WorldWideScience

Sample records for methods specifically productivity

  1. Electrospinning versus fibre production methods: from specifics to technological convergence.

    Science.gov (United States)

    Luo, C J; Stoyanov, Simeon D; Stride, E; Pelan, E; Edirisinghe, M

    2012-07-07

    Academic and industrial research on nanofibres is an area of increasing global interest, as seen in the continuously multiplying number of research papers and patents and the broadening range of chemical, medical, electrical and environmental applications. This in turn expands the size of the market opportunity and is reflected in the significant rise of entrepreneurial activities and investments in the field. Electrospinning is probably the most researched top-down method to form nanofibres from a remarkable range of organic and inorganic materials. It is well known and discussed in many comprehensive studies, so why this review? As we read about yet another "novel" method producing multifunctional nanomaterials in grams or milligrams in the laboratory, there is hardly any research addressing how these methods can be safely, consistently and cost-effectively up-scaled. Despite two decades of governmental and private investment, the productivity of nanofibre forming methods is still struggling to meet the increasing demand. This hinders the further integration of nanofibres into practical large-scale applications and limits current uses to niche-markets. Looking into history, this large gap between supply and demand of synthetic fibres was seen and addressed in conventional textile production a century ago. The remarkable achievement was accomplished via extensive collaborative research between academia and industry, applying ingenious solutions and technological convergence from polymer chemistry, physical chemistry, materials science and engineering disciplines. Looking into the present, current advances in electrospinning and nanofibre production are showing similar interdisciplinary technological convergence, and knowledge of industrial textile processing is being combined with new developments in nanofibre forming methods. Moreover, many important parameters in electrospinning and nanofibre spinning methods overlap parameters extensively studied in industrial

  2. Standardized methods for the production of high specific-activity zirconium-89

    Science.gov (United States)

    Holland, Jason P.; Sheh, Yiauchung; Lewis, Jason S.

    2009-01-01

    Zirconium-89 is an attractive metallo-radionuclide for use in immunoPET due to the favorable decay characteristics. Standardized methods for the routine production and isolation of high purity and high specific-activity 89Zr using a small cyclotron are reported. Optimized cyclotron conditions reveal high average yields of 1.52 ± 0.11 mCi/μA·h at a proton beam energy of 15 MeV and current of 15 μA using a solid, commercially available 89Y-foil target (0.1 mm, 100% natural abundance). 89Zr was isolated in high radionuclidic and radiochemical purity (>99.99%) as [89Zr]Zr-oxalate by using a solid-phase hydroxamate resin with >99.5% recovery of the radioactivity. The effective specific-activity of 89Zr was found to be in the range 5.28 – 13.43 mCi/μg (470 – 1195 Ci/mmol) of zirconium. New methods for the facile production of [89Zr]Zr-chloride are reported. Radiolabeling studies using the trihydroxamate ligand desferrioxamine B (DFO) gave 100% radiochemical yields in 7 days. Small-animal PET imaging studies have demonstrated that free 89Zr(IV) ions administered as [89Zr]Zr-chloride accumulate in the liver whilst [89Zr]Zr-DFO is excreted rapidly via the kidneys within <20 min. These results have important implication for the analysis of immunoPET imaging of 89Zr-labeled monoclonal antibodies. The detailed methods described can be easily translated to other radiochemistry facilities and will facilitate the use of 89Zr in both basic science and clinical investigations. PMID:19720285

  3. Identifying Industry-Specific Components of Product Liability Response System Using Delphi-AHP Method

    Directory of Open Access Journals (Sweden)

    Seo JunHyeok

    2016-12-01

    Full Text Available PL (product liability response system is an enterprise-wide system that prevents company’s financial loss due to PL-related accidents. Existing researches on PL response system are mainly focused on preventive and/or defense strategies for the companies. Also, it is obvious that each industry has their original characteristics related on PL issues. It means industry-specific characteristics should be considered to adopt PL response strategies. Thus, this paper aims to discuss industry-specific PL response system and their components. Based on prior researches, we tried to reveal the possibility of its application to manufacturing companies of existing PL response strategies using Delphi method with PL experts. Based on first round results, we tried to classify existing PL strategies of manufacturing companies into several categories. To validate our suggestion for essential components of PL response system, second round Delphi method are applied. Analytic hierarchy process (AHP technique will be applied to identify a prioritized list of each components and strategies. Existing PL response strategies could be categorized with six components – strategy, technology, investment, training, awareness, and organization. Among six components, Technology – it represents the technology needed for improving the safety of all products – is the most important components to prepare PL accidents. The limitation of this paper is on the size of survey and variety of examples. However, the future study will enhance the potential of the proposed method. Regardless of rich research efforts to identify PL response strategies, there is no effort to categorize these strategies and prioritized them. Well-coordinated and actionable PL response strategies and their priorities could help small-and-medium sized enterprise (SME to develop their own PL response system with their limited resources.

  4. Standard Test Method for Water Absorption, Bulk Density, Apparent Porosity, and Apparent Specific Gravity of Fired Whiteware Products

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2006-01-01

    1.1 This test method covers procedures for determining water absorption, bulk density, apparent porosity, and apparent specific gravity of fired unglazed whiteware products. 1.2 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

  5. Micro- and macroanalytical methods for the determination of the specific cumulated energy consumption for the production of consumer goods

    Energy Technology Data Exchange (ETDEWEB)

    Flaschar, W.

    1979-01-01

    As an estimation shows the total share of the final energy consumption for the production of goods amounts to more than 50%. Consequently, the present study is directed toward an important field. Apart from systemizing and confronting methods which have already been used it also tries to largely genereralize the problems of the specific cumulated energy consumption (SCEC). First, the terminolgy of energy and materials balances is fundamentally defined and determined. The influencing factors of the SCEC are analyzed and presented and the essential variables of energy consumption are explained with the help of examples. The fundamentals of cumulation as well as micro- and macroanalytical methods for the determination of the SCEC are worked out and discussed. The last part of the study shows the application of general methods and the solution of special problems when determining the SCEC for a particular product as exemplified by the practice of producing natural yogurt.

  6. Sika deer (Cervus nippon)-specific real-time PCR method to detect fraudulent labelling of meat and meat products.

    Science.gov (United States)

    Kaltenbrunner, Maria; Hochegger, Rupert; Cichna-Markl, Margit

    2018-05-08

    Since game meat is more valuable and expensive than meat from domesticated animal species it is a potential target for adulteration. Analytical methods must allow the identification and quantification of meat species to be applicable for the detection of fraudulent labelling. We developed a real-time PCR assay for the authentication of sika deer (Cervus nippon) and products thereof. The primer/probe system amplifies a 71 bp fragment of the kappa-casein precursor gene. Since the target sequence contained only one sika deer-specific base, we introduced a deliberate base mismatch in the forward primer. The real-time PCR assay did not show cross-reactivity with 19 animal and 49 plant species tested. Low cross-reactivity was observed with red deer, fallow deer, reindeer and moose. However, with a ΔCt value of ≥11.79 between sika deer and the cross-reacting species, cross-reactivity will not affect the accuracy of the method. LOD and LOQ, determined by analysing serial dilutions of a DNA extract containing 1% (w/w) sika deer DNA in pig DNA, were 0.3% and 0.5%, respectively. The accuracy was evaluated by analysing DNA mixtures and DNA isolates from meat extract mixtures and meat mixtures. In general, recoveries were in the range from 70 to 130%.

  7. Towards a theory of product design specifications

    DEFF Research Database (Denmark)

    Hansen, Claus Thorp; Andreasen, Mogens Myrup

    2004-01-01

    , which express the customers¿ need and perception of value. In this paper we will scrutinize this assumption. We outline the roles and tasks of the product design specification in order to identify the requirements on a theory of product design specifications and we identify existing theory elements....... In the design methodology literature we find guidelines and methods to compile a product design specification. The contributions are based on the common underlying assumption that it is meaningful and the only feasible approach to interpret the result of a need analysis into a set of technical specifications...... to build such a theory of product design specifications upon....

  8. Software specification methods

    CERN Document Server

    Habrias, Henri

    2010-01-01

    This title provides a clear overview of the main methods, and has a practical focus that allows the reader to apply their knowledge to real-life situations. The following are just some of the techniques covered: UML, Z, TLA+, SAZ, B, OMT, VHDL, Estelle, SDL and LOTOS.

  9. Spent Nuclear Fuel (SNF) Project Product Specification

    International Nuclear Information System (INIS)

    PAJUNEN, A.L.

    2000-01-01

    The process for removal of Spent Nuclear Fuel (SNF) from the K Basins has been divided into major sub-systems. The Fuel Retrieval System (FRS) removes fuel from the existing storage canisters, cleans it, and places it into baskets. The multi-canister overpack (MCO) loading system places the baskets into an MCO that has been pre-loaded in a cask. The cask, containing a loaded MCO, is then transferred to the Cold Vacuum Drying (CVD) Facility. After drying at the CVD Facility, the cask, and MCO, are transferred to the Canister Storage Building (CSB), where the MCO is removed from the cask, staged, inspected, sealed (by welding), and stored until a suitable permanent disposal option is implemented. The purpose of this document is to specify the process related characteristics of an MCO at the interface between major process systems. The characteristics are derived from the primary technical documents that form the basis for safety analysis and design calculations. This document translates the calculation assumptions into implementation requirements and describes the method of verifying that the requirement is achieved. These requirements are used to define validation test requirements and describe requirements that influence multiple sub-project safety analysis reports. This product specification establishes limits and controls for each significant process parameter at interfaces between major sub-systems that potentially affect the overall safety and/or quality of the SNF packaged for processing, transport, and interim dry storage. The product specifications in this document cover the SNF packaged in MCOs to be transported throughout the SNF Project. The description of the product specifications are organized in the document as follows: Section 2.0--Summary listing of product specifications at each major sub-system interface. Section 3.0--Summary description providing guidance as to how specifications are complied with by equipment design or processing within a major

  10. Spent Nuclear Fuel (SNF) Project Product Specification

    Energy Technology Data Exchange (ETDEWEB)

    PAJUNEN, A.L.

    2000-12-07

    The process for removal of Spent Nuclear Fuel (SNF) from the K Basins has been divided into major sub-systems. The Fuel Retrieval System (FRS) removes fuel from the existing storage canisters, cleans it, and places it into baskets. The multi-canister overpack (MCO) loading system places the baskets into an MCO that has been pre-loaded in a cask. The cask, containing a loaded MCO, is then transferred to the Cold Vacuum Drying (CVD) Facility. After drying at the CVD Facility, the cask, and MCO, are transferred to the Canister Storage Building (CSB), where the MCO is removed from the cask, staged, inspected, sealed (by welding), and stored until a suitable permanent disposal option is implemented. The purpose of this document is to specify the process related characteristics of an MCO at the interface between major process systems. The characteristics are derived from the primary technical documents that form the basis for safety analysis and design calculations. This document translates the calculation assumptions into implementation requirements and describes the method of verifying that the requirement is achieved. These requirements are used to define validation test requirements and describe requirements that influence multiple sub-project safety analysis reports. This product specification establishes limits and controls for each significant process parameter at interfaces between major sub-systems that potentially affect the overall safety and/or quality of the SNF packaged for processing, transport, and interim dry storage. The product specifications in this document cover the SNF packaged in MCOs to be transported throughout the SNF Project. The description of the product specifications are organized in the document as follows: Section 2.0--Summary listing of product specifications at each major sub-system interface. Section 3.0--Summary description providing guidance as to how specifications are complied with by equipment design or processing within a major

  11. Airing method-specific advertisements.

    Science.gov (United States)

    Yaser, Y

    1992-06-01

    The Turkish Family Health and Planning Foundation initiated the commercial marketing of contraceptives in 1989 as part of a Contraceptive Social Marketing (CSM) program to make available low-cost contraceptives. In 1988 modern methods were used by 31% and traditional methods by 32.3%, while 36.6% used no contraceptives. Only 6.2% were current pill users mainly because of health reasons since high-dose pills dominated the market. A 1990 survey among urban consumers indicated a 94% awareness of contraceptive methods, 76.1% of current use, and preference for the IUD. The side effects of the pill were cited for disliking it, and the condom was rated higher. The CSM project aims at popularizing low-dose pills by explaining the differences and benefits regarding high-dose pills. It collaborated with manufacturers: Schering, Wyeth, Organon, and Eczacibasi Ilac. In 1991 a TV and radio advertisement campaign started that involves the low-dose products Microgynon, Triquilar, Desolet, Lo-Ovral, and Tri-Nordial. The introduction of the Okey condom by Eczacibasi Ilac. In June 1991 also entailed extensive promotion with newspaper ads and TV spots after getting official permission. 1.3 million condoms were sold in the 1st 2 months in 13,000 retail outlets, and 4 million more were projected to be sold. A shift of the attitude of supermarket owners allowing stocking of condoms and the support of the Turkish Ministry of Health, USAID, and the Turkish Radio and Television Bureau has facilitated the CSM project implementation that will profoundly affect family planning in Turkey.

  12. WRAP 2A product specification

    International Nuclear Information System (INIS)

    Parker, K.E.

    1993-01-01

    WRAP-2A will process mixed and low-level waste (MLLW) for disposal. The final treatment processes selected for use in WRAP-2A consist of stabilization using cementitious materials and immobilization using thermosetting polymers. Modifications or additions to these processes may be made as technology improvements become known. Knowledge of the diverse waste forms that must be processed will be important to the effective exploration of process technologies that may be available. This document is a compilation of the current knowledge of the waste and process methods specified for each type of waste. As the uncertainties associated with the waste and methods of processing are addressed and resolved, revisions to this document will be made. This document is broken down by feed stream, source of the waste, waste codes, radiological characterization and recommended final forms of the waste for each stream

  13. Production of high specific activity silicon-32

    International Nuclear Information System (INIS)

    Phillips, D.R.; Brzezinski, M.A.

    1998-01-01

    This is the final report of a three-year, Laboratory Directed Research and Development Project (LDRD) at Los Alamos National Laboratory (LANL). There were two primary objectives for the work performed under this project. The first was to take advantage of capabilities and facilities at Los Alamos to produce the radionuclide 32 Si in unusually high specific activity. The second was to combine the radioanalytical expertise at Los Alamos with the expertise at the University of California to develop methods for the application of 32 Si in biological oceanographic research related to global climate modeling. The first objective was met by developing targetry for proton spallation production of 32 Si in KCl targets and chemistry for its recovery in very high specific activity. The second objective was met by developing a validated field-useable, radioanalytical technique, based upon gas-flow proportional counting, to measure the dynamics of silicon uptake by naturally occurring diatoms

  14. Product specifications for the end use

    International Nuclear Information System (INIS)

    Navaretta, G.

    1992-01-01

    This paper reports on the concept of total quality which provides a foundation upon which NGL specifications could be built. Total quality has been embraced by a large constituency within the Refining and Petrochemical business, so, the NGL industry can not afford to discount it. As a concept, total quality certainly includes a specific management approach, but the roots of the quality movement lie with traditional product quality values. Naturally, the subject of product quality leads directly to product specifications, thus proving the intimate relationship between the total quality concept and NGL specifications

  15. [The "specific" liability regime for blood products].

    Science.gov (United States)

    Byk, Christian

    2017-10-13

    Based on the system of liability for defective products as organized by the European Directive of 25 July 1985, responsibility for blood products does not therefore constitute a genuine specific regime. However, European law leaves States a margin of discretion in the implementation of the Directive with regard to health products. This is the case in particular with the exemption for development risk.

  16. Specific GMP guidelines for radiopharmaceutical products

    International Nuclear Information System (INIS)

    2000-01-01

    These guidelines are intended to complement those provided in ''Good manufacturing practices for pharmaceutical products'', as well as the GMP for sterile pharmaceutical products. The regulatory procedures necessary to control radiopharmaceutical products are in large part determined by the sources of products and methods of manufacture. Manufacturing procedures within the scope of these guidelines include: preparation of radiopharmaceuticals in hospital radiopharmacies, preparation of radiopharmaceuticals in centralized radiopharmacies, production of radiopharmaceuticals in nuclear centres, institutes or industrial manufacturers, preparation and production of radiopharmaceuticals in Positron Emission Tomography (PET) centres

  17. Asian product specifications to tighten by 2000

    International Nuclear Information System (INIS)

    Anon.

    1995-01-01

    The Asia/Pacific region is characterized by much diversity in environmental standards for petroleum products. Specifications in Japan, for example, are quite stringent, while, in India, kerosene--even that used for cooking or lighting--may contain as much as 0.2% sulfur. Widhyawan Prawiraatmadja and Fereidun Fesharaki of the East-West Center in Honolulu outlined regional product quality in a report titled, ''Trends in Petroleum Product Specifications in the Asia-Pacific Region: A Supply/Demand Analysis and Government Policy Responses.'' This paper summarizes the findings of this report with emphasis on gasoline, diesel fuels, and fuel oil demand, fuel consumption, and quality

  18. Spent nuclear fuel project product specification

    International Nuclear Information System (INIS)

    PAJUNEN, A.L.

    1999-01-01

    This document establishes the limits and controls for the significant parameters that could potentially affect the safety and/or quality of the Spent Nuclear Fuel (SNF) packaged for processing, transport, and storage. The product specifications in this document cover the SNF packaged in Multi-Canister Overpacks to be transported throughout the SNF Project

  19. Spent nuclear fuel project product specification

    International Nuclear Information System (INIS)

    Pajunen, A.L.

    1998-01-01

    Product specifications are limits and controls established for each significant parameter that potentially affects safety and/or quality of the Spent Nuclear Fuel (SNF) packaged for transport to dry storage. The product specifications in this document cover the spent fuel packaged in MultiCanister Overpacks (MCOs) to be transported throughout the SNF Project. The SNF includes N Reactor fuel and single-pass reactor fuel. The FRS removes the SNF from the storage canisters, cleans it, and places it into baskets. The MCO loading system places the baskets into MCO/Cask assembly packages. These packages are then transferred to the Cold Vacuum Drying (CVD) Facility. After drying at the CVD Facility, the MCO cask packages are transferred to the Canister Storage Building (CSB), where the MCOs are removed from the casks, staged, inspected, sealed (by welding), and stored until a suitable permanent disposal option is implemented. The key criteria necessary to achieve these goals are documented in this specification

  20. Hydrogen production methods

    International Nuclear Information System (INIS)

    Hammerli, M.

    1982-07-01

    Old, present and new proceses for producing hydrogen are assessed critically. The emphasis throughout is placed on those processes which could be commercially viable before the turn of the century for large-scale hydrogen manufacture. Electrolysis of water is the only industrial process not dependent on fossil resources for large-scale hydrogen production and is likely to remain so for the next two or three decades. While many new processes, including those utilizing sunlight directly or indirectly, are presently not considered to be commercially viable for large-scale hydrogen production, research and development effort is needed to enhance our understanding of the nature of these processes. Water vapour electrolysis is compared with thermochemical processes: the former has the potential for displacing all other processes for producing hydrogen and oxygen from water

  1. MODERN METHODS OF REASONABLE PRODUCT SUPPLY

    Directory of Open Access Journals (Sweden)

    Anna Kulik

    2016-11-01

    Full Text Available Thesis objective is to study modern methods of product supply with the purpose to determine optimal ways for their rationalization. Since the use of reasonable practices, taking into account external and internal factors under the specific conditions of product moving from the supplier to the buyer, makes the process of product supply economically viable, i.e., low costs for product transportation, ensures fast moving products, their safety and, ultimately, results in reduction of the costs of product disposal. Methodology. The study is based on theoretical methods to study this problem. System analysis method and simulation of the ways to improve were also used in the study. Results. Addressing these issues, the concept, form and stages of product supply process organization depending on the type of product have been studied; product supply management methods based on logistics concept of “demand response”. Practical significance. Optimization of the principles and methods of product supply, factors affecting its organization will, in practice, contribute to the development of reasonable product delivery systems featured with economic efficiency of advanced technologies of product supply. Value/ originality. The analyzed methods of product supply management based on logistics concept of “demand response” can ensure maximum reduction of response time to the changes in demand by rapid stocktaking at those points of the market where the demand is expected to increase, which will allow to reduce the costs of bringing the product to the consumer.

  2. Production of a broad specificity antibody for the development and validation of an optical SPR screening method for free and intracellular microcystins and nodularin in cyanobacteria cultures.

    Science.gov (United States)

    Devlin, Shauna; Meneely, Julie P; Greer, Brett; Campbell, Katrina; Vasconcelos, Vitor; Elliott, Christopher T

    2014-05-01

    A highly sensitive broad specificity monoclonal antibody was produced and characterised for microcystin detection through the development of a rapid surface plasmon resonance (SPR) optical biosensor based immunoassay. The antibody displayed the following cross-reactivity: MC-LR 100%; MC-RR 108%; MC-YR 68%; MC-LA 69%; MC-LW 71%; MC-LF 68%; and Nodularin 94%. Microcystin-LR was covalently attached to a CM5 chip and with the monoclonal antibody was employed in a competitive 4 min injection assay to detect total microcystins in water samples below the WHO recommended limit (1 µg/L). A 'total microcystin' level was determined by measuring free and intracellular concentrations in cyanobacterial culture samples as this toxin is an endotoxin. Glass bead beating was used to lyse the cells as a rapid extraction procedure. This method was validated according to European Commission Decision 96/23/EC criteria. The method was proven to measure intracellular microcystin levels, the main source of the toxin, which often goes undetected by other analytical procedures and is advantageous in that it can be used for the monitoring of blooms to provide an early warning of toxicity. It was shown to be repeatable and reproducible, with recoveries from spiked samples ranging from 74 to 123%, and had % CVs below 10% for intra-assay analysis and 15% for inter-assay analysis. The detection capability of the assay was calculated as 0.5 ng/mL for extracellular toxins and 0.05 ng/mL for intracellular microcystins. A comparison of the SPR method with LC-MS/MS was achieved by testing six Microcystis aeruginosa cultures and this study yielded a correlation R(2) value of 0.9989. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Coordiantion by using Product Specifications in Product Development

    DEFF Research Database (Denmark)

    Terkelsen, Søren Bendix

    1997-01-01

    This paper is based on a case study. It treats the coordination by generating product specifications in product development. This paper contains three very important aspects, which cause a need for coordination, and call attention to the coordination mechanisms. The three aspects are task...... uncertainty, task complexity, and dependencies between activities. If one want to select coordination mechanisms, which improve the performance in product development, it is very important to have a knowledge about these three aspects. In the following the aspects are identified in the literature...

  4. Production cost methods and data

    Science.gov (United States)

    Jeffe, R. E.; Fujita, T.

    1975-01-01

    The general gas cost equation for utility financing is presented. Modifications and assumptions made in order to apply the cost equation to hydrogen production are described. Cost data are given for various methods of hydrogen production. The cost matrix procedure is briefly discussed.

  5. Methodes for identification of specific language impairment

    Directory of Open Access Journals (Sweden)

    Toktam Maleki Shahmahmood

    2014-06-01

    Full Text Available Background and Aim: Specific language impiarment (SLI is one of the most prevalent developmental language disorders its diagnosis is a problematic issue among researchers and clinicians because of the heterogeneity of language profiles in the affected population and overlapping with other developmental language disorders. The aim of this study was to review the suggested diagnostic criteria for this disorder, controversies about these criteria and identify the most accurate diagnostic methods.Methods: Published article from 1980 to 2012 in bibliographic and publisher databases including Pubmed, Google scholar, Cochran library, Web of Science, ProQuest, Springer, Oxford, Science direct, Ovid, Iran Medex and Magiran about the diagnostic methods for discriminating preschoool children with specific language impiarment from normal developing children were reviewd in this article. These keywords were used for research: “specific language impairment”, “SLI”, “diagnosis or identification”, “standardized tests”, and “tests for language development”.Conclusion: The results of this study show inspite of agreement of researchers and clinicians about exclusionary criteria as one basic part of the diagnosis of specific language impiarment , there is no consensus about the other part, inclusionary criteria. Different studies used different inclusionary criteria which can be divided to categories of clincal judgment, discrepancy-based criteria, standardized testing, clinical markers and markers from spontaneous speech samples. Advantages, disadvantages, and clinical applicability of each diagnostic method are discussed in this article.

  6. [Study on commercial specification of atractylodes based on Delphi method].

    Science.gov (United States)

    Wang, Hao; Chen, Li-Xiao; Huang, Lu-Qi; Zhang, Tian-Tian; Li, Ying; Zheng, Yu-Guang

    2016-03-01

    This research adopts "Delphi method" to evaluate atractylodes traditional traits and rank correlation. By using methods of mathematical statistics the relationship of the traditional identification indicators and atractylodes goods rank correlation was analyzed, It is found that the main characteristics affectingatractylodes commodity specifications and grades of main characters wereoil points of transaction,color of transaction,color of surface,grain of transaction,texture of transaction andspoilage. The study points out that the original "seventy-six kinds of medicinal materials commodity specification standards of atractylodes differentiate commodity specification" is not in conformity with the actual market situation, we need to formulate corresponding atractylodes medicinal products specifications and grades.This study combined with experimental results "Delphi method" and the market actual situation, proposed the new draft atractylodes commodity specifications and grades, as the new atractylodes commodity specifications and grades standards. It provides a reference and theoretical basis. Copyright© by the Chinese Pharmaceutical Association.

  7. Meeting the challenges in product specifications

    International Nuclear Information System (INIS)

    Perez, A.

    1999-01-01

    The current state of the Canadian petroleum refining industry was discussed with special emphasis on the challenges the industry is facing regarding product specification and relevant public policy. In order to remain competitive and avoid the risk of losing much of its eastern and British Columbia markets to integrated North American supply alternatives, the refining industry has undergone severe restructuring, in the process closing 17 refineries in Canada since 1983. Although there has been an increased emphasis on lower cost feedstocks, processing has become more environmentally challenging. For example, the sulphur content in gasoline must meet stringent specifications. Air quality issues are pushing both vehicle technology and fuel formulation, and both vehicle and fuel manufacturers are looking for global solutions. Proposed Canadian regulations were described as not well geared to dealing with these solutions, i.e. fuel formulation standards are looked at in isolation from from each other, or from vehicle technology advancements. As an example the proposed regulation concerning sulphur in gasoline was cited, alleging that requiring lower levels two years in advance of the next generation of low emission vehicles will create a serious threat to the competitiveness of domestic refiners vis-a-vis their U.S. counterparts. 1 tab., 3 figs

  8. Savannah River Site production reactor technical specifications. K Production Reactor

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-02-01

    These technical specifications are explicit restrictions on the operation of the Savannah River Site K Production Reactor. They are designed to preserve the validity of the plant safety analysis by ensuring that the plant is operated within the required conditions bounded by the analysis, and with the operable equipment that is assumed to mitigate the consequences of an accident. Technical specifications preserve the primary success path relied upon to detect and respond to accidents. This report describes requirements on thermal-hydraulic limits; limiting conditions for operation and surveillance for the reactor, power distribution control, instrumentation, process water system, emergency cooling and emergency shutdown systems, confinement systems, plant systems, electrical systems, components handling, and special test exceptions; design features; and administrative controls.

  9. Optimisation of technical specifications using probabilistic methods

    International Nuclear Information System (INIS)

    Ericsson, G.; Knochenhauer, M.; Hultqvist, G.

    1986-01-01

    During the last few years the development of methods for modifying and optimising nuclear power plant Technical Specifications (TS) for plant operations has received increased attention. Probalistic methods in general, and the plant and system models of probabilistic safety assessment (PSA) in particular, seem to provide the most forceful tools for optimisation. This paper first gives some general comments on optimisation, identifying important parameters and then gives a description of recent Swedish experiences from the use of nuclear power plant PSA models and results for TS optimisation

  10. Specific methods on in-pile gammametry

    International Nuclear Information System (INIS)

    Pointud, M.-L.; Michel, Francois.

    1979-01-01

    The gammametry technique in nuclear research reactors has evolved by the adequation of its means and the quality of its results since its beginnings in 1972. We do not propose here to make a detailed presentation, nor describe the kinds of well known results that can henceforth be attained with it in a conventional manner; our intention, on the other hand, is to describe a few specific methods developed for using it in the SILOE reactor of the CEN/G [fr

  11. Methods and Production of Cementation Materials for Immobilisation into Waste Form. Research of Cementation Processes for Specific Liquid Radioactive Waste Streams of Radiochemical Plants

    International Nuclear Information System (INIS)

    Sukhanov, L.P.

    2013-01-01

    In the near future Russian Federation is planning to use industrial cementation facilities at two radiochemical combines - PA 'Mayak' and Mountain Chemical Combine. Scope of the research within the IAEA CRP contact No. 14176 included the development of cementation processes for specfic liquid radioactive waste streams that are present in these enterprisers. The research on cementation of liquid waste from spent nuclear fuel reprocessing at PA 'Mayak' allowed obtaining experimental data characterizing the technological process and basic characteristics of the produced cement compounds (e.g. mechanical strength, water resistance, frost resistance, flowability, etc.) immobilizing different streams of waste (e.g. hydrated-salt sludges, filter material pulps, mixture of hydrated salt slurries and filter material pulps, tritium liquid waste). Determined optimum technological parameters will allow industrial scale production of cement compound with required quality and higher flowability that is necessary for providing uniform filling of compartments of storage facilities at these sites. The research has been also carried out for the development of cementation technology for immobilization of pulps from storage tanks of Mountain Chemical Combine radiochemical plant. Cementation of such pulps is a difficult technological task because pulps are of complex chemical composition (e.g. hydroxides of manganese, iron, nickel, etc., as well as silicon oxide) and a relatively high activity. The research of cementation process selection for these pulps included studies of the impact of sorbing additive type and content on cement compounds leachability, flowability, impact of cement compound age to its mechanical strength, heat generation of cement compounds and others. The research results obtained allowed testing of cementation facility with a pulse type mixer on the full-scale. Use of such mixer for pulp cementation makes possible to prepare a homogeneous cement compound with the

  12. Transforming Multidisciplinary Customer Requirements to Product Design Specifications

    Science.gov (United States)

    Ma, Xiao-Jie; Ding, Guo-Fu; Qin, Sheng-Feng; Li, Rong; Yan, Kai-Yin; Xiao, Shou-Ne; Yang, Guang-Wu

    2017-09-01

    With the increasing of complexity of complex mechatronic products, it is necessary to involve multidisciplinary design teams, thus, the traditional customer requirements modeling for a single discipline team becomes difficult to be applied in a multidisciplinary team and project since team members with various disciplinary backgrounds may have different interpretations of the customers' requirements. A new synthesized multidisciplinary customer requirements modeling method is provided for obtaining and describing the common understanding of customer requirements (CRs) and more importantly transferring them into a detailed and accurate product design specifications (PDS) to interact with different team members effectively. A case study of designing a high speed train verifies the rationality and feasibility of the proposed multidisciplinary requirement modeling method for complex mechatronic product development. This proposed research offersthe instruction to realize the customer-driven personalized customization of complex mechatronic product.

  13. Some absolutely effective product methods

    Directory of Open Access Journals (Sweden)

    H. P. Dikshit

    1992-01-01

    Full Text Available It is proved that the product method A(C,1, where (C,1 is the Cesàro arithmetic mean matrix, is totally effective under certain conditions concerning the matrix A. This general result is applied to study absolute Nörlund summability of Fourier series and other related series.

  14. Method of storing solidification products

    International Nuclear Information System (INIS)

    Tani, Yutaro.

    1985-01-01

    Purpose: To enable to efficiently and satisfactorily cool and store solidification products of liquid wastes generated from the reactor spent fuel reprocessing process by a simple facility. Method: Liquid wastes generated from the reactor spent fuel reprocessing process are caused to flow from the upper opening to the inside of a spherical canistor. The opening of the spherical canistor is welded with a lid by a remote control and the liquid wastes are tightly sealed within the spherical canistor as glass solidification products. Spherical canistors having the solidification products tightly sealed therein are sent into and stored in a hopper by the remote control. Further, a blower is driven upon storing to suck cooling air from the cooling air intake port to the inside of the hopper to absorb the decay heat of radioactive materials in the solidification products and the air is discharged from the duct and through the stack to the atmosphere. (Kawakami, Y.)

  15. Accelerator Production and Separations for High Specific Activity Rhenium-186

    Energy Technology Data Exchange (ETDEWEB)

    Jurisson, Silvia S. [Univ. of Missouri, Columbia, MO (United States); Wilbur, D. Scott [Univ. of Washington, Seattle, WA (United States)

    2016-04-01

    Tungsten and osmium targets were evaluated for the production of high specific activity rhenium-186. Rhenium-186 has potential applications in radiotherapy for the treatment of a variety of diseases, including targeting with monoclonal antibodies and peptides. Methods were evaluated using tungsten metal, tungsten dioxide, tungsten disulfide and osmium disulfide. Separation of the rhenium-186 produced and recycling of the enriched tungsten-186 and osmium-189 enriched targets were developed.

  16. Specific-structured lipids: nutritional perspectives and production potentials

    DEFF Research Database (Denmark)

    Xu, Xuebing; Høy, Carl-Erik; Balchen, Steen

    1997-01-01

    Structured lipids are referring to any triacylglycerols containing both long chain fatty acids (mostly essential fatty acids) and medium or short chain fatty acids. In case of specific-structured lipids (SSLs), each group of fatty acids locates specifically at sn-2 or -1.3 positions of the glycerol...... backbone. Recently the nutritional perspectives of this kind of lipids attract many interests. This causes an increasing interest in the production of them by lipase-catalyzed interesterification. One of the advantages of lipase method over chemical ones is that SSLs can be produced with particular fatty...

  17. Method of burning petrochemical products

    Energy Technology Data Exchange (ETDEWEB)

    Sado, I

    1973-01-12

    This invention concerns a method of burning wastes such as polyvinyl chloride or other synthetic resin products and rubbers, in which wastes are burned in a nearly smokeless and odorless state. The method is characterized by a process by which petrochemical waste products are subjected to a spontaneous combustion in a casserole state in a closed combustion room in such a way that no air is supplied whatever, and subsequently the gas so generated is sent successively in an adequate amount into a separately installed second combustion room where it is reburnt at a high temperature of more than 1000 C by a jet flame from the oil burners mounted inside the combustion room. Usually, petrochemical products emanate black smoke of Ringelmann concentration of more than five and a strong odor, but in this method, particularly in the case of polyvinyl chloride the exhaust smoke has a Ringelmann smoke concentration of less than one and is almost odorless because the plastic is completely gasified by the spontaneous combustion and completely burned at 1300 to 1400/sup 0/C with oil and air in the second combustion room. When the exhaust smoke is passed through a neutralization tank to remove the chloride compounds in the smoke, the damaging contribution of the exhaust gas or smoke to the secondary pollution can be completely eliminated.

  18. Containers and Packaging: Product-Specific Data

    Science.gov (United States)

    This web page provide numbers on the different containers and packaging products in our municipal solid waste. These include containers of all types, such as glass, steel, plastic, aluminum, wood, and other types of packaging

  19. Production of Monoclonal Antibodies specific for Progesterone

    OpenAIRE

    YÜCEL, Fatıma

    2014-01-01

    Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H1...

  20. Exotic nuclei: production, properties and specificity

    International Nuclear Information System (INIS)

    Van Duppen, Piet

    1998-01-01

    In this paper we will put the production of energetic radioactive ion beams of exotic nuclei in perspective and describe the two complementary production processes that are used nowadays: Isotope Separation On-Line (ISOL) followed by post-acceleration, and In-Flight Separation (IFS). After a general description of the process we will focus on recent technical developments in the field. In the subsequent section we give some 'typical' examples of physics cases that are addressed with exotic beams. The examples are chosen not only because their physics importance but also to demonstrate the complementary aspects in the production process and the detection systems. This overview will not be complete and the reader is referred to literature for further information, nor is the lecture intended to give an overview of all the efforts that are taking place in the field by mentioning every project separately. (author)

  1. A method of hydrogen production

    International Nuclear Information System (INIS)

    Schulten, R.; Teggers, H.; Schulze-Bentrop, R.

    1975-01-01

    This method of producing hydrogen from water in a multistage cycle process works without anorganic salts and requires only gases and liquids. Carbon oxide is catalytically converted into carbon dioxide and water by means of water vapour. The carbon dioxide is then converted into sulphuric acid and carbon oxide using water and sulphur dioxide at high temperatures and pressures, and the sulphuric acid is separated into sulphur dioxide, oxygen and water via the intermediate SO 2 . The SO 2 and CO 2 thus obtained are led back into the appropriate reaction stages, and hydrogen and oxygen are removed from the process as end products. (A schematic flow diagram is given.) (UWI) [de

  2. [Production, specificity and structure of immunoglobulins].

    Science.gov (United States)

    Goujard, C; Delfraissy, J F

    1991-03-21

    Immunoglobulin is a key factor of the immune response resulting from B-cell activation and associated with T-cell stimulation. Because of its structure, this antibody has a dual function: it specifically recognizes the inducer antigen in the variable region and eliminates it by a constant portion which is responsible for effector properties. Surface immunoglobulin, therefore, is the B-cell antigen receptor; it differs from the T-cell receptor in that it recognizes the antigen unbound to the major istocompatibility complex; binding the antigen results in direct signal transduction first in the cytoplasm, then in the nucleus. This receptor can be secreted in the body: it is made up of circulating immunoglobulins. Human immunoglobulins are divided into 5 classes, each of them with its own response kinetics, distribution and functions. The variability of the antibody response accounts for a genetic organization involving numerous genes which may be associated with each other, or mutate, or recombine during maturation of the lymphocytes. Altogether, this system has a theoretical capacity of response to three hundred million different antigens.

  3. Formal specification level concepts, methods, and algorithms

    CERN Document Server

    Soeken, Mathias

    2015-01-01

    This book introduces a new level of abstraction that closes the gap between the textual specification of embedded systems and the executable model at the Electronic System Level (ESL). Readers will be enabled to operate at this new, Formal Specification Level (FSL), using models which not only allow significant verification tasks in this early stage of the design flow, but also can be extracted semi-automatically from the textual specification in an interactive manner.  The authors explain how to use these verification tasks to check conceptual properties, e.g. whether requirements are in conflict, as well as dynamic behavior, in terms of execution traces. • Serves as a single-source reference to a new level of abstraction for embedded systems, known as the Formal Specification Level (FSL); • Provides a variety of use cases which can be adapted to readers’ specific design flows; • Includes a comprehensive illustration of Natural Language Processing (NLP) techniques, along with examples of how to i...

  4. Specific activity measurement of 64Cu: A comparison of methods

    International Nuclear Information System (INIS)

    Mastren, Tara; Guthrie, James; Eisenbeis, Paul; Voller, Tom; Mebrahtu, Efrem; Robertson, J. David; Lapi, Suzanne E.

    2014-01-01

    Effective specific activity of 64 Cu (amount of radioactivity per µmol metal) is important in order to determine purity of a particular 64 Cu lot and to assist in optimization of the purification process. Metal impurities can affect effective specific activity and therefore it is important to have a simple method that can measure trace amounts of metals. This work shows that ion chromatography (IC) yields similar results to ICP mass spectrometry for copper, nickel and iron contaminants in 64 Cu production solutions. - Highlights: • Comparison of TETA titration, ICP mass spectrometry, and ion chromatography to measure specific activity. • Validates ion chromatography by using ICP mass spectrometry as the “gold standard”. • Shows different types and amounts of metal impurities present in 64 Cu

  5. Product specific emissions from municipal solid waste landfills

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Exner, Stephan; Jørgensen, Anne-Mette

    1998-01-01

    This paper presents and verifies the computer tool LCA-LAND for estimation of emissions from specific waste products disposed in municipal solid waste landfills in European countries for use in the inventory analysis of LCA. Examples of input data (e.g. distribution of the waste product...... in different countries, composition of the product and physical/chemical/biological properties of waste product components) and output data (e.g. estimated emissions to atmosphere and water) are given for a fictive waste product made of representative types of components (toluene, cellulose, polyvinylchloride...... (PVC), copper and chloride). Since waste products from different processes in the product system may be disposed at different landfills where they are mixed with waste originating outside the product system, the estimated emissions from specific waste products cannot be compared with measured emissions...

  6. PCMO L01-Setting Specifications for Biological Investigational Medicinal Products.

    Science.gov (United States)

    Krause, Stephan O

    2015-01-01

    This paper provides overall guidance and best practices for the setting of specifications for clinical biological drug substances and drug products within the framework of ICH guidelines on pharmaceutical development [Q8(R2) and Q11], quality risk management (Q9), and quality systems (Q10). A review is provided of the current regulatory expectations for the specification setting process as part of a control strategy during product development, pointing to existing challenges for the investigational new drug/investigational medicinal product dossier (IND/IMPD) sponsor. A case study illustrates how the investigational medicinal product specification revision process can be managed within a flexible quality system, and how specifications can be set and justified for early and late development stages. This paper provides an overview for the setting of product specifications for investigational medicinal products used in clinical trials. A case study illustrates how product specifications of investigational medicinal products can be justified and managed within a modern product quality system. © PDA, Inc. 2015.

  7. Specific decontamination methods: water nozzle, cavitation erosion

    International Nuclear Information System (INIS)

    Boulitrop, D.; Gauchon, J.P.; Lecoffre, Y.

    1984-05-01

    The erosion and decontamination tests carried out in the framework of this study, allowed to specify the fields favourable to the use of the high pressure jet taking into account the determinant parameters that are the pressure and the target-nozzle distance. The previous spraying of gels with chemical reagents (sulfuric acid anf hydrazine) allows to get better decontamination factors. Then, the feasibility study of a decontamination method by cavitation erosion is presented. Gelled compounds for decontamination have been developed; their decontamination quality has been evaluated by comparative contamination tests in laboratory and decontamination tests of samples of materials used in nuclear industry; this last method is adapted to remote handling devices and produces a low quantity of secondary effluents, so it allows to clean high contaminated installation on the site without additional exposure of the personnel [fr

  8. Determination of impurities and degradation products from veterinary medicinal products by HPLC method

    Directory of Open Access Journals (Sweden)

    Elena Gabriela Oltean

    2014-06-01

    Full Text Available The organic or inorganic impurities in the veterinary medicinal product can derive from starting materials, manufacturing process, incomplete purification, inappropriate storage. The acceptable levels of impurities in pharmaceuticals are estimated by comparison with standard solutions, according to the appropriate monographs. Forced degradation studies determine the stability of the method of dosage for the active compounds and for the entire finished product under excessive accelerated degradation conditions. They also provide information on degradation pathways and selectivity of analytical methods applied. The information provided by the degradation studies on the active compound and finished pharmaceutical product should demonstrate the specificity of the analytical method regarding impurities. Forced degradation studies should demonstrate that the impurities and degradation products generated do not interfere with the active compound. The current forced degradation methods consist of acid hydrolysis, basic hydrolysis, oxidation, exposure of the medicinal product to temperature and light. HPLC methods are an integral analytical instrument for the analysis of the medicinal product. The HPLC method should be able to separate, detect and quantify various specific degradation products that can appear after manufacture or storage of the medicinal product, as well as new elements appearing after synthesis. FDA and ICH guidelines recommend the enclosure of the results, including the chromatograms specific to the forced degradation-subjected medicinal product, in the documentation for marketing authorization. Using HPLC methods in forced degradation studies on medicinal products provides relevant information on the method of determination for the formulation of the medicinal product, synthesis product, packaging methods and storage.

  9. Structuring Formal Requirements Specifications for Reuse and Product Families

    Science.gov (United States)

    Heimdahl, Mats P. E.

    2001-01-01

    In this project we have investigated how formal specifications should be structured to allow for requirements reuse, product family engineering, and ease of requirements change, The contributions of this work include (1) a requirements specification methodology specifically targeted for critical avionics applications, (2) guidelines for how to structure state-based specifications to facilitate ease of change and reuse, and (3) examples from the avionics domain demonstrating the proposed approach.

  10. Application Protocol, Initial Graphics Exchange Specification (IGES), Layered Electrical Product

    Energy Technology Data Exchange (ETDEWEB)

    O`Connell, L.J. [ed.

    1994-12-01

    An application protocol is an information systems engineering view of a specific product The view represents an agreement on the generic activities needed to design and fabricate the product the agreement on the information needed to support those activities, and the specific constructs of a product data standard for use in transferring some or all of the information required. This application protocol describes the data for electrical and electronic products in terms of a product description standard called the Initial Graphics Exchange Specification (IGES). More specifically, the Layered Electrical Product IGES Application Protocol (AP) specifies the mechanisms for defining and exchanging computer-models and their associated data for those products which have been designed in two dimensional geometry so as to be produced as a series of layers in IGES format The AP defines the appropriateness of the data items for describing the geometry of the various parts of a product (shape and location), the connectivity, and the processing and material characteristics. Excluded is the behavioral requirements which the product was intended to satisfy, except as those requirements have been recorded as design rules or product testing requirements.

  11. ASPECTS REGARDING NEUROMARKETING SPECIFIC RESEARCH METHODS

    Directory of Open Access Journals (Sweden)

    MIRELA-CRISTINA VOICU

    2012-05-01

    Full Text Available Information is one of the most important resources that a company must posses. Some informations are hidden deep in the black box - the mind of the consumer. Neuromarketing helps us find what is inside of the black box without troubling the consumer with questions that he doesn’t want to answer or that he can’t answer. Today because of an extensive research in mapping cortical and subcortical activity in association with behaviors and thoughts, the confidence in neurological data is growing. Thanks to discoveries in perceptual sciences we can identify the parts of the brain that are responsible for the phenomena that we experience daily. For all those interested in information obtained through neuromarketing techniques it becomes evident that there are corresponding neural substrates of consumer decision making process and these substrates can be observed, measured, and possibly manipulated. The following paper reveals some important aspects of the use of neuromarketing in studying consumer behavior by presenting the concepts, methods and techniques used under this sophisticated name, the limitations and advantages of using neuromarketing techniques and the importance of this type of information in decision making process at a company level.

  12. Product specific emissions from municipal solid waste landfills

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Hauschild, Michael Zwicky

    1998-01-01

    For the inventory analysis of environmental impacts associated with products in LCA there is a great need for estimates of emissions from waste products disposed at municipal solid waste landfills (product specific emissions). Since product specific emissions can not be calculated or measured...... directly at the landfills, they must be estimated by modelling of landfill processes. This paper presents a landfill model based on a large number of assumptions and approximations concerning landfill properties, waste product properties and characteristics of various kinds of environmental protection...... systems (e.g. landfill gas combustion units and leachate treatment units). The model is useful for estimation of emissions from waste products disposed in landfills and it has been made operational in the computer tool LCA-LAND presented in a following paper. In the model, waste products are subdivided...

  13. Maintenance Approaches for Different Production Methods

    Directory of Open Access Journals (Sweden)

    Mungani, Dzivhuluwani Simon

    2013-11-01

    Full Text Available Various production methods are used in industry to manufacture or produce a variety of products needed by industry and consumers. The nature of a product determines which production method is most suitable or cost-effective. A continuous process is typically used to produce large volumes of liquids or gases. Batch processing is often used for small volumes, such as pharmaceutical products. This paper discusses a research project to determine the relationship between maintenance approaches and production methods. A survey was done to determine to what extent three maintenance approaches reliability-centred maintenance (RCM, total productive maintenance (TPM, and business-centred maintenance (BCM are used for three different processing methods (continuous process, batch process, and a production line method.

  14. Determination production costs using PBC method

    Directory of Open Access Journals (Sweden)

    Todić Vladimir V.

    2014-01-01

    Full Text Available Basic characteristics of modern markets make requirements in quality increasing, decreasing prices and shortening delivery of products. In the middle of this requirements are production costs for whose determination are developed many traditional and alternative methods including PBC method (Process Based Costing. This method enables precisely locating and calculating indirect production costs, and with determined direct costs enables determination of total production costs. This paper shows usage of PBC method for determination production costs for three forms of processing cutting tools.

  15. Extending product modeling methods for integrated product development

    DEFF Research Database (Denmark)

    Bonev, Martin; Wörösch, Michael; Hauksdóttir, Dagný

    2013-01-01

    Despite great efforts within the modeling domain, the majority of methods often address the uncommon design situation of an original product development. However, studies illustrate that development tasks are predominantly related to redesigning, improving, and extending already existing products...... and PVM methods, in a presented Product Requirement Development model some of the individual drawbacks of each method could be overcome. Based on the UML standard, the model enables the representation of complex hierarchical relationships in a generic product model. At the same time it uses matrix....... Updated design requirements have then to be made explicit and mapped against the existing product architecture. In this paper, existing methods are adapted and extended through linking updated requirements to suitable product models. By combining several established modeling techniques, such as the DSM...

  16. 19 CFR 134.43 - Methods of marking specific articles.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Methods of marking specific articles. 134.43...; DEPARTMENT OF THE TREASURY COUNTRY OF ORIGIN MARKING Method and Location of Marking Imported Articles § 134.43 Methods of marking specific articles. (a) Marking previously required by certain provisions of the...

  17. Nuclear data for specific problems. Part 1: Methods

    International Nuclear Information System (INIS)

    Leszczynski, Francisco

    1999-01-01

    The growing volume of basic nuclear data, methods and codes for processing these data, and the wide variety of problems where these data and codes are required, oblige to have an efficient system for managing all this information. In this work we present a new methodology for nuclear data processing, applied to neutron and photon transport calculations for specific problems. The base of the new methodology is the analysis of the requirements, following the chain: Problem-Components-Materials-Elements-Isotopes-Process-Tests-Final product (a library with processed data). This order is the inverse of the normal order followed up to date where, for performing a specific calculation, the first step is the choice of an existing data library for general purposes, without the previous steps of pre-processing data, and tests of the final library. Then, the used data are limited to the isotope content of this library, and the adaptation of material compositions and components to the data availability is necessary , performing finally the required calculations in a rather approximated form, depending on the available data. An interactive computer program for PC , is developed, for managing all the information generated by nuclear data processing, with the additional advantage of having a help tool for performing the needed analysis, before processing data calculations for specific applications. These analyses are based on the particular characteristics of each application, and the processed information of previous cases, is stored in conveniently designed data bases for an easy inspection of its contents. By means of an example of application of the new method, in this paper the methods of analysis and calculations and the tools used (computer programs, data bases and documents) are describes. (author)

  18. Method and computer program product for maintenance and modernization backlogging

    Science.gov (United States)

    Mattimore, Bernard G; Reynolds, Paul E; Farrell, Jill M

    2013-02-19

    According to one embodiment, a computer program product for determining future facility conditions includes a computer readable medium having computer readable program code stored therein. The computer readable program code includes computer readable program code for calculating a time period specific maintenance cost, for calculating a time period specific modernization factor, and for calculating a time period specific backlog factor. Future facility conditions equal the time period specific maintenance cost plus the time period specific modernization factor plus the time period specific backlog factor. In another embodiment, a computer-implemented method for calculating future facility conditions includes calculating a time period specific maintenance cost, calculating a time period specific modernization factor, and calculating a time period specific backlog factor. Future facility conditions equal the time period specific maintenance cost plus the time period specific modernization factor plus the time period specific backlog factor. Other embodiments are also presented.

  19. Advances in product family and product platform design methods & applications

    CERN Document Server

    Jiao, Jianxin; Siddique, Zahed; Hölttä-Otto, Katja

    2014-01-01

    Advances in Product Family and Product Platform Design: Methods & Applications highlights recent advances that have been made to support product family and product platform design and successful applications in industry. This book provides not only motivation for product family and product platform design—the “why” and “when” of platforming—but also methods and tools to support the design and development of families of products based on shared platforms—the “what”, “how”, and “where” of platforming. It begins with an overview of recent product family design research to introduce readers to the breadth of the topic and progresses to more detailed topics and design theory to help designers, engineers, and project managers plan, architect, and implement platform-based product development strategies in their companies. This book also: Presents state-of-the-art methods and tools for product family and product platform design Adopts an integrated, systems view on product family and pro...

  20. Production method of α particles

    International Nuclear Information System (INIS)

    Prevot, F.

    1953-01-01

    It is proposed a method to get an intense beam of α particles. With a source of ordinary ions, we form a helium beam, once ionized, it is accelerated with an energy of a few hundreds of keV. While crossing a matter any that can be a thin leaf or a gaseous blade, the second electron of helium is pulled with a yield that only depends on the energy of the beam of helium and that is equal to 1/2 for 650 keV. (author) [fr

  1. SPECIFIC FEATURES OF DEVELOPMENT OF ORGANIC PRODUCTS MARKET IN UKRAINE

    Directory of Open Access Journals (Sweden)

    T. Kharchenko

    2013-08-01

    Full Text Available The article is dedicated to the development of new and improvement of existing theoretical and methodological basis of forming and developing the market of organic products, its correspondence to the present-day situation, determination of problems and ways of their solving, introduction in practical activity of Ukrainian enterprises. The main objective of the article is to determine the specific features of forming and developing organic products market in Ukraine, and the perspective directions of its development based on analysis and practice of functioning of such markets in the world. The environmentally sound products market in the world is being analyzed, some information on the countries with the most commodity turnover of organic products, structure of international market of organic products, volumes of sales of organic products in the European countries is provided. As a result of studying the modern trends of economic development the authors reach a conclusion on problems of standard introduction, investigate the European norms and requirements for organic products. The conducted research allows distinguishing the basic features of Ukrainian market of organic products: it quickly grows, which makes it especially appealing for the participants of market relations, however entry into this market requires considerable capital investments and is characterized by high risk; criteria for qualifying products as environmentally sound products are unstructured and unclear. The potential for growth of organic products market in Ukraine is examined.

  2. Permanent burying method for product

    International Nuclear Information System (INIS)

    Sakai, Goro; Sakata, Noboru; Hironaka, Yoshikazu; Shigematsu, Kazuo; Yurugi, Masahiro; Minami, Masayoshi; Yoshisaki, Masato.

    1995-01-01

    In a method of permanently burying an object by filling and solidifying a cement mortar in gaps between each of objects to be buried underground, cement mortar is filled into gaps, which comprises water at a unit amount determined as from 200 to 250kg/m 3 , a cement at low water/cement ratio (%) of from 70 to 400%, and contains fine powder having an average grain size of not greater than 100μm (not containing cement) of 50 to 800kg/m 3 , fine aggregates of 800 to 1200kg/m 3 , UERAN gum (a bio-gum powder produced by aerobic fermentation of alcaligenes-bacteria) of 20g/m 3 to 1.3kg/m 3 , a dispersing agent of 0 to 40kg/m 3 , a swelling agent of 0 to 40kg/m 3 . Then if the mortar blended with the UERAN gum is injected, any gaps can be filled tightly, no breeding is caused and since the amount of cement is small, it does not suffer from temperature cracking. Therefore, the state of filling is kept permanently, and environmental pollution caused by radioactive wastes can be prevented. (N.H.)

  3. Plasma processing methods for hydrogen production

    International Nuclear Information System (INIS)

    Mizeraczyk, J.; Jasinski, M.

    2016-01-01

    In the future a transfer from the fossil fuel-based economy to hydrogen-based economy is expected. Therefore the development of systems for efficient H_2 production becomes important. The several conventional methods of mass-scale (or central) H_2 production (methane, natural gas and higher hydrocarbons reforming, coal gasification reforming) are well developed and their costs of H_2 production are acceptable. However, due to the H_2 transport and storage problems the small-scale (distributed) technologies for H_2 production are demanded. However, these new technologies have to meet the requirement of producing H_2 at a production cost of $(1-2)/kg(H_2) (or 60 g(H_2)/kWh) by 2020 (the U.S. Department of Energy's target). Recently several plasma methods have been proposed for the small-scale H_2 production. The most promising plasmas for this purpose seems to be those generated by gliding, plasmatron and nozzle arcs, and microwave discharges. In this paper plasma methods proposed for H_2 production are briefly described and critically evaluated from the view point of H_2 production efficiency. The paper is aiming at answering a question if any plasma method for the small-scale H_2 production approaches such challenges as the production energy yield of 60 g(H_2)/kWh, high production rate, high reliability and low investment cost. (authors)

  4. Composition and methods for improved fuel production

    Science.gov (United States)

    Steele, Philip H.; Tanneru, Sathishkumar; Gajjela, Sanjeev K.

    2015-12-29

    Certain embodiments of the present invention are configured to produce boiler and transportation fuels. A first phase of the method may include oxidation and/or hyper-acidification of bio-oil to produce an intermediate product. A second phase of the method may include catalytic deoxygenation, esterification, or olefination/esterification of the intermediate product under pressurized syngas. The composition of the resulting product--e.g., a boiler fuel--produced by these methods may be used directly or further upgraded to a transportation fuel. Certain embodiments of the present invention also include catalytic compositions configured for use in the method embodiments.

  5. An Intelligent Method of Product Scheme Design Based on Product Gene

    Directory of Open Access Journals (Sweden)

    Qing Song Ai

    2013-01-01

    Full Text Available Nowadays, in order to have some featured products, many customers tend to buy customized products instead of buying common ones in supermarket. The manufacturing enterprises, with the purpose of improving their competitiveness, are focusing on providing customized products with high quality and low cost as well. At present, how to produce customized products rapidly and cheaply has been the key challenge to manufacturing enterprises. In this paper, an intelligent modeling approach applied to supporting the modeling of customized products is proposed, which may improve the efficiency during the product design process. Specifically, the product gene (PG method, which is an analogy of biological evolution in engineering area, is employed to model products in a new way. Based on product gene, we focus on the intelligent modeling method to generate product schemes rapidly and automatically. The process of our research includes three steps: (1 develop a product gene model for customized products; (2 find the obtainment and storage method for product gene; and (3 propose a specific genetic algorithm used for calculating the solution of customized product and generating new product schemes. Finally, a case study is applied to test the usefulness of our study.

  6. Artefact specification, design, and production as a process of communication

    DEFF Research Database (Denmark)

    Galle, Per

    1999-01-01

    about communication as literal ‘transmission’ or ‘sharing’ of ideas. It is then explained how the initial artefact specification, the design representation, and ultimately the artefact itself can all be seen as symbolic expressions of ideas that are communicated, and it is shown how the conditions...... for the success of communication carry over to the artefact production process. The purpose of the analysis is to provide a conceptual background for the study of various aspects of artefact production, such as design, or the development of computer-based tools for collaborative design, product modelling...

  7. Production of high specific activity 123I for protein iodination for medical use

    International Nuclear Information System (INIS)

    Legoux, Y.; Cieur, M.; Crouzel, C.; Syrota, A.

    1985-01-01

    Iodine-123 is produced via xenon-133 by irradiation of a sodium iodide target with 108 MeV deuterons from the synchrocyclotron of IPN. The on-line production method is described. The specific activity of the iodine is determined by neutron activation analysis and by a radioimmunological method. The conditions labelling different proteins (insulin, angiotensin) are given and also the purification method to obtain a product ready for injection to patients. (author)

  8. Production of high specific activity /sup 123/I for protein iodination for medical use

    Energy Technology Data Exchange (ETDEWEB)

    Legoux, Y; Cieur, M [Paris-11 Univ., 91 - Orsay (France). Inst. de Physique Nucleaire; Goutheraud, R; Drouet, J [Centre National de Transfusion Sanguine, 75 - Paris (France); Crouzel, C; Syrota, A [CEA, 91 - Orsay (France). Service Hospitalier Frederic Joliot

    1985-01-01

    Iodine-123 is produced via xenon-133 by irradiation of a sodium iodide target with 108 MeV deuterons from the synchrocyclotron of IPN. The on-line production method is described. The specific activity of the iodine is determined by neutron activation analysis and by a radioimmunological method. The conditions labelling different proteins (insulin, angiotensin) are given and also the purification method to obtain a product ready for injection to patients.

