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Sample records for mycoplasma pneumoniae chlamydophila

  1. A Multiplex PCR for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in Clinical Specimens

    Science.gov (United States)

    2007-11-02

    NAVAL HEALTH RESEARCH CENTER A MULTIPLEX PCR FOR DETECTION OF Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, AND Bordetella...5300 2 A Multiplex PCR for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in Clinical... Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in uncultured patient specimens. These organisms cause similar symptomologies

  2. Mycoplasma Pneumoniae and Chlamydophila pneumoniae in hospitalized children with bronchiolitis.

    Science.gov (United States)

    Zirakishvili, D; Chkhaidze, I; Barnabishvili, N

    2015-03-01

    Bronchiolitis is an acute lower respiratory tract infection in early childhood caused mainly by different viruses. Etiology of bronchiolitis have been studied in different environments and populations. Respiratory syncytial virus (RSV), human Metapneumovirus (hMPV), human Bocavirus (hBoV), human Rhinoviruses (hRV) have consistently been shown to predominate. Few studies however have attempted to determine whether other pathogens, particularly Mycoplasma Pneumoniae (MP) and Chlamydophila pneumoniae (CP), are associated with bronchiolitis in children under 2 years of age. The aim of this study was to determine the prevalence and clinical features of MP and CP in children under the age of 2 years presenting to the Iashvili Central Children Hospital in Tbilisi with various severities and clinical manifestations of bronchiolitis. Acute and convalescent serum samples were tested by ELISA for IgM and IgG antibodies to RSV, CP and MP.37 children under two years of age were studied. In 19 patients out of 37 (51.35%) etiological diagnosis were established and in 18 patients (48.65%) no pathogens were found. 11 patients (29.72%) had either CP or MP and 8 patients (21.62%) had RSV. Children infected with CP and MP had less severe bronchiolitis than those infected with RSV. Co-infection was not associated with disease severity. There were no statistically significant differences between groups with respect to length of hospital stay. Our study underlines the importance of atypical bacterial pathogens in acute bronchiolitis in children under 2 years and highlights the complex epidemiology and clinical features of these pathogens in this age group.

  3. Mycoplasma pneumonia

    Science.gov (United States)

    Walking pneumonia; Community-acquired pneumonia - mycoplasma; Community-acquired pneumonia - atypical ... Mycoplasma pneumonia usually affects people younger than 40. People who live or work in crowded areas such as schools ...

  4. Infections caused by Chlamydophila pneumoniae.

    Science.gov (United States)

    Choroszy-Król, Irena; Frej-Mądrzak, Magdalena; Hober, Martyna; Sarowska, Jolanta; Jama-Kmiecik, Agnieszka

    2014-01-01

    High affinity to the epithelial lining of the airways makes Chlamydophila pneumoniae a common etiological agent of respiratory tract infections (RTI). It causes among others: pharyngitis, tracheitis, sinusitis, otitis media, bronchitis and bronchiolitis, and pneumonia. It is estimated that Chlamydophila pneumoniae infection is responsible for about 20% of lower respiratory tract infections. Chlamydophila pneumoniae infection may play an important role in the pathogenesis and course of bronchial asthma. The recent results indicate that Chlamydophila pneumoniae infection may be a factor responsible for 4-16% of COPD (Chronic obstructive pulmonary disease) exacerbations. A relationship of chlamydial infection with atherosclerosis raises huge interest. A connection of Chlamydophila pneumoniae infection with other non-communicable diseases such as lung cancer, arthritis, Alzheimer's disease, multiple sclerosis, sarcoidosis and erythema nodosum is also recognized, although the role of these bacteria has not been fully understood in any of the listed diseases.

  5. Atypical pneumonia

    Science.gov (United States)

    ... people younger than age 40. Pneumonia due to Chlamydophila pneumoniae bacteria occurs year round. Pneumonia due to ... Pneumonia due to mycoplasma and chlamydophila bacteria is usually ... gets worse during the first 4 to 6 days, and then improves over ...

  6. Chlamydophila pneumoniae Infection Induced Nodular Vasculitis

    Directory of Open Access Journals (Sweden)

    H. Sakuma

    2011-12-01

    Full Text Available We present the case history of a 48-year-old male patient with Chlamydophila (Chlamydia pneumoniae who developed a nodular vasculitis. He developed a cutaneous vasculitis with the onset of respiratory symptoms. The diagnosis of Chlamydophila pneumoniae infection was based on serology. Since this infection is very common in our population, although often asymptomatic, it should be systematically considered as a causative agent of nodular vasculitis.

  7. A Compendium for Mycoplasma pneumoniae.

    Science.gov (United States)

    Parrott, Gretchen L; Kinjo, Takeshi; Fujita, Jiro

    2016-01-01

    Historically, atypical pneumonia was a term used to describe an unusual presentation of pneumonia. Currently, it is used to describe the multitude of symptoms juxtaposing the classic symptoms found in cases of pneumococcal pneumonia. Specifically, atypical pneumonia is a syndrome resulting from a relatively common group of pathogens including Chlamydophila sp., and Mycoplasma pneumoniae. The incidence of M. pneumoniae pneumonia in adults is less than the burden experienced by children. Transmission rates among families indicate children may act as a reservoir and maintain contagiousness over a long period of time ranging from months to years. In adults, M. pneumoniae typically produces a mild, "walking" pneumonia and is considered to be one of the causes of persistent cough in patients. M. pneumoniae has also been shown to trigger the exacerbation of other lung diseases. It has been repeatedly detected in patients with bronchitis, asthma, chronic obstructive pulmonary disorder, and cystic fibrosis. Recent advances in technology allow for the rapid diagnosis of M. pneumoniae through the use of polymerase chain reaction or rapid antigen tests. With this, more effort has been afforded to identify the causative etiologic agent in all cases of pneumonia. However, previous practices, including the overprescribing of macrolide treatment in China and Japan, have created increased incidence of macrolide-resistant M. pneumoniae. Reports from these countries indicate that >85% of M. pneumoniae pneumonia pediatric cases are macrolide-resistant. Despite its extensively studied past, the smallest bacterial species still inspires some of the largest questions. The developments in microbiology, diagnostic features and techniques, epidemiology, treatment and vaccines, and upper respiratory conditions associated with M. pneumoniae in adult populations are included within this review.

  8. A compendium for Mycoplasma pneumoniae

    Directory of Open Access Journals (Sweden)

    Gretchen Lynn Parrott

    2016-04-01

    Full Text Available Historically, atypical pneumonia was a term used to describe an unusual presentation of pneumonia. Currently, it is used to describe the multitude of symptoms juxtaposing the classic symptoms found in cases of pneumococcal pneumonia. Specifically, atypical pneumonia is a syndrome resulting from a relatively common group of pathogens including Chlamydophila sp., and Mycoplasma pneumoniae. The incidence of M. pneumoniae pneumonia in adults is less than the burden experienced by children. Transmission rates among families indicate children may act as a reservoir and maintain contagiousness over a long period of time ranging from months to years. In adults, M. pneumoniae typically produces a mild, walking pneumonia and is considered to be one of the causes of persistent cough in patients. M. pneumoniae has also been shown to trigger the exacerbation of other lung diseases. It has been repeatedly detected in patients with bronchitis, asthma, chronic obstructive pulmonary disorder and cystic fibrosis. Recent advances in technology allow for the rapid diagnosis of M. pneumoniae through the use of polymerase chain reaction (PCR or rapid antigen tests. With this, more effort has been afforded to identify the causative etiologic agent in all cases of pneumonia. However, previous practices, including the overprescribing of macrolide treatment in China and Japan, have created increased incidence of macrolide-resistant M. pneumoniae. Reports from these countries indicate that >85% of M. pneumoniae pneumonia pediatric cases are macrolide-resistant. Despite its extensively studied past, the smallest bacterial species still inspires some of the largest questions. The developments in microbiology, diagnostic features and techniques, epidemiology, treatment and vaccines, and upper respiratory conditions associated with M. pneumoniae in adult populations are included within this review.

  9. Psychosis following mycoplasma pneumonia.

    Science.gov (United States)

    Banerjee, Bonita; Petersen, Kyle

    2009-09-01

    Extrapulmonary manifestations of Mycoplasma pneumoniae are well described, including a subset of central nervous system (CNS)-associated syndromes. In pediatric populations, frequencies of CNS sequelae occur in 0.1% to 7% of patients. Neurologic illness associated with M. pneumoniae, such as meningitis, encephalitis, polyradiculitis, Guillain-Barre, and stroke have been reported; however, the incidence of M. pneumoniae-associated organic brain syndrome is rare. We present the case of a 20-year-old midshipman with acute psychosis following resolution of M. pneumoniae pneumonia and review 6 other adult cases found in the literature. M. pneumoniae remains one of the most common causes of respiratory illnesses in the military recruit setting and therefore should always be suspected as an organic cause of mental status changes in young persons such as recruits, cadets, and midshipmen particularly with antecedent respiratory illnesses.

  10. Chlamydophila pneumoniae infection and cardiovascular disease

    Directory of Open Access Journals (Sweden)

    Rajnish Joshi

    2013-01-01

    Full Text Available Atherosclerosis is a multifactorial vascular inflammatory process; however, the inciting cause for inflammation remains unclear. Two decades ago, Chlamydophila pneumoniae (formerly Chlamydia pneumoniae infection was proposed as a putative etiologic agent. We performed a PubMed search using the keywords Chlamydia and atherosclerosis in a Boolean query to identify published studies on C. pneumoniae and its role in atherogenesis, and to understand research interest in this topic. We found 1,652 published articles on this topic between 1991 and 2011. We analyzed relevant published studies and found various serological, molecular, and animal modeling studies in the early period. Encouraged by positive results from these studies, more than a dozen antibiotic clinical-trials were subsequently conducted, which did not find clinical benefits of anti-Chlamydophila drug therapy. While many researchers believe that the organism is still important, negative clinical trials had a similar impact on overall research interest. With many novel mechanisms identified for atherogenesis, there is a need for newer paradigms in Chlamydophila-atherosclerosis research.

  11. Detection of Chlamydophila pneumoniae antigens in patients with chronic cough.

    Science.gov (United States)

    Choroszy-Krol, Irena; Frej-Madrzak, Magdalena; Jama-Kmiecik, Agnieszka; Sarowska, Jolanta; Serek, Pawel; Pirogowicz, Iwona; Nittner-Marszalska, Marita

    2013-01-01

    The aim of this study was to analyze the rate of Chlamydophila pneumoniae infection in adults with symptoms of chronic cough. The study was conducted in 83 hospitalized patients aged 18-67 suffering of chronic cough. The control group consisted of 20 healthy age-matched subjects without any respiratory symptoms. Bacteriological tests on the presence of Chlamydophila pneumoniae antigen were performed in throat swabs by indirect immunofluorescence technique using monoclonal antibodies labeled with fluorescein isothiocyanate. The rate of Chlamydophila infected patients was examined in relation to age and gender. The Chlamydophila pneumoniae antigen was detected in 15 (18 %) out of the 83 patients; about equally in both genders. Furthermore, we found that the patients aged 28-37 constituted the age group that most frequently tested positive for Chlamydophila pneumoniae. Unraveling the presence of Chlamydia infection in chronic cough patients enables to introduce a timely implementation of effective therapy and thus can prevent distant complications.

  12. Association of Alzheimer's disease and Chlamydophila pneumoniae.

    Science.gov (United States)

    Stallings, Tiffany L

    2008-06-01

    This paper critically reviews the association of infection by Chlamydophila pneumoniae (C. pneumoniae) and Alzheimer's disease (AD). The aging population has increased interest in finding the cause of AD, but studies have yielded contradictory results that are likely due to varying diagnostic tools and different uses of diagnostic tests. Knowledge of AD's characteristics, risk factors, and hypothesized etiologies has expanded since Alois Alzheimer's initial description of AD. Epidemiologic and projection studies provide incidence estimates of AD through a two-stage method: (1) primary diagnosis of dementia by cognitive testing such as Mini-Mental State Examination (MMSE), and (2) clinical diagnosis of AD through criteria such as National Institute of Neurological and Communicative Diseases and Stroke/Alzheimer's Disease and Related Disorders Association (NINCDS-ADRDA). Cross-sectional studies yield prevalence estimates of infection by C. pneumoniae by detecting immunoglobulins through laboratory tests such as microimmunofluorescence (MIF). Studies examining the association of C. pneumoniae and AD are limited, but brain autopsy provides information about presence, proximity to areas associated with AD, and bacterial load. Standardization of diagnostic techniques would allow for better comparability of studies, but uncertainty about the best method of diagnosis of infection by C. pneumoniae and AD may call for revised or novel diagnostic tools.

  13. Three cases of atypical pneumonia caused by Chlamydophila psittaci.

    Science.gov (United States)

    Chau, S; Tso, Eugene Y K; Leung, W S; Fung, Kitty S C

    2015-06-01

    Psittacosis is a zoonotic disease caused by Chlamydophila psittaci. The most common presentation is atypical pneumonia. Three cases of pneumonia of varying severity due to psittacosis are described. All patients had a history of avian contact. The diagnosis was confirmed by molecular detection of Chlamydophila psittaci in respiratory specimens. The cases showed good recovery with doxycycline treatment. Increased awareness of psittacosis can shorten diagnostic delay and improve patient outcomes.

  14. Enzymatic characterization of Chlamydophila pneumoniae phospholipase D.

    Science.gov (United States)

    Mancini, Fabiola; Ciervo, Alessandra

    2015-01-01

    Chlamydophila pneumoniae, an aetiological agent of respiratory infection, is also thought to play an immuno-pathogenetic role in atherosclerosis by contributing to inflammation and plaque instability. Phospholipase D (PLD) is an enzyme involved in lipid metabolism and may have a direct or indirect impact on virulence and the inflammatory response. Some aspects of the developmental cycle of C. pneumoniae suggest a direct implication of its PLD (CpPLD) in the pathogenesis, specifically by affecting the regulation of lipid metabolism and lipid exchange between C. pneumoniae and host cells. Our previous studies disclosed a specific anti-CpPLD antibody response in patients with acute coronary syndromes chronically infected with C. pneumoniae, and demonstrated that this antigen is a factor able to drive the inflammatory process in atherosclerosis. Due to the intriguing aspects of the CpPLD, the present study investigated CpPLD enzymatic activity of the protein and the two domains that include one HKD motif each polypeptide. Our results showed that CpPLD was able to synthesize the cardiolipin (CL) but unable to hydrolyze phospholipids. It was also observed that each single HKD motif has an independent CL synthetase activity. This enzymatic activity of CpPLD could be important in the inflammatory process within the atherothrombotic events.

  15. Pneumonia Outbreak Caused by Chlamydophila pneumoniae Among US Air Force Academy Cadets, Colorado, USA

    Science.gov (United States)

    2015-06-01

    Article 3. DATES COVERED (From – To) Sep 2013 – May 2014 4. TITLE AND SUBTITLE Pneumonia Outbreak Caused by Chlamydophila pneumoniae among US Air Force...October 2013–May 2014, there were 102 cases of pneumonia diagnosed in US Air Force Academy cadets. A total of 73% of tested nasal washes contained...Chlamydophila pneumoniae . This agent can be considered to be present on campus settings during outbreaks with numerous, seemingly disconnected cases of

  16. Molecular evidence of Chlamydophila pneumoniae infection in reptiles in Argentina.

    Science.gov (United States)

    Frutos, María C; Monetti, Marina S; Ré, Viviana E; Cuffini, Cecilia G

    2014-01-01

    In the central area of Argentina, the epidemiological and molecular characteristics of Chlamydophila pneumoniae infections in reptiles are still unknown. A nested polymerase chain reaction of the rpoB gene was used to detect C. pneumoniae in cloacal swab samples from 19 reptiles at a recreational area. Eleven (57.89%) reptiles were positive; the sequencing and phylogenetic analysis confirmed the presence of this bacterium. Neither C. pneumoniae DNA in the caregivers pharynges nor IgM antibodies anti-C. pneumoniae in their serum samples were detected; however, caregivers presented very high titers of IgG anti-C. pneumoniae. The detection of C. pneumoniae DNA in reptiles demonstrated the circulation of this agent in the recreational area and could be responsible for the exacerbated immune response of the personnel handling the reptiles, which suggests a potential zoonotic cycle. This is the first report of the detection of C. pneumoniae in reptiles in Argentina.

  17. Antibody responses of Chlamydophila pneumoniae pneumonia: Why is the diagnosis of C. pneumoniae pneumonia difficult?

    Science.gov (United States)

    Miyashita, Naoyuki; Kawai, Yasuhiro; Tanaka, Takaaki; Akaike, Hiroto; Teranishi, Hideto; Wakabayashi, Tokio; Nakano, Takashi; Ouchi, Kazunobu; Okimoto, Niro

    2015-07-01

    The ELNAS Plate Chlamydophila pneumoniae commercial test kit for the detection of anti-C. pneumoniae-specific immunoglobulin M (IgM), IgA and IgG antibodies has become available in Japan recently. To determine the optimum serum collection point for the ELNAS plate in the diagnosis of C. pneumoniae pneumonia, we analyzed the kinetics of the antibody response in patients with laboratory-confirmed C. pneumoniae pneumonia. We enrolled five C. pneumoniae pneumonia cases and collected sera from patients for several months. The kinetics of the IgM and IgG antibody responses were similar among the five patients. Significant increases in IgM and IgG antibody titer between paired sera were observed in all patients. IgM antibodies appeared approximately 2-3 weeks after the onset of illness, reached a peak after 4-5 weeks, and were generally undetectable after 3-5 months. IgG antibodies developed slowly for the first 30 days and reached a plateau approximately 3-4 months after the onset of illness. The kinetics of IgA antibody responses were different among the five patients, and significant increases in IgA antibody titer between paired sera were observed in only two patients. Although the sample size was small, the best serum collection time seemed to be approximately 3-6 weeks after onset of illness when using a single serum sample for the detection of IgM antibodies. Paired sera samples should be obtained at least 4 weeks apart. IgA antibody analysis using ELNAS may not be a useful marker for acute C. pneumoniae pneumonia.

  18. Cytotoxicity of Mycoplasma pneumoniae Membranes

    Science.gov (United States)

    Gabridge, Michael G.; Johnson, Cynthia K.; Cameron, Alexander M.

    1974-01-01

    Organ cultures of adult hamster trachea were used to evaluate the cytotoxic potential of cell fractions of Mycoplasma pneumoniae. Cytoplasm was essentially devoid of activity, whereas viable cells and membrane preparations, at a level of 25 μg of protein per ml, induced necrosis. Damage, as revealed by light and electron microscopy, included ciliostasis, vacuolization, loss of ciliated respiratory epithelial cells, disorganization, and a loss of polarity. Dose response data indicated that the speed and degree of cytotoxicity was directly related to the concentration of membranes. Doses of 30 to 60 μg of protein per ml could reduce relative ciliary activity to 20% of the control level within 4 days. Membranes prepared after freeze-thaw lysis of cells were almost twice as active as those isolated after a combination of osmotic and sonic shock. Membranes of M. fermentans were inactive, though both the FH and M129 strains of M. pneumoniae were toxic. These data indicate that the toxic factor responsible for M. pneumoniae may be located in the cell membrane. Images PMID:16558100

  19. [Mycoplasma Pneumoniae-Induced Meningoencephalitis].

    Science.gov (United States)

    Özel, C; Dafotakis, M; Nikoubashman, O; Litmathe, J; Matz, O; Schöne, U

    2015-07-01

    In clinical practice, secondary infections of the central nervous system (CNS) represent rare yet severe complications of their respective primary infections. In this case report, we describe a 22-year-old patient with a medical history of Asthma bronchiale, who developed significant neurological deficits after a respiratory infection. The neurological symptoms progressed despite antibiotic therapy with vancomycin, ampicillin and ceftriaxone. The patient's cerebrospinal fluid and a cranial magnetic resonance imaging (MRI) furnished evidence of acute meningoencephalitis. Microbiological assessment confirmed an acute mycoplasma pneumonia infection. Changing the patient's antibiotic regimen to minocycline and prednisolone led to significant clinical improvement. Pathomechanisms and therapeutic options to treat meningoencephalitis will be discussed in the following. © Georg Thieme Verlag KG Stuttgart · New York.

  20. Liver granulomatosis: a case of Chlamydophila pneumoniae infection.

    Science.gov (United States)

    Yildiz, H; Wieërs, G; Yombi, J C; Marot, J C

    2015-02-01

    An 18-year-old man was referred to the Internal Medicine ward because of a 2-week history of intermittent high fever, weight loss and cough. Clinical examination revealed hepato-splenomegaly and multiple lymph nodes swelling while laboratory tests showed elevated C-reactive protein, gamma glutamyl transferase and lactate dehydrogenase. All serologic testes for auto-immune antibodies, viruses and bacteria were negative except for Chlamydophila pneumoniae. An 18-FDG PET computed tomography scanner showed hypermetabolism in the liver, spleen and lymph nodes. We therefore conducted a liver biopsy that demonstrated non-necrotizing granulomas. We conclude to a C. pneumoniae infection associated with a granulomatous hepatitis. After treatment with Doxyciclin the patient had no more fever, hepatosplenomegaly resolved and blood testes normalized. This case report is to our knowledge the first report of a granulomatous hepatitis associated with C. pneumoniae respiratory infection.

  1. Mycoplasma contamination of Chlamydia pneumoniae isolates

    DEFF Research Database (Denmark)

    Huniche, BS; Jensen, Lise Torp; Birkelund, Svend

    1998-01-01

    We examined 6 C. pneumonia isolates from The American Type Culture Collection (ATCC) and 2 Finnish isolates for Mycoplasma contamination. Three of the ATCC isolates and both of the Finnish isolates were Mycoplasma-contaminated. The contaminants were characterized by means of growth in BEa and BEg...... media, immunoblotting, polymerase chain reaction and pulsed field gel electrophoresis. Two of the 6 ATCC isolates [ATCC VR1355 (TWAR strain 2043) and ATCC VR1356 (TWAR strain 2023)] were infected with Mycoplasma hominis and 1 isolate [ATCC VR2282 (TWAR strain TW183)] was contaminated with both...... Mycoplasma hominis and Mycoplasma orale, whereas 3 of the ATCC isolates [ATCC VR1310, ATCC VR1360 (TWAR strain CM-1) and ATCC 53592 (TWAR strain AR39)] were not contaminated. The Finnish C. pneumoniae isolates Kajaani 6 and Parola were found to be contaminated with M. hominis and M. orale, respectively...

  2. A Cluster of Legionella-Associated Pneumonia Cases in a Population of Military Recruits

    Science.gov (United States)

    2007-06-01

    pneumophila, Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Bordetella pertussis (8). This test was applied retrospectively to 240 samples... Chlamydophila ) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and its real-time counterpart. J. Clin. Microbiol. 43:565–571...8. McDonough, E. A., C. P. Barrozo, K. L. Russell, and D. Metzgar. 2005. A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila

  3. Ekstrapulmonale komplikationer ved mycoplasma pneumoniae-infektioner

    DEFF Research Database (Denmark)

    Bjørn, Anne-Mette Bay; Lebech, Anne-Mette K

    2002-01-01

    Mycoplasma pneumoniae is a common cause of atypical pneumonia in children and young adults. The infection is generally mild and only a very few patients are admitted to hospital. However, extrapulmonary complications are well recognised--mostly as manifestations from the central nervous system (CNS)....

  4. Atherosclerosis Induced by Chlamydophila pneumoniae: A Controversial Theory

    Directory of Open Access Journals (Sweden)

    Hamidreza Honarmand

    2013-01-01

    Full Text Available More than a century ago, inflammation and infection were considered to have atherogenic effects. The old idea that coronary heart disease (CHD possibly has an infectious etiology has only reemerged in recent years. Atherosclerosis is the main pathological process involved in CHD and is, logically, the first place to look for infectious etiology. The process of atherosclerosis itself provides the first hints of potential infectious cause. Smooth muscle proliferation, with subsequent intimal thickening, luminal narrowing, and endothelial degeneration, constitutes the natural history of atherosclerosis, being with the severity and speed of these changes. Both viral and bacterial pathogens have been proposed to be associated with the inflammatory changes found in atherosclerosis. Recently, Chlamydophila pneumoniae (C. pneumoniae has been implicated as a possible etiologic agent of coronary artery disease and atherosclerosis. New evidence which supports a role for C. pneumoniae in the pathogenesis of atherosclerosis has emerged. C. pneumoniae has been detected in atherosclerotic arteries by several techniques, and the organism has been isolated from both coronary and carotid atheromas. Recent animal models have suggested that C. pneumoniae is capable of inducing atherosclerosis in both rabbit and mouse models of atherosclerosis. Furthermore, human clinical treatment studies which examined the use of antichlamydial macrolide antibiotics in patients with coronary atherosclerosis have been carried out. The causal relationship has not yet been proven, but ongoing large intervention trials and research on pathogenetic mechanisms may lead to the use of antimicrobial agents in the treatment of CHD in the future.

  5. Mycoplasma pneumoniae meningoencephalitis: a case report

    Directory of Open Access Journals (Sweden)

    Mehmet Selçuk Bektaş

    2013-01-01

    Full Text Available Nervous system is the most affected area in mycoplasma pneumoniae infections with exception of respiratory system. It is an important agent of childhood acute encephalitis and respiratory system infections in school-age children and young adults. Routine clinical and laboratory findings to identify spesific diagnosis is limited. Twelve-year-old female patient was admitted with fever, fatigue, sore throat, slipping the right eye, withdrawal of the mouth from the right and right hemiclonic seizures. Test of anti-Mycoplasma pneumoniae (M. pneumoniae IgM was positive and IgG antibodies were found to be 4-fold increase in the sera of follow-up. This article was presented with the aim of remembering M. pneumoniae to be an differential diagnosis in children with acute encephalitis.

  6. Comparison of polymerase chain reaction tests for diagnosis of feline herpesvirus, Chlamydophila felis, and Mycoplasma spp. infection in cats with ocular disease in Canada.

    Science.gov (United States)

    Sandmeyer, Lynne S; Waldner, Cheryl L; Bauer, Bianca S; Wen, Xin; Bienzle, Dorothee

    2010-06-01

    This study assessed the value of polymerase chain reaction (PCR) for making a diagnosis of feline herpesvirus (FHV-1) infection, and for differentiating this from Chlamydophila felis and Mycoplasma spp. infection in a clinical setting in Canada. We compared the frequency of positive FHV-1 PCR test results from 48 clinical cases of ocular disease in cats suspected to be due to FHV-1 between 1 research and 2 commercial laboratories in Canada. We also compared PCR results for Chlamydophila felis and Mycoplasma spp. between the 2 commercial laboratories. The prevalence of FHV-1 infection in the cats ranged from 4% to 21%. The prevalence of Chlamydophila felis was 2% and 17% and the prevalence of Mycoplasma spp. was 11% and 27%. Agreement between FHV-1 culture and PCR results at the research laboratory was substantial (kappa = 0.76). There was slight agreement (kappa Chlamydophila felis (kappa = 0.2) and Mycoplasma spp. (kappa = 0.07) PCR.

  7. Chlamydophila pneumoniae induces a sustained airway hyperresponsiveness and inflammation in mice

    NARCIS (Netherlands)

    F. Blasi (Francesco); S. Aliberti (Stefano); L. Allegra (Luigi); G. Piatti (Gioia); P. Tarsia (Paolo); J.M. Ossewaarde (Jacobus); V. Verweij (Vivienne); F.P. Nijkamp (Frans); G. Folkerts (Gert)

    2007-01-01

    textabstractBackground: It has been reported that Chlamydophila (C.) pneumoniae is involved in the initiation and promotion of asthma and chronic obstructive pulmonary diseases (COPD). Surprisingly, the effect of C. pneumoniae on airway function has never been investigated.Methods: In this study, mi

  8. Localizzazione e valutazione dell’espressione di Chlamydophila pneumoniae mediante RT-PCR in situ

    Directory of Open Access Journals (Sweden)

    Stefania Cazzavillan

    2005-12-01

    Full Text Available Chlamydophila pneumoniae, an obligate intracellular gram negative bacterium, is involved in a wide spectrum of symptomatic respiratory tract diseases. However more recently it has been reported to be a pathogenic agent in the mechanism leading to atherosclerosis. In the present study the presence of Chlamydophila pneumoniae was assessed, using nested PCR and in situ PCR, while the viability of the microorganism was investigated using RT in situ PCR.The results obtained demonstrated that Chlamydophila pneumoniae was present and alive in the tissues examined.The global concordance of results in the three techniques used was 100%. RT in situ PCR can be considered a precious tool to detect bacterial mRNA in formalin fixed paraffin embedded samples provided an optimal standardization of the key variables is achieved.

  9. The clinical characteristics,treatment and outcome of macrolide-resistant Mycoplasma pneumoniae pneumonia in children

    Institute of Scientific and Technical Information of China (English)

    鲍芳

    2013-01-01

    Objective To investigate the drug resistance of My-coplasma pneumoniae among children with community-acquired pneumonia (CAP) ,and to explore the clinical and radiological characteristics of and the role of azithromycin in the treatment of of macrolide-resistant (MR) Mycoplasma pneumoniae pneumonia.Methods Cases of CAP in children (n=179) were prospectively enrolled in

  10. CNS Complications of Mycoplasma Pneumoniae

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2004-08-01

    Full Text Available Three cases of acute central nervous system disease occurring subsequent to infection with M pneumoniae are reported from University College, Institute of Child Health, and Great Ormond Street Hospital, London, UK.

  11. Clinical features of severe or fatal Mycoplasma pneumoniae pneumonia

    Directory of Open Access Journals (Sweden)

    Koichi eIzumikawa

    2016-06-01

    Full Text Available Mycoplasma pneumoniae is one of the most common causes of community-acquired pneumonia in children and young adults. The incidence of fulminant M. pneumoniae pneumonia (MPP is relatively rare despite the high prevalence of M. pneumoniae infection. This literature review highlights the clinical features of fulminant MPP by examining the most recent data in epidemiology, clinical presentation, pathogenesis, and treatment. Fulminant MPP accounts for 0.5-2% of all MPP cases and primarily affects young adults with no underlying disease. Key clinical findings include a cough, fever, and dyspnea along with diffuse abnormal findings in radiological examinations. Levels of inflammatory markers such as white blood cells (WBC and C-reactive protein (CRP are elevated, as well as levels of lactate dehydrogenase (LDH, IL-18, AST, and ALT. The exact pathogenesis of fulminant MPP remains unclear, but theories include a delayed hypersensitivity reaction to Mycoplasma pneumoniae and the contribution of delayed antibiotic administration to disease progression. Treatment options involve pairing the appropriate anti-mycoplasmal agent with a corticosteroid that will downregulate the hypersensitivity response, and mortality rates are quite low in this treatment group. Further research is necessary to determine the exact pathogenesis of severe and fulminant types of MPP.

  12. Mycoplasma pneumoniae meningoencephalitis:a case report

    Institute of Scientific and Technical Information of China (English)

    Mehmet Selçuk Bektaş; Fesih Aktar; Mehmet Açıkgöz; Ertan Sal; HüseyinÇaksen

    2013-01-01

    Nervous system is the most affected area inmycoplasma pneumoniaeinfections with exception of respiratory system.It is an important agent of childhood acute encephalitis and respiratory system infections in school-age children and young adults.Routine clinical and laboratory findings to identify spesific diagnosis is limited.Twelve-year-old female patient was admitted with fever, fatigue, sore throat, slipping the right eye, withdrawal of the mouth from the right and right hemiclonic seizures.Test of anti-Mycoplasma pneumoniae(M. pneumoniae)IgM was positive andIgG antibodies were found to be4-fold increase in the sera of follow-up.This article was presented with the aim of rememberingM. pneumoniae to be an differential diagnosis in children with acute encephalitis.

  13. Increased infiltration of Chlamydophila pneumoniae in the vessel wall of human veins after perfusion

    NARCIS (Netherlands)

    Kupreishvili, K.; Weeme, M.ter; Morré, S.A.; Brule, A.J.C. van den; Huybregts, M.A.J.M.; Quax, P.H.A.; Velden, J. ten; Hinsbergh, V.W.M. van; Stooker, W.; Eijsman, L.; Niessen, H.W.M.

    2008-01-01

    Background: Several studies have suggested an association between Chlamydophila pneumoniae (Cp) infection and atherosclerosis. A recent study detected Cp DNA in the saphenous vein of 12% of all patients before bypass grafting and in 38% of failed grafts. We used a system in which human veins were pe

  14. Seropositivity for Chlamydia Pneumoniae and Mycoplasma Pneumoniae in Asthmatic Children

    Directory of Open Access Journals (Sweden)

    Murat Tutanc

    2014-03-01

    Full Text Available Acute respiratory tract infections may trigger acute asthma attacks and may be held responsible for etiopathogenesis in children with asthma. Although bacterial infections attract a limited amount of attention, recently Chlamydia pneumoniae (CP and Mycoplasma pneumoniae (MP, in particular, are reported to be the possible factors. IgM and IgG seroprevalence was investigated in 66 children patients with bronchial asthma (between the ages of 3 and 14 for CP and Mycoplasma pneumoniae. In a total of 66 cases, 18 (27.2% patients were detected with IgG positivity for CP whereas 27 of them (40.9% were detected with IgG positivity for MP. IgG positivity was determined in 6 patients (13.0% in the control group for CP, and in 6 patients (10.8% in the control group for MP. The rate of the asthma patients with IgG seropositivity for MP was 4 times higher than that of the control group. It was seen that IgG antibody seropositivity for CP was higher in those with more frequent attacks. No such difference was observed in terms of IgG antibody seropositivity for M. pneumoniae. There are many studies indicating that CP and MP infections take an importance place in the etiology of bronchial asthma and asthma attacks in children. The results obtained reveal the effect of both microorganisms on the etiopathogenesis of the bronchial asthma and the increased number of asthma attacks.

  15. Association of Mycoplasma pneumoniae infection and childhood asthma

    Institute of Scientific and Technical Information of China (English)

    YADAV Shakti Nrisingh; GAUTAM Mahesh Kumar; JIANG Li

    2015-01-01

    Mycoplasma pneumoniae is a frequent cause of acute respiratory infections in both children and adults.It can cause pharyngitis, otitis, tracheobronchitis, or community-acquired pneumonia, but may also remain totally asymptomatic.Mycoplasma pneumoniae is an organism that reportedly has a strong relationship to asthma.The role of atypical bacterial infection in the pathogenesis of asthma is a subject of continuing debate. There is an increasing body of literature concerning the association between Mycoplasma pneumoniae ( M. pneumoniae) and asthma pathogenesis.Moreover, many studies investigating such a link have been uncontrolled and have provided conflicting evidence, in part due to the difficulty in accurately diagnosing infection with these atypical pathogens. Large, general population-based prospective studies are necessary to investigate the development of asthma induced by M. pneumoniae infection in humans. This manuscript will review the relationship between M.pneumoniae infection and childhood asthma.

  16. Meningoencefalitis por Mycoplasma pneumoniae en un adulto joven Mycoplasma pneumoniae meningoencephalitis in a young adult

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    Marcelo del Castillo

    2005-08-01

    Full Text Available Las infecciones por Mycoplasma pneumoniae presentan complicaciones del sistema nervioso de distinto tipo, que si bien son infrecuentes pueden ser graves; habitualmente tienen evolución favorable pero pueden dejar secuelas permanentes. Se presenta una paciente adulta joven con una meningoencefalitis aguda que no dejó secuelas como complicación de una infección respiratoria baja. El diagnóstico se efectuó por detección de anticuerpos específicos.Mycoplasma pneumoniae infections have extrapulmonary complications that involve the nervous system. The neurologic manifestations are diverse. Although the prognosis is usually favorable, the patients can undergo severe permanent sequelae. We present a young female adult with acute meningoencephalitis as a complication of a lower respiratory infection, which followed a benign course without neurologic sequelae.

  17. Catalase Enhances Growth and Biofilm Production of Mycoplasma pneumoniae.

    Science.gov (United States)

    Simmons, Warren L; Dybvig, Kevin

    2015-08-01

    Mycoplasma pneumoniae causes chronic respiratory disease in humans. Factors thought to be important for colonization include the ability of the mycoplasma to form a biofilm on epithelial surfaces and the production of hydrogen peroxide to damage host tissue. Almost all of the mycoplasmas, including M. pneumoniae, lack superoxide dismutase and catalase and a balance should exist between peroxide production and growth. We show here that the addition of catalase to cultures enhanced the formation of biofilms and altered the structure. The incorporation of catalase in agar increased the number of colony-forming units detected and hence could improve the clinical diagnosis of mycoplasmal diseases.

  18. Immunogenicity of Cpn0425 and its localization in cells infected with Chlamydophila pneumoniae.

    Science.gov (United States)

    Liu, Liangzhuan; You, Xiaoxing; Chen, Liesong; Zeng, Yanhua; Tang, Shuangyang; Yu, Minjun; Wu, Yimou; Xhen, Xi

    2012-12-01

    The present study aimed to determine the intracellular localization of Cpn0425 in Chlamydophila pneumoniae-infected cells. The recombinant plasmid pGEX-6p/Cpn0425 was transformed into E.coli Bl21 cells to express the fusion protein. Following purification with glutathione S-transferase (GST) resin chromatography, the Cpn0425 fusion protein was used to induce immunity in mice to develop monoclonal and polyclonal antibodies, which were subsequently used to localize the endogenous Cpn0425 protein by indirect immunofluorescence assay (IFA). ELISA was used to determine the immunogenicity of the Cpn0425 plasmid protein by recognizing the pool sera of patients infected with Chlamydia trachomatis and the pool sera of mice immunized with the Cpn0425 fusion protein. The Cpn0425 gene was expressed as the GST-Cpn0425 fusion protein in E. coli and its antibody was prepared by immunizing mice with the fusion protein. An anti-GST-Cpn0425 antibody was used to localize the protein in cells infected with Chlamydophila pneumoniae AR-39 using an IFA. The anti-GST-CT058 antibody detected an inclusion signal in the IFA. Cpn0425 protein strongly reacted with antiserum. Although Cpn0425 protein is not a secreted protein, it has good immunogenicity. Therefore, this protein may be useful for developing vaccines against Chlamydophila pneumoniae infection.

  19. ASSOCIATION OF MYCOPLASMA PNEUMONIAE WITH RESPIRATORY TRACT INFECTIONS IN CHILDREN

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    Osama Mohammed Saed Abdul-Wahab

    2013-01-01

    Full Text Available Mycoplasma pneumoniae is one of four most common species of organisms that are responsible for most clinically significant infections in humans. It is a frequent cause of acute respiratory infections in both children and adults. The organism can cause pharyngitis, otitis, tracheobronchitis, or community-acquired pneumonia, but patients may also remain totally asymptomatic. Aim of this prospective study for children, was to investigate the association of M. pneumoniae with respiratory tract infections in a Saudi population. This study was designed as a case-control study in which 90 patients (Mean age of the patients in case group was 5.94±2.73 and in control group was 6.51±2.26 of either sexes were included. These patients were classified into two groups: first group (case group, included 45 patients who had been admitted in hospital with diagnosis of respiratory tract infections and the second group (control group, included 45 healthy patients who had no history of respiratory tract infections. Both the groups were age and sex matched. Presence of IgM antibodies to Mycoplasma pneumoniae was assessed by ELISA technique in both groups. In the case group, 4 (9% cases out of 45 children were positive for anti-mycoplasma antibody whereas in the control group, all children were negative. All positive case group patients had symptoms of acute pneumonia. 18 (40% of the patients were diagnosed with bronchial asthma (40% inclusive of all the four cases diagnosed with Mycoplasma pneumoniae infection. The relative risk for the occurrence of mycoplasma infection was estimated to be 9 (95%C.I = 0.49-162.43. However, on comparing the case and control groups, the result was not found to be statistically significant. (Fischer Exact Test p = 0.0583. Children in Saudi Arabia are at a relatively higher risk of developing Mycoplasma pneumoniae infection especially those predisposed with underlying chronic respiratory illnesses such as asthma. This is a first

  20. The inhibitory effect of disodium cromoglycate on the growth of Chlamydophila (Chlamydia) pneumoniae in vitro.

    Science.gov (United States)

    Yamazaki, Tsutomu; Yamaguchi, Tetsuya; Sasaki, Nozomu; Inoue, Miyuki; Sato, Kozue; Kishimoto, Toshio

    2006-04-01

    Chlamydophila (Chlamydia) pneumoniae is associated with asthma and several other respiratory illnesses. Disodium cromoglycate (DSCG) is known to inhibit both immediate and late asthmatic responses. In this study, the inhibitory effect of DSCG on the growth of C. pneumoniae was examined by minimum inhibitory concentration (MIC) and pre-inoculation minimal cidal concentration (MCC) assays using HL cells and C. pneumoniae AR-39. DSCG below the clinically relevant concentration inhibited the growth of C. pneumoniae in a dose-dependent manner in both the MCC and MIC assays. The inhibitory effect was also time-dependent in the MCC assay at 20 mg/ml of DSCG. These results warrant further clinical study on the connection between C. pneumoniae infections and use of DSCG.

  1. Mycoplasma pneumoniae associated organising pneumonia in a 10 year old boy.

    Science.gov (United States)

    Wachowski, O; Demirakça, S; Müller, K-M; Scheurlen, W

    2003-03-01

    We describe a 10 year old boy with organising pneumonia associated with acute Mycoplasma pneumoniae infection. The diagnosis of organising pneumonia was made by open lung biopsy and the M pneumoniae infection was proven serologically. Antibiotic and long term corticosteroid treatment resulted in steadily improving pulmonary function monitored by spirometry. The introduction of anti-inflammatory treatment with NSAIDs/immunosuppressive agents in order to spare steroids was well tolerated and resulted in further improvement of the pulmonary function. To our knowledge this is the first documented case of Mycoplasma pneumoniae associated organising pneumonia to be reported in a child.

  2. Association of Mycoplasma pneumoniae Infection with Myocardial Infarction

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    Abdolreza Sotoodeh Jahromy

    2009-01-01

    Full Text Available Problem statement: In addition to the major risk factors for atherosclerosis such as high plasma level of low density lipoprotein, low plasma level of high density lipoprotein, cigarette smoking, hypertension and diabetes mellitus, some studies introduce other agents such as Mycoplasma pneumoniae as risk factors for atherodclerosis and coronary artery diseases. Aim of this study was to clarify the risk of Mycoplasma pneumoniae for myocardial infarction in Iranian population. Approach: This was a case-control study, in which 90 patients studied. (March 2005-2007. First group (or case group include 45 units who had been admitted in hospital with diagnosis of myocardial infarction and second group include 45 units, who were healthy individuals without any positive history of ischemic heart disease. IgG antibodies was assessed by ELISA technique in both groups. Results: There was significant statistic difference in antimycoplasma antibody level. In the groups (p = 0.028 and the relative risk of mycoplasma infection for myocardial infarction estimated to be 2.7. Conclusion: Mycoplasma pneumoniae infection seems to be a risk factor for myocardial infarctin, in Iranian population. It is better to design other studies to evaluate the risk of coinfection of Mycoplasma pneumoniae and Chlamydia pneumoniae and also the risk of these infection plus conventional risk factors for myocardial infarction in this country.

  3. Genomic screening for Chlamydophila pneumoniae-specific antigens using serum samples from patients with primary infection.

    Science.gov (United States)

    Yasui, Yumiko; Yanatori, Izumi; Kawai, Yasuhiro; Miura, Koshiro; Suminami, Yoshinori; Hirota, Tomomitsu; Tamari, Mayumi; Ouchi, Kazunobu; Kishi, Fumio

    2012-04-01

    Chlamydophila pneumoniae, an obligate intracellular human pathogen, causes respiratory tract infections. The most common techniques used for the serological diagnosis of C. pneumoniae infections are microimmunofluorescence tests and commercial serological ELISA tests; these are based on the detection of antibodies against whole chlamydial elementary bodies and lipopolysaccharide/outer membrane protein, respectively. Identification of more specific and highly immunodominant antigens is essential for the development of new serodiagnostic assays. To identify novel specific antigens from C. pneumoniae, we screened 455 genes with unknown function in the genome of C. pneumoniae J138. Extracts of Saccharomyces cerevisiae cells expressing GFP-tagged C. pneumoniae proteins were subjected to Western blot analysis using serum samples from C. pneumoniae-infected patients as the primary antibodies. From this comprehensive analysis, 58 clones expressing C. pneumoniae open reading frames, including hypothetical proteins, were identified as antigens. These results have provided useful information for the development of new serological tools for the diagnosis for C. pneumoniae infections and for the development of vaccines in future.

  4. Early Mycoplasma pneumoniae infection presenting as multiple pulmonary masses: an unusual presentation in a child

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Edward; Altes, Talissa; Anupindi, Sudha A. [The Children' s Hospital of Philadelphia, Department of Radiology, Philadelphia, PA (United States)

    2008-04-15

    Mycoplasma pneumoniae is a major cause of community-acquired pneumonia. Because most children are not imaged prior to onset of clinical symptoms, the appearance of early Mycoplasma infection has not been extensively studied. We present the case of an 11-year-old boy with large pulmonary masses incidentally detected during spine MRI evaluation for scoliosis. Eight days later, the patient developed acute respiratory symptoms, and the masses seen previously had evolved into a diffuse bronchiolitis. Diagnostic testing identified Mycoplasma pneumoniae as the likely etiology. We briefly review chest CT findings of infection by Mycoplasma and compare them to this unusual presentation of Mycoplasma pneumonia with subclinical imaging findings. (orig.)

  5. Severe community-acquired pneumonia caused by Mycoplasma pneumoniae in young female patient

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    Milačić Nena

    2015-07-01

    Full Text Available Mycoplasma pneumonia is common agent causing community acquired pneumonia in younger population. However, the course of illness is usually benign and is rarely associated with pulmonary complications. We report a 27 years old female patient with unilateral pneumonia followed by pleural effusion and adhesions on the same side. This potential source of infection should be considered in young patients where resolution of symptoms from pneumonia is delayed.

  6. Epidemic of Mycoplasma pneumoniae infection in Denmark, 2010 and 2011

    DEFF Research Database (Denmark)

    Uldum, S A; Bangsborg, J M; Gahrn-Hansen, B;

    2012-01-01

    Denmark experienced two waves of Mycoplasma pneumoniae infection during autumn and early winter in 2010 and 2011, respectively. Both affected the whole country. The proportion of positive results was almost the same for both, indicating that the two waves were probably of equal size. High macroli...... consumption during the epidemics did not seem to affect levels of macrolide resistance in M. pneumoniae, which remain low in Demark (1% to 3%)....

  7. Multiple organ dysfunction syndrome associated with Mycoplasma pneumoniae infection

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    Shu-Bo Zhai

    2012-03-01

    Full Text Available In this study, we report one case of a three-year-old boy infected with Mycoplasma pneumonia (MP and presenting concomitant multiple organ damage of the heart, kidney, lung and liver, among others, together with a brief review for the diagnosis and treatment of MP infection with multiple organ dysfunction syndrome (MODS.

  8. Zoonotic atypical pneumonia due to Chlamydophila psittaci: first reported psittacosis case in Taiwan.

    Science.gov (United States)

    Cheng, Yu-Jen; Lin, Kun-Yen; Chen, Chun-Chen; Huang, Yen-Lin; Liu, Chun-Eng; Li, Shu-Ying

    2013-07-01

    Human psittacosis caused by Chlamydophila psittaci is one of the most common zoonotic atypical pneumonias featuring pulmonary as well as extrapulmonary infections. Most of the cases involve avian contact history especially with psittacine birds. Herein we report a 44-year-old male patient displaying atypical pneumonia symptoms of intermittent fever, dry cough, chest pain, dyspnea, headache, hepatitis, and hyponatremia. He had two sick cockatiels, one of which had died a month previously. A microimmunofluorescence test was performed to check the serum antibody levels against Chlamydophila psittaci. The serum IgM titer showed positive titer of 1:256, 1:256, and 1:128 on Days 11, 23, and 43 after disease onset, respectively. His fever subsided soon and clinical symptoms improved after minocycline was administrated on Day 12. The psittacosis case was confirmed by history of psittacine bird contact, clinical symptoms, treatment response, and positive IgM titer. To our knowledge, this is the first report of a psittacosis case in Taiwan.

  9. Inhibition of apoptosis in neuronal cells infected with Chlamydophila (Chlamydia pneumoniae

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    Albert Elizabeth V

    2008-01-01

    Full Text Available Abstract Background Chlamydophila (Chlamydia pneumoniae is an intracellular bacterium that has been identified within cells in areas of neuropathology found in Alzheimer disease (AD, including endothelia, glia, and neurons. Depending on the cell type of the host, infection by C. pneumoniae has been shown to influence apoptotic pathways in both pro- and anti-apoptotic fashions. We have hypothesized that persistent chlamydial infection of neurons may be an important mediator of the characteristic neuropathology observed in AD brains. Chronic and/or persistent infection of neuronal cells with C. pneumoniae in the AD brain may affect apoptosis in cells containing chlamydial inclusions. Results SK-N-MC neuroblastoma cells were infected with the respiratory strain of C. pneumoniae, AR39 at an MOI of 1. Following infection, the cells were either untreated or treated with staurosporine and then examined for apoptosis by labeling for nuclear fragmentation, caspase activity, and membrane inversion as indicated by annexin V staining. C. pneumoniae infection was maintained through 10 days post-infection. At 3 and 10 days post-infection, the infected cell cultures appeared to inhibit or were resistant to the apoptotic process when induced by staurosporine. This inhibition was demonstrated quantitatively by nuclear profile counts and caspase 3/7 activity measurements. Conclusion These data suggest that C. pneumoniae can sustain a chronic infection in neuronal cells by interfering with apoptosis, which may contribute to chronic inflammation in the AD brain.

  10. Is there any relationship between Chlamydophila pneumoniae and coronary atherosclerosis among Iranians?

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    Mohammad Hadi Sadeghian

    2013-01-01

    Full Text Available Background: Atherosclerosis is a coronary heart disease, andis the most common cause of death in the industrialized world. Some studies suggested that atherosclerosis may be triggered by infectious agents, mostly Chlamydophila pneumoniae. However, the role of C. pneumoniae in the pathogenesis of coronary atherosclerosis is still controversial. Objectives: This study was performed to evaluate whether there is a significant association between coronary artery atherosclerosis and C. pneumoniae by the polymerase chain reaction (PCR method. Materials and Methods: This case-control study was carried out on formalin-fixed paraffin-embedded tissue biopsies of the coronary arteries obtained from 30 patients with coronary atherosclerosis and 30 subjects without atherosclerosis living in Northeast of Iran. All subjects′ weight and height were determined, and the body mass index was calculated. We also reviewed the medical history and previous laboratory reports of patients. Deoxyribonucleic acid (DNA was extracted, and C. pneumonia DNA was amplified and detected using PCR assay. Results: The age of the patients in the study group was from 18 to 50 years, and the male to female ratio was 5:1. Only oneout of the 30 coronary tissue samples had positive PCR for C. pneumoniae (3.3%, while it was negative for patients in the control group. Conclusions: This study showed that C. pneumoniae infection is not strongly associated with coronary artery atherosclerosis in Northeast of Iran.

  11. Seropositivity ofChlamydophila pneumoniae immunoglobulin G antibody of HIV/AIDS patients in Abuja, Nigeria

    Institute of Scientific and Technical Information of China (English)

    Yakubu Boyi Ngwai; Izebe KS; Ijele IG; Ishaleku D; Inyang US

    2010-01-01

    Objective:To detect IgG antibody toChlamydophila pneumoniae(CP)in sera ofHIV/AIDS patients and provide rationale for inclusion of routine screening for anti-CP antibodies and anti-chlamydial agents in the Nigerian NationalHIV/AIDS Management Plan.Methods: Serum samples from34 consentingHIV/AIDS patients attended a Government-approved Antiretroviral Treatment Facility in Abuja were screened by enzyme-linked immunosorbent assay for anti-CP IgG antibody using ImmunoComb® Chlamydia Bivalent IgG Test kit (Orgenics, Israel).Results:Anti-CP IgG antibody was detected in20 (58.8%)of34 patients tested. The detection rate was higher among the males(8/13; 61.5%) than the females (12/21; 57.1%). Patients of the age group 16-30 years had the highest(7/10; 70%) detection of anti-CP IgG antibody.Conclusions:The result of the present study suggests the presence of anti-CP antibodies in sera of the HIV/AIDS patients, and reinforces the need for routine screening for anti-CPantibodies as a necessary intervention to reduce the burden ofChlamydophila pneumoniae (C. pneumoniae) infections and to reduceHIV-positive morbidity in Nigeria. The outcome of this study also provides justification for the possible inclusion of anti-chlamydial agents in the NationalHIV/AIDS Management Plan to provide prophylaxis against or treat activeC. pneumoniae infections.

  12. Pneumomediastinum and pneumothorax as presenting signs in severe Mycoplasma pneumoniae pneumonia

    Energy Technology Data Exchange (ETDEWEB)

    Vazquez, Jose L.; Vazquez, Ignacio; Garcia-Tejedor, Jose L. [Complejo Hospitalario Universitario de Vigo, Department of Radiology, Vigo (Spain); Gonzalez, Maria L.; Reparaz, Alfredo [Complejo Hospitalario Universitario de Vigo, Department of Pediatrics, Vigo (Spain)

    2007-12-15

    We present a 3-year-old child with severe extensive Mycoplasma pneumoniae pneumonia complicated with pneumomediastinum and pneumothorax. Pneumothorax and pneumomediastinum have only exceptionally been described in mild cases of the disease. The radiological findings, differential diagnosis and clinical course are discussed. (orig.)

  13. Elevata espressione di hsp-60 di Chlamydophila pneumoniae su placche aterosclerotiche carotidee a prognosi infausta

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    Rosario Cultrera

    2007-06-01

    Full Text Available Some difficult microorganisms, including Chlamydophila pneumoniae, are associated with the atherosclerotic tissue damage.The aim of this study was to evaluate the employment of culture together molecular techniques in order to define the possible role of C. pneumoniae in the atherosclerotic tissue damage. Atheromatous carotid plaques (ACP were obtained by endoarterectomies from 10 patients with severe stenosis of the internal carotid artery. Each specimen was divided in three parts: a proximal tract to heart, without stenosis, a medial tract, corresponding to the atheromatous plaque, and a distal tract, above the plaque.Aliquots were employed to perform cultures for C. pneumoniae on Hep - 2 cell line in DMEM. DNA and total RNA were extracted from 50-70 mg. of tissue sample and from Hep - 2 106 cultures to investigate 16S rRNA, momp and hsp60 genes.The PCR and RT-PCR resulted negative for momp gene of C. pneumoniae in all samples. PCR and RT-PCR resulted positive for 16S rRNA or hsp60 genes of C. pneumoniae in the proximal portion of two ACPs with hemorrhagic evolution in two patients, one of which complicated with a retinal tromboembolic outcome. Molecular analyses on C. pneumoniae growing from the cultures are in progress.The DNA and RNA amplification of different portions from ACP seems to be useful to evidence the effective localization of C. pneumoniae in the atheromatous arterial tissue. The highly gene expression of C. pneumoniae hsp60 in a patient with acute hemorrhagic evolution of the carotid plaque may suggest that C. pneumoniae might partecipate in the atherogenesis and induce atherosclerosis complications by inflammatory pathways (activation of cytokines, endothelial factors and matrix-degrading metalloproteinases.

  14. Isolamento colturale e molecolare di Chlamydophila pneumoniae da pazienti con artropatie. Prospettive patogenetiche e diagnostiche

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    Margherita Giuliodori

    2007-06-01

    Full Text Available Chlamydophila pneumoniae is an ubiquitous intracellular pathogen which causes acute respiratory diseases and may be associated with chronic inflammatory diseases including atherosclerosis, multiple sclerosis and arthritis. C. pneumoniae is rarely cultured from the synovial fluid or blood, and serology is seldom useful. So far most of the studies concerning the possible association between C. pneumoniae and arthritis have been made by molecular methods. Recent advances in the standardization of polymerase chain reaction techniques have shown to confirm a role of C. pneumoniae not only in reactive arthritis (ReA but also in chronic arthritis. In this study, we investigated whether C. pneumoniae could be isolated in synovial fluid and PBMC specimens of patients with different forms of arthritis including ReA.Advanced PCR and Reverse transcriptase PCR techniques targeting different chlamydial genes associated to a novel culture method based on combination of additional centrifugation and extension of culture time, were applied to detect C. pneumoniae in 6 patients with chronic synovitis including one with Anchylosing Spondylitis and relapsing joint swelling. For this patient, serological, coltural as well as molecular assays did detect C. pneumoniae only. Particularly, a high expression of Heat shock protein 60 and 70 of C. pneumoniae was found in the PBMC and the synovial compartments, thus confirming the ability of C. pneumoniae to survive inside blood ad synovia in vital and metabolically active forms. By contrast, the selective decrease of MOMP and 16sRNA, leads to hypotesize a different expression of Chlamydophyla genes during the different phases of infection.

  15. Chlamydophila pneumoniae induces a sustained airway hyperresponsiveness and inflammation in mice

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    Verweij Vivienne

    2007-11-01

    Full Text Available Abstract Background It has been reported that Chlamydophila (C. pneumoniae is involved in the initiation and promotion of asthma and chronic obstructive pulmonary diseases (COPD. Surprisingly, the effect of C. pneumoniae on airway function has never been investigated. Methods In this study, mice were inoculated intranasally with C. pneumoniae (strain AR39 on day 0 and experiments were performed on day 2, 7, 14 and 21. Results We found that from day 7, C. pneumoniae infection causes both a sustained airway hyperresponsiveness and an inflammation. Interferon-γ (IFN-γ and macrophage inflammatory chemokine-2 (MIP-2 levels in bronchoalveolar lavage (BAL-fluid were increased on all experimental days with exception of day 7 where MIP-2 concentrations dropped to control levels. In contrast, tumor necrosis factor-α (TNF-α levels were only increased on day 7. From day 7 to 21 epithelial damage and secretory cell hypertrophy was observed. It is suggested that, the inflammatory cells/mediators, the epithelial damage and secretory cell hypertrophy contribute to initiation of airway hyperresponsiveness. Conclusion Our study demonstrates for the first time that C. pneumoniae infection can modify bronchial responsiveness. This has clinical implications, since additional changes in airway responsiveness and inflammation-status induced by this bacterium may worsen and/or provoke breathlessness in asthma and COPD.

  16. Seroprevalence of Chlamydophila pneumoniae in HIV-infected children in Vietnam.

    Science.gov (United States)

    Trinh, Quang Duy; Pham, Ngan Thi Kim; Le Nguyen, Nhut Tin; Lam, Bao Quoc; Le Phan, Kim Thoa; Truong, Khanh Huu; Le, Thinh Quoc; Nguyen, Hung Thanh; Tang, Thuong Chi; Izumi, Yasuyuki; Komine-Aizawa, Shihoko; Ushijima, Hiroshi; Hayakawa, Satoshi

    2013-06-01

    A total of 89 blood samples collected from HIV-infected infants and children from provinces of southern Vietnam who were hospitalized at Children's Hospital 1, Ho Chi Minh City, during the 1-year period from October 2004 to September 2005 were submitted to serological screening for IgG, IgA, and IgM antibodies against Chlamydophila pneumoniae (C. pneumoniae). The presence of this microorganism was also evaluated by PCR. The results showed that 64 % of the samples were positive for anti-C. pneumoniae IgG, 31.5 % were positive for IgA, and 3.4 % were positive for IgM. The highest prevalences of IgG and IgA positivity, 75 % and 66.7 %, respectively, were noted in the 1- to 2-year-old age group. However, all the samples were negative for C. pneumoniae by PCR. The study revealed a high seroprevalence of C. pneumoniae in Vietnamese infants and children with HIV/AIDS.

  17. Infection by Mycoplasma pneumoniae and its importance as an etiological agent in childhood community-acquired pneumonias

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    Letícia Alves Vervloet

    2007-10-01

    Full Text Available This manuscript reviewed the literature on infection by Mycoplasma pneumoniae with emphasis on etiological aspects of childhood community-acquired pneumonias. Bibliographical research was carried out from Pubmed Medline, MDConsult, HighWire, LILACS, and direct research over the past 10 years with the following keywords: Mycoplasma pneumoniae, pneumonia, and childhood. Fifty-four articles were selected. Mycoplasma pneumoniae has a high incidence in childhood. Clinical presentation includes respiratory and extrarespiratory symptoms. Mycoplasma pneumoniae lung infection can be confused with viral or bacterial pneumonia and is unresponsive to beta-lactams. In addition, co-infections have been reported. Mycoplasma pneumoniae infection occurs in all age groups, being less frequent and more severe in children under the age of five. Its incidence as a causal agent is high. Mycoplasma pneumoniae infections constitute 20%-40% of all community-acquired pneumonias; the severity is highly variable, and this condition may lead to severe sequelae. Mycoplasma pneumoniae frequency is underestimated in clinical practice because of the lack of specific features and a diagnosis that needs serology or PCR. Effective management of M. pneumoniae infections can usually be achieved with macrolides. In Brazil, epidemiological studies are needed in order to assess the incidence of this bacterium.

  18. Cortical Blindness in a Child Secondary to Mycoplasma pneumoniae Infection.

    Science.gov (United States)

    Garcia Tirado, A; Jimenez-Rolando, B; Noval, S; Martinez Bermejo, A

    2017-01-01

    Our objective is to present a case of an uncommon complication associated with Mycoplasma pneumoniae infection in a child where cortical blindness was the main clinical feature. Stroke due to an infection by M. pneumoniae is very uncommon. No consensus has been reached on the pathogenesis, although several pathogenic mechanisms have been proposed. Occlusion of posterior cerebral circulation is the most uncommon central nervous system complication of M. pneumoniae infection being reported. Symptoms are usually hemiplegia and dysarthria. We report a case of a 6-year-old boy who suffered cortical blindness due to a stroke 2 days after M. pneumoniae infection. This is the first case of documented cortical blindness due to posterior cerebral arteries occlusion in children after M. pneumoniae infection.

  19. Differences in Cell Activation by Chlamydophila pneumoniae and Chlamydia trachomatis Infection in Human Endothelial Cells

    Science.gov (United States)

    Krüll, M.; Kramp, J.; Petrov, T.; Klucken, A. C.; Hocke, A. C.; Walter, C.; Schmeck, B.; Seybold, J.; Maass, M.; Ludwig, S.; Kuipers, Jens G.; Suttorp, N.; Hippenstiel, S.

    2004-01-01

    Seroepidemiological studies and demonstration of viable bacteria in atherosclerotic plaques have linked Chlamydophila pneumoniae infection to the development of chronic vascular lesions and coronary heart disease. In this study, we characterized C. pneumoniae-mediated effects on human endothelial cells and demonstrated enhanced phosphorylation and activation of the endothelial mitogen-activated protein kinase (MAPK) family members extracellular receptor kinase (ERK1/2), p38-MAPK, and c-Jun-NH2 kinase (JNK). Subsequent interleukin-8 (IL-8) expression was dependent on p38-MAPK and ERK1/2 activation as demonstrated by preincubation of endothelial cells with specific inhibitors for the p38-MAPK (SB202190) or ERK (U0126) pathway. Inhibition of either MAPK had almost no effect on intercellular cell adhesion molecule 1 (ICAM-1) expression. While Chlamydia trachomatis was also able to infect endothelial cells, it did not induce the expression of endothelial IL-8 or ICAM-1. These effects were specific for a direct stimulation with viable C. pneumoniae and independent of paracrine release of endothelial cell-derived mediators like platelet-activating factor, NO, prostaglandins, or leukotrienes. Thus, C. pneumoniae triggers an early signal transduction cascade in target cells that could lead to endothelial cell activation, inflammation, and thrombosis, which in turn may result in or promote atherosclerosis. PMID:15501794

  20. The NOD/RIP2 pathway is essential for host defenses against Chlamydophila pneumoniae lung infection.

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    Kenichi Shimada

    2009-04-01

    Full Text Available Here we investigated the role of the Nod/Rip2 pathway in host responses to Chlamydophila pneumoniae-induced pneumonia in mice. Rip2(-/- mice infected with C. pneumoniae exhibited impaired iNOS expression and NO production, and delayed neutrophil recruitment to the lungs. Levels of IL-6 and IFN-gamma levels as well as KC and MIP-2 levels in bronchoalveolar lavage fluid (BALF were significantly decreased in Rip2(-/- mice compared to wild-type (WT mice at day 3. Rip2(-/- mice showed significant delay in bacterial clearance from the lungs and developed more severe and chronic lung inflammation that continued even on day 35 and led to increased mortality, whereas WT mice cleared the bacterial load, recovered from acute pneumonia, and survived. Both Nod1(-/- and Nod2(-/- mice also showed delayed bacterial clearance, suggesting that C. pneumoniae is recognized by both of these intracellular receptors. Bone marrow chimera experiments demonstrated that Rip2 in BM-derived cells rather than non-hematopoietic stromal cells played a key role in host responses in the lungs and clearance of C. pneumoniae. Furthermore, adoptive transfer of WT macrophages intratracheally was able to rescue the bacterial clearance defect in Rip2(-/- mice. These results demonstrate that in addition to the TLR/MyD88 pathway, the Nod/Rip2 signaling pathway also plays a significant role in intracellular recognition, innate immune host responses, and ultimately has a decisive impact on clearance of C. pneumoniae from the lungs and survival of the infectious challenge.

  1. Plasmacytoid dendritic cells prevent cigarette smoke and Chlamydophila pneumoniae-induced Th2 inflammatory responses.

    Science.gov (United States)

    Sorrentino, Rosalinda; Gray, Pearl; Chen, Shuang; Shimada, Kenichi; Crother, Timothy R; Arditi, Moshe

    2010-10-01

    Smoking promotes the development of allergic asthma and pneumonia. Chlamydophila pneumoniae lung infection is associated with an increased risk for asthma, inducing an immune response regulated by dendritic cells (DCs). This study sought to determine whether exposure to cigarette smoke modulates the functional activity of CD11c-positive DCs in the lung, with and without concomitant C. pneumoniae infection. Bone marrow-derived DCs (BMDCs) were exposed in vitro to cigarette smoke extract (CSE) and/or live C. pneumoniae (Cpn), and then adoptively transferred intratracheally into wild-type mice. Although CSE plus Cpn appeared to exert an additive effect on the production of Th2 cytokines in vitro, we did not see this effect in vivo. However, the adoptive transfer of DCs pulsed with both CSE and C. pneumoniae into the lungs of naive mice led to an influx of plasmacytoid DCs (pDCs) that suppressed the Th2 skewing ability of the transferred BMDCs. The depletion of pDCs by antibody restored the Th2 skewing ability of the BMDCs. The expression of indoleamine-2,3-dioxygenase in the lung was reduced after the depletion of pDCs, and blocking IFN-α in vitro prevented the ability of pDCs to inhibit the Th2 responses induced by myeloid DCs (mDCs), suggesting their potential involvement in the mechanism of altered polarization. In conclusion, exposure to cigarette smoke skews C. pneumoniae-induced mDCs responses toward a Th2 bias in the lung, which is prevented by pDCs. We propose that pDCs may play a major role in the immunosuppressive lung environment in smokers with C. pneumoniae infection.

  2. The NOD/RIP2 pathway is essential for host defenses against Chlamydophila pneumoniae lung infection.

    Science.gov (United States)

    Shimada, Kenichi; Chen, Shuang; Dempsey, Paul W; Sorrentino, Rosalinda; Alsabeh, Randa; Slepenkin, Anatoly V; Peterson, Ellena; Doherty, Terence M; Underhill, David; Crother, Timothy R; Arditi, Moshe

    2009-04-01

    Here we investigated the role of the Nod/Rip2 pathway in host responses to Chlamydophila pneumoniae-induced pneumonia in mice. Rip2(-/-) mice infected with C. pneumoniae exhibited impaired iNOS expression and NO production, and delayed neutrophil recruitment to the lungs. Levels of IL-6 and IFN-gamma levels as well as KC and MIP-2 levels in bronchoalveolar lavage fluid (BALF) were significantly decreased in Rip2(-/-) mice compared to wild-type (WT) mice at day 3. Rip2(-/-) mice showed significant delay in bacterial clearance from the lungs and developed more severe and chronic lung inflammation that continued even on day 35 and led to increased mortality, whereas WT mice cleared the bacterial load, recovered from acute pneumonia, and survived. Both Nod1(-/-) and Nod2(-/-) mice also showed delayed bacterial clearance, suggesting that C. pneumoniae is recognized by both of these intracellular receptors. Bone marrow chimera experiments demonstrated that Rip2 in BM-derived cells rather than non-hematopoietic stromal cells played a key role in host responses in the lungs and clearance of C. pneumoniae. Furthermore, adoptive transfer of WT macrophages intratracheally was able to rescue the bacterial clearance defect in Rip2(-/-) mice. These results demonstrate that in addition to the TLR/MyD88 pathway, the Nod/Rip2 signaling pathway also plays a significant role in intracellular recognition, innate immune host responses, and ultimately has a decisive impact on clearance of C. pneumoniae from the lungs and survival of the infectious challenge.

  3. Erythema nodosum caused by ascariasis and Chlamydophila pneumoniae pulmonary infection--a case report.

    Science.gov (United States)

    Bergler-Czop, Beata; Lis-Swiety, Anna; Kamińska-Winciorek, Grazyna; Brzezińska-Wcisło, Ligia

    2009-12-01

    Erythema nodosum belongs to a group of relatively common hypodermal inflammations. It occurs mainly among women, particularly young women. The etiology of the disease is not clear. Most frequently, changes appear on the surface of the frontal part of the shins. Initially, red nodules change in color to dark brown and then to yellow and green. There is neither dissolution nor cicatrization of the exanthema. Regression is frequent. We present a case of erythema nodosum caused by Ascaris lumbricoides infection as well as by an early Chlamydophila pneumoniae infection, whose etiology has rarely been described in the literature. We were not able to confirm which factor was responsible for the occurrence of the skin changes as treatment of both infections was effective and all skin changes later disappeared completely. Particular attention should be paid to the fact that precise diagnosis of a patient and the search for etiologic factors, even rare ones, are crucial to obtain good results with treatment of erythema nodosum.

  4. Ubiquinone (Coenzyme Q) Biosynthesis in Chlamydophila pneumoniae AR39: Identification of the ubiD Gene

    Institute of Scientific and Technical Information of China (English)

    Jun LIU; Jian-Hua LIU

    2006-01-01

    Ubiquinone is an essential electron carrier in prokaryotes. Ubiquinone biosynthesis involves at least nine reactions in Escherichia coli. 3-octaprenyl-4-hydroxybenzoate decarboxylase (UbiD) is an important enzyme on the pathway and deletion of the ubiD gene in E. coli gives rise to ubiquinone deficiency in vivo.A protein from Chlamydophila pneumoniae AR39 had significant similarity compared with protein UbiD from E. coli. Based on this information, the protein-encoding gene was used to swap its counterpart in E. coli, and gene expression in resultant strain DYC was confirmed by RT-PCR. Strain DYC grew using succinate as carbon source and rescued ubiquinone content in vivo, while ubiD deletion strain DYD did not.Results suggest that the chlamydial protein exerts the function of UbiD.

  5. High seroprevalence of Mycoplasma pneumoniae IgM in acute Q fever by enzyme-linked immunosorbent assay (ELISA.

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    Chung-Hsu Lai

    Full Text Available Q fever is serologically cross-reactive with other intracellular microorganisms. However, studies of the serological status of Mycoplasma pneumoniae and Chlamydophila pneumoniae during Q fever are rare. We conducted a retrospective serological study of M. pneumoniae and C. pneumoniae by enzyme-linked immunosorbent assay (ELISA, a method widely used in clinical practice, in 102 cases of acute Q fever, 39 cases of scrub typhus, and 14 cases of murine typhus. The seropositive (57.8%, 7.7%, and 0%, p<0.001 and seroconversion rates (50.6%, 8.8%, and 0%, p<0.001 of M. pneumoniae IgM, but not M. pneumoniae IgG and C. pneumoniae IgG/IgM, in acute Q fever were significantly higher than in scrub typhus and murine typhus. Another ELISA kit also revealed a high seropositivity (49.5% and seroconversion rate (33.3% of M. pneumoniae IgM in acute Q fever. The temporal and age distributions of patients with positive M. pneumoniae IgM were not typical of M. pneumoniae pneumonia. Comparing acute Q fever patients who were positive for M. pneumoniae IgM (59 cases with those who were negative (43 cases, the demographic characteristics and underlying diseases were not different. In addition, the clinical manifestations associated with atypical pneumonia, including headache (71.2% vs. 81.4%, p=0.255, sore throat (8.5% vs. 16.3%, p=0.351, cough (35.6% vs. 23.3%, p=0.199, and chest x-ray suggesting pneumonia (19.3% vs. 9.5%, p=0.258, were unchanged between the two groups. Clinicians should be aware of the high seroprevalence of M. pneumoniae IgM in acute Q fever, particularly with ELISA kits, which can lead to misdiagnosis, overestimations of the prevalence of M. pneumoniae pneumonia, and underestimations of the true prevalence of Q fever pneumonia.

  6. Pneumonia Epizootics in Norwegian Muskoxen Caused by Mycoplasma Ovipneumoniae

    DEFF Research Database (Denmark)

    Handeland, Kjell; Tengs, Torstein; Kokotovic, Branko

    muskoxen killed in the period 2004-2013, as well as Mycoplasma examinations from sheep flocks that were present in the muskox area during the summer of 2012. This study identified M. ovipneumoniae as the plausible primary cause of the muskox pneumonia epidemics both in 2006 and 2012 and domestic sheep...... causes of mortality are animals that are killed when the stray from the area and from railroad accidents. During late summer 2006 and 2012, severe outbreaks of pneumonia with die-offs of 25-30% occurred. During the 2012 outbreak, efforts were made to gather high quality microbiological lung samples from...

  7. Mycoplasma pneumoniae-udløst autoimmun hæmolyse

    DEFF Research Database (Denmark)

    Bohr, Anne Lisbeth; Aagaard, Thomas Granum; Birgens, Henrik;

    2015-01-01

    Mycoplasma pneumoniae is naturally resistant to betalactamase antibiotics but is sensitive to macrolides. Occasionally, infections with M. pneumoniae can lead to severe anaemia due to its ability to cause haemolysis when cold agglutination occurs. Increasing bacterial resistance to macrolid...

  8. Role of E-selectin for diagnosing myocardial injury in paediatric patients with mycoplasma pneumoniae pneumonia.

    Science.gov (United States)

    Deng, Ming-Hong; Lin, Chun-Wang; Sun, Yan-Na; Zeng, Xiang-Lin; Wen, Fang

    2017-01-01

    Backgrounds Effects of myocardial injury on E-selectin remain unclear. Thus, we investigated the diagnostic value of E-selectin for myocardial injury in paediatric patients with mycoplasma pneumoniae pneumonia. Methods In this prospective and blinded clinical study, plasma E-selectin, cardiac troponin I, creatine kinase isoenzyme MB, interleukin-6 and tumor necrosis factor alpha concentrations were measured in paediatric patients with mycoplasma pneumoniae pneumonia (MPP group, n = 138). The control group comprised 120 healthy children. The definition of cardiac injury was based on cardiac troponin I or CK-MB (with or possibly without abnormal electrocardiogram evidence). Diagnostic value of E-selectin for myocardial injury was determined by analysing receiver operating characteristic curves. Results Among the 138 mycoplasma pneumoniae pneumonia patients, 40 patients were identified with myocardial injury, while 98 patients were identified without myocardial injury. Plasma E-selectin concentrations were: 40.22 ± 4.80 ng/mL, in patients with myocardial injury; 18.55 ± 2.16 ng/mL, in patients without myocardial injury and 12.39 ± 3.27 ng/mL, in healthy children. For the 40 patients identified with myocardial injury, area under the receiver operating characteristic curve value for plasma E-selectin concentrations was 0.945 (95% CI: 0.899-0.991), and optimal diagnostic cut-off value was 29.93 ng/mL (positive likelihood ratio = 72.5). Conclusion E-selectin was shown to be an effective index for myocardial injury in paediatric patients with mycoplasma pneumoniae pneumonia, and its role in other causes of myocardial injury warrants further investigation.

  9. Radiologic and clinical findings of mycoplasma pneumonia in children

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    Choi, Yong Jin; Oh, Ki Keun [College of Medicine, Yonsei University, Seoul (Korea, Republic of)

    1987-06-15

    Mycoplasma Pneumonia is a cause of primary atypical pneumonia, but it is asymptomatic mostly or may cause of only mild symptoms. School-aged children experienced high attack rate and manifestation if 'unusual pneumonia' are noted. So authors reviewed clinical and radiological features of 110 cases of serologically proven Mycoplasma pneumonia in hospitalized children between November 1984 and January 1987 retrospectively. The results were as follows ; 1. The sex distribution was 57:53 (1.1:1) in male to female ratio and 47% of them were 5 though 8 years old of age with peak incidence between 5 and 6 years old of age. 2. The symptoms were cough, fever, and sore throat in descending order of frequency and mean symptom duration before admission was 8.1 day. The prevalent season was earlier winter. 3. The radiologic findings were air-space consolidation with lobar, segmental distribution in 68%, interstitial infiltration in 12%, bronchopneumonia in 12%, chronic bronchitis pattern in 3.6%, normal in 4.5%, hilar LN enlargement in 37%, pleural effusion in 12%. 4. Radiologic resolution period was usually 10 days around (4-25 days) and after complete recovery, scarring change or calcification was not seen. 5. Extrapulmonary manifestations were uncommon but hepatitis, hematuria, skin rash, gastroenteritis, myocarditis, otitis media occurred. 6. With administration of tetracyclin and erythromycin, clinical and radiologic responses were promptly seen.

  10. Comprehensive methylome characterization of Mycoplasma genitalium and Mycoplasma pneumoniae at single-base resolution.

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    Maria Lluch-Senar

    Full Text Available In the bacterial world, methylation is most commonly associated with restriction-modification systems that provide a defense mechanism against invading foreign genomes. In addition, it is known that methylation plays functionally important roles, including timing of DNA replication, chromosome partitioning, DNA repair, and regulation of gene expression. However, full DNA methylome analyses are scarce due to a lack of a simple methodology for rapid and sensitive detection of common epigenetic marks (ie N(6-methyladenine (6 mA and N(4-methylcytosine (4 mC, in these organisms. Here, we use Single-Molecule Real-Time (SMRT sequencing to determine the methylomes of two related human pathogen species, Mycoplasma genitalium G-37 and Mycoplasma pneumoniae M129, with single-base resolution. Our analysis identified two new methylation motifs not previously described in bacteria: a widespread 6 mA methylation motif common to both bacteria (5'-CTAT-3', as well as a more complex Type I m6A sequence motif in M. pneumoniae (5'-GAN(7TAY-3'/3'-CTN(7ATR-5'. We identify the methyltransferase responsible for the common motif and suggest the one involved in M. pneumoniae only. Analysis of the distribution of methylation sites across the genome of M. pneumoniae suggests a potential role for methylation in regulating the cell cycle, as well as in regulation of gene expression. To our knowledge, this is one of the first direct methylome profiling studies with single-base resolution from a bacterial organism.

  11. Spontaneous Pneumomediastinum as the presenting sign of Mycoplasma pneumoniae infection

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    Ângela Pereira

    2015-12-01

    Full Text Available Introduction: Spontaneous pneumomediastinum (SP results from nontraumatic mediastinal air leakage. It is uncommon in children requiring a high index of suspicion. Case Report: A 17-year-old tall thin boy, light smoker, with no history of trauma or lung disease presented to our emergency room with symptoms dominated by chest pain and mild dyspnea. He reported a violent cough event in the previous hours. Pneumomediastinum was suspected considering the presence of subcutaneous air in the supraclavicular region, and was confirmed by chest radiograph, which showed mediastinal air. Serology study was positive for Mycoplasma pneumoniae and therefore a macrolide was added to symptomatic treatment, with a complete recovery. Discussion/Conclusion: SP is a diagnosis to consider when facing an adolescent with acute chest pain. This condition is possibly underdiagnosed, given its benign course and mild symptoms. Smoking acts as a predisposing factor. SP has only exceptionally been described in Mycoplasma infection.

  12. Rac1 regulates the NLRP3 inflammasome which mediates IL-1beta production in Chlamydophila pneumoniae infected human mononuclear cells.

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    Julia Eitel

    Full Text Available Chlamydophila pneumoniae causes acute respiratory tract infections and has been associated with development of asthma and atherosclerosis. The production of IL-1β, a key mediator of acute and chronic inflammation, is regulated on a transcriptional level and additionally on a posttranslational level by inflammasomes. In the present study we show that C. pneumoniae-infected human mononuclear cells produce IL-1β protein depending on an inflammasome consisting of NLRP3, the adapter protein ASC and caspase-1. We further found that the small GTPase Rac1 is activated in C. pneumoniae-infected cells. Importantly, studies with specific inhibitors as well as siRNA show that Rac1 regulates inflammasome activation in C. pneumoniae-infected cells. In conclusion, C. pneumoniae infection of mononuclear cells stimulates IL-1β production dependent on a NLRP3 inflammasome-mediated processing of proIL-1β which is controlled by Rac1.

  13. Clinical characteristics of children with Mycoplasma pneumoniae infection hospitalized during the Danish 2010-2012 epidemic

    DEFF Research Database (Denmark)

    Sørensen, Cristel M; Schønning, Kristian; Rosenfeldt, Vibeke

    2013-01-01

    Mycoplasma pneumoniae is a common cause of community-acquired pneumonia. Pneumonia may be the most severe manifestation of respiratory M. pneumoniae infection. The most typical symptoms in children are cough and wheezing, which are often accompanied by upper respiratory tract manifestations...

