WorldWideScience

Sample records for mo nj ny

  1. NATURAL ATTENUATION FOR ECOSYSTEM RESTORATION IN NY/NJ HARBOR

    Energy Technology Data Exchange (ETDEWEB)

    Van der Lelie, D.; Reid-Green, J. D.; Stern, E. A.

    2003-12-31

    We have investigated the feasibility of using natural attenuation methods for ecosystem restoration in New York/New Jersey Harbor. Measurements were made of the most probable number of sulfate-reducing bacteria (SRB) in native sediments and in samples, which had been supplemented with an appropriate electron donor and electron acceptor. The results showed that the activity of the endogenous microbial population in the native sediment was high enough to make possible adequate chemical transformation rates. The bioavailability of the zinc in the sediments was measured using the BIOMET biosensor technique. The bioavailability of the zinc was effectively eliminated following the microbial activities. We concluded that natural attenuation could be used effectively in treating sediments from Newark Bay and surrounding waters and that the resultant materials could likely be used in environmental restoration projects of the type proposed for construction in South Kearny, NJ.

  2. Atmospheric dry deposition of trace elements measured around the urban and industrially impacted NY-NJ harbor

    Science.gov (United States)

    Yi, Seung-Muk; Totten, Lisa A.; Thota, Sathyapriya; Yan, Shu; Offenberg, John H.; Eisenreich, Steven J.; Graney, Joseph; Holsen, Thomas M.

    Long-term direct trace metal dry deposition measurements were made during 2001 and 2002 at two sites near the NY/NJ Harbor Estuary in New Jersey: Jersey City (JC) and New Brunswick (NB). In addition intensive dry deposition and atmospheric size distribution (ASD) measurements were made between September 2001 and June 2002. Dry deposition samples were obtained using modified MIC-B wet deposition samplers which deployed knife-edge surrogate surfaces during dry periods. ASDs were measured with micro-orifice uniform deposit impactors (MOUDI) and coarse particle rotary impactors (CPRI). The fluxes of trace metals were found to be highest at the JC site and lowest at the NB site. The dry deposition fluxes ranged from 0.030 (Cd) to 3800 (Al) μg m -2 d -1 and 0.14 (Cd) to 8100 (Al) μg m -2 d -1at NB and JC sites, respectively. Metals of crustal origin were found mostly in the coarse particle fraction and anthropogenic metals were found mostly in the fine particle fraction. For the intensive experiments, overall dry deposition velocities of several trace metals were determined by dividing measured fluxes by measured concentrations in different particle size ranges. For most of the elements, the flux was highly correlated with PM 10 and coarse particle concentrations. The overall dry deposition velocities of crustal elements (Al and Mg) varied from 3.7 to 8.7 cm s -1 with correlation coefficients ranging from 0.50 to 0.84. The best-fit dry deposition velocities for anthropogenic elements (Cr, Mn, Zn, and Pb; Cu, Mo, and Ba; V and Ni) varied more than those for crustal elements (0.52-25 vs. 3.7-8.7 cm s -1). The dry deposition velocities for most elements obtained using three size ranges were statistically significant with R2 values ranging from 0.37 to 0.84 except for V and Ni (0.12).

  3. Extreme differentiation in single samples from the olivine-rich zone of the Palisades Sill, NY and NJ

    Science.gov (United States)

    Chau, K. X.; Sen, G.; Naslund, H. R.

    2007-12-01

    The Palisades Sill, NY and NJ, has long been the focus of much attention due to its potential contributions to the understanding of igneous differentiation. Many early authors, including N.L. Bowen, thought of the intrusion as an ideal example of crystal settling because of an olivine-rich zone that occurs close to the base of the sill. Later investigations demonstrated that at least three geochemically distinct batches of magma were emplaced at different levels within this body, one of which produced the olivine-rich zone. Here we report the results of our detailed electron probe study of 27 olivine grains, 134 plagioclase grains, and 181 pyroxene grains from four samples of the olivine-rich zone. In one of the rocks plagioclase ranges from An84 (large grain; core) to An0.5 (interstitial plagioclase), whereas olivine ranges from Fo81 - Fo69. These ranges are comparable to our other samples. The plagioclase range is far greater than what is shown by the Skaergaard (An25 - An69) and Bushveld (An30 - An80) intrusions. Though the sill has long been known to be vertically differentiated, such extreme differentiation in individual thumb-sized samples was not expected. The implication is that interstitial melt pockets were so well enclosed (i.e., zero permeability) by the surrounding crystals that they were sealed off from further communication with larger volumes of melt that may have existed at a higher levels in the sill at that time. That is, compaction and associated filter pressing was not efficient in removing the interstitial melt. It is unlikely that such a situation could occur if the olivine-rich zone formed early from a large body of magma because the load pressure of the overlying crystals and melt would be too great to avoid compaction. A better explanation is that the olivine-rich zone was emplaced as a separate magma batch (as suggested by several previous workers based on bulk rock geochemistry) in a mostly solidified magma-crystal mush. In this setting

  4. NOAA Office for Coastal Management Benthic Habitat Data, Long Island Sound, Jamaica Bay, and Lower Bay of NY/NJ Harbor, NY, 1994-2002 (NODC Accession 0089467)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data are a collection of benthic habitat data from studies conducted in the coastal Long Island Sound, NY region in GIS shapefile (.shp, .dbf, .shx, and .prj...

  5. Sole Source Aquifers for NY and NJ

    Data.gov (United States)

    U.S. Environmental Protection Agency — This layer is the designated sole source aquifers of New York and New Jersey. A Sole Source Aquifer, is an aquifer that supplies 50% or more of the drinking water...

  6. 2012 USACE Topobathy Lidar: Post Sandy (NJ & NY)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These files contain classified topographic and bathymetric lidar data as unclassified valid topographic data (1) and valid topographic data classified as ground (2),...

  7. 33 CFR 80.501 - Tom's River, NJ to Cape May, NJ.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Tom's River, NJ to Cape May, NJ. 80.501 Section 80.501 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Fifth District § 80.501 Tom's River, NJ to Cape May, NJ...

  8. What Is the Relative Influence of NJ School Report Card Variables on NJ ASK 5 Scores?

    Science.gov (United States)

    Gemellaro, Dorian Marrone

    2012-01-01

    In this study, the researcher examined the strength and direction of relationships between NJ School Report Card Variables (NJ SRC) and 2008-2009 NJ ASK 5 Math and Language Arts Literacy (LAL) student test scores. Variables found to have an influence on standardized test scores in the extant literature were evaluated and reported. Analyses were…

  9. 33 CFR 80.170 - Sandy Hook, NJ to Tom's River, NJ.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Sandy Hook, NJ to Tom's River, NJ. 80.170 Section 80.170 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Atlantic Coast § 80.170 Sandy Hook, NJ to Tom's River...

  10. Boosting Wigner's nj-symbols

    CERN Document Server

    Speziale, Simone

    2016-01-01

    We study the SL(2,C) Clebsch-Gordan coefficients appearing in the lorentzian EPRL spin foam amplitudes for loop quantum gravity. We show how the amplitudes decompose into SU(2) nj-symbols at the vertices and integrals over boosts at the edges. The integrals define edge amplitudes that can be evaluated analytically using and adapting results in the literature, leading to a pure state sum model formulation. This procedure introduces virtual representations which, in a manner reminiscent to virtual momenta in Feynman amplitudes, are off-shell of the simplicity constraints present in the theory, but with the integrands that peak at the on-shell values. We point out some properties of the edge amplitudes which are helpful for numerical and analytical evaluations of spin foam amplitudes, and suggest among other things a simpler model useful for calculations of certain lowest order amplitudes. As an application, we estimate the large spin scaling behaviour of the simpler model, on a closed foam with all 4-valent edg...

  11. Boosting Wigner's nj-symbols

    Science.gov (United States)

    Speziale, Simone

    2017-03-01

    We study the SL (2 ,ℂ ) Clebsch-Gordan coefficients appearing in the Lorentzian EPRL spin foam amplitudes for loop quantum gravity. We show how the amplitudes decompose into SU(2) nj- symbols at the vertices and integrals over boosts at the edges. The integrals define edge amplitudes that can be evaluated analytically using and adapting results in the literature, leading to a pure state sum model formulation. This procedure introduces virtual representations which, in a manner reminiscent of virtual momenta in Feynman amplitudes, are off-shell of the simplicity constraints present in the theory, but with the integrands that peak at the on-shell values. We point out some properties of the edge amplitudes which are helpful for numerical and analytical evaluations of spin foam amplitudes, and suggest among other things a simpler model useful for calculations of certain lowest order amplitudes. As an application, we estimate the large spin scaling behaviour of the simpler model, on a closed foam with all 4-valent edges and Euler characteristic χ , to be Nχ -5 E +V /2. The paper contains a review and an extension of the results on SL (2 ,ℂ ) Clebsch-Gordan coefficients among unitary representations of the principal series that can be useful beyond their application to quantum gravity considered here.

  12. Green infrastructure monitoring in Camden, NJ

    Science.gov (United States)

    The Camden County Municipal Utilities Authority (CCMUA) installed green infrastructure Stormwater Control Measures (SCMs) at multiple locations around the city of Camden, NJ. The SCMs include raised downspout planter boxes, rain gardens, and cisterns. The cisterns capture water ...

  13. EnviroAtlas - Paterson, NJ - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the Paterson, NJ EnviroAtlas area. The block groups are from the US Census Bureau and are included/excluded based on...

  14. Nye Medier - ny journalistik?

    DEFF Research Database (Denmark)

    Hartley, Jannie Møller

    2009-01-01

    På baggrund af en pilotundersøgelse undersøger denne artikel forskningens hypoteser om netjournalistikken, med særligt fokus på den nordiske litteratur på området. Det argumenteres, at der med netmediet og netavisernes vækst ikke nødvendigvis er tale om ny journalistik; på den ene side bekræfter ...... nogle traditionelle journalistiske rutiner i nyhedsproduktionen.En radikalisering der indikerer, at nyheden i den digitale tidsalder kan ses som en dynamisk proces frem for et statisk produkt. Udgivelsesdato: 2009...

  15. Faglighed + test = ny elevtype

    DEFF Research Database (Denmark)

    Drejer, Claus Munch

    2010-01-01

    Kronikken handler om tiden efter den seneste folkeskolelov, samt de teknikker der indføres i bestræbelserne på at optimere elevernes færdigheder og kundskaber. Siden de dårlige PISA-test i 2000, og specielt 2003, har der været fokus på det kernefaglige, og for at sikre eleverne denne faglighed in...... indføres en række teknikker såsom evaluering, test, trin- og slutmål, der skal sikre at eleverne optimere deres faglighed. Dette peger frem mod en ny elevtype - en præstationsorienteret elev....

  16. 75 FR 39839 - Regulated Navigation Area; Hudson River and Port of NY/NJ

    Science.gov (United States)

    2010-07-13

    ... restrictions with the Kiewit and Weeks Marine contractors, and with Hudson River and Sandy Hook Pilots... transfer of the bridge span from shore to the barges has been scheduled on a weekday when it is expected to... Overtaking zones are established in areas identified by Weeks Marine, Hudson River and Sandy Hook Pilots as...

  17. 76 FR 52569 - Regulated Navigation Area; Arthur Kill, NY and NJ

    Science.gov (United States)

    2011-08-23

    ... channel, require the relocation of lateral aids to navigation, and create a reduction in the width of the.... If you think that your business, organization, or governmental jurisdiction qualifies as a small... ADDRESSES) explaining why you think it qualifies and how and to what degree this rule would...

  18. 77 FR 1023 - Regulated Navigation Area; Arthur Kill, NY and NJ

    Science.gov (United States)

    2012-01-09

    .... If you submit comments by mail or hand delivery, submit them in an unbound format, no larger than 8\\1... meaning of the Administrative Procedure Act (APA), 5 U.S.C. 553. But you may submit a request for one..., under the authority of the Administrative Procedure Act (APA), 5 U.S.C. 553(b), and giving effect to it...

  19. Tendenser i ny tysk litteratur

    DEFF Research Database (Denmark)

    Povlsen, Steen Klitgård

    Arbejdspapiret indeholder to foredrag: dels om ny tysk litteraturs forhold til 2. verdenskrig, dels om Pater Handke og hans roman "Don Juan (fortalt af ham selv)......Arbejdspapiret indeholder to foredrag: dels om ny tysk litteraturs forhold til 2. verdenskrig, dels om Pater Handke og hans roman "Don Juan (fortalt af ham selv)...

  20. 78 FR 44433 - Safety Zone; Metedeconk River; Brick Township, NJ

    Science.gov (United States)

    2013-07-24

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Metedeconk River; Brick Township, NJ AGENCY... regulation applies to only one recurring fireworks event held in the Metedeconk River in Brick Township, NJ... CFR 165.506, an entry 19 for the Metedeconk River, Brick Township, NJ Safety Zone will be added from...

  1. Polymeric Structure and Host Toll-like Receptor 4 Dictate Immunogenicity of NY-ESO-1 Antigen in Vivo*

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W.; Aldridge, Michael E.; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P.; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-01-01

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern. PMID:21900253

  2. Polymeric structure and host Toll-like receptor 4 dictate immunogenicity of NY-ESO-1 antigen in vivo.

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W; Aldridge, Michael E; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-10-28

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern.

  3. Sandia and NJ TRANSIT Authority Developing Resilient Power Grid

    Energy Technology Data Exchange (ETDEWEB)

    Hanley, Charles J. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Ellis, Abraham [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2014-11-01

    Through the memorandum of understanding between the Depratment of Energy (DOE), the New Jersey Transit Authority (NJ Transit), and the New Jersey Board of Public Utilities, Sandia National Labs is assisting NJ Transit in developing NJ TransitGrid: an electric microgrid that will include a large-scale gas-fired generation facility and distributed energy resources (photovoltaics [PV], energy storage, electric vehicles, combined heat and power [CHP]) to supply reliable power during storms or other times of significant power failure. The NJ TransitGrid was awarded $410M from the Department of Transportation to develop a first-of-its-kind electric microgrid capable of supplying highly-reliable power.

  4. Studerende giver ny indsigt til digital byudvikling

    DEFF Research Database (Denmark)

    2016-01-01

    http://studerende.au.dk/studier/fagportaler/arts/aktuelt/nyheder/vis-nyhed/artikel/studerende-giver-ny-indsigt-til-digital-byudvikling/......http://studerende.au.dk/studier/fagportaler/arts/aktuelt/nyheder/vis-nyhed/artikel/studerende-giver-ny-indsigt-til-digital-byudvikling/...

  5. Udlejers godkendelse af ny erhvervslejer

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    2009-01-01

    I artiklen er taget udgangspunkt i den »klassiske« afståelsessituation, hvor en lejer vil afhænde den i de lejede lokaler drevne virksomhed, og lejeren har afståelsesret til en af udlejer godkendt lejer, uden at kravene til den ny lejer er specificeret. På baggrund af Vestre Landsrets dom af 9/2 ...

  6. Ny industrialisering - et bedre produktmarked

    DEFF Research Database (Denmark)

    Beim, Anne

    2001-01-01

    for byggeriet med titlerne: 1. Bygherren som forandringsagent. 2. Tæt samarbejde i byggedelen. 3. Ny industrialisering. 4. Byg med kommunikation og innovation. Dette debathæfte er nummer tre i rækken og omhandler byggeriets industridel og mulighederne for at fremme en succesfuld industriel produktion....

  7. 78 FR 3836 - Drawbridge Operation Regulation; Shark River, Avon, NJ

    Science.gov (United States)

    2013-01-17

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Shark River, Avon, NJ AGENCY: Coast... which are across the Shark River (South Channel), at Avon Township, NJ. This deviation is necessary to facilitate machinery replacement on the Shark River railroad bridge. This temporary deviation will allow...

  8. 78 FR 77591 - Drawbridge Operation Regulation; Shark River, NJ

    Science.gov (United States)

    2013-12-24

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Shark River, NJ AGENCY: Coast Guard... Shark River (South Channel), mile 0.8, at Belmar, NJ. The deviation is necessary to facilitate the... facilitate the replacement of motor seals and instrumentation on the bridge. The Route 71 Bridge across...

  9. 77 FR 57022 - Drawbridge Operation Regulation; Shark River, Avon, NJ

    Science.gov (United States)

    2012-09-17

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Shark River, Avon, NJ AGENCY: Coast..., and the S35 bridge, mile 0.9, all of which are across the Shark River (South Channel), at Avon Township, NJ. This deviation is necessary to facilitate stringer replacement on the Shark River...

  10. 75 FR 52463 - Safety Zone; Raccoon Creek, Bridgeport, NJ

    Science.gov (United States)

    2010-08-26

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Raccoon Creek, Bridgeport, NJ AGENCY: Coast... specified waters of Raccoon Creek, Bridgeport, NJ. This action is necessary to provide for the safety of... intended to restrict vessel access in order to protect mariners in a portion of Raccoon Creek. DATES: This...

  11. Cistern Performance for Stormwater Management in Camden, NJ - presentation

    Science.gov (United States)

    The Camden County Municipal Utilities Authority (CCMUA) installed different types of green infrastructure Stormwater Control Measures (SCMs) at locations around the city of Camden, NJ. The installed SCMs include cisterns. Cisterns provide a cost effective approach to reduce st...

  12. First year update on green infrastructure monitoring in Camden, NJ

    Science.gov (United States)

    The Camden County Municipal Utilities Authority (CCMUA) installed green infrastructure Stormwater Control Measures (SCMs) at multiple locations around the city of Camden, NJ. The SCMs include raised downspout planter boxes, rain gardens, and cisterns. The cisterns capture water ...

  13. First year results on cistern monitoring in Camden, NJ

    Science.gov (United States)

    The Camden County Municipal Utilities Authority (CCMUA) installed green infrastructure Stormwater Control Measures (SCMs) at multiple locations around the city of Camden, NJ. The SCMs include raised downspout planter boxes, rain gardens, and cisterns. The cisterns capture water ...

  14. Cistern Performance for Stormwater Management in Camden, NJ - abstract

    Science.gov (United States)

    The Camden County Municipal Utilities Authority (CCMUA) installed different types of green infrastructure Stormwater Control Measures (SCMs) at locations around the city of Camden, NJ. The installed SCMs include cisterns. Cisterns provide a cost effective approach to reduce st...

  15. EnviroAtlas - Paterson, NJ - Atlas Area Boundary

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset shows the boundary of the Paterson, NJ Atlas Area. It represents the outside edge of all the block groups included in the EnviroAtlas Area....

  16. Efficacy of vaccination with recombinant vaccinia and fowlpox vectors expressing NY-ESO-1 antigen in ovarian cancer and melanoma patients.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; Karbach, Julia; Neumann, Antje; Mhawech-Fauceglia, Paulette; Miller, Austin; Beck, Amy; Morrison, Carl D; Ritter, Gerd; Godoy, Heidi; Lele, Shashikant; duPont, Nefertiti; Edwards, Robert; Shrikant, Protul; Old, Lloyd J; Gnjatic, Sacha; Jäger, Elke

    2012-04-10

    Recombinant poxviruses (vaccinia and fowlpox) expressing tumor-associated antigens are currently being evaluated in clinical trials as cancer vaccines to induce tumor-specific immune responses that will improve clinical outcome. To test whether a diversified prime and boost regimen targeting NY-ESO-1 will result in clinical benefit, we conducted two parallel phase II clinical trials of recombinant vaccinia-NY-ESO-1 (rV-NY-ESO-1), followed by booster vaccinations with recombinant fowlpox-NY-ESO-1 (rF-NY-ESO-1) in 25 melanoma and 22 epithelial ovarian cancer (EOC) patients with advanced disease who were at high risk for recurrence/progression. Integrated NY-ESO-1-specific antibody and CD4(+) and CD8(+) T cells were induced in a high proportion of melanoma and EOC patients. In melanoma patients, objective response rate [complete and partial response (CR+PR)] was 14%, mixed response was 5%, and disease stabilization was 52%, amounting to a clinical benefit rate (CBR) of 72% in melanoma patients. The median PFS in the melanoma patients was 9 mo (range, 0-84 mo) and the median OS was 48 mo (range, 3-106 mo). In EOC patients, the median PFS was 21 mo (95% CI, 16-29 mo), and median OS was 48 mo (CI, not estimable). CD8(+) T cells derived from vaccinated patients were shown to lyse NY-ESO-1-expressing tumor targets. These data provide preliminary evidence of clinically meaningful benefit for diversified prime and boost recombinant pox-viral-based vaccines in melanoma and ovarian cancer and support further evaluation of this approach in these patient populations.

  17. Urban metabolism in Syracuse, NY – introduction

    Science.gov (United States)

    David J. Nowak

    2016-01-01

    This special issue of Urban Ecosystems contains a series of papers related to assessing urban metabolism in Syracuse, NY. These papers were developed under the Urban Long-Term Research Area Exploratory Awards Program funded by the National Science Foundation. Objectives of this two-year project (2009–2011) in Syracuse, NY were to investigate: a)...

  18. Urine antibody against human cancer antigen NY-ESO-1

    OpenAIRE

    Jäger, Dirk; Stockert, Elisabeth; Karbach, Julia; Herrlinger, Kristina; Atmaca, Akin; Arand, Michael; Chen, Yao-Tseng; Gnjatic, Sacha; Old, Lloyd J.; Knuth, Alexander; Jäger, Elke

    2002-01-01

    NY-ESO-1 is one of the most immunogenic tumor antigens known to date. Spontaneous humoral and cellular immune responses against NY-ESO-1 are detected in a substantial proportion of patients with NY-ESO-1 positive cancers. NY-ESO-1 serum antibody is dependent on the presence of NY-ESO-1+ cancer cells, and antibody titers correlate with the clinical development of the disease. NY-ESO-1 serum antibody is associated with detectable NY-ESO-1-specific CD8+ T cell reactivity. High titers of NY-ESO-1...

  19. 77 FR 69761 - Regulated Navigation Area; Recovery Operations, the Port of New York and New Jersey, NJ and NY

    Science.gov (United States)

    2012-11-21

    ... waterway users, the public, and pollution response and recovery workers for the duration of the response... vessels could pose significant risk of injury or death to response and recovery workers. Certain portions... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA11 Regulated Navigation Area; Recovery Operations, the...

  20. 2007 USGS/NASA Experimental Advanced Airborne Research Lidar (EAARL): Fire Island National Seashore, NY and Sandy Hook, NJ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — ASCII xyz point cloud data were produced from remotely sensed, geographically referenced elevation measurements cooperatively by the U.S. Geological Survey (USGS)...

  1. Analyses of otoliths from juvenile black sea bass collected from estuaries in SC, FL, NJ, and NY: 2008-2009.

    Data.gov (United States)

    Baruch Institute for Marine and Coastal Sciences, Univ of South Carolina — Snappers/Groupers have traditionally been some of the most desired demersal fishes in the South Atlantic Bight and are the most abundant and diverse group of large...

  2. Cistern Performance for Stormwater Management in Camden, NJ

    Science.gov (United States)

    The Camden County Municipal Utilities Authority (CCMUA) installed cisterns at locations around the city of Camden, NJ. Cisterns provide a cost effective approach to reduce stormwater runoff volume and peak discharge. The collected water can be substituted for potable water in s...

  3. 75 FR 13681 - Television Broadcasting Services; Atlantic City, NJ

    Science.gov (United States)

    2010-03-23

    ... COMMISSION 47 CFR Part 73 Television Broadcasting Services; Atlantic City, NJ AGENCY: Federal Communications... amended, to allocate not less than one very high frequency commercial television channel to each State, if... Review Act, see 5 U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Television,...

  4. 78 FR 7848 - New Jersey Disaster Number NJ-00033

    Science.gov (United States)

    2013-02-04

    ... ADMINISTRATION New Jersey Disaster Number NJ-00033 AGENCY: U.S. Small Business Administration. ACTION: Amendment...: U.S. Small Business Administration, Processing and Disbursement Center, 14925 Kingsport Road, Fort... Business Administration, 409 3rd Street SW., Suite 6050, Washington, DC 20416. SUPPLEMENTARY...

  5. 76 FR 60732 - Drawbridge Operation Regulations; Navesink (Swimming) River, NJ

    Science.gov (United States)

    2011-09-30

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Navesink (Swimming) River, NJ AGENCY... the Oceanic Bridge at mile 4.5 across the Navesink (Swimming) River between Oceanic and Locust Point...-9826. SUPPLEMENTARY INFORMATION: The Oceanic Bridge, across the Navesink (Swimming) River, mile 4.5...

  6. 75 FR 38712 - Drawbridge Operation Regulation; Shrewsbury River, NJ

    Science.gov (United States)

    2010-07-06

    ... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Shrewsbury River, NJ... operation regulations that govern the operation of the Route 36 Bridge at mile 1.8, across the Shrewsbury... operation regulations for the Route 36 Bridge at mile 1.8, across the Shrewsbury River at Highlands,...

  7. Rescuing the nonjet (NJ) azimuth quadrupole from the flow narrative

    CERN Document Server

    Trainor, Thomas A

    2016-01-01

    According to the flow narrative commonly applied to high-energy nuclear collisions a cylindrical-quadrupole component of 1D azimuth angular correlations is conventionally denoted by quantity $v_2$ and interpreted to represent elliptic flow. Jet angular correlations may also contribute to $v_2$ data as "nonflow" depending on the method used to calculate $v_2$, but 2D graphical methods are available to insure accurate separation. The nonjet (NJ) quadrupole has various properties inconsistent with a flow interpretation, including the observation that NJ quadrupole centrality variation in A-A collisions has no relation to strongly-varying jet modification ("jet quenching") in those collisions commonly attributed to jet interaction with a flowing dense medium. In this presentation I describe isolation of quadrupole spectra from pt-differential $v_2(p_t)$ data from the RHIC and LHC. I demonstrate that quadrupole spectra have characteristics very different from the single-particle spectra for most hadrons, that quad...

  8. Complete genome sequence of Aggregatibacter (Haemophilus) aphrophilus NJ8700.

    Science.gov (United States)

    Di Bonaventura, Maria Pia; DeSalle, Rob; Pop, Mihai; Nagarajan, Niranjan; Figurski, David H; Fine, Daniel H; Kaplan, Jeffrey B; Planet, Paul J

    2009-07-01

    We report the finished and annotated genome sequence of Aggregatibacter aphrophilus strain NJ8700, a strain isolated from the oral flora of a healthy individual, and discuss characteristics that may affect its dual roles in human health and disease. This strain has a rough appearance, and its genome contains genes encoding a type VI secretion system and several factors that may participate in host colonization.

  9. Complete Genome Sequence of Aggregatibacter (Haemophilus) aphrophilus NJ8700▿

    Science.gov (United States)

    Di Bonaventura, Maria Pia; DeSalle, Rob; Pop, Mihai; Nagarajan, Niranjan; Figurski, David H.; Fine, Daniel H.; Kaplan, Jeffrey B.; Planet, Paul J.

    2009-01-01

    We report the finished and annotated genome sequence of Aggregatibacter aphrophilus strain NJ8700, a strain isolated from the oral flora of a healthy individual, and discuss characteristics that may affect its dual roles in human health and disease. This strain has a rough appearance, and its genome contains genes encoding a type VI secretion system and several factors that may participate in host colonization. PMID:19447908

  10. Multikulturalisme er en ny slags Apartheid

    DEFF Research Database (Denmark)

    Dyrberg, Torben Bech

    2016-01-01

    I en ny antologi retter forfatterne et kritisk lys mod forsvarerne af multikulturalisme. Og det er der god grund til, mener en af bogens redaktører. For den store gruppe, der hylder multikulturen, glemmer i al dens godhed, at de samtidig er med til at undergrave demokratiet og styrke uheldige mag...

  11. Multikulturalisme er en ny slags Apartheid

    DEFF Research Database (Denmark)

    Dyrberg, Torben Bech

    2016-01-01

    I en ny antologi retter forfatterne et kritisk lys mod forsvarerne af multikulturalisme. Og det er der god grund til, mener en af bogens redaktører. For den store gruppe, der hylder multikulturen, glemmer i al dens godhed, at de samtidig er med til at undergrave demokratiet og styrke uheldige mag...

  12. Mangfoldighed som ny EU-politik

    DEFF Research Database (Denmark)

    Thomsen, Steen

    2014-01-01

    Analyse. EU er stadig i krise. EU er endnu ikke kommet med i det globale opsving efter finanskrisen, og Sydeuropas strukturproblemer er uløste, men maskeret af statsstøtte via ECB. Vælgerne gav EU-politikerne en alvorlig advarsel ved valget til Europa-Parlamentet. Efter en bølge af ny regulering er...

  13. 2008 FEMA Lidar: South Oneida County (NY)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — For Oneida County, NY, there were two types of elevation datasets. The first type is LiDAR and the second one is Auto-correlation DEM. Auto-correlation DEM data was...

  14. Processing and cross-presentation of individual HLA-A, -B, or -C epitopes from NY-ESO-1 or an HLA-A epitope for Melan-A differ according to the mode of antigen delivery.

    Science.gov (United States)

    Robson, Neil C; McAlpine, Tristan; Knights, Ashley J; Schnurr, Max; Shin, Amanda; Chen, Weisan; Maraskovsky, Eugene; Cebon, Jonathan

    2010-07-15

    The ability of dendritic cells (DCs) to cross-present protein tumor antigens to cytotoxic T lymphocytes (CTLs) underpins the success of therapeutic cancer vaccines. We studied cross-presentation of the cancer/testis antigen, NY-ESO-1, and the melanoma differentiation antigen, Melan-A by human DC subsets. Monocyte-derived DCs (MoDCs) efficiently cross-presented human leukocyte associated (HLA)-A2-restricted epitopes from either a formulated NY-ESO-1/ISCOMATRIX vaccine or when either antigen was mixed with ISCOMATRIX adjuvant. HLA-A2 epitope generation required endosomal acidification and was proteasome-independent for NY-ESO-1 and proteasome-dependent for Melan-A. Both MoDCs and CD1c(+) blood DCs cross-presented NY-ESO-1-specific HLA-A2(157-165)-, HLA-B7(60-72)-, and HLA-Cw3(92-100)-restricted epitopes when formulated as an NY-ESO-1/ISCOMATRIX vaccine, but this was limited when NY-ESO-1 and ISCOMATRIX adjuvant were added separately to the DC cultures. Finally, cross-presentation of NY-ESO-1(157-165)/HLA-A2, NY-ESO-1(60-72)/HLA-B7, and NY-ESO-1(92-100)/HLA-Cw3 epitopes was proteasome-dependent when formulated as immune complexes (ICs) but only proteasome-dependent for NY-ESO-1(60-72)/HLA-B7-restricted cross-presentation facilitated by ISCOMATRIX adjuvant. We demonstrate, for the first time, proteasome-dependent and independent cross-presentation of HLA-A-, B-, and C-restricted epitopes within the same full-length tumor antigen by human DCs. Our findings identify important differences in the capacities of human DC subsets to cross-present clinically relevant, full-length tumor antigens and how vaccine formulation impacts CTL responses in vivo.

  15. 77 FR 55455 - Foreign-Trade Zone 235-Lakewood, NJ, Authorization of Production Activity, Cosmetic Essence...

    Science.gov (United States)

    2012-09-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE Foreign-Trade Zones Board Foreign-Trade Zone 235--Lakewood, NJ, Authorization of Production Activity, Cosmetic Essence Innovations, LLC, (Fragrance Bottling), Holmdel, NJ Cosmetic Essence Innovations, LLC...

  16. Mot en ny pedagogisk lederrolle og lederidentitet?

    Directory of Open Access Journals (Sweden)

    Ann Kristin Larsen

    2014-02-01

    Full Text Available Artikkelen omhandler hvordan endrede ledelsesstrukturer oppstått gjennom ny organisasjonsmodell virker inn på de pedagogiske ledernes arbeidsoppgaver. Vi har etterspurt deres erfaringer knyttet til mulige endringer i oppgaver, arbeidsdeling og faglig-pedagogisk fokus i arbeidet. Undersøkelsen ble gjennomført i en bydel i Oslo. Hovedfunnene er at pedagogiske ledere har fått flere administrative oppgaver og personalleder oppgaver. De pedagogiske lederne deler seg i to med hensyn til hvor fornøyde de er med disse endringene. I artikkelen drøfter vi hva denne todelingen betyr for deres rolleforståelse og identitet.

  17. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Science.gov (United States)

    2010-07-01

    ... Light 322° true across the southwest tangent of the island on the north side of Jones Inlet to the... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock Inlet West Breakwater Light 1. (b) A line drawn from Moriches Inlet East Breakwater Light to...

  18. Faget almen dannelse og ny teknologi

    DEFF Research Database (Denmark)

    Tiemensma, Britt Due

    2015-01-01

    16 lektioner anvendes Skoletube, en digital platform rettet mod folkeskolen samt gratis programmer, som jeg forestiller mig vil imødekomme de studerendes behov for sjove oplevelser og samtidig ruste dem til at forholde sig reflekteret og nuanceret til etiske, politiske, demokratiske og religiøse......Denne case handler om, hvordan 90 lærerstuderende på 1. årg. introduceres til faget almen dannelse ud fra en antagelse om, at ny teknologi påvirker forestillingen om, hvad der er relevant viden for professionsudøvelsen, og således også hvad der skal prioriteres i studiet. I introduktionsforløbet på...... udfordringer i relation til skole- og forældresamarbejde. Udfordringen er altså at få ny teknologi til at indgå som aktør i faget almen dannelse og skabe en eksemplarisk praksis, hvor de studerende kan udvikle sig fagligt og personligt i forhold til kommende undervisningsopgaver i folkeskolen....

  19. 33 CFR 110.208 - Buffalo Harbor, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage...

  20. 33 CFR 110.84b - Buffalo, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo, N.Y. 110.84b Section 110.84b Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.84b Buffalo, N.Y. The area within the Port of Buffalo known as...

  1. NY-ESO-1 specific antibody and cellular responses in melanoma patients primed with NY-ESO-1 protein in ISCOMATRIX and boosted with recombinant NY-ESO-1 fowlpox virus.

    Science.gov (United States)

    Chen, Ji-Li; Dawoodji, Amina; Tarlton, Andrea; Gnjatic, Sacha; Tajar, Abdelouahid; Karydis, Ioannis; Browning, Judy; Pratap, Sarah; Verfaille, Christian; Venhaus, Ralph R; Pan, Linda; Altman, Douglas G; Cebon, Jonathan S; Old, Lloyd L; Nathan, Paul; Ottensmeier, Christian; Middleton, Mark; Cerundolo, Vincenzo

    2015-03-15

    Vaccination strategies based on repeated injections of NY-ESO-1 protein formulated in ISCOMATRIX particles (NY-ESO-1 ISCOMATRIX) have shown to elicit combined NY-ESO-1 specific antibody and T cell responses. However, it remains unclear whether heterologous prime-boost strategies based on the combination with NY-ESO-1 ISCOMATRIX with different NY-ESO-1 boosting reagents could be used to increase NY-ESO-1 CD8(+) or CD4(+) T cell responses. To address this question, we carried out a randomized clinical trial in 39 high-risk, resected melanoma patients vaccinated with NY-ESO-1 ISCOMATRIX, and then boosted with repeated injections of either recombinant fowlpox virus encoding full length NY-ESO-1 (rF-NY-ESO-1) (Arm A) or NY-ESO-1 ISCOMATRIX alone (Arm B). We have comprehensively analyzed NY-ESO-1 specific T cells and B cells response in all patients before and after vaccination for a total of seven time points per patient. NY-ESO-1 ISCOMATRIX alone elicited a strong NY-ESO-1 specific CD4(+) T cell and antibody response, which was maintained by both regiments at similar levels. However, CD8(+) T cell responses were significantly boosted in 3 out of 18 patients in Arm A after the first rF-NY-ESO-1 injection and such responses were maintained until the end of the trial, while no patients in Arm B showed similar CD8(+) T cell responses. In addition, our results clearly identified immunodominant regions in the NY-ESO-1 protein: NY-ESO-179-102 and NY-ESO-1115-138 for CD4+ T cells and NY-ESO-185-108 for CD8+ T cells in a large proportion of vaccinated patients. These regions of NY-ESO-1 protein should be considered in future clinical trials as immunodominant epitopes. © 2014 UICC.

  2. 75 FR 54026 - Safety Zone; Red Bull Flugtag, Delaware River, Camden, NJ

    Science.gov (United States)

    2010-09-03

    ... safety zone in an area of the Delaware River, Camden, NJ, described as North of the Wiggins park Marina... traffic from navigating on the Delaware River in an area described as north of the Wiggins Park Marina...

  3. NOAA Digital Oblique Imagery Collection for the Coasts of Main/New Hampshire, Massachusetts/Rhode Island/Connecticut, and Hudson River/Long Island /NY/NJ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Project: NOAA Digital Orthophotography and Ancillary Oblique Imagery Collection for the Coasts of Main/New Hampshire, Massachusetts/Rhode Island/Connecticut, and...

  4. Induction of primary NY-ESO-1 immunity: CD8+ T lymphocyte and antibody responses in peptide-vaccinated patients with NY-ESO-1+ cancers

    OpenAIRE

    Jäger, Elke; Gnjatic, Sacha; Nagata, Yasuhiro; Stockert, Elisabeth; Jäger, Dirk; Karbach, Julia; Neumann, Antje; Rieckenberg, Julia; Chen, Yao-Tseng; Ritter, Gerd; Hoffman, Eric; Arand, Michael; Old, Lloyd J.; Knuth, Alexander

    2000-01-01

    Cancer–testis antigen NY-ESO-1 is one of the most immunogenic tumor antigens defined to date. Spontaneous humoral and CD8+ T-cell responses to NY-ESO-1 are detected in 40–50% of patients with advanced NY-ESO-1-expressing tumors. A clinical trial was initiated to study the immunological effects of intradermal vaccination with 3 HLA-A2-binding NY-ESO-1 peptides in 12 patients with metastatic NY-ESO-1-expressing cancers. Seven patients were NY-ESO-1 serum antibody neg...

  5. Induction of primary NY-ESO-1 immunity: CD8+ T lymphocyte and antibody responses in peptide-vaccinated patients with NY-ESO-1+ cancers

    OpenAIRE

    2000-01-01

    Cancer–testis antigen NY-ESO-1 is one of the most immunogenic tumor antigens defined to date. Spontaneous humoral and CD8+ T-cell responses to NY-ESO-1 are detected in 40–50% of patients with advanced NY-ESO-1-expressing tumors. A clinical trial was initiated to study the immunological effects of intradermal vaccination with 3 HLA-A2-binding NY-ESO-1 peptides in 12 patients with metastatic NY-ESO-1-expressing cancers. Seven patients were NY-ESO-1 serum antibody neg...

  6. EnviroAtlas - New York, NY - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the New York, NY EnviroAtlas community. The block groups are from the US Census Bureau and are included/excluded based...

  7. 77 FR 16800 - Radio Broadcasting Services; Westfield, NY

    Science.gov (United States)

    2012-03-22

    ... From the Federal Register Online via the Government Publishing Office ] FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Westfield, NY AGENCY: Federal Communications..., Radio broadcasting. Federal Communications Commission. Nazifa Sawez, Assistant Chief, Audio...

  8. 枯草芽孢杆菌NJ-18的质粒消解及其在小麦根部的定殖%Plasmid Elimination of Bacillus subtilis NJ-18 and Its Colonization on Wheat Roots

    Institute of Scientific and Technical Information of China (English)

    卢靖乐; 余新燕; 侯毅平; 王建新; 周明国; 陈长军

    2013-01-01

    [目的]研究从土壤中分离出来的具有广谱抗菌活性的枯草芽孢杆菌(Bacillussubtilis)菌株NJ-18与作物互作关系,并观察外源导入绿色荧光蛋白(green fluorescent protein,GFP)基因的NJ-18菌株在小麦根部的定殖能力。[方法]首先使用十二烷基硫酸钠(SDS)消解NJ-18的质粒,然后采用化学法导入gfp分子标记,并采用激光共聚焦显微镜检测该标记菌株在小麦根部的定殖能力。[结果]获得了NJ-18的质粒消解菌株C136,并采用化学方法成功将外源基因导入野生型菌株NJ-18及其质粒消解菌株C136,C136的转化效率可达到3.42×105 cfu/μg质粒DNA,是NJ-18转化效率的10倍左右。对比NJ-18与C136的生物活性发现,C136能够保持原有的抑菌活性;但是C136的生长曲线第一峰值出现的时间滞后12 h,第一峰值被抑制了29.4%;NJ-18-GFP能够附着在病原菌菌体上,导致其菌丝呈现膨大、畸形等;接种7d后能够在小麦根部表皮和中柱稳定定殖。[结论]枯草芽孢杆菌NJ-18菌株的转化方法宜选用化学法;该菌株能够在小麦根部定殖。%[Objective]Bacillus subtilis strain NJ-18, isolated from the soil of oilseed rape field, has a broad spectrum antibacterial activity and a great potential for biocontrol. In order to study the interaction between NJ-18 and plants, NJ-18 was marked with a green fluorescent protein (gfp) gene and its colonization ability on wheat roots was observed with a laser scanning confocal microscope.[Method]The plasmids of NJ-18 were cured by sodium dodecyl sulfate (SDS) and gfp was transformed into the strain with a chemical method. Colonization ability of NJ-18 on wheat roots was observed with a laser scanning confocal microscope. [Result] By a chemical method, gfp was sucessfully transformed into wild strain NJ-18 and C136, a plasmid cured strain from NJ-18. The transformation efficiency of C136 could be up to 3.42×105 cfu/μg of

  9. Middle Holocene Unconformity in Seneca Lake, NY

    Science.gov (United States)

    Curtin, T. M.; Crocker, M.; Loddengaard, K.

    2008-12-01

    The post-glacial history of the Finger Lakes, NY have involved several changes in lake levels throughout the latest Pleistocene and Holocene, resulting from the changing position of the retreating Laurentide ice sheet, river outlet position, glacial rebound, and water balance. Previous studies of high-resolution seismic reflection profiles from three Finger Lakes define a middle Holocene erosional surface at water depths as great as 26 m in the northern end of each of lake. There are two proposed hypotheses to explain the origin of the observed erosional surfaces: 1) subaerial erosion during a lake lowstand and 2) erosion resulting from increased internal seiche activity. To evaluate these hypotheses, we examined a series of 2 to 5 m long piston cores collected along a north-south transect from one of the Finger Lakes, Seneca Lake. Cores were correlated using distinctive changes in the profiles of grain size, loss-on-ignition, and magnetic susceptibility. We recognize a significant erosional unconformity of early to middle Holocene sediment at modern depths 60 m, the unconformity continues as a conformable zone. We attribute the unconformity to wave abrasion and nearshore current winnowing of the shoreface during a lowstand. With an assumption of an effective 20 m wave base, the depth to the low level lake surface responsible for the unconformity is estimated to be 40 m. The age of the unconformity is ~6 ka, based on radiocarbon ages of lithologic boundaries in the sediment cores. Because the unconformity grades into a conformable zone in deepwater cores that display no change in lithology, we hypothesize that the large-scale lake level drop is likely not the result of climate change, but rather a change in accommodation space in the northern portion of the lake basin due to glacial rebound.

  10. Autoimmune thyroid disease elicited by NY-ESO-1 vaccination.

    Science.gov (United States)

    Vita, Roberto; Guarneri, Fabrizio; Agah, Ravin; Benvenga, Salvatore

    2014-02-01

    Immunotherapies and targeted therapies are frequently associated with thyroid dysfunction, which is in contrast with the rare thyroid abnormalities induced by cytotoxic agents. Immunotherapy with NY-ESO-1, a tumor-associated antigen expressed by a number of malignancies, was reported to trigger hyperthyroidism or hypothyroidism in two HLA-A2 patients with ovarian cancer. We describe now a case of Graves' disease triggered by NY-ESO-1 in a HLA-A2-negative woman. A 32-year-old woman with a synovial sarcoma received radiotherapy, chemotherapy, and finally NY-ESO-1 vaccine. The patient was found to have HLA A11/A33(19), B13/B56(22), Cw3/-. One month after the beginning of immunotherapy, thyroid dysfunction was clinically suspected and Graves' disease was biochemically confirmed. Fearful of the antithyroid drugs' side effects, the patient was treated with a beta-blocker (propranolol, 80-20 mg/day). As hyperthyroidism progressively worsened, the patient underwent total thyroidectomy. We hypothesized that NY-ESO-1 shared partial homology with thyroid autoantigens (the so-called molecular mimicry mechanism) and that at least one pair of homologous sequences contained amino acid sequence binding motifs to a restricted number of HLA molecules. We used BLAST software to search amino acid sequence homologies between NY-ESO-1 and thyroid autoantigens (thyrotropin receptor [TSH-R], thyroperoxidase, and thyroglobulin), and the HLA ligand/motif database to look for HLA/T-cell receptor binding motifs in the regions of NY-ESO-1 and thyroid autoantigens that were homologous. We found 15 epitopic regions of NY-ESO-1 homologous to 15 regions of thyroid autoantigens, some of which epitopic: 5 of TSH-R, 8 of thyroglobulin, and 2 of thyroperoxidase. These homologous sequences contain binding motifs belonging to several HLA class I antigens, including HLA A2 and the patient's A11 and A33. Genetically predisposed patients who receive NY-ESO-1 vaccination are at risk to develop thyroid

  11. En ny generasjon troverdige kliniske retningslinjer

    Directory of Open Access Journals (Sweden)

    Per Olav Vandvik

    2013-11-01

    Full Text Available Trygg og effektiv diagnostikk, pleie og behandling fordrer at helsepersonell har tilgang til oppdatert og gyldig forskningsbasert kunnskap, fortrinnsvis i form av faglige kliniske retningslinjer utviklet med tilfredsstillende metodikk. De fleste retningslinjer har imidlertid betydelige svakheter med hensyn til både kunnskapsgrunnlag, utvikling av anbefalinger, presentasjonsformater og oppdatering. Nye definisjoner, standarder og metoder for kunnskapsbaserte troverdige retningslinjer gir langt bedre muligheter enn tidligere for å lykkes i arbeidet med å utvikle og implementere retningslinjer, men stiller samtidig større krav til metodekompetanse, klinisk ekspertise og tid. I vår digitale tidsalder åpner ny nettbasert teknologi for bedre disseminering av retningslinjer til frontlinjene i klinikken, og til å dele kunnskap med pasienter i konsultasjonen, for eksempel gjennom smarttelefoner og lesebrett. Gjennom et praktisk eksempel redegjør vi i denne artikkelen for nye definisjoner, standarder, metoder og verktøy for utvikling, disseminering og oppdatering av faglige retningslinjer. GRADE-systemet er en sentral komponent i dette. Det er nå mulig å lage troverdige retningslinjer som klinikere og pasienter kan ta i bruk i en travel hverdag. Det gjenstår å se hvordan myndighetene og fagmiljøene vil bidra for å sikre pasienter trygg og effektiv behandling gjennom utvikling av denne nye generasjonen av kliniske retningslinjer.Vandvik PO, Berg R, Vist G. A new generation of trustworthy clinical practice guidelines. Nor J Epidemiol 2013; 23 (2: 197-204.ENGLISH SUMMARYSafe and effective diagnosis and treatment requires that health care personnel can access the best current research evidence, preferably through clinical practice guidelines. Most guidelines suffer from methodological weaknesses (e.g., identification and assessment of research evidence, development of recommendations, suboptimal presentation formats and infrequent updating of

  12. 33 CFR 334.100 - Atlantic Ocean off Cape May, N.J.; Coast Guard Rifle Range.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Atlantic Ocean off Cape May, N.J.; Coast Guard Rifle Range. 334.100 Section 334.100 Navigation and Navigable Waters CORPS OF ENGINEERS... Ocean off Cape May, N.J.; Coast Guard Rifle Range. (a) The danger zone. The waters of the Atlantic...

  13. 76 FR 11679 - Drawbridge Operation Regulation; Shark River (South Channel), Belmar, NJ

    Science.gov (United States)

    2011-03-03

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulation; Shark River (South Channel), Belmar... operation of the S71 Bridge across Shark River (South Channel), mile 0.8, at Belmar, NJ. The deviation is... INFORMATION: The S71 Bridge, a bascule lift drawbridge, across Shark River (South Channel), at mile 0.8,...

  14. 77 FR 70372 - Drawbridge Operation Regulation; Shark River (South Channel), Avon Township, NJ

    Science.gov (United States)

    2012-11-26

    ... SECURITY Coast Guard 33 CFR Part 117 RIN 1625-AA09 Drawbridge Operation Regulation; Shark River (South..., across Shark River (South Channel) at Avon Township, NJ. The existing regulation contains a drawbridge... Transportation (NJDOT) to replace the existing bascule bridge, which carries S35 over Shark River (South...

  15. 33 CFR 162.30 - Channel of Tuckerton Creek, N.J.; navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Channel of Tuckerton Creek, N.J.; navigation. 162.30 Section 162.30 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS § 162.30 Channel...

  16. 77 FR 14690 - Drawbridge Operation Regulation; New Jersey Intracoastal Waterway (NJICW), Point Pleasant Canal, NJ

    Science.gov (United States)

    2012-03-13

    ...The Commander, Fifth Coast Guard District, has issued a temporary deviation from the regulations governing the operation of the Route 88/Veterans Memorial Bridge across Point Pleasant Canal, NJICW mile 3.0, at Point Pleasant, NJ. This deviation allows the bridge to remain closed-to-navigation in order to facilitate barrier gate replacement and extensive electrical remedial work on the...

  17. 78 FR 21849 - Television Broadcasting Services; Ely, NV to Middletown Township, NJ

    Science.gov (United States)

    2013-04-12

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; Ely, NV to Middletown Township, NJ AGENCY... U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Television. Federal...

  18. 77 FR 27115 - Drawbridge Operation Regulations; Raritan River, Perth Amboy, NJ

    Science.gov (United States)

    2012-05-09

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Raritan River, Perth Amboy, NJ... Perth Amboy, New Jersey. Under this temporary deviation the draw may remain in the closed position for..., mile 0.5, at Perth Amboy, New Jersey, has a vertical clearance in the closed position of 8 feet at...

  19. The Oil Game: Generating Enthusiasm for Geosciences in Urban Youth in Newark, NJ

    Science.gov (United States)

    Gates, Alexander E.; Kalczynski, Michael J.

    2016-01-01

    A hands-on game based upon principles of oil accumulation and drilling was highly effective at generating enthusiasm toward the geosciences in urban youth from underrepresented minority groups in Newark, NJ. Participating 9th-grade high school students showed little interest in the geosciences prior to participating in the oil game, even if they…

  20. 77 FR 46613 - Safety Zone; 2012 Ironman US Championship Swim, Hudson River, Fort Lee, NJ

    Science.gov (United States)

    2012-08-06

    ...) entitled 2012 Ironman US Championship Swim, Hudson River, Fort Lee, NJ in the Federal Register (77 FR 34285...) 366-9826. SUPPLEMENTARY INFORMATION: Table of Acronyms DHS Department of Homeland Security FR Federal... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; 2012 Ironman US Championship Swim,...

  1. Managing a Statewide Virtual Reference Service: How Q and A NJ Works.

    Science.gov (United States)

    Bromberg, Peter

    2003-01-01

    Describes the live virtual reference service, Q and A NJ (Question and Answer New Jersey), strategies used to meet the challenges of day-to-day management, scaled growth and quality control. Describes how it began; how long it took; how to manage a large project (constant communication; training and practice; transcript analysis and privacy;…

  2. 78 FR 45957 - Notice of Inventory Completion: Columbia University, Department of Anthropology, New York, NY

    Science.gov (United States)

    2013-07-30

    ..., Columbia University, New York, NY 10027, telephone (212) 854-4977, email roth@columbia.edu . SUPPLEMENTARY... York, NY 10027, telephone (212) 854-4977, email roth@columbia.edu , by August 29, 2013. After that...

  3. 智慧校园背景下的高校治安防控体系建设研究--以 NJ 高校为例%Study on the Construction of Public Security Prevention and Control System in Higher Colleges under the Background of Wisdom Campus -Take NJ Higher College as the Example

    Institute of Scientific and Technical Information of China (English)

    谢秉宸

    2016-01-01

    本文从高校治安防控工作存在的问题入手,分析 NJ 高校当前治安管理工作存在的难点及其原因,基于智慧校园背景,从构建校园应急指挥系统、引入智能停车场管理系统和袖珍公共交通系统以及建立健全群防群治网络等四个方面,提出建立高校治安防控体系建设的构想。%From the crime prevention and control work in higher colleges,there exists difficulties and reason in the work of NY of the current security management.Based on the background of the wisdom of the campus,we can put forward conception of the establishment of the crime prevention and control system from four aspects of the construction of campus emergency com-mand system,the introduction to the intelligent parking management system,the pocket public transport system as well as es-tablishing and improving the prevention and treatment network.

  4. Evaluering af ny kokkeuddannelse for hørehandicappede

    DEFF Research Database (Denmark)

    Hansen, Niels-Henrik Møller

    2010-01-01

    Center for Ungdomsforskning har netop afsluttet en evaluering af en ny uddannelse indenfor madproduktion, rettet imod personer med et hørehandicap. Hensigten med uddannelsen er, at kunne tilbyde døve, døvblinde og hørehæmmede personer et uddannelsestilbud indenfor fremstilling af mad. Konkret han...

  5. 78 FR 44187 - New York Disaster # NY-00136

    Science.gov (United States)

    2013-07-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster NY-00136 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... locations. The following areas have been determined to be adversely affected by the disaster:...

  6. Evaluering af ny kokkeuddannelse for hørehandicappede

    DEFF Research Database (Denmark)

    Hansen, Niels-Henrik Møller

    2010-01-01

    Center for Ungdomsforskning har netop afsluttet en evaluering af en ny uddannelse indenfor madproduktion, rettet imod personer med et hørehandicap. Hensigten med uddannelsen er, at kunne tilbyde døve, døvblinde og hørehæmmede personer et uddannelsestilbud indenfor fremstilling af mad. Konkret han...

  7. Kapitalismens dna i ny oversættelse

    DEFF Research Database (Denmark)

    Schanz, Hans-Jørgen

    2013-01-01

    Adam Smiths klassiske storværk om kapitalismen ’Nationernes velstand’ havde stor indflydelse på socialfilosofien, på Hegel, på Marx og liberaløkonomerne. Ny oversættelse yder omsider værket fuld respekt på dansk. Anmeldelse af: Adam Smith: Nationernes velstand. 441+ 519 sider. 699,95 kr. Informat...

  8. 78 FR 7848 - New York Disaster Number NY-00130

    Science.gov (United States)

    2013-02-04

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5.... Small Business Administration, Processing and Disbursement Center, 14925 Kingsport Road, Fort Worth,...

  9. Ny international efteruddannelse for fødevaresektoren

    DEFF Research Database (Denmark)

    Stacey, Julia; Brunsø, Karen

    2003-01-01

    Ny MBA på Handelshøjskolen i Århus stiller skarpt på fødevaresektoren. MBA - med fokus på fødevaresektoren henvender sig til personer med minimum tre års ledererfaring, der ønsker nye erhvervsrettede kompetencer på højt niveau....

  10. Kapitalismens dna i ny oversættelse

    DEFF Research Database (Denmark)

    Schanz, Hans-Jørgen

    2013-01-01

    Adam Smiths klassiske storværk om kapitalismen ’Nationernes velstand’ havde stor indflydelse på socialfilosofien, på Hegel, på Marx og liberaløkonomerne. Ny oversættelse yder omsider værket fuld respekt på dansk. Anmeldelse af: Adam Smith: Nationernes velstand. 441+ 519 sider. 699,95 kr. Informat...

  11. Interoperability of Demand Response Resources Demonstration in NY

    Energy Technology Data Exchange (ETDEWEB)

    Wellington, Andre

    2014-03-31

    The Interoperability of Demand Response Resources Demonstration in NY (Interoperability Project) was awarded to Con Edison in 2009. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited Demand Response resources to integrate more effectively with electric delivery companies and regional transmission organizations.

  12. 77 FR 50630 - Radio Broadcasting Services; Westfield, NY

    Science.gov (United States)

    2012-08-22

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Westfield, NY AGENCY: Federal Communications... Review Act, see 5 U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Radio, Radio...

  13. NY-ESO-1 cancer testis antigen demonstrates high immunogenicity in triple negative breast cancer.

    Science.gov (United States)

    Ademuyiwa, Foluso O; Bshara, Wiam; Attwood, Kristopher; Morrison, Carl; Edge, Stephen B; Karpf, Adam R; James, Smith A; Ambrosone, Christine B; O'Connor, Tracey L; Levine, Ellis G; Miliotto, Anthony; Ritter, Erika; Ritter, Gerd; Gnjatic, Sacha; Odunsi, Kunle

    2012-01-01

    NY-ESO-1 cancer testis (CT) antigen is an attractive candidate for immunotherapy as a result of its high immunogenicity. The aim of this study was to explore the potential for NY-ESO-1 antigen directed immunotherapy in triple negative breast cancer (TNBC) by determining the frequency of expression by immunohistochemistry (IHC) and the degree of inherent immunogenicity to NY-ESO-1. 168 TNBC and 47 ER+/HER2- primary breast cancer specimens were used to determine NY-ESO-1 frequency by IHC. As previous studies have shown that patients with a robust innate humoral immune response to CT antigens are more likely to develop CD8 T-cell responses to NY-ESO-1 peptides, we evaluated the degree to which patients with NY-ESO-1 expression had inherent immunogenicity by measuring antibodies. The relationship between NY-ESO-1 expression and CD8+ T lymphocytes was also examined. The frequency of NY-ESO-1 expression in the TNBC cohort was 16% versus 2% in ER+/HER2- patients. A higher NY-ESO-1 score was associated with a younger age at diagnosis in the TNBC patients with NY-ESO-1 expression (p = 0.026). No differences in OS (p = 0.278) or PFS (p = 0.238) by NY-ESO-1 expression status were detected. Antibody responses to NY-ESO-1 were found in 73% of TNBC patients whose tumors were NY-ESO-1 positive. NY-ESO-1 positive patients had higher CD8 counts than negative patients (p = 0.018). NY-ESO-1 is expressed in a substantial subset of TNBC patients and leads to a high humoral immune response in a large proportion of these individuals. Given these observations, patients with TNBC may benefit from targeted therapies directed against NY-ESO-1.

  14. Properties of Superconducting Mo, Mo2n and Trilayer Mo2n-Mo-Mo2n Thin Films

    Science.gov (United States)

    Barrentine, E. M.; Stevenson, T. R.; Brown, A. D.; Lowitz, A. E.; Noroozian, O.; U-Yen, K.; Eshan, N.; Hsieh, W. T.; Moseley, S. H.; Wollack, E. J.

    2014-01-01

    We present measurements of the properties of thin film superconducting Mo, Mo2N and Mo2N/Mo/Mo2N trilayers of interest for microwave kinetic inductance detector (MKID) applications. Using microwave resonator devices, we investigate the transition temperature, energy gaps, kinetic inductance, and internal quality factors of these materials. We present an Usadel-based interpretation of the trilayer transition temperature as a function of trilayer thicknesses, and a 2-gap interpretation to understand the change in kinetic inductance and internal resonance quality factor (Q) as a function of temperature.

  15. 33 CFR 207.60 - Federal Dam, Hudson River, Troy, N.Y.; pool level.

    Science.gov (United States)

    2010-07-01

    ..., N.Y.; pool level. 207.60 Section 207.60 Navigation and Navigable Waters CORPS OF ENGINEERS..., N.Y.; pool level. (a) Whenever the elevation of the pool created by the Federal dam at Troy, N.Y... automatically when the pool level rises to an elevation of +18.5 feet mean sea level, and conform in...

  16. 76 FR 20532 - Safety Zone; Boom Days, Niagara River, Niagara Falls, NY

    Science.gov (United States)

    2011-04-13

    ..., Niagara River, Niagara Falls, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the Niagara River, Niagara Falls, NY for the Boom... Niagara River, Niagara Falls, NY during the Boom Days Fireworks on April 16, 2011. This temporary...

  17. Asymptotics of Wigner 3nj-symbols with Small and Large Angular Momenta: an Elementary Method

    CERN Document Server

    Bonzom, Valentin

    2011-01-01

    Yu and Littlejohn recently studied in arXiv:1104.1499 some asymptotics of Wigner symbols with some small and large angular momenta. They found that in this regime the essential information is captured by the geometry of a tetrahedron, and gave new formulae for 9j, 12j and 15j-symbols. We present here an alternative derivation which leads to a simpler formula, based on the use of the Ponzano-Regge formula for the relevant tetrahedron. The approach is generalized to Wigner 3nj-symbols with some large and small angular momenta, where more than one tetrahedron is needed, leading to new asymptotics for Wigner 3nj-symbols. As an illustration, we present 15j-symbols with one, two and four small angular momenta, and give an alternative formula to Yu's recent 15j-symbol with three small spins.

  18. Geothermal energy and district heating in Ny-Ålesund, Svalbard

    OpenAIRE

    Iversen, Julianne

    2013-01-01

    This thesis presents the possibilities for using shallow geothermal energy for heating purposes in Ny-Ålesund. The current energy supply in Ny-Ålesund is a diesel generator, which does not comply with the Norwegian government and Ny-Ålesund Science Managers Committee’s common goal to maintain the natural environment in Ny-Ålesund. Ny-Ålesund has a potential for replacing the heat from the current diesel based energy source with geothermal energy. Geothermal energy is considered to have low im...

