 紫花苜蓿和裸燕麦混贮发酵品质和营养成分分析%Quality of mixed naked oats (Avena nuda)and alfalfa (Medicago sativa)silage

    Institute of Scientific and Technical Information of China (English)

    葛剑; 杨翠军; 杨志敏; 白雪梅; 赵海香; 刘贵河

    2015-01-01

    以新鲜及晾晒后的紫花苜蓿和裸燕麦为原料,分别设计紫花苜蓿单贮、裸燕麦单贮、裸燕麦∶紫花苜蓿=1∶1、裸燕麦∶紫花苜蓿=2∶1及裸燕麦∶紫花苜蓿=1∶2混贮等5个处理,青贮60 d 后,对其感官评分、发酵品质和营养成分等进行分析。结果表明,鲜贮苜蓿在感官评定、V-Score 及 Kaiser 评价体系中表现一般,而晾晒及混合青贮各处理组均能不同程度提高青贮评分。晾晒和混合青贮对发酵品质产生影响,两者显著或极显著降低 pH值、PA(丙酸)、AA(乙酸)、BA(丁酸)和 AN/TN(氨态氮/总氮)含量(P <0.01或 P <0.05),显著或极显著升高LA(乳酸)含量(P <0.01或 P <0.05),其中以裸燕麦∶紫花苜蓿=2∶1降低或升高最明显。晾晒对青贮饲料的干物质、可溶性碳水化合物及粗灰分含量具有显著或极显著影响(P <0.05或 P <0.01),对其他养分含量影响不大(P >0.05)。混合比例对青贮饲料的所有发酵项目均有极显著影响(P <0.01),对粗蛋白质、可溶性碳水化合物及中性洗涤纤维含量具有显著或极显著影响(P <0.05或 P <0.01),对其他养分指标影响不显著(P >0.05)。晾晒和混合青贮的交互作用可显著提高青贮饲料的发酵品质,但对养分含量影响不明显。因此,从青贮后感官评价、发酵品质及养分含量来看,紫花苜蓿和裸燕麦混合青贮品质优于单独青贮,且晾晒相对于鲜贮更易于青贮,而无论晾晒和鲜贮,当裸燕麦∶紫花苜蓿=2∶1时,青贮品质均优于其他各组。%Using sun-dried or fresh alfalfa and naked oats as materials,five silages,including alfalfa silage,na-ked oats silage,three mix silage,(alfalfa∶naked oats =1∶1,alfalfa∶naked oats =1 ∶2 and alfalfa∶naked oats =2 ∶1 ),were prepared.After 60 days ensiling,the sensory

  2. A novel piperazine-bis(rhodamine-B)-based chemosensor for highly sensitive and selective naked-eye detection of Cu{sup 2+} and its application as an INHIBIT logic device

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Zebin; Li, Haizhen; Guo, Dan; Liu, Yan; Tian, Zhang; Yan, Shiqiang, E-mail: yansq@lzu.edu.cn

    2015-11-15

    Abstact: We report the design and synthesis of a new piperazine-bis(rhodamine-B) (RB-P-RB)-based indicator for selective detection of Cu{sup 2+} ion. Optical sensing behavior toward various metal ions including alkali, alkaline earth and transition metal ions (Na{sup +}, K{sup +}, Ba{sup 2+}, Mg{sup 2+}, Ca{sup 2+}, Zn{sup 2+}, Cd{sup 2+}, Mn{sup 2+}, Co{sup 2+}, Ni{sup 2+}, Pb{sup 2+}, Cu{sup 2+}, Hg{sup 2+}, and Ag{sup +}) were investigated by UV–vis and fluorescence spectroscopy in ethassnol solution. The indicator showed highly selective and sensitive colorimetric and “turn-on” fluorescence enhancement responses toward Cu{sup 2+} ion owing to the ring-opening structure of the rhodamine spirolactam. The significant change from colorless to pink upon the addition of Cu{sup 2+} could make it a suitable “naked-eye” indicator for Cu{sup 2+}. Furthermore, a possible ring-opening mechanism (off-on) of the rhodamine spirolactam induced by Cu{sup 2+} binding is supported by Job plot, ESI-mass, FT-IR, and {sup 1}H NMR. More significantly, the probe displayed highly selective Cu{sup 2+}-amplified absorption in the presence of Cu{sup 2+} ions. Finally, using Cu{sup 2+} and EDTA as inputs and the fluorescence emission intensity as output, an INHIBIT logic gate can be constructed at the molecular level. - Highlights: • A novel piperazine-bis(rhodamine-B)-based sensor for selective detection of Cu{sup 2+} ion was synthesized via simple synthetic procedures. • The probe exhibited highly selective and sensitive colorimetric and “turn on” fluorescence enhancement responses to Cu{sup 2+}. • The probe can serve as a reversible and selective “naked eye” indicator for Cu{sup 2+} ions in ethanol solution. • The probe can be utilized to construct an INHIBIT logic gate at the molecular level. • The probe displays highly selective Cu{sup 2+}-amplified absorption in ethanol solution.

  3. Crescimento e desempenho de linhagens de aves pescoço pelado criadas em sistema semi-confinado Growth and performance of naked neck broiler reared in free-range system

    Directory of Open Access Journals (Sweden)

    Leilane Rocha Barros Dourado

    2009-06-01

    Full Text Available Este trabalho foi realizado para avaliar o crescimento e desempenho de duas linhagens de aves pescoço pelado. Foram utilizadas 400 aves, distribuídas em um delineamento inteiramente casualizado, os tratamentos consistiram de um esquema fatorial 2 x 2 (linhagens x sexo, com cinco repetições de 20 aves cada. Foram utilizadas aves Pescoço Pelado das linhagens Sasso e ISA Label. As aves receberam rações formuladas à base de milho e de farelo de soja para atender às exigências nutricionais. Foi determinado o ganho de peso (GP, o consumo de ração (CR e a conversão alimentar (CA aos 28, 56 e 84 dias de idade e o rendimento de carcaça e de partes aos 84 dias de idade. Não foi observada interação entre sexo e linhagem (P>0,05 para nenhuma das variáveis de desempenho analisadas. Para CR e GP houve diferenças significativas (PA trial was carried out to evaluate the growth and performance of naked neck birds. Four hundred birds were distributed into four treatments in factorial arrangement (2 strains x 2 genders, with five replicates of 20 birds each. The naked neck strains evaluated were Sasso and ISA Label. The birds were fed a diet formulated with corn and soybean meal according to the nutritional requirements. Food intake, body weight gain and food conversion were determined at 28, 56, and 84 days as well as the yield of carcass and cuts at 84 days. No interaction was observed between gender and strain (P>0.05. Food intake and body weight gain were different (P <0.05 between the strains and between the genders. Sasso strain presented higher performance than ISA Label. The parameters estimated for Gompertz equation, the weigh at maturity (Wm and time that the growth rate is maximum (t* were higher and maturity rate (b was lower for Sasso strain than those of Isa Label strain. These results indicated that the Isa Label was more precocious than Sasso strain. The females showed better breast yield while the males showed better thigh and

  4. Evolutionary adaptation of the sensitivity of connexin26 hemichannels to CO2.

    Science.gov (United States)

    de Wolf, Elizabeth; Cook, Jonathan; Dale, Nicholas

    2017-02-08

    CO2 readily combines with H2O to form [Formula: see text] and H(+) Because an increase of only 100 nM in the concentration of H(+) (a decrease of 0.1 unit of pH) in blood can prove fatal, the regulated excretion of CO2 during breathing is an essential life-preserving process. In rodents and humans, this vital process is mediated in part via the direct sensing of CO2 via connexin26 (Cx26). CO2 binds to hemichannels of Cx26 causing them to open and allow release of the neurotransmitter ATP. If Cx26 were to be a universal and important CO2 sensor across all homeothermic animals, then a simple hypothesis would posit that it should exhibit evolutionary adaptation in animals with different homeostatic set points for the regulation of partial pressure of arterial CO2 (PaCO2). In humans and rats, PaCO2 is regulated around a set point of 40 mmHg. By contrast, birds are able to maintain cerebral blood flow and breathing at much lower levels of PaCO2 Fossorial mammals, such as the mole rat, live exclusively underground in burrows that are both hypoxic and hypercapnic and can thrive under very hypercapnic conditions. We have therefore compared the CO2 sensitivity of Cx26 from human, chicken, rat and mole rat (Heterocephalus glaber). We find that both the affinity and cooperativity of CO2 binding to Cx26 have been subjected to evolutionary adaption in a manner consistent with the homeostatic requirements of these four species. This is analogous to the evolutionary adaptation of haemoglobin to the needs of O2 transport across the animal kingdom and supports the hypothesis that Cx26 is an important and universal CO2 sensor in homeotherms.

  5. 社会转型时期的爱情——评电视连续剧《裸婚时代》%Love in the Time of Social Transformation --Comment on TV Series Naked Wedding

    Institute of Scientific and Technical Information of China (English)

    贾斌武; 杨梦晨

    2012-01-01

    "The bare marriage" is an increasingly common social phenomenon nowadays,which is a product during the period of social transformation.The Naked Wedding's success just lies in its threedimensional reflection of the collision of multiple values, but it does not lead us towards a world filled with conflicts.In the dialogue of multiple values, the mainstream values were highlighted.In addition, the shaping of space in the drama text plays an important role.%“裸婚”这一愈来愈普遍的社会现象本身即是当下中国社会文化转型时期的产物。《裸婚时代》的成功之处恰在于它立体的呈现了社会转型期多种价值观的碰撞,但它并没有将我们引向永无止境的冲突,而是在多种价值观的交流对话中突出了主流价值观。此外,空间塑造也在电视剧文本中起着重要的作用,通过不同空间关系的营造,其为我们呈现了长久以来被隐匿的阶级话语。

  6. The northern naked-tailed armadillo in the Lacandona rainforest, Mexico: new records and potential threats Armadillo de cola desnuda en la selva lacandona, México: nuevos registros y amenazas potenciales

    Directory of Open Access Journals (Sweden)

    Arturo González-Zamora

    2012-06-01

    Full Text Available We review historic occurrences in Mexico of one of the least known Xenarthra of Mesoamerica - the northern naked-tailed armadillo (Cabassous centralis Miller, 1899. We document 6 new records in the Lacandona rainforest, southern Mexico, and through interviews with local people, we assess potential threats for this species in the region. In addition to being locally rare, our results show that hunting and death by road-kill may be major threats to this species in the region.Se revisaron los registros históricos en México de uno de los xenartros menos conocidos de Mesoamérica - el armadillo de cola desnuda (Cabassous centralis Miller, 1899. Se documentan 6 nuevos registros en la selva lacandona, sur de México. A través de entrevistas con pobladores locales, se evaluaron las amenazas potenciales para esta especie en la región. Además de ser localmente rara, nuestros resultados muestran que la cacería y las muertes por atropellamiento pueden ser las principales amenazas para esta especie en la región.

  7. Preparation of a Wine Yeast-fermented Beverage from Naked Oat%葡萄酒酵母菌发酵莜麦饮料生产工艺

    Institute of Scientific and Technical Information of China (English)

    王健; 罗永华; 刘媛; 兰凤英

    2012-01-01

    以糖化莜麦汁为原料,探讨了葡萄酒酵母菌发酵莜麦饮料的生产工艺。在单因素试验的基础上,采用正交试验得到葡萄酒酵母菌发酵莜麦饮料的优化工艺条件:接种量4%,28℃下发酵20 h。木糖醇添加量为7%;果胶添加量为0.45%。%After saccharificatic was used as the main raw material,the production process of the fermented naked oat juice was studied.On the basis of single factor experiment,the optimal process conditions of the fermentation beverage were determined by orthogonal experiment as follows: 4% wine yeast,28℃of wine yeast fermentation for 20 h,7% xylitol,0.45% pectin.

  8. A selective naked-eye chemosensor derived from 2-methoxybenzylamine and 2,3-dihydroxybenzaldehyde - synthesis, spectral characterization and electrochemistry of its bis-bidentates Schiff bases metal complexes

    Science.gov (United States)

    Djouhra, Aggoun; Ali, Ourari; Ramiro, Ruiz-Rosas; Emilia, Morallon

    2017-09-01

    A new colorimetric receptor HL, acting as a bidentate Schiff base ligand, has been synthesized by condensation of 2-methoxybenzylamine on 2,3-dihydroxybenzaldehyde in a methanolic solution. Interestingly, this chelating agent can selectively detect Cu2 +, Co2 +, Fe2 + and Fe3 + ions with a simple and an easy-to-make, well defined naked-eye visible color changes in two different solvents like acetonitrile and methanol. This bidentate ligand coordinates three metal ions of Co(II), Cu(II) and Fe(II) via nitrogen and oxygen atoms. The molecular structures of the synthesized compounds were elucidated by various physicochemical properties such as the elemental analysis, FT-IR, HNMR, UV-Vis and the Mass spectrometry. The resulting general formulae [M(L)2·H2O] (M(II) = Cu, Fe, Co) are proposed as mononuclear complexes. The solvatochromism properties of these compounds were studied with their absorption spectra using different solvents as methanol (MeOH), acetonitrile (AN), tetrahydrofuran (THF), dimethylformamid (DMF), dimethylsulfoxid (DMSO) and dichloromethane (DC). The Electrochemical behavior of copper complex was explored in DMF solutions by cyclic voltammetry (CV) with two working electrodes: glassy carbon (GC) and platinum electrode (Pt). This study reveals that copper complex shows successively two redox systems as CuIII/II and CuII/I. The FeIII/II and CoII/I redox systems have also been studied in DMF and DMSO media.

  9. Stability analysis of a model gene network links aging, stress resistance, and negligible senescence.

    Science.gov (United States)

    Kogan, Valeria; Molodtsov, Ivan; Menshikov, Leonid I; Shmookler Reis, Robert J; Fedichev, Peter

    2015-08-28

    Several animal species are considered to exhibit what is called negligible senescence, i.e. they do not show signs of functional decline or any increase of mortality with age. Recent studies in naked mole rat and long-lived sea urchins showed that these species do not alter their gene-expression profiles with age as much as other organisms do. This is consistent with exceptional endurance of naked mole rat tissues to various genotoxic stresses. We conjectured, therefore, that the lifelong transcriptional stability of an organism may be a key determinant of longevity. We analyzed the stability of a simple genetic-network model and found that under most common circumstances, such a gene network is inherently unstable. Over a time it undergoes an exponential accumulation of gene-regulation deviations leading to death. However, should the repair systems be sufficiently effective, the gene network can stabilize so that gene damage remains constrained along with mortality of the organism. We investigate the relationship between stress-resistance and aging and suggest that the unstable regime may provide a mathematical basis for the Gompertz "law" of aging in many species. At the same time, this model accounts for the apparently age-independent mortality observed in some exceptionally long-lived animals.

  10. Region-specific associations between sex, social status, and oxytocin receptor density in the brains of eusocial rodents.

    Science.gov (United States)

    Mooney, S J; Coen, C W; Holmes, M M; Beery, A K

    2015-09-10

    Naturally occurring variations in neuropeptide receptor distributions in the brain contribute to numerous mammalian social behaviors. In naked mole-rats, which live in large social groups and exhibit remarkable reproductive skew, colony-related social behaviors vary with reproductive status. Here we examined whether variation in social status is associated with variations in the location and/or density of oxytocin binding in this species. Autoradiography was performed to assess forebrain oxytocin receptor (OTR) densities in breeding and non-breeding naked mole-rats of both sexes. Overall, males exhibited higher OTR binding in the medial amygdala in comparison to females. While there were no main effects of reproductive status in any region, a sex difference in OTR binding in the nucleus accumbens was mediated by status. Specifically, breeding males tended to have more OTR binding than breeding females in the nucleus accumbens, while no sex difference was observed in subordinates. These effects suggest that oxytocin may act in a sex- and region-specific way that corresponds to reproductive status and associated social behaviors.

  11. Starch Quality Analysis of Different Naked Oat Cultivars from Different Regions%不同地区不同裸燕麦品种淀粉品质分析

    Institute of Scientific and Technical Information of China (English)

    郑建梅; 曹莹莉; 胡新中; 徐明

    2012-01-01

    In order to analyze the effects of cultivation conditions on starch quality of naked oat , ten naked oat cultivars which were planted in Inner Mongolia and Hebei, the two main oat planting regions in China, were used as the materials. Resistant starch (RS), total starch (TS), starch pasting properties and starch digestion simulation in vitro of oat were determined. With multiple comparison methods, differences among the ten samples and between the two areas were analyzed. The results indicated that there are significant differences for the resistant starch, total starch, peak viscosity, breakdown and setback among the oat samples of each region. There are significant differences for the resistant starch and total starch between two regions, there are more significant differences in starch digestion simulation in vitro and most starch pasting properties between two regions. The starch di- gestion simulation in vitro and pasting temperature of Inner Mongolia samples are significant higher than those of Hebei. The peak viscosity, breakdown and setback of Inner Mongolia samples are significant lower than those of Hebei.%为研究不同生长环境对燕麦淀粉品质的影响,分别选取同时在我国两大燕麦主产区内蒙古和河北省种植的10个裸燕麦品种,测定其抗性淀粉含量、总淀粉含量、糊化特性和淀粉水解率,通过多重比较分析品种的变异性和地区之间的差异性。结果表明,两地区内参试样品间的抗性淀粉、总淀粉、峰值黏度、破损值和回生值的差异均较大;两地区之间,抗性淀粉和总淀粉含量存在显著差异(P〈0.05),淀粉水解率以及大部分糊化特性存在极显著差异(P〈0.01);内蒙古地区种植的燕麦样品淀粉水解率、糊化温度极显著(P〈0.01)高于河北地区种植的燕麦样品,而峰值黏度、破损值、回生值极显著(P〈0.01)低于河北地区的样品。

  12. Analysis on growth characters and production levels of two species of naked oat (Avena nuda) in Henan Province%2种莜麦在河南的生长特点与生产水平的分析

    Institute of Scientific and Technical Information of China (English)

    李明

    2009-01-01

    To found a possibility and necessity of introduce naked oat (Avena nuda) into Hennan Province, growth characters and production levels of 2 species, Neinongda Oats-l and Neinongda Oats-2, from Inner Mongolia were studied at yellow river beach in Henan Province. Grain yields were 457.6 g/m~2 and 481.7 g/m~2, and forage yields (DM) were 1 608.1 g/m~2 and 1 472.8 g/m~2. These were higher than that in Inner Mongolia. This imply that the two species are adapted to the soil and the climate of the area.%把来自内蒙古的2个莜麦Avena nuda优良品种内农大莜1号和内农大莜2号,引种到河南省黄河滩区来种植,研究其生长特点以及生产水平,目的是通过与原产地生产水平的比较,来探索这2个莜麦品种在河南推广的可能性和必要性.结果表明:莜麦能很好地适应河南省黄河滩区土壤和气候条件,表现出生长速度快和生产水平高的特点,内农大莜1号和内农大莜2号的饲草干物质产量分别为1 608.1和1 472.8 g/m~2,果实产量分别为457.6和481.7 g/m~2,均显著地高于原产地的生产水平.

  13. 裸眼3D LCD动静态图像显示的设计研究%Design and research on displaying images and texts on naked-eye 3 D LCD

    Institute of Scientific and Technical Information of China (English)

    宋凡; 梁发云; 刘敏; 许鹏; 王兴鹏

    2015-01-01

    The naked-eye 3 D LCD could be applied to various image display device and can also be used to display the 2D and 3D graphics . ×ith the continuous improvement of the TFT LCD resolution,it needed better hardware technology to display the full image correctly. According to the actual demand of displaying images and texts ,the LCD driving interfaces were designed in this paper based on FPGA. And the circuital timing sequence was generated by the hardware logic algorithm to send pixel data correctly through the RGB line. The results showed that the design could achieve stably performance and acquire a dynamic display of images and texts by online de-bugging .%裸眼3D LCD能够应用到多种图像显示器件中,满足二维及三维图形显示。在TFT LCD的分辨率不断提高的情况下,也对正确显示完整图像的硬件技术提出了更高的要求。根据图像与文字显示的现实需求,使用FP-GA来驱动液晶屏接口。并用硬件逻辑算法产生LCD驱动的时序电路,配合RGB数据线完成像素的正确传送。在线调试结果表明该设计能达到稳定的显示效果,实现了图像和字符的动态显示。

  14. Naked Stony Corals: Skeleton Loss in Scleractinia

    Energy Technology Data Exchange (ETDEWEB)

    Medina, Monica; Collins, Allen G.; Takaoka, Tori L.; Kuehl,Jennifer; Boore, Jeffrey L.

    2005-12-01

    Hexacorallia includes the Scleractinia, or stony corals, characterized by having an external calcareous skeleton made of aragonite, and the Corallimorpharia, or mushroom corals, that lack such a skeleton. Although each group has traditionally been considered monophyletic, some molecular phylogenetic analyses have challenged this, suggesting that skeletal features are evolutionarily plastic, and reviving notions that the scleractinian skeleton may be ephemeral and that the group itself may be polyphyletic. Nevertheless, the most comprehensive phylogenetic study of Hexacorallia supported scleractinian monophyly (REF), and so this remains controversial. In order to resolve this contentious issue, we sequenced the complete mitochondrial genome sequences of nine scleractinians and four corallimorpharians and performed phylogenetic analysis that also included three outgroups (an octocoral and two sea anemones). Our data provide the first strong evidence that Scleractinia is paraphyletic and that the Corallimorpharia is derived from within the group, from which we conclude that skeletal loss has occurred in the latter group secondarily. It is possible that a driving force in such skeletal loss could be the high levels of CO{sub 2} in the ocean during the mid-Cretaceous, which would have impacted aragonite solubility. We estimate from molecular divergence measures that the Corallimorpharia arose in the mid-Cretaceous, approximately 87 million years ago (Ma), supporting this view. These data also permit us to date the origin of Scleractinia to 265 Ma, narrowing the gap between the group's phylogenetic origin and its earliest fossil record.

  15. Go Naked: Diapers Affect Infant Walking

    OpenAIRE

    Cole, Whitney G.; Lingeman, Jesse M.; Adolph, Karen E.

    2012-01-01

    In light of cross-cultural and experimental research highlighting effects of childrearing practices on infant motor skill, we asked whether wearing diapers, a seemingly innocuous childrearing practice, affects infant walking. Diapers introduce bulk between the legs, potentially exacerbating infants’ poor balance and wide stance. We show that walking is adversely affected by old-fashioned cloth diapers, and that even modern disposable diapers—habitually worn by most infants in the sample—incur...

  16. Go Naked: Diapers Affect Infant Walking

    Science.gov (United States)

    Cole, Whitney G.; Lingeman, Jesse M.; Adolph, Karen E.

    2012-01-01

    In light of cross-cultural and experimental research highlighting effects of childrearing practices on infant motor skill, we asked whether wearing diapers, a seemingly innocuous childrearing practice, affects infant walking. Diapers introduce bulk between the legs, potentially exacerbating infants' poor balance and wide stance. We show that…

  17. Effect of heat exposure on the thermoregulatory responses of selected naked neck chickens Efeito da exposição ao calor na resposta termorregulatória de aves de pescoço pelado selecionadas

    Directory of Open Access Journals (Sweden)

    C.M. Mazzi

    2002-02-01

    Full Text Available The variation in cloacal temperature, body weight loss and expression of the 70 kDa heat shock protein (Hsp70 in three naked neck broiler genotypes during heat stress were studied. Twelve birds of each genotype (Na/Na, Na/na and na/na were reared to market weight (approximately 2.1kg at thermoneutral temperature. Six birds from each group served as controls and the remaining six underwent gradual heat stress (from 28ºC to 36ºC. Cloacal temperature and body weight were measured before and after exposure to heat. Liver samples were collected and Hsp70 levels were quantified using western blotting with monoclonal anti-chicken Hsp70 antibody. Heterozygous (Na/na birds had a significantly lower cloacal temperature variation and less body weight loss during heat stress than the other genotypes. There was no significant difference in the Hsp70 levels among the genotypes. Heterozygous birds (Na/na appeared to have a slight advantage over the other genotypes during gradual heat stress, perhaps because of a heterotic effect.Estudaram-se o efeito do estresse térmico sobre a temperatura cloacal, a perda de peso corporal e a expressão da proteína de estresse de 70 kDa (Hsp70 em três genótipos de galinhas de pescoço-pelado. Foram usadas 12 aves de cada genótipo (Na/Na, Na/na e na/na, com peso corporal médio de 2,100kg e criadas em temperatura termoneutra. Seis aves de cada grupo serviram como controle e as seis restantes foram submetidas a estresse térmico gradativo (28ºC - 36ºC. A temperatura cloacal e o peso das aves foram avaliados antes e depois do estresse. Amostras de fígado foram coletadas e os níveis de Hsp70 foram quantificados por "western blotting" com anticorpo monoclonal específico para Hsp70 de galinha. As aves heterozigotas (Na/na tiveram variação de temperatura cloacal significativamente menor e menor perda de peso corporal durante o estresse térmico do que as dos outros genótipos. Não foi observada diferen

  18. 生态马克思主义视角下的《裸者与死者》%An Ecological Marxist Interpretation of The Naked and the Dead

    Institute of Scientific and Technical Information of China (English)

    徐夫玲

    2014-01-01

    在《裸者与死者》中,梅勒描绘了一幅生态严重失衡的图景,遭受一次次战争洗礼满目疮痍的自然环境,人和自然之间疏离以及在非常态的环境下,人对金钱权力等的追逐所暴露出的人类世界种种深层不道德现象,这一切都深刻揭示当今社会人与自然之间的异化,印证了生态马克思主义关于资本主义反生态的种种趋势。从生态马克思主义视角解读这部小说,不仅可以更深刻地理解作家的创作意图和作品的内涵,而且可以给读者提供一个全新的视角去审视造成人类的悲惨境遇的成因。%In The Naked and the Dead,Mailer displays the scenes of modern society where ecology is severely damaged with the devastated natural environment because of war time and time again,estrangement between human beings and nature,and man's im-morality and acquisitive desires for money and power in the abnormal state of life.All these indicate the alienation between human be-ings and nature in the nowadays society,which echoes the eco-Marxist argument about counter-ecological tendencies.If we inter-pret the novel within the framework of eco-Marxism,we can not only obtain a thorough understanding of the implication of the no-vel,but also examine the novelist's creation intention,thus revealing his foresightful ecological consciousness and his reflection on the way out of the ecological crisis in the modern society in a brand new perspective.

