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Sample records for minichromosome maintenance protein

  1. Comprehensive Characterization of Minichromosome Maintenance Complex (MCM) Protein Interactions Using Affinity and Proximity Purifications Coupled to Mass Spectrometry.

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    Dubois, Marie-Line; Bastin, Charlotte; Lévesque, Dominique; Boisvert, François-Michel

    2016-09-02

    The extensive identification of protein-protein interactions under different conditions is an important challenge to understand the cellular functions of proteins. Here we use and compare different approaches including affinity purification and purification by proximity coupled to mass spectrometry to identify protein complexes. We explore the complete interactome of the minichromosome maintenance (MCM) complex by using both approaches for all of the different MCM proteins. Overall, our analysis identified unique and shared interaction partners and proteins enriched for distinct biological processes including DNA replication, DNA repair, and cell cycle regulation. Furthermore, we mapped the changes in protein interactions of the MCM complex in response to DNA damage, identifying a new role for this complex in DNA repair. In summary, we demonstrate the complementarity of these approaches for the characterization of protein interactions within the MCM complex.

  2. Structure of a double hexamer of the Pyrococcus furiosus minichromosome maintenance protein N-terminal domain

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    Meagher, Martin; Enemark, Eric J.

    2016-06-22

    The crystal structure of the N-terminal domain of thePyrococcus furiosusminichromosome maintenance (MCM) protein as a double hexamer is described. The MCM complex is a ring-shaped helicase that unwinds DNA at the replication fork of eukaryotes and archaea. Prior to replication initiation, the MCM complex assembles as an inactive double hexamer at specific sites of DNA. The presented structure is highly consistent with previous MCM double-hexamer structures and shows two MCM hexamers with a head-to-head interaction mediated by the N-terminal domain. Minor differences include a diminished head-to-head interaction and a slightly reduced inter-hexamer rotation.

  3. Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding

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    Brewster Aaron S

    2010-08-01

    Full Text Available Abstract Background The mini-chromosome maintenance protein (MCM complex is an essential replicative helicase for DNA replication in Archaea and Eukaryotes. While the eukaryotic complex consists of six homologous proteins (MCM2-7, the archaeon Sulfolobus solfataricus has only one MCM protein (ssoMCM, six subunits of which form a homohexamer. We have recently reported a 4.35Å crystal structure of the near full-length ssoMCM. The structure reveals a total of four β-hairpins per subunit, three of which are located within the main channel or side channels of the ssoMCM hexamer model generated based on the symmetry of the N-terminal Methanothermobacter thermautotrophicus (mtMCM structure. The fourth β-hairpin, however, is located on the exterior of the hexamer, near the exit of the putative side channels and next to the ATP binding pocket. Results In order to better understand this hairpin's role in DNA binding and helicase activity, we performed a detailed mutational and biochemical analysis of nine residues on this exterior β-hairpin (EXT-hp. We examined the activities of the mutants related to their helicase function, including hexamerization, ATPase, DNA binding and helicase activities. The assays showed that some of the residues on this EXT-hp play a role for DNA binding as well as for helicase activity. Conclusions These results implicate several current theories regarding helicase activity by this critical hexameric enzyme. As the data suggest that EXT-hp is involved in DNA binding, the results reported here imply that the EXT-hp located near the exterior exit of the side channels may play a role in contacting DNA substrate in a manner that affects DNA unwinding.

  4. Comparison of minichromosome maintenance proteins (MCM-3, MCM-7) and metallothioneins (MT-I/II, MT-III) expression in relation to clinicopathological data in ovarian cancer.

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    Kobierzycki, Christopher; Pula, Bartosz; Skiba, Mateusz; Jablonska, Karolina; Latkowski, Krzysztof; Zabel, Maciej; Nowak-Markwitz, Ewa; Spaczynski, Marek; Kedzia, Witold; Podhorska-Okolow, Marzena; Dziegiel, Piotr

    2013-12-01

    Despite great progress in the understanding of ovarian cancer biology, clinicopathological data (i.e. grade, stage, histological type and residual disease after surgery) seem to be the most important prognostic factors. The present study aimed to investigate the relationship between expression of minichromosome maintenance proteins (MCM-3, MCM-7), metallothioneins (MT-I/II, MT-III), and Ki-67 in 103 ovarian cancer cases, mostly of the serous histological type. Statistical analysis revealed strong positive correlations in the expression of MCM-3 vs. Ki-67 (r=0.492), MCM-7 vs. Ki-67 (r=0.651), and MCM-3 vs. MCM-7 (r=0.515) (all pMCM-3 and Ki-67 with increasing grade of histological malignancy (p=0.0011, p=0.029, respectively). Regarding clinical progression, cytoplasmic MT-I/II expression was significantly higher in more advanced disease stages (III+IV vs. I+II; p=0.0247). Due to the correlations shown here, the determination of MCM proteins as proliferation markers of ovarian cancer, should be strongly considered.

  5. The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.

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    Santosa, Venny; Martha, Sabrina; Hirose, Noriaki; Tanaka, Katsunori

    2013-03-08

    The minichromosome maintenance (MCM) complex is a replicative helicase, which is essential for chromosome DNA replication. In recent years, the identification of a novel MCM-binding protein (MCM-BP) in most eukaryotes has led to numerous studies investigating its function and its relationship to the MCM complex. However, the mechanisms by which MCM-BP functions and associates with MCM complexes are not well understood; in addition, the functional role of MCM-BP remains controversial and may vary between model organisms. The present study aims to elucidate the nature and biological function of the MCM-BP ortholog, Mcb1, in fission yeast. The Mcb1 protein continuously interacts with MCM proteins during the cell cycle in vivo and can interact with any individual MCM subunit in vitro. To understand the detailed characteristics of mcb1(+), two temperature-sensitive mcb1 gene mutants (mcb1(ts)) were isolated. Extensive genetic analysis showed that the mcb1(ts) mutants were suppressed by a mcm5(+) multicopy plasmid and displayed synthetic defects with many S-phase-related gene mutants. Moreover, cyclin-dependent kinase modulation by Cig2 repression or Rum1 overproduction suppressed the mcb1(ts) mutants, suggesting the involvement of Mcb1 in pre-RC formation during DNA replication. These data are consistent with the observation that Mcm7 loading onto replication origins is reduced and S-phase progression is delayed in mcb1(ts) mutants. Furthermore, the mcb1(ts) mutation led to the redistribution of MCM subunits to the cytoplasm, and this redistribution was dependent on an active nuclear export system. These results strongly suggest that Mcb1 promotes efficient pre-RC formation during DNA replication by regulating the MCM complex.

  6. The Fission Yeast Minichromosome Maintenance (MCM)-binding Protein (MCM-BP), Mcb1, Regulates MCM Function during Prereplicative Complex Formation in DNA Replication*

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    Santosa, Venny; Martha, Sabrina; Hirose, Noriaki; Tanaka, Katsunori

    2013-01-01

    The minichromosome maintenance (MCM) complex is a replicative helicase, which is essential for chromosome DNA replication. In recent years, the identification of a novel MCM-binding protein (MCM-BP) in most eukaryotes has led to numerous studies investigating its function and its relationship to the MCM complex. However, the mechanisms by which MCM-BP functions and associates with MCM complexes are not well understood; in addition, the functional role of MCM-BP remains controversial and may vary between model organisms. The present study aims to elucidate the nature and biological function of the MCM-BP ortholog, Mcb1, in fission yeast. The Mcb1 protein continuously interacts with MCM proteins during the cell cycle in vivo and can interact with any individual MCM subunit in vitro. To understand the detailed characteristics of mcb1+, two temperature-sensitive mcb1 gene mutants (mcb1ts) were isolated. Extensive genetic analysis showed that the mcb1ts mutants were suppressed by a mcm5+ multicopy plasmid and displayed synthetic defects with many S-phase-related gene mutants. Moreover, cyclin-dependent kinase modulation by Cig2 repression or Rum1 overproduction suppressed the mcb1ts mutants, suggesting the involvement of Mcb1 in pre-RC formation during DNA replication. These data are consistent with the observation that Mcm7 loading onto replication origins is reduced and S-phase progression is delayed in mcb1ts mutants. Furthermore, the mcb1ts mutation led to the redistribution of MCM subunits to the cytoplasm, and this redistribution was dependent on an active nuclear export system. These results strongly suggest that Mcb1 promotes efficient pre-RC formation during DNA replication by regulating the MCM complex. PMID:23322785

  7. Minichromosome Maintenance Expression Defines Slow-Growing Gastroenteropancreatic Neuroendocrine Neoplasms

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    Simon Schimmack

    2016-10-01

    Full Text Available BACKGROUND: Small intestinal neuroendocrine neoplasm (SI-NEN proliferation is quantified by Ki67 measurements which capture G1-G2M phases of the cell cycle. G0 and early G1 phases, typical of slow-growing cells, can be detected by minichromosome maintenance protein (MCM expression. We hypothesized that these replication licensing markers may provide clinically relevant information to augment Ki67 in low-grade neuroendocrine neoplasia. METHODS: Immunohistochemical staining (IHC, Western blot analysis, quantitative polymerase chain reaction, and copy number variations of MCM2, MCM3, and Ki67 were undertaken in SI-NENs (n = 22. MCM and Ki67 expression was compared by Kaplan-Meier survival analysis (tissue microarray, independent set [n = 55]. Forty-three pancreatic NENs and 14 normal tissues were included as controls. RESULTS: In SI-NENs, MCM2 (mean: 21.2%: range: 16%-25% and MCM3 (28.7%: 22%-34% were detected in significantly more cells than Ki67 (2.3%: 0%-7%, P < .01. MCM2 mRNA correlated with Ki67 IHC (P < .05. MCM3 protein expression was higher in metastases (38-fold than in normal small intestine (P = .06 and was largely absent in normal neuroendocrine cells. There was considerable variation at the MCM copy number level (0-4 copies. MCM3 expression in proliferating cells significantly predicted overall survival (P < .002. Combinations of Ki67 and MCM2/3 in algorithms differentiated low and higher proliferative lesions (overall survival: 12 vs 6.1 years, P = .06. MCM expression was not informative in pancreatic NENs. CONCLUSION: MCMs are expressed in a higher proportion of NEN cells than Ki67 in slow-growing small intestinal lesions and correlate with survival. Assessment can be used to augment Ki67 to improve prognostic classification in these low-grade tumors.

  8. Expression of minichromosome maintenance genes in renal cell carcinoma

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    Zhong HB

    2017-11-01

    Full Text Available Hongbin Zhong,1,* Bin Chen,1,* Henrique Neves,2 Jinchun Xing,1 Youxin Ye,1 Ying Lin,1 Guohong Zhuang,3 Shu-Dong Zhang,4 Jiyi Huang,1,5 Hang Fai Kwok2 1Xiang’an Branch, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, People’s Republic of China; 2Faculty of Health Sciences, University of Macau, Taipa, Macau SAR; 3Medical College of Xiamen University, Xiamen, Fujian, People’s Republic of China; 4Northern Ireland Centre for Stratified Medicine, Biomedical Sciences Research Institute, Ulster University, Londonderry, UK; 5The First Clinical School of Fujian Medical University, Fuzhou, Fujian, People’s Republic of China *These authors contributed equally to this work Abstract: Minichromosome maintenance (MCM proteins play an essential role in DNA replication. They have been shown to be overexpressed in various types of cancer. However, the role of this family in renal cell carcinoma (RCC is widely unknown. In this study, we have identified a number of RCC datasets in the Gene Expression Omnibus database and also investigated the correlation between the expression levels of MCM genes and clinicopathological parameters. We found that the expression levels of MCM genes are positively correlated with one another. Expression levels of MCM2, MCM5, MCM6, and MCM7, but not of MCM3 and MCM4, were higher in RCC compared to paired adjacent normal tissue. Only the expression level of MCM4, but not of other MCMs, was positively correlated with tumor grade. In addition, a high-level expression of MCM2 in either primary tumor or metastases of RCC predicted a shorter disease-free survival time, while a high-level expression of MCM4 or MCM6 in primary tumor was also associated with poorer disease-free survival. Interestingly, we also demonstrated that patients with their primary RCC overexpressing 2 or more MCM genes had a shorter disease-free survival time, while those with RCC metastases overexpressing 3 or more MCM genes had a shorter

  9. Phosphorylation of Minichromosome Maintenance 3 (MCM3) by Checkpoint Kinase 1 (Chk1) Negatively Regulates DNA Replication and Checkpoint Activation.

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    Han, Xiangzi; Mayca Pozo, Franklin; Wisotsky, Jacob N; Wang, Benlian; Jacobberger, James W; Zhang, Youwei

    2015-05-08

    Mechanisms controlling DNA replication and replication checkpoint are critical for the maintenance of genome stability and the prevention or treatment of human cancers. Checkpoint kinase 1 (Chk1) is a key effector protein kinase that regulates the DNA damage response and replication checkpoint. The heterohexameric minichromosome maintenance (MCM) complex is the core component of mammalian DNA helicase and has been implicated in replication checkpoint activation. Here we report that Chk1 phosphorylates the MCM3 subunit of the MCM complex at Ser-205 under normal growth conditions. Mutating the Ser-205 of MCM3 to Ala increased the length of DNA replication track and shortened the S phase duration, indicating that Ser-205 phosphorylation negatively controls normal DNA replication. Upon replicative stress treatment, the inhibitory phosphorylation of MCM3 at Ser-205 was reduced, and this reduction was accompanied with the generation of single strand DNA, the key platform for ataxia telangiectasia mutated and Rad3-related (ATR) activation. As a result, the replication checkpoint is activated. Together, these data provide significant insights into the regulation of both normal DNA replication and replication checkpoint activation through the novel phosphorylation of MCM3 by Chk1. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  10. Quantitative Proteomics Reveals Dynamic Interactions of the Minichromosome Maintenance Complex (MCM) in the Cellular Response to Etoposide Induced DNA Damage.

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    Drissi, Romain; Dubois, Marie-Line; Douziech, Mélanie; Boisvert, François-Michel

    2015-07-01

    The minichromosome maintenance complex (MCM) proteins are required for processive DNA replication and are a target of S-phase checkpoints. The eukaryotic MCM complex consists of six proteins (MCM2-7) that form a heterohexameric ring with DNA helicase activity, which is loaded on chromatin to form the pre-replication complex. Upon entry in S phase, the helicase is activated and opens the DNA duplex to recruit DNA polymerases at the replication fork. The MCM complex thus plays a crucial role during DNA replication, but recent work suggests that MCM proteins could also be involved in DNA repair. Here, we employed a combination of stable isotope labeling with amino acids in cell culture (SILAC)-based quantitative proteomics with immunoprecipitation of green fluorescent protein-tagged fusion proteins to identify proteins interacting with the MCM complex, and quantify changes in interactions in response to DNA damage. Interestingly, the MCM complex showed very dynamic changes in interaction with proteins such as Importin7, the histone chaperone ASF1, and the Chromodomain helicase DNA binding protein 3 (CHD3) following DNA damage. These changes in interactions were accompanied by an increase in phosphorylation and ubiquitination on specific sites on the MCM proteins and an increase in the co-localization of the MCM complex with γ-H2AX, confirming the recruitment of these proteins to sites of DNA damage. In summary, our data indicate that the MCM proteins is involved in chromatin remodeling in response to DNA damage. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  11. Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex*

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    Douglas, Max E.

    2016-01-01

    Mcm10 is required for the initiation of eukaryotic DNA replication and contributes in some unknown way to the activation of the Cdc45-MCM-GINS (CMG) helicase. How Mcm10 is localized to sites of replication initiation is unclear, as current models indicate that direct binding to minichromosome maintenance (MCM) plays a role, but the details and functional importance of this interaction have not been determined. Here, we show that purified Mcm10 can bind both DNA-bound double hexamers and soluble single hexamers of MCM. The binding of Mcm10 to MCM requires the Mcm10 C terminus. Moreover, the binding site for Mcm10 on MCM includes the Mcm2 and Mcm6 subunits and overlaps that for the loading factor Cdt1. Whether Mcm10 recruitment to replication origins depends on CMG helicase assembly has been unclear. We show that Mcm10 recruitment occurs via two modes: low affinity recruitment in the absence of CMG assembly (“G1-like”) and high affinity recruitment when CMG assembly takes place (“S-phase-like”). Mcm10 that cannot bind directly to MCM is defective in both modes of recruitment and is unable to support DNA replication. These findings indicate that Mcm10 is localized to replication initiation sites by directly binding MCM through the Mcm10 C terminus. PMID:26719337

  12. Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.

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    Douglas, Max E; Diffley, John F X

    2016-03-11

    Mcm10 is required for the initiation of eukaryotic DNA replication and contributes in some unknown way to the activation of the Cdc45-MCM-GINS (CMG) helicase. How Mcm10 is localized to sites of replication initiation is unclear, as current models indicate that direct binding to minichromosome maintenance (MCM) plays a role, but the details and functional importance of this interaction have not been determined. Here, we show that purified Mcm10 can bind both DNA-bound double hexamers and soluble single hexamers of MCM. The binding of Mcm10 to MCM requires the Mcm10 C terminus. Moreover, the binding site for Mcm10 on MCM includes the Mcm2 and Mcm6 subunits and overlaps that for the loading factor Cdt1. Whether Mcm10 recruitment to replication origins depends on CMG helicase assembly has been unclear. We show that Mcm10 recruitment occurs via two modes: low affinity recruitment in the absence of CMG assembly ("G1-like") and high affinity recruitment when CMG assembly takes place ("S-phase-like"). Mcm10 that cannot bind directly to MCM is defective in both modes of recruitment and is unable to support DNA replication. These findings indicate that Mcm10 is localized to replication initiation sites by directly binding MCM through the Mcm10 C terminus. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  13. The Mini-Chromosome Maintenance (Mcm) Complexes Interact with DNA Polymerase α-Primase and Stimulate Its Ability to Synthesize RNA Primers

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    You, Zhiying; De Falco, Mariarosaria; Kamada, Katsuhiko; Pisani, Francesca M.; Masai, Hisao

    2013-01-01

    The Mini-chromosome maintenance (Mcm) proteins are essential as central components for the DNA unwinding machinery during eukaryotic DNA replication. DNA primase activity is required at the DNA replication fork to synthesize short RNA primers for DNA chain elongation on the lagging strand. Although direct physical and functional interactions between helicase and primase have been known in many prokaryotic and viral systems, potential interactions between helicase and primase have not been explored in eukaryotes. Using purified Mcm and DNA primase complexes, a direct physical interaction is detected in pull-down assays between the Mcm2∼7 complex and the hetero-dimeric DNA primase composed of the p48 and p58 subunits. The Mcm4/6/7 complex co-sediments with the primase and the DNA polymerase α-primase complex in glycerol gradient centrifugation and forms a Mcm4/6/7-primase-DNA ternary complex in gel-shift assays. Both the Mcm4/6/7 and Mcm2∼7 complexes stimulate RNA primer synthesis by DNA primase in vitro. However, primase inhibits the Mcm4/6/7 helicase activity and this inhibition is abolished by the addition of competitor DNA. In contrast, the ATP hydrolysis activity of Mcm4/6/7 complex is not affected by primase. Mcm and primase proteins mutually stimulate their DNA-binding activities. Our findings indicate that a direct physical interaction between primase and Mcm proteins may facilitate priming reaction by the former protein, suggesting that efficient DNA synthesis through helicase-primase interactions may be conserved in eukaryotic chromosomes. PMID:23977294

  14. The mini-chromosome maintenance (Mcm complexes interact with DNA polymerase α-primase and stimulate its ability to synthesize RNA primers.

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    Zhiying You

    Full Text Available The Mini-chromosome maintenance (Mcm proteins are essential as central components for the DNA unwinding machinery during eukaryotic DNA replication. DNA primase activity is required at the DNA replication fork to synthesize short RNA primers for DNA chain elongation on the lagging strand. Although direct physical and functional interactions between helicase and primase have been known in many prokaryotic and viral systems, potential interactions between helicase and primase have not been explored in eukaryotes. Using purified Mcm and DNA primase complexes, a direct physical interaction is detected in pull-down assays between the Mcm2~7 complex and the hetero-dimeric DNA primase composed of the p48 and p58 subunits. The Mcm4/6/7 complex co-sediments with the primase and the DNA polymerase α-primase complex in glycerol gradient centrifugation and forms a Mcm4/6/7-primase-DNA ternary complex in gel-shift assays. Both the Mcm4/6/7 and Mcm2~7 complexes stimulate RNA primer synthesis by DNA primase in vitro. However, primase inhibits the Mcm4/6/7 helicase activity and this inhibition is abolished by the addition of competitor DNA. In contrast, the ATP hydrolysis activity of Mcm4/6/7 complex is not affected by primase. Mcm and primase proteins mutually stimulate their DNA-binding activities. Our findings indicate that a direct physical interaction between primase and Mcm proteins may facilitate priming reaction by the former protein, suggesting that efficient DNA synthesis through helicase-primase interactions may be conserved in eukaryotic chromosomes.

  15. The Interaction between Checkpoint Kinase 1 (Chk1) and the Minichromosome Maintenance (MCM) Complex Is Required for DNA Damage-induced Chk1 Phosphorylation*

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    Han, Xiangzi; Aslanian, Aaron; Fu, Kang; Tsuji, Toshiya; Zhang, Youwei

    2014-01-01

    Chk1 is an essential mediator of the DNA damage response and cell cycle checkpoint. However, how exactly Chk1 transduces the checkpoint signaling is not fully understood. Here we report the identification of the heterohexamic minichromosome maintenance (MCM) complex that interacts with Chk1 by mass spectrometry. The interaction between Chk1 and the MCM complex was reduced by DNA damage treatment. We show that the MCM complex, at least partially, contributes to the chromatin association of Chk1, allowing for immediate phosphorylation of Chk1 by ataxia telangiectasia mutated and Rad3-related (ATR) in the presence of DNA damage. Further, phosphorylation of Chk1 at ATR sites reduces the interaction between Chk1 and the MCM complex, facilitating chromatin release of phosphorylated Chk1, a critical step in the initiation and amplification of cell cycle checkpoint. Together, these data provide novel insights into the activation of Chk1 in response to DNA damage. PMID:25049228

  16. Minichromosomes: Vectors for Crop Improvement

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    Jon P. Cody; Nathan C. Swyers; Morgan E. McCaw; Nathaniel D. Graham; Changzeng Zhao; James A. Birchler

    2015-01-01

    Minichromosome technology has the potential to offer a number of possibilities for expanding current biofortification strategies. While conventional genome manipulations rely on random integration of one or a few genes, engineered minichromosomes would enable researchers to concatenate several gene aggregates into a single independent chromosome. These engineered minichromosomes can be rapidly transferred as a unit to other lines through the utilization of doubled haploid breeding. If used ...

  17. Minichromosomes: Vectors for Crop Improvement

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    Jon P. Cody

    2015-07-01

    Full Text Available Minichromosome technology has the potential to offer a number of possibilities for expanding current biofortification strategies. While conventional genome manipulations rely on random integration of one or a few genes, engineered minichromosomes would enable researchers to concatenate several gene aggregates into a single independent chromosome. These engineered minichromosomes can be rapidly transferred as a unit to other lines through the utilization of doubled haploid breeding. If used in conjunction with other biofortification methods, it may be possible to significantly increase the nutritional value of crops.

  18. Minichromosome maintenance helicase paralog MCM9 is dispensible for DNA replication but functions in germ-line stem cells and tumor suppression.

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    Hartford, Suzanne A; Luo, Yunhai; Southard, Teresa L; Min, Irene M; Lis, John T; Schimenti, John C

    2011-10-25

    Effective DNA replication is critical to the health and reproductive success of organisms. The six MCM2-7 proteins, which form the replicative helicase, are essential for high-fidelity replication of the genome. Many eukaryotes have a divergent paralog, MCM9, that was reported to be essential for loading MCM2-7 onto replication origins in the Xenopus oocyte extract system. To address the in vivo role of mammalian MCM9, we created and analyzed the phenotypes of mice with various mutations in Mcm9 and an intronic DNA replication-related gene Asf1a. Ablation of Mcm9 was compatible with cell proliferation and mouse viability, showing that it is nonessential for MCM2-7 loading or DNA replication. Mcm9 mutants underwent p53-independent embryonic germ-cell depletion in both sexes, with males also exhibiting defective spermatogonial stem-cell renewal. MCM9-deficient cells had elevated genomic instability and defective cell cycle reentry following replication stress, and mutant animals were prone to sex-specific cancers, most notably hepatocellular carcinoma in males. The phenotypes of mutant mice and cells suggest that MCM9 evolved a specialized but nonessential role in DNA replication or replication-linked quality-control mechanisms that are especially important for germ-line stem cells, and also for tumor suppression and genome maintenance in the soma.

  19. Retinoid X Receptor α-Dependent HBV Minichromosome Remodeling and Viral Replication.

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    Zhang, Yan; He, Song; Guo, Jin-Jun; Peng, Hong; Fan, Jia-Hao; Li, Qing-Ling

    2017-01-01

    The HBV covalently closed circular DNA (cccDNA) is organized into a minichromosome in the nuclei of infected hepatocytes through interactions with histone and nonhistone proteins. Retinoid X receptor α (RXRα), a liver-enriched nuclear receptor, participates in regulation of HBV replication and transcription through modulation of HBV enhancer 1 and core promoter activity. This study investigated RXRα involvement in HBV cccDNA epigenetic modifications. Quantitative cccDNA chromatin immunoprecipitation (ChIP) was applied to study the recruitment of RXRα, histones, and chromatin-modifying enzymes to HBV minichromosome in HepG2 cells after transfection of the linear HBV genome. RXRα Was found to directly bind to HBV cccDNA; recruitment of RXRα to HBV mini-chromosome paralleled HBV replication, histone recruitment, and histone acetylation in HBVcccDNA. Moreover, RXRα overexpression or knock-down significantly increased or impaired the recruitment of the p300 acetyltransferase to cccDNAminichromosome. Our results confirmed the regulation of RXRα on HBV replication in vitro and demonstrated the modulation of RXRα on HBV cccDNA epigenetics. These findings provide a profound theoretical and experimental basis for late-model antiviral treatment acting on the HBV cccDNA and minichromosome.

  20. Fanconi anemia proteins in telomere maintenance.

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    Sarkar, Jaya; Liu, Yie

    2016-07-01

    Mammalian chromosome ends are protected by nucleoprotein structures called telomeres. Telomeres ensure genome stability by preventing chromosome termini from being recognized as DNA damage. Telomere length homeostasis is inevitable for telomere maintenance because critical shortening or over-lengthening of telomeres may lead to DNA damage response or delay in DNA replication, and hence genome instability. Due to their repetitive DNA sequence, unique architecture, bound shelterin proteins, and high propensity to form alternate/secondary DNA structures, telomeres are like common fragile sites and pose an inherent challenge to the progression of DNA replication, repair, and recombination apparatus. It is conceivable that longer the telomeres are, greater is the severity of such challenges. Recent studies have linked excessively long telomeres with increased tumorigenesis. Here we discuss telomere abnormalities in a rare recessive chromosomal instability disorder called Fanconi Anemia and the role of the Fanconi Anemia pathway in telomere biology. Reports suggest that Fanconi Anemia proteins play a role in maintaining long telomeres, including processing telomeric joint molecule intermediates. We speculate that ablation of the Fanconi Anemia pathway would lead to inadequate aberrant structural barrier resolution at excessively long telomeres, thereby causing replicative burden on the cell. Published by Elsevier B.V.

  1. The Argonaute protein TbAGO1 contributes to large and mini-chromosome segregation and is required for control of RIME retroposons and RHS pseudogene-associated transcripts.

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    Durand-Dubief, Mickaël; Absalon, Sabrina; Menzer, Linda; Ngwabyt, Sandra; Ersfeld, Klaus; Bastin, Philippe

    2007-12-01

    The protist Trypanosoma brucei possesses a single Argonaute gene called TbAGO1 that is necessary for RNAi silencing. We previously showed that in strain 427, TbAGO1 knock-out leads to a slow growth phenotype and to chromosome segregation defects. Here we report that the slow growth phenotype is linked to defects in segregation of both large and mini-chromosome populations, with large chromosomes being the most affected. These phenotypes are completely reversed upon inducible re-expression of TbAGO1 fused to GFP, demonstrating their link with TbAGO1. Trypanosomes that do not express TbAGO1 show a general increase in the abundance of transcripts derived from the short retroposon RIME (Ribosomal Interspersed Mobile Element). Supplementary large RIME transcripts emerge in the absence of RNAi, a phenomenon coupled to the disappearance of short transcripts. These fluctuations are reversed by inducible expression of GFP::TbAGO1. Furthermore, we use a combination of Northern blots, RT-PCR and sequencing to reveal that RNAi controls expression of transcripts derived from RHS (Retrotransposon Hot Spot) pseudogenes (RHS genes with retro-element(s) integrated within their coding sequence). Absence of RNAi also leads to an increase of steady-state transcripts from regular RHS genes (those without retro-element), indicating a role for pseudogene in control of gene expression. However, analysis of retroposon abundance and arrangement in the genome of multiple clonal cell lines of TbAGO1-/- failed to reveal movement of mobile elements despite the increased amounts of retroposon transcripts.

  2. Naturally occurring minichromosome platforms in chromosome engineering: an overview.

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    Raimondi, Elena

    2011-01-01

    Artificially modified chromosome vectors are non-integrating gene delivery platforms that can shuttle very large DNA fragments in various recipient cells: theoretically, no size limit exists for the chromosome segments that an engineered minichromosome can accommodate. Therefore, genetically manipulated chromosomes might be potentially ideal vector systems, especially when the complexity of higher eukaryotic genes is concerned. This review focuses on those chromosome vectors generated using spontaneously occurring small markers as starting material. The definition and manipulation of the centromere domain is one of the main obstacles in chromosome engineering: naturally occurring minichromosomes, due to their inherent small size, were helpful in defining some aspects of centromere function. In addition, several distinctive features of small marker chromosomes, like their appearance as supernumerary elements in otherwise normal karyotypes, have been successfully exploited to use them as gene delivery vectors. The key technologies employed for minichromosome engineering are: size reduction, gene targeting, and vector delivery in various recipient cells. In spite of the significant advances that have been recently achieved in all these fields, several unsolved problems limit the potential of artificially modified chromosomes. Still, these vector systems have been exploited in a number of applications where the investigation of the controlled expression of large DNA segments is needed. A typical example is the analysis of genes whose expression strictly depends on the chromosomal environment in which they are positioned, where engineered chromosomes can be envisaged as epigenetically regulated expression systems. A novel and exciting advance concerns the use of engineered minichromosomes to study the organization and dynamics of local chromatin structures.

  3. Protein interactions in genome maintenance as novel antibacterial targets.

    Directory of Open Access Journals (Sweden)

    Aimee H Marceau

    Full Text Available Antibacterial compounds typically act by directly inhibiting essential bacterial enzyme activities. Although this general mechanism of action has fueled traditional antibiotic discovery efforts for decades, new antibiotic development has not kept pace with the emergence of drug resistant bacterial strains. These limitations have severely restricted the therapeutic tools available for treating bacterial infections. Here we test an alternative antibacterial lead-compound identification strategy in which essential protein-protein interactions are targeted rather than enzymatic activities. Bacterial single-stranded DNA-binding proteins (SSBs form conserved protein interaction "hubs" that are essential for recruiting many DNA replication, recombination, and repair proteins to SSB/DNA nucleoprotein substrates. Three small molecules that block SSB/protein interactions are shown to have antibacterial activity against diverse bacterial species. Consistent with a model in which the compounds target multiple SSB/protein interactions, treatment of Bacillus subtilis cultures with the compounds leads to rapid inhibition of DNA replication and recombination, and ultimately to cell death. The compounds also have unanticipated effects on protein synthesis that could be due to a previously unknown role for SSB/protein interactions in translation or to off-target effects. Our results highlight the potential of targeting protein-protein interactions, particularly those that mediate genome maintenance, as a powerful approach for identifying new antibacterial compounds.

  4. Optimum dietary protein requirement in nondiabetic maintenance hemodialysis patients.

    Science.gov (United States)

    Ohkawa, Sakae; Kaizu, Yukiko; Odamaki, Mari; Ikegaya, Naoki; Hibi, Ikuo; Miyaji, Kunihiko; Kumagai, Hiromichi

    2004-03-01

    There is controversy about whether the dietary protein requirement of 1.2 g/kg/d for hemodialysis (HD) patients, in the nutritional guidelines recommended by the National Kidney Foundation-Kidney Disease Outcomes Quality Initiative (NKF-KDOQI), is reasonable. A cross-sectional study was conducted in 129 stable HD patients without diabetes (84 men, 45 women) to investigate the association between the protein equivalent of nitrogen appearance normalized by ideal body weight (nPNAibw), an index of protein intake, and skeletal muscle mass or other metabolic consequences. Patients were divided into 5 groups according to nPNAibw index. Midthigh muscle area (TMA), midthigh subcutaneous fat area (TSFA), abdominal muscle area (AMA), abdominal subcutaneous fat area (ASFA), and visceral fat area (AVFA) were measured using computed tomography, and various nutritional parameters were compared among these groups. TMA and AMA values increased with increasing dietary protein intake from less than 0.7 g/kg/d to 0.9-1.1 g/kg/d and showed a plateau at greater than 0.9 to 1.1 g/kg/d of dietary protein intake. Conversely, fat mass, including TSFA, ASFA, and AVFA, and serum potassium concentration increased with graded protein intake, and no plateau was formed. Patients with nPNAibw greater than 1.3 g/kg/d satisfied the criterion of visceral obesity. Although serum prealbumin levels showed a trend similar to that of muscle mass, there was no significant difference in serum albumin levels among the study groups. Optimal dietary protein requirement for patients undergoing maintenance HD in a stable condition appears to be less than the level recommended by the NKF-KDOQI nutritional guidelines.

  5. Variation in mitochondrial minichromosome composition between blood-sucking lice of the genus Haematopinus that infest horses and pigs.

    Science.gov (United States)

    Song, Simon D; Barker, Stephen C; Shao, Renfu

    2014-03-31

    The genus Haematopinus contains 21 species of blood-sucking lice, parasitizing both even-toed ungulates (pigs, cattle, buffalo, antelopes, camels and deer) and odd-toed ungulates (horses, donkeys and zebras). The mitochondrial genomes of the domestic pig louse, Haematopinus suis, and the wild pig louse, Haematopinus apri, have been sequenced recently; both lice have fragmented mitochondrial genomes with 37 genes on nine minichromosomes. To understand whether the composition of mitochondrial minichromosomes and the gene content and gene arrangement of each minichromosome are stable within the genus, we sequenced the mitochondrial genome of the horse louse, Haematopinus asini. We used a PCR-based strategy to amplify four mitochondrial minichromosomes in near full-length, and then amplify the entire coding regions of all of the nine mitochondrial minichromosomes of the horse louse. These amplicons were sequenced with an Illumina Hiseq platform. We identified all of the 37 mitochondrial genes typical of bilateral animals in the horse louse, Haematopinus asini; these genes are on nine circular minichromosomes. Each minichromosome is 3.5-5.0 kb in size and consists of a coding region and a non-coding region except R-nad4L-rrnS-C minichromosome, which contains two coding regions and two non-coding regions. Six of the nine minichromosomes of the horse louse have their counterparts in the pig lice with the same gene content and gene arrangement. However, the gene content and arrangement of the other three minichromosomes of the horse louse, including R-nad4L-rrnS-C, are different from that of the other three minichromosomes of the pig lice. Comparison between the horse louse and the pig lice revealed variation in the composition of mitochondrial minichromosomes within the genus Haematopinus, which can be accounted for by gene translocation events between minichromosomes. The current study indicates that inter-minichromosome recombination plays a major role in generating the

  6. The role of protein in weight loss and maintenance

    DEFF Research Database (Denmark)

    Leidy, Heather J; Clifton, Peter M; Astrup, Arne

    2015-01-01

    Over the past 20 y, higher-protein diets have been touted as a successful strategy to prevent or treat obesity through improvements in body weight management. These improvements are thought to be due, in part, to modulations in energy metabolism, appetite, and energy intake. Recent evidence also...... to be the primary contributor to the discrepant findings because improvements in weight management were detected in those who adhered to the prescribed higher-protein regimen, whereas those who did not adhere to the diet had no marked improvements. Collectively, these data suggest that higher-protein diets...... that contain between 1.2 and 1.6 g protein · kg(-1) · d(-1) and potentially include meal-specific protein quantities of at least ∼25-30 g protein/meal provide improvements in appetite, body weight management, cardiometabolic risk factors, or all of these health outcomes; however, further strategies to increase...

  7. Blood profiling of proteins and steroids during weight maintenance with manipulation of dietary protein level and glycaemic index

    DEFF Research Database (Denmark)

    Wang, Ping; Holst, Claus; Astrup, Arne

    2012-01-01

    ) blood biomarkers of dietary protein and GI levels during the weight-maintenance phase. Blood samples were collected at baseline, after 8 weeks of low-energy diet-induced weight loss and after a 6-month dietary intervention period from female continued weight losers (n 48) and weight regainers (n 48......), evenly selected from four dietary groups that varied in protein and GI levels. The blood concentrations of twenty-nine proteins and three steroid hormones were measured. The changes in analytes during weight maintenance largely correlated negatively with the changes during weight loss, with some...

  8. The role of protein in weight loss and maintenance.

    Science.gov (United States)

    Leidy, Heather J; Clifton, Peter M; Astrup, Arne; Wycherley, Thomas P; Westerterp-Plantenga, Margriet S; Luscombe-Marsh, Natalie D; Woods, Stephen C; Mattes, Richard D

    2015-04-29

    Over the past 20 y, higher-protein diets have been touted as a successful strategy to prevent or treat obesity through improvements in body weight management. These improvements are thought to be due, in part, to modulations in energy metabolism, appetite, and energy intake. Recent evidence also supports higher-protein diets for improvements in cardiometabolic risk factors. This article provides an overview of the literature that explores the mechanisms of action after acute protein consumption and the clinical health outcomes after consumption of long-term, higher-protein diets. Several meta-analyses of shorter-term, tightly controlled feeding studies showed greater weight loss, fat mass loss, and preservation of lean mass after higher-protein energy-restriction diets than after lower-protein energy-restriction diets. Reductions in triglycerides, blood pressure, and waist circumference were also reported. In addition, a review of the acute feeding trials confirms a modest satiety effect, including greater perceived fullness and elevated satiety hormones after higher-protein meals but does not support an effect on energy intake at the next eating occasion. Although shorter-term, tightly controlled feeding studies consistently identified benefits with increased protein consumption, longer-term studies produced limited and conflicting findings; nevertheless, a recent meta-analysis showed persistent benefits of a higher-protein weight-loss diet on body weight and fat mass. Dietary compliance appears to be the primary contributor to the discrepant findings because improvements in weight management were detected in those who adhered to the prescribed higher-protein regimen, whereas those who did not adhere to the diet had no marked improvements. Collectively, these data suggest that higher-protein diets that contain between 1.2 and 1.6 g protein · kg -1 · d -1 and potentially include meal-specific protein quantities of at least ∼25-30 g protein/meal provide

  9. New method for the quality check of food proteins of the maintenance metabolism. 4

    International Nuclear Information System (INIS)

    Simon, O.; Hernandez, M.; Bergner, H.

    1981-01-01

    Male adult rats (370 g body weight) were fed on maintenance level (460 kJ ME/kgsup(0,75). In a 10 days preliminary period they received a casein/methionine (95/5) diet supplemented with 10 mg 15 N excess per 0.178 kg metabolic body weight in form of ammonium acetate. Thereafter the animals were put on 8 isonitrogenous diets containing as protein sources casein, soya protein, gelatine, whole-egg, fish meal, pea, wheat and yeast. The 15 N excretion via urine and feces was used to evaluate the dietary proteins for maintenance. 15 N in urine was lowest in animals fed on wheat diet and highest after feeding whole-egg diet. From these data a so called ' 15 N excretion biological valence (BV)' was calculated, which indicated the highest quality for wheat and soy protein in meeting the needs of the intermediary maintenance metabolism. On the other hand, dietary protein sources influence the loss of endogenous nitrogen as metabolic fecal nitrogen (MFN). It was found to be lowest in animals fed on diets containing isolated proteins (6 mg MFN/100 g body weight) and highest after feeding protein sources of plant origin with a high content in crude fibre (10 mg MFN/100 g). Both, losses of 15 N via urine and via feces were combined in a parameter called 'total BV'. According to this parameter the differences in quality for maintenance were only little between the protein sources tested (casein 100, soy protein 100, pea 99, wheat 99, whole egg 92, fish meal 89, gelatin 89). It was concluded that in the state of maintenance the supply with essential amino acids is not critical and that the supply with dispensable amino acids (or nonspecific nitrogen) is of great importance. (author)

  10. Cryo-electron microscopy of vitrified SV40 minichromosomes: the liquid drop model.

    Science.gov (United States)

    Dubochet, J; Adrian, M; Schultz, P; Oudet, P

    1986-03-01

    The structure of SV40 minichromosomes has been studied by cryo-electron microscopy of vitrified thin layers of solution. In high-salt buffer (130 mM NaCl), freshly prepared minichromosomes are condensed into globules 30 nm or more in diameter. On the micrograph, they appear to be formed by the close packing of 10 nm granules which give rise to a 10 nm reflection in the optical diffractogram. The globules can adopt many different conformations. At high concentration, they fuse into a homogeneous 'sea' of closely packed 10 nm granules. In low-salt buffer (less than 10 mM NaCl), the globules open, first into 10 nm filaments, and then into nucleosome-strings. The 'liquid drop' model is proposed to explain the condensed structure of the minichromosome in high-salt buffer: nucleosomes stack specifically on top of one another, thus forming the 10 nm filaments. 10 nm filaments in turn, tend to aggregate laterally. Optimizing both these interactions results in the condensation of 10 nm filaments or portions thereof into a structure similar to that of a liquid. Some implications of this model for the structure of cellular chromatin are discussed.

  11. Protein kinase M ζ and the maintenance of long-term memory.

    Science.gov (United States)

    Zhang, Yang; Zong, Wei; Zhang, Lei; Ma, Yuanye; Wang, Jianhong

    2016-10-01

    Although various molecules have been found to mediate the processes of memory acquisition and consolidation, the molecular mechanism to maintain memory still remains elusive. In recent years, a molecular pathway focusing on protein kinase Mζ (PKMζ) has become of interest to researchers because of its potential role in long-term memory maintenance. PKMζ is an isoform of protein kinase C (PKC) and has a related structure that influences its function in maintaining memory. Considerable evidence has been gathered on PKMζ activity, including loss of function studies using PKMζ inhibitors, such as PKMζ inhibitory peptide (ZIP), suggesting PKMζ plays an important role in long-term memory maintenance. This review provides an overview of the role of PKMζ in long-term memory and outlines the molecular structure of PKMζ, the molecular mechanism of PKMζ in long-term memory maintenance and future directions of PKMζ research. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Impact of weight loss and maintenance with ad libitum diets varying in protein and glycemic index content on metabolic syndrome

    DEFF Research Database (Denmark)

    Papadaki, Angeliki; Linardakis, Manolis; Plada, Maria

    2014-01-01

    We investigated the effects of weight loss and maintenance with diets that varied with regard to protein content and glycemic index (GI) on metabolic syndrome (MetSyn) status.......We investigated the effects of weight loss and maintenance with diets that varied with regard to protein content and glycemic index (GI) on metabolic syndrome (MetSyn) status....

  13. Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae.

    Science.gov (United States)

    Hoang, Margaret L; Leon, Ronald P; Pessoa-Brandao, Luis; Hunt, Sonia; Raghuraman, M K; Fangman, Walton L; Brewer, Bonita J; Sclafani, Robert A

    2007-11-01

    Eukaryotic chromosomal replication is a complicated process with many origins firing at different efficiencies and times during S phase. Prereplication complexes are assembled on all origins in G(1) phase, and yet only a subset of complexes is activated during S phase by DDK (for Dbf4-dependent kinase) (Cdc7-Dbf4). The yeast mcm5-bob1 (P83L) mutation bypasses DDK but results in reduced intrinsic firing efficiency at 11 endogenous origins and at origins located on minichromosomes. Origin efficiency may result from Mcm5 protein assuming an altered conformation, as predicted from the atomic structure of an archaeal MCM (for minichromosome maintenance) homologue. Similarly, an intragenic mutation in a residue predicted to interact with P83L suppresses the mcm5-bob1 bypass phenotype. We propose DDK phosphorylation of the MCM complex normally results in a single, highly active conformation of Mcm5, whereas the mcm5-bob1 mutation produces a number of conformations, only one of which is permissive for origin activation. Random adoption of these alternate states by the mcm5-bob1 protein can explain both how origin firing occurs independently of DDK and why origin efficiency is reduced. Because similar mutations in mcm2 and mcm4 cannot bypass DDK, Mcm5 protein may be a unique Mcm protein that is the final target of DDK regulation.

  14. Regulated eukaryotic DNA replication origin firing with purified proteins.

    Science.gov (United States)

    Yeeles, Joseph T P; Deegan, Tom D; Janska, Agnieszka; Early, Anne; Diffley, John F X

    2015-03-26

    Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated to promote precise genome duplication in every cell cycle. To accomplish this, initiation is partitioned into two temporally discrete steps: a double hexameric minichromosome maintenance (MCM) complex is first loaded at replication origins during G1 phase, and then converted to the active CMG (Cdc45-MCM-GINS) helicase during S phase. Here we describe the reconstitution of budding yeast DNA replication initiation with 16 purified replication factors, made from 42 polypeptides. Origin-dependent initiation recapitulates regulation seen in vivo. Cyclin-dependent kinase (CDK) inhibits MCM loading by phosphorylating the origin recognition complex (ORC) and promotes CMG formation by phosphorylating Sld2 and Sld3. Dbf4-dependent kinase (DDK) promotes replication by phosphorylating MCM, and can act either before or after CDK. These experiments define the minimum complement of proteins, protein kinase substrates and co-factors required for regulated eukaryotic DNA replication.

  15. V(D)J recombination on minichromosomes is not affected by transcription.

    Science.gov (United States)

    Hsieh, C L; McCloskey, R P; Lieber, M R

    1992-08-05

    It has been shown previously by others that transcription is temporally correlated with the onset of V(D)J recombination at the endogenous antigen receptor loci. We have been interested in determining whether this temporal correlation indicates a causal connection between these two processes. We have compared V(D)J recombination minichromosome substrates that have transcripts running through the recombination zone with substrates that do not in a transient transfection assay. In this system, the substrates acquire a minichromosome conformation within the first several hours after transfection. We find that the substrates recombine equally well over a 100-fold range in transcriptional variation. In additional studies, we have taken substrates that have low levels of transcription and inhibited transcription further by methylating the substrate DNA or by treating the cells with a general transcription inhibitor (alpha-amanitin). Although these treatments decrease the level of expression an additional 10-100-fold, there is still no observable effect on V(D)J recombination. Based on these results, we conclude that transcription is not necessary for the V(D)J reaction mechanism and does not alter substrate structure at the DNA level or at the simplest levels of chromatin structure in a way that affects the reaction.

  16. Proteomic analysis of the dysferlin protein complex unveils its importance for sarcolemmal maintenance and integrity.

    Directory of Open Access Journals (Sweden)

    Antoine de Morrée

    Full Text Available Dysferlin is critical for repair of muscle membranes after damage. Mutations in dysferlin lead to a progressive muscular dystrophy. Recent studies suggest additional roles for dysferlin. We set out to study dysferlin's protein-protein interactions to obtain comprehensive knowledge of dysferlin functionalities in a myogenic context. We developed a robust and reproducible method to isolate dysferlin protein complexes from cells and tissue. We analyzed the composition of these complexes in cultured myoblasts, myotubes and skeletal muscle tissue by mass spectrometry and subsequently inferred potential protein functions through bioinformatics analyses. Our data confirm previously reported interactions and support a function for dysferlin as a vesicle trafficking protein. In addition novel potential functionalities were uncovered, including phagocytosis and focal adhesion. Our data reveal that the dysferlin protein complex has a dynamic composition as a function of myogenic differentiation. We provide additional experimental evidence and show dysferlin localization to, and interaction with the focal adhesion protein vinculin at the sarcolemma. Finally, our studies reveal evidence for cross-talk between dysferlin and its protein family member myoferlin. Together our analyses show that dysferlin is not only a membrane repair protein but also important for muscle membrane maintenance and integrity.

  17. Non-essential MCM-related proteins mediate a response to DNA damage in the archaeon Methanococcus maripaludis.

    Science.gov (United States)

    Walters, Alison D; Chong, James P J

    2017-05-01

    The single minichromosome maintenance (MCM) protein found in most archaea has been widely studied as a simplified model for the MCM complex that forms the catalytic core of the eukaryotic replicative helicase. Organisms of the order Methanococcales are unusual in possessing multiple MCM homologues. The Methanococcus maripaludis S2 genome encodes four MCM homologues, McmA-McmD. DNA helicase assays reveal that the unwinding activity of the three MCM-like proteins is highly variable despite sequence similarities and suggests additional motifs that influence MCM function are yet to be identified. While the gene encoding McmA could not be deleted, strains harbouring individual deletions of genes encoding each of the other MCMs display phenotypes consistent with these proteins modulating DNA damage responses. M. maripaludis S2 is the first archaeon in which MCM proteins have been shown to influence the DNA damage response.

  18. Membrane Stored Curvature Elastic Stress Modulates Recruitment of Maintenance Proteins PspA and Vipp1.

    Science.gov (United States)

    McDonald, Christopher; Jovanovic, Goran; Ces, Oscar; Buck, Martin

    2015-09-01

    Phage shock protein A (PspA), which is responsible for maintaining inner membrane integrity under stress in enterobacteria, and vesicle-inducting protein in plastids 1 (Vipp1), which functions for membrane maintenance and thylakoid biogenesis in cyanobacteria and plants, are similar peripheral membrane-binding proteins. Their homologous N-terminal amphipathic helices are required for membrane binding; however, the membrane features recognized and required for expressing their functionalities have remained largely uncharacterized. Rigorously controlled, in vitro methodologies with lipid vesicles and purified proteins were used in this study and provided the first biochemical and biophysical characterizations of membrane binding by PspA and Vipp1. Both proteins are found to sense stored curvature elastic (SCE) stress and anionic lipids within the membrane. PspA has an enhanced sensitivity for SCE stress and a higher affinity for the membrane than Vipp1. These variations in binding may be crucial for some of the proteins' differing roles in vivo. Assays probing the transcriptional regulatory function of PspA in the presence of vesicles showed that a relief of transcription inhibition occurs in an SCE stress-specific manner. This in vitro recapitulation of membrane stress-dependent transcription control suggests that the Psp response may be mounted in vivo when a cell's inner membrane experiences increased SCE stress. All cell types maintain the integrity of their membrane systems. One widely distributed membrane stress response system in bacteria is the phage shock protein (Psp) system. The central component, peripheral membrane protein PspA, which mitigates inner membrane stress in bacteria, has a counterpart, Vipp1, which functions for membrane maintenance and thylakoid biogenesis in plants and photosynthetic bacteria. Membrane association of both these proteins is accepted as playing a pivotal role in their functions. Here we show that direct membrane binding by

  19. An All-Recombinant Protein-Based Culture System Specifically Identifies Hematopoietic Stem Cell Maintenance Factors

    Directory of Open Access Journals (Sweden)

    Aki Ieyasu

    2017-03-01

    Full Text Available Hematopoietic stem cells (HSCs are considered one of the most promising therapeutic targets for the treatment of various blood disorders. However, due to difficulties in establishing stable maintenance and expansion of HSCs in vitro, their insufficient supply is a major constraint to transplantation studies. To solve these problems we have developed a fully defined, all-recombinant protein-based culture system. Through this system, we have identified hemopexin (HPX and interleukin-1α as responsible for HSC maintenance in vitro. Subsequent molecular analysis revealed that HPX reduces intracellular reactive oxygen species levels within cultured HSCs. Furthermore, bone marrow immunostaining and 3D immunohistochemistry revealed that HPX is expressed in non-myelinating Schwann cells, known HSC niche constituents. These results highlight the utility of this fully defined all-recombinant protein-based culture system for reproducible in vitro HSC culture and its potential to contribute to the identification of factors responsible for in vitro maintenance, expansion, and differentiation of stem cell populations.

  20. Yeast hnRNP-related proteins contribute to the maintenance of telomeres

    Energy Technology Data Exchange (ETDEWEB)

    Lee-Soety, Julia Y., E-mail: jlee04@sju.edu [Department of Biology, Saint Joseph' s University, PA 19131 (United States); Jones, Jennifer; MacGibeny, Margaret A.; Remaly, Erin C.; Daniels, Lynsey; Ito, Andrea; Jean, Jessica; Radecki, Hannah; Spencer, Shannon [Department of Biology, Saint Joseph' s University, PA 19131 (United States)

    2012-09-14

    Highlights: Black-Right-Pointing-Pointer Yeast hnRNP-related proteins are able to prevent faster senescence in telomerase-null cells. Black-Right-Pointing-Pointer The conserved RRMs in Npl3 are important for telomere maintenance. Black-Right-Pointing-Pointer Human hnRNP A1 is unable to complement the lack of NPL3 in yeast. Black-Right-Pointing-Pointer Npl3 and Cbc2 may work as telomere capping proteins. -- Abstract: Telomeres protect the ends of linear chromosomes, which if eroded to a critical length can become uncapped and lead to replicative senescence. Telomerase maintains telomere length in some cells, but inappropriate expression facilitates the immortality of cancer cells. Recently, proteins involved in RNA processing and ribosome assembly, such as hnRNP (heterogeneous nuclear ribonucleoprotein) A1, have been found to participate in telomere maintenance in mammals. The Saccharomyces cerevisiae protein Npl3 shares significant amino acid sequence similarities with hnRNP A1. We found that deleting NPL3 accelerated the senescence of telomerase null cells. The highly conserved RNA recognition motifs (RRM) in Npl3 appear to be important for preventing faster senescence. Npl3 preferentially binds telomere sequences in vitro, suggesting that Npl3 may affect telomeres directly. Despite similarities between the two proteins, human hnRNP A1 is unable to complement the lack of Npl3 to rescue accelerated senescence in tlc1 npl3 cells. Deletion of CBC2, which encodes another hnRNP-related protein that associates with Npl3, also accelerates senescence. Potential mechanisms by which hnRNP-related proteins maintain telomeres are discussed.

  1. Net protein and metabolizable protein requirements for maintenance and growth of early-weaned Dorper crossbred male lambs

    Institute of Scientific and Technical Information of China (English)

    Tao Ma; Kaidong Deng; Yan Tu; Naifeng Zhang; Bingwen Si; Guishan Xu; Qiyu Diao

    2017-01-01

    Background:Dorper is an important breed for meat purpose and widely used in the livestock industry of the world.However,the protein requirement of Dorper crossbred has not been investigated.The current paper reports the net protein (NP) and metabolizable protein (MP) requirements of Dorper crossbred ram lambs from 20 to 35 kg BW.Methods:Thirty-five Dorper × thin-tailed Han crossbred lambs weaned at approximately 50 d of age (20.3 ± 2.15 kg of BW) were used.Seven lambs of 25 kg BW were slaughtered as the baseline animals at the start of the trial.An intermediate group of seven randomly selected lambs fed ad libitum was slaughtered at 28.6 kg BW.The remaining 21 lambs were randomly divided into three levels of dry matter intake:ad libitum or 70% or 40% of ad libitum intake.Those lambs were slaughtered when the lambs fed ad libitum reached 35 kg BW.Total body N and N retention were measured.Results:The daily NP and MP requirements for maintenance were 1.89 and 4.52 g/kg metabolic shrunk BW (SBW0.75).The partial efficiency of MP utilization for maintenance was 0.42.The NP requirement for growth ranged from 12.1 to 43.5 g/d,for the lambs gaining 100 to 350 g/d,and the partial efficiency of MP utilization for growth was 0.86.Conclusions:The NP and MP requirements for the maintenance and growth of Dorper crossbred male lambs were lower than the recommendations of American and British nutritional systems.