  19. Passively Q-switched nanosecond erbium-doped fiber laser with MoS(2) saturable absorber.

    Science.gov (United States)

    Ren, Jun; Wang, Shuxian; Cheng, Zhaochen; Yu, Haohai; Zhang, Huaijin; Chen, Yanxue; Mei, Liangmo; Wang, Pu

    2015-03-09

    Passively Q-switched nanosecond pulsed erbium-doped fiber laser based on MoS(2) saturable absorber (SA) is experimentally demonstrated. The high quality MoS(2) SA deposited on the broadband high-reflectivity mirror with a large modulation depth of 9% was prepared by pulsed laser deposition method. By inserting the MoS(2) SA into an erbium-doped fiber laser, stable Q-switched operation can be achieved with the shortest pulse width of 660 ns, the maximum pulse energy up to 152 nJ and pulse repetition rates varying from 116 to 131 kHz. The experimental results further verify that MoS(2) possesses the potential advantage for stable Q-switched pulse generation at 1.5 μm.

  20. High performance Mo adsorbent PZC

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    1998-10-01

    We have developed Mo adsorbents for natural Mo(n, {gamma}){sup 99}Mo-{sup 99m}Tc generator. Among them, we called the highest performance adsorbent PZC that could adsorb about 250 mg-Mo/g. In this report, we will show the structure, adsorption mechanism of Mo, and the other useful properties of PZC when you carry out the examination of Mo adsorption and elution of {sup 99m}Tc. (author)

  1. Ny forskning: Derfor virker ros ikke mod stress

    DEFF Research Database (Denmark)

    Pedersen, Pernille

    2015-01-01

    Ny forskning peger på, at stressede medarbejdere overhører din ros og anerkendelse, hvis de føler skam. Skam over ikke at kunne slå til på arbejdet. Vil du hjælpe en stresset medarbejder, skal du forebygge, at de føler sig skamfulde. Læs her anbefalinger til, hvordan du bedst hjælper din stressed...

  2. Characterization of the near-Earth Asteroid 2002NY40

    OpenAIRE

    Roberts, Jr., Lewis C.; Hall, Doyle T.; Lambert, John V.; Africano, John L.; Knox, Keith T.; Barros, Jacob K.; Hamada, Kris M.; Liang, Dennis; Sydney, Paul F.; Kervin, Paul

    2007-01-01

    In August 2002, the near-Earth asteroid 2002 NY40, made its closest approach to the Earth. This provided an opportunity to study a near-Earth asteroid with a variety of instruments. Several of the telescopes at the Maui Space Surveillance System were trained at the asteroid and collected adaptive optics images, photometry and spectroscopy. Analysis of the imagery reveals the asteroid is triangular shaped with significant self-shadowing. The photometry reveals a 20-hour period and the spectros...

  3. Den fjerde vej: Trumps sejr skaber ny samfundsorden

    DEFF Research Database (Denmark)

    Rasmussen, Martin Møller Boje; Andersen, Torben K.; Kragh, Claus

    2016-01-01

    Bag Donald Trumps uempatiske retorik gemmer sig omridset af et politisk projekt, man kan kalde den fjerde vej. USA’s kommende præsident blander højre- og venstreorienterede mærkesager til et nyt nationalsocialt politisk koncept. Spørgsmålet er, om Trumps fjerde vej er et seriøst bud på en ny...

  4. MoS₂ decoration by Mo-atoms and the MoS₂-Mo-graphene heterostructure: a theoretical study.

    Science.gov (United States)

    Kvashnin, D G; Sorokin, P B; Seifert, G; Chernozatonskii, L A

    2015-11-21

    Here we propose a completely new covalent heterostructure based on graphene and self-decorated MoS2 monolayers. Detailed investigation of the decoration process of the MoS2 surface by Mo adatoms was performed using first principles DFT methods. Comparison between valence-only and semicore pseudopotentials was performed to correctly describe the interaction between Mo adatoms and the MoS2 surface. It was found that self-decoration by Mo atoms is favorable from an energetic point of view. We studied in detail various decoration paths of Mo atoms on the MoS2 surface. The strong variation of electronic properties after the decoration of MoS2 was found. The impact of the presence of Mo adatoms on the electronic properties of the graphene/MoS2 heterostructure was shown.

  5. NY-ESO-1 antibody as a novel tumour marker of gastric cancer.

    Science.gov (United States)

    Fujiwara, S; Wada, H; Kawada, J; Kawabata, R; Takahashi, T; Fujita, J; Hirao, T; Shibata, K; Makari, Y; Iijima, S; Nishikawa, H; Jungbluth, A A; Nakamura, Y; Kurokawa, Y; Yamasaki, M; Miyata, H; Nakajima, K; Takiguchi, S; Nakayama, E; Mori, M; Doki, Y

    2013-03-19

    NY-ESO-1 antibodies are specifically observed in patients with NY-ESO-1-expressing tumours. We analysed whether the NY-ESO-1 humoral immune response is a useful tumour marker of gastric cancer. Sera from 363 gastric cancer patients were screened by enzyme-linked immunosorbent assay (ELISA) to detect NY-ESO-1 antibodies. Serial serum samples were obtained from 25 NY-ESO-1 antibody-positive patients, including 16 patients with curative resection and 9 patients who received chemotherapy alone. NY-ESO-1 antibodies were detected in 3.4% of stage I, 4.4% of stage II, 25.3% of stage III, and 20.0% of stage IV patients. The frequency of antibody positivity increased with disease progression. When the NY-ESO-1 antibody was used in combination with carcinoembryonic antigen and CA19-9 to detect gastric cancer, information gains of 11.2% in stages III and IV, and 5.8% in all patients were observed. The NY-ESO-1 immune response levels of the patients without recurrence fell below the cutoff level after surgery. Two of the patients with recurrence displayed incomplete decreases. The nine patients who received chemotherapy alone continued to display NY-ESO-1 immune responses. When combined with conventional tumour markers, the NY-ESO-1 humoral immune response could be a useful tumour marker for detecting advanced gastric cancer and inferring the post-treatment tumour load in seropositive patients.

  6. NY-ESO-1 expression and immunogenicity in prostate cancer patients.

    Science.gov (United States)

    Gati, Asma; Lajmi, Nesrine; Derouiche, Amine; Marrakchi, Raja; Chebil, Mohamed; Benammar-Elgaaied, Amel

    2011-10-01

    Prostate cancer is the second leading cause of men cancer-related death. Cancer immunotherapy has been investigated as a treatment which might be instituted at the point of detection of androgen-independent metastatic disease. to investigate the expression and humoral response against NYESO-1 in patients with prostate cancer (PC) and to analyze the relationship between expression of NY-ESO-1 and clinicopathological features. NY-ESO-1 mRNA in surgically resected PC and benign prostatic hyperplasia (BPH) were examined by reverse transcriptionpolymerase chain reaction. The antibody response to NY-ESO-1 was examined by enzyme-linked Elisa assay using recombinant NYESO-1 protein. NY-ESO-1 mRNA was detected in 9 of 23 (39%) PC patients. Antibodies against NY-ESO-1 protein were detected in 12 of 23 (52%) sera of PC patients and in 5 of 9 (55%) of NY-ESO-1 expressing tumors. However, no mRNA copy or NY-ESO-1 antibodies were detected in all BPH patients tested. The present study has demonstrated the expression of NY-ESO-1mRNA in prostate Cancer patients and NY-ESO-1 antibody production. Our data suggest that NY-ESO-1 could be used as a tumor marker and constitute a good candidate for vaccine-based immunotherapy for hormonal resistant prostate cancer patients.

  7. Stability Limits of the Liquid Phase in the Layered Mo/Pb/Mo, Mo/Bi/Mo and Mo/In/Mo Film Systems

    Directory of Open Access Journals (Sweden)

    S.I. Petrushenko

    2016-12-01

    Full Text Available The results of the study of supercooling of the fusible metals films between the continuous layers of molybdenum during the crystallization are given. Due to an original in situ technique based on the changes of resistance of samples during heating and cooling directly in the vacuum chamber the values of supercooling in Mo/Pb/Mo, Mo/Bi/Mo, Mo/In/Mo films are determined and the influence of the morphological structure of the bismuth inclusions on the temperature and nature of its supercooled melt crystallization has been found. The substrate temperature change during the condensation of samples, accompanied by a corresponding change in their microstructure, allows realizing both avalanche and diffuse crystallization and changing of supercooling value in the range of 60 to 180 K in Mo/Bi/Mo films.

  8. Vaccination with NY-ESO-1 overlapping peptides mixed with Picibanil OK-432 and montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Wada, Hisashi; Isobe, Midori; Kakimi, Kazuhiro; Mizote, Yu; Eikawa, Shingo; Sato, Eiichi; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Yamasaki, Makoto; Miyata, Hiroshi; Matsushita, Hirokazu; Udono, Heiichiro; Seto, Yasuyuki; Yamada, Kazuhiro; Nishikawa, Hiroyoshi; Pan, Linda; Venhaus, Ralph; Oka, Mikio; Doki, Yuichiro; Nakayama, Eiichi

    2014-01-01

    We conducted a clinical trial of an NY-ESO-1 cancer vaccine using 4 synthetic overlapping long peptides (OLP; peptides #1, 79-108; #2, 100-129; #3, 121-150; and #4, 142-173) that include a highly immunogenic region of the NY-ESO-1 molecule. Nine patients were immunized with 0.25 mg each of three 30-mer and a 32-mer long NY-ESO-1 OLP mixed with 0.2 KE Picibanil OK-432 and 1.25 mL Montanide ISA-51. The primary endpoints of this study were safety and NY-ESO-1 immune responses. Five to 18 injections of the NY-ESO-1 OLP vaccine were well tolerated. Vaccine-related adverse events observed were fever and injection site reaction (grade 1 and 2). Two patients showed stable disease after vaccination. An NY-ESO-1-specific humoral immune response was observed in all patients and an antibody against peptide #3 (121-150) was detected firstly and strongly after vaccination. NY-ESO-1 CD4 and CD8 T-cell responses were elicited in these patients and their epitopes were identified. Using a multifunctional cytokine assay, the number of single or double cytokine-producing cells was increased in NY-ESO-1-specific CD4 and CD8 T cells after vaccination. Multiple cytokine-producing cells were observed in PD-1 (-) and PD-1 (+) CD4 T cells. In conclusion, our study indicated that the NY-ESO-1 OLP vaccine mixed with Picibanil OK-432 and Montanide ISA-51 was well tolerated and elicited NY-ESO-1-specific humoral and CD4 and CD8 T-cell responses in immunized patients.

  9. Normal dispersion erbium-doped fiber laser with pulse energies above 10 nJ.

    Science.gov (United States)

    Ruehl, Axel; Kuhn, Vincent; Wandt, Dieter; Kracht, Dietmar

    2008-03-03

    We report on an erbium-doped fiber oscillator mode-locked by nonlinear polarization evolution operating in the large normal dispersion regime. The setup produced highly chirped 10 nJ pulses at 37 MHz which can be compressed externally to below 75 fs. Hence, this simple and practical setup is capable of providing ultrashort pulses with a peak power of 140 kW. The pulse formation is indeed subject to intrapulse Raman-scattering but a clean and stable pulse train can be observed. The similarities as well as the differences of the output characteristics to the parabolic pulse and wave breaking-free regime are explicated.

  10. MoEDAL expands

    CERN Multimedia

    Anaïs Schaeffer

    2011-01-01

    The MoEDAL collaboration deployed a test array of 18 plastic Nuclear Track Etch Detector (NTD) stacks – covering an area of 1 m2 – in the MoEDAL/VELO cavern at Point 8 of the LHC ring in November 2009. This small array was supplemented by a further 110 stacks this past January. The MoEDAL test array, which now covers an area of 8 m2, will reveal its secrets early in 2013. The full MoEDAL detector will be installed in the next long shutdown of the LHC in 2013.   View of the MoEDAL detectors installed at Point 8 of the LHC ring in January 2011. MoEDAL (Monopole and Exotics Detector At the LHC), the seventh LHC experiment, was approved by the CERN Research Board at the end of 2009. Its goal is to search for very specific exotics such as highly ionising massive stable (or pseudo-stable) particles with conventional electrical charge and magnetic monopoles. “The main LHC experiments are designed to detect conventionally charged particles, with conventional ionisation patte...

  11. Evaluation of the performance of PRECIL LBY-NJ4A automatic platelet aggregation analyzer%普利生LBY-NJ4A全自动血小板聚集仪性能评价

    Institute of Scientific and Technical Information of China (English)

    石冬敏; 吴元健; 马伟

    2012-01-01

    目的 对普利生LBY-NJ4A全自动血小板聚集仪(NJ4A)进行性能评估.方法 109 mmol/L枸橼酸钠真空管采血,分离富含血小板血浆(PRP)和贫血小板血浆(PPP),应用NJ4A及配套质控品、诱导剂和清洗液,测定血小板最大聚集率(MAR),测试精密度、通道一致性、不确定度、检测限、干扰等.结果 批内不精密度测试,变异系数(CV)为3.4%~5.0%;4个不同通道测定均值差异无统计学意义(P>0.05);携带污染率2.82%;CV批内≤3.5%,CV批间≤4.2%,CV总≤3.8%;总误差范围为5.6%~10.3%,不确定度在可接受范围;2个水平质控品测定值与靶值差异无统计学意义(P>0.05);NJ4A与LBY-NJ2比对结果相关良好(r=0.998,P0.05).结论 NJ4A精密度、准确度、不确定度、灵敏度、携带污染率、抗干扰等性能指标符合CLSI规范,可在临床应用.%Objective To check and evaluate the performance of PRECIL LBY-NJ4A automatic platelet aggregation analyzer (NJ4A) .Methods Blood samples were collected with 109 mmol/L sodium citrate using vacuum tube,then the platelet-rich plasma (PRP)and platelet-poor plasma(PPP) were separated .The NJ4A analyzer with mating quality control,agonists and cleaning solution were used to measure the maximum aggregate rate of platelet (MAR),precision,channel consistency,inaccuracy,detection limitation and inference .Results The precision ranged from 3 .4/0 to 5 .0% .The means of four different channels have no statistically difference(P>0 .05),when carryover rate was 2 .82% .The intra,inter hatch and total coefficient of variance was less than or equal to 3 .5%,4 .2% and 3 .8/0,respectively .The total error ranged from 5 .6/0 to 10 .3%,with an acceptable inaccuracy .Two levels of quality control have no statistically difference with each target value (P>0.05).The correlation of NJ4 and LBY-NJ2 was 0.998 (P0.05) compared to the well-recognized reference range .Conclusion The performance indicators of precision, accuracy, uncertainty

  12. Optical fiber link for transmission of 1-nJ femtosecond laser pulses at 1550 nm.

    Science.gov (United States)

    Eichhorn, Finn; Olsson, Rasmus Kjelsmark; Buron, Jonas C D; Grüner-Nielsen, Lars; Pedersen, Jens Engholm; Jepsen, Peter Uhd

    2010-03-29

    We report on numerical and experimental characterization of the performance of a fiber link optimized for the delivery of sub-100-fs laser pulses at 1550 nm over several meters of fiber. We investigate the power handling capacity of the link, and demonstrate all-fiber delivery of 1-nJ pulses over a distance of 5.3 m. The fiber link consists of dispersion-compensating fiber (DCF) and standard single-mode fiber. The optical pulses at different positions in the fiber link are measured using frequency-resolved optical gating (FROG). The results are compared with numerical simulations of the pulse propagation based on the generalized nonlinear Schrödinger equation. The high input power capacity of the fiber link allows the splitting and distribution of femtosecond pulses to an array of fibers with applications in multi-channel fiber-coupled terahertz time-domain spectroscopy and imaging systems. We demonstrate THz pulse generation and detection using a distributed fiber link with 32 channels and 2.6 nJ input pulse energy.

  13. Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp. NJ298 in low temperature

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.

  14. 33 CFR 110.87 - Henderson Harbor, N.Y.

    Science.gov (United States)

    2010-07-01

    ... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.87 Henderson Harbor, N.Y. (a) Area A. The area in the... latitude 43°51′08.8″ N, longitude 76°12′08.9″ W, thence to latitude 43°51′09.0″ N, longitude 76°12′19.0″ W, thence to latitude 43°51′33.4″ N, longitude 76°12′19.0″ W, thence to latitude 43°51′33.4″ N, longitude...

  15. NY-ESO-1 — EDRN Public Portal

    Science.gov (United States)

    NY-ESO-1, official name CTAG1B, is an antigen that is expressed in normal testis and ovary and overexpressed in a wide variety of cancers. The CTAG1B gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence. CTAG1B is expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).

  16. Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

    OpenAIRE

    Mara Giavina-Bianchi; Pedro Giavina-Bianchi; Mirian Nacagami Sotto; Alona Muzikansky; Jorge Kalil; Cyro Festa-Neto; Duncan, Lyn M.

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. N...

  17. Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

    Directory of Open Access Journals (Sweden)

    Mara Giavina-Bianchi

    2015-01-01

    Full Text Available NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79, rarely in in situ melanoma (1/10 and not in benign nevi (0/20. Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P=0.007 and inversely correlated with superficial spreading melanoma (P<0.02. NY-ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P=0.017. When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P=0.010 or as isolated cells (P=0.002 than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  18. NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses.

    Science.gov (United States)

    Wadle, Andreas; Mischo, Axel; Strahl, Sabine; Nishikawa, Hiroyoshi; Held, Gerhard; Neumann, Frank; Wullner, Beate; Fischer, Eliane; Kleber, Sascha; Karbach, Julia; Jager, Elke; Shiku, Hiroshi; Odunsi, Kunle; Shrikant, Protul A; Knuth, Alexander; Cerundolo, Vincenzo; Renner, Christoph

    2010-11-01

    Vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. Here we report that the genetically engineered yeast Saccharomyces cerevisiae, expressing the full-length tumour-associated antigen NY-ESO-1, is a versatile host for protein production. Exposing dendritic cells (DCs) to soluble NY-ESO-1 protein linked to the yeast a-agglutinin 2 protein (Aga2p) protein resulted in protein uptake, processing and MHC class I cross-presentation of NY-ESO-1-derived peptides. The process of antigen uptake and cross-presentation was dependent on the glycosylation pattern of NY-ESO-1-Aga2p protein and the presence of accessible mannose receptors. In addition, NY-ESO-1-Aga2p protein uptake by dendritic cells resulted in recognition by HLA-DP4 NY-ESO-1-specific CD4(+) T cells, indicating MHC class II presentation. Finally, vaccination of mice with yeast-derived NY-ESO-1-Aga2p protein led to an enhanced humoral and cellular immune response, when compared to the bacterially expressed NY-ESO-1 protein. Together, these data demonstrate that yeast-derived full-length NY-ESO-1-Aga2p protein is processed and presented efficiently by MHC class I and II complexes and warrants clinical trials to determine the potential value of S. cerevisiae as a host for cancer vaccine development. Copyright © 2010 John Wiley & Sons, Ltd.

  19. NY-ESO-1 expression in synovial sarcoma and other mesenchymal tumors: significance for NY-ESO-1-based targeted therapy and differential diagnosis.

    Science.gov (United States)

    Lai, Jin-Ping; Robbins, Paul F; Raffeld, Mark; Aung, Phyu Phyu; Tsokos, Maria; Rosenberg, Steven A; Miettinen, Markku M; Lee, Chyi-Chia Richard

    2012-06-01

    A promising targeted therapy against NY-ESO-1 (CTAG 1B) using genetically modified T-cells in synovial sarcomas was recently demonstrated in a clinical trial at the NCI. To investigate the role of NY-ESO-1 immunohistochemistry in patient selection and gain better insight into the incidence of NY-ESO-1 expression in synovial sarcomas and other mesenchymal tumors, we evaluated NY-ESO-1 expression by immunohistochemistry in 417 tumors. This collection of samples included: 50 SS18/SSX1/2 fusion positive synovial sarcomas, 155 gastrointestinal stromal tumors (GIST), 135 other spindle cell sarcomas as well as 77 other sarcomas (chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, rhabdomyosarcoma, angiosarcoma, malignant mesothelioma, and Ewing's sarcoma). We report that 76% of synovial sarcomas expressed NY-ESO-1 in a strong and diffuse pattern (2-3+, >50-70% of tumor cells). In contrast, only rare cases of other spindle cell mesenchymal tumor expressed NY-ESO-1 (GIST (2/155), malignant peripheral nerve sheath tumors (1/34), and dermatofibrosarcoma protuberans (2/20)). Individual cases of other sarcomas (angiosarcoma, malignant mesothelioma, chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, and Ewing's sarcoma) were positive for NY-ESO-1. However, no positive cases were identified amongst our cohort of leiomyosarcomas (0/24), hemangiopericytoma/solitary fibrous tumors (0/40), and cellular schwannomas (0/17). In summary, we find that NY-ESO-1 is strongly and diffusely expressed in a majority of synovial sarcomas, but only rarely in other mesenchymal lesions. Beyond its role in patient selection for targeted therapy, immunohistochemistry for NY-ESO-1 may be diagnostically useful for the distinction of synovial sarcoma from other spindle cell neoplasms.

  20. Atmospheric Research in Ny-Aalesund. Proceedings from the Third NySMAC meeting. NILU, Kjeller, Norway, 9.-11. April 1997

    Energy Technology Data Exchange (ETDEWEB)

    Floeisand, Inga; Gernandt, Hartwig; Larsen, Elisabeth Stoltz; Stordal, Frode; Wada, Makoto

    1997-07-01

    Leading abstract. The Ny-Aalesund Seminar Series was conceived by NySMAC (Ny-Aalesund Science Managers Committee) as a means of bringing together research scientists involved in programmes running at Ny-Aalesund or having an interest in that area. The first Seminar was held in Potsdam in Germany in May 1995, and included talks which covered all current research disciplines; physical, atmospheric, earth and biological sciences. The Second Seminar was devoted to biological science and particularly to ecology. It was held in Cambridge in UK in February 1996. The subject of this Third Seminar, held in Kjeller in Norway in April 1997, is atmospheric research in Ny-Aalesund. Since the early 1980s a wide range of projects have been carried out on Svalbard and particularly at Ny-Aalesund. Prominent in this research have been several international research programmes with emphasis on topics of e.g. Arctic (Arctic Monitoring and Assessment Programme, AMAP), European (European Monitoring and Evaluation Programme, EMEP) or global (International Geosphere-Biosphere Programme, IGBP; World Climate Research Programme, WCRP) scales. Atmospheric observations have been carried out in Ny-Aalesund in Svalbard for several years. From the early 1980s, the long range transport of sulphur dioxide and sulphate aerosols leading to Arctic Haze, has been studied. As a consequence, EMEP included Ny-Aalesund in their network. In the following years the transport of ozone and chemical precursors were observed, and recently the naturally occurring phenomenon of rapid surface ozone loss has been given particular attention. From the late 1980s observations of the stratospheric ozone layer and related chemical species were undertaken, and the efforts have increased considerably since. Ny-Aalesund is now one of the primary Arctic sites of the NDSC (Network for Detection of Stratospheric Change) network. Important efforts have been undertaken to participate in the European campaigns as the European

  1. NY-ESO-1 expression predicts an aggressive phenotype of ovarian cancer.

    Science.gov (United States)

    Szender, J Brian; Papanicolau-Sengos, Antonios; Eng, Kevin H; Miliotto, Anthony J; Lugade, Amit A; Gnjatic, Sacha; Matsuzaki, Junko; Morrison, Carl D; Odunsi, Kunle

    2017-06-01

    NY-ESO-1 is a cancer testis antigen and a promising target for immunotherapy. The purpose of this study was to determine the expression frequency, immunogenicity, and clinical impact of NY-ESO-1 in ovarian cancer. Immunohistochemistry (IHC), reverse-transcription polymerase chain reaction (RT-PCR), and quantitative-PCR (qRT-PCR) were utilized in an ovarian cancer (including Fallopian tube and primary peritoneal cancers) patient cohort; humoral responses against NY-ESO-1 were determined by ELISA. Clinicopathologic outcomes including progression-free (PFS) and overall (OS) survival were evaluated based on NY-ESO-1 expression. Cohen's kappa (κ) tested agreement between expression tests. NY-ESO-1 expression was detected by any method in 40.7% of 1002 patients' tumors (NY-ESO-1+) and baseline humoral response was identified in 19.0% of 689 tested patients. NY-ESO-1+ patients were older (pESO-1+ patients. A subset analysis of NY-ESO-1+ patients that received immunotherapy demonstrated improved OS by >2years (52.6 vs. 27.2months, pESO-1 expression and an aggressive cancer phenotype. The relatively high expression frequency of NY-ESO-1 in ovarian cancer patients coupled with the poor clinical outcomes in NY-ESO-1+ patients reveals an underappreciated need for targeted therapy against this antigen. In support, our study reveals that NY-ESO-1+ patients enrolled on immunotherapy trials targeting the antigen exhibited an improvement in OS. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. Archive of Datasonics SIS-1000 Chirp Subbottom Data Collected During USGS Cruise DIAN 96040 Long Island, NY Inner Shelf -- Fire Island, NY, 4-24 September, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 96040 cruise. The coverage is the nearshore of Long Island, NY in...

  3. Archive of Datasonics SIS-1000 Chirp Subbottom Data Collected During USGS Cruise DIAN 96040 Long Island, NY Inner Shelf -- Fire Island, NY, 4-24 September, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 96040 cruise. The coverage is the nearshore of Long Island, NY in...

  4. NY-ESO-1抗原与肿瘤免疫治疗进展%NY-ESO-1 and Cancer Immunotherapy

    Institute of Scientific and Technical Information of China (English)

    彭吉润; 冷希圣

    2008-01-01

    NY-ESO-1抗原是肿瘤-睾丸抗原家族中的重要成员,在多种类型的肿瘤中广泛表达,并且是迄今发现的最具免疫原性的肿瘤抗原,可以在NY-ESO-1表达阳性的肿瘤患者体内引起自发性体液免疫反应和特异性T细胞免疫反应.因此,NY-ESO-1被认为是最好的肿瘤候选疫苗,在肿瘤免疫治疗中最具发展潜力.本文主要介绍了近年来有关NY-ESO-1抗原以及相关疫苗的研究进展.

  5. Progress in NY-ESO-I as cancer vaccines%NY-ESO-1癌症疫苗研究进展

    Institute of Scientific and Technical Information of China (English)

    刘志青

    2006-01-01

    NY-ESO-1抗原是近年来发现的一种肿瘤特异性抗原,在乳腺癌、黑色素瘤、食管癌等20多种肿瘤中均有不同程度的表达.NY-ESO-1的广泛表达使其成为多种实体瘤特异性疫苗免疫治疗的首选抗原.在制备NY-ESO-1癌症疫苗时,目前主要围绕癌症疫苗的类型、抗原的加工提呈两个方面进行研究.现综述近年来NY-ESO-1癌症疫苗在这两方面的研究进展.

  6. Archive of Datasonics SIS-1000 Chirp Subbottom Data Collected During USGS Cruise DIAN 96040 Long Island, NY Inner Shelf -- Fire Island, NY, 4-24 September, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 96040 cruise. The coverage is the nearshore of Long Island, NY...

  7. Induction of immune response against NY-ESO-1 by CHP-NY-ESO-1 vaccination and immune regulation in a melanoma patient.

    Science.gov (United States)

    Tsuji, Kazuhide; Hamada, Toshitada; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Isobe, Midori; Asagoe, Kenji; Yamasaki, Osamu; Shiku, Hiroshi; Ritter, Gerd; Murphy, Roger; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi; Iwatsuki, Keiji

    2008-10-01

    NY-ESO-1 is a cancer/testis antigen highly immunogenic in cancer patients. Cholesterol-bearing hydrophobized pullulan (CHP) is a nanoparticle-forming antigen-delivery vehicle and CHP complexed with NY-ESO-1 protein (CHP-NY-ESO-1) efficiently activates CD4 and CD8 T cells in vitro. In this study we report on a 50-year-old male melanoma patient with multiple skin and organ metastases (T4N3M1c) who was vaccinated with CHP-NY-ESO-1 at biweekly intervals and who had an unusual disease course. We characterized in this patient humoral and cellular immune responses, immune regulatory cells, and cytokine profiles in the peripheral blood and at local tumor sites. Ten days after the second CHP-NY-ESO-1 vaccination (day 25), blisters appeared on the skin at the metastatic lesions associated with inflammatory changes. A skin biopsy showed the presence of many NY-ESO-1-expressing apoptotic melanoma cells as determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) test. However, the tumors continued to grow, and the patient died of pulmonary failure due to multiple metastases on day 48. Serum antibody responses were detected after the second CHP-NY-ESO-1 vaccination and antibody titer increased with subsequent vaccinations. Th1 dependent IgG1 was the predominant immunoglobulin subtype. Both, NY-ESO-1-specific CD4 and CD8 T cell responses were detected in PBMC by IFN-gamma secretion assays. After CHP-NY-ESO-1 vaccination a slight decrease in CD4(+)CD25(+)Foxp3(+) Tregs was observed in PBMC but significantly increased numbers of CD4(+)CD25(+)Foxp3(+) Tregs and CD68(+) immunoregulatory macrophages were detected at the local tumor sites. CD4(+)CD25(+)Foxp3(+) Tregs were also increased in the blister fluid. Cytokines in the serum suggested a polarization towards a Th1 pattern in the PBMC and those in the blister fluid suggested a Th2-type response at the tumor site. Our observations indicate induction of specific humoral and

  8. Wind-blown sand movement periods on the Nyírség alluvial fan, Hungary

    Science.gov (United States)

    Buró, Botond; Lóki, József; Sipos, György; Négyesi, Gábor; Andrási, Bence; Jakab, Attila; Félegyházi, Enikő; Molnár, Mihály

    2017-04-01

    Wind-blown sand movement periods on the Nyírség alluvial fan, Hungary The Nyírség is an alluvial fan, was built by rivers, which were flown down from the NE Carpathians. When the weather was dry, wind-blown sand was blowed out and the wind started to develop the aeolian landforms. The first significant sand movements in the Nyírség was in the Upper plenniglacial and the Late glacial. The main landforms of the Nyírség were developed at this time. The aeolic transformation of the land was not completed in the Nyírség at the end of the Pleistocene. In the Holocene the sand moved within small area, mainly by anthropogenic impact. Our aim is clarify the age of the wind-blown sand movements period, with different absolute (Radiocarbon dating method, OSL), and relative (Archaeological finds, Pollen analyses) dating methods. We have collected for the age determining charcoal from many sand quarry (Gégény, Kántorjánosi, Nyíradony, Nagyvarsány, Máriapócs and Lövőpetri) which contain fossil soil layer. For the OSL measurements samples were collected from Baktalórántháza, Gégény and Kántorjánosi sand quarries. We collected samples from 5 places for the pollen analyses (Nyírtanya, Máriapócs, Nyírbéltek-Nyírlúgos, Nyírábrány, Vámospércs), and also from an archaeological excavation (Nyíregyháza- Oros). The new age dates show us, that in the Nyírség the first significant sand movements were in the cold and drier period of the Upper pleniglacial and Late glacial (Baktalórántháza, Nyírtanya, Vámospércs). At the end of the Pleistocene the sand movements not competed in the Nyírség (Gégény, Kántorjánosi, Nyírábrány). In the Holocene there were many little sand movement periods, mainly caused by anthropogenic impact (Nyíradony, Nyíregyháza-Oros). The research was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund in the project of GINOP-2.3.2.-15-2016-00009 'ICER

  9. RoMo

    DEFF Research Database (Denmark)

    Pedersen, Esben Warming; Hornbæk, Kasper

    2011-01-01

    In TUIs, physical/digital conflicts can occur when the digital model does not match the model implied by the spatial lay- out of tangibles. We show how tangible tabletop interfaces (TTI) can be modified to allow robot movement of tangi- bles, thereby avoiding conflicts. We present RoMo, an open...

  10. 33 CFR 110.150 - Block Island Sound, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Block Island Sound, N.Y. 110.150 Section 110.150 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A...

  11. 33 CFR 110.85 - Niagara River, Youngstown, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Niagara River, Youngstown, N.Y. 110.85 Section 110.85 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.85 Niagara River, Youngstown, N.Y. (a) Area...

  12. 78 FR 35596 - Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY

    Science.gov (United States)

    2013-06-13

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY AGENCY: Coast Guard, DHS. ACTION: Notice of Proposed Rulemaking... Atlantic Ocean off Long Beach, NY during the Long Beach Regatta Powerboat Race scheduled for August...

  13. Epigenetic potentiation of NY-ESO-1 vaccine therapy in human ovarian cancer.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; James, Smitha R; Mhawech-Fauceglia, Paulette; Tsuji, Takemasa; Miller, Austin; Zhang, Wa; Akers, Stacey N; Griffiths, Elizabeth A; Miliotto, Anthony; Beck, Amy; Batt, Carl A; Ritter, Gerd; Lele, Shashikant; Gnjatic, Sacha; Karpf, Adam R

    2014-01-01

    The cancer-testis/cancer-germline antigen NY-ESO-1 is a vaccine target in epithelial ovarian cancer (EOC), but its limited expression is a barrier to vaccine efficacy. As NY-ESO-1 is regulated by DNA methylation, we hypothesized that DNA methyltransferase (DNMT) inhibitors may augment NY-ESO-1 vaccine therapy. In agreement, global DNA hypomethylation in EOC was associated with the presence of circulating antibodies to NY-ESO-1. Pre-clinical studies using EOC cell lines showed that decitabine treatment enhanced both NY-ESO-1 expression and NY-ESO-1-specific CTL-mediated responses. Based on these observations, we performed a phase I dose-escalation trial of decitabine, as an addition to NY-ESO-1 vaccine and doxorubicin liposome (doxorubicin) chemotherapy, in 12 patients with relapsed EOC. The regimen was safe, with limited and clinically manageable toxicities. Both global and promoter-specific DNA hypomethylation occurred in blood and circulating DNAs, the latter of which may reflect tumor cell responses. Increased NY-ESO-1 serum antibodies and T cell responses were observed in the majority of patients, and antibody spreading to additional tumor antigens was also observed. Finally, disease stabilization or partial clinical response occurred in 6/10 evaluable patients. Based on these encouraging results, evaluation of similar combinatorial chemo-immunotherapy regimens in EOC and other tumor types is warranted.

  14. Expression and immunogenicity of NY-ESO-1 in colorectal cancer.

    Science.gov (United States)

    Li, Ya; Song, Ruifeng; Li, Xinqiang; Xu, Feng

    2017-06-01

    Serum assays of NY-ESO-1 antibodies provide a guide to discriminate between patients who suffer from different types of cancer. In the present study, the expression of NY-ESO-1 was detected with the aim to identify a novel tumor antigen in colorectal cancer (CRC). Sera were obtained from 89 healthy individuals and 236 patients with CRC with stage I, II, III and IV tumors. The NY-ESO-1 autoantibody levels were determined using an enzyme-linked immunosorbent assay. The mRNA and protein expression levels of NY-ESO-1 were detected using reverse transcription-polymerase chain reaction and immunohistochemistry, respectively, in 60 CRC and paired adjacent non-tumor tissues. NY-ESO-1 antibody was detected in 40 of the 236 (16.9%) patients with CRC. The NY-ESO-1 antibody combined with carcinoembryonic antigen enhanced the sensitivity, from 52.1 to 62.7%, of the diagnosis of CRC. The frequency of antibody positivity increased with the TNM cancer stage (8.8 vs. 28.3% in stages I+II and III+IV, respectively). The mRNA and protein expression levels of NY-ESO-1 were significantly higher in CRC tissue than in adjacent non-tumor tissue. In conclusion, NY-ESO-1 is frequently expressed in CRC with the capacity of inducing a humoral immune response in CRC patients, exhibiting the potential to be a promising biomarker for CRC.

  15. Increased NY-ESO-1 expression and reduced infiltrating CD3+ T cells in cutaneous melanoma.

    Science.gov (United States)

    Giavina-Bianchi, Mara; Giavina-Bianchi, Pedro; Sotto, Mirian Nacagami; Muzikansky, Alona; Kalil, Jorge; Festa-Neto, Cyro; Duncan, Lyn M

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P = 0.007) and inversely correlated with superficial spreading melanoma (P ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P = 0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P = 0.010) or as isolated cells (P = 0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  16. NY-ESO-1-specific immunological pressure and escape in a patient with metastatic melanoma.

    Science.gov (United States)

    von Boehmer, Lotta; Mattle, Muriel; Bode, Peter; Landshammer, Alexandro; Schäfer, Carolin; Nuber, Natko; Ritter, Gerd; Old, Lloyd; Moch, Holger; Schäfer, Niklaus; Jäger, Elke; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    During cancer progression, malignant cells may evade immunosurveillance. However, evidence for immunological escape in humans is scarce. We report here the clinical course of a melanoma patient whose initial tumor was positive for the antigens NY-ESO-1, MAGE-C1, and Melan-A. Upon immunization with a recombinant vaccinia/fowlpox NY-ESO-1 construct, the patient experienced a mixed clinical response and spreading of the NY-ESO-1 epitopes in the CD4+ T cell compartment. After NY-ESO-1 protein + CpG immunization, the patient's anti-NY-ESO-1 IgG response increased. Over the following years, progressing lesions were resected and found to be NY-ESO-1-negative while being positive for MAGE-C1, Melan-A, and MHC-I. The fatal, inoperable brain metastasis was analyzed after his death and also proved to be NY-ESO-1-negative, while being positive for MAGE-C1 and Melan-A, as well as MHC-I. We propose that cancer control and cancer escape in this patient were governed by NY-ESO-1-specific immunological pressure. Our findings provide evidence for the existence of immunoediting and immunoescape in this cancer patient.

  17. 75 FR 38710 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-07-06

    ... Championships, Huntington Bay, NY'' in the Federal Register (75 FR 13454). The Coast Guard received no comments...-2009-0520) in the Federal Register (74 FR 51243). The notice proposed a regulated area encompassing 100... Bay, NY in the Federal Register (75 FR 13454). This notice proposes a 100 yard regulated area...

  18. 77 FR 35113 - Canadian National Railway Company-Abandonment Exemption-in Niagara County, NY

    Science.gov (United States)

    2012-06-12

    ... milepost 0.20 to approximately milepost 0.35 in the City of Niagara Falls, Niagara County, N.Y., a distance... track in Niagara Falls, extending between the eastern end of the Bridge and the beginning of the Niagara... Niagara Falls, N.Y., and Niagara Falls, Ontario, Canada, and is owned by the bi-national Niagara......

  19. 33 CFR 165.170 - Safety Zone: Triathlon, Ulster Landing, Hudson River, NY.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone: Triathlon, Ulster Landing, Hudson River, NY. 165.170 Section 165.170 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.170 Safety Zone: Triathlon, Ulster Landing, Hudson River, NY. (a) Regulated area. The...

  20. 77 FR 30451 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

    Science.gov (United States)

    2012-05-23

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY... establish a temporary safety zone on Lake Ontario, Olcott, NY. This proposed rule is intended to restrict vessels from a portion of Lake Ontario during the Olcott fireworks display. The safety zone established...

  1. Isolation and optimization of production of Astaxanthin from Antarctic yeast Rhodotorula sp.NJ298

    Institute of Scientific and Technical Information of China (English)

    Liu Junling; Miao Jinlai; Sun Xiuqin; Wang Quanfu; Li Guangyou

    2007-01-01

    Rhodotorula sp. NJ298 which could produce carotenoids was isolated from Antarctic sea ice. The major carotenoid was identified as astaxanthin by Liquid Chromatography/Mass Spectrometry(LC/MS), and its content accounted for 87.62% of total carotenoids (1,786 μg/g). High Performance Liquid Chromatogrephy(HPLC)analysis showed that the purity of the astaxanthin reached about 96.16% through a simple purification. Maximum astaxanthin production(1,908/μg/g)was obtained when the yeast was grown at 10℃ in seawater medium containing 5g/L sodium acetate, 5g/L peptone, 0.5g/L NaCl, 0.01g/L KH2P04;0.01g/L MgS04·7H2O and 0.001 g/L FeS04·7H20 at pH 7.5.

  2. Do Affordable Housing Projects Harm Suburban Communities? Crime, Property Values, and Taxes in Mount Laurel, NJ.

    Science.gov (United States)

    Albright, Len; Derickson, Elizabeth S; Massey, Douglas S

    2013-06-01

    This paper offers a mixed-method analysis of the municipal-level consequences of an affordable housing development built in suburban New Jersey. Opponents of affordable housing development often suggest that creating affordable housing will harm surrounding communities. Feared consequences include increases in crime, declining property values, and rising taxes. To evaluate these claims, the paper uses the case of Mt. Laurel, NJ - the site of a landmark affordable housing legal case and subsequent affordable housing development. Employing a multiple time series group control design, we compare crime rates, property values, and property taxes in Mt. Laurel to outcomes in similar nearby municipalities that do not contain comparable affordable housing developments. We find that the opening of the affordable housing development was not associated with trends in crime, property values, or taxes, and discuss management practices and design features that may have mitigated potential negative externalities.

  3. Optical fiber link for transmission of 1-nJ femtosecond laser pulses at 1550 nm

    DEFF Research Database (Denmark)

    Eichhorn, Finn; Olsson, Rasmus Kjelsmark; Buron, Jonas Christian Due

    2010-01-01

    We report on numerical and experimental characterization of the performance of a fiber link optimized for the delivery of sub-100-fs laser pulses at 1550 nm over several meters of fiber. We investigate the power handling capacity of the link, and demonstrate all-fiber delivery of 1-nJ pulses over...... a distance of 5.3 m. The fiber link consists of dispersion-compensating fiber (DCF) and standard single-mode fiber. The optical pulses at different positions in the fiber link are measured using frequency-resolved optical gating (FROG). The results are compared with numerical simulations of the pulse...... propagation based on the generalized nonlinear Schrödinger equation. The high input power capacity of the fiber link allows the splitting and distribution of femtosecond pulses to an array of fibers with applications in multi-channel fiber-coupled terahertz time-domain spectroscopy and imaging systems. We...

  4. Effects of Temperature and Several Chemicals on Metabolic Changes During Dormancy Release in NJ72 Nectarine

    Institute of Scientific and Technical Information of China (English)

    YUAN Zhi-you; LI Xian-li; LI Ling-hao; HAN Xing-guo; YAN Tian-li

    2003-01-01

    Poor, delayed and ununiform budbreak is a major problem for peaches in greenhouse. To clari-fy the mechanism of breaking bud dormancy in nectarines, the effect of temperature and three dormancy-breaking agents on metabolic changes during dormancy release in two-year old NJ72 nectarine (Prunus persicaL. Batch) was investigated. The result showed temperature and chemicals affected the budbreak and the me-tabolism of NJ72 nectarine during dormancy. Endogeneons peroxide content in buds increased soon after lowtemperature treatment. Meanwhile, catalase activity was also shown to increase significantly at low temperature treatment, coincided with increase of the activity of peroxidase and superoxide dismntase. The rate of res-piration in flower buds increased at low temperature during dormancy. The rate of the pentose phosphate path-way increased, while the rate of the Embden-Meyerhof pathway decreased and the rate of triearboxlic acid cycle changed little. Glucose 6-phosphate dehydrogenase activity increased at low temperature during dormancy.At the same time we found an accumulation of peroxide after treatment with dormancy-breaking chemicals. Inflower buds treated with dormancy-breaking agents, thiourca, KNO3 and NH4 NO3, catalase activity was in-hibited soon after treatment, whereas peroxidase activity increased, and the changes of superoxide dismutaseremained little. In this study, it was found that the rates of respiration in flower buds increased by chemicalssprays during dormancy. The activity of glucose 6-phosphate dehydrogenase, the key enzyme in the pentosephosphate pathway (PPP), increased by spraying with dormancy-breaking agents, concomitantly with the ac-tivatlon of the pentose phosphate pathway.

  5. 77 FR 3499 - Sony Electronics, Inc., Including On-Site Leased Workers From Selectremedy Park Ridge, NJ...

    Science.gov (United States)

    2012-01-24

    ...] Sony Electronics, Inc., Including On-Site Leased Workers From Selectremedy Park Ridge, NJ; Amended... of Sony Electronics, Inc., SEL Headquarters, including on-site leased workers of SelectRemedy, StaffMark, and Payrolling.com , San Diego, California (TA-W-71,501); Sony Electronics, Inc., including...

  6. Constructing A New Japanese Development Design Model NJ-DDM: Intellectual Evolution of an Automobile Product Design

    Directory of Open Access Journals (Sweden)

    Kakuro Amasaka

    2015-11-01

    Full Text Available The author constructs a New Japanese Development Design Model “NJ-DDM” utilizing CAE to realize “simultaneous achievement of QCD” (quality, cost and delivery in automobile development design by evolving the super-short-term development design process. Based on this, the author verifies the validity of this model in intellectual evolution of an automobileproduct design.

  7. U.S. EPA’s Technical Support for the Reich Farm (Toms River, NJ) Superfund Site Remediation

    Science.gov (United States)

    PowerPoint slide file that gives a brief history of the SAN Trimer contamination in Toms River, NJ as well as the EPA's provided technical support, specifically the development and application of the Provisional Peer-Reviewed Toxicity Value (PPRTV) assessment for SAN Trimer.

  8. 77 FR 34285 - Safety Zone; 2012 Ironman U.S. Championship Swim, Hudson River, Fort Lee, NJ

    Science.gov (United States)

    2012-06-11

    ... the January 17, 2008, issue of the Federal Register (73 FR 3316). Public Meeting We do not plan now to... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; 2012 Ironman U.S. Championship Swim, Hudson... vicinity of Englewood Cliffs and Fort Lee, NJ for the 2012 Ironman U.S. Championship swim event....

  9. Bacterial Diversity of Ny-Ålesund, Arctic Archipelago Svalbard

    Directory of Open Access Journals (Sweden)

    Battsetseg Choidash

    2012-12-01

    Full Text Available The bacterial diversity of the water sample, collected from Ny-Ålesund, Arctic Archipelago Svalbard was analyzed by a phenotypic as well as a genotypic approach. Pure colonies of the culturable bacteria were established and grown at a range of temperatures: 4ºC, 15ºC, 22ºC and 37ºC. Optimum growth was found at 15ºC, and around 28 colonies were obtained. The library was dominated by 16S rDNAs of Gram-negative bacteria ( γ -Proteobacteria. Twenty two isolates exhibited sequences were similar to that of known bacterial isolates (>97% sequence similarity, represented by the species of the genera Psychrobacter , Pseudomonas , and Acinetobacter . Six isolates exhibited sequences showed less affi liation with known taxa (<97% sequence similarity, and may represent novel taxa.

  10. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

    Science.gov (United States)

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H; Kalos, Michael D; Grupp, Stephan A

    The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastoma samples and demonstrated that 23% express NY-ESO-1. After confirming antigen-specific activity of T cells genetically engineered to express an NY-ESO-1 directed high-affinity transgenic T cell receptor in vitro, we performed xenograft mouse studies assessing the efficacy of NY-ESO-1-targeted T cells in both localized and disseminated models of neuroblastoma. Disease responses were monitored by tumor volume measurement and in vivo bioluminescence. After delivery of NY-ESO-1 transgenic TCR T cells, we observed significant delay of tumor progression in mice bearing localized and disseminated neuroblastoma, as well as enhanced animal survival. These data demonstrate that NY-ESO-1 is an antigen target in neuroblastoma and that targeted T cells represent a potential therapeutic option for patients with neuroblastoma.

  11. Expression of MAGE-A and NY-ESO-1 in Primary and Metastatic Cancers.

    Science.gov (United States)

    Park, Tristen S; Groh, Eric M; Patel, Krishna; Kerkar, Sid P; Lee, Chyi-Chia Richard; Rosenberg, Steven A

    2016-01-01

    Melanoma-associated antigen-A (MAGE-A) and New York esophageal squamous cell cancer-1 (NY-ESO-1) are 2 cancer testis antigens (CTA) demonstrating potential for use in targeted immunotherapy. Clinical trials in melanoma and synovial sarcomas targeting these antigens in immune-based therapies have demonstrated durable tumor regression. Although protein expression of NY-ESO-1 has been assessed in a variety of cancer types, the expression of MAGE-A has not been studied in depth. In this study we analyzed MAGE-A and NY-ESO-1 expression in 314 melanoma specimens from 301 melanoma patients, 38 patients with squamous cell cancers and 111 patients with adenocarcinomas. Our results demonstrated higher expression of MAGE-A compared with NY-ESO-1 in melanomas (32% vs. 13%) and squamous cell carcinomas (45% vs. 7.9%), and higher expression of both CTAs in metastatic versus primary tumors. CTA expression in adenocarcinomas was low (MAGE-A: 10%, NY-ESO-1: 0.9%). In addition, we looked at concordance of expression among metastatic melanoma lesions within the same patient and found concordant expression in 38 of 47 patients for MAGE-A and 43 of 47 patients for NY-ESO-1. Our study demonstrated that the MAGE-A family may be of greater utility than NY-ESO-1 for targeted immunotherapy in a variety of cancer histologies, in particular metastatic melanomas and squamous cell carcinomas.

  12. Expression of NY-ESO-1 ,NY-SAR-35 in retinoblastoma and its clinical significance%视网膜母细胞瘤中NY-ESO-1、NY-SAR-35基因的表达及其临床意义

    Institute of Scientific and Technical Information of China (English)

    严宇清; 林泉; 孔旻; 何剑峰; 陈迎迎; 谢小薰; 罗彬; 梁皓

    2012-01-01

    背景 视网膜母细胞瘤(RB)的免疫治疗因创伤小、针对性强而受到广泛关注,寻求相关免疫原性较强的肿瘤抗原是免疫治疗的基础.NY-ESO-I和NY-SAR-35是癌-睾丸抗原(CTA)中的两种基因,检测其在RB组织中的表达可为RB的免疫治疗研究提供依据. 目的 研究RB中NY-ESO-1 mRNA、NY-SAR-35 mRNA的表达情况,探讨将其用于RB特异性免疫治疗靶抗原的可能性. 方法 收集15例RB患儿组织为RB组、12例非肿瘤病变部位视网膜组织为非肿瘤病变组,及22例眼部因其他眼部病变进行手术治疗获取的正常相关眼组织标本为正常组.采用逆转录聚合酶链反应(RT-PCR)检测待测组织标本中NY-ESO-1mRNA、NY-SAR-35 mRNA的表达;并按随机数字表法抽取RT-PCR检测的阳性产物直接进行DNA测序,对检测结果与肿瘤病理分级、肿瘤大小、临床分期等临床指标进行分析.结果 15例RB组织中有6例标本表达NY-ESO-1基因,9例标本表达NY-SAR-35基因;在12例非肿瘤病变视网膜组织及22例眼部正常组织中均未见到NY-ESO-1基因和NY-SAR-35基因的表达.NY-ESO-1基因和NY-SAR-35基因的表达均与患者的性别(P=0.426、0.822)、年龄(P=0.180、0.464)、病理分型(P=0.744、0.582)、肿瘤大小(P=0.760、0.790)、临床分期(P=0.868、0.707)等临床相关指标无明显关联.结论 NY-ESO-1基因及NY-SAR-35基因均可特异性地高表达于RB组织中,其表达率明显高于其他全身肿瘤,有可能成为RB特异性免疫治疗的靶抗原.%Background The immunotherapy for retinoblastoma(RB) is gradually concerned recent year.To seek relative immune-associated antigen is a basis of immunotherapy.NY-ESO-1 and NY-SAR-35 are two kinds of genes of cancer testis antigen( CTA ).To understand their expressions in RB tissue can offer index for relative study.Objective This study was to investigate the expressions of two CTA NY-ESO-1 and NY-SAR-35 in RB and explore the possibility of them as

  13. MO ACTUALIZAR LA DEUDA PÚBLICA INTERNA OLVIDADA POR EL GOBIERNO PERUANO

    OpenAIRE

    Avelino Sánchez, Esteban Marino; Facultad de Ciencias Contables, Universidad Nacional Mayor de San Marcos

    2015-01-01

    ¿Cómo actualizar la deuda de los Bonos de Reconstruccióny Bonos de Desarrollo emitidos por elGobierno peruano en monedas que se retiraron de circulación?,constituye el problema que en este artículotratamos de explicar. La operación actualizar requiere,previamente, discernir sobre el bien económico oindicadores referentes de valor que se aplicarán en lasoperaciones de cálculo; en ese sentido, proponemosdos procedimientos: la actualización con el dólarestadounidense, dolarizando la deuda en la ...

  14. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy.

    Science.gov (United States)

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1(157-165)/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer.

  15. Application of serum NY-ESO-1 antibody assay for early SCLC diagnosis.

    Science.gov (United States)

    Yang, Jihua; Jiao, Shunchang; Kang, Jingbo; Li, Rong; Zhang, Guanzhong

    2015-01-01

    NY-ESO-1 antibody is one of the cancer-related antibodies. The purpose of this study was to investigate the diagnostic role of the NY-ESO-1 humoral immune response in small cell lung cancer (SCLC). We recombined the recombinant protein of NY-ESO-1 antibody and NSE, analyzed them by Enzyme-linked immunosorbent assay, and then established the Receiver Operating Characteristic (ROC) curve to estimate the diagnostic value of NY-ESO-1 antibody, NSE and their combinations. According to detection, the positive rate of NY-ESO-1 humoral immune response (26.3%), NSE (43.8%) and their combinations (10.5%) were all lower than the negative rate which indicated that the NY-ESO-1 antibody might be down-regulated in SCLC. And the positive rate wasn't related to clinicopathologic characteristics. The ROC curve demonstrated that with a 37.17% sensitivity and a 91.7% specificity along with a AUC of 0.619 for NY-ESO-1ab as well as with a 48.3% sensitivity and a 90.87% specificity along with a AUC value of 0.773 for NSE, their diagnostic value were both high. Besides, the diagnostic value of their combinations was also good for a AUC of 0.83 and a 69.12% sensitivity and a 91.8% specificity. There were significant difference of diagnostic value among three types above (NY-ESO-1 vs. NSE, P ESO-1, P ESO-1ab, NSE and their combinations all were important diagnostic markers for SCLC. Moreover, the diagnostic value of their combinations was higher than any single of them. And NY-ESO-1 humoral immune to NSE might be a potential diagnostic indicator in SCLC.

  16. Expression of NY-ESO-1 gene in human hepatocellular carcinoma%人类NY-ESO-1基因编码在肝癌中的表达

    Institute of Scientific and Technical Information of China (English)

    张阳德; 任菲; 彭健

    2013-01-01

    目的 研究NY-ESO-1在肝细胞肝癌中的表达情况并探索其表达与肝癌预后的相关性.方法 我们收集了124例肝癌患者的肝癌组织和相应的癌旁组织,37例肝硬化患者的肝组织,以及43例非肝硬化的肝组织.应用实时荧光定量聚合酶链技术(RT-PCR)检测了上述组织中NY-ESO-1基因的表达水平,并利用DNA免疫杂交技术和测序技术检测RT-PCR产物.收集健康志愿者外周血基因组DNA作为正常对照.结果 在124例肝癌组织中,56例(45.2%)检测到有NY-ESO-1基因的表达,而在癌旁组织中未检测到表达.NY-ESO-1基因的RT-PCR产物长度为329 bp,利用测序方法检测NY-ESO-1基因突变.在56例表达NY-ESO-1的肝癌组织中,有4例检测到位点2的突变.该突变导致一个氨基酸的变异,这种氨基酸的变异对蛋白结构和功能具有决定性的影响.NY-ESO-1基因的表达与临床特征如肿瘤标志物(甲胎蛋白,半乳糖酸苷酶)、转移、复发和肿瘤的大小无显著关联性.结论 NY-ESO-1在肝癌组织中呈高表达,但是与预后无相关性.NY-ESO-1蛋白能否作为肝癌治疗的候选疫苗有待进一步研究.

  17. Hurricane Sandy puts NJ hospital under extreme stress, highlighting vulnerabilities, areas requiring improvement.

    Science.gov (United States)

    2013-01-01

    When monster storm, Hurricane Sandy, struck the northeastern coast in late October, the emergency systems for many hospitals in the region were stressed beyond their limits. At least four hospitals in the region had to be evacuated, and many hospitals lost power and access to essential services. Using backup generators, CentraState Medical Center in Freehold, NJ, was able to keep its doors open throughout the emergency, but the event highlighted a number of vulnerabilities that administrators will work to improve. Demand for care spiked because people in the hospital's service area could not get in to see their primary care providers. The hospital established care areas next to its emergency department to handle the demand, and it also enabled physicians in the region to see patients in offices on an ambulatory campus, adjacent to the hospital. Emergency department visits increased by about 41% during the hurricane week, admits went up by about 50%, and the number patients sent to observation went up by 450%, according to hospital administrators. In the future, hospital leaders say practice drills need to regularly test for events that cause many systems to go down, rather then testing for one vulnerability at a time.

  18. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery.

    Science.gov (United States)

    Xu, Heng; Gu, Na; Liu, Zhao-Bo; Zheng, Min; Xiong, Fang; Wang, Si-Ying; Li, Ning; Lu, Jun

    2012-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for this correlation. A total of 120 HCC patients were analyzed for the expression of NY-ESO-1 by immunohistochemistry (IHC). A stable NY-ESO-1 over-expressed HepG2 cell line (ESO-HepG2) was established to determine the biological effects of NY-ESO-1 on cell proliferation, cell cycle and migration by using the xCELLigence DP system, flow cytometry and xCELLigence SP system. NY-ESO-1 was positive in 28 of 120 (23.3%) HCC tumor tissues. NY-ESO-1 was not detectable in adjacent normal liver tissues. A close correlation was found between NY-ESO-1 expression and the recurrence of HCC following surgery (P=0.007). Kaplan-Meier analysis showed a shorter recurrence-free survival (RFS) for patients positive for NY-ESO-1 (log-rank test, P=0.003). The Cox regression model demonstrated that NY-ESO-1 expression was a significant independent predictor for the recurrence of HCC following curative surgery (P=0.022). Compared with HepG2 cells, ESO-HepG2 cells have increased migration but not proliferation ability. In conclusion, NY-ESO-1 expression is associated with worse HCC outcome following surgery, and the mechanism for this finding may be that NY-ESO-1 increases tumor cell migration.

  19. THERMOMECHANICAL ANALYSIS OF ILLITE FROM FÜZÉRRADVÁNY

    Directory of Open Access Journals (Sweden)

    Tomáš Húlan

    2015-09-01

    Full Text Available Young’s modulus of green illite from Füzérradvány (Hungary was measured in-situ in the temperature interval 20 °C – 1100 °C and auxiliary analyses DTA, TG and TDA, XRD and EGA, were also performed. It was found that a removal of the physically bound water (20 °C – 250 °C sets illite crystals closer which leads to a significant increase of Young’s modulus from its initial value of 7 GPa and reaches the maximum 12 GPa at 300 °C. Young’s modulus slightly decreases in the temperature interval of dehydroxylation of illite (300 °C – 800 °C, and which then reaches up to 45 GPa at 1100 °C, increasing exponentially as a consequence of the sintering above 800 °C.DOI: http://dx.doi.org/10.5755/j01.ms.21.3.7152

  20. A note on the connectedness locus of the families of polynomials Pc(z=z n - cz n-j

    Directory of Open Access Journals (Sweden)

    Carlos Arteaga

    2012-03-01

    Full Text Available Let j be a positive integer. For each integer n > j we consider the connectedness locus Mn of the family of polynomials Pc(z = z n - cz n-j, where c is a complex parameter. We prove that lim n→∞ Mn = D in the Hausdorff topology, where D is the unitary closed disk {c;|c|Seja j um inteiro positivo. Para cada inteiro n > j, consideramos o locus conexo Mn da família de polinômios Pc(z = z n - cz n-j, onde c é um parâmetro complexo. Provamos que lim n→∞ Mn = D na topologia de Hausdorff; onde D é o disco unitário {c;|c|<1}.

  1. 肿瘤抗原NY-ESO-1研究进展

    Institute of Scientific and Technical Information of China (English)

    王运伟; 顾琴龙; 朱正纲

    2005-01-01

    肿瘤抗原NY-ESO-1是肿瘤-睾丸(cancer-testis,CT)抗原,其编码基因是一基因家族,目前发现的主要包括NY-ESO-1、LAGE-1和ESO3三个成员.NY-ESO-1是迄今发现的最具免疫原性的肿瘤抗原之一,可以在NY-ESO-1表达阳性的肿瘤患者体内引起自发性体液免疫反应和T细胞免疫反应,因此在抗原特异性肿瘤免疫治疗中具有广泛的应用前景.

  2. EnviroAtlas - New York, NY - One Meter Resolution Urban Land Cover Data (2008) Web Service

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas web service supports research and online mapping activities related to EnviroAtlas (https://www.epa.gov/enviroatlas ). The New York, NY EnviroAtlas...

  3. EnviroAtlas - New York, NY - Meter-Scale Urban Land Cover (MULC) Data (2008)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The New York, NY EnviroAtlas Meter-scale Urban Land Cover (MULC) Data were generated by the University of Vermont Spatial Analysis Laboratory (SAL) under the...

  4. LiDAR Elevation Data Collection - Putnam County, NY, 2008 (NYSDEC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Summary of the surface elevation data collection project in Putnam County, NY (NYSDEC) 2008. Products generated include LiDAR point data in LAS Binary format v1.1....

  5. 76 FR 4078 - Television Broadcasting Services; North Pole and Plattsburgh, NY

    Science.gov (United States)

    2011-01-24

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; North Pole and Plattsburgh, NY AGENCY: Federal... CFR Part 73 Television, Television broadcasting. For the reasons discussed in the preamble,...

