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Sample records for macrophyte water spinach

  1. Response of littoral macrophytes to water level fluctuations in a storage reservoir

    OpenAIRE

    Krolová M.; Čížková H.; Hejzlar J.; Poláková S.

    2013-01-01

    Lakes and reservoirs that are used for water supply and/or flow regulations have usually poorly developed littoral macrophyte communities, which impairs ecological potential in terms of the EU Water Framework Directive. The aim of our study was to reveal controlling factors for the growth of littoral macrophytes in a storage reservoir with fluctuating water level (Lipno Reservoir, Czech Republic). Macrophytes occurred in this reservoir only in the eulittoral zone i.e., the shoreline region be...

  2. Response of littoral macrophytes to water level fluctuations in a storage reservoir

    OpenAIRE

    Krolová M.; Čížková H.; Hejzlar J.; Poláková S.

    2013-01-01

    Lakes and reservoirs that are used for water supply and/or flow regulations have usually poorly developed littoral macrophyte communities, which impairs ecological potential in terms of the EU Water Framework Directive. The aim of our study was to reveal controlling factors for the growth of littoral macrophytes in a storage reservoir with fluctuating water level (Lipno Reservoir, Czech Republic). Macrophytes occurred in this reservoir only in the eulittoral zone i.e., the shoreline region be...

  3. Macrophytes: Limitations of Using Them to Assess Reservoir Status According to the Water Framework Directive

    OpenAIRE

    Alaoui, Khadija Sossey; Galoux, Daniel; Rosillon, Francis

    2014-01-01

    Macrophytes are among the major groups of organisms that the Water Framework Directive (WFD) recommends should be used in assessing the status of natural lakes. The use of macrophytes in reservoir monitoring is still limited and further studies are needed on their inter-calibration and sources of variation. Many status assessment methods based on macrophyte communities have been defined for lakes. Nevertheless, few of them have been tested for reservoirs. The purpose of the study is to hig...

  4. Influence of nutrient level on methylmercury content in water spinach.

    Science.gov (United States)

    Greger, Maria; Dabrowska, Beata

    2010-08-01

    Widely consumed vegetables are often cultivated in sewage waters with high nutrient levels. They can contain high levels of methylmercury (MeHg), because they can form MeHg from inorganic Hg in their young shoots. We determined whether the MeHg uptake and the MeHg formation in the shoots of water spinach (Ipomoea aquatica) were affected by the presence of a high nutrient level in the growth medium. Water spinach shoots were rooted and pretreated in growth medium containing 7% (low) or 70% (high) Hoagland nutrient solution; thereafter, the plants were treated with either 0.02 microM MeHg or 0.2 microM HgCl2 for 3 d. Half the plants were then analyzed for total Hg and MeHg. The remaining plants were transferred to mercury-free medium with low or high nutrient levels and posttreated for 3 days before analysis of total Hg and MeHg in order to measure MeHg formation in the absence of external Hg. The results indicate that nutrient level did not influence MeHg uptake, but that a high nutrient level reduced the distribution of MeHg to the shoots 2.7-fold versus low nutrient level. After treatment with HgCl2, MeHg was found in roots and new shoots but not in old shoots. The MeHg:total-Hg ratio was higher in new shoots than in roots, being 13 times higher at high versus low nutrient levels. Thus, MeHg formation was the same in new shoots independent of inorganic Hg concentration, since the total Hg level decreased at a high nutrient level.

  5. Extending one-dimensional models for deep lakes to simulate the impact of submerged macrophytes on water quality

    NARCIS (Netherlands)

    Sachse, R.; Petzoldt, T.; Blumstock, M.; Moreira, S.; Pätzig, M.; Rücker, J.; Janse, J.H.; Mooij, W.M.; Hilt, S.

    2014-01-01

    Submerged macrophytes can stabilise clear water conditions in shallow lakes. However, many existing models for deep lakes neglect their impact. Here, we tested the hypothesis that submerged macrophytes can affect the water clarity in deep lakes. A one-dimensional, vertically resolved macrophyte mode

  6. Response of littoral macrophytes to water level fluctuations in a storage reservoir

    National Research Council Canada - National Science Library

    Krolová, M; Čížková, H; Hejzlar, J; Poláková, S

    2013-01-01

    Lakes and reservoirs that are used for water supply and/or flow regulations have usually poorly developed littoral macrophyte communities, which impairs ecological potential in terms of the EU Water Framework Directive...

  7. Impacts of aquatic macrophytes configuration modes on water quality.

    Science.gov (United States)

    Liu, Jiakai; Liu, Jinglan; Zhang, Rong; Zou, Yuqi; Wang, Huihui; Zhang, Zhenming

    2014-01-01

    Constructed wetland technology is regarded as an important ecological restoration technology and used widely in sewage disposal. In order to give them a wider scope of application and to improve their performance in water restoration, the current experiment was designed. Four aquatic macrophytes (dwarf cattail (TM), yellow-flowered iris (WI), water shallot (ST) and watermifoil (MS)) were picked and planted in artificial floating islands (AFIs) in different configurations (TM + WI, ST + MS and TM + WI + MS) and two patterns, radiation pattern (RP) and annular pattern (AP), for a 60-day experiment. Then, water quality and growth were monitored every 10 days. The results indicate that the different configurations performed diversely on waste water purification. First, a composite plant configuration removed more pollutant than a single one with the same total increment of biomass. Second, the plant configuration of MS + ST was most effective in total nitrogen (TN), total phosphorus (TP) or PO4(3-) removal, and TM + IW + MS was good at chemical oxygen demand (COD) and NO3(-) removal. However, different patterns comprised from the same species had a certain effect on absorption of pollutants. Generally speaking, plant configurations with a RP were better than an AP in purification. Accordingly, these provided the methods for the pollution wetland restoration.

  8. Nutritional Composition of Water Spinach (Ipomoea aquatica Forsk.) Leaves

    Science.gov (United States)

    Umar, K. J.; Hassan, L. G.; Dangoggo, S. M.; Ladan, M. J.

    Analyses of the nutritional composition of water spinach (Ipomoea aquatica) Forsk leaves were carried out using standard methods of food analysis. The proximate composition as well as mineral elements were determined. The leaves were found on dry weight basis to have high moisture (72.83±0.29%), ash (10.83±0.80%), crude lipid (11.00±0.50%), crude fibre (17.67±0.35%) and available carbohydrate (54.20±0.68%), but low in crude protein content (6.30±0.27%). The leaves also have energy value (300.94±5.31 kcal/100 g) that is within the range reported in some Nigerian leafy vegetables. The mineral element contents were high with remarkable concentration of K (5,458.33±954.70 mg/100 g) and Fe (210.30±2.47 mg/100 g). Also the leaves content moderate concentrations of Na (135.00±2.50 mg/100 g), calcium (416.70±5.77 mg/100 g), Magnesium (301.64±12.69 mg/100 g) and P (109.29±0.55 mg/100 g), with low Cu (0.36±0.01 mg/100 g), Mn (2.14±0.22 mg/100 g) and Zn (2.47±0.27 mg/100 g) contents. Comparing the mineral content with recommended dietary allowance, it was showed that the plant leaves is good sources of K, Mn and Fe for all categories of people, while Mg is adequate enough for adult female and children. From the result, Ipomoea aquatica Forsk leaves could be used for nutritional purposes, due to the amount and diversity of nutrients it contains.

  9. Towards Deriving Renewable Energy from Aquatic Macrophytes Polluting Water Bodies in Niger Delta Region of Nigeria

    Directory of Open Access Journals (Sweden)

    Badmus Abdurrahman Adeleye

    2013-01-01

    Full Text Available This study was performed to derive methane rich biogas from biomass of harvested water hyacinth polluting water bodies in selected rivers of the Niger delta region of Nigeria. Field visits were undertaken on selected rivers in the Niger Delta region in which aquatic macrophytes were collected and inventorized. Also different types of aquatic macrophytes were surveyed. Control by harvesting macrophytes and deriving energy (methane-rich biogas from biomass of one (water hyacinth was successfully carried out in this study. An initial test was conducted to evaluate methane rich biogas production from water hyacinth collected from the wild. After a successful production of combustible biogas, laboratory experiments aimed at generating biogas from harvested biomass of aquatic mycrophyte (water hyacinth cultivated under eutrophic and oligotrophic conditions were undertaken in the laboratory. The result of the study showed highest biogas yield of 22 L over a 40 day retention time for water hyacinth raised under eutrophic conditions. Biogas yield for water hyacinth raised under oligotrophic conditions recorded the highest yield of 53L over an 11 day retention time. The conversion of the biomass of harvested aquatic macrophyte (water hyacinth from the Niger Delta into renewable energy, that is combustible biogas, demonstrated an inevitable option for the control and management of environmental pollution associated with aquatic macrophytes and their usability for poverty alleviation in the Niger Delta region of Nigeria.

  10. Vegetative Propagule Pressure and Water Depth Affect Biomass and Evenness of Submerged Macrophyte Communities.

    Science.gov (United States)

    Li, Hong-Li; Wang, Yong-Yang; Zhang, Qian; Wang, Pu; Zhang, Ming-Xiang; Yu, Fei-Hai

    2015-01-01

    Vegetative propagule pressure may affect the establishment and structure of aquatic plant communities that are commonly dominated by plants capable of clonal growth. We experimentally constructed aquatic communities consisting of four submerged macrophytes (Hydrilla verticillata, Ceratophyllum demersum, Elodea nuttallii and Myriophyllum spicatum) with three levels of vegetative propagule pressure (4, 8 and 16 shoot fragments for communities in each pot) and two levels of water depth (30 cm and 70 cm). Increasing vegetative propagule pressure and decreasing water level significantly increased the growth of the submerged macrophyte communities, suggesting that propagule pressure and water depth should be considered when utilizing vegetative propagules to re-establish submerged macrophyte communities in degraded aquatic ecosystems. However, increasing vegetative propagule pressure and decreasing water level significantly decreased evenness of the submerged macrophyte communities because they markedly increased the dominance of H. verticillata and E. nuttallii, but had little impact on that of C. demersum and M. spicatum. Thus, effects of vegetative propagule pressure and water depth are species-specific and increasing vegetative propagule pressure under lower water level can facilitate the establishment success of submerged macrophyte communities.

  11. Species pool versus site limitations of macrophytes in urban waters

    DEFF Research Database (Denmark)

    Vermonden, K.; Leuven, R.S.E.W.; van der Velde, G.

    2010-01-01

    Biodiversity in urban areas is affected by a multitude of stressors. In addition to physico-chemical stress factors, the native regional species pool can be greatly reduced in highly urbanized landscapes due to area loss and fragmentation. In this study, we investigated how macrophyte composition...

  12. Element contents and food safety of water spinach (Ipomoea aquatica Forssk.) cultivated with wastewater in Hanoi, Vietnam

    DEFF Research Database (Denmark)

    Marcussen, Helle; Jørgensen, Karin; Holm, Peter E.;

    2008-01-01

    Extensive aquatic or semi-aquatic production of water spinach (Ipomoea aquatica Forssk.) for human consumption takes place in Southeast Asia. The aim of this study was to assess the concentrations of 38 elements in soil and water spinach cultivated under different degrees of wastewater exposure...... in Hanoi, Vietnam. The results showed no effect of wastewater use on the overall element concentrations in soil and water spinach. Mean soil concentrations for selected potentially toxic elements at the studied field sites had the following ranges 9.11-18.7 As, 0.333-0.667 Cd, 10.8-14.5 Co, 68-122 Cr, 34...

  13. Predicting aquatic macrophyte occurrence in soft-water oligotrophic lakes (Pyrenees mountain range

    Directory of Open Access Journals (Sweden)

    Cristina Pulido

    2014-08-01

    Full Text Available Distribution of aquatic macrophytes in lakes is related to geographical, morphological, catchment and water chemistry variables as well as human impacts, which modify the original environment. Here, we aim at building statistical models to establish the ecological niches of 11 aquatic macrophytes (10 different phanerogams and the genus Nitella from oligotrophic soft-water lakes and infer their ecological requirements and environmental constraints at the southernmost limit of their distribution. Macrophyte occurrence and environmental variables were obtained from 86 non-exploited oligotrophic soft-water lakes from the Pyrenees (Southern Europe; 42º50´N, 1º00´E; macrophytes inhabited 55 of these lakes. Optimum ranges and macrophyte occurrence were predicted in relation to 18 geographical, morphological, catchment and water chemistry variables using univariate and multivariate logistic models. Lakes at low altitude, in vegetated catchments and with low water concentration of NO3- and SO4-2, were the most suitable to host macrophytes. In general, individual species of aquatic macrophytes showed clear patterns of segregation along conductivity and pH gradients, although the specific combination of variables selected in the best models explaining their occurrence differed among species.  Based on the species response to pH and conductivity, we found Isoetes lacustris have its optimum in waters with low conductivity and pH (i.e. negative monotonic response. In contrast, Callitriche palustris, Ranunculus aquatilis, Subularia aquatica, Nitella spp., and Myriophyllum alterniflorum showed an optimum at intermediate values (i.e. unimodal response, whereas Potamogeton berchtoldii, Potamogeton alpinus, and Ranunculus trichophyllus as species had their optimum at relatively high water pH and conductivity (i.e. positive monotonic response. This pattern has been observed in other regions for the same species, although with different optima and tolerance

  14. Response of littoral macrophytes to water level fluctuations in a storage reservoir

    Directory of Open Access Journals (Sweden)

    Krolová M.

    2013-05-01

    Full Text Available Lakes and reservoirs that are used for water supply and/or flow regulations have usually poorly developed littoral macrophyte communities, which impairs ecological potential in terms of the EU Water Framework Directive. The aim of our study was to reveal controlling factors for the growth of littoral macrophytes in a storage reservoir with fluctuating water level (Lipno Reservoir, Czech Republic. Macrophytes occurred in this reservoir only in the eulittoral zone i.e., the shoreline region between the highest and the lowest seasonal water levels. Three eulittoral sub-zones could be distinguished: the upper eulittoral with a stable community of perennial species with high cover, the middle eulittoral with relatively high richness of emergent and amphibious species present at low cover values, and the lower eulittoral devoid of permanent vegetation. Cover and species composition in particular sub-zones were primarily influenced by the duration and timing of flooding, followed by nutrient limitation and strongly reducing conditions in the flooded organic sediment. Our results stress the ecological importance of eulittoral zone in reservoirs with fluctuating water levels where macrophyte growth can be supported by targeted management of water level, thus helping reservoir managers in improving the ecological potential of this type of water bodies.

  15. Management of fresh water weeds (macrophytes) by vermicomposting using Eisenia fetida.

    Science.gov (United States)

    Najar, Ishtiyaq Ahmed; Khan, Anisa B

    2013-09-01

    In the present study, potential of Eisenia fetida to recycle the different types of fresh water weeds (macrophytes) used as substrate in different reactors (Azolla pinnata reactor, Trapa natans reactor, Ceratophyllum demersum reactor, free-floating macrophytes mixture reactor, and submerged macrophytes mixture reactor) during 2 months experiment is investigated. E. fetida showed significant variation in number and weight among the reactors and during the different fortnights (P weeds) into three clusters-poor vermicompost substrates, moderate vermicompost substrate, and excellent vermicompost substrate. Two principal components (PCs) have been identified by factor analysis with a cumulative variance of 90.43 %. PC1 accounts for 47.17 % of the total variance represents "reproduction factor" and PC2 explaining 43.26 % variance representing "growth factor." Thus, the nature of macrophyte affects the growth and reproduction pattern of E. fetida among the different reactors, further the addition of A. pinnata in other macrophytes reactors can improve their recycling by E. fetida.

  16. The effect of sewage sludge application on the growth and absorption rates of Pb and As in water spinach

    Directory of Open Access Journals (Sweden)

    Rong Wang

    2016-01-01

    Full Text Available This paper investigated the effect of the application of sewage sludge on the growth rates and absorption rates of Pb and As in potted water spinach. Our results indicated that application of sewage sludge promoted vegetable growth, and the dry weight of water spinach reached a maximal value (4.38 ± 0.82 g upon 8% sludge application. We also found that the dry weights of water spinach after treatment were all greater than those of the control systems (CK. Treatment with sludge promoted the absorption of Pb and As in water spinach, with a significant (p < 0.05 increase of absorbed Pb following treatment concentrations above 10%, and a peak absorption of As at 8%. Finally, we found that concentrations of Pb and As were higher in rhizosphere-attached soil than in free pot.

  17. Heavy metals phyto-assessment in commonly grown vegetables: water spinach (I. aquatica) and okra (A. esculentus).

    Science.gov (United States)

    Ng, Chuck Chuan; Rahman, Md Motior; Boyce, Amru Nasrulhaq; Abas, Mhd Radzi

    2016-01-01

    The growth response, metal tolerance and phytoaccumulation properties of water spinach (Ipomoea aquatica) and okra (Abelmoschus esculentus) were assessed under different contaminated spiked metals: control, 50 mg Pb/kg soil, 50 mg Zn/kg soil and 50 mg Cu/kg soil. The availability of Pb, Zn and Cu metals in both soil and plants were detected using flame atomic absorption spectrometry. The concentration and accumulation of heavy metals from soil to roots and shoots (edible parts) were evaluated in terms of translocation factor, accumulation factor and tolerance index. Okra recorded the highest accumulation of Pb (80.20 mg/kg) in its root followed by Zn in roots (35.70 mg/kg) and shoots (34.80 mg/kg) of water spinach, respectively. Different accumulation trends were observed with, Pb > Zn > Cu in okra and Zn > Pb > Cu in water spinach. Significant differences (p < 0.01) of Pb, Zn and Cu accumulation were found in both water spinach and okra cultivated among tested treatments. However, only the accumulation of Pb metal in the shoots of water spinach and okra exceeded the maximum permissible levels of the national Malaysian Food Act 1983 and Food Regulations 1985 (2006) as well as the international Codex Alimentarius Commission limits. This study has shown that both water spinach and okra have good potential as Pb and Zn phytoremediators.

  18. Moderate water stress prevents the postharvest decline of ascorbic acid in spinach (Spinacia oleracea L.) but not in spinach beet (Beta vulgaris L.).

    Science.gov (United States)

    Mogren, Lars M; Beacham, Andrew M; Reade, John P H; Monaghan, James M

    2016-07-01

    Babyleaf salads such as spinach (Spinacia oleracea L.) and spinach beet (Beta vulgaris L. subsp. cicla var. cicla) are an important dietary source of antioxidants such as ascorbic acid (vitamin C). Such compounds may be important in disease prevention in consumers but the level of these compounds in leaves frequently declines after harvest. As such, methods to maintain antioxidant levels in fresh produce are being sought. Irrigation deficits were used to apply water stress to S. oleracea and B. vulgaris plants. This treatment prevented postharvest decline of leaf ascorbic acid content in S. oleracea but not in B. vulgaris. Ascorbic acid levels in leaves at harvest were unaffected by the treatment in both species compared to well-watered controls. We have shown that restricted irrigation provides a viable means to maintain leaf vitamin content after harvest in S. oleracea, an important finding for producers, retailers and consumers alike. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  19. Uptake and transport of roxarsone and its metabolites in water spinach as affected by phosphate supply.

    Science.gov (United States)

    Yao, Lixian; Li, Guoliang; Dang, Zhi; Yang, Baomei; He, Zhaohuan; Zhou, Changmin

    2010-04-01

    Roxarsone (ROX) is widely used as a feed additive in intensive animal production. While an animal is fed with ROX, the As compounds in the manure primarily occur as ROX and its metabolites, including arsenate (As[V]), arsenite (As[III]), monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA). Animal manure is commonly land applied with phosphorous fertilizers in China. A pot experiment was conducted to investigate the phytoavailability of ROX, As(V), As(III), MMA, and DMA in water spinach (Ipomoea aquatica), with the soil amended with 0, 0.25, 0.50, 1.0, and 2.0 g PO(4)/kg, respectively, plus 2% (w/w manure/soil) chicken manure (CM) bearing ROX and its metabolites. The results indicate that this species of water spinach cannot accumulate ROX and MMA at detectable levels, but As(V), As(III), and DMA were present in all plant samples. Increased phosphorous decreased the shoot As(V) and As(III) in water spinach but did not affect the root As(V). The shoot DMA and root As(III) and DMA were decreased/increased and then increased/decreased by elevated phosphorous. The total phosphorous content (P) in plant tissue did not correlate with the total As or the three As species in tissues. Arsenate, As(III), and DMA were more easily accumulated in the roots, and phosphate considerably inhibited their upward transport. Dimethylarsinic acid had higher transport efficiency than As(V) and As(III), but As(III) was dominant in tissues. Conclusively, phosphate had multiple effects on the accumulation and transport of ROX metabolites, which depended on their levels. However, proper utilization of phosphate fertilizer can decrease the accumulation of ROX metabolites in water spinach when treated with CM containing ROX and its metabolites.

  20. Do Amplitudes of Water Level Fluctuations Affect the Growth and Community Structure of Submerged Macrophytes?

    Science.gov (United States)

    Wang, Mo-Zhu; Liu, Zheng-Yuan; Luo, Fang-Li; Lei, Guang-Chun; Li, Hong-Li

    2016-01-01

    Submerged macrophytes are subjected to potential mechanical stresses associated with fluctuating water levels in natural conditions. However, few experimental studies have been conducted to further understand the effects of water level fluctuating amplitude on submerged macrophyte species and their assemblages or communities. We designed a controlled experiment to investigate the responses of three submerged macrophyte species (Hydrilla verticillata, Ceratophyllum demersum and Elodea nuttallii) and their combinations in communities to three amplitudes (static, ± 30 cm, ± 60 cm) of water level fluctuations. Results showed that water level fluctuating amplitude had little effects on the community performance and the three tested species responded differently. H. verticillata exhibited more growth in static water and it was negatively affected by either of the water level fluctuations amplitude, however, growth parameters of H. verticillata in two fluctuating water level treatments (i.e., ± 30 cm, ± 60 cm) were not significantly different. On the other hand, the growth of C. demersum was not significantly correlated with different amplitude treatments. However, it became more abundant when water levels fluctuated. E. nuttallii was inhibited by the two fluctuating water level treatments, and was less in growth parameters compared to the other species especially in water level fluctuating conditions. The inherent differences in the adaptive capabilities of the tested species indicate that C. demersum or other species with similar responses may be dominant species to restore submerged macrophyte communities with great fluctuating water levels. Otherwise, H. verticillata, E. nuttallii or other species with similar responses could be considered for constructing the community in static water conditions.

  1. Water quality and communities associated with macrophytes in a shallow water-supply reservoir on an aquaculture farm.

    Science.gov (United States)

    Sipaúba-Tavares, L H; Dias, S G

    2014-05-01

    Plankton communities and macrofauna associated to aquatic macrophyte stands in a shallow water-supply reservoir (21°14'09″S; 48°18'38″W) on an aquaculture farm were compared to evaluate the relationship between organism densities and some abiotic features of the reservoir. Water and communities associated were sampled at two sites, one in an area with the predominance of Eichhornia azurea (Sw.) Kunth and the other with the predominance of Salvinia auriculata Aublet. Communities associated with macrophytes were sampled with floating quadrants (0.5 m2); the macrophytes were washed and plankton and macrofauna were fixated with 4% formalin and 1% lugol iodine; the specimens were then identified and counted. Plankton and macrofauna communities associated with S. auriculata and E. azurea had a similar diversity of species but different (pmacrophytes presence in the shallow reservoir is a strong predictor of favourable conditions to maintain great diversity plankton community and macrofauna associated with plants. The role of macrophytes is important for not only stabilising the clear-water state and maintaining high diversity of organisms associated, but also it seems to be a good alternative to maintaining desirable water-supply quality for aquaculture farms.

  2. Effect of pH Upper Control Limit on Nutrient Solution Component and Water Spinach Growth under Hydroponics

    Directory of Open Access Journals (Sweden)

    Xuzhang Xue

    2015-09-01

    Full Text Available In this study, experiment with four levels of nutrient solution pH control upper limit was conducted to explore the optimal nutrient solution pH management scheme under hydroponics by evaluating the nutrient solution characters i.e., pH, Electric Conductivity (EC, nitrate, soluble phosphorus (soluble-P, water spinach growth and quality. The results showed that the nutrient solution pH was 8.2 and unsuitable for water spinach growth under the treatment with no pH regulation during the experimental period. The pH could be maintained within a reasonable range for water spinach growth by adding nitric acid to nutrient solution. Meanwhile, the availability of cations and soluble-P were improved. The water/nutrition uptake was also promoted. Through nutrient solution pH control, the plant height, leaf number and fresh/dry shoot weight increased by 34.66-55.70%, 12.42-13.66%, 39.18¬-101.72% and 13.78-74.03%, respectively. It indicated that pH regulation could improve water spinach growth, promote allocation of photosynthetic product to shoot and increase the effective yield. The nitrate content of water spinach shoot increased, but which was lower than the critical value of 3000 mg/kg FW. Vitamin C content decreased under the treatment which 6.5 of pH control upper limit was set. There were no significant differences in soluble sugar and crude protein content among the four treatments. Considering the yield and quality of water spinach, the suitable nutrient solution pH control upper limit was 7.0.

  3. Faecal and protozoan parasite contamination of water spinach (Ipomoea aquatica) cultivated in urban wastewater in Phnom Penh, Cambodia

    DEFF Research Database (Denmark)

    Anh, Vuong Tuan; Tram, Nguyen Thuy; Klank, Lise Tønner

    2007-01-01

    Objective To identify the level of contamination with thermotolerant coliforms (ThC), intestinal helminth eggs and protozoan parasites in water spinach (Ipomoea aquatica) cultivated in a wastewater-fed lake in Phnom Penh, Cambodia. Methods The investigation was carried out from July 2004 to May...... into a stream. Water spinach samples were harvested at each of the three locations with and without wastewater contact according to the normal practices of farmers, and analysed for ThC, protozoan parasites (Giardia, Cryptosporidium and Cyclospora), and helminth eggs (Ascaris lumbricoides, Trichuris trichura...... and hookworm) using standard methods. Wastewater samples were also collected at each of the three locations and analysed for ThC and helminth eggs. Results High concentrations of ThC (approximately 10(5)-10(7)/g) were found in water spinach samples. ThC mean counts did not differ significantly, neither between...

  4. Water quality and communities associated with macrophytes in a shallow water-supply reservoir on an aquaculture farm

    Directory of Open Access Journals (Sweden)

    LH. Sipaúba-Tavares

    Full Text Available Plankton communities and macrofauna associated to aquatic macrophyte stands in a shallow water-supply reservoir (21°14′09″S; 48°18′38″W on an aquaculture farm were compared to evaluate the relationship between organism densities and some abiotic features of the reservoir. Water and communities associated were sampled at two sites, one in an area with the predominance of Eichhornia azurea (Sw. Kunth and the other with the predominance of Salvinia auriculata Aublet. Communities associated with macrophytes were sampled with floating quadrants (0.5 m2; the macrophytes were washed and plankton and macrofauna were fixated with 4% formalin and 1% lugol iodine; the specimens were then identified and counted. Plankton and macrofauna communities associated with S. auriculata and E. azurea had a similar diversity of species but different (p<0.05 in the abundance of associated organisms. Eichhornia azurea had the highest contents in dry and wet weight, total phosphorus, total nitrogen and organic matter. Planktonic algae were directly correlated with biomass of E. azurea. The taxa with highest densities were Rotifera and Zygnematophyceae. Results showed that the environmental variables associated with macrophytes presence in the shallow reservoir is a strong predictor of favourable conditions to maintain great diversity plankton community and macrofauna associated with plants. The role of macrophytes is important for not only stabilising the clear-water state and maintaining high diversity of organisms associated, but also it seems to be a good alternative to maintaining desirable water-supply quality for aquaculture farms.

  5. Kinetics of Cr(III) and Cr(VI) removal from water by two floating macrophytes.

    Science.gov (United States)

    Maine, M A; Hadad, H R; Sánchez, G; Caffaratti, S; Pedro, M C

    2016-01-01

    The aim of this work was to compare Cr(III) and Cr(VI) removal kinetics from water by Pistia stratiotes and Salvinia herzogii. The accumulation in plant tissues and the effects of both Cr forms on plant growth were also evaluated. Plants were exposed to 2 and 6 mg L(-1) of Cr(III) or Cr(VI) during 30 days. At the end of the experiment, Cr(VI) removal percentages were significantly lower than those obtained for Cr(III) for both macrophytes. Cr(III) removal kinetics involved a fast and a slow component. The fast component was primarily responsible for Cr(III) removal while Cr(VI) removal kinetics involved only a slow process. Cr accumulated principally in the roots. In the Cr(VI) treatments a higher translocation from roots to aerial parts than in Cr(III) treatments was observed. Both macrophytes demonstrated a high ability to remove Cr(III) but not Cr(VI). Cr(III) inhibited the growth at the highest studied concentration of both macrophytes while Cr(VI) caused senescence. These results have important implications in the use of constructed wetlands for secondary industrial wastewater treatment. Common primary treatments of effluents containing Cr(VI) consists in its reduction to Cr(III). Cr(III) concentrations in these effluents are normally below the highest studied concentrations in this work.

  6. The role of herbivorous water birds in aquatic systems through interactions with aquatic macrophytes, with special reference to the Bewick's Swan - Fennel Pondweed system

    NARCIS (Netherlands)

    Klaassen, M.R.J.; Nolet, B.A.

    2007-01-01

    The role of aquatic macrophytes in stimulating biodiversity and maintaining clear waters is currently undisputed. The management of (eutrophic) shallow waters is therefore often directed at (re-)establishing macrophyte domination. In contrast, the role of water birds has long been considered of

  7. Persistence and distribution of 4-nonylphenol in water, sediment, macrophytes, and wall material of littoral enclosures

    Energy Technology Data Exchange (ETDEWEB)

    Heinis, L.J.; Tunell, R.; Liber, K. [Univ. of Wisconsin, Superior, WI (United States). Lake Superior Research Inst.; Knuth, M.L. [Environmental Protection Agency, Duluth, MN (United States). Environmental Research Lab.

    1994-12-31

    Eighteen enclosures (5 m x 10 m) were constructed in the littoral zone of a 2-ha pond near Duluth, MN. Each enclosure consisted of 5 m of natural shoreline and three walls of an inert plastic. The enclosures had an average surface area of 31.9 m{sup 2} , an average depth of 0.6 m and an average water volume of 33.1 m{sup 3}. The enclosure waters were treated with the alkyl phenol ethoxylate precursor and degradation product 4-nonylphenol. Application was accomplished by sub-surface injection over a 20-day period with a 2 day frequency. Nominal aqueous concentrations were 0, 3, 30, 100 and 300 {mu}g/L. Concentrations of 4-nonylphenol were monitored during and after application in the water, sediment, macrophytes, and enclosure wall material. Average maximum water concentrations ranged from 96.5% of nominal to 62.0% of nominal and average minimum water concentrations ranged from 33.3% of nominal to 29.5% of nominal during the application period. Water concentrations decreased exponentially after application ended. Sediment concentrations during the application period were constant from 8 to 20 d and peak concentrations occurred 48 d after application began. Macrophyte concentrations peaked 21 d after initial application with a steady decline through 76 d. Enclosure wall material concentrations reached a peak 3 h before the final application. A gradual decline occurred until 34 d after initial application followed by a more rapid dissipation.

  8. UVR induce optical changes and phosphorous release of lake water and macrophyte leachates in shallow Andean lakes

    Directory of Open Access Journals (Sweden)

    Beatriz MODENUTTI

    2010-02-01

    Full Text Available We carried out laboratory experiments in order to study the effect of ultraviolet radiation (UVR on optical features and phosphorous release of Dissolved Organic Mater (DOM from lake water and macrophyte leachates. Lake water samples were obtained from lakes Escondido and El Trébol, and macrophytes (Potamogeton linguatus and Schoenoplectus californicus from their littoral zones. After UVR exposure, DOM from lake El Trébol seemed to react more quickly than that from Lake Escondido and this seems to be related with the degree of lability or aromaticity in the DOM bulk of each lake. Leachates from both macrophytes showed different absorbance spectra with differences in photochemical transformations after UVR exposure: S. californicus leachates exhibited the highest photodegradation. A significant Soluble Reactive Phosphorus (SRP release was observed in lake water after UVR exposure. Lake El Trébol showed the highest SRP concentrations, suggesting that the release of orthophosphate was favored by low molecular weight DOM. P. linguatus leachates have more dissolved phosphorus content than S. californicus ones and after UVR exposure, P. linguatus leachate did not react to UVR while S. californicus exhibited a decrease in SRP. However both macrophyte leachates showed the higher P release in darkness. The obtained results indicated that macrophyte leachates could contribute significantly to changes in the optical characteristics and in the nutrient content in shallow Andean lakes. An increasing input of P. linguatus leachates would produce DOM of high molecular size and a higher P release than S. californicus.