  9. Authentication of gold products by nuclear methods

    International Nuclear Information System (INIS)

    De Jesus, A.S.M.

    1985-01-01

    The falsification of valuable gold items is a threat to the authenticity of gold products. To solve this, there is a continuous search for reliable, practicle and cost-effective means of identifying forgeries. Because nuclear techniques as applied to elemental analysis have a high degree of specificity, are non-destructive and permit the availability of results within a relatively short time, a few of these techniques were investigated and reviewed in the article. Work on some promising methods in the author's laboratory is also discussed. Constraints such as those imposed by the time taken by the measurement, negligible residual activity within a relatively short time were also considered. The techniques that were investigated include: the transmission of electromagnetic radiation through a medium; scattering of electromagnetic radiation; x-ray fluorescence analysis; neutron activation analysis; activation by the inelastic scattering of gamma radiation; activation by the inelastic scattering of fast neutrons; absorption and scattering of fast neutrons; self-attenuation of gamma radiation. The shape of the object being investigated, should also be considered. It is concluded that a system based on the inelastic scattering of neutrons emitted by a 241 Am/Be source (halflife = 433 years) is practical and capable of authenticating gold and gold alloy coins such as Krugerrands. The feasibility study on the assaying of gold jewelry by means of nuclear methods also showed it to be impractical

  10. Methods and materials for production of terpenoids

    DEFF Research Database (Denmark)

    2015-01-01

    The invention provide novel methods for production of terpenoids. The methods take advantage of combinations of enzymes, which are not present in nature. Thus the methods involves use of host organisms expressing a diTPS of class II, diTPS of class I, and one or more CYPs....

  11. Investigation of change in specifications during a product's lifecycle

    DEFF Research Database (Denmark)

    Sudin, Mohd Nizam Bin; Ahmed, Saeema

    2009-01-01

    design attributes likely be changed; the initiation of change and how change in specifications are described. For this purpose, document analysis for a complex product has been carried out. In total, 271 reports of change request of an aero-engine that were associated to change in specifications were......Engineering changes (ECs) constitutes a normal part of a product’s lifecycle. This paper aims to understand why changes are made to a product’s specification during a product’s lifecycle including understanding: the distribution of changes; the drivers for changes; how changes are discovered; which...... the request was initiated. The study showed that experience plays a vital role in discovering the need to change a complex product...

  12. Calculation of radiation production of high specific activity isotopes 192Ir and 60Co

    International Nuclear Information System (INIS)

    Zhou Quan; Zhong Wenfa; Xu Xiaolin

    1997-01-01

    The high specific activity isotopes: 192 Ir and 60 Co in the high neutron flux reactor are calculated with the method of reactor physics. The results of calculation are analyzed in two aspects: the production of isotopes and the influence to parameters of the reactor, and hence a better case is proposed as a reference to the production

  13. Auxiliary BE Production by African American English-Speaking Children with and without Specific Language Impairment

    Science.gov (United States)

    Garrity, April W.; Oetting, Janna B.

    2010-01-01

    Purpose: To examine 3 forms ("am," "is," "are") of auxiliary BE production by African American English (AAE)-speaking children with and without specific language impairment (SLI). Method: Thirty AAE speakers participated: 10 six-year-olds with SLI, 10 age-matched controls, and 10 language-matched controls. BE production was examined through…

  14. Machine Learning Methods for Production Cases Analysis

    Science.gov (United States)

    Mokrova, Nataliya V.; Mokrov, Alexander M.; Safonova, Alexandra V.; Vishnyakov, Igor V.

    2018-03-01

    Approach to analysis of events occurring during the production process were proposed. Described machine learning system is able to solve classification tasks related to production control and hazard identification at an early stage. Descriptors of the internal production network data were used for training and testing of applied models. k-Nearest Neighbors and Random forest methods were used to illustrate and analyze proposed solution. The quality of the developed classifiers was estimated using standard statistical metrics, such as precision, recall and accuracy.

  15. OPTIMIZATION METHODS IN TRANSPORTATION OF FOREST PRODUCTS

    Directory of Open Access Journals (Sweden)

    Selçuk Gümüş

    2008-04-01

    Full Text Available Turkey has total of 21.2 million ha (27 % forest land. In this area, average 9 million m3 of logs and 5 million stere of fuel wood have been annually produced by the government forest enterprises. The total annual production is approximately 13million m3 Considering the fact that the costs of transporting forest products was about . 160 million TL in the year of 2006, the importance of optimizing the total costs in transportation can be better understood. Today, there is not common optimization method used at whole transportation problems. However, the decision makers select the most appropriate methods according to their aims.Comprehending of features and capacity of optimization methods is important for selecting of the most appropriate method. The evaluation of optimization methods that can be used at forest products transportation is aimed in this study.

  16. System and method for deriving a process-based specification

    Science.gov (United States)

    Hinchey, Michael Gerard (Inventor); Rash, James Larry (Inventor); Rouff, Christopher A. (Inventor)

    2009-01-01

    A system and method for deriving a process-based specification for a system is disclosed. The process-based specification is mathematically inferred from a trace-based specification. The trace-based specification is derived from a non-empty set of traces or natural language scenarios. The process-based specification is mathematically equivalent to the trace-based specification. Code is generated, if applicable, from the process-based specification. A process, or phases of a process, using the features disclosed can be reversed and repeated to allow for an interactive development and modification of legacy systems. The process is applicable to any class of system, including, but not limited to, biological and physical systems, electrical and electro-mechanical systems in addition to software, hardware and hybrid hardware-software systems.

  17. Nanomedicinal products: a survey on specific toxicity and side effects

    Directory of Open Access Journals (Sweden)

    Brand W

    2017-08-01

    Full Text Available Walter Brand,1,* Cornelle W Noorlander,1,* Christina Giannakou,2,3 Wim H De Jong,2 Myrna W Kooi,1 Margriet VDZ Park,2 Rob J Vandebriel,2 Irene EM Bosselaers,4 Joep HG Scholl,5 Robert E Geertsma2 1Centre for Safety of Substances and Products, 2Centre for Health Protection, National Institute for Public Health and the Environment (RIVM, Bilthoven, 3Department of Toxicogenomics, Maastricht University, Maastricht, 4Section Pharmacology, Toxicology and Pharmacokinetics, Medicines Evaluation Board (CBG-MEB, Utrecht, 5Research & Analysis Department, Netherlands Pharmacovigilance Centre Lareb, ‘s-Hertogenbosch, the Netherlands *These authors contributed equally to this work Abstract: Due to their specific properties and pharmacokinetics, nanomedicinal products (NMPs may present different toxicity and side effects compared to non-nanoformulated, conventional medicines. To facilitate the safety assessment of NMPs, we aimed to gain insight into toxic effects specific for NMPs by systematically analyzing the available toxicity data on approved NMPs in the European Union. In addition, by comparing five sets of products with the same active pharmaceutical ingredient (API in a conventional formulation versus a nanoformulation, we aimed to identify any side effects specific for the nano aspect of NMPs. The objective was to investigate whether specific toxicity could be related to certain structural types of NMPs and whether a nanoformulation of an API altered the nature of side effects of the product in humans compared to a conventional formulation. The survey of toxicity data did not reveal nanospecific toxicity that could be related to certain types of structures of NMPs, other than those reported previously in relation to accumulation of iron nanoparticles (NPs. However, given the limited data for some of the product groups or toxicological end points in the analysis, conclusions with regard to (a lack of potential nanomedicine-specific effects need to be

  18. Method for anaerobic fermentation and biogas production

    DEFF Research Database (Denmark)

    2013-01-01

    The present invention relates to a method for biomass processing, anaerobic fermentation of the processed biomass, and the production biogas. In particular, the invention relates to a system and method for generating biogas from anaerobic fermentation of processed organic material that comprises...

  19. Method for the production of primary amines

    NARCIS (Netherlands)

    Baldenius, Kai-Uwe; Ditrich, Klaus; Breurer, Michael; Navickas, Vaidotas; Janssen, Dick; Crismaru, Ciprian; Bartsch, Sebastian

    2014-01-01

    The present invention relates to a novel enzymatically catalyzed method for the production of aliphatic primary amines, which method comprises the enzymatic oxidation of a primary aliphatic alcohol catalyzed by an alcohol dehydrogenase, amination of the resulting oxocompound catalyzed by a

  20. Development of a multiplex polymerase chain reaction-sequence-specific primer method for NKG2D and NKG2F single-nucleotide polymorphism typing using isothermal multiple displacement amplification products.

    Science.gov (United States)

    Kaewmanee, M; Phoksawat, W; Romphruk, A; Romphruk, A V; Jumnainsong, A; Leelayuwat, C

    2013-06-01

    Natural killer group 2 member D (NKG2D) on immune effector cells recognizes multiple stress-inducible ligands. NKG2D single-nucleotide polymorphism (SNP) haplotypes were related to the levels of cytotoxic activity of peripheral blood mononuclear cells. Indeed, these polymorphisms were also located in NKG2F. Isothermal multiple displacement amplification (IMDA) is used for whole genome amplification (WGA) that can amplify very small genomic DNA templates into microgram with whole genome coverage. This is particularly useful in the cases of limited amount of valuable DNA samples requiring multi-locus genotyping. In this study, we evaluated the quality and applicability of IMDA to genetic studies in terms of sensitivity, efficiency of IMDA re-amplification and stability of IMDA products. The smallest amount of DNA to be effectively amplified by IMDA was 200 pg yielding final DNA of approximately 16 µg within 1.5 h. IMDA could be re-amplified only once (second round of amplification), and could be kept for 5 months at 4°C and more than a year at -20°C without loosing genome coverage. The amplified products were used successfully to setup a multiplex polymerase chain reaction-sequence-specific primer for SNP typing of the NKG2D/F genes. The NKG2D/F multiplex polymerase chain reaction (PCR) contained six PCR mixtures for detecting 10 selected SNPs, including 8 NKG2D/F SNP haplotypes and 2 additional NKG2D coding SNPs. This typing procedure will be applicable in both clinical and research laboratories. Thus, our data provide useful information and limitations for utilization of genome-wide amplification using IMDA and its application for multiplex NKG2D/F typing. © 2013 John Wiley & Sons Ltd.

  1. Methods of checking general safety criteria in UML statechart specifications

    International Nuclear Information System (INIS)

    Pap, Zsigmond; Majzik, Istvan; Pataricza, Andras; Szegi, Andras

    2005-01-01

    This paper describes methods and tools for safety analysis of UML statechart specifications. A comprehensive set of general safety criteria including completeness and consistency is applied in automated analysis. Analysis techniques are based on OCL expressions, graph transformations and reachability analysis. Two canonical intermediate representations of the statechart specification are introduced. They are suitable for straightforward implementation of checker methods and for the support of the proof of the correctness and soundness of the applied analysis. One of them also serves as a basis of the metamodel of a variant of UML statecharts proposed for the specification of safety-critical control systems. The analysis is extended to object-oriented specifications. Examples illustrate the application of the checker methods implemented by an automated tool-set

  2. The research on business rules classification and specification methods

    OpenAIRE

    Baltrušaitis, Egidijus

    2005-01-01

    The work is based on the research of business rules classification and specification methods. The basics of business rules approach are discussed. The most common business rules classification and modeling methods are analyzed. Business rules modeling techniques and tools for supporting them in the information systems are presented. Basing on the analysis results business rules classification method is proposed. Templates for every business rule type are presented. Business rules structuring ...

  3. QUANTIFYING SUPPLIERS’ PRODUCT QUALITY: AN EXPLORATORY PRODUCT AUDIT METHOD

    Directory of Open Access Journals (Sweden)

    S. Avakh Darestani

    2012-01-01

    Full Text Available

    ENGLISH ABSTRACT:The quality of the raw material and supplied product from suppliers plays a critical role in the quality of the final product. It has become the norm that vehicle manufacturers require their suppliers to measure product quality and service with a product audit method. Measuring quality of product is emphasised by QS9000 VDA6.5 and ISO/TS16949. From a competitive standpoint, and also to see continuous improvement in business, companies need to monitor their suppliers’ performance. Quality and delivery are two very important indicators of supplier performance. This paper presents a statistical method for measuring the quality of supplied product. This method allocates different weights to variables and attributes characteristics. Moreover, following normal distribution, the tolerance zone is divided to three regions with different scores. Therefore, the quality of suppliers’ products can be monitored based on the Product Quality Audit Score (PQAS. However, this method may be employed for organisations to monitor their raw material, work-in-process parts, and final product. It can be an indicator to monitor supplier quality behaviour.

    AFRIKAANSE OPSOMMING: Die gehalte van grondstowwe en produkte/komponente wat deur leweransiers verskaf word, speel ‘n kritiese rol in die gehalte van die finale produk. Dit het die norm geword in die motorvervaardigingsbedryf dat daar van leweransiers verwag word om hulle produkkwaliteit en –diens te meet by wyse van ‘n produkouditmetode. Die meting van produkkwaliteit word benadruk deur QS9000 VDA6.5 en ISO/TS16949. Uit ‘n mededingingshoek en ook om kontinue verbetering te monitor, is dit noodsaaklik dat leweransiers se verrigting gemeet word. Gehalte en aflewering is twee van die belangrikste indikatore van leweransiersverrigting. In hierdie artikel word ‘n statistiese model voorgehou vir die meting van die kwaliteit van die gelewerde produk. Die metode ken verskillende

  4. Methods for high yield production of terpenes

    Energy Technology Data Exchange (ETDEWEB)

    Kutchan, Toni; Higashi, Yasuhiro; Feng, Xiaohong

    2017-01-03

    Provided are enhanced high yield production systems for producing terpenes in plants via the expression of fusion proteins comprising various combinations of geranyl diphosphate synthase large and small subunits and limonene synthases. Also provided are engineered oilseed plants that accumulate monoterpene and sesquiterpene hydrocarbons in their seeds, as well as methods for producing such plants, providing a system for rapidly engineering oilseed crop production platforms for terpene-based biofuels.

  5. Improving developer productivity with C++ embedded domain specific languages

    Science.gov (United States)

    Kozacik, Stephen; Chao, Evenie; Paolini, Aaron; Bonnett, James; Kelmelis, Eric

    2017-05-01

    Domain-specific languages are a useful tool for productivity allowing domain experts to program using familiar concepts and vocabulary while benefiting from performance choices made by computing experts. Embedding the domain specific language into an existing language allows easy interoperability with non-domain-specific code and use of standard compilers and build systems. In C++, this is enabled through the template and preprocessor features. C++ embedded domain specific languages (EDSLs) allow the user to write simple, safe, performant, domain specific code that has access to all the low-level functionality that C and C++ offer as well as the diverse set of libraries available in the C/C++ ecosystem. In this paper, we will discuss several tools available for building EDSLs in C++ and show examples of projects successfully leveraging EDSLs. Modern C++ has added many useful new features to the language which we have leveraged to further extend the capability of EDSLs. At EM Photonics, we have used EDSLs to allow developers to transparently benefit from using high performance computing (HPC) hardware. We will show ways EDSLs combine with existing technologies and EM Photonics high performance tools and libraries to produce clean, short, high performance code in ways that were not previously possible.

  6. Tumor specific glycoproteins and method for detecting tumorigenic cancers

    International Nuclear Information System (INIS)

    Davidson, E.A.; Bolmer, S.D.

    1981-01-01

    The detection of tumour specific glycoproteins (TSGP) in human sera often indicates the presence of a malignant tumour in a patient. The distinguishing characteristics of TSGP isolated from the blood sera of cancer patients are described in detail together with methods of TSGP isolation and purification. Details are also given of radioimmunoassay techniques capable of detecting very low levels of serum TSGP with high specificity. (U.K.)

  7. A convenient method to synthesize specifically labelled cholesterol with tritium

    International Nuclear Information System (INIS)

    Malik, S.; Kenny, M.; Ahmad, S.; Washington Univ., Seattle, WA

    1992-01-01

    A simple method is described to label cholesterol with tritium. Cholesterol was first oxidized to 5-cholesten-3-one which was then purified by HPLC. Its structure was established by electron impact (EI) mass spectrometry and 1 H-NMR spectroscopy. The ketone was reduced with NaB 3 H 4 to give specifically labelled cholesterol (C-3 3 H) at low specific activity. (author)

  8. Hybrid Lanczos-type product methods

    Energy Technology Data Exchange (ETDEWEB)

    Ressel, K.J. [Swiss Center for Scientific Computing, Zuerich (Switzerland)

    1996-12-31

    A general framework is proposed to construct hybrid iterative methods for the solution of large nonsymmetric systems of linear equations. This framework is based on Lanczos-type product methods, whose iteration polynomial consists of the Lanczos polynomial multiplied by some other arbitrary, {open_quotes}shadow{close_quotes} polynomial. By using for the shadow polynomial Chebyshev (more general Faber) polynomials or L{sup 2}-optimal polynomials, hybrid (Chebyshev-like) methods are incorporated into Lanczos-type product methods. In addition, to acquire spectral information on the system matrix, which is required for such a choice of shadow polynomials, the Lanczos-process can be employed either directly or in an QMR-like approach. The QMR like approach allows the cheap computation of the roots of the B-orthogonal polynomials and the residual polynomials associated with the QMR iteration. These roots can be used as a good approximation for the spectrum of the system matrix. Different choices for the shadow polynomials and their construction are analyzed. The resulting hybrid methods are compared with standard Lanczos-type product methods, like BiOStab, BiOStab({ell}) and BiOS.

  9. Foundation, and also method for its production

    NARCIS (Netherlands)

    Katsma, H.

    2001-01-01

    Foundation, and method for the production of such a foundation. It is proposed that it be based on prefabricated concrete elements. The elements consist of base elements (2) and end elements (4). Each element is provided with two sockets for receiving pretensioning cables (6), which sockets are

  10. SPECIFIC METHOD OF RISK ASSESSMENT IN TOURISM ENTERPRISES

    Directory of Open Access Journals (Sweden)

    Andreea ARMEAN

    2014-12-01

    Full Text Available The objective of this paper is to present an innovative method of risk assessment for tourism businesses. The contribution to literature is the novelty of this method of following paths: is an ante-factum assessment not post-factum; risk assessment is based on perception rather than results; is based on specific risks tourism enterprises not on the overall risks. Is an asset-research methodology and consists in generating its own method of risk assessment based on the ideas summarized from the literature studied. The aim established is tourism enterprises from Romania. The data necessary for the application of this method will result from applying to top level management of tourism enterprises, a questionnaire about risk perception. The results from this study will help identify and measure the risks specific to tourism enterprises. The applicability of the results is to improve risk management in these enterprises.

  11. Analysis Method for Integrating Components of Product

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Jun Ho [Inzest Co. Ltd, Seoul (Korea, Republic of); Lee, Kun Sang [Kookmin Univ., Seoul (Korea, Republic of)

    2017-04-15

    This paper presents some of the methods used to incorporate the parts constituting a product. A new relation function concept and its structure are introduced to analyze the relationships of component parts. This relation function has three types of information, which can be used to establish a relation function structure. The relation function structure of the analysis criteria was established to analyze and present the data. The priority components determined by the analysis criteria can be integrated. The analysis criteria were divided based on their number and orientation, as well as their direct or indirect characteristic feature. This paper presents a design algorithm for component integration. This algorithm was applied to actual products, and the components inside the product were integrated. Therefore, the proposed algorithm was used to conduct research to improve the brake discs for bicycles. As a result, an improved product similar to the related function structure was actually created.

  12. Method of reprocessing radioactive asphalt solidification products

    International Nuclear Information System (INIS)

    Nakaya, Iwao; Murakami, Tadashi; Miyake, Takafumi; Inagaki, Yuzo.

    1986-01-01

    Purpose: To obtain heat-stable solidification products and decrease the total volume thereof by modifying the solidified form by the reprocessing of existent radioactive asphalt solidification products. Method: Radioactive asphalt solidification products are heated into a fluidized state. Then, incombustible solvents such as perchloroethylene or trichloroethylene are added to a dissolving tank to gradually dissolve the radioactive asphalt solidification products. Thus, organic materials such as asphalts are transferred into the solvent layer, while inorganic materials containing radioactive materials remain as they are in the separation tank. Then, the inorganic materials containing the radioactive materials are taken out and then solidified, for example, by converting them into a rock or glass form. (Kawakami, Y.)

  13. Analysis Method for Integrating Components of Product

    International Nuclear Information System (INIS)

    Choi, Jun Ho; Lee, Kun Sang

    2017-01-01

    This paper presents some of the methods used to incorporate the parts constituting a product. A new relation function concept and its structure are introduced to analyze the relationships of component parts. This relation function has three types of information, which can be used to establish a relation function structure. The relation function structure of the analysis criteria was established to analyze and present the data. The priority components determined by the analysis criteria can be integrated. The analysis criteria were divided based on their number and orientation, as well as their direct or indirect characteristic feature. This paper presents a design algorithm for component integration. This algorithm was applied to actual products, and the components inside the product were integrated. Therefore, the proposed algorithm was used to conduct research to improve the brake discs for bicycles. As a result, an improved product similar to the related function structure was actually created.

  14. PULP OBTAINING METHOD FOR PACKAGE PRODUCTION

    Directory of Open Access Journals (Sweden)

    V. V. Kuzmich

    2015-01-01

    Full Text Available The paper presents a new method for obtaining pulp which is used for production of cardboard, paper and package while using carbon dioxide and hydrazine hydrate and neutral-sulfite  shive cooking. Output increase of  the desired product can be explained by reduction in destruction of plant raw material carbohydrates during its cooking process. Quality improvement of the desired product (improvement in bleaching and output is attributed to the fact that usage of carbon dioxide and hydrazine contributes to provision of polysaccharide chain resistance to destruction due to the presence of  end links having structure of metasaccharinic and aldonic acids.The author has developed a new method for pulp obtaining on the basis of the executed investigations  and literature data.  СО2 and hydrazine hydrate have been used for obtaining pulp. Method invention concerns pulp obtaining and it can be used for paper and cardboard package manufacturing in pulp and paper industry.The method is to be carried in the following way: pulp-containing plant raw material is loaded into an autoclave and then aqua solution of sodium monosulfite containing hydrazine hydrate that constitutes 4–5 % of absolute dry pulp-containing raw material mass with liquid module 1:6–1:8 is supplied into the autoclave. The autoclave is closed for operation under pressure and the solution is carbonated under pressure which constitutes 5–8 % of absolute dry plant raw material (shover. Temperature is subsequently raised up to 180 °С in the space of 2 hours and cooking is carried out in the course of 4 hours. Usage of  the proposed method for shover cooking makes it possible to reduce monosulfite cooking process and improve qualitative characteristics and output of the desired product.  In addition to above mentioned fact there is a possibility to improve bleaching and final product output. 

  15. Specific surface area evaluation method by using scanning electron microscopy

    International Nuclear Information System (INIS)

    Petrescu, Camelia; Petrescu, Cristian; Axinte, Adrian

    2000-01-01

    Ceramics are among the most interesting materials for a large category of applications, including both industry and health. Among the characteristic of the ceramic materials, the specific surface area is often difficult to evaluate.The paper presents a method of evaluation for the specific surface area of two ceramic powders by means of scanning electron microscopy measurements and an original method of computing the specific surface area.Cumulative curves are used to calculate the specific surface area under assumption that the values of particles diameters follow a normal logarithmic distribution. For two powder types, X7R and NPO the results are the following: - for the density ρ (g/cm 2 ), 5.5 and 6.0, respectively; - for the average diameter D bar (μm), 0.51 and 0.53, respectively; - for σ, 1.465 and 1.385, respectively; - for specific surface area (m 2 /g), 1.248 and 1.330, respectively. The obtained results are in good agreement with the values measured by conventional methods. (authors)

  16. A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method.

    Science.gov (United States)

    Karkhane, Ali Asghar; Yakhchali, Bagher; Rastgar Jazii, Ferdous; Bambai, Bijan; Aminzadeh, Saeed; Rahimi, Fatemeh

    2015-06-01

    Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein's structure and function. We introduced a nested-SOE-PCR (N -SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR. Genomic DNA from Bacillus thermocatenulatus was extracted. Nested PCR was used to amplify B. thermocatenulatus lipase gene variants, namely wild type and mutant, using gene specific and mutagenic specific primers, followed by cloning in a suitable vector. Briefly in N-SOE-PCR method, instead of two pairs of primers, three pairs of primers are used to amplify a mutagenic fragment. Moreover, the first and second PCR products are slightly longer than PCR products in a conventional SOE. PCR products obtained from the first round of PCR are used for the second PCR by applying the nested and mutated primers. Following to the purification of the amplified fragments, they will be subject of the further purification and will be used as template to perform the third round of PCR using gene specific primers. In the end, the products will be cloned into a suitable vector for subsequent application. In comparison to the conventional SOE-PCR, the improved method (i.e. N-SOE-PCR) increases the yield and specificity of the products. In addition, the proposed method shows a large reduction in the non-specific products. By applying two more primers in the conventional SOE, the specificity of the method will be improved. This would be in part due to annealing of the primers further inside the amplicon that increases both the efficiency and a better attachment of the primers. Positioning of the primer far from both ends of an amplicon leads to an enhanced binding as well as increased affinity in the third round of amplification in SOE.

  17. Compliance uncertainty of diameter characteristic in the next-generation geometrical product specifications and verification

    International Nuclear Information System (INIS)

    Lu, W L; Jiang, X; Liu, X J; Xu, Z G

    2008-01-01

    Compliance uncertainty is one of the most important elements in the next-generation geometrical product specifications and verification (GPS). It consists of specification uncertainty, method uncertainty and implementation uncertainty, which are three of the four fundamental uncertainties in the next-generation GPS. This paper analyzes the key factors that influence compliance uncertainty and then proposes a procedure to manage the compliance uncertainty. A general model on evaluation of compliance uncertainty has been devised and a specific formula for diameter characteristic has been derived based on this general model. The case study was conducted and it revealed that the completeness of currently dominant diameter characteristic specification needs to be improved

  18. Time-trends in method-specific suicide rates compared with the availability of specific compounds

    DEFF Research Database (Denmark)

    Nordentoft, Merete; Qin, Ping; Helweg-Larsen, Karin

    2006-01-01

    Restriction of means for suicide is an important part of suicide preventive strategies in different countries. All suicides in Denmark between 1970 and 2000 were examined with regard to method used for suicide. Overall suicide mortality and method-specific suicide mortality was compared...... in the number of suicides by self-poisoning with these compounds. Restricted access occurred concomittantly with a 55% decrease in suicide rate....

  19. Specific radiological monitoring (SRM) in oil and gas production platforms

    International Nuclear Information System (INIS)

    Hairul Nizam Idris, Syed Asraf Fahlawi Wafa S.M Ghazi and Fadzley Izwan Abd Manaf

    2007-01-01

    Technologically enhanced naturally occurring radioactive materials (TENORM) are present in components of both oil and natural gas production facilities. TENORM can be associated with the presence of crude oil, produced water and natural gas. The radiation exposure pathways to the workers in oil and gas production are similar to those in the uranium and heavy mineral sand mining and processing industry. This paper work provides a short review on the Specific Radiological Monitoring (SRM) program were carried out at oil and gas platforms in the east cost of Peninsular Malaysia. The objective of this paper work is to observe the monitoring parameters levels and to evaluate whether these levels are exceeding the limits set by Atomic Energy Licensing Board (AELB). The monitoring results showed that the surface contamination, airborne contamination and concentration of radon and thoron are well below the set limit stipulated in LEM/TEK/30 SEM.2, except for external radiation and radioactivity concentration of sludge and scales. About 2 (2.35%) from the 85 external radiation measurements performed were found above the permissible limit. While about 11 (36.6%) and 7 (23.3%) of the 30 collected sludge and scales samples were found containing higher Ra-226 and Ra-228, respectively, than the mean concentrations in normal soils of Peninsular Malaysia. In general, it can be concluded that a few of oil and gas production platform are producing TENORM. (Author)

  20. Chemical Methods for Peptide and Protein Production

    Directory of Open Access Journals (Sweden)

    Istvan Toth

    2013-04-01

    Full Text Available Since the invention of solid phase synthetic methods by Merrifield in 1963, the number of research groups focusing on peptide synthesis has grown exponentially. However, the original step-by-step synthesis had limitations: the purity of the final product decreased with the number of coupling steps. After the development of Boc and Fmoc protecting groups, novel amino acid protecting groups and new techniques were introduced to provide high quality and quantity peptide products. Fragment condensation was a popular method for peptide production in the 1980s, but unfortunately the rate of racemization and reaction difficulties proved less than ideal. Kent and co-workers revolutionized peptide coupling by introducing the chemoselective reaction of unprotected peptides, called native chemical ligation. Subsequently, research has focused on the development of novel ligating techniques including the famous click reaction, ligation of peptide hydrazides, and the recently reported a-ketoacid-hydroxylamine ligations with 5-oxaproline. Several companies have been formed all over the world to prepare high quality Good Manufacturing Practice peptide products on a multi-kilogram scale. This review describes the advances in peptide chemistry including the variety of synthetic peptide methods currently available and the broad application of peptides in medicinal chemistry.

  1. Chemical methods for peptide and protein production.

    Science.gov (United States)

    Chandrudu, Saranya; Simerska, Pavla; Toth, Istvan

    2013-04-12

    Since the invention of solid phase synthetic methods by Merrifield in 1963, the number of research groups focusing on peptide synthesis has grown exponentially. However, the original step-by-step synthesis had limitations: the purity of the final product decreased with the number of coupling steps. After the development of Boc and Fmoc protecting groups, novel amino acid protecting groups and new techniques were introduced to provide high quality and quantity peptide products. Fragment condensation was a popular method for peptide production in the 1980s, but unfortunately the rate of racemization and reaction difficulties proved less than ideal. Kent and co-workers revolutionized peptide coupling by introducing the chemoselective reaction of unprotected peptides, called native chemical ligation. Subsequently, research has focused on the development of novel ligating techniques including the famous click reaction, ligation of peptide hydrazides, and the recently reported α-ketoacid-hydroxylamine ligations with 5-oxaproline. Several companies have been formed all over the world to prepare high quality Good Manufacturing Practice peptide products on a multi-kilogram scale. This review describes the advances in peptide chemistry including the variety of synthetic peptide methods currently available and the broad application of peptides in medicinal chemistry.

  2. Production and Processing of Subject-Verb Agreement in Monolingual Dutch Children with Specific Language Impairment

    Science.gov (United States)

    Blom, Elma; Vasic, Nada; de Jong, Jan

    2014-01-01

    Purpose: In this study, the authors investigated whether errors with subject-verb agreement in monolingual Dutch children with specific language impairment (SLI) are influenced by verb phonology. In addition, the productive and receptive abilities of Dutch acquiring children with SLI regarding agreement inflection were compared. Method: An SLI…

  3. Prospects for the methods of radionuclide production

    International Nuclear Information System (INIS)

    Karamyan, S.A.; Dmitriev, S.N.

    2014-01-01

    Methods of radionuclide production for the nuclear-medicine purposes are described. In a budget approach, the application of low-energy accelerators is especially advantageous. Intense flux of bremsstrahlung at electron accelerators or high-current cyclotron beams of alpha particles must supply a great yield for many isotopes. The choice of a target material and of the projectile energy provides enough variation for concrete species formation. The innovating procedures are here proposed for optimizing of methods, for instance, application of the noble-gas target for production and transport of activities. The known and new variants of the 'generator' scheme are discussed. Many isotopes are listed as promising in the context of the therapeutic and theragnostic applications. Among them are isotopes/isomers emitting soft radiation for the selective and careful body treatment, also the positron emitters for PET, and the halogen and alkali-metal species convenient for chemical separation.

  4. Using non-parametric methods in econometric production analysis

    DEFF Research Database (Denmark)

    Czekaj, Tomasz Gerard; Henningsen, Arne

    2012-01-01

    by investigating the relationship between the elasticity of scale and the farm size. We use a balanced panel data set of 371~specialised crop farms for the years 2004-2007. A non-parametric specification test shows that neither the Cobb-Douglas function nor the Translog function are consistent with the "true......Econometric estimation of production functions is one of the most common methods in applied economic production analysis. These studies usually apply parametric estimation techniques, which obligate the researcher to specify a functional form of the production function of which the Cobb...... parameter estimates, but also in biased measures which are derived from the parameters, such as elasticities. Therefore, we propose to use non-parametric econometric methods. First, these can be applied to verify the functional form used in parametric production analysis. Second, they can be directly used...

  5. Reactor and method for production of nanostructures

    Science.gov (United States)

    Sunkara, Mahendra Kumar; Kim, Jeong H.; Kumar, Vivekanand

    2017-04-25

    A reactor and method for production of nanostructures, including metal oxide nanowires or nanoparticles, are provided. The reactor includes a regulated metal powder delivery system in communication with a dielectric tube; a plasma-forming gas inlet, whereby a plasma-forming gas is delivered substantially longitudinally into the dielectric tube; a sheath gas inlet, whereby a sheath gas is delivered into the dielectric tube; and a microwave energy generator coupled to the dielectric tube, whereby microwave energy is delivered into a plasma-forming gas. The method for producing nanostructures includes providing a reactor to form nanostructures and collecting the formed nanostructures, optionally from a filter located downstream of the dielectric tube.

  6. Novel Method of Production Decline Analysis

    Science.gov (United States)

    Xie, Shan; Lan, Yifei; He, Lei; Jiao, Yang; Wu, Yong

    2018-02-01

    ARPS decline curves is the most commonly used in oil and gas field due to its minimal data requirements and ease application. And prediction of production decline which is based on ARPS analysis rely on known decline type. However, when coefficient index are very approximate under different decline type, it is difficult to directly recognize decline trend of matched curves. Due to difficulties above, based on simulation results of multi-factor response experiments, a new dynamic decline prediction model is introduced with using multiple linear regression of influence factors. First of all, according to study of effect factors of production decline, interaction experimental schemes are designed. Based on simulated results, annual decline rate is predicted by decline model. Moreover, the new method is applied in A gas filed of Ordos Basin as example to illustrate reliability. The result commit that the new model can directly predict decline tendency without needing recognize decline style. From arithmetic aspect, it also take advantage of high veracity. Finally, the new method improves the evaluation method of gas well production decline in low permeability gas reservoir, which also provides technical support for further understanding of tight gas field development laws.

  7. A novel affinity purification method to isolate peptide specific antibodies

    DEFF Research Database (Denmark)

    Karlsen, Alan E; Lernmark, A; Kofod, Hans

    1990-01-01

    Site-specific, high affinity polyclonal antisera are effectively and successfully produced by immunizing rabbits with synthetic peptides. The use of these antisera in subsequent immune analysis is often limited because of non-specific binding. We describe a new and simple method to effectively...... affinity-purify anti-peptide antibodies. To test our system, rabbits were immunized with model peptides representing sequences of the putative rabbit growth hormone receptor and several HLA-DQ beta-chain molecules. Polystyrene plastic beads were coated with peptides. Immune serum was incubated...... with the beads and after a wash step the bound antibodies were eluted in 1 M acetic acid. The eluted material was composed predominantly of intact immunoglobulin as evidenced by the presence of heavy and light chain bands in SDS-PAGE. The eluted antibodies were peptide specific in ELISA and bound only to intact...

  8. Formal Methods for Abstract Specifications – A Comparison of Concepts

    DEFF Research Database (Denmark)

    Instenberg, Martin; Schneider, Axel; Schnetter, Sabine

    2006-01-01

    In industry formal methods are becoming increasingly important for the verification of hardware and software designs. However current practice for specification of system and protocol functionality on high level of abstraction is textual description. For verification of the system behavior manual...... inspections and tests are usual means. To facilitate the introduction of formal methods in the development process of complex systems and protocols, two different tools evolved from research activities – UPPAAL and SpecEdit – have been investigated and compared regarding their concepts and functionality...

  9. Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity

    Science.gov (United States)

    Tycko, Josh; Myer, Vic E.; Hsu, Patrick D.

    2016-01-01

    Summary Advances in the development of delivery, repair, and specificity strategies for the CRISPR-Cas9 genome engineering toolbox are helping researchers understand gene function with unprecedented precision and sensitivity. CRISPR-Cas9 also holds enormous therapeutic potential for the treatment of genetic disorders by directly correcting disease-causing mutations. Although the Cas9 protein has been shown to bind and cleave DNA at off-target sites, the field of Cas9 specificity is rapidly progressing with marked improvements in guide RNA selection, protein and guide engineering, novel enzymes, and off-target detection methods. We review important challenges and breakthroughs in the field as a comprehensive practical guide to interested users of genome editing technologies, highlighting key tools and strategies for optimizing specificity. The genome editing community should now strive to standardize such methods for measuring and reporting off-target activity, while keeping in mind that the goal for specificity should be continued improvement and vigilance. PMID:27494557

  10. Product Aggregation Bias as a Specification Error in Demand Systems

    OpenAIRE

    George C. Davis

    1997-01-01

    Inherent in all demand studies is some form of product aggregation which can lead to product aggregation bias. This article develops a simple procedure for incorporating product aggregation bias in demand systems that permits testing of product aggregation bias with a standard likelihood ratio test. An empirical illustration of the procedure demonstrates the importance of proper product aggregation. Copyright 1997, Oxford University Press.

  11. Generalized framework for context-specific metabolic model extraction methods

    Directory of Open Access Journals (Sweden)

    Semidán eRobaina Estévez

    2014-09-01

    Full Text Available Genome-scale metabolic models are increasingly applied to investigate the physiology not only of simple prokaryotes, but also eukaryotes, such as plants, characterized with compartmentalized cells of multiple types. While genome-scale models aim at including the entirety of known metabolic reactions, mounting evidence has indicated that only a subset of these reactions is active in a given context, including: developmental stage, cell type, or environment. As a result, several methods have been proposed to reconstruct context-specific models from existing genome-scale models by integrating various types of high-throughput data. Here we present a mathematical framework that puts all existing methods under one umbrella and provides the means to better understand their functioning, highlight similarities and differences, and to help users in selecting a most suitable method for an application.

  12. 14C specific activity of farm products and marine products collected from the Rokkasho area in Aomori prefecture

    International Nuclear Information System (INIS)

    Muranaka, Takeshi; Honda, Kazuya

    1996-01-01

    We investigated 14 C specific activity of farm products and marine products which were collected from the Rokkasho area in Aomori prefecture from 1988 to 1990. The measured 14 C specific activity of farm products was almost equal to one another with the averaged 14 C specific activity of 0.261 [Bq/g·C]. On the other hand, 14 C specific activity of marine products was slightly lower than those of farm products. Especially that of squid was the lowest among studied marine products. This may be due to the low 14 C specific activity of the sea water surrounding squid. (author)

  13. Substrate specificity changes for human reticulocyte and epithelial 15-lipoxygenases reveal allosteric product regulation.

    Science.gov (United States)

    Wecksler, Aaron T; Kenyon, Victor; Deschamps, Joshua D; Holman, Theodore R

    2008-07-15

    Human reticulocyte 15-lipoxygenase (15-hLO-1) and epithelial 15-lipoxygenase (15-hLO-2) have been implicated in a number of human diseases, with differences in their substrate specificity potentially playing a central role. In this paper, we present a novel method for accurately measuring the substrate specificity of the two 15-hLO isozymes and demonstrate that both cholate and specific LO products affect substrate specificity. The linoleic acid (LA) product, 13-hydroperoxyoctadienoic acid (13-HPODE), changes the ( k cat/ K m) (AA)/( k cat/ K m) (LA) ratio more than 5-fold for 15-hLO-1 and 3-fold for 15-hLO-2, while the arachidonic acid (AA) product, 12-( S)-hydroperoxyeicosatetraenoic acid (12-HPETE), affects only the ratio of 15-hLO-1 (more than 5-fold). In addition, the reduced products, 13-( S)-hydroxyoctadecadienoic acid (13-HODE) and 12-( S)-hydroxyeicosatetraenoic acid (12-HETE), also affect substrate specificity, indicating that iron oxidation is not responsible for the change in the ( k cat/ K m) (AA)/( k cat/ K m) (LA) ratio. These results, coupled with the dependence of the 15-hLO-1 k cat/ K m kinetic isotope effect ( (D) k cat/ K m) on the presence of 12-HPETE and 12-HETE, indicate that the allosteric site, previously identified in 15-hLO-1 [Mogul, R., Johansen, E., and Holman, T. R. (1999) Biochemistry 39, 4801-4807], is responsible for the change in substrate specificity. The ability of LO products to regulate substrate specificity may be relevant with respect to cancer progression and warrants further investigation into the role of this product-feedback loop in the cell.

  14. Methods to assess secondary volatile lipid oxidation products in complex food matrices

    DEFF Research Database (Denmark)

    Jacobsen, Charlotte; Yesiltas, Betül

    A range of different methods are available to determine secondary volatile lipid oxidation products. These methods include e.g. spectrophotometric determination of anisidine values and TBARS as well as GC based methods for determination of specific volatile oxidation products such as pentanal...... headspace methods on the same food matrices will be presented....

  15. Risks arising during manipulation with radiologically specific products of biotechnical production

    International Nuclear Information System (INIS)

    Mitrovic, R.; Vukovic, D.; Vicentijevic, M.; Kljajic, R.

    1998-01-01

    Objective of this study was to review the risk of exposure to radiocaesium (134+137Cs), which biologically presents the most toxic radionuclide, of people engaged in the process of supervision, transport and storage of the radiologically specific products, like concentrated protein feedstuff (fish meal, powdered milk), wool, cow hide and chamois. The discussion is based on the results of a radiological-hygienic analysis of these products in the context of veterinary-sanitary control, performed in the authorized referent Laboratory for Radiation Hygiene (LABRAH) at the Scientific Institute for Veterinary Medicine of Serbia in Belgrade (Yugoslavia)

  16. Investigations regarding the lowering of specific intellectual property risks identified in the production process

    Directory of Open Access Journals (Sweden)

    Pakocs Ramona

    2017-01-01

    Full Text Available The main purpose of this research is to decrease the emergence of specific intellectual property risks within the production process as well as increasing risk management performance of IP by preventing them. In order to achieve this, previous studies regarding the main specific intellectual property risks from industrial companies were analyzed together with their managerial methods as well as the possibility of reducing their emergence. As a result of the research conducted were identified five types of intellectual property risks that have a high potential of emergence in the production process, namely: the risk of production of goods in violation of IP rights; the know-how, production knowledge and trade secret disclosure risk; the technological risk of unprotected utility models; the technological risk of unprotected integrated circuits topographies and finally the risk of product counterfeit. In order to achieve the main purpose of our investigation, we have proposed new formulas for estimating the specific intellectual property risks identified in the production process. Their purpose was to minimalize the risk’s negative effects on industrial companies and to increase the managerial performance from the intellectual property domain through a new type of management appropriately named: intellectual property management. The research is finalized with a case study regarding the lapse of rights of a patented invention. Based on a case analysis, it was proved that the exploitation of an invention without a contract represents a counterfeit.

  17. Generating method-specific Reference Ranges - A harmonious outcome?

    Science.gov (United States)

    Lee, Graham R; Griffin, Alison; Halton, Kieran; Fitzgibbon, Maria C

    2017-12-01

    When laboratory Reference Ranges (RR) do not reflect analytical methodology, result interpretation can cause misclassification of patients and inappropriate management. This can be mitigated by determining and implementing method-specific RRs, which was the main objective of this study. Serum was obtained from healthy volunteers (Male + Female, n > 120) attending hospital health-check sessions during June and July 2011. Pseudo-anonymised aliquots were stored (at - 70 °C) prior t° analysis on Abbott ARCHITECT c16000 chemistry and i 2000SR immunoassay analysers. Data were stratified by gender where appropriate. Outliers were excluded statistically (Tukey method) to generate non-parametric RRs (2.5th + 97.5th percentiles). RRs were compared to those quoted by Abbott and UK Pathology Harmony (PH) where possible. For 7 selected tests, RRs were verified using a data mining approach. For chemistry tests (n = 23), Upper or Lower Reference Limits (LRL or URL) were > 20% different from Abbott ranges in 25% of tests (11% from PH ranges) but in 38% for immunoassay tests (n = 13). RRs (mmol/L) for sodium (138-144), potassium (3.8-4.9) and chloride (102-110) were considerably narrower than PH ranges (133-146, 3.5-5.0 and 95-108, respectively). The gender difference for ferritin (M: 29-441, F: 8-193 ng/mL) was more pronounced than reported by Abbott (M: 22-275, F: 5-204 ng/mL). Verification studies showed good agreement for chemistry tests (mean [SD] difference = 0.4% [1.2%]) but less so for immunoassay tests (27% [29%]), particularly for TSH (LRL). Where resource permits, we advocate using method-specific RRs in preference to other sources, particularly where method bias and lack of standardisation limits RR transferability and harmonisation.

  18. 24 CFR 200.955 - Supplementary specific requirements under the HUD building product standard and certification...

    Science.gov (United States)

    2010-04-01

    ... under the HUD building product standard and certification program for fenestration products (windows and... Supplementary specific requirements under the HUD building product standard and certification program for... testing for a product shall be described in the specific building product certification program. In the...

  19. Radioactive ion beam production by the ISOL method for SPIRAL

    International Nuclear Information System (INIS)

    Landre-Pellemoine, Frederique

    2001-01-01

    This work is directly related to the SPIRAL project (Systeme de Production d'Ions Radioactifs Acceleres en Lignes) of which the start up will begin in September 2001 at GANIL (Grand Accelerateur National d'Ions Lourds) in Caen. This thesis primarily concerns the development of radioactive ion production systems (target/ion source) by the thorough study of each production stage of the ISOL (Isotopic Separation On Line) method: target and/or projectile fragmentation production, diffusion out of target material, effusion into the ion source and finally the ionization of the radioactive atoms. A bibliographical research and thermal simulations allowed us to optimize materials and the shape of the production and diffusion targets. A first target was optimized and made reliable for the radioactive noble gases production (argon, neon...). A second target dedicated to the radioactive helium production was entirely designed and realised (from the specifications to the 'off line' and 'on line' tests). Finally, a third target source system was defined for singly-charged radioactive alkaline production. The intensities of secondary beams planned for SPIRAL are presented here. A detailed study of the diffusion effusion efficiency for these various targets showed that the use of a fine microstructure carbon (grain size of 1 μm) improved the diffusion and showed the importance of thickness of the lamella for the short lived isotope effusion. (author) [fr

  20. Introducing Products to DoD Using Specifications and Standards

    Science.gov (United States)

    2011-08-18

    to utilize the Product Introduction Tool. Search ~Favorites .S » Links ~Customize Links ~ EDS-NMCI ~Free Hotmail Product Introduction Process User...the Product Introduction Tool. Search ~Favorites .S » Links ~Customize Links ~ EDS-NMCI ~Free Hotmail Product Introduction Process User Pol icy...Links i1 EDS-NMCI ~ Free Hotmail i] I] Go ldentitify Categories/Subcategories Identify the category/subcategory that most closely covers your

  1. Periodic local MP2 method employing orbital specific virtuals

    International Nuclear Information System (INIS)

    Usvyat, Denis; Schütz, Martin; Maschio, Lorenzo

    2015-01-01

    We introduce orbital specific virtuals (OSVs) to represent the truncated pair-specific virtual space in periodic local Møller-Plesset perturbation theory of second order (LMP2). The OSVs are constructed by diagonalization of the LMP2 amplitude matrices which correspond to diagonal Wannier-function (WF) pairs. Only a subset of these OSVs is adopted for the subsequent OSV-LMP2 calculation, namely, those with largest contribution to the diagonal pair correlation energy and with the accumulated value of these contributions reaching a certain accuracy. The virtual space for a general (non diagonal) pair is spanned by the union of the two OSV sets related to the individual WFs of the pair. In the periodic LMP2 method, the diagonal LMP2 amplitude matrices needed for the construction of the OSVs are calculated in the basis of projected atomic orbitals (PAOs), employing very large PAO domains. It turns out that the OSVs are excellent to describe short range correlation, yet less appropriate for long range van der Waals correlation. In order to compensate for this bias towards short range correlation, we augment the virtual space spanned by the OSVs by the most diffuse PAOs of the corresponding minimal PAO domain. The Fock and overlap matrices in OSV basis are constructed in the reciprocal space. The 4-index electron repulsion integrals are calculated by local density fitting and, for distant pairs, via multipole approximation. New procedures for determining the fit-domains and the distant-pair lists, leading to higher efficiency in the 4-index integral evaluation, have been implemented. Generally, and in contrast to our previous PAO based periodic LMP2 method, the OSV-LMP2 method does not require anymore great care in the specification of the individual domains (to get a balanced description when calculating energy differences) and is in that sense a black box procedure. Discontinuities in potential energy surfaces, which may occur for PAO-based calculations if one is not

  2. Periodic local MP2 method employing orbital specific virtuals

    Energy Technology Data Exchange (ETDEWEB)

    Usvyat, Denis, E-mail: denis.usvyat@chemie.uni-regensburg.de; Schütz, Martin, E-mail: martin.schuetz@chemie.uni-regensburg.de [Institute for Physical and Theoretical Chemistry, Universität Regensburg, Universitätsstraße 31, D-93040 Regensburg (Germany); Maschio, Lorenzo, E-mail: lorenzo.maschio@unito.it [Dipartimento di Chimica, and Centre of Excellence NIS (Nanostructured Interfaces and Surfaces), Università di Torino, via Giuria 5, I-10125 Torino (Italy)

    2015-09-14

    We introduce orbital specific virtuals (OSVs) to represent the truncated pair-specific virtual space in periodic local Møller-Plesset perturbation theory of second order (LMP2). The OSVs are constructed by diagonalization of the LMP2 amplitude matrices which correspond to diagonal Wannier-function (WF) pairs. Only a subset of these OSVs is adopted for the subsequent OSV-LMP2 calculation, namely, those with largest contribution to the diagonal pair correlation energy and with the accumulated value of these contributions reaching a certain accuracy. The virtual space for a general (non diagonal) pair is spanned by the union of the two OSV sets related to the individual WFs of the pair. In the periodic LMP2 method, the diagonal LMP2 amplitude matrices needed for the construction of the OSVs are calculated in the basis of projected atomic orbitals (PAOs), employing very large PAO domains. It turns out that the OSVs are excellent to describe short range correlation, yet less appropriate for long range van der Waals correlation. In order to compensate for this bias towards short range correlation, we augment the virtual space spanned by the OSVs by the most diffuse PAOs of the corresponding minimal PAO domain. The Fock and overlap matrices in OSV basis are constructed in the reciprocal space. The 4-index electron repulsion integrals are calculated by local density fitting and, for distant pairs, via multipole approximation. New procedures for determining the fit-domains and the distant-pair lists, leading to higher efficiency in the 4-index integral evaluation, have been implemented. Generally, and in contrast to our previous PAO based periodic LMP2 method, the OSV-LMP2 method does not require anymore great care in the specification of the individual domains (to get a balanced description when calculating energy differences) and is in that sense a black box procedure. Discontinuities in potential energy surfaces, which may occur for PAO-based calculations if one is not

  3. Method for alignment of product and production concepts

    DEFF Research Database (Denmark)

    Pedersen, Rasmus; Kvist, Morten; Mortensen, Niels Henrik

    2005-01-01

    The right use of modular product architectures can help companies provide a great variety of customized products at a competitive price level, by reuse of knowledge, components, processes and utilization of economies of scale in many of the activities that are necessary to provide products...... for customers. Modular product architectures often serve as a basis for several products often referred to as product families. One or more product families may constitute a substantial amount of the total product portfolio of a company, and it is, therefore, of the greatest importance that concepts...... and solutions are evaluated thoroughly throughout the design phases because any design fault is likely to propagate to a large part of the business. Developing modular product architectures is therefore also the task of ensuring a fit between the products that can be made on the basis of the architecture...

  4. Method for the production of a hydrophilic polymer product

    International Nuclear Information System (INIS)

    Cordrey, P.W.; Frankland, J.D.; Highgate, D.J.

    1976-01-01

    It has been found that by subjecting mixtures containing hydrophilic monomer materials to radiation it is possible to obtain polymers capable of absorbing up to five times or more their weight of water. These polymers are very suitable for use in contact with living tissue since they contain none of the harmful contaminants derived from initiators used in conventional polymerisation. A method for the production of these polymers comprises subjecting to irradiation polymerisation a mixture containing (1) at least one hydrophilic monomer selected from N-vinyl pyrrolidones and hydroxyalkyl methacrylates and at least one hydrophobic monomer selected from alkyl acrylates, alkyl methacrylates and styrene, or (2) at least one hydrophilic monomer such as N-vinyl-2-pyrrolidone or hydroxyethyl methacrylate and at least one hydrophobic polymer selected from nylons, polyamides and terephthalic acid, with one or more alkyl substituted hexamethylene diamines, polyalkyl acrylates, polyalkyl methacrylates, polystyrenes, polyvinyl chloride and bisphenol polycarbonate. The irradiation may be gamma-ray, and the dosage 2 to 5 MR over one to 48 hours. The polymerisation may be carried out in the presence of a cross-linking agent such as alkyl methacrylate, divinylbenzene, or ethylene glycol dimethacrylate and in an oxygen-free or inert atmosphere, or in vacuo. Examples of application of the method are given. (U.K.)

  5. Progress in the methods of steel production

    Energy Technology Data Exchange (ETDEWEB)

    Onodera, S; Suzuki, K

    1979-07-01

    The steel making and ingot making processes have made a great progress in the past two decades, which has been one of the direct supports for developing those megalo-technologies as in iron and steel making, petrochemical, fossil and nuclear power generation, and other industries. In discussing steel quality, large or small, everything is to the ingot, and in order to reduce miscellaneous imperfections in steel ingots, for largest ones in particular, tremendous efforts have been made so far. In addition to the improvements in conventional ingot making method, a number of innovational techniques were developed such as ESR, PESC, ABR, MHKW, BEST, etc. with some results in respective fields of application. Nevertheless, for largest sizes of ingot from 250 to 500 tons, the conventional method was successful enough. The history of conventional method is summarized for the past quarter century. The improved quality of large ingots could bring some more merits in the final forged products, which are: (1) improved isotropy of mechanial properties; and (2) sufficnet effect of forging with much less forging ratio.

  6. Methodical Specifics of Thermal Experiments with Thin Carbon Reinforced Plates

    Directory of Open Access Journals (Sweden)

    O. V. Denisov

    2015-01-01

    Full Text Available Polymer composite materials (CM are widely used in creation of large space constructions, especially reflectors of space antennas. Composite materials should provide high level of specific stiffness and strength for space structures. Thermal conductivity in reinforcement plane is a significant factor in case of irregular heating space antennas. Nowadays, data on CM reinforcement plane thermal conductivity are limited and existing methods of its defining are imperfect. Basically, traditional methods allow us to define thermal conductivity in perpendicular direction towards the reinforcement plane on the samples of round or rectangular plate. In addition, the thickness of standard samples is larger than space antenna thickness. Consequently, new methods are required. Method of contact heating, which was developed by BMSTU specialists with long hollow carbon beam, could be a perspective way. This article is devoted to the experimental method of contact heating on the thin carbon plates.Thermal tests were supposed to provide a non-stationary temperature field with a gradient being co-directional with the plane reinforcement in the material sample. Experiments were conducted in vacuum chamber to prevent unstructured convection. Experimental thermo-grams processing were calculated by 1-d thermal model for a thin plate. Influence of uncertainty of experimental parameters, such as (radiation emission coefficients of sample surface, glue, temperature sensors and uncertainty of sensors placement on the result of defined thermal conductivity has been estimated. New data on the thermal conductivity in reinforcement plane were obtained within 295 - 375 K temperature range, which can be used to design and develop reflectors of precision space antennas. In the future it is expedient to conduct tests of thin-wall plates from carbon fiber-reinforced plastic in wide temperature range, especially in the low-range temperatures.

  7. Specific binding-adsorbent assay method and test means

    International Nuclear Information System (INIS)

    1981-01-01

    A description is given of an improved specific binding assay method and test means employing a nonspecific adsorbent for the substance to be determined, particularly hepatitis B surface (HBsub(s)) antigen, in its free state or additionally in the form of its immune complex. The invention is illustrated by 1) the radioimmunoadsorbent assay for HBsub(s) antigen, 2) the radioimmunoadsorbent assay for HBsub(s) antigen in the form of immune complex with antibody, 3) a study of adsorption characteristics of various anion exchange materials for HBsub(s) antigen, 4) the use of hydrophobic adsorbents in a radioimmunoadsorbent assay for HBsub(s) antigen and 5) the radioimmunoadsorbent assay for antibody to HBsub(s) antigen. The advantages of the present method for detecting HBsub(s) antigen compared to previous methods include the manufacturing advantages of eliminating the need for insolubilised anti-HBsub(s) and the advantages of a single incubation step, fewer manipulations, storability of adsorbent materials, increased sensitivity and versatility of detecting HBsub(s) antigen in the form of its immune complex if desired. (U.K.)

  8. Using budget-friendly methods to analyze sport specific movements

    Science.gov (United States)

    Jackson, Lindsay; Williams, Sarah; Ferrara, Davon

    2015-03-01

    When breaking down the physics behind sport specific movements, athletes, usually professional, are often assessed in multimillion-dollar laboratories and facilities. Budget-friendly methods, such as video analysis using low-cost cameras, iPhone sensors, or inexpensive force sensors can make this process more accessible to amateur athletes, which in-turn can give insight into injury mechanisms. Here we present a comparison of two methods of determining the forces experienced by a cheerleader during co-education stunting and soccer goalies while side-diving. For the cheerleader, accelerometer measurements were taken by an iPhone 5 and compared to video analysis. The measurements done on the soccer players were taken using FlexiForce force sensors and again compared to video analysis. While these budget-friendly methods could use some refining, they show promise for producing usable measurements for possibly increasing our understanding of injury in amateur players. Furthermore, low-cost physics experiments with sports can foster an active learning environment for students with minimum physics and mathematical background.

  9. Nuclear fuel particle and method of production

    International Nuclear Information System (INIS)

    Wagner-Loffler, M.

    1975-01-01

    The core consisting of fuel oxide (UO 2 or Th or Pu oxide) of a fuel particle coated with carbon-contained material is enriched with a small addition (max 6 wt.%) of a Ba or Sr compound (atomic ratio for nuclear fuel oxide Ba being 5 - 10 : 1) which is to prevent fission products breaking the protective carbon and/or silicon carbide coating; the Ba or Sr molybdate generated is to reduce the pressure of the carbon dioxide produced. Methods to manufacture such nuclear fuel particles are proposed where 1) an agglomerisation and shaping of the spheres in a fast cycling bowle and 2) a formation of drops from a colloidal solution which are made to congeal in a liquid paraffin column, take place followed by the pyrolytic coating of the particles. (UWI/LH) [de

  10. Simulation methods for nuclear production scheduling

    International Nuclear Information System (INIS)

    Miles, W.T.; Markel, L.C.

    1975-01-01

    Recent developments and applications of simulation methods for use in nuclear production scheduling and fuel management are reviewed. The unique characteristics of the nuclear fuel cycle as they relate to the overall optimization of a mixed nuclear-fossil system in both the short-and mid-range time frame are described. Emphasis is placed on the various formulations and approaches to the mid-range planning problem, whose objective is the determination of an optimal (least cost) system operation strategy over a multi-year planning horizon. The decomposition of the mid-range problem into power system simulation, reactor core simulation and nuclear fuel management optimization, and system integration models is discussed. Present utility practices, requirements, and research trends are described. 37 references

  11. Method for the enzymatic production of hydrogen

    Science.gov (United States)

    Woodward, J.; Mattingly, S.M.