  6. EnviroAtlas - New York, NY - One Meter Resolution Urban Land Cover Data (2008)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The New York, NY EnviroAtlas Meter-scale Urban Land Cover (MULC) Data were generated by the University of Vermont Spatial Analysis Laboratory (SAL) under the...

  7. Advances in the Research of Tumor Antigen NY-ESO-1%肿瘤抗原NY-ESO-1研究进展

    Institute of Scientific and Technical Information of China (English)

    高丹

    2008-01-01

    NY-ESO-1是肿瘤-睾丸(cancer-testis,CT)抗原家族成员之一,在许多不同类型的人类肿瘤中都有表达,是迄今发现的最具免疫原性的肿瘤抗原之一.NY-ESO-1可以在阳性表达肿瘤患者体内诱导自发性体液和T细胞免疫应答,因此在肿瘤疫苗研制中具有非常广泛的前景.

  8. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma

    OpenAIRE

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M.; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H.; Kalos, Michael D.; Grupp, Stephan A.

    2015-01-01

    The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastom...

  9. NY-ESO-1 expression is tightly linked to TMPRSS2-ERG fusion in prostate cancer.

    Science.gov (United States)

    Grupp, Katharina; Ospina-Klinck, Daniel; Tsourlakis, Maria Christina; Koop, Christina; Wilczak, Waldemar; Adam, Meike; Simon, Ronald; Sauter, Guido; Izbicki, Jakob Robert; Graefen, Markus; Huland, Hartwig; Steurer, Stefan; Schlomm, Thorsten; Minner, Sarah; Quaas, Alexander

    2014-07-01

    NY-ESO-1 has been suggested as therapeutic cancer vaccine in prostate cancer. This study was undertaken to explore the relationship of NY-ESO-1 with tumor phenotype, biochemical recurrence, and molecular subgroups in hormone-naive prostate cancers. NY-ESO-1 immunohistochemistry was analyzed on a tissue microarray containing 11,152 prostate cancer samples. Results were compared to clinically follow-up data, ERG status, and deletions on PTEN, 3p13, 5q21, and 6q15. NY-ESO-1 expression was absent in benign prostate glands. In prostate cancer, NY-ESO-1 positivity was found 8.8% of our 8,761 interpretable tumors including 5.8% with weak, 2.5% with moderate, and 0.5% with strong expression. There was a threefold higher rate of NY-ESO-1 expression in ERG fusion positive tumors than in ERG negative cancers (P ESO-1 expression was associated with early biochemical recurrence (P = 0.0002) and high Gleason grade (P ESO-1 expression was also linked to PTEN (P = 0.0012) and 6q15 deletions (P = 0.0005). Our observations indicate a tight link of NY-ESO-1 expression to ERG activation and (to a lesser extent) PTEN- and 6q15-deletions in prostate cancer. The impact of these interactions on the likelihood of response to immunotherapy is unclear. The prognostic impact of NY-ESO-1 expression is little and not independent of histologic variables. © 2014 Wiley Periodicals, Inc.

  10. Diagnostic and therapeutic relevance of NY-ESO-1 expression in oral squamous cell carcinoma.

    Science.gov (United States)

    Ries, Jutta; Mollaoglu, Nur; Vairaktaris, Eleftherios; Neukam, Friedrich W; Nkenke, Emeka

    2009-12-01

    Cancer/testis antigen 1B (NY-ESO-1) is exclusively expressed in various types of tumor but not in healthy normal tissue, except testis, and induces strong cellular and humoral immune responses. Therefore, it represents an ideal target for diagnostic and immunotherapeutic applications. The aim of the study was to investigate the expression of NY-ESO-1 in oral squamous cell carcinoma (OSCC) to determine its impact as a diagnostic parameter or a therapeutic target for oral cancer. A total of 65 OSCC and 20 normal oral mucosal samples of otherwise healthy volunteers were included in this study. Expression of NY-ESO-1 was determined using reverse transcriptase polymerase chain reaction (RT-PCR). The results were correlated to diagnosis and clinicopathological parameters. NY-ESO-1 was expressed in 27.7% of the investigated tumor samples, but not in normal oral mucosal. The correlation between NY-ESO-1 expression and malignancy was significant (p=0.008). The prevalence of NY-ESO-1 expression was significantly associated with tumor size (p=0.033), but not with histological grading, positive lymph node status or clinical stage of disease. NY-ESO-1 expression is restricted to OSCC, clearly indicating malignancy. However, the expression rate of this antigen is too low for clinical application but it might be a useful additional biomarker within a multiple marker system for the diagnosis of OSCC. In addition, NY-ESO-1 might be a candidate for immunotherapy and polyvaccination in patients suffering from OSCC.

  11. Three novel NY-ESO-1 epitopes bound to DRB1*0803, DQB1*0401 and DRB1*0901 recognized by CD4 T cells from CHP-NY-ESO-1-vaccinated patients.

    Science.gov (United States)

    Mizote, Yu; Taniguchi, Taku; Tanaka, Kei; Isobe, Midori; Wada, Hisashi; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Uenaka, Akiko; Nakayama, Eiichi

    2010-07-19

    Three novel NY-ESO-1 CD4 T cell epitopes were identified using PBMC obtained from patients who were vaccinated with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1). The restriction molecules were determined by antibody blocking and using various EBV-B cells with different HLA alleles as APC to present peptides to CD4 T cells. The minimal epitope peptides were determined using various N- and C-termini truncated peptides deduced from 18-mer overlapping peptides originally identified for recognition. Those epitopes were DRB1*0901-restricted NY-ESO-1 87-100, DQB1*0401-restricted NY-ESO-1 95-107 and DRB1*0803-restricted NY-ESO-1 124-134. CD4 T cells used to determine those epitope peptides recognized EBV-B cells or DC that were treated with recombinant NY-ESO-1 protein or NY-ESO-1-expressing tumor cell lysate, suggesting that the epitope peptides are naturally processed. These CD4 T cells showed a cytokine profile with Th1 characteristics. Furthermore, NY-ESO-1 87-100 peptide/HLA-DRB1*0901 tetramer staining was observed. Multiple Th1-type CD4 T cell responses are beneficial for inducing effective anti-tumor responses after NY-ESO-1 protein vaccination. (c) 2010 Elsevier Ltd. All rights reserved.

  12. [Colonization characteristics of endophytic bacteria NJ13 in Panax ginseng and its biocontrol efficiency against Alternaria leaf spot of ginseng].

    Science.gov (United States)

    Chen, Chang-Qing; Li, Tong; Li, Xin-Lian; Jiang, Yun; Tian, Lei; Xu, Peng

    2014-05-01

    To reveal the colonization characteristics in host of endophytic biocontrol bacteria NJ13 isolated from Panax ginseng, this study obtained the marked strain NJ13-R which was double antibiotic resistant to rifampicin and streptomycin through enhancing the method of inducing antibiotic. The colonization characteristics in ginseng and its biocontrol efficiency against Alternaria spot of ginseng in the field were studied. The results showed that the strain could colonize in root, stem and leaf of ginseng and the colonization amount was positive correlated with inoculation concentration. Meanwhile, the strain could infect and then transfer in different tissues of ginseng The colonization amount of strain in roots and leaves of ginseng increased first and then decreased. However, the tendency of colonization amount of strain in stems was ascend at first and then descend slowly, and was more than that in roots and leaves along with time, which had a preference to specific tissue of its host. In field experiment, the endophytic bacteria NJ13 was proved to be effective in controlling Alternaria leaf spot of ginseng. The biocontrol efficiency of fermentation broth at the concentration of 0.76 x 10(8) cfu x mL(-1) reached 75.62%, which was close to the controlling level (73.06%) of 0.67 mg x L(-1) 50% cyprodinil WG.

  13. NY-ESO-1 (CTAG1B) expression in mesenchymal tumors.

    Science.gov (United States)

    Endo, Makoto; de Graaff, Marieke A; Ingram, Davis R; Lim, Simin; Lev, Dina C; Briaire-de Bruijn, Inge H; Somaiah, Neeta; Bovée, Judith V M G; Lazar, Alexander J; Nielsen, Torsten O

    2015-04-01

    New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies. We performed a large-scale immunohistochemical expression study of NY-ESO-1 using tissue microarrays of mesenchymal tumors from three institutions in an international collaboration. A total of 1132 intermediate and malignant and 175 benign mesenchymal lesions were enrolled in this study. Immunohistochemical staining was performed on tissue microarrays using a monoclonal antibody for NY-ESO-1. Among mesenchymal tumors, myxoid liposarcomas showed the highest positivity for NY-ESO-1 (88%), followed by synovial sarcomas (49%), myxofibrosarcomas (35%), and conventional chondrosarcomas (28%). Positivity of NY-ESO-1 in the remaining mesenchymal tumors was consistently low, and no immunoreactivity was observed in benign mesenchymal lesions. On the basis of these findings, nearly 90% of myxoid liposarcomas, as well as a significant proportion of synovial sarcomas, myxofibrosarcomas, and conventional chondrosarcomas are good candidates for immunotherapy targeting NY-ESO-1.

  14. NY-ESO-1 as a potential immunotherapeutic target in renal cell carcinoma.

    Science.gov (United States)

    Giesen, Eva; Jilaveanu, Lucia B; Parisi, Fabio; Kluger, Yuval; Camp, Robert L; Kluger, Harriet M

    2014-07-30

    Novel immune therapies targeting tumor specific antigens are being developed. Our purpose was to determine expression of the cancer testes antigen NY-ESO-1 in renal cell carcinoma (RCC), as NY-ESO-1 targeting approaches, particularly adoptive cell therapy, have not been evaluated in this disease. We employed tissue microarrays containing >300 unique RCC cases and adjacent benign renal tissue to determine NY-ESO-1 expression using a quantitative immunofluorescence method. In addition, we studied NY-ESO-1 expression in 35 matched primary and metastatic RCC specimens to assess concordance between different tumor sites. NY-ESO-1 was highly expressed in a subset of RCCs. Expression in primary RCC specimens was significantly higher than adjacent normal renal tissue (PESO-1 expression seen in clear cell RCC suggests that NY-ESO-1 targeting approaches should be studied in this disease. Expression is higher in metastatic sites, and discordance between primary and metastatic sites in some patients suggests that patient selection for these therapies should be based on expression in metastatic rather than nephrectomy specimens.

  15. Atmospheric Research in Ny-Aalesund. Proceedings from the Third NySMAC meeting. NILU, Kjeller, Norway, 9.-11. April 1997

    Energy Technology Data Exchange (ETDEWEB)

    Floeisand, Inga; Gernandt, Hartwig; Larsen, Elisabeth Stoltz; Stordal, Frode; Wada, Makoto

    1997-07-01

    Leading abstract. The Ny-Aalesund Seminar Series was conceived by NySMAC (Ny-Aalesund Science Managers Committee) as a means of bringing together research scientists involved in programmes running at Ny-Aalesund or having an interest in that area. The first Seminar was held in Potsdam in Germany in May 1995, and included talks which covered all current research disciplines; physical, atmospheric, earth and biological sciences. The Second Seminar was devoted to biological science and particularly to ecology. It was held in Cambridge in UK in February 1996. The subject of this Third Seminar, held in Kjeller in Norway in April 1997, is atmospheric research in Ny-Aalesund. Since the early 1980s a wide range of projects have been carried out on Svalbard and particularly at Ny-Aalesund. Prominent in this research have been several international research programmes with emphasis on topics of e.g. Arctic (Arctic Monitoring and Assessment Programme, AMAP), European (European Monitoring and Evaluation Programme, EMEP) or global (International Geosphere-Biosphere Programme, IGBP; World Climate Research Programme, WCRP) scales. Atmospheric observations have been carried out in Ny-Aalesund in Svalbard for several years. From the early 1980s, the long range transport of sulphur dioxide and sulphate aerosols leading to Arctic Haze, has been studied. As a consequence, EMEP included Ny-Aalesund in their network. In the following years the transport of ozone and chemical precursors were observed, and recently the naturally occurring phenomenon of rapid surface ozone loss has been given particular attention. From the late 1980s observations of the stratospheric ozone layer and related chemical species were undertaken, and the efforts have increased considerably since. Ny-Aalesund is now one of the primary Arctic sites of the NDSC (Network for Detection of Stratospheric Change) network. Important efforts have been undertaken to participate in the European campaigns as the European

  16. [Prokaryotic expression, purification and identification of NY-ESO-1/GST fusion protein in E.coli].

    Science.gov (United States)

    Tang, Lei; Song, Chao-jun; Sun, Yuan-jie; Li, Na; Wei, Yu-ying; Sun, Yi; Yang, Kun

    2012-10-01

    To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E.coli. NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-1 downstream tagged by GST to construct the expression plasmid pGEX-4T1-NY-ESO-1. The recombinant vector was transformed to BL21 (DE3) and NY-ESO-1/GST fusion protein was induced expression by IPTG. The protein was purified by urea elution and identified by SDS-PAGE and Western blotting. The NY-ESO-1 segment was successfully amplified and its sequence was identical with that published in GenBank. The BL21 (DE3) pLysS containing the pGEX-4T1-NY-ESO-1 expressed a M(r); 44 000 fusion protein under the induction of IPTG. The purity of the protein was 90%. Western blotting proved that NY-ESO-1/GST had a specific reaction with anti-GST mAb. The prokaryotic expression vector of NY-ESO-1 has been constructed and the fusion protein NY-ESO-1/GST of high purity is successfully expressed.

  17. Attracting Urban Minority Students to Geosciences through Exposure to Careers and Applied Aspects in Newark, NJ

    Science.gov (United States)

    Gates, A. E.; Kalczynski, M. J.

    2014-12-01

    A solid pipeline of URM students into the Geosciences has been established in Newark, NJ by introducing them to applied opportunities. Prior to an OEDG program designed to engage URM students, there were no students from or near Newark interested in pursuing geosciences at Rutgers-Newark or Essex Community College, the two local urban campuses. By infusing activities that showed the applied aspects of geoscience and opportunities for careers into regular high school lesson plans, a significant number of students became interested. These students were recruited into a 4-week modular summer institute that focused on energy, mining resources, environment and surface processes. About 90 students per year attended the institute which included 2 local field trips per week, presentations by industry professionals, activities that placed academic subjects into career perspective and a research project that directly affected the well-being of the students and their families. The most interested dozen of the 90 students were invited to participate in a high profile applied project that received significant media coverage, further enhancing their impression of the importance of geosciences. Previous graduates of the program were employed as assistants in subsequent programs to recycle the experience and enthusiasm. This had a positive effect on the persistence of the assistants who viewed themselves as role models to the younger students. The results are burgeoning numbers of URM geoscience majors at Rutgers, offering of geoscience for the first time in 30 years at Essex Community College as well as a new 2+2 geoscience track and several dual-credit courses at local high schools. An important aspect of this pathway or pipeline is that students must be able to clearly see the next step and their role in it. They are very tentative in this essentially pioneering pursuit. If they don't get a sense of a welcoming community and an ultimate career outcome, they quickly lose

  18. NOAA Digital Orthophotography and Ancillary Oblique Imagery Collection for the Coasts of Main/New Hampshire, Massachusetts/Rhode Island/Connecticut, and Hudson River/Long Island /NY/NJ

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Project: NOAA Digital Orthophotography and Ancillary Oblique Imagery Collection for the Coasts of Main/New Hampshire, Massachusetts/Rhode Island/Connecticut, and...

  19. North Atlantic Environmental Sensitivity Index (ESI) Massachusetts (2001), Delaware Bay (1996), New Hampshire (2004), Hudson River (2006), and Rhode Island, Connecticut, NY/NJ Metro Area (2001) maps and geographic information systems data (NODC Accession 0020555)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These ESI data were collected, mapped, and digitized to provide environmental data for oil spill planning and response. The Clean Water Act with amendments by the...

  20. Antibody responses against NY-ESO-1 and HER2 antigens in patients vaccinated with combinations of cholesteryl pullulan (CHP)-NY-ESO-1 and CHP-HER2 with OK-432.

    Science.gov (United States)

    Aoki, Masatoshi; Ueda, Shugo; Nishikawa, Hiroyoshi; Kitano, Shigehisa; Hirayama, Michiko; Ikeda, Hiroaki; Toyoda, Hideki; Tanaka, Kyosuke; Kanai, Michiyuki; Takabayashi, Arimichi; Imai, Hiroshi; Shiraishi, Taizo; Sato, Eiichi; Wada, Hisashi; Nakayama, Eiichi; Takei, Yoshiyuki; Katayama, Naoyuki; Shiku, Hiroshi; Kageyama, Shinichi

    2009-11-16

    Combination vaccines of the NY-ESO-1 protein complexed with cholesteryl pullulan (CHP), CHP-NY-ESO-1, and the truncated 146HER2 protein with CHP, CHP-HER2, were subcutaneously administered with the immuno-adjuvant OK-432 to eight esophageal cancer patients. Vaccination was well-tolerated. NY-ESO-1- and HER2-specific antibody responses were analyzed using the patients' sera and samples from previous single CHP-NY-ESO-1 or CHP-HER2 vaccine trial. The responses to NY-ESO-1 in the combination vaccine study were comparable to the single vaccine. For responses to HER2, there were fewer antibody responses in the combination vaccines. Although there were marked individual variations in the antibody responses to the NY-ESO-1 and HER2 antigens, the reaction patterns to these antigens were comparable within each patient. Antibodies to OK-432 were not augmented. Protein cancer vaccines targeting multiple antigens are feasible.

  1. 77 FR 5743 - Special Anchorage Areas; Port of New York, NY

    Science.gov (United States)

    2012-02-06

    ... intended to increase the safety of life and property in New York City and Atlantic Highlands, NJ, improve... Street Boat Basin. The second of these areas is approximately 2,630 yards by 160 yards, encompasses 89.90... on their vessels at night. Leaving their lights on overnight could result in dead batteries...

  2. NY-ESO-1 expression in meningioma suggests a rationale for new immunotherapeutic approaches.

    Science.gov (United States)

    Baia, Gilson S; Caballero, Otavia L; Ho, Janelle S Y; Zhao, Qi; Cohen, Tzeela; Binder, Zev A; Salmasi, Vafi; Gallia, Gary L; Quinones-Hinojosa, Alfredo; Olivi, Alessandro; Brem, Henry; Burger, Peter; Strausberg, Robert L; Simpson, Andrew J G; Eberhart, Charles G; Riggins, Gregory J

    2013-11-01

    Meningiomas are the most common primary intracranial tumors. Surgical resection remains the treatment of choice for these tumors. However, a significant number of tumors are not surgically accessible, recur, or become malignant, necessitating the repetition of surgery and sometimes radiation. Chemotherapy is rarely used and is generally not recognized as an effective treatment. Cancer/testis (CT) genes represent a unique class of genes, which are expressed by germ cells, normally silenced in somatic cells, but activated in various cancers. CT proteins can elicit spontaneous immune responses in patients with cancer and this feature makes them attractive targets for immunotherapy-based approaches. We analyzed mRNA expression of 37 testis-restricted CT genes in a discovery set of 18 meningiomas by reverse transcription PCR. The overall frequency of expression of CT genes ranged from 5.6% to 27.8%. The most frequently expressed was NY-ESO-1, in 5 patients (27.8%). We subsequently analyzed NY-ESO-1 protein expression in a larger set of meningiomas by immunohistochemistry and found expression in 108 of 110 cases. In some cases, NY-ESO-1 expression was diffused and homogenous, but in most instances it was heterogeneous. Importantly, NY-ESO-1 expression was positively correlated with higher grade and patients presenting with higher levels of NY-ESO-1 staining had significantly worse disease-free and overall survival. We have also shown that NY-ESO-1 expression may lead to humoral immune response in patients with meningioma. Considering the limited treatment options for patients with meningioma, the potential of NY-ESO-1-based immunotherapy should be explored. ©2013 AACR.

  3. Efficacy of systemic adoptive transfer immunotherapy targeting NY-ESO-1 for glioblastoma.

    Science.gov (United States)

    Everson, Richard G; Antonios, Joseph P; Lisiero, Dominique N; Soto, Horacio; Scharnweber, Rudi; Garrett, Matthew C; Yong, William H; Li, Ning; Li, Gang; Kruse, Carol A; Liau, Linda M; Prins, Robert M

    2016-03-01

    Immunotherapy is an ideal treatment modality to specifically target the diffusely infiltrative tumor cells of malignant gliomas while sparing the normal brain parenchyma. However, progress in the development of these therapies for glioblastoma has been slow due to the lack of immunogenic antigen targets that are expressed uniformly and selectively by gliomas. We utilized human glioblastoma cell cultures to induce expression of New York-esophageal squamous cell carcinoma (NY-ESO-1) following in vitro treatment with the demethylating agent decitabine. We then investigated the phenotype of lymphocytes specific for NY-ESO-1 using flow cytometry analysis and cytotoxicity against cells treated with decitabine using the xCelligence real-time cytotoxicity assay. Finally, we examined the in vivo application of this immune therapy using an intracranially implanted xenograft model for in situ T cell trafficking, survival, and tissue studies. Our studies showed that treatment of intracranial glioma-bearing mice with decitabine reliably and consistently induced the expression of an immunogenic tumor-rejection antigen, NY-ESO-1, specifically in glioma cells and not in normal brain tissue. The upregulation of NY-ESO-1 by intracranial gliomas was associated with the migration of adoptively transferred NY-ESO-1-specific lymphocytes along white matter tracts to these tumors in the brain. Similarly, NY-ESO-1-specific adoptive T cell therapy demonstrated antitumor activity after decitabine treatment and conferred a highly significant survival benefit to mice bearing established intracranial human glioma xenografts. Transfer of NY-ESO-1-specific T cells systemically was superior to intracranial administration and resulted in significantly extended and long-term survival of animals. These results reveal an innovative, clinically feasible strategy for the treatment of glioblastoma. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Neuro

  4. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination.

    Science.gov (United States)

    Kawada, Junji; Wada, Hisashi; Isobe, Midori; Gnjatic, Sacha; Nishikawa, Hiroyoshi; Jungbluth, Achim A; Okazaki, Nami; Uenaka, Akiko; Nakamura, Yurika; Fujiwara, Shinichi; Mizuno, Naoaki; Saika, Takashi; Ritter, Erika; Yamasaki, Makoto; Miyata, Hiroshi; Ritter, Gerd; Murphy, Roger; Venhaus, Ralph; Pan, Linda; Old, Lloyd J; Doki, Yuichiro; Nakayama, Eiichi

    2012-02-01

    NY-ESO-1 is a prototypic cancer/testis antigen. In a recent phase I clinical trial, we vaccinated 13 patients bearing NY-ESO-1-expressing tumors with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1) and showed efficient induction of NY-ESO-1 antibody, and CD4 and CD8 T cell responses using peripheral blood from the patients. In our study, we analyzed heteroclitic serological responses in those patients after vaccination. Serological response against 11 tumor antigens including MAGE-A1, MAGE-A3, MAGE-A4, CT7/MAGEC1, CT10/MAGEC2, CT45, CT46/HORMAD1, SOX2, SSX2, XAGE1B and p53 was examined by enzyme-linked immunosorbent assay (ELISA) using sera from ten vaccinated patients. Expression of tumor antigens was determined by reverse transcription-polymerase chain reaction or immunohistochemistry. Eight of nine patients who showed antibody responses against NY-ESO-1 also showed an antibody response against at least 1 of these 11 tumor antigens after vaccination. In one patient, seven tumor antigens were recognized. Specificity analysis of the antibody response by ELISA using control recombinant proteins and synthetic peptides and by Western blot showed that the response was not against His6-tag and/or bacterial products included in a preparation of CHP-NY-ESO-1 used for vaccination. Thus, heteroclitic serological responses appear to be indicative of the overall immune response against the tumor, and their analysis could be useful for immune monitoring in cancer vaccine. Copyright © 2011 UICC.

  5. CTLA-4 blockade enhances polyfunctional NY-ESO-1 specific T cell responses in metastatic melanoma patients with clinical benefit.

    Science.gov (United States)

    Yuan, Jianda; Gnjatic, Sacha; Li, Hao; Powel, Sarah; Gallardo, Humilidad F; Ritter, Erika; Ku, Geoffrey Y; Jungbluth, Achim A; Segal, Neil H; Rasalan, Teresa S; Manukian, Gregor; Xu, Yinyan; Roman, Ruth-Ann; Terzulli, Stephanie L; Heywood, Melanie; Pogoriler, Evelina; Ritter, Gerd; Old, Lloyd J; Allison, James P; Wolchok, Jedd D

    2008-12-23

    Blockade of inhibitory signals mediated by cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) has been shown to enhance T cell responses and induce durable clinical responses in patients with metastatic melanoma. The functional impact of anti-CTLA-4 therapy on human immune responses is still unclear. To explore this, we analyzed immune-related adverse events and immune responses in metastatic melanoma patients treated with ipilimumab, a fully human anti-CTLA-4 monoclonal antibody. Fifteen patients were selected on the basis of availability of suitable specimens for immunologic monitoring, and eight of these showed evidence of clinical benefit. Five of the eight patients with evidence of clinical benefit had NY-ESO-1 antibody, whereas none of seven clinical non-responders was seropositive for NY-ESO-1. All five NY-ESO-1 seropositive patients had clearly detectable CD4(+) and CD8(+) T cells against NY-ESO-1 following treatment with ipilimumab. One NY-ESO-1 seronegative clinical responder also had a NY-ESO-1 CD4(+) and CD8(+) T cell response, possibly related to prior vaccination with NY-ESO-1. Among five clinical non-responders analyzed, only one had a NY-ESO-1 CD4(+) T cell response and this patient did not have detectable anti-NY-ESO-1 antibody. Overall, NY-ESO-1-specific T cell responses increased in frequency and functionality during anti-CTLA-4 treatment, revealing a polyfunctional response pattern of IFN-gamma, MIP-1beta and TNF-alpha. We therefore suggest that CTLA-4 blockade enhanced NY-ESO-1 antigen-specific B cell and T cell immune responses in patients with durable objective clinical responses and stable disease. These data provide an immunologic rationale for the efficacy of anti-CTLA-4 therapy and call for immunotherapeutic designs that combine NY-ESO-1 vaccination with CTLA-4 blockade.

  6. The distribution of depleted uranium contamination in Colonie, NY, USA

    Energy Technology Data Exchange (ETDEWEB)

    Lloyd, N.S., E-mail: nsl3@alumni.leicester.ac.uk [Department of Geology, University of Leicester, University Road, Leicester, LE1 7RH (United Kingdom); Chenery, S.R.N. [British Geological Survey, Kingsley Dunham Centre, Keyworth, Nottingham, NG12 5GG (United Kingdom); Parrish, R.R. [Department of Geology, University of Leicester, University Road, Leicester, LE1 7RH (United Kingdom); NERC Isotope Geosciences Laboratory, Kingsley Dunham Centre, Keyworth, Nottingham, NG12 5GG (United Kingdom)

    2009-12-20

    Uranium oxide particles were dispersed into the environment from a factory in Colonie (NY, USA) by prevailing winds during the 1960s and '70s. Uranium concentrations and isotope ratios from bulk soil samples have been accurately measured using inductively coupled plasma quadrupole mass spectrometry (ICP-QMS) without the need for analyte separation chemistry. The natural range of uranium concentrations in the Colonie soils has been estimated as 0.7-2.1 {mu}g g{sup -1}, with a weighted geometric mean of 1.05 {mu}g g{sup -1}; the contaminated soil samples comprise uranium up to 500 {+-} 40 {mu}g g{sup -1}. A plot of {sup 236}U/{sup 238}U against {sup 235}U/{sup 238}U isotope ratios describes a mixing line between natural uranium and depleted uranium (DU) in bulk soil samples; scatter from this line can be accounted for by heterogeneity in the DU particulate. The end-member of DU compositions aggregated in these bulk samples comprises (2.05 {+-} 0.06) x 10{sup -3235}U/{sup 238}U, (3.2 {+-} 0.1) x 10{sup -5236}U/{sup 238}U, and (7.1 {+-} 0.3) x 10{sup -6234}U/{sup 238}U. The analytical method is sensitive to as little as 50 ng g{sup -1} DU mixed with the natural uranium occurring in these soils. The contamination footprint has been mapped northward from site, and at least one third of the uranium in a soil sample from the surface 5 cm, collected 5.1 km NNW of the site, is DU. The distribution of contamination within the surface soil horizon follows a trend of exponential decrease with depth, which can be approximated by a simple diffusion model. Bioturbation by earthworms can account for dispersal of contaminant from the soil surface, in the form of primary uranium oxide particulates, and uranyl species that are adsorbed to organic matter. Considering this distribution, the total mass of uranium contamination emitted from the factory is estimated to be c. 4.8 tonnes.

  7. Microstructure and phase transformation behavior of Mo-MoSi2 gradient material

    Institute of Scientific and Technical Information of China (English)

    Ying-Yi Zhang; Yun-Gang Li; Yong-Hong Qi; Xue-Feng Shi

    2015-01-01

    Mo-MoSi2 gradient coating on Mo substrate was prepared by siliconizing process,using the polysilicon as silicon atoms.The gradient layer was analyzed by the experimental results,theoretical analysis,and thermodynamic calculation.The silicon,molybdenum content of gradient coating shows a gradual change regulation.The reaction between silicon and molybdenum is most likely to generate Mo5Si3,then MoSi2,finally Mo3Si,but when the silicon content is excessive,the Mo5Si3 and Mo3Si will react with silicon and generate MoSi2.The gradient layer is mainly constituted by Si and MoSi2,only about 1/10 gradient layer is constituted by Mo5Si3 and Mo3Si,and the stable existences of Mo5Si3 and Mo3Si are mainly determined by the silicon content.Along the Mo substrate to the surface of the coating,the phase compo sition of gradient coating changes as follows:Mo → transition layer,Mo (main phase) + Mo3Si + Mo5Si3 →intermediate layer,and MoSi2 (main phase)+ Mo5Si3 + Si → surface layer MoSi2 (main phase)+ Si,and the experimental temperature has no effect on phase composition of gradient coating.

  8. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery

    OpenAIRE

    Xu, Heng; GU, NA; LIU, ZHAO-BO; Zheng, Min; Xiong, Fang; WANG, SI-YING; Li, Ning; Lu, Jun

    2011-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for th...

  9. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery

    OpenAIRE

    2011-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for th...

  10. Role Models and Mentors in Mid-Pipeline Retention of Geoscience Students, Newark, NJ

    Science.gov (United States)

    Gates, A. E.; Kalczynski, M. J.

    2012-12-01

    Undergraduate minority students retained enthusiasm for majoring in the geosciences by a combination of working with advanced minority mentors and role models as well as serving as role models for middle and high school students in Geoscience Education programs in Newark, NJ. An academic year program to interest 8-10th grade students from the Newark Public schools in the Geosciences employs minority undergraduate students from Rutgers University and Essex Community College as assistants. There is an academic year program (Geoexplorers) and a science festival (Dinosaur Day) at the Newark Museum that employs Rutgers University students and a summer program that employs Rutgers and Essex Community College students. All students are members of the Garden State LSAMP and receive any needed academic support from that program. The students receive mentoring from minority graduate students, project personnel and participating Newark Public School teachers, many of whom are from minority groups. The main factor in success and retention, however, is their role as authorities and role models for the K-12 students. The assistants are respected and consulted by the K-12 students for their knowledge and authority in the geosciences. This positive feedback shows them that they can be regarded as geoscientists and reinforces their self-image and enthusiasm. It further reinforces their knowledge of Geoscience concepts. It also binds the assistants together into a self-supporting community that even extends to the non-participating minority students in the Rutgers program. Although the drop-out rate among minority Geoscience majors was high (up to 100%) prior to the initiation of the program, it has dropped to 0% over the past 3 years with 2 participants now in PhD programs and 2 others completing MS degrees this year. Current students are seriously considering graduate education. Prior to this program, only one minority graduate from the program continued to graduate school in the

  11. Estimation of an NY-ESO-1 expressing HCC cell line by NY-ESO-1b specific CD8+T cells in vitro induced by HLA-A2 restricted NY-ESO-1b peptide%HLA-A2限制性NY-ESO-1b肽段诱导的CD8+ T细胞对稳定表达NY-ESO-1的HepG2肝癌细胞系的杀伤效应

    Institute of Scientific and Technical Information of China (English)

    乔欢; 钱晓萍; 张华刚; 田婵; 陈慰峰

    2005-01-01

    目的:分析体外诱导的NY-ESO-1特异性杀伤性T淋巴细胞对稳定表达NY-ESO-1蛋白的肝细胞性肝癌(hepatocellular carcinoma,HCC)细胞系的杀伤效应,为进一步分析NY-ESO-1蛋白作为疫苗用于治疗HCC的可能性提供实验支持.方法:从HLA-A2阳性的肝癌患者体内分离出外周血单个核细胞,用NY-ESO-1肽段体外诱导特异性杀伤性T淋巴细胞,并通过酶联免疫斑点法分析特异性杀伤性T淋巴细胞对稳定表达NY-ESO-1蛋白的HCC细胞系HepG2细胞的杀伤效应.结果:转染了NY-ESO-1的HepG2细胞(HLA-A2+)可有效地被NY-ESO-1157-165抗原肽诱导的特异性CD8+ T淋巴细胞所识别.效应细胞中大量GrB的释放表明其对稳定转染了NY-ESO-1基因的HepG2细胞有很强的杀伤作用.结论:HepG2细胞中表达的NY-ESO-1蛋白可被此肝癌细胞有效地加工处理,而且HLA-A2限制性的抗原肽NY-ESO-1157-165也可被有效地提呈到细胞表面,从而被效应T细胞所识别.

  12. Middle Permian palaeobiogeography study in East Kunlun, A'nyêmaqên and Bayan Har

    Institute of Scientific and Technical Information of China (English)

    WANG; Yongbiao; YANG; Hao

    2004-01-01

    Three regions can easily be identified in the study area according to the Middle Permian palaeobiogeographic distribution of biota, they are the southern slope of East Kunlun, A'nyêmaqên and Bayan Har. Biotic constitution and ecology in the southern slope of East Kunlun and Bayan Har are very similar. Both the diversity and abundance of organisms in these two areas are very high and reefs are widely developed. However, biotic diversity and abundance in A'nyêmaqên which is between the above two areas are obviously low. Differentiation of palaeobiogeographic distribution in these areas should be due to the baring of A'nyêmaqên ocean in the time of Middle Permian. Middle Permian radiolarian chert and thick abyssal red ooze are widely spread in A'nyêmaqên, implying that the A'nyêmaqên ocean had a great scale in size. Vast scale of deep ocean basin became an impassable gulf for some of the benthos, and as a result, only part of the organisms could have the chance to get to the isolated islands situated in ocean basin. Small living space and hard conditions in the islands further limited the abundance and diversity of biota. Tectonic background reflected by the geochemical study of basalt in the three areas is coupling well enough with the palaeobiogeographic division.

  13. Short-term Climate Characteristics at Ny-(A)lesund over the Arctic Tundra Area

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Based on the Germany Koldwey Station's 1994-2003 conventional observation hourly data, this paper conducts a statistical analysis on the short-term climate characteristics for an arctic tundra region (Ny-(A)lesund island) where our first arctic expedition station (Huanghe Station) was located. Affected by the North Atlantic warming current, this area has a humid temperate climate, and the air temperature at Ny-(A)lesund rose above 0 ℃ even during deep winter season during our research period. The wind speed in this area was low and appeared most at southeast direction. We find that the temperature at Ny-(A)lesund rose in the faster rate (0.68 ℃/10 a) than those at the whole Arctic area. Compared with the floating ices where our expedition conducted in the Arctic, Ny-(A)lesund was warmer and more humid and had lower wind speed. Comparison of the near surface air temperature derived by NCEP/NCAR reanalysis to the conventional measurements conducted at the Koldwey site in Ny-(A)lesund area shows a good agreement for winter season and a significant difference for summer season.

  14. MoS2 nanoflowers as high performance saturable absorbers for an all-fiber passively Q-switched erbium-doped fiber laser

    Science.gov (United States)

    Wei, Rongfei; Zhang, Hang; Tian, Xiangling; Qiao, Tian; Hu, Zhongliang; Chen, Zhi; He, Xin; Yu, Yongze; Qiu, Jianrong

    2016-03-01

    Strong saturable absorption was observed in MoS2 nanoflowers, which were synthesized by a facile solvothermal method. A MoS2 nanoflower-based saturable absorber with a high modulation depth of 51.8% and a large saturable intensity of 275.5 GW cm-2 was introduced to the application of passively Q-switched fiber laser generation. Stable passively Q-switched fiber laser pulses at 1.56 μm with a low threshold power of 16.10 mW, high signal-to-noise ratio of 52.5 dB and short pulse duration of 1.9 μs were obtained. More importantly, a high output power of 3.10 mW related to a large pulse energy of about 51.84 nJ can be attained at a very low pump power. The efficiency of the laser reaches 4.71%, which is larger than that of the prepared layered MoS2 and recently reported MoS2-based passively Q-switching operations. Such results imply that the MoS2 nanoflowers are an excellent candidate for a saturable absorber in passively Q-switched fiber lasers at a low pump intensity.Strong saturable absorption was observed in MoS2 nanoflowers, which were synthesized by a facile solvothermal method. A MoS2 nanoflower-based saturable absorber with a high modulation depth of 51.8% and a large saturable intensity of 275.5 GW cm-2 was introduced to the application of passively Q-switched fiber laser generation. Stable passively Q-switched fiber laser pulses at 1.56 μm with a low threshold power of 16.10 mW, high signal-to-noise ratio of 52.5 dB and short pulse duration of 1.9 μs were obtained. More importantly, a high output power of 3.10 mW related to a large pulse energy of about 51.84 nJ can be attained at a very low pump power. The efficiency of the laser reaches 4.71%, which is larger than that of the prepared layered MoS2 and recently reported MoS2-based passively Q-switching operations. Such results imply that the MoS2 nanoflowers are an excellent candidate for a saturable absorber in passively Q-switched fiber lasers at a low pump intensity. Electronic supplementary

  15. A phase I study of vaccination with NY-ESO-1f peptide mixed with Picibanil OK-432 and Montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Kakimi, Kazuhiro; Isobe, Midori; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Doki, Yuichiro; Nakajima, Jun; Seto, Yasuyuki; Yamatsuji, Tomoki; Naomoto, Yoshio; Shiraishi, Kenshiro; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Oka, Mikio; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi

    2011-12-15

    We conducted a phase I clinical trial of a cancer vaccine using a 20-mer NY-ESO-1f peptide (NY-ESO-1 91-110) that includes multiple epitopes recognized by antibodies, and CD4 and CD8 T cells. Ten patients were immunized with 600 μg of NY-ESO-1f peptide mixed with 0.2 KE Picibanil OK-432 and 1.25 ml Montanide ISA-51. Primary end points of the study were safety and immune response. Subcutaneous injection of the NY-ESO-1f peptide vaccine was well tolerated. Vaccine-related adverse events observed were fever (Grade 1), injection-site reaction (Grade 1 or 2) and induration (Grade 2). Vaccination with the NY-ESO-1f peptide resulted in an increase or induction of NY-ESO-1 antibody responses in nine of ten patients. The sera reacted with recombinant NY-ESO-1 whole protein as well as the NY-ESO-1f peptide. An increase in CD4 and CD8 T cell responses was observed in nine of ten patients. Vaccine-induced CD4 and CD8 T cells responded to NY-ESO-1 91-108 in all patients with various HLA types with a less frequent response to neighboring peptides. The findings indicate that the 20-mer NY-ESO-1f peptide includes multiple epitopes recognized by CD4 and CD8 T cells with distinct specificity. Of ten patients, two with lung cancer and one with esophageal cancer showed stable disease. Our study shows that the NY-ESO-1f peptide vaccine was well tolerated and elicited humoral, CD4 and CD8 T cell responses in immunized patients. Copyright © 2011 UICC.

  16. Generation of 8-nJ pulses from a dissipative-soliton fiber laser with a nonlinear optical loop mirror

    Science.gov (United States)

    Zhao, L. M.; Bartnik, A. C.; Tai, Q. Q.; Wise, F. W.

    2013-01-01

    Theoretical and experimental investigations of the behavior of normal-dispersion fiber lasers with nonlinear-optical loop mirrors are presented. The use of a loop mirror causes the laser to generate relatively long, flat-topped pulses. The pulse energy can be high, but the pulse duration is limited to greater than 300 fs. Experimentally, 8-nJ pulses that can be dechirped to 340 fs duration are obtained. The laser is a step toward an all-fiber, environmentally-stable design. PMID:23722797

  17. Widely-tunable, passively Q-switched erbium-doped fiber laser with few-layer MoS2 saturable absorber.

    Science.gov (United States)

    Huang, Yizhong; Luo, Zhengqian; Li, Yingyue; Zhong, Min; Xu, Bin; Che, Kaijun; Xu, Huiying; Cai, Zhiping; Peng, Jian; Weng, Jian

    2014-10-20

    We propose and demonstrate a MoS2-based passively Q-switched Er-doped fiber laser with a wide tuning range of 1519.6-1567.7 nm. The few-layer MoS2 nano-platelets are prepared by the liquid-phase exfoliation method, and are then made into polymer-composite film to construct the fiber-compatible MoS2 saturable absorber (SA). It is measured at 1560 nm wavelength, that such MoS2 SA has the modulation depth of ∼ 2% and the saturable optical intensity of ∼ 10 MW/cm(2). By further inserting the filmy MoS2-SA into an Er-doped fiber laser, stable Q-switching operation with a 48.1 nm continuous tuning from S- to C-waveband is successfully achieved. The shortest pulse duration and the maximum pulse energy are 3.3 μs and 160 nJ, respectively. The repetition rate and the pulse duration under different operation conditions have been also characterized. To the best of our knowledge, it is the first demonstration of MoS2 Q-switched, widely-tunable fiber laser.

  18. 芽孢杆菌R1共培养对嗜水气单胞菌NJ-1毒力基因表达的影响%Effects of coculture of Bacillus sp.R1 and Aeromonas hydrophila NJ-1 on the virulent factor gene expression of NJ-1

    Institute of Scientific and Technical Information of China (English)

    何夙旭; 王全民; 黄路; 周志刚

    2016-01-01

    为探讨芽孢杆菌潜在的淬灭靶点,本研究通过芽孢杆菌R1与嗜水气单胞菌NJ-1共培养,并对N J-1生物量、信号分子产生量、毒力因子基因和群体感应(QS)关键调控基因表达量进行检测.结果显示:①N J-1单独培养时,信号分子BHL和HHL的产量与NJ-1生长趋势一致,在27 h时达到峰值并启动LuxR表达,进而调控毒力因子金属蛋白酶、丝氨酸蛋白酶、肠毒素、气溶素和血溶素等表达,其在27~30 h时呈现峰值表达,通路QseB在27~30 h启动表达,48 h时达到峰值,而种间调控LuxS基因表达一直处于低水平状态;②与芽孢杆菌R1共培养时,仅痕量AHLs信号分子被检出,毒力因子相关基因表达量均显著下调;LuxR表达受抑制,LuxS在36h时表达量显著上调,未影响QseB正常表达;③AHLs与R1共培养可被显著降解.研究表明,芽孢杆菌R1抑制NJ-1毒力因子相关基因表达可能与其对NJ-1多QS系统进行调控相关联.

  19. Address Points, Address points, primarily derived from property tax records. Statewide coverage for NJ, but some addresses missing, particularly those where multiple addresses exist on a single parcel., Published in 2016, 1:2400 (1in=200ft) scale, NJ Office of GIS.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Address Points dataset, published at 1:2400 (1in=200ft) scale, was produced all or in part from Other information as of 2016. The source is NJ Office of GIS. It...

  20. 铜绿假单胞菌NY3所产表面活性剂对原油降解的影响%Effects of rhamnolipid on oil degradation by Pseudomonas aeruginosa strain NY3

    Institute of Scientific and Technical Information of China (English)

    常虹; 蒋欣; 聂麦茜; 葛碧洲; 刘超; 杨琴; 周立辉; 樊晓宇; 孙超; 韦绒绒

    2013-01-01

    铜绿假单胞菌NY3是从石油污染土壤中分离出的一株能快速代谢疏水性化合物的菌种.研究了该菌产表面活性剂及对原油降解的作用.实验表明,在敞开体系中,投加82 mg/L NY3菌产的鼠李糖脂,240 h能使NY3对原油的降解率提高50%.投加甘油使NY3产鼠李糖脂与降解原油同步进行,与投加鼠李糖脂对原油降解的促进作用相近.投加9%甘油使NY3在168 h对原油的降解率提高43%.NY3菌能同时降解原油中的直链烷烃及菲(Pr)和芘(Ph)等多环芳烃.在敞开体系中用少量甘油使产鼠李糖脂和降解原油同步进行,节约处理费用.研究结果为NY3菌株在露天石油污染环境修复中的应用奠定了基础.%Pseudomonas aeruginosa NY3 , being able to metabolize hydrophobic compounds, was isolated from petroleum-contaminated soil samples. Effects of the rhamnolipid and its production on crude oil degradation by NY3 strain were discussed. The results showed that the rhamnolipid produced by NY3 strain could enhance crude oil biodegradation. Comparing with the controls, oil removal rate within 240 h enhanced 50% with 82 mg/ L rhamnolipid. Adding glycerol in NY3 degrading systems, the degradation of crude oil and the rhamnolipid production took place simultaneously, the instantly produced rhamnolipid also accelerated oil metabolism. Adding 9‰ glycerol, the crude oil removal rate by NY3 improved about 43% within 168 h. Phenanthrene (Ph) and py-rene ( Pr) containing in the crude oil could also be removed. The cost of the remediation of contaminated water body by NY3 strain can be decreased by using small amount of glycerol, instead of using rhamnolipid directly. The above results lay a foundation for NY3 strain applying to oil-spill clean-up.

  1. Prokaryotic expression, purification and identification of NY-ESO-1/GST fusion protein in E.coli%NY-ESO-1/GST融合蛋白在大肠杆菌的表达、纯化及鉴定

    Institute of Scientific and Technical Information of China (English)

    汤蕾; 宋朝君; 孙元杰; 李娜; 魏玉英; 孙奕; 杨琨

    2012-01-01

    AIM: To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E. coli. METHODS: NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-l downstream tagged by GST to construct the expression plasmid pGEX4Tl-NY-ESO-l. The recombinant vector was transformed to BL21 ( DE3) and NY-ESO-1/ GST fusion protein was induced expression by IPTG. The protein was purified by urea elution and identified by SDS-PAGE and Western blotting. RESULTS: The NY-ESO-1 segment was successfully amplified and its sequence was identical with that published in GenBank. The BL21 (DE3) pLysS containing the pGEX-Tl-NY-ESO-1 expressed a M, 44 000 fusion protein under the induction of IPTG. The purity of the protein was 90%. Western blotting proved that NY-ESO-1/GST had a specific reaction with anti-GST mAb. CONCLUSION; The prokaryotic expression vector of NY-ESO-1 has been constructed and the fusion protein NY-ESO-1/GST of high purity is successfully expressed.%目的:构建肿瘤-睾丸抗原NY-ESO-1与GST融合基因的原核表达载体,在大肠杆菌中表达并对融合蛋白NY-ESO-1/GST进行纯化和初步鉴定.方法:设计针对NY-ESO-1的特异性引物,采用聚合酶链反应(PCR)从睾丸组织的cDNA文库扩增NY-ESO-1基因片段,经上下游引物所引入的EcoR I和Xho I双酶切后克隆人原核表达载体pGEX-4T1中GST标签的下游,构建重组表达载体pGEX-4T1-NY-ESO-1,并将该载体转化大肠杆菌BL21( DE3) pLysS中,IPTG诱导表达NYESO-1/GST融合蛋白,经不同浓度尿素洗脱纯化后,表达产物通过SDS-PAGE和Western blot法进行鉴定.结果:重组质粒经限制性内切酶EcoR I和Xho I双酶切鉴定和IPTG诱导表达NY-ESO-1/GST的SDS-PAGE分析表明,表达产物的相对分子质量(Mr)为44000,与理论值相符,并主要以包涵体形式存在,灰度扫描分析显示融合蛋白表

  2. Expression and enzymatic characterization of a cold-adapted β-agarase from Antarctic bacterium Pseudoalteromonas sp. NJ21

    Science.gov (United States)

    Li, Jiang; Sha, Yujie

    2015-03-01

    An agar-degrading bacterium, designated as Pseudoalteromonas sp. NJ21, was isolated from an Antarctic sediment sample. The agarase gene aga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase (rAga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30-40°C and 8.0, respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10°C, which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an β-agarase, producing neoagarobiose (NA2) as the final main product. Furthermore, this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.

  3. Vector similariton erbium-doped all-fiber laser generating sub-100-fs nJ pulses at 100 MHz.

    Science.gov (United States)

    Olivier, Michel; Piché, Michel

    2016-02-08

    Erbium-doped mode-locked fiber lasers with repetition rates comparable to those of solid-state lasers and generating nJ pulses are required for many applications. Our goal was to design a fiber laser that would meet such requirements, that could be built at relatively low cost and that would be reliable and robust. We thus developed a high-fundamental-repetition-rate erbium-doped all-fiber laser operating in the amplifier similariton regime. Experimental characterization shows that this laser, which is mode-locked by nonlinear polarization evolution, emits 76-fs pulses with an energy of 1.17 nJ at a repetition rate of 100 MHz. Numerical simulations support the interpretation of self-similar evolution of the pulse in the gain fiber. More specifically we introduce the concept of vector similariton in fiber lasers. The coupled x- and y- polarization components of such a pulse have a pulse profile with a linear chirp and their combined power profile evolves self-similarly when the nonlinear asymptotic regime is reached in the gain fiber.

  4. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Science.gov (United States)

    2010-07-01

    ... of Porter Avenue, Buffalo, N.Y. 110.84 Section 110.84 Navigation and Navigable Waters COAST GUARD... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning at...

  5. 77 FR 42510 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY; Correction

    Science.gov (United States)

    2012-07-19

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... College of Dentistry, New York, NY. The human remains were removed from Bronx County, NY. This notice is... was made by New York University College of Dentistry professional staff in consultation...

  6. 78 FR 62293 - Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY

    Science.gov (United States)

    2013-10-15

    ... CFR Part 165 RIN 1625-AA00 Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the navigable waters of Oyster Bay near Oyster Bay, NY for the...

  7. 78 FR 38310 - Kiwi Energy NY LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request...

    Science.gov (United States)

    2013-06-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Kiwi Energy NY LLC; Supplemental Notice That Initial Market-Based Rate... notice in the above-referenced proceeding, of Kiwi Energy NY LLC's application for market-based...

  8. Transfection of NY-ESO-1 specific TCR improves the specific cytotoxicity of human PBMC to NY-ESO-1 positive tumor cells%转染NY-ESO-1特异性TCR增强人PBMC对NY-ESO-1阳性肿瘤细胞的特异性细胞毒性

    Institute of Scientific and Technical Information of China (English)

    郭佳; 田洲; 顾娜; 徐珩; 闾军

    2013-01-01

    Objective To study whether NY-ESO-1-specific TCR gene transduced into human PBMC (Peripheral Blood Mononuclear Cell, PBMC) can improve its abilities of specifically recognizing and killing tumor cells. Methods pCDNA3. 1-ESO-TCR plasmid was electroporately transferred into PBMC separated from healthy people in vitro and confirmed by RT-PCR. The phenotype analysis after eletroporation was measured by flow cytometry method. The NY-ESO-1 specific peptide (pl57-165) was added into the culture of electroporated PBMC, the IFN-γ level secreted by electroporated PBMC was detected by ELISPOT assay. The specific cytotoxicity in vitro was detected by real time cell analysis (ACEA biosciences). Results The NY-ESO-1-specific TCR fragments in electroporated PBMC were detected by RT-PCR. The specific expression of NY-ESO-TCR in transferred PBMC was significantly higher than that in PBMC transfered with empty vector (P < 0. 05). The positive dots of IFN-γ secretion in transferred PBMC stimulated by peptide pl57-165 was significantly more than that in PBMC transfered with empty vector (P <0. 05); The specific cytotoxicity of transferred PBMC was obviously enhanced than PBMC transduced with empty vector. Conclusions The specific cytotoxicity of PBMC against NY-ESO-1 positive HepG2 cells is elevated by transduced PBMC with NY-ESO-1 specific TCR, which may stimulate more researches about adoptive immunotherapy of hepatocellular carcinoma.%目的 探讨体外转导NY-ESO-1特异性T细胞受体至人外周血淋巴细胞中,是否可增强转导后细胞体外特异性识别并杀伤肿瘤细胞的能力.方法 将NY-ESO-1特异性TCR质粒(pCDNA3.1-ESO-TCR)体外电转入新分离的正常人PBMC中,RT-PCR法鉴定转导是否成功;采用流式细胞仪分析转导后PBMC表型;用特异性NY-ESO-1b抗原肽(p157-165)刺激转导PBMC后,用ELISPOT法检测PBMC分泌IFN-γ的能力,用实时无标记动态细胞分析仪检测PBMC特异性细胞毒性作用.结果 电转后PBMC

  9. 95Mo NMR: hyperfine interactions in MoO3, MoS2, MoSe2 Mo3Se4, MoSi2 and Mo2C.

    Science.gov (United States)

    Bastow, T J

    1998-10-01

    Nuclear hyperfine interactions have been obtained by nuclear magnetic resonance (NMR) for 95Mo in a number of binary Mo compounds, both insulators and metals, which illustrate the interplay between nuclear quadrupole and chemical (Knight) shift terms. The insulating phases are characterised by nuclear spin lattice relaxation times greater than 100 s, demonstrating the ineffectiveness of indirect phonon Raman relaxation for these compounds.

  10. A potential role for immunotherapy in thyroid cancer by enhancing NY-ESO-1 cancer antigen expression.

    Science.gov (United States)

    Gunda, Viswanath; Frederick, Dennie T; Bernasconi, Maria J; Wargo, Jennifer A; Parangi, Sareh

    2014-08-01

    NY-ESO-1 is one of the most immunogenic members of the cancer/testis antigen family and its levels can be increased after exposure to demethylating and deacetylating agents. This cytoplasmic antigen can serve as a potent target for cancer immunotherapy and yet has not been well studied in differentiated thyroid cancer cells. We studied the baseline expression of NY-ESO-1 messenger RNA and protein before and after exposure to 5-aza-2'-deoxycytidine (DAC) (72 hours) in a panel of thyroid cancer cell lines using quantitative polymerase chain reaction and Western blot. HLA-A2+, NY-ESO-1+ thyroid cell lines were then co-cultured with peripheral blood lymphocytes transduced with NY-ESO-1 specific T-cell receptor (TCR) and assayed for interferon-gamma and Granzyme-B release in the medium. SCID mice injected orthotopically with BCPAP cells were treated with DAC to evaluate for NY-ESO-1 gene expression in vivo. None of the thyroid cancer cell lines showed baseline expression of NY-ESO-1. Three cell lines, BCPAP, TPC-1, and 8505c, showed an increase in NY-ESO-1 gene expression with DAC treatment and were found to be HLA-A2 positive. DAC-treated target BCPAP and TPC-1 tumor cells with up-regulated NY-ESO-1 levels were able to mount an appropriate interferon-gamma and Granzyme-B response upon co-culture with the NY-ESO-1-TCR-transduced peripheral blood lymphocytes. In vivo DAC treatment was able to increase NY-ESO-1 expression in an orthotopic mouse model with BCPAP cells. Our data suggest that many differentiated thyroid cancer cells can be pressed to express immune antigens, which can then be utilized in TCR-based immunotherapeutic interventions.

  11. 76 FR 43259 - Foreign-Trade Zone 109-Watertown, NY, Application for Manufacturing Authority, North American...

    Science.gov (United States)

    2011-07-20

    ...) with textile backing material for the U.S. market and export. The manufacturing process under FTZ..., North American Tapes, LLC, (Textile Athletic Tape), Watertown, NY A request has been submitted to the... textile fabrics (duty rates: 10.5, 14.9%). The facility can produce up to 12.5 million square meters of...

  12. 78 FR 51061 - Special Anchorage Areas; Port of New York, NY

    Science.gov (United States)

    2013-08-20

    ... convenient access to public transportation, onshore shopping and amenities which facilitate interstate and... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 110 RIN 1625-AA01 Special Anchorage Areas; Port of New York, NY AGENCY...

  13. Modelling dune erosion, overwash and breaching at Fire Island (NY) during hurricane Sandy

    NARCIS (Netherlands)

    De Vet, P.L.M.; McCall, R.T.; Den Bieman, J.P.; Stive, M.J.F.; Van Ormondt, M.

    2015-01-01

    In 2012, Hurricane Sandy caused a breach at Fire Island (NY, USA), near Pelican Island. This paper aims at modelling dune erosion, overwash and breaching processes that occured during the hurricane event at this stretch of coast with the numerical model XBeach. By using the default settings, the ero

  14. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Randall Bay, Freeport, Long Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND....Y. (a) The anchorage grounds. Southward of a line 312 feet south of and parallel to the south...

  15. 78 FR 23845 - Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY

    Science.gov (United States)

    2013-04-23

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY AGENCY... the Smith Point Bridge, mile 6.1, across Narrow Bay, between Smith Point and Fire Island, New York. The deviation is necessary to facilitate the Smith Point Triathlon. This deviation allows the...

  16. 76 FR 60733 - Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY

    Science.gov (United States)

    2011-09-30

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY AGENCY... the Smith Point Bridge, 6.1, across Narrow Bay, between Smith Point and Fire Island, New York. The.... SUPPLEMENTARY INFORMATION: The Smith Point Bridge, across Narrow Bay, mile 6.1, between Smith Point and...

  17. Structural and magnetic properties of nanocomposite iron-containing SiCxNy films

    Science.gov (United States)

    Pushkarev, R. V.; Fainer, N. I.; Maurya, K. K.

    2017-02-01

    New ferromagnetic films with composition SiCxNyFez were synthesized using chemical vapor deposition technique. Films were deposited using ferrocene, 1,1,1,3,3,3-hexamethyldisilazane (HMDS) and hydrogen gaseous mixture. Chemical and phase composition of the films were studied by FTIR, Raman spectroscopy and X-ray diffraction with grazing incidence (GI-XRD). FTIR spectra analysis confirmed the existence of Si-C and Si-N bonds. Graphite inclusions and amorphous carbon were determined by Raman spectra analysis. The surface of the SiCxNyFez films studied by SEM is covered by nanocrystallites of iron oxide Fe3O4 phase. The main purpose of GI-XRD analysis is to describe the layered structure of the films in detail. It was shown by GI-XRD study, that phase composition of the SiCxNyFez films varies from iron oxide Fe3O4 to iron silicide Fe3Si and silicon carbide SiC with the deposition temperature growing. It was established, that SiCxNyFez films are perspective for application in the spintronic field.

  18. 76 FR 77175 - New York Fun Factory Fireworks Display, Western Long Island Sound; Mamaroneck, NY

    Science.gov (United States)

    2011-12-12

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 New York Fun Factory Fireworks Display, Western Long... Island Sound in the vicinity of Mamaroneck, NY in support of the New York Fun Factory Fireworks display... Guard to define regulatory safety zones. On May 10, 2012 New York Fun Factory Events is sponsoring...

  19. Den digitale patientmappe - et bud på en ny form for sundhedskommunikation i fysioterapeutisk praksis

    DEFF Research Database (Denmark)

    Duus, Lene

    2012-01-01

    Præsentation af et udviklingsprojekt, som jeg har deltaget i, hvor en ny form for sundhedskommunikation gennem en netbaseret digital patientmappe i fysioterapeutisk praksis har været afprøvet. Det overordnede mål for projektet var at bedre kommunikation, dokumentation og compliance for privatprak...

  20. 77 FR 74866 - Notice of Intent To Repatriate Cultural Items: New York State Museum, Albany, NY

    Science.gov (United States)

    2012-12-18

    ... belts. Seven of the wampum belts are on loan to the Seneca National Museum in Salamanca, NY, and one... Jemison on the Cattaraugus Reservation in New York. Mary Jemison was adopted by the Seneca as a child and chose to live her life as a Seneca. At the time of collection, the Five Nations Alliance Belt...

  1. 77 FR 30312 - Montezuma National Wildlife Refuge, Cayuga, Seneca, and Wayne Counties, NY; Draft Comprehensive...

    Science.gov (United States)

    2012-05-22

    ... notice in the Federal Register (75 FR 25286; May 7, 2010). Montezuma NWR was established in 1938 to... Fish and Wildlife Service Montezuma National Wildlife Refuge, Cayuga, Seneca, and Wayne Counties, NY... assessment (draft CCP/EA) for Montezuma National Wildlife Refuge (NWR), located in Cayuga, Seneca, and...

  2. 76 FR 3516 - Drawbridge Operation Regulations; Harlem River, New York City, NY

    Science.gov (United States)

    2011-01-20

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Harlem River, New York City, NY... of the 103rd Street (Wards Island) Bridge at mile 0.0, across the Harlem River, New York City, New... vessels, and commercial vessels of various sizes. The owner of the bridge, New York City Department of...

  3. 78 FR 35756 - Drawbridge Operation Regulations; Harlem River, New York City, NY

    Science.gov (United States)

    2013-06-14

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Harlem River, New York City, NY... of the Willis Avenue Bridge across the Harlem River, mile 1.5, at the Bronx, New York. The deviation... existing drawbridge operating regulations are found at 33 CFR 117.789(b)(2). The bridge owner, New York...

  4. 78 FR 23846 - Drawbridge Operation Regulations; Newtown Creek, New York City, NY

    Science.gov (United States)

    2013-04-23

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Newtown Creek, New York City, NY... of the Greenpoint Avenue Bridge across Newtown Creek, mile 1.3, at New York City, New York. The... are found at 33 CFR 117.801(g). The bridge owner, New York City Department of Transportation...