  9. Effects of Water and Nitrogen Supply on Spinach(Spinacia oleracea L.) Growth and SoilMineral N Residues

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Effects of conventional and optimized water and nitrogen managements on spinach (Spinacia oleracea L.)growth and soil mineral N (Nmin) residues were compared in an open field experiment in which water balance method and N recommendation with the KNS-system were included. It was shown that the conventional water treatment (seasonal irrigated amount: 175 mm) reduced spinach growth compared to the water balance treatments (seasonal irrigated amount: 80 and 85 mm) at the same N supply level due to N loss through leaching caused by excessive water supply. Although 309 kg N ha-1 was applied in the conventional N treatment, compared to 82 and 66 kg N ha-1 in the optimum N treatments, no significant difference in crop yield was investigated between the N treatments with the same irrigation practice. N uptake in spinach and soil residual Nmin contents were also significantly affected by the irrigation practices. The conventional water supply not only decreased water use efficiency, but also resulted in excessive NO-3-N being leached below the root zone. In order to meet the same target value of N requirement for the next crop, cauliflower, based on the KNS-system, at least extra 50 kg N ha-1 was needed in the conventional water treatments in comparison to the water balance treatment.

  10. EUTROPHICATION OF WATER RESERVOIRS AND ROLE OF MACROPHYTES IN THIS PROCESS

    Directory of Open Access Journals (Sweden)

    Joanna Jadwiga Sender

    2017-06-01

    Full Text Available The paper presents the problem related with the process of eutrophication, with special emphasis on dam reservoirs. Eutrophication is a global process, threatening the water ecosystem on every continent. It often leads to their degradation. Particularly vulnerable to eutrophication are artificial reservoirs which are dam reservoirs. This paper describes the mechanisms of eutrophication. We also pointed to the importance of aquatic plants in the process of water purification, as well as the possibility of multilateral use. Recently, in the world and in Poland there is a tendency to pay attention to the natural or semi-natural method of water purification (including constructed wetland. On the one hand, the presence of macrophytes in water bodies is a guarantor of good ecological status, on the other hand, the undeniable aesthetic value.

  11. Influence of emergent macrophyte (Phragmites australis) density on water turbulence and erosion of organic-rich sediment

    Institute of Scientific and Technical Information of China (English)

    HORPPILA Jukka; KAITARANTA Joni; JOENSUU Laura; NURMINEN Leena

    2013-01-01

    The effects of the emergent macrophyte Phragmites australis on water turbulence,bottom shear velocity and water turbidity were experimentally studied with the stem densities 0 stems/m2-100 stems/m2 and using organic-rich sediment.It is found that the maximum root-mean-square (rms) velocity and shear velocity decrease linearly with increasing stem density.By affecting the height of peak turbulence values,emergent plants can thus lower the frequency of sediment erosion events.Additionally,the slope of the rms turbulence increment decreases with increasing stem density.Thus,also the duration of strong turbulence is affected by the density of emergent macrophytes.The critical shear velocity for sediment resuspension is 0.0035 rn/s-0.0055 rn/s and not affected by the stem density.In the absence of macrophytes,water flow causes a 6.3-fold increment in turbidity,but with macrophytes turbidity values remain lower.The experiments suggest that even relatively low densities of Phragmites can have substantial effects on water turbulence and consequently on water quality.

  12. Macrobenthos of Vembanad estuary in relation to the deposition of degraded water fern Salvinia and other macrophytes

    Digital Repository Service at National Institute of Oceanography (India)

    Gopalan, U.K.; Meenakshikunjamma, P.P.; Vengayil, D.T.

    Composition and seasonal abundance of macrobenthos in Vembanad estuary (India) seems to be influenced by the density of decaying macrophytes, especially the water fern Salvinia , deposited at the bottom of the estuary at the rate of 356.76 g/m super...

  13. Analysis of the impact of water level fluctuations on macrophytes in Miyun Reservoir after receiving water transferred by the South-to-North Water Diversion Project

    Science.gov (United States)

    Yu, L.; Gu, H.; Lou, C. H.; Zhang, L.; Meng, Q. Y.

    2016-08-01

    As the main primary producers in aquatic ecosystems, macrophytes affect the structure and function of aquatic ecosystems, and their distribution is controlled by water depth. Miyun Reservoir in Beijing will have to experience substantial changes in water level and surface area as it begins to receive water transferred by the South-to-North Water Diversion Project, which will have an adverse impact on the macrophytes growing there. In this study, a hydrodynamic model was constructed with MIKE21 and then used in a simulation in three scenarios: dry year, normal year and wet year. The results suggest that during water diversion, the annual and interannual water level fluctuations will be too significant for them to adapt and as a result, the original macrophytes in the reservoir tend to die and disappear completely. The area of the zone suitable for macrophyte growth, or suitable growth zone (SGZ), fluctuated. Restricted by the main dam and auxiliary dam to its south, the overall suitable growth zone moved toward the northeast and northwest of the reservoir, with a northeastward movement of its centroid. The distance and path of movement varied between scenarios. After the water diversion was completed, the suitable growth zone shrunk in the three scenarios. It is predicted that the macrophyte species diversity and richness of the reservoir can recover to the levels recorded before water diversion only in dry year. These results suggest that manual interventions should be implemented after water diversion to speed up the natural recovery of aquatic plant communities in Miyun Reservoir and thereby maintain the stability of the aquatic ecosystem.

  14. Field evidence of the influence of aquatic macrophytes on water quality in a shallow eutrophic lake over a 13-year period

    Directory of Open Access Journals (Sweden)

    Edélti Faria Albertoni

    2014-06-01

    Full Text Available AIM: The main objective of this work is to describe the changes in water characteristics of a shallow subtropical lake, in periods with and without growing of macrophytes, related to periods of clear-macrophyte dominance and turbid-phytoplankton dominance states. METHODS: The study was conducted in Biguás Lake, in the south coastal plain of Brazil (32° 04' 43" S and 52° 10' 03" W. Samplings were carried out monthly between October 2000 and November 2013. The limnological variables measured in the water column were dissolved oxygen (DO, water temperature, pH, electrical conductivity (EC, chlorophyll-a, total nitrogen (TN, total phosphorous (TP and suspended material (SM. Data were grouped according to periods with macrophyte growth dominance (MD and without macrophytes, with phytoplankton dominance (PD, and applied t- tests among TP, TN, Chlorophyll-a and SM. During macrophyte growth we estimated the coverage (% and biomass variation of plants. RESULTS: Over the 13 years, the lake was well oxygenated, alkaline, and with a temperature variation according to subtropical seasonality. The lower values of all of the limnological variables were verified during periods of macrophyte growth, characterizing periods of clear and turbid waters. CONCLUSIONS: The influence of aquatic macrophytes in improving water quality in this shallow lake during the studied period, reducing nutrient concentrations, chlorophyll-a and suspended material in water, favoring the maintenance of a clear water state, was verified.

  15. Simultaneous recovery of bacteria and viruses from contaminated water and spinach by a filtration method.

    Science.gov (United States)

    Brassard, Julie; Guévremont, Évelyne; Gagné, Marie-Josée; Lamoureux, Lisyanne

    2011-01-05

    Water and leafy vegetables eaten fresh are increasingly reported as being involved in food-borne illness cases. The pathogenic agents responsible for these infections are mainly bacteria and viruses and are present in very small quantities on the contaminated food matrices. Laboratory techniques used to isolate or detect the contaminating agent differ enormously according to the type of microorganisms, generating time and economical losses. The purpose of this study was to optimize a single method which allows at the same time the recovery and concentration of these two main types of pathogenic organisms. Water and spinach samples were artificially contaminated with the feline calicivirus (FCV), rotavirus, hepatitis A virus (HAV), Escherichia coli, Listeria monocytogenes, Campylobacter jejuni and Salmonella Typhimurium. The principle behind the recovery technique is based on the use of a positively charged membrane which adsorbs both viruses and bacteria present in the water or in the rinse from the vegetables. Using conventional microbiology, PCR and RT-PCR, this filtration technique allowed a detection level superior to 10² CFU/g for S. Typhimurium, E. coli, L. monocytogenes and C. jejuni and to 10¹ PFU/g for FCV, HAV and rotavirus. This combined method can also be applied to other bacterial and viral species for the identification of the responsible agent for food-borne illnesses. Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.

  16. Foliar uptake of cesium from the water column by aquatic macrophytes.

    Science.gov (United States)

    Pinder, J E; Hinton, T G; Whicker, F W

    2006-01-01

    The probable occurrence and rate of foliar absorption of stable cesium (133Cs) from the water column by aquatic macrophyte species was analyzed following the addition of 133Cs into a small reservoir near Aiken, South Carolina, USA. An uptake parameter u (10(3)Lkg(-1)d(-1)) and a loss rate parameter k (d(-1)) were estimated for each species using time series of 133Cs concentrations in the water and plant tissues. Foliar uptake, as indicated by rapid increases in plant concentrations following the 133Cs addition, occurred in two floating-leaf species, Brasenia schreberi and Nymphaea odorata, and two submerged species, Myriophyllum spicatum and Utricularia inflata. These species had values of u> or =0.75 x 10(3)Lkg(-1)d(-1). Less evidence for foliar uptake was observed in three emergent species, including Typha latifolia. Ratios of u to k for B. schreberi, M. spicatum, N. odorata and U. inflata can be used to estimate concentration ratios (CR) at equilibrium, and these estimates were generally within a factor of 2 of the CR for 137Cs for these species in the same reservoir. This correspondence suggests that foliar uptake of Cs was the principal absorption mechanism for these species. Assessments of: (1) the prevalence of foliar uptake of potassium, rubidium and Cs isotopes by aquatic macrophytes and (2) the possible importance of foliar uptake of Cs in other lentic systems are made from a review of foliar uptake studies and estimation of comparable u and k values from lake studies involving Cs releases.

  17. Foliar uptake of cesium from the water column by aquatic macrophytes

    Energy Technology Data Exchange (ETDEWEB)

    Pinder, J.E. [Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29801 (United States); Hinton, T.G. [Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29801 (United States)]. E-mail: thinton@srel.edu; Whicker, F.W. [Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523-1618 (United States)

    2006-07-01

    The probable occurrence and rate of foliar absorption of stable cesium ({sup 133}Cs) from the water column by aquatic macrophyte species was analyzed following the addition of {sup 133}Cs into a small reservoir near Aiken, South Carolina, USA. An uptake parameter u (10{sup 3} L kg{sup -1} d{sup -1}) and a loss rate parameter k (d{sup -1}) were estimated for each species using time series of {sup 133}Cs concentrations in the water and plant tissues. Foliar uptake, as indicated by rapid increases in plant concentrations following the {sup 133}Cs addition, occurred in two floating-leaf species, Brasenia schreberi and Nymphaea odorata, and two submerged species, Myriophyllum spicatum and Utricularia inflata. These species had values of u {>=} 0.75 x 10{sup 3} L kg{sup -1} d{sup -1}. Less evidence for foliar uptake was observed in three emergent species, including Typha latifolia. Ratios of u to k for B. schreberi, M. spicatum, N. odorata and U. inflata can be used to estimate concentration ratios (CR) at equilibrium, and these estimates were generally within a factor of 2 of the CR for {sup 137}Cs for these species in the same reservoir. This correspondence suggests that foliar uptake of Cs was the principal absorption mechanism for these species. Assessments of: (1) the prevalence of foliar uptake of potassium, rubidium and Cs isotopes by aquatic macrophytes and (2) the possible importance of foliar uptake of Cs in other lentic systems are made from a review of foliar uptake studies and estimation of comparable u and k values from lake studies involving Cs releases.

  18. Co-occurrence between macrophytes and macroinvertebrates: towards a new approach for the running waters quality evaluation?

    Directory of Open Access Journals (Sweden)

    Lorenzo Traversetti

    2014-08-01

    Full Text Available Several studies on concordance between macrophyte and macroinvertebrate communities were carried out for decades while any investigation on co-occurrence of single pair of taxa of these two groups was never addressed. Our main aim was to verify the existence of co-occurrence of single macrophyte–macroinvertebrate pair in a Mediterranean river of central Italy. We compared the co-occurrence frequencies of different plant and animal taxa with those expected by chance through the null model approach. Specifically, we proposed two main co-occurrence patterns of macrophyte and macroinvertebrate taxa in terms of aggregation (when groups co-occur more frequently than expected and segregation (when groups co-occur less frequently than expected. This approach was supported by correlations and analysis of covariance in order to identify the most significant co-occurring pairs. In this study we collected a total of 123 taxa (50 macrophytes + 73 macroinvertebrates in October 2008 and 102 (42+60 in June 2009. We obtained a total of 379 significantly correlating macrophyte-macroinvertebrate pairs. Amongst all these correlating pairs, 83.7% of them correlated in October session while 90.3% in June. All the correlating pairs showed a high relationships with human alterations in both the sampling sessions. Our results confirmed that direct correlations there are between macrophytes and macroinvertebrates, highlighting how these two biological groups co-occur mainly following the aggregative model in both sampling sessions. Therefore, it was possible to observe significant relationships between macrophyte and macroinvertebrate pairs along an entire river ecosystem. It allows us to demonstrate that there are some prospective for trying to increase the knowledge on macrophytes-macroinvertebrates co-occurrence and for suggesting to use only single pair of co-occurring taxa in the monitoring running water quality. Aware that more investigations ought to be

  19. Estimating cyclopoid copepod species richness and geographical distribution (Crustacea across a large hydrographical basin: comparing between samples from water column (plankton and macrophyte stands

    Directory of Open Access Journals (Sweden)

    Gilmar Perbiche-Neves

    2014-06-01

    Full Text Available Species richness and geographical distribution of Cyclopoida freshwater copepods were analyzed along the "La Plata" River basin. Ninety-six samples were taken from 24 sampling sites, twelve sites for zooplankton in open waters and twelve sites for zooplankton within macrophyte stands, including reservoirs and lotic stretches. There were, on average, three species per sample in the plankton compared to five per sample in macrophytes. Six species were exclusive to the plankton, 10 to macrophyte stands, and 17 were common to both. Only one species was found in similar proportions in plankton and macrophytes, while five species were widely found in plankton, and thirteen in macrophytes. The distinction between species from open water zooplankton and macrophytes was supported by nonmetric multidimensional analysis. There was no distinct pattern of endemicity within the basin, and double sampling contributes to this result. This lack of sub-regional faunal differentiation is in accordance with other studies that have shown that cyclopoids generally have wide geographical distribution in the Neotropics and that some species there are cosmopolitan. This contrasts with other freshwater copepods such as Calanoida and some Harpacticoida. We conclude that sampling plankton and macrophytes together provided a more accurate estimate of the richness and geographical distribution of these organisms than sampling in either one of those zones alone.

  20. Aquatic macrophytes as indicators of heavy metal pollution of water in DTD canal system

    Directory of Open Access Journals (Sweden)

    Pajević Slobodanka P.

    2003-01-01

    Full Text Available The aim of this investigation was to establish the presence or absence of chemical contamination of water and the littoral zone (banks of Danube-Tisza-Danube (DTD canal system. The investigation covered the canal section from Bezdan to Prigrevica. By analyzing the chemical composition of dominant aquatic species in four locations of the section, we defined the species with the highest capacity to accumulate nutrients and heavy metals. Concentrations of P and K as well as of a beneficial element Na in the tissues of the analyzed macrophytes were both species- and site-dependent. The highest accumulation was registered for Ceratophyllum demersum while the species Elodea canadensis showed increased P and K accumulation values in the location Sombor. The lowest concentrations of almost all heavy metals were recorded near Sombor, indicating that this section suffered the lowest chemical pollution. Highest concentrations of all of the analyzed heavy metals were recorded in the tissue of Ceratophyllum demersum from the location Prigrevica, possibly due to the influx of polluted drainage waters from surrounding agricultural areas as well as industrial wastewaters. The obtained results showed that the enforcement of biomonitoring and analyses of other parameters indicative of ecosystem conditions might be useful for improved protection of areas experiencing a strong human impact.

  1. Ecological evaluation of transitional and coastal waters: A marine benthic macrophytes-based model

    Directory of Open Access Journals (Sweden)

    S. ORFANIDIS

    2012-12-01

    Full Text Available A model to estimate the ecological status and identify restoration targets of transitional and coastal waters was developed. Marine benthic macrophytic species (seaweeds, seagrasses were used to indicate shifts in the aquatic ecosystem from the pristine state with late-successional species (Ecological State Group I to the degraded state with opportunistic (ESG II species. The first group comprises species with a thick or calcareous thallus, low growth rates and long life cycles (perennials, whereas the second group includes sheet-like and filamentous species with high growth rates and short life cycles (annuals. Seagrasses were included in the first group, whereas Cyanophyceae and species with a coarsely branched thallus were included in the second group.The evaluation of ecological status into five categories from high to bad includes a cross comparison in a matrix of the ESGs and a numerical scoring system (Ecological Evaluation Index. The model could allow comparisons, ranking and setting of priorities at regional and national levels fulfilling the requirements of the EU Water Frame Directive. A successful application of the model was realized in selected lagoons of the Macedonian and Thrace region (North Greece and in the Saronic Gulf coastal ecosystems (Central Greece.

  2. Water hardness reduces the accumulation and toxicity of uranium in a freshwater macrophyte (Ceratophyllum demersum)

    Energy Technology Data Exchange (ETDEWEB)

    Markich, Scott J., E-mail: smarkich@optusnet.com.au

    2013-01-15

    There is a lack of good quality data and mechanistic understanding on the effects of true water hardness (calcium (Ca) and magnesium (Mg)) on the bioavailability and toxicity of uranium (U) to freshwater biota. This study determined the effect of true water hardness (20, 75, 150, 275 and 400 mg CaCO{sub 3} L{sup −1}) on the cell surface binding affinity (log K), accumulation and toxicity (growth inhibition) of U in a submerged, rootless, macrophyte (Ceratophyllum demersum) in a synthetic freshwater with constant alkalinity (13 mg CaCO{sub 3} L{sup −1}) and pH (6.2) over 7 days. A 20-fold increase in water hardness resulted in a 4-fold decrease in U toxicity (median effect concentration (EC50) = 134 μg L{sup −1} U at 20 mg CaCO{sub 3} L{sup −1} hardness, increasing to 547 μg L{sup −1} U at 400 mg CaCO{sub 3} L{sup −1} hardness), cell surface binding affinity (log K = 6.25 at 20 mg CaCO{sub 3} L{sup −1} hardness, decreasing to log K = 5.64 at 400 mg CaCO{sub 3} L{sup −1} hardness) and accumulation (the concentration factor decreased from 63 at 20 mg CaCO{sub 3} L{sup −1} hardness to 15 at 400 mg CaCO{sub 3} L{sup −1} hardness) of U. Calcium provided a 4-fold greater protective effect against U accumulation and toxicity compared to Mg. Speciation calculations indicated negligible differences in the percentages of key U species (UO{sub 2}{sup 2+}, UO{sub 2}OH{sup +}, UO{sub 2}(OH){sub 2}) over the range of water hardness tested. The inhibition of U binding at the cell surface, and subsequent uptake, by C. demersum, with increasing Ca and/or Mg concentration, may be explained in terms of (i) competition between Ca{sup 2+}/Mg{sup 2+} and UO{sub 2}{sup 2+} (and/or UO{sub 2}OH{sup +}) for physiologically active sites at the cell surface, and/or (ii) reduced negative charge (electrical potential) at the cell surface, resulting in a decrease in the activity of UO{sub 2}{sup 2+} (and/or UO{sub 2}OH{sup +}) at the plant/water interface (boundary layer

  3. Macrophyte and pH buffering updates to the Klamath River water-quality model upstream of Keno Dam, Oregon

    Science.gov (United States)

    Sullivan, Annett B.; Rounds, Stewart A.; Asbill-Case, Jessica R.; Deas, Michael L.

    2013-01-01

    A hydrodynamic, water temperature, and water-quality model of the Link River to Keno Dam reach of the upper Klamath River was updated to account for macrophytes and enhanced pH buffering from dissolved organic matter, ammonia, and orthophosphorus. Macrophytes had been observed in this reach by field personnel, so macrophyte field data were collected in summer and fall (June-October) 2011 to provide a dataset to guide the inclusion of macrophytes in the model. Three types of macrophytes were most common: pondweed (Potamogeton species), coontail (Ceratophyllum demersum), and common waterweed (Elodea canadensis). Pondweed was found throughout the Link River to Keno Dam reach in early summer with densities declining by mid-summer and fall. Coontail and common waterweed were more common in the lower reach near Keno Dam and were at highest density in summer. All species were most dense in shallow water (less than 2 meters deep) near shore. The highest estimated dry weight biomass for any sample during the study was 202 grams per square meter for coontail in August. Guided by field results, three macrophyte groups were incorporated into the CE-QUAL-W2 model for calendar years 2006-09. The CE-QUAL-W2 model code was adjusted to allow the user to initialize macrophyte populations spatially across the model grid. The default CE-QUAL-W2 model includes pH buffering by carbonates, but does not include pH buffering by organic matter, ammonia, or orthophosphorus. These three constituents, especially dissolved organic matter, are present in the upper Klamath River at concentrations that provide substantial pH buffering capacity. In this study, CE-QUAL-W2 was updated to include this enhanced buffering capacity in the simulation of pH. Acid dissociation constants for ammonium and phosphoric acid were taken from the literature. For dissolved organic matter, the number of organic acid groups and each group's acid dissociation constant (Ka) and site density (moles of sites per mole of

  4. Water hardness reduces the accumulation and toxicity of uranium in a freshwater macrophyte (Ceratophyllum demersum).

    Science.gov (United States)

    Markich, Scott J

    2013-01-15

    There is a lack of good quality data and mechanistic understanding on the effects of true water hardness (calcium (Ca) and magnesium (Mg)) on the bioavailability and toxicity of uranium (U) to freshwater biota. This study determined the effect of true water hardness (20, 75, 150, 275 and 400 mg CaCO(3) L(-1)) on the cell surface binding affinity (log K), accumulation and toxicity (growth inhibition) of U in a submerged, rootless, macrophyte (Ceratophyllum demersum) in a synthetic freshwater with constant alkalinity (13 mg CaCO(3) L(-1)) and pH (6.2) over 7 days. A 20-fold increase in water hardness resulted in a 4-fold decrease in U toxicity (median effect concentration (EC50)=134 μg L(-1)U at 20 mg CaCO(3 )L(-1) hardness, increasing to 547 μg L(-1) U at 400 mg CaCO(3) L(-1) hardness), cell surface binding affinity (log K=6.25 at 20 mg CaCO(3) L(-1) hardness, decreasing to log K=5.64 at 400 mg CaCO(3) L(-1) hardness) and accumulation (the concentration factor decreased from 63 at 20 mg CaCO(3) L(-1) hardness to 15 at 400 mg CaCO(3) L(-1) hardness) of U. Calcium provided a 4-fold greater protective effect against U accumulation and toxicity compared to Mg. Speciation calculations indicated negligible differences in the percentages of key U species (UO(2)(2+), UO(2)OH(+), UO(2)(OH)(2)) over the range of water hardness tested. The inhibition of U binding at the cell surface, and subsequent uptake, by C. demersum, with increasing Ca and/or Mg concentration, may be explained in terms of (i) competition between Ca(2+)/Mg(2+) and UO(2)(2+) (and/or UO(2)OH(+)) for physiologically active sites at the cell surface, and/or (ii) reduced negative charge (electrical potential) at the cell surface, resulting in a decrease in the activity of UO(2)(2+) (and/or UO(2)OH(+)) at the plant/water interface (boundary layer), and consequently, less U bound to physiologically active cell surface sites. In the absence of a biotic ligand model for U, the results of this study (together with

  5. Effects of taxonomy, sediment, and water column on C:N:P stoichiometry of submerged macrophytes in Yangtze floodplain shallow lakes, China.

    Science.gov (United States)

    Su, Haojie; Wu, Yao; Xie, Ping; Chen, Jun; Cao, Te; Xia, Wulai

    2016-11-01

    Carbon (C), nitrogen (N) and phosphorus (P) are the three most important essential elements limiting growth of primary producers. Submerged macrophytes generally absorb nutrients from sediments by root uptake. However, the C:N:P stoichiometric signatures of plant tissue are affected by many additional factors such as taxonomy, nutrient availability, and light availability. We first revealed the relative importance of taxonomy, sediment, and water column on plant C:N:P stoichiometry using variance partitioning based on partial redundancy analyses. Results showed that taxonomy was the most important factor in determining C:N:P stoichiometry, then the water column and finally the sediment. In this study, a significant positive relationship was found between community C concentration and macrophyte community biomass, indicating that the local low C availability in macrophytes probably was the main reason why submerged macrophytes declined in Yangtze floodplain shallow lakes. Based on our study, it is suggested that submerged macrophytes in Yangtze floodplain shallow lakes are primarily limited by low light levels rather than nutrient availability.

  6. Assessment of metallic pollution status of surface water and aquatic macrophytes of earthen dams in Ilorin, north-central of Nigeria as indicators of environmental health

    Directory of Open Access Journals (Sweden)

    Clement O. Ogunkunle

    2016-10-01

    Full Text Available The functional quality of an aquatic ecosystem is a reflection of the health of the environment. Therefore, the present study evaluates the trace metal contamination (Pb, Cd, Ni and Mn of water and aquatic macrophytes in Asa, Agba, Unilorin and Sobi (Moro earthen dams, north-central Nigeria to evaluate the level of anthropogenic impact on the immediate environment. The concentrations of trace metals in samples of water and available macrophytes from the earthen dams were determined by Atomic Absorption Spectrophotometry. Trace metal contamination of surface water in the earthen dams was assessed using metal index (MPI and metal pollution index (HPI. The biological accumulation factor of trace metals in the aquatic macrophytes was extrapolated from trace metal concentrations in the water and macrophyte samples. The results of the MPI revealed gross metal contamination of the surface water by Pb and Cd (>6.0 for both metals in the four earthen dams; while Agba and Sobi dams were slightly contaminated by Ni (MPIs = 1.43 and 1.14 respectively. All the earthen dams were considered safe from Mn contamination (MPI  100, but Asa dam (HPI = 2682.4 was the most contaminated. The biological accumulation factor of Mn in the macrophytes indicated Ceratophyllum demersum, Pycreus lanceolatus and Pistia stratiotes as moderate accumulators of Mn, and can be used as bioindicators in monitoring Mn pollution of aquatic ecosystem. The obtained results in this study showed that the earthen dams are polluted by Pb, Cd and Ni which pose human health risks to the inhabitants through drinking water.

  7. Application of hyperspectral techniques to multispectral data: spectral mixture analysis (SMA) in mapping of emergent macrophytes in a water-hyacinth-infested area

    Science.gov (United States)

    Idawo, Cuthbert; Jajah, Munzer; Laneve, Giovanni

    2004-02-01

    Water hyacinth (Eicchornia crassipes (Mart.) Solms) is an invasive aquatic macrophyte that has infested the lake Victoria, East Africa, since the late 1980s. It has been associated with major negative economic and ecological impact of this important water resource in East Africa. Remote sensing technology has significant potential in mapping this fast growing floating weed, in a mostly inaccessible area for field measurements. Our study site is the Winam Gulf, on the Kenyan part of the Lake, which has had the highest reported infestation in recent years. The paper describes a study to evaluate the ability of ETM+ multispectral imagery in mapping water hyacinth and associated macrophytes in the hyacinth infested Winam Gulf. By applying hyperspectral techniques on multispectral data, a spectral mixture analysis was undertaken using image-derived endmembers. The study was also an evaluation of an alternative way of acquiring emergent macrophytic endmembers in cases where limitations like lack of hyperspectral data, spectrometric measurements and spectral libraries exist. The results demonstrate that whereas it is possible to discriminate and map the different spectral constituents, a spectral library of the endmembers under investigation would be required for positive identification, especially for macrophytes that are closely related spectrally, fast growing, have varying concentrations (density) spatially, and are non-static in nature.

  8. Effect of repeated irrigation with water containing varying levels of total organic carbon on the persistence of Escherichia coli O157:H7 on baby spinach.

    Science.gov (United States)

    Ingram, David T; Patel, Jitu; Sharma, Manan

    2011-05-01

    The California lettuce and leafy greens industry has adopted the Leafy Greens Marketing Agreement (LGMA), which allows for 126 most-probable-number (MPN) Escherichia coli per 100 ml in irrigation water. Repeat irrigation of baby spinach plants with water containing E. coli O157:H7 and different levels of total organic carbon (TOC) was used to determine the epiphytic survival of E. coli O157:H7. Three irrigation treatments (0 ppm of TOC, 12 or 15 ppm of TOC, and 120 or 150 ppm of TOC) were prepared with bovine manure containing E. coli O157:H7 at either low (0 to 1 log CFU/100 ml) or high (5 to 6 log CFU/100 ml) populations, and sprayed onto baby spinach plants in growth chambers by using a fine-mist airbrush. MPN and direct plating techniques were used to determine the E. coli O157:H7 populations on the aerial plant tissue. Plants irrigated with high E. coli O157:H7 populations, regardless of TOC levels, showed a 3-log reduction within the first 24 h. Low levels of E. coli O157:H7 were observed for up to 16 days on all TOC treatments, ranging from 76.4 MPN per plant (day 1) to 0.40 MPN per plant (day 16). No viable cells were detected on spinach tissue 24 h after irrigation with water containing fewer than 126 CFU/100 ml E. coli O157:H7. Under growth chamber conditions in this study, E. coli O157:H7 populations in irrigation water that complies with the LGMA standards will not persist for more than 24 h when applied onto foliar surfaces of spinach plants.

  9. High-yield cultivation techniques of large-leaved water spinach(Ipomoea aquatica Forsk)%大叶空心菜高产栽培技术

    Institute of Scientific and Technical Information of China (English)

    叶敏

    2015-01-01

    总结大叶空心菜高产栽培技术,主要包括播种育苗、定植、田间管理、病虫害防治、采收等方面内容。%High-yield cultivation techniques of large-leaved water spinach was summarized,mainly including culture of see-ding,field planting,field management,control of diseases and pests,harvesting and other aspects.

  10. Spatio-temporal distribution of Diaphanosoma brachyurum (Cladocera: Sididae in freshwater reservoir ecosystems: importance of maximum water depth and macrophyte beds for avoidance of fish predation

    Directory of Open Access Journals (Sweden)

    Jong-Yun Choi

    2014-10-01

    Full Text Available In empirical studies, Cladocera is commonly utilized as a primary food source for predators such as fish, thus, predator avoidance are important strategies to sustain their population in freshwater ecosystems. In this study, we tested the hypothesis that water depth is an important factor in determining the spatial distribution of Diaphanosoma brachyurum Liévin, 1848 in response to fish predation. Quarterly monitoring was implemented at three water layers (i.e., water surface and middle and bottom layers in 21 reservoirs located in the southeastern part of South Korea. D. brachyurum individuals were frequently observed at the study sites and exhibited different spatial patterns of distribution in accordance with the maximum depth of the reservoirs. In the reservoirs with a maximum depth of more than 6 m, high densities of D. brachyurum were observed in the bottom layers; however, in the shallower reservoirs (maximum depth <6 m, D. brachyurum were concentrated in the surface layer. Moreover, during additional surveys, we observed a trend in which D. brachyurum densities increased as the maximum depth or macrophyte biomass increased. Gut contents analysis revealed that predatory fishes in each reservoir frequently consumed D. brachyurum; however, the consumption rate abruptly decreased in reservoirs where the maximum depth was more than 11 m or in the shallow reservoirs supporting a macrophyte bed. Interestingly, the reservoirs more than 11-m depth supported high densities of D. brachyurum in the bottom layer and in the surface macrophyte bed. Based on these results, reservoirs with a maximum depth of more than 11 m or those with a macrophyte bed may provide a refuge for D. brachyurum to avoid fish predation. Compared with other cladoceran species, D. brachyurum readily exploits various types of refugia (in this study, the deep layer or surface macrophyte bed, which may help explain why this species is abundant in various types of reservoirs.

  11. Effects of waste water irrigation on soil properties and soil fauna of spinach fields in a West African urban vegetable production system.

    Science.gov (United States)

    Stenchly, Kathrin; Dao, Juliane; Lompo, Désiré Jean-Pascal; Buerkert, Andreas

    2017-03-01

    The usage of inadequately processed industrial waste water (WW) can lead to strong soil alkalinity and soil salinization of agricultural fields with negative consequences on soil properties and biota. Gypsum as a soil amendment to saline-sodic soils is widely used in agricultural fields to improve their soil physical, chemical and hence biological properties. This study aimed at analysing the effects of intensive WW irrigation on the structure and composition of soil-dwelling arthropods on spinach fields (Spinacia oleracea L.) in a West African urban vegetable production system. We used gypsum as a soil amendment with the potential to alleviate soil chemical stress resulting in a potentially positive impact on soil arthropods. A total of 32 plots were established that showed a gradient in soil pH ranging from slight to strong soil alkalinity and that were irrigated with WW (n = 12) or clean water (CW; n = 20), including eight plots into which gypsum was incorporated. Our study revealed a high tolerance of soil-dwelling arthropods for alkaline soils, but spinach fields with increased soil electrical conductivity (EC) showed a reduced abundance of Hymenoptera, Diptera and Auchenorrhyncha. Arthropod abundance was positively related to a dense spinach cover that in turn was not affected by WW irrigation or soil properties. Gypsum application reduced soil pH but increased soil EC. WW irrigation and related soil pH affected arthropod composition in the investigated spinach fields which may lead to negative effects on agronomical important arthropod groups such as pollinators and predators. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Role of Cellulose and Colanic Acid in Attachment of Shiga Toxin-Producing Escherichia coli to Lettuce and Spinach in Different Water Hardness Environments.