  19. Fluorescence Property of a Chemical Probe for Naked-Eye and Detection of Fe3+%牛奶水溶液的偏振三维荧光光谱研究

    Institute of Scientific and Technical Information of China (English)

    兰秀风; 顾春峰; 于银山; 卢礼萍

    2012-01-01

    测得由伊利、蒙牛公司生产的纯牛奶、高钙奶、高钙低脂奶的偏振三维荧光光谱,发现在激发偏振片和发射偏振片取向平行时,偏振荧光强度高于取向垂直时的值.通过一个简单的模型估算了各种牛奶样品等效荧光团的激发偶极子和发射偶极子之间的夹角,均小于40°,且高钙低脂奶对应荧光团激发偶极子和发射偶极子之间的夹角略小于纯牛奶和高钙奶.实验还发现,在激发偏振片和发射偏振片取向平行时,高钙低脂奶的偏振荧光强度明显高于纯牛奶和高钙奶,与理论计算相符.%A higher selective and sensitive probe for the detection of Fe( m ) in aqueous media was made using 2,4-Diisocyanato-toluene (TDD as a bridge to couple Fesd nanoparticles(NPs) and Rhodamine-6G hydrazide. The characterization of composite materials with Infrared spectra(IR), Thermal Gravimetric analysis(TGA) and Transmission Emission Microscopy(TEM) pointsto the graft of Rhodamine-6G hydrazide onto the surface of the Fe3 O4. The obvious color change of the probe solution from light grey to pink upon the addition of Fe3+ demonstrated the probe could be used for "naked-eye" detection of Fe3+ in water at pH 7. The presence of 1 equivalent (l0μmol· L-1 μm) of each of these metal ions, including Mn2+ , Ni2+ , Y" , Eu3+ , Ce3+ , La3+ , Pr3"1", Cd2+ , Cr34", Sm3+ , Fe2+ , Cu2+and Zn2+ ions, did not demonstrate any obvious fluorescence change of the probe water solution, which confirmed the probe was a probe with remarkable selectivity for Fe3+. And the fluorescence images of HeLa cells in physiological solutions after incubation with Fe3+ and then further incubated with the probe leading to a strong intracellu-lar fluorescence, which suggested the probe could penetrate the HeLa cell membrane and could respond to Fe3+ in intracellular within living cells.

  20. Long-lived cancer-resistant rodents as new model species for cancer research

    Directory of Open Access Journals (Sweden)

    Jorge eAzpurua

    2013-01-01

    Full Text Available Most rodents are small and short-lived, but several lineages have independently evolved long lifespans without a concomitant increase in body mass. Most notably, the two subterranean species naked mole rat (NMR and blind mole rat (BMR which have maximum lifespans of 32 and 21 years respectively. The longevity of these species has sparked interest in the tumor suppression strategies that may have also evolved, because for many rodent species (including mice, rats, guinea pigs, gerbils and hamsters tumors are major source of late-life mortality. Here, we review the recent literature on anticancer mechanisms in long-lived rodents. Both NMR and BMR seem to have developed tumor defenses that rely on extra-cellular signals. However, while the NMR relies on a form of contact inhibition to suppress growth, the BMR evolved a mechanism mediated by the release of interferon and rapid necrotic cell death. Although both organisms ultimately rely on canonical downstream tumor suppressors (pRB and p53 the studies reveal species can evolve different strategies to achieve tumor-resistance. Importantly, studies of these cancer-resistant rodents may benefit human health if such mechanisms can be activated in human cells.

  1. Sensitivity of primary fibroblasts in culture to atmospheric oxygen does not correlate with species lifespan

    Science.gov (United States)

    Patrick, Alison; Seluanov, Michael; Hwang, Chaewon; Tam, Jonathan; Khan, Tanya; Morgenstern, Ari; Wiener, Lauren; Vazquez, Juan M.; Zafar, Hiba; Wen, Robert; Muratkalyeva, Malika; Doerig, Katherine; Zagorulya, Maria; Cole, Lauren; Catalano, Sophia; Lobo Ladd, Aliny AB; Coppi, A. Augusto; Coşkun, Yüksel; Tian, Xiao; Ablaeva, Julia; Nevo, Eviatar; Gladyshev, Vadim N.; Zhang, Zhengdong D.; Vijg, Jan; Seluanov, Andrei; Gorbunova, Vera

    2016-01-01

    Differences in the way human and mouse fibroblasts experience senescence in culture had long puzzled researchers. While senescence of human cells is mediated by telomere shortening, Parrinello et al. demonstrated that senescence of mouse cells is caused by extreme oxygen sensitivity. It was hypothesized that the striking difference in oxygen sensitivity between mouse and human cells explains their different rates of aging. To test if this hypothesis is broadly applicable, we cultured cells from 16 rodent species with diverse lifespans in 3% and 21% oxygen and compared their growth rates. Unexpectedly, fibroblasts derived from laboratory mouse strains were the only cells demonstrating extreme sensitivity to oxygen. Cells from hamster, muskrat, woodchuck, capybara, blind mole rat, paca, squirrel, beaver, naked mole rat and wild-caught mice were mildly sensitive to oxygen, while cells from rat, gerbil, deer mouse, chipmunk, guinea pig and chinchilla showed no difference in the growth rate between 3% and 21% oxygen. We conclude that, although the growth of primary fibroblasts is generally improved by maintaining cells in 3% oxygen, the extreme oxygen sensitivity is a peculiarity of laboratory mouse strains, possibly related to their very long telomeres, and fibroblast oxygen sensitivity does not directly correlate with species' lifespan. PMID:27163160

  2. 播种量和施肥对丘陵红壤区裸燕麦产量和主要性状的影响%Effects of Seeding Rate and Fertilization on Yield and Main Traits of Naked Oat in Hilly Red Soil Area

    Institute of Scientific and Technical Information of China (English)

    张昆; 刘小三; 郑伟; 雷礼文

    2012-01-01

    在江西丘陵红壤区研究了不同播种量和施肥对裸燕麦品种白燕8号和坝莜6号产量及主要性状的影响.结果表明:这两个品种的产量差异不显著;不同播种量和施肥对燕麦产量均有极显著的影响;白燕8号和坝莜6号在播种量75 kg/hm2、基肥施复合肥525 kg/hm2、追肥施尿素和氯化钾各60 kg/hm2的条件下可以获得最高的产量.%A field experiment with multi -factor (variety, seeding rate, and fertilization) design was conducted to study the influences of seeding rate and fertilization on the yield and main traits of naked oat in hilly red soil area of Jiangxi. The naked oat varieties Baiyan No. 8 and Bayou No. 6 were taken as the test materials. The results indicated that there was no significant difference in the yield between these two varieties, while both seeding rate and fertilization very significantly affected the oat yield. The conditions for the highest yield of Baiyan No. 8 and Bayou No. 6 was obtained as follows: seeding rate 75 kg/hm2, compound fertilizer 525 kg/ hm as base fertilizer, and 60 kg/hm2 urea and 60 kg/hm2 potassium chloride as top dressing.

  3. New data on programmed aging - slow phenoptosis.

    Science.gov (United States)

    Skulachev, M V; Skulachev, V P

    2014-10-01

    This review summarizes the latest data on biochemistry and physiology of living organisms. These data suggest that aging, i.e. coordinated age-dependent weakening of many vital functions leading to gradual increase in the probability of dying, is not common to all organisms. Some species have been described whose probability of death does not depend on age or even decreases with age, this being accompanied by constant or increasing fertility. In the case of the naked mole rat (a non-aging mammal), a mechanism has been identified that protects this animal from cancer and the most common age-related diseases. The high molecular weight polysaccharide hyaluronan, a linear polymer composed of multiple repeated disaccharide of glucuronic acid and glucosamine, plays the key role in this mechanism. Hyaluronan is accumulated in the intercellular spaces in the organs and tissues of the naked mole rat. This polysaccharide provides early contact inhibition of cell division (anti-cancer effect). In addition, hyaluronan prevents the development of certain types of apoptosis, in particular, those induced by reactive oxygen species (ROS) (geroprotective effect preventing ROS-induced decrease in cellularity in the organs and tissues of aging organisms). Extraordinary longevity of the naked mole rat (over 30 years, which is long for a rodent the size of a mouse) is connected to its eusocial lifestyle, when only the "queen" and its few "husbands" breed, while the huge army of non-breeding "subordinates" provide the "royal family" with protection from predators, food, and construction and maintenance of an underground labyrinth size of a football field. This way of life removes the pressure of natural selection from the "family" and makes aging - the program that is counterproductive for the individual but increases "evolvability" of its offspring - unnecessary. The example of the naked mole rat demonstrates the optional character of the aging program for the organism. Many facts

  4. Rodents for comparative aging studies: from mice to beavers.

    Science.gov (United States)

    Gorbunova, Vera; Bozzella, Michael J; Seluanov, Andrei

    2008-09-01

    After humans, mice are the best-studied mammalian species in terms of their biology and genetics. Gerontological research has used mice and rats extensively to generate short- and long-lived mutants, study caloric restriction and more. Mice and rats are valuable model organisms thanks to their small size, short lifespans and fast reproduction. However, when the goal is to further extend the already long human lifespan, studying fast aging species may not provide all the answers. Remarkably, in addition to the fast-aging species, the order Rodentia contains multiple long-lived species with lifespans exceeding 20 years (naked mole-rat, beavers, porcupines, and some squirrels). This diversity opens great opportunities for comparative aging studies. Here we discuss the evolution of lifespan in rodents, review the biology of slow-aging rodents, and show an example of how the use of a comparative approach revealed that telomerase activity coevolved with body mass in rodents.

  5. DETERMINATION INNER MONGOLIA DIFFERENT VARISTIES OF NAKED OATS ONO-STARCH POLYSACCHARIDES CONTENT BY GAS CHROMATOGRAPHY%硅烷化衍生化-气相色谱法测定不同品种莜麦的非淀粉多糖

    Institute of Scientific and Technical Information of China (English)

    许辉; 白国涛; 潘国卿; 赵宇龙

    2013-01-01

    Silylation derivatization-Gas Chromatographic Determination Method for determination of non-starch polysaccharides in naked oats.BaiYan No.2 and no.8 NeiYan no.5 Yan no.08-9 and market no.1 of five kinds of different Inner Mongolia production of naked oats,soluble and insoluble non-starch polysaccharide content were measured.Fat samples with hexane to 80% ethanol to free sugars,and then,in pH =5,HAC buffer using α-amylase and α-glucosidase enzymatic hydrolysis of starch can be divided into soluble non-starch polysaccharides and insoluble non-starch polysaccharides by acid hydrolysis,and the constituent monosacchatides of its non-starch polysaccharides using the silylation derivatization,gas chromatography were determined.The linear correlation coefficient between 0.99584-0.99909,the xylose content in the 1.984-4.384 mg / g,the mannose content between 1.866-3.221 mg / g,the galactose content of 0.454-0.903mg / g,between the glucose content in the 5.31-10.528 mg / g,total 9.614-17.016mg / g.This method is suitable for the determination of naked oats in the soluble and insoluble non-starch polysaccharides.%本文采用硅烷化衍生化-气相色谱法,测定了白燕2号等内蒙古不同品种莜麦中可溶性和不可溶性非淀粉多糖的含量.样品经正己烷去脂肪,80%乙醇去游离糖,在pH =5的HAC缓冲液中用α-淀粉酶和α-葡萄糖苷酶酶解,可得到可溶性非淀粉多糖(SNSP)和不可溶性非淀粉多糖(ISNP).经酸水解后,对其非淀粉多糖的各组成单糖采用硅烷化进行衍生物制备,直接气相色谱法测定.本方法的线性相关系数在0.99584-0.99909之间,木糖含量在1.984-4.384 mg/g之间,甘露糖含量在1.866-3.221 mg/g之间,半乳糖含量在0.454-0.903mg/g之间,葡萄糖含量在5.31-10.528mg/g之间,总量在9.614-17.016mg/g之间.结果表明,该方法适用于莜麦中可溶性和不可溶性非淀粉多糖的测定,为莜麦的深入品质评价及进行产品有效成分提取和利用,

  6. 耕作方式对冀西北栗钙土土壤物理性状及莜麦生长的影响%Effects of tillage mode on chestnut soil’s physical characters and naked oats growth in Northwest Hebei province

    Institute of Scientific and Technical Information of China (English)

    王岩; 刘玉华; 张立峰; 窦铁岭

    2014-01-01

    为了探索不同耕作方式对冀西北栗钙土农田土壤物理性状及莜麦生长的影响,以河北省张北县10 a栗钙土长期定位试验莜麦田为研究对象,研究了免耕、松耕和翻耕对莜麦田土壤容重、土壤含水率、土壤硬度及莜麦生长的影响。结果表明:松耕和翻耕可以显著降低莜麦播种期到拔节期土壤容重,播种期免耕土壤容重1.49 g/cm3,松耕和翻耕分别为1.31和1.30 g/cm3;不同耕作方式对土壤含水率影响不大;免耕显著提高土壤硬度,拔节期免耕土壤硬度58.51kg/cm2,为松耕1.74倍(P<0.05),为翻耕2.53倍(P<0.01);栗钙土土壤硬度与土壤容重、土壤含水率关系模型表明高土壤容重条件下土壤硬度对土壤含水率更敏感,低土壤含水率条件下土壤硬度对土壤容重更敏感;免耕莜麦株高和叶面积生长受到抑制,穗数和穗粒数显著降低,经济产量413.79 kg/hm2,分别为松耕和翻耕的62.27%和51.64%。栗钙土莜麦田免耕与松耕、翻耕相比土壤容重大,土壤硬度高,莜麦产量显著降低;3种耕作方式中,松耕是兼顾生态与经济效益的耕作措施。%Taking a ten-year naked oats field experiment site in Zhangbei county of Hebei province as the test object, this paper studied the effects of different tillage modes (no tillage, subsoiling tillage, and conventional tillage) on the soil physical characteristics and naked oats growth in chestnut soil. The no tillage treatment soil was undisturbed from last year’s harvesting to sowing, with 15-22 cm stubbles; broad-spectrum herbicide was used in soil treatment before planting and sprayed in seedling. The subsoiling tillage treatment plowed the soil after the previous year’s harvest, used the self-developed‘Parallel rod without wall subsoiling plow’ (Patent No.:2011202468728) with a plowed depth of 15-22 cm, and was sowed and fertilized in the same manner as the no tillage

  7. Subterranean sympatry: An investigation into diet using stable isotope analysis

    CSIR Research Space (South Africa)

    Robb, GN

    2012-11-01

    Full Text Available mole-rat (Georychus capensis) and the Cape dune mole-rat (Bathyergus suillus) were examined using stable isotope analysis. Blood, fur and claw samples were collected from 70 mole-rats, in addition to several potential food items, to assess food...

  8. Synthesis and Spectroscopic Study of a Chemosensor for Naked Eye Recognition of Cu2+ and Hg2+%一种裸眼识别Cu2+和Hg2+传感器的合成与性质研究

    Institute of Scientific and Technical Information of China (English)

    曹丽; 钱亚骜; 黄燕; 曹娟; 贾春满; 刘春玲; 张岐; 吕正荣

    2015-01-01

    Compound L ,as the procedural sensor for the detection of Cu2+ and Hg2+ ,was designed and synthesized based on the coumarin‐modified rhodamine derivative .The structure of compound L was characterized by NMR ,high resolution mass spec‐trometry and infrared method .Its sensing behavior toward various metal ions was investigated with absorbance methods .The study found that L had good selectivity and sensitivity for Cu2+ .When addition of various metal ions (Zn2+ ,Hg2+ ,Cu2+ , Fe3+ ,Cd2+ ,Co2+ ,Ni2+ ,Mg2+ ,Ca2+ ,Al3+ ,La3+ ,K+ ,Na+ ,Mn2+ ,Pb2+ and Ag+ ) ,only Cu2+ could induce a visible change of solution from colourless to pink and a new absorption band centered at 534 nm appear ,which indicated that compound L could be used for the naked eye detection of Cu2+ .From UV titration ,the detection limit was about 1.9 × 10-8 mol・ L -1 . Test strips based on L were fabricated ,and this test strips could act as a convenient and efficient Cu2+ test kit .The binding ratio of the complex of L‐Cu2+ was 1∶1 according to the Job’s plot and high resolution mass spectrometer (HRMS) experiments . Moreover ,Upon addition of 1 equiv .EDTA to the mixture of L and Cu2+ in DMSO solution ,colour changed from pink to al‐most colourless ,indicating that the EDTA replaced the receptor L to coordinate with Cu 2+ .Therefore ,L could be classified as a reversible sensor for Cu2+ .In addition ,when adding Hg2+ to L‐Cu2+ complexes ,a visible change of solution from pink to col‐ourless was observed ,while other metal ions didn’t cause this change .Thus ,L‐Cu2+ complex also could be used for the naked eye recognition of Hg2+ ,and the detection limit was calculated about 2.9 × 10-7 mol・L -1 according to the UV titration .Conse‐quently ,this procedural sensor L could be use for the orderly naked eye recognition of Cu2+ and Hg2+ .%设计合成了一种香豆素修饰的罗丹明类衍生物L ,用作程序型Cu2+与Hg2+检测传感器。采用核磁共振谱、高分

  9. The naked spinor a rewrite of Clifford algebra

    CERN Document Server

    Morris, Dennis

    2015-01-01

    This book is about spinors. The whole mathematical theory of spinors is within Clifford algebra, and so this book is about Clifford algebra. Spinor theory is really the theory of empty space, and so this book is about empty space. The whole of Clifford algebra is rewritten in a much simpler form, and so the whole of spinor theory is rewritten in a much simpler form. Not only does this book make Clifford algebra simple and obvious, but it lifts the fog and mirrors from this area of mathematics to make it clear and obvious. In doing so, the true nature of spinors is revealed to the reader, and, with that, the true nature of empty space. To understand this book you will need an elementary knowledge of linear algebra (matrices) an elementary knowledge of finite groups and an elementary knowledge of the complex numbers. From no more than that, you will gain a very deep understanding of Clifford algebra, spinors, and empty space. The book is well written with all the mathematical steps laid before the reader in a w...

  10. Demonstrating the Elliptical Orbit of Mars using Naked Eye Data

    CERN Document Server

    Krisciunas, Kevin

    2016-01-01

    Over the course of eleven months we determined the position of Mars on 45 occasions using a handheld cross staff and two to five bright reference stars of known right ascension and declination on each occasion. On average the observed positions are within 12 arc minutes of the true positions. Given that we took data prior to the start of retrograde motion and well past the end of retrograde motion, we can easily derive the date of opposition to the Sun. We were able to derive the date of perihelion, the orbital eccentricity, and the semi-major axis size of Mars' orbit. We obtain a value of the eccentricity of 0.086 +/- 0.010, which is to be compared to the modern value of 0.0934. Values as low as 0.053 or as high as 0.123 can be rejected at a high confidence level. A simple dataset can be obtained with cardboard and a ruler that demonstrates the elliptical shape of Mars' orbit.

  11. Naked Nature%赤裸的自然

    Institute of Scientific and Technical Information of China (English)

    Samuele Martelli

    2006-01-01

    1986年,一对来自意大利南部的年轻的兄弟,Ennio与Carlo Capasa,在米兰工作很长时间之后,创立一个新的时装店——Costumet National.在短短几年之后,这个服装品牌迅速成为五光十色的意大利时尚界最为著名的品牌之一。Costume National与其他成熟品牌一样崇尚自然存在主义精神,这种精神也深深扎根在两位创立者的血液中。但又有所不同,Costume National受到了非意大利传统的影响。因此,它的形象很难与常规的民族风尚联系在一起。

  12. productive and physiological adaptive responses of ethiopian naked ...

    African Journals Online (AJOL)

    Admin

    in egg production (33%), feed intake (15%) and shell thickness (24.3%). The effect of heat ..... Exchange Service (KAAD) of Germany for covering his scholarship expenses. .... at high ambient temperature in genetically fat and lean broiler ...

  13. Waiting for the Barbarians and the Naked Emperor's Chicken.

    Science.gov (United States)

    Taylor, Paul

    2003-01-01

    Explores the reasons behind the ubiquity of managerial ethics among academics whose own teaching and research often critiques those same values. Discusses the ways in which academics either promote or supinely acquiesce to managerialist processes and the consequences of this for educational values. (EV)

  14. The naked toy model of a jumping ring

    Science.gov (United States)

    Donoso, Guillermo; Ladera, Celso L.

    2014-01-01

    We present a comprehensive analytical model of the well-known jumping ring—in fact an improved version of that system--as well as the experimental results that validate the model. Particular attention is paid to the magnetic driving force, whose explicit dependences upon the phase, amplitude and frequency of the exciting current we manage to separate experimentally and plot, so that it becomes evident how the magnetic force on the ring actually arises and evolves in time. We are able to measure not only the large Foucault currents that arise in the ring, but also the magnetic field generated by the ring itself in spite of the presence of the comparable magnetic field in which the ring moves.

  15. Waiting for the Barbarians and the Naked Emperor's Chicken.

    Science.gov (United States)

    Taylor, Paul

    2003-01-01

    Explores the reasons behind the ubiquity of managerial ethics among academics whose own teaching and research often critiques those same values. Discusses the ways in which academics either promote or supinely acquiesce to managerialist processes and the consequences of this for educational values. (EV)

  16. Construction of SSR Genetic Linkage Map and Analysis of QTLs Related toβ-glucan Content of Naked Oat (Avena nuda L.)%裸燕麦SSR标记连锁群图谱的构建及β-葡聚糖含量QTL的定位

    Institute of Scientific and Technical Information of China (English)

    吴斌; 张茜; 宋高原; 陈新; 张宗文

    2014-01-01

    Objective]A molecular genetic linkage map for cultivated naked oat based on SSR markers was developed and Quantitative Trait Loci influencingβ-glucan content were identified, in order to facilitate the utilization of highβ-glucan content oat germplasm resources and provide a theoretical basis for oat molecular marker-assisted selection.[Method] Taken a segregation population of 215 F2:3 lines which originated from the cross with the highβ-glucan content local cultivar Xiayoumai was used as the male parent and improved cultivar Chi38 Youmai as the female as mapping population, a molecular genetic linkage map of oats with SSR markers was developed. The β-glucan content of segregation population was determined by the standard β-glucan measure method (AACC Method 32-23) which was published by American Association of Cereal Chemists and the QTLs for β-glucan content of oat were analyzed and identified by Composite Interval Mapping method. [Result] After detection of F2 progenies with 231 pairs of SSR primers, a total of 261 polymorphic markers were obtained. The polymorphic markers mentioned above were analyzed for their genetic linkage relationship by JoinMap 4.0 and finally the oat genetic linkage map was constructed. The map included 26 linkage group, 182 SSR markers and covers 1869.7cM of the whole genome. The average space between markers was 10.6 cM. The number of markers in each linkage group varied from 2 to 14 and the length of the linkage group varied from 10.6 to 235.1 cM. The results of the measurement of the parents and segregation population’sβ-glucan content showed thatβ-glucan content in the offspring groups presented as significant separation and continuous variation with variation coefficient of 18.72%, which indicated thatβ-glucan content traits are controlled by multiple genes of quantitative traits and the segregation population meets the requirements of QTL mapping. The SSR data were analyzed by QTL analysis software WinQTL Cart 2.5 and

  17. Hyaluronic Acid--an "Old" Molecule with "New" Functions: Biosynthesis and Depolymerization of Hyaluronic Acid in Bacteria and Vertebrate Tissues Including during Carcinogenesis.

    Science.gov (United States)

    Tsepilov, R N; Beloded, A V

    2015-09-01

    Hyaluronic acid is an evolutionarily ancient molecule commonly found in vertebrate tissues and capsules of some bacteria. Here we review modern data regarding structure, properties, and biological functions of hyaluronic acid in mammals and Streptococcus spp. bacteria. Various aspects of biogenesis and degradation of hyaluronic acid are discussed, biosynthesis and degradation metabolic pathways for glycosaminoglycan together with involved enzymes are described, and vertebrate and bacterial hyaluronan synthase genes are characterized. Special attention is given to the mechanisms underlying the biological action of hyaluronic acid as well as the interaction between polysaccharide and various proteins. In addition, all known signaling pathways involving hyaluronic acid are outlined. Impaired hyaluronic acid metabolism, changes in biopolymer molecular weight, hyaluronidase activity, and enzyme isoforms often accompany carcinogenesis. The interaction between cells and hyaluronic acid from extracellular matrix that may be important during malignant change is discussed. An expected role for high molecular weight hyaluronic acid in resistance of naked mole rat to oncologic diseases and the protective role of hyaluronic acid in bacteria are discussed.