    1999-08-24

    The present invention is an enzymatic method for producing hydrogen comprising the steps of: (a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing oxidation within a catabolic reaction, such as glucose, galactose, xylose, mannose, sucrose, lactose, cellulose, xylan and starch; the reaction mixture also comprising an amount of glucose dehydrogenase in an amount sufficient to catalyze the oxidation of the substrate, an amount of hydrogenase sufficient to catalyze an electron-requiring reaction wherein a stoichiometric yield of hydrogen is produced, an amount of pH buffer in an amount sufficient to provide an environment that allows the hydrogenase and the glucose dehydrogenase to retain sufficient activity for the production of hydrogen to occur and also comprising an amount of nicotinamide adenine dinucleotide phosphate sufficient to transfer electrons from the catabolic reaction to the electron-requiring reaction; (b) heating the reaction mixture at a temperature sufficient for glucose dehydrogenase and the hydrogenase to retain sufficient activity and sufficient for the production of hydrogen to occur, and heating for a period of time that continues until the hydrogen is no longer produced by the reaction mixture, wherein the catabolic reaction and the electron-requiring reactions have rates of reaction dependent upon the temperature; and (c) detecting the hydrogen produced from the reaction mixture. 8 figs.

  12. Production capability: ERDA methods and results

    International Nuclear Information System (INIS)

    Klemenic, J.

    1977-01-01

    Production centers are categorized into four classes, according to the relative certainty of future production. A ''forward cost'' basis is used to establish both the resource base and to define the acceptable production centers. The first phase of the work is called the ''Could'' capability. Resources are assigned to existing production centers, or new production centers are postulated based on adequate resources to support a mill for a reasonable economic life. A production schedule is developed for each center. The last step in the ''Could'' study is to aggregate the capital and operating costs. The final step in the Production Capability study is the rescheduling of the production from the ''Could'' to produce only sufficient U concentrate to meet the feed requirements of enrichment facilities operated at the announced transaction tails assay plans. The optimized production schedules are called the ''Need'' production capability. A separate study was also performed of industry production plans. 4 tables, 7 figs

  13. Specificity of Good Manufacturing Practice (GMP) for Biomedical Cell Products.

    Science.gov (United States)

    Tulina, M A; Pyatigorskaya, N V

    2018-03-01

    The article describes special aspects of Good Manufacturing Practice (GMP) for biomedical cell products (BMCP) that imply high standards of aseptics throughout the entire productio process, strict requirements to donors and to the procedure of biomaterial isolation, guaranty of tracing BMCP products, defining processing procedures which allow to identify BMCP as minimally manipulated; continuous quality control and automation of the control process at all stages of manufacturing, which will ensure product release simultaneously with completion of technological operations.

  14. The determination and analysis of site-specific rates of mitochondrial reactive oxygen species production

    DEFF Research Database (Denmark)

    Quinlan, Casey L; Perevoschikova, Irina V; Goncalves, Renata L S

    2013-01-01

    Mitochondrial reactive oxygen species (ROS) are widely implicated in physiological and pathological pathways. We propose that it is critical to understand the specific sites of mitochondrial ROS production and their mechanisms of action. Mitochondria possess at least eight distinct sites of ROS...... production in the electron transport chain and matrix compartment. In this chapter, we describe the nature of the mitochondrial ROS-producing machinery and the relative capacities of each site. We provide detailed methods for the measurement of H2O2 release and the conditions under which maximal rates from...

  15. Methods for Attributing Land-Use Emissions to Products

    Science.gov (United States)

    Davis, S. J.; Burney, J. A.; Pongratz, J.; Caldeira, K.

    2014-12-01

    Roughly one-third of anthropogenic GHG emissions are caused by agricultural and forestry activities and land-use change (collectively, 'land-use emissions'). Understanding the ultimate drivers of these emissions requires attributing emissions to specific land-use activities and products. Although quantities of land-use emissions are matters of fact, the methodological choices and assumptions required to attribute those emissions to activities and products depend on research goals and data availability. We will demonstrate several possible accounting methods, highlighting the sensitivity of accounting to temporal distributions of emissions and the consequences of replacing spatially-explicit data with aggregate proxies such as production or harvested area data. Different accounting options emphasize different causes of land-use emissions (e.g., proximate or indirect drivers of deforestation). To support public policies that effectively balance competing objectives, analysts should carefully consider and communicate implications of accounting choices.

  16. 75 FR 33311 - Guidance for Industry on Bioequivalence Recommendations for Specific Products; Availability

    Science.gov (United States)

    2010-06-11

    ... available recommendations on how to design product-specific BE studies to support ANDAs. Under this process... thinking on a new process for making available to sponsors FDA guidance on how to design product-specific... ``Bioequivalence Recommendations for Specific Products.'' This guidance describes a new process for making...

  17. 77 FR 10535 - Final Guidances for Industry Describing Product-Specific Bioequivalence Recommendations...

    Science.gov (United States)

    2012-02-22

    ... recommendations provide product-specific guidance on the design of BE studies to support abbreviated new drug... the process that would be used to make product-specific BE recommendations available to the public on... ``Bioequivalence Recommendations for Specific Products,'' which explained the process that would be used to make...

  18. Methods of reducing non-specific adsorption in microfluidic biosensors

    International Nuclear Information System (INIS)

    Choi, Seokheun; Chae, Junseok

    2010-01-01

    Non-specific adsorption (NSA) of biomolecules is a persistent challenge in microfluidic biosensors. Microfluidic biosensors often have immobilized bioreceptors such as antibodies, enzymes, DNAs, etc, via linker molecules such as SAMs (self-assembled monolayers) to enhance immobilization. However, the linker molecules are very susceptible to NSA, causing false responses and decreasing sensitivity. In this paper, we present design methods to reduce the NSA of alkanethiol SAMs, which are popular linker molecules on microfluidic biosensors. Three design parameters were studied for two different chain-length SAMs (n = 2 and 10): (i) SAM incubation time, (ii) surface roughness [0.8 nm and 4.4 nm RMS (root mean square)] and (iii) gold crystal re-growth along (1 1 1) the target orientation. NSA was monitored by surface plasmon resonance (SPR). The results suggest that increased SAM incubation time reduces NSA, and that short-chain SAMs respond more favorably than the long-chain SAMs. Both SAMs were shown to be sensitive to surface roughness, and long-chain SAMs reduced NSA by 75%. Gold crystal re-growth along (1 1 1) the target orientation profoundly reduced NSA on the short-chain SAM. On a gold surface where surface roughness was 0.8 nm and there was strong directional alignment along the (1 1 1) gold crystal, final concentrations of nonspecifically bound proteins were 0.05 ng mm −2 (fibrinogen) and 0.075 ng mm −2 (lysozyme)—significantly lower than other known methods. The results show that optimizing three parameters (SAM incubation time, gold surface roughness and gold crystal orientation) improved SAM sensitivity for fibrinogen–anti-fibrinogen conjugates by a factor of 5 in 2.94 pM, suggesting that the methods are effective for reducing NSA in microfluidic biosensors.

  19. Electrospinning versus fibre production methods: from specifics to technological convergence

    NARCIS (Netherlands)

    Luo, C.J.; Stoyanov, S.D.; Stride, E.; Pelan, E.; Edirisinghe, M.

    2012-01-01

    Academic and industrial research on nanofibres is an area of increasing global interest, as seen in the continuously multiplying number of research papers and patents and the broadening range of chemical, medical, electrical and environmental applications. This in turn expands the size of the market

  20. Method for creating and visualizing product architectures

    DEFF Research Database (Denmark)

    Kvist, Morten; Pedersen, Rasmus; Mortensen, Niels Henrik

    2005-01-01

    Over the past few decades the notion of product architectures in development has obtained increasing interest from many corporations conducting product development. Modular architectures and product platforms are often reported to have great potential to reduce internal complexity and total cost ...

  1. Method of manufacturing borosilicate glass solidification products

    International Nuclear Information System (INIS)

    Tanaka, Tsuneya.

    1986-01-01

    Purpose: To obtain glass solidification products efficiently in a dry process from medium and high level radioactive liquid wastes discharged from PWR type reactors. Method: Boric acid-containing radioactive liquid wastes generated from primary coolants of PWR type reactors are evaporated to condensate as the pre-treatment. The concentrated liquid wastes are supplied to a drum type rotary kiln. While on the other hand, usual glass frits are introduced into the kiln. The liquid wastes are dried in the rotary kiln, as well as B 2 O 3 and the glass frits in the liquid wastes are combined into glass particles. In this case, since the kiln is rotated, no glass particles are deposited on the wall of the kiln. Then, the glass particles are introduced for melting into a high frequency melting furnace made of metal. The melting temperature is set to 1100 - 1150 deg C. The molten borosilicate glass is recovered from the bottom of the melting furance, contained in a canister and cooled for several hours, and then a cover is welded to the canister. (Ikeda, J.)

  2. Tobacco Product Use Patterns, and Nicotine and Tobacco-Specific Nitrosamine Exposure: NHANES 1999-2012.

    Science.gov (United States)

    Choi, Kelvin; Sabado, Melanie; El-Toukhy, Sherine; Vogtmann, Emily; Freedman, Neal D; Hatsukami, Dorothy

    2017-10-01

    Background: Few studies have examined differences in product consumption patterns and nicotine and tobacco-specific nitrosamines (TSNA) exposure between single versus dual- and poly-tobacco users. We applied the Tobacco Product Use Patterns (T-PUPs) model to fill this gap in the literature. Methods: Data from adults (age ≥18 years) who used any tobacco products during the 5 days prior to participating in the 1999-2012 National Health and Nutrition Examination Survey (NHANES) were analyzed. Participants were classified into seven T-PUPs: (1) cigarettes only, (2) noncigarette combustibles only, (3) noncombustibles only, (4) dual noncigarette combustibles and noncombustibles, (5) dual cigarettes and noncombustibles, (6) dual cigarettes and noncigarette combustibles, and (7) poly-tobacco use. Weighted regression models were used to compare product consumption, serum cotinine, and urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (i.e., NNAL) levels between single-, dual-, and poly-tobacco T-PUPs. Results: Dual- and poly-tobacco T-PUPs were associated with lower product consumption compared with single-product T-PUPs only in some cases (e.g., dual cigarette and noncombustible users smoked cigarettes on 0.6 fewer days in the past 5 days compared with cigarette-only users; P product T-PUPs. Conclusions: Product consumption, and nicotine and TSNAs exposure of dual- and poly-tobacco product category users somewhat differ from those of single-product category users as defined by the T-TUPs model. Impact: Higher levels of cotinine and NNAL among dual- and poly-tobacco T-TUPs users compared with the single-product T-TUPs users may indicate health concerns. Cancer Epidemiol Biomarkers Prev; 26(10); 1525-30. ©2017 AACR . ©2017 American Association for Cancer Research.

  3. Quality estimation methods used in product life cycle

    OpenAIRE

    M. Dudek-Burlikowska; D. Szewieczek

    2007-01-01

    Purpose: A new approach to quality control in production company with usage of quality research methods has been presented.Design/methodology/approach: The possibility of usage of quality research methods are connected with continuous quality improvement of pre-production, production and after-production spheres of organization. Interdependence of the quality research methods and product life cycle has been taken into account.Findings: At the present time the enterprises should integrate qua...

  4. Testing of toxicity based methods to develop site specific clean up objectives - phase 1: Toxicity protocol screening and applicability

    International Nuclear Information System (INIS)

    Hamilton, H.; Kerr, D.; Thorne, W.; Taylor, B.; Zadnik, M.; Goudey, S.; Birkholz, D.

    1994-03-01

    A study was conducted to develop a cost-effective and practical protocol for using bio-assay based toxicity assessment methods for remediation of decommissioned oil and gas production, and processing facilities. The objective was to generate site-specific remediation criteria for contaminated sites. Most companies have used the chemical-specific approach which, however, did not meet the ultimate land use goal of agricultural production. The toxicity assessment method described in this study dealt with potential impairment to agricultural crop production and natural ecosystems. Human health concerns were not specifically addressed. It was suggested that chemical-specific methods should be used when human health concerns exist. . Results showed that toxicity tests will more directly identify ecological stress caused by site contamination than chemical-specific remediation criteria, which can be unnecessarily protective. 11 refs., 7 tabs., 6 figs

  5. New methods of milk products preservation

    International Nuclear Information System (INIS)

    Baca, E.; Skarzynska, M.

    1995-01-01

    There are discussed the following methods: microwave heating; Ohmic heating; use of infrared, ultraviolet and gamma irradiation; heating by extrusion, heating by high pressure homogenization and combined method

  6. Specificities of micro-reactors for hydrogen production and purification

    Energy Technology Data Exchange (ETDEWEB)

    Mirodatos, C.; Dupont, N.; Germani, G.; Veen, A. C. ven; Schuurman, Y.

    2005-07-01

    Sustainable chemistry and exploitation of energy sources for the next decades requires considerable progress in process intensification. A development of new tools and equipments meeting the objectives of high efficiency, improved safety, compactness and low implementation costs is therefore subject of intensive research effort. Among the various scenarios tested in R and D, micro-structured reactors appear as a highly promising technology 1 and perspectives of mass production are already announced by technology providers 2. These reactors are based on assembly/stacking of micro structured plates or fibres. Due to their high heat and/or mass transfer, low pressure drop and good phase contacting, they sound particularly adapted to the large domain of hydrogen production by fuel reforming and purification. This presentation aims at outlining the state of the art, the advantages and drawbacks of using micro-structured reactors to intensify hydrogen production and purification. Two case studies will illustrate this approach: i) comparison between fixed bed and micro-structured reactor for the reforming of methanol into hydrogen and carbon oxides and ii) use of those devices in kinetic studies on the WGS reaction. (Author)

  7. 24 CFR 200.940 - Supplementary specific requirements under the HUD building product standards and certification...

    Science.gov (United States)

    2010-04-01

    ... under the HUD building product standards and certification program for sealed insulating glass units... Supplementary specific requirements under the HUD building product standards and certification program for... specific building product certification program. In the case of sealed insulating glass units, testing and...

  8. New Synthetic Methods for Hypericum Natural Products

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, Insik [Iowa State Univ., Ames, IA (United States)

    2006-01-01

    Organic chemistry has served as a solid foundation for interdisciplinary research areas, such as molecular biology and medicinal chemistry. An understanding of the biological activities and structural elucidations of natural products can lead to the development of clinically valuable therapeutic options. The advancements of modern synthetic methodologies allow for more elaborate and concise natural product syntheses. The theme of this study centers on the synthesis of natural products with particularly challenging structures and interesting biological activities. The synthetic expertise developed here will be applicable to analog syntheses and to other research problems.

  9. Express method for determining productivity of deposits

    Energy Technology Data Exchange (ETDEWEB)

    Bobrovnik, M D; Gurzhiy, D V

    1980-01-01

    It is indicated that the practice of studying cross sectional views in the Ciscarpathian oil and gas province demonstrated the existence of a direct link between productivity of deposits and the degree of transformation of bitumens in rock. The bituminous matter which fills the micropores and microfractures in rocks in the productive part of the section have light tones of brown color, in the transcontour part of the field it is dark brown and black. In the water-bearing part of the section, oxidized bitumens are noted in the rock jointly with iron hydroxides. This law can be used to correct the conclusions, data on field geophysics, productivity of deposits.

  10. Production of N-13 labeled compounds with high specific activity

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Kazutoshi; Sasaki, Motoji; Yoshida, Yuichiro; Haradahira, Terushi; Inoue, Osamu [National Inst. of Radiological Sciences, Chiba (Japan)

    1997-03-01

    Nitrogen-13 was produced by irradiating ultra pure water saturated with a pure gas (N2, O2, He, H2) with 18 MeV protons. Ion species generated by irradiation were analyzed with radio ion chromatography systems. An automated equipment was developed to synthesize anhydrous (13N)NH3 as a synthetic precursor and (13N)p-nitrophenyl carbamate ((13N)NPC) as a model compound, using the (13N)NH3. The radiochemical yield and specific activity of (13N)NPC was high enough to carry out the receptor study with PET. (author)

  11. Sex-specific vitellogenin production in immature rainbow trout

    Energy Technology Data Exchange (ETDEWEB)

    Carlson, D.B.; Williams, D.E.

    1999-10-01

    Many xenobiotics interact with hormone systems of animals, potentially leading to a phenomenon commonly called endocrine disruption. Much attention has focused on steroid hormone systems and corresponding receptor proteins, particularly estrogens. Vitellogenin (Vg) was measured in sexually immature rainbow trout (Oncorhynchus mykiss) exposed to 17{beta}-estradiol (E{sub 2}) in the diet. Mixed-sex populations of trout aged 3, 6, 12, or 18 months were maintained separately and fed E{sub 2} at 0.05 or 2.5 mg/kg for 7d. Females fed E{sub 2} at 0.05 mg/kg consistently produced three- to fourfold greater amounts of Vg than similarly aged males. Age- and sex-matched fish fed E{sub 2} at 2.5 mg/kg produced equivalent amounts of Vg. Sex differences in Vg production were apparent only at a dose of E{sub 2} (0.05 mg/kg) that results in submaximal Vg induction. Their results document the importance of considering the sex of juvenile fish when using Vg production as a marker of xenoestrogen exposure.

  12. Compositions and methods for xylem-specific expression in plant cells

    Energy Technology Data Exchange (ETDEWEB)

    Han, Kyung-Hwan; Ko, Jae-Heung

    2017-12-19

    The invention provides promoter sequences that regulate specific expression of operably linked sequences in developing xylem cells and/or in developing xylem tissue. The developing xylem-specific sequences are exemplified by the DX5, DX8, DX11, and DX15 promoters, portions thereof, and homologs thereof. The invention further provides expression vectors, cells, tissues and plants that contain the invention's sequences. The compositions of the invention and methods of using them are useful in, for example, improving the quantity (biomass) and/or the quality (wood density, lignin content, sugar content etc.) of expressed biomass feedstock products that may be used for bioenergy, biorefinary, and generating wood products such as pulp, paper, and solid wood.

  13. Production of carboxy-terminal specific antiserum against glucagon

    International Nuclear Information System (INIS)

    Liu Yibing; Han Shiquan

    1993-01-01

    To produce carboxy-terminal specific antisera against glucagon was coupled mainly via its amino terminal histidine to thyroglobulin, using the amino group reactive pentandiol at pH 7.0 for the conjugation procedure. After repeated immunization of guinea pigs and rabbits, the antisera were obtained. The titer of guinea pig antiserum against glucagon was 1:3000-1:35000 and affinity constant was 9.3 x 10 10 -11.4 x 10 10 l · mol -1 . There were no cross reaction with GIP, INS, Copeptide and gastrin. The titer of rabbit antiserum against glucagon was 1:900-1:9000 and affinity constant was 0.36 x 10 10 -3.9 x 10 10 l · mol -1 . There were no cross reaction with INS, C-peptide and gastrin. The cross reaction with GIP was 0.02%

  14. Production of carboxy-terminal specific antiserum against glucagon

    Energy Technology Data Exchange (ETDEWEB)

    Yibing, Liu; Shiquan, Han [Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy

    1993-02-01

    To produce carboxy-terminal specific antisera against glucagon was coupled mainly via its amino terminal histidine to thyroglobulin, using the amino group reactive pentandiol at pH 7.0 for the conjugation procedure. After repeated immunization of guinea pigs and rabbits, the antisera were obtained. The titer of guinea pig antiserum against glucagon was 1:3000-1:35000 and affinity constant was 9.3 x 10[sup 10]-11.4 x 10[sup 10] l [center dot] mol[sup -1]. There were no cross reaction with GIP, INS, Copeptide and gastrin. The titer of rabbit antiserum against glucagon was 1:900-1:9000 and affinity constant was 0.36 x 10[sup 10]-3.9 x 10[sup 10] l [center dot] mol[sup -1]. There were no cross reaction with INS, C-peptide and gastrin. The cross reaction with GIP was 0.02%.

  15. Radiation methods in dairy production and processing

    International Nuclear Information System (INIS)

    Ganguli, N.C.

    1975-01-01

    Various uses of radiotracers and radiation in dairy technology are described. In dairy production, radiotracers are used for studying: (1) rumen metabolism leading to protein synthesis (2) total body water, blood volume and sodium (3) minerals metabolism (4) relation between climatic stress and thyroid functioning of dairy animals (5) volume of milk in mammary glands (6) hormone level in dairy animals and (7) spermatozoa metabolism. In dairy processing, radiotracers are used for studying: (1) compositional analysis of milk and milk products and (2) efficiency of cleaning agents for cleaning dairy equipment. Ionizing radiation is used for: (1) preservation of milk and milk products and (2) sterilization of packaging materials. Radiation source has been used to monitor the over-run in ice-cream and the fill control for fluid in papar cartons. (M.G.B.)

  16. Chapter 21: Estimating Net Savings - Common Practices. The Uniform Methods Project: Methods for Determining Energy Efficiency Savings for Specific Measures

    Energy Technology Data Exchange (ETDEWEB)

    Kurnik, Charles W [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Violette, Daniel M. [Navigant, Boulder, CO (United States); Rathbun, Pamela [Tetra Tech, Madison, WI (United States)

    2017-11-02

    This chapter focuses on the methods used to estimate net energy savings in evaluation, measurement, and verification (EM and V) studies for energy efficiency (EE) programs. The chapter provides a definition of net savings, which remains an unsettled topic both within the EE evaluation community and across the broader public policy evaluation community, particularly in the context of attribution of savings to a program. The chapter differs from the measure-specific Uniform Methods Project (UMP) chapters in both its approach and work product. Unlike other UMP resources that provide recommended protocols for determining gross energy savings, this chapter describes and compares the current industry practices for determining net energy savings but does not prescribe methods.

  17. Dual substrate feedback control of specific growth-rate in vaccine production

    NARCIS (Netherlands)

    Neeleman, R.; Beuvery, E.C.; Vries, D.; Straten, van G.; Boxtel, van A.J.B.

    2004-01-01

    Abstract: Unexpectedly, primary concern of bio-pharmaceutical industry is not optimisation of product yield or cost reduction, but consistency in production and product quality. This paper describes the methodology and experimental results of specific growth-rate control for vaccine production. The

  18. Identified adjustability dimensions when generating a product specific requirements specification by requirements reuse

    DEFF Research Database (Denmark)

    Hauksdóttir, Dagný; Mortensen, Niels Henrik; Nielsen, Poul Erik

    2014-01-01

    . An extensive state of the art is included to introduce the presented methods related to each adjustability dimensions. The options for implementing each adjustability dimensions in a requirement reuse approach are illustrated along with a discussion regarding the benefits and issues resulting from each option....... This discussion should help practitioners to better understand the possible methods that can be implemented and to design a user friendly and sustainable approach. A case study, describing how the dimensions are incorporated in two requirements reuse approaches, for Danfoss Solar Inverters (SI) and Danfoss...

  19. Method and apparatus for furfural production

    OpenAIRE

    Marcotullio, G.; De Jong, W.

    2015-01-01

    The invention concerns a process for the production of furfural from pentoses and/or water soluble pentosans, comprising the steps of feeding an aqueous pentose solution containing one or more pentoses or soluble pentosans to the bottom of a reaction column to produce an aqueous liquid upflow; heating the bottom part of the column using a heat exchanger to produce a steam upflow in the column; recovering a vapour product stream containing water and furfural from the top of the column; and con...

  20. Production of specific-structured lipids by enzymatic interesterification in a pilot continuous enzyme bed reactor

    DEFF Research Database (Denmark)

    Xu, Xuebing; Balchen, Steen; Høy, Carl-Erik

    1998-01-01

    Production of specific-structured lipids (interesterified lipids with a specific structure) by enzymatic interesterification was carried out in a continuous enzyme bed pilot scale reactor. Commercial immobilized lipase (Lipozyme IM) was used and investigations of acyl migration, pressure drop...

  1. Histogram specification as a method of density modification

    International Nuclear Information System (INIS)

    Harrison, R.W.

    1988-01-01

    A new method for improving the quality and extending the resolution of Fourier maps is described. The method is based on a histogram analysis of the electron density. The distribution of electron density values in the map is forced to be 'ideal'. The 'ideal' distribution is assumed to be Gaussian. The application of the method to improve the electron density map for the protein Acinetobacter asparaginase, which is a tetrameric enzyme of molecular weight 140000 daltons, is described. (orig.)

  2. Assembly and method for drying a product

    NARCIS (Netherlands)

    Boxtel, van A.J.B.; Bartels, P.V.; Djaeni, M.; Sanders, J.P.M.; Straten, van G.

    2008-01-01

    The application relates to an assembly for drying a product, comprising at least on drying device and at least one adsorption device, in which the adsorption device is provided with regenerable adsorption material for extracting water from a gas and comprises a gas inlet and a gas outlet, and is

  3. Production studies and documentary participants: a method

    NARCIS (Netherlands)

    Sanders, Willemien

    2016-01-01

    It was only after I finished my PhD thesis that I learned that my research related to production studies. Departing from the question of ethics in documentary filmmaking, I investigated both the perspective of filmmakers and participants on ethical issues in the documentary filmmaking practice,

  4. The effect of prices on nutrition: Comparing the impact of product- and nutrient-specific taxes.

    Science.gov (United States)

    Harding, Matthew; Lovenheim, Michael

    2017-05-01

    This paper provides an analysis of the role of prices in determining food purchases and nutrition using very detailed transaction-level observations for a large, nationally-representative sample of US consumers over the period 2002-2007. Using product-specific nutritional information, we develop a new method of partitioning the product space into relevant nutritional clusters that define a set of nutritionally-bundled goods, which parsimoniously characterize consumer choice sets. We then estimate a large utility-derived demand system over this joint product-nutrient space that allows us to calculate price and expenditure elasticities. Using our structural demand estimates, we simulate the role of product taxes on soda, sugar-sweetened beverages, packaged meals, and snacks, and nutrient taxes on fat, salt, and sugar. We find that a 20% nutrient tax has a significantly larger impact on nutrition than an equivalent product tax, due to the fact that these are broader-based taxes. However, the costs of these taxes in terms of consumer utility are only about 70 cents per household per day. A sugar tax in particular is a powerful tool to induce healthier nutritive bundles among consumers. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Optimization of technical specifications by use of probabilistic methods

    International Nuclear Information System (INIS)

    Laakso, K.

    1990-01-01

    The Technical Specifications of a nuclear power plant specify the limits for plant operation from the safety point of view. These operational safety rules were originally defined on the basis of deterministic analyses and engineering judgement. As experience has accumulated, it has proved necessary to consider problems and make specific modifications in these rules. Developments in probabilistic safety assessment have provided a new tool to analyse, present and compare the risk effects of proposed rule modificatons. The main areas covered in the project are operational decisions in failure situations, preventive maintenance during power operation and surveillance tests of standby safety systems. (author)

  6. Defining defect specifications to optimize photomask production and requalification

    Science.gov (United States)

    Fiekowsky, Peter

    2006-10-01

    Reducing defect repairs and accelerating defect analysis is becoming more important as the total cost of defect repairs on advanced masks increases. Photomask defect specs based on printability, as measured on AIMS microscopes has been used for years, but the fundamental defect spec is still the defect size, as measured on the photomask, requiring the repair of many unprintable defects. ADAS, the Automated Defect Analysis System from AVI is now available in most advanced mask shops. It makes the use of pure printability specs, or "Optimal Defect Specs" practical. This software uses advanced algorithms to eliminate false defects caused by approximations in the inspection algorithm, classify each defect, simulate each defect and disposition each defect based on its printability and location. This paper defines "optimal defect specs", explains why they are now practical and economic, gives a method of determining them and provides accuracy data.

  7. Methods for Stem Cell Production and Therapy

    Science.gov (United States)

    Valluri, Jagan V. (Inventor); Claudio, Pier Paolo (Inventor)

    2015-01-01

    The present invention relates to methods for rapidly expanding a stem cell population with or without culture supplements in simulated microgravity conditions. The present invention relates to methods for rapidly increasing the life span of stem cell populations without culture supplements in simulated microgravity conditions. The present invention also relates to methods for increasing the sensitivity of cancer stem cells to chemotherapeutic agents by culturing the cancer stem cells under microgravity conditions and in the presence of omega-3 fatty acids. The methods of the present invention can also be used to proliferate cancer cells by culturing them in the presence of omega-3 fatty acids. The present invention also relates to methods for testing the sensitivity of cancer cells and cancer stem cells to chemotherapeutic agents by culturing the cancer cells and cancer stem cells under microgravity conditions. The methods of the present invention can also be used to produce tissue for use in transplantation by culturing stem cells or cancer stem cells under microgravity conditions. The methods of the present invention can also be used to produce cellular factors and growth factors by culturing stem cells or cancer stem cells under microgravity conditions. The methods of the present invention can also be used to produce cellular factors and growth factors to promote differentiation of cancer stem cells under microgravity conditions.

  8. Research of coincidence method for calculation model of the specific detector

    Energy Technology Data Exchange (ETDEWEB)

    Guangchun, Hu; Suping, Liu; Jian, Gong [China Academy of Engineering Physics, Mianyang (China). Inst. of Nuclear Physics and Chemistry

    2003-07-01

    The physical size of specific detector is known normally, but production business is classified for some sizes that is concerned with the property of detector, such as the well diameter, well depth of detector and dead region. The surface source of even distribution and the sampling method of source particle isotropy sport have been established with the method of Monte Carlo, and gamma ray respond spectral with the {sup 152}Eu surface source been calculated. The experiment have been performed under the same conditions. Calculation and experiment results are compared with relative efficiency coincidence method and spectral similar degree coincidence method. According to comparison as a result, detector model is revised repeatedly to determine the calculation model of detector and to calculate efficiency of detector and spectra. (authors)

  9. Maize variety and method of production

    Science.gov (United States)

    Pauly, Markus; Hake, Sarah; Kraemer, Florian J

    2014-05-27

    The disclosure relates to a maize plant, seed, variety, and hybrid. More specifically, the disclosure relates to a maize plant containing a Cal-1 allele, whose expression results in increased cell wall-derived glucan content in the maize plant. The disclosure also relates to crossing inbreds, varieties, and hybrids containing the Cal-1 allele to produce novel types and varieties of maize plants.

  10. Formal methods in design and verification of functional specifications

    International Nuclear Information System (INIS)

    Vaelisuo, H.

    1995-01-01

    It is claimed that formal methods should be applied already when specifying the functioning of the control/monitoring system, i.e. when planning how to implement the desired operation of the plant. Formal methods are seen as a way to mechanize and thus automate part of the planning. All mathematical methods which can be applied on related problem solving should be considered as formal methods. Because formal methods can only support the designer, not replace him/her, they must be integrated into a design support tool. Such a tool must also aid the designer in getting the correct conception of the plant and its behaviour. The use of a hypothetic design support tool is illustrated to clarify the requirements such a tool should fulfill. (author). 3 refs, 5 figs

  11. A novel data mining method to identify assay-specific signatures in functional genomic studies

    Directory of Open Access Journals (Sweden)

    Guidarelli Jack W

    2006-08-01

    Full Text Available Abstract Background: The highly dimensional data produced by functional genomic (FG studies makes it difficult to visualize relationships between gene products and experimental conditions (i.e., assays. Although dimensionality reduction methods such as principal component analysis (PCA have been very useful, their application to identify assay-specific signatures has been limited by the lack of appropriate methodologies. This article proposes a new and powerful PCA-based method for the identification of assay-specific gene signatures in FG studies. Results: The proposed method (PM is unique for several reasons. First, it is the only one, to our knowledge, that uses gene contribution, a product of the loading and expression level, to obtain assay signatures. The PM develops and exploits two types of assay-specific contribution plots, which are new to the application of PCA in the FG area. The first type plots the assay-specific gene contribution against the given order of the genes and reveals variations in distribution between assay-specific gene signatures as well as outliers within assay groups indicating the degree of importance of the most dominant genes. The second type plots the contribution of each gene in ascending or descending order against a constantly increasing index. This type of plots reveals assay-specific gene signatures defined by the inflection points in the curve. In addition, sharp regions within the signature define the genes that contribute the most to the signature. We proposed and used the curvature as an appropriate metric to characterize these sharp regions, thus identifying the subset of genes contributing the most to the signature. Finally, the PM uses the full dataset to determine the final gene signature, thus eliminating the chance of gene exclusion by poor screening in earlier steps. The strengths of the PM are demonstrated using a simulation study, and two studies of real DNA microarray data – a study of

  12. Detection of radiolytic products by electrochemical methods

    International Nuclear Information System (INIS)

    Gonzalez-Velasco, J.

    1979-01-01

    This study demonstrates that the values of the rest potential of a platinum electrode submerged in an alkaline electrolyte (similar behaviour is also observed for an acidic one), can give a qualitative idea of the predominance in solution of radicaloid products that are oxidant and reducing in character. A simultaneous recording of potential sweep diagrams gives more precise information on the presence and characteristics of these radicals. (author)

  13. Histogram specification as a method of density modification

    Energy Technology Data Exchange (ETDEWEB)

    Harrison, R.W.

    1988-12-01

    A new method for improving the quality and extending the resolution of Fourier maps is described. The method is based on a histogram analysis of the electron density. The distribution of electron density values in the map is forced to be 'ideal'. The 'ideal' distribution is assumed to be Gaussian. The application of the method to improve the electron density map for the protein Acinetobacter asparaginase, which is a tetrameric enzyme of molecular weight 140000 daltons, is described.

  14. Use of probabilistic methods for the improvement of technical specifications

    International Nuclear Information System (INIS)

    Gros, G.; Mattei, J.M.

    1987-11-01

    The technical specifications set the requirements related to the availability of equipment. In order to limit the risk when an unavailability is noticed during power operation, an allowed outage time is determined based on a fixed limit imposed on the increase of calculated core melt probability when the equipment is considered as unavailable. An example related to the main electric equipment is presented. In case of cold shutdown condition, the operations carried out lead to numerous programmed unavailabilities of equipment. For the examination of a project of technical specifications defining the necessary equipment in the diffrent phases encountered, evaluation of the increase of the core melt probability due to the unavaibilities has been made

  15. A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.

    Directory of Open Access Journals (Sweden)

    Akos Gellért

    Full Text Available Potential porcine circovirus type 2 (PCV2 capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines.

  16. A cucumber mosaic virus based expression system for the production of porcine circovirus specific vaccines.

    Science.gov (United States)

    Gellért, Akos; Salánki, Katalin; Tombácz, Kata; Tuboly, Tamás; Balázs, Ervin

    2012-01-01

    Potential porcine circovirus type 2 (PCV2) capsid protein epitopes, suitable for expression on the surface of cucumber mosaic virus (CMV) particles were determined by a thorough analysis of the predicted PCV capsid protein structure. The ab initio protein structure prediction was carried out with fold recognition and threading methods. The putative PCV epitopes were selected on the basis of PCV virion models and integrated into the plant virus coat protein, after amino acid position 131. The recombinants were tested for infectivity and stability on different Nicotiana species and stable recombinant virus particles were purified. The particles were tested for their ability to bind to PCV induced porcine antibodies and used for specific antibody induction in mice and pigs. The results showed that PCV epitopes expressed on the CMV surface were recognized by the porcine antibodies and they were also able to induce PCV specific antibody response. Challenge experiment with PCV2 carried out in immunized pigs showed partial protection against the infection. Based on these results it was concluded that specific antiviral vaccine production for the given pathogen was feasible, offering an inexpensive way for the mass production of such vaccines.

  17. Waste classification and methods applied to specific disposal sites

    International Nuclear Information System (INIS)

    Rogers, V.C.

    1979-01-01

    An adequate definition of the classes of radioactive wastes is necessary to regulating the disposal of radioactive wastes. A classification system is proposed in which wastes are classified according to characteristics relating to their disposal. Several specific sites are analyzed with the methodology in order to gain insights into the classification of radioactive wastes. Also presented is the analysis of ocean dumping as it applies to waste classification. 5 refs

  18. Tracer preparate and method for its production

    International Nuclear Information System (INIS)

    Pratt, F.P.; Gagnon, D.

    1978-01-01

    The injectable tracer preparate for investigations to determine the blood flow in organs or the effect of drugs on the blood flow consists of a core of ion exchanger resin coated with polyfuran or a polymer which is the reaction product of a monomer catalysable by acid or base. The nuclei have a diameter of 10 to 200 micron, the coating thickness is between 1 and 3 micron. Ions of Ce 141, Cr 51, Sr 85, Sr 46 or Co 57 of strength 0.1-100 millicurie are adsorbed on the nucleus. (DG) [de

  19. Comparative Evaluations of Four Specification Methods for Real-Time Systems

    Science.gov (United States)

    1989-12-01

    December 1989 Comparative Evaluations of Four Specification Methods for Real - Time Systems David P. Wood William G. Wood Specification and Design Methods...Methods for Real - Time Systems Abstract: A number of methods have been proposed in the last decade for the specification of system and software requirements...and software specification for real - time systems . Our process for the identification of methods that meet the above criteria is described in greater

  20. Methods and systems for chemoautotrophic production of organic compounds

    Energy Technology Data Exchange (ETDEWEB)

    Fischer, Curt R.; Che, Austin J.; Shetty, Reshma P.; Kelly, Jason R.

    2018-02-27

    The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

  1. 40 CFR 268.20 - Waste specific prohibitions-Dyes and/or pigments production wastes.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 26 2010-07-01 2010-07-01 false Waste specific prohibitions-Dyes and/or pigments production wastes. 268.20 Section 268.20 Protection of Environment ENVIRONMENTAL... Disposal § 268.20 Waste specific prohibitions—Dyes and/or pigments production wastes. (a) Effective August...

  2. Method for production of transparent yttrium oxide

    International Nuclear Information System (INIS)

    Dutta, S.K.; Gazza, G.A.

    1975-01-01

    The method comprises vacuum hot pressing the yttrium oxide (Y 2 O 3 ) powder in a graphite die at temperatures of between 1300 to 1500 0 C and uniaxial pressures of between 5000 to 7000 psi, for a period of 1 to 2 hours. (U.S.)

  3. Method of production multifilamentary intermetallic superconductors

    International Nuclear Information System (INIS)

    Marancik, W.G.; Young, M.S.

    1980-01-01

    A method of making A-15 type intermetallic superconductors is disclosed which features elimination of numerous annealing steps. Nb or V filaments are embedded in Cu matrices; annular layers of Sn or Ga, respectively, separated from each other by Cu layers, provide the other component of the intermetallic superconductors Nb3Sn and V3Ga

  4. Crop-Specific Grafting Methods, Rootstocks and Scheduling-Tomato

    Science.gov (United States)

    Grafting has gained popularity as a method to manage plant diseases previously controlled by soil fumigation with methyl bromide. Some of the most significant soilborne pest problems for which resistant rootstocks may be beneficial include root-knot nematodes, Verticillium wilt, and southern blight....

  5. Optimizing Cofactor Specificity of Oxidoreductase Enzymes for the Generation of Microbial Production Strains—OptSwap

    DEFF Research Database (Denmark)

    King, Zachary A.; Feist, Adam

    2013-01-01

    Central oxidoreductase enzymes (eg, dehydrogenases, reductases) in microbial metabolism often have preferential binding specificity for one of the two major currency metabolites NAD(H) and NADP(H). These enzyme specificities result in a division of the metabolic functionality of the currency...... specificities of oxidoreductase enzyme and complementary reaction knockouts. Using the Escherichia coli genome-scale metabolic model iJO1366, OptSwap predicted eight growth-coupled production designs with significantly greater product yields or substrate-specific productivities than designs predicted with gene...

  6. Foam, composition and method of production

    Energy Technology Data Exchange (ETDEWEB)

    Ghali, R.

    1991-09-03

    A ternary foam is disclosed which overcomes the disadvantages of conventional heat-insulating foams which are either dense and heavy, expensive to produce, friable, unstable, or have poor fire resistance. The foam of the invention has, by weight of the reaction product, 10-35% polyurethane, 30-55% polyisocyanurate, and 20-45% polyurea. The foam is a reaction product of 100 parts by weight of an organic polyisocyanate with: 15-25 parts by weight of a polyether polyol having a hydroxyl number of ca 28-35 and a molecular weight of not more than 6,000; 5-25 parts by weight of water; 0-25 parts by weight of a blowing agent; 0-50 parts by weight of a filler-plasticizer fire retardant; an effective amount of a low activation catalyst to initiate water-isocyanate reaction to produce polyurea and polyol-isocyanate reaction to produce polyurethane; and an effective amount of a high activation catalyst to retard the low activation catalyst and promote the formation of polyisocyanurate. The low activity catalyst is present in an amount ranging ca 1.5-9 parts per 100 parts by weight of the polyisocyanate, and the high activation catalyst is present in an amount ranging 0.0008-0.002 parts per 100 parts by weight of the polyisocyanate. Water as a reactant and blowing agent can be substituted by an effective amount of a disubstituted amine such as diethanol amine and an inert blowing agent such as fluorocarbon. The resultant foam has excellent heat insulating propeties, is semi-flexible, has a density of 0.5-0.9 lb/ft{sup 3}, and can be inexpensively produced by existing 2-component foam dispensing equipment. 1 fig., 1 tab.

  7. Biochar production method and composition therefrom

    Science.gov (United States)

    Lee, James W.; Buchanan, III, Archibald C.; Evans, Barbara R.; Kidder, Michelle K.

    2013-03-19

    The invention is directed to a method for producing an oxygenated biochar material possessing a cation-exchanging property, wherein a biochar source is reacted with one or more oxygenating compounds in such a manner that the biochar source homogeneously acquires oxygen-containing cation-exchanging groups in an incomplete combustion process. The invention is also directed to oxygenated biochar compositions and soil formulations containing the oxygenated biochar material.

  8. Cardiac pacemaker and its production methods

    International Nuclear Information System (INIS)

    Purdy, D.L.; Speicher, V.; Shipko, F.J.; Johnson, W.L.

    1977-01-01

    The construction method of the cardiac pacemakers proposes to arrange the impulse machine and the battery between two container halves, to weld the container halves with the help of a laser beam under a protective atmosphere and finally to inject polyurethane foam through an orifice into the form. There is also the possibility to embed the impulse machine and the battery first into the polyurethane foam and afterwards to close both container halves. Titanium is a suitable material for the container. (DG) [de

  9. Method for production of dicarbonic acid anhydrides

    International Nuclear Information System (INIS)

    Mistr, A.; Necas, J.; Polak, V.

    1975-01-01

    A method is described of producing dicarboxylic acid anhydrides by the reaction of maleic acid anhydride with olefins. The synthesis is initiated by gamma radiation at a total dose of 10 4 to 10 6 rads in the presence of an organic solvent. The addition reactions of maleic acid anhydride to 1-hexadecene, 1-octene and cyclohexene with yields of 43%, 17% and 11%, respectively, are specified. (L.K.)

  10. Methodological proposal for environmental impact evaluation since different specific methods

    International Nuclear Information System (INIS)

    Leon Pelaez, Juan Diego; Lopera Arango Gabriel Jaime

    1999-01-01

    Some conceptual and practical elements related to environmental impact evaluation are described and related to the preparation of technical reports (environmental impact studies and environmental management plans) to be presented to environmental authorities for obtaining the environmental permits for development projects. In the first part of the document a summary of the main aspects of normative type is made that support the studies of environmental impact in Colombia. We propose a diagram for boarding and elaboration of the evaluation of environmental impact, which begins with the description of the project and of the environmental conditions in the area of the same. Passing then to identify the impacts through a method matricial and continuing with the quantitative evaluation of the same. For which we propose the use of the method developed by Arboleda (1994). Also we propose to qualify the activities of the project and the components of the environment in their relative importance, by means of a method here denominated agglomerate evaluation. Which allows finding those activities more impacting and the mostly impacted components. Lastly it is presented some models for the elaboration and presentation of the environmental management plans. The pursuit programs and those of environmental supervision

  11. Business Cycles With A Common Trend in Neutral and Investment-Specific Productivity

    OpenAIRE

    Schmitt-Grohé, Stephanie; Uribe, Martín

    2010-01-01

    This paper identifies a new source of business-cycle fluctuations. Namely, a common stochastic trend in neutral and investment-specific productivity. We document that in U.S. postwar quarterly data total factor productivity (TFP) and the relative price of investment are cointegrated. We show theoretically that TFP and the relative price of investment are cointegrated if and only if neutral and investment-specific productivity share a common stochastic trend. We econometrically estimate an RBC...

  12. Chemometrics Methods for Specificity, Authenticity and Traceability Analysis of Olive Oils: Principles, Classifications and Applications

    Directory of Open Access Journals (Sweden)

    Habib Messai

    2016-11-01

    Full Text Available Background. Olive oils (OOs show high chemical variability due to several factors of genetic, environmental and anthropic types. Genetic and environmental factors are responsible for natural compositions and polymorphic diversification resulting in different varietal patterns and phenotypes. Anthropic factors, however, are at the origin of different blends’ preparation leading to normative, labelled or adulterated commercial products. Control of complex OO samples requires their (i characterization by specific markers; (ii authentication by fingerprint patterns; and (iii monitoring by traceability analysis. Methods. These quality control and management aims require the use of several multivariate statistical tools: specificity highlighting requires ordination methods; authentication checking calls for classification and pattern recognition methods; traceability analysis implies the use of network-based approaches able to separate or extract mixed information and memorized signals from complex matrices. Results. This chapter presents a review of different chemometrics methods applied for the control of OO variability from metabolic and physical-chemical measured characteristics. The different chemometrics methods are illustrated by different study cases on monovarietal and blended OO originated from different countries. Conclusion. Chemometrics tools offer multiple ways for quantitative evaluations and qualitative control of complex chemical variability of OO in relation to several intrinsic and extrinsic factors.

  13. Chemometrics Methods for Specificity, Authenticity and Traceability Analysis of Olive Oils: Principles, Classifications and Applications

    Science.gov (United States)

    Messai, Habib; Farman, Muhammad; Sarraj-Laabidi, Abir; Hammami-Semmar, Asma; Semmar, Nabil

    2016-01-01

    Background. Olive oils (OOs) show high chemical variability due to several factors of genetic, environmental and anthropic types. Genetic and environmental factors are responsible for natural compositions and polymorphic diversification resulting in different varietal patterns and phenotypes. Anthropic factors, however, are at the origin of different blends’ preparation leading to normative, labelled or adulterated commercial products. Control of complex OO samples requires their (i) characterization by specific markers; (ii) authentication by fingerprint patterns; and (iii) monitoring by traceability analysis. Methods. These quality control and management aims require the use of several multivariate statistical tools: specificity highlighting requires ordination methods; authentication checking calls for classification and pattern recognition methods; traceability analysis implies the use of network-based approaches able to separate or extract mixed information and memorized signals from complex matrices. Results. This chapter presents a review of different chemometrics methods applied for the control of OO variability from metabolic and physical-chemical measured characteristics. The different chemometrics methods are illustrated by different study cases on monovarietal and blended OO originated from different countries. Conclusion. Chemometrics tools offer multiple ways for quantitative evaluations and qualitative control of complex chemical variability of OO in relation to several intrinsic and extrinsic factors. PMID:28231172

  14. 24 CFR 200.943 - Supplementary specific requirements under the HUD building product standards and certification...

    Science.gov (United States)

    2010-04-01

    ... under the HUD building product standards and certification program for the grademarking of lumber. 200... Supplementary specific requirements under the HUD building product standards and certification program for the... compliance with the U.S. Department of Commerce Voluntary Product Standard PS 20-94 American Softwood Lumber...

  15. 24 CFR 200.952 - Supplementary specific requirements under the HUD building product standards and certification...

    Science.gov (United States)

    2010-04-01

    ... under the HUD building product standards and certification program for particleboard interior stair... Supplementary specific requirements under the HUD building product standards and certification program for... forth in § 200.935(d)(6) concerning labeling of a product, the administrator's validation mark and the...

  16. 24 CFR 200.954 - Supplementary specific requirements under the HUD building product standard and certification...

    Science.gov (United States)

    2010-04-01

    ... under the HUD building product standard and certification program for construction adhesives for wood... § 200.954 Supplementary specific requirements under the HUD building product standard and certification...) concerning labeling of a product, the administrator's validation mark and the manufacturer's certification of...

  17. 24 CFR 200.936 - Supplementary specific procedural requirements under HUD building products certification program...

    Science.gov (United States)

    2010-04-01

    ... requirements under HUD building products certification program for solid fuel type room heaters and fireplace... Supplementary specific procedural requirements under HUD building products certification program for solid fuel... fireplace stoves certified under the HUD Building Products Certification Program shall be designed...

  18. Production of Molybdenum-99 using Neutron Capture Methods

    Energy Technology Data Exchange (ETDEWEB)

    Toth, James J; Greenwood, Lawrence R; Soderquist, Chuck Z; Wittman, Richard S; Pierson, Bruce D; Burns, Kimberly A; Lavender, Curt A; Painter, Chad L; Love, Edward F; Wall, Donald E

    2011-01-01

    Pacific Northwest National Laboratory (PNNL), operated by Battelle, has identified a reference process for the production of molybdenum-99 (99Mo) for use in a chromatographic generator to separate the daughter product, technetium-99m (99mTc). The reference process uses the neutron capture reaction of natural or enriched molybdenum oxide via the reaction 98Mo(n,γ)99Mo. The irradiated molybdenum is dissolved in an alkaline solution, whereby the molybdenum, dissolved as the molybdate anion, is loaded on a proprietary ion exchange material in the chromatographic generator. The approach of this investigation is to provide a systematic collection of technologies to make the neutron capture method for Mo-99 production economically viable. This approach would result in the development of a technetium Tc99m generator and a new type of target. The target is comprised of molybdenum, either natural or enriched, and is tailored to the design of currently operating U.S. research reactors. The systematic collection of technologies requires evaluation of new metallurgical methods to produce the target, evaluation of target geometries tailored to research reactors, and chemical methods to dissolve the irradiated target materials for use in a chromatographic generator. A Technical specification for testing the target and neutron capture method in a research reactor is also required. This report includes identification of research and demonstration activities needed to enable deployment of neutron capture production method, including irradiations of prototypic targets, chemical processing of irradiated targets, and loading and extraction tests of Mo99 and Tc99m on the sorbent material in a prototypic generator design. The prototypical generator design is based on the proprietary method and systems for isotope product generation. The proprietary methods and systems described in this report are clearly delineated with footnotes. Ultimately, the Tc-99m generator solution provided by

  19. Method for extracting protein from a fermentation product

    Science.gov (United States)

    Lawton, Jr., John Warren; Bootsma, Jason Alan; Lewis, Stephen Michael

    2014-02-18

    A method of producing bioproducts from a feedstock in a system configured to produce ethanol and distillers grains from a fermentation product is disclosed. A system configured to process feedstock into a fermentation product and bioproducts including ethanol and meal is disclosed. A bioproduct produced from a fermentation product produced from a feedstock in a biorefining system is disclosed.

  20. Methods of detection using a cellulose binding domain fusion product

    Science.gov (United States)

    Shoseyov, Oded; Shpiegl, Itai; Goldstein, Marc A.; Doi, Roy H.

    1999-01-01

    A cellulose binding domain (CBD) having a high affinity for crystalline cellulose and chitin is disclosed, along with methods for the molecular cloning and recombinant production thereof. Fusion products comprising the CBD and a second protein are likewise described. A wide range of applications are contemplated for both the CBD and the fusion products, including drug delivery, affinity separations, and diagnostic techniques.

  1. Determination of photooxygenation products of rotenone with isotope dilution method

    International Nuclear Information System (INIS)

    Chubachi, Mitsuo; Hamada, Masayuki

    1975-01-01

    When rotenone dissolved in certain solvent was photochemically oxidized, rotenolones, dehydrorotenone and rotenonone were obtained as main products. In order to determine the quantitative yields of these compounds in photooxygenation products, four compounds mentioned above were labeled with carbon-14 and the isotope dilution method by these labeled compounds was applied to the product analysis. (auth.)

  2. Recommended environmental dose calculation methods and Hanford-specific parameters

    International Nuclear Information System (INIS)

    Schreckhise, R.G.; Rhoads, K.; Napier, B.A.; Ramsdell, J.V.; Davis, J.S.

    1993-03-01

    This document was developed to support the Hanford Environmental Dose overview Panel (HEDOP). The Panel is responsible for reviewing all assessments of potential doses received by humans and other biota resulting from the actual or possible environmental releases of radioactive and other hazardous materials from facilities and/or operations belonging to the US Department of Energy on the Hanford Site in south-central Washington. This document serves as a guide to be used for developing estimates of potential radiation doses, or other measures of risk or health impacts, to people and other biota in the environs on and around the Hanford Site. It provides information to develop technically sound estimates of exposure (i.e., potential or actual) to humans or other biotic receptors that could result from the environmental transport of potentially harmful materials that have been, or could be, released from Hanford operations or facilities. Parameter values and information that are specific to the Hanford environs as well as other supporting material are included in this document

  3. Recommended environmental dose calculation methods and Hanford-specific parameters

    Energy Technology Data Exchange (ETDEWEB)

    Schreckhise, R.G.; Rhoads, K.; Napier, B.A.; Ramsdell, J.V. (Pacific Northwest Lab., Richland, WA (United States)); Davis, J.S. (Westinghouse Hanford Co., Richland, WA (United States))

    1993-03-01

    This document was developed to support the Hanford Environmental Dose overview Panel (HEDOP). The Panel is responsible for reviewing all assessments of potential doses received by humans and other biota resulting from the actual or possible environmental releases of radioactive and other hazardous materials from facilities and/or operations belonging to the US Department of Energy on the Hanford Site in south-central Washington. This document serves as a guide to be used for developing estimates of potential radiation doses, or other measures of risk or health impacts, to people and other biota in the environs on and around the Hanford Site. It provides information to develop technically sound estimates of exposure (i.e., potential or actual) to humans or other biotic receptors that could result from the environmental transport of potentially harmful materials that have been, or could be, released from Hanford operations or facilities. Parameter values and information that are specific to the Hanford environs as well as other supporting material are included in this document.

  4. Systems and methods of storing combustion waste products

    Science.gov (United States)

    Chen, Shen-En; Wang, Peng; Miao, Xiexing; Feng, Qiyan; Zhu, Qianlin

    2016-04-12

    In one aspect, methods of storing one or more combustion waste products are described herein. Combustion waste products stored by a method described herein can include solid combustion waste products such as coal ash and/or gaseous combustion products such as carbon dioxide. In some embodiments, a method of storing carbon dioxide comprises providing a carbon dioxide storage medium comprising porous concrete having a macroporous and microporous pore structure and flowing carbon dioxide captured from a combustion flue gas source into the pore structure of the porous concrete.

  5. Some non-thermal microbial inactivation methods in dairy products

    International Nuclear Information System (INIS)

    Yangilar, F.; Kabil, E.

    2013-01-01

    During the production of dairy products, some thermal processes such as pasteurization and sterilization are used commonly to inactive microorganisms. But as a result of thermal processes, loss of nutrient and aroma, non-enzymatic browning and organoleptic differentiation especially in dairy products are seen. Because of this, alternative methods are needed to provide microbial inactivation and as major problems are caused by high temperatures, non-thermal processes are focused on. For this purpose, some methods such as high pressure (HP), pulsed light (PL), ultraviolet radiation (UV), supercritical carbon dioxide (SC-CO2) or pulsed electric field (PEF) are used in food. These methods products are processed in ambient temperature and so not only mentioned losses are minimized but also freshness and naturality of products can be preserved. In this work, we will try to be given information about methods of non-thermal microbial inactivation of dairy products. (author) [tr

  6. Remote methods of indicating oil products in natural waters

    Energy Technology Data Exchange (ETDEWEB)

    Shlyakhova, L A

    1981-01-01

    A survey is made of domestic and foreign publications covering remote methods of monitoring film petroleum products and oil in natural waters. The given methods are realized in practice with the use of different sections of the electromagnetic spectrum. Remote quality control of the natural waters at the modern level may be an indicator of water pollution with film petroleum products.

  7. Generating and testing methods for consumer-oriented product development

    International Nuclear Information System (INIS)

    2001-10-01

    In order to obtain a good insight into various design methods that can be used by product developers to enable them to develop and test useful domotics products (domotics: intelligent systems for the home), an inventory has been made of the methods used in the Netherlands. The inventory is directed at two categories of methods: (1) Methods of getting better acquainted with the user and/or the problem, and of generating novel solutions: generative methods; and (2) Methods of assessing solutions (through various phases of the designing process): testing methods. The first category of methods concentrates on the designing process. In other words: how can the designer realise as much as possible of the workability of (domotics) products during the designing process? The second category aims at testing a design (in whatever shape: drawing, prototype, functional computer animation, etc.) through its users. These are methods of assessing a design at various stages of the designing process [nl

  8. Methods for production of aluminium powders and their application fields

    Energy Technology Data Exchange (ETDEWEB)

    Gopienko, V.G.; Kiselev, V.P.; Zobnina, N.S. (Vsesoyuznyj Nauchno-Issledovatel' skij i Proektnyj Inst. Alyuminievoj, magnievoj i ehlektrodnoj promyshlennosti (USSR))

    1984-12-01

    Different types of powder products made of alluminium and its alloys (powder, fine powders, granules and pastes) as well as their basic physicochemical properties are briefly characterized. The principle methods for alluminium powder production are outlined: physicochemical methods, the melt spraying by compressed gas being the mostly developed among them, and physico-mechanical ones. Main application spheres for powder productions of aluminium and its alloys are reported in short.

  9. Methods for production of aluminium powders and their application fields

    International Nuclear Information System (INIS)

    Gopienko, V.G.; Kiselev, V.P.; Zobnina, N.S.

    1984-01-01

    Different types of powder products made of alluminium and its alloys (powder, fine powders, granules and pastes) as well as their basic physicochemical properties are briefly characterized. The principle methods for alluminium powder production are outlined: physicochemical methods, the melt spraying by compressed gas being the mostly developed among them, and physico-mechanical ones. Main application spheres for powder productions of aluminium and its alloys are reported in short

  10. Neutron absorbers, and the production method

    International Nuclear Information System (INIS)

    Kayano, Hideo; Yajima, Seishi; Oono, Hironori.

    1979-01-01

    Purpose: To integrally sinter a metal powder and a metal network material thereby to obtain a material having a high neutron absorbing function, an excellent corrosion resistance and an excellent oxidation resistance. Method: An element having a high neutron absorbing function, such as Gd, or a compound thereof and a powder of a metal having excellent corrosion resistance, oxidation resistance and ductility, such as Fe, Cr or the like are uniformly mixed with each other. In a case where a substance having a neutron absorbing function is a hydroxide an organic complex or the like, it is formed into a gel-like substance and mixed uniformly with the metal powder, the gel-like substance being pasted, and covered on the surface of the metal powder and dried. Then, the mixture or the dry coated material is extended and the metal network material having excellent corrosion resistance, oxidation resistance and ductility is covered or interposed or between at least one layer of upper, intermediate or lower layers of said laminated material, and thereafter is subjected to cold or hot rolling, and then sintered and furthermore rolled, if necessary, the thus treated material being burned in vacuum or a non-oxidizing atmosphere. (Kamimura, M.)

  11. A Method for User Centering Systematic Product Development Aimed at Industrial Design Students

    Science.gov (United States)

    Coelho, Denis A.