    Science.gov (United States)

    Lee, Chi-Ching; Chen, Jinru; Frank, Joseph F

    2015-08-01

    This study investigated the role of extracellular cellulose production by Shiga toxin-producing Escherichia coli (STEC) on attachment to lettuce and spinach in different water hardness environments. Two cellulose-producing wild-type STEC strains, 19 (O5:H-) and 49 (O103:H2), and their cellulose-deficient derivatives were used. Strain 49 also produced colanic acid as a constituent of its extracellular polymeric substances. Attached cells were determined by plate counts on the surface and cut edge of the leaves after an attachment period of 2 h at 4°C. Hydrophobicity and surface charge of the cells were determined. Strain 49 attached at levels 0.3 and 0.6 log greater to the surface and 0.9 and 0.4 log greater to the cut edges of spinach compared to strain 19 for both wild-type and cellulose-deficient cells (P > 0.05). Cellulose-producing cells attached more to the surface of lettuce but not of spinach than did cellulose-deficient cells. However, more cellulose-deficient cells attached (at levels 0.66 and 0.3 log greater) to the cut edge of lettuce (representing damaged tissue) than did cellulose-proficient cells (P > 0.05). Colanic acid production was associated with cell surfaces of low hydrophobicity. There was a decreasing level of attachment for the colanic acid-producing strain when water hardness increased from 200 to 1,000 pm on lettuce and spinach leaf surfaces, but no effects were seen for other cells. This decreased attachment was associated with a more negative surface charge. Cells that produced colanic acid were less hydrophobic and exhibited greater attachment to the surface and cut edge of spinach when compared to cells that did not produce colanic acid. Attachment of colanic acid-producing cells to leafy green surfaces was enhanced in higher water hardness environments. These data indicate that attachment of E. coli O157:H7 to leafy greens involves multiple mechanisms that are influenced by the type of leafy green, damage to the leaf, and the water

  13. Change of trace elements content in sewage water under the influence of hydrophilic macrophytes

    Science.gov (United States)

    Akhmed-Ogly, K. V.; Savichev, O. G.

    2014-08-01

    According to the researches carried out by authors in 2013, the estimate of the effectiveness of domestic sewage treatment with the help of hydrophilic vegetation was received. It has been shown that if sewage is treated with the help of macrophytes, copper and lead concentration reduces. Thus, if the volume of sewage treated by reed mace is 500 ml and 1 l, lead concentration decreases 5 and 3,5 times, if sewage is treated by reed, lead concentration decreases 2,5 times in both cases; if sewage is treated by reed mace copper concentration decreases 0,9 and 1,8 times (if the volume of sewage is 500 ml and 1 l), if sewage is treated by reed, copper concentration decreases 1,4 and 1,5 times respectively. The conclusion has been drawn: in West Siberia it is possible to use the shallow reservoirs with natural aeration and hydrophilic vegetation for effective sewage treatment from such heavy metals as lead and copper.

  14. Design of an integrated piggery system with recycled water, biomass production and water purification by vermiculture, macrophyte ponds and constructed wetlands.

    Science.gov (United States)

    Morand, Philippe; Robin, Paul; Pourcher, Anne-Marie; Oudart, Didier; Fievet, Sebastien; Luth, Daniel; Cluzeau, Daniel; Picot, Bernadette; Landrain, Brigitte

    2011-01-01

    Since 2001 the swine experimental station of Guernévez has studied biological treatment plants for nutrient recovery and water recycling, suited to the fresh liquid manure coming out of flushing systems. An integrated system with continuous recycling was set up in 2007, associated with a piggery of 30 pregnant sows. It includes a screen, a vermifilter, and macrophyte ponds alternating with constructed wetlands. The screen and the vermifilter had a lower removal efficiency than in previous studies on finishing pigs. A settling tank was then added between the vermifilter and the first lagoon to collect the worm casts. A second vermifilter was added to recover this particulate organic matter. A storage lagoon was added to compensate for evaporative losses and complete pollution abatement, with goldfish as a bioindicator of water quality. The removal efficiency of the whole system was over 90% for COD and nitrogen, over 70% for phosphorus and potassium, and more than 4 logarithmic units for pathogens (E. coli, enterococci, C perfringens). Plant production was about 20 T DM ha(-1) y(-1). Floating macrophytes (Azolla caroliniana, Eichhornia crassipes, Hydrocotyle vulgaris) were more concentrated in nutrients than helophytes (Phragmites australis, Glyceria aquatica,…). Azolla caroliniana was successfully added to feed finishing pigs.

  15. 富营养水体中肥沃底质对沉水植物生长的胁迫%STRESS OF FERTILE SEDIMENT ON THE GROWTH OF SUBMERSED MACROPHYTES IN EUTROPHIC WATERS

    Institute of Scientific and Technical Information of China (English)

    倪乐意

    2001-01-01

    @@Submersed macrophytes are real water plants because they are rooted in sediment and can complete their growth and reproduction inside the overlying water. Providing a linkage between water and sediment, they have the closest interaction with water environment. This interaction is very complicate partly because sediment are not only served as a base of physical attachment. Recent attention has focused on the effects of submersed macrophytes on sediment redox, nutrient statues and the effects of sediment organic composition on macrophyte growth. This line of investigation has been thoroughly reviewed by Barko et al.(1991). Long-term ecological records of the decline of submersed macrophytes in the progress of eutrophication have suggested that sediment may play an important role for the decline due to its close relations to macrophyte growth and distribution. Therefore, more concerns should be given to the inhibitory effects of fertile sediment on submersed macrophytes when nutrients are excessive for their growth. However, this line of investigation and experiments are relatively weak up-to-date.

  16. Feasibility of Treatment of the Waters of a Wadi Charged in Iron by Filters Planted of Macrophytes (Phragmites australis

    Directory of Open Access Journals (Sweden)

    Semadi Faten

    2009-01-01

    Full Text Available Problem statement: The context is summarized by the presence of important Steel manufactory close to the wadi Meboudja and its effluents rejected into the wadi which contaminate the water used for irrigation by the local farmer. Approach: The goal is to determine the usefulness of Macrophytes (Phragmites australis to filter some specific industrial effluents (Fe, Cu, Zn, Pb, Mn and Cr present into water of wadi Meboudja. The use of Reeds (Phragmites australis can be considered as a biologic and an economic solution to minimize the concentration of some industrial effluent, especially Iron which is highlighted in this article. An experimental device was built, and considered as pilot, formed of three basins plus reservoir. We use the water of Wadi to irrigate the "Phragmites australis" which are initially appropriated from Oubaiira Lake in a natural medium. Water and Reeds samples are selected for analyses. Results: Iron is found in important concentration compared to the other Elements Metal Traces (ETM. Such a variation seems to be directly related to the rate of industrial production, fluctuations of the climatic factors and the capacities of assimilation of the plants crop. It is essentially concentrated into the roots of Reeds compared to stems and leafs. For example, in one repetition we found difference between the exit sample and the entry one in basin-1of (60-23= 37 mg g-1 into roots. Conclusion: Plantation of Reeds (Phragmites australis seems a natural solution to reduce elements metal traces, in particularly Iron, into water of wadi Meboudja. Other analysis on the garden products of local farmers should be conducted in order to quantify possible hazards on the health of consumers.

  17. Inoculating chlamydospores of Trichoderma asperellum SM-12F1 changes arsenic availability and enzyme activity in soils and improves water spinach growth.

    Science.gov (United States)

    Su, Shiming; Zeng, Xibai; Bai, Lingyu; Williams, Paul N; Wang, Yanan; Zhang, Lili; Wu, Cuixia

    2017-05-01

    Arsenic (As)-contaminated agricultural soils threaten crop yields and pose a human health risk. Augmentation of exogenous microorganisms exhibiting plant-growth promoting and As speciation changing shows potential to improve crop growth and change soil As availability. Trichoderma asperellum SM-12F1 exhibiting both traits was developed into chlamydospores to improve its persistence in contaminated soils. After inoculation, As availability and enzyme activity in two types of soils and the growth as well as As uptake of water spinach (Ipomoea aquatic Forsk.) were investigated. The results indicated that inoculation significantly improved water spinach growth in both soils. Inoculating chlamydospores at 5% significantly increased As concentration (139%), bioconcentration factor (150%), and translocation factor (150%) in water spinach grown in Chenzhou (CZ) soils, while no significant change for these in Shimen (SM) soils. Inoculating chlamydospores at 5% caused a significant increase (16%) of available As content in CZ soils, while a significant decrease (13%) in SM soils. Inoculation significantly caused As methylation in both soils, while significant As reduction merely observed in CZ soils. The differential changes in available As contents in both soils were attributed to the soil pH, As fractionations and speciation characteristics. Furthermore, Inoculating chlamydospores at 5% significantly improved the activities of β-glucosidase (155%), chitinase (211%), and phosphatase (108%) in SM soils, while significant decreases in β-glucosidase (81%), phosphatase (54%), aminopeptidase (60%), and catalase (67%) in CZ soils. Bioaugmentation and As availability change were responsible for this result. These observations will be helpful for the application of fungal chlamydospores in the future bioremediation. Copyright © 2017. Published by Elsevier Ltd.

  18. Ultrafast spectroscopy studies on the mechanism of electron transfer and energy conversion in the isolated pseudo ginseng, water hyacinth and spinach chloroplasts

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The spectroscopy characteristics and the fluorescence lifetime for the chloroplasts isolated from the pseudo ginseng, water hyacinth and spinach plant leaves have been studied by absorption spectra, low temperature steady-state fluorescence spectroscopy and single photon counting measurement under the same conditions and by the same methods. The similarity of the absorption spectra for the chloroplasts at room temperature suggests that different plants can efficiently absorb light of the same wavelength. The fluorescence decays in PS II measured at the natural QA state for the chloroplasts have been fitted by a three-exponential kinetic model. The three fluorescence lifetimes are 30, 274 and 805 ps for the pseudo ginseng chloroplast; 138, 521 and 1494 ps for the water hyacinth chloroplast; 197, 465 and 1459 ps for the spinach chloroplast, respectively. The slow lifetime fluorescence component is assigned to a collection of associated light harvesting Chl a/b proteins, the fast lifetime component to the reaction center of PS II and the middle lifetime component to the delay fluorescence of recombination of and Pheo-. The excitation energy conversion efficiency (η) in PS II RC is defined and calculated on the basis of the 20 ps electron transfer time constant model, 60%, 87% and 91% for the pseudo ginseng, water hyacinth and spinach chloroplasts, respectively. This interesting result is in unconformity with what is assumed to be 100% efficiency in PS II RC. Our result in this work stands in line with the 20 ps electron transfer time constant in PS II rather sound and the water hyacinth plant grows slower than the spinach plant does as envisaged on the efficiency. But, our results predict that those plants can perform highly efficient transfer of photo-excitation energy from the light-harvesting pigment system to the reaction center (closely to 100%). The conclusion contained in this paper reveals the plant growth characteristics expressed in the primary processes of

  19. A macrophyte submodel for aquatic ecosystems

    Science.gov (United States)

    Collins, Carol Desormeau; Wlosinski, Joseph H.

    1989-01-01

    A macrophyte submodel has been incorporated and tested in CE-QUAL-R1, a one-dimensional, vertically averaged model of reservoir water quality. A quasi two-dimensional scheme was necessary to represent the spatial relationship of macrophytes in reservoirs adequately. The macrophyte processes modeled were photosynthesis, dark respiration, excretion and nonpredatory mortality. Process equations for photosynthesis as a function of light and temperature, and dark respiration as a function of temperature were tested using data from laboratory studies. The submodel was field tested, along with other components of CE-QUAL-R1, using data collected at Eau Galle Reservoir, WI, U.S.A.

  20. Checklist of the Aquatic Macrophytes

    African Journals Online (AJOL)

    The emergent macrophytes formed 93.3% of the total macrophyte .... accounted for 2.2% while emergent species with. 93.30% of ... proliferation of floating aquatic macrophytes like ..... Geoelectric Evalution of the Groundwater Potential of Parts.

  1. Artificial regulation of water level and its effect on aquatic macrophyte distribution in Taihu Lake.

    Directory of Open Access Journals (Sweden)

    Dehua Zhao

    Full Text Available Management of water levels for flood control, water quality, and water safety purposes has become a priority for many lakes worldwide. However, the effects of water level management on the distribution and composition of aquatic vegetation has received little attention. Relevant studies have used either limited short-term or discrete long-term data and thus are either narrowly applicable or easily confounded by the effects of other environmental factors. We developed classification tree models using ground surveys combined with 52 remotely sensed images (15-30 m resolution to map the distributions of two groups of aquatic vegetation in Taihu Lake, China from 1989-2010. Type 1 vegetation included emergent, floating, and floating-leaf plants, whereas Type 2 consisted of submerged vegetation. We sought to identify both inter- and intra-annual dynamics of water level and corresponding dynamics in the aquatic vegetation. Water levels in the ten-year period from 2000-2010 were 0.06-0.21 m lower from July to September (wet season and 0.22-0.27 m higher from December to March (dry season than in the 1989-1999 period. Average intra-annual variation (CV(a decreased from 10.21% in 1989-1999 to 5.41% in 2000-2010. The areas of both Type 1 and Type 2 vegetation increased substantially in 2000-2010 relative to 1989-1999. Neither annual average water level nor CV(a influenced aquatic vegetation area, but water level from January to March had significant positive and negative correlations, respectively, with areas of Type 1 and Type 2 vegetation. Our findings revealed problems with the current management of water levels in Taihu Lake. To restore Taihu Lake to its original state of submerged vegetation dominance, water levels in the dry season should be lowered to better approximate natural conditions and reinstate the high variability (i.e., greater extremes that was present historically.

  2. EUTROPHICATION OF WATER RESERVOIRS AND ROLE OF MACROPHYTES IN THIS PROCESS

    OpenAIRE

    Joanna Jadwiga Sender; Cyprian Jaruga

    2017-01-01

    The paper presents the problem related with the process of eutrophication, with special emphasis on dam reservoirs. Eutrophication is a global process, threatening the water ecosystem on every continent. It often leads to their degradation. Particularly vulnerable to eutrophication are artificial reservoirs which are dam reservoirs. This paper describes the mechanisms of eutrophication. We also pointed to the importance of aquatic plants in the process of water purification, as well as the po...

  3. Production in aquatic macrophyte communities

    DEFF Research Database (Denmark)

    Binzer, Thomas; Sand-Jensen, Kaj

    2002-01-01

    -dimensional structure because of the strong drag and shear forces of moving water. This difference in canopy structure has been suggested to account for the three- to fivefold higher gross production rates in terrestrial than aquatic communities. To evaluate the effect of community structure in aquatic habitats, we...... to distribute photons evenly between the photosynthetic tissues. As scattering and attenuation in the water column increase, the effect of thallus structure on production declines and thin transparent macrophytes are more efficient at utilizing light than thick opaque macrophytes. The results confirm...... combined a simple mechanistic model and empirical measurements on artificially structured macroalgal communities (Ulva lactuca) with varying thallus absorptance and community density. Predicted and measured values corresponded closely and revealed that gross production in high-light environments...

  4. Differences in biofilm formation of produce and poultry Salmonella enterica isolates and their persistence on spinach plants

    Science.gov (United States)

    Repeat irrigation of spinach plants with water containing Salmonella was used to determine Salmonella persistence on spinach leaves. Spinach plants were irrigated four times (biweekly) with water containing ca. 2.1 log CFU Salmonella per 100 ml water (the maximum generic E. coli MPN recommended by...

  5. Aquatic macrophytes as indicators of heavy metal pollution of water in DTD canal system

    OpenAIRE

    Pajević Slobodanka P.; Vučković Mirjana S.; Kevrešan Žarko S.; Matavulj Milan N.; Radulović Snežana B.; Radnović Dragan V.

    2003-01-01

    The aim of this investigation was to establish the presence or absence of chemical contamination of water and the littoral zone (banks) of Danube-Tisza-Danube (DTD) canal system. The investigation covered the canal section from Bezdan to Prigrevica. By analyzing the chemical composition of dominant aquatic species in four locations of the section, we defined the species with the highest capacity to accumulate nutrients and heavy metals. Concentrations of P and K as well as of a beneficial ele...

  6. CHOICE OF MACROPHYTE SUBSTRATE IN THE USE OF DIATOMS AS INDICATORS OF POND WATER QUALITY ASSESSMENT: PRELIMINARY DATA ON THE CASE OF ALALAY POND (COCHABAMBA, BOLIVIA

    Directory of Open Access Journals (Sweden)

    Eduardo A. Morales

    2012-06-01

    Full Text Available Alalay Pond is a 230-hectare, shallow aquatic ecosystem within Cochabamba, the third largest city in Bolivia. With the aim to determine the suitability of epiphytic diatoms for water quality assessment in the pond and to choose a substrate that would hold a representative bioindicator epiphytic community, the macrophytes Schoenoplectus californicus subsp. tatora (Kunth T. Koyama, Typha dominguensis Pers., Myriophyllum verticillatum L. and Azolla filiculoides Lam. were selected and differences in diatom community composition and structure were tested. Diatoms were collected during four sampling campaigns in the March-September, 2011 period, from three stations contiguous to the pelagic zone and prepared for analysis using standard, internationally used protocols. In all, 27 samples were collected from which 28 taxa characteristic of eutrophic environments were identified and 17 others could not be assigned names from the literature. Although many of the species are shared among sampling sites, the epiphytic communities developing at each station were different in structure and composition. Although, there are marked temporal variations in community features on each of the macrophytes, Shannon-Wiener and Pielou indexes, as well as canonical correspondence analysis, showed no marked differences within a single campaign and station among the 4 macrophytes. Shifts in structure and composition are denoted less commonly by species replacement and more often by changes in percent relative abundance of dominant and rare species. Very few species are restricted to a station or seem to show strong preference for a particular substrate. Taking into account growth, structural and ecological characteristics, as well as some phenological features of the macrophytes, S. californicus subsp. tatora and T. dominguensis are the most suitable substrates for water quality assessments in the pond. As demonstrated by multivariate analyses, among-site and time

  7. Distribution of aquatic macrophytes in contrasting river systems: a critique of compositional-based assessment of water quality.

    Science.gov (United States)

    Demars, Benoît O L; Edwards, Anthony C

    2009-01-01

    A brief summary of the historical developments relating to plant distribution and aquatic macrophyte-nutrient indices provided a means of assessing the general context and validity of previous assumptions. This has particular current relevance because of the prominent use of bioindicators for defining nutrient enrichment. A survey of 161 sites distributed across two broadly contrasting groups of rivers (circum-neutral versus alkaline) recorded 110 species of aquatic macrophytes and these have been statistically analyzed to (i) rank and separate the individual effects of local environmental conditions and spatial isolation on species distribution in the two contrasting groups of sites; (ii) calculate a macrophyte index based on plant cover and species indicator values (Mean Trophic Rank, MTR); and finally (iii) investigate the implications for biomonitoring. Chemical, physical and hydrological site attributes together with spatial isolation, each explained a significant and at least partially independent influence over plant species distribution. It was extremely difficult, however, to separate the single effects of different site attributes on plant distribution. While some plant species are more restricted to certain environmental conditions, many appeared indifferent to the range of those being tested. The role played by nutrients (nitrogen (N) and phosphorus (P)) were either mostly indistinguishable from other site attributes (e.g., nitrate from conductivity) or subordinate (e.g., soluble reactive phosphorus, ammonium). It is therefore very unlikely that macrophyte species composition could provide a reliable bioindicator of the surrounding nutrient (N, P) status. The calculation of the plant index illustrated this unreliability by showing that strong correlations existed with many environmental variables, not just inorganic N and P.

  8. Aquatic Plant Control Research Program: Effects of Submersed Aquatic Macrophytes on Physical and Chemical Properties of Surrounding Water

    Science.gov (United States)

    1988-12-01

    period. Potamogeton foliosus Raf., Potamogeton pectinatus L., Heteranthera dubia (Jacquin) MacM., Najas flexilis (Willd.) Rostk. & Schmidt, and Elodea ...by Gons (1982) and in dense Elodea stands in a Colorado alpine lake by Buscemi (1958). Actual mechanisms by which these profiles occur are not...by a cover of Elodea canadensis. Oikos 9:239-245. Carpenter, S. R. 1980. Enrichment of Lake Wingra, Wisconsin, by submersed macrophyte decay. Ecology

  9. Effects of water turbulence on variations in cell ultrastructure and metabolism of amino acids in the submersed macrophyte, Elodea nuttallii (Planch.) H. St. John.

    Science.gov (United States)

    Atapaththu, K S S; Miyagi, A; Atsuzawa, K; Kaneko, Y; Kawai-Yamada, M; Asaeda, T

    2015-09-01

    The interactions between macrophytes and water movement are not yet fully understood, and the causes responsible for the metabolic and ultrastructural variations in plant cells as a consequence of turbulence are largely unknown. In the present study, growth, metabolism and ultrastructural changes were evaluated in the aquatic macrophyte Elodea nuttallii, after exposure to turbulence for 30 days. The turbulence was generated with a vertically oscillating horizontal grid. The turbulence reduced plant growth, plasmolysed leaf cells and strengthened cell walls, and plants exposed to turbulence accumulated starch granules in stem chloroplasts. The size of the starch granules increased with the magnitude of the turbulence. Using capillary electrophoresis-mass spectrometry (CE-MS), analysis of the metabolome found metabolite accumulation in response to the turbulence. Asparagine was the dominant amino acid that was concentrated in stressed plants, and organic acids such as citrate, ascorbate, oxalate and γ-amino butyric acid (GABA) also accumulated in response to turbulence. These results indicate that turbulence caused severe stress that affected plant growth, cell ultrastructure and some metabolic functions of E. nuttallii. Our findings offer insights to explain the effects of water movement on the functions of aquatic plants.

  10. Production in aquatic macrophyte communities

    DEFF Research Database (Denmark)

    Binzer, Thomas; Sand-Jensen, Kaj

    2002-01-01

    Many terrestrial plant canopies regulate spatial patterns in leaf density and leaf inclination to distribute light evenly between the photosynthetic tissue and to optimize light utilization efficiency. Sessile aquatic macrophytes, however, cannot maintain the same well-defined three......-dimensional structure because of the strong drag and shear forces of moving water. This difference in canopy structure has been suggested to account for the three- to fivefold higher gross production rates in terrestrial than aquatic communities. To evaluate the effect of community structure in aquatic habitats, we...... was markedly enhanced by a vertical orientation of thalli when absorptance and community density were both high. This result implies that aquatic macrophytes of high thallus absorptance and community density exposed to high light are limited in attaining high gross production rates because of their inability...

  11. Macrophytes: ecosystem engineers in UK urban rivers

    Science.gov (United States)

    Gibbs, H.; Gurnell, A.; Heppell, K.; Spencer, K.

    2012-04-01

    Macrophytes act as ecosystem engineers within river channels in that they have the ability to cause geomorphological and ecological change. They induce reductions in flow velocity and associated sediment accumulation, and their system of underground roots and rhizomes also reinforces the accumulated sediment reducing sediment erosion and resuspension and creating habitats. As sediments, particularly finer-grained, store contaminants including metals, this engineering means that in the specific context of urban rivers where sediments are more likely to be contaminated, macrophytes trap and hold contaminated sediments creating a potentially important sink of metals. However, depending on the ability for the macrophyte to reinforce the sediment and reduce erosion and resuspension, there is the potential for the sink to turn in to a source and metals to be released in to the overlying water. This research therefore looks at the ecosystem engineering ability of common macrophytes in UK urban rivers by looking at: (i) the effect upon flow velocity and sediment accumulation of Sparganium erectum (branched bur-reed); (ii) the sediment reinforcement ability of both S. erectum, Typha latifolia (bulrush) and Phalaris arundinacea (reed canary grass); and, (iii) the storage of metals within the sediment, overlying water and the macrophytes. Research was undertaken on the River Blackwater, an urban river in Surrey, UK which has extensive macrophyte growth. Flow velocity measurements and fine sediment depths were recorded both within and outside of dense stands of S. erectum. The uprooting resistance (as an indicator of sediment reinforcement) was measured for three species: S. erectum, T. latifolia and P. arundinacea. Additionally, some preliminary sampling was undertaken of the sediment, overlying water and the macrophytes to determine metal storage. Lower flow velocities and greater volumes of fine sediment were recorded within the stands of S. erectum as opposed to the

  12. Relationship between micro-invertebrates and macrophytes in a wetland: Laguna Iberá (Corrientes, Argentina). Implications for water quality monitoring.

    Science.gov (United States)

    Momo, F R; Casset, M A; Gantes, P; Torremorell, A M; Perelli, R M

    2006-01-01

    From November 2000 to September 2001, eight points in the Iberá lagoon were sampled bimonthly. At each point, pH, temperature and dissolved oxygen were measured and invertebrates were collected from the water around macrophytes by mean of a net of 180 mu m pore size. Four biotic indices were calculated and compared, given similar trends. The total number of taxa shows to be a good and simple indicator of water quality. Averaging indices across points we obtained a good positive correlation between indices and temperature. In this ecosystem, multi-habitat sampling must be used in order to obtain a good estimation of ecological integrity. The total number of taxa can be used for water quality assessment.

  13. Waste water phytodepuration, macrophytes and microphytes nutrient retention; Rassegna di tecniche relative alla depurazione naturale delle acque. Utilizzo di macrofite e microfite nei sistemi di depurazione

    Energy Technology Data Exchange (ETDEWEB)

    Morgana, Jose` Giancarlo; Corazzi, Giulio; Lestini, Marco; Naviglio, Lucia [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Ambiente

    1997-07-01

    The traditional systems of water depuration are often responsible of undesirable ecological problems. In fact, the oxidation of pollutants due to standard sewage treatments may induce the overproduction of nutrients. Therefore the wastewater effluents may cause the eutrofication of receiving water bodies. To avoid aftereffects on life conditions of aquatic organisms, nutrient retention based on microphytes and macrophytes abilities is often utilized after traditional sewage treatments. As a matter of fact, in many countries the phytodepuration basins or artificial wetlands have been designed to receive primary and/or secondary wastewater effluents or are used directly for depuration of wastewater of small towns and/or effluents of small industries. In this report they take into account the main biological features of the common techniques of natural water depuration.

  14. An optimisation approach for shallow lake restoration through macrophyte management

    Science.gov (United States)

    Xu, Z. H.; Yin, X. A.; Yang, Z. F.

    2014-06-01

    Lake eutrophication is a serious global environmental issue. Phytoremediation is a promising, cost-effective, and environmentally friendly technology for water quality restoration. However, besides nutrient removal, macrophytes also deeply affect the hydrologic cycle of a lake system through evapotranspiration. Changes in hydrologic cycle caused by macrophytes have a great influence on lake water quality restoration. As a result of the two opposite effects of macrophytes on water quality restoration (i.e. an increase in macrophytes can increase nutrient removal and improve water quality while also increasing evapotranspiration, reducing water volume and consequently decreasing water quality), rational macrophyte control through planting and harvest is very important. In this study, a new approach is proposed to optimise the initial planting area and monthly harvest scheme of macrophytes for water quality restoration. The month-by-month effects of macrophyte management on lake water quality are considered. Baiyangdian Lake serves as a case study, using the common reed. It was found that water quality was closest to Grade III on the Chinese water quality scale when the reed planting area was 123 km2 (40% of the lake surface area) and most reeds would be harvested at the end of June. The optimisation approach proposed in this study will be a useful reference for lake restoration.

  15. An optimisation approach for shallow lake restoration through macrophyte management

    Directory of Open Access Journals (Sweden)

    Z. H. Xu

    2014-01-01

    Full Text Available Lake eutrophication is a serious global environmental issue. Phytoremediation is a promising, cost-effective, and environmentally friendly technology for water quality restoration. However, besides nutrient removal, macrophytes also deeply affect the hydrologic cycle of lake system through evapotranspiration. Changes in hydrologic cycle caused by macrophytes have a great influence on lake water quality restoration. As a result of the two opposite effects of macrophytes on water quality restoration (i.e. an increase in macrophytes can increase nutrient removal and improve water quality while also increasing evapotranspiration, reducing water volume and consequently decreasing water quality, rational macrophyte control through planting and harvest is very important. In this study, a new approach is proposed to optimise the initial planting area and monthly harvest scheme of macrophytes for water quality restoration. The month-by-month effects of macrophyte management on lake water quality are considered. Baiyangdian Lake serves as a case study, using the common reed. It was found that water quality was closest to Grade III on the Chinese water quality scale when the reed planting area was 123 km2 (40% of the lake surface area and most reeds would be harvested at the end of June. The optimisation approach proposed in this study will be a useful reference for lake restoration.

  16. Effect of removal of free-floating macrophytes on zooplankton habitat in shallow wetland

    OpenAIRE

    Choi Jong-Yun; Jeong Kwang-Seuk; La Geung-Hwan; Joo Gea-Jae

    2014-01-01

    Submerged macrophytes improve the structural heterogeneity of microhabitats in aquatic ecosystems, often providing an important habitat for zooplankton. However, excessive development of free-floating macrophytes on the water surface can reduce the biomass of submerged macrophytes and result in a relatively simple habitat structure. We hypothesized that controlling the development of free-floating macrophytes would result in a more complex habitat structure by promoting the development of sub...

  17. Classifying aquatic macrophytes as indicators of eutrophication in European lakes

    NARCIS (Netherlands)

    Penning, W.E.; Mjelde, M.; Dudley, B.; Hellsten, S.; Hanganu, J.; Kolada, A.; van den Berg, Marcel S.; Poikane, S.; Phillips, G.; Willby, N.; Ecke, F.

    2008-01-01

    Aquatic macrophytes are one of the biological quality elements in the Water Framework Directive (WFD) for which status assessments must be defined. We tested two methods to classify macrophyte species and their response to eutrophication pressure: one based on percentiles of occurrence along a phosp

  18. Systems of water treatment based on macrophytes vegetation; Sistemi di depurazione delle acque basati sull`uso di vegetazione macrofita. I - Caratteristiche e funzionamento

    Energy Technology Data Exchange (ETDEWEB)

    Borin, Maurizio [Padua, Univ. (Italy). Dipt. di Agronomia Ambientale e Produzioni Vegetali; Marchetti, Claudio

    1997-05-01

    The use of wetland macrophytes in systems of treatment of polluted waters is an outstanding example of ecotechnology. Wetland systems are an interface between the aquatic system at risk of being polluted and the terrestrial system which is the source of pollutant substances. They are a cost-effective alternative to more conventional engineered systems to treat potential pollutant substances. They are also a part of the natural hydrological cycle and provide a ready means of making treated wastewater available for reuse. Wetland treatment systems use rooted, water-tolerant plant species and shallow, flooded or saturated soil conditions to provide various types of wastewater treatment. They are very flexible in terms of features, vegetation and performance, but the following basic types can be classified as follows: natural wetlands, constructed surface flow wetlands, constructed subsurface flow wetlands (reed beds). In this paper an outlook of the features and the functioning processes is given.

  19. Physical, chemical and microbiological aspects during the dry and rainy seasons in a pond covered by macrophyte used in aquaculture water supply

    Directory of Open Access Journals (Sweden)

    Samuel Generoso Dias

    Full Text Available AIM: The water quality of a pond covered by macrophytes and used as a water supply for aquaculture was evaluated during the dry and rainy seasons; METHODS: Six points were established for water sampling, at water inflow and outflow. Samplings were carried out monthly between June 2008 and May 2009; RESULTS: Inflow points P1, P2 and P3 in the pond had higher nutrient concentrations and high trophic rates. Moreover, capybaras in the area caused sediment suspension and an increase in fecal coliforms. There was significant difference (p < 0.05 in the concentrations of nutrients in water between the dry and rainy seasons. The outflow of water caused by rain carried the material around the pond directly into the water; CONCLUSIONS: The system studied was influenced by rain and lack of adequate management of the surrounding area. Water quality was deteriorated by increase in nutrient concentrations, fecal coliforms and reduction of dissolved oxygen in the water during the rainy season. This was due to allochthonous material from the area surrounding the pond that affected negatively the supply system.

  20. The impact of an industrial effluent on the water quality, submersed macrophytes and benthic macroinvertebrates in a dammed river of Central Spain.