  14. Clinical characteristics of children with Mycoplasma pneumoniae infection hospitalized during the Danish 2010-2012 epidemic

    DEFF Research Database (Denmark)

    Sørensen, Cristel M; Schønning, Kristian; Rosenfeldt, Vibeke

    2013-01-01

    Mycoplasma pneumoniae is a common cause of community-acquired pneumonia. Pneumonia may be the most severe manifestation of respiratory M. pneumoniae infection. The most typical symptoms in children are cough and wheezing, which are often accompanied by upper respiratory tract manifestations mimic...... mimicking viralrespiratory syndromes....

  15. High Prevalence of Mycoplasma pneumoniae and Chlamydia pneumoniae in Children with Acute Respiratory Infections from Lima, Peru

    Science.gov (United States)

    del Valle-Mendoza, Juana; Orellana-Peralta, Fiorella; Marcelo-Rodríguez, Alvaro; Verne, Eduardo; Esquivel-Vizcarra, Mónica; Silva-Caso, Wilmer; Aguilar-Luis, Miguel Angel; Weilg, Pablo; Casabona-Oré, Verónica; Ugarte, Claudia; del Valle, Luis J.

    2017-01-01

    Background Mycoplasma pneumoniae and Chlamydia pneumoniae are atypical pathogens responsible for pneumonia and a leading cause of morbidity and mortality in low income countries. The study objective is to determine the prevalence of this pathogens in Peruvian children with acute respiratory infections. Methods A consecutive cross-sectional study was conducted in Lima, Peru from May 2009 to September 2010. A total of 675 children admitted with clinical diagnoses of acute respiratory infections were tested for Mycoplasma pneumoniae and Chlamydia pneumoniae detection by polymerase chain reaction (PCR), and clinical symptoms were registered by the attending physician. Results Mycoplasma pneumonia was detected in 25.19% (170/675) of nasopharyngeal samples and Chlamydia pneumonia in 10.52% (71/675). The most common symptoms in patients with these atypical pathogens were rhinorrhea, cough and fever. A higher prevalence of Mycoplasma pneumoniae cases were registered in summer, between December 2009 and March 2010. Conclusions Mycoplasma pneumoniae and Chlamydia pneumonia are a significant cause of morbidity in Peruvian children with acute respiratory infections (ARI). Further studies should evaluate the use of reliable techniques such as PCR in Peru in order to avoid underdiagnoses of these atypical pathogens. PMID:28129377

  16. The RuvA homologues from Mycoplasma genitalium and Mycoplasma pneumoniae exhibit unique functional characteristics.

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    Marcel Sluijter

    Full Text Available The DNA recombination and repair machineries of Mycoplasma genitalium and Mycoplasma pneumoniae differ considerably from those of gram-positive and gram-negative bacteria. Most notably, M. pneumoniae is unable to express a functional RecU Holliday junction (HJ resolvase. In addition, the RuvB homologues from both M. pneumoniae and M. genitalium only exhibit DNA helicase activity but not HJ branch migration activity in vitro. To identify a putative role of the RuvA homologues of these mycoplasmas in DNA recombination, both proteins (RuvA(Mpn and RuvA(Mge, respectively were studied for their ability to bind DNA and to interact with RuvB and RecU. In spite of a high level of sequence conservation between RuvA(Mpn and RuvA(Mge (68.8% identity, substantial differences were found between these proteins in their activities. First, RuvA(Mge was found to preferentially bind to HJs, whereas RuvA(Mpn displayed similar affinities for both HJs and single-stranded DNA. Second, while RuvA(Mpn is able to form two distinct complexes with HJs, RuvA(Mge only produced a single HJ complex. Third, RuvA(Mge stimulated the DNA helicase and ATPase activities of RuvB(Mge, whereas RuvA(Mpn did not augment RuvB activity. Finally, while both RuvA(Mge and RecU(Mge efficiently bind to HJs, they did not compete with each other for HJ binding, but formed stable complexes with HJs over a wide protein concentration range. This interaction, however, resulted in inhibition of the HJ resolution activity of RecU(Mge.

  17. The RuvA homologues from Mycoplasma genitalium and Mycoplasma pneumoniae exhibit unique functional characteristics.

    Science.gov (United States)

    Sluijter, Marcel; Estevão, Silvia; Hoogenboezem, Theo; Hartwig, Nico G; van Rossum, Annemarie M C; Vink, Cornelis

    2012-01-01

    The DNA recombination and repair machineries of Mycoplasma genitalium and Mycoplasma pneumoniae differ considerably from those of gram-positive and gram-negative bacteria. Most notably, M. pneumoniae is unable to express a functional RecU Holliday junction (HJ) resolvase. In addition, the RuvB homologues from both M. pneumoniae and M. genitalium only exhibit DNA helicase activity but not HJ branch migration activity in vitro. To identify a putative role of the RuvA homologues of these mycoplasmas in DNA recombination, both proteins (RuvA(Mpn) and RuvA(Mge), respectively) were studied for their ability to bind DNA and to interact with RuvB and RecU. In spite of a high level of sequence conservation between RuvA(Mpn) and RuvA(Mge) (68.8% identity), substantial differences were found between these proteins in their activities. First, RuvA(Mge) was found to preferentially bind to HJs, whereas RuvA(Mpn) displayed similar affinities for both HJs and single-stranded DNA. Second, while RuvA(Mpn) is able to form two distinct complexes with HJs, RuvA(Mge) only produced a single HJ complex. Third, RuvA(Mge) stimulated the DNA helicase and ATPase activities of RuvB(Mge), whereas RuvA(Mpn) did not augment RuvB activity. Finally, while both RuvA(Mge) and RecU(Mge) efficiently bind to HJs, they did not compete with each other for HJ binding, but formed stable complexes with HJs over a wide protein concentration range. This interaction, however, resulted in inhibition of the HJ resolution activity of RecU(Mge).

  18. Difference of clinical features in childhood Mycoplasma pneumoniae pneumonia

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    Kang Jin-Han

    2010-07-01

    Full Text Available Abstract Background M. pneumoniae pneumonia (MP has been reported in 10-40% of community-acquired pneumonia cases. We aimed to evaluate the difference of clinical features in children with MP, according to their age and chest radiographic patterns. Methods The diagnosis of MP was made by examinations at both admission and discharge and by two serologic tests: the indirect microparticle agglutinin assay (≥1:40 and the cold agglutinins titer (≥1:32. A total of 191 children with MP were grouped by age: ≤2 years of age (29 patients, 3-5 years of age (81 patients, and ≥6 years of age (81 patients. They were also grouped by pneumonia pattern: bronchopneumonia group (96 patients and segmental/lobar pneumonia group (95 patients. Results Eighty-six patients (45% were seroconverters, and the others showed increased antibody titers during hospitalization. Among the three age groups, the oldest children showed the longest duration of fever, highest C-reactive protein (CRP values, and the most severe pneumonia pattern. The patients with segmental/lobar pneumonia were older and had longer fever duration and lower white blood cell (WBC and lymphocyte counts, compared with those with bronchopneumonia. The patient group with the most severe pulmonary lesions had the most prolonged fever, highest CRP, highest rate of seroconverters, and lowest lymphocyte counts. Thrombocytosis was observed in 8% of patients at admission, but in 33% of patients at discharge. Conclusions In MP, older children had more prolonged fever and more severe pulmonary lesions. The severity of pulmonary lesions was associated with the absence of diagnostic IgM antibodies at presentation and lymphocyte count. Short-term paired IgM serologic test may be mandatory for early and definitive diagnosis of MP.

  19. Dendritic Cells from Aged Subjects Display Enhanced Inflammatory Responses to Chlamydophila pneumoniae

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    Sangeetha Prakash

    2014-01-01

    Full Text Available Chlamydophila pneumoniae (CPn is a common respiratory pathogen that causes a chronic and persistent airway infection. The elderly display an increased susceptibility and severity to this infection. However, the underlying mechanisms are not well understood. Dendritic cells (DCs are the initiators and regulators of immune responses. Therefore, we investigated the role of DCs in the age-associated increased CPn infection in vitro in humans. Though the expression of activation markers was comparable between the two age groups, DCs from aged subjects secreted enhanced levels of proinflammatory mediators such as TNF-α and CXCL-10 in response to CPn. In contrast, the secretion of IL-10 and innate interferons, IFN-α and IFN-λ, was severely impaired in DCs from aged donors. The increased activation of DCs from aged subjects to CPn also resulted in enhanced proliferation of CD4 and CD8 T cells in a DC-T coculture. Furthermore, T cells primed with CPn-stimulated DCs from aged subjects secreted increased levels of IFN-γ and reduced levels of IL-10 compared to DCs obtained from young subjects. In summary, DCs from the elderly displayed enhanced inflammatory response to CPn which may result in airway remodeling and increase the susceptibility of the elderly to respiratory diseases such as asthma.

  20. Correlation between radiological and pathological findings in patients with Mycoplasma pneumoniae pneumonia

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    Hiroshi eTanaka

    2016-05-01

    Full Text Available Studies focused on the pathological-radiological correlation of human Mycoplasma (M pneumoniae pneumonia have rarely been reported. Therefore, we extensively reviewed the literature regarding pathological and radiological studies of Mycoplasma pneumonia, and compared findings between open lung biopsy specimen and computed tomography (CT. Major three correlations were summarized. 1 Peribronchial and perivascular cuffing characterized by mononuclear cells infiltration was correlated with bronchovascular bundles thickening on CT, which was the most common finding of this pneumonia. 2 Cellular bronchitis in the small airways accompanied with exudates or granulation tissue in the lumen revealed as centrilobular nodules on CT. 3 Neutrophils and exudates in the alveolar lumen radiologically demonstrated as air-apace consolidation or ground-glass opacities. In M.pulmonis-infected mice model, pathologic patterns are strikingly different according to host cell-mediated immunity (CMI levels; treatment with interleukin-2 lead to marked cellular bronchitis in the small airways and treatment with prednisolone or cyclosporin-A lead to neutrophils and exudates in the alveolar lumen. Patients with centrilobular nodules predominant radiologic pattern have a high level of CMI, measuring by tuberculin skin test. From these findings, up-regulation of host CMI could change radiological pattern to centrilobular nodules predominant, on the other hand down-regulation of host CMI would change radiological pattern to ground-glass opacity and consolidation. It was suggested the pathological features of M. pneumoniae pneumonia may be altered by the level of host CMI.

  1. Utility of serum ferritin and lactate dehydrogenase as surrogate markers for steroid therapy for Mycoplasma pneumoniae pneumonia.

    Science.gov (United States)

    Kawamata, Ryou; Yokoyama, Koji; Sato, Miori; Goto, Masahide; Nozaki, Yasuyuki; Takagi, Takeshi; Kumagai, Hideki; Yamagata, Takanori

    2015-11-01

    Patients with severe mycoplasma pneumonia having very high serum interleukin-18 levels may require systemic corticosteroid treatment. However, we know of no laboratory markers that have been identified to assess the precise severity of Mycoplasma pneumoniae pneumonia. Thus, we investigated the usefulness of four clinical laboratory tests as severity indicators and surrogate markers for initiation of steroid therapy in these patients. For 22 Japanese children (including 3 patients who needed systemic corticosteroid therapy) diagnosed with Mycoplasma pneumoniae pneumonia, white blood cell counts and serum concentrations of interleukin-18, C-reactive protein, lactate dehydrogenase, and ferritin were determined in the acute and recovery phases. In total, 8 and 14 patients were classified as moderate and mild pneumonia, respectively, according to clinical manifestations. The serum interleukin-18 level in the acute phase of the pneumonia group was significantly higher than that of age-matched controls. Furthermore, serum interleukin-18, lactate dehydrogenase and ferritin levels in the acute phase increased in parallel with the severity of the pneumonia. The serum ferritin level was also higher in the acute phase than in the recovery phase. Positive correlations between the levels of serum interleukin-18, lactate dehydrogenase and ferritin were observed in the acute phase. Serum lactate dehydrogenase and ferritin levels may be useful as indicators of the severity of pediatric Mycoplasma pneumoniae pneumonia for initiation of corticosteroid therapy. Copyright © 2015 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

  2. Infección por Mycoplasma pneumoniae: tres casos con complicaciones neurológicas

    OpenAIRE

    Cunha, J; Madalena, C.; Guimarães, P.; Sousa, A; Temudo, T

    2002-01-01

    Summary. Introduction. Mycoplasma pneumoniae infection has been associated with severe central nervous system diseases. The pathogenesis of these disorders is unknown and the treatment uncertain. Case reports. The authors present three cases of central nervous system diseases: acute transverse myelitis, cerebellitis and encephalomyelitis associated with M. pneumoniae infection. Conclusions. M. pneumoniae infection should be considered in all cases of severe acute central nervous s...

  3. Successful treatment of Chlamydophila pneumoniae acute respiratory distress syndrome with extracorporeal membrane oxygenator: a case report and diagnostic review

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    De Bels David

    2012-01-01

    Full Text Available Abstract Introduction Chlamydophila pneumoniae is a respiratory pathogen known to infect the upper and lower respiratory tracts. Infection severity can range from sub-clinical pulmonary infection to acute respiratory distress syndrome. Case presentation A previously healthy 62-year-old Caucasian man was admitted to our hospital for acute respiratory failure. Serum samples obtained every week starting from the day of admission showed clear-cut seroconversion for C. pneumoniae antibodies. All other cultures obtained during the first days of hospitalization were negative. Despite maximal ventilatory support (high positive end expiratory pressure, fraction of inspired oxygen of 1.0, nitric oxide inhalation, neuromuscular blocking agents and prone positioning, our patient remained severely hypoxemic, which led us to initiate an extracorporeal membrane oxygenation treatment. Extracorporeal membrane oxygenation and hemodiafiltration were withdrawn on day 12. Our patient was extubated on day 18 and discharged from our Intensive Care Unit on day 20. He went home a month later. Conclusion We describe the first published case of acute respiratory distress syndrome due to C. pneumoniae infection successfully treated by extracorporeal membrane oxygenation, a very useful tool in this syndrome. A quick and specific method for the definite diagnosis of Chlamydophila infection should be developed.

  4. Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography.

    OpenAIRE

    Leith, D K; Baseman, J B

    1984-01-01

    A 165,000-dalton surface protein of Mycoplasma pneumoniae, designated protein P1, appears to be the major attachment ligand of the pathogen. We employed monoclonal antibody affinity chromatography to obtain purified protein P1.

  5. Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection.

    Science.gov (United States)

    Borel, Nicole; Mukhopadhyay, Sanghamitra; Kaiser, Carmen; Sullivan, Erin D; Miller, Richard D; Timms, Peter; Summersgill, James T; Ramirez, Julio A; Pospischil, Andreas

    2006-10-19

    Chlamydophila pneumoniae infection has been implicated as a potential risk factor for atherosclerosis, however the mechanism leading to persistent infection and its role in the disease process remains to be elucidated. We validated the use of tissue microarray (TMA) technology, in combination with immunohistochemistry (IHC), to test antibodies (GroEL, GroES, GspD, Ndk and Pyk) raised against differentially expressed proteins under an interferon-gamma (IFN-gamma) induced model of chlamydial persistence. In the cell pellet array, we were able to identify differences in protein expression patterns between untreated and IFN-gamma treated samples. Typical, large chlamydial inclusions could be observed in the untreated samples with all antibodies, whereas the number of inclusions were decreased and were smaller and atypical in shape in the IFN-gamma treated samples. The staining results obtained with the TMA method were generally similar to the changes observed between normal and IFN-gamma persistence using proteomic analysis. Subsequently, it was shown in a second TMA including archival atheromatous heart tissues from 12 patients undergoing heart transplantation, that GroEL, GroES, GspD and Pyk were expressed in atheromatous heart tissue specimens as well, and were detectable morphologically within lesions by IHC. TMA technology proved useful in documenting functional proteomics data with the morphologic distribution of GroEL, GroES, GspD, Ndk and Pyk within formalin-fixed, paraffin-embedded cell pellets and tissues from patients with severe coronary atherosclerosis. The antibodies GroEL and GroES, which were upregulated under persistence in proteomic analysis, displayed positive reaction in atheromatous heart tissue from 10 out of 12 patients. These may be useful markers for the detection of persistent infection in vitro and in vivo.

  6. Tissue MicroArray (TMA analysis of normal and persistent Chlamydophila pneumoniae infection

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    Timms Peter

    2006-10-01

    Full Text Available Abstract Background Chlamydophila pneumoniae infection has been implicated as a potential risk factor for atherosclerosis, however the mechanism leading to persistent infection and its role in the disease process remains to be elucidated. Methods We validated the use of tissue microarray (TMA technology, in combination with immunohistochemistry (IHC, to test antibodies (GroEL, GroES, GspD, Ndk and Pyk raised against differentially expressed proteins under an interferon-gamma (IFN-γ induced model of chlamydial persistence. Results In the cell pellet array, we were able to identify differences in protein expression patterns between untreated and IFN-γ treated samples. Typical, large chlamydial inclusions could be observed in the untreated samples with all antibodies, whereas the number of inclusions were decreased and were smaller and atypical in shape in the IFN-γ treated samples. The staining results obtained with the TMA method were generally similar to the changes observed between normal and IFN-γ persistence using proteomic analysis. Subsequently, it was shown in a second TMA including archival atheromatous heart tissues from 12 patients undergoing heart transplantation, that GroEL, GroES, GspD and Pyk were expressed in atheromatous heart tissue specimens as well, and were detectable morphologically within lesions by IHC. Conclusion TMA technology proved useful in documenting functional proteomics data with the morphologic distribution of GroEL, GroES, GspD, Ndk and Pyk within formalin-fixed, paraffin-embedded cell pellets and tissues from patients with severe coronary atherosclerosis. The antibodies GroEL and GroES, which were upregulated under persistence in proteomic analysis, displayed positive reaction in atheromatous heart tissue from 10 out of 12 patients. These may be useful markers for the detection of persistent infection in vitro and in vivo.

  7. Reação leucemoide e anemia hemolítica grave causada por Mycoplasma pneumoniae Leukemoid reaction and severe hemolytic anemia due to Mycoplasma pneumoniae

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    Kléber G. Luz

    2010-02-01

    Full Text Available Hemólise massiva é uma manifestação rara da infecção por Mycoplasma pneumoniae.É desencadeada por crioaglutininas, anticorpos IgM, que surgem sete a dez dias após a infecção em cerca de 50% a 75% dos casos. Hiperleucocitose é também evento incomum e orienta o diagnóstico para etiologia bacteriana ou neoplásica. Relatamos um caso de um homem de 67 anos com pneumonia por Mycoplasma pneumoniae, que apresentou anemia grave e reação leucemoide, diagnosticada por meio do teste de crioaglutininas à beira do leito e dosagem do título de anticorpo anti-mycoplasma. Após início do tratamento adequado, houve melhora importante da anemia e a leucometria foi reduzida de 56.100/mm³ para valores próximos do normal.Massive hemolysis, a rare manifestation of Mycoplasma pneumoniae infection, is due to cold agglutinins (IgM antibodies that appear seven to ten days after the infection in around 50% to 70% of cases. Hyperleukocytosis, suggestive of bacterial or neoplastic etiologies, is also an uncommon event. We report here on the case of a 67-year-old man with Mycoplasma pneumoniae pneumonia who presented with severe anemia and leukemoid reaction as diagnosed by the bedside cold agglutinin test and measurement of anti-mycoplasma antibodies. After beginning appropriate treatment, the anemia improved significantly and the leukocyte count reduced from 56100 /mm³ to close to normal level.

  8. Mycoplasma pneumoniae and Chlamydia spp. infection in community-acquired pneumonia, Germany, 2011-2012.

    Science.gov (United States)

    Dumke, Roger; Schnee, Christiane; Pletz, Mathias W; Rupp, Jan; Jacobs, Enno; Sachse, Konrad; Rohde, Gernot

    2015-03-01

    Mycoplasma pneumoniae and Chlamydia spp., which are associated with community-acquired pneumonia (CAP), are difficult to propagate, and can cause clinically indistinguishable disease patterns. During 2011-2012, we used molecular methods to test adult patients in Germany with confirmed CAP for infection with these 2 pathogens. Overall, 12.3% (96/783) of samples were positive for M. pneumoniae and 3.9% (31/794) were positive for Chlamydia spp.; C. psittaci (2.1%) was detected more frequently than C. pneumoniae (1.4%). M. pneumoniae P1 type 1 predominated, and levels of macrolide resistance were low (3.1%). Quarterly rates of M. pneumoniae-positive samples ranged from 1.5% to 27.3%, showing a strong epidemic peak for these infections, but of Chlamydia spp. detection was consistent throughout the year. M. pneumoniae-positive patients were younger and more frequently female, had fewer co-occurring conditions, and experienced milder disease than did patients who tested negative. Clinicians should be aware of the epidemiology of these pathogens in CAP.

  9. Chlamydia pneumoniae and mycoplasma pneumoniae in children with acute respiratory infection in general practices in the Netherlands.

    NARCIS (Netherlands)

    Tjhie, J.H.T.; Dorigo-Zetsma, J.W.; Roosendaal, R.; Brule, A.J.C. van den; Bestebroer, T.M.; Bartelds, A.I.M.; Vandenbroucke-Grauls, C.M.J.E.

    2000-01-01

    In this retrospective study Chlamydia pneumoniae and Mycoplasma pneumoniae infections were detected by polymerase chain reaction (PCR) in samples (n=457) from children presenting with acute respiratory infection to general practitioners during 1992-97. Samples were collected in autumn and winter, an

  10. Mycoplasma pneumoniae pneumonia in hospitalized children diagnosed at acute stage by paired sera

    Institute of Scientific and Technical Information of China (English)

    LIU Chun-ling; WEI Ming; LIU Zhen-ye; WANG Gui-qiang; ZHANG Bo; XU Hua; HU Liang-ping; HE Xiao-feng; WANG Jun-hua; ZHANG Jun-hong; LIU Xiao-yu

    2010-01-01

    Background Mycoplasma pneumoniae (M. pneumoniae) is a frequent cause of respiratory tract infections. However,there is deficient knowledge about the clinical manifestations of M. pneumoniae infection. We described the clinical and laboratory findings of M. pneumoniae pneumonia in hospitalized children who were all diagnosed by a ≥ fourfold increase in antibody titer.Methods M. pneumoniae antibodies were routinely detected in children admitted with acute respiratory infection during a one-year period. The medical history was re-collected from children whose M. pneumoniae antibody titer increased≥fourfold at the bedside by a single person, and their frozen paired serum samples were measured again for the M.pneumoniae antibody titer.Results Of the 635 children whose sera were detected for the M. pneumoniae antibody, paired sera were obtained from 82 and 29.3% (24/82) showed a ≥ fourfold increase in antibody titer. There were 24 cases, nine boys and 15 girls, aged from two to 14 years, whose second serum samples were taken on day 9 at the earliest after symptom onset; the shortest interval was three days. All children presented with a high fever (≥38.5℃) and coughing. Twenty-one had no nasal obstruction or a runny nose, and five had mild headaches which all were associated with the high fever. The disease was comparatively severe if the peak temperature was >39.5℃. All were diagnosed as having pneumonia through chest X-rays. Four had bilateral or multilobar involvement and their peak temperatures were all ≤ 39.5℃. None of the children had difficulty in breathing and all showed no signs of wheezing.Conclusions The second serum sample could be taken on day 9 at the earliest after symptom onset meant that paired sera could be used for the clinical diagnosis of M. pneumoniae pneumonia in children at the acute stage. M. pneumoniae is a lower respiratory tract pathogen. Extrapulmonary complications were rare and minor in our study. High peak temperature (

  11. Effect of the termini of RNase Hs from Chlamydophila pneumoniae on enzymatic biochemical characterization

    Institute of Scientific and Technical Information of China (English)

    Jingli Hou; Zheng Lu; Xingliang Guo; Jianhua Liu

    2012-01-01

    A difference between prokaryotic RNase HⅡ and HⅢ,which both belong to type 2 RNase H,is a long N-terminal extension of HⅢ; however,the main-fold structures of HⅡ and HⅢ known as RNase H-fold are similar.To further understand the structure-function relationship of RNase HⅡ and RNase HⅢ,biochemical analyses were carried out using N-terminal truncations of RNase HⅢ (ⅢN56Δ,ⅢN81Δ,and ⅢN88Δ) and C-terminal truncation (ⅡC19Δ) of RNase HⅡ from Chlamydophila pneumoniae.Compared with wild-type CpRNase HⅡ/Ⅲ,IIIN56Δhad no obvious variation on the cleavage site and efficiency of DNA-rN1-DNA/DNA (DR1D) and DNA-rN4-DNA/DNA (DR4D) substrates.ⅡC19Δ and ⅢN81Δ both showed decreased activities,and ⅢN88Δ exhibited little cleavage on these substrates.However,ⅢN81Δ showed very different activities toward different substrates (20%for DR1D and 85% for DR4D).Moreover,ⅡC19ΔⅢN82-88 mutant,prepared through adding N-terminal 82nd to 88th residues locating at the bound region of N-and C-terminal domains of CpRNase HⅢ to N-terminus of ⅡC19Δ,cleaved DR4D substrate more efficiently and preferentially at the cleavage sites of CpRNase HⅢ but not those of CpRNase HⅡ.These results indicated that C-termini of CpRNase HⅡ,N-termini of CpRNase HⅢ,and bound region of N-and C-terminal domain are all important for enzymatic activities.Moreover,the 82nd to 88th residues of N-terminus of CpRNase HⅡ are related with enzyme cleavage site specificity.These results will help to understand the importance of C-termini of CpRNase HⅡ and N-termini of CpRNase HⅢ to the enzyme activities for DR1D and DR4D substrate.

  12. Acute Chlamydia pneumoniae and Mycoplasma pneumoniae infections in community-acquired pneumonia and exacerbations of COPD or asthma: therapeutic considerations.

    Science.gov (United States)

    Meloni, F; Paschetto, E; Mangiarotti, P; Crepaldi, M; Morosini, M; Bulgheroni, A; Fietta, A

    2004-02-01

    Rates of acute Chlamydia pneumoniae and Mycoplasma pneumoniae infections were determined in 115 adults hospitalized for community-acquired pneumonia (CAP), purulent exacerbations of COPD and acute exacerbations of bronchial asthma, by means of serology and molecular methods. Results were compared with those obtained in a matched control group. Common respiratory pathogens were isolated by cultures in 22.5% and 22.2% of CAP and exacerbated COPD patients, respectively. Cultures from exacerbated asthma patients were always negative. Serological and molecular evidence of current C. pneumoniae infection was obtained in 10.0%, 8.9% and 3.3% of CAP, COPD and asthma cases. The corresponding rates of acute M. pneumoniae infection were 17.5%, 6.7% and 3.3%, respectively. Finally, no difference was found between typical and atypical pathogen rates. These findings highlight the importance of taking into account C. pneumoniae and M. pneumoniae infections in guiding the choice of empirical antibacterial treatment for CAP and purulent exacerbations of COPD.

  13. 儿童肺炎支原体肺炎治疗进展%The recent progress on the treatment of mycoplasma pneumoniae pneumonia in children

    Institute of Scientific and Technical Information of China (English)

    盛江吟; 张海邻; 李昌崇

    2016-01-01

    Mycoplasma pneumoniae pneumonia is one of common pneumonia in the school-age children and adolescents,as well as in infants.The mycoplasma pneumoniae infection has self-limiting,but because of the refractory mycoplasma pneumoniae pneumonia,mycoplasma pneumoniae pneumonia treatment faces enormous challenges,therefore,how to effectively treat mycoplasma pneumoniae pneumonia is of great significance for children's health.Mycoplasma pneumoniae pneumonia treatment including effective antibiotics.immune adjustment and bronchoseopy and so on,according to the different condition of patients progress to take the corresponding treatment.%肺炎支原体肺炎是学龄儿童常见的一种肺炎,婴幼儿也可发生.虽然肺炎支原体感染有一定的自限性,但近年来难治性肺炎支原体肺炎的出现,使肺炎支原体肺炎治疗面临巨大挑战,如何合理治疗肺炎支原体肺炎,对儿童健康的恢复具有重要意义.肺炎支原体肺炎的治疗包括有效抗菌药物、免疫调节及支气管镜等,应根据患儿不同的病情进展情况采取相应的治疗方案.

  14. The validity of the criteria for primary infection of Chlamydophila pneumoniae in children by measuring ELISA IgM antibodies.

    Science.gov (United States)

    Kamata, Ayako; Obinata, Kaoru; Niizuma, Takahiro; Kinoshita, Keiji; Shimizu, Toshiaki

    2012-06-01

    As IgM antibody measurement by enzyme-linked immunosorbent assay (ELISA) has become possible for the serological diagnosis of Chlamydophila pneumoniae (C. pn) infection, the HITAZYME-ELISA method has become widely employed in Japan. However, in children, when the diagnostic criterion of primary infection is set at ID ≥1.1, the positive rate is higher than expected, and the potential for inaccurate reflection of the prevalence has been raised. In this study, we performed ROC analysis involving 136 pediatric patients with acute airway symptoms (0-14 years of age), considering a 32-fold or higher micro-immunofluorescence IgM antibody titer against C. pn as positive. Setting the cut-off value for ELISA C. pn IgM antibody ID at 2.0, the specificity was 100%, with no false positivity. The maximum (sensitivity + specificity)/2 was obtained when the cut-off value was set at 1.5. Therefore, IgM ID ≥2.0 was regarded as definitely positive and an IgM ID between 1.5 and 2.0 was regarded as indeterminate as diagnostic criteria for the primary infection. When the prevalence was investigated in 3,108 children (0-15 years of age) with airway symptoms based on these criteria, 542 cases (17.4%) were positive, and the median duration of IgM antibody positivity was five months. Long-term positivity (ten cases) for more than 12 months and recurrent positivity (eight cases) were also observed, but it may be appropriate to set a new criterion of IgM antibody ID ≥2.0 for the diagnosis of primary Chlamydophila pneumoniae infection in children.

  15. Chlamydophila pneumoniae derived from inclusions late in the infectious cycle induce aponecrosis in human aortic endothelial cells

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    Groscurth Peter

    2008-02-01

    Full Text Available Abstract Background Atherosclerosis is still the leading cause of death in the western world. Besides known risk factors studies demonstrating Chlamydophila pneumoniae (C. pneumoniae to be implicated in the progression of the disease, little is known about C. pneumoniae infection dynamics. We investigated whether C. pneumoniae induce cell death of human aortic endothelial cells, a cell type involved in the initiation of atherosclerosis, and whether chlamydial spots derive from inclusions. Results Lactate dehydrogenase release revealed host cell death to be dependent on the amounts of Chlamydia used for infection. The morphology of lysed human aortic endothelial cells showed DNA strand breaks simultaneously with cell membrane damage exclusively in cells carrying Chlamydia as spots. Further ultrastructural analysis revealed additional organelle dilation, leading to the definition as aponecrotic cell death of endothelial cells. Exclusive staining of the metabolic active pathogens by chlamydial heat shock protein 60 labelling and ceramide incorporation demonstrated that the bacteria responsible for the induction of aponecrosis had resided in former inclusions. Furthermore, a strong pro-inflammatory molecule, high mobility group box protein 1, was shown to be released from aponecrotic host cells. Conclusion From the data it can be concluded that aponecrosis inducing C. pneumoniae stem from inclusions, since metabolically active bacterial spots are strongly associated with aponecrosis late in the infectious cycle in vascular endothelial cells and metabolic activity was exclusively located inside of inclusions in intact cells. Vice versa initial spot-like infection with metabolically inert bacteria does not have an effect on cell death induction. Hence, C. pneumoniae infection can contribute to atherosclerosis by initial endothelial damage.

  16. A novel transport mechanism for MOMP in Chlamydophila pneumoniae and its putative role in immune-therapy.

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    Francis O Atanu

    Full Text Available Major outer membrane proteins (MOMPs of Gram negative bacteria are one of the most intensively studied membrane proteins. MOMPs are essential for maintaining the structural integrity of bacterial outer membranes and in adaptation of parasites to their hosts. There is evidence to suggest a role for purified MOMP from Chlamydophila pneumoniae and corresponding MOMP-derived peptides in immune-modulation, leading to a reduced atherosclerotic phenotype in apoE(-/- mice via a characteristic dampening of MHC class II activity. The work reported herein tests this hypothesis by employing a combination of homology modelling and docking to examine the detailed molecular interactions that may be responsible. A three-dimensional homology model of the C. pneumoniae MOMP was constructed based on the 14 transmembrane β-barrel crystal structure of the fatty acid transporter from Escherichia coli, which provides a plausible transport mechanism for MOMP. Ligand docking experiments were used to provide details of the possible molecular interactions driving the binding of MOMP-derived peptides to MHC class II alleles known to be strongly associated with inflammation. The docking experiments were corroborated by predictions from conventional immuno-informatic algorithms. This work supports further the use of MOMP in C. pneumoniae as a possible vaccine target and the role of MOMP-derived peptides as vaccine candidates for immune-therapy in chronic inflammation that can result in cardiovascular events.

  17. A novel transport mechanism for MOMP in Chlamydophila pneumoniae and its putative role in immune-therapy.

    Science.gov (United States)

    Atanu, Francis O; Oviedo-Orta, Ernesto; Watson, Kimberly A

    2013-01-01

    Major outer membrane proteins (MOMPs) of Gram negative bacteria are one of the most intensively studied membrane proteins. MOMPs are essential for maintaining the structural integrity of bacterial outer membranes and in adaptation of parasites to their hosts. There is evidence to suggest a role for purified MOMP from Chlamydophila pneumoniae and corresponding MOMP-derived peptides in immune-modulation, leading to a reduced atherosclerotic phenotype in apoE(-/-) mice via a characteristic dampening of MHC class II activity. The work reported herein tests this hypothesis by employing a combination of homology modelling and docking to examine the detailed molecular interactions that may be responsible. A three-dimensional homology model of the C. pneumoniae MOMP was constructed based on the 14 transmembrane β-barrel crystal structure of the fatty acid transporter from Escherichia coli, which provides a plausible transport mechanism for MOMP. Ligand docking experiments were used to provide details of the possible molecular interactions driving the binding of MOMP-derived peptides to MHC class II alleles known to be strongly associated with inflammation. The docking experiments were corroborated by predictions from conventional immuno-informatic algorithms. This work supports further the use of MOMP in C. pneumoniae as a possible vaccine target and the role of MOMP-derived peptides as vaccine candidates for immune-therapy in chronic inflammation that can result in cardiovascular events.

  18. Molecular characterization of macrolide resistance of a Mycoplasma pneumoniae strain that developed during therapy of a patient with pneumonia

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    Roger Dumke

    2014-12-01

    Full Text Available The development of macrolide resistance that occurred during 3 days of therapy with azithromycin to treat Mycoplasma pneumoniae pneumonia in a paediatric patient is reported. After extended molecular characterization of strains, the parallel occurrence of clones showing the non-mutated wild-type 23S rRNA sequence as well as mutations A2063G and A2064G, which are both responsible for phenotypic resistance, was confirmed for the first time.

  19. Manifestaciones mucocutáneas debidas a la infección por Mycoplasma pneumoniae. Reporte de 3 casos

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    Beatriz Di Martino Ortiz,

    2015-07-01

    Full Text Available Mycoplasma pneumoniae, one of the most frequent agents of community-acquired pneumonia worldwide, can affect different organs besides the lung, causing skin lesions in 25% of patients. The spectrum of dermatological features include macules, papules, purpura, target lesions and even well stablished conditions as erythema multiforme, Stevens- Johnson’s Syndrome and toxical epidermal necrolysis. We present 3 pediatric patients with erythema multiforme and toxical epidermal necrolysis in which Mycoplasma pneumoniae was considered as the etiological agent.

  20. Late lung parenchymal changes on HRCT in children with mycoplasma pneumonia

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Soo Hyeon; Kim, Joung Sook; Yoon, Jung Hee; Hur, Gham; Kim, Chang Gun [Inje Univ. Sanggye Paik Hospital, Seoul (Korea, Republic of)

    1999-08-01

    To evaluate late lung parenchymal change, as seen on high-resolution CT(HRCT) in children with mycoplasma pneumonia. Twenty-three patients [15 boys and 8 girls aged two to 13 (mean, 6) years] with mycoplasma pneumonia underwent HRCT four to 39 (mean, 10) months after initial infection. Using increased mycoplasma antibody titer( > 1;640) mycoplasma pneumonia was diagnosed, and patients were divided into two groups : high titer group (antibody titer > 1:5120), and lower titer group ( < 1:5120). CT scans were performed using 2mm collimation and 5-10mm interval from apex to diaphragm. In seven patients who were cooperative, both inspiratory scans were obtained at a window width of 1600 HU and level of 700. HRCT findings of mosaic low attenuations and changes in bronchioles and bronchial walls were assessed by three radiologists and correlated with initial chest radiographic findings. On HRCT, 17 of 23 patients (74%) demonstrated abnormal findings. These included mosaic attenuation of lung density alone in 11 of 17 patients (65%), mosaic attenuation associated with bronchiectasis in five(29%), and bronchiectasis only in one(6%). Mosaic attenuation was more accentuated on expiratory scans than on inspiratory. These findings were obtained in 10 of 12 high titer group and in 7 of 11 in the lower titer group. In 15 of 23 patients(65%), involved areas seen on HRCT exactly corresponded with initially involved areas seen on chest radiographs (CXR). Two patients in whom findings on initial CXR were normal showed mosaic attenuation on HRCT. Six patients in whom such findings were abnormal showed normal findings on HRCT, a fact which reflected their complete recovery. The most common late parenchymal change in mycoplasma pneumonia, as seen on HRCT, was mosaic attenuation of lung density followed by bronchiectasis. The latter is presumably due to bronchiolitis obliterans, a well-known complication. We believe that HRCT is very useful for the evaluation of long-term sequelae of

  1. International Mycoplasma pneumoniae typing study: interpretation of M. pneumoniae multilocus variable-number tandem-repeat analysis

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    V.J. Chalker

    2015-09-01

    Full Text Available Typing of Mycoplasma pneumoniae by multiple-locus variable-number tandem repeat analysis (MLVA is increasingly in use. However, no specific internationally agreed guidance is available. Thirty M. pneumoniae DNA samples including serial dilutions of a type strain were sent to six international laboratories to perform MLVA and results were compared. Good correlation was observed, indicating that this methodology can be robustly performed in multiple sites. However, differences due to interpretation of fragment size, repeat sequence identification and repeat numbering led to inconsistency in the final profiles assigned by laboratories. We propose guidelines for interpreting M. pneumoniae MLVA typing and assigning the number of repeats.

  2. Combined Striatum, Brain Stem, and Optic Nerve Involvement due to Mycoplasma pneumoniae in an Ambulatory Child

    Directory of Open Access Journals (Sweden)

    Jin-Won Bae

    2011-05-01

    Full Text Available In children, Mycoplasma pneumoniae encephalitis has been characterized by acute onset of an encephalopathy associated with extrapyramidal symptoms and symmetric basal ganglia with or without brain stem involvement on magnetic resonance imaging. Our case, showing unilateral optic neuritis, ophthalmoplegia, no extrapyramidal symptoms, and typical striatal involvement on magnetic resonance imaging, broadens the spectrum of varying clinical manifestations of childhood M. pneumoniae-associated encephalopathy.

  3. Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling

    NARCIS (Netherlands)

    Wodke, J.A.; Puchalka, J.; Lluch-Senar, M.; Marcos, J.; Yus, E.; Godinho, M.; Gutierrez-Gallego, R.; Martins Dos Santos, V.A.P.; Serrano, L.; Klipp, E.; Maier, T.

    2013-01-01

    Mycoplasma pneumoniae, a threatening pathogen with a minimal genome, is a model organism for bacterial systems biology for which substantial experimental information is available. With the goal of understanding the complex interactions underlying its metabolism, we analyzed and characterized the met

  4. Detection of IgM and IgG antibodies to Chlamydophila pneumoniae in pediatric community-acquired lower respiratory tract infections

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    Surinder Kumar

    2011-01-01

    Full Text Available Context: Chlamydophila pneumoniae (C. pneumoniae is an emerging infectious agent with a spectrum of clinical manifestations including lower and upper respiratory tract infections. Aims: To investigate the role of C. pneumoniae in community-acquired lower respiratory tract infections (LRTIs in children using serological tests. Settings and Design: Two hundred children, age 2 months to 12 years, hospitalized for community-acquired LRTIs were investigated for C. pneumoniae etiology. Materials and Methods: We investigated 200 children hospitalized for community-acquired LRTIs, using ELISA for detecting anti-C. pneumoniae IgM and IgG antibodies. The demographic, clinical and radiological findings for C. pneumoniae antibody positive and C. pneumoniae antibody negative cases were compared. Statistical Analysis Used: Data analysis was performed by Chi-square test and Fisher′s exact tests using Epi Info (2002. Results: Clinical and radiological findings in both the groups were comparable. Serological evidence of C. pneumoniae infection was observed in 12 (6% patients; specific IgM antibodies were detected in 11 (91.67%; specific IgG antibodies in 1 (8.33% patients, while 4-fold rise in C. pneumoniae IgG antibody titers were noted in none of the patients. Conclusions: C. pneumoniae has a role in community-acquired LRTIs, even in children aged < 5 years. Serological detection using ELISA would enable pediatricians in better management of C. pneumoniae infections.

  5. Reconstitution of an active arginine deiminase pathway in Mycoplasma pneumoniae M129.

    Science.gov (United States)

    Rechnitzer, Hagai; Rottem, Shlomo; Herrmann, Richard

    2013-10-01

    Some species of the genus Mycoplasma code for the arginine deiminase pathway (ADI), which enables these bacteria to produce ATP from arginine by the successive reaction of three enzymes: arginine deiminase (ArcA), ornithine carbamoyltransferase (ArcB), and carbamate kinase (ArcC). It so far appears that independently isolated strains of Mycoplasma pneumoniae encode an almost identical truncated version of the ADI pathway in which the proteins ArcA and ArcB have lost their original enzymatic activities due to the deletion of significant regions of these proteins. To study the consequences of a functional ADI pathway, M. pneumoniae M129 was successfully transformed with the cloned functional arcA, arcB, and arcC genes from Mycoplasma fermentans. Enzymatic tests showed that while the M. pneumoniae ArcAB and ArcABC transformants possess functional arginine deiminase, ornithine carbamoyltransferase, and carbamate kinase, they were unable to grow on arginine as the sole energy source. Nevertheless, infection of a lung epithelial cell line, A549, with the M. pneumoniae transformants showed that almost 100% of the infected host cells were nonviable, while most of the lung cells infected with nontransformed M. pneumoniae were viable under the same experimental conditions.

  6. Fever-triggered Brugada syndrome in an adult patient presenting with hemophagocytic syndrome induced by Chlamydophila pneumoniae.

    Science.gov (United States)

    Vieira, Miguel Bigotte; Gaibino, Nuno; Pignatelli, Alexandra; Oliveira, Anabela

    2015-10-09

    A previously healthy 29-year-old man was admitted to our hospital, with a 4-day history of fever (>39°C), rigours, diaphoresis, fatigue and retro-orbital headache. On examination, he was febrile (37.8°C) and tachycardic (110 bpm). Laboratory work up revealed bicytopenia (white cell count 1.37×10(9)/L, platelets 60×10(9)/L) and an increase in C reactive protein (9 mg/dL). The ECG showed ST segment elevation in V1, V2 and V3 leads. The patient was admitted and investigation was initiated revealing prolonged fever (>7 days), pancytopenia, hepatosplenomegaly, hyperferritinemia, hypertriglyceridemia, hypofibrinogenemia, elevated soluble CD25 and hemophagocytosis in bone marrow. Therefore, the patient presented 7 of the 8 diagnostic criteria of hemophagocytic syndrome. Laboratorial investigation for infectious causes was negative, except for IgA and IgG Chlamydophila pneumoniae. ECG re-evaluation on the day of discharge showed no ST segment elevation and no other abnormalities. Genetic testing for known mutations associated with hemophagocytic syndrome and Brugada syndrome did not show any mutations in these genes.

  7. Lung function and bronchial responsiveness after Mycoplasma pneumoniae infection in early childhood

    DEFF Research Database (Denmark)

    Kjaer, B.B.; Jensen, J.S.; Nielsen, K.G.

    2008-01-01

    .17 versus 1.21 (kPa sec), P=0.45; and mean change in specific resistance was 13% versus 9%, P= 0.42. In conclusion, M. pneumoniae infection in early childhood was not associated with long-term effects on lung function and bronchial hyperresponsiveness 2 years after infection Udgivelsesdato: 2008/6......Mycoplasma (M.) pneumoniae has been associated with exacerbation of symptoms in asthmatic school children and adults; and an etiological role in asthma has been suggested. The purpose of this study was to investigate whether infection with M. pneumoniae in early childhood has a long-term influence...... on lung function and bronchial responsiveness. In a retrospective, clinical cohort-study children younger than 5 years-of-age when PCR-tested for M. pneumoniae were enrolled. Sixty-five children with clinical symptoms suggesting infection with M. pneumoniae during an epidemic season completed a clinical...

  8. Role of Serum Mycoplasma pneumoniae IgA, IgM, and IgG in the Diagnosis of Mycoplasma pneumoniae-Related Pneumonia in School-Age Children and Adolescents.

    Science.gov (United States)

    Lee, Wei-Ju; Huang, Eng-Yen; Tsai, Chih-Min; Kuo, Kuang-Che; Huang, Yi-Chuan; Hsieh, Kai-Sheng; Niu, Chen-Kuang; Yu, Hong-Ren

    2017-01-01

    Mycoplasma pneumoniae is an important causative pathogen of community-acquired pneumonia in children. Rapid and reliable laboratory diagnosis of M. pneumoniae infection is important so that appropriate antibiotic treatment can be initiated to reduce the misuse of drugs and resistance rates. Anti-M. pneumoniae immunoglobulin M (IgM) is an indicator of recent primary infection but can persist for several months after initial infection. It has been suggested that anti-M. pneumoniae immunoglobulin A (IgA) can be a reliable indicator for recent M. pneumoniae infection in adults. We investigated the clinical diagnostic value of M. pneumoniae IgA in school-age children and adolescents with M. pneumoniae-related pneumonia. Eighty children with pneumonia and seropositive for M. pneumoniae IgM or with a 4-fold increase of anti-M. pneumoniae immunoglobulin G (IgG) were enrolled from May 2015 to March 2016. The titers of M. pneumoniae IgA, IgM, and IgG, the clinical features, and laboratory examinations of blood, C-reactive protein, and liver enzymes were analyzed. The initial positivity rates for M. pneumoniae IgM and IgA upon admission to the hospital were 63.6 and 33.8%, respectively. One week after admission, the cumulative positivity rates for M. pneumoniae IgM and IgA increased to 97.5 and 56.3%, respectively. Detection of M. pneumoniae IgM was more sensitive than detection of M. pneumoniae IgA for the diagnosis of M. pneumoniae-related pneumonia in school-age children and adolescents; however, paired sera are necessary for a more accurate diagnosis.

  9. Application of serology and nested polymerase chain reaction for identifying Chlamydophila pneumoniae in community-acquired lower respiratory tract infections in children

    Directory of Open Access Journals (Sweden)

    Surinder Kumar

    2016-01-01

    Full Text Available Context: Chlamydophila pneumoniae is a common cause of community-acquired respiratory infections, including pneumonia, bronchitis, and upper respiratory tract infections. Since it is difficult to detect C. pneumoniae in clinical practice, specific etiological diagnosis is established only in a minority of cases. Aims: To investigate the role of C. pneumoniae in community-acquired lower respiratory tract infections (LRTIs in children, with the use of serological tests and nested polymerase chain reaction (PCR analysis. Settings and Design: One hundred children, age of 2 months to 12 years, hospitalized for community-acquired LRTIs were investigated for C. pneumoniae etiology. Materials and Methods: We investigated 100 children hospitalized for community-acquired LRTIs, using enzyme-linked immunosorbent assay for detecting anti-C. pneumoniae immunoglobulin M, and immunoglobulin G antibodies and nasopharyngeal aspirates for analysis of C. pneumoniae PCR. The demographic, clinical, and radiological findings for C. pneumoniae antibody positive and C. pneumoniae antibody negative cases were compared. Statistical Analysis Used: Data analysis was performed by Chi-square test and Fisher′s exact tests using Epi Info (2002. Results: Clinical and radiological findings in both the groups were comparable. A relatively higher rate of C. pneumoniae infection in children was observed below 5 years of age. Serological evidence of C. pneumoniae infection was observed in 12 (12% patients and nested PCR was positive in 5 (5% children. Thirteen (13% patients were diagnosed with C. pneumoniae infection by serology and/or nested PCR. Conclusions: Our study confirms that C. pneumoniae plays a significant role in community-acquired LRTIs in children of all ages, even in children aged <5 years.

  10. Biomarker Candidates of Chlamydophila pneumoniae Proteins and Protein Fragments Identified by Affinity-Proteomics Using FTICR-MS and LC-MS/MS

    Science.gov (United States)

    Susnea, Iuliana; Bunk, Sebastian; Wendel, Albrecht; Hermann, Corinna; Przybylski, Michael

    2011-04-01

    We report here an affinity-proteomics approach that combines 2D-gel electrophoresis and immunoblotting with high performance mass spectrometry to the identification of both full length protein antigens and antigenic fragments of Chlamydophila pneumoniae (C. pneumoniae). The present affinity-mass spectrometry approach effectively utilized high resolution FTICR mass spectrometry and LC-tandem-MS for protein identification, and enabled the identification of several new highly antigenic C. pneumoniae proteins that were not hitherto reported or previously detected only in other Chlamydia species, such as Chlamydia trachomatis. Moreover, high resolution affinity-MS provided the identification of several neo-antigenic protein fragments containing N- and C-terminal, and central domains such as fragments of the membrane protein Pmp21 and the secreted chlamydial proteasome-like factor (Cpaf), representing specific biomarker candidates.

  11. A case series of urticaria multiforme: Its association with Mycoplasma pneumoniae infection?

    Directory of Open Access Journals (Sweden)

    Yakup Canıtez

    2015-06-01

    Full Text Available Urticaria multiforme is a rare clinical and morphological subtype of acute urticaria in childhood. Clinical features include acute onset of blanchable, annular, polycyclic, erythematous wheals with dusky or ecchymotic centers (target lesion-like. Pruritus in nearly all and edema on the face, hand and foot in the majority of cases are seen. Urticaria multiforme known as a cutaneous hypersensitivity reaction can often be misdiagnosed as erythema multiforme and serum-sickness-like reaction. Although the majority of cases have been related with viral or bacterial infections and drug use, the exact etiopathogenesis of the disease is yet unclear. Association of Mycoplasma pneumoniae infection and urticaria multiforme have been reported in only two cases in the literature. In this article, a total of four cases diagnosed as urticaria multiforme two of whom had clinical and serological proof of Mycoplasma pneumoniae infection has been presented, and diagnostic criteria, differential diagnosis, etiopathogenesis and treatment of urticaria multiforme have been reviewed.

  12. Effect of Yanhuning on the serum inflammatory cytokines and immunological function of children with mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    Xiu-Mei Zhong; Yan Deng; Xue-Mei Chu

    2015-01-01

    Objective: To probe the effect of Yanhuning on the Serum inflammatory cytokines and immunological function of children with mycoplasma pneumonia. Method: 110 children with MP were randomly divined into observed group (treated with azithromycin and Yanhuning) and control group (only treated with azithromycin) with 55 cases respectively. Results:Compared with those before treatment, serum levels of inflammatory cytokines (TNF-α, IL-6, IFN-γ, IL-2, IL-13) and immune function indicators (CD4+, IgG) of the two groups all have significant difference. When compared with those of control group, serum levels of inflammatory cytokines including TNF-α, IL-6, IFN-γ, IL-2, IL-13 and immune function indicators of CD4+ all have significant difference, while IgG level has no significant difference. Conclusions: Yanhuning has significant curative effect on the treatment of children with mycoplasma pneumonia and can effectively improve patients' serum inflammatory factors and immunological function indexes.

  13. Mycoplasma pneumonia associated with rhabdomyolysis and the Guillain-Barre syndrome.

    Science.gov (United States)

    Gupta, R; Gupta, A; Goyal, V; Guleria, R; Kumar, A

    2005-01-01

    A 25-year-old housewife who presented with Mycoplasma pneumonia who developed acute respiratory distress syndrome (ARDS) and required assisted ventilation. During her hospital stay, she developed acute renal failure because of rhabdomyolysis and was put on haemodialysis. She also had difficulty in weaning from ventilator because of acute motor-sensory axonal neuropathy (AMSAN) variant of the Guillain-Barre syndrome. The patient was treated with antibiotics and corticosteroids. The patient recovered from both the complications gradually.

  14. Mycoplasma pneumoniae: Current knowledge on nucleic acid amplification techniques and serological diagnostics

    Directory of Open Access Journals (Sweden)

    Katherine eLoens

    2016-03-01

    Full Text Available Mycoplasma pneumoniae (M. pneumoniae belongs to the class Mollicutes and has been recognized as a common cause of respiratory tract infections (RTIs, including community-acquired pneumonia (CAP, that occur worldwide and in all age groups. In addition, M. pneumoniae can simultaneously or sequentially lead to damage in the nervous system and has been associated with a wide variety of other acute and chronic diseases. During the past 10 years, the proportion of LRTI in children and adults, associated with M. pneumoniae infection has ranged from 0% to more than 50%. This variation is due to the age and the geographic location of the population examined but also due to the diagnostic methods used. The true role of M. pneumoniae in RTIs remains a challenge given the many limitations and lack of standardization of the applied diagnostic tool in most cases, with resultant wide variations in data from different studies.Correct and rapid diagnosis and/or management of M. pneumoniae infections is, however, critical to initiate appropriate antibiotic treatment and is nowadays usually done by PCR and/or serology. Several recent reviews have summarized current methods for the detection and identification of M. pneumoniae. This review will therefore provide a look at the general principles, advantages, diagnostic value, and limitations of the most currently used detection techniques for the etiological diagnosis of a M. pneumoniae infection as they evolve from research to daily practice.

  15. Transient cold agglutinins associated with Mycoplasma cynos pneumonia in a dog.

    Science.gov (United States)

    Pinkos, Alyssa C; Friedrichs, Kristen R; Monaghan, Kelly N; Sample, Saundra H; Trepanier, Lauren A

    2015-12-01

    This report details a case of reversible cold agglutinins in a dog with Mycoplasma cynos pneumonia. An 11-month-old female spayed Rhodesian Ridgeback was presented for lethargy and cough. Thoracic radiographs revealed an alveolar pattern present bilaterally in the cranioventral lung lobes. Septic neutrophilic inflammation with suspected Mycoplasma sp. organisms was noted on cytologic examination of a trans-tracheal wash, and the dog was treated empirically with IV ampicillin/sulbactam and enrofloxacin pending culture results. Red blood cell agglutination was noted unexpectedly on several blood film reviews during hospitalization; however, the dog never developed clinical or laboratory evidence of hemolysis. Cold agglutinins were demonstrated based on the results of a saline dilution and cold agglutinin test that showed agglutination at 4°C but not at room temperature (21°C) or 37°C. Based on a positive culture for M cynos, the dog was treated for 8 weeks with oral enrofloxacin. After clinical and radiographic resolution of the pneumonia, repeated saline dilution and cold agglutinin tests of peripheral blood were negative at all temperatures. Reversible, asymptomatic cold agglutinins are common in human patients with mycoplasma pneumonia, but this is the first reported case in a dog.

  16. Mycoplasma pneumoniae: infección vía aérea superior

    OpenAIRE

    Bernal M,Jose; Bogado C,Mariana; Bernal R,Jose; Fuenzalida C,Loreto

    2006-01-01

    Introducción. Las infecciones agudas de la vía aérea alta (IRAs) son un problema común de consulta médica. Su clínica inicial es ¡nespecífica, dificultando la determinación de un agente etiológico. Estudios revelan predominio etiológico viral, sin embargo, investigaciones recientes demuestran que bacterias atípicas, principalmente Mycoplasma pneumoniae, tienen importante rol como causa de IRAs. El diagnóstico de infección por M pneumoniae se realiza por métodos directos (cultivo y/o RCP) o in...

  17. Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells

    DEFF Research Database (Denmark)

    Drasbek, Mette; Christiansen, Gunna; Drasbek, Kim Ryun;

    2007-01-01

    The human pathogen Mycoplasma pneumoniae can cause atypical pneumonia through adherence to epithelial cells in the respiratory tract. The major immunogenic protein, P1, participates in the attachment of the bacteria to the host cells. To investigate the adhesion properties of P1, a recombinant...... protein (rP1-II) covering amino acids 1107-1518 of the P1 protein was produced. This protein inhibited the adhesion of M. pneumoniae to human HEp-2 cells, as visualized in a competitive-binding assay using immunofluorescence microscopy. Previous studies have shown that mAbs that recognize two epitopes...... intensity. The number of M. pneumoniae microcolonies adhering to the host cells was significantly reduced by these five peptides. Further investigations showed that inhibiting peptide 7 (amino acids 1347-1396) of the major adhesin protein P1 bound directly to host receptors, suggesting that the observed M...

  18. Carriage of Mycoplasma pneumoniae in the Upper Respiratory Tract of Symptomatic and Asymptomatic Children: An Observational Study

    NARCIS (Netherlands)

    E.B.M. Spuesens (Emiel); P.L.A. Fraaij (Pieter); E. Visser (Eline); T. Hoogenboezem (Theo); W.C.J. Hop (Wim); L.N.A. van Adrichem (Léon); F. Weber (Frank); H.A. Moll (Henriëtte); B. Broekman (Berth); M.Y. Berger (Marjolein); T. van Rijsoort-Vos (Tineke); A.F. van Belkum (Alex); M. Schutten (Martin); S.D. Pas (Suzan); A.D.M.E. Osterhaus (Albert); N.G. Hartwig (Nico); C. Vink (Cornelis); A.M.C. van Rossum (Annemarie)

    2013-01-01

    textabstractBackground:Mycoplasma pneumoniae is thought to be a common cause of respiratory tract infections (RTIs) in children. The diagnosis of M. pneumoniae RTIs currently relies on serological methods and/or the detection of bacterial DNA in the upper respiratory tract (URT). It is conceivable,

  19. Macrolide resistance determination and molecular typing of mycoplasma pneumoniae in respiratory specimens collected between 1997 and 2008 in The Netherlands.

    NARCIS (Netherlands)

    Spuesens, E.B.M.; Meijer, A.; Bierschenk, D.; Hoogenboezem, T.; Donker, G.A.; Hartwig, N.G.; Koopmans, M.P.G.; Vink, C.; Rossum, A.M.C. van

    2012-01-01

    An important role in the treatment regimens for Mycoplasma pneumoniae infections is played by macrolide (ML) antibiotics. In the past few years, however, a steady increase has been detected in the worldwide prevalence of ML-resistant (MLr) M. pneumoniae strains. It is obvious that this increase nece

  20. Increased rates of intensive care unit admission in patients with Mycoplasma pneumoniae: a retrospective study.

    Science.gov (United States)

    Khoury, T; Sviri, S; Rmeileh, A A; Nubani, A; Abutbul, A; Hoss, S; van Heerden, P V; Bayya, A E; Hidalgo-Grass, C; Moses, A E; Nir-Paz, R

    2016-08-01

    Mycoplasma pneumoniae is a leading cause of respiratory disease. In the Intensive Care Unit (ICU) setting M. pneumoniae is not considered a common pathogen. In 2010-13 an epidemic of M. pneumoniae-associated infections was reported and we observed an increase of M. pneumoniae patients admitted to ICU. We analysed the cohort of all M. pneumoniae-positive patients' admissions during 2007 to 2012 at the Hadassah-Hebrew University Medical Centre (a 1100-bed tertiary medical centre). Mycoplasma pneumoniae diagnosis was made routinely using PCR on throat swabs and other respiratory samples. Clinical parameters were retrospectively extracted. We identified 416 M. pneumoniae-infected patients; of which 68 (16.3%) were admitted to ICU. Of these, 48% (173/416) were paediatric patients with ICU admission rate of 4.6% (8/173). In the 19- to 65-year age group ICU admission rate rose to 18% (32/171), and to 38.8% (28/72) for patients older than 65 years. The mean APACHE II score on ICU admission was 20, with a median ICU stay of 7 days, and median hospital stay of 11.5 days. Of the ICU-admitted patients, 54.4% (37/68) were mechanically ventilated upon ICU admission. In 38.2% (26/68), additional pathogens were identified mostly later as secondary pathogens. A concomitant cardiac manifestation occurred in up to 36.8% (25/68) of patients. The in-hospital mortality was 29.4% (20/68) and correlated with APACHE II score. Contrary to previous reports, a substantial proportion (16.3%) of our M. pneumoniae-infected patients required ICU admission, especially in the adult population, with significant morbidity and mortality.

  1. Surveillance of Mycoplasma pneumoniae infection among children in Beijing from 2007 to 2012

    Institute of Scientific and Technical Information of China (English)

    Zhao Hanqing; Li Shaoli; Cao Ling; Yuan Yi; Xue Guanhua; Feng Yanling; Yan Chao

    2014-01-01

    Background Mycoplasma pneumonia (M.pneumoniae) is one of the key pathogens of community-acquired pneumonia.A global pandemic of M.pneumoniae has occurred since 2010.The aim of this study was to survey the prevalence of M.pneumoniae in children in Beijing from 2007-2012.Methods A total of 3 073 clinical specimens were obtained from pediatric patients with respiratory tract infections from January 2007 to December 2012,and examined by nested polymerase chain reaction.PCR products were visualized by 2% agarose gel electrophoresis,positive products sequenced,and compared with reference sequences in GenBank.Macrolide resistance-associated mutations were also detected for some positive samples.Results Of the 3 073 specimens,588 (19.13%) were positive for M.pneumoniae,12.4% of which were accompanied by viral infections.Positive rates for M.pneumoniae were highest in 2007 and 2012,showing a significant difference when compared with other years.Infections tended to occur in autumn and winter and positive rates were significantly higher for children aged 3-16.The rate of macrolide resistance-associated mutations was 90.7%,and the predominant mutation was an A→G transition (89.92%) at position 2063 in domain V of the 23S rRNA gene.Conclusions M.pneumoniae outbreaks occurred in 2007 and 2012 in pediatric patients in Beijing,which is consistent with the global prevalence of M.pneumoniae.M.pneumoniae can cause multi-system infections in children,and may be accompanied with viral infections.We determined that school-age children are more susceptible to this disease,particularly in autumn and winter.Gene mutations associated with macrolide resistance were very common in M.pneumoniae-positive specimens during this period in Beijing.

  2. Ateroesclerosis asociada a infección por chlamydophila pneumoniae: interacción entre el ser humano y una bacteria

    Directory of Open Access Journals (Sweden)

    Alvaro Gudiño-Gomezjurado

    2015-04-01

    Full Text Available La ateroesclerosis y sus complicaciones constituyen una de las mayores causas de morbi-mortalidad a nivel mundial. Sin embargo, solo la mitad de los casos se justifica por los factores de riesgo tradicionales. No obstante, desde hace casi cinco décadas se ha intentado encontrar causas no tradicionales asociadas a la enfermedad ateroesclerótica. Varios agentes infecciosos han emergido como posibles candidatos centralizándose la investigación en Chlamydophila pneumoniae por su capacidad de infectar las células endoteliales durante el proceso ateroesclerótico. A lo largo del tiempo, múltiples estudios han tratado de probar la causalidad de C. pneumoniae en el proceso ateroesclerótico y sus complicaciones. A pesar de esto, hasta la fecha las conclusiones son ambiguas y de poca relevancia para la práctica clínica diaria.

  3. 儿童肺炎支原体感染的临床研究进展%Clinical Research Progress of Mycoplasma Pneumoniae Infection in Children

    Institute of Scientific and Technical Information of China (English)

    王松; 何玲

    2015-01-01

    肺炎支原体(mycoplasma pneumoniae,MP)是儿童呼吸道感染的最常见病原之一.相关研究显示,近年来小儿肺炎支原体肺炎(mycoplasma pneumoniae pneumonia,MPP)的发病率不仅有上升趋势,而且重症支原体肺炎(severe mycoplasma pneumoniae pneumonia,SMPP)和难治性支原体肺炎(refractory mycoplasma pneumoniae pneumonia,RMPP)日趋增多.临床上应对小儿支原体感染的诊治十分棘手,尤其对其早期有效检测方法的缺乏和对大环内酯类抗生素耐药等问题不容忽视.

  4. Pneumonia adquirida na comunidade e derrame pleural parapneumônico relacionados a Mycoplasma pneumoniae em crianças e adolescentes Mycoplasma pneumoniae-related community-acquired pneumonia and parapneumonic pleural effusion in children and adolescents

    Directory of Open Access Journals (Sweden)

    Letícia Alves Vervloet

    2012-04-01

    Full Text Available OBJETIVO: Determinar a prevalência e as características da pneumonia adquirida na comunidade (PAC e derrames pleurais parapneumônicos (DPP relacionados a Mycoplasma pneumoniae em um grupo de crianças e adolescentes. MÉTODOS: Estudo observacional retrospectivo com 121 pacientes hospitalizados com PAC e DPP em um hospital de referência terciária, entre 2000 e 2008, divididos em seis grupos (G1 a G6 segundo o agente etiológico: M. pneumoniae com ou sem coinfecção, em 44 pacientes; outros agentes que não M. pneumoniae, em 77; M. pneumoniae sem coinfecção, em 34; Streptococcus pneumoniae, em 36; Staphylococcus aureus, em 31; e coinfecção M. pneumoniae/S. pneumoniae, em 9, respectivamente. RESULTADOS: Na comparação entre os grupos, G1 apresentou frequências maiores em gênero feminino, tosse seca, uso prévio de beta-lactâmicos e na duração dos sintomas até a admissão, assim como menor uso de assistência ventilatória e de drenagem torácica que G2, enquanto G3 teve maiores frequências em uso prévio de beta-lactâmicos e tosse seca, maior duração dos sintomas antes da admissão e menor frequência de uso de drenos torácicos que G4 e G5, ao passo que G3 teve média de idade maior e menor frequência de náuseas/vômitos que G4, assim como menor uso de assistência ventilatória que G5. A coinfecção M. pneumoniae/S. pneumoniae aumentou a duração dos sintomas até a admissão. CONCLUSÕES: Nesta amostra, a prevalência de PAC e DPP por M. pneumoniae foi de 12,75%. Embora a doença apresentasse quadros mais leves que aquela por outros organismos, a evolução foi mais prolongada. Nossos dados sugerem a necessidade de uma maior diligência na investigação de M. pneumoniae em crianças e adolescentes com PAC e DPP em nosso meio.OBJECTIVE: To determine the prevalence and the characteristics of Mycoplasma pneumoniae-related community-acquired pneumonia (CAP and parapneumonic pleural effusion (PPE in children and adolescents

  5. Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA

    Directory of Open Access Journals (Sweden)

    Birkelund Svend

    2004-02-01

    Full Text Available Abstract Background Serology is often used for the diagnosis of Mycoplasma pneumoniae. It is important to identify specific antigens that can distinguish between the presence or absence of antibodies against M. pneumoniae. The two proteins, P116 and P1, are found to be immunogenic. By using these in ELISA it is possible to identify an immune response against M. pneumoniae in serum samples. Results A recombinant protein derived from the P116 protein and one from the P1 protein were used in two ELISA tests, rP116-ELISA and rP1-ELISA. Human serum samples from patients with atypical pneumonia were tested and compared to the results of the complement fixation test. There was a good agreement between the two tests but the rP1-ELISA showed the best discrimination between positive and negative samples. Conclusion Two ELISA tests based on recombinant proteins have been analysed and compared to the complement fixation test results. The two ELISA tests were found suitable for use in serodiagnostics of M. pneumoniae infections. The use of specific antigens eliminates the risk of cross reaction to an immune response against other bacteria.

  6. Evaluation and optimization of a commercial enzyme linked immunosorbent assay for detection of Chlamydophila pneumoniae IgA antibodies

    Directory of Open Access Journals (Sweden)

    Gargouri Jalel

    2008-07-01

    Full Text Available Abstract Background Serologic diagnosis of Chlamydophila pneumoniae (Cpn infection routinely involves assays for the presence of IgG and IgM antibodies to Cpn. Although IgA antibodies to Cpn have been found to be of interest in the diagnosis of chronic infections, their significance in serological diagnosis remains unclear. The microimmunofluorescence (MIF test is the current method for the measurement of Cpn antibodies. While commercial enzyme linked immunosorbent assays (ELISA have been developed, they have not been fully validated. We therefore evaluated and optimized a commercial ELISA kit, the SeroCP IgA test, for the detection of Cpn IgA antibodies. Methods Serum samples from 94 patients with anti-Cpn IgG titers ≥ 256 (study group and from 100 healthy blood donors (control group were tested for the presence of IgA antibodies to Cpn, using our in-house MIF test and the SeroCP IgA test. Two graph receiver operating characteristic (TG-ROC curves were created to optimize the cut off given by the manufacturer. Results The MIF and SeroCP IgA tests detected Cpn IgA antibodies in 72% and 89%, respectively, of sera from the study group, and in 9% and 35%, respectively, of sera from the control group. Using the MIF test as the reference method and the cut-off value of the ELISA test specified by the manufacturer for seropositivity and negativity, the two tests correlated in 76% of the samples, with an agreement of Ƙ = 0.54. When we applied the optimized cut-off value using TG-ROC analysis, 1.65, we observed better concordance (86% and agreement (0.72 between the MIF and SeroCP IgA tests. Conclusion Use of TG-ROC analysis may help standardize and optimize ELISAs, which are simpler, more objective and less time consuming than the MIF test. Standardization and optimization of commercial ELISA kits may result in better performance.

  7. A Rare Case of Cavitary Lesion of the Lung Caused by Mycoplasma pneumoniae in an Immunocompetent Patient

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    Muhammad Kashif

    2017-01-01

    Full Text Available Mycoplasma pneumoniae is an atypical bacterium that most commonly causes upper respiratory tract infections, but it can also cause pneumonia, referred to as “walking pneumonia.” Although cavitary lesions are present in a wide variety of infectious and noninfectious processes, those attributable to M. pneumoniae are extremely uncommon; thus, to date, epidemiological studies are lacking. Here, we present a rare case of a 20-year-old male, referred to us from a psychiatric facility for evaluation of a cough, who was found to have a cavitary lesion in the right upper lobe. An extensive workup for cavitary lesion was negative, but his mycoplasma IgM level was high. A computed tomography (CT of the chest confirmed the presence of a cavitary lesion. After treatment with levofloxacin antibiotics, a follow-up CT showed complete resolution of the lesion. Our case is a rare presentation of mycoplasma pneumonia as a cavitary lesion in a patient without any known risk factors predisposing to mycoplasma infection. Early recognition and treatment with an appropriate antibiotic may lead to complete resolution of the cavitary lesion.

  8. The relationship of Chlamydophila pneumoniae with schizophrenia: The role of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in this relationship.

    Science.gov (United States)

    Kalayci, Fatma; Ozdemir, Armagan; Saribas, Suat; Yuksel, Pelin; Ergin, Sevgi; Kuskucu, Ali Mert; Poyraz, Cana Aksoy; Balcioglu, Ibrahim; Alpay, Nihat; Kurt, Aykut; Sezgin, Zeynep; Kocak, Banu Tufan; Icel, Rana Sucu; Can, Gunay; Tokman, Hrisi Bahar; Kocazeybek, Bekir

    Several pathogens have been suspected of playing a role in the pathogenesis of schizophrenia. Chronic inflammation has been proposed to occur as a result of persistent infection caused by Chlamydophila pneumoniae cells that reside in brain endothelial cells for many years. It was recently hypothesized that brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) may play prominent roles in the development of schizophrenia. NT-3 and BDNF levels have been suggested to change in response to various manifestations of infection. Therefore, we aimed to elucidate the roles of BDNF and NT3 in the schizophrenia-C. pneumoniae infection relationship. RT-PCR, immunofluorescence and ELISA methods were used. Fifty patients suffering from schizophrenia and 35 healthy individuals were included as the patient group (PG) and the healthy control group (HCG), respectively. We detected persistent infection in 14 of the 50 individuals in the PG and in 1 of the 35 individuals in the HCG. A significant difference was found between the two groups (p0.05). C. pneumoniae DNA was not detected in any group. A significant difference in NT-3 levels was observed between the groups, with very low levels in the PG (p0.05). In conclusion, we suggest that NT-3 levels during persistent C. pneumoniae infection may play a role in this relationship. Copyright © 2016 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  9. Chlamydophila pneumoniae HflX belongs to an uncharacterized family of conserved GTPases and associates with the Escherichia coli 50S large ribosomal subunit.

    Science.gov (United States)

    Polkinghorne, Adam; Ziegler, Urs; González-Hernández, Yanela; Pospischil, Andreas; Timms, Peter; Vaughan, Lloyd

    2008-11-01

    Predicted members of the HflX subfamily of phosphate-binding-loop guanosine triphosphatases (GTPases) are widely distributed in the bacterial kingdom but remain virtually uncharacterized. In an attempt to understand mechanisms used for regulation of growth and development in the chlamydiae, obligate intracellular and developmentally complex bacteria, we have begun investigations into chlamydial GTPases; we report here what appears to be the first analysis of a HflX family GTPase using a predicted homologue from Chlamydophila pneumoniae. In agreement with phylogenetic predictions for members of this GTPase family, purified recombinant Cp. pneumoniae HflX was specific for guanine nucleotides and exhibited a slow intrinsic GTPase activity when incubated with [gamma-(32)P]GTP. Using HflX-specific monoclonal antibodies, HflX could be detected by Western blotting and high-resolution confocal microscopy throughout the vegetative growth cycle of Cp. pneumoniae and, at early time points, appeared to partly localize to the membrane. Ectopic expression of Cp. pneumoniae HflX in Escherichia coli revealed co-sedimentation of HflX with the E. coli 50S large ribosomal subunit. The results of this work open up some intriguing possibilities for the role of GTPases belonging to this previously uncharacterized family of bacterial GTPases. Ribosome association is a feature shared by other important conserved GTPase families and more detailed investigations will be required to delineate the role of HflX in bacterial ribosome function.

  10. A novel inhibitor of Chlamydophila pneumoniae protein kinase D (PknD inhibits phosphorylation of CdsD and suppresses bacterial replication

    Directory of Open Access Journals (Sweden)

    Bulir David C

    2009-10-01

    Full Text Available Abstract Background We have shown previously that Chlamydophila pneumoniae contains a dual-specific Ser/Thr protein kinase that phosphorylates CdsD, a structural component of the type III secretion apparatus. To further study the role of PknD in growth and development we sought to identify a PknD inhibitor to determine whether PknD activity is required for replication. Results Using an in vitro kinase assay we screened 80 known eukaryotic protein kinase inhibitors for activity against PknD and identified a 3'-pyridyl oxindole compound that inhibited PknD autophosphorylation and phosphorylation of CdsD. The PknD inhibitor significantly retarded the growth rate of C. pneumoniae as evidenced by the presence of very small inclusions with a reduced number of bacteria as seen by electron microscopy. These inclusions contained the normal replicative forms including elementary bodies (EB, intermediate bodies (IB and reticulate bodies (RB, but lacked persistent bodies (PB, indicating that induction of persistence was not the cause of reduced chlamydial growth. Blind passage of C. pneumoniae grown in the presence of this PknD inhibitor for 72 or 84 hr failed to produce inclusions, suggesting this compound blocks an essential step in the production of infectious chlamydial EB. The compound was not toxic to HeLa cells, did not block activation of the MEK/ERK pathway required for chlamydial invasion and did not block intracellular replication of either Chlamydia trachomatis serovar D or Salmonella enterica sv. Typhimurium suggesting that the inhibitory effect of the compound is specific for C. pneumoniae. Conclusion We have identified a 3'-pyridyl oxindole compound that inhibits the in vitro kinase activity of C. pneumoniae PknD and inhibits the growth and production of infectious C. pneumoniae progeny in HeLa cells. Together, these results suggest that PknD may play a key role in the developmental cycle of C. pneumoniae.

  11. Detection and characterization of Mycoplasma pneumoniae during an outbreak of respiratory illness at a university.

    Science.gov (United States)

    Waller, Jessica L; Diaz, Maureen H; Petrone, Brianna L; Benitez, Alvaro J; Wolff, Bernard J; Edison, Laura; Tobin-D'Angelo, Melissa; Moore, Ashley; Martyn, Audrey; Dishman, Hope; Drenzek, Cherie L; Turner, Kim; Hicks, Lauri A; Winchell, Jonas M

    2014-03-01

    An outbreak at a university in Georgia was identified after 83 cases of probable pneumonia were reported among students. Respiratory specimens were obtained from 21 students for the outbreak investigation. The TaqMan array card (TAC), a quantitative PCR (qPCR)-based multipathogen detection technology, was used to initially identify Mycoplasma pneumoniae as the causative agent in this outbreak. TAC demonstrated 100% diagnostic specificity and sensitivity compared to those of the multiplex qPCR assay for this agent. All M. pneumoniae specimens (n=12) and isolates (n=10) were found through genetic analysis to be susceptible to macrolide antibiotics. The strain diversity of M. pneumoniae associated with this outbreak setting was identified using a variety of molecular typing procedures, resulting in two P1 genotypes (types 1 [60%] and 2 [40%]) and seven different multilocus variable-number tandem-repeat analysis (MLVA) profiles. Continued molecular typing of this organism, particularly during outbreaks, may enhance the current understanding of the epidemiology of M. pneumoniae and may ultimately lead to a more effective public health response.

  12. Comparison of Mycoplasma pneumoniae Genome Sequences from Strains Isolated from Symptomatic and Asymptomatic Patients

    Science.gov (United States)

    Spuesens, Emiel B. M.; Brouwer, Rutger W. W.; Mol, Kristin H. J. M.; Hoogenboezem, Theo; Kockx, Christel E. M.; Jansen, Ruud; Van IJcken, Wilfred F. J.; Van Rossum, Annemarie M. C.; Vink, Cornelis

    2016-01-01

    Mycoplasma pneumoniae is a common cause of respiratory tract infections (RTIs) in children. We recently demonstrated that this bacterium can be carried asymptomatically in the respiratory tract of children. To identify potential genetic differences between M. pneumoniae strains that are carried asymptomatically and those that cause symptomatic infections, we performed whole-genome sequence analysis of 20 M. pneumoniae strains. The analyzed strains included 3 reference strains, 3 strains isolated from asymptomatic children, 13 strains isolated from clinically well-defined patients suffering from an upper (n = 4) or lower (n = 9) RTI, and one strain isolated from a follow-up patient who recently recovered from an RTI. The obtained sequences were each compared to the sequences of the reference strains. To find differences between strains isolated from asymptomatic and symptomatic individuals, a variant comparison was performed between the different groups of strains. Irrespective of the group (asymptomatic vs. symptomatic) from which the strains originated, subtype 1 and subtype 2 strains formed separate clusters. We could not identify a specific genotype associated with M. pneumoniae virulence. However, we found marked genetic differences between clinical isolates and the reference strains, which indicated that the latter strains may not be regarded as appropriate representatives of circulating M. pneumoniae strains. PMID:27833597

  13. Comparison of Mycoplasma pneumoniae genome sequences from strains isolated from symptomatic and asymptomatic patients

    Directory of Open Access Journals (Sweden)

    Emiel B.M. Spuesens

    2016-10-01

    Full Text Available Mycoplasma pneumoniae is a common cause of respiratory tract infections (RTI in children. We recently demonstrated that this bacterium can be carried asymptomatically in the respiratory tract of children. To identify potential genetic differences between M. pneumoniae strains that are carried asymptomatically and those that cause symptomatic infections, we performed whole-genome sequence analysis of 20 M. pneumoniae strains. The analyzed strains included 3 reference strains, 3 strains isolated from asymptomatic children, 13 strains isolated from clinically well-defined patients suffering from an upper (n=4 or lower (n=9 RTI, and one strain isolated from a follow-up patient who recently recovered from an RTI. The obtained sequences were each compared to the sequences of the reference strains. To find differences between strains isolated from asymptomatic and symptomatic individuals, a variant comparison was performed between the different groups of strains. Irrespective of the group (asymptomatic versus symptomatic from which the strains originated, subtype 1 and subtype 2 strains formed separate clusters. We could not identify a specific genotype associated with M. pneumoniae virulence. However, we found marked genetic differences between clinical isolates and the reference strains, which indicated that the latter strains may not be regarded as appropriate representatives of circulating M. pneumoniae strains.

  14. Chronic meningitis with intracranial hypertension and bilateral neuroretinitis following Mycoplasma pneumoniae infection.

    Science.gov (United States)

    Karampatsas, Konstantinos; Patel, Himanshu; Basheer, Sheikh N; Prendergast, Andrew J

    2014-12-23

    A previously well 12-year-old boy presented with a 2-week history of headache, nausea, vomiting and left-sided weakness. He subsequently developed meningism, right abducens nerve palsy, persistent papilloedema and reduced visual acuity in association with a bilateral macular star, consistent with neuroretinitis. Cerebrospinal fluid (CSF) examination indicated chronic meningitis and serological testing confirmed recent Mycoplasma pneumoniae infection, although PCR in CSF was negative. He was treated for aseptic meningitis with ceftriaxone, aciclovir, azithromycin and acetazolamide for intracranial hypertension, with gradual improvement in clinical condition and visual acuity over several weeks. This is the first report of M. pneumoniae chronic meningitis further complicated with bilateral neuroretinitis and intracranial hypertension. Evidence of central nervous system inflammation in the absence of direct infection suggests an immune-mediated pathophysiology. Although the use of macrolides with antibiotic and immunomodulatory activity might be beneficial, it was not possible to ascertain whether it influenced clinical recovery in this case.