    2010-01-01

    Instead of limiting the introduction and stimulus for new concept creation to lists of specifications, industrial design students seem to prefer to be encouraged by ideas in context. A new method that specifically tackles human activity to foster the creation of user centered concepts of new products was developed and is presented in this article.…

  12. A soil-specific agro-ecological strategy for sustainable production in Argentina farm fields

    Science.gov (United States)

    Zamora, Martin; Barbera, Agustin; Castro-Franco, Mauricio; Hansson, Alejandro; Domenech, Marisa

    2017-04-01

    The continuous increment of frequencies and doses of pesticides, glyphosate and fertilizers, the deterioration of the structure, biotic balance and fertility of soils and the ground water pollution are characteristics of the current Argentinian agricultural model. In this context, agro-ecological innovations are needed to develop a real sustainable agriculture, enhancing the food supply. Precision agriculture technologies can strengthen the expansion of agro-ecological farming in experimental farm fields. The aim of this study was to propose a soil-specific agro-ecological strategy for sustainable production at field scale focused on the use of soil sensors and digital soil mapping techniques. This strategy has been developed in 15 hectares transition agro-ecological farm field, located at Barrow Experimental Station (Lat:-38.322844, Lon:-60.25572) Argentina. The strategy included five steps: (i) to measure apparent electrical conductivity (ECa) and elevation within agro-ecological farm field; (ii) to apply a clustering method using MULTISPATI-PCA algorithm to delimitate three soil-specific zones (Z1, Z2 and Z3); (iii) to determine three soil sampling points by zone, using conditioned Latin hypercube method, in addition to elevation and ECa as auxiliary information; (iv) to collect soil samples at 2-10 cm depth in each point and to determine in laboratory: total organic carbon content (TOC), cation-exchange capacity (CEC), pH and phosphorus availability (P-Bray). In addition, soil bulk density (SBD) was measured at 0-20 cm depth. Finally, (v) according to each soil-specific zone, a management strategy was recommended. Important differences in soil properties among zones could suggest that the strategy developed was able to apply an agro ecological soil-specific practice management. pH and P-Bray were significantly (pfertilizer and also rotating plots with high stocking rate. The aim is to increase soil organic matter content and CEC. Furthermore, P content will be

  13. Layered Electrical Product Application Protocol (AP). Draft: Initial Graphics Exchange Specification (IGES)

    Energy Technology Data Exchange (ETDEWEB)

    1994-09-01

    An application protocol is an information systems engineering view of a specific product. The view represents an agreement on the generic activities needed to design and fabricate the product, the agreement on the information needed to support those activities, and the specific constructs of a product data standard for use in transfering some or all of the information required. This applications protocol describes the data for electrical and electronic products in terms of a product description standard called the Initial Graphics Exchange Specification (IGES). More specifically, the Layered Electrical Product IGES Application Protocol (AP) specifies the mechanisms for defining and exchanging computer-models and their associated data for those products which have been designed in two dimensional geometry so as to be produced as a series of layers in IGES format. The AP defines the appropriateness of the data items for describing the geometry of the various parts of a product (shape and location), the connectivity, and the processing and material characteristics. Excluded is the behavioral requirements which the product was intended to satisfy, except as those requirements have been recorded as design rules or product testing requirements.

  14. Nondestructive methods for quality evaluation of livestock products.

    Science.gov (United States)

    Narsaiah, K; Jha, Shyam N

    2012-06-01

    The muscles derived from livestock are highly perishable. Rapid and nondestructive methods are essential for quality assurance of such products. Potential nondestructive methods, which can supplement or replace many of traditional time consuming destructive methods, include colour and computer image analysis, NIR spectroscopy, NMRI, electronic nose, ultrasound, X-ray imaging and biosensors. These methods are briefly described and the research work involving them for products derived from livestock is reviewed. These methods will be helpful in rapid screening of large number of samples, monitoring distribution networks, quick product recall and enhance traceability in the value chain of livestock products. With new developments in the areas of basic science related to these methods, colour, image processing, NIR spectroscopy, biosensors and ultrasonic analysis are expected to be widespread and cost effective for large scale meat quality evaluation in near future.

  15. Specificity of Correlation Pattern Recognition Methods Application in Security Holograms Identity Control Apparatus

    Science.gov (United States)

    Zlokazov, E. Yu.; Starikov, R. S.; Odinokov, S. B.; Tsyganov, I. K.; Talalaev, V. E.; Koluchkin, V. V.

    Automatic inspection of security hologram (SH) identity is highly demanded issue due high distribution of SH worldwide to protect documents such as passports, driving licenses, banknotes etc. While most of the known approaches use inspection of SH design features none of these approaches inspect the features of its surface relief that is a direct contribution to original master matrix used for these holograms production. In our previous works we represented the device that was developed to provide SH identification by processing of coherent responses of its surface elements. Most of the algorithms used in this device are based on application of correlation pattern recognition methods. The main issue of the present article is a description of these methods application specificities.

  16. method and container for production of diagnostic scanning agents

    International Nuclear Information System (INIS)

    Ruddock, C.F.

    1979-01-01

    The pertechnetate ion containing the technetium-99m isotope has limited applications in diagnostic scanning because it does not readily form complexes with materials which locate in specific parts of the body. Stannous salts have been widely used to reduce the pertechnetate to a form which readily complexes with materials. In the present invention, both a container and a more suitable metal reducing agent are discussed for transforming the technetium in pertechnetate for diagnostic scanning use. The vessel contains tin or a tin-containing alloy as a reducing agent for the pertechnetate and a complexant for the reduced technetium; all contents are sterile and dry. The present invention is advantageous over the stannous salts method since (1) problems of stannous salt instability during production, storage and after labelling are eliminated; (2) production procedures are simplified; (3) it is not essential to nitrogen purge vials before sterilisation; (4) it reduces toxicity; (5) the shelf life of diagnostic scanning kits may be dramatically improved; (6) the metal reducing agent may be sterilised by γ-irradiation without deteriorating; (7) the labelling technique can be performed over a wide pH range; and (8) the technique should be unaffected by technetium-99 in the technetium-99m. (U.K.)

  17. The need for an established allocation method when assessing absolute sustainability on a product level

    DEFF Research Database (Denmark)

    Ryberg, Morten; Owsianiak, Mikolaj; Hauschild, Michael Zwicky

    2015-01-01

    Assessment of absolute sustainability within life cycle assessment (LCA) framework is operational on the country scale. However, it is difficult to apply the existing approaches to products, which are typically the scope of LCAs. How should we assess whether a chair is (absolutely) sustainable? I...... allocation keys specific to each product group, e.g. mass for furniture, or economic revenue for IT. The proposed method facilitates assessment of absolute sustainability of products within the LCA framework....

  18. Using non-parametric methods in econometric production analysis

    DEFF Research Database (Denmark)

    Czekaj, Tomasz Gerard; Henningsen, Arne

    Econometric estimation of production functions is one of the most common methods in applied economic production analysis. These studies usually apply parametric estimation techniques, which obligate the researcher to specify the functional form of the production function. Most often, the Cobb...... results—including measures that are of interest of applied economists, such as elasticities. Therefore, we propose to use nonparametric econometric methods. First, they can be applied to verify the functional form used in parametric estimations of production functions. Second, they can be directly used...

  19. Estimation of product specific emissions from municipal solid waste landfills for the inventory phase in LCA

    DEFF Research Database (Denmark)

    Nielsen, Per Henning; Hauschild, Michael Zwicky

    1998-01-01

    is frequently given as a quantity of solid wasteand possibly some recovered energy from waste incineration.Since product specific emissions can not be calculated or measured directly at the landfills, they must be estimated by modeling oflandfill processes. This paper presents a landfill model based on a large......), and inorganic non-metals (e.g. chlorine,) which are considered individually. The computer toolLCA-LAND is useful for estimation of emissions from specific waste products disposed in municipal solid waste landfills in Europeancountries (for the present Denmark, Germany and The Netherlands). Input data...... of materials and components and the manufacture, transportation and use of the product to thefinal disposal and possible recycling of the product. Although LCA has developed significantly during recent years, product specific emissions from disposed waste have only got minorattention in the literature leaving...

  20. [Combine fats products: methodic opportunities of it identification].

    Science.gov (United States)

    Viktorova, E V; Kulakova, S N; Mikhaĭlov, N A

    2006-01-01

    At present time very topical problem is falsification of milk fat. The number of methods was considered to detection of milk fat authention and possibilities his difference from combined fat products. The analysis of modern approaches to valuation of milk fat authention has showed that the main method for detection of fat nature is gas chromatography analysis. The computer method of express identification of fat products is proposed for quick getting of information about accessory of examine fat to nature milk or combined fat product.

  1. Method of processing solidification product of radioactive waste

    International Nuclear Information System (INIS)

    Daime, Fumiyoshi.

    1988-01-01

    Purpose: To improve the long-time stability of solidification products by providing solidification products with liquid tightness, gas tightness, abrasion resistance, etc., of the products in the course of the solidification for the treatment of radioactive wastes. Method: The surface of solidification products prepared by mixing solidifying agents with powder or pellets is entirely covered with high molecular polymer such as epoxy resin. The epoxy resin has excellent properties such as radiation-resistance, heat resistance, water proofness and chemical resistance, as well as have satisfactory mechanical properties. This can completely isolate the solidification products of radioactive wastes from the surrounding atmosphere. (Yoshino, Y.)

  2. End product inhibition of hepatic 25-hydroxyvitamin D production in the rat: specificity and kinetics

    International Nuclear Information System (INIS)

    Milne, M.L.; Baran, D.T.

    1985-01-01

    The role of vitamin D metabolites in the regulation of hepatic 25-hydroxyvitamin D production was investigated by examining the effects of 25-hydroxyvitamin D, 1,25-dihydroxyvitamin D, and 24,25-dihydroxyvitamin D on the synthesis of [25- 3 H]hydroxyvitamin D by rachitic rat liver homogenates. Production of [25- 3 H]hydroxyvitamin D was inhibited by 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D, but not by 24,25-dihydroxyvitamin D. 25-Hydroxyvitamin D increased the Km of the vitamin D-25-hydroxylase enzyme(s), while 1,25-dihydroxyvitamin D decreased the Vmax with a Ki of 88.7 ng/ml. Inhibition of hepatic 25-hydroxyvitamin D production by 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D may be another control mechanism to regulate circulating vitamin D levels

  3. Time-trends in method-specific suicide rates compared with the availability of specific compounds. The Danish experience

    DEFF Research Database (Denmark)

    Nordentoft, Merete; Qin, Ping; Helweg-Larsen, Karin

    2006-01-01

    Restriction of means for suicide is an important part of suicide preventive strategies in different countries. All suicides in Denmark between 1970 and 2000 were examined with regard to method used for suicide. Overall suicide mortality and method-specific suicide mortality was compared...... in the number of suicides by self-poisoning with these compounds. Restricted access occurred concomittantly with a 55% decrease in suicide rate...

  4. Comparison of methods for estimating carbon in harvested wood products

    International Nuclear Information System (INIS)

    Claudia Dias, Ana; Louro, Margarida; Arroja, Luis; Capela, Isabel

    2009-01-01

    There is a great diversity of methods for estimating carbon storage in harvested wood products (HWP) and, therefore, it is extremely important to agree internationally on the methods to be used in national greenhouse gas inventories. This study compares three methods for estimating carbon accumulation in HWP: the method suggested by Winjum et al. (Winjum method), the tier 2 method proposed by the IPCC Good Practice Guidance for Land Use, Land-Use Change and Forestry (GPG LULUCF) (GPG tier 2 method) and a method consistent with GPG LULUCF tier 3 methods (GPG tier 3 method). Carbon accumulation in HWP was estimated for Portugal under three accounting approaches: stock-change, production and atmospheric-flow. The uncertainty in the estimates was also evaluated using Monte Carlo simulation. The estimates of carbon accumulation in HWP obtained with the Winjum method differed substantially from the estimates obtained with the other methods, because this method tends to overestimate carbon accumulation with the stock-change and the production approaches and tends to underestimate carbon accumulation with the atmospheric-flow approach. The estimates of carbon accumulation provided by the GPG methods were similar, but the GPG tier 3 method reported the lowest uncertainties. For the GPG methods, the atmospheric-flow approach produced the largest estimates of carbon accumulation, followed by the production approach and the stock-change approach, by this order. A sensitivity analysis showed that using the ''best'' available data on production and trade of HWP produces larger estimates of carbon accumulation than using data from the Food and Agriculture Organization. (author)

  5. Validation of Land Cover Products Using Reliability Evaluation Methods

    Directory of Open Access Journals (Sweden)

    Wenzhong Shi

    2015-06-01

    Full Text Available Validation of land cover products is a fundamental task prior to data applications. Current validation schemes and methods are, however, suited only for assessing classification accuracy and disregard the reliability of land cover products. The reliability evaluation of land cover products should be undertaken to provide reliable land cover information. In addition, the lack of high-quality reference data often constrains validation and affects the reliability results of land cover products. This study proposes a validation schema to evaluate the reliability of land cover products, including two methods, namely, result reliability evaluation and process reliability evaluation. Result reliability evaluation computes the reliability of land cover products using seven reliability indicators. Process reliability evaluation analyzes the reliability propagation in the data production process to obtain the reliability of land cover products. Fuzzy fault tree analysis is introduced and improved in the reliability analysis of a data production process. Research results show that the proposed reliability evaluation scheme is reasonable and can be applied to validate land cover products. Through the analysis of the seven indicators of result reliability evaluation, more information on land cover can be obtained for strategic decision-making and planning, compared with traditional accuracy assessment methods. Process reliability evaluation without the need for reference data can facilitate the validation and reflect the change trends of reliabilities to some extent.

  6. Crystal structure of inulosucrase from Lactobacillus: insights into the substrate specificity and product specificity of GH68 fructansucrases.

    Science.gov (United States)

    Pijning, Tjaard; Anwar, Munir A; Böger, Markus; Dobruchowska, Justyna M; Leemhuis, Hans; Kralj, Slavko; Dijkhuizen, Lubbert; Dijkstra, Bauke W

    2011-09-09

    Fructansucrases (FSs) catalyze a transfructosylation reaction with sucrose as substrate to produce fructo-oligosaccharides and fructan polymers that contain either β-2,1 glycosidic linkages (inulin) or β-2,6 linkages (levan). Levan-synthesizing FSs (levansucrases) have been most extensively investigated, while detailed information on inulosucrases is limited. Importantly, the molecular basis of the different product specificities of levansucrases and inulosucrases is poorly understood. We have elucidated the three-dimensional structure of a truncated active bacterial GH68 inulosucrase, InuJ of Lactobacillus johnsonii NCC533 (residues 145-708), in its apo form, with a bound substrate (sucrose), and with a transfructosylation product. The sucrose binding pocket and the sucrose binding mode are virtually identical with those of GH68 levansucrases, confirming that both enzyme types use the same fully conserved structural framework for the binding and cleavage of the donor substrate sucrose in the active site. The binding mode of the first transfructosylation product 1-kestose (Fru-β(2-1)-Fru-α(2-1)-Glc, where Fru=fructose and Glc=glucose) in subsites -1 to +2 shows for the first time how inulin-type fructo-oligosaccharide bind in GH68 FS and how an inulin-type linkage can be formed. Surprisingly, observed interactions with the sugar in subsites +1 and +2 are provided by residues that are also present in levansucrases. The binding mode of 1-kestose and the presence of a more distant sucrose binding site suggest that residues beyond the +2 subsite, in particular residues from the nonconserved 1B-1C loop, determine product linkage type specificity in GH68 FSs. Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. Industrial production of insulators using isostatic compaction method

    Energy Technology Data Exchange (ETDEWEB)

    Drugoveiko, O.P.; Ermolaeva, L.V.; Koren' , M.G.; Kreimer, B.D.; Panichev, G.I.; Ponomarev, A.P.; Rutkovskii, V.N.

    1985-07-01

    The process of shaping ceramic products from powders using isostatic compaction method is finding increasing industrial application. The production of electrical-engineering porcelain using isostatic compaction method is, according to the authors, a promising direction since this method permits one to obtain large and complex shaped products having uniform density distribution. The authors introduce an automatic isostatic compaction line at the ''Proletarii'' Factory for the production of the IOS-110-20000UKhL, T1 type insulators having the described dimensions. According to the technological process developed at the ''Elektrokeramika'' Production Complex, insulators were manufactured on the isostatic compaction line from the G-33 mass. Presspowder having a moisture content of 0.3-0.6% and a particle size of 90-160 micrometers was obtained in a spray dryer using disk spraying. The authors studied saturability by moisture of the powder obtained.

  8. Crop and soil specific N and P efficiency and productivity in Finland

    Directory of Open Access Journals (Sweden)

    S. BÄCKMAN

    2008-12-01

    Full Text Available This paper estimates a stochastic production frontier based on experimental data of cereals production in Finland over the period 1977-1994. The estimates of the production frontier are used to analyze nitrogen and phosphorous productivity and efficiency differences between soils and crops. For this input specific efficiencies are calculated. The results can be used to recognize relations between fertilizer management and soil types as well as to learn where certain soil types and crop combinations require special attention to fertilization strategy. The combination of inputs as designed by the experiment shows significant inefficiencies for both N and P. The measures of mineral productivity and efficiency indicate that clay is the most mineral efficient and productive soil while silt and organic soils are the least efficient and productive soils. Furthermore, a positive correlation is found between mineral productivity and efficiency. The results indicate that substantial technical efficiency differences between different experiments prevail.;

  9. Method s for Measuring Productivity in Libraries and Information Centres

    OpenAIRE

    Mohammad Alaaei

    2009-01-01

      Within Information centers, productivity is the result of optimal and effective use of information resources, service quality improvement, increased user satisfaction, pleasantness of working environment, increased motivation and enthusiasm of staff to work better. All contribute to the growth and development of information centers. Thus these centers would need to be familiar with methods employed in productivity measurement. Productivity is one of the criteria for evaluating system perfor...

  10. Comparative Analysis of Hydrogen Production Methods with Nuclear Reactors

    International Nuclear Information System (INIS)

    Morozov, Andrey

    2008-01-01

    Hydrogen is highly effective and ecologically clean fuel. It can be produced by a variety of methods. Presently the most common are through electrolysis of water and through the steam reforming of natural gas. It is evident that the leading method for the future production of hydrogen is nuclear energy. Several types of reactors are being considered for hydrogen production, and several methods exist to produce hydrogen, including thermochemical cycles and high-temperature electrolysis. In the article the comparative analysis of various hydrogen production methods is submitted. It is considered the possibility of hydrogen production with the nuclear reactors and is proposed implementation of research program in this field at the IPPE sodium-potassium eutectic cooling high temperature experimental facility (VTS rig). (authors)

  11. pp production cross sections and the constraint method

    International Nuclear Information System (INIS)

    Anjos, J.C.; Santoro, A.F.S.; Souza, M.H.G.

    1983-01-01

    A method of constructing production cross sections that satisfy the constraints represented by the first few moments is shown to give an excellent account of the data when applied to the high energy pp production cross section ν sub(n) (s) plotted as functions of n. (Author) [pt

  12. Methods Used for Teaching Psychomotor Skills in Crop Production.

    Science.gov (United States)

    Osborne, Edward W.

    1988-01-01

    The purpose of this study was to determine the degree of psychomotor skill instruction in crop production provided by agricultural production teachers in Illinois and the methods used for this teaching. Responses from 79 of 100 teachers indicated that most do not have students observe or practice a procedure for skill improvement. More experienced…

  13. Effects of setting creative goals of different specificity on judged creativity of the product

    OpenAIRE

    Čorko, Irena; Vranić, Andrea

    2005-01-01

    The study examined the effect of setting creative goals of different specificity on judged creativity of the product. Female psychology students (N=47) were divided in 3 groups. Experimental task was to make a collage. Groups differed in the level of specificity of the given goal. Collages were judged by 11 judges using the consensual assessment technique. Factor analysis of these judgments confirmed 2 orthogonal factors: creativity and technical goodness. Results show that setting a specific...

  14. Development of redesign method of production system based on QFD

    Science.gov (United States)

    Kondoh, Shinsuke; Umeda, Yasusi; Togawa, Hisashi

    In order to catch up with rapidly changing market environment, rapid and flexible redesign of production system is quite important. For effective and rapid redesign of production system, a redesign support system is eagerly needed. To this end, this paper proposes a redesign method of production system based on Quality Function Deployment (QFD). This method represents a designer's intention in the form of QFD, collects experts' knowledge as “Production Method (PM) modules,” and formulates redesign guidelines as seven redesign operations so as to support a designer to find out improvement ideas in a systematical manner. This paper also illustrates a redesign support tool of a production system we have developed based on this method, and demonstrates its feasibility with a practical example of a production system of a contact probe. A result from this example shows that comparable cost reduction to those of veteran designers can be achieved by a novice designer. From this result, we conclude our redesign method is effective and feasible for supporting redesign of a production system.

  15. Cell foundry with high product specificity and catalytic activity for 21-deoxycortisol biotransformation.

    Science.gov (United States)

    Xiong, Shuting; Wang, Ying; Yao, Mingdong; Liu, Hong; Zhou, Xiao; Xiao, Wenhai; Yuan, Yingjin

    2017-06-13

    biocatalyst converting 17-OHP to 21-DF. Through the strategies in terms of (1) screening enzymes (with N-terminal mutagenesis) sources, (2) optimizing substrate concentration, and most importantly (3) rational design novel mutants aided by structural analysis, the 21-DF transformation rate was stepwise improved by 19.5-fold along with 4.67-fold increase on the product/byproduct ratio. Eventually, the highest 21-DF reported production was achieved in shake-flask after 38-h biotransformation. This study highlighted above described methods to obtain a high efficient and specific biocatalyst for the desired biotransformation.

  16. Increase in the specific radioactivity of tritium-labeled compounds obtained by tritium thermal activation method

    International Nuclear Information System (INIS)

    Badun, G.A.; Chernysheva, M.G.; Ksenofontov, A.L.

    2012-01-01

    A method of tritium introduction into different types of organic molecules that is based on the interaction of atomic tritium with solid organic target is described. Tritium atoms are formed on the hot W-wire, which is heated by the electric current. Such an approach is called 'tritium thermal activation method'. Here we summarize the results of labeling globular proteins (lysozyme, human and bovine serum albumins); derivatives of pantothenic acid and amino acids; ionic surfactants (sodium dodecylsulfate and alkyltrimethylammonium bromides) and nonionic high-molecular weight surfactants - pluronics. For the first time it is observed that if the target-compound is fixed and its radicals are stable the specific radioactivity of the labeled product can be drastically increased (up to 400 times) when the target temperature is ca. 295 K compared with the results obtained at 77 K. The influence of labeling parameters as tritium gas pressure, exposure time and W-wire temperature was tested for each target temperature that results in the optimum labeling conditions with high specific radioactivity and chemical yield of the resulting compound. (orig.)

  17. IMPLEMENTATION OF CONTROL CARDS AND SUPPORTING METHOD IN PRODUCTION ENGINEERING

    Directory of Open Access Journals (Sweden)

    Anna WOLNOWSKA

    2012-10-01

    Full Text Available In the article there were presented chosen method associated with statistical control of production processes. Mainly focused on control cards and Pareto‐Lorenz analysis. Showed method were implemented to analysis production process stability of hearing aids in X company (the brand name don’t give because date of production is secret. Researches were made few months after new assembly lines starts‐up. Main aim of researches was defects types identification occurred in production process and determine the scale of effect. Finally received results were satisfactory, i.e. despite of occurred errors, control cards analysis showed that production process of BTE‐type (Behind‐The‐Ear hearing aids was stable.

  18. The New Product Watch: Successes and Challenges of Crowdsourcing as a Method of Surveillance.

    Science.gov (United States)

    Nyman, Amy L; Biener, Lois

    2016-01-01

    New smokeless tobacco (eg, snus and dissolvable tobacco products) and nontobacco nicotine products (eg, e-cigarettes) have emerged in recent years amid widespread speculation about locations of test marketing, toxic constituents, and consumer targeting. The New Product Watch was a pilot online monitoring system aimed at filling these information gaps by using a form of crowdsourcing: recruiting volunteers to visit local retailers and report their findings. With very little funding, the New Product Watch gathered county-specific data on new product availability in 19 states as well as trend data on product marketing and demand, and completed 2 rounds of product purchases and subsequent toxic constituent analyses. Data were collected over a 2-year period, between 2009 and 2011. Despite the successes, we found that this small-scale, volunteer effort was not a sustainable method for ensuring continuous, systematic surveillance of new product availability, marketing, and toxicity.

  19. Detection and Characterization of Reactive Oxygen and Nitrogen Species in Biological Systems by Monitoring Species-Specific Products.

    Science.gov (United States)

    Hardy, Micael; Zielonka, Jacek; Karoui, Hakim; Sikora, Adam; Michalski, Radosław; Podsiadły, Radosław; Lopez, Marcos; Vasquez-Vivar, Jeannette; Kalyanaraman, Balaraman; Ouari, Olivier

    2018-05-20

    Since the discovery of the superoxide dismutase enzyme, the generation and fate of short-lived oxidizing, nitrosating, nitrating, and halogenating species in biological systems has been of great interest. Despite the significance of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in numerous diseases and intracellular signaling, the rigorous detection of ROS and RNS has remained a challenge. Recent Advances: Chemical characterization of the reactions of selected ROS and RNS with electron paramagnetic resonance (EPR) spin traps and fluorescent probes led to the establishment of species-specific products, which can be used for specific detection of several forms of ROS and RNS in cell-free systems and in cultured cells in vitro and in animals in vivo. Profiling oxidation products from the ROS and RNS probes provides a rigorous method for detection of those species in biological systems. Formation and detection of species-specific products from the probes enables accurate characterization of the oxidative environment in cells. Measurement of the total signal (fluorescence, chemiluminescence, etc.) intensity does not allow for identification of the ROS/RNS formed. It is critical to identify the products formed by using chromatographic or other rigorous techniques. Product analyses should be accompanied by monitoring of the intracellular probe level, another factor controlling the yield of the product(s) formed. More work is required to characterize the chemical reactivity of the ROS/RNS probes, and to develop new probes/detection approaches enabling real-time, selective monitoring of the specific products formed from the probes. Antioxid. Redox Signal. 28, 1416-1432.

  20. Biodiesel production methods of rubber seed oil: a review

    Science.gov (United States)

    Ulfah, M.; Mulyazmi; Burmawi; Praputri, E.; Sundari, E.; Firdaus

    2018-03-01

    The utilization of rubber seed as raw material of biodiesel production is seen highly potential in Indonesia. The availability of rubber seeds in Indonesia is estimated about 5 million tons per annum, which can yield rubber seed oil about 2 million tons per year. Due to the demand of edible oils as a food source is tremendous and the edible oil feedstock costs are far expensive to be used as fuel, production of biodiesel from non-edible oils such as rubber seed is an effective way to overcome all the associated problems with edible oils. Various methods for producing biodiesel from rubber seed oil have been reported. This paper introduces an optimum condition of biodiesel production methods from rubber seed oil. This article was written to be a reference in the selection of methods and the further development of biodiesel production from rubber seed oil. Biodiesel production methods for rubber seed oils has been developed by means of homogeneous catalysts, heterogeneous catalysts, supercritical method, ultrasound, in-situ and enzymatic processes. Production of biodiesel from rubber seed oil using clinker loaded sodium methoxide as catalyst is very interesting to be studied and developed further.

  1. Application of Specific Features of Industrial Products when Forming and Developing Brands of Industrial Enterprises

    Directory of Open Access Journals (Sweden)

    Yatsentiuk Stanislav V.

    2014-03-01

    Full Text Available The article analyses and structures approaches and principles of formulation of industrial products. It offers classification of goods and markets of industrial products by their characteristics and participants. It identifies main participants that make decisions at B2C and B2B markets and characterises their specific features and motivation when making decisions on purchase of products of industrial enterprises. It studies and analyses indicators of development of domestic markets of consumer goods and market of industrial products and dynamics of development of their relation in retrospective view.

  2. Overview on recent developments: alternative isotope production methods in Canada

    International Nuclear Information System (INIS)

    Huynh, K.

    2012-01-01

    The purpose of this paper is to provide an update on the Government of Canada's programs in alternative isotope production methods for securing supply of technetium 99m for Canadians. The supply disruptions of isotopes in 2007 and 2009/2010 caused by unplanned outages at AECL's National Research Universal (NRU) reactor highlighted the fragility of the supply chain that delivers medical isotopes, specifically Technetium 99m (Tc99m) to patients in Canada and globally. Tc99m, which is derived from its parent, molybdenum99 (Mo99) is the most widely used medical isotope for imaging, and accounts for 80 percent of nuclear medicine diagnostic procedures. Prior to the outage, nearly all the Mo99 produced for the world market came from five aging government owned research reactors in Canada, France, the Netherlands, Belgium and South Africa. The NRU, the largest of these, produced about 30 to 40 percent of the world supply of isotopes prior to 2009 - since its return to service in 2010, its world market share is estimated at 15 to 20%.

  3. Analysis of alternative methods and price politic of icewine production

    Directory of Open Access Journals (Sweden)

    V. Ostapenko

    2017-06-01

    Full Text Available The artificial methods of must concentration were discussed in current study: the microwave vacuum dehydration, reverse osmosis and cryoextraction. The main factor of using of alternative ways is deficiently low temperatures in winter period that are necessary for freezing grapes on vine according to the classical technology. The benefits and disadvantages of using of non-classic processes to obtain sweet musts were shown. The physical, chemical and sensory characteristics of wine made from grapes previously frozen by alternative and natural ways were analyzed. Indicators influencing on price of icewines and dessert wines bottle including agricultural climatic, technological and marketing factors were determined.  Detailed indicators highlight specificity of used technology and represent consumer preferences. Producers of winemaking regions of Argentina, New Zealand, Israel, Ukraine and Australia adhere to provisions that are inconsistent with the standards of Canada and the European countries regarding the icewine output. These instruments determine the processing of grapes and parameters reflect on parameters of the finished product.

  4. Gradient-based methods for production optimization of oil reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Suwartadi, Eka

    2012-07-01

    Production optimization for water flooding in the secondary phase of oil recovery is the main topic in this thesis. The emphasis has been on numerical optimization algorithms, tested on case examples using simple hypothetical oil reservoirs. Gradientbased optimization, which utilizes adjoint-based gradient computation, is used to solve the optimization problems. The first contribution of this thesis is to address output constraint problems. These kinds of constraints are natural in production optimization. Limiting total water production and water cut at producer wells are examples of such constraints. To maintain the feasibility of an optimization solution, a Lagrangian barrier method is proposed to handle the output constraints. This method incorporates the output constraints into the objective function, thus avoiding additional computations for the constraints gradient (Jacobian) which may be detrimental to the efficiency of the adjoint method. The second contribution is the study of the use of second-order adjoint-gradient information for production optimization. In order to speedup convergence rate in the optimization, one usually uses quasi-Newton approaches such as BFGS and SR1 methods. These methods compute an approximation of the inverse of the Hessian matrix given the first-order gradient from the adjoint method. The methods may not give significant speedup if the Hessian is ill-conditioned. We have developed and implemented the Hessian matrix computation using the adjoint method. Due to high computational cost of the Newton method itself, we instead compute the Hessian-timesvector product which is used in a conjugate gradient algorithm. Finally, the last contribution of this thesis is on surrogate optimization for water flooding in the presence of the output constraints. Two kinds of model order reduction techniques are applied to build surrogate models. These are proper orthogonal decomposition (POD) and the discrete empirical interpolation method (DEIM

  5. Determination of Aspergillus pathogens in agricultural products by a specific nanobody-polyclonal antibody sandwich ELISA.

    Science.gov (United States)

    Wang, Ting; Li, Peiwu; Zhang, Qi; Zhang, Wen; Zhang, Zhaowei; Wang, Tong; He, Ting

    2017-06-28

    Aspergillus and its poisonous mycotoxins are distributed worldwide throughout the environment and are of particular interest in agriculture and food safety. In order to develop a specific method for rapid detection of Aspergillus flavus to forecast diseases and control aflatoxins, a nanobody, PO8-VHH, highly reactive to A. flavus was isolated from an immunized alpaca nanobody library by phage display. The nanobody was verified to bind to the components of extracellular and intracellular antigen from both A. flavus and A. parasiticus. To construct a sandwich format immunoassay, polyclonal antibodies against Aspergillus were raised with rabbits. Finally, a highly selective nanobody-polyclonal antibody sandwich enzyme-linked immunosorbent assay was optimized and developed. The results revealed that the detection limits of the two fungi were as low as 1 μg mL -1 , and that it is able to detect fungal concentrations below to 2 μg mg -1 of peanut and maize grains in both artificially and naturally contaminated samples. Therefore, we here provided a rapid and simple method for monitoring Aspergillus spp. contamination in agricultural products.

  6. Methods for production of proteins in host cells

    Science.gov (United States)

    Donnelly, Mark; Joachimiak, Andrzej

    2004-01-13

    The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.

  7. Radiation methods for demercaptanization and desulfurization of oil products

    International Nuclear Information System (INIS)

    Zaykina, R.F.; Zaykin, Yu.A.; Mamonova, T.B.; Nadirov, N.K.

    2002-01-01

    A two-stage method for the desulfurization of oil is presented. The first stage strongly oxidizes sulfuric material to do away with its chemical aggressiveness and promote its removal. Desulfurization of the overall product is reached at the second stage by means of conventional methods

  8. Radiation methods for demercaptanization and desulfurization of oil products

    Science.gov (United States)

    Zaykina, R. F.; Zaykin, Yu. A.; Mamonova, T. B.; Nadirov, N. K.

    2002-03-01

    A two-stage method for the desulfurization of oil is presented. The first stage strongly oxidizes sulfuric material to do away with its chemical aggressiveness and promote its removal. Desulfurization of the overall product is reached at the second stage by means of conventional methods.

  9. Prostate-Specific Natural Health Products (Dietary Supplements) Radiosensitize Normal Prostate Cells

    International Nuclear Information System (INIS)

    Hasan, Yasmin; Schoenherr, Diane; Martinez, Alvaro A.; Wilson, George D.; Marples, Brian

    2010-01-01

    Purpose: Prostate-specific health products (dietary supplements) are taken by cancer patients to alleviate the symptoms linked with poor prostate health. However, the effect of these agents on evidence-based radiotherapy practice is poorly understood. The present study aimed to determine whether dietary supplements radiosensitized normal prostate or prostate cancer cell lines. Methods and Materials: Three well-known prostate-specific dietary supplements were purchased from commercial sources available to patients (Trinovin, Provelex, and Prostate Rx). The cells used in the study included normal prostate lines (RWPE-1 and PWR-1E), prostate tumor lines (PC3, DU145, and LNCaP), and a normal nonprostate line (HaCaT). Supplement toxicity was assessed using cell proliferation assays [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and cellular radiosensitivity using conventional clonogenic assays (0.5-4Gy). Cell cycle kinetics were assessed using the bromodeoxyuridine/propidium iodide pulse-labeling technique, apoptosis by scoring caspase-3 activation, and DNA repair by assessing γH2AX. Results: The cell growth and radiosensitivity of the malignant PC3, DU145, and LNcaP cells were not affected by any of the dietary prostate supplements (Provelex [2μg/mL], Trinovin [10μg/mL], and Prostate Rx [50 μg/mL]). However, both Trinovin (10μg/mL) and Prostate Rx (6μg/mL) inhibited the growth rate of the normal prostate cell lines. Prostate Rx increased cellular radiosensitivity of RWPE-1 cells through the inhibition of DNA repair. Conclusion: The use of prostate-specific dietary supplements should be discouraged during radiotherapy owing to the preferential radiosensitization of normal prostate cells.

  10. Improved Methods for Production Manufacturing Processes in Environmentally Benign Manufacturing

    Directory of Open Access Journals (Sweden)

    Yan-Yan Wang

    2011-09-01

    Full Text Available How to design a production process with low carbon emissions and low environmental impact as well as high manufacturing performance is a key factor in the success of low-carbon production. It is important to address concerns about climate change for the large carbon emission source manufacturing industries because of their high energy consumption and environmental impact during the manufacturing stage of the production life cycle. In this paper, methodology for determining a production process is developed. This methodology integrates process determination from three different levels: new production processing, selected production processing and batch production processing. This approach is taken within a manufacturing enterprise based on prior research. The methodology is aimed at providing decision support for implementing Environmentally Benign Manufacturing (EBM and low-carbon production to improve the environmental performance of the manufacturing industry. At the first level, a decision-making model for new production processes based on the Genetic Simulated Annealing Algorithm (GSAA is presented. The decision-making model considers not only the traditional factors, such as time, quality and cost, but also energy and resource consumption and environmental impact, which are different from the traditional methods. At the second level, a methodology is developed based on an IPO (Input-Process-Output model that integrates assessments of resource consumption and environmental impact in terms of a materials balance principle for batch production processes. At the third level, based on the above two levels, a method for determining production processes that focus on low-carbon production is developed based on case-based reasoning, expert systems and feature technology for designing the process flow of a new component. Through the above three levels, a method for determining the production process to identify, quantify, assess, and optimize the

  11. METHOD FOR THE MEASUREMENT OF SITE-SPECIFIC TAUTOMERIC AND ZWITTERIONIC MICROSPECIES EQUILIBRIUM CONSTANTS

    Science.gov (United States)

    We describe a method for the individual measurement of simultaneously occurring, unimolecular, site-specific “microequilibrium” constants as in, for example, prototropic tautomerism and zwitterionic equilibria. Our method represents an elaboration of that of Nygren et al. (Anal. ...

  12. A Normative Definition Method for Origin Food Products

    NARCIS (Netherlands)

    Meulen, van der H.S.

    2007-01-01

    The aim of this article is to define a methodological tool to evaluate the contribution of various factors to the connectedness of food products to their places of origin, i.e. to the specific market potential that arises from that link. Most of the examples given here are drawn from the Dutch

  13. Method for reducing ammonium and lactate production in cho cells

    DEFF Research Database (Denmark)

    2018-01-01

    The present invention relates to modified producer cells for improved production of therapeutic proteins. Specifically, the inventors have found that removing genes involved in amino acid catabolism in Chinese Hamster Ovary (CHO) cells improves the cell growth and viability and likely also...

  14. Malmquist Productivity Index by Extended VIKOR Method Using Interval Numbers

    OpenAIRE

    Fallah, Mohammad; Mohajeri, Amir; Najafi, Esmaeil

    2013-01-01

    The VIKOR method was developed for multicriteria optimization of complex systems. It determines the compromise ranking list and the compromise solution obtained with the given weights. This method focuses on ranking and selecting from a set of alternatives in the presence of conflicting criteria. Here, the VIKOR method is used for two times $t$ and $t+1$ . In order to calculate the progress or regression via Malmquist productivity index, the positive and negative ideals at ...

  15. Production of radiohalogens and [11C]-methane at high specific activity

    Science.gov (United States)

    Nye, Jonathon Andrew

    2005-07-01

    The halogens, occupying Group VII of the periodic table, play an important role in the biochemical processes underlying health and disease. A variety of positron emitters covering a broad range of half-lives permit the imaging of the body's physiochemical behavior using PET. Neutron deficient isotopes of the halogen group can be produced by (p,n) reactions from enriched targets with low energy (production process insures their role in the clinical/research environment, labeling H215 O, 13NH3, CH3-compounds and 18F-FDG. Halogens with half-lives longer than 18F have been avoided due to a combination of several factors, such as complexity of the target systems, expense of the enriched substrate, low reaction yields, and extensive post-processing to reclaim the target material. PET research over the last decade has forced a match between drug development and emerging small animal instrumentation, shifting focus to agents labeled with high specific activity 11CH3I and the long-lived radiohalogens, 76Br and 124I. A steady local supply of 18F-fluoride, 11C-methane, 76B-bromide, and 124I-iodide is essential to seize today's research opportunities or for limited distribution outside of our local area. To keep pace, new targetry developments are implemented to reliably produce these isotopes on a batch basis. The research presented details improvements on existing production methods for 18F-fluoride intended for nucleophilic substitution and high specific activity 11C-methane (→CH3I) for the N-methylation of a half-dozen neuroligands. A significant effort is placed on the novel use of low energy cyclotrons for the production of 76Br and 124I involved in labeling antibody and protein agents. Performance of these new designs and the success of the solid targetry development will be described. The ten-fold scale-up in yields at end-of-bombardment promises new hope for the synthesis of PET tracers, previously limited by access to the radio-halogen precursors.

  16. Carbapenem inactivation: a very affordable and highly specific method for phenotypic detection of carbapenemase-producing Pseudomonas aeruginosa isolates compared with other methods.

    Science.gov (United States)

    Akhi, Mohammad Taghi; Khalili, Younes; Ghotaslou, Reza; Kafil, Hossein Samadi; Yousefi, Saber; Nagili, Behroz; Goli, Hamid Reza

    2017-06-01

    This investigation was undertaken to compare phenotypic and molecular methods for detection of carbapenemase-producing Pseudomonas aeruginosa. A total of 245 non-duplicated isolates of P. aeruginosa were collected from hospitalized patients. Disc diffusion method was used to identify carbapenem-resistant bacteria. Three phenotypic methods, including Modified Hodge Test (MHT), Modified Carba NP (MCNP) test and Carbapenem Inactivation Method (CIM) were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was used to detect carbapenemase encoding genes. Of 245 P. aeruginosa isolates investigated, 121 isolates were carbapenem-resistant. Among carbapenem-resistant isolates, 40, 39 and 35 isolates exhibited positive results using MHT, MCNP test and CIM, respectively. PCR indicated the presence of carbapenemase genes in 35 of carbapenem-resistant isolates. MHT showed low sensitivity and specificity for carbapenemase detection among P. aeruginosa isolates in comparison to PCR. CIM was most affordable and highly specific than MCNP test compared with the molecular method.

  17. Specific Methods of Information Security for Nuclear Materials Control and Accounting Automate Systems

    Directory of Open Access Journals (Sweden)

    Konstantin Vyacheslavovich Ivanov

    2013-02-01

    Full Text Available The paper is devoted to specific methods of information security for nuclear materials control and accounting automate systems which is not required of OS and DBMS certifications and allowed to programs modification for clients specific without defenses modification. System ACCORD-2005 demonstrates the realization of this method.

  18. Overview of the European Medicines Agency's Development of Product-Specific Bioequivalence Guidelines.

    Science.gov (United States)

    Sullivan, Jane O'; Blake, Kevin; Berntgen, Michael; Salmonson, Tomas; Welink, Jan

    2017-12-05

    The European Medicines Agency's (EMA) product-specific bioequivalence guidelines outline harmonized regulatory requirements for studies to demonstrate bioequivalence for products that may have particular needs due to their pharmacokinetics, in addition to those outlined in general guidance. As such they are potentially very useful to the pharmaceutical industry in the development of generic medicinal products and to regulatory authorities for harmonized decision-making. Since their introduction in 2013, EMA product-specific bioequivalence guidelines continue to increase in number, and as of June 2017, encompass a number of different pharmacotherapeutic groups and pharmaceutical forms. This article further elucidates the processes involved for stakeholders and reviews the Agency's experience with the development of these guidelines, including the scientific issues witnessed with their advancement. A comparison with the United States Food and Drug Administration approach to similar guidelines is also provided. © 2017 The Authors Clinical Pharmacology & Therapeutics published by Wiley Periodicals, Inc. on behalf of American Society for Clinical Pharmacology and Therapeutics.

  19. Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures.

    Science.gov (United States)

    Du, Zhimei; Treiber, David; McCarter, John D; Fomina-Yadlin, Dina; Saleem, Ramsey A; McCoy, Rebecca E; Zhang, Yuling; Tharmalingam, Tharmala; Leith, Matthew; Follstad, Brian D; Dell, Brad; Grisim, Brent; Zupke, Craig; Heath, Carole; Morris, Arvia E; Reddy, Pranhitha

    2015-01-01

    The continued need to improve therapeutic recombinant protein productivity has led to ongoing assessment of appropriate strategies in the biopharmaceutical industry to establish robust processes with optimized critical variables, that is, viable cell density (VCD) and specific productivity (product per cell, qP). Even though high VCD is a positive factor for titer, uncontrolled proliferation beyond a certain cell mass is also undesirable. To enable efficient process development to achieve consistent and predictable growth arrest while maintaining VCD, as well as improving qP, without negative impacts on product quality from clone to clone, we identified an approach that directly targets the cell cycle G1-checkpoint by selectively inhibiting the function of cyclin dependent kinases (CDK) 4/6 with a small molecule compound. Results from studies on multiple recombinant Chinese hamster ovary (CHO) cell lines demonstrate that the selective inhibitor can mediate a complete and sustained G0/G1 arrest without impacting G2/M phase. Cell proliferation is consistently and rapidly controlled in all recombinant cell lines at one concentration of this inhibitor throughout the production processes with specific productivities increased up to 110 pg/cell/day. Additionally, the product quality attributes of the mAb, with regard to high molecular weight (HMW) and glycan profile, are not negatively impacted. In fact, high mannose is decreased after treatment, which is in contrast to other established growth control methods such as reducing culture temperature. Microarray analysis showed major differences in expression of regulatory genes of the glycosylation and cell cycle signaling pathways between these different growth control methods. Overall, our observations showed that cell cycle arrest by directly targeting CDK4/6 using selective inhibitor compound can be utilized consistently and rapidly to optimize process parameters, such as cell growth, qP, and glycosylation profile in

  20. Method of producing gaseous products using a downflow reactor

    Science.gov (United States)

    Cortright, Randy D; Rozmiarek, Robert T; Hornemann, Charles C

    2014-09-16

    Reactor systems and methods are provided for the catalytic conversion of liquid feedstocks to synthesis gases and other noncondensable gaseous products. The reactor systems include a heat exchange reactor configured to allow the liquid feedstock and gas product to flow concurrently in a downflow direction. The reactor systems and methods are particularly useful for producing hydrogen and light hydrocarbons from biomass-derived oxygenated hydrocarbons using aqueous phase reforming. The generated gases may find used as a fuel source for energy generation via PEM fuel cells, solid-oxide fuel cells, internal combustion engines, or gas turbine gensets, or used in other chemical processes to produce additional products. The gaseous products may also be collected for later use or distribution.

  1. Improvement of organization and audit methods of production reserves at the enterprise

    Directory of Open Access Journals (Sweden)

    M.I. Skrypnyk

    2016-12-01

    Full Text Available The current research deals with the scientific substantiation of theoretical positions and development of practical recommendations for improving the organization and audit methodology of production reserves at an enterprise. The authors apply the methods of specification. Testing, analysis, synthesis, generalization are the basis for the improvement of the organization and audit methodology of production reserves at an enterprise. The researchers formulate the goals, objectives, information sources and basic methods used in the audit of production reserves. The article examines the main stages of the audit of production reserves and the methodology of its implementation. The algorithm of auditing of production reserves is defined. The paper examines the basic working papers of the auditor for auditing of production reserves such as the test on internal control of production reserves, the audit plan of production reserves, the audit program of production reserves, the comparative statement of inventory resultsof capital assets. These documents, in their turn, will increase the level of control and improve the quality of audit operations with production reserves. The article determines the common errors that occur during operations with production reserves tand you can avoid the errors using the developed plan, program and audit working papers used to conduct the audit of production reserves.

  2. Validation of method in instrumental NAA for food products sample

    International Nuclear Information System (INIS)

    Alfian; Siti Suprapti; Setyo Purwanto

    2010-01-01

    NAA is a method of testing that has not been standardized. To affirm and confirm that this method is valid. it must be done validation of the method with various sample standard reference materials. In this work. the validation is carried for food product samples using NIST SRM 1567a (wheat flour) and NIST SRM 1568a (rice flour). The results show that the validation method for testing nine elements (Al, K, Mg, Mn, Na, Ca, Fe, Se and Zn) in SRM 1567a and eight elements (Al, K, Mg, Mn, Na, Ca, Se and Zn ) in SRM 1568a pass the test of accuracy and precision. It can be conclude that this method has power to give valid result in determination element of the food products samples. (author)

  3. Interannual Variation in Phytoplankton Class-Specific Primary Production at a Global Scale

    Science.gov (United States)

    Rousseaux, Cecile Severine; Gregg, Watson W.

    2014-01-01

    We used the NASA Ocean Biogeochemical Model (NOBM) combined with remote sensing data via assimilation to evaluate the contribution of 4 phytoplankton groups to the total primary production. First we assessed the contribution of each phytoplankton groups to the total primary production at a global scale for the period 1998-2011. Globally, diatoms were the group that contributed the most to the total phytoplankton production (50, the equivalent of 20 PgC y-1. Coccolithophores and chlorophytes each contributed to 20 (7 PgC y-1 of the total primary production and cyanobacteria represented about 10 (4 PgC y(sub-1) of the total primary production. Primary production by diatoms was highest in high latitude (45) and in major upwelling systems (Equatorial Pacific and Benguela system). We then assessed interannual variability of this group-specific primary production over the period 1998-2011. Globally the annual relative contribution of each phytoplankton groups to the total primary production varied by maximum 4 (1-2 PgC y-1. We assessed the effects of climate variability on the class-specific primary production using global (i.e. Multivariate El Nio Index, MEI) and regional climate indices (e.g. Southern Annular Mode (SAM), Pacific Decadal Oscillation (PDO) and North Atlantic Oscillation (NAO)). Most interannual variability occurred in the Equatorial Pacific and was associated with climate variability as indicated by significant correlation (p 0.05) between the MEI and the class-specific primary production from all groups except coccolithophores. In the Atlantic, climate variability as indicated by NAO was significantly correlated to the primary production of 2 out of the 4 groups in the North Central Atlantic (diatomscyanobacteria) and in the North Atlantic (chlorophytes and coccolithophores). We found that climate variability as indicated by SAM had only a limited effect on the class-specific primary production in the Southern Ocean. These results provide a modeling and

  4. Novel Method of Lactic Acid Production by Electrodialysis Fermentation

    OpenAIRE

    Hongo, Motoyoshi; Nomura, Yoshiyuki; Iwahara, Masayoshi

    1986-01-01

    In lactic acid fermentation by Lactobacillus delbrueckii, the produced lactic acid affected the lactic acid productivity. Therefore, for the purpose of alleviating this inhibitory effect, an electrodialysis fermentation method which can continuously remove produced lactic acid from the fermentation broth was applied to this fermentation process. As a result, the continuation of fermentation activity was obtained, and the productivity was three times higher than in non-pH-controlled fermentati...

  5. Methods and systems for the production of hydrogen

    Science.gov (United States)

    Oh, Chang H [Idaho Falls, ID; Kim, Eung S [Ammon, ID; Sherman, Steven R [Augusta, GA

    2012-03-13

    Methods and systems are disclosed for the production of hydrogen and the use of high-temperature heat sources in energy conversion. In one embodiment, a primary loop may include a nuclear reactor utilizing a molten salt or helium as a coolant. The nuclear reactor may provide heat energy to a power generation loop for production of electrical energy. For example, a supercritical carbon dioxide fluid may be heated by the nuclear reactor via the molten salt and then expanded in a turbine to drive a generator. An intermediate heat exchange loop may also be thermally coupled with the primary loop and provide heat energy to one or more hydrogen production facilities. A portion of the hydrogen produced by the hydrogen production facility may be diverted to a combustor to elevate the temperature of water being split into hydrogen and oxygen by the hydrogen production facility.

  6. Grasping devices and methods in automated production processes

    DEFF Research Database (Denmark)

    Fantoni, Gualtiero; Santochi, Marco; Dini, Gino

    2014-01-01

    assembly to disassembly, from aerospace to food industry, from textile to logistics) are discussed. Finally, the most recent research is reviewed in order to introduce the new trends in grasping. They provide an outlook on the future of both grippers and robotic hands in automated production processes. (C......In automated production processes grasping devices and methods play a crucial role in the handling of many parts, components and products. This keynote paper starts with a classification of grasping phases, describes how different principles are adopted at different scales in different applications...

  7. An update on neurotoxin products and administration methods.

    Science.gov (United States)

    Lanoue, Julien; Dong, Joanna; Do, Timothy; Goldenberg, Gary

    2016-09-01

    Since onabotulinumtoxinA for nonsurgical aesthetic enhancement of glabellar lines was initially reported, the popularity of botulinum neurotoxin (BoNT) products among both clinicians and consumers has rapidly grown, and we have seen several additional BoNT formulations enter the market. As the demand for minimally invasive cosmetic procedures continues to increase, we will see the introduction of additional formulations of BoNT products as well as new delivery devices and administration techniques. In this article, we provide a brief update on current and upcoming BoNT products and also review the literature on novel administration methods based on recently published studies.

  8. User Experience Evaluation Methods in Product Development (UXEM'09)

    Science.gov (United States)

    Roto, Virpi; Väänänen-Vainio-Mattila, Kaisa; Law, Effie; Vermeeren, Arnold

    High quality user experience (UX) has become a central competitive factor of product development in mature consumer markets [1]. Although the term UX originated from industry and is a widely used term also in academia, the tools for managing UX in product development are still inadequate. A prerequisite for designing delightful UX in an industrial setting is to understand both the requirements tied to the pragmatic level of functionality and interaction and the requirements pertaining to the hedonic level of personal human needs, which motivate product use [2]. Understanding these requirements helps managers set UX targets for product development. The next phase in a good user-centered design process is to iteratively design and evaluate prototypes [3]. Evaluation is critical for systematically improving UX. In many approaches to UX, evaluation basically needs to be postponed until the product is fully or at least almost fully functional. However, in an industrial setting, it is very expensive to find the UX failures only at this phase of product development. Thus, product development managers and developers have a strong need to conduct UX evaluation as early as possible, well before all the parts affecting the holistic experience are available. Different types of products require evaluation on different granularity and maturity levels of a prototype. For example, due to its multi-user characteristic, a community service or an enterprise resource planning system requires a broader scope of UX evaluation than a microwave oven or a word processor that is meant for a single user at a time. Before systematic UX evaluation can be taken into practice, practical, lightweight UX evaluation methods suitable for different types of products and different phases of product readiness are needed. A considerable amount of UX research is still about the conceptual frameworks and models for user experience [4]. Besides, applying existing usability evaluation methods (UEMs) without

  9. Specific elements of communication on agri-food products resulting from EU legislation

    Directory of Open Access Journals (Sweden)

    Laura Timiras

    2012-12-01

    Full Text Available In the European Union a significant number of regulations aimed at agricultural and food sector thus creating the legal framework regarding the mode of production, pricing, distribution and communication in relation to agri-food products. Specificity agri-food marketing communication at EU level resulting in most of the measures taken with regard to ensuring consumer protection, food safety and security, some of them, in force in early 2012, referring to the labeling, presentation and advertising of agri-food products, sponsorship and distinctive signs of quality are shown in this paper.

  10. Soil Moisture Active Passive (SMAP) Mission Level 4 Carbon (L4_C) Product Specification Document

    Science.gov (United States)

    Glassy, Joe; Kimball, John S.; Jones, Lucas; Reichle, Rolf H.; Ardizzone, Joseph V.; Kim, Gi-Kong; Lucchesi, Robert A.; Smith, Edmond B.; Weiss, Barry H.

    2015-01-01

    This is the Product Specification Document (PSD) for Level 4 Surface and Root Zone Soil Moisture (L4_SM) data for the Science Data System (SDS) of the Soil Moisture Active Passive (SMAP) project. The L4_SM data product provides estimates of land surface conditions based on the assimilation of SMAP observations into a customized version of the NASA Goddard Earth Observing System, Version 5 (GEOS-5) land data assimilation system (LDAS). This document applies to any standard L4_SM data product generated by the SMAP Project.

  11. Preparing the Production of a New Product in Small and Medium-Sized Enterprises by Using the Method of Projects Management

    Science.gov (United States)

    Bijańska, Jolanta; Wodarski, Krzysztof; Wójcik, Janusz

    2016-06-01

    Efficient and effective preparation the production of new products is important requirement for a functioning and development of small and medium-sized enterprises. One of the methods, which support the fulfilment of this condition is project management. This publication presents the results of considerations, which are aimed at developing a project management model of preparation the production of a new product, adopted to specificity of small and medium-sized enterprises.

  12. Engineering Mathematical Analysis Method for Productivity Rate in Linear Arrangement Serial Structure Automated Flow Assembly Line

    Directory of Open Access Journals (Sweden)

    Tan Chan Sin

    2015-01-01

    Full Text Available Productivity rate (Q or production rate is one of the important indicator criteria for industrial engineer to improve the system and finish good output in production or assembly line. Mathematical and statistical analysis method is required to be applied for productivity rate in industry visual overviews of the failure factors and further improvement within the production line especially for automated flow line since it is complicated. Mathematical model of productivity rate in linear arrangement serial structure automated flow line with different failure rate and bottleneck machining time parameters becomes the basic model for this productivity analysis. This paper presents the engineering mathematical analysis method which is applied in an automotive company which possesses automated flow assembly line in final assembly line to produce motorcycle in Malaysia. DCAS engineering and mathematical analysis method that consists of four stages known as data collection, calculation and comparison, analysis, and sustainable improvement is used to analyze productivity in automated flow assembly line based on particular mathematical model. Variety of failure rate that causes loss of productivity and bottleneck machining time is shown specifically in mathematic figure and presents the sustainable solution for productivity improvement for this final assembly automated flow line.

  13. Comparison of specific production performances by two crystalline silicon PV systems

    Directory of Open Access Journals (Sweden)

    Martin Fajman

    2013-01-01

    Full Text Available A comparison of two independent photovoltaic (PV systems located close to each other on the south of the Czech Moravian Highland was accomplished. Due to differences in installation parameters; reference quantities were used to calculate transformed data sets for specific production performances comparison. Differences in monthly and annually daily production were performed by t-test.According to obtained results, it was concluded that annually mean daily productions per 1 kWp of installed capacity and per 1 m2 of active area of the panels are significantly better by single crystal silicon installation in tracking system than by stable installation of a different technology of single crystal silicon. However, comparing this performance per 1 m2 of occupied land by studied power-plants the stable installation performed higher production rates on daily mean basis in majority of months of the year 2010 as well as by annually mean daily production.

  14. Method for Bacterial Growth and Ammonia Production and Effect of Inhibitory Substances in Disposable Absorbent Hygiene Products.

    Science.gov (United States)

    Forsgren-Brusk, Ulla; Yhlen, Birgitta; Blomqvist, Marie; Larsson, Peter

    The purpose of this study was to evaluate a pragmatic laboratory method to provide a technique for developing incontinence products better able to reduce malodor when used in the clinical setting. Bacterial growth and bacterially formed ammonia in disposable absorbent incontinence products was measured by adding synthetic urine inoculated with bacteria to test samples cut from the crotch area of the product. The inhibitory effect's of low pH (4.5 and 4.9) and 3 antimicrobial substances-chlorhexidine, polyhexamethylene biguanide (PHMB), and thymol-at 2 concentrations each, were studied. From the initial inocula of 3.3 log colony-forming units per milliliter (cfu/mL) at baseline, the bacterial growth of the references increased to 5.0 to 6.0 log cfu/mL at 6 hours for Escherichia coli, Proteus mirabilis, and Enterococcus faecalis. At 12 hours there was a further increase to 7.0 to 8.9 log cfu/mL. Adjusting the pH of the superabsorbent in the incontinence product from 6.0 to pH 4.5 and pH 4.9 significantly (P disposable absorbent products to inhibit bacterial growth and ammonia production. This technique, we describe, provides a pragmatic method for assessing the odor-inhibiting capacity of specific incontinence products.

  15. Changeability of consumer preferences concerning the methods of fruit production

    Directory of Open Access Journals (Sweden)

    Eugenia Czernyszewicz

    2009-01-01

    Full Text Available The purpose of the paper was to establish and compare consumer preferences concerning the methods of fruit production (traditional or organic ones in the years 2001, 2003 and 2006 and the relations between the preferences and the socio-economic and demographic features of the consumers. The analysis was conducted on the basis of the data from surveys carried out among the inhabitants of Lublin. Results of those surveys point out that certain features of the consumers such as the sex, incomes and the family type significantly differentiated preferences concerning the method of fruit production. Increased incomes were connected with greater acceptance of the organic method, and their decrease was related to greater frequency of indicating the conventional method. Interest in the method of production, while buying the fruit was significantly higher among men than among women. Declaring the willingness to pay more for organic fruit was also correlated with the consumers’ sex. Besides, in 2006 it was not too strongly related to the incomes of the respondents. In the years 2001 and 2006 changeability of preferences con-cerning the willingness to pay a higher price for organic fruit and no change in the interest in the technology of fruit production while purchasing the fruit were shown.