    Science.gov (United States)

    Gonzalo, Cristina; Camargo, Julio A

    2013-10-01

    This research was conducted in the middle Duratón River (Central Spain), in the vicinity of Burgomillodo Reservoir. An industrial effluent enters the river 300 m downstream from the dam. Fluoride and turbidity levels significantly increased downstream from the effluent, these levels being to some extent affected by differential water releases from the dam. The community of submersed macrophytes exhibited slighter responses and, accordingly, lower discriminatory power than the community of benthic macroinvertebrates, this indicating that metrics and indices based on macroinvertebrates may be more suitable for the biological monitoring of water pollution and habitat degradation in dammed rivers receiving industrial effluents. However, in relation to fluoride bioaccumulation at the organism level, macrophytes (Fontinalis antipyretica and Potamogeton pectinatus) were as suitable bioindicators of fluoride pollution as macroinvertebrates (Ancylus fluviatilis and Pacifastacus leniusculus). Fluoride bioaccumulation in both hard and soft tissues of these aquatic organisms could be used as suitable bioindicator of fluoride pollution (even lower than 1 mg F(-)L(-1)) in freshwater ecosystems. Echinogammarus calvus exhibited a great sensitivity to the toxicity of fluoride ions, with a 96 h LC₅₀ of 7.5 mg F(-)L(-1) and an estimated safe concentration of 0.56 mg F(-)L(-1). The great capacity of E. calvus to take up and retain fluoride during exposures to fluoride ions would be a major cause of its great sensitivity to fluoride toxicity. It is concluded that the observed fluoride pollution might be partly responsible for the absence of this native amphipod downstream from the industrial effluent.

  1. Effect of Liquid Organic Carbon Fertilizer on Water Spinach Quality%液态有机碳肥对蕹菜品质的影响

    Institute of Scientific and Technical Information of China (English)

    陈秀莲; 魏晓琼; 吴德淼

    2014-01-01

    The influence of liquid organic carbon fertilizer on water spinach yield, economic characters and benefits was carried out in the present study. Results showed that the yield and economic benefits of the conventional fertilization and liquid organic carbon fertilizer were the highest, which increased by 8.6% and 9.6%, respectively, than conventional fertilizer and the same volume of water, the economic benefits of whichincreased by 573.2 and 639.6 yuan per 667m2, respectively.%本文探讨了液态有机碳肥对蕹菜产量、植株性状和经济效益的影响。结果表明:常规施肥+液态有机碳肥处理比常规施肥+等量清水和常规施肥分别增产8.6%和9.6%,每667m2分别增收573.2元和639.6元,液态有机碳肥对雍菜增产增收效果明显,雍菜品质较好,在农业生产上值得推广使用。

  2. Protective effect of chlorophyllin and lycopene from water spinach extract on cytotoxicity and oxidative stress induced by heavy metals in human hepatoma cells.

    Science.gov (United States)

    Yang, Ui-Jeong; Park, Tae-Sik; Shim, Soon-Mi

    2013-01-01

    The purpose of this study was to examine the inhibitory effects of ethanol extract of water spinach (EEWS) containing chlorophyll and lycopene on cytotoxicity and oxidative stress in liver induced by heavy metals. The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT assay and dichlorofluorescein (DCF) assay were conducted to measure cytotoxicity and inhibition of reactive oxygen species (ROS), respectively. Cytotoxicity was prevented at a concentration of 11.7 mg/L of EEWS. Both sodium copper chlorophyllin (SCC) and lycopene in EEWS were identified by ultraperformance liquid chromatography-photodiode array-electrospray ionization-mass spectroscopy (UPLC-PDA-ESI-MS/MSn) as major components at m/z 722.64 and 535.45, respectively. The concentrations of SCC and lycopene were 0.12 and 0.04 mg from 100 g of dried powder, respectively. Approximately 99% cytotoxicity induced by Cd was inhibited by EEWS. However, the inhibitory effect attributed to generation of ROS was similar with SCC, lycopene, and EEWS. Our results indicated that EEWS was effective in reducing cytotoxicity and oxidative stress produced by heavy metals in a HepG2 cell. Data suggest that the possible mechanism underlying the preventive action of SCC might be associated with diminished absorption of metal ions by chelating and blocking metal-mediated generation of ROS, while lycopene effects may be attributed to its high number of conjugated dienes that act as most potent singlet oxygen quenchers.

  3. The accumulation and distribution of metals in water, sediment, aquatic macrophytes and fishes of the Gruža Reservoir, Serbia.

    Science.gov (United States)

    Milošković, Aleksandra; Branković, Snežana; Simić, Vladica; Kovačević, Simona; Ćirković, Miroslav; Manojlović, Dragan

    2013-05-01

    The concentrations of iron, lead, cadmium, copper, manganese, mercury and arsenic were measured in water, sediment, five macrophytes (Typha angustifolia, Iris pseudacorus, Polygonum amphybium, Myriophyllum spicatum and Lemna gibba) and five fish species (Sander lucioperca, Abramis brama, Carassius gibelio, Silurus glanis and Arystichtys nobilis) in the Gruža Reservoir, used for water supply and recreational fishing. The concentrations of all examined elements were higher in sediment than in water. The values of the ratio between element concentrations in the sediment and those in the water were the highest for Fe and As. Among the five plant species, the highest concentrations of Pb and Mn were observed in T. angustifolia, while the highest concentrations of Fe, Cu and Hg were in L. gibba. I. pseudacorus and P. amphybium had the highest concentrations of Cd and As, respectively. Among the fish species, C. gibelio showed the highest tendency of element accumulation (Fe, Cd, Cu), followed by S. lucioperca (Pb, Hg), A. brama (Mn) and A. nobilis (As). The average concentrations of elements in fish muscle, except for As in A. nobilis (2.635 ± 0.241 mg kg(-1) ww), were below the limits that are considered safe for human consumption in accordance with the European Commission Regulation and Official Gazette of Serbia.

  4. Effect of removal of free-floating macrophytes on zooplankton habitat in shallow wetland

    Directory of Open Access Journals (Sweden)

    Choi Jong-Yun

    2014-01-01

    Full Text Available Submerged macrophytes improve the structural heterogeneity of microhabitats in aquatic ecosystems, often providing an important habitat for zooplankton. However, excessive development of free-floating macrophytes on the water surface can reduce the biomass of submerged macrophytes and result in a relatively simple habitat structure. We hypothesized that controlling the development of free-floating macrophytes would result in a more complex habitat structure by promoting the development of submerged macrophytes. After applying three experimental treatments (NR, no removal; IR, intermediate removal; CR, complete removal of free-floating macrophytes, we found that CR of free-floating macrophytes improved the growth and development of submerged macrophytes and supported a large zooplankton assemblage. However, the largest zooplankton assemblage (in terms of abundance and diversity was recorded after the IR treatment. Although submerged macrophytes were abundant in the CR treatment, the number, abundance, and density of zooplankton species were much lower than those in the IR treatment. Preferential selection of different macrophyte types by zooplankton presumably led to variation in plant utilization of niches, and the simultaneous presence of different macrophyte life forms created a complex microhabitat structure that induced high species diversity and zooplankton density.

  5. Influence of water level fluctuation on the mortality and aboveground biomass of the aquatic macrophyte Eleocharis interstincta (VAHL roemer et schults

    Directory of Open Access Journals (Sweden)

    Santos Anderson Medeiros dos

    2004-01-01

    Full Text Available The goal of this study was to study the biometric alterations of Eleocharis interstincta in response to water level fluctuations in Cabiúnas Lagoon, located on the northern coast of the state of Rio de Janeiro, in the municipality of Macaé. Three quadrats of 0.0625 m² were harvested every two weeks from June/1997 to June/1998; samples were separated into stems, dead stems (detritus and rhizome; lenghted, dried and weighted. The water level fluctuated seasonally in the macrophyte stand with two periods of drawdown. The first period occurred naturally at the end of winter and beginning of spring, when rainfall in the area was normally lowest. The second period of drawdown was the result of an artificial breaching of the sandbar that isolate the lagoon from the sea. The breach was made in the summer, at the time of highest rainfall, when the water level in the lagoon reached the maximum value recorded during the study (1.35 m. There was a strongly positive correlation of the water level with stems mean height and aboveground biomass, indicating that water level played an important role in the determination of these parameters. There was a significant difference between stem height (ANOVA; p < 0.001 and biomass (ANOVA; p < 0.001 in each sampling period, ranging from 143.9 cm and 338.8 g dry wt.m-2, before the sandbar opening, to 16.3 cm and 20.2 g dry wt.m-2 respectively after the sandbar breaching. The drastic variation of the water level, leading mass mortality of the stems, together with the lowest mean biomass/stem (0.057 g dry wt.individual-1, recorded after the sandbar breaching, did not represent a strong disturbance for E. interstincta, since the resilience time estimated for this population was about 30 days.

  6. Thermal inactivation kinetics of hepatitis A virus in spinach.

    Science.gov (United States)

    Bozkurt, Hayriye; Ye, Xiaofei; Harte, Federico; D'Souza, Doris H; Davidson, P Michael

    2015-01-16

    Leafy vegetables have been recognized as important vehicles for the transmission of foodborne viral pathogens. To control hepatitis A viral foodborne illness outbreaks associated with mildly heated (e.g., blanched) leafy vegetables such as spinach, generation of adequate thermal processes is important both for consumers and the food industry. Therefore, the objectives of this study were to determine the thermal inactivation behavior of hepatitis A virus (HAV) in spinach, and provide insights on HAV inactivation in spinach for future studies and industrial applications. The D-values calculated from the first-order model (50-72 °C) ranged from 34.40 ± 4.08 to 0.91 ± 0.12 min with a z-value of 13.92 ± 0.87 °C. The calculated activation energy value was 162 ± 11 kJ/mol. Using the information generated in the present study and the thermal parameters of industrial blanching conditions for spinach as a basis (100 °C for 120-180 s), the blanching of spinach in water at 100 °C for 120-180 s under atmospheric conditions will provide greater than 6 log reduction of HAV. The results of this study may be useful to the frozen food industry in designing blanching conditions for spinach to inactivate or control hepatitis A virus outbreaks. Copyright © 2014. Published by Elsevier B.V.

  7. Water level fluctuations in a tropical reservoir: the impact of sediment drying, aquatic macrophyte dieback, and oxygen availability on phosphorus mobilization.

    Science.gov (United States)

    Keitel, Jonas; Zak, Dominik; Hupfer, Michael

    2016-04-01

    Reservoirs in semi-arid areas are subject to water level fluctuations (WLF) that alter biogeochemical processes in the sediment. We hypothesized that wet-dry cycles may cause internal eutrophication in such systems when they affect densely vegetated shallow areas. To assess the impact of WLF on phosphorus (P) mobilization and benthic P cycling of iron-rich sediments, we tested the effects of (i) sediment drying and rewetting, (ii) the impact of organic matter availability in the form of dried Brazilian Waterweed (Egeria densa), and (iii) alternating redox conditions in the surface water. In principle, drying led to increased P release after rewetting both in plant-free and in plant-amended sediments. Highest P mobilization was recorded in plant amendments under oxygen-free conditions. After re-establishment of aerobic conditions, P concentrations in surface water decreased substantially owing to P retention by sediments. In desiccated and re-inundated sediments, P retention decreased by up to 30% compared to constantly inundated sediments. We showed that WLF may trigger biochemical interactions conducive to anaerobic P release. Thereby, E. densa showed high P release and even P uptake that was redox-controlled and superimposed sedimentary P cycling. Macrophytes play an important role in the uptake of P from the water but may be also a significant source of P in wet-dry cycles. We estimated a potential for the abrupt release of soluble reactive phosphorus (SRP) by E. densa of 0.09-0.13 g SRP per m(2) after each wet-dry cycle. Released SRP may exceed critical P limits for eutrophication, provoking usage restrictions. Our results have implications for management of reservoirs in semi-arid regions affected by WLF.

  8. 鱼-鸭-菜生态种养模式的方法与效益%Methods and Benefit of Fish-duck-water spinach Eco-cultivating and Breeding Model

    Institute of Scientific and Technical Information of China (English)

    肖建中; 吕耀平

    2011-01-01

    The fish-duck-water spinach co-cultivation system is an ecological fishery mode with high benefit. In co-cultivation system, the fish pond provides a clean activity place for the ducks, meanwhile the duck supplies an adequate source of manure for the fish; And the water spinach not only make the water clean and increases the dissolved oxygen in pond, but also prettifies it. Two treatments, fish-duck-water spinach co-cultivation model (0.53 hm2) and only breeding fish (0.53 hm2), were set in a duck farm in Shuiguan village, Chengguang town, Liandu district, Lishui county, Zhejiang province in 2010 to analyze the benefit of co-cultivation model. The pure output value of the co-cultivation pond and the control pond was 61 480 and 11 130 RMB respectively. The ratio of invest to output of them was 1.00:1.46 and 1.00:1.26 respectively. The results showed that the economic effect of the fish-duck-water spinach co-cultivation system was remarkable.%鱼、鸭、菜混养技术是一种效益较高的生态渔业模式.于2010年在浙江省丽水市莲都区城关镇水东村养鸭场进行了鱼、鸭、菜共育试验.结果表明,鱼池为鸭提供了清洁的活动场所,而鸭子排泄的粪便为养鱼提供了充足的肥源,水面的空心菜使水体清洁,提高溶氧,也使池塘美观;试验面积1.06 hm2,试验塘(面积为0.53 hm2)和对照塘(面积为0.53 hm2)净产值分别为61 480元和11 130元,投入产出比分别为1.00∶1.46和1.00∶1.26,鱼、鸭、菜混养的平均纯收入是单纯养鱼的5.5倍,经济效益显著.

  9. 菠菜与螺旋藻中水溶性蛋白抗氧化性的比较研究%Comparative Study on Antioxidant of Water-soluble Proteins from Spinach and Spirulina

    Institute of Scientific and Technical Information of China (English)

    闫岩; 傅红; 杨琳; 张新明

    2012-01-01

    对提取自菠菜和螺旋藻中水溶性蛋白的抗氧化活性进行研究.菠菜和螺旋藻蛋白的总抗氧化能力分别为160.38 U/mg和8.12 U/mg.菠菜蛋白对DPPH、羟基自由基和超氧阴离子自由基清除活性IC50分别为76.89,70.15和90.80 μg/mL,它们的抗氧化活性均高于螺旋藻中的水溶性蛋白.本试验结果表明,蛋白质的抗氧化活性源于其结合的多酚和黄酮等活性物质,且在一定浓度范围其抗氧化性与结合活性物质的量相关.%The antioxidant activities of water-soluble proteins from spinach and spirulina were studied. T-AOC of proteins from spinach and spirulina were 160.38 U/mg and 8.12 U/mg respectively. IC50 of water-soluble protein from spinach on scavenging DPPH radical, hydroxyl radical and superoxide anion radical were 76.89 μg/mL, 70.15 μg/mL and 90.80 μg/mL, respectively, which were higher than water-soluble protein from spirulina. The result showed that bioactive compounds, such as polyphenol and flavonol, combined with the protein and showed dose -effect relationship with antioxidant activities in a certain range.

  10. Biodegradable PLA (polylactic acid) hinged trays keep quality of fresh-cut and cooked spinach.

    Science.gov (United States)

    Botondi, Rinaldo; Bartoloni, Serena; Baccelloni, Simone; Mencarelli, Fabio

    2015-09-01

    This work examines the effects of packaging using two different polymeric trays with hinged lids, polyethylene terephthalate (PET) and polylactic acid (PLA), on fresh-cut and cooked spinach (Spinacia oleracea). Samples were stored in a cold room for 16 days at 4 °C. Chemical (total pigments, total polyphenols, ascorbic acid, antioxidant activity), physical (water activity), technological (colour evaluation), sensorial (aroma, visual appearance and water accumulation) and microbial (total aerobic mesophilic and psychrotrophic counts) parameters were tested. Both polymeric trays maintained the overall quality of fresh spinach for 6 days but spinach stored in PLA trays maintained its flavour longer. A significant increase in total polyphenols, antiradical activity, total carotenoids as well as a decrease in ascorbic acid in fresh spinach was observed in the first 3 days of storage in both samples. Unfortunately, the PLA package accumulated condensed water. The total microbial load of fresh-cut spinach reached about 6.3-7.3 log CFU g(-1) within 8 days. Cooked spinach packed in PLA and PET polymeric hinged trays showed the same behaviour as fresh spinach in terms of quality and shelf life. In conclusion, PLA plastic hinged trays can be used for packaging fresh-cut and cooked cut spinach, but the problem of condensed water must be solved.

  11. Spinach Effects (Amaranthus hybridus on Spatial Memory

    Directory of Open Access Journals (Sweden)

    Intan Leonita

    2015-03-01

    Full Text Available Background: Spatial memory reduction in elderly is predicted to increase up to twice every 20 years. Spinach (Amaranthus hybridus is widely consumed by Indonesian people and is believed to prevent declined spatial memory function. The aim of this study was to determine the effects of spinach on spatial memory in wistar rat induced by diazepam Methods: An experimental study was conducted during the period of October to November 2012 in Pharmacology and Therapy Laboratory, Faculty of Medicine, Universitas Padjadjaran. Twenty five wistar rats were divided into 5 groups; two groups as controls, and 3 groups were given 100, 200, and 400mg/kg BW ethanolic extract of spinach (EESL, respectively. On day 7, group 3, 4, and 5 were given 1 mg/kg BW diazepam injection. Morris water maze tests and calculations of escape latency time (ELT were performed on day 7 and 8. Data were analyzed using analysis of variance (ANOVA and least significance difference (LSD test. Results: On day 7, group 2 experienced acceleration in ELT compared to group 4 and group 5. On day 8, group 2 experienced acceleration in ELT compared to group 3 and group 4. There was no significant increase in spatial memory in group 5 (EESL 400mg/kg BW that due to the use of higher dosage does not always show better results. Conclusions: EESL can prevent impairment of spatial memory with an effective dose of 200 mg/kg BW.

  12. First steps in the Central-Baltic intercalibration exercise on lake macrophytes: where do we start?

    NARCIS (Netherlands)

    Tóth, L.G.; Poikane, S.; Penning, W.E.; Free, G.; Mäemets, H.; Kolada, A.; Hanganu, J.

    2008-01-01

    The Water Framework Directive (WFD 2000) defines macrophytes as one of the biological groups required for the ecological assessment of European surface waters. Several indices for macrophyte assessment have been proposed or are currently in use by different European states. As a first step towards p

  13. Invasive crayfish threaten the development of submerged macrophytes in lake restoration.

    Directory of Open Access Journals (Sweden)

    Jessica E M van der Wal

    Full Text Available Submerged macrophytes enhance water transparency and aquatic biodiversity in shallow water ecosystems. Therefore, the return of submerged macrophytes is the target of many lake restoration projects. However, at present, north-western European aquatic ecosystems are increasingly invaded by omnivorous exotic crayfish. We hypothesize that invasive crayfish pose a novel constraint on the regeneration of submerged macrophytes in restored lakes and may jeopardize restoration efforts. We experimentally investigated whether the invasive crayfish (Procambarus clarkii Girard affects submerged macrophyte development in a Dutch peat lake where these crayfish are expanding rapidly. Seemingly favourable abiotic conditions for macrophyte growth existed in two 0.5 ha lake enclosures, which provided shelter and reduced turbidity, and in one lake enclosure iron was added to reduce internal nutrient loading, but macrophytes did not emerge. We transplanted three submerged macrophyte species in a full factorial exclosure experiment, where we separated the effect of crayfish from large vertebrates using different mesh sizes combined with a caging treatment stocked with crayfish only. The three transplanted macrophytes grew rapidly when protected from grazing in both lake enclosures, demonstrating that abiotic conditions for growth were suitable. Crayfish strongly reduced biomass and survival of all three macrophyte species while waterfowl and fish had no additive effects. Gut contents showed that crayfish were mostly carnivorous, but also consumed macrophytes. We show that P. clarkii strongly inhibit macrophyte development once favourable abiotic conditions for macrophyte growth are restored. Therefore, expansion of invasive crayfish poses a novel threat to the restoration of shallow water bodies in north-western Europe. Prevention of introduction and spread of crayfish is urgent, as management of invasive crayfish populations is very difficult.

  14. Restoring macrophyte diversity in shallow temperate lakes: biotic versus abiotic constraints

    NARCIS (Netherlands)

    Bakker, E.S.; Sarneel, J.M.; Gulati, R.D.; Liu, Z.; Van Donk, E.

    2013-01-01

    Although many lake restoration projects have led to decreased nutrient loads and increased water transparency, the establishment or expansion of macrophytes does not immediately follow the improved abiotic conditions and it is often unclear whether vegetation with high macrophyte diversity will retu

  15. Macrophyte-phytoplankton interactions: the relative importance of allelopathy versus other factors

    NARCIS (Netherlands)

    Mulderij, G.; Nes, van E.H.; Donk, van E.

    2007-01-01

    Submerged aquatic macrophytes are important in shallow aquatic ecosystems because they stabilize the macrophyte-dominated state by increasing water transparency in various ways. One of these is the excretion of allelopathic substances inhibitory to phytoplankton, but it is still controversial whethe

  16. Macrophyte-phytoplankton interactions: the relative importance of allelopathy versus other factors

    NARCIS (Netherlands)

    Mulderij, G.; van Nes, E.H.; Van Donk, E.

    2007-01-01

    Abstract Submerged aquatic macrophytes are important in shallow aquatic ecosystems because they stabilize the macrophyte-dominated state by increasing water transparency in various ways. One of these is the excretion of allelopathic substances inhibitory to phytoplankton, but it is still controversi

  17. Macrophyte-phytoplankton interactions: the relative importance of allelopathy versus other factors

    NARCIS (Netherlands)

    Mulderij, G.; van Nes, E.H.; Van Donk, E.

    2007-01-01

    Abstract Submerged aquatic macrophytes are important in shallow aquatic ecosystems because they stabilize the macrophyte-dominated state by increasing water transparency in various ways. One of these is the excretion of allelopathic substances inhibitory to phytoplankton, but it is still

  18. Macrophyte-phytoplankton interactions: the relative importance of allelopathy versus other factors

    NARCIS (Netherlands)

    Mulderij, G.; Nes, van E.H.; Donk, van E.

    2007-01-01

    Submerged aquatic macrophytes are important in shallow aquatic ecosystems because they stabilize the macrophyte-dominated state by increasing water transparency in various ways. One of these is the excretion of allelopathic substances inhibitory to phytoplankton, but it is still controversial

  19. Saline gas-produced waters treatment with macrophytes in a hydroponic system. Final report, July 1, 1991-December 31, 1992

    Energy Technology Data Exchange (ETDEWEB)

    Jewell, W.J.; Albright, L.D.; Cummings, R.J.; Hicks, E.E.; Nock, T.D.

    1993-06-01

    A review of potential pollution problems of natural gas produced waters emphasized that present regulations do not adversely affect gas production, but future changes may be a concern (Fillo et al.). Also, the economics of the present treatment and disposal practices are becoming unacceptably high. Thus, new and more effective methods of treating and disposing of produced waters should be sought. A hydroponic system has been developed at Cornell University and piloted on domestic sewage partly with support from the Gas Research Institute, the U.S. Department of Energy (SERI), USEPA and New York State Energy Research and Development Authority (Jewell et al.). The hydroponic plant system is referred to as the 'Nutrient Film Technique' (NFT). The general goal of the project is to conduct a preliminary study to determine the feasibility of developing a biological treatment system capable of removing organic and inorganic pollutants from highly saline waters while minimizing the effluent volume by enhanced evapotranspiration.

  20. A meta-analysis of water quality and aquatic macrophyte responses in 18 lakes treated with lanthanum modified bentonite (PHOSLOCK®)

    NARCIS (Netherlands)

    Spears, B.M.; Mackay, E.; Yasseri, S.; Gunn, I.D.M.; Waters, K.E.; Andrews, C.; Cole, S.; Ville, de M.; Kelly, M.; Meis, S.; Moore, A.L.; Nurnberg, G.K.; Oosterhout, van Frank; Lurling, M.F.L.L.W.

    2016-01-01

    Lanthanum (La) modified bentonite is being increasingly used as a geo-engineering tool for the control of phosphorus (P) release from lake bed sediments to overlying waters. However, little is known about its effectiveness in controlling P across a wide range of lake conditions or of its potential t

  1. Impacts of Different Water Pollution Sources on Antioxidant Defense Ability in Three Aquatic Macrophytes in Assiut Province, Egypt

    Directory of Open Access Journals (Sweden)

    Mohamed A.A. Gadallah

    2014-08-01

    Full Text Available The present study was undertaken to evaluate the impacts of surface water pollution with wastes coming from sewage effluents (Site 2, agricultural runoff (Site 4 and oils and detergents factory (Site 3 on the stability of leaf membrane (measured as injury %, hydrogen peroxide (H2O2, ascorbic acid (Asc A, lipid peroxidation, chlorophyll (Chl content, soluble sugars (SS, soluble proteins (SP and total free amino acids (TAA of Cyperus alopeucroides, Persicaria salicifolia and Echinochloa stagnina. Concentration of H2O2, MDA and TAA were higher in the three plants collected from polluted sites as compared with those of plants grown in control Nile site (Site1. The opposite was true for Asc A, SS and SP where their concentrations reduced significantly in response to water pollution. Leaf membrane was more damaged (high injury % in plants exposed to wastes from different sources than in plants growing at control site. The results of this study indicated that water pollution reduced the oxidative defense abilities in the three plants through reduction of Asc A activities, enhancement of H2O2 production and increasing MDA accumulation. In addition it impaired the metabolic activity through lowering the SS and SP contents and enhancement of TAA accumulation and increase membrane injury. The over production of hydrogen peroxide by the studied aquatic plants under water pollution could be used as an oxygen source needed to oxidize the more resistant organic and inorganic pollutants and used for pollution control and municipal and industrial wastewater treatment.

  2. A meta-analysis of water quality and aquatic macrophyte responses in 18 lakes treated with lanthanum modified bentonite (PHOSLOCK®)

    NARCIS (Netherlands)

    Spears, B.M.; Mackay, E.; Yasseri, S.; Gunn, I.D.M.; Waters, K.E.; Andrews, C.; Cole, S.; Ville, de M.; Kelly, M.; Meis, S.; Moore, A.L.; Nurnberg, G.K.; Oosterhout, van Frank; Lurling, M.F.L.L.W.

    2016-01-01

    Lanthanum (La) modified bentonite is being increasingly used as a geo-engineering tool for the control of phosphorus (P) release from lake bed sediments to overlying waters. However, little is known about its effectiveness in controlling P across a wide range of lake conditions or of its potential t

  3. Effects of recycled FGD liner material on water quality and macrophytes of constructed wetlands: A mesocosm experiment

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, C.; Mitsch, W.J.; Wolfe, W.E.

    2001-07-01

    This paper investigates the use of flue gas desulfurization (FGD) by-products from power plant wet scrubbers as liners in wetlands constructed to improve water quality. Mesocosm experiments were conducted over two consecutive growing seasons with different phosphorus loadings. Wetland mesocosms using FGD liners retained more total and soluble reactive phosphorus, with lower concentrations in the leachate (first year) and higher concentrations in the surface water (second year). Leachate was higher in conductivity (second year) and pH (both years) in lined mesocosms. Surface outflow did not reveal any significant difference in physicochemical characteristics between lined and unlined mesocosms. There was no significant difference in total biomass production of wetland plants between lined and unlined mesocosms.

  4. Impact of water composition on association of Ag and CeO₂ nanoparticles with aquatic macrophyte Elodea canadensis.

    Science.gov (United States)

    Van Koetsem, Frederik; Xiao, Yi; Luo, Zhuanxi; Du Laing, Gijs

    2016-03-01

    In this study, the potential association of (citrate-stabilized) Ag (14.1 ± 1.0 nm) and CeO2 (6.7 ± 1.2 nm) engineered nanoparticles (ENPs), or their ionic counterparts, with the submerged aquatic plant Elodea canadensis, was examined and, in particular, parameters affecting the distribution of the nanoparticles (or metal ions) between plant biomass and the water phase were assessed using five distinct aqueous matrices (i.e. tap water, 10 % Hoagland's solution and three natural surface water samples). Individual plants were exposed to varying concentrations of Ag and CeO2 ENPs or Ag(+) and Ce(3+) ions during 72-h-lasting batch experiments. A dose-dependent increase of silver or cerium in plant biomass was observed for both the nanoparticles and the ions, whereby exposure to the latter systematically resulted in significantly higher biomass concentrations. Furthermore, the apparent plant uptake of CeO2 ENPs appeared to be higher than that for Ag ENPs when comparing similar exposure concentrations. These findings suggest that association with E. canadensis might be affected by particle characteristics such as size, composition, surface charge or surface coating. Moreover, the stability of the ENPs or ions in suspension/solution may be another important aspect affecting plant exposure and uptake. The association of the nanoparticles or ions with E. canadensis was affected by the physicochemical characteristics of the water sample. The silver biomass concentration was found to correlate significantly with the electrical conductivity (EC), dry residue (DR) and Cl(-), K, Na and Mg content in the case of Ag ENPs or with the EC, inorganic carbon (IC) and Cl(-), NO3 (-), Na and Mg content in the case of Ag(+) ions, whereas significant relationships between the cerium biomass concentration and the EC, DR, IC and Ca content or the pH, EC, DR, IC and Cl(-), Ca and Mg content were obtained for CeO2 ENPs or Ce(3+) ions, respectively. Results also indicated that the Ag

  5. Effects of recycled FGD liner material on water quality and macrophytes of constructed wetlands: a mesocosm experiment.

    Science.gov (United States)

    Ahn, C; Mitsch, W J; Wolfe, W E

    2001-03-01

    We investigated the use of flue-gas-desulfurization (FGD) by-products from electric power plant wet scrubbers as liners in wetlands constructed to improve water quality. Mesocosm experiments were conducted over two consecutive growing seasons with different phosphorus loadings. Wetland mesocosms using FGD liners retained more total and soluble reactive phosphorus, with lower concentrations in the leachate (first year) and higher concentrations in the surface water (second year). Leachate was higher in conductivity (second year) and pH (both years) in lined mesocosms. Surface outflow did not reveal any significant difference in physicochemical characteristics between lined and unlined mesocosms. There was no significant difference in total biomass production of wetland plants between lined and unlined mesocosms although lower average stem lengths and fewer stems bearing flowers were observed in mesocosms with FGD liners. Potentially phytotoxic boron was significantly higher in the belowground biomass of plants grown in lined mesocosms with low phosphorus loading. A larger-scale, long-term wetland experiment close to full scale is recommended from this two-year mesocosm study to better predict the potentially positive and negative effects of using FGD by-products in constructed wetlands.

  6. Fluoride remediation using floating macrophytes

    Directory of Open Access Journals (Sweden)

    Naba Kumar Mondal

    2014-04-01

    Full Text Available Six aquatic macrophytes, such as Pistia stratiotes, Ceratophyllum demersum, Nymphoides indica, Lemna major, Azolla pinnata,and Eichhornia crassipes were considered for remove fluoride from aqueous solution. Five different concentrations (10, 30, 50, and 100 ppm of fluoride solution were taken in 1 L plastic container. Fixed weight (20 g of macrophytes along with 500 ml fluoride solution was taken in each plastic container for 72 hours observation. Results demonstrated all the macrophytes show highest fluoride removal during 24 h to 48 h, but after 72 h their efficiency reduced drastically. The species N. indica showed better removal efficiency than other experimental macrophytes. In general, pigment measurement data indicated higher concentration at 72 h. However, Pistia sp. showed higher concentration of pigmentation at intermediate time interval (48 h. Higher level of dry weight to fresh weight ratio was recorded for L. major and A. pinnata at all concentrations, excepting at 10 ppm. In addition, all macrophytes showed lower RGR at higher concentration. Isotherm study indicated that macrophyte C. demersum is a good fitted with Freundlich and Langmuir isotherm whereas L. major with Langmuir isotherm during 24 hours.

  7. Looking for a substituent of spinach (Spinacia oleracea) chloroplasts

    Science.gov (United States)

    Chang, Ying Ping; Yeoh, Loo Yew; Chee, Swee Yong; Lim, Tuck Meng

    2017-04-01

    Spinach's chloroplasts electron transport features are often adapted to build biofuel cells or biosensors for environment conservation. This approach may raise food security issues. The present study aimed to test on in vitro functional activity of chloroplasts from selected underutilized leaves of: Pandan (Pandanus amaryllifolius), oil palm (Elaeis guineensis) and water lettuce (Pistia stratiotes) in comparison with spinach (Spinacia oleracea). The leaves' electrical conductivity was measured to evaluate the initial cell permeability. We applied Hill's reaction to determine the photoreduction capacity of the chloroplasts. Initial electrical conductivity of leaves ranged from 11.5 to 18.5 µs/cm/g followed the order of water lettucequality marker for the leaves' chloroplasts. Chloroplasts of oil palm frond and water lettuce showed low photoreduction rate of 14 to 22%. On the other hand, the chloroplasts of both spinach and pandan leaves exerted an initial photoreduction rate which was above 90%. The photoreduction rate of these chloroplasts remained to above 60% even after 30 day-storage at -20°C. In comparison with spinach, pandan leaves' chloroplasts possessed similar in vitro functional activity and storage stability at 4°C and -20°C. This warrants further investigation on chloroplasts of pandan leaves for higher-value applications.

  8. Phytoremediation: role of terrestrial plants and aquatic macrophytes in the remediation of radionuclides and heavy metal contaminated soil and water.