  15. Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia.

    Science.gov (United States)

    Dassanayake, Rohana P; Shanthalingam, Sudarvili; Herndon, Caroline N; Subramaniam, Renuka; Lawrence, Paulraj K; Bavananthasivam, Jegarubee; Cassirer, E Frances; Haldorson, Gary J; Foreyt, William J; Rurangirwa, Fred R; Knowles, Donald P; Besser, Thomas E; Srikumaran, Subramaniam

    2010-10-26

    Mycoplasma ovipneumoniae has been isolated from the lungs of pneumonic bighorn sheep (BHS). However experimental reproduction of fatal pneumonia in BHS with M. ovipneumoniae was not successful. Therefore the specific role, if any, of M. ovipneumoniae in BHS pneumonia is unclear. The objective of this study was to determine whether M. ovipneumoniae alone causes fatal pneumonia in BHS, or predisposes them to infection by Mannheimia haemolytica. We chose M. haemolytica for this study because of its isolation from pneumonic BHS, and its consistent ability to cause fatal pneumonia under experimental conditions. Since in vitro culture could attenuate virulence of M. ovipneumoniae, we used ceftiofur-treated lung homogenates from pneumonic BHS lambs or nasopharyngeal washings from M. ovipneumoniae-positive domestic sheep (DS) as the source of M. ovipneumoniae. Two adult BHS were inoculated intranasally with lung homogenates while two others received nasopharyngeal washings from DS. All BHS developed clinical signs of respiratory infection, but only one BHS died. The dead BHS had carried leukotoxin-positive M. haemolytica in the nasopharynx before the onset of this study. It is likely that M. ovipneumoniae colonization predisposed this BHS to fatal infection with the M. haemolytica already present in this animal. The remaining three BHS developed pneumonia and died 1-5 days following intranasal inoculation with M. haemolytica. On necropsy, lungs of all four BHS showed lesions characteristic of bronchopneumonia. M. haemolytica and M. ovipneumoniae were isolated from the lungs. These results suggest that M. ovipneumoniae alone may not cause fatal pneumonia in BHS, but can predispose them to fatal pneumonia due to M. haemolytica infection. Copyright © 2010 Elsevier B.V. All rights reserved.

  16. Mycoplasma ovipneumoniae - A Primary Cause of Severe Pneumonia Epizootics in the Norwegian Muskox (Ovibos moschatus) Population

    Science.gov (United States)

    Handeland, Kjell; Tengs, Torstein; Kokotovic, Branko; Vikøren, Turid; Ayling, Roger D.; Bergsjø, Bjarne; Sigurðardóttir, Ólöf G.; Bretten, Tord

    2014-01-01

    The Norwegian muskox (Ovibos moschatus) population lives on the high mountain plateau of Dovre and originates from animals introduced from Greenland. In the late summers of 2006 and 2012, severe outbreaks of pneumonia with mortality rates of 25-30% occurred. During the 2012 epidemic high quality samples from culled sick animals were obtained for microbiological and pathological examinations. High throughput sequencing (pyrosequencing) of pneumonic lung tissue revealed high concentrations of Mycoplasma ovipneumoniae in all six animals examined by this method and Pasteurella multocida subsp. multocida in four animals, whereas no virus sequences could be identified. Mycoplasma ovipneumoniae and P. multocida multocida were also isolated by culture. Using real time PCR on lung swabs, M. ovipneumoniae was detected in all of the 19 pneumonic lungs examined. Gross pathological examination revealed heavy consolidations primarily in the cranial parts of the lungs and it also identified one case of otitis media. Histologically, lung lesions were characterized as acute to subacute mixed exudative and moderately proliferative bronchoalveolar pneumonia. Immunohistochemical (IHC) examination revealed high load of M. ovipneumoniae antigens within lung lesions, with particularly intensive staining in the neutrophils. Similar IHC finding were observed in archived lung tissue blocks from animals examined during the 2006 epidemic. An M. ovipneumoniae specific ELISA was applied on bio-banked muskox sera from stray muskoxen killed in the period 2004–2013 and sick muskoxen culled, as well as sera from wild reindeer (Rangifer tarandus tarandus) on Dovre and muskoxen from Greenland. Serology and mycoplasma culturing was also carried out on sheep that had been on pasture in the muskox area during the outbreak in 2012. Our findings indicated separate introductions of M. ovipneumoniae infection in 2006 and 2012 from infected co-grazing sheep. Salt licks shared by the two species were a

  17. Immune haemolytic anaemia associated with ampicillin dependent warm antibodies and high titre cold agglutinins in a patient with Mycoplasma pneumonia

    DEFF Research Database (Denmark)

    Mickley, H; Sørensen, P G

    1984-01-01

    A case of severe immune haemolytic anaemia in a 54-year-old man suffering from Mycoplasma pneumonia is presented. A strongly positive direct Coombs test with erythrocyte bound IgG, C3d and C4 was demonstrated during the haemolytic process. Further, serologic investigations revealed ampicillin...

  18. Antibiotic susceptibility in relation to genotype of Streptococcus pneumoniae, Haemophilus influenzae, and Mycoplasma pneumoniae responsible for community-acquired pneumonia in children.

    Science.gov (United States)

    Morozumi, Miyuki; Chiba, Naoko; Okada, Takafumi; Sakata, Hiroshi; Matsubara, Keita; Iwata, Satoshi; Ubukata, Kimiko

    2013-06-01

    Streptococcus pneumoniae, Haemophilus influenzae, and Mycoplasma pneumoniae are the main pathogens causing community-acquired pneumonia (CAP). We identified S. pneumoniae (n = 241), H. influenzae (n = 123), and M. pneumoniae (n = 54) as causative pathogens from clinical findings and blood tests from pediatric CAP patients (n = 903) between April 2008 and April 2009. Identification of genes mediating antimicrobial resistance by real-time PCR was performed for all isolates of these three pathogens, as was antibiotic susceptibility testing using an agar dilution method or broth microdilution method. The genotypic (g) resistance rate was 47.7 % for penicillin-resistant S. pneumoniae (gPRSP) possessing abnormal pbp1a, pbp2x, and pbp2b genes, 62.6 % for β-lactamase-nonproducing, ampicillin-resistant (gBLNAR) H. influenzae possessing the amino acid substitutions Ser385Thr and Asn526Lys, and 44.4 % for macrolide-resistant M. pneumoniae (gMRMP) possessing a mutation of A2063G, A2064G, or C2617A. Serotype 6B (20.3 %) predominated in S. pneumoniae, followed by 19F (15.4 %), 14 (14.5 %), 23F (12.0 %), 19A (6.2 %), and 6C (5.4 %). Coverage for the isolates by heptavalent pneumococcal conjugate vaccine (PCV7) and PCV13, respectively, was calculated as 68.5 and 80.9 %. A small number of H. influenzae were identified as type b (6.5 %), type e (0.8 %), or type f (0.8 %); all others were nontypeable. Proper use of antibiotics based on information about resistance in CAP pathogens is required to control rapid increases in resistance. Epidemiological surveillance of pediatric patients also is needed to assess the effectiveness of PCV7 and Hib vaccines after their introduction in Japan.

  19. Macrolide resistance determination and molecular typing of Mycoplasma pneumoniae by pyrosequencing.

    Science.gov (United States)

    Spuesens, Emiel B M; Hoogenboezem, Theo; Sluijter, Marcel; Hartwig, Nico G; van Rossum, Annemarie M C; Vink, Cornelis

    2010-09-01

    The first choice antibiotics for treatment of Mycoplasma pneumoniae infections are macrolides. Several recent studies, however, have indicated that the prevalence of macrolide (ML)-resistance, which is determined by mutations in the bacterial 23S rRNA, is increasing among M. pneumoniae isolates. Consequently, it is imperative that ML-resistance in M. pneumoniae is rapidly detected to allow appropriate and timely treatment of patients. We therefore set out to determine the utility of pyrosequencing as a convenient technique to assess ML-resistance. In addition, we studied whether pyrosequencing could be useful for molecular typing of M. pneumoniae isolates. To this end, a total of four separate pyrosequencing assays were developed. These assays were designed such as to determine a short genomic sequence from four different sites, i.e. two locations within the 23S rRNA gene, one within the MPN141 (or P1) gene and one within the MPN528a gene. While the 23S rRNA regions were employed to determine ML-resistance, the latter two were used for molecular typing. The pyrosequencing assays were performed on a collection of 108 M. pneumoniae isolates. The ML-resistant isolates within the collection (n=4) were readily identified by pyrosequencing. Moreover, each strain was correctly typed as either a subtype 1 or subtype 2 strain by both the MPN141 and MPN528a pyrosequencing test. Interestingly, two recent isolates from our collection, which were identified as subtype 2 strains by the pyrosequencing assays, were found to carry novel variants of the MPN141 gene, having rearrangements in each of the two repetitive elements (RepMP4 and RepMP2/3) within the gene. In conclusion, pyrosequencing is a convenient technique for ML-resistance determination as well as molecular typing of M. pneumoniae isolates.

  20. Exposure of bighorn sheep to domestic goats colonized with Mycoplasma ovipneumoniae induces sub-lethal pneumonia.

    Science.gov (United States)

    Besser, Thomas E; Cassirer, E Frances; Potter, Kathleen A; Foreyt, William J

    2017-01-01

    Bronchopneumonia is a population limiting disease of bighorn sheep (Ovis canadensis) that has been associated with contact with domestic Caprinae. The disease is polymicrobial but is initiated by Mycoplasma ovipneumoniae, which is commonly carried by both domestic sheep (O. aries) and goats (Capra aegagrus hircus). However, while previous bighorn sheep comingling studies with domestic sheep have resulted in nearly 100% pneumonia mortality, only sporadic occurrence of fatal pneumonia was reported from previous comingling studies with domestic goats. Here, we evaluated the ability of domestic goats of defined M. ovipneumoniae carriage status to induce pneumonia in comingled bighorn sheep. In experiment 1, three bighorn sheep naïve to M. ovipneumoniae developed non-fatal respiratory disease (coughing, nasal discharge) following comingling with three naturally M. ovipneumoniae-colonized domestic goats. Gross and histological lesions of pneumonia, limited to small areas on the ventral and lateral edges of the anterior and middle lung lobes, were observed at necropsies conducted at the end of the experiment. A control group of three bighorn sheep from the same source housed in isolation during experiment 1 remained free of observed respiratory disease. In experiment 2, three bighorn sheep remained free of observed respiratory disease while comingled with three M. ovipneumoniae-free domestic goats. In experiment 3, introduction of a domestic goat-origin strain of M. ovipneumoniae to the same comingled goats and bighorn sheep used in experiment 2 resulted in clinical signs of respiratory disease (coughing, nasal discharge) in both host species. At the end of experiment 3, gross and histological evidence of pneumonia similar to that observed in experiment 1 bighorn sheep was observed in both affected bighorn sheep and domestic goats. M. ovipneumoniae strains carried by domestic goats were transmitted to comingled bighorn sheep, triggering development of pneumonia. However

  1. Detection of Mycoplasma pneumoniae in simulated and true clinical throat swab specimens by nanorod array-surface-enhanced Raman spectroscopy.

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    Suzanne L Hennigan

    Full Text Available The prokaryote Mycoplasma pneumoniae is a major cause of respiratory disease in humans, accounting for 20% of all community-acquired pneumonia and the leading cause of pneumonia in older children and young adults. The limitations of existing options for mycoplasma diagnosis highlight a critical need for a new detection platform with high sensitivity, specificity, and expediency. Here we evaluated silver nanorod arrays (NA as a biosensing platform for detection and differentiation of M. pneumoniae in culture and in spiked and true clinical throat swab samples by surface-enhanced Raman spectroscopy (SERS. Three M. pneumoniae strains were reproducibly differentiated by NA-SERS with 95%-100% specificity and 94-100% sensitivity, and with a lower detection limit exceeding standard PCR. Analysis of throat swab samples spiked with M. pneumoniae yielded detection in a complex, clinically relevant background with >90% accuracy and high sensitivity. In addition, NA-SERS correctly classified with >97% accuracy, ten true clinical throat swab samples previously established by real-time PCR and culture to be positive or negative for M. pneumoniae. Our findings suggest that the unique biochemical specificity of Raman spectroscopy, combined with reproducible spectral enhancement by silver NA, holds great promise as a superior platform for rapid and sensitive detection and identification of M. pneumoniae, with potential for point-of-care application.

  2. Classification of extrapulmonary manifestations due to Mycoplasma pneumoniae infection on the basis of possible pathogenesis

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    Mitsuo eNarita

    2016-01-01

    Full Text Available The list of extrapulmonary manifestations due to Mycoplasma pneumoniae infection can be classified according to the following three possible mechanisms derived from the established biological activity of M. pneumoniae; 1 a direct type in which the bacterium is present at the site of inflammation and local inflammatory cytokines induced by the bacterium play an important role 2 an indirect type in which the bacterium is not present at the site of inflammation and immune modulations, such as autoimmunity or formation of immune complexes, play an important role and 3 a vascular occlusion type in which obstruction of blood flow induced either directly or indirectly by the bacterium plays an important role. Recent studies concerning extrapulmonary manifestations have prompted the author to upgrade the list, including cardiac and aortic thrombi as cardiovascular manifestations; erythema nodosum, cutaneous leukocytoclastic vasculitis, and subcorneal pustular dermatosis as dermatological manifestations; acute cerebellar ataxia, opsoclonus-myoclonus syndrome, and thalamic necrosis as neurological manifestations; pulmonary embolism as a respiratory system manifestation; and renal artery embolism as a urogenital tract manifestation. Continuing nosological confusion on M. pneumoniae–induced mucositis (without skin lesions, which may be called M. pneumoniae-associated mucositis or M. pneumoniae-induced rash and mucositis separately from Stevens-Johnson syndrome, is argued in the dermatological manifestations. Serological methods are recommended for diagnosis because pneumonia or respiratory symptoms are often minimal or even absent in extrapulmonary manifestations due to M. pneumoniae infection. Concomitant use of immunomodulators, such as corticosteroids or immunoglobulins with antibiotics effective against M. pneumoniae, can be considered as treatment modalities for most severe cases, such as encephalitis. Further studies would be necessary to construct

  3. 难治性肺炎支原体肺炎的发病机制%Mechanisms of refractory Mycoplasma pneumoniae pneumonia in children

    Institute of Scientific and Technical Information of China (English)

    刘金荣; 赵顺英

    2013-01-01

    大多数肺炎支原体肺炎(MPP)经大环内酯类抗生素治疗后病情控制,但近年发现难治性肺炎支原体肺炎(RMPP)较前增多(其中一部分为重症肺炎支原体肺炎,SMPP),常导致严重肺内外并发症,如坏死性肺炎等,并且易遗留闭塞性细/支气管炎、肺不张以及支气管扩张等后遗症。目前认为MPP是由于肺炎支原体(MP)直接侵犯肺部和支气管组织以及MP激发机体的过度炎症反应两个主要原因造成,而RMPP尚与MP载量、MP耐药、气道黏液高分泌、高凝状态、混合细菌或病毒感染和社区获得性呼吸窘迫综合征毒素产生有关。探索RMPP的发病机制,以探索有效干预方法,阻止后遗症的发生。%Most of Mycoplasma pneumoniae pneumonia (MPP) will be controlled after being treated with macrolide antibiotics. But in recent years refractory Mycoplasma pneumoniae pneumonia (RMPP), including severe mycoplasma pneumoniae pneumonia, are increased. RMPP often causes many severe complications and sequelae such as necrotizing pneumonia, bronchiolitis obliterans, bronchitis obliterans, atelectasis and bronchiectasis. Two main mechanisms of MPP are proposed: damages to airway directly by mycoplasma pneumonia (MP), and inflammatory reaction caused by mycoplasma pneumoniae (MP). RMPP is also associated with many other factors such as MP genotype and load, macrolide-resistant MP, airway mucus hypersecretion, hypercoagulability, combined infection with bacteria or viruses, and community acquired respiratory distress syndrome toxin (CARDS Tx). The exploration of mechanisms of RMPP helps us acquire effective treatment and prevent sequelae.

  4. Severe Hemolytic Anemia Associated with Mild Pneumonia Caused by Mycoplasma pneumonia

    Directory of Open Access Journals (Sweden)

    Zafer Kurugol

    2012-01-01

    Full Text Available We report a case of M. pneumoniae infection presenting with severe hemolytic anemia in a 4-year-old girl, with a ten-day history of paleness, weakness, and nonproductive cough. She was very pale and tachycardic. However, she was not tachypneic. Chest examination showed normal breath sounds. No rhoncus or whistling was heard. As the erythrocyte sedimentation rate was excessively elevated, the differential diagnosis primarily comprised hematological malignancies. Direct Coombs' test was positive. Diagnosis of M. pneumoniae infection was confirmed by elevated levels of M. pneumoniae IgG and IgM antibodies and a chest X-ray suggestive of atypical pneumonia. The patient was treated with clarithromycin and packed red cell transfusion and showed a favorable recovery within ten days after admission. In conclusion, this case demonstrates that severe hemolytic anemia caused by M. pneumoniae is not always associated with severe pulmonary involvement, even when the respiratory infection is very mild, M. pneumoniae may be the cause of severe anemia.

  5. Mycoplasma pneumoniae large DNA repetitive elements RepMP1 show type specific organization among strains.

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    Oxana Musatovova

    Full Text Available Mycoplasma pneumoniae is the smallest self-replicating bacterium with a streamlined genome of 0.81 Mb. Complete genome analysis revealed the presence of multiple copies of four large repetitive elements (designated RepMP1, RepMP2/3, RepMP4 and RepMP5 that are implicated in creating sequence variations among individual strains. Recently, we described RepMP1-associated sequence variations between reference strain M129 and clinical isolate S1 that involved three RepMP1-genes (i.e. mpn130, mpn137 and mpn138. Using PCR and sequencing we analyze 28 additional M. pneumoniae strains and demonstrate the existence of S1-like sequence variants in nine strains and M129-like variants in the remaining nineteen strains. We propose a series of recombination steps that facilitates transition from M129- to S1-like sequence variants. Next we examined the remaining RepMP1-genes and observed no other rearrangements related to the repeat element. The only other detected difference was varying numbers of the 21-nucleotide tandem repeats within mpn127, mpn137, mpn501 and mpn524. Furthermore, typing of strains through analysis of large RepMPs localized within the adhesin P1 operon revealed that sequence divergence involving RepMP1-genes mpn130, mpn137 and mpn138 is strictly type-specific. Once more our analysis confirmed existence of two highly conserved groups of M. pneumoniae strains.

  6. Epizootic pneumonia of bighorn sheep following experimental exposure to Mycoplasma ovipneumoniae.

    Science.gov (United States)

    Besser, Thomas E; Cassirer, E Frances; Potter, Kathleen A; Lahmers, Kevin; Oaks, J Lindsay; Shanthalingam, Sudarvili; Srikumaran, Subramaniam; Foreyt, William J

    2014-01-01

    Bronchopneumonia is a population limiting disease of bighorn sheep (Ovis canadensis). The cause of this disease has been a subject of debate. Leukotoxin expressing Mannheimia haemolytica and Bibersteinia trehalosi produce acute pneumonia after experimental challenge but are infrequently isolated from animals in natural outbreaks. Mycoplasma ovipneumoniae, epidemiologically implicated in naturally occurring outbreaks, has received little experimental evaluation as a primary agent of bighorn sheep pneumonia. In two experiments, bighorn sheep housed in multiple pens 7.6 to 12 m apart were exposed to M. ovipneumoniae by introduction of a single infected or challenged animal to a single pen. Respiratory disease was monitored by observation of clinical signs and confirmed by necropsy. Bacterial involvement in the pneumonic lungs was evaluated by conventional aerobic bacteriology and by culture-independent methods. In both experiments the challenge strain of M. ovipneumoniae was transmitted to all animals both within and between pens and all infected bighorn sheep developed bronchopneumonia. In six bighorn sheep in which the disease was allowed to run its course, three died with bronchopneumonia 34, 65, and 109 days after M. ovipneumoniae introduction. Diverse bacterial populations, predominantly including multiple obligate anaerobic species, were present in pneumonic lung tissues at necropsy. Exposure to a single M. ovipneumoniae infected animal resulted in transmission of infection to all bighorn sheep both within the pen and in adjacent pens, and all infected sheep developed bronchopneumonia. The epidemiologic, pathologic and microbiologic findings in these experimental animals resembled those seen in naturally occurring pneumonia outbreaks in free ranging bighorn sheep.

  7. Mycoplasma pneumoniae CARDS toxin is internalized via clathrin-mediated endocytosis.

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    Manickam Krishnan

    Full Text Available Bacterial toxins possess specific mechanisms of binding and uptake by mammalian cells. Mycoplasma pneumoniae CARDS (Community Acquired Respiratory Distress Syndrome toxin is a 68 kDa protein, which demonstrates high binding affinity to human surfactant protein-A and exhibits specific biological activities including mono-ADP ribosylation and vacuolization. These properties lead to inflammatory processes in the airway and a range of cytopathologies including ciliostasis, loss of tissue integrity and injury, and cell death. However, the process by which CARDS toxin enters target cells is unknown. In this study, we show that CARDS toxin binds to mammalian cell surfaces and is internalized rapidly in a dose and time-dependent manner using a clathrin-mediated pathway, as indicated by inhibition of toxin internalization by monodansylcadaverine but not by methyl-β-cyclodextrin or filipin. Furthermore, the internalization of CARDS toxin was markedly inhibited in clathrin-depleted cells.

  8. Identification, expression and serological evaluation of the recombinant ATP synthase beta subunit of Mycoplasma pneumoniae

    Directory of Open Access Journals (Sweden)

    Nuyttens Hélène

    2010-08-01

    Full Text Available Abstract Background Mycoplasma pneumoniae is responsible for acute respiratory tract infections (RTIs common in children and young adults. As M. pneumoniae is innately resistant to β-lactams antibiotics usually given as the first-line treatment for RTIs, specific and early diagnosis is important in order to select the right treatment. Serology is the most used diagnostic method for M. pneumoniae infections. Results In this study, we identified the M. pneumoniae ATP synthase beta subunit (AtpD by serologic proteome analysis and evaluated its usefulness in the development of a serological assay. We successfully expressed and purified recombinant AtpD (rAtpD protein, which was recognised by serum samples from M. pneumoniae-infected patient in immunoblots. The performance of the recombinant protein rAtpD was studied using a panel of serum samples from 103 infected patients and 86 healthy blood donors in an in-house IgM, IgA and IgG enzyme-linked immunosorbent assay (ELISA. The results of this assay were then compared with those of an in-house ELISA with a recombinant C-terminal fragment of the P1 adhesin (rP1-C and of the commercial Ani Labsystems ELISA kit using an adhesin P1-enriched whole-cell extract. Performances of the rAtpD and rP1-C antigen combination were further assessed by binary logistic regression analysis. We showed that combination of rAtpD and rP1-C discriminated maximally between the patients infected with M. pneumoniae (children and adults and the healthy subjects for the IgM class, performing better than the single recombinant antigens or the commercial whole-cell extract. Conclusion These results suggest that AtpD can be used as an antigen for the immunodiagnosis of early and acute M. pneumoniae infection in association with adhesin P1, providing an excellent starting point for the development of point-of-care diagnostic assays.

  9. Molecular Epidemiology of Mycoplasma pneumoniae: Genotyping Using Single Nucleotide Polymorphisms and SNaPshot Technology

    Science.gov (United States)

    Touati, A.; Blouin, Y.; Sirand-Pugnet, P.; Renaudin, H.; Oishi, T.; Vergnaud, G.; Bébéar, C.

    2015-01-01

    Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M. pneumoniae strains, using the SNaPshot minisequencing assay. Eight SNPs, localized in housekeeping genes, predicted lipoproteins, and adhesin P1 genes were selected for genotyping. These SNPs were evaluated on 140 M. pneumoniae clinical isolates previously genotyped by multilocus variable-number tandem-repeat analysis (MLVA-5) and adhesin P1 typing. This method was also adapted for direct use with clinical samples and evaluated on 51 clinical specimens. The analysis of the clinical isolates using the SNP typing method showed nine distinct SNP types with a Hunter and Gaston diversity index (HGDI) of 0.836, which is higher than the HGDI of 0.583 retrieved for the MLVA-4 typing method, where the nonstable Mpn1 marker was removed. A strong correlation with the P1 adhesin gene typing results was observed. The congruence was poor between MLVA-5 and SNP typing, indicating distinct genotyping schemes. Combining the results increased the discriminatory power. This new typing method based on SNPs and the SNaPshot technology is a method for rapid M. pneumoniae typing directly from clinical specimens, which does not require any sequencing step. This method is based on stable markers and provides information distinct from but complementary to MLVA typing. The combined use of SNPs and MLVA typing provides powerful discrimination of strains. PMID:26202117

  10. Molecular Epidemiology of Mycoplasma pneumoniae: Genotyping Using Single Nucleotide Polymorphisms and SNaPshot Technology.

    Science.gov (United States)

    Touati, A; Blouin, Y; Sirand-Pugnet, P; Renaudin, H; Oishi, T; Vergnaud, G; Bébéar, C; Pereyre, S

    2015-10-01

    Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M. pneumoniae strains, using the SNaPshot minisequencing assay. Eight SNPs, localized in housekeeping genes, predicted lipoproteins, and adhesin P1 genes were selected for genotyping. These SNPs were evaluated on 140 M. pneumoniae clinical isolates previously genotyped by multilocus variable-number tandem-repeat analysis (MLVA-5) and adhesin P1 typing. This method was also adapted for direct use with clinical samples and evaluated on 51 clinical specimens. The analysis of the clinical isolates using the SNP typing method showed nine distinct SNP types with a Hunter and Gaston diversity index (HGDI) of 0.836, which is higher than the HGDI of 0.583 retrieved for the MLVA-4 typing method, where the nonstable Mpn1 marker was removed. A strong correlation with the P1 adhesin gene typing results was observed. The congruence was poor between MLVA-5 and SNP typing, indicating distinct genotyping schemes. Combining the results increased the discriminatory power. This new typing method based on SNPs and the SNaPshot technology is a method for rapid M. pneumoniae typing directly from clinical specimens, which does not require any sequencing step. This method is based on stable markers and provides information distinct from but complementary to MLVA typing. The combined use of SNPs and MLVA typing provides powerful discrimination of strains. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  11. Pneumonia of lambs in the Abruzzo region of Italy: anatomopathological and histopathological studies and localisation of Mycoplasma ovipneumoniae.

    Science.gov (United States)

    Ettorre, Chiara; Sacchini, Flavio; Scacchia, Massimo; Della Salda, Leonardo

    2007-01-01

    The most common forms of inflammation of the lower respiratory tract in lambs are acute enzootic pneumonia, caused mainly by Mannheimia haemolytica, chronic enzootic pneumonia (defined as 'atypical' in lambs), the aetiological of which is Mycoplasma ovipneumoniae and viral inflammation principally caused by parainfluenza virus type 3. The authors conducted anatomopathological and histopathological studies of the most commonly encountered spontaneous lung inflammations in lambs slaughtered in the Abruzzo region of Italy, with special attention to 'atypical pneumonia'. Microbiological isolations and a histopathological and immunohistochemical analysis were performed to reveal any possible correlations between causal agents and lesion patterns. Positive results for M. ovipneumoniae were compared to those for Mycoplasma isolation to evaluate the sensitivity of the two techniques. Of a total of 156 samples, 31 (19.8%) demonstrated involvement of M. ovipneumoniae, 15 (9.6%) were positive on microbiological isolation confirmed by typing with biomolecular methods and, finally, histological lesions (atypical pneumonia) were observed in the remaining 16 cases (10.2%). Of these 31 samples, 23 (14.7% of the total) demonstrated postive antigen in alveolar macrophages and giant cells on immunohistochemical testing. These data revealed the presence of chronic enzootic pneumonia in the Abruzzo area and the importance of immunohistochemistry (in combination with isolation and anatomopathological and histopathological examination) for the diagnosis of pneumonia caused by M. ovipneumoniae, as well as the high sensitivity shown by antigen marker expression, even in samples where bacterial load was limited.

  12. Quantificação morfométrica de Chlamydia pneumoniae e Mycoplasma pneumoniae em aneurismas de aorta abdominal humana Morphometrical quantification of Chlamydia pneumoniae and Mycoplasma pneumoniae in human atherosclerotic abdominal aortic aneurysms

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    Lucas José Tachotti Pires

    2007-09-01

    Full Text Available OBJETIVO: A inflamação aterosclerótica, com possível papel de agentes infecciosos, pode contribuir na patogênese dos aneurismas da aorta abdominal (AAA. O achado de Chamydia pneumoniae (CP nessas lesões, em estudos prévios, sem quantificação, variou de 0-100%. O objetivo é quantificar a presença de CP e de Mycoplasma pneumoniae (MP nos AAA. MÉTODO: A espessura, o número de células positivas para CP detectadas por imunoperoxidase e a porcentagem de área ocupada por MP detectada por hibridização "in situ", nas três camadas da aorta, foram medidos com sistema de análise de imagens, em 10 aortas abdominais aneurismáticas. Usouse três grupos-controle: 1 amostras das mesmas aortas, fora do aneurisma, exceto se a dilatação tomasse toda a porção sub-renal da artéria (n=7; 2 aortas com aterosclerose grave, mas sem aneurismas (n=10; 3 aortas sem aterosclerose ou com grau leve da doença (n=10. Todos os espécimes foram obtidos em necropsias. Usou-se o teste de Wald para comparar os grupos; fixou-se o nível de significância em 5%. RESULTADOS: A íntima era mais fina e a média mais espessa nos casos normais que nos outros grupos (p0,05. Também se detectou MP em todos os grupos. Este agente predominou no grupo de pacientes com aterosclerose, mas sem aneurisma na íntima e na adventícia; entretanto, as diferenças entre os grupos não foram significativas (p>0,05. CONCLUSÕES: Nossos dados sugerem que os agentes enfocados não têm papel importante na patogênese dos AAA.OBJECTIVE: Atherosclerotic inflammation, with a possible role of infectious agents, could contribute to the pathogenesis of abdominal aortic aneurysms (AAA. Finding of Chlamydia pneumoniae (CP in these lesions in previous, non-quantifying studies ranged from 0-100%. The objective is to quantify the presence of CP and Mycoplasma pneumoniae (MP in AAA. METHODS: Thickness, number of cells positive for CP by immunohistochemistry and percent area occupied by MP

  13. Preparation and analysis of immunocompetence of recombinant fusion protein of the immunodominant region in chlamydial protease-like activity factor from Chlamydophila pneumoniae and its application in serodiagnosis

    Institute of Scientific and Technical Information of China (English)

    JIANG HUA ZHENG; YI MOU WU; TAO DING; LI LI CHEN; JIA QIANG LIU; SHUANG QUAN LIU

    2007-01-01

    To clone the gene coding the immunodominant region in the chlamydial protease-like activity factor (CPAF) from Chlamydophila pneumoniae, to analyze immunocompetence of the expressed protein,and to evaluate its value in serodiagnosis, the CPAF immunodominant region gene was amplified, ligated into a pGEX6p-2 vector, and then the expressed recombinant protein was purified with glutathione Stransferase (GST) agarose gel FF after renaturation, then identified by SDS-PAGE and Western blot. A new indirect ELISA was developed with the purified protein as coating antigen. The immunogenicity of the recombinant protein was evaluated by immunization to New Zealand rabbits, and its immunoreactivity was analyzed by reacting with anti-C, pneumoniae antibody. 300 clinical sera samples were respectively detected by microimmunofluorescence (MIF) as reference method and the indirect ELISA, and the difference between the two methods was analyzed. Cross-reactivity against Chlamydia trachomatis was investigated with the indirect ELISA to detect anti-C, trachomatis positive antisera. The results indicated that a 51.3 kDa recombinant protein was obtained. Western blot assay proved that the recombinant protein could merely specifically react with human anti- C. pneumoniae antisera. The titers of the specific IgG antibodies in the immunized New Zealand rabbits were above 1 : 16 000. Anti- C. pneumoniae IgG positive and negative reference sera were detected with the indirect ELISA, and the concordance rate of negative and positive results were both 100% (40/40). The sensitivity and specificity of the indirect ELISA in comparison with MIF were 93.8% (45/48) and 100% (252/252) separately by detecting 300 clinical sera samples, and the concordance rate between the two methods was 99.0%. No cross reaction against C. trachomatis was found with the indirect ELISA to detect anti-C, trachomatis positive antisera. In conclusion, the prepared recombinant protein of the CPAF immunodominant region

  14. Structure of CARDS toxin, a unique ADP-ribosylating and vacuolating cytotoxin from Mycoplasma pneumoniae.

    Science.gov (United States)

    Becker, Argentina; Kannan, T R; Taylor, Alexander B; Pakhomova, Olga N; Zhang, Yanfeng; Somarajan, Sudha R; Galaleldeen, Ahmad; Holloway, Stephen P; Baseman, Joel B; Hart, P John

    2015-04-21

    Mycoplasma pneumoniae (Mp) infections cause tracheobronchitis and "walking" pneumonia, and are linked to asthma and other reactive airway diseases. As part of the infectious process, the bacterium expresses a 591-aa virulence factor with both mono-ADP ribosyltransferase (mART) and vacuolating activities known as Community-Acquired Respiratory Distress Syndrome Toxin (CARDS TX). CARDS TX binds to human surfactant protein A and annexin A2 on airway epithelial cells and is internalized, leading to a range of pathogenetic events. Here we present the structure of CARDS TX, a triangular molecule in which N-terminal mART and C-terminal tandem β-trefoil domains associate to form an overall architecture distinct from other well-recognized ADP-ribosylating bacterial toxins. We demonstrate that CARDS TX binds phosphatidylcholine and sphingomyelin specifically over other membrane lipids, and that cell surface binding and internalization activities are housed within the C-terminal β-trefoil domain. The results enhance our understanding of Mp pathogenicity and suggest a novel avenue for the development of therapies to treat Mp-associated asthma and other acute and chronic airway diseases.

  15. Progress on the treatment of mycoplasma pneumoniae pneumonia%肺炎支原体肺炎治疗新进展

    Institute of Scientific and Technical Information of China (English)

    张锡莲

    2011-01-01

    肺炎支原体是儿童社区获得性肺炎的常见病原.近年来肺炎支原体肺炎(mycoplasmapneumoniae pneumonia,MPP)的发病率逐年增高,国内外关于难治性MPP的病例报道也明显增多,MPP的治疗成为临床医师关注的热点.本文就MPP的抗生素治疗、免疫治疗及其他辅助治疗进展作一综述.%Mycoplasma pneumoniae is the common pathogen of community-acquired pneumonia in children. In recent years,the incidence of mycoplasma pneumoniae pneumonia(MPP) has increased year by year. And it is found that case reports about intractable MPP at home and abroad have also significantly increased. The treatments of MPP become the focus in clinical physicians. This paper summarized about progress of MPP in antibiotic therapy,immune therapy and other ancillary treatment.

  16. Effect of montelukast combined with procaterol treatment on airway remodeling and inflammatory response in children with mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    Jian-Mei Zhang; Fen-Xia Zhang; Yan Jia; Min Wang; Jue Bai

    2016-01-01

    Objective:To study the effect of montelukast combined with procaterol treatment on airway remodeling and inflammatory response in children with mycoplasma pneumonia.Methods:A total of 60 mycoplasma pneumonia children with acute asthma attack treated in our hospital were selected and randomly divided into two groups, observation group received conventional anti-infection, cough-relieving and sputum-reducing combined with montelukast and procaterol spasmolysis and asthma-relieving treatment, control group received conventional anti-infection, cough-relieving and sputum-reducing combined with procaterol spasmolysis and asthma-relieving treatment, and then the degree of airway remodeling and inflammatory response was compared between two groups.Results:Serum MCP-4, MDC and CysLTs levels of both groups 1 week after treatment were significantly lower than those before treatment and serum MCP-4, MDC and CysLTs levels of observation group after treatment were lower than those of control group; 1 week after treatment, LD and the proportion of Treg in induced sputum of observation group were significantly higher than those of control group, WT, WA%, PEF25, PEF50 and PEF75 as well as FN1 and Col-1 levels and the proportion of Th17 in induced sputum were significantly lower than those of control group, and FVC, PEF and FEV1 were not different from those of control group.Conclusions:Montelukast combined with procaterol treatment can significantly improve the airway remodeling and inflammatory response in mycoplasma pneumonia children with acute asthma attack.

  17. Tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses.

    Science.gov (United States)

    Hannan, P C; Bhogal, B S; Fish, J P

    1982-07-01

    The effects of tylosin tartrate and tiamutilin were examined in pneumonias induced experimentally in neonatal piglets with a homogenate of pneumonic pig lung, obtained from pigs with naturally acquired enzootic pneumonia. The homogenate contained mycoplasmas, including Mycoplasma hyopneumoniae (M suipneumoniae) and M hyorhinis, and certain bacteria and viruses. The experimental pneumonias generally resembled mycoplasmal pneumonia histologically but were complicated by aspiration pneumonia in some animals. both tylosin tartrate (50 mg/kg) and tiamutilin (10 mg/kg) administered orally twice daily for 10 days, beginning 14 days after intranasal infection, significantly reduced the incidence and severity of macroscopical pneumonic lung lesions. M hyopneumoniae could be isolated from the lungs of the unmedicated piglets, but not from drug-treated piglets. The numbers of M hyorhinis, Acholeplasma granularum, Haemophilus parasuis, Pasteurella multocida and P haemolytica in the lung tissue of the infected piglets were significantly reduced by drug therapy. The role of bacterial in the experimental infection appeared to be that of secondary invaders.

  18. 肺炎支原体肺炎及其并发症的诊治进展%Advance in the diagnosis and treatment of Mycoplasma pneumoniae pneumonia and related complications

    Institute of Scientific and Technical Information of China (English)

    张永明

    2011-01-01

    @@ 肺炎支原体肺炎(Mycoplasma pneumoniae pneumonia, MPP)为5岁以上儿童及青少年中最常见的下呼吸道感染性疾病.文献报道,肺炎支原体(My-coplasma pneumoniae, MP)感染占儿童社区获得性肺炎(community acquired pneumonia, CAP)病原的10%-40%以上[1].

  19. Epizootic pneumonia of bighorn sheep following experimental exposure to Mycoplasma ovipneumoniae.

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    Thomas E Besser

    Full Text Available Bronchopneumonia is a population limiting disease of bighorn sheep (Ovis canadensis. The cause of this disease has been a subject of debate. Leukotoxin expressing Mannheimia haemolytica and Bibersteinia trehalosi produce acute pneumonia after experimental challenge but are infrequently isolated from animals in natural outbreaks. Mycoplasma ovipneumoniae, epidemiologically implicated in naturally occurring outbreaks, has received little experimental evaluation as a primary agent of bighorn sheep pneumonia.In two experiments, bighorn sheep housed in multiple pens 7.6 to 12 m apart were exposed to M. ovipneumoniae by introduction of a single infected or challenged animal to a single pen. Respiratory disease was monitored by observation of clinical signs and confirmed by necropsy. Bacterial involvement in the pneumonic lungs was evaluated by conventional aerobic bacteriology and by culture-independent methods. In both experiments the challenge strain of M. ovipneumoniae was transmitted to all animals both within and between pens and all infected bighorn sheep developed bronchopneumonia. In six bighorn sheep in which the disease was allowed to run its course, three died with bronchopneumonia 34, 65, and 109 days after M. ovipneumoniae introduction. Diverse bacterial populations, predominantly including multiple obligate anaerobic species, were present in pneumonic lung tissues at necropsy.Exposure to a single M. ovipneumoniae infected animal resulted in transmission of infection to all bighorn sheep both within the pen and in adjacent pens, and all infected sheep developed bronchopneumonia. The epidemiologic, pathologic and microbiologic findings in these experimental animals resembled those seen in naturally occurring pneumonia outbreaks in free ranging bighorn sheep.

  20. Structural Study of MPN387, an Essential Protein for Gliding Motility of a Human-Pathogenic Bacterium, Mycoplasma pneumoniae.

    Science.gov (United States)

    Kawakita, Yoshito; Kinoshita, Miki; Furukawa, Yukio; Tulum, Isil; Tahara, Yuhei O; Katayama, Eisaku; Namba, Keiichi; Miyata, Makoto

    2016-09-01

    Mycoplasma pneumoniae is a human pathogen that glides on host cell surfaces with repeated catch and release of sialylated oligosaccharides. At a pole, this organism forms a protrusion called the attachment organelle, which is composed of surface structures, including P1 adhesin and the internal core structure. The core structure can be divided into three parts, the terminal button, paired plates, and bowl complex, aligned in that order from the front end of the protrusion. To elucidate the gliding mechanism, we focused on MPN387, a component protein of the bowl complex which is essential for gliding but dispensable for cytadherence. The predicted amino acid sequence showed that the protein features a coiled-coil region spanning residue 72 to residue 290 of the total of 358 amino acids in the protein. Recombinant MPN387 proteins were isolated with and without an enhanced yellow fluorescent protein (EYFP) fusion tag and analyzed by gel filtration chromatography, circular dichroism spectroscopy, analytical ultracentrifugation, partial proteolysis, and rotary-shadowing electron microscopy. The results showed that MPN387 is a dumbbell-shaped homodimer that is about 42.7 nm in length and 9.1 nm in diameter and includes a 24.5-nm-long central parallel coiled-coil part. The molecular image was superimposed onto the electron micrograph based on the localizing position mapped by fluorescent protein tagging. A proposed role of this protein in the gliding mechanism is discussed. Human mycoplasma pneumonia is caused by a pathogenic bacterium, Mycoplasma pneumoniae This tiny, 2-μm-long bacterium is suggested to infect humans by gliding on the surface of the trachea through binding to sialylated oligosaccharides. The mechanism underlying mycoplasma "gliding motility" is not related to any other well-studied motility systems, such as bacterial flagella and eukaryotic motor proteins. Here, we isolated and analyzed the structure of a key protein which is directly involved in the

  1. Association of the ACE, GSTM1, IL-6, NOS3, and CYP1A1 polymorphisms with susceptibility of mycoplasma pneumoniae pneumonia in Chinese children.

    Science.gov (United States)

    Zhao, Jie; Zhang, Wen; Shen, Li; Yang, Xiaomeng; Liu, Yi; Gai, Zhongtao

    2017-04-01

    Mycoplasma pneumoniae is a common cause of community-acquired pneumonia (CAP) and the clinical presentation of mycoplasma pneumoniae pneumonia (MPP) varies widely. Genetic variability affecting the host response may also influence the susceptibility to MPP. Several studies have investigated the association between single nucleotide polymorphism (SNP) of some genes and the risks of CAP; however, the results were inconsistent. Here, we investigated the association of 5 functional genes and the risks of MPP, including ACE (rs4340), GSTM1 (Ins/del), IL-6 (rs1800795), NOS3 (rs1799983), and CYP1A1 (rs2606345) in a total of 715 subjects (415 cases, 300 controls) by using tetra-primer allele-specific polymerase chain reaction (PCR) and Sanger sequencing. The gene-gene interactions were analyzed using the Multifactor Dimensionality Reduction and cumulative genetic risk score approaches. Our results showed that 3 SNPs of ACE rs4340, IL-6 rs1800795, and NOS3 rs1799983 were significantly associated with the risks of MPP, while no differences were observed in genotype frequencies of GSTM1 (Ins/del) and CYP1A1 rs2606345 between both groups. The combinations of ACE rs4340D/NOS3 rs1799983T/CYP1A1 rs2606345G and ACE rs4340D/NOS3 rs1799983T contribute to the genetic susceptibility of MPP in Chinese children.

  2. Clinical Treatment and Therapy of Mycoplasma Pneumoniae Pneumonia%小儿肺炎支原体肺炎的临床诊断与治疗

    Institute of Scientific and Technical Information of China (English)

    黄洁明; 周健铖; 唐骏

    2014-01-01

    Objective:To study the methods and effects of clinical diagnosis and treatment of Mycoplasma pneumoniae pneumonia.Method:The clinical data of 64 cases of mycoplasma pneumoniae pneumonia from March 2011 to March 2014 in our hospital was retrospective analysed.Result:64 cases of mycoplasma pneumoniae pneumonia were laboratory examination of peripheral,white blood cells increased in 28.13%or decreased in 21.88%,ESR increased in 39.06%and spectrum of myocardial enzymes increased in 10.94%,X chest radiograph in foggy shadows 54.69%,chest door inflammation around 37.50%,and the bronchitis was 7.81%,the azithromycin sequential therapy of mycoplasma pneumoniae pneumonia in children the total clinical efficacy rate was 96.88%(62/64). Conclusion:Mycoplasma pneumoniae pneumonia incidence is higher,and the clinical manifestation is not typical,need to be confirmed by the clinical symptoms,disease history,laboratory examination,in general on the basis of conventional therapy,the better effect of azithromycin sequential therapy desirable.%目的:分析小儿肺炎支原体肺炎的临床诊断及治疗方法和效果。方法:2011年3月-2014年3月笔者所在医院收治的肺炎支原体肺炎患儿64例,对其临床资料进行回顾性分析。结果:64例肺炎支原体肺炎患儿实验室检查中外周血白细胞升高28.13%,外周血白细胞降低21.88%,血沉增快39.06%,心肌酶谱增高10.94%;X胸片检查中云雾状阴影54.69%,肺门周围炎37.50%,支气管炎7.81%;采用阿奇霉素序贯疗法治疗小儿肺炎支原体肺炎临床总有效率96.88%(62/64)。结论:小儿肺炎支原体肺炎的发病率较高,且临床表现不典型,需要通过临床症状、疾病史、实验室检查等进行确诊,在常规治疗基础上,采用阿奇霉素序贯疗法治疗可取得较好效果。

  3. Cellular vacuoles induced by Mycoplasma pneumoniae CARDS toxin originate from Rab9-associated compartments.

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    Coreen Johnson

    Full Text Available Recently, we identified an ADP-ribosylating and vacuolating cytotoxin in Mycoplasma pneumoniae designated Community Acquired Respiratory Distress Syndrome (CARDS toxin. In this study we show that vacuoles induced by recombinant CARDS (rCARDS toxin are acidic and derive from the endocytic pathway as determined by the uptake of neutral red and the fluid-phase marker, Lucifer yellow, respectively. Also, we demonstrate that the formation of rCARDS toxin-associated cytoplasmic vacuoles is inhibited by the vacuolar ATPase inhibitor, bafilomycin A1, and the ionophore, monensin. To examine the ontogeny of these vacuoles, we analyzed the distribution of endosomal and lysosomal membrane markers during vacuole formation and observed the enrichment of the late endosomal GTPase, Rab9, around rCARDS toxin-induced vacuoles. Immunogold-labeled Rab9 and overexpression of green fluorescent-tagged Rab9 further confirmed vacuolar association. The late endosomal- and lysosomal-associated membrane proteins, LAMP1 and LAMP2, also localized to the vacuolar membranes, while the late endosomal protein, Rab7, and early endosomal markers, Rab5 and EEA1, were excluded. HeLa cells expressing dominant-negative (DN Rab9 exhibited markedly reduced vacuole formation in the presence of rCARDS toxin, in contrast to cells expressing DN-Rab7, highlighting the importance of Rab9 function in rCARDS toxin-induced vacuolation. Our findings reveal the unique Rab9-association with rCARDS toxin-induced vacuoles and its possible relationship to the characteristic histopathology that accompanies M. pneumoniae infection.

  4. Detection of genetic mutations associated with macrolide resistance of Mycoplasma pneumoniae

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    Chi Eun Oh

    2010-02-01

    Full Text Available Purpose : The aim of this study was to identify mutations associated with macrolide resistance in Mycoplasma pneumoniae (MP and to establish a cultural method to determine antimicrobial susceptibility. Methods : Nasopharyngeal aspirates (NPAs were collected from 62 children diagnosed with MP pneumonia by a serologic method or polymerase chain reaction. The 23S rRNA and L4 ribosomal protein genes of MP were amplified and sequenced. To identify mutations in these 2 genes, their nucleotide sequences were compared to those of the reference strain M129. MP cultivation was carried out for 32 (28 frozen and 5 refrigerated NPAs and M129 strain using Chanock’s glucose broth and agar plate in a 5% CO2 incubator at 37?#608;and examined at 2-3 day intervals for 6 weeks. Results : Among the 62 specimens, 17 had M144V mutations in ribosomal protein L4. The A2064G mutation was observed in 1 specimen; its 23S rRNA gene was successfully sequenced. Culture for MP was successful from the M129 strain and 2 of the 5 NPAs that were refrigerated for no longer than 3 days. However, MP did not grow from the 28 NPAs that were kept frozen at -80?#608;since 2003. Conclusion : We found the M144V mutation of L4 protein to be common and that of domain V of 23S rRNA gene was relatively rare among MP. Studies on the prevalence of macrolide-resistant MP and the relationship between the mutations of 23S rRNA gene and ribosomal protein L4 will aid in understanding the mechanism of macrolide resistance in MP.

  5. Molecular Detection,Culture and Isolation of Mycoplasma Pneumoniae From Reproductive Tract of STD Patients

    Institute of Scientific and Technical Information of China (English)

    WANG Jidong(王继东); ZHAO Jiwen(赵季文); LI Qin(李琴); XU Cuiyu(徐萃瑜); XIE Ping(谢平); HUA Yong(华咏); WANG Shengqiang(汪圣强); XIAO Chenyue(肖琛月)

    2002-01-01

    Objective: To confirm whether Mycoplasma pneumoniae(MP) are present in reproductive tract of STD patients in China.Methods: Application of nested PCR (nPCR) and DNA sequencing to test samples of urethral/vaginal swabs withMP culture confirmation of several nPCR positive patients.Results: 74 of 786 STD patients were positive for MP bynPCR, with a rate of 9.4%. Of the 484 male patients, 10.5%were positive, and among the 302 female patients, 7.6%were positive. There was no significant difference betweenthem (P>0.05). Of 12 cases of MP positive samples by nPCR,4 cases were first generation culture-positive, and one ofthem passed to the next generation successfully. DNAsequencing was performed on the nPCR product of oneswab sample and one MP culture isolation. The determinedsequence was identical to the typical MP strain.Conclusion: In China, MP are present in reproductivetract of both male and female STD patients.

  6. Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine.

    Science.gov (United States)

    Borjigin, Liushiqi; Shimazu, Tomoyuki; Katayama, Yuki; Li, Meihua; Satoh, Takumi; Watanabe, Kouichi; Kitazawa, Haruki; Roh, Sang-gun; Aso, Hisashi; Katoh, Kazuo; Uchida, Takafumi; Suda, Yoshihito; Sakuma, Akiko; Nakajo, Mituru; Suzuki, Keiichi

    2016-03-01

    Mycoplasma pneumonia of swine (MPS) lung lesions and immunogenic properties were compared between a Landrace line that was genetically selected for reduced incidence of pulmonary MPS lesions, and a non-selected Landrace line. The MPS-selected Landrace line showed significantly lower degrees of pulmonary MPS lesions compared with the non-selected Landrace line. When changes in immunity before and after vaccination were compared, the percentage of B cells in the peripheral blood of the MPS-selected Landrace line was significantly lower than that of the non-selected line. Furthermore, the concentration of growth hormone and the mitogen activity of peripheral blood mononuclear cells in the MPS-selected Landrace line showed significantly (P Landrace line than in the non-selected line. Gene expression of toll-like receptor (TLR)2 and TLR4 was significantly higher in the MPS-selected Landrace line in immune tissues, with the exception of the hilar lymph nodes. The present results suggest that peripheral blood IFN-γ, salivary IgA TLR2, and TLR4 are important immunological factors influencing the development of MPS lesions.

  7. Bronchiolitis associated with Mycoplasma pneumoniae in infants in Suzhou China between 2010 and 2012.

    Science.gov (United States)

    Wang, Yuqing; Hao, Chuangli; Ji, Wei; Yan, Yongdong; Shao, Xuejun; Xu, Jun

    2015-01-19

    Viruses cause most cases of bronchiolitis in infants; consequently the importance of other agents such as Mycoplasma pneumoniae (MP) in the etiology of bronchiolitis may not be fully recognized. We investigated the prevalence and seasonal distribution of bronchiolitis caused by MP in 674 children admitted to the Children's Hospital affiliated with Soochow University from January 2010 to December 2012. The presence of MP was confirmed by real-time PCR. During the 3 years, we identified MP in 17.2% of the children with bronchiolitis. The annual MP detection rates were 16.6% in 2010, 17.8% in 2011, and 17.2% in 2012. MP was detected throughout the year, with a peak from July to September. The median age of MP-positive children was 10 months. Common clinical manifestations included cough, wheezing, and high fever. Moist and/or wheezing rales were frequent, and pulmonary interstitial infiltration was seen in 66.4% of chest X-rays. Patients with MP infection were older, were more likely to have pulmonary interstitial infiltration, and had shorter hospital stays than those with respiratory syncytial virus infection. Our study revealed MP as an important cause of bronchiolitis, with peaks of occurrence during the summer and early autumn. Pulmonary interstitial infiltrations were a common event.

  8. Isolation and immunological detection of Mycoplasma ovipneumoniae in sheep with atypical pneumonia, and lack of a role for Mycoplasma arginini.

    Science.gov (United States)

    Lin, Y-C; Miles, R J; Nicholas, R A J; Kelly, D P; Wood, A P

    2008-06-01

    Mycoplasma ovipneumoniae NCTC 10151(T) and four new isolates from UK sheep flocks were compared. Only glucose and pyruvate were used as energy sources by the five strains: glucose was the best energy source for the type strain, pyruvate supported better growth of the new strains. Whole cell protein patterns and antigenic profiles showed high similarity between all five strains. The new isolates fell into two groups in ELISA tests. Serum samples from 30 pneumonic sheep were assessed for M. ovipneumoniae infection and Mycoplasma arginini co-infection. Fourteen (out of 30) serum samples were positive for M. ovipneumoniae both by ELISA and immunoblotting. Twelve antigenic proteins of M. ovipneumoniae were detected in infected serum samples: the antigen patterns were unique, with between one and at least seven occurring in any one sample. All serum samples were designated as negative for M. arginini antibodies by both ELISA and immunoblotting.

  9. Rare zoonosis (hemotrophic mycoplasma infection in a newly diagnosed systemic lupus erythematosus patient followed by a Nocardia asteroides pneumonia

    Directory of Open Access Journals (Sweden)

    Dubravka Bosnic

    2010-02-01

    Full Text Available Systemic lupus erythematosus (SLE is per se a disease characterized by suppressed immune response and thus susceptibility to various opportunistic infections. We describe the case of a 21-yearold woman who developed a rare zoonosis - hemotrophic mycoplasma infection in the initial stage of SLE, complicated with Nocardia asteroides pneumonia afterwards. Nocardia infection coincided with initiation of glucocorticoids and cyclophosphamide therapy for SLE. After the treatment she recovered completely. To our knowledge the only case of human hemoplasmosis (then referred to as eperythrozoonosis in medical literature was the one described by a group of Croatian authors 22 years ago. No cases of a hemotrophic mycoplasma infection in a SLE patient have been published up to now.

  10. Therapeutic efficacy of azithromycin, clarithromycin, minocycline and tosufloxacin against macrolide-resistant and macrolide-sensitive Mycoplasma pneumoniae pneumonia in pediatric patients

    Science.gov (United States)

    Koseki, Naoko; Kaiho, Miki; Ariga, Tadashi; Kikuta, Hideaki; Togashi, Takehiro; Oba, Koji; Morita, Keisuke; Nagano, Naoko; Nakanishi, Masanori; Hara, Kazuya; Hazama, Kyosuke; Watanabe, Toru; Yamanaka, Tatsuru; Sasaki, Satoshi; Furuyama, Hideto; Shibata, Mutsuo; Shida, Satoru; Ishizaka, Akihito; Tabata, Yuichi; Aoyagi, Hayato; Naito, Hiroyuki; Yoshioka, Mikio; Horino, Atsuko; Kenri, Tsuyoshi

    2017-01-01

    Objective To clarify therapeutic effects of azithromycin, clarithromycin, minocycline and tosufloxacin against macrolide-resistant Mycoplasma pneumoniae (MRMP) pneumonia and against macrolide-sensitive Mycoplasma pneumoniae (MSMP) pneumonia in pediatric patients. Methods A prospective, multicenter observational study was conducted from July 2013 to August 2015. The therapeutic effects of azithromycin, clarithromycin, minocycline and tosufloxacin were evaluated in 59 patients with pneumonia caused by MRMP and in 50 patients with pneumonia caused by MSMP. In vitro activities of antimicrobial agents against isolates of Mycoplasma pneumoniae were also measured. Results Mean durations of fever following commencement of treatment in patients infected with MRMP and MSMP were 5.2 and 1.9 days, respectively (log-rank test, P < 0.0001). Among patients infected with MRMP, mean durations of fever were 4.6, 5.5, 1.0 and 7.5 days for patients treated with azithromycin, clarithromycin, minocycline and tosufloxacin, respectively (log-rank test, P < 0.0001). Among patients infected with MSMP, mean durations of fever were 2.5, 1.7, 0.9 and 4.3 days for patients treated with azithromycin, clarithromycin, minocycline and tosufloxacin, respectively (log-rank test, P = 0.0162). The MIC90s of azithromycin and clarithromycin among the 27 isolates of MRMP were 64 and 256 μg/ml, respectively, and those among the 23 isolates of MSMP were <0.000125 and 0.001 μg/ml, respectively. The MIC90s of minocycline and tosufloxacin among the 27 isolates of MRMP were 1.0 and 0.25 μg/ml, respectively, and those among the 23 isolates of MSMP were 1.0 and 0.5 μg/ml, respectively. Conclusion Both minocycline and tosufloxacin showed good in vitro activities against MRMP. Minocycline, but not tosufloxacin, shortened the duration of fever in pediatric patients infected with MRMP compared to the duration of fever in patients treated with macrolides. PMID:28288170

  11. Prevalence of feline herpesvirus-1, feline calicivirus, Chlamydophila felis and Mycoplasma felis DNA and associated risk factors in cats in Spain with upper respiratory tract disease, conjunctivitis and/or gingivostomatitis.

    Science.gov (United States)

    Fernandez, Mireia; Manzanilla, Edgar G; Lloret, Albert; León, Marta; Thibault, Jean-Christophe

    2017-04-01

    Objectives Our objective was to perform the first multicentric study in Spain to evaluate the prevalence of feline herpesvirus-1 (FHV-1), feline calicivirus (FCV), Chlamydophila felis and Mycoplasma felis in cats with upper respiratory tract disease (URTD), conjunctivitis and/or gingivostomatitis (GS) compared with control cats; and to evaluate risk factors for these clinical conditions. Methods Conjunctival and oropharyngeal swabs were collected and a questionnaire regarding signalment, lifestyle, vaccination history and clinical signs was obtained for each cat. Swabs were tested for each pathogen by real-time PCR. Results The study population consisted of 358 cats, including 98 control cats. Among the 260 diseased cats, 127 cats presented with URTD, 149 cats had conjunctivitis, 154 cats were suffering GS; many cats presented more than one clinical condition. The prevalence observed of FHV-1, FCV, C felis and M felis was, respectively, 28.3%, 48.0%, 20.5% and 46.5% in cats with URTD; 24.2%, 43.6%, 19.5% and 38.3% in cats with conjunctivitis; and 15.6%, 58.4%, 9.1% and 37.7% in cats with GS. Prevalences in the control group were 6.1%, 15.3%, 2.0% and 20.4%, respectively. Coinfections were common among all groups of cats. Risk factors were identified for all groups. FHV-1, FCV and C felis were associated with URTD and conjunctivitis. FCV was strongly associated with GS. M felis was present in a high percentage of the population in all groups, but its role in these clinical conditions remains uncertain. Vaccination was protective for URTD and GS but not for conjunctivitis. Conclusions and relevance This epidemiological study describes, for the first time, prevalence for FHV-1, FCV, C felis and M felis in Spain. In general, the prevalences found are similar to those reported in other countries. Factors associated with disease expression were also identified, which are relevant for practitioners.

  12. Risk factors and clinical significance of severe mycoplasma pneumoniae pneumonia%重症肺炎支原体肺炎危险因素分析及临床意义

    Institute of Scientific and Technical Information of China (English)

    荆小袁

    2016-01-01

    Mycoplasma pneumoniae pneumonias are common in children.In recent years,the incidence of refractory mycoplasma pneumonia has been increasing,and some of the severe children have a poor response to macrolide antibiotics.However,the effect of glucoconicoid therapy may have unexpected results.The recognition of severe mycoplasma pneumonia risk factors may contribute to intervent with glucocorticoid early,reduce the occurrence of severe mycoplasma pneumonia,and improve the prognosis.Clinical studies have found that clinical manifestations,imaging findings,laboratory tests and other factors are related to the occurrence of severe mycoplasma pneumonia.In this paper,the risk factors that may be associated with accurrence of severe mycoplasma pneumonias are reviewed.%肺炎支原体肺炎在儿童中较为常见.近年来难治性肺炎支原体肺炎日趋增多,其中部分重症患几虽经大环内酯类抗生素治疗仍难见效.然而糖皮质激素治疗有意想不到的效果.对重症肺炎支原体肺炎危险因素的识别,可能有助于早期予以激素干预,改善预后.临床研究发现,临床表现、影像学表现及实验室检查等多种指标与重症肺炎支原体肺炎的发生相关.该文对目前国内外研究所涉及的、可能与重症肺炎支原体肺炎发生相关的因素进行综述.

  13. Effect of Tanreqing injection on mycoplasma pneumonia in patients of hs-CRP, PCT, T cell subsets

    Institute of Scientific and Technical Information of China (English)

    Mei Bai

    2016-01-01

    Objective: To explore the effect of Tanreqing injection for treatment and its clinical significance through the changes of mycoplasma pneumonia patients with hs-CRP, PCT levels and cellular immune index. Method:A total of 114 cases of mycoplasma pneumonia, which were randomly divided into routine antibiotic treatment group (n=53) (control group), Tanreqing injection treatment group (n=61) (treatment group), to observe the two groups' hs-CRP and PCT levels in serum and the cellular immune regulation after treatment in patients, and contrast the curative effect. Results:the two groups before treatment, hs-CRP and PCT levels in serum had no significant difference;the treatment group after treatment, hs-CRP and PCT levels in serum were significantly lower than before treatment, and lower than that of the control group's;the control group after treatment of serum hs-CRP was significantly lower than that before treatment, compared to the before treatment, the level of PCT decreased slightly, but compared with before treatment there was no significant difference;after treatment, CD3+, CD4+, CD4+/CD8+, CD56+value were significantly higher than the control group's;CD8+and CD4+CD25+were significantly lower than the control group's;effective treatment group was significantly higher than the control group. The average hospitalization time was less than the control group's. Conclusion:Tanreqing injection adjuvant therapy could improve the treatment effect in mycoplasma pneumonia patients, regulate immune function, which was worth popularizing in clinical use.

  14. 70 Cases of Infantile Pneumonia Mycoplasma Pneumonia Treatment Effect%70例小儿肺炎支原体肺炎治疗效果

    Institute of Scientific and Technical Information of China (English)

    胡彦菊

    2015-01-01

    目的:研究小儿肺炎支原体肺炎的治疗效果。方法随机选取我院收治的小儿肺炎支原体肺炎患儿140例;分为两组,对照组单独采用阿奇霉素治疗,研究组则在对照组基础上联合痰热清治疗,回顾性分析两组临床治疗效果。结果治疗后,研究组体温恢复正常时间、咳嗽消失时间、肺部啰音消失时间及总有效率等,与对照组相比差异显著,存在统计学方面的意义(P<0.05)。结论给予小儿肺炎支原体肺炎患儿阿奇霉素联合痰热清治疗的效果较为显著,值得在临床上推广应用。%Objective To study the therapeutic effect of pediatric mycoplasma pneumoniae pneumonia. Methods Randomly selected from our hospital 140 cases of children with infantile pneumonia mycoplasma pneumonia;Divided into two groups, control group treated with azithromycin alone, the team is in the control group on the basis of combined phlegm heat treatment,two groups were retrospectively analyzed the clinical therapeutic effect. Results After treatment,the team body temperature returned to normal time,cough disappeared,pulmonary rales disappear time and total effective rate, significant difference compared with control group, there is statistical significance (P<0.05). Conclusion For pediatric mycoplasma pneumoniae pneumonia in children with azithromycin combined phlegm heat treatment effect is significant, worthy of popularization and application in clinic.

  15. Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection.

    Directory of Open Access Journals (Sweden)

    Kelley C Henderson

    Full Text Available Mycoplasma pneumoniae is a cell wall-less bacterial pathogen of the human respiratory tract that accounts for > 20% of all community-acquired pneumonia (CAP. At present the most effective means for detection and strain-typing is quantitative polymerase chain reaction (qPCR, which can exhibit excellent sensitivity and specificity but requires separate tests for detection and genotyping, lacks standardization between available tests and between labs, and has limited practicality for widespread, point-of-care use. We have developed and previously described a silver nanorod array-surface enhanced Raman Spectroscopy (NA-SERS biosensing platform capable of detecting M. pneumoniae with statistically significant specificity and sensitivity in simulated and true clinical throat swab samples, and the ability to distinguish between reference strains of the two main genotypes of M. pneumoniae. Furthermore, we have established a qualitative lower endpoint of detection for NA-SERS of < 1 genome equivalent (cell/μl and a quantitative multivariate detection limit of 5.3 ± 1 cells/μl. Here we demonstrate using partial least squares- discriminatory analysis (PLS-DA of sample spectra that NA-SERS correctly identified M. pneumoniae clinical isolates from globally diverse origins and distinguished these from a panel of 12 other human commensal and pathogenic mycoplasma species with 100% cross-validated statistical accuracy. Furthermore, PLS-DA correctly classified by strain type all 30 clinical isolates with 96% cross-validated accuracy for type 1 strains, 98% cross-validated accuracy for type 2 strains, and 90% cross-validated accuracy for type 2V strains.

  16. Mielite Transversa Associada a Infecção por Mycoplasma Pneumoniae: Caso Clínico

    OpenAIRE

    Guimarães, Paulo; Teixeira, Fernanda; Oliveira, Maria José; Temudo, Teresa; Costa, Gomes da

    2014-01-01

    Mycoplasma pneumoniae é um agente infeccioso frequentemente implicado em infecções respiratórias em crianças e adolescentes.Complicações não respiratórias podem ocorrer, nomeadamente mucocutâneas, cardíacas, articulares, hematológicas e neurológicas, entre as quais a mielite transversa. Esta é uma entidade relativamente rara em idade pediátrica, embora potencialmente grave. Apesar da sua patogenia ser desconhecida, tem sido associada a infecção (nomeadamente vírica ou por micoplasma), lupus e...

  17. Mielite Transversa Associada a Infecção por Mycoplasma Pneumoniae: Caso Clínico

    OpenAIRE

    Guimarães, Paulo; Teixeira, Fernanda; Oliveira, Maria José; Temudo, Teresa; Costa, Gomes da

    2014-01-01

    Mycoplasma pneumoniae é um agente infeccioso frequentemente implicado em infecções respiratórias em crianças e adolescentes.Complicações não respiratórias podem ocorrer, nomeadamente mucocutâneas, cardíacas, articulares, hematológicas e neurológicas, entre as quais a mielite transversa. Esta é uma entidade relativamente rara em idade pediátrica, embora potencialmente grave. Apesar da sua patogenia ser desconhecida, tem sido associada a infecção (nomeadamente vírica ou por micoplasma), lupus e...

  18. problems and countermeasures for Mycoplasma pneumoniae infection%肺炎支原体感染防治若干问题及对策

    Institute of Scientific and Technical Information of China (English)

    张振国; 邱作成; 刘柏宏; 乐德行

    2012-01-01

    目的 探讨肺炎支原体感染临床治疗中有效对策.方法 结合近代肺炎支原体感染治疗研究现状部分问题,运用中医辨证和辩病论治结合现代药理学研究进展方法,防治肺炎支原体感染.结果 中西医结合防治此病有效.结论 中西医结合辨证和辩病结合论治防治此病为有效对策.%OBJECTIVE To explore the effective countenneasures of pneumoniae infection in the clinics. METHODS With the current status of mycoplasma pneumoniae infection treatment, TCM syndrome differentiation and disease differentiation theory combined with modern pharmacology were conducted to explore the prevention and treatment measures for mycoplasma pneumoniae infection. RESULTS Traditional Chinese and western medicine is effective in treatment of mycoplasma pneumoniae infection. CONCLUSION Combining traditional Chinese and western medicine syndrome differentiation is effective in treatment of mycoplasma pneumoniae infection.

  19. Comparison of antigens of pneumonia-associated mycoplasma species by gel diffusion.

    Science.gov (United States)

    Ball, H J; Todd, D

    1978-09-01

    Comparison of fluorocarbon-extracted antigens of six mycoplasma species by double immunodiffusion and counterimmunodiffusion techniques revealed a close reciprocal relationship among Mycoplasma dispar, M. ovipneumoniae, and M. hyopneumoniae. A lesser degree of cross-reaction was also demonstrated between these three species and M. hyorhinis and M. bovoculi. The interrelationships were more clearly demonstrated by double immunodiffusion than by counterimmunodiffusion.

  20. Airway epithelial NF-κB activation promotes Mycoplasma pneumoniae clearance in mice.

    Directory of Open Access Journals (Sweden)

    Di Jiang

    Full Text Available Respiratory infections including atypical bacteria Mycoplasma pneumoniae (Mp contribute to the pathobiology of asthma and chronic obstructive pulmonary disease (COPD. Mp infection mainly targets airway epithelium and activates various signaling pathways such as nuclear factor κB (NF-κB. We have shown that short palate, lung, and nasal epithelium clone 1 (SPLUNC1 serves as a novel host defense protein and is up-regulated upon Mp infection through NF-κB activation in cultured human and mouse primary airway epithelial cells. However, the in vivo role of airway epithelial NF-κB activation in host defense against Mp infection has not been investigated. In the current study, we investigated the effects of in vivo airway epithelial NF-κB activation on lung Mp clearance and its association with airway epithelial SPLUNC1 expression.Non-antimicrobial tetracycline analog 9-t-butyl doxycycline (9-TB was initially optimized in mouse primary tracheal epithelial cell culture, and then utilized to induce in vivo airway epithelial specific NF-κB activation in conditional NF-κB transgenic mice (CC10-(CAIKKβ with or without Mp infection. Lung Mp load and inflammation were evaluated, and airway epithelial SPLUNC1 protein was examined by immunohistochemistry. We found that 9-TB treatment in NF-κB transgene positive (Tg+, but not transgene negative (Tg- mice significantly reduced lung Mp load. Moreover, 9-TB increased airway epithelial SPLUNC1 protein expression in NF-κB Tg+ mice.By using the non-antimicrobial 9-TB, our study demonstrates that in vivo airway epithelial NF-κB activation promotes lung bacterial clearance, which is accompanied by increased epithelial SPLUNC1 expression.

  1. Chlamydophila (Chlamydia) pneumoniae infection of human astrocytes and microglia in culture displays an active, rather than a persistent, phenotype.

    Science.gov (United States)

    Dreses-Werringloer, Ute; Gérard, Hervé C; Whittum-Hudson, Judith A; Hudson, Alan P

    2006-10-01

    The intracellular pathogen Chlamydia pneumoniae can cause persistent infections during which its morphologic, molecular, and pathogenic characteristics differ importantly from those of active infection. This bacterium was identified within astrocytes and microglia in the brain of late-onset Alzheimer disease patients. We investigated whether infection of these two host cell types displays an active or persistent growth phenotype. The human astrocytoma and microglioma cell lines U-87 MG and CHME-5 (respectively) and the human epithelial cell line HEp-2 were infected by the standard method with C pneumoniae strain AR-39. Cultures were harvested at 24, 48, and 72 hours postinfection and subjected to analysis of inclusion morphology. DNA and RNA were prepared from portions of each infected culture sample and analyzed for relative chromosome accumulation and presence or absence of several specific bacterial mRNAs. Astrocytes and microglial cells infected in vitro with C pneumoniae displayed inclusions that were indistinguishable from those characteristic of active infection of the standard HEp-2 host cell line. Real time polymerase chain reaction (PCR) showed that the relative accumulation of chlamydial chromosome over time during infection of these two cell lines also was virtually identical to that in actively infected HEp-2 cells. Reverse transcriptase PCR (RT-PCR) analyses showed that mRNA from ftsK, pyk, and other chlamydial genes whose expression is abrogated during persistent infection were easily identifiable in infected CHME-5 and U-87 MG cells. In cultured human astrocytes and microglia, C pneumoniae displays an active, not a persistent, growth phenotype. This indicates normal passage through the developmental cycle with its probable concomitant destruction by lysis of some portion of host cells at the termination of that cycle.

  2. To Explore the Clinical Effect of Mycoplasma Pneumoniae Pneumonia in Children%小儿肺炎支原体肺炎临床效果观察

    Institute of Scientific and Technical Information of China (English)

    柴方平

    2015-01-01

    目的:浅析小儿肺炎支原体肺炎临床效果观察。方法将我院收治的肺炎支原体肺炎小儿96例按照随机抽选的方式分为实验组、对照组和研究组,研究组给予中药治疗,实验组给予阿奇霉素,对照组给予红霉素治疗。结果阿奇霉素治疗效果最好,中药治疗不良反应最少。结论治疗小儿肺炎支原体肺炎,采用大环内酯类抗生素,适合的配置中药进行治疗,效果更佳。%ObjectiveTo explore the clinical effect of mycoplasma pneumoniae pneumonia in children.Methods All of the patients were divided into experimental group, control group and the study group randomly. The research group were treated by traditional Chinese medicine, the experimental group received azithromycin, and the control group was given the erythromycin.ResultsThe best therapeutic effect was in the experimental group with azithromycin. And the minimal adverse reaction was in the research group with Chinese medicine treatment. Conclusion The best treatment for children with Mycoplasma pneumoniae pneumonia is macrolide antibiotics with traditional Chinese medicine.

  3. 重视肺炎支原体的耐药问题%Drug resistance of mycoplasma pneumoniae should be stressed

    Institute of Scientific and Technical Information of China (English)

    佘丹阳

    2011-01-01

    @@ 肺炎支原体(Mycoplasma Pneumoniae)是社区获得性肺炎(Community-Acquired Pneumonia,CAP)的常见致病原.一项全球性CAP病原学调查结果显示,肺炎支原体肺炎占CAP的12%,在所有非典型病原体所致的CAP中超过50%[1].

  4. Mycoplasma ovipneumoniae--a primary cause of severe pneumonia epizootics in the Norwegian Muskox (Ovibos moschatus) population.

    Science.gov (United States)

    Handeland, Kjell; Tengs, Torstein; Kokotovic, Branko; Vikøren, Turid; Ayling, Roger D; Bergsjø, Bjarne; Sigurðardóttir, Olöf G; Bretten, Tord

    2014-01-01

    The Norwegian muskox (Ovibos moschatus) population lives on the high mountain plateau of Dovre and originates from animals introduced from Greenland. In the late summers of 2006 and 2012, severe outbreaks of pneumonia with mortality rates of 25-30% occurred. During the 2012 epidemic high quality samples from culled sick animals were obtained for microbiological and pathological examinations. High throughput sequencing (pyrosequencing) of pneumonic lung tissue revealed high concentrations of Mycoplasma ovipneumoniae in all six animals examined by this method and Pasteurella multocida subsp. multocida in four animals, whereas no virus sequences could be identified. Mycoplasma ovipneumoniae and P. multocida multocida were also isolated by culture. Using real time PCR on lung swabs, M. ovipneumoniae was detected in all of the 19 pneumonic lungs examined. Gross pathological examination revealed heavy consolidations primarily in the cranial parts of the lungs and it also identified one case of otitis media. Histologically, lung lesions were characterized as acute to subacute mixed exudative and moderately proliferative bronchoalveolar pneumonia. Immunohistochemical (IHC) examination revealed high load of M. ovipneumoniae antigens within lung lesions, with particularly intensive staining in the neutrophils. Similar IHC finding were observed in archived lung tissue blocks from animals examined during the 2006 epidemic. An M. ovipneumoniae specific ELISA was applied on bio-banked muskox sera from stray muskoxen killed in the period 2004-2013 and sick muskoxen culled, as well as sera from wild reindeer (Rangifer tarandus tarandus) on Dovre and muskoxen from Greenland. Serology and mycoplasma culturing was also carried out on sheep that had been on pasture in the muskox area during the outbreak in 2012. Our findings indicated separate introductions of M. ovipneumoniae infection in 2006 and 2012 from infected co-grazing sheep. Salt licks shared by the two species were a

  5. Mycoplasma ovipneumoniae--a primary cause of severe pneumonia epizootics in the Norwegian Muskox (Ovibos moschatus population.

    Directory of Open Access Journals (Sweden)

    Kjell Handeland

    Full Text Available The Norwegian muskox (Ovibos moschatus population lives on the high mountain plateau of Dovre and originates from animals introduced from Greenland. In the late summers of 2006 and 2012, severe outbreaks of pneumonia with mortality rates of 25-30% occurred. During the 2012 epidemic high quality samples from culled sick animals were obtained for microbiological and pathological examinations. High throughput sequencing (pyrosequencing of pneumonic lung tissue revealed high concentrations of Mycoplasma ovipneumoniae in all six animals examined by this method and Pasteurella multocida subsp. multocida in four animals, whereas no virus sequences could be identified. Mycoplasma ovipneumoniae and P. multocida multocida were also isolated by culture. Using real time PCR on lung swabs, M. ovipneumoniae was detected in all of the 19 pneumonic lungs examined. Gross pathological examination revealed heavy consolidations primarily in the cranial parts of the lungs and it also identified one case of otitis media. Histologically, lung lesions were characterized as acute to subacute mixed exudative and moderately proliferative bronchoalveolar pneumonia. Immunohistochemical (IHC examination revealed high load of M. ovipneumoniae antigens within lung lesions, with particularly intensive staining in the neutrophils. Similar IHC finding were observed in archived lung tissue blocks from animals examined during the 2006 epidemic. An M. ovipneumoniae specific ELISA was applied on bio-banked muskox sera from stray muskoxen killed in the period 2004-2013 and sick muskoxen culled, as well as sera from wild reindeer (Rangifer tarandus tarandus on Dovre and muskoxen from Greenland. Serology and mycoplasma culturing was also carried out on sheep that had been on pasture in the muskox area during the outbreak in 2012. Our findings indicated separate introductions of M. ovipneumoniae infection in 2006 and 2012 from infected co-grazing sheep. Salt licks shared by the two

  6. Microfluidic Platform versus Conventional Real-time PCR for the Detection of Mycoplasma pneumoniae in Respiratory Specimens

    Science.gov (United States)

    Wulff-Burchfield, Elizabeth; Schell, Wiley A.; Eckhardt, Allen E.; Pollack, Michael G.; Hua, Zhishan; Rouse, Jeremy L.; Pamula, Vamsee K.; Srinivasan, Vijay; Benton, Jonathan L.; Alexander, Barbara D.; Wilfret, David A.; Kraft, Monica; Cairns, Charles; Perfect, John R.; Mitchell, Thomas G.

    2010-01-01

    Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. PCR has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine out-patient or implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Inc.) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but three times faster and offers an inexpensive and convenient diagnostic test for microbial DNA. PMID:20227222

  7. 肺炎嗜衣原体菌体抗原的制备及应用%Preparation and use of Chlamydophila pneumoniae somatic antigen ELISA

    Institute of Scientific and Technical Information of China (English)

    李雪萍; 陈学勤; 钱利生

    2009-01-01

    目的 制备肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)菌体抗原,纯化后建立ELISA方法,用于血清Cpn抗体的检测. 方法 应用进口Cpn毒株感染Hep-2细胞,利用吖啶橙染色和直接免疫荧光染色判断Cpn感染细胞,制备Cpn抗原.用初步纯化的Cpn抗原建立ELISA方法检测人血清特异性抗体. 结果 吖啶橙染色和直接免疫荧光检测显示,Cpn成功感染Hep-2细胞.提取Cpn抗原,用ELISA检测84份病人血清特异抗体,阳性率为52.4%,ELISA试剂盒法的阳性率为51.2%,差异无统计学意义(P>0.05).用Cpn菌体抗原ELISA检测209份病人血清,阳性率59.3%(其中心血管疾病患者血清138份,阳性率58.0%;呼吸系统疾病患者血清71份,阳性率62.0%);检测84份健康者血清,阳性率2.4%,差异有统计学意义(P<0.05). 结论 Cpn菌体抗原ELISA检测血清抗Cpn抗体可用于判断Cpn感染;病人血清Cpn抗体阳性表明,某些心血管和呼吸系统疾病患者的致病原因可能与肺炎嗜衣原体感染有关.

  8. Antigen-pulsed bone marrow-derived and pulmonary dendritic cells promote Th2 cell responses and immunopathology in lungs during the pathogenesis of murine Mycoplasma pneumonia.