  16. Method for indirect quantification of CH4 production via H2O production using hydrogenotrophic methanogens

    Directory of Open Access Journals (Sweden)

    Ruth-Sophie eTaubner

    2016-04-01

    Full Text Available ydrogenotrophic methanogens are an intriguing group of microorganisms from the domain Archaea. They exhibit extraordinary ecological, biochemical, physiological characteristics colorbox{yellow}{and have a huge biotechnological potential}. Yet, the only possibility to assess the methane (CH$_4$ production potential of hydrogenotrophic methanogens is to apply gas chromatographic quantification of CH$_4$.In order to be able to effectively screen pure cultures of hydrogenotrophic methanogens regarding their CH$_4$ production potential we developed a novel method for indirect quantification of colorbox{yellow}{the} volumetric CH$_4$ production rate by measuring colorbox{yellow}{the} volumetric water production rate. This colorbox{yellow}{ } method was established in serum bottles for cultivation of methanogens in closed batch cultivation mode. Water production was colorbox{yellow}{estimated} by determining the difference in mass increase in an isobaric setting.This novel CH$_4$ quantification method is an accurate and precise analytical technique, colorbox{yellow}{which can be used} to rapidly screen pure cultures of methanogens regarding colorbox{yellow}{their} volumetric CH$_{4}$ evolution rate. colorbox{yellow}{It} is a cost effective alternative colorbox{yellow}{determining} CH$_4$ production of methanogens over CH$_4$ quantification by using gas chromatography, especially if colorbox{yellow}{ } applied as a high throughput quantification method. colorbox{yellow}{Eventually, the} method can be universally applied for quantification of CH$_4$ production from psychrophilic, thermophilic and hyperthermophilic hydrogenotrophic methanogens.

  17. Seafood Spoilage Predictor - development and distribution of a product specific application software

    DEFF Research Database (Denmark)

    Dalgaard, Paw; Buch, P.; Silberg, Steen

    2002-01-01

    To allow shelf-life prediction of a range of products, the Seafood Spoilage Predictor (SSP) software has been developed to include both kinetic models for growth of specific spoilage microorganisms and empirical relative rates of spoilage models. SSP can read and evaluate temperature profile data...

  18. Production of specific structured lipids by enzymatic interesterification: optimization of the reaction by response surface design

    DEFF Research Database (Denmark)

    Xu, Xuebing; Skands, Anja Rebecca Havegaard; Adler-Nissen, Jens

    1998-01-01

    Rapeseed oil and capric acid were interesterified in solvent-free media catalyzed by Lipozyme IM (Rhizomucor miehei) to produce specific-structured lipids (SSLs). The process was optimized by response surface design concerning the effects of acyl migration and the by-products of diacylglycerols...

  19. Application of a path sensitizing method on automated generation of test specifications for control software

    International Nuclear Information System (INIS)

    Morimoto, Yuuichi; Fukuda, Mitsuko

    1995-01-01

    An automated generation method for test specifications has been developed for sequential control software in plant control equipment. Sequential control software can be represented as sequential circuits. The control software implemented in a control equipment is designed from these circuit diagrams. In logic tests of VLSI's, path sensitizing methods are widely used to generate test specifications. But the method generates test specifications at a single time only, and can not be directly applied to sequential control software. The basic idea of the proposed method is as follows. Specifications of each logic operator in the diagrams are defined in the software design process. Therefore, test specifications of each operator in the control software can be determined from these specifications, and validity of software can be judged by inspecting all of the operators in the logic circuit diagrams. Candidates for sensitized paths, on which test data for each operator propagates, can be generated by the path sensitizing method. To confirm feasibility of the method, it was experimentally applied to control software in digital control equipment. The program could generate test specifications exactly, and feasibility of the method was confirmed. (orig.) (3 refs., 7 figs.)

  20. Disinfection By-Product Exposures and the Risk of Specific Cardiac Birth Defects

    Science.gov (United States)

    Wright, J. Michael; Evans, Amanda; Kaufman, John A.; Rivera-Núñez, Zorimar; Narotsky, Michael G.

    2016-01-01

    Background: Epidemiological studies suggest that women exposed to disinfection by-products (DBPs) have an increased risk of delivering babies with cardiovascular defects (CVDs). Objective: We examined nine CVDs in relation to categorical DBP exposures including bromoform, chloroform, dibromochloromethane (DBCM), bromodichloromethane (BDCM), monobromoacetic acid (MBAA), dichloroacetic acid (DCAA), trichloroacetic acid (TCAA), and summary DBP measures (HAA5, THMBr, THM4, and DBP9). Methods: We calculated adjusted odds ratios (aORs) in a case–control study of birth defects in Massachusetts with complete quarterly 1999–2004 trihalomethane (THM) and haloacetic acid (HAA) data. We randomly matched 10 controls each to 904 CVD cases based on week of conception. Weight-averaged aggregate first-trimester DBP exposures were assigned to individuals based on residence at birth. Results: We detected associations for tetralogy of Fallot and the upper exposure categories for TCAA, DCAA, and HAA5 (aOR range, 3.34–6.51) including positive exposure–response relationships for DCAA and HAA5. aORs consistent in magnitude were detected between atrial septal defects and bromoform (aOR = 1.56; 95% CI: 1.01, 2.43), as well as DBCM, chloroform, and THM4 (aOR range, 1.26–1.67). Ventricular septal defects (VSDs) were associated with the highest bromoform (aOR = 1.85; 95% CI: 1.20, 2.83), MBAA (aOR = 1.81; 95% CI: 0.85, 3.84), and DBCM (aOR = 1.54; 95% CI: 1.00, 2.37) exposure categories. Conclusions: To our knowledge, this is the first birth defect study to develop multi-DBP adjusted regression models as well as the first CVD study to evaluate HAA exposures and the second to evaluate bromoform exposures. Our findings, therefore, inform exposure specificity for the consistent associations previously reported between THM4 and CVDs including VSDs. Citation: Wright JM, Evans A, Kaufman JA, Rivera-Núñez Z, Narotsky MG. 2017. Disinfection by-product exposures and the risk of specific

  1. Production of prostate-specific antigen by a breast cancer cell line, Sk-Br-3

    International Nuclear Information System (INIS)

    Kamali Sarvestani, E.; Ghaderi, A.

    2002-01-01

    Prostate-specific antigen is a 33-KDa serine protease that is produced predominantly by prostate epithelium. However, it has been shown that about 30-40% of female breast tumors produce prostate-specific antigen and its production is associated with the presence of estrogen and progesterone receptors. We have now developed a new tissue culture system to study prostate-specific antigen production in breast cancer and its association with prognostic factors such as progesterone receptor and c-erbB-2. For this purpose we investigated the ability of prostate-specific antigen production in five different cell lines, including two breast cancer cell lines, Sk-Br-3 and MDA-MB-453. The prostate-specific antigen in tissue culture supernatant and cytoplasm of the Sk-Br-3 cell line was detected by western blotting and immunoperoxidase, respectively. Furthermore, we found lower expression of c-erbB-2 in Sk-Br-3 than non-prostate-specific antigen producer breast cancer cell line, MDA-MB-453. Progesterone receptor was expressed by both prostate-specific antigen-positive and -negative cell lines and only the intensity of staining and the number of positive cells in Sk-Br-3 population was higher than MDA-MB-453. According to our findings prostate-specific antigen can be considered as a good prognostic factor in breast cancer and we suggest that these two cell lines are a good in vitro model to study the relationship of different breast cancer prognostic factors and their regulations

  2. State-Specific Prevalence of Tobacco Product Use Among Adults - United States, 2014-2015.

    Science.gov (United States)

    Odani, Satomi; Armour, Brian S; Graffunder, Corinne M; Willis, Gordon; Hartman, Anne M; Agaku, Israel T

    2018-01-26

    Despite recent declines in cigarette smoking prevalence, the tobacco product landscape has shifted to include emerging tobacco products* (1,2). Previous research has documented adult use of smokeless tobacco and cigarettes by state (3); however, state-specific data on other tobacco products are limited. To assess tobacco product use in the 50 U.S. states and the District of Columbia (DC), CDC and the National Cancer Institute analyzed self-reported use of six tobacco product types: cigarettes, cigars, regular pipes, water pipes, electronic cigarettes (e-cigarettes), and smokeless tobacco products among adults aged ≥18 years using data from the 2014-2015 Tobacco Use Supplement to the Current Population Survey (TUS-CPS). Prevalence of ever-use of any tobacco product ranged from 27.0% (Utah) to 55.4% (Wyoming). Current (every day or some days) use of any tobacco product ranged from 10.2% (California) to 27.7% (Wyoming). Cigarettes were the most common currently used tobacco product in all states and DC. Among current cigarette smokers, the proportion who currently used one or more other tobacco products ranged from 11.5% (Delaware) to 32.3% (Oregon). Differences in tobacco product use across states underscore the importance of implementing proven population-level strategies to reduce tobacco use and expanding these strategies to cover all forms of tobacco marketed in the United States. Such strategies could include comprehensive smoke-free policies, tobacco product price increases, anti-tobacco mass media campaigns, and barrier-free access to clinical smoking cessation resources (1,4).

  3. Adjective production by Russian-speaking children with specific language impairment.

    Science.gov (United States)

    Tribushinina, Elena; Dubinkina, Elena

    2012-06-01

    Research on specific language impairment (SLI) has primarily focused on the acquisition of nouns and verbs. Less attention has been given to other content-word classes, such as adjectives and adverbs. This article investigates adjective production by 7- to 10-year-old Russian-speaking children with SLI and their typically developing (TD) peers and focuses on the production of antonymous adjectives and degree markers in an elicitation experiment. The results show that degree morphology is more impaired in SLI than antonymy. In antonym production, children with SLI were able to catch up with their TD peers by age 8. In the domain of degree, however, the SLI group lagged behind the TD controls across all ages studied. Error analysis indicates that language-impaired children have particular difficulty with agreement inflection and affixal negations. They also substitute adjectives with specific meanings by more general terms. The implications of this study for the morphological-richness hypothesis are discussed.

  4. Review of nonchemical methods for controlling stored products pests

    International Nuclear Information System (INIS)

    Ignatowicz, S.

    1996-01-01

    Fumigation of stored products with methyl bromide has been an important means of limiting the loss of quality and quantity of these commodities that are subject to attack by cosmopolitan stored product pests. Methyl bromide was identified as a substance depleting ozone, and is expected to be withdrawn from production, importation, and use in Poland and other countries soon after 2000. Based on the current knowledge, most of alternatives to methyl bromide (controlled atmospheres, heat, cold, irradiation, biotechnical methods, inert dusts, biological methods, sanitation) have researchable gaps or other constraints. None of these alternatives used alone will replace methyl bromide. Successful pest control in the absence of methyl bromide will require the development of sophisticated pest monitoring and decision support systems to enable the use of integrated pest management strategies. (author)

  5. Research on methods of radioisotope production in CEFR

    International Nuclear Information System (INIS)

    Yu Hong

    2003-01-01

    The methods of isotope production in CEFR (China Experimental Fast Reactor) and the preliminary design of isotope assembly were introduced briefly in the paper. Isotope 32 P, 33 P, 35 S and 89 Sr can be produced in core area and 14 C, 51 Cr and 60 Co can be produced in radial shield area in CEFR with special isotope assembly. (author)

  6. Host cells and methods for production of isobutanol

    Science.gov (United States)

    Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa; Okeefe, Daniel P.; Kruckeberg, Arthur Leo; Li, Yougen; Maggio-Hall, Lori; McElvain, Jessica; Nelson, Mark J.; Patnaik, Ranjan; Rothman, Steven Cary

    2017-10-17

    Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

  7. The contemporary art of cost management methods during product development

    NARCIS (Netherlands)

    Wouters, M.; Morales, S.; Epstein, M.J.; Lee, J.Y.

    2014-01-01

    Purpose To provide an overview of research published in the management accounting literature on methods for cost management in new product development, such as a target costing, life cycle costing, component commonality, and modular design. Methodology/approach The structured literature search

  8. Systemic representation of a production system: the Sagace method

    International Nuclear Information System (INIS)

    Benkhannouche, S.; Penalva, J.M.

    1993-01-01

    The systemic approach used in this study is devoted at a high level radioactive waste vitrification pilot. The Sagace method permits to realize a global view of this pilot plant taking in account the process, the production subsystems and the control process subsystems. 4 figs., 4 refs

  9. Efficiency analysis of hydrogen production methods from biomass

    NARCIS (Netherlands)

    Ptasinski, K.J.

    2008-01-01

    Abstract: Hydrogen is considered as a universal energy carrier for the future, and biomass has the potential to become a sustainable source of hydrogen. This article presents an efficiency analysis of hydrogen production processes from a variety of biomass feedstocks by a thermochemical method

  10. A Comparison of Assessment Methods and Raters in Product Creativity

    Science.gov (United States)

    Lu, Chia-Chen; Luh, Ding-Bang

    2012-01-01

    Although previous studies have attempted to use different experiences of raters to rate product creativity by adopting the Consensus Assessment Method (CAT) approach, the validity of replacing CAT with another measurement tool has not been adequately tested. This study aimed to compare raters with different levels of experience (expert ves.…

  11. Production method of {alpha} particles; Une methode de production des particules {alpha}

    Energy Technology Data Exchange (ETDEWEB)

    Prevot, F [Commissariat a l' Energie Atomique, Saclay(France). Centre d' Etudes Nucleaires

    1953-07-01

    It is proposed a method to get an intense beam of {alpha} particles. With a source of ordinary ions, we form a helium beam, once ionized, it is accelerated with an energy of a few hundreds of keV. While crossing a matter any that can be a thin leaf or a gaseous blade, the second electron of helium is pulled with a yield that only depends on the energy of the beam of helium and that is equal to 1/2 for 650 keV. (author) [French] Il est propose une methode pour obtenir un faisceau intense de particules {alpha}. Avec une source d'ions ordinaire, on forme un faisceau d'helium une fois ionise qu'on accelere avec une energie de quelques centaines de keV. En traversant une matiere quelconque qui peut etre sous forme de feuille mince ou de lame gazeuse, le deuxieme electron de l'helium est arrache avec un rendement qui ne depend que de l'energie du faisceau d'helium et qui vaut 1/2 pour 650 keV. (auteur)

  12. A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment.

    Science.gov (United States)

    Fu, Wei; Zhu, Pengyu; Wang, Chenguang; Huang, Kunlun; Du, Zhixin; Tian, Wenying; Wang, Qin; Wang, Huiyu; Xu, Wentao; Zhu, Shuifang

    2015-08-04

    Digital PCR has developed rapidly since it was first reported in the 1990 s. It was recently reported that an improved method facilitated the detection of genetically modified organisms (GMOs). However, to use this improved method, the samples must be pretreated, which could introduce inaccuracy into the results. In our study, we explored a pretreatment-free digital PCR detection method for the screening for GMOs. We chose the CaMV35s promoter and the NOS terminator as the templates in our assay. To determine the specificity of our method, 9 events of GMOs were collected, including MON810, MON863, TC1507, MIR604, MIR162, GA21, T25, NK603 and Bt176. Moreover, the sensitivity, intra-laboratory and inter-laboratory reproducibility of our detection method were assessed. The results showed that the limit of detection of our method was 0.1%, which was lower than the labeling threshold level of the EU. The specificity and stability among the 9 events were consistent, respectively. The intra-laboratory and inter-laboratory reproducibility were both good. Finally, the perfect fitness for the detection of eight double-blind samples indicated the good practicability of our method. In conclusion, the method in our study would allow more sensitive, specific and stable screening detection of the GMO content of international trading products.

  13. Electrochemical method applicable to treatment of wastewater from nitrotriazolone production.

    Science.gov (United States)

    Wallace, Lynne; Cronin, Michael P; Day, Anthony I; Buck, Damian P

    2009-03-15

    Laboratory studies show that electrochemical oxidation of acidic nitrotriazolone (NTO) solutions results in complete mineralization, with ammonium nitrate as the only solution product Other products (carbon dioxide, carbon monoxide, and nitrous oxide) are eliminated as gases from the working electrode. No additional chemical loading is required for the process, and electricity isthe only input The process maytherefore represent a cost-effective and environmentally friendly method of remediation for wastewater from NTO manufacture. Electrolyses were carried out at different applied voltages and at NTO concentrations of 0.01 and 0.05 mol/L, and the results indicate that a higher oxidation rate results in a greater charge passed per mole of NTO oxidized and increased production of nitrous oxide. Mechanisms are proposed on the basis of competing oxidative pathways that account for all products formed and the total charge passed during the reaction.

  14. Method of processing liquid waste containing fission product

    International Nuclear Information System (INIS)

    Funabashi, Kiyomi; Kawamura, Fumio; Matsuda, Masami; Komori, Itaru; Miura, Eiichi.

    1988-01-01

    Purpose: To prepare solidification products of low surface dose by removing cesium which is main radioactive nuclides from re-processing plants. Method: Liquid wastes containing a great amount of fission products are generated accompanying the reprocessing for spent nuclear fuels. After pH adjustment, the liquid wastes are sent to a concentrator to concentrate the dissolved ingredients. The concentrated liquid wastes are pumped to an adsorption tower in which radioactive cesium contributing much to the surface dose is removed. Then, the liquid wastes are sent by way of a surge tank to a mixing tank, in which they are mixed under stirring with solidifying agents such as cements. Then, the mixture is filled in a drum-can and solidified. According to this invention, since radioactive cesium is removed before solidification, it is possible to prepare solidification products at low surface dose and facilitate the handling of the solidification products. (Horiuchi, T.)

  15. Interannual Variation in Phytoplankton Class-specific Primary Production at a Global Scale

    Science.gov (United States)

    Rousseaux, Cecile; Gregg, Watson

    2014-01-01

    Phytoplankton is responsible for over half of the net primary production on earth. The knowledge on the contribution of various phytoplankton groups to the total primary production is still poorly understood. Data from satellite observations suggest that for upwelling regions, photosynthetic rates by microplankton is higher than that of nanoplankton but that when the spatial extent is considered, the production by nanoplankton is comparable or even larger than microplankton. Here, we used the NASA Ocean Biogeochemical Model (NOBM) combined with remote sensing data via assimilation to evaluate the contribution of 4 phytoplankton groups to the total primary production. Globally, diatoms were the group that contributed the most to the total phytoplankton production (approx. 50%) followed by coccolithophores and chlorophytes. Primary production by diatoms was highest in high latitude (>45 deg) and in major upwelling systems (Equatorial Pacific and Benguela system). We assessed the effects of climate variability on the class-specific primary production using global (i.e. Multivariate El Nino Index, MEI) and 'regional' climate indices (e.g. Southern Annular Mode (SAM), Pacific Decadal Oscillation (PDO) and North Atlantic Oscillation (NAO)). Most interannual variability occurred in the Equatorial Pacific and was associated with climate variability. These results provide a modeling and data assimilation perspective to phytoplankton partitioning of primary production and contribute to our understanding of the dynamics of the carbon cycle in the oceans at a global scale.

  16. Method s for Measuring Productivity in Libraries and Information Centres

    Directory of Open Access Journals (Sweden)

    Mohammad Alaaei

    2009-04-01

    Full Text Available   Within Information centers, productivity is the result of optimal and effective use of information resources, service quality improvement, increased user satisfaction, pleasantness of working environment, increased motivation and enthusiasm of staff to work better. All contribute to the growth and development of information centers. Thus these centers would need to be familiar with methods employed in productivity measurement. Productivity is one of the criteria for evaluating system performance. In the past decades particular emphasis has been placed on measurement and improvement of human resource, creativity, innovation and expert analysis. Contemplation and efforts made towards identification of problems and issues and new means to make more useful and better resource management is the very definition of productivity. Simply put, productivity is the relationship between system output and the elements garnered to produce these outputs. The causality between variables and factors impacting on productivity is very complex. In information centers, given the large volume of elements involved, it seems necessary to increase efficiency and productivity

  17. Methods for forming complex oxidation reaction products including superconducting articles

    International Nuclear Information System (INIS)

    Rapp, R.A.; Urquhart, A.W.; Nagelberg, A.S.; Newkirk, M.S.

    1992-01-01

    This patent describes a method for producing a superconducting complex oxidation reaction product of two or more metals in an oxidized state. It comprises positioning at least one parent metal source comprising one of the metals adjacent to a permeable mass comprising at least one metal-containing compound capable of reaction to form the complex oxidation reaction product in step below, the metal component of the at least one metal-containing compound comprising at least a second of the two or more metals, and orienting the parent metal source and the permeable mass relative to each other so that formation of the complex oxidation reaction product will occur in a direction towards and into the permeable mass; and heating the parent metal source in the presence of an oxidant to a temperature region above its melting point to form a body of molten parent metal to permit infiltration and reaction of the molten parent metal into the permeable mass and with the oxidant and the at least one metal-containing compound to form the complex oxidation reaction product, and progressively drawing the molten parent metal source through the complex oxidation reaction product towards the oxidant and towards and into the adjacent permeable mass so that fresh complex oxidation reaction product continues to form within the permeable mass; and recovering the resulting complex oxidation reaction product

  18. Comparative analysis of chemical similarity methods for modular natural products with a hypothetical structure enumeration algorithm.

    Science.gov (United States)

    Skinnider, Michael A; Dejong, Chris A; Franczak, Brian C; McNicholas, Paul D; Magarvey, Nathan A

    2017-08-16

    Natural products represent a prominent source of pharmaceutically and industrially important agents. Calculating the chemical similarity of two molecules is a central task in cheminformatics, with applications at multiple stages of the drug discovery pipeline. Quantifying the similarity of natural products is a particularly important problem, as the biological activities of these molecules have been extensively optimized by natural selection. The large and structurally complex scaffolds of natural products distinguish their physical and chemical properties from those of synthetic compounds. However, no analysis of the performance of existing methods for molecular similarity calculation specific to natural products has been reported to date. Here, we present LEMONS, an algorithm for the enumeration of hypothetical modular natural product structures. We leverage this algorithm to conduct a comparative analysis of molecular similarity methods within the unique chemical space occupied by modular natural products using controlled synthetic data, and comprehensively investigate the impact of diverse biosynthetic parameters on similarity search. We additionally investigate a recently described algorithm for natural product retrobiosynthesis and alignment, and find that when rule-based retrobiosynthesis can be applied, this approach outperforms conventional two-dimensional fingerprints, suggesting it may represent a valuable approach for the targeted exploration of natural product chemical space and microbial genome mining. Our open-source algorithm is an extensible method of enumerating hypothetical natural product structures with diverse potential applications in bioinformatics.

  19. Comprehensive evaluation method in application of nuclear DCS product design

    International Nuclear Information System (INIS)

    Wang Weixin; Zhao Zhemin; Shi Yingbin

    2014-01-01

    In order to select the best design proposal in short time, the TOPSIS comprehensive evaluation method in the nuclear power plant DCS product design was introduced. It can intuitively show the different design proposals good or not good by data and shorten the time of the design proposal optimization. The design proposal selected by this method will be more reasonable and has good comprehensive performance indexes. The TOPSIS comprehensive evaluation method achieves good result in one of the nuclear power plant DCS cabinet design proposal optimization. (authors)

  20. Production of polymer nanomembranes by super deep penetration method

    OpenAIRE

    Figovsky, Oleg; Gotlib, Elena; Pashin, Dmitry; Makeev, Alexander

    2012-01-01

    A new super deep penetration (SDP) method of polymer nanomembranes production was developed. Its advantages as compared to nuclear methods were discussed. They are connected with simplicity and low price of SDP application. SDP method implies the use of explosion for track membranes forming. As a result, a plurality of holes are formed in polymer matrixes. Removal of the residual particles of water-soluble salt is done by washing the pierced membrane with water. Розроблено новий метод надг...

  1. DEVELOPMENT OF TOFU PRODUCTION METHOD WITH PROBIOTIC BACTERIA ADDITION

    Directory of Open Access Journals (Sweden)

    Dorota Zielińska

    2015-06-01

    Full Text Available The aim of the study was to develop a production method for tofu with probiotic bacteria under laboratory conditions. The works included: selection of a strain and tofu production conditions, and a storage test of the manufactured product. It was concluded that the sensory quality of tofu with the addition of different probiotic cultures did not differ significantly (p>0.01, depending on used strains and their mixtures, and the sample quality was comparable to the commercial product. It was observed that the number of Lactobacillus bacteria in study samples was the factor determining the palatability of tofu (r= 0.75. On the other hand, the sensory quality of products was significantly affected by the production method of tofu with the addition of probiotic bacteria. It was concluded that the formation of curds from soy beverage by the addition of CaSO4, followed by inoculation with Lactobacillus casei ŁOCK 0900 at the amount of 9.26 log CFU/g and incubation at temp. of 37C for 2h as well as for 20h are methods recommended for production tofu with regard to sensory qualities of the final product among all tested methods. The number of lactic acid bacteria in studied tofu samples was maintained at the high level (109-1010 CFU/g, and the number of Bifidobacterium animalis ssp lactis BB-12 bacteria did not exceed 103 CFU/g, whereas the number of Lactobacillus bacteria was equal to 108-109 CFU/g. For the period of 15 days of storage of tofu with probiotic bacteria at the temperature of 4C the number of lactic acid bacteria was maintained at the constant level of approx. 109 CFU/g. It was concluded that it is possible to produce tofu with probiotic bacteria that has acceptable sensory characteristics and a high number of lactic acid bacteria, therefore the product could be considered as a functional one.

  2. A modular modulation method for achieving increases in metabolite production.

    Science.gov (United States)

    Acerenza, Luis; Monzon, Pablo; Ortega, Fernando

    2015-01-01

    Increasing the production of overproducing strains represents a great challenge. Here, we develop a modular modulation method to determine the key steps for genetic manipulation to increase metabolite production. The method consists of three steps: (i) modularization of the metabolic network into two modules connected by linking metabolites, (ii) change in the activity of the modules using auxiliary rates producing or consuming the linking metabolites in appropriate proportions and (iii) determination of the key modules and steps to increase production. The mathematical formulation of the method in matrix form shows that it may be applied to metabolic networks of any structure and size, with reactions showing any kind of rate laws. The results are valid for any type of conservation relationships in the metabolite concentrations or interactions between modules. The activity of the module may, in principle, be changed by any large factor. The method may be applied recursively or combined with other methods devised to perform fine searches in smaller regions. In practice, it is implemented by integrating to the producer strain heterologous reactions or synthetic pathways producing or consuming the linking metabolites. The new procedure may contribute to develop metabolic engineering into a more systematic practice. © 2015 American Institute of Chemical Engineers.

  3. Microbiological specifications and testing methods for irradiated food. Report of a panel of experts

    International Nuclear Information System (INIS)

    1970-01-01

    In recent years there has been increased interest in the development of food items processed by means of ionizing radiation, wherever that form of preservation might show advantage over other methods. If this method becomes successful, the various items will be commercially exploited; it would then be convenient to have similar legislation in many countries of the world to control this type of processing and to facilitate international trade. To gather information and suggestions in order to devise legislation on irradiated food, a Joint FAO/IAEA/ WHO Expert Committee on the Technical Basis for Legislation on Irradiated Food was held in Rome on 21-28 April 1964. The Committee's report, 'The technical basis for legislation on irradiated food', was published as FAO Atomic Energy Series No.6 and WHO Technical Series No. 316. The Committee's terms of reference were to consider the available evidence on the effect on food of treatment with ionizing radiation in the context of wholesomeness and safety for consumption. Nutritional aspects were taken into account, but microbiological safety and microbiological methods for irradiated food were excluded since these subjects were believed to be too broad for adequate coverage in the working time available at the meeting. However, realizing the importance of radiation microbiology and the need for guidance in formulating regulations in this field, the committee drafted the following recommendation: 'The methods and standards used to ensure the microbiological safety of the irradiated product should, as a matter of urgency, be subject to review by competent international bodies in order that internationally acceptable methods and standards may be agreed upon'. In response to this recommendation an FAO/IAEA Panel on Microbiological Standards and Testing Methods for Irradiated Food was held in Vienna on 22-26 June 1965, in collaboration with the International Association of Microbiological Societies (IAMS). This was a working

  4. Microbiological specifications and testing methods for irradiated food. Report of a panel of experts

    International Nuclear Information System (INIS)

    1971-01-01

    In recent years there has been increased interest in the development of food items processed by means of ionizing radiation, wherever that form of preservation might show advantage over other methods. If this method becomes successful, the various items will be commercially exploited; it would then be convenient to have similar legislation in many countries of the world to control this type of processing and to facilitate international trade. To gather information and suggestions in order to devise legislation on irradiated food, a Joint FAO/IAEA/ WHO Expert Committee on the Technical Basis for Legislation on Irradiated Food was held in Rome on 21-28 April 1964. The Committee's report, 'The technical basis for legislation on irradiated food', was published as FAO Atomic Energy Series No.6 and WHO Technical Series No. 316. The Committee's terms of reference were to consider the available evidence on the effect on food of treatment with ionizing radiation in the context of wholesomeness and safety for consumption. Nutritional aspects were taken into account, but microbiological safety and microbiological methods for irradiated food were excluded since these subjects were believed to be too broad for adequate coverage in the working time available at the meeting. However, realizing the importance of radiation microbiology and the need for guidance in formulating regulations in this field, the committee drafted the following recommendation: 'The methods and standards used to ensure the microbiological safety of the irradiated product should, as a matter of urgency, be subject to review by competent international bodies in order that internationally acceptable methods and standards may be agreed upon'. In response to this recommendation an FAO/IAEA Panel on Microbiological Standards and Testing Methods for Irradiated Food was held in Vienna on 22-26 June 1965, in collaboration with the International Association of Microbiological Societies (IAMS). This was a working

  5. Microbiological specifications and testing methods for irradiated food. Report of a panel of experts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1970-04-01

    In recent years there has been increased interest in the development of food items processed by means of ionizing radiation, wherever that form of preservation might show advantage over other methods. If this method becomes successful, the various items will be commercially exploited; it would then be convenient to have similar legislation in many countries of the world to control this type of processing and to facilitate international trade. To gather information and suggestions in order to devise legislation on irradiated food, a Joint FAO/IAEA/ WHO Expert Committee on the Technical Basis for Legislation on Irradiated Food was held in Rome on 21-28 April 1964. The Committee's report, 'The technical basis for legislation on irradiated food', was published as FAO Atomic Energy Series No.6 and WHO Technical Series No. 316. The Committee's terms of reference were to consider the available evidence on the effect on food of treatment with ionizing radiation in the context of wholesomeness and safety for consumption. Nutritional aspects were taken into account, but microbiological safety and microbiological methods for irradiated food were excluded since these subjects were believed to be too broad for adequate coverage in the working time available at the meeting. However, realizing the importance of radiation microbiology and the need for guidance in formulating regulations in this field, the committee drafted the following recommendation: 'The methods and standards used to ensure the microbiological safety of the irradiated product should, as a matter of urgency, be subject to review by competent international bodies in order that internationally acceptable methods and standards may be agreed upon'. In response to this recommendation an FAO/IAEA Panel on Microbiological Standards and Testing Methods for Irradiated Food was held in Vienna on 22-26 June 1965, in collaboration with the International Association of Microbiological Societies (IAMS). This was a working

  6. Extension classification method for low-carbon product cases

    Directory of Open Access Journals (Sweden)

    Yanwei Zhao

    2016-05-01

    Full Text Available In product low-carbon design, intelligent decision systems integrated with certain classification algorithms recommend the existing design cases to designers. However, these systems mostly dependent on prior experience, and product designers not only expect to get a satisfactory case from an intelligent system but also hope to achieve assistance in modifying unsatisfactory cases. In this article, we proposed a new categorization method composed of static and dynamic classification based on extension theory. This classification method can be integrated into case-based reasoning system to get accurate classification results and to inform designers of detailed information about unsatisfactory cases. First, we establish the static classification model for cases by dependent function in a hierarchical structure. Then for dynamic classification, we make transformation for cases based on case model, attributes, attribute values, and dependent function, thus cases can take qualitative changes. Finally, the applicability of proposed method is demonstrated through a case study of screw air compressor cases.

  7. Operational Excellence through Schedule Optimization and Production Simulation of Application Specific Integrated Circuits.

    Energy Technology Data Exchange (ETDEWEB)

    Flory, John Andrew [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Padilla, Denise D. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Gauthier, John H. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Zwerneman, April Marie [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Miller, Steven P [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2016-05-01

    Upcoming weapon programs require an aggressive increase in Application Specific Integrated Circuit (ASIC) production at Sandia National Laboratories (SNL). SNL has developed unique modeling and optimization tools that have been instrumental in improving ASIC production productivity and efficiency, identifying optimal operational and tactical execution plans under resource constraints, and providing confidence in successful mission execution. With ten products and unprecedented levels of demand, a single set of shared resources, highly variable processes, and the need for external supplier task synchronization, scheduling is an integral part of successful manufacturing. The scheduler uses an iterative multi-objective genetic algorithm and a multi-dimensional performance evaluator. Schedule feasibility is assessed using a discrete event simulation (DES) that incorporates operational uncertainty, variability, and resource availability. The tools provide rapid scenario assessments and responses to variances in the operational environment, and have been used to inform major equipment investments and workforce planning decisions in multiple SNL facilities.

  8. Contribution to the study of radioisotopic methods in pharmacokinetics. Application to specific determinations of drugs or their metabolites

    International Nuclear Information System (INIS)

    Khiat, Mouloud.

    1977-01-01

    The aim of this work was to refute one of the major criticisms expressed on the used of labelled molecules, that they give an overall result. Techniques were therefore developed to determine quantitatively and specifically the kinetics of the drug itself or its metabolites. Two methods turning to account the great sensitivity and facility offered by labelled molecules have been adopted: - reverse isotopic dilution and double isotopic dilution, applied to some medicinal molecules. In part one the glipentide labelled molecule was used to measure the unchanged product in rat plasma: the kinetics are established. In part two the plasma fraction curves of unchanged products and their metabolites were studied for two molecules of similar structure: cyclobutane carboxylic acid and propyl-3 cyclobutane carboxylic acid. Finally a radiocompetitive method to determine a sulfamido-benzoic diuretic, based on the interaction with carbonic anhydrase, was investigated. The sensitivity of these radioisotopic methods depends on the specific activity of the labelled molecule. For the glipentide for instance, where the specific activity is very high, as little as 2 ng/ml of plasma can be determined. The specific activities of cyclobutane carboxylic, propyl-3 cyclobutane carboxylic and sulfamido-3 chloro-4 benzoic acids are not high enough for measurements better than 1 μg/ml plasma to be obtained [fr

  9. Accuracy increase of the coordinate measurement based on the model production of geometrical parts specifications

    Science.gov (United States)

    Zlatkina, O. Yu

    2018-04-01

    There is a relationship between the service properties of component parts and their geometry; therefore, to predict and control the operational characteristics of parts and machines, it is necessary to measure their geometrical specifications. In modern production, a coordinate measuring machine is the advanced measuring instrument of the products geometrical specifications. The analysis of publications has shown that during the coordinate measurements the problems of choosing locating chart of parts and coordination have not been sufficiently studied. A special role in the coordination of the part is played by the coordinate axes informational content. Informational content is the sum of the degrees of freedom limited by the elementary item of a part. The coordinate planes of a rectangular coordinate system have different informational content (three, two, and one). The coordinate axes have informational content of four, two and zero. The higher the informational content of the coordinate plane or axis, the higher its priority for reading angular and linear coordinates is. The geometrical model production of the coordinate measurements object taking into account the information content of coordinate planes and coordinate axes allows us to clearly reveal the interrelationship of the coordinates of the deviations in location, sizes and deviations of their surfaces shape. The geometrical model helps to select the optimal locating chart of parts for bringing the machine coordinate system to the part coordinate system. The article presents an algorithm the model production of geometrical specifications using the example of the piston rod of a compressor.

  10. The production of direct object clitics in pre-school- and primary school-aged children with specific language impairments.

    Science.gov (United States)

    Guasti, Maria Teresa; Palma, Silvia; Genovese, Elisabetta; Stagi, Paolo; Saladini, Gabriella; Arosio, Fabrizio

    2016-01-01

    Third-person direct object (DO) clitic pronoun production is examined through an elicited production method in pre-school- and primary school-aged groups of Italian children with specific language impairment (SLI) to establish whether there is an improvement from age 5 years to age 7 years and whether there are qualitative differences in the two groups' responses. It was found that 5- and 7-year-old Italian children with SLI produce fewer third-person DO clitics than same-age peers. The kind of responses they provide changes: at 5 years, children with SLI tend to omit clitics, while at 7 years, they use a full noun. Production of third-person DO clitics is a persistent challenge for children with SLI and is confirmed to be a good clinical marker both at 5 and 7 years of age.

  11. Comparison of methods applicable to evaluation of nuclear power plant technical specifications

    International Nuclear Information System (INIS)

    Cho, N.Z.; Bozoki, G.E.; Youngblood, R.W.

    1986-01-01

    This study compares three probabilistic methods based on the static fault tree analysis, time-dependent unavailability analysis, and Markov analysis, which can be used to evaluate technical specifications in nuclear power plants. They are tested on a sample problem which was devised to closely represent the important and essential characteristics that should be addressed in determination and evaluation of the technical specifications

  12. Engineered microbes and methods for microbial oil production

    Energy Technology Data Exchange (ETDEWEB)

    Stephanopoulos, Gregory; Tai, Mitchell; Chakraborty, Sagar

    2018-01-09

    Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.

  13. Systems and methods for separation and purification of products

    Energy Technology Data Exchange (ETDEWEB)

    Weiss, Michael Joseph; Gilliam, Ryan J.; Self, Kyle; Mariansky, Gal; Leclerc, Margarete K.; Shipchandler, Riyaz Mohammed; Nagar, Jacob

    2017-11-28

    There are provided methods and systems for an electrochemical cell including an anode and a cathode where the anode is contacted with a metal ion that converts the metal ion from a lower oxidation state to a higher oxidation state. The metal ion in the higher oxidation state is reacted with an unsaturated hydrocarbon and/or a saturated hydrocarbon to form products. Separation and/or purification of the products as well as of the metal ions in the lower oxidation state and the higher oxidation state, is provided herein.

  14. New Approaches to Aluminum Integral Foam Production with Casting Methods

    Directory of Open Access Journals (Sweden)

    Ahmet Güner

    2015-08-01

    Full Text Available Integral foam has been used in the production of polymer materials for a long time. Metal integral foam casting systems are obtained by transferring and adapting polymer injection technology. Metal integral foam produced by casting has a solid skin at the surface and a foam core. Producing near-net shape reduces production expenses. Insurance companies nowadays want the automotive industry to use metallic foam parts because of their higher impact energy absorption properties. In this paper, manufacturing processes of aluminum integral foam with casting methods will be discussed.

  15. Engineered microbes and methods for microbial oil production

    Science.gov (United States)

    Stephanopoulos, Gregory; Tai, Mitchell; Chakraborty, Sagar

    2015-02-10

    Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.

  16. Method for the production of uranium chloride salt

    Science.gov (United States)

    Westphal, Brian R.; Mariani, Robert D.

    2013-07-02

    A method for the production of UCl.sub.3 salt without the use of hazardous chemicals or multiple apparatuses for synthesis and purification is provided. Uranium metal is combined in a reaction vessel with a metal chloride and a eutectic salt- and heated to a first temperature under vacuum conditions to promote reaction of the uranium metal with the metal chloride for the production of a UCl.sub.3 salt. After the reaction has run substantially to completion, the furnace is heated to a second temperature under vacuum conditions. The second temperature is sufficiently high to selectively vaporize the chloride salts and distill them into a condenser region.

  17. Use of biotechnological methods in the potato seed production

    Directory of Open Access Journals (Sweden)

    Janet Igarza Castro

    2012-01-01

    Full Text Available Potato crop has a large economic importance. Worldwide, propagation of potato by in vitro culture of axillary buds is commonly used in the production of in vitro plants and microtubers. These constitute the core plant material of a production program of potatoes seeds. This study aimed to present a review of scientific literature on the potato propagation by biotechnological methods. This also describes the main characteristics of this crop and the tuberization processes under natural and in vitro conditions. Key words: in vitro plants, microtubers, minitubers, Temporary Inmmersion System.

  18. Waste acceptance product specifications for vitrified high-level waste forms

    International Nuclear Information System (INIS)

    Applewhite-Ramsey, A.; Sproull, J.F.

    1994-01-01

    The Department of Energy (DOE) Office of Environmental Restoration and Waste Management (EM) has developed Waste Acceptance Product Specifications (EM-WAPS). The EM-WAPS will be the basis for defining product acceptance criteria compatible with the requirements of the Civilian Radioactive Waste Management System (CRWMS). The relationship between the EM-WAPS and the CRWMS Systems Requirements document (WA-SRD) will be discussed. The impact of the EM-WAPS on the Savannah River Sit (SRS) Defense Waste Processing Facility's (DWPF) Waste Acceptance Program, Waste Qualification Run planning, and startup schedule will also be reported. 14 refs., 2 tabs

  19. Rapid analysis method for the determination of 14C specific activity in irradiated graphite.

    Directory of Open Access Journals (Sweden)

    Vidmantas Remeikis

    Full Text Available 14C is one of the limiting radionuclides used in the categorization of radioactive graphite waste; this categorization is crucial in selecting the appropriate graphite treatment/disposal method. We propose a rapid analysis method for 14C specific activity determination in small graphite samples in the 1-100 μg range. The method applies an oxidation procedure to the sample, which extracts 14C from the different carbonaceous matrices in a controlled manner. Because this method enables fast online measurement and 14C specific activity evaluation, it can be especially useful for characterizing 14C in irradiated graphite when dismantling graphite moderator and reflector parts, or when sorting radioactive graphite waste from decommissioned nuclear power plants. The proposed rapid method is based on graphite combustion and the subsequent measurement of both CO2 and 14C, using a commercial elemental analyser and the semiconductor detector, respectively. The method was verified using the liquid scintillation counting (LSC technique. The uncertainty of this rapid method is within the acceptable range for radioactive waste characterization purposes. The 14C specific activity determination procedure proposed in this study takes approximately ten minutes, comparing favorably to the more complicated and time consuming LSC method. This method can be potentially used to radiologically characterize radioactive waste or used in biomedical applications when dealing with the specific activity determination of 14C in the sample.

  20. Rapid analysis method for the determination of 14C specific activity in irradiated graphite.

    Science.gov (United States)

    Remeikis, Vidmantas; Lagzdina, Elena; Garbaras, Andrius; Gudelis, Arūnas; Garankin, Jevgenij; Plukienė, Rita; Juodis, Laurynas; Duškesas, Grigorijus; Lingis, Danielius; Abdulajev, Vladimir; Plukis, Artūras

    2018-01-01

    14C is one of the limiting radionuclides used in the categorization of radioactive graphite waste; this categorization is crucial in selecting the appropriate graphite treatment/disposal method. We propose a rapid analysis method for 14C specific activity determination in small graphite samples in the 1-100 μg range. The method applies an oxidation procedure to the sample, which extracts 14C from the different carbonaceous matrices in a controlled manner. Because this method enables fast online measurement and 14C specific activity evaluation, it can be especially useful for characterizing 14C in irradiated graphite when dismantling graphite moderator and reflector parts, or when sorting radioactive graphite waste from decommissioned nuclear power plants. The proposed rapid method is based on graphite combustion and the subsequent measurement of both CO2 and 14C, using a commercial elemental analyser and the semiconductor detector, respectively. The method was verified using the liquid scintillation counting (LSC) technique. The uncertainty of this rapid method is within the acceptable range for radioactive waste characterization purposes. The 14C specific activity determination procedure proposed in this study takes approximately ten minutes, comparing favorably to the more complicated and time consuming LSC method. This method can be potentially used to radiologically characterize radioactive waste or used in biomedical applications when dealing with the specific activity determination of 14C in the sample.

  1. Linearized method: A new approach for kinetic analysis of central dopamine D2 receptor specific binding

    International Nuclear Information System (INIS)

    Watabe, Hiroshi; Hatazawa, Jun; Ishiwata, Kiichi; Ido, Tatsuo; Itoh, Masatoshi; Iwata, Ren; Nakamura, Takashi; Takahashi, Toshihiro; Hatano, Kentaro

    1995-01-01

    The authors proposed a new method (Linearized method) to analyze neuroleptic ligand-receptor specific binding in a human brain using positron emission tomography (PET). They derived the linear equation to solve four rate constants, k 3 , k 4 , k 5 , k 6 from PET data. This method does not demand radioactivity curve in plasma as an input function to brain, and can do fast calculations in order to determine rate constants. They also tested Nonlinearized method including nonlinear equations which is conventional analysis using plasma radioactivity corrected for ligand metabolites as an input function. The authors applied these methods to evaluate dopamine D 2 receptor specific binding of [ 11 C] YM-09151-2. The value of B max /K d = k 3 k 4 obtained by Linearized method was 5.72 ± 3.1 which was consistent with the value of 5.78 ± 3.4 obtained by Nonlinearized method

  2. Dissolution testing of intermediary products in uranium dioxide production by the sol-gel method

    International Nuclear Information System (INIS)

    Melichar, F.; Landspersky, H.; Urbanek, V.

    1979-01-01

    A method was developed of dissolving polyuranates and uranium dioxides in sulphuric acid and in carbonate solutions for testing intermediate products in the sol-gel process preparation of uranium dioxide. A detailed granulometric analysis of spherical particle dispersion was included as part of the tests. Two different production methods were used for the two types of studied materials. The test results show that the test method is suitable for determining temperature sensitivity of the materials to dissolution reaction. The geometrical distribution of impurities in the spherical particles can be determined from the dissolution kinetics. The method allows the determination of the effect of carbon from impurities on the process of uranium dioxide leaching and is thus applicable for testing materials prepared by the sol-gel method. (Z.M.)

  3. A Novel Method for Assessing Sex-Specific and Genotype-Specific Response to Injury in Astrocyte Culture

    Science.gov (United States)

    Liu, Mingyue; Oyarzabal, Esteban; Yang, Rui; Murphy, Stephanie J; Hurn, Patricia D.

    2008-01-01

    Female astrocytes sustain less cell death from oxygen-glucose deprivation (OGD) than male astrocytes. Arimidex, an aromatase inhibitor, abolishes these sex differences. To verify sex-dependent differences in P450 aromatase function in astrocyte cell death following OGD, we developed a novel method that uses sex-specific and genotype-specific single pup primary astrocyte cultures from wild-type (WT) and aromatase-knockout (ArKO) mice. After determining sex by external and internal examination as well as PCR and genotype by PCR amplification of tail cDNA, we established cultures from 1−3 day-old male and female, WT and ArKO mice pups and grew them to confluence in estrogen-free media. Cell death was measured by lactate dehydrogenase (LDH) assay. Our study shows that, while WT female astrocytes are more resistant to OGD than WT male cells, sex differences disappear in ArKO cells. Cell death is significantly increased in ArKO compared to WT in female astrocytes but not male cells. Therefore, P450 aromatase appears to be essential in endogenous neuroprotection in females, and this finding may have clinical implications. This innovative technique may also be applied to other in vitro studies of sex-related functional differences. PMID:18436308

  4. Method for Developing Descriptions of Hard-to-Price Products: Results of the Telecommunications Product Study

    Energy Technology Data Exchange (ETDEWEB)

    Conrad, F.; Tonn, B.

    1999-05-01

    This report presents the results of a study to test a new method for developing descriptions of hard-to-price products. The Bureau of Labor Statistics (BLS) is responsible for collecting data to estimate price indices such as the Consumers Price Index (BLS) is responsible for collecting data to estimate price indices such as the Consumers Price Index (CPI). BLS accomplishes this task by sending field staff to places of business to price actual products. The field staff are given product checklists to help them determine whether products found today are comparable to products priced the previous month. Prices for non-comparable products are not included in the current month's price index calculations. A serious problem facing BLS is developing product checklists for dynamic product areas, new industries, and the service sector. It is difficult to keep checklists up-to-date and quite often simply to develop checklists for service industry products. Some people estimates that upwards of 50 % of US economic activity is not accounted for in the CPI

  5. Production of Solar Cells in Space from Non Specific Ores by Utilization of Electronically Enhanced Sputtering

    Science.gov (United States)

    Curreri, Peter A.

    2009-01-01

    An ideal method of construction in space would utilize some form of the Universal Differentiator and Universal Constructor as described by Von Neumann (1). The Universal Differentiator is an idealized non ore specific extractive device which is capable of breaking any ore into its constituent elements, and the Universal Constructor can utilize these elements to build any device with controllability to the nanometer scale. During the Human Exploration Initiative program in the early 1990s a conceptual study was done (2) to understand whether such devices were feasible with near term technology for the utilization of space resources and energy. A candidate system was proposed which would utilize electronically enhanced sputtering as the differentiator. Highly ionized ions would be accelerated to a kinetic energy at which the interaction between them and the lattice elections in the ore would be at a maximum. Experiments have shown that the maximum disintegration of raw material occurs at an ion kinetic energy of about 5 MeV, regardless of the composition and structure of the raw material. Devices that could produce charged ion beams in this energy range in space were being tested in the early 1990s. At this energy, for example an ion in a beam of fluorine ions yields about 8 uranium ions from uranium fluoride, 1,400 hydrogen and oxygen atoms from ice, or 7,000 atoms from sulfur dioxide ice. The ions from the disintegrated ore would then be driven by an electrical field into a discriminator in the form of a mass spectrometer, where the magnetic field would divert the ions into collectors for future use or used directly in molecular beam construction techniques. The process would require 10-7 Torr vacuum which would be available in space or on the moon. If the process were used to make thin film silicon solar cells (ignoring any energy inefficiency for beam production), then energy break even for solar cells in space would occur after 14 days.

  6. Methods and materials for deconstruction of biomass for biofuels production

    Science.gov (United States)

    Schoeniger, Joseph S; Hadi, Masood Zia

    2015-05-05

    The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.

  7. Discrimination symbol applying method for sintered nuclear fuel product

    International Nuclear Information System (INIS)

    Ishizaki, Jin

    1998-01-01

    The present invention provides a symbol applying method for applying discrimination information such as an enrichment degree on the end face of a sintered nuclear product. Namely, discrimination symbols of information of powders are applied by a sintering aid to the end face of a molded member formed by molding nuclear fuel powders under pressure. Then, the molded product is sintered. The sintering aid comprises aluminum oxide, a mixture of aluminum oxide and silicon dioxide, aluminum hydride or aluminum stearate alone or in admixture. As an applying means of the sintering aid, discrimination symbols of information of powders are drawn by an isostearic acid on the end face of the molded product, and the sintering aid is sprayed thereto, or the sintering aid is applied directly, or the sintering aid is suspended in isostearic acid, and the suspension is applied with a brush. As a result, visible discrimination information can be applied to the sintered member easily. (N.H.)

  8. An Entry Point for Formal Methods: Specification and Analysis of Event Logs

    Directory of Open Access Journals (Sweden)

    Howard Barringer

    2010-03-01

    Full Text Available Formal specification languages have long languished, due to the grave scalability problems faced by complete verification methods. Runtime verification promises to use formal specifications to automate part of the more scalable art of testing, but has not been widely applied to real systems, and often falters due to the cost and complexity of instrumentation for online monitoring. In this paper we discuss work in progress to apply an event-based specification system to the logging mechanism of the Mars Science Laboratory mission at JPL. By focusing on log analysis, we exploit the "instrumentation" already implemented and required for communicating with the spacecraft. We argue that this work both shows a practical method for using formal specifications in testing and opens interesting research avenues, including a challenging specification learning problem.

  9. Modes in Component Behavior Specification via EBP and their Application in Product Lines

    Czech Academy of Sciences Publication Activity Database

    Kofroň, Jan; Plášil, František; Šerý, O.

    2009-01-01

    Roč. 51, č. 1 (2009), s. 31-41 ISSN 0950-5849 R&D Projects: GA AV ČR 1ET400300504 Grant - others:GA ČR(CZ) GA201/08/0266 Institutional research plan: CEZ:AV0Z10300504 Keywords : behavior specification * component modes * software product lines Subject RIV: JC - Computer Hardware ; Software Impact factor: 1.821, year: 2009

  10. The GLAS Standard Data Products Specification-Data Dictionary, Version 1.0. Volume 15

    Science.gov (United States)

    Lee, Jeffrey E.

    2013-01-01

    The Geoscience Laser Altimeter System (GLAS) is the primary instrument for the ICESat (Ice, Cloud and Land Elevation Satellite) laser altimetry mission. ICESat was the benchmark Earth Observing System (EOS) mission for measuring ice sheet mass balance, cloud and aerosol heights, as well as land topography and vegetation characteristics. From 2003 to 2009, the ICESat mission provided multi-year elevation data needed to determine ice sheet mass balance as well as cloud property information, especially for stratospheric clouds common over polar areas. It also provided topography and vegetation data around the globe, in addition to the polar-specific coverage over the Greenland and Antarctic ice sheets.This document contains the data dictionary for the GLAS standard data products. It details the parameters present on GLAS standard data products. Each parameter is defined with a short name, a long name, units on product, type of variable, a long description and products that contain it. The term standard data products refers to those EOS instrument data that are routinely generated for public distribution. These products are distributed by the National Snow and Ice Data Center (NSDIC).

  11. Calculations of pair production by Monte Carlo methods

    International Nuclear Information System (INIS)

    Bottcher, C.; Strayer, M.R.

    1991-01-01

    We describe some of the technical design issues associated with the production of particle-antiparticle pairs in very large accelerators. To answer these questions requires extensive calculation of Feynman diagrams, in effect multi-dimensional integrals, which we evaluate by Monte Carlo methods on a variety of supercomputers. We present some portable algorithms for generating random numbers on vector and parallel architecture machines. 12 refs., 14 figs

  12. Systems and methods for preparation and separation of products

    Science.gov (United States)

    Gilliam, Ryan J.; Boggs, Bryan; Self, Kyle; Leclerc, Margarete K.; Gorer, Alexander; Weiss, Michael J.; Miller, John H.; Mohanta, Samaresh

    2015-12-01

    There are provided methods and systems for an electrochemical cell including an anode and a cathode where the anode is contacted with a metal ion that converts the metal ion from a lower oxidation state to a higher oxidation state. The metal ion in the higher oxidation state is reacted with hydrogen gas, an unsaturated hydrocarbon, and/or a saturated hydrocarbon to form products.

  13. A procedure for the production of steel exhibiting a low specific activity of gamma emitters

    International Nuclear Information System (INIS)

    Dolenek, J.; Raska, P.; Kodrle, L. et al.

    1989-01-01

    Steel exhibiting low specific gamma activity can be obtained from a metallic charge containing liquid and solid pig iron produced from ores, sinters, coke, limestone and other components. This charge is worked up in a metallurgical fining unit using predetermined amounts of slag-forming substances such as lime, limestone and dolomite; fining ore can also be present. The smelt must be kept in constant motion. The pig iron smelt for the production of this steel contains 0.1-1.1% Si and 0.1-1.0% Mn. All equipment with which the charge and steel will come in contact must be free from remains of previous productions and, preferrably, fitted with new lining. This concerns runners, pig iron transportation mixers, ladles and the production unit. (P.A.)

  14. Production of Cyclin D1 specific siRNAs by double strand processing for gene therapy of esophageal squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Negar Mottaghi-Dastjerdi

    2013-02-01

    Conclusion: dsRNA digestion method includes several steps which the product of each step is used as the precursor for the next step. So optimization and increasing the specificity and product yield should be in the most important goals of the study, because the yield of each step has a direct relationship with the final product yield which is siRNA. Optimizing and increasing the yield, dsRNA digestion method could be a rapid, available and profitable method for siRNA generation, providing large amounts of siRNA.

  15. Determination of the thermal conductivity and specific heat capacity of neem seeds by inverse problem method

    Directory of Open Access Journals (Sweden)

    S.N. Nnamchi

    2010-01-01

    Full Text Available Determination of the thermal conductivity and the specific heat capacity of neem seeds (Azadirachta indica A. Juss usingthe inverse method is the main subject of this work. One-dimensional formulation of heat conduction problem in a spherewas used. Finite difference method was adopted for the solution of the heat conduction problem. The thermal conductivityand the specific heat capacity were determined by least square method in conjunction with Levenberg-Marquardt algorithm.The results obtained compare favourably with those obtained experimentally. These results are useful in the analysis ofneem seeds drying and leaching processes.

  16. Production of high-specific activity radionuclides using SM high-flux reactor

    International Nuclear Information System (INIS)

    Karelin, Ye.A.; Toporov, Yu.G.; Filimonov, V.T.; Vakhetov, F.Z.; Tarasov, V.A.; Kuznetsov, R.A.; Lebedev, V.M.; Andreev, O.I.; Melnik, M.I.; Gavrilov, V.D.

    1997-01-01

    The development of High Specific Activity (HSA) radionuclides production technologies is one of the directions of RIAR activity, and the high flux research reactor SM, having neutron flux density up to 2.10 15 cm -2 s 1 in a wide range of neutron spectra hardness, plays the principal role in this development. The use of a high-flux reactor for radionuclide production provides the following advantages: production of radionuclides with extremely high specific activity, decreasing of impurities content in irradiated targets (both radioactive and non-radioactive), cost-effective use of expensive isotopically enriched target materials. The production technologies of P-33, Gd-153, W-188, Ni-63, Fe-55,59, Sn-113,117m,119m, Sr- 89, applied in industry, nuclear medicine, research, etc, were developed by RIAR during the last 5-10 years. The research work included the development of calculation procedures for radionuclide reactor accumulation forecast, experimental determination of neutron cross-sections, the development of irradiated materials reprocessing procedures, isolation and purification of radionuclides. The principal results are reviewed in the paper. (authors)

  17. Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production

    Directory of Open Access Journals (Sweden)

    Zhou Xue-Rong

    2010-03-01

    Full Text Available Abstract Background Metabolic engineering of seed biosynthetic pathways to diversify and improve crop product quality is a highly active research area. The validation of genes driven by seed-specific promoters is time-consuming since the transformed plants must be grown to maturity before the gene function can be analysed. Results In this study we demonstrate that genes driven by seed-specific promoters contained within complex constructs can be transiently-expressed in the Nicotiana benthamiana leaf-assay system by co-infiltrating the Arabidopsis thaliana LEAFY COTYLEDON2 (LEC2 gene. A real-world case study is described in which we first assembled an efficient transgenic DHA synthesis pathway using a traditional N. benthamiana Cauliflower Mosaic Virus (CaMV 35S-driven leaf assay before using the LEC2-extended assay to rapidly validate a complex seed-specific construct containing the same genes before stable transformation in Arabidopsis. Conclusions The LEC2-extended N. benthamiana assay allows the transient activation of seed-specific promoters in leaf tissue. In this study we have used the assay as a rapid preliminary screen of a complex seed-specific transgenic construct prior to stable transformation, a feature that will become increasingly useful as genetic engineering moves from the manipulation of single genes to the engineering of complex pathways. We propose that the assay will prove useful for other applications wherein rapid expression of transgenes driven by seed-specific constructs in leaf tissue are sought.