  18. High-resolution imaging of selenium in kidneys: a localized selenium pool associated with glutathione peroxidase 3

    Energy Technology Data Exchange (ETDEWEB)

    Malinouski, M.; Kehr, S.; Finney, L.; Vogt, S.; Carlson, B.A.; Seravalli, J.; Jin, R.; Handy, D.E.; Park, T.J.; Loscalzo, J.; Hatfield, D.L.; Gladyshev, V.N. (Harvard-Med)

    2012-04-17

    Recent advances in quantitative methods and sensitive imaging techniques of trace elements provide opportunities to uncover and explain their biological roles. In particular, the distribution of selenium in tissues and cells under both physiological and pathological conditions remains unknown. In this work, we applied high-resolution synchrotron X-ray fluorescence microscopy (XFM) to map selenium distribution in mouse liver and kidney. Liver showed a uniform selenium distribution that was dependent on selenocysteine tRNA{sup [Ser]Sec} and dietary selenium. In contrast, kidney selenium had both uniformly distributed and highly localized components, the latter visualized as thin circular structures surrounding proximal tubules. Other parts of the kidney, such as glomeruli and distal tubules, only manifested the uniformly distributed selenium pattern that co-localized with sulfur. We found that proximal tubule selenium localized to the basement membrane. It was preserved in Selenoprotein P knockout mice, but was completely eliminated in glutathione peroxidase 3 (GPx3) knockout mice, indicating that this selenium represented GPx3. We further imaged kidneys of another model organism, the naked mole rat, which showed a diminished uniformly distributed selenium pool, but preserved the circular proximal tubule signal. We applied XFM to image selenium in mammalian tissues and identified a highly localized pool of this trace element at the basement membrane of kidneys that was associated with GPx3. XFM allowed us to define and explain the tissue topography of selenium in mammalian kidneys at submicron resolution.

  19. Biology of hyaluronan: Insights from genetic disorders of hyaluronan metabolism

    Institute of Scientific and Technical Information of China (English)

    Barbara; Triggs-Raine; Marvin; R; Natowicz

    2015-01-01

    Hyaluronan is a rapidly turned over component of the vertebrate extracellular matrix. Its levels are determined, in part, by the hyaluronan synthases, HAS1, HAS2, and HAS3, and three hyaluronidases, HYAL1, HYAL2 and HYAL3. Hyaluronan binding proteins also regulate hyaluronan levels although their involvement is less well understood. To date, two genetic disorders of hyaluronan metabolism have been reported in humans: HYAL1 deficiency(Mucopolysaccharidosis IX) in four individuals with joint pathology as the predominant phenotypic finding and HAS2 deficiency in a single person having cardiac pathology. However, inherited disorders and induced mutations affecting hyaluronan metabolism have been characterized in other species. Overproduction of hyaluronan by HAS2 results in skin folding and thickening in shar-pei dogs and the naked mole rat, whereas a complete deficiency of HAS2 causes embryonic lethality in mice due to cardiac defects. Deficiencies of murine HAS1 and HAS3 result in a predisposition to seizures. Like humans, mice with HYAL1 deficiency exhibit joint pathology. Mice lacking HYAL2 have variably penetrant developmental defects, including skeletal and cardiac anomalies. Thus, based on mutant animal models, a partial deficiency of HAS2 or HYAL2 might be compatible with survival in humans, while complete deficiencies of HAS1, HAS3, and HYAL3 may yet be recognized.

  20. The transcriptome of the bowhead whale Balaena mysticetus reveals adaptations of the longest-lived mammal

    Science.gov (United States)

    Seim, Inge; Ma, Siming; Zhou, Xuming; Gerashchenko, Maxim V.; Lee, Sang-Goo; Suydam, Robert; George, John C.; Bickham, John W.; Gladyshev, Vadim N.

    2014-01-01

    Mammals vary dramatically in lifespan, by at least two-orders of magnitude, but the molecular basis for this difference remains largely unknown. The bowhead whale Balaena mysticetus is the longest-lived mammal known, with an estimated maximal lifespan in excess of two hundred years. It is also one of the two largest animals and the most cold-adapted baleen whale species. Here, we report the first genome-wide gene expression analyses of the bowhead whale, based on the de novo assembly of its transcriptome. Bowhead whale or cetacean-specific changes in gene expression were identified in the liver, kidney and heart, and complemented with analyses of positively selected genes. Changes associated with altered insulin signaling and other gene expression patterns could help explain the remarkable longevity of bowhead whales as well as their adaptation to a lipid-rich diet. The data also reveal parallels in candidate longevity adaptations of the bowhead whale, naked mole rat and Brandt's bat. The bowhead whale transcriptome is a valuable resource for the study of this remarkable animal, including the evolution of longevity and its important correlates such as resistance to cancer and other diseases. PMID:25411232

  1. The First International Mini-Symposium on Methionine Restriction and Lifespan

    Directory of Open Access Journals (Sweden)

    Gene eAbles

    2014-05-01

    Full Text Available It has been 20 years since the Orentreich Foundation for the Advancement of Science, under the leadership Dr. Norman Orentreich, first reported that low methionine (Met ingestion by rats extends lifespan [1]. Since then, several studies have replicated the effects of dietary methionine restriction (MR in delaying age-related diseases [2–5]. We report the abstracts from the First International Mini-Symposium on Methionine Restriction and Lifespan held in Tarrytown, NY last September 2013. The goals were 1 to gather researchers with an interest in methionine restriction and lifespan, 2 to exchange knowledge, 3 to generate ideas for future investigations, and 4 to strengthen relationships within this community. The presentations highlighted the importance of research on cysteine, growth hormone (GH, and ATF4 in the paradigm of aging. In addition, the effects of dietary restriction or MR in the kidneys, liver, bones and the adipose tissue were discussed. The symposium also emphasized the value of other species, e.g. the naked mole rat, Brandt’s bat and drosophila in aging research. Overall, the symposium consolidated scientists with similar research interests and provided opportunities to conduct future collaborative studies.

  2. The transcriptome of the bowhead whale Balaena mysticetus reveals adaptations of the longest-lived mammal.

    Science.gov (United States)

    Seim, Inge; Ma, Siming; Zhou, Xuming; Gerashchenko, Maxim V; Lee, Sang-Goo; Suydam, Robert; George, John C; Bickham, John W; Gladyshev, Vadim N

    2014-10-01

    Mammals vary dramatically in lifespan, by at least two-orders of magnitude, but the molecular basis for this difference remains largely unknown. The bowhead whale Balaena mysticetus is the longest-lived mammal known, with an estimated maximal lifespan in excess of two hundred years. It is also one of the two largest animals and the most cold-adapted baleen whale species. Here, we report the first genome-wide gene expression analyses of the bowhead whale, based on the de novo assembly of its transcriptome. Bowhead whale or cetacean-specific changes in gene expression were identified in the liver, kidney and heart, and complemented with analyses of positively selected genes. Changes associated with altered insulin signaling and other gene expression patterns could help explain the remarkable longevity of bowhead whales as well as their adaptation to a lipid-rich diet. The data also reveal parallels in candidate longevity adaptations of the bowhead whale, naked mole rat and Brandt's bat. The bowhead whale transcriptome is a valuable resource for the study of this remarkable animal, including the evolution of longevity and its important correlates such as resistance to cancer and other diseases.

  3. Facile SILAR approach to air-stable naked silver and gold nanoparticles supported by alumina.

    Science.gov (United States)

    Stamplecoskie, Kevin G; Manser, Joseph S

    2014-10-22

    A synthetically convenient and scalable SILAR (successive ion layer adsorption and reaction) method is used to make air-stable films of silver and gold nanoparticles supported on alumina scaffolds. This solution-based deposition technique yields particles devoid of insulating capping agents or ligands. The optical properties of the nanoparticle films were investigated using femtosecond transient absorption spectroscopy. A linear absorption arising from intraband excitation (775 nm laser pulse) is seen only for Au nanoparticles at low intensity. However, both Au and Ag particles exhibit plasmon resonance responses at high excitation intensity via two photon absorption of the 775 nm pump pulse. The difference in optical response to near-IR laser excitation is rationalized based on the known density of states for each metal. To demonstrate the potential applications of these films, alumina-supported Ag nanoparticles were utilized as substrates for surface enhanced Raman spectroscopy, resulting in a 65-fold enhancement in the Raman signal of the probe molecule rhodamine 6G. The exceptional stability and scalability of these SILAR films opens the door for further optical and photocatalytic studies and applications, particularly with ligand-free Ag nanoparticles that typically oxidize under ambient conditions. Additionally, isolating plasmonic and interband electronic excitations in stable AgNP under visible light irradiation could enable elucidation of the mechanisms that drive noble metal-assisted photocatalytic processes.

  4. "Sometimes the naked taste of potato reminds me of being poor".

    Science.gov (United States)

    Potter, Polyxeni

    2009-06-01

    "I want to paint men and women with that something of the eternal which the halo used to symbolize and which we seek to convey by the actual radiance and vibration of our coloring," wrote Vincent van Gogh in one of his celebrated letters. Hundreds of these were written mostly to his brother Theo, an art dealer in Paris who provided him with financial and emotional support throughout his brief but brilliant career. The letters lay out the artist's philosophy of life and reveal ample literary inclinations as well as spiritual depth. "Saying a thing well is as interesting and as difficult as painting it," he wrote.

  5. Dressing a naked quasar: star formation and AGN feedback in HE0450-2958

    CERN Document Server

    Klamer, I; Ekers, R; Middelberg, E; Klamer, Ilana; Papadopoulos, Padelis; Ekers, Ron; Middelberg, Enno

    2007-01-01

    We present Australia Telescope Compact Array radio continuum observations of the quasar/galaxy system HE0450-2958. An asymetric triple linear morphology is observed, with the central radio component coincident with the quasar core and a second radio component associated with a companion galaxy at a projected distance of 7kpc from the quasar. The system obeys the far-infrared to radio continuum correlation, implying the radio emission is energetically dominated by star formation activity. However, there is undoubtedly some contribution to the overall radio emission from a low-luminosity AGN core and a pair of radio lobes. Long baseline radio interferometric observations of the quasar core place a 3sigma upper limit of 0.6mJy at 1400MHz on the AGN contribution to the quasar's radio emission; less than 30% of the total. The remaining 70% of the radio emission from the quasar is associated with star formation activity and provides the first direct evidence for the quasar's host galaxy. A re-anlaysis of the VLT sp...

  6. A Politics of Nudity: Photographs of the 'Naked Mru' of Bangladesh

    NARCIS (Netherlands)

    van Schendel, W.

    2002-01-01

    This article uses photographs to explore the meanings of nudity in a district of Bangladesh. Throughout the colonial and postcolonial periods, photography was a major tool here in the framing of a confrontation between local and external cultural styles. In this confrontation, nudity was used as a

  7. Species of Naked Amoebae (Protista new for the Fauna of Ukraine

    Directory of Open Access Journals (Sweden)

    Patsyuk M. K.

    2015-10-01

    Full Text Available Представлены сведения об обнаружении новых для фауны Украины голых амеб: Rhizamoeba sp., Thecamoeba quadrilineata (Carter, 1856, Thecamoeba verrucosa (Ehrenberg, 1838, Flamella sp., Penardia mutabilis Cash, 1904

  8. Design of a gold nanoprobe for rapid and portable mercury detection with the naked eye.

    Science.gov (United States)

    He, Shijiang; Li, Di; Zhu, Changfeng; Song, Shiping; Wang, Lihua; Long, Yitao; Fan, Chunhai

    2008-10-28

    A gold nanoprobe that can respond colorimetrically to Hg(2+) is designed and coupled with a power-free PDMS device; the system can be used for rapid and visual detection of low micromolar Hg(2+) in real environmental samples.

  9. When Naked Became Armored: An Eight-Gene Phylogeny Reveals Monophyletic Origin of Theca in Dinoflagellates

    Science.gov (United States)

    Orr, Russell J. S.; Murray, Shauna A.; Stüken, Anke; Rhodes, Lesley; Jakobsen, Kjetill S.

    2012-01-01

    The dinoflagellates are a diverse lineage of microbial eukaryotes. Dinoflagellate monophyly and their position within the group Alveolata are well established. However, phylogenetic relationships between dinoflagellate orders remain unresolved. To date, only a limited number of dinoflagellate studies have used a broad taxon sample with more than two concatenated markers. This lack of resolution makes it difficult to determine the evolution of major phenotypic characters such as morphological features or toxin production e.g. saxitoxin. Here we present an improved dinoflagellate phylogeny, based on eight genes, with the broadest taxon sampling to date. Fifty-five sequences for eight phylogenetic markers from nuclear and mitochondrial regions were amplified from 13 species, four orders, and concatenated phylogenetic inferences were conducted with orthologous sequences. Phylogenetic resolution is increased with addition of support for the deepest branches, though can be improved yet further. We show for the first time that the characteristic dinoflagellate thecal plates, cellulosic material that is present within the sub-cuticular alveoli, appears to have had a single origin. In addition, the monophyly of most dinoflagellate orders is confirmed: the Dinophysiales, the Gonyaulacales, the Prorocentrales, the Suessiales, and the Syndiniales. Our improved phylogeny, along with results of PCR to detect the sxtA gene in various lineages, allows us to suggest that this gene was probably acquired separately in Gymnodinium and the common ancestor of Alexandrium and Pyrodinium and subsequently lost in some descendent species of Alexandrium. PMID:23185516

  10. Silencing honey bee naked cuticle (nkd) reduces Nosema ceranae replication and disease levels

    Science.gov (United States)

    Nosema ceranae is a new and emerging microsporidian parasite of European honey bees, Apis mellifera that has been implicated in alarming colony losses worldwide. RNA interference (RNAi), a post-transcriptional gene silencing mechanism, has emerged as a potent and specific strategy for controlling in...

  11. A ratiometric chemodosimeter for highly selective naked-eye and fluorogenic detection of cyanide.

    Science.gov (United States)

    Lin, Wei-Chi; Hu, Jiun-Wei; Chen, Kew-Yu

    2015-09-17

    A simple indole-based chemosensor (1) with a very low molecular weight of 207 g mol(-1) has been synthesized for the highly reactive and selective detection of CN(-) in aqueous media, even in the presence of other anions, such as F(-), Cl(-), Br(-), AcO(-), [Formula: see text] , SCN(-), [Formula: see text] , [Formula: see text] , [Formula: see text] , BzO(-), [Formula: see text] , and [Formula: see text] . The sensor achieves rapid detection of cyanide anion in 2 min, and the pseudo-first-order rate constant is estimated as 1.576 min(-1). The colorimetric and ratiometric fluorescent response of the sensor to CN(-) is attributable to the addition of CN(-) to the electron-deficient dicyanovinyl group of 1, which prevents intramolecular charge transfer. The sensing mechanism is supported by density functional theory and time-dependent density functional theory calculations. Moreover, sensor 1 exhibits both high accuracy in determining the concentration of CN(-) in real samples and 1-based test strips can conveniently detect CN(-) without any additional equipment. The detection limit of the sensor 1 (1.1 μM) for cyanide is lower than the maximum permissible level of CN(-) (1.9 μM) in drinking water. Copyright © 2015 Elsevier B.V. All rights reserved.

  12. Cervical cancer screening with naked-eye visual inspection in Colombia.

    Science.gov (United States)

    Murillo, Raul; Luna, Joaquin; Gamboa, Oscar; Osorio, Elkin; Bonilla, Jairo; Cendales, Ricardo

    2010-06-01

    To assess the accuracy of visual inspection provided by nurses through combining acetic acid (VIA) and Lugol's iodine (VILI) in a low-resource region of Colombia. A cross-sectional study with 4957 women was conducted to evaluate visual inspection techniques as the basis for see-and-treat approaches in cervical cancer control. All women underwent conventional cytology, VIA performed by nurses, and a combination of VIA and VILI. All women underwent colposcopy and biopsies were obtained for any positive test. A total of 762 women underwent biopsy, 4945 women were included in the analysis of conventional cytology, and 4957 were included in the analysis of VIA and VIA-VILI. Positivity rates were 1.3% and 4.3% for HSIL and LSIL cytology, 7.4% for VIA, and 10.1% for VIA-VILI. Sensitivity for cytology was 52.9% and 36.8% for LSIL and HSIL thresholds, 53.6% for VIA, and 68.1% for VIA-VILI. The corresponding specificity was 95.0%, 99.2%, 93.2%, and 90.8% respectively. The parallel combination of VIA-VILI and cytology LSIL-threshold revealed the best performance as a screening strategy. The use of VIA-VILI simulating colposcopic procedures and provided by nurses represents a good alternative for implementing see-and-treat programs in Latin America. Program constraints should be taken into account. Copyright 2010 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

  13. Static and Infalling Quasilocal Energy of Charged and Naked Black Holes

    OpenAIRE

    Booth, I. S.; Mann, R. B.

    1999-01-01

    We extend the quasilocal formalism of Brown and York to include electromagnetic and dilaton fields and also allow for spatial boundaries that are not orthogonal to the foliation of the spacetime. The extension allows us to study the quasilocal energy measured by observers who are moving around in a spacetime. We show that the quasilocal energy transforms with respect to boosts by Lorentz-type transformation laws. The resulting formalism can be used to study spacetimes containing electric or m...

  14. Characterisation of colloidal drug delivery systems from the naked eye to Cryo-FESEM

    DEFF Research Database (Denmark)

    Krauel, Karen; Girvan, Liz; Hook, Sarah;

    2007-01-01

    for the microemulsions of the two pseudoternary systems ethyloleate, polyoxyethylene 20 sorbitan mono-oleate/sorbitan monolaurate and water with and without butanol as a cosurfactant were determined by visual observation of the samples. Microemulsions containing liquid crystals were determined by polarisation light...... by conventional scanning electron microscopy. The size of the nanoparticles obtained from electron microscopy was in good agreement with particle sizing techniques (photon correlation spectroscopy) from earlier studies and no morphological differences could be observed in particles prepared from the different...... types of microemulsions. Cryo-field emission scanning electron microscopy proved to be a most valuable technique in the visualisation of the colloidal systems as samples could be observed close to their natural state....

  15. Digestible Lysine on Live Performance of Chicken Type Naked Neck During the Starter Phase

    Directory of Open Access Journals (Sweden)

    RG de Oliveira

    2015-12-01

    Full Text Available ABSTRACT The poultry market has changed due to a higher consumer interest on products with differentiated organoleptic characteristics, making of free-range broiler production a promising activity. This experiment was conducted to determine the digestible lysine requirements of Redbro Cou Nu male and female chickens during the starter phase (one to 21 days of age. Six hundred and thirty Redbro Cou Nu broilers were distributed into 30 pens (21 chickens/pen according to a randomized complete design in a 5 x 2 factorial arrangement, consisting of five levels of digestible lysine and two sexes, with three replicates (pens per treatments. Diets with increasing digestible lysine levels (8.1, 9.5, 10.9, 12.3 and 13.7 g of digestible lysine per kg of diet were offered ad libitum. The following performance traits were evaluated at the end of the experiment (d 21: feed intake, lysine intake, body weight gain, and feed conversion ratio. No interaction between dietary lysine level and sex was observed for the evaluated traits. The effect of sex was only detected on body weight gain, while effects of dietary lysine level were only detected on the feed intake. Males presented higher body weight gain than females. Lysine intake and body weight gain increased, and feed conversion ratio decreased as the level of dietary lysine increased. The best feed conversion ratio was obtained when birds were fed 12.95 g of digestible lysine per kg of diet.

  16. The naked truth about HIV and risk taking in Swedish prisons: A qualitative study.

    Science.gov (United States)

    Lindbom, Sigrid J A; Larsson, Markus; Agardh, Anette

    2017-01-01

    This qualitative study explores former prison inmates' perceptions and attitudes towards HIV risk inside Swedish prisons. In 2014, eight semi-structured interviews were conducted with former male prisoners to gain a deeper understanding of situations perceived to be associated with risk of HIV transmission. The material gathered from the interviews was analyzed by manifest and latent qualitative content analysis. The findings revealed that risky behavioral practices, such as sharing needles, unprotected sexual activity, and lack of openness about HIV status represented potential health threats with regard to the risk of HIV transmission. Evidence from the study indicates that educational interventions regarding HIV and the transmission routes are required for HIV prevention in Swedish prisons.

  17. Investigation of Electrochemical Charging Behaviors of "Naked" Gold Nanoparticles Ensembles in Aqueous Media

    Institute of Scientific and Technical Information of China (English)

    CHENG,Wen-Long(程文龙); HAN,Xiao-Jun(韩晓军); PENG,Zhang-Quan(彭章泉); DOGN,Shao-Jun(董绍俊); WANG,Er-Kang(汪尔康)

    2002-01-01

    Gold nanoparticles were immobilized onto the electrode surface by simple self-assembly technique. Interestingly, the ensmbles of these nanoparticles exhibit quantized charging behaviors in aqueous solution. Possible mechanism for such behaviors was proposed.

  18. Naked DNA Immunization for Prevention of Prostate Cancer in a Dunning Rat Prostate Tumor Model

    Science.gov (United States)

    2005-06-01

    when primed with the tPSA DCs) were CD3+CD8+ (data not shown). Selection of HLA-A2-binding PSA- and PAP- peptides For the present study, the aminoacid ...sequence of the truncated (no-leader sequence) PSA (AA 25-161) and PAP (AA 33-386) were analyzed for the existence of 9- aminoacid peptides predicted...identical aminoacid sequences. Due to post-translational modification in the ER, however, the secreted protein is N- glycosylated and secreted, while the

  19. Naked DNA Immunization of Prevention of Prostate Cancer in a Dunning Rat Prostate Tumor Model

    Science.gov (United States)

    2006-06-01

    PSMA peptides The aminoacid sequence of the extracellular domain of PSMA was analyzed for the existence of 9- aminoacid peptides predicted to bind...CD3+CD8+ (data not shown). Selection of HLA-A2-binding PSA- and PAP- peptides For the present study, the aminoacid sequence of the truncated (no...leader sequence) PSA (AA 25-161) and PAP (AA 33-386) were analyzed for the existence of 9- aminoacid peptides predicted to bind to HLA-A201, the most

  20. The naked planet Earth: Most essential pre-requisite for the origin and evolution of life

    Directory of Open Access Journals (Sweden)

    S. Maruyama

    2013-03-01

    To satisfy the tight conditions to make the Earth habitable, the formation mechanism of primordial Earth is an important factor. At first, a ‘dry Earth’ must be made through giant impact, followed by magma ocean to float nutrient-enriched primordial continents (anorthosite + KREEP. Late bombardment from asteroid belt supplied water to make 3–5 km thick ocean, and not from icy meteorites from Kuiper belt beyond cool Jupiter. It was essential to meet the above conditions that enabled the Earth as a habitable planet with evolved life forms. The tight constraints that we evaluate for birth and evolution of life on Earth would provide important guidelines for planetary scientists hunting for life in the exo-solar planets.

  1. Bare Quark Stars or Naked Neutron Stars: The Case of RX J1856.5-3754

    CERN Document Server

    Turolla, R; Drake, J J; Turolla, Roberto; Zane, Silvia; Drake, Jeremy J.

    2004-01-01

    In a cool neutron star (T 10^13 G), a phase transition may occur in the outermost layers. As a consequence the neutron star becomes `bare', i.e. no gaseous atmosphere sits on the top of the crust. The surface of cooling, bare neutron stars not necessary gives off blackbody radiation because of the strong suppression in the emissivity at energies below the electron plasma frequency \\omega_p. Since \\omega_p~1 keV under the conditions typical of the dense electron gas in the condensate, the emission from a T~100 eV bare neutron star will be substantially depressed with respect to that of a perfect Planckian radiator at most energies. Here we present a detailed analysis of the emission properties of a bare neutron star. In particular, we derive the surface emissivity for a Fe composition in a range of magnetic fields and temperatures representative of cooling isolated neutron stars, like RX J1856.5-3754. We find that the emitted spectrum is strongly dependent on the electron conductivity in the solid surface lay...

  2. Revealing the naked truth behind price determinacy, infinite-horizon rational expectations, and inflation targeting

    OpenAIRE

    Eagle, David

    2007-01-01

    The economic profession should demand that that price-determinacy literature adhere to normal academic standards and burdens of proof. By presenting two examples where the non-exploding criterion fails miserably, we demonstrate that that criterion does not universally apply. Therefore, the previous price-determinacy literature has the burden to prove that the non-explosive criterion does apply, but has not met and probably cannot meet that burden. This paper looks at an economy with an arb...

  3. Axially Symmetric Null Dust Space-Time, Naked Singularity, and Cosmic Time Machine

    National Research Council Canada - National Science Library

    Faizuddin Ahmed

    2017-01-01

    ... the cylinder which has closed orbits. The space-time admits closed timelike curves (CTCs) which develop at some particular moment in a causally well-behaved manner and may represent a Cosmic Time Machine...

  4. The marketing of dietary supplements in North America: the emperor is (almost) naked.

    Science.gov (United States)

    Temple, Norman J

    2010-07-01

    Many different dietary supplements are being sold in North America. The quality of the evidence supporting their efficacy covers a wide spectrum: Some are based on solid science (such as vitamin D and fish oil), whereas with most supplements there is little or no supporting evidence. Types of supplements commonly sold include exotic fruit juices (such as goji juice) and single herbs or mixture of herbs. Common claims made in support of particular supplements are that they are rich in antioxidants, induce detoxification, stimulate the immune system, and cause weight loss. Supplements are commonly sold through health food stores and by multilevel marketing. Sales may be promoted using bulk mail ("junk mail"), spam e-mails, and Web sites. A large part of marketing is based on claims that are blatantly dishonest. Whereas supplements for which good supporting evidence exists generally cost around $3-$4 per month, those that are heavily promoted for which there is little supporting evidence cost about $20-$60 per month. The major cause of this problem in the United States is weakness of the law. There is an urgent need for stricter regulation and for giving better advice to the general public.