  5. 75 FR 36110 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-24

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... Dentistry, New York, NY. The human remains were removed from Broward and Levy Counties, FL, and an unknown... assessment of the human remains was made by the New York University College of Dentistry professional...

  6. 75 FR 33327 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-11

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... Dentistry, New York, NY. The human remains were removed from the Allred Bluff and Salts Bluff Rockshelters... assessment of the human remains was made by the New York University College of Dentistry professional...

  7. 75 FR 33329 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-11

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... Dentistry, New York, NY. The human remains were removed from Lovelock Cave, Churchill County, NV. This... remains was made by the New York University College of Dentistry professional staff in consultation...

  8. 78 FR 36301 - CSX Transportation, Inc.-Discontinuance of Service Exemption-in Oswego County, NY

    Science.gov (United States)

    2013-06-17

    ... 0.85-mile rail line on CSXT's Northern Region, Albany Division, Fulton Subdivision, between milepost QMF 37.10 at the connection with CSXT's main line and milepost QMF 37.95 at the end of the track, in Oswego County, NY (the Line). The Line traverses United States Postal Service Zip Code 13126,...

  9. 78 FR 74009 - Safety Zone; Nike Fireworks, Upper New York Bay, Ellis Island, NY

    Science.gov (United States)

    2013-12-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 Safety Zone; Nike Fireworks, Upper New York Bay, Ellis Island, NY..., 2011 (76 FR 69614). ] Table 1 1. Nike Fireworks Ellis Launch site: A barge located Island Safety...

  10. 78 FR 71546 - Safety Zone; Belt Parkway Bridge Construction, Gerritsen Inlet, Brooklyn, NY

    Science.gov (United States)

    2013-11-29

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Belt Parkway Bridge Construction, Gerritsen Inlet, Brooklyn, NY AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The Coast... Belt Parkway Bridge. This proposed rule would allow the Coast Guard to prohibit all vessel traffic...

  11. 76 FR 4529 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY

    Science.gov (United States)

    2011-01-26

    ... 02'14'' W (NAD 83), approximately 70 yards southeast of the Verrazano Bridge Brooklyn tower. All... Bridge, Brooklyn, NY. This safety zone will not have a significant economic impact on a substantial...), approximately 70-yards southeast of the Verrazano Bridge Brooklyn tower. (b) Effective period. This regulation...

  12. 76 FR 34865 - Safety Zone; Rochester Harbor Festival, Genesee River, Rochester, NY

    Science.gov (United States)

    2011-06-15

    ... impracticable and contrary to the public interest. Background and Purpose The Rochester Harbor Festival is an... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Rochester Harbor Festival, Genesee River... establishing a temporary safety zone on the Genesee River, Rochester, NY for the Rochester Harbor Festival...

  13. 77 FR 52058 - Notice of Inventory Completion: American Museum of Natural History, New York, NY; Correction

    Science.gov (United States)

    2012-08-28

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY... completion of an inventory of human remains in the possession of the American Museum of Natural History, New... following sentence: Officials of the American Museum of Natural History have determined that, pursuant to...

  14. 76 FR 43712 - Notice of Inventory Completion: American Museum of Natural History, New York, NY

    Science.gov (United States)

    2011-07-21

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The American Museum of Natural History... human remains may contact the American Museum of Natural History. Repatriation of the human remains...

  15. NY-ESO-1 expression in DCIS: A new predictor of good prognosis.

    Science.gov (United States)

    Coombes, R Charles; Caballero, Otavia L; Shousha, Sami; Ghaem-Maghami, Sadaf; Woodley-Barker, Laura; Wilhelm-Benartzi, Charlotte S; Neville, A Munro

    2017-03-01

    At present, it is difficult to predict which patients with ductal carcinoma-in-situ (DCIS) will subsequently develop frank invasive breast cancer (IDC). A recent survey by our group has shown that NY-ESO-1 and MAGEA are both expressed in DCIS. This study was aimed at determining whether expression of these antigens was related to the later development of IDC. 14 of 42 (33%) of patients developed invasive breast cancer during the follow up period. Only one of those DCIS cases that relapsed was positive for NYESO-1 at diagnosis. In contrast, DCIS samples of 15 of the 28 (54%) of those patients who remained disease-free expressed NY-ESO-1. (Permutation chi square p=0.0033). We identified 42 patients with DCIS, and followed them up for more than 10 years. NY-ESO-1 and MAGEA were demonstrated by immunostaining as were CD8+ infiltrates on all sections together with the conventional markers, ER, PR, and HER2. Expression of NY-ESO-1 may predict those patients who will not subsequently develop invasive breast cancer and could therefore potentially be helpful in defining prognosis in patients with DCIS.

  16. 77 FR 46349 - Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY

    Science.gov (United States)

    2012-08-03

    ... significant environmental impact from this proposed rule. List of Subjects in 33 CFR Part 165 Harbors, Marine... Bay'' restaurant in Patchogue, NY. This rule will be effective from 8:30 p.m. on November 3, 2012... Broadcast Notice to Mariners. 2. Impact on Small Entities Under the Regulatory Flexibility Act (5 U.S.C....

  17. 75 FR 13454 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-03-22

    ... Federal Register (73 FR 3316). Public Meeting We do not plan to hold a public meeting. But you may submit...-2009-0520) in the Federal Register (74 FR 51243). No comments or requests for meetings were received... Championships, Huntington Bay, NY AGENCY: Coast Guard, DHS. ACTION: Supplemental Notice of proposed...

  18. NY Serpentis: SU UMa-type nova in the period gap with diversity of normal outbursts

    Science.gov (United States)

    Pavlenko, Elena P.; Kato, Taichi; Antonyuk, Oksana I.; Ohshima, Tomohito; Hambsch, Franz-Josef; Antonyuk, Kirill A.; Sosnovskij, Aleksei A.; Baklanov, Alex V.; Shugarov, Sergey Yu.; Pit, Nikolaj V.; Nakata, Chikako; Masi, Gianluca; Nakajima, Kazuhiro; Maehara, Hiroyuki; Dubovsky, Pavol A.; Kudzej, Igor; Andreev, Maksim V.; Kuznyetsova, Yuliana G.; Vasiliskov, Kirill A.

    2014-12-01

    We present a photometric study of NY Ser, an in-the-gap SU UMa-type nova, in 2002 and 2013. We determined the duration of its superoutburst and the mean period of its superhump are 18 d and 0.10458 d, respectively. We detected in 2013 that NY Ser showed two distinct states separated by a superoutburst. A state of rather infrequent normal outbursts lasted at least 44 d before the superoutburst, and a state of frequent outbursts started immediately after the superoutburst and lasted at least 34 d. Unlike a typical SU UMa star with a bimodal distribution of outburst duration, NY Ser displayed a diversity of normal outbursts. In the state of infrequent outbursts, we detected a wide ˜ 12 d outburst accompanied by 0.098 d orbital modulation but without superhumps ever established in NY Ser. We classified this as a "wide normal outburst." The orbital period was dominant both in quiescence and during normal outbursts in this state. In the state of the most frequent normal outbursts, the 0.10465 d positive superhump period was dominant and coexisted with the orbital modulation. In 2002 we detected the normal outburst of "intermediate" 5-6 d duration that was also accompanied by orbital modulations.

  19. 75 FR 15739 - International Business Machines Corporation: Armonk, NY; Notice of Termination of Investigation

    Science.gov (United States)

    2010-03-30

    ... Employment and Training Administration International Business Machines Corporation: Armonk, NY; Notice of... was initiated in response to a petition filed on May 21, 2009 on behalf of workers of International Business Machines Corporation, Armonk, New York. The petitioning group of workers is covered by...

  20. Nematodes from rocky grassland in Börzsöny Mountains, Hungary

    OpenAIRE

    Kiss, M

    2011-01-01

    This paper reports on investigations carried out on soil inhabiting nematodes living in andesitic rock regions ofBörzsöny Mountains. Seventeen species are listed. Heterodorus arcuatus (Thorne, 1939), new to the fauna of Hungary, isdescribed and illustrated in detail.

  1. Nematodes from rocky grassland in Börzsöny Mountains, Hungary

    Directory of Open Access Journals (Sweden)

    Kiss, M.

    2011-06-01

    Full Text Available This paper reports on investigations carried out on soil inhabiting nematodes living in andesitic rock regions ofBörzsöny Mountains. Seventeen species are listed. Heterodorus arcuatus (Thorne, 1939, new to the fauna of Hungary, isdescribed and illustrated in detail.

  2. 77 FR 41271 - Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson River, NY

    Science.gov (United States)

    2012-07-13

    ... Acronyms DHS Department of Homeland Security FR Federal Register CFR Code of Federal Regulations NPRM... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson... Newburgh, NY for the annual Newburgh Beacon Swim event. This temporary safety zone is necessary to...

  3. 77 FR 834 - Noise Exposure Map Update for Albany International Airport, Albany, NY

    Science.gov (United States)

    2012-01-06

    ... Federal Aviation Administration Noise Exposure Map Update for Albany International Airport, Albany, NY... Administration (FAA) announces its determination that the updated noise exposure maps submitted by the Albany.... 47501 et. seq (Aviation Safety and Noise Abatement Act) and 14 CFR part 150 are in compliance...

  4. 77 FR 51614 - CSX Transportation, Inc.-Abandonment Exemption-in Niagara County, NY

    Science.gov (United States)

    2012-08-24

    ... Abandonments to abandon a 0.1-mile rail line on its Northern Region, Albany Division, Niagara Subdivision, between milepost QDN 28.0 near North Avenue to the end of the track at milepost QDN 28.1, in Niagara Falls... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Niagara County, NY...

  5. 77 FR 41048 - Safety Zone; Hudson Valley Triathlon, Ulster Landing, Hudson River, NY

    Science.gov (United States)

    2012-07-12

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Hudson Valley Triathlon, Ulster Landing... Landing, NY for the 16th Annual Hudson Valley Triathlon swim event. This temporary safety zone is.... Regulatory History and Information The Hudson Valley Triathlon swim is an annual recurring event that has...

  6. 75 FR 11937 - Eagle Sportswear, Inc.; New York, NY; Notice of Termination of Investigation

    Science.gov (United States)

    2010-03-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Eagle Sportswear, Inc.; New York, NY; Notice of Termination of... response to a petition filed on December 4, 2009 by a company official on behalf of workers of...

  7. Influence of residual stress on the phase equilibrium of Ti(CxNy) thin films

    Institute of Scientific and Technical Information of China (English)

    Ying Jin; Changrong Li; Xiaoyan Ma; Zhenmin Du; Weijing Zhang; J.-C. Tedenac

    2003-01-01

    In order to provide a theoretic basis for the research of Ti(CxNy) thin films, the thermodynamic database of Ti-C-N ternary system is established and the phase diagram sections are calculated. In addition to the assessed thermodynamic properties of Ti-C-N system, the influence of the residual strain energy of Ti(CxNy) thin films on the phase equilibria is analyzed. The classical formula for calculating the elastic strain energy is expressed into a Redlich-Kister form in order to perform the thermodynamic and equilibrium calculations using the Thermo-Calc software. Isothermal sections at 900 and 1100 K are calculated with this database and compared with those calculated without considering the residual stress. As a result, with the addition of strain energy δ-fcc Ti(CxNy) phase area shrinks. It is therefore concluded that with the influence of the residual stress in Ti(CxNy) thin solid film, the precipitation of pure δ film requires more precise control of composition.

  8. Modelling dune erosion, overwash and breaching at Fire Island (NY) during hurricane Sandy

    NARCIS (Netherlands)

    De Vet, P.L.M.; McCall, R.T.; Den Bieman, J.P.; Stive, M.J.F.; Van Ormondt, M.

    2015-01-01

    In 2012, Hurricane Sandy caused a breach at Fire Island (NY, USA), near Pelican Island. This paper aims at modelling dune erosion, overwash and breaching processes that occured during the hurricane event at this stretch of coast with the numerical model XBeach. By using the default settings, the

  9. 77 FR 38492 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

    Science.gov (United States)

    2012-06-28

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY... temporary safety zone on Lake Ontario, Olcott, New York. This safety zone is intended to restrict vessels from a portion of Lake Ontario during the Olcott fireworks on July 3, 2012. The safety zone...

  10. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Katy Milne

    Full Text Available BACKGROUND: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC. A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens. METHODOLOGY AND PRINCIPAL FINDINGS: We obtained matched serum and tumor tissue from 35 patients with high-grade serous ovarian cancer. Serum samples were assessed by ELISA for autoantibodies to the common tumor antigen NY-ESO-1. Tumor tissue was examined by immunohistochemistry for expression of NY-ESO-1, various T cell markers (CD3, CD4, CD8, CD25, FoxP3, TIA-1 and Granzyme B and other immunological markers (CD20, MHC class I and MHC class II. Lymphocytic infiltrates varied widely among tumors and included cells positive for CD3, CD8, TIA-1, CD25, FoxP3 and CD4. Twenty-six percent (9/35 of patients demonstrated serum IgG autoantibodies to NY-ESO-1, which were positively correlated with expression of NY-ESO-1 antigen by tumor cells (r = 0.57, p = 0.0004. Autoantibodies to NY-ESO-1 were associated with increased tumor-infiltrating CD8+, CD4+ and FoxP3+ cells. In an individual HLA-A2+ patient with autoantibodies to NY-ESO-1, CD8+ T cells isolated from solid tumor and ascites were reactive to NY-ESO-1 by IFN-gamma ELISPOT and MHC class I pentamer staining. CONCLUSION AND SIGNIFICANCE: We demonstrate that tumor-specific autoantibodies and tumor-infiltrating T cells are correlated in human cancer and can be directed against the same target antigen. This implies that autoantibodies may collaborate with tumor-infiltrating T cells to influence clinical outcomes in EOC. Furthermore, serological screening methods may prove useful for identifying clinically relevant

  11. Mo-Si alloy development

    Energy Technology Data Exchange (ETDEWEB)

    Liu, C.T.; Heatherly, L.; Wright, J.L. [Oak Ridge National Lab., TN (United States)

    1996-06-01

    The objective of this task is to develop new-generation corrosion-resistant Mo-Si intermetallic alloys as hot components in advanced fossil energy conversion and combustion systems. The initial effort is devoted to Mo{sub 5}-Si{sub 3}-base (MSB) alloys containing boron additions. Three MSB alloys based on Mo-10.5Si-1.1B (wt %), weighing 1500 g were prepared by hot pressing of elemental and alloy powders at temperatures to 1600{degrees}C in vacuum. Microporosities and glassy-phase (probably silicate phases) formations are identified as the major concerns for preparation of MSB alloys by powder metallurgy. Suggestions are made to alleviate the problems of material processing.

  12. Dipole strength distribution below the giant dipole resonance in {sup 92}Mo, {sup 98}Mo and {sup 100}Mo

    Energy Technology Data Exchange (ETDEWEB)

    Rusev, G.Y.

    2006-07-01

    Investigations of the dipole-strength distributions in {sup 92}Mo, {sup 98}Mo and {sup 100}Mo were carried out by means of the method of nuclear resonance fluorescence. The low-lying excitations in the nuclides {sup 92}Mo, {sup 98}Mo and {sup 100}Mo have been studied in photon-scattering experiments at an electron energy of 6 MeV at the ELBE accelerator and at electron energies from 3.2 to 3.8 MeV at the Dynamitron accelerator. Five levels were observed in {sup 92}Mo. Five levels in {sup 98}Mo and 14 in {sup 100}Mo were identified for the first time in the energy range from 2 to 4 MeV. Dipole-strength distributions up to the neutron-separation energies in the nuclides {sup 92}Mo, {sup 98}Mo and {sup 100}Mo have been investigated at the ELBE accelerator. Because of the possible observation of transitions in the neighboring nuclei produced via ({gamma},n) reaction, additional measurements at electron energies of 8.4 and 7.8 MeV, below the neutron-separation energy, were performed on {sup 98}Mo and {sup 100}Mo, respectively. The number of transitions assigned to {sup 92}Mo, {sup 98}Mo and {sup 100}Mo is 340, 485 and 499, respectively, the main part of them being dipole transitions. Statistical properties of the observed transitions are obtained. The continuum contains the ground-state transitions as well as the branching transitions to the low-lying levels and the subsequent deexcitations of these levels. (orig.)

  13. The tubulin-bound conformation of paclitaxel: T-taxol vs "PTX-NY".

    Science.gov (United States)

    Yang, Yutao; Alcaraz, Ana A; Snyder, James P

    2009-03-27

    Nearly 35 years after its discovery and 11 years after FDA approval of paclitaxel (PTX) as a breakthrough anticancer drug, the 3-D structure of the agent bound to its beta-tubulin target was proposed to be T-Taxol. The latter bioactive form has recently been challenged by the Ojima group with a structure, "PTX-NY" ("REDOR Taxol"), in which the C-13 side chain is proposed to adopt a different conformation and an alternative hydrogen-bonding pattern in the tubulin binding site. Previously, the two conformers were compared to show that only T-Taxol fits the PTX-derived electron crystallographic density. That work has been extended by molecular mechanics and quantum chemical methods to reveal that the PTX-NY conformation is relatively less stable, on average, by 10-11 kcal/mol. In agreement with NMR studies, an 11 ns molecular dynamics treatment for PTX in an explicit water pool locates T-Taxol along the trajectory, but not PTX-NY. Docking of various PTX conformers into the electron crystallographic binding site of tubulin demonstrates that PTX-NY cannot be accommodated unless the pocket is reorganized in violation of the experimental constraints. Finally, analysis of the structures of T-Taxol and PTX-NY for their capacity to predict the existence of superpotent PTX analogues discloses that only the former forecasts such analogues, as now established by the T-Taxol-inspired synthesis of bridged taxanes. In sum, all empirical criteria support T-Taxol as the bound conformation of PTX on beta-tubulin in microtubules.

  14. Level structure of 89Mo

    Science.gov (United States)

    García-Bermúdez, G.; Cardona, M. A.; Ribas, R. V.; Filevich, A.; Achterberg, E.; Szybisz, L.

    1993-10-01

    The level structure of 89Mo has been studied with the 60Ni(32S,2pn) reaction at 110 MeV beam energy. Mainly two bands of γ rays depopulating states of probable spin-parity values ranging up to (27/2)+ were determined from the excitation function, neutron and γ-γ coincidences, and γ-ray angular distributions. The high-spin states of 89Mo show a scheme very similar to that of the 87Zr isotone. The interpretation of these states in terms of the three neutron-hole configuration (νg9/2)-3 and core-excited states is discussed.

  15. NY-BR-1 protein expression in breast carcinoma: a mammary gland differentiation antigen as target for cancer immunotherapy.

    Science.gov (United States)

    Theurillat, Jean-Philippe; Zürrer-Härdi, Ursina; Varga, Zsuzsanna; Storz, Martina; Probst-Hensch, Nicole M; Seifert, Burkhardt; Fehr, Mathias K; Fink, Daniel; Ferrone, Soldano; Pestalozzi, Bernhard; Jungbluth, Achim A; Chen, Yao-Tseng; Jäger, Dirk; Knuth, Alexander; Moch, Holger

    2007-11-01

    NY-BR-1 is a recently identified differentiation antigen of the mammary gland. To use NY-BR-1 for T-cell-based immunotherapy, analysis of its co-expression with HLA class I antigens is required. In the present tissue microarray study, primary breast cancers (n = 1,444), recurrences (n = 88), lymph node (n = 525) and distant metastases (n = 91) were studied for NY-BR-1 expression using a novel monoclonal antibody. NY-BR-1 expression was compared with prognosis, estrogen receptor, HER2-status, EGFR and HLA class I antigen expression. NY-BR-1 was more frequently expressed in grade 1 (82%) than in grade 2 (69%) and grade 3 (46%) carcinomas (P < 0.0001). Moreover, NY-BR-1 expression correlated directly with estrogen receptor expression (P < 0.0001) and inversely correlated with HER2-status and EGFR expression (P < 0.0001 for both). Considering high expression level of co-expression, 198/1,321 (15%) primary breast carcinomas and 4/65 (6%) distant metastases expressed NY-BR-1 and HLA class I, suggesting that active immunotherapy can be applied to about 10% of breast cancer patients. Survival analysis showed an association of NY-BR-1 expression with better patient outcome (P = 0.015). No difference between NY-BR-1 expression of primary tumors and metastases could be found, indicating that the presence of NY-BR-1 in metastases can be deduced from their corresponding primary. Forty-three paired biopsies taken from patients before and after chemotherapy suggest that NY-BR-1 expression is not influenced by preceding chemotherapy (kappa = 0.89, P < 0.0001). In summary, the co-expression of NY-BR-1 with HLA class I antigens and its expression in metastases without modification by chemotherapy suggest that NY-BR-1 targeted immunotherapy represents a viable strategy in addition to other targeted cancer drug therapies of breast cancer.

  16. 重组腺病毒载体pAd-NY-ESO-1-EGFP的构建及鉴定%Construction and Identification of Recombinant Adenovirns Vector Containing NY-ESO-1 Gene

    Institute of Scientific and Technical Information of China (English)

    张又莉; 徐少勇; 王家宁; 黄永章; 王斌; 宋军; 郭凌郧

    2008-01-01

    目的:利用细菌内同源重组法构建含人肿瘤特异性抗原的腺病毒质粒.方法:设计NY-ESO-1 cDNA扩增引物,从pETl5b-NY-ESO-1质粒中扩增NY-ESO-1的DNA序列,与pShuttle-IRES-hrGFP-2进行连接,PCR和EcoRV酶切鉴定重组质粒pShuttle-NY-ESO-1-EGFP,经Pme I酶切线性化后,转化含pAdeasy-1的超感受态BJ5183大肠杆菌,细菌内同源重组法构建腺病毒质粒pAd-NY-ESO-1-EGFP;质粒经Pac I酶切鉴定,DNA测序.结果:线性化的pShuttle-NY-ESO-1-EGFP转化含pAdeasy-1的超感受态BJ5183大肠杆菌,重组质粒经酶切获得一大于23 kb的大片段和4.5kb的片段,PCR反应扩增出了340 bp的片段.结论:用细菌内同源重组法成功地构建了含NY-ESO-1的重组腺病毒质粒,为进行表达NY-ESO-1的重组腺病毒的制备及肿瘤特异性抗原NY-ESO-1的核酸疫苗在肿瘤方面的研究奠定了基础.

  17. MoS2 nanoflowers as high performance saturable absorbers for an all-fiber passively Q-switched erbium-doped fiber laser.

    Science.gov (United States)

    Wei, Rongfei; Zhang, Hang; Tian, Xiangling; Qiao, Tian; Hu, Zhongliang; Chen, Zhi; He, Xin; Yu, Yongze; Qiu, Jianrong

    2016-04-14

    Strong saturable absorption was observed in MoS2 nanoflowers, which were synthesized by a facile solvothermal method. A MoS2 nanoflower-based saturable absorber with a high modulation depth of 51.8% and a large saturable intensity of 275.5 GW cm(-2) was introduced to the application of passively Q-switched fiber laser generation. Stable passively Q-switched fiber laser pulses at 1.56 μm with a low threshold power of 16.10 mW, high signal-to-noise ratio of 52.5 dB and short pulse duration of 1.9 μs were obtained. More importantly, a high output power of 3.10 mW related to a large pulse energy of about 51.84 nJ can be attained at a very low pump power. The efficiency of the laser reaches 4.71%, which is larger than that of the prepared layered MoS2 and recently reported MoS2-based passively Q-switching operations. Such results imply that the MoS2 nanoflowers are an excellent candidate for a saturable absorber in passively Q-switched fiber lasers at a low pump intensity.

  18. Measurement of target and double-spin asymmetries for the e<mo>→>p<mo>→mo><mo>→mo>eπ<mo>+mo><mo>(n)> reaction in the nucleon resonance region at low Q2

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, X.; Adhikari, K. P.; Bosted, P.; Deur, A.; Drozdov, V.; El Fassi, L.; Kang, Hyekoo; Kovacs, K.; Kuhn, S.; Long, E.; Phillips, S. K.; Ripani, M.; Slifer, K.; Smith, L. C.; Adikaram, D.; Akbar, Z.; Amaryan, M. J.; Anefalos Pereira, S.; Asryan, G.; Avakian, H.; Badui, R. A.; Ball, J.; Baltzell, N. A.; Battaglieri, M.; Batourine, V.; Bedlinskiy, I.; Biselli, A. S.; Briscoe, W. J.; Bültmann, S.; Burkert, V. D.; Carman, D. S.; Celentano, A.; Chandavar, S.; Charles, G.; Chen, J. -P.; Chetry, T.; Choi, Seonho; Ciullo, G.; Clark, L.; Colaneri, L.; Cole, P. L.; Compton, N.; Contalbrigo, M.; Crede, V.; D' Angelo, A.; Dashyan, N.; De Vita, R.; De Sanctis, E.; Djalali, C.; Dodge, G. E.; Dupre, R.; Egiyan, H.; El Alaoui, A.; Elouadrhiri, L.; Eugenio, P.; Fanchini, E.; Fedotov, G.; Fersch, R.; Filippi, A.; Fleming, J. A.; Gevorgyan, N.; Ghandilyan, Y.; Gilfoyle, G. P.; Giovanetti, K. L.; Girod, F. X.; Gleason, C.; Golovach, E.; Gothe, R. W.; Griffioen, K. A.; Guidal, M.; Guler, N.; Guo, L.; Hanretty, C.; Harrison, N.; Hattawy, M.; Hicks, K.; Holtrop, M.; Hughes, S. M.; Ilieva, Y.; Ireland, D. G.; Ishkhanov, B. S.; Isupov, E. L.; Jenkins, D.; Jiang, H.; Jo, H. S.; Joosten, S.; Keller, D.; Khachatryan, G.; Khandaker, M.; Kim, A.; Kim, W.; Klein, F. J.; Kubarovsky, V.; Lanza, L.; Lenisa, P.; Livingston, K.; MacGregor, I. J. D.; Markov, N.; McKinnon, B.; Mirazita, M.; Mokeev, V.; Movsisyan, A.; Munevar, E.; Munoz Camacho, C.; Murdoch, G.; Nadel-Turonski, P.; Net, L. A.; Ni, A.; Niccolai, S.; Niculescu, G.; Niculescu, I.; Osipenko, M.; Ostrovidov, A. I.; Paolone, M.; Paremuzyan, R.; Park, K.; Pasyuk, E.; Peng, P.; Pisano, S.; Pogorelko, O.; Price, J. W.; Puckett, A. J. R.; Raue, B. A.; Rizzo, A.; Rosner, G.; Rossi, P.; Roy, P.; Sabatié, F.; Salgado, C.; Schumacher, R. A.; Sharabian, Y. G.; Skorodumina, Iu.; Smith, G. D.; Sokhan, D.; Sparveris, N.; Stankovic, I.; Strakovsky, I. I.; Strauch, S.; Taiuti, M.; Tian, Ye; Ungaro, M.; Voskanyan, H.; Voutier, E.; Walford, N. K.; Watts, D. P.; Wei, X.; Weinstein, L. B.; Wood, M. H.; Zachariou, N.; Zhang, J.; Zonta, I.

    2016-10-01

    We report measurements of target- and double-spin asymmetries for the exclusive channel e<mo>→>p<mo>→mo><mo>→mo>eπ<mo>+mo><mo>(n)> in the nucleon resonance region at Jefferson Lab using the CEBAF Large Acceptance Spectrometer (CLAS). These asymmetries were extracted from data obtained using a longitudinally polarized NH3 target and a longitudinally polarized electron beam with energies 1.1, 1.3, 2.0, 2.3, and 3.0 GeV. The new results are consistent with previous CLAS publications but are extended to a low Q2 range from 0.0065 to 0.35 (GeV/c)2. The Q2 access was made possible by a custom-built Cherenkov detector that allowed the detection of electrons for scattering angles as low as 6 degrees. These results are compared with the unitary isobar models JANR and MAID, the partial-wave analysis prediction from SAID, and the dynamic model DMT. In many kinematic regions our results, in particular results on the target asymmetry, help to constrain the polarization-dependent components of these models.

  19. Preclinical safety pharmacology study of a novel protein-based cancer vaccine CHP-NY-ESO-1.

    Science.gov (United States)

    Harada, Naozumi; Hoshiai, Kiyotaka; Takahashi, Yoshiyasu; Sakaguchi, Yasue; Kuno, Takayoshi; Hishida, Tadashi; Shiku, Hiroshi

    2008-05-23

    CHP-NY-ESO-1 is a novel therapeutic cancer vaccine consisting of a recombinant protein of cancer antigen NY-ESO-1 and a polysaccharide-based delivery system, cholesteryl pullulan. A pilot clinical study of CHP-NY-ESO-1 in cancer patients was previously conducted, and the adverse events related to this drug were observed to be limited to skin reactions at injection sites. To further establish the safety of CHP-NY-ESO-1, we studied the effects of its subcutaneous injection on vital functions such as the central nervous system, cardiovascular system and respiratory system using preclinical animal models. The effects of CHP-NY-ESO-1 on the cardiovascular system were investigated in dogs using a telemetry system for blood pressure and heart rate and the Holter monitoring for ambulatory electrocardiograms. No drug-related changes were observed in these parameters. The effect of CHP-NY-ESO-1 on the hERG-dependent potassium currents was also examined using in vitro cultured cell system, and no inhibition of hERG currents was observed. The effects of CHP-NY-ESO-1 on the central nervous system were examined in rats using functional observational battery method, and no drug-related changes were observed in home cage observations, open field observations, hand held observations, and perception and motor function observations. The effect of CHP-NY-ESO-1 on the respiratory system was investigated in rats by measuring tidal volume, minute volume and respiratory rate using whole-body plethysmograph method, and no significant changes were found in these parameters. These results indicate that CHP-NY-ESO-1 would not have any pharmacological effects on vital functions and support the safety of this cancer vaccine for clinical use.

  20. Enhancing whole-tumor cell vaccination by engaging innate immune system through NY-ESO-1/dendritic cell interactions.

    Science.gov (United States)

    Xu, Le; Zheng, Junying; Nguyen, David H; Luong, Quang T; Zeng, Gang

    2013-10-01

    NY-ESO-1 is a cancer/germline antigen (Ag) with distinctively strong immunogenicity. We have previously demonstrated that NY-ESO-1 serves as an endogenous adjuvant by engaging dendritic cell (DC)-surface receptors of calreticulin (CRT) and toll-like receptor (TLR) 4. In the present study, NY-ESO-1 was investigated for its immunomodulatory roles as a molecular adjuvant in whole-tumor cell vaccines using the Renca kidney cancer model. Renca cells were genetically engineered to express NY-ESO-1 on the cell surface to enhance direct interactions with DC. The effect of ectopic cell-surface expression of NY-ESO-1 was investigated on tumor immunogenicity, DC activation, cytotoxic T lymphocytes against model tumor-associated Ags, and the effectiveness of the modified tumor cells as a therapeutic whole-cell vaccine. Cell-surface expression of NY-ESO-1 was able to reduce the tumor growth of Renca cells in BALB/c mice, although the modification did not alter cell proliferation rate in vitro. Directly engaging the innate immune system through NY-ESO-1 facilitated the interaction of tumor cells with DC, leading to enhanced DC activation and subsequent tumor-specific T-cell priming. When used as a therapeutic whole-cell vaccine, Renca cells with NY-ESO-1 on the surface mediated stronger inhibitory effects on tumor growth and metastasis compared with parental Renca or Renca cells expressing a control protein GFP on the surface. Augmented antitumor efficacy correlated with increased CD8 T-cell infiltration into tumors and decreased myeloid-derived suppressor cells and regulatory T cells in the spleen. As a cancer/germline Ag and as an immunomodulatory adjuvant through engaging innate immune receptors, NY-ESO-1 offers a unique opportunity for improved whole-tumor cell vaccinations upon the classic GM-CSF-engineered cell vaccines.

  1. NY-ESO-1 is a ubiquitous immunotherapeutic target antigen for patients with myxoid/round cell liposarcoma.

    Science.gov (United States)

    Pollack, Seth M; Jungbluth, Achim A; Hoch, Benjamin L; Farrar, Erik A; Bleakley, Marie; Schneider, David J; Loggers, Elizabeth T; Rodler, Eve; Eary, Janet F; Conrad, Ernest U; Jones, Robin L; Yee, Cassian

    2012-09-15

    Myxoid/round cell liposarcoma (MRCL) is the second most common liposarcoma subtype, accounting for >33% of liposarcomas and approximately 10% of all soft tissue sarcomas. Although MRCL is a chemosensitive subtype, patients with metastatic disease have a poor outcome. NY-ESO-1 is a cancer-testis antigen (also known as cancer germ cell antigen) that has been successfully targeted in vaccine trials and in adoptive T-cell therapy trials for the treatment of several solid tumors. The authors investigated the feasibility of targeting NY-ESO-1 in patients with MRCL by evaluating the prevalence of NY-ESO-1 expression in tumors using immunohistochemistry and quantitative reverse transcriptase-polymerase chain reaction analysis. NY-ESO-1-specific tumor recognition by NY-ESO-1-specific T-cells also was analyzed using a chromium release assay. A search of the University of Washington Sarcoma Tissue Bank identified paraffin-embedded tumor samples from 25 patients with MRCL. NY-ESO-1 expression was observed in every MRCL tumor assessed (100%); in 18 tumors (72%), staining was homogenous. In all but 2 tumors, staining was sufficiently robust (2+) that such patients would be eligible for clinical trials of NY-ESO-1-directed therapy. By using NY-ESO-1 specific, CD8-positive T-cells, the in vitro sensitivity of myxoid liposarcoma cell lines to antigen-specific lysis was demonstrated. The current results establish NY-ESO-1 as an important target antigen for the treatment of patients with MRCL. Copyright © 2012 American Cancer Society.

  2. Effector memory and central memory NY-ESO-1-specific re-directed T cells for treatment of multiple myeloma.

    Science.gov (United States)

    Schuberth, P C; Jakka, G; Jensen, S M; Wadle, A; Gautschi, F; Haley, D; Haile, S; Mischo, A; Held, G; Thiel, M; Tinguely, M; Bifulco, C B; Fox, B A; Renner, C; Petrausch, U

    2013-04-01

    The cancer-testis antigen NY-ESO-1 is a potential target antigen for immune therapy expressed in a subset of patients with multiple myeloma. We generated chimeric antigen receptors (CARs) recognizing the immunodominant NY-ESO-1 peptide 157-165 in the context of HLA-A*02:01 to re-direct autologous CD8(+) T cells towards NY-ESO-1(+) myeloma cells. These re-directed T cells specifically lysed NY-ESO-1(157-165)/HLA-A*02:01-positive cells and secreted IFNγ. A total of 40% of CCR7(-) re-directed T cells had an effector memory phenotype and 5% a central memory phenotype. Based on CCR7 cell sorting, effector and memory CAR-positive T cells were separated and CCR7(+) memory cells demonstrated after antigen-specific re-stimulation downregulation of CCR7 as sign of differentiation towards effector cells accompanied by an increased secretion of memory signature cytokines such as IL-2. To evaluate NY-ESO-1 as potential target antigen, we screened 78 bone marrow biopsies of multiple myeloma patients where NY-ESO-1 protein was found to be expressed by immunohistochemistry in 9.7% of samples. Adoptively transferred NY-ESO-1-specific re-directed T cells protected mice against challenge with endogenously NY-ESO-1-positive myeloma cells in a xenograft model. In conclusion, re-directed effector- and central memory T cells specifically recognized NY-ESO-1(157-165)/ HLA-A*02:01-positive cells resulting in antigen-specific functionality in vitro and in vivo.

  3. Distinct expression patterns of the immunogenic differentiation antigen NY-BR-1 in normal breast, testis and their malignant counterparts.

    Science.gov (United States)

    Theurillat, Jean-Philippe; Zürrer-Härdi, Ursina; Varga, Zsuzsanna; Barghorn, André; Saller, Elisabeth; Frei, Claudia; Storz, Martina; Behnke, Silvia; Seifert, Burkhardt; Fehr, Mathias; Fink, Daniel; Rageth, Christoph; Linsenmeier, Claudia; Pestalozzi, Bernhard; Chen, Yao-Tseng; Knuth, Alexander; Jäger, Dirk; Moch, Holger

    2008-04-01

    NY-BR-1 is a differentiation antigen and a potential target for cancer immunotherapy. Its mRNA expression is restricted to breast, testis, prostate and breast cancer by RT-PCR. In this study, we correlated NY-BR-1 protein and mRNA expression on tissue microarrays of mammary, prostatic and testicular malignancies using immunohistochemistry and in situ hybridization with probes for exon 4-7 and 30-33. NY-BR-1 mRNA was confined to primary spermatocytes, suggesting a role in spermatogenesis. Exon 4-7 and 30-33 were equally expressed this cell type. However, NY-BR-1 was absent in all germ cell tumours analyzed (n = 475) and present in one of 56 (2%) prostate carcinomas. In breast, NY-BR-1 mRNA expression was detected in 307 of 442 (70%) primary carcinomas, with strong correlation to its protein expression (p < 0.0001). mRNA expression was significantly stronger and more frequently detected by the exon 30-33 probe than by the exon 4-7 probe (70% vs. 35%, p < 0.0001), indicating the presence of alternative splice variants that lack 5-prime sequences. A similar restricted mRNA pattern was also observed in the normal breast epithelium. NY-BR-1 protein and mRNA correlated significantly with estrogen receptor alpha (ER alpha) protein expression (p < 0.0001), with stronger association to NY-BR-1 mRNA than protein (odds ratio 7.7 compared to 4.6). We identified 4 estrogen response elements (ERE)-like sequences nearby the promoter region, suggesting that NY-BR-1 transcription might be controlled by ER alpha. Accordingly, analysis of matching pairs of primary tumors with their recurrences showed a marked decrease of NY-BR-1 expression in recurrences after tamoxifen treatment (p < 0.0001).

  4. Pr16Mo21O56

    Directory of Open Access Journals (Sweden)

    Patrick Gougeon

    2011-05-01

    Full Text Available The structure of hexadecapraseodymium henicosamolybdenum hexapentacontaoxide, Pr16Mo21O56, is isotypic with other rare earth representatives of formula type RE16Mo21O56 (RE = La, Ce, Nd. It is characterized by Mo10O18iO8a units (where i = inner and a = apical O atoms containing bioctahedral Mo10 clusters and octahedral MoO6 units that share some of their O atoms to form the Mo–O framework. The two independent Mo10 cluster units are centred at Wyckoff positions 2b and 2c and have point-group symmetry overline{1}. The Mo atom of the MoO6 unit is likewise situated at an inversion centre (2d. The eight crystallographically different Pr3+ cations occupy irregular voids in the framework with coordination numbers to the O atoms varying between 8 and 11.

  5. Strategies for bird conservation: The Partners in Flight planning process; Proceedings of the 3rd Partners in Flight Workshop; 1995 October 1-5; Cape May, NJ

    Science.gov (United States)

    Rick Bonney; David N. Pashley; Robert J. Cooper; Larry Niles

    2000-01-01

    This volume represents a compilation of papers presented at the 3rd International Partners in Flight Workshop held October 1-5, 1995, at the Grand Hotel in Cape May, NJ. The title of the workshop was "Partners in Flight Conservation Plan: Building Consensus for Action." Manuscripts have been available on-line at the Cornell Laboratory of Ornithology web site...

  6. Northern Saw-whet Owls (Aegolius acadicus) captured at Cape May Point, NJ, 1980-1994: comparison of two capture techniques

    Science.gov (United States)

    Katharine E. Duffy; Patrick E. Matheny

    1997-01-01

    During autumn migration 1980-1994, 1,270 Northern Saw-whet Owls (Aegolius acadicus) (NSWO) were captured and banded at Cape May Point, NJ. From 1980-1988, captures were effected by passive mist-netting. From 1989-1994, an audiolure (NSWO territorial song broadcast loudly from dusk to dawn in the trapping area) was used to enhance capture rate. 638...

  7. 76 FR 4284 - Foreign-Trade Zone 49-Newark, NJ Area; Application for Subzone; LVMH Watch & Jewelry U.S.A., Inc...

    Science.gov (United States)

    2011-01-25

    ... & Jewelry U.S.A., Inc. (Watches, Jewelry Products and Leather Goods); Springfield, NJ An application has... LVMH Watch & Jewelry U.S.A., Inc. (LVMH), located in Springfield, New Jersey. The application was..., jewelry products, leather goods (apparel, hand bags, wallets, cases), accessories, and luggage. All of...

  8. A 158 fs 5.3 nJ fiber-laser system at 1 mu m using photonic bandgap fibers for dispersion control and pulse compression

    DEFF Research Database (Denmark)

    Nielsen, C.K.; Jespersen, Kim Giessmann; Keiding, S.R.

    2006-01-01

    We demonstrate a 158 fs 5.3 nJ mode-locked laser system based on a fiber oscillator, fiber amplifier and fiber compressor. Dispersion compensation in the fiber oscillator was obtained with a solid-core photonic bandgap (SC-PBG) fiber spliced to standard fibers, and external compression is obtaine...

  9. Optimization of the Processing of Mo Disks

    Energy Technology Data Exchange (ETDEWEB)

    Tkac, Peter [Argonne National Lab. (ANL), Argonne, IL (United States); Rotsch, David A. [Argonne National Lab. (ANL), Argonne, IL (United States); Stepinski, Dominique [Argonne National Lab. (ANL), Argonne, IL (United States); Makarashvili, Vakhtang [Argonne National Lab. (ANL), Argonne, IL (United States); Harvey, James [NorthStar Medical Technologies, LLC, Madison, WI (United States); Vandegrift, George F. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2016-01-01

    The objective of this work is to decrease the processing time for irradiated disks of enriched Mo for the production of 99Mo. Results are given for the dissolution of nonirradiated Mo disks, optimization of the process for large-scale dissolution of sintered disks, optimization of the removal of the main side products (Zr and Nb) from dissolved targets, and dissolution of irradiated Mo disks.

  10. MAGE-A3/4 and NY-ESO-1 antigens expression in metastatic esophageal squamous cell carcinoma.

    Science.gov (United States)

    Bujas, T; Marusic, Z; Peric Balja, M; Mijic, A; Kruslin, B; Tomas, D

    2011-03-21

    In the present study we analyzed immunohistochemical expression of MAGE-A 3/4 and NY-ESO-1 in 55 samples of esophageal squamous cell carcinomas (ESCC) and their respective lymph node metastases. To our knowledge this is the first study to assess and compare the expression of these antigens in ESCC lymph node metastases. Fifty (90.9%) primary ESCC were positive for MAGE-A 3/4 and 53 (96.6%) were positive for NY-ESO-1. MAGE-A 3/4 was expressed in all lymph node metastases and the intensity of expression was high in a majority of cases. NY-ESO-1 was negative in 2 (7.1%) lymph nodes metastases, while the reaction was predominantly moderate in the positive group. In primary tumors MAGE-A 3/4 showed a significantly higher intensity of expression compared to NY-ESO-1 (P=0.047), while in lymph node metastases the intensity of expression was not significantly different (P=0.387). Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression. Intensity of MAGE-A 3/4 (P=0.461) and NY-ESO-1 (P=0.414) expression in primary tumors was not significantly different compared to the expression in their respective lymph nodes metastases. Expression of MAGE-A 3/4 in primary tumors showed significant positive correlation with primary tumor expression of NY-ESO-1 (P=0.021) but no significant correlation with the expression of MAGE-A 3/4 in lymph node metastases (P=0.056). Expression of NY-ESO-1 in primary tumors showed significant positive correlation with the expression of NY-ESO-1 in lymph node metastases (P=0.001) and significant negative correlation with patients’ age (P<0.001). Expression of MAGE-A 3/4 and NY-ESO-1 in primary tumors and lymph node metastases showed no significant correlation with prognostic parameters such as tumor grade and TNM stage (P>0.05). We have shown different levels of MAGE-A 3/4 and NY-ESO-1 expression in almost all specimens of primary tumor and lymph node metastases

  11. QTAIM analysis of the bonding in Mo-Mo bonded dimolybdenum complexes.

    Science.gov (United States)

    Van der Maelen, Juan F; Cabeza, Javier A

    2012-07-02

    A number of local and integral topological parameters of the electron density of relevant bonding interactions in the binuclear molybdenum complexes [Mo(2)Cl(8)](4-), [Mo(2)(μ-CH(3)CO(2))(4)], [Mo(2)(μ-CF(3)CO(2))(4)], [Mo(2)(μ-CH(3)CO(2))(4)Br(2)](2-), [Mo(2)(μ-CF(3)CO(2))(4)Br(2)](2-), [Mo(2)(μ-CH(3)CO(2))(2)Cl(4)](2-), [Mo(2)(μ-CH(3)CO(2))(2)(μ-Cl)(2)Cl(4)](2-), and [Mo(2)(μ-Cl)(3)Cl(6)](3-) have been calculated and interpreted under the perspective of the quantum theory of atoms in molecules (QTAIM). These data have allowed a comparison between related but different atom-atom interactions, such as different Mo-Mo formal bond orders, ligand-unbridged versus Cl-bridged, CH(3)CO(2)-bridged, and CF(3)CO(2)-bridged Mo-Mo interactions, and Mo-Cl(terminal) and Mo-Cl(bridge) versus Mo-Br and Mo-O interactions. Calculations carried out using nonrelativistic and relativistic approaches afforded similar results.

  12. Expression and clinical significance of NY-ESO-1 protein in human laryngeal squamous carcinoma%NY-ESO-1蛋白在喉鳞状细胞癌中表达的意义

    Institute of Scientific and Technical Information of China (English)

    田俊; 王斌全; 李建民; 皇甫辉; 温树信; 张艳廷

    2010-01-01

    目的 检测NY-ESO-1蛋白在喉鳞癌中的表达情况,探讨其作为喉癌免疫治疗靶标的实验依据及其与喉癌生物学行为的关系.方法 免疫组织化学PV-9000两步法检测69例喉鳞癌患者原发灶及其常规病理报告为阳性的121个颈淋巴结中NY-ESO-1的表达,同时采用Western blotting方法检测其在喉鳞癌标本的癌中心区、癌周0.5、1.0 cm及9例全喉切除术患者的喉正常黏膜组织(对照)中的表达.结果 69例喉鳞癌组织中,NY-ESO-1蛋白表达阳性率为43.48%(30/69),在癌中心区、癌周0.5、1.0cm中的表达逐渐下降(P<0.05),NY-ESO-1在对照组喉正常黏膜组织中无表达.NY-ESO-1蛋白在不同T分级、病理分级和不同颈淋巴结转移喉癌中的表达率差异无统计学意义(P>0.05),颈转移淋巴结中NY-ESO-1的表达下调.结论 NY-ESO-1在喉鳞癌患者中特异性高表达,可能以某种方式参与了喉癌的发生、发展,是否能将其作为喉癌免疫治疗靶标有待深入研究.%Objective To detect the expression of Cancer-Testis antigen NY-ESO-1 in human laryngeal squamous carcinoma (LSC) and to explore its significance in immunotherapeutic application. Methods The expressions of NY-ESO-1 protein in the LSC and in the pathologically positive lymph nodes were detected by PV-9000 Immunohistochemistry. Western blotting was also employed to measure the expressions of NY-ESO-1 in the tumor core region(TC), the tissues at the sites of 0.5cm, 1.0cm away from LSC periphery and the distant normal larynx tissues. Results NY-ESO-1 protein expression was positive in 30 out of 69 (43.48 %) cases of LSC. The expression level of NY-ESO-1 protein were found to significantly decrease by tums in TC and corresponding adjacent tissues (P <0.01). None of the nine normal larynx tissues expressed NY-ESO-1 protein.It did not display an obvious correlation between the expression of NY-ESO-1 with T staging, pathological grading and lymph node metastasis (P

  13. NY-ESO-1 Protein Cancer Vaccine With Poly-ICLC and OK-432: Rapid and Strong Induction of NY-ESO-1-specific Immune Responses by Poly-ICLC.

    Science.gov (United States)

    Takeoka, Tomohira; Nagase, Hirotsugu; Kurose, Koji; Ohue, Yoshihiro; Yamasaki, Makoto; Takiguchi, Shuji; Sato, Eiichi; Isobe, Midori; Kanazawa, Takayuki; Matsumoto, Mitsunobu; Iwahori, Kota; Kawashima, Atsunari; Morimoto-Okazawa, Akiko; Nishikawa, Hiroyoshi; Oka, Mikio; Pan, Linda; Venhaus, Ralph; Nakayama, Eiichi; Mori, Masaki; Doki, Yuichiro; Wada, Hisashi

    2017-03-23

    We conducted a clinical trial of a cancer vaccine using NY-ESO-1 protein with polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) and/or OK-432 against solid tumors. A total of 15 patients were sequentially enrolled in 4 cohorts. Patients in cohort 1 received NY-ESO-1 protein; cohort 2a received NY-ESO-1 protein+OK-432; cohort 2b received NY-ESO-1 protein+poly-ICLC; cohort 3 received NY-ESO-1 protein+OK-432+poly-ICLC with Montanide ISA-51. The endpoints of this trial were safety, NY-ESO-1 immune responses, and clinical response. Vaccine-related adverse events observed were fever and injection-site reaction (grade 1). Two patients showed stable disease after vaccination. NY-ESO-1 antibodies were observed in 4 patients at the baseline (sero-positive) and augmented in all patients after vaccination. Eleven patients showed a conversion of negative antibody responses at baseline to positive after vaccination (seroconversion). The seroconversions were observed in all 11 sero-negative patients by the fourth immunization; in particular, it was observed by the second immunization in patients with poly-ICLC, and these induced antibody responses were stronger than those in patients immunized without poly-ICLC. The number of NY-ESO-1-specific interferon (IFN)γ-producing T cells was increased in patients immunized with poly-ICLC and/or OK-432, and furthermore, the increase of IFNγ-producing CD8 T cells in patients immunized with poly-ICLC was significantly higher than that in patients without poly-ICLC. Nonspecific activations of T-cell or antigen presenting cells were not observed. Our present study showed that poly-ICLC is a promising adjuvant for cancer vaccines.

  14. Molecular cloning, prokaryotic expression and purification of human CT antigen NY-ESO-1%CT抗原NY-ESO-1基因克隆、表达及纯化

    Institute of Scientific and Technical Information of China (English)

    江华; 郑玉玲; 姜永强

    2007-01-01

    目的:利用大肠杆菌表达人CT抗原NY-ESO-1,对表达产物进行Western印迹鉴定和纯化,为今后利用其进行肿瘤疫苗的研究奠定基础.方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白.结果与结论:拼接的NY-ESO-1全基因经测序证明与GenBank中人的NY-ESO-l全基因完全相符;构建的原核表达载体NY-ESO-1-pET28a(+)可稳定地可溶性表达NY-ESO-1蛋白;经Ni柱亲和纯化获得较好的纯化结果;利用鼠抗人单克隆抗体对表达的蛋白进行验证,结果显示阳性.本研究成功利用原核表达系统实现了对CT抗原NY-ESO-1的可溶性表达.

  15. Synthesis of hierarchical MoO2/MoS2 nanofibers for electrocatalytic hydrogen evolution

    Science.gov (United States)

    Rheem, Youngwoo; Han, Yosep; Lee, Kyu Hwan; Choi, Sung-Mook; Myung, Nosang V.

    2017-03-01

    Perpendicularly attached MoS2 nanosheets on MoO2 conductive nanofibers were synthesized by combining electrospinning, calcination, and sulfurization processes. Compared to randomly stacked MoS2 nanosheets on MoO2 nanofiber, they show greater hydrogen evolution reaction (HER) performance (i.e., onset potential of ‑180 mV versus normal hydrogen electrode with the Tafel slope of 59 mV dec‑1). HER performance decreases with increasing MoS2 nanocrystal size.

  16. Chemical and geochemical composition of spring-summer Arctic aerosol collected at Ny Alesund, Svalbard Islands.

    Science.gov (United States)

    Udisti, Roberto; Becagli, Silvia; Caiazzo, Laura; Cappelletti, David; Giardi, Fabio; Grotti, Marco; Lucarelli, Franco; Moroni, Beatrice; Nava, Silvia; Severi, Mirko; Traversi, Rita

    2017-04-01

    Since March 2010, spring-summer (usually March - September) campaigns were continuously carried out at the Italian Gruvebadet Observatory, Ny Alesund, Svalbard Island. Aerosol was sampled by PM10 (daily) and 4-stage (4-day resolution) collector devices and size distribution was evaluated at 10 min resolution in the range 10 nm - 20 um (106 size classes by a TSI SMPS-APS integrated system). Six-year (2010-2015) PM10 and size-segregated (>10, 10-2.5, 2.5-1, 10.1016/j.atmosenv.2016.04.002. A. Bazzano et al., "Long-range transport of atmospheric lead reaching Ny Alesund: inter-annual and seasonal variations of potential source areas". Atmos. Environ., 2016, 139, 11-19. http://dx.doi.org/10.1016/j.atmosenv.2016.05.026.

  17. Ny krigsførelse i informationsalderen? Om ‘Revolution in Military Affairs’ og Irakkrigen

    Directory of Open Access Journals (Sweden)

    Jens Claus Hansen

    2005-09-01

    Full Text Available Artiklen diskuterer, hvorvidt man efter Golfkrigen kan tale om en ‘Revolu- tion in Military Affairs’ – RMA – som nogle amerikanske krigsteoretikere hævder har fundet sted med anvendelsen af ny teknologi i moderne krigs- førelse. Forfatteren viser, hvordan moderne krigsstrategier har inkorpore- ret ny teknologi og mediebrug som bl.a. informationskrig, ‘Systems of Sys- tems’ og Network Centric Warfare. Forfatteren påpeger, at den strategiske anvendelse af informationsoperationer – herunder ‘embedded’ journalister – ikke har medført en demokratisering af nyhedsdækningen og heller ikke indebærer en øget sandhedsværdi. Afslutningsvis peger forfatteren på behovet for en ‘Revolution in Diplomatic Affairs’.

  18. Characteristics of carbonaceous aerosol in a two-week campaign at Ny-(A)lesund

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The concentrations of organic carbon (OC) and elemental carbon (EC)in total suspended particle (TSP) were investigated at Ny-(A)lesund, Svalbard in a two-week campaign. The levels of OC and EC are 0.86 ±0.27 μgm-3 (mean±standard deviation) and 0. 19±0.10 μgm-3 , respectively. Back trajectory analysis of air masses arriving at Ny-(A)lesund reveals that long-range transport of polluted air play insignificant role in OC and EC levels, to which the potential influence of the local contamination were ascribed. The average OC/EC ratio is 5.41,suggesting the presence of the secondary organic aerosols. The estimated secondary organic carbon (SOC) in TSP is 0.59 μg/m3 , accounting for 64% of the total organic carbon.

  19. CIRCUMBINARY PLANETS ORBITING THE RAPIDLY PULSATING SUBDWARF B-TYPE BINARY NY Vir

    Energy Technology Data Exchange (ETDEWEB)

    Qian, S.-B.; Zhu, L.-Y.; Dai, Z.-B.; He, J.-J. [National Astronomical Observatories/Yunnan Observatory, Chinese Academy of Sciences (CAS), P.O. Box 110, 650011 Kunming (China); Fernandez-Lajus, E. [Facultad de Ciencias Astronomicas y Geofisicas, Universidad Nacional de La Plata, 1900 La Plata, Buenos Aires (Argentina); Xiang, F.-Y., E-mail: qsb@ynao.ac.cn [Physics Department, Xiangtan University, 411105 Xiangtan, Funan Province (China)

    2012-02-15

    We report here the tentative discovery of a Jovian planet in orbit around the rapidly pulsating subdwarf B-type (sdB-type) eclipsing binary NY Vir. By using newly determined eclipse times together with those collected from the literature, we detect that the observed-calculated (O - C) curve of NY Vir shows a small-amplitude cyclic variation with a period of 7.9 yr and a semiamplitude of 6.1 s, while it undergoes a downward parabolic change (revealing a period decrease at a rate of P-dot = -9.2 x 10{sup -12}). The periodic variation was analyzed for the light-travel-time effect via the presence of a third body. The mass of the tertiary companion was determined to be M{sub 3}sin i' = 2.3({+-} 0.3)M{sub Jupiter} when a total mass of 0.60 M{sub Sun} for NY Vir is adopted. This suggests that it is most probably a giant circumbinary planet orbiting NY Vir at a distance of about 3.3 astronomical units (AU). Since the rate of period decrease cannot be explained by true angular momentum loss caused by gravitational radiation or/and magnetic braking, the observed downward parabolic change in the O - C diagram may be only a part of a long-period (longer than 15 years) cyclic variation, which may reveal the presence of another Jovian planet ({approx}2.5 M{sub Jupiter}) in the system.

  20. Immunohistochemical assessment of NY-ESO-1 expression in esophageal adenocarcinoma resection specimens.

    Science.gov (United States)

    Hayes, Stephen J; Hng, Keng Ngee; Clark, Peter; Thistlethwaite, Fiona; Hawkins, Robert E; Ang, Yeng

    2014-04-14

    To assess NY-ESO-1 expression in a cohort of esophageal adenocarcinomas. A retrospective search of our tissue archive for esophageal resection specimens containing esophageal adenocarcinoma was performed, for cases which had previously been reported for diagnostic purposes, using the systematised nomenclature of human and veterinary medicine coding system. Original haematoxylin and eosin stained sections were reviewed, using light microscopy, to confirm classification and tumour differentiation. A total of 27 adenocarcinoma resection specimens were then assessed using immunohistochemistry for NY-ESO-1 expression: 4 well differentiated, 14 moderately differentiated, 4 moderate-poorly differentiated, and 5 poorly differentiated. Four out of a total of 27 cases of esophageal adenocarcinoma examined (15%) displayed diffuse cytoplasmic and nuclear expression for NY-ESO-1. They displayed a heterogeneous and mosaic-type pattern of diffuse staining. Diffuse cytoplasmic staining was not identified in any of these structures: stroma, normal squamous epithelium, normal submucosal gland and duct, Barrett's esophagus (goblet cell), Barrett's esophagus (non-goblet cell) and high grade glandular dysplasia. All adenocarcinomas showed an unexpected dot-type pattern of staining at nuclear, paranuclear and cytoplasmic locations. Similar dot-type staining, with varying frequency and size of dots, was observed on examination of Barrett's metaplasia, esophageal submucosal gland acini and the large bowel negative control, predominantly at the crypt base. Furthermore, a prominent pattern of apical (luminal) cytoplasmic dot-type staining was observed in some cases of Barrett's metaplasia and also adenocarcinoma. A further morphological finding of interest was noted on examination of haematoxylin and eosin stained sections, as aggregates of lymphocytes were consistently noted to surround submucosal glands. We have demonstrated for the first time NY-ESO-1 expression by esophageal

  1. Designprocesser og arbejdsmiljø: Udvikling af ny teknologi til fødevareindustrien

    DEFF Research Database (Denmark)

    Broberg, Ole

    1999-01-01

    Dette papir handler om muligheder og betingelser for at inddrage arbejdsmiljøhensyn i designprocesser. Baggrunden for det er, at jeg har haft mulighed for at følge udviklingen af en ny maskine til fødevareindustrien næsten lige fra de grundlæggende idéer blev udviklet og foreløbig frem til i dag,...

  2. A novel murine T-cell receptor targeting NY-ESO-1.

    Science.gov (United States)

    Rosati, Shannon F; Parkhurst, Maria R; Hong, Young; Zheng, Zhili; Feldman, Steven A; Rao, Mahadev; Abate-Daga, Daniel; Beard, Rachel E; Xu, Hui; Black, Mary A; Robbins, Paul F; Schrump, David A; Rosenberg, Steven A; Morgan, Richard A

    2014-04-01

    Cancer testis antigens, such as NY-ESO-1, are expressed in a variety of prevalent tumors and represent potential targets for T-cell receptor (TCR) gene therapy. DNA encoding a murine anti-NY-ESO-1 TCR gene (mTCR) was isolated from immunized HLA-A*0201 transgenic mice and inserted into a γ-retroviral vector. Two mTCR vectors were produced and used to transduce human PBL. Transduced cells were cocultured with tumor target cell lines and T2 cells pulsed with the NY-ESO-1 peptide, and assayed for cytokine release and cell lysis activity. The most active TCR construct was selected for production of a master cell bank for clinical use. mTCR-transduced PBL maintained TCR expression in short-term and long-term culture, ranging from 50% to 90% efficiency 7-11 days after stimulation and 46%-82% 10-20 days after restimulation. High levels of interferon-γ secretion were observed (1000-12000 pg/mL), in tumor coculture assays and recognition of peptide-pulsed cells was observed at 0.1 ng/mL, suggesting that the new mTCR had high avidity for antigen recognition. mTCR-transduced T cells also specifically lysed human tumor targets. In all assays, the mTCR was equivalent or better than the comparable human TCR. As the functional activity of TCR-transduced cells may be affected by the formation of mixed dimers, mTCRs, which are less likely to form mixed dimers with endogenous hTCRs, may be more effective in vivo. This new mTCR targeted to NY-ESO-1 represents a novel potential therapeutic option for adoptive cell-transfer therapy for a variety of malignancies.

  3. Formulation of Organic Fertilizer Standard in Jiaxing City Based on NY525-2012

    Institute of Scientific and Technical Information of China (English)

    Jinfa; HUANG; Xiongwei; NI; Jianping; XIE; Yanping; SHI

    2013-01-01

    On the basis of research, analysis and argumentation, we conduct analysis and research on the current situation of quality of commercial organic fertilizer in Jiaxing City, and carry out the inspection and testing of organic matter indicators using the laboratory. Based on the standard of NY525-2012 Organic Fertilizer by the Ministry of Agriculture, this paper formulates the quality level indicators of local commercial organic fertilizer and adding specifications of raw materials.