    Science.gov (United States)

    Dobbs, Nicole A; Zhou, Xia; Pulse, Mark; Hodge, Lisa M; Schoeb, Trenton R; Simecka, Jerry W

    2014-08-01

    Mycoplasmas are a common cause of pneumonia in humans and animals, and attempts to create vaccines have not only failed to generate protective host responses, but they have exacerbated the disease. Mycoplasma pulmonis causes a chronic inflammatory lung disease resulting from a persistent infection, similar to other mycoplasma respiratory diseases. Using this model, Th1 subsets promote resistance to mycoplasma disease and infection, whereas Th2 responses contribute to immunopathology. The purpose of the present study was to evaluate the capacity of cytokine-differentiated dendritic cell (DC) populations to influence the generation of protective and/or pathologic immune responses during M. pulmonis respiratory disease in BALB/c mice. We hypothesized that intratracheal inoculation of mycoplasma Ag-pulsed bone marrow-derived DCs could result in the generation of protective T cell responses during mycoplasma infection. However, intratracheal inoculation (priming) of mice with Ag-pulsed DCs resulted in enhanced pathology in the recipient mice when challenged with mycoplasma. Inoculation of immunodeficient SCID mice with Ag-pulsed DCs demonstrated that this effect was dependent on lymphocyte responses. Similar results were observed when mice were primed with Ag-pulsed pulmonary, but not splenic, DCs. Lymphocytes generated in uninfected mice after the transfer of either Ag-pulsed bone marrow-derived DCs or pulmonary DCs were shown to be IL-13(+) Th2 cells, known to be associated with immunopathology. Thus, resident pulmonary DCs most likely promote the development of immunopathology in mycoplasma disease through the generation of mycoplasma-specific Th2 responses. Vaccination strategies that disrupt or bypass this process could potentially result in a more effective vaccination.

  9. Surfactant protein-A suppresses eosinophil-mediated killing of Mycoplasma pneumoniae in allergic lungs.

    Directory of Open Access Journals (Sweden)

    Julie G Ledford

    Full Text Available Surfactant protein-A (SP-A has well-established functions in reducing bacterial and viral infections but its role in chronic lung diseases such as asthma is unclear. Mycoplasma pneumoniae (Mp frequently colonizes the airways of chronic asthmatics and is thought to contribute to exacerbations of asthma. Our lab has previously reported that during Mp infection of non-allergic airways, SP-A aides in maintaining airway homeostasis by inhibiting an overzealous TNF-alpha mediated response and, in allergic mice, SP-A regulates eosinophilic infiltration and inflammation of the airway. In the current study, we used an in vivo model with wild type (WT and SP-A(-/- allergic mice challenged with the model antigen ovalbumin (Ova that were concurrently infected with Mp (Ova+Mp to test the hypothesis that SP-A ameliorates Mp-induced stimulation of eosinophils. Thus, SP-A could protect allergic airways from injury due to release of eosinophil inflammatory products. SP-A deficient mice exhibit significant increases in inflammatory cells, mucus production and lung damage during concurrent allergic airway disease and infection (Ova+Mp as compared to the WT mice of the same treatment group. In contrast, SP-A deficient mice have significantly decreased Mp burden compared to WT mice. The eosinophil specific factor, eosinophil peroxidase (EPO, which has been implicated in pathogen killing and also in epithelial dysfunction due to oxidative damage of resident lung proteins, is enhanced in samples from allergic/infected SP-A(-/- mice as compared to WT mice. In vitro experiments using purified eosinophils and human SP-A suggest that SP-A limits the release of EPO from Mp-stimulated eosinophils thereby reducing their killing capacity. These findings are the first to demonstrate that although SP-A interferes with eosinophil-mediated biologic clearance of Mp by mediating the interaction of Mp with eosinophils, SP-A simultaneously benefits the airway by limiting inflammation

  10. Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey.

    Science.gov (United States)

    Kılıc, Ayşe; Kalender, Hakan; Eroksuz, Hatice; Muz, Adile; Tasdemir, Bülent

    2013-10-01

    This study used cultures, polymerase chain reaction (PCR), and immunoperoxidase to examine samples from 216 lungs from sheep and lambs with macroscopic pneumonia lesions for the presence of Mycoplasma species. DNA was extracted from lung tissue samples and broth cultures with the help of a DNA extraction kit and replicated using genus-specific and species-specific primers for mycoplasma. The lung samples were examined by the immunoperoxidase method using hyperimmune Mycoplasma ovipneumoniae serum. The randomly amplified polymorphic DNA (RAPD) test was used for the molecular typing of M. ovipneumoniae isolates. Mycoplasma was isolated in the cultures of 80 (37.03 %) of a total of 216 lung samples. Genus-specific mycoplasma DNA was identified by PCR in 96 (44.44 %) samples in broth cultures and 36 (16.66 %) directly in the lung tissue. Of these 96 cases in which genus-specific identification was made, 57 (59.37 %) were positive for reaction with species-specific primers for M. ovipneumoniae and 31 (32.29 %) for Mycoplasma arginini. The DNA of neither of the latter two species could be identified in the remaining eight samples (8.33 %) where mycoplasma had been identified. As for the immunoperoxidase method, it identified M. ovipneumoniae in 61 of 216 lung samples (28 %). Positive staining was concentrated in the bronchial epithelium cell cytoplasm and cell surface. RAPD analysis resulted in 15 different profiles. Our results suggest that PCR methods could be successfully used in the diagnosis of mycoplasma infections as an alternative to culture method and identifying this agent at the species level.

  11. Interactions of surface-displayed glycolytic enzymes of Mycoplasma pneumoniae with components of the human extracellular matrix.

    Science.gov (United States)

    Gründel, Anne; Jacobs, Enno; Dumke, Roger

    2016-12-01

    Mycoplasma pneumoniae is a major cause of community-acquired respiratory infections worldwide. Due to the strongly reduced genome, the number of virulence factors expressed by this cell wall-less pathogen is limited. To further understand the processes during host colonization, we investigated the interactions of the previously confirmed surface-located glycolytic enzymes of M. pneumoniae (pyruvate dehydrogenase A-C [PdhA-C], glyceraldehyde-3-phosphate dehydrogenase [GapA], lactate dehydrogenase [Ldh], phosphoglycerate mutase [Pgm], pyruvate kinase [Pyk] and transketolase [Tkt]) to the human extracellular matrix (ECM) proteins fibrinogen (Fn), fibronectin (Fc), lactoferrin (Lf), laminin (Ln) and vitronectin (Vc), respectively. Concentration-dependent interactions between Fn and Vc and all eight recombinant proteins derived from glycolytic enzymes, between Ln and PdhB-C, GapA, Ldh, Pgm, Pyk and Tkt, between Lf and PdhA-C, GapA and Pyk, and between Fc and PdhC and GapA were demonstrated. In most cases, these associations are significantly influenced by ionic forces and by polyclonal sera against recombinant proteins. In immunoblotting, the complex of human plasminogen, activator (tissue-type or urokinase plasminogen activator) and glycolytic enzyme was not able to degrade Fc, Lf and Ln, respectively. In contrast, degradation of Vc was confirmed in the presence of all eight enzymes tested. Our data suggest that the multifaceted associations of surface-localized glycolytic enzymes play a potential role in the adhesion and invasion processes during infection of human respiratory mucosa by M. pneumoniae.

  12. A single tube modified allele-specific-PCR for rapid detection of erythromycin-resistant Mycoplasma pneumoniae in Beijing

    Institute of Scientific and Technical Information of China (English)

    LI Shao-li; SUN Hong-mei; ZHAO Han-qing; CAO Ling; YUAN Yi; FENG Yan-ling; XUE Guan-hua

    2012-01-01

    Background Mycoplasma pneumoniae (M.pneumoniae) is one of the common pathogens causing atypical pneumonia.In recent years,resistance to macrolides has become more common,especially in China.Previous studies have confirmed that the mutation at position 2063 in domain V of the 23S rRNA is the most prevalent,followed by the mutation at position 2064.Reported molecular detection methods for the identification of these mutations include direct sequencing,restriction fragment length polymorphism analysis,real-time polymerase chain reaction (PCR) with high-resolution melt analysis,and nested PCR-linked with capillary electrophoresis,etc.The most commonly used method for monitoring resistance-conferring mutations in M.pneumoniae is direct DNA sequencing of PCR or nested PCR products.However,these methods are time-consuming,labor-intensive or need expensive equipments.Therefore the development of rapid and sensitive methods is very important for monitoring the resistance globally.Methods In this study,we reported a fast and cost-effective method for detecting 2063 and/or 2064 macrolide resistant mutations from specimens using a modified allele-specific PCR analysis,and all results were compared with the sequencing data.We also analyzed the clinical courses of these samples to confirm the modified allele-specific PCR results.Results Among 97 M.pneumoniae specimens,88 were found to possess mutations by this method,and all modified allele-specific PCR analysis results were consistent with the sequencing data.The data of the clinical courses of these 97cases showed that they suffered from severe pneumonia.Erythromycin showed better efficacy on cases from which no macrolide resistance mutation was found on their specimens.However,in some cases from which mutations were detected,erythromycin monotherapy had poor efficacy,and on these patients severe symptoms improved only when azithromycin was added to the treatment.Conclusions The drug-resistant M.pneumoniae is very common in

  13. 成人肺炎支原体感染的临床分析%Result Analysis of adult Mycoplasma Pneumoniae Pneumonia

    Institute of Scientific and Technical Information of China (English)

    刘鸿梅; 任春阳

    2013-01-01

    Objective:To analysis the clinical infection and drug resistance characteristics in isolates of Mycoplasma Pneumoniae(MP), and provide evidence for clinical therapy.Methods:Col ecting 1288 throat swabs of patients with acute respiratory tract infection to detecting Mycoplasma Pneumoniae by Rapid Identification Culturing And Chemosensitivity Kit.Results:255 strains of MP were isolated from 1288 cases,the positive rate was 19.79%. MP had the higher susceptible rate to azithromycin, lavo-ofloxacin, galtixacin, sparfloxacin and the highest resistance rate to erythrocin. Conclusions:Azithromycin can be used as the first-choice drugs in MP treatment.%目的:了解我院成人肺炎支原体(MP)感染及耐药特征,为临床合理使用抗生素提供依据。方法:采用MP快速鉴定培养药敏试剂盒对1288例急性呼吸道感染的患者咽拭子标本进行MP快速鉴定培养与药敏试验。结果:MP培养共有255例阳性,阳性率19.79%;MP对阿奇霉素、左氧氟沙星、加替沙星和司帕沙星的敏感率高;对红霉素的耐药率最高。结论:临床治疗MP应该根据药敏试验结果选用药物,其耐药性不容忽视,阿奇霉素是较理想选择。

  14. 小儿支原体肺炎42例临床分析%The Clinical Analysis of 42 Cases with Mycoplasma Pneumoniae Pneumonia in Children

    Institute of Scientific and Technical Information of China (English)

    钟昌课

    2013-01-01

    Objective:To explore the clinical characteristics,therapeutic methods and curative effect of mycoplasma pneumoniae pneumonia in children.Method:To review and analyse the clinical data of 42 cases with mycoplasma pneumoniae pneumonia in children from December 2011 to October 2012 in our hospital.The patients were diagnosed through pathogens,MP-IgM in serum and imageological examination.The clinical characteristics were summarized 21 cases were chosen as treatment group which treated with sensitive antibiotics by using the disc diffusion method to test the resistance of common antibiotics of mycoplasma pneumoniae on the basis of conventional treatments.The patients treated with conventional treatments were chosen as the control group.Result:The common clinical feature were cough and fever,the CRP was significantly higher,this feature suggested there were virus or bacterial infection in mycoplasma pneumoniae.The total efficiency in treatment group was higher than which in control group(P<0.05).The time of cough disappear,continue fever,asthma disappear and hospitalization in treatment group were shorter which in control group(P<0.05).The bronchoscopy with bronchoalveolar lavage in the treatment of refractory mycoplasmal pneumonia in children had better effect.Conclusion:The mycoplasma pneumoniae uaually combines with bacterial infection.The pulmonary lesions is the main feature in infant,while it isn’t the main feature in children.The treatment according to sensitive antibiotics by the drug sensitive test can improve the efficacy.%  目的:探讨小儿支原体肺炎的临床特点、治疗方法及疗效。方法:回顾性分析笔者所在医院2011年12月-2012年10月收治的42例支原体肺炎患儿的临床资料,该组患儿均经过病原菌、血清MP-IgM检测、影像学检查确诊,总结该组患儿的临床特点。其中21例患儿在常规治疗的基础上采用纸片扩散法检测肺炎支原体对常用抗生素的耐药性,选

  15. 儿童支原体肺炎临床分析%Mycoplasma pneumoniae pneumonia in children

    Institute of Scientific and Technical Information of China (English)

    马向萍; 于晓磊; 蒋少华; 王军杰

    2015-01-01

    目的:了解本地区儿童支原体肺炎的临床特点。方法:对住院治疗的347例儿童支原体肺炎患儿临床特征进行回顾性分析。结果:1.347例儿童支原体肺炎患儿以1—7岁所占比例最高,占82.13%。2.婴幼儿组冬春季发病率高于少儿组,差异有统计学意义(P<0.05)。性别、民族比较差异无统计学意义(P﹥0.05)。3.少儿组高热及热程﹥4天比婴幼儿组显著升高,婴幼儿组全身症状显著高于少儿组,差异有统计学意义(P<0.05)。(4)体征:所有患儿都有呼吸增快,听诊呼吸音粗糙,婴幼儿组患儿肺部啰音较少儿组患儿明显升高,差异有统计学意义(P<0.05)。(5)实验室检查:病例组中性粒细胞比例、I g A、C3及C4显著低于对照组,病例组白细胞计数、淋巴细胞比例及C K-M B显著高于对照组,两组比较差异均有统计学意义(P<0.05)。病例组单核细胞比例、嗜酸粒细胞比例、血小板计数、CRP、血沉、IgG及IgM与对照组比较差异无统计学意义(P﹥0.05)。(6)X线检查:少儿组肺段实质浸润、肺纹理增粗及肺门影增浓显著高于婴幼儿组,两组比较差异均有统计学意义(P<0.05)。结论:本院儿童支原体肺炎的临床特点主要以1—7岁的患儿发病为主,好发于冬春季。发热、咳嗽以及全身症状为主要症状,婴幼儿肺部体征重,常累及全身各系统,少儿组多出现高热且发热时间长。实验室检查白细胞计数、淋巴细胞比例及CK-MB升高,中性粒细胞比例、IgA、C3及C4测下降;影像学检查以少儿组严重,主要表现为肺段实质浸润、肺纹理增粗及肺门影增浓。%Objecfive To explore the clinical features of MPP in children in this region.Methods Of 347 cases of children hospitalized with MPP in children with clinical features were retrospectively analyzed.Results 1.347 cases of children with mycoplasma pneumonia

  16. Chronic non-progressive pneumonia of sheep in New Zealand - a review of the role of Mycoplasma ovipneumoniae.

    Science.gov (United States)

    Alley, M R; Ionas, G; Clarke, J K

    1999-10-01

    Chronic non-progressive pneumonia (CNP) is a common disease which affects lambs in New Zealand during late summer and autumn. Mycoplasma ovipneumoniae can be recovered from a high proportion of lesions but it is also present in some normal lungs. Bacteria, especially Pasteurella haemolytica, can also be recovered from more than half the lungs of affected animals. Isolates of M. ovipneumoniae are genetically heterogeneous, as demonstrated by examination of their DNA or total cellular proteins, and are serologically heterogeneous as shown by metabolic inhibition tests. The number of strains present in New Zealand is large and several distinguishable strains can be recovered from each affected lung. Mycoplasma ovipneumoniae has pathogenic potential as indicated by its ability to produce hydrogen peroxide, cause ciliostasis and by its possession of a capsule. Chronic non-progressive pneumonia can be transmitted consistently to over 50% of lambs by inoculation of pooled pneumonic lung homogenate and transmission can be suppressed by broad spectrum antibiotics. In contrast, penicillin does not prevent the development of lesions but diminishes their severity. Pooled lung homogenate treated with digitonin, which inactivates mycoplasmas, has failed to transmit CNP. Pure cultures of M. ovipneumoniae produce only mild lesions in some animals, whereas inoculation with pooled lung homogenate (from which no viruses were isolated) containing mixed strains of M. ovipneumoniae and free from bacteria, is more effective in producing lesions. Research work to date suggests that CNP may be initiated by colonisation of the lung by M. ovipneumoniae which causes ciliostasis and elicits an exudate allowing colonisation of the lungs by bacteria especially M. haemolytica and by other strains of M. ovipneumoniae. The immune response to the initial strain of M. ovipneumoniae may inhibit its replication but would be less effective in inhibiting heterologous strains of the organism allowing

  17. Prevalência de Chlamydia Pneumoniae e Mycoplasma Pneumoniae em diferentes formas da doença coronariana Prevalencia de Chlamydia Pneumoniae y Mycoplasma Pneumoniae en diferentes formas de la enfermedad coronaria Prevalence of Chlamydia Pneumoniae and Mycoplasma Pneumoniae in different forms of coronary disease

    Directory of Open Access Journals (Sweden)

    Irineu Luiz Maia

    2009-06-01

    Full Text Available FUNDAMENTO: Vários agentes infecciosos foram investigados desde que se demonstrou a associação entre infecção e aterosclerose, porém os resultados desses estudos são conflitantes. OBJETIVO: Testar a associação entre títulos séricos de anticorpos anti-Chlamydia e anti-Mycoplasma em diferentes formas de síndromes coronarianas agudas (SCA. MÉTODOS: Cento e vinte e seis pacientes foram divididos em quatro grupos: SCA com elevação do segmento ST (32 pacientes, SCA sem elevação do segmento ST (30 pacientes, doença arterial coronariana crônica (30 pacientes e doadores de sangue sem doença coronariana conhecida (34 pacientes - grupo-controle. Nos primeiros dois grupos, amostras de soro foram coletadas na admissão (primeiras 24 horas de hospitalização e após 6 meses de seguimento. Nos outros dois grupos, colheu-se apenas uma amostra basal. Em todas as amostras, anticorpos IgG anti-Chlamydia e anti-Mycoplasma foram dosados por imunofluorescência indireta. RESULTADOS: Diferenças significativas foram observadas entre a medida basal e após 6 meses de seguimento nos pacientes com infarto do miocárdio com elevação do segmento ST, tanto para Chlamydia (650±115,7 vs. 307±47,5, p = 0,0001 quanto para Mycoplasma (36,5±5,0 vs. 21,5±3,5, p = 0,0004. Os grupos com SCA tiveram níveis séricos de anticorpos anti-Chlamydia e anti-Mycoplasma mais altos na dosagem basal, em relação aos pacientes com doença arterial coronariana crônica e grupo-controle, mas as diferenças obtidas não tiveram significância estatística. CONCLUSÃO: O presente estudo mostrou associação entre os títulos de anticorpos anti-Chlamydia e anti-Mycoplasma na fase aguda dos pacientes com angina instável ou infarto do miocárdio.FUNDAMENTO: Se han investigado diversos agentes infecciosos desde que se evidenció la asociación entre infección y aterosclerosis, sin embargo esos estudios ofrecen resultados conflictivos. OBJETIVO: Probar la asociación entre

  18. Clinical and economic burden of community-acquired pneumonia amongst adults in the Asia-Pacific region.

    Science.gov (United States)

    Song, Jae-Hoon; Thamlikitkul, Visanu; Hsueh, Po-Ren

    2011-08-01

    Community-acquired pneumonia (CAP) is an important cause of mortality and morbidity amongst adults in the Asia-Pacific region. Literature published between 1990 and May 2010 on the clinical and economic burden of CAP amongst adults in this region was reviewed. CAP is a significant health burden with significant economic impact in this region. Chronic obstructive pulmonary disease, cardiovascular disease, diabetes mellitus and advanced age were risk factors for CAP. Aetiological agents included Streptococcus pneumoniae, Klebsiella pneumoniae, Gram-negative bacteria, Mycobacterium tuberculosis, Burkholderia pseudomallei, Staphylococcus aureus and atypical pathogens (Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella spp.), with important differences in the prevalence of these pathogens within the region. Antibiotic resistance was significant but was not linked to excess mortality. Aetiological pathogens remained susceptible to newer antimicrobial agents. Rational antibiotic use is essential for preventing resistance, and increased surveillance is required to identify future trends in incidence and aetiology and to drive treatment and prevention strategies.

  19. 小儿荨麻疹与肺炎支原体相关性探讨%Study on the correlation between urticaria and mycoplasma pneumonia in children

    Institute of Scientific and Technical Information of China (English)

    梁正

    2016-01-01

    目的:探讨小儿荨麻疹与肺炎支原体的相关性。方法:收治荨麻疹患者76例,其中67例与感染有关,对其临床资料进行分析。结果:67例患儿中,33例(49.2%)肺炎支原体抗体和呼吸道病原体十一项肺炎支原体抗体(IgM)阳性。经治疗后,皮疹均在1~8 d内消失。结论:临床工作中,遇到荨麻疹患儿时,应考虑肺炎支原体感染因素,避免漏诊和误诊。%Objective:To explore the correlation between urticaria and mycoplasma pneumonia in children.Methods:76 patients with urticaria were selected.Among them,67 cases had infection.The clinical data were analyzed.Results:In 67 cases,33 cases(49.2%) were positive in mycoplasma pneumoniae antibody and respiratory tract pathogens eleven mycoplasma pneumoniae antibodies(IgM).After the treatment,the rash was disappeared in 1 to 8 days.Conclusion:In clinical work,in the face of urticaria,we should consider the mycoplasma pneumoniae infection,so as to avoid missed diagnosis and misdiagnosis.

  20. Effect of azithromycin, montelukast combined with pulmicort respulas therapy on the degree of inflammation and lung function in children with mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    Jian-mei Zhang; Jin-fang Zhou; Min Wang; Jue Bai; Yan Jia

    2016-01-01

    Objective:To analyze the effect of azithromycin, montelukast combined with pulmicort respulas therapy on the degree of inflammation and lung function in children with mycoplasma pneumonia.Methods:A total of 318 cases of children with mycoplasma pneumonia treated in our hospital from February 2013 to February 2016 were randomly divided into observation group and control group (n=159). Control group received azithromycin and montelukast therapy, observation group received azithromycin, montelukast combined with pulmicort respulas therapy, and then the degree of inflammation, lung function, etc. were compared between two groups.Results:V-T, t-PTEF/t-E, TEF25/PTEF, FEV1, FVC, FEV1/FVC and MVV values of observation group after treatment were higher than those of control groupand differences in MTIF/MTEF values were not statistically significant between groups; serum CCSP value was higher than that of control group, and IL-17, MCP-4, MDC and CysLTs values were lower than those of control group.Conclusion:Azithromycin, montelukast combined with pulmicort respulas therapy can reduce the systemic inflammatory state and optimize lung function in children with mycoplasma pneumonia, and it has positive clinical significance.

  1. 中药方剂治疗猪支原体肺炎的效果%Effect of Chinese Herbs on Mycoplasma Pneumonia of Swine

    Institute of Scientific and Technical Information of China (English)

    李国旺; 赵恒章; 苗志国

    2011-01-01

    为给中药防治猪支原体肺炎提供依据,进行了中药方剂(苏子、款冬花、杏仁、桔梗、陈皮、甘草、鱼腥草等)治疗猪支原体肺炎的效果试验,结果表明:中药方剂的治愈率、总显效率分别为73%和87%,高于西药制剂盐酸林可霉素注射液和泰乐菌素注射液的治愈率和总显效率.同时中药方剂对猪的日增重有一定的促进作用.%Effect of Chinese herbs on mycoplasma pneumonia of swine was studied to provide reference for control of mycoplasma pneumonia of swine using Chinese herbs(perillaseed, tussilago farfara flower,almond, platycodon root, orange peel, licorice root, cordate houttuynia, et al). Results showed that the cure rate and total effectual rate of Chinese herbs on mycoplasma pneumonia of swine were 73 % and 87 % respectively, which were higher than that of lincomycin hydrochloride injection and tylosin injection. And Chinese herbs had certain promoting effect on daily weight increment of swine as well.

  2. Subunits of the Pyruvate Dehydrogenase Cluster of Mycoplasma pneumoniae Are Surface-Displayed Proteins that Bind and Activate Human Plasminogen.

    Directory of Open Access Journals (Sweden)

    Anne Gründel

    Full Text Available The dual role of glycolytic enzymes in cytosol-located metabolic processes and in cell surface-mediated functions with an influence on virulence is described for various micro-organisms. Cell wall-less bacteria of the class Mollicutes including the common human pathogen Mycoplasma pneumoniae possess a reduced genome limiting the repertoire of virulence factors and metabolic pathways. After the initial contact of bacteria with cells of the respiratory epithelium via a specialized complex of adhesins and release of cell-damaging factors, surface-displayed glycolytic enzymes may facilitate the further interaction between host and microbe. In this study, we described detection of the four subunits of pyruvate dehydrogenase complex (PDHA-D among the cytosolic and membrane-associated proteins of M. pneumoniae. Subunits of PDH were cloned, expressed and purified to produce specific polyclonal guinea pig antisera. Using colony blotting, fractionation of total proteins and immunofluorescence experiments, the surface localization of PDHA-C was demonstrated. All recombinant PDH subunits are able to bind to HeLa cells and human plasminogen. These interactions can be specifically blocked by the corresponding polyclonal antisera. In addition, an influence of ionic interactions on PDHC-binding to plasminogen as well as of lysine residues on the association of PDHA-D with plasminogen was confirmed. The PDHB subunit was shown to activate plasminogen and the PDHB-plasminogen complex induces degradation of human fibrinogen. Hence, our data indicate that the surface-associated PDH subunits might play a role in the pathogenesis of M. pneumoniae infections by interaction with human plasminogen.

  3. A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression.

    Directory of Open Access Journals (Sweden)

    Sebastian R Schmidl

    2011-09-01

    Full Text Available Mycoplasma pneumoniae is a causative agent of atypical pneumonia. The formation of hydrogen peroxide, a product of glycerol metabolism, is essential for host cell cytotoxicity. Phosphatidylcholine is the major carbon source available on lung epithelia, and its utilization requires the cleavage of deacylated phospholipids to glycerol-3-phosphate and choline. M. pneumoniae possesses two potential glycerophosphodiesterases, MPN420 (GlpQ and MPN566. In this work, the function of these proteins was analyzed by biochemical, genetic, and physiological studies. The results indicate that only GlpQ is an active glycerophosphodiesterase. MPN566 has no enzymatic activity as glycerophosphodiesterase and the inactivation of the gene did not result in any detectable phenotype. Inactivation of the glpQ gene resulted in reduced growth in medium with glucose as the carbon source, in loss of hydrogen peroxide production when phosphatidylcholine was present, and in a complete loss of cytotoxicity towards HeLa cells. All these phenotypes were reverted upon complementation of the mutant. Moreover, the glpQ mutant strain exhibited a reduced gliding velocity. A comparison of the proteomes of the wild type strain and the glpQ mutant revealed that this enzyme is also implicated in the control of gene expression. Several proteins were present in higher or lower amounts in the mutant. This apparent regulation by GlpQ is exerted at the level of transcription as determined by mRNA slot blot analyses. All genes subject to GlpQ-dependent control have a conserved potential cis-acting element upstream of the coding region. This element overlaps the promoter in the case of the genes that are repressed in a GlpQ-dependent manner and it is located upstream of the promoter for GlpQ-activated genes. We may suggest that GlpQ acts as a trigger enzyme that measures the availability of its product glycerol-3-phosphate and uses this information to differentially control gene expression.

  4. Application of nucleic acid amplification technique for diagnosis of Chlamydophila pneumonia infection%核酸扩增技术在肺炎嗜衣原体感染诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    张军华; 陈丽丽; 吴移谋

    2010-01-01

    Chlamydia pneumoniae (Cpn) is a kind of microorganism parasitizing in eukaryotic cells, causing human respiratory tract infection, and having persistent infection in the body. Rapid diagnosis in the early stage of Cpn infection helps to prevent the spread of disease and the formation of complications. On the Cpn diagnostic methods, domestic and foreign scholars have carried out a series of studys and made great progress. This paper reviews the application of nucleic acid amplification technique for the diagnosis of Cpn infection.%肺炎嗜衣原体(Chlamydophila pneumonia,Cpn)是一类引起人类呼吸道感染的、专营真核细胞内寄生生活的微生物,在人体中存在持续感染.Cpn感染早期的快速诊断有利于阻止疾病的传播和防止并发症的形成.有关Cpn的诊断方法,国内外学者进行了一系列研究并取得了很大的进展.本文就核酸扩增技术在诊断Cpn感染中的应用作一综述.

  5. 儿童肺炎支原体感染性大叶性肺炎86例临床分析%Clinical Study of Mycoplasma Pneumonia Infection of Lobor Pneumonia in 86 Children

    Institute of Scientific and Technical Information of China (English)

    高兰平; 唐进; 陈炜

    2011-01-01

    Objective To explore the clinical characteristics of mycoplasma pneumonia infection of lobar pneumonia. Methods The clinical data of 86 mycoplasma pneumonia infection of lobar pneumonia that were diagnosed and treated at our hospital from January 2007 to December 2010 were analysed. Results The clinical characters of 86 lobar pneumonia mycoplasma pneumonia are different from those of before: majority of patients were children being student,the incidense of infants is increasing,the process was long, the samptoms of respiratory was tipical, the continuous fever was few, the physical sign may be not typical, the injury to lung was more and typical, the β-lactants antibiotic was not effective, but macrolides was effective. Conclusions Clinical characters of MP pneumonia are needed to master, children lobar pneumonia should pay attention to mycoplasma pneumonia infection, if necessary, should adopt macrolides in earlier period.%目的 探讨肺炎支原体(MP)感染性大叶性肺炎临床特征.方法 对我院收住的86例支原体感染性大叶性肺炎病例进行分析.结果 86例支原体感染性大叶性肺炎有以下特点:以学龄期儿童多见,婴幼儿发病有增加趋势,病程长,呼吸道症状突出,稽留热少见,体征不典型,肺外损害多且突出,β-内酰胺类抗生素治疗无效,大环内酯类治疗有效.结论 需掌握MP肺炎的临床特征,以大叶性肺炎为主要改变者应警惕肺炎支原体感染的可能,必要时早期应用大环内酯类药物.

  6. Specific multilocus variable-number tandem-repeat analysis genotypes of Mycoplasma pneumoniae are associated with diseases severity and macrolide susceptibility.

    Directory of Open Access Journals (Sweden)

    Jiuxin Qu

    Full Text Available Clinical relevance of multilocus variable-number tandem-repeat (VNTR analysis (MLVA in patients with community-acquired pneumonia (CAP by Mycoplasma pneumoniae (M. pneumoniae is unknown. A multi-center, prospective study was conducted from November 2010 to April 2012. Nine hundred and fifty-four CAP patients were consecutively enrolled. M. pneumoniae clinical isolates were obtained from throat swabs. MLVA typing was applied to all isolates. Comparison of pneumonia severity index (PSI and clinical features among patients infected with different MLVA types of M. pneumoniae were conducted. One hundred and thirty-six patients were positive with M. pneumoniae culture. The clinical isolates were clustered into 18 MLVA types. One hundred and fourteen (88.3% isolates were resistant to macrolide, covering major MLVA types. The macrolide non-resistant rate of M. pneumoniae isolates with Mpn13-14-15-16 profile of 3-5-6-2 was significantly higher than that of other types (p ≤ 0.001. Patients infected with types U (5-4-5-7-2 and J (3-4-5-7-2 had significantly higher PSI scores (p<0.001 and longer total duration of cough (p = 0.011. Therefore it seems that there is a correlation between certain MLVA types and clinical severity of disease and the presence of macrolide resistance.

  7. Treatment Management Experience of Pediatric Mycoplasma Pneumoniae Pneumonia with Diarrhea%小儿肺炎支原体肺炎合并腹泻的检验管理体会

    Institute of Scientific and Technical Information of China (English)

    乔献伟

    2015-01-01

    目的:探讨小儿肺炎支原体肺炎合并腹泻治疗管理体会。方法选取334例南阳市第一人民医院于2012年6月—2013年12月收治的肺炎支原体肺炎患儿为对象,将所有患儿按年龄分为婴幼儿组(3月≤年龄<3岁,共185例)、学龄前组(3岁≤年龄<7岁,共81例)及学龄组(7岁≤年龄<13岁,共76例),回顾性分析3组患儿病例资料与病原体检查结果。结果3组患儿中合并腹泻143例,分别为中婴幼儿组89例,学龄前组29例,学龄组25例,婴幼儿组合并腹泻患儿最多,与用药治疗前比较,治疗后合并腹泻患儿例数明显增加,P<0.05;病原体检查结果显示,用药前引起腹泻的主要致病原为轮状病毒和细菌;用药后,患儿合并腹泻的致病原主要是真菌。结论治疗小儿支原体肺炎要综合考虑腹泻原因,合理使用抗生素可降低合并腹泻等发生率。%Objective To discuss the treatment management experience of pediatric mycoplasma Pneumoniae pneumonia with diarrhea.Methods 334 cases with mycoplasma Pneumoniae pneumonia in The First People's Hospital of Nanyang city from 2012.06 to 2013.12, They were divided into three groups : infants' group (3 month age < 3 year-old, 185 cases), preschool group(3 age < 7 years old, 81 cases) and school age group(7 age < 13 years old,76 cases ). Retrospective analy-sis the clinical data of three groups and pathogenic examination result. Results There were 143 cases pediatric mycoplasma Pneumoniae pneumonia with diarrhea in all cases, and 89 cases in infants' group,29 cases in preschool group and 25 cases in school age group ,The number of pediatric mycoplasma pneumoniae pneumonia with diarrhea in infants'?group signifi-cantly higher than the other two groups, significant difference(P<0.05). pathogenic examination result shown that before drug treatment, Rotavirus and bacteria were major pathogen for pediatric mycoplasma Pneumoniae pneumonia with diarrhea , af-ter bdrug treatment, fungus

  8. 分析小儿重症肺炎支原体肺炎的相关因素%Analysis of Factors Associated with Children with Severe Pneumonia Mycoplasma Pneumonia

    Institute of Scientific and Technical Information of China (English)

    缑梦帆

    2015-01-01

    To explore the related factors of children with severe pneumonia mycoplasma pneumonia and its therapeutic effect. Methods:our hospital in March 2013 - June 2014 were analyzed in 40 cases of children with severe pneumonia mycoplasma pneu-monia cases as the research object, for the treatment of children with effect were retrospectively analyzed. Results: through the analysis concluded that children with severe mycoplasma pneumonia patients and correlated with age, pathological changes of the ESR and CRP, statistically significant difference (P < 0. 05). After treatment, the treatment of this group of 40 cases effective rate was 97. 8%. Con-clusion:the different pathological types and infantile pneumonia with patient's age, basic diseases, such as has certain relevance, timely diagnosis can improve therapeutic effect.%目的:探究小儿重症肺炎支原体肺炎的相关因素及治疗效果. 方法:选取我院2013年3月~2014年6月收治的40例小儿重症肺炎支原体肺炎病例为研究对象,为患儿的治疗效果进行回顾性分析. 结果:通过分析得出,患儿重症支原体肺炎患者与年龄、ESR、CRP病变部位等有相关性,差异具有统计学意义(P<0. 05).经过治疗,本组40例患儿的治疗有效率为97. 8%.结论:不同的病理类型及小儿肺炎与患者的年龄、基础疾病等有一定的相关性,及时的确诊可以提升治疗效果.

  9. Comparative "-omics" in mycoplasma pneumoniae clinical isolates reveals key virulence factors

    OpenAIRE

    Maria Lluch-Senar; Luca Cozzuto; Jaime Cano; Javier Delgado; Verónica Llórens-Rico; Sabine Pereyre; Cécile Bebear; Luis Serrano

    2015-01-01

    The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2), differing in the sequence of the P1 adhesin gene. Here, we have sequenced the genomes of 23 clinical isolates of M. pneumoniae. Studying SNPs, non-synonymous mutations, indels and genome rearrangements of these 23 strains and 4 previously sequenced ones, has revealed new subclasses in the t...

  10. 小儿肺炎支原体肺炎合并腹泻的临床检验分析%Clinical inspection analysis of pediatric mycoplasma pneumoniae pneumonia complicated diarrhea

    Institute of Scientific and Technical Information of China (English)

    幸红军

    2015-01-01

    目的:分析小儿肺炎支原体肺炎合并腹泻的临床检验结果。方法:2012年8月-2013年6月收治肺炎支原体肺炎患儿358例,按年龄分为3组。检测3组患儿肺炎支原体IgM抗体、轮状病毒抗原及其他病原菌,分析腹泻发生情况及病原体分布。结果:358例肺炎支原体肺炎患儿合并腹泻150例(41.9%),其中婴幼儿组腹泻发生率22.9%,明显高于学龄前组及学龄组,差异比较有统计学意义(P<0.05)。所选患儿腹泻多见于细菌性肠炎(45.3%),其次为轮状病毒肠炎。婴幼儿组轮状病毒肠炎腹泻46.3%,明显高于其他类型腹泻,差异比较有统计学意义(P<0.05)。结论:肺炎支原体肺炎可引起小儿腹泻,且多见于婴幼儿,在重视患儿粪便细菌培养的同时应加强轮状病毒的检测,防止肺炎支原体肺炎合并腹泻发生。%Objective:To analyze the clinical inspection results of pediatric mycoplasma pneumoniae pneumonia complicated diarrhea.Methods:358 cases of children with mycoplasma pneumoniae pneumonia from August 2012 to June 2013 were divided into three groups according to the age.Mycoplasma pneumoniae IgM antibody,rotavirus antigen and other pathogens of the three groups were detected and the diarrhea occurrence and distribution of pathogens were analyzed.Results:150 cases of children(41.9%) were combined with diarrhea in 358 cases with mycoplasma pneumoniae pneumonia,and the diarrhea incidence of the infant group was 22.9% ,which was significantly higher than that of the preschool group and school-age group,and the difference were statistically significant(P<0.05).The bacterial enteritis were the most among selected children with diarrhea(45.3% ),followed by rotavirus enteritis.The rotavirus enteritis in infant group with diarrhea was 46.3% ,which was significantly higher than other types of diarrhea,and the difference was statistical significant(P<0.05).Conclusion:Mycoplasma pneumoniae pneumonia

  11. The Spread of Mycoplasma pneumoniae Is Polyclonal in Both an Endemic Setting in France and in an Epidemic Setting in Israel

    Science.gov (United States)

    Pereyre, Sabine; Charron, Alain; Hidalgo-Grass, Carlos; Touati, Arabella; Moses, Allon E.; Nir-Paz, Ran; Bébéar, Cécile

    2012-01-01

    Mycoplasma pneumoniae infections occur both endemically and epidemically, and macrolide resistance has been spreading for 10 years worldwide. A substantial increased incidence of M. pneumoniae infections has been reported in several countries since 2010. Whether this increased incidence is attributed to different or to the same M. pneumoniae genotype is unknown. We have developed a multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) for the molecular typing of M. pneumoniae isolates. In this study, the MLVA typing method was modified and validated to be applicable directly to respiratory tract specimens without culture. This method was applied to 34 M. pneumoniae-positive specimens received at the Bordeaux Hospital, France, between 2007 and 2010 in an endemic setting, and to 63 M. pneumoniae-positive specimens collected during an epidemic surge of M. pneumoniae infections in 2010 in Jerusalem, Israel. The M. pneumoniae endemic spread was shown to be polyclonal in France, with 15 MLVA types identified. Strikingly, the Israeli epidemic surge was also a multi-clonal phenomenon, with 18 circulating MLVA types. The macrolide resistance-associated substitution, A2058G, was found in 22% of the Israeli patients. Macrolide-resistant M. pneumoniae belonged to four MLVA types, the MLVA type Z being the most frequent one. An association between the MLVA type Z and macrolide resistance might exist since macrolide resistance was present or generated during the course of illness in all patients infected with this MLVA type. In conclusion, the discriminatory power of the MLVA showed that the spread of M. pneumoniae strains in France in an endemic setting was polyclonal as well as the surge of M. pneumoniae infections in Israel in 2010. PMID:22701675

  12. The spread of Mycoplasma pneumoniae is polyclonal in both an endemic setting in France and in an epidemic setting in Israel.

    Directory of Open Access Journals (Sweden)

    Sabine Pereyre

    Full Text Available Mycoplasma pneumoniae infections occur both endemically and epidemically, and macrolide resistance has been spreading for 10 years worldwide. A substantial increased incidence of M. pneumoniae infections has been reported in several countries since 2010. Whether this increased incidence is attributed to different or to the same M. pneumoniae genotype is unknown. We have developed a multilocus variable-number tandem-repeat (VNTR analysis (MLVA for the molecular typing of M. pneumoniae isolates. In this study, the MLVA typing method was modified and validated to be applicable directly to respiratory tract specimens without culture. This method was applied to 34 M. pneumoniae-positive specimens received at the Bordeaux Hospital, France, between 2007 and 2010 in an endemic setting, and to 63 M. pneumoniae-positive specimens collected during an epidemic surge of M. pneumoniae infections in 2010 in Jerusalem, Israel. The M. pneumoniae endemic spread was shown to be polyclonal in France, with 15 MLVA types identified. Strikingly, the Israeli epidemic surge was also a multi-clonal phenomenon, with 18 circulating MLVA types. The macrolide resistance-associated substitution, A2058G, was found in 22% of the Israeli patients. Macrolide-resistant M. pneumoniae belonged to four MLVA types, the MLVA type Z being the most frequent one. An association between the MLVA type Z and macrolide resistance might exist since macrolide resistance was present or generated during the course of illness in all patients infected with this MLVA type. In conclusion, the discriminatory power of the MLVA showed that the spread of M. pneumoniae strains in France in an endemic setting was polyclonal as well as the surge of M. pneumoniae infections in Israel in 2010.

  13. Animal model of Mycoplasma fermentans respiratory infection

    Directory of Open Access Journals (Sweden)

    Yáñez Antonio

    2013-01-01

    Full Text Available Abstract Background Mycoplasma fermentans has been associated with respiratory, genitourinary tract infections and rheumatoid diseases but its role as pathogen is controversial. The purpose of this study was to probe that Mycoplasma fermentans is able to produce respiratory tract infection and migrate to several organs on an experimental infection model in hamsters. One hundred and twenty six hamsters were divided in six groups (A-F of 21 hamsters each. Animals of groups A, B, C were intratracheally injected with one of the mycoplasma strains: Mycoplasma fermentans P 140 (wild strain, Mycoplasma fermentans PG 18 (type strain or Mycoplasma pneumoniae Eaton strain. Groups D, E, F were the negative, media, and sham controls. Fragments of trachea, lungs, kidney, heart, brain and spleen were cultured and used for the histopathological study. U frequency test was used to compare recovery of mycoplasmas from organs. Results Mycoplasmas were detected by culture and PCR. The three mycoplasma strains induced an interstitial pneumonia; they also migrated to several organs and persisted there for at least 50 days. Mycoplasma fermentans P 140 induced a more severe damage in lungs than Mycoplasma fermentans PG 18. Mycoplasma pneumoniae produced severe damage in lungs and renal damage. Conclusions Mycoplasma fermentans induced a respiratory tract infection and persisted in different organs for several weeks in hamsters. This finding may help to explain the ability of Mycoplasma fermentans to induce pneumonia and chronic infectious diseases in humans.

  14. Lung function and bronchial responsiveness after Mycoplasma pneumoniae infection in early childhood

    DEFF Research Database (Denmark)

    Boysen, Birgitte Kjær; Jensen, Jørgen S; Nielsen, Kim G

    2008-01-01

    follow-up examination including lung function testing (28 PCR-positive and 37 PCR-negative). In addition to the PCR-test for M. pneumoniae all respiratory tract specimens were additionally tested for other atypical bacteria and for viruses by PCR. Lung function was measured as specific airway resistance...... by whole-body plethysmography and bronchial hyperresponsiveness was assessed by cold, dry air hyperventilation. Neither baseline lung function nor bronchial response to cold dry air hyperventilation differed between M. pneumoniae-positive and -negative children: mean baseline lung function were 1.17 versus...... 1.21 (kPa sec), P = 0.45; and mean change in specific resistance was 13% versus 9%, P = 0.42. In conclusion, M. pneumoniae infection in early childhood was not associated with long-term effects on lung function and bronchial hyperresponsiveness 2 years after infection....

  15. 肺炎支原体感染的肺外表现%Extrapulmonary manifestations of Mycoplasma pneumoniae infection

    Institute of Scientific and Technical Information of China (English)

    李呈媚

    2012-01-01

    The pathogenesis of Mycoplasma pneumoniae (MP) is inclined to the two mechanisms of epithelial cell adhesion and immunological, which immune dysregulation may play an important role in the pathogenicity of extra-pulmonary damage of MP. The manifestations of its extra-pulmonary usually involves multiple systems, including nervous system, cardiovascular system, urinary system, skin tissue, immune system damage, muscle and joint damage. Extra-pulmonary damage of MP should be paid attention in clinical work.%肺炎支原体的致病机理目前倾向于上皮细胞吸附及免疫学两种机制.其中免疫调节异常在肺炎支原体损害致病中可能起重要作用.肺炎支原体的肺外表现通常涉及多个系统,包括神经系统、心血管系统、泌尿系统、皮肤组织、相关免疫系统损害、相关肌肉及关节损害.临床工作中应重视MP肺外损害表现.

  16. Periodicity in Attachment Organelle Revealed by Electron Cryotomography Suggests Conformational Changes in Gliding Mechanism of Mycoplasma pneumoniae

    Directory of Open Access Journals (Sweden)

    Akihiro Kawamoto

    2016-04-01

    Full Text Available Mycoplasma pneumoniae, a pathogenic bacterium, glides on host surfaces using a unique mechanism. It forms an attachment organelle at a cell pole as a protrusion comprised of knoblike surface structures and an internal core. Here, we analyzed the three-dimensional structure of the organelle in detail by electron cryotomography. On the surface, knoblike particles formed a two-dimensional array, albeit with limited regularity. Analyses using a nonbinding mutant and an antibody showed that the knoblike particles correspond to a naplike structure that has been observed by negative-staining electron microscopy and is likely to be formed as a complex of P1 adhesin, the key protein for binding and gliding. The paired thin and thick plates feature a rigid hexagonal lattice and striations with highly variable repeat distances, respectively. The combination of variable and invariant structures in the internal core and the P1 adhesin array on the surface suggest a model in which axial extension and compression of the thick plate along a rigid thin plate is coupled with attachment to and detachment from the substrate during gliding.

  17. Changes of Airway Reactivity after Mycoplasma Pneumoniae Infection in Children: A Study for Early Precautions against Pediatric Asthma.

    Science.gov (United States)

    Zhang, Han; Lv, Gaomei; Shang, Yunxiao; Liu, Liyun; Xiang, Yun; Feng, Jing; Wang, Zhijia

    2015-10-01

    The relationship between Mycoplasma pneumoniae (MP) infection and asthma has rarely been explored through examination of airway reactivity. The aim of this study was to determine airway reactivity changes after MP infection in children. First, 106 children were divided into four groups according to the existence of MP infection and/or asthma. Then children with only MP belonged to the MP group; children who had both MP infection and asthma belonged to the MP+A group; children with asthma but not MP infection belonged to the non-MP+A group; normal children were classified as normal control (NC) group. Each subject underwent a bronchial provocation test (BPT) after effectively controlling the symptoms. Airway hyperresponsiveness (AHR) parameters were compared among the groups. BPT positive rates were also calculated and compared. All AHR parameters decreased following MP infection, with a more significant decrease of small airway reactivity related indexes. The BPT-positive rate in the MP+A group was significantly higher than that in the MP group. Large airway reactivity showed no significant differences between the MP+A and non-MP+A groups, while the small airway reactivity augmented more significantly in the MP+A group. MP infection caused increased reactivity of both large and small airways in lungs, and BPT-positive identification in some patients.

  18. Molecular epidemiology of Mycoplasma hyopneumoniae from outbreaks of enzootic pneumonia in domestic pig and the role of wild boar.

    Science.gov (United States)

    Kuhnert, Peter; Overesch, Gudrun

    2014-11-07

    Mycoplasma hyopneumoniae is the major cause of enzootic pneumonia (EP) in domestic pigs, a disease with low mortality but high morbidity, having a great economic impact for producers. In Switzerland EP has been successfully eradicated, however, sporadic outbreaks are observed with no obvious source. Besides the possibility of recurrent outbreaks due to persisting M. hyopneumoniae strains within the pig population, there is suspicion that wild boars might introduce M. hyopneumoniae into swine herds. To elucidate possible links between domestic pig and wild boar, epidemiological investigations of recent EP outbreaks were initiated and lung samples of pig and wild boar were tested for the presence of specific genotypes by multilocus sequence typing (MLST). Despite generally different genotypes in wild boar, outbreak strains could be found in geographically linked wild boar lungs after, but so far not before the outbreak. Recurrent outbreaks in a farm were due to the same strain, indicating unsuccessful sanitation rather than reintroduction by wild boar. In another case outbreaks in six different farms were caused by the same strain never found in wild boar, confirming spread between farms due to hypothesized animal transport. Results indicate the presence of identical lineages of wild boar and domestic pig strains, and possible transmission of M. hyopneumoniae between wild boar and pig. However, the role of wild boar might be rather one as a recipient than a transmitter. More important than contact to wild boar for sporadic outbreaks in Switzerland is apparently persistence of M. hyopneumoniae within a farm as well as transmission between farms.

  19. Correlated response of peripheral blood cytokines with selection for reduced mycoplasma pneumonia of swine lesions in Landrace pigs.

    Science.gov (United States)

    Sato, Takumi; Okamura, Toshihiro; Kojima-Shibata, Chihiro; Kadowaki, Hiroshi; Suzuki, Eisaku; Uenishi, Hirohide; Suzuki, Keiichi

    2016-04-01

    Mycoplasma pneumonia of swine (MPS) is responsible for significant economic losses in the swine industry. We selected Landrace pigs for reduced MPS pulmonary lesions over five generations, and measured concentrations of the following cytokines: interleukin (IL)-10, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ to estimate their correlation with MPS lesions. Sheep red blood cells (SRBC) were injected twice intramuscularly at 70 and 95 kg body weight. Blood serum samples were collected after 1 week of secondary SRBC inoculation and cytokine concentrations were analyzed by ELISA. Genetic parameters and breeding values were estimated. The heritability estimates of IL-10, IL-13, IL-17, TNF-α and IFN-γ were 0.20 ± 0.06, 0.12 ± 0.06, 0.27 ± 0.07, 0.20 ± 0.10 and 0.05 ± 0.03, respectively. Genetic correlations of IL-17 and TNF-α with pulmonary MPS lesions were high (-0.86 ± 0.13 and 0.69 ± 0.29, respectively) and those of IFN-γ and IL-13 with MPS lesions were moderately negative (-0.45). Through selection, the breeding values of IL-17 and IFN-γ increased substantially and those of TNF-α decreased. These results suggest that innate and cellular immunity are more important for the suppression of pulmonary lesions in MPS than humoral-mediated immunity, such as antibody response.

  20. Effect of Qingre Jiedu Formula on Living Mycoplasma in Children with Refractory Mycoplasma Pneumonia%清热解毒方对难治性支原体肺炎患儿支原体活体的影响

    Institute of Scientific and Technical Information of China (English)

    杨会荣; 刘建华; 赵秀敏; 帅金凤; 牛波; 路素坤

    2014-01-01

    目的:探讨清热解毒方治疗对难治性支原体肺炎患儿支原体活体的影响。方法:选取本院难治性支原体肺炎住院患儿87例,治疗过程中因故不能坚持退出12例,最终入选75例,随机分为2组。对照组37例,采用阿奇霉素抗菌等治疗。治疗组38例,在阿奇霉素等治疗基础上加用清热解毒方(由生石膏、炙麻黄、桔梗、苦杏仁、连翘、蒲公英、鱼腥草、大青叶、白花蛇舌草等组成)。中药治疗1疗程前后分别采取2组患儿肺泡灌洗液,在超高倍镜下观察肺炎支原体活体变化。结果:治疗后治疗组与对照组患儿肺泡灌洗液支原体活体明显减少,二者比较差异有显著性(P <0.05)。结论:抑制肺炎支原体活体可能是清热解毒方治疗难治性支原体肺炎的作用机制之一。%Objective:to explore the effect of Qingre Jiedu formula on living mycoplasma in children with refractory mycoplasma pneumonia. Methods:87 children with refractory mycoplasma pneumonia in the hospital,but finally 75 patients were chosen in the study since 12 patients disengaged for some reasons,among whom 37 patients was control group treated with Azithromycin while the other 38 patients was treatment group treated with Qingre Jiedu formula in addition to Azithromycin. The formula consists of Sheng Shi-gao (gypsum),Jiu Mahuang (Ephedra),Jiegeng (root of balloonflower),Ku Xingren (ansu apricot seed),Lianqiao (weeping for-sythia fruit),Pugongying (dandelion),Yuxingcao (heartleaf houttuynia herb),Da Qingye (folium isatidis)and Bai Hua She She Cao (oldenlandia),and so on. After one course of treatment with Chinese medicine,patients of both group were given alveolar lavage fluid. The living mycoplasma changes were observed under the ultramicroscope. Results:The living mycoplasma number in treatment group was reduced significantly compared to control group,and the difference was significant (P <0.05 ). Conclusion

  1. Role of Climate factors on Mycoplasma Pneumoniae Pneumonia in Children%气候因素对于儿童肺炎支原体肺炎的作用研究

    Institute of Scientific and Technical Information of China (English)

    倪慧萍; 季伟; 陈正荣

    2014-01-01

    [ABSTRACT]Objective:To study the role of climate factors on Mycoplasma pneumoniae (MP) pneumonia in Children in Suzhou, China. Methods:Due to acute pneumonia and 10,596 cases of children hospitalized for multi-pathogen detection period 2006~2011, in order to clear Mycoplasma pneumoniae infection.Also collected from 2006 to 2011 in Suzhou relevant meteorological data.Results:Mycoplasma pneumoniae pneumonia accounted for 29.28%of all children with pneumonia (3102/10596);MP incidence of pneumonia all year round and seasonal, occur in the summer, the detection rate of 36.13%.Positive correlation between the presence of moderate pneumonia MP detection rate and monthly average temperature (rs=0.501, P<0.0001).Another MP pneumonia detection rate and monthly total rainfall was also positively correlated, but the correlation is weak (rs=0.276, P=0.019).Stepwise regression analysis showed that differences in the incidence of mycoplasma pneumonia by 49%due to the temperature (β=0.49, t=4.697, P<0.0001), and the average temperature rise 1℃, MP pneumonia increased incidence of 2.2%.The detection rate of children with MP pneumonia and can be predicted by the model.Conclusion:MP was one of the most common pathogen in Suzhou according 6-year pathogen surveillance.MP penumonia has its seasonality.Mean temperature was the main factor affecting the incidence of MP pneumonia.%目的:探讨肺炎患儿肺炎与气候因素之间的相关性。方法:2006~2011年期间由于急性肺炎而住院的10596例儿童进行多病原检测,以明确肺炎支原体感染。同时收集2006~2011年苏州地区有关气象资料。结果:肺炎支原体肺炎占所有肺炎患儿的29.28%(3102/10596);MP肺炎全年均可发病,并具有季节性,好发于夏季,检出率高达36.13%。肺炎患儿MP检出率与月平均气温之间存在中度正相关(rs=0.501, P<0.0001)。另外肺炎患儿MP检出率与月总雨量也呈正相关,

  2. 小儿肺炎支原体肺炎临床治疗护理体会%Experience of clinical nursing on children with mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    曹春梅

    2014-01-01

    Objective:To investigate the effectiveness nursing care on children with mycoplasma pneumonia.Methods:40 children with mycoplasma pneumoniae pneumonia were selected from January 2013 to February 2014.Analyze their nursing condition retrospectively.They were randomly divided into the treatment group and the control group with 20 cases in each.The control group were given general nursing.The treatment group were given holistic nursing based on general nursing,including pediatric drugs,psychological guidance,respiratory tract nursing,discharge guidance of parents,children and home health guidance,etc. Results:The control group received general nursing,and the effective rate was 70%.The treatment group received holistic nursing care,and the effective rate was 95%.The difference was statistically significant between the two groups(P<0.05).Conclusion:The treatment effect of holistic nursing care in patients with mycoplasma pneumonia was significantly higher than that of general nursing.This nursing mode of mycoplasma pneumonia worthy of clinical promotion.%目的:探讨对小儿肺炎支原体感染的有效性护理。方法:2013年1月-2014年2月收治肺炎支原体肺炎患儿40名,对其护理情况进行回顾性分析。随机分成治疗组和对照组各20例。对照组进行一般的基础护理,治疗组在一般护理基础上,进行整体护理,包括小儿的药物指导、心理、呼吸道护理、出院指导、患儿家长的指导和院外的健康讲座等。结果:对照组经一般护理,治疗有效率70%,治疗组经整体护理,有效率95%,经统计学比较差异有统计学意义(P<0.05)。结论:小儿肺炎支原体肺炎临床治疗护理中,经整体护理的患者其治疗效果明显高于一般护理,值得在临床推广。

  3. Exploration on Nursing of Mycoplasma Pneumonia in Children Lobar Pneumonia%儿童肺炎支原体性大叶性肺炎的护理探索

    Institute of Scientific and Technical Information of China (English)

    朱莉

    2016-01-01

    文章主要探讨儿童肺炎支原体感染性大叶性肺炎的临床表现及护理对策。通过回顾性分析儿科住院且确诊的179例肺炎支原体感染性大叶性肺炎患儿的临床表现,提出相应的护理对策,即认真、细致地观察病情,给予全面、合理、有效的护理,并配合积极的治疗,对疾病的治愈起到关键的作用。%This article investigates the clinical manifestations and nursing countermeasures of lobar pneumonia mycoplasma pneumonia infection in children. This paper makes a retrospective analysis of hospitalized and diagnosed 179 cases of mycoplasma pneumonia infection of lobar pneumonia in children clinical manifestations. And we also give the corresponding nursing countermeasures including serious and careful observation of the disease, giving a comprehensive, reasonable and effective care with the active treatment, which plays a key role in the cure of the disease.

  4. Detection of Mycoplasma pneumoniae in cerebrospinal fluid of children: Quantification of CSF-IgG antibody

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    Noorbakhsh S

    2010-08-01

    Full Text Available "nBackground: M. pneumoniae infection in children is usual and diagnosis of its neurologic complications for rapid treatment is very important. To compare the CSF- M. pneumoniae antibody level between febrile children with acute neurologic signs (Menigoencephalitis, Guillan Barre Syndrome (GBS, Transverse myelitis, Ataxia and so on with unaffected ones. "n"nMethods: A cross sectional/ case control study in pediatric wards of Rasoul-e-Akram & Mofid hospitals (2007-2009 was done. The amount of Specific M. pneumoniae IgG (ELISA antibody level determined in CSF of 55 cases and in 10 controls. Chi square values (CI 95%, p< 0.05 calculated for all categorical variables. Sensitivity; specificity; Positive Predictive Value (PPV; Negative Predictive Value (NPV of CSF antibody level determined by using the Area under the ROC Curve. "n"nResults: Cases (n= 55 aged between five month to 13 years with mean age of 3.84±3.43 years. Area Under Curve (AUC in ROC was 0.876 (%95 CI, 0.78- 0.96; p< 0.0001. Cut off level for antibody was 0.0025 with 73% sensitivity; 90% specificity; 100% PPV; 28.8% NPV. CSF antibody level had significant difference between cases and controls [0.08± 0.26 Versus 0.001± 0.001; p: 0.02]; It had poor agreement between cases and controls (Kappa= 0.27. Lowest amount seen in cases with aseptic meningitis; highest amount observed in cases with GBS and cases with focal neurologic signs. "n"nConclusion: The presence of very low amount (0.0025 of M. pneumoniae antibody in CSF of febrile children with acute neurologic signs had 70% sensitivity and 90% specificity; 100% PPV; but had low (28.8% NPV. M. pneumoniae would be a rare cause in cases with aseptic meningitis. Finding the M. pneumoniae-DNAs in CSF are not so frequent (2% but in high suspicious cases adding this test to determining the CSF antibody level might be helpful.

  5. Comparison of three different novel Chlamydophila pneumonia recombinant antigens for serodiagnosis%肺炎嗜衣原体三种不同抗原的临床诊断价值评价

    Institute of Scientific and Technical Information of China (English)

    陈虹亮; 胡旃; 周洲; 曾焱华; 代国知; 林应标; 吴移谋

    2010-01-01

    Objective To evaluate three different Chlamydophila pneumoniae recombinant antigens for use in Chlamydophila pneumoniae serodiagnosis. Methods The recombinant plasmids pGEX6p-2/ Cpn0146,Cpn0147 and Cpn0308 were constructed and expressed as GST fusion proteins. The immunogenicity and the immunocompetence of these recombinant protein were analyzed by Western-blot and indirect ELISA. A total of 183 sera samples of patients with respiratory tract infection and 32 sera samples of patients with Chlamydia trachomatis infection were detected with indirect ELISA coated microwell plates with the purified recombinant proteins comparing with SeroCP-TM IgG ELISA kits. The positive recognition rate, sensitivity and specificity of each method were analyzed. Results GST-Cpn0146, Cpn0147 and Cpn0308 were obtained after expression and purification. The titers of the specific IgG antibodies against Cpn0146, Cpn0147 and Cpn0308 were higher than 1:6 400, 1:128 00 and 1:128 00, respectively. When the indirect ELISA was developed to detect the IgG antibody against Chlamydophila pneumoniae in 183 samples, the concordance rate between the indirect ELISA test and SeroCP-TM IgG ELISA kits were 92. 3% (Cpn0146) , 94.5% (Cpn0147) and 96.7% (Cpn0308), respectively. The recombinant Cpn0146, Cpn0147 and Cpn0308 were recognized by 71 (38.8% positive recognition rate), 75 (40.9%), and 82 (44.8%) samples, respectively. The recombinant antigen-based detection assays displayed > 97% of detection specificity and>87%of sensitivity.Condusion GST-Cpn0308 shows a better sensitivity and specificity,which suggests it could be used for developing serodiagnosis kits of Chlamydophila pneumoniae infection.%目的 研究肺炎嗜衣原体3种重组抗原Cpn0146、Cpn0147及Cpn0308应用于肺炎嗜衣原体感染血清学诊断中的价值.方法 构建pGEX6p-2/Cpn0146、Cpn0147、Cpn0308重组质粒,并诱导表达重组蛋白,采用间接ELISA法和免疫印迹法(Western-blot)分析其免疫原性及其

  6. Regimen analysis of children infected by macrolide-resistant mycoplasma pneumoniae%耐药肺炎支原体感染患儿的用药分析

    Institute of Scientific and Technical Information of China (English)

    王穗琼; 王豫; 邓文喻; 张琳

    2013-01-01

    Objective To explore the regimen of children infected by macrolide - resistant mycoplasma pneumoniae. Methods The regimens were analyzed of children whose throat swab mycoplasma pneumoniae cultures were positive, from November 2010 to May 2012 in our hospital. And the courses of medications were returned visits. Results There were 116 children whose throat swab mycoplasma pneumoniae cultures were positive. All of them took macrolide antibacterial agents. In addition to three children combined (3-lactam antibacterials, there were 49 children in the drug - resistant group of 52 children infected by macrolide - resistant mycoplasma pneumoniae. There were 64 children in the drug - susceptive group. No significant differences were found in sex, age, usage rates of macrolide antibacterial agents, antipyretic drugs, antihistamines and phlegm drugs, P > 0. 05. Compared to drug - susceptive group, the courses of macrolide antibacterial agents were shorter, and antipyretic drugs, antihistamine drugs and apophlegmatisants were longer in the drug -resistant group, P < 0. 05. Conclusions The children infected by macrolide - resistant mycoplasma pneumoniae treated by antipyretic drugs, antihistamine drugs and apophlegmatisants. Compared to the children infected by macrolide - susceptive mycoplasma pneumoniae, the children infected by macrolide - resistant had shorter course of macrolide antibacterial agents, and longer courses of antipyretic drugs, antihistamine drugs and apophlegmatisants.%目的 探讨耐大环内酯类抗菌药的肺炎支原体感染患儿的用药方案.方法 分析2010年11月至2012年5月广东省妇幼保健院收治的咽拭子肺炎支原体培养阳性患儿的用药方案,并回访用药疗程.结果 MP培养阳性患儿116例均使用了大环内酯类抗菌药抗感染治疗,其中耐药52例,除去3例联合了β内酰胺类抗菌药,耐药组为49例;敏感组为64例,没有联合β内酰胺类抗菌药病例.两组

  7. Comparative "-omics" in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors.

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    Maria Lluch-Senar

    Full Text Available The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2, differing in the sequence of the P1 adhesin gene. Here, we have sequenced the genomes of 23 clinical isolates of M. pneumoniae. Studying SNPs, non-synonymous mutations, indels and genome rearrangements of these 23 strains and 4 previously sequenced ones, has revealed new subclasses in the two main groups, some of them being associated with the country of isolation. Integrative analysis of in vitro gene essentiality and mutation rates enabled the identification of several putative virulence factors and antigenic proteins; revealing recombination machinery, glycerol metabolism and peroxide production as possible factors in the genetics and physiology of these pathogenic strains. Additionally, the transcriptomes and proteomes of two representative strains, one from each of the two main groups, have been characterized to evaluate the impact of mutations on RNA and proteins levels. This study has revealed that type 2 strains show higher expression levels of CARDS toxin, a protein recently shown to be one of the major factors of inflammation. Thus, we propose that type 2 strains could be more toxigenic than type 1 strains of M. pneumoniae.

  8. Clinical Studies 46 cases of Mycoplasma Pneumoniae Infection in Children%小儿肺炎支原体感染46例临床研究

    Institute of Scientific and Technical Information of China (English)

    孔馥

    2014-01-01

    Objective Mycoplasma pneumoniae infection in children with clinical diagnosis and treatment are discussed.Methods Mycoplasma pneumoniae in children in our hospital 46 cases of infection in children as an object,the implementation of children with clinical diagnosis and treatment,the prognosis observed in children.Results The incidence of Mycoplasma pneumoniae in children with no correlation between age and season,the majority of patients with pulmonary symptoms,admission diagnosis display 18 cases diagnosed in children,discharge diagnosis was confirmed,the admission diagnosis was 39.1%.Show all patients were treated with antibiotics to cure.Conclusion For children with suspected Mycoplasma pneumoniae infection in children,the need for timely diagnosis and treatment,the treatment,and clinical efficacy of azithromycin kitasamycin significant,it is worth popularity.%目的:对小儿肺炎支原体感染的临床诊治进行探讨。方法选取我院收治的46例小儿肺炎支原体感染患儿为对象,对患儿实施临床诊断和治疗,观察患儿的预后。结果小儿肺炎支原体的发病率与年龄和季节无相关性,多数患者合并肺外症状,入院诊断显示18例患儿确诊,出院诊断均确诊,出入院诊断符合率为39.1%。抗生素治疗显示患儿均全部治愈。结论对于疑似小儿肺炎支原体感染的患儿,临床上需要及时进行诊断和治疗,在治疗上,红霉素和阿奇霉素的临床疗效显著,值得普及。

  9. 小儿肺炎支原体肺炎的临床特点及护理%Clinical Characteristics and Nursing Care of Children with Mycoplasma Pneumonia

    Institute of Scientific and Technical Information of China (English)

    阿孜古力•艾合麦提; 王慧颖; 王洁

    2016-01-01

    Objective:To investigate the clinical features and nursing care of Mycoplasma pneumoniae pneumonia in children. Methods:Extraction from 100 cases of infantile pneumonia mycoplasma pneumonia children were analysis, were randomly divided into two groups, patients in the control group received the routine nursing intervention, patients in the observation group in the implementation of comprehensive nursing intervention, clinical effects were compared between the two groups. Results:through the clinical effects were compared between the two groups, observation group was significantly better than the control group, significant difference (P>0.05), with statistical significance. Conclusion: for children with Mycoplasma pneumoniae pneumonia in children with implementation of comprehensive nursing intervention can improve the symptoms of hypoxia in children, effectively reduce the complications and promote Children with rehabilitation, suitable for clinical promotion and application.%目的:探讨小儿肺炎支原体肺炎的临床特点及护理。方法:抽取收治的100例小儿肺炎支原体肺炎患儿进行分析研究,进行随机分组,对照组患儿实施常规护理干预,观察组患儿实施综合性护理干预,对比两组的临床效果。结果:通过对比两组的临床效果,观察组明显优于对照组,差异显著,(P>0.05),具有统计学意义。结论:针对小儿肺炎支原体肺炎患儿实施综合性护理干预,能够改善患儿缺氧症状,有效降低并发症的发生,促进患儿康复,适合临床的推广与运用。

  10. Analysis on clinical diagnosis and treatment of Mycoplasma pneumoniae pneumonia in children%小儿肺炎支原体肺炎的临床诊治分析

    Institute of Scientific and Technical Information of China (English)

    闫文芳

    2012-01-01

    目的:探讨小儿肺炎支原体肺炎的临床特点,提高小儿肺炎诊治水平.方法:回顾性分析该院在2011年5月~2012年5月收治并经过临床确诊的120例患者的临床资料.结果:120例患儿经过3~5天临床治疗后有99例患儿体温明显下降,咳嗽明显减轻.治疗7~9天后有18例患儿体温平稳,咳嗽明显减轻,其中有3例患儿合并军团菌感染高热持续11天后体温才恢复正常,临床对于合并其他的细菌感染18例患者给予头孢类或美洛西林治疗,对该组患儿临床经过12天治疗后经过复查X线胸片好转后出院进行随访,经1个月随访,所有患儿全部治愈.结论:小儿肺炎支原体肺炎是临床常见的疾病,临床表现为除呼吸道疾病外,常合并肺外多系统的损害,咽拭子支原体DNA检测阳性可以作为临床早期诊断的重要手段,对于小儿肺炎支原体肺炎临床采用阿奇霉素和红霉素治疗效果好.%Objective; To explore the clinical characteristics of Mycoplasma pneumoniae pneumonia in children, and improve the diagnostic level and curative level. Methods: The clinical data of 120 children who were diagnosed as Mycoplasma pneumoniae pneumonia definitely and treated in the hospital from May 2011 to May 2012 were analyzed retrospectively. Results; At 3 - 5 days after clinical treatment, body temperature of 99 children decreased significantly, cough was relieved obviously. At 7 -9 days after treatment, the body temperature of 18 children maintained stable, cough was relieved obviously, and the body temperature of 3 children with Legionella,infection combined with hyperpyrexia returned to normal after 11 days. Eighteen children with other bacterial infection were treated with cephalosporin and mezlocillin for 12 days, then they left the hospital after improvement of X - ray chest radiography reexamination, the children were followed up for more than one month, all the children were cured. Conclusion: Pediatric Mycoplasma

  11. Zoonotic potential of Chlamydophila.

    Science.gov (United States)

    Rodolakis, Annie; Yousef Mohamad, Khalil

    2010-01-27

    The purpose of this article is to present the diseases induced in humans and animals by the different species of Chlamydophila, after providing an overview on the history of these infectious agents and their taxonomy. The route of transmission and the available methods for prevention and control in the different animal species are reviewed.

  12. Chlamydophila pneumoniae induces expression of Toll-like Receptor 4 and release of TNF-α and MIP-2 via an NF-κB pathway in rat type II pneumocytes

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    Maass Matthias

    2005-06-01

    Full Text Available Abstract Background The role of alveolar type II cells in the regulation of innate and adaptive immunity is unclear. Toll-like receptors (TLRs have been implicated in host defense. The purpose of the present study was to investigate whether Chlamydophila pneumoniae (I alters the expression of TLR2 and/orTLR4 in type II cells in a (II Rho-GTPase- and (III NF-κB-dependent pathway, subsequently (IV leading to the production of (IV pro-inflammatory TNF-α and MIP-2. Methods Isolated rat type II pneumocytes were incubated with C. pneumoniae after pre-treatment with calcium chelator BAPTA-AM, inhibitors of NF-κB (parthenolide, SN50 or with a specific inhibitor of the Rho-GTPase (mevastatin. TLR2 and TLR4 mRNA expressions were analyzed by PCR. Activation of TLR4, Rac1, RhoA protein and NF-κB was determined by Western blotting and confocal laser scan microscopy (CLSM and TNF-α and MIP-2 release by ELISA. Results Type II cells constitutively expressed TLR4 and TLR2 mRNA. A prominent induction of TLR4 but not TLR2 mRNA was detected after 2 hours of incubation with C. pneumoniae. The TLR4 protein expression reached a peak at 30 min, began to decrease within 1–2 hours and peaked again at 3 hours. Incubation of cells with heat-inactivated bacteria (56°C for 30 min significantly reduced the TLR4 expression. Treated bacteria with polymyxin B (2 μg/ml did not alter TLR4 expression. C. pneumoniae-induced NF-κB activity was blocked by TLR4 blocking antibodies. TLR4 mRNA and protein expression were inhibited in the presence of BAPTA-AM, SN50 or parthenolide. TNF-α and MIP-2 release was increased in type II cells in response to C. pneumoniae, whereas BAPTA-AM, SN50 or parthenolide decreased the C. pneumoniae-induced TNF-α and MIP-2 release. Mevastatin inhibited C. pneumoniae-mediated Rac1, RhoA and TLR4 expression. Conclusion The TLR4 protein expression in rat type II cells is likely to be mediated by a heat-sensitive C. pneumoniae protein that induces a

  13. Hydrogen sulfide is a novel potential virulence factor of Mycoplasma pneumoniae: characterization of the unusual cysteine desulfurase/desulfhydrase HapE.

    Science.gov (United States)

    Großhennig, Stephanie; Ischebeck, Till; Gibhardt, Johannes; Busse, Julia; Feussner, Ivo; Stülke, Jörg

    2016-04-01

    Mycoplasma pneumoniae is a human pathogen causing atypical pneumonia with a minimalized and highly streamlined genome. So far, hydrogen peroxide production, cytadherence, and the ADP-ribosylating CARDS toxin have been identified as pathogenicity determinants. We have studied haemolysis caused by M. pneumoniae, and discovered that hydrogen peroxide is responsible for the oxidation of heme, but not for lysis of erythrocytes. This feature could be attributed to hydrogen sulfide, a compound that has previously not been identified as virulence factor in lung pathogens. Indeed, we observed hydrogen sulfide production by M. pneumoniae. The search for a hydrogen sulfide-producing enzyme identified HapE, a protein with similarity to cysteine desulfurases. In contrast to typical cysteine desulfurases, HapE is a bifunctional enzyme: it has both the cysteine desulfurase activity to produce alanine and the cysteine desulfhydrase activity to produce pyruvate and hydrogen sulfide. Experiments with purified HapE showed that the enzymatic activity of the protein is responsible for haemolysis, demonstrating that HapE is a novel potential virulence factor of M. pneumoniae.

  14. Critical role of macrophages and their activation via MyD88-NFκB signaling in lung innate immunity to Mycoplasma pneumoniae.

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    Jen-Feng Lai

    Full Text Available Mycoplasma pneumoniae (Mp, a common cause of pneumonia, is associated with asthma; however, the mechanisms underlying this association remain unclear. We investigated the cellular immune response to Mp in mice. Intranasal inoculation with Mp elicited infiltration of the lungs with neutrophils, monocytes and macrophages. Systemic depletion of macrophages, but not neutrophils, resulted in impaired clearance of Mp from the lungs. Accumulation and activation of macrophages were decreased in the lungs of MyD88(-/- mice and clearance of Mp was impaired, indicating that MyD88 is a key signaling protein in the anti-Mp response. MyD88-dependent signaling was also required for the Mp-induced activation of NFκB, which was essential for macrophages to eliminate the microbe in vitro. Thus, MyD88-NFκB signaling in macrophages is essential for clearance of Mp from the lungs.

  15. Participação do Mycoplasma pneumoniae na etiologia de infecções respiratórias agudas em Ribeirão Preto, São Paulo, Brasil

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    Alcyone A. Machado

    1991-03-01

    Full Text Available Para avaliar a participação etiológica do Mycoplasma pneumoniae em infecções respiratórias agudas, o isolamento dessa bactéria foi tentado em secreções do aparelho respiratório de 64 pacientes (média 24 anos com quadro respiratório aguda Foi realizada, também, a pesquisa de anticorpos específicos anti-M. pneumoniae através da reação de fixação do complemento (FC e da reação de contra-imunoeletroforese (CIE. O M. pneumoniae não foi isolado. O diagnóstico presuntivo de infecção pelo M. pneumoniae foi feito pela FC em 3,1% (2/64 e pela CIE em 1,6% (1/64 dos pacientes. Paralelamente, em 200 indivíduos sadios, os mesmos testes sorológicos foram realizados, sendo o índice de positividade de 4% (8/200 pela CIE e de 1% (2/200 pela FC. Apesar das aiscrepâncias observadas entre os dois métodos sorológicos, a FC parece ser indicada para diagnóstico da infecção, sendo a CIE recomendada nas avaliações soroepidemiológicas. Com base nos dados do nosso estudo, a prevalência das infecções respiratórias pelo M. pneumoniae parece ser baixa em nosso meio.Mycoplasma pneumoniae isolation was attempted in respiratory fluids from 64 patients with respiratory infection Complement fixation test (CF and counter- immunoelectrophoresis (CIE were used for Mycoplasma antibody detection using the patient sera. Mycoplasma pneumoniae was not isolated. Serologic diagnosis were positives in 3,1% (2/64 by CF test and 1,6% (1/64 by CIE. Serologic tests done in 200 health controls showed 4% (8/200positives by CIE and 1 % (2/200 by CF. The results showed differences in sensitivity among the serologic tests. CF seems to be more indicated for Mycoplasma infection diagnosis while, CIE could be used for Mycoplasmas serosurveys. The prevalence of Mycoplasma pneumoniae infections was low (3,1% in the 64 patients during our study period.