  5. Biodistribution and acute toxicity of naked gold nanoparticles in a rabbit hepatic tumor model.

    Science.gov (United States)

    Glazer, Evan S; Zhu, Cihui; Hamir, Amir N; Borne, Agatha; Thompson, Catherine Shea; Curley, Steven A

    2011-12-01

    There is a paucity of data regarding the safety of administering solid gold nanoparticles (AuNPs) in large animal tumor models. We assessed the acute toxicity and biodistribution of 5 nm and 25 nm solid AuNPs in New Zealand White rabbits (n = 6 in each) with implanted liver Vx2 tumors 24 h after intravenous injection. Gold concentration was determined by inductively coupled plasma atomic emission spectrometry (ICP) and imaged with transmission electron microscopy (TEM). There was no clinico-pathologic evidence of renal, hepatic, pulmonary, or other organ dysfunction. After 25 nm AuNP administration, the concentration of white blood cells increased after treatment (p = 0.001). Most other blood studies were unchanged. AuNPs were distributed to the spleen, liver, and Vx2 tumors, but not to other tissues. The urinary excretion of AuNPs was bimodal as measured by ICP. 25 nm AuNPs were more evenly distributed throughout tissues and may be better tools for medical therapy.

  6. An alternative well-posedness property and static spacetimes with naked singularities

    Energy Technology Data Exchange (ETDEWEB)

    Gamboa Saravi, Ricardo E [Departamento de Fisica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 67, 1900 La Plata (Argentina); Sanmartino, Marcela [Departamento de Matematica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Casilla de Correo 172, 1900 La Plata (Argentina); Tchamitchian, Philippe, E-mail: quique@Fisica.unlp.edu.a, E-mail: tatu@mate.unlp.edu.a, E-mail: philippe.tchamitchian@univ-cezanne.f [Universite Paul Cezanne, CNRS, LATP (UMR 6632), Faculte des Sciences et Techniques, LATP, Case cour A, Avenue Escadrille Normandie-Niemen, F-13397 Marseille Cedex 20 (France)

    2010-11-07

    In the first part of this paper, we show that the Cauchy problem for wave propagation in some static spacetimes presenting a singular timelike boundary is well-posed, if we only require the waves to have finite energy, although no boundary condition is required. This feature does not come from essential self-adjointness, which is false in these cases, but from a different phenomenon that we call the alternative well-posedness property, whose origin is due to the degeneracy of the metric components near the boundary. Beyond these examples, in the second part, we characterize the type of degeneracy which leads to this phenomenon.

  7. MICROSPECTROPHOTOMETRY AS A METHOD TO IDENTIFY KLEPTOPLASTIDS IN THE NAKED FRESHWATER DINOFLAGELLATE GYMNODINIUM ACIDOTUM(1).

    Science.gov (United States)

    Barsanti, Laura; Evangelista, Valtere; Passarelli, Vincenzo; Frassanito, Anna Maria; Coltelli, Primo; Gualtieri, Paolo

    2009-12-01

    A relatively small number of freshwater dinoflagellates are involved in symbiotic association with cryptophytes. The chloroplasts of the cryptophytes are retained by the dinoflagellate and give it the characteristic phycobilin pigmentation, either phycoerythrin or phycocyanin. The pigment characterization of the retained chloroplasts can give precise and accurate information about the type of cryptophyte preyed upon by the dinoflagellate. For this purpose, we performed microspectrophotometric evaluation of the pigments of Gymnodinium acidotum Nygaard and three different cryptophytes present in samples collected from a tributary of the river Arno, in Tuscany (Italy). The comparison of the different spectroscopic data allowed us to discriminate effectively among the cryptophytes preyed upon by the dinoflagellate.

  8. Direct Oxidative Damage of Naked DNA Generated upon Absorption of UV Radiation by Nucleobases.

    Science.gov (United States)

    Gomez-Mendoza, Miguel; Banyasz, Akos; Douki, Thierry; Markovitsi, Dimitra; Ravanat, Jean-Luc

    2016-10-06

    It has been shown that in addition to formation of pyrimidine dimers, UV irradiation of DNA in the absence of photosensitizer also induces formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine, but the mechanism of formation of that oxidized base has not been clearly established. In the present study, we provide an unambiguous demonstration that absorption of UVC and UVB radiation by the nucleobases induces DNA oxidation via a direct process (one-electron oxidation) and not singlet oxygen. Evidence arose from the fact that polyamine-guanine adducts that are specifically produced through the transient formation of guanine radical cation are generated following UV irradiation of DNA in the presence of a polyamine even in the absence of any photosensitizer.

  9. Remote stereoscopic video play platform for naked eyes based on the Android system

    Science.gov (United States)

    Jia, Changxin; Sang, Xinzhu; Liu, Jing; Cheng, Mingsheng

    2014-11-01

    As people's life quality have been improved significantly, the traditional 2D video technology can not meet people's urgent desire for a better video quality, which leads to the rapid development of 3D video technology. Simultaneously people want to watch 3D video in portable devices,. For achieving the above purpose, we set up a remote stereoscopic video play platform. The platform consists of a server and clients. The server is used for transmission of different formats of video and the client is responsible for receiving remote video for the next decoding and pixel restructuring. We utilize and improve Live555 as video transmission server. Live555 is a cross-platform open source project which provides solutions for streaming media such as RTSP protocol and supports transmission of multiple video formats. At the receiving end, we use our laboratory own player. The player for Android, which is with all the basic functions as the ordinary players do and able to play normal 2D video, is the basic structure for redevelopment. Also RTSP is implemented into this structure for telecommunication. In order to achieve stereoscopic display, we need to make pixel rearrangement in this player's decoding part. The decoding part is the local code which JNI interface calls so that we can extract video frames more effectively. The video formats that we process are left and right, up and down and nine grids. In the design and development, a large number of key technologies from Android application development have been employed, including a variety of wireless transmission, pixel restructuring and JNI call. By employing these key technologies, the design plan has been finally completed. After some updates and optimizations, the video player can play remote 3D video well anytime and anywhere and meet people's requirement.

  10. Ultrastructural and Cytochemical Properties of Peripheral Blood Cells of Piebald Naked Carp (Gymnocypris eckloni).

    Science.gov (United States)

    Zheng, Z X; Tang, Y; Fang, J; Peng, X; Fan, J D; Cui, H M; Yang, L Z

    2017-02-01

    The ultrastructural and cytochemical properties of peripheral blood cells of Gymnocypris eckloni were investigated by transmission electron microscopy and a range of cytochemical techniques to provide clear insight into the structure and function of blood cells from this fish. Ultrastructurally, erythrocytes, leucocytes (neutrophils, eosinophils, lymphocytes, monocytes), thrombocytes and plasma cells were identified in the peripheral blood of G. eckloni. The most special ultrastructural characteristics of blood cells in this fish were that neutrophils exhibited only one type of cytoplasmic granules containing an eccentric, spherical or oval electron-dense core, and eosinophils presented two types of granules with non-uniform electronic density and without crystalloids in their cytoplasm. Neutrophils, eosinophils, lymphocytes, monocytes and thrombocytes were positive for periodic acid-Schiff and α-naphthyl acetate esterase staining. Intense peroxidase positive staining was observed in neutrophils and monocytes, but not in eosinophils, lymphocytes and thrombocytes. Neutrophils, eosinophils and monocytes were stained positively for acid phosphatase, whereas lymphocytes and thrombocytes did not stain. Leucocytes and thrombocytes were negative for alkaline phosphatase and Sudan black B staining. Erythrocytes were negative for all cytochemical staining. The cytochemical and ultrastructural features of peripheral blood cells of G. eckloni were similar to those of other fish species. However, some important differences were identified in G. eckloni.

  11. Recent advances in design of immunogenic and effective naked DNA vaccines against cancer.

    Science.gov (United States)

    Fioretti, Daniela; Iurescia, Sandra; Rinaldi, Monica

    2014-01-01

    A variety of clinical trials for vaccines against cancer have provided evidence that DNA vaccines are well tolerated and have an excellent safety profile. DNA vaccines require much improvement to make them sufficiently effective against cancer in the clinic. Nowadays, it is clear that an increased antigen expression correlates with improved immunogenicity and it is critical to vaccine performance in large animals and humans. Similarly, additional strategies are required to activate effective immunity against poorly immunogenic tumour antigens. This review discusses very recent scientific references focused on the development of sophisticated DNA vaccines against cancer. We report a selection of novel and relevant patents employed to improve their immunogenicity through several strategies such as the use of tissue-specific transcriptional elements, nuclear localisation signalling, codon-optimisation and by targeting antigenic proteins to secretory pathway. Recent patents validating portions or splice variants of tumour antigens as candidates for cancer DNA vaccines with improved specificity, such as mesothelin and hTERT, are also discussed. Lastly, we review novel patents on the use of genetic immunomodulators, such as "universal" T helper epitopes derived from tetanus toxin, E. coli heat labile enterotoxin and vegetable proteins, as well as cytokines, chemokines or costimulatory molecules such as IL-6, IL-15, IL- 21 to amplify immunity against cancer.

  12. When naked became armored: an eight-gene phylogeny reveals monophyletic origin of theca in dinoflagellates.

    Science.gov (United States)

    Orr, Russell J S; Murray, Shauna A; Stüken, Anke; Rhodes, Lesley; Jakobsen, Kjetill S

    2012-01-01

    The dinoflagellates are a diverse lineage of microbial eukaryotes. Dinoflagellate monophyly and their position within the group Alveolata are well established. However, phylogenetic relationships between dinoflagellate orders remain unresolved. To date, only a limited number of dinoflagellate studies have used a broad taxon sample with more than two concatenated markers. This lack of resolution makes it difficult to determine the evolution of major phenotypic characters such as morphological features or toxin production e.g. saxitoxin. Here we present an improved dinoflagellate phylogeny, based on eight genes, with the broadest taxon sampling to date. Fifty-five sequences for eight phylogenetic markers from nuclear and mitochondrial regions were amplified from 13 species, four orders, and concatenated phylogenetic inferences were conducted with orthologous sequences. Phylogenetic resolution is increased with addition of support for the deepest branches, though can be improved yet further. We show for the first time that the characteristic dinoflagellate thecal plates, cellulosic material that is present within the sub-cuticular alveoli, appears to have had a single origin. In addition, the monophyly of most dinoflagellate orders is confirmed: the Dinophysiales, the Gonyaulacales, the Prorocentrales, the Suessiales, and the Syndiniales. Our improved phylogeny, along with results of PCR to detect the sxtA gene in various lineages, allows us to suggest that this gene was probably acquired separately in Gymnodinium and the common ancestor of Alexandrium and Pyrodinium and subsequently lost in some descendent species of Alexandrium.

  13. Highly dynamic coordination behavior of Pn ligand complexes towards "naked" Cu(+) cations.

    Science.gov (United States)

    Fleischmann, Martin; Welsch, Stefan; Peresypkina, Eugenia V; Virovets, Alexander V; Scheer, Manfred

    2015-10-01

    Reactions of Cu(+) containing the weakly coordinating anion [Al{OC(CF3 )3 }4 ](-) with the polyphosphorus complexes [{CpMo(CO)2 }2 (μ,η(2) :η(2) -P2 )] (A), [CpM(CO)2 (η(3) -P3 )] (M=Cr(B1), Mo (B2)), and [Cp*Fe(η(5) -P5 )] (C) are presented. The X-ray structures of the products revealed mononuclear (4) and dinuclear (1, 2, 3) Cu(I) complexes, as well as the one-dimensional coordination polymer (5 a) containing an unprecedented [Cu2 (C)3 ](2+) paddle-wheel building block. All products are readily soluble in CH2 Cl2 and exhibit fast dynamic coordination behavior in solution indicated by variable temperature (31) P{(1) H} NMR spectroscopy.

  14. Genome based analyses of six hexacorallian species reject the “naked coral” hypothesis

    KAUST Repository

    Wang, Xin

    2017-09-23

    Scleractinian corals are the foundation species of the coral-reef ecosystem. Their calcium carbonate skeletons form extensive structures that are home to millions of species, making coral reefs one of the most diverse ecosystems of our planet. However, our understanding of how reef-building corals have evolved the ability to calcify and become the ecosystem builders they are today is hampered by uncertain relationships within their subclass Hexacorallia. Corallimorpharians have been proposed to originate from a complex scleractinian ancestor that lost the ability to calcify in response to increasing ocean acidification, suggesting the possibility for corals to lose and gain the ability to calcify in response to increasing ocean acidification. Here we employed a phylogenomic approach using whole-genome data from six hexacorallian species to resolve the evolutionary relationship between reef-building corals and their non-calcifying relatives. Phylogenetic analysis based on 1,421 single-copy orthologs, as well as gene presence/absence and synteny information, converged on the same topologies, showing strong support for scleractinian monophyly and a corallimorpharian sister clade. Our broad phylogenomic approach using sequence-based and sequence-independent analyses provides unambiguous evidence for the monophyly of scleractinian corals and the rejection of corallimorpharians as descendants of a complex coral ancestor.

  15. In vivo evaluation of the biodistribution of intravenously administered naked and functionalised silver nanoparticles in rabbit

    KAUST Repository

    Ashraf, Ayesha

    2015-12-01

    Water-based suspension of silver nanoparticles (AgNPs) and dextran coated AgNPs (dextran-AgNPs) are fabricated and characterised for intravenous administration. A simple method for radiolabelling of nanoparticles with 99mTc was used. Labelling efficiency for AgNPs and dextran-AgNPs was found to be more than 80 and 88%, respectively. In vivo tissue uptake of nanoparticles during dynamic phase, after systematic administration by biodistribution analysis with single-photon emission computed tomography imaging has been evaluated. Biodistribution analysis revealed that 99mTc-AgNPs and 99mTc-dextran-AgNPs are mainly accumulated in liver/spleen region but 99mTc-dextran-AgNPs delayed recognition and uptake by liver. Results indicate that dextran-AgNPs are able to evade reticuloendothelum system with enhanced blood retention time. Accumulation of nanoparticles in liver/spleen region implicates the utilisation of AgNPs for liver cancer treatment. © 2015 The Institution of Engineering and Technology.

  16. Adaptive evolution of voltage-gated sodium channels: the first 800 million years.

    Science.gov (United States)

    Zakon, Harold H

    2012-06-26

    Voltage-gated Na(+)-permeable (Nav) channels form the basis for electrical excitability in animals. Nav channels evolved from Ca(2+) channels and were present in the common ancestor of choanoflagellates and animals, although this channel was likely permeable to both Na(+) and Ca(2+). Thus, like many other neuronal channels and receptors, Nav channels predated neurons. Invertebrates possess two Nav channels (Nav1 and Nav2), whereas vertebrate Nav channels are of the Nav1 family. Approximately 500 Mya in early chordates Nav channels evolved a motif that allowed them to cluster at axon initial segments, 50 million years later with the evolution of myelin, Nav channels "capitalized" on this property and clustered at nodes of Ranvier. The enhancement of conduction velocity along with the evolution of jaws likely made early gnathostomes fierce predators and the dominant vertebrates in the ocean. Later in vertebrate evolution, the Nav channel gene family expanded in parallel in tetrapods and teleosts (∼9 to 10 genes in amniotes, 8 in teleosts). This expansion occurred during or after the late Devonian extinction, when teleosts and tetrapods each diversified in their respective habitats, and coincided with an increase in the number of telencephalic nuclei in both groups. The expansion of Nav channels may have allowed for more sophisticated neural computation and tailoring of Nav channel kinetics with potassium channel kinetics to enhance energy savings. Nav channels show adaptive sequence evolution for increasing diversity in communication signals (electric fish), in protection against lethal Nav channel toxins (snakes, newts, pufferfish, insects), and in specialized habitats (naked mole rats).

  17. Does the oxidative stress theory of aging explain longevity differences in birds? II. Antioxidant systems and oxidative damage.

    Science.gov (United States)

    Montgomery, Magdalene K; Buttemer, William A; Hulbert, A J

    2012-03-01

    The oxidative damage hypothesis of aging posits that the accumulation of oxidative damage is a determinant of an animal species' maximum lifespan potential (MLSP). Recent findings in extremely long-living mammal species such as naked mole-rats challenge this proposition. Among birds, parrots are exceptionally long-living with an average MLSP of 25 years, and with some species living more than 70 years. By contrast, quail are among the shortest living bird species, averaging about 5-fold lower MLSP than parrots. To test if parrots have correspondingly (i) superior antioxidant protection and (ii) lower levels of oxidative damage compared to similar-sized quail, we measured (i) total antioxidant capacity, uric acid and reduced glutathione (GSH) levels, as well as the activities of enzymatic antioxidants (superoxide dismutase, glutathione peroxidase and catalase), and (ii) markers of mitochondrial DNA damage (8-OHdG), protein damage (protein carbonyls) and lipid peroxidation (lipid hydroperoxides and TBARS) in three species of long-living parrots and compared these results to corresponding measures in two species of short-living quails (average MLSP=5.5 years). All birds were fed the same diet to exclude differences in dietary antioxidant levels. Tissue antioxidants and oxidative damage were determined both 'per mg protein' and 'per g tissue'. Only glutathione peroxidase was consistently higher in tissues of the long-living parrots and suggests higher protection against the harmful effects of hydroperoxides, which might be important for parrot longevity. The levels of oxidative damage were mostly statistically indistinguishable between parrots and quails (67%), occasionally higher (25%), but rarely lower (8%) in the parrots. Despite indications of higher protection against some aspects of oxidative stress in the parrots, the pronounced longevity of parrots appears to be independent of their antioxidant mechanisms and their accumulation of oxidative damage. Copyright

  18. Elevated proteasome capacity extends replicative lifespan in Saccharomyces cerevisiae.

    Directory of Open Access Journals (Sweden)

    Undine Kruegel

    2011-09-01

    Full Text Available Aging is characterized by the accumulation of damaged cellular macromolecules caused by declining repair and elimination pathways. An integral component employed by cells to counter toxic protein aggregates is the conserved ubiquitin/proteasome system (UPS. Previous studies have described an age-dependent decline of proteasomal function and increased longevity correlates with sustained proteasome capacity in centenarians and in naked mole rats, a long-lived rodent. Proof for a direct impact of enhanced proteasome function on longevity, however, is still lacking. To determine the importance of proteasome function in yeast aging, we established a method to modulate UPS capacity by manipulating levels of the UPS-related transcription factor Rpn4. While cells lacking RPN4 exhibit a decreased non-adaptable proteasome pool, loss of UBR2, an ubiquitin ligase that regulates Rpn4 turnover, results in elevated Rpn4 levels, which upregulates UPS components. Increased UPS capacity significantly enhances replicative lifespan (RLS and resistance to proteotoxic stress, while reduced UPS capacity has opposing consequences. Despite tight transcriptional co-regulation of the UPS and oxidative detoxification systems, the impact of proteasome capacity on lifespan is independent of the latter, since elimination of Yap1, a key regulator of the oxidative stress response, does not affect lifespan extension of cells with higher proteasome capacity. Moreover, since elevated proteasome capacity results in improved clearance of toxic huntingtin fragments in a yeast model for neurodegenerative diseases, we speculate that the observed lifespan extension originates from prolonged elimination of damaged proteins in old mother cells. Epistasis analyses indicate that proteasome-mediated modulation of lifespan is at least partially distinct from dietary restriction, Tor1, and Sir2. These findings demonstrate that UPS capacity determines yeast RLS by a mechanism that is distinct

  19. Comparative endocrinology of aging and longevity regulation

    Directory of Open Access Journals (Sweden)

    John eAllard

    2011-11-01

    Full Text Available Hormones regulate growth, development, metabolism, and other complex processes in multicellular animals. For many years it has been suggested that hormones may also influence the rate of the aging process. Aging is a multifactorial process that causes biological systems to break down and cease to function in adult organisms as time passes, eventually leading to death. The exact underlying causes of the aging process remain a topic for debate, and clues that may shed light on these causes are eagerly sought after. In the last two decades, gene mutations that result in delayed aging and extended longevity have been discovered, and many of the affected genes have been components of endocrine signaling pathways. In this review we summarize the current knowledge on the roles of endocrine signaling in the regulation of aging and longevity in various animals. We begin by discussing the notion that conserved systems, including endocrine signaling pathways, regulate the aging process. Findings from the major model organisms: worms, flies and rodents, are then outlined. Unique lessons from studies of non-traditional models: bees, salmon and naked mole rats, are also discussed. Finally, we summarize the endocrinology of aging in humans, including changes in hormone levels with age, and the involvement of hormones in aging-related diseases. The most well studied and widely conserved endocrine pathway that affects aging is the insulin/insulin-like growth factor system. Mutations in genes of this pathway increase the lifespan of worms, flies, and mice. Population genetic evidence also suggests this pathway’s involvement in human aging. Other hormones including steroids have been linked to aging only in a subset of the models studied. Because of the value of comparative studies, it is suggested that the aging field could benefit from adoption of additional model organisms.

  20. Updated neuronal scaling rules for the brains of Glires (rodents/lagomorphs).

    Science.gov (United States)

    Herculano-Houzel, Suzana; Ribeiro, Pedro; Campos, Leandro; Valotta da Silva, Alexandre; Torres, Laila B; Catania, Kenneth C; Kaas, Jon H

    2011-01-01

    Brain size scales as different functions of its number of neurons across mammalian orders such as rodents, primates, and insectivores. In rodents, we have previously shown that, across a sample of 6 species, from mouse to capybara, the cerebral cortex, cerebellum and the remaining brain structures increase in size faster than they gain neurons, with an accompanying decrease in neuronal density in these structures [Herculano-Houzel et al.: Proc Natl Acad Sci USA 2006;103:12138-12143]. Important remaining questions are whether such neuronal scaling rules within an order apply equally to all pertaining species, and whether they extend to closely related taxa. Here, we examine whether 4 other species of Rodentia, as well as the closely related rabbit (Lagomorpha), conform to the scaling rules identified previously for rodents. We report the updated neuronal scaling rules obtained for the average values of each species in a way that is directly comparable to the scaling rules that apply to primates [Gabi et al.: Brain Behav Evol 2010;76:32-44], and examine whether the scaling relationships are affected when phylogenetic relatedness in the dataset is accounted for. We have found that the brains of the spiny rat, squirrel, prairie dog and rabbit conform to the neuronal scaling rules that apply to the previous sample of rodents. The conformity to the previous rules of the new set of species, which includes the rabbit, suggests that the cellular scaling rules we have identified apply to rodents in general, and probably to Glires as a whole (rodents/lagomorphs), with one notable exception: the naked mole-rat brain is apparently an outlier, with only about half of the neurons expected from its brain size in its cerebral cortex and cerebellum. Copyright © 2011 S. Karger AG, Basel.

  1. Updated Neuronal Scaling Rules for the Brains of Glires (Rodents/Lagomorphs)

    Science.gov (United States)

    Herculano-Houzel, Suzana; Ribeiro, Pedro; Campos, Leandro; Valotta da Silva, Alexandre; Torres, Laila B.; Catania, Kenneth C.; Kaas, Jon H.

    2011-01-01

    Brain size scales as different functions of its number of neurons across mammalian orders such as rodents, primates, and insectivores. In rodents, we have previously shown that, across a sample of 6 species, from mouse to capybara, the cerebral cortex, cerebellum and the remaining brain structures increase in size faster than they gain neurons, with an accompanying decrease in neuronal density in these structures [Herculano-Houzel et al.: Proc Natl Acad Sci USA 2006;103:12138–12143]. Important remaining questions are whether such neuronal scaling rules within an order apply equally to all pertaining species, and whether they extend to closely related taxa. Here, we examine whether 4 other species of Rodentia, as well as the closely related rabbit (Lagomorpha), conform to the scaling rules identified previously for rodents. We report the updated neuronal scaling rules obtained for the average values of each species in a way that is directly comparable to the scaling rules that apply to primates [Gabi et al.: Brain Behav Evol 2010;76:32–44], and examine whether the scaling relationships are affected when phylogenetic relatedness in the dataset is accounted for. We have found that the brains of the spiny rat, squirrel, prairie dog and rabbit conform to the neuronal scaling rules that apply to the previous sample of rodents. The conformity to the previous rules of the new set of species, which includes the rabbit, suggests that the cellular scaling rules we have identified apply to rodents in general, and probably to Glires as a whole (rodents/lagomorphs), with one notable exception: the naked mole-rat brain is apparently an outlier, with only about half of the neurons expected from its brain size in its cerebral cortex and cerebellum. PMID:21985803

  2. Animal remains of the Neolithic Ritual Burial Complex near Krum Village (Haskovo Region, SE Bulgaria

    Directory of Open Access Journals (Sweden)

    Zlatozar Boev

    2014-10-01

    Full Text Available From a total of 389 highly fragmented animal bone and tooth finds, dated ca. 6500-6300 B.P. have been identified 7 taxa: Lesser mole rat, Eurasian beaver, Cattle, Sheep, Goat, Pig, and Donkey.