  4. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  5. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  6. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY...

  7. Kate Bárány: a life of science, teaching, and service.

    Science.gov (United States)

    Solaro, R John; Rao, Mrinalini C

    2012-12-01

    We celebrate the lives of Michael and Kate Bárány in this issue of the Journal of Muscle Research and Cell Motility. Kate and Michael died within weeks of each other in 2011. Joe Chalovich has written about Michael and we write about Kate. As emphasized by Joe, Kate, and Michael were remarkable individuals who survived the Holocaust, the Hungarian revolution, and emerged from as much adversity as one might imagine to become productive scientists, educators, citizens, and symbols of the durability of the human spirit. They present their own story in an essay (Bárány and Bárány 2000) published in a monograph "Selected Topics in the History of Biochemistry." Rather than repeating much of the list of scientific achievements chronicled in these papers, we focus here on Kate, especially in her role as an individual and partner in science, while at the same time being an accomplished teacher, and a champion of women in science.

  8. Prompt Gamma Activation Analysis of the Nyírlugos obsidian core depot find

    Directory of Open Access Journals (Sweden)

    Zsolt Kasztovszky

    2014-03-01

    Full Text Available The Nyírlugos obsidian core depot find is one of the most important lithic assemblages in the collection of the Hungarian National Museum (HNM. The original set comprised 12 giant obsidian cores, of which 11 are currently on the permanent archaeological exhibition of the HNM. One of the cores is known to be inDebrecen. The first publication attributed the hoard, on the strength of giant (flint blades known from the Early and Middle Copper Age Tiszapolgár and Bodrogkeresztúr cultures, to the Copper Age. In the light of recent finds it is more likely to belong to the Middle Neolithic period. The source area was defined as Tokaj Mts., about100 kmto the NW from Nyírlugos. The size and beauty of the exceptional pieces exclude any invasive analysis. Using Prompt Gamma Activation Analysis (PGAA, we can measure major chemical components and some key trace elements of stone artefacts with adequate accuracy to successfully determine provenance of obsidian. Recent methodological development also facilitated the study of relatively large objects like the Nyírlugos cores. The cores were individually measured by PGAA. The results show that the cores originate from the Carpathian 1 sources, most probably the Viničky variety (C1b. The study of the hoard as a batch is an important contribution to the assessment of prehistoric trade and allows us to reconsider the so-called Carpathian, especially Carpathian 1 (Slovakian sources.

  9. Resiquimod as an Immunologic Adjuvant for NY-ESO-1 Protein Vaccination in Patients with High Risk Melanoma

    Science.gov (United States)

    Gnjatic, Sacha; Cruz, Crystal M.; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P.; Rolnitzsky, Linda; Goldberg, Judith; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A.; Bhardwaj, Nina

    2015-01-01

    The TLR7/8 agonist, Resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide with or without Resiquimod in high-risk melanoma patients. In Part I of the study, patients received 100ug full length NY-ESO-1 protein emulsified in 1.25mL Montanide (day 1) followed by topical application of 1000mg of 0.2% Resiquimod gel on days 1 and 3 (Cohort 1) versus days 1, 3, and 5 (Cohort 2) of a 21 day cycle. In Part II, patients were randomized to receive 100ug NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel (Arm-A; N=8) or 1000mg of 0.2% Resiquimod gel (Arm-B; N=12) using the dosing regimen established in Part I. The vaccine regimens were generally well-tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In Part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4+ T-cell responses. CD8+ T-cell responses were only seen in 3 of 12 patients in Arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8+ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical Resiquimod is safe and induces both antibody and CD4+ T-cell responses in the majority of patients; the small proportion of CD8+ T-cell responses suggests that the addition of topical Resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8+ T-cell responses. PMID:25633712

  10. NY-ESO-1在喉鳞状细胞癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    周慧; 凌红阳; 吴文璎; 郑玉双

    2015-01-01

    目的 探讨喉鳞状细胞癌组织中NY-ESO-1蛋白表达及其临床意义.方法 免疫组化方法检测88例喉鳞状细胞癌组织(A组)和10例声带息肉组织(B组)NY-ESO-1蛋白的表达.A组再分为四个亚组,临床分期Ⅰ、Ⅱ期38例(A1组),临床分期Ⅲ、Ⅳ期50例(A2组);病理类型高、中分化60例(A3组),低分化28例(A4组).结果 A组NY-ESO-1蛋白表达水平高于B组(4598±2302 vs.0)(P<0.01).A1组NY-ESO-1表达水平高于A2组(8052±3810 vs.162±107)(P<0.05).A3组NY-ESO-1表达水平高于A4组(5090±1893 vs.211±201) (P<0.05).结论 NY-ESO-1有可能作为喉鳞癌早期诊断的新指标;NY-ESO-1表达阳性的高、中分化喉鳞癌患者可能可以选择NY-ESO-1疫苗作为辅助治疗.

  11. MoS2 spaser

    Science.gov (United States)

    Jayasekara, Charith; Premaratne, Malin; Gunapala, Sarath D.; Stockman, Mark I.

    2016-04-01

    We present a comprehensive analysis of a spaser made of a circular shaped highly doped molybdenum disulfide (MoS2) resonator. "Spaser" is an acronym for "surface plasmon amplification by stimulated emission of radiation"-a nanoscale source of surface plasmons generated by stimulated emission in a plasmonic resonator which receives energy nonradiatively. By considering localized surface plasmon modes, operation characteristics of the model are analysed, and tunability of the design is demonstrated. We find the optimum geometric and material parameters of the spaser that provides efficient outputs and carryout a comparative analysis with a similar circular spaser made of graphene. Owing to physical and chemical properties of MoS2 and the active medium, the proposed design delivers efficient outputs in terms of spaser mode energy, operating thresholds, Q-factor, and electric field amplitude. Lower operating thresholds and higher mode energies are notable advantages of the design. Owing to having many superior features to existing similar designs, this MoS2 spaser may be much suited for applications in nanoplasmonic devices.

  12. Magnetic properties of Nd(Fe1-xCox)10.5M1.5 (M=Mo and V) and their nitrides

    Science.gov (United States)

    Fu, J. B.; Yu, X.; Qi, Z. Q.; Yang, W. Y.; Liu, S. Q.; Wang, C. S.; Du, H. L.; Han, J. Z.; Yang, Y. C.; Yang, J. B.

    2017-05-01

    In this work, alloys of Nd(Fe1-xCox)10.5M1.5 (M=Mo and V) were prepared via arc melting and heat treatment. The nitrides of these alloys were synthesized using a gas-solid state reaction method. The influence of Co substitution for Fe in NdFe10.5Mo1.5 and NdFe10.5V1.5 alloys and their nitrides were investigated. It was found that the lattice parameters a, c, and unit cell volume V decrease with increasing Co content x for Nd(Fe1-xCox)10.5Mo1.5. As compared to their parent alloys, the lattice parameters and unit cells volume increase after nitrogenation, which gives rise to higher Curie temperature, magnetization and magnetocrystalline anisotropy field for nitrides. A small amount of Co substitution for Fe (x≤0.3) can enhance the magnetic properties including Curie temperature, saturation magnetization and magnetocrystalline anisotropy field of the alloys and their nitrides, while higher concentration of Co (x>0.3) will deteriorates these magnetic properties, especially for the nitrides, due to the modification of the band structure by Co atom. As a result, Nd(Fe1-xCox)10.5Mo1.5Ny and Nd(Fe1-xCox)10.5V1.5Ny with x≤0.2 become promising candidates for permanent magnet applications. A coercivity of 4.6 kOe and maximum energy product BHmax of 20 MGOe were achieved for NdFe9.45Co1.05Mo1.5Nx.

  13. MoSi2 oxidation resistance coatings for Mo5Si3/MoSi2 composites

    Institute of Scientific and Technical Information of China (English)

    YAN Jianhui; XU Hongmei; ZHANG Houan; TANG Siwen

    2009-01-01

    In order to improve the oxidation resistance properties of 30 at.% Mo5Si3/MoSi2 composite at high temperature in air, a molybdenum disili-tide coating was prepared on its surface by a molten salt technology. XRD and SEM analysis showed that only tetragonal MoSi2 phase ex-isted in the coating after being siliconized for 5 h at 900℃. The oxidation film formed on the uncoated sample was not dense, so that oxygen diffused easily through it. The volatilization of MoO3 resulted in the oxidation film separating from the substrate. The MoSi2coating was proved to be an effective method to prevent 30 at.% MosSi3/MoSi2 composites from being oxidized at 1200℃. A dense glassy SiO2 film was formed on the MoSi2 coating surface, which acted as a barrier layer for the diffusion of oxygen atoms to the substrate. The 30at.% Mo5Si3/MoSi2 composites with a MoSi2 coating showed much better oxidation resistance at high temperature.

  14. Immune escape from NY-ESO-1-specific T-cell therapy via loss of heterozygosity in the MHC.

    Science.gov (United States)

    Klippel, Z K; Chou, J; Towlerton, A M; Voong, L N; Robbins, P; Bensinger, W I; Warren, E H

    2014-03-01

    Adoptive immunotherapy of tumors with T cells specific for the cancer-testis antigen NY-ESO-1 has shown great promise in preclinical models and in early stage clinical trials. Tumor persistence or recurrence after NY-ESO-1-specific therapy occurs, however, and the mechanisms of recurrence remain poorly defined. In a murine xenograft model of NY-ESO-1(+) multiple myeloma, we observed tumor recurrence after adoptive transfer of CD8(+) T cells genetically redirected to the prototypic NY-ESO-1157-165 peptide presented by HLA-A*02:01. Analysis of the myeloma cells that had escaped from T-cell control revealed intact expression of NY-ESO-1 and B2M, but selective, complete loss of HLA-A*02:01 expression from the cell surface. Loss of heterozygosity (LOH) in the major histocompatibility complex (MHC) involving the HLA-A locus was identified in the tumor cells, and further analysis revealed selective loss of the allele encoding HLA-A*02:01. Although LOH involving the MHC has not been described in myeloma patients with persistent or recurrent disease after immune therapies such as allogeneic hematopoietic cell transplantation (HCT), it has been described in patients with acute myelogenous leukemia who relapsed after allogeneic HCT. These results suggest that MHC loss should be evaluated in patients with myeloma and other cancers who relapse after adoptive NY-ESO-1-specific T-cell therapy.

  15. A cancer vaccine induces expansion of NY-ESO-1-specific regulatory T cells in patients with advanced melanoma.

    Science.gov (United States)

    Ebert, Lisa M; MacRaild, Sarah E; Zanker, Damien; Davis, Ian D; Cebon, Jonathan; Chen, Weisan

    2012-01-01

    Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg), which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIX™ cancer vaccine. All patients tested had Treg (CD25(bright) FoxP3(+) CD127(neg)) specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1(157-170) epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1(115-132) peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials.

  16. Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8(+) T cells.

    Science.gov (United States)

    Pollack, Seth M; Jones, Robin L; Farrar, Erik A; Lai, Ivy P; Lee, Sylvia M; Cao, Jianhong; Pillarisetty, Venu G; Hoch, Benjamin L; Gullett, Ashley; Bleakley, Marie; Conrad, Ernest U; Eary, Janet F; Shibuya, Kendall C; Warren, Edus H; Carstens, Jason N; Heimfeld, Shelly; Riddell, Stanley R; Yee, Cassian

    2014-01-01

    Adoptive T cell therapy represents an attractive modality for the treatment of patients with cancer. Peripheral blood mononuclear cells have been used as a source of antigen specific T cells but the very low frequency of T cells recognizing commonly expressed antigens such as NY-ESO-1 limit the applicability of this approach to other solid tumors. To overcome this, we tested a strategy combining IL-21 modulation during in vitro stimulation with first-in-class use of tetramer-guided cell sorting to generate NY-ESO-1 specific cytotoxic T lymphocytes (CTL). CTL generation was evaluated in 6 patients with NY-ESO-1 positive sarcomas, under clinical manufacturing conditions and characterized for phenotypic and functional properties. Following in vitro stimulation, T cells stained with NY-ESO-1 tetramer were enriched from frequencies as low as 0.4% to >90% after single pass through a clinical grade sorter. NY-ESO-1 specific T cells were generated from all 6 patients. The final products expanded on average 1200-fold to a total of 36 billion cells, were oligoclonal and contained 67-97% CD8(+), tetramer(+) T cells with a memory phenotype that recognized endogenous NY-ESO-1. This study represents the first series using tetramer-guided cell sorting to generate T cells for adoptive therapy. This approach, when used to target more broadly expressed tumor antigens such as WT-1 and additional Cancer-Testis antigens will enhance the scope and feasibility of adoptive T cell therapy.

  17. Factors Influencing Divergent Patterns of Phosphorus Availability in NY and PA Biogeochemical `Hotspots'

    Science.gov (United States)

    Saia, S. M.; Hofmeister, K.; Regan, J. M.; Buda, A. R.; Carrick, H. J.; Walter, M. T.

    2016-12-01

    Anthropogenic alteration of the soil phosphorus (P) cycle leads to subsequent water quality issues in agricultural dominated watersheds. In the humid Northeastern United States (NE US), variably saturated areas can generate surface runoff that transports P and stimulates biogeochemical processes; these hydrologically dynamic locations are often called biogeochemical `hotspots'. Many studies have evaluated nitrogen and carbon cycling in biogeochemical hot spots but few have focused on P. We hypothesized seasonally wet parts of the landscape (i.e., hotspots) have smaller biologically available P pools because runoff events frequently carry away nutrients like P. To test this hypothesis, we generated soil wetness index (SWI) maps from soil (SURRGO) and elevation (LiDAR rescaled to 3 m) data and used these maps to direct seasonal soil sampling near Klingerstown, Pennsylvania (PA) and Ithaca, New York (NY). We collected 5cm deep soil samples in PA (bimonthly) and NY (monthly) along soil moisture gradients for a range of land cover types (forest, fallow, and cropped) from May through October. We measured soil moisture in the field and percent organic matter (OM), pH, and three increasingly strong soil P extractions (dilute-salt-extractable P, oxalate-extractable P, and total-extractable P) in the laboratory. Our results indicated a negative relationship between dilute-salt-extractable P concentrations and SWI in PA and no relationship between these same variables in NY. We also found positive relationships between each of the three P extractions in PA but only a positive relationship between oxalate-extractable P and total-extractable P in NY. Our findings in PA support our hypothesis; namely, less biologically available P (i.e. dilute-salt-extractable P) is found in wetter areas of the landscape. However, divergent P availability patterns in NY point to further complexities and confounding variables in our understanding in soil P processes. Further studies will look

  18. New high-pressure phases of MoSe2 and MoTe2

    Science.gov (United States)

    Kohulák, Oto; MartoÅák, Roman

    2017-02-01

    Three Mo-based transition-metal dichalcogenides MoS2,MoSe2, and MoTe2 share at ambient conditions the same structure 2 Hc , consisting of layers where Mo atoms are surrounded by six chalcogen atoms in trigonal prism coordination. The knowledge of their high-pressure behavior is, however, limited, particularly in case of MoSe2 and MoTe2. The latter materials do not undergo a layer-sliding transition 2 Hc→ 2 Ha known in MoS2 and currently no other stable phase aside from 2 Hc is known in these systems at room temperature. Employing evolutionary crystal structure prediction in combination with ab initio calculations, we study the zero-temperature phase diagram of both materials up to Mbar pressures. We find a tetragonal phase with space group P4/mmm, previously predicted in MoS2, to become stable in MoSe2 at 118 GPa. In MoTe2, we predict at 50 GPa a transition to a new layered tetragonal structure with space group I4/mmm, similar to CaC2, where Mo atoms are surrounded by eight Te atoms. The phase is metallic already at the transition pressure and becomes a good metal beyond 1 Mbar. We discuss chemical trends in the family of Mo-based transition-metal dichalcogenides and suggest that MoTe2 likely offers the easiest route towards the post-2 H phases.

  19. Differential Insulin Secretion of High-Fat Diet-Fed C57BL/6NN and C57BL/6NJ Mice: Implications of Mixed Genetic Background in Metabolic Studies.

    Directory of Open Access Journals (Sweden)

    Camille Attané

    Full Text Available Many metabolic studies employ tissue-specific gene knockout mice, which requires breeding of floxed gene mice, available mostly on C57BL/6N (NN genetic background, with cre or Flp recombinase-expressing mice, available on C57BL/6J (JJ background, resulting in the generation of mixed C57BL/6NJ (NJ genetic background mice. Recent awareness of many genetic differences between NN and JJ strains including the deletion of nicotinamide nucleotide transhydrogenase (nnt, necessitates examination of the consequence of mixed NJ background on glucose tolerance, beta cell function and other metabolic parameters. Male mice with NN and NJ genetic background were fed with normal or high fat diets (HFD for 12 weeks and glucose and insulin homeostasis were studied. Genotype had no effect on body weight and food intake in mice fed normal or high fat diets. Insulinemia in the fed and fasted states and after a glucose challenge was lower in HFD-fed NJ mice, even though their glycemia and insulin sensitivity were similar to NN mice. NJ mice showed mild glucose intolerance. Moreover, glucose- but not KCl-stimulated insulin secretion in isolated islets was decreased in HFD-fed NJ vs NN mice without changes in insulin content and beta cell mass. Under normal diet, besides reduced fed insulinemia, NN and NJ mice presented similar metabolic parameters. However, HFD-fed NJ mice displayed lower fed and fasted insulinemia and glucose-induced insulin secretion in vivo and ex vivo, as compared to NN mice. These results strongly caution against using unmatched mixed genetic background C57BL/6 mice for comparisons, particularly under HFD conditions.

  20. Efficient in vivo priming by vaccination with recombinant NY-ESO-1 protein and CpG in antigen naive prostate cancer patients.

    Science.gov (United States)

    Karbach, Julia; Neumann, Antje; Atmaca, Akin; Wahle, Claudia; Brand, Kathrin; von Boehmer, Lotta; Knuth, Alexander; Bender, Armin; Ritter, Gerd; Old, Lloyd J; Jäger, Elke

    2011-02-15

    NY-ESO-1, one of the most immunogenic tumor antigens, is expressed in 15% to 25% of metastatic prostate cancers. The immunological and clinical effects of vaccination with recombinant NY-ESO-1 protein combined with CpG as adjuvant were evaluated. In a phase I clinical study, patients with advanced prostate cancer were vaccinated with recombinant NY-ESO-1 protein (100 μg) mixed with CpG 7909 (2.5 mg) every 3 weeks intradermally for 4 doses. Objectives of the study were the safety of the vaccine and changes of specific humoral and cellular immunological responses to NY-ESO-1 in relation to detectable NY-ESO-1 expression in the individual tumor. All 12 baseline sero-negative patients developed high-titer NY-ESO-1 antibody responses. B-cell epitope mapping identified NY-ESO-1 p91-110 to be recognized most frequently by vaccine-induced antibodies. Two patients developed significant antibody titers against the adjuvant CpG. NY-ESO-1-specific CD4+ and/or CD8+ T-cell responses were induced in 9 patients (69%). Five of these 9 patients did not express NY-ESO-1 in the autologous tumor. Postvaccine CD8+ T-cell clones recognized and lyzed HLA-matched tumor cell lines in an antigen-specific manner. Our data provide clear evidence for the capacity of NY-ESO-1 protein/CpG vaccine to induce integrated antigen-specific immune responses in vivo and to efficiently prime CD8+ T-cell responses in NY-ESO-1 antigen-negative patients. Our results may also support further clinical vaccination protocols with NY-ESO-1 protein not only focused on the treatment of existing cancer, but also to prevent further development of NY-ESO-1 positive cancers in vivo. ©2010 AACR.

  1. Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients.

    Science.gov (United States)

    Kageyama, Shinichi; Wada, Hisashi; Muro, Kei; Niwa, Yasumasa; Ueda, Shugo; Miyata, Hiroshi; Takiguchi, Shuji; Sugino, Sahoko H; Miyahara, Yoshihiro; Ikeda, Hiroaki; Imai, Naoko; Sato, Eiichi; Yamada, Tomomi; Osako, Masaharu; Ohnishi, Mami; Harada, Naozumi; Hishida, Tadashi; Doki, Yuichiro; Shiku, Hiroshi

    2013-10-05

    Cholesteryl pullulan (CHP) is a novel antigen delivery system for cancer vaccines. This study evaluated the safety, immune responses and clinical outcomes of patients who received the CHP-NY-ESO-1 complex vaccine, Drug code: IMF-001. Patients with advanced/metastatic esophageal cancer were enrolled and subcutaneously vaccinated with either 100 μg or 200 μg of NY-ESO-1 protein complexed with CHP. The primary endpoints were safety and humoral immune responses, and the secondary endpoint was clinical efficacy. A total of 25 patients were enrolled. Thirteen and twelve patients were repeatedly vaccinated with 100 μg or 200 μg of CHP-NY-ESO-1 with a median of 8 or 9.5 doses, respectively. No serious adverse events related to the vaccine were observed. Three out of 13 patients in the 100-μg cohort and 7 out of 12 patients in the 200-μg cohort were positive for anti-NY-ESO-1 antibodies at baseline. In the 100-μg cohort, an antibody response was observed in 5 out of 10 pre-antibody-negatives patients, and the antibody levels were augmented in 2 pre-antibody-positive patients after vaccination. In the 200-μg cohort, all 5 pre-antibody-negative patients became seropositive, and the antibody level was amplified in all 7 pre-antibody-positive patients. No tumor shrinkage was observed. The patients who received 200 μg of CHP-NY-ESO-1 survived longer than patients receiving 100 μg of CHP-NY-ESO-1, even those who exhibited unresponsiveness to previous therapies or had higher tumor burdens. The safety and immunogenicity of CHP-NY-ESO-1 vaccine were confirmed. The 200 μg dose more efficiently induced immune responses and suggested better survival benefits. (Clinical trial registration number NCT01003808).

  2. Induction of CD8 T-cell responses restricted to multiple HLA class I alleles in a cancer patient by immunization with a 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide.

    Science.gov (United States)

    Eikawa, Shingo; Kakimi, Kazuhiro; Isobe, Midori; Kuzushima, Kiyotaka; Luescher, Immanuel; Ohue, Yoshihiro; Ikeuchi, Kazuhiro; Uenaka, Akiko; Nishikawa, Hiroyoshi; Udono, Heiichiro; Oka, Mikio; Nakayama, Eiichi

    2013-01-15

    Immunogenicity of a long 20-mer NY-ESO-1f peptide vaccine was evaluated in a lung cancer patient TK-f01, immunized with the peptide with Picibanil OK-432 and Montanide ISA-51. We showed that internalization of the peptide was necessary to present CD8 T-cell epitopes on APC, contrasting with the direct presentation of the short epitope. CD8 T-cell responses restricted to all five HLA class I alleles were induced in the patient after the peptide vaccination. Clonal analysis showed that B*35:01 and B*52:01-restricted CD8 T-cell responses were the two dominant responses. The minimal epitopes recognized by A*24:02, B*35:01, B*52:01 and C*12:02-restricted CD8 T-cell clones were defined and peptide/HLA tetramers were produced. NY-ESO-1 91-101 on A*24:02, NY-ESO-1 92-102 on B*35:01, NY-ESO-1 96-104 on B*52:01 and NY-ESO-1 96-104 on C*12:02 were new epitopes first defined in this study. Identification of the A*24:02 epitope is highly relevant for studying the Japanese population because of its high expression frequency (60%). High affinity CD8 T-cells recognizing tumor cells naturally expressing the epitopes and matched HLA were induced at a significant level. The findings suggest the usefulness of a long 20-mer NY-ESO-1f peptide harboring multiple CD8 T-cell epitopes as an NY-ESO-1 vaccine. Characterization of CD8 T-cell responses in immunomonitoring using peptide/HLA tetramers revealed that multiple CD8 T-cell responses comprised the dominant response. Copyright © 2012 UICC.

  3. 靶向克隆法构建NY-ESO-1基因的原核表达载体%Construction of NY-ESO-1 Gene Prokaryotic Expression Vector by LP Recco PCR Cloning

    Institute of Scientific and Technical Information of China (English)

    俞远东; 徐少勇; 王斌; 王家宁; 黄永章

    2009-01-01

    目的:利用PCR产物靶向克隆法构建人癌-睾丸抗原NY-ESO-1的原核表达载体.方法:从人新鲜的食管癌组织中提取总RNA,通过RT-PCR技术扩增NY-ESO-1基因3'端的340 bp片段,采用靶向克隆法将目的基因插入到原核表达载体pET-15b中,得到重组表达质粒pET-15b-NY-ESO-1,经过筛选,挑选出阳性克隆进行Xho Ⅰ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等,鉴定所构建的原核表达载体.结果:扩增得到NY-ESO-1基因片段,经测序证实与GenBank公布的序列一致,重组的质粒经过PCR,酶切鉴定获得了一个大小为340 bp的特征性片段,证明重组质粒中已成功的插入了目的基因NY-ESO-1.结论:靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体,为制备pET-15b-NY-ESO-1融合蛋白奠定了基础.

  4. Novel Approach for Isolation and Identification of Porcine Epidemic Diarrhea Virus (PEDV) Strain NJ Using Porcine Intestinal Epithelial Cells

    Science.gov (United States)

    Shi, Wen; Jia, Shuo; Zhao, Haiyuan; Yin, Jiyuan; Wang, Xiaona; Yu, Meiling; Ma, Sunting; Wu, Yang; Chen, Ying; Fan, Wenlu; Xu, Yigang; Li, Yijing

    2017-01-01

    Porcine epidemic diarrhea virus (PEDV), which is the causative agent of porcine epidemic diarrhea in China and other countries, is responsible for serious economic losses in the pork industry. Inactivated PEDV vaccine plays a key role in controlling the prevalence of PEDV. However, consistently low viral titers are obtained during the propagation of PEDV in vitro; this represents a challenge to molecular analyses of the virus and vaccine development. In this study, we successfully isolated a PEDV isolate (strain NJ) from clinical samples collected during a recent outbreak of diarrhea in piglets in China, using porcine intestinal epithelial cells (IEC). We found that the isolate was better adapted to growth in IECs than in Vero cells, and the titer of the IEC cultures was 104.5 TCID50/0.1 mL at passage 45. Mutations in the S protein increased with the viral passage and the mutations tended towards attenuation. Viral challenge showed that the survival of IEC-adapted cultures was higher at the 45th passage than at the 5th passage. The use of IECs to isolate and propagate PEDV provides an effective approach for laboratory-based diagnosis of PEDV, as well as studies of the epidemiological characteristics and molecular biology of this virus. PMID:28117718

  5. Electron scattering from 92Mo

    Science.gov (United States)

    Milliman, T. E.; Connelly, J. P.; Heisenberg, J. H.; Hersman, F. W.; Wise, J. E.; Papanicolas, C. N.

    1990-06-01

    Differential cross sections for electron scattering from 92Mo have been measured for excitation energies less than 5.1 MeV over a range of momentum transfer of 0.5 to 3.1 fm-1. The elastic scattering data are analyzed along with existing electron and muonic atom data to provide an improved description of the ground-state charge distribution. The inelastic scattering data have been analyzed to extract electromagnetic transition densities. These densities are interpreted in terms of the underlying nuclear structure.

  6. Olympus Turbo MO 640SII

    Institute of Scientific and Technical Information of China (English)

    蔡翰林

    2001-01-01

    @@ 此款由Olympus推出的TurboMO 640 SII,外观上,以全机身乳白色的优美色调,配合简洁的控制面板;规格上,可读取最大达640MB容量的磁盘片并可向下兼容.在众多的硬件中又多了一台美观实用的MO光盘机可供使用者选择.

  7. Combination of autoantibodies against NY-ESO-1 and viral capsid antigen immunoglobulin A for improved detection of nasopharyngeal carcinoma.

    Science.gov (United States)

    Peng, Yu-Hui; Xu, Yi-Wei; Qiu, Si-Qi; Hong, Chao-Qun; Zhai, Tian-Tian; Li, En-Min; Xu, Li-Yan

    2014-09-01

    Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southern China and Southeast Asia, and early detection remains a challenge. Autoantibodies have been found to precede the manifestations of symptomatic cancer by several months to years, making their identification of particular relevance for early detection. In the present study, the diagnostic value of serum autoantibodies against NY-ESO-1 in NPC patients was evaluated. The study included 112 patients with NPC and 138 normal controls. Serum levels of autoantibodies against NY-ESO-1 and classical Epstein-Barr virus marker, viral capsid antigen immunoglobulin A (VCA-IgA), were measured by enzyme-linked immunosorbent assay. Measurement of autoantibodies against NY-ESO-1 and VCA-IgA demonstrated a sensitivity/specificity of 42.9/94.9% [95% confidence interval (CI), 33.7-52.6/89.4-97.8%] and 55.4/95.7% (95% CI, 45.7-64.7/90.4-98.2%), respectively. The area under receiver operating characteristic curve for autoantibodies against NY-ESO-1 (0.821; 95% CI, 0.771-0.871) was marginally lower than that for VCA-IgA (0.860; 95% CI, 0.810-0.910) in NPC. The combination of autoantibodies against NY-ESO-1 and VCA-IgA yielded an enhanced sensitivity of 80.4% (95% CI, 71.6-87.0%) and a specificity of 90.6% (95% CI, 84.1-94.7%). Moreover, detection of autoantibodies against NY-ESO-1 could differentiate early-stage NPC patients from normal controls. Our results suggest that autoantibodies against NY-ESO-1 may serve as a potential biomarker, as a supplement to VCA-IgA, for the screening and diagnosis of NPC.

  8. Cancer-testis antigen expression in synovial sarcoma: NY-ESO-1, PRAME, MAGEA4, and MAGEA1.

    Science.gov (United States)

    Iura, Kunio; Maekawa, Akira; Kohashi, Kenichi; Ishii, Takeaki; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Harimaya, Katsumi; Iwamoto, Yukihide; Oda, Yoshinao

    2017-03-01

    Synovial sarcoma (SS) is regarded as a relatively chemosensitive sarcoma, but the prognosis of advanced SSs remains poor. Here we identified highly expressed cancer-testis antigens that could be promising immunotherapy targets for SS, using a previously conducted cDNA microarray, and we assessed the clinicopathological or prognostic relationships of these antigens in SS. We compared the gene expression profiles of 11 SSs with those of 3 normal adipose tissues. Among the up-regulated cancer-testis antigens, we analyzed PRAME, MAGEA1, and MAGEA4 and another cancer-testis antigen (NY-ESO-1) together, by immunohistochemistry and real-time polymerase chain reaction in 108 SSs. Immunohistochemically, NY-ESO-1, PRAME, MAGEA4, and MAGEA1 were positive in 66 (61%), 93 (86%), 89 (82%), and 16 (15%) of 108 SSs, respectively, and 104 (96%) of 108 SSs showed the immunohistochemical expression of at least 1 of NY-ESO-1, PRAME, and MAGEA4. Moreover, the high expression of at least 1 of these 3 antigens was observed in 83% of the SSs. High expression of NY-ESO-1 and MAGEA4 was significantly correlated with the presence of necrosis and advanced clinical stage. The immunohistochemical expression of these cancer-testis antigens was not correlated with prognosis, but the coexpression of NY-ESO-1, PRAME, and MAGEA4 was significantly associated with adverse prognosis. The real-time polymerase chain reaction results were closely related to the immunohistochemical results: NY-ESO-1 (P = .0019), PRAME (P = .039), MAGEA4 (P = .0149), and MAGEA1 (P = .0766). These data support the potential utility of NY-ESO-1, PRAME, and MAGEA4 as immunotherapy targets and ancillary prognostic parameters, suggesting the possible benefit of the combined use of these cancer-testis antigens as an SS immunotherapy target. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation.

    Science.gov (United States)

    Luetkens, Tim; Kobold, Sebastian; Cao, Yanran; Ristic, Marina; Schilling, Georgia; Tams, Sinje; Bartels, Britta Marlen; Templin, Julia; Bartels, Katrin; Hildebrandt, York; Yousef, Sara; Marx, Andreas; Haag, Friedrich; Bokemeyer, Carsten; Kröger, Nicolaus; Atanackovic, Djordje

    2014-11-01

    Multiple myeloma (MM) is the malignancy with the most frequent expression of the highly immunogenic cancer-testis antigens (CTA), and we have performed the first analysis of longitudinal expression, immunological properties, and fine specificity of CTA-specific antibody responses in MM. Frequency and characteristics of antibody responses against cancer-testis antigens MAGE-A3, NY-ESO-1, PRAME, and SSX-2 were analyzed using peripheral blood (N = 1094) and bone marrow (N = 200) plasma samples from 194 MM patients. We found that antibody responses against CTA were surprisingly rare, only 2.6 and 3.1 % of patients evidenced NY-ESO-1- and SSX-2-specific antibodies, respectively. NY-ESO-1-specific responses were observed during disease progression, while anti-SSX-2 antibodies appeared after allogeneic stem cell transplantation and persisted during clinical remission. We found that NY-ESO-1- and SSX-2-specific antibodies were both capable of activating complement and increasing CTA uptake by antigen-presenting cells. SSX-2-specific antibodies were restricted to IgG3, NY-ESO-1 responses to IgG1 and IgG3. Remarkably, NY-ESO-1-positive sera recognized various non-contiguous regions, while SSX-2-specific responses were directed against a single 6mer epitope, SSX-2(85-90). We conclude that primary autoantibodies against intracellular MM-specific tumor antigens SSX-2 and NY-ESO-1 are rare but functional. While their contribution to disease control still remains unclear, our data demonstrate their theoretic ability to affect cellular anti-tumor immunity by formation and uptake of mono- and polyvalent immune complexes.

  10. Generation of a 2.2 nJ picosecond optical pulse with blue-violet wavelength using a GaInN master oscillator power amplifier

    Science.gov (United States)

    Koda, Rintaro; Takiguchi, Yoshiro; Kono, Shunsuke; Watanabe, Hideki; Hanzawa, Yasunari; Nakajima, Hiroshi; Shiozaki, Masaki; Sugawara, Nobuhiro; Kuramoto, Masaru; Narui, Hironobu

    2015-07-01

    We report the generation of a picosecond optical pulse with 2.2 nJ pulse energy at blue-violet wavelengths using a GaN-based mode-locked laser diode (MLLD) and a semiconductor optical amplifier (SOA). The picosecond optical pulse generated by MLLD at a frequency of 812 MHz was amplified effectively by SOA. We optimized SOA with a widely flared waveguide structure to generate a high optical pulse energy.

  11. Effect of metal ions on fermentation and metabolization of Actinobacillus Succinogenes NJ113%金属离子对产琥珀酸放线杆菌NJ113厌氧发酵代谢的影响

    Institute of Scientific and Technical Information of China (English)

    郑晓宇; 李建; 方晓江; 陈可泉; 奚永兰; 姜岷

    2011-01-01

    The effects of adding Mg2+. Mn2+, Co2+ on cell growth and succinic acid production was investigated. The metabolic flux of Actinobacillus succinogenes NJ113 was calculated. It was found that the flux of HMP increased by 445.38%, 176.23 % and 171.67% after adding 6 mmol/L Mg2+, 6 mmol/L Mn2+, 2 mmol/L Co2+ respectively, thus the reducing power was better balanced. The flux of C4 was 57.70%, 15.94% and 2.91% higher respectively, which led to the improvement of succinic acid flux by 62.69%, 18.91% and 5.01%. The key enzyme activity analysis showed that the specific activity of PEP carboxykinase (Pck) reached 568.732 U/mg, 728.049 U/mg and 339.686 U/mg with 6 mmol/L Mg2+, 6 mmol/L Mn2+, 2 mmol/L Co2+ addition respectively. As a result, the concentration of succinc acid was 27.83 g/L, 26.27 g/L, and 23.54 g/L, while the concentration of control was only 22.79 g/L.%考察了培养基中分别添加Mg2+、Mn2+、Co2+3种金属离子对Actinobacilus Succinogenes NJ113菌体生长及产酸的影响,并进行了代谢通量分析。结果表明培养基中分别添加6mmTo1/LMg2+、6mmol/LMnz+、2mmol/LCo2+后流向HMP途径的通量r17比对照组分别提高了445.38%、176.23%和171.67%,使得还原力不足的矛盾得到缓解;流向C4途径的通量r13比对照组分别提高了57.70%、15.94%和2.91%;最终使得流向丁二酸的通量r16比对照组分别提高了62.69%、18.91%和5.01%。此外,关键酶活分析结果显示分别添加Mg2+、Mn2+以及Co2+后,PEP羧化激酶(Pck)比活力由对照组的339.18U/mg分别提高到568.732U/mg、728.049U/mg和339.686U/mg。最终当培养基中分别添加6mmol/LMg2+、6immol/LMn2+、2immol/LCo2+后丁二酸产量分别为27.83 g/L、26.27 g/L和23.54 g/L,比对照的22.79 g/L分别提高22.11%、15.27%以及3.4%。

  12. Thermodynamic Analysis of Electrodeposited Ni-Mo and Ni-Mo-P Alloys

    Institute of Scientific and Technical Information of China (English)

    GUO Zhong-cheng; XU Rui-dong; WANG Ji-kun

    2004-01-01

    The potential-pH diagram of Ni-P-H2O system is calculated and constructed by use of thermodynamic data. On the basis of the potential-pH diagram the electrochemical behaviors of electrodepositing Ni-Mo and Ni-Mo-P alloys are analyzed. The phases of Ni-Mo and Ni-Mo-P coatings are identified by means of X-ray diffraction analysis. The thermodynamic analysis shows that it is difficult to deposit Mo or P individually from its aqueous solution, and they must be co-deposited with other metals by induced deposition; P and Ni can be deposited as compound Ni3P on the cathode; Mo is deposited in two forms: one is in the form of MoO3 solid particles and the other is in the form of compound MoC. X-ray diffraction analysis is in agreement with the thermodynamic analysis.

  13. NY-ESO-1 Vaccination in Combination with Decitabine Induces Antigen-Specific T-Lymphocyte Responses in Patients with Myelodysplastic Syndrome.

    Science.gov (United States)

    Griffiths, Elizabeth A; Srivastava, Pragya; Matsuzaki, Junko; Brumberger, Zachary; Wang, Eunice S; Kocent, Justin; Miller, Austin; Roloff, Gregory W; Wong, Hong Yuen; Paluch, Benjamin E; Lutgen-Dunckley, Linda G; Martens, Brandon L; Odunsi, Kunle; Karpf, Adam R; Hourigan, Christopher S; Nemeth, Michael J

    2017-09-25

    Treatment options are limited for patients with high-risk myelodysplastic syndrome (MDS). The azanucleosides, azacitidine and decitabine, are front-line therapy for MDS that induce promoter demethylation and gene expression of the highly immunogenic tumor antigen NY-ESO-1. We demonstrated that AML patients receiving decitabine exhibit induction of NY-ESO-1 expression in circulating blasts. We hypothesized that vaccinating against NY-ESO-1 in MDS patients receiving decitabine would capitalize upon induced NY-ESO-1 expression in malignant myeloid cells to provoke an NY-ESO-1-specific-MDS directed cytotoxic T-cell immune response. In a phase I study, 9 MDS patients received an HLA unrestricted NY-ESO-1 vaccine (CDX-1401 + poly-ICLC) in a non-overlapping schedule every four weeks with standard dose decitabine. Analysis of samples serially obtained from the 7 patients who reached the end-of-study demonstrated induction of NY-ESO-1 expression in 7/7 patients and NY-ESO-1 specific CD4+ and CD8+ T-lymphocyte responses in 6/7 and 4/7 of the vaccinated patients respectively. Myeloid cells expressing NY-ESO-1, isolated from a patient at different time-points during decitabine therapy, were capable of activating a cytotoxic response from autologous NY-ESO-1-specific T-lymphocytes. Vaccine responses were associated with a detectable population of CD141Hi conventional dendritic cells, which are critical for the uptake of NY-ESO-1 vaccine and have a recognized role in anti-tumor immune responses. These data indicate that vaccination against induced NY-ESO-1 expression can produce an antigen-specific immune response in a relatively non-immunogenic myeloid cancer and highlight the potential for induced-antigen directed immunotherapy in a group of patients with limited options. Copyright ©2017, American Association for Cancer Research.

  14. EPA Finalizes Plan to Address Contaminated Groundwater at Curtis Specialty Papers Superfund Site in Milford Borough and Alexandria Township, N.J.

    Science.gov (United States)

    (New York, N.Y.) The U.S. Environmental Protection Agency has finalized a plan to clean up contaminated groundwater at the Curtis Specialty Papers Superfund site in Milford Borough and Alexandria Township, New Jersey. The site includes the former Milford P

  15. Mo99 Production Plant Layout

    Energy Technology Data Exchange (ETDEWEB)

    Woloshun, Keith Albert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Dale, Gregory E. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Naranjo, Angela Carol [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-06-25

    The NorthStar Medical Technologies 99Mo production facility configuration is envisioned to be 8 accelerator pairs irradiating 7 100Mo targets (one spare accelerator pair undergoing maintenance while the other 7 pairs are irradiating targets). The required shielding in every direction for the accelerators is initially estimated to be 10 feet of concrete. With the accelerator pairs on one (ground) level and spaced with the required shielding between adjacent pairs, the only practical path for target insertion and removal while minimizing floor space is vertical. The current scheme then requires a target vertical lift of nominally 10 feet through a shield stack. It is envisioned that the lift will be directly into a hot cell where an activated target can be removed from its holder and a new target attached and lowered. The hot cell is on a rail system so that a single hot cell can service all active target locations, as well as deliver the ready targets to the separations lab. On this rail system, coupled to the hot cell, will be a helium recovery and clean-up system. All helium coolant equipment is located on the upper level near to the target removal point.

  16. Håndværk og design; nyt fag, ny didaktik – et aktionsforskningsprojekt

    Directory of Open Access Journals (Sweden)

    Stig Pedersen

    2011-11-01

    Full Text Available Projektet tager afsæt i regeringens forslag om at sammenlægge fagene sløjd og håndarbejde til et nyt fag ”håndværk og design”. Det er projektets ambition at være dagsordensættende i forhold til udvikling af dette nye skolefag. Vi vil arbejde ud fra en aktionsforskningsmæssig tilgang. Det betyder, at vi ikke alene har til hensigt at etablere et ”objektivt” billede af, hvordan udvalgte skoler arbejder med at udvikle det ny fag ”håndværk og design”, men ønsker at påvirke udviklingen og dermed i fællesskab konstruere ny didaktisk teori. Som støtte for projektgruppens arbejde nedsættes en metodisk, didaktisk studiekreds, hvor projektgruppen modtager sparring fra erfarne forskere. Det er projektets sigte at undersøge og udvikle det ny fags didaktik med henblik på at generere visioner for bl.a. indholdsudvælgelse og læreformer i et fremtidsperspektiv for faget ”håndværk og design” i samspil mellem de ideer, viden og erfaringer forskerne har, den viden og erfaring de udvalgte forskende lærere har, samt de erfaringer og ideer, der opbygges i projektets praksisdele. Der arbejdes med eksemplariske forløb, som planlægges og efterbehandles i samarbejde mellem lærere og forskere. Dette samarbejde dokumenteres af lærerne og projektgruppens medlemmer i fællesskab. Her indgår fx undervisningsoplæg, materialer og genstande, evalueringsformer – og resultater samt fokusgruppe interview.Søkord: metod, sløjd, håndværk og design, aktionsforskning, folkeskolenURN:NBN:no-29962

  17. Framtagning av ny lyftytrystning för gasturbin SGT-600/700

    OpenAIRE

    Sjöberg, Emma

    2008-01-01

    I detta Examensarbete har en ny lyftutrustning till gasturbin SGT-600/700 olika moduler tagits fram. Examensarbetet har utförts på Siemens Industrial Turbomachinery AB i Finspång på avdelningen GPTP (leverans gasturbin teknik package installation). Målet med arbetet har varit att skapa en lyftutrustning som är mer flexibel än den befintliga lyftutrustningen och klarar samlyft (lyft med hjälp av två kranar). Arbetet har utförts med hjälp av olika metoder som QFD, funktionsmedel-träd och morpho...

  18. Primes of the form x2+ny2 Fermat, class field theory, and complex multiplication

    CERN Document Server

    Cox, David A

    2014-01-01

    An exciting approach to the history and mathematics of number theory ". . . the author's style is totally lucid and very easy to read . . .the result is indeed a wonderful story." -Mathematical ReviewsWritten in a unique and accessible style for readers of varied mathematical backgrounds, the Second Edition of Primes of the Form p = x2+ ny2 details the history behind how Pierre de Fermat's work ultimately gave birth to quadratic reciprocity and the genus theory of quadratic forms. The book also illustrates how results of Euler and Gauss can be fully understood only in the context of class fi

  19. Boktrailern – : En kartläggning av en ny medieprodukt

    OpenAIRE

    Rybin, Claudia

    2015-01-01

    I detta examensarbete kartlägger jag en ny medieprodukt, boktrailern. En boktrailer är en audiovisuell reklam för en bok och härstammar från samt följer dels samma konventioner som den etablerade filmtrailertraditionen. I vissa fall kan boktrailern även ses som en självständig kortfilm. Boktrailern har uppkommit vid sekelskiftet och utvecklats som en följd av tre faktorer kopplade till tjugohundratalets teknologiska framsteg: 1. Verbal text är allt mer tvungen att förlita sig på ...

  20. Edge termination of MoS2 and CoMoS catalyst particles

    DEFF Research Database (Denmark)

    Byskov, Line Sjolte; Nørskov, Jens Kehlet; Clausen, B. S.;

    2000-01-01

    The edge termination of MoS2 and CoMoS catalyst particles is studied by density functional calculations. We show that for structures without vacancies Mo-terminated edges have the lowest edge energies. Creation of vacancies, which are believed to be active sites in these catalyst systems, leads...

  1. Determination of crystallization as a function of Mo layer thickness in Mo/Si multilayers

    DEFF Research Database (Denmark)

    Abdali, Salim; Gerward, Leif; Yakshin, A.E.;

    2002-01-01

    Mo/Si multilayer samples with different Mo layer thickness were deposited by electron beam evaporation, while Kr+ ions (300 eV) were used for polishing the Si layers. Crystallization as a function of the Mo layer thickness deposited was investigated by grazing incidence X-ray diffraction, giving...

  2. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Science.gov (United States)

    2011-01-01

    Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA) in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis. PMID:22060015

  3. Isolation of a Halophilic Bacterium, Bacillus sp. Strain NY-6 for Organic Contaminants Removal in Saline Wastewater on Ship

    Institute of Scientific and Technical Information of China (English)

    Jie Gao; Zhenjiang Yu; Xiaohui Zhang; Dan Zhao; Fangbo Zhao

    2013-01-01

    The objective of this research was to examine if certain strains of Bacillus bacteria,could survive in dry powder products and if so,could the bacteria degrade organic contaminants in saline wastewater on a ship.As part of the study,we isolated 7 domesticated strains named NY1,NY2,…,and NY7,the strain NY6 showed to have the best performance for organic matter degradation and could survive in dry powder more than 3 months.NY6 was identified as Bacillus aerius,based on the morphological and physic-chemical properties.Its optimal growth conditions were as follows:salinity was 2%; temperature was 37℃; pH was in 6.5-7.0; best ratio of C∶ N∶ P was 100∶5∶1.The capability of its dry powder for Chemical Oxygen Demand (COD) removal was 800mg COD/g in synthesized marine wastewater with 2% salinity.The spores in the dry powder were 1.972× 108 g1.

  4. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Directory of Open Access Journals (Sweden)

    Konkankit Veerauo V

    2011-11-01

    Full Text Available Abstract Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis.

  5. Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells.

    Science.gov (United States)

    Mobergslien, Anne; Vasovic, Vlada; Mathiesen, Geir; Fredriksen, Lasse; Westby, Phuong; Eijsink, Vincent G H; Peng, Qian; Sioud, Mouldy

    2015-01-01

    Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying conserved proteins such as heat shock protein-27 and galectin-1, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-α, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells in-vitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

  6. Progress of NY ESO-1 in immunotherapy of hepatocellular carcinoma%NY-ESO-1在原发性肝癌免疫治疗中的研究进展

    Institute of Scientific and Technical Information of China (English)

    夏士珊; 向晓星

    2010-01-01

    NY-ESO-1抗原是CT抗原家族中的重要成员,在原发性肝癌组织中广泛表达,是迄今发现的最具免疫原性的肿瘤抗原,能在NY-ESO-1表达阳性的肝癌患者体内诱导自发性体液免疫反应和特异性T细胞免疫反应.因此,NY-ESO-1被认为是较好的肿瘤候选疫苗,在原发性肝癌免疫治疗中较具发展潜力.

  7. A chronic high-cholesterol diet paradoxically suppresses hepatic CYP7A1 expression in FVB/NJ mice.

    Science.gov (United States)

    Henkel, Anne S; Anderson, Kristy A; Dewey, Amanda M; Kavesh, Mark H; Green, Richard M

    2011-02-01

    Cholesterol 7α-hydroxylase (CYP7A1) encodes for the rate-limiting step in the conversion of cholesterol to bile acids in the liver. In response to acute cholesterol feeding, mice upregulate CYP7A1 via stimulation of the liver X receptor (LXR) α. However, the effect of a chronic high-cholesterol diet on hepatic CYP7A1 expression in mice is unknown. We demonstrate that chronic cholesterol feeding (0.2% or 1.25% w/w cholesterol for 12 weeks) in FVB/NJ mice results in a >60% suppression of hepatic CYP7A1 expression associated with a >2-fold increase in hepatic cholesterol content. In contrast, acute cholesterol feeding induces a >3-fold upregulation of hepatic CYP7A1 expression. We show that chronic, but not acute, cholesterol feeding increases the expression of hepatic inflammatory cytokines, tumor necrosis factor (TNF)α, and interleukin (IL)-1β, which are known to suppress hepatic CYP7A1 expression. Chronic cholesterol feeding also results in activation of the mitogen activated protein (MAP) kinases, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). Furthermore, we demonstrate in vitro that suppression of CYP7A1 by TNFα and IL-1β is dependent on JNK and ERK signaling. We conclude that chronic high-cholesterol feeding suppresses CYP7A1 expression in mice. We propose that chronic cholesterol feeding induces inflammatory cytokine activation and liver damage, which leads to suppression of CYP7A1 via activation of JNK and ERK signaling pathways.

  8. Dynamic Structure of Mo-O Species in Ag-Mo-P-O Catalyst for Oxidative Dehydrogenation of Propane

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The dynamic structure of Mo-O species in Ag-Mo-P-O catalyst was studied by in situ confocal microprobe laser Raman spectroscopy (LRS) and catalytic test. The results indicate Mo-O species of MoO3 transformed to Mo-O species of AgMoO2PO4 in C3H8/O2/N2 (3/1/4) flow at 773 K. This behavior is closely relative to oxidative dehydrogenation of propane and intrinsic properties of Mo-O species. The Mo-O species of AgMoO2PO4 may be active species for oxidative dehydrogenation of propane.

  9. Ultrafast charge transfer between MoTe2 and MoS2 monolayers

    Science.gov (United States)

    Pan, Shudi; Ceballos, Frank; Bellus, Matthew Z.; Zereshki, Peymon; Zhao, Hui

    2017-03-01

    High quality and stable electrical contact between metal and two-dimensional materials, such as transition metal dichalcogenides, is a necessary requirement that has yet to be achieved in order to successfully exploit the advantages that these materials offer to electronics and optoelectronics. MoTe2, owing to its phase changing property, can potentially offer a solution. A recent study demonstrated that metallic phase of MoTe2 connects its semiconducting phase with very low resistance. To utilize this property to connect other two-dimensional materials, it is important to achieve efficient charge transfer between MoTe2 and other semiconducting materials. Using MoS2 as an example, we report ultrafast and efficient charge transfer between MoTe2 and MoS2 monolayers. In the transient absorption measurements, an ultrashort pump pulse is used to selectively excite electrons in MoTe2. The appearance of the excited electrons in the conduction band of MoS2 is monitored by using a probe pulse that is tuned to the resonance of MoS2. We found that electrons transfer to MoS2 on a time scale of at most 0.3 ps. The transferred electrons give rise to a large transient absorption signal at both A-exciton and B-exciton resonances due to the screening effect. We also observed ultrafast transfer of holes from MoS2 to MoTe2. Our results suggest the feasibility of using MoTe2 as a bridge material to connect MoS2 and other transition metal dichalcogenides, and demonstrate a new transition metal dichalcogenide heterostructure involving MoTe2, which extends the spectral range of such structures to infrared.

  10. PEROMBAKAN SENYAWA HIDROKARBON AROMATIS POLISIKLIK (NAFTALEN PADA KADAR TINGGI OLEH Pseudomonas NY-I (Biodegradation of Polycyclic Aromatic Hydrocarbon (Naphthalene at High Concentration by Pseudomonas NY-1

    Directory of Open Access Journals (Sweden)

    Yanisworo Wijayaratih

    2001-12-01

    Full Text Available ABSTRAK Naftalen merupakan salah satu senyawa hidrokarbon aromatis polisiklik (HAP yang banyak dijumpai dalam minyak bumi, batu bara dan hasil alam lainnya. Meskipun bukan senyawa xenobiotik, naftalen dapat menjadi persoalan yang serius karena penggunaannya yang luas dan penanganan yang tidak hati-hati. Naftalen diketahui bersifat mutagenik. Penelitian ini dilakukan untuk mendapatkan isolat bakteri yang dapat merombak naftalen dan mempelajari kemampuannya merombak naftalen kadar tinggi dalam medium mineral (MM cair. Tanah yang tercemari minyak bumi dan sumber isolat diperoleh dari unit pengolah minyak Pertamina, Cilacap. Isolat dipreroleh melalui kultur diperkaya menggunakan naftalen. Jumlah naftalen yang ditambahkan ke dalam MM cair sebesar 907, 1362 dan 1813 ppm. Inkubasi dilakukan selama 28 hari dalam keadaan gelap. Parameter yang diamati meliputi: jumlah sel hidup dengan metode drop plate dan kadar naftalen sisa dengan menggunakan GC. Hasil penelitian menunjukkan bahwa isolat bakteri yang dipilih, teridentifikasi sebagai Pseudomonas NY-l. Dalam MM cair, naftalen pada semua konsentrasi terombak pada kecepatan yang mirip. Jumlah naftalen yang terombak adalah 777,3 ppm, 728,6 ppm dan 837,2 ppm dari konsentrasi awal berturut-turut sebesar 907, 1362, dan 1813 ppm.   ABSTRACT Naphthalene is one of the Polycyclic Aromatic Hydrocarbons (PAHs, found in petroleum, coal and other natural products. although, it is nonxenobiotic, it could cause a serious problem when improperly used and handled. It is considered as a mutagenic compound. This study is primarily concerned with the isolation of bacteria that could utilize naphthalene and the investigation of its biodegradation ability of naphthalene in high concentration in liquid mineral media (MM. The contaminated soil and isolates were obtained from oil treatment unit, Pertamina, Cilacap. Bacterial isolation was conducted through enriched culture. Naphthalene was added to the liquid MM at the

  11. On the Diophantine equation (12n)x+(35n)y=(37n)z%关于丢番图方程(12n)x(35n)y=(37n)z

    Institute of Scientific and Technical Information of China (English)

    杨志娟; 翁建欣

    2012-01-01

      Using the properties of congruences and the order of elements, we show that, for any positive integer n, the Diophantine equation (12n)x+(35n)y =(37n)z has no solution other than (x, y, z)=(2, 2, 2) in positive integers.%  运用同余及元素阶的性质,证明对任意正整数n,丢番图方程(12n)x+(35n)y=(37n)z仅有正整数解(x, y, z)=(2,2,2)。

  12. NY-ESO-1基因的原核表达、纯化和初步应用%The Prokaryotic Expression, Purification and Preliminary Application of Human NY-ESO-1 Gene

    Institute of Scientific and Technical Information of China (English)

    张文敏; 肖刚; 张萌; 谢丹; 郭爱林; 文剑明

    2005-01-01

    目的:克隆人NY-ESO-1基因,进行原核表达和分离纯化,并初步应用于肝癌患者血清NY-ESO-1抗体的检测.方法:通过RT-PCR技术从人肝癌组织中扩增NY-ESO-1基因片段,插入原核表达载体pGEX4T-1(+)中,构建重组表达质粒pGEX/ESO1,导入大肠杆菌BL21(DE3),诱导目的蛋白表达,经包涵体透析复性和GSTrap亲和层析纯化目的蛋白,Western blot鉴定.应用间接ELISA方法初步检测50例肝癌患者血清和20例正常人血清NY-ESO-1抗体的表达.结果:扩增得到NY-ESO-1基因3'端276bp片段,与GeneBank公布序列一致,构建的pGEX/ESO1原核表达质粒经IPTG诱导,在大肠杆菌中表达分子量约36kD的GST-ESO1融合蛋白,纯化后蛋白纯度为90%.50例肝细胞癌患者血清中4例检测到NY-ESO-1抗体(8%),正常人血清均为阴性.结论:成功构建pGEX/ESO1重组表达质粒,得到有活性的NY-ESO-1融合蛋白,对肝癌患者血清NY-ESO-1抗体的检测有一定的应用价值.

  13. Expression of NY-ESO-1 Gene in Human Bladder Cancer%NY-ESO-1基因在膀胱癌中的表达及临床意义的研究

    Institute of Scientific and Technical Information of China (English)

    王慕文; 毕东滨; 金讯波; 马庆铮

    2010-01-01

    目的 探讨NY-ESO-1的表达与膀胱癌分期分级的关系以及临床意义.方法 回顾性研究2001~2002年膀胱癌组织32例,其中男性24例,女性8例.正常膀胱粘膜对照取自耻骨上经膀胱前列腺切除术的良性前列腺增生的患者.阳性对照为正常睾丸组织.常规行RT-PCR方法检测膀胱癌组织及对照组等NY-ESO-1基因的表达,分析其表达与膀胱癌分期分级的关系以及临床意义.结果 NY-ESO-1 mRNA在32例膀胱癌组织中12例表达(37.5%),在正常膀胱粘膜上没有表达.肿瘤组织中PCR反应的产物与正常睾丸的表达产物一致.NY-ESO-1 mRNA的表达和膀胱癌的病理分级相关,有统计学差异(P<0.05).NY-ESO-1 mRNA的表达率随着肿瘤病理分级的增高而升高:G1期是0/3(0%);G2期是4/16(25%);G3期是8/13(61.54%).浸润性膀胱癌中NY-ESO-1 mRNA的表达与表浅性肿瘤的表达无显著性差异.结论 NY-ESO-1 mRNA在正常膀胱组织上没有表达,仅在正常睾丸组织和病理膀胱癌组织上有表达.NY-ESO-1 mRNA的表达率随着膀胱癌病理分级的增高而升高.

  14. Thermal infrared observations of near-Earth asteroid 2002 NY40

    CERN Document Server

    Müller, T G; Schuetz, O; Pravec, P; Siebenmorgen, R

    2004-01-01

    We obtained N-band observations of the Apollo asteroid 2002 NY40 during its close Earth fly-by in August 2002 with TIMMI2 at the ESO 3.6 m telescope. The photometric measurement allowed us to derive a radiometric diameter of 0.28+/-0.03 km and an albedo of 0.34+/-0.06 through the near-Earth asteroid thermal model (NEATM) and a thermophysical model (TPM). The values are in agreement with results from radar data, visual and near-IR observations. In this first comparison between these two model approaches we found that the empirical NEATM beaming parameter $\\eta$=1.0 corresponds to a thermal inertia values of about 100 $\\mathrm{J m^{-2} s^{-0.5} K^{-1}}$ for a typical range of surface roughness, assuming an equator-on viewing angle. Our TPM analysis indicated that the surface of 2002 NY40 consists of rocky material with a thin or no dust regolith. The asteroid very likely has a prograde sense of rotation with a cold terminator at the time of our observations. Although both model approaches can fit the thermal sp...

  15. Development of fission Mo-99 production technology

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jin Ho; Choung, W. M.; Lee, K. I. and others

    2000-05-01

    Fission Mo-99 is the only parent nuclide of Tc-99m, an extremely useful tool for mdeical diagnosis, with an estimated usage of greater than 80% of nuclear medicine applicatons. HEU and LEU targets to optimize in HANARO irradiation condition suggested and designed for domestic production of fission Mo-99. The optimum process conditions are established in each unit process to meet quality requirements of fission Mo-99 products, and the results of performance test in combined process show Mo separation and purification yield of the above 97%. The concept of Tc generator production process is established, and the result of performance test show Tc production yield of 98.4% in Tc generator procuction process. The drafts is prepared for cooperation of technical cooperation and business investment with foreign country. Evaluation on economic feasibility is accompanied for fission Mo-99 and Tc-99m generator production.