  2. Meiotic transmission of an in vitro-assembled autonomous maize minichromosome.

    Directory of Open Access Journals (Sweden)

    Shawn R Carlson

    2007-10-01

    Full Text Available Autonomous chromosomes are generated in yeast (yeast artificial chromosomes and human fibrosarcoma cells (human artificial chromosomes by introducing purified DNA fragments that nucleate a kinetochore, replicate, and segregate to daughter cells. These autonomous minichromosomes are convenient for manipulating and delivering DNA segments containing multiple genes. In contrast, commercial production of transgenic crops relies on methods that integrate one or a few genes into host chromosomes; extensive screening to identify insertions with the desired expression level, copy number, structure, and genomic location; and long breeding programs to produce varieties that carry multiple transgenes. As a step toward improving transgenic crop production, we report the development of autonomous maize minichromosomes (MMCs. We constructed circular MMCs by combining DsRed and nptII marker genes with 7-190 kb of genomic maize DNA fragments containing satellites, retroelements, and/or other repeats commonly found in centromeres and using particle bombardment to deliver these constructs into embryogenic maize tissue. We selected transformed cells, regenerated plants, and propagated their progeny for multiple generations in the absence of selection. Fluorescent in situ hybridization and segregation analysis demonstrated that autonomous MMCs can be mitotically and meiotically maintained. The MMC described here showed meiotic segregation ratios approaching Mendelian inheritance: 93% transmission as a disome (100% expected, 39% transmission as a monosome crossed to wild type (50% expected, and 59% transmission in self crosses (75% expected. The fluorescent DsRed reporter gene on the MMC was expressed through four generations, and Southern blot analysis indicated the encoded genes were intact. This novel approach for plant transformation can facilitate crop biotechnology by (i combining several trait genes on a single DNA fragment, (ii arranging genes in a defined

  3. Role of platelets in maintenance of pulmonary vascular permeability to protein

    International Nuclear Information System (INIS)

    Lo, S.K.; Burhop, K.E.; Kaplan, J.E.; Malik, A.B.

    1988-01-01

    The authors examined the role of platelets in maintenance of pulmonary vascular integrity by inducing thrombocytopenia in sheep using antiplatelet serum (APS). A causal relationship between thrombocytopenia and increase in pulmonary vascular permeability was established by platelet repletion using platelet-rich plasma (PRP). Sheep were chronically instrumented and lung lymph fistulas prepared to monitor pulmonary lymph flow (Q lym ). A balloon catheter was positioned in the left atrium to assess pulmonary vascular permeability to protein after raising the left atrial pressure (P la ). Thrombocytopenia was maintained for 3 days by daily intramuscular APS injections. In studies using cultured bovine pulmonary artery endothelial monolayers, transendothelia permeability of 125 I-labeled albumin was reduced 50 and 95%, respectively, when 2.5 x 10 7 or 5 x 10 7 platelets were added onto endothelial monolayers. However, addition of 5 x 10 6 platelets or 5 x 10 7 red blood cells did not reduce endothelial monolayer albumin permeability. Results indicate that platelets are required for the maintenance of pulmonary vascular permeability. Reduction in permeability appears to involve an interaction of platelets with the endothelium

  4. Effect of a high-protein diet on maintenance of blood pressure levels achieved after initial weight loss

    DEFF Research Database (Denmark)

    Engberink, M F; Geleijnse, J M; Bakker, S J L

    2015-01-01

    Randomized trials have shown significant blood pressure (BP) reductions after increased protein compared with carbohydrate intake, but the effect on BP maintenance after initial weight loss is unclear. We examined the effect of a high-protein diet on the maintenance of reduced BP after weight loss......(-2), BP was 118/73 mm Hg and 28 subjects (6.5%) used antihypertensive agents. Systolic BP during 26 weeks of weight maintenance dietary intervention increased in both treatment groups, but it was 2.2 mm Hg less (95% CI: -4.6 to 0.2 mm Hg, P=0.08) in the high-protein group than in the lower...

  5. Scaffolding proteins in the development and maintenance of the epidermal permeability barrier.

    Science.gov (United States)

    Crawford, Melissa; Dagnino, Lina

    2017-10-02

    The skin of mammals and other terrestrial vertebrates protects the organism against the external environment, preventing heat, water and electrolyte loss, as well as entry of chemicals and pathogens. Impairments in the epidermal permeability barrier function are associated with the genesis and/or progression of a variety of pathological conditions, including genetic inflammatory diseases, microbial and viral infections, and photodamage induced by UV radiation. In mammals, the outside-in epidermal permeability barrier is provided by the joint action of the outermost cornified layer, together with assembled tight junctions in granular keratinocytes found in the layers underneath. Tight junctions serve as both outside-in and inside-out barriers, and impede paracellular movements of ions, water, macromolecules and microorganisms. At the molecular level, tight junctions consist of integral membrane proteins that form an extracellular seal between adjacent cells, and associate with cytoplasmic scaffold proteins that serve as links with the actin cytoskeleton. In this review, we address the roles that scaffold proteins play specifically in the establishment and maintenance of the epidermal permeability barrier, and how various pathologies alter or impair their functions.

  6. Generation of an approximately 2.4 Mb human X centromere-based minichromosome by targeted telomere-associated chromosome fragmentation in DT40.

    Science.gov (United States)

    Mills, W; Critcher, R; Lee, C; Farr, C J

    1999-05-01

    A linear mammalian artificial chromosome (MAC) will require at least three types of functional element: a centromere, two telomeres and origins of replication. As yet, our understanding of these elements, as well as many other aspects of structure and organization which may be critical for a fully functional mammalian chromosome, remains poor. As a way of defining these various requirements, minichromosome reagents are being developed and analysed. Approaches for minichromosome generation fall into two broad categories: de novo assembly from candidate DNA sequences, or the fragmentation of an existing chromosome to reduce it to a minimal size. Here we describe the generation of a human minichromosome using the latter, top-down, approach. A human X chromosome, present in a DT40-human microcell hybrid, has been manipulated using homologous recombination and the targeted seeding of a de novo telomere. This strategy has generated a linear approximately 2.4 Mb human X centromere-based minichromosome capped by two artificially seeded telomeres: one immediately flanking the centromeric alpha-satellite DNA and the other targeted to the zinc finger gene ZXDA in Xp11.21. The chromosome retains an alpha-satellite domain of approximately 1. 8 Mb, a small array of gamma-satellite repeat ( approximately 40 kb) and approximately 400 kb of Xp proximal DNA sequence. The mitotic stability of this minichromosome has been examined, both in DT40 and following transfer into hamster and human cell lines. In all three backgrounds, the minichromosome is retained efficiently, but in the human and hamster microcell hybrids its copy number is poorly regulated. This approach of engineering well-defined chromosome reagents will allow key questions in MAC development (such as whether a lower size limit exists) to be addressed. In addition, the 2.4 Mb minichromosome described here has potential to be developed as a vector for gene delivery.

  7. Effect of a high-protein diet on maintenance of blood pressure levels achieved after initial weight loss : the DiOGenes randomized study

    NARCIS (Netherlands)

    Engberink, M. F.; Geleijnse, J. M.; Bakker, S. J. L.; Larsen, T. M.; Handjieva-Darlesnka, T.; Kafatos, A.; Martinez, J. A.; Pfeiffer, A. F. H.; Kunesova, M.; Jebb, S. A.; Holst, C.; Astrup, A.; Saris, W. H. M.; Brink, E. J.; van Baak, M. A.

    Randomized trials have shown significant blood pressure (BP) reductions after increased protein compared with carbohydrate intake, but the effect on BP maintenance after initial weight loss is unclear. We examined the effect of a high-protein diet on the maintenance of reduced BP after weight loss

  8. Effect of a high-protein diet on maintenance of blood pressure levels achieved after initial weight loss: the DiOGenes randomized study

    NARCIS (Netherlands)

    Engberink, M.F.; Geleijnse, J.M.; Bakker, S.J.L.; Larsen, T.

    2015-01-01

    Randomized trials have shown significant blood pressure (BP) reductions after increased protein compared with carbohydrate intake, but the effect on BP maintenance after initial weight loss is unclear. We examined the effect of a high-protein diet on the maintenance of reduced BP after weight loss

  9. Nuclear HMGA1 nonhistone chromatin proteins directly influence mitochondrial transcription, maintenance, and function

    International Nuclear Information System (INIS)

    Dement, Gregory A.; Maloney, Scott C.; Reeves, Raymond

    2007-01-01

    We have previously demonstrated that HMGA1 proteins translocate from the nucleus to mitochondria and bind to mitochondrial DNA (mtDNA) at the D-loop control region [G.A. Dement, N.R. Treff, N.S. Magnuson, V. Franceschi, R. Reeves, Dynamic mitochondrial localization of nuclear transcription factor HMGA1, Exp. Cell Res. 307 (2005) 388-401.] [11]. To elucidate possible physiological roles for such binding, we employed methods to analyze mtDNA transcription, mitochondrial maintenance, and other organelle functions in transgenic human MCF-7 cells (HA7C) induced to over-express an HA-tagged HMGA1 protein and control (parental) MCF-7 cells. Quantitative real-time (RT) PCR analyses demonstrated that mtDNA levels were reduced approximately 2-fold in HMGA1 over-expressing HA7C cells and flow cytometric analyses further revealed that mitochondrial mass was significantly reduced in these cells. Cellular ATP levels were also reduced in HA7C cells and survival studies showed an increased sensitivity to killing by 2-deoxy-D-glucose, a glycolysis-specific inhibitor. Flow cytometric analyses revealed additional mitochondrial abnormalities in HA7C cells that are consistent with a cancerous phenotype: namely, increased reactive oxygen species (ROS) and increased mitochondrial membrane potential (ΔΨ m ). Additional RT-PCR analyses demonstrated that gene transcripts from both the heavy (ND2, COXI, ATP6) and light (ND6) strands of mtDNA were up-regulated approximately 3-fold in HA7C cells. Together, these mitochondrial changes are consistent with many previous reports and reveal several possible mechanisms by which HMGA1 over-expression, a common feature of naturally occurring cancers, may affect tumor progression

  10. Protein supplements after weight loss do not improve weight maintenance compared with recommended dietary protein intake despite beneficial effects on appetite sensation and energy expenditure

    DEFF Research Database (Denmark)

    Kjølbæk, Louise; Sørensen, Lone Brinkmann; Søndertoft, Nadja Buus

    2017-01-01

    Background: High-protein diets increase weight loss (WL) during energy restriction; therefore, it has been suggested that additional protein intake may improve weight maintenance (WM) after WL.Objective: We investigated the effect of protein supplements from either whey with or without calcium...... were performed to investigate diet-induced-thermogenesis (DIT) and appetite sensation. Compliance was tested by 24-h urinary nitrogen excretion.Results: A total of 151 participants completed the WM period. The control and 3 protein supplements did not result in different mean ± SD weight regains (whey.......58 ± 1.4 kg; and control: 1.74 ± 1.4 kg; P = 0.50) during WM. Changes in blood pressure and blood biochemistry were not different between groups. Compared with the control, protein supplementation resulted in higher DIT (∼30 kJ/2.5 h) and resting energy expenditure (243 kJ/d) and an anorexigenic appetite...

  11. New method for the quality check of food proteins of the maintenance metabolism. 4. Investigation of isolated proteins as well as some protein sources of plant and animal origin

    Energy Technology Data Exchange (ETDEWEB)

    Simon, O; Hernandez, M; Bergner, H [Humboldt-Universitaet, Berlin (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin

    1981-01-01

    Male adult rats (370 g body weight) were fed on maintenance level (460 kJ ME/kgsup(0,75). In a 10 days preliminary period they received a casein/methionine (95/5) diet supplemented with 10 mg /sup 15/N excess per 0.178 kg metabolic body weight in form of ammonium acetate. Thereafter the animals were put on 8 isonitrogenous diets containing as protein sources casein, soya protein, gelatine, whole-egg, fish meal, pea, wheat and yeast. The /sup 15/N excretion via urine and feces was used to evaluate the dietary proteins for maintenance. /sup 15/N in urine was lowest in animals fed on wheat diet and highest after feeding whole-egg diet. From these data a so called '/sup 15/N excretion biological valence (BV)' was calculated, which indicated the highest quality for wheat and soy protein in meeting the needs of the intermediary maintenance metabolism. On the other hand, dietary protein sources influence the loss of endogenous nitrogen as metabolic fecal nitrogen (MFN). It was found to be lowest in animals fed on diets containing isolated proteins (6 mg MFN/100 g body weight) and highest after feeding protein sources of plant origin with a high content in crude fibre (10 mg MFN/100 g). Both, losses of /sup 15/N via urine and via feces were combined in a parameter called 'total BV'. According to this parameter the differences in quality for maintenance were only little between the protein sources tested (casein 100, soy protein 100, pea 99, wheat 99, whole egg 92, fish meal 89, gelatin 89). It was concluded that in the state of maintenance the supply with essential amino acids is not critical and that the supply with dispensable amino acids (or nonspecific nitrogen) is of great importance.

  12. Involvement of protein kinase Mζ in the maintenance of long-term memory for taste aversion learning in young chicks.

    Science.gov (United States)

    Tiunova, A A; Bezryadnov, D V; Anokhin, K V

    2015-03-01

    The effects of an inhibitor of protein kinase Mζ on long-term memory were studied using the model of taste aversion in newborn chicks. Memory was impaired by intracerebral injection of 10 or 20 nmol of ζ-inhibiting peptide 24 h after training. Memory impairment was found 2 h after peptide administration, and repeated examination 24 h after treatment showed no recovery. Memory impairment was not observed 24 h after inhibitor administration if the testing 2 h after treatment was not performed. The results indicate the contribution of protein kinase Mζ in the maintenance of long-term memory in the avian brain. These data confirm the hypothesis of several authors that inhibition of protein kinase Mζ does not abolish memory, but rather interacts with processes of memory retrieval and/or reconsolidation.

  13. Metabolic Turnover of Synaptic Proteins: Kinetics, Interdependencies and Implications for Synaptic Maintenance

    Science.gov (United States)

    Cohen, Laurie D.; Zuchman, Rina; Sorokina, Oksana; Müller, Anke; Dieterich, Daniela C.; Armstrong, J. Douglas; Ziv, Tamar; Ziv, Noam E.

    2013-01-01

    Chemical synapses contain multitudes of proteins, which in common with all proteins, have finite lifetimes and therefore need to be continuously replaced. Given the huge numbers of synaptic connections typical neurons form, the demand to maintain the protein contents of these connections might be expected to place considerable metabolic demands on each neuron. Moreover, synaptic proteostasis might differ according to distance from global protein synthesis sites, the availability of distributed protein synthesis facilities, trafficking rates and synaptic protein dynamics. To date, the turnover kinetics of synaptic proteins have not been studied or analyzed systematically, and thus metabolic demands or the aforementioned relationships remain largely unknown. In the current study we used dynamic Stable Isotope Labeling with Amino acids in Cell culture (SILAC), mass spectrometry (MS), Fluorescent Non–Canonical Amino acid Tagging (FUNCAT), quantitative immunohistochemistry and bioinformatics to systematically measure the metabolic half-lives of hundreds of synaptic proteins, examine how these depend on their pre/postsynaptic affiliation or their association with particular molecular complexes, and assess the metabolic load of synaptic proteostasis. We found that nearly all synaptic proteins identified here exhibited half-lifetimes in the range of 2–5 days. Unexpectedly, metabolic turnover rates were not significantly different for presynaptic and postsynaptic proteins, or for proteins for which mRNAs are consistently found in dendrites. Some functionally or structurally related proteins exhibited very similar turnover rates, indicating that their biogenesis and degradation might be coupled, a possibility further supported by bioinformatics-based analyses. The relatively low turnover rates measured here (∼0.7% of synaptic protein content per hour) are in good agreement with imaging-based studies of synaptic protein trafficking, yet indicate that the metabolic load

  14. The Microtubule Plus-End Tracking Protein CLASP2 Is Required for Hematopoiesis and Hematopoietic Stem Cell Maintenance

    Directory of Open Access Journals (Sweden)

    Ksenija Drabek

    2012-10-01

    Full Text Available Mammalian CLASPs are microtubule plus-end tracking proteins whose essential function as regulators of microtubule behavior has been studied mainly in cultured cells. We show here that absence of murine CLASP2 in vivo results in thrombocytopenia, progressive anemia, and pancytopenia, due to defects in megakaryopoiesis, in erythropoiesis, and in the maintenance of hematopoietic stem cell activity. Furthermore, microtubule stability and organization are affected upon attachment of Clasp2 knockout hematopoietic stem-cell-enriched populations, and these cells do not home efficiently toward their bone marrow niche. Strikingly, CLASP2-deficient hematopoietic stem cells contain severely reduced mRNA levels of c-Mpl, which encodes the thrombopoietin receptor, an essential factor for megakaryopoiesis and hematopoietic stem cell maintenance. Our data suggest that thrombopoietin signaling is impaired in Clasp2 knockout mice. We propose that the CLASP2-mediated stabilization of microtubules is required for proper attachment, homing, and maintenance of hematopoietic stem cells and that this is necessary to sustain c-Mpl transcription.

  15. Getting to the meat of the matter: beyond protein supplementation in maintenance dialysis.

    Science.gov (United States)

    Bailey, James L; Franch, Harold A

    2009-01-01

    Until recently, patients on dialysis with low serum albumin levels were characterized as suffering from protein malnutrition suggesting that the cause of this malady was due to an inadequate intake of protein. In fact, these patients tend to suffer from a wasting syndrome similar to cachexia commonly associated with inflammation in which there is loss of lean body mass and fat mass is underutilized. The term protein energy wasting has been used to characterize this syndrome and suggests that the simple addition of protein supplements to the dietary regimen of hemodialysis patients will not cure this malady. Correction of the underlying inflammatory disorder which drives losses of body protein and fuel reserves is far more important and is the single most effective therapy. Protein supplements which may promote albumin synthesis and synthesis of liver-related proteins tend to increase muscle catabolism. Muscle growth is not fostered by increasing dietary protein above recommended goals for dialysis patients, but can be promoted by the addition of protein of high biological value that is rich in leucine and other essential amino acids in tandem with repetitive exercises. Ultimately, correction of PEW hinges on the diagnosis and treatment of co-morbid conditions in combination with strategies to replenish caloric and protein stores. A supplementary exercise program would allow recovery of lean body mass. Given the multiple co-morbidities that exist in this population, therapy would have to be individualized.

  16. Effect of short-term low-protein diet supplemented with keto acids on hyperphosphatemia in maintenance hemodialysis patients.

    Science.gov (United States)

    Li, Haiming; Long, Quan; Shao, Chunhai; Fan, Hong; Yuan, Li; Huang, Bihong; Gu, Yong; Lin, Shanyan; Hao, Chuanming; Chen, Jing

    2011-01-01

    To evaluate the effects of short-term restriction of dietary protein intake (DPI) supplemented with keto acids on hyperphosphatemia in maintenance hemodialysis (MHD) patients. Forty MHD patients with uncontrolled hyperphosphatemia were randomized to either low DPI with keto acid-supplemented (sLP) or normal DPI (NP) group for 8 weeks. After 8 weeks, the sLP group was shifted to NP for another 8 weeks. Low-protein diet (LPD) was individualized with total caloric intake 30-35 kcal/kg/day, protein intake of 0.8 g/kg/day and phosphate intake of 500 mg/day. Keto acids were supplied in a dosage of 12 pills per day. Calcium phosphorous metabolism index and nutritional index (serum albumin, total protein, somatometric measurements, 3-day diaries and Mini-Nutritional Assessment score) were recorded. C-reactive protein, CO(2) combining power and Kt/V were measured to evaluate the inflammation, metabolic acidosis and dialysis adequacy, respectively. Serum phosphorus level and calcium-phosphate product were significantly decreased at the end of the first 8 weeks in the sLP group compared to the basal value and the NP group (p keto acids could decrease hyperphosphatemia and calcium-phosphate product, while keeping stable nutritional status among MHD patients. Copyright © 2010 S. Karger AG, Basel.

  17. Diets with high or low protein content and glycemic index for weight-loss maintenance

    DEFF Research Database (Denmark)

    Larsen, Thomas Meinert; Dalskov, Stine-Mathilde; Baak, Marleen van

    2010-01-01

    Studies of weight-control diets that are high in protein or low in glycemic index have reached varied conclusions, probably owing to the fact that the studies had insufficient power.......Studies of weight-control diets that are high in protein or low in glycemic index have reached varied conclusions, probably owing to the fact that the studies had insufficient power....

  18. The MCM-associated protein MCM-BP is important for human nuclear morphology.

    Science.gov (United States)

    Jagannathan, Madhav; Sakwe, Amos M; Nguyen, Tin; Frappier, Lori

    2012-01-01

    Mini-chromosome maintenance complex-binding protein (MCM-BP) was discovered as a protein that is strongly associated with human MCM proteins, known to be crucial for DNA replication in providing DNA helicase activity. The Xenopus MCM-BP homologue appears to play a role in unloading MCM complexes from chromatin after DNA synthesis; however, the importance of MCM-BP and its functional contribution to human cells has been unclear. Here we show that depletion of MCM-BP by sustained expression of short hairpin RNA (shRNA) results in highly abnormal nuclear morphology and centrosome amplification. The abnormal nuclear morphology was not seen with depletion of other MCM proteins and was rescued with shRNA-resistant MCM-BP. MCM-BP depletion was also found to result in transient activation of the G2 checkpoint, slowed progression through G2 and increased replication protein A foci, indicative of replication stress. In addition, MCM-BP depletion led to increased cellular levels of MCM proteins throughout the cell cycle including soluble MCM pools. The results suggest that MCM-BP makes multiple contributions to human cells that are not limited to unloading of the MCM complex.

  19. Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload

    Science.gov (United States)

    Kuipers, Marjorie A.; Stasevich, Timothy J.; Sasaki, Takayo; Wilson, Korey A.; Hazelwood, Kristin L.; McNally, James G.; Davidson, Michael W.

    2011-01-01

    The heterohexameric minichromosome maintenance protein complex (Mcm2-7) functions as the eukaryotic helicase during DNA replication. Mcm2-7 loads onto chromatin during early G1 phase but is not converted into an active helicase until much later during S phase. Hence, inactive Mcm complexes are presumed to remain stably bound from early G1 through the completion of S phase. Here, we investigated Mcm protein dynamics in live mammalian cells. We demonstrate that Mcm proteins are irreversibly loaded onto chromatin cumulatively throughout G1 phase, showing no detectable exchange with a gradually diminishing soluble pool. Eviction of Mcm requires replication; during replication arrest, Mcm proteins remained bound indefinitely. Moreover, the density of immobile Mcms is reduced together with chromatin decondensation within sites of active replication, which provides an explanation for the lack of colocalization of Mcm with replication fork proteins. These results provide in vivo evidence for an exceptionally stable lockdown mechanism to retain all loaded Mcm proteins on chromatin throughout prolonged cell cycles. PMID:21220507

  20. Long term weight maintenance after advice to consume low carbohydrate, higher protein diets--a systematic review and meta analysis.

    Science.gov (United States)

    Clifton, P M; Condo, D; Keogh, J B

    2014-03-01

    Meta analysis of short term trials indicates that a higher protein, lower carbohydrate weight loss diet enhances fat mass loss and limits lean mass loss compared with a normal protein weight loss diet. Whether this benefit persists long term is not clear. We selected weight loss studies in adults with at least a 12 month follow up in which a higher percentage protein/lower carbohydrate diet was either planned or would be expected for either weight loss or weight maintenance. Studies were selected regardless of the success of the advice but difference in absolute and percentage protein intake at 12 months was used as a moderator in the analysis. Data was analysed using Comprehensive Meta analysis V2 using a random effects analysis. As many as 32 studies with 3492 individuals were analysed with data on fat and lean mass, glucose and insulin from 18 to 22 studies and lipids from 28 studies. A recommendation to consume a lower carbohydrate, higher protein diet in mostly short term intensive interventions with long term follow up was associated with better weight and fat loss but the effect size was small-standardised means of 0.14 and 0.22, p = 0.008 and p fat mass (0.9 vs. 0.3 kg). Fasting triglyceride and insulin were also lower with high protein diets with effect sizes of 0.17 and 0.22, p = 0.003 and p = 0.042 respectively. Other lipids and glucose were not different. The short term benefit of higher protein diets appears to persist to a small degree long term. Benefits are greater with better compliance to the diet. Copyright © 2013 Elsevier B.V. All rights reserved.

  1. ngs (notochord granular surface) gene encodes a novel type of intermediate filament family protein essential for notochord maintenance in zebrafish.

    Science.gov (United States)

    Tong, Xiangjun; Xia, Zhidan; Zu, Yao; Telfer, Helena; Hu, Jing; Yu, Jingyi; Liu, Huan; Zhang, Quan; Sodmergen; Lin, Shuo; Zhang, Bo

    2013-01-25

    The notochord is an important organ involved in embryonic patterning and locomotion. In zebrafish, the mature notochord consists of a single stack of fully differentiated, large vacuolated cells called chordocytes, surrounded by a single layer of less differentiated notochordal epithelial cells called chordoblasts. Through genetic analysis of zebrafish lines carrying pseudo-typed retroviral insertions, a mutant exhibiting a defective notochord with a granular appearance was isolated, and the corresponding gene was identified as ngs (notochord granular surface), which was specifically expressed in the notochord. In the mutants, the notochord started to degenerate from 32 hours post-fertilization, and the chordocytes were then gradually replaced by smaller cells derived from chordoblasts. The granular notochord phenotype was alleviated by anesthetizing the mutant embryos with tricaine to prevent muscle contraction and locomotion. Phylogenetic analysis showed that ngs encodes a new type of intermediate filament (IF) family protein, which we named chordostatin based on its function. Under the transmission electron microcopy, bundles of 10-nm-thick IF-like filaments were enriched in the chordocytes of wild-type zebrafish embryos, whereas the chordocytes in ngs mutants lacked IF-like structures. Furthermore, chordostatin-enhanced GFP (EGFP) fusion protein assembled into a filamentous network specifically in chordocytes. Taken together, our work demonstrates that ngs encodes a novel type of IF protein and functions to maintain notochord integrity for larval development and locomotion. Our work sheds light on the mechanisms of notochord structural maintenance, as well as the evolution and biological function of IF family proteins.

  2. Apical Polarity Protein PrkCi Is Necessary for Maintenance of Spinal Cord Precursors in Zebrafish

    OpenAIRE

    Roberts, Randolph K.; Appel, Bruce

    2009-01-01

    During development, neural precursors divide to produce new precursors and cells that differentiate as neurons and glia. In Drosophila, apicobasal polarity and orientation of the mitotic spindle play important roles in specifying the progeny of neural precursors for different fates. We examined orientation of zebrafish spinal cord precursors using time-lapse imaging and tested the function of protein kinase C, iota (PrkCi), a member of the Par complex of proteins necessary for apicobasal pola...

  3. Key challenges for the creation and maintenance of specialist protein resources

    Science.gov (United States)

    Holliday, Gemma L; Bairoch, Amos; Bagos, Pantelis G; Chatonnet, Arnaud; Craik, David J; Finn, Robert D; Henrissat, Bernard; Landsman, David; Manning, Gerard; Nagano, Nozomi; O’Donovan, Claire; Pruitt, Kim D; Rawlings, Neil D; Saier, Milton; Sowdhamini, Ramanathan; Spedding, Michael; Srinivasan, Narayanaswamy; Vriend, Gert; Babbitt, Patricia C; Bateman, Alex

    2015-01-01

    As the volume of data relating to proteins increases, researchers rely more and more on the analysis of published data, thus increasing the importance of good access to these data that vary from the supplemental material of individual articles, all the way to major reference databases with professional staff and long-term funding. Specialist protein resources fill an important middle ground, providing interactive web interfaces to their databases for a focused topic or family of proteins, using specialized approaches that are not feasible in the major reference databases. Many are labors of love, run by a single lab with little or no dedicated funding and there are many challenges to building and maintaining them. This perspective arose from a meeting of several specialist protein resources and major reference databases held at the Wellcome Trust Genome Campus (Cambridge, UK) on August 11 and 12, 2014. During this meeting some common key challenges involved in creating and maintaining such resources were discussed, along with various approaches to address them. In laying out these challenges, we aim to inform users about how these issues impact our resources and illustrate ways in which our working together could enhance their accuracy, currency, and overall value. Proteins 2015; 83:1005–1013. © 2015 The Authors. Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc. PMID:25820941

  4. Relatively high-protein or 'low-carb' energy-restricted diets for body weight loss and body weight maintenance?

    Science.gov (United States)

    Soenen, Stijn; Bonomi, Alberto G; Lemmens, Sofie G T; Scholte, Jolande; Thijssen, Myriam A M A; van Berkum, Frank; Westerterp-Plantenga, Margriet S

    2012-10-10

    'Low-carb' diets have been suggested to be effective in body weight (BW) management. However, these diets are relatively high in protein as well. To unravel whether body-weight loss and weight-maintenance depends on the high-protein or the 'low-carb' component of the diet. Body-weight (BW), fat mass (FM), blood- and urine-parameters of 132 participants (age=50 ± 12 yr; BW=107 ± 20 kg; BMI=37 ± 6 kg/m(2); FM=47.5 ± 11.9 kg) were compared after 3 and 12 months between four energy-restricted diets with 33% of energy requirement for the first 3 months, and 67% for the last 9 months: normal-protein normal-carbohydrate (NPNC), normal-protein low-carbohydrate (NPLC); high-protein normal-carbohydrate (HPNC), high-protein low-carbohydrate (HPLC); 24h N-analyses confirmed daily protein intakes for the normal-protein diets of 0.7 ± 0.1 and for the high-protein diets of 1.1 ± 0.2g/kg BW (pvs. NP (-11.5 ± 4 kg; -9.3 ± 0.7 kg) (pvs. NC (-12.3 ± 3 kg; -10.3 ± 1.1 kg) (ns). Diet × time interaction showed HPLC (-14.7 ± 5 kg; -11.9 ± 1.6 kg) vs. HPNC (-13.8 ± 3 kg; -11.9 ± 1.8 kg) (ns); NPLC (-12.2 ± 4 kg; -10.0 ± 0.8 kg) vs. NPNC (-10.7 ± 4 kg; -8.6 ± 0.7 kg) (ns); HPLC vs. NPLC (pvs. NPNC (pvs. NP (-8.9 ± 3 kg; -7.7 ± 0.6 kg) (pvs. NC (11.1 ± 3 kg; 9.3 ± 0.7 kg) (ns). Diet × time interaction showed HPLC (-11.6 ± 5 kg ; -8.2 ± 0.7 kg) vs. HPNC (-14.1 ± 4 kg; -10.0 ± 0.9 kg) (ns); NPNC (-8.2 ± 3 kg; -6.7 ± 0.6 kg) vs. NPLC (-9.7 ± 3 kg; -8.5 ± 0.7 kg) (ns); HPLC vs. NPLC (pvs. NPNC (pvs. all other diets reduced diastolic blood pressure more. Relationships between changes in BW, FM, FFM or metabolic parameters and energy percentage of fat in the diet were not statistically significant. Metabolic profile and fat-free-mass were improved following weight-loss. Body-weight loss and weight-maintenance depends on the high-protein, but not on the 'low-carb' component of the diet, while it is unrelated to the concomitant fat-content of the diet. Copyright

  5. Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication

    Energy Technology Data Exchange (ETDEWEB)

    Kanginakudru, Sriramana, E-mail: skangina@iu.edu [Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN (United States); DeSmet, Marsha, E-mail: mdesmet@iupui.edu [Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN (United States); Thomas, Yanique, E-mail: ysthomas@umail.iu.edu [Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN (United States); Morgan, Iain M., E-mail: immorgan@vcu.edu [VCU Philips Institute for Oral Health Research, Virginia Commonwealth University, Richmond, Virginia (United States); Androphy, Elliot J., E-mail: eandro@iu.edu [Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN (United States); Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN (United States)

    2015-04-15

    The evolutionarily conserved DNA topoisomerase II beta-binding protein 1 (TopBP1) functions in DNA replication, DNA damage response, and cell survival. We analyzed the role of TopBP1 in human and bovine papillomavirus genome replication. Consistent with prior reports, TopBP1 co-localized in discrete nuclear foci and was in complex with papillomavirus E2 protein. Similar to E2, TopBP1 is recruited to the region of the viral origin of replication during G1/S and early S phase. TopBP1 knockdown increased, while over-expression decreased transient virus replication, without affecting cell cycle. Similarly, using cell lines harboring HPV-16 or HPV-31 genome, TopBP1 knockdown increased while over-expression reduced viral copy number relative to genomic DNA. We propose a model in which TopBP1 serves dual roles in viral replication: it is essential for initiation of replication yet it restricts viral copy number. - Highlights: • Protein interaction study confirmed In-situ interaction between TopBP1 and E2. • TopBP1 present at papillomavirus ori in G1/S and early S phase of cell cycle. • TopBP1 knockdown increased, over-expression reduced virus replication. • TopBP1 protein level change did not influence cell survival or cell cycle. • TopBP1 displaced from papillomavirus ori after initiation of replication.

  6. Levels of the E2 interacting protein TopBP1 modulate papillomavirus maintenance stage replication

    International Nuclear Information System (INIS)

    Kanginakudru, Sriramana; DeSmet, Marsha; Thomas, Yanique; Morgan, Iain M.; Androphy, Elliot J.

    2015-01-01

    The evolutionarily conserved DNA topoisomerase II beta-binding protein 1 (TopBP1) functions in DNA replication, DNA damage response, and cell survival. We analyzed the role of TopBP1 in human and bovine papillomavirus genome replication. Consistent with prior reports, TopBP1 co-localized in discrete nuclear foci and was in complex with papillomavirus E2 protein. Similar to E2, TopBP1 is recruited to the region of the viral origin of replication during G1/S and early S phase. TopBP1 knockdown increased, while over-expression decreased transient virus replication, without affecting cell cycle. Similarly, using cell lines harboring HPV-16 or HPV-31 genome, TopBP1 knockdown increased while over-expression reduced viral copy number relative to genomic DNA. We propose a model in which TopBP1 serves dual roles in viral replication: it is essential for initiation of replication yet it restricts viral copy number. - Highlights: • Protein interaction study confirmed In-situ interaction between TopBP1 and E2. • TopBP1 present at papillomavirus ori in G1/S and early S phase of cell cycle. • TopBP1 knockdown increased, over-expression reduced virus replication. • TopBP1 protein level change did not influence cell survival or cell cycle. • TopBP1 displaced from papillomavirus ori after initiation of replication

  7. Key challenges for the creation and maintenance of specialist protein resources

    NARCIS (Netherlands)

    Holliday, G.L.; Bairoch, A.; Bagos, P.G.; Chatonnet, A.; Craik, D.J.; Finn, R.D.; Henrissat, B.; Landsman, D.; Manning, G.; Nagano, N.; O'Donovan, C.; Pruitt, K.D.; Rawlings, N.D.; Saier, M.; Sowdhamini, R.; Spedding, M.; Srinivasan, N.; Vriend, G.; Babbitt, P.C.; Bateman, A.

    2015-01-01

    As the volume of data relating to proteins increases, researchers rely more and more on the analysis of published data, thus increasing the importance of good access to these data that vary from the supplemental material of individual articles, all the way to major reference databases with

  8. The impact of gender and protein intake on the success of weight maintenance and associated cardiovascular risk benefits, independent of the mode of food provision

    DEFF Research Database (Denmark)

    Navas-Carretero, Santiago; Holst, Claus; Saris, Wim H

    2016-01-01

    OBJECTIVE: Maintenance of weight loss and associated cardiovascular benefits after following energy-restricted diets is still a challenging field, and thorough investigation is needed. The present research aimed to determine the role of protein and gender in relation to two different intervention...... models related to food supply, in a weight maintenance trial. SUBJECTS AND METHODS: The DiOGenes trial was a long-term, multicenter, randomized, dietary intervention study, conducted in eight European countries (Clinical Trials.gov, NCT00390637), focusing on assessing the effectiveness of weight...... and macronutrient intake. RESULTS: The weight maintenance intervention based on different macronutrient intake showed, independently of the advice delivery model, in both sexes that higher protein consumption was more effective for weight stability, showing better results in obese women (low protein: 1.65 kg...

  9. Protein synthesis is essential not only for consolidation but also for maintenance and post-retrieval reconsolidation of acrobatic motor skill in rats.

    Science.gov (United States)

    Peng, Ji-Yun; Li, Bao-Ming

    2009-05-28

    It has been reported that consolidation of motor skill, a type of non-declarative memories, requires protein synthesis, as hippocampus-dependent declarative memory does. However, little is known about the importance of protein synthesis in maintenance and especially post-retrieval reconsolidation of acrobatic motor skill. Here, we show that protein synthesis is essential not only for the consolidation but also for the maintenance and reconsolidation of a rotarod-running skill. Intra-ventricle infusion of the protein synthesis inhibitor anisomycin 0 h but not 2 h post-training caused a severe deficit in the acquisition of the rotarod-running skill. Protein synthesis inhibition (PSI) also caused a deficit in the maintenance of the rotarod-running skill, as well-trained rats demonstrated a deficit in the rotarod-running performance upon treatment with anisomycin. Similarly, PSI impaired the post-retrieval reconsolidation of the rotarod-running skill: well-trained rats treated with anisomycin 0 h but not 0.5, 2 and 4 h after the task performance exhibited amnesia for the running skill later on. Interestingly, rats treated with anisomycin 6 and 12 h post-retrieval exhibited amnesia for the running skill. Thus, protein synthesis is essential not only for the consolidation but also for the maintenance and post-retrieval reconsolidation of rotarod-running acrobatic motor skill.

  10. Protein synthesis is essential not only for consolidation but also for maintenance and post-retrieval reconsolidation of acrobatic motor skill in rats

    Directory of Open Access Journals (Sweden)

    Peng Ji-Yun

    2009-05-01

    Full Text Available Abstract It has been reported that consolidation of motor skill, a type of non-declarative memories, requires protein synthesis, as hippocampus-dependent declarative memory does. However, little is known about the importance of protein synthesis in maintenance and especially post-retrieval reconsolidation of acrobatic motor skill. Here, we show that protein synthesis is essential not only for the consolidation but also for the maintenance and reconsolidation of a rotarod-running skill. Intra-ventricle infusion of the protein synthesis inhibitor anisomycin 0 h but not 2 h post-training caused a severe deficit in the acquisition of the rotarod-running skill. Protein synthesis inhibition (PSI also caused a deficit in the maintenance of the rotarod-running skill, as well-trained rats demonstrated a deficit in the rotarod-running performance upon treatment with anisomycin. Similarly, PSI impaired the post-retrieval reconsolidation of the rotarod-running skill: well-trained rats treated with anisomycin 0 h but not 0.5, 2 and 4 h after the task performance exhibited amnesia for the running skill later on. Interestingly, rats treated with anisomycin 6 and 12 h post-retrieval exhibited amnesia for the running skill. Thus, protein synthesis is essential not only for the consolidation but also for the maintenance and post-retrieval reconsolidation of rotarod-running acrobatic motor skill.

  11. Maintenance energy requirements of beef cows and relationship with cow and calf performance, metabolic hormones, and functional proteins.

    Science.gov (United States)

    Cooper-Prado, M J; Long, N M; Davis, M P; Wright, E C; Madden, R D; Dilwith, J W; Bailey, C L; Spicer, L J; Wettemann, R P

    2014-08-01

    Gestating Angus, nonlactating, spring-calving cows were used to determine variation in maintenance energy requirements (MR); to evaluate the relationship among MR and cow and calf performance, plasma concentrations of IGF-I, T4, glucose, insulin, and ruminal temperature; and to describe the LM proteome and evaluate protein abundance in cows with different MR. Cows (4 to 7 yr of age) with a BCS of 5.0 ± 0.2 and BW of 582 ± 37 kg in the second to third trimester of gestation were studied in 3 trials (trial 1, n = 23; trial 2, n = 32; trial 3, n = 38). Cows were individually fed a complete diet in amounts to meet predicted MR (Level 1 Model of NRC), and feed intake was adjusted weekly until constant BW was achieved for at least 21 d (maintenance). Cows were classified on the basis of MR as low (>0.5 SD less than mean, LMR), moderate (±0.5 SD of mean, MMR), or high (>0.5 SD more than mean, HMR) MR. Blood samples were taken at maintenance and at 2 mo postpartum in trial 2. Muscle biopsies were taken from LMR and HMR after cows consumed actual MR for 28 d (trial 2) or 21 d (trial 3). Proteins from LM were separated by 2-dimensional difference gel electrophoresis and were identified, and abundance was quantified and compared. The greatest differences in MR between cows were 29%, 24%, and 25% in trials 1, 2, and 3, respectively. Daily MR (NEm, kcal·BW(-0.75)·d(-1)) averaged 89.2 ± 6.3, 93.0 ± 4.9, and 90.4 ± 4.6 in trials 1, 2, and 3, respectively. Postpartum BW and BCS, calf birth and weaning weights, postpartum luteal activity, and ruminal temperature were not influenced by MR of the cows. Concentrations of IGF-I were greater (P = 0.001) in plasma of MMR compared with LMR cows consuming predicted MR diets, and MR was negatively correlated with concentrations of IGF-I in plasma (r = -0.38; P = 0.05) at 2 mo postpartum. A total of 103 proteins were isolated from LM; 52 gene products were identified. Abundance of specific proteins in the LM was not influenced (P > 0

  12. Roles of conceptus secretory proteins in establishment and maintenance of pregnancy in ruminants.

    Science.gov (United States)

    Bazer, Fuller W; Song, Gwonhwa; Thatcher, William W

    2012-01-01

    Reproduction in ruminant species is a highly complex biological process requiring a dialogue between the developing conceptus (embryo-fetus and associated placental membranes) and maternal uterus which must be established during the peri-implantation period for pregnancy recognition signaling and regulation of gene expression by uterine epithelial and stromal cells. The uterus provide a microenvironment in which molecules secreted by uterine epithelia and transported into the uterine lumen represent histotroph, also known as the secretome, that are required for growth and development of the conceptus and receptivity of the uterus to implantation by the elongating conceptus. Pregnancy recognition signaling as related to sustaining the functional lifespan of the corpora lutea, is required to sustain the functional life-span of corpora lutea for production of progesterone which is essential for uterine functions supportive of implantation and placentation required for successful outcomes of pregnancy. It is within the peri-implantation period that most embryonic deaths occur in ruminants due to deficiencies attributed to uterine functions or failure of the conceptus to develop appropriately, signal pregnancy recognition and/or undergo implantation and placentation. The endocrine status of the pregnant ruminant and her nutritional status are critical for successful establishment and maintenance of pregnancy. The challenge is to understand the complexity of key mechanisms that are characteristic of successful reproduction in humans and animals and to use that knowledge to enhance fertility and reproductive health of ruminant species in livestock enterprises.

  13. TFAP2B -Dietary Protein and Glycemic Index Interactions and Weight Maintenance after Weight Loss in the DiOGenes Trial

    DEFF Research Database (Denmark)

    Stocks, Tanja; Ängquist, Lars Henrik; Hager, Jörg

    2013-01-01

    Background: TFAP2B rs987237 is associated with obesity and has shown interaction with the dietary fat-to-carbohydrate ratio, which has an effect on weight loss. We investigated interactions between rs987237 and protein-to-carbohydrate ratio or glycemic index (GI) in relation to weight maintenance...... percentage from fat: either low-protein/low-GI, low-protein/high-GI, high-protein/low-GI, or high-protein/high-GI diets, or a control diet for a 6-month weight maintenance period. Using linear regression analyses and additive genetic models, we investigated main and dietary interaction effects of TFAP2B rs...... diverge depending on the nutritional state. © 2013 S. Karger AG, Basel....

  14. DNA2—An Important Player in DNA Damage Response or Just Another DNA Maintenance Protein?

    Directory of Open Access Journals (Sweden)

    Elzbieta Pawłowska

    2017-07-01

    Full Text Available The human DNA2 (DNA replication helicase/nuclease 2 protein is expressed in both the nucleus and mitochondria, where it displays ATPase-dependent nuclease and helicase activities. DNA2 plays an important role in the removing of long flaps in DNA replication and long-patch base excision repair (LP-BER, interacting with the replication protein A (RPA and the flap endonuclease 1 (FEN1. DNA2 can promote the restart of arrested replication fork along with Werner syndrome ATP-dependent helicase (WRN and Bloom syndrome protein (BLM. In mitochondria, DNA2 can facilitate primer removal during strand-displacement replication. DNA2 is involved in DNA double strand (DSB repair, in which it is complexed with BLM, RPA and MRN for DNA strand resection required for homologous recombination repair. DNA2 can be a major protein involved in the repair of complex DNA damage containing a DSB and a 5′ adduct resulting from a chemical group bound to DNA 5′ ends, created by ionizing radiation and several anticancer drugs, including etoposide, mitoxantrone and some anthracyclines. The role of DNA2 in telomere end maintenance and cell cycle regulation suggests its more general role in keeping genomic stability, which is impaired in cancer. Therefore DNA2 can be an attractive target in cancer therapy. This is supported by enhanced expression of DNA2 in many cancer cell lines with oncogene activation and premalignant cells. Therefore, DNA2 can be considered as a potential marker, useful in cancer therapy. DNA2, along with PARP1 inhibition, may be considered as a potential target for inducing synthetic lethality, a concept of killing tumor cells by targeting two essential genes.

  15. ngs (Notochord Granular Surface) Gene Encodes a Novel Type of Intermediate Filament Family Protein Essential for Notochord Maintenance in Zebrafish*

    Science.gov (United States)

    Tong, Xiangjun; Xia, Zhidan; Zu, Yao; Telfer, Helena; Hu, Jing; Yu, Jingyi; Liu, Huan; Zhang, Quan; Sodmergen; Lin, Shuo; Zhang, Bo

    2013-01-01

    The notochord is an important organ involved in embryonic patterning and locomotion. In zebrafish, the mature notochord consists of a single stack of fully differentiated, large vacuolated cells called chordocytes, surrounded by a single layer of less differentiated notochordal epithelial cells called chordoblasts. Through genetic analysis of zebrafish lines carrying pseudo-typed retroviral insertions, a mutant exhibiting a defective notochord with a granular appearance was isolated, and the corresponding gene was identified as ngs (notochord granular surface), which was specifically expressed in the notochord. In the mutants, the notochord started to degenerate from 32 hours post-fertilization, and the chordocytes were then gradually replaced by smaller cells derived from chordoblasts. The granular notochord phenotype was alleviated by anesthetizing the mutant embryos with tricaine to prevent muscle contraction and locomotion. Phylogenetic analysis showed that ngs encodes a new type of intermediate filament (IF) family protein, which we named chordostatin based on its function. Under the transmission electron microcopy, bundles of 10-nm-thick IF-like filaments were enriched in the chordocytes of wild-type zebrafish embryos, whereas the chordocytes in ngs mutants lacked IF-like structures. Furthermore, chordostatin-enhanced GFP (EGFP) fusion protein assembled into a filamentous network specifically in chordocytes. Taken together, our work demonstrates that ngs encodes a novel type of IF protein and functions to maintain notochord integrity for larval development and locomotion. Our work sheds light on the mechanisms of notochord structural maintenance, as well as the evolution and biological function of IF family proteins. PMID:23132861

  16. Evolution of DNA replication protein complexes in eukaryotes and Archaea.

    Directory of Open Access Journals (Sweden)

    Nicholas Chia

    Full Text Available BACKGROUND: The replication of DNA in Archaea and eukaryotes requires several ancillary complexes, including proliferating cell nuclear antigen (PCNA, replication factor C (RFC, and the minichromosome maintenance (MCM complex. Bacterial DNA replication utilizes comparable proteins, but these are distantly related phylogenetically to their archaeal and eukaryotic counterparts at best. METHODOLOGY/PRINCIPAL FINDINGS: While the structures of each of the complexes do not differ significantly between the archaeal and eukaryotic versions thereof, the evolutionary dynamic in the two cases does. The number of subunits in each complex is constant across all taxa. However, they vary subtly with regard to composition. In some taxa the subunits are all identical in sequence, while in others some are homologous rather than identical. In the case of eukaryotes, there is no phylogenetic variation in the makeup of each complex-all appear to derive from a common eukaryotic ancestor. This is not the case in Archaea, where the relationship between the subunits within each complex varies taxon-to-taxon. We have performed a detailed phylogenetic analysis of these relationships in order to better understand the gene duplications and divergences that gave rise to the homologous subunits in Archaea. CONCLUSION/SIGNIFICANCE: This domain level difference in evolution suggests that different forces have driven the evolution of DNA replication proteins in each of these two domains. In addition, the phylogenies of all three gene families support the distinctiveness of the proposed archaeal phylum Thaumarchaeota.

  17. Nutritional Status of Maintenance Dialysis Patients: Low Lean Body Mass Index and Obesity Are Common, Protein-Energy Wasting Is Uncommon.

    Directory of Open Access Journals (Sweden)

    Mette Koefoed

    Full Text Available Maintenance dialysis patients are at increased risk of abnormal nutritional status due to numerous causative factors, both nutritional and non-nutritional. The present study assessed the current prevalence of protein-energy wasting, low lean body mass index and obesity in maintenance dialysis patients, and compared different methods of nutritional assessment.In a cross-sectional study conducted in 2014 at Roskilde Hospital, Denmark, we performed anthropometry (body weight, skinfolds, mid-arm, waist, and hip circumferences, and determined plasma albumin and normalized protein catabolic rate in order to assess the prevalence of protein-energy wasting, low lean body mass index and obesity in these patients.Seventy-nine eligible maintenance dialysis patients participated. The prevalence of protein-energy wasted patients was 4% (95% CI: 2-12 as assessed by the coexistence of low lean body mass index and low fat mass index. Low lean body mass index was seen in 32% (95% CI: 22-44. Obesity prevalence as assessed from fat mass index was 43% (95% CI: 32-55. Coexistence of low lean body mass index and obesity was seen in 10% (95% CI: 5-19. The prevalence of protein-energy wasting and obesity varied considerably, depending on nutritional assessment methodology.Our data indicate that protein-energy wasting is uncommon, whereas low lean body mass index and obesity are frequent conditions among patients in maintenance dialysis. A focus on how to increase and preserve lean body mass in dialysis patients is suggested in the future. In order to clearly distinguish between shortage, sufficiency and abundance of protein and/or fat deposits in maintenance dialysis patients, we suggest the simple measurements of lean body mass index and fat mass index.

  18. Nutritional Status of Maintenance Dialysis Patients: Low Lean Body Mass Index and Obesity Are Common, Protein-Energy Wasting Is Uncommon.

    Science.gov (United States)

    Koefoed, Mette; Kromann, Charles Boy; Juliussen, Sophie Ryberg; Hvidtfeldt, Danni; Ekelund, Bo; Frandsen, Niels Erik; Marckmann, Peter

    2016-01-01

    Maintenance dialysis patients are at increased risk of abnormal nutritional status due to numerous causative factors, both nutritional and non-nutritional. The present study assessed the current prevalence of protein-energy wasting, low lean body mass index and obesity in maintenance dialysis patients, and compared different methods of nutritional assessment. In a cross-sectional study conducted in 2014 at Roskilde Hospital, Denmark, we performed anthropometry (body weight, skinfolds, mid-arm, waist, and hip circumferences), and determined plasma albumin and normalized protein catabolic rate in order to assess the prevalence of protein-energy wasting, low lean body mass index and obesity in these patients. Seventy-nine eligible maintenance dialysis patients participated. The prevalence of protein-energy wasted patients was 4% (95% CI: 2-12) as assessed by the coexistence of low lean body mass index and low fat mass index. Low lean body mass index was seen in 32% (95% CI: 22-44). Obesity prevalence as assessed from fat mass index was 43% (95% CI: 32-55). Coexistence of low lean body mass index and obesity was seen in 10% (95% CI: 5-19). The prevalence of protein-energy wasting and obesity varied considerably, depending on nutritional assessment methodology. Our data indicate that protein-energy wasting is uncommon, whereas low lean body mass index and obesity are frequent conditions among patients in maintenance dialysis. A focus on how to increase and preserve lean body mass in dialysis patients is suggested in the future. In order to clearly distinguish between shortage, sufficiency and abundance of protein and/or fat deposits in maintenance dialysis patients, we suggest the simple measurements of lean body mass index and fat mass index.

  19. Effect of nonsurgical periodontal treatment on C-reactive protein levels in maintenance hemodialysis patients.

    Science.gov (United States)

    Yazdi, Farin Kiany; Karimi, Noozhan; Rasouli, Manoochehr; Roozbeh, Jamshid

    2013-01-01

    C-reactive protein (CRP) has been implicated as a possible mediator of the association between periodontitis and several systemic diseases. This study evaluated the impact of nonsurgical periodontal treatment on the serum levels of CRP in chronic kidney disease (CKD) patients on hemodialysis. A total of 77 CKD patients on hemodialysis were included in this study. At baseline, periodontal examination was assessed for all the patients, and chronic periodontitis was defined through clinical attachment level and probing pocket depth, according to the American Association of Periodontology. Nonsurgical periodontal treatment was performed and serum levels of CRP were evaluated at baseline and 8 weeks after periodontal treatment. Periodontal treatment resulted in significant reductions in CRP levels (p periodontitis. Periodontitis is an important source of systemic inflammation in CKD patients. Nonsurgical periodontal treatment can effectively reduce the serum level of CRP in these patients.

  20. Maintenance of asymmetric cellular localization of an auxin transport protein through interaction with the actin cytoskeleton

    Science.gov (United States)

    Muday, G. K.

    2000-01-01

    In shoots, polar auxin transport is basipetal (that is, from the shoot apex toward the base) and is driven by the basal localization of the auxin efflux carrier complex. The focus of this article is to summarize the experiments that have examined how the asymmetric distribution of this protein complex is controlled and the significance of this polar distribution. Experimental evidence suggests that asymmetries in the auxin efflux carrier may be established through localized secretion of Golgi vesicles, whereas an attachment of a subunit of the efflux carrier to the actin cytoskeleton may maintain this localization. In addition, the idea that this localization of the efflux carrier may control both the polarity of auxin movement and more globally regulate developmental polarity is explored. Finally, evidence indicating that the gravity vector controls auxin transport polarity is summarized and possible mechanisms for the environmentally induced changes in auxin transport polarity are discussed.

  1. Pregnancy-associated plasma protein-A in patients on Maintenance Hemodialysis

    International Nuclear Information System (INIS)

    Abdel-Messeih, Ph.L.

    2012-01-01

    A high level of serum pregnancy associated plasma protein-A (PAPP-A) has been observed in patients suffering from renal impairment. The aim of the present study is to evaluate the level of PAPP-A and to elucidate its relationship with renal osteodystrophy and renal functions in patients maintained on hemodialysis (HD). Intact parathyroid hormone (i-PTH), calcium (Ca) and phosphorus (P) levels and alkaline phosphatase activity (ALP) were measured in the serum as markers of renal osteodystrophy while the level of blood urea and serum creatinine were evaluated as markers of renal functions. The results obtained showed that for patients maintained on HD, the levels of PAPP-A, i-PTH, P, urea and creatinine, were significantly higher than controls. Significant positive correlations were obtained between PAPP-A and each of i-PTH, ALP and creatinine in the same group. After dialysis session, the level of PAPP-A increased significantly, compared to its pre -dialysis level. According to the results obtained in the current study, it could be concluded that the increase in PAPP-A level in the serum of patients maintained on hemodialysis is probably the result of chronic inflammation and impairment of kidney functions rather than renal osteodystrophy

  2. The effect of a low-fat, high-protein or high-carbohydrate ad libitum diet on weight loss maintenance and metabolic risk factors.