  3. Light perception in two strictly subterranean rodents: life in the dark or blue?

    Directory of Open Access Journals (Sweden)

    Ondrej Kott

    Full Text Available BACKGROUND: The African mole-rats (Bathyergidae, Rodentia are strictly subterranean, congenitally microphthalmic rodents that are hardly ever exposed to environmental light. Because of the lack of an overt behavioural reaction to light, they have long been considered to be blind. However, recent anatomical studies have suggested retention of basic visual capabilities. In this study, we employed behavioural tests to find out if two mole-rat species are able to discriminate between light and dark, if they are able to discriminate colours and, finally, if the presence of light in burrows provokes plugging behaviour, which is assumed to have a primarily anti-predatory function. METHODOLOGY/PRINCIPAL FINDING: We used a binary choice test to show that the silvery mole-rat Heliophobius argenteocinereus and the giant mole-rat Fukomys mechowii exhibit a clear photoavoidance response to full-spectrum ("white", blue and green-yellow light, but no significant reaction to ultraviolet or red light during nest building. The mole-rats thus retain dark/light discrimination capabilities and a capacity to perceive short to medium-wavelength light in the photopic range of intensities. These findings further suggest that the mole-rat S opsin has its absorption maximum in the violet/blue part of the spectrum. The assay did not yield conclusive evidence regarding colour discrimination. To test the putative role of vision in bathyergid anti-predatory behaviour, we examined the reaction of mole-rats to the incidence of light in an artificial burrow system. The presence of light in the burrow effectively induced plugging of the illuminated tunnel. CONCLUSION/SIGNIFICANCE: Our findings suggest that the photopic vision is conserved and that low acuity residual vision plays an important role in predator avoidance and tunnel maintenance in the African mole-rats.

  4. Naked Nurse: My Life in Nursing MacFarlane Rose Naked Nurse: My Life in Nursing 476pp £10 Rose TintedSpecs Imprint 9780954451899 0954451899 [Formula: see text].

    Science.gov (United States)

    2014-08-28

    ROSE MACFARLANE'S memoirs are a delightful read. The book begins with Rose's early family life in Derbyshire and Cornwall, and nurse training at Sheffield's Royal Hallamshire Hospital. She writes about the ups and downs of her life and work in London in the 1960s - marriages, divorces, childbirth, and nursing.

  5. The "Naked Truth" Isn't Very Revealing: A Response to Wade Nelson on School Reform in Minnesota.

    Science.gov (United States)

    Pitton, Debra Eckerman

    1999-01-01

    A participant in Minnesota's standards-based reform initiative claims Wade Nelson, in his May 1998 "Kappan" article, is misinformed about teachers' exclusion from this movement. The standards constitute a minimal competence level in reading, math, and writing, were developed cooperatively, and have raised teacher expectations of…

  6. Naked, Deformed, Violated Body. A Montage in the Histoire(s) du cinema of Jean-Luc Godard

    National Research Council Canada - National Science Library

    Alberto Brodesco

    2013-01-01

    The article analyses Histoire(s) du cinéma (1988-1998), a cinematic essay by Jean-Luc Godard, and in particular it focuses on the controversial montage in which the French director aligns extracts from a pornographic film, Tod...

  7. Magnetic Lateral Flow Strip for the Detection of Cocaine in Urine by Naked Eyes and Smart Phone Camera.

    Science.gov (United States)

    Wu, Jing; Dong, Mingling; Zhang, Cheng; Wang, Yu; Xie, Mengxia; Chen, Yiping

    2017-06-05

    Magnetic lateral flow strip (MLFS) based on magnetic bead (MB) and smart phone camera has been developed for quantitative detection of cocaine (CC) in urine samples. CC and CC-bovine serum albumin (CC-BSA) could competitively react with MB-antibody (MB-Ab) of CC on the surface of test line of MLFS. The color of MB-Ab conjugate on the test line relates to the concentration of target in the competition immunoassay format, which can be used as a visual signal. Furthermore, the color density of the MB-Ab conjugate can be transferred into digital signal (gray value) by a smart phone, which can be used as a quantitative signal. The linear detection range for CC is 5-500 ng/mL and the relative standard deviations are under 10%. The visual limit of detection was 5 ng/mL and the whole analysis time was within 10 min. The MLFS has been successfully employed for the detection of CC in urine samples without sample pre-treatment and the result is also agreed to that of enzyme-linked immunosorbent assay (ELISA). With the popularization of smart phone cameras, the MLFS has large potential in the detection of drug residues in virtue of its stability, speediness, and low-cost.

  8. Morphotypes in Naked Amoebas (Protista: Distribution in Water Bodies of Zhytomyr and Volyn Polissia (Ukraine and Possible Ecological Significance

    Directory of Open Access Journals (Sweden)

    Patsyuk M. K.

    2014-12-01

    Full Text Available Проанализированы биотопическое и сезонное распределения видов голых амёб, принадлежащих к определённым мор- фотипам, на территории Житомирского и Волынского Полесья. Показано, что политактический и в некоторой степени ругозный и разветвлённый морфотипы имеют адаптивное значение и могли сформироваться в результате адаптации к условиям, которые характерны для олиготрофных озёр. В свою очередь, формирование ланцетообразного морфотипа может быть связано с адаптацией к низким температурам воды, а фламельного - к высоким.

  9. PLATYAMOEBA PSEUDOVANNELLIDA N. SP., A NAKED AMOEBA WITH WIDE SALT TOLERANCE ISOLATED FROM THE SALTON SEA, CALIFORNIA. (R826552)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  10. Functional characteristics of coronary vasomotor function following intramyocardial gene therapy with naked DNA encoding for vascular endothelial growth factor 165

    NARCIS (Netherlands)

    Tio, RA; Wijpkema, JS; Tan, ES; Asselbergs, FW; Hospers, GAP; Jessurun, GAJ; Zijlstra, F

    2005-01-01

    Vascular endothelial growth factor (VEGF) is a potent angiogenic factor. VEGF gene therapy improves perfusion of ischemic myocardium in experimental models and possibly in patients with end-stage coronary artery disease. In addition to its proliferative and migratory effect on endothelial cells, it

  11. 'Because we see them naked' - nurses' experiences in caring for hospitalized patients with dementia: considering artificial nutrition or hydration (ANH).

    Science.gov (United States)

    Bryon, Els; Dierckx de Casterlé, Bernadette; Gastmans, Chris

    2012-07-01

    The aim of this study was to explore and describe how Flemish nurses experience their involvement in the care of hospitalized patients with dementia, particularly in relation to artificial nutrition or hydration (ANH). We interviewed 21 hospital nurses who were carefully selected from nine hospitals in different regions of Flanders. 'Being touched by the vulnerability of the demented patient' was the central experience of the nurses, having great impact on them professionally as well as personally. This feeling can be described as encompassing the various stages of the care process: the nurses' initial meeting with the vulnerable patient; the intense decision-making process, during which the nurses experienced several intense emotions influenced by supporting or hindering contextual factors; and the final coping process, a time when nurses came to terms with this challenging experience. From our examination of this care process, it is obvious that nurses' involvement in ANH decision-making processes that concern patients with dementia is a difficult and ethically sensitive experience. On the one hand, the feeling of 'being touched' can imply strength, as it demonstrates that nurses are willing to provide good care. On the other hand, the feeling of 'being touched' can also imply weakness, as it makes nurses vulnerable to moral distress stemming from contextual influences. Therefore, nurses have to be supported as they carry out this ethically sensitive assignment. Practical implications are given. © 2011 Blackwell Publishing Ltd.

  12. A journey across the wall of varicose veins: what physicians do not often see with the naked eye.

    Science.gov (United States)

    Renno, W M; Saleh, F; Wali, M

    2006-01-01

    To examine varicose veins (VVs) from inside out in order to help surgeons and general practitioners better understand the pathogenesis of the disease and improve their management. A comprehensive examination of the wall of VVs was performed using transmission electron microscopy. The ultrastructural morphology of the collagen, elastin and smooth muscle content of the wall was analyzed in a sample of 10 patients (4 male and 6 female) and 10 matched controls aged between 37 and 50 years. Analysis of the tunica media revealed that the smooth muscle cells were significantly separated from each other by a marked increase in amorphous and fibrous tissue in which many of the collagen and elastin fibers lost their normal structural arrangement. The cells contained a large number of membrane-bound intracellular vesicles and cytoplasmic vacuoles. The collagen fibers were smaller and thinner than what is commonly seen in normal veins, and they were widely separated from each other. A light electron-lucent center was observed in the middle of the fibers. Similar changes were also seen in the intima and were associated with irregular plaque-like intimal thickening. Our study revealed a significant separation among smooth muscle cells in the wall of VVs, and the presence of an abnormal amorphous extracellular matrix and intracytoplasmic vacuoles could reflect 'unusual' possible secretory and phagocytic roles of smooth muscle cells. This could provide an important explanation for the abnormal contractile function of these cells in VVs.

  13. The naked and the dead: the ABCs of gymnosperm reproduction and the origin of the angiosperm flower.

    Science.gov (United States)

    Melzer, Rainer; Wang, Yong-Qiang; Theissen, Günter

    2010-02-01

    20 years after establishment of the ABC model many of the molecular mechanisms underlying development of the angiosperm flower are relatively well understood. Central players in the gene regulatory network controlling flower development are SQUA-like, DEF/GLO-like, AG-like and AGL6/SEP1-like MIKC-type MADS-domain transcription factors. These provide class A, class B, class C and the more recently defined class E floral homeotic functions, respectively. There is evidence that the floral homeotic proteins recognize the DNA of target genes in an organ-specific way as multimeric protein complexes, thus constituting 'floral quartets'. In contrast to the detailed insights into flower development, how the flower originated during evolution has remained enigmatic. However, while orthologues of all classes of floral homeotic genes appear to be absent from all non-seed plants, DEF/GLO-like, AG-like, and AGL6-like genes have been found in diverse extant gymnosperms, the closest relatives of the angiosperms. While SQUA-like and SEP1-like MADS-box genes appear to be absent from extant gymnosperms, reconstruction of MADS-box gene phylogeny surprisingly suggests that the most recent common ancestor of gymnosperms and angiosperms possessed representatives of both genes, but that these have been lost in the lineage that led to extant gymnosperms. Expression studies and genetic complementation experiments indicate that both angiosperm and gymnosperm AG-like and DEF/GLO-like genes have conserved functions in the specification of reproductive organs and in distinguishing male from female organs, respectively. Based on these findings novel models about the molecular basis of flower origin, involving changes in the expression patterns of DEF/GLO-like or AGL6/SEP1/SQUA-like genes in reproductive structures, were developed. While in angiosperms SEP1-like proteins play an important role in floral quartet formation, preliminary evidence suggests that gymnosperm DEF/GLO-like and AG-like proteins alone can already form floral quartet-like complexes, further corroborating the view that the formation of floral quartet-like complexes predated flower origin during evolution.

  14. The Social and Economic Effects of Deterioration in Health: ‘Naked-eye’ Evidence from a European Panel Survey

    Directory of Open Access Journals (Sweden)

    Antigone Lyberaki

    2010-11-01

    Full Text Available This paper examines the mobilization of social reserves in action in a large European sample survey. The longitudinal dataset of the Survey of Health, Ageing and Retirement in Europe (SHARE is used to identify individuals whose health underwent a major deterioration. The objective is to give a characterization of the problems faced by individuals in different parts of Europe when confronted by similar problems. Though the prevalence of health deterioration was roughly uniform across countries, men’s health impact rises sharply with age, while women appear to accept deterioration as a natural part of ageing. Health deterioration is instrumental in driving individuals out of employment, especially in the South. Treatment styles are very different, with emphasis on hospitals in central Europe, and Doctor visits more common in the South.

  15. Naked, Deformed, Violated Body. A Montage in the Histoire(s du cinema of Jean-Luc Godard

    Directory of Open Access Journals (Sweden)

    Alberto Brodesco

    2013-07-01

    Full Text Available The article analyses Histoire(s du cinéma (1988-1998, a cinematic essay by Jean-Luc Godard, and in particular it focuses on the controversial montage in which the French director aligns extracts from a pornographic film, Tod Browning’s Freaks, and footage from the concentration camps. With this sequence Godard inquires his own theory of montage: the idea of a productive reconciliation between opposing realities. This shocking sequence (the violence of images is compared to a similar shock (the violence of asking to witness produced by a scene of the documentary Shoah by Claude Lanzmann. The trauma of Godard’s editing choice induces the viewer to examine the issues of the degradation of the indexical status of the film, the limits of representation and the ethics of the gaze.

  16. Real-time Generation of Multi-view Naked-eye 3D Vision and Its Evaluation

    OpenAIRE

    追永, 侑平; OINAGA, Yuhei

    2013-01-01

    In recent years, with the development of three-dimensional imaging technique, as seen in moviesand games such as 3D, stereoscopic video content is becoming more familiar. Among them, theauto-stereoscopic technology can enjoy stereoscopic images without the use of special equipment,e.g., the 3D glasses. Auto-stereoscopic multi-view techniques have been studied by combiningimages which have the minute plurality of viewpoints into one image in focus. However,conventional techniques for generatin...

  17. Vaccination with pcDNA3-15/60 Naked DNA Encoding the Surface Proteinof Sporozoites in Cryptosporidium parvum

    Institute of Scientific and Technical Information of China (English)

    HEHong-xuan; ZHANGXi-chen; YINJi-gang; LIJian-hua; YANGJu

    2004-01-01

    The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidium parvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. The eukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene into pcDNA3 (+) in Xho Ⅰ and EcoR Ⅰ. A vaccination protocol was the adult pregnant goats inoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infected with C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce the immune response of goats and the vaccinated goats can transfer the immunity to offspring conferring protection against C.parvum infection. These suggested that the recombinant plasmid could be a DNA vaccine candidate.

  18. Vaccination with pcDNA3-15/60 Naked DNA Encoding the Surface Protein of Sporozoites in Cryptosporidium parvum

    Institute of Scientific and Technical Information of China (English)

    HE Hong-xuan; ZHANG Xi-chen; YIN Ji-gang; LI Jian-hua; YANG Ju

    2004-01-01

    The CP15/60 gene encoding the CP15/60 surface protein of sporozoites in Cryptosporidium parvum was obtained by PCR so as to research the nucleic vaccine against C.parvum. The eukaryotic expressing vector pcDNA3-15/60 was constructed by inserting CP15/60 gene into pcDNA3 (+) in Xho Ⅰ and EcoR Ⅰ. A vaccination protocol was the adult pregnant goats inoculated intranasally with the pcDNA3-15/60 plasmid and their offspring were infected with C.parvum oocysts. The results showed that the pcDNA3-15/60 plasmid can induce the immune response of goats and the vaccinated goats can transfer the immunity to offspring conferring protection against C.parvum infection. These suggested that the recombinant plasmid could be a DNA vaccine candidate.

  19. Rhodamine B immobilized on hollow Au-HMS material for naked-eye detection of Hg2+ in aqueous media.

    Science.gov (United States)

    Zhang, Na; Li, Gang; Cheng, Zhuhong; Zuo, Xiujin

    2012-08-30

    A simple, effective method has been demonstrated to immobilize Rhodamine B (RhB) probes on mesoporous silica (Au-HMS). The prepared chemosensor (Au-HMS-Probe) was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), UV-vis spectrum and Fourier transform infrared spectroscopy (FT-IR). Further application of Au-HMS-Probe in sensing Hg(2+) was confirmed by fluorescence titration experiment. Au-HMS-Probe afforded "turn-on" fluorescence enhancement and displayed high brightness in water, and it also showed excellent selectivity for Hg(2+) over alkali (Na(+), K(+)), alkaline earth (Mg(2+), Ca(2+)) and other heavy metal ions (Ag(+), Cd(2+), Co(2+), Pb(2+), Ni(2+), Cu(2+), Fe(2+)). Importantly, Au-HMS-Probe could be regenerated by treatment with tetrapropylammonium hydroxide solution.

  20. 'Naked' and hydrated conformers of the conserved core pentasaccharide of N-linked glycoproteins and its building blocks.

    Science.gov (United States)

    Barry, Conor S; Cocinero, Emilio J; Carçabal, Pierre; Gamblin, David P; Stanca-Kaposta, E Cristina; Remmert, Sarah M; Fernández-Alonso, María C; Rudić, Svemir; Simons, John P; Davis, Benjamin G

    2013-11-13

    N-glycosylation of eukaryotic proteins is widespread and vital to survival. The pentasaccharide unit -Man3GlcNAc2- lies at the protein-junction core of all oligosaccharides attached to asparagine side chains during this process. Although its absolute conservation implies an indispensable role, associated perhaps with its structure, its unbiased conformation and the potential modulating role of solvation are unknown; both have now been explored through a combination of synthesis, laser spectroscopy, and computation. The proximal -GlcNAc-GlcNAc- unit acts as a rigid rod, while the central, and unusual, -Man-β-1,4-GlcNAc- linkage is more flexible and is modulated by the distal Man-α-1,3- and Man-α-1,6- branching units. Solvation stiffens the 'rod' but leaves the distal residues flexible, through a β-Man pivot, ensuring anchored projection from the protein shell while allowing flexible interaction of the distal portion of N-glycosylation with bulk water and biomolecular assemblies.

  1. Half-Naked yet Empowered? :  Spartacus (2010-) (Ancient) Gender Equality in Contemporary Television

    OpenAIRE

    Foka, Anna

    2013-01-01

    Spartacus is attested in several ancient sources (Plutarch, Appian, and Florus) as the leader of a rebellion against the Roman Republic (73 to 71 BC). Despite that none of Spartacus' historical representations overtly suggest that he aimed at reforming Roman society by abolishing slavery and promoting equality, he has inspired a number of intellectuals across times and cultures as a egalitarian leader. Marx, in the Manchester letter to Engels (1861), considered him a real representative of th...

  2. Ground-based investigation of atomic oxygen effects on naked Ag and Ag with protective organic coatings

    Institute of Scientific and Technical Information of China (English)

    张蕾; 严川伟; 屈庆; 孙刚; 童靖宇; 曹楚南

    2002-01-01

    Protective behavior of organic coatings of alkyd, epoxy, urethane and siloxane applied to silver solar cell interconnects and erosion of Ag were investigated by exposure experiments to ground simulation atomic oxygen (AO). Evaluations of their protective properties were made through assessing their mass change characteristics, chemical and physical changes to AO attack. The specimens after exposure were analyzed with SEM/EDAX and XPS. The results indicated that the silver is heavily etched, some coatings such as epoxy, alkyd, urethane are seriously degraded or removed as volatile oxides and the morphology of coatings changed. It is evident that siloxane coatings have excellent properties for anti-AO attack. The erosion product consists of silicon dioxide layer of compact texture and is left on the surface to provide adequate protection from further attack by the energetic AO.

  3. On the nest of Cryptomys Hottentotus in the Kruger National Park.

    Directory of Open Access Journals (Sweden)

    M.Sc.(Rand, G. De Graaff

    1962-05-01

    Full Text Available This paper briefly describes the structure of a nest of the mole rat, Cryptomys hotfentotus. In the Pretoriuskop area this species uses the gifbol fBop/ione disticha, grass roots and the sekelbos (Dichrosfachys nyassana as the main material for nest construction. The presence of invertebrates in the nest (such as mites and beetles is mentioned.

  4. Search for high-energy muon neutrinos from the "naked-eye" GRB 080319B with the IceCube neutrino telescope

    DEFF Research Database (Denmark)

    Abbasi, R.; Abdou, Y.; Abu-Zayyad, T.

    2009-01-01

    We report on a search with the IceCube detector for high-energy muon neutrinos from GRB 080319B, one of the brightest gamma-ray bursts (GRBs) ever observed. The fireball model predicts that a mean of 0.1 events should be detected by IceCube for a bulk Lorentz boost of the jet of 300. In both...... the direct on-time window of 66 s and an extended window of about 300 s around the GRB, no excess was found above background. The 90% CL upper limit on the number of track-like events from the GRB is 2.7, corresponding to a muon neutrino fluence limit of 9.5x10^-3 erg cm^-2 in the energy range between 120 Te...

  5. Expression of chicken parvovirus VP2 in chicken embryo fibroblasts requires codon optimization for production of naked DNA and vectored meleagrid herpesvirus type 1 vaccines.

    Science.gov (United States)

    Spatz, Stephen J; Volkening, Jeremy D; Mullis, Robert; Li, Fenglan; Mercado, John; Zsak, Laszlo

    2013-10-01

    Meleagrid herpesvirus type 1 (MeHV-1) is an ideal vector for the expression of antigens from pathogenic avian organisms in order to generate vaccines. Chicken parvovirus (ChPV) is a widespread infectious virus that causes serious disease in chickens. It is one of the etiological agents largely suspected in causing Runting Stunting Syndrome (RSS) in chickens. Initial attempts to express the wild-type gene encoding the capsid protein VP2 of ChPV by insertion into the thymidine kinase gene of MeHV-1 were unsuccessful. However, transient expression of a codon-optimized synthetic VP2 gene cloned into the bicistronic vector pIRES2-Ds-Red2, could be demonstrated by immunocytochemical staining of transfected chicken embryo fibroblasts (CEFs). Red fluorescence could also be detected in these transfected cells since the red fluorescent protein gene is downstream from the internal ribosome entry site (IRES). Strikingly, fluorescence could not be demonstrated in cells transiently transfected with the bicistronic vector containing the wild-type or non-codon-optimized VP2 gene. Immunocytochemical staining of these cells also failed to demonstrate expression of wild-type VP2, indicating that the lack of expression was at the RNA level and the VP2 protein was not toxic to CEFs. Chickens vaccinated with a DNA vaccine consisting of the bicistronic vector containing the codon-optimized VP2 elicited a humoral immune response as measured by a VP2-specific ELISA. This VP2 codon-optimized bicistronic cassette was rescued into the MeHV-1 genome generating a vectored vaccine against ChPV disease.

  6. 莜麦在河南省引种试验的研究%Studies on introduction trial of naked oat(Avena nuda) in Henan province

    Institute of Scientific and Technical Information of China (English)

    郭孝; 哈斯

    2004-01-01

    通过对莜麦Avena nuda的形态特征、生物学特征、产量以及营养价值的分析,对其利用价值进行了评价.研究结果表明:莜麦能够很好地适应河南省的土壤及气候条件,生长快,叶量丰富,产量高,在河南省有良好的推广价值,可望成为当地重要的饲料作物和粮食作物.

  7. "You're naked, you're vulnerable": Sexual well-being and body image of women with lower limb lymphedema.

    Science.gov (United States)

    Winch, Caleb J; Sherman, Kerry A; Smith, Katriona M; Koelmeyer, Louise A; Mackie, Helen; Boyages, John

    2016-09-01

    Lower-limb lymphedema is an incurable illness manifesting as visible swelling enlarging the leg(s) and/or feet, buttocks, and genitals. This study used semi-structured interviews and thematic analysis to explore sexual well-being among women with primary (congenital) lymphedema (n=11) or secondary lymphedema associated with gynecological cancer (n=8). Five themes (subthemes) summarized women's responses, with Attractiveness and Confidence (Publicly Unattractive, Privately Unconfident, Lymphedema or Aging?) describing women's central concern. These body image-related concerns accounted for sexual well-being in association with Partner Support (Availability of Support, Languages of Support, Fears About Support) and the degree of Functional Interruptions (Lymphedema in Context, Enduring Impacts, Overcoming Interruptions). Successful Lymphedema Coping (Control, Acceptance) and self-perceived ability to fulfill a valued Sexual Role also affected sexual well-being. Few differences between women with primary versus secondary lymphedema were evident. Lymphedema clinicians should screen for sexual concerns and have referral options available. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. When the number crunchers took on the particle smashers in a race to expose the naked quark, the stakes could hardly have been higher

    CERN Multimedia

    MacKenzie, Dana

    2005-01-01

    It was a true clash of the titans. In the blue corner: a multimilliion-dollar particle accelerator. In the red: one of the world's most powerful supecomputers. Both were battling to pin down the lifetime of an ephemeral subatomic particle known as the D-meson (3 pages)

  9. Celestial polarization patterns sufficient for Viking navigation with the naked eye: detectability of Haidinger's brushes on the sky versus meteorological conditions.

    Science.gov (United States)

    Horváth, Gábor; Takács, Péter; Kretzer, Balázs; Szilasi, Szilvia; Száz, Dénes; Farkas, Alexandra; Barta, András

    2017-02-01

    If a human looks at the clear blue sky from which light with high enough degree of polarization d originates, an 8-shaped bowtie-like figure, the yellow Haidinger's brush can be perceived, the long axis of which points towards the sun. A band of high d arcs across the sky at 90° from the sun. A person can pick two points on that band, observe the yellow brushes and triangulate the position of the sun based on the orientation of the two observed brushes. This method has been suggested to have been used on the open sea by Viking navigators to determine the position of the invisible sun occluded by cloud or fog. Furthermore, Haidinger's brushes can also be used to locate the sun when it is below the horizon or occluded by objects on the horizon. To determine the position of the sun using the celestial polarization pattern, the d of the portion of the sky used must be greater than the viewer's degree of polarization threshold d* for perception of Haidinger's brushes. We studied under which sky conditions the prerequisite d > d* is satisfied. Using full-sky imaging polarimetry, we measured the d-pattern of skylight in the blue (450 nm) spectral range for 1296 different meteorological conditions with different solar elevation angles θ and per cent cloud cover ρ. From the measured d-patterns of a given sky we determined the proportion P of the sky for which d > d*. We obtained that P is the largest at low solar elevations θ ≈ 0° and under totally or nearly clear skies with cloud coverage ρ = 0%, when the sun's position is already easily determined. If the sun is below the horizon (-5° ≤ θ see text] under clear sky conditions. Consequently, the sky-polarimetric Viking navigation based on Haidinger's brushes is most useful after sunset and prior to sunrise, when the sun is not visible and large sky regions are bright, clear and polarized enough for perception of Haidinger's brushes.