    Science.gov (United States)

    Sharma, Sunita; Singh, Bikram; Manchanda, V K

    2015-01-01

    Nuclear power reactors are operating in 31 countries around the world. Along with reactor operations, activities like mining, fuel fabrication, fuel reprocessing and military operations are the major contributors to the nuclear waste. The presence of a large number of fission products along with multiple oxidation state long-lived radionuclides such as neptunium ((237)Np), plutonium ((239)Pu), americium ((241/243)Am) and curium ((245)Cm) make the waste streams a potential radiological threat to the environment. Commonly high concentrations of cesium ((137)Cs) and strontium ((90)Sr) are found in a nuclear waste. These radionuclides are capable enough to produce potential health threat due to their long half-lives and effortless translocation into the human body. Besides the radionuclides, heavy metal contamination is also a serious issue. Heavy metals occur naturally in the earth crust and in low concentration, are also essential for the metabolism of living beings. Bioaccumulation of these heavy metals causes hazardous effects. These pollutants enter the human body directly via contaminated drinking water or through the food chain. This issue has drawn the attention of scientists throughout the world to device eco-friendly treatments to remediate the soil and water resources. Various physical and chemical treatments are being applied to clean the waste, but these techniques are quite expensive, complicated and comprise various side effects. One of the promising techniques, which has been pursued vigorously to overcome these demerits, is phytoremediation. The process is very effective, eco-friendly, easy and affordable. This technique utilizes the plants and its associated microbes to decontaminate the low and moderately contaminated sites efficiently. Many plant species are successfully used for remediation of contaminated soil and water systems. Remediation of these systems turns into a serious problem due to various anthropogenic activities that have

  9. Implementation and implications of macrophyte reconfiguration in hydraulic river modeling

    Science.gov (United States)

    Verschoren, Veerle; Schoelynck, Jonas; Buis, Kerst; Meire, Dieter; Bal, Kris; Meire, Patrick; Temmerman, Stijn

    2014-05-01

    In lowland rivers, abundant macrophyte growth can often be observed. The aquatic vegetation has an impact on the flow by creating friction which results in increased water levels and decreased flow velocities. At the same time submerged macrophytes are susceptible to hydrodynamic forces of the water. Their morphology is therefore often flexible and streamlined so that it enables reconfiguration (i.e. bending of macrophytes with water flow) and decreases potential damage at high flow velocities. Knowledge of these mutual interactions is crucial in order to model water flow in vegetated rivers. A correct estimation of flow velocity and water height is indispensable for the calculation of hydraulic, ecological and geomorphological parameters. The total resistance to water flow in a river can be described by a Manning coefficient. This value is influenced by river characteristics as well as by the presence of macrophytes. In this study a simple method is developed to quantify the resistance created by macrophytes after reconfiguration of their canopy. In order to achieve this we derive model formulations and plant parameters for three different macrophyte species and compare model simulation with measured flow velocity data for two case studies. Furthermore, the effect of macrophyte reconfiguration is investigated by modeling the same case studies with and without the implementation of macrophyte reconfiguration. It was found that the local resistance created by the vegetation was overestimated when reconfiguration was not considered. This resulted in an overestimation of stream velocity adjacent to the vegetation and an underestimation of the stream velocity within and behind the vegetation. Another effect was a higher water level gradient and consequently a higher Manning coefficient in the scenario without reconfiguration compared to the scenario with reconfiguration. Reconfiguration had also an influence on ecological and geomorphological parameters. It was found

  10. Microcosm wetlands for wastewater treatment with different hydraulic loading rates and macrophytes.

    Science.gov (United States)

    Jing, Shuh-Ren; Lin, Ying-Feng; Wang, Tze-Wen; Lee, Der-Yuan

    2002-01-01

    Constructed wetlands (CW) usually require large land areas for treating wastewater. This study evaluated the feasibility of applying CW with less land requirement by operating a group of microcosm wetlands at a hydraulic retention time (HRT) of less than 4 d in southern Taiwan. An artificial wastewater, simulating municipal wastewater containing 200 mg L(-1) of chemical oxygen demand (COD), 20 mg L(-1) of NH4+-N (AN), and 20 mg L(-1) of PO4(3-)-P (OP), was the inflow source. Three emergent plants [reed, Phragmites australis (Cav.) Trin. ex Steud.; water primrose, Ludwigia octovalvis (Jacq.) P.H. Raven; and dayflower, Commelina communis L.] and two floating plants [water spinach, Ipomoea aquatica Forssk.; and water lettuce, Pistia stratiotes L.] plants were tested. The planted systems showed more nutrient removal than unplanted systems; however, the type of macrophytes in CW did not make a major difference in treatment. At the HRTs of 2 to 4 d, the planted system maintained greater than 72,80, and 46% removal for COD, AN, and OP, respectively. For AN and OP removal, the highest efficiencies occurred at the HRT of 3 d, whereas maximum removal rates for AN and OP occurred at the HRT of 2 d. Both removal rates and efficiencies were reduced drastically at the HRT of 1 d. Removals of COD, OP, and AN followed first-order reactions within the HRTs of 1 to 4 d. The efficient removals of these constituents obtained with HRT between 2 and 4 d indicated the possibility of using a CW system for wastewater treatment with less land requirement.

  11. Ultrasound enhanced sanitizer efficacy in reduction of Escherichia coli O157:H7 population on spinach leaves

    Science.gov (United States)

    The use of ultrasound to enhance the efficacy of selected sanitizers in reduction of Escherichia coli O157:H7 populations on spinach was investigated. Spot-inoculated spinach samples were treated with water, chlorine, acidified sodium chlorite (ASC), peroxyacetic acid (POAA), and acidic electrolyzed...

  12. Macrophytes in estuarine gradients: flow through flexible vegetation

    NARCIS (Netherlands)

    Dijkstra, J.T.

    2012-01-01

    Aquatic plants –or macrophytes- are an important part of coastal, estuarine and freshwater ecosystems worldwide, both from an ecological and an engineering viewpoint. Their meadows provide a wide range of ecosystem services: forming a physical protection of the shoreline, enhancing water quality and

  13. Macrophyte presence and growth form influence macroinvertebrate community structure

    NARCIS (Netherlands)

    Walker, P.D.; Wijnhoven, S.; van der Velde, G.

    2013-01-01

    Multivariate analysis demonstrated that macroinvertebrate assemblages of macrophyte-dominated sub-habitats within a small eutrophic pond differed markedly from those of Bottom substrate and Open water habitats. Certain habitats (e.g. Nymphaea and Phragmites) appeared to be quite similar in their

  14. Long-term allelopathic control of phytoplankton by the submerged macrophyte Elodea nuttallii

    NARCIS (Netherlands)

    Vanderstukken, M.; Declerck, S.A.J.; Decaestecker, E.; Muylaert, K.

    2014-01-01

    Keywords: allelochemicals; chemical ecology; competition; nutrient limitation; shallow lakes Summary 1.It is well known that submerged macrophytes can suppress phytoplankton blooms in lakes and thus promote water quality and biodiversity. One of the possible mechanisms through which submerged macrop

  15. Long-term allelopathic control of phytoplankton by the submerged macrophyte Elodea nuttallii

    NARCIS (Netherlands)

    Vanderstukken, M.; Declerck, S.A.J.; Decaestecker, E.; Muylaert, K.

    2014-01-01

    Keywords: allelochemicals; chemical ecology; competition; nutrient limitation; shallow lakes Summary 1.It is well known that submerged macrophytes can suppress phytoplankton blooms in lakes and thus promote water quality and biodiversity. One of the possible mechanisms through which submerged

  16. Aquatic macrophytes as indicators of water quality in subtropical shallow lakes, Southern Brazil Macrófitas aquáticas como indicadores da qualidade da água em pequenos lagos rasos subtropicais, Sul do Brasil

    Directory of Open Access Journals (Sweden)

    Sabrina Amaral Pereira

    2012-03-01

    Full Text Available AIMS: We evaluated the potential of aquatic macrophyte communities as bioindicators in six small shallow lakes. METHODS: The sampling was conducted monthly for one year, during which all macrophytes were surveyed, and the water temperature, dissolved oxygen, pH, conductivity, total alkalinity, chlorophyll-a, suspended matter, total nitrogen (Nt and total phosphorus (Pt were measured. RESULTS: In total, forty-three species were recorded, and there were significant differences in the species richness and limnological conditions among the lakes studied. A canonical correspondence analysis showed that the concentration of nutrients (Nt and Pt, chlorophyll-a, suspended matter, dissolved oxygen and pH were the most important predictors of the distribution of macrophytes. Some emergents were related to the high concentration of nutrients, chlorophyll-a, and suspended matter. Moreover, the most submersed species were associated with environments with low nutrient concentrations and the lowest values chlorophyll-a and suspended matter. In addition, some species submerged and floating were related to low values pH, alkalinity and dissolved oxygen. Limnological differences between lakes may be cited as the main causes of the observed heterogeneous distribution of macrophytes. CONCLUSIONS: These results indicate the importance of limnological characteristics of the different environments in the macrophyte community composition and the potential role of this community as a bioindicator in shallow lakes in southern Brazil.OBJETIVO: Foi avaliado o potencial bioindicador da comunidade de macrófitas aquáticas em seis pequenos lagos rasos. MÉTODOS: O acompanhamento foi mensal durante um ano, sendo que em cada coleta, além do registro de todas as espécies de macrófitas foram determinadas a temperatura da água, oxigênio dissolvido, pH, condutividade elétrica, alcalinidade total, clorofila-a, material em suspensão, nitrogênio total (Nt e fósforo total

  17. The use of macrophytes for heavy metal pollution control in urban wetlands

    OpenAIRE

    Zhang, Tiantian

    1990-01-01

    A literature survey has been undertaken to identify the role and function of aquatic macrophytes in the removal of contaminants from polluted surface waters. The survey focussed primarily on heavy metals, however nitrate, phosphate and hydrocarbon removal are also discussed and the application of aquatic macrophytes in wastewater treatment systems is also described. The objectives of the research are: To determine heavy metal variations in water, sediment and plant tissues in both fiel...

  18. Monitoring the dynamics of an invasive emergent macrophyte community using operational remote sensing data

    Science.gov (United States)

    Albright, T.P.; Ode, D.J.

    2011-01-01

    Potamogeton crispus L. (curly pondweed) is a cosmopolitan aquatic macrophyte considered invasive in North America and elsewhere. Its range is expanding and, on individual water bodies, its coverage can be dynamic both within and among years. In this study, we evaluate the use of free and low-cost satellite remote sensing data to monitor a problematic emergent macrophyte community dominated by P. crispus. Between 2000 and 2006, we acquired eight satellite images of 24,000-ha Lake Sharpe, South Dakota (USA). During one of the dates for which satellite imagery was acquired, we sampled the lake for P. crispus and other emergent macrophytes using GPS and photography for documentation. We used cluster analysis to assist in classification of the satellite imagery and independently validated results using the field data. Resulting estimates of emergent macrophyte coverage ranged from less than 20 ha in 2002 to 245 ha in 2004. Accuracy assessment indicated 82% of image pixels were correctly classified, with errors being primarily due to failure to identify emergent macrophytes. These results emphasize the dynamic nature of P. crispus-dominated macrophyte communities and show how they can be effectively monitored over large areas using low-cost remote sensing imagery. While results may vary in other systems depending on water quality and local flora, such an approach could be applied elsewhere and for a variety of macrophyte communities. ?? Springer Science+Business Media B.V. 2010.

  19. Composition and abundance of fishes in the interface between open water and macrophyte banks, and the dynamics of this interface during morning and evening twilight, in lake Catalão, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Igor David da Costa

    2011-06-01

    Full Text Available This work studied the composition and abundance of the fishes that move between macrophyte banks and open water during the morning twilight (CM and afternoon twilight (CV. The collections were made using gillnets, along banks of Paspalum repens, at Catalão lake, in Amazonas, Brazil. A total of 222 individuals and 37 species were collected. Of these, 130 individuals were collected during the CM and 92 during the CV; 80 individuals were leaving during the CM and 40 individuals were leaving during the CV. Auchenipterus nuchalis, Pellona castelnaeana, Triportheus angulatus and T. albus were the most common and concentrated species collected in the CM and Pimelodus blochii was the most common species collected in the CV.

  20. Temporal and spatial distribution of macrophytes in the Gruža Reservoir (Serbia

    Directory of Open Access Journals (Sweden)

    Topuzović Marina

    2009-01-01

    Full Text Available A survey of aquatic macrophytes in the Gruža Reservoir (Serbia was conducted during the period 2007-2008. Detailed data on the actual condition of macrophytes were established and compared between four parts of the reservoir. The distributions of most aquatic macrophytes of the Gruža Reservoir are heterogeneous, with different values of the MMT and MMO indices and low values of the distribution ratio. Quantitative analysis indicates that the greatest diversity and abundance of species can be found in those parts of the reservoir with the shallowest water and greatest indentation of the shoreline.

  1. Isolation of chlorophyll a from spinach leaves

    Directory of Open Access Journals (Sweden)

    E.D. Dikio

    2008-08-01

    Full Text Available An efficient method for separating chlorophyll a from spinach leaves by column chromatography and solvent extraction techniques has been developed. The purity and identity of the chlorophyll a have been confirmed by UV-Vis, IR and mass spectrometry. Yields from 100 g of freeze-dried spinach were 23 – 24 mg of chlorophyll a.

  2. Biomass production by freshwater and marine macrophytes

    Energy Technology Data Exchange (ETDEWEB)

    North, W.J.; Gerard, V.A.; Kuwabara, J.S.

    1981-01-01

    Research on aquatic macrophytes as producers of biomass has been undertaken at Woods Hole Oceanographic Institution (WHOI) on the east coast and on the west coast by a group of collaborators in a joint effort known as the Marine Biomass Project. Studies at WHOI have focused on estuarine and coastal situations with some attention recently to freshwater plants. The Marine Farm Project has primarily been concerned with oceanic biomass production. A group at WHOI has undertaken a wide variety of studies concerning aquatic macrophytes including nutrient uptake, growth, yields, and environmental factors affecting yields. Aquatic biomass production systems have been surveyed on a worldwide basis and currently the role of carbon as a potential limiting nutrient in biomass culturing is being examined. The Marine Farm Project is presently attempting to grow giant kelp in offshore waters off southern California. Other work related to aquatic biomass production includes an investigation at the University of California, Berkeley, of microalgae in ponds. This paper will emphasize discussion of the kelp production phases of the Marine Farm Project. Activities by the WHOI are briefly summarized.

  3. 3种沉水植物对水体重金属镉去除效果的实验研究%Reaserch on phytoremediation of cadmium contaminated water by three submerged macrophytes

    Institute of Scientific and Technical Information of China (English)

    张饮江; 易冕; 王聪; 董悦; 刘晓培; 文晓峰; 李岩

    2012-01-01

    运用植物修复(phytoremediation)技术,选取金鱼藻(Ceratophyllum demersum L.)、伊乐藻(Elodea nuttallii)、苦草(Vallisnerianatans)3种沉水植物,比较研究不同pH与镉浓度对它们生长及去除镉效果的影响。结果表明,3种沉水植物均能耐受一定浓度范围的镉污染,并能不同程度地去除水体中的镉;在实验对照组中,金鱼藻与伊乐藻在pH为8.5、镉浓度为15μg/L的溶液中,去除镉的能力最强,苦草在pH为7.5、镉浓度为10μg/L的溶液中,去除镉的能力最强。在沉水植物活体中,伊乐藻对水体镉的抗性和去除率最大(达92%),金鱼藻次之(达90%),苦草最小(为66%);沉水植物粉对水体镉的去除效果较沉水植物活体显著,去除率表现为伊乐藻粉最大(达95%),金鱼藻粉次之(达93%),苦草最小(为88%),但苦草粉对镉的去除率随溶液镉浓度、pH等条件的变化波动较小,较稳定,为81%~88%。3种沉水植物适应范围广,高效无污染,可用于镉污染废水的植物修复。%The study is about the growth and cadmium′s restoration effect of three submerged macrophytes(Ceratophyllum demersum L.,Elodea nuttallii and Vallisneria natans)in different pH and cadmium.The result shows that,three submerged macrophytes can all tolerate the pollution of cadmium in a certain concentration range,and eliminate cadmium of the water to different degree.Ceratophyllum demersum L.and Elodea nuttallii have strongest elimination ability of cadmium under pH 8.5 or 15 μg/L cadmium.Vallisneria natans has strongest elimination ability of cadmium under pH 7.5 or 10 μg/L cadmium.Among these living submerged macrophytes,Elodea nuttallii has the strongest tolerate and elimination ability of cadmium(up to 92%),then Ceratophyllum demersum L.(up to 90%),and Vallisneria natans(up to 66%).The elimination ability of the 3 submerged macrophytes’ powder is more obvious than its living form,Elodea Canadensis

  4. Three-Gorge Dam influences wetland macrophytes in middle and lower reaches of Yangtze

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Dongting Lake, located in the middle and lower reaches of Yangtze, is regarded as a lake during the water swelling period and a continent in the ebbing period. The construction of the Three-Gorge Dam has influenced the water level of Dongting Lake and the growth of wetland macrophytes in the area. In this study, the growth of main species of wetland macrophytes, such as Carex and Phragmites at Dongting Lake, was observed, and the hydraulic gradient, one of the main factors dominating the abundance and distribution of wetland macrophytes, was also studied at six stations in Dongting Lake area and the effect of the Three-Gorge Dam construction on the growth of wetland macrophytes was observed.

  5. Regulation and role of epiphytic nitrification and denitrification in macrophyte-dominated systems

    Energy Technology Data Exchange (ETDEWEB)

    Eriksson, Peder G.

    2000-02-01

    This thesis examines mechanisms regulating bacterial nitrification and denitrification in attached microbial communities on surfaces of aquatic macrophytes. It also evaluates the role of epiphytic nitrification and denitrification for the nitrogen turnover in macrophyte-dominated nutrient-rich freshwater. Epiphytic nitrification is promoted in light and epiphytic denitrification occurs mainly in dark, because the metabolic activity of the aquatic macrophyte and its epiphytes induce in light high and in dark low oxygen concentrations in epiphytic communities. Epiphytic nitrification and denitrification are also affected by the physical and chemical characteristics of the aquatic macrophyte. The spatial distribution of nitrification in emergent macrophyte wetlands is related to the species composition of the emergent vegetation, possibly because of a macrophyte species-related release of organic nitrification inhibitors. Contrasting to nitrifying bacteria, which are lithotrophic, denitrifying bacteria use organic substances as an energy source and are therefore stimulated by the release of organic matter from aquatic macrophytes. Epiphytic communities support more denitrification in nutrient-rich than in nutrient-poor environments. In lakes and ponds, epiphytic denitrification is higher at sheltered locations than at locations exposed to wind-induced water movements or currents. In flowing water, epiphytic denitrification occurs mainly at low oxygen concentrations in the surrounding water. However, because aquatic macrophytes impede water flow and induce low oxygen concentrations in dark, epiphytic denitrification can be present within dense vegetation despite of high oxygen concentrations in the surrounding water. Epiphytic nitrification is almost unaffected by flow conditions, and can occur both in light and in dark. In shallow-water systems such as treatment wetlands, aquatic macrophytes often provide most of the accessible surface area for attached nitrifying and

  6. 土壤调理剂对红壤pH值及空心菜产量和品质的影响%Effects of soil conditioner on red soil pH and yield and quality of water spinach

    Institute of Scientific and Technical Information of China (English)

    李育鹏; 胡海燕; 李兆君; 孔庆波; 戴春山; 张永清

    2014-01-01

    Soil acidification is a key restricting factor to high and stable yields of crop. It also seriously affects the sustainable development of agriculture in China. In this paper, the effects of soil conditioner which mainly consisted of calcium carbonate on red soil pH, silica-alumina ratio and vegetable yield and quality were studied through field experiments. The results showed that soil conditioner could significantly increase soil pH and silica-alumina ratio, and could also improve water spinach yields and its quality. Compared with the control, soil pH was increased by 3. 60% ~21. 65%, water spinach yield was increased by 5. 81% ~44. 45%, vitamin C content was increased by 2. 48% ~23. 97%, and soluble sugar content was increased by 4. 18% ~26. 48% when the soil conditioner application concentration ranged from 600 to 1 800 kg/hm2 . Meanwhile, the ni-trate content of spinach had no significantly changes. It was found that the 1 500 kg/hm2 application amount was more reasona-ble. And, rotary tillage after surface fertilizer was a very reasonable and effective application method.%土壤酸化是制约土壤高产稳产的重要因素,严重影响了我国农业的可持续发展。通过大田试验研究了以碳酸钙为主要原料生产的土壤调理剂对酸性土壤pH值、硅铝率及空心菜产量和品质的影响。结果表明,土壤调理剂可以增加土壤pH值和硅铝率,提高空心菜的产量,改善空心菜的品质;与对照相比,土壤调理剂用量为600~1800 kg/hm2时,可使土壤pH值增加3.60%~21.65%,空心菜产量增加5.81%~44.45%,维生素C含量增加2.48%~23.97%,可溶性糖含量增加4.18%~26.48%;土壤调理剂对空心菜硝酸盐含量没有显著影响。研究发现,土壤调理剂用量为1500 kg/hm2时较为经济合理,表面撒施后旋耕是非常合理且有效的施用方法。

  7. Water quality effects of two aquatic macrophytes on eutrophic water from Lake Dianchi Caohai%两种水生植物对滇池草海富营养化水体水质的影响

    Institute of Scientific and Technical Information of China (English)

    王智; 张志勇; 张君倩; 闻学政; 王岩; 刘海琴; 严少华

    2013-01-01

    The water quality effects of two typical aquatic macrophytes, Eichhornia crassipes and Hydrilla verticillata, on the eutrophic water from Lake Dianchi Caohai were investigated by a series of microcosm experiments. The assimilation of nitrogen and phosphorus from microcosm by E. crassipes were 109% and 17% higher than that by H. verticillata, respectively. The leves of dissovle oxygen and pH in the E. crassipes treatments were significantly reduced, and electric conduction and redox potential were significantly increased comparing with the control. However, the results of H. verticillata treatments were opposive to E. crassipes treatments. During the experiment, the concentrations of total nitrogen (TN) and total phosphorus (TP) in treatments were significantly lower than that in control. With the same biomass of two aquatic plants used at the beginning of the experiment, the concentrations of TN, TP and chlorophyll-a (Chl-a) in H. verticillata treatments were significantly lower than that in E. crassipes treatments in the early stage of the experiment, but the results were reversed in the late stage of the experiment. After aquatic macrophytes harvest, concentrations of TN and TP could maintain the levels of which before harvest; Chl-a concentrations in E. crassipes treatments could also maintain the level of which before harvest, but in H. verticillata treatments, Chl-a concentrations showed a significant increase.%通过模拟实验,比较了2种典型水生植物水葫芦和轮叶黑藻对滇池草海富营养化水体水质的影响.结果表明,在实验过程中,水葫芦同化吸收的氮、磷量分别比轮叶黑藻所同化吸收的量高109%和17%.在水生植物采收前,水葫芦处理组水体DO和pH值显著性低于对照组,电导率(EC)和氧化还原电位(Eh)显著性高于对照组,与轮叶黑藻处理组结果相反.水葫芦和轮叶黑藻处理组水体TN和TP浓度均显著低于对照组;相同初始种养量的情况下,试验初

  8. Responses of aquatic macrophyte cover and productivity to flooding variability on the Amazon floodplain.

    Science.gov (United States)

    Silva, Thiago S F; Melack, John M; Novo, Evlyn M L M

    2013-11-01

    Macrophyte net primary productivity (NPP) is a significant but understudied component of the carbon budget in large Amazonian floodplains. Annual NPP is determined by the interaction between stem elongation (vertical growth) and plant cover changes (horizontal expansion), each affected differently by flood duration and amplitude. Therefore, hydrological changes as predicted for the Amazon basin could result in significant changes in annual macrophyte NPP. This study investigates the responses of macrophyte horizontal expansion and vertical growth to flooding variability, and its possible effects on the contribution of macrophytes to the carbon budget of Amazonian floodplains. Monthly macrophyte cover was estimated using satellite imagery for the 2003-2004 and 2004-2005 hydrological years, and biomass was measured in situ between 2003 and 2004. Regression models between macrophyte variables and river-stage data were used to build a semiempirical model of macrophyte NPP as a function of water level. Historical river-stage records (1970-2011) were used to simulate variations in NPP, as a function of annual flooding. Vertical growth varied by a factor of ca. 2 over the simulated years, whereas minimum and maximum annual cover varied by ca. 3.5 and 1.5, respectively. Results suggest that these processes act in opposite directions to determine macrophyte NPP, with larger sensitivity to changes in vertical growth, and thus maximum flooding levels. Years with uncommonly large flooding amplitude resulted in the highest NPP values, as both horizontal expansion and vertical growth were enhanced under these conditions. Over the simulated period, annual NPP varied by ca. 1.5 (1.06-1.63 TgC yr(-1) ). A small increasing trend in flooding amplitude, and by extension NPP, was observed for the studied period. Variability in growth rates caused by local biotic and abiotic factors, and the lack of knowledge on macrophyte physiological responses to extreme hydrological conditions

  9. Residue Determination of Dimethoate in Leafy Vegetables (Spinach using RPHPLC

    Directory of Open Access Journals (Sweden)

    J. BAGYALAKSHMI,

    2011-02-01

    Full Text Available Residues of Dimethoate present in locally available Spinach varities - Palak : Beta maritima, Vendhai : Trigonella foenumgraecum, Thandan : Amarnathus mangostanus - green and red were determined. The quantification was carried out using RP-HPLC for unprocessed (fresh and processed (washed, washed and cooked Spinach samples. The extraction was carried out with benzene. It was found that unprocessedDimethoate Spinach contains dimethoate residue which is slightly more than the tolerance limit, while the processed sample contains imethoate residue within the tolerance limit. The washed samples (3 times under ordinary tap water showed 28-50 % reduction while the cooked samples (boiling for 15 min shows 58-71 % reduction compared to the unprocessed samples. Hence it can be concluded that in case of greens, it should be washed three times with ordinary water and then cooked (cooked for fifteen minutes so that the pesticide residue is very much reduced and it will be well within the tolerance limit as specified by EPA (Environmental Protection Agency.

  10. Non-effect of water hardness on the accumulation and toxicity of copper in a freshwater macrophyte (Ceratophyllum demersum): how useful are hardness-modified copper guidelines for protecting freshwater biota?

    Science.gov (United States)

    Markich, Scott J; King, Angus R; Wilson, Scott P

    2006-12-01

    Several nations have adopted hardness-modified copper (Cu) guidelines for protecting freshwater biota. However, there is a lack of good quality data and mechanistic understanding on the effects of true water hardness (calcium (Ca) and magnesium (Mg)) on the bioavailability and toxicity of Cu to freshwater biota, particularly macrophytes. This study determined the effect of true water hardness (35, 90 and 335 mg CaCO(3)/l, added as Ca and Mg chloride in a 1:1 mole ratio) on the cell surface binding affinity (log K), accumulation and toxicity (96 h growth (biomass and stem length) and photosynthetic pigment inhibition) of Cu in the free-floating submerged macrophyte, Ceratophyllum demersum, in a synthetic freshwater with constant alkalinity (16 mg CaCO(3)/l) and pH (7.0). There were no significant (P>0.05) differences in the cell surface binding affinity, accumulation or toxicity of Cu in C. demersum with a 10-fold increase in water hardness from 35 to 335 mg CaCO(3)/l. The mean 96 h EC(50) values (and 95% confidence intervals) for biomass, the most sensitive endpoint, were 8.4 (7.6-9.2), 8.9 (8.0-9.8) and 9.9 (9.1-10.7) microg/l Cu for 35, 90 and 335 mg CaCO(3)/l, respectively. Speciation calculations indicated only very small (1-6%) differences in the percentage distribution (i.e. bioavailability) of Cu over the hardness range. These collective results indicate no apparent competition between Cu and Ca/Mg for binding sites on the cell surface. Given that the mechanism of Cu uptake (via Cu-specific and Na-linked transporters) is fundamentally different to that of Cd, Ni, Pb and Zn (via Ca transporters), for which other hardness-dependent algorithms have been developed, it is doubtful whether a hardness-modified Cu guideline value will be sufficiently protective of sensitive freshwater biota, such as C. demersum, particularly in medium-hard fresh surface waters with low levels of dissolved organic carbon. The biotic ligand model offers a more flexible and mechanistic

  11. Inhibition of spinach bolting by growth regulators

    Directory of Open Access Journals (Sweden)

    Jan Borkowski

    2015-06-01

    Full Text Available Spinach (Spinacia oleracea L. plants must be harvested during a short period of time because they bolt just after producing some edible leaves. Maleic hydrazide (MH and its commercial preparation "Antyrost" were found to inhibit bolting very strongly. The preparation Off-shoot-O showed very weak activity in suppressing bolting but diminished markedly the resistance of spinach plants to fungus diseases. Triiodobenzoic acid stimulated bolting, and the retardant succinic acid-2-2-dimethylhydrazide (SADH did not affect bolting. Application of MH to inhibit spinach bolting cannot be recommended in practice before investigating the residues of this compound in leaves.

  12. PIXE analysis of chromium phytoaccumulation by the aquatic macrophytes Eicchornia crassipes

    Science.gov (United States)

    Espinoza-Quiñones, F. R.; Rizzutto, M. A.; Added, N.; Tabacniks, M. H.; Módenes, A. N.; Palácio, S. M.; Silva, E. A.; Rossi, F. L.; Martin, N.; Szymanski, N.

    2009-04-01

    The uptake of hexavalent chromium in free living floating aquatic macrophytes Eicchornia crassipes cultivated in non-toxic chromium-doped hydroponic solutions is presented. A Cr-uptake bioaccumulation experiment was carried out using healthy macrophytes grown in a temperature controlled greenhouse. Six samples of nutrient media and plants were collected during the 23 day experiment. Roots and leaves were acid digested with the addition of an internal Gallium standard, for thin film sample preparation and quantitative Cr analysis by PIXE method. The Cr 6+ mass uptake by the macrophytes reached up to 70% of the initial concentration, comparable to former results and literature data. The Cr-uptake data were described using a non-structural first order kinetic model. Due to low cost and high removal efficiency, living aquatic macrophytes E. crassipes are a viable biosorbent in an artificial wetland of a water effluent treatment plant.

  13. PIXE analysis of chromium phytoaccumulation by the aquatic macrophytes Eicchornia crassipes

    Energy Technology Data Exchange (ETDEWEB)

    Espinoza-Quinones, F.R. [Department of Chemical Engineering - Postgraduate Program - NBQ, West Parana State University, Rua da Faculdade, 645, Jardim Santa Maria, 85903-000 Toledo, Parana (Brazil)], E-mail: f.espinoza@terra.com.br; Rizzutto, M.A.; Added, N.; Tabacniks, M.H. [Physics Institute, University of Sao Paulo, Rua do Matao s/n, Travessa R 187, 05508-900 Sao Paulo, SP (Brazil); Modenes, A.N.; Palacio, S.M.; Silva, E.A.; Rossi, F.L.; Martin, N.; Szymanski, N. [Department of Chemical Engineering - Postgraduate Program - NBQ, West Parana State University, Rua da Faculdade, 645, Jardim Santa Maria, 85903-000 Toledo, Parana (Brazil)

    2009-04-15

    The uptake of hexavalent chromium in free living floating aquatic macrophytes Eicchornia crassipes cultivated in non-toxic chromium-doped hydroponic solutions is presented. A Cr-uptake bioaccumulation experiment was carried out using healthy macrophytes grown in a temperature controlled greenhouse. Six samples of nutrient media and plants were collected during the 23 day experiment. Roots and leaves were acid digested with the addition of an internal Gallium standard, for thin film sample preparation and quantitative Cr analysis by PIXE method. The Cr{sup 6+} mass uptake by the macrophytes reached up to 70% of the initial concentration, comparable to former results and literature data. The Cr-uptake data were described using a non-structural first order kinetic model. Due to low cost and high removal efficiency, living aquatic macrophytes E. crassipes are a viable biosorbent in an artificial wetland of a water effluent treatment plant.

  14. Nitrogen or phosphorus limitation in lakes and its impact on phytoplankton biomass and submerged macrophyte cover

    DEFF Research Database (Denmark)

    Søndergaard, Martin; Lauridsen, Torben Linding; Johansson, Liselotte Sander

    2017-01-01

    macrophyte cover. We conclude that P is of key importance for the ecological quality of Danish lakes but that increased N concentrations, particularly in shallow lakes with moderate to high TP, may have significantly adverse effects on lake water quality and ecological status in summer.......We used data on nutrients, chlorophyll a (Chla) and submerged macrophyte cover from up to 817 Danish lakes to elucidate seasonal variations in nitrogen (N) and phosphorus (P) concentrations and to study the impact of N or its role in combination with P. In both deep and shallow lakes, we found...... related more strongly to TP than to TN, but at high TP concentrations TN explained more of the variability in Chla than TP. Macrophyte cover tended to decrease at increasing TN when TP was between 0.1 and 0.4 mg/l. At macrophyte cover above 20%, Chla was considerably lower compared with lakes with low...