    Science.gov (United States)

    Claessens, M; van Baak, M A; Monsheimer, S; Saris, W H M

    2009-03-01

    High-protein (HP) diets are often advocated for weight reduction and weight loss maintenance. The aim was to compare the effect of low-fat, high-carbohydrate (HC) and low-fat, HP ad libitum diets on weight maintenance after weight loss induced by a very low-calorie diet, and on metabolic and cardiovascular risk factors in healthy obese subjects. Forty-eight subjects completed the study that consisted of an energy restriction period of 5-6 weeks followed by a weight maintenance period of 12 weeks. During weight maintenance subjects received maltodextrin (HC group) or protein (HP group) (casein (HPC subgroup) or whey (HPW subgroup)) supplements (2 x 25 g per day), respectively and consumed a low-fat diet. Subjects in the HP diet group showed significantly better weight maintenance after weight loss (2.3 kg difference, P=0.04) and fat mass reduction (2.2 kg difference, P=0.02) than subjects in the HC group. Triglyceride (0.6 mM difference, P=0.01) and glucagon (9.6 pg ml(-1) difference, P=0.02) concentrations increased more in the HC diet group, while glucose (0.3 mM difference, P=0.02) concentration increased more in the HP diet group. Changes in total cholesterol, low-density lipoprotein-cholesterol, high-density lipoprotein-cholesterol, insulin, HOMAir index, HbA1c, leptin and adiponectin concentrations did not differ between the diets. No differences were found between the casein- or whey-supplemented HP groups. These results show that low-fat, high-casein or whey protein weight maintenance diets are more effective for weight control than low-fat, HC diets and do not adversely affect metabolic and cardiovascular risk factors in weight-reduced moderately obese subjects without metabolic or cardiovascular complications.

  3. Post-irradiation phosphorylation of structural maintenance chromosome 1 (SMC1) is independent of the Fanconi protein pathway

    International Nuclear Information System (INIS)

    Nahas, Shareef A.; Lai, C.-H.; Gatti, Richard A.

    2005-01-01

    Purpose: To confirm the sensitivity of cells from patients with Fanconi anemia (FA) to ionizing radiation, and to determine whether the phosphorylation of structural maintenance chromosome 1 (SMC1) was associated with radiosensitivity, as it is in other DNA repair disorders. Methods and materials: Using lymphoblastoid cell lines from FA patients before and after exposure to ionizing radiation, the colony survival assay, radioresistant DNA synthesis, and SMC1 phosphorylation were measured. FA lymphoblastoid cell lines that had been transfected with the wild-type FANC gene were used as controls. Results: Cells from FA patients of six complementation groups were radiosensitive. Despite this, SMC1 phosphorylation was normal in each case; radioresistant DNA synthesis, a measure of S phase checkpoint integrity, was defective in FANCD2 lymphoblastoid cell lines and was corrected in FANCD2 + D2 cells. Conclusions: The data indicate that the FANC pathway proteins play a major role in the cellular responses to ionizing radiation, but not in SMC1 phosphorylation or in the S phase checkpoint of FANCD2-deficient cells. Thus, SMC1 activation is not a common denominator of radiosensitivity, as has been suggested by radiation responses of cells from ataxia-telangiectasia, Nijmegen breakage syndrome, or Mre11 deficiency patients

  4. Weight loss maintenance in overweight subjects on ad libitum diets with high or low protein content and glycemic index

    DEFF Research Database (Denmark)

    Aller, E E J G; Larsen, Thomas Meinert; Holst, Claus

    2014-01-01

    Background:A high dietary protein (P) content and low glycemic index (GI) have been suggested to be beneficial for weight management, but long-term studies are scarce.Objective:The DIOGENES randomized clinical trial investigated the effect of P and GI on weight loss maintenance in overweight...... or obese adults in 8 centers across Europe. This study reports the 1-year results in 2 of the centers that extended the intervention to 1 year.Method:After an 8-week low calorie diet (LCD), 256 adults (BMI>27 kg/m(2)) were randomized to 5 ad libitum diets for 12 months: high P/low GI (HP/LGI), high P/high...... GI (HP/HGI), low P/low GI (LP/LGI), low P/high GI (LP/HGI) and a control diet. During the first 6 months foods were provided for free through a shop system, during the whole 12-month period subjects received guidance by a dietician. Primary outcome variable was the change in body weight over the 12...

  5. Cdc45 (cell division cycle protein 45) guards the gate of the Eukaryote Replisome helicase stabilizing leading strand engagement

    Science.gov (United States)

    Petojevic, Tatjana; Pesavento, James J.; Costa, Alessandro; Liang, Jingdan; Wang, Zhijun; Berger, James M.; Botchan, Michael R.

    2015-01-01

    DNA replication licensing is now understood to be the pathway that leads to the assembly of double hexamers of minichromosome maintenance (Mcm2–7) at origin sites. Cell division control protein 45 (Cdc45) and GINS proteins activate the latent Mcm2–7 helicase by inducing allosteric changes through binding, forming a Cdc45/Mcm2-7/GINS (CMG) complex that is competent to unwind duplex DNA. The CMG has an active gate between subunits Mcm2 and Mcm5 that opens and closes in response to nucleotide binding. The consequences of inappropriate Mcm2/5 gate actuation and the role of a side channel formed between GINS/Cdc45 and the outer edge of the Mcm2–7 ring for unwinding have remained unexplored. Here we uncover a novel function for Cdc45. Cross-linking studies trace the path of the DNA with the CMG complex at a fork junction between duplex and single strands with the bound CMG in an open or closed gate conformation. In the closed state, the lagging strand does not pass through the side channel, but in the open state, the leading strand surprisingly interacts with Cdc45. Mutations in the recombination protein J fold of Cdc45 that ablate this interaction diminish helicase activity. These data indicate that Cdc45 serves as a shield to guard against occasional slippage of the leading strand from the core channel. PMID:25561522

  6. The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair.

    Directory of Open Access Journals (Sweden)

    Naoki Takahashi

    2010-01-01

    Full Text Available The DNA replication process represents a source of DNA stress that causes potentially spontaneous genome damage. This effect might be strengthened by mutations in crucial replication factors, requiring the activation of DNA damage checkpoints to enable DNA repair before anaphase onset. Here, we demonstrate that depletion of the evolutionarily conserved minichromosome maintenance helicase-binding protein ETG1 of Arabidopsis thaliana resulted in a stringent late G2 cell cycle arrest. This arrest correlated with a partial loss of sister chromatid cohesion. The lack-of-cohesion phenotype was intensified in plants without functional CTF18, a replication fork factor needed for cohesion establishment. The synergistic effect of the etg1 and ctf18 mutants on sister chromatid cohesion strengthened the impact on plant growth of the replication stress caused by ETG1 deficiency because of inefficient DNA repair. We conclude that the ETG1 replication factor is required for efficient cohesion and that cohesion establishment is essential for proper development of plants suffering from endogenous DNA stress. Cohesion defects observed upon knockdown of its human counterpart suggest an equally important developmental role for the orthologous mammalian ETG1 protein.

  7. Protein-pacing caloric-restriction enhances body composition similarly in obese men and women during weight loss and sustains efficacy during long-term weight maintenance

    DEFF Research Database (Denmark)

    Arciero, Paul J; Edmonds, Rohan; He, Feng

    2016-01-01

    Short-Term protein-pacing (P; ~6 meals/day, >30% protein/day) and caloric restriction (CR, ~25% energy deficit) improves total (TBF), abdominal (ABF) and visceral (VAT) fat loss, energy expenditure, and biomarkers compared to heart healthy (HH) recommendations (3 meals/day, 15% protein/day) in ob......Short-Term protein-pacing (P; ~6 meals/day, >30% protein/day) and caloric restriction (CR, ~25% energy deficit) improves total (TBF), abdominal (ABF) and visceral (VAT) fat loss, energy expenditure, and biomarkers compared to heart healthy (HH) recommendations (3 meals/day, 15% protein....../day) in obese adults. Less is known whether obese men and women respond similarly to P-CR during weight loss (WL) and whether a modified P-CR (mP-CR) is more efficacious than a HH diet during long-term (52 week) weight maintenance (WM). The purposes of this study were to evaluate the efficacy of: (1) P......-CR on TBF, ABF, resting metabolic rate (RMR), and biomarkers between obese men and women during WL (weeks 0-12); and (2) mP-CR compared to a HH diet during WM (weeks 13-64). During WL, men (n = 21) and women (n = 19) were assessed for TBF, ABF, VAT, RMR, and biomarkers at weeks 0 (pre) and 12 (post). Men...

  8. Role of protein synthesis and DNA methylation in the consolidation and maintenance of long-term memory in Aplysia

    OpenAIRE

    Pearce, Kaycey; Cai, Diancai; Roberts, Adam C; Glanzman, David L

    2017-01-01

    eLife digest The formation of long-term memory depends on new proteins being made in the brain. These new proteins are used partly to build the new connections among neurons that essentially store the memory, and must be made within a critical period of time. Experiments on animals have found that new proteins must be made during or shortly after training to form a stable memory; if protein synthesis is blocked during this period, the memory will not be stabilized (a process also known as mem...

  9. Maintenance methods

    International Nuclear Information System (INIS)

    Sanchis, H.; Aucher, P.

    1990-01-01

    The maintenance method applied at the Hague is summarized. The method was developed in order to solve problems relating to: the different specialist fields, the need for homogeneity in the maintenance work, the equipment diversity, the increase of the materials used at the Hague's new facilities. The aim of the method is to create a knowhow formalism, to facilitate maintenance, to ensure the running of the operations and to improve the estimation of the maintenance cost. One of the method's difficulties is the demonstration of the profitability of the maintenance operations [fr

  10. Maintenance of energy expenditure on high-protein vs. high-carbohydrate diets at a constant body weight may prevent a positive energy balance.

    Science.gov (United States)

    Martens, E A; Gonnissen, H K; Gatta-Cherifi, B; Janssens, P L; Westerterp-Plantenga, M S

    2015-10-01

    Relatively high-protein diets are effective for body weight loss, and subsequent weight maintenance, yet it remains to be shown whether these diets would prevent a positive energy balance. Therefore, high-protein diet studies at a constant body weight are necessary. The objective was to determine fullness, energy expenditure, and macronutrient balances on a high-protein low-carbohydrate (HPLC) diet compared with a high-carbohydrate low-protein (HCLP) diet at a constant body weight, and to assess whether effects are transient or sustained after 12 weeks. A randomized parallel study was performed in 14 men and 18 women [mean ± SD age: 24 ± 5 y; BMI (in kg/m(2)): 22.8 ± 2.0] on diets containing 30/35/35 (HPLC) or 5/60/35 (HCLP) % of energy from protein/carbohydrate/fat. Significant interactions between dietary intervention and time on total energy expenditure (TEE) (P = 0.013), sleeping metabolic rate (SMR) (P = 0.040), and diet-induced thermogenesis (DIT) (P = 0.027) appeared from baseline to wk 12. TEE was maintained in the HPLC diet group, while it significantly decreased throughout the intervention period in the HCLP diet group (wk 1: P = 0.002; wk 12: P = 0.001). Energy balance was maintained in the HPLC diet group, and became positive in the HCLP diet group at wk 12 (P = 0.008). Protein balance varied directly according to the amount of protein in the diet, and diverged significantly between the diets (P = 0.001). Fullness ratings were significantly higher in the HPLC vs. the HCLP diet group at wk 1 (P = 0.034), but not at wk 12. Maintenance of energy expenditure on HPLC vs. HCLP diets at a constant body weight may prevent development of a positive energy balance, despite transiently higher fullness. The study was registered on clinicaltrials.gov with Identifier: NCT01551238. Copyright © 2014 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

  11. Novel roles of folic acid as redox regulator: Modulation of reactive oxygen species sinker protein expression and maintenance of mitochondrial redox homeostasis on hepatocellular carcinoma.

    Science.gov (United States)

    Lai, Kun-Goung; Chen, Chi-Fen; Ho, Chun-Te; Liu, Jun-Jen; Liu, Tsan-Zon; Chern, Chi-Liang

    2017-06-01

    We provide herein several lines of evidence to substantiate that folic acid (or folate) is a micronutrient capable of functioning as a novel redox regulator on hepatocellular carcinoma. First, we uncovered that folate deficiency could profoundly downregulate two prominent anti-apoptotic effectors including survivin and glucose-regulated protein-78. Silencing of either survivin or glucose-regulated protein-78 via small interfering RNA interfering technique established that both effectors could serve as reactive oxygen species sinker proteins. Second, folate deficiency-triggered oxidative-nitrosative stress could strongly induce endoplasmic reticulum stress that in turn could provoke cellular glutathione depletion through the modulation of the following two crucial events: (1) folate deficiency could strongly inhibit Bcl-2 expression leading to severe suppression of the mitochondrial glutathione pool and (2) folate deficiency could also profoundly inhibit two key enzymes that governing cellular glutathione redox regulation including γ-glutamylcysteinyl synthetase heavy chain, a catalytic enzyme for glutathione biosynthesis, and mitochondrial isocitrate dehydrogenase 2, an enzyme responsible for providing nicotinamide adenine dinucleotide phosphate necessary for regenerating oxidized glutathione disulfide back to glutathione via mitochondrial glutathione reductase. Collectively, we add to the literature new data to strengthen the notion that folate is an essential micronutrient that confers a novel role to combat reactive oxygen species insults and thus serves as a redox regulator via upregulating reactive oxygen species sinker proteins and averting mitochondrial glutathione depletion through proper maintenance of redox homeostasis via positively regulating glutathione biosynthesis, glutathione transporting system, and mitochondrial glutathione recycling process.

  12. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.

    Directory of Open Access Journals (Sweden)

    Audrey Costes

    2010-12-01

    Full Text Available We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome.

  13. Intradialytic parenteral nutrition in maintenance hemodialysis patients suffering from protein-energy wasting. Results of a multicenter, open, prospective, randomized trial.

    Science.gov (United States)

    Marsen, Tobias A; Beer, Justinus; Mann, Helmut

    2017-02-01

    Protein-energy wasting (PEW) is increasingly becoming a clinical problem in maintenance hemodialysis patients and guidelines call for nutritional interventions. Serum prealbumin (transthyretin) represents a critical nutritional marker positively correlated with patient survival and negatively correlated with morbidity. Nutritional counseling, oral supplementation as well as intradialytic parenteral nutrition (IDPN) are recommended to fight PEW, however clinical trials on their use are scarce. We conducted a prospective, multicenter, randomized, open-label, controlled, parallel-group Phase IV clinical trial in 107 maintenance hemodialysis patients suffering from PEW to assess the impact of IDPN on prealbumin and other biochemical and clinical parameters reflecting nutritional status. Patients randomized to the intervention group received standardized nutritional counseling plus IDPN three times weekly over 16 weeks followed by a treatment-free period of 12 weeks. The control group received standardized nutritional counseling only. Main trial inclusion criteria included moderate to severe malnutrition (SGA score B or C), maintenance hemodialysis therapy (3 times per week) for more than six months, and presence of two out of the following three criteria: albumin 30 mg/L at week 16 (48.7% vs. 31.8%). Prealbumin response to IDPN therapy was more prominent in patients suffering from moderate malnutrition (SGA score B) compared to patients with severe malnutrition (SGA score C). The results of this trial demonstrate for the first time that IDPN therapy, given three times weekly in a 16-week short-term intervention, results in a statistically significant and clinically relevant increase in mean serum prealbumin, a surrogate marker for outcome and survival in hemodialysis patients suffering from PEW, and is superior to nutritional counseling. Clinical trial registry:www.clinicaltrials.gov (NCT00501956). Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights

  14. Associations between dairy protein intake and body weight and risk markers of diabetes and CVD during weight maintenance

    DEFF Research Database (Denmark)

    Bendtsen, Line Quist; Lorenzen, Janne Kunchel; Larsen, Thomas Meinert

    2014-01-01

    Dairy products have previously been reported to be associated with beneficial effects on body weight and metabolic risk markers. Moreover, primary data from the Diet, Obesity and Genes (DiOGenes) study indicate a weight-maintaining effect of a high-protein-low-glycaemic index diet. The objective ...

  15. Preventative Maintenance.

    Science.gov (United States)

    Migliorino, James

    Boards of education must be convinced that spending money up front for preventive maintenance will, in the long run, save districts' tax dollars. A good program of preventive maintenance can minimize disruption of service; reduce repair costs, energy consumption, and overtime; improve labor productivity and system equipment reliability; handle…

  16. Normal protein intake is required for body weight loss and weight maintenance, and elevated protein intake for additional preservation of resting energy expenditure and fat free mass.

    Science.gov (United States)

    Soenen, Stijn; Martens, Eveline A P; Hochstenbach-Waelen, Ananda; Lemmens, Sofie G T; Westerterp-Plantenga, Margriet S

    2013-05-01

    Energy-restricted high-protein diets (HPDs) have shown favorable results for body weight (BW) management, yet studies differ in their outcomes depending on the dietary protein content. Our objective was to determine the effects of dietary protein content on BW loss-related variables during a 6-mo energy restriction with the use of diets containing protein at the level of requirement [normal-protein diet (NPD), 0.8 g · kg BW(-1) (.) d(-1)] and above (HPD, 1.2 g · kg BW(-1) (.) d(-1)). In overweight and obese participants (24 men and 48 women), BW, body composition, and metabolic responses were assessed before and after subsequent energy intakes of 100, 33, and 67% of the original individual daily energy requirements. Protein intake was consistent in the NPD (0.8 ± 0.3 g · kg BW(-1) (.) d(-1)) and HPD (1.2 ± 0.3 g · kg BW(-1) (.) d(-1)) groups throughout the study (P body fat mass similarly decreased in the NPD and HPD groups (P initial sparing effect of FFM and lowering of DBP.

  17. Maintenance of the marginal-zone B cell compartment specifically requires the RNA-binding protein ZFP36L1.

    Science.gov (United States)

    Newman, Rebecca; Ahlfors, Helena; Saveliev, Alexander; Galloway, Alison; Hodson, Daniel J; Williams, Robert; Besra, Gurdyal S; Cook, Charlotte N; Cunningham, Adam F; Bell, Sarah E; Turner, Martin

    2017-06-01

    RNA-binding proteins of the ZFP36 family are best known for inhibiting the expression of cytokines through binding to AU-rich elements in the 3' untranslated region and promoting mRNA decay. Here we identified an indispensable role for ZFP36L1 as the regulator of a post-transcriptional hub that determined the identity of marginal-zone B cells by promoting their proper localization and survival. ZFP36L1 controlled a gene-expression program related to signaling, cell adhesion and locomotion; it achieved this in part by limiting expression of the transcription factors KLF2 and IRF8, which are known to enforce the follicular B cell phenotype. These mechanisms emphasize the importance of integrating transcriptional and post-transcriptional processes by RNA-binding proteins for maintaining cellular identity among closely related cell types.

  18. Maintenance of muscle myosin levels in adult C. elegans requires both the double bromodomain protein BET-1 and sumoylation

    Directory of Open Access Journals (Sweden)

    Kate Fisher

    2013-10-01

    Attenuation of RAS-mediated signalling is a conserved process essential to control cell proliferation, differentiation, and apoptosis. Cooperative interactions between histone modifications such as acetylation, methylation and sumoylation are crucial for proper attenuation in C. elegans, implying that the proteins recognising these histone modifications could also play an important role in attenuation of RAS-mediated signalling. We sought to systematically identify these proteins and found BET-1. BET-1 is a conserved double bromodomain protein that recognises acetyl-lysines on histone tails and maintains the stable fate of various lineages. Unexpectedly, adults lacking both BET-1 and SUMO-1 are depleted of muscle myosin, an essential component of myofibrils. We also show that this muscle myosin depletion does not occur in all animals at a specific time, but rather that the penetrance of the phenotype increases with age. To gain mechanistic insights into this process, we sought to delay the occurrence of the muscle myosin depletion phenotype and found that it requires caspase activity and MEK-dependent signalling. We also performed transcription profiling on these mutants and found an up-regulation of the FGF receptor, egl-15, a tyrosine kinase receptor acting upstream of MEK. Consistent with a MEK requirement, we could delay the muscle phenotype by systemic or hypodermal knock down of egl-15. Thus, this work uncovered a caspase- and MEK-dependent mechanism that acts specifically on ageing adults to maintain the appropriate net level of muscle myosin.

  19. Phosphorylation state of a Tob/BTG protein, FOG-3, regulates initiation and maintenance of the Caenorhabditis elegans sperm fate program.

    Science.gov (United States)

    Lee, Myon-Hee; Kim, Kyung Won; Morgan, Clinton T; Morgan, Dyan E; Kimble, Judith

    2011-05-31

    FOG-3, the single Caenorhabditis elegans Tob/BTG protein, directs germ cells to adopt the sperm fate at the expense of oogenesis. Importantly, FOG-3 activity must be maintained for the continued production of sperm that is typical of the male sex. Vertebrate Tob proteins have antiproliferative activity and ERK phosphorylation of Tob proteins has been proposed to abrogate "antiproliferative" activity. Here we investigate FOG-3 phosphorylation and its effect on sperm fate specification. We found both phosphorylated and unphosphorylated forms of FOG-3 in nematodes. We then interrogated the role of FOG-3 phosphorylation in sperm fate specification. Specifically, we assayed FOG-3 transgenes for rescue of a fog-3 null mutant. Wild-type FOG-3 rescued both initiation and maintenance of sperm fate specification. A FOG-3 mutant with its four consensus ERK phosphorylation sites substituted to alanines, called FOG-3(4A), rescued partially: sperm were made transiently but not continuously in both sexes. A different FOG-3 mutant with its sites substituted to glutamates, called FOG-3(4E), had no rescuing activity on its own, but together with FOG-3(4A) rescue was complete. Thus, when FOG-3(4A) and FOG-3(4E) were both introduced into the same animals, sperm fate specification was not only initiated but also maintained, resulting in continuous spermatogenesis in males. Our findings suggest that unphosphorylated FOG-3 initiates the sperm fate program and that phosphorylated FOG-3 maintains that program for continued sperm production typical of males. We discuss implications of our results for Tob/BTG proteins in vertebrates.

  20. Maintenance Mentor

    National Research Council Canada - National Science Library

    Jacobs, John

    2003-01-01

    Maintenance Mentor (MXM) is a research effort conducted by a joint AFRL/HESR and Northrop Grumman Information Technology team to identify the basic, high-level requirements necessary for improving flight line diagnostic capabilities...

  1. Instrumentation maintenance

    International Nuclear Information System (INIS)

    Mack, D.A.

    1976-09-01

    It is essential to any research activity that accurate and efficient measurements be made for the experimental parameters under consideration for each individual experiment or test. Satisfactory measurements in turn depend upon having the necessary instruments and the capability of ensuring that they are performing within their intended specifications. This latter requirement can only be achieved by providing an adequate maintenance facility, staffed with personnel competent to understand the problems associated with instrument adjustment and repair. The Instrument Repair Shop at the Lawrence Berkeley Laboratory is designed to achieve this end. The organization, staffing and operation of this system is discussed. Maintenance policy should be based on studies of (1) preventive vs. catastrophic maintenance, (2) records indicating when equipment should be replaced rather than repaired and (3) priorities established to indicate the order in which equipment should be repaired. Upon establishing a workable maintenance policy, the staff should be instructed so that they may provide appropriate scheduled preventive maintenance, calibration and corrective procedures, and emergency repairs. The education, training and experience of the maintenance staff is discussed along with the organization for an efficient operation. The layout of the various repair shops is described in the light of laboratory space and financial constraints

  2. Maintenance of the marginal zone B cell compartment specifically requires the RNA-binding protein ZFP36L1

    Science.gov (United States)

    Newman, Rebecca; Ahlfors, Helena; Saveliev, Alexander; Galloway, Alison; Hodson, Daniel J; Williams, Robert; Besra, Gurdyal S.; Cook, Charlotte N; Cunningham, Adam F; Bell, Sarah E; Turner, Martin

    2017-01-01

    RNA binding proteins (RBP) of the ZFP36 family are best known for inhibiting the expression of cytokines through binding to AU rich elements in the 3’UTR and promoting mRNA decay. Here we show an indispensible role for ZFP36L1 as the regulator of a post-transcriptional hub that determined the identity of marginal zone (MZ) B cells by promoting their proper localization and survival. ZFP36L1 controlled a gene expression program related to signaling, cell-adhesion and locomotion, in part by limiting the expression of the transcription factors KLF2 and IRF8, which are known to enforce the follicular B cell phenotype. These mechanisms emphasize the importance of integrating transcriptional and post-transcriptional processes by RBP for maintaining cellular identity between closely related cell types. PMID:28394372

  3. Comparison of C-reactive protein levels with delivered dose of Kt/V in patients with end-stage renal disease on maintenance hemodialysis

    Directory of Open Access Journals (Sweden)

    Humayun Rashid

    2015-01-01

    Full Text Available The objective of our study is to compare the C-reactive protein (CRP levels with the delivered dose of dialysis in terms of Kt/V in patients undergoing maintenance hemodialysis (HD. This is a comparative, cross-sectional survey. The study was conducted at the HD unit of the Shaikh Zayed Hospital, Lahore, Pakistan. Patients who fulfilled the inclusion and exclusion criteria were enrolled in this study. The delivered dose of HD (Kt/V was assessed by an online clearance module (OCM in Fresenius machines at the end of every dialysis session and the weekly Kt/V was determined by adding all three Kt/V values. The serum CRP sample was taken after each session of HD and the mean CRP was calculated and considered elevated if it was >6 mg/dL. Both weekly Kt/V and CRP values were entered in a pre-designed proforma. Data were analyzed by using statistical software SPSS and P-value £0.05 was considered significant. Of 100 patients on maintenance HD, high serum CRP level (>6 mg/dL was found in 38 patients. When the Kt/V was compared with the CRP level, there was a negative correlation between the two parameters (r = 0.212, P = 0.032. Low Kt/V means dialysis inadequacy, which is associated with chronic inflammatory state, resulting in high CRP levels. We suggest that the quality of life of dialysis patients can be improved by offering an adequate dose of HD reflected by Kt/V ≥3.6/week.

  4. Assessing the Association between Serum Ferritin, Transferrin Saturation, and C-Reactive Protein in Northern Territory Indigenous Australian Patients with High Serum Ferritin on Maintenance Haemodialysis

    Directory of Open Access Journals (Sweden)

    Sandawana William Majoni

    2017-01-01

    Full Text Available Objective. To determine the significance of high serum ferritin observed in Indigenous Australian patients on maintenance haemodialysis in the Northern Territory, we assessed the relationship between ferritin and transferrin saturation (TSAT as measures of iron status and ferritin and C-reactive protein (CRP as markers of inflammation. Methods. We performed a retrospective cohort analysis of data from adult patients (≥18 years on maintenance haemodialysis (>3 months from 2004 to 2011. Results. There were 1568 patients. The mean age was 53.9 (11.9 years. 1244 (79.3% were Indigenous. 44.2% (n=693 were male. Indigenous patients were younger (mean age [52.3 (11.1 versus 57.4 (15.2, p<0.001] and had higher CRP [14.7 mg/l (7–35 versus 5.9 mg/l (1.9–17.5, p<0.001], higher median serum ferritin [1069 µg/l (668–1522 versus 794.9 µg/l (558.5–1252.0, p<0.001], but similar transferrin saturation [26% (19–37 versus 28% (20–38, p=0.516]. We observed a small positive correlation between ferritin and TSAT (r2=0.11, p<0.001, no correlation between ferritin and CRP (r2 = 0.001, p<0.001, and positive association between high serum ferritin and TSAT (p<0.001, Indigenous ethnicity (p<0.001, urea reduction ratio (p=0.001, and gender (p<0.001 after adjustment in mixed regression analysis. Conclusion. Serum ferritin and TSAT may inadequately reflect iron status in this population. The high ferritin was poorly explained by inflammation.

  5. Protein-Pacing Caloric-Restriction Enhances Body Composition Similarly in Obese Men and Women during Weight Loss and Sustains Efficacy during Long-Term Weight Maintenance.

    Science.gov (United States)

    Arciero, Paul J; Edmonds, Rohan; He, Feng; Ward, Emery; Gumpricht, Eric; Mohr, Alex; Ormsbee, Michael J; Astrup, Arne

    2016-07-30

    Short-Term protein-pacing (P; ~6 meals/day, >30% protein/day) and caloric restriction (CR, ~25% energy deficit) improves total (TBF), abdominal (ABF) and visceral (VAT) fat loss, energy expenditure, and biomarkers compared to heart healthy (HH) recommendations (3 meals/day, 15% protein/day) in obese adults. Less is known whether obese men and women respond similarly to P-CR during weight loss (WL) and whether a modified P-CR (mP-CR) is more efficacious than a HH diet during long-term (52 week) weight maintenance (WM). The purposes of this study were to evaluate the efficacy of: (1) P-CR on TBF, ABF, resting metabolic rate (RMR), and biomarkers between obese men and women during WL (weeks 0-12); and (2) mP-CR compared to a HH diet during WM (weeks 13-64). During WL, men (n = 21) and women (n = 19) were assessed for TBF, ABF, VAT, RMR, and biomarkers at weeks 0 (pre) and 12 (post). Men and women had similar reductions (p 50%) and increase in % lean body mass (9%). RMR (kcals/kg bodyweight) was unchanged and respiratory quotient decreased 9%. Twenty-four subjects (mP-CR, n = 10; HH, n = 14) completed WM. mP-CR regained significantly less body weight (6%), TBF (12%), and ABF (17%) compared to HH (p < 0.05). Our results demonstrate P-CR enhances weight loss, body composition and biomarkers, and maintains these changes for 52-weeks compared to a traditional HH diet.

  6. Synapse formation and maintenance by C1q family proteins: a new class of secreted synapse organizers.

    Science.gov (United States)

    Yuzaki, Michisuke

    2010-07-01

    Several C1q family members, especially the Cbln and C1q-like subfamilies, are highly and predominantly expressed in the central nervous system. Cbln1, a member of the Cbln subfamily, plays two unique roles at parallel fiber (PF)-Purkinje cell synapses in the cerebellum: the formation and stabilization of synaptic contact, and the control of functional synaptic plasticity by regulating the postsynaptic endocytotic pathway. The delta2 glutamate receptor (GluD2), which is predominantly expressed in Purkinje cells, plays similar critical roles in the cerebellum. In addition, viral expression of GluD2 or the application of recombinant Cbln1 induces PF-Purkinje cell synaptogenesis in vitro and in vivo. Antigen-unmasking methods were necessary to reveal the immunoreactivities for endogenous Cbln1 and GluD2 at the synaptic junction of PF synapses. We propose that Cbln1 and GluD2 are located at the synaptic cleft, where various proteins undergo intricate molecular interactions with each other, and serve as a bidirectional synaptic organizer. © The Author (2010). Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd.

  7. High-sensitivity C-reactive protein is predictive of successful cardioversion for atrial fibrillation and maintenance of sinus rhythm after conversion.

    Science.gov (United States)

    Watanabe, Eiichi; Arakawa, Tomoharu; Uchiyama, Tatsushi; Kodama, Itsuo; Hishida, Hitoshi

    2006-04-14

    Cardioversion for atrial fibrillation (AF) is the most effective treatment for the restoration of sinus rhythm (SR). Recently, an elevated level of hs-CRP has been shown to be associated with AF burden, suggesting that inflammation increases the propensity for persistence of AF. We examined whether the level of high-sensitivity C-reactive protein (hs-CRP) was predictive of the outcome of cardioversion for AF. One hundred and six patients with a history of symptomatic AF lasting > or =1 day (age 63+/-14 years, mean+/-S.D.) underwent cardioversion. Echocardiography and hs-CRP assay were performed immediately prior to cardioversion. SR was restored in 84 patients (79%). By using selected cutoff values, multiple discriminant analysis revealed significant associations between successful cardioversion and a shorter duration of AF (AF duration or =60%, OR 0.92, 95% CI 0.86-0.99), and lower hs-CRP level (hs-CRP or =0.06 mg/dL, Cox proportional-hazards regression model found that only hs-CRP level was an independent predictor of AF recurrence (OR 5.30, 95% CI 2.46-11.5) after adjustment for coexisting cardiovascular risks. When patients were divided by the hs-CRP level of 0.06 mg/dL, percentage of maintenance of SR below and above the cutoff was 53% and 4%, respectively (log-rank test, pmaintenance of SR after conversion.

  8. Protein supplements after weight loss do not improve weight maintenance compared with recommended dietary protein intake despite beneficial effects on appetite sensation and energy expenditure: a randomized, controlled, double-blinded trial.

    Science.gov (United States)

    Kjølbæk, Louise; Sørensen, Lone Brinkmann; Søndertoft, Nadja Buus; Rasmussen, Carrie Klestrup; Lorenzen, Janne Kunchel; Serena, Anja; Astrup, Arne; Larsen, Lesli Hingstrup

    2017-08-01

    Background : High-protein diets increase weight loss (WL) during energy restriction; therefore, it has been suggested that additional protein intake may improve weight maintenance (WM) after WL. Objective: We investigated the effect of protein supplements from either whey with or without calcium or soy on WM success after WL compared with that of a control. Design: In a randomized, controlled, double-blinded trial, 220 participants aged 18-60 y with body mass index (in kg/m 2 ) from 27.6 to 40.4 were included. The study was initiated with an 8-wk WL period followed by a 24-wk WM period. During WM, participants consumed the following isocaloric supplements (45-48 g/d): whey and calcium (whey+), whey, soy, or maltodextrin (control). Data were collected at baseline, before WM, and after WM (weeks 0, 8, and 32, respectively) and included body composition, blood biochemistry, and blood pressure. Meal tests were performed to investigate diet-induced-thermogenesis (DIT) and appetite sensation. Compliance was tested by 24-h urinary nitrogen excretion. Results: A total of 151 participants completed the WM period. The control and 3 protein supplements did not result in different mean ± SD weight regains (whey+: 2.19 ± 4.6 kg; whey: 2.01 ± 4.6 kg; soy: 1.76 ± 4.7 kg; and control: 2.23 ± 3.8 kg; P = 0.96), fat mass regains (whey+: 0.46 ± 4.5 kg; whey: 0.11 ± 4.1 kg; soy: 0.15 ± 4.1 kg; and control: 0.54 ± 3.3 kg; P = 0.96), or improvements in lean body mass (whey+: 1.87 ± 1.7 kg; whey: 1.94 ± 1.3 kg; soy: 1.58 ± 1.4 kg; and control: 1.74 ± 1.4 kg; P = 0.50) during WM. Changes in blood pressure and blood biochemistry were not different between groups. Compared with the control, protein supplementation resulted in higher DIT (∼30 kJ/2.5 h) and resting energy expenditure (243 kJ/d) and an anorexigenic appetite-sensation profile. Conclusion: Protein supplementation does not result in improved WM success, or blood biochemistry after WL compared with the effects of

  9. Cows are not mice: the role of cyclic AMP, phosphodiesterases, and adenosine monophosphate-activated protein kinase in the maintenance of meiotic arrest in bovine oocytes.

    Science.gov (United States)

    Bilodeau-Goeseels, Sylvie

    2011-01-01

    Meiotic maturation in mammalian oocytes is initiated during fetal development, and is then arrested at the dictyate stage - possibly for several years. Oocyte meiosis resumes in preovulatory follicles in response to the lutenizing hormone (LH) surge or spontaneously when competent oocytes are removed from follicles and cultured. The mechanisms involved in meiotic arrest and resumption in bovine oocytes are not fully understood, and several studies point to important differences between oocytes from rodent and livestock species. This paper reviews earlier and contemporary studies on the effects of cAMP-elevating agents and phosphodiesterase (PDE) enzyme inhibitors on the maintenance of meiotic arrest in bovine oocytes in vitro. Contrary to results obtained with mouse oocytes, bovine oocyte meiosis is inhibited by activators of the energy sensor adenosine monophosphate-activated protein kinase (AMPK, mammalian gene PRKA), which is activated by AMP, the degradation product of cAMP. It is not clear whether or not the effects were due to AMPK activation, and they may depend on culture conditions. Evidence suggests that other signaling pathways (for example, the cGMP/nitric oxide pathway) are involved in bovine oocyte meiotic arrest, but further studies are needed to understand the interactions between the signaling pathways that lead to maturation promoting factor (MPF) being inactive or active. An improved understanding of the mechanisms involved in the control of bovine oocyte meiosis will facilitate better control of the process in vitro, resulting in increased developmental competence and increased efficiency of in vitro embryo production procedures. Copyright © 2011 Wiley Periodicals, Inc.

  10. Opportune maintenance and predictive maintenance decision support

    OpenAIRE

    Thomas , Edouard; Levrat , Eric; Iung , Benoît; Cocheteux , Pierre

    2009-01-01

    International audience; Conventional maintenance strategies on a single component are being phased out in favour of more predictive maintenance actions. These new kinds of actions are performed in order to control the global performances of the whole industrial system. They are anticipative in nature, which allows a maintenance expert to consider non-already-planned maintenance actions. Two questions naturally emerge: when to perform a predictive maintenance action; how a maintenance expert c...

  11. Network maintenance

    CERN Multimedia

    GS Department

    2009-01-01

    A site-wide network maintenance operation has been scheduled for Saturday 28 February. Most of the network devices of the general purpose network will be upgraded to a newer software version, in order to improve our network monitoring capabilities. This will result in a series of short (2-5 minutes) random interruptions everywhere on the CERN sites throughout the day. This upgrade will not affect the Computer Centre itself, Building 613, the Technical Network and the LHC experiments, dedicated networks at the pits. For further details of this intervention, please contact Netops by phone 74927 or e-mail mailto:Netops@cern.ch. IT/CS Group

  12. Network maintenance

    CERN Multimedia

    IT Department

    2009-01-01

    A site wide network maintenance has been scheduled for Saturday 28 February. Most of the network devices of the General Purpose network will be upgraded to a newer software version, in order to improve our network monitoring capabilities. This will result in a series of short (2-5 minutes) random interruptions everywhere on the CERN sites along this day. This upgrade will not affect: the Computer centre itself, building 613, the Technical Network and the LHC experiments dedicated networks at the pits. Should you need more details on this intervention, please contact Netops by phone 74927 or email mailto:Netops@cern.ch. IT/CS Group

  13. A mitogen-activated protein kinase Tmk3 participates in high osmolarity resistance, cell wall integrity maintenance and cellulase production regulation in Trichoderma reesei.

    Directory of Open Access Journals (Sweden)

    Mingyu Wang

    Full Text Available The mitogen-activated protein kinase (MAPK pathways are important signal transduction pathways conserved in essentially all eukaryotes, but haven't been subjected to functional studies in the most important cellulase-producing filamentous fungus Trichoderma reesei. Previous reports suggested the presence of three MAPKs in T. reesei: Tmk1, Tmk2, and Tmk3. By exploring the phenotypic features of T. reesei Δtmk3, we first showed elevated NaCl sensitivity and repressed transcription of genes involved in glycerol/trehalose biosynthesis under higher osmolarity, suggesting Tmk3 participates in high osmolarity resistance via derepression of genes involved in osmotic stabilizer biosynthesis. We also showed significant downregulation of genes encoding chitin synthases and a β-1,3-glucan synthase, decreased chitin content, 'budded' hyphal appearance typical to cell wall defective strains, and increased sensitivity to calcofluor white/Congo red in the tmk3 deficient strain, suggesting Tmk3 is involved in cell wall integrity maintenance in T. reesei. We further observed the decrease of cellulase transcription and production in T. reesei Δtmk3 during submerged cultivation, as well as the presence of MAPK phosphorylation sites on known transcription factors involved in cellulase regulation, suggesting Tmk3 is also involved in the regulation of cellulase production. Finally, the expression of cell wall integrity related genes, the expression of cellulase coding genes, cellulase production and biomass accumulation were compared between T. reesei Δtmk3 grown in solid state media and submerged media, showing a strong restoration effect in solid state media from defects resulted from tmk3 deletion. These results showed novel physiological processes that fungal Hog1-type MAPKs are involved in, and present the first experimental investigation of MAPK signaling pathways in T. reesei. Our observations on the restoration effect during solid state cultivation suggest

  14. A Mitogen-Activated Protein Kinase Tmk3 Participates in High Osmolarity Resistance, Cell Wall Integrity Maintenance and Cellulase Production Regulation in Trichoderma reesei

    Science.gov (United States)

    Wang, Mingyu; Zhao, Qiushuang; Yang, Jinghua; Jiang, Baojie; Wang, Fangzhong; Liu, Kuimei; Fang, Xu

    2013-01-01

    The mitogen-activated protein kinase (MAPK) pathways are important signal transduction pathways conserved in essentially all eukaryotes, but haven't been subjected to functional studies in the most important cellulase-producing filamentous fungus Trichoderma reesei. Previous reports suggested the presence of three MAPKs in T. reesei: Tmk1, Tmk2, and Tmk3. By exploring the phenotypic features of T. reesei Δtmk3, we first showed elevated NaCl sensitivity and repressed transcription of genes involved in glycerol/trehalose biosynthesis under higher osmolarity, suggesting Tmk3 participates in high osmolarity resistance via derepression of genes involved in osmotic stabilizer biosynthesis. We also showed significant downregulation of genes encoding chitin synthases and a β-1,3-glucan synthase, decreased chitin content, ‘budded’ hyphal appearance typical to cell wall defective strains, and increased sensitivity to calcofluor white/Congo red in the tmk3 deficient strain, suggesting Tmk3 is involved in cell wall integrity maintenance in T. reesei. We further observed the decrease of cellulase transcription and production in T. reesei Δtmk3 during submerged cultivation, as well as the presence of MAPK phosphorylation sites on known transcription factors involved in cellulase regulation, suggesting Tmk3 is also involved in the regulation of cellulase production. Finally, the expression of cell wall integrity related genes, the expression of cellulase coding genes, cellulase production and biomass accumulation were compared between T. reesei Δtmk3 grown in solid state media and submerged media, showing a strong restoration effect in solid state media from defects resulted from tmk3 deletion. These results showed novel physiological processes that fungal Hog1-type MAPKs are involved in, and present the first experimental investigation of MAPK signaling pathways in T. reesei. Our observations on the restoration effect during solid state cultivation suggest that T. reesei

  15. Optimizing preventive maintenance with maintenance templates

    International Nuclear Information System (INIS)

    Dozier, I.J.

    1996-01-01

    Rising operating costs has caused maintenance professionals to rethink their strategy for preventive maintenance (PM) programs. Maintenance Templates are pre-engineered PM task recommendations for a component type based on application of the component. Development of the maintenance template considers the dominant failure cause of the component and the type of preventive maintenance that can predict or prevent the failure from occurring. Maintenance template development also attempts to replace fixed frequency tasks with condition monitoring tasks such as vibration analysis or thermography. For those components that have fixed frequency PM intervals, consideration is given to the maintenance drivers such as criticality, environment and usage. This helps to maximize the PM frequency intervals and maximize the component availability. Maintenance Templates have been used at PECO Energy's Limerick Generating Station during the Reliability Centered Maintenance (RCM) Process to optimize their PM program. This paper describes the development and uses of the maintenance templates

  16. Effects of Low-Fat Diets Differing in Protein and Carbohydrate Content on Cardiometabolic Risk Factors during Weight Loss and Weight Maintenance in Obese Adults with Type 2 Diabetes

    Directory of Open Access Journals (Sweden)

    Nerylee Watson

    2016-05-01

    Full Text Available Despite evidence for the benefits of higher-protein (HP diets in weight loss, their role in type 2 diabetes mellitus (T2DM management and weight maintenance is not clear. This randomised study compared the effects of a HP diet (38% carbohydrate, 30% protein, 29% fat to a isocaloric higher-carbohydrate diet (HC: 53%:21%:23% on cardiometabolic risk factors for 12 weeks in energy restriction (~30% reduction followed by 12 weeks of energy balance whilst performing regular exercise. Outcomes were measured at baseline and the end of each phase. Sixty-one overweight/obese adults (BMI (body mass index 34.3 ± 5.1 kg/m2, aged 55 ± 8 years with T2DM who commenced the study were included in the intention-to-treat analysis including the 17 participants (HP n = 9, HC n = 8 who withdrew. Following weight loss (M ± SEM: −7.8 ± 0.6 kg, there were significant reductions in HbA1c (−1.4% ± 0.1%, p < 0.001 and several cardiometabolic health risk factors. Improvements were sustained for 12 weeks when weight was stabilised and weight loss maintained. Both the HP and HC dietary patterns with concurrent exercise may be effective strategies for weight loss and weight maintenance in T2DM although further studies are needed to determine the longer term effects of weight maintenance.

  17. Effects of Low-Fat Diets Differing in Protein and Carbohydrate Content on Cardiometabolic Risk Factors during Weight Loss and Weight Maintenance in Obese Adults with Type 2 Diabetes.

    Science.gov (United States)

    Watson, Nerylee; Dyer, Kathryn; Buckley, Jonathan; Brinkworth, Grant; Coates, Alison; Parfitt, Gaynor; Howe, Peter; Noakes, Manny; Murphy, Karen

    2016-05-12

    Despite evidence for the benefits of higher-protein (HP) diets in weight loss, their role in type 2 diabetes mellitus (T2DM) management and weight maintenance is not clear. This randomised study compared the effects of a HP diet (38% carbohydrate, 30% protein, 29% fat) to a isocaloric higher-carbohydrate diet (HC: 53%:21%:23%) on cardiometabolic risk factors for 12 weeks in energy restriction (~30% reduction) followed by 12 weeks of energy balance whilst performing regular exercise. Outcomes were measured at baseline and the end of each phase. Sixty-one overweight/obese adults (BMI (body mass index) 34.3 ± 5.1 kg/m², aged 55 ± 8 years) with T2DM who commenced the study were included in the intention-to-treat analysis including the 17 participants (HP n = 9, HC n = 8) who withdrew. Following weight loss (M ± SEM: -7.8 ± 0.6 kg), there were significant reductions in HbA1c (-1.4% ± 0.1%, p loss maintained. Both the HP and HC dietary patterns with concurrent exercise may be effective strategies for weight loss and weight maintenance in T2DM although further studies are needed to determine the longer term effects of weight maintenance.

  18. The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis.

    Science.gov (United States)

    Nagata, Mariko; Ishino, Sonoko; Yamagami, Takeshi; Ogino, Hiromi; Simons, Jan-Robert; Kanai, Tamotsu; Atomi, Haruyuki; Ishino, Yoshizumi

    2017-10-13

    The archaeal minichromosome maintenance (MCM) has DNA helicase activity, which is stimulated by GINS in several archaea. In the eukaryotic replicative helicase complex, Cdc45 forms a complex with MCM and GINS, named as CMG (Cdc45-MCM-GINS). Cdc45 shares sequence similarity with bacterial RecJ. A Cdc45/RecJ-like protein from Thermococcus kodakarensis shows a bacterial RecJ-like exonuclease activity, which is stimulated by GINS in vitro. Therefore, this archaeal Cdc45/RecJ is designated as GAN, from GINS-associated nuclease. In this study, we identified the CMG-like complex in T. kodakarensis cells. The GAN·GINS complex stimulated the MCM helicase, but MCM did not affect the nuclease activity of GAN in vitro. The gene disruption analysis showed that GAN was non-essential for its viability but the Δgan mutant did not grow at 93°C. Furthermore, the Δgan mutant showed a clear retardation in growth as compared with the parent cells under optimal conditions at 85°C. These deficiencies were recovered by introducing the gan gene encoding the nuclease deficient GAN protein back to the genome. These results suggest that the replicative helicase complex without GAN may become unstable and ineffective in replication fork progression. The nuclease activity of GAN is not related to the growth defects of the Δgan mutant cells. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  19. GMI1, a structural-maintenance-of-chromosomes-hinge domain-containing protein, is involved in somatic homologous recombination in Arabidopsis

    Czech Academy of Sciences Publication Activity Database

    Bohmdorfer, G.; Schleiffer, A.; Brunmeir, R.; Ferscha, S.; Nizhynska, V.; Kozák, Jaroslav; Angelis, Karel; Kreil, D. P.; Schweizer, D.

    2011-01-01

    Roč. 67, č. 3 (2011), s. 420-433 ISSN 0960-7412 R&D Projects: GA MŠk 1M0505; GA MŠk(CZ) LC06004 Institutional research plan: CEZ:AV0Z50380511 Keywords : structural maintenance of chromosomes * DNA repair * somatic homologous recombination Subject RIV: EI - Biotechnology ; Bionics Impact factor: 6.160, year: 2011

  20. Maintenance Business Plans.

    Science.gov (United States)

    Adams, Matt

    2002-01-01

    Discusses maintenance business plans, statements which provide accountability for facilities maintenance organizations' considerable budgets. Discusses the plan's components: statement of plan objectives, macro and detailed description of the facility assets, maintenance function descriptions, description of key performance indicators, milestone…

  1. Maintenance Management Update.

    Science.gov (United States)

    Sternloff, Robert E.

    1987-01-01

    Current trends in park maintenance are overviewed, including maintenance impact statements, avoidance of cost through efficient use and national resource conservation, horticultural accomplishments that influence maintenance management, and vandalism prevention. (CB)

  2. A Situational Maintenance Model

    DEFF Research Database (Denmark)

    Luxhoj, James T.; Thorsteinsson, Uffe; Riis, Jens Ove

    1997-01-01

    An overview of trend in maintenance management and presentation of a situational model and an analytical tools for identification of managerial efforts in maintenance.......An overview of trend in maintenance management and presentation of a situational model and an analytical tools for identification of managerial efforts in maintenance....

  3. Effects of weight loss and long-term weight maintenance with diets varying in protein and glycemic index on cardiovascular risk factors

    DEFF Research Database (Denmark)

    Gögebakan, Özlem; Kohl, Angela; Osterhoff, Martin A.

    2011-01-01

    We sought to separately examine the effects of either weight loss or diets varying in protein content and glycemic index without further changes in body weight on cardiovascular risk factors within the Diet, Obesity, and Genes study (DiOGenes).......We sought to separately examine the effects of either weight loss or diets varying in protein content and glycemic index without further changes in body weight on cardiovascular risk factors within the Diet, Obesity, and Genes study (DiOGenes)....

  4. Preventive maintenance and reliability-centered maintenance

    International Nuclear Information System (INIS)

    Anderson, J.G.

    1989-01-01

    In the recent past, the primary function of the preventive maintenance program at a nuclear power plant was to maintain the operability and reliability of the plant. Reducing overall maintenance costs by performing effective preventive maintenance, instead of more costly and disruptive corrective maintenance, instead of more costly and disruptive corrective maintenance, was a secondary consideration. In today's operating environment, the preventive maintenance program must meet the test of cost-effectiveness as well as many new standards for performance that are being required by rate regulators. The preventive maintenance program must be able to withstand the challenge of independent audit to a new standard of technical adequacy and compliance with recommendations from component vendors. In this new environment, the standard that is being applied at the San Onofre nuclear generating station (SONGS) is that the preventive maintenance program must meet the test of cost-effectiveness, improve equipment performance, support increased plant availability, and (a) literally implement the recommendations of the vendor, (b) provide other compensation for the vendor recommendation, or (c) provide technical justification for the deviation. The subject of this paper is the way that reliability-centered maintenance (RCM) is helping SONGS meet these challenges

  5. Advanced maintenance research programs

    International Nuclear Information System (INIS)

    Marston, T.U.; Gelhaus, F.; Burke, R.

    1985-01-01

    The purpose of this paper is to provide the reader with an idea of the advanced maintenance research program at the Electric Power Research Institute (EPRI). A brief description of the maintenance-related activities is provided as a foundation for the advanced maintenance research projects. The projects can be divided into maintenance planning, preventive maintenance program development and implementation, predictive (or conditional) maintenance, and innovative maintenance techniques. The projects include hardware and software development, human factors considerations, and technology promotion and implementation. The advanced concepts include: the incorporation of artificial intelligence into outage planning; turbine and pump maintenance; rotating equipment monitoring and diagnostics with the aid of expert systems; and the development of mobile robots for nuclear power plant maintenance

  6. Fiscal 1997 R and D project on industrial science and technology under a consignment from NEDO. R and D of the application technology of functional protein complex (R and D of the high-functional maintenance technology for power plants); 1997 nendo sangyo kagaku gijutsu kenkyu kaihatsu jigyo Shin energy Sangyo gijutsu Sogo Kaihatsu Kiko itaku. Kinosei tanpakushitsu shugotai oyo gijutsu no kenkyu kaihatsu (hatsuden shisetsuyo kokino maintenance gijutsu kaihatsu) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    In order to develop advanced maintenance technology for power generation facilities, application technology of functional protein complexes is under investigation. On a light controllable enzymatic reaction system, construction of a light controllable bio-reactor by physico-chemical and bio- chemical techniques, and structure and functions of a photosynthetic protein complex using thermophilic cyanobacteria as material are under investigation. On a molecular mechanism for recognition and reaction of receptor proteins, structure analysis and engineering application of glutamate receptor channels of a synaptic transmission system, and basic study and material proteins for artificial structures using endocytic micromachinery, and a multi-enzyme system for synthesis of glycosphingolipids are under investigation. On a complex oxidation/reduction system by genetic engineering technique, construction of a high- efficiency stable system with functionally arranged oxidoreductase and electron transmission systems in biomembranes is in promotion. Research work was also made on molecular assemblies for a functional protein system. 166 refs., 91 figs., 9 tabs.

  7. Autophagy Proteins ATG5 and ATG7 Are Essential for the Maintenance of Human CD34+ Hematopoietic Stem-Progenitor Cells

    NARCIS (Netherlands)

    Gomez Puerto, Maria Catalina; Folkerts, Hendrik; Wierenga, Albertus T J; Schepers, Koen; Schuringa, Jan Jacob; Coffer, Paul J.; Vellenga, Edo

    2016-01-01

    Autophagy is a highly regulated catabolic process that involves sequestration and lysosomal degradation of cytosolic components such as damaged organelles and misfolded proteins. While autophagy can be considered to be a general cellular housekeeping process, it has become clear that it may also

  8. WHITE PANICLE3, a Novel Nucleus-Encoded Mitochondrial Protein, Is Essential for Proper Development and Maintenance of Chloroplasts and Mitochondria in Rice

    Directory of Open Access Journals (Sweden)

    Hongchang Li

    2018-06-01

    Full Text Available Mitochondria and chloroplasts are interacting organelles that play important roles in plant development. In addition to a small number proteins encoded by their own genomes, the majority of mitochondrial and chloroplast proteins are encoded in the cell nucleus and imported into the organelle. As a consequence, coordination between mitochondria, chloroplasts, and the nucleus is of crucial importance to plant cells. Variegated mutants are chloroplast-defective mutants and are considered to be ideal models for studying the intercommunication between these organelles. Here, we report the isolation of WHITE PANICLE3 (WP3, a nuclear gene involved in variegation, from a naturally occurring white panicle rice mutant. Disrupted expression of WP3 in the mutant leads to severe developmental defects in both chloroplasts and mitochondria, and consequently causes the appearance of white-striped leaves and white panicles in the mutant plants. Further investigation showed that WP3 encodes a protein most likely targeted to mitochondria and is specifically expressed in rice panicles. Interestingly, we demonstrate that the recessive white-panicle phenotype in the wp3 mutant is inherited in a typical Mendelian manner, while the white-striped leaf phenotype in wp3 is maternally inherited. Our data collectively suggest that the nucleus-encoded mitochondrial protein, WP3, plays an essential role in the regulation of chloroplast development in rice panicles by maintaining functional mitochondria. Therefore, the wp3 mutant is an excellent model in which to explore the communication between the nucleus, mitochondria, and chloroplasts in plant cells.