  16. Solution chemistry of Mo(III) and Mo(IV): Thermodynamic foundation for modeling localized corrosion

    Energy Technology Data Exchange (ETDEWEB)

    Wang Peiming [OLI Systems Inc., 108 American Road, Morris Plains, NJ 07950 (United States); Wilson, Leslie L.; Wesolowski, David J.; Rosenqvist, Joergen [Chemical Sciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, Oak Ridge, TN 37831-6110 (United States); Anderko, Andrzej [OLI Systems Inc., 108 American Road, Morris Plains, NJ 07950 (United States)], E-mail: aanderko@olisystems.com

    2010-05-15

    To investigate the behavior of molybdenum dissolution products in systems that approximate localized corrosion environments, solubility of Mo(III) in equilibrium with solid MoO{sub 2} has been determined at 80 deg. C as a function of solution acidity, chloride concentration and partial pressure of hydrogen. The measurements indicate a strong increase in solubility with acidity and chloride concentration and a weak effect of hydrogen partial pressure. The obtained results have been combined with literature data for systems containing Mo(III), Mo(IV), and Mo(VI) in solutions to develop a comprehensive thermodynamic model of aqueous molybdenum chemistry. The model is based on a previously developed framework for simulating the properties of electrolyte systems ranging from infinite dilution to solid saturation or fused salt limit. To reproduce the measurements, the model assumes the presence of a chloride complex of Mo(III) (i.e., MoCl{sup 2+}) and hydrolyzed species (MoOH{sup 2+}, Mo(OH){sub 2}{sup +}, and Mo(OH){sub 3}{sup 0}) in addition to the Mo{sup 3+} ion. The model generally reproduces the experimental data within experimental scattering and provides a tool for predicting the phase behavior and speciation in complex, concentrated aqueous solutions. Thus, it provides a foundation for simulating the behavior of molybdenum species in localized corrosion environments.

  17. Effects of Mo on the Microstructure and Hydrogen Sorption Properties of Ti-Mo Getters

    Institute of Scientific and Technical Information of China (English)

    ZHOU Hong-guo; WEI Xiu-ying; MAO Chang-hui; XIONG Yu-hua; QIN Guang-rong

    2007-01-01

    The effects of Mo on the microstructure evolution, porosity and hydrogen sorption properties of Ti-Mo getters are investigated in this work. The results show that the addition of Mo prolongs the densification process of Ti-Mo getters and results in a significant amount of sintered pores. With the Mo content increasing, the porosity of getters firstly increases reaching the maximum value as it attains about 7.5wt.%, and then drops. At the room temperature, the hydrogen sorption property of getters increases progressively with the Mo content increasing, but the tendency is not very clear before its content lies below 2.5wt.%. When the Mo content achieves about7.5wt.%, the hydrogen sorption property proves to be the best. The discussion is made about the above mentioned phenomena inclusive of hydrogen sorption properties of getters under different activation conditions (from 500-750 ℃).

  18. Evaluation of LAGE-1 and NY-ESO-1 expression in multiple myeloma patients to explore possible benefits of their homology for immunotherapy.

    Science.gov (United States)

    de Carvalho, Fabricio; Vettore, Andre L; Inaoka, Riguel J; Karia, Bruno; Andrade, Valéria C C; Gnjatic, Sacha; Jungbluth, Achim A; Colleoni, Gisele W B

    2011-01-20

    Due to the high homology between the LAGE-1 and NY-ESO-1 proteins, we hypothesized that an anti-NY-ESO-1 vaccine might elicit LAGE-1 immunity and hence may be effective in multiple myeloma (MM) patients with LAGE-1-positive/NY-ESO-1-negative tumors. Therefore, we set out to evaluate LAGE-1 and NY-ESO-1 mRNA and protein expression in MM patients in a bid to evaluate possible benefits of their homology for immunotherapy. LAGE-1 (a and b isoforms) and NY-ESO-1 mRNA expression was studied in 18 normal tissues and 50 bone marrow MM samples by RT-PCR. LAGE-1 and NY-ESO-1 protein expression was analyzed by immunohistochemistry (IHC) in 27 MM specimens using mAbs 219-510-23 and E978. Spontaneous serological immune response against both antigens was analyzed by ELISA in sera from 33 MM patients. LAGE-1 (a and b isoforms) was positive in 42% and NY-ESO-1 in 26% of the MM samples analyzed by RT-PCR. Both genes were found to be expressed in 18% of the cases, while at least one of the genes was found to be expressed in 50% of the cases. In LAGE-1 positive samples, 81% were positive for LAGE-1a and 19% were positive for both LAGE-1a and -1b. LAGE-1 and NY-ESO-1 protein expression could only be detected in two cases by IHC and there was a clear strong spontaneous antibody response to LAGE-1 and NY-ESO-1 in only one MM patient. In conclusion, LAGE-1a and NY-ESO-1 homology cannot be easily exploited in an anti-NY-ESO-1 vaccine given the low frequency of protein expression detected by IHC or serum analysis.

  19. Measurement of serum antibodies against NY-ESO-1 by ELISA: A guide for the treatment of specific immunotherapy for patients with advanced colorectal cancer.

    Science.gov (United States)

    Long, Yan-Yan; Wang, Yu; Huang, Qian-Rong; Zheng, Guang-Shun; Jiao, Shun-Chang

    2014-10-01

    NY-ESO-1 has been identified as one of the most immunogenic antigens; thus, is a highly attractive target for cancer immunotherapy. The present study analyzed the expression of serum antibodies (Abs) against NY-ESO-1 in patients with advanced colorectal cancer (CRC), with the aim of guiding the treatment of NY-ESO-1-based specific-immunotherapy for these patients. Furthermore, the present study was the first to evaluate the kinetic expression of anti-NY-ESO-1 Abs and investigate the possible influencing factors. A total of 239 serum samples from 155 pathologically confirmed patients with advanced CRC (stages III and IV) were collected. The presence of spontaneous Abs against NY-ESO-1 was analyzed using an enzyme-linked immunosorbent assay (ELISA). The results demonstrated that 24.5% (38/155) of the investigated patients were positive for NY-ESO-1-specific Abs. No statistically significant correlations were identified between the expression of anti-NY-ESO-1 Abs and clinicopathological parameters, including age and gender, location, grading, local infiltration, lymph node status, metastatic status and K-ras mutation status (P>0.05). In 59 patients, the kinetic expression of anti-NY-ESO-1 Abs was analyzed, of which 14 patients were initially positive and 45 patients were initially negative. Notably, 16/59 (27.1%) patients changed their expression status during the study period, and the initially positive patients were more likely to change compared with the initially negative patients (85.7 vs. 8.8%; PESO-1 by ELISA is an easy and feasible method. The high expression rate of NY-ESO-1-specific Abs in CRC patients indicates that measuring the levels of serum Abs against NY-ESO-1 may guide the treatment of NY-ESO-1-based specific immunotherapy for patients with advanced CRC.

  20. Integrated NY-ESO-1 antibody and CD8+ T-cell responses correlate with clinical benefit in advanced melanoma patients treated with ipilimumab.

    Science.gov (United States)

    Yuan, Jianda; Adamow, Matthew; Ginsberg, Brian A; Rasalan, Teresa S; Ritter, Erika; Gallardo, Humilidad F; Xu, Yinyan; Pogoriler, Evelina; Terzulli, Stephanie L; Kuk, Deborah; Panageas, Katherine S; Ritter, Gerd; Sznol, Mario; Halaban, Ruth; Jungbluth, Achim A; Allison, James P; Old, Lloyd J; Wolchok, Jedd D; Gnjatic, Sacha

    2011-10-04

    Ipilimumab, a monoclonal antibody against cytotoxic T lymphocyte antigen 4 (CTLA-4), has been shown to improve survival in patients with advanced metastatic melanoma. It also enhances immunity to NY-ESO-1, a cancer/testis antigen expressed in a subset of patients with melanoma. To characterize the association between immune response and clinical outcome, we first analyzed NY-ESO-1 serum antibody by ELISA in 144 ipilimumab-treated patients with melanoma and found 22 of 140 (16%) seropositive at baseline and 31 of 144 (22%) seropositive following treatment. These NY-ESO-1-seropositive patients had a greater likelihood of experiencing clinical benefit 24 wk after ipilimumab treatment than NY-ESO-1-seronegative patients (P = 0.02, relative risk = 1.8, two-tailed Fisher test). To understand why some patients with NY-ESO-1 antibody failed to experience clinical benefit, we analyzed NY-ESO-1-specific CD4(+) and CD8(+) T-cell responses by intracellular multicytokine staining in 20 NY-ESO-1-seropositive patients and found a surprising dissociation between NY-ESO-1 antibody and CD8 responses in some patients. NY-ESO-1-seropositive patients with associated CD8(+) T cells experienced more frequent clinical benefit (10 of 13; 77%) than those with undetectable CD8(+) T-cell response (one of seven; 14%; P = 0.02; relative risk = 5.4, two-tailed Fisher test), as well as a significant survival advantage (P = 0.01; hazard ratio = 0.2, time-dependent Cox model). Together, our data suggest that integrated NY-ESO-1 immune responses may have predictive value for ipilimumab treatment and argue for prospective studies in patients with established NY-ESO-1 immunity. The current findings provide a strong rationale for the clinical use of modulators of immunosuppression with concurrent approaches to favor tumor antigen-specific immune responses, such as vaccines or adoptive transfer, in patients with cancer.

  1. Expression of NY-ESO-1 and LAGE-1 cancer-testis antigens in hepatocellular carcinoma%NY-ESO-1和LAGE-1癌症-睾丸抗原在肝细胞癌的表达

    Institute of Scientific and Technical Information of China (English)

    张文敏; 肖刚; 张萌; 郭爱林; 董愉; 文剑明

    2005-01-01

    目的检测NY-ESO-1和LAGE-1癌症-睾丸抗原在肝细胞癌中的表达,探讨其作为肝细胞癌免疫治疗靶标的可行性及其与肝癌生物学行为的关系.方法逆转录-聚合酶链反应(RT-PCR)和免疫组织化学EnVision二步法检测30例肝细胞癌新鲜标本NY-ESO-1和LAGE-1的表达;另将191例肝癌石蜡组织制成芯片观察NY-ESO-1蛋白在肝癌中的分布和表达.结果NY-ESO-1和LAGE-1基因mRNA在肝癌中的阳性表达率分别是33.3%(10/30)和16.7%(5/30),至少表达1种基因mRNA者为36.7%(11/30);NY-ESO-1蛋白主要分布在肝癌细胞胞质,有效标本中NY-ESO-1表达13.8%(24/174),小肝癌、中晚期肝癌、发生转移肝癌中的阳性表达率逐渐升高,分别为6.8%(3/44)、16.2%(21/130)、23.1%(12/52),不发生转移的肝癌仅为9.8%(12/122),其中转移组与无转移组之间比较差异有统计学意义(P<0.05),全部病例癌组织与癌旁组织比较差异有统计学意义(P<0.01).NY-ESO-1蛋白的阳性表达与肿瘤大小无关,所有癌旁肝组织均未见NY-ESO-1和LAGE-1的mRNA和蛋白的表达.结论 NY-ESO-1/LAGE-1在肝细胞癌组织中的特异性表达提示其可作为肝细胞癌特异性免疫治疗潜在的靶标;NY-ESO-1在肝癌早期出现,随病情进展表达率逐步增高,转移患者最高,提示NY-ESO-1肽疫苗可能作为晚期肝癌患者治疗手段之一.

  2. Femtosecond mode-locked erbium-doped fiber laser based on MoS2-PVA saturable absorber

    Science.gov (United States)

    Ahmed, M. H. M.; Latiff, A. A.; Arof, H.; Ahmad, H.; Harun, S. W.

    2016-08-01

    We fabricate a free-standing few-layer molybdenum disulfide (MoS2)-polymer composite by liquid phase exfoliation of chemically pristine MoS2 crystals and use this to demonstrate a soliton mode-locked Erbium-doped fiber laser (EDFL). A stable self-started mode-locked soliton pulse is generated by fine-tuning the rotation of the polarization controller at a low threshold pump power of 25 mW. Its solitonic behavior is verified by the presence of Kelly sidebands in the output spectrum. The central wavelength, pulse width, and repetition rate of the laser are 1573.7 nm, 630 fs, and 27.1 MHz, respectively. The maximum pulse energy is 0.141 nJ with peak power of 210 W at pump power of 170 mW. This result contributes to the growing body of work studying the nonlinear optical properties of transition metal dichalcogenides that present new opportunities for ultrafast photonic applications.

  3. Resiquimod as an immunologic adjuvant for NY-ESO-1 protein vaccination in patients with high-risk melanoma.

    Science.gov (United States)

    Sabado, Rachel Lubong; Pavlick, Anna; Gnjatic, Sacha; Cruz, Crystal M; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P; Rolnitzsky, Linda; Goldberg, Judith D; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A; Bhardwaj, Nina

    2015-03-01

    The Toll-like receptor (TLR) 7/8 agonist resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide (Seppic) with or without resiquimod in patients with high-risk melanoma. In part I of the study, patients received 100 μg of full-length NY-ESO-1 protein emulsified in 1.25 mL of Montanide (day 1) followed by topical application of 1,000 mg of 0.2% resiquimod gel on days 1 and 3 (cohort 1) versus days 1, 3, and 5 (cohort 2) of a 21-day cycle. In part II, patients were randomized to receive 100-μg NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel [(arm A; n = 8) or 1,000 mg of 0.2% resiquimod gel (arm B; n = 12)] using the dosing regimen established in part I. The vaccine regimens were generally well tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4⁺ T-cell responses. CD8⁺ T-cell responses were only seen in 3 of 12 patients in arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8⁺ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical resiquimod is safe and induces both antibody and CD4⁺ T-cell responses in the majority of patients; the small proportion of CD8⁺ T-cell responses suggests that the addition of topical resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8⁺ T-cell responses.

  4. AlGaN/GaN High Electron Mobility Transistors with Multi-MgxNy/GaN Buffer

    Directory of Open Access Journals (Sweden)

    P. C. Chang

    2014-01-01

    Full Text Available We report the fabrication of AlGaN/GaN high electron mobility transistors with multi-MgxNy/GaN buffer. Compared with conventional HEMT devices with a low-temperature GaN buffer, smaller gate and source-drain leakage current could be achieved with this new buffer design. Consequently, the electron mobility was larger for the proposed device due to the reduction of defect density and the corresponding improvement of crystalline quality as result of using the multi-MgxNy/GaN buffer.

  5. Development of fission Mo-99 production technology

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jin Ho; Choung, W. M.; Lee, K. I. and others

    2001-05-01

    This R and D project is planed to supply domestic demands of Mo-99 through fission route, and consequently this project will be expected to rise up utilization of HANARO and KAERI's capability for marketing extension into domestic and oversea radiopharmaceutical market. HEU and LEU target types are decided and designed for fission Mo-99 production in domestic. Experimental study of target fabrication technology was performed and developed processing equipments. And conceptual design of target loading/unloading in/from HANARO device are performed. Tracer test of Mo-99 separation and purification process was performed, test results reach to Mo-99 recovery yield above 80% and decontamination factor above 1600. Combined Mo-99 separation and purification process was decided for hot test scheduled from next year, and performance test was performed. Conceptual design for modification of existing hot cell for fission Mo-99 production facility was performed and will be used for detail design. Assumption for the comparison of LEU and HEU target in fission Mo-99 production process were suggested and compared of merits and demerits in view of fabrication technology and economy feasibility.

  6. Antigen-specific in vitro expansion of functional redirected NY-ESO-1-specific human CD8+ T-cells in a cell-free system.

    Science.gov (United States)

    Jakka, Gopinadh; Schuberth, Petra C; Thiel, Markus; Held, Gerhard; Stenner, Frank; Van Den Broek, Maries; Renner, Christoph; Mischo, Axel; Petrausch, Ulf

    2013-10-01

    Tumors can be targeted by the adoptive transfer of chimeric antigen receptor (CAR) redirected T-cells. Antigen-specific expansion protocols are needed to generate large quantities of redirected T-cells. We aimed to establish a protocol to expand functional active NY-ESO-1-specific redirected human CD8(+) T-cells. The anti-idiotypic Fab antibody A4 with specificity for HLA-A 0201/NY-ESO-1157-165 was tested by competition assays using a HLA-A 0201/NY-ESO-1157-165 tetramer. HLA-A 0201/NY-ESO-1157-165 redirected T-cells were generated, expanded and tested for CAR expression, cytokine release, in vitro cytolysis and protection against xenografted HLA-A 0201/NY-ESO-1157-165-positive multiple myeloma cells. A4 demonstrated antigen-specific binding to HLA-A 0201/NY-ESO-1157-165 redirected T-cells. Expansion with A4 resulted in 98% of HLA-A 0201/NY-ESO-1157-165 redirected T-cells. A4 induced strong proliferation, resulting in a 300-fold increase of redirected T-cells. After expansion protocols, redirected T-cells secreted Interleukin-2, (IL-2), interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) and lysed target cells in vitro and were protective in vivo. A4 expanded HLA-A 0201/NY-ESO-1157-165 redirected T-cells with preservation of antigen-specific function.

  7. A cancer/testis antigen, NY-SAR-35, induces EpCAM, CD44, and CD133, and activates ERK in HEK293 cells.

    Science.gov (United States)

    Song, Myung-Ha; Kim, Ye-Rin; Bae, Jae-Ho; Shin, Dong-Hoon; Lee, Sang-Yull

    2017-03-04

    The cancer/testis (CT) antigen NY-SAR-35 gene is located on the X chromosome and is aberrantly expressed in various cancers but not in normal tissues, other than testes. Previously, we reported the expression of NY-SAR-35 enhanced cell growth, proliferation, and invasion in HEK293 and cancer cells. To extend understanding of the NY-SAR-35 gene, we used a next generation sequencing (NGS) approach. NY-SAR-35 expression induced growth, proliferation, metastasis, and stemness genes, as indicated by the up-regulations of CXCR4, EpCAM, CD133, and CD44, at the mRNA and protein levels. The expression of NY-SAR-35 in HEK293 cells significantly increased ERK phosphorylation, but not the phosphorylation of AKT. In HEK293/NY-SAR-35 cells, the expressions of pro-apoptotic proteins, including p53, Bax, and p21, were reduced and that of cyclin E was increased. Also, NY-SAR-35 increased the expressions of pluripotency genes (Nanog, Oct-4, and Sox2) and the ability of HEK293 cells to form colonies. Taken together, the present study indicates NY-SAR-35 functions as a CT antigen that triggers oncogenesis and self-renewal.

  8. Allorestricted T lymphocytes with a high avidity T-cell receptor towards NY-ESO-1 have potent anti-tumor activity.

    Science.gov (United States)

    Krönig, Holger; Hofer, Kathrin; Conrad, Heinke; Guilaume, Philippe; Müller, Julia; Schiemann, Matthias; Lennerz, Volker; Cosma, Antonio; Peschel, Christian; Busch, Dirk H; Romero, Pedro; Bernhard, Helga

    2009-08-01

    The cancer-testis antigen NY-ESO-1 has been targeted as a tumor-associated antigen by immunotherapeutical strategies, such as cancer vaccines. The prerequisite for a T-cell-based therapy is the induction of T cells capable of recognizing the NY-ESO-1-expressing tumor cells. In this study, we generated human T lymphocytes directed against the immunodominant NY-ESO-1(157-165) epitope known to be naturally presented with HLA-A*0201. We succeeded to isolate autorestricted and allorestricted T lymphocytes with low, intermediate or high avidity TCRs against the NY-ESO-1 peptide. The avidity of the established CTL populations correlated with their capacity of lysing HLA-A2-positive, NY-ESO-1-expressing tumor cell lines derived from different origins, e.g. melanoma and myeloma. The allorestricted NY-ESO-1-specific T lymphocytes displayed TCRs with the highest avidity and best anti-tumor recognition activity. TCRs derived from allorestricted, NY-ESO-1-specific T cells may be useful reagents for redirecting primary T cells by TCR gene transfer and, therefore, may facilitate the development of adoptive transfer regimens based on TCR-transduced T cells for the treatment of NY-ESO-1-expressing hematological malignancies and solid tumors.

  9. EPA Proposes to Add Dutchess County Creek, N.Y. to the Federal Superfund List, Sediment Contaminated with Mercury, Polycyclic Aromatic Hydrocarbons

    Science.gov (United States)

    (New York, N.Y.) The U.S. Environmental Protection Agency today proposed adding the Wappinger Creek in Dutchess County, N.Y. to its Superfund National Priorities List of the country's most hazardous waste sites. Sediment within the two mile long tidal port

  10. pCDNA3.1/NY-ESO-1真核表达质粒的构建及在肝癌细胞系HepG2中稳定高表达%To construct the eukaryotic expression plasmid pCDNA3.1/NY-ESO-1 and to establish a NY-ESO-1 highly expressed HepG2 cell line

    Institute of Scientific and Technical Information of China (English)

    徐珩; 杨硕; 顾娜; 冯丹丹; 闾军; 汪思应

    2010-01-01

    目的 建立癌-睾丸抗原NY-ESO-1稳定表达的HepG2肝癌细胞系.方法 设计NY-ESO-1的引物,PCR法从NY-ESO-1阳性表达的睾丸癌组织中扩增出目的片段,克隆至T载体,双酶切后定向连入pCDNA3.1载体,构建pCDNA3.1/NY-ESO-1真核表达质粒.经酶切、PCR、测序检测其构建的正确性,脂质体转染法将重组质粒转入HepG2细胞,G418药物筛选出稳定转染的细胞系,RT-PCR法、间接免疫荧光法分别检测稳定转染HepG2细胞中NY-ESO-1基因、蛋白的表达水平.结果 构建的pCDNA3.1/NY-ESO-1质粒经酶切、测序等检验表明质粒构建成功,筛选获得稳定高表达NY-ESO-1的HepG2细胞.结论 构建了pCDNA3.1/NY-ESO-1真核表达质粒及稳定高表达NY-ESO-1的HepG2细胞株,为下一步以NY-ESO-1为靶标进行肝癌的抗原特异性免疫治疗研究奠定了实验基础.

  11. NY-ESO-1抗原的基础与临床研究进展%Current advances in basic and clinical study of NY-ESO-1

    Institute of Scientific and Technical Information of China (English)

    虞淦军

    2015-01-01

    NY-ESO-1是癌-睾丸抗原家族中的重要一员,在多种肿瘤组织中广泛表达,但在正常组织中几乎不表达.目前已经发现了20多个抗原表位,并在多种肿瘤患者体内检测到自发性免疫反应,包括体液和细胞免疫反应,NY-ESO-1的表达及体内自发性免疫应答被证实与肿瘤的诊断和预后有重要关联.NY-ESO-1被认为是肿瘤免疫治疗的理想靶抗原,以NY-ESO-1抗原为靶点的肿瘤治疗性疫苗、TCR基因修饰的T细胞、CAR修饰的T细胞等临床研究相继开展,展示了良好的前景.

  12. From single-site tantalum complexes to nanoparticles of TaxNy and TaOxNy supported on silica: elucidation of synthesis chemistry by dynamic nuclear polarization surface enhanced NMR spectroscopy and X-ray absorption spectroscopy

    KAUST Repository

    Mohandas, Janet Chakkamadathil

    2017-06-08

    Air-stable catalysts consisting of tantalum nitride nanoparticles represented as a mixture of TaxNy and TaOxNy with diameters in the range of 0.5 to 3 nm supported on highly dehydroxylated silica were synthesized from TaMe5 (Me = methyl) and dimeric Ta-2(OMe)(10) with guidance by the principles of surface organometallic chemistry (SOMC). Characterization of the supported precursors and the supported nanoparticles formed from them was carried out by IR, NMR, UV-Vis, extended X-ray absorption fine structure, and X-ray photoelectron spectroscopies complemented with XRD and high-resolution TEM, with dynamic nuclear polarization surface enhanced NMR spectroscopy being especially helpful by providing enhanced intensities of the signals of H-1, C-13, Si-29, and N-15 at their natural abundances. The characterization data provide details of the synthesis chemistry, including evidence of (a) O-2 insertion into Ta-CH3 species on the support and (b) a binuclear to mononuclear transformation of species formed from Ta-2(OMe)(10) on the support. A catalytic test reaction, cyclooctene epoxidation, was used to probe the supported nanoparticles, with 30% H2O2 serving as the oxidant. The catalysts gave selectivities up to 98% for the epoxide at conversions as high as 99% with a 3.4 wt% loading of Ta present as TaxNy/TaOxNy.

  13. Evidence for shape coexistence in $^{98}$Mo

    CERN Document Server

    Thomas, T; Werner, V; Ahn, T; Cooper, N; Duckwitz, H; Hinton, M; Ilie, G; Jolie, J; Petkov, P; Radeck, D

    2013-01-01

    A $\\gamma\\gamma$ angular correlation experiment has been performed to investigate the low-energy states of the nucleus $^{98}$Mo. The new data, including spin assignments, multipole mixing ratios and lifetimes reveal evidence for shape coexistence and mixing in $^{98}$Mo, arising from a proton intruder configuration. This result is reproduced by a theoretical calculation within the proton-neutron interacting boson model with configuration mixing, based on microscopic energy density functional theory. The microscopic calculation indicates the importance of the proton particle-hole excitation across the Z=40 sub-shell closure and the subsequent mixing between spherical vibrational and the $\\gamma$-soft equilibrium shapes in $^{98}$Mo.

  14. 5-Aza-CdR induced expression of NY-ESO-1 antigen in tumor cells%5-Aza-CdR诱导肿瘤细胞NY-ESO-1抗原的表达

    Institute of Scientific and Technical Information of China (English)

    陈裕庆; 黄丽; 汪晓军; 张文敏

    2010-01-01

    目的:探讨甲基化抑制剂5-氮杂-2'-脱氧胞苷(5-Aza-2'-deoxycytidine,5-Aza-CdR)对肿瘤细胞中NY-ESO-1抗原表达的诱导作用.方法:常规培养人胃癌细胞株SGC-7901、人肝癌细胞株H2P和MHCC97-H、人结肠癌细胞株HT-29和LoVo及人脑胶质瘤细胞株U251,RT-PCR和免疫细胞化学法检测5-Aza-CdR作用前后肿瘤细胞中NY-ESO-1 mRNA和蛋白的表达.结果:RT-PCR与免疫细胞化学检测仅见胃癌SGC-7901细胞阳性表达NY-ESO-1,其余5株肿瘤细胞不表达NY-ESO-1.NY-ESO-1阴性的肝癌细胞株H2P、结肠癌细胞株LoVo及脑胶质瘤细胞株U251经5-Aza-CdR(终浓度分别为1、5和10 μmol/L)处理6 d后,细胞形态和生长速度均无明显改变,而NY-ESO-1 mRNA和蛋白均被诱导为阳性表达,并随5-Aza-CdR浓度增加而阳性表达逐渐增强.结论:5-Aza-CdR能诱导肝癌、结肠癌、脑胶质瘤等肿瘤细胞中NY-ESO-1抗原的表达,为肿瘤免疫治疗提供了一条新思路.

  15. A chronic high-cholesterol diet paradoxically suppresses hepatic CYP7A1 expression in FVB/NJ mice[S

    OpenAIRE

    Henkel, Anne S.; Anderson, Kristy A.; Dewey, Amanda M.; Kavesh, Mark H.; Green, Richard M.

    2011-01-01

    Cholesterol 7α-hydroxylase (CYP7A1) encodes for the rate-limiting step in the conversion of cholesterol to bile acids in the liver. In response to acute cholesterol feeding, mice upregulate CYP7A1 via stimulation of the liver X receptor (LXR) α. However, the effect of a chronic high-cholesterol diet on hepatic CYP7A1 expression in mice is unknown. We demonstrate that chronic cholesterol feeding (0.2% or 1.25% w/w cholesterol for 12 weeks) in FVB/NJ mice results in a >60% suppression of hepati...

  16. Comprehensive adipocytic and neurogenic tissue microarray analysis of NY-ESO-1 expression - a promising immunotherapy target in malignant peripheral nerve sheath tumor and liposarcoma.

    Science.gov (United States)

    Shurell, Elizabeth; Vergara-Lluri, Maria E; Li, Yunfeng; Crompton, Joseph G; Singh, Arun; Bernthal, Nicholas; Wu, Hong; Eilber, Fritz C; Dry, Sarah M

    2016-11-08

    Immunotherapy targeting cancer-testis antigen NY-ESO-1 shows promise for tumors with poor response to chemoradiation. Malignant peripheral nerve sheath tumors (MPNSTs) and liposarcomas (LPS) are chemoresistant and have few effective treatment options. Materials Methods: Using a comprehensive tissue microarray (TMA) of both benign and malignant tumors in primary, recurrent, and metastatic samples, we examined NY-ESO-1 expression in peripheral nerve sheath tumor (PNST) and adipocytic tumors. The PNST TMA included 42 MPNSTs (spontaneous n = 26, NF1-associated n = 16), 35 neurofibromas (spontaneous n = 22, NF-1 associated n = 13), 11 schwannomas, and 18 normal nerves. The LPS TMA included 48 well-differentiated/dedifferentiated (WD/DD) LPS, 13 myxoid/round cell LPS, 3 pleomorphic LPS, 8 lipomas, 1 myelolipoma, and 3 normal adipocytic tissue samples. Stained in triplicate, NY-ESO-1 intensity and density were scored. NY-ESO-1 expression was exclusive to malignant tumors. 100% of myxoid/round cell LPS demonstrated NY-ESO-1 expression, while only 6% of WD/DD LPS showed protein expression, one of which was WD LPS. Of MPNST, 4/26 (15%) spontaneous and 2/16 (12%) NF1-associated MPNSTs demonstrated NY-ESO-1 expression. Strong NY-ESO-1 expression was observed in myxoid/round cell and dedifferentiated LPS, and MPNST in primary, neoadjuvant, and metastatic settings. We found higher prevalence of NY-ESO-1 expression in MPNSTs than previously reported, highlighting a subset of MPNST patients who may benefit from immunotherapy. This study expands our understanding of NY-ESO-1 in WD/DD LPS and is the first demonstration of staining in a WD LPS and metastatic/recurrent myxoid/round cell LPS. These results suggest immunotherapy targeting NY-ESO-1 may benefit patients with aggressive tumors resistant to conventional therapy.

  17. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Science.gov (United States)

    Klar, Agnes S; Gopinadh, Jakka; Kleber, Sascha; Wadle, Andreas; Renner, Christoph

    2015-01-01

    NY-ESO-1 belongs to the cancer/testis antigen (CTA) family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC). We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165) peptide specific chimeric antigen receptor (CAR) CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels. These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  18. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Directory of Open Access Journals (Sweden)

    Agnes S Klar

    Full Text Available NY-ESO-1 belongs to the cancer/testis antigen (CTA family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC.We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165 peptide specific chimeric antigen receptor (CAR CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels.These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  19. 南极低温降解菌Shewanella sp. NJ49羟化酶分离纯化及最适产酶条件研究%Separation and purification of hydroxylase from Antarctic psychrophilic bacteriumShewanella sp. NJ49 and optimum conditions for its enzyme production

    Institute of Scientific and Technical Information of China (English)

    刘芳明; 王以斌; 曲长凤; 梁强; 郑洲; 缪锦来; 肖天

    2016-01-01

    Hydroxylase is a key enzyme with respect to bacteria during hydrocarbon degradation. In this paper, we investigate the Antarctic psychrophilic bacteriumShewanella sp. NJ49. To obtain a single component of hydroxy-lase, we separated and purified hydroxylase from NJ49 in a series of steps, i.e., ammonium sulfate precipitation, ultrafiltration concentration, and column chromatography with Sephadex G-75 as the column media. In addition, we studied the relationships between the activity of NJ49 hydroxylase and environmental factors such as temperature, pH, surfactant, and salinity. We found that purified hydroxylase comprised subunitsα,β, andγ, with estimated molecular masses of 56, 45, and 36 kD, respectively. The optimum conditions for producing NJ49 hydroxylase were a temperature of 15℃, a pH of 7.2, and a salinity of 55‰. Different types of surfactants had different effects on enzyme activity. Although individual amphoteric, cationic, and nonionic surfactants did not inhibit enzyme activity, a mixture of anionic and nonionic surfactants, as well as a mixture of cationic and nonionic surfactants, improved the activity of the hydroxylase.%羟化酶是细菌降解烷烃的关键酶,本文以南极低温降解菌Shewanella sp. NJ49为研究对象,通过硫酸铵沉淀、超滤浓缩及葡聚糖凝胶Sephadex G-75柱层析方法,分离纯化NJ49的羟化酶,获得了单一羟化酶组分,研究了温度、pH、表面活性剂和盐度等环境因素对 NJ49羟化酶酶活活性影响。结果表明,羟化酶组分由α、β、γ三个亚基组成,分子质量分别为56,45和36 kD。NJ49最适产酶条件为:温度15℃、pH7.2、盐度55;不同类型表面活性剂对酶的活性影响效应不一,两性离子表面活性剂、阳离子表面活性剂、非离子表面活性剂对酶活没有作用,阴离子表面活性剂+非离子表面活性剂混和剂和阳离子表面活性剂+非离子表面活性剂混和剂能提高羟化酶的活性。

  20. Bliv iværksætter ved at etablere en ny spin-off-virksomhed

    DEFF Research Database (Denmark)

    Clarke, Ann Højbjerg; Evald, Majbritt Rostgaard; Jensen, Kent Wickstrøm;

    2007-01-01

    eller flere iværksættere kan arbejde med spin-off-aktiviteter. Redskabet er opdelt i syv trin. Hvert trin tager fat på konkrete aktiviteter, som erhvervskonsulenten i samarbejde med en iværksætter kan gennemføre for at blive mere bevidst om, hvordan en attraktiv forretningside kan omdannes til en ny...... potentiel spin-off-virksomhed. Denne del af rapporten er oplagt at tage fat på, uanset hvor meget erfaring og viden erhvervskonsulenten har om spin-off-aktiviteter. I indholdsfortegnelsen starter denne del af rapporten med afsnittet "Arbejdsmetoden for de syv trin: Den udførlige model" og ender efter...... gennemgangen af de syv trin. Anden del i rapporten er i indholdsfortegnelsen markeret med en ramme....

  1. Manufacturing of HfOxNy films using reactive magnetron sputtering for ISFET application

    Science.gov (United States)

    Firek, Piotr; Wysokiński, Piotr

    2016-12-01

    Hafnium Oxide-Nitride films were deposited using reactive magnetron sputtering in O2/N2/Ar gas mixture. Deposition was planned according to Taguchi optimization method. Morphology of fabricated layers was tested using AFM technique (Ra=0.2÷1,0 nm). Thickness of HfOXNY films was measured using spectroscopic ellipsometry (t=45÷54 nm). Afterwards MIS structures were created by Al metallization process then layers were electrically characterised using I-V and C-V measurements. This allowed to calculate the electrical parameters of layers such as: flat-band voltage UFB, dielectric constant Ki, interface state trap density Dit and effective charge Qeff. Subsequently, deposited HfOxNy layers were annealed in PDA process (40 min 400 °C 100% N2) after which the electrical characterization was performed again.

  2. Bárány's theory is right, but incomplete. An experimental study in pigeons.

    Science.gov (United States)

    Wit, H P; Spoelstra, H A; Segenhout, J M

    1990-01-01

    The horizontal semicircular canal in pigeons was thermally stimulated with a miniature Peltier-element device. Sound evoked electric responses from the vestibular organ were modulated by the caloric stimulus. Experiments were performed under minimal influence of gravity, (horizontal canals in horizontal plane), and with the pigeons head tilted forwards or backwards. On the basis of the results it was concluded that the influence of gravity can be described by Bárány's convection theory. Extra effects are, however, present and they are most probably partly of mechanical origin (fluid expansion) and partly due to a direct influence of temperature on the vestibular receptors. Further experiments are needed to investigate the origin of these extra effects in more detail.

  3. Polar cloud observatory at Ny-Ålesund in GRENE Arctic Climate Change Research Project

    Science.gov (United States)

    Yamanouchi, Takashi; Takano, Toshiaki; Shiobara, Masataka; Okamoto, Hajime; Koike, Makoto; Ukita, Jinro

    2016-04-01

    Cloud is one of the main processes in the climate system and especially a large feed back agent for Arctic warming amplification (Yoshimori et al., 2014). From this reason, observation of polar cloud has been emphasized and 95 GHz cloud profiling radar in high precision was established at Ny-Ålesund, Svalbard in 2013 as one of the basic infrastructure in the GRENE (Green Network of Excellence Program) Arctic Climate Change Research Project. The radar, "FALCON-A", is a FM-CW (frequency modulated continuous wave) Doppler radar, developed for Arctic use by Chiba University (PI: T. Takano) in 2012, following its prototype, "FALCON-1" which was developed in 2006 (Takano et al., 2010). The specifications of the radar are, central frequency: 94.84 GHz; antenna power: 1 W; observation height: up to 15 km; range resolution: 48 m; beam width: 0.2 degree (15 m at 5 km); Doppler width: 3.2 m/s; time interval: 10 sec, and capable of archiving high sensitivity and high spatial and time resolution. An FM-CW type radar realizes similar sensitivity with much smaller parabolic antennas separated 1.4 m from each other used for transmitting and receiving the wave. Polarized Micro-Pulse Lidar (PMPL, Sigma Space MPL-4B-IDS), which is capable to measure the backscatter and depolarization ratio, has also been deployed to Ny-Ålesund in March 2012, and now operated to perform collocated measurements with FALCON-A. Simultaneous measurement data from collocated PMPL and FALCON-A are available for synergetic analyses of cloud microphysics. Cloud mycrophysics, such as effective radius of ice particles and ice water content, are obtained from the analysis based on algorithm, which is modified for ground-based measurements from Okamoto's retrieval algorithm for satellite based cloud profiling radar and lidar (CloudSat and CALIPSO; Okamoto et al., 2010). Results of two years will be shown in the presentation. Calibration is a point to derive radar reflectivity (dBZ) from original intensity data

  4. Observations of stratospheric ozone above Ny-Ålesund in the Arctic, 2010-2011

    Institute of Scientific and Technical Information of China (English)

    LUO Yuhan; SI Fuqi; LIU Wenqing; SUN Liguang; LIU Yi

    2015-01-01

    Stratosphere ozone depletion above the Arctic region has drawn increased attention recently. Here we present stratospheric ozone column densities above Ny-Ålesund in the Arctic during summer 2010 and 2011, based on a self-developed passive differential optical absorption spectroscopy (DOAS) technique. By analyzing the received scattered solar spectrum, daily variations of ozone vertical column densities (VCDs) were obtained and correlated with satellite-borne ozone monitoring results and ozone sonde data. The comparisons showed good correlation, conifrming the feasibility of DOAS in high-latitude Arctic regions. The preliminary analysis also demonstrated that abnormal low-level ozone columns found in spring 2011 had negative impacts on total ozone column densities over the entire year. The loss of stratospheric ozone may be correlated with low stratospheric temperatures, where heterogeneous atmospheric reactions were active.

  5. The first observation of Mo5+ in the passivation layer of Mo2N

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Molybdenum nitride powder with sg of 115 m2·g-1 (passivated) has been prepared by a temperature programmed reaction of MoO3 in H2/N2 mixture. It exhibited high catalytic activity in CO oxidation at low temperature. XPS, EPR and LRS studies have shown the existence of mixed valence states of Mo ions, especially Mo5+ ion (g⊥=1.932, g‖=1.892) observed for the first time, in the passivation layer of molybdenum nitride. A surface superoxide species, O-2(g = 2.001, Raman band 1 124 cm-1), was found to be produced accompanying the transformation of Mo5+/Mo4+ redox pair. Evidence has been given to suggest that this surface superoxide might be responsible for CO oxidation over Mo2N catalyst.

  6. QUEST-lærere fra Holstebro får ny naturfags”trailer” i spil

    DEFF Research Database (Denmark)

    Lysgaard, Troels; Sørensen, Allan; Nielsen, Birgitte Lund;

    2015-01-01

    Ungdomsskolen i Holstebro fik mulighed for at udstyre en ny naturfagstrailer til brug for kommunens naturfagslærere. I den forbindelse var det oplagt at anvende netværket fra to år i QUEST projektet. Derfor har naturfagslærere på efteruddannelse i QUEST målsat, designet og afprøvet undervisningsi...

  7. En ny historie om guld og svovl - guld-svovlbindingen i thiolers adsorption på guldnanopartikler og plane guldoverflader

    DEFF Research Database (Denmark)

    Chi, Qijin; Ford, Michael J.; Halder, Arnab;

    2016-01-01

    En netop publiceret større australsk-dansk undersøgelse giver ny indsigt i, hvordan thioldækkede Au-overflader er opbygget. Det har betydning for design, syntese og dannelsesmekanismer for Au-nanopartikler og Au-overflader med skræddersyede fysiske og kemiske egenskaber....

  8. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Black Rock Canal and Lock at... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal” when used in this section will mean all of the Black Rock Waterway, including Black Rock Lock, and...

  9. Nano-Crystallization of High-Entropy Amorphous NbTiAlSiWxNy Films Prepared by Magnetron Sputtering

    Directory of Open Access Journals (Sweden)

    Wenjie Sheng

    2016-06-01

    Full Text Available High-entropy amorphous NbTiAlSiWxNy films (x = 0 or 1, i.e., NbTiAlSiNy and NbTiAlSiWNy were prepared by magnetron sputtering method in the atmosphere of a mixture of N2 + Ar (N2 + Ar = 24 standard cubic centimeter per minute (sccm, where N2 = 0, 4, and 8 sccm. All the as-deposited films present amorphous structures, which remain stable at 700 °C for over 24 h. After heat treatment at 1000 °C the films began to crystalize, and while the NbTiAlSiNy films (N2 = 4, 8 sccm exhibit a face-centered cubic (FCC structure, the NbTiAlSiW metallic films show a body-centered cubic (BCC structure and then transit into a FCC structure composed of nanoscaled particles with increasing nitrogen flow rate. The hardness and modulus of the as-deposited NbTiAlSiNy films reach maximum values of 20.5 GPa and 206.8 GPa, respectively. For the as-deposited NbTiAlSiWNy films, both modulus and hardness increased to maximum values of 13.6 GPa and 154.4 GPa, respectively, and then decrease as the N2 flow rate is increased. Both films could be potential candidates for protective coatings at high temperature.

  10. MAGE-A1, GAGE and NY-ESO-1 cancer/testis antigen expression during human gonadal development

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Kock, Kirsten; Nielsen, Ole;

    2007-01-01

    by single and double immunohistochemical staining. RESULTS: We found that GAGE was expressed in the primordial germ cells of the gonadal primordium, whereas MAGE-A1 and NY-ESO-1 were first detected in germ cells of both testis and ovary after sexual differentiation was initiated. The number of positive germ...

  11. Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

    DEFF Research Database (Denmark)

    Andersen, Lars Dyrskjøt; Zieger, K; Kissow Lildal, T;

    2012-01-01

    Background: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. Methods: We used specific q-RT-PCR assays to analyse...... the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. Results: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed...... PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P=0.026), LAGE-1 (P=0.001) and NY-ESO-1 (P=0.040) was significantly associated with a shorter...

  12. Development and Initial Validation of the NyTid Test: A Movement Assessment Tool for Compulsory School Pupils

    Science.gov (United States)

    Tidén, Anna; Lundqvist, Carolina; Nyberg, Marie

    2015-01-01

    This study presents the development process and initial validation of the NyTid test, a process-oriented movement assessment tool for compulsory school pupils. A sample of 1,260 (627 girls and 633 boys; mean age of 14.39) Swedish school children participated in the study. In the first step, exploratory factor analyses (EFAs) were performed in…

  13. MAGE-A is More Highly Expressed Than NY-ESO-1 in a Systematic Immunohistochemical Analysis of 3668 Cases.

    Science.gov (United States)

    Kerkar, Sid P; Wang, Zeng-Feng; Lasota, Jerzy; Park, Tristen; Patel, Krishna; Groh, Eric; Rosenberg, Steven A; Miettinen, Markku M

    2016-05-01

    Two cancer testis antigens, the New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and the melanoma-antigen family A (MAGE-A), represent promising immunotherapy targets due to the low expression of these antigens in nonmalignant tissue. To assess overexpression patterns in various cancers, we performed a systematic immunohistochemical analysis for NY-ESO-1 and MAGE-A on tissue array samples of 3668 common epithelial carcinomas (CA) and germ cell tumors of high prevalence and mortality. Here, we find significantly higher expression of MAGE-A (>50% on tumor cells) compared with NY-ESO-1 in several CAs including cutaneous squamous cell carcinomas (SCC) (52.8%/2.8%), esophageal SCC (50%/0%), head and neck SCC (41.1%/ESO-1, was seen in whole slide evaluations of an independent cohort of metastatic SCC (45.5%/3.6%) and metastatic CA (13.5%/0%) of various primaries with significantly higher expression of MAGE-A in metastatic SCC compared with other metastatic CA. MAGE-A is also more highly expressed in germ cell tumors, seminomas (69%/3.5%) and nonseminomas (40.1%/4.7%). In summary, MAGE-A is more highly expressed than NY-ESO-1 in a majority of human malignancies, and targeting MAGE-A may benefit a large number of patients.

  14. Implementering af ny standard for kateterisering i forhold til ældre svagelige patienter > 65 år med hoftefraktur

    DEFF Research Database (Denmark)

    Bagger, Bettan; Varmarken, Tine; Pedersen, Niels

    2013-01-01

    klinisk standard. Metode: Der er udarbejdet et systematisk review med henblik på at identificere best evidence i forhold til brug af intermitterende versus permanent kateterisation. Der er implementeret en ny evidensbaseret standard i afdelingen, som evaluereres gennem en efterfølgende audit og...

  15. 76 FR 66685 - Foreign-Trade Zone 37-Orange County, NY, Application for Subzone, ITT Water Technology, Inc...

    Science.gov (United States)

    2011-10-27

    ... Technology, Inc. (Centrifugal and Submersible Pumps), Auburn, NY An application has been submitted to the... special purpose subzone status for the centrifugal and submersible pump manufacturing and warehousing... facilities are used to manufacture and distribute centrifugal and submersible pumps and related...

  16. 33 CFR 100.121 - Swim Across the Sound, Long Island Sound, Port Jefferson, NY to Captain's Cove Seaport...

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Swim Across the Sound, Long... SAFETY OF LIFE ON NAVIGABLE WATERS § 100.121 Swim Across the Sound, Long Island Sound, Port Jefferson, NY... they are officially participating in the Swim Across the Sound event or are otherwise authorized by...

  17. 77 FR 46377 - Foreign-Trade Zone 37-Orange County, NY, Notification of Proposed Production Activity, Takasago...

    Science.gov (United States)

    2012-08-03

    ..., mixtures of odiferous substances and essential oils (duty rate ranges from duty free to 7%). Public comment... Foreign-Trade Zones Board Foreign-Trade Zone 37--Orange County, NY, Notification of Proposed Production... (Takasago) submitted a notification of proposed production activity for their facility located in...

  18. Floodplain Mapping Submission for Oregon County, MO

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping study deliverables depict and quantify the flood risks for Oregon County, MO. The City of Thayer and the Missouri State Emergency Management...

  19. 78 FR 45283 - Missouri Disaster #MO-00066

    Science.gov (United States)

    2013-07-26

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION Missouri Disaster MO-00066 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... adversely affected by the disaster: Primary Counties: Barton; Callaway; Cape Girardeau; Chariton;...

  20. Phase transformations in Mo-doped FINEMETs

    Energy Technology Data Exchange (ETDEWEB)

    Silveyra, Josefina M., E-mail: jsilveyra@fi.uba.a [Lab. de Solidos Amorfos, INTECIN, FIUBA-CONICET, Paseo Colon 850, (C1063ACV) Buenos Aires (Argentina); Illekova, Emilia; Svec, Peter; Janickovic, Dusan [Institute of Physics SAS, Dubravska cesta 9, 845 11 Bratislava (Slovakia); Rosales-Rivera, Andres [Laboratorio de Magnetismo y Materiales Avanzados, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Colombia, Manizales (Colombia); Cremaschi, Victoria J. [Lab. de Solidos Amorfos, INTECIN, FIUBA-CONICET, Paseo Colon 850, (C1063ACV) Buenos Aires (Argentina)

    2010-06-15

    In this paper, the phase transformations occurring during the crystallization process of FINEMETs in which Nb has been gradually replaced by Mo have been studied by a variety of techniques including DSC, DTA, TGA, XRD and TEM. The thermal stability of the alloy was deteriorated as a consequence of Mo's smaller atomic size. The gradual replacement of Nb by Mo reduced the onset temperature of Fe-Si and of the borides. The Curie temperature of the amorphous phase slightly decreased from 594 K for x=0 to 587 K for x=3. The borides compounds Fe{sub 2}B and Fe{sub 23}B{sub 6} as well as the (Nb,Mo){sub 5}Si{sub 3} phase were found to precipitate in the second and third crystallization.

  1. Tl2Mo9Se11

    Directory of Open Access Journals (Sweden)

    Patrick Gougeon

    2010-08-01

    Full Text Available The structure of Tl2Mo9Se11, dithallium nonamolybdenum undecaselenide, is isotypic with Tl2Mo9S11 [Potel et al. (1980. Acta Cryst. B36, 1319–1322]. The structural set-up is characterized by a mixture of Mo6Sei8Sea6 and Mo12Sei14Sea6 cluster units in a 1:1 ratio. Both components are interconnected through interunit Mo—Se bonds. The cluster units are centered at Wyckoff positions 3a and 3b (point-group symmetry overline{3}.. The two TlI atoms are situated in the voids of the three-dimensional arrangement. Two of the five independent Se atoms and the Tl atoms lie on sites with 3. symmetry (Wyckoff site 6c.

  2. Cancer-testis antigens PRAME and NY-ESO-1 correlate with tumour grade and poor prognosis in myxoid liposarcoma.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Hotokebuchi, Yuka; Ishii, Takeaki; Maekawa, Akira; Yamada, Yuichi; Yamamoto, Hidetaka; Iwamoto, Yukihide; Oda, Yoshinao

    2015-07-01

    Myxoid liposarcoma is the second most common liposarcoma. Although myxoid liposarcoma is relatively chemosensitive and thus a good candidate for chemotherapy, cases with relapsed or metastatic disease still have poor outcome. Here, we performed a gene microarray analysis to compare the gene expression profiles in six clinical myxoid liposarcoma samples and three normal adipose tissue samples, and to identify molecular biomarkers that would be useful as diagnostic markers or treatment targets in myxoid liposarcoma. This showed that the cancer-testis antigen PRAME was up-regulated in myxoid liposarcoma. We then performed immunohistochemical, western blotting and real-time polymerase chain reaction analyses to quantify the expression of PRAME and another cancer-testis antigen, NY-ESO-1, in clinical samples of myxoid liposarcoma (n = 93), dedifferentiated (n = 46), well-differentiated (n = 32) and pleomorphic liposarcomas (n = 14). Immunohistochemically, positivity for PRAME and NY-ESO-1 was observed in 84/93 (90%) and 83/93 (89%) of the myxoid liposarcomas, and in 20/46 (43%) and 3/46 (7%) of the dedifferentiated, 3/32 (9%) and 1/32 (3%) of the well-differentiated and 7/14 (50%) and 3/21 (21%) of the pleomorphic liposarcomas, respectively. High immunohistochemical expression of PRAME and/or NY-ESO-1 was significantly correlated with tumour diameter, the existence of tumour necrosis, a round-cell component of >5%, higher histological grade and advanced clinical stage. High PRAME and NY-ESO-1 expression correlated significantly with poor prognosis in a univariate analysis. The myxoid liposarcomas showed significantly higher protein and mRNA expression levels of PRAME and NY-ESO-1 (CTAG1B) than the other liposarcomas. In conclusion, PRAME and NY-ESO-1 (CTAG1B) were expressed in the vast majority of myxoid liposarcomas, and their high-level expression correlated with tumour grade and poor prognosis. Our results support the potential use of PRAME and NY

  3. Reaction mechanism of core-shell MoO2/MoS2 nanoflakes via plasma-assisted sulfurization of MoO3

    Science.gov (United States)

    Kumar, Prabhat; Singh, Megha; Sharma, Rabindar K.; Reddy, G. B.

    2016-05-01

    The sulfurization of MoO3 in an H2S/Ar plasma atmosphere has been experimentally studied and a reaction mechanism has been proposed based on the results obtained. Nanostructured thin films (NTFs) of MoO3 were sulfurized at different temperatures varying from 150 °C to 550 °C. High-resolution transmission electron microscopy (TEM) images depict core-shell nanoflakes with varying shell thicknesses as the sulfurization temperature (T sn) is varied. The shells consist of MoS2 and the core is MoO2/MoO3. X-ray diffraction (XRD) and Raman analysis have been used to study the structural changes as MoO3 is sulfurized. The analyses showed two phases, MoO2 and MoS2, at low temperatures (≤350 °C), whereas the films sulfurized at higher temperatures show predominantly MoS2. The scanning electron microscopy (SEM) results show no noticeable changes in the surface morphology of the NTFs after sulfurization. X-ray photoelectron spectroscopy (XPS) was carried out to calculate the relative percentages of MoO3, MoO2 and MoS2. It is revealed that sulfurization of MoO3 in the plasma is affected by T sn. The sulfurization process occurs in two steps, involving the reduction of MoO3 to form MoO2 in the first step, followed by MoO2 being converted into MoS2. It is also evident that the reduction of MoO3 is more a result of the reactive species of hydrogen (H*) than the replacement of oxygen by sulfur in the second step.

  4. Immunoediting and persistence of antigen-specific immunity in patients who have previously been vaccinated with NY-ESO-1 protein formulated in ISCOMATRIX™.

    Science.gov (United States)

    Nicholaou, Theo; Chen, Weisan; Davis, Ian D; Jackson, Heather M; Dimopoulos, Nektaria; Barrow, Catherine; Browning, Judy; Macgregor, Duncan; Williams, David; Hopkins, Wendie; Maraskovsky, Eugene; Venhaus, Ralph; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Cebon, Jonathan

    2011-11-01

    NY-ESO-1 protein formulated in ISCOMATRIX™ results in CD4+, CD8+ T cell and antibody-mediated immunity. We evaluated persistence of immunity, relapse-free survival and tumour antigen expression upon relapse in patients vaccinated in an earlier trial. Immunity was measured in 28 patients with resected NY-ESO-1-expressing tumours (melanoma 25, breast 3) 252-1,155 days (median = 681) after vaccination. In the earlier vaccination, trial patients received NY-ESO-1 with ISCOMATRIX™ adjuvant at three protein doses 10 μg, 30 μg or 100 μg (n = 14); 100 μg NY-ESO-1 protein (n = 8) or placebo (n = 6), together with 1 μg of intradermal (ID) NY-ESO-1 protein twice for DTH skin testing. Immune responses assessed in the current study included antibody titres, circulating NY-ESO-1-specific T cells and DTH reactivity 2 days after DTH skin testing with NY-ESO-1 protein (1 μg) or peptides (10 μg). Relapse-free survival was determined for 42 melanoma patients. On relapse NY-ESO-1 and HLA, class I was assessed by immunohistochemistry in 17. Persisting anti-NY-ESO-1 immunity was detected in 10/14 recipients who had previously received vaccine with ISCOMATRIX™ adjuvant. In contrast, immunity only persisted in 3/14 who received 100 μg un-adjuvanted NY-ESO-1 protein (3/8) or 2 μg DTH protein (0/6) P = 0.02. Hence, persisting NY-ESO-1 immunity was associated with prior adjuvant. Tumour NY-ESO-1 or HLA class I was downregulated in participants who relapsed suggesting immunoediting had occurred. Immunoediting suggests that a signal of anti-tumour activity was observed in high-risk resected melanoma patients vaccinated with NY-ESO-1/ISCOMATRIX™. This was associated with measurable persisting immunity in the majority of vaccinated subjects tested. A prospective randomised trial has been undertaken to confirm these results.

  5. Mo100 to Mo99 Target Cooling Enhancements Report

    Energy Technology Data Exchange (ETDEWEB)

    Woloshun, Keith Albert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Dale, Gregory E. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Olivas, Eric Richard [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Mocko, Michal [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-02-16

    Target design requirements changed significantly over the past year to a much higher beam current on larger diameter disks, and with a beam impingement on both ends of the target. Scaling from the previous design, that required significantly more mass flow rate of helium coolant, and also thinner disks. A new Aerzen GM12.4 blower was selected that can deliver up to 400 g/s at 400 psi, compared to about 100 g/s possible with the Tuthill blower previously selected.Further, to accommodate the 42 MeV, 2.7 mA beam on each side of the target, the disk thickness and the coolant gaps were halved to create the current baseline design: 0.5 mm disk thickness (at 29 mm diameter) and 0.25 mm coolant gap. Thermal-hydraulic analysis of this target, presented below for reference, gave very good results, suggesting that the target could be improved with fewer, thicker disks and with disk thickness increasing toward the target center. The total thickness of Mo100 in the target remaining the same, that reduces the number of coolant gaps. This allows for the gap width to be increased, increasing the mass flow in each gap and consequently increasing heat transfer. A preliminary geometry was selected and analyzed with variable disk thickness and wider coolant gaps. The result of analysis of this target shows that disk thickness increase near the window was too aggressive and further resizing of the disks is necessary, but it does illustrate the potential improvements that are possible. Experimental and analytical study of diffusers on the target exit has been done. This shows modest improvement in requcing pressure drop, as will be summarized below. However, the benefit is not significant, and implementation becomes problematic when disk thickness is varying. A bull nose at the entrance does offer significant benefit and is relatively easy to incorporate. A bull nose on both ends is now a feature of the baseline design, and will be a feature of any redesign or enhanced designs that

  6. Synthesis of MoSi2 by Mechanical Alloying

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The microstructure evolution of mechanical alloyed Mo-66.7%Si powder using the high-energy ball mill has been studied by X-ray diffraction and scanning electron microscopy. The results showed that MoSi2 can be synthesized by MA of Mo-66.7%Si powder mixtures. Cold welding behavior between Mo and Si powders plays an important role in the preparation of MoSi2 by the MA.

  7. Fine-mapping naturally occurring NY-ESO-1 antibody epitopes in melanoma patients' sera using short overlapping peptides and full-length recombinant protein.

    Science.gov (United States)

    Komatsu, Nobukazu; Jackson, Heather M; Chan, Kok-fei; Oveissi, Sara; Cebon, Jonathan; Itoh, Kyogo; Chen, Weisan

    2013-07-01

    The tumor antigen NY-ESO-1 is one of the most antigenic cancer-testis antigens, first identified by serologic analysis of a recombinant cDNA expression library (SEREX). NY-ESO-1 is expressed in different types of cancers including melanoma. NY-ESO-1-specific spontaneous humoral and cellular immune responses are detected in a large proportion of patients with advanced NY-ESO-1-expressing cancers. Therefore NY-ESO-1 is a good candidate antigen for immunotherapy. Although cellular immune responses to NY-ESO-1 are well characterized, much less is known about the humoral immune responses. In this study, we finely mapped linear antibody epitopes using sera from melanoma patients and shorter overlapping peptide sets. We have shown that melanoma patients' humoral immune systems responded to NY-ESO-1 differently in each individual with widely differing antibody specificity, intensity and antibody subtypes. This knowledge will help us further understand anti-tumor immunity and may also help us to monitor cancer progress and cancer vaccine efficacy in the future. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Expression of NY-ESO-1 in Triple-Negative Breast Cancer Is Associated with Tumor-Infiltrating Lymphocytes and a Good Prognosis.

    Science.gov (United States)

    Lee, Hee Jin; Kim, Joo Young; Song, In Hye; Park, In Ah; Yu, Jong Han; Gong, Gyungyub

    2015-01-01

    Accumulating evidence suggests that immunotherapy has great potential for treating triple-negative breast cancer (TNBC). We analyzed the expression of NY-ESO-1, which is a potent immunogenic cancer testis antigen, and its association with clinicopathological factors in large cohorts of breast cancer patients. A total of 623 consecutive breast cancer patients who underwent surgery between 1993 and 1998 and 612 TNBC patients who underwent surgery between 2004 and 2010 at Asan Medical Center were included. Immunohistochemical staining for NY-ESO-1 was performed using tissue microarrays. NY-ESO-1 was expressed in 2.6% of consecutive breast cancers, all of which were TNBC (p ESO-1 expression was identified in 9.7% of the TNBC cohort and was significantly correlated with a higher level of tumor-infiltrating lymphocytes (TIL; p = 0.026). In survival analyses, a lower level of TIL (all, p ESO-1 expression (p = 0.024) were significantly associated with poor disease-free survival. Additionally, positive NY-ESO-1 expression was an independent favorable prognostic factor in TNBC patients (p = 0.046). NY-ESO-1 is specifically expressed in TNBC, and NY-ESO-1 expression is an independent good prognostic factor in TNBC. Evaluation of NY-ESO-1 expression in TNBC might be useful for selecting patients who may benefit from vaccination therapy and also has a prognostic significance in TNBC. © 2015 S. Karger AG, Basel.