  16. 呼吸道感染患者外周血中肺炎嗜衣原体的检测与分析%Detection and analysis for Chlamydophila pneumoniae in peripheral blood in patients with respiratory tract infection

    Institute of Scientific and Technical Information of China (English)

    吴华; 吴移谋; 黄志坚; 詹济舟; 刘良专

    2011-01-01

    In order to investigate the infection rate of Chlamydophila pneumoniae, blood samples were collected from 173 respiratory tract infection patients and 52 healthy volunteers. DNA was extracted from PBMC and sera were isolated from the blood samples. The MOMP gene of C. pneumoniae were detected by PCR from the DNA samples and the C. pneumoniae specific IgG were measured by ELISA. PCR products were detected by 1.5% agarose gel electrophoresis and sequenced by Sangon Company. Combined with the clinic data, the difference of C. pneumoniae infected rate in different age group were analysed. The results of PBMC extraction were as follow: there're 173 people in the respiratory tract infection group, and the PCR (MOMP) positive rate was 32.40%(56/173); there're 52 people in the healthy group, and the PCR(MOMP) positive rate was 9.62% (5/52). The MOMP PCR product with Cpn AR-39 of the MOMP gene was 99% consistent. According to the positive rate of each group, the C. pneumoniae infection rate in the aged group was the highest. It's implied that Cpn is one of the most important respiratory tract pathogen and proved to be the main pathogen for C. pneumoniae. The older people are in highrisk group for C. pneumoniae infection.%目的 检测并分析呼吸道疾病患者的肺炎嗜衣原体(Cpn)感染率.方法 收集173例呼吸道感染患者和52例健康体检者外周血标本,分离外周血单核细胞(PBMC)血清.提取纯化PBMC的DNA,以该DNA为模板,用特异性引物体外扩增Cpn MOMP基因.扩增产物经电泳及测序鉴定,确认为Cpn特异性的目的 基因.用ELISA法检测待测血清中的Cpn-IgG抗体.通过PCR与ELISA的检测结果,结合临床资料进行流行病学综合分析Cpn感染率.结果 以PBMC基因组DNA为模板,呼吸道感染组患者标本中Cpn MOMP阳性率为32.40%(56/173),健康对照组中阳性率为9.62 oA(5/52);经测序比对,所有MOMP阳性扩增产物与Cpn AR-39的MOMP基因同源性在99%以上.ELISA检测血

  17. Clinical analysis of Mycoplasma pneumoniae infection in 30 cases of adult%30例成人肺炎支原体感染的临床分析

    Institute of Scientific and Technical Information of China (English)

    王娟; 杨兴仁; 李东升

    2014-01-01

    Objective:To investigate Mycoplasma pneumoniae infection in adult clinical features.Methods:The clinical data of 30 cases of Mycoplasma pneumoniae infection in adult patients were analyzed retrospectively.Results:The Results of this group of patients with multiple cough /or fever as the first symptom,mostly manifested as persistent paroxysmal cough,some patients myalgia,arthralgia,nausea,vomiting,diarrhea and other extrapulmonary symptoms. Lung X-ray manifestation lack of specificity,serum mycoplasma antibody (MP ~IgM)to detect meaningful.30 cases after treatment with azithromycin Se-quential cured 24 cases,effective in 6 cases,total effective rate was 100%,without sequelae.Conclusions:Master Mycoplasma pneumoniae infection in adult clinical features are critical for proper diagnosis and treatment.%目的:探讨成人肺炎支原体感染的临床特点。方法:对30例成人肺炎支原体感染患者的临床资料进行回顾性分析。结果:本组患者多以咳嗽伴/或发热为首发症状,大多表现为顽固的阵发性咳嗽,部分患者出现肌痛,关节痛,恶心、呕吐、腹泻等肺外症状。肺部X线片表现多样缺乏特异性,血中支原体抗体(MP~IgM)检测有意义。30例经阿奇霉素贯序治疗后,治愈24例,显效6例,总有效率为100%,无后遗症。结论:掌握成人肺炎支原体感染的临床特点是正确诊治的关键。

  18. 小儿肺炎支原体肺炎诊断治疗效果观察%Observation on diagnosis and therapeutic effect of mycoplasma pneumoniae pneumonia in children

    Institute of Scientific and Technical Information of China (English)

    王冰

    2016-01-01

    Objective:To explore the diagnostic methods and therapeutic effect of mycoplasma pneumoniae pneumonia in children.Methods:110 cases of children with mycoplasma pneumoniae pneumonia were divided into the observation group and the control group randomly,with 55 cases in each group.The control group was treated with erythromycin,and the observation group was treated with azithromycin.The clinical effect of the two groups was compared.Results:The total effective rate of the observation group was significantly higher than that of the control group(P<0.05).The disappearing time of pulmonary rales and relief time of cough in the observation group were significantly shorter than those in the control group (P<0.05).Conclusion:Azithromycin in the treatment of mycoplasma pneumoniae pneumonia had significant curative effect,which could alleviate the clinical symptoms of children effectively and had less adverse reactions.%目的:探讨小儿肺炎支原体肺炎的诊断方法与治疗效果。方法:收治肺炎支原体肺炎患儿110例,随机分为观察组和对照组,每组55例。对照组采用红霉素治疗,观察组采用阿奇霉素治疗,比较两组临床疗效。结果:观察组治疗总有效率明显高于对照组(P<0.05)。观察组肺部啰音消失时间、咳嗽缓解时间均明显短于对照组(P<0.05)。结论:阿奇霉素治疗肺炎支原体肺炎疗效显著,能够有效缓解患儿临床症状,且不良反应少。

  19. Analysis of clinical diagnosis and treatment of mycoplasma pneumoniae pneumonia in children%小儿肺炎支原体肺炎的临床诊治分析

    Institute of Scientific and Technical Information of China (English)

    齐俊民

    2015-01-01

    Objective:To analyze the clinical characteristics of mycoplasma pneumoniae pneumonia in children,thereby enhancing children mycoplasma pneumonia clinical diagnosis and treatment.Methods:120 patients with mycoplasma pneumoniae pneumonia in children were selected from January to December 2013.The clinical data were retrospectively analyzed,and they were divided into the treatment group and the control group.Results:The effective rate of treatment group was significantly higher than the control group,and there was statistically significant(P<0.05);the treatment group in the mean time coughing subsided,the average time of fever and average hospitalization time were far less than that in control group(P<0.05).Conclusion:The treatment group and the control group for comparison,the efficacy of the treatment group is significant;the treatment time is short;it can save medical costs for patients;it is worth promoting.%目的:对小儿肺炎支原体肺炎的临床表现特点进行分析,从而提高小儿肺炎支原体肺炎的临床诊治水平。方法:2013年1-12月收治小儿肺炎支原体肺炎患者120例,对临床治疗资料进行回顾性分析,将对其,分为治疗组和对照组。结果:治疗组有效治疗率显著高于对照组,两组比较差异有统计学意义(P<0.05);治疗组患儿的咳嗽消退平均时间、退热平均时间以及住院平均时间均要远远短于对照组(P<0.05)。结论:治疗组和对照组相对比,治疗组疗效显著,而且治疗时间短,为患者节省医疗费用,值得临床推广应用。

  20. Expression and significance of TNF-α, IL-6, and IL-10 in the serum in patients with mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    Dong-Hai Zhang

    2016-01-01

    Objective:To explore the expression and clinical significance of TNF-α, IL-6, and IL-10 in the serum in patients with mycoplasma pneumonia (MPP).Methods:A total of 120 patients with MPP who were admitted in our hospital from August, 2014 to June, 2015 were included in the study and served as the observation group, while 60 healthy children who came for physical examinations were served as the control group. The serum levels of IL-6, IL-10, and TNF-α in the observation group at the acute phase and recovery phase were detected and compared with the control group. The serum levels of IL-6, IL-10, and TNF-α in mild and severe MPP patients were compared. The levels of IL-6, IL-10, and TNF-α in the serum and pleural fluid in MPP patients with or with no fibrosis change were compared and analyzed. Results:When compared with the control group, the serum TNF-α and IL-6 levels in the observation group at the acute phase and recovery phase were significantly elevated, while IL-10 level was significantly reduced. The serum TNF-α and IL-6 levels in MPP patients at the acute phase were significantly lower than those at the recovery phase, while IL-10 level was significantly higher than that at the recovery phase. The serum TNF-α and IL-6 levels in the severe group were significantly higher than those in the mild group, while IL-10 level was significantly lower than that in the mild group. TNF-α level in the pleural fluid in MPP patients with fibrosis change was significantly higher than that in MPP patients with no fibrosis change, while the comparison of IL-6 and IL-10 was not statistically significant. TNF-α level in MPP patients with fibrosis change in the pleural fluid was significantly higher than that in the serum, while the comparison of IL-6 and IL-10 was not statistically significant. IL-6, IL-10, and TNF-α levels in MPP patients with no fibrosis change in the pleural fluid were not statistically different from those in the serum.Conclusions:TNF-α, IL-6, and

  1. Identification, sequencing and molecular analysis of Chp4, a novel chlamydiaphage of Chlamydophila abortus belonging to the family Microviridae.

    Science.gov (United States)

    Sait, Michelle; Livingstone, Morag; Graham, Rebecca; Inglis, Neil F; Wheelhouse, Nick; Longbottom, David

    2011-07-01

    Members of the family Microviridae have been identified in a number of chlamydial species infecting humans (phage CPAR39 in Chlamydophila pneumoniae), other mammals (φCPG1 in Chlamydophila caviae, Chp2 in Chlamydophila abortus and Chp3 in Chlamydophila pecorum) and birds (Chp1 in Chlamydophila psittaci). This study describes the identification and genome sequencing of Chp4, an icosahedral, 4530 bp, ssDNA phage in C. abortus. Chp4 is predicted to contain eight ORFs, six of which could be assigned putative functions based on sequence similarity to characterized bacteriophage. Gene order and content were highly conserved amongst chlamydiaphage, with the highest sequence variability occurring in the IN5 and INS variable regions of the VP1 major coat protein, which has been associated with host cell recognition and binding. Phylogenetic analysis of VP1 indicated that Chp4 is a member of the Chlamydiamicrovirus, and is most closely related to phage φCPG1 and CPAR39.

  2. 21 CFR 866.3375 - Mycoplasma spp. serological reagents.

    Science.gov (United States)

    2010-04-01

    .... The identification aids in the diagnosis of disease caused by bacteria belonging to the genus Mycoplasma and provides epidemiological information on diseases caused by these microorganisms. Mycoplasma... infection to mild or severe upper respiratory disease, ear infection, and bronchial pneumonia....

  3. Mycoplasma pneumoniae in elderly patients with stroke. a case-control study on the seroprevalence of M. pneumoniae in elderly patients with acute cerebrovascular disease - the M-PEPS Study.

    Science.gov (United States)

    Ngeh, Joseph; Gupta, Sandeep; Goodbourn, Colin; McElligott, Geraldine

    2004-01-01

    Previous studies have suggested certain infections as potential risk factors for stroke. Chlamydia pneumoniae, an atypical respiratory pathogen, has been linked to atherosclerotic vascular diseases. Mycoplasma pneumoniae, another atypical respiratory micro-organism, can rarely cause stroke. We investigated whether serological markers of M. pneumoniae infection were associated with acute stroke or transient ischaemic attack (TIA) in elderly patients. This case-control study was nested within the C-PEPS study - a case-control study on the seroprevalence of C. pneumoniae in elderly stroke and medical patients. Ninety-five incident cases of patients admitted consecutively with acute stroke or TIA, and 82 control subjects admitted concurrently with acute non-cardiopulmonary, non-infective disorders, were included in this study (both groups aged 65 years or older). Using commercial enzyme-linked immunosorbent assay (ELISA) kits, the presence of M. pneumoniae immunoglobulins IgA, IgG and IgM in patients' sera was determined. The seroprevalence of M. pneumoniae IgA, IgG and IgM in the stroke or TIA group (median age = 80) were 79, 61 and 6%, respectively. In the control group (median age = 80), the seroprevalence of respective M. pneumoniae IgA, IgG and IgM were 84, 50 and 11%. Using a logistic regression statistical model, adjusting for history of hypertension, smoking, diabetes mellitus, age and sex, history of ischaemic heart disease, and ischaemic electrocardiogram, the odds ratios of having a stroke or TIA in relation to M. pneumoniae IgA, IgG and IgM were 0.63 (95% confidence interval (CI) = 0.26-1.52, p = 0.31), 1.32 (95% CI = 0.66-2.64, p = 0.43) and 0.52 (95% CI = 0.14-1.92, p = 0.32), respectively. The study showed a high seroprevalence of M. pneumoniae in an elderly hospital population, using ELISA. Although the study ruled out M. pneumoniae seropositivity as a major risk factor for stroke in this elderly population, a smaller effect could not be excluded due to

  4. 血清C反应蛋白检测在小儿支原体肺炎感染中的临床价值%Value of C-reactive Protein Detection in Children with Mycoplasma Pneumonia Infection

    Institute of Scientific and Technical Information of China (English)

    左克

    2012-01-01

    Objective: To explore the diagnostic value of C-reactive protein ( CRP ) in infant with my-coplasma pneumonia. Method: The serum CRP levels were measured by nephelometric immunoassay and ELISA in 98 infants with mycoplasma pneumonia ( mycoplasma pneumonia group ) and 67 infants with bacterial pneumonia ( bacterial pneumonia group ) 72 healthy children ( control group ). Result: Mean CRP levels of the pneumonia group and bacterial pneumonia group on admission were signifcantly increased ( P mycoplasma pneumonia group ( P0. 05 ), however, the bacterial pneumonia group's CRP level was still higher than mycoplasma pneumonia group' s and the control group' s ( P< 0. 05 ); Among 115 childern whose CRP level were increased in admission, 68 children ( 59. 13% ) were younger than 4 years old, 47 children ( 40. 87% ) were 4 years old or older than 4, and the youngest of them was only 2 months. Conclusion: The CRP level for children with mycoplasma pneumonia is slightly higher than normal value, but lower than that of bacterial pneumonia. CRP can be used as an important auxiliary index of identification of mycoplasma pneumonia or bacterial pneumonia.%目的 探讨血清C反应蛋白检测在小儿支原体肺炎感染中的临床价值.方法 采用免疫比浊法测定98例支原体肺炎(支原体肺炎组)和67例细菌性肺炎患儿(细菌性肺炎组)血清 CRP,与 72例健康儿童(健康对照组)作对比.结果 入院时支原体肺炎组和细菌性肺炎组CRP均值和阳性率均显著高于对照组(P<0.05),且细菌性肺炎组显著高于支原体肺炎组(P<0.05);治疗1周后,两组CRP和阳性率指标较入院时均明显下降,支原体肺炎组与健康对照组比较无差别(P>0.05),但细菌性肺炎组仍高于支原体肺炎组和健康对照组(P<0.05);所有病例在入院时CRP和MP-IgM检测为阳性者,4岁以下儿童为59.13%(68/115),4岁以上为40.87%(47/115),其中年龄最小的仅生后2个月.结论 对于支原体肺炎患

  5. Clinical Significance of CRP and PCT Detection for the Diagnosis of Bacterial Pneumonia and Mycoplasma Pneumonia in Children%CRP及PCT检测对儿童细菌性肺炎及支原体肺炎诊断的临床意义

    Institute of Scientific and Technical Information of China (English)

    屠强

    2013-01-01

    目的:探讨C反应蛋白(CRP, C-reactive protein)及降钙素原(PCT, Procalcitonin)与儿童细菌性肺炎及支原体肺炎发病及病程的相关性。方法采用透射比浊法对140例儿童支原体肺炎及细菌性肺炎和70例健康受试者进行了检测。采用SPSS11.0软件包统计分析各组别间的差异性。结果细菌性肺炎组及支原体肺炎组的CRP水平均高于正常对照组,差异具有统计学意义(P<0.05)。细菌性肺炎组的CRP水平较支原体肺炎的CRP水平高(P<0.05)。细菌性肺炎及支原体肺炎组经治疗3d后与治疗前相比, CPR水平均显著性降低(P<0.05)。细菌性肺炎组的PCT水平高于正常对照组,有统计学意义(P<0.01)。此外,细菌性肺炎的PCT水平亦高于支原体肺炎组。治疗3d后细菌性肺炎组的PCT水平较支原体肺炎组高,两组之间有显著性差异(P<0.01)。结论 CRP对儿童细菌性肺炎和支原体肺炎反应性较好,可以作为临床诊断的依据;PCT对于细菌性肺炎有较好的指示作用,且对疾病的进程判断有参考价值。%Objective To investigate the correlation of C-reactive protein (CRP) and procalcitonin (PCT) level and diagnosis and prognosis of bacterial pneumonia and mycoplasma pneumonia in children.Methods Turbidimetric method was applied to detect the concentration of CRP and PCT in 140 cases children mycoplasma pneumonia and bacterial pneumonia respectively,and 70 healthy subjects were also enrolled for controls. SPSS11.0 software package Statistical was used to analysis the differences between groups. Results The results indicated that the CRP level in children with bacterial pneumonia or mycoplasma pneumonia was higher than the normal controls (P<0.05).CRP level in children with bacterial pneumonia group was higher than those with mycoplasma pneumonia (P<0.05).The CRP level in childeren accepted treatment for bacterial pneumonia or mycoplasma

  6. Contract Support and Facilitation of Epidemic Outbreak Surveillance (EOS) Program Final Operating Capability (FOC)

    Science.gov (United States)

    2007-08-01

    Chlamydophila pneumoniae MOMP Gene 232 bp Legionella pneumophila Macrophage Inf. Potentiator 160 bp Legionella micdadei Macrophage Inf. Potentiator 240 bp...developed and successfully demonstrated on the carbon electrode array for a set of pathogens including Mycoplasma pneumoniae, Chlamydophila pneumoniae

  7. Differential expression of lipoprotein genes in Mycoplasma pneumoniae after contact with human lung epithelial cells, and under oxidative and acidic stress

    Directory of Open Access Journals (Sweden)

    Tang Sen-Lin

    2008-07-01

    Full Text Available Abstract Background Mycoplasma pneumoniae is a human pathogen that is a common cause of community-acquired pneumonia. It harbours a large number of lipoprotein genes, most of which are of unknown function. Because of their location on the cell surface, these proteins are likely to be involved in the bacterial response to environmental changes, or in the initial stages of infection. The aim of this study was to determine if genes encoding surface lipoproteins are differentially expressed after contact with a human cell line, or after exposure to oxidative or acidic stress. Results Using qRT-PCR assays, we observed that the expression of a number of lipoprotein genes was up-regulated when M. pneumoniae was placed in contact with human cells. In contrast, lipoprotein expression was generally down-regulated or unchanged when exposed to either hydrogen peroxide or low pH (5.5. When exposed to low pH, the mRNA levels of four polycistronically transcribed genes in Lipoprotein Multigene Family 6 formed a gradient of decreasing quantity with increasing distance from a predicted promoter. Conclusion The demonstrated transcriptional changes provide evidence for the functionality of these mostly unassigned genes and indicate that they are regulated in response to changes in environmental conditions. In addition we have shown that the members of Lipoprotein Gene Family 6 may be expressed polycistronically.

  8. Research Progress in Diagnosis and Treatment of Childhood Mycoplasma Pneumoniae Infections%小儿肺炎支原体感染诊治研究进展

    Institute of Scientific and Technical Information of China (English)

    王军华

    2013-01-01

    肺炎支原体(MP)是小儿社区获得性肺炎常见的病原体之一,MP感染既有呼吸道症状,又可导致肺外多系统损害,血清特异性抗体检测依然是目前临床诊断MP感染的主要手段,聚合酶链反应技术可用于MP感染的早期诊断.大环内酯类仍是治疗小儿MP感染的首选抗生素,糖皮质激素及免疫球蛋白可用于重症MP感染,MP肺炎合并肺不张应争取在病程早期行纤维支气管镜灌洗治疗.%Mycoplasma pneumoniae( MP )in children is one of the common pathogens in community-acquired pneumonia. MP infection has both respiratory symptoms and extrapulmonary multi-system damages. Serum specific antibodies is still the primary means of clinical diagnosis of MP infection. Polymerase chain reac-tion( PCR) technology can be used for earlier diagnosis of MP infection. Macrolides is still the main antibiotic for the treatment. Corticosteroid and immunoglobulin can be used for intensive MP infection. MP pneumonia complicated with atelectasis should be treated flexible bronchoscopy lavage in the early stage of the disease as soon as possible.

  9. Mycoplasma hyorhinis is a potential pathogen of porcine respiratory disease complex that aggravates pneumonia caused by porcine reproductive and respiratory syndrome virus.

    Science.gov (United States)

    Lee, Jung-Ah; Oh, Yu-Ri; Hwang, Min-A; Lee, Joong-Bok; Park, Seung-Yong; Song, Chang-Seon; Choi, In-Soo; Lee, Sang-Won

    2016-09-01

    The porcine respiratory disease complex (PRDC) caused by numerous bacterial and viral agents has a great impact on pig industry worldwide. Although Mycoplasma hyorhinis (Mhr) has been frequently isolated from lung lesions from pigs with PRDC, the pathological importance of Mhr may have been underestimated. In this study, 383 serum samples obtained from seven herds with a history of PRDC were tested for specific antibodies to Mhr, Mycoplasma hyopneumoniae (Mhp), and porcine reproductive and respiratory syndrome virus (PRRSV). Seropositive rates of PRRSV were significantly correlated with those of Mhr (correlation coefficient, 0.862; P-value, 0.013), but not with those of Mhp (correlation coefficient, -0.555; P-value, 0.196). In vivo experiments demonstrated that pigs co-infected with Mhr and PRRSV induced more severe lung lesions than pigs infected with Mhr or PRRSV alone. These findings suggest that Mhr is closely associated with pneumonia caused by PRRSV and provide important information on Mhr pathogenesis within PRDC. Therefore, effective PRDC control strategies should also consider the potential impact of Mhr in the pathogenesis of PRDC. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. 分析小儿肺炎支原体肺炎的诊治效果%Effect of Diagnosis and Treatment of Pedo-mycoplasma Pneumonia

    Institute of Scientific and Technical Information of China (English)

    张瑛

    2015-01-01

    目的 探讨小儿肺炎支原体肺炎的诊治方式及效果. 方法 整群选取该院2013年10月—2014年10月收治的支原体肺炎患儿120例作为研究对象,对其临床资料进行回顾性分析. 所有患儿均采用颗粒凝集法对血清IgM抗体予以检测,按照诊治方法将所有患儿均分为对照组和观察组,各60例,其中对照组治疗药物为红霉素,观察组基于对照组加用阿奇霉素,对比两组临床疗效. 结果 对照组总有效率为80.0%,明显低于观察组91.7%,差异有统计学意义(P<0.05);对照组不良反应发生率为11.7%,明显高于观察组1.7%,差异有统计学意义(P<0.05). 结论 对于小儿肺炎支原体肺炎一定要尽快诊断并制定科学治疗方案,以控制病情,避免对小儿正常生长发育产生不利影响.该组经研究后发现阿奇霉素相较于红霉素治疗小儿支原体肺炎疗效更优,且并发症更少,值得推广.%Objective To investigate the treatment approach and effect of pedo-mycoplasma pneumonia. Methods 120 children with pedo-mycoplasma pneumonia admitted to our hospital from October 2013 to October 2014 were selected as study objects and their clinical data were retrospectively analyzed. All patients were given particle agglutination for detect-ing serum IgM antibody and were divided into observation group and control group according to diagnoses and treatment method, 60 cases in each group. The control group was given erythrocin and observation group was given azithromycin based on control group. The clinical effect of the two groups was compared. Results Total efficiency rate of control group was 80.0%, obviously lower than that of observation group (91.7%), and the difference was statistically significant (P<0.05); ad-verse reaction rate of control group was 11.7%, obviously higher than that of observation group (1.7%), and the difference was statistically significant (P<0.05). Conclusion Early Diagnosis should be done early and scientific

  11. Prediction of Genomic Islands in Three Bacterial Pathogens of Pneumonia

    Directory of Open Access Journals (Sweden)

    Wen Wei

    2012-03-01

    Full Text Available Pneumonia is one kind of common infectious disease, which is usually caused by bacteria, viruses, or fungi. In this paper, we predicted genomic islands in three bacterial pathogens of pneumonia. They are Chlamydophila pneumoniae, Mycoplasma pneumoniae and Streptococcus pneumoniae, respectively. For each pathogen, one clinical strain is involved. After implementing the cumulative GC profile combined with h and BCN index, eight genomic islands are found in three pathogens. Among them, six genomic islands are found to have mobility elements, which constitute a kind of conserved character of genomic islands, and this introduces the possibility that they are genuine genomic islands. The present results show that the cumulative GC profile when combined with h and BCN indexes is a good method for predicting genomic islands in bacteria and it has lower false positive rate than the SIGI method. Specially, three genomic islands are found to contain clusters of genes coding for production of virulence factors and this is useful for research into the pathogenicity of these pathogens and helpful for the treatment of diseases caused by them.

  12. Outbreak of Pneumonia in the Setting of Fatal Pneumococcal Meningitis among US Army Trainees: Potential Role of Chlamydia pneumoniae Infection

    Science.gov (United States)

    2011-06-02

    physical stress may contribute to an increased risk for infections with Streptococcus pneumoniae , Streptococcus pyogenes, Mycoplasma pneumoniae ...Chlamydia pneumoniae , Mycoplasma pneumoniae , Streptococcus pneumoniae , Bordetella pertussis, and Legionella pneumophila[10] in addition to undergoing...postexposure chemoprophylaxis. Mil Med 2003;168:1-6 7. Balicer RD, Zarka S, Levine H, et al. Control of Streptococcus pneumoniae serotype 5 epidemic of

  13. Epidemiological Investigation of Caprine Mycoplasma pneumoniae in Sichuan Province%山羊支原体性肺炎流行病学调查

    Institute of Scientific and Technical Information of China (English)

    王华; 杨发龙; 王永; 汤承

    2011-01-01

    山羊支原体性肺炎是威胁山羊养殖的重要传染病,为了解其流行情况,对四川省主要山羊养殖地区的山羊支原体性肺炎进行了流行病学调查.从四川省7个地区山羊养殖场采集肺脏和鼻腔棉拭子样本共135份,经过分离鉴定得到42株支原体,其中绵羊肺炎支原体36株,丝状支原体6株;其中6个羊场仅分离到绵羊肺炎支原体,1个羊场同时分离到绵羊肺炎支原体和丝状支原体.本试验结果表明,绵羊肺炎支原体是引起四川省山羊支原体性肺炎的主要病原,个别地方存在绵羊肺炎支原体和丝状支原体混合感染.%Caprine Mycoplasma pneumoniae (CMP) is one of the most serious threat to goat breeding in Sichuan province.The aim of this study was to investigate the prevalence of CMP. 135 clinical samples (lungs and nasal swabs) were collected from goat farms of 7 districts in Sichuan province and 42 clinical strains were isolated from these samples. The isolates were identified as M. ovipneumoniae (36/42) and the members of Mycoplasma mycoides cluster (6/42) by PCR. M. ovipneumoniae was isolated from all of 7 farms, and the members of Mycoplasma mycoides cluster was only isolated from one farm. The positive rate varied from 7.7% to 44.4% in 7 farms. The results suggested that M. ovipneumoniae was the major causative agent of CMP, and only a few cases was caused by both of M. ovipneumoniae and the members of Mycoplasma mycoides cluster in Sichuan province.

  14. Immunoactivity analysis of Cpn 0147 recombinant protein of Chlamydophila pneumoniae%肺炎嗜衣原体Cpn 0147重组蛋白的免疫学活性研究

    Institute of Scientific and Technical Information of China (English)

    代国知; 马忠夏; 周安文; 陈虹亮; 吴移谋

    2012-01-01

    目的 研究Cpn 0147重组蛋白的免疫学活性及其应用于肺炎嗜衣原体(Cpn) 感染临床诊断中的价值.方法 采用GST琼脂糖凝胶纯化目的 蛋白,将Cpn 0147重组蛋白免疫新西兰兔,分别应用Western blot、ELISA法检测其免疫反应性及免疫原性,同时通过检测临床标本以评估其诊断价值.结果 成功表达并纯化了相对分子质量约41×103的重组蛋白GST-Cpn 0147;Western blot和ELISA结果显示,该重组蛋白具有良好的免疫反应性,动物实验结果表明该重组蛋白具有较好的免疫原性,ELISA结果显示该重组蛋白免疫新西兰兔血清抗体效价为1∶12 800.结论 Cpn 0147重组蛋白具有较好免疫学活性和特异性,可为Cpn感染的确诊、预防研究奠定基础.%Objective To explore the immunoactivity of Cpn 0147 recombinant protein,and its clinical value for the diagnosis of Chlamydophila pneumoniae(Cpn) infection. Methods GST-Cpn 014 7 protein was purified by GST sepharose FF. Its immunoactivity was determined by Western blot, and clinical specimens were detected by enzyme linked immunosorbent assay(ELISA). Results The GST fusion protein with molecular weight of 41 × 103 was expressed. The purity of recombination protein after purification reached 95%. Both Western blot and ELISA showed good antigenicity of recombination protein. Conclusion The purified recombination protein might have high specificity and good immunological activity,which could lay a solid foundation for the further study on the serodiagnosis and prevention of Cpn.

  15. Mycoplasma pneumoniae Infections

    Science.gov (United States)

    ... Genitals and Urinary Tract Glands & Growth Head Neck & Nervous System Heart Infections Learning Disabilities Obesity Orthopedic Prevention Sexually Transmitted Skin Tobacco Treatments Injuries & ...

  16. C-反应蛋白检测在小儿细菌性肺炎与支原体肺炎中的临床比较%Clinical value of detection of C-reaction protein in diagnosis of pediatric bacterial pneumonia and Mycoplasma pneumonia:a comparative study

    Institute of Scientific and Technical Information of China (English)

    石丰月

    2013-01-01

    OBJECTIVE To study the clinical value of C-reactie protein (CRP) detection in diagnosis of the pediatric bacterial pneumonia and the Mycoplasma pneumonia so as to guide the diagnosis of infantile pneumonia.METHODS The children with bacterial pneumonia (the bacterial pneumonia group) and the children with Mycoplasma pneumonia (the Mycoplasma pneumonia group),who were treated in the pediatrics department from Sep 2010 to Sep 2012,were enrolled in the study,the healthy children receiving the medical examination (the control group)were also selected,with 80 cases in each group,then the change of CRP level was determined.RESULTS The CRP level of the control group was (3.24 ±0.45)mg/L,the bacterial pneumonia group before treatment (44.03±5.83) mg/L,the bacterial pneumonia group 3 days after the treatment (15.12±6.21) mg/L,the Mycoplasma pneumonia group before treatment (13.97±4.96) mg/L,the Mycoplasma pneumonia group 3 days after the treatment (5.29 ± 2.33) mg/L,the CRP level of the bacterial pneumonia group and the Mycoplasma pneumonia group before the treatment was significantly higher than that of the control group (P<0.05),the CRP level of the bacterial pneumonia group was higher than that of the Mycoplasma pneumonia group (P<0.05),the CRP level of the bacterial pneumonia group 3 days after treatment was higher than that of the Mycoplasma pneumonia group (P<0.05),the difference in the CRP level before and after the treatment between the bacterial pneumonia group and the Mycoplasma pneumonia group was statistically significant (P< 0.05).The positive rates of the CRP of the bacterial pneumonia group were 100.00% before the treatment and 52.50% 3 days after the treatment,which were respectively 66.25 % and 21.25 % in the Mycoplasma pneumonia group,the difference in the positive rate before and after the treatment between the bacterial pneumonia group and the Mycoplasmapneumonia group was statistically significant (P<0.05).CONCLUSION The positive

  17. Sensitivity of rabbit fibrochondrocytes to mycoplasmas

    Directory of Open Access Journals (Sweden)

    Nascimento Carlos Manuel de Oliveira

    2002-01-01

    Full Text Available Primary cell culture from rabbit meniscus (fibrochondrocytes-FcrC was infected for 24 hours with different inocula (10² to 10(7 Colony Forming Units-CFU of Mycoplasma hominis PG-21, M. pneumoniae FH and 1428 or M. arthritidis PG-6. The severity of the different obtained cytophatic effects-CPE was inoculum, Mycoplasma species and strain dependant. These bacteria were recovered from all infected FcrC and the SP4 medium for mycoplasmas also caused toxic effect on the FcrC. It was concluded that rabbit fibrochondrocytes were sensitive to mycoplasma infection, as well as to the SP4 mycoplasma medium.

  18. Detection and Significance of Chlamydophila Pneumonia Infection in Elder Chronic Bronchitis Patients%老年慢性支气管炎患者肺炎嗜衣原体感染的检测诊断150例分析

    Institute of Scientific and Technical Information of China (English)

    潘小成

    2013-01-01

    [ObjectiveJTo explore the detection method of chlamydophila pneumonia infection and its clinical significance. [Methods]75 cases of elderly patients with chronic bronchitis as the experimental group and 75 cases of elderly healthy patients as the control group. The CPN-IgG antibody and CPN MOMP amplification of the gene were detected by ELISA and PCR assay, respectively. [Results]The positive rates of CPN MOMP and CPN-IgG were 64.0% and 89.3%, respectively, significantly higher than the control group with the values of 12.0% and 25.3% . [Conclusion] ELISA and PCR methods for detection of CPN, can improve the diagnostic accuracy of the old chronic bronchitis.%[目的]探讨老年慢性支气管炎(chronic bronchitis,CB)患者血清中肺炎嗜衣原体(chlamydophila pneumonia,CPN)感染的检测方法及其临床意义.[方法]75例老年CB患者为实验组,75例健康老年志愿者为对照组;分别采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)和聚合酶链反应(polymerase chain reaction,PCR)法检测研究对象的肺炎嗜衣原体IgG(chlamydophila pneumonia-IgG,CPN-IgG)和主要外膜蛋白(major outer membmne protein,MOMP):CPN MOMP也称为外膜蛋白A(存在于Cpn原体和网状体,主要包括MOMP).实验组与对照组均用了上述两种方法.[结果]实验组CPN MOMP阳性、CPN-IgG阳性的构成比分别为64.0%和89.3%,均明显高于对照组的12.0%、25.3%,差异均具有统计学意义(P<0.05).结果显示,PCR和ELISA两种检测方法均能反映发生肺炎嗜衣原体感染.[结论]ELISA和PCR法均可对CPN进行检测,两种方法同时运用能提高老年CB的临床诊断率,降低假阴性的发生,利于改善预后.

  19. Analysis of the effect of glucocorticoid on the treatment of severe Mycoplasma pneumoniae pneumonia%糖皮质激素对重症肺炎支原体肺炎的治疗效果分析

    Institute of Scientific and Technical Information of China (English)

    何宇霞

    2016-01-01

    Objective:To investigate the effect of glucocorticoid in the treatment of severe Mycoplasma pneumoniae pneumonia. Methods:Clinical data were reviewed in our hospital in January 2010 ~ 2015 December 160 cases of severe mycoplasma pneumonia pneumonia patients, according to random number table method, divided into the treatment group(n=80) and control group(n=80), patients in control group received azithromycin was given conventional treatment, patients in the treatment group in the control group on the basis of be glucocorticoid treatment, compared two groups of patients with clinical curative effect. Results:Two groups of patients compared patients in the treatment group were cough time, cooling time, length of hospital stay were significantly shorter than that of the control group (P<0.05);the clinical therapeutic effect of two groups of patients compared treatment effective rate is 95% (76 cases) and the control of patients in the treatment group the effective rate was 70.00% (56 cases) and treatment effective rate was significantly higher than that of the control group (P < 0.05). Conclusion:In patients with severe pneumonia mycoplasma pneumonia be glucocorticoid combined with azithromycin treatment, not only can relieve clinical symptoms, but also can shorten the patient's clinical treatment time, to improve the treatment effect of patients, it is worth wide clinical application and promotion.%目的:探讨糖皮质激素治疗重症肺炎支原体肺炎的效果。方法:回顾我院2010年1月~2015年12月收治的160例重症肺炎支原体肺炎患者的临床资料,按照随机数字表法分为治疗组(n=80)和对照组(n=80),对照组予以阿奇霉素进行常规治疗,治疗组在对照组基础上予以糖皮质激素进行治疗,比较两组临床治疗效果。结果:治疗组咳嗽时间、退热时间、住院时间均明显短于对照组(P<0.05);两组临床治疗效果比较,治疗组治疗有效率为95.00%

  20. 小儿嗜肺军团菌肺炎合并肺炎支原体感染的临床特征分析%Clinical features of children's Legionella pneumophila pneumonia combine mycoplasma pneumoniae infection

    Institute of Scientific and Technical Information of China (English)

    陈小冰

    2014-01-01

    目的:探讨小儿嗜肺军团菌肺炎合并肺炎支原体感染的临床特征。方法回顾性分析2008年8月至2013年8月我院收治的50例嗜肺军团菌肺炎患儿的临床资料,其中单纯嗜肺军团菌感染患儿34例(对照组),合并肺炎支原体感染患儿16例(观察组),对两组患儿的临床特征进行对比分析。结果混合感染多见于城镇的学龄前儿童。在临床表现方面,观察组热峰高、热程长、肺部阳性体征、心率加快、颈部淋巴结肿大及肝脏增大的发生率明显高于对照组,住院时间明显长于对照组,差异具有统计学意义(P<0.05);在辅助检查方面,观察组胸片大片斑片影和胸腔积液,WBC增高、CRP增高,CK-MB增高、心电图阳性表现(包括心动过速/ST-T改变)、肝功能异常的发生率明显高于对照组,差异具有统计学意义(P<0.05)。结论小儿嗜肺军团菌肺炎合并肺炎支原体感染肺部症状体征重、易合并肺外器官损害,应积极的开展多中心、大样本的研究以获得更加可靠的资料,为临床防治提供帮助。%Objective To investigate the clinical features of children's Legionella pneumophila pneumonia combine mycoplasma pneumoniae infection. Methods Retrospectively analysis the clinical data of 50 cases of chil-dren with Legionella pneumophila pneumonia in our hospital from August 2008 to August 2013, 34 cases of children with simple Legionella pneumophila infection (control group), 16 cases of children combined mycoplasma pneumoniae infection (observation group), Analyzed and compared the clinical features of two groups . Results The mixed infec-tion seen more among urban preschool children. In terms of clinical manifestation, The thermal spike of observation group higher, thermal time longer than control group, the incidence of positive signs of lung, heart rate, cervical lymph node enlargement, and an increase of liver of observation group were

  1. Pneumonia

    Science.gov (United States)

    ... of pneumonia. Be sure to get the following vaccines: Flu vaccine can help prevent pneumonia caused by the flu virus. Pneumococcal vaccine lowers your chances of getting pneumonia from Streptococcus ...

  2. Pneumonia

    Science.gov (United States)

    ... overall health and whether it's caused by a virus or bacteria. With pneumonia caused by bacteria, a kid might ... tell if the infection is caused by a virus or bacteria. previous continue No More Pneumonia If the pneumonia ...

  3. The Effect of Vitamin A on Secretion of IFN-γ and IL-4 in A549 Cells Induced by Mycoplasma Pneumoniae

    Institute of Scientific and Technical Information of China (English)

    Xiaolan WU; Xianzhou LIU; Jilu TANG

    2008-01-01

    In order to investigate the effect of vitamin A (VA) on the secretion of IFN-γ and IL-4 in Mycoplasma Pneumoniae (MP)-induced A549 cells, A549 cells were co-cultured with MP for different time lengths and then the levels of IFN-γ and IL-4 in the cell culture supematants were detected before and after treatment with different concentrations of VA by using the enzyme-linked immunosorbent assay (ELISA). The results showed that the level of IFN-γ and IL-4 in the supernatants of MP-induced A549 cells was much higher than that in non-induced cells (P<0.01). After application of VA, IL-4 level was not increased until the concentration of VA was up to 0.5 × 10-5 mol/L (P<0.01).However, with concentration of VA increased up to 1 × 10-4 mol/L, IL-4 was significantly suppressed (P<0.01). It was concluded that MP could induce the secretion of IFN-y and IL-4 in A549 cells. VA could inhibit the secretion of IFN-γ, and increase the IL-4 level in MP-induced A549 cells. However,high concentration of VA had an inhibitory effect on the secretion of IL-4 as well as on the IFN-γ.These data provided a theoretical basis for the application of VA in MP pneumonia in the clinical practice.

  4. Curative Effect Research on Budesonide Inhalation in Treatment of Mycoplasma Pneumonia in Children%小儿支原体肺炎采用布地奈德雾化吸入法治疗疗效探究

    Institute of Scientific and Technical Information of China (English)

    陈秋琴

    2016-01-01

    Objective Mycoplasma pneumoniae pneumonia(MPP)is caused by mycoplasma pneumoniae infection. It is a clinical problem widely concerned by pediatricians,which takes up 10% to 40% of community-acquired pneumonia in children.MethodsThis experiment took 156 cases of children with mycoplasma pneumoniae pneumonia(MPP)who came to our hospital for treatment during April 2014 to April 2015 as the research object,and aerosol inhalation was applied on the basis of conventional treatment.Results This methods had high security,good effect,easy to operate,with less pain and good compliance.Conclusion Budesonide aerosol inhalation is suitable for patients at al ages.%目的:肺炎支原体(MPP)是由肺炎支原体感染所引起,占儿童社区获得性肺炎10%~40%,是儿科医师广泛关注的临床问题。方法本实验选择2014年4月~2015年4月来我院就诊的156例小儿支原体肺炎患者为研究对象,并以常规治疗为基础,以雾化吸入的方式进行相关治疗。结果该方法安全性高,效果好,痛苦少,患者依从性佳,操作简单。结论布地奈德雾化吸入法对于各个年龄段患者来讲均适合。

  5. Comparison of pyrosequencing, Sanger sequencing, and melting curve analysis for detection of low-frequency macrolide-resistant mycoplasma pneumoniae quasispecies in respiratory specimens.

    Science.gov (United States)

    Chan, Kwok-Hung; To, Kelvin K W; Chan, Betsy W K; Li, Clara P Y; Chiu, Susan S; Yuen, Kwok-Yung; Ho, Pak-Leung

    2013-08-01

    Macrolide-resistant Mycoplasma pneumoniae (MRMP) is emerging worldwide and has been associated with treatment failure. In this study, we used pyrosequencing to detect low-frequency MRMP quasispecies in respiratory specimens, and we compared the findings with those obtained by Sanger sequencing and SimpleProbe PCR coupled with a melting curve analysis (SimpleProbe PCR). Sanger sequencing, SimpleProbe PCR, and pyrosequencing were successfully performed for 96.7% (88/91), 96.7% (88/91), and 93.4% (85/91) of the M. pneumoniae-positive specimens, respectively. The A-to-G transition at position 2063 was the only mutation identified. Pyrosequencing identified A2063G MRMP quasispecies populations in 78.8% (67/88) of the specimens. Only 38.8% (26/67) of these specimens with the A2063G quasispecies detected by pyrosequencing were found to be A2063G quasispecies by Sanger sequencing or SimpleProbe PCR. The specimens that could be detected by SimpleProbe PCR and Sanger sequencing had higher frequencies of MRMP quasispecies (51% to 100%) than those that could not be detected by those two methods (1% to 44%). SimpleProbe PCR correctly categorized all specimens that were identified as wild type or mutant by Sanger sequencing. The clinical characteristics of the patients were not significantly different when they were grouped by the presence or absence of MRMP quasispecies, while patients with MRMP identified by Sanger sequencing more often required a switch from macrolides to an alternative M. pneumoniae-targeted therapy. The clinical significance of mutant quasispecies should be investigated further with larger patient populations and with specimens obtained before and after macrolide therapy.

  6. 肺炎嗜衣原体CPAF诱导THP-1细胞产生前炎症细胞因子和凋亡%Chlamydial protease-like activity factor from Chlamydophila pneumoniae induced THP-1 cells produced proinflammtory cytokines and apoptosis

    Institute of Scientific and Technical Information of China (English)

    胡旃; 吴移谋; 陈虹亮; 郑江花; 周洲; 唐国芳

    2010-01-01

    Objective To express and purify Chlamydial protease-like activity factor(CPAF)from Chlamydophila pneumoniae,for investigating the effect of its recombinant protein GST-CPAF in inducing human monocytic cells to secrete proinflammatory cytokines and cell apoptosis.Methods The recom-bination expression plasmid pGEX6p-2/CPAF from Chlamydophila pneumoniae was transformed into E.coli.The recombination GST-CPAF was expressed after induction by IPTG,and purified by a agarose gel FF.Human monocytic cells were stimulated by the GST-CPAF to test the production of tumor necrosis factor a(TNF-α)and interleukin-6(IL- 6)by ELISA.Inhibition of cells proliferation with GST-CPAF was assessed by MTT.The THP-1 cell apoptosis stimulated by GST-CPAF was detected by Hoechst33258 fluorescence staining,DNA fragmentation analysis and cell apeptosis was detested bv Annexin V-FITC-propidiuum iodide (PI)staining.Results The recombination protein GST-CPAF was successfully expressed with high level in E.coli,and stimulated human monocytic cells to produce proinflammatory cytokines including TNF-α and IL-6 in a dose-and time-dependent manner.Otherwise,the GST-CPAF inhibited the growth of human monocytic cell in a dose-dependent manner.Apoptosis with nuclear chromatin fragmentation as well as cell shrinkage was observed by fluorescent staining and microscopy,DNA ladders in apoptosis cells were detected after 24 h with the GST-CPAF.Conclusion The GST-CPAF from Chlamydophila pneumoniae can induce the secretion of proinflammatory cytokines TNF-α and IL-6 by human monocytic cells,and inhibited the proliferation of THP-1 cell and apoptosis in vitro.%目的 研究肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)衣原体蛋白酶样活性因子(Chlamydial protease-like activity factor,CPAF)能否在体外诱导人单核细胞THP-1产生前炎症细胞因子和凋亡,为进一步探索Cpn感染宿主致病的分子机制提供实验依据.方法 将Cpn CPAF全基因克隆于pGEX6p-2

  7. Experimentally produced calf pneumonia.

    Science.gov (United States)

    Gourlay, R N; Howard, C J; Thomas, L H; Stott, E J

    1976-03-01

    Experimental pneumonia was produced in calves by the endobronchial inoculation of pneumonic lung homogenates. Irradiated homogenates produced minimal pneumonia. Ampicillin treatment of the homogenates and the experimental calves reduced the extent of pneumonia. Treatment with tylosin tartrate prevented experimental pneumonia. These results suggest that the total pneumonia was due to organisms susceptible to tylosin tartrate and that the residual pneumonia remaining after ampicillin treatment was due to organisms susceptible to tylosin tartrate but not to ampicillin. Of the organisms isolated from the lungs, the ones in this latter category most likely to be responsible are Mycoplasma dispar and ureaplasmas (T-mycoplasmas).

  8. 两种抗肺炎支原体抗体检测方法比较%Comparison of two anti-mycoplasma pneumonia antibody detection methods

    Institute of Scientific and Technical Information of China (English)

    孙炜; 赵勇

    2013-01-01

    Objective:To explore the sensitive and specific methods to be used in the early diagnosis of Mycoplasma pneumonia. Methods:Separately used the Colloidal Gold and the SERODIA-MYCOⅡpassive agglutination method in the examination of MP antibody in the blood sera of 77 children hospitalized for respiratory infections, and compared the diagnostic specificity and sensitivity of the tow methods.Results:The sensitivity of the Colloidal Gold and the SERODIA-MYCOⅡpassive agglutination are 92.3%and 97.4%respectively and the specificity are 97.4%and 94.7%respectively;the difference between them are not statistically significant (P>0.05). Conclusions:Both the Colloidal Gold and the SERODIA-MYCOⅡpassive agglutination method can be used in the early diagnosis of Mycoplasma pneumonia, but the Colloidal Gold method is more rapid and cheap.%目的:探讨早期诊断肺炎支原体(MP)感染的实验室检测方法。方法:分别采用胶体金法和被动凝集法检测77例呼吸道感染住院患儿血清中的抗肺炎支原体抗体,并对两种方法的诊断特异度和灵敏度进行比较。结果:胶体金法和被动凝集法的诊断灵敏度分别为92.3%和97.4%,诊断特异度分别为97.4%和94.7%;二者检测结果的差异无统计学意义(P>0.05)。结论:胶体金法和被动凝集法均为支原体肺炎早期诊断较理想的方法,但前者的试验方法更为快速且成本更为低廉。

  9. Value of CT angiography in the diagnosis of mycoplasma pneumonia and image analysis%CT造影在诊断支原体肺炎中的价值及影像学特点分析

    Institute of Scientific and Technical Information of China (English)

    张华

    2015-01-01

    目的 探讨CT造影成像技术在支原体肺炎诊断中的临床应用价值,分析支原体肺炎CT造影成像的影像学特点.方法 选择经实验室血清检查确认为支原体肺炎的患者34例为研究对象,对其进行CT造影扫描,周迭代法重构图像,分析其影像学特点对支原体肺炎的诊断价值.结果 支原体肺炎发病女性明显高于男性,差异有统计学意义(x2=9.529,P<0.05);支原体肺炎40岁或40岁以下者发病明显多于40岁以上者,差异有统计学意义(x2=11.765,P<0.01);有33例患者CT影像上可见到磨玻璃密度阴影,占97.06%,其影像学特点表现为由磨玻璃影逐渐过度到大片实变影、在磨玻璃影像中散在分布多发斑片状实变影、以上两种改变同时存在、在磨玻璃影像中散在分布结节影等;胸腔积液14例,占41.18%;纵隔淋巴结增大10例,占29.41%;女性发病明显高于男性(P<0.05);左下叶发病明显多于其他叶段(P<0.01).结论 肺部CT造影影像特点对支原体肺炎的诊断具有重要的提示意义.%Objective To investigate the clinical value of CT imaging technique in the diagnosis of mycoplasma pneumonia, and analyze the CT imaging characteristics of mycoplasma pneumonia.Methods Thirty-four patients with mycoplasma pneumonia diagnosed by laboratory serum examination were selected.The patients had contrast-enhanced CT scan using iterative method for reconstructed image, the the clinical value of imaging in the diagnosis of Mycoplasma pneumonia was analyzed.Results The incidence of mycoplasma pneumonia in women was more than that in men, the difference was significant (x2 =9.529, P < 0.05);Patients with mycoplasma pneumonia 40 and below 40 years old were more than patients with more than 40 years old, the difference was significant (x2-11.765, P < 0.01);the CT image in 33 cases showed visible ground glass density shadow, accounting for 97.06% (33/34), its image characteristics from consolidation

  10. The curative efficacy observation of erythromycin and ultrashort wave in the treatment of 40 cases of children’s lobar pneumonia caused by mycoplasma pneumonia%红霉素联合肺部超短波治疗肺炎支原体引起的儿童大叶性肺炎40例疗效观察

    Institute of Scientific and Technical Information of China (English)

    吴平; 刘娥

    2014-01-01

    Objective:To explore the clinical curative efficacy of erythromycin combined with ultrashort wave in the treatment of children’s lobar pneumonia caused by mycoplasma pneumonia. Methods:40 cases of children’s lobar pneumonia in our hospital caused by mycoplasma pneumonia wer e analyzed r etrospectively. Results: This group of children’s mycoplasmal pneum onia was char acterized by fever, intense paroxysmal cough and wheezing. Meanwhile, the image showed lobar pneumonia. Erythromycin and ultrashort wave therapy is effective. Conclusion:For children’s lobar pneumonia caused by mycoplasma pneumonia, remarkable curative effect can be achieved by the appropriate treatment.%目的:探讨红霉素联合肺部超短波对肺炎支原体引起的儿童大叶性肺炎的临床疗效。方法:采用回顾性方法对本院收治的40例儿童肺炎支原体引起的大叶性肺炎病例进行分析。结果:本组儿童支原体肺炎以发热、阵发性剧咳、喘息为主,影像学显示大叶性肺炎。红霉素联合肺部超短波治疗有效。结论:肺炎支原体引起的大叶性肺炎,经合理治疗可以取得显著疗效。

  11. 儿童肺炎支原体感染的检验结果探究%Test results of Mycoplasma pneumoniae infection in children

    Institute of Scientific and Technical Information of China (English)

    范昂

    2014-01-01

    Objective To investigate the infection of mycoplasma pneumonia in children's test results. Methods Randomly select-ed in 2011 October-2012 year in November to the clinical symptoms of 50 cases in our hospital were fever, cough and respiratory tract infection symptoms in children, using ELISA to detect serum MP-IgM, and blood cell analysis and blood biochemistry, blood and urine routine and other inspection. Results 50 patients were examined after most of the children, the white cell count and platelet count were under normal or high range, 15 cases were MP-IgM positive, 32 cases were cold agglutination test showed pos-itive, 10 cases were the result of urine routine test is abnormal, 23 cases of patients with abnormal blood gas, 6 cases of patients with alanine transaminase appeared significantly increased, myocardial enzyme is high, 30 patients in addition, sputum culture Klebsiella pneumoniae, Streptococcus pneumoniae and Enterobacter cloacae. Conclusion To give children were detected My-coplasma pneumoniae infection, can effectively improve the correctness of diagnosis, is of great significance for the treatment of children, worthy of promotion.%目的:探讨儿童肺炎支原体感染的检验结果。方法随机选择2011年10月-2012年11月到我院就诊的50例临床症状为发热、咳嗽以及呼吸道感染等症状的儿童,使用酶联免疫对血清中的MP-IgM进行检测,并进行血细胞分析以及血生化、血尿常规等各项检查。结果50例患儿经过检测过后,大部分患儿的白细胞数量与血小板计数均处于正常或是偏高范围,15例患儿的MP-IgM为阳性,32例患儿经过冷凝集试验呈现出阳性,10例患者尿常规检查结果为异常,23例患者血气异常,6例患者丙氨酸转氨酶出现明显升高,30例患者的心肌酶偏高,另外,痰培养出肺炎克雷伯菌、肺炎链球菌以及阴沟肠杆菌。结论给予患儿进行肺炎支原体感染检测,可有效提

  12. 小儿肺炎支原体感染后血常规分析的临床探索%The Clinical Study on Blood Routine Examination in the Children With Mycoplasma Pneumoniae Infection

    Institute of Scientific and Technical Information of China (English)

    陈庆; 罗欣

    2016-01-01

    ObjectiveThe blood routine examination is discussed in the treatment of infantile pneumonia mycoplasma infection clinical effect.MethodsSelected from April 2011 to March 2015,65 cases of children mycoplasma infection. Then selected the same clinic examination 65 cases of children,compared to the routine blood test results,the differences were analyzed.Results Children with mycoplasma infection white blood cel count increased, neutrophils ratio and absolute value increased,the proportion of lymphocytes increased and the decline in absolute value,monocyte percentage and absolute value,comparison results between the groups had statisticaly significant difference(P0.05).Conclusion Routine blood test results is helpful to distinguish whether the pediatric pneumonia by mycoplasma pneumonia infection,for the diagnosis of children pediatric pneumonia mycoplasma infection to provide reliable decision basis.%目的:探讨分析血常规检查分析在小儿肺炎支原体感染治疗中的临床作用。方法选取我院2011年4月~2015年3月儿科收治入院的支原体感染患儿65例,再选取同期门诊检查患儿65例对比其血常规检查结果,分析两者区别。结果支原体感染患儿白细胞计数增高、中性粒细胞比例及绝对值增高、淋巴细胞比例及绝对值下降、单核细胞比例及绝对值增高,两组患者对比结果差异有统计学意义(P<0.05)。红细胞计数、血红蛋白含量和血小板计数差异无统计学意义(P>0.05)。结论血常规检查结果有利于分辨小儿所患肺炎是否为支原体感染肺炎,对于小儿肺炎支原体感染患儿的确诊提供可靠的判定依据。

  13. Potential role of tigecycline in the treatment of community-acquired bacterial pneumonia

    Science.gov (United States)

    Townsend, Mary L; Pound, Melanie W; Drew, Richard H

    2011-01-01

    Tigecycline is a member of the glycylcycline class of antimicrobials, which is structurally similar to the tetracycline class. It demonstrates potent in vitro activity against causative pathogens that are most frequently isolated in patients with community-acquired bacterial pneumonia (CABP), including (but not limited to) Streptococcus pneumoniae (both penicillin-sensitive and -resistant strains), Haemophilus influenzae and Moraxella catarrhalis (including β-lactamase-producing strains), Klebsiella pneumoniae, and ‘atypical organisms’ (namely Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila). Comparative randomized clinical trials to date performed in hospitalized patients receiving tigecycline 100 mg intravenous (IV) × 1 and then 50 mg IV twice daily thereafter have demonstrated efficacy and safety comparable to the comparator agent. Major adverse effects were primarily gastrointestinal in nature. Tigecycline represents a parenteral monotherapy option in hospitalized patients with CABP (especially in patients unable to receive respiratory fluoroquinolones). However, alternate and/or additional therapies should be considered in patients with more severe forms of CABP in light of recent data of increased mortality in patients receiving tigecycline for other types of severe infection. PMID:21694911

  14. 肺炎支原体感染现状及其与哮喘的关系%Mycoplasma pneumoniae infection and its relationship with asthma

    Institute of Scientific and Technical Information of China (English)

    冯金燕; 陈志敏

    2010-01-01

    Mycoplasma pneumoniae(MP) is one of the most important pathogens for respiratory tract infections in older children and adults. And it also has a close relationship with the development, acute exacerbation and persistence of chronic difficult-to-control symptoms of asthma.But so far,the mechanisms inducing or causing asthma remain unclear.Possible mechanisms include direct damage of bronchial mucosa, IgE-mediatad immune response, a variety of cytokines and inflammatory mediators releasing and the propensity of host.%肺炎支原体(MP)是年长儿童与成年人呼吸道感染的重要病原之一,且与哮喘的发生、急性恶化发作及慢性症状难以控制密切相关.但迄今为止,MP感染诱发或引起哮喘的机制仍不清楚,可能与MP对支气管黏膜的直接损伤作用、IgE介导的免疫反应、各种细胞因子和炎症介质的释放及宿主体质有关.

  15. Advances in Carbon Metabolism in Mycoplasma Pneumoniae%肺炎支原体碳源代谢的研究进展

    Institute of Scientific and Technical Information of China (English)

    曹阳; 张树明

    2014-01-01

    Mycoplasma pneumoniae as small genome, its metabolic network includes 129 enzyme catalytic completed 189, so its synthesis and catabolism of ability is poor, mainly from the outside world to absorb nutrients. They need to grow in in vitro culture medium contains 19 kinds of essential nutrients.Through the recent years, the research results show that MP metabolic processes, met-abolic processes play a key role in a variety of functions and the role of the enzyme metabolites.%肺炎支原体(M. pneumonia,Mp)由于基因组小,其代谢网络包括了由129个酶催化完成的189个反应,因此其合成和分解代谢的能力差,生长所需的营养成分主要从外界摄取,在体外培养时培养基中包含了19种基本营养物质。本文通过近年来,对MP代谢过程的研究结果表明,代谢过程中起到关键作用的酶具有多种功能以及代谢产物的作用。

  16. 哮喘患儿肺炎衣原体及肺炎支原体感染的临床特征分析%Clinical characteristics of Chlamydia pneumoniae and Mycoplasma pneumoniae infections in children with asthma

    Institute of Scientific and Technical Information of China (English)

    王爱虹; 王景刚; 宋永辉

    2015-01-01

    目的:观察分析哮喘急性发作患儿肺炎衣原体及肺炎支原体感染的临床特征,以降低哮喘患儿医院感染率。方法选取2012年3月-2013年3月儿科收治的哮喘急性发作患儿共180例,统计分析患儿哮喘病程长短与肺炎衣原体感染和肺炎支原体感染的关系及其药敏检测结果。结果哮喘急性发作患儿肺炎支原体感染22.2%、肺炎衣原体感染22.8%;其中哮喘病程≤3个月的患儿中肺炎衣原体的感染率为41.0%,比哮喘病程3~6个月和>6个月的患儿感染率高,3者比较差异有统计学意义(P<0.05);而肺炎支原体在哮喘病程>6个月的患儿中感染率最高,为38.3%;患儿年龄≤3岁的肺炎衣原体和支原体的感染率分别为39.6%和33.3%,均高于年龄3~6、>6岁患儿;肺炎衣原体对红霉素耐药率最低,为17.1%,对四环素耐药率最高,为78.0%;肺炎支原体对红霉素耐药率最低,为22.5%,对庆大霉素耐药率最高,为77.5%。结论肺炎衣原体和肺炎支原体感染是导致哮喘急性发作的主要病原体,其中患儿≤3岁,哮喘病程≤3个月和>6个月的患儿感染肺炎支原体和衣原体的概率较高。%OBJECTIVE To observe the clinical characteristics of Chlamydia pneumoniae and Mycoplasma pneu‐moniae infections in the children with acute exacerbation of asthma so as to reduce the incidence of nosocomial in‐fections in the children with asthma .METHODS A total of 180 children with acute exacerbation of asthma ,who were treated in the department of pediatrics from Mar 2012 to Mar 2013 ,were enrolled in the study ;the relation‐ship between the course of asthma and the C .pneumoniae and M .pneumoniae infections was observed ,and the re‐sults of drug susceptibility testing for the C .pneumoniae and M .pneumoniae were statistically analyzed .RESULTS The incidence of M .pneumoniae infection was 22

  17. Lack of effect of aerial ammonia on atrophic rhinitis and pneumonia induced by Mycoplasma hyopneumoniae and toxigenic Pasteurella multocida

    DEFF Research Database (Denmark)

    Andreasen, Morten; Bækbo, P.; Nielsen, Jens

    2000-01-01

    The objective of this experimental study was to determine the effects of aerial ammonia on disease development and bacterial colonization in weaned pigs inoculated with toxigenic Pasteurella multocida and Mycoplasma hyopneumoniae. Two groups of 10 pigs each were continuously exposed to 50 and 100 p......, tonsils, lungs and kidneys, or the average daily weight gain. The recovery of toxigenic P. multocida from nasal swabs following inoculation was significantly greater in pigs exposed to 50 p.p.m. ammonia or more as compared to the control group. In conclusion, high levels of ammonia combined...... with inoculations with M. hyopneumoniae and toxigenic P. multocida had no significant effect on disease development, but may have enhanced colonization by toxigenic P. multocida on the nasal turbinates....

  18. 肺炎衣原体、支原体在哮喘致病机制中的作用%Role of Chlamydophila pneumoniae and Mycoplasma pneumoniae in asthma pathogenesis

    Institute of Scientific and Technical Information of China (English)

    赵睿; 戚好文

    2007-01-01

    肺炎衣原体、支原体在哮喘致病机制中的作用引起了国际学者极大的兴趣.随着对肺炎衣原体、支原体与哮喘相关性认识程度的加深,结合众多学者所做的富有成效的研究成果,现将肺炎衣原体、支原体感染在哮喘致病机制中作用的研究进展作一综述.

  19. Observation of curative effect by azithromycin combined with Tanreqing in the treatment of 64 pediatric mycoplasma pneumoniae pneumonia cases%观察阿奇霉素联合痰热清治疗小儿肺炎支原体肺炎64例的疗效

    Institute of Scientific and Technical Information of China (English)

    李文华

    2015-01-01

    目的:评价对肺炎支原体肺炎患儿联合使用阿奇霉素和痰热清的治疗疗效。方法128例肺炎支原体肺炎患儿随机分成对照组和研究组,各64例,对照组用阿奇霉素治疗,研究组联合用阿奇霉素和痰热清治疗,分析两组的治疗疗效。结果研究组总有效率为95.31%,对照组总有效率为78.13%,差异有统计学意义(P<0.05)。结论对肺炎支原体肺炎患儿联合使用阿奇霉素和痰热清治疗效果显著,有推广价值。%Objective To evaluate curative effect by azithromycin combined with Tanreqing in the treatment of pediatric mycoplasma pneumoniae pneumonia. Methods A total of 128 children with mycoplasma pneumoniae pneumonia were randomly divided into control group and research group, with 64 cases in each group. The control group received azithromycin for treatment, and the research group received combination of azithromycin and Tanreqing for treatment. Curative effects of the two groups were compared. Results Total effective rate was 95.31% in the research group, and 78.13% in the control group. Their difference had statistical significance (P<0.05). Conclusion Implement of azithromycin combined with Tanreqing provides precise effect in treating pediatric mycoplasma pneumoniae pneumonia, and it contains value for promotion.

  20. Misdiagnosis Literature and Analysis of 282 Cases with Mycoplasma Pneumoniae Infection%肺炎支原体感染282例误诊文献分析

    Institute of Scientific and Technical Information of China (English)

    黄秀娜; 马政辉; 王露霞; 石玉玲

    2012-01-01

    目的 探讨肺炎支原体( mycoplasma pneumonia,MP)感染的误诊原因,以提高诊断率.方法 检索2007年1月-2011年12月中国期刊全文数据库、维普数据库、万方医学数据库中MP感染误诊相关文献,对命中的11篇282例MP感染误诊病例的临床资料进行复习,分析误诊原因.结果 282例均以发热、呼吸道症状为主要表现,部分患者有循环、消化、神经、泌尿及血液系统等多种表现.误诊疾病达20余种,从多到少依次为细菌或病毒性肺炎、上呼吸道感染、病毒性心肌炎、病毒性脑炎、支气管炎、急性胃肠炎、肺结核、败血症、贫血、肾炎、伤寒、腹泻原因待查、病毒性肝炎、类风湿关节炎、肾病综合征、自身免疫性溶血性贫血、过敏性紫癜、荨麻疹、急性淋巴结炎、胆囊炎及猩红热,误诊时间3d~6个月,中位误诊时间11d.所有病例按误诊疾病治疗效果差,完善相关实验室检查或行诊断性治疗后确诊为MP感染,经大环内酯类药物治疗痊愈.11篇文献中5篇报道误诊率,为20.0%~64.3%,平均误诊率为24.9%.结论 MP感染既可能被误诊为由细菌、病毒、结核菌等病原体引发的感染,也可能被误诊为某些免疫性疾病.掌握正确诊断思维方法、重视实验室检查、施以规范诊断性治疗,有助于减少误诊.%Objective To investigate the misdiagnosis causes of mycoplasma pneumoniae (MP) infection in order to improve the diagnostic rate. Methods Misdiagnosis literatures of mycoplasma pneumoniae infection during January 2007 and December 2011 in China Journal Full text Database (CJFD), CQV1P Database and Wanfang Medicine Database were searched. We sifted 11 pieces of related clinical data for review, which included 282 cases with MP infection, and analyzed the misdiagnosis cause. Results Fever and respiratory symptoms were the main clinical manifestations in the 282 cases, some of which had clinical manifestations in

  1. Clinical analysis of 12 cases of severe mycoplasma pneumonia of children%12例儿童难治性支原体肺炎临床分析

    Institute of Scientific and Technical Information of China (English)

    张艳萍

    2013-01-01

    Objective To explore the diagnosis and treatment of severe mycoplasma pneumonia of children. Methods Clinical manifestations,diagnosis and treatment of 12 cases of severe mycoplasma pneumonia of children were reviewed. Results 12 chilidren were 3-12 years old, coughed in variety. 7 cases had been fevering for more than 10 days, 4 cases less than 10 days and 1 cases no fever.Lung CT, one case menifested bronchitis, 4 cases bronchial pneumonia and 7 cases consolidation of lobi pulmonis .5 cases had smal -medium pleural effusion and 1 case a great deal .Peripheral white blood cel counts of 9 cases rised and 3 cases were normal . 2 cases had normal CRP and 10 cases rised. 1 case was complicated by moderate anemia. 7 cases Myocardial enzyme and 2 cases glutamic-pyruvic transaminase rised .1 case urine protein was positive. 12 patients with macrolides antibiotics were more than 7days , and 8 cases cephalosporin antibiotics were combined . 7 cases methyl prednisolones were applied. 3 patients received gamma globulin, ang one of them received blood plasma and packed red cel s. 3 severe patients were transferred to other hospitals.At the end pulmonary fibrosis was formed in one of them. Conclusions The ages of most of the patients with refractory mycoplasma pneumonia are elder. These cases may develop severe,or be combined with other infections and damages of other organs. We should be aware at the erlier time .If necessary, adrenal cortex hormone, gamma globulin and other antibiotics can be applied.%目的探讨难治支原体肺炎的临床表现、诊断依据和治疗方法。方法回顾12例难治和重症支原体肺炎的临床表现、诊疗经过,查阅相关文献进行分析讨论。结果12例患儿年龄3-12岁,年龄偏大,咳嗽程度不等;反复发热持续时间大于10天7例,小于10天4例,无发热1例;肺部CT,1例表现支气管炎,4例支气管肺炎,7例显示程度不等的肺叶实变,其中2例累及双肺,1

  2. C-反应蛋白检测在小儿细菌性肺炎及支原体肺炎中的应用%Detection of C-reactive protein applied in diagnosis of children with bacterial pneumonia and mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    贺箭飞

    2012-01-01

    目的 对比分析C反应蛋白检测在小儿细菌性肺炎和支原体肺炎的诊断价值.方法 选取148例肺炎患儿及同期进行健康体检的150例健康小儿作为研究对象,对所有受试者进行C-反应蛋白、肺炎支原体、血常规检测,对148例肺炎患儿治疗前后进行C-反应蛋白水平检测,比较健康小儿、支原体肺炎患儿、细菌性肺炎患儿的C-反应蛋白水平,比较细菌性肺炎患儿与支原体肺炎患儿治疗前后的C-反应蛋白水平,比较C-反应蛋白对不同类型肺炎患儿的阳性检测率,综合评价C-反应蛋白在肺炎患儿诊断鉴别中的价值.结果 健康患儿、肺炎支原体患儿、细菌性肺炎患儿的C反应蛋白分别为(3.58±0.79)、(14.82±3.69)、(68.54±28.31)mg/L;组间比较,肺炎组患儿明显高于健康组患儿(P<0.05),而细菌性肺炎组患儿明显高于支原体肺炎组患儿(P<0.05);细菌性肺炎患儿C-反应蛋白的阳性率为93.3%、支原体肺炎患儿为45.6%,患儿经治疗后,C-反应蛋白均明显下降,上述指标组间比较及治疗前后比较,差异均有统计学意义(P<0.05).结论 C-反应蛋白检测可作为鉴别细菌性肺炎和支原体肺炎的有效手段,有助于临床医师采取相应的治疗方法及早治疗患儿,以免耽误病情.%OBJECTIVE To compare and analyze the C-reactive protein detection for the diagnosis of the children with bacterial pneumonia and mycoplasma pneumonia. METHODS A total of 148 children with pneumonia and 150 healthy children were chosen as the research subjects. All subjects were taken with the C-reactive protein, Mycoplasma pneumoniae. and blood testing. The levels of C-reactive protein of 148 cases of pneumonia children were tested before and after the treatment. The C-reactive protein levels of the healthy children, the children with mycoplasma pneumonia, and the children with bacterial pneumonia were compared. The C-reactive protein levels of the children

  3. The Mutation Analysis of Mycoplasma Pneumoniae Infection Children’s Blood Routine Indexes and Hs-CRP%肺炎支原体感染患儿血常规、超敏C反应蛋白变化分析

    Institute of Scientific and Technical Information of China (English)

    邢梅芳

    2014-01-01

    Objective:To observe Mycoplasma pneumoniae infects the change to children’s blood routine indexes and hs-CRP,and analyze Mycoplasma pneumoniae infects the influence to children’s blood routine indexes and hs-CRP. Method:The infection group:78 Mycoplasma pneumoniae infects;control group:86 normal children,were used to detect the children’s blood routine indexes and hs-CRP and makes a comparative analysis. Result:Mycoplasma pneumoniae infection group neutrophil proportion and absolute value increased,lymphocyte proportion and absolute values decreased,monocyte proportion and absolute value increased,platelet count increased(P0.05). Conclusion:Combined with changes of childhood’s C-reactive protein and blood routine to infer the possibility of MP infection.%目的:观察肺炎支原体感染患儿的血常规及超敏C反应蛋白结果的变化,分析肺炎支原体感染对儿童血常规及超敏C反应蛋白的影响。方法:对164例儿童,其中感染组:肺炎支原体抗体检测阳性患儿78例;对照组:同期86例正常体检儿童,分别做血常规及超敏C反应蛋白检测并进行对比分析。结果:肺炎支原体感染组中性粒细胞比例及绝对值增高,淋巴细胞比例及绝对值下降,单核细胞比例及绝对值增高,血小板计数增高,比较差异有统计学意义(P<0.01);两组超敏C反应蛋白、白细胞计数及嗜酸性粒细胞比例及绝对值和嗜碱性粒细胞比例及绝对值、红细胞计数、血红蛋白含量均无明显变化。结论:结合儿童超敏C反应蛋白及血常规的变化有助于推断肺炎支原感染可能性。

  4. 241例儿童支原体肺炎临床诊断和治疗%Analysis of clinical diagnosis and treatment of 241 cases of child mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    杨膺黄; 倪馨莉; 宋善路

    2013-01-01

    Objective:The analysis of the clinical characteristics of people with mycoplasma pneumonia, imaging findings and treatment.Methods:A retrospective analysis of 241 cases diagnosed as Mycoplasma pneumoniae pneumonia in children in our hospital's clinical medical records.Results:Mycoplasma pneumoniae pneumonia clinical symptoms such as cough and fever;nfants and young children are usual y accompanied by shortness of breath, wheezing, auscultation could be heard and sputum.Showing the diversity of imaging features,Patchy shadow, common in infants and young children.Segmental, large patchy shadows, more common in school-age children.Erythromycin or azithromycin in the treatment available to the clinical efficacy;Part of the refractory Mycoplasma pneumoniae,Given the combination of glucocorticoids in the drug treatment at the same time,can stil get a good clinical efficacy.Conclusion:Mycoplasma pneumoniae has the characteristics of clinical presentation and imaging findings do not match,the imaging findings of a lack of specificity.Early specification of application macrolides timely treatment, which can effectively al eviate the clinical manifestations, and prevent the recurrence of a very important clinical significance.%目的:分析患有肺炎支原体肺炎患儿的临床特点、影像学表现与治疗手段。方法:回顾性分析241例确诊为肺炎支原体肺炎在我院住院的患儿的临床病历资料。结果:肺炎支原体肺炎主要的临床症状为咳嗽及发热等;婴幼儿通常还伴有气短喘憋、听诊可闻及痰鸣音。影像学特征呈现多样性,主要为斑片状阴影时,以婴幼儿常见,主要为节段性、大片状阴影时,以学龄儿童多见。应用红霉素或者阿奇霉素治疗可获得很好的临床疗效;部分难治性支原体肺炎,在药物治疗同时联合应用糖皮质激素,仍可得到不错的临床疗效。结论:支原体肺炎具有临床表现和影像学表现不相符

  5. Clinical analysis on children with extrapulmonary damage as the first symptoms of Mycoplasma pneumoniae infection%小儿以肺外损害为首发症状的肺炎支原体感染临床分析

    Institute of Scientific and Technical Information of China (English)

    吴国媛; 卓俊瑞

    2015-01-01

    Objective:to investigate the clinical manifestations of pediatric extrapulmonary organ damage as first symp-toms of Mycoplasma pneumonia(MP)infection,improve the diagnostic and treatment level. Methods:The clinical data of 79 children with Mycoplasma pneumonia infection of extrapulmonary organ damage as first symptoms were analyzed retro-spectively. Results:Among 578 children with Mycoplasma pneumoniae infection,79 children were found with extrapulmo-nary organ damage as starting symptoms(13. 7% ),79 children involving the main organs included hematologic system (28children,35. 4% ),nervous system( 19children,24. 1% ),skin lesion( 11 children,13. 9% ),digestive system( 7children,8. 9% ),cardiovascular system( 6children,7. 6% ),connective tissue(5children,6. 3% ),muscular and joint damage(2children,2. 5% ),and urinary system( 1children,1. 3% ). Conclusion:Damage of other visceral organs is fre-quently observed in children with Mycoplasma infection . Children with respiratory infection and multi - organs disfunction should not overlook the complications caused by Mycoplasma pneumoniae infection ,especially when the symptoms of respir-atory tract infection appear.%目的:分析小儿以肺外脏器损害为首发症状的肺炎支原体(Mycoplasma pneumoniae ,MP)感染的临床表现,提高诊治水平。方法回顾分析以肺外脏器损害为首发症状的79例 MP 感染患儿临床资料。结果肺炎支原体(MP)感染578例中79例以肺外器官损害为首发症状(13.7%),累及的主要器官有血液系统28例(35.4%)、神经系统19例(24.1%)、皮肤损害11例(13.9%)、消化系统7例(8.9%)、心血管系统6例(7.6%)、结缔组织5例(6.3%)、肌肉关节损害2例(2.5%)、泌尿系统1例(1.3%)。结论小儿肺炎支原体易引起肺外脏器损害,对于常规治疗不理想及诊断不明确患儿,应注意支原体感染,尤其是以肺外脏器损害为首发症状者。

  6. 肺炎支原体体外诱导抗生素耐药及其机制%Resistance mechanisms to antibiotic in vitro in mycoplasma pneumoniae

    Institute of Scientific and Technical Information of China (English)

    史大伟; 辛德莉

    2010-01-01

    肺炎支原体(MP)是儿童和青少年社区获得性呼吸道感染的常见病原体,对大环内酯类、四环素类、氨基糖苷类、喹诺酮类等敏感,大环内酯类抗生素是治疗小儿MP感染的首选药物.然而,近年来在实验室诱导出对抗生素耐药的MP,并且体外分离到对大环内酯类抗生素耐药的菌株,结合位点的基因突变是目前的研究热点,也是引起MP耐药的主要机制.对MP耐药性的研究可为临床抗生素的合理选择和应用提供理论指导.%Mycoplasma pneumoniae(MP) is a common pathogen found in respiratory tract infections of children and is susceptible to macrolides, tetracycline, aminogly-cosides, and quinoloines . Of all these antibiotics, macrolides is the frist choice for children. However,in recent years, strains which are resistant to common drugs have been selected in vitro and isolated from patients Point mutation at antibitics target site of these 8trains is one of the molecular mechanisms.The study of MP resistance can provide theoretical guidance for rational choice of antibiotics and application.

  7. Immune responses elicited by Mycoplasma hyopneumoniae recombinant antigens and DNA constructs with potential for use in vaccination against porcine enzootic pneumonia.

    Science.gov (United States)

    Virginio, Veridiana Gomes; Gonchoroski, Taylor; Paes, Jéssica Andrade; Schuck, Desirée Cigaran; Zaha, Arnaldo; Ferreira, Henrique Bunselmeyer

    2014-10-07

    Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (PEP) and causes major economic losses to the pig industry worldwide. Commercially available vaccines provide only partial protection and are relatively expensive. In this study, we assessed the humoral and cellular immune responses to three recombinant antigens of M. hyopneumoniae. Immune responses to selected domains of the P46, HSP70 and MnuA antigens (P46102-253, HSP70212-601 and MnuA182-378), delivered as recombinant subunit or DNA vaccines, were evaluated in BALB/c mice. All purified recombinant antigens and two DNA vaccines, pcDNA3.1(+)/HSP70212-601 and pcDNA3.1(+)/MnuA182-378, elicited a strong humoral immune response, indicated by high IgG levels in the serum. The cellular immune response was assessed by detection of IFN-γ, IL-10 and IL-4 in splenocyte culture supernatants. The recombinant subunit and DNA vaccines induced Th1-polarized immune responses, as evidenced by increased levels of IFN-γ. All recombinant subunit vaccines and the pcDNA3.1(+)/MnuA182-378 vaccine also induced the secretion of IL-10, a Th2-type cytokine, in large quantities. The mixed Th1/Th2-type response may elicit an effective immune response against M. hyopneumoniae, suggesting that P46102-253, HSP70212-601 and MnuA182-378 are potential novel and promising targets for the development of vaccines against PEP.