  9. Maintenance work management system

    International Nuclear Information System (INIS)

    Kanai, T.; Takahashi, Y.; Takahashi, K.; Nishino, M.; Takeshige, R.

    2000-01-01

    The maintenance work management system supports the efficient drawing up of various documents for the maintenance work at nuclear power stations and the speeding up of the permission procedure. In addition, it improves the quality assurance of the safety and reliability of the maintenance work. Key merits of the system are: 1. Efficiently drawing up various documents for the maintenance work by using the stored data for the previous maintenance work and the pipe and instrument diagram (P and ID) data. 2. Supporting the management work for the completion of maintenance work safety by using the isolation information stored on the computer system. 3. Speeding up the permission procedure by electronic mail and electronic permission. 4. Displaying additional information such as the specifications of equipment, maintenance result, and maintenance plan by linking up with the database of another system. 5. Reducing the cost of hardware devices by using client/server network configurations of personal computers and a personal computer server. (author)

  10. Unsurfaced Road Maintenance Management

    Science.gov (United States)

    1992-12-01

    This draft manual describes an unsurfaced road maintenance management system for use on military installations. This system is available in either a manual or computerized mode (Micro PAVER). The maintenance standards prescribed should protect Govern...

  11. Winter maintenance performance measure.

    Science.gov (United States)

    2016-01-01

    The Winter Performance Index is a method of quantifying winter storm events and the DOTs response to them. : It is a valuable tool for evaluating the States maintenance practices, performing post-storm analysis, training : maintenance personnel...

  12. Competence within Maintenance

    OpenAIRE

    Nerland, Annette Smørholm

    2010-01-01

    Maintenance can be a contributing factor to unwanted events, as well as desired events and states. Human competence can be defined as the ability to perform a specific task, action or function successfully, and is therefore a key factor to proper execution of maintenance tasks. Hence,maintenance will have negative consequences if done wrong, and give positive results when done right. The purpose of this report is to study the many aspects of maintenance competence. Endeavoring to improve ...

  13. Nutritional status of maintenance dialysis patients

    DEFF Research Database (Denmark)

    Koefoed, Mette Juul; Kromann, Charles Boy; Juliussen, Sophie Ryberg

    2016-01-01

    Background and Aims Maintenance dialysis patients are at increased risk of abnormal nutritional status due to numerous causative factors, both nutritional and non-nutritional. The present study assessed the current prevalence of protein-energy wasting, low lean bodymass index and obesity...... protein catabolic rate in order to assess the prevalence of protein-energy wasting, low lean body mass index and obesity in these patients. Results Seventy-nine eligible maintenance dialysis patients participated. The prevalence of proteinenergy wasted patients was 4%(95%CI: 2-12) as assessed......-energy wasting and obesity varied considerably, depending on nutritional assessment methodology. Conclusions Our data indicate that protein-energy wasting is uncommon, whereas low lean body mass index and obesity are frequent conditions among patients in maintenance dialysis. A focus on how to increase...

  14. Role of nifedipine-sensitive sympathetic vasoconstriction in maintenance of high blood pressure in spontaneously hypertensive rats: effect of Gi-protein inactivation by pertussis toxin

    Czech Academy of Sciences Publication Activity Database

    Pintérová, Mária; Karen, Petr; Kuneš, Jaroslav; Zicha, Josef

    2010-01-01

    Roč. 28, č. 5 (2010), s. 969-978 ISSN 0263-6352 R&D Projects: GA MŠk(CZ) 1M0510; GA ČR(CZ) GA305/08/0139; GA AV ČR(CZ) IAA500110902 Institutional research plan: CEZ:AV0Z50110509 Keywords : calcium channels * inhibitory G proteins * pertussis toxin Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery Impact factor: 3.980, year: 2010

  15. Noradrenergic mechanisms and high blood pressure maintenance in genetic hypertension: The role of Gi proteins and voltage-dependent calcium channels

    Czech Academy of Sciences Publication Activity Database

    Zicha, Josef; Pintérová, Mária; Líšková, Silvia; Dobešová, Zdenka; Kuneš, Jaroslav

    2007-01-01

    Roč. 29, č. 4 (2007), s. 229-229 ISSN 1064-1963. [International symposium on SHR /12./. 20.10.2006-21.10.2006, Kyoto] R&D Projects: GA MZd(CZ) NR7786 Institutional research plan: CEZ:AV0Z50110509 Keywords : genetic hypertension * noradrenergic mechanisms * Gi proteins * voltage-dependent calcium channels Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

  16. Asphalt in Pavement Maintenance.

    Science.gov (United States)

    Asphalt Inst., College Park, MD.

    Maintenance methods that can be used equally well in all regions of the country have been developed for the use of asphalt in pavement maintenance. Specific information covering methods, equipment and terminology that applies to the use of asphalt in the maintenance of all types of pavement structures, including shoulders, is provided. In many…

  17. Optimization of surface maintenance

    International Nuclear Information System (INIS)

    Oeverland, E.

    1990-01-01

    The present conference paper deals with methods of optimizing the surface maintenance of steel-made offshore installations. The paper aims at identifying important approaches to the problems regarding the long-range planning of an economical and cost effective maintenance program. The methods of optimization are based on the obtained experiences from the maintenance of installations on the Norwegian continental shelf. 3 figs

  18. Dukovany NPP maintenance management

    International Nuclear Information System (INIS)

    Siegel, F.

    2005-01-01

    Maintenance planning and management for the technological equipment of the Dukovany NPP are described. A Maintenance Control and Evaluation System has been developed and is in use to help manage the complex maintenance issue. Practical examples of outputs of the System, with a comprehensive use of the cost, reliability and safety related data, are presented. (author)

  19. New method for quality testing of food proteins for the maintenance metabolism. 1. Studies of urinary /sup 15/N excretion by /sup 15/N-labelled young rats fed with various proteins

    Energy Technology Data Exchange (ETDEWEB)

    Bergner, H; Bergner, U; Adam, K [Humboldt-Universitaet, Berlin (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin

    1978-05-01

    Over a period of 7 days 38 Wistar rats received supplements of /sup 15/N in the form of ammonium acetate added to a casein methionine diet. From the 8th day groups of 4 or 5 rats were fed different protein diets (115 kcal per kg body weight sup(0.75)) over a 5-day period. The relationship between /sup 15/N excreted via urine and the quantity of N absorbed from the food protein was used for determining the protein quality under conservation conditions. The following order of protein quality was found: fish meal, casein, wheat, whole egg, soybean, yeast, pea, and gelatin.

  20. New method for quality testing of food proteins for the maintenance metabolism. I. Studies of urinary /sup 15/N excretion by /sup 15/N-labelled young rats fed with various proteins

    Energy Technology Data Exchange (ETDEWEB)

    Bergner, H; Bergner, U; Adam, K [Humboldt-Universitaet, Berlin (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin

    1978-05-01

    Over a period of 7 days 38 Wistar rats received supplements of /sup 15/N in the form of ammonium acetate added to a casein methionine diet. From the 8th day groups of 4 or 5 rats were fed different protein diets (115 kcal per kg body weight sup(0.75)) over a 5-day period. The relationship between /sup 15/N excreted via urine and the quantity of N absorbed from the food protein was used for determining the protein quality under conservation conditions. The following order of protein quality was found: fish meal, casein, wheat, whole egg, soybean, yeast, pea, and gelatin.

  1. The RecJ2 protein in the thermophilic archaeon Thermoplasma acidophilum is a 3'-5' exonuclease that associates with a DNA replication complex.

    Science.gov (United States)

    Ogino, Hiromi; Ishino, Sonoko; Kohda, Daisuke; Ishino, Yoshizumi

    2017-05-12

    RecJ/cell division cycle 45 (Cdc45) proteins are widely conserved in the three domains of life, i.e. in bacteria, Eukarya, and Archaea. Bacterial RecJ is a 5'-3' exonuclease and functions in DNA repair pathways by using its 5'-3' exonuclease activity. Eukaryotic Cdc45 has no identified enzymatic activity but participates in the CMG complex, so named because it is composed of Cdc45, minichromosome maintenance protein complex (MCM) proteins 2-7, and GINS complex proteins (Sld5, Psf11-3). Eukaryotic Cdc45 and bacterial/archaeal RecJ share similar amino acid sequences and are considered functional counterparts. In Archaea, a RecJ homolog in Thermococcus kodakarensis was shown to associate with GINS and accelerate its nuclease activity and was, therefore, designated GAN ( G INS- a ssociated n uclease); however, to date, no archaeal RecJ·MCM·GINS complex has been isolated. The thermophilic archaeon Thermoplasma acidophilum has two RecJ-like proteins, designated TaRecJ1 and TaRecJ2. TaRecJ1 exhibited DNA-specific 5'-3' exonuclease activity, whereas TaRecJ2 had 3'-5' exonuclease activity and preferred RNA over DNA. TaRecJ2, but not TaRecJ1, formed a stable complex with TaGINS in a 2:1 molar ratio. Furthermore, the TaRecJ2·TaGINS complex stimulated activity of TaMCM ( T. acidophilum MCM) helicase in vitro , and the TaRecJ2·TaMCM·TaGINS complex was also observed in vivo However, TaRecJ2 did not interact with TaMCM directly and was not required for the helicase activation in vitro These findings suggest that the function of archaeal RecJ in DNA replication evolved divergently from Cdc45 despite conservation of the CMG-like complex formation between Archaea and Eukarya. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  2. EPR design for maintenance

    International Nuclear Information System (INIS)

    Krugmann, U.

    1998-01-01

    Preventive maintenance is very important in achieving high plant availability. For the European Pressurized Reactor (EPR) preventive maintenance has been carefully addressed in the design stage. This is particularly necessary because of the traditionally different maintenance strategies employed in France and Germany. This paper emphasizes the following features introduced in the ERP design to minimize the duration of the refueling outage: (1) containment accessibility during power operation; (2) overall plant layout to facilitate inspections and maintenances within the containment; and (3) safety system design for enabling preventive maintenance during power operation. (author)

  3. Modern electronic maintenance principles

    CERN Document Server

    Garland, DJ

    2013-01-01

    Modern Electronic Maintenance Principles reviews the principles of maintaining modern, complex electronic equipment, with emphasis on preventive and corrective maintenance. Unfamiliar subjects such as the half-split method of fault location, functional diagrams, and fault finding guides are explained. This book consists of 12 chapters and begins by stressing the need for maintenance principles and discussing the problem of complexity as well as the requirements for a maintenance technician. The next chapter deals with the connection between reliability and maintenance and defines the terms fai

  4. Managing nuclear maintenance

    International Nuclear Information System (INIS)

    Anon.

    1990-01-01

    For utilities operating nuclear powerplants, the rules of the game continue to change. Conflicting regulatory pressures and a tougher competitive environment will make management's job more complicated and difficult in the 1990s. Dealing with these pressures successfully requires greater attention to maintenance effectiveness. Utilities can help shape their future environment by developing a well-planned strategy to guide their actions. Parts of the strategy that are discussed include developing a sound maintenance philosophy, selecting a service company, radiation exposure, and managing spare parts. This article also addresses the Swedish experience in maintenance, German philosophy regarding maintenance and the current maintenance practices of the Electricite de France

  5. AIRCRAFT MAINTENANCE HANGAR

    Directory of Open Access Journals (Sweden)

    GEAMBASU Gabriel George

    2017-05-01

    Full Text Available The paper presents the maintenance process that is done on an airplane, at a certain period of time, or after a number of flight hours or cycles and describes the checks performed behind each inspection. The first part of research describes the aircraft maintenance process that has to be done after an updated maintenance manual according with aircraft type, followed by a short introduction about maintenance hangar. The second part of the paper presents a hangar design with a foldable roof and walls, which can be folded or extended, over an airplane when a maintenance process is done, or depending on weather condition.

  6. Maintenance management systems

    International Nuclear Information System (INIS)

    Rohan, M. de

    1989-01-01

    This paper is concerned principally with Maintenance Management systems and their effective introduction into organisations. Maintenance improvement is basically a problem of managing the maintenance department in the broadest sense. Improvement does not only lie in the area of special techniques, systems or procedures; although they are valuable tools, but rather in a balanced attack, carefully guided by management. Over recent years, maintenance systems have received the major emphasis and in many instances the selection of the system has become a pre-occupation, whereas the importance of each maintenance function must be recognised and good management practices applied to all maintenance activities. The ingredients for success in the implementation of maintenance management systems are summarised as: having a management committee, clear objectives, project approach using project management techniques and an enthusiastic leader, user managed and data processing supported project, realistic budget and an understanding of the financial audit requirements. (author)

  7. Knowledge based maintenance

    Energy Technology Data Exchange (ETDEWEB)

    Sturm, A [Hamburgische Electacitaets-Werke AG Hamburg (Germany)

    1998-12-31

    The establishment of maintenance strategies is of crucial significance for the reliability of a plant and the economic efficiency of maintenance measures. Knowledge about the condition of components and plants from the technical and business management point of view therefore becomes one of the fundamental questions and the key to efficient management and maintenance. A new way to determine the maintenance strategy can be called: Knowledge Based Maintenance. A simple method for determining strategies while taking the technical condition of the components of the production process into account to the greatest possible degree which can be shown. A software with an algorithm for Knowledge Based Maintenance leads the user during complex work to the determination of maintenance strategies for this complex plant components. (orig.)

  8. Knowledge based maintenance

    Energy Technology Data Exchange (ETDEWEB)

    Sturm, A. [Hamburgische Electacitaets-Werke AG Hamburg (Germany)

    1997-12-31

    The establishment of maintenance strategies is of crucial significance for the reliability of a plant and the economic efficiency of maintenance measures. Knowledge about the condition of components and plants from the technical and business management point of view therefore becomes one of the fundamental questions and the key to efficient management and maintenance. A new way to determine the maintenance strategy can be called: Knowledge Based Maintenance. A simple method for determining strategies while taking the technical condition of the components of the production process into account to the greatest possible degree which can be shown. A software with an algorithm for Knowledge Based Maintenance leads the user during complex work to the determination of maintenance strategies for this complex plant components. (orig.)

  9. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome.

    Science.gov (United States)

    Riber, Leise; Olsson, Jan A; Jensen, Rasmus B; Skovgaard, Ole; Dasgupta, Santanu; Marinus, Martin G; Løbner-Olesen, Anders

    2006-08-01

    Initiation of DNA replication in Eschericia coli requires the ATP-bound form of the DnaA protein. The conversion of DnaA-ATP to DnaA-ADP is facilitated by a complex of DnaA, Hda (homologous to DnaA), and DNA-loaded beta-clamp proteins in a process termed RIDA (regulatory inactivation of DnaA). Hda-deficient cells initiate replication at each origin mainly once per cell cycle, and the rare reinitiation events never coincide with the end of the origin sequestration period. Therefore, RIDA is not the predominant mechanism to prevent immediate reinitiation from oriC. The cellular level of Hda correlated directly with dnaA gene expression such that Hda deficiency led to reduced dnaA gene expression, and overproduction of Hda led to DnaA overproduction. Hda-deficient cells were very sensitive to variations in the cellular level of DnaA, and DnaA overproduction led to uncontrolled initiation of replication from oriC, causing severe growth retardation or cell death. Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle.

  10. Maintenance requirements for methionine and cysteine, and ...

    African Journals Online (AJOL)

    The M&C requirement for maintenance was estimated as19 mg/kg empty body weight (EBW)/d, 26 mg/kg EBW0.75/d or 87 mg/BPm0.73/d. The maintenance requirement for THR was estimated as17 mg/kg EBW/d, 22 mg/kg EBW0.75/d or 76 mg/BPm0.73/d. The concentration of M&C in 'ideal' protein for maintenance was ...

  11. Wtip- and gadd45a-interacting protein dendrin is not crucial for the development or maintenance of the glomerular filtration barrier.

    Directory of Open Access Journals (Sweden)

    Zhijie Xiao

    Full Text Available Glomerular podocyte cells are critical for the function of the renal ultrafiltration barrier. Especially, the highly specialized cell-cell junction of podocytes, the slit diaphragm, has a central role in the filtration barrier. This is highlighted by the fact that mutations in molecular components of the slit diaphragm, including nephrin and Cd2-associated protein (Cd2ap, result in proteinuric diseases in man. Dendrin is a poorly characterized cytosolic component of the slit diaphragm in where it interacts with nephrin and Cd2ap. Dendrin is highly specific for the podocyte slit diaphragm, suggesting that it has a dedicated role in the glomerular filtration barrier. In this study, we have generated a dendrin knockout mouse line and explored the molecular interactions of dendrin. Dendrin-deficient mice were viable, fertile, and had a normal life span. Morphologically, the glomerulogenesis proceeded normally and adult dendrin-deficient mice showed normal glomerular histology. No significant proteinuria was observed. Following glomerular injury, lack of dendrin did not affect the severity of the damage or the recovery process. Yeast two-hybrid screen and co-immunoprecipitation experiments showed that dendrin binds to Wt1-interacting protein (Wtip and growth arrest and DNA-damage-inducible 45 alpha (Gadd45a. Wtip and Gadd45a mediate gene transcription in the nucleus, suggesting that dendrin may have similar functions in podocytes. In line with this, we observed the relocation of dendrin to nucleus in adriamycin nephropathy model. Our results indicate that dendrin is dispensable for the function of the normal glomerular filtration barrier and that dendrin interacts with Wtip and Gadd45a.

  12. Random maintenance policies

    CERN Document Server

    Nakagawa, Toshio

    2014-01-01

    Exploring random maintenance models, this book provides an introduction to the implementation of random maintenance, and it is one of the first books to be written on this subject.  It aims to help readers learn new techniques for applying random policies to actual reliability models, and it provides new theoretical analyses of various models including classical replacement, preventive maintenance and inspection policies. These policies are applied to scheduling problems, backup policies of database systems, maintenance policies of cumulative damage models, and reliability of random redundant systems. Reliability theory is a major concern for engineers and managers, and in light of Japan’s recent earthquake, the reliability of large-scale systems has increased in importance. This also highlights the need for a new notion of maintenance and reliability theory, and how this can practically be applied to systems. Providing an essential guide for engineers and managers specializing in reliability maintenance a...

  13. Framework for Maintenance Planning

    DEFF Research Database (Denmark)

    Soares, C. Guedes; Duarte, J. Caldeira; Garbatov, Y.

    2010-01-01

    the design and during the whole life span of operational use, within an integrated framework founded on risk and reliability based techniques. The document addresses designers, decision makers and professionals responsible for or involved in establishing maintenance plans. The purpose of this document......The present document presents a framework for maintenance planning. Maintenance plays a fundamental role in counteracting degradation effects, which are present in all infrastructure and industrial products. Therefore, maintenance planning is a very critical aspect to consider both during...... is to present maintenance as an integrated approach that needs to be planned, designed, engineered, and controlled by proper qualitative and quantitative techniques. This document outlines the basic premises for maintenance planning and provides the general philosophies that can be followed and points to a best...

  14. Suncor maintenance and reliability

    Energy Technology Data Exchange (ETDEWEB)

    Little, S. [Suncor Energy, Calgary, AB (Canada)

    2006-07-01

    Fleet maintenance and reliability at Suncor Energy was discussed in this presentation, with reference to Suncor Energy's primary and support equipment fleets. This paper also discussed Suncor Energy's maintenance and reliability standard involving people, processes and technology. An organizational maturity chart that graphed organizational learning against organizational performance was illustrated. The presentation also reviewed the maintenance and reliability framework; maintenance reliability model; the process overview of the maintenance and reliability standard; a process flow chart of maintenance strategies and programs; and an asset reliability improvement process flow chart. An example of an improvement initiative was included, with reference to a shovel reliability review; a dipper trip reliability investigation; bucket related failures by type and frequency; root cause analysis of the reliability process; and additional actions taken. Last, the presentation provided a graph of the results of the improvement initiative and presented the key lessons learned. tabs., figs.

  15. Software evolution and maintenance

    CERN Document Server

    Tripathy, Priyadarshi

    2014-01-01

    Software Evolution and Maintenance: A Practitioner's Approach is an accessible textbook for students and professionals, which collates the advances in software development and provides the most current models and techniques in maintenance.Explains two maintenance standards: IEEE/EIA 1219 and ISO/IEC14764Discusses several commercial reverse and domain engineering toolkitsSlides for instructors are available onlineInformation is based on the IEEE SWEBOK (Software Engineering Body of Knowledge)

  16. Status of fusion maintenance

    International Nuclear Information System (INIS)

    Fuller, G.M.

    1984-01-01

    Effective maintenance will be an essential ingredient in determining fusion system productivity. This level of productivity will result only after close attention is paid to the entire system as an entity and appropriate integration of the elements is made. The status of fusion maintenance is reviewed in the context of the entire system. While there are many challenging developmental tasks ahead in fusion maintenance, the required technologies are available in several high-technology industries, including nuclear fission

  17. Laboratory equipment maintenance contracts.

    Science.gov (United States)

    Boudreau, D A; Scheer, W D; Catrou, P G

    1985-12-01

    The increasing level of technical sophistication and complexity found in clinical laboratory instrumentation today more than ever demands careful attention to maintenance service needs. The time-worn caution for careful definition of requirements for acquisition of a system should also carry over to acquisition of maintenance service. Guidelines are presented for specifications of terms and conditions for maintenance service from the perspective of the laboratorian in the automated clinical laboratory.

  18. Interactive videodisc in maintenance

    International Nuclear Information System (INIS)

    Zwingelstein, G.; Nguyen Van Nghi, B.

    1986-01-01

    After a recall of the videodisc characteristics, this paper presents its utilization by Electricite de France in the framework of training and maintenance. The SICMA (Interactive Communication System in Maintenance) developed and tested by Electricte de France is presented as also its utilization. It has been tested on the sites of Dampierre and Paluel in the cases of training and maintenance (deconnexion of drive rods of control elements); the conclusions of this experimentation are finally given. 4 refs [fr

  19. Turbine maintenance and modernization

    Energy Technology Data Exchange (ETDEWEB)

    Unga, E. [Teollisuuden Voima Oy, Olkiluoto (Finland)

    1998-12-31

    The disturbance-free operation of the turbine plant plays an important role in reaching good production results. In the turbine maintenance of the Olkiluoto nuclear power plant the lifetime and efficiency of turbine components and the lifetime costs are taken into account in determining the turbine maintenance and modernization/improvement program. The turbine maintenance program and improvement/modernization measures taken in the plant units are described in this presentation. (orig.)

  20. Turbine maintenance and modernization

    Energy Technology Data Exchange (ETDEWEB)

    Unga, E [Teollisuuden Voima Oy, Olkiluoto (Finland)

    1999-12-31

    The disturbance-free operation of the turbine plant plays an important role in reaching good production results. In the turbine maintenance of the Olkiluoto nuclear power plant the lifetime and efficiency of turbine components and the lifetime costs are taken into account in determining the turbine maintenance and modernization/improvement program. The turbine maintenance program and improvement/modernization measures taken in the plant units are described in this presentation. (orig.)

  1. Industrial Maintenance Strategies

    International Nuclear Information System (INIS)

    Sajjad Akbar

    2006-01-01

    Industrial plants have become more complex due to technological advancement. This has made the task of maintenance more difficult. The maintenance costs in terms of resources and downtime loss are so high that maintenance function has become a critical factor in a plant's profitability. Industry should devote as much forethought to the management of maintenance function as to production. Maintenance has grown from an art to a precise, technical engineering science. Planning, organizing scheduling and control of maintenance using modern techniques pays dividends in the form of reduced costs and increased reliability. The magnitude and the dimension of maintenance have multiplied due to development in the engineering technologies. Production cost and capacities are directly affected by the breakdown time. Total operating cost including the maintenance cost plays an important role in replacement dimension. The integrated system approach would bring forth the desired results of high maintenance standards. The standards once achieved and sustained, would add to the reliability of the plan and relieve heavy stresses and strains on the engineering logistic support. (author)

  2. Analysis of maintenance strategies

    International Nuclear Information System (INIS)

    Laakso, K.; Simola, K.

    1998-01-01

    The main topics of the presentation include: (1) an analysis model and methods to evaluate maintenance action programs and the support decision to make changes in them and (2) to understand the maintenance strategies in a systems perspective as a basis for future developments. The subproject showed how systematic models for maintenance analysis and decision support, utilising computerised and statistical tool packages, can be taken into use for evaluation and optimisation of maintenance of active systems from the safety and economic point of view

  3. Remote Maintenance Monitoring System -

    Data.gov (United States)

    Department of Transportation — The Remote Maintenance and Monitoring System (RMMS) is a collection of subsystems that includes telecommunication components, hardware, and software, which serve to...

  4. Army Maintenance System Transformation

    National Research Council Canada - National Science Library

    Gilbertson, Frank V

    2006-01-01

    .... Used in conjunction with pertinent historical data and developed with Army transformation goals in mind, General Systems thinking can provide the framework for guiding maintenance transformation...

  5. A compendium of transcription factor and Transcriptionally active protein coding gene families in cowpea (Vigna unguiculata L.).

    Science.gov (United States)

    Misra, Vikram A; Wang, Yu; Timko, Michael P

    2017-11-22

    Cowpea (Vigna unguiculata (L.) Walp.) is the most important food and forage legume in the semi-arid tropics of sub-Saharan Africa where approximately 80% of worldwide production takes place primarily on low-input, subsistence farm sites. Among the major goals of cowpea breeding and improvement programs are the rapid manipulation of agronomic traits for seed size and quality and improved resistance to abiotic and biotic stresses to enhance productivity. Knowing the suite of transcription factors (TFs) and transcriptionally active proteins (TAPs) that control various critical plant cellular processes would contribute tremendously to these improvement aims. We used a computational approach that employed three different predictive pipelines to data mine the cowpea genome and identified over 4400 genes representing 136 different TF and TAP families. We compare the information content of cowpea to two evolutionarily close species common bean (Phaseolus vulgaris), and soybean (Glycine max) to gauge the relative informational content. Our data indicate that correcting for genome size cowpea has fewer TF and TAP genes than common bean (4408 / 5291) and soybean (4408/ 11,065). Members of the GROWTH-REGULATING FACTOR (GRF) and Auxin/indole-3-acetic acid (Aux/IAA) gene families appear to be over-represented in the genome relative to common bean and soybean, whereas members of the MADS (Minichromosome maintenance deficient 1 (MCM1), AGAMOUS, DEFICIENS, and serum response factor (SRF)) and C2C2-YABBY appear to be under-represented. Analysis of the AP2-EREBP APETALA2-Ethylene Responsive Element Binding Protein (AP2-EREBP), NAC (NAM (no apical meristem), ATAF1, 2 (Arabidopsis transcription activation factor), CUC (cup-shaped cotyledon)), and WRKY families, known to be important in defense signaling, revealed changes and phylogenetic rearrangements relative to common bean and soybean that suggest these groups may have evolved different functions. The availability of detailed

  6. Maintenance Trades Guidelines

    Science.gov (United States)

    Weidner, Theodore J.

    2008-01-01

    In 2002, APPA published "Maintenance Staffing Guidelines for Educational Facilities," the first building maintenance trades staffing guideline designed to assist educational facilities professionals with their staffing needs. addresses how facilities professionals can determine the appropriate size and mix of their organization. Contents…

  7. Maintenance improvement program

    International Nuclear Information System (INIS)

    Derbonne, D.R.; Plunkett, T.F.; Simpson, J.R.

    1989-01-01

    During fuel cycle 1 at River Bend station, considerable effort was expended to reduce corrective maintenance work orders (MWOs) to <1,000. This was done by complementing the plant staff with costly contract personnel. Coming out of the first refueling outage, most contract personnel were released. The change in MWO backlog started a steady rise. It became readily apparent that to avoid costly contract staff time, a maintenance improvement program (MIP) was necessary. The MIP Was primarily directed at two areas: crew efficiency improvements and improved preplanned MWO packages. The overall effect of the MIP was to achieve significant productivity improvements with reduced operation and maintenance cost by providing frequent accountability to all levels of maintenance supervision. The MIP also produced a feeling of pride among the maintenance department employees that had not really existed before. This was the best benefit of all

  8. Integrated maintenance program (IMP)

    International Nuclear Information System (INIS)

    Zemdegs, R.T.; Chout, Q.B.

    1998-01-01

    Approaches to the maintenance of nuclear power plants have undergone significant change in the past several decades. The traditional breakdown approach has been displaced by preventive (calendar-based) maintenance and more recently, by condition-based maintenance (CBM). This is largely driven by the fact that traditional maintenance programs, derived primarily from equipment vendor recommendations, are generally unsuccessful in controlling maintenance costs or equipment failures. Many advances in the maintenance field have taken place since the maintenance plans for Ontario Hydro's nuclear plants were initially established. Ontario Hydro nuclear plant operating costs can be substantially reduced and Incapability Factor improved with the application of modern maintenance processes and tools. Pickering is designated as the lead station for IMP. Of immediate concern is the fact that Pickering Nuclear Division has been experiencing a significant backlog of Operating Preventive Maintenance Callups. This backlog, over 2000, is unacceptable to both station management and the nuclear regulator, the Atomic Energy Control Board. In addition there are over 500 callups in various stages of revision (in hyperspace) without an adequate control nor reporting system to manage their completion. There is also considerable confusion about the classification of l icensing c allups, e.g. callups which are mandatory as a result of legal requirements. Furthermore the ineffectiveness of the Preventive Maintenance (PM) has been the subject of peer audits and Atomic Energy Control Board (AECB) findings over the past several years. The current preventive maintenance ratio PM2 /(PM+CM3) at Pickering ND is less than 20%, due to the current high load of equipment breakdown. This past summer, an Independent Integrated Performance Assessment (IIPA) review at Ontario Hydro confirmed these concerns. Over the past several years, Ontario Hydro nuclear staff have evaluated several programs to improve

  9. Maintenance Process Strategic Analysis

    Science.gov (United States)

    Jasiulewicz-Kaczmarek, M.; Stachowiak, A.

    2016-08-01

    The performance and competitiveness of manufacturing companies is dependent on the availability, reliability and productivity of their production facilities. Low productivity, downtime, and poor machine performance is often linked to inadequate plant maintenance, which in turn can lead to reduced production levels, increasing costs, lost market opportunities, and lower profits. These pressures have given firms worldwide the motivation to explore and embrace proactive maintenance strategies over the traditional reactive firefighting methods. The traditional view of maintenance has shifted into one of an overall view that encompasses Overall Equipment Efficiency, Stakeholders Management and Life Cycle assessment. From practical point of view it requires changes in approach to maintenance represented by managers and changes in actions performed within maintenance area. Managers have to understand that maintenance is not only about repairs and conservations of machines and devices, but also actions striving for more efficient resources management and care for safety and health of employees. The purpose of the work is to present strategic analysis based on SWOT analysis to identify the opportunities and strengths of maintenance process, to benefit from them as much as possible, as well as to identify weaknesses and threats, so that they could be eliminated or minimized.

  10. Program integration of predictive maintenance with reliability centered maintenance

    International Nuclear Information System (INIS)

    Strong, D.K. Jr; Wray, D.M.

    1990-01-01

    This paper addresses improving the safety and reliability of power plants in a cost-effective manner by integrating the recently developed reliability centered maintenance techniques with the traditional predictive maintenance techniques of nuclear power plants. The topics of the paper include a description of reliability centered maintenance (RCM), enhancing RCM with predictive maintenance, predictive maintenance programs, condition monitoring techniques, performance test techniques, the mid-Atlantic Reliability Centered Maintenance Users Group, test guides and the benefits of shared guide development

  11. Space station orbit maintenance

    Science.gov (United States)

    Kaplan, D. I.; Jones, R. M.

    1983-01-01

    The orbit maintenance problem is examined for two low-earth-orbiting space station concepts - the large, manned Space Operations Center (SOC) and the smaller, unmanned Science and Applications Space Platform (SASP). Atmospheric drag forces are calculated, and circular orbit altitudes are selected to assure a 90 day decay period in the event of catastrophic propulsion system failure. Several thrusting strategies for orbit maintenance are discussed. Various chemical and electric propulsion systems for orbit maintenance are compared on the basis of propellant resupply requirements, power requirements, Shuttle launch costs, and technology readiness.

  12. Maintenance optimization after RCM

    International Nuclear Information System (INIS)

    Doyle, E.K.; Lee, C.-G.; Cho, D.

    2005-01-01

    Variant forms of RCM (Reliability Centered Maintenance) have been the maintenance optimizing tools of choice in industry for the last 20 years. Several such optimization techniques have been implemented at the Bruce Nuclear Station. Further cost refinement of the Station preventive maintenance strategy whereby decisions are based on statistical analysis of historical failure data are now being evaluated. The evaluation includes a requirement to demonstrate that earlier optimization projects have long term positive impacts. This proved to be a significant challenge. Eventually a methodology was developed using Crowe/AMSAA (Army Materials Systems Analysis Activity) plots to justify expenditures on further optimization efforts. (authors)

  13. Nuclear power plants maintenance

    International Nuclear Information System (INIS)

    Anon.

    1988-01-01

    Nuclear power plants maintenance now appears as an important factor contributing to the competitivity of nuclea energy. The articles published in this issue describe the way maintenance has been organized in France and how it led to an actual industrial activity developing and providing products and services. An information note about Georges Besse uranium enrichment plant (Eurodif) recalls that maintenance has become a main data not only for power plants but for all nuclear industry installations. (The second part of this dossier will be published in the next issue: vol. 1 January-February 1989) [fr

  14. Mobile network maintenance (GSM)

    CERN Multimedia

    IT Department

    2009-01-01

    Maintenance work will be carried out on the CERN mobile network infrastructure (GSM) on the 23 and 24 July from 6 p.m. to 6 a.m. in order to replace discontinued equipment and to increase the bandwidth capacity of the GSM mobile network. All CERN GSM emitters (40 units) will be moved one by one to the new infrastructure during the maintenance. The call of a user connected to an emitter at the time of its maintenance will be cut off. However, the general overlapping of the GSM radio coverage should mean that users are able immediately to call again should their call be interrupted. IT/CS/CS

  15. Initiating statistical maintenance optimization

    International Nuclear Information System (INIS)

    Doyle, E. Kevin; Tuomi, Vesa; Rowley, Ian

    2007-01-01

    Since the 1980 s maintenance optimization has been centered around various formulations of Reliability Centered Maintenance (RCM). Several such optimization techniques have been implemented at the Bruce Nuclear Station. Further cost refinement of the Station preventive maintenance strategy includes evaluation of statistical optimization techniques. A review of successful pilot efforts in this direction is provided as well as initial work with graphical analysis. The present situation reguarding data sourcing, the principle impediment to use of stochastic methods in previous years, is discussed. The use of Crowe/AMSAA (Army Materials Systems Analysis Activity) plots is demonstrated from the point of view of justifying expenditures in optimization efforts. (author)

  16. Plant Maintenance. The Licensee's Viewpoint

    Energy Technology Data Exchange (ETDEWEB)

    Ungi, T. [Heysham 2 Power Station, Nuclear Electric LTD (United Kingdom)

    1997-07-01

    Plant maintenance is a very complex process which requires considerable effort from both within the maintenance process and also many support activities. It is important that the plant maintenance policy is translated into a maintenance programme which will define all relevant aspects of the maintenance. An aspect of this maintenance programme will be a maintenance catalogue which will define the maintenance activities to be carried out and at what frequencies. This paper is aimed at discussing the maintenance philosophy and resulting maintenance catalogues currently adopted in Nuclear Electric Ltd, and in particular at Heysham 2 Power Station. It goes on to consider whether these maintenance catalogues contain the optimum maintenance and if not should they be changed. If change is required, the process by which this change will be brought about is also discussed. (author)

  17. Protein Dynamics in Mammalian Genome Maintenance

    NARCIS (Netherlands)

    A. Zotter (Angelika)

    2008-01-01

    textabstractThe integrity of the genome, carrier of the blueprint for each organism, is under constant attack from environmental as well as endogenous DNA damaging agents. An agent with substantial impact on our DNA is the UV-fraction of sunlight. It inflicts bulky DNA lesions, which can interfere

  18. Experts' meeting: Maintenance '83

    International Nuclear Information System (INIS)

    1983-01-01

    The brochure presents, in full wording, 20 papers read at the experts' meeting ''Maintenance '83'' in Wiesbaden. Most of the papers discuss reliability data (acquisition, evaluation, processing) of nearly all fields of industry. (RW) [de

  19. Excellence through maintenance mastering

    International Nuclear Information System (INIS)

    Lamarche, M.; Guillot, M.; Monier, M.

    1987-01-01

    To improve the overall availability factor of nuclear power plants you have to cut either the forced outage role and the planned outage time or you need to reduce simultaneously the failures and the efforts devoted to avoiding them. Among other results, this goal leads to a decrease in the number of real or anticipated problems. Electricite de France policy, in this matter, is to focus on decreasing the compoents' maintenance needs through a comprehensive modification program, targeted to eliminating the weak points as revealed by the operation. Thereby one may reach, for simple equipment, a nearly maintenance-free condition in which the only maintenance needed is surveillance, which provides assurance that everything is operating properly. A good example of this type of equipment is the family of static components, drums, and pipes whose corrective maintenance is almost nil when their initial condition is good

  20. Remote maintenance development

    International Nuclear Information System (INIS)

    Zook, C.R.

    1979-01-01

    The concept of remote maintenance as it pertains to nuclear fuel fabrication facilities is quite unique. The future may require completely remote facilities where maintenance will be performed by hybrid manipulators/robots. These units will be capable of being preprogrammed for automatic operation or manually operated with the operator becoming a part of the closed loop control system. These robots will mesh television, computer control, and direct force feedback manual control in a usable new concept of robotics

  1. Predictive maintenance primer

    International Nuclear Information System (INIS)

    Flude, J.W.; Nicholas, J.R.

    1991-04-01

    This Predictive Maintenance Primer provides utility plant personnel with a single-source reference to predictive maintenance analysis methods and technologies used successfully by utilities and other industries. It is intended to be a ready reference to personnel considering starting, expanding or improving a predictive maintenance program. This Primer includes a discussion of various analysis methods and how they overlap and interrelate. Additionally, eighteen predictive maintenance technologies are discussed in sufficient detail for the user to evaluate the potential of each technology for specific applications. This document is designed to allow inclusion of additional technologies in the future. To gather the information necessary to create this initial Primer the Nuclear Maintenance Applications Center (NMAC) collected experience data from eighteen utilities plus other industry and government sources. NMAC also contacted equipment manufacturers for information pertaining to equipment utilization, maintenance, and technical specifications. The Primer includes a discussion of six methods used by analysts to study predictive maintenance data. These are: trend analysis; pattern recognition; correlation; test against limits or ranges; relative comparison data; and statistical process analysis. Following the analysis methods discussions are detailed descriptions for eighteen technologies analysts have found useful for predictive maintenance programs at power plants and other industrial facilities. Each technology subchapter has a description of the operating principles involved in the technology, a listing of plant equipment where the technology can be applied, and a general description of the monitoring equipment. Additionally, these descriptions include a discussion of results obtained from actual equipment users and preferred analysis techniques to be used on data obtained from the technology. 5 refs., 30 figs

  2. Maintenance of scientific instruments

    International Nuclear Information System (INIS)

    Lucero, E.

    1986-01-01

    During the last years Colombia has increased the use of nuclear techniques, instruments and equipment in ambitious health programs, as well as in research centers, industry and education; this has resulted in numerous maintenance problems. As an alternative solution IAN has established a Central Maintenance Laboratory for nuclear instruments within an International Atomic Energy Agency program for eight Latin American and nine Asian Countries. Established strategies and some results are detailed in this writing

  3. Mechanical configuration and maintenance

    International Nuclear Information System (INIS)

    Brown, T.G.; Casini, G.; Churakov, G.F.

    1982-01-01

    The INTOR engineering design has been strongly influenced by considerations for assembly and maintenance. A maintenance philosophy was established at the outset of the conceptual design to insure that the tokamak configuration would be developed to accommodate maintenance requirements. The main features of the INTOR design are summarized in this paper with primary emphasis on the impact of maintenance considerations. The most apparent configuration design feature is the access provided for torus maintenance. Particular attention was given to the size and location of superconducting magnets and the location of vacuum boundaries. All of the poloidal field (PF) coils are placed outside of the bore of the toroidal field (TF) coils and located above and below an access opening between adjacent TF coils through which torus sectors are removed. A magnet structural configuration consisting of mechanically attached reinforcing members has been designed which facilitates the open access space for torus sector removal. For impurity control, a single null poloidal divertor was selected over a double null design in order to maintain sufficient access for pumping and maintenance of the collector. A double null divertor was found to severely limit access to the torus with the addition of divertor collectors and pumping at the top. For this reason, a single null concept was selected in spite of the more difficult design problems associated with the required asymmetric PF system and higher particle loadings

  4. Principles of MONJU maintenance. Characteristic of MONJU maintenance and reflection of LWR maintenance experience to FBR

    International Nuclear Information System (INIS)

    Nakai, Satoru; Nishio, Ryuichi; Uchihashi, Masaya; Kaneko, Yoshihisa; Yamashita, Hironobu; Yamaguchi, Atsunori; Aoki, Takayuki

    2014-01-01

    A sodium cooled fast breeder reactor (FBR) has unique systems and components and different degradation mechanism from light water reactor (LWR) so that need to establish maintenance technology in accordance with its features. The examination of the FBR maintenance technology is carried out in the special committee for considering the maintenance for Monju established in the Japan Society of Maintenology (JSM). As a result of the study such as extraction of Monju maintenance feature, maintenance technology benchmark between Monju and LWR components and survey of LWR maintenance experience, it is clear that principles of maintenance are same as LWR, necessity of LWR maintenance experience reflection and points to be considered in Monju maintenance. The road map to establish a FBR maintenance technology in the technical aspect became clear and it is vital to acquire operation and maintenance experience of the plant to implement this road map, and to establish a fast reactor maintenance. (author)

  5. Building relationships for better maintenance.

    Science.gov (United States)

    Lee, Hackman Hon Yin; Scott, David

    2009-02-01

    Effective management of building maintenance is a vital ingredient in ensuring a high quality built environment for any building's occupiers. However, maintenance is not high on the list of priorities for most organisations. Communication between top management personnel looking at maintenance issues from a strategic standpoint and maintenance staff considering them at an operational level is often not as good as it should be. When planning maintenance activities maintenance personnel often draw too heavily on their technical experience and expertise without taking sufficient account of wider organisational objectives or consulting effectively with top management. Senior managers also regularly complain about lack of managerial input from maintenance departments. Such barriers contribute to communication difficulties between top management at a strategic level and maintenance personnel at an operational level. Identifying where the key differences lie in senior managers' and maintenance personnel's viewpoints on maintenance strategy can prove invaluable in achieving some convergence of opinions and optimising the efficiency of the overall building maintenance process.

  6. JRR-3 maintenance program utilizing accumulated maintenance data

    International Nuclear Information System (INIS)

    Izumo, Hironobu; Kato, Tomoaki; Kinase, Masami; Torii, Yoshiya; Murayama, Yoji

    2007-07-01

    JRR-3(Japan Research Reactor No.3) has been operated for more than 15 years after the modification, without significant troubles by carrying out maintenance such as the preventive maintenance (mainly time-based maintenance) for the safety-grade equipments and the breakdown maintenance for the non-safety-grade equipments. Unscheduled shutdowns causes by aged non-safety-grade equipments have been increasing, and the resources such as budgets have been decreasing year by year. In this situation, JRR-3 maintenance program was reviewed about safety, reliability and economic efficiency. This report offers the policy of the maintenance review and the future direction of maintenance programs. (author)

  7. Maintenance for life management

    International Nuclear Information System (INIS)

    Hevia, F.

    1997-01-01

    Life Management is based on the detection, monitoring and control of long-term degradation that affects individual plant components and important populations. Experience has shown that in many cases current maintenance practices do not attend to ageing directly; instead they deal with the consequences when it is already too late, when good Life Management is no longer practical. This has brought about the need for specific Maintenance Evaluation and Improvement Programmes to adjust to the basic objective of Life Management which is to project against, monitor and mitigate ageing that can affect the safe and profitable operating life of the facility. New regulatory requirements for ageing monitoring and effective maintenance to ensure safety (Maintenance Rule) have made it even more necessary to implement the Maintenance Evaluation Programme to cope with ageing, and to integrate that tasks in both programmes to optimise effort and use of tools. This paper presents a brief description of the objectives and methodologies of these Programmes which has been applied to plants around the world and in Spain at the Garona and Vandellos II plants in Spain as part of the PIE project for developing a Remanent Life Evaluation System for nuclear power plants. (Author)

  8. Building Maintenance, Management, and Budgeting.

    Science.gov (United States)

    Pawsey, M. R.

    1982-01-01

    Australian methods and formulas for funding building maintenance and management are outlined and found to be haphazard. Discussed are: ultimate costs of deferred maintenance, major plant replacements, life cycle costing, types of maintenance programs (including full preventive maintenance), use of computer programs for planning, and organization…

  9. The MERC maintenance system

    International Nuclear Information System (INIS)

    Izquierdo, J.J.; Chauvire, P.; Plessis, L.

    1991-01-01

    Maintenance in the modern commercial reprocessing plants is a subject of the highest priority in order to guarantee availability for the whole life of the plant. The design concept of the UP3 reprocessing plant at La Hague identified several categories of equipment according to their maintenance design principles. Among them, Standard Process Equipment such as pumps, valves, ejectors and filters which need periodic maintenance. These equipment items are designed in removable modular form to avoid the need to disconnect piping. Removal is performed under shielding and without breach of containment through the use of a transfer cask that is connected to the cell containing the equipment to be removed. This transfer cask is called MERC (Mobile Equipment Replacement Cask). Containment is preserved by an air-tight system consisting of coupled doors, with a special connecting gasket. This system can remove and replace failed equipment, without spreading contamination to work areas. (author)

  10. Reliability Centered Maintenance - Methodologies

    Science.gov (United States)

    Kammerer, Catherine C.

    2009-01-01

    Journal article about Reliability Centered Maintenance (RCM) methodologies used by United Space Alliance, LLC (USA) in support of the Space Shuttle Program at Kennedy Space Center. The USA Reliability Centered Maintenance program differs from traditional RCM programs because various methodologies are utilized to take advantage of their respective strengths for each application. Based on operational experience, USA has customized the traditional RCM methodology into a streamlined lean logic path and has implemented the use of statistical tools to drive the process. USA RCM has integrated many of the L6S tools into both RCM methodologies. The tools utilized in the Measure, Analyze, and Improve phases of a Lean Six Sigma project lend themselves to application in the RCM process. All USA RCM methodologies meet the requirements defined in SAE JA 1011, Evaluation Criteria for Reliability-Centered Maintenance (RCM) Processes. The proposed article explores these methodologies.

  11. Computer assisted procedure maintenance

    International Nuclear Information System (INIS)

    Bisio, R.; Hulsund, J. E.; Nilsen, S.

    2004-04-01

    The maintenance of operating procedures in a NPP is a tedious and complicated task. Through the whole life cycle of the procedures they will be dynamic, 'living' documents. Several aspects of the procedure must be considered in a revision process. Pertinent details and attributes of the procedure must be checked. An organizational structure must be created and responsibilities allotted for drafting, revising, reviewing and publishing procedures. Available powerful computer technology provides solutions within document management and computerisation of procedures. These solutions can also support the maintenance of procedures. Not all parts of the procedure life cycle are equally amenable to computerized support. This report looks at the procedure life cycle in todays NPPs and discusses the possibilities associated with introduction of computer technology to assist the maintenance of procedures. (Author)

  12. Maintenance simulation: Software issues

    Energy Technology Data Exchange (ETDEWEB)

    Luk, C.H.; Jette, M.A.

    1995-07-01

    The maintenance of a distributed software system in a production environment involves: (1) maintaining software integrity, (2) maintaining and database integrity, (3) adding new features, and (4) adding new systems. These issues will be discussed in general: what they are and how they are handled. This paper will present our experience with a distributed resource management system that accounts for resources consumed, in real-time, on a network of heterogenous computers. The simulated environments to maintain this system will be presented relate to the four maintenance areas.

  13. Training of maintenance personnel

    International Nuclear Information System (INIS)

    Rabouhams, J.

    1986-01-01

    This lecture precises the method and means developed by EDF to ensure the training of maintenance personnel according to their initial educational background and their experience. The following points are treated: General organization of the training for maintenance personnel in PWR and GCR nuclear power stations and in Creys Malville fast breeder reactor; Basic nuclear training and pedagogical aids developed for this purpose; Specific training and training provided by contractors; complementary training taking into account the operation experience and feedback; Improvement of velocity, competence and safety during shut-down operations by adapted training. (orig.)

  14. Maintenance and environmental qualification

    International Nuclear Information System (INIS)

    Martin, R.S.; Austin, D.G.

    1995-01-01

    The design of today's nuclear generating plants involves many detailed design considerations. This includes comprehensive look at aging effects on plant components over their expected lifetimes. This is important to ensuring that the plant operates safely throughout its life. The effects of aging are required to be documented in detail in today's designs. This documentation provides assurance that safe operating conditions are maintained throughout the station life cycle. This requirement is analogous to the longer standing requirement to ensure pressure boundary integrity. The pressure boundary integrity requirement has existed in the industry since its inception. The subject of plant aging effects and the maintenance of functionality is known as Environmental Qualification (EQ). This paper will attempt to explain the wisdom of EQ and the potential for optimizing maintenance activities (to move from reactive to proactive activities), within the context of the overall maintenance program. It is the author's intent to encourage the active involvement of maintenance professionals in the effective implementation of the ongoing EQ program so that the benefits are maximized

  15. Maintenance Resources Evaluation Technique.

    Science.gov (United States)

    1999-03-01

    variable Z; P-6 BIBLIOGRAPHY Argentine Air Force. Reglamento de Conduction Logistica RAC 9 (Logistics Management Regulation). Estado Mayor General de ...other hand, too many resources are expensive to acquire and maintain and difficult to transport . 1-1 Sizing the means needed to accomplish its...functional areas (maintenance, supply/inventory, transportation , etc), methodologies of operations research (simulation, mathematical programming

  16. Optimizing preventive maintenance

    International Nuclear Information System (INIS)

    DiCola, F.E.

    1988-01-01

    The traditional approach to preventive maintenance (PM) is based on adherence to manufacturers's requirements and recommended frequencies. When equipment fails, either new procedures are established or the frequency is increased. This leads to an increase in the number of PM activities, overloading not only maintenance resources, but support as well. There is no correlation between more PM and an increase in equipment reliability. More PM may actually induce failures. Reliability-centered maintenance, a new concept in utility maintenance, is based on identifying system/subsystem functions, failures, and dominant failure modes to develop or revise PM tasks. The activities described in this paper are based on actual implementation of this concept on an ongoing project to upgrade the PM program at one of the largest electric utilities in the country. Optimum PM activities are those that, when implemented, will minimize factors that c cause equipment to fail. One technique described illustrates how equipment performance, failure modes, and causes can be related to minimize the occurrence of failures. Operating history and service life of a component are key factors in determining the most effective PM activities, provided that the factors are related to failure modes and causes

  17. Floors: Care and Maintenance.

    Science.gov (United States)

    Post Office Dept., Washington, DC.

    Guidelines, methods and policies regarding the care and maintenance of post office building floors are overviewed in this handbook. Procedures outlined are concerned with maintaining a required level of appearance without wasting manpower. Flooring types and characteristics and the particular cleaning requirements of each type are given along with…

  18. Floors: Selection and Maintenance.

    Science.gov (United States)

    Berkeley, Bernard

    Flooring for institutional, commercial, and industrial use is described with regard to its selection, care, and maintenance. The following flooring and subflooring material categories are discussed--(1) resilient floor coverings, (2) carpeting, (3) masonry floors, (4) wood floors, and (5) "formed-in-place floors". The properties, problems,…

  19. Maintenance procedure upgrade programs

    International Nuclear Information System (INIS)

    Campbell, J.J.; Zimmerman, C.M.

    1988-01-01

    This paper describes a systematic approach to upgrading nuclear power plant maintenance procedures. The approach consists of four phases: diagnosis, program planning, program implementation, and program evaluation. Each phase is explained as a series of steps to ensure that all factors in a procedure upgrade program are considered

  20. Maintenance: problem and solution

    CERN Multimedia

    CERN Bulletin

    2012-01-01

    Equipment that is often unique, machines that are as old as the Laboratory, continuous and demanding performance requirements: these are the challenges faced by CERN’s accelerator maintenance teams. There are some twenty such teams, attached to different departments. A new project aims to standardise their procedures to make their work easier, and you can be a part of it.   “For the past year or so, the Accelerator and Technologies Sector and the GS Department have been working together on identifying the needs of the different teams that perform maintenance on CERN’s equipment. We are now ready to provide computer support with detailed specifics about the processes that need to be set up,” explains Goran Perinić, one of the leaders in CERN’s new Maintenance Management project (MMP). Since the LHC entered operation, the responsibilities of the technical teams have been broadened to cover maintenance of the collider and that of its injectors...

  1. Subcontracting Railway Maintenance

    DEFF Research Database (Denmark)

    Thommesen, Jacob

    2010-01-01

    railway infrastructure manager (InfraMan), whichis subcontracting maintenance tasks to other companies. InfraMan has implemented various central elements in managing their subcontractors. The latter are required to present a safety plan as part of their bid, they must be approved if they are to be awarded...

  2. Operations and maintenance philosophy

    International Nuclear Information System (INIS)

    DUNCAN, G.P.

    1999-01-01

    This Operations and Maintenance (O and M) Philosophy document is intended to establish a future O and M vision, with an increased focus on minimizing worker exposure, ensuring uninterrupted retrieval operations, and minimizing operation life-cycle cost. It is intended that this document would incorporate O and M lessons learned into on-going and future project upgrades

  3. Aircraft Maintenance Expert Systems.

    Science.gov (United States)

    1983-11-01

    PARA 2 -104)) 44: (( JETCAL ANALYSIS SHOWS SYSTEM READS CORRECT) (REPLACE FAULTY PARTS)) 45: ((OVERTEMP EXCEEDED SERVICE LIMITS) 46: I(ENGINE CONTROL...CIRCUITS WITHIN LIMITS ON JETCAL ) (REPLACE FAULTY PARTS)) 47: (ADJUST EST AT AMPLIFIER AND CHECK TENP)) (SEND ENGINE TO HIGHER LEVEL MAINTENANCE)) 48: 2

  4. Telephone Exchange Maintenance

    CERN Multimedia

    2005-01-01

    Urgent maintenance work on CERN telephone exchanges will be performed on 24 March from 6 a.m. to 8 a.m. Telephone services may be disrupted or even interrupted during this time. For more details, please contact us by email at Standard.Telephone@cern.ch.

  5. CH Packaging Maintenance Manual

    International Nuclear Information System (INIS)

    Washington TRU Solutions

    2002-01-01

    This procedure provides instructions for performing inner containment vessel (ICV) and outer containment vessel (OCV) maintenance and periodic leakage rate testing on the following packaging seals and corresponding seal surfaces using a nondestructive helium (He) leak test. In addition, this procedure provides instructions for performing ICV and OCV structural pressure tests

  6. Maintenance: Two Views.

    Science.gov (United States)

    Ballard, J. Barry; Burdette, Clarence E.

    1987-01-01

    Presents two viewpoints on the question of using funding from the Vocational Education Act to maintain ongoing vocational programs. Ballard argues that programs have improved qualitatively because of additional program improvement monies, whereas Burdette argues that the ban on using funds for maintenance treats some states unfairly. (CH)

  7. Maintenance and hazardous substances

    NARCIS (Netherlands)

    Kuhl, K.; Terwoert, J.; Cabecas, J.J.M.