  15. Applied study of biofilm carrier-macrophyte assemblage in strengthening river water treatment%生物填料-沉水植物联用在河道水强化处理中的应用研究

    Institute of Scientific and Technical Information of China (English)

    潘静赟; 李正魁

    2012-01-01

    采用新型亲水性和疏水性材料作为生物填料,研究了生物填料-沉水植物联用技术在静态和原位水体中对武进港红旗河的水强化处理性能。静态实验选用2种生物填料考察其水处理特性,筛选出性能较好的填料进行了原位实验,沉水植物选取原位常见的狐尾藻、金鱼藻和伊乐藻。通过原位实验进一步考察了所选生物填料性能及生物填料-沉水植物联用对河道水的处理效果。结果表明,原位实验处理效果优于静态实验,原位实验中各测定指标的总平均去除率分别为:氨氮69.07%,亚硝氮70.28%,硝态氮47.58%,总氮53.28%,正磷酸盐84.88%,总磷83.50%。填料平均挂膜速率为453.01 nmol/(g.d)。%New type of hydrophilic and hydrophobic materials were selected as biofilm carriers,and the water treatment properties of biofim carrier-macrophyte assemblage technology was studied under static flow pattern and in situ water column of Hongqi River.The aim of static experiment was to select biofilm carrier with better performance of water purification for the next in situ river experiment.In the study,local popular macrophytes of Myriophyllum spicatum,Ceratophyllum demersum and Elodea nuttallii were chosen for the experiment.The in situ experiment was conducted to further investigate the properties of selected biofilm carrier and the rejoined purification effect of biofim carrier-macrophyte assemblage in Hongqi River.The results showed that it had better water treatment effect during in situ experiment,and the average removal percentages of NH+4-N,NO-2-N,NO-3-N,total N(TN),PO3-4and total P(TP) were 69.07%,70.28%,47.58%,53.28%,84.88% and 83.50%,respectively.The average rate of biofilm culturing was 453.01 nmol/(g·d).

  16. Effects of Simulated Acid Rain on the Migration and Transformation of Hexavalent Chromium in a Water Spinach-soil System%模拟酸雨对空心菜-土壤系统中六价铬迁移转化的影响

    Institute of Scientific and Technical Information of China (English)

    陈桂葵; 李志鹏; 陈恋; 刘明中; 黎华寿

    2013-01-01

    A pot experiment was conducted to study the effects of acid rain on the distribution, migration and transformation of hexavalent chromium in a water spinach(Ipomoea aquatica) soil system. Results showed that the amount of total chromium in water spinach treated with simulated acid rain was significantly higher than those without acid rain. The content of chromium in the underground part of water spinach was significantly higher than it in the aboveground part in treatments with simulated acid rain. While water spinach could survive and grow normally in soil with 100 mg·kg-1 chromium, they couldn't survive in soil with chromium up to 200 mg·kg-1. Valences of more than 97.0% added hexavalent chromium was changed. The majority portion of the chromium was remained in soil. Only very small portion was absorbed by water spinach. The simulated acid rain could significantly enhance the absorption rate of chromium by water spinach. The amount of absorbed chromium in treatments with acid rain was 2.6 to 5 times as those in the treatments without acid rain. The chromium enrichment coefficients were 2.2 and 4.6 for the aboveground part and the underground part of water spinach respectively by comparing the treatments with and without acid rain. The research result shows that acid rain increases the risk of crop grown in soil contaminated by chromium especially through the absorption of chromium by root system. Hence, agricultural food safety problem in chromium polluted area will be enhanced by acid rain.%通过盆栽试验,研究了模拟酸雨条件下六价铬在土壤和空心菜之间的分布、迁移和转化规律.结果发现,酸雨处理显著提高了空心菜体内铬的含量,其中空心菜地下部的铬含量显著高于其地上部;空心菜在100 mg·kg-1的铬处理下仍然可以正常生长,但当土壤中的铬达到200 mg· kg-1时,空心菜生长数天后即全部死亡;添加到土壤中的六价铬97.0%以上已经转化成其他形态而留

  17. Reconstruction and water purification of submerged macrophytes community in eutrophicated Gehu Lake during summer%夏季富营养化滆湖中沉水植物群落重建及水质净化效果

    Institute of Scientific and Technical Information of China (English)

    黄峰; 李勇; 潘继征; 陶花; 赵海光

    2011-01-01

    通过滆湖原位围隔试验分析了不同单种及混种沉水植物群落随环境变化的生长状况及对水质的净化效果.结果表明:(1)夏季,沉水植物生物量的平均值与透明度/水位存在显著线性相关性,进一步表明沉水植物的生长在很大程度上依赖于光强和水深.(2)各植物区(除马来眼子菜区外)的高锰酸盐指数、TN高于空白对照区.(3)对比空白对照区,苦草区、金鱼藻区、金鱼藻—伊乐藻区对NH3-N、TP、叶绿素a的去除率都在25%以上.对比敞水区,金鱼藻—伊乐藻区、苦草—马来眼子菜区、苦草—伊乐藻区对TP的去除率分别为33.5%、20.8%、9.9%;金鱼藻—伊乐藻区对叶绿素a的去除率为41.4%.(4)TP和叶绿素a存在显著线性关系,反映了沉水植物可通过对TP的去除有效控制藻类的生长.(5)苦草、金鱼藻—伊乐藻群落、苦草—马来眼子菜群落对水体中NH3-N、TP、叶绿素a的平均去除速率(V)都符合V-时间(t)回归方程.(6)在沉水植物群落恢复中可优先考虑金鱼藻—伊乐藻、苦草—马来眼子菜群落等有稳定及较高平均去除速率的沉水植物群落重建形式.%In-situ enclosure experiment was conducted in Genu Lake to investigate the effect of environmental change on growth and purification performance of different single and mixture submerged macrophytes community. Results showed that the average biomass of submerged macrophytes was significant linearly correlated with transparency and water level, which indicated that the growth of submerged macrophytes was highly dependent on the light intensity and water depth. The permanganate index and TN in test area were higher than that in control area except for the test area planting Potamogeton malaianus. Compared with control area, planting Vallisneria spiralis, Cera-tophyllum demersum and Ceratophyllum demersum-Elodea nuttallii could removal more than 25% of NH3-N, TP and chlorophyll-a; Compared

  18. Effects of hybrid planting of emergent and submerged macrophytes on water quality of Beidagang Reservoir%挺水植物与沉水植物组配对北大港水库水质的影响

    Institute of Scientific and Technical Information of China (English)

    常素云; 赵静静; 刘小川; 罗莎; 刘波

    2014-01-01

    As local dominant species in the Beidagang Reservoir , the emergent macrophytes , Typha, and the euryhaline submerged macrophytes , including Labyrinth potamogetonaceae, Ceratophyllum, and Myriophyllum were selected to study the effects of hybrid planting of Typha and various submerged plants on water purification .The results show that the best removal effect on NH 3-N and TP can be achieved by hybrid planting of Typha and Labyrinth potamogetonaceae, with the removal rates being 62.1% and 83.9%, respectively .The best removal effect on TN and COD can be achieved by hybrid planting of Typha and Ceratophyllum, with the removal rates being 93.3%and 30.4%, respectively .Matlab software was used to analyze the water quality after experiments . The results show that hybrid planting of Typha and Ceratophyllum can improve the water purification capability to the greatest extent .%选取北大港水库当地优势种挺水植物香蒲和广盐性沉水植物篦齿眼子菜、金鱼藻、狐尾藻为研究对象,研究香蒲与不同沉水植物的组配试验对水体净化效果的影响。结果表明:香蒲与篦齿眼子菜组配对水体NH3-N、TP的去除效果最好,去除率分别为62.1%、83.9%;香蒲与金鱼藻组配对TN、COD的去除效果最佳,去除率分别为93.3%、30.4%。利用Matlab软件平台对试验结束时的水质进行分析,发现香蒲与金鱼藻组配方式对水体的净化能力最强。

  19. Emergent Macrophytes Support Zooplankton in a Shallow Tropical Lake: A Basis for Wetland Conservation.

    Science.gov (United States)

    Gebrehiwot, Mesfin; Kifle, Demeke; Triest, Ludwig

    2017-09-08

    Understanding the biodiversity value of littoral zones of lakes is a priority for aquatic biodiversity conservation. However, less emphasis has been given to the littoral part of tropical African lakes, with many of the previous researches focusing only on the open water side. The aim of the present study was, therefore, to investigate the impact of the littoral zone of a shallow freshwater tropical lake (Ziway, Ethiopia), dominated by two emergent macrophytes, on zooplankton community structure. We hypothesized that the wetland vegetation serves as a preferred microhabitat for zooplankton communities. A lake with substantial coverage of emergent macrophytes was monitored monthly from January to August, 2016. The monitoring included the measurements of physical, chemical, and biological parameters. Sampling sites were selected to represent areas of the macrophyte vegetation (Typha latifolia and Phragmites australis) and the open water part of the lake. Sites with macrophyte vegetation were found to be the home of more dense and diverse zooplankton community. However, during the period of high vegetation loss, the density of crustacean zooplankton showed significant reduction within the patches of macrophytes. From biodiversity conservation perspective, it was concluded that the preservation of such small areas of macrophytes covering the littoral zone of lakes could be as important as protecting the whole lake. However, the rapid degradation of wetland vegetation by human activities is a real threat to the lake ecosystem. In the not-too-far future, it could displace and evict riparian vegetation and the biota it supports.

  20. Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

    Science.gov (United States)

    Zhang, Songhe; Pang, Si; Wang, Peifang; Wang, Chao; Guo, Chuan; Addo, Felix Gyawu; Li, Yi

    2016-10-01

    Submerged macrophytes play important roles in constructed wetlands and natural water bodies, as these organisms remove nutrients and provide large surfaces for biofilms, which are beneficial for nitrogen removal, particularly from submerged macrophyte-dominated water columns. However, information on the responses of biofilms to submerged macrophytes and nitrogen molecules is limited. In the present study, bacterial community structure and denitrifiers were investigated in biofilms on the leaves of four submerged macrophytes and artificial plants exposed to two nitrate concentrations. The biofilm cells were evenly distributed on artificial plants but appeared in microcolonies on the surfaces of submerged macrophytes. Proteobacteria was the most abundant phylum in all samples, accounting for 27.3-64.8% of the high-quality bacterial reads, followed by Chloroflexi (3.7-25.4%), Firmicutes (3.0-20.1%), Acidobacteria (2.7-15.7%), Actinobacteria (2.2-8.7%), Bacteroidetes (0.5-9.7%), and Verrucomicrobia (2.4-5.2%). Cluster analysis showed that bacterial community structure can be significantly different on macrophytes versus from those on artificial plants. Redundancy analysis showed that electrical conductivity and nitrate concentration were positively correlated with Shannon index and operational taxonomic unit (OTU) richness (log10 transformed) but somewhat negatively correlated with microbial density. The relative abundances of five denitrifying genes were positively correlated with nitrate concentration and electrical conductivity but negatively correlated with dissolved oxygen.

  1. Macrophytes: Freshwater Forests of Lakes and Rivers.

    Science.gov (United States)

    McDermid, Karla J.; Naiman, Robert J.

    1983-01-01

    Physical, chemical, and biological effects on macrophytes (aquatic plants) on the freshwater ecosystem are discussed. Research questions and issues related to these organisms are also discussed, including adaptations for survival in a wet environment, ecological consequences of large-scale macrophyte eradication, seasonal changes in plant…

  2. Efficacy of chlorine and acidified sodium chlorite on microbial population and quality changes of spinach leaves.

    Science.gov (United States)

    Nei, Daisuke; Choi, Ji-Weon; Bari, Md Latiful; Kawasaki, Susumu; Kawamoto, Shinichi; Inatsu, Yasuhiro

    2009-06-01

    Efficacy of washing with distilled water, chlorine solution, and acidified sodium chlorite (ASC) solution on populations of microorganisms on spinach leaves was evaluated. Washing with chlorine (100 mg/L) and ASC (sodium chlorite, 15 mg/L; citric acid, 200 mg/L) resulted in significant population reduction (1.1-1.9 log CFU/g) of aerobic microflora, coliform, and Escherichia coli O157:H7 (p sodium chlorite chemicals have been commercially available, and no difference in decontamination efficacy among the chemicals was observed when same concentration of sodium chlorite and citric acid were used. In addition, the reduction of E. coli O157:H7 population was influenced depending on the inoculation method and type of washing. It has been seen that dip-inoculated spinach leaves showed lower reduction than that of spot-inoculated spinach. After washing, populations of aerobic microflora, coliform, and E. coli O157:H7 were increased during storage at 10 degrees C, and washing condition before storage did not affect the subsequent increases in microbial population. Color of spinach leaves washed with ASC solution was not different from the color of those washed with water or chlorine solution, and washing with ASC solution was concluded to has no effect on appearance of spinach leaves.

  3. Physical and chemical properties of substrates produced using macrophytes aquatics

    Directory of Open Access Journals (Sweden)

    Walda Monteiro Farias

    2013-12-01

    Full Text Available Aquatic macrophytes are widely used as bioindicators of water quality because their proliferation usually occurs in eutrophic water sources and has hit several parts of Brazil and the world, restricted the multiple uses of aquatic ecosystems. However, this group of plants is able to retain considerable amounts of nutrients, presenting high productivity and high growth rate, thus, a good source of biomass for use in the production of substrates. In order to evaluate the potential of aquatic macrophytes water hyacinth (Eichhornia crassipes Solms., water lettuce (Pistia stratiotes L. and cattail (Typha domingensis Pers. in the production of substrates was performed in this work, the physical and chemical characterization and evaluation of the degree of humification. The treatments were arranged in a 3 × 4 factorial, completely randomized design with three replications. All substrates produced with 100% macrophyte density present within the limits of 400 kg m-3, considered ideal. The composite substrates with water hyacinth and water lettuce are with the electrical conductivity of 0,79 a 2,49 dS m-1 within recommended. organic compounds produced are considered mature and have high levels of nitrogen phosphorus and potassium; The substrate produced with 70% water lettuce +30 % dung and 70% composed of cattail manure +20% +10% topsoil and 70 +30% cattail manure have C/N ratio within the considered ideal; the humification ratio and humification index, except for the four treatments (70 % water lettuce manure +30%, 5 (100% water hyacinth and 8 (70% water hyacinth manure +30% are within the considered ideal, the percentage of humic acids and polymerization rate, except for treatments 1 (100% water lettuce and 12 (100% cattail, are shown below the ideal.

  4. A preliminary evaluation of lake morphometric traits influence on the maximum growing depth of macrophytes

    Directory of Open Access Journals (Sweden)

    Mattia M. Azzella

    2014-03-01

    Full Text Available Underwater light regime is widely considered the principal determinant of aquatic plant depth distribution. The majority of previous studies dealing with macrophytes in lakes have singled out Secchi disk transparency (SD values as the key empirical proxy to explain the maximum depth of macrophyte colonization (Zc. Few studies have investigated the role played by lake morphometry in structuring macrophyte beds. Using a balanced dataset including 20 Italian lakes (10 shallow and 10 deep lakes, we analysed transparency and lake morphometric traits to investigate their possible effects on Zc. Our results demonstrate that lake area plays a significant role, and confirm a direct influence of SD values on Zc. Considering lakes with an equal degree of transparency, smaller lakes may yield a lower Zc than larger ones. Morphology has a great influence on lake ecological characteristics especially on water thermal conditions and mixing depth. Based on our data, we argue that the thermal stratification plays a non negligible role in explaining macrophytes zonation, due to its influence on macrophytes life cycles and phytoplankton vertical distribution. Therefore, the present data suggest the need to enhance and refine our knowledge about the relationship between aquatic plants distribution and lake thermal conditions to better model the response of macrophytes to climate change and eutrophication.

  5. Macrophyte succession in floodplain lakes : spatio-temporal patterns in relation to river hydrology, lake morphology and management

    NARCIS (Netherlands)

    Geest, van G.J.

    2005-01-01

    Two questions are central to the work in this thesis: 1. What are the factors determining macrophyte composition in floodplain lakes along the Lower Rhine? 2. What advice can be given for design of future water bodies?

  6. Spatial and temporal dynamics of macrophyte cover in a large regulated river.

    Science.gov (United States)

    Tena, A; Vericat, D; Gonzalo, L E; Batalla, R J

    2016-11-24

    The River Ebro basin is extensively dammed. Dams alter the geomorphological functioning of the river by altering its flow regime (e.g. reducing mean and maximum discharges), increasing bed stability (armouring) and decreasing turbidity (water clarity). These effects, together with an increase in nutrient concentrations and water temperature, have generated optimal conditions for the proliferation of aquatic macrophytes. In this paper, we analyse the temporal and spatial changes of macrophyte cover in the lowermost Ebro through a series of field campaigns carried out between 2009 and 2010. Special attention was paid to the spatial distribution of macrophytes in relation to flow hydraulics, channel geometry and bed sedimentology. Temporal changes in macrophyte cover were analysed in relation to the frequency and magnitude of both natural floods and flushing flows (artificial flow releases from dams with generally a magnitude that equates around a2-year flood in the river). Spatially, the proportion of macrophytes along the reaches showed a variable pattern, with a succession of areas with both high and low plant density, coinciding with the alternation of riffles and pools in the channel. The highest values of plant cover (>65%) occurred in riffles and in transition to riffle areas, while the lowest densities (1% or almost negligible) were observed in pools and transition to pool areas. Water depth and the grain-size distribution of the riverbed materials (i.e. D84), are found to be the main factors controlling the degree of plant cover in the lower Ebro. Temporally, the macrophyte proportion varied during the hydrological year, with a clear increment from late spring to early autumn (i.e. vegetation cover reached 40%, on average, of the channel surface). Macrophyte coverage decreases immediately following a flushing flow but in the long term, vegetation re-occupied the area again, even slightly increasing in some sections; overall, the mean percentage of macrophyte

  7. Temporal trends and effects of diversity on occurrence of exotic macrophytes in a large reservoir

    Science.gov (United States)

    Thomaz, Sidinei Magela; Carvalho, Priscilla; Mormul, Roger Paulo; Ferreira, Fernando Alves; Silveira, Márcio José; Michelan, Thaísa Sala

    2009-09-01

    Two exotic invasive macrophyte species (the emergent Urochloa subquadripara - tenner-grass - and the submersed Hydrilla verticillata - hydrilla) were investigated in a large sub-tropical reservoir. We analyzed their occurrences over an extended period and tested the hypothesis that macrophyte richness decreases their invasibility. The alternative hypothesis that the occurrence of these exotics is affected by fetch and underwater radiation (important determinants of macrophyte assemblage composition in this reservoir) was also tested. Incidence data (presence/absence) was obtained over 9.5 years at 235 stations. Logistic regression was applied to test whether the likelihood of occurrence of these two species was affected by macrophyte richness, fetch or underwater radiation. Tenner-grass was recorded at a high frequency and quickly recovered from disturbances caused by water drawdown. In contrast, H. verticillata was first recorded in 3 sites in January 2007, but it spread quickly, reaching 30.5% of the sites 19 months later. The main channel of the Paraná River was the main source of propagules for this species. The likelihood of occurrence of tenner-grass was positively affected by macrophyte richness but negatively affected by fetch. Thus, wave disturbance is probably more important than diversity in preventing invasion by this species. Hydrilla, by contrast, was negatively affected by macrophyte richness and positively affected by fetch and underwater radiation. Although this result might indicate that macrophyte diversity prevents hydrilla invasion, this is probably not true because hydrilla colonized deeper sites where few species of plant exist. Resistance to disturbances caused by water drawdown (tenner-grass) and waves (hydrilla) as well as persistency of tenner-grass and fast spread of hydrilla make these exotic species a cause for concern because of their potential impacts on water uses and maintenance of diversity.

  8. Abscisic acid accumulation in spinach leaf slices in the presence of penetrating and nonpenetrating solutes

    Energy Technology Data Exchange (ETDEWEB)

    Creelman, R.A.; Zeevaart, J.A.D.

    1985-01-01

    Abscisic acid (ABA) accumulated in detached, wilted leaves of spinach (Spinacia oleracea L. cv Savoy Hybrid 612) and reached a maximum level within 3 to 4 hours. The increase in ABA over that found in detached turgid leaves was approximately 10-fold. The effects of water stress could be mimicked by the use of thin slices of spinach leaves incubated in the presence of 0.6 molar mannitol, a compound which causes plasmolysis (loss of turgor). When spinach leaf slices were incubated with ethylene glycol, a compound which rapidly penetrates the cell membrane causing a decrease in the osmotic potential of the tissue and only transient loss of turgor, no ABA accumulated. Spinach leaf slices incubated in both ethylene glycol and mannitol had ABA levels similar to those found when slices were incubated with mannitol alone. Increases similar to those found with mannitol also occurred when Aquacide III, a highly purified form of polyethylene glycol, was used. When spinach leaf slices were incubated with solutes which are supposed to disturb membrane integrity no increase in ABA was observed. These data indicate that, with respect to the accumulation of ABA, mannitol caused a physical stress rather than a chemical stress.

  9. Vulnerabilities of macrophytes distribution due to climate change

    Science.gov (United States)

    Hossain, Kaizar; Yadav, Sarita; Quaik, Shlrene; Pant, Gaurav; Maruthi, A. Y.; Ismail, Norli

    2017-08-01

    The rise in the earth's surface and water temperature is part of the effect of climatic change that has been observed for the last decade. The rates of climate change are unprecedented, and biological responses to these changes have also been prominent in all levels of species, communities and ecosystems. Aquatic-terrestrial ecotones are vulnerable to climate change, and degradation of the emergent aquatic macrophyte zone would have contributed severe ecological consequences for freshwater, wetland and terrestrial ecosystems. Most researches on climate change effects on biodiversity are contemplating on the terrestrial realm, and considerable changes in terrestrial biodiversity and species' distributions have been detected in response to climate change. This is unfortunate, given the importance of aquatic systems for providing ecosystem goods and services. Thus, if researchers were able to identify early-warning indicators of anthropogenic environmental changes on aquatic species, communities and ecosystems, it would certainly help to manage and conserve these systems in a sustainable way. One of such early-warning indicators concerns the expansion of emergent macrophytes in aquatic-terrestrial ecotones. Hence, this review highlights the impact of climatic changes towards aquatic macrophytes and their possible environmental implications.

  10. Vulnerabilities of macrophytes distribution due to climate change

    Science.gov (United States)

    Hossain, Kaizar; Yadav, Sarita; Quaik, Shlrene; Pant, Gaurav; Maruthi, A. Y.; Ismail, Norli

    2016-06-01

    The rise in the earth's surface and water temperature is part of the effect of climatic change that has been observed for the last decade. The rates of climate change are unprecedented, and biological responses to these changes have also been prominent in all levels of species, communities and ecosystems. Aquatic-terrestrial ecotones are vulnerable to climate change, and degradation of the emergent aquatic macrophyte zone would have contributed severe ecological consequences for freshwater, wetland and terrestrial ecosystems. Most researches on climate change effects on biodiversity are contemplating on the terrestrial realm, and considerable changes in terrestrial biodiversity and species' distributions have been detected in response to climate change. This is unfortunate, given the importance of aquatic systems for providing ecosystem goods and services. Thus, if researchers were able to identify early-warning indicators of anthropogenic environmental changes on aquatic species, communities and ecosystems, it would certainly help to manage and conserve these systems in a sustainable way. One of such early-warning indicators concerns the expansion of emergent macrophytes in aquatic-terrestrial ecotones. Hence, this review highlights the impact of climatic changes towards aquatic macrophytes and their possible environmental implications.

  11. Epidemiology and control of spinach downy mildew in coastal California

    Science.gov (United States)

    The most serious threat to global fresh market spinach production is spinach downy mildew, caused by the obligate biotrophic pathogen, Peronospora effusa. Downy mildew causes yellow chlorotic lesions on spinach leaf tissue, often accompanied by abundant sporulation on the undersides of leaves. Very ...

  12. Community photosynthesis of aquatic macrophytes

    DEFF Research Database (Denmark)

    Binzer, T.; Sand-Jensen, K.; Middelboe, A. L.

    2006-01-01

    We compared 190 photosynthesis-irradiance (P-E) experiments with single- and multispecies communities of macroalgae and vascular plants from freshwater and marine habitats. We found a typical hyperbolic P-E relation in all communities and no sign of photosaturation or photoinhibition of photosynt......We compared 190 photosynthesis-irradiance (P-E) experiments with single- and multispecies communities of macroalgae and vascular plants from freshwater and marine habitats. We found a typical hyperbolic P-E relation in all communities and no sign of photosaturation or photoinhibition...... of photosynthesis at the highest irradiances of about 2,000 mmol m22 s21. Macrophyte communities displayed much higher maximum gross production (GPmax), respiration, and light compensation point than separate phytoelements because of the multilayered structure and extensive self-shading in the communities, whereas...

  13. Community photosynthesis of aquatic macrophytes

    DEFF Research Database (Denmark)

    Binzer, T.; Sand-Jensen, K.; Middelboe, A. L.

    2006-01-01

    We compared 190 photosynthesis-irradiance (P-E) experiments with single- and multispecies communities of macroalgae and vascular plants from freshwater and marine habitats. We found a typical hyperbolic P-E relation in all communities and no sign of photosaturation or photoinhibition of photosynt......We compared 190 photosynthesis-irradiance (P-E) experiments with single- and multispecies communities of macroalgae and vascular plants from freshwater and marine habitats. We found a typical hyperbolic P-E relation in all communities and no sign of photosaturation or photoinhibition...... of photosynthesis at the highest irradiances of about 2,000 mmol m22 s21. Macrophyte communities displayed much higher maximum gross production (GPmax), respiration, and light compensation point than separate phytoelements because of the multilayered structure and extensive self-shading in the communities, whereas...

  14. Responses of spinach to salinity and nutrient deficiency in growth, physiology and nutritional value

    Science.gov (United States)

    Salinity and nutrient depleted soil are major constraints to crop production, especially for vegetable crops. The effects of salinity and nutrient deficiency on spinach were evaluated in sand cultures under greenhouse conditions. Plants were watered every day with Hoagland nutrition solution, depriv...

  15. Aquatic macrophyte richness in Danish lakes in relation to alkalinity, transparency, and lake area

    DEFF Research Database (Denmark)

    Vestergaard, Ole Skafte; Sand-Jensen, Kaj

    2000-01-01

    alkalinity but 12.3 in lakes of high alkalinity due to a greater occurrence of the species-rich group of elodeids. Mean species richness per lake also increased significantly with increasing Secchi depth. No significant relationship between species richness and lake surface area was observed among the entire...... group of lakes or a subset of eutrophic lakes, as the growth of submerged macrophytes in large lakes may be restricted by wave action in shallow water and light restriction in deep water. In contrast, macrophyte species richness increased with lake surface area in transparent lakes, presumably due...

  16. Factors involved in spatiotemporal dynamics of submerged macrophytes in a Portuguese coastal lagoon under Mediterranean climate

    Science.gov (United States)

    Antunes, Cristina; Correia, Otília; Marques da Silva, Jorge; Cruces, Anabela; Freitas, Maria da Conceição; Branquinho, Cristina

    2012-09-01

    Submerged macrophytes are key in coastal ecosystems, with important structural and functional roles. Thus, the characterization of the submerged aquatic vegetation dynamics is of prime importance for assessing the ecological status of coastal ecosystems. The main aim of this study was to assess the spatial and temporal dynamics of submerged macrophytes biomass in a temporarily open coastal lagoon in Melides, Portugal, and to evaluate the physiological performance of the dominant macrophyte in the system. This lagoon is subject to several disturbances such as the dramatic changes in water physicochemical parameters over time, since temporarily opens to the sea occur renewing the lagoon water content. Moreover it is under a Mediterranean climate with the inherently high temporal variability of precipitation and high temperatures during the summer. Our study shows that the submerged macrophyte community was dominated initially by Ruppia cirrhosa and then replaced by Potamogeton pectinatus, which showed a marked temporal and spatial pattern with extremely high values of biomass in August and in the inner part of the lagoon. The spatial and temporal biomass patterns in submerged macrophytes were mainly related with water level, salinity, pH and transparency. The physiological performance of R. cirrhosa was lower when there was greater biomass accumulation and in extremely shallow waters, whereas physiological vigor seemed to be greater at moderate temperatures and in areas with higher salinity and dissolved oxygen. The data from this study can be used to predict submerged biomass macrophyte responses to extreme water quality changes and to feed a decision support system for the best period to promote the seasonal artificial breaching of the sandy barrier.

  17. Simple relationships to predict attributes of fish assemblages in patches of submerged macrophytes

    Directory of Open Access Journals (Sweden)

    Fernando Mayer Pelicice

    Full Text Available Submerged macrophytes play an important role in structuring habitats and, therefore, in determining patterns of aquatic biodiversity. Because these plants are widespread in shallow areas of many Neotropical reservoirs, the present work investigated if variables related to habitat structure, measured in patches of submerged macrophytes (Egeria densa and E. najas, can be used to predict fish assemblage attributes (fish density and species richness. Based on patch characteristics at fine spatial extents (macrophyte patches within reservoir arms, we considered plant biomass, volume and proportional volume (i.e. percentage of macrophyte volume in the water column as potential predictors. Fish and macrophytes were sampled with a 1-m² throw trap in littoral habitats of Rosana Reservoir, Paranapanema River, and simple correlation analyses were performed. Fish richness and abundance were highly correlated with all variables (R = 0.53 to 0.90, a relationship consistently observed in all sites. When compared to biomass, plant volume and proportional volume did not yield stronger correlations. We observed stronger correlations when E. densa and E. najas patches were analyzed separately (mono-specificity, probably because particular effects of each macrophyte on habitat structuring were removed (e.g. unnoticed morphological differences or unknown effects on habitat quality. The high R values observed in all pairwise relationships are uncommon in ecological studies, highlighting the predictive potential of variables related to habitat structure. These results suggest that, at small spatial extents, macrophyte biomass may represent an interesting predictor of fish density and richness in reservoirs with extensive colonization of submerged plants.

  18. Retrospective assessment of macrophytic communities in southern Lake Garda (Italy from in situ and MIVIS (Multispectral Infrared and Visible Imaging Spectrometer data

    Directory of Open Access Journals (Sweden)

    Claudia Giardino

    2012-01-01

    Full Text Available In situ and hyperspectral MIVIS (Multispectral Infrared and Visible Imaging Spectrometer images acquired over a period of 13 years are used to assess changes in macrophyte colonization patterns in the coastal zones of the Sirmione Peninsula in the southern part of Lake Garda (Italy. In situ data (abundance, cover density and diversity of macrophyte communities and MIVIS-derived maps of colonized substrates are analyzed by considering the variability of the main hydrological and physicochemical variables in order to indicate the main factors that explain the spatiotemporal variability of macrophyte communities. The results show a considerable modification in terms of macrophyte structural complexity and colonized areas. Almost 98% of macrophyte meadows (in particular communities with a density of over 70% are lost and subsequently replaced by moderate to extremely rare communities with density from 10% to 40%. Well-established submerged macrophytes are replaced by de-structured communities characterized by moderate to scarce density: on average lower than 30%. The study indicates that macrophyte distribution along the littoral zone of the Sirmione Peninsula is certainly linked to water transparency and water level fluctuation. The results also indicate that the worsening of eutrophication may be associated with the gradual disappearance of macrophyte meadows, but may also be accelerated by herbivorous aquatic birds grazing there. Lastly, the increasing frequency and number of catamaran tours could be considered a threat for the stability of these valuable communities.

  19. Suppression subtractive hybridization (SSH)-based method for estimating Cd-induced differences in gene expression at cultivar level and identification of genes induced by Cd in two water spinach cultivars.

    Science.gov (United States)

    Huang, Baifei; Xin, Junliang; Yang, Zhongyi; Zhou, Yihui; Yuan, Jiangang; Gong, Yulian

    2009-10-14

    The abilities to accumulate cadmium (Cd) are different among cultivars (cv.) in many species. The characteristic of Cd concentration among cultivars is heritable and is probably controlled by genes, but rather limited information about the relevant genes in vegetable crops has been published. In the present study, a suppression subtractive hybridization (SSH) approach was used to identify genes induced by Cd in two water spinach (an important vegetable in southern China) cultivars that differ in Cd accumulation in their edible parts. The two cultivars were cv. Qiangkunqinggu (QK), a low Cd accumulative cultivar and cv. Taiwan 308 (TW), a high Cd accumulative cultivar. In the construction of QK and TW libraries, the plants without Cd treatment were taken as drivers and the plants exposed to 6 mg L(-1) Cd for 24 h as testers. Four hundred clones were sequenced, and 164 nonrepeated sequences (112 from the QK library and 52 from the TW library) were assigned to being functional genes or proteins. A tremendous difference in Cd-induced gene expressions between the two libraries was observed. In the QK library, genes implicated in disease/defense comprised one of the largest sets (20.6%), whereas the proportion was only 8.8% in the TW library. An MT3 gene (Q5), a wound inductive gene (Q22), an antioxidation relevant gene (Q34), a lectin gene (Q45), an f-box family protein gene (Q319), a 20S proteasome subunit gene (T17), a multidrug resistance associated protein gene (T156), and a cationic amino acid transporter gene (T218) were selected to compare semiquantitatively their expression between cv. QK and cv. TW using the RT-PCR method, and obvious differences were detected. The relationships between the identified differences in the expressions of the genes and the Cd accumulation of the two cultivars were discussed, and it was concluded that the SSH approach is useful for finding the difference in expression of Cd-induced gene even at the cultivar level and is applicable

  20. Determination of Algae and Macrophyte Species Distribution in Three Wastewater Stabilization Ponds Using Metagenomics Analysis

    Directory of Open Access Journals (Sweden)

    Jack Wallace

    2015-06-01

    Full Text Available This study involved the evaluation of algae and macrophyte species distributions in three wastewater stabilization ponds (WSPs at a wastewater treatment plant in Ontario, Canada, which has experienced high pH levels at the final effluent and excessive algae growth during the summer since 2003. From samples collected from the system, the relative abundances of specific algae and aquatic plant (macrophyte taxa were assessed and correlated to water chemistry data. A strong shift from the dominance of green algae, chlorophyceae, in WSP#2, to the dominance of aquatic macrophytes, embryophyta, in WSP#4, was observed and corresponded to field observations. Correlation of the abundances to nutrient parameters suggested that the macronutrient rich conditions in WSP#2 allowed floating green algae to proliferate against macrophytes. In WSP#1 and WSP#4, macrophytes competed against algae and thrived, due to their adaptability to lower nutrient conditions. The pH increases occurred primarily in WSP#2 and were not buffered or reduced in WSP#1 and WSP#4. Two alternatives strategies for pH control were recommended for the system: decreasing algae growth in WSP#2 through duckweed seeding or macronutrient loading reduction; or designing and implementing a constructed wetland (CW in WSP#4 with soil and vegetation to buffer pH prior to release.