  8. Evaluation of different nucleic acid amplification techniques for the detection of M. pneumoniae, C. pneumoniae and Legionella spp. in respiratory specimens from patients with community-acquired pneumonia

    NARCIS (Netherlands)

    Loens, K; Beck, T; Ursi, D; Overdijk, M; Sillekens, P; Goossens, H; Ieven, M; Niesters, Bert

    2008-01-01

    The number of pathogens involved in community-acquired pneumonia, with varying susceptibilities to antimicrobials, is numerous constituting an enormous challenge for diagnostic microbiology. Differentiation of infections due to Streptococcus pneumoniae and those due to Mycoplasma pneumoniae, Chlamyd

  9. Correlação entre sinais clínicos da conjuntivite felina e a detecção molecular de felid herpesvirus-1, feline calicivirus, chlamydophila felis e mycoplasma felis em gatos de abrigos no Rio de Janeiro

    Directory of Open Access Journals (Sweden)

    Natasha Baumworcel

    2017-05-01

    Full Text Available O objetivo deste estudo foi realizar diagnóstico molecular de agentes microbiológicos (FHV-1, FCV, Mycoplasma felis e Chlamydophila felis em gatos filhotes e associar a presença dos patógenos à gravidade dos sinais clínicos de conjuntivite. Foram coletadas um total de 108 amostras de suabe conjuntival de filhotes felinos assintomáticos (G1; n = 40 e sintomáticos (G2; n = 68. Animais do G2 foram categorizados de 1 (leve até 4 (grave, de acordo com o quadro clínico de conjuntivite. As 108 amostras foram submetidas à PCR e RT-PCR. O FHV-1 foi detectado em 57,4% das amostras, o FCV em 37%, o M. felis em 10,2% e o C. felis em 24,1%. Coinfecções, por sua vez, foram detectadas em 36,1%. No G1, 70% das amostras foram positivas para um ou mais patógenos. No G2, 85,3% apresentavam infecções (P = 0,03. No G1, monoinfecções por FHV-1 foram diagnosticadas em 52,5% das amostras, por FCV em 5%, por C. felis em 2,5%, e em 30% das amostras analisadas nenhum dos patógenos estudados foi encontrado. Coinfecções, por sua vez, estavam presentes em 72,5% das amostras. No G2, monoinfecções por FHV-1 foram encontradas em 45,6% das amostras, por FCV em 14,7 %, por M. felis em 3% e por C. felis também em 3%. Nenhum dos patógenos estudados foi encontrado em 14,7% das amostras analisadas. Coinfecções, responsáveis por 52% dos casos, foram categorizados como Grau 1 (29,4%, Grau 2 (20,6%, Grau 3 (30,9% e Grau 4 (19,1%. A presença de FHV-1 e FCV está igualmente distribuída entre as quatro categorias. Os sinais clínicos mais graves (graus 3 e 4 estão relacionados a coinfecções por C. felis e M. felis. Os agentes microbiológicos FHV-1, FCV, C. felis e M. felis foram encontrados em animais com conjuntivite. Coinfecções estão relacionadas aos casos mais graves. Por fim, concluiu-se que o diagnóstico molecular, além de detectar portadores assintomáticos, é um método rápido e acurado para o diagnóstico do patógeno causador da

  10. Clinical Analysis of Extra-Pulmonary Damage in Children with Mycoplasma Pneumoniae Infection in Yantian District%盐田区儿童肺炎支原体感染并肺外损害临床分析

    Institute of Scientific and Technical Information of China (English)

    唐红; 汪青; 张立福

    2011-01-01

    Objective To observe the clinical features of mycoplasma pneumonia in children lived in Yantian District, improve our awareness of mycoplasma pneumoniae infection, and reduce the rate of misdiagnosis and mistreatment of mycoplasma pneumonia. Methods The clinical data of 138 hospitalized children with mycoplasma pneumonia in our hospital from 2008 -2010 years were analyzed retrospectively. Results In all 138 patients, 33 cases were with damage to the digestive svstem:liver function showed elevated ALT, hepatomegaly or vomiting, loss of appetite and diarrhea; 11 patients with myocardial injury: palpitations,chest tightness, pale, shortness of breath and increased performance of specific myocardial enzymes, or ECG abnormalities ;3 cases with impaired renal function;3 cases with leukemoid reaction by blood test; 12 patients with lower blood leukocytes;2 cases with the history of convulsion. Conclusion Mycoplasma pneumoniae infection,can not only lead to the upper respiratory tract infection, bronchitis,bronchial pneumonia and other respiratory diseases, but also lead to the extrapulmonary multi-system damage,mainly in digestive system and cardiovascular system, which can induce serious complications and even life-threatening. Therefore, clinicians should pay more attentions on the careful observation of condition changes, laboratory tests of the relevant system,complications and timely diagnosis and treatment.%目的 通过观察盐田区儿童支原体肺炎的临床特征,提高对肺炎支原体感染的认识,减少误诊误治.方法 回顾性分析深圳市盐田区人民医院2008±2010年儿科住院138例支原体肺炎患儿的临床资料.结果 138例患儿中33例合并消化系统损害:表现为肝功能ALT升高或合并肝肿大,或呕吐,食欲不振,腹泻等.11例合并心肌损害:表现为心悸,胸闷,面色苍白,气促等,实验室检查示特异性心肌酶谱升高,或心电图异常.3例合并肾功能损害.3例血常规

  11. Pneumonia

    Science.gov (United States)

    ... better than treating it. Vaccines are available to prevent pneumococcal pneumonia and the flu. Other preventive measures include washing your hands frequently and not smoking. NIH: National Heart, Lung, and Blood Institute

  12. Anticuerpos contra Chlamydophilaen pacientes con infarto agudo del miocardio y riesgo coronario, y su relación con la muerte Antibodies against Chlamydophila in patients with acute myocardial infarction and coronary risk and their association with mortality

    Directory of Open Access Journals (Sweden)

    Guadalupe García-Elorriaga

    2005-06-01

    Full Text Available OBJETIVO: Determinar si los anticuerpos contra Chlamydophila pneumoniae en pacientes con infarto agudo del miocardio y factores de riesgo coronario se asocian con la muerte. MATERIAL Y MÉTODOS: Se hizo un estudio observacional, prospectivo, transversal y comparativo. Se incluyeron en el estudio 100 sujetos que, entre 1999 y 2000, estuvieron hospitalizados en la Unidad Coronaria del Hospital de Especialidades del Centro Médico La Raza, del Instituto Mexicano del Seguro Social. Se trataba de una muestra constituida por pacientes de ambos sexos, mayores de 18 años, con infarto agudo del miocardio y riesgo coronario. Mediante microinmunofluorescencia indirecta se identificaron anticuerpos contra Chlamydophila pneumoniae, Chlamydophila psitacii y Chlamydia trachomatis. De entre los 100 sujetos, se eligieron al azar 33, a quienes se les determinaron anticuerpos contra Chlamydophila, no sólo durante su estancia en el hospital, sino también al salir de éste y a los tres meses de haber sufrido el infarto agudo del miocardio. Se calcularon las medias y las desviaciones geométricas estándares para los títulos de anticuerpos contra Chlamydophila, y se determinó la razón de momios y el intervalo de confianza al 95% entre los factores de riesgo coronario y la muerte. RESULTADOS: Setenta por ciento de los pacientes de la muestra inicial presentaron anticuerpos contra Chlamydophila pneumoniae; no se identificaron anticuerpos contra Chlamydophila psitacii y Chlamydia trachomatis. No se observó una fuerza de asociación estadísticamente significativa con la muerte en pacientes con infarto agudo del miocardio y factores de riesgo coronario. De los 33 individuos de la submuestra, 25 presentaron anticuerpos contra Chlamydophila pneumoniae, y en 83% de estos últimos casos, se registró un descenso de dichos anticuerpos a los tres meses de haberse presentado el infarto agudo del miocardio. CONCLUSIONES: A pesar de que en pacientes con infarto agudo del

  13. The Effect of the Treatment for Children’s Mycoplasma Pneumonia with Azithromycin and Phentolamine%阿奇霉素联合酚妥拉明治疗小儿支原体肺炎临床观察

    Institute of Scientific and Technical Information of China (English)

    洪福秀; 曹青山

    2015-01-01

    目的:重点研究和探讨阿奇霉素联合酚妥拉明治疗小儿支原体肺炎的临床疗效及安全性。方法:选取2011年12月-2014年1月本院接收诊治的120例小儿支原体性肺炎患儿的临床资料进行回顾性对比分析。随机数字表法分为两组,对照组60例应用阿奇霉素单药进行治疗,观察组60例应用阿奇霉素联合酚妥拉明进行治疗,比较两组患儿的疗效。结果:观察组治疗总有效率为93.3%,对照组为81.7%,两组比较差异有统计学意义(P<0.05);两组症状、体征消失时间比较差异均有统计学意义(P<0.05);且两组均未出现明显副作用。结论:阿奇霉素联合酚妥拉明治疗支原体性肺炎患儿临床疗效显著,且具有较好的安全性,已经成为治疗小儿支原体性肺炎的可靠方法,对促进治疗小儿支原体性肺炎的发展具有重要的价值,值得临床推广使用。%Objective:To study the effect and the security of the treatment on the children’s mycoplasma pneumonia with Azithromycin and Phentolamine. Method:120 children with mycoplasma pneumonia in our hospital from December 2011 to January 2014 were selected and randomly divided into two groups. The control group(n=60) were treated with Azithromycin only,the experimental group(n=60) were treated with Azithromycin and Phentolamine. The treatment effect of two groups was compared. Result:The effective rate in the experimental group was 93.3%,the control group was 81.7%,the two groups had statistical significance (P<0.05). The signs and symptoms disappeared time between the two groups had statistical significance(P<0.05). There were not significant side effects in the two groups. Conclusion:The curative effect treating children’s mycoplasma pneumonia with Azithromycin and Phentolamine is distinct,what’s more,its security is very good,so it has been a commonly way to treat children’s mycoplasma pneumonia,it is valuable for treating

  14. 成人支原体肺炎并发溶血性贫血一例并文献复习%Mycoplasma pneumonia associated with hemolytic anemia: case report and literature review

    Institute of Scientific and Technical Information of China (English)

    韩翔; 贺蓓; 王飞

    2011-01-01

    目的 提高临床医生对成人支原体肺炎并发溶血性贫血的认识.方法 报道1例成人支原体肺炎并发溶血性贫血病例,以mycoplasma pneumonia、hemolytic anemia、adult及case report为检索词,检索时限为1967年1月至2011年3月,通过PubMed检索系统进行检索;同时以“肺炎,支原体,溶血性贫血,成人”为检索词,检索时限为1978年1月至2011年3月,通过万方数据库对中文文献进行检索,共检索出相关临床资料完整的病例报告9篇.结合国内外文献报道的9例同类病例的临床资料进行分析.结果 患者女,29岁,因“发热伴咳嗽、咳痰15 d,血红蛋白下降2d”入院.X线胸片示左下肺渗出性病变,发病第14天外周血血红蛋白下降,最低至83 g/L,网织红细胞为0.04,同时室温下抽出的静脉血在试管中凝集,血清支原体抗体IgM阳性,冷凝集素滴度为1∶256.经抗感染治疗2周后血红蛋白含量恢复正常,肺部病变消失,诊断为支原体肺炎并发溶血性贫血.复习国内外文献报道的9例临床资料完整的成年病例,10例中男6例,女4例,年龄29 ~60岁,8例在发病第8~18天出现溶血性贫血,10例均出现血冷凝集素滴度增高(其中7例>1∶1024),2例发生血管内栓塞.10例中9例痊愈,1例死亡.结论 支原体肺炎并发溶血性贫血多发生在发病第8~18天,与冷凝集素滴度增高相关,部分病例同时出现血管内栓塞.多数病例预后良好,少数重症病例预后不良.%Objective To improve the understanding of hemolytic anemia secondary to Mycoplasma pneumonia.Methods One case of hemolytic anemia secondary to Mycoplasma pneumonia in an adult was reported.With “Mycoplasma pneumonia,hemolytic anemia,adult,case report” as the search terms,and the search time ranged from 1967.1 to 2011.3 for PubMed and 1978.1 to 2011.3 for Wanfangdata,9 cases of similar clinical cases in the literatures were reviewed.Results A 29 year-old female patient was

  15. Application of comprehensive nursing intervention in the treatment of children patients with mycoplasma pneumoniae pneumonia%综合护理干预在支原体性肺炎患儿中的应用

    Institute of Scientific and Technical Information of China (English)

    汤敏

    2012-01-01

    Objective: To investigate the methods and clinical effect of comprehensive nursing intervention in the treatment of children patients with mycoplasma pneumoniae pneumonia( MPP ). Method: 112 children patients with MPP were randomly divided into an intervention group and a control group ( 56 cases in each group ). The patients in the control group were given conventional nursing care and the comprehensive nursing intervention was implemented in the intervention group based on the conventional nursing care, including psychological care,aerosol inhalation and sputum elimination by percussion vibration on the back of patients. The disappearance time of symptoms and signs and clinical effect were compared between the two groups. Results:The disappearance time of fever,cough and pulmonary rales was earlier in the intervention group than that in the control group ( P < 0. 05 ); the clinical effect was better in the intervention group than the control group ( P<0.05 ). Conclusion: The comprehensive nursing intervention can improve the treatment outcome and is conducive to the rehabilitation of patients with MPP.%目的:探讨综合护理干预在支原体性肺炎(MPP)患儿中的应用方法及临床效果.方法:将112例MPP患儿随机分为干预组和对照组各56例,对照组给予常规护理,干预组在此基础上给予心理护理、雾化吸入及胸背部叩击振动排痰等综合护理干预.比较两组症状、体征消失时间及临床疗效.结果:干预组发热、咳嗽和肺部罗音消失时间较对照组早(P<0.05),临床疗效优于对照组(P<0.05).结论:综合护理干预可提高MPP患儿的治疗效果,有利于康复.

  16. Development of an endpoint genotyping assay to detect the Mycoplasma pneumoniae 23S rRNA gene and distinguish the existence of macrolide resistance-associated mutations at position 2063.

    Science.gov (United States)

    Suzuki, Yu; Seto, Junji; Shimotai, Yoshitaka; Ikeda, Tatsuya; Yahagi, Kazue; Mizuta, Katsumi; Matsuzaki, Yoko; Hongo, Seiji

    2016-12-01

    The prevalence of macrolide-resistant Mycoplasma pneumoniae harboring a mutation in the 23S rRNA gene is increasing, and rapid detection assays are needed for clinical management. We developed an endpoint genotyping assay to detect the M. pneumoniae 23S rRNA gene and determine the existence of macrolide resistance-associated mutations at position 2063 (A2063G, A2063T and A2063C mutations). This A2063B genotyping assay detected more than 50 copies/reaction of the M. pneumoniae gene in every nucleotide mutation at position 2063. Of 42 clinical specimens, 3 were positive without mutation, 6 were positive with the A2063G mutation, and 33 were negative. The results were confirmed using nested PCR with the sequencing of the M. pneumoniae 23S rRNA gene, and a high sensitivity (90%), specificity (100%), and coincidence ratio (kappa coefficient=0.93) were obtained. Therefore, the A2063B genotyping assay is useful for the rapid discrimination of macrolide resistance mutations at position 2063.

  17. Clinical characteristics of Q fever and etiology of community-acquired pneumonia in a tropical region of southern Taiwan: a prospective observational study.

    Directory of Open Access Journals (Sweden)

    Chung-Hsu Lai

    Full Text Available The clinical characteristics of Q fever are poorly identified in the tropics. Fever with pneumonia or hepatitis are the dominant presentations of acute Q fever, which exhibits geographic variability. In southern Taiwan, which is located in a tropical region, the role of Q fever in community-acquired pneumonia (CAP has never been investigated.During the study period, May 2012 to April 2013, 166 cases of adult CAP and 15 cases of acute Q fever were prospectively investigated. Cultures of clinical specimens, urine antigen tests for Streptococcus pneumoniae and Legionella pneumophila, and paired serologic assessments for Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Q fever (Coxiella burnetii were used for identifying pathogens associated with CAP. From April 2004 to April 2013 (the pre-study period, 122 cases of acute Q fever were also included retrospectively for analysis. The geographic distribution of Q fever and CAP cases was similar. Q fever cases were identified in warmer seasons and younger ages than CAP. Based on multivariate analysis, male gender, chills, thrombocytopenia, and elevated liver enzymes were independent characteristics associated with Q fever. In patients with Q fever, 95% and 13.5% of cases presented with hepatitis and pneumonia, respectively. Twelve (7.2% cases of CAP were seropositive for C. burnetii antibodies, but none of them had acute Q fever. Among CAP cases, 22.9% had a CURB-65 score ≧2, and 45.8% had identifiable pathogens. Haemophilus parainfluenzae (14.5%, S. pneumoniae (6.6%, Pseudomonas aeruginosa (4.8%, and Klebsiella pneumoniae (3.0% were the most common pathogens identified by cultures or urine antigen tests. Moreover, M. pneumoniae, C. pneumoniae, and co-infection with 2 pathogens accounted for 9.0%, 7.8%, and 1.8%, respectively.In southern Taiwan, Q fever is an endemic disease with hepatitis as the major presentation and is not a common etiology of CAP.

  18. 肺炎支原体耐药机制研究进展%Research progress in mechanism of drug resistance of Mycoplasma pneumoniae

    Institute of Scientific and Technical Information of China (English)

    过毅; 葛小丽; 吴莹莹; 周晓飞

    2016-01-01

    Macrolides antibiotics are the first choice for children infected by Mycoplasma pneumoniae(MP) which is a common pathogen of community acquired infections ,as well as hospital acquired infections .Drug resistance of MP is quite severe in China ,it has an important clinical significance to investigate the drug resistance mechanism of MP .This review summarized the current status of drug resistance of MP ,and introduced the research progress of drug resistance mechanism in recent years .The key mechanism of drug resistance was thought to be mutations of genes located in domain V of 23SrRNA and protein L4 and L22 .Relevant researches also focused on new mech‐anisms .Studies of drug resistance mechanisms will provide theoretical basis for the development of antibiotics , vaccines and targeted drugs .Clinicians should pay attention to the severe drug resistance of MPand use antibiotics properly .Meanwhile ,the monitoring and management of MP drug resistance should be improved .%肺炎支原体(M P )是社区获得性及医院获得性感染常见病原体,大环内酯类抗菌药物为治疗儿童M P感染首选药物,但国内大环内酯类耐药情况严重,探讨MP耐药机制有重要的临床意义;简述了目前MP耐药现状,介绍了近年来耐药机制的研究进展,指出了耐药机制主要为23SrRNA中V区基因及L4、L22蛋白突变,是否存在新的耐药机制仍在研究中;耐药机制的研究将为抗菌药物改造、疫苗、靶向药物的研发提供理论基础,提出了临床医师应重视MP高耐药性的严峻形势,规范使用抗菌药物,同时应完善MP耐药性的监测和管理。

  19. 盐酸氨溴索辅佐阿奇霉素治疗儿童肺炎支原体肺炎疗效评价%Curative effect evaluation of ambroxol hydrochloride assisting azithromycin in the treatment of children with myco-plasma pneumoniae pneumonia

    Institute of Scientific and Technical Information of China (English)

    孙庆霞

    2016-01-01

    目的:探讨盐酸氨溴索联合阿奇霉素治疗儿童肺炎支原体肺炎的临床疗效。方法:收治肺炎支原体肺炎患儿100例,随机分为两组,每组50例。对照组给予阿奇霉素治疗及对症支持治疗,试验组在对照组基础上给予盐酸氨溴索治疗,比较两组临床疗效。结果:试验组总有效率98.00%,对照组总有效率60.00%。试验组疗效优于对照组,差异具有统计学意义(P<0.05)。结论:盐酸氨溴索联合阿奇霉素治疗儿童肺炎支原体肺炎的临床疗效显著,能促进患儿肺功能的恢复。%Objective:To investigate the clinical curative effect of ambroxol hydrochloride combined with azithromycin in the treatment of children with mycoplasma pneumoniae pneumonia.Methods:100 cases of children with mycoplasma pneumoniae pneumonia were divided into two groups randomly,with 50 cases in each group.Patients in the control group were given azithromycin and symptomatic and supportive treatment.Patients in the experiment group were given ambroxol hydrochloride on the basis of the control group.The clinical curative effect of two groups was compared.Results:The total effective rate of the experiment group was 98.00%,and the total effective rate of the control group was 60.00%.The curative effect of the experiment group was better than that of the control group,and the difference was statistically significant(P<0.05).Conclusion:Ambroxol hydrochloride combined with azithromycin in the treatment of children with mycoplasma pneumoniae pneumonia had significant clinical curative effect and could promote the recovery of pulmonary function in children.

  20. High times microscopic analyzer in the diagnosis of mycoplasma pneumoniae infection in children%超高倍显微分析仪在儿童肺炎支原体感染诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    石龙姣

    2015-01-01

    目的 探讨超高倍显微分析仪检测法在儿童肺炎支原体( MP)感染中的诊断价值及应用. 方法 对196例疑似 MP感染的呼吸道感染患儿,用超高倍显微分析仪对其咽分泌物直接镜检观察,同时于发病7d后,测血清MP-IgM,对二者结果进行比较. 结果 超高倍显微分析仪和血清MP-IgM法检出肺炎支原体阳性率分别为41.3%和39.3%,二种检测方法结果比较,其阳性率差异无统计学意义(P>0.05). 结论 超高倍显微分析仪直视活体检测法简单易行、准确快捷,是一种儿童肺炎支原体感染早期检测很好的新方法.%Objective To study the high times microscopic analyzer test mycoplasma pneumoniae ( MP) infection in children and the diagnostic value of application . Methods 196 cases of suspected MP infection in children with respiratory tract infection ,with high times microanalyzer pharynx secretion to its direct microscopy observa-tion,at the same time at 7d after onset,measuring serum MP IgM,comparing with the result of the two.Results High times microanalyzer and serum MP IgM method de-tected mycoplasma pneumoniae positive rate were41.3%and39.3%respectively,comparing two kinds of detection method results,there was no statistically significant difference its positive rate(P>0.05).Conclusion The high times microscopic instrument direct living method is simple ,accurate and fast,is a children's pneumonia my-coplasma infection,a new method of early detection of good.

  1. The Study of Children Patients With Mycoplasma Pneumonia Infection With Clinical Testing Analysis%临床检验分析对小儿肺炎支原体感染的研究

    Institute of Scientific and Technical Information of China (English)

    赵文凤; 周文斌

    2015-01-01

    Objective Clinical testing methods for children patients with mycoplasma pneumonia infection are to be studied.MethodsReviewed and analyzed clinical testing results selected from 41 children patients with mycoplasma pneumonia infection who were treated in hospital from April 2013 to April 2015.Results There were 33 normal cases and 8 abnormal cases tested by urine routine examination respectively,11 cases of leucocytes count 10.0×109/L,7 cases of platelets count 20 mm/h,21 cases’ CRP >10 mg/L;11 cases of bacteria culture results were positive.Conclusion For treatment children patients with mycoplasma pneumonia infection,it is suggested to apply a comprehensive analysis to clinical testing,including urine routine test,blood routine test,vigor analysis,passive agglutination test and bacterial culture of sputum,etc,in order to improve diagnosis accuracy and prevent from misdiagnosis.%目的:深入研究小儿肺炎支原体感染的临床检验方法。方法选取于2013年4月~2015年4月期间在我院接受治疗的41例小儿肺炎支原体感染患儿的临床检验结果进行回顾性分析。结果尿常规检查正常的有33例,异常8例;11例白细胞计数<4.0×109/L,8例白细胞计数在(4.0~10.0)×109/L,22例白细胞计数>10.0×109/L;7例血小板<100×109/L,34例血小板≥100×109/L;表现为MP-IgM阳性的有27例;20例血沉>20 mm/h,21例CRP>10 mg/L;11例细菌培养结果显示为阳性。结论在对小儿肺炎支原体感染进行诊断时,要通过尿常规、血常规、血气分析、被动凝集试验、对痰液进行细菌培养等临床检验进行综合分析,提高确诊率,避免出现漏诊误诊的情况。

  2. Clinical manifestations and diagnosis and treatment on 200 cases of infantile pneumonia mycoplasma infection%小儿肺炎支原体感染200例临床表现及诊治

    Institute of Scientific and Technical Information of China (English)

    张笑云

    2014-01-01

    Objective to study the clinical symptoms and manifestations of pneumoniae mycoplasma infection in children. Methods the data of 200 cases of children with mycoplasma pneumoniae infection were retrospectively analyzed including symptoms, signs and auxiliary examination.Results on the early onset of disease, 200 cases often suffered from symptoms such as fever, cough and headache, at the same time, with a certain degree of neurological symptoms. the majority of cases showed white blood cells increasing and more than half cases showed abnormal performance by radiographic examination of the chest. Conclusion Mycoplasma pneumoniae infection has serious injuries on children, with faster development and poor prognosis. Because of the lack of speciifcity in clinical feature, it is dififcult for clinical diagnosis. The physicians should improve the ability on disease diagnosis and pay more attention to this.%目的:分析探讨小儿肺炎支原体感染的发病特征与临床表现。方法回顾性分析我院200例小儿肺炎支原体感染患儿的发病症状、体征及辅助检查等资料。结果200例患儿均于起病初期显现发热、咳嗽、头痛症状,同时伴有一定程度的神经系统症状。多数出现全血白细胞增高,胸部放射线检查半数以上有异常。结论小儿肺炎支原体感染对患儿损伤重,发展比较快,多预后较差,由于缺乏特异性临床特征,为临床诊断制造困难,要求医师提高诊断水平,充分给予重视。

  3. Improved sensitivity of PCR for Chlamydophila using pmp genes.

    Science.gov (United States)

    Laroucau, K; Souriau, A; Rodolakis, A

    2001-09-20

    Primers targeting the conserved pmp gene family of Chlamydophila abortus were evaluated for their ability to improve the polymerase chain reaction (PCR) sensitivity. In purified DNA, specific pmp primers (named CpsiA and CpsiB) allowed at least a 10-fold increase of the PCR sensitivity compared to the specific ompA primers for C. abortus, but also for C. psittaci and C. caviae strains. No amplification was observed on C. felis, C. pecorum, C. pneumoniae and Chlamydia trachomatis strains. Tested on contaminated specimens such as genital swabs, the PCR sensitivity observed with CpsiA/CpsiB was also better than with the ompA primers. This study demonstrated that these specific pmp primers could serve as valuable, sensitive and common tools for a specific Chlamydophila diagnosis in ruminant, avian and human diseases. Digestion by AluI of the CpsiA/CpsiB fragments allowed a specific discrimination of the strains in function of their hosts and/or their serotypes.

  4. 肺炎嗜衣原体热休克蛋白10促炎症作用及其机制的初步研究%Role and preliminary mechanism of heat shock protein 10 of Chlamydophila pneumoniae in inducing inflammatory reaction

    Institute of Scientific and Technical Information of China (English)

    周洲; 陈丽丽; 刘良专; 李忠玉; 游晓星; 陈曦; 吴移谋

    2013-01-01

    目的 探讨肺炎嗜衣原体(Chlamydophila pne umoniae,Cpn)热休克蛋白(heat shock protein,HSP)10(CHSP10)诱导人单核细胞分泌炎症因子的作用及Toll样受体(Toll-like receptor,TLR)2、TLR4与此作用的相关性.方法 以去内毒素活性的不同质量浓度CHSP10(0.5、1、5、10、20、30 μg/ml)刺激THP-1细胞0、6、12、24、36、48、60 h,检测蛋白未处理组、加热处理组、去蛋白处理组中IL-1β及IL-6水平;用CHSP10刺激C3H系野生型(C3H/HeN)和TLR4缺陷型(C3H/HeJ)小鼠腹腔巨噬细胞,分别检测II-1β及IL-6水平;用CHSP10刺激被抗TLR2/TLR4抗体处理的THP-1细胞,检测IL-1β、IL-6的变化.结果 CHSP10可诱导THP-1细胞产生炎症因子IL-1β、IL-6;CHSP10诱生C3H系野生型小鼠细胞分泌的炎症因子明显高于TLR4缺陷型小鼠细胞;用TLR2和/或TLR4抗体封闭后,CHSP10诱生的IL-1β、IL-6不同程度减少.结论 CHSP10可能作为炎症相关蛋白参与了Cpn对宿主细胞的致炎作用;并且TLR2及TLR4在该炎症刺激信号的传递过程中发挥一定的作用.%To investigate the role and mechanism of heat shock protein 10 (HSP10) of Chlamydophila pneumoniae in inducing inflammatory reaction. Purified native recombinant HSP10 from C. pneumoniae (CHSP10) were inactivated for cleaning contaminated endotoxin and were used to stimulate THP -1 with different concentrations for different time, then the cytokines levels were measured by using human enzyme -linked immunosorbent assay kit. Peritoneal macrophages from TLR4-deficient mice and wide-type mice were stimulated with endotoxin -free proteins respectively, and the cytokines levels were measured. Furthermore, blocking experiments with neutralizing anti-human TLR2 or/and TLR4 MAb were carried out and ELISA was used to detect the concentration of cytokines. Result showed that HSP10 was able to induce IL—1β and IL-6 secretion in THP-1. Wild-type macrophages from C3H/HeN secreted higher IL—1β and IL-6 level

  5. Analysis of 11 cases of mycoplasma pneumoniae infection combined with Kawasaki disease%川崎病合并肺炎支原体肺炎11例分析

    Institute of Scientific and Technical Information of China (English)

    朱巧; 过建春

    2013-01-01

    Objective To study the clinical characteristics of pediatric Kawasaki disease complicating mycoplasma pneumoniae pneumonia.Methods Retrospective analysis was conducted on 11 children who had been diagnosed with Kawasaki disease with Mycoplasma pneumoniae pneumonia.Results The 11 cases presented with varying degrees of fever,conjunctival congestion,skin rashes,lymphadenectasis,distal extremities lesions,heart and lung lesions.8 of them were standartly treated with azithromycin,while 3 of them were treatad with azithromycin and erythromycin.2 patients with pleural effusion complicated by lobar pneumonia consolidation were treated with gamma globulin combined aspirin.All of the 11 patients were healed.Conclusion Infections are common at the diagonosis of KD.Reasonable examination and antibiotics is useful to cure KD with MPP.%目的 分析川崎病(KD)合并肺炎支原体肺炎(MPP)临床特点,以提高对KD合并MPP的认识和诊治水平.方法 对临床诊断为川崎病的11例患儿的临床资料进行回顾性分析.结果 11例患儿出现不同程度的发热、眼结膜充血、皮疹、淋巴结肿大、四肢末梢病变、心脏及肺部病变等情况;8例患儿采用阿奇霉素规范治疗,3例患儿采用阿奇霉素并红霉素治疗,其中2例大叶性肺炎实变合并胸腔积液患儿应用丙种球蛋白联合阿司匹林治疗,均痊愈.结论 肺炎支原体肺炎导致的免疫紊乱可能与川崎病的发生有关,抗感染治疗可改善预后.

  6. Mycoplasma alkalescens demonstrated in bronchoalveolar lavage of cattle in Denmark

    DEFF Research Database (Denmark)

    Kokotovic, Branko; Friis, Niels F.; Ahrens, Peter

    2007-01-01

    Mycoplasma alkalescens is an arginine-metabolizing mycoplasma, which has been found in association with mastitis and arthritis in cattle. Routine bacteriological examination of 17 bronchoalveolar lavage samples from calves with pneumonia in a single herd in Denmark, identified M. alkalescens...

  7. Participação do Mycoplasma pneumoniae na etiologia de infecções respiratórias agudas em Ribeirão Preto, São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Alcyone A. Machado

    1991-03-01

    Full Text Available Para avaliar a participação etiológica do Mycoplasma pneumoniae em infecções respiratórias agudas, o isolamento dessa bactéria foi tentado em secreções do aparelho respiratório de 64 pacientes (média 24 anos com quadro respiratório aguda Foi realizada, também, a pesquisa de anticorpos específicos anti-M. pneumoniae através da reação de fixação do complemento (FC e da reação de contra-imunoeletroforese (CIE. O M. pneumoniae não foi isolado. O diagnóstico presuntivo de infecção pelo M. pneumoniae foi feito pela FC em 3,1% (2/64 e pela CIE em 1,6% (1/64 dos pacientes. Paralelamente, em 200 indivíduos sadios, os mesmos testes sorológicos foram realizados, sendo o índice de positividade de 4% (8/200 pela CIE e de 1% (2/200 pela FC. Apesar das aiscrepâncias observadas entre os dois métodos sorológicos, a FC parece ser indicada para diagnóstico da infecção, sendo a CIE recomendada nas avaliações soroepidemiológicas. Com base nos dados do nosso estudo, a prevalência das infecções respiratórias pelo M. pneumoniae parece ser baixa em nosso meio.

  8. Therapeutic Chlamydophila abortus and C. pecorum vaccination transiently reduces bovine mastitis associated with Chlamydophila infection.

    Science.gov (United States)

    Biesenkamp-Uhe, Carolin; Li, Yihang; Hehnen, Hans-Robert; Sachse, Konrad; Kaltenboeck, Bernhard

    2007-02-01

    Infections with Chlamydophila abortus and C. pecorum are highly prevalent in cattle and have been associated with bovine mastitis. A prospective cohort study was conducted with a herd of 140 Holstein dairy cows to investigate the influence of Chlamydophila infection on subclinical inflammation of the bovine mammary gland as characterized by somatic cell numbers in milk. PCR detection of C. abortus and low serum antibody levels against Chlamydophila spp. were significantly associated with subclinical mastitis. To examine the effect of the infection by response modification, immune perturbation was done by two subcutaneous administrations of an experimental vaccine preparation of inactivated C. abortus and C. pecorum elementary bodies. Vaccination against Chlamydophila highly significantly decreased milk somatic cell numbers, thus reducing bovine mastitis, and increased antibody levels against Chlamydophila but did not eliminate shedding of C. abortus in milk as detected by PCR. The protective effect peaked at 11 weeks after vaccination and lasted for a total of 14 weeks. Vaccination with the Chlamydophila vaccine, a mock vaccine, or a combination vaccine against bovine viral diseases highly significantly increased C. abortus shedding in milk for 1 week, presumably mediated by the vaccine adjuvant. In summary, this study shows an etiological involvement of the widespread Chlamydophila infections in bovine mastitis, a herd disease of critical importance for the dairy industry. Furthermore, this investigation shows the potential for temporary improvement of chlamydial disease by therapeutic vaccination. Chlamydophila vaccination of cattle might serve as a testing ground for vaccines against human chlamydial infections.

  9. Clinical research on mycoplasma pneumoniae complicated with digestive system damage in children%小儿肺炎支原体感染并发消化系统损害临床分析

    Institute of Scientific and Technical Information of China (English)

    韦秋玲

    2016-01-01

    目的:总结小儿肺炎支原体感染并发消化系统损害的临床表现。方法对271例肺炎支原体感染患儿的临床资料进行回顾性分析。结果271例肺炎支原体感染患儿中肺外损害检出率为52.77%(143/271),其中消化系统损害检出率为34.69%(94/271),占肺外损害的65.73%(94/143)。男性并发消化系统损害的检出率(33.80%)与女性(35.66%)之间差异无统计学意义(χ2=0.103,P=0.748)。婴幼儿组消化系统损害检出率(47.27%)高于儿童组(31.48%),差异有统计学意义(χ2=4.825,P=0.028)。婴幼儿组的临床表现以纳差、恶心、呕吐症状为主,儿童组以腹胀、腹痛症状为主。儿童组的血清转氨酶、血总胆红素、血淀粉酶、尿淀粉酶、腹部超声、腹部X线及腹部CT等异常检出率高于婴幼儿组,差异有统计学意义( P<0.05)。结论小儿肺炎支原体感染常并发消化系统损害,需积极预防和有效干预。%Objective To explore the clinical manifestations in children with mycoplasma pneumoniae com -plicated with the digestive system damage .Methods The clinical data of 271 children with mycoplasma pneumoniae were retrospectively studied .Results The rate of extrapulmonary organ damages in 271 children with mycoplasma pneumoniae was 52.77%(143/271), among which, the rate of the digestive system damage organ was 34.69%(94/271), accounting for 65.73%(94/143) of the extrapulmonary organ damages .There were no significant differences in concurrent digestive system organ damages between the male children ( 33.80%) and the female children (35.66%)(χ2 =0.103, P=0.748).The rate of digestive system organ damages of the infants (47.27%) was high-er than that of the children subgroup (31.48%), significantly(χ2 =4.825, P=0.028).The clinical manifestations of the infant subgroup were characterized with anorexia , nausea, and

  10. Research of relevance between damage of airway mucosa and the prognosis of patients with or without pro- tracted mycoplasma pneumoniae pneumonia%气道黏膜损害程度与迁延和非迁延性支原体肺炎的预后相关性研究

    Institute of Scientific and Technical Information of China (English)

    黄水东; 冯晓红

    2016-01-01

    目的 探讨气道黏膜损害程度与迁延和非迁延性支原体肺炎的预后相关性.方法 选取我院收治的124例支原体肺炎患者,按支原体肺炎分类标准及预后分为非迁延组和迁延组.记录并比较两组临床表现、气道黏膜损害程度及预后等情况.结果 124例支原体肺炎患者中28例演变为迁延性支原体肺炎;两组患者急性期临床症状无显著差异(P>0.05);支气管镜检查,两组均存在气道黏膜滤泡样增生、粗糙、水肿、纵行皱褶及黏液分泌增多等表现,其中管腔狭窄、肉芽组织增生及黏膜溃疡等仅存在于迁延组,迁延组滤泡样增生及黏液分泌显著增高(P0. 05). Through tracheobronchoscopy, both groups had follicular hyperplasia of airway mucosa, edema, rough, longitudinal folds and mucus secretion increased. The stenosis of airway, granulation tissue hyperplasia and mucosal ulcer existed only in the persistent group, and follicular hyperplasia and mucus secretion increased in the persistent group significantly(P<0. 05). Conclusion The dam-age of airway mucosa is related with the persistent course of patients with mycoplasma pneumoniae pneumonia, and follicular hyperplasia and mucus secretion increase are the causes of persistent mycoplasma pneumonia. The stenosis of airway, granulation tissue hyperplasia and mucosal ulcer mucosa damage suggest a prolonged course of patients with mycoplasma pneumoniae pneumonia.

  11. High Prevalence of Natural Chlamydophila Species Infection in Calves

    OpenAIRE

    Jee, JunBae; DeGraves, Fred J.; Kim, TeaYoun; Kaltenboeck, Bernhard

    2004-01-01

    We investigated the acquisition and prevalence of Chlamydophila sp. infection in calves. Specimens were collected at weekly intervals from birth to week 12 postpartum from 40 female Holstein calf-dam pairs in a dairy herd. Real-time PCR detected, quantified, and differentiated Chlamydophila 23S rRNA gene DNA from vaginal cytobrush swabs and milk samples. Chemiluminescence enzyme-linked immunosorbent assay with lysed Chlamydophila abortus or Chlamydophila pecorum elementary body antigens quant...

  12. 呼吸道感染患者肺炎支原体抗体检测结果分析%The analyses of Mycoplasma pneumoniae antibody test results in patients with respiratory tract infection

    Institute of Scientific and Technical Information of China (English)

    冯志敏; 程伟; 周祖发; 袁桂转

    2011-01-01

    目的 研究肺炎支原体(MP)感染发病率与患者年龄、性别和季节的关系.方法 用被动凝集法检测呼吸道感染患者血清中肺炎支原体抗体(MP-Ab),并对2010年患者MP-Ab检测结果进行分析.结果 5年检测结果阳性率为30.10%;男、女性患者阳性率分别为30.74%、36.12%,差异有统计学意义(P1:640的患者占10.18%.结论 MP感染逐年增加,3~14岁儿童为高危人群,女性感染机会高于男性,全年均可发病;大多患者预后良好.%Objective To investigate the relationship between the infection rate of Mycoplasma pneumoniae (MP) and age, sex and season. Methods Passive agglutination assay was used to detect Mycoplasma pneumoniae antibodies (MP-Ab) in the serum of patients with respiratory tract infection, and MP-Ab test results in 2010 were analyzed. Results The positive rates of 5 year test results were 30. 10% ;among the results, the positive rates of male and female patients were respectively 30. 74% and 36. 12% ,the difference was statistically significant ( P 1:640 accounted for 10. 18% of the patients. Conclusion MP infection is increasing year by year, children aged 3 to 14 has become the highrisk groups. Women are more susceptible to MP than the men and the chances of infection are throughout the year, hut the most of the patients have a good prognosis.

  13. Treatment of ultrashort wave therapy and cupping therapy on mycoplasma pneumonia%经络拔罐超短波治疗支原体肺炎83例临床观察

    Institute of Scientific and Technical Information of China (English)

    梁建军; 戈平

    2011-01-01

    Objective To study the effect of treatment of ultra-short wave therapy and cupping therapy on myco-plasma pneumonia. Method The treatment group (83 cases) was treated with ultrashort wave therapy and cupping therapy and the control group (41 cases) was treated with ultrashort wave therapy. Results The effective rate of treatment group and control group were 94% (78/83) and 83% (34/41) ( χ2 =8. 82, P<0. 01). Conclusion It is effective to treat mycoplasma pneumonia with ultrashort wave therapy and cupping therapy.%目的 现察经络拔罐超短波治疗小儿支原体肺炎的临床疗效.方法 治疗组83例,采用经络拔罐肺部超短波治疗,对照组41例单用肺部超短波治疗,两组均以5天为1个疗程,现察临床治疗效果.结果 治疗组痊愈54例,好转24例,治愈率65.1%,有效率93.98%;对照组痊愈21例,好转13例,治愈率51.3%,有效率82.93%两组有效率比较,差异有显著性(P<0.01).结论 采用经络拔罐和超短波治疗小儿支原体肺炎,消炎作用明显、,疗程短、有效率高,是值得推荐的中西医康复疗法.

  14. The dynamic observation of platelet parameters in children with mycoplasma pneumoniae pneumonia%肺炎支原体肺炎患儿血小板参数动态观察

    Institute of Scientific and Technical Information of China (English)

    吴起武; 赵萍

    2013-01-01

    目的 探讨肺炎支原体肺炎(MPP)患儿血小板参数和白细胞的变化及意义.方法 对300例MPP儿童(MPP组)在入院初及应用大环内酯类抗生素治疗1周后,采用全自动血细胞分析仪测定血小板参数[血小板计数(PLT)、血小板体积(MPV)、血小板分布宽度(PDW)]和白细胞计数(WBC),并与40例健康儿童(对照组)比较.结果 MPP组血小板增多(PLT> 300×109/L) 156例,发生率52.0% (156/300);血小板减少1例,发生率0.3% (1/300).入院初及治疗1周后MPP组WBC、MPV、PDW均高于对照组;治疗1周后PLT高于入院初和对照组,差异有统计学意义.结论 MPP患儿血小板增多症发生率高,对肺炎患儿进行血小板参数和WBC的动态观察,可用以作为肺炎支原体(MP)感染判断的初步依据.%Objective To investigate the changes and significance of platelet parameters and leucocyte in the children suffered from mycoplasma pneumoniae pneumonia (MPP).Methods The platelet parameters (PLT),the platelet volume(MPV),the platelet distribution width (PDW),and the white blood cell count(WBC) were detected by automatic blood cell analyzer respectively in 300 children with MPP before and after the treatment by macrolides antibiotics for a week as well as in 40 normal children.The comparison was made among them.Results There were 156 cases that suffered from thrombocytosis (PLT > 300 × 109/L) in MPP group,the incidence rate was 52.0%(156/300).While there was 1 case with thrombocytopenia,the incidence rate was 0.3% (1/300).The WBC,MPV and PDW in MPP group before and after one-week treatment were higher than the control group.And the PLT was higher than the initial stage of hospitalization and the control group.The differences had statistical significance.Conclusion The incidence rate of thrombocytosis was high in the children suffered from MPP.The dynamic observation of platelet parameters and WBC in children with MPP could be used as the elementary basis of judging

  15. Clinical characte ristics of mycoplasma pneumoniae pneumonia in children of different ages%不同年龄儿童肺炎支原体肺炎临床特征分析

    Institute of Scientific and Technical Information of China (English)

    唐迎元; 吴琼; 谭爱斌; 娄辉

    2014-01-01

    Objective To discuss mycoplasma pneumoniae pneumonia (MPP ) in children of different ages with clinical manifestations ,imaging features and change of laboratory data .Methods Retrospective analysis on 147 cases of MPP in children at age from 1 month to 14 years old in our hospital from January 2012 to July 2013 . Of all children with MPP there were 45 cases aged from 1 month to 3 years old and 102 cases above 3 years old . The clinical manifestation ,laboratory examination ,imaging manifestations and outcome were analyzed .Results 1 month-3 years old group has obvious respiratory symptoms ,cough and wheezing are severe ,some appeared res-piratory insufficiency ,but fever is not serious ,the image is given priority to with lobular parenchymal infiltration and interstitial lesions ,laboratory data lymphocyte percentage is relatively high .Above 3 years old group has high fever ,dry cough ,but rare respiratory insufficiency ,pulmonary segments or lung parenchyma lesions imaging per-formance more obvious .CRP is higher significantly .Two groups have significant differences in application rate of adrenal cortical hormone .The prognosis of all is good .Conclusion Clinical features in MPP children of different age is different ,may be associated with the different pathogenesis .%目的:探讨不同年龄肺炎支原体肺炎患儿的临床表现、影像学特征及实验室资料改变。方法回顾分析我院2012年1月~2013年7月确诊肺炎支原体肺炎患儿147例,按年龄分1月~3岁组45例,>3岁组102例。对其临床表现、实验室检查、影像表现、治疗转归进行分析。结果1月~3岁组呼吸道症状明显,咳嗽、喘息症状均较严重,部分出现呼吸功能不全,发热不严重,影像以小叶实质浸润及间质病变为主,实验室资料淋巴细胞比例相对偏高。>3岁组发热、干咳明显,但少见呼吸功能不全,影像表现以肺段或肺叶实质病

  16. 小儿支气管哮喘与肺炎支原体感染的关系%The relationship between children bronchial asthma and mycoplasma pneumoniae infection

    Institute of Scientific and Technical Information of China (English)

    仇世蓉

    2015-01-01

    目的:探讨小儿支气管哮喘与肺炎支原体(MP)感染的关系,从而为小儿支气管哮喘的治疗方案提供指导依据。方法:2009年6月-2013年6月收治呼吸道感染并支气管哮喘患儿100例作为观察组,另外100例呼吸道感染患儿作为对照组,通过血清学检查 MP-IgM,确定患儿肺炎支原体感染的情况。呼吸道感染并支气管哮喘组有48例MP-IgM阳性,将这48例患儿随机分为两组,一组按常规支气管哮喘治疗,另一组在常规治疗的基础上,加用阿奇霉素抗肺炎支原体感染治疗,比较两组患儿的治疗效果。结果:对照组的MP-IgM阳性率20%,观察组的MP-IgM阳性率48%,差异有统计学意义(P<0.05)。常规支气管哮喘治疗组有效率37.5%,常规支气管哮喘治疗加阿奇霉素治疗组有效率83.3%,差异有统计学意义(P<0.05)。结论:小儿肺炎支原体感染与支气管哮喘关系密切,对支气管哮喘患儿应常规检测肺炎支原体感染情况。阳性者在按哮喘常规治疗同时予抗肺炎支原体感染治疗,能提高疗效。%Objective:To discuss the relationship between children bronchial asthma and mycoplasma pneumoniae infection,so as to provide guidance for the treatment schedule of children bronchial asthma.Methods:100 bronchial asthma children with respiratory tract infection were selected from June 2009 to June 2013 as the observation group.100 children with respiratory tract infection were as the control group.The situation of children mycoplasma pneumoniae infection was ensured by serological examination MP-IgM.48 cases of MP-IgM in the respiratory tract infection with bronchial asthma group were positive.The 48 children were randomly divided into two groups.One group was treated with conventional treatment of bronchial asthma,and another group was treated with azithromycin against mycoplasma pneumoniae infection on the basis of conventional treatment.The treatment

  17. 血清降钙素原和C反应蛋白水平改变在支原体肺炎患儿中的临床意义%Changes of serum procalcitonin and C - reactive protein levels in children with mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    张月明

    2014-01-01

    目的:探讨血清降钙素原和 C 反应蛋白水平改变在支原体肺炎患儿中的临床意义。方法选择支原体肺炎、细菌性肺炎患儿各67例为支原体肺炎组和细菌性肺炎组,选择健康体检儿童67例为健康对照组。检测各组血清降钙素原(PCT)和 C 反应蛋白(CRP)水平并分析。结果支原体肺炎组急性期血清 PCT 水平显著高于健康对照组,但显著低于细菌性肺炎组( P 均<0.05)。支原体肺炎组、细菌性肺炎组血清 CRP 水平显著高于健康对照组( P <0.05),但两者 CRP 水平比较无显著差异。治疗后发现恢复期支原体肺炎组血清 PCT、CRP 水平显著下降,与健康对照组无统计学差异( P 均﹥0.05)。结论早期联合检测呼吸道感染患儿 PCT 与 CRP 水平可帮助早期鉴别支原体感染肺炎和细菌性感染肺炎,并可作为疗效观察的参考依据。%Objective To investigate the changes of serum procalcitonin(PCT)and C - reactive protein(CRP)levels in children with mycoplasma pneumonia. Methods Serum PCT and CRP levels in each group of children with mycoplasma pneumonia(n = 67)or bacterial pneu-monia(n = 67),and healthy children(n = 67)were detected and analyzed. Results In acute phase,serum PCT level was significantly higher in mycoplasma pneumonia group than healthy control group,but significantly lower than bacterial pneumonia group(all P < 0. 05). Serum CRP level in mycoplasma pneumonia group and bacterial pneumonia group was significantly higher than healthy control group( P < 0. 05),but no sig-nificant difference between mycoplasma pneumonia group and bacterial pneumonia group. After treatment,serum PCT and CRP levels were signifi-cantly decreased in children with mycoplasma pneumonia,but not significantly different from those of control group(all P ﹥ 0. 05). Conclusion Combined early detection of serum PCT and CRP for children with respiratory infection may help early identification

  18. 392例肺炎支原体抗体阳性患儿的临床分析%Clinical Analysis of 392 Cases of Children with Mycoplasma Pneumoniae Antibody Positive

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

      目的分析肺炎支原体( mycoplasma pneumoniae,MP)抗体阳性患儿的临床资料。方法收集392例MP阳性患儿的多项临床资料(年龄、临床表现、肺外症状、实验室检查、胸片、治疗与转归)进行回顾性分析。结果0~1岁患儿占总发病的16.33%、1~3岁34.44%、4~7岁43.37%、8~14岁5.87%;所有患儿都有咳嗽、66.00%的患儿有发热和5.36%有消化系统症状;阿奇霉素治疗1周后有效率92.35%,红霉素治疗1周后有效率72.65%,治疗2周后94.89%患儿症状消失。结论 MP感染与年龄有关,以1~7岁儿童高发,主要有咳嗽、发热及消化道症状,对阿奇霉素治疗较为敏感。%Objective To analyze mycoplasma pneumoniae, mycoplasma pneumoniae, MP) antibody positive patients clinical data. Methods To collect clinical data of 392 cases of children with MP positive multiple were retrospectively anal yzed. Results 0~1 year old children account for 16.33% of the total morbidity, 1~3 year oldchildren 34.44%, 4~7 year old children43.37%, 8-14 year old children5.87%; All children have a cough, 66.00% of children with fever and 5.36% had symptoms of digestive system; Azithromycin treatment effective rate was 92.35% after one week, one week after the erythromycin treatment effective rate was 72.65%, 94.89% in children with symptoms disappeared after 2 weeks treatment. Conclusion MP infection is with age is related to a high incidence of children 1-7 years old,mainly include coughing, fever and gastrointestinal symptoms, sensitive to azithromycin treatment.

  19. Meningoencephalitis associated with Mycoplasma pneumoniae

    Directory of Open Access Journals (Sweden)

    Isabella Batista de Lalibera

    2012-03-01

    Full Text Available We report a case of a child with meningoencephalitis of atypicaletiology. The patient developed the disease after an infection inthe upper airways with unfavorable evolution. The clinical recoverywas only possible after the administration of adequate antibiotictherapy for the etiological agent. This case report describes achild with meningoencephalitis of atypical etiology. The patientdeveloped the disease after an infection in the superior airways withnegative evolution. The clinical recovery was possible only after theintroduction of adequate antibiotic therapy for the etiological agent.

  20. 支原体肺炎患儿血常规检测诊断的应用%Application of blood routine examination in diagnosis of children with Mycoplasma pneumonia

    Institute of Scientific and Technical Information of China (English)

    周发为; 周维; 向慧; 游文忠

    2015-01-01

    OBJECTIVE To observe and compare the results of blood routine examination between the children with Mycoplasma pneumonia and the normal children ,analyze the difference ,and explore the efficacy of the blood rou‐tine examination in diagnosis of Mycoplasma pneumonia .METHODS Totally 50 children with Mycoplasma pneu‐monia who were treated from Jan 2013 to Jun 2013 were chosen as the observation group ,including 28 cases of males and 22 cases of females ,meanwhile ,50 normal children were set as the control group ,including 23 males and 22 females;the blood routine examinations were performed for the two groups of enrolled children ,and the differences in the monitoring indicators were analyzed .RESULTS The white blood cells counts and blood platelets counts of the observation group were higher than those of the control group ,and there was significant difference (P<0 .01) .The erythrocytes counts of the observation group were significantly lower than those of the control group .There was no significant difference in the hemoglobin counts between the two groups .The absolute value of neutrophils and mononuclear absolute value of the observation group were significantly higher than those of the control group (P<0 .01) .The absolute value of lymphocytes of the observation group was significantly lower than that of the control group (P<0 .01) .CONCLUSION The blood routine examination has certain value in diagnosis of the children with Mycoplasma pneumonia and facilitate the understanding of the illness condition of the chil‐dren ,and the operation of the method is simple ,time‐saving ,and economical .%目的:观察并对比支原体肺炎患儿与正常患儿的血常规检测结果,分析其差异性,探讨其在支原体肺炎的诊断效率。方法选取2013年1-6月收治的支原体肺炎患儿50例为观察组,其中男性28名、女性22名,另选正常儿童50名为对照组,其中男性23名、女性22名,分别对两组入选儿童进

  1. 异丙托溴铵、布地奈德雾化吸入加用口服急支糖浆辅助治疗小儿肺炎支原体肺炎的临床观察%Ipratropium Bromide Nebulized Budesonide Inhalation Plus Oral Acute Bronchitis Syrup Auxiliary Treatment of Mycoplasma Pneumoniae Pneumonia Clinical Observation

    Institute of Scientific and Technical Information of China (English)

    王丽珍; 李飞平; 罗芳; 张梅娟

    2012-01-01

    Objective; To study the budesonide, ipratropium bromide Stock aerosol inhalation plus oral acute bronchitis syrup efficacy of mycoplasma pneumonia in children. Method; from May 2011 to May 2012 in our hospital diagnosed with pneumonia, mycoplasma pneumonia, aged 3 to 14 years old, 69 cases of hospitalized children, 35 cases were randomly divided into a control group and a test group of 34 patients. Test group application budesonide, ipratropium bromide atomizing inhalation plus oral acute bronchitis syrup, observation and comparison of the two groups on the basis of given antibiotics. Experimental group, children with cough disappeared time, of pulmonary rales disappear and hospitalization time there was significantly shorter (P<0.01) than the control group, the total effective rate in the test group than the control group (P<0. 01). Conclusion:Budesonide, ipratropium bromide oxygen drive to the aerosol inhalation plus oral acute bronchitis syrup can be used as auxiliary treatment of mycoplasma pneumonia treatment.%目的:研究布地奈德、异丙托溴铵联合雾化吸入加用口服急支糖浆对小儿支原体肺炎的疗效.方法:将2011年5月-2012年5月在我院诊断为肺炎支原体肺炎,年龄3~14岁的69例住院患儿,随机分为对照组35例与试验组34例.在给予抗感染的基础上试验组应用布地奈德、异丙托溴铵氧气驱动雾化吸入加用口服急支糖浆,观察比较两组疗效.结果:试验组患儿咳嗽消失时间、肺部罗音消失时间及住院时间均比对照组有极显著缩短(P<0.01),试验组总有效率比对照组高(P<0.01).结论:布地奈德、异丙托溴铵氧气驱动雾化吸入加用口服急支糖浆可以作为治疗小儿支原体肺炎的辅助治疗方式.

  2. Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group.

    Science.gov (United States)

    Pettersson, B; Uhlén, M; Johansson, K E

    1996-10-01

    Almost complete (> 96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in Mycoplasma agalatiae, three were found in Mycoplasma bovis, one was found in Mycoplasma alkalescens, and one was found in Mycoplasma bovirhinis. The sequence data were used for construction of phylogenetic trees. All but one of the ruminant mycoplasmas sequenced in this work clustered in the hominis group. A close relationship was found between M. agalactiae and M. bovis, with a 99% nucleotide similarity between their 16S rRNA sequences. They were also found to be members of the Mycoplasma lipophilum cluster of the hominis group. Furthermore, the 16S rRNA comparisons showed that Mycoplasma alkalescens and Mycoplasma canadense are closely related (> 98.5%), and these species were found to cluster in the Mycoplasma hominis cluster of the hominis group. Interestingly, M. bovirhinis grouped in a new phylogenetic cluster of the hominis group. The new cluster, which was supported by bootstrap percentage values, signature nucleotide analysis, and higher-order structural elements, was named the Mycoplasma synoviae cluster. Mycoplasma bovoculi, Mycoplasma conjunctivae, and Mycoplasma ovipneumoniae clustered in the Mycoplasma neurolyticum cluster of the hominis group. Mycoplasma alvi clustered with Mycoplasma pirum in the M. pneumoniae cluster of the pneumoniae group.

  3. Chlamydia pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila in elderly patients with stroke (C-PEPS, M-PEPS, L-PEPS): a case-control study on the infectious burden of atypical respiratory pathogens in elderly patients with acute cerebrovascular disease.

    Science.gov (United States)

    Ngeh, Joseph; Goodbourn, Colin

    2005-02-01

    Multiple studies have suggested an association between Chlamydia pneumoniae and Mycoplasma pneumoniae infection and cardiovascular disease. We investigated whether the risk of cerebrovascular disease is associated with Legionella pneumophila infection and the aggregate number/infectious burden of these atypical respiratory pathogens. One hundred patients aged >65 years admitted with acute stroke or transient ischemic attack (TIA) and 87 control patients admitted concurrently with acute noncardiopulmonary, noninfective conditions were recruited prospectively. Using enzyme-linked immunosorbent assay (ELISA) kits, we previously reported the seroprevalences of C pneumoniae and M pneumoniae in these patients. We have now determined the seroprevalences of L pneumophila IgG and IgM in this cohort of patients using ELISA. The seroprevalences of L pneumophila IgG and IgM were 29% (n=91) and 12% (n=81) in the stroke/TIA group and 22% (n=86) and 10% (n=72) in the controls, respectively. Using logistic regression to adjust for age, sex, hypertension, smoking, diabetes, ischemic heart disease, and ischemic ECG, the odds ratios for stroke/TIA in relation to L pneumophila IgG and IgM were 1.52 (95% CI, 0.70 to 3.28; P=0.29) and 1.49 (95% CI, 0.45 to 4.90; P=0.51), respectively. The odds ratios in relation to IgG seropositivity for 1, 2, or 3 atypical respiratory pathogens after adjustment were 3.89 (95% CI, 1.13 to 13.33), 2.00 (95% CI, 0.64 to 6.21), and 6.67 (95% CI, 1.22 to 37.04), respectively (P=0.06). L pneumophila seropositivity is not significantly associated with stroke/TIA. However, the risk of stroke/TIA appears to be associated with the aggregate number of chronic infectious burden of atypical respiratory pathogens such as C pneumoniae, M pneumoniae, and L pneumophila.

  4. Clinical significance of combined detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in patients with community acquired pneumonia%社区获得性肺炎患者联合监测肺炎支原体与肺炎衣原体的临床意义

    Institute of Scientific and Technical Information of China (English)

    杨宏生; 许锡荣; 沈琪

    2016-01-01

    Objective To discuss the clinical significance of combined detection of Mycoplasma pneumoniae (Mp) and Chlamydia pneumoniae (Cp) in patients with community acquired pneumonia (CAP). Methods Mp and Cp were monitored in throat swab specimens from 1 580 patients with CAP admitted in our hospital from Feb. 2012 to Oct. 2014. The main pathogenic bacteria were cultured and identified, and the clinical effects of different anti-infective treat-ment plans were compared. Results Pathogens of CAP mainly included Mp (30.0%), Streptococcus pneumoniae (15.9%), Haemophilus influenzae (12.9%) and Cp (10.1%), and co-infection of 2 kinds of pathogens infection was com-mon (10.1%). In the treatment, the use frequency of β-Lactam antibiotics was the highest (76.71%). The cure rate ofβ-Lactam antibiotics combined with Macrolide antibiotics or Fluoroquinolone antibiotics (66.72%) was significantly higher than separate application of β-Lactam antibiotics (56.73%, P0.05), but the cure rate of β-Lactam antibiotics combined with Macrolide antibiotics or Fluoroquinolone antibiotics (69.7%) was significantly higher than separate application ofβ-Lactam antibiotics (44.4%) in the treatment of bacterial infection combined with Mp or Cp infection (P0.05),而在细菌合并Mp或Cp感染时,联合用药治愈率(69.7%)明显高于单用β-内酰胺类药物(44.4%),差异有统计学意义(P<0.05).结论 CAP患者感染Mp与Cp较多见,β-内酰胺类药物是常用治疗药物,β-内酰胺类联合大环内酯类或氟喹诺酮类药物可提高患者治愈率,Mp与Cp联合检测对于敏感抗生素的选择及疗效的提高具有重要临床意义.

  5. 支原体混合感染性患儿肺炎106例临床分析%Clinical Analysis of 106 Cases of Mycoplasma Mixed Infections in Children with Pneumonia

    Institute of Scientific and Technical Information of China (English)

    程力平; 孙捷; 柳克智

    2013-01-01

      目的:探讨支原体混合感染肺炎患儿的治疗措施及临床疗效.方法:本文选取了我院儿科病房2010年2月~2012年2月收住院治疗的106例MP肺炎病例,对患者进行标本采集和病毒检测,并对其临床表现和治疗进行分析.结果:支原体混合感染性肺炎均有咳嗽症状,部分患儿还存在发烧的情况.不同年龄段患儿支原体混合感染的肺炎的种类有所不同.106例MP肺炎患儿中有56例合并了其病原体感染(52.8%),其中合并病毒感染25例(23.6%),合并肺炎衣原体感染16例(15.1%),合并细菌感染15例(13.2%).检查出的病毒中,呼吸道合胞病毒10例(9.4%),副流感病毒1型8例(7.5%),腺病毒5例(5.7%),柯萨奇病毒1例(0.9%)、流感病毒A 1例(0.9%),流感病毒B、副流感病毒2型、副流感病毒3型未检出.痰培养阳性11例,分别是:肺炎克雷伯菌4例(3.8%)肺炎链球菌2例(1.9%),大肠埃希菌2例(1.9%),金黄色葡萄球菌2例(1.9%),鲍曼氏不动杆菌1例(0.9%),铜绿假单胞菌1例(0.9%).结论:MP合并其他病原体感染的儿童肺炎较单纯MP感染性肺炎症状更重,病程更长.在治疗方面,确诊MP 感染后,应尽快应用有特效的大环内酯类抗生素阿奇霉素治疗,如疗效不明显时,应考虑合并其他病原体感染,立即做病毒学检测、衣原体及痰培养检查,根据相应检查结果进一步治疗.%Objective:To investigate the therapeutic measures and clinical efficacy of Mycoplasma mixed infection in children with pneumonia.Methods:We selected the pediatric ward of our hospital from February 2010 to February 2012, 106 patients received hospital treatment MP pneumonia cases, the patient specimen collection and virus detection,analysis and its clinical manifestations and treatment. Results:Mycoplasma mixed infection pneumonia have cough, some children there is a fever.Different kind of different ages in children with mycoplasma

  6. E-selectin和sICAM-1在儿童肺炎支原体感染中的价值%Clinical value of E-selectin and sICAM-1 in diagnosis of children with Mycoplasma Pneumoniae infection

    Institute of Scientific and Technical Information of China (English)

    李苏亮; 叶芸

    2015-01-01

    目的 探讨E-选择素( E-selectin)与可溶性细胞间黏附分子-1 ( sICAM-1 )在儿童肺炎支原体感染中的应用价值.方法 选取本院儿科急性期肺炎支原体感染的患儿48例,恢复期患儿42例,选取同期体检的40例健康儿童做为对照组,采用ELISA法测定血浆E-selectin和sICAM-1水平. 用ROC曲线评价E-selectin和sICAM-1对急性期肺炎支原体感染的诊断效能;将急性期患儿血浆E-selectin与sICAM-1浓度进行相关性分析. 结果 急性期患儿血浆E-selectin和sICAM-1的水平明显高于恢复期及健康对照组,差异有统计学意义(P<0. 05);E-selectin诊断急性期肺炎支原体肺炎的AUC为0. 852(95%可信区间为0. 751-0. 952),其敏感度为80. 0%,特异度为78. 5%;sICAM-1 诊断急性期肺炎支原体肺炎的 AUC为0. 859 (95%可信区间为0. 764-0. 954),其敏感度为80. 5%,特异度为80. 0%. 急性期患儿E-selectin和sICAM-1水平呈明显正相关(r=0. 758,P<0. 01). 结论 急性期肺炎支原体感染患者血浆E-selectin和sICAM-1水平显著增高,可作为评价肺炎支原体感染病情程度以及治疗疗效观察的重要指标.%Objective To explore the diagnostic value of the E-selectin and sICAM-1 in children suffering from Mycoplasma pneumoniae infection. Methods Clinical data of 48 patients with acute Mycoplasma pneumoniae infection and 42 patients in recovery phase were reviewed, and 40 healthy children were chosen as control group. ELISA was used to detect the levels of E-selectin and sICAM-1, and the receiver operating characteristic(ROC) curve was used to evaluate the diagnosis efficiency of acute Mycoplasma pneumoniae infec-tion. The correlation between E-selectin and sICAM-1 was analyzed in patients with acute Mycoplasma pneumoniae infection. Results The levels of E-selectin and sICAM-1 were significantly higher in acute Mycoplasma pneumoniae infection than those in recovery phase(P<0. 05). The area under ROC curve of E-selectin was 0. 852(95%CI 0

  7. Chlamydophila felis: plasmid detection in Italian isolates.

    Science.gov (United States)

    Di Francesco, Antonietta; Donati, Manuela; Salvatore, Daniela; Cevenini, Roberto; Di Paolo, Maria; Baldelli, Raffaella

    2010-04-01

    Plasmids have been detected in the majority of strains in the genus Chlamydia and in many Chlamydophila species. Previous studies showed that FP Pring and FP Cello Chlamydophila felis strains have an extrachromosomial plasmid, whereas the FP Baker strain does not. Azuma et al. recently sequenced the entire genomic DNA sequence of the Japanese Cp. felis strain Fe/C-56 and described a 7,552 base pair circular plasmid. In the present study a highly conserved plasmid gene was detected in 11 Italian Cp. felis isolates, showing 100% nucleotide identity with the plasmid gene of Fe/C-56 Cp. felis strain.

  8. Study on correlation between serum IL-4,IL-5,IL-13 and complicating wheezing in children patients with Mycoplasma pneumoniae pneumonia%肺炎支原体肺炎患儿血清中白细胞介素水平与伴发喘息的相关研究

    Institute of Scientific and Technical Information of China (English)

    李梅; 陈正立; 李贵霞; 徐茜茹; 赵小洁

    2015-01-01

    目的:探讨肺炎支原体肺炎患儿血清中白细胞介素4(IL‐4)、白细胞介素5(IL‐5)、白细胞介素13(IL‐13)水平及其与喘息发生的关系。方法收集2013年1~10月123例肺炎支原体肺炎患儿,根据临床表现分为喘息组53例和无喘息组70例,喘息组根据临床病情分为急性期喘息组23例和缓解期喘息组30例,同时收集同期50例无喘息健康儿童血清作对照,采用酶联免疫吸附试验(ELISA)测定血清中IL‐4、IL‐5、IL‐13水平。结果肺炎支原体肺炎患儿血清中IL‐4、IL‐5、IL‐13水平高于健康对照组,喘息组患儿血清中IL‐4、IL‐5、IL‐13水平高于无喘息组患儿,血清IL‐4、IL‐5、IL‐13在急性期喘息组、缓解期喘息组中水平差异具有统计学意义(P<0.05)。患儿血清中IL‐4、IL‐5水平呈正相关( r=0.613,P<0.05),其余指标无相关性。结论血清IL‐4、IL‐5、IL‐13在肺炎支原体肺炎伴喘息患儿中水平升高,且伴随着喘息症状的加重水平上升,对肺炎支原体肺炎的治疗有指导意义。%Objective To investigate the relationship between serum interleukin‐4(IL‐4) ,interleukin‐5(IL‐5) and interleukin‐13(IL‐13) with complicating wheezing occurrence in children patients with Mycoplasma pneumoniae pneumonia .Methods 123 children cases of Mycoplasma pneumoniae pneumonia in our hospital from January 2013 to October 2013 were collected and divided into the wheezing group (53 cases)and the non‐wheezing group(70 cases) ac‐cording to the clinical manifestations .The wheezing group were re‐divided into the acute stage group(23 cases) and the remission stage group(30 cases) according to the clinical condition .Contemporaneous 50 healthy children without wheezing were collected as the control group .The serum IL‐4 ,IL‐5 and IL‐13 levels were measured by ELISA .Re‐sults The serum levels of IL‐4 ,IL‐5

  9. The Significance of Total IgE and C-reactive Protein of Children with Mycoplasma Pneumoniae Pneumonia%肺炎支原体肺炎患儿总IgE、C反应蛋白的变化及意义

    Institute of Scientific and Technical Information of China (English)

    刘建华

    2015-01-01

    Objective:To investigate the variation of total IgE and CRP of children with mycoplasma pneumoniae pneumonia in Shunyi District. Method:All subjects were selected in our hospital from Jan 2013 to Jan 2014,including MPP(n=80) and bacterial pneumonia(n=65) and healthy children(n=40) from January 2013 to January 2014.All children were drawed vein blood and analyzed MPP-IgM,total IgE and CRP.Result:Total IgE and CRP of MPP were higher than those of healthy children(P<0.001),and total IgE of MPP were higher than that of bacterial pneumonia(P<0.001).Conclusion:We should monitored CRP levels in early period of MPP.Enhancing immune function can improve MPP prognosis meanwhile regular anti-infection treatments.%目的:了解本地区肺炎支原体肺炎(mycoplasma pneumoniae pneumonia,MPP)患儿总IgE、C反应蛋白(C reactive protein,CRP)的水平变化,为临床治疗肺炎支原体肺炎提供指导。方法:选取2013年1月-2014年1月笔者所在医院收治的80例MPP患儿、65例细菌性肺炎患儿和40例健康体检的儿童,进行MPP抗体、总IgE和C反应蛋白测定,并进行比较分析。结果:MPP患儿总IgE、CRP高于健康体检儿童(P<0.001), MPP患儿总IgE高于细菌性肺炎患儿(P<0.001)。结论:MPP患儿早期应监测CRP变化,抗感染同时提高患儿免疫功能会改善患儿的预后。

  10. Validez de la reacción en cadena de la polimerasa para el diagnóstico de infecciones respiratorias producidas por Mycoplasma pneumoniae:meta-análisis

    Directory of Open Access Journals (Sweden)

    Olga Lucía Sarmiento

    2013-09-01

    Full Text Available Introducción. Mycoplasma pneumoniae es considerado un frecuente agente etiológico de infecciones respiratorias y de una amplia gama de manifestaciones extra-pulmonares. Pruebas diagnósticas con baja exactitud y validez llevaron a considerar la reacción en cadena de la polimerasa (PCR para el diagnóstico de M. pneumoniae. Objetivo: Evaluar la validez y la exactitud de la PCR para la detección de M. pneumoniae. Materiales y Métodos: Meta-análisis de estudios que evaluaron el diagnóstico de M. pneumoniae en tracto respiratorio por PCR, publicados en MEDLINE desde noviembre de 1966 hasta julio de 2009. El análisis estadístico incluyó: i cálculo de sensibilidad y especifi cidad de la PCR; ii prueba de homogeneidad de estimadores entre estudios; iii elaboración de summary receiver operating characteristic curves (SROC por un modelo de regresión lineal; iv método de Egger para evaluar sesgo de publicación. Resultados: De 44 estudios incluidos los cuales incluyeron 6111 pacientes se calculó una sensibilidad de 0,72 (rango 0,21-0,99 y una especifi cidad de 0,96 (rango 0,06-0,99. Estudios en los cuales se incluyeron solo niños la PCR mostró menor exactitud. Estudios con concentraciones de cloruro de magnesio mayores de 1,5 mM mostraron mayor exactitud. Conclusiones: Según los resultados de este estudio, la PCR no se recomienda como prueba rutinaria. Sin embargo, en casos con alta sospecha clínica de infección por M. pneumoniae en servicios de salud con alta prevalencia, la PCR es de utilidad. Estudios futuros deben reportar el espectro de la enfermedad y los resultados deben ser reportados por tipo de muestra y de acuerdo a los diferentes puntos de corte de la prueba de referencia utilizada.

  11. 88 cases of Mycoplasma pneumoniae infection in children after routine clinical research%88例小儿肺炎支原体感染后血常规临床研究

    Institute of Scientific and Technical Information of China (English)

    黎海东; 郑丹; 李仲庭

    2014-01-01

    目的:分析小儿肺炎支原体感染后血常规的变化规律。方法:选择88例支原体感染后的小儿为观察组,另选择同期80例体检健康小儿为对照组,对两组儿童的血常规检查结果进行分析对比。结果:两组小儿的血小板计数、白细胞计数、红细胞计数以及血红蛋白含量比较差异无统计学意义(P>0.05);中性粒细胞和单核细胞、淋巴细胞比例以及绝对值比较差异有统计学意义(P<0.05)。结论:对临床表现疑为肺炎支原体的,可通过对血常规的变化情况进行检测初步判断小儿视同存在支原体感染。%Objective Analysis of pediatric pneumonia mycoplasma infection after blood routine change rule.Methods Select 88 pediatric mycoplasma infection after set as the observation group,swap over the same period of 80 medical health children as control group,the two groups of children blood routine examination Results were analyzed.Results Two groups of children of platelet count,white blood cell count,and red blood cell count and hemoglobin content was no significantdifference(P>0.05);Neutrophils and monocytes,and lymphocytes percentage and absolute value has the obvious difference(P<0.05).Conclusion Through the changes of routine blood test can preliminary judgment pediatric inspect mycoplasma infection.

  12. Clinical risk factors for bronchial asthma after mycoplasma pneumoniae pneumonia in children%儿童肺炎支原体肺炎后发生支气管哮喘的临床危险因素分析

    Institute of Scientific and Technical Information of China (English)

    盛俊峰; 黄鹤

    2015-01-01

    Objective To investigate the clinical risk factors for bronchial asthma after Mycoplasma pneumoniae pneumonia ( MPP) in children.Methods Fifty-nine children with MPP who diagnosed as bronchial asthma during a 3-year follow-up were enrolled in the observation group.Sixty children with MPP who manifested without current cough,wheezing and chest distress after discharge were collected as control group.The histories of atopic dermatitis and allergy,family history of asthma,whether manifesting with wheezing,extra-pulmonary damage and large patchy shadow in lung,and eosinophilic granulocyte count,total serum IgE level when suffering MPP,the results of FEV1% and FEF25-75%3 months after discharge were retrospectively analyzed.The factors which might affect the incidence of bronchial asthma in children with MPP were assessed by univariate analysis and multivariate analysis.Results Univariate analysis revealed that the histories of atopic dermatitis and allergies,family history of asthma,FEF25-75%and total serum IgE level were related with the incidence of bronchial asthma in children with MPP(P<0.05).Multivariable logistic regression analysis revealed that the histories of atopic dermatitis and allergies, family history of asthma and total serum IgE level when suffered from MPP were the risk factors for the incidence of bronchial asthma in children with MPP,and FEF25-75%3 months after discharge was the protective factor(P<0.05).Conclusion The histories of atopic dermatitis and allergies,family history of asthma and total serum IgE level when suffer from MPP are the independent risk factors for the incidence of bronchial asthma in children with MPP,and FEF25-75%3 months after discharge is the protective factor.%目的 探讨儿童肺炎支原体肺炎( MPP)后发生支气管哮喘的临床危险因素. 方法 选择MPP且随访3年内诊断为支气管哮喘的患儿59例为观察组,另选取MPP患儿出院后无反复咳嗽、气喘、胸闷表现的60例为对照组. 回

  13. Evidence of Acute Mycoplasma Infection in a Patient with Incomplete and Atypical Kawasaki Disease: A Case Report

    Directory of Open Access Journals (Sweden)

    M. Ebrahim

    2011-01-01

    Full Text Available The etiology of Kawasaki disease remains unknown despite extensive studies. Some researchers suggest that it is caused by an infectious agent. This is a case report where a patient with incomplete Kawasaki disease was found to have evidence compatible with acute Mycoplasma pneumoniae infection. This is one of the several case reports linking Mycoplasma pneumoniae to Kawasaki disease as a possible trigger. This is perhaps due to a superantigen or is mediated by some other mechanism. Accurate and timely testing for Mycoplasma infections is difficult and has its limitations. Despite this, Mycoplasma pneumoniae should be considered in the differential and workup for Kawasaki disease.

  14. Diagnosis and treatment of 23 cases of Mycoplasma pneumonia complicated with embolism%支原体肺炎合并栓塞23例诊治分析

    Institute of Scientific and Technical Information of China (English)

    金尾静; 张维溪; 张海邻; 李昌崇

    2013-01-01

    目的 探讨儿童支原体肺炎合并栓塞的发生、临床特点及诊治.方法 对1990年1月至2012年12月国内外报道过的23例儿童支原体肺炎合并栓塞病例的临床资料进行回顾性分析.结果 23例支原体肺炎合并栓塞患儿年龄为4 ~ 13岁,男15例,女8例.19例单发栓塞,下肢静脉血栓4例,颈内动脉栓塞1例,脑梗死9例,心肌梗死2例,脾梗死2例,肺梗死1例;4例为多发栓塞,肺栓塞合并下肢深静脉栓塞2例,心肌梗死合并肺栓塞1例,大脑中动脉合并颈内动脉栓塞1例.8例有暂时性抗心磷脂抗体IgM阳性,其中2例合并蛋白C下降,1例合并蛋白S下降,而AT-Ⅲ缺乏1例.经过溶栓、抗凝治疗后,2例死亡,其余21例疗效良好.结论 肺炎支原体感染过程中存在高凝状态,具有血栓形成可能,尤其是具有血栓形成高危因素的患儿.早期诊断、积极抗凝、溶栓治疗是支原体肺炎合并栓塞诊治的关键.%Objective To explore the occurence,clinical characteristics and treatment of Mycoplasma pneumonia complicated with embolism in children.Methods Twenty-three cases with Mycoplasma pneumonia complicated with embolization were retrospectively analyzed from January 1990 to December 2012.Results The ages of cases were from 4 years old to 13 years old,and fifteen cases were male,eight cases were female.Nineteen cases with single-shot embolism included four cases of lower limb venous thrombosis,one case of internal carotid artery thrombosis,nine cases of cerebral infarction,two cases of cardiac infarction,two cases of splenic infarction,one case of pulmonary infarction; and the other four cases were multiple embolism,two cases combined pulmonary embolism and lower limb deep vein thrombosis,one case combined cardiac embolism and pulmonary embolism,one case combined internal carotid artery and the brain embolism.In addition,eight cases had temporary anti-cardiolipin antibody IgM,two cases combined protein C decrease,one case

  15. 个性化护理干预对肺炎支原体感染患儿康复效果的影响%The Impact of Individualized Nursing Intervention on Mycoplasma Pneumoniae Infection in Children’s Rehabilitation

    Institute of Scientific and Technical Information of China (English)

    孙烨; 赵贵娟

    2015-01-01

    Objective:To investigate the effects of individualized nursing intervention for Mycoplasma pneumoniae infection in children’s rehabilitation.Method:64 cases of Mycoplasma pneumoniae infection in children from April 2013 to April 2014 were randomly divided into 2 groups,32 cases of each group.The control group was given routine pediatric care, observation group was given individualized nursing intervention.Treatment effects and adverse reactions of the two groups were observed and statistically analyzed.Result:The treatment total effective rate of the observation group was 96.9%,significantly higher than the control group 78.1%, there was statistical significance(P<0.05).The incidence of adverse reactions of the observation group was 12.5%,significantly lower than the control group 50.0%(P<0.05). Conclusion:Personalized nursing intervention is a scientific and effective care program, which is conducive to the consolidation effect, reduce the incidence of adverse reactions, and promote early recovery of children, the condition for the recovery of Mycoplasma pneumoniae infection in children has a positive clinical significance.%目的:观察个性化护理干预对肺炎支原体感染患儿康复效果的影响。方法:将2013年4月-2014年4月64例肺炎支原体感染患儿均分为两组,每组32例。对照组接受儿科常规护理,观察组同时开展个性化护理干预。对比两组治疗效果以及不良反应情况,并对研究结果相关数据作统计学处理。结果:观察组患儿的治疗总有效率为96.9%,明显高于对照组的78.1%,差异有统计学意义(P<0.05);观察组患儿的不良反应发生率为12.5%,明显低于对照组的50.0%,差异有统计学意义(P<0.05)。结论:个性化护理干预是一种科学、有效的护理方案,其有利于巩固疗效,降低不良反应发生率,促进患儿早日康复,对肺炎支原体感染患儿的病情恢复具有积极的临床意义。

  16. Mycoplasma ovipneumoniae - A Primary Cause of Severe Pneumonia Epizootics in the Norwegian Muskox (Ovibos moschatus) Population

    DEFF Research Database (Denmark)

    Handeland, Kjell; Tengs, Torstein; Kokotovic, Branko

    2014-01-01

    high load of M. ovipneumoniae antigens within lung lesions, with particularly intensive staining in the neutrophils. Similar IHC finding were observed in archived lung tissue blocks from animals examined during the 2006 epidemic. An M. ovipneumoniae specific ELISA was applied on bio-banked muskox sera...... from stray muskoxen killed in the period 2004–2013 and sick muskoxen culled, as well as sera from wild reindeer (Rangifer tarandus tarandus) on Dovre and muskoxen from Greenland. Serology and mycoplasma culturing was also carried out on sheep that had been on pasture in the muskox area during...

  17. Study on the correlation between refractory asthma and Mycoplasma pneumoniae infection%难治性哮喘与肺炎支原体感染的关联研究

    Institute of Scientific and Technical Information of China (English)

    杨永清; 隋小芳; 费秀斌

    2015-01-01

    Objectives To investigate the relation of refractory asthma and Mycoplasma pneumoniae infection ( MP).Methods 45 patients of difficult asthma ( observation group) and 80 patients of bronchial asthma remission ( control group) hospitalized in our hospital from 2012 August to 2014 November were selected for the research, mycoplasma pneumonia IgM ( MP-IgM) antibody and serum total IgE levels of two groups were detected, through statistical analysis, correlation between the two diseases was ana-lyzed.Results The positive rate of MP-IgM antibody in observation group (66.7%) was significantly higher than the control group (33.8%);the difference was statistically significant (P<0.01).Serum total IgE level of MP-IgM positive patients with bronchial asthma was significantly higher than MP-IgM negative patients with bronchial asthma, two groups compared, the difference was sta-tistically significant (P<0.05).Conclusions MP infection and refractory asthma are closely related.%目的:探讨难治性支气管哮喘与肺炎支原体感染( MP)发病的关系。方法选取我院2012年8月至2014年11月住院治疗的难治性支气管哮喘患者(观察组)45例及支气管哮喘缓解期患者(对照组)80例,检测两组血清肺炎支原体IgM(MP-IgM)抗体及血清总IgE水平,通过统计学分析两者的相关性。结果观察组MP-IgM抗体阳性率(66.7%)显著高于对照组(33.8%),差异显著,有统计学意义(P<0.01;OR=3.93,95% CI:1.69~9.20);MP-IgM阳性的支气管哮喘患者血清总IgE平均水平高于MP-IgM阴性的支气管哮喘患者,两组比较差异显著,有统计学意义( P<0.05)。结论肺炎支原体感染与难治性支气管哮喘可能有密切的关联。

  18. Study on the inflammatory injury of lung tissue and influence on inflammatory cytokines in mouse induced by recombinant protein CPAF from Chlamydophila pneumoniae%肺炎嗜衣原体CPAF重组蛋白致小鼠肺组织炎症及对炎症细胞因子的影响

    Institute of Scientific and Technical Information of China (English)

    王华丽; 吴移谋; 郑江花; 朱翠明; 李忠玉; 周洲; 余敏君

    2011-01-01

    目的:研究肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn) 蛋白酶样活性因子(CPAF)的181~400aa基因(CPAFm)的重组蛋白在BALB/c小鼠体内引起的肺部炎症改变及对炎症细胞因子TNF-α和IL-6的水平的影响,为进一步探索CPAF在Cpn感染中的致病作用提供实验依据.方法:将纯化的CPAFm重组蛋白于鼻内或尾静脉注射BALB/c小鼠, HE染色观察肺组织病理形态学改变,计小鼠外周血与支气管肺泡灌洗液(Bronchoalveolar lavage fluids,BALF)中白细胞总数,ELISA方法检测血清和BALF中的TNF-α和IL-6的水平.结果:实验组BALB/c小鼠的肺组织出现中性粒细胞、淋巴细胞等炎症细胞浸润,对照组与正常组BALB/c小鼠肺组织未见明显病理改变.鼻内滴入重组蛋白实验组BALB/c小鼠BALF中白细胞总数明显高于GST对照组(P<0.05)、PBS对照组(P<0.05)和正常组(P<0.01);鼻内滴入和尾静脉注射重组蛋白实验组BALF中炎症因子IL-6、TNF-α的水平明显高于GST、PBS对照组与正常组(均P<0.01);鼻内滴入重组蛋白实验组外周血中炎症因子IL-6和TNF-α水平显著高于GST、PBS对照组和正常组(P<0.05);尾静脉注射重组蛋白实验组外周血中炎症因子IL-6的水平明显高于相应的对照组(均P<0.01).结论:CPAFm重组蛋白有致病性,能引起BALB/c小鼠肺组织的炎症损伤和炎症因子TNF-α、IL-6的水平的升高,肺损伤与BALB/c小鼠体内炎症细胞增多和炎症因子TNF-α、IL-6的水平的升高相关.%Objective :To provide experimental basis for exploring pathogenic mechanism of Chlamydophila pneumoniae ,and to investigate the changes of pulmonary inflammation and inflammatory cytokines inclucling TNF-α and IL-6 levels in mouse model .Methods :The gene fragment of Chlamydial protease-like activity factor(CPAF)from C .pneumoniae (CPAFm ) was cloned , which encoded 181~400aa of the protein .The gene fragment was inserted into plasmid (name) and expressed in

  19. Early diagnosis using recombinant protein of immunodominant region gene of chlamydial protease-like activity factor from Chlamydophila pneumoniae%肺炎嗜衣原体蛋白酶样活性因子免疫优势区基因重组蛋白在早期诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    郑江花; 吴移谋; 刘佳强; 刘广超; 陈超群

    2008-01-01

    [目的]克隆和表达肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)蛋白酶样活性因子(CPAF)免疫优势区基因,评价重组蛋白在早期感染诊断中的应用价值.[方法]挑选并克隆出Cpn CPAF免疫优势区基因,构建原核表达载体,诱导表达并纯化重组蛋白,分析其抗原特异性;间接ELISA法检测Cpn参考血清、临床血清标本中的特异性IgM抗体,以及呼吸道感染患者痰咽拭子中的Cpn抗原;检测沙眼衣原体(Chlamydia trachomatis,Ct)临床阳性血清和泌尿生殖道分泌物.[结果]高效表达和纯化出一相对分子量约51.3kDa的重组蛋白;Western blot证明其只与人抗Cpn抗血清发生特异性反应;间接ELISA法检测40份Cpn IgM参考血清,阴性和阳性结果的一致率均为100%(40/40);与"金标准"方法MIF对照,检测300例临床血清标本中的IgM抗体,符合率为98.3%;与PCR试剂对照,检测120份呼吸道感染患者痰咽拭子中的Cpn抗原,符合率为88.3%;检测Ct阳性血清和泌尿生殖道分泌物,与Ct没有交叉反应.[结论]制备的CPAF免疫优势区基因重组蛋白具有良好的抗原性,在Cpn感染早期诊断中具有较高的利用价值.