  18. Rapid production of antigen-specific monoclonal antibodies from a variety of animals

    Directory of Open Access Journals (Sweden)

    Kurosawa Nobuyuki

    2012-09-01

    Full Text Available Abstract Background Although a variety of animals have been used to produce polyclonal antibodies against antigens, the production of antigen-specific monoclonal antibodies from animals remains challenging. Results We propose a simple and rapid strategy to produce monoclonal antibodies from a variety of animals. By staining lymph node cells with an antibody against immunoglobulin and a fluorescent dye specific for the endoplasmic reticulum, plasma/plasmablast cells were identified without using a series of antibodies against lineage markers. By using a fluorescently labeled antigen as a tag for a complementary cell surface immunoglobulin, antigen-specific plasma/plasmablast cells were sorted from the rest of the cell population by fluorescence-activated cell sorting. Amplification of cognate pairs of immunoglobulin heavy and light chain genes followed by DNA transfection into 293FT cells resulted in the highly efficient production of antigen-specific monoclonal antibodies from a variety of immunized animals. Conclusions Our technology eliminates the need for both cell propagation and screening processes, offering a significant advantage over hybridoma and display strategies.

  19. Estimation of the specific activity of radioiodinated gonadotrophins: comparison of three methods

    Energy Technology Data Exchange (ETDEWEB)

    Englebienne, P [Centre for Research and Diagnosis in Endocrinology, Kain (Belgium); Slegers, G [Akademisch Ziekenhuis, Ghent (Belgium). Lab. voor Analytische Chemie

    1983-01-14

    The authors compared 3 methods for estimating the specific activity of radioiodinated gonadotrophins. Two of the methods (column recovery and isotopic dilution) gave similar results, while the third (autodisplacement) gave significantly higher estimations. In the autodisplacement method, B/T ratios, obtained when either labelled hormone alone, or labelled and unlabelled hormone, are added to the antibody, were compared as estimates of the mass of hormone iodinated. It is likely that immunologically unreactive impurities present in the labelled hormone solution invalidate such comparison.

  20. NEW MEAT PRODUCTS WITH IMMUNOMODULATORY EFFECT CREATION METHOD

    Directory of Open Access Journals (Sweden)

    I. V. Kaltovich

    2016-01-01

    Full Text Available New meat products with immunomodulatory effect creation method reflecting differential characteristics of technological stages of manufacture of those types of meat products, including issues on the selection of primary and secondary raw materials, guidelines for development of formulations and production technologies, legislative requirements towards its labeling, etc, has been developed for the first time. A list of prospective meat raw materials for the manufacture of products with immunomodulatory effect was established: beef, pork, rabbit meat, broiler chicken meat, turkey, veal, ostrich meat, which have high content of protein (14,3– 21,7%, low content of fat (1,2–16,1%, excluding pork (33,3%, high levels of minimum amino-acid score (90,0–104,0%, protein quality indicator(0,91–1,64, essential amino acid index (1,16-1,25, coefficient of utility of amino acid content (0,72–0,86 and close to optimum fatty acid content, and also contain a great number of vitamins and minerals which play a significant role for immunity improvement. It was determined that the following functional ingredients are recommended to use: amino acids (valine, leucine, isoleucine, methionine, threonine, arginine, tryptophan, lysine, histidin, phenylalanyl, vitamins and provitamins (C,E, beta-carotene, B vitamins(Bc, B12, PP, etc., P(bioflavonoid complex, H, K, minerals (calcium, magnesium, iron, cuprum, zinc, manganese, selenium, polyunsaturated fatty acids omega-3 and omega-6, pseudo-vitamins (L-carnitin, coenzyme Q10, polysaccharides and peptides naturally occurring(squalen, B-Carotene, ginger, shiitake mushrooms, probiotics and prebiotics, glutathione, indole and lycopienes, bioflavonoids, L-arginine, N-acetylcysteine, gel from seaweed «Lamifaren». The use of the developed meat products with immunomodulatory effect creation method by process engineers of meat processing factories will allow them to form a single scientifically grounded approach during the

  1. Interlaboratory diagnostic accuracy of a Salmonella specific PCR-based method

    DEFF Research Database (Denmark)

    Malorny, B.; Hoorfar, Jeffrey; Hugas, M.

    2003-01-01

    A collaborative study involving four European laboratories was conducted to investigate the diagnostic accuracy of a Salmonella specific PCR-based method, which was evaluated within the European FOOD-PCR project (http://www.pcr.dk). Each laboratory analysed by the PCR a set of independent obtained...... presumably naturally contaminated samples and compared the results with the microbiological culture method. The PCR-based method comprised a preenrichment step in buffered peptone water followed by a thermal cell lysis using a closed tube resin-based method. Artificially contaminated minced beef and whole......-based diagnostic methods and is currently proposed as international standard document....

  2. Applying Human-Centered Design Methods to Scientific Communication Products

    Science.gov (United States)

    Burkett, E. R.; Jayanty, N. K.; DeGroot, R. M.

    2016-12-01

    Knowing your users is a critical part of developing anything to be used or experienced by a human being. User interviews, journey maps, and personas are all techniques commonly employed in human-centered design practices because they have proven effective for informing the design of products and services that meet the needs of users. Many non-designers are unaware of the usefulness of personas and journey maps. Scientists who are interested in developing more effective products and communication can adopt and employ user-centered design approaches to better reach intended audiences. Journey mapping is a qualitative data-collection method that captures the story of a user's experience over time as related to the situation or product that requires development or improvement. Journey maps help define user expectations, where they are coming from, what they want to achieve, what questions they have, their challenges, and the gaps and opportunities that can be addressed by designing for them. A persona is a tool used to describe the goals and behavioral patterns of a subset of potential users or customers. The persona is a qualitative data model that takes the form of a character profile, built upon data about the behaviors and needs of multiple users. Gathering data directly from users avoids the risk of basing models on assumptions, which are often limited by misconceptions or gaps in understanding. Journey maps and user interviews together provide the data necessary to build the composite character that is the persona. Because a persona models the behaviors and needs of the target audience, it can then be used to make informed product design decisions. We share the methods and advantages of developing and using personas and journey maps to create more effective science communication products.

  3. Using a fuzzy comprehensive evaluation method to determine product usability: A test case.

    Science.gov (United States)

    Zhou, Ronggang; Chan, Alan H S

    2017-01-01

    In order to take into account the inherent uncertainties during product usability evaluation, Zhou and Chan [1] proposed a comprehensive method of usability evaluation for products by combining the analytic hierarchy process (AHP) and fuzzy evaluation methods for synthesizing performance data and subjective response data. This method was designed to provide an integrated framework combining the inevitable vague judgments from the multiple stages of the product evaluation process. In order to illustrate the effectiveness of the model, this study used a summative usability test case to assess the application and strength of the general fuzzy usability framework. To test the proposed fuzzy usability evaluation framework [1], a standard summative usability test was conducted to benchmark the overall usability of a specific network management software. Based on the test data, the fuzzy method was applied to incorporate both the usability scores and uncertainties involved in the multiple components of the evaluation. Then, with Monte Carlo simulation procedures, confidence intervals were used to compare the reliabilities among the fuzzy approach and two typical conventional methods combining metrics based on percentages. This case study showed that the fuzzy evaluation technique can be applied successfully for combining summative usability testing data to achieve an overall usability quality for the network software evaluated. Greater differences of confidence interval widths between the method of averaging equally percentage and weighted evaluation method, including the method of weighted percentage averages, verified the strength of the fuzzy method.

  4. A method of refining aromatic hydrocarbons from coal chemical production

    Energy Technology Data Exchange (ETDEWEB)

    Zieborak, K.; Koprowski, A.; Ratajczak, W.

    1979-10-01

    A method is disclosed for refining aromatic hydrocarbons of coal chemical production by contact of liquid aromatic hydrocarbons and their mixtures with a strongly acid macroporous sulfocationite in the H-form at atmospheric pressure and high temperature. The method is distinguished in that the aromatic hydrocarbons and their mixtures, from which alkali compounds have already been removed, are supplied for refinement with the sulfocationite with simultaneous addition of olefin derivatives of aromatic hydrocarbons, followed by separation of pure hydrocarbons by rectification. Styrene or alpha-methylstyrene is used as the olefin derivatives of the aromatic hydrocarbons. The method is performed in several stages with addition of olefin derivatives of aromatic hydrocarbons at each stage.

  5. Participatory methods for initiating manufacturing employees' involvement in product innovation

    DEFF Research Database (Denmark)

    Jensen, Anna Rose Vagn; Jensen, Christian Schou; Broberg, Ole

    2016-01-01

    approach that we apply to two case companies, this paper presents an empirical study of how to initiate involvement of manufacturing employees in R&D activities. We have used participatory methods from design thinking that has the ability to create relations between employees from different backgrounds......Employee-driven innovation has the potential to improve product innovation by involving employees as innovative resources. However, it can be a challenge to turn the potential into a reality of collaboration practices across organizational structures and culture. Through an interactive research...... and through a series of facilitated workshops we have investigated how these methods can initiate employee involvement. We see that participatory methods can improve understanding and relation between R&D and manufacturing departments, and thereby support a creative collaboration and emergence of employee...

  6. Study of the Assessment Method for N Excretion in Sustainable Heavy Pigs Production

    Science.gov (United States)

    Guo, Kaijun; Liu, Fenghua; Xu, Xiaolong; Xu, Jianqin; Zoccarato, Ivo

    Italian heavy pigs, with an average slaughtering body weight of 150-170 kg, are world-wide famous for its Parma ham production. Because the requirement of market diversity, producers are interested in ham production following the procedure of Italian pork industry. However, with ever growing public concern about nitrogen (N) pollution in the environment, it is necessary to determine a suitable method to measure N excretion from heavy pig production. The N retention was calculated by factorial method and compared with estimations of other methods available in literature. The results showed that the N percentage of heavy pigs is 2.43% ± 0.07% on body weight basis and the percentage of N excretion was approximately 69.62% ± 0.20 of N intake. Regarding the N excretion of estimation methods, the proposal of Xiccato et al. was closer to reality of the heavy pig production than other methods and could be used as a standard way to calculate the N excretion. Besides the overall standard, it is opportune to make a N balance sheet for every individual farm under specific conditions. Only in this way, the farmers can realize their deficiencies and will voluntarily follow the Good Management Practice (GMP) indications so as to guarantee a sustainable development of pig production.

  7. Specific light uptake rates can enhance astaxanthin productivity in Haematococcus lacustris.

    Science.gov (United States)

    Lee, Ho-Sang; Kim, Z-Hun; Park, Hanwool; Lee, Choul-Gyun

    2016-05-01

    Lumostatic operation was applied for efficient astaxanthin production in autotrophic Haematococcus lacustris cultures using 0.4-L bubble column photobioreactors. The lumostatic operation in this study was performed with three different specific light uptake rates (q(e)) based on cell concentration, cell projection area, and fresh weight as one-, two- and three-dimensional characteristics values, respectively. The q(e) value from the cell concentration (q(e1D)) obtained was 13.5 × 10⁻⁸ μE cell⁻¹ s⁻¹, and the maximum astaxanthin concentration was increased to 150 % compared to that of a control with constant light intensity. The other optimum q e values by cell projection area (q(e2D)) and fresh weight (q( e3D)) were determined to be 195 μE m⁻² s⁻¹ and 10.5 μE g⁻¹ s⁻¹ for astaxanthin production, respectively. The maximum astaxanthin production from the lumostatic cultures using the parameters controlled by cell projection area (2D) and fresh weight (3D) also increased by 36 and 22% over that of the controls, respectively. When comparing the optimal q e values among the three different types, the lumostatic cultures using q(e) based on fresh weight showed the highest astaxanthin productivity (22.8 mg L⁻¹ day⁻¹), which was a higher level than previously reported. The lumostatic operations reported here demonstrated that more efficient and effective astaxanthin production was obtained by H. lacustris than providing a constant light intensity, regardless of which parameter is used to calculate the specific light uptake rate.

  8. Weed control by direct injection of plant protection products according to specific situations

    Directory of Open Access Journals (Sweden)

    Krebs, Mathias

    2016-02-01

    Full Text Available Precision Farming in agriculture allows a site-specific management of the crop. The aim of plant protection is to apply plant protection products (PPP according to the site specific requirements on the field. Within the context of a research program to promote innovation, a sprayer with direct injection of plant protection products was developed. The direct injection offers site specific spraying of different individual PPP in a single pass. The sprayer prototype is equipped with a special spray boom combining three nozzle lines. In order to prevent delay times, the nozzle lines are preloaded before spraying. First results for weed control from test stand measurements and field trials showed that the injection pumps work with high accuracy. The prototype can be used without delay times site specific with up to three different herbicides. Field trials for site-specific weed control in winter wheat demonstrate the applicability of the system under practical conditions. By treatment of subareas herbicides and therefore costs could be saved. A reduction in yield compared with the conventionally treated field areas could not be ascertained. Also an efficacy reduction through washout of active ingredient from target surfaces due to simultaneous use of all three nozzle lines with up to 1050 l/ha application rate could not be detected. At high water spray rates, the efficacy effect occurs delayed. Overall, the newly developed direct injection system proved fieldabillity during the first tests. So weed control can be carried out situation-responsive, which can save herbicides and environmental impacts are reduced.

  9. Analysis of Specific Features of the Ukrainian Market of Natural Gas Production and Consumption

    Directory of Open Access Journals (Sweden)

    Lelyuk Oleksiy V.

    2013-11-01

    Full Text Available The article provides results of the study of specific features of the Ukrainian market of natural gas production and consumption. It analyses dynamics of the specific weight of Ukraine in general volumes of natural gas consumption in the world, dynamics of natural gas consumption in Ukraine during 1990 – 2012 and dependence of natural gas consumption on GDP volumes by the purchasing power parity. It studies the structure of natural gas consumption by regions in 2012 and sectors of economy, resource base of natural gas in Ukraine and also dynamics of established resources of natural gas in Ukraine and dynamics of natural gas production. It analyses base rates of growth of natural gas resources and production in Ukraine. It considers dynamics of import of natural gas into Ukraine and its import prices and also the structure of natural gas import. It identifies the balance of the natural gas market in Ukraine. On the basis of the conducted analysis the article proves that Ukraine is a gas-deficit country of the world, which depends on natural gas import supplies.

  10. Alternative methods of modeling wind generation using production costing models

    International Nuclear Information System (INIS)

    Milligan, M.R.; Pang, C.K.

    1996-08-01

    This paper examines the methods of incorporating wind generation in two production costing models: one is a load duration curve (LDC) based model and the other is a chronological-based model. These two models were used to evaluate the impacts of wind generation on two utility systems using actual collected wind data at two locations with high potential for wind generation. The results are sensitive to the selected wind data and the level of benefits of wind generation is sensitive to the load forecast. The total production cost over a year obtained by the chronological approach does not differ significantly from that of the LDC approach, though the chronological commitment of units is more realistic and more accurate. Chronological models provide the capability of answering important questions about wind resources which are difficult or impossible to address with LDC models

  11. Method for creating high carbon content products from biomass oil

    Science.gov (United States)

    Parker, Reginald; Seames, Wayne

    2012-12-18

    In a method for producing high carbon content products from biomass, a biomass oil is added to a cracking reactor vessel. The biomass oil is heated to a temperature ranging from about 100.degree. C. to about 800.degree. C. at a pressure ranging from about vacuum conditions to about 20,700 kPa for a time sufficient to crack the biomass oil. Tar is separated from the cracked biomass oil. The tar is heated to a temperature ranging from about 200.degree. C. to about 1500.degree. C. at a pressure ranging from about vacuum conditions to about 20,700 kPa for a time sufficient to reduce the tar to a high carbon content product containing at least about 50% carbon by weight.

  12. Comparative Assessment of Advanced Gay Hydrate Production Methods

    Energy Technology Data Exchange (ETDEWEB)

    M. D. White; B. P. McGrail; S. K. Wurstner

    2009-06-30

    Displacing natural gas and petroleum with carbon dioxide is a proven technology for producing conventional geologic hydrocarbon reservoirs, and producing additional yields from abandoned or partially produced petroleum reservoirs. Extending this concept to natural gas hydrate production offers the potential to enhance gas hydrate recovery with concomitant permanent geologic sequestration. Numerical simulation was used to assess a suite of carbon dioxide injection techniques for producing gas hydrates from a variety of geologic deposit types. Secondary hydrate formation was found to inhibit contact of the injected CO{sub 2} regardless of injectate phase state, thus diminishing the exchange rate due to pore clogging and hydrate zone bypass of the injected fluids. Additional work is needed to develop methods of artificially introducing high-permeability pathways in gas hydrate zones if injection of CO{sub 2} in either gas, liquid, or micro-emulsion form is to be more effective in enhancing gas hydrate production rates.

  13. Structure-guided mutational analysis reveals the functional requirements for product specificity of DOT1 enzymes.

    Science.gov (United States)

    Dindar, Gülcin; Anger, Andreas M; Mehlhorn, Christine; Hake, Sandra B; Janzen, Christian J

    2014-11-12

    DOT1 enzymes are conserved methyltransferases that catalyse the methylation of lysine 79 on histone H3 (H3K79). Most eukaryotes contain one DOT1 enzyme, whereas African trypanosomes have two homologues, DOT1A and DOT1B, with different enzymatic activities. DOT1A mediates mono- and dimethylation of H3K76, the homologue of H3K79 in other organisms, whereas DOT1B additionally catalyses H3K76 trimethylation. However, it is unclear how these different enzymatic activities are achieved. Here we employ a trypanosomal nucleosome reconstitution system and structure-guided homology modelling to identify critical residues within and outside the catalytic centre that modulate product specificity. Exchange of these residues transfers the product specificity from one enzyme to the other, and reveals the existence of distinct regulatory domains adjacent to the catalytic centre. Our study provides the first evidence that a few crucial residues in DOT1 enzymes are sufficient to catalyse methyl-state-specific reactions. These results might also have far-reaching consequences for the functional understanding of homologous enzymes in higher eukaryotes.

  14. A root specific induction of carotenoid biosynthesis contributes to ABA production upon salt stress in arabidopsis.

    Directory of Open Access Journals (Sweden)

    M Águila Ruiz-Sola

    Full Text Available Abscisic acid (ABA is a hormone that plays a vital role in mediating abiotic stress responses in plants. Salt exposure induces the synthesis of ABA through the cleavage of carotenoid precursors (xanthophylls, which are found at very low levels in roots. Here we show that de novo ABA biosynthesis in salt-treated Arabidopsis thaliana roots involves an organ-specific induction of the carotenoid biosynthetic pathway. Upregulation of the genes encoding phytoene synthase (PSY and other enzymes of the pathway producing ABA precursors was observed in roots but not in shoots after salt exposure. A pharmacological block of the carotenoid pathway substantially reduced ABA levels in stressed roots, confirming that an increase in carotenoid accumulation contributes to fuel hormone production after salt exposure. Treatment with exogenous ABA was also found to upregulate PSY expression only in roots, suggesting an organ-specific feedback regulation of the carotenoid pathway by ABA. Taken together, our results show that the presence of high concentrations of salt in the growth medium rapidly triggers a root-specific activation of the carotenoid pathway, probably to ensure a proper supply of ABA precursors required for a sustained production of the hormone.

  15. Chapter 16: Retrocommissioning Evaluation Protocol. The Uniform Methods Project: Methods for Determining Energy Efficiency Savings for Specific Measures

    Energy Technology Data Exchange (ETDEWEB)

    Kurnik, Charles W. [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Tiessen, Alex [Posterity Group, Derwood, MD (United States)

    2017-10-09

    Retrocommissioning (RCx) is a systematic process for optimizing energy performance in existing buildings. It specifically focuses on improving the control of energy-using equipment (e.g., heating, ventilation, and air conditioning [HVAC] equipment and lighting) and typically does not involve equipment replacement. Field results have shown proper RCx can achieve energy savings ranging from 5 percent to 20 percent, with a typical payback of two years or less (Thorne 2003). The method presented in this protocol provides direction regarding: (1) how to account for each measure's specific characteristics and (2) how to choose the most appropriate savings verification approach.

  16. Screening DNA chip and event-specific multiplex PCR detection methods for biotech crops.

    Science.gov (United States)

    Lee, Seong-Hun

    2014-11-01

    There are about 80 biotech crop events that have been approved by safety assessment in Korea. They have been controlled by genetically modified organism (GMO) and living modified organism (LMO) labeling systems. The DNA-based detection method has been used as an efficient scientific management tool. Recently, the multiplex polymerase chain reaction (PCR) and DNA chip have been developed as simultaneous detection methods for several biotech crops' events. The event-specific multiplex PCR method was developed to detect five biotech maize events: MIR604, Event 3272, LY 038, MON 88017 and DAS-59122-7. The specificity was confirmed and the sensitivity was 0.5%. The screening DNA chip was developed from four endogenous genes of soybean, maize, cotton and canola respectively along with two regulatory elements and seven genes: P35S, tNOS, pat, bar, epsps1, epsps2, pmi, cry1Ac and cry3B. The specificity was confirmed and the sensitivity was 0.5% for four crops' 12 events: one soybean, six maize, three cotton and two canola events. The multiplex PCR and DNA chip can be available for screening, gene-specific and event-specific analysis of biotech crops as efficient detection methods by saving on workload and time. © 2014 Society of Chemical Industry. © 2014 Society of Chemical Industry.

  17. A novel method for fission product noble gas sampling

    International Nuclear Information System (INIS)

    Jain, S.K.; Prakash, Vivek; Singh, G.K.; Vinay, Kr.; Awsthi, A.; Bihari, K.; Joyson, R.; Manu, K.; Gupta, Ashok

    2008-01-01

    Noble gases occur to some extent in the Earth's atmosphere, but the concentrations of all but argon are exceedingly low. Argon is plentiful, constituting almost 1 % of the air. Fission Product Noble Gases (FPNG) are produced by nuclear fission and large parts of FPNG is produced in Nuclear reactions. FPNG are b-j emitters and contributing significantly in public dose. During normal operation of reactor release of FPNG is negligible but its release increases in case of fuel failure. Xenon, a member of FPNG family helps in identification of fuel failure and its extent in PHWRs. Due to above reasons it becomes necessary to assess the FPNG release during operation of NPPs. Presently used methodology of assessment of FPNG, at almost all power stations is Computer based gamma ray spectrometry. This provides fission product Noble gases nuclide identification through peak search of spectra. The air sample for the same is collected by grab sampling method, which has inherent disadvantages. An alternate method was developed at Rajasthan Atomic Power Station (RAPS) - 3 and 4 for assessment of FPNG, which uses adsorption phenomena for collection of air samples. This report presents details of sampling method for FPNG and noble gases in different systems of Nuclear Power Plant. (author)

  18. Continued SOFC cell and stack technology and improved production methods

    Energy Technology Data Exchange (ETDEWEB)

    Wandel, M.; Brodersen, K.; Phair, J. (and others)

    2009-05-15

    Within this project significant results are obtained on a number of very diverse areas ranging from development of cell production, metallic creep in interconnect to assembling and test of stacks with foot print larger than 500 cm2. Out of 38 milestones 28 have been fulfilled and 10 have been partly fulfilled. This project has focused on three main areas: 1) The continued cell development and optimization of manufacturing processes aiming at production of large foot-print cells, improving cell performance and development environmentally more benign production methods. 2) Stack technology - especially stacks with large foot print and improving the stack design with respect to flow geometry and gas leakages. 3) Development of stack components with emphasis on sealing (for 2G as well as 3G), interconnect (coat, architecture and creep) and test development. Production of cells with a foot print larger than 500 cm2 is very difficult due to the brittleness of the cells and great effort has been put into this topic. Eight cells were successfully produced making it possible to assemble and test a real stack thereby giving valuable results on the prospects of stacks with large foot print. However, the yield rate is very low and a significant development to increase this yield lies ahead. Several lessons were learned on the stack level regarding 'large foot print' stacks. Modelling studies showed that the width of the cell primarily is limited by production and handling of the cell whereas the length (in the flow direction) is limited by e.g. pressure drop and necessary manifolding. The optimal cell size in the flow direction was calculated to be between approx20 cm and < 30 cm. From an economical point of view the production yield is crucial and stacks with large foot print cell area are only feasible if the cell production yield is significantly enhanced. Co-casting has been pursued as a production technique due to the possibilities in large scale production

  19. A method to determine site-specific, anisotropic fracture toughness in biological materials

    International Nuclear Information System (INIS)

    Bechtle, Sabine; Özcoban, Hüseyin; Yilmaz, Ezgi D.; Fett, Theo; Rizzi, Gabriele; Lilleodden, Erica T.; Huber, Norbert; Schreyer, Andreas; Swain, Michael V.; Schneider, Gerold A.

    2012-01-01

    Many biological materials are hierarchically structured, with highly anisotropic structures and properties on several length scales. To characterize the mechanical properties of such materials, detailed testing methods are required that allow precise and site-specific measurements on several length scales. We propose a fracture toughness measurement technique based on notched focused ion beam prepared cantilevers of lower and medium micron size scales. Using this approach, site-specific fracture toughness values in dental enamel were determined. The usefulness and challenges of the method are discussed.

  20. [Development and validation of event-specific quantitative PCR method for genetically modified maize LY038].

    Science.gov (United States)

    Mano, Junichi; Masubuchi, Tomoko; Hatano, Shuko; Futo, Satoshi; Koiwa, Tomohiro; Minegishi, Yasutaka; Noguchi, Akio; Kondo, Kazunari; Akiyama, Hiroshi; Teshima, Reiko; Kurashima, Takeyo; Takabatake, Reona; Kitta, Kazumi

    2013-01-01

    In this article, we report a novel real-time PCR-based analytical method for quantitation of the GM maize event LY038. We designed LY038-specific and maize endogenous reference DNA-specific PCR amplifications. After confirming the specificity and linearity of the LY038-specific PCR amplification, we determined the conversion factor required to calculate the weight-based content of GM organism (GMO) in a multilaboratory evaluation. Finally, in order to validate the developed method, an interlaboratory collaborative trial according to the internationally harmonized guidelines was performed with blind DNA samples containing LY038 at the mixing levels of 0, 0.5, 1.0, 5.0 and 10.0%. The precision of the method was evaluated as the RSD of reproducibility (RSDR), and the values obtained were all less than 25%. The limit of quantitation of the method was judged to be 0.5% based on the definition of ISO 24276 guideline. The results from the collaborative trial suggested that the developed quantitative method would be suitable for practical testing of LY038 maize.

  1. A production method for Cr-51 at IEN's cyclotron

    Energy Technology Data Exchange (ETDEWEB)

    Bastos, M A.V.; Britto, J L.Q. de; Vinagre, U M; Silva, A.G. da [Universidade Federal, Rio de Janeiro, RJ (Brazil). Coordenacao dos Programas de Pos-graduacao de Engenharia

    1990-01-01

    Thick target yields for the reactions {sup 51} V(p,n){sup 51} Cr and {sup 51} V(d, 2 n){sup 51} Cr were measured and compared with literature values. The excitation function for the {sup 51} V(p,n){sup 51} Cr reaction was also measured. A method for the production and separation of this radioisotope was developed via the {sup 51} V(p,n){sup 51} Cr reaction and a combination of Fe+3 coprecipitation and an anion exchange technique using a Dowex 1X8(100-200 mesh) resin in hydrochloric acid medium. (author).

  2. A production method for Cr-51 at IEN's cyclotron

    Energy Technology Data Exchange (ETDEWEB)

    Bastos, M A.V.; Britto, J L.Q. de; Vinagre, U M; Silva, A.G. da [Instituto de Engenharia Nuclear, Rio de Janeiro (Brazil). Dept. de Fisica

    1990-01-01

    Thick target yields for the reactions {sup 51}V(p,n){sup 51}Cr and {sup 51}V(d,2n){sup 51}Cr were measured and compared with literature values. The excitation function for the {sup 51}V(p,n){sup 51}Cr reaction was also measured. A method for the production and separation of this radioisotope was developed via the {sup 51}V(p,n){sup 51}Cr reaction and a combination of Fe{sup +3} coprecipitation and an anion exchange technique using a Dowex 1X8 (100-200 mesh) resin in hydrochloric acid medium. (orig.).

  3. Method of T-products in polaron theory

    International Nuclear Information System (INIS)

    Bogolubov, N.N. Jr.; Kurbatov, A.M.; Kireev, A.N.

    1985-11-01

    T-products method is used for the investigation of equilibrium thermodynamic properties of Frohlich's model in polaron theory. Polaron free energy at finite temperatures is calculated on the basis of Bogolubov's variational principle. A trial function is chosen in the most general form corresponding to arbitrary number of oscillators harmonically interacting with electron. The upper bound to the polaron ground state energy in limiting case of weak interaction and low temperatures is obtained and investigated in detail. It is shown that the result becomes more exact by increasing the number of oscillators. (author)

  4. A production method for Cr-51 at IEN's cyclotron

    International Nuclear Information System (INIS)

    Bastos, M.A.V.; Britto, J.L.Q. de; Vinagre, U.M.; Silva, A.G. da.

    1989-01-01

    Thick target yields for the reactions 51 V(p,n) 51 Cr and 51 V(d,2n) 51 Cr were measured and compared with literature values. The excitation function for the 51 V(p,n) 51 Cr reaction was also measured. A method for the production and separation of this radioisotope was developed via the 51 V(p,n) 51 Cr reaction and a combination of Fe +3 co-precipitation and an anion exchange technique using a Dowex 1x8(100-200 mesh) resin in hydrochloric acid medium [pt

  5. PAPER PRINTED PHOTOVOLTAIC CELLS: EMERGING METHOD OF PV CELL PRODUCTION

    OpenAIRE

    Nikhil S. Mane*, Avinash M. Patil2, Vishal P. Patil3

    2017-01-01

    An Solar energy is a renewable method for the energy production. The use of solar energy is increasing day by day and share of solar energy is increasing in the power sector of India. But as per pollution increases with energy consumption the need of solar energy will goes on increase in recent future as solar energy is a best option in both thermal and photovoltaic energy conversion processes. Photovoltaic cells are compact and has no movable parts which provides them effectiveness and easy ...

  6. Challenges in balancing the amount of solution information in requirement specifications for embedded products

    DEFF Research Database (Denmark)

    Savolainen, Juha; Hauksdóttir, Dagný; Mannion, Mike

    2013-01-01

    customers have such intimate knowledge of their products that their requirements tend to be very specific, and if the customer knows the exact solution needed that naturally will reduce the cost of the requirements elicitation as well as design activities. Practitioners are challenged to understand when......Requirements are traditionally viewed as being free of the details of an envisioned solution and specified using purely problem domain entities. Preventing premature design in the requirements permits the available design space not to be restricted too early which might inhibit innovative designs....... In practice, on many industrial projects, separating the problem and solution domain entities can be difficult, and arguably there are benefits for not doing so. Many customers feel more confident describing their requirements, often as the difference between the existing products and their needs, some...

  7. CONSIDERATIONS REGARDING THE DESIGN OF SPECIFIC TOURISM PRODUCTS FOR THE TOURISM DESTINATION ROMANIA

    Directory of Open Access Journals (Sweden)

    MONICA PAULA RAŢIU

    2011-01-01

    Full Text Available Romania, as tourism destination with its component micro-destinations (Muntenia, Oltenia, Banat - Crişana, Transylvania, Bucovina, Maramureş, Moldavia and Dobrogea, possesses many tourism attractions and at the same time has a real tourism potential. The fact that it is not included among the Europe’s „valuable” destinations represents a loss both for its inhabitants and for Europeans in general, too. One of the reasons is the lack of an image consolidated by identity elements or / and simply the lack of image that would represent the starting point for development of appropriate tourism products (developing a strategy. This paper proposes, starting from the image perceived by the inhabitants about their own destination – based on the example of Transylvania – to emphasize the importance and also the role of the destination image in developing the main directions of actions and, especially, in developing the strategy for the specific tourism products.

  8. Reticle inspection equipment productivity increase using SEMI specification for reticle and pod management

    Science.gov (United States)

    Taylor, Ron; Downey, Jack; Wood, Jeffrey; Lin, Yen-Hung; Bugata, Bharathi; Fan, Dongsheng; Hess, Carl; Wylie, Mark

    2016-10-01

    In this work, the SEMI specification for reticle and pod management (E109) with internal reticle library support has been integrated for the first time on KLA-Tencor's TeronTM and TeraScanTM reticle inspection tools. Manufacturing Execution System scheduling reticle jobs and Automated Material Handling System scheduling to transfer pods simultaneously have also been integrated and tested. GLOBALFOUNDRIES collaboratively worked with KLA-Tencor to successfully implement these capabilities. Both library and non-library scenarios have been demonstrated for comparison in a real production environment resulting in productivity increase of approximately 29% by making use of the library. Reticle re-qualification test cases were used for the comparison in this work.

  9. Economics of production of biogas from specifically-grown plant material. [New Zealand

    Energy Technology Data Exchange (ETDEWEB)

    Stewart, D. J.

    1977-10-15

    The production of biogas from plant materials is technologically very simple, and is the only process currently available (other than direct burning) for conversion of cellulose materials into energy or fuels that is feasible at a farm-scale, or even a home-scale, as well as a large industrial plant scale. For this reason the economics of biogas production can be considered at the farm-scale as well as the industrial scale. An accurate assessment of the economics at the farm-scale is possible, because commercially produced units are now available in New Zealand and in operation. However, although large-scale plants have been proposed and costed in the USA for the conversion of the cellulose component of garbage into biogas, operational data are not yet available, and the costing has not been applied to the use of specifically-grown plant material. Nevertheless, the large-scale plants envisaged use a large number of digesters each of 100,000 gallons capacity and can thus be regarded as a combination of farm-size units, although with some economics in digester size, number of pumps required, etc. For these reasons, this review of the economics of biogas production is based on the operation of commercial 20,000 gallon digesters available in NZ for farm-scale use. Factors governing the economics of farm-scale and industrial-scale production of biogas will be discussed in section 6.

  10. Ethanol production from the seaweed Gelidium amansii, using specific sugar acclimated yeasts.

    Science.gov (United States)

    Cho, Hyeyoung; Ra, Chae-Hun; Kim, Sung-Koo

    2014-02-28

    For the production of ethanol from seaweed as the source material, thermal acid hydrolysis and enzymatic saccharification were carried out for monosugars production of 25.5 g/l galactose and 7.6 g/l glucose using Gelidium amansii. The fermentation was performed with Pichia stipitis KCTC 7228 or Saccharomyces cerevisiae KCCM 1129. When wild P. stipitis and S. cerevisiae were used, the ethanol productions of 11.2 g/l and 6.9 g/l were produced, respectively. The ethanol productions of 16.6 g/l and 14.6 g/l were produced using P. stipitis and S. cerevisiae acclimated to high concentration of galactose, respectively. The yields of ethanol fermentation increased to 0.5 and 0.44 from 0.34 and 0.21 using acclimated P. stipitis and S. cerevisiae, respectively. Therefore, acclimation of yeasts to a specific sugar such as galactose reduced the glucose-induced repression on the transport of galactose.

  11. The impact of instrument-specific musical training on rhythm perception and production

    Directory of Open Access Journals (Sweden)

    Tomas Edward Matthews

    2016-02-01

    Full Text Available Various studies have shown that musical training can improve rhythmic perception and production. These findings tell us that music training can result in rhythm processing advantages but they do not tell us whether practicing a particular instrument could lead to specific effects on rhythm perception or production. The current study used a battery of four rhythm perception and production tasks that were designed to test both higher- and lower-level aspects of rhythm processing. Four groups of musicians (drummers, singers, pianists, string players and a control group of non-musicians were tested. Within-task differences in performance showed that factors such as meter, metrical complexity, tempo and beat phase significantly affected the ability to perceive and synchronize taps to a rhythm or beat. Musicians showed better performance on all rhythm tasks compared to non-musicians. Interestingly, our results revealed no significant differences between musician groups for the vast majority of task measures. This is despite the fact that all musicians were selected to have the majority of their training on the target instrument, had on average more than ten years of experience on their instrument, and were currently practicing. These results suggest that general musical experience is more important than specialized musical experience with regards to perception and production of rhythms.

  12. HTTR hydrogen production system. Structure and main specifications of mock-up test facility (Contract research)

    International Nuclear Information System (INIS)

    Kato, Michio; Aita, Hideki; Inagaki, Yoshiyuki; Hayashi, Koji; Ohashi, Hirofumi; Sato, Hiroyuki; Iwatsuki, Jin; Takada, Shoji; Inaba, Yoshitomo

    2007-03-01

    The mock-up test facility was fabricated to investigate performance of the steam generator for mitigation of the temperature fluctuation of helium gas and transient behavior of the hydrogen production system for HTTR and to obtain experimental data for verification of a dynamic analysis code. The test facility has an approximate hydrogen production capacity of 120Nm 3 /h and the steam reforming process of methane; CH 4 +H 2 O=3H 2 +CO, was used for hydrogen production of the test facility. An electric heater was used as a heat source instead of the reactor in order to heat helium gas up to 880degC (4MPa) at the chemical reactor inlet which is the same temperature as the HTTR hydrogen production system. Fabrication of the test facility was completed in February in 2002, and seven cycle operations were carried out from March in 2002 to December in 2004. This report describes the structure and main specifications of the test facility. (author)

  13. The Impact of Instrument-Specific Musical Training on Rhythm Perception and Production.

    Science.gov (United States)

    Matthews, Tomas E; Thibodeau, Joseph N L; Gunther, Brian P; Penhune, Virginia B

    2016-01-01

    Studies comparing musicians and non-musicians have shown that musical training can improve rhythmic perception and production. These findings tell us that training can result in rhythm processing advantages, but they do not tell us whether practicing a particular instrument could lead to specific effects on rhythm perception or production. The current study used a battery of four rhythm perception and production tasks that were designed to test both higher- and lower-level aspects of rhythm processing. Four groups of musicians (drummers, singers, pianists, string players) and a control group of non-musicians were tested. Within-task differences in performance showed that factors such as meter, metrical complexity, tempo, and beat phase significantly affected the ability to perceive and synchronize taps to a rhythm or beat. Musicians showed better performance on all rhythm tasks compared to non-musicians. Interestingly, our results revealed no significant differences between musician groups for the vast majority of task measures. This was despite the fact that all musicians were selected to have the majority of their training on the target instrument, had on average more than 10 years of experience on their instrument, and were currently practicing. These results suggest that general musical experience is more important than specialized musical experience with regards to perception and production of rhythms.

  14. Manual of engineering drawing technical product specification and documentation to British and international standards

    CERN Document Server

    Simmons, Colin H; Simmons, Colin

    2012-01-01

    Manual of Engineering Drawing is a comprehensive guide for experts and novices for producing engineering drawings and annotated 3D models that meet the recent BSI and ISO standards of technical product documentation and specifications. This fourth edition of the text has been updated in line with recent standard revisions and amendments. The book has been prepared for international use, and includes a comprehensive discussion of the fundamental differences between the ISO and ASME standards, as well as recent updates regarding legal components, such as copyright, patents, and other legal considerations. The text is applicable to CAD and manual drawing, and it covers the recent developments in 3D annotation and surface texture specifications. Its scope also covers the concepts of pictorial and orthographic projections, geometrical, dimensional and surface tolerancing, and the principle of duality. The text also presents numerous examples of hydraulic and electrical diagrams, applications, bearings, adhesives, ...

  15. Informing General CSCW Product Development through Cooperative Design in Specific Work Domains

    DEFF Research Database (Denmark)

    Grønbæk, Kaj; Mogensen, Preben Holst

    1997-01-01

    sharing of materials in the engineering domain. In our project, a single engineering company (Great Belt Link Ltd.) was chosen as the user organization. The paper summarizes the process from observational studies, over a future workshop and cooperative prototyping activities, to a pilot installation. We...... describe how these activities informed the general hypermedia framework and application design. Use scenarios and prototypes with example data from the users‘ daily work were used as sources both to trigger design ideas and new insights regarding work practice. Common participants in specific activities...... and general development activities supported transfer of work domain knowledge into general features of the product being developed. Mutual challenging characterized the interaction between specific cooperative analysis and design activities and general development activities. Prototypes, scenarios, materials...

  16. Obstacle detection method, obstacle removing method, device and production line for practicing the methods

    International Nuclear Information System (INIS)

    Yoneyama, Takao; Ishimatsu, Tsuneo; Komata, Hisashi; Suzuki, Keisaburo.

    1997-01-01

    The present invention provides techniques for detecting and removing obstacles, which can be applied to pipelines and vessels to be used in structures such as nuclear power structures and electric power generation facilities. Namely, when the pipelines or vessels are in any of the stages, namely, production, installation, before the use after installation and before the reuse after inspection, obstacles remaining in the pipelines and vessels are blown off by using a fluid jetting mechanism (air compressor). Elastic waves generated when the blown off obstacles abut against the pipelines and vessels are detected by using a sensor. As a result, the remaining obstacles can be detected during any one of the stages described above. The blowing is repeated till the absence of the obstacles is confirmed by elastic wave signals detected by the sensor. As a result, the remaining obstacles can be removed. (I.S.)

  17. The modality-specific organization of grammatical categories: evidence from impaired spoken and written sentence production.

    Science.gov (United States)

    Rapp, B; Caramazza, A

    1997-02-01

    We describe the case of a brain-damaged individual whose speech is characterized by difficulty with practically all words except for elements of the closed class vocabulary. In contrast, his written sentence production exhibits a complementary impairment involving the omission of closed class vocabulary items and the relative sparing of nouns. On the basis of these differences we argue: (1) that grammatical categories constitute an organizing parameter of representation and/or processing for each of the independent, modality-specific lexicons, and (2) that these observations contribute to the growing evidence that access to the orthographic and phonological forms of words can occur independently.

  18. Ruminal Methane Production on Simple Phenolic Acids Addition in in Vitro Gas Production Method

    Directory of Open Access Journals (Sweden)

    A. Jayanegara

    2009-04-01

    Full Text Available Methane production from ruminants contributes to total global methane production, which is an important contributor to global warming. In this experiment, six sources of simple phenolic acids (benzoic, cinnamic, phenylacetic, caffeic, p-coumaric and ferulic acids at two different levels (2 and 5 mM added to hay diet were evaluated for their potential to reduce enteric methane production using in vitro Hohenheim gas production method. The measured variables were gas production, methane, organic matter digestibility (OMD, and short chain fatty acids (SCFA. The results showed that addition of cinnamic, caffeic, p-coumaric and ferulic acids at 5 mM significantly (P p-coumaric > ferulic > cinnamic. The addition of simple phenols did not significantly decrease OMD. Addition of simple phenols tends to decrease total SCFA production. It was concluded that methane decrease by addition of phenolic acids was relatively small, and the effect of phenolic acids on methane decrease depended on the source and concentration applied.

  19. A method for the specific detection of resident bacteria in brine shrimp larvae.

    Science.gov (United States)

    Niu, Yufeng; Defoirdt, Tom; Rekecki, Anamaria; De Schryver, Peter; Van den Broeck, Wim; Dong, Shuanglin; Sorgeloos, Patrick; Boon, Nico; Bossier, Peter

    2012-04-01

    In this study, we describe an easy but efficient method to specifically target the intestinal resident microbiota in brine shrimp larvae during DGGE analysis, hereby excluding the interference of both transient (luminal) bacteria and body surface bacteria. This effective technique has several advantages over alternative methods, with respect of ease of use and rapidity. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. Method of preparing tritium-labelled thymidine-5'-monophosphates of high specific activity

    International Nuclear Information System (INIS)

    Filip, J.; Vesely, J.; Cihak, A.

    1976-01-01

    A method is described of preparing thymidine-5'-monophosphates labelled with tritium of high specific activity based on enzyme synthesis in vitro. Phosphorylation was carried out using the catalytic effect of an enzyme contained in the supernatant fraction prepared from Yoshida ascites carcinoma in rats. The course of the enzyme reaction can be controlled by the concentration of the individual reaction mixture components. The method described allows obtaining thymidine-5'-monophosphate of radiochemical purity better than 95%. (J.B.)

  1. Gender-specific Regulatory Challenges to Product Approval: a panel discussion.

    Science.gov (United States)

    McGregor, Alyson J; Barr, Helen; Greenberg, Marna R; Safdar, Basmah; Wildgoose, Peter; Wright, David W; Hollander, Judd E

    2014-12-01

    On May 13, 2014, a 1-hour panel discussion session titled "Gender-specific Regulatory Challenges to Product Approval" was held during the Academic Emergency Medicine consensus conference, "Gender-specific Research in Emergency Medicine: Investigate, Understand, and Translate How Gender Affects Patient Outcomes." The session sought to bring together leaders in emergency medicine (EM) research, authors, and reviewers in EM research publications, as well as faculty, fellows, residents, and students engaged in research and clinical practice. A panel was convened involving a representative from the Office of Women's Health of the U.S. Food and Drug Administration, two pharmaceutical executives, and a clinical EM researcher. The moderated discussion also involved audience members who contributed significantly to the dialogue. Historical background leading up to the session along with the main themes of the discussion are reproduced in this article. These revolve around sex- and gender-specific research, statistical analysis of sex and gender, clinical practice, financial costs associated with pharmaceutical development, adaptive design, and specific recommendations on the regulatory process as it affects the specialty of EM. © 2014 by the Society for Academic Emergency Medicine.

  2. Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

    Directory of Open Access Journals (Sweden)

    Giuseppina M. Fiorino

    2018-01-01

    Full Text Available Authenticity and traceability of food products are of primary importance at all levels of the production process, from raw materials to finished products. Authentication is also a key aspect for accurate labeling of food, which is required to help consumers in selecting appropriate types of food products. With the aim of guaranteeing the authenticity of foods, various methodological approaches have been devised over the past years, mainly based on either targeted or untargeted analyses. In this review, a brief overview of current analytical methods tailored to authenticity studies, with special regard to fishery products, is provided. Focus is placed on untargeted methods that are attracting the interest of the analytical community thanks to their rapidity and high throughput; such methods enable a fast collection of “fingerprinting signals” referred to each authentic food, subsequently stored into large database for the construction of specific information repositories. In the present case, methods capable of detecting fish adulteration/substitution and involving sensory, physicochemical, DNA-based, chromatographic, and spectroscopic measurements, combined with chemometric tools, are illustrated and commented on.

  3. Highly Specific Estrone Sulfate Antibody Production Using Hapten-Bovine Serum Albumin Conjugate And Modified Tailoring

    International Nuclear Information System (INIS)

    ELBANNA, I.M.; GAMAL, M.H.; SALEM, A.

    2009-01-01

    Estrone-3-sulfate represents an important estrogenic metabolite indicative to uterine function during early pregnancy and post-partum in animals. Exploiting preparation of less expensive estrone-3-sulfate bovine serum albumin (BSA) conjugate was persuaded for raising antiserum in rabbits. The use of estrone rabbit gamma globulin conjugate as a tollerogenic agent was used to investigate the effect on specificity of the harvested antiserum. Five male New Zealand rabbits were used. After immunization procedure, blood samples were collected and individual bleedings were evaluated for titre and specificity using estrone-3-sulfate- 3 H as a tracer. The tollerogenic pre-immunization procedure gave more specific antiserum than the conventional immunization method. Nevertheless, the titre was lower in tollerogenic than conventional method (1/3500 and 1/4900 as working final dilution, respectively). It is concluded that preparation of E1 -3-sulfate oxime-BSA gave more suitable yield with less expense as compared with previous studies. Pre-immunization injection of tollerogen gave more specific antiserum while the lower titre could be improved after further booster immunization.

  4. Using network screening methods to determine locations with specific safety issues: A design consistency case study.

    Science.gov (United States)

    Butsick, Andrew J; Wood, Jonathan S; Jovanis, Paul P

    2017-09-01

    The Highway Safety Manual provides multiple methods that can be used to identify sites with promise (SWiPs) for safety improvement. However, most of these methods cannot be used to identify sites with specific problems. Furthermore, given that infrastructure funding is often specified for use related to specific problems/programs, a method for identifying SWiPs related to those programs would be very useful. This research establishes a method for Identifying SWiPs with specific issues. This is accomplished using two safety performance functions (SPFs). This method is applied to identifying SWiPs with geometric design consistency issues. Mixed effects negative binomial regression was used to develop two SPFs using 5 years of crash data and over 8754km of two-lane rural roadway. The first SPF contained typical roadway elements while the second contained additional geometric design consistency parameters. After empirical Bayes adjustments, sites with promise (SWiPs) were identified. The disparity between SWiPs identified by the two SPFs was evident; 40 unique sites were identified by each model out of the top 220 segments. By comparing sites across the two models, candidate road segments can be identified where a lack design consistency may be contributing to an increase in expected crashes. Practitioners can use this method to more effectively identify roadway segments suffering from reduced safety performance due to geometric design inconsistency, with detailed engineering studies of identified sites required to confirm the initial assessment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  5. Method of treating final products from flue gas desulfurization

    International Nuclear Information System (INIS)

    Bloss, W.; Mohn, U.

    1984-01-01

    A method of treating final products from a flue gas desulfurization. The flue gas desulfurization is carried out by the absorption of sulfur oxide in a spray dryer with a suspension which contains lime, or in a reactor with a dry, fine-grained, absorbent which contains lime. Prior to desulfurization, the fly ash carried along by the flue gas which is to be desulfurized is separated entirely, partially, or not at all from the flue gas, and the final products from the flue gas desulfurization, prior to any further treatment thereof, amount to 1-99% by weight, preferably 1-70% by weight, of fly ash, and 1-99% by weight, preferably 30-99% by weight, of the sum of the desulfurization products, preferably calcium sulfite hemihydrate, and/or calcium sulfite, and/or calcium sulfate dyhydrate, and/or calcium sulfate hemihydrate, and/or calcium sulfate, as well as residue of the absorbent. The reduction of the amount of calcium sulfite is implemented by a dry oxidation with air

  6. INNOVATIVE USE OF QUALITY MANAGEMENT METHODS FOR PRODUCT IMPROVEMENT

    Directory of Open Access Journals (Sweden)

    Katarzyna MIDOR

    2016-10-01

    Full Text Available Organisations constantly look for new, innovative solutions and methods which could be used to improve their efficiency and increase the quality of their products. Identifying the causes for returns is an important issue for modern compa-nies, as returns are the cause for the increase in production costs and, most importantly, the loss of credibility in the eyes of the client. Therefore, for the company to be able to sustain or strengthen its position on the market, it has to follow the rules of quality management. Especially important is the rule of constant improvement. This rule is primarily connected with preventing errors and defects from occurring at all the stages of the production process. To achieve that, one must, among other things, use quality management tools. The article presents an analysis of causes for returns of a vibrating screen produced by a company which manufactures machinery and equipment for the extractive industry, using quality management tools such as the Ishikawa diagram and Pareto analysis. The analysis allowed for the identifi-cation of the causes of client returns which could not be previously identified, and proposing solutions for them.

  7. Innovative Use of Quality Management Methods for Product Improvement

    Science.gov (United States)

    Midor, Katarzyna; Žarnovský, Jozef

    2016-12-01

    Organisations constantly look for new, innovative solutions and methods which could be used to improve their efficiency and increase the quality of their products. Identifying the causes for returns is an important issue for modern companies, as returns are the cause for the increase in production costs and, most importantly, the loss of credibility in the eyes of the client. Therefore, for the company to be able to sustain or strengthen its position on the market, it has to follow the rules of quality management. Especially important is the rule of constant improvement. This rule is primarily connected with preventing errors and defects from occurring at all the stages of the production process. To achieve that, one must, among other things, use quality management tools. The article presents an analysis of causes for returns of a vibrating screen produced by a company which manufactures machinery and equipment for the extractive industry, using quality management tools such as the Ishikawa diagram and Pareto analysis. The analysis allowed for the identification of the causes of client returns which could not be previously identified, and proposing solutions for them.

  8. A Wavelet-Based Optimization Method for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Maurizio Carlini

    2018-02-01

    Full Text Available On a global scale many countries are still heavily dependent on crude oil to produce energy and fuel for transport, with a resulting increase of atmospheric pollution. A possible solution to obviate this problem is to find eco-sustainable energy sources. A potential choice could be the use of biodiesel as fuel. The work presented aims to characterise the transesterification reaction of waste peanut frying oil using colour analysis and wavelet analysis. The biodiesel production, with the complete absence of mucilages, was evaluated through a suitable set of energy wavelet coefficients and scalograms. The physical characteristics of the biodiesel are influenced by mucilages. In particular the viscosity, that is a fundamental parameter for the correct use of the biodiesel, might be compromised. The presence of contaminants in the samples can often be missed by visual analysis. The low and high frequency wavelet analysis, by investigating the energy change of wavelet coefficient, provided a valid characterisation of the quality of the samples, related to the absence of mucilages, which is consistent with the experimental results. The proposed method of this work represents a preliminary analysis, before the subsequent chemical physical analysis, that can be develop during the production phases of the biodiesel in order to optimise the process, avoiding the presence of impurities in suspension in the final product.

  9. Risk-based analysis methods applied to nuclear power plant technical specifications

    International Nuclear Information System (INIS)

    Wagner, D.P.; Minton, L.A.; Gaertner, J.P.

    1989-01-01

    A computer-aided methodology and practical applications of risk-based evaluation of technical specifications are described. The methodology, developed for use by the utility industry, is a part of the overall process of improving nuclear power plant technical specifications. The SOCRATES computer program uses the results of a probabilistic risk assessment or a system-level risk analysis to calculate changes in risk due to changes in the surveillance test interval and/or the allowed outage time stated in the technical specification. The computer program can accommodate various testing strategies (such as staggered or simultaneous testing) to allow modeling of component testing as it is carried out at the plant. The methods and computer program are an integral part of a larger decision process aimed at determining benefits from technical specification changes. These benefits can include cost savings to the utilities by reducing forced shutdowns and decreasing labor requirements for test and maintenance activities, with no adverse impacts on risk. The methodology and the SOCRATES computer program have been used extensively toe valuate several actual technical specifications in case studies demonstrating the methods. Summaries of these applications demonstrate the types of results achieved and the usefulness of the risk-based evaluation in improving the technical specifications

  10. A method for projecting age-specific mortality rates for certain causes of death

    International Nuclear Information System (INIS)

    Leggett, R.W.; Crawford, D.J.

    1981-01-01

    A method is presented for projecting mortality rates for certain causes on the basis of observed rates during past years. This method arose from a study of trends in age-specific mortality rates for respiratory cancers, and for heuristic purposes it is shown how the method can be developed from certain theories of cancer induction. However, the method is applicable in the more common situation in which the underlying physical processes cannot be modeled with any confidence but the mortality rates are approximable over short time intervals by functions of the form a exp(bt), where b may vary in a continuous, predictable fashion as the time interval is varied. It appears from applications to historical data that this projection method is in some cases a substantial improvement over conventional curve-fitting methods and often uncovers trends which are not from observed data

  11. Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoense with broad substrate specificity

    Science.gov (United States)

    2013-01-01

    Background Efficient conversion of lignocellulosic biomass to optically pure lactic acid is a key challenge for the economical production of biodegradable poly-lactic acid. A recently isolated strain, Thermoanaerobacterium aotearoense SCUT27, is promising as an efficient lactic acid production bacterium from biomass due to its broad substrate specificity. Additionally, its strictly anaerobic and thermophilic characteristics suppress contamination from other microoragnisms. Herein, we report the significant improvements of concentration and yield in lactic acid production from various lignocellulosic derived sugars, achieved by the carbon flux redirection through homologous recombination in T. aotearoense SCUT27. Results T. aotearoense SCUT27 was engineered to block the acetic acid formation pathway to improve the lactic acid production. The genetic manipulation resulted in 1.8 and 2.1 fold increase of the lactic acid yield using 10 g/L of glucose or 10 g/L of xylose as substrate, respectively. The maximum l-lactic acid yield of 0.93 g/g glucose with an optical purity of 99.3% was obtained by the engineered strain, designated as LA1002, from 50 g/L of substrate, which is very close to the theoretical value (1.0 g/g of glucose). In particular, LA1002 produced lactic acid at an unprecedented concentration up to 3.20 g/L using 10 g/L xylan as the single substrate without any pretreatment after 48 h fermentation. The non-sterilized fermentative production of l-lactic acid was also carried out, achieving values of 44.89 g/L and 0.89 g/g mixed sugar for lactic acid concentration and yield, respectively. Conclusions Blocking acetic acid formation pathway in T. aotearoense SCUT27 increased l-lactic acid production and yield dramatically. To our best knowledge, this is the best performance of fermentation on lactic acid production using xylan as the sole carbon source, considering the final concentration, yield and fermentation time. In addition, it should be

  12. Radiation method for hygienization food and agriculture products

    International Nuclear Information System (INIS)

    Migdal, W.

    1995-01-01

    The irradiation of food and agriculture products have been used for several goals. That are: disinsectization, disinfection, sprout inhibition and retardation of mattering. In the frames of the national programme on the application of irradiation for food preservation and hygienization an experimental plant for electron beam processing has been established in INCT (Institute of Nuclear Chemistry and Technology). The pilot plant is equipped with a small research accelerator Pilot (10 MeV, 1 kW) and and industrial unit Elektronika (10 MeV, 10 kW). This allows both laboratory and full technological scale testing of the elaborated process to be conducted. The industrial unit is being equipped with e-/X conversion target, for high density products irradiation. On the basis of the research there were performed at different scientific institutions in Poland, health authorities have issued permissions for permanent treatment of spices, garlic, onions and temporary permissions for mushrooms, and potatoes. Dosimetric methods have been elaborated for the routine use at the plant. In the INCT laboratory methods for the control of e-/X treated food have been established. (author)

  13. Biodiesel Production from Microalgae by Extraction – Transesterification Method

    Directory of Open Access Journals (Sweden)

    Nguyen Thi Phuong Thao

    2013-11-01

    Full Text Available The environmental impact of using petroleum fuels has led to a quest to find a suitable alternative fuel source. In this study, microalgae were explored as a highly potential feedstock to produce biodiesel fuel. Firstly, algal oil is extracted from algal biomass by using organic solvents (n–hexan.  Lipid is contained in microalgae up to 60% of their weight. Then, Biodiesel is created through a chemical reaction known as transesterification between algal oil and alcohol (methanol with strong acid (such as H2SO4 as the catalyst. The extraction – transesterification method resulted in a high biodiesel yield (10 % of algal biomass and high FAMEs content (5.2 % of algal biomass. Biodiesel production from microalgae was studied through experimental investigation of transesterification conditions such as reaction time, methanol to oil ration and catalyst dosage which are deemed to have main impact on reaction conversion efficiency. All the parameters which were characterized for purified biodiesel such as free glycerin, total glycerin, flash point, sulfur content were analyzed according to ASTM standardDoi: http://dx.doi.org/10.12777/wastech.1.1.6-9Citation:  Thao, N.T.P., Tin, N.T., and Thanh, B.X. 2013. Biodiesel Production from Microalgae by Extraction – Transesterification Method. Waste Technology 1(1:6-9. Doi: http://dx.doi.org/10.12777/wastech.1.1.6-9

  14. Adaptive designs based on the truncated product method

    Directory of Open Access Journals (Sweden)

    Neuhäuser Markus

    2005-09-01

    Full Text Available Abstract Background Adaptive designs are becoming increasingly important in clinical research. One approach subdivides the study into several (two or more stages and combines the p-values of the different stages using Fisher's combination test. Methods Alternatively to Fisher's test, the recently proposed truncated product method (TPM can be applied to combine the p-values. The TPM uses the product of only those p-values that do not exceed some fixed cut-off value. Here, these two competing analyses are compared. Results When an early termination due to insufficient effects is not appropriate, such as in dose-response analyses, the probability to stop the trial early with the rejection of the null hypothesis is increased when the TPM is applied. Therefore, the expected total sample size is decreased. This decrease in the sample size is not connected with a loss in power. The TPM turns out to be less advantageous, when an early termination of the study due to insufficient effects is possible. This is due to a decrease of the probability to stop the trial early. Conclusion It is recommended to apply the TPM rather than Fisher's combination test whenever an early termination due to insufficient effects is not suitable within the adaptive design.