    2012-01-01

    Maintenance workers come into close contact with a broad variety of often hazardous chemicals. Depending on the specific type, these chemicals may not only cause diseases like skin sores or cancer, but many of them are highly flammable and explosive. This e-facts focuses on the specific risks

  8. Building Maintenance Syllabus.

    Science.gov (United States)

    Fischer, Joseph; Messier, Joseph

    Building maintenance is a basic two-year trade education course requiring 2 1/2 hours of study on each of 160 teaching days per year. Student abilities should range from those capable of the simplest custodial work to those who may eventually be superintendents of building complexes. The syllabus is organized in sections by traditional skills…

  9. Maintenance and regulations

    International Nuclear Information System (INIS)

    Noel, R.

    1984-01-01

    Description of the main regulations concerning the processes tied with maintenance and in service supervision of the pressure vessels in classical or nuclear power plants or of their accessories (essentially in order to fix the time-table of the hydraulic test procedures and the inspection chronology [fr

  10. Maintenance in sustainable manufacturing

    Directory of Open Access Journals (Sweden)

    Vladimir Stuchly

    2014-09-01

    Full Text Available Background: Sustainable development is about reaching a balance between economic, social, and environmental goals, as well as people's participation in the planning process in order to gain their input and support. For a company, sustainable development means adoption of such business strategy and actions that contribute to satisfying present needs of company and stakeholders, as well as simultaneous protection, maintenance and strengthening of human and environmental potential which will be needed in the future. This new approach forces manufacturing companies to change their previous management paradigms. New management paradigm should include new issues and develop innovative methods, practices and technologies striving for solving problem of shortages of resources, softening environment overload and enabling development of environment-friendly lifecycle of products. Hence, its realization requires updating existing production models as they are based on previously accepted paradigm of unlimited resources and unlimited regeneration capabilities. Maintenance plays a crucial role because of its impact on availability, reliability, quality and life cycle cost, thus it should be one of the main pillars of new business running model.  Material and methods: The following paper is a result of research on the literature and observation of practices undertaken by a company within maintenance area. Results and conclusions: The main message is that considering sustainable manufacturing requires considerable expanding range of analysis and focusing on supporting processes. Maintenance offers numerous opportunities of decreasing influence of business processes on natural environment and more efficient resources utilization. The goal of maintenance processes realizing sustainable development strategy is increased profitability of exploitation and optimization of total lifecycle cost without disturbing safety and environmental issues. 

  11. Advances in safety related maintenance

    International Nuclear Information System (INIS)

    2000-03-01

    The maintenance of systems, structures and components in nuclear power plants (NPPs) plays an important role in assuring their safe and reliable operation. Worldwide, NPP maintenance managers are seeking to reduce overall maintenance costs while maintaining or improving the levels of safety and reliability. Thus, the issue of NPP maintenance is one of the most challenging aspects of nuclear power generation. There is a direct relation between safety and maintenance. While maintenance alone (apart from modifications) will not make a plant safer than its original design, deficient maintenance may result in either an increased number of transients and challenges to safety systems or reduced reliability and availability of safety systems. The confidence that NPP structures, systems and components will function as designed is ultimately based on programmes which monitor both their reliability and availability to perform their intended safety function. Because of this, approaches to monitor the effectiveness of maintenance are also necessary. An effective maintenance programme ensures that there is a balance between the improvement in component reliability to be achieved and the loss of component function due to maintenance downtime. This implies that the safety level of an NPP should not be adversely affected by maintenance performed during operation. The nuclear industry widely acknowledges the importance of maintenance in NPP safety and operation and therefore devotes great efforts to develop techniques, methods and tools to aid in maintenance planning, follow-up and optimization, and in assuring the effectiveness of maintenance

  12. Perencanaan Kegiatan Maintenance Dengan Metode Reability Centered Maintenance (Rcm) II

    OpenAIRE

    Rachmad Hidayat; Nachnul Ansori; Ali Imron

    2010-01-01

    Maintenance Activity Planning by Reability Centered Maintenance (RCM) II Method. This research discussmaintenance activity by using RCM II method to determine failure function risk at compresor screw. Calculation isgiven to magnitude optimum time maintenance interval by considering the cost maintenance and the cost reparation.From the research results with RPN points out that critical component that needs to get main priority in givemaintenance on compresor screw are bust logistic on timeworn...

  13. Interval of Routine Maintenance and Maintenance Performance: A Literature Review

    Directory of Open Access Journals (Sweden)

    Au-Yong Cheong Peng

    2016-01-01

    Full Text Available In high-rise residential buildings, the quality of facilities management services is significant to the normal operation of the facilities. Unfortunately, lack of concern towards building maintenance, especially preventive maintenance, happens in domestic housing industry in Malaysia. Majority of the maintenance operations of condominiums suffer from lack of planning, lack of proactive maintenance plan, and lack of proper implementation. Thus, this paper reviews the implementation of preventive maintenance strategy, routine maintenance in specific. An extensive review of literature published in 1987 to 2014 is performed for the purpose of this research. The publications are sourced from journal articles, conference proceedings and books. The literature analysis confirms that the routine maintenance of facilities and building services is vital and it can be influential towards the maintenance performance. Subsequently, a theoretical framework is developed, which shows the relationship between routine maintenance of building facilities & services and maintenance performance. The building facilities & services are divided into two categories. They are essential facilities & services that ensure the safety, health, habitability, and operability of buildings; while value-added facilities & services deal with property value, return on investment, and quality living of buildings. Based on the findings, a future research is proposed, which aims to identify the appropriate routine of maintenance for the facilities and services in high-rise residential buildings to improve the maintenance performance.

  14. Selection of maintenance tasks

    Energy Technology Data Exchange (ETDEWEB)

    Dean, B; Rombos, P [Wardrop (W.L.) and Associates Ltd., Winnipeg, MB (Canada)

    1995-10-01

    Two methodologies for maintenance task selection, Reliability Centre Maintenance (RCM) and Degradation Mode Analysis (DMA), are compared with regard to application in the nuclear industry and potential for application at CANDU nuclear power plants. RCM is the favoured one of the two methodologies. It is more thorough than DMA, is well supported within the US nuclear industry, and - with experience in application - is gaining cost effectiveness. There is interest in the use of RCM in other nations, including France and Japan, and it is already being implemented at Bruce A NGS and Bruce B NGS in Canada. DMA lags behind RCM in development and currently there is little experience to support claims of major benefits at reduced cost. Significant advantages over RCM need to be demonstrated if DMA is to gain acceptance in the nuclear industry. (author). 41 refs., 7 tabs., 8 figs.

  15. Maintenance - a design perspective

    International Nuclear Information System (INIS)

    Hedges, K.R.

    1992-01-01

    The paper describes how the CANDU-3 has incorporated, at the design stage, maintenance requirements such as component accessibility, standardization, ease of replacement, reduction in the number of components, and simplified component design. AECL is also active in the planned rehabilitation of the four units at Ontario Hydro's Bruce A nuclear generating station. In addition to conceptual design and development, detailed design and analysis, procurement and site assistance, AECL's Sheridan Park Laboratory will provide important development and mockup facilities. An AECL research and development program on reactor maintenance includes the identification and characterization of aging and degrading mechanisms, the tailoring of developed products to plant requirements, and the identification of areas of international cooperation and information exchange. 3 tabs., 1 fig

  16. Selection of maintenance tasks

    International Nuclear Information System (INIS)

    Dean, B.; Rombos, P.

    1995-10-01

    Two methodologies for maintenance task selection, Reliability Centre Maintenance (RCM) and Degradation Mode Analysis (DMA), are compared with regard to application in the nuclear industry and potential for application at CANDU nuclear power plants. RCM is the favoured one of the two methodologies. It is more thorough than DMA, is well supported within the US nuclear industry, and - with experience in application - is gaining cost effectiveness. There is interest in the use of RCM in other nations, including France and Japan, and it is already being implemented at Bruce A NGS and Bruce B NGS in Canada. DMA lags behind RCM in development and currently there is little experience to support claims of major benefits at reduced cost. Significant advantages over RCM need to be demonstrated if DMA is to gain acceptance in the nuclear industry. (author). 41 refs., 7 tabs., 8 figs

  17. Flight Crew Health Maintenance

    Science.gov (United States)

    Gullett, C. C.

    1970-01-01

    The health maintenance program for commercial flight crew personnel includes diet, weight control, and exercise to prevent heart disease development and disability grounding. The very high correlation between hypertension and overweight in cardiovascular diseases significantly influences the prognosis for a coronary prone individual and results in a high rejection rate of active military pilots applying for civilian jobs. In addition to physical fitness the major items stressed in pilot selection are: emotional maturity, glucose tolerance, and family health history.

  18. Predictors of weight maintenance

    NARCIS (Netherlands)

    Pasman, W.J.; Saris, W.H.M.; Westerterp-Plantenga, M.S.

    1999-01-01

    Objective: To obtain predictors of weight maintenance after a weight-loss intervention. Research Methods and Procedures: An overall analysis of data from two-long intervention studies [n = 67 women; age: 37.9±1.0 years; body weight (BW): 87.0±1.2 kg; body mass index: 32.1±0.5 kg·m-2; % body fat:

  19. Maintenance, a global approach

    International Nuclear Information System (INIS)

    Calmejane, P.

    2001-01-01

    Maintenance is an important economic activity, it can represent up to 40% of operating costs in some fabrication units. The opening of energy markets to competition has led main operators to center their activities on their basis business and to sub-contract subsidiary activities. Subcontracting with strong engagements from the sub-contractor about results, time allowed and costs is now currently applied in nuclear industry. (A.C.)

  20. Computerized plant maintenance management

    International Nuclear Information System (INIS)

    Kozusko, A.M.

    1986-01-01

    The evolution of the computer has and continues to have a great impact on industry. We are in an adjustment cycle with the current computer evolution, and will need to adapt to make the changes for the coming decade. Hardware and software are continually being enhanced. Computers are becoming more powerful and will eventually provide an effective man-machine interface. This paper shares experiences encountered during implementations of computerized maintenance systems

  1. Data management in maintenance outsourcing

    International Nuclear Information System (INIS)

    Murthy, D.N.P.; Karim, M.R.; Ahmadi, A.

    2015-01-01

    Most businesses view maintenance as tasks carried out by technicians and the data collected is mostly cost related. There is a growing trend towards outsourcing of maintenance and the data collection issues are not addressed properly in most maintenance service contracts. Effective maintenance management requires proper data management - data collection and analysis for decision-making. This requires a proper framework and when maintenance is outsourced it raises several issues and challenges. The paper develops a framework for data management when maintenance is outsourced and looks at a real case study that highlights the need for proper data management. - Highlights: • Framework for data management in maintenance outsourcing. • Black-box to grey-box approaches for modelling. • Improvements to maintenance decision-making. • Case study to illustrate the approaches and the shortcomings in data collection

  2. An experimental maintenance management system.

    Science.gov (United States)

    1979-01-01

    The purpose of this study was to evaluate Virginia's maintenance management system and to recommend modifications directed at improving it. The study revealed that (1) the current system of allocating maintenance monies is based upon centerline milea...

  3. The TFTR maintenance manipulator

    International Nuclear Information System (INIS)

    Kungl, D.; Loesser, D.; Heitzenroeder, P.; Cerdan, G.

    1989-01-01

    TFTR plans to begin D-T experiments in mid 1990. The D-T experimental program will produce approximately one hundred shots, with a neutron generation rate of 10 19 neutrons per shot. This will result in high levels of activation in TFTR, especially in the vacuum vessel. The primary purpose of the Maintenance Manipulator is to provide a means of remotely performing certain defined maintenance and inspection tasks inside the vacuum torus so as to minimize personnel exposure to radiation. The manipulator consists of a six-link folding boom connected to a fixed boom on a movable carriage. The entire manipulator is housed in a vacuum antechamber connected to the vacuum torus, through a port formerly used for a vacuum pumping duct. The configuration extends 180 0 in either direction to provide complete coverage of the torus. The four 3500 l/s turbopumps which were formerly used in the pumping duct will be mounted on the antechamber. The manipulator will utilize two end effectors. The first, called a General Inspection Arm (GIA) provides a movable platform to an inspection camera and an in-vacuum leak detector. The second is a bilateral, force-reflecting pair of slave arms which utilize specially developed tools to perform several maintenance functions. All components except the slave arms are capable of operating in TFTR's vacuum environment and during 150 0 C bakeout of the torus. (orig.)

  4. Reducing maintenance costs

    International Nuclear Information System (INIS)

    Zaiss, W.; Reuschle, K.; Baier, B.

    2002-01-01

    The increasingly more expensive maintenance measures, cuts in the cost budget, and the loss of know-how on the part of vendors all require a change of policy with respect to maintenance concepts of the part of operators. This also applies to the existing valve concepts, the drives included. Under these aspects, the current drive, which is self-actuated and actuated by outside media, for a parallel-plate valve of a nomial width of 700 was reconsidered. The effort served to reduce maintenance costs and, at the same time, simplify the drive concept as well as cut back on the number of in-service inspections. Moreover, the number of active components were to be minimized and installation conditions in the plant were to be improved. When the boundary conditions to be observed with respect to process technology had been laid down, the competent technical department developed a concept of modification of the drive. A major constituent part was the demonstration of the functioning capability of the new drive under accident conditions. It was achieved mainly by an analytical approach. In the resultant drive concept, the same control valves are employed to actuate a driving cylinder by means of self-actuation or by an outside medium as a function of pressure. (orig.) [de

  5. Maintenance in dental implants

    Directory of Open Access Journals (Sweden)

    Giselle Póvoa Gomes

    2008-01-01

    Full Text Available In implants, maintenance is a decisive factor for obtaining success when implant supported overdentures and dentures are used. The present stud presents, a clinical case of a patient, a 70 year-old white man, with a completely edentulous mandibular alveolar ridge, severe bone resorption with presence of basal bone only, and absence of vestibule. Initially, treatment consisted of the placement of a mandibular overdenture, supported on three implants in the anterior inter-foramen region, as the left implant was transfixed in the basal bone of 2 to 3 millimeters. Eleven years later, another two implants were placed in the anterior area and an immediate load was performed up to the first molars, for the placement of an implant supported fixed. Throughout the entire treatment, meticulous maintenance was carried out, with follow-up for fourteen years, interrupted by the patient’s death. From the third month after the opening the three implants initially placed, the presence of keratinized mucosa, definition of the vestibule, maturation of the alveolar ridge and bone formation in the mento region were observed. It was concluded that good planning, allied to mastery of the technique and adequate maintenance were the prerequisites necessary for obtaining favorable results, success of the present case, and for the patient to have a better quality of life.

  6. Managed maintenance, the next step in power plant maintenance

    International Nuclear Information System (INIS)

    Butterworth, G.; Anderson, T.M.

    1984-01-01

    The Westinghouse Nuclear Services Integration Division managed maintenance services are described. Essential to the management and control of a total plant maintenance programme is the development of a comprehensive maintenance specification. During recent years Westinghouse has jointly developed total plant engineering-based maintenance specifications with a number of utilities. The process employed and the experience to date are described. To efficiently implement the maintenance programme Westinghouse has developed a computer software program specifically designed for day to day use at the power plant by maintenance personnel. This program retains an equipment maintenance history, schedules maintenance activities, issues work orders and performs a number of sophisticated analyses of the maintenance backlog and forecast, equipment failure rates, etc. The functions of this software program are described and details of Westinghouse efforts to support the utilities in reducing outage times through development of predefined outage plans for critical report maintenance activities are given. Also described is the experience gained in the training of specialized maintenance personnel, employing competency-based training techniques and equipment mock-ups, and the benefits experienced, in terms of improved quality and productivity of maintenance performed. The success experienced with these methods has caused Westinghouse to expand the use of these training techniques to the more routine skill areas of power plant maintenance. A significant reduction in the operating costs of nuclear power plants will only be brought about by a significant improvement in the quality of maintenance. Westinghouse intends to effect this change by expanding its international service capabilities and to make major investments in order to promote technological developments in the area of power plant maintenance. (author)

  7. PREVENTIVE MAINTENANCE. HONEYWELL PLANNING GUIDE.

    Science.gov (United States)

    Honeywell, Inc., Minneapolis, Minn.

    THIS HONEYWELL PAMPHLET DISCUSSES SOME ASPECTS OF PREVENTIVE MAINTENANCE OF AUTOMATIC CONTROLS, HEATING, VENTILATING, AND AIR CONDITIONING, AND COMPARES IN-PLANT WITH CONTRACT SERVICE, CONCLUDING THAT CONTRACT SERVICE IS PREFERABLE AND DESCRIBING A NUMBER OF MAINTENANCE PLANS WHICH THEY FURNISH. PREVENTIVE MAINTENANCE PROVIDES--(1) MORE EFFICIENT…

  8. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to casein protein hydrolysates and growth or maintenance of muscle mass (ID 1498), increase in endurance performance (ID 660, 1497) and faster recovery from muscle

    DEFF Research Database (Denmark)

    Tetens, Inge

    Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to casein protein hydrolysates and growth or maintenance of muscle mass, increase in endurance performance and faster recovery from muscle fatigue after exercise. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article...

  9. Motorway maintenance work

    CERN Multimedia

    2005-01-01

    Built 20 years ago, the airport section of the Geneva motorway bypass is now in need of maintenance work and alterations to bring it into line with the new standards. Two-lane traffic will be maintained in both directions during the work, which will be carried out in 2006 and 2007, but there will be various temporary special traffic arrangements in force. We should like to thank you in advance for your understanding. Civil Engineering Department, DAEL, State of Geneva More information: tel. 163 or www.autoroute-aeroport.ch Fewer cars, fewer traffic jams, with www.covoiturage.ch

  10. Managing and controlling maintenance

    International Nuclear Information System (INIS)

    Chavez, G.; Perla, H.F.

    1980-01-01

    A well-planned maintenance-management program can bring organized yet flexible scheduling, improved productivity, and a ready-access data bank. The system detailed in the article, developed for a nuclear power plant, has application to any equipment-intensive facility. Key features of the system include: documentation of all work requirements and their completion; automated prioritizing of work orders; automatic generation of work orders for repetitive work, when due; work lists in priority sequence; deferred, standby, and outage work reports; labor and work-order backlog information; equipment work histories and cumulative failure data

  11. Field installation and maintenance

    International Nuclear Information System (INIS)

    Prebble, R.E.

    1981-01-01

    The use of a neutron moisture meter for the determination of the water content of soil necessitates the installation of an access tube down which the probe can be lowered. Errors in neutron count rate will be introduced if water enters the air gap between a loose fitting tube and the soil. Errors may also be caused by changes in the soil structure resulting from the techniques for soil removal and from driving the access hole. Access tube materials and dimensions, methods of installation and maintenance of the site are discussed

  12. Paranal maintenance and CMMS experience

    Science.gov (United States)

    Montano, Nelson

    2004-10-01

    During the last four years of operations, low technical downtime has been one of the relevant records of the Paranal Observatory. From the beginning of the Very Large Telescope (VLT) project, European Southern Observatory (ESO) has considered the implementation of a proper maintenance strategy a fundamental point in order to ensure low technical down time and preserve the Observatory's assets. The implementation of the maintenance strategy was based on the following aspects: - Strong maintenance sense during the design stage. Line Replacement Unit (LRU) concept, standardization and modularity of the Observatory equipment - Creation of a dedicated team for Maintenance - The implementation of a Computerized Maintenance Management System After four operational years, the result of these aspects has exceeded the expectations; the Observatory has been operating with high availability under a sustainable strategy. The strengths of the maintenance strategy have been based on modern maintenance concepts applied by regular production companies, where any minute of down time involves high cost. The operation of the actual Paranal Maintenance System is based mainly on proactive activities, such as regular inspections, preventive maintenance (PM) and predictive maintenance (PdM) plans. Nevertheless, it has been necessary to implement a strong plan for corrective maintenance (CM). The Spare Parts Strategy has also been an important point linked to the Maintenance Strategy and CMMS implementation. At present, almost 4,000 items related to the Observatory spare parts are loaded into the CMMS database. Currently, we are studying the implementation of a Reliability Centered Maintenance (RCM) project in one of our critical systems The following document presents the actual status of the Paranal Maintenance Strategy and which have been the motivations to implement the established strategy.

  13. Maintenance and management system

    International Nuclear Information System (INIS)

    Ando, Yasumasa.

    1992-01-01

    Since highly reliable operation is required in a nuclear power plant, monitoring during operation and periodical inspection are conducted carefully. The present invention provides maintenance and management systems for providing an aid so that these systems are combined effectively and operated rationally based on unified information management. That is, the system contains data bases comprising information for the design of the equipments and pipelines of a plant, information for the exchange of equipment parts, information for the history of plant operation, information for the monitoring and inspection, and information for the management of repair operation. In addition, it has an equipment part history management sub-system for managing equipment part exchange information, an operation history management sub-system for managing the operation state of the plant, an operation history management sub-system for managing equipment monitoring inspection data and operation management sub-system for managing periodical inspection/ repairing operation. These sub-systems are collectively combined to manage the maintenance and management jobs of the plant unitarily. (I.S.)

  14. ITER assembly and maintenance

    International Nuclear Information System (INIS)

    Honda, T.; Davis, F.; Lousteau, D.

    1991-01-01

    This document is intended to describe the work conducted by the ITER Assembly and Maintenance (A and M) Design Unit and the supporting home teams during the ITER Conceptual Design Activities, carried out from 1988 through 1990. Its content consists of two main sections, i.e., Chapter III, which describes the identified tasks to be performed by the A and M system and a general description of the required equipment; and Chapter IV, which provides a more detailed description of the equipment proposed to perform the assigned tasks. A two-stage R and D program is now planned, i.e., (1) a prototype equipment functional tests using full scale mock-ups and (2) a full scale integration demonstration test facility with real components (vacuum vessel with ports, blanket modules, divertor modules, armor tiles, etc.). Crucial in-vessel and ex-vessel operations and the associated remote handling equipment, including handling of divertor plates and blanket modules will be demonstrated in the first phase, whereby the database needed to proceed with the engineering phase will be acquired. The second phase will demonstrate the ability of the overall system to execute the required maintenance procedures and evaluate the performance of the prototype equipment

  15. Long-term weight maintenance and cardiovascular risk factors are not different following weight loss on carbohydrate-restricted diets high in either monounsaturated fat or protein in obese hyperinsulinaemic men and women.

    Science.gov (United States)

    Keogh, Jennifer B; Luscombe-Marsh, Natalie D; Noakes, Manny; Wittert, Gary A; Clifton, Peter M

    2007-02-01

    The aim of this study was to determine after 52 weeks whether advice to follow a lower carbohydrate diet, either high in monounsaturated fat or low fat, high in protein had differential effects in a free-living community setting. Following weight loss on either a high monounsaturated fat, standard protein (HMF; 50 % fat, 20 % protein (67 g/d), 30 % carbohydrate) or a high protein, moderate fat (HP) (40 % protein (136 g/d), 30 % fat, 30 % carbohydrate) energy-restricted diet (6000 kJ/d) subjects were asked to maintain the same dietary pattern without intensive dietary counselling for the following 36 weeks. Overall weight loss was 6.2 (SD 7.3) kg (P loss at the end of the study were sex, age and reported percentage energy from protein (R2 0.22, P loss and improvement in cardiovascular risk factors with no adverse effects of a high monounsaturated fat diet.

  16. Maintenance of nuclear power plants

    International Nuclear Information System (INIS)

    1982-01-01

    This Guide covers the organizational and procedural aspects of maintenance but does not give detailed technical advice on the maintenance of particular plant items. It gives guidance on preventive and remedial measures necessary to ensure that all structures, systems and components important to safety are capable of performing as intended. The Guide covers the organizational and administrative requirements for establishing and implementing preventive maintenance schedules, repairing defective plant items, providing maintenance facilities and equipment, procuring stores and spare parts, selecting and training maintenance personnel, reviewing and controlling plant modifications arising from maintenance, and for generating, collecting and retaining maintenance records. Maintenance shall be subject to quality assurance in all aspects important to safety. Because quality assurance has been dealt with in detail in other Safety Guides, it is only included here in specific instances where emphasis is required. Maintenance is considered to include functional and performance testing of plant, surveillance and in-service inspection, where these are necessary either to support other maintenance activities or to ensure continuing capability of structures, systems and components important to safety to perform their intended functions

  17. Maintenance Production Demand and Capacity Analysis of Third Maintenance Battalion

    Science.gov (United States)

    2017-12-01

    Delivery T/E Table of Equipment TFSMS Total Force Structure Management System TFSP Total Force Structure Process TM Technical Manual T/O Table of...exercises each year, both on Okinawa and throughout the Pacific theater. When equipment requires maintenance beyond the organizational -level...demand was analyzed based on customer cycle time (i.e., time that equipment was in Third Maintenance Battalion custody), maintenance cycle time during

  18. CANDU plant maintenance: Recent developments

    International Nuclear Information System (INIS)

    Charlebois, P.

    2000-01-01

    CANDU units have long been recognized for their exceptional safety and reliability. Continuing development in the maintenance area has played a key role in achieving this performance level. For over two decades, safety system availability has been monitored closely and system maintenance programs adjusted accordingly to maintain high levels of performance. But as the plants approach mid life in a more competitive environment and component aging becomes a concern, new methods and techniques are necessary. As a result, recent developments are moving the maintenance program largely from a corrective and preventive approach to predictive and condition based maintenance. The application of these techniques is also being extended to safety related systems. These recent developments include use of reliability centred methods to define system maintenance requirements and strategies. This approach has been implemented on a number of systems at Canadian CANDU plants with positive results. The pilot projects demonstrated that the overall maintenance effort remained relatively constant while the system performance improved. It was also possible to schedule some of the redundant component maintenance during plant operation without adverse impact on system availability. The probabilistic safety assessment was found to be useful in determining the safety implications of component outages. These new maintenance strategies are now making use of predictive and condition based maintenance techniques to anticipate equipment breakdown and schedule preventive maintenance as the need arises rather than time based. Some of these techniques include valve diagnostics, vibration monitoring, oil analysis, thermography. Of course, these tools and techniques must form part of an overall maintenance management system to ensure that maintenance becomes a living program. To facilitate this process and contain costs, new information technology tools are being introduced to provide system engineers

  19. Development of plant maintenance systems

    International Nuclear Information System (INIS)

    Tomita, Jinji; Ike, Masae; Nakayama, Kenji; Kato, Hisatomo

    1989-01-01

    Toshiba is making active efforts for the continuing improvement of reliability and maintainability of operating nuclear power plants. As a part of these efforts, the company has developed new maintenance administration systems, diagnostic monitoring facilities for plant equipments, computer-aided expert systems, and remote-controlled machines for maintenance work. The maintenance administration systems provide efficient work plans and data acquisition capabilities for the management of personnel and equipments involved in nuclear power plant maintenance. The plant diagnostic facilities monitor and diagnose plant conditions for preventive maintenance, as well as enabling rapid countermeasures to be carried out should they be required. Expert systems utilizing artificial intelligence (AI) technology are also employed. The newly developed remote-controlled machines are useful tools for the maintenance inspection of equipment which can not be easily accessed. (author)

  20. Towards Agile Ontology Maintenance

    Science.gov (United States)

    Luczak-Rösch, Markus

    Ontologies are an appropriate means to represent knowledge on the Web. Research on ontology engineering reached practices for an integrative lifecycle support. However, a broader success of ontologies in Web-based information systems remains unreached while the more lightweight semantic approaches are rather successful. We assume, paired with the emerging trend of services and microservices on the Web, new dynamic scenarios gain momentum in which a shared knowledge base is made available to several dynamically changing services with disparate requirements. Our work envisions a step towards such a dynamic scenario in which an ontology adapts to the requirements of the accessing services and applications as well as the user's needs in an agile way and reduces the experts' involvement in ontology maintenance processes.

  1. Cofrentes maintenance. Life essence

    International Nuclear Information System (INIS)

    Sala, J.

    2009-01-01

    Even though it may seem like something much more complex, maintenance is not more than the seed of continuity, the alchemy of eternal youth or the key to combating the cruel passage of time that haunts us all. Little does it matter where you are from or where you are going; it is irrelevant whether you are man, woman or machine. If you do not have a more or less sensible strategy of survival, reality can take you by surprise, without prior warning, at the wrong place and the wrong time. It is sad to see how what we thought was eternal, and thus do not care for and pamper as we should, eventually loses its qualities and functions over the years and fades away like a vague memory. (Author)

  2. A preventive maintenance lubricant

    Energy Technology Data Exchange (ETDEWEB)

    Chapaykina, S A; Ol' kov, P L; Pertsev, A N; Rodzevillo, I T; Rogacheva, O I; Zinov' yev, A P

    1980-02-15

    A method is disclosed to lower the adherence of materials by reducing the viscosity of a preventive maintenance lubricant against adhesion of moist, freeflowing materials, containing extract of selective cleaning of oil fractions and asphalt. Gas oil of catalytic cracking is added having a boiling point of 190-300 degrees, with the following ratio of components (%): selective cleaning extract, 43-50; asphalt, 14-16; and gas oil of catalytic cracking, the remainder, Treating a surface with the proposed lubricant lowers the specific resistance to shift of rock compared with the prototype (g/cm/sup 2/): sand (moisture content, 18%) from 3.82 to 1.55; and clay (moisture content 16%), from 5.41 to 3.51.

  3. Strategy to maximize maintenance operation

    OpenAIRE

    Espinoza, Michael

    2005-01-01

    This project presents a strategic analysis to maximize maintenance operations in Alcan Kitimat Works in British Columbia. The project studies the role of maintenance in improving its overall maintenance performance. It provides strategic alternatives and specific recommendations addressing Kitimat Works key strategic issues and problems. A comprehensive industry and competitive analysis identifies the industry structure and its competitive forces. In the mature aluminium industry, the bargain...

  4. Buildings Lean Maintenance Implementation Model

    Science.gov (United States)

    Abreu, Antonio; Calado, João; Requeijo, José

    2016-11-01

    Nowadays, companies in global markets have to achieve high levels of performance and competitiveness to stay "alive".Within this assumption, the building maintenance cannot be done in a casual and improvised way due to the costs related. Starting with some discussion about lean management and building maintenance, this paper introduces a model to support the Lean Building Maintenance (LBM) approach. Finally based on a real case study from a Portuguese company, the benefits, challenges and difficulties are presented and discussed.

  5. Maintenance technologies for reactor internals

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Kenji [Nuclear Energy Systems and Services Div., Toshiba Corp., Tokyo (Japan); Kobayashi, Masahiro [Toshiba Corp., Yokohama (Japan). Keihin Product Operations; Sano, Yuji; Kimura, Seiichiro [Power and Industrial Systems Research and Development Center, Toshiba Corp., Tokyo(Japan)

    2000-10-01

    Toshiba places the highest priority on maintenance technologies for the reactor pressure vessel (RPV) and its internals in operating nuclear power plants. This paper summarizes the status of applied laser maintenance technologies, both preventive and repair. For laser peeing and laser desensitization treatment (LDT) technologies in particular, field applications are also described in detail. In the future, the area of field applications for preventive maintenance, repair, and inspection technologies will be further expanded. (author)

  6. Maintenance management for nuclear power plant 'Integrated valve maintenance'

    International Nuclear Information System (INIS)

    Gerner, P.; Zanner, G.

    2001-01-01

    The deregulation of Europe's power market does force many utilities, and especially nuclear power plant operators, to introduce extensive cost-cutting measures in order to be able to compete within this new environment. The optimization of plant outages provides considerable potential for raising plant availability but can also lower operating costs by reducing e.g. expenditure on maintenance. Siemens Nuclear Power GmbH, in cooperation with plant operators, is currently implementing new and improved service concepts which can have a major effect on the way in which maintenance will be performed in the future. Innovative service packages for maintenance in nuclear power plants are available which can be used to perform a time- and cost-effective maintenance. The concepts encompass optimization of the overall sequence from planning in advance to the individual measures including reduction of the scope of maintenance activities, identification of cost cutting potential and bundling of maintenance activities. The main features of these maintenance activities are illustrated here using the examples of outage planning and integrated valve maintenance. In nuclear power plants approx. 5000 valves are periodically preventively, condition-based or breakdown-based maintained. Because of this large number of valves to be maintained a high potential of improvements and cost reductions can be achieved by performing an optimized, cost-effective maintenance based on innovative methods and tools. Siemens Nuclear Power GmbH has developed and qualified such tools which allow to reduce service costs while maintaining high standards of safety and availability. By changing from preventive to predictive (condition-based) maintenance - the number of valves to be maintained may be reduced considerably. The predictive maintenance is based on the Siemens Nuclear Power GmbH diagnostic and evaluation method (ADAM). ADAM is used to monitor the operability of valves by analytical verification of

  7. Transformer oil maintenance

    Energy Technology Data Exchange (ETDEWEB)

    White, J. [A.F. White Ltd., Brantford, ON (Canada)

    2002-08-01

    Proactive treatment is required in the case of transformer oil, since the oil degrades over time, which could result in the potential failure of the transformer or costly repairs. A mineral-based oil is used for transformers because of its chemical properties and dielectric strength. Water and particulate are the main contaminants found in transformer oil, affecting the quality of the oil through reduced insulation. Acid that forms in the oil when reacting with oxygen is called oxidization. It reduces the heat dissipation of the transformer as the acid forms sludge which settles on the windings of the transformer. The first step in the preventive maintenance program associated with transformer oil is the testing of the oil. The base line is established through initial testing, and subsequent annual testing identifies any changes. The minimal requirements are: (1) dielectric breakdown, a measure of the voltage conducted by the oil; (2) neutralization/acid number, which detects the level of acid present in the oil; (3) interfacial tension, which identifies the presence of polar compounds; (4) colour, which displays quality, aging and the presence of contaminants; and (5) water, which decreases the dielectric breakdown voltage. The analysis of the gases present in the oil is another useful tool in a maintenance program for the determination of a possible fault such as arcing, corona or overheated connections and is accomplished through Dissolved Gas Analysis (DGA). Remediation treatment includes upgrading the oil. Ideally, reclamation should be performed in the early stages of the acid buildup before sludging occurs. Onsite reclamation includes Fuller's earth processing and degasification, a process briefly described by the author.

  8. Nuclear maintenance and management system

    International Nuclear Information System (INIS)

    Yamaji, Yoshihiro; Abe, Norihiko

    2000-01-01

    The Mitsubishi Electric Co., Ltd. has developed to introduce various computer systems for desk-top business assistance in a power plant such as system isolation assisting system, operation parameter management system, and so on under aiming at business effectiveness since these ten and some years. Recently, by further elapsed years of the plants when required for further cost reduction and together with change of business environment represented by preparation of individual personal computer, further effectiveness, preparation of the business environment, and upgrading of maintenance in power plant business have been required. Among such background, she has carried out various proposals and developments on construction of a maintenance and management system integrated the business assistant know-hows and the plant know-hows both accumulated previously. They are composed of three main points on rationalization of business management and document management in the further effectiveness, preparation of business environment, TBM maintenance, introduction of CBM maintenance and introduction of maintenance assistance in upgrading of maintenance. Here was introduced on system concepts aiming at the further effectiveness of the nuclear power plant business, preparation of business environment, upgrading of maintenance and maintenance, and so on, at a background of environment around maintenance business in the nuclear power plants (cost-down, highly elapsed year of the plant, change of business environment). (G.K)

  9. Remote maintenance development for ITER

    International Nuclear Information System (INIS)

    Tada, Eisuke; Shibanuma, Kiyoshi

    1998-01-01

    This paper describes the overall ITER remote maintenance design concept developed mainly for in-vessel components such as diverters and blankets, and outlines the ITER R and D program to develop remote handling equipment and radiation hard components. Reactor structures inside the ITER cryostat must be maintained remotely due to DT operation, making remote handling technology basic to reactor design. The overall maintenance scenario and design concepts have been developed, and maintenance design feasibility, including fabrication and testing of full-scale in-vessel remote maintenance handling equipment and tool, is being verified. (author)

  10. Remote maintenance development for ITER

    Energy Technology Data Exchange (ETDEWEB)

    Tada, Eisuke [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Shibanuma, Kiyoshi

    1998-04-01

    This paper describes the overall ITER remote maintenance design concept developed mainly for in-vessel components such as diverters and blankets, and outlines the ITER R and D program to develop remote handling equipment and radiation hard components. Reactor structures inside the ITER cryostat must be maintained remotely due to DT operation, making remote handling technology basic to reactor design. The overall maintenance scenario and design concepts have been developed, and maintenance design feasibility, including fabrication and testing of full-scale in-vessel remote maintenance handling equipment and tool, is being verified. (author)

  11. Maintenance performance improvement with System Dynamics : A Corrective Maintenance showcase

    NARCIS (Netherlands)

    Deenen, R.E.M.; Van Daalen, C.E.; Koene, E.G.C.

    2008-01-01

    This paper presents a case study of an analysis of a Corrective Maintenance process to realize performance improvement. The Corrective Maintenance process is supported by SAP, which has indicated the performance realisation problem. System Dynamics is used in a Group Model Building process to

  12. Condition based maintenance in the context of opportunistic maintenance

    NARCIS (Netherlands)

    Koochaki, Javid; Bokhorst, Jos A. C.; Wortmann, Hans; Klingenberg, Warse

    2012-01-01

    Condition based maintenance (CBM) uses the operating condition of a component to predict a failure event. Compared to age based replacement (ABR), CBM usually results in higher availability and lower maintenance costs, since it tries to prevent unplanned downtime and avoid unnecessary preventive

  13. A STEPPING STONE TOWARDS KNOWLEDGE BASED MAINTENANCE

    OpenAIRE

    G. Waeyenbergh; L. Pintelon; L. Gelders

    2012-01-01

    Maintenance decision making becomes more and more a management concern. Some decades ago, maintenance was still often considered as an unavoidable side effect of production. The perception of maintenance has evolved considerably. One of the current issues is the maintenance concept, being the mix of maintenance interventions and the general framework for determining this mix. In this paper we describe a modular framework, called Knowledge Based Maintenance, for developing a customised mainten...

  14. Residual life management. Maintenance improvement

    International Nuclear Information System (INIS)

    Sainero Garcia, J.; Hevia Ruperez, F.

    1995-01-01

    The terms Residual Life Management, Life Cycle Management and Long-Term Management are synonymous with a concept which aims to establish efficient maintenance for the profitable and safe operation of a power plant for as long as possible. A Residual Life Management programme comprises a number of stages, of which Maintenance Evaluation focuses on how power plant maintenance practices allow the mitigation and control of component ageing. with this objective in mind, a methodology has been developed for the analysis of potential degradative phenomena acting on critical components in terms of normal power plant maintenance practices. This methodology applied to maintenance evaluation enables the setting out of a maintenance programme based on the Life Management concept, and the programme's subsequent up-dating to allow for new techniques and methods. Initial applications have shown that although, in general terms, power plant maintenance is efficient, the way in which Residual Life Management is approached requires changes in maintenance practices. These changes range from modifications to existing inspection and surveillance methods or the establishment of new ones, to the monitoring of trends or the performance of additional studies, the purpose of which is to provide an accurate evaluation of the condition of the installations and the possibility of life extension. (Author)

  15. The Nordic maintenance care program

    DEFF Research Database (Denmark)

    Myburgh, Corrie; Brandborg-Olsen, Dorthe; Albert, Hanne

    2013-01-01

    To describe and interpret Danish Chiropractors' perspectives regarding the purpose and rationale for using MC (maintenance care), its content, course and patient characteristics.......To describe and interpret Danish Chiropractors' perspectives regarding the purpose and rationale for using MC (maintenance care), its content, course and patient characteristics....

  16. Achieving world class maintenance status

    Energy Technology Data Exchange (ETDEWEB)

    Tomlingson, P.D. [Paul D. Tomingson Associates (United States)

    2007-08-15

    The article written by a management consultant, discusses the art of successful planning and operation of maintenance in mines considering factors such as benchmaking, key performance indices (KPIs) and frequency of procedures which can help achieve 'world class maintenance'. 1 fig.

  17. Recent Research on Language Maintenance.

    Science.gov (United States)

    Garcia, MaryEllen

    2003-01-01

    Describes recent research on language maintenance to provide broad, worldwide coverage of different language contact situations. Surveys various countries in which research within ethnic and minority language communities illuminates language maintenance or shift, or revitalization, for that group. (Author/VWL)

  18. Maintenance Planning for Historic Buildings

    Directory of Open Access Journals (Sweden)

    Diana Plian

    2008-01-01

    Full Text Available The key to good maintenance of historic buildings is a long-range maintenance plan. Long-range planning recognizes a responsibility to the future to prolong the useful life of a building by preserving it in its present condition and preventing or slowing deterioration and damage from natural or other causes.

  19. Ribosomal DNA-binding proteins in the nucleolus of Physarum polycephalum

    International Nuclear Information System (INIS)

    Graham-Lorence, S.E.

    1987-01-01

    In Physarum polycephalum, the nucleoli are extra chromosomal structures containing 200 to 400 copies of a linear 60 kilobase palindromic rDNA molecule. These rDNA molecules are organized into minichromosomes which apparently are held within a nucleolar protein matrix. To obtained evidence for attachment of the rDNA to such a matrix, both intact and lithium diiodosalicylate/NaCl-extracted nucleoli were digested for various lengths of time with micrococcal nuclease, so that portions of the rDNA molecules not attached within the nucleolar structure would be released. Nucleolar DNA-binding proteins were determined by blotting electrophoretically separated proteins from SDS-polyacrylamide gels onto nitrocellulose paper and probing them with radiolabeled DNA. In addition to the histones and lexosome proteins, eight DNA-binding proteins were identified having molecular weights of 25, 38, 47, 53, 55, 67, and 70 kD, with the 47, 53, 67, and 70 kD proteins requiring Ca 2+ for binding

  20. Space Telescope maintenance and refurbishment

    Science.gov (United States)

    Trucks, H. F.

    1983-01-01

    The Space Telescope (ST) represents a new concept regarding spaceborne astronomical observatories. Maintenance crews will be brought to the orbital worksite to make repairs and replace scientific instruments. For major overhauls the telescope can be temporarily returned to earth with the aid of the Shuttle. It will, thus, be possible to conduct astronomical studies with the ST for two decades or more. The five first-generation scientific instruments used with the ST include a wide field/planetary camera, a faint object camera, a faint object spectrograph, a high resolution spectrograph, and a high speed photometer. Attention is given to the optical telescope assembly, the support systems module, aspects of mission and science operations, unscheduled maintenance, contingency orbital maintenance, planned on-orbit maintenance, ground maintenance, ground refurbishment, and ground logistics.

  1. Remote maintenance development for ITER

    International Nuclear Information System (INIS)

    Tada, Eisuke; Shibanuma, Kiyoshi

    1997-01-01

    This paper both describes the overall design concept of the ITER remote maintenance system, which has been developed mainly for use with in-vessel components such as divertor and blanket, and outlines of the ITER R and D program, which has been established to develop remote handling equipment/tools and radiation hard components. In ITER, the reactor structures inside cryostat have to be maintained remotely because of activation due to DT operation. Therefore, remote-handling technology is fundamental, and the reactor-structure design must be made consistent with remote maintainability. The overall maintenance scenario and design concepts of the required remote handling equipment/tools have been developed according to their maintenance classification. Technologies are also being developed to verify the feasibility of the maintenance design and include fabrication and testing of a fullscale remote-handling equipment/tools for in-vessel maintenance. (author)

  2. Improving the TRIGA facility maintenance by predictive maintenance techniques

    International Nuclear Information System (INIS)

    Preda, M.; Sabau, C.; Barbalata, E.

    1997-01-01

    This work deals with the specific operation of equipment in radioactive environment or in conditions allowing radioactive contamination. The requirements of remote operation ensuring the operators' protection are presented. Also, the requirements of international standards issued by IAEA-Vienna are reviewed. The organizational withdraws of the maintenance activities, based on the standards and maintenance and repair directives still in force, are shown. It is emphasized the fact that this type of maintenance was adequate to a given level of technical development, characteristic for pre-computerized industry, but, at present, it is obsolete and uneconomic both in utilization and maintenance. Such a system constitutes already a burden hindering the efforts of maximizing the availability, maintenance, prolongation the service life of equipment and utilities, finally, of increasing the efficiency of complex installations. Moreover, the predictive maintenance techniques are strongly requested by the character of radioactive installations precluding the direct access in given zones (a potential risk of irradiation or radioactive contamination) of installations during operation. The results obtained by applying the predictive maintenance techniques in the operation of the double circuit irradiation loop, used in the TRIGA reactors, are presented

  3. Enhancing Nanos expression via the bacterial TomO protein is a conserved strategy used by the symbiont Wolbachia to fuel germ stem cell maintenance in infected Drosophila females.

    Science.gov (United States)

    Ote, Manabu; Yamamoto, Daisuke

    2018-04-27

    The toxic manipulator of oogenesis (TomO) protein has been identified in the wMel strain of Wolbachia that symbioses with the vinegar fly Drosophila melanogaster, as a protein that affects host reproduction. TomO protects germ stem cells (GSCs) from degeneration, which otherwise occurs in ovaries of host females that are mutant for the gene Sex-lethal (Sxl). We isolated the TomO homologs from wPip, a Wolbachia strain from the mosquito Culex quinquefasciatus. One of the homologs, TomO w Pip 1, exerted the GSC rescue activity in fly Sxl mutants when lacking its hydrophobic stretches. The GSC-rescuing action of the TomO w Pip 1 variant was ascribable to its abilities to associate with Nanos (nos) mRNA and to enhance Nos protein expression. The analysis of structure-activity relationships with TomO homologs and TomO deletion variants revealed distinct modules in the protein that are each dedicated to different functions, i.e., subcellular localization, nos mRNA binding or Nos expression enhancement. We propose that modular reshuffling is the basis for structural and functional diversification of TomO protein members. © 2018 Wiley Periodicals, Inc.

  4. Assessing maintenance contracts when preventive maintenance is outsourced

    International Nuclear Information System (INIS)

    Wu Shaomin

    2012-01-01

    In some companies, corrective maintenance is conducted in-house but preventive maintenance might be outsourced. This raises a need to optimise some parameters such as the number of contracts from a perspective of the equipment owner. This paper considers a maintenance policy for such a situation, analyses the roles of the parameters in a PM model, proposes approaches to defining bonus functions, and finally discusses special cases of both the PM policy and the bonus function. Numerical examples are also given to explore the impact of parameters on the expected lifecycle cost rate.

  5. Maintenance training centre at NPP Paks, Hungary

    International Nuclear Information System (INIS)

    Babos, K.

    1996-01-01

    The lecture shows the feature of WWER-440/213 units maintenance, the existing maintenance training system, the necessity of the change in maintenance training system at NPP Paks. The author introduces the would-be maintenance training centre, the training facilities and the main tasks related to the maintenance training. (author)

  6. 340 Facility maintenance implementation plan

    International Nuclear Information System (INIS)

    1995-03-01

    This Maintenance Implementation Plan (MIP) has been developed for maintenance functions associated with the 340 Facility. This plan is developed from the guidelines presented by Department of Energy (DOE) Order 4330.4B, Maintenance Management Program (DOE 1994), Chapter II. The objective of this plan is to provide baseline information for establishing and identifying Westinghouse Hanford Company (WHC) conformance programs and policies applicable to implementation of DOE order 4330.4B guidelines. In addition, this maintenance plan identifies the actions necessary to develop a cost-effective and efficient maintenance program at the 340 Facility. Primary responsibility for the performance and oversight of maintenance activities at the 340 Facility resides with Westinghouse Hanford Company (WHC). Maintenance at the 340 Facility is performed by ICF-Kaiser Hanford (ICF-KH) South Programmatic Services crafts persons. This 340 Facility MIP provides interface requirements and responsibilities as they apply specifically to the 340 Facility. This document provides an implementation schedule which has been developed for items considered to be deficient or in need of improvement. The discussion sections, as applied to implementation at the 340 Facility, have been developed from a review of programs and practices utilizing the graded approach. Biennial review and additional reviews are conducted as significant programmatic and mission changes are made. This document is revised as necessary to maintain compliance with DOE requirements

  7. 340 Facility maintenance implementation plan

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    This Maintenance Implementation Plan (MIP) has been developed for maintenance functions associated with the 340 Facility. This plan is developed from the guidelines presented by Department of Energy (DOE) Order 4330.4B, Maintenance Management Program (DOE 1994), Chapter II. The objective of this plan is to provide baseline information for establishing and identifying Westinghouse Hanford Company (WHC) conformance programs and policies applicable to implementation of DOE order 4330.4B guidelines. In addition, this maintenance plan identifies the actions necessary to develop a cost-effective and efficient maintenance program at the 340 Facility. Primary responsibility for the performance and oversight of maintenance activities at the 340 Facility resides with Westinghouse Hanford Company (WHC). Maintenance at the 340 Facility is performed by ICF-Kaiser Hanford (ICF-KH) South Programmatic Services crafts persons. This 340 Facility MIP provides interface requirements and responsibilities as they apply specifically to the 340 Facility. This document provides an implementation schedule which has been developed for items considered to be deficient or in need of improvement. The discussion sections, as applied to implementation at the 340 Facility, have been developed from a review of programs and practices utilizing the graded approach. Biennial review and additional reviews are conducted as significant programmatic and mission changes are made. This document is revised as necessary to maintain compliance with DOE requirements.

  8. Developing optimized prioritizing road maintenance

    Directory of Open Access Journals (Sweden)

    Ewadh Hussein Ali

    2018-01-01

    Full Text Available Increased demand for efficient maintenance of the existing roadway system needs optimal usage of the allocated funds. The paper demonstrates optimized methods for prioritizing maintenance implementation projects. A selected zone of roadway system in Kerbala city represents the study area to demonstrate the application of the developed prioritization process. Paver system PAVER integrated with GIS is used to estimate and display the pavement condition index PCI, thereby to establish a priority of maintenance. In addition to simple ranking method by PCI produced by the output of PAVER, the paper introduces PCI measure for each section of roadway. The paper introduces ranking by multiple measures investigated through expert knowledge about measures that affect prioritization and their irrespective weights due to a predesigned questionnaire. The maintenance priority index (MPI is related to cost of suitable proposed maintenance, easiness of proposed maintenance, average daily traffic and functional classification of the roadway in addition to PCI. Further, incremental benefit-cost analysis ranking provide an optimized process due to benefit and cost of maintenance. The paper introduces efficient display of layout and ranking for the selected zone of roadway system based on MPI index and incremental BCR method. Although the two developed methods introduce different layout display for priority, statistical test shows that no significant difference between ranking of all methods of prioritization.

  9. TECHNICAL MAINTENANCE EFFICIENCY OF THE AIRCRAFT MAINTENANCE-FREE ON-BOARD SYSTEM BETWEEN SCHEDULED MAINTENANCES

    Directory of Open Access Journals (Sweden)

    A. M. Bronnikov

    2017-01-01

    Full Text Available The avionics concept of the maintenance-free on-board equipment implies the absence of necessity to maintain onboard systems between scheduled maintenance, preserving the required operational and technical characteristics; it should be achieved by automatic diagnosis of the technical condition and the application of active means of ensuring a failsafe design, allowing to change the structure of the system to maintain its functions in case of failure. It is supposed that such equipment will reduce substantially and in the limit eliminate traditional maintenance of aircraft between scheduled maintenance, ensuring maximum readiness for use, along with improving safety. The paper proposes a methodology for evaluating the efficiency of maintenance-free between scheduled maintenance aircraft system with homogeneous redundancy. The excessive redundant elements allow the system to accumulate failures which are repaired during the routine maintenance. If the number of failures of any reserve is approaching a critical value, the recovery of the on-board system (elimination of all failures is carried out between scheduled maintenance by conducting rescue and recovery operations. It is believed that service work leads to the elimination of all failures and completely updates the on-board system. The process of system operational status changes is described with the discrete-continuous model in the flight time. The average losses in the sorties and the average cost of operation are used as integrated efficiency indicators of system operation. For example, the evaluation of the operation efficiency of formalized on-board system with homogeneous redundancy demonstrates the efficiency of the proposed methodology and the possibility of its use while analyzing the efficiency of the maintenance-free operation equipment between scheduled periods. As well as a comparative analysis of maintenance-free operation efficiency of the on-board system with excessive

  10. Maintenance Energy Requirements of Double-Muscled Belgian Blue Beef Cows

    OpenAIRE

    Fiems, Leo O.; De Boever, Johan L.; Vanacker, José M.; De Campeneere, Sam

    2015-01-01

    Simple Summary Double-muscled Belgian Blue animals are extremely lean, characterized by a deviant muscle fiber type with more fast-glycolytic fibers, compared to non-double-muscled animals. This fiber type may result in lower maintenance energy requirements. On the other hand, lean meat animals mostly have a higher rate of protein turnover, which requires more energy for maintenance. Therefore, maintenance requirements of Belgian Blue cows were investigated based on a zero body weight gain. T...

  11. Depot Maintenance: Executed Workload and Maintenance Operations at DOD Depots

    Science.gov (United States)

    2017-02-03

    in turn enable testing of helicopter rotor blades. The Army has also made investments in logistics software at all of its depots. According to the...maintenance and repair of structural helicopter airframes and blades; advanced composite technologies; flight controls and control surfaces; and...level maintenance on a wide range of vehicles and other military assets, including helicopters , combat vehicles, ships, aircraft, engines, and software

  12. Remote handling maintenance of ITER

    International Nuclear Information System (INIS)

    Haange, R.

    1999-01-01

    The remote maintenance strategy and the associated component design of the International Thermonuclear Experimental Reactor (ITER) have reached a high degree of completeness, especially with respect to those components that are expected to require frequent or occasional remote maintenance. Large-scale test stands, to demonstrate the principle feasibility of the remote maintenance procedures and to develop the required equipment and tools, were operational at the end of the Engineering Design Activities (EDA) phase. The initial results are highly encouraging: major remote equipment deployment and component replacement operations have been successfully demonstrated. (author)

  13. Maintenance of radiation monitoring systems

    International Nuclear Information System (INIS)

    Aoyama, Kei

    2001-01-01

    As the safety and quality of atomic power facilities are more strongly required, the reliability improvement and preventive maintenance of radiation monitoring systems are important. This paper describes the maintenance of radiation monitoring systems delivered by Fuji Electric and the present status of preventive maintenance technology. Also it introduces the case that we developed a fault diagnosis function adopting a statistics technique and artificial intelligence (AI) and delivered a radiation monitoring system including this function. This system can output a fault analysis result and a countermeasure from the computer in real time. (author)

  14. Overview of maintenance principles and regulatory supervision of maintenance activities at nuclear power plants in Slovakia

    International Nuclear Information System (INIS)

    Rohar, S.; Cepcek, S.

    1997-01-01

    The maintenance represents one of the most important tools to ensure safe and reliable operation of nuclear power plants. The emphasis of Nuclear Regulatory Authority of the Slovak Republic to the maintenance issue is expressed by requirements in the regulations. The current practice of maintenance management in operated nuclear power plants in Slovak Republic is presented. Main aspects of maintenance, as maintenance programme, organization of maintenance, responsibilities for maintenance are described. Activities of nuclear regulatory authority in maintenance process are presented too. (author)

  15. Contract Service for School Maintenance

    Science.gov (United States)

    Modern Schools, 1976

    1976-01-01

    Preventive maintenance can extend useful equipment life in a school building and keep systems running more efficiently. Points to consider before selecting a comprehensive energy management package are listed. (Author/MLF)

  16. Managing Custodial and Maintenance Staffs.

    Science.gov (United States)

    Fickes, Michael

    2001-01-01

    Presents some basic maintenance management techniques that can help schools meet their budgets, preserve staffing levels, meet productivity needs, and sustain quality services. Tips for staff recruitment, training, and retention are explored. (GR)

  17. Health Maintenance Organization (HMO) Plan

    Science.gov (United States)

    ... Find & compare doctors, hospitals, & other providers Health Maintenance Organization (HMO) Plan In most HMO Plans, you generally ... certain service when needed. Related Resources Preferred Provider Organization (PPO) Private Fee-for-Service (PFFS) Special Needs ...