  9. Persistent deNOx Ability of CaAl2O4:(Eu, Nd/TiO2-xNy Luminescent Photocatalyst

    Directory of Open Access Journals (Sweden)

    Li Huihui

    2011-01-01

    Full Text Available Abstract CaAl2O4:(Eu, Nd/TiO2-xNy composite luminescent photocatalyst was successfully synthesized by a simple planetary ball milling process. Improvement of photocatalytic deNOx ability of TiO2-xNy, together with the persistent photocatalytic activity for the decomposition of NO after turning off the light were realized, by coupling TiO2-xNy with long afterglow phosphor, CaAl2O4:(Eu, Nd. The novel persistent photocatalytic behavior was related to the overlap between the absorption wavelength of TiO2-xNy and the emission wavelength of the CaAl2O4:(Eu, Nd. It was found that CaAl2O4:(Eu, Nd/TiO2-xNy composites provided the luminescence to persist photocatalytic reaction for more than 3 h after turning off the light. Graphical Abstract CaAl2O4:(Eu, Nd/TiO2-xNy composite luminescent photocatalyst with persistent deNOx activity after turning off the light was successfully synthesized by a simple planetary ball milling process. The novel persistent photocatalytic behavior was related to the overlap between the absorption wavelength of TiO2-xNy and the emission wavelength of the CaAl2O4:(Eu, Nd. Additional file 1 Click here for file

  10. Impact of reduced graphene oxide on MoS{sub 2} grown by sulfurization of sputtered MoO{sub 3} and Mo precursor films

    Energy Technology Data Exchange (ETDEWEB)

    Pacley, Shanee, E-mail: shanee.pacley@us.af.mil; Brausch, Jacob; Beck-Millerton, Emory [U.S. Air Force Research Laboratory (AFRL)/Wright Patterson Air Force Base, Wright Patterson, Ohio 45433-7707 (United States); Hu, Jianjun; Jespersen, Michael [University of Dayton Research Institute, 300 College Park, Dayton, Ohio 45469 (United States); Hilton, Al [Wyle Laboratories, 4200 Colonel Glenn Hwy, Beavercreek, Ohio 45431 (United States); Waite, Adam [University Technology Corporation, 1270 N Fairfield Rd., Beavercreek, Ohio 45432 (United States); Voevodin, Andrey A. [Department of Materials Science and Engineering, University of North Texas, 1155 Union Circle, Denton, Texas 76203 (United States)

    2016-07-15

    Monolayer molybdenum disulfide (MoS{sub 2}), a two dimensional semiconducting dichalcogenide material with a bandgap of 1.8–1.9 eV, has demonstrated promise for future use in field effect transistors and optoelectronics. Various approaches have been used for MoS{sub 2} processing, the most common being chemical vapor deposition. During chemical vapor deposition, precursors such as Mo, MoO{sub 3}, and MoCl{sub 5} have been used to form a vapor reaction with sulfur, resulting in thin films of MoS{sub 2}. Currently, MoO{sub 3} ribbons and powder, and MoCl{sub 5} powder have been used. However, the use of ribbons and powder makes it difficult to grow large area-continuous films. Sputtering of Mo is an approach that has demonstrated continuous MoS{sub 2} film growth. In this paper, the authors compare the structural properties of MoS{sub 2} grown by sulfurization of pulse vapor deposited MoO{sub 3} and Mo precursor films. In addition, they have studied the effects that reduced graphene oxide (rGO) has on MoS{sub 2} structure. Reports show that rGO increases MoS{sub 2} grain growth during powder vaporization. Herein, the authors report a grain size increase for MoS{sub 2} when rGO was used during sulfurization of both sputtered Mo and MoO{sub 3} precursors. In addition, our transmission electron microscopy results show a more uniform and continuous film growth for the MoS{sub 2} films produced from Mo when compared to the films produced from MoO{sub 3}. Atomic force microscopy images further confirm this uniform and continuous film growth when Mo precursor was used. Finally, x-ray photoelectron spectroscopy results show that the MoS{sub 2} films produced using both precursors were stoichiometric and had about 7–8 layers in thickness, and that there was a slight improvement in stoichiometry when rGO was used.

  11. Production of 99Mo Using 33 MeV Electron and 100Mo Target

    Institute of Scientific and Technical Information of China (English)

    LIU; Bao-jie; LI; Jin-hai; ZENG; Zi-qiang

    2015-01-01

    The(γ,n)reaction on a 100Mo target is considered to produce the radioactive isotope 100Mo.The advantages of this reaction are the relatively high reaction cross section and the elimination of the expensive fission products chemistry steam associated with irradiation of uranium.The rela-

  12. EFFECT OF CONCENTRATION METAL PRECURSOR Co AND Mo ON CHARACTER OF CoMo / USY CATALYST

    Directory of Open Access Journals (Sweden)

    Khoirina Dwi Nugrahaningtyas

    2016-08-01

    Full Text Available The preparation and characterization of bimetallic catalysts using impregnation method with a variation of concentration of precursor sequence Co and Mo metal obtained catalyst K 1 [Co (0.018 M - Mo (0.037 M/USY]. K 2 [Co (0.026 M - Mo (0.055 M/USY], K 3 [Co (0.035 M - Mo (0.074 M/USY], K 4 [Co (0.05 M - Mo (0.11 M /USY] and K 5 [Co (0.107 M - Mo (0.22 M/USY]. Character of the catalyst in terms of crystallinity was analyzed by XRD. The result shows that there is no cristalinity damage of USY after impregnation but the amorphous cristalin structure was obtained. Amount of metal content was analyzed by XRF and the catalyst morphology by SEM-EDS. The result shows that the higher the concentration of Co and Mo so that find the higher content of metal in catalyst of the prepared catalyst increase. K 4 shows the best characteristic of catalysts prepared in this research. Analysis of K 4 is proving that Co and Mo are presented in catalyst.

  13. Study of tribological behavior of Cu–MoS2 and Ag–MoS2 nanocomposite lubricants

    OpenAIRE

    An, V.; Anisimov, E.; Druzyanova, V.; Burtsev, N.; Shulepov, I.; Khaskelberg, M.

    2016-01-01

    Tribological behavior of Cu–MoS2 and Ag–MoS2 nanocomposite lubricant was studied. Cu nanoparticles produced by electrical explosion of copper wires and Ag nanoparticles prepared by electrospark erosion were employed as metal cladding modifiers of MoS2 nanolamellar particles. The tribological tests showed Cu–MoS2 and Ag–MoS2 nanocomposite lubricants changed the friction coefficient of the initial grease and essentially improved its wear resistance.

  14. States of carbon nanotube supported Mo-based HDS catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Shang, Hongyan; Liu, Chenguang; Xu, Yongqiang [Key Laboratory of Catalysis, CNPC, College of Chemistry and Chemical Engineering, University of Petroleum, Dongying 257061 (China); Qiu, Jieshan [Carbon Research Laboratory, Center for Nano Materials and Science, Dalian University of Technology, 158 Zhongshan Road, P. O. Box 49, Dalian 116012 (China); Wei, Fei [Department of Chemical Engineering, Tsinghua University, Being, 100084 (China)

    2007-02-15

    As HDS catalysts, the supported catalysts including oxide state Mo, Co-Mo and sulfide state Mo on carbon nanotube (CNT) were prepared, while the corresponding supported catalysts on {gamma}-Al{sub 2}O{sub 3} were prepared as comparison. Firstly, the dispersion of the active phase and loading capacity of Mo species on CNT was studied by XRD and the reducibility properties of Co-Mo catalysts in oxide state over CNTs were investigated by TPR while the sulfide Co-Mo/CNT catalysts were characterized by XRD and LRS techniques. Secondly, the activity and selectivity of hydrodesulfurization (HDS) of dibenzothiophene with Co-Mo/CNT and Co-Mo/{gamma}-Al{sub 2}O{sub 3} were studied. It has been found that the main active molybdenum species in the oxide state MoO{sub 3}/CNT catalysts were MoO{sub 2}, rather than MoO{sub 3} as generally expected. The maximum loading before formation of the bulk phase was lower than 6%m (calculated in MoO{sub 3}). The TPR studies revealed that that active species in oxide state Co-Mo/CNT catalysts were more easily reduced at relatively lower temperatures in comparison to those in Co-Mo/{gamma}-Al{sub 2}O{sub 3}, indicating that the CNT support promoted the reduction of active species. Among 0-1.0 Co/Mo atomic ratio on Co-Mo/CNT, 0.7 has the highest reducibility. It shows that the Co/Mo atomic ratio has a great effect on the reducibility of active species on CNT and their HDS activities and that the incorporation of cobalt improved the dispersion of molybdenum species on CNT and mobilization. It was also found that re-dispersion could occur during the sulfiding process, resulting in low valence state Mo{sub 3}S{sub 4} and Co-MoS{sub 2.17} active phases. The HDS of DBT showed that Co-Mo/CNT catalysts were more active than Co-Mo/{gamma}-Al{sub 2}O{sub 3} and the hydrogenolysis/hydrogenation selectivity of Co-Mo/CNT catalyst was also much higher than Co-Mo/{gamma}-Al{sub 2}O{sub 3}. For the Co-Mo/CNT catalysis system, the catalyst with Co/Mo atomic

  15. The cancer-testis antigen NY-ESO-1 is highly expressed in myxoid and round cell subset of liposarcomas.

    Science.gov (United States)

    Hemminger, Jessica A; Ewart Toland, Amanda; Scharschmidt, Thomas J; Mayerson, Joel L; Kraybill, William G; Guttridge, Denis C; Iwenofu, O Hans

    2013-02-01

    Liposarcomas are a heterogenous group of fat-derived sarcomas, and surgery with or without chemoradiation therapy remains the main stay of treatment. NY-ESO-1 is a cancer-testis antigen expressed in various cancers where it can induce both cellular and humoral immunity. Immunotherapy has shown promise in clinical trials involving NY-ESO-1-expressing tumors. Gene expression studies have shown upregulation of the gene for NY-ESO-1, CTAG1B, in myxoid and round cell liposarcomas. Herein, we evaluated the expression of NY-ESO-1 among liposarcoma subtypes by quantitative real-time PCR, western blot analysis, and immunohistochemistry. Frozen tissue for quantitative real-time PCR and western blot analysis was obtained for the following liposarcoma subtypes (n=15): myxoid and round cell (n=8); well-differentiated (n=4), and dedifferentiated (n=3). Formalin-fixed paraffin-embedded blocks were obtained for the following liposarcoma subtypes (n=44): myxoid and round cell (n=18); well-differentiated (n=10); dedifferentiated (n=10); and pleomorphic (n=6). Full sections were stained with monoclonal antibody NY-ESO-1, and staining was assessed for intensity (1-3+), percentage of tumor positivity, and location. In all, 7/8 (88%) and 16/18 (89%) myxoid and round cell expressed CTAG1B and NY-ESO-1 by quantitative real-time PCR and immunohistochemistry, respectively. Western blot correlated with mRNA expression levels. By immunohistochemistry, 94% (15/16) of positive cases stained homogenously with 2-3+ intensity. Also, 3/6 (50%) pleomorphic liposarcomas demonstrated a range of staining: 1+ intensity in 50% of cells; 2+ intensity in 5% of cells; and 3+ intensity in 90% of cells. One case of dedifferentiated liposarcoma showed strong, diffuse staining (3+ intensity in 75% of cells). Our study shows that both CTAG1B mRNA and protein are overexpressed with high frequency in myxoid and round cell liposarcoma, enabling the potential use of targeted immunotherapy in the treatment of this

  16. Mo isotopes in OAE 2 black shales

    Science.gov (United States)

    Westermann, Stephane; Vance, Derek; Cameron, Vyllinniskii; Archer, Corey; Robinson, Stuart A.

    2014-05-01

    Sedimentary rocks, especially organic-rich deposits, have the potential to track change in the oxygenation state of the ocean over geological time. Oceanic anoxic events (OAEs) correspond to periods of profound and rapid environmental change, which have led to both the widespread deposition of black shales and the development of widespread anoxia in the ocean. Understanding the variations in redox conditions during these events is of primary importance, since recent observations and modelling have shown that processes invoked to explain the origin of OAEs are being observed today as a consequence of anthropogenic change. Here, we compare redox-sensitive trace metal (RSTM) distributions and molybdenum (Mo) isotope variations during a major Cretaceous OAE (OAE 2, Bonarelli event). Whereas RSTM have the potential to provide insights regarding local depositional conditions and processes in palaeoceanographic systems, Mo-isotope data can, under certain circumstances, provide quantitative estimates of how the global extent of seawater anoxia may have fluctuated in the past. We selected for study a series sections within the western Tethys (La Contessa and Furlo, Italy) and in the northern Atlantic (DSDP site 367, Cape Verde Basin and ODP site 1276, Newfoundland Basin. RSTM contents show similar trends through all the studied sections, characterized by low concentration below and above the OAE interval and higher concentrations within the Bonarelli interval. This suggests rapid variations in the redox conditions, from suboxic to euxinic conditions during OAE 2. The RSTM enrichment factors (EFs) indicate different depositional conditions and palaeoceanographic processes between the Tethys and the North Atlantic. Whereas the North Atlantic sites show evidence of weak watermass restriction associated with the action of a particulate shuttle within the water column, the EFs of the Tethyan sections are characteristic of unrestricted marine systems. Despite local differences in

  17. Biodistribution of 99Mo in rats

    Directory of Open Access Journals (Sweden)

    Raphael Sancho Sisley de Souza

    2008-12-01

    Full Text Available The modification of 99Mo standard metabolism in the presence of MDP would alter the dosimetry of this radionuclide in nuclear medicine patients. Therefore, the objective of this work is to evaluate the influence of MDP in the biodistribution of 99Mo. Wistar rats were divided in two groups of six animals, being inoculated respectively 99Molibdate and 99Mo+MDP via plex ocular. The biodistribution study was carried out after 10 and 120 minutes respectively. The organs were counted with a NaI(Tl detector. The uptake values did not present significant differences among the groups. An in vitro study through planar chromatography was carried out to determine the affinity between molybdenum and MDP. The results show that 99Mo has low affinity both to propanone and NaCl-0.9% solution. However, 99Mo in the presence of MDP presented affinity to NaCl-0.9% solution and low affinity to propanone suggesting that 99Mo was bound to MDP under the conditions of the experiment.A modificação do metabolismo padrão do 99Mo em presença de MDP levaria a alterações na dosimetria deste radionuclídeo em pacientes de medicina nuclear. Assim, o objetivo deste trabalho é avaliar a influência do MDP na biodistribuição de 99Mo. Ratos Wistar foram divididos em dois grupos de seis animais, sendo inoculados respectivamente com 99Molibdato e 99Molibdato+MDP via plexo ocular. O estudo de biodistribuição foi realizado após 10 e 120 minutos respectivamente. Os órgãos foram contados com detector NaI(Tl. Os valores de uptake não apresentaram diferenças significativas entre os grupos. Foi realizado um estudo in vitro através de cromatografia planar para determinar a afinidade entre o molibdênio e o MDP. Os resultados mostraram que o molibdênio tem baixa afinidade tanto pela propanona quanto pela solução 0.9% de NaCl. Entretanto, o molibdênio em presença de MDP apresentou afinidade pela solução 0.9% de NaCl e baixa afinidade pela propanona, sugering ter ocorrido

  18. Expression of NY-ESO-1 gene detected by RT-PCR in human gastric carcinoma and its gene cloning%RT-PCR检测胃癌NY-ESO-1抗原的表达及其基因克隆

    Institute of Scientific and Technical Information of China (English)

    黄梅花; 刘磊; 欧阳永忠

    2005-01-01

    目的研究NY-ESO-1基因在胃癌中的表达,以便了解胃癌患者是否可以使用NY-ESO-1抗原为基础的疫苗治疗.方法采用RT-PCR方法检测NY-ESO-1基因在60例胃癌组织和相应的癌旁正常组织中的表达,采用分子克隆的方法从胃癌组织中克隆了NY-ESO-1 cDNA.结果 NY-ESO-1基因在胃癌组织中的表达率为55%(33/60),在相应的癌旁正常组织中未见表达,克隆的NY-ESO-1cDNA序列与GenBank报道一致.结论 NY-ESO-1基因在胃癌中呈高表达率,NY-ESO-1抗原可以被具有HLA-1类分子限制性CTL识别.

  19. RT-PCR检测胃癌NY-ESO-1抗原的表达及其基因克隆%Expression of NY-ESO-1 gene was analyzed by RT-PCR in human gastric carcinoma and its cloning

    Institute of Scientific and Technical Information of China (English)

    黄梅花; 刘磊; 欧阳永忠

    2005-01-01

    目的:研究NY-ESO-1基因在胃癌中的表达,以便了解为胃癌患者是否可以使用NY-ESO-1抗原为基础的疫苗治疗.方法:采用RT-PCR方法检测了NY-ESO-1基因在60例胃癌组织和相应的癌旁正常组织中的表达,采用分子克隆的方法从胃癌组织中克隆了NY-ESO-1cDNA.结果:NY-ESO-1基因在胃癌组织中的表达率为55%(33/60),在相应的癌旁正常组织中的未见表达,克隆的NY-ESO-1cDNA序列与GenBank报道一致.结论:NY-ESO-1基因在胃癌中的高表达率,NY-ESO-1抗原可以被具有HLA-1类分子限制性CTL识别.

  20. Physico-chemical Characterization of Mo-Hβ Zeolite Catalysts

    Institute of Scientific and Technical Information of China (English)

    LIU Sheng-lin; HUANG Sheng-jun; XIN Wen-jie; QIN xin-hua; XIE Su-juan; XU Long-ya

    2004-01-01

    A series of Mo-impregnated Hβ samples, with MoO3 loading in Hβ zeolite in the mass fraction range of 0. 5%-6.0%, were studied by means of XRD and IR in order to characterize their structures. Mo/Hβ samples' crystallinity almost linearly decreases with increasing the amount of MoO3 loaded. The IR spectra and XRD patterns suggest that the progressive destabilization of the Hβ zeolite structure is caused by increasing Mo loading in (MoO3+Hβ zeolite). During the calcination, Al2(MoO4)3 formed from the dealumination of Hβ zeolite, causes the substantially partial breakdown of the zeolite framework when the Mo loading in MoO3 +Hβ is relatively high.

  1. Phase diagrams of Li2MoO4-Na2MoO4 and Na2MoO4-K2MoO4 systems

    Institute of Scientific and Technical Information of China (English)

    DING Yimin; HOU Na; CHEN Nianyi; XIA Yiben

    2006-01-01

    The phase diagrams of the Li2MoO4-Na2MoO4 and Na2MoO4-K2MoO4 systems have been reassessed using differential thermal analysis together with high-temperature and room-temperature X-ray diffraction analysis. The results showed that the compound Li2MoO4·6Na2MoO4 did not exist; however, it confirmed the existence of the compound Li2MoO4·3Na2MoO4 in the Li2MoO4-Na2MoO4 systems. With regard to the system of Na2MoO4-K2MoO4, we could not confirm the results reported by Bukhanova who claimed that the system was eutectic type with 1∶1 and 1∶2 intermediate compounds, refuting the statement of Amadori who thought there was an apparent phase boundary at high temperature in α-solid solution region of the Na2MoO4-K2MoO4 binary system. The revised phase diagrams of these systems are illustrated in this article. These experimental results are in agreement with the computerized prediction using the support vector machine-atomic parameter method for the assessment of phase diagrams.

  2. Recent results from MoNA-LISA

    Science.gov (United States)

    Spyrou, Artemisia

    2012-03-01

    Studies of the nuclear properties of nuclei close and even beyond the limits of stability have revealed exotic modes of decay and new structural characteristics. The MoNA-LISA array is used at the National Superconducting Cyclotron Laboratory at Michigan State University to study nuclei along the neutron dripline. In a typical experiment, a radioactive beam is employed to produce the neutron-unbound state of interest. This state/resonance immediately decay into a neutron, which is detected by MoNA-LISA and a remaining charged nucleus detected by the sweeper magnet detector suite. In this talk, new exciting findings from recent MoNA-LISA experiments will be presented. These include the first observation of a dineutron decay from ^16Be, the exploration of the ``south shore'' of the Island of Inversion and the first evidence of the decay of the troubling nucleus ^26O.

  3. On the spin modulated circular polarization from the intermediate polars NY Lup and IGRJ1509-6649

    CERN Document Server

    Potter, Stephen B; Kotze, Marissa; Zietsman, Ewald; Butters, O W; Pekeur, Nikki; Buckley, David A H

    2011-01-01

    We report on high time resolution, high signal/noise, photo-polarimetry of the intermediate polars NY Lup and IGRJ1509-6649. Our observations confirm the detection and colour dependence of circular polarization from NY Lup and additionally show a clear white dwarf, spin modulated signal. From our new high signal/noise photometry we have unambiguously detected wavelength dependent spin and beat periods and harmonics thereof. IGRJ1509-6649 is discovered to also have a particularly strong spin modulated circularly polarized signal. It appears double peaked through the I filter and single peaked through the B filter, consistent with cyclotron emission from a white dwarf with a relatively strong magnetic field. We discuss the implied accretion geometries in these two systems and any bearing this may have on the possible relationship with the connection between polars and soft X-ray-emitting IPs. The relatively strong magnetic fields is also suggestive of them being polar progenitors.

  4. Ansatte perspektiver på utvikling av tverrfaglighet i ny sykehusavdeling

    Directory of Open Access Journals (Sweden)

    Hildfrid V. Brataas

    2009-02-01

    Full Text Available Artikkelen omhandler en følgestudie om ansattes perspektiver på teamorganisert og brukerinvolvert tverrfaglig samarbeid i en ny rehabiliteringsavdeling i et norsk sykehus. Studiens formål var å bringe frem mer kunnskap om forhold av betydning ved utvikling av tverrfaglige arbeidsformer i sammensatte helsetjenester.Metode: Undersøkelsen var kvalitativt beskrivende og fortolkende med bruk av fokusgruppeintervjuer tre og fem år etter oppstart av rehabiliteringsavdelingen.Resultater: Over en femårsperiode var praksis erfart å endre karakter, fra flerfaglig samarbeid til funksjonelt tverrfaglig samarbeid. Profesjonsoverbyggende teori om rehabiliterings-virksomheten og ledelsens kvalitetsorientering var erfart som hjelp for teamenes utvikling av funksjonell tverrfaglighet.Konklusjon: Langsiktighet, kvalitetsorientert ledelse og teamorganisert rehabiliteringsarbeid basert på profesjonsoverbyggende teoriramme synes å underbygge utvikling av funksjonell tverrfaglighetsforståelse og praksis. Denne kunnskapen er relevant ved omlegging til teamorganisert rehabiliteringsvirksomhet i eksisterende og nye virksomheter. Det er behov for mer forskning om betydning av personfaktorer og motivasjon, ledelse og overbyggende teorirammeverk for samarbeidsutvikling og kvalitet i praksis.

  5. Integrated water vapor above Ny Ålesund, Spitsbergen: a multi-sensor intercomparison

    Directory of Open Access Journals (Sweden)

    M. Pałm

    2010-02-01

    Full Text Available Water vapor is an important constituent of the atmosphere. Because of its abundance and its radiative properties it plays an important role for the radiation budget of the atmosphere and has major influence on weather and climate.

    In this work integrated water vapor (IWV derived from the measurements of three satellite sensors, GOME, SCIAMACHY and AMSU-B, two ground based sensors, a Fourier-transform spectrometer (FTIR, a microwave radiometer for O3 (RAM and IWV inferred from GPS zenith path delay (ZPD measurements, are compared to radio-sonde measurements above Ny Ålesund, 79° N. All six remote sensors exploit different principles and work in different wavelength regions.

    All remote sensing instruments reproduce the sonde measurements very well and are highly correlated when compared with the radio-sonde measurements.

    The ground-based FTIR shows very little scatter of about 10%. The GPS performs similar to the FTIR at all times except for very low IWV, where the scatter exceeds 50% of the measured IWV. The other remote sensing instruments show scatter of about 20% (standard deviation. The ground-based RAM performs similar to the satellite instruments, despite the fact that the retrieval of IWV is just a by-product of this ozone sensor.

  6. Characterization of a Single Magnetotactic Bacterial Species from Devil's Bathtub, Mendon Ponds Park, Honeoye Falls, NY

    Science.gov (United States)

    Wagner, C.; Tarduno, J. A.; Stein, A.; Sia, E.

    2015-12-01

    Magnetotactic bacteria (MTB) belong to a lineage of prokaryotic bacteria that synthesize magnetosomes, single domain magnetic particles (typically magnetite or greigite) with an average size of 50 nanometers. MTB utilize magnetosomes through magnetotaxis, the alignment and movement along magnetic field lines to navigate towards preferred environmental conditions. MTB are sensitive to different environments and are thought to exhibit varying magnetosome morphologies, compositions, sizes, and quantities in regards to the environments which they inhabit. These characteristics allow MTB and magnetofossils (preserved magnetosomes) to be used as modern/paleoenvironmental recorders and biomarkers for environmental change(s). Devil's Bathtub (Mendon Ponds Park, Honeoye Falls, NY) is a meromictic glacial kettle pond surrounded by deciduous tree cover. Here we examine one species of MTB based on prominence of this particular morphology at this locale. Magnetotaxis and morphology of this species have been observed using light microscopy. Micrographs have also been taken using Transmission Electron Microscopy (TEM) to verify cell morphology and to determine magnetosome morphology. TEM and magnetic hysteresis measurements were done to find and test the composition of magnetosomes. In this study we also focus on DNA sequencing and characterization of this MTB, as there are few MTB species which have been DNA sequenced successfully. Data from these experiments are directly applicable to this up-and-coming area of research as it will aid in the understanding and correlation of magnetosome and magnetofossils with environmental characteristics.

  7. Single-particle characterization of summertime arctic aerosols collected at Ny-Alesund, Svalbard.

    Science.gov (United States)

    Geng, Hong; Ryu, Jiyeon; Jung, Hae-Jin; Chung, Hyeok; Ahn, Kang-Ho; Ro, Chul-Un

    2010-04-01

    Single-particle characterization of summertime Arctic aerosols is useful to understand the impact of air pollutants on the polar atmosphere. In the present study, a quantitative single particle analytical technique, low-Z particle electron probe X-ray microanalysis, was used to characterize 8100 individual particles overall in 16 sets of aerosol samples collected at Ny-Alesund, Svalbard, Norway on 25-31 July, 2007. Based on their X-ray spectral and secondary electron image data of individual particles, 13 particle types were identified, in which particles of marine origin were the most abundant, followed by carbonaceous and mineral dust particles. A number of aged (reacted) sea salt (and mixture) particles produced by the atmospheric reaction of genuine sea-salts, especially with NO(x) or HNO(3), were significantly encountered in almost all the aerosol samples. They greatly outnumbered genuine sea salt particles, implying that the summertime Arctic atmosphere, generally regarded as a clean background environment, is disturbed by anthropogenic air pollutants. The main sources of airborne NO(x) (or HNO(3)) are probably ship emissions around the Arctic Ocean, industry emission from northern Europe and northwestern Siberia, and renoxification of NO(3)(-) within or on the melting snow/ice surface.

  8. Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA.

    Science.gov (United States)

    Tei, Freda F; Kowalyk, Steven; Reid, Jhenelle A; Presta, Matthew A; Yesudas, Rekha; Mayer, D C Ghislaine

    2016-03-29

    Bivalves have been shown to be carriers of the human intestinal parasites Cryptosporidium parvum and Toxoplasma gondii. The goal of this study is to determine the prevalence of protozoan parasites in mollusks of New York City using a polymerase chain reaction (PCR)-based assay. Four species of mollusks, Mya arenaria, Geukensia demissa, Crassostrea virginica, and Mytilis edulis, were collected from Orchard Beach, NY in the fall of 2014, totaling 159 specimens. Each individual mollusk was dissected to harvest the digestive gland, the mantle, the gills, the foot and the siphon. The tissues were assayed for the presence of Cryptosporidium parvum, Giardia lamblia, and Toxoplasma gondii DNA by using primers that target parasite-specific genes. C. parvum was found at a prevalence of 50%, 11.3%, and 1%, respectively, in Mya arenaria, G. demissa, and Mytilis edulis. C. parvum DNA was detected in all the tissues of these bivalve species, except the gills. Furthermore, G. lamblia was detected in Mya arenaria, G. demissa, Crassostrea virginica and Mytilis edulis at a prevalence of 37.5%, 4.5%, 60%, and 20.6%, respectively, while T. gondii DNA was not detected.

  9. Local Volume TiNy Titans: Gaseous Dwarf-Dwarf Interactions in the Local Universe

    CERN Document Server

    Pearson, Sarah; Putman, Mary E; Lutz, Katharina A; Fernandez, Ximena; Stierwalt, Sabrina; Patton, David R; Kim, Jinhyub; Kallivayalil, Nitya; Johnson, Kelsey; Sung, Eon-Chang

    2016-01-01

    In this paper, we introduce the Local Volume TiNy Titans sample (LV-TNT), which is a part of a larger body of work on interacting dwarf galaxies: TNT (Stierwalt et al. 2015). This LV-TNT sample consists of 10 dwarf galaxy pairs in the Local Universe (< 30 Mpc from Milky Way), which span mass ratios of M_(*,1)/M_(*,2) < 20, projected separations < 100 kpc, and pair member masses of log(M_*/M_Sun) < 9.9. All 10 LV-TNT pairs have resolved synthesis maps of their neutral hydrogen, are located in a range of environments and captured at various interaction stages. This enables us to do a comparative study of the diffuse gas in dwarf-dwarf interactions and disentangle the gas lost due to interactions with halos of massive galaxies, from the gas lost due to mutual interaction between the dwarfs. We find that the neutral gas is extended in the interacting pairs when compared to non-paired analogs, indicating that gas is tidally pre-processed. Additionally, we find that the environment can shape the HI dist...

  10. Determination of Conduction and Valence Band Electronic Structure of LaTiOx Ny Thin Film.

    Science.gov (United States)

    Pichler, Markus; Szlachetko, Jakub; Castelli, Ivano E; Marzari, Nicola; Döbeli, Max; Wokaun, Alexander; Pergolesi, Daniele; Lippert, Thomas

    2017-05-09

    The nitrogen substitution into the oxygen sites of several oxide materials leads to a reduction of the band gap to the visible-light energy range, which makes these oxynitride semiconductors potential photocatalysts for efficient solar water splitting. Oxynitrides typically show a different crystal structure compared to the pristine oxide material. As the band gap is correlated to both the chemical composition and the crystal structure, it is not trivial to distinguish which modifications of the electronic structure induced by the nitrogen substitution are related to compositional and/or structural effects. Here, X-ray emission and absorption spectroscopy are used to investigate the electronic structures of orthorhombic perovskite LaTiOx Ny thin films in comparison with films of the pristine oxide LaTiOx with similar orthorhombic structure and cationic oxidation state. Experiment and theory show the expected upward shift in energy of the valence band maximum that reduces the band gap as a consequence of the nitrogen incorporation. This study also shows that the conduction band minimum, typically considered almost unaffected by nitrogen substitution, undergoes a significant downward shift in energy. For a rational design of oxynitride photocatalysts, the observed changes of both the unoccupied and occupied electronic states have to be taken into account to justify the total band-gap narrowing induced by the nitrogen incorporation. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. Aromaticity of Heterofullerenes C18BxNy (x+y=2) and Their Molecular Ions

    Institute of Scientific and Technical Information of China (English)

    Jia-li Chen; Ablikim Kerim

    2008-01-01

    The axomaticity of all possible substituted fullerene isomers of C18N2,C18N2,C18BN,and their molecular ions which originate from the C20 (Ih) cage were studied by the topological resonance energy (TRE) and the percentage topological resonance energy methods.The relationship between the aromaticity of C18BxNy isomers and the sites where the heteroatoms dope at the C20 (Ih) cage is discussed.Calculation results show that at the neutral and cationic states all the isomers axe predicted to be antiaromatic with negative TREs,but their polyvalent anions are predicted to be aromatic with positive TREs.The most stable isomer is formed by heteroatom doping at the 1,11-sites in C18N2,C18B2,and C18BN.Heterofullerenes are more aromatic than C20.The stability order in the neutral states is C18N2>C18BN>C18B2>C20.The stability order in closed-shell is C18B28->C206->C18BN6->C18N24-.This predicts theoretically that their polyvalent anions have high aromaticity.

  12. Everyday Physical Activity of Students in Nyíregyháza

    Directory of Open Access Journals (Sweden)

    Fintor János Gábor

    2015-05-01

    Full Text Available The popularity of physical education lessons has already been demonstrated by a lot of essays, however, it has also been revealed that this popularity, as well as the frequency of doing sports, tends to decrease at later ages of life. Pursuing sports has a positive effect on academic performance. Introducing PE as an everyday lesson at schools was a milestone in physical education. Provided the aims are realised successfully, the general state of health of students can show a favourable change. The aim of our research, on the one hand, is to reveal how important physical education is as contrasted with other school subjects, according to those students asked. On the other hand, we aim to examine how the certain positive and negative attitudes of the subjects of our study appear in the resulting figures. The research was conducted in the form of self-completion questionnaires in four different funded institutions in Nyíregyháza (n=285, in May, 2014. In the course of evaluation, we have used multivariate function analysis as well as frequency research and cross table reference. Participants mainly regard PE as important as other school subjects, however, boys significantly regard it more important than girls do. Those who do sports on a regular basis were more likely to agree that PE lessons are conducted in a friendly atmosphere than those who do sports less. Significantly, boys tended to agree with positive statements about sport.

  13. Rugby-metoden SCRUM: En ny måde at bedrive projekter på

    DEFF Research Database (Denmark)

    Pries-Heje, Lene; Pries-Heje, Jan

    2012-01-01

    En ny måde at bedrive projekter på breder sig i disse år med overraskende fart. Selv om de første tanker om ”Scrum” – hvor navnet og fremgangsmåden er inspireret af Rugby-spillet – blev tænkt for mere end 10 år siden, så er det først nu at fremgangsmåden for alvor har vundet udbredelse i Danmark....... Denne artikel gennemgår metoden og fortæller om både positive resultater og udfordringer i tre danske organisationer: Et softwarehus, en bank, og et universitet. De tre cases illustrer meget forskellige anvendelsessituationer af Scrum set i forhold til projekternes størrelse, karakter og den...... organisatoriske kontekst. Artiklen slutter med at diskutere, hvad det er der gør, at Scrum er blevet så populær, og i dag fremstår som et reelt alternativ til klassisk plandreven projektledelse...

  14. Hudson River Paleoclimate, Sea Level, and Human Impact: A Record From Piermont Marsh, NY

    Science.gov (United States)

    Kurdyla; Peteet, Dorothy; Liberman, Louisa; Sugar; Wong; Hansen, James E. (Technical Monitor)

    2001-01-01

    A 13.77 meter sediment core from Piermont Marsh, NY (40 00 N, 73 55W) records the local and regional vegetational and foraminiferal history of the Hudson Estuary. The sediments were sampled every 4 cm, which represents a decadal to centuryscale resolution. Basal sediment dating is in progress, and the 11-m depth represents about 4000 years. Changes in plant macrofossils and charcoal appear to indicate differences in salinIty and drought, suggesting changes in climate. Scirpus, Salicornia, and high levels of charcoal seem to indicate drier/more saline conditions, while lack of these macrofossils and increases in Chara/Nitella, aquatic leaves, and very little charcoal suggests wetter conditions. Other macrofossils include Carex, Juncus, Polygonum, Zanichellia, Ruppia. High resolution AMS dating of plant macrofossils is in progress, and will be compared with changes in Hudson River sediment cores offshore. Foraminiferal assemblages from key intervals of the core will be presented. Human impact in the upper sediments is visible from the influx of grass seeds, primarily Phragmites, and the ragweed pollen rise.

  15. Water residence times and nutrient budgets across an urbanizing gradient (Croton water supply area, NY)

    Science.gov (United States)

    Vitvar, T.; Burns, D. A.; Duncan, J. M.; Hassett, J. M.; Mitchell, M. J.

    2002-05-01

    Water residence times and nutrient budgets in 3 small watersheds in the Croton water supply area, NY, were examined. The watersheds (less than 1km 2) have different level of urbanization (natural, semi-developed and fully developed), different mechanisms of runoff generation (quick flow on roads and slow flow through subsurface) and different watershed landscape characteristics (wet zones, hillslopes) . Measurements of the comprehensive chemical suite incl. components of nitrogen budget in the throughfall, stream water, soil water and groundwater in the saturated zone were performed bi-weekly over a period up to 2 years. Mean water residence times of the stream water were estimated using Oxygen-18 and Helium-3/Tritium isotopes. There are significant differences in the chemical composition and decrease of nitrification intensity and of mean streamwater residence time along the increasing watershed development. Within each watershed, longer water residence times (up to over 2 years) were estimated in the wetland zones without direct communication with streams in comparison to hillslope areas (up to over 1 year). The results can be used in watershed management and planning of the further urbanization of this water supply area.

  16. Aromaticity of Heterofullerenes C18BxNy (x + y = 2) and Their Molecular Ions

    Science.gov (United States)

    Chen, Jia-li; Kerim, Ablikim

    2008-04-01

    The aromaticity of all possible substituted fullerene isomers of C18N2, C18B2, C18BN, and their molecular ions which originate from the C20 (Ih) cage were studied by the topological resonance energy (TRE) and the percentage topological resonance energy methods. The relationship between the aromaticity of C18BxNy isomers and the sites where the heteroatoms dope at the C20 (Ih) cage is discussed. Calculation results show that at the neutral and cationic states all the isomers are predicted to be antiaromatic with negative TREs, but their polyvalent anions are predicted to be aromatic with positive TREs. The most stable isomer is formed by heteroatom doping at the 1, 11-sites in C18N2, C18B2, and C18BN. Heterofullerenes are more aromatic than C20. The stability order in the neutral states is C18N2 > C18 BN > C18 B2 > C20. The stability order in closed-shell is C18B28- > C206- > C18BN6- > C18N24-. This predicts theoretically that their polyvalent anions have high aromaticity.

  17. Elevated Temperature Tensile Tests on DU–10Mo Rolled Foils

    Energy Technology Data Exchange (ETDEWEB)

    Schulthess, Jason [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2014-09-01

    Tensile mechanical properties for uranium-10 wt.% molybdenum (U–10Mo) foils are required to support modeling and qualification of new monolithic fuel plate designs. It is expected that depleted uranium-10 wt% Mo (DU–10Mo) mechanical behavior is representative of the low enriched U–10Mo to be used in the actual fuel plates, therefore DU-10Mo was studied to simplify material processing, handling, and testing requirements. In this report, tensile testing of DU-10Mo fuel foils prepared using four different thermomechanical processing treatments were conducted to assess the impact of foil fabrication history on resultant tensile properties.

  18. Formulation of Organic Fertilizer Standard in Jiaxing City Based on NY525—2012

    OpenAIRE

    HUANG, Jinfa; NI, Xiongwei; Xie, Jianping; Shi, Yanping

    2013-01-01

    On the basis of research, analysis and argumentation, we conduct analysis and research on the current situation of quality of commercial organic fertilizer in Jiaxing City, and carry out the inspection and testing of organic matter indicators using the laboratory. Based on the standard of NY525-2012 Organic Fertilizer by the Ministry of Agriculture, this paper formulates the quality level indicators of local commercial organic fertilizer and adding specifications of raw materials.

  19. Evaluation of Photocatalytic Properties of Portland Cement Blended with Titanium Oxynitride (TiO2−xNy Nanoparticles

    Directory of Open Access Journals (Sweden)

    Juan D. Cohen

    2015-07-01

    Full Text Available Photocatalytic activity of Portland cement pastes blended with nanoparticles of titanium oxynitride (TiO2−xNy was studied. Samples with different percentages of TiO2−xNy (0.0%, 0.5%, 1%, 3% and TiO2 (1%, 3% were evaluated in order to study their self-cleaning properties. The presence of nitrogen in the tetragonal structure of TiO2 was evidenced by X-ray diffraction (XRD as a shift of the peaks in the 2θ axis. The samples were prepared with a water/cement ratio of 0.5 and a concentration of Rhodamine B of 0.5 g/L. After 65 h of curing time, the samples were irradiated with UV lamps to evaluate the reduction of the pigment. The color analysis was carried out using a Spectrometer UV/Vis measuring the coordinates CIE (Commission Internationale de l’Eclairage L*, a*, b*, and with special attention to the reddish tones (Rhodamine B color which correspond to a* values greater than zero. Additionally, samples with 0.5%, 1%, 3% of TiO2−xNy and 1%, 3% of TiO2 were evaluated under visible light with the purpose of determining the Rhodamine B abatement to wavelengths greater than 400 nm. The results have shown a similar behavior for both additions under UV light irradiation, with 3% being the addition with the highest photocatalytic efficiency obtained. However, TiO2−xNy showed activity under irradiation with visible light, unlike TiO2, which can only be activated under UV light.

  20. Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

    Science.gov (United States)

    Dyrskjøt, L; Zieger, K; Kissow Lildal, T; Reinert, T; Gruselle, O; Coche, T; Borre, M; Ørntoft, T F

    2012-01-01

    Background: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. Methods: We used specific q-RT-PCR assays to analyse the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. Results: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P=0.026), LAGE-1 (P=0.001) and NY-ESO-1 (P=0.040) was significantly associated with a shorter progression-free survival. In addition, we found that patients with tumours expressing PRAME responded poorly to chemotherapy (P=0.02, χ2-test). Conclusion: Cancer-testis genes are frequently expressed in bladder cancer and especially in tumours of high stage and grade. In addition, the CT gene expression may have both prognostic and predictive value. Development of specific immunotherapy against the CT antigens in bladder cancer may ultimately increase patient survival. PMID:22596240

  1. Ny løn: Den centrale vision der fik det svært ved det lokale forhandlingsbord

    DEFF Research Database (Denmark)

    Ravn, Ib; Møller, Anna Marie

    2001-01-01

    afdelingsleder og tillidsrepræsentant. Lokale ledere og tillidsrepræsentanter har derfor måttet optræde i en ny rolle som lønforhandlere. Det har medført et stort behov for viden om, hvordan man gennemfører en forhandling om løn på den enkelte arbejdsplads. Hvordan starter man og hvordan slutter man? Hvordan...

  2. Rapid generation of NY-ESO-1-specific CD4+ THELPER1 cells for adoptive T-cell therapy

    Science.gov (United States)

    Kayser, Simone; Boβ, Cristina; Feucht, Judith; Witte, Kai-Erik; Scheu, Alexander; Bülow, Hans-Jörg; Joachim, Stefanie; Stevanović, Stefan; Schumm, Michael; Rittig, Susanne M; Lang, Peter; Röcken, Martin; Handgretinger, Rupert; Feuchtinger, Tobias

    2015-01-01

    Tumor-associated antigens such as NY-ESO-1 are expressed in a variety of solid tumors but absent in mature healthy tissues with the exception of germline cells. The immune system anti-cancer attack is mediated by cell lysis or induction of growth arrest through paralysis of tumor cells, the latter of which can be achieved by tumor-specific CD4+, IFNγ-producing THelper type 1 (TH1) cells. Translation of these immune-mediated mechanisms into clinical application has been limited by availability of immune effectors, as well as the need for complex in vitro protocols and regulatory hurdles. Here, we report a procedure to generate cancer-testis antigen NY-ESO-1-targeting CD4+ TH1 cells in vitro for cancer immunotherapy in the clinic. After in vitro sensitization by stimulating T cells with protein-spanning, overlapping peptide pools of NY-ESO-1 in combination with IL-7 and low dose IL-2, antigen-specific T cells were isolated using IFNγ capture technique and subsequently expanded with IL-2, IL-7 and IL-15. Large numbers of NY-ESO-1-specific CD4+ T cells with a TH1 cytokine profile and lower numbers of cytokine-secreting CD8+ T cells could be generated from healthy donors with a high specificity and expansion potential. Manufactured CD4+ T cells showed strong specific TH1-responses with IFNγ+, TNFα+, IL-2+ and induced cell cycle arrest and apoptosis in tumor cells. The protocol is GMP-grade and approved by the regulatory authorities. The tumor-antigen specific CD4+ TH1 lymphocytes can be adoptively transferred as a T-cell therapy to boost anticancer immunity and this novel cancer treatment approach is applicable to both T cells from healthy allogeneic donors as well as to autologous T cells derived from cancer patients. PMID:26155389

  3. Rapid generation of NY-ESO-1-specific CD4(+) THELPER1 cells for adoptive T-cell therapy.

    Science.gov (United States)

    Kayser, Simone; Boβ, Cristina; Feucht, Judith; Witte, Kai-Erik; Scheu, Alexander; Bülow, Hans-Jörg; Joachim, Stefanie; Stevanović, Stefan; Schumm, Michael; Rittig, Susanne M; Lang, Peter; Röcken, Martin; Handgretinger, Rupert; Feuchtinger, Tobias

    2015-05-01

    Tumor-associated antigens such as NY-ESO-1 are expressed in a variety of solid tumors but absent in mature healthy tissues with the exception of germline cells. The immune system anti-cancer attack is mediated by cell lysis or induction of growth arrest through paralysis of tumor cells, the latter of which can be achieved by tumor-specific CD4(+), IFNγ-producing THelper type 1 (TH1) cells. Translation of these immune-mediated mechanisms into clinical application has been limited by availability of immune effectors, as well as the need for complex in vitro protocols and regulatory hurdles. Here, we report a procedure to generate cancer-testis antigen NY-ESO-1-targeting CD4(+) TH1 cells in vitro for cancer immunotherapy in the clinic. After in vitro sensitization by stimulating T cells with protein-spanning, overlapping peptide pools of NY-ESO-1 in combination with IL-7 and low dose IL-2, antigen-specific T cells were isolated using IFNγ capture technique and subsequently expanded with IL-2, IL-7 and IL-15. Large numbers of NY-ESO-1-specific CD4(+) T cells with a TH1 cytokine profile and lower numbers of cytokine-secreting CD8(+) T cells could be generated from healthy donors with a high specificity and expansion potential. Manufactured CD4(+) T cells showed strong specific TH1-responses with IFNγ(+), TNFα(+), IL-2(+) and induced cell cycle arrest and apoptosis in tumor cells. The protocol is GMP-grade and approved by the regulatory authorities. The tumor-antigen specific CD4(+) TH1 lymphocytes can be adoptively transferred as a T-cell therapy to boost anticancer immunity and this novel cancer treatment approach is applicable to both T cells from healthy allogeneic donors as well as to autologous T cells derived from cancer patients.

  4. The New York City Urban Search and Rescue Team (NY-TF1): A Case Study of Interagency Effectiveness

    Science.gov (United States)

    2011-03-01

    can be incorporated into both the FDNY and NYPD responses to everyday multi-agency operations. Applying the appreciative inquiry (AI) method into...this thesis utilized appreciative inquiry in a single case study of the NY-TF1 model supported by a set of seven interviews with key task force...collaborative elements developed through an appreciative inquiry of the task force will provide a vision for future interagency operations between NYC’s

  5. Effect of Sintering Temperature of Mo Skeleton on the Contiguity and Compressive Properties of Mo-Cu IPCs

    Science.gov (United States)

    Liu, Longfei; Cao, Fuhua; Lu, Liwei; Yan, Jianhui

    2017-02-01

    Interpenetrating phase composites (IPCs) are interesting materials, in which each phase can contribute its most desirable attributes to the composite as a whole by its contiguous morphology. In the present study, molybdenum-copper (Mo-Cu) composites with interpenetrating microstructure were fabricated by open-celled porous Mo skeleton with infiltration of Cu. Effects of sintering temperature on the Mo-Mo contiguity and compressive properties of the Mo-Cu IPCs were examined. The contiguity of Mo-Mo and compressive strength increase with the sintering temperature increasing from 1473 to 1873 K, and decrease at 2073 K. Volume fraction of Cu infiltrated in Mo skeleton and failure strain of composites decrease with the sintering temperature increasing from 1473 to 1873 K and increase at 2073 K. Mutual dependency of compressive properties and contiguity of Mo-Cu IPCs are illustrated.

  6. Oxidation, Reduction, and Condensation of Alcohols over (MO3)3 (M=Mo, W) Nanoclusters

    Energy Technology Data Exchange (ETDEWEB)

    Fang, Zongtang; Li, Zhenjun; Kelley, Matthew S.; Kay, Bruce D.; Li, Shenggang; Hennigan, Jamie M.; Rousseau, Roger J.; Dohnalek, Zdenek; Dixon, David A.

    2014-10-02

    The reactions of deuterated methanol, ethanol, 1-propanol, 1-butanol, 2-propanol, 2-butanol and t-butanol over cyclic (MO3)3 (M = Mo, W) clusters were studied experimentally with temperature programmed desorption (TPD) and theoretically with coupled cluster CCSD(T) theory and density functional theory. The reactions of two alcohols per M3O9 cluster are required to provide agreement with experiment for D2O release, dehydrogenation and dehydration. The reaction begins with the elimination of water by proton transfers and forms an intermediate dialkoxy species which can undergo further reaction. Dehydration proceeds by a β hydrogen transfer to a terminal M=O. Dehydrogenation takes place via an α hydrogen transfer to an adjacent MoVI = O atom or a WVI metal center with redox involved for M = Mo and no redox for M = W. The two channels have comparable activation energies. H/D exchange to produce alcohols can take place after olefin is released or via the dialkoxy species depending on the alcohol and the cluster. The Lewis acidity of the metal center with WVI being larger than MoVI results in the increased reactivity of W3O9 over Mo3O9 for dehydrogenation and dehydration.

  7. P10_Nov2012_Oct2014: Fire Island, NY pre- and post- storm cross-shore profiles from November 2012 to October 2014.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This spreadsheet consists of Fire Island, NY pre- and post- storm cross-shore profiles collected from November 2012 to October 2014. This dataset contains a set of...

  8. P10_Nov2012_Oct2014: Fire Island, NY pre- and post- storm cross-shore profiles from November 2012 to October 2014.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This spreadsheet consists of Fire Island, NY pre- and post- storm cross-shore profiles collected from November 2012 to October 2014. This dataset contains a set of...

  9. Archive of Boomer and Sparker Data Collected During USGS Cruise DIAN 97032 Long Island, NY Inner Shelf -- Fire Island, 24 September - 19 October, 1997

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS Diane G 97032 cruise. The coverage is the nearshore of Long Island, NY...

  10. Archive of Boomer and Sparker Data Collected During USGS Cruise DIAN 97032 Long Island, NY Inner Shelf -- Fire Island, 24 September - 19 October, 1997

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS Diane G 97032 cruise. The coverage is the nearshore of Long Island, NY...

  11. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer.

    Science.gov (United States)

    Fonteneau, Jean Francois; Brilot, Fabienne; Münz, Christian; Gannagé, Monique

    2016-01-01

    NY-ESO-1-specific CD4(+) T cells are of interest for immune therapy against tumors, because it has been shown that their transfer into a patient with melanoma resulted in tumor regression. Therefore, we investigated how NY-ESO-1 is processed onto MHC class II molecules for direct CD4(+) T cell recognition of melanoma cells. We could rule out proteasome and autophagy-dependent endogenous Ag processing for MHC class II presentation. In contrast, intercellular Ag transfer, followed by classical MHC class II Ag processing via endocytosis, sensitized neighboring melanoma cells for CD4(+) T cell recognition. However, macroautophagy targeting of NY-ESO-1 enhanced MHC class II presentation. Therefore, both elevated NY-ESO-1 release and macroautophagy targeting could improve melanoma cell recognition by CD4(+) T cells and should be explored during immunotherapy of melanoma. Copyright © 2015 by The American Association of Immunologists, Inc.

  12. Blockade of CTLA-4 promotes the development of effector CD8+ T lymphocytes and the therapeutic effect of vaccination with an attenuated protozoan expressing NY-ESO-1.

    Science.gov (United States)

    Dos Santos, Luara Isabela; Galvão-Filho, Bruno; de Faria, Paula Cristina; Junqueira, Caroline; Dutra, Miriam Santos; Teixeira, Santuza Maria Ribeiro; Rodrigues, Maurício Martins; Ritter, Gerd; Bannard, Oliver; Fearon, Douglas Thomas; Antonelli, Lis Ribeiro; Gazzinelli, Ricardo Tostes

    2015-03-01

    The development of cancer immunotherapy has long been a challenge. Here, we report that prophylactic vaccination with a highly attenuated Trypanosoma cruzi strain expressing NY-ESO-1 (CL-14-NY-ESO-1) induces both effector memory and effector CD8(+) T lymphocytes that efficiently prevent tumor development. However, the therapeutic effect of such a vaccine is limited. We also demonstrate that blockade of Cytotoxic T Lymphocyte Antigen 4 (CTLA-4) during vaccination enhances the frequency of NY-ESO-1-specific effector CD8(+) T cells producing IFN-γ and promotes lymphocyte migration to the tumor infiltrate. As a result, therapy with CL-14-NY-ESO-1 together with anti-CTLA-4 is highly effective in controlling the development of an established melanoma.

  13. Narrativ kostvejledning - en ny tilgang til diætisten, der styrker relationsdannelse og samarbejde med borgeren

    DEFF Research Database (Denmark)

    Kristensen, Søren Tange

    2017-01-01

    I Oplægget præsenteres hovedresultater fra en artikel indsendt til tidsskriftet Topics in Clinical Nutrition1, der undersøger hvordan Narrativ kostvejledning (NK) kan styrke diætisters relationsdannelse og samarbejde med borgere med kroniske lidelser. Artiklen bygger på data indsamlet i forbindelse...... har kroniske lidelser og hvilken betydning det har for borgernes fastholdelse i vejledningen samt deres sundhed og evne til at leve med deres kroniske lidelse(r). Dataindsamling fra denne del af projektet forventes afsluttet i efteråret 2017. 1: Søndergaard NJ, Lisbeth Kaufmann L, Hennesser Y...

  14. Source of and potential bio-indicator for the heavy metal pollution in Ny-(A)lesund, Arctic

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Three kinds of tundra plant samples including Dicranum angnstum(a type of boreal bryophyte) , PuccineUia phryganodes (a type of fringy p/ant),Salix polaris (a type of vascular plant) and surface soil were samples in 200 at Ny-Alesund of the Arctic.The levels of eight heavy metal elements (Hg, Pb, Cd, Cu, Zn, Ni, Fe and Mn) and three metal-like dements (As, Se, Sr) in the plant and soil samples of the areas within previous coal mining activities are significantly higher than those of other areas.The relative accumulation of these elements in these tundra plant samples is consistent with the one in the soft samples, especially in the areas affected by previous coal-mining activities.Thus, the pollution is apparently from local coal mining activity.Dicranum angustum has the highest concentrations among those elements, and it can be a good bio-indicator for heavy metal pollution in Ny(A)lesund.Though Ny(A)lesund is less polluted by heavy metal than nearby Northern European human living areas, but much more than the tundras of the Alaska, Greenland and the Antarctic.

  15. a-SiCxNy:H thin films for applications in solar cells as passivation and antireflective coatings

    Science.gov (United States)

    Swatowska, Barbara; Kluska, Stanisława; Lewińska, Gabriela; Golańska, Julia; Stapiński, Tomasz

    2016-12-01

    Amorphous a-SiCxNy:H thin films may be an alternative to a-Si:N:H coatings which are commonly used in silicon solar cells. This material was obtained by PECVD (13.56 MHz) method. The reaction gases used: silane, methane, nitrogen and ammonia. The structure of the layers were investigated by scanning electron microscopy (SEM) and infrared spectroscopy (FTIR). IR absorption spectra of a-SiCxNy:H layers confirmed the presence of various hydrogen bonds - it is important for passivation of Si structural defects. The ellipsometric measurements were implemented to determine the thickness of layers d, refractive index n, extinction coefficient k and energy gap Eg. The values of the energy gap of a-SiCxNy:H layers are in the range from 1.89 to 4.34 eV. The correlation between energy gap of materials and refractive index was found. Generally the introduction of N and/or C into the amorphous silicon network rapidly increases the Eg values.

  16. All-fiber femtosecond laser providing 9 nJ, 50 MHz pulses at 1650 nm for three-photon microscopy

    Science.gov (United States)

    Cadroas, P.; Abdeladim, L.; Kotov, L.; Likhachev, M.; Lipatov, D.; Gaponov, D.; Hideur, A.; Tang, M.; Livet, J.; Supatto, W.; Beaurepaire, E.; Février, S.

    2017-06-01

    The spectral window lying between 1.6 and 1.7 μm is interesting for in-depth multiphoton microscopy of intact tissues due to reduced scattering and absorption in this wavelength range. However, wide adoption of this excitation range will rely on the availability of robust and cost-effective high peak power pulsed lasers operating at these wavelengths. In this communication, we report on a monolithically integrated high repetition rate (50 MHz) all-fiber femtosecond laser based on a soliton self-frequency shift providing 9 nJ, 75 fs pulses at 1650 nm. We illustrate its potential for biological microscopy by recording three-photon-excited fluorescence and third-harmonic generation images of mouse nervous tissue and developing Drosophila embryos labeled with a red fluorescent protein.

  17. The modulation of Schottky barriers of metal-MoS2 contacts via BN-MoS2 heterostructures.

    Science.gov (United States)

    Su, Jie; Feng, Liping; Zhang, Yan; Liu, Zhengtang

    2016-06-22

    Using first-principles calculations within density functional theory, we systematically studied the effect of BN-MoS2 heterostructure on the Schottky barriers of metal-MoS2 contacts. Two types of FETs are designed according to the area of the BN-MoS2 heterostructure. Results show that the vertical and lateral Schottky barriers in all the studied contacts, irrespective of the work function of the metal, are significantly reduced or even vanish when the BN-MoS2 heterostructure substitutes the monolayer MoS2. Only the n-type lateral Schottky barrier of Au/BN-MoS2 contact relates to the area of the BN-MoS2 heterostructure. Notably, the Pt-MoS2 contact with n-type character is transformed into a p-type contact upon substituting the monolayer MoS2 by a BN-MoS2 heterostructure. These changes of the contact natures are ascribed to the variation of Fermi level pinning, work function and charge distribution. Analysis demonstrates that the Fermi level pinning effects are significantly weakened for metal/BN-MoS2 contacts because no gap states dominated by MoS2 are formed, in contrast to those of metal-MoS2 contacts. Although additional BN layers reduce the interlayer interaction and the work function of the metal, the Schottky barriers of metal/BN-MoS2 contacts still do not obey the Schottky-Mott rule. Moreover, different from metal-MoS2 contacts, the charges transfer from electrodes to the monolayer MoS2, resulting in an increment of the work function of these metals in metal/BN-MoS2 contacts. These findings may prove to be instrumental in the future design of new MoS2-based FETs with ohmic contact or p-type character.

  18. Functional T cells targeting NY-ESO-1 or Melan-A are predictive for survival of patients with distant melanoma metastasis.

    Science.gov (United States)

    Weide, Benjamin; Zelba, Henning; Derhovanessian, Evelyna; Pflugfelder, Annette; Eigentler, Thomas K; Di Giacomo, Anna Maria; Maio, Michele; Aarntzen, Erik H J G; de Vries, I Jolanda M; Sucker, Antje; Schadendorf, Dirk; Büttner, Petra; Garbe, Claus; Pawelec, Graham

    2012-05-20

    To analyze the prognostic relevance of circulating T cells responding to NY-ESO-1, Melan-A, MAGE-3, and survivin in patients with melanoma with distant metastasis. We examined 84 patients with follow-up after analysis (cohort A), 18 long-term survivors with an extraordinarily favorable course of disease before analysis (> 24 months survival after first occurrence of distant metastases; cohort B), and 14 healthy controls. Circulating antigen-reactive T cells were characterized by intracellular cytokine staining after in vitro stimulation. In cohort A patients, the presence of T cells responding to peptides from NY-ESO-1, Melan-A, or MAGE-3 and the M category according to the American Joint Committee on Cancer classification were significantly associated with survival. T cells responding to NY-ESO-1 and Melan-A (hazard ratios, 0.29 and 0.18, respectively) remained independent prognostic factors in Cox regression analysis and were superior to the M category in predicting outcome. Median survival of patients possessing T cells responding to NY-ESO-1, Melan-A, or both was 21 months, compared with 6 months for all others. NY-ESO-1-responsive T cells were detected in 70% of cohort A patients surviving > 18 months and in 50% of cohort B patients. Melan-A responses were found in 42% and 47% of patients in cohorts A and B, respectively. In contrast, the proportion was only 22% for NY-ESO-1 and 23% for Melan-A in those who died within 6 months. The presence of circulating T cells responding to Melan-A or NY-ESO-1 had strong independent prognostic impact on survival in advanced melanoma. Our findings support the therapeutic relevance of Melan-A and NY-ESO-1 as targets for immunotherapy.

  19. Tumor-reactive CD8+ T-cell responses after vaccination with NY-ESO-1 peptide, CpG 7909 and Montanide ISA-51: association with survival.

    Science.gov (United States)

    Karbach, Julia; Gnjatic, Sacha; Bender, Armin; Neumann, Antje; Weidmann, Eckhart; Yuan, Jianda; Ferrara, Cathy A; Hoffmann, Eric; Old, Lloyd J; Altorki, Nasser K; Jäger, Elke

    2010-02-15

    Peptide-based vaccines have led to the induction of antigen-specific CD8(+) T-cell responses in patients with NY-ESO-1 positive cancers. However, vaccine-induced T-cell responses did not generally correlate with improved survival. Therefore, we tested whether a synthetic CpG 7909 ODN (deoxycytidyl-deoxyguanosin oligodeoxy-nucleotides) mixed with NY-ESO-1 peptide p157-165 and incomplete Freund's adjuvants (Montanide(R) ISA-51) led to enhanced NY-ESO-1 antigen-specific CD8(+) immune responses in patients with NY-ESO-1 or LAGE-1 expressing tumors. Of 14 HLA-A2+ patients enrolled in the study, 5 patients withdrew prematurely because of progressive disease and 9 patients completed 1 cycle of immunization. Nine of 14 patients developed measurable and sustained antigen-specific CD8(+) T-cell responses: Four had detectable CD8+ T-cells against NY-ESO-1 after only 2 vaccinations, whereas 5 patients showed a late-onset but durable induction of NY-ESO-1 p157-165 specific T-cell response during continued vaccination after 4 months. In 6 patients, vaccine-induced antigen-specific T-cells became detectable ex vivo and reached frequencies of up to 0.16 % of all circulating CD8(+) T-cells. Postvaccine T-cell clones were shown to recognize and lyse NY-ESO-1 expressing tumor cell lines in vitro. In 6 of 9 patients developing NY-ESO-1-specific immune responses, a favorable clinical outcome with overall survival times of 43+, 42+, 42+, 39+, 36+ and 27+ months, respectively, was observed.