  10. Analysis about Naked Wedding of 80' generation in China%“80后”裸婚的成因分析

    Institute of Scientific and Technical Information of China (English)

    石燕

    2012-01-01

    通过对“80后”进行深入的实地调查,以五位1980年后出生的被访者为个案研究对象,以开放式访谈收集资料,在对资料进行质性分析的基础上发现:这批“80后”并非自愿选择裸婚,而是由于婚姻成本的畸高、婚姻中感情因素的上升、婚姻双方对婚后家庭生活的重视以及代际间财富流的变化等原因造成的。%By making a survey of five interviewers whoever all born after 1980, qualitative research on the information gathered by open-minded conversations with them indicates that they are not willing to choose nude wedding but have to do it for reasons. Extra high cost of marriage, high expectation for family life after marriage and change in flow of wealth between inter-generation are the main reasons.

  11. Electroanalytical and naked eye determination of Cu(2+) ion in various environmental samples using 5-amino-1,3,4-thiadiazole-2-thiol based Schiff bases.

    Science.gov (United States)

    Bandi, Koteswara Rao; Singh, Ashok Kumar; Upadhyay, Anjali

    2014-01-01

    Novel polydentate Schiff bases 4-(5-mercapto-1,3,4-thiadiazol-2-ylimino)pentan-2-one (S1) and (2-(indol-3-yl)vinyl)-1,3,4-thiadiazole-2-thiol (S2) were synthesized and explored as Cu(2+) selective polymeric membrane electrodes (PME) using different plasticizers and anionic excluders. The potentiometric data revealed that the PME having the membrane composition (S2: NaTPB: TBP: PVC as 4: 2: 58: 36 (w/w; mg)) is shown to have good results. Thus the coated graphite electrode (CGE) with the same composition as the best PME was also fabricated and investigated as Cu(2+) selective electrode. It was found that CGE showed better response characteristics than PME with respect to low detection limit (1.2×10(-8)molL(-1)), near Nernstian slope (29.8±0.4mV decade(-1) of activity), wide working concentration range (6.4×10(-8)-1.0×10(-1)molL(-1)), long shelf life (90days) and fast response time (9s). The CGE was used successfully as an indicator electrode for the potentiometric determination of Cu(2+) ion against EDTA and also used to quantify Cu(2+) ion in soil, water, medicinal plants, vegetables and edible oil samples. The Schiff base S2 is used as chemosensor for the selective determination of Cu(2+) ion.

  12. Are all seeds of gymnosperms naked%裸子植物的种子是否都是裸露的

    Institute of Scientific and Technical Information of China (English)

    李新华

    2005-01-01

    裸子植物的胚珠虽然在传粉时是裸露的,但在发育成种子的过程中,罗汉松科、三尖杉科、红豆杉科、买麻藤科、麻黄科及柏科的圆柏属(Sabina)和刺柏属(Juniperus)等类群的种子在成熟时常常为肉质的假种皮或种鳞等组织所包被.因此,并不是所有裸子植物类群的种子在成熟时都是裸露的.裸子植物种子与其周围肉质假种皮或种鳞等组织共同构成的繁殖结构,尽管在结构上与被子植物的果实具有根本的区别,但在吸引鸟类等食果动物传播种子的生态功能方面却与被子植物的肉质果实是相同的.

  13. In-situ one-step synthesis of carbon-encapsulated naked magnetic metal nanoparticles conducted without additional reductants and agents

    Science.gov (United States)

    Kang, Jun; Kim, Yeonwon; Kim, Hye-Min; Hu, Xiulan; Saito, Nagahiro; Choi, Jae-Hyuk; Lee, Myeong-Hoon

    2016-12-01

    C-encapsulated highly pure Ni, Co, and Fe magnetic nanoparticles (MNPs/C) were synthesized by an innovative one-step in-situ plasma in liquid method (solution plasma processing, SPP) without any additional reductants, agents, or treatment. Successful encapsulation of MNPs was demonstrated by using inductively coupled plasma-atomic emission spectrometry and cyclic voltammetry techniques. The obtained X-ray diffraction patterns and transmission electron microscopy images corresponded to MNPs with average diameters of 5 nm and good crystalline structure. The C capsules with spherical shapes (containing onion-like layers) were characterized by uniform sizes (ranging from 20 nm to 30 nm) and chain-like morphologies. The synthesized MNPs/C exhibited superparamagnetic properties at room temperature and might be utilized in data storage, biomedical, and energy applications since various NPs (including bimetallic ones) could be easily prepared by changing working electrodes. This study shows the potential of SPP to be a candidate for the next-generation synthesis method of NPs/C.

  14. (Almost) naked quantum criticality with non-Fermi liquid behavior at the onset of inhomogeneous Larkin-Ovchinikov superfluidity in two dimensions

    Science.gov (United States)

    Strack, Philipp; Piazza, Francesco

    2015-03-01

    We present a renormalization group analysis for the non-Fermi liquid behavior and quantum criticality arising in coupled quantum wires of attractively interacting fermions with spin imbalance in two spatial dimensions.

  15. Ab initio structures for 90 degrees -twisted s-trans-1,3-butadiene and cyclooctatetraene: the naked sp2-sp2 bond.

    Science.gov (United States)

    Feller, David; Craig, Norman C; Matlin, Albert R

    2008-03-20

    The bond length of a carbon-carbon sp2-sp2 sigma-bond without the perturbing effects of pi-interactions has been estimated by high level calculations on two prototypical systems: a 90 degrees -twisted form of butadiene and the tub conformer of cyclooctatetraene. The former system yields a value of 1.4818 A, considerably longer than previous estimates. The corresponding bond length in the latter is slightly shorter due to some pi-electron delocalization.

  16. 莜麦馒头微波蒸制工艺的研究%Preparation of Naked Oat Steamed Bread by Microwave

    Institute of Scientific and Technical Information of China (English)

    汪磊; 云月英; 王国泽; 李俊芳; 莎娜; 张欢子

    2014-01-01

    采用响应面法研究微波功率、微波时间和微波装载量对莜麦馒头品质的影响,确定莜麦馒头微波蒸制的最佳工艺条件,并比较了微波蒸制和常规蒸汽蒸制2个烹制方式对莜麦馒头的影响.结果表明,微波蒸制莜麦馒头的最佳工艺为:微波功率560 W,微波时间12.5 min,装载量74 g.按该工艺条件制作的莜麦馒头与蒸汽蒸制莜麦馒头的感官评分没有显著性差异,与蒸汽蒸制莜麦馒头相比,微波莜麦馒头水分含量较低,硬度和黏着性大,弹性差,而回复性和咀嚼性优于蒸汽蒸制莜麦馒头.

  17. Novel synthetic (S,S) and (R,R)-secoisolariciresinol diglucosides (SDGs) protect naked plasmid and genomic DNA From gamma radiation damage.

    Science.gov (United States)

    Mishra, Om P; Pietrofesa, Ralph; Christofidou-Solomidou, Melpo

    2014-07-01

    Secoisolariciresinol diglucoside (SDG) is the major lignan in wholegrain flaxseed. However, extraction methods are complex and are associated with low yield and high costs. Using a novel synthetic pathway, our group succeeded in chemically synthesizing SDG (S,S and R,R enantiomers), which faithfully recapitulates the properties of their natural counterparts, possessing strong antioxidant and free radical scavenging properties. This study further extends initial findings by now investigating the DNA-radioprotective properties of the synthetic SDG enantiomers compared to the commercial SDG. DNA radioprotection was assessed by cell-free systems such as: (a) plasmid relaxation assay to determine the extent of the supercoiled (SC) converted to open-circular (OC) plasmid DNA (pBR322) after exposure of the plasmid to gamma radiation; and (b) determining the extent of genomic DNA fragmentation. Exposure of plasmid DNA to 25 Gy of γ radiation resulted in decreased supercoiled form and increased open-circular form, indicating radiation-induced DNA damage. Synthetic SDG (S,S) and SDG (R,R), and commercial SDG at concentrations of 25-250 μM significantly and equipotently reduced the radiation-induced supercoiled to open-circular plasmid DNA in a dose-dependent conversion. In addition, exposure of calf thymus DNA to 50 Gy of gamma radiation resulted in DNA fragments of low-molecular weight (DNA-radioprotective properties. Such properties along with their antioxidant and free radical scavenging activity, reported earlier, suggest that SDGs are promising candidates for radioprotection for normal tissue damage as a result of accidental exposure during radiation therapy for cancer treatment.

  18. Celestial polarization patterns sufficient for Viking navigation with the naked eye: detectability of Haidinger's brushes on the sky versus meteorological conditions

    Science.gov (United States)

    Horváth, Gábor; Takács, Péter; Kretzer, Balázs; Szilasi, Szilvia; Száz, Dénes; Farkas, Alexandra; Barta, András

    2017-02-01

    If a human looks at the clear blue sky from which light with high enough degree of polarization d originates, an 8-shaped bowtie-like figure, the yellow Haidinger's brush can be perceived, the long axis of which points towards the sun. A band of high d arcs across the sky at 90° from the sun. A person can pick two points on that band, observe the yellow brushes and triangulate the position of the sun based on the orientation of the two observed brushes. This method has been suggested to have been used on the open sea by Viking navigators to determine the position of the invisible sun occluded by cloud or fog. Furthermore, Haidinger's brushes can also be used to locate the sun when it is below the horizon or occluded by objects on the horizon. To determine the position of the sun using the celestial polarization pattern, the d of the portion of the sky used must be greater than the viewer's degree of polarization threshold d* for perception of Haidinger's brushes. We studied under which sky conditions the prerequisite d > d* is satisfied. Using full-sky imaging polarimetry, we measured the d-pattern of skylight in the blue (450 nm) spectral range for 1296 different meteorological conditions with different solar elevation angles θ and per cent cloud cover ρ. From the measured d-patterns of a given sky we determined the proportion P of the sky for which d > d*. We obtained that P is the largest at low solar elevations θ ≈ 0° and under totally or nearly clear skies with cloud coverage ρ = 0%, when the sun's position is already easily determined. If the sun is below the horizon (-5° ≤ θ regions are bright, clear and polarized enough for perception of Haidinger's brushes.

  19. 联创广告,让地产告别"品牌裸奔"%Unad Let The Real Estate Abandon Naked Brand

    Institute of Scientific and Technical Information of China (English)

    张翰闻

    2010-01-01

    @@ 拳王阿里曾与小提琴大师梅纽因对话:"我们都是操手艺营生的,我遍体鳞伤,你却毫发无损!"-一个是紧张应对,有时还被动挨打;另一个是主动操控:其必然导致境界的不同.

  20. A naked-eye and ratiometric near-infrared probe for palladium via modulation of a π-conjugated system of cyanines.

    Science.gov (United States)

    Wang, Xiaohang; Guo, Zhiqian; Zhu, Shiqin; Tian, He; Zhu, Weihong

    2014-11-14

    A ratiometric and colorimetric cyanine-based palladium sensor with an excellent selectivity and sensitivity has been designed. Notably, the modulation of π-conjugated electrons in cyanine dyes can result in a ratiometric fluorescence change with a large Stokes shift (270 nm), especially for realizing palladium detection in aqueous samples using indicator paper and in living cells by ratiometric mode. The limit of detection is as low as 0.3 ppb.

  1. [Ni(cod)2][Al(ORF)4], a Source for Naked Nickel(I) Chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Schwab, Miriam M. [Univ. of Freiburg (Germany). Institut fuer Anorganische und Analytische Chemie, Freiburger Materialforschungszentrum (FMF); Himmel, Daniel [Univ. of Freiburg (Germany). Institut fuer Anorganische und Analytische Chemie, Freiburger Materialforschungszentrum (FMF); Kacprzak, Sylwia [Univ. of Freiburg (Germany). Institut fuer Physikalische Chemie; Kratzert, Daniel [Univ. of Freiburg (Germany). Institut fuer Anorganische und Analytische Chemie, Freiburger Materialforschungszentrum (FMF); Radtke, Valentin [Univ. of Freiburg (Germany). Institut fuer Anorganische und Analytische Chemie, Freiburger Materialforschungszentrum (FMF); Weis, Philippe [Univ. of Freiburg (Germany). Institut fuer Anorganische und Analytische Chemie, Freiburger Materialforschungszentrum (FMF); Ray, Kallol [Humboldt Univ. of Berlin (Germany). Inst. fuer Chemie; Scheidt, Ernst-Wilhelm [Univ. of Ausburg (Germany). Inst. fuer Physik; Scherer, Wolfgang [Univ. of Ausburg (Germany). Inst. fuer Physik; de Bruin, Bas [Univ. of Amsterdam (Netherlands). Van‘t Hoff Inst. for Molecular Sciences (HIMS); Weber, Stefan [Univ. of Freiburg (Germany). Institut fuer Physikalische Chemie; Krossing, Ingo [Univ. of Freiburg (Germany). Institut fuer Anorganische und Analytische Chemie, Freiburger Materialforschungszentrum (FMF)

    2015-10-13

    The straightforward synthesis of the cationic, purely organometallic NiI salt [Ni(cod)2]+[Al(ORF)4]- was realized through a reaction between [Ni(cod)2] and Ag[Al(ORF)4] (cod=1,5-cyclooctadiene). Crystal-structure analysis and EPR, XANES, and cyclic voltammetry studies confirmed the presence of a homoleptic NiI olefin complex. Weak interactions between the metal center, the ligands, and the anion provide a good starting material for further cationic NiI complexes.

  2. ERRATUM: "Search for High-Energy Muon Neutrinos from the "Naked-Eye" GRB 080319B with the Icecube Neutrino Telescope" (2009, ApJ, 701, 1721)

    Science.gov (United States)

    Abbasi, R.; Abdou, Y.; Ackermann, M.; Adams, J.; Ahlers, M.; Andeen, K.; Auffenberg, J.; Bai, X.; Baker, M.; Barwick, S. W.; Bay, R.; Bazo Alba, J. L.; Beattie, K.; Bechet, S.; Becker, J. K.; Becker, K.-H.; Benabderrahmane, M. L.; Berdermann, J.; Berghaus, P.; Berley, D.; Bernardini, E.; Bertrand, D.; Besson, D. Z.; Bissok, M.; Blaufuss, E.; Boersma, D. J.; Bohm, C.; Bolmont, J.; Böser, S.; Botner, O.; Bradley, L.; Braun, J.; Breder, D.; Burgess, T.; Castermans, T.; Chirkin, D.; Christy, B.; Clem, J.; Cohen, S.; Cowen, D. F.; D'Agostino, M. V.; Danninger, M.; Day, C. T.; De Clercq, C.; Demirörs, L.; Depaepe, O.; Descamps, F.; Desiati, P.; de Vries-Uiterweerd, G.; DeYoung, T.; Diaz-Velez, J. C.; Dreyer, J.; Dumm, J. P.; Duvoort, M. R.; Edwards, W. R.; Ehrlich, R.; Eisch, J.; Ellsworth, R. W.; Engdegård, O.; Euler, S.; Evenson, P. A.; Fadiran, O.; Fazely, A. R.; Feusels, T.; Filimonov, K.; Finley, C.; Foerster, M. M.; Fox, B. D.; Franckowiak, A.; Franke, R.; Gaisser, T. K.; Gallagher, J.; Ganugapati, R.; Gerhardt, L.; Gladstone, L.; Goldschmidt, A.; Goodman, J. A.; Gozzini, R.; Grant, D.; Griesel, T.; Groß, A.; Grullon, S.; Gunasingha, R. M.; Gurtner, M.; Ha, C.; Hallgren, A.; Halzen, F.; Han, K.; Hanson, K.; Hasegawa, Y.; Heise, J.; Helbing, K.; Herquet, P.; Hickford, S.; Hill, G. C.; Hoffman, K. D.; Hoshina, K.; Hubert, D.; Huelsnitz, W.; Hülß, J.-P.; Hulth, P. O.; Hultqvist, K.; Hussain, S.; Imlay, R. L.; Inaba, M.; Ishihara, A.; Jacobsen, J.; Japaridze, G. S.; Johansson, H.; Joseph, J. M.; Kampert, K.-H.; Kappes, A.; Karg, T.; Karle, A.; Kelley, J. L.; Kenny, P.; Kiryluk, J.; Kislat, F.; Klein, S. R.; Klepser, S.; Knops, S.; Kohnen, G.; Kolanoski, H.; Köpke, L.; Kowalski, M.; Kowarik, T.; Krasberg, M.; Kuehn, K.; Kuwabara, T.; Labare, M.; Laihem, K.; Landsman, H.; Lauer, R.; Leich, H.; Lennarz, D.; Lucke, A.; Lundberg, J.; Lünemann, J.; Madsen, J.; Majumdar, P.; Maruyama, R.; Mase, K.; Matis, H. S.; McParland, C. P.; Meagher, K.; Merck, M.; Mészáros, P.; Middell, E.; Milke, N.; Miyamoto, H.; Mohr, A.; Montaruli, T.; Morse, R.; Movit, S. M.; Münich, K.; Nahnhauer, R.; Nam, J. W.; Nießen, P.; Nygren, D. R.; Odrowski, S.; Olivas, A.; Olivo, M.; Ono, M.; Panknin, S.; Patton, S.; Pérez de los Heros, C.; Petrovic, J.; Piegsa, A.; Pieloth, D.; Pohl, A. C.; Porrata, R.; Potthoff, N.; Price, P. B.; Prikockis, M.; Przybylski, G. T.; Rawlins, K.; Redl, P.; Resconi, E.; Rhode, W.; Ribordy, M.; Rizzo, A.; Rodrigues, J. P.; Roth, P.; Rothmaier, F.; Rott, C.; Roucelle, C.; Rutledge, D.; Ryckbosch, D.; Sander, H.-G.; Sarkar, S.; Satalecka, K.; Schlenstedt, S.; Schmidt, T.; Schneider, D.; Schukraft, A.; Schulz, O.; Schunck, M.; Seckel, D.; Semburg, B.; Seo, S. H.; Sestayo, Y.; Seunarine, S.; Silvestri, A.; Slipak, A.; Spiczak, G. M.; Spiering, C.; Stamatikos, M.; Stanev, T.; Stephens, G.; Stezelberger, T.; Stokstad, R. G.; Stoufer, M. C.; Stoyanov, S.; Strahler, E. A.; Straszheim, T.; Sulanke, K.-H.; Sullivan, G. W.; Swillens, Q.; Taboada, I.; Tarasova, O.; Tepe, A.; Ter-Antonyan, S.; Terranova, C.; Tilav, S.; Tluczykont, M.; Toale, P. A.; Tosi, D.; Turčan, D.; van Eijndhoven, N.; Vandenbroucke, J.; Van Overloop, A.; Voigt, B.; Walck, C.; Waldenmaier, T.; Walter, M.; Wendt, C.; Westerhoff, S.; Whitehorn, N.; Wiebusch, C. H.; Wiedemann, A.; Wikström, G.; Williams, D. R.; Wischnewski, R.; Wissing, H.; Woschnagg, K.; Xu, X. W.; Yodh, G.; Yoshida, S.; IceCube Collaboration

    2010-01-01

    We have noticed some mistakes in formulae (A2) and (A5) in the appendix of our paper. The errors are not present in the code used in the analysis and hence none of the plots or results is affected. The correct formulae are

  3. Search for High-Energy Muon Neutrinos from the "Naked-Eye" GRB 080319B with the IceCube Neutrino Telescope

    Science.gov (United States)

    Abbasi, R.; Abdou, Y.; Abu-Zayyad, T.; Adams, J.; Aguilar, J. A.; Ahlers, M.; Andeen, K.; Auffenberg, J.; Bai, X.; Baker, M.; Barwick, S. W.; Bay, R.; Bazo Alba, J. L.; Beattie, K.; Beatty, J. J.; Bechet, S.; Becker, J. K.; Becker, K.-H.; Benabderrahmane, M. L.; Berdermann, J.; Berghaus, P.; Berley, D.; Bernardini, E.; Bertrand, D.; Besson, D. Z.; Bissok, M.; Blaufuss, E.; Boersma, D. J.; Bohm, C.; Bolmont, J.; Botner, O.; Bradley, L.; Braun, J.; Breder, D.; Castermans, T.; Chirkin, D.; Christy, B.; Clem, J.; Cohen, S.; Cowen, D. F.; D'Agostino, M. V.; Danninger, M.; Day, C. T.; De Clercq, C.; Demirörs, L.; Depaepe, O.; Descamps, F.; Desiati, P.; de Vries-Uiterweerd, G.; DeYoung, T.; Diaz-Velez, J. C.; Dreyer, J.; Dumm, J. P.; Duvoort, M. R.; Edwards, W. R.; Ehrlich, R.; Eisch, J.; Ellsworth, R. W.; Engdegård, O.; Euler, S.; Evenson, P. A.; Fadiran, O.; Fazely, A. R.; Feusels, T.; Filimonov, K.; Finley, C.; Foerster, M. M.; Fox, B. D.; Franckowiak, A.; Franke, R.; Gaisser, T. K.; Gallagher, J.; Ganugapati, R.; Gerhardt, L.; Gladstone, L.; Goldschmidt, A.; Goodman, J. A.; Gozzini, R.; Grant, D.; Griesel, T.; Groß, A.; Grullon, S.; Gunasingha, R. M.; Gurtner, M.; Ha, C.; Hallgren, A.; Halzen, F.; Han, K.; Hanson, K.; Hasegawa, Y.; Heise, J.; Helbing, K.; Herquet, P.; Hickford, S.; Hill, G. C.; Hoffman, K. D.; Hoshina, K.; Hubert, D.; Huelsnitz, W.; Hülß, J.-P.; Hulth, P. O.; Hultqvist, K.; Hussain, S.; Imlay, R. L.; Inaba, M.; Ishihara, A.; Jacobsen, J.; Japaridze, G. S.; Johansson, H.; Joseph, J. M.; Kampert, K.-H.; Kappes, A.; Karg, T.; Karle, A.; Kelley, J. L.; Kenny, P.; Kiryluk, J.; Kislat, F.; Klein, S. R.; Knops, S.; Kohnen, G.; Kolanoski, H.; Köpke, L.; Kowalski, M.; Kowarik, T.; Krasberg, M.; Kuehn, K.; Kuwabara, T.; Labare, M.; Lafebre, S.; Laihem, K.; Landsman, H.; Lauer, R.; Lennarz, D.; Lucke, A.; Lundberg, J.; Lünemann, J.; Madsen, J.; Majumdar, P.; Maruyama, R.; Mase, K.; Matis, H. S.; McParland, C. P.; Meagher, K.; Merck, M.; Mészáros, P.; Middell, E.; Milke, N.; Miyamoto, H.; Mohr, A.; Montaruli, T.; Morse, R.; Movit, S. M.; Münich, K.; Nahnhauer, R.; Nam, J. W.; Nießen, P.; Nygren, D. R.; Odrowski, S.; Olivas, A.; Olivo, M.; Ono, M.; Panknin, S.; Patton, S.; Pérez de los Heros, C.; Petrovic, J.; Piegsa, A.; Pieloth, D.; Pohl, A. C.; Porrata, R.; Potthoff, N.; Price, P. B.; Prikockis, M.; Przybylski, G. T.; Rawlins, K.; Redl, P.; Resconi, E.; Rhode, W.; Ribordy, M.; Rizzo, A.; Rodrigues, J. P.; Roth, P.; Rothmaier, F.; Rott, C.; Roucelle, C.; Rutledge, D.; Ryckbosch, D.; Sander, H.-G.; Sarkar, S.; Schlenstedt, S.; Schmidt, T.; Schneider, D.; Schukraft, A.; Schulz, O.; Schunck, M.; Seckel, D.; Semburg, B.; Seo, S. H.; Sestayo, Y.; Seunarine, S.; Silvestri, A.; Slipak, A.; Spiczak, G. M.; Spiering, C.; Stamatikos, M.; Stanev, T.; Stephens, G.; Stezelberger, T.; Stokstad, R. G.; Stoufer, M. C.; Stoyanov, S.; Strahler, E. A.; Straszheim, T.; Sulanke, K.-H.; Sullivan, G. W.; Swillens, Q.; Taboada, I.; Tamburro, A.; Tarasova, O.; Tepe, A.; Ter-Antonyan, S.; Terranova, C.; Tilav, S.; Toale, P. A.; Tosi, D.; Turčan, D.; van Eijndhoven, N.; Vandenbroucke, J.; Van Overloop, A.; Voigt, B.; Walck, C.; Waldenmaier, T.; Walter, M.; Wendt, C.; Westerhoff, S.; Whitehorn, N.; Wiebusch, C. H.; Wiedemann, A.; Wikström, G.; Williams, D. R.; Wischnewski, R.; Wissing, H.; Woschnagg, K.; Xu, X. W.; Yodh, G.; Yoshida, S.; IceCube Collaboration

    2009-08-01

    We report on a search with the IceCube detector for high-energy muon neutrinos from GRB 080319B, one of the brightest gamma-ray bursts (GRBs) ever observed. The fireball model predicts that a mean of 0.1 events should be detected by IceCube for a bulk Lorentz boost of the jet of 300. In both the direct on-time window of 66 s and an extended window of about 300 s around the GRB, no excess was found above background. The 90% CL upper limit on the number of track-like events from the GRB is 2.7, corresponding to a muon neutrino fluence limit of 9.5 × 10-3 erg cm-2 in the energy range between 120 TeV and 2.2 PeV, which contains 90% of the expected events.