  15. Defining the Undefinable: Operationalization of Methods to Identify Specific Learning Disabilities among Practicing School Psychologists

    Science.gov (United States)

    Cottrell, Joseph M.; Barrett, Courtenay A.

    2016-01-01

    Accurate and consistent identification of students with specific learning disabilities (SLDs) is crucial; however, state and district guidelines regarding identification methods lack operationalization and are inconsistent throughout the United States. In the current study, the authors surveyed 471 school psychologists about "school" SLD…

  16. WMAXC: a weighted maximum clique method for identifying condition-specific sub-network.

    Directory of Open Access Journals (Sweden)

    Bayarbaatar Amgalan

    Full Text Available Sub-networks can expose complex patterns in an entire bio-molecular network by extracting interactions that depend on temporal or condition-specific contexts. When genes interact with each other during cellular processes, they may form differential co-expression patterns with other genes across different cell states. The identification of condition-specific sub-networks is of great importance in investigating how a living cell adapts to environmental changes. In this work, we propose the weighted MAXimum clique (WMAXC method to identify a condition-specific sub-network. WMAXC first proposes scoring functions that jointly measure condition-specific changes to both individual genes and gene-gene co-expressions. It then employs a weaker formula of a general maximum clique problem and relates the maximum scored clique of a weighted graph to the optimization of a quadratic objective function under sparsity constraints. We combine a continuous genetic algorithm and a projection procedure to obtain a single optimal sub-network that maximizes the objective function (scoring function over the standard simplex (sparsity constraints. We applied the WMAXC method to both simulated data and real data sets of ovarian and prostate cancer. Compared with previous methods, WMAXC selected a large fraction of cancer-related genes, which were enriched in cancer-related pathways. The results demonstrated that our method efficiently captured a subset of genes relevant under the investigated condition.

  17. To Demonstrate the Specificity of an Enzymatic Method for Plasma Paracetamol Estimation.

    Science.gov (United States)

    O'Mullane, John A.

    1987-01-01

    Describes an experiment designed to introduce biochemistry students to the specificity of an analytical method which uses an enzyme to quantitate its substrate. Includes the use of toxicity charts together with the concept of the biological half-life of a drug. (TW)

  18. Disregarding population specificity: its influence on the sex assessment methods from the tibia

    Czech Academy of Sciences Publication Activity Database

    Kotěrová, A.; Velemínská, J.; Dupej, J.; Brzobohatá, Hana; Pilný, A.; Brůžek, J.

    2017-01-01

    Roč. 131, č. 1 (2017), s. 251-261 ISSN 0937-9827 Institutional support: RVO:67985912 Keywords : forensic anthropology population data * sex determination * tibia * population specificity * discriminant function analysis * GAME method Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Archaeology Impact factor: 2.382, year: 2016

  19. Application of specific gravity method for normalization of urinary excretion rates of radionuclides

    International Nuclear Information System (INIS)

    Thakur, Smita S.; Yadav, J.R.; Rao, D.D.

    2015-01-01

    In vitro bioassay monitoring is based on the determination of activity concentration in biological samples excreted from the body and is most suitable for alpha and beta emitters. For occupational workers handling actinides in reprocessing facilities possibility of internal exposure exists and urine assay is preferred method for monitoring such exposure. Urine samples collected for 24 h duration, is the true representative of bioassay sample and hence in the case of insufficient collection time, specific gravity applied method of normalization of urine sample is used. The present study reports the data of specific gravity generated for controlled group of Indian population by the use of densitometer and its application in urinary sample activity normalization. The average specific gravity value obtained for the controlled group was 1.008±0.005 gm/ml. (author)

  20. Phonon spectrum of YBCO obtained by specific heat inversion method for real data

    CERN Document Server

    Tao Wen; Dai Xian Xi; Dai Ji Xin; Evenson, W E

    2003-01-01

    In this paper, the phonon spectrum of YBCO is obtained from experimental specific heat data by an exact inversion formula with a parameter for eliminating divergences. The results can be compared to those of neutron inelastic scattering, which can only be carried out in a few laboratories. Some key points of specific heat-phonon spectrum inversion (SPI) theory and a method of asymptotic behaviour control are discussed. An improved unique existence theorem is presented, and a universal function set for numerical calculation of SPI is calculated with high accuracy, which makes the inversion method applicable and convenient in practice. This is the first time specific heat-phonon SPI has been realized for a concrete system.

  1. Granulated Silica Method for the Fiber Preform Production

    Directory of Open Access Journals (Sweden)

    Sönke Pilz

    2017-07-01

    Full Text Available During the past few years, we have studied the granulated silica method as a versatile and cost effective way of fiber preform production and the sol-gel method. Until now, we have used the sol-gel technology together with an iterative re-melting and milling step in order to produce rare earth or transition metal doped granular material for the granulated silica method. Here, we present that the iterative re-melting (laser-assisted and milling step is no longer needed to reach a high homogeneity. The sol-gel method also offers a high degree of compositional flexibility with respect to dopants; it further facilitates achieving high concentrations, even in cases when several dopants are used. We employed optical active doped sol-gel derived granulate for the fiber core, whereas pure or index-raised granulated silica has been employed for the cladding. Based on the powder-in-tube technique, where silica glass tubes are appropriately filled with these granular materials, fibers has been directly drawn (“fiber rapid prototyping”, or eventually after an additional optional quality enhancing vitrification step. The powder-in-tube technique is also ideally suited for the preparation of microstructured optical fibers.

  2. Detection methods for mycotoxins in cereal grains and cereal products

    Directory of Open Access Journals (Sweden)

    Pascale Michelangelo N.

    2009-01-01

    Full Text Available Analytical methods for mycotoxins in cereals and cereal-based products require three major steps, including extraction, clean-up (to eliminate interferences from the extract and concentrate the analyte, and detection/determination of the toxin (by using suitable analytical instruments/technologies. Clean-up is essential for the analysis of mycotoxins at trace levels, and involves the use of solid phase extraction and multifunctional (e.g. MycoSep® or immunoaffinity columns. Different chromatographic methods are commonly used for quantitative determination of mycotoxins, including gas-chromatography (GC coupled with electron capture, flame ionization or mass spectrometry (MS detectors (mainly for type-A trichothecenes, and high-performance liquid chromatography (HPLC coupled with ultraviolet, diode array, fluorescence or MS detectors. The choice of method depends on the matrix and the mycotoxin to be analyzed. Liquid chromatography-tandem mass spectrometry (LC-MS/MS is spreading rapidly as a promising technique for simultaneous screening, identification and quantitative determination of a large number of mycotoxins. In addition, commercial immunometric assays, such as enzyme-linked immunosorbent assays (ELISA, are frequently used for screening purposes as well. Recently, a variety of emerging methods have been proposed for the analysis of mycotoxins in cereals based on novel technologies, including immunochromatography (i.e. lateral flow devices, fluorescence polarization immunoassays (FPIA, infrared spectroscopy (FT-NIR, molecularly imprinted polymers (MIPs and optical biosensors.

  3. Genes related to xylose fermentation and methods of using same for enhanced biofuel production

    Science.gov (United States)

    Wohlbach, Dana J.; Gasch, Audrey P.

    2014-08-05

    The present invention provides isolated gene sequences involved in xylose fermentation and related recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast useful for biofuel production are also provided.

  4. Method for Hot Real-Time Sampling of Gasification Products

    Energy Technology Data Exchange (ETDEWEB)

    Pomeroy, Marc D [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-09-29

    The Thermochemical Process Development Unit (TCPDU) at the National Renewable Energy Laboratory (NREL) is a highly instrumented half-ton/day pilot scale plant capable of demonstrating industrially relevant thermochemical technologies from lignocellulosic biomass conversion, including gasification. Gasification creates primarily Syngas (a mixture of Hydrogen and Carbon Monoxide) that can be utilized with synthesis catalysts to form transportation fuels and other valuable chemicals. Biomass derived gasification products are a very complex mixture of chemical components that typically contain Sulfur and Nitrogen species that can act as catalysis poisons for tar reforming and synthesis catalysts. Real-time hot online sampling techniques, such as Molecular Beam Mass Spectrometry (MBMS), and Gas Chromatographs with Sulfur and Nitrogen specific detectors can provide real-time analysis providing operational indicators for performance. Sampling typically requires coated sampling lines to minimize trace sulfur interactions with steel surfaces. Other materials used inline have also shown conversion of sulfur species into new components and must be minimized. Sample line Residence time within the sampling lines must also be kept to a minimum to reduce further reaction chemistries. Solids from ash and char contribute to plugging and must be filtered at temperature. Experience at NREL has shown several key factors to consider when designing and installing an analytical sampling system for biomass gasification products. They include minimizing sampling distance, effective filtering as close to source as possible, proper line sizing, proper line materials or coatings, even heating of all components, minimizing pressure drops, and additional filtering or traps after pressure drops.

  5. Methods for analysis of bacterial autoinducer-2 production.

    Science.gov (United States)

    Taga, Michiko E

    2005-07-01

    The quorum-sensing signal molecule autoinducer-2 (AI-2) is produced by over 50 diverse bacterial species and controls many different processes, including antibiotic production, biofilm formation, and virulence. AI-2 production often varies according to growth phase, media conditions, and the presence of specific factors. This unit describes a biological assay for AI-2 activity produced by a bacterial strain of interest. The assay employs an AI-2 reporter strain, Vibrio harveyi BB170, which produces light in response to AI-2. In the first stage of the assay, culture fluids of the bacterial strain of interest are collected over a time course of growth and filtered to remove cells. In the next stage, these culture fluids are mixed with BB170, and the light produced in response to AI-2 in the culture fluids is measured using a luminometer. BB170 is exquisitely sensitive to AI-2, and therefore, even low amounts of AI-2 can be detected using this bioassay.

  6. Case-specific comparison of water pollution control alternatives in peat production

    International Nuclear Information System (INIS)

    Savolainen, M.; Kaasinen, A.; Heikkinen, K.; Ihme, R.; Kaemae, T.; Alasaarela, E.

    1996-01-01

    The present practice water pollution control in peat production and the elements of planning were analyzed, the water purification methods were classified and their weaknesses estimated. Furthermore, the cost of the water purification constructions was estimated and their significance for the watercourses evaluated. 54 peat production plans were chosen from the catchment areas of the rivers Iijoki, Siikajoki and Pyhaejoki. The suitability of the chosen water pollution control methods was evaluated on the basis of the plans and, further, on the basis of field surveys. The suitability of the purification methods to practical water pollution control was assessed by making plans for 15 peat mining areas. There is a need to develop the planning and implementation of water pollution control in peat mining. The methods that are used do not always work in the expected way in practice. Despite this planning is compatible with the water protection program and the regulations that are in force. The study gives a good idea of how to update the planning instructions for water pollution control. The accompanying report includes plan for 11 peat mining areas. (orig.)

  7. Case-specific comparison of water pollution control alternatives in peat production; Turvetuotannon vesiensuojeluvaihtoehtojen tapauskohtainen vertailu

    Energy Technology Data Exchange (ETDEWEB)

    Savolainen, M.; Kaasinen, A.; Heikkinen, K.; Ihme, R.; Kaemae, T.; Alasaarela, E.

    1996-12-31

    The present practice water pollution control in peat production and the elements of planning were analyzed, the water purification methods were classified and their weaknesses estimated. Furthermore, the cost of the water purification constructions was estimated and their significance for the watercourses evaluated. 54 peat production plans were chosen from the catchment areas of the rivers Iijoki, Siikajoki and Pyhaejoki. The suitability of the chosen water pollution control methods was evaluated on the basis of the plans and, further, on the basis of field surveys. The suitability of the purification methods to practical water pollution control was assessed by making plans for 15 peat mining areas. There is a need to develop the planning and implementation of water pollution control in peat mining. The methods that are used do not always work in the expected way in practice. Despite this planning is compatible with the water protection program and the regulations that are in force. The study gives a good idea of how to update the planning instructions for water pollution control. The accompanying report includes plan for 11 peat mining areas. (orig.)

  8. A comparison study of size-specific dose estimate calculation methods

    Energy Technology Data Exchange (ETDEWEB)

    Parikh, Roshni A. [Rainbow Babies and Children' s Hospital, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Department of Radiology, Cleveland, OH (United States); University of Michigan Health System, Department of Radiology, Ann Arbor, MI (United States); Wien, Michael A.; Jordan, David W.; Ciancibello, Leslie; Berlin, Sheila C. [Rainbow Babies and Children' s Hospital, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Department of Radiology, Cleveland, OH (United States); Novak, Ronald D. [Rainbow Babies and Children' s Hospital, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Department of Radiology, Cleveland, OH (United States); Rebecca D. Considine Research Institute, Children' s Hospital Medical Center of Akron, Center for Mitochondrial Medicine Research, Akron, OH (United States); Klahr, Paul [CT Clinical Science, Philips Healthcare, Highland Heights, OH (United States); Soriano, Stephanie [Rainbow Babies and Children' s Hospital, University Hospitals Cleveland Medical Center, Case Western Reserve University School of Medicine, Department of Radiology, Cleveland, OH (United States); University of Washington, Department of Radiology, Seattle, WA (United States)

    2018-01-15

    The size-specific dose estimate (SSDE) has emerged as an improved metric for use by medical physicists and radiologists for estimating individual patient dose. Several methods of calculating SSDE have been described, ranging from patient thickness or attenuation-based (automated and manual) measurements to weight-based techniques. To compare the accuracy of thickness vs. weight measurement of body size to allow for the calculation of the size-specific dose estimate (SSDE) in pediatric body CT. We retrospectively identified 109 pediatric body CT examinations for SSDE calculation. We examined two automated methods measuring a series of level-specific diameters of the patient's body: method A used the effective diameter and method B used the water-equivalent diameter. Two manual methods measured patient diameter at two predetermined levels: the superior endplate of L2, where body width is typically most thin, and the superior femoral head or iliac crest (for scans that did not include the pelvis), where body width is typically most thick; method C averaged lateral measurements at these two levels from the CT projection scan, and method D averaged lateral and anteroposterior measurements at the same two levels from the axial CT images. Finally, we used body weight to characterize patient size, method E, and compared this with the various other measurement methods. Methods were compared across the entire population as well as by subgroup based on body width. Concordance correlation (ρ{sub c}) between each of the SSDE calculation methods (methods A-E) was greater than 0.92 across the entire population, although the range was wider when analyzed by subgroup (0.42-0.99). When we compared each SSDE measurement method with CTDI{sub vol,} there was poor correlation, ρ{sub c}<0.77, with percentage differences between 20.8% and 51.0%. Automated computer algorithms are accurate and efficient in the calculation of SSDE. Manual methods based on patient thickness provide

  9. IP cores design from specifications to production modeling, verification, optimization, and protection

    CERN Document Server

    Mohamed, Khaled Salah

    2016-01-01

    This book describes the life cycle process of IP cores, from specification to production, including IP modeling, verification, optimization, and protection. Various trade-offs in the design process are discussed, including  those associated with many of the most common memory cores, controller IPs  and system-on-chip (SoC) buses. Readers will also benefit from the author’s practical coverage of new verification methodologies. such as bug localization, UVM, and scan-chain.  A SoC case study is presented to compare traditional verification with the new verification methodologies. ·         Discusses the entire life cycle process of IP cores, from specification to production, including IP modeling, verification, optimization, and protection; ·         Introduce a deep introduction for Verilog for both implementation and verification point of view.  ·         Demonstrates how to use IP in applications such as memory controllers and SoC buses. ·         Describes a new ver...

  10. Bordetella bronchiseptica antigen enhances the production of Mycoplasma hyopneumoniae antigen-specific immunoglobulin G in mice.

    Science.gov (United States)

    Yim, Seol-Hwa; Hahn, Tae-Wook; Joo, Hong-Gu

    2017-09-30

    We previously demonstrated that Bordetella ( B .) bronchiseptica antigen (Ag) showed high immunostimulatory effects on mouse bone marrow cells (BMs) while Mycoplasma ( M .) hyopneumoniae Ag showed low effects. The focus of this study was to determine if B. bronchiseptica Ag can enhance the M. hyopneumoniae Ag-specific immune response and whether the host's immune system can recognize both Ags. MTT assay results revealed that each or both Ags did not significantly change BM metabolic activity. Flow cytometry analysis using carboxyfluorescein succinimidyl ester showed that B. bronchiseptica Ag can promote the division of BMs. In cytokine and nitric oxide (NO) assays, B. bronchiseptica Ag boosted production of tumor necrosis factor-alpha in M. hyopneumoniae Ag-treated BMs, and combined treatment with both Ags elevated the level of NO in BMs compared to that from treatment of M. hyopneumoniae Ag alone. Immunoglobulin (Ig)G enzyme-linked immunosorbent assay using the sera of Ag-injected mice clearly indicated that B. bronchiseptica Ag can increase the production of M. hyopneumoniae Ag-specific IgG. This study provided information valuable in the development of M. hyopneumoniae vaccines and showed that B. bronchiseptica Ag can be used both as a vaccine adjuvant and as a vaccine Ag.

  11. IMPLEMENTING THE 5S METHOD IN THE PRODUCTION SYSTEM OF DACIA & RENAULT

    Directory of Open Access Journals (Sweden)

    Marin CIOBANU

    2014-11-01

    Full Text Available The aim of this paper is to demonstrate the usefulness of the 5S method in addressing the issues of continuous improvement of productivity and quality of work. There are presented the 5 steps, techniques and the adjacent tools. Solutions implemented in the production system Dacia & Renault are indicated. This article provides a practical framework. The 5S method follows the Deming PDCA cycle which constitutes the starting point of the SDCA cycle of continuous improvement of the company's activities in order to obtain excellence. The implementing time of the method depends on the resources and materials allocated. Deployment of 5S is achieved by a scheme of "3+2": the first 3S are tangible and immediate actions, the last 2S appeal to specific management actions. The efficacy of their own workplace in total autonomy creates comfort in the work environment and gives personal benefits to workers.

  12. Handbook of methods for risk-based analysis of technical specifications

    International Nuclear Information System (INIS)

    Samanta, P.K.; Kim, I.S.; Mankamo, T.; Vesely, W.E.

    1996-01-01

    Technical Specifications (TS) requirements for nuclear power plants define the Limiting Conditions for Operations (LCOs) and Surveillance Requirements (SRs) to assure safety during operation. In general, these requirements are based on deterministic analyses and engineering judgments. Improvements in these requirements are facilitated by the availability of plant-specific Probabilistic Risk Assessments (PRAs). The US Nuclear Regulatory Commission (USNRC) Office of Research sponsored research to develop systematic, risk-based methods to improve various aspects of TS requirements. A handbook of methods summarizing such risk-based approaches has been completed in 1994. It is expected that this handbook will provide valuable input to NRC's present work in developing guidance for using PRA in risk-informed regulation. The handbook addresses reliability and risk-based methods for evaluating allowed outage times (AOTs), action statements requiring shutdown where shutdown risk may be substantial, surveillance test intervals (STIs), managing plant configurations, and scheduling maintenance

  13. Handbook of methods for risk-based analysis of technical specification requirements

    International Nuclear Information System (INIS)

    Samanta, P.K.; Vesely, W.E.

    1994-01-01

    Technical Specifications (TS) requirements for nuclear power plants define the Limiting Conditions for Operation (LCOs) and Surveillance Requirements (SRs) to assure safety during operation. In general, these requirements were based on deterministic analysis and engineering judgments. Experiences with plant operation indicate that some elements of the requirements are unnecessarily restrictive, while others may not be conducive to safety. Improvements in these requirements are facilitated by the availability of plant specific Probabilistic Safety Assessments (PSAs). The use of risk and reliability-based methods to improve TS requirements has gained wide interest because these methods can: Quantitatively evaluate the risk and justify changes based on objective risk arguments; Provide a defensible basis for these requirements for regulatory applications. The US NRC Office of Research is sponsoring research to develop systematic risk-based methods to improve various aspects of TS requirements. The handbook of methods, which is being prepared, summarizes such risk-based methods. The scope of the handbook includes reliability and risk-based methods for evaluating allowed outage times (AOTs), action statements requiring shutdown where shutdown risk may be substantial, surveillance test intervals (STIs), defenses against common-cause failures, managing plant configurations, and scheduling maintenances. For each topic, the handbook summarizes methods of analysis and data needs, outlines the insights to be gained, lists additional references, and presents examples of evaluations

  14. Handbook of methods for risk-based analysis of Technical Specification requirements

    International Nuclear Information System (INIS)

    Samanta, P.K.; Vesely, W.E.

    1993-01-01

    Technical Specifications (TS) requirements for nuclear power plants define the Limiting Conditions for Operation (LCOs) and Surveillance Requirements (SRs) to assure safety during operation. In general, these requirements were based on deterministic analysis and engineering judgments. Experiences with plant operation indicate that some elements of the requirements are unnecessarily restrictive, while others may not be conducive to safety. Improvements in these requirements are facilitated by the availability of plant specific Probabilistic Safety Assessments (PSAs). The use of risk and reliability-based methods to improve TS requirements has gained wide interest because these methods can: quantitatively evaluate the risk impact and justify changes based on objective risk arguments. Provide a defensible basis for these requirements for regulatory applications. The United States Nuclear Regulatory Commission (USNRC) Office of Research is sponsoring research to develop systematic risk-based methods to improve various aspects of TS requirements. The handbook of methods, which is being prepared, summarizes such risk-based methods. The scope of the handbook includes reliability and risk-based methods for evaluating allowed outage times (AOTs), action statements requiring shutdown where shutdown risk may be substantial, surveillance test intervals (STIs), defenses against common-cause failures, managing plant configurations, and scheduling maintenances. For each topic, the handbook summarizes methods of analysis and data needs, outlines the insights to be gained, lists additional references, and presents examples of evaluations

  15. A glutamate/aspartate switch controls product specificity in a protein arginine methyltransferase

    Energy Technology Data Exchange (ETDEWEB)

    Debler, Erik W.; Jain, Kanishk; Warmack, Rebeccah A.; Feng, You; Clarke, Steven G.; Blobel, Günter; Stavropoulos, Pete

    2016-02-08

    Trypanosoma brucei PRMT7 (TbPRMT7) is a protein arginine methyltransferase (PRMT) that strictly monomethylates various substrates, thus classifying it as a type III PRMT. However, the molecular basis of its unique product specificity has remained elusive. Here, we present the structure of TbPRMT7 in complex with its cofactor product S-adenosyl-L-homocysteine (AdoHcy) at 2.8 Å resolution and identify a glutamate residue critical for its monomethylation behavior. TbPRMT7 comprises the conserved methyltransferase and β-barrel domains, an N-terminal extension, and a dimerization arm. The active site at the interface of the N-terminal extension, methyltransferase, and β-barrel domains is stabilized by the dimerization arm of the neighboring protomer, providing a structural basis for dimerization as a prerequisite for catalytic activity. Mutagenesis of active-site residues highlights the importance of Glu181, the second of the two invariant glutamate residues of the double E loop that coordinate the target arginine in substrate peptides/proteins and that increase its nucleophilicity. Strikingly, mutation of Glu181 to aspartate converts TbPRMT7 into a type I PRMT, producing asymmetric dimethylarginine (ADMA). Isothermal titration calorimetry (ITC) using a histone H4 peptide showed that the Glu181Asp mutant has markedly increased affinity for monomethylated peptide with respect to the WT, suggesting that the enlarged active site can favorably accommodate monomethylated peptide and provide sufficient space for ADMA formation. In conclusion, these findings yield valuable insights into the product specificity and the catalytic mechanism of protein arginine methyltransferases and have important implications for the rational (re)design of PRMTs.

  16. Cause-specific mortality in Finnish ferrochromium and stainless steel production workers.

    Science.gov (United States)

    Huvinen, M; Pukkala, E

    2016-04-01

    Although stainless steel has been produced for more than a hundred years, exposure-related mortality data for production workers are limited. To describe cause-specific mortality in Finnish ferrochromium and stainless steel workers. We studied Finnish stainless steel production chain workers employed between 1967 and 2004, from chromite mining to cold rolling of stainless steel, divided into sub-cohorts by production units with specific exposure patterns. We obtained causes of death for the years 1971-2012 from Statistics Finland. We calculated standardized mortality ratios (SMRs) as ratios of observed and expected numbers of deaths based on population mortality rates of the same region. Among 8088 workers studied, overall mortality was significantly decreased (SMR 0.77; 95% confidence interval [CI] 0.70-0.84), largely due to low mortality from diseases of the circulatory system (SMR 0.71; 95% CI 0.61-0.81). In chromite mine, stainless steel melting shop and metallurgical laboratory workers, the SMR for circulatory disease was below 0.4 (SMR 0.33; 95% CI 0.07-0.95, SMR 0.22; 95% CI 0.05-0.65 and SMR 0.16; 95% CI 0.00-0.90, respectively). Mortality from accidents (SMR 0.84; 95% CI 0.67-1.04) and suicides (SMR 0.72; 95% CI 0.56-0.91) was also lower than in the reference population. Working in the Finnish ferrochromium and stainless steel industry appears not to be associated with increased mortality. © The Author 2015. Published by Oxford University Press on behalf of the Society of Occupational Medicine.

  17. Chapter 1: Introduction. The Uniform Methods Project: Methods for Determining Energy-Efficiency Savings for Specific Measures

    Energy Technology Data Exchange (ETDEWEB)

    Li, Michael [Dept. of Energy (DOE), Washington DC (United States). Office of Energy Efficiency and Renewable Energy; Haeri, Hossein [The Cadmus Group, Portland, OR (United States); Reynolds, Arlis [The Cadmus Group, Portland, OR (United States)

    2017-09-28

    This chapter provides a set of model protocols for determining energy and demand savings that result from specific energy efficiency measures implemented through state and utility efficiency programs. The methods described here are approaches that are or are among the most commonly used and accepted in the energy efficiency industry for certain measures or programs. As such, they draw from the existing body of research and best practices for energy efficiency program evaluation, measurement, and verification (EM&V). These protocols were developed as part of the Uniform Methods Project (UMP), funded by the U.S. Department of Energy (DOE). The principal objective for the project was to establish easy-to-follow protocols based on commonly accepted methods for a core set of widely deployed energy efficiency measures.

  18. Individual differences in the production of word classes in eight specific language-impaired preschoolers.

    Science.gov (United States)

    Le Normand, M T; Chevrie-Muller, C

    1991-01-01

    The production of word classes in eight 53-62-month-old specific language-impaired (SLI) children was described and compared with that of 30 normal 24-33-month-old children in the same play situation. SLI subjects and nonimpaired children were selected within specified mean length of utterance ranges (low MLU versus high MLU). Production of word classes by subjects was evaluated in order to determine (1) whether SLI children showed a similar or a different word-class profile among themselves and when compared with non-impaired children and (2) whether MLU related to word classes would be useful as a single clinical index in assessment of language acquisition. Results showed that scores of SLI children in production of word classes reflect important individual differences among subjects. In the high-MLU sample, all SLI children produced each word class relatively within the same range as the nonimpaired group. In the low-MLU sample two SLI children were very different in their word-class profile and individual differences were further confirmed by a discriminant function analysis. Correlations between MLU and word classes were significant in nonimpaired children for all variables except Questions and Onomatopoeia and were only significant in SLI children for Verbs, Prepositions, and Personal Pronouns. Such findings contribute support to the view that there is "deviant" pattern of language in SLI children and once again questions whether MLU is one of the best discriminating indicators to use in the clinical assessment of language organization.

  19. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells

    International Nuclear Information System (INIS)

    Woodrick, Jordan; Gupta, Suhani; Khatkar, Pooja; Dave, Kalpana; Levashova, Darya; Choudhury, Sujata; Elias, Hadi; Saha, Tapas; Mueller, Susette; Roy, Rabindra

    2015-01-01

    Highlights: • A method of monitoring lesion-specific recruitment of proteins in vivo is described. • Recruitment of repair enzymes to abasic sites is monitored by co-localization. • Repair protein recruitment is consistent with known protein–protein relationships. • Cells demonstrated complete repair of abasic sites by 90 min. - Abstract: DNA–protein relationships have been studied by numerous methods, but a particular gap in methodology lies in the study of DNA adduct-specific interactions with proteins in vivo, which particularly affects the field of DNA repair. Using the repair of a well-characterized and ubiquitous adduct, the abasic (AP) site, as a model, we have developed a comprehensive method of monitoring DNA lesion-specific recruitment of proteins in vivo over time. We utilized a surrogate system in which a Cy3-labeled plasmid containing a single AP-site was transfected into cells, and the interaction of the labeled DNA with BER enzymes, including APE1, Polβ, LIG1, and FEN1, was monitored by immunofluorescent staining of the enzymes by Alexafluor-488-conjugated secondary antibody. The recruitment of enzymes was characterized by quantification of Cy3-Alexafluor-488 co-localization. To validate the microscopy-based method, repair of the transfected AP-site DNA was also quantified at various time points post-transfection using a real time PCR-based method. Notably, the recruitment time kinetics for each enzyme were consistent with AP-site repair time kinetics. This microscopy-based methodology is reliable in detecting the recruitment of proteins to specific DNA substrates and can be extended to study other in vivo DNA–protein relationships in any DNA sequence and in the context of any DNA structure in transfectable proliferating or quiescent cells. The method may be applied to a variety of disciplines of nucleic acid transaction pathways, including repair, replication, transcription, and recombination

  20. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells

    Energy Technology Data Exchange (ETDEWEB)

    Woodrick, Jordan; Gupta, Suhani; Khatkar, Pooja; Dave, Kalpana; Levashova, Darya; Choudhury, Sujata; Elias, Hadi; Saha, Tapas; Mueller, Susette; Roy, Rabindra, E-mail: rr228@georgetown.edu

    2015-05-15

    Highlights: • A method of monitoring lesion-specific recruitment of proteins in vivo is described. • Recruitment of repair enzymes to abasic sites is monitored by co-localization. • Repair protein recruitment is consistent with known protein–protein relationships. • Cells demonstrated complete repair of abasic sites by 90 min. - Abstract: DNA–protein relationships have been studied by numerous methods, but a particular gap in methodology lies in the study of DNA adduct-specific interactions with proteins in vivo, which particularly affects the field of DNA repair. Using the repair of a well-characterized and ubiquitous adduct, the abasic (AP) site, as a model, we have developed a comprehensive method of monitoring DNA lesion-specific recruitment of proteins in vivo over time. We utilized a surrogate system in which a Cy3-labeled plasmid containing a single AP-site was transfected into cells, and the interaction of the labeled DNA with BER enzymes, including APE1, Polβ, LIG1, and FEN1, was monitored by immunofluorescent staining of the enzymes by Alexafluor-488-conjugated secondary antibody. The recruitment of enzymes was characterized by quantification of Cy3-Alexafluor-488 co-localization. To validate the microscopy-based method, repair of the transfected AP-site DNA was also quantified at various time points post-transfection using a real time PCR-based method. Notably, the recruitment time kinetics for each enzyme were consistent with AP-site repair time kinetics. This microscopy-based methodology is reliable in detecting the recruitment of proteins to specific DNA substrates and can be extended to study other in vivo DNA–protein relationships in any DNA sequence and in the context of any DNA structure in transfectable proliferating or quiescent cells. The method may be applied to a variety of disciplines of nucleic acid transaction pathways, including repair, replication, transcription, and recombination.

  1. Study of specific pharmacological activity of standardized composition of bee product substances for treatment of urogenital system

    Directory of Open Access Journals (Sweden)

    V. M. Koval

    2017-10-01

    Full Text Available Introduction. The work presents review of the data literature, which indicate the prospects of creation new highly efficient drugs for the treatment of chronic prostatitis and prostate gland adenoma based bioactive standardized substances of bee products, including powdered honey (PH, propolis phenolic hydrophobic drug (PPHD and bee pollen (BP. Materials and methods. Results of discussion of preclinical pharmacological studies of standardized substances of bee products composition – PH, PPHD and BP for the treatment of specified pathology is given in the experimental part. Results. It was found that the most pronounced anti-inflammatory effect on the level 40 % the mixture of APIs (PH, PPHD and BP detects at a dose of 100 mg/kg in relation to the reference drug – trianom capsules in doses of 100 and 130 mg/kg. Conclusions. Usage of bee products is grounded in creation of the new drug on their basis in the form of standardized substances of bee products composition –PH, PPHD and BP for chronic prostatitis and prostate gland adenoma. It was found that the most pronounced anti-inflammatory effect on 40 % the mixture of APIs (PH, PPHD and BP detects at a dose of 100 mg/kg. It was established that the composition of standard substances of bee products – PH, PPHD and BP at a dose of 100 mg/kg shows a more pronounced specific pharmacological effect in comparison to the reference product – trianom capsules at doses of 100 and 130 mg/kg, which positively affect the course of the pilot prostatitis in male rats caused by dichloroethyl.

  2. Production of specific-structured lipids by enzymatic interesterification: elucidation of acyl migration by response surface design

    DEFF Research Database (Denmark)

    Xu, Xuebing; Skands, Anja; Høy, Carl-Erik

    1998-01-01

    Production of specific-structured lipids (SSL) by lipase-catalyzed interesterification has been attracting more and more attention recently. However, it was found that acyl migration occurs during the reaction and causes the production of by-products. In this paper, the elucidation of acyl...

  3. Analysis of Formal Methods for Specification of E-Commerce Applications

    Directory of Open Access Journals (Sweden)

    Sadiq Ali Khan

    2016-01-01

    Full Text Available E-commerce based application characteristics portray elevated dynamics while incorporating decentralized nature. Extreme emphasis influencing structural design plus implementation, positions such applications highly appreciated. Significant research articles reveal that, applying formal methods addressing challenges incumbent with E-commerce based applications, contribute towards reliability and robustness obliging the system. Anticipating and designing sturdy e-process and concurrent implementation, allows application behavior extra strength against errors, frauds and hacking, minimizing program faults during application operations. Programmers find extreme difficulty guaranteeing correct processing under all circumstances, however, not impossible. Concealed flaws and errors, triggered only under unexpected and unanticipated scenarios, pilot subtle mistakes and appalling failures. Code authors utilize various formal methods for reducing these flaws. Mentioning prominent methods would include, ASM (Abstract State Machines, B-Method, z-Language, UML (Unified Modelling Language etc. This paper primarily focuses different formal methods applied while deliberating specification and verification techniques for cost effective.

  4. Impact of constraints and rules of user-involvement methods for IS concept creation and specification

    DEFF Research Database (Denmark)

    Jensen, Mika Yasuoka; Ohno, Takehiko; Nakatani, Momoko

    2015-01-01

    ideas. In this paper, by exemplifying our user-involvement method with game elements, ICT Service Design Game, in comparison with conventional brainstorming, we show the impact of constraints and rules in user-involvement methods when creating service concepts and specifications for information systems....... The analysis is based on a comparative experiment on two design methods and shows that the constraints and rules of our game approach fostered innovative idea generation in spite of participants’ limited knowledge of and experience with design processes. Although our analysis is still in a preliminary stage......, it indicates some positive impact of constraints and rules in design methods, especially when the methods are used by non-design professionals....

  5. New chromatographic method for separating Omeprazole from its degradation components and the quantitatively determining it in its pharmaceutical products

    International Nuclear Information System (INIS)

    Touma, M.; Rajab, A.; Seuleiman, M.

    2007-01-01

    New chromatographic method for Quantitative Determination of Omeprazole in its Pharmaceutical Products was produced. Omeprazole and its degradation components were well separated in same chromatogram by using high perfume liquid chromatography (HPLC). The new analytical method has been validated by these characteristic tests (accuracy, precision, range, linearity, specificity/selectivity, limit of detection (LOD) and limit of quantitative (LOQ) ).(author)

  6. New chromatographic Methods for Separation of Lansoprazole from its Degradation Components and The Quantitative Determination in its Pharmaceutical Products

    International Nuclear Information System (INIS)

    Touma, M.; Rajab, A.

    2009-01-01

    New chromatographic method was found for Quantitative Determination of Lansoprazole in its pharmaceutical products. Lansoprazole and its degradation components were well separated in same chromatogram by using high perfume liquid chromatography (HPLC). The new analytical method has been validated by these characteristic tests (accuracy, precision, range, linearity, specificity/selectivity, limit of detection (LOD) and limit of quantitative (LOQ)). (author)

  7. Fission product separation from seawater by electrocoagulation method

    International Nuclear Information System (INIS)

    Kitagaki, T.; Hoshino, T.; Sambommatsu, Y.; Yano, K.; Takeuchi, M.; Igarashi, T.; Suzuki, T.

    2013-01-01

    At the Fukushima Daiichi nuclear power station, seawater was urgently injected into the reactor core. Therefore a large amount of seawater containing highly radioactive fission products (FP) accumulated and its treatment has been a serious problem. FP such as Cs, Sr and I in water are generally removed by an ion exchanger such as zeolite and separated with column or chemical precipitation methods. An alternative electrocoagulation method, which efficiently separates fine particles from the liquid phase without a chemical reagent is expected to be part of a useful separation system that can reduce the amount of waste, decrease processing time and simplify the process. In this study, powdered adsorbents, such as ferrocyanide and zeolite, were added to seawater containing simulated FP, and the electrocoagulation effect with Al alloy electrodes were investigated. More than 99 % of Cs and 90 % of I were removed by potassium nickel hexacyanoferrate(II) and silver zeolite, respectively. Sedimentation was promoted by electrocoagulation and addition of an inorganic cohesion promoter further increased the sedimentation rate. Moreover, rapid dissolution reaction with heating of the aggregation substance was not observed, so the thermal risk of aqueous processing of it would be low. In addition, thermal analyses showed that the electrocoagulation process did not lead to thermal decomposition. Therefore, if the electrocoagulation method is applied to a decontamination system, it has the potential to thermally stabilize and reduce waste. (author)

  8. Progress in the methods of steel-production

    International Nuclear Information System (INIS)

    Onodera, S.

    1979-01-01

    The steel making and ingot making processes have made a great progress in the past two decades, which has been one of the direct supports for developing those megalo-technologies as in iron and steel making, petrochemical, fossil and nuclear power generation, and other industries. In discussing steel quality, large or small, everything is to the ingot, and in order to reduce miscellaneous imperfections in steel ingots, for largest ones in particular, tremendous efforts have been made so far. In addition to the improvements in conventional ingot making method, a number of innovational techniques were developed such as ESR, PESC, ABR, MHKW, BEST, etc. with some results in respective fields of application. Nevertheless, for largest sizes of ingot from 250 to 500 tons, the conventional method was successful enough. The history of conventional method is summarized for the past quarter century. The improved quality of large ingots could bring some more merits in the final forged products, which are: 1) Improved isotropy of mechanical properties, 2) sufficient effect of forging with much less forging ratio. (orig./HJ) [de

  9. Reduction in requirements for allogeneic blood products: nonpharmacologic methods.

    Science.gov (United States)

    Hardy, J F; Bélisle, S; Janvier, G; Samama, M

    1996-12-01

    Various strategies have been proposed to decrease bleeding and allogeneic transfusion requirements during and after cardiac operations. This article attempts to document the usefulness, or lack thereof, of the nonpharmacologic methods available in clinical practice. Blood conservation methods were reviewed in chronologic order, as they become available to patients during the perisurgical period. The literature in support of or against each strategy was reexamined critically. Avoidance of preoperative anemia and adherence to published guidelines for the practice of transfusion are of paramount importance. Intraoperatively, tolerance of low hemoglobin concentrations and use of autologous blood (predonated or harvested before bypass) will reduce allogeneic transfusions. The usefulness of plateletpheresis and retransfusion of shed mediastinal fluid remains controversial. Intraoperatively and postoperatively, maintenance of normothermia contributes to improved hemostasis. Several approaches have been shown to be effective. An efficient combination of methods can reduce, and sometimes abolish, the need for allogeneic blood products after cardiac operations, inasmuch as all those involved in the care of cardiac surgical patients adhere thoughtfully to existing transfusion guidelines.

  10. Use of basic and specific pre-treatments for the biogas production. Revision and analysis

    International Nuclear Information System (INIS)

    Martínez Hernández, Carlos M.; García López, Yaser

    2016-01-01

    Keeping in mind the importance that has today the use of national and international biogas plants, which use as raw material animal manure; as well as agricultural and agroindustrial residuals for the energy production and biofertilizers, minimizing the aspects of environmental contamination. This work is presented in three parts, in those which: firstly the technologies and the methods are described to apply the basic and special pre-treatments to the different biomass in order to obtain their maximum potential of methane. A second part where it is approached the particularities to do that. Finally, a third part where their possible use is analyzed in the Cuban case. As a result of the same one, the state of the art is shown in the use of basic and special pre-treatments, with the objective of to potentialize the increase of the methane production in agricultural or animal biomasses. (author)

  11. Discriminant validity, responsiveness and reliability of the arthritis-specific Work Productivity Survey assessing workplace and household productivity in patients with psoriatic arthritis

    Science.gov (United States)

    2014-01-01

    Introduction The novel arthritis-specific Work Productivity Survey (WPS) was developed to estimate patient productivity limitations associated with arthritis within and outside the home, which is an unmet need in psoriatic arthritis (PsA). The WPS has been validated in rheumatoid arthritis. This report assesses the discriminant validity, responsiveness and reliability of the WPS in adult-onset PsA. Methods Psychometric properties were assessed using data from the RAPID-PsA trial (NCT01087788) investigating certolizumab pegol (CZP) efficacy and safety in PsA. WPS was completed at baseline and every 4 weeks until Week 24. Validity was evaluated at baseline via known-groups defined using first and third quartiles of patients’ Disease Activity Score 28 based on C-reactive protein (DAS28(CRP)), Health Assessment Questionnaire-Disability Index (HAQ-DI), Short Form-36 (SF-36) items and PsA Quality of Life (PsAQoL) scores. Responsiveness and reliability were assessed by comparing WPS mean changes at Week 12 in American College of Rheumatology 20% improvement criteria (ACR20) or HAQ-DI Minimal Clinically Important Difference (MCID) 0.3 responders versus non-responders, as well as using standardized response means (SRM). All comparisons were conducted on the observed cases in the Randomized Set, regardless of the randomization group, using a non-parametric bootstrap-t method. Results Compared with patients with a better health state, patients with a worse health state had on average 2 to 6 times more household work days lost, more days with reduced household productivity, more days missed of family/social/leisure activities, more days with outside help hired and a significantly higher interference of arthritis per month. Among employed patients, those with a worse health state had 2 to 4 times more workplace days lost, more days with patient workplace productivity reduced, and a significantly higher interference of arthritis on patient workplace productivity versus

  12. The production of fully deacetylated chitosan by compression method

    Directory of Open Access Journals (Sweden)

    Xiaofei He

    2016-03-01

    Full Text Available Chitosan’s activities are significantly affected by degree of deacetylation (DDA, while fully deacetylated chitosan is difficult to produce in a large scale. Therefore, this paper introduces a compression method for preparing 100% deacetylated chitosan with less environmental pollution. The product is characterized by XRD, FT-IR, UV and HPLC. The 100% fully deacetylated chitosan is produced in low-concentration alkali and high-pressure conditions, which only requires 15% alkali solution and 1:10 chitosan powder to NaOH solution ratio under 0.11–0.12 MPa for 120 min. When the alkali concentration varied from 5% to 15%, the chitosan with ultra-high DDA value (up to 95% is produced.

  13. Methods and Algorithms for Economic MPC in Power Production Planning

    DEFF Research Database (Denmark)

    Sokoler, Leo Emil

    in real-time. A generator can represent a producer of electricity, a consumer of electricity, or possibly both. Examples of generators are heat pumps, electric vehicles, wind turbines, virtual power plants, solar cells, and conventional fuel-fired thermal power plants. Although this thesis is mainly...... concerned with EMPC for minutes-ahead production planning, we show that the proposed EMPC scheme can be extended to days-ahead planning (including unit commitment) as well. The power generation from renewable energy sources such as wind and solar power is inherently uncertain and variable. A portfolio...... design an algorithm based on the alternating direction method of multipliers (ADMM) to solve input-constrained OCPs with convex objective functions. The OCPs that occur in EMPC of dynamically decoupled subsystems, e.g. power generators, have a block-angular structure. Subsystem decomposition algorithms...

  14. Method of removing deterioration product in hydrocarbon type solvent

    International Nuclear Information System (INIS)

    Ito, Yoshifumi; Takashina, Toru; Murasawa, Kenji.

    1988-01-01

    Purpose: To remarkably reduce radioactive wastes by bringing adsorbents comprising titanium oxide and/or zirconium oxide into contact with hydrocarbon type solvents. Method: In a nuclear fuel re-processing step, an appropriate processing is applied to extraction solvents suffering from radioactive degradation, to separate the hydrocarbon solvents and store them in a solvent tank. Then, titanium oxide and/or zirconium oxide adsorbents are continuously mixed and agitated therewith to adsorb degradation products on the adsorbents. Then, they are introduced with adsorbent separators to recover purified hydrocarbon type solvents. Meanwhile, the separated adsorbents are discharged from pipeways. This enables to regenerate the hydrocarbon type solvents for reuse, as well as remarkably reduce the radioactive wastes. (Takahashi, M.)

  15. Compound specific radiocarbon content of lignin oxidation products from the Altamaha river and Coastal Georgia

    Energy Technology Data Exchange (ETDEWEB)

    Culp, Randy, E-mail: rculp@uga.edu [Center for Applied Isotope Studies, University of Georgia, Athens, Georgia (United States)

    2013-01-15

    Compound-specific isotope analysis (CSIA) is a powerful tool in organic geochemistry by providing detailed information about an individual organic compound's history with regard to its source and process of formation. Most CSIA involves measurement of the stable isotope ratio of carbon ({sup 13}C/{sup 12}C) and hydrogen (D/H) following separation by gas or liquid chromatography. New applications are being developed using compound-specific radiocarbon ({sup 14}C) content for delineating age of materials, rates of decomposition and residence time in various environments. This paper details the isotopic work on specific lignin monomers derived from terrestrial plants transported and deposited within the Altamaha River, estuary and off-shore Georgia in the Atlantic Ocean. By using gas chromatographic separation and identification of selected lignin oxidation products (LOP), the harvesting of these compounds using preparative fraction collection, and measurement of their {sup 14}C content using accelerator mass spectrometry, details of the age and presence of specific biomarkers unique to a given terrestrial source are revealed. Radiocarbon ages determined from water-column particulate organic carbon and sediment LOPs indicate a range of ages from modern to well over 5,000 years for the former and latter respectively. Transport mechanisms and particle size associations on mineral grains may play a significant role in {sup 14}C distribution in estuary and near-shore coastal environments. This data indicates higher than modern {sup 14}C activities in large particle-size sediment fractions in contrast to older LOP {sup 14}C ages found associated with the same coarse grain sediments. Individual LOP ages substantiate older terrestrial materials persist in the off-shore environment even though in the presence of modern marine {sup 14}C sources.

  16. Compound specific radiocarbon content of lignin oxidation products from the Altamaha river and Coastal Georgia

    International Nuclear Information System (INIS)

    Culp, Randy

    2013-01-01

    Compound-specific isotope analysis (CSIA) is a powerful tool in organic geochemistry by providing detailed information about an individual organic compound’s history with regard to its source and process of formation. Most CSIA involves measurement of the stable isotope ratio of carbon ( 13 C/ 12 C) and hydrogen (D/H) following separation by gas or liquid chromatography. New applications are being developed using compound-specific radiocarbon ( 14 C) content for delineating age of materials, rates of decomposition and residence time in various environments. This paper details the isotopic work on specific lignin monomers derived from terrestrial plants transported and deposited within the Altamaha River, estuary and off-shore Georgia in the Atlantic Ocean. By using gas chromatographic separation and identification of selected lignin oxidation products (LOP), the harvesting of these compounds using preparative fraction collection, and measurement of their 14 C content using accelerator mass spectrometry, details of the age and presence of specific biomarkers unique to a given terrestrial source are revealed. Radiocarbon ages determined from water-column particulate organic carbon and sediment LOPs indicate a range of ages from modern to well over 5,000 years for the former and latter respectively. Transport mechanisms and particle size associations on mineral grains may play a significant role in 14 C distribution in estuary and near-shore coastal environments. This data indicates higher than modern 14 C activities in large particle-size sediment fractions in contrast to older LOP 14 C ages found associated with the same coarse grain sediments. Individual LOP ages substantiate older terrestrial materials persist in the off-shore environment even though in the presence of modern marine 14 C sources.

  17. Hydrocarbon productivities in different Botryococcus strains: comparative methods in product quantification.

    Science.gov (United States)

    Eroglu, Ela; Okada, Shigeru; Melis, Anastasios

    2011-08-01

    Six different strains of the green microalgae Botryococcus belonging to the A-race or B-race, accumulating alkadiene or botryococcene hydrocarbons, respectively, were compared for biomass and hydrocarbon productivities. Biomass productivity was assessed gravimetrically upon strain growth in the laboratory under defined conditions. Hydrocarbon productivities were measured by three different and independent experimental approaches, including density equilibrium of the intact cells and micro-colonies, spectrophotometric analysis of hydrocarbon extracts, and gravimetric quantitation of eluted hydrocarbons. All three hydrocarbon-quantitation methods yielded similar results for each of the strains examined. The B-race microalgae Botryococcus braunii var. Showa and Kawaguchi-1 constitutively accumulated botryococcene hydrocarbons equivalent to 30% and 20%, respectively, of their overall biomass. The A-race microalgae Botryococcus braunii, varieties Yamanaka, UTEX 2441 and UTEX LB572 constitutively accumulated alkadiene hydrocarbons ranging from 14% to 13% and 10% of their overall biomass, respectively. Botryococcus sudeticus (UTEX 2629), a morphologically different green microalga, had the lowest hydrocarbon accumulation, equal to about 3% of its overall biomass. Results validate the density equilibrium and spectrophotometric analysis methods in the quantitation of botryococcene-type hydrocarbons. These analytical advances will serve in the screening and selection of B. braunii and of other microalgae in efforts to identify those having a high hydrocarbon content for use in commercial applications.

  18. Generating quantitative models describing the sequence specificity of biological processes with the stabilized matrix method

    Directory of Open Access Journals (Sweden)

    Sette Alessandro

    2005-05-01

    Full Text Available Abstract Background Many processes in molecular biology involve the recognition of short sequences of nucleic-or amino acids, such as the binding of immunogenic peptides to major histocompatibility complex (MHC molecules. From experimental data, a model of the sequence specificity of these processes can be constructed, such as a sequence motif, a scoring matrix or an artificial neural network. The purpose of these models is two-fold. First, they can provide a summary of experimental results, allowing for a deeper understanding of the mechanisms involved in sequence recognition. Second, such models can be used to predict the experimental outcome for yet untested sequences. In the past we reported the development of a method to generate such models called the Stabilized Matrix Method (SMM. This method has been successfully applied to predicting peptide binding to MHC molecules, peptide transport by the transporter associated with antigen presentation (TAP and proteasomal cleavage of protein sequences. Results Herein we report the implementation of the SMM algorithm as a publicly available software package. Specific features determining the type of problems the method is most appropriate for are discussed. Advantageous features of the package are: (1 the output generated is easy to interpret, (2 input and output are both quantitative, (3 specific computational strategies to handle experimental noise are built in, (4 the algorithm is designed to effectively handle bounded experimental data, (5 experimental data from randomized peptide libraries and conventional peptides can easily be combined, and (6 it is possible to incorporate pair interactions between positions of a sequence. Conclusion Making the SMM method publicly available enables bioinformaticians and experimental biologists to easily access it, to compare its performance to other prediction methods, and to extend it to other applications.

  19. Development and Evaluation of Event-Specific Quantitative PCR Method for Genetically Modified Soybean MON87701.

    Science.gov (United States)

    Tsukahara, Keita; Takabatake, Reona; Masubuchi, Tomoko; Futo, Satoshi; Minegishi, Yasutaka; Noguchi, Akio; Kondo, Kazunari; Nishimaki-Mogami, Tomoko; Kurashima, Takeyo; Mano, Junichi; Kitta, Kazumi

    2016-01-01

    A real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) soybean event, MON87701. First, a standard plasmid for MON87701 quantification was constructed. The conversion factor (C f ) required to calculate the amount of genetically modified organism (GMO) was experimentally determined for a real-time PCR instrument. The determined C f for the real-time PCR instrument was 1.24. For the evaluation of the developed method, a blind test was carried out in an inter-laboratory trial. The trueness and precision were evaluated as the bias and reproducibility of relative standard deviation (RSDr), respectively. The determined biases and the RSDr values were less than 30 and 13%, respectively, at all evaluated concentrations. The limit of quantitation of the method was 0.5%, and the developed method would thus be applicable for practical analyses for the detection and quantification of MON87701.

  20. Waste acceptance product specifications for vitrified high-level waste forms

    International Nuclear Information System (INIS)

    Applewhite-Ramsey, A.; Sproull, J.F.

    1993-01-01

    The Nuclear Waste Policy Act of 1982 mandated that all high-level waste (HLW) be sent to a federal geologic repository for permanent disposal. DOE published the Environmental Assessment in 1982 which identified borosilicate glass as the chosen HLW form. 1 In 1985 the Department of Energy instituted a Waste Acceptance Process to assure that DWPF glass waste forms would be acceptable to such a repository. This assurance was important since production of waste forms will precede repository construction and licensing. As part of this Waste Acceptance Process, the DOE Office of Civilian Radioactive Waste Management (RW) formed the Waste Acceptance Committee (WAC). The WAC included representatives from the candidate repository sites, the waste producing sites and DOE. The WAC was responsible for developing the Waste Acceptance Preliminary Specifications (WAPS) which defined the requirements the waste forms must meet to be compatible with the candidate repository geologies

  1. Specificities of micro-structured reactors for hydrogen production and purification

    Energy Technology Data Exchange (ETDEWEB)

    Dupont, N.; Germani, G.; Van Veen, A.C.; Schuurman, Y.; Mirodatos, C. [Institut de Recherches sur la Catalyse - CNRS, 2, Avenue Albert Einstein, 69626 Villeurbanne Cedex (France); Schaefer, G. [Atotech Deutschland GmbH, PO Box 210780, 10507 Berlin (Germany)

    2007-07-15

    This paper presents the specificities of micro-structured reactors as compared to conventional fixed-bed reactors through two case studies devoted to (i) hydrogen production by methanol steam reforming, (ii) hydrogen purification by water-gas shift (WGS). Key features like catalyst coating stability, temperature and pressure management, effects of operating conditions (residence time, pressure drops, etc.) are well identified as controlling the micro-reactor performances for methanol reforming. These devices are also shown to be excellent tools for fast access to reaction kinetics as exemplified for the WGS reaction, subject to operating conditions carefully chosen to ensure proper hydrodynamics, in order to use conventional plug flow reactor models for extracting rate constants. (author)

  2. Specification and Verification of Distributed Embedded Systems: A Traffic Intersection Product Family

    Directory of Open Access Journals (Sweden)

    José Meseguer

    2010-09-01

    Full Text Available Distributed embedded systems (DESs are no longer the exception; they are the rule in many application areas such as avionics, the automotive industry, traffic systems, sensor networks, and medical devices. Formal DES specification and verification is challenging due to state space explosion and the need to support real-time features. This paper reports on an extensive industry-based case study involving a DES product family for a pedestrian and car 4-way traffic intersection in which autonomous devices communicate by asynchronous message passing without a centralized controller. All the safety requirements and a liveness requirement informally specified in the requirements document have been formally verified using Real-Time Maude and its model checking features.

  3. Energy-Extended CES Aggregate Production: Current Aspects of Their Specification and Econometric Estimation

    Directory of Open Access Journals (Sweden)

    Paul E. Brockway

    2017-02-01

    Full Text Available Capital–labour–energy Constant Elasticity of Substitution (CES production functions and their estimated parameters now form a key part of energy–economy models which inform energy and emissions policy. However, the collation and guidance as to the specification and estimation choices involved with such energy-extended CES functions is disparate. This risks poorly specified and estimated CES functions, with knock-on implications for downstream energy–economic models and climate policy. In response, as a first step, this paper assembles in one place the major considerations involved in the empirical estimation of these CES functions. Discussions of the choices and their implications lead to recommendations for CES empiricists. The extensive bibliography allows those interested to dig deeper into any aspect of the CES parameter estimation process.

  4. Improving the Management of Innovative Development of Industrial Production According to Industry Specifics

    Directory of Open Access Journals (Sweden)

    Papizh Yuliia S.

    2018-03-01

    Full Text Available The problem of improvement of management efficiency of innovative development of industrial enterprises in modern economic conditions is indicated. The dynamics of innovative processes in Ukraine together with volumes of innovative activity of domestic enterprises are analyzed. The basic principles of formation and efficient functioning of the organizational-economic mechanism of innovative development of industrial production are substantiated. The branch specificity in management of innovative development of coal enterprises is identified. Directions of improvement of the organizational-economic mechanism of management of innovative development of enterprises of coal industry are defined. The basic principles of introduction of the mechanism for stimulation of innovative development of enterprises of coal industry are suggested.

  5. Environmentally-friendly product development: methods and tools

    National Research Council Canada - National Science Library

    Abele, Eberhard; Anderl, R; Birkhofer, Herbert

    2005-01-01

    ... to assess a product's environmental effects. Fig. 1. Vision of Environment as a key target for product development vvi Preface Product related environmental issues are getting more and more political and public awareness. Development of environmentally friendly products has become an action item for both, politics and industry (UNFCCC 1997). Energy...

  6. Radioimmunoassay (RIA), a highly specific, extremely sensitive quantitative method of analysis

    Energy Technology Data Exchange (ETDEWEB)

    Strecker, H; Hachmann, H; Seidel, L [Farbwerke Hoechst A.G., Frankfurt am Main (Germany, F.R.). Radiochemisches Lab.

    1979-02-01

    Radioimmunoassay is an analytical method combining the sensitivity of radioactivity measurements and the specificity of the antigen-antibody-reaction. Thus, substances can be measured in concentrations as low as picograms per milliliter serum besides a millionfold excess of otherwise disturbing material (for example in serum). The method is simple to perform and is at present mainly used in the field of endocrinology. Further areas of possible application are in the diagnosis of infectious disease, drug research, environmental protection, forensic medicine as well as general analytics. Quantities of radioactivity, exclusively used in vitro, are in the nano-Curie range. Therefore the radiation dose is negligible.

  7. Statistical methods for site-specific analysis of cancer among the A-bomb survivors

    International Nuclear Information System (INIS)

    Pierce, D.A.; Preston, D.L.

    1992-01-01

    Statistical methods are presented for joint, or simultaneous, analysis of the risks of several types of cancer for the A-bomb survivors. Previous analyses have been made either for all cancers except leukemia together, or have been done separately by cancer type. Either of these approaches has serious limitations, and the aim of joint analysis is to overcome these, while taking advantage of the strengths of each. The primary advantage of joint analysis is that models for risks of various cancer types can have some parameters in common, and others which are type-specific. This serves to overcome difficulties due to the limited data on specific cancer types. It also provides for significant tests comparing both type-specific risks and type-specific effects of modifying factors such as sex and age. These methods are exemplified here by joint analysis of three classes of cancer considered by the BEIR-V committee: (i) respiratory, (ii) digestive, and (iii) other cancers, excluding leukemia and breast cancer. The primary aim is to illustrate the general advantages of joint analyses, but in addition some comparison is made between the results of such joint analyses and the conclusions drawn by BEIR-V committee from separate analyses. (author)

  8. Testing a Novel Method to Approximate Wood Specific Gravity of Trees

    Science.gov (United States)

    Michael C. Wiemann; G. Bruce. Williamson

    2012-01-01

    Wood specific gravity (SG) has long been used by foresters as an index for wood properties. More recently, SG has been widely used by ecologists as a plant functional trait and as a key variable in estimates of biomass. However, sampling wood to determine SG can be problematic; at present, the most common method is sampling with an increment borer to extract a bark-to-...