  18. Genetic variation and its maintenance

    International Nuclear Information System (INIS)

    Roberts, D.F.; De Stefano, G.F.

    1986-01-01

    This book contains several papers divided among three sections. The section titles are: Genetic Diversity--Its Dimensions; Genetic Diversity--Its Origin and Maintenance; and Genetic Diversity--Applications and Problems of Complex Characters

  19. Functional update of the auxiliary proteins PsbW, PsbY, HCF136, PsbN, TerC and ALB3 in maintenance and assembly of PSII

    Directory of Open Access Journals (Sweden)

    Magdalena ePlöchinger

    2016-04-01

    Full Text Available Assembly of Photosystem (PS II in plants has turned out to be a highly complex process which, at least in part, occurs in a sequential order and requires many more auxiliary proteins than subunits present in the complex. Owing to the high evolutionary conservation of the subunit composition and the three-dimensional structure of the PSII complex, most plant factors involved in the biogenesis of PSII originated from cyanobacteria and only rarely evolved de novo. Furthermore, in chloroplasts the initial assembly steps occur in the non-appressed stroma lamellae, whereas the final assembly including the attachment of the major LHCII antenna proteins takes place in the grana regions. The stroma lamellae are also the place where part of PSII repair occurs, which very likely also involves assembly factors. In cyanobacteria initial PSII assembly also occurs in the thylakoid membrane, in so-called thylakoid centres, which are in contact with the plasma membrane. Here, we provide an update on the structures, localisations, topologies, functions, expression and interactions of the low molecular mass PSII subunits PsbY, PsbW and the auxiliary factors HCF136, PsbN, TerC and ALB3, assisting in PSII complex assembly and protein insertion into the thylakoid membrane.

  20. The ITER remote maintenance system

    International Nuclear Information System (INIS)

    Tesini, A.; Palmer, J.

    2008-01-01

    The aim of this paper is to summarize the ITER approach to machine components maintenance. A major objective of the ITER project is to demonstrate that a future power producing fusion device can be maintained effectively and offer practical levels of plant availability. During its operational lifetime, many systems of the ITER machine will require maintenance and modification; this can be achieved using remote handling methods. The need for timely, safe and effective remote operations on a machine as complex as ITER and within one of the world's most hostile remote handling environments represents a major challenge at every level of the ITER Project organization, engineering and technology. The basic principles of fusion reactor maintenance are presented. An updated description of the ITER remote maintenance system is provided. This includes the maintenance equipment used inside the vacuum vessel, inside the hot cell and the hot cell itself. The correlation between the functions of the remote handling equipment, of the hot cell and of the radwaste processing system is also described. The paper concludes that ITER has equipped itself with a good platform to tackle the challenges presented by its own maintenance and upgrade needs

  1. Maintenance of nuclear power plants

    International Nuclear Information System (INIS)

    Migaud, D.; Hutin, J.P.; Jouette, I.; Eymond, P.; Devie, P.; Cudelou, C.; Magnier, S.; Frydman, M.

    2016-01-01

    This document gathers different articles concerning the maintenance of the French nuclear power plants. The first article analyses the impact of the recent law on the energetic transition that sets the share of nuclear power at 50% of the electricity produced by 2025. A consequence may be the decommissioning of 17 to 20 reactors by 2025 and the huge maintenance program called 'Grand Carenage' whose aim is to extend operating life over 40 years will have to be re-considered in order to avoid useless expenses. The second article shows that in 2015 the French nuclear reactor fleet got very good results in terms of availability and safety. There were 49 scheduled outages and among them some ended ahead of time. The third article describes the specificities of the maintenance of a nuclear power plant, for instance the redundancy of some systems implies that maintenance has to deal with systems that have never functioned but must be ready to operate at any moment. Another specificity is the complexity of a nuclear power plant that implies an essential phase of preparation for maintenance operations. Because of safety requirements any maintenance operation has to be controlled, checked and may provide feedback. The fourth article presents the 'Grand Carenage' maintenance program that involves the following operations: the replacement of steam generators, the re-tubing of condensers, the replacement of the filtering drums used for cooling water, the testing of the reactor building, the hydraulic test of the primary circuit and the inspection of the reactor vessel. The fifth article focuses on the organization of the work-site for maintenance operations and the example of the Belleville-sur-Loire is described in the sixth article. Important maintenance operations like 'Grand Carenage' requires a strong collaboration with a network of specialized enterprises and as no reactor (except Flamanville EPR) is being built in France, maintenance

  2. Maintenance proficiency evaluation test bank

    International Nuclear Information System (INIS)

    Maier, Loran

    2003-01-01

    The Maintenance Proficiency Evaluation Test Bank (MPETB) is an Electric Power Research Institute- (EPRJ-) operated, utility-sponsored means of developing, maintaining, and disseminating secure, high-quality written and performance maintenance proficiency tests. EPRTs charter is to ensure that all tests and test items that go into the Test Bank have been validated, screened for reliability, and evaluated to high standards of psychometric excellence. Proficiency tests of maintenance personnel.(mechanics, electricians, and instrumentation and control [I and C] technicians) are most often used to determine if an experienced employee is capable of performing maintenance tasks without further training. Such tests provide objective evidence for decisions to exempt an employee from what, for the employee, is unnecessary training. This leads to considerable savings in training costs and increased productivity because supervisors can assign personnel to tasks at which their competence is proven. The ultimate objective of proficiency evaluation is to ensure that qualified maintenance personnel are available to meet the maintenance requirements of the plant Numerous task-specific MPE tests (both written and performance) have been developed and validated using the EPRI MPE methodology by the utilities participating in the MPETB project A task-specific MPE consists of a multiple-choice written examination and a multi-step performance evaluation that can be used to assess an individual's present knowledge and skill level for a given maintenance task. The MPETB contains MPEs and test items for the mechanical, electrical, and I and C classifications that are readily available to participating utilities. Presently, utilities are placing emphasis on developing MPEs to evaluate outage-related maintenance tasks that demonstrate the competency and qualifications of plant and contractor personnel before the start of outage work. Utilities are also using the MPE methodology and process to

  3. Maintenance analysis method and operational feedback: a comprehensive maintenance management

    International Nuclear Information System (INIS)

    Mathieu Riou; Victor Planchon

    2006-01-01

    Full text of publication follows: Current periodic inspections program carried out on the COGEMA LOGISTICS casks is required by regulations and approved by the competent Authority. Thus, Safety and casks conformity to the according certificate of approval are guaranteed. Nonetheless, based on experience it appeared that some maintenance operations did not seem relevant or were redundant. Then, it was decided to rethink completely our maintenance program to reach the following objectives: - Set up the 'a minima' required inspection operations required to guarantee Safety and conformity to the certificate of approval, - Optimize criteria and periodicities of inspections taking into account: operational feedback, routine inspections carried out for each transport, regulations, environmental impact (ALARA, waste reduction,...), cost-effectiveness (reduction of cask's immobilization period,...). - Set up a maintenance program in Safety Analysis Reports that: stands alone (no need to check the specification or the certificate of approval to have the complete list of inspections mandatory to guarantee Safety), gives objectives instead of means of controls. This approach needs then to be re-evaluated by the competent Authority. Study's scope has been limited to the TN TM 12 cask family which is intensely used. COGEMA LOGISTICS has a high operational feedback on these casks. After Authority agreement, and in accordance with its requirements, study will then be extended to the other casks belonging to the COGEMA LOGISTICS cask fleet. Actually, the term 'maintenance' is linked to 'Base maintenance' and 'Main maintenance' and implicitly means that the cask is immobilized for a given period. To emphasize the modifications, the term 'maintenance' is no longer used and is substituted by 'periodic upkeep'. By changing the name, COGEMA LOGISTICS wants to emphasize that: some operations can for instance be realized while the cask is unloaded, periodicities are thought in terms of

  4. Knowledge engineering thinking of maintenance

    International Nuclear Information System (INIS)

    2007-01-01

    Maintenance optimization problem could not always settled mathematically and was obliged to use quasi-optimum solution with omitting non-formulated limiting condition or neglecting part of optimization object. In such a case knowledge engineering thinking was encouraged. Maintenance of complicated plant and artificial system should be considered from artificial object (equipment/facility hardware and system), technical information and knowledge base, and organizational and human aspect or society and institution. Comprehensive management system in organization and society was necessary not only for assuring integrity of equipment but also for attaining higher performance, reliability and economics of system. For better judgment it was important to share mechanism to make use of more information with organization or whole society. It was required to create database and data mining for knowledge base management system of maintenance. Maintenance was called 'last fortress' to assure quality such as reliability and safety of required function of equipment. Strategic approach to develop maintenance technology under cooperation was considered. Life extension R and D road map was launched in 2005. (T. Tanaka)

  5. TPX remote maintenance and shielding

    International Nuclear Information System (INIS)

    Rennich, M.J.; Nelson, B.E.

    1994-01-01

    The Tokamak Physics Experiment machine design incorporates comprehensive planning for efficient and safe component maintenance. Three programmatic decisions have been made to insure the successful implementation of this objective. First, the tokamak incorporates radiation shielding to reduce activation of components and limit the dose rate to personnel working on the outside of the machine. This allows most of the ex-vessel equipment to be maintained through conventional ''hands-on'' procedures. Second, to the maximum extent possible, low activation materials will be used inside the shielding volume. This resulted in the selection of Titanium (Ti-6Al-4V) for the vacuum vessel and PFC structures. The third decision stipulated that the primary in-vessel components will be replaced or repaired via remote maintenance tools specifically provided for the task. The component designers have been given the responsibility of incorporating maintenance design and for proving the maintainability of the design concepts in full-scale mockup tests prior to the initiation of final fabrication. Remote maintenance of the TPX machine is facilitated by general purpose tools provided by a special purpose design team. Major tools will include an in-vessel transporter, a vessel transfer system and a large component transfer container. In addition, tools such as manipulators and remotely operable impact wrenches will be made available to the component designers by this group. Maintenance systems will also provide the necessary controls for this equipment

  6. Experience based reliability centered maintenance

    International Nuclear Information System (INIS)

    Haenninen, S.; Laakso, K.

    1993-03-01

    The systematic analysis and documentation of operating experiences should be included in a living NPP life management program. Failure mode and effects and maintenance effects analyses are suitable methods for analysis of the failure and corrective maintenance experiences of equipment. Combined use of the information on occurred functional failures and the decision tree logic of the reliability centered maintenance identifies applicable and effective preventive maintenance tasks of equipment in an old plant. In this study the electrical motor drives of closing and isolation valves (MOV) of TVO and Loviisa nuclear power plants were selected to serve as pilot study objects. The study was limited to valve drives having actuators manufactured by AUMA in Germany. The fault and maintenance history of MOVs from 1981 up to and including October 1991 in different safety and process systems at TVO 1 and 2 nuclear power units was at first analyzed in a systematic way. The scope of the components studied was 81 MOVs in safety-related systems and 127 other MOVs per each TVO unit. In the case of the Loviisa plant, the observation period was limited to three years, i.e. from February 1989 up to February 1992. The scope of the Loviisa 1 and 2 components studied was 44 respectively 95 MOVs. (25 refs., 22 figs., 8 tabs.)

  7. 23 CFR 633.208 - Maintenance.

    Science.gov (United States)

    2010-04-01

    ... responsibility of the State. The State may arrange for maintenance of such roads or portions thereof, by... 23 Highways 1 2010-04-01 2010-04-01 false Maintenance. 633.208 Section 633.208 Highways FEDERAL... PROVISIONS Federal-Aid Contracts (Appalachian Contracts) § 633.208 Maintenance. Maintenance of all highway...

  8. Fast Flux Test Facility (FFTF) maintenance provisions

    International Nuclear Information System (INIS)

    Marshall, J.L.

    1981-05-01

    The Fast Flux Test Facility (FFTF) was designed with maintainability as a primary parameter, and facilities and provisions were designed into the plant to accommodate the maintenance function. This paper describes the FFTF and its systems. Special maintenance equipment and facilities for performing maintenance on radioactive components are discussed. Maintenance provisions designed into the plant to enhance maintainability are also described

  9. Lifetime oriented maintenance planning in the Netherlands

    NARCIS (Netherlands)

    Straub, A.

    2003-01-01

    In this paper we set up a framework for lifetime oriented maintenance planning as an outcome and input for strategic housing stock management. The maintenance planning holds maintenance activities and costs in the longer term. We consider the maintenance planning as a tool to calculate and implement

  10. A STEPPING STONE TOWARDS KNOWLEDGE BASED MAINTENANCE

    Directory of Open Access Journals (Sweden)

    G. Waeyenbergh

    2012-01-01

    Full Text Available Maintenance decision making becomes more and more a management concern. Some decades ago, maintenance was still often considered as an unavoidable side effect of production. The perception of maintenance has evolved considerably. One of the current issues is the maintenance concept, being the mix of maintenance interventions and the general framework for determining this mix. In this paper we describe a modular framework, called Knowledge Based Maintenance, for developing a customised maintenance concept. After describing the general framework and its decision support use, some case experiences are given. This experience covers some elements of the proposed framework.

  11. Diverless maintenance in deep water

    Energy Technology Data Exchange (ETDEWEB)

    Hals, B.E. [Oceaneering International Services, (United Kingdom)

    1996-12-31

    The topic of diverless maintenance will address subsea production facilities in this paper. The major factors that determine diverless maintainability are development economics, maintenance philosophy, subsea hardware design, subsea intervention hardware, and compatibility of subsea hardware and remote work capabilities. The opportunity for developing and implementing low cost diverless installable and maintenable subsea facilities exist. Designs, hardwares, and procedures exist althoughmany of these have been combined into powerful solutions. This paper addresses the issues that need to be considered and provides one set of solutions. There are a number of other solutions that can be combined in a different set to provide a parallel but different solution. The challenge is to establish a process that defines the requirement, identifies the resources/capabilities, executes the work. 3 refs., 10 figs.

  12. Total Productive Maintenance at Paccar INC

    Directory of Open Access Journals (Sweden)

    Ştefan Farkas

    2010-06-01

    Full Text Available This paper reports the application of total productive maintenance method at Paccar Inc. truck’s plant in Victoria, Australia. The total productive maintenance method and total productive maintenance house are presented. The global equipment effectiveness is computed and exemplified. The production structure and organising maintenance are presented. Resultas of the variation of global equipment effectiveness and autonomous maintenance in a two weeks period of time are reported.

  13. Total Productive Maintenance at Paccar INC

    OpenAIRE

    Ştefan Farkas

    2010-01-01

    This paper reports the application of total productive maintenance method at Paccar Inc. truck’s plant in Victoria, Australia. The total productive maintenance method and total productive maintenance house are presented. The global equipment effectiveness is computed and exemplified. The production structure and organising maintenance are presented. Resultas of the variation of global equipment effectiveness and autonomous maintenance in a two weeks period of time are reported.

  14. A probabilistic maintenance model for diesel engines

    Science.gov (United States)

    Pathirana, Shan; Abeygunawardane, Saranga Kumudu

    2018-02-01

    In this paper, a probabilistic maintenance model is developed for inspection based preventive maintenance of diesel engines based on the practical model concepts discussed in the literature. Developed model is solved using real data obtained from inspection and maintenance histories of diesel engines and experts' views. Reliability indices and costs were calculated for the present maintenance policy of diesel engines. A sensitivity analysis is conducted to observe the effect of inspection based preventive maintenance on the life cycle cost of diesel engines.

  15. GRAPHICAL MODELS OF THE AIRCRAFT MAINTENANCE PROCESS

    OpenAIRE

    Stanislav Vladimirovich Daletskiy; Stanislav Stanislavovich Daletskiy

    2017-01-01

    The aircraft maintenance is realized by a rapid sequence of maintenance organizational and technical states, its re- search and analysis are carried out by statistical methods. The maintenance process concludes aircraft technical states con- nected with the objective patterns of technical qualities changes of the aircraft as a maintenance object and organizational states which determine the subjective organization and planning process of aircraft using. The objective maintenance pro- cess is ...

  16. Building Maintenance Management System for Heritage Museum

    OpenAIRE

    Md Azree Othuman Mydin; Siti Hajar Ismail; Norhidayah Md Ulang

    2012-01-01

    An investment in the building maintenance aspect is massive throughout the world. In most of the countries, it signifies approximately 50% of the entire revenue of the construction industry. The value of buildings depends on the eminence of the maintenance invested in them. Maintenance management engages obtaining utmost advantage from the investment made on the maintenance activities. At the moment, maintenance in buildings in Malaysia is on the increase in spite of size, ...

  17. BWR Services maintenance training program

    International Nuclear Information System (INIS)

    Cox, J.H.; Chittenden, W.F.

    1979-01-01

    BWR Services has implemented a five-phase program to increase plant availability and capacity factor in operating BWR's. One phase of this program is establishing a maintenance training program on NSSS equipment; the scope encompasses maintenance on both mechanical equipment and electrical control and instrumentation equipment. The program utilizes actual product line equipment for practical Hands-on training. A total of 23 formal courses will be in place by the end of 1979. The General Electric Company is making a multimillion dollar investment in facilities to support this training. These facilities are described

  18. Forecasts of the Maintenance Activity

    Directory of Open Access Journals (Sweden)

    Marius Romocea

    2017-11-01

    Full Text Available Enhancing the machines’ technical and technological performance through constructive improvements that allow for the automation of orders, contributes to the increasing of the production capacity of the system and for maintaining an optimal level of product costs. In order to achieve these goals, it is necessary to organize the maintenance work well, in order to quickly diagnose the malfunctions and to shorten the repair time. Given the scale of the phenomena, this paper seeks to answer two questions, namely: how will be the maintenance in future and also how to prepare this activity.

  19. Plant inspection and maintenance technology

    International Nuclear Information System (INIS)

    Miyahara, Masatoshi; Kanazawa, Masafumi

    1995-01-01

    The article reports on information systems and related tools that support routine inspection and preventive maintenance activities, which contribute to safer plant operation and increase plant operating duty. Tools include the Mitsubishi Electric Reliability and Availability Upgrade Program-Nuclear (MELRAP-N), which supports the general evolution of industry operating experiences, periodic component replacement management, management of discontinued and modified components, periodic inspection item management, and periodic inspection workplace management. The article also introduces a comprehensive preventive maintenance management system currently under development. (author)

  20. Maintenance as a corporate strategy.

    Science.gov (United States)

    Clarke, D

    2000-11-01

    As we move towards the 21st century, those Manufacturing Industries that survive, will have changed their "efficiency" focus away from the conventional wisdom of "efficient individual tasks", to focus on the "effectiveness of a total manufacturing process". This seemingly insignificant change of emphasis, (from task to process) must include "the integration of process plant maintenance into the production process". Manufacturing companies, which do not include an effective plant maintenance strategy into their business strategy, will not survive the business environment of the 21st Century.

  1. Autophagy: a new player in skeletal maintenance?

    Science.gov (United States)

    Hocking, Lynne J; Whitehouse, Caroline; Helfrich, Miep H

    2012-07-01

    Imbalances between bone resorption and formation lie at the root of disorders such as osteoporosis, Paget's disease of bone (PDB), and osteopetrosis. Recently, genetic and functional studies have implicated proteins involved in autophagic protein degradation as important mediators of bone cell function in normal physiology and in pathology. Autophagy is the conserved process whereby aggregated proteins, intracellular pathogens, and damaged organelles are degraded and recycled. This process is important both for normal cellular quality control and in response to environmental or internal stressors, particularly in terminally-differentiated cells. Autophagic structures can also act as hubs for the spatial organization of recycling and synthetic process in secretory cells. Alterations to autophagy (reduction, hyperactivation, or impairment) are associated with a number of disorders, including neurodegenerative diseases and cancers, and are now being implicated in maintenance of skeletal homoeostasis. Here, we introduce the topic of autophagy, describe the new findings that are starting to emerge from the bone field, and consider the therapeutic potential of modifying this pathway for the treatment of age-related bone disorders. Copyright © 2012 American Society for Bone and Mineral Research.

  2. The maintenance management framework models and methods for complex systems maintenance

    CERN Document Server

    Crespo Márquez, Adolfo

    2010-01-01

    “The Maintenance Management Framework” describes and reviews the concept, process and framework of modern maintenance management of complex systems; concentrating specifically on modern modelling tools (deterministic and empirical) for maintenance planning and scheduling. It will be bought by engineers and professionals involved in maintenance management, maintenance engineering, operations management, quality, etc. as well as graduate students and researchers in this field.

  3. Numerical study of inventory management under various maintenance policies

    NARCIS (Netherlands)

    Poppe, J.; Basten, R.J.I.; Boute, R.N.; Lambrecht, M.R.

    2017-01-01

    Capital assets, such as manufacturing equipment, require maintenance to remain functioning. Maintenance can be performed when a component breaks down and needs replacement (i.e., corrective maintenance), or the maintenance and part replacement can be performed preventively. Preventive maintenance

  4. Methadone Maintenance as Last Resort

    DEFF Research Database (Denmark)

    Järvinen, Margaretha; Miller, Gale

    2010-01-01

    Drawing on qualitative interviews with drug addicts in Copenhagen, Denmark, this article offers a phenomenological reading of a methadone maintenance program. The program is set within the principles of harm reduction, meaning that its aim is not to cure the participants’ addiction but to keep them...

  5. Improving industrial maintenance contract relationships

    NARCIS (Netherlands)

    De Jong, A.

    2010-01-01

    Unavailability of technical systems, such as aircraft, is expensive. Technical systems require maintenance to prevent and to remedy defects that cause unavailability. Users of technical systems usually bear the consequences of the risk of unavailability, in particular the loss of productivity. This

  6. Project Surveillance and Maintenance Plan

    International Nuclear Information System (INIS)

    1985-09-01

    The Project Surveillance and Maintenance Plan (PSMP) describes the procedures that will be used by the US Department of Energy (DOE), or other agency as designated by the President to verify that inactive uranium tailings disposal facilities remain in compliance with licensing requirements and US Environmental Protection Agency (EPA) standards for remedial actions. The PSMP will be used as a guide for the development of individual Site Surveillance and Maintenance Plans (part of a license application) for each of the UMTRA Project sites. The PSMP is not intended to provide minimum requirements but rather to provide guidance in the selection of surveillance measures. For example, the plan acknowledges that ground-water monitoring may or may not be required and provides the [guidance] to make this decision. The Site Surveillance and Maintenance Plans (SSMPs) will form the basis for the licensing of the long-term surveillance and maintenance of each UMTRA Project site by the NRC. Therefore, the PSMP is a key milestone in the licensing process of all UMTRA Project sites. The Project Licensing Plan (DOE, 1984a) describes the licensing process. 11 refs., 22 figs., 8 tabs

  7. Maintenance of CERN telephone exchanges

    CERN Multimedia

    2005-01-01

    A maintenance of CERN telephone exchanges will be performed on 21st, 22nd, 23rd, 24th of September from 7 p.m. to 9 p.m. Disturbances or even interruptions of telephony services may occur during this lapse of time. We apology in advance for any inconveniences that this may cause.

  8. Maintenance as a safety issue.

    Science.gov (United States)

    White, Jim

    2008-11-01

    Because safety is related to electrical power systems maintenance, it seems reasonable to assume there could be legal issues if maintenance is not performed. OSHA has not yet taken the stand that not performing maintenance as required by the manufacturer, NFPA 70B, or ANSI/NETA MTS-07 constitutes a willful violation. OSHA defines a willful citation as one where: "the employer knowingly commits with plain indifference to the law. The employer either knows that what he or she is doing constitutes a violation, or is aware that a hazardous condition existed and made no reasonable effort to eliminate it". However, NFPA 70E 2009 requires this maintenance, and OSHA has stated on its Web site that NFPA 70E is "a guide for meeting the requirements of the OSHA electrical regulations". In addition, federal courts have found that NFPA 70E is "standard industry practice." Once a company receives and accepts a willful citation, especially if received as the result of an accident investigation, its worker's compensation protection no longer shields it. One definition given by a trial attorney for a willful citation was that it is equal to negligent behavior. Be smart: Maintain that equipment and save yourself major problems, including unscheduled shutdowns and possible litigation.

  9. Development of maintenance engineering system

    International Nuclear Information System (INIS)

    1995-03-01

    Department of JMTR project has developed the Maintenance Engineering System which evaluates the aging tendency of the facilities. The system is used for the repair plan and the replace period of parts, components, equipments or facilities. The system has the data-base which consists of the check data, the inspection data, the trouble data and the repair data at the JMTR since the virgin criticality. The system is utilized maintenance works and concludes the maintenance procedures for the failure components, equipments and facilities. This system has the following characteristics. (1) Anybody can operate the system as easily as word processor. (2) Data are put into by man-machine-interface. (3) The data sheets are with light color and the recognizable arrangements. (4) The system is cost-efficient using commercial personal computers and applications. The research card and the layouts of the input data sheet had been formatted. Data has been begun to be put into the system and to check its functions. The result demonstrates that the system is available for preventive maintenance at the JMTR. (author)

  10. Software Tools for Software Maintenance

    Science.gov (United States)

    1988-10-01

    COMMUNICATIONS, AND COMPUTER SCIENCES I ,(AIRMICS) FO~SOFTWARE TOOLS (.o FOR SOF1 ’ARE MAINTENANCE (ASQBG-1-89-001) October, 1988 DTIC ELECTE -ifB...SUNWW~. B..c Program An~Iysw HA.c C-Tractr C Cobol Stncturing Facility VS Cobol 11 F-Scan Foctma Futbol Cobol Fortran Sltiuc Code Anaiyaer Fortran IS

  11. Maintenance of remote reconnaissance vehicle

    International Nuclear Information System (INIS)

    Schein, D.C.

    1985-01-01

    A description is provided of the maintenance program developed for remote reconnaissance vehicles, such as RRV-1, in use at the Three Mile Island Unit 2. The described approach, which is simple, effective, and flexible, helped to make the effort successful. It will be applied to future projects

  12. Reflection of centrifugal pumps maintenance

    International Nuclear Information System (INIS)

    Mozos Fernandez, V.

    2010-01-01

    The aim of this work is to prove that is not necessary a deep and complex knowledge to manage the centrifugal pumps maintenance. According to the author, only deep but single technical knowledge about causes of breakdowns in the different and simple component parts of the pumps, are required. (Author)

  13. Building Maintenance. Student Learning Guide.

    Science.gov (United States)

    Palm Beach County Board of Public Instruction, West Palm Beach, FL.

    This student learning guide contains one module for completing a course in building maintenance. It is designed especially for use in secondary schools in Palm Beach County, Florida. The module covers one task, and consists of a purpose, performance objective, enabling objectives, learning activities keyed to resources, information sheets, student…

  14. Weight loss maintenance: A review on dietary related strategies

    Directory of Open Access Journals (Sweden)

    Fatemeh Azizi Soeliman

    2014-01-01

    Full Text Available Background: Weight regain after weight loss is a common problem for all those obese or overweight who have had a recent weight loss. Different cures such as diet therapy, behavioral therapy, exercise or a mixture of them have been advised as solutions. The purpose of this review is to find the best diet or eating pattern to maintain a recent weight loss. Materials and Methods: We searched in PubMed and SCOPUS by using the following key words: Overweight, obesity, weight maintenance, weight regain, and diet therapy. Finally, we assessed 26 articles in the present article. Results: Meal replacement, low carbohydrate-low glycemic index (GI diet, high protein intake, and moderate fat consumption have shown some positive effects on weight maintenance. However, the results are controversial. A Dietary Approach to Stop Hypertension (DASH-type diet seems helpful for weight maintenance although the need for more study has remained. Some special behaviors were associated with less weight regain, such as, not being awake late at night, drinking lower amount of sugar-sweetened beverages, and following a healthy pattern. Some special foods have been suggested for weight maintenance. However, the roles of specific foods are not confirmed. Conclusion: Healthy diets recommend low carbohydrate, low GI, and moderate fat foods, but it is not clear whether they are useful in preventing weight gain. It seems that consuming fewer calories helps people to keep weight loss. Further research to find strategies in obesity management focusing on successful maintenance of weight loss is needed.

  15. Remote maintenance systems requirements are being developed to provide design guidelines for machine components, to define maintenance interfaces, and to quantify maintenance equipment and procedures needed

    International Nuclear Information System (INIS)

    Spampinato, P.T.; Tabor, M.A.

    1988-01-01

    Remote maintenance systems requirements are being developed to provide design guidelines for machine components, to define maintenance interfaces, and to quantify maintenance equipment and procedures needed

  16. Operations and maintenance - Safety challenges

    Energy Technology Data Exchange (ETDEWEB)

    Nielsen, Liv [Oljedirektoratet, Stavanger (Norway)

    1999-07-01

    With the unsteady oil prices and the possible consequences of the deregulation of the European energy markets one may expect further optimization of operating and maintenance costs. One may also expect extended use of various risk-based optimization techniques such as RCM (Reliability Centered Maintenance) and RBI (Risk Based Inspection). This presentation addresses the need for further research and development in this area. Maintenance work is necessary, but it can also create risk. The accident statistics show many examples of this. The Norwegian petroleum industry's ability to learn from previous incidents is questioned. Maintenance staff must be well trained and possess the necessary routines. Technical documentation must be updated. Uncertainties with respect to future oil and gas prices combined with the effect of the deregulation of the European energy markets will lead to even more focus on cost-effective operations and maintenance. The need for long-term research and development is stressed. Risk based techniques such as RCM and RBI are extensively used in the defence industry and the nuclear industry, but applying them to the petroleum industry requires improved risk models. Ageing effects such as corrosion, erosion, fatigue etc. can be expected, but the capability to predict, monitor and control them should be improved. At present, not even the most sophisticated risk analysis can model ageing effects. The importance of efficient use of information technology (IT) is stressed. Improving the product quality and safety often requires new technology and so research and development is important. Close cooperation with the industry is required.

  17. Maintenance-Ready Web Application Development

    Directory of Open Access Journals (Sweden)

    Ion IVAN

    2016-01-01

    Full Text Available The current paper tackles the subject of developing maintenance-ready web applications. Maintenance is presented as a core stage in a web application’s lifecycle. The concept of maintenance-ready is defined in the context of web application development. Web application maintenance tasks types are enunciated and suitable task types are identified for further analysis. The research hypothesis is formulated based on a direct link between tackling maintenance in the development stage and reducing overall maintenance costs. A live maintenance-ready web application is presented and maintenance related aspects are highlighted. The web application’s features, that render it maintenance-ready, are emphasize. The cost of designing and building the web-application to be maintenance-ready are disclosed. The savings in maintenance development effort facilitated by maintenance ready features are also disclosed. Maintenance data is collected from 40 projects implemented by a web development company. Homogeneity and diversity of collected data is evaluated. A data sample is presented and the size and comprehensive nature of the entire dataset is depicted. Research hypothesis are validated and conclusions are formulated on the topic of developing maintenance-ready web applications. The limits of the research process which represented the basis for the current paper are enunciated. Future research topics are submitted for debate.

  18. GRAPHICAL MODELS OF THE AIRCRAFT MAINTENANCE PROCESS

    Directory of Open Access Journals (Sweden)

    Stanislav Vladimirovich Daletskiy

    2017-01-01

    Full Text Available The aircraft maintenance is realized by a rapid sequence of maintenance organizational and technical states, its re- search and analysis are carried out by statistical methods. The maintenance process concludes aircraft technical states con- nected with the objective patterns of technical qualities changes of the aircraft as a maintenance object and organizational states which determine the subjective organization and planning process of aircraft using. The objective maintenance pro- cess is realized in Maintenance and Repair System which does not include maintenance organization and planning and is a set of related elements: aircraft, Maintenance and Repair measures, executors and documentation that sets rules of their interaction for maintaining of the aircraft reliability and readiness for flight. The aircraft organizational and technical states are considered, their characteristics and heuristic estimates of connection in knots and arcs of graphs and of aircraft organi- zational states during regular maintenance and at technical state failure are given. It is shown that in real conditions of air- craft maintenance, planned aircraft technical state control and maintenance control through it, is only defined by Mainte- nance and Repair conditions at a given Maintenance and Repair type and form structures, and correspondingly by setting principles of Maintenance and Repair work types to the execution, due to maintenance, by aircraft and all its units mainte- nance and reconstruction strategies. The realization of planned Maintenance and Repair process determines the one of the constant maintenance component. The proposed graphical models allow to reveal quantitative correlations between graph knots to improve maintenance processes by statistical research methods, what reduces manning, timetable and expenses for providing safe civil aviation aircraft maintenance.

  19. Using heuristic search for optimizing maintenance plans

    International Nuclear Information System (INIS)

    Mutanen, Teemu

    2012-01-01

    This work addresses the maintenance action selection process. Maintenance personnel need to evaluate maintenance actions and costs to keep the machines in working condition. Group of actions are evaluated together as maintenance plans. The maintenance plans as output provide information to the user about which actions to take if any and what future actions should be prepared for. The heuristic search method is implemented as part of general use toolbox for analysis of measurements from movable work machines. Impacts from machine's usage restrictions and maintenance activities are analysed. The results show that once put on a temporal perspective, the prioritized order of the actions is different and provide additional information to the user.

  20. Maintenance planning for nuclear power plants

    International Nuclear Information System (INIS)

    Mattu, R.K.; Cooper, S.E.; Lauderdale, J.R.

    2004-01-01

    Maintenance planning for nuclear power plants is similar to that in other industrial plants but it is heavily influenced by regulatory rules, with consequent costs of compliance. Steps by the nuclear industry and the Nuclear Regulatory Commission to address that problem include development of guidelines for maintenance of risk-critical equipment, using PRA-based techniques to select a set of equipment that requires maintenance and reliability-centered maintenance (RCM) approaches for determining what maintenance is required. The result of the process is a program designed to ensure effective maintenance of the equipment most critical to plant safety. (author)

  1. Status of online maintenance in the world

    International Nuclear Information System (INIS)

    Mizumachi, Wataru; Kobayashi, Masahide

    2009-01-01

    In many countries, online maintenance has been already introduced. Especially in US, online maintenance has been introduced to many nuclear plants which has (N+1) redundant safety systems as Japanese plants. There experience will contribute us to discuss how to introduce online maintenance in Japan. In IAEA's nuclear safety criteria and guidance, it is recognized that the outage of one safety system for maintenance is usual operation. This is normal sense in the world. In US, the risk when online maintenance is done is controlled mainly by PRA. We have to discuss how to introduce online maintenance in JAPAN under these conditions in the world. (author)

  2. Reliability-based maintenance evaluations and standard preventive maintenance programs

    International Nuclear Information System (INIS)

    Varno, M.; McKinley, M.

    1993-01-01

    Due to recent issuance of 10CFR50.65, the U.S. Nuclear Regulatory Commission maintenance rule (Rule), and the open-quotes Industry Guideline for Monitoring the Effectiveness of Maintenance at Nuclear Power Plantsclose quotes prepared by the Nuclear Management and Resources Council, many utilities are undertaking review or evaluation of current preventive maintenance (PM) programs. Although PM optimization and documentation are not specifically required by the Rule, an appropriate and effective PM program (PMP) will be the cornerstone of the successful and cost-effective implementation of the Rule. Currently, a project is being conducted at the Vermont Yankee Nuclear Power Station (VYNPS) in conjunction with Quadrex Energy Services to evaluate, optimize, and document the PMP. The project began in March 1993 and is scheduled for completion in mid-1995. The initial scope for the project is the evaluation of those structures, systems, and components that are within the scope of the Rule. Because of the number of systems to be evaluated (∼50), the desired completion schedule, and cost considerations, a streamlined approach to PM optimization and documentation is being utilized

  3. CHILD MAINTENANCE IN TURKISH LAW

    Directory of Open Access Journals (Sweden)

    Banu Bilge Sarihan

    2017-12-01

    Full Text Available The concept of alimony; the dictionary defines as, the whole of what is needed to make a living; as the legal sense is defined as connected with one month to one court decision that obliged to provide for. Family members of a moral rule, first of all to help each other. This moral without often any coercion parties in the framework adapts to this rule, but in this case the legislator for the processing always smoothly, has made it a statutory duty by going to road regulations to help each other for certain family members. Assistance in the form of alimony and child support maintenance can be divided into two main groups. Support resulting from family law, commonly referred to as maintenance support. Maintenance alimony; temporary alimony, child maintenance and poverty alimony. The care and upbringing of children in the marital union is entitled to custody of the mother and father in the framework. Mother and father use custody together. Custody of minors and adult children must sometimes restricted to persons, about paying attention to both the goods and to represent them as a whole of the rights and obligations of the law have been installed on the parents. Common life of the spouses or by court order issued at the end of separation has occurred judge may give custody to one of the spouses. side with custody of children have been left to him is obliged to look after and educate them. However, not given custody of his side, must participate in their child's care and education expenses amount to be determined by the judge according to financial strength. Associates alimony, separation or nullity or divorce, child custody as a result of which he had left his wife, child care and the financial strength to participate in the rate training expenses. Associates alimony, not a liability connected to custody, is a natural consequence of being parents. Because spouses are obliged to take care of children's care and upbringing. Child maintenance is

  4. Maintenance of nuclear power plants

    International Nuclear Information System (INIS)

    Lashgari, Farbod.

    1995-01-01

    This paper is about maintenance of nuclear power plants. In part one, the outage management of nuclear power plants has described. Meaning of the outage and objectives of outage management is given in introduction. The necessity of a long-term outage strategy is shown in chapter one. The main parts of an outage are as follows: Planning; Preparation; Execution, Each of them and also post-outage review have been explained in the followed chapters. Part two deals with technical details of main primary components of nuclear power plant type WWER. After an introduction about WWER reactors, in each chapter first the general and detailed description of main primary components has given and then their maintenance schedules and procedures. Chapter about reactor and steam generator is related to both types of WWER-440 and WWER-1000, but chapter about reactor coolant pump has specified to WWER-1000 to be more in details.(author)

  5. Alternative cask maintenance facility concepts

    International Nuclear Information System (INIS)

    Attaway, C.R.; Pope, R.B.; Wiliamson, A.C.; Medley, L.G.; Shappert, L.B.

    1992-01-01

    In this paper, the results of three trade-off studies of alternative concepts for performing cask maintenance for Civilian Radioactive Waste Management System casks are presented. An earlier study resulted in a recommendation that a submerged pool concept for cask internal component removal be used in the design of a Cask Maintenance Facility. The first trade-off study resulted in confirming the previous recommendation that a submerged pool concept be used rather than an isolation cell; the basis for this continued recommendation is discussed. The second study provides an evaluation of the previously proposed facility for the capability of handling an increased quantity of OCRWM casks. The third study provides a preliminary concept for adding the capability to repaint the exterior cylindrical portions of casks

  6. The Nordic maintenance care program

    DEFF Research Database (Denmark)

    Malmqvist, Stefan; Leboeuf-Yde, Charlotte

    2009-01-01

    of maintenance care. Previous studies have identified chiropractors' choices of case management strategies in response to different case scenarios. However, the rationale for these management strategies is not known. In other words, when presented with both the case, and different management strategies......Maintenance care is a well known concept among chiropractors, although there is little knowledge about its exact definition, its indications and usefulness. As an initial step in a research program on this phenomenon, it was necessary to identify chiropractors' rationale for their use......, there was consensus on how to match these, but if only the management strategies were provided, would chiropractors be able to define the cases to fit these strategies? The objective with this study was to investigate if there is a common pattern in Finnish chiropractors' case management of patients with low back...

  7. Maintenance facilities, stores and records

    International Nuclear Information System (INIS)

    Fischer, K.

    1986-01-01

    The topics of this report are: on-site or off-site facilities. On-site facilities: workshops and special facilities. KWU's Service Center, a typical off-site supporting facility in Germany, capabilities and activities. A pool for special tools and equipment: devices for plugging of nozzles, for handling of RPU-studs and RPU internals etc. Devices for and management of radiological protection on-site for typical outage work. Spare parts and spare part management on site, typical examples. KWU's centralized spare part pools for components, generators and turbines etc. A computerized system for spare parts storage and maintenance planning. A system for mutual exchange of operational experience with respect to maintenance and repair activities. Systematic evaluation of failures and statistical results. (orig./GL)

  8. Procedures for maintenance and repairs

    International Nuclear Information System (INIS)

    Pickel, E.

    1981-01-01

    After a general review of the operation experience in the history of more than 12 operating years, the organization in the plant will be shown with special aspect to quality assurance, capacity of the workshops and connected groups as radiation protection, chemical laboratories etc. The number, time intervals and manpower effort for the repeating tests will be discussed. Reasons and examples for back-fitting activities in the plant are given. Besides special repair and maintenance procedures as repair of the steam generators, in-service inspection of the reactor pressure vessel, repair of a feed-water pipe and repair of the core structure in the pressure vessel, the general system to handle maintenance and repair-work in the KWO-plant will be shown. This includes also the detailed planning of the annual refueling and revision of the plant. (orig./RW)

  9. BWR nuclear plant maintenance simulation

    International Nuclear Information System (INIS)

    Stuart, I.F.

    1985-01-01

    As early as 1977, the General Electric Company, USA, Nuclear Energy Operation was making plans to construct a maintenance-type simulator to support Training and Services. The Company's pioneering experience with control room simulators started in 1968 with the Dresden simulator and showed clearly the benefits of having such facilities for training, checkout of procedures and, in the case of maintenance, match-up of equipment or tools as needed. Since the dedication of the facility, it has proved to be an invaluable resource in the training of refuelling and servicing crews. The facility has also been extensively used as developmental and test facility for in-vessel servicing equipment and procedures. (author)

  10. Maintenance training - a modern necessity

    International Nuclear Information System (INIS)

    Bushall, W.

    1987-01-01

    In recent years, there has been an increase in technically advanced systems and equipment and a need for highly skilled and knowledgeable maintenance technicians to maintain them. To implement an effective training program, training groups' and plant staffs' key to success must be cooperation and creativity. This paper deals with plant staff interface and how to effectively conduct performance-based training while holding the line on costs. This paper includes cost effective and innovative measures to produce performance-based training for maintenance disciplines including: Using the plants staff as a resource as subject matter experts in the development and verification of training materials. Using the plant staff as a resource for the construction of training aids. Using salvage and surplus to produce high quality, low cost training aids. Using cutaways for better understanding of he theory of equipment operation. These cost saving practices are currently being used at Gulf States Utilities' River Bend Nuclear Station

  11. Mining Surveillance and Maintenance Dollars

    International Nuclear Information System (INIS)

    MARTINEZ, R.

    2000-01-01

    Accelerating site cleanup to reduce facility risks to the workers, the public and the environment during a time of declining federal budgets represents a significant technical and economic challenge to U.S. Department of Energy (DOE) Operations Offices and their respective contractors. A significant portion of a facility's recurring annual expenses are associated with routine, long-term surveillance and maintenance (S and M) activities. However, ongoing S and M activities do nothing to reduce risks and basically spend money that could be reallocated towards facility deactivation. This paper discusses the background around DOE efforts to reduce surveillance and maintenance costs, one approach used to perform cost reviews, lessons learned from field implementation and what assistance is available to assist DOE sites in performing these evaluations

  12. Design for maintenance during operation

    International Nuclear Information System (INIS)

    Barton, P.H.; Lyerly, H.D.

    1976-01-01

    Design of a nuclear power plant for safe, reliable and efficient operation involves much more than theory, regulations, regulatory guides, procedures, inspectors, and even quality assurance. It also involves a full knowledge and understanding of the activities which must be carried out to operate and maintain the plant. It must be remembered that a nuclear power plant is different from fossil designs and the designer must constantly keep in mind that access by people to every component is required for maintenance

  13. Scheduling Maintenance Jobs in Networks

    OpenAIRE

    Abed, Fidaa; Chen, Lin; Disser, Yann; Groß, Martin; Megow, Nicole; Meißner, Julie; Richter, Alexander T.; Rischke, Roman

    2017-01-01

    We investigate the problem of scheduling the maintenance of edges in a network, motivated by the goal of minimizing outages in transportation or telecommunication networks. We focus on maintaining connectivity between two nodes over time; for the special case of path networks, this is related to the problem of minimizing the busy time of machines. We show that the problem can be solved in polynomial time in arbitrary networks if preemption is allowed. If preemption is restricted to integral t...

  14. Telomere Maintenance Mechanisms in Cancer

    OpenAIRE

    Tiago Bordeira Gaspar; Ana Sá; José Manuel Lopes; Manuel Sobrinho-Simões; Paula Soares; João Vinagre

    2018-01-01

    Tumour cells can adopt telomere maintenance mechanisms (TMMs) to avoid telomere shortening, an inevitable process due to successive cell divisions. In most tumour cells, telomere length (TL) is maintained by reactivation of telomerase, while a small part acquires immortality through the telomerase-independent alternative lengthening of telomeres (ALT) mechanism. In the last years, a great amount of data was generated, and different TMMs were reported and explained in detail, benefiting from g...

  15. Improving Minuteman III Maintenance Concepts

    Science.gov (United States)

    2017-03-23

    the ground . Then figure out what your challenges are. We’ll have those” (Pappalardo, 2011). Another challenge facing the maintenance personnel...as assets aged as indicated by the pattern ‘B’ shown in Figure 2. With the increase in the mechanization of processes, more attention has been...concepts could be applied to the sustainment of the MM III as well as benefit its planned replacement, the Ground Based Strategic Deterrent (GBDS

  16. Chromatin replication and epigenome maintenance

    DEFF Research Database (Denmark)

    Alabert, Constance; Groth, Anja

    2012-01-01

    Stability and function of eukaryotic genomes are closely linked to chromatin structure and organization. During cell division the entire genome must be accurately replicated and the chromatin landscape reproduced on new DNA. Chromatin and nuclear structure influence where and when DNA replication...... initiates, whereas the replication process itself disrupts chromatin and challenges established patterns of genome regulation. Specialized replication-coupled mechanisms assemble new DNA into chromatin, but epigenome maintenance is a continuous process taking place throughout the cell cycle. If DNA...

  17. Dynamic contracting mechanism for pavement maintenance management

    NARCIS (Netherlands)

    Demirel, H.C.; De Ridder, H.A.J.

    2013-01-01

    Technological advances, financial possibilities and changes of demands have increasingly affected the pavement maintenance environment for outsourcing in recent years. This induces complexity in the contracting methods of pavement maintenance activities. Despite the fact that current contracting

  18. FAA Certificated Maintenance Agencies Directory (1997)

    Science.gov (United States)

    1997-05-06

    This advisory circular (AC) transmits a consolidated directory of all : certificated Federal Aviation Administration (FAA) repair stations and : manufacturer's maintenance facilities. The repair stations and manufacturer's : maintenance facilities we...

  19. Machine maintenance: the rules of the game

    CERN Multimedia

    Antonella Del Rosso

    2013-01-01

    GCO, GMIO, GSPO – ever heard of them? These acronyms stand for the “roles” that best practices identify as vital to successful maintenance management. CERN’s Maintenance Management Project (MMP) team is working with the technical departments to ensure that they implement these procedures when they deal with the maintenance of the collider and its injectors and infrastructure.   The participants of the workshop on asset and maintenance management (AMMW2013), which took place at CERN in November. Since its creation in 2012, CERN’s Maintenance Framework Implementation Office (MFIO) has been promoting the deployment of harmonised maintenance management methods across the BE, GS, EN and TE Departments. “The definition and identification of the Group Coding Officers (GCO), Group Maintenance Information Officers (GMIO) and Group Spare Part Officers (GSPO) are one of the best practices that should be adopted each time a maintenance programme...

  20. Maintenance program, organization and procedures

    International Nuclear Information System (INIS)

    Schegk, J.R.R.

    1986-01-01

    The Rheinisch-Westfaelisches Elektrizitaetswerk AG (RWE) operates two pressurized water reactors (PWR) with 1.200 MW and 1.300 MW and two boiling water reactors (BWR) with an electrical output of 1.300 MW each. The PWR were commissioned in 1975 and 1977 respectively and have so far achieved a cumulative availability of 72%. The BWR started operating in 1984 and 1985. Since than they have been generating with a cumulative availability of 88%. The reliability of the nuclear power stations resulted in a trouble-free operation. For the last two years, the unscheduled non-availability amounted to 3,7% and 6,5% for the PWR and the BWR respectively. These favourable operating results derive inter alia from systematic preventive maintenance and consistent outage management. Apart from the personnel structure in the power stations the functional sequence of planning, control, performance as well as evaluation of maintenance results constitute a closed cycle. In order to ensure and support an effective cooperation of the above mentioned four functional sequences of a cycle, uniform and computer-assisted information and planning aids have been introduced such as task lists, job ordering, time scheduling and materials management. As a result of this preventive maintenance system the average refueling outage period is 35 to 42 days. (orig.)

  1. On condition based maintenance policy

    Directory of Open Access Journals (Sweden)

    Jong-Ho Shin

    2015-04-01

    Full Text Available In the case of a high-valuable asset, the Operation and Maintenance (O&M phase requires heavy charges and more efforts than the installation (construction phase, because it has long usage life and any accident of an asset during this period causes catastrophic damage to an industry. Recently, with the advent of emerging Information Communication Technologies (ICTs, we can get the visibility of asset status information during its usage period. It gives us new challenging issues for improving the efficiency of asset operations. One issue is to implement the Condition-Based Maintenance (CBM approach that makes a diagnosis of the asset status based on wire or wireless monitored data, predicts the assets abnormality, and executes suitable maintenance actions such as repair and replacement before serious problems happen. In this study, we have addressed several aspects of CBM approach: definition, related international standards, procedure, and techniques with the introduction of some relevant case studies that we have carried out.

  2. Activities of maintenance and outage

    International Nuclear Information System (INIS)

    Gracia-Orellan, J. M.; Gonzalez, P. L.; Verdu, M. F.; Fernandez, J. A.

    2004-01-01

    Iberinco Nuclear Generation Department have wanted to promote service activities in nuclear power plants for years besides its dedication to engineering activities. for it, in 1997 Nuclear Services Management was created to complement engineering activities and to be able to make an offer for products and turn key services. People involved in this Management have an extensive experience in Services Area, so that all type of maintenance works are promoted, as well other services like dismantling, fallout management and new equipment's for nuclear power plants services. Iberinco's experience in Support Services in Nuclear Power Plants allows to answer effectively to special workers during operation cycle and outages periods. These activities have been made in spanish nuclear power plants and Angra I and II plants, both of them in Brazil. In this area we provide Technical Consulting Management and Supervision to develop the following activities: - Improvement Maintenance Programs based in PSA: Implantation of Maintenance Rule in the plants. - Supervision and Assembly of design modifications in structures, systems and components. - Fulfilment of efficiency tests, inspection and turbo-group modification. - Noise and vibrations analysis. - Valves and rotative equipment calculations and diagnosis tests. Iberinco develop these outage activities itself or as contractors coordinator under its management. A lot of them have been working with Iberinco for many years and have a great experience in the Service Area they are developing. In this article, the main outage activities developed for Iberinco are detailed. (Author)

  3. Maintenance of nuclear medicine instruments

    Energy Technology Data Exchange (ETDEWEB)

    Ambro, P

    1993-12-31

    Maintenance of instruments is generally of two kinds: (a) corrective maintenance, on a non-scheduled basis, to restore equipment to a functional status by repairs; (b) preventive maintenance, to keep equipment in a specified functional condition by providing systematic inspection, quality control, detection and correction of early malfunctions. Most of the instruments used in nuclear medicine are rather complex systems built from mechanical, electrical and electronic parts. Any one of these components is liable to fail at some time or other. Repair could be done only by a specialist who is able to evaluate the condition of the various parts ranging from cables to connectors, from scintillators to photomultipliers, from microprocessors to microswitches. The knowledge of the intricacies of the various electronic components required for their repairs is quite wide and varied. The electronics industry turns out more and more multi-purpose chips which can carry out the functions of many parts used in the instruments of the earlier generation. This provides protection against unauthorized copying of the circuits but it serves another purpose as well of inhibiting repairs by non-factory personnel. These trends of the instrument design should be taken into consideration when a policy has to be developed for the repairs of the hospital based equipment 1 fig., 1 tab

  4. Maintenance of nuclear medicine instruments

    International Nuclear Information System (INIS)

    Ambro, P.

    1992-01-01

    Maintenance of instruments is generally of two kinds: (a) corrective maintenance, on a non-scheduled basis, to restore equipment to a functional status by repairs; (b) preventive maintenance, to keep equipment in a specified functional condition by providing systematic inspection, quality control, detection and correction of early malfunctions. Most of the instruments used in nuclear medicine are rather complex systems built from mechanical, electrical and electronic parts. Any one of these components is liable to fail at some time or other. Repair could be done only by a specialist who is able to evaluate the condition of the various parts ranging from cables to connectors, from scintillators to photomultipliers, from microprocessors to microswitches. The knowledge of the intricacies of the various electronic components required for their repairs is quite wide and varied. The electronics industry turns out more and more multi-purpose chips which can carry out the functions of many parts used in the instruments of the earlier generation. This provides protection against unauthorized copying of the circuits but it serves another purpose as well of inhibiting repairs by non-factory personnel. These trends of the instrument design should be taken into consideration when a policy has to be developed for the repairs of the hospital based equipment

  5. Demonstration of reliability centered maintenance

    International Nuclear Information System (INIS)

    Schwan, C.A.; Morgan, T.A.

    1991-04-01

    Reliability centered maintenance (RCM) is an approach to preventive maintenance planning and evaluation that has been used successfully by other industries, most notably the airlines and military. Now EPRI is demonstrating RCM in the commercial nuclear power industry. Just completed are large-scale, two-year demonstrations at Rochester Gas ampersand Electric (Ginna Nuclear Power Station) and Southern California Edison (San Onofre Nuclear Generating Station). Both demonstrations were begun in the spring of 1988. At each plant, RCM was performed on 12 to 21 major systems. Both demonstrations determined that RCM is an appropriate means to optimize a PM program and improve nuclear plant preventive maintenance on a large scale. Such favorable results had been suggested by three earlier EPRI pilot studies at Florida Power ampersand Light, Duke Power, and Southern California Edison. EPRI selected the Ginna and San Onofre sites because, together, they represent a broad range of utility and plant size, plant organization, plant age, and histories of availability and reliability. Significant steps in each demonstration included: selecting and prioritizing plant systems for RCM evaluation; performing the RCM evaluation steps on selected systems; evaluating the RCM recommendations by a multi-disciplinary task force; implementing the RCM recommendations; establishing a system to track and verify the RCM benefits; and establishing procedures to update the RCM bases and recommendations with time (a living program). 7 refs., 1 tab

  6. Development of preventive maintenance procedures and schedules

    International Nuclear Information System (INIS)

    Schlenker, H.V.; Hammel, J.

    1977-01-01

    An outline of the procedures and schedules developed for preventive maintenance in power stations within the Rheinisch-Westfaelisches Elektrizitaetswerk (RWE) is presented. After an introduction of maintenance in general the different kinds of preventive maintenance activities are described. This includes also the prerequisite identification systems. The aims of preventive maintenance and the measures are explained that have to be taken, if these aims are to be achieved. A number of examples from actual practice are cited. (orig.) [de

  7. Knowledge maintenance in myCBR

    OpenAIRE

    Aul, Vani; Sauer, Christian; Roth-Berghofer, Thomas

    2014-01-01

    CBR systems, being knowledge based systems, process knowledge. Due to changes in the environment a CBR system’s knowledge model can become outdated, thus creating a need for constant maintenance of said knowledge model. In this paper, we describe an implementation of (semi-)automatic knowledge maintenance of two of the four knowledge containers of CBR systems, specifically case base maintenance and maintenance of similarity measures within the CBR system development SDK myCBR. We describe our...

  8. Proving maintenance practices at France's CETIC facility

    International Nuclear Information System (INIS)

    Anon.

    1987-01-01

    CETIC, a PWR maintenance testing, training and validation centre became operational in September 1986. It is designed to meet the following basic requirements: development of plant maintenance processes to reduce work time, validation of tools for use during maintenance, training and qualification of teams for performing high-technology, high-risk operations in nuclear power plants. (U.K.)