  20. Tumor-infiltrating NY-ESO-1-specific CD8+ T cells are negatively regulated by LAG-3 and PD-1 in human ovarian cancer.

    Science.gov (United States)

    Matsuzaki, Junko; Gnjatic, Sacha; Mhawech-Fauceglia, Paulette; Beck, Amy; Miller, Austin; Tsuji, Takemasa; Eppolito, Cheryl; Qian, Feng; Lele, Shashikant; Shrikant, Protul; Old, Lloyd J; Odunsi, Kunle

    2010-04-27

    NY-ESO-1 is a "cancer-testis" antigen frequently expressed in epithelial ovarian cancer (EOC) and is among the most immunogenic tumor antigens defined to date. In an effort to understand in vivo tolerance mechanisms, we assessed the phenotype and function of NY-ESO-1-specific CD8(+) T cells derived from peripheral blood lymphocytes (PBLs), tumor-infiltrating lymphocytes (TILs), and tumor-associated lymphocytes (TALs) of EOC patients with NY-ESO-1-expressing tumors, with or without humoral immunity to NY-ESO-1. Whereas NY-ESO-1-specific CD8(+) T cells were readily detectable ex vivo with tetramers in TILs and TALs of seropositive patients, they were only detectable in PBLs following in vitro stimulation. Compared with PBLs, tumor-derived NY-ESO-1-specific CD8(+) T cells demonstrated impaired effector function, preferential usage of dominant T-cell receptor, and enriched coexpression of inhibitory molecules LAG-3 and PD-1. Expression of LAG-3 and PD-1 on CD8(+) T cells was up-regulated by IL-10, IL-6 (cytokines found in tumor ascites), and tumor-derived antigen-presenting cells. Functionally, CD8(+)LAG-3(+)PD-1(+) T cells were more impaired in IFN-gamma/TNF-alpha production compared with LAG-3(+)PD-1(-) or LAG-3(-)PD-1(-) subsets. Dual blockade of LAG-3 and PD-1 during T-cell priming efficiently augmented proliferation and cytokine production by NY-ESO-1-specific CD8(+) T cells, indicating that antitumor function of NY-ESO-1-specific CD8(+) T cells could potentially be improved by therapeutic targeting of these inhibitory receptors.

  1. Synergetic effect of TeMo5O16 and MoO3 phases in MoTeOx catalysts used for the partial oxidation of propylene

    Institute of Scientific and Technical Information of China (English)

    Yiming He; Ying Wu; Weizheng Weng; Huilin Wan

    2011-01-01

    A detailed study on the synergetic effect of TeMo5O16 and MoO3 phases in the MoTeOx catalysts for the partial oxidation of propylene to acrolein has been reported in this work. It was found that both propylene conversion and acrolein selectivity increased with the addition of MoO3 to TeMo5O16. Based on the results of N2 adsorption-desorption, XRD, XPS, in-situ XRD, O2-TPO, and 2-propanol decomposition reaction, the higher catalytic performance and synergetic effect could be attributed to the enhancement of acidity and the oxygen transfer from TeMo5O16 to MoO3 phase.

  2. Elastic modulus of phases in Ti–Mo alloys

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Wei-dong [State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083 (China); Liu, Yong, E-mail: yonliu11@aliyun.com [State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083 (China); Wu, Hong; Song, Min [State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083 (China); Zhang, Tuo-yang [Metallurgical Engineering, University of Utah, Salt Lake City, UT 84112 (United States); Lan, Xiao-dong; Yao, Tian-hang [State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan 410083 (China)

    2015-08-15

    In this work, a series of binary Ti–Mo alloys with the Mo contents ranging from 3.2 to 12 at.% were prepared using non-consumable arc melting. The microstructures were investigated by X-ray diffraction and transmission electron microscope, and the elastic modulus was evaluated by nanoindentation testing technique. The evolution of the volume fractions of ω phase was investigated using X-ray photoelectron spectroscopy. The results indicated that the phase constitution and elastic modulus of the Ti–Mo alloys are sensitive to the Mo content. Ti–3.2Mo and Ti–8Mo alloys containing only α and β phases, respectively, have a low elastic modulus. In contrast, Ti–4.5Mo, Ti–6Mo, Ti–7Mo alloys, with different contents of ω phase, have a high elastic modulus. A simple micromechanical model was used to calculate the elastic modulus of ω phase (E{sub ω}), which was determined to be 174.354 GPa. - Highlights: • Ti–Mo alloys with the Mo contents ranging from 3.2 to 12 at.% were investigated. • XPS was used to investigate the volume fractions of ω phase. • The elastic modulus of Ti–Mo alloys is sensitive to the Mo content. • The elastic modulus of ω phase was determined to be 174.354 GPa.

  3. States of Carbon Nanotube Supported Mo-Based HDS Catalysts

    Institute of Scientific and Technical Information of China (English)

    Hongyan Shang; Chenguang Liu; Yongqiang Xu; Jieshan Qiu; Fei Wei

    2006-01-01

    The dispersion of the active phase and loading capacity of the Mo species on carbon nanotube (CNT) was studied by the XRD technique. The reducibility properties of Co-Mo catalysts in the oxide state over CNTs were investigated by TPR, while the sulfided Co-Mo/CNT catalysts were characterized by means of the XRD and LRS techniques. The activity and selectivity with respect to the hydrodesulfurization (HDS) performances on carbon nanotube supported Co-Mo catalysts were evaluated. It was found that the main active molybdenum species in the oxide state MoO3/CNT catalysts were MoO2, but not MoO3, as generally expected. The maximum loading before the formation of the bulk phase was lower than 6% (percent by mass, based on MoO3). TPR studies revealed that the active species in the oxide state Co-Mo/CNT catalysts were reduced more easily at relatively lower temperatures in comparison to those of the Co-Mo/γ-Al2O3 catalysts, indicating that the CNT support promoted or favored the reduction of the active species. The active species of a Co-Mo-0.7/CNT catalyst were more easily reduced than those of the Co-Mo/CNT catalysts with Co/Mo atomic ratios of 0.2, 0.35, and 0.5, respectively, suggesting that the Co/Mo atomic ratio has a great effect on the reducibility of the active species. It was found that the incorporation of cobalt improved the dispersion of the molybdenum species on the support, and a phenomenon of mobilization and re-dispersion had occurred during the sulfurization process, resulting in low valence state Mo3S4 and Co-MoS2.17 active phases. HDS measurements showed that the Co-Mo/CNT catalysts were more active than the Co-Mo/γ-Al2O3 ones for the desulfurization of DBT, and the hydrogenolysis/hydrogenation selectivity of the Co-Mo/CNT catalysts was also much higher than those of the Co-Mo/γ-Al2O3. The Co-Mo/CNT catalyst with a Co/Mo atomic ratio of 0.7 showed the highest activity, whereas the catalyst with a Co/Mo atomic ratio of 0.35 had the highest selectivity.

  4. Effect of MoO3 constituents on the growth of MoS2 nanosheets by chemical vapor deposition

    Science.gov (United States)

    Wang, Xuan; Zhang, Yong Ping; Qian Chen, Zhi

    2016-06-01

    The highly crystalline and uniform MoS2 film was grown on Si substrate by a low-pressure chemical vapor deposition method using S and MoO3 as precursors at an elevated temperature. The structures and properties of MoS2 nanosheets vary greatly with the content of MoO3 constituents in the films. The nanostructured MoS2 film exhibits strong photoluminescence in the visible range. This work may provide a pathway to synthesizing MoS2 nanosheets and facilitate the development of applicable devices.

  5. MoS2 PARTICLES MODIFIED WITH POLYSTYRENE FOR PRODUCING Ni–PS/MoS2 COATINGS

    OpenAIRE

    ZHONGJIA HUANG; DANGSHENG XIONG

    2008-01-01

    The MoS2 particles were coated with polystyrene and can be written as PS/MoS2 hereinafter. Ni–PS/MoS2 coatings and Ni–MoS2 coatings were produced by PC electrodeposition technique. The surface morphology of Ni–PS/MoS2 coating was examined and compared with those of Ni–MoS2 coating. The effect of particle concentrations on the volume percent of particles incorporated in the coatings was investigated. And the microhardness of coatings was also investigated. Results show that the surface morphol...

  6. Immunohistochemical expression of tumor antigens MAGE-A3/4 and NY-ESO-1 in renal oncocytoma and chromophobe renal cell carcinoma.

    Science.gov (United States)

    Demirović, Alma; Džombeta, Tihana; Tomas, Davor; Spajić, Borislav; Pavić, Ivana; Hudolin, Tvrtko; Milošević, Milan; Cupić, Hrvoje; Krušlin, Božo

    2010-10-15

    The distinction between renal oncocytoma (RO) and chromophobe renal cell carcinoma (ChRCC), especially the eosinophilic variant, can often be difficult. Our study has documented for the first time the expression of MAGE-A3/4 and NY-ESO-1 cancer testis antigens (CTAs) in these tumors. A total of 35 patients (17 ROs and 18 ChRCCs) were included in the study. Two antibodies were used for immunohistochemical staining: 57B recognizing multiple MAGE-A and D8.38 recognizing NY-ESO-1 CTAs. Fifteen (88.2%) samples of RO stained positively for both MAGE-A3/4 and NY-ESO-1 antigens. Regarding ChRCC, seven (38.9%) stained positively for MAGE-A3/4 and six (33.3%) for NY-ESO-1 antigens. Median MAGE-A3/4 expression was moderately positive in RO and negative in ChRCC. The difference in MAGE-A3/4 expression between two tumor groups was significant (P=0.0013). Median NY-ESO-1 expression was strongly positive in RO and negative in ChRCC. The difference in NY-ESO-1 expression between two tumor groups was also significant (P=0.0008). Our study has shown that RO had a significantly higher expression of both CTAs. However, additional research is needed to clarify their potential diagnostic implications.

  7. Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells.

    Science.gov (United States)

    Pagotto, Anna; Caballero, Otavia L; Volkmar, Norbert; Devalle, Sylvie; Simpson, Andrew J G; Lu, Xin; Christianson, John C

    2013-01-01

    The Cancer/Testis (CT) antigen family of genes are transcriptionally repressed in most human tissues but are atypically re-expressed in many malignant tumour types. Their restricted expression profile makes CT antigens ideal targets for cancer immunotherapy. As little is known about whether CT antigens may be regulated by post-translational processing, we investigated the mechanisms governing degradation of NY-ESO-1 and MAGE-C1 in selected cancer cell lines. Inhibitors of proteasome-mediated degradation induced the partitioning of NY-ESO-1 and MAGE-C1 into a detergent insoluble fraction. Moreover, this treatment also resulted in increased localisation of NY-ESO-1 and MAGE-C1 at the centrosome. Despite their interaction, relocation of either NY-ESO-1 or MAGE-C1 to the centrosome could occur independently of each other. Using a series of truncated fragments, the regions corresponding to NY-ESO-1(91-150) and MAGE-C1(900-1116) were established as important for controlling both stability and localisation of these CT antigens. Our findings demonstrate that the steady state levels of NY-ESO-1 and MAGE-C1 are regulated by proteasomal degradation and that both behave as aggregation-prone proteins upon accumulation. With proteasome inhibitors being increasingly used as front-line treatment in cancer, these data raise issues about CT antigen processing for antigenic presentation and therefore immunogenicity in cancer patients.

  8. Teens join the MoEDAL collaboration

    CERN Multimedia

    Stephanie Hills

    2013-01-01

    The principal investigator for any institute joining an experimental collaboration is generally a self-assured researcher with evident leadership skills and an in-depth knowledge of their subject gained over many years. Katherine Evans fits the brief in every respect, except that she is 17 years old and her research institute is the Langton Star Centre, based at the Simon Langton Grammar School for Boys. The school has just joined the MoEDAL experiment.   Teacher Becky Parker (left) with two students from the Simon Langton Grammar School for Boys in the MoEDAL experimental area. MoEDAL, the latest LHC experiment has detectors located close to the interaction point of the LHCb experiment. This new experiment is designed to search for the highly ionizing avatars of new physics at the LHC, specifically the magnetic monopole or dyon and other highly ionizing stable massive particles from a number of beyond-the-Standard-Model scenarios. MoEDAL was approved in 2010 and is due to start taking data i...

  9. MoSe2 / Polyaniline Solar Cells

    Directory of Open Access Journals (Sweden)

    H.S. Patel

    2011-01-01

    Full Text Available Solar cells have been investigated since long for harnessing the solar energy. During this decade, a new direction has come up where in the polymers have been used in the fabrication of solar cells. Polyaniline is one of the polymers which has shown potential for its applications in heterostructure solar cells. This material is being used along with the semiconductors like InSe, TiO2, Si etc. to form the photosensitive interface. In this direction, we report our investigations on the use of Molybdenum diselenide (MoSe2 as photosensitive semiconducting material in MoSe2 / polyaniline solar cells. In this paper, the preparation of MoSe2 / polyaniline solar cells has been reported. Also, the photovoltage → photocurrent characteristics of this structure have been discussed in detail in this paper. The variation of different parameters of MoSe2 / polyaniline solar cells (like open circuit voltage, short circuit current, photoconversion efficiency and fill factor with the intensity of incident illuminations has been reported in this paper. In present case, the photocurrent density was found to be around 250 µA/cm2 with the photovoltage around 8.5 mV (which is low the photoconversion efficiency was found to be around 0.7 % along with the fill factor around 0.33. The efforts have been made to explain the low values of the photoconversion efficiency.

  10. Accelerator Production Options for 99MO

    Energy Technology Data Exchange (ETDEWEB)

    Bertsche, Kirk; /SLAC

    2010-08-25

    Shortages of {sup 99}Mo, the most commonly used diagnostic medical isotope, have caused great concern and have prompted numerous suggestions for alternate production methods. A wide variety of accelerator-based approaches have been suggested. In this paper we survey and compare the various accelerator-based approaches.

  11. 78 FR 48762 - Missouri Disaster #MO-00065

    Science.gov (United States)

    2013-08-09

    ... ADMINISTRATION Missouri Disaster MO-00065 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY.... ADDRESSES: Submit completed loan applications to: U.S. Small Business Administration, Processing and..., Office of Disaster Assistance, U.S. Small Business Administration, 409 3rd Street SW., Suite...

  12. Hydrogen intercalation in MoS2

    Science.gov (United States)

    Zhu, Zhen; Peelaers, Hartwin; Van de Walle, Chris G.

    2016-08-01

    We investigate the structure and energetics of interstitial hydrogen and hydrogen molecules in layered 2 H -MoS2, an issue of interest both for hydrogen storage applications and for the use of MoS2 as an (opto)electronic material. Using first-principles density functional theory we find that hydrogen interstitials are deep donors. H2 molecules are electrically inactive and energetically more stable than hydrogen interstitials. Their equilibrium position is the hollow site of the MoS2 layers. The migration barrier of a hydrogen molecule is calculated to be smaller than 0.6 eV. We have also explored the insertion energies of hydrogen molecules as a function of hydrogen concentration in MoS2. For low concentrations, additional inserted H2 molecules prefer to be located in hollow sites (on top of the center of a hexagon) in the vicinity of an occupied site. Once two molecules have been inserted, the energy cost for inserting additional H2 molecules becomes much lower. Once all hollow sites are filled, the energy cost increases, but only by a modest amount. We find that up to 13 H2 molecules can be accommodated within the same interlayer spacing of an areal 3 ×3 supercell.

  13. MoEDAL AND MAGNETIC MONOPOLES

    CERN Multimedia

    Paola Catapano

    2016-01-01

    The LHC MoEDAL experiment publishes its first paper on its search for magnetic monopoles Geneva XXXX. In a paper published by the journal JHEP today, the MoEDAL experiment at CERN narrows the window of where to search for a hypothetical particle, the magnetic monopole. Over the last decades, experiments have been trying to find evidence for magnetic monopoles at accelerators, including at CERN’s Large Hadron Collider. Such particles were first predicted by physicist Paul Dirac in the 1930s but have never been observed so far. “Today MoEDAL celebrates the release of its first physics result and joins the other LHC experiments at the discovery frontier," says Spokesperson of the MoEDAL experiment, James Pinfold. Just as electricity comes with two charges, positive and negative, so magnetism comes with two poles, North and South. The difference is that while it’s easy to isolate a positive or negative electric charge, nobody has ever seen a solitary magnetic charge, or monopole. If you

  14. Measurement of the direct CP -violating parameter ACP in the decay D<mo>+ stretchy='false'>→mo>K<mo>-mo>π<mo>+mo>π+>

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.; Yin, H.; Yip, K.; Youn, S. W.; Yu, J. M.; Zennamo, J.; Zhao, T. G.; Zhou, B.; Zhu, J.; Zielinski, M.; Zieminska, D.; Zivkovic, L.

    2014-12-01

    We measure the direct CP-violating parameter ACP for the decay of the charged charm meson, Dmo>+ stretchy="false">→mo>Kmo>-mo>πmo>+mo>πmo>+> (and charge conjugate), using the full 10.4 fbmo>->1 sample of ppmo accent="true" stretchy="false">¯mo> collisions at smo>=>1.96 TeV collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from Bmo stretchy="false">→mo

  15. Measurement of the direct CP -violating parameter ACP in the decay D<mo>+ stretchy='false'>→mo>K<mo>-mo>π<mo>+mo>π+>

    Energy Technology Data Exchange (ETDEWEB)

    Abazov, V. M.; Abbott, B.; Acharya, B. S.; Adams, M.; Adams, T.; Agnew, J. P.; Alexeev, G. D.; Alkhazov, G.; Alton, A.; Askew, A.; Atkins, S.; Augsten, K.; Avila, C.; Badaud, F.; Bagby, L.; Baldin, B.; Bandurin, D. V.; Banerjee, S.; Barberis, E.; Baringer, P.; Bartlett, J. F.; Bassler, U.; Bazterra, V.; Bean, A.; Begalli, M.; Bellantoni, L.; Beri, S. B.; Bernardi, G.; Bernhard, R.; Bertram, I.; Besançon, M.; Beuselinck, R.; Bhat, P. C.; Bhatia, S.; Bhatnagar, V.; Blazey, G.; Blessing, S.; Bloom, K.; Boehnlein, A.; Boline, D.; Boos, E. E.; Borissov, G.; Borysova, M.; Brandt, A.; Brandt, O.; Brock, R.; Bross, A.; Brown, D.; Bu, X. B.; Buehler, M.; Buescher, V.; Bunichev, V.; Burdin, S.; Buszello, C. P.; Camacho-Pérez, E.; Casey, B. C. K.; Castilla-Valdez, H.; Caughron, S.; Chakrabarti, S.; Chan, K. M.; Chandra, A.; Chapon, E.; Chen, G.; Cho, S. W.; Choi, S.; Choudhary, B.; Cihangir, S.; Claes, D.; Clutter, J.; Cooke, M.; Cooper, W. E.; Corcoran, M.; Couderc, F.; Cousinou, M. -C.; Cutts, D.; Das, A.; Davies, G.; de Jong, S. J.; De La Cruz-Burelo, E.; Déliot, F.; Demina, R.; Denisov, D.; Denisov, S. P.; Desai, S.; Deterre, C.; DeVaughan, K.; Diehl, H. T.; Diesburg, M.; Ding, P. F.; Dominguez, A.; Dubey, A.; Dudko, L. V.; Duperrin, A.; Dutt, S.; Eads, M.; Edmunds, D.; Ellison, J.; Elvira, V. D.; Enari, Y.; Evans, H.; Evdokimov, V. N.; Fauré, A.; Feng, L.; Ferbel, T.; Fiedler, F.; Filthaut, F.; Fisher, W.; Fisk, H. E.; Fortner, M.; Fox, H.; Fuess, S.; Garbincius, P. H.; Garcia-Bellido, A.; García-González, J. A.; Gavrilov, V.; Geng, W.; Gerber, C. E.; Gershtein, Y.; Ginther, G.; Gogota, O.; Golovanov, G.; Grannis, P. D.; Greder, S.; Greenlee, H.; Grenier, G.; Gris, Ph.; Grivaz, J. -F.; Grohsjean, A.; Grünendahl, S.; Grünewald, M. W.; Guillemin, T.; Gutierrez, G.; Gutierrez, P.; Haley, J.; Han, L.; Harder, K.; Harel, A.; Hauptman, J. M.; Hays, J.; Head, T.; Hebbeker, T.; Hedin, D.; Hegab, H.; Heinson, A. P.; Heintz, U.; Hensel, C.; Heredia-De La Cruz, I.; Herner, K.; Hesketh, G.; Hildreth, M. D.; Hirosky, R.; Hoang, T.; Hobbs, J. D.; Hoeneisen, B.; Hogan, J.; Hohlfeld, M.; Holzbauer, J. L.; Howley, I.; Hubacek, Z.; Hynek, V.; Iashvili, I.; Ilchenko, Y.; Illingworth, R.; Ito, A. S.; Jabeen, S.; Jaffré, M.; Jayasinghe, A.; Jeong, M. S.; Jesik, R.; Jiang, P.; Johns, K.; Johnson, E.; Johnson, M.; Jonckheere, A.; Jonsson, P.; Joshi, J.; Jung, A. W.; Juste, A.; Kajfasz, E.; Karmanov, D.; Katsanos, I.; Kaur, M.; Kehoe, R.; Kermiche, S.; Khalatyan, N.; Khanov, A.; Kharchilava, A.; Kharzheev, Y. N.; Kiselevich, I.; Kohli, J. M.; Kozelov, A. V.; Kraus, J.; Kumar, A.; Kupco, A.; Kurča, T.; Kuzmin, V. A.; Lammers, S.; Lebrun, P.; Lee, H. S.; Lee, S. W.; Lee, W. M.; Lei, X.; Lellouch, J.; Li, D.; Li, H.; Li, L.; Li, Q. Z.; Lim, J. K.; Lincoln, D.; Linnemann, J.; Lipaev, V. V.; Lipton, R.; Liu, H.; Liu, Y.; Lobodenko, A.; Lokajicek, M.; Lopes de Sa, R.; Luna-Garcia, R.; Lyon, A. L.; Maciel, A. K. A.; Madar, R.; Magaña-Villalba, R.; Malik, S.; Malyshev, V. L.; Mansour, J.; Martínez-Ortega, J.; McCarthy, R.; McGivern, C. L.; Meijer, M. M.; Melnitchouk, A.; Menezes, D.; Mercadante, P. G.; Merkin, M.; Meyer, A.; Meyer, J.; Miconi, F.; Mondal, N. K.; Mulhearn, M.; Nagy, E.; Narain, M.; Nayyar, R.; Neal, H. A.; Negret, J. P.; Neustroev, P.; Nguyen, H. T.; Nunnemann, T.; Orduna, J.; Osman, N.; Osta, J.; Pal, A.; Parashar, N.; Parihar, V.; Park, S. K.; Partridge, R.; Parua, N.; Patwa, A.; Penning, B.; Perfilov, M.; Peters, Y.; Petridis, K.; Petrillo, G.; Pétroff, P.; Pleier, M. -A.; Podstavkov, V. M.; Popov, A. V.; Prewitt, M.; Price, D.; Prokopenko, N.; Qian, J.; Quadt, A.; Quinn, B.; Ratoff, P. N.; Razumov, I.; Ripp-Baudot, I.; Rizatdinova, F.; Rominsky, M.; Ross, A.; Royon, C.; Rubinov, P.; Ruchti, R.; Sajot, G.; Sánchez-Hernández, A.; Sanders, M. P.; Santos, A. S.; Savage, G.; Savitskyi, M.; Sawyer, L.; Scanlon, T.; Schamberger, R. D.; Scheglov, Y.; Schellman, H.; Schwanenberger, C.; Schwienhorst, R.; Sekaric, J.; Severini, H.; Shabalina, E.; Shary, V.; Shaw, S.; Shchukin, A. A.; Simak, V.; Skubic, P.; Slattery, P.; Smirnov, D.; Snow, G. R.; Snow, J.; Snyder, S.; Söldner-Rembold, S.; Sonnenschein, L.; Soustruznik, K.; Stark, J.; Stoyanova, D. A.; Strauss, M.; Suter, L.; Svoisky, P.; Titov, M.; Tokmenin, V. V.; Tsai, Y. -T.; Tsybychev, D.; Tuchming, B.; Tully, C.; Uvarov, L.; Uvarov, S.; Uzunyan, S.; Van Kooten, R.; van Leeuwen, W. M.; Varelas, N.; Varnes, E. W.; Vasilyev, I. A.; Verkheev, A. Y.; Vertogradov, L. S.; Verzocchi, M.; Vesterinen, M.; Vilanova, D.; Vokac, P.; Wahl, H. D.; Wang, M. H. L. S.; Warchol, J.; Watts, G.; Wayne, M.; Weichert, J.; Welty-Rieger, L.; Williams, M. R. J.; Wilson, G. W.; Wobisch, M.; Wood, D. R.; Wyatt, T. R.; Xie, Y.; Yamada, R.; Yang, S.; Yasuda, T.; Yatsunenko, Y. A.; Ye, W.; Ye, Z.

    2014-12-01

    We measure the direct CP-violating parameter ACP for the decay of the charged charm meson, Dmo>+ stretchy="false">→mo>Kmo>-mo>πmo>+mo>πmo>+> (and charge conjugate), using the full 10.4 fbmo>->1 sample of ppmo accent="true" stretchy="false">¯mo> collisions at smo>=>1.96 TeV collected by the D0 detector at the Fermilab Tevatron collider. We extract the raw reconstructed charge asymmetry by fitting the invariant mass distributions for the sum and difference of charge-specific samples. This quantity is then corrected for detector-related asymmetries using data-driven methods and for possible physics asymmetries (from Bmo stretchy="false">→mo

  16. Road and Street Centerlines - MO 2009 August MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT travelways coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple...

  17. Road and Street Centerlines - MO 2009 March MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  18. Road and Street Centerlines - MO 2008 August MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  19. Road and Street Centerlines - MO 2009 January MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  20. Road and Street Centerlines - MO 2008 August MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  1. Road and Street Centerlines - MO 2004 MoDOT Roads (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  2. Road and Street Centerlines - MO 2009 June MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  3. β-K3Fe(MoO42Mo2O7

    Directory of Open Access Journals (Sweden)

    Amira Souilem

    2014-07-01

    Full Text Available The title compound, tripotassium iron(III bis(orthomolybdate dimolybdate, was obtained by a solid-state reaction. The main structural building units are one FeO6 octahedron, two MoO4 tetrahedra and one Mo2O7 dimolybdate group, all with point group symmetries m. These units are linked via corner-sharing to form ribbons parallel to [010]. The three K+ cations are located between the ribbons on mirror planes and have coordination numbers of 10 and 12. Two O atoms of one of the MoO4 tetrahedra of the dimolybdate group are disordered over two positions in a 0.524 (11:0.476 (11 ratio. The structure of the title compound is compared briefly with that of Rb3FeMo4O15.

  4. Road and Street Centerlines - MO 2009 March MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  5. Road and Street Centerlines - MO 2008 November MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  6. Road and Street Centerlines - MO 2008 June MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  7. Road and Street Centerlines - MO 2009 June MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  8. Road and Street Centerlines - MO 2008 June MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  9. Road and Street Centerlines - MO 2009 January MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  10. Road and Street Centerlines - MO 2009 August MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT travelways coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple...

  11. Road and Street Centerlines - MO 2008 November MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  12. Road and Street Centerlines - MO 2008 July MoDOT Roads - Arcs (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  13. Road and Street Centerlines - MO 2007 July MoDOT Roads (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  14. Road and Street Centerlines - MO 2008 July MoDOT Roads - Routes (SHP)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The MoDOT roadway coverage is based on a Dynamic Segmentation model. Dynamic Segmentation models linear features using routes and events, associating multiple sets...

  15. Ground-based lidar measurements from Ny-Ålesund during ASTAR 2007

    Directory of Open Access Journals (Sweden)

    A. Herber

    2009-11-01

    Full Text Available During the Arctic Study of Tropospheric Aerosol, Clouds and Radiation (ASTAR in March and April 2007, measurements obtained at the AWIPEV Arctic Research Base in Ny-Ålesund, Spitsbergen at 78.9° N, 11.9° E (operated by the Alfred Wegener Institute for Polar and Marine Research – AWI and the Institut polaire français Paul-Emile Victor – IPEV, supported the airborne campaign. This included lidar data from the Koldewey Aerosol Raman Lidar (KARL and the Micro Pulse Lidar (MPL, located in the atmospheric observatory as well as photometer data and the daily launched radiosonde. The MPL features nearly continuous measurements; the KARL was switched on whenever weather conditions allowed observations (145 h in 61 days. From 1 March to 30 April, 71 meteorological balloon soundings were performed and compared with the concurrent MPL measurements; photometer measurements are available from 18 March. For the KARL data, a statistical overview of particle detection based on their optical properties backscatter ratio and volume depolarization can be given. The altitudes of the occurrence of the named features (subvisible and visible ice and water as well as mixed-phase clouds, aerosol layers as well as their dependence on different air mass origins are analyzed. Although the spring 2007 was characterized by rather clean conditions, diverse case studies of cloud and aerosol occurrence during March and April 2007 are presented in more detail, including temporal development and main optical properties as depolarization, backscatter and extinction coefficients. Links between air mass origins and optical properties can be presumed but need further evidence.

  16. Missing-Presumed Eroded: Holocene Sedimentation Patterns in Seneca Lake, NY

    Science.gov (United States)

    Loddengaard, K.; Curtin, T. M.

    2006-12-01

    The Finger Lakes of New York underwent a significant change in climate during the transition from the Hypsithermal to the Neoglacial at ~5 ka. To determine the effects of changes in climate on sedimentation patterns in the northern region of Seneca Lake, NY, we analyzed three cores that were collected along a shallow to deep water transect. Analyses of magnetic susceptibility, organic matter and carbonate content, fossil content, and grain size at a 1-cm interval were used to correlate cores, identify periods of erosion or non- deposition and assess the relative truncation of the Holocene sediment record. The stratigraphy and sedimentology of the cores indicate the lake contains a spatially and temporally discontinuous sediment record. Preliminary results indicate that the early to mid-Holocene sediment record was preferentially eroded in the deepwater cores, between 20 and 50 m water depth. At the deepwater sites, there is a gap in the sediment record that spans between ~12 and ~5 ka. Conversely, the late Holocene record is more complete in the deepwater cores than in the shallow water cores (12-20 m water depth). This data suggests a significant change on the controls and location of erosion and deposition in the lake during the Holocene. We hypothesize that the early to mid-Holocene sediment may have been eroded by strong internal seiches during stormy conditions that were initiated by cooler temperatures and a change in the position of the jet stream during the Hypsithermal-Neoglacial transition. Erosion occurred primarily in shallow water sites (<~30 m) during the Neoglacial, perhaps as a result of a change in the number and frequency of storms or strength of the internal seiche.

  17. Whole-lake nitrate addition for control of methylmercury in mercury-contaminated Onondaga Lake, NY.

    Science.gov (United States)

    Matthews, David A; Babcock, David B; Nolan, John G; Prestigiacomo, Anthony R; Effler, Steven W; Driscoll, Charles T; Todorova, Svetoslava G; Kuhr, Kenneth M

    2013-08-01

    Methylmercury (MeHg) strongly bioaccumulates in aquatic food webs resulting in exposure to humans and wildlife through consumption of fish. Production of MeHg is promoted by anaerobic conditions and the supply of inorganic Hg (Hg(2+)), sulfate (SO4(2-)), and labile organic carbon. The anaerobic sediments of stratified lakes are particularly active zones for methylation of Hg(2+) and can be an important source of MeHg to the water column during summer anoxia and fall turnover. Nitrate (NO3(-)) addition has recently been proposed as a novel approach for the control of MeHg accumulation in the hypolimnia of Hg-contaminated lakes. In 2011, a whole-lake NO3(-) addition pilot test was conducted in Hg-contaminated Onondaga Lake, NY with the objective of limiting release of MeHg from the pelagic sediments to the hypolimnion through maintenance of NO3(-)-N concentrations >1mgN/L. A liquid calcium-nitrate solution was added to the hypolimnion as a neutrally buoyant plume approximately three times per week during the summer stratification interval. Maximum hypolimnetic concentrations of MeHg and soluble reactive phosphorus (SRP) decreased 94% and 95% from 2009 levels, suggesting increased sorption to Fe and Mn oxyhydroxides in surficial sediments as the regulating mechanism. Increased MeHg concentrations in the upper waters during fall turnover, which had been a generally recurring pattern, did not occur in 2011, resulting in decreased exposure of aquatic organisms to MeHg. Over the 1992-2011 interval, the hypolimnetic NO3(-) supply explained 85% and 95% of the interannual variations in hypolimnetic accumulations of SRP and MeHg, respectively.

  18. Electron microscopy studies on MoS2 nanocrystals

    DEFF Research Database (Denmark)

    Hansen, Lars Pilsgaard

    Industrial-style MoS2-based hydrotreating catalysts are studied using electron microscopy. The MoS2 nanostructures are imaged with single-atom sensitivity to reveal the catalytically important edge structures. Furthermore, the in-situ formation of MoS2 crystals is imaged for the first time....

  19. Auger Spectroscopy Analysis of Spalled LEU-10Mo Foils

    Energy Technology Data Exchange (ETDEWEB)

    Lawrence, Samantha Kay [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Schulze, Roland K. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-08-03

    Presentation includes slides on Surface Science used to probe LEU-10Mo Spall; Auger highlights graphitic-like inclusions and Mo-deficient oxide on base metal; Higher C concentration detected within spall area Images Courtesy; Depth profiling reveals thick oxide; Mo concentration nears nominal only at depths ~400 nm; and lastly Key Findings.

  20. Electron microscopy studies on MoS2 nanocrystals

    DEFF Research Database (Denmark)

    Hansen, Lars Pilsgaard

    Industrial-style MoS2-based hydrotreating catalysts are studied using electron microscopy. The MoS2 nanostructures are imaged with single-atom sensitivity to reveal the catalytically important edge structures. Furthermore, the in-situ formation of MoS2 crystals is imaged for the first time....

  1. Influence of Mo on the Fe:Mo:C nanocatalyst thermodynamics for single-walled carbon nanotube growth

    Science.gov (United States)

    Curtarolo, Stefano; Awasthi, Neha; Setyawan, Wahyu; Jiang, Aiqin; Bolton, Kim; Tokune, Toshio; Harutyunyan, Avetik R.

    2008-08-01

    We explore the role of Mo in Fe:Mo nanocatalyst thermodynamics for low-temperature chemical-vapor deposition growth of single-walled carbon nanotubes (SWCNTs). By using the size-pressure approximation and ab initio modeling, we prove that for both Fe-rich ( ˜80% Fe or more) and Mo-rich ( ˜50% Mo or more) Fe:Mo clusters, the presence of carbon in the cluster causes nucleation of Mo2C . This enhances the activity of the particle since it releases Fe, which is initially bound in a stable Fe:Mo phase, so that it can catalyze SWCNT growth. Furthermore, the presence of small concentrations of Mo reduces the lower size limit of low-temperature steady-state growth from ˜0.58nm for pure Fe particles to ˜0.52nm . Our ab initio-thermodynamic modeling explains experimental results and establishes a direction to search for better catalysts.

  2. 胃癌中肿瘤/睾丸抗原的表达研究及NY-ESO-1蛋白的自身抗体检测%Analysis of CT antigen expression and humoral immunogenicity of NY-ESO-1 protein in gastric carcinoma

    Institute of Scientific and Technical Information of China (English)

    吴晓江; 王殮; 季加孚

    2005-01-01

    目的:分析胃癌中11种CT抗原(cancer/testis antigen)基因的表达分布及NY-ESO-1蛋白引发的自身体液免疫应答,为胃癌的特异性肿瘤疫苗治疗提供依据.方法:利用RT-PCR方法,检测101例胃癌患者肿瘤标本和对应正常胃黏膜中11种CT抗原基因的表达.ELISA方法检测抗NY-ESO-1抗体.利用NY-ESO-1的单抗E978,应用免疫组化方法检测组织切片中NY-ESO-1抗原物质.结果:101例胃癌患者中,74.3%的患者至少表达1种检测的CT抗原基因.12例患者表达NY-ESO-1 mRNA,5例可以检测到NY-ESO-1抗原蛋白以及针对NY-ESO-1抗原的体液免疫反应.结论:胃癌中表达多种CT抗原基因,表达率较高的MAGE-3,SSX-4和NY-ESO-1/LAGE-1蛋白等可作为肿瘤疫苗的备选抗原,其中NY-ESO-1蛋白的免疫原性得到验证.CT抗原的多价肿瘤疫苗在胃癌治疗中具有一定的可行性.

  3. Clinical implications of expression of MAGE-1 and NY-ESO-1 mRNAs in gastrointestinal stromal tumors%肿瘤-睾丸抗原MAGE-1和NY-ESO-1 mRNA在胃肠间质瘤中的表达

    Institute of Scientific and Technical Information of China (English)

    赖斌; 朱培谦; 朱胜昌; 罗洪亮

    2010-01-01

    目的:探讨肿瘤-睾丸抗原(CTA)MAGE-1和NY-ESO-1作为胃肠间质瘤(GISTs)免疫治疗特异性靶点及MAGE-1和NY-ESO-1 mRNA作为辅助GISTs危险度分级指标的可能性.及其与GISTs生物学行为的关系.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测30例GISTs中MAGE-1和NY-ESO-1mRNA的表达,并取正常胃肠道组织作为阴性对照组,同时分析MAGE-1和NY-ESO-1mRNA表达与病理特征的关系.结果:正常对照组中无阳性表达,30例GISTs中,18例至少表达一种CTA,MAGE-1和NY-ESO-1 mRNA在GISTs中的表达率分别为30%和47%.MAGE-1和NY-ESO-1 mRNA表达与患者年龄、性别和病理类型无关,而与肿瘤生长部位、肿瘤大小及危险度分级有关(P0.05).结论:MAGE-1和NY-ESO-1 mRNA在GISTs中的高特异性表达,其抗原有望成为GISTs免疫治疗特异性的靶点:MAGE-1和NY-ESO-1 mRNA表达与GISTs危险度分级有关,MAGE-1和NY-ESO-1 mRNA有望成为辅助GISTs危险度分级的诊断指标.

  4. A systematic first principle method to study magnetic properties of FeMo, CoMo and NiMo

    CERN Document Server

    Bhattacharjee, A K; Halder, A; Mookerjee, A; Touheed, M

    2003-01-01

    We use the first principle TB-LMTO (Tight-Binding Linear Muffin Tin Orbital) method combined with the ASM (Augmented Space Method) to take care of disorder beyond the mean field (CPA - Cohetent Potential Approximation) approximation. We analyze binary alloys between magnetic transition metals Fe, Co, Ni and non-magnetic Mo to find out the effect of disorder on electronic structure and consequently magnetic properties of the alloys.

  5. Influence of Pretreatment Conditions on Methane Aromatization Performance of Mo/HZSM-5 and Mo-Cu/HZSM-5 Catalysts

    Institute of Scientific and Technical Information of China (English)

    Yiping Zhang; Dongjie Wang; Jinhua Fei; Xiaoming Zheng

    2003-01-01

    Mo/HZSM-5 is a good catalyst for methane aromatization, and the reaction performance of Mo/HZSM-5 and Cu modified Mo/HZSM-5 catalysts under various pretreatment conditions has been studied. The results indicate that the catalyst presented a distinguished catalytic activity, benzene selectivity and a high stability when the bed temperature was raised in N2 atmosphere.

  6. Development of CaMoO4 crystal scintillators for double beta decay experiment with 100-Mo

    CERN Document Server

    Annenkov, A N; Danevich, F A; Georgadze, A S; Kim, S K; Kim, H J; Kim, Y D; Kobychev, V V; Kornoukhov, V N; Korzhik, M; Lee, J I; Missevitch, O; Mokina, V M; Nagorny, S S; Nikolaiko, A S; Poda, D V; Podviyanuk, R B; Sedlak, D J; Shkulkova, O G; So, J H; Solsky, I M; Tretyak, V I; Yurchenko, S S

    2007-01-01

    Energy resolution, alpha/beta ratio, pulse-shape discrimination for gamma rays and alpha particles, temperature dependence of scintillation properties, and radioactive contamination were studied with CaMoO4 crystal scintillators. A high sensitivity experiment to search for neutrinoless double beta decay of 100-Mo by using CaMoO4 scintillators is discussed.

  7. pRV-Display-NY-ESO-1真核表达质粒的构建及其在Myc-CaP细胞株中的稳定表达%Construction of pRV-Display-NY-ESO-1 eukaryotic plasmid and its stable transfected Myc-CaP cell line

    Institute of Scientific and Technical Information of China (English)

    谢冲; 王国民

    2014-01-01

    Objective To construct the pRV-Display-NY-ESO-1 eukaryotic plasmid,and establish Myc-CaP cell line transfected with NY-ESO-1.Methods The full sequence of NY-ESO-1 was get from Genbank and amplified by polymerase chain reaction (PCR).Insert the fragments into p-Display eukaryotic expression vector.The recombinant plasmid with targeted gene was identified by digestion.Display-NY-ESO-1 was amplified by PCR and inserted into pRV retroviral vector.Retrovirus was produced by Phoenix cell line,and transfected into Myc-CaP cell line.Results The recombinant plasmid pRV-Display-NY-ESO-1 was successfully constructed and identified by PCR and DNA sequencing.Green fluorescence was observed on Phoenix cells and Myc-CaP cells encoding with GFP.The purity of Myc-CaP cells encoded with NY-ESO-1 was more than 80% according to flow cytometry.Conclusion Myc-CaP cell line was stably transfected with pRV-Display-NY-ESO-1,which is the base for further experiments.%目的 构建pRV-Display-NY-ESO-1真核表达质粒,建立并检测稳定表达纽约人食管鳞状细胞癌抗原(NY-ESO-1)的Myc-CaP细胞株.方法 从Genbank查找NY-ESO-1基因全长序列,聚合酶链反应(PCR)扩增目的片段,克隆至pDisplay载体,构建pDisplay-NY-ESO-1真核表达质粒,双酶切证实NY-ESO-1插入后,PCR扩增并定向连入pRV载体,构建pRV-Display-NY-ESO-1真核表达质粒,同时构建pDisplay-绿色荧光蛋白(GFP)作为阳性对照.经酶切、测序鉴定后,用Phoenix细胞产生逆转录病毒,脂质体转染法将重组质粒转入Myc-CaP细胞,并用Western blot和流式细胞术鉴定稳定转染的Myc-CaP细胞中NY-ESO-1的表达.结果 经PCR、测序鉴定成功构建pRV-Display-NY-ESO-1真核重组质粒.表达GFP的Phoenix细胞和Myc-CaP细胞在倒置荧光显微镜下均可见绿色荧光.Western blot检测Myc-CaP细胞中NY-ESO-1表达阳性,流式细胞术鉴定携带目的基因的Myc-CaP细胞纯度均大于80%.结论 pRV-Display-NY-ESO-1真核重组质粒稳定高表达于Myc-CaP细胞.

  8. A trinuclear Mo-Fe-Mo cluster compound [ Et4N] {[Me2dtcMoO(μ-S) 2] 2Fe} containing dialkyldithiocarbamate ligand

    Institute of Scientific and Technical Information of China (English)

    ZHANG, Yan-Shi; LIU, Qiu-Tian; ZHU, Hong-Ping; CHEN, Yi-Hui; CHEN, Chang-Neng

    2000-01-01

    A trinuclear linear Mo-Fe-Mo dialkyldithiocarbamate complex [Et4N] {[Me2dtcMoO(μ-S)2]2Fe} has been obtained and structurally characterized, which contains two Me2dtcMoO(μ-S)2 units coordinated to a central tetrahedral Fe atom. A comparison of the structural parameters indicates the metal oxidation states of 2Mo(v) + Fe(Ⅲ). The 1H NMR shows chemical shifts of Me2dtc ligands at δ 10.14 and δ 9.40 with the intensity ratio of 1:1. The cyclic voltammogram displays a reversiible couple at - 1.41 V/- 1.36 V responsible for 1-/2-anions of the complex and an irreversible oxidation at 0.5 V,which seems to show the apparent lack of stability for its neutral species (Me2dtcMoOS2)2Fe.

  9. MAGEA4 expression in bone and soft tissue tumors: its utility as a target for immunotherapy and diagnostic marker combined with NY-ESO-1.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Ishii, Takeaki; Maekawa, Akira; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Matsumoto, Yoshihiro; Iwamoto, Yukihide; Oda, Yoshinao

    2017-07-26

    Cancer-testis (CT) antigens have promise as targets for immunotherapy, because of their restricted expression in tumor or testis tissue. MAGEA4 is both a MAGE family member and a CT antigen, and has attracted attention as a potential immunotherapeutic target. We investigated MAGEA4 expression by immunohistochemistry in bone and soft tissue tumor specimens that consisted of 35 malignant or intermediate and 24 benign histological subtypes, in order to evaluate its possible utility as an immunotherapy target and its potential use as a diagnostic marker when combined with another CT antigen, NY-ESO-1. Among these tumors, MAGEA4 was detected in 82.2% of synovial sarcomas, 67.7% of myxoid liposarcomas, 43.8% of osteosarcomas, 41.4% of angiosarcomas, 24.6% of malignant peripheral nerve sheath tumors (MPNSTs), and 21.4% of chondrosarcomas. NY-ESO-1 expression was found in 88.2% of myxoid liposarcomas, 61.1% of synovial sarcomas, 31.3% of osteosarcomas, 21.4% of pleomorphic liposarcomas, 16.7% of desmoplastic small round cell tumors, and 14.3% of chondrosarcomas. Benign tumors and non-tumorous tissue, except for testis tissue, did not express MAGEA4 or NY-ESO-1. Combined use of MAGEA4 and NY-ESO-1 increased the sensitivity, specificity, positive predictive values, and negative predictive values for distinguishing synovial sarcoma from spindle cell tumors and other mimicking tumors, compared to individual use of MAGEA4 or NY-ESO-1. Our results support the immunotherapy targeting MAGEA4 or NY-ESO-1 can be an ancillary therapy in the above-mentioned tumors, and the potential utility of MAGEA4 as an ancillary diagnostic marker for synovial sarcoma combined with NY-ESO-1.

  10. A pilot trial using lymphocytes genetically engineered with an NY-ESO-1-reactive T-cell receptor: long-term follow-up and correlates with response.

    Science.gov (United States)

    Robbins, Paul F; Kassim, Sadik H; Tran, Thai L N; Crystal, Jessica S; Morgan, Richard A; Feldman, Steven A; Yang, James C; Dudley, Mark E; Wunderlich, John R; Sherry, Richard M; Kammula, Udai S; Hughes, Marybeth S; Restifo, Nicholas P; Raffeld, Mark; Lee, Chyi-Chia R; Li, Yong F; El-Gamil, Mona; Rosenberg, Steven A

    2015-03-01

    Although adoptive cell therapy can be highly effective for the treatment of patients with melanoma, the application of this approach to the treatment of other solid tumors has been limited. The observation that the cancer germline (CG) antigen NY-ESO-1 is expressed in 70% to 80% and in approximately 25% of patients with synovial cell sarcoma and melanoma, respectively, prompted us to perform this first-in-man clinical trial using the adoptive transfer of autologous peripheral blood mononuclear cells that were retrovirally transduced with an NY-ESO-1-reactive T-cell receptor (TCR) to heavily pretreated patients bearing these metastatic cancers. HLA-*0201 patients with metastatic synovial cell sarcoma or melanoma refractory to standard treatments and whose cancers expressed NY-ESO-1 received autologous TCR-transduced T cells following a lymphodepleting preparative chemotherapy. Response rates using Response Evaluation Criteria in Solid Tumors (RECIST), as well as immunologic correlates of response, are presented in this report. Eleven of 18 patients with NY-ESO-1(+) synovial cell sarcomas (61%) and 11 of 20 patients with NY-ESO-1(+) melanomas (55%) who received autologous T cells transduced with an NY-ESO-1-reactive TCR demonstrated objective clinical responses. The estimated overall 3- and 5-year survival rates for patients with synovial cell sarcoma were 38% and 14%, respectively, whereas the corresponding estimated survival rates for patients with melanoma were both 33%. The adoptive transfer of autologous T cells transduced with a retrovirus encoding a TCR against an HLA-A*0201 restricted NY-ESO-1 epitope can be an effective therapy for some patients bearing synovial cell sarcomas and melanomas that are refractory to other treatments. ©2014 American Association for Cancer Research.

  11. NY-ESO-1 autoantibody as a tumor-specific biomarker for esophageal cancer: screening in 1969 patients with various cancers.

    Science.gov (United States)

    Oshima, Yoko; Shimada, Hideaki; Yajima, Satoshi; Nanami, Tatsuki; Matsushita, Kazuyuki; Nomura, Fumio; Kainuma, Osamu; Takiguchi, Nobuhiro; Soda, Hiroaki; Ueda, Takeshi; Iizasa, Toshihiko; Yamamoto, Naoto; Yamamoto, Hiroshi; Nagata, Matsuo; Yokoi, Sana; Tagawa, Masatoshi; Ohtsuka, Seiko; Kuwajima, Akiko; Murakami, Akihiro; Kaneko, Hironori

    2016-01-01

    Although serum NY-ESO-1 antibodies (s-NY-ESO-1-Abs) have been reported in patients with esophageal carcinoma, this assay system has not been used to study a large series of patients with various other cancers. Serum samples of 1969 cancer patients [esophageal cancer (n = 172), lung cancer (n = 269), hepatocellular carcinoma (n = 91), prostate cancer (n = 358), gastric cancer (n = 313), colorectal cancer (n = 262), breast cancer (n = 365)] and 74 healthy individuals were analyzed using an originally developed enzyme-linked immunosorbent assay system for s-NY-ESO-1-Abs. The optical density cut-off value, determined as the mean plus three standard deviations for serum samples from the healthy controls, was fixed at 0.165. Conventional tumor markers were also evaluated in patients with esophageal carcinoma. The positive rate of s-NY-ESO-1-Abs in patients with esophageal cancer (31 %) was significantly higher than that in the other groups: patients with lung cancer (13 %), patients with hepatocellular carcinoma (11 %), patients with prostate cancer (10 %), patients with gastric cancer (10 %), patients with colorectal cancer (8 %), patients with breast cancer (7 %), and healthy controls (0 %). The positive rate of s-NY-ESO-1-Abs was comparable to that of serum p53 antibodies (33 %), squamous cell carcinoma antigen (36 %), carcinoembryonic antigen (26 %), and CYFRA 21-1 (18 %) and gradually increased with the tumor stage. The positive rate of s-NY-ESO-1-Abs was significantly higher in patients with esophageal cancer than in patients with the other types of cancers. On the basis of its high specificity and sensitivity, even in patients with stage I tumors, s-NY-ESO-1-Abs may be one of the first choices for esophageal cancer.

  12. Prevalence of plasma autoantibody against cancer testis antigen NY-ESO-1 in HTLV-1 infected individuals with different clinical status.

    Science.gov (United States)

    Shiohama, Yasuo; Naito, Tadasuke; Matsuzaki, Toshio; Tanaka, Reiko; Tomoyose, Takeaki; Takashima, Hiroshi; Fukushima, Takuya; Tanaka, Yuetsu; Saito, Mineki

    2017-07-17

    Detection of specific immune responses against cancer/testis antigen NY-ESO-1 was recently reported in patients with adult T-cell leukemia/lymphoma (ATL) and human T-cell leukemia virus type 1 (HTLV-1)-infected asymptomatic carriers (ACs). However, the relationship of the responses with the HTLV-1 proviral load (PVL) and the levels of viral gene expression remain unclear. We measured plasma levels of autoantibodies to NY-ESO-1 immunogenic tumor antigen in HTLV-1-infected individuals with different clinical status, and in healthy controls. Data were compared to tax and HBZ mRNA levels, and PVL. Plasma anti-NY-ESO-1 antibody was detectable in 13.7% (7/51) of ACs, 29.2% (38/130) of patients with HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP), and 18.9% (10/53) of patients with ATL. Anti-NY-ESO-1 plasma levels were significantly higher in patients with HAM/TSP than in patients with ATL or ACs. Anti-NY-ESO-1 levels were not associated with PVL or the expression levels of tax and HBZ mRNA among HTLV-1-infected individuals, regardless of clinical status. The present results indicate the strong humoral immune response against NY-ESO-1 in natural HTLV-1 infection, irrespective of the clinical status. The higher immunoreactivity against NY-ESO-1 is not simply associated with the levels of both HTLV-1 gene expression and the number of infected cells in vivo. Rather, it might reflect chronic and generalized immune activation in infected individuals.

  13. Comparison of lithographic performance between MoSi binary mask and MoSi attenuated PSM

    Science.gov (United States)

    Yamana, Mitsuharu; Lamantia, Matthew; Philipsen, Vicky; Wada, Shingo; Nagatomo, Tatsuya; Tonooka, Yoji

    2009-04-01

    The mask error budget continues to shrink with shrinking DRAM half pitch and MPU gate size year by year. The ITRS roadmap calls for mask CDU to be cut in half by 2014[1]. Both mask maker and mask user must take advantage of various mask properties, OPC strategies and resolution enhancement techniques to drive improvements. Mask material selection impacts both lithographic performance and mask manufacturability. In turn mask material properties and manufacturing techniques impact our ability to meet the technology roadmap. Studies have shown the advantages of polarized light[2,3] as well as the impact of various mask materials on high NA lithography[4]. In this paper we select the recently introduced binary mask material made from a MoSi absorber called Opaque MoSi On Glass (OMOG) for comparison with the conventional 6% att. PSM and 20% att. MoSi PSM. Through simulation and wafer prints, we optimized mask feature from viewpoint of MEEF and maximum exposure latitude (EL). The MoSi att. PSMs suffer from higher MEEF, which is attributed to the negative effect of TE polarization for mask duty cycle of 50% for 50 nm half pitch and below. Therefore a lower mask duty cycle is required for att. PSM to bring the MEEF performance back to acceptable levels. Experimental results confirm simulation results. As a result of the lower mask duty cycle, the att. MoSi PSMs exhibit poor Sub Resolution Assist Feature (SRAF) printability. On the contrary, the MoSi binary mask delivers both acceptable MEEF and acceptable SRAF printing performance. Moreover, we found that the mask structure impact of OMOG to wafer CD is smallest among three masks. OMOG gives the best combination of lithographic performance and delivery compared to the MoSi att. PSMs.

  14. Level studies of 93Mo via 93Nb(p, nγ93Mo reaction and density of discrete levels in 93Mo

    Directory of Open Access Journals (Sweden)

    Razavi Rohallah

    2011-01-01

    Full Text Available The excited states of 93Mo have been investigated via the 93Nb(P,nγ93Mo reaction with proton beam energies of 2.5-4.3 MeV. The parameters of the nuclear level density formula were determined from the extensive and complete level scheme of 93Mo. The Bethe formula for the back-shifted Fermi gas model and the constant temperature model are compared with experimental level densities.

  15. Level structure of [sup 89]Mo

    Energy Technology Data Exchange (ETDEWEB)

    Garcia-Bermudez, G.; Cardona, M.A.; Ribas, R.V.; Filevich, A.; Achterberg, E.; Szybisz, L. (Laboratorio TANDAR, Departamento de Fisica, Comision Nacional de Energia Atomica, Av. del Libertador 8250, 429 Buenos Aires (Argentina))

    1993-10-01

    The level structure of [sup 89]Mo has been studied with the [sup 60]Ni([sup 32]S,2[ital pn]) reaction at 110 MeV beam energy. Mainly two bands of [gamma] rays depopulating states of probable spin-parity values ranging up to (27/2)[sup +] were determined from the excitation function, neutron and [gamma]-[gamma] coincidences, and [gamma]-ray angular distributions. The high-spin states of [sup 89]Mo show a scheme very similar to that of the [sup 87]Zr isotone. The interpretation of these states in terms of the three neutron-hole configuration ([nu][ital g][sub 9/2])[sup [minus]3] and core-excited states is discussed.

  16. Formation of Silicide Coating layer on U-Mo Powder

    Energy Technology Data Exchange (ETDEWEB)

    Nam, Ji Min; Kim, Sunghwan; Lee, Kyu Hong [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2014-05-15

    High-density U-Mo alloys are regarded as promising candidates for advanced research reactor fuel as they have shown stable irradiation performance when compared to other uranium alloys and compounds. However, interaction layer formation between the U-Mo alloys and Al matrix degrades the irradiation performance of U-Mo Dispersion fuel. Therefore, the addition of Ti in U-Mo alloys, the addition of Si in a Al matrix, and silicide or nitride coating on the surface of U-Mo particles have been proposed to inhibit the interaction layer growth. In this study, U-Mo alloy powder was produced using a centrifugal atomization method. In addition, silicide coating layers were fabricated by several mixing process changes on the surface of the U-Mo particles. The coated powders were characterized by using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDAX). Decreased annealing duration did not affect the forming of silicide coating layers on the surface of U-7wt%Mo powders. The variation in the mixing ratio between U-7wt%Mo and Si powders had an effect on the quality of silicide coating on the U-7wt%Mo powders. The weight of Si powders should be smaller than that of U-7wt%Mo powders for better silicide coating when it comes to the mixing ratio.

  17. National Dam Safety Program. Lake Wanda Dam (NJ00510), Hudson River Basin, Tributary to Wawayanda Creek, Sussex County, New Jersey. Phase 1 Inspection Report.

    Science.gov (United States)

    1980-03-01

    Cheke SQUARES 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 1/4 IN. SCALE 1 3 4 4= 2. P7 1,, 5PilW/V 9 10 12 13...191, ~ C! . .. .. C.C C C L. C . C W .C . . . .C .C . . . .C .C . . . . .C . C . C C. . C . C 4 4 . . . a CC aa acca a aa aa C C...4-9 - ---- 4. 4. CY C..L Q Z i m )4 %C .m 0 n o.C..Q J r o )%.? %c l r4 - a C. C’ C~~ C) C- C. C. CC - --- 04--. N Nl N% NY N N N N P7 ")7 F )7,F .F C

  18. Arctic warming, moisture increase and circulation changes observed in the Ny-Ålesund homogenized radiosonde record

    Science.gov (United States)

    Maturilli, Marion; Kayser, Markus

    2017-04-01

    Compared to global warming, the feedback mechanisms of Arctic Amplification lead to an increase of surface temperature in the Arctic by a factor of two. Yet, the vertical structure of Arctic warming and its resulting radiative feedbacks are poorly understood. Here, we focus on the analysis of the atmospheric column above Ny-Ålesund (78.9° N, 11.9° E), Svalbard. At Ny-Ålesund, radiosondes have been launched on a daily basis since 1993 in support of synoptic observations. The obtained radiosonde measurements 1993 to 2014 have been homogenized accounting for instrumentation discontinuities and known errors in the manufacturer provided profiles. From the homogenized data record, a first upper-air climatology of wind, humidity and temperature above Ny-Ålesund is presented, forming the background for the analysis of changes detected during the 22-year period. Particularly during the winter season, a strong increase in atmospheric humidity and temperature is observed, with a significant warming of the free troposphere in January and February of up to 3 K per decade. This winter warming is even more pronounced in the boundary layer below 1 km, presumably amplified by local conditions including e.g. orographic effects or the boundary layer capping inversion. Also the largest contribution to the increasing atmospheric water vapour column originates from the lowermost 2 km of the atmosphere where specific humidity inversions are frequently observed. Yet, no increase in the water vapour contribution by humidity inversions is detected. Instead, we find an increase in the humidity content of the large scale background humidity profiles to be responsible for the observed increase in winter integrated water vapour. The observed difference in the frequency occurrence of wind directions in the free troposphere between the first and second half of the 22-year period implies that the large scale synoptic flow over Svalbard has changed over the years. During the winter season, the

  19. Antibody Responses to NY-ESO-1 in Primary Breast Cancer Identify a Subtype Target for Immunotherapy

    OpenAIRE

    2011-01-01

    The highly immunogenic human tumor antigen NY-ESO-1 (ESO) is a target of choice for anti-cancer immune therapy. In this study, we assessed spontaneous antibody (Ab) responses to ESO in a large cohort of patients with primary breast cancer (BC) and addressed the correlation between the presence of anti-ESO Ab, the expression of ESO in the tumors and their characteristics. We found detectable Ab responses to ESO in 1% of the patients. Tumors from patients with circulating Ab to ESO exhibited co...

  20. Deoxygenation of glycolaldehyde and furfural on Mo2C/Mo(100)

    Science.gov (United States)

    McManus, Jesse R.; Vohs, John M.

    2014-12-01

    The desire to produce fuels and chemicals in an energy conscious, environmentally sympathetic approach has motivated considerable research on the use of cellulosic biomass feedstocks. One of the major challenges facing the utilization of biomass is finding effective catalysts for the efficient and selective removal of oxygen from the highly-oxygenated, biomass-derived platform molecules. Herein, a study of the reaction pathways for the biomass-derived platform molecule furfural and biomass-derived sugar model compound glycolaldehyde provides insight into the mechanisms of hydrodeoxygenation (HDO) on a model molybdenum carbide catalyst, Mo2C/Mo(100). Using temperature programmed desorption (TPD) and high resolution electron energy loss spectroscopy (HREELS), it was found that the Mo2C/Mo(100) catalyst was active for selective deoxygenation of the aldehyde carbonyl by facilitating adsorption of the aldehyde in an η2(C,O) bonding configuration. Furthermore, the catalyst showed no appreciable activity for furanic ring hydrogenation, highlighting the promise of relatively inexpensive Mo2C catalysts for selective HDO chemistry.