  1. Integration of geographic information systems and logistic multiple regression for aquatic macrophyte modeling

    Energy Technology Data Exchange (ETDEWEB)

    Narumalani, S. [Nebraska Univ., Lincoln, NE (United States). Dept. of Geography; Jensen, J.R.; Althausen, J.D.; Burkhalter, S. [South Carolina Univ., Columbia, SC (United States). Dept. of Geography; Mackey, H.E. Jr. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1994-06-01

    Since aquatic macrophytes have an important influence on the physical and chemical processes of an ecosystem while simultaneously affecting human activity, it is imperative that they be inventoried and managed wisely. However, mapping wetlands can be a major challenge because they are found in diverse geographic areas ranging from small tributary streams, to shrub or scrub and marsh communities, to open water lacustrian environments. In addition, the type and spatial distribution of wetlands can change dramatically from season to season, especially when nonpersistent species are present. This research, focuses on developing a model for predicting the future growth and distribution of aquatic macrophytes. This model will use a geographic information system (GIS) to analyze some of the biophysical variables that affect aquatic macrophyte growth and distribution. The data will provide scientists information on the future spatial growth and distribution of aquatic macrophytes. This study focuses on the Savannah River Site Par Pond (1,000 ha) and L Lake (400 ha) these are two cooling ponds that have received thermal effluent from nuclear reactor operations. Par Pond was constructed in 1958, and natural invasion of wetland has occurred over its 35-year history, with much of the shoreline having developed extensive beds of persistent and non-persistent aquatic macrophytes.

  2. Analysis of Satellite and Airborne Imagery for Detection of Water Hyacinth and Other Invasive Floating Macrophytes and Tracking of Aquatic Weed Control Efficacy

    Science.gov (United States)

    Potter, Christopher

    2016-01-01

    Waterways of the Sacramento San Joaquin Delta have recently become infested with invasive aquatic weeds such as floating water hyacinth (Eichhoria crassipes) and water primrose (Ludwigia peploides). These invasive plants cause many negative impacts, including, but not limited to: the blocking of waterways for commercial shipping and boating; clogging of irrigation screens, pumps and canals; and degradation of biological habitat through shading. Zhang et al. (1997, Ecological Applications, 7(3), 1039-1053) used NASA Landsat satellite imagery together with field calibration measurements to map physical and biological processes within marshlands of the San Francisco Bay. Live green biomass (LGB) and related variables were correlated with a simple vegetation index ratio of red and near infra-red bands from Landsat images. More recently, the percent (water area) cover of water hyacinth plotted against estimated LGB of emergent aquatic vegetation in the Delta from September 2014 Landsat imagery showed an 80 percent overall accuracy. For the past two years, we have partnered with the U. S. Department of Agriculture (USDA) and the Department of Plant Sciences, University of California at Davis to conduct new validation surveys of water hyacinth and water primrose coverage and LGB in Delta waterways. A plan is underway to transfer decision support tools developed at NASA's Ames Research Center based on Landsat satellite images to improve Delta-wide integrated management of floating aquatic weeds, while reducing chemical control costs. The main end-user for this application project will be the Division of Boating and Waterways (DBW) of the California Department of Parks and Recreation, who has the responsibility for chemical control of water hyacinth in the Delta.

  3. New Submerged Macrophytes Revegetation Method and Its Impacts on Water Environment%新型沉水植物种植方法及其对水环境的影响研究

    Institute of Scientific and Technical Information of China (English)

    高翔; 周维成; 彭成荣; 李双双; 张凤格; 王高鸿

    2015-01-01

    Restoring submerged macrophytes artificially is one of the effective measures to recover the contaminated water eco-system.In this study a new revegetation method by employing vallisneria natans was established, in which mud or clay used as package material in traditional sinking method was replaced by agar gel.At the same time we monitored the growth of vallisneria natans and water quality of two different planting methods.The research results showed that under the agar gel-sinking method the survival rate of vallisneria natans reached 100% and other measuring parameters such as biomass, optimal/maximal photo-chemical efficiency of PSⅡin the dark, and the growth of F1 plants also have excellent performance.Meanwhile during the exper-iment time with the sharp drop of CODMn value, total nitrogen content, total phosphorus content and discernable increase of dis-solved oxygen value, oxidation-reduction potential value water quality improved significantly.%人工快速种植沉水植物是受污染水体生态修复与沉水植物重建的有效措施之一.研究采用琼脂凝胶取代传统抛栽法的软泥或粘土作为外裹物对苦草进行人工种植,并与传统插栽法进行了对比研究,结果表明:(1)新型琼脂抛栽法种植的苦草成活率达到100%,生物量、光合效率、子株生长情况等指标都表现优良;(2)新型琼脂抛栽法种植苦草过程中水体的CODMn值、TN含量、TP含量都现大幅下降,DO值、ORP值都明显上升,水体水质明显改善.

  4. Macrophytes of the Grlište reservoir (Serbia: Fifteen years after its establishment

    Directory of Open Access Journals (Sweden)

    Stanković Ž.

    2009-01-01

    Full Text Available A large number of macrophytes, often in dense populations, have developed on the Grlište Reservoir, Serbia over a period of 15 years. Fast development of vegetation is a consequence of anthropogenic impact in lake management. The methodology used in this research covered 100% of the water body, including all areas with or without aquatic plants. The results indicate that plant communities are still in the early phase of development. This leaves space for future development of competitor macrophyte species (Najas marina, Eleocharis palustris, Typha latifolia, Typha angustifolia, Phragmites australis, etc. capable of endangering stability of the lake, which will tend toward eutrophication.

  5. Functional diversity of macrophyte communities within and between Pyrenean lakes

    Directory of Open Access Journals (Sweden)

    Enric BALLESTEROS

    2009-02-01

    Full Text Available Submersed vegetation is a common feature in about 70% Pyrenean high mountain (>1500 m a.s.l. lakes. Isoetids and soft-water elodeids are common elements of this underwater flora and can form distinct vegetation units (i.e. patches of vegetation dominated by different species within complex mosaics of vegetation in shallow waters (<7 m. Since isoetids exert a strong influence on sediment biogeochemistry due to high radial oxygen loss, we examined the small scale characteristics of the lake environment (water and sediment associated to vegetation patches in order to ascertain potential functional differences among them. To do so, we characterised the species composition and biomass of the main vegetation units from 11 lakes, defined plant communities based on biomass data, and then related each community with sediment properties (redox and dissolved nutrient concentration in the pore water and water nutrient concentration within plant canopy. We also characterised lake water and sediment in areas without vegetation as a reference. A total of twenty-one vegetation units were identified, ranging from one to five per lake. A cluster analysis on biomass species composition suggested seven different macrophyte communities that were named after the most dominant species: Nitella sp., Potamogeton praelongus, Myriophyllum alterniflorum, Sparganium angustifolium, Isoetes echinospora, Isoetes lacustris and Carex rostrata. Coupling between macrophyte communities and their immediate environment (overlying water and sediment was manifested mainly as variation in sediment redox conditions and the dominant form of inorganic nitrogen in pore-water. These effects depended on the specific composition of the community, and on the allocation between above- and belowground biomass, and could be predicted with a model relating the average and standard deviation of sediment redox potential from 0 down to -20 cm, across macrophyte communities. Differences in pore-water

  6. A meta-analysis of water quality and aquatic macrophyte responses in 18 lakes treated with lanthanum modified bentonite (Phoslock®)

    NARCIS (Netherlands)

    Spears, Bryan M.; Mackay, Eleanor B.; Yasseri, Said; Gunn, Iain D.M.; Waters, Kate E.; Andrews, Christopher; Cole, Stephanie; De Ville, Mitzi; Kelly, Andrea; Meis, Sebastian; Moore, Alanna L.; Nürnberg, Gertrud K.; van Oosterhout, Frank; Pitt, Jo-Anne; Madgwick, Genevieve; Woods, Helen J.; Lürling, Miquel

    2016-01-01

    Abstract Lanthanum (La) modified bentonite is being increasingly used as a geo-engineering tool for the control of phosphorus (P) release from lake bed sediments to overlying waters. However, little is known about its effectiveness in controlling P across a wide range of lake conditions or of its po

  7. The importance of catchment vegetation for alkalinity, phosphorus burial and macrophytes as revealed by a recent paleolimnological study in a soft water lake

    DEFF Research Database (Denmark)

    Klamt, Anna-Marie; Jensen, Henning S.; Mortensen, Morten Fischer

    2017-01-01

    to mainly coniferous forest) occurring during the last ca. 200years were reflected in the sediments of a soft water lake and how these changes influenced the lake ecosystem. Pollen, macrofossils, metals, different phosphorus (P) forms, organic matter, carbon and nitrogen contents were determined in short...

  8. The importance of catchment vegetation alkalinity, phosphorus burial and macrophytes as revealed by a recent paleolimnological study in a soft water lake

    DEFF Research Database (Denmark)

    Klamt, Anna-Maria; Jensen, Henning S.; Mortensen, Morten Fischer

    2016-01-01

    coniferous forest) occurring during the last ca. 200 years were reflected in the sediments of a soft water lake and how these changes influenced the lake ecosystem. Pollen, macrofossils, metals, different phosphorus (P) forms, organic matter, carbon and nitrogen contents were determined in short sediment...

  9. Impact of submerged macrophytes including charophytes on phyto- and zooplankton communities: allelopathy versus other mechanisms

    NARCIS (Netherlands)

    Van Donk, E.; Van de Bund, W.J.

    2002-01-01

    Submerged macrophytes are crucial for the stabilization of the clear water state in shallow, mesotrophic and eutrophic lakes. Especially, charophytes often play an important role because they are typically rapid colonizers and because charophyte meadows are believed to have a particularly strong pos

  10. Mowing Submerged Macrophytes in Shallow Lakes with Alternative Stable States: Battling the Good Guys?

    NARCIS (Netherlands)

    Kuiper, Jan J.; Verhofstad, Michiel J. J. M.; Louwers, Evelien; Bakker, Elisabeth S.; Brederveld, Robert J.; van Gerven, Luuk P. A.; Janssen, Annette B. G.; de Klein, Jeroen J. M.; Mooij, Wolf M.

    2017-01-01

    Submerged macrophytes play an important role in maintaining good water quality in shallow lakes. Yet extensive stands easily interfere with various services provided by these lakes, and harvesting is increasingly applied as a management measure. Because shallow lakes may possess alternative stable

  11. Effects of contrasting omnivorous fish on submerged macrophyte biomass in temperate lakes: a mesocosm experiment

    NARCIS (Netherlands)

    Dorenbosch, M.; Bakker, E.S.

    2012-01-01

    1.Freshwater fish can affect aquatic vegetation directly by consuming macrophytes or indirectly by changing water quality. However, most fish in the temperate climate zone have an omnivorous diet. The impact of fish as aquatic herbivores in temperate climates therefore remains unclear and depends on

  12. Clonal variation in the thermal response of the submerged aquatic macrophyte Potamogeton pectinatus

    NARCIS (Netherlands)

    Pilon, J.; Santamaria, L.

    2002-01-01

    Broadly distributed plants have to cope with dramatic differences across latitude in the prevailing environmental temperature. We investigated the effect of water temperature on plant morphology, biomass accumulation and oxygen-exchange for five clones of the submerged aquatic macrophyte Potamogeton

  13. Effects of contrasting omnivorous fish on submerged macrophyte biomass in temperate lakes: a mesocosm experiment

    NARCIS (Netherlands)

    Dorenbosch, M.; Bakker, E.S.

    2012-01-01

    1.Freshwater fish can affect aquatic vegetation directly by consuming macrophytes or indirectly by changing water quality. However, most fish in the temperate climate zone have an omnivorous diet. The impact of fish as aquatic herbivores in temperate climates therefore remains unclear and depends on

  14. Impact of submerged macrophytes including charophytes on phyto- and zooplankton communities: allelopathy versus other mechanisms

    NARCIS (Netherlands)

    Van Donk, E.; Van de Bund, W.J.

    2002-01-01

    Submerged macrophytes are crucial for the stabilization of the clear water state in shallow, mesotrophic and eutrophic lakes. Especially, charophytes often play an important role because they are typically rapid colonizers and because charophyte meadows are believed to have a particularly strong

  15. A preliminary study of the feasibility of detecting the floating macrophytes by means of digital processing of MSS/LANDSAT data

    Science.gov (United States)

    Valeriano, D. D. (Principal Investigator)

    1984-01-01

    The feasibility of mapping the floating macrophytes (mostly water hyacinth: (Eichornia crassipes) in the Salto Grande reservoir in Americana, SP, by means of digital processing of MSS/LANDSAT data is described. The area occupied by the macrophytes was extracted by exclusion after the merging of two thematic masks representing the area of vegetation free water surface. One of the masks was obtained from a date when the vegetal cover is insignificant, while the other represents a large infestation episode. The utilization of digital processing of MSS/LANDSAT data techniques for the documentation of macrophytes infestation is feasible only when the phenomenon occurs in large areas.

  16. Efficiency of aquatic macrophytes to treat Nile tilapia pond effluents

    Directory of Open Access Journals (Sweden)

    Henry-Silva Gustavo Gonzaga

    2006-01-01

    Full Text Available The effluents from fish farming can increase the quantity of suspended solids and promote the enrichment of nitrogen and phosphorus in aquatic ecosystems. In this context, the aim of this work was to evaluate the efficiency of three species of floating aquatic macrophytes (Eichhornia crassipes, Pistia stratiotes and Salvinia molesta to treat effluents from Nile tilapia culture ponds. The effluent originated from a 1,000-m² pond stocked with 2,000 male Nile tilapia Oreochromis niloticus. The treatment systems consisted of 12 experimental tanks, three tanks for each macrophyte species, and three control tanks (without plants. Water samples were collected from the: (i fish pond source water, (ii effluent from fish pond and (iii effluents from the treatment tanks. The following water variables were evaluated: turbidity, total and dissolved nitrogen, ammoniacal-N, nitrate-N, nitrite-N, total phosphorus and dissolved phosphorus. E. crassipes and P. stratiotes were more efficient in total phosphorus removal (82.0% and 83.3%, respectively and total nitrogen removal (46.1% and 43.9%, respectively than the S. molesta (72.1% total phosphorus and 42.7% total nitrogen and the control (50.3% total phosphorus and 22.8% total nitrogen, indicating that the treated effluents may be reused in the aquaculture activity.

  17. Cadmium and chromium effects on seed germination and root elongation in lettuce, spinach and Swiss chard

    Directory of Open Access Journals (Sweden)

    Oscar V Bautista

    2013-04-01

    Full Text Available The La Ramada district and the Bogota River are the principal water resources used for horticultural crop production on the Bogota Plateau, which contain channel pollutant materials, including heavy metals due to domestic and industrial activities on the Plateau. These materials have effects on crop production in this zone. The present research, under laboratory conditions, aimed to evaluate the effect of three concentrations (25, 35 and 50 µM L-1 of cadmium (Cd and chromium (Cr on imbibition, seed germination and root elongation in lettuce (Lactuca sativa var. Batavia, Swiss chard (Beta vulgaris var. cicla "White Ribbed", and spinach (Spinacia oleracea Hib. 424, three species widely cultivated on the Plateau. The three species used for evaluation showed a differential susceptibility response to Cd and Cr. In lettuce, fresh weight increase (imbibition was lower with all Cd concentrations at the last day of observation and at 25 µM L-1 of Cd in Swiss chard. Cadmium reduced seed germination by up to 46%, 97% and 8% in Swiss chard, lettuce and spinach, respectively. Also, root elongation decreased in Cd treatments by up to 57%, 89% and 56%, for Swiss chard, lettuce and spinach, respectively. Chromium, which showed fewer negative effects, decreased germination by up to 29% in Swiss chard, 6% in lettuce and 34% in spinach, as compared to the control

  18. Mechanism of LaCl3 on Increasing Photosystem Ⅱ Activity of Spinach

    Institute of Scientific and Technical Information of China (English)

    HONG Fa-Shui; WANG Ling; TAO Ye

    2005-01-01

    The effect of LaCl3 on the K3Fe(CN)6 (FeCy) reduction rate and the oxygen-evolving rate of PSⅡ particles of spinach, and the spectral characterization of the D1/D2/Cytb559 of a PSⅡ reaction center complex consisting of three polypeptides from spinach were studied. The experimental results showed that LaCl3 could significantly accelerate the transformation from light energy to electric energy, the electron transport, water photolysis and oxygen evolution of PSⅡ of spinach, which was related to the spectral characterization of the D1/D2/Cytb559 complex.Soret band and Q band of Chl-a of UV-vis spectrum of D1/D2/Cytb559 complex were blue shifted, and the fluorescence emission peak was blue shifted in LaCl3 treated spinach compared with that in the control. The EXAFS (extended X-ray absorption fine structure spectroscopy) revealed that La3+ was coordinated with 8 nitrogen or oxygen atoms in the first coordination shell with La-N or La-O bond length of 0.254 nm, and with 6 nitrogen or oxygenatoms in the second coordination shell with La-N or La-O bond length of 0.321 nm in the D1/D2/Cytb559 complex. The CD suggested that the secondary structure of D1/D2/Cytb559 complex have been little affected by the treatment of LaCl3.

  19. Evaluation of a combined macrophyte-epiphyte bioassay for assessing nutrient enrichment in the Portneuf River, Idaho, USA.

    Science.gov (United States)

    Ray, Andrew M; Mebane, Christopher A; Raben, Flint; Irvine, Kathryn M; Marcarelli, Amy M

    2014-07-01

    We describe and evaluate a laboratory bioassay that uses Lemna minor L. and attached epiphytes to characterize the status of ambient and nutrient-enriched water from the Portneuf River, Idaho. Specifically, we measured morphological (number of fronds, longest surface axis, and root length) and population-level (number of plants and dry mass) responses of L. minor and community-level (ash-free dry mass [AFDM] and chlorophyll a [Chl a]) responses of epiphytes to nutrient enrichment. Overall, measures of macrophyte biomass and abundance increased with increasing concentrations of dissolved phosphorus (P) and responded more predictably to nutrient enrichment than morphological measures. Epiphyte AFDM and Chl a were also greatest in P-enriched water; enrichments of N alone produced no measurable epiphytic response. The epiphyte biomass response did not directly mirror macrophyte biomass responses, illustrating the value of a combined macrophyte-epiphyte assay to more fully evaluate nutrient management strategies. Finally, the most P-enriched waters not only supported greater standing stocks of macrophyte and epiphytes but also had significantly higher water column dissolved oxygen and dissolved organic carbon concentrations and a lower pH. Advantages of this macrophyte-epiphyte bioassay over more traditional single-species assays include the use of a more realistic level of biological organization, a relatively short assay schedule (~10 days), and the inclusion of multiple biological response and water-quality measures.

  20. Effects of interspecific competition on the growth of macrophytes and nutrient removal in constructed wetlands: A comparative assessment of free water surface and horizontal subsurface flow systems.

    Science.gov (United States)

    Zheng, Yucong; Wang, Xiaochang; Dzakpasu, Mawuli; Zhao, Yaqian; Ngo, Huu Hao; Guo, Wenshan; Ge, Yuan; Xiong, Jiaqing

    2016-05-01

    The outcome of competition between adjoining interspecific colonies of Phragmites and Typha in two large field pilot-scale free water surface (FWS) and subsurface flow (SSF) CWs is evaluated. According to findings, the effect of interspecific competition was notable for Phragmites australis, whereby it showed the highest growth performance in both FWS and SSF wetland. In a mixed-culture, P. australis demonstrates superiority in terms of competitive interactions for space between plants. Furthermore, the interspecific competition among planted species seemed to cause different ecological responses of plant species in the two CWs. For example, while relatively high density and shoot height determined the high aboveground dry weight of P. australis in the FWS wetland, this association was not evident in the SSF. Additionally, while plants nutrients uptake accounts for a higher proportion of the nitrogen removal in FWS, that in the SSF accounts for a higher proportion of the phosphorous removal. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Residue Determination of Dimethoate in Leafy Vegetables (Spinach) using RPHPLC

    OpenAIRE

    J. BAGYALAKSHMI,; Kavitha, G.; Ravi, T. K.

    2011-01-01

    Residues of Dimethoate present in locally available Spinach varities - Palak : Beta maritima, Vendhai : Trigonella foenumgraecum, Thandan : Amarnathus mangostanus - green and red were determined. The quantification was carried out using RP-HPLC for unprocessed (fresh) and processed (washed, washed and cooked) Spinach samples. The extraction was carried out with benzene. It was found that unprocessedDimethoate Spinach contains dimethoate residue which is slightly more than the tolerance limit,...

  2. Effect of Hydrologic Alteration on the Community Succession of Macrophytes at Xiangyang Site, Hanjiang River, China

    Directory of Open Access Journals (Sweden)

    Na Yang

    2017-01-01

    Full Text Available With the intensification of human activities over the past three decades in China, adverse effects on river ecosystem become more serious especially in the Hanjiang River. Xiangyang site is an important spawn ground for four domestic fishes in the downstream region of Hanjiang River. Based on the field survey results of macrophytes during 1997–2000 and 2013-2014, community succession of aquatic macrophytes at Xiangyang site was evaluated and discussed. Two-key ecologic-related hydrologic characteristics, flow regime and water level, were identified as the main influence factors. The EFC (environmental flow components parameters were adopted to evaluate the alteration of flow regimes at Xiangyang site during 1941–2013. Evaluation results demonstrate a highly altered flow process after being regulated by reservoir. The flow patterns tend to be an attenuation process with no large floods occurring but a higher monthly low flow. Furthermore, the water level decreased and fluctuation reduced after the dam was built, which caused the decrease of biomass but favored the submerged macrophytes during 1995–2009. However, with the water level increasing after 2010 and gently fluctuating, due to uplift by the hydraulic projects downstream as well as the flow attenuation, the dominant position of submerged macrophytes will be weakened.

  3. Effect of Hydrologic Alteration on the Community Succession of Macrophytes at Xiangyang Site, Hanjiang River, China

    Science.gov (United States)

    Duan, Kai

    2017-01-01

    With the intensification of human activities over the past three decades in China, adverse effects on river ecosystem become more serious especially in the Hanjiang River. Xiangyang site is an important spawn ground for four domestic fishes in the downstream region of Hanjiang River. Based on the field survey results of macrophytes during 1997–2000 and 2013-2014, community succession of aquatic macrophytes at Xiangyang site was evaluated and discussed. Two-key ecologic-related hydrologic characteristics, flow regime and water level, were identified as the main influence factors. The EFC (environmental flow components) parameters were adopted to evaluate the alteration of flow regimes at Xiangyang site during 1941–2013. Evaluation results demonstrate a highly altered flow process after being regulated by reservoir. The flow patterns tend to be an attenuation process with no large floods occurring but a higher monthly low flow. Furthermore, the water level decreased and fluctuation reduced after the dam was built, which caused the decrease of biomass but favored the submerged macrophytes during 1995–2009. However, with the water level increasing after 2010 and gently fluctuating, due to uplift by the hydraulic projects downstream as well as the flow attenuation, the dominant position of submerged macrophytes will be weakened. PMID:28127493

  4. A Spinach molecular beacon triggered by strand displacement.

    Science.gov (United States)

    Bhadra, Sanchita; Ellington, Andrew D

    2014-08-01

    We have re-engineered the fluorescent RNA aptamer Spinach to be activated in a sequence-dependent manner. The original Spinach aptamer was extended at its 5'- and 3'-ends to create Spinach.ST, which is predicted to fold into an inactive conformation and thus prevent association with the small molecule fluorophore DFHBI. Hybridization of a specific trigger oligonucleotide to a designed toehold leads to toehold-initiated strand displacement and refolds Spinach into the active, fluorophore-binding conformation. Spinach.ST not only specifically detects its target oligonucleotide but can discriminate readily against single-nucleotide mismatches. RNA amplicons produced during nucleic acid sequence-based amplification (NASBA) of DNA or RNA targets could be specifically detected and reported in real-time by conformational activation of Spinach.ST generated by in vitro transcription. In order to adapt any target sequence to detection by a Spinach reporter we used a primer design technique that brings together otherwise distal toehold sequences via hairpin formation. The same techniques could potentially be used to adapt common Spinach reporters to non-nucleic acid analytes, rather than by making fusions between aptamers and Spinach.

  5. Abscisic Acid accumulation in spinach leaf slices in the presence of penetrating and nonpenetrating solutes.

    Science.gov (United States)

    Creelman, R A; Zeevaart, J A

    1985-01-01

    Abscisic acid (ABA) accumulated in detached, wilted leaves of spinach (Spinacia oleracea L. cv Savoy Hybrid 612) and reached a maximum level within 3 to 4 hours. The increase in ABA over that found in detached turgid leaves was approximately 10-fold. The effects of water stress could be mimicked by the use of thin slices of spinach leaves incubated in the presence of 0.6 molar mannitol, a compound which causes plasmolysis (loss of turgor). About equal amounts of ABA were found both in the leaf slices and in detached leaves, whereas 2 to 4 times more ABA accumulated in the medium than in the slices. When spinach leaf slices were incubated with ethylene glycol, a compound which rapidly penetrates the cell membrane causing a decrease in the osmotic potential of the tissue and only transient loss of turgor, no ABA accumulated. Ethylene glycol was not inhibitory with respect to ABA accumulation. Spinach leaf slices incubated in both ethylene glycol and mannitol had ABA levels similar to those found when slices were incubated with mannitol alone. Increases similar to those found with mannitol also occurred when Aquacide III, a highly purified form of polyethylene glycol, was used. Aquacide III causes cytorrhysis, a situation similar to that found in wilted leaves. Thus, it appears that loss of turgor is essential for ABA accumulation.When spinach leaf slices were incubated with solutes which are supposed to disturb membrane integrity (KHSO(3), 2-propanol, or KCl) no increase in ABA was observed. These data indicate that, with respect to the accumulation of ABA, mannitol caused a physical stress (loss of turgor) rather than a chemical stress (membrane damage).

  6. Interaction between Silver Nanoparticles and Spinach Leaf

    Science.gov (United States)

    Tian, Y.; Li, H.; Zhang, Y.; Riser, E.; He, S.; Zhang, W.

    2013-12-01

    Interactions of engineered nanoparticles (ENPs) with plant surfaces are critical to assessing the bioavailability of ENPs to edible plants and to further evaluating impacts of ENPs on ecological health and food safety. Silver nanoparticles (i.e., nanoAg) could enter the agroecosystems either as an active ingredient in pesticides or from other industrial and consumer applications. Thus, in the events of pesticide application, rainfall, and irrigation, vegetable leaves could become in contact and then interact with nanoAg. The present study was to assess whether the interaction of nanoAg with spinach leaves can be described by classical sorption models and to what extent it depends on and varies with dispersion methods, environmental temperature, and ion release. We investigated the stability and ion release of nanoAg dispersed by sodium dodecyl sulfate (SDS, 1%) and humic acid (HA, 10 mg C/L) solutions, as well as sorption and desorption of nanoAg on and from the fresh spinach leaf. Results showed SDS-nanoAg released about 2%-8% more Ag ion than HA-nanoAg. The sorption of Ag ion, described by the Freundlich model in the initial concentration range of 0.6-50 mg/L, was 2-4 times higher than that of nanoAg. The sorption of nanoAg on spinach leaf can be fitted by the Langmuir model, and the maximum sorption amount of HA-nanoAg and SDS-nanoAg was 0.21 and 0.41 mg/g, respectively. The higher sorption of SDS-nanoAg relative to that of HA-nanoAg could be partially resulted from the higher release of Ag ion from the former. The maximum desorption amount of HA-nanoAg and SDS-nanoAg in 1% SDS solution was 0.08 and 0.10 mg/g, respectively. NanoAg attachment on and its penetration to the spinach leaf was visualized by the Scanning Electron Microscope equipped with an Energy Dispersive Spectrometer (SEM-EDS). It is equally important that the less sorption of nanoAg under low environmental temperature could be partially due to the closure of stomata, as verified by SEM-EDS. Cyto

  7. Simple quantitative analysis of Escherichia coli K-12 internalized in baby spinach using Fourier Transform Infrared spectroscopy.

    Science.gov (United States)

    Wang, Jun; Kim, Kyung Ho; Kim, Sungkyun; Kim, Yong Soo; Li, Qing X; Jun, Soojin

    2010-11-15

    Bacterial contamination continues to be a serious concern for food safety. Although washing fresh produce helps in reducing pathogen levels, pathogen internalization often limits the effectiveness of washing. When pathogens internalize in leafy vegetables, the method of identification and quantitative measurement would be called into question. This study was aimed to use Fourier Transform Infrared (FTIR) spectroscopy integrated with an attenuated total reflectance kit for quantification of Escherichia coli K-12 internalized in baby spinach. The bacteria were inoculated into vascular and intracellar tissues of spinach leaves by syringe injection and the distribution of internalized E. coli K-12 cells was confirmed under scanning electron microscopy (SEM). FTIR measurement following the preparation of bacterial suspension from spinach leaves with high speed pulverizing enabled to detect the absorbance peaks in the amide II region between 1590 and 1490 cm⁻¹ as a fingerprint for the microbes. It was found that the estimated concentrations of E. coli K-12 agreed well with the concentrations determined by plate counting with R² values of 0.98 and 0.97 in peptone water and spinach extracts, respectively. The results demonstrated that FTIR can identify and quantify E. coli K-12 in baby spinach extracts at a limit of detection of approximately 100 CFU/mL in 5 min. The developed method is expected to be suitable for the analysis of pathogenic E. coli strains and other bacterial species in fresh vegetables.

  8. Aquatic macrophytes can be used for wastewater polishing but not for purification in constructed wetlands

    Science.gov (United States)

    Tang, Yingying; Harpenslager, Sarah F.; van Kempen, Monique M. L.; Verbaarschot, Evi J. H.; Loeffen, Laury M. J. M.; Roelofs, Jan G. M.; Smolders, Alfons J. P.; Lamers, Leon P. M.

    2017-02-01

    The sequestration of nutrients from surface waters by aquatic macrophytes and sediments provides an important service to both natural and constructed wetlands. While emergent species take up nutrients from the sediment, submerged and floating macrophytes filter nutrients directly from the surface water, which may be more efficient in constructed wetlands. It remains unclear, however, whether their efficiency is sufficient for wastewater purification and how plant species and nutrient loading affects nutrient distribution over plants, water and sediment. We therefore determined nutrient removal efficiencies of different vegetation (Azolla filiculoides, Ceratophyllum demersum and Myriophyllum spicatum) and sediment types (clay, peaty clay and peat) at three nutrient input rates, in a full factorial, outdoor mesocosm experiment. At low loading (0.43 mg P m-2 d-1), plant uptake was the main pathway (100 %) for phosphorus (P) removal, while sediments showed a net P release. A. filiculoides and M. spicatum showed the highest biomass production and could be harvested regularly for nutrient recycling, whereas C. demersum was outcompeted by spontaneously developing macrophytes and algae. Higher nutrient loading only stimulated A. filiculoides growth. At higher rates ( ≥ 21.4 mg P m-2 d-1), 50-90 % of added P ended up in sediments, with peat sediments becoming more easily saturated. For nitrogen (N), 45-90 % was either taken up by the sediment or lost to the atmosphere at loadings ≥ 62 mg N m-2 d-1. This shows that aquatic macrophytes can indeed function as an efficient nutrient filter but only for low loading rates (polishing) and not for high rates (purification). The outcome of this controlled study not only contributes to our understanding of nutrient dynamics in constructed wetlands but also shows the differential effects of wetland sediment types and plant species. Furthermore, the acquired knowledge may benefit the application of macrophyte harvesting to remove

  9. Plant based phosphorus recovery from wastewater via algae and macrophytes.

    Science.gov (United States)

    Shilton, Andrew N; Powell, Nicola; Guieysse, Benoit

    2012-12-01

    At present, resource recovery by irrigation of wastewater to plants is usually driven by the value of the water resource rather than phosphorus recovery. Expanded irrigation for increased phosphorus recovery may be expected as the scarcity and price of phosphorus increases, but providing the necessary treatment, storage and conveyance comes at significant expense. An alternative to taking the wastewater to the plants is instead to take the plants to the wastewater. Algal ponds and macrophyte wetlands are already in widespread use for wastewater treatment and if harvested, would require less than one-tenth of the area to recover phosphorus compared to terrestrial crops/pastures. This area could be further decreased if the phosphorus content of the macrophytes and algae biomass was tripled from 1% to 3% via luxury uptake. While this and many other opportunities for plant based recovery of phosphorus exist, e.g. offshore cultivation, much of this technology development is still in its infancy. Research that enhances our understanding of how to maximise phosphorus uptake and harvest yields; and further add value to the biomass for reuse would see the recovery of phosphorus via plants become an important solution in the future.