  9. 14 CFR 145.217 - Contract maintenance.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Contract maintenance. 145.217 Section 145...) SCHOOLS AND OTHER CERTIFICATED AGENCIES REPAIR STATIONS Operating Rules § 145.217 Contract maintenance. (a) A certificated repair station may contract a maintenance function pertaining to an article to an...

  10. Building Maintenance Management System for Heritage Museum

    Directory of Open Access Journals (Sweden)

    Md Azree Othuman Mydin

    2012-09-01

    Full Text Available An investment in the building maintenance aspect is massive throughout the world. In most of the countries, it signifies approximately 50% of the entire revenue of the construction industry. The value of buildings depends on the eminence of the maintenance invested in them. Maintenance management engages obtaining utmost advantage from the investment made on the maintenance activities. At the moment, maintenance in buildings in Malaysia is on the increase in spite of size, category, location, and ownership. This study focuses on Building Maintenance Management System for Heritage Museum, which consists of two case studies in Penang State Museum and Art Gallery, Malaysia and Museum of Perak, Malaysia. The aim of this study is to propose methods to improve the maintenance management system for heritage museum. From the results, the common problem occurs during the implementation for the maintenance of each building is the budget for the maintenance and worker’s skill. The department of each museum must have their own maintenance unit to keep an eye on the maintenance activities for their buildings in order to improve the maintenance management system in their building.

  11. Structuring AHP-based maintenance policy selection

    NARCIS (Netherlands)

    Goossens, Adriaan; Basten, Robertus Johannes Ida; Hummel, J. Marjan; van der Wegen, Leonardus L.M.

    2015-01-01

    We aim to structure the maintenance policy selection process for ships, using the Analytic Hierarchy Process (AHP). Maintenance is an important contributor to reach the intended life-time of capital technical assets, and it is gaining increasing interest and relevance. A maintenance policy is a

  12. Selecting Suitable Candidates for Predictive Maintenance

    NARCIS (Netherlands)

    Tiddens, Wieger Willem; Braaksma, Anne Johannes Jan; Tinga, Tiedo

    2018-01-01

    Predictive maintenance (PdM) or Prognostics and Health Management (PHM) assists in better predicting the future state of physical assets and making timely and better-informed maintenance decisions. Many companies nowadays ambition the implementation of such an advanced maintenance policy. However,

  13. A COMPUTER BASED MAINTENANCE MANAGEMENT SYSTEM ...

    African Journals Online (AJOL)

    ... programs to schedule for maintenance or replacement of machines has been designed such that it enables the maintenance department control all jobs associated with plant maintenance and breakdown. It predicts the time to failure of designated plants and caters for the replacement analysis of some capital equipment.

  14. Maintenance considerations of the STARFIRE commercial tokamak

    International Nuclear Information System (INIS)

    Trachsel, C.A.; Zahn, H.S.; Field, R.E.; Stevens, H.C.

    1979-01-01

    This paper presents the maintenance approach, the commercial tokamak design features that enhance maintenance and preliminary repair time and required mean-time-between-failures for major subsystems. Reactor hall building and maintenance equipment requirements including hot cells, coil rewinding, and cranes are discussed

  15. Computerizing Maintenance Management Improves School Processes.

    Science.gov (United States)

    Conroy, Pat

    2002-01-01

    Describes how a Computerized Maintenance Management System (CMMS), a centralized maintenance operations database that facilitates work order procedures and staff directives, can help individual school campuses and school districts to manage maintenance. Presents the benefits of CMMS and things to consider in CMMS selection. (EV)

  16. An effective manpower planning approach for maintenance ...

    African Journals Online (AJOL)

    The level of maintenance activities of a cocoa processing factory in Nigeria was studied. Data on critical machine parts involved in the maintenance work were collected and the different jobs carried out according to sections and nature of the activities were classified. The workloads for each type of maintenance activities ...

  17. Collaborative learning in condition based maintenance

    NARCIS (Netherlands)

    Koochaki, J.; Ao, SI; Gelman, L; Hukins, DWL; Hunter, A; Korsunsky, AM

    2009-01-01

    In recent years, the importance of reliable and consistent production equipments has increased. As a result, companies are shifting their maintenance policy from preventive maintenance towards Condition Based Maintenance (CBM). Despite the growing trend in this area and success stories of CBM

  18. Maintenance rule: a system to guarantee maintenance effectiveness

    International Nuclear Information System (INIS)

    Torralbo, J.R.

    1995-01-01

    In the last few years, maintenance activities in nuclear plants have been given prime attention in view of their repercussion on the performance of such plants. One of the results of that attention is a new rule published in the U.S. known as M aintenance Rule . The paper describes the philosophy and main guidelines of the new regulation, and presents the procedure brought into action by the nuclear industry in Spain so to make their application contribute to a real improvement of safety and performance in nuclear plants. (Author)

  19. With a re-engineering of maintenance to a profitably oriented maintenance

    International Nuclear Information System (INIS)

    Angelevska, Silvana; Jolevski, Tome

    2000-01-01

    This paper presents an overview of effectiveness of the profit centered maintenance as a contemporary way of the maintenance management concept. Such redesign of a maintenance procedure results in significant gains in cost reduction and increase of the profitability and competitiveness of energy. The business process re engineering - a modern management control theory that is important aspect of the profit centered maintenance is summarized

  20. Maintenance philosophy and program at Cernavoda NPP

    International Nuclear Information System (INIS)

    Bobos, M.; Enciu, G.

    1994-01-01

    Maintenance plays a key role in ensuring safe and reliable operation. An effective maintenance program should ensure that installed equipment operates when needed and the equipment malfunctions or deficiencies are corrected in time and rarely recur. Maintenance includes not only the activities traditionally associated with identifying or correcting current or potential equipment deficiencies but also extends to supporting technical functions for the conduct of these activities (for example, engineering, technical support, chemistry control, radiological protection, industrial safety and training). The maintenance management program should clearly define the relationship among these supporting groups as it is related to overall plant maintenance and promotes the concept of a successful integrated team effort. (Author)

  1. Electric motor predictive and preventive maintenance guide

    International Nuclear Information System (INIS)

    Oliver, J.A.

    1992-07-01

    Electric motor performance is vital to the reliable and efficient operation of power plants. The failure of one or more critical motors could cause lost capacity and excessive repair and maintenance cost. However, existing maintenance recommendations proposed by vendors for electric motors have sometimes encouraged many overly conservative maintenance practices. These practices have lead to excessive maintenance activities and costs which have provided no extra margin of operability. EPRI has sponsored RP2814-35 to develop a guide which provides power plants with information and guidance for establishing an effective maintenance program which will aid in preventing unexpected motor failures and assist in planning motor maintenance efforts. The guide includes a technical description which summarizes technical data relative to the four basic types of motors and their components in general use in power plants. The significant causes of motor failures are investigated and described in detail and methods to optimize service life and minimize maintenance cost through appropriate preventive maintenance and conditioning program are presented. This guide provides a foundation for an effective electric motor maintenance program and simplifies the selection of predictive and preventive maintenance tasks. Its use will enable maintenance personnel in nuclear and fossil plants to plan motor repairs during scheduled outages and avoid costly unexpected failures

  2. Pacific Northwest Laboratory Maintenance Implementation plan

    International Nuclear Information System (INIS)

    Bright, J.D.

    1992-06-01

    This Maintenance Implementation plan has been developed for Pacific Northwest Laboratory's (PNL) Nuclear Facilities: 306W, 324, 325, 327 and 329NMF. It is based on a graded approach, self-assessment of the existing maintenance program(s) per the requirements specified by US Department of Energy (DOE) Order 4330.4A, Chapter II, Change number-sign 3. The results of this assessment were evaluated to determine needed improvements in PNL Craft Services' current maintenance program. The objective of this implementation plan is to provide baseline information for compliance to the DOE 4330.4A, and for needed improvements. The prime consideration in applying a graded approach to the Order has been to maintain safe and reliable operations, environmental compliance, safeguards and security, programmatic mission, facility preservation, and/or other facility-specific requirements. Using the results of the self-assessment, PNL has selected nine of the 18 elements of the Maintenance Program defined by DOE Order 4330.4A for improvement. The elements selected for improvement are Training and Qualification of Maintenance Personnel; Maintenance Procedures; Planning, Scheduling, and Coordination of Maintenance; Control of Maintenance Activities; Post-Maintenance Testing; Facility Condition Inspection; Management Involvement; Maintenance History; and Additional Maintenance Requirements. Based upon graded approach and current funding, those elements considered most important have been selected as goals for earliest compliance. Commitment dates for these elements have been established for compliance. The remaining elements of noncompliance will be targeted for implementation during later budget periods

  3. From structure to mechanism—understanding initiation of DNA replication

    Science.gov (United States)

    Riera, Alberto; Barbon, Marta; Noguchi, Yasunori; Reuter, L. Maximilian; Schneider, Sarah; Speck, Christian

    2017-01-01

    DNA replication results in the doubling of the genome prior to cell division. This process requires the assembly of 50 or more protein factors into a replication fork. Here, we review recent structural and biochemical insights that start to explain how specific proteins recognize DNA replication origins, load the replicative helicase on DNA, unwind DNA, synthesize new DNA strands, and reassemble chromatin. We focus on the minichromosome maintenance (MCM2–7) proteins, which form the core of the eukaryotic replication fork, as this complex undergoes major structural rearrangements in order to engage with DNA, regulate its DNA-unwinding activity, and maintain genome stability. PMID:28717046

  4. Susquehanna SES maintenance supervisor training and certification

    International Nuclear Information System (INIS)

    Deckman, M.

    1991-01-01

    Susquehanna's program targets all Supervisors, Supervisor Candidates, and Temporary Supervisors that are responsible for in-plant maintenance or maintenance support activities, including: mechanical maintenance; electrical maintenance; maintenance support (labor support, radwaste, etc.); mobile construction support (mechanical and electrical); chemistry; health physics; maintenance planning; and instrument and controls. The program integrates the three major areas of direct Supervisory responsibilities: (1) Leadership and Management - Skills that require interpersonal activities that are typically humanistic and subjective; such as coaching, motivating, communications, etc. (2) Technical and Administrative - Knowledge that is directly related to the job of Supervising from the production, regulatory, accountability perspective. These topics are very objective and include training on topics such as workpackages, plant chemistry parameters, radiological concerns, etc. (3) Technical Skills - Ensure each Supervisor is technically competent in the plant systems, components, or equipment he/she is tasked with maintaining or overseeing. Typical skills found in this area are, circuit breaker maintenance, primary system sampling, or overhauling pumps

  5. Lean thinking for a maintenance process

    Directory of Open Access Journals (Sweden)

    Sherif Mostafa

    2015-01-01

    Full Text Available The maintenance process shares significant operating costs in an organisation. Lean thinking can be incorporated into maintenance activities through applying its principles and practices/tools. Lean maintenance (LM is a prerequisite for lean manufacturing systems. This research proposes a new structure for LM process based on a systematic literature review of a significant number of related articles that were published on LM. The process structure is designed based on the five lean principles to guide and support organisations to pursue maintenance excellence. This study establishes a scheme for LM tools that are structured into 2 level 4 bundles and 26 lean practices/tools and develops a House of Waste (HoW to demonstrate the association between maintenance wastes and the LM tools. With a successful accomplishment of the proposed scheme, the performance of a maintenance department can create more improvement opportunities over time to reach the maintenance excellence status.

  6. Development of blanket remote maintenance system

    International Nuclear Information System (INIS)

    Kakudate, Satoshi; Nakahira, Masataka; Oka, Kiyoshi; Taguchi, Kou

    1998-01-01

    ITER in-vessel components such as blankets are scheduled maintenance components, including complete shield blanket replacement for breeding blankets. In-vessel components are activated by 14 MeV neutrons, so blanket maintenance requires remote handling equipment and tools able to handle heavy payloads of about 4 tons within a positioning accuracy of 2 mm under intense gamma radiation. To facilitate remote maintenance, blankets are segmented into 730 modules and rail-mounted vehicle remote maintenance was developed. According to the ITER R and D program, critical technology related to blanket maintenance was developed extensively through joint efforts of the Japan, EU, and U.S. home teams. This paper summarizes current blanket maintenance technology conducted by the Japan Home Team, including development of full-scale remote handling equipment and tools for blanket maintenance. (author)

  7. Development of divertor remote maintenance system

    Energy Technology Data Exchange (ETDEWEB)

    Takeda, Nobukazu; Oka, Kiyoshi; Akou, Kentaro; Takiguchi, Yuji [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-04-01

    The ITER divertor is categorized as a scheduled maintenance component because of extreme heat and particle loads it is exposed to by plasma. It is also highly activated by 14 MeV neutrons. Reliable remote handling equipment and tools are required for divertor maintenance under intense gamma radiation. To facilitate remote maintenance, the divertor is segmented into 60 cassettes, and each cassette weighing about 25 tons and maintained and replaced through four maintenance ports each 90 degrees. Divertor cassettes must be transported toroidally and radially for replacement through maintenance ports. Remote handling involving cassette movers and carriers for toroidal and radial transport has been developed. Under the ITER R and D program, technology critical to divertor cassette maintenance is being developed jointly by Japan, E.U., and U.S. home teams. This paper summarizes divertor remote maintenance design and the status of technology development by the Japan Home Team. (author)

  8. Development of blanket remote maintenance system

    Energy Technology Data Exchange (ETDEWEB)

    Kakudate, Satoshi; Nakahira, Masataka; Oka, Kiyoshi; Taguchi, Kou [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-04-01

    ITER in-vessel components such as blankets are scheduled maintenance components, including complete shield blanket replacement for breeding blankets. In-vessel components are activated by 14 MeV neutrons, so blanket maintenance requires remote handling equipment and tools able to handle heavy payloads of about 4 tons within a positioning accuracy of 2 mm under intense gamma radiation. To facilitate remote maintenance, blankets are segmented into 730 modules and rail-mounted vehicle remote maintenance was developed. According to the ITER R and D program, critical technology related to blanket maintenance was developed extensively through joint efforts of the Japan, EU, and U.S. home teams. This paper summarizes current blanket maintenance technology conducted by the Japan Home Team, including development of full-scale remote handling equipment and tools for blanket maintenance. (author)

  9. Development of divertor remote maintenance system

    International Nuclear Information System (INIS)

    Takeda, Nobukazu; Oka, Kiyoshi; Akou, Kentaro; Takiguchi, Yuji

    1998-01-01

    The ITER divertor is categorized as a scheduled maintenance component because of extreme heat and particle loads it is exposed to by plasma. It is also highly activated by 14 MeV neutrons. Reliable remote handling equipment and tools are required for divertor maintenance under intense gamma radiation. To facilitate remote maintenance, the divertor is segmented into 60 cassettes, and each cassette weighing about 25 tons and maintained and replaced through four maintenance ports each 90 degrees. Divertor cassettes must be transported toroidally and radially for replacement through maintenance ports. Remote handling involving cassette movers and carriers for toroidal and radial transport has been developed. Under the ITER R and D program, technology critical to divertor cassette maintenance is being developed jointly by Japan, E.U., and U.S. home teams. This paper summarizes divertor remote maintenance design and the status of technology development by the Japan Home Team. (author)

  10. Maintenance of Epithelial Stem Cells by Cbl Proteins

    Science.gov (United States)

    2012-09-01

    delivery of cytotoxic drugs conjugated to Trastuzumab. Trastuzumab-MCC-DM1 (T- DM1; DM1 is an anti-mitotic drug based on the Vinca alkaloid Maytansine) is...GL. HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1alpha (HIF-1alpha) synthesis : Novel mechanism for HIF-1-mediated vascular...factor. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 287, NO. 35, pp. 29442–29456, August 24, 2012 © 2012 by The American Society for Biochemistry and

  11. Maintenance of Epithelial Stem Cells by Cbl Proteins

    Science.gov (United States)

    2013-09-01

    corresponding authors). • Mohibi S, Gurumurthy CB, Nag A, Wang J, Mirza S, Mian Y, Quinn M, Katafiasz B, Eudy J, Pandey S, Guda C, Naramura M, Band H...MMTV-polyoma middle T transgenic mice. Cancer Res 67: 167–177. doi:10.1158/0008-5472.CAN-06-3782. 41. Li G, Robinson GW, Lesche R, Martinez-Diaz H

  12. Peace maintenance and political messages

    DEFF Research Database (Denmark)

    McAtackney, Laura

    2011-01-01

    The role of wall murals – as both territorial markers and as a means of maintaining and reinforcing identity – has been a frequent subject of study as a way of understanding the recent conflict in Northern Ireland, euphemistically known as ‘the Troubles’ (c. 1969–c. 1998). Whilst the heightened p......-essentializing their existence. It is suggested that there is a need to contextualize and consider the current and potential role of walls in the maintenance of peace and facilitating a ‘new’ Northern Ireland....

  13. Laboratory simulation of maintenance activity

    International Nuclear Information System (INIS)

    Kantowitz, B.H.

    1988-01-01

    Laboratory research in highly controlled settings can augment, but not replace, studies in plant or training center locations. A laboratory simulation involves abstraction of the human information processing and social interactions required in prototypical maintenance tasks. A variety of independent variables can be studied quickly, efficiently, and at relatively low cost. Sources of human error can be identified in terms of models of human perception, cognition, action, attention, and social/organizational processes. This paper discusses research in progress at the Battelle Human Performance Laboratory. Both theoretical aspects and practical implications are considered. Directions for future human factors research are indicated

  14. Mobile robotics for CANDU maintenance

    International Nuclear Information System (INIS)

    Lipsett, M.G.; Rody, K.H.

    1996-01-01

    Although robotics researchers have been promising that robotics would soon be performing tasks in hazardous environments, the reality has yet to live up to the hype. The presently available crop of robots suitable for deployment in industrial situations are remotely operated, requiring skilled users. This talk describes cases where mobile robots have been used successfully in CANDU stations, discusses the difficulties in using mobile robots for reactor maintenance, and provides near-term goals for achievable improvements in performance and usefulness. (author) 5 refs., 2 ills

  15. Handy help for maintenance workers

    International Nuclear Information System (INIS)

    Moore, T.

    1994-01-01

    Lightweight, low-cost, color-coded job cards packed with technical reference information and geared to specific types of maintenance and troubleshooting efficiency in nuclear as well as other types of power plants. Developed in conjunction with EPRI's counterpart in Japan, job card sets have been pilot-tested at two operating U.S. nuclear plants and are also being evaluated by Japanese utilities. In addition to their potential for utility customization, job cards could become a new line of EPRI information product that is used directly by the people who inspect and repair equipment in utility power plants

  16. Maintenance and Revitalization of Gallo

    DEFF Research Database (Denmark)

    Nolan, J. Shaun

    2018-01-01

    promotion organizations comprising teachers, linguists, enthusiasts, and activists are working hard at a local and regional level to ensure that there is a greater awareness of the existence of this language variety and to foster an appreciation of the importance of its maintenance and revitalization....... Whether these efforts will help Gallo become more used in eastern Brittany’s everyday sociolinguistic landscape remains to be seen. However, Gallo promotional organizations appear to be establishing the symbolic and cultural value of Gallo as part of Brittany’s languages, and are thereby solidifying...

  17. NIF Maintenance Plan March 2011

    Energy Technology Data Exchange (ETDEWEB)

    Van Wonterghem, Bruno M. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2014-01-30

    Ensuring the reliability of the NIF, including its support systems and utilities, is essential to ensuring the availability of the NIF in its support of laser operations. This document identifies the policies and procedures necessary to perform and support the maintenance of the NIF’s systems. These systems are all encompassing and include the facility, beampath, Line Replaceable Units (LRUs), Safety Interlock System (SIS), and diagnostics and utilities that create the environments within the beampath consisting of vacuum, argon, or clean dry air.

  18. Maintenance of Nuclear Power Plants

    International Nuclear Information System (INIS)

    Quintana, J. M.; Sanchez, J. T.

    2002-01-01

    With this article about the Maintenance in nuclear power plants we will try to give to see the importance of this kind of installations but the problems found by the clients and contractors to face it, and some possible solutions to improve it. It is necessary to understand this problem like something inner to the installation and must be considerate like a benefit for the same. Of course, there must be adequate Sevices Companies in direct relation with the installation that take the responsibility of assuming and understanding the correct fulfillment of the fixed milestones to get the optimal working of the whole plant systems. (Author)

  19. Proactive maintenance for mechanical systems

    CERN Document Server

    Fitch, EC

    1992-01-01

    Written by Dr. E.C. Fitch, the book contains over 340 double column pages which include 400 figures and tables, a comprehensive bibliography, and index. There is no root cause of mechanical failure, known to the author, that has been ignored or left out. Nowhere in the world is this information put together in such a concise and comprehensive manner, and the book will serve as a reference and guide to designers, practising engineers, maintenance technicians, plant managers and operators who must design, maintain and operate fluid-dependent mechanical systems.

  20. [Maintenance care for dental implant].

    Science.gov (United States)

    Kamoi, K

    1989-10-01

    Dental implant has tried at the early stage in 19th century recovering an oral function and esthetics. Technological revolutions in biochemical and new materials have developed on the remarkable change in the dental implants, nowadays we call the three generation therapy for dental implantology. There are many kinds of methods and techniques in dental implants, however a lot of troublesome complication on the process of surgical phase, construction of prothodontics and prognosis of maintenance care. In the proceedings of this symposium, I would like to propose you how to manage the maintenance care for various kind of dental implants through the methodology and case presentations. Tendenay and future for dental implants The current outlook of dental implant has increasing supply and demand not only dentists but also patients. According to Japanese Welfare Ministry's report in 1987, average missing teeth over sixty years old generations are approximately 42% in accordance with NIDR (U.S.A.) research. They are missed on ten over teeth in full 28th teeth dentitions owing to dental caries and periodontal diseases. Generally speaking, latent implant patients are occupied on the same possibility of needs for dental implants both Japan and U.S.A. Management of maintenance care The patients hardly recognized the importance of plaque control for the maintenance care in the intraoral condition after implantation. Dentists and dental staffs must be instruct patients for importance of plaque removal and control, because they already had forgotten the habit of teeth cleaning, especially in the edenturous conditions. 1) Concept of establishment in oral hygiene. Motivation and instruction for patients include very important factors in dental implants as well as in periodontal diseases. Patients who could not achieve on good oral hygiene levels obtained no good results in the long term observations. To establish good oral hygiene are how to control supra plaque surrounding tissues

  1. Ontology Maintenance using Textual Analysis

    Directory of Open Access Journals (Sweden)

    Yassine Gargouri

    2003-10-01

    Full Text Available Ontologies are continuously confronted to evolution problem. Due to the complexity of the changes to be made, a maintenance process, at least a semi-automatic one, is more and more necessary to facilitate this task and to ensure its reliability. In this paper, we propose a maintenance ontology model for a domain, whose originality is to be language independent and based on a sequence of text processing in order to extract highly related terms from corpus. Initially, we deploy the document classification technique using GRAMEXCO to generate classes of texts segments having a similar information type and identify their shared lexicon, agreed as highly related to a unique topic. This technique allows a first general and robust exploration of the corpus. Further, we apply the Latent Semantic Indexing method to extract from this shared lexicon, the most associated terms that has to be seriously considered by an expert to eventually confirm their relevance and thus updating the current ontology. Finally, we show how the complementarity between these two techniques, based on cognitive foundation, constitutes a powerful refinement process.

  2. The ITER remote maintenance system

    International Nuclear Information System (INIS)

    Tesini, A.; Palmer, J.

    2007-01-01

    ITER is a joint international research and development project that aims to demonstrate the scientific and technological feasibility of fusion power. As soon as the plasma operation begins using tritium, the replacement of the vacuum vessel internal components will need to be done with remote handling techniques. To accomplish these operations ITER has equipped itself with a Remote Maintenance System; this includes the Remote Handling equipment set and the Hot Cell facility. Both need to work in a cooperative way, with the aim of minimizing the machine shutdown periods and to maximize the machine availability. The ITER Remote Handling equipment set is required to be available, robust, reliable and retrievable. The machine components, to be remotely handle-able, are required to be designed simply so as to ease their maintenance. The baseline ITER Remote Handling equipment is described. The ITER Hot Cell Facility is required to provide a controlled and shielded area for the execution of repair operations (carried out using dedicated remote handling equipment) on those activated components which need to be returned to service, inside the vacuum vessel. The Hot Cell provides also the equipment and space for the processing and temporary storage of the operational and decommissioning radwaste. A conceptual ITER Hot Cell Facility is described. (orig.)

  3. Maintenance of Certification®, Maintenance of Public Trust

    Science.gov (United States)

    Chung, Kevin C.; Clapham, Philip J.; Lalonde, Donald H.

    2010-01-01

    The Maintenance of Certification® (MOC) program has been well received by many physicians, but faced significant opposition from others, who complain that it is overly tedious, costly, and irrelevant to their practice. This article offers a consolidated and concise history of the MOC program and a summary of what plastic surgeons need to know to successfully complete the American Board of Plastic Surgery’s (ABPS) own MOC requirements. The authors have justified each step of the ABPS’s MOC process in terms of how it improves the quality of care delivered to Plastic Surgery patients. Finally, a summary of research is presented that demonstrates both that the public supports the MOC process for all physicians and that continuing education and formal assessment and improvement initiatives have been linked in multiple studies to a better and more evidence-based medical practice. PMID:21285803

  4. Estimating Maintenance Cost for Web Applications

    Directory of Open Access Journals (Sweden)

    Ion IVAN

    2016-01-01

    Full Text Available The current paper tackles the issue of determining a method for estimating maintenance costs for web applications. The current state of research in the field of web application maintenance is summarized and leading theories and results are highlighted. The cost of web maintenance is determined by the number of man-hours invested in maintenance tasks. Web maintenance tasks are categorized into content maintenance and technical maintenance. Research is centered on analyzing technical maintenance tasks. The research hypothesis is formulated on the assumption that the number of man-hours invested in maintenance tasks can be assessed based on the web application’s user interaction level, complexity and content update effort. Data regarding the costs of maintenance tasks is collected from 24 maintenance projects implemented by a web development company that tackles a wide area of web applications. Homogeneity and diversity of collected data is submitted for debate by presenting a sample of the data and depicting the overall size and comprehensive nature of the entire dataset. A set of metrics dedicated to estimating maintenance costs in web applications is defined based on conclusions formulated by analyzing the collected data and the theories and practices dominating the current state of research. Metrics are validated with regards to the initial research hypothesis. Research hypothesis are validated and conclusions are formulated on the topic of estimating the maintenance cost of web applications. The limits of the research process which represented the basis for the current paper are enunciated. Future research topics are submitted for debate.

  5. Protein Hydrolysates/Peptides in Animal Nutrition

    Science.gov (United States)

    McCalla, Jeff; Waugh, Terry; Lohry, Eric

    The use of protein hydrolysates as an important nutrient for growth and maintenance has been increasing in animal nutrition. Although animal proteins and protein hydrolysates are widely used however, recently vegetable protein hydrolysates are gaining importance. This chapter reviews the use of protein hydrolysates developed by enzyme hydrolysis and by solid state fermentation process in animal nutrition especially for piglets and compares it with the standard products such as plasma and fishmeal.

  6. Maintenance evaluation using risk based criteria

    International Nuclear Information System (INIS)

    Torres Valle, A.

    1996-01-01

    The maintenance evaluation is currently performed by using economic and, in some case, technical equipment failure criteria, however this is done to a specific equipment level. In general, when statistics are used the analysis for maintenance optimization are made isolated and whit a post mortem character; The integration provided by mean of Probabilistic Safety assessment (PSA) together with the possibilities of its applications, allow for evaluation of maintenance on the basis of broader scope criteria in regard to those traditionally used. The evaluate maintenance using risk based criteria, is necessary to follow a dynamic and systematic approach, in studying the maintenance strategy, to allow for updating the initial probabilistic models, for including operational changes that often take place during operation of complex facilities. This paper proposes a dynamic evaluation system of maintenance task. The system is illustrated by means of a practical example

  7. Second Line of Defense: Electronic Maintenance Reports, Local Maintenance Provider User Guide, Rev. 3

    Energy Technology Data Exchange (ETDEWEB)

    Leigh, Richard J.

    2012-09-01

    The Electronic Maintenance Report forms allow Local Maintenance Providers (LMP) and other program staff to enter maintenance information into a simple and secure system. This document describes the features and information required to complete the Maintenance Report forms. It is expected that all Corrective Maintenance Reports from LMPs will be submitted electronically into the SLD Portal. As an exception (e.g., when access to the SLD Portal is unavailable), Maintenance Reports can be submitted via a secure Adobe PDF form available through the Sustainability Manager assigned to each country.

  8. Simulated maintenance a virtual reality

    International Nuclear Information System (INIS)

    Lirvall, P.

    1995-01-01

    The article describes potential applications of personal computer-based virtual reality software. The applications are being investigated by Atomic Energy of Canada Limited's (AECL) Chalk River Laboratories for the Canadian deuterium-uranium (Candu) reactor. Objectives include: (1) reduction of outage duration and improved safety, (2) cost-effective and safe maintenance of equipment, (3) reduction of exposure times and identification of overexposure situations, (4) cost-effective training in a virtual control room simulator, (5) human factors evaluation of design interface, and (6) visualization of conceptual and detailed designs of critical nuclear field environments. A demonstration model of a typical reactor control room, the use of virtual reality in outage planning, and safety issues are outlined

  9. Preventative maintenance of straddle carriers

    Directory of Open Access Journals (Sweden)

    Si Li

    2015-04-01

    Objectives: The purpose of this industry-driven study is to model preventative maintenance (PM influences on the operational effectiveness of straddle carriers. Method: The study employs historical data consisting of 21 273 work orders covering a 27-month period. Two models are developed, both of which forecast influences of PM regimes for different types of carrier. Results: The findings of the study suggest that the reliability of the straddle fleet decreases with increased intervals of PM services. The study also finds that three factors – namely resources, number of new straddles, and the number of new lifting work centres – influence the performances of straddles. Conclusion: The authors argue that this collaborative research exercise makes a significant contribution to existing supply chain management literature, particularly in the area of operations efficiency. The study also serves as an avenue to enhance relevant management practice.

  10. The divertor remote maintenance project

    International Nuclear Information System (INIS)

    Maisonnier, D.; Martin, E.; Akou, K.

    2001-01-01

    Remote replacement of the ITER divertor will be required several times during the life of ITER. To facilitate its regular exchange, the divertor is assembled in the ITER vacuum vessel from 60 cassettes. Radial movers transport each cassette along radial rails through the handling ports and into the vessel where a toroidal mover lifts and transports the cassette around a pair of toroidal rails. Once at its final position the cassette is locked to the toroidal rails and is accurately aligned in both poloidal and toroidal directions. A further requirement on the divertor is to minimise the amount of activated waste to be sent to a repository. To this end the cassettes have been designed to allow the remote replacement, in a hot cell, of their plasma facing components. The paper describes the two facilities built at ENEA Brasimone, Italy, whose aim is to demonstrate the reliable remote maintenance of the divertor cassettes. (author)

  11. The divertor remote maintenance project

    International Nuclear Information System (INIS)

    Maisonnier, D.; Martin, E.; Akou, K.

    1999-01-01

    Remote replacement of the ITER divertor will be required several times during the life of ITER. To facilitate its regular exchange, the divertor is assembled in the ITER vacuum vessel from 60 cassettes. Radial movers transport each cassette along radial rails through the handling ports and into the vessel where a toroidal mover lifts and transports the cassette around a pair of toroidal rails. Once at its final position the cassette is locked to the toroidal rails and is accurately aligned in both poloidal and toroidal directions. A further requirement on the divertor is to minimise the amount of activated waste to be sent to a repository. To this end the cassettes have been designed to allow the remote replacement, in a hot cell, of their plasma facing components. The paper describes the two facilities built at ENEA Brasimone, Italy, whose aim is to demonstrate the reliable remote maintenance of the divertor cassettes. (author)

  12. Adherence to Dietary Recommendations in Maintenance Phase Kidney Transplant Patients.

    Science.gov (United States)

    Ichimaru, N; Nakazawa, S; Yamanaka, K; Kakuta, Y; Abe, T; Kaimori, J-Y; Imamura, R; Nonomura, N; Takahara, S

    2016-04-01

    Current adherence to dietary recommendations for chronic kidney disease was evaluated in kidney transplant patients in the maintenance phase. A total of 268 maintenance phase kidney transplant patients were included in the study. Estimated daily intakes of oral protein and salt were calculated from 24-h urinary excretion of nitrogen and sodium, respectively. Dietary recommendations for chronic kidney disease, as issued in 2014 by the Japanese Society of Nephrology, were used as the basis for assessing diet. The study included 114 female patients and 154 male patients. The mean age, posttransplantation years, body mass index, estimated glomerular filtration rate, and 24-h urinary excretion of protein were 56.3 years, 11.2 years, 22.0 kg/m(2), 42.6 mL/min/1.73 m(2), and 321 mg/d, respectively. Estimated daily protein and salt intakes were 0.98 ± 0.26 g/kg/d and 9.3 ± 3.9 g/d. Only 47 patients (17.5%) in the case of salt intake and 105 patients (39.2%) in the case of protein intake were within reference values. The 24-h urinary protein excretion of the daily salt intake-adherent group (adherence rate to dietary recommendations for chronic kidney disease in kidney transplant patients was low. The 24-h urinary protein excretion of the daily salt intake-adherent group was significantly less than that of the nonadherent group. Dietary therapy for these patients may have the potential to improve kidney graft function and survival. Copyright © 2016 Elsevier Inc. All rights reserved.

  13. Machinery prognostics and prognosis oriented maintenance management

    CERN Document Server

    Yan, Jihong

    2014-01-01

    This book gives a complete presentatin of the basic essentials of machinery prognostics and prognosis oriented maintenance management, and takes a look at the cutting-edge discipline of intelligent failure prognosis technologies for condition-based maintenance.  Latest research results and application methods are introduced for signal processing, reliability moelling, deterioration evaluation, residual life prediction and maintenance-optimization as well as applications of these methods.

  14. Software Maintenance Management Evaluation and Continuous Improvement

    CERN Document Server

    April, Alain

    2008-01-01

    This book explores the domain of software maintenance management and provides road maps for improving software maintenance organizations. It describes full maintenance maturity models organized by levels 1, 2, and 3, which allow for benchmarking and continuous improvement paths. Goals for each key practice area are also provided, and the model presented is fully aligned with the architecture and framework of software development maturity models of CMMI and ISO 15504. It is complete with case studies, figures, tables, and graphs.

  15. Trends and Perspective in Industrial Maintenance Management

    DEFF Research Database (Denmark)

    Luxhoj, James T.; Thorsteinsson, Uffe; Riis, Jens Ove

    1997-01-01

    With increased global competition for manufacturing, many companies are seeking ways to gain competitative advanges with respect to cost, service, quality and on-time deliveries. The role that effective maintenance management plays in contributing to overall organizational productivity has received...... increased attention. Trends and perspective in industrial maintenance are presented. The result of benchmarking studies from Scandinavia and United States are also presented and compared. Case studies that examine maintenance methods, knowledge, organization and information systems in three Danish...

  16. Performance Improvement through Implementation of Lean Maintenance

    OpenAIRE

    S. Kolanjiappan; Dr. K. Maran

    2011-01-01

    Lean Maintenance is a relatively new term, invented in the last decade of the twentieth century, but the principles are well established in Total Productive Maintenance (TPM). Lean Maintenance—taking its lead from Lean Manufacturing—applies some new techniques to TPM concepts to render a more structured implementation path. Tracing its roots back to Henry Ford with modern refinements born in Japanese manufacturing, specifically the Toyota Production System (TPS). Lean seeks to eliminate a...

  17. The maintenance management for lean organization

    OpenAIRE

    Daniel D. GEORGESCU

    2010-01-01

    The goal of lean maintenance approach is to maintain organization international competitiveness. In the spirit of continuously improvement, in the paper it is shown how by adjusting some Lean manufacturing specific techniques for Total Productive Maintenance (TPM) system, was born new maintenance system named Lean TPM. This is a systemic approach with three techniques, which don’t exclude specific techniques of TPM pillars, but complete them: the 7Ss as a critical first step in any improvemen...

  18. PFP MICON maintenance manual. Revision 1

    International Nuclear Information System (INIS)

    Silvan, G.R.

    1995-01-01

    This manual covers the use of maintenance displays, maintenance procedures, system alarms and common system failures. This manual is intended to supplement the MICON maintenance training not replace it. It also assumes that the user is familiar with the normal operation of the MICON A/S system. The MICON system is a distributed control computer and, among other things, controls the HVAC system for the Plutonium Finishing Plant

  19. On-Line Maintenance Methodology Development

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Hyo Won; Kim, Jae Ho; Jae, Moo Sung [Hanyang University, Seoul (Korea, Republic of)

    2012-05-15

    Most of domestic maintenance activities for nuclear power plants are performed while overhaul. Therefore, On-Line Maintenance (OLM) is one of the proper risks informed application techniques for diffusing maintenance burden during overhaul with safety of the plant is secured. The NUMARC 93-01 (Rev.3) presents the OLM state of the art and it provides methodology. This study adopts NUMARC 93-01 (Rev.3) and present OLM. The reference component is Emergency Diesel Generator (EDG) of Ulchin 3, 4

  20. Tandem mirror next step: remote maintenance

    International Nuclear Information System (INIS)

    Doggett, J.N.; Damm, C.C.; Hanson, C.L.

    1980-01-01

    This study of the next proposed experiment in the Mirror Fusion Program, the Tandem Mirror Next Step (TMNS), has included serious consideration of the maintenance requirements of such a large source of high energy neutrons with its attendant throughput of tritium. Although maintenance will be costly in time and money, our conclusion is that with careful attention to a design for maintenance plan such a device can be reliably operated

  1. Inspection of licensee - Maintenance programme and activities

    International Nuclear Information System (INIS)

    2013-01-01

    An effective maintenance programme is critical to sustained safe and reliable operation of nuclear power plants. The Working Group on Inspection Practices (WGIP) concluded that when a licensee has an effective maintenance programme, the overall operating safety of the plant is improved and the protection of public health and safety enhanced. All Regulatory Bodies (RB) consider maintenance to be an important area for oversight. Although a variety of inspection practices are being used; RB are actively monitoring licensee performance. Specifically the following conclusions were reached and commendable practices identified: - Maintenance oversight by regulators appears to be in a stable continuous improvement state. Most regulators are executing inspection oversight based on an existing regulatory framework. - The performance of a licensee's maintenance programme is recognized as important part of maintaining nuclear safety. The result of the maintenance program assessment is included in the overall performance assessment of a license. - Maintenance inspection activities are recognized as an important part of the regulatory oversight process. Inspection activities are based on the safety significance and nature of work being performed by the licensee. - The effectiveness of the maintenance inspection activities is recognized to rely on properly qualified inspectors; who are adequately supported by specialists. Training and qualification of inspectors should be based on how the RB reviews and inspects licensee maintenance programmes. - Reporting requirements are identified to provide information on the licensees maintenance programme, and to help guide inspection activities. - Performance Indicators are recognized as a useful tool for helping focus regulatory activities. Basic PI are identified and tracked by the RB, and use of PI by the licensee is monitored. - Inspections are designed to confirm that the licensee is planning and scheduling maintenance with due

  2. Pavement maintenance scheduling using genetic algorithms

    OpenAIRE

    Yang, Chao; Remenyte-Prescott, Rasa; Andrews, John D.

    2015-01-01

    This paper presents a new pavement management system (PMS) to achieve the optimal pavement maintenance and rehabilitation (M&R) strategy for a highway network using genetic algorithms (GAs). Optimal M&R strategy is a set of pavement activities that both minimise the maintenance cost of a highway network and maximise the pavement condition of the road sections on the network during a certain planning period. NSGA-II, a multi-objective GA, is employed to perform pavement maintenance optimisatio...

  3. Advantages of condition-based maintenance approach

    International Nuclear Information System (INIS)

    Bareiss, J.; Roos, E.; Jovanovic, A.; Perunicic, M.; Balos, D.

    2004-01-01

    Pilot applications in EnBW steam boiler components and high-pressure pipe systems in the context of the European research project RIMAP (''Risk Based Inspection and Maintenance Procedures for European Industry'') showed that application of a risk-based maintenance strategy (e.g. RIMAP) will make the decision process more transparent and enable better condition-oriented maintenance. With regard to life management of the systems (availability, product quality), this appears to be more important than than the aspect of cost reduction in the maintenance sector, although the latter may not be unwelcome. (orig.) [de

  4. Remote maintenance in nuclear fuel reprocessing

    International Nuclear Information System (INIS)

    Herndon, J.N.

    1985-01-01

    Remote maintenance techniques applied in large-scale nuclear fuel reprocessing plants are reviewed with particular attention to the three major maintenance philosophy groupings: contact, remote crane canyon, and remote/contact. Examples are given, and the relative success of each type is discussed. Probable future directions for large-scale reprocessing plant maintenance are described along with advanced manipulation systems for application in the plants. The remote maintenance development program within the Consolidated Fuel Reprocessing Program at the Oak Ridge National Laboratory is also described. 19 refs., 19 figs

  5. Contracting, An Alarming Trend in Aviation Maintenance

    National Research Council Canada - National Science Library

    Brooke, J

    1998-01-01

    .... Aviation operational and maintenance units struggle to balance peacetime requirements for general military and technical training, organization and installation support, training and operational...

  6. Fleet Aviation Maintenance Organic Support (FAMOS) Laboratory

    Data.gov (United States)

    Federal Laboratory Consortium — Purpose:The Fleet Aviation Maintenance Organic Support (FAMOS) Laboratory at the Naval Air Warfare Center Aircraft Division, Lakehurst, NJ provides rapid engineering...

  7. Task oriented evaluation system for maintenance robots

    International Nuclear Information System (INIS)

    Asame, Hajime; Endo, Isao; Kotosaka, Shin-ya; Takata, Shozo; Hiraoka, Hiroyuki; Kohda, Takehisa; Matsumoto, Akihiro; Yamagishi, Kiichiro.

    1994-01-01

    The adaptability evaluation of maintenance robots to autonomous plants has been discussed. In this paper, a new concept of autonomous plant with maintenance robots are introduced, and a framework of autonomous maintenance system is proposed. Then, task-oriented evaluation of robot arms is discussed for evaluating their adaptability to maintenance tasks, and a new criterion called operability is proposed for adaptability evaluation. The task-oriented evaluation system is implemented and applied to structural design of robot arms. Using genetic algorithm, an optimal structure adaptable to a pump disassembly task is obtained. (author)

  8. Conceptualization of an intellectual maintenance management system

    International Nuclear Information System (INIS)

    Suda, Kazunori; Yonekawa, Tsuyoshi; Yoshikawa, Shinji; Ozawa, Kenji

    1997-04-01

    It is studied in many research institute to enhance availability and safety of nuclear power plants operation and maintenance. On this account, development of autonomous plants has been carried out to replace the role of operators with artificial intelligence and autonomous robots. We have been developing an intellectual maintenance management system since 1994. As the first step, concept of an intellectual maintenance management system was constructed. The intellectual maintenance managerial system is in charge of maintenance function of an autonomous plant. The intellectual maintenance managerial system has three functions which is monitoring state and judging abnormal machine and deciding maintenance plan by autonomy. This system has an important role of indication and communication of the result to an autonomous operation system and autonomous robot. In this examination, we adopted the distributed and cooperative system technique by multi-agent of AI technology and examined a method to enforce problem solving by cooperation of sensor and actuator. In this report, we examined trouble detection and troubleshooting evaluation and maintenance plan decision function by the distributed and cooperative system technology, the distributed and cooperative system communication-function that these information releases functions was taken on. In conceptualization of the intellectual maintenance managerial system, we clarified of major functions to constitute this system and relation between autonomous operation system and autonomous robots. We clarified the information exchange scheme between this system and an outside system furthermore. In future, we will prototype each function and inspect the total system. (author)

  9. [Development of Hospital Equipment Maintenance Information System].

    Science.gov (United States)

    Zhou, Zhixin

    2015-11-01

    Hospital equipment maintenance information system plays an important role in improving medical treatment quality and efficiency. By requirement analysis of hospital equipment maintenance, the system function diagram is drawed. According to analysis of input and output data, tables and reports in connection with equipment maintenance process, relationships between entity and attribute is found out, and E-R diagram is drawed and relational database table is established. Software development should meet actual process requirement of maintenance and have a friendly user interface and flexible operation. The software can analyze failure cause by statistical analysis.

  10. New activities in nuclear power plants maintenance

    International Nuclear Information System (INIS)

    Mico, F.; Specht, J.

    2006-01-01

    This article addresses the consequences of the progressive deregulation of the electricity market of several key countries on the maintenance practices and techniques face to the growing pressures on O and M costs. The responses of the nuclear maintenance industry to make maintenance activities more efficient maintaining if not enhancing the safety of NPP's are briefly described. The article also analyses the potential impact of shortage of experience maintenance personnel to serve the NPP's in the future as some countries are already experiencing. (Author)

  11. MOV predictive maintenance program at Darlington NGS

    International Nuclear Information System (INIS)

    Morrison, J.F.

    1992-01-01

    This paper details the Motor Operated Valve (MOV) Predictive Maintenance program at Darlington Nuclear Generating Station. The program encompasses the use of diagnostics tooling in conjunction with more standard maintenance techniques, with the goal of improving performance of MOV's. Problems encountered and solutions developed during the first two phases of this program are presented, along with proposed actions for the final trending phase of the program. This paper also touches on the preventive and corrective maintenance aspects of an overall MOV maintenance program. 6 refs., 6 tabs., 6 figs

  12. Remote maintenance for fusion experimental reactor

    International Nuclear Information System (INIS)

    Koizumi, Koichi; Takeda, Nobukazu

    2000-01-01

    Here was introduced on maintenance of reactor core portion operated by remote control among maintenance of the International Thermonuclear Experimental Reactor (ITER) begun on its design since 1988 under international cooperation of U.S.A., Europe, Russia and Japan. Every appliances constructing the reactor core portion is necessary to carry out all of their inspection and maintenance by using remote controlled apparatus because of their radiation due to neutron generated by DT combustion of plasma. For engineering design activity (EDA) in ITER, not only design and development of the remote control appliances but also design under consideration of remote maintenance for from structural design of maintained objective appliances to access method to appliances, transportation and preservation method of radiated matters, and out-reactor maintenance in a hot cell, is now under progress. Here were also reported on basic concept on maintenance and conservation of ITER, maintenance design of diverter and blanket with high maintenance frequency and present state on development of maintenance appliances. (G.K.)

  13. A proactive condition-based maintenance strategy with both perfect and imperfect maintenance actions

    International Nuclear Information System (INIS)

    Do, Phuc; Voisin, Alexandre; Levrat, Eric; Iung, Benoit

    2015-01-01

    This paper deals with a proactive condition-based maintenance (CBM) considering both perfect and imperfect maintenance actions for a deteriorating system. Perfect maintenance actions restore completely the system to the ‘as good as new’ state. Their related cost are however often high. The first objective of the paper is to investigate the impacts of imperfect maintenance actions. In fact, both positive and negative impacts are considered. Positive impact means that the imperfect maintenance cost is usually low. Negative impact implies that (i) the imperfect maintenance restores a system to a state between good-as-new and bad-as-old and (ii) each imperfect preventive action may accelerate the speed of the system's deterioration process. The second objective of the paper is to propose an adaptive maintenance policy which can help to select optimally maintenance actions (perfect or imperfect actions), if needed, at each inspection time. Moreover, the time interval between two successive inspection points is determined according to a remaining useful life (RUL) based-inspection policy. To illustrate the use of the proposed maintenance policy, a numerical example finally is introduced. - Highlights: • A new imperfect maintenance model for deterioration system is proposed. • Both positive and negative impacts of an imperfect maintenance action are investigated. • An adaptive condition-based maintenance policy is introduced. • The optimal number of useful imperfect maintenance actions for each life cycle is optimally provided

  14. North American nuclear maintenance best practices compared to Japanese utility maintenance practices

    International Nuclear Information System (INIS)

    Harazim, M.L.; Ferguson, B.J.

    2003-01-01

    The purpose of this paper is to compare the best practices in North America concerning Preventive Maintenance, Predictive Maintenance and Reliability Centered Maintenance (RCM) or Preventive Maintenance Optimization (PMO) with the time-directed maintenance philosophies utilized by Japanese utilities, and how the Japanese utilities are considering (and some have begun) adopting North American ways of doing business. Preventive Maintenance Living Programs are also discussed, along with the Institute of Nuclear Power Operations AP-913 process, Equipment Reliability Process. Also, a cost benefit analysis will be demonstrated showing how major cost savings can be achieved by shifting from a predominantly time-directed maintenance philosophy to a predominantly predictive strategy utilizing vibration analysis, lubricating oil analysis, thermography and other predictive technologies to better utilize resources, reduce spare part consumption and to minimize maintenance induced errors. As a result of the strides made while incorporating these new philosophies, we no longer advocate tearing apart perfectly good equipment. (author)

  15. Lean Maintenance Applied to Improve Maintenance Efficiency in Thermoelectric Power Plants

    OpenAIRE

    Orlando Duran; Andrea Capaldo; Paulo Andrés Duran Acevedo

    2017-01-01

    Thermoelectric power plants consist of a set of critical equipment that require high levels of availability and reliability. Due to this, maintenance of these physical assets is gaining momentum in industry. Maintenance is considered as an activity that contributes to improving the availability, efficiency and productivity of each piece of equipment. Several techniques have been used to achieve greater efficiencies in maintenance, among which we can find the lean maintenance philosophy. Despi...

  16. 75 FR 4099 - Maintenance Wage Rate Wage Recommendation and Maintenance Wage Survey; Report of Additional...

    Science.gov (United States)

    2010-01-26

    ... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5376-N-06] Maintenance Wage Rate Wage Recommendation and Maintenance Wage Survey; Report of Additional Classification and Wage Rate AGENCY: Office of... proposal. The information is used by HUD to determine or adopt prevailing wage rates for maintenance...

  17. 75 FR 37457 - Maintenance Wage Rate Wage Recommendation and Maintenance Wage Survey; Report of Additional...

    Science.gov (United States)

    2010-06-29

    ... DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT [Docket No. FR-5376-N-54] Maintenance Wage Rate Wage Recommendation and Maintenance Wage Survey; Report of Additional Classification and Wage Rate AGENCY: Office of... proposal. The information is used by HUD to determine or adopt prevailing wage rates for maintenance...

  18. Condition-based maintenance for complex systems : Coordinating maintenance and logistics planning for the process industries

    NARCIS (Netherlands)

    Olde Keizer, Minou Catharina Anselma

    2016-01-01

    Maintenance planning in the process industries is extremely complex for various reasons. Plants often run nonstop, allowing little time for performing preventive maintenance. Failures should be prevented, however, as they can lead to system downtime and high losses of revenue. Corrective maintenance

  19. Equipment Maintenance management support system based on statistical analysis of maintenance history data

    International Nuclear Information System (INIS)

    Shimizu, S.; Ando, Y.; Morioka, T.

    1990-01-01

    Plant maintenance is recently becoming important with the increase in the number of nuclear power stations and in plant operating time. Various kinds of requirements for plant maintenance, such as countermeasures for equipment degradation and saving maintenance costs while keeping up plant reliability and productivity, are proposed. For this purpose, plant maintenance programs should be improved based on equipment reliability estimated by field data. In order to meet these requirements, it is planned to develop an equipment maintenance management support system for nuclear power plants based on statistical analysis of equipment maintenance history data. The large difference between this proposed new method and current similar methods is to evaluate not only failure data but maintenance data, which includes normal termination data and some degree of degradation or functional disorder data for equipment and parts. So, it is possible to utilize these field data for improving maintenance schedules and to evaluate actual equipment and parts reliability under the current maintenance schedule. In the present paper, the authors show the objectives of this system, an outline of this system and its functions, and the basic technique for collecting and managing of maintenance history data on statistical analysis. It is shown, from the results of feasibility tests using simulation data of maintenance history, that this system has the ability to provide useful information for maintenance and the design enhancement

  20. Predicting maintenance of attendance at walking groups: testing constructs from three leading maintenance theories.

    Science.gov (United States)

    Kassavou, Aikaterini; Turner, Andrew; Hamborg, Thomas; French, David P

    2014-07-01

    Little is known about the processes and factors that account for maintenance, with several theories existing that have not been subject to many empirical tests. The aim of this study was to test how well theoretical constructs derived from the Health Action Process Approach, Rothman's theory of maintenance, and Verplanken's approach to habitual behavior predicted maintenance of attendance at walking groups. 114 participants, who had already attended walking groups in the community for at least 3 months, completed a questionnaire assessing theoretical constructs regarding maintenance. An objective assessment of attendance over the subsequent 3 months was gained. Multilevel modeling was used to predict maintenance, controlling for clustering within walking groups. Recovery self-efficacy predicted maintenance, even after accounting for clustering. Satisfaction with social outcomes, satisfaction with health outcomes, and overall satisfaction predicted maintenance, but only satisfaction with health outcomes significantly predicted maintenance after accounting for clustering. Self-reported habitual behavior did not predict maintenance despite mean previous attendance being 20.7 months. Recovery self-efficacy, and satisfaction with health outcomes of walking group attendance appeared to be important for objectively measured maintenance, whereas self-reported habit appeared not to be important for maintenance at walking groups. The findings suggest that there is a need for intervention studies to boost recovery self-efficacy and satisfaction with outcomes of walking group attendance, to assess impact on maintenance.

  1. Grounds Maintenance Equipment and Procedures. Building Maintenance. Module VI. Instructor's Guide.

    Science.gov (United States)

    Crank, Virgil

    This curriculum guide, one of six modules keyed to the building maintenance competency profile developed by industry and education professionals, provides materials for two units on grounds maintenance equipment and procedures. The first unit deals with grounds maintenance equipment and includes the following lessons: safety; changing oil/filter…

  2. Relationship Maintenance on Facebook: Development of a Measure, Relationship to General Maintenance, and Relationship Satisfaction

    Science.gov (United States)

    Dainton, Marianne

    2013-01-01

    Previous research indicates that the primary reason college students use Facebook is for relationship maintenance. The present study sought to determine the relationship between Facebook maintenance and general maintenance efforts in college student romantic relationships, as well as the impacts of such behaviors on the relationship. Survey data…

  3. Croatian Language Maintenance in Canada

    Directory of Open Access Journals (Sweden)

    Ivana Petrović

    2017-01-01

    Full Text Available Although the topic of language maintenance has received considerable attention from linguists around the world, there are still many aspects of this language-contact phenomenon that could be examined further. This paper aims to contribute to the existing body of knowledge by exploring the state of Croatian as a heritage language in Canada. The aim of the paper is two-fold. The first is to describe the demographic characteristics of the Croatian community by investigating the number of people of Croatian descent and the number of Croatian speakers in Canada. The second, and more specific, aim of the paper is to provide an account of the state of Croatian as a minority language and examine the extent of language maintenance in the community. To accomplish the first objective, Canadian census data (1996, 2001, 2006, and 2011 was analyzed, with special focus on linguistic census data (number and age of Croatian speakers in Canada, mother tongue of people of Croatian descent, language most used at home, etc.. To accomplish the second objective, census data was supplemented with data from a questionnaire-based survey completed by members of the Croatian community in Toronto. The survey was completed by 220 participants; 110 first-generation Croatian Canadians and 110 second-generation Croatian Canadians. Two versions of the questionnaire were designed, one for first-generation participants and the other for second-generation participants. The great majority of items in the two versions were identical; each version contained questions about demographic characteristics, language use in everyday life, and self-perceived language proficiency in English and Croatian. The majority of questions were of a closed type (multiple-choice questions and rating scales, but there were also some open-ended questions, so as to give participants the opportunity to express their viewpoint or comment on certain issues. Questions were written in both Croatian and English

  4. Simulation based comparison of predictive maintenance policies

    NARCIS (Netherlands)

    Tinga, Tiedo; Janssen, R.H.P.; Steenbergen, R.; van Gelder, P.