  4. “Girls are addicted to likes so they post semi-naked selfies”: peer mediation, normativity and the construction of identity online

    OpenAIRE

    Mascheroni, Giovanna; Vincent, Jane; Jimenez, Estefanía

    2015-01-01

    This paper examines how children aged 11-16 in three European countries (Italy, UK and Spain) develop and present their online identities, and their interactions with peers. It focuses on young people’s engagement with the construction of an online identity on social media through pictures, and explores how peer-mediated conventions of self-presentation are appropriated, legitimated, or resisted in pre-teens’ and teenagers’ discourses. In doing so, we draw on Goffman’s (1959) work on the pres...

  5. Celestial polarization patterns sufficient for Viking navigation with the naked eye: detectability of Haidinger's brushes on the sky versus meteorological conditions

    Science.gov (United States)

    Takács, Péter; Kretzer, Balázs; Szilasi, Szilvia; Száz, Dénes; Farkas, Alexandra; Barta, András

    2017-01-01

    If a human looks at the clear blue sky from which light with high enough degree of polarization d originates, an 8-shaped bowtie-like figure, the yellow Haidinger's brush can be perceived, the long axis of which points towards the sun. A band of high d arcs across the sky at 90° from the sun. A person can pick two points on that band, observe the yellow brushes and triangulate the position of the sun based on the orientation of the two observed brushes. This method has been suggested to have been used on the open sea by Viking navigators to determine the position of the invisible sun occluded by cloud or fog. Furthermore, Haidinger's brushes can also be used to locate the sun when it is below the horizon or occluded by objects on the horizon. To determine the position of the sun using the celestial polarization pattern, the d of the portion of the sky used must be greater than the viewer's degree of polarization threshold d* for perception of Haidinger's brushes. We studied under which sky conditions the prerequisite d > d* is satisfied. Using full-sky imaging polarimetry, we measured the d-pattern of skylight in the blue (450 nm) spectral range for 1296 different meteorological conditions with different solar elevation angles θ and per cent cloud cover ρ. From the measured d-patterns of a given sky we determined the proportion P of the sky for which d > d*. We obtained that P is the largest at low solar elevations θ ≈ 0° and under totally or nearly clear skies with cloud coverage ρ = 0%, when the sun's position is already easily determined. If the sun is below the horizon (−5° ≤ θ < 0°) during twilight, P = 76.17 ± 4.18% for dmin∗=23% under clear sky conditions. Consequently, the sky-polarimetric Viking navigation based on Haidinger's brushes is most useful after sunset and prior to sunrise, when the sun is not visible and large sky regions are bright, clear and polarized enough for perception of Haidinger's brushes. PMID:28386426

  6. A comparison of the breeding and feeding ecology of pigeon guillemots at Naked and Jackpot Islands in Prince William Sound, Alaska

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The field work for Restoration Project 95163F was conducted during the summer of 1995. A similar effort was made in 1994 as Project 94173. Previous related projects...

  7. Naked-eye sensitive detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) based on a horseradish peroxidase catalytic colorimetric system with Cu(ii).

    Science.gov (United States)

    Shi, Dongmin; Sun, Yue; Lin, Lin; Shi, Chunjun; Wang, Guangfeng; Zhang, Xiaojun

    2016-10-07

    In this paper, a novel colorimetric method for the detection of alkaline phosphatase (ALP) and pyrophosphate (PPi) was designed based on a Cu(2+)-horseradish peroxidase (HRP)-3,3',5,5'-tetra-methylbenzidine (TMB)-H2O2 system. In the presence of ALP, l-ascorbic acid-2-phosphate (AAP) could be hydrolyzed to ascorbic acid which could reduce Cu(2+) to Cu(+) to inhibit the enzymatic activity of HRP in the colorimetric system. The change in absorbance was found to be proportional to the ALP concentration with a linear detection range and a limit of detection of 5.4 mU mL(-1). In the presence of PPi, because Cu(2+) was chelated by PPi, the conversion of Cu(ii) by AA was effectively inhibited. The color of the HRP-TMB-H2O2 system with Cu(2+) showed blue. The HRP-TMB-H2O2 system with the Cu(2+) colorimetric system could also detect PPi with a satisfying result. In summary, this method possesses sensitivity, reproducibility, and cost-effectiveness without labelling and separation and the use of a colorimetric method is more in line with the requirements of on-site detection and green chemistry.

  8. Effect of naked eukaryotic expression plasmid encoding rat augmenter of liver regeneration on acute hepatic injury and hepatic failure in rats

    Institute of Scientific and Technical Information of China (English)

    Li-Mei Zhang; Dian-Wu Liu; Jian-Bo Liu; Xiao-Lin Zhang; Xiao-Bo Wang; Long-Mei Tang; Li-Qin Wang

    2005-01-01

    AIM: To study the protective effect of eukaryotic expression plasmid encoding augmenter of liver regeneration (ALR) on acute hepatic injury and hepatic failure in rats. METHODS: The PCR-amplified ALR gene was recombined with pcDNA3 plasmid, and used to treat rats with acute hepatic injury. The rats with acute hepatic injury induced by intraperitoneal injection of 2 mL/kg 50% carbon tetrachloride (CCl4) were randomly divided into saline control group and recombinant pcDNA3-ALR plasmid treatment groups. Recombinant pcDNA3-ALR plasmid DNA (50 or 200 μg/kg) was injected into the rats with acute hepatic injury intravenously, intraperitoneally, or intravenously and intraperitoneally in combination 4 h after CCl4 administration, respectively. The recombinant plasmid was injected once per 12 h into all treatment groups four times, and the rats were decapitated 12 h after the last injection. Hepatic histopathological alterations were observed after HE staining, the expression of proliferating cell nuclear antigen (PCNA) in liver tissue was detected by immunohistochemical staining, and the level of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was determined by biochemical method. The recombinant plasmid DNA (200 μg/kg) and saline were intraperitoneally injected into the rats with acute hepatic failure induced by intraperitoneal injection of 4 mL/kg 50% CCl4 after 4 h of CCl4 administration, respectively. Rats living over 96 h were considered as survivals.RESULTS: The sequence of ALR cDNA of recombinant pcDNA3-ALR plasmid was accordant with the reported sequence of rat ALR cDNA. After the rats with acute hepatic injury were treated with recombinant pcDNA3-ALR plasmid, the degree of liver histopathological injury markedly decreased. The pathologic liver tissues, in which hepatic degeneration and necrosis of a small amount of hepatocytes and a large amount of infiltrating inflammatory cells were observed, and they became basically normal in the most effective group after four times of injection of recombinant pcDNA3-ALR plasmid. The indexes of PCNA significantly increased in the recombinant pcDNA3-ALR plasmid treatment groups compared to model group. The level of serum AST and ALT remarkably reduced in recombinant pcDNA3-ALR plasmid treatment groups compared to model group. The results showed that the effect of 200 μg/kg recombinant pcDNA3-ALR plasmid in the rats with acute liver injury was stronger than that of 50μg/kg pcDNA3-ALR DNA.The effect of intravenous injection of recombinant pcDNA3ALR plasmid was better. After the rats with acute hepatic failure were treated with recombinant pcDNA3-ALR plasmid,the survival rate (40%) significantly increased in treatment groups compared to control group (15%, P<0.01).CONCLUSION: The ALR gene may play an important role in relieving acute hepatic injury and hepatic failure by promoting hepatic cell proliferation and reducing level ofAST and ALT in CCl4-intoxicated rats.

  9. A reversible and reusable selective chemosensor for fluoride detection using a phenolic OH-containing BODIPY dye by both colorimetric 'naked-eye' and fluorometric modes.

    Science.gov (United States)

    Wang, Lingyun; Fang, Guipo; Cao, Derong

    2014-11-01

    A novel BODIPY-based probe 1 was designed and synthesized as a selective fluorescent and colorimetric chemosensor for fluoride. The spectral responses of 1 to fluoride in acetonitrile were studied: an approximately 118 nm red shift in absorption and 'turn-off' emission response was observed. The striking pink to indigo change in ambient light was thought to be due to the deprotonation of the phenol moiety by way of O-H · · · F hydrogen bonding interactions. Interestingly, when the nonfluorescent 1-F(-) solution treated with trifluoroacetic acid (TFA) resulted in color change from indigo to pink and a significant enhancement of fluorescence intensity (10-fold). Furthermore, the reversibility and reusability of probe 1 for the detection of F(-) ion was tested for four cycles indicating the probe 1 could be used in reversible manner.

  10. Abundance of 13C and 15N in emmer, spelt and naked barley grown on differently manured soils: towards a method for identifying past manuring practice

    DEFF Research Database (Denmark)

    Kanstrup, Marie; Thomsen, Ingrid Kaag; Andersen, Astrid Junker

    2011-01-01

    The shortage of plant-available nutrients probably constrained prehistoric cereal cropping but there is very little direct evidence relating to the history of ancient manuring. It has been shown that the long-term addition of animal manure elevates the δ15N value of soil and of modern crops grown...... on the soil. We have examined the δ15N and δ13C values of soil and of the grain and straw fractions of three ancient cereal types grown in unmanured, PK amended and cattle manured plots of the Askov long-term field experiment. Manure increased biomass yields and the δ15N values of soil and of grain and straw....... Grain weights differed among cereal types but increased in the order: unmanured, PK, and animal manure. The grain and straw total-N concentration was generally not affected by manure addition. Our study suggests that long-term application of manure to permanently cultivated sites would have provided...

  11. Naked by the Pool? Blurring the Image? Ethical Issues in the Portrayal of Young Children in Arts-Based Educational Research

    Science.gov (United States)

    Nutbrown, Cathy

    2011-01-01

    In this article I discuss my growing worry over the use (and nonuse) of photographs of young children in social research. Taking a view of child protection as paramount, I situate the discussion in the context of ethical governance of research, young children as research participants, changing views of childhoods, and the juxtaposition of the Arts…

  12. Subterranean sympatry: an investigation into diet using stable isotope analysis.

    Directory of Open Access Journals (Sweden)

    Gillian N Robb

    Full Text Available In the Western Cape three species of mole-rat occur in sympatry, however, little is known about differences in their dietary preferences. Dietary composition of the three species; the common mole-rat (Cryptomys hottentotus hottentotus, the Cape mole-rat (Georychus capensis and the Cape dune mole-rat (Bathyergus suillus were examined using stable isotope analysis. Blood, fur and claw samples were collected from 70 mole-rats, in addition to several potential food items, to assess food selection of the three species under natural conditions. Overall there was a significant difference in the isotopic composition (δ(13C and δ(15N between all three species and significant differences in their diet composition. There were also significant differences between tissues in all three species suggesting temporal variation in diet. The small size and colonial lifestyle of C. h. hottentotus allows it to feed almost 100% on bulbs, while the solitary and larger species G. capensis and B. suillus fed to a greater extent on other resources such as grasses and clover. B. suillus, the largest of the species, had the most generalized diet. However, overall all species relied most heavily upon geophytes and consumed the same species suggesting competition for resources could exist. We also showed a high level of individual variation in diet choices. This was most pronounced in B. suillus and G. capensis and less so in C. h. hottentotus. We demonstrate that stable isotope analysis can successfully be applied to examine dietary patterns in subterranean mammals and provide insights into foraging patterns and dietary variation at both the inter and intra population level.

  13. Molecular evolution of growth hormone and insulin-like growth factor 1 receptors in long-lived, small-bodied mammals.

    Science.gov (United States)

    Davies, Kalina T J; Tsagkogeorga, Georgia; Bennett, Nigel C; Dávalos, Liliana M; Faulkes, Christopher G; Rossiter, Stephen J

    2014-10-10

    Mammals typically display a robust positive relationship between lifespan and body size. Two groups that deviate markedly from this pattern are bats and African mole-rats, with members of both groups being extremely long-lived given their body size, with the maximum documented lifespan for many species exceeding 20 years. A recent genomics study of the exceptionally long-lived Brandt's bat, Myotis brandtii (41 years), suggested that its longevity and small body size may be at least partly attributed to key amino acid substitutions in the transmembrane domains of the receptors of growth hormone (GH) and insulin-like growth factor 1 (IGF1). However, whereas elevated longevity is likely to be common across all 19 bat families, the reported amino acid substitutions were only observed in two closely related bat families. To test the hypothesis that an altered GH/IGF1 axis relates to the longevity of African mole-rats and bats, we compared and analysed the homologous coding gene sequences in genomic and transcriptomic data from 26 bat species, five mole-rats and 38 outgroup species. Phylogenetic analyses of both genes recovered the majority of nodes in the currently accepted species tree with high support. Compared to other clades, such as primates and carnivores, the bats and rodents had longer branch lengths. The single 24 amino acid transmembrane domain of IGF1R was found to be more conserved across mammals compared to that of GHR. Within bats, considerable variation in the transmembrane domain of GHR was found, including a previously unreported deletion in Emballonuridae. The transmembrane domains of rodents were found to be more conserved, with mole-rats lacking uniquely conserved amino acid substitutions. Molecular evolutionary analyses showed that both genes were under purifying selection in bats and mole-rats. Our findings suggest that while the previously documented mutations may confer some additional lifespan to Myotis bats, other, as yet unknown, genetic

  14. 胫骨裸露皮瓣及肌皮瓣修复91例分析%91 CASES ANALYSIS OF TIBIA NAKED SKIN FLAP AND MUSCULO-CUTANEOUS FLAP PLEROSIS

    Institute of Scientific and Technical Information of China (English)

    张明华; 黄晓元; 龙剑虹; 曾纪章; 周捷

    2002-01-01

    目的:观察胫骨裸露皮瓣及肌皮瓣修复临床效果,探讨其修复适应征.方法:对91例患者,采用邻位皮瓣、带蒂转位肌皮瓣、逆行岛状皮瓣或肌皮瓣、游离皮瓣或肌皮瓣、交腿皮瓣或肌皮瓣移植修复.结果:除2例失败,2例小部分坏死外(失败例改用其他皮瓣修复,部分成活病例给予植皮修复),其余87例皮瓣或肌皮瓣均全部存活,创面一期修复,骨髓炎控制,减少了胫骨坏死.随访1~3年,外观及功能恢复较满意.结论:胫骨髁露临床修复方法较多,恰当选择皮瓣或肌皮瓣修复,有利于创面修复及功能恢复,减少并发症.

  15. 莜麦麸水溶性β-葡聚糖的提取及初步纯化%Extraction and preliminary purification of β -glucan from nake oat (Avena Sativa L)bran

    Institute of Scientific and Technical Information of China (English)

    申瑞玲; 姚惠源; 董吉林

    2004-01-01

    燕麦是我国的古老农作物之一,也是重要的饲草饲料作物。我国种植的主要是大粒裸燕麦种(Avena nuda L)俗称莜麦;少数地区种植普通栽培燕麦(Arena Saliva L)俗称皮燕麦,皮燕麦主要做为马匹的饲料。莜麦是种植区的传统主食,也是饲料的一部分。和其它粮食相比,莜麦不仅蛋白质、脂肪含量高,

  16. Au₂₅(SEt)₁₈, a nearly naked thiolate-protected Au₂₅ cluster: structural analysis by single crystal X-ray crystallography and electron nuclear double resonance.

    Science.gov (United States)

    Dainese, Tiziano; Antonello, Sabrina; Gascón, José A; Pan, Fangfang; Perera, Neranjan V; Ruzzi, Marco; Venzo, Alfonso; Zoleo, Alfonso; Rissanen, Kari; Maran, Flavio

    2014-04-22

    X-ray crystallography has been fundamental in discovering fine structural features of ultrasmall gold clusters capped by thiolated ligands. For still unknown structures, however, new tools capable of providing relevant structural information are sought. We prepared a 25-gold atom nanocluster protected by the smallest ligand ever used, ethanethiol. This cluster displays the electrochemistry, mass spectrometry, and UV-vis absorption spectroscopy features of similar Au25 clusters protected by 18 thiolated ligands. The anionic and the neutral form of Au25(SEt)18 were fully characterized by (1)H and (13)C NMR spectroscopy, which confirmed the monolayer's properties and the paramagnetism of neutral Au25(SEt)18(0). X-ray crystallography analysis of the latter provided the first known structure of a gold cluster protected by a simple, linear alkanethiolate. Here, we also report the direct observation by electron nuclear double resonance (ENDOR) of hyperfine interactions between a surface-delocalized unpaired electron and the gold atoms of a nanocluster. The advantages of knowing the exact molecular structure and having used such a small ligand allowed us to compare the experimental values of hyperfine couplings with DFT calculations unaffected by structure's approximations or omissions.

  17. The practice of naked tablet recognition and tablets splitting in the center pendulum medicine room%我院中心摆药室裸片识别和片剂分劈的实践分析

    Institute of Scientific and Technical Information of China (English)

    刘雅丽; 王志; 王琳; 李欣

    2014-01-01

    目的 探讨裸片识别和片剂分劈在安全用药中的应用.方法 对我院215种裸片进行识别难度调查,对分劈药片进行品种及科室分布调查;交流裸片识别与分劈的经验和技巧.结果 难以识别品种26种,占品种总数的12.09%,绝大多数集中在白色小片和黄浅黄色圆形中片中;经常分劈的品种约50余个,以地高辛分劈使用率最高(分劈使用约70%,整片使用仅30%).各科室分劈频度列前品种多不相同(地高辛分劈度约70%).操作中使摆放品种"单而不裸,混而不乱"可提高用药安全水平,分劈药片时采用比较对照和裹色标记可提高剂量准确性.结论 注重每一个操作细节,才能构筑安全用药的稳固屏障.

  18. An efficient and ultrasensitive rhodamine B-based reversible colorimetric chemosensor for naked-eye recognition of molybdenum and citrate ions in aqueous solution: sensing behavior and logic operation.

    Science.gov (United States)

    Tavallali, Hossein; Deilamy-Rad, Gohar; Parhami, Abolfath; Hasanli, Nahid

    2015-03-15

    In this paper we manifest a novel rhodamine B (RhB) based colorimetric chemosensor for molybdenum and citrate ions (Cit(3-)) in an absolutely aqueous media. It has been identified as highly sensitive probe for Mo(6+) which responds at 4.0 nmol L(-1) concentration levels. RhB while combined with Mo(6+) in aqueous solution displays a color changing from pink to purple which could be quickly dissociated by the addition of citrate in this system so that reversible color changes from purple to pink can be achieved. The comparison of this method with some other methods for citrate indicates that this is the only method which can detect citrate in aqueous solution by color changes. This chemosensor can be applied for quantification of citrate with a linear range covering from 1.67×10(-7) to 1.22×10(-5) M and a detection limit of 2.0×10(-8) M. Moreover, the response of the chemosensor toward Mo(6+) and citrate is fast. In addition, based on above sensing mechanism, an IMPLICATION logic operation can be achieved using Mo(6+) ion and Cit(3-) as the inputs, making RhB a promising candidate for further applications in molecular logic devices and also indicates that RhB is suitable for the detection of Mo(6+) and Cit(3-) ions in real samples. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. O rei está nu: Contrapoder e realização de desejo, na piada e no humor The king is naked: Counter power and wishful thinking in joke and humor

    Directory of Open Access Journals (Sweden)

    Joel Birman

    2010-06-01

    Full Text Available A finalidade deste artigo é a de demonstrar como a piada e o humor têm como alvo privilegiado o campo do poder, sendo assim práticas discursivas eminentemente sociais. Além disso, como formas que seriam de realização de desejo, segundo Freud, seriam ainda formas de desconstrução do poder.The aim of this paper is to demonstrate that joke and humor have power as their privileged target, by which they become social discourse practices. Besides that, as forms of wishful thinking, according to Freud, joke and humor could be a way of deconstructing power.

  20. 肉眼看道德--尼采生命价值论中的自然主义视角%Morals in the Naked Eye--A Naturalistic Vision in Nietzsche's Bio-Axiology

    Institute of Scientific and Technical Information of China (English)

    余虹

    2004-01-01

    "肉眼"象征尼采生命价值论中的自然主义视角,它可分为两个方面:其一,一切价值都来源于肉眼(主人的肉眼和奴隶的肉眼),任何脱离肉眼的"心眼"都是形而上学神学和哲学的编造,一切出自"心眼"的价值(理性价值和神性价值)都是出自肉眼的价值之面具;其二,尼采的全部价值论思考都出自自己的"肉眼",那双善于"转换视觉"但始终坚持"主人立场"的肉眼.文章考察了这双肉眼看道德的洞见与盲视,以及它所引出的富有启示性的问题.

  1. PEG-6000模拟干旱胁迫下裸果木幼苗的生理响应%Physiological Response for PEG-6000 Simulation Naked Fruit Tree Seedlings Under Drought Stress

    Institute of Scientific and Technical Information of China (English)

    孙宗国

    2014-01-01

    Using different concentrations of PEG-6000 simulated drought conditions, the changes of relative water content, praline content, soluble sugar content, MDA content and superoxide dismutase (SOD) activity in leaves on the bare fruit seedling. The result shows that the relative water content of the bare fruit seedling leaves decreased significantly, the proline content increased, the proline accumulation significant when concentration of PEG-6000 is 10% and 15%, soluble sugar content decreased significantly, the content of MDA increased significantly, the drought caused significant damage to membrane system at PEG-6000 stress concentration increases, but the leaf can through enhanced superoxide dismutase (SOD) activity to produce antioxidant protection, relieve oxidative damage . That is, the drought stress caused significant damage to the bare fruit seedlings, and the seedlings also showed a certain drought adaptability.%采用PEG-6000不同浓度模拟干旱条件,研究裸果木幼苗的叶片相对含水量、脯氨酸含量、可溶性糖含量、丙二醛含量、超氧化物歧化酶(SOD)活性的变化。结果表明,随着PEG-6000胁迫浓度的增加,裸果木幼苗叶片的相对含水量显著下降,脯氨酸含量增加。 PEG-6000浓度为10%和15%时脯氨酸显著积累,可溶性糖含量显著降低,丙二醛含量显著增加。干旱对膜系统造成了明显的损伤,但叶片能够通过增强超氧化物歧化酶(SOD)活性产生抗氧化保护,减轻过氧化伤害。即干旱胁迫对裸果木幼苗造成了明显的伤害,而其幼苗也表现出了一定的干旱适应性。

  2. Transition-metal complexes [(PMe(3))(2)Cl(2)M(E)] and [(PMe(3))(2)(CO)(2)M(E)] with naked group 14 atoms (E=C-Sn) as ligands; part 1: parent compounds.

    Science.gov (United States)

    Parameswaran, Pattiyil; Frenking, Gernot

    2009-09-07

    The equilibrium geometries and bond dissociation energies of 16-valence-electron(VE) complexes [(PMe(3))(2)Cl(2)M(E)] and 18-VE complexes [(PMe(3))(2)(CO)(2)M(E)] with M=Fe, Ru, Os and E=C, Si, Ge, Sn were calculated by using density functional theory at the BP86/TZ2P level. The nature of the M--E bond was analyzed with the NBO charge decomposition analysis and the EDA energy-decomposition analysis. The theoretical results predict that the heavier Group 14 complexes [(PMe(3))(2)Cl(2)M(E)] and [(PMe(3))(2)(CO)(2)M(E)] with E=Si, Ge, Sn have C(2v) equilibrium geometries in which the PMe(3) ligands are in the axial positions. The complexes have strong M--E bonds which are slightly stronger in the 16-VE species 1ME than in the 18-VE complexes 2ME. The calculated bond dissociation energies show that the M--E bonds become weaker in both series in the order C>Si>Ge>Sn; the bond strength increases in the order FeE pi-acceptor bonds, which are not degenerate. The shape of the frontier orbitals reveals that the HOMO-2 sigma MO and the LUMO and LUMO+1 pi* MOs of 1ME are very similar to the frontier orbitals of CO.

  3. GAMBARAN TINGKAT PENGETAHUAN, SIKAP DAN PERILAKU NON NAKES TERHADAP FAKTOR RISIKO DAN KOMPLIKASI KEHAMILAN DAN PERSALINAN SEBAGAI UPAYA PENINGKATAN RUJUKAN (Studi di Kabupaten Banjar dan Tanah Laut Provinsi Kalimantan Selatan

    Directory of Open Access Journals (Sweden)

    Rukmini Rukmini

    2012-11-01

    Full Text Available Background: In Indonesia the maternal deaths at rural areas was still high because most deliveries were conducted by non health workers and many occurred homes. Early detection for risk factors, complicated pregnancies and deliveries are one of the efforts to prevent the maternal deaths in community. The purpose of the study was to determine knowledge, attitude and behavior of the non health workers on risk factors, complicated pregnancies and deliveries. Methods: It was an observational study with a cross sectional approach, sample were taken purposively. The study was conducted at four public health center in two district (Banjar and Tanah Laut in South Kalimantan Province. Respondents were non health workers composed of traditional birth attendants (TBAs, Posyandu cadres and families of pregnant and deliveries mothers. Results: The study showed that the majority of respondents had low knowledge on risk factors and complicated pregnancies and deliveries. The majority of respondents, 95% cadres and 83.3% family members were disagree if the delivery services only assisted by health workers. Furthermore, the majority of TBAs (65% agreed that deliveries would be assisted by health workers if they were given some compensation. The majority of the patients would be referred to health facilities if they were found complicated pregnancies and deliveries. Therefore, the knowledge of non health workers has to be improved. So that they have good knowledge and abilities to refer patients rightly and promptly. Key words: the non health workers, knowledge, attitude, behavior, referring, risk factors, complicated pregnancies and deliveries

  4. 局部加压冷疗对控制踝关节镜术后关节肿胀的临床护理%Local Pressure Cold Therapy to Control the Naked Arthroscopy Joint Swelling of Clinical Nursing Research

    Institute of Scientific and Technical Information of China (English)

    车慧虹

    2014-01-01

    目的 探讨局部加压冷疗对控制踝关节镜术后关节肿胀的临床护理效果.方法 选取2013年2月~2014年2月我科采用踝关节镜手术治疗后的患者78例,根据治疗和临床护理方案分为对照组和观察组,各39例.对照组采用常规药物治疗和护理方法.观察组在常规药物治疗和护理的基础上对踝关节镜术后每8h使用加压冷疗系统治疗1次,30min.并观察两组手术后16h、24h、32h、40h、48h患肢的肿胀和疼痛情况,并进行比较.结果 两组疼痛评分及肿胀程度评分在术后16h及24h比较无显著性差异(P>0.05),术后32h、40h、48h观察组明显优于对照组(P<0.05).结论 对踝关节镜术后关节肿胀处采取持续冷敷,能缓解患者踝关节疼痛,减轻肿胀,使其早期进行功能锻炼,有利于踝关节功能早期康复,可降低患者对镇静止痛剂的依赖性.