  20. In-Depth Analysis of Lower Respiratory Tract in Children with Pneumonia Mycoplasma Infection the Clinical Effect of Treatment%深入分析小儿下呼吸道肺炎支原体感染治疗的临床效果

    Institute of Scientific and Technical Information of China (English)

    余平

    2015-01-01

    目的:探讨小儿下呼吸道肺炎支原体感染的临床诊断与治疗方法,并深入分析小儿下呼吸道肺炎支原体感染的临床治疗效果。方法通过研究小儿下呼吸道肺炎支原体感染的发病机制、临床表现、诊断、治疗与预后的整个过程,针对2012年5月到2013年6月的110例检出肺炎支原体感染的儿童患者,采用临床血清检测为主要诊断方法,大环内酯类药物为治疗方法,深入分析分析小儿下呼吸道肺炎支原体感染的治疗的临床效果。结果临床诊断结果显示患者在患病早期临床表现主要为发热,持续性发热且伴有剧烈的咳嗽,外周血白细胞和CRP的检测结果大部分正常,肺炎支原体(Mycoplasma pneumoniae)抗原特异性抗体IgM的检测结果为阳性。治疗结果显示大环内酯类抗生素治疗肺炎支原体感染效果良好,大部分病例采用阿奇霉素进行治疗。结论小儿下呼吸道肺炎支原体感染因为患者年龄较小,临床诊断有一定难度,单采用X光检测易于病毒性及细菌性肺炎混淆,必须采用血清学检测的手段,尤其是对肺炎抗原性抗体IgM的检测。治疗多采用阿奇霉素等大环内酯类药物。临床中时有不典型病例的出现,且不典型病例呈逐渐增加的趋势,因此诊断治疗过程中应做到及时多方面的准确诊断。%ABSTRAT:Objective to studythe lower respiratory tract in children with mycoplasma pneumoniae infection in clinical diagnosis and treatment, and in-depth analysis of lower respiratory tract in children with mycoplasma pneumoniae infection of clinical therapeutic effect. Methods by studying the lower respiratory tract in children with mycoplasma pneumoniae infection of pathogene-sis, clinical manifestation, diagnosis, treatment and prognosis of the entire process, for 2012 years until June of 2013 of 110 cases of detection of mycoplasma pneumoniae infection children patients

  1. Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics of pig lines generated by crossing an MPS pulmonary lesion selected Landrace line and a highly immune capacity selected Large White line.

    Science.gov (United States)

    Borjigin, Liushiqi; Shimazu, Tomoyuki; Katayama, Yuki; Watanabe, Kouichi; Kitazawa, Haruki; Roh, Sang-Gun; Aso, Hisashi; Katoh, Kazuo; Uchida, Takafumi; Suda, Yoshihito; Sakuma, Akiko; Nakajo, Mituru; Suzuki, Keiichi

    2016-08-01

    To understand the influence of crossbreeding on Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics, two crossbred lines were characterized. One crossbred line, LaWa, was generated by crossing the MPS pulmonary lesion selected Landrace line (La) and the highly immune-selected Large White line (Wa). The second crossbred line, LaWb, was generated by crossing the La line and the nonselected Large White line (Wb). The crossbred LbWb line (nonselected Landrace line × nonselected Large White line) and the La line were used as controls. The LaWa and LaWb lines had an intermediate level of MPS lung lesions between La and LbWb lines, although the difference was not statistically significant. After stimulation with sheep red blood cells (SRBCs), the LaWb and LaWa lines showed immune characteristics similar to that of the La line; the number of monocytes in peripheral blood increased, while B cells, T cells, secretion of SRBC-specific immunoglobulin G, and interleukin (IL)-13 decreased. Additionally, the number of natural killer (NK) cells and the expression of IL-4 and IL-17 were significantly higher in the LaWb and LaWa lines, respectively. These data suggested that crossbreeding of La and Wa lines resulted in the inheritance of some of the selected immune responses. © 2015 Japanese Society of Animal Science.

  2. Perfil clínico, epidemiológico e etiológico de pacientes com pneumonia adquirida na comunidade internados em um hospital geral da microrregião de Sumaré, SP Clinical, epidemiological, and etiological profile of inpatients with community-acquired pneumonia at a general hospital in the Sumaré microregion of Brazil

    Directory of Open Access Journals (Sweden)

    Maria Rita Donalisio

    2011-04-01

    Full Text Available OBJETIVO: Analisar aspectos clínicos, etiológicos e epidemiológicos das pneumonias adquiridas na comunidade (PAC em indivíduos internados. MÉTODOS: Foram estudados prospectivamente 66 pacientes com PAC maiores de 14 anos no Hospital Estadual Sumaré, localizado na cidade de Sumaré (SP, entre outubro de 2005 e setembro de 2007. Coletamos dados sobre história clínica, exame clínico, escore pneumonia severity index (PSI e exames laboratoriais (hemocultura, bacterioscopia/cultura de escarro, sorologias para Chlamydophila pneumoniae, Mycoplasma pneumoniae e Legionella pneumophila, além de antígenos urinários de Legionella sp. e Streptococcus pneumoniae. RESULTADOS: A idade média dos pacientes foi de 53 anos, a maioria tinha baixa escolaridade, e 55,7% apresentavam pelo menos uma comorbidade no momento da internação. O percentual de idosos vacinados contra influenza entre os internados foi significativamente menor que os da comunidade dos municípios da microrregião de Sumaré (52,6% vs. > 70%. A febre foi menos frequente entre os idosos (p OBJECTIVE: To analyze the clinical, etiological, and epidemiological aspects of community-acquired pneumonia (CAP in hospitalized individuals. METHODS: We prospectively studied 66 patients (> 14 years of age with CAP admitted to the Hospital Estadual Sumaré, located in the Sumaré microregion of Brazil, between October of 2005 and September of 2007. We collected data related to clinical history, physical examination, pneumonia severity index (PSI scores, and laboratory tests (blood culture; sputum smear microscopy and culture; serology for Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila; and detection of Legionella sp. and Streptococcus pneumoniae antigens in urine. RESULTS: The mean age of patients was 53 years. Most had a low level of education, and 55.7% presented with at least one comorbidity at the time of hospitalization. The proportion of elderly people vaccinated

  3. Difference in Susceptibility to Mycoplasma Pneumonia Among Various Pig Breeds and Its Molecular Genetic Basis%猪支原体肺炎发生的品种敏感差异及分子基础

    Institute of Scientific and Technical Information of China (English)

    方晓敏; 赵为民; 付言峰; 涂枫; 李碧侠; 王学敏; 赵芳; 任守文

    2015-01-01

    allocated randomly into the treatment group (15 pigs of each breed) or control group (5 pigs of each breed). The pigs of treatment group were administered withM. hyopneumoniae strain JS. Meanwhile, the control pigs were injected with physiological saline solution. The treatment and control groups were isolated and fed an antibiotic-free diet throughout the experimental period. And then, clinical symptoms were observed daily, and average daily weight gain, antibodies level, X-ray transmission andM. hyopneumoniae pathogen were detected at 18 and 28 days postM. hyopneumoniae challenge, respectively. Twenty-eight days post challenge, the pigs were sacrificed and inspected for hepatization of the lung lobes as an indicator of Mycoplasma pneumonia. Then, 2 infected individuals and 2 non-infected individuals of each breed pigs byM. hyopneumoniae were selected to perform Agilent Pig 4x44K Gene Expression Microarray analysis, and the double comparison method of inter- and intra-breed was used to investigate differential expression genes. Furthermore, Gene Ontology (http://www. geneont ology. org) and KEGG pathway (http://www. genome.jp/kegg/) were applied to analyze the roles of these differentially expressed genes involved in signaling pathway and regulatory network to discuss the molecular genetic basis in the process ofM. hyopneumoniae infection in swine.[Result]The results showed that, from day 1 to day 18 of experiment period, the average daily weight gain of the treated pigs was significantly lower than that of the control pigs (0.01≤P<0.05), especially for Meishan pigs. From day 19 to day 28, negative growth (an average daily weight gain of less than 0.00 kg) and a drop in average daily weight gain were recorded for pigs in the treatment group. And the average daily weight gain of treated pigs was significantly lower than that of the control pigs (P<0.01). Eighteen days postM. hyopneumoniaechallenge, the antibody level of Landrace pigs and Suzhong pigs had little change and

  4. Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing.

    Science.gov (United States)

    Wolff, Bernard J; Benitez, Alvaro J; Desai, Heta P; Morrison, Shatavia S; Diaz, Maureen H; Winchell, Jonas M

    2017-03-01

    We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations.

  5. Significance of Th17 and IL-17 in detecting mycoplasma pneumonia%Th17和IL-17在肺炎支原体肺炎患儿中的检测意义

    Institute of Scientific and Technical Information of China (English)

    叶建兰

    2013-01-01

    Objective To investigate the clinical value of serum interleukin-17 (IL-17) and peripheral blood T helper cells (Th17) in Mycoplasma pneumoniae pneumonia (MPP).Methods 268 cases of Mycoplasma pneumoniae (MP) infection in children were selected for the study.100 cases of healthy children were selected as the control group.IL-17 and Th17 in peripheral blood were detected by ELISA and flow cytometry.The IL-17 level and Th17 frequency of different positive antibodies children and children with different clinical symptoms were analyzed.Results IL-17 level and Th17 frequency were (11.68±4.36) ng/L and (2.42±0.53)% in positive MpIgM group,(17.39±7.23) ng/L and (3.18±0.62)% in positive MpIgM+IgG group,(6.58±1.63) ng/L and (1.22±0.39)% in control group.The pairwise comparison difference was statistically significant (P <0.05).There was statistical significance in IL-17 and Th17 in children with different clinical manifestations.Conclusions The pathogenesis of MPP is closely related to IL-17 level and Th17 frequency,which can be used as the evaluation indexes of progression and outcome of MPP in children.%目的 探讨血清中白介素17(IL-17)以及外周血辅助性T细胞(Th17)在肺炎支原体肺炎(MPP)患儿中的临床应用价值.方法 选取确诊为肺炎支原体(MP)感染患儿268例为研究对象,同时选择100名健康儿童为对照组.分别采用ELISA法以及流式细胞术检测受试者血清中IL-17以及外周血Th17的含量,对比分析不同临床症状患儿体内IL-17、Th17差异.结果 MpIgM阳性患儿体内IL-17、Th17分别为(11.68±4.36) ng/L、(2.42±0.53)%,MpIgM+ IgG阳性患儿体内IL-17、Th17分别为(17.39±7.23) ng/L、(3.18±0.62)%,对照组IL-17、Th17分别为(6.58±1.63) ng/L、(1.22±0.39)%,两两比较差异有统计学意义(P<0.05).不同临床表现的患儿体内IL-17、Th17存在差异性.结论 MPP的发病机制与IL-17水平以及Th17频数异常有密切关系,两者可作为MPP患儿病情发展、转归的评价指标.

  6. A comparative analysis of two different methods for detection of Mycoplasma pneumoniae infection in children%两种不同方法检测儿童肺炎支原体感染的对比分析

    Institute of Scientific and Technical Information of China (English)

    李正秋; 刘兰香; 刘云花

    2016-01-01

    Objective To compare the serum Mycoplasma pneumoniae antibody ELISA test with indi-rect blood coagulation test method,and to explore the high cost-effective method in laboratory diagnosis of Mycoplasma pneumoniae infection.Methods Totally 352 cases of bronchial pneumonia in children hospital-ized in Department of Paediatrics of Children's Hospital of Hunan Province from December 2013 to January 201 5 were included,and respiratory secretions and serum were made ELISA and indirect hemagglutination test,and serum was isolated and cultured.The separate culture was regarded as the gold standard for the detection.ELISA and indirect hemagglutination test were compared on diagnosis effect.Results The sensi-tivity of indirect hemagglutination test method was 97.5% (1 59/1 63 ),significantly higher than that of ELISA(86.5%,141/1 63)(P <0.05);The specificity of ELISA method for the detection was 91.5%(1 73/189),significantly higher than indirect hemagglutination method(70.9%,134/189 ),the difference being statistically significant(P <0.05 ).The diagnostic efficiency of ELISA detection method was 89.2%,the Kappa index being 0.782;the diagnostic efficiency of indirect hemagglutination method was 83.2%,and the Kappa index was 0.670.The diagnostic efficiency of the two methods was similar,and the Kappa index was in a high degree of consistency.Conclusion ELISA test and indirect blood coagulation test method have their own advantage,so the laboratory can choose the appropriate method according to the clinical diagnosis.%目的:比较血清肺炎支原体抗体 ELISA 检测和间接血凝试验法,探讨肺炎支原体感染实验室诊断的高性价比方法。方法2013年12月至2015年1月湖南省儿童医院儿科收治住院的支气管肺炎患儿352例,取患儿呼吸道分泌物及血清进行 ELISA 检测和间接血凝试验,并且对血清进行分离培养,以分离培养作为检测的金标准,比较 ELISA 检测和间接血凝试验的诊断效果。

  7. 实时荧光定量-聚合酶链反应方法检测肺炎支原体%A real-time PCR assay for detection of Mycoplasma pneumoniae

    Institute of Scientific and Technical Information of China (English)

    孟凡亮; 何利华; 顾一心; 张建中; 赵飞

    2013-01-01

    目的 建立一种快速、灵敏、特异的肺炎支原体实时荧光定量-聚合酶链反应(real-time PCR)检测方法,以期用于临床肺炎支原体感染检测.方法 通过测序分析和序列比对,选取肺炎支原体p1基因中保守区域设计特异性引物和荧光探针,建立和完善此real-time PCR检测方法,并进行扩增效率、灵敏度及特异度评价.与已报道的肺炎支原体常规聚合酶链反应(PCR)方法进行150份临床标本检测能力比较.结果 建立的real-time PCR方法对肺炎支原体的检测限约为10 cfu.使用该方法对9株肺炎支原体ATCC标准株和30株临床分离株核酸扩增均为阳性;对10种其他支原体、13种常见呼吸道病原菌染色体及人类染色体扩增结果均为阴性.同时,临床标本的检测结果显示该方法检测灵敏度优于常规PCR.结论 本研究建立的real-time PCR方法可快速、灵敏、特异地检测标本中肺炎支原体核酸,可适用于临床肺炎支原体诊断.%Objective To established a simple, rapid real-time PCR assay to detect Mycoplasma pneumoniae in clinical specimens. Methods By analyzing the pl gene sequence of M. pneumoniae isolates, an optimized real-time PCR assay was designed. The specificity and sensitivity of this assay were evaluated and compared with conventional PCR assay using clinical specimens. Results The detection limit of the real-time PCR assay was about 10 cfu. The sensitivity and specificity of the assay were high. This real-time PCR assay was superior to conventional PCR in clinical specimen detection. Conclusion The real-time PCR assay is suitable for the detection of M. pneumoniae in clinical specimens.

  8. MYCOPLASMA INFECTION IN CHILDREN: CURRENT DIAGNOSIS AND TREATMENT

    Directory of Open Access Journals (Sweden)

    F. S. Harlamova

    2016-01-01

    Full Text Available Presents literary and own data (52 patients on the role of mycoplasma infection. Mycoplasma infection is associated with long-term antigenemia in children, causes recurrent disease and autoimmunity. Among the 52 examined patients diagnosed with pneumonia in 17, bronchitis — from 19, rhinosinusitis  — at 11, StevensJohnson syndrome — in 2 children.  In half the cases mycoplasmosis occurs against the backdrop of persistent active herpes virus infection (in 27 children (52%. To optimize the causal and pathogenetic therapy mycoplasma requires correction of immune disorders.

  9. Cloning and expression of Cpn0425 recombinant protein from Chlamydophila pneumoniae and its antigenicity%肺炎嗜衣原体Cpn0425重组蛋白的克隆与表达及抗原性研究

    Institute of Scientific and Technical Information of China (English)

    肖光文; 刘良专; 徐磊; 谢小平; 吴移谋

    2012-01-01

    The aim of this study is to construct the rccombinant protein of Cpn0425 from Chlamydop hila pneumoniae and induce its expression in E. coli, meanwhile assess the antigenicity of protein Cpn0425 . The gene of Cpn0425 was amplified by PCR , which was used to construct the recombinant plasm id of pGEX6p-2/Cpn0425 , and then the products were identified and analyzed by SDS-PAGE and Western blot . The restriction enzymes cleavage analysis and nncleotide sequencing showed the target gene was successfully inserted into pGEX 6p-2 prokaryotic expression vector . The similarity was 100% between the in-serted gene and Cpn0425 gene reported in GenBank by BLAST analysis . The SDS-PAGE demonstrated that the GST-Cpn0425 recombinant protein with a relative molecular weight about 50 kDa was expressed after the E. coli BL21 contained recombinant plasmid was induced and mainly existed in the pattern of solubility . Its purity reached up to 95% after purification with gluta-thione S-transferase (GST) resin chromatography of Novagen . In conclusion , we obtain Cpn0425 gene of Gt and express it suc-cessfully in E. coli BL21 . The GST-Cpn0425 has a good antigenicity.%目的 构建重组质粒pGEX6p-2/Cpn0425,在E.coli BL21中进行诱导表达,纯化获得重组融合蛋白Cpn0425,并评价其抗原性.方法 PCR扩增肺炎嗜衣原体Cpn0425蛋白编码基因,构建pGEX6p-2/Cpn0425重组质粒,测序正确后在E.coli BL21中诱导表达,用SDS-PAGE和 Western-Blot 进行分析和鉴定.结果 构建了重组质粒pGEX6p-2/Cpn0425,并在E.coli BL21菌中高效表达出Mr约为50kDa的GST-Cpn0425目的 蛋白,超声裂解后SDS-PAGE分析目的 蛋白在菌体细胞内以可溶性形式表达,经采用默克Novagen的GST纯化树脂纯化获得纯度在95%以上的重组蛋白.结论 获得了Cpn0425基因片段,并在E.coli BL21中成功表达,GST-Cpn0425具有良好的抗原性.

  10. Evaluation of Mycoplasma inactivation during production of biologics: egg-based viral vaccines as a model.

    Science.gov (United States)

    David, Selwyn A Wilson; Volokhov, Dmitriy V; Ye, Zhiping; Chizhikov, Vladimir

    2010-05-01

    Although mycoplasmas are generally considered to be harmless commensals, some mycoplasma species are able to cause infections in pediatric, geriatric, or immunocompromised patients. Thus, accidental contamination of biologics with mycoplasmas represents a potential risk for the health of individuals who receive cell-derived biological and pharmaceutical products. To assess the efficiency of inactivation of mycoplasmas by the agents used in the manufacture of egg-derived influenza vaccines, we carried out a series of experiments aimed at monitoring the viability of mycoplasmas spiked into both chicken allantoic fluid and protein-rich microbiological media and then treated with beta-propiolactone, formalin, cetyltrimethylammonium bromide, Triton X-100, and sodium deoxycholate, which are agents that are commonly used for virus inactivation and disruption of viral particles during influenza vaccine production. Twenty-two mycoplasma species (with one to four strains of each species) were exposed to these inactivating agents at different concentrations. The most efficient inactivation of the mycoplasmas evaluated was observed with either 0.5% Triton X-100 or 0.5% sodium deoxycholate. Cetyltrimethylammonium bromide at concentrations of >or=0.08% was also able to rapidly inactivate (in less than 30 min) all mycoplasmas tested. In contrast, negligible reductions in mycoplasma titers were observed with 0.0125 to 0.025% formaldehyde. However, increasing the concentration of formaldehyde to 0.1 to 0.2% improved the mycoplasmacidal effect. Incubation of mycoplasmas with 0.1% beta-propiolactone for 1 to 24 h had a marked mycoplasmacidal effect. A comparison of the mycoplasma inactivation profiles showed that strains of selected species (Mycoplasma synoviae, Mycoplasma gallisepticum, Mycoplasma orale, Mycoplasma pneumoniae, and Acholeplasma laidlawii) represent a set of strains that can be utilized to validate the effectiveness of mycoplasma clearance obtained by inactivation and

  11. [Pneumonia: The urgent problem of 21st century medicine].

    Science.gov (United States)

    Chuchalin, A G

    2016-01-01

    The paper analyzes the systematic reviews and meta-analyses on the strategic issues of pneumonia, which have been published in the past 3 years. It discusses the prevalence and mortality rates of acquired pneumonia, hospital-acquired (nosocomial) pneumonia, healthcare-associated pneumonia, ventilator-associated pneumonia, and Mycoplasma pneumonia, and the specific features of their etiology, diagnosis, and treatment. A large number of investigations emphasize the relevance of this problem in current clinical practice.

  12. Value of laboratory indicators for clinical diagnosis and treatment of the patients with severe hand, foot and mouth disease complicated with Mycoplasma pneumoniae infection%重症手足口病患儿合并肺炎支原体感染实验室检测指标对于临床诊断及治疗的提示性作用

    Institute of Scientific and Technical Information of China (English)

    曲沛; 徐新民; 赵苗苗; 李娟; 宋淑静; 郭杰; 华文浩

    2015-01-01

    Objective To investigate the value of laboratory indicators in clinical diagnosis and treatment of the patients with severe hand, foot and mouth disease(HFMD) commplicated with enterovirus 71(EV71) and Mycoplasma pneumoniae infection. Methods The data of 100 cases with HFMD and 50 cases with non-HFMD were collected in Beijing Ditan Hospital from March 2014 to August 2014. Real-time fluorescence quantitative PCR( RT-PCR) method was used to test the DNA in stool samples and obtained qualitative results. Passive agglutination test was performed to detect Mycoplasma pneumoniae antibody titers in serum. Then, we analyzed the possibility of Mycoplasma pneumoniae infection in HFMD group and non-HFMD group, and explored the correlation of EV71 infection complicated with Mycoplasma pneumoniae infection and the severity of HFMD. Results The positive rate of Mycoplasma pneumoniae infection in the severe HFMD group(76%) was significantly higher than that in the mild HFMD group(48%, χ2=8. 319, P=0. 004). Conclusion Close teention should be paid to severe HFMD patients with the EV71 infection complicated with Mycoplasma pneumoniae infection. This is critically important for reducing the severity of HFMD.%目的:探讨肠道病毒71型合并肺炎支原体感染的重症手足口患儿的检测指标对于临床诊断及治疗是否起到提示作用。方法收集2014年3月至8月于首都医科大学附属北京地坛医院住院的手足口病患儿100例,非手足口病患儿50例,对其粪便标本采用荧光定量RT-PCR方法检测,得出核酸定性结果。对其血清标本采用被动凝集法检测以得出肺炎支原体抗体效价。分析肺炎支原体感染在手足口病组和非手足口病组中的差异,探讨肠道病毒71型合并肺炎支原体感染与手足口病严重程度的相关性。结果重症手足口病组患儿的肺炎支原体阳性率(76%)高于轻症手足口病组患儿的肺炎支原体阳性率(48%),差异有统计学意义(χ2

  13. Antimicrobial susceptibility of Mycoplasma pneumoniae and drug resistance mechanisms to macrolides%肺炎支原体对抗菌药物敏感性及对大环内酯类的耐药机制研究

    Institute of Scientific and Technical Information of China (English)

    赵茂茂; 宋波; 蒲增惠; 于红霞

    2014-01-01

    OBJECTIVE To investigate the prevalence of Mycoplasma pneumoniae infection in the adults and teenagers with community-acquired pneumonia (CAP) ,observe the drug susceptibility to the commonly used antibiotics ,and define the drug resistance mechanisms to the macrolides .METHODS From Oct 2010 to Mar 2012 , the throat swab specimens were collected during the acute stage to isolate and culture the M .pneumoniae .The molecular identification of the clinical isolates was performed by using polymerase-chain-reaction , the minimal inhibitory concentrations (MICs) of macrolides ,quinolones ,and tetracyclines were determined with the use of microdilution method .The 23S rRNA gene sequencing was performed for the macrolides-resistant strains and was compared with the gene sequencing of the standard strain MPFH (ATCC 15531) .RESULTS A total of 20 strains of M .pneumoniae have been isolated from the throat swab specimens obtained from the 129 CAP patients ,with the isolation rate of 15 .50% ;the strains were highly susceptible to tetracyclines and fluoroquinolones and were resistant to all the macrolides ,and both of the resistance mechanisms were the mutation of locus 2063 of 23 rRNA from A to G .CONCLUSIONS The situation of the drug resistance of M .pneumoniae to macrolides is harsh ,and the mutation of locus of the 23S rRNA is the major drug resistance mechanism .%目的:了解成人及青少年社区获得性肺炎(CAP)中肺炎支原体(MP)感染状况及其对常用抗菌药物的敏感性,明确M P对大环内酯类的耐药机制。方法2010年10月-2012年3月129例C A P患者取急性期咽拭子标本进行肺炎支原体分离培养,应用聚合酶链反应(PC R )对临床分离株进行分子鉴定;采用微量稀释法测定肺炎支原体对大环内酯类、喹诺酮类及四环素类抗菌药物的最低抑菌浓度(M IC );对大环内酯类耐药株进行23S rRNA 基因测序,并与标准菌株MPFH (ATCC 15531)基

  14. Key role of Chlamydophila psittaci on Belgian turkey farms in association with other respiratory pathogens.

    Science.gov (United States)

    Van Loock, M; Geens, T; De Smit, L; Nauwynck, H; Van Empel, P; Naylor, C; Hafez, H M; Goddeeris, B M; Vanrompay, D

    2005-04-25

    Two hundred turkey sera from eight Belgian and two French farms were tested for the presence of antibodies against avian pneumovirus (APV), Ornithobacterium rhinotracheale (ORT), Mycoplasma gallisepticum, Mycoplasma meleagridis and Chlamydophila psittaci. At slaughter, C. psittaci, APV and ORT antibodies were detected in 94, 34 and 6.5% of the turkeys, respectively. No antibodies against M. gallisepticum or M. meleagridis were present. Additionally, turkeys on three Belgian farms were examined from production onset until slaughter using both serology and antigen or gene detection. All farms experienced two C. psittaci infection waves, at 3-6 and 8-12 weeks of age. Each first infection wave was closely followed by an ORT infection starting at the age of 6-8 weeks, which was still detectable when the second C. psittaci infection waves started. Animals on farm A were not vaccinated against APV leading to an APV subtype B outbreak accompanying the first C. psittaci infection wave. Despite subtype A APV vaccination on farms B and C, the second C. psittaci infection waves were accompanied (farm B) or followed (farm C) by a subtype B APV infection. On all farms respiratory signs always appeared together with a proven C. psittaci, APV and/or ORT infection. This study suggests an association between C. psittaci, APV and ORT, and indicates the multi-factorial aetiology of respiratory infections in commercial turkeys. All three pathogens should be considered when developing prevention strategies for respiratory disease.

  15. Clinical Analysis of Eight Cases of Pericardial Effusion Associated with Mycoplasma Pneumonia Infection in Children%儿童肺炎支原体感染相关心包积液八例临床分析

    Institute of Scientific and Technical Information of China (English)

    杨欣; 李熙鸿; 苏云洁; 李亚飞; 王静

    2015-01-01

    目的:探讨儿童肺炎支原体( MP)感染相关心包积液的临床特点,提高对该病的诊治水平。方法回顾2008年1月—2014年12月我院收治的儿童MP感染相关心包积液8例的临床资料,对临床特点、医技检查、治疗方法进行分析。结果8例均急性发病,有发热,咳嗽、气促4例,胸痛、胸闷3例,食欲缺乏1例。就诊初期6例诊断不明确,曾考虑心功能不全3例,川崎病2例,幼年特发性关节炎1例。8例心包积液均由超声心动图检查证实,其中2例因大量心包积液引起压迫症状行心包穿刺抽液处理。均经查血清MP-IgM抗体阳性明确诊断,全部予静脉-口服阿奇霉素序贯疗法治疗,6例痊愈出院,2例好转出院。出院后7例获随访,随访时间3~47个月,无一例复发。结论MP相关心包积液临床相对少见,易与其他导致心包积液的疾病相混淆而误诊,及时应用阿奇霉素疗效及预后均较好。%Objective To investigate the characteristics of pericardial effusion associated with mycoplasma pneumonia ( MP) infection in children. Methods Clinical data of eight cases with pericardial effusion associated with MP during Jan. 2008 and Dec. 2012, including clinical characteristics,accessory examinations and therapies,were collected and analyzed. Re-lated literature was reviewed. Results Eight patients ran a fever, and all the patients had a history of acute onset,four pa-tients had a cough and dyspnea, and three patients had stethalgia and chest stuffiness, and one patient had a poor appetite. Six patients were not clear in the early diagnosis. There were three patients once suspected with cardiac failure, two patients of Kawasaki disease and one patient of juvenile idiopathic arthritis. Eight patients with pericardial effusion were confirmed by ul-trasonic cardiogram. Pericardiocentesis and drainage were performed on 2 patients of children with pressure symptoms caused by quantity pericardial effusion. The serum

  16. Bioinformatic analysis of Chlamydophila pneumoniae Cpn0797 protein and the preparation of its monoclonal antibody%肺炎嗜衣原体Cpn0797蛋白的生物信息学分析及其单克隆抗体制备鉴定

    Institute of Scientific and Technical Information of China (English)

    陈超群; 吴移谋; 任林; 刘良专

    2012-01-01

    目的 生物信息学分析肺炎嗜衣原体Cpn0797蛋白的结构,制备特异性的抗Cpn0797蛋白的单克隆抗体.方法 用ProtParam、SignalP、NPS@、和PSORT等软件对Cpn0797蛋白的理化参数、信号肽、二级结构、蛋白亚细胞定位进行分析;原核表达纯化GST-Cpn0797融合蛋白,以其为免疫原免疫BALB/c小鼠.杂交瘤技术制备单克隆抗体,采用间接免疫荧光法鉴定单克隆抗体的亚类和特异性.结果 生物信息学分析表明Cpn0797蛋白二级结构以无规则卷曲为主;成功地建立了能稳定分泌抗Cpn0797蛋白的单克隆抗体杂交瘤细胞株,单克隆抗体能特异性的识别Cpn0797内源性蛋白.结论成功制备特异性的Cpn0797单克隆抗体,为进一步探究Cpn0797蛋白的生物学功能提供实验基础.%In order to analyze the protein structure of Chlamydophila pneumoniae Cpn0797 protein and to prepare spe cific monoclonal antibodies (mAb) against Cpn0797 protein, ProtParam, SignalP, NPS@ and PSORT software packages were used to predict the physical and chemical properties, signal peptide, secondary structure and protein localization sites in cells according to the amino acid sequence of Cpn0797. The gene cpn0797 was cloned into the prokaryotic expression vector pGEX6p 2, and expressed in E. Coli and then purified. The mAbs against Cpn0797 were prepared with the hybridoma tech nique after mice were immunized with purified GST Cpn0797 fusion protein. The isotype and specificities of the mAbs were de termined by indirect immunofluorescence assay (IFA). The bioinformatical analysis results showed that Cpn0797 protein main ly consisted of random coils. Hybridoma cell lines stably secreting mAbs against Cpn0797 protein are obtained. The mAbs re acted with Cpn0797 endogenous protein. In conclusion, the specific mAbs against Cpn0797 protein were obtained, which pro vides a basis for further study in the biological function of Cpn0797 protein.

  17. Relationship between the Cholesterol Level in Children with Asthma and Susceptibility of Mycoplasma Pneumoniae%哮喘患儿血清胆固醇水平与易感肺炎支原体的关系

    Institute of Scientific and Technical Information of China (English)

    张长娟; 段全纪; 周清霞

    2014-01-01

    目的:探讨支气管哮喘(简称哮喘)患儿血清胆固醇水平与易感肺炎支原体( MP )的关系。方法60例哮喘患儿分为哮喘组,40例健康儿童被分为正常对照组,检测两组血清总胆固醇水平,并进行1年的随访,记录随访期内肺炎支原体感染的次数。结果哮喘患儿血清总胆固醇水平及随访期内哮喘患儿肺炎支原体感染次数明显高于对照组,( P<0.05)。结论哮喘患儿存在血清胆固醇水平升高,其可致哮喘患儿更易感染肺炎支原体。%[ ABSTRACT] Objective To discuss the relationship between the cholesterol level in children with asthma and susceptibility of Mycoplasma pneumonia ( MP ) .Methods Sixty children with asthma were divided into the asthma group,and 40 healthy volunteers were divided into the healthy control group,then the cholesterol level was contrasted be-tween the two groups.Children with asthma and healthy volunteers were followed up for 1 year,and the number of MP in-fection during the follow-up period was recorded.Results The cholesterol level and the number of MP infection in the asthma group were significantly higher than those in the healthy control group.Conclusion High cholesterol exists in children with asthma and it can make children with asthma more vulnerable to infection MP.

  18. Evaluation of recombinant Mycoplasma hyopneumoniae P97/P102 paralogs formulated with selected adjuvants as vaccines against mycoplasmal pneumonia in pigs.

    Science.gov (United States)

    Woolley, Lauren K; Fell, Shayne A; Gonsalves, Jocelyn R; Raymond, Benjamin B A; Collins, Damian; Kuit, Tracey A; Walker, Mark J; Djordjevic, Steven P; Eamens, Graeme J; Jenkins, Cheryl

    2014-07-23

    Pig responses to recombinant subunit vaccines containing fragments of eight multifunctional adhesins of the Mycoplasma hyopneumoniae (Mhp) P97/P102 paralog family formulated with Alhydrogel(®) or Montanide™ Gel01 were compared with a commercial bacterin following experimental challenge. Pigs, vaccinated intramuscularly at 9, 12 and 15 weeks of age with either of the recombinant formulations (n=10 per group) or Suvaxyn(®) M. hyo (n=12), were challenged with Mhp strain Hillcrest at 17 weeks of age. Unvaccinated, challenged pigs (n=12) served as a control group. Coughing was assessed daily. Antigen-specific antibody responses were monitored by ELISA in serum and tracheobronchial lavage fluid (TBLF), while TBLF was also assayed for cytokine responses (ELISA) and bacterial load (qPCR). At slaughter, gross and histopathology of lungs were quantified and damage to epithelial cilia in the porcine trachea was evaluated by scanning electron microscopy. Suvaxyn(®) M. hyo administration induced significant serological responses against Mhp strain 232 whole cell lysates (wcl) and recombinant antigen F3P216, but not against the remaining vaccine subunit antigens. Alhydrogel(®) and Montanide™ Gel01-adjuvanted antigen induced significant antigen-specific IgG responses, with the latter adjuvant eliciting comparable Mhp strain 232 wcl specific IgG responses to Suvaxyn(®) M. hyo. No significant post-vaccination antigen-specific mucosal responses were detected with the recombinant vaccinates. Suvaxyn(®) M. hyo was superior in reducing clinical signs, lung lesion severity and bacterial load but the recombinant formulations offered comparable protection against cilial damage. Lower IL-1β, TNF-α and IL-6 responses after challenge were associated with reduced lung lesion severity in Suvaxyn(®) M. hyo vaccinates, while elevated pathology scores in recombinant vaccinates corresponded to cytokine levels that were similarly elevated as in unvaccinated pigs. This study highlights

  19. Development of Mycoplasma hyopneumoniae Recombinant Vaccines.

    Science.gov (United States)

    Marchioro, Silvana Beutinger; Simionatto, Simone; Dellagostin, Odir

    2016-01-01

    Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), a disease that affects swine production worldwide. Vaccination is the most cost-effective strategy for the control and prevention of the disease. Research using genome-based approach has the potential to elucidate the biology and pathogenesis of M. hyopneumoniae and contribute to the development of more effective vaccines. Here, we describe the protocol for developing M. hyopneumoniae recombinant vaccines using reverse vaccinology approaches.

  20. 婴幼儿支原体肺炎41例临床分析%Clinical Analysis of 41 Cases of Infant and Young Children with Mycoplasma Pneumonia

    Institute of Scientific and Technical Information of China (English)

    徐金生

    2015-01-01

    目的:根据小儿肺炎支原体感染的临床表现和特征,探讨对其诊断与治疗的方法。方法:选取笔者所在医院2011年4月-2014年4月收治的41例肺炎支原体感染患儿作为研究对象,对其临床资料进行回顾性分析。41例均有不同程度的咳嗽、咳痰;其中29例患者有发热病史,34例患者就诊时仅表现为肺部呼吸音减弱或增粗、无干湿啰音。一周后出现湿啰音,伴喘鸣音有9例,呼吸音减低有1例,轻度胸痛5例,病变区叩诊浊音3例,无阳性体征者4例。肺外表现:窦性心动过速4例,窦性心律不齐2例,S-T段及T波改变2例。感染性贫血1例,恶心、呕吐13例,腹痛8例,肝功能异常6例,皮疹2例,部分患者伴有胃肠道症状及局部表浅淋巴结肿大。X线检查:胸片提示斑片状或点状阴影,伴胸膜反应3例。心电图检查:33例正常,4例提示窦性心动过速,S-T段及T波改变2例,窦性心律不齐2例。结果:经过药物治疗,41例肺炎支原体感染患儿均临床治愈。结论:婴幼儿支原体肺炎临床表现不典型,肺外表现多,经合理治疗预后良好。%Objective:According to the clinical manifestations of the pediatric pneumonia mycoplasma infection and characteristics,to discuss the methods of diagnosis and treatment.Method:41 children with mycoplasma pneumoniae infection in our hospital from April 2011 to April 2014 were selected as the research objects,the clinical data were retrospectively analyzed.41 patients had different degree of cough,sputum.Including 29 cases with a history of fever,34 cases showed only a diminished or enlargement of lung breath sounds,no dry wet rale when seeing a doctor.Wet rale appeared after a week,9 cases with wheezing,decreased breath sounds in 1 case,mild chest pain in 5 cases,the lesion area percussion dullness in 3 cases, no positive signs in 4 cases.Extrapulmonary manifestations:4 cases were sinus

  1. Effect observation of pidotimod oral liquid treating Mycoplasma pneumo-nia in children%匹多莫德口服液治疗小儿支原体肺炎的效果观察

    Institute of Scientific and Technical Information of China (English)

    闫东梅

    2015-01-01

    目的:探讨匹多莫德口服液治疗小儿支原体肺炎的临床效果。方法回顾性分析本院2010年1月~2014年1月收治的支原体肺炎患儿200例的临床资料。根据治疗方法不同将患者分为两组,两组均予退热、止咳、化痰处理,同时配以吸氧、纠正水电解质紊乱和维持酸碱平衡等常规治疗,对照组在此基础上采用阿奇霉素治疗,观察组在常规治疗基础上予匹多莫德治疗。比较两组的相关指标和临床疗效。结果观察组的肺部啰音消失时间为(96±15)h,肺部炎症吸收时间为(79±21)h,对照组分别为(168±18)、(164±24)h,观察组的肺部啰音消失时间、肺部炎症吸收时间分别低于对照组,差异有统计学意义(P<0.05);观察组的总有效率(95.0%)高于对照组(78.0%),差异有统计学意义(P<0.05);观察组治疗后的血清IgA、IgG、IgM水平与对照组比较,差异有统计学意义(P<0.05);观察组治疗后的血清CD3+、CD4+、CD8+、CD4+/CD8+水平与对照组比较,差异有统计学意义(P<0.05)。结论匹多莫德口服液治疗小儿支原体肺炎效果显著,值得在儿科推广。%Objective To investigate the clinical effect of pidotimod oral liquid treating Mycoplasma pneumonia in children. Methods The clinical data of 200 children with Mycoplasma pneumonia treated in our hospital from January 2010 to January 2014were analyzed retrospectively.According to the different treatment methods,they were divided into the two groups,the two groups were given the conventional treatment,such as abatement of fever,relieving cough, reduc-ing phlegm,at the same time,they were treated with oxygen uptake,correcting water and electrolyte disorders,maintaining acid-base balance and so on,the control group was treated with azithromycin,the observation group was given pidotimod on the basis of the conventional treatment.The related indictors and curative effect in the two groups were compared. Results

  2. 异功散合玉屏风散加减治疗小儿肺炎支原体肺炎后期脾虚证疗效观察%Yigong powder with Yupingfeng powder for syndrome of spleen deficiency in children with mycoplasma pneumoniae pneumonia

    Institute of Scientific and Technical Information of China (English)

    朱树丽; 施益农

    2015-01-01

    ObjectiveTo evaluate the clinical efficacy ofYigongpowder withYupingfengpowder for syndrome of spleen deficiency in children with mycoplasma pneumoniae pneumonia.MethodsA total of 60 patients who had spleen deficiency after azithromycin injection 5 days were randomly divided into treatment group and control group according to the random number table, with 30 in each group. The patients in both received azithromycin suspension for 2 weeks, while the patients in the treatment group received azithromycin suspension and traditional Chinese medicineYigongpowder withYupingfengpowder for 2 weeks. The symptoms score was used to evaluate the clinical efficacy.ResultsThe scores of appetite loss (0.65 ± 1.00vs. 2.32 ± 1.28,t=5.631), abdominal distention (0.73 ± 0.23vs.1.33 ± 0.97,t=3.297) and fatigue (0.72 ± 0.32vs. 1.42 ± 1.18,t=3.136) in the treatment group were significantly lower than those in the control group (all P<0.05). The total effective rate for syndrome of spleen deficiency in the treatment group was significantly higher than thatin the control group(90.0%vs.66.7%;χ2=4.812,P<0.05).ConclusionYigongpowder withYupingfeng powder can significantly improve the symptoms of syndrome of spleen deficiency in children with mycoplasma pneumoniae pneumonia.%目的:评价异功散合玉屏风散加减治疗小儿肺炎支原体肺炎后期脾虚证的临床疗效。方法本研究为随机对照研究。将符合纳入标准静滴5 d阿奇霉素后出现脾虚证的60例患儿,按随机数字表法分为2组各30例。对照组继续口服阿奇霉素2周,治疗组在对照组基础上加用异功散合玉屏风散2周,观察症状积分改善情况判定疗效。结果治疗组食欲不振[(0.65±1.00)分比(2.32±1.28)分,t=5.631]、食后腹胀[(0.73±0.23)分比(1.33±0.97)分,t=3.297]、神疲懒言[(0.72±0.32)分比(1.42±1.18)分,t=3.136]积分均较对照组降低(P均<0.05)。治疗组肺炎支原体肺炎后期脾

  3. Application value of serum TNF-α, IL-6, IL-8, IL-10 in children mycoplasma pneumoniae pneumonia%血清TNF-α、IL-6、IL-8、IL-10在儿童肺炎支原体肺炎中的应用价值

    Institute of Scientific and Technical Information of China (English)

    李锦; 郭瑞雪; 王金虎

    2015-01-01

    目的:对血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和白细胞介素-10(IL-10)浓度在儿童肺炎支原体肺炎(MPP)中的应用价值进行探讨。方法90例肺炎支原体肺炎患儿作为肺炎组,其中轻症组50例,重症组40例;100例健康体检儿童作为对照组,对比肺炎组与对照组血清TNF-α、IL-6、IL-8和IL-10指标及轻症组与重症组血清中的各因子浓度。结果对照组与肺炎组治疗前血清中TNF-α、IL-6、IL-8和IL-10浓度差异具有统计学意义(P0.05), TNF-α、IL-6及IL-8浓度均显著低于重症组,差异具有统计学意义(P0.05). They had much lower TNF-α, IL-6, IL-8 than the severe group, and their differences had statistical significance (P<0.05).Conclusion Serum TNF-α, IL-6, IL-8, IL-10 have certain correlation with mycoplasma pneumoniae pneumonia, and they are closely correlated with MPP degree. They can be used as evaluation indexes for clinical diagnosis, treatment and prognosis.

  4. [Community acquired pneumonia in children: an update for outpatients management].

    Science.gov (United States)

    Wagner, Noémie; Gehri, Mario; Gervaix, Alain; Guinan, Stéphane; Barazzone-Argiroffo, Constance

    2016-02-17

    Pneumonia should be considered in febrile children with tachypnea and/or chest recession. Virus are the most common cause of pneumonia in children under 5 years old. Streptococcus pneumonia can be found at any age. Mycoplasma pneumonia is more frequent in older children. Systematic chest radiograph is not necessary but must be obtained in patients with hypoventilation and in those with failed initial antibiotic therapy. Mycoplasma pneumonia should be tested according to patient age and response to initial antibiotic. First line antibiotherapy is amoxicilline. Antibiotic treatment is frequently not necessary in children under 5 but should be considered depending on clinical presentation and C reactive protein value.

  5. Pneumonia bacteriana adquirida na comunidade

    OpenAIRE

    Machado, Lais Del Prá Netto

    2015-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Farmácia, Florianópolis, 2015. A pneumonia pode ser causada por diversos microrganismos e classificada de forma abrangente, havendo poucos e frágeis estudos clínicos e epidemiológicos sobre pneumonias adquiridas na comunidade (PACs). Os patógenos mais frequentes nas PACs são Streptococcus pneumoniae e Haemophilus influenzae (em pneumonias típicas) e Mycoplasma pneumoni...

  6. Regulation of gene expression in Mycoplasmas: contribution from Mycoplasma hyopneumoniae and Mycoplasma synoviae genome sequences

    Directory of Open Access Journals (Sweden)

    Humberto Maciel França Madeira

    2007-01-01

    Full Text Available This report describes the transcription apparatus of Mycoplasma hyopneumoniae (strains J and 7448 and Mycoplasma synoviae, using a comparative genomics approach to summarize the main features related to transcription and control of gene expression in mycoplasmas. Most of the transcription-related genes present in the three strains are well conserved among mycoplasmas. Some unique aspects of transcription in mycoplasmas and the scarcity of regulatory proteins in mycoplasma genomes are discussed.

  7. Clinical Research in Kawasaki Disease with Mycoplasma Pneumoniae Infection in Children%川崎病合并肺炎支原体感染患儿的临床研究

    Institute of Scientific and Technical Information of China (English)

    周益平; 符辰璐

    2016-01-01

    Objective To analyze clinical characteristics and laboratory data of children with mycoplasma pneumoniae (MP) infection of Kawasaki disease (KD).MethodsTotally 62 KD children with MP infection who were hospitalized from January 2010 to January 2016 in the First People's Hospital of Changzhou were divided into KD with MP group (18 children) as the MP group, and KD without MP group (44 children) as the control group. The blood routine, blood sedimentation, CRP and thermal were determined.Results The neutrophilic granulocyte count, leucocyte count, hematocrit, platelet count and erythrocyte sedimentation rate showed no difference between MP group and control group (P>0.05). CRP and thermal process showed significant difference between MP group and control group (P<0.05). Also the coronary artery expansion (coronary artery dilated, CAD) was signiifcantly different between the two groups (P<0.05). In the course of 5 to 10 days, both groups were given intravenous immunoglobulin (IVIG) 1 g/(kg•d) for 2 days, and aspirin 50 mg/(kg•d) (oral administration of three times). Then the MP group was given azithromycin for the sequential therapy.Conclusion MP infection may be one of the causes of KD. MP infection can induce or worsen the condition of KD children, and increase the risk of coronary artery expansion, ifnally would prolong the course and affect the prognosis.%目的:探讨川崎病(Kawasaki disease, KD)患儿合并肺炎支原体(mycoplasma pneumoniae,MP)感染患儿的临床特征。方法回顾性分析于2010年1月—2016年1月收治常州市第一人民医院儿科临床诊断为KD的62例患儿,其中合并MP感染的18例为MP组,其余44例为对照组。比较两组血常规、血沉、C反应蛋白(CRP)、热程等指标。结果 MP组与对照组患儿血白细胞总数、中性粒细胞计数、红细胞压积、血小板计数及血沉差异均没有统计学意义(P>0.05)。MP组热程较对照组长,CRP高于对照组

  8. PREVALENCE OF MYCOPLASMA CAPRICOLUM SUBSPECIES CAPRICOLUM AND MYCOPLASMA PUTREFACIENS IN GOATS IN PISHIN DISTRICT OF BALOCHISTAN

    Directory of Open Access Journals (Sweden)

    M. A. AWAN, F. ABBAS, M. YASINZAI1, R. A. J. NICHOLAS2, S. BABAR, R. D. AYLING2, M. A. ATTIQUE AND Z. AHMED

    2009-10-01

    Full Text Available Several Mycoplasma species cause serious and economically important diseases in goats world-wide. Forty goat flocks in the Pishin district of Balochistan province of Pakistan were examined for the clinical cases of contagious caprine pleuropneumonia (CCPP during 2008. Thirty goats suspected for CCPP on the basis of respiratory symptoms were euthanized for post mortem examination, microbiological and molecular studies. Two types of Mycoplasma species were isolated and identified by biochemical and growth inhibition (GI tests and polymerase chain reaction (PCR. Mycoplasma capricolum subspecies capricolum was isolated from 12(40% of the nasal swabs and 12(40% of the respective lung cultures, whereas 2(6.7% Mycoplasma putrefaciens (Mp isolates from nasal swab and lung cultures and 1(3.3% from liver and intestine cultures were recovered. We report probably for the very first time the isolation and identification of Mycoplasma capricolum subspecies capricolum (Mcc and Mp from the nasal swabs and lungs of goats with respiratory problems in Pishin district of Balochistan. Experimental studies to reproduce pneumonia or pleuropneumonia by Mcc and Mp organisms in susceptible goats or other laboratory animal models are further needed.

  9. Secretomes of Mycoplasma hyopneumoniae and Mycoplasma flocculare reveal differences associated to pathogenesis.

    Science.gov (United States)

    Paes, Jéssica A; Lorenzatto, Karina R; de Moraes, Sofia N; Moura, Hercules; Barr, John R; Ferreira, Henrique B

    2017-02-10

    Mycoplasma hyopneumoniae and Mycoplasma flocculare cohabit the porcine respiratory tract. However, M. hyopneumoniae causes the porcine enzootic pneumonia, while M. flocculare is a commensal bacterium. Comparative analyses demonstrated high similarity between these species, which includes the sharing of all predicted virulence factors. Nevertheless, studies related to soluble secretomes of mycoplasmas were little known, although they are important for bacterial-host interactions. The aim of this study was to perform a comparative analysis between the soluble secreted proteins repertoires of the pathogenic Mycoplasma hyopneumoniae and its closely related commensal Mycoplasma flocculare. For that, bacteria were cultured in medium with reduced serum concentration and secreted proteins were identified by a LC-MS/MS proteomics approach. Altogether, 62 and 26 proteins were identified as secreted by M. hyopneumoniae and M. flocculare, respectively, being just seven proteins shared between these bacteria. In M. hyopneumoniae secretome, 15 proteins described as virulence factors were found; while four putative virulence factors were identified in M. flocculare secretome. For the first time, clear differences related to virulence were found between these species, helping to elucidate the pathogenic nature of M. hyopneumoniae to swine hosts.

  10. 猪Toll样受体基因的变异及其与支原体肺炎感染的关系%Genetic Variation of Toll-like Receptors and Mycoplasma pneumoniae Infection in Pigs

    Institute of Scientific and Technical Information of China (English)

    方晓敏; 刘筱; 孟翠; 任守文; 李碧侠; 王学敏; 涂枫

    2012-01-01

    As significant pattern-recognition receptors,Toll-like receptors(TLRs) play an important role in the innate immunity and serve as the first barrier in the host defense against microbial pathogens. Variation of TLR genes would affect the resistance or susceptibility to diseases in human beings and animals. The purpose of this article is to analyze the correlation between the genetic variation of Toll-like receptors and Mycoplasma pneumoniae Infection in pigs. In this study .PCR-SSCP was applied to detect the distribution of single nucleotide polymorphisms (SNPs)in Western Commercial pig breeds( Landrace,Large White,Pietrain) .Chinese indigenous pig breeds (Meis-han,Erhualian,Jiangquhai,Jinhua)and Jiangsu cultivated breed( Suzhong pig). And then porcine alveolar tnacro-phages( PAMs)of different genotype were stimulated by LPS and the expression levels of TLR2 ,TLR4 and pro-inflammatory cytokines TNF-a,IL-l|3 at different time points were detected by real-time PCR to reveal the relationship between mutations in porcine TLRs and Mycoplasma pneumonia infection. Results show that: ( 1) Overall three non-synonymous SNPs(T611A,G962A,C1027A)and a synonymous SNPs( G960A)were found in TLR4 coding sequence, and the distribution of C1027A was biased in Western pig and Chinese indigenous pig populations. (2)The expression of TLR2, TLR4 and TNF-a, IL-1 £ were enhanced at different levels by LPS stimulation. TLR2, TLR4 gene expression and growth rate of CC-genotype in 1 027 bp of TLR4 were significantly higher than AC-genotypepigs(P<0.01) ,whereas TNF-a,IL-lp expression was significantly lower than that of AC groups(P <0. 01). Thus, alleles C in 1 027 bp(C 1 027 A)of TLR4 may probably be the resistance gene to M. Pneumonia infections in pigs.%Toll样受体(TLRs)作为重要的模式识别受体,在动物的天然免疫中发挥重要作用,是机体抵抗感染的第一道屏障;TLRs基因变异会改变机体对病原的抗性或易感性,本试验旨在探讨TLR2、TLR4

  11. Respi ratory tract infection pneumonia mycoplasma survey and its correlation with seasonal factor%呼吸道感染肺炎支原体调查与季节因素的相关性研究

    Institute of Scientific and Technical Information of China (English)

    史云菊; 柳璐; 李肖静; 郭红霞; 张秋君

    2016-01-01

    目的:探讨呼吸道感染肺炎支原体与季节因素的相关性,了解季节对呼吸道感染的影响。方法收集2012年1月-2015年1月就诊的2323例呼吸道感染临床资料,分析甲型流感病毒(IN FA )、副流感病毒(PIV )、乙型流感病毒(INFB)、呼吸道合胞病毒(RSV)、腺病毒(ADV)、肺炎支原体(MP)、肺炎衣原体(CP)、嗜肺军团菌(LP)8种病原体血清IgM检测结果。结果有1253例IgM 阳性,阳性率53.94%,其中INFA、INFB、MP、LP、PIV、ADV、CP、RSV阳性率分别为17.78%、13.00%、9.34%、5.81%、3.53%、2.20%、1.42%、0.86%;男性,18~60岁、下呼吸道感染患者M P‐Ig M阳性率分别为10.52%、14.07%、11.43%,高于女性,>60~80岁、>80岁、上呼吸道感染患者M P‐Ig M阳性率,比较差异有统计学意义;冬季、春季、秋季及夏季M P‐Ig M阳性率分别为12.03%、10.81%、6.79%、4.98%,其中M P‐IgM阳性率冬季、春季均高于秋季、夏季,比较差异无统计学意义;Spe‐raman相关分析研究年度内气温与患者MP‐IgM阳性率间有负相关性(P<0.05)。结论成人呼吸道感染病原体中MP感染较为常见,男性、中青年人及下呼吸道感染患者MP感染率相对较高,气温与MP感染关系密切,夏秋季气温较高的季节MP感染率相对低于冬春季气温较低季节,低气温可能是影响MP流行的主要气象学因素。%OBJECTIVE To discuss respiratory tract mycoplasma pneumoniae infection and its correlation with sea‐sonal factor ,and understand the influence of the season for respiratory tract infections .METHODS Clinical data of 2323 patients with respiratory tract infections in hospital from Jan .2012 to Jan .2012 were collected .The serum IgM antibody test results of eight kinds of pathogens were analyzed ,including influenza a virus (INFA ) ,parain

  12. Efficacy of combination therapy of mycoplasma pneumonia: a Meta analysis%中西医结合治疗小儿肺炎支原体肺炎效果的Meta分析

    Institute of Scientific and Technical Information of China (English)

    黄玉瑛; 邱钟燕; 朱思刚

    2014-01-01

    Objective To discuss the clinical efficacy of Chinese and Western medicine inmycoplasma pneumonia in children.Methods Randomized controlled trials (RCTs) on western medicine versus traditional Chinese combined with Western medicine treating children mycoplasma pneumonia were identified according to the inclusion and exclusion criteria.Methodological quality of included studies was accessed.Meta-analysis was then performed using RevMan 4.2 software.Pooled odds ratio (OR),weighted mean difference (WMD) and 95% confidence interval (CI) were calculated.20 randomized controlled domestic researches were gathered.Results Cure rate and effective rate in the experimental group were better than those in the control group(OR =2.92,95% CI:2.29 to 3.74,P <0.01).Inefficiency in the experimental group was lower than in control group; the difference was statistically significant (OR =0.20,95% CI:0.12 to 0.34,P < 0.01) ; rale disappearance time,cough disappearance time and cooling time in the experimental group were shorter than those in control group(WMD =-2.22,95% CI:-2.43 to-2.01,P<0.01),(WMD =-1.61,95% CI:-2.35 to-0.86,P<0.01),(OR=-0.90,95% CI:-1.43 to-0.37,P =0.0008).The incidence of adverse reactions in the experimental group was lower than that in the control group; the difference was statistically significant,(WMD =0.16,95% CI:0.05 to 0.48,P =0.001).Conclusion Treatment of mycoplasma pneumonia in children with Chinese and Western Medicine can significantly increase the efficiency of recovery; the non-efficiency and the incidence of adverse reactions can be significantly reduced.%目的 评价中西医结合治疗小儿肺炎支原体肺炎的临床效果.方法 收集国内关于中西医结合治疗和单纯西医治疗小儿肺炎支原体肺炎的随机对照研究文献,对结果进行Meta分析.其中,试验组进行中西医结合治疗,在应用抗生素的基础上服用各式中药煎剂;对照组进行抗生素治疗.疗效及差异评价指

  13. The clinical study on the combined detection of mycoplasma pneumoniae and chlamydia pneumoniae of the patients with community acquired pneumonia%社区获得性肺炎患者联合检测肺炎支原体和衣原体的临床研究

    Institute of Scientific and Technical Information of China (English)

    陈雪莹; 张卫星

    2014-01-01

    目的:观察分析联合检测肺炎支原体(Mp)和肺炎衣原体(Cp)对社区获得性肺炎(CAP)患者的临床意义。方法选取1500例 CAP 患者作为研究对象,对其咽拭子标本中的 Mp 和 Cp DNA 进行检测,对主要病原菌进行培养和鉴定,观察比较患者的抗感染治疗方案和治愈率。结果本组 CAP 患者的病原体分布以 Mp、肺炎链球菌、流感嗜血杆菌和 Cp 的比例最大,而感染类型以2种病原体感染的比例最多;在本组患者的治疗过程中,β-内酰胺类抗菌药物的使用频率最高,应用β-内酰胺类联合大环内酯类或氟喹诺酮类药物治疗的患者的治愈率显著高于单独应用β-内酰胺类药物的患者(P <0.05);在治疗Mp 或 Cp 单纯感染时,单独应用β-内酰胺类药物与应用β-内酰胺类联合大环内酯类或氟喹诺酮类药物治疗的患者治愈率无显著性差异(P >0.05);在治疗细菌合并 Mp 或 Cp 感染时,应用β-内酰胺类联合大环内酯类或氟喹诺酮类药物的患者治愈率显著高于单独应用β-内酰胺类药物者(P <0.05)。结论 Mp 和 Cp 感染在 CAP 患者中比较多见,患者的主要感染类型是2种病原体的混合性感染,β-内酰胺类药物是治疗过程中常用药物,应用β-内酰胺类联合大环内酯类或氟喹诺酮类药物的抗感染方案可提高患者的治愈率,特别是在有细菌合并 Mp 或 Cp 感染时,可发挥显著疗效,临床医生应根据 Mp 或 Cp 的检测结果合理、有效地选择抗感染治疗方案。%Objective To observe and analyze the clinical significance of the combined de-tection of Mycoplasma pneumoniae (Mp)and Chlamydia pneumoniae (Cp)for the patients with community acquired pneumonia (CAP).Methods 1500 CAP patients were selected as the re-search objects.DNA of Mp and Cp in the throat swab specimens of the patients were detected and the main pathogenic bacteria were

  14. 肺炎支原体OsmC蛋白的原核表达及活性分析%Prokaryotic expression, purification, and determination of the peroxidase activity of the OsmC protein from Mycoplasma pneumoniae

    Institute of Scientific and Technical Information of China (English)

    唐愈菲; 朱翠明; 黄泽智; 赵爽

    2016-01-01

    目的 表达肺炎支原体(Mycoplasma pneumoniae)渗透压诱导蛋白C(OsmC)并测定其活性. 方法 在Mp中找出编码OsmC蛋白的基因,根据Mp OsmC基因序列设计特定引物,PCR扩增OsmC基因.利用EcoRI和XhoI对其进行双酶切,然后连接到表达载体pET28a,构建重组质粒pET28a MpOsmC,转化大肠埃希菌BL21 (DE3),IPTG诱导目的蛋白表达,采用镍柱亲和层析对重组OsmC蛋白进行纯化,利用氧化铁二甲酚橙试剂(FOX)测定OsmC蛋白的活性. 结果 肺炎支原体Mpn625基因编码OsmC蛋白.PCR扩增OsmC基因片段大小为426 bp,连接到原核表达载体pET28a得到重组质粒pET28a MpOsmC.重组质粒转化大肠埃希菌BL21(DE3),IPTG诱导表达分子质量单位为19.4 ku的重组Mp OsmC蛋白,与理论值相符.通过亲和层析,得到高纯度的目的蛋白.FOX试剂法测定Mp OsmC具有分解H2O2活性. 结论 重组Mp OsmC蛋白具有氧化酶活性,为探讨肺炎支原体的抗氧化机制提供了理论依据.

  15. Study on the relationship between bronchial asthma in children and mycoplasma pneumonia(MP)infection%肺炎支原体感染和小儿支哮喘的关系研究

    Institute of Scientific and Technical Information of China (English)

    李晓燕; 邵学津; 柴晓文

    2011-01-01

    目的:探讨小儿支气管哮喘与肺炎支原体(MP)感染的关系.方法:儿童哮喘专科门诊及住院哮喘发作期患儿386例(哮喘组)及同期就诊的呼吸道感染患儿426例(对照组),采用MP被动冷凝集法检测血清肺炎支原体抗体(MP-Ab).结果:哮喘组MP-Ab阳性186例,阳性率48.2%,对照组阳性106例,阳性率25.8%a,两组比较差异显著(P<0.01).结论:MP感染与儿童哮喘关系密切,MP导致呼吸道慢性炎症,进而改变呼吸道高反应性是最根本的机制.对哮喘发作期的患儿要考虑MP感染可能,应常规行MP-Ab检查.%Objective:To discuss the relationship between bronchial asthma in children and mycoplasma pneumonia(MP) infection. Methods: All the 386 cases of paroxysmal asthma( bronchial asthma group) from our hospital were selected. 426 children with respiratory tract infection served as control group. MP- Ab was detected by CAT method in both groups. Results: MP- Ab was postive in 186cases (48.2%)in bronchial asthma group; 106 cases (25.8%) in control group. There was a significant difference between the two groups (P < 0.01 ). Conclusion: There is a close relationship between MP infection and children bronchial asthma. The estivity of respiratory tract. The possibility of MP infection should be taken into account while treating children with paroxysmal asthma. MP - Ab should be detected routinely in children with paroxysmal asthma.

  16. Epizootic abortion related to infections by Chlamydophila abortus and Chlamydophila pecorum in water buffalo (Bubalus bubalis).

    Science.gov (United States)

    Greco, G; Corrente, M; Buonavoglia, D; Campanile, G; Di Palo, R; Martella, V; Bellacicco, A L; D'Abramo, M; Buonavoglia, C

    2008-06-01

    Water buffalo (Bubalus bubalis) are affected by high rates of embryonic mortality and abortion related to infectious diseases and non-infectious factors. A number of viral and bacterial infections have been associated with reproductive failure, but there is limited information on the role of chlamydial infections. In order to investigate the presence and the role of Chlamydiaceae in water buffalo a retrospective study was performed in a herd with a history of reproductive failure. During an 11-month period, the pregnant heifers suffered an abortion rate of 36.8% between the 3rd and 7th month of pregnancy. Antibodies to Chlamydiaceae were detected in 57% of the aborted cows, and in 0% of the overtly healthy cows used as control. By a nested-PCR assay, three of 14 vaginal swabs from aborted animals tested positive for Chlamydophila agents and, additionally, three out of seven aborted fetuses tested positive for Chlamydophila spp., with two being co-infections by Cp. abortus and Cp. pecorum and one being characterised as Cp. abortus. Sequence analysis of the amplicons confirmed the results of the nested-PCR. The presence of anti-Chlamydiaceae antibodies in more than half of the aborting animals (PChlamydophila agents in several fetal organs and in the vaginal swabs are consistent with the history of abortions observed in the herd and suggest an abortifacient role by Chlamydophila spp. in water buffalo (B. Bubalis) herds.

  17. Research on Infantile Pneumonia Mycoplasma Infection and Bacterial Infection of the Blood Routine and the Changes of C-Reactive Protein%肺炎支原体感染及细菌感染患儿部分血常规指标及C反应蛋白水平

    Institute of Scientific and Technical Information of China (English)

    古丽比亚·卡合曼; 林磊

    2016-01-01

    Objective:To compare pediatric pneumonia mycoplasma infection and bacterial infection of the blood routine and the change pattern of c-reactive protein. Methods:160 children with myco-plasma pneumoniae infection and bacterial infection in equal numbers,compared to 80 normal. Testing blood cells,central neutrophils,lymphocytes and mononuclear cell count,and compare three groups of children with red blood cells,hemoglobin. ELISA was adopted to test the C-reactive protein level of three groups. Results:Comparing RBC and PLT counting,difference was not statistically significant (P>0. 05);children with mycoplasma pneumoniae infection of anaemia,the decrement of platelet and leukocyte loss were significantly higher than the control group and bacterial infection group( P0 . 05 );in children with mycoplasma infection group of hemoglobin concentration was lower than that in group a bacterial infection(P0. 05). Conclusion:children with mycoplasma pneumoniae infection and children with bacterial infection could be distinguished by WBC counting,Hb content and CRP level.%目的:比较肺炎支原体感染或细菌感染患儿的血常规及C 反应蛋白( CRP)的变化规律。方法:肺炎支原体感染(支原体感染组)与细菌感染(细菌感染组)患儿各80例,80例正常儿童作为对照组,检测入院或体检时3组儿童血红蛋白( Hb)含量、白细胞( WBC)和红细胞( RBC)及血小板( PLT)计数,中性粒细胞、淋巴细胞及单核细胞分类;采用酶联免疫法( ELISA)法测定3组儿童血清中C反应蛋白( CRP)水平。结果:3组儿童RBC及PLT计数比较,差异无统计学意义( P>0.05);与对照组比较,细菌感染组和支原体感染组WBC计数、中性粒细胞和单核细胞分类及血清CRP水平升高,淋巴细胞分类降低,而Hb含量仅支原体感染组降低,单核细胞分类仅支原体感染组升高,差异有统计学意义( P<0.05);与细菌感

  18. Chlamydophila felis in cats--are the stray cats dangerous source of infection?

    Science.gov (United States)

    Halánová, M; Sulinová, Z; Cisláková, L; Trbolová, A; Páleník, L; Weissová, T; Halán, M; Kalinová, Z; Holičková, M

    2011-11-01

    Chlamydophila felis is a causative agent of acute or chronic conjunctivitis, and pneumonia in cats. Natural transmission mostly occurs consequently to close contact with other infected cats, their aerosol and fomites. We have examined 93 cats with symptoms of acute or chronic conjunctivitis, from Košice region in Slovakia, during the period of 2 years. Conjunctival samples were obtained from 55 domestic cats (59.14%) and 38 stray cats (40.86%). Of the total number of 93 examined animals, 42 cats were positive, which represents 45.16% overall positivity. Out of the 42 positive cats, 25 cats were stray and 17 positive cats were classified as domestic, which means that of 38 stray cats, 25 were positive (which represented 65.78% positivity) and of 55 domestic cats, 17 were positive (positivity was 30.90%). Our results showed that cats, especially stray cats, could be a dangerous source of chlamydiosis for humans.

  19. Secretion of TNF-α induced by heat shock protein 10 of Chlamydophila pneumoniae were mediated by TLR2 and TLR4 in THP-1%肺炎嗜衣原体热休克蛋白10经TLR2及TLR4调控THP-1分泌TNF-α

    Institute of Scientific and Technical Information of China (English)

    周洲; 杨科; 陈丽丽; 陈虹亮; 李忠玉; 吴移谋

    2012-01-01

    目的 探讨肺炎嗜衣原体(Cpn)热休克蛋白10(HSP10)诱导人单核细胞分泌炎症因子的作用及Toll样受体(TLR)2、TLR4与此作用的相关性.方法 制备Cpn HSP10(CHSP10)纯化蛋白,去内毒素活性后用不同浓度(0.5、1、5、10、20、30μ/ml)刺激THP-1细胞0、6、12、24、36、48、60 h,并比较蛋白不同处理组别中TNF-α的水平差异;以间接免疫荧光及RT-PCR鉴定THP-1细胞上的TLR4及TLR2;分离C3H系野生型和TLR4缺陷型小鼠巨噬细胞,以CHSP10刺激后检测TNF-α水平;用抗TLR2/TLR4单克隆抗体预孵育细胞,ELISA检测CHSP10刺激细胞前后TNF-α的变化.结果 CHSP10刺激THP-1细胞引起上清液炎症因子TNF-α水平显著增加,经加热等处理后蛋白诱生TNF-α的作用明显降低.THP-1细胞可检测到TLR2及TLR4的mRNA及蛋白表达,CHSP10诱生C3H系野生型小鼠细胞分泌的TNF-α明显高于TLR4缺陷型小鼠细胞,TLR2/4经单克隆抗体作用后均可显著降低CHSP10诱导THP-1分泌TNF-α的水平.结论 CHSP10可能作为炎症相关蛋白参与了Cpn对宿主细胞的致炎作用;并且TLR2及TLR4在该炎症刺激信号的传递过程中发挥一定的作用.%Objective To investigate the effect of heat shock protein 10 (HSP1O) of Chlamydophila pneumoniae in inducing TNF-α on THP-1 cells and the roles of TLR4 and TLR2 involved in it.Methods Purified native recombinant HSP10 from Cpn(CHSP10) were produced and inactivated the endotoxin contamination,then different concentration (0.5,1,5,10,20,30 μg/ml) of CHSP10 were used to stimulate THP-1 for different time (0,6,12,24,36,48,60 h).TNF-α were measured by using human TNF-α ELISA kit and compared among different groups.THP-1 were collected and analyzed for TLR2 and TLR4 mRNA levels and protein expression by RT-PCR and immunofluorescence.Peritoneal macrophages isolated from wide-type (C3 H/HeN) and TLR4-deficient mice (C3H/HeJ) were stimulated with endotoxin-free proteins respectively,and the TNF

  20. Molecular biology of Mycoplasma

    DEFF Research Database (Denmark)

    Christiansen, Gunna; Jensen, Lise T.; Boesen, Thomas

    1997-01-01

    Mycoplasmas are the smallest free living microorganisms with the smallest genome. The G+C content is in general low (25-33%) and the coding capacity is about 600 proteins. Mycoplasma species are phylogenetically related, they use the genetic codon UGA for tryptophan, and show rapid evolution, wit...

  1. Molecular biology of Mycoplasma

    DEFF Research Database (Denmark)

    Christiansen, Gunna; Jensen, Lise T.; Boesen, Thomas

    1997-01-01

    Mycoplasmas are the smallest free living microorganisms with the smallest genome. The G+C content is in general low (25-33%) and the coding capacity is about 600 proteins. Mycoplasma species are phylogenetically related, they use the genetic codon UGA for tryptophan, and show rapid evolution...

  2. Surgical infections with Mycoplasma

    DEFF Research Database (Denmark)

    Levi-Mazloum, Niels Donald; Prag, Jørgen Brorson; Jensen, J S

    1997-01-01

    Mycoplasma hominis and Ureaplasma urealyticum are common inhabitants of the human genital tract. Evidence for an aetiological role in pyelonephritis, pelvic inflammatory disease, post-abortion and post-partum fever has been presented. There are sporadic reports of Mycoplasma causing serious...

  3. Analysis on Mycoplasma pneumonia 23SrRNA gene mutation site and drug resistance phenotype%肺炎支原体23SrRNA基因突变位点与耐药表型的分析

    Institute of Scientific and Technical Information of China (English)

    叶芸; 李苏亮; 姜萍; 王瑶; 杨超

    2013-01-01

    Objective To investigate the infection situation of Mycoplasma pneumonia ( Mp ) in patients with community-acquired respiratory tract infection and the molecular drug resistance mechanisms of macrolide,and to analyze the relationship between 23SrRNA gene mutation site of isolates resistant to Mp and drug resistance phenotype. Methods A total of 400 throat swab specimens of community-acquired respiratory tract infection were cultured to isolate Mp,the clinical isolates were identified by nested polymerase chain reaction, and the in vitro antibiotic sensitivity test was performed for identifying macrolide-resistant isolates through the minimal inhibitory concentration( MIC).The sequences of macrolide-resistant 23SrRNA gene were detected. The sequences were compared to the corresponding sequences of Ml29. The relationship between mutation site and drug resistance phenotype was analyzed. Results A total of 50 Mp were isolated from 400 throat swab specimens. Of the 50 isolates, 32 isolates were susceptible to macrolide, and 18 isolates were resistant to macrolide. The 18 clinical isolates appeared mutation A2063G, A2064G and A2067G, separately. A2063G showed 14 ring macrolide resistance. A2064G showed 14 and 16 ring macrolide resistances. A2067G showed josamycin resistance. Conclusions Mp to macrolide resistance is serious, and the mutation of 23SrRNA gene is a predominant mechanism that contributes to the macrolide resistance. Through the analysis of 23SrRNA gene mutation site and drug resistance phenotype, the clinical Mp drug resistance situation is obtained. The theoretical guidance for reasonable selection and application of antibiotics is provided.%目的 了解本地区社区呼吸道感染肺炎支原体(Mycoplasma pneumoniae,Mp)感染状况,探讨肺炎支原体对大环内酯类抗菌药物的耐药分子机制,并分析肺炎支原体耐药菌株23SrRNA基因突变位点与耐药表型之间的关系.方法 对400例社区获得性呼吸道感染患儿咽拭

  4. Risk factors of complications analysis for infants with mycoplasma pneumonia pulmonary%婴幼儿肺炎支原体肺炎肺外并发症的危险因素分析

    Institute of Scientific and Technical Information of China (English)

    沈睿; 高琳; 彭苏妍; 叶志琼

    2016-01-01

    目的:探讨婴幼儿肺炎支原体肺炎(MPP)肺外并发症的相关因素。方法将105例MPP 患儿根据是否合并肺外并发症分为肺外并发症组(72例)和无肺外并发症组(33例),对两组患儿一般情况相关因素以及疾病相关因素进行单因素分析,差异有统计学意义的因素进行 Logistic 回归分析。结果婴幼儿 MPP 肺外并发症发生率68.6%(72/105),患儿主要肺外受累系统是消化系统(54.2%)、心血管系统(44.4%)以及血液系统(33.3%)。与 MPP 肺外并发症相关的20个因素中,年龄、喂养方式(包括人工、混合、母乳)、发病季节、发热天数、肺炎支原体(MP)-IgM 滴度、C 反应蛋白、红细胞沉降率、开始使用大环内酯类药物时间两组比较差异有统计学意义(P<0.05)。多因素 Logistic回归分析显示,年龄≥2岁、发热天数≥7 d、MP-IgM 滴度≥1∶160、C 反应蛋白增高、红细胞沉降率加快是婴幼儿 MPP 发生肺外并发症的危险因素,母乳喂养和发病7 d 内使用大环内酯类药物是保护因素。结论婴幼儿 MPP 肺外并发症发生率较高,可累及多个系统,年龄较长、热程较长、MP-IgM滴度高、C 反应蛋白增高、红细胞沉降率加快以及既往病史较多的患儿肺外并发症发生率较高,临床要给予高度重视。%Objective To investigate the complications related factors in infants with mycoplasma pneumonia pulmonary(MPP). Methods According to the condition of pulmonary complications, 105 cases of infants MPP were divided into pulmonary complication group and no pulmonary complication group with 72 cases and 33 cases respectively,and the general related factors and disease related factors of two groups were analyzed. Results The incidence rate of pulmonary complication was 68.6% (72/105) in infants MPP, and the main involved extra-pulmonary systems were digestive system (54.2%), cardiovascular

  5. 肺炎支原体感染与高血压患者的血清流行病学关系研究%Investigation on the serum epidemiological relationship between Mycoplasma pneumoniae infection and hypertension

    Institute of Scientific and Technical Information of China (English)

    项燕凌; 兰青; 陈嘉利; 陈先华; 张振业

    2015-01-01

    OBJECTIVE To study the serum epidemiological relationship between Mycoplasma pneumoniae infection and high blood pressure ,so as to provide the basis for the prevention and treatment of hypertension .METHODS Totally 80 cases from Mar .2013 to Mar .2015 were studied .According to the presence of hypertension ,patients were divided into the hypertension group and the non‐hypertension group ,40 cases in each group .The Seroidia‐Mycoll method was used to detect MPIgG antibodies in sera and the SPSS18 .0 software was used for statistical a‐nalysis .RESULTS The hypertension group had 40 cases of M .pneumoniae (MP) infection ,the infection rate was as high as 100 .00% ,significantly higher than 50 .00% in the non‐hypertensive infection (P<0 .05) .Indexes such as the number of patients ≥50 years of age ,the number of male patients ,smoking ,high cholesterol rate ,body mass index (BMI) and waist‐hip ratio (WHR) in the hypertension group were all significantly higher than those in the non‐hypertensive group (P<0 .05) .M .pneumoniae infection and gender ,dyslipidemia ,overweight ,and high blood pressure had a positive additive model of interaction .Risk of high blood pressure was 1 .92 ,1 .7201 and 1 . 24 for the interaction of the above each two factors before adjustment of the above factors and 28% ,28% and 25%of the risk of suffering from high blood pressure were caused by the interaction at the co‐existence of two factors . After making adjustments of each factor separately ,the risk was 7 .006 ,4 .455 and 0 .477 respectively .At the co‐existence of two above factors ,50 .00% ,45 .00% and 15 .00% of the risk of suffering from high blood pressure were caused by the interaction .CONCLUSION M .pneumonia infection and hypertension is related ,and there is in‐teraction with other factors of hypertension .%目的:探讨肺炎支原体感染与高血压患者的血清流行病学关系,为临床提供预防和治疗依据。方法选择2013年3月-2015

  6. 小儿支原体肺炎病情轻重的相关因素研究%Factors Related to the Severity of Symptoms in Children Mycoplasma Pneumoniae Pneumonia

    Institute of Scientific and Technical Information of China (English)

    徐哲; 陈华英; 马兵; 石平; 聂永红; 冯德刚; 刘亚军; 文虹; 刘燕

    2012-01-01

    with MPP were grouped by age: ^ 2 years of age ( 28 patients ), 3 ~ 5 years of age ( 79 patients ), and ^ 6 years of age ( 79 patients ) . They were also grouped by pneumonia pattern: bronchopneumonia group ( 94 patients ) and segmental/lobar pneumonia group ( 92 patients ) . In addition, the children aged ^ 6 years (79 patients ) were classified into three groups based on the severity of pneumonia: bronchopneumonia group (25 patients), mild segmental/lobar group (31 patients), and severe segmental/lobar group ( 23 patients). Ages, clinical symptoms, pulmonary lesions, laboratory findings and seroconversion rate of the 186 children with MPP were retrospectively analyzed. Results The fever in the three age groups all lasted ^7 days. There were significant differences among the three different age groups in bronchopneumonia, segmental/lobar, white blood cell count, neutrophil, lymphocyte, platelet count and C - reactive protein values ( P <0. 05) . The age, duration of fever, hospitalization, WBC count, neutrophil, lymphocyte, absolute lymphocyte count, platelet count, CRP, ESR and seroconversion rate were significantly different between the bronchopneumonia group and the segmental/lobar group ( P < 0. 05 ) . Hospitalization, CRP and seroconversion rate were significantly different among the bronchopneumonia group, the mild segmental/lobar group and the severe segmental/lobar group ( P < 0. 05 ). Conclusion In MPP, older children had longer fever and more severe pulmonary lesions. The severity of pulmonary lesions was associated with the absence of diagnostic IgM antibodies and lymphocyte count. Rapid pairing IgM serologic test is of great importance for early diagnosis of MPP.