    2013-01-01

    When an asset is operated in variable conditions, its operational efficiency can be improved significantly when the maintenance is performed in a dynamic manner. This means that variations in usage and operating environment are taken into account when deciding on the length of the maintenance

  5. Void Fraction Instrument operation and maintenance manual

    International Nuclear Information System (INIS)

    Borgonovi, G.; Stokes, T.I.; Pearce, K.L.; Martin, J.D.; Gimera, M.; Graves, D.B.

    1994-09-01

    This Operations and Maintenance Manual (O ampersand MM) addresses riser installation, equipment and personnel hazards, operating instructions, calibration, maintenance, removal, and other pertinent information necessary to safely operate and store the Void Fraction Instrument. Final decontamination and decommissioning of the Void Fraction Instrument are not covered in this document

  6. Maintenance Personnel Performance Simulation (MAPPS) model

    International Nuclear Information System (INIS)

    Siegel, A.I.; Bartter, W.D.; Wolf, J.J.; Knee, H.E.; Haas, P.M.

    1984-01-01

    A stochastic computer model for simulating the actions and behavior of nuclear power plant maintenance personnel is described. The model considers personnel, environmental, and motivational variables to yield predictions of maintenance performance quality and time to perform. The mode has been fully developed and sensitivity tested. Additional evaluation of the model is now taking place

  7. Principles of machine operation and maintenance

    CERN Document Server

    Jeffrey, Dick

    2013-01-01

    This book explains how rotating machinery works, and the role of the maintenance engineer in ensuring its proper operation. Stress is laid on the need for the trainee engineer to develop skills in diagnosis and troubleshooting as well as practicalexpertise in maintenance procedures.

  8. Maintenance and Repair of Concrete Structures

    NARCIS (Netherlands)

    Bijen, J.M.J.M.

    1989-01-01

    In 1987 and 1988 a series of articles was published in the Dutchjournal "Cement" about maintenance and repair of concrete structures. The series was written to promote the transfer of know-how concerning maintenance and repair of concrete structures. Use has been made of know-how developed in the

  9. VEHICLE REPAIR AND MAINTENANCE COSTS IN NIGERIA ...

    African Journals Online (AJOL)

    A standard model was established for the prediction of repair and maintenance costs of vehicles in a non-profit making government parastatal. The model was derived based on data collected over a ten year period from a non-profit making government parastatal, and it predicts repair and maintenance costs as a linear ...

  10. Remote maintenance development, July 1975--July 1976

    International Nuclear Information System (INIS)

    Fletcher, R.D.

    1977-04-01

    The results of the second year's efforts on remote handling development and studies for remote maintenance of failure-prone areas of the New Waste Calcining Facility (NWCF) are presented. Test arrangements and results for specific viewing situations and component remote installation and removal in the Remote Maintenance Development Facility (RMDF) and component material evaluations are discussed

  11. Putting Relationship Maintenance into Proper Perspectives.

    Science.gov (United States)

    Aleman, Carlos G.

    Noting that scholars often agree that varying investigations of relationship maintenance are only different mappings of the same territory, this paper organizes and criticizes the literature on relationship change from a position that defines maintenance as behavior that functions to sustain interpersonal bonds towards future interaction. The…

  12. Maintenance for the Millennium: Another Approach

    International Nuclear Information System (INIS)

    Sculthorpe, Barruy R.

    2002-01-01

    Nuclear units nationwide are struggling to increase plant reliability and availability while at the same time reduce their operating and maintenance costs. Some very costly investments have been made in programs such as Reliability Centered Maintenance [RCM]. Florida Power and Light's approach at the St. Lucie Nuclear Plant has taken a slightly different approach. Building on our knowledge of the RCM process and an already existing 'World Class' Predictive Maintenance Program, a 'Condition-Based' Maintenance Program that takes advantage of the RCM philosophy and our toolbox full of advanced and highly successful predictive maintenance technologies. These tools currently consist of vibration analysis, lubricant analysis (both physical property and wear metals analysis, thermographic analysis, motor current signature analysis, tribology and process parameter trending. All employed with the intent to evaluate a machines health. This machine health check allows the forecasting of future preventative maintenance [PM's] tasks and the revision of existing PM's to maximize machine performance and eliminate 'no-value-added' maintenance activities/costs. Within the last year, the Condition-Based Maintenance Program has produced a cost saving of approximately $1.5 million dollars. As the program matures, these cost savings will accumulate well into the millennium. (authors)

  13. Quantitative techniques for medical equipment maintenance management

    NARCIS (Netherlands)

    Ben Houria, Zeineb; Masmoudi, Malek; Al Hanbali, Ahmad; Khatrouch, Ikram; Masmoudi, Faouzi

    2016-01-01

    The maintenance department in a hospital is responsible for ensuring the safety of medical equipment and their availability while keeping the operation costs minimal. The selection of the best maintenance strategy is a key decision to reduce the equipment downtime, increase the availability, and

  14. A Dynamic Policy for Grouping Maintenance Activities

    NARCIS (Netherlands)

    R.E. Wildeman (Ralph); R. Dekker (Rommert); A.C.J.M. Smit

    1995-01-01

    textabstractA maintenance activity carried out on a technical system often involves a system-dependent set-up cost that is the same for all maintenance activities carried out on that system. Grouping activities thus saves costs since execution of a group of activities requires only one set-up. Many

  15. The effect of maintenance policy violations

    NARCIS (Netherlands)

    Rijsdijk, Christiaan; Tinga, Tiedo

    2014-01-01

    Motivation Maintenance policy assessments usually rely on expert judgement. We seek for some history based validation. Organisations may use our inference to assess risks of maintenance policy violations. Approach We depart from the arbitrary viewpoint that decisions have observable effects. We

  16. DEVELOPMENT OF A MAINTENANCE SCHEDULING MODEL FOR ...

    African Journals Online (AJOL)

    ... of minor maintenance, for each machine within this time span. In order to minimize the total cost of repairs and production. A numerical application of this development model in a case study is presented. Key words: Maintenance, modeling, scheduling, optimization. [Global Jnl Engineering Res. Vol.1(2) 2002: 107-118] ...

  17. Operation and maintenance of nuclear power plants

    International Nuclear Information System (INIS)

    Ackermann, G.

    1987-01-01

    This textbook gives a systematic introduction into the operational and maintenance activities in nuclear power plants with pressurized water reactors. Subjects: (1) Setup and operational behaviour of power reactors, (2) setup of nuclear power plants, (3) radiation protection and nuclear safety, (4) nuclear fuel, (5) constructional layout of nuclear power plants, (6) management, and (7) maintenance. 158 figs., 56 tabs

  18. Information Technology Applications to Maintenance Management

    Institute of Scientific and Technical Information of China (English)

    Abdul Raouf S.I.

    2003-01-01

    Application of IT (Information Technology) to equipment management have not been ade-quately made. A system using Benefit Evaluation Ladder is described for evaluating the need for IT ap-plications to maintenance management. A systematic evaluation scheme for Computerized Maintenance Management System (CMMS) is presented.

  19. Maintenance program guidelines for programmatic equipment

    International Nuclear Information System (INIS)

    1994-11-01

    The Division Directors at Lawrence Berkeley Laboratory are responsible for implementing a maintenance program for research equipment (also referred to as programmatic equipment) assigned to them. The program must allow maintenance to be accomplished in a manner that promotes operational safety, environmental protection and compliance, and cost effectiveness; that preserves the intended functions of the facilities and equipment; and that supports the programmatic mission of the Laboratory. Programmatic equipment -- such as accelerators, lasers, radiation detection equipment, and glove boxes -- is dedicated specifically to research. Installed equipment, by contrast, includes the mechanical and electrical systems installed as part of basic building construction, equipment essential to the normal functioning of the facility and its intended use. Examples of installed equipment are heating, ventilating, and air conditioning systems; elevators; and communications systems. The LBL Operating and Assurance Program Plan (PUB-3111, Revision 4) requires that a maintenance program be prepared for programmatic equipment and defines the basic maintenance program elements. Such a program of regular, documented maintenance is vital to the safety and quality of research activities. As a part of that support, this document offers guidance to Laboratory organizations for developing their maintenance programs. It clarifies the maintenance requirements of the Operating and Assurance Program (OAP) and presents an approach that, while not the only possibility, can be expected to produce an effective maintenance program for research equipment belonging to the Laboratory's organizations

  20. Reliability centered maintenance streamlining through lessons learned

    International Nuclear Information System (INIS)

    Strong, D.K.

    1991-01-01

    In late 1986, PSE and G concluded that the Nuclear Department would develop a consistent approach to maintenance at Artificial Island (Salem and Hope Creak nuclear units). Preventive maintenance (PM) would be the heart of this approach. In the last six months of 1987 departments affected by the maintenance program participated on working groups that developed the Artificial Island maintenance philosophy. The central theme of the maintenance philosophy is the RCM (reliability centered maintenance) process. A pilot project tested the process in 1988. In 1989 the Central PM Group formed and in 1990 was given responsibility and authority to analyze, approve, implement, and control PM program changes. RCM is the central theme of the PM improvement effort but not the whole effort. Other important pieces included in this paper are: development of a common PM program, improvement of work instructions, development of predictive maintenance techniques into programs, development of a PM basis database, development of PM feedback from failure trends, root cause analysis, maintenance performance indicators, technicians, and engineers

  1. Asbestos exposure of building maintenance personnel.

    Science.gov (United States)

    Mlynarek, S; Corn, M; Blake, C

    1996-06-01

    The exposures of building maintenance personnel and occupants to airborne asbestos fibers, and the effects of operations and maintenance programs on those exposures, continue to be an important public health issue. The subject of this investigation was a large metropolitan county with numerous public buildings which routinely conducted air sampling for asbestos. A total of 302 personal air samples in nine task categories collected during maintenance worker activities in proximity to asbestos-containing materials were analyzed; 102 environmental air samples in four task categories were also analyzed. The arithmetic means of the 8-hr time weighted average exposures for personal sampling for each task category were all below the Occupational Safety and Health Administration permissible exposure level of 0.1 fibers (f)/cc > 5 microm. The highest mean 8-hr time weighted average exposure was 0.030 f/cc > 5 microm for ceiling tile replacement. The maximum asbestos concentration during sample collection for environmental samples was 0.027 f/cc > 5 microm. All asbestos-related maintenance work was done within the framework of an Operations and Maintenance Program (OMP) which utilized both personal protective equipment and controls against fiber release/dispersion. Results are presented in association with specific OMP procedures or controls. These results support the effectiveness of using Operations and Maintenance Programs to manage asbestos in buildings without incurring unacceptable risk to maintenance workers performing maintenance tasks.

  2. Plumbing. Building Maintenance. Module III. Instructor's Guide.

    Science.gov (United States)

    Brunk, Art

    This curriculum guide, one of six modules keyed to the building maintenance competency profile developed by industry and education professionals, provides materials for a plumbing procedures unit of seven lessons. Lesson topics are: safety procedures, joining pipes, water systems, fixtures and appliances, maintenance and repair, drainage systems,…

  3. Deficiency within pavement Maintenance Organization in Tanzania ...

    African Journals Online (AJOL)

    In addition, the rules and regulations of the public administrative system do not allow for an effective and efficient management of road maintenance. Lack of equipment and adequate qualified personnel in the field of road maintenance is too an added deficiency. As a consequence, Tanzania has started to put road ...

  4. The mitochondrial uncoupling proteins

    OpenAIRE

    Ledesma, Amalia; de Lacoba, Mario García; Rial, Eduardo

    2002-01-01

    The uncoupling proteins (UCPs) are transporters, present in the mitochondrial inner membrane, that mediate a regulated discharge of the proton gradient that is generated by the respiratory chain. This energy-dissipatory mechanism can serve functions such as thermogenesis, maintenance of the redox balance, or reduction in the production of reactive oxygen species. Some UCP homologs may not act as true uncouplers, however, and their activity has yet to be defined. The UCPs are integral membrane...

  5. Maintenance Approaches for Different Production Methods

    Directory of Open Access Journals (Sweden)

    Mungani, Dzivhuluwani Simon

    2013-11-01

    Full Text Available Various production methods are used in industry to manufacture or produce a variety of products needed by industry and consumers. The nature of a product determines which production method is most suitable or cost-effective. A continuous process is typically used to produce large volumes of liquids or gases. Batch processing is often used for small volumes, such as pharmaceutical products. This paper discusses a research project to determine the relationship between maintenance approaches and production methods. A survey was done to determine to what extent three maintenance approaches reliability-centred maintenance (RCM, total productive maintenance (TPM, and business-centred maintenance (BCM are used for three different processing methods (continuous process, batch process, and a production line method.

  6. Sensor/signal monitoring and plant maintenance

    International Nuclear Information System (INIS)

    Ciftcioglu, Oe.; Tuerkcan, E.

    1994-02-01

    Nuclear Power Plant (NPO) availability is determined by the intended functionality of safety related system and components. Therefore, maintenance is an important issue in a power plant connected to the plant's reliability and safety. The traditional maintenance policies proved to be rather costly and even not effectively addressing NPP requirements. Referring to these drawbacks, in the last decade, in the nuclear reliability centered maintenance (RCM) gained substantial interest due to its merits. In the formal implementation of RCM, apparently, predictive maintenance is not considered. However, with the impact of modern real-time and on-line surveillance and monitoring methodologies, the predictive maintenance procedures like sensor/signal verification and validation are to be included into RCM. (orig.)

  7. Environmental favourable foundries through maintenance activities

    Directory of Open Access Journals (Sweden)

    A. Saniuk

    2015-10-01

    Full Text Available Maintenance today contributes to the aim of sustainable development in society, including environmental and energy saving, safety and economical aspects. The main purpose of the paper is to present the role of maintenance in the realization of sustainable developing practices in a foundry industry. Maintenance offers numerous opportunities to reduce the influence of foundry processes on the natural environment and utilize resources more efficiently. Maintenance activities can help to reduce losses and thereby improve the efficiency of the processes used in the production of resources (e.g. raw materials, energy, etc., especially in areas such as: the choice of an appropriate strategy for the maintenance of machinery and equipment (e.g. reactive, preventive, proactive; inventory management of spare parts; lubrication management and management of technology media.

  8. Maintenance considerations of the Starfire Commercial Tokamak

    International Nuclear Information System (INIS)

    Trachael, C.A.; Zahn, H.S.; Field, R.E.; Stevens, H.C.

    1979-01-01

    An initial reactor design has been developed for the Starfire Tokamak reactor that incorporates maintenance features to permit reactor and plant operation with a 75% availability. All components of the reactor can be replaced utilizing highly automated remote maintenance techniques. Provisions for contact maintenance are included but would only be utilized on a contingency basis since it is believed that allowable dose rates will be decreased in future years. A modular design approach is used for the reactor and auxiliary subsystems to permit efficient use of remote maintenance. The modular approach minimizes the number of different maintenance operations required and can result in use of simple tasks such as push, pull, turn, etc., maneuvers for module removal. Fault isolation is provided for each replaceable module

  9. Fatty acid composition of adipose tissue triglycerides after weight loss and weight maintenance

    DEFF Research Database (Denmark)

    Kunešová, M; Hlavatý, P; Tvrzická, E

    2012-01-01

    Fatty acid composition of adipose tissue changes with weight loss. Palmitoleic acid as a possible marker of endogenous lipogenesis or its functions as a lipokine are under debate. Objective was to assess the predictive role of adipose triglycerides fatty acids in weight maintenance in participants...... of the DIOGENES dietary intervention study. After an 8-week low calorie diet (LCD) subjects with > 8 % weight loss were randomized to 5 ad libitum weight maintenance diets for 6 months: low protein (P)/low glycemic index (GI) (LP/LGI), low P/high GI (LP/HGI), high P/low GI (HP/LGI), high P/high GI (HP....../HGI), and a control diet. Fatty acid composition in adipose tissue triglycerides was determined by gas chromatography in 195 subjects before the LCD (baseline), after LCD and weight maintenance. Weight change after the maintenance phase was positively correlated with baseline adipose palmitoleic (16:1n-7...

  10. Innovative techniques in maintenance training

    International Nuclear Information System (INIS)

    Soileau, J.B.; Blackwell, C.; Rackos, N.; Elmer, L.B.

    1991-01-01

    Performance based training has its beginning with a Job and Task Analysis (JTA), and culminates with the presentation of the developed training material. For optimum training effectiveness, a post training feedback mechanism is utilized to update and/or upgrade material content. A Job Task Analysis uses subject matter experts and supporting documentation to define the skills and knowledge necessary to perform functions from total position responsibilities to the simplest tasks, depending on desired results. Once skills and knowledge are defined, decisions on needed curriculum can be made including objectives, exams, and training settings (classroom, lab, or job site). This focused curriculum and the determination of best training settings are innovations of the performanced based training system. Past training experience has illustrated that a baseline level of classroom training acts as a catalyst for optimum hands-on training. The trainee exits the training phase able to do the job proficiently. This provides the training customer with quality training with minimum investment. Maintenance Training at Comanche Peak Steam Electric Station (CPSES) integrates the knowledge and skill needs by providing the individual with focused classroom presentations followed by sufficient laboratory time to practice actual task activities. Labs are configured to represent plant equipment and work environment to the maximum extent possible and duplicate in-plant work orders and procedures are used. This allows actions to be analyzed and corrective feedback given as necessary. As an added benefit, any mistakes made during training has minimal, if any, impact on plant performance

  11. Telomere Maintenance Mechanisms in Cancer

    Directory of Open Access Journals (Sweden)

    Tiago Bordeira Gaspar

    2018-05-01

    Full Text Available Tumour cells can adopt telomere maintenance mechanisms (TMMs to avoid telomere shortening, an inevitable process due to successive cell divisions. In most tumour cells, telomere length (TL is maintained by reactivation of telomerase, while a small part acquires immortality through the telomerase-independent alternative lengthening of telomeres (ALT mechanism. In the last years, a great amount of data was generated, and different TMMs were reported and explained in detail, benefiting from genome-scale studies of major importance. In this review, we address seven different TMMs in tumour cells: mutations of the TERT promoter (TERTp, amplification of the genes TERT and TERC, polymorphic variants of the TERT gene and of its promoter, rearrangements of the TERT gene, epigenetic changes, ALT, and non-defined TMM (NDTMM. We gathered information from over fifty thousand patients reported in 288 papers in the last years. This wide data collection enabled us to portray, by organ/system and histotypes, the prevalence of TERTp mutations, TERT and TERC amplifications, and ALT in human tumours. Based on this information, we discuss the putative future clinical impact of the aforementioned mechanisms on the malignant transformation process in different setups, and provide insights for screening, prognosis, and patient management stratification.

  12. Spares management for improved maintenance

    International Nuclear Information System (INIS)

    Moncrief, E.C.; Schroder, R.M.

    1987-01-01

    A recent survey by the US Nuclear Regulatory Commission (NRC) of maintenance practices at US nuclear power plants confirms that spare parts availability is, to some extent, a problem at all but a few plants, and that 57% of the plants sometimes operated in a limiting condition for operation over the last year due to unavailability of spare parts. That's the bad news. The good news is that significant improvement is possible without ballooning inventory. The vast majority of total dollar value of spare parts inventory at power generation stations, whether nuclear or fossil, is rarely used (parts or components that are used 12 or less times per year including many with a history of no use for many years). Inventory Solutions of Akron, Ohio, has developed such a tool, called rarely used inventory stocking logic (RUSL) which is a user-friendly decision support tool for setting spare parts reorder points. Based on statistical computations enhanced to include the most advanced techniques developed for use in aerospace and military combat readiness, RUSL calculates the optimum stocking level to achieve the desired spare part availability at the station. In doing so, it considers the importance of the part in preventing an outage, as well as key economic ordering parameters, such as part cost, carrying cost, number of parts in service, historical observed usage, anticipated future usage, and lead time to replenish

  13. Value maximizing maintenance policies under general repair

    International Nuclear Information System (INIS)

    Marais, Karen B.

    2013-01-01

    One class of maintenance optimization problems considers the notion of general repair maintenance policies where systems are repaired or replaced on failure. In each case the optimality is based on minimizing the total maintenance cost of the system. These cost-centric optimizations ignore the value dimension of maintenance and can lead to maintenance strategies that do not maximize system value. This paper applies these ideas to the general repair optimization problem using a semi-Markov decision process, discounted cash flow techniques, and dynamic programming to identify the value-optimal actions for any given time and system condition. The impact of several parameters on maintenance strategy, such as operating cost and revenue, system failure characteristics, repair and replacement costs, and the planning time horizon, is explored. This approach provides a quantitative basis on which to base maintenance strategy decisions that contribute to system value. These decisions are different from those suggested by traditional cost-based approaches. The results show (1) how the optimal action for a given time and condition changes as replacement and repair costs change, and identifies the point at which these costs become too high for profitable system operation; (2) that for shorter planning horizons it is better to repair, since there is no time to reap the benefits of increased operating profit and reliability; (3) how the value-optimal maintenance policy is affected by the system's failure characteristics, and hence whether it is worthwhile to invest in higher reliability; and (4) the impact of the repair level on the optimal maintenance policy. -- Highlights: •Provides a quantitative basis for maintenance strategy decisions that contribute to system value. •Shows how the optimal action for a given condition changes as replacement and repair costs change. •Shows how the optimal policy is affected by the system's failure characteristics. •Shows when it is

  14. Optimum policies for a system with general imperfect maintenance

    International Nuclear Information System (INIS)

    Sheu, S.-H.; Lin, Y.-B.; Liao, G.-L.

    2006-01-01

    This study considers periodic preventive maintenance policies, which maximizes the availability of a repairable system with major repair at failure. Three types of preventive maintenance are performed, namely: imperfect preventive maintenance (IPM), perfect preventive maintenance (PPM) and failed preventive maintenance (FPM). The probability that preventive maintenance is perfect depends on the number of imperfect maintenances conducted since the previous renewal cycle, and the probability that preventive maintenance remains imperfect is not increasing. The optimum preventive maintenance time that maximizes availability is derived. Various special cases are considered. A numerical example is given

  15. Maintenance and Outage Management Assessment (MOMA)

    International Nuclear Information System (INIS)

    2005-01-01

    The competitive environment has significant implications for nuclear power plant (NPP) operations, which include, inter alia, the need for efficient use of resources and effective management of plant activities of maintenance and outages. The purpose of NPP maintenance and outages is to allow NPPs to use all those functions necessary for safe and reliable power production by keeping them available and adequate maintenance programme is essential. The maintenance programme covers all preventive and remedial measures, both administrative and technical, necessary to identify and mitigate degradation of a functioning system, structure or component, or restore the design functions of a failed system, structure or component to an acceptable level. In response to the needs of MSs, NPES (Nuclear Power Section, Division of Nuclear Power, IAEA) plans to strengthen its services. NPES services will not only continue to provide its 'traditional' products of publications of nuclear industrial best practices and technical implementation of TC projects on plant maintenance and outage management, but also be expanded to deliver, in a timely manner, technical support missions as requested by MSs for NPPs. One of many services is Maintenance and Outage Management Assessment (MOMA). The NPP can obtain support and assistance in assessment and optimisation of its maintenance program and/or outage management. It aims to help the NPP improve its performance of maintenance and outage in a competitive nuclear power business environment. The specific benefits of the assessment are as follows: a) disseminate nuclear industrial best practices on maintenance program and outage management in the world, b) benchmark, evaluate and optimise the approach of maintenance program and outage management and c) identify solutions to known problems at nuclear power plants, if any. MOMA is conducted at the request of NPPs of any IAEA Member States. MOMA consists in a technical mission/visit for 1-3 weeks by

  16. Systematic maintenance analysis with decision support method and tool for optimizing maintenance programme

    International Nuclear Information System (INIS)

    Laakso, K.; Simola, K.; Dorrepaal, J.; Skogberg, P.

    1999-01-01

    This report describes an approach to evaluate the effectiveness of test and maintenance programs of technical systems used during several years. The method combines an analysis of the historical data on faults and repairs with an analysis of the history of periodic testing and preventive maintenance action programs. The application of the maintenance analysis from the methodological point of view in the reliability centered maintenance (RCM) project for Barsebaeck nuclear power plant is described. In order to limit the analysis resources, a method for ranking of objects for maintenance analysis is needed. Preliminary suggestions for changes in maintenance action programs are based on signals from simple maintenance indicators and qualitative analysis of underlying data on failures and maintenance. To facilitate generation of maintenance indicators, and make the maintenance analysis more efficient, a powerful and suitable data treatment tool is needed for analysis of the work order history. In the final maintenance decisions, additional decision criteria must be taken into account, and thus a more formal decision analysis is often needed for decision support. (au)

  17. Proceedings of the fourth international conference on CANDU maintenance

    International Nuclear Information System (INIS)

    1997-01-01

    These proceedings record the information presented at the 4th International Conference on CANDU Maintenance held November 16-18,1997 in Toronto, Canada. The papers for these proceedings were prepared on component maintenance, human performance, steam generator leak detection, fuel channel inspections, rotating equipment maintenance, surveillance programs, inspection techniques, valve maintenance, steam generator repairs and performance, reactor aging management and preventative maintenance

  18. Proceedings of the fourth international conference on CANDU maintenance

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-07-01

    These proceedings record the information presented at the 4th International Conference on CANDU Maintenance held November 16-18,1997 in Toronto, Canada. The papers for these proceedings were prepared on component maintenance, human performance, steam generator leak detection, fuel channel inspections, rotating equipment maintenance, surveillance programs, inspection techniques, valve maintenance, steam generator repairs and performance, reactor aging management and preventative maintenance.

  19. Operation and maintenance of the technical installations in buildings

    DEFF Research Database (Denmark)

    Nielsen, O.(red.)

    The report contains twelve papers from a seminar on operation and maintenance, held at the Danish Building Research Institute in October 1976. The papers deal, among other things, with dimensioning and balancing of pipesystems, design of ventilating systems for adequate operation and maintenance,......, cost and quality in maintenance, maintenance service companies, as well as organization and training for building services maintenance....

  20. Magnetically Attached Multifunction Maintenance Rover

    Science.gov (United States)

    Bar-Cohen, Yoseph; Joffe, Benjamin

    2005-01-01

    A versatile mobile telerobot, denoted the magnetically attached multifunction maintenance rover (MAGMER), has been proposed for use in the inspection and maintenance of the surfaces of ships, tanks containing petrochemicals, and other large ferromagnetic structures. As its name suggests, this robot would utilize magnetic attraction to adhere to a structure. As it moved along the surface of the structure, the MAGMER would perform tasks that could include close-up visual inspection by use of video cameras, various sensors, and/or removal of paint by water-jet blasting, laser heating, or induction heating. The water-jet nozzles would be mounted coaxially within compressed-air-powered venturi nozzles that would collect the paint debris dislodged by the jets. The MAGMER would be deployed, powered, and controlled from a truck, to which it would be connected by hoses for water, compressed air, and collection of debris and by cables for electric power and communication (see Figure 1). The operation of the MAGMER on a typical large structure would necessitate the use of long cables and hoses, which can be heavy. To reduce the load of the hoses and cables on the MAGMER and thereby ensure its ability to adhere to vertical and overhanging surfaces, the hoses and cables would be paid out through telescopic booms that would be parts of a MAGMER support system. The MAGMER would move by use of four motorized, steerable wheels, each of which would be mounted in an assembly that would include permanent magnets and four pole pieces (see Figure 2). The wheels would protrude from between the pole pieces by only about 3 mm, so that the gap between the pole pieces and the ferromagnetic surface would be just large enough to permit motion along the surface but not so large as to reduce the magnetic attraction excessively. In addition to the wheel assemblies, the MAGMER would include magnetic adherence enhancement fixtures, which would comprise arrays of permanent magnets and pole pieces

  1. Effective maintenance practices to manage system aging

    International Nuclear Information System (INIS)

    Chockie, A.; Bjorkelo, K.

    1992-01-01

    For a variety of economic and technical reasons, there has been a growing concern with the aging of complex systems and components and the role that maintenance can play in reducing this degradation. A study for the Nuclear Regulatory Commission was recently undertaken to identify effective maintenance practices that could be adapted by the nuclear industry in the United States to assist in managing the aging degradation of plant systems and components. Four organizations were examined to assess the influence that their maintenance programs have on their ability to address the systems and component aging degradation issues. An effective maintenance program was found to be essential to the management of system and component aging. The four key elements of an effective maintenance program that are important to an aging management program were identified. These are: the selection of critical systems and components; the development of an understanding of aging through the collection and analysis of equipment performance information; the development of appropriate preventive and predictive maintenance tasks to manage equipment and system aging degradation; the use of feedback mechanisms to continuously improve the management of aging systems and components. These elements were found to be common to all four organizations. In examining how the four organizations have structured their maintenance programs to include these key elements provides valuable lessons not only for the nuclear power industry, but also for any industrial organization that is concerned with the management of system and component aging degradation. This document provides detail, of these studies

  2. Implementation of maintenance rule for structures

    International Nuclear Information System (INIS)

    Ashar, H.; Bagchi, G.

    1999-01-01

    The maintenance rule, 10 CFR 50.65, 'Requirements for Monitoring the Effectiveness of Maintenance at Nuclear Power Plants', was published by the Nuclear Regulatory Commission (NRC) in the Federal Register (56 FR 31324) on July 10, 1991. The rule became effective on July 10, 1996, giving nuclear power plant licensees 5 years to implement it. During 1994-1995, NRC staff visited nine nuclear power plant sites to observe licensees' preparations for implementation of the rule. The teams found that most of the licensees had not established goals, or performance criteria for monitoring structures at their sites. The licensees contended that the structures were inherently reliable and required no monitoring under the maintenance rule. On the basis of earlier site visits performed by NRC staff to assess the condition of structures, the NRC staff could not accept this contention, and clarified its position in Revision 1 of Regulatory Guide 1.160, 'Monitoring the Effectiveness of Maintenance at Nuclear Power Plants'. This paper discusses the applicability of the maintenance rule criteria for structures and its usefulness in ensuring that the structures, systems, and components within the scope of the maintenance rule are capable of fulfilling their intended functions. Also discussed are the aspects of maintenance rule efforts that could be useful for license renewal applications. (orig.)

  3. A VITAL service for predictive MOV maintenance

    International Nuclear Information System (INIS)

    Anon.

    1990-01-01

    Motor-operated valve (MOV) maintenance can be a major contributor to unplanned plant downtime, either because valve malfunctions cause unscheduled outages, or because unnecessary valve maintenance lengthens scheduled outages. The cause of both situations is a preventive approach to valve maintenance, where maintenance is scheduled according to a timetable instead of according to a demonstrated need. To move towards a predictive maintenance approach, inexpensive methods of collecting and diagnosing real-time data on the conditions of a valve must be made available. Such a system must be capable of detecting and diagnosing degrading mechanical and electrical behaviour at an early stage, as well as being able to predict the time of failure. Without this data, a predictive approach to maintenance is impossible. Westinghouse is addressing the requirements for a predictive approach to MOV maintenance with the introduction of a third-generation of diagnostic systems for motor-operated valves -the portable Valve Intelligent Test and Analysis System (VITALS) and the on-line Valve Monitoring System (VMS). (author)

  4. Maintenance Energy Requirements of Double-Muscled Belgian Blue Beef Cows

    Science.gov (United States)

    Fiems, Leo O.; De Boever, Johan L.; Vanacker, José M.; De Campeneere, Sam

    2015-01-01

    Simple Summary Double-muscled Belgian Blue animals are extremely lean, characterized by a deviant muscle fiber type with more fast-glycolytic fibers, compared to non-double-muscled animals. This fiber type may result in lower maintenance energy requirements. On the other hand, lean meat animals mostly have a higher rate of protein turnover, which requires more energy for maintenance. Therefore, maintenance requirements of Belgian Blue cows were investigated based on a zero body weight gain. This technique showed that maintenance energy requirements of double-muscled Belgian Blue beef cows were close to the mean requirements of cows of other beef genotypes. Abstract Sixty non-pregnant, non-lactating double-muscled Belgian Blue (DMBB) cows were used to estimate the energy required to maintain body weight (BW). They were fed one of three energy levels for 112 or 140 days, corresponding to approximately 100%, 80% or 70% of their total energy requirements. The relationship between daily energy intake and BW and daily BW change was developed using regression analysis. Maintenance energy requirements were estimated from the regression equation by setting BW gain to zero. Metabolizable and net energy for maintenance amounted to 0.569 ± 0.001 and 0.332 ± 0.001 MJ per kg BW0.75/d, respectively. Maintenance energy requirements were not dependent on energy level (p > 0.10). Parity affected maintenance energy requirements (p < 0.001), although the small numerical differences between parities may hardly be nutritionally relevant. Maintenance energy requirements of DMBB beef cows were close to the mean energy requirements of other beef genotypes reported in the literature. PMID:26479139

  5. Improving regulatory oversight of maintenance programs

    International Nuclear Information System (INIS)

    Cook, S.

    2008-01-01

    Safe nuclear power plant operation requires that risks due to failure or unavailability of Structures, Systems and Components (SSCs) be minimized. Implementation of an effective maintenance program is a key means for achieving this goal. In its regulatory framework, the important relationship between maintenance and safety is acknowledged by the CNSC. A high level maintenance program requirement is included in the Class I Facilities Regulations. In addition, the operating licence contains a condition based on the principle that the design function and performance of SSCs needs to remain consistent with the plant's design and analysis documents. Nuclear power plant licensees have the primary responsibility for safe operation of their facilities and consequently for implementation of a successful maintenance program. The oversight role of the Canadian Nuclear Safety Commission (CNSC) is to ensure that the licensee carries out that responsibility. The challenge for the CNSC is how to do this consistently and efficiently. Three opportunities for improvement to regulatory maintenance oversight are being pursued. These are related to the regulatory framework, compliance verification inspection activities and monitoring of self-reporting. The regulatory framework has been improved by clarifying expectations through the issuance of S-210 'Maintenance Programs for Nuclear Power Plants'. Inspection activities have been improved by introducing new maintenance inspections into the baseline program. Monitoring is being improved by making better use of self-reported and industry produced maintenance related performance indicators. As with any type of program change, the challenge is to ensure the consistent and optimal application of regulatory activities and resources. This paper is a summary of the CNSC's approach to improving its maintenance oversight strategy. (author)

  6. Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

    DEFF Research Database (Denmark)

    Pinela da Silva, Sonia Cristina; Altmannova, Veronika; Luke-Glaser, Sarah

    2016-01-01

    Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro...

  7. Maintenance Optimization of High Voltage Substation Model

    Directory of Open Access Journals (Sweden)

    Radim Bris

    2008-01-01

    Full Text Available The real system from practice is selected for optimization purpose in this paper. We describe the real scheme of a high voltage (HV substation in different work states. Model scheme of the HV substation 22 kV is demonstrated within the paper. The scheme serves as input model scheme for the maintenance optimization. The input reliability and cost parameters of all components are given: the preventive and corrective maintenance costs, the actual maintenance period (being optimized, the failure rate and mean time to repair - MTTR.

  8. Long Length Contaminated Equipment Maintenance Plan

    International Nuclear Information System (INIS)

    ESVELT, C.A.

    2000-01-01

    The purpose of this document is to provide the maintenance requirements of the Long Length Contaminated Equipment (LLCE) trailers and provide a basis for the maintenance frequencies selected. This document is applicable to the LLCE Receiver trailer and Transport trailer assembled by Mobilized Systems Inc. (MSI). Equipment used in conjunction with, or in support of, these trailers is not included. This document does not provide the maintenance requirements for checkout and startup of the equipment following the extended lay-up status which began in the mid 1990s. These requirements will be specified in other documentation

  9. EBR-II rotating plug seal maintenance

    International Nuclear Information System (INIS)

    Allen, K.J.

    1986-01-01

    The EBR-II rotating plug seals require frequent cleaning and maintenance to keep the plugs from sticking during fuel handling. Time consuming cleaning on the cover gas and air sides of the dip ring seal is required to remove oxidation and sodium reaction products that accumulate and stop plug rotation. Despite severely limited access, effective seal cleaning techniques have removed 11 800 lb (5 352 kg) of deposits from the seals since 1964. Temperature control modifications and repairs have also required major maintenance work. Suggested seal design recommendations could significantly reduce maintenance on future similar seals

  10. Radwaste treatment complex. DRAWMACS planned maintenance system

    International Nuclear Information System (INIS)

    Keel, A.J.

    1992-07-01

    This document describes the operation of the Planned Maintenance System for the Radwaste Treatment Complex. The Planned Maintenance System forms part of the Decommissioning and Radwaste Management Computer System (DRAWMACS). Further detailed information about the data structure of the system is contained in Database Design for the DRAWMACS Planned Maintenance System (AEA-D and R-0285, 2nd issue, 25th February 1992). Information for other components of DRAWMACS is contained in Basic User Guide for the Radwaste Treatment Plant Computer System (AEA-D and R-0019, July 1990). (author)

  11. Performance based regulation - The maintenance rule

    Energy Technology Data Exchange (ETDEWEB)

    Correia, Richard P. [NRR/DOTS/TQMP, U.S. Nuclear Regulatory Commission, Office of Nuclear Reactor Regulation, M/S OWFN 10A19, Washington, D.C. 20555 (United States)

    1997-07-01

    The U.S. Nuclear Regulatory Commission has begun a transition from 'process-oriented' to 'results-oriented' regulations. The maintenance rule is a results-oriented rule that mandates consideration of risk and plant performance. The Maintenance Rule allows licensees to devise the most effective and efficient means of achieving the results described in the rule including the use of Probabilistic Risk (or Safety) Assessments. The NRC staff conducted a series of site visits to evaluate implementation of the Rule. Conclusions from the site visits indicated that the results-oriented Maintenance Rule can be successfully implemented and enforced. (author)

  12. Operation and maintenance of offshore wind farms

    DEFF Research Database (Denmark)

    Petersen, Kristian R.; Madsen, Erik Skov; Bilberg, Arne

    The offshore wind industry is booming and larger, more efficient wind-turbines have constantly been introduced into the market. However, research within the field of the operation and maintenance (O&M) of offshore wind farms is limited as the field is still immature. In this paper, two current ma...... maintenance models - RCM and TPM - are discussed in this context. Furthermore, through a case study, the paper looks into Modularization and SSLP, as these concepts can be utilized to optimize maintenance. This is a new approach for the offshore wind industry....

  13. Maintenance of the CERN telephone exchanges

    CERN Multimedia

    2007-01-01

    Maintenance work will be carried out on the CERN telephone exchanges between 8 p.m. and midnight on 20 November. Fixed-line telephone and audioconference services may be disrupted while the work is being carried out. However, the CCC and the fire brigade will be contactable at any time. Mobile telephony services (GSM) will not be affected by the maintenance work. For further details about the maintenance work, please contact the telephone switchboard on 76111 or by email to standard.telephone@cern.ch. Telecom Section IT/CS

  14. Maintenance of the CERN telephone exchanges

    CERN Multimedia

    2007-01-01

    Maintenance work will be carried out on the CERN telephone exchanges between 8 p.m. and midnight on 20 November. Fixed-line telephone and audioconference services may be disrupted while the work is being carried out. However, the CCC and the fire brigade will be reachable at any time. Mobile telephony services (GSM) will not be affected by the maintenance work. For further details about the maintenance work, please contact the telephone switchboard on 76111 or by email. Telecom Section - IT/CS

  15. Performance based regulation - The maintenance rule

    International Nuclear Information System (INIS)

    Correia, Richard P.

    1997-01-01

    The U.S. Nuclear Regulatory Commission has begun a transition from 'process-oriented' to 'results-oriented' regulations. The maintenance rule is a results-oriented rule that mandates consideration of risk and plant performance. The Maintenance Rule allows licensees to devise the most effective and efficient means of achieving the results described in the rule including the use of Probabilistic Risk (or Safety) Assessments. The NRC staff conducted a series of site visits to evaluate implementation of the Rule. Conclusions from the site visits indicated that the results-oriented Maintenance Rule can be successfully implemented and enforced. (author)

  16. Actin and myosin contribute to mammalian mitochondrial DNA maintenance

    Science.gov (United States)

    Reyes, A.; He, J.; Mao, C. C.; Bailey, L. J.; Di Re, M.; Sembongi, H.; Kazak, L.; Dzionek, K.; Holmes, J. B.; Cluett, T. J.; Harbour, M. E.; Fearnley, I. M.; Crouch, R. J.; Conti, M. A.; Adelstein, R. S.; Walker, J. E.; Holt, I. J.

    2011-01-01

    Mitochondrial DNA maintenance and segregation are dependent on the actin cytoskeleton in budding yeast. We found two cytoskeletal proteins among six proteins tightly associated with rat liver mitochondrial DNA: non-muscle myosin heavy chain IIA and β-actin. In human cells, transient gene silencing of MYH9 (encoding non-muscle myosin heavy chain IIA), or the closely related MYH10 gene (encoding non-muscle myosin heavy chain IIB), altered the topology and increased the copy number of mitochondrial DNA; and the latter effect was enhanced when both genes were targeted simultaneously. In contrast, genetic ablation of non-muscle myosin IIB was associated with a 60% decrease in mitochondrial DNA copy number in mouse embryonic fibroblasts, compared to control cells. Gene silencing of β-actin also affected mitochondrial DNA copy number and organization. Protease-protection experiments and iodixanol gradient analysis suggest some β-actin and non-muscle myosin heavy chain IIA reside within human mitochondria and confirm that they are associated with mitochondrial DNA. Collectively, these results strongly implicate the actomyosin cytoskeleton in mammalian mitochondrial DNA maintenance. PMID:21398640

  17. A Planned Preventive Maintenance Program. A Handbook for Chief Business Officers and Supervisors of Maintenance with Suggestions on Maintenance for Consideration by Presidents of Higher Institutions.

    Science.gov (United States)

    Daniel, Clarence H.

    This handbook explains planned preventive maintenance program, which is an operational system of maintenance designed to increase the effectiveness of the maintenance staff and the use of maintenance funds through efficient scheduling of inspections and follow-through of work to be performed. Sections are included for the chief administrative…

  18. Site Maintenance Plan: Part 2, Site Maintenance Action Plan for FY 1994

    Energy Technology Data Exchange (ETDEWEB)

    Fisk, E.L.

    1994-06-01

    This Fiscal Year (FY) 1994 Site Maintenance Action Plan (SMAP) is Part II of the Site Maintenance Plan, and has been written by Westinghouse Hanford Company (WHC) to outline the requirements stated in DOE Order 4330.4B, Maintenance Management Program, Chapter 1, Paragraph 3.3.1. The SMAP provides an annual status of maintenance initiatives completed and planned, a summary of performance indicators, a summary of maintenance backlog, a listing of real property and capital equipment maintenance cost estimates that were used to create the FY 1996 infrastructure and maintenance budget input, and a listing of proposed line item and general plant projects. Additionally, assumptions for various Site programs are listed to bring the Site Maintenance Plan into focus with overall Site activities. The primary mission at Hanford is to clean up the Site. In this cleanup process WHC will provide scientific and technological expertise to meet global needs, and partnership with stakeholders in the region to develop regional economic diversification. Other missions at the Hanford Site include energy research and development, and waste management and disposal activities. Their primary mission has a 30-year projected life span and will direct the shutting down and cleanup of defense production facilities and the Fast Flux Test Facility. This long-term mission requires continuous maintenance and in many instances, replacement of existing basic infrastructure, support facilities, and utilities. Without adequate maintenance and capital funding these infrastructure, support facilities, and utilities will continue to deteriorate causing an increase in backlogged work.

  19. Site Maintenance Plan: Part 2, Site Maintenance Action Plan for FY 1994

    International Nuclear Information System (INIS)

    Fisk, E.L.

    1994-06-01

    This Fiscal Year (FY) 1994 Site Maintenance Action Plan (SMAP) is Part II of the Site Maintenance Plan, and has been written by Westinghouse Hanford Company (WHC) to outline the requirements stated in DOE Order 4330.4B, Maintenance Management Program, Chapter 1, Paragraph 3.3.1. The SMAP provides an annual status of maintenance initiatives completed and planned, a summary of performance indicators, a summary of maintenance backlog, a listing of real property and capital equipment maintenance cost estimates that were used to create the FY 1996 infrastructure and maintenance budget input, and a listing of proposed line item and general plant projects. Additionally, assumptions for various Site programs are listed to bring the Site Maintenance Plan into focus with overall Site activities. The primary mission at Hanford is to clean up the Site. In this cleanup process WHC will provide scientific and technological expertise to meet global needs, and partnership with stakeholders in the region to develop regional economic diversification. Other missions at the Hanford Site include energy research and development, and waste management and disposal activities. Their primary mission has a 30-year projected life span and will direct the shutting down and cleanup of defense production facilities and the Fast Flux Test Facility. This long-term mission requires continuous maintenance and in many instances, replacement of existing basic infrastructure, support facilities, and utilities. Without adequate maintenance and capital funding these infrastructure, support facilities, and utilities will continue to deteriorate causing an increase in backlogged work

  20. Assessment of building maintenance in Nigerian university system ...

    African Journals Online (AJOL)

    Building maintenance involves preserving a building so that it can retain its economic value ... maintenance policy by employing outsourcing maintenance services. ... services including the use of information and communication technology.

  1. Effects of maintenance electroconvulsive therapy on cognitive functions

    NARCIS (Netherlands)

    Vothknecht, S.; Kho, K. H.; van Schaick, H. W.; Zwinderman, A. H.; Middelkoop, H.; Blansjaar, B. A.

    2003-01-01

    Background: This study examined cognitive side effects of maintenance electroconvulsive (ECT) in comparison with maintenance pharmacotherapy after index ECT. Method: Clinical outcome data and neuropsychological measurements were compared in 11 maintenance ECT patients and 13 control patients treated

  2. Application of preventive maintenance planning in a parquet ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-04-20

    Apr 20, 2009 ... Key words: Maintenance, preventive maintenance, enterprise, planning. INTRODUCTION .... There is machine introduction form on each machine. Moreover, ..... loy using total productive maintenance (TPM) approach. M.Sc.

  3. Plant Maintenance. The Licensee's Viewpoint

    International Nuclear Information System (INIS)

    Ungi, T.

    1997-01-01

    Plant maintenance is a very complex process which requires considerable effort from both within the maintenance process and also many support activities. It is important that the plant maintenance policy is translated into a maintenance programme which will define all relevant aspects of the maintenance. An aspect of this maintenance programme will be a maintenance catalogue which will define the maintenance activities to be carried out and at what frequencies. This paper is aimed at discussing the maintenance philosophy and resulting maintenance catalogues currently adopted in Nuclear Electric Ltd, and in particular at Heysham 2 Power Station. It goes on to consider whether these maintenance catalogues contain the optimum maintenance and if not should they be changed. If change is required, the process by which this change will be brought about is also discussed. (author)

  4. The Many Features of Stanford's Housing Maintenance Software.

    Science.gov (United States)

    Milshtein, Amy

    1999-01-01

    Explains how Stanford University custom designed its own building maintenance and administration software package: the Housing Operations Maintenance Enterprise Resource (HOMER). Describes how HOMER relieved facility maintenance staff from some archaic systems, and its development and functionality. (GR)

  5. Modularity Design Approach for Preventive Machine Maintenance

    Science.gov (United States)

    Ernawati, D.; Pudji, E.; Ngatilah, Y.; Handoyo, R.

    2018-01-01

    In a company, machine maintenance system will be very influential in production process activity. The company should have a scheduled engine maintenance system that does not require high costs when repairing and replacing machine parts. Modularity Design method is able to provide solutions to the engine maintenance scheduling system and can prevent fatal damage to the engine components. It can minimize the cost of repair and replacement of these machine components.The paper provides a solution to machine maintenance problems. The paper is also completed with case study of milling machines. That case studies can give us a real description about impact implementation of modularity design to prevent fatal damage to components and minimize the cost of repair and replacement of components of the machine.

  6. 24 CFR 266.507 - Maintenance requirements.

    Science.gov (United States)

    2010-04-01

    ... HOUSING FINANCE AGENCY RISK-SHARING PROGRAM FOR INSURED AFFORDABLE MULTIFAMILY PROJECT LOANS Project Management and Servicing § 266.507 Maintenance requirements. The mortgagor must maintain the project in...

  7. Performance measures for world class maintenance

    International Nuclear Information System (INIS)

    Labib, A.W.

    1998-01-01

    A main problem in maintenance in general, and in power plants and related equipment in particular, is the lack of a practical, consistent, and adaptive performance measure that provides a focused feedback and integrates preventive and corrective modes of maintenance. The presentation defines concepts of world class and benchmarking. Desirable features in an appropriate performance measure are identified. It then, demonstrates current practices in maintenance and criticises their shortcomings. An alternative model is presented through a case study. The model monitors performance from a general view, and then offers a focused analysis. The main conclusion is that the proposed model offers an adaptive and a dynamic framework, and hence production and maintenance are integrated in a 'real time' environment. The system is also flexible in working with any other criteria whether they are of a quantitative or a qualitative nature. (orig.) 16 refs

  8. Predictive Maintenance and Robotic System Design

    African Journals Online (AJOL)

    pc

    2018-03-05

    Mar 5, 2018 ... Business objectives include: reduce equipment maintenance time, reduce ... Data Acquisition: Proprietary machine diagnostic systems and sensors .... and Logistics, Volume #IPart# http://www.ifac-papersonline.net,. Identifier ...

  9. Early implementation of the Maintenance Rule

    International Nuclear Information System (INIS)

    Green, J.R.

    1995-01-01

    On July 10, 1991, the U.S. Nuclear Regulatory Commission (NRC) published the Maintenance Rule as Section 50.65 of 10 CFR 50. The purpose of the Maintenance Rule is to ensure that the effectiveness of maintenance activities is assessed on an ongoing basis in a manner that ensures key structures, systems, and components are capable of performing their intended function. Full implementation of the rule is required by July 10, 1996. On May 31, 1994, the NRC published Generic Letter 94-01, which allowed removal of technical specification accelerated testing and special reporting requirements for emergency diesel generators provided a program is implemented for monitoring and maintaining emergency diesel generator performance consistent with the provisions of the Maintenance Rule

  10. Modernizing international maintenance obligations in Serbia

    Directory of Open Access Journals (Sweden)

    Bordaš Bernadet

    2013-01-01

    Full Text Available The paper deals with the modernization of private international law of maintenance in Serbia in the light of the two newest international instruments drawn up by the Hague Conference on Private International Law. As Serbia has ratified the Hague Protocol on the Law Applicable to Maintenance Obligations in January 2013 introducing modern rules to the legal system of Serbia, the issue of ratification of the Hague Convention on the International Recovery of Child Support and Other Forms of Family Maintenance has arisen, in order to modernize this aspect of the international maintenance as well. In order to establish a position on the issue, the paper presents and analyzes the essential provisions of the Convention (scope of application, administrative cooperation, recognition and enforcement of decisions, stricto sensu enforcement. Based on the analyses, the concluding remarks offers some elements on the bases of which the competent authorities could form an opinion on the necessity and desirability of the ratification of the Convention.

  11. A human factors approach to effective maintenance

    International Nuclear Information System (INIS)

    Penington, J.; Shakeri, S.

    2006-01-01

    Traditionally in the field of Human Factors within the nuclear industry, the focus has been to identify the potential for human errors in operating tasks, and develop strategies to prevent their occurrence, provide recovery mechanisms, and mitigate the consequences of error as appropriate. Past experience has demonstrated however a significant number of human errors within the nuclear industry occur during maintenance tasks. It is for this reason, and the fact that our nuclear power plants are ageing and increasingly in need of maintenance, that the industry must pay more attention to maintenance tasks. The purpose of this paper is to present a framework for effective maintenance programs, and based upon this framework discuss an approach (an audit tool) that can be used to both design such a program, and to assess existing programs. In addition, this tool can form the basis of cost benefit decisions relating to priorities for improvements to existing programs. (author)

  12. Progress with the national infrastructure maintenance strategy

    CSIR Research Space (South Africa)

    Wall, K

    2008-07-01

    Full Text Available infrastructure investment and maintenance that will result from this strategy will not only improve infrastructure performance and underpin services sustainability, but will also contribute significantly towards national and local economic growth and will add...

  13. Industrial relations: workings of the maintenance strike.

    Science.gov (United States)

    Davidson, N

    1978-11-10

    The frustrations leading to the maintenance dispute go back four years. Author highlights the NHS's inability to handle internal disagreements, and the problems of a bureaucratic and politically-directed profession.

  14. Gravel Roads: Maintenance and Design Manual

    Science.gov (United States)

    This manual was developed with a major emphasis on the maintenance of gravel roads, including some basic design elements. The purpose of the manual is to provide clear and helpful information for doing a better job of maintaining gravel roads.

  15. Advanced decision support for winter road maintenance

    Science.gov (United States)

    2008-01-01

    This document provides an overview of the Federal Highway Administration's winter Maintenance Decision Support System (MDSS). The MDSS is a decision support tool that has the ability to provide weather predictions focused toward the road surface. The...

  16. Analysis and modeling of rail maintenance costs

    Directory of Open Access Journals (Sweden)

    Amir Ali Bakhshi

    2012-01-01

    Full Text Available Railroad maintenance engineering plays an important role on availability of roads and reducing the cost of railroad incidents. Rail is of the most important parts of railroad industry, which needs regular maintenance since it covers a significant part of total maintenance cost. Any attempt on optimizing total cost of maintenance could substantially reduce the cost of railroad system and it can reduce total cost of the industry. The paper presents a new method to estimate the cost of rail failure using different cost components such as cost of inspection and cost of risk associated with possible accidents. The proposed model of this paper is used for a real-world case study of railroad transportation of Tehran region and the results have been analyzed.

  17. Dissecting aortic aneurysm in maintenance hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Ounissi M

    2009-01-01

    Full Text Available The dissecting aortic aneurysm (DAA is a rare pathology that may result in fatal outcome. We report follow up of three cases of DAA patients undergoing maintenance hemo-dialysis who were managed conservatively.

  18. Valves maintenance management in nuclear power plants

    International Nuclear Information System (INIS)

    Trost, J.

    2001-01-01

    The deregulation of Europe's power market will force nuclear power plant operators to introduce extensive cost-cutting measures in order to be able to compete within this new environment. The optimization of plant outages provides considerable potential for raising plant availability but can also lower operating costs by reducing expenditure on maintenance. Framatome ANP GmbH, in cooperation with plant operators is currently developing new and improved service concepts which can have a major effect on the way in which maintenance will be performed in the future. The concepts encompass optimization of the overall sequence from planning in advance to the individual measures including reduction of the scope of maintenance activities, identification of cost cutting potential and bundling of maintenance activities. (Author)

  19. Maintenance policy of the nuclear park

    International Nuclear Information System (INIS)

    Teisset, J.M.

    1995-01-01

    The nuclear installations maintenance policy of Electricite de France (EDF) follows three axes: first, controlling the aging of the production tool to warrant the safety and the functional reliability during the 40 years of installations service life. Second: optimizing the maintenance activities taking into account the safety goals, costs, security, dosimetry, duration of outages, and intervention conditions in the decisions. Third: developing a real partnership with contractors. EDF has decided to decentralize its maintenance operations towards each unit which is in charge of the organization of its own project. A better management of the contractor personnel has allowed to increase the performances of maintenance operations. The development of long-term agreements with contractors has permitted to reinforce the qualification, the responsibility and the efficiency of the personnel and to improve their means and tools. (J.S.). 1 photo

  20. Integrated sign management system : ADOT maintenance group

    Science.gov (United States)

    2003-12-01

    The Arizona Department of Transportation (ADOT) maintains and manages an inventory of roadway signs. Before the implementation of this project, sign technicians maintained inventory records on individual laptops to track their daily sign maintenance ...