  5. GETDB: 103903 [GETDB

    Lifescience Database Archive (English)

    Full Text Available 1 Request - muscle epi stripe (strong in naked cuticle), gut fb, mt, gut ubiquit... clusters 1 Stock Request Request - Embryonic Expression muscle Larval GFP epi stripe (strong in naked

  6. Higher Dimensional Radiation Collapse and Cosmic Censorship

    CERN Document Server

    Ghosh, S G

    2000-01-01

    We study the occurrence of naked singularities in the spherically symmetric collapse of radiation shells in a higher dimensional spacetime. The necessary conditions for the formation of a naked singularity or a black hole are obtained. The naked singularities are found to be strong in the Tipler's sense and thus violating cosmic censorship conjecture.

  7. Higher dimensional inhomogeneous dust collapse and cosmic censorship

    CERN Document Server

    Ghosh, S G

    2001-01-01

    We investigate the occurrence and nature of a naked singularity in the gravitational collapse of an inhomogeneous dust cloud described by higher dimensional Tolman-Bondi space-times. The naked singularities are found to be gravitationally strong in the sense of Tipler. Higher dimensions seem to favour black holes rather than naked singularities.

  8. Biomimeitc Design of a Stubble-Cutting Disc Using Finite Element Analysis

    Institute of Scientific and Technical Information of China (English)

    Mo Li; Donghui Chen; Shujun Zhang; Jin Tong

    2013-01-01

    Mole rat (Scaptochirus moschatus),a soil-burrowing mammal,can efficiently dig soil using its fore claws.The profile curves of its claw toe provide excellent structure for digging.In this paper,a biomimetic stubble-cutting disc was designed by learning from the geometrical characteristics of the mole rat claw toes.To compare the structural strength and working efficiency of the biomimetic disc and the conventional stubble-cutting disc,three-dimensional (3D) models of the discs were built and numerical analyzed in ABAQUS.In the dynamic soil cutting model,soil was modeled as an elastic-plastic material with elastic parameters,including Young's modulus,Poisson's ratio and Drucker-Prager criterion,which were obtained from triaxial tests.A general contact algorithm was used to simulate the interaction between rotary disc and soil.In FEA models,for the combined action of normal and friction stresses,the stress on the biomimetic disc is 34.33% lower than that of the conventional disc.For only the normal stress,the stress on the biomimetic disc is 22.64% lower than that on the conventional one.The magnitude of soil stress in biomimetic disc cutting model is 6.87% higher than that in conventional disc.The FEA results indicate that the biomimetic disc performs better in structural strength and cutting efficiency.

  9. Spontaneous expression of magnetic compass orientation in an epigeic rodent: the bank vole, Clethrionomys glareolus

    Science.gov (United States)

    Oliveriusová, Ludmila; Němec, Pavel; Pavelková, Zuzana; Sedláček, František

    2014-07-01

    Magnetoreception has been convincingly demonstrated in only a few mammalian species. Among rodents, magnetic compass orientation has been documented in four species of subterranean mole rats and two epigeic (i.e. active above ground) species—the Siberian hamster and the C57BL/6J mouse. The mole rats use the magnetic field azimuth to determine compass heading; their directional preference is spontaneous and unimodal, and their magnetic compass is magnetite-mediated. By contrast, the primary component of orientation response is learned in the hamster and the mouse, but both species also exhibit a weak spontaneous bimodal preference in the natural magnetic field. To determine whether the magnetic compass of wild epigeic rodents features the same functional properties as that of laboratory rodents, we investigated magnetic compass orientation in the bank vole Clethrionomys glareolus (Cricetidae, Rodentia). The voles exhibited a robust spontaneous bimodal directional preference, i.e. built nests and slept preferentially along the north-south axis, and deflected their directional preference according to a shift in the direction of magnetic north, clearly indicating that they were deriving directional information from the magnetic field. Thus, bimodal, axially symmetrical directional choice seems to be a common feature shared by epigeic rodents. However, spontaneous directional preference in the bank vole appeared to be more pronounced than that reported in the hamster and the mouse. These findings suggest that bank voles are well suited for future studies investigating the adaptive significance and mechanisms of magnetic orientation in epigeic rodents.

  10. The role of host traits, season and group size on parasite burdens in a cooperative mammal.

    Directory of Open Access Journals (Sweden)

    Hermien Viljoen

    Full Text Available The distribution of parasites among hosts is often characterised by a high degree of heterogeneity with a small number of hosts harbouring the majority of parasites. Such patterns of aggregation have been linked to variation in host exposure and susceptibility as well as parasite traits and environmental factors. Host exposure and susceptibility may differ with sexes, reproductive effort and group size. Furthermore, environmental factors may affect both the host and parasite directly and contribute to temporal heterogeneities in parasite loads. We investigated the contributions of host and parasite traits as well as season on parasite loads in highveld mole-rats (Cryptomys hottentotus pretoriae. This cooperative breeder exhibits a reproductive division of labour and animals live in colonies of varying sizes that procreate seasonally. Mole-rats were parasitised by lice, mites, cestodes and nematodes with mites (Androlaelaps sp. and cestodes (Mathevotaenia sp. being the dominant ecto- and endoparasites, respectively. Sex and reproductive status contributed little to the observed parasite prevalence and abundances possibly as a result of the shared burrow system. Clear seasonal patterns of parasite prevalence and abundance emerged with peaks in summer for mites and in winter for cestodes. Group size correlated negatively with mite abundance while it had no effect on cestode burdens and group membership affected infestation with both parasites. We propose that the mode of transmission as well as social factors constrain parasite propagation generating parasite patterns deviating from those commonly predicted.

  11. The amoeboid protists of cryogenic soils in the Kolyma Lowland

    Science.gov (United States)

    Shmakova, L. A.; Fedorov-Davydov, D. G.; Rivkina, E. M.

    2013-12-01

    The taxonomic diversity of amoeboid protists was examined in three types of tundra soils in the Kolyma Lowland, namely, a podzolized podbur, a cryozem, and a gleyzem. In total, 27 naked amoeba genera were identified in the analyzed samples. It was shown that the individual soil types house different communities of naked amoebas. The distribution of naked amoebas in the cryozem had two peaks: one in the upper organic horizon and the other at the boundary with the permafrost.

  12. AcEST: DK962753 [AcEST

    Lifescience Database Archive (English)

    Full Text Available finition sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae Align length 86 Score (bit) ... (bits) Value sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles g......ne-lysine N-methyltransferase HRX OS=Mu... 30 8.3 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anop

  13. AcEST: BP914958 [AcEST

    Lifescience Database Archive (English)

    Full Text Available _id A4ZNR4 Definition sp|A4ZNR4|NKD2_DANRE Protein naked cuticle homolog 2 OS=Dan...nts: (bits) Value sp|A4ZNR4|NKD2_DANRE Protein naked cuticle homolog 2 OS=Danio r...Uroporphyrinogen decarboxylase OS=Synechocy... 30 7.2 >sp|A4ZNR4|NKD2_DANRE Protein naked cuticle homolog 2

  14. Inhomogeneous electromagnetic gravitational collapse

    Energy Technology Data Exchange (ETDEWEB)

    Stein-Schabes, J.A.

    1985-04-15

    The collapse of an inhomogeneous dust cloud in the presence of an electromagnetic field is investigated in detail. The possibility of a naked singularity arising is studied using some known solutions for a spherical charged inhomogeneous dust cloud. It is found that locally naked singularities may develop when the arbitrary functions in the solution are chosen in a special way, but that a global naked singularity will not form. Also the role of the electromagnetic pressure is discussed.

  15. 2-羟基萘甲醛苯甲酰腙衍生物对Al3+的裸眼识别和荧光传感%"Naked-eye" and Fluorescence "Turn-on" Recognition of Al3 + Based on 2-Hydroxy-Naphthalene-Formaldehyde Benzoyl-Hydrazone Derivatives

    Institute of Scientific and Technical Information of China (English)

    陈虹蓉; 伍亚男; 陈仕焰; 马培华; 倪新龙

    2016-01-01

    设计合成了化合物4-羟基苯甲酰-(2-羟基萘甲醛)腙(L)探针,经金属离子识别研究发现探针L对Al3+具有良好的荧光增强识别效果且不受其它金属离子的干扰,且识别过程中溶液的颜色发生了明显变化,可以实现Al3+的可视法检测,核磁滴定、job-plot、基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)等实验结果显示,探针L与Al3+以摩尔比2∶1的方式进行配位.通过对紫外滴定数据的非线性拟合分析,探针L与Al3+的结合常数为1.3×104 L/mol,最低检测限为3.71×10-6 L/mol.因此,该腙类衍生物作为Al3的荧光及比色探针,识别体系线性范围较宽,检测限低,在痕量Al3的检测方面具有潜在的应用价值.

  16. La influencia de la fotografía cinematográfica en el estilo visual. Análisis del estilo de Dick Pope en las obras cinematográficas del director Mike Leigh: Life is sweet, naked, secrets and lies, career girls, topsy-turvy, all or nothing, vera drake Y happy-go-lucky

    OpenAIRE

    Cortés Selva, Laura

    2012-01-01

    La investigación planteada en este trabajo está fundamentada en un conjunto de inquietudes entre las que se encuentra la necesidad de estrechar los vínculos entre la academia y la profesión. En el terreno de la investigación cinematográfica existe un salto cualitativo entre la teoría y la práctica, una escasa reflexión teórica en torno a una disciplina fundamentalmente basada en la praxis y una praxis en la que apenas existe un pensamiento reflexivo. Fruto de esta inquietud nace la necesidad ...

  17. A novel deliver way of gene silencing by renal artery infusion of naked small interference RNA in a rat renal transplant model%经肾动脉灌注细胞间粘附分子—1小片段干扰RNA减轻移植肾缺血再灌注损伤

    Institute of Scientific and Technical Information of China (English)

    罗光恒; 卢一平; 杨立; 宋珺; 马学; 张立元; 李幼平

    2009-01-01

    Objective To investigate the potential preventive role of small interfering RNA targeting ICAM-1 (ICAM-1siRNA) infusion through renal artery in renal transplant cold ischemia injury model. Methods Fisher rats underwent renal isografting. Following removal, the left kidney was preserved in 0 to 4℃ heparin normal saline (NS) solution for 1 h to reinforce the cold ischemia injury. Before transplant, either ICAM-1siRNA (0.1 mg/kg), or (NS) or control siRNA was infused through the renal artery with the renal vein clamped ex vivo. The left kidney was then implanted and the right kidney was removed. At 6, 12, 24, 48 and 72 h post-operation, serum Cr was measured, and the severity of tubular necrosis and inflamed cells infiltration was assessed by histological grading scale respectively. The expression of ICAM-1 mRNA and protein in isograft was detected by real-time RT-PCR and Western blot, respectively. Results Compared with the NS and control siRNA groups, the values of serum Cr in siRNA group were lower at every time point, and the difference after 12 h was statistically significant (P = 0.01~0.001), but there was no significant difference between NS and control siRNA groups (P>0.05). The tubular necrosis and inflamed cells infiltration were less severe in siRNA group than those in NS and control siRNA groups, with the difference being significant especially in 12, 24 and 48 h. After 12 h, the expression levels of ICAM-1 mRNA and protein in the kidney of siRNA group were significantly down-regulated as compared with NS and control siRNA groups, and the difference reached its peak at 24 h. There was no significant difference among 3 groups at 72 h (P>0.05). Conclusion ICAM-1siRNA can significantly prevent and attenuate the renal damage secondary to ischemia by down-regulating the expression of ICAM-1 protein and mRNA.%目的 探讨经肾动脉灌注细胞间粘附分子-1小片段干扰RNA(ICAM-1 siRNA)对移植肾缺血再灌注损伤(IRI)的影响.方法 将Fisher大鼠随机分为3组,接受原位肾移植.供肾在冷保存1 h后,按分组要求分别经肾动脉灌注生理盐水(NS组)、ICAM-1 siRNA(siRNA组)和完全错配siRNA(siRNA错配组),然后进行移植,切除受者自身双肾.分别于移植后6、12、24、48和72 h处死大鼠,测定血清肌酐(Cr),观察移植.肾组织学变化,测定移植肾组织中ICAM-1及其mRNA的表达情况.结果 与NS组和siRNA错配组相比,siRNA组各时点肾小管坏死和炎症细胞浸润程度均较NS组和siRNA错配组有所减轻.siRNA组12、24、48 h时的血Cr值明显低于NS组和siRNA错配组(P=0.01~0.001),尤以24 h时的相差幅度最大.从术后12 h起,siRNA组肾组织中ICAM-1 mRNA的表达明显低于NS组和siRNA错配组(P=0.01~0.001),24 h时的差异达到最大,ICAM-1表达的变化与ICAM-1 mRNA一致.NS组与siRNA错配组相比较,各观察指标的差异均无统计学意义(P>0.05).结论 移植前经肾动脉灌注ICAM-1siRNA可减轻移植肾IRI,其机理可能与下调ICAM-1及其mRNA的表达有关.

  18. Transition-metal complexes [(PMe(3))(2)Cl(2)M(E)] and [(PMe(3))(2)(CO)(2)M(E)] with naked group 14 atoms (E=C-Sn) as ligands; part 2: complexation with W(CO)(5).

    Science.gov (United States)

    Parameswaran, Pattiyil; Frenking, Gernot

    2009-09-07

    Density functional calculations at the BP86/TZ2P level were carried out to understand the ligand properties of the 16-valence-electron(VE) Group 14 complexes [(PMe(3))(2)Cl(2)M(E)] (1ME) and the 18-VE Group 14 complexes [(PMe(3))(2)(CO)(2)M(E)] (2ME; M=Fe, Ru, Os; E=C, Si, Ge, Sn) in complexation with W(CO)(5). Calculations were also carried out for the complexes (CO)(5)W-EO. The complexes [(PMe(3))(2)Cl(2)M(E)] and [(PMe(3))(2)(CO)(2)M(E)] bind strongly to W(CO)(5) yielding the adducts 1ME-W(CO)(5) and 2ME-W(CO)(5), which have C(2v) equilibrium geometries. The bond strengths of the heavier Group 14 ligands 1ME (E=Si-Sn) are uniformly larger, by about 6-7 kcal mol(-1), than those of the respective EO ligand in (CO)(5)W-EO, while the carbon complexes 1MC-W(CO)(5) have comparable bond dissociation energies (BDE) to CO. The heavier 18-VE ligands 2ME (E=Si-Sn) are about 23-25 kcal mol(-1) more strongly bonded than the associated EO ligand, while the BDE of 2MC is about 17-21 kcal mol(-1) larger than that of CO. Analysis of the bonding with an energy-decomposition scheme reveals that 1ME is isolobal with EO and that the nature of the bonding in 1ME-W(CO)(5) is very similar to that in (CO)(5)W-EO. The ligands 1ME are slightly weaker pi acceptors than EO while the pi-acceptor strength of 2ME is even lower.

  19. Urban / Heidi Purga

    Index Scriptorium Estoniae

    Purga, Heidy, 1975-

    2005-01-01

    Uutest heliplaatidest Earth, Wind & Fire "Illumination", Kanye West "Late Registration", Stereo MC's "Paradise", Shaggy "Clothes Drop", Lil Kim "The Naked Truth", Babyface "Grown and Sexy", Sean Paul "Trinity"

  20. Urban / Heidi Purga

    Index Scriptorium Estoniae

    Purga, Heidy, 1975-

    2005-01-01

    Uutest heliplaatidest Earth, Wind & Fire "Illumination", Kanye West "Late Registration", Stereo MC's "Paradise", Shaggy "Clothes Drop", Lil Kim "The Naked Truth", Babyface "Grown and Sexy", Sean Paul "Trinity"

  1. Organization of the Mammalian Ionome According to Organ Origin, Lineage Specialization, and Longevity

    Directory of Open Access Journals (Sweden)

    Siming Ma

    2015-11-01

    Full Text Available Trace elements are essential to all mammals, but their distribution and utilization across species and organs remains unclear. Here, we examined 18 elements in the brain, heart, kidney, and liver of 26 mammalian species and report the elemental composition of these organs, the patterns of utilization across the species, and their correlation with body mass and longevity. Across the organs, we observed distinct distribution patterns for abundant elements, transition metals, and toxic elements. Some elements showed lineage-specific patterns, including reduced selenium utilization in African mole rats, and positive correlation between the number of selenocysteine residues in selenoprotein P and the selenium levels in liver and kidney across mammals. Body mass was linked positively to zinc levels, whereas species lifespan correlated positively with cadmium and negatively with selenium. This study provides insights into the variation of mammalian ionome by organ physiology, lineage specialization, body mass, and longevity.

  2. Animal Communications Through Seismic Vibrations

    Energy Technology Data Exchange (ETDEWEB)

    Hill, Peggy (University of Tulsa)

    2001-05-02

    Substrate vibration has been important to animals as a channel of communication for millions of years, but our literature on vibration in this context of biologically relevant information is only decades old. The jaw mechanism of the earliest land vertebrates allowed them to perceive substrate vibrations as their heads lay on the ground long before airborne sounds could be heard. Although the exact mechanism of vibration production and the precise nature of the wave produced are not always understood, recent development of affordable instrumentation to detect and measure vibrations has allowed researchers to answer increasingly sophisticated questions about how animals send and receive vibration signals. We now know that vibration provides information used in predator defense, prey detection, recruitment to food, mate choice, intrasexual competition, and maternal/brood social interactions in a variety of insect orders, spiders, crabs, scorpions, chameleons, frogs, golden moles, mole rats, kangaroos rats, wallabies, elephants and bison.

  3. [Morphological diversity in the postnatal skull development in representatives of two families of rodents (Spalacidae, Castoridae, Rodentia)].

    Science.gov (United States)

    Puzachenko, A Iu; Korablev, N P

    2014-01-01

    This is the first study to describe the results of measurement of three information parameters of morphological diversity (entropy, the measure of organization, and the Kullback-Leibler divergence) in the course of postnatal development of the skull in the populations of two rodent species (greater mole rat (Spalax microphthalmus Guld.) and Eurasian beaver (Castor fiber (L.)). The terms "morphosystem" and "morphological space" and its structure are introduced. Within the framework of the developed approach, "morphological diversity" is considered as a variable associated with the morphological space structure. Testing the hypothesis of the dominance of self-organization processes and an increase in the organization of the morphological diversity of the skull in the course ofontogeny showed its inconsistency. The morphosystem of the skull of the studied species undergoes transitions between more organized and less organized states, periodically approaching and departing from the "steady state." Such dynamics characterizes the morphosystem of the skull as a dynamic and nonlinear system.

  4. Environmentally Compliant Coating Remover Evaluation

    Science.gov (United States)

    2012-08-30

    310AG, RPM Technology • Desolift 5269, PPG • Turco 6813E, Henkel • DePaint XP, Aerochem, Inc. • Plane Naked, Aerochem, Inc • DeKote AF, Aerochem...the graphite epoxy panels for any of the removers 17 Control DeKote Desolift 5269 PolyGone 310 AG Gel Turco 6813E DePaint XP Plane Naked

  5. The study of gravitational collapse model in higher dimensional space-time

    CERN Document Server

    Debnath, U; Debnath, Ujjal; Chakraborty, Subenoy

    2003-01-01

    We investigate the end state of the gravitational collapse of an inhomogeneous dust cloud in higher dimensional space-time. The naked singularities are shown to be developing as the final outcome of non-marginally bound collapse. The naked singularities are found to be gravitationally strong in the sense of Tipler .

  6. Collapsing perfect fluid in self-similar five dimensional space-time and cosmic censorship

    CERN Document Server

    Ghosh, S G; Saraykar, R V

    2014-01-01

    We investigate the occurrence and nature of naked singularities in the gravitational collapse of a self-similar adiabatic perfect fluid in a five dimensional space-time. The naked singularities are found to be gravitationally strong in the sense of Tipler and thus violate the cosmic censorship conjecture.

  7. AcEST: DK960945 [AcEST

    Lifescience Database Archive (English)

    Full Text Available homeobox protein Rx-A OS=Xenopus lae... 32 2.8 sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anophele...PPPYQCGANF 288 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae GN=nkd PE=2 SV=1 Leng

  8. AcEST: BP912901 [AcEST

    Lifescience Database Archive (English)

    Full Text Available e subunit beta OS... 30 6.8 sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles g... 30 6.8 sp|Q1...PLLCRTRNLNFASNSLLF 1338 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae GN=nkd PE=2

  9. AcEST: BP918983 [AcEST

    Lifescience Database Archive (English)

    Full Text Available S=Homo sapiens GN=... 32 1.6 sp|Q9VVV9|NKD_DROME Protein naked cuticle OS=Drosophila melanoga... 32 1.6 sp|Q...VIANSGLIALVTFVVLLLSYVF 218 >sp|Q9VVV9|NKD_DROME Protein naked cuticle OS=Drosophila melanogaster GN=nkd PE=1

  10. AcEST: DK962109 [AcEST

    Lifescience Database Archive (English)

    Full Text Available nal homeobox protein Rx-A OS=Xenopus lae... 32 2.8 sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anop...GAMGPPPPYQCGANF 288 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae GN=nkd PE=2 SV=1

  11. AcEST: DK946761 [AcEST

    Lifescience Database Archive (English)

    Full Text Available cium-dependent protein kinase 1 OS=Arab... 27 8.9 sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anoph...EEIAGLKEMFNMI 463 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae GN=nkd PE=2 SV=1 L

  12. Dicty_cDB: SSK246 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available smodium falciparum DNA from MA... 32 5.2 (A4ZNR4) RecName: Full=Protein naked cuticle homolog 2; ... 32 8.9 ...(A4ZNR5) RecName: Full=Protein naked cuticle homolog 2-like; ... 32 8.9 protein update 2009. 6.14 PSORT psg:

  13. AcEST: DK955739 [AcEST

    Lifescience Database Archive (English)

    Full Text Available .2 sp|O42567|RXB_XENLA Retinal homeobox protein Rx-B OS=Xenopus lae... 32 1.6 sp|Q08AA9|NKD_ANOGA Protein naked...HALQPLGAMGPPPPYQCGANY 288 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae GN=nkd PE=

  14. 77 FR 17479 - Star Pipe Products, Ltd.; Analysis of Proposed Consent Order To Aid Public Comment

    Science.gov (United States)

    2012-03-26

    ..., and must identify the specific portions of the comment to be withheld from the public record. See FTC... 2008 price restraints among Star, McWane, and Sigma alleged in the complaint are naked restraints on... per se condemnation of naked restraints has been affected by anything said either in California Dental...

  15. AcEST: BP916193 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 12 OS=Ara... 30 4.9 sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anophel...WIVDDKVAPDKPL 291 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae GN=nkd PE=2 SV=1 L

  16. AcEST: DK951808 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ein Rx-A OS=Xenopus lae... 32 2.2 sp|Q08AA9|NKD_ANOGA Protein naked cuticle homol...PG P PF+ S LQ G PPPP G + Sbjct: 246 TTPPSLPGSYTPPPFINPVSVGHALQPLGAMGPPPPYQCGANF 288 >sp|Q08AA9|NKD_ANOGA Protein naked

  17. AcEST: DK952672 [AcEST

    Lifescience Database Archive (English)

    Full Text Available E1_CAVPO Endothelin-converting enzyme 1 OS=Cavia por... 31 5.1 sp|Q29DJ1|NKD_DROPS Protein naked cuticle OS=...>sp|Q29DJ1|NKD_DROPS Protein naked cuticle OS=Drosophila pseudoobscura pseudoobsc

  18. AcEST: BP919050 [AcEST

    Lifescience Database Archive (English)

    Full Text Available in-like arabinogalactan protein 15 ... 31 4.5 sp|Q2TJA6|NKD1_DANRE Protein naked cuticle homolog 1 OS=Danio ...uery: 236 YVHISYH 216 ++H H Sbjct: 252 FIHTLLH 258 >sp|Q2TJA6|NKD1_DANRE Protein naked

  19. AcEST: BP914047 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 4 member A OS=... 30 3.8 sp|Q6ZT46|YK027_HUMAN Putative uncharacterized protein FLJ44967 ... 29 6.5 sp|Q08AA9|NKD_ANOGA Protein nake...SMSSPRGLSKSSPRGLSKSSPWGLSKSFLCR 208 >sp|Q08AA9|NKD_ANOGA Protein naked cuticle homolog OS=Anopheles gambiae

  20. Dicty_cDB: VSC555 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available um DNA from MA... 32 5.5 (A4ZNR4) RecName: Full=Protein naked cuticle homolog 2; ...... 32 9.3 (A4ZNR5) RecName: Full=Protein naked cuticle homolog 2-like; ... 32 9.3 protein update 2009. 3.20