  9. Status of JENDL High Energy File. Evaluation method, tools, specification, release procedure, etc

    Energy Technology Data Exchange (ETDEWEB)

    Fukahori, Tokio [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    1998-11-01

    The ENDF-6 format file should be kept as a standard distribution file and it is not difficult to convert into some other form for code`s libraries. From this point of view, status of JENDL High Energy File is introduced in this report as well as evaluation strategy, recommended specification, stored nuclides and quantities, a format structure, evaluation methods and tools, and release plan. (author)

  10. Reference neutron radiations. Part 1: Characteristics and methods of production

    International Nuclear Information System (INIS)

    2001-01-01

    ISO 8529 consists of the following parts, under the general title Reference neutron radiations: Part 1: Characteristics and methods of production; Part 2: Calibration fundamentals of radiation protection devices related to the basic quantities characterizing the radiation field; Part 3: Calibration of area and personal dosimeters and determination of response as a function of energy and angle of incidence. This Part 1. of ISO 8529 specifies the reference neutron radiations, in the energy range from thermal up to 20 MeV, for calibrating neutron-measuring devices used for radiation protection purposes and for determining their response as a function of neutron energy. Reference radiations are given for neutron fluence rates of up to 1x10 9 m 2 s-1 , corresponding, at a neutron energy of 1 MeV, to dose-equivalent rates of up to 100 mSv h -1 . This part of ISO 8529 is concerned only with the methods of producing and characterizing the neutron reference radiations. The procedures for applying these radiations for calibrations are described in ISO 8529-2 and ISO 8529-3. The reference radiations specified are the following: neutrons from radionuclide sources, including neutrons from sources in a moderator; neutrons produced by nuclear reactions with charged particles from accelerators; neutrons from reactors. In view of the methods of production and use of them, these reference radiations are divided, for the purposes of this part of ISO 8529, into the following two separate sections. In clause 4, radionuclide neutron sources with wide spectra are specified for the calibration of neutron measuring devices. These sources should be used by laboratories engaged in the routine calibration of neutron-measuring devices, the particular design of which has already been type tested. In clause 5, accelerator-produced monoenergetic neutrons and reactor-produced neutrons with wide or quasi monoenergetic spectra are specified for determining the response of neutron-measuring devices

  11. Development of an Analytic Method for Sulfur Compounds in Aged Garlic Extract with the Use of a Postcolumn High Performance Liquid Chromatography Method with Sulfur-Specific Detection.

    Science.gov (United States)

    Matsutomo, Toshiaki; Kodera, Yukihiro

    2016-02-01

    Garlic and its processed preparations contain numerous sulfur compounds that are difficult to analyze in a single run using HPLC. The aim of this study was to develop a rapid and convenient sulfur-specific HPLC method to analyze sulfur compounds in aged garlic extract (AGE). We modified a conventional postcolumn HPLC method by employing a hexaiodoplatinate reagent. Identification and structural analysis of sulfur compounds were conducted by LC-mass spectrometry (LC-MS) and nuclear magnetic resonance. The production mechanisms of cis-S-1-propenylcysteine (cis-S1PC) and S-allylmercaptocysteine (SAMC) were examined by model reactions. Our method has the following advantages: less interference from nonsulfur compounds, high sensitivity, good correlation coefficients (r > 0.98), and high resolution that can separate >20 sulfur compounds, including several isomers, in garlic preparations in a single run. This method was adapted for LC-MS analysis. We identified cis-S1PC and γ-glutamyl-S-allyl-mercaptocysteine in AGE. The results of model reactions suggest that cis-S1PC is produced from trans-S1PC through an isomerization reaction and that SAMC is produced by a reaction involving S-allylcysteine/S1PC and diallyldisulfide during the aging period. We developed a rapid postcolumn HPLC method for both qualitative and quantitative analyses of sulfur compounds, and this method helped elucidate a potential mechanism of cis-S1PC and SAMC action in AGE. © 2016 American Society for Nutrition.

  12. Pan-specific MHC class I predictors: A benchmark of HLA class I pan-specific prediction methods

    DEFF Research Database (Denmark)

    Zhang, Hao; Lundegaard, Claus; Nielsen, Morten

    2009-01-01

    not previously been compared using independent evaluation sets. Results: A diverse set of quantitative peptide binding affinity measurements was collected from IEDB, together with a large set of HLA class I ligands from the SYFPEITHI database. Based on these data sets, three different pan-specific HLA web...

  13. Thermal degradation characteristics and products obtained after pyrolysis of specific polymers found in Waste Electrical and Electronic Equipment

    Institute of Scientific and Technical Information of China (English)

    Evangelia C.Vouvoudi; Aristea T.Rousi; Dimitris S.Achilias

    2017-01-01

    Modern societies strongly support the recycling practices over simple waste accumulation due to environmental harm caused.In the framework of sustainable recycling of plastics from WEEE,pyrolysis is proposed here as a means of obtaining secondary value-added products.The aim of this study was to investigate the thermal degradation and the products obtained after pyrolysis of specific polymers found in the plastic part of WEEE,using thermogravimetric analysis and a pyrolizer equipped with a GC/MS.Polymers studied include ABS,HIPS,PC and a blend having a composition similar to that appearing in WEEE.It was found that,PC shows greater heat endurance compared to the other polymers,whereas ABS depolymerizes in three-steps.The existence of several polymers in the blend results in synergistic effects which decrease the onset and final temperature of degradation.Moreover,the fragmentation occurred in the pyrolyzer,at certain temperatures,resulted in a great variety of compounds,depending on the polymer type,such as monomers,aromatic products,phenolic compounds and hydrocarbons.The main conclusion from this investigation is that pyrolysis could be an effective method for the sustainable recycling of the plastic part of WEEE resulting in a mixture of chemicals with varying composition but being excellent to be used as fuel retrieved from secondary recycling sources.

  14. The specific localization of advanced glycation end-products (AGEs) in rat pancreatic islets.

    Science.gov (United States)

    Morioka, Yuta; Teshigawara, Kiyoshi; Tomono, Yasuko; Wang, Dengli; Izushi, Yasuhisa; Wake, Hidenori; Liu, Keyue; Takahashi, Hideo Kohka; Mori, Shuji; Nishibori, Masahiro

    2017-08-01

    Advanced glycation end-products (AGEs) are produced by non-enzymatic glycation between protein and reducing sugar such as glucose. Although glyceraldehyde-derived AGEs (Glycer-AGEs), one of the AGEs subspecies, have been reported to be involved in the pathogenesis of various age-relating diseases such as diabetes mellitus or arteriosclerosis, little is known about the pathological and physiological mechanism of AGEs in vivo. In present study, we produced 4 kinds of polyclonal antibodies against AGEs subspecies and investigated the localization of AGEs-modified proteins in rat peripheral tissues, making use of these antibodies. We found that Glycer-AGEs and methylglyoxal-derived AGEs (MGO-AGEs) were present in pancreatic islets of healthy rats, distinguished clearly into the pancreatic α and β cells, respectively. Although streptozotocin-induced diabetic rats suffered from remarkable impairment of pancreatic islets, the localization and deposit levels of the Glycer- and MGO-AGEs were not altered in the remaining α and β cells. Remarkably, the MGO-AGEs in pancreatic β cells were localized into the insulin-secretory granules. These results suggest that the cell-specific localization of AGEs-modified proteins are presence generally in healthy peripheral tissues, involved in physiological intracellular roles, such as a post-translational modulator contributing to the secretory and/or maturational functions of insulin. Copyright © 2017 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

  15. Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments.

    Science.gov (United States)

    Chenthamarakshan, Aiswarya; Parambayil, Nayana; Miziriya, Nafeesathul; Soumya, P S; Lakshmi, M S Kiran; Ramgopal, Anala; Dileep, Anuja; Nambisan, Padma

    2017-02-13

    Fungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As laccase production is highly dependent on medium components and cultural conditions, optimization of the same is essential for efficient product production. Production of laccase by fungal strain Marasmiellus palmivorus LA1 under solid state fermentation was optimized by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L8) was designed using Qualitek-4 software to study the interactions and relative influence of the seven selected factors by one factor at a time approach. The optimum condition formulated was temperature (28 °C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum concentration (number of mycelial agar pieces) (6Nos.) and substrate length (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. Statistical optimization leads to the elimination of an insignificant medium component ammonium dihydrogen phosphate from the process and contributes to a 1.06 fold increase in enzyme production. A final production of 667.4 ± 13 IU/mL laccase activity paves way for the application of this strain for industrial applications. Study optimized lignin degrading laccases from Marasmiellus palmivorus LA1. This laccases can thus be used for further applications in different scales of production after analyzing the properties of the enzyme. Study also confirmed the use of taguchi method for optimizations of product production.

  16. Stage- and gender-specific proteomic analysis of Brugia malayi excretory-secretory products.

    Directory of Open Access Journals (Sweden)

    Yovany Moreno

    Full Text Available INTRODUCTION: While we lack a complete understanding of the molecular mechanisms by which parasites establish and achieve protection from host immune responses, it is accepted that many of these processes are mediated by products, primarily proteins, released from the parasite. Parasitic nematodes occur in different life stages and anatomical compartments within the host. Little is known about the composition and variability of products released at different developmental stages and their contribution to parasite survival and progression of the infection. METHODOLOGY/PRINCIPAL FINDINGS: To gain a deeper understanding on these aspects, we collected and analyzed through 1D-SDS PAGE and LC-MS/MS the Excretory-Secretory Products (ESP of adult female, adult male and microfilariae of the filarial nematode Brugia malayi, one of the etiological agents of human lymphatic filariasis. This proteomic analysis led to the identification of 228 proteins. The list includes 76 proteins with unknown function as well as also proteins with potential immunoregulatory properties, such as protease inhibitors, cytokine homologues and carbohydrate-binding proteins. Larval and adult ESP differed in composition. Only 32 proteins were shared between all three stages/genders. Consistent with this observation, different gene ontology profiles were associated with the different ESP. CONCLUSIONS/SIGNIFICANCE: A comparative analysis of the proteins released in vitro by different forms of a parasitic nematode dwelling in the same host is presented. The catalog of secreted proteins reflects different stage- and gender-specific related processes and different strategies of immune evasion, providing valuable insights on the contribution of each form of the parasite for establishing the host-parasite interaction.

  17. 77 FR 47397 - Request for Nominations of Specific Drug/Biologic Product(s) That Could Be Brought Before the...

    Science.gov (United States)

    2012-08-08

    ... decision-making processes. Significant public health and safety issues are brought before these committees... future pediatric product development by focusing on products whose development would benefit the most...

  18. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products

    DEFF Research Database (Denmark)

    Ramos, Cintia Lacerda; Thorsen, Line; Schwan, Rosane Freitas

    2013-01-01

    A total of 234 LAB isolates from Brazilian food products were initially screened for their ability to survive at pH 2.0. Fifty one of the isolates survived and were selected. They were characterized by phenotypic methods, rep-PCR and identified using 16S rRNA gene sequencing as Lactobacillus ferm...... FFC199 showed adhesion ability to Caco-2 cells (1.6, 1.1 and 0.9 respectively) similar to the commercial probiotic, Lactobacillus rhamnosus GG (1.5. They were able to increase the transepithelial electrical resistance (TEER) of Caco-2 cells over 24 h (p ... that the probiotic characteristics were strain-specific and that the isolates L. plantarum CH3 and CH41 (cocoa) and L. brevis FFC199 (cauim) exhibited potential probiotics properties. (C) 2013 Elsevier Ltd. All rights reserved....

  19. Nuclear electronic equipment for control and monitoring panel. Specifications and methods for testing radiation detectors

    International Nuclear Information System (INIS)

    Roguin, Andre.

    1976-02-01

    This document will be of interest to users and makers of neutron and gamma radiations detectors in the field of nuclear reactor control and protection. Information is given which will enable users to optimize their choice and methods of using equipment, and makers to optimize their methods of fabrication. It should also serve as a model from which official specifications, technical instructions and test methods for these detectors, could be established. A detailed description is given of the appropriate parameters, terminology and notations. General specifications, operating conditions and test methods are indicated. The following detectors are studied: in-core detectors: fission ionization chambers, self powered neutron detectors (S.P.N.D.); out-core detectors: boron ionization chambers (for monitoring), boron trifluoride proportional counter tubes, boron lined proportional counter tubes, helium-3 proportional counter tubes. The devices described in the document are intended for industrial radiation monitoring applications and not for calibration standards (dosimetry) or for health physics measurement purposes. They are characterized by their fidelity, fast response, reliability and long lifetimes [fr

  20. Physically consistent data assimilation method based on feedback control for patient-specific blood flow analysis.

    Science.gov (United States)

    Ii, Satoshi; Adib, Mohd Azrul Hisham Mohd; Watanabe, Yoshiyuki; Wada, Shigeo

    2018-01-01

    This paper presents a novel data assimilation method for patient-specific blood flow analysis based on feedback control theory called the physically consistent feedback control-based data assimilation (PFC-DA) method. In the PFC-DA method, the signal, which is the residual error term of the velocity when comparing the numerical and reference measurement data, is cast as a source term in a Poisson equation for the scalar potential field that induces flow in a closed system. The pressure values at the inlet and outlet boundaries are recursively calculated by this scalar potential field. Hence, the flow field is physically consistent because it is driven by the calculated inlet and outlet pressures, without any artificial body forces. As compared with existing variational approaches, although this PFC-DA method does not guarantee the optimal solution, only one additional Poisson equation for the scalar potential field is required, providing a remarkable improvement for such a small additional computational cost at every iteration. Through numerical examples for 2D and 3D exact flow fields, with both noise-free and noisy reference data as well as a blood flow analysis on a cerebral aneurysm using actual patient data, the robustness and accuracy of this approach is shown. Moreover, the feasibility of a patient-specific practical blood flow analysis is demonstrated. Copyright © 2017 John Wiley & Sons, Ltd.

  1. A validated specific stability-indicating RP-HPLC assay method for Ambrisentan and its related substances.

    Science.gov (United States)

    Narayana, M B V; Chandrasekhar, K B; Rao, B M

    2014-09-01

    A validated specific stability-indicating reverse-phase liquid chromatographic method was developed for the quantitative determination of Ambrisentan as well as its related substances in bulk samples, pharmaceutical dosage forms in the presence of degradation products and its related impurities. Forced degradation studies were performed on bulk samples of Ambrisentan as per the ICH-prescribed stress conditions using acid, base, oxidative, thermal stress and photolytic degradation to show the stability-indicating power of the LC method. Significant degradation in acidic, basic stress conditions was observed and no degradation was observed in other stress conditions. The chromatographic method was optimized using the samples generated from the forced degradation studies and the impurity-spiked solution. Good resolution between the peaks corresponds to Ambrisentan-related impurities and degradation products from the analyte were achieved on a SunFire C18 column using a mobile phase consisting of a mixture of potassium dihydrogen orthophosphate at a pH adjusted to 2.5 with ortho-phosphoric acid in water and a mixture of acetonitrile:methanol using a simple linear gradient. The detection was carried out at 225 nm. The limit of detection and the limit of quantification for the Ambrisentan and its related impurities were established. The stressed test solutions were assayed against the qualified working standard of Ambrisentan and the mass balance in each case was between 98.9 and 100.3%, indicating that the developed LC method was stability indicating. Validation of the developed LC method was carried out as per the ICH requirements. The developed method was found to be suitable to check the quality of bulk samples of Ambrisentan at the time of batch release and also during its storage (long-term and accelerated stability). © The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Measurement of the specific surface area of loose copper deposit by electrochemical methods

    Directory of Open Access Journals (Sweden)

    E. A. Dolmatova

    2016-07-01

    Full Text Available In the work the surface area of the electrode with dispersed copper deposit obtained within 30 seconds was evaluated by techniques of chronopotentiometry (CPM and impedance spectroscopy. In method CPM the electrode surface available for measurement depends on the value of the polarizing current. At high currents during the transition time there is a change of surface relief that can not determine the full surface of loose deposit. The electrochemical impedance method is devoid of this shortcoming since the measurements are carried out in indifferent electrolyte in the absence of current. The area measured by the impedance is tens of times higher than the value obtained by chronopotentiometry. It is found that from a solution containing sulfuric acid the deposits form with a high specific surface area. Based on these data it was concluded that the method of impedance spectroscopy can be used to measure in situ the surface area of the dispersed copper deposits.

  3. HadISDH: an updateable land surface specific humidity product for climate monitoring

    Directory of Open Access Journals (Sweden)

    K. M. Willett

    2013-03-01

    Full Text Available HadISDH is a near-global land surface specific humidity monitoring product providing monthly means from 1973 onwards over large-scale grids. Presented herein to 2012, annual updates are anticipated. HadISDH is an update to the land component of HadCRUH, utilising the global high-resolution land surface station product HadISD as a basis. HadISD, in turn, uses an updated version of NOAA's Integrated Surface Database. Intensive automated quality control has been undertaken at the individual observation level, as part of HadISD processing. The data have been subsequently run through the pairwise homogenisation algorithm developed for NCDC's US Historical Climatology Network monthly temperature product. For the first time, uncertainty estimates are provided at the grid-box spatial scale and monthly timescale. HadISDH is in good agreement with existing land surface humidity products in periods of overlap, and with both land air and sea surface temperature estimates. Widespread moistening is shown over the 1973–2012 period. The largest moistening signals are over the tropics with drying over the subtropics, supporting other evidence of an intensified hydrological cycle over recent years. Moistening is detectable with high (95% confidence over large-scale averages for the globe, Northern Hemisphere and tropics, with trends of 0.089 (0.080 to 0.098 g kg−1 per decade, 0.086 (0.075 to 0.097 g kg−1 per decade and 0.133 (0.119 to 0.148 g kg−1 per decade, respectively. These changes are outside the uncertainty range for the large-scale average which is dominated by the spatial coverage component; station and grid-box sampling uncertainty is essentially negligible on large scales. A very small moistening (0.013 (−0.005 to 0.031 g kg−1 per decade is found in the Southern Hemisphere, but it is not significantly different from zero and uncertainty is large. When globally averaged, 1998 is the moistest year since monitoring began in 1973, closely

  4. A systematic design method for robust synthetic biology to satisfy design specifications.

    Science.gov (United States)

    Chen, Bor-Sen; Wu, Chih-Hung

    2009-06-30

    Synthetic biology is foreseen to have important applications in biotechnology and medicine, and is expected to contribute significantly to a better understanding of the functioning of complex biological systems. However, the development of synthetic gene networks is still difficult and most newly created gene networks are non-functioning due to intrinsic parameter uncertainties, external disturbances and functional variations of intra- and extra-cellular environments. The design method for a robust synthetic gene network that works properly in a host cell under these intrinsic parameter uncertainties and external disturbances is the most important topic in synthetic biology. In this study, we propose a stochastic model that includes parameter fluctuations and external disturbances to mimic the dynamic behaviors of a synthetic gene network in the host cell. Then, based on this stochastic model, four design specifications are introduced to guarantee that a synthetic gene network can achieve its desired steady state behavior in spite of parameter fluctuations, external disturbances and functional variations in the host cell. We propose a systematic method to select a set of appropriate design parameters for a synthetic gene network that will satisfy these design specifications so that the intrinsic parameter fluctuations can be tolerated, the external disturbances can be efficiently filtered, and most importantly, the desired steady states can be achieved. Thus the synthetic gene network can work properly in a host cell under intrinsic parameter uncertainties, external disturbances and functional variations. Finally, a design procedure for the robust synthetic gene network is developed and a design example is given in silico to confirm the performance of the proposed method. Based on four design specifications, a systematic design procedure is developed for designers to engineer a robust synthetic biology network that can achieve its desired steady state behavior

  5. Methods and measures of enhancing production capacity of uranium mines

    International Nuclear Information System (INIS)

    Ni Yuhui

    2013-01-01

    Limited by resource conditions and mining conditions, the production capacity of uranium mines is generally small. The main factors to affect the production capacity determination of uranium mines are analyzed, the ways and measures to enhance the production capacity of uranium mines are explored from the innovations of technology and management mode. (author)

  6. PRODUCTION PROGRAM OPTIMIZATION – RESPONSIBLE MANAGEMENT METHOD OF LOGGING

    Directory of Open Access Journals (Sweden)

    Severian Vlăduț IACOB

    2014-06-01

    Full Text Available Aggression of any kind on the environment and especially uncontrolled exploitation of forests has generated mixed reaction among the public and politicians, but especially in the academic sphere where actually were fired early warning signs of the effects of these actions. Reduction of Earth's forested areas was discussed by specialists as continuous and highly accelerated, especially in the last century when reserve "green gold" of mankind has halved. As a result, the conservation of forests and prevention of environmental degradation have become great concerns of each nation, but the need for global action required the transformation into strategic objectives of regional and international bodies. They have designed policies and measures generally available and have created favorable framework implementation in the Member States and beyond. One of the projects that came to life and expanded worldwide was the sustainable development today, basically there are no area where the activity is not subrogated to achieve a balance between the components of economic, social and environmental. From this perspective forestry and forestry exploitation is the place where really have achieved sustainability. Achieving this requirement becomes an obligation, both for woodland owners and operators seeking to obtain profit from logging. Compliance and ensuring responsible management are certainly prerequisites of sustainability in this sector. Scientific planning of production is a method of management responsible. This study takes into account, on the one hand, highlighting the effects generated by uncontrolled deforestation and the importance of sustainable forest exploitation, and on the other hand, presenting the need to use scientific methods to practice responsible management.

  7. A Simplified Method for Laboratory Preparation of Organ Specific Indium 113m Compounds

    Energy Technology Data Exchange (ETDEWEB)

    Adatepe, M H; Potchen, E James [Washington University School of Medicine, St. Louis (United States)

    1969-03-15

    Generator systems producing short lived nuclides from longer lived parents have distinct clinical advantages. They are more economical, result in a lower radiation dose, and can make short lived scanning readily available even in areas remote from rapid radiopharmaceutical delivery services. The {sup 113}Sn-{sup 113m}In generator has the additional advantage that, as a transition metal, Indium can be readily complexed into organ specific preparations. 113Sn, a reactor produced nuclide with a 118 day half life, is absorbed on a zirconium or silica gel column. the generator is eluded with 5 to 8 ml of 0.05 N HCL solution at pH 1.3-1.4. The daughter nuclide, {sup 113m}In, has a half life of 1.7 hours and emits a 393 Kev monoenergetic gamma ray. Previous methods for labeling organ specific complexes with {sup 113m}In required terminal autoclaving before injection. With the recent introduction of sterile, apyrogenic {sup 113}Sn-{sup 113m}In generators, we have developed a simplified technique for the laboratory preparation of Indium labeled compounds. This method eliminates autoclaving and titration enabling us to pre-prepare organ specific complexes for blood pool, liver, spleen, brain, kidney and lung scanning.

  8. Perspectives and strategies of alternative methods used in the risk assessment of personal care products.

    Science.gov (United States)

    Quantin, P; Thélu, A; Catoire, S; Ficheux, H

    2015-11-01

    Risk assessment for personal care products requires the use of alternative methods since animal testing is now totally banned. Some of these methods are effective and have been validated by the "European Union Reference Laboratory for alternatives to animal testing"; but there is still a need for development and implementation of methods for specific endpoints. In this review, we have focused on dermal risk assessment because it is the prime route of absorption and main target organ for personal care products. Within this field, various areas must be assessed: irritation, sensitisation and toxicokinetic. Personal care product behaviour after use by the consumer and potential effects on the environment are also discussed. The purpose of this review is to show evolution and the prospects of alternative methods for safety dermal assessment. Assessment strategies must be adapted to the different chemical classes of substances studied but also to the way in which they are used. Finally, experimental and theoretical technical parameters that may impact on measured effects have been identified and discussed. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  9. Simple and high yielding method for preparing tissue specific extracellular matrix coatings for cell culture.

    Science.gov (United States)

    DeQuach, Jessica A; Mezzano, Valeria; Miglani, Amar; Lange, Stephan; Keller, Gordon M; Sheikh, Farah; Christman, Karen L

    2010-09-27

    The native extracellular matrix (ECM) consists of a highly complex, tissue-specific network of proteins and polysaccharides, which help regulate many cellular functions. Despite the complex nature of the ECM, in vitro cell-based studies traditionally assess cell behavior on single ECM component substrates, which do not adequately mimic the in vivo extracellular milieu. We present a simple approach for developing naturally derived ECM coatings for cell culture that provide important tissue-specific cues unlike traditional cell culture coatings, thereby enabling the maturation of committed C2C12 skeletal myoblast progenitors and human embryonic stem cells differentiated into cardiomyocytes. Here we show that natural muscle-specific coatings can (i) be derived from decellularized, solubilized adult porcine muscle, (ii) contain a complex mixture of ECM components including polysaccharides, (iii) adsorb onto tissue culture plastic and (iv) promote cell maturation of committed muscle progenitor and stem cells. This versatile method can create tissue-specific ECM coatings, which offer a promising platform for cell culture to more closely mimic the mature in vivo ECM microenvironment.

  10. Engineering Bacteria to Search for Specific Concentrations of Molecules by a Systematic Synthetic Biology Design Method.

    Science.gov (United States)

    Tien, Shin-Ming; Hsu, Chih-Yuan; Chen, Bor-Sen

    2016-01-01

    Bacteria navigate environments full of various chemicals to seek favorable places for survival by controlling the flagella's rotation using a complicated signal transduction pathway. By influencing the pathway, bacteria can be engineered to search for specific molecules, which has great potential for application to biomedicine and bioremediation. In this study, genetic circuits were constructed to make bacteria search for a specific molecule at particular concentrations in their environment through a synthetic biology method. In addition, by replacing the "brake component" in the synthetic circuit with some specific sensitivities, the bacteria can be engineered to locate areas containing specific concentrations of the molecule. Measured by the swarm assay qualitatively and microfluidic techniques quantitatively, the characteristics of each "brake component" were identified and represented by a mathematical model. Furthermore, we established another mathematical model to anticipate the characteristics of the "brake component". Based on this model, an abundant component library can be established to provide adequate component selection for different searching conditions without identifying all components individually. Finally, a systematic design procedure was proposed. Following this systematic procedure, one can design a genetic circuit for bacteria to rapidly search for and locate different concentrations of particular molecules by selecting the most adequate "brake component" in the library. Moreover, following simple procedures, one can also establish an exclusive component library suitable for other cultivated environments, promoter systems, or bacterial strains.

  11. Engineering Bacteria to Search for Specific Concentrations of Molecules by a Systematic Synthetic Biology Design Method.

    Directory of Open Access Journals (Sweden)

    Shin-Ming Tien

    Full Text Available Bacteria navigate environments full of various chemicals to seek favorable places for survival by controlling the flagella's rotation using a complicated signal transduction pathway. By influencing the pathway, bacteria can be engineered to search for specific molecules, which has great potential for application to biomedicine and bioremediation. In this study, genetic circuits were constructed to make bacteria search for a specific molecule at particular concentrations in their environment through a synthetic biology method. In addition, by replacing the "brake component" in the synthetic circuit with some specific sensitivities, the bacteria can be engineered to locate areas containing specific concentrations of the molecule. Measured by the swarm assay qualitatively and microfluidic techniques quantitatively, the characteristics of each "brake component" were identified and represented by a mathematical model. Furthermore, we established another mathematical model to anticipate the characteristics of the "brake component". Based on this model, an abundant component library can be established to provide adequate component selection for different searching conditions without identifying all components individually. Finally, a systematic design procedure was proposed. Following this systematic procedure, one can design a genetic circuit for bacteria to rapidly search for and locate different concentrations of particular molecules by selecting the most adequate "brake component" in the library. Moreover, following simple procedures, one can also establish an exclusive component library suitable for other cultivated environments, promoter systems, or bacterial strains.

  12. PREDICTION OF MEAT PRODUCT QUALITY BY THE MATHEMATICAL PROGRAMMING METHODS

    Directory of Open Access Journals (Sweden)

    A. B. Lisitsyn

    2016-01-01

    Full Text Available Abstract Use of the prediction technologies is one of the directions of the research work carried out both in Russia and abroad. Meat processing is accompanied by the complex physico-chemical, biochemical and mechanical processes. To predict the behavior of meat raw material during the technological processing, a complex of physico-technological and structural-mechanical indicators, which objectively reflects its quality, is used. Among these indicators are pH value, water binding and fat holding capacities, water activity, adhesiveness, viscosity, plasticity and so on. The paper demonstrates the influence of animal proteins (beef and pork on the physico-chemical and functional properties before and after thermal treatment of minced meat made from meat raw material with different content of the connective and fat tissues. On the basis of the experimental data, the model (stochastic dependence parameters linking the quantitative resultant and factor variables were obtained using the regression analysis, and the degree of the correlation with the experimental data was assessed. The maximum allowable levels of meat raw material replacement with animal proteins (beef and pork were established by the methods of mathematical programming. Use of the information technologies will significantly reduce the costs of the experimental search and substantiation of the optimal level of replacement of meat raw material with animal proteins (beef, pork, and will also allow establishing a relationship of product quality indicators with quantity and quality of minced meat ingredients.

  13. Effectiveness of methods for reducing acrylamide in bakery products.

    Science.gov (United States)

    Sadd, Peter A; Hamlet, Colin G; Liang, Li

    2008-08-13

    Pilot-scale bread, biscuit, and cracker doughs have been baked to assess how well recipe changes could reduce acrylamide in commercial bakery products. Removing ammonium-based raising agents was beneficial in biscuits. In doughs, long yeast fermentations were an effective way of reducing asparagine levels and hence acrylamide. At moderate fermentation times fructose levels increased, but the yeast later absorbed this, so the net effect on acrylamide was beneficial. Metal ions such as calcium reduced acrylamide when added as the carbonate or chloride. Hence, the fortification of flour with calcium carbonate, over and above its natural mineral content, has an additional benefit. However, some other possible methods of adding calcium to bakery doughs, for example, via the permitted preservative calcium propionate, were not beneficial. Amino acid addition to doughs gave modest reductions in acrylamide. Lowering the dough pH reduced acrylamide, but at the expense of higher levels of other process contaminants such as 3-monochloropropane-1,2-diol (3-MCPD).

  14. User input in iterative design for prevention product development: leveraging interdisciplinary methods to optimize effectiveness.

    Science.gov (United States)

    Guthrie, Kate M; Rosen, Rochelle K; Vargas, Sara E; Guillen, Melissa; Steger, Arielle L; Getz, Melissa L; Smith, Kelley A; Ramirez, Jaime J; Kojic, Erna M

    2017-10-01

    The development of HIV-preventive topical vaginal microbicides has been challenged by a lack of sufficient adherence in later stage clinical trials to confidently evaluate effectiveness. This dilemma has highlighted the need to integrate translational research earlier in the drug development process, essentially applying behavioral science to facilitate the advances of basic science with respect to the uptake and use of biomedical prevention technologies. In the last several years, there has been an increasing recognition that the user experience, specifically the sensory experience, as well as the role of meaning-making elicited by those sensations, may play a more substantive role than previously thought. Importantly, the role of the user-their sensory perceptions, their judgements of those experiences, and their willingness to use a product-is critical in product uptake and consistent use post-marketing, ultimately realizing gains in global public health. Specifically, a successful prevention product requires an efficacious drug, an efficient drug delivery system, and an effective user. We present an integrated iterative drug development and user experience evaluation method to illustrate how user-centered formulation design can be iterated from the early stages of preclinical development to leverage the user experience. Integrating the user and their product experiences into the formulation design process may help optimize both the efficiency of drug delivery and the effectiveness of the user.

  15. The methodical substantiation of measures to improve the use of basic production assets of the enterprise

    Directory of Open Access Journals (Sweden)

    Korol Svetlana Anatolevna

    2013-07-01

    Full Text Available The method of calculating the performance measures to improve the use of basic production assets of the enterprise: production cost, production volume, number of additional workers, depreciation and amortization.

  16. Production, characterization and applications for Toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins.

    Directory of Open Access Journals (Sweden)

    Álvaro Ferreira Júnior

    Full Text Available Toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. Immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. Unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interference with rheumatoid factor, complement activation and affinity to Fc mammalian receptors. IgY antibodies avoid those limitations; therefore they could be an alternative to be applied in T. gondii model.In this study we immunized hens with soluble tachyzoite antigens of T. gondii (STAg and purified egg yolk antibodies (IgY by an inexpensive and simple method, with high yield and purity degree. IgY anti-STAg antibodies presented high avidity and were able to recognize a broad range of parasite antigens, although some marked differences were observed in reactivity profile between antibodies produced in immunized hens and mice. Interestingly, IgY antibodies against Neospora caninum and Eimeria spp. did not react to STAg. We also show that IgY antibodies were suitable to detect T. gondii forms in paraffin-embedded sections and culture cell monolayers.Due to its cost-effectiveness, high production yield and varied range of possible applications, polyclonal IgY antibodies are useful tools for studies involving T. gondii.

  17. Study of the 99Mo production effect on some of research reactor core specification using DARE-P

    International Nuclear Information System (INIS)

    Khamis, I.; Ezzuddin, H.

    2006-12-01

    In this study, the major basis of Mo- production using irradiation method for targets of enriched uranium is presented. Factors such as neutron flux, time of irradiation, and enrichment ration have also been analyzed. It was concluded that Mo-production requires nuclear reactors having high neutron fluxes, and that raising the neutron flux results in increasing the Mo-activities especially at fluxes that are higher than 10 14 n.cm -2 .s -1 . Time of irradiation was found to have adverse i.e. positive and negative effects on Mo activity. Therefore, the optimum irradiation time should be determined. The Mo-production was modeled through a set of differential equation with respect to time with aim to study the most influencing factors on Mo production, especially the effects of neutron flux and time of irradiation. In addition, the mass of plutonium produced during the irradiation of low enriched uranium has been evaluated. An investigation as to whether 99 Mo could be produced in the Syrian MNSR has been made. The result shows that a specific activity of 0.3773 Ci/g for 99 Mo could be produced. In future, a nuclear research reactor having neutron flux of 10 14 n.cm -2 .s -1 and 8 irradiation sites suitable for targets of 5 g of 235 U could produce about 5000 Ci on a weekly. Calculation and simulation have been achieved using the already developed DARE-P simulation language which was modified and improved in the nuclear Engineering Department in the Atomic Energy Commission of Syria. (author)

  18. Specific algorithm method of scoring the Clock Drawing Test applied in cognitively normal elderly

    Directory of Open Access Journals (Sweden)

    Liana Chaves Mendes-Santos

    Full Text Available The Clock Drawing Test (CDT is an inexpensive, fast and easily administered measure of cognitive function, especially in the elderly. This instrument is a popular clinical tool widely used in screening for cognitive disorders and dementia. The CDT can be applied in different ways and scoring procedures also vary. OBJECTIVE: The aims of this study were to analyze the performance of elderly on the CDT and evaluate inter-rater reliability of the CDT scored by using a specific algorithm method adapted from Sunderland et al. (1989. METHODS: We analyzed the CDT of 100 cognitively normal elderly aged 60 years or older. The CDT ("free-drawn" and Mini-Mental State Examination (MMSE were administered to all participants. Six independent examiners scored the CDT of 30 participants to evaluate inter-rater reliability. RESULTS AND CONCLUSION: A score of 5 on the proposed algorithm ("Numbers in reverse order or concentrated", equivalent to 5 points on the original Sunderland scale, was the most frequent (53.5%. The CDT specific algorithm method used had high inter-rater reliability (p<0.01, and mean score ranged from 5.06 to 5.96. The high frequency of an overall score of 5 points may suggest the need to create more nuanced evaluation criteria, which are sensitive to differences in levels of impairment in visuoconstructive and executive abilities during aging.

  19. Temporal Changes in Coupled Vegetation Phenology and Productivity are Biome-Specific in the Northern Hemisphere

    Directory of Open Access Journals (Sweden)

    Lanhui Wang

    2017-12-01

    Full Text Available Global warming has greatly stimulated vegetation growth through both extending the growing season and promoting photosynthesis in the Northern Hemisphere (NH. Analyzing the combined dynamics of such trends can potentially improve our current understanding on changes in vegetation functioning and the complex relationship between anthropogenic and climatic drivers. This study aims to analyze the relationships (long-term trends and correlations of length of vegetation growing season (LOS and vegetation productivity assessed by the growing season NDVI integral (GSI in the NH (>30°N to study any dependency of major biomes that are characterized by different imprint from anthropogenic influence. Spatial patterns of converging/diverging trends in LOS and GSI and temporal changes in the coupling between LOS and GSI are analyzed for major biomes at hemispheric and continental scales from the third generation Global Inventory Monitoring and Modeling Studies (GIMMS Normalized Difference Vegetation Index (NDVI dataset for a 32-year period (1982–2013. A quarter area of the NH is covered by converging trends (consistent significant trends in LOS and GSI, whereas diverging trends (opposing significant trends in LOS and GSI cover about 6% of the region. Diverging trends are observed mainly in high latitudes and arid/semi-arid areas of non-forest biomes (shrublands, savannas, and grasslands, whereas forest biomes and croplands are primarily characterized by converging trends. The study shows spatially-distinct and biome-specific patterns between the continental land masses of Eurasia (EA and North America (NA. Finally, areas of high positive correlation between LOS and GSI showed to increase during the period of analysis, with areas of significant positive trends in correlation being more widespread in NA as compared to EA. The temporal changes in the coupled vegetation phenology and productivity suggest complex relationships and interactions that are induced

  20. Managing Variety in Configure-to-Order Products - An Operational Method

    DEFF Research Database (Denmark)

    Myrodia, Anna; Hvam, Lars

    2014-01-01

    is to develop an operational method to analyze profitability of Configure-To-Order (CTO) products. The operational method consists of a four-step: analysis of product assortment, profitability analysis on configured products, market and competitor analysis and, product assortment scenarios analysis....... The proposed operational method is firstly developed based on both available literature and practitioners experience and subsequently tested on a company that produces CTO products. The results from this application are further discussed and opportunities for further research identified....

  1. Novel Biocontrol Methods for Listeria monocytogenes Biofilms in Food Production Facilities

    Directory of Open Access Journals (Sweden)

    Jessica A. Gray

    2018-04-01

    Full Text Available High mortality and hospitalization rates have seen Listeria monocytogenes as a foodborne pathogen of public health importance for many years and of particular concern for high-risk population groups. Food manufactures face an ongoing challenge in preventing the entry of L. monocytogenes into food production environments (FPEs due to its ubiquitous nature. In addition to this, the capacity of L. monocytogenes strains to colonize FPEs can lead to repeated identification of L. monocytogenes in FPE surveillance. The contamination of food products requiring product recall presents large economic burden to industry and is further exacerbated by damage to the brand. Poor equipment design, facility layout, and worn or damaged equipment can result in Listeria hotspots and biofilms where traditional cleaning and disinfecting procedures may be inadequate. Novel biocontrol methods may offer FPEs effective means to help improve control of L. monocytogenes and decrease cross contamination of food. Bacteriophages have been used as a medical treatment for many years for their ability to infect and lyse specific bacteria. Endolysins, the hydrolytic enzymes of bacteriophages responsible for breaking the cell wall of Gram-positive bacteria, are being explored as a biocontrol method for food preservation and in nanotechnology and medical applications. Antibacterial proteins known as bacteriocins have been used as alternatives to antibiotics for biopreservation and food product shelf life extension. Essential oils are natural antimicrobials formed by plants and have been used as food additives and preservatives for many years and more recently as a method to prevent food spoilage by microorganisms. Competitive exclusion occurs naturally among bacteria in the environment. However, intentionally selecting and applying bacteria to effect competitive exclusion of food borne pathogens has potential as a biocontrol application. This review discusses these novel biocontrol

  2. Novel Biocontrol Methods for Listeria monocytogenes Biofilms in Food Production Facilities.

    Science.gov (United States)

    Gray, Jessica A; Chandry, P Scott; Kaur, Mandeep; Kocharunchitt, Chawalit; Bowman, John P; Fox, Edward M

    2018-01-01

    High mortality and hospitalization rates have seen Listeria monocytogenes as a foodborne pathogen of public health importance for many years and of particular concern for high-risk population groups. Food manufactures face an ongoing challenge in preventing the entry of L. monocytogenes into food production environments (FPEs) due to its ubiquitous nature. In addition to this, the capacity of L. monocytogenes strains to colonize FPEs can lead to repeated identification of L. monocytogenes in FPE surveillance. The contamination of food products requiring product recall presents large economic burden to industry and is further exacerbated by damage to the brand. Poor equipment design, facility layout, and worn or damaged equipment can result in Listeria hotspots and biofilms where traditional cleaning and disinfecting procedures may be inadequate. Novel biocontrol methods may offer FPEs effective means to help improve control of L. monocytogenes and decrease cross contamination of food. Bacteriophages have been used as a medical treatment for many years for their ability to infect and lyse specific bacteria. Endolysins, the hydrolytic enzymes of bacteriophages responsible for breaking the cell wall of Gram-positive bacteria, are being explored as a biocontrol method for food preservation and in nanotechnology and medical applications. Antibacterial proteins known as bacteriocins have been used as alternatives to antibiotics for biopreservation and food product shelf life extension. Essential oils are natural antimicrobials formed by plants and have been used as food additives and preservatives for many years and more recently as a method to prevent food spoilage by microorganisms. Competitive exclusion occurs naturally among bacteria in the environment. However, intentionally selecting and applying bacteria to effect competitive exclusion of food borne pathogens has potential as a biocontrol application. This review discusses these novel biocontrol methods and their

  3. Novel Biocontrol Methods for Listeria monocytogenes Biofilms in Food Production Facilities

    Science.gov (United States)

    Gray, Jessica A.; Chandry, P. Scott; Kaur, Mandeep; Kocharunchitt, Chawalit; Bowman, John P.; Fox, Edward M.

    2018-01-01

    High mortality and hospitalization rates have seen Listeria monocytogenes as a foodborne pathogen of public health importance for many years and of particular concern for high-risk population groups. Food manufactures face an ongoing challenge in preventing the entry of L. monocytogenes into food production environments (FPEs) due to its ubiquitous nature. In addition to this, the capacity of L. monocytogenes strains to colonize FPEs can lead to repeated identification of L. monocytogenes in FPE surveillance. The contamination of food products requiring product recall presents large economic burden to industry and is further exacerbated by damage to the brand. Poor equipment design, facility layout, and worn or damaged equipment can result in Listeria hotspots and biofilms where traditional cleaning and disinfecting procedures may be inadequate. Novel biocontrol methods may offer FPEs effective means to help improve control of L. monocytogenes and decrease cross contamination of food. Bacteriophages have been used as a medical treatment for many years for their ability to infect and lyse specific bacteria. Endolysins, the hydrolytic enzymes of bacteriophages responsible for breaking the cell wall of Gram-positive bacteria, are being explored as a biocontrol method for food preservation and in nanotechnology and medical applications. Antibacterial proteins known as bacteriocins have been used as alternatives to antibiotics for biopreservation and food product shelf life extension. Essential oils are natural antimicrobials formed by plants and have been used as food additives and preservatives for many years and more recently as a method to prevent food spoilage by microorganisms. Competitive exclusion occurs naturally among bacteria in the environment. However, intentionally selecting and applying bacteria to effect competitive exclusion of food borne pathogens has potential as a biocontrol application. This review discusses these novel biocontrol methods and their

  4. Specific and Class Object Recognition for Service Robots through Autonomous and Interactive Methods

    Science.gov (United States)

    Mansur, Al; Kuno, Yoshinori

    Service robots need to be able to recognize and identify objects located within complex backgrounds. Since no single method may work in every situation, several methods need to be combined and robots have to select the appropriate one automatically. In this paper we propose a scheme to classify situations depending on the characteristics of the object of interest and user demand. We classify situations into four groups and employ different techniques for each. We use Scale-invariant feature transform (SIFT), Kernel Principal Components Analysis (KPCA) in conjunction with Support Vector Machine (SVM) using intensity, color, and Gabor features for five object categories. We show that the use of appropriate features is important for the use of KPCA and SVM based techniques on different kinds of objects. Through experiments we show that by using our categorization scheme a service robot can select an appropriate feature and method, and considerably improve its recognition performance. Yet, recognition is not perfect. Thus, we propose to combine the autonomous method with an interactive method that allows the robot to recognize the user request for a specific object and class when the robot fails to recognize the object. We also propose an interactive way to update the object model that is used to recognize an object upon failure in conjunction with the user's feedback.

  5. A novel analysis strategy for HLA typing using a sequence-specific oligonucleotide probe method.

    Science.gov (United States)

    Won, D I

    2017-11-01

    The technique of reverse sequence-specific oligonucleotide probes (SSOPs) is commonly used in human leukocyte antigen (HLA) typing. In the conventional method for data analysis (exact pattern matching, EPM), the larger is the number of mismatched probes, the longer the time for final typing assignment. A novel strategy, filtering and scoring (FnS), has been developed to easily assign the best-fit allele pair. In the FnS method, candidate alleles and allele pairs were filtered based on (1) subject's ethnicity, and (2) the measured partial reaction pattern with only definitely negative or positive probes. Then, the complete reaction pattern for all probes (CRPoAPs) were compared between the raw sample and expected residual allele pairs to obtain mismatch scores. To compare the FnS and EPM methods, each analysis time (minutes:seconds) for reverse SSOP HLA typing with intermediate resolution (n = 507) was measured. The analysis time with FnS method was shorter than that of the EPM method [00:21 (00:08-01:47) and 01:04 (00:15-23:45), respectively, P typing in a comprehensive and quantitative comparison between measured and expected CRPoAPs of candidate allele pairs. Therefore, this analysis strategy might be useful in a clinical setting. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  6. Physiological level production of antigen-specific human immunoglobulin in cloned transchromosomic cattle.

    Directory of Open Access Journals (Sweden)

    Akiko Sano

    Full Text Available Therapeutic human polyclonal antibodies (hpAbs derived from pooled plasma from human donors are Food and Drug Administration approved biologics used in the treatment of a variety of human diseases. Powered by the natural diversity of immune response, hpAbs are effective in treating diseases caused by complex or quickly-evolving antigens such as viruses. We previously showed that transchromosomic (Tc cattle carrying a human artificial chromosome (HAC comprising the entire unrearranged human immunoglobulin heavy-chain (hIGH and kappa-chain (hIGK germline loci (named as κHAC are capable of producing functional hpAbs when both of the bovine immunoglobulin mu heavy-chains, bIGHM and bIGHML1, are homozygously inactivated (double knockouts or DKO. However, B lymphocyte development in these Tc cattle is compromised, and the overall production of hpAbs is low. Here, we report the construction of an improved HAC, designated as cKSL-HACΔ, by incorporating all of the human immunoglobulin germline loci into the HAC. Furthermore, for avoiding the possible human-bovine interspecies incompatibility between the human immunoglobulin mu chain protein (hIgM and bovine transmembrane α and β immunoglobulins (bIgα and bIgβ in the pre-B cell receptor (pre-BCR complex, we partially replaced (bovinized the hIgM constant domain with the counterpart of bovine IgM (bIgM that is involved in the interaction between bIgM and bIgα/Igβ; human IgM bovinization would also improve the functionality of hIgM in supporting B cell activation and proliferation. We also report the successful production of DKO Tc cattle carrying the cKSL-HACΔ (cKSL-HACΔ/DKO, the dramatic improvement of B cell development in these cattle and the high level production of hpAbs (as measured for the human IgG isotype in the plasma. We further demonstrate that, upon immunization by tumor immunogens, high titer tumor immunogen-specific human IgG (hIgG can be produced from such Tc cattle.

  7. Application of modeling methods for an estimation of a specific activity 137Cs geologic environment

    International Nuclear Information System (INIS)

    Kalinovskij, A.K.; Batij, V.G.; Pravdivyj, A.A.; Krasnov, V.A.

    2004-01-01

    The manual application of methods of mathematical and physical modeling for an estimation of the specific activity 137 Cs in soils composing a geological profile of site of object 'Ukryttya' is demonstrated. The calculations are executed by the software packages of Micro Shield, CYCLON, MCNP5. The experimental measurements are carried out by logging radiometers of different type on the borehole models. The value of a conversion coefficient of infinite environment for quantitative interpretation of gamma-ray logging data is determined over calculations outcomes and experimental measurements. The calculated and experimental values have agreement among themselves. the error estimation of the obtained outcomes is executed. 26 refs., 3 tab., 10 figs

  8. SPECIFIC MANAGEMENT AND LEADING METHODS APPLIED TO BUTTERFLY TOURISM FOR 2012

    Directory of Open Access Journals (Sweden)

    Eugenia ALECU

    2013-12-01

    Full Text Available In this paper are explained the most important management elements for managers and employees of a tour operator agency. The study showed in a concrete and realistic manner how a manager of a tour operator agency can lead, control, predict and determine the prospects utilizing a specific management method called dashboard. Given the competition in the field of tourism, Butterfly Tourism is trying to manage its business in a professional manner and offer attractive packages and favorable prices to its customers.

  9. Crop and soil specific N and P efficiency and productivity in Finland

    NARCIS (Netherlands)

    Bäckman, S.; Oude Lansink, A.G.J.M.

    2005-01-01

    This paper estimates a stochastic production frontier based on experimental data of cereals production in Finland over the period 1977-1994. The estimates of the production frontier are used to analyze nitrogen and phosphorous productivity and efficiency differences between soils and crops. For this

  10. Comparison of methods to identify crop productivity constraints in developing countries. A review

    NARCIS (Netherlands)

    Kraaijvanger, R.G.M.; Sonneveld, M.P.W.; Almekinders, C.J.M.; Veldkamp, T.

    2015-01-01

    Selecting a method for identifying actual crop productivity constraints is an important step for triggering innovation processes. Applied methods can be diverse and although such methods have consequences for the design of intervention strategies, documented comparisons between various methods are

  11. Decontamination method and device for radiation contaminated product

    International Nuclear Information System (INIS)

    Morikawa, Kenji; Ohinata, Hiroshi; Omata, Kazuo; Sato, Toshihiko; Nakajima, Yoshihiko; Ichikawa, Seigo.

    1996-01-01

    In the present invention, radiation contaminated products generated during shot peening are decontaminated by a chelating agent, and the chelating agent is removed from the radiation contaminated products. Then the temperature of the radiation contaminated products is elevated by hot blowing at a temperature higher than a boiling point of the solvent. Then, a solvent is added to the radiation contaminated products and the solvent is evaporated abruptly. The solution of the chelating agent remained while being deposited thereto is removed by evaporation to remove it from the radiation contaminated products together with the solvent. With such procedures, all of the decontamination steps can be completed in one device without requiring a large space or not moving the radiation contaminated products on every step. (T.M.)

  12. Rotator cuff tear reduces muscle fiber specific force production and induces macrophage accumulation and autophagy.

    Science.gov (United States)

    Gumucio, Jonathan P; Davis, Max E; Bradley, Joshua R; Stafford, Patrick L; Schiffman, Corey J; Lynch, Evan B; Claflin, Dennis R; Bedi, Asheesh; Mendias, Christopher L

    2012-12-01

    Full-thickness tears to the rotator cuff can cause severe pain and disability. Untreated tears progress in size and are associated with muscle atrophy and an infiltration of fat to the area, a condition known as "fatty degeneration." To improve the treatment of rotator cuff tears, a greater understanding of the changes in the contractile properties of muscle fibers and the molecular regulation of fatty degeneration is essential. Using a rat model of rotator cuff injury, we measured the force generating capacity of individual muscle fibers and determined changes in muscle fiber type distribution that develop after a full thickness rotator cuff tear. We also measured the expression of mRNA and miRNA transcripts involved in muscle atrophy, lipid accumulation, and matrix synthesis. We hypothesized that a decrease in specific force of rotator cuff muscle fibers, an accumulation of type IIb fibers, and an upregulation in fibrogenic, adipogenic, and inflammatory gene expression occur in torn rotator cuff muscles. Thirty days following rotator cuff tear, we observed a reduction in muscle fiber force production, an induction of fibrogenic, adipogenic, and autophagocytic mRNA and miRNA molecules, and a dramatic accumulation of macrophages in areas of fat accumulation. Copyright © 2012 Orthopaedic Research Society.

  13. First applications of structural pattern recognition methods to the investigation of specific physical phenomena at JET

    International Nuclear Information System (INIS)

    Ratta, G.A.; Vega, J.; Pereira, A.; Portas, A.; Luna, E. de la; Dormido-Canto, S.; Farias, G.; Dormido, R.; Sanchez, J.; Duro, N.; Vargas, H.; Santos, M.; Pajares, G.; Murari, A.

    2008-01-01

    Structural pattern recognition techniques allow the identification of plasma behaviours. Physical properties are encoded in the morphological structure of signals. Intelligent access methods have been applied to JET databases to retrieve data according to physical criteria. On the one hand, the structural form of signals has been used to develop general purpose data retrieval systems to search for both similar entire waveforms and similar structural shapes inside waveforms. On the other hand, domain dependent knowledge was added to the structural information of signals to create particular data retrieval methods for specific physical phenomena. The inclusion of explicit knowledge assists in data analysis. The latter has been applied in JET to look for first, cut-offs in ECE heterodyne radiometer signals and, second, L-H transitions

  14. First applications of structural pattern recognition methods to the investigation of specific physical phenomena at JET

    Energy Technology Data Exchange (ETDEWEB)

    Ratta, G.A. [Asociacion EURATOM/CIEMAT para Fusion (Spain)], E-mail: giuseppe.ratta@ciemat.es; Vega, J.; Pereira, A.; Portas, A.; Luna, E. de la [Asociacion EURATOM/CIEMAT para Fusion (Spain); Dormido-Canto, S.; Farias, G.; Dormido, R.; Sanchez, J.; Duro, N.; Vargas, H. [Dpto. Informatica y Automatica-UNED, 28040 Madrid (Spain); Santos, M.; Pajares, G. [Dpto. Arquitectura de Computadores y Automatica-UCM, 28040 Madrid (Spain); Murari, A. [Consorzio RFX-Associazione EURATOM ENEA per la Fusione, Padua (Italy)

    2008-04-15

    Structural pattern recognition techniques allow the identification of plasma behaviours. Physical properties are encoded in the morphological structure of signals. Intelligent access methods have been applied to JET databases to retrieve data according to physical criteria. On the one hand, the structural form of signals has been used to develop general purpose data retrieval systems to search for both similar entire waveforms and similar structural shapes inside waveforms. On the other hand, domain dependent knowledge was added to the structural information of signals to create particular data retrieval methods for specific physical phenomena. The inclusion of explicit knowledge assists in data analysis. The latter has been applied in JET to look for first, cut-offs in ECE heterodyne radiometer signals and, second, L-H transitions.

  15. Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method

    International Nuclear Information System (INIS)

    Lu, Heng; Wen, Juan; Wang, Xu; Yuan, Kun; Lu, Huibin; Zhou, Yueliang; Jin, Kuijuan; Yang, Guozhen; Li, Wei; Ruan, Kangcheng

    2010-01-01

    The specific binding between Cy5-labeled goat anti-mouse Immunoglobulin G (IgG) and mouse IgG with a concentration range from 625 to 10 4 µg ml −1 has been detected successfully by the oblique-incidence reflectivity difference (OI-RD) method in each procedure of microarray fabrication. The experimental data prove that the OI-RD method can be employed not only to distinguish the different concentrations in label-free fashion but also to detect the antibody–antigen capture. In addition, the differential treatment of the OI-RD signals can decrease the negative influences of glass slide as the microarray upholder. Therefore the OI-RD technique has promising applications for the label-free and high-throughput detection of protein microarrays

  16. Spectral-Product Methods for Electronic Structure Calculations (Preprint)

    National Research Council Canada - National Science Library

    Langhoff, P. W; Mills, J. E; Boatz, J. A

    2006-01-01

    .... The spectral-product approach to molecular electronic structure avoids the repeated evaluations of the one- and two-electron integrals required in construction of polyatomic Hamiltonian matrices...

  17. Spectral-Product Methods for Electronic Structure Calculations (Postprint)

    National Research Council Canada - National Science Library

    Langhoff, P. W; Hinde, R. J; Mills, J. D; Boatz, J. A

    2007-01-01

    .... The spectral-product approach to molecular electronic structure avoids the repeated evaluations of the one- and two-electron integrals required in construction of polyatomic Hamiltonian matrices...

  18. Calculation of isotopic mass and energy production by a matrix operator method

    International Nuclear Information System (INIS)

    Lee, C.E.

    1976-08-01

    The Volterra method of the multiplicative integral is used to determine the isotopic density, mass, and energy production in linear systems. The solution method, assumptions, and limitations are discussed. The method allows a rapid accurate calculation of the change in isotopic density, mass, and energy production independent of the magnitude of the time steps, production or decay rates, or flux levels

  19. Modified Carbapenem Inactivation Method for Phenotypic Detection of Carbapenemase Production among Enterobacteriaceae.

    Science.gov (United States)

    Pierce, Virginia M; Simner, Patricia J; Lonsway, David R; Roe-Carpenter, Darcie E; Johnson, J Kristie; Brasso, William B; Bobenchik, April M; Lockett, Zabrina C; Charnot-Katsikas, Angella; Ferraro, Mary Jane; Thomson, Richard B; Jenkins, Stephen G; Limbago, Brandi M; Das, Sanchita

    2017-08-01

    The ability of clinical microbiology laboratories to reliably detect carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CP-CRE) is an important element of the effort to prevent and contain the spread of these pathogens and an integral part of antimicrobial stewardship. All existing methods have limitations. A new, straightforward, inexpensive, and specific phenotypic method for the detection of carbapenemase production, the carbapenem inactivation method (CIM), was recently described. Here we describe a two-stage evaluation of a modified carbapenem inactivation method (mCIM), in which tryptic soy broth was substituted for water during the inactivation step and the length of this incubation was extended. A validation study was performed in a single clinical laboratory to determine the accuracy of the mCIM, followed by a nine-laboratory study to verify the reproducibility of these results and define the zone size cutoff that best discriminated between CP-CRE and members of the family Enterobacteriaceae that do not produce carbapenemases. Bacterial isolates previously characterized through whole-genome sequencing or targeted PCR as to the presence or absence of carbapenemase genes were tested for carbapenemase production using the mCIM; isolates with Ambler class A, B, and D carbapenemases, non-CP-CRE isolates, and carbapenem-susceptible isolates were included. The sensitivity of the mCIM observed in the validation study was 99% (95% confidence interval [95% CI], 93% to 100%), and the specificity was 100% (95% CI, 82% to 100%). In the second stage of the study, the range of sensitivities observed across nine laboratories was 93% to 100%, with a mean of 97%; the range of specificities was 97% to 100%, with a mean of 99%. The mCIM was easy to perform and interpret for Enterobacteriaceae , with results in less than 24 h and excellent reproducibility across laboratories. Copyright © 2017 American Society for Microbiology.

  20. Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways

    DEFF Research Database (Denmark)

    2016-01-01

    The present invention generally relates to the microbiological industry, and specifically to the production of L-serine using genetically modified bacteria. The present invention provides genetically modified microorganisms, such as bacteria, wherein the expression of genes encoding for enzymes...... concentrations of serine. The present invention also provides methods for the production of L-serine or L-serine derivative using such genetically modified microorganisms....