  7. 阿奇霉素间歇治疗42例婴幼儿支原体肺炎的疗效及安全性分析%Analysis on the Curative Effect and the Safety of Azithromycin Intermittent Treatment for 42 Cases of Infant and Young Children With Mycoplasma Pneumonia

    Institute of Scientific and Technical Information of China (English)

    李琴

    2016-01-01

    目的:研究阿奇霉素间歇用药和连续用药治疗婴幼儿支原体肺炎临床效果和安全性。方法严格按照纳入标准抽选2013年1月~2016年1月我院收治的儿科支原体肺炎患儿84例作为研究对象,在以治疗方式为前提,按照随机数字表法将患儿进行分组治疗,治疗组42例采用阿奇霉素间歇治疗,对照组42例采用阿奇霉素连续治疗。观察两组患儿在治疗前后的临床效果变化、不良反应情况与支原体的转阴情况,并进行对比分析。结果治疗组患儿的支原体转阴率(92.86%)较对照组(76.19%)高,治疗总有效率(97.62%)高于对照组(83.33%),治疗组不良反应发生率为4.76%,对照组为14.29%,治疗组比对照组低;两组的组间数据由统计学分析χ2检验,差异具有统计学意义(P<0.05)。结论阿奇霉素间歇疗法治疗婴幼儿支原体肺炎能够迅速见效,改善支原体感染引起的症状,优于连续治疗方式;加速支原体转阴,治愈率高,同时不良反应轻微,婴幼儿的治疗依从性良好,减轻患儿的痛苦,临床疗效和安全性显著,在治疗中起到重要作用。%Objective To investigate the clinical efficacy and safety of intermittent and continuous administration of azithromycin in the treatment of mycoplasma pneumonia in infants.MethodsA total number of 84 children with mycoplasma pneumonia hospitalized in Pediatrics of our hospital were selected from January 2013 to January 2016 and included in this study. Patients were randomly divided into the treatment group (42 cases) and the control group (42 cases),with intermittent and continuous administration of azithromycin,respectively. The clinical efficacy,adverse reactions,and negative mycoplasma of pre-treatment and post-treatment in both groups were observed and compared.Results The negative rate of mycoplasma of children in treatment gorup (92.86%) was significantly higher than

  8. Experimental studies on the pathogenicity of Mycoplasma ovipneumoniae and Mycoplasma arginini for the respiratory tract of goats.

    Science.gov (United States)

    Goltz, J P; Rosendal, S; McCraw, B M; Ruhnke, H L

    1986-01-01

    Mycoplasma ovipneumoniae and Mycoplasma arginini were the species of Mollicutes most commonly isolated from 175 goats with respiratory disease in Ontario. The pathogenicity of M. ovipneumoniae, strain B321B and M. arginini, strain D53e, was assessed in goats following endobronchial inoculation. One out of three two year old goats developed fever after inoculation with a pure culture of strain B321B, and it had extensive subacute fibrinous pleuritis when necropsied three weeks later. Neither of the remaining goats had lesions in the respiratory tract. Mycoplasma ovipneumoniae was recovered from one of the animals four days after inoculation, but not at necropsy from any of the goats, at which time a marked humoral immune response with growth inhibiting antibodies was detected. In a second experiment three four to five week old goats were inoculated with the same strain and three other goats were given placebo treatment. One experimental goat developed fever and coughing, and it had extensive subacute fibrinous pleuritis in the right side and pneumonia. Another goat had focal pneumonia in the left diaphragmatic lobe. Microscopically there was subacute hyperplastic suppurative bronchiolitis, atelectasis and nonsuppurative alveolitis. The infected animals did not clear the mycoplasma and not all of them produced antibodies. Mycoplasma arginini, strain D53e, did not induce lesions in any of four goat kids within 14 days after inoculation but did cause transient elevations in rectal temperature, circulating monocytes, circulating neutrophils and blood fibrinogen. Mycoplasma arginini was infective and immunogenic for all inoculated animals and showed a particular affinity for the tonsil. Thus, this study provides the first evidence that M. ovipneumoniae is pathogenic for goats causing pneumonia and pleuritis.(ABSTRACT TRUNCATED AT 250 WORDS)

  9. Mycoplasma alkalescens demonstrated in bronchoalveolar lavage of cattle in Denmark

    Directory of Open Access Journals (Sweden)

    Ahrens Peter

    2007-01-01

    Full Text Available Abstract Mycoplasma alkalescens is an arginine-metabolizing mycoplasma, which has been found in association with mastitis and arthritis in cattle. Routine bacteriological examination of 17 bronchoalveolar lavage samples from calves with pneumonia in a single herd in Denmark, identified M. alkalescens in eight samples. The organism was found as a sole bacterilogical findings in five of the samples as well as in combination with Mannheimia haemolytica, Haemophilus somni and Salmonella Dublin. This is the first report of isolation of M. alkalescens in Denmark.

  10. Clinical symptoms of children with Mycoplasma pneumoniae infection-induced asthma and treatment measures%肺炎支原体感染引发小儿哮喘临床症状及治疗分析

    Institute of Scientific and Technical Information of China (English)

    张敏; 景霞; 傅大林

    2014-01-01

    目的:分析肺炎支原体感染引发小儿哮喘临床资料,并探讨治疗方法,为提高疗效提供依据。方法以2012年3月-2013年3月确诊的小儿哮喘患儿80例作为研究对象,其中40例肺炎支原体感染引发的哮喘患儿作为观察组,40例非肺炎支原体感染诱发哮喘的患儿为对照组,分别对两组患儿临床症状进行观察记录,并进行分析。结果40例观察组患儿发热33例占82.5%,其中体温>37.3℃17例占42.5%,体温>38℃14例占35.0%,体温>39℃2例占5.0%;40例对照组患儿发热18例占45.0%,其中体温>37.3℃14例占35.0%,体温>38℃4例占10.0%;观察组与对照组患儿的喘息发生特点、发热、肺部感染等临床表现不同。结论观察组患儿比对照组患儿的症状更加严重,在对肺炎支原体感染引发哮喘的患儿进行治疗时应采用大环内酯类抗菌药物、支气管扩张剂和糖质激素吸入治疗相结合的方式,并辅以日常护理治疗。%OBJECTIVE To analyze the clinical data of the children with asthma induced by Mycoplasma pneumoni‐ae infection and explore the treatment measures so as to provide guidance for the improvement of efficacy . METHODS From Mar 2012 to Mar 2013 ,a total of 80 children with conformed asthma were recruited as the study objects and were divided into the observation group with 40 cases (the children with M .pneumoniae infection‐in‐duced asthma) and the control group with 40 cases (the children with non‐M .pneumoniae infection‐induced asth‐ma);the clinical symptoms of the two groups of children were observed and recorded .RESULTS Of the 40 children in the observation group ,33 (82 .5% ) cases were with fever ,among whom the children with the body temperature more than 37 .3 ℃ accounted for 42 .5% (17 cases) ,the children with the body temperature more than 38 ℃35 .0% (14 cases) ,the children with the body temperature

  11. 肺炎支原体感染后小儿血常规的变化及临床意义%Mycoplasma Pneumonia Infection in Children after Blood and Its Clinical Significance

    Institute of Scientific and Technical Information of China (English)

    陈飞

    2014-01-01

    目的:观察小儿发生肺炎支原体(MP)感染后血常规的变化情况,探讨血常规检查的临床意义。方法以2012年1月至2012年12月我院收治的90例 MP 感染患儿为观察组研究对象,再选同期在我院作健康体检的90名健康幼儿为对照组对象。两组均给予血常规检查。结果观察组的红细胞计数、血小板计数血红蛋白含量与对照组相比差异不显著(P>0.05),但是白细胞计数、中性粒细胞比例与绝对值、核细胞比例与绝对值均比对照组有显著升高,比较差异具有统计学意义(P <0.05)。淋巴细胞比例及绝对值显著低于对照组(P <0.05)。结论通过实施血常规检测与比较,可以为小儿 MP 感染诊断提供重要依据,临床意义重大。%Objective To investigate the occurrence of mycoplasma pneumonia in children (MP) changes in the blood after infection, and to explore the clinical significance of blood tests. Methods From January 2012 to December 2012 in our hospital 90 cases of MP infection in children in the observation group study, then select the corresponding period in the hospital for physical examination of 90 healthy children as a control group of objects. Both groups were given blood tests. Results The observation group red blood cell count, platelet count and hemoglobin content compared with the control group had no significant difference (P>0.05), but the white blood cell count, europhile percentage and absolute value, the absolute value of the ratio of nucleated cells than the control group had significantly increased, and the difference was statistically significant (P<0.05). And the absolute lymphocyte ratio was significantly lower than the control group (P<0.05). Conclusion The implementation of blood testing and comparison can provide an important basis for the diagnosis of MP infection in children, the clinical significance.

  12. Related risk factors of cardiovascular system damage in children with mycoplasma pneumonia%儿童支原体肺炎心血管系统损害相关因素分析

    Institute of Scientific and Technical Information of China (English)

    李碧莹

    2015-01-01

    目的:探讨支原体肺炎患儿心血管系统损害的临床特点及相关危险因素。方法回顾性分析自2013年1月至2014年6月期间宁波市鄞州区茅山社区卫生服务中心收治的189例支原体肺炎患儿的临床资料,采集患血管系统损害的临床表现,并对发生心血管系统损害的相关危险因素进行单因素以及多因素Logistic回归分析。结果在189例肺炎支原体感染患儿中,共有42例并发肺外心血管系统损害,发病率为22.2%;多因素Logistic回归分析提示:年龄(OR=2.452)、热程(OR=1.337)、C-反应蛋白(OR=1.563)、发热程度(OR=1.310)、大环内酯类抗生素应用起始时间(OR=2.452)与支原体肺炎患儿肺外心血管系统损害均呈正相关(均P<0.05),而CD4+/CD8+比值升高(OR=0.679)则为保护性因素(P<0.05)。结论年龄、热程、高C-反应蛋白、发热程度、大环内酯类抗生素应用起始时间及低血清CD4+/CD8+比值是肺炎支原体感染患儿肺外心血管系统损害的高危因素,临床应根据这些高危因素制定防止措施以改善患儿的预后。%Objective To investigate the clinical characteristics of cardiovascular system damage in children with mycoplasma pneumoniae ( MP) and related risk factors. Methods A retrospective analysis was conducted on the clinical data of 189 children with MP treated during the period of January 2013 to June 2014 in Ningbo Yinzhou District Maoshan Community Health Service Center, and clinical manifestations of cardiovascular system damage were collected. Related risk factors of cardiovascular system damage were analyzed by univariate and multivariate Logistic regression analysis. Results Of 189 cases with MP, 42 children were complicated with cardiovascular system damage, and the incidence was 22. 2%. Multivariate Logistic regression analysis showed that age (OR=2. 452), course of fever (OR=1. 337), CRP (OR=1. 563), degree of fever (OR=1. 310) and starting time of

  13. Real-time PCR检测肺炎支原体对大环内酯类抗生素耐药基因%Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR

    Institute of Scientific and Technical Information of China (English)

    沈俊娅; 卢忠; 盛文彬; 陈岳明

    2009-01-01

    目的 探讨双重荧光实时聚合酶链反应(real-time PCR)在肺炎支原体(Mycoplasma pneumoniae,MP)对大环内酯类抗生素耐药基因检测中的应用.方法 19例自临床咽拭子样品分离培养的肺炎支原体及标准菌株,FH,进行体外药物敏感试验,并用自行设计的双重荧光Real-time PCR检测大环内酯类抗生素耐药基因型,同时对相应序列测序分析.抽提127份经PCR测定MP阳性的临床样品基因组DNA,用MP阴性的基因组DNA抽提物5份做阴性对照,用Real-time PCR检测大环内酯类抗生素耐药基因型.结果 19例分离培养的MP,体外药物敏感试验结果显示15株有耐药,并且都有23S核糖体RNA(rRNA)V区基因A2063G位点突变;而4株对大环内酯类抗生素敏感的MP基因型为23S rRNA V区基因2063A;测序结果证实Real-time PCR结果.127份MP阳性的基因组DNA抽提物,5份用MP阴性的基因组DNA抽提物,经Real-time PCR检测,其中122份样品为MP 23S rRNA V区基因A2063G位点突变,2份样品的2063位点碱基为A,还有3份样品的2063位点碱基既有A又有G.127份MP阳性的样品在Real-time PCR检测中未见到2条荧光曲线都不生长的情况.5份阴性对照2条荧光曲线都不生长.结论 双重荧光 Real-time PCR能为临床快速、准确检测MP对大环内酯类抗生素耐药的基因型.

  14. Application value of PCR spot hybridization technique in detection of mycoplasma pneumoniae infection%PCR 斑点杂交技术在肺炎支原体感染检测中的应用价值

    Institute of Scientific and Technical Information of China (English)

    张秀娟

    2016-01-01

    Objective To investigate the application value of PCR spot hybridization technique in the detection of mycoplasma pneumoniae(MP) infection .Methods The DNA of 70 respiratory tract specimens from the children with MP infection was extracted for MP specific DNA amplification by PCR .The detection efficiency of MP infection was compared between traditional PCR method and PCR spot hybridization technique .Results Of 70 specimens ,32 specimens were MP positive and 28 specimens were MP negative detected by traditional PCR method and PCR spot hybridization technique .The MP positive was detected by PCR spot hybridization technique in 10 specimens ,which were MP negative by traditional PCR method (P<0 .05) .The sensitivity ,specificity and diagnostic coincidence rate of MP detection by PCR spot hybridization technique were 82.5% ,95.6% and 81.4% ,respectively ,which were higher than 69.6% ,80.6% and 50.2% by traditional PCR method . Conclusion Compared to traditional PCR method ,PCR spot hybridization technique has higher sensitivity and specificity in the detection of M P infection .%目的:探讨 PCR斑点杂交技术在检测肺炎支原体(M P )感染中的临床应用价值。方法收集疑似MP感染患儿的呼吸道标本70份,提取DNA后采用PCR技术将MP特异性DNA片段进行扩增;比较传统PCR法和PCR斑点杂交技术检测M P感染的效能。结果32份临床标本经传统PCR法和PCR斑点杂交技术检测M P均为阳性,28份临床标本检测均为阴性;10份临床标本PCR斑点杂交技术检测为阳性,但传统 PCR法检测为阴性,配对样本χ2检验显示两法检测M P的差异有统计学意义(P<0.05)。 PCR 斑点杂交技术检测 MP 的灵敏度为82.5%,特异度为95.6%,诊断符合率为81.4%;均高于传统PCR法的69.6%、80.6%和50.2%。结论与传统PCR法比较,PCR斑点杂交技术检测M P的灵敏度和特异度高。

  15. Epidemiological and clinical analysis of Mycoplasma pneumoniae infection in children with acute respiratory tract infection%呼吸道感染患儿肺炎支原体感染流行特点和临床分析

    Institute of Scientific and Technical Information of China (English)

    许蔓春; 马恒颢; 欧巧群; 罗爱武; 任广立; 王鲜艳; 荆丽娟

    2009-01-01

    Objective To summarize the epidemiology and clinical characteristics of Mycoplasma pneumoniae (MP) infection in children with acute respiratory tract infection (ARI) in Guangzhou. Methods MP was detected using an indirect immunofluorescent method in 2084 children with ARI. The relations between MP infection rate and the gender, age, season, site of infection and wheezing diseases were analyzed. Results A total of 433 children (20.8%) were positive for MP, including 222 boys (19.8%) and 211 girls (21.9%) without significant difference in the infection rate between the genders (P>0.05). In 0-to 3-year-old group, 106 children were positive for MP (15.0%), while in 3-to 5-year-old group and 5-to 14-year-old group, 163 (25.2%) and 164 (22.5%) were positive, respectively, showing a significant difference in the infection rate between the 3 groups (P0.05). Among the children with LRI, those having wheezing disease had significantly higher MP positivity rate than those without wheezing. Conclusion MP is a common causative agent for ARI in children. MP infection is not related to gender and infection site, but to age and season. Children over 3 years old are vulnerable to MP infection. MP infection can be associated with wheezing in LRI.%目的 探讨小儿呼吸道感染者肺炎支原体(MP)感染的流行特点和临床情况.方法 回顾性分析我院2004~2008年呼吸道感染住院患儿2084例,采用间接免疫荧光法检测MP,分析MP感染率与性别、年龄、季节、部位及与喘息性疾病的关系.结果 2084例呼吸道感染患儿中MP阳性患儿433例(20.8%),其中男性222例(19.8%),女性211例(21.9%),男性与女性MP发病率无统计学差异(P>0.05).不同年龄组MP发病率分别为:小于3岁组106例(15.0%),3~5岁组163例(25.2%),5~14岁组164例(22.5%),三组之间MP感染率差异有统计学意义(P<0.05).不同季节MP感染率分别为:1~3月:18.0%,4-6月:25.1%,7~9月:17.7%,10~12月:20.5%,不同季节之间MP感染率

  16. Características clínicas y epidemiológicas de pacientes pediátricos con serología positiva para Mycoplasma pneumoniae, atendidos en un hospital de tercer nivel durante el período 2006-2008

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    Margarita María Suárez

    2011-06-01

    Full Text Available Introducción: La infección por Mycoplasma pneumoniae es cada vez más frecuente a edades tempranas. Se presenta como un cuadro clínico de síntomas respiratorios generalmente benigno e inespecífico y en ocasiones es reponsable de un amplio espectro de manifestaciones extrapulmonares. Objetivo: Describir las características demográficas, clínicas, de laboratorio y el tratamiento de pacientes en edad pediátrica con serología positiva para M. pneumoniae diagnosticados en el Hospital Pablo Tobón Uribe (HPTU durante el período 2006-2008. Materiales y métodos: Estudio observacional, retrospectivo, que describe las características demográficas, clínicas, de laboratorio y tratamiento de pacientes de 1 mes a 14 años de edad con IgM positiva para M. pneumonie atendidos en el HPTU entre septiembre de 2006 y noviembre de 2008. Resultados: Se revisaron 305 historias clínicas de pacientes con IgM positiva para M. pneumoniae. La edad promedio fue 5.15 años ± 3.62 DE. La tos (87.5%, fiebre (65.9%, rinorrea (39.3% y dificultad respiratoria (38% fueron los hallazgos clínicos más frecuentes. El compromiso extrapulmonar se manifestó sobre todo en piel (12.8%, sistema nervioso (3.6% y sistema osteomuscular (3.9%. La auscultación y la radiografía de tórax fueron normales en 33.1% y 30.8% respectivamente. Los episodios de exacerbación del asma se relacionaron con infección por Mycoplasma en 35% de los casos. Del total de niños, 51% necesitó hospitalización con un promedio de estancia de 7 días. Todos los pacientes recibieron tratamiento antibiótico, siendo el de elección claritromicina (75.8%. Conclusión: La infección por M. pneumoniae posee una amplia gama de manifestaciones clínicas que requiere un alto índice de sospecha clínica y una búsqueda activa de compromiso extrapulmonar porque en Colombia no se cuenta con un método diagnóstico específico para la infección aguda.

  17. Characterization of Mycoplasma penetrans and Mycoplasma fermentans immunodominant proteins Caracterização de proteinas imunodominantes de Mycoplasma penetrans e Mycoplasma fermentans

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    Juliana Bruder

    2005-06-01

    Full Text Available Mycoplasmas are a heterogeneous group of the smallest organisms capable of self replication and are known to cause many detrimental diseases in both animals and humans. These wall-less prokaryotes are enveloped by a lipoprotein membrane and their small genomes are sufficient to synthesize molecules required for growth and self-replication. Among sixteen species isolated from humans, Mycoplasma pneumoniae, an agent of primary atypical pneumonia, and the urogenital tract species Mycoplasma hominis,Ureaplasma urealyticum and Ureaplasma parvum have been confirmed to be pathogenic. Mycoplasma penetrans and Mycoplasma fermentans, which are species associated with HIV, have been investigated mainly in research laboratories. In this study we have characterized lipid-associated membrane proteins (LAMP of Mycoplasma penetrans and Mycoplasma fermentans, in view of the importance of mycoplasmas in human diseases and the peculiar antigenic variation observed in these species. To characterize proteins with possible diagnostic value, we used ELISA and Western blot in sera of pregnant women whose cervical samples were positive for these species of mycoplasmas when tested by PCR. ELISA showed IgG anti-LAMP-M. fermentans antibodies to be present in 57.5% of cases and IgM antibodies to be present in 74.5% of cases. The three samples that were PCR positive for M. penetrans showed IgG anti-LAMP-M. penetrans antibodies, and one sample was positive for IgM. No IgA antibodies against either species were detected in any of the samples. LAMP analysis by Western blot revealed the 35, 38, 42, 61 and 103 kDa proteins of M. penetrans and the 29, 38, 41, 61, 78 and 95 kDa proteins of M. fermentans. Among these, will be considered p35 to M. penetrans and 29 kDa protein to M. fermentans, the main immunoreactive proteins and therefore useful markers for further laboratory diagnosis.Micoplasmas são procariotos diminutos, desprovidos de parede celular e envoltos por uma membrana

  18. Vaccines for Mycoplasma diseases in animals and man.

    Science.gov (United States)

    Nicholas, R A J; Ayling, R D; McAuliffe, L

    2009-01-01

    Vaccines for important mycoplasma diseases, including contagious bovine and caprine pleuropneumonia, have been used for centuries, consisting mainly of infected tissue or fluids which are inoculated into sites at which the risk of severe infection is slight, such as the tail and bridge of the nose. Surprisingly, little progress has been made in developing safe, defined and protective alternatives, the vaccines today still consisting of mildly attenuated strains serially passaged in eggs or in culture. Ill-defined temperature-sensitive mutants are widely used for mycoplasmoses in poultry despite uncertainty about their mode of protection. Inactivated vaccines for enzootic pneumonia appear to have improved pig health worldwide, but disease reduction has been generally modest. Ironically, attempts to develop subunit preparations have often led to exacerbation of disease, particularly in human atypical pneumonia. Promising results have been seen in DNA vaccine technology, which has been applied to the development of mycoplasma vaccines for porcine enzootic pneumonia, but field trials still seem a long way off. No commercial vaccines exist for Mycoplasma bovis, despite evidence that this is a major cause of calf pneumonia, mastitis and arthritis.

  19. Chlamydophila abortus em animais de produção Chlamydophila abortus in production animals

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    Francielle Gibson da Silva

    2006-02-01

    Full Text Available A Chlamydophila abortus (anteriormente classificada como Chlamydia psittaci sorotipo 1 tem sido descrita em muitos países, associada principalmente com distúrbios reprodutivos em ovinos, bovinos e caprinos. O aborto enzoótico dos ovinos e caprinos e o aborto epizoótico dos bovinos são as doenças mais importantes causadas por esta bactéria. No Brasil, as pesquisas com C. abortus são praticamente inexistentes. O objetivo desta revisão é apresentar informações sobre modificações taxonômicas, ciclo de vida, epidemiologia, patogenia, sinais clínicos e diagnóstico da infecção por C. abortus principalmente em ovinos, bovinos e caprinos.Chlamydophila abortus (previously known as Chlamydia psittaci serovar 1 has been reported in many countries, associated with reproductive disorders in sheep, cattle, and goats. The enzootic abortion of sheep and goats and the epizootic bovine abortion are the most important diseases produced by this bacterium. In Brazil, there is scarce information about C. abortus. The objective of this review is to show information about taxonomic changes, life cycle, epidemiology, pathogenesis, clinical signs and diagnosis of C. abortus in sheep, cattle and goats.

  20. 2 cases report and review of adults severe mycoplasma pneumonia with anemia causing by cold agglutinin%冷凝集素介导贫血的成人重症支原体肺炎2例并文献复习

    Institute of Scientific and Technical Information of China (English)

    谭星宇; 公丕花; 董霄松; 韩芳; 高占成

    2013-01-01

    Objective To investigate the characteristic of adults severe mycoplasma pneumonia with anemia causing by cold agglutinin.Method Adults severe mycoplasma pneumonia with anemia causing by cold agglutinin.Result Case 1,male,28 y,cough phlegm 16 days,fever 14 days,anemia,icteric sclera,little moist rales.The chest radiograph shows multiple small nodules under double lung,patchy shadows in the right lower lung and right middle,and tree-in-bud sign.Red blood cell of venous blood coagulation after a few seconds and solution after 50℃ warm bath.Mycoplasma pneumonia antibody 1:20480.Coomb' s test was positive.Moxifloxacin,azithromycin and prednisone 30mg were treated.Case 2,female,18 y,with anemia,fever and cough 2 weeks,right-sided chest pain,1 weeks occasionally diarrhea.The chest radiograph showed patchy shadows in right lower lobe and right pleural effusion.The breath sounds of right lower lung decreased,without rale.Venous blood were found to have self coagulation phenomenon of red blood cells.Mycoplasma pneumoniae antibody was 1:1280.Loop mediated isothermal amplification detection mycoplasma.Coomb' s test was positive.Pleural effusion were exudative with ADA 44.1U/L.Azithromycin,methylprednisolone 40mg and low molecular weight heparin were treated.Conclusion Two cases were young people with fever more than 2 weeks,venous blood red blood cells condensatiog,anemia,Coombs' test positive,high mycoplasma pneumoniae antibody,nonspecific imaging findings.Only macrolide antibiotics and fluoroquinolone drugs had ineffective.It need treatment with glucocorticoid and anticoagulant when necessary.%目的 总结成人冷凝集素介导溶血性贫血的重症支原体肺炎病例的特点.方法 报道2例成人冷凝集素介导溶血性贫血的重症支原体肺炎病例.结果 病例1男,28岁,咳嗽、咳痰16天,发热14天,贫血,巩膜黄染,双肺可闻及细小湿哕音,胸片示双下肺多发小结节,右下肺和右中叶片状阴影,伴树芽征,入院时静脉

  1. 肺炎支原体快速培养检测法的临床应用价值评估%Evaluation of clinical application value of rapid culture and detection method of Mycoplasma pneumoniae

    Institute of Scientific and Technical Information of China (English)

    张广清; 戴洪法; 葛晶晶; 黄美琼; 刘玉线

    2016-01-01

    Objective To explore the value of Mycoplasma pneumoniae(MP) 6 h rapid detection method ,PCR detection method and serum antibody detection method in diagnosis of M P .Methods A total of 140 patients admitted in the respiration department of our hospital from August 2012 to August 2014 were selected as the research subjects .The throat swab on the next d of admission was collected for conducting the rapid identification medium detection ;2mL of vein blood was extracted from the patients for detec‐ting MP specific antibody by the ELISA method ;the MP fast medium was taken for conducting the amplification of MP gene frag‐ment by PCR .Results Among 140 cases ,43 cases of throat swab cultures were positive ,included 25 male cases and18 female ca‐ses ;the age ranged 2?14 years old ,in which 24 cases were pneumonia ,9 cases were acute bronchitis and 10 cases were chronic lung diseases .The positive rate in the MP rapid culture method was 30 .7% (43/140) ,which in the PCR detection method was 45%(63/140) ,the PCR detection method was significantly higher than the MP rapid culture method (χ2 =4 .347 ,P=0 .037);in the consistency test of the two methods ,kappa=0 .554 (t=6 .868 ,P=0 .000);the positive detection rate of MP specific antibody was 37 .9% (53/140) ,which had no statistical difference between the MP rapid culture method and the specific antibody detection (χ2 =1 .150 ,P=0 .284);in the consistency test of two methods ,kappa=0 .338(t=4 .050 ,P=0 .000) .Conclusion The MP 6 h rapid detection method has the advantages of short time and simple operation ,but there is still the possibility of false positive ,so certain errors may exist with which as the MP diagnostic index .%目的:探讨6 h快速检测法、PCR检测法、血清抗体检测法在肺炎支原体(M P)诊断中的价值。方法选择2012年8月至2014年8月呼吸科收治的140例患者作为研究对象,收集患者入院次日咽拭子进行快速鉴定培养基

  2. The Chlamydophila felis plasmid is highly conserved.

    Science.gov (United States)

    Harley, Ross; Day, Sarinder; Di Rocco, Camillo; Helps, Chris

    2010-11-20

    The presence of a plasmid in the Chlamydiaceae is both species and strain specific. Knowledge of the prevalence of the plasmid in different Chlamydia species is important for future studies aiming to investigate the role of the plasmid in chlamydial biology and disease. Although strains of Chlamydophila felis with or without the plasmid have been identified, only a small number of laboratory-adapted strains have been analysed and the prevalence of the plasmid in field isolates has not been determined. This study aimed to determine the prevalence of the plasmid in C. felis-positive conjunctival and oropharyngeal clinical samples submitted for routine diagnosis of C. felis by real-time (Q)PCR. DNA extracts from four laboratory-adapted strains were also analysed. QPCR assays targeting regions of C. felis plasmid genes pCF01, pCF02 and pCF03 were developed for the detection of plasmid DNA. QPCR analysis of DNA extracts from C. felis-positive clinical samples found evidence of plasmid DNA in 591 of 595 samples representing 561 of 564 (99.5%) clinical cases. Plasmid DNA was also detected by QPCR in laboratory-adapted strains 1497V, K2487 and K2490, but not strain 905. We conclude that the plasmid is highly conserved in C. felis, and plasmid-deficient strains represent a rare but important population for future studies of chlamydial plasmid function.

  3. Detection, Characterization, and Molecular Typing of Human Mycoplasma spp. from Major Hospitals in Cairo, Egypt

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    Mirihan A. Metwally

    2014-01-01

    Full Text Available Mycoplasmas are fastidious slow growing organisms lacking a cell wall and mostly isolated from the mucosal surfaces of the respiratory and genitourinary tracts. There is a dearth of information regarding clinical Mycoplasma spp. isolates among Egyptian patients. A total of 170 samples were collected from patients and apparently healthy personnel in local public hospitals in Cairo, Egypt. Isolation of Mycoplasma spp. was carried out using appropriate culture media and further identification was carried out by biochemical tests followed by serotyping using specific antisera. Confirmation was done by PCR for detection of different Mycoplasma spp. using genus-specific primers targeting 16S ribosomal RNA gene. Characterization of the antibiotic resistance and sensitivity pattern against different antimicrobials was carried out using disc diffusion test. The results indicated the presence of six Mycoplasma spp. in 22.94% of the samples. Mycoplasmas were detected more frequently in throat swabs than sputum. Mycoplasma pneumoniae was highly sensitive to macrolides and quinolones but less sensitive to aminoglycosides and tetracyclines. Molecular techniques were found to be of more rapid, highly sensitive, able to detect nonviable organisms, and cost effective. These results shed light on difficulties of Mycoplasma detection and the superiority of molecular techniques over culture.

  4. Genome sequence of the Chlamydophila abortus variant strain LLG.

    Science.gov (United States)

    Sait, Michelle; Clark, Ewan M; Wheelhouse, Nick; Livingstone, Morag; Spalding, Lucy; Siarkou, Victoria I; Vretou, Evangelia; Smith, David G E; Lainson, F Alex; Longbottom, David

    2011-08-01

    Chlamydophila abortus is a common cause of ruminant abortion. Here we report the genome sequence of strain LLG, which differs genotypically and phenotypically from the wild-type strain S26/3. Genome sequencing revealed differences between LLG and S26/3 to occur in pseudogene content, in transmembrane head/inc family proteins, and in biotin biosynthesis genes.

  5. 儿童急性呼吸道感染肺炎支原体抗体检测结果分析%Detection of Mycoplasma pneumoniae antibody in children with acute respiratory tract infections

    Institute of Scientific and Technical Information of China (English)

    郑曦

    2013-01-01

    目的 研究肺炎支原体(MP)感染发病率与季节、患儿年龄和性别的关系,了解MP流行趋势和致病特点,为临床诊断MP感染提供依据.方法 用被动凝集法检测呼吸道感染患儿血清中MP抗体(MP-Ab),并对2011年急性呼吸道感染患儿MP-Ab检测结果进行分析.结果 送检的5271份标本中有1603份检出阳性,阳性率为30.41%,其中MP-Ab检测滴度1:80的患儿所占比例最高为27.63%,滴度≥1:1280较少,占6.68%;MP感染的季节性明显,春季和冬季的检出率最高;在不同年龄组中,1~3岁组阳性率最高,为34.20%,其次是3~6岁组为31.25%,≥6岁组的阳性率最低为24.13%,各年龄组差异有统计学意义(P<0.05);男、女性患儿阳性率分别为30.96%、29.42%,差异无统计学意义.结论 MP感染症状较轻,大多患儿预后良好;全年均可发病,其中以冬春季高发;1~6岁儿童为高危人群;男、女患儿感染机会相当.%OBJECTIVE To study the relationship between the incidence of Mycoplasma pneumoniae (MP) infection and the season,age and gender of patients in Xianning and understand the prevalence of MP and the pathogenic characteristics so as to provide basis for the clinical diagnosis of MP infection.METHODS The serum MP-Ab were detected in the patients with respiratory tract infections by passive agglutination,and the MP-Ab detection results were analyzed in 2011.RESULTS Of 5271 cases of specimens,1603 cases were detected positive with the positive rate of 30.41 %,the heist proportion was in the children with the titer of 1:80 (27.63%),the children with titer more than 1:1280 were less,accounting for 6.68%;the seasonal nature of MP infection was obvious,and the highest detection rate was in spring and winter;In different age groups,the highest positive rate was in the 1 to 3-year-old group (34.20%),followed by 3 to 6-year-old group (31.25%),and the more than 6-year-old group (24.13 %),the difference

  6. The efficacy of glucocorticosteroid on the treatment of severe or refractory mycoplasma pneumoniae ;pneumonia%糖皮质激素在儿童重症或难治性肺炎支原体肺炎治疗中的价值

    Institute of Scientific and Technical Information of China (English)

    吴海飞; 檀卫平; 黄花荣; 吴葆菁; 韩明珊; 欧榕琼

    2015-01-01

    Objective To investigate the effect of glucocorticoid on the treatment of severe and re-fractory mycoplasma pneumoniae (MP)pneumonia in children.Methods Thirty seven patients (aged 2 months ~1 4 years,median 36 months)with severe or refractory MP pneumonia were divided into the two groups:treatment group (n =22)and control group (n =1 5).The patients in the two groups were treated with routine oral or intravenous azithromycin,and received the combined therapy including oxygen inhalation,atom-ization inhalation,and postural drainage.The patients in the treatment group were additionally treated with in-travenous methylprednisolone (daily,2 ~3 mg/kg)for 3 ~5 days,and then with prednisone (daily,1 ~2 mg/kg)orally and gradually reduced,maintained 7 ~1 0 days.Differences of clinical symptoms,and hospitali-zation time were compared between the two groups.Results The symptom of all the children were significantly improved,the patients’condition was significantly improved and discharged.The hospitalization time of the patients in the treatment group was decreased compared with that of the patients in the control group (8.45 ± 3.1 4 vs.1 1 .07 ±2.74,P <0.05).The total duration of fever of the patients in the treatment group was shor-ter compared with that of the patients in the control group (4.52 ±2.77 vs.6.80 ±3.76,P <0.05).The cough improve time of the patients in the treatment group was decreased compared with that of the patients in the control group (3.48 ±1 .37 vs.4.53 ±1 .49,P <0.05).Conclusions The use of suitable dosage and short treatment period of corticosteroids contributes to reduce the therapy time of severe or refractory MP pneu-monia in children.%目的:探讨糖皮质激素在儿童重症和难治性肺炎支原体(MP)肺炎治疗中的作用。方法将37例2个月~14岁(中位数36个月)的重症或难治性 MP 肺炎患儿按照是否使用糖皮质激素分为治疗组22例、对照组15例。2组患儿入院后均常规予以口

  7. Molecular epidemiology of C. pneumoniae infections

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    Alisa Shurdhi

    2010-06-01

    Full Text Available Introduction. Chlamydophila pneumoniae (C. pneumoniae is one of the most common respiratory pathogen, with an incidence of infection varying from 6% to 20%. The present study aimed to assess the incidence of C. pneumoniae infections in patients with acute respiratory diseases using a RealTime PCR (RT-PCR method. Methods. In the period January 2007-December 2008 279 biological samples coming from patients (190 males and 89 females with acute respiratory infections was collected and tested. Samples have been extracted using NucliSens easyMag Biomerieu according to manufacturer’s instructions and amplified by LightCycler Real-Time PCR Roche for the detection of C. pneumoniae DNA. Results. Data analysis revealed a higher prevalence of C. pneumoniae infections in male patients (7.9% than in females (5.6%. In addition, it is interesting to note that the incidence of C. pneumoniae infection was higher 28.6% in the period February - April. Conclusions. The results obtained in this study confirm the utility of molecular techniques in laboratory diagnosis and epidemiological investigations of respiratory infection caused by C. pneumoniae. RT-PCR have proved to be a rapid and a reliable technique to monitor and treat opportunely C. pneumoniae infections to avoid short and medium/long term complications.

  8. Analysis of Specific Allergens IgE and the Relationship with Mycoplasma Pneumoniae Infection in Allergic Disease Children%儿童过敏性疾病变应原特异性IgE及其与肺炎支原体感染相关性分析

    Institute of Scientific and Technical Information of China (English)

    刘玭; 吴星; 梅娟; 徐之良

    2011-01-01

    目的 探究儿童过敏性疾病中特异性变应原分布及规律,与肺炎支原体感染的相关性,指导临床防治.方法 采用酶联免疫法对199例武汉地区过敏性疾病儿童进行吸人性和食人性变应原的血清学检测.间接免疫荧光法对肺炎支原体(MP)感染进行检测.结果 199例患儿中吸人性变应原阳性97例(48.7%).年龄≥3岁组高于年龄<3岁组,差异有统计学意义(P<0.05).其中尘、粉螨83例(41.7%)最高;食人性变应原阳性70例(35.2%),按年龄分组差异无统计学意义,以鱼、虾、蟹27例(13.6%)最多.秋季人院率(69例,34.7%)最高,夏季入院率相对最低.吸人性变应原阳性与肺炎支原体感染有统计学相关性(P<0.05).结论 过敏性疾病中吸人性变应原为主且阳性率随年龄增长而增加.尘、粉螨及鱼、虾、蟹为主要变应原,秋冬春季为过敏性疾病好发季节,肺炎支原体感染与过敏性疾病有关.%Objective To explore the distribution of main inhalational and alimentary allergens which trigger children allergic disease, and the relationship between mycoplasma pneumoniae infection and allergy. Methods Serum specific allergens IgE in 199 children in Wuhan with allergic disease children were detected with ELISA and indirect immunofluorescence including inhalational alimentary allergens and mycoplasma pneumoniae infection. Results The total positive rate of inhalational allergens of patients was 48.7% (97/199). The positive rate of aspiration allergens increased with the patient's age and showed a positive correlation ( P <0. 05). The highest rate was dust mite 41.7% (83 cases). The positive rate of food allergens was 35.2% (70/199). Among them, the highest rate were fish, shrimp and crab 13. 6% (27 cases) . The positive rate of alimentary allergens had no significant difference in the two age different group. Autumn was the highest occurrence seasons while summer was the lowest. The positive rate of

  9. The clinical observation of treating mycoplasma pneumoniae with budesonide and ambroxol atomization%布地奈德氨溴索雾化吸入治疗小儿肺炎支原体感染临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    鲁奇志; 张朝勇

    2013-01-01

    Objective: To study the clinical effect of treating mycoplasma pneumoniae with budesonide and ambroxolatomization. Methods: Choosed 200 cases of mycoplasma pneumoniae to divide into study group and control ed group with 100 cases in each other. The control ed group were treated with ambroxol atomization,the study group were treated with budesonide atomization added. Compared the clinical effect of these two groups. Results: The response duration of study group was shorter than control ed group (P<0.05). The effective rate of study group was 92.0%, higher than 75.0% in control ed group. Conclusion: Treating mycoplasma pneumoniae with budesonide and ambroxol atomization can receive good effects with high safety to extend in clinic.%目的:研究分析对小儿肺炎支原体感染患儿行盐酸氨溴索联合布地奈德雾化吸入治疗的临床疗效。方法:回顾性分析2010年2月-2012年10月期间,我院收治的200例小儿肺炎支原体感染患儿的临床资料,按照患儿入院治疗的先后顺序及采用的治疗方案不同,将200例患儿分为两组,对照组100例,采用单纯用药方式治疗(盐酸氨溴索雾化吸入),观察组患儿采用联合用药的方式治疗,在对照组患儿采用的治疗方式的基础上加用布地奈德雾化吸入治疗。比较两组患儿的临床症状改善情况和治疗效果。结果:观察组患儿治疗后的临床症状缓解时间明显短于对照组,两组比较差异明显,具有统计学意义,(P<0.05),观察组患儿的治疗有效率为92.0%,对照组患儿的治疗有效率为75.0%,两组比较差异显,具有统计学意义,(P<0.05)。结论:布地奈德氨溴索雾化吸入治疗小儿肺炎支原体感染的临床效果明显,且治疗后患者的症状缓解明显,安全性高,值得临床推广。

  10. Pulmicort Respules Combined with Azithromycin in the Treatment of Clinical Observation of 102 Children ;with Mycoplasma Pneumoniae Infection%普米克令舒联合阿奇霉素治疗102例儿童肺炎支原体感染的疗效观察

    Institute of Scientific and Technical Information of China (English)

    户波; 毋英超

    2015-01-01

    目的:探讨和分析普米克令舒联合阿奇霉素治疗儿童肺炎支原体感染的疗效观察。方法:从本院2012年12月-2013年12月收治的支原体肺炎患儿中随机抽取102例作为本次研究对象,按随机双盲法将102例患儿分为观察组与对照组,对照组患儿51例,采用阿奇霉素对其进行治疗,观察组患儿51例在对照组的治疗基础上加入普米克令舒,观察两组患儿的临床疗效、住院时间、病症消失时间、不良反应。结果:观察组患儿治愈率与总效率分别为52.94%(27例)、94.11%(48例),明显高于对照组的31.37%(16例)、80.39%(41例),比较差异有统计学意义(P<0.05)。观察组患儿住院时间、病症消失时间及不良反应明显低于对照组,比较差异有统计学意义(P<0.05)。结论:普米克令舒联合阿奇霉素治疗儿童支原体肺炎疗效显著,且住院时间及不良反应都较少,得到了患儿家属的一致好评,值得在临床上进行推广并应用。%Objective:To discuss and analyze Pulmicort Respules combined with azithromycin clinical observation on the treatment of Mycoplasma pneumoniae infection in children.Method:102 cases with mycoplasma pneumonia were randomly selected in our hospital from 2012 December to 2013 December period as the object of study, according to the random double blind method,102 patients were divided into observation group and control group,control group contained 51 cases,using azithromycin,the observation group contained 51 cases added Pulmicort treatment on the basis of the control group,the clinical efficacy after treatment of the two groups was observed,as well as hospitalization time,symptoms disappear time,adverse reaction.Result:The cure rate and total effective rate of the observation group was 52.94%(27 cases),94.11%(48 cases)respectively,which was significantly higher than 31.37%(16 cases), 80.39%(41 cases)of the control group,there were

  11. 脾氨肽口服冻干粉对小儿支原体感染肺炎伴哮喘临床效果及C反应蛋白的影响%The Clinical Effect and the Influence of CRP of Spleen Peptide Oral Ammonia Producing for Pediatric Pneumonia Mycoplasma Infection Associated with Asthma

    Institute of Scientific and Technical Information of China (English)

    陈克娅; 冯涛

    2016-01-01

    Objective:To observe the clinical effect and the influence of c-reactive protein of ( CRP ) spleen peptide oral ammonia producing for pediatric pneumonia mycoplasma infection associated with asthma. Methods:The data of 124 children with mycoplasma pneumonia with asthma admitted in our hospital from June 2015 to June 2016 were analyzed. According to the different treatment schemes, all the children were di-vided into controll group and observe group. The control group were treated with anti-inflammatory treatment with azithromycin, and the observation group were treated with spleen peptide oral ammonia producing. The clinical effects and c-reactive protein ( CRP ) expression of two groups were compared. Results: The impro-ving effects of IgA, IgG and T lymphocyte subgroup after cure in the observation group were better than that in the control group. The difference was statistically significant (P<0.05). The level of CRP, WBC and creati-nine in the observation group were lower than the control group ( P<0.05) . Conclusion:Mycoplasma pneumo-nia infection associated with asthma role of spleen peptide oral ammonia producing heal can optimize clinical effect, reduce the children c-reactive protein (CRP) levels, it is worth promoting.%目的:研究支原体感染肺炎伴哮喘患儿行脾氨肽口服冻干粉医治对临床效果和C反应蛋白的影响.方法:前瞻性取2015年6月至2016年6月本院收治支原体感染肺炎伴哮喘124例患儿资料进行分析,按不同医治方案分两组,对照组行阿奇霉素抗炎医治,观察组行脾氨肽口服冻干粉医治,比较两组临床效果和C反应蛋白.结果:两组临床效果比较差异显著具统计意义(P<0.05);观察组医治后IgA、IgG与T淋巴细胞亚群改善效果均比对照组优,比较差异具有统计学意义(P<0.05),且C反应蛋白、白细胞与肌酐水平均比对照组低,差异具有统计学意义(P<0.05).结论:支原体感染肺炎伴哮喘患儿行

  12. Effect of thymopetidum in refractory mycoplasma pneumonia and influence on immune function%胸腺五肽治疗难治性肺炎支原体肺炎临床疗效及对免疫状态的影响

    Institute of Scientific and Technical Information of China (English)

    陈欣; 赵涛; 郭建光; 孙文娟; 毕长柏

    2015-01-01

    ABSTRACT:Objective To observe thymopentin in adjuvant therapy for refractory mycoplasma pneumonia and influence on the immune function in children,with a view to find a safe and effective clinical treatment.Methods The study involved 1 10 cases of children with refractory mycoplasma pneumonia who were randomly divided into observation group and control group ,55 cases in each group.Control group received sequential treatment of macrolide antibiotics,while observation group was given thymopetidum on the basis of conventional treatment.The improvement of clinical symptoms was observed,the T cell subsets and immunoglobulin levels and adverse reactions were detected. Results Compared with control group,treatment group showed significantly decrease in cough time,breathing spell time,body temperature returned to normal,pulmonary rales time and chest X-ray shadow time(P 0.05).The two groups of children did not showed significant adverse reactions.Conclusion Thymopentin in adjuvant treatment of refractory mycoplasma pneumonia of children can significantly enhance immune function, improve clinical outcomes,which is worthy of clinical popularization.%目的:观察胸腺五肽辅助治疗难治性肺炎支原体肺炎的临床疗效及对机体免疫功能的影响,以期为临床寻找一种安全有效的治疗方案。方法选取110例难治性肺炎支原体肺炎患儿为研究对象,随机分为观察组和对照组各55例,对照组给予大环内酯类抗生素序贯治疗,观察组在此基础上给予胸腺五肽治疗,观察患儿临床症状改善情况,检测 T 细胞亚群和免疫球蛋白水平及不良反应。结果与对照组比较,治疗组患儿在咳嗽消失时间、喘息消失时间、体温恢复正常时间、肺部啰音消失时间和胸部 X 线阴影消失时间明显降低(均 P 0.05),两组患儿均未发生明显的不良反应。结论胸腺五肽辅助治疗难治性肺炎支原体肺炎可显著增强患儿免疫功能,提高临床

  13. Aspiration pneumonia

    Science.gov (United States)

    Anaerobic pneumonia; Aspiration of vomitus; Necrotizing pneumonia; Aspiration pneumonitis ... The type of bacteria that caused the pneumonia depends on: Your ... facility, for example) Whether you were recently hospitalized ...

  14. Comparison of culture and a multiplex probe PCR for identifying Mycoplasma species in bovine milk, semen and swab samples

    OpenAIRE

    Parker, Alysia M.; John K. House; Hazelton, Mark S.; Bosward, Katrina L.; Sheehy, Paul A.

    2017-01-01

    Mycoplasma spp. are a major cause of mastitis, arthritis and pneumonia in cattle, and have been associated with reproductive disorders in cows. While culture is the traditional method of identification the use of PCR has become more common. Several investigators have developed PCR protocols to detect M. bovis in milk, yet few studies have evaluated other sample types or other important Mycoplasma species. Therefore the objective of this study was to develop a multiplex PCR assay to detect M. ...

  15. Mycoplasma infections of plants.

    Science.gov (United States)

    Bove, J M

    1981-07-01

    Plants can be infected by two types of wall-less procaryotes, spiroplasmas and mycoplasma-like organisms (MLO), both located intracellularly in the phloem tissues of affected plants. Spiroplasmas have been cultured, characterized and shown to be true members of the class Mollicutes. MLO have not yet been cultured or characterized; they are thought to be mycoplasma-like on the basis of their ultrastructure as seen in situ, their sensitivity to tetracycline and resistance to penicillin. Mycoplasmas can also be found on the surface of plants. These extracellularly located organisms are members of the following genera: Spiroplasma. Mycoplasma and Acholeplasma. The presence of such surface mycoplasmas must not be overlooked when attempts to culture MLO from affected plants are undertaken. Sensitive serological techniques such as the enzyme-linked immunosorbent assay (ELISA) can successfully be used to compare the MLO located in the phloem of affected plants with those eventually cultured from the same plants. In California and Morocco periwinkles naturally infected with both Spiroplasma citri and MLO have been reported. With such doubly infected plants, the symptom expression has been that characteristic of the MLO disease (phyllody or stolbur), not that given by S. citri. Only S. citri can be cultured from such plants, but this does not indicate that S. citri is the causal agent of the disease expressed by the plant. In California many nonrutaceous plants have been found to be infected with S. citri. Stubborn affected citrus trees represent an important reservoir of S. citri, and Circulifer tenellus is an active leafhopper vector of S. citri. Hence, it is not surprising that in California MLO-infected fruit trees could also become infected with S. citri but it would not mean that S. citri is the causal agent of the disease. Criteria are discussed that are helpful in distinguishing between MLO infections and S. citri infections.

  16. Investigation of Chlamydophila spp. in dairy cows with reproductive disorders

    Directory of Open Access Journals (Sweden)

    Niskanen Rauni

    2008-09-01

    Full Text Available Abstract Background Reports worldwide indicate high prevalence of Chlamydophila spp. infection in cattle. To assess the prevalence in Sweden, 525 cows in 70 dairy herds with reproductive disorders was investigated. Methods To detect antibodies two commercially available kits were used. Moreover, 107 specimens, including vaginal swabs, organ tissues and milk were analysed by Polymerase Chain Reaction (PCR. Results Two (0.4% cows were seropositive in the Pourquier Cp. abortus ELISA. The seroprevalence with the Chekit ELISA was 28% with no difference between cases and controls. Five specimens were positive in real-time PCR and further analysed by nested PCR. Cp. pecorum was confirmed by partial omp1 DNA sequencing of the nested PCR product of vaginal swabs from control cows. Conclusion The results suggest that Cp. abortus infection is absent or rare in Swedish cows whereas Cp. pecorum is probably more spread. They also suggest that Chlamydophila spp. are not related to reproduction disorders in Swedish cattle.

  17. Genital Mycoplasmas in Placental Infections

    Directory of Open Access Journals (Sweden)

    Andreas Stein

    1994-01-01

    Full Text Available Objective: The involvement of the genital mycoplasmas Ureaplasma urealyticum and Mycoplasma hominis in complications of pregnancy has remained controversial especially because these microorganisms are frequent colonizers of the lower genital tract. Recovery of bacteria from the placenta appears to be the sole technique to represent a true infection and not vaginal contamination. Therefore, we investigated the presence of genital mycoplasmas, aerobic and anaerobic bacteria, and fungi in human placentas and evaluated their association with morbidity and mortality of pregnancy.

  18. 肺炎支原体肺炎患儿补体及免疫球蛋白检测及临床分析%Measurement of immunogiobulins and complements in children with Mycoplasmal pneumoniae pneumonia

    Institute of Scientific and Technical Information of China (English)

    胡学亭; 李玉娟; 李晓营; 刘瑞文

    2009-01-01

    @@ 肺炎支原体(Mycoplasma pneumoniae,MP)所致的支原体肺炎是儿科常见的呼吸道感染性疾病.近年来肺炎支原体肺炎(Mycoplasmal pneumoniae pneumonia,MPP)发病率呈明显增加趋势.

  19. Multilocus sequence typing of Mycoplasma bovis reveals host-specific genotypes in cattle versus bison

    Science.gov (United States)

    Mycoplasma bovis is a primary agent of mastitis, pneumonia and arthritis in cattle and is the bacterium isolated most frequently from the polymicrobial syndrome known as bovine respiratory disease complex (BRDC). Recently, M. bovis has emerged as a significant health problem in bison, causing necro...

  20. A multilocus sequence typing method and curated database for Mycoplasma bovis

    Science.gov (United States)

    Mycoplasma bovis is a primary agent of mastitis, pneumonia and arthritis in cattle and is the bacterium isolated most frequently from the polymicrobial syndrome known as bovine respiratory disease complex (BRDC). Recently, M. bovis has emerged as a significant problem in bison, causing necrotic pha...

  1. Molecular epidemiological analysis of Mycoplasma bovis isolates from the United Kingdom shows two genetically distinct clusters

    DEFF Research Database (Denmark)

    McAuliffe, Laura; Kokotovic, Branko; Ayling, Roger D.;

    2004-01-01

    Mycoplasma bovis is an important veterinary pathogen causing pneumonia, arthritis, and mastitis in infected cattle. We investigated the genetic diversity of 53 isolates collected in the United Kingdom between 1996 and 2002 with pulsed-field gel electrophoresis (PFGE), amplified fragment length...

  2. Relative virulence in bison and cattle of bison-associated genotypes of Mycoplasma bovis

    Science.gov (United States)

    Background. Mycoplasma bovis is a cause of respiratory disease in cattle and the bacterium most frequently isolated from bovine respiratory disease complex. It has recently emerged as a major health problem in bison, causing pharyngitis, pneumonia, arthritis, dystocia and abortion. In cattle, M. b...

  3. Abortion associated with Mycoplasma bovis (M. bovis) in a bison (Bison bison) herd

    Science.gov (United States)

    Mycoplasma bovis (M. bovis) has recently emerged as a significant health threat in bison and is an increasing concern and source of economic loss for producers. Clinical manifestations of infection documented in bison include pneumonia, respiratory distress and polyarthritis. The current study des...

  4. Clinical Application of Serum IL-8、IL-10 and PCT in Children with Mycoplasma Pneumonia%血清IL-8、IL-10、PCT在小儿肺炎支原体肺炎治疗中的临床应用

    Institute of Scientific and Technical Information of China (English)

    张虹; 顾猛

    2014-01-01

    Objective To explore the clinical application of serum IL-8、IL-10 and PCT in children with Mycoplasma Pneumonia(MPP).Methods The levels of serum IL-8、IL-10 and PCT of 62 cases of children with MPP、50 health students as control group were detected by ELISA or chemiluminescence assay at the same time.The clinical relative factors and experimental data were statistical y analyzed using SPSS18.0 statistical software,each set of experimental data expressed as mean±standard deviation,in each experimental group were analyzed by using t-test, 0.05)。结论 MPP患儿入院治疗前、治疗后血清IL-8、IL-l0,PCT含量的变化对疾病的进展和疗效有一定指导意义,提示IL-8、IL-10和PCT在MPP发病机制中起重要作用。

  5. Chlamydophila psittaci and Chlamydophila pecorum infections in goats and sheep in Egypt.

    Science.gov (United States)

    Osman, K M; Ali, H A; ElJakee, J A; Galal, H M

    2011-12-01

    The aim of this study was to investigate the epidemiology of chlamydiosis in free-ranging asymptomatic and diarrhoeic sheep and goats in Egypt. Faecal swabs were examined for the presence of Chlamydiae by culture in Vero cells and chick embryos, and staining with Giménez, direct fluorescein-conjugated monoclonal antibodies, and immunoperoxidase. Specific chlamydial DNA was identified by amplification of the omp2 gene. The asymptomatic goats were 50% positive for the presence of the omp2 gene of the family Chlamydiaceae, and all isolates were Chlamydophila psittaci. The percentage of diseased goats in which Chlamydiaceae were identified was 16.2%, and all were positive for Cp. psittaci. Of the asymptomatic sheep, 6.7% were positive for the omp2 gene of the family Chlamydiaceae, and again all were positive for Cp. psittaci. In contrast, 42.9% of the samples that were collected from the diseased sheep were positive for Chlamydiaceae, of which 25.7% were Cp. psittaci and 4.8% Cp. pecorum.

  6. New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples.

    Science.gov (United States)

    Pantchev, Alexandra; Sting, Reinhard; Bauerfeind, Rolf; Tyczka, Judith; Sachse, Konrad

    2009-08-01

    Chlamydophila psittaci and Chlamydophila abortus are the causative agents of avian chlamydiosis (psittacosis) and ovine enzootic abortion, respectively. Both pathogens are known to possess zoonotic potential. Due to their close genetic relatedness, direct and rapid species identification is difficult. In the present study, new real-time PCR assays are reported for both species. The tests are based on highly specific probes targeting the ompA gene region and were conducted as duplex PCRs including an internal amplification control. The Cp. psittaci assay successfully passed a proficiency test at national level. Examination of field samples revealed Cp. psittaci as the dominating species in birds, but also Cp. abortus in a few psittacines. Real-time PCR assays for species-specific detection of Cp. psittaci and Cp. abortus are suited for routine diagnosis, which renders them important tools for the recognition of outbreaks of psittacosis and ovine enzootic abortion.

  7. 重症肺炎支原体肺炎患儿临床特征与儿童危重病例评分的相关性分析%Analysis of correlations between clinical characteristics and pediatric critical illness score in children with severe Mycoplasma pneumoniae pneumonia

    Institute of Scientific and Technical Information of China (English)

    王程毅; 王世彪; 刘光华; 宋朝敏; 陈钦

    2016-01-01

    目的:探讨重症肺炎支原体肺炎( MPP)患儿临床特征与儿童危重病例评分( PICS)的相关性。方法纳入2014年1月至2015年12月福建省妇幼保健院PICU住院的重症MPP患儿,根据PICS分为极危重组(80分),从病史中截取年龄、性别、发热时间,入院48 h内实验室检查指标,ECG和胸部X线和CT描述。对上述指标行3组间的单因素分析,对有统计学意义的指标进一步行多因素Logistic回归分析,分析其临床特征与PICS的相关性。结果114例重症MPP患儿进入本文分析,男61例,女53例。合并心血管系统损害29例,消化系统损害23例,神经系统损害18例,血液系统损害27例。非危重组77例,危重组30例,极危重组7例,3组年龄、性别构成差异无统计学意义。3组异常ECG比例、合并2个及以上系统损害比例、合并2种及以上病原感染、病程>12 d比例、前白蛋白、D-二聚体水平差异均有统计学意义。多因素分析显示,合并2种及以上病原感染比例( OR=7.147,95%CI:1.435~35.59)、D-二聚体水平(OR=1.507,95%CI:1.054~2.156)与 PICS 呈正相关,前白蛋白水平与 PICS 呈负相关(OR =0.914,95%CI:0.845~0.990)。结论儿童重症MPP合并2种及以上病原感染、D-二聚体、前白蛋白水平和PICS相关。%Objective To investigate the relationship between clinical characteristics and pediatric critical illness score in children with severe Mycoplasma pneumoniae pneumonia( MPP). Methods Retrospective analysis was utilized for the clinical data (age,sex,duration of fever,laboratory examination,outcome of ECG,chest X-ray and CT within 48 h after admission)of the children who were hospitalized at PICU of Fujian Maternity and Children Health Hospital due to severe MPP from January 2014 to December 2015. According to the pediatric critical illness score(PCIS)criteria,patients were divided into extremely critical group

  8. PeripheralIL-10/IL-17 expression and pulmonary function in children withMycoplasma pneumoniae pneumonia%肺炎支原体肺炎患儿外周血IL-10/IL-17表达与肺功能变化的相关性研究

    Institute of Scientific and Technical Information of China (English)

    梁粤

    2015-01-01

    ObjectiveTo evaluate the correlation of peripheral IL-10/IL-17 expression and pulmonary function in children withMycoplasma pneumoniae pneumonia (MPP).MethodsChildren with MPP (n=66) were recruited and divided into MPP gasp groups (n=31) and MPP no-gasp group (n=35) based on whether pulmonary wheezing sound exists. Another 30 healthy children were recruited as control group. ELISA was used to detect the level of interleukin - 6 (IL - 6) and transforming growth factor-β (TGF-β). The peak expiratory lfow (PEF), forced expiratory volume in 1 s (FEV1), forced expiratory volume in 1 s/forced vital capacity (FEV1/FVC) and lung capacity maximum expiratory lfow (V75, V50, V25)were detected by pulmonary function instrument.ResultsThe serum IL-10 level of MPP gasp groups, MPP no-gasp group and control group were(8.1±1.8) ng/L,(13.3±2.0)ng/L and(18.4±1.7)ng/L, respectively; the serum IL-17 level were(23.8±2.3)ng/L,(16.2±1.5)ng/L and (11.4±1.0)ng/L, respectively; which showed signiifcant differences between each group(P<0.05). The PEF, FEV1 and FEV1/FVC of MPP gasp groups were(71.4±5.7)%,(71.8±6.3)% and 72.5±9.6; and those of MPP no-gasp group were(95.3±7.8)%, (96.2±10.5)% and 85.3±4.5; which showed signiifcant differences between the two groups(P<0.05). The V75, V50 and V25 were(74.7±5.0)%,(45.2±10.3)% and(38.3±8.7)%, and those of MPP no-gasp group were(85.6±4.2)%,(75.9±9.5)% and (66.1±12.5)%. Pearson correlation analysis showed that PEF, FEV1, V75, V50 and V25 had positive correlation(P˂0.05)with the level of IL-10 and f IL-17 (bothP˂0.05).ConclusionMPP children have IL-10/IL-17 imbalance, and the expression of IL-10/IL-17 was correlated with pulmonary function changes.%目的:探讨肺炎支原体肺炎(MPP)患儿外周血IL-10/IL-17表达与肺功能变化的相关性。方法选取66例MPP患儿,依据是否闻及肺部哮鸣音分为MPP喘息组(n=31)和MPP非喘息组(n=35),选取健康体检儿童30例为对照

  9. General N-and O-Linked Glycosylation of Lipoproteins in Mycoplasmas and Role of Exogenous Oligosaccharide.

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    James M Daubenspeck

    Full Text Available The lack of a cell wall, flagella, fimbria, and other extracellular appendages and the possession of only a single membrane render the mycoplasmas structurally simplistic and ideal model organisms for the study of glycoconjugates. Most species have genomes of about 800 kb and code for few proteins predicted to have a role in glycobiology. The murine pathogens Mycoplasma arthritidis and Mycoplasma pulmonis have only a single gene annotated as coding for a glycosyltransferase but synthesize glycolipid, polysaccharide and glycoproteins. Previously, it was shown that M. arthritidis glycosylated surface lipoproteins through O-linkage. In the current study, O-linked glycoproteins were similarly found in M. pulmonis and both species of mycoplasma were found to also possess N-linked glycans at residues of asparagine and glutamine. Protein glycosylation occurred at numerous sites on surface-exposed lipoproteins with no apparent amino acid sequence specificity. The lipoproteins of Mycoplasma pneumoniae also are glycosylated. Glycosylation was dependent on the glycosidic linkages from host oligosaccharides. As far as we are aware, N-linked glycoproteins have not been previously described in Gram-positive bacteria, the organisms to which the mycoplasmas are phylogenetically related. The findings indicate that the mycoplasma cell surface is heavily glycosylated with implications for the modulation of mycoplasma-host interactions.

  10. Mycoplasma bovis infections and co-infections with other Mycoplasma spp. with different clinical manifestations in affected cattle herds in eastern region of Poland

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    Szacawa Ewelina

    2015-09-01

    Full Text Available The aim of the study was to evaluate the presence of Mycoplasma bovis infection and co-infections with other Mycoplasma spp. infections in cattle. The tested population was one in the eastern region of Poland containing 66 dairy cows and 23 calves showing different clinical signs and suffering from pneumonia, mastitis, and arthritis. The incidence of M. bovis in co-infections with other Mycoplasma spp. was examined using serological traditional mycoplasma culture methods, and the molecular methods - PCR and polymerase chain reaction/denaturing gradient gel electrophoresis (PCR/DGGE. The PCR/DGGE method for detecting Mycoplasma spp. in cattle was used for the first time in Poland. The seroprevalence of M. bovis in the affected cattle herds in the eastern region of Poland was 47.8% in calves and 19.7% in dairy cows. The direct detection and identification of M. bovis from nasopharyngeal swabs by PCR revealed that 56.5% of calves were positive, but all of the dairy cows were negative. The PCR/DGGE identified eight (34.8% instances of M. arginini and eight (26.1% instances of M. bovirhinis co-infecting with M. bovis in ten calves. The seroprevalence of M. bovis in the tested population was 33.7%. Any future attempts to control mycoplasma infections require an insight into the current epidemiological situation of M. bovis infection and its relationship to other mycoplasmas in causing clinical disease in cattle. Using these diagnostic methods we have demonstrated that mycoplasmal infections are often caused by multiple species of Mycoplasma and not just the primary M. bovis pathogen.

  11. OmpA and antigenic diversity of bovine Chlamydophila pecorum strains.

    Science.gov (United States)

    Kaltenboeck, B; Heinen, E; Schneider, R; Wittenbrink, M M; Schmeer, N

    2009-03-16

    Infections with the intracellular bacterium Chlamydophila (C.) pecorum are highly prevalent worldwide in cattle. These infections cause significant diseases such as polyarthritis, pneumonia, enteritis, genital infections and fertility disorders, and occasionally sporadic bovine encephalomyelitis. Subclinical respiratory infections of calves with C. pecorum have been associated with airway obstruction, pulmonary inflammation, and reduced weight gains. This investigation examined four chlamydial strains with biological properties of C. pecorum isolated from feces of clinically normal cattle, from calves with pneumonia, and from bulls with posthitis. The objective was to characterize the evolutionary relationships of these bovine chlamydial isolates to other chlamydiae by genetic analysis of the ompA gene, and by the immunological cross-reactivities in Western immunoblot analysis. PCR typing of the ompA gene identified these isolates as C. pecorum. The OmpA-deduced amino acid dissimilarities between these four strains spanned 10-20%. In phylogenetic analysis, the four isolates clustered with C. pecorum ruminant, porcine, and koala strains of different geographic origins rather than with each other. All four isolates showed different patterns of Western immunoblot reactivity with antiserum against bovine C. pecorum strain LW63, and, interestingly, no cross-reactivity of the OmpA proteins with the anti-LW613 OmpA antibodies. These data underscore the polyphyletic population structure of C. pecorum and suggest that the spectrum of C. pecorum OmpA proteins in a host species can occupy the entire evolutionary bandwidth within C. pecorum. The variant immunoblot reactivities support the notion of considerable genomic plasticity of C. pecorum.

  12. Preparation of Yoly against Mycoplasma Bovis and Pasteurella Multocida Pneumoniae and Preliminary Observation on Clinical Application%抗牛支原体及多杀性巴氏杆菌二联卵黄抗体的制备及初步临床应用观察

    Institute of Scientific and Technical Information of China (English)

    高家登; 杨铭伟; 剡根强; 王静梅; 王超丽; 杨龙龙

    2014-01-01

    本研究将自制牛支原体和牛源荚膜血清A型多杀性巴氏杆菌灭活菌苗分别免疫产蛋鸡,并检测效价,采用卡拉胶结合硫酸铵沉淀法提取IgY,观察抗牛支原体及多杀性巴氏杆菌二联高免卵黄抗体制剂在犊牛中应用的效果,并进行临床应用研究。结果显示,牛支原体在首免后第60d抗体效价达到高峰,经ELISA检测效价为1:128000;牛多杀性巴氏杆菌首免后第50d抗体效价达到高峰,经IHA检测效价为1:1024。临床初步应用表明,二联卵黄抗体对犊牛肺炎的预防和治疗均有一定作用。%To prepare of yoly against Mycoplasma bovis and Pasteurella multocida pneumoniae and observe the effect on clinical application.Through self-made inactivated M.bovis and bovine serum capsular type A Pasteurella multocida vaccine were immunized hens, and detect the titer, the method of carrageenan combined with ammonium sulfate to extract IgY, the antibody formulations applied to the calf and observe the effect. Finally to do clinical study. Results showed that M.bovis yolk antibody titer reached the highest at the 60th day after ifrst immunization and Yolk antibody titers 1:128000, which was detected by the method of indirect ELISA, Pasteurella multocida pneumoniae yolk antibody titer reached the highest at the 50th day after ifrst immunization and Yolk antibody titers 1:1024 ,was detected by the method of IHA. Preliminary clinical application showed that the duplex yolk antibodies have effect to calf pneumonia on prevention and treatment .

  13. Comparision of clinical diagnostic value between PCR and TaqMan RT-PCR for Mycoplasma pneumoniae in throat swabs%PCR及RT-PCR检测咽拭子标本肺炎支原体的比较

    Institute of Scientific and Technical Information of China (English)

    李淳; 吴移谋; 朱翠明; 钟礼立; 陈丹; 吕建华

    2012-01-01

    To compare the clinical diagnostic value between PCR and RT PCR for M. Pneumoniae in swab, we per formed both PCR and RT PCR assays analysis for M. Pneumoniae DNA on a total of 566 samples of throat swab from 106 ped iatric children with M. Pneumoniae and in whom M. Pneumoniae was suspected. Among the 566 pediatric children, there were 45(7. 95%) PCR positive specimens and 175(30. 92%) RT PCR positive specimens. In the 106 pediatric children with M. Pneumoniae, 5 were positive for PCR, and 95 were positive for RT PCR. In the 460 pediatric children with symptom of M. Pneumoniae, 40 were positive for PCR, and 80 were positive for RT PCR. The sensitivy of Rt PCR for M. Pneumoniae detec tion appeared to be better than that of PCR. (sensitivity . RT PCR 89. 62 % , PCR 4. 72 % , x2=146. 322, P = 0. 000), but there was no significant difference in the specificity between RT PCR and PCR (specifitivity: RT PCR 82. 60 %, PCR 91. 30%,x2 - 3. 331, P - 0. 068). It is concluded that the TaqMan based RT PCR assay is a rapid, sensitive and specific meth od for the detection of M. Pneumoniae in throat swabs of children in early period of diagnosis.%目的 应用聚合酶链反应(PCR)与实时taqMan荧光定量PCR(RT-PCR)检测咽拭子标本中的肺炎支原体DNA(Mp-DNA),比较2种方法检测结果的临床诊断价值.方法 随机选取I临床儿科门诊患儿566例,包括临床治诊Mp感染患儿106例和临床疑似Mp感染忠儿460例,分别采用PCR法和RT-PCR法检测,以临床治诊Mp作为参照标准,采用x2检验评定2种检测方法诊断的灵敏度和特异度,比较2种检测方法对Mp的诊断价值.结果 566份受检患儿的咽拭子标本中,PCR法检测阳性45例(7.95%)(临床治诊Mp感染患儿5例,临床疑似Mp感染患儿40例),RT-PCR法检测阳性175例(30.92%)(临床治诊Mp感染患儿95例,临床疑似Mp感染患儿80例).RT-PCR法检测咽拭子Mp-DNA诊断Mp感染的敏感度显著高于PCR法(敏感度RT-PCR 89.62%,PCR 4.72%,x2=146.322,P

  14. The Phospholipid Profile of Mycoplasmas

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    Jonathan D. Kornspan

    2012-01-01

    Full Text Available The de novo synthesized polar lipids of Mycoplasma species are rather simple, comprising primarily of the acidic glycerophospholipids PG and CL. In addition, when grown in a medium containing serum, significant amounts of PC and SPM are incorporated into the mycoplasma cell membrane although these lipids are very uncommon in wall-covered bacteria. The exogenous lipids are either incorporated unchanged or the PC incorporated is modified by a deacylation-acylation enzymatic cycle to form disaturated PC. Although their small genome, in some Mycoplasma species, other genes involved in lipid biosynthesis were detected, resulting in the synthesis of a variety of glycolipis, phosphoglycolipids and ether lipids. We suggest that analyses and comparisons of mycoplasma polar lipids may serve as a novel and useful tool for classification. Nonetheless, to evaluate the importance of polar lipids in mycoplasma, further systematic and extensive studies on more Mycoplasma species are needed. While studies are needed to elucidate the role of lipids in the mechanisms governing the interaction of mycoplasmas with host eukaryotic cells, the finding that a terminal phosphocholine containing glycolipids of M. fermentans serves both as a major immune determinants and as a trigger of the inflammatory responses, and the findings that the fusogenicity of M. fermentans with host cells is markedly stimulated by lyso-ether lipids, are important steps toward understanding the molecular mechanisms of M. fermentans pathogenicity.

  15. An investigation into the role of Chlamydophila spp. in bovine upper respiratory tract disease.

    Science.gov (United States)

    Twomey, D F; Griffiths, P C; Horigan, M W; Hignett, B C; Martin, T P

    2006-05-01

    An outbreak of upper respiratory tract disease was investigated in a group of 17 housed home-bred calves on a mixed dairy, beef and sheep farm in Devon. Conjunctival swabs were collected and tested for Chlamydophila spp. DNA using a PCR test that detects Chlamydophila abortus and Chlamydophila psittaci. Six of the calves tested gave a positive result. Further epidemiological observations and laboratory testing indicated that the adult dairy cows, from which the affected calves originated, were the most likely source of infection.

  16. Expression of human proinflammatory cytokines from monocytic cells induced by Cpn0425 recombinant protein from Chlamydophila pneumoniae and apoptosis%Cpn0425重组蛋白诱导细胞凋亡和产生前炎症因子的研究

    Institute of Scientific and Technical Information of China (English)

    肖光文; 刘良专; 谢小平; 吴移谋

    2013-01-01

    stimulated by different concentrations of the recombinant protein of Cpn0425 from Chlamydophila pneumonioe after sonication,after removal of endotoxin by purification columns of ToxinEraser;,the expression of IL-8 and IL-1β were tested and proirferation by ELISA.Zhe inhibition of cell treated with Cpn0425 was assessed by WST-1.Cell apoptosis was detected by Annexin-V-FITC-PI.Results The recombinant protein of Cpn0425 stimulated THP-1 cell expressing the mRNA of IL-8 and IL-1 β and produce proinflamatory cytokines including IL-8 and IL-1 β in a dose and time-dependent manner,Cell proliferation of THP-1 was inhibited in a dose-dependent manner of GST-Cpn0425;The apoptosis was induced 24 hours after treatment of THP-1 cells with GST-Cpn0425,its highest apoptosis rate reached 17.76 ± 4.2%.Conclusions The recombinant protein of Cpn0425 can induce the expression of THP-1 cells and secretion of proinflammatory cytokines IL-8 and IL-1β,inhibit the proliferation and apoptosis of THP-1 cells;therefore may be an important virulence factor.

  17. In vitro activities of demestic macrolides against Chlamydia trachomatis and Chlamydophila pneumoniae%四种国产大环内酯类抗菌药物体外抗沙眼衣原体和肺炎衣原体敏感性研究

    Institute of Scientific and Technical Information of China (English)

    倪安平; 崔京涛; 汪晓巍

    2008-01-01

    目的 研究国产必特螺旋霉素、乙酰螺旋霉素、红霉素和阿奇霉素等4种大环内酯类药物对于沙眼衣原体和肺炎衣原体体外药物敏感性试验,评估其抗衣原体作用,以指导临床用药.方法 细胞培养和免疫荧光包涵体染色技术测定4种国产大环内酯类抗菌药物对于沙眼衣原体和肺炎衣原体体外MIC.结果 对于沙眼血清型B,必特螺旋霉素、红霉素和阿奇霉素体外MIC为0.5μg/ml,乙酰螺旋霉素为4μg/ml.对于沙眼血清型D,必特螺旋霉素与阿奇霉素体外MIC均为0.25μg/ml,红霉素0.5μg/ml,乙酰螺旋霉素2μg/ml.对于肺炎衣原体,红霉素体外MIC≤0.016μg/ml,阿奇霉素和必特螺旋霉素均为0.032μg/ml,乙酰螺旋霉素0.5μg/ml.结论 国产必特螺旋霉素、红霉素和阿奇霉素体外抗沙眼衣原体(血清型B和D)和肺炎衣原体作用可靠,但乙酰螺旋霉素则较差.%Objective To evaluate the activities of four demestic macrolides against C. trachomatis and C. pneumoniae by antimicrobiai susceptibility testing. Methods Cell culture and immunoflourescence staining of chlamydial inclusions were used to determine MICs of four demestic macrolides against C. trachomatis and C. pneumoniae. Results MIC (0.5 μg/ml) was found for acylspriramycin,erythromycin and azithromycin against C. trachomatis serovar B while it was 4 μg/ml for acetylspiramycin. Agaisnt C. trachomatis serovar D, MIC was 0.25 μg/mi in both acylspriramycin and azithromycin, and MICs were 0.5 μg/ml and 2 μml in erythromycin and acetylspiramycin, separately. Agaisnt C. pneumoniae TWAR, erythromycin was the most active with MIC≤0. 016 μg/ml, acylspriramycin and azithromycin were the second with same M1C of 0.032 μg/ml. However, acetylspiramycin was less active with 0.5 μg/ml of MIC. Conclusion Except acetylspiramycin, acylspriramycin erythromycin and azithromycin had reliable activities against both C. trachomatis (serovar B and D) and C. pneumoniae.

  18. Chlamydophila psittaci infection of birds and humans

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    Bülent Baş

    2015-04-01

    seen in infected birds. Transmission of infection to humans occurs through inhalation or direct contact and transmission through bird bites or human-to-human is rare. C. psittaci usually leads to the systemic infection associated with pneumonia in humans. In recent years, PCR based molecular methods are used as well as serological methods such as ELISA, CFT, MIF in diagnosis. Both of infected birds and humans, tetracyclines and macrolides are preferred for treatment of infection. In order to prevent the disease, due to there isn't any commercial vaccine for especially using in birds, applying biosafety rules is very important in terms of human health and economical aspects. Especially, veterinarians, bird breeders and dealers, poultry farmers and slaughterhouse workers are at high risk for C. psittaci infection. Due to the transmission to humans of psittacosis infection and accepting it as a potential biological weapon, it is thought to be important for public health. In this review, it is aimed to give detailed information about infection in human and birds, because it can be missed at the diagnosis, hence there is low awareness about disease and it has got variable clinical symptoms.

  19. Correlation of Chlamydia and Chlamydophila spp. IgG and IgM antibodies by microimmunofluorescence with antigen detection methods.

    Science.gov (United States)

    She, Rosemary C; Welch, Ryan; Wilson, Andrew R; Davis, David; Litwin, Christine M

    2011-01-01

    Correlation of serologic titers for Chlamydia trachomatis with other tests has been based on direct fluorescence antibody (DFA) testing and culture, but not on nucleic acid-based tests that are used for screening. We retrospectively reviewed the specificity of antibodies against C. trachomatis, Chlamydia psittaci, and Chlamydophila pneumoniae by microimmunofluorescence (MIF) when compared with DFA, culture, nucleic acid probe, and transcription-mediated amplification. Over a 6-year period, 226 cases had both MIF and one of these other methods performed for comparison. Agreement between C. trachomatis antigen or nucleic acid detection and MIF results was 87% (197/226). C. trachomatis serology had a negative predictive value of 98%, and 10.6% of cases were positive by serology and negative by antigen testing. Of the 13 patients who had a positive C. trachomatis antigen or nucleic acid test result, 9 had IgG and/or IgM titers highest against C. trachomatis, 3 had IgG titers highest against C. pneumoniae, and 1 had undetectable titers for the three chlamydial species. Twenty-five patients had positive IgG and/or IgM titers to C. trachomatis but negative antigen test results. Serologic testing can increase the sensitivity of detecting C. trachomatis infections.

  20. Curative effect of spleen ammonia peptide oral freeze-dried power on treatment of children pneumonia mycoplasma infection and influence on immunological indexes%脾氨肽口服冻干粉治疗小儿肺炎支原体感染的疗效及对免疫学指标的影响

    Institute of Scientific and Technical Information of China (English)

    李孔珍; 李孔英

    2016-01-01

    Objective To analyze the curative effect of spleen ammonia peptide oral freeze-dried power on treatment of children pneumonia mycoplasma infection and its influence on immunological indexes .Methods From July 2012 to July 2014 100 cases of pneumonia mycoplasma infection admitted in Longquan People ’ s Hospital were selected as research objects , and they were divided into convention group and spleen ammonia peptide group according to random number table method with 50 cases.The convention group was given fundamental anti-infection and anti -viral treatment , while the spleen ammonia peptide group was provided with spleen peptide oral ammonia based on foundation treatment .The clinical curative effect , symptoms disappear ance time , incidence of respiratory tract infection and immunological indexes changes were compared between two groups .Results In spleen ammonia peptide group , the total effective rate increased but incidence of respiratory tract infection decreased obviously compared with the conventional group (χ2 value was 4.35 and 4.45, respectively, both P<0.05).The symptoms disappear ance time was significantly shorter in spleen ammoniac peptide group than that in the conventional group (t =2.89, P <0.05).In the conventional group, IgM, IgA and C4 levels after treatment increased significantly than those before treatment (t value was 2.34, 3.11 and 3.45, respectively, all P<0.05).In Spleen ammonia peptide group, IgM, IgA and C4 levels after treatment were more significantly higher than before treatment ( t value was 2.93, 3.67 and 4.03, respectively, all P<0.05).After treatment, IgM, IgA and C4 level in spleen ammonia peptide group increased more obviously than in the conventional group (t value was 3.25, 3.43 and 3.56, respectively, all P<0.05).Conclusion Foundation treatment combined with spleen ammonia peptide oral freeze -dried powder for treatment of children pneumonia mycoplasma infection is worthy of popularization and application for significant