  10. AMEG: the new SETAC advisory group on aquatic macrophyte ecotoxicology.

    Science.gov (United States)

    Arts, Gertie; Davies, Jo; Dobbs, Michael; Ebke, Peter; Hanson, Mark; Hommen, Udo; Knauer, Katja; Loutseti, Stefania; Maltby, Lorraine; Mohr, Silvia; Poovey, Angela; Poulsen, Véronique

    2010-05-01

    Primary producers play critical structural and functional roles in aquatic ecosystems; therefore, it is imperative that the potential risks of toxicants to aquatic plants are adequately assessed in the risk assessment of chemicals. The standard required macrophyte test species is the floating (non-sediment-rooted) duckweed Lemna spp. This macrophyte species might not be representative of all floating, rooted, emergent, and submerged macrophyte species because of differences in the duration and mode of exposure; sensitivity to the specific toxic mode of action of the chemical; and species-specific traits (e.g., duckweed's very short generation time). These topics were addressed during the workshop entitled "Aquatic Macrophyte Risk Assessment for Pesticides" (AMRAP) where a risk assessment scheme for aquatic macrophytes was proposed. Four working groups evolved from this workshop and were charged with the task of developing Tier 1 and higher-tier aquatic macrophyte risk assessment procedures. Subsequently, a SETAC Advisory Group, the Macrophyte Ecotoxicology Group (AMEG) was formed as an umbrella organization for various macrophyte working groups. The purpose of AMEG is to provide scientifically based guidance in all aspects of aquatic macrophyte testing in the laboratory and field, including prospective as well as retrospective risk assessments for chemicals. As AMEG expands, it will begin to address new topics including bioremediation and sustainable management of aquatic macrophytes in the context of ecosystem services.

  11. Hydroponic Uptake of Atrazine and Lambda-cyhalothrin in Aquatic Macrophytes

    Science.gov (United States)

    Bouldin, J. L.; Farris, J. L.; Moore, M. T.; Smith, S.; Cooper, C. M.

    2005-05-01

    Phytoremediation encompasses an array of plant-associated processes known to mitigate contaminants from soil, sediment, and water. Modification of pesticides associated with agricultural runoff includes processes directly associated with aquatic macrophytes in addition to soil geochemical modifications and associated rhizospheric degradation. Remediation attributes of two vegetative species common to agricultural drainages in the Mississippi Delta, USA, were assessed using atrazine and lambda-cyhalothrin. Concentrations used in 8-d hydroponic exposures were calculated using recommended field applications and a 5% runoff model from a 0.65-cm rainfall event on a 2.02-ha field. While greater atrazine uptake was measured in Juncus effusus, greater lambda-cyhalothrin uptake occurred in Ludwigia peploides. Maximum pesticide uptake was reached within 48 h for each exposure and subsequent translocation of pesticides to upper plant biomass occurred in macrophytes exposed to atrazine. Sequestration of 98.2% of lambda-cyhalothrin in roots of L. peploides was measured after 8 d. Translocation of lambda-cyhalothrin in J. effusus resulted in 25.4% of pesticide uptake partitioned to upper plant biomass. These individual macrophyte remediation studies measured species- and pesticide-specific uptake rates, indicating that the seasonality of pesticide applications and macrophyte emergence might interact strongly to enhance mitigation capabilities in edge-of-field conveyance structures.

  12. Diaspore bank of aquatic macrophytes maintaining species diversity in a Neotropical pond

    Directory of Open Access Journals (Sweden)

    Fernando Alves Ferreira

    2016-12-01

    Full Text Available The diaspore bank is an important component of biological diversity acting mainly as storage and source of diaspores, which also indicate dynamics and patterns of maintenance of plant diversity. We tested the hypothesis that the diaspore bank would be the responsible factor for the maintenance of the aquatic plant species, used two techniques to evaluate the diaspore bank: seedling emergence and direct counting of field samples. Density and richness of the diaspore bank were correlated with plant cover of the aquatic macrophyte communities and also with the euphotic zone radiation in different phases of the hydrological cycle. The diaspore bank is a key factor in maintenance of aquatic macrophyte assemblages in this pond. Nymphaea amazonum was one of the dominant species in the pond and presented temporally peaks of plant cover. Furthermore, depth allowed induction of germination of stored diaspores. Therefore, the diaspore bank may predict which species colonizes a site when environmental characteristics are favorable and allows inferring which processes are linked to changes in structure of aquatic macrophyte assemblages. These mechanisms are the means for recovery of macrophyte species under natural disturbances, represented by water level oscillations in floodplains.

  13. [Aquatic macrophytes from a marginal lake of the Embu-mirim river, São Paulo, Brazil].

    Science.gov (United States)

    Beyruth, Z

    1992-08-01

    In the years 1984/1985, limnological surveys were carried out at a marginal pond of the Embu-mirim river belonging to the hydrographic basin of Guarapiranga Reservoir (S. Paulo, Brazil), in order to obtain data for the determination of physical, chemical and biological parameters. Results obtained from the study of the aquatic macrophytes and their relationships with other environmental parameters are analyzed. Eichhornia crassipes (Mart.) Solms, the dominant macrophyte, was observed in several water bodies of that basin, including the river it self, thus favoring an evaluation of its behavior in similar water bodies of the basin.

  14. Quantification of the impact of macrophytes on oxygen dynamics and nitrogen retention in a vegetated lowland river

    NARCIS (Netherlands)

    Desmet, N.J.S.; Van Belleghem, S.; Seuntjens, P.; Bouma, T.J.; Buis, K.; Meire, P.

    2011-01-01

    When macrophytes are growing in the river, the vegetation induces substantial changes to the water quality. Some effects are the result of direct interactions, such as photosynthetic activity or nutrient uptake, whereas others may be attributed to indirect effects of the water plants on

  15. Emergent and floating-leaved macrophytes as refuge for zooplankton in a eutrophic temperate lake without submerged vegetation

    DEFF Research Database (Denmark)

    Cazzanelli, Matteo; Perlt, Trine Warming; Christoffersen, Kirsten Seestern

    2008-01-01

    modifications in the predation pressure, refuge availability and concentration of cyanobacteria in the lake. It is suggested that emergent and floating-leaved macrophytes may play an important role in enhancing water clarity due to increased grazing pressure by zooplankton migrating into the plant stands....... As a consequence, especially in turbid lakes, the ecological role of these functional types of vegetation, and not merely that of submerged macrophyte species, should be taken into consideration.......Several studies have shown that submerged macrophytes provide a refuge for zooplankton against fish predation, whereas the role of emergent and floating-leaved species, which are often dominant in eutrophic turbid lakes, is far less investigated. Zooplankton density in open water and amongst...

  16. AMEG: the new SETAC advisory group on aquatic macrophyte ecotoxicology

    OpenAIRE

    Arts, G.; Davies, J.; Dobbs, M.; Ebke, P.; Hanson, M.; Hommen, U.; Knauer, K.; Loutseti, S.; Maltby, L; Mohr, S; Poovey, A.; Poulsen, V.

    2010-01-01

    \\ud Introduction and background\\ud \\ud Primary producers play critical structural and functional roles in aquatic ecosystems; therefore, it is imperative that the potential risks of toxicants to aquatic plants are adequately assessed in the risk assessment of chemicals. The standard required macrophyte test species is the floating (non-sediment-rooted) duckweed Lemna spp. This macrophyte species might not be representative of all floating, rooted, emergent, and submerged macrophyte species be...

  17. AMEG: the new SETAC advisory group on auqatic macrophyte ecotoxicology

    OpenAIRE

    Arts, G.H.P.; Davies, J.; Dobbs, M.; Ebke, P.; Hanson, M.A.

    2010-01-01

    ntroduction and background Primary producers play critical structural and functional roles in aquatic ecosystems; therefore, it is imperative that the potential risks of toxicants to aquatic plants are adequately assessed in the risk assessment of chemicals. The standard required macrophyte test species is the floating (non-sediment-rooted) duckweed Lemna spp. This macrophyte species might not be representative of all floating, rooted, emergent, and submerged macrophyte species because of dif...

  18. AMEG: the new SETAC advisory group on aquatic macrophyte ecotoxicology

    OpenAIRE

    Arts, G.; Davies, J.; Dobbs, M.; Ebke, P.; Hanson, M; Hommen, U.; Knauer, K; Loutseti, S.; Maltby, L.; Mohr, S.; Poovey, A.; Poulsen, V.

    2010-01-01

    \\ud Introduction and background\\ud \\ud Primary producers play critical structural and functional roles in aquatic ecosystems; therefore, it is imperative that the potential risks of toxicants to aquatic plants are adequately assessed in the risk assessment of chemicals. The standard required macrophyte test species is the floating (non-sediment-rooted) duckweed Lemna spp. This macrophyte species might not be representative of all floating, rooted, emergent, and submerged macrophyte species be...

  19. Remote Sensing supports EULAKES project for mapping submerged macrophytes in Lake Garda

    Science.gov (United States)

    Matta, Erica; Bresciani, Mariano; Giardino, Claudia; Bolpagni, Rossano; Pellegrini, Giovanna; Braga, Federica

    2013-04-01

    Lake bottoms have an important role in the aquatic ecosystem: bathymetry and morphology may affect the hydrodynamic processes in coastal waters, while the presence of aquatic macrophytes helps to preserve the ecology. Within the context of macrophyte monitoring programs, technical advances in remote sensing with higher spatial and spectral resolutions provide opportunities for big scale ecological studies, with the possibility to assess a multitemporal analysis. One of the objectives of the EULAKES project has been to map aquatic vegetation cover inside the Garda Lake from hyperspectral MIVIS (Multispectral Infrared and Visible Imaging Spectrometer) aerial images thanks to the application of a bio-optical model inversion technique (BOMBER: Bio-Optical Model Based tool for Estimating water quality and bottom properties from Remote sensing images). MIVIS images, with a spectral resolution of 102 bands, working between 430 and 1270 nm, were previously corrected for atmospheric, adjacency and glint effects before being processed with the BOMBER tool. One complete MIVIS overflight (12 runs) acquired on June 27th 2011, allowed the retrieval of a macrophyte presence map all around the first 7m deep coastal belt, with a validation error of about 10%, resulting from 89 in situ measures performed during images acquisition. A relevant spatial distribution could be observed, with higher aquatic plants concentration in the Southern part of the lake. A further local cover distribution map localized on the Sirmione Peninsula was integrated with previous results to perform a temporal analysis of macrophyte colonization patterns along this reduced littoral zone for the last 14 years (acquisitions on September 1997, July 2005 and July 2010). Considerable modifications in terms of macrophyte structural complexity and colonized areas were detectable: a drastic reduction of well-established dense communities (more than 70% of cover) and increasing of un-colonized areas were followed by

  20. Dissolved organic carbon release by marine macrophytes

    Directory of Open Access Journals (Sweden)

    C. Barrón

    2012-02-01

    Full Text Available Estimates of dissolved organic carbon (DOC release by marine macrophyte communities (seagrass meadows and macroalgal beds were obtained experimentally using in situ benthic chambers. The effect of light availability on DOC release by macrophyte communities was examined in two communities both by comparing net DOC release under light and dark, and by examining the response of net DOC release to longer-term (days experimental shading of the communities. All most 85% of the seagrass communities and almost all of macroalgal communities examined acted as net sources of DOC. There was a weak tendency for higher DOC fluxes under light than under dark conditions in seagrass meadow. There is no relationship between net DOC fluxes and gross primary production (GPP and net community production (NCP, however, this relationship is positive between net DOC fluxes and community respiration. Net DOC fluxes were not affected by shading of a T. testudinum community in Florida for 5 days, however, shading of a mixed seagrass meadow in the Philippines led to a significant reduction on the net DOC release when shading was maintained for 6 days compared to only 2 days of shading. Based on published and unpublished results we also estimate the global net DOC production by marine macrophytes. The estimated global net DOC flux, and hence export, from marine macrophyte is about 0.197 ± 0.015 Pg C yr−1 or 0.212 ± 0.016 Pg C yr−1 depending if net DOC flux by seagrass meadows was estimated by taking into account the low or high global seagrass area, respectively.

  1. COMPOSTING AQUATIC MACROPHYTES: SALVINIA AURICULATA AND EICHHORNIA

    Directory of Open Access Journals (Sweden)

    Ana Kleiber Pessoa Borges

    2011-07-01

    Full Text Available High population growth and densities in urban areas and the consumerism present in modern societies have pronounced effect on the generation of organic waste, which may become an environmental problem. Aerobic composting is one of the best known alternatives to treating these wastes. This study aimed to evaluate the applicability of composting as an alternative to the disposal of organic wastes from aquatic macrophytes Eichhornia crassipes and Salvinia auriculata collected in the reservoir UHE Luis Eduardo Magalhães, Tocantins, Brazil and also produce an organic compound from different combinations of macrophytes, prunning residues and organic waste generated by the Campus of Palmas of UFT, TO. The study was conducted in an area of 80m² in unprotected environment at the experimental station of the Campus of Palmas. The experiments were done as three replications in the dry season (from 18.09.2008 to 11.21.2008 and rainy season (from 03.09.2009 to 05.04.2009 and the parameters temperature, pH, total nitrogen and carbon, and organic matter were monitored along with counts of microorganisms. It was possible to produce compost from the combinations of organic wastes within 65 days of composting during the dry season and 55 days in the rainy season. The aquatic macrophytes resulted in a good raw material for composting, since there is not a destination for the excess plant materials removed by the cleaning process of the reservoir.

  2. Review of alien marine macrophytes in Tunisia

    Directory of Open Access Journals (Sweden)

    Y. R. SGHAIER

    2014-06-01

    Full Text Available In the present study, the list of alien marine macrophytes introduced into Tunisia was updated in the light of available data and new observations. A total of 27 alien marine macrophytes have been recorded so far from Tunisia: 18 Rhodophyta, 3 Ochrophyta, 5 Chlorophyta and 1 Magnoliophyta. For each species, the locality (-ies, the year (or period and the source of the first observation in Tunisia are given. The distribution and the status (casual, cryptogenic, established or questionable of species in Tunisia were evaluated and, where appropriate, discussed. Among them, Hypnea cornuta is reported for the first time from Tunisia. Fourteen alien marine macrophytes are established, whereas seven cryptogenic and two casual species require further investigation. Eleven species are considered as invasive or potentially invasive in the Mediterranean Sea: Acrothamnion preissii, Asparagopsis armata, A. taxiformis Indo-Pacific lineage, Hypnea cornuta, Lophocladia lallemandii, Womersleyella setacea, Caulerpa chemnitzia, C. cylindracea, C. taxifolia, Codium fragile subsp. fragile and Halophila stipulacea. Finally, the case of four questionable species is also discussed.

  3. Use of low-altitude aerial photography to identify submersed aquatic macrophytes

    Science.gov (United States)

    Schloesser, Donald W.; Manny, Bruce A.; Brown, Charles L.; Jaworski, Eugene

    1987-01-01

    The feasibility of using low-altitude aerial photography to identify beds of submersed macrophytes is demonstrated. True color aerial photos and collateral ground survey information for submersed aquatic macrophyte beds at 10 sites in the St.Clair-Detroit River system were obtained in September 1978. Using the photos and collateral ground survey information, a dichotomous key was developed for the identification of six classes - beds of five genera of macrophytes and one substrate type. A test was prepared to determine how accurately photo interpreters could identify the six classes. The test required an interpreter to examine an unlabeled, outlined area on photographs and identify it using the key. Six interpreters were tested. One pair of interpreters was trained in the interpretation of a variety of aerial photos, a second pair had field experience in the collection and identification of submersed macrophytes in the river system, and a third pair had neither training in the interpretation of aerial photos nor field experience. The criteria that we developed were applied equally well by the interpretors, regardless of their training or experience. Overall accuracy (i.e., omission errors) of all six classes combined was 68% correct, whereas, overall accuracy of individual classes ranged from 50 to 100% correct. Mapping accuracy (i.e. omission and commission errors) of individual classes ranged from 36 to 75%. Although the key developed for this study has only limited application outside the context of the data and sites examined in this study, it is concluded that low-altitude aerial photography, together with limited amounts of collateral ground survey information, can be used to economically identify beds of submersed macrophytes in the St. Clair-Detroit River system and other similar water bodies.

  4. The role of submerged macrophytes and macroalgae in nutrient cycling: A budget approach

    Science.gov (United States)

    Human, Lucienne R. D.; Snow, Gavin C.; Adams, Janine B.; Bate, Guy C.; Yang, Sheng-Chi

    2015-03-01

    This study used a budget approach to determine the effect of submerged macrophytes and macroalgae on the storage of N and P in an estuary. Above and below ground tissue content of nitrogen and phosphorus were determined for the various macrophyte species. The mouth of the estuary was artificially breached in February 2011 with a volume of 0.3 × 106 m3 and closed a week later. A 1:100 year flood with volume close to 3 × 106 m3 breached the mouth naturally in June 2011 flushing water and sediment out of the estuary. In order to track the change in the nutrient acquisition of the submerged macrophytes and macroalgae over a closed-mouth state, the nutrient budget was constructed for the period February 2011 to July 2011, from the time the mouth closed until it opened again. Relative to other inputs the sediment contributed 30% of the TN and 40% TP toward the nutrient budget, while the submerged macrophytes and macroalgae stored 20-30 % TN and 30-38 % TP. The river and precipitation contributed less than 3% of the TN and TP input. It was previously thought that the sediments of South African temporarily open/closed estuaries did not have the necessary organic stock to fuel primary production. However this research showed this to be incorrect. Submerged macrophytes and macroalgae significantly influenced nutrient cycling and this is the first detailed account of incorporating vegetation into a nutrient budget without relying solely on C:N:P ratios.

  5. Predicting Changes in Macrophyte Community Structure from Functional Traits in a Freshwater Lake: A Test of Maximum Entropy Model.

    Science.gov (United States)

    Fu, Hui; Zhong, Jiayou; Yuan, Guixiang; Guo, Chunjing; Lou, Qian; Zhang, Wei; Xu, Jun; Ni, Leyi; Xie, Ping; Cao, Te

    2015-01-01

    Trait-based approaches have been widely applied to investigate how community dynamics respond to environmental gradients. In this study, we applied a series of maximum entropy (maxent) models incorporating functional traits to unravel the processes governing macrophyte community structure along water depth gradient in a freshwater lake. We sampled 42 plots and 1513 individual plants, and measured 16 functional traits and abundance of 17 macrophyte species. Study results showed that maxent model can be highly robust (99.8%) in predicting the species relative abundance of macrophytes with observed community-weighted mean (CWM) traits as the constraints, while relative low (about 30%) with CWM traits fitted from water depth gradient as the constraints. The measured traits showed notably distinct importance in predicting species abundances, with lowest for perennial growth form and highest for leaf dry mass content. For tuber and leaf nitrogen content, there were significant shifts in their effects on species relative abundance from positive in shallow water to negative in deep water. This result suggests that macrophyte species with tuber organ and greater leaf nitrogen content would become more abundant in shallow water, but would become less abundant in deep water. Our study highlights how functional traits distributed across gradients provide a robust path towards predictive community ecology.

  6. The role of pH in structuring communities of Maine wetland macrophytes and chironomid larvae (Diptera)

    Science.gov (United States)

    Woodcock, T.S.; Longcore, J.R.; McAuley, D.G.; Mingo, T.M.; Bennatti, C.R.; Stromborg, K.L.

    2005-01-01

    Aquatic vascular plants, or macrophytes, are an important habitat component for many wetland organisms, and larvae of chironomid midges are ubiquitous components of wetland fauna. Many chironomids are primary consumers of algae and detritus and form an essential energetic link between allochthonous and autochthonous primary production and higher trophic levels, while others are predators and feed on smaller invertebrates. Live macrophytes serve mostly as habitat, whereas plant detritus serves as both habitat and as a food source. Assemblages of macrophytes and chironomid larvae were surveyed in ten Maine wetlands, five with low pH (5.5), and explained in terms of physical and chemical habitat variables. Macrophyte richness was significantly greater, and richness of chironomid larvae was lower, in low pH wetlands. There was no difference in chironomid abundance related to pH. However, community structure was related to pH, suggesting that competitive dominance of a few taxa was responsible for lower richness in low pH wetlands, whereas competition was weaker in high pH wetlands, making coexistence of more chironomid taxa possible. An examination of individual chironomid taxa by stepwise multiple regression showed that distribution of most taxa was controlled by water chemistry variables and macrophyte habit (i.e., floating, submergent).

  7. The ichthyofauna of drifting macrophyte mats in the Ivinhema River, upper Paraná River basin, Brazil

    Directory of Open Access Journals (Sweden)

    Cíntia Karen Bulla

    Full Text Available We describe the fish assemblages associated with drifting macrophyte mats and consider their possible role as dispersal vectors in the Ivinhema River, a major tributary of the upper Paraná River, Brazil. Fish associated with drifting mats were sampled in the main river channel during January and March 2005, when the wind and/or the increased water level were sufficient to transport macrophyte stands. Fish in the drifting mats were sampled with a floating sieve (4 m long x 2 m wide x 0.6 m high, and 2 mm mesh size. In the laboratory, larvae, juvenile, and adult fish were counted and identified to the lowest possible taxonomic level. In four drifting macrophyte mats we captured 218 individuals belonging to at least 28 species, 17 families, and 6 orders. Aphyocharax dentatus, Serrasalmus spp., and Trachelyopterus galeatus were the most abundant taxa associated with the mats, but species richness ranged from 6 to 24 species per mat. In addition, 85% of the total number of individuals caught was larvae and juveniles. Although preliminary and based on limited samples, this study of drifting macrophyte mats was the first one in the last unregulated stretch of the Paraná River remaining inside Brazilian territory, and alerts us to the potential role of macrophytes mats as dispersers of fish species in the region.

  8. The ichthyofauna of drifting macrophyte mats in the Ivinhema River, upper Paraná River basin, Brazil

    Science.gov (United States)

    Bulla, C. K.; Gomes, Luiz Carlos; Miranda, Leandro E.; Agostinho, A. A.

    2011-01-01

    We describe the fish assemblages associated with drifting macrophyte mats and consider their possible role as dispersal vectors in the Ivinhema River, a major tributary of the upper Paraná River, Brazil. Fish associated with drifting mats were sampled in the main river channel during January and March 2005, when the wind and/or the increased water level were sufficient to transport macrophyte stands. Fish in the drifting mats were sampled with a floating sieve (4 m long x 2 m wide x 0.6 m high, and 2 mm mesh size). In the laboratory, larvae, juvenile, and adult fish were counted and identified to the lowest possible taxonomic level. In four drifting macrophyte mats we captured 218 individuals belonging to at least 28 species, 17 families, and 6 orders. Aphyocharax dentatus, Serrasalmus spp., and Trachelyopterus galeatus were the most abundant taxa associated with the mats, but species richness ranged from 6 to 24 species per mat. In addition, 85% of the total number of individuals caught was larvae and juveniles. Although preliminary and based on limited samples, this study of drifting macrophyte mats was the first one in the last unregulated stretch of the Paraná River remaining inside Brazilian territory, and alerts us to the potential role of macrophytes mats as dispersers of fish species in the region.

  9. The influence of submersed macrophytes on the migration of atrazine in water%沉水植物对水体阿特拉津迁移的影响

    Institute of Scientific and Technical Information of China (English)

    瞿梦洁; 李慧冬; 李娜; 张萌; 朱端卫

    2016-01-01

    .50 mg·kg-1. Both of the plants can di-rectly absorb atrazine. When the initial concentration equaled 0.25 mg·kg-1 on the 20th day, the concentration of the atrazine in P. crispus and M. spicatum was equal to 13.4 mg·kg-1 and 11.2 mg·kg-1 respectively. Moreover, both plants have a certain removal effect on atrazine in water. After 45 d, as the concentration increased, the removal rate of the atrazine in the sediments by P. crispus and M. spicatum reached92%, 86%and 91%as well as 84%, 82%and 90%. On the 60 d, the degradation rate of the atrazine in the overlying water reached 35.0%, 51.3%and 1.50%as well as 32.4%, 61.8%and 0.44%. Results show that even though the residue of atrazine in water is easily immobilized by sediments, macrophytes can still be properly applied in a specific concentration range to the removal of atrazine.

  10. Comparison of the community structures of ammonia-oxidizing bacteria and archaea in rhizoplanes of floating aquatic macrophytes.

    Science.gov (United States)

    Wei, Bo; Yu, Xin; Zhang, Shuting; Gu, Li

    2011-09-20

    Some common floating aquatic macrophytes could remove nutrients, such as nitrogen, from eutrophic water. However, the relationship between these macrophytes and the ammonia-oxidizing microorganisms on their rhizoplanes is still unknown. In this study, we examined communities of ammonia-oxidizing archaea (AOA) and bacteria (AOB) on the rhizoplanes of common floating aquatic macrophytes (Eichhornia crassipes, Pistia stratiotes and Ipomoea aquatic) in a eutrophic reservoir.The results show that AOB were the predominant ammonia-oxidizer on the three rhizoplanes. The principal AOB were Nitrosomonas europaea and Nitrosomonas ureae clades. The principal group of AOA was most similar to the clone from activated sludge. The ratio of AOB amoA gene copies to AOA varied from 1.36 (on E. crassipes) to 41.90 (on P. stratiotes). Diversity of AOA was much lower than that of AOB in most samples, with the exception of P. stratiotes.

  11. Study of aquatic macrophytes in the wetlands on the territory of Vrachanski Balkan nature park

    Directory of Open Access Journals (Sweden)

    Valčev Vladimir V.

    2010-01-01

    Full Text Available An overall study of the species composition and abundance of macrophytes in all open water bodies of a large protected territory was made for the first time in Bulgaria. Four high-mountain eutrophic lakes and the upper stretches of three rivers within the boundaries of Vrachanski Balkan Nature Park were investigated. Thirty-nine macrophyte species (higher plants and mosses were identified scattered around, or forming more or less distinct vegetation groups. Three of the lakes are new for the country locations of the species Elatine alsinastrum and Peplis portula. The processes of eutrophication are more advanced in the lakes. The habitats formed around the investigated water bodies have been determined. They are three habitats of European Community interest for Bulgaria. The major threats for the investigated wetlands on the territory of the Park are pointed out.

  12. Macrophytes of the Grlište reservoir (Serbia): Fifteen years after its establishment

    OpenAIRE

    Stanković Ž.; Borišev M.; Simić Slobodanka; Vučković Milijana; Igić Ružica; Vidović Milka; Miljanović B.

    2009-01-01

    A large number of macrophytes, often in dense populations, have developed on the Grlište Reservoir, Serbia over a period of 15 years. Fast development of vegetation is a consequence of anthropogenic impact in lake management. The methodology used in this research covered 100% of the water body, including all areas with or without aquatic plants. The results indicate that plant communities are still in the early phase of development. This leaves space for future development of competitor macro...

  13. CADMIUM AND NICKEL CONTENT IN FROZEN SPINACH FROM SLOVAKIA SALES NETWORK

    Directory of Open Access Journals (Sweden)

    Radovan Stanovič

    2014-02-01

    Full Text Available In the present work, we focused on monitoring the content of hazardous elements in retail packs Spinach (Spinacia oleracea, L.. Among the most hazardous substances that can get into the spinach are nitrates, pesticide residues. The heavy metals as cadmium, mercury, cobalt, nickel, lead and copper. Into productive parts of plants may get land through the atmosphere or contaminated water. Spinach is among reigns supreme wheatgrass. It is a rich source of minerals, vitamins and folic acid. For this article we have chosen to risk elements cadmium and nickel, which we compared with the maximum level defined by the Codex Alimentarius Commission of the Slovak Republic and the values that are set out in Commission Regulation EU No. 420/2011 and no. No 1881/2006 setting maximum levels for contaminants in foods. The results obtained suggest that in either case there was no exceeded of monitored contaminants. The average levels observed risk elements were in the following intervals: Cd 0:03 to 0:10 mg.kg-1, Ni 0697-1218 mg.kg-1. The results will show that in the case of cadmium, we had exceeded the limit value in six samples and nickel was not exceeding the limit value.

  14. Regime shift from phytoplankton to macrophyte dominance in a large river: Top-down versus bottom-up effects

    Energy Technology Data Exchange (ETDEWEB)

    Ibanez, Carles, E-mail: carles.ibanez@irta.cat [IRTA Aquatic Ecosystems, Carretera Poble Nou, Km 5.5, 43540 St. Carles de la Rapita, Catalonia (Spain); Alcaraz, Carles; Caiola, Nuno; Rovira, Albert; Trobajo, Rosa [IRTA Aquatic Ecosystems, Carretera Poble Nou, Km 5.5, 43540 St. Carles de la Rapita, Catalonia (Spain); Alonso, Miguel [United Research Services S.L., Urgell 143, 08036 Barcelona, Catalonia (Spain); Duran, Concha [Confederacion Hidrografica del Ebro, Sagasta 24-26, 50071 Zaragoza, Aragon (Spain); Jimenez, Pere J. [Grup Natura Freixe, Major 56, 43750 Flix, Catalonia (Spain); Munne, Antoni [Agencia Catalana de l' Aigua, Provenca 204-208, 08036 Barcelona, Catalonia (Spain); Prat, Narcis [Departament d' Ecologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona Catalonia (Spain)

    2012-02-01

    The lower Ebro River (Catalonia, Spain) has recently undergone a regime shift from a phytoplankton-dominated to a macrophyte-dominated system. This shift is well known in shallow lakes but apparently it has never been documented in rivers. Two initial hypotheses to explain the collapse of the phytoplankton were considered: a) the diminution of nutrients (bottom-up); b) the filtering effect due to the colonization of the zebra mussel (top-down). Data on water quality, hydrology and biological communities (phytoplankton, macrophytes and zebra mussel) was obtained both from existing data sets and new surveys. Results clearly indicate that the decrease in phosphorus is the main cause of a dramatic decrease in chlorophyll and large increase in water transparency, triggering the subsequent colonization of macrophytes in the river bed. A Generalized Linear Model analysis showed that the decrease in dissolved phosphorus had a relative importance 14 times higher than the increase in zebra mussel density to explain the variation of total chlorophyll. We suggest that the described changes in the lower Ebro River can be considered a novel ecosystem shift. This shift is triggering remarkable changes in the biological communities beyond the decrease of phytoplankton and the proliferation of macrophytes, such as massive colonization of Simulidae (black fly) and other changes in the benthic invertebrate communities that are currently investigated. - Highlights: Black-Right-Pointing-Pointer We show a regime shift in a large river from phytoplankton to macrophyte dominance. Black-Right-Pointing-Pointer Two main hypotheses are considered: nutrient decrease and zebra mussel grazing. Black-Right-Pointing-Pointer Phosphorus depletion is found to be the main cause of the phytoplankton decline. Black-Right-Pointing-Pointer We conclude that oligotrophication triggered the colonization of macrophytes. Black-Right-Pointing-Pointer This new regime shift in a river is similar to that described

  15. Macrophyte growth module for the SWAT model – impact of climate change and management on stream ecology

    DEFF Research Database (Denmark)

    Lu, Shenglan; Trolle, Dennis; Erfurt, Jytte

    originates from the INCA-P model (Wade et al. 2002) with an addition of nitrogen stress. In addition, a benthic sediment layer and interaction of nutrients between sediment layer and water column were implemented. The new modules were validated against macrophyte biomass measurements in several Danish...

  16. Aquatic macrophytes in the large, sub-tropical Itaipu Reservoir, Brazil.

    Science.gov (United States)

    Mormul, Roger Paulo; Ferreira, Fernando Alves; Michelan, Thaisa Sala; Carvalho, Priscilla; Silveira, Marcio José; Thomaz, Sidinei Magela

    2010-12-01

    In the last three decades, rapid assessment surveys have become an important approach for measuring aquatic ecosystem biodiversity. These methods can be used to detect anthropogenic impacts and recognize local or global species extinctions. We present a floristic survey of the aquatic macrophytes along the Brazilian margin of the Itaipu Reservoir conducted in 2008 and compare this with a floristic survey conducted ten years earlier. We used ordination analysis to determine whether assemblage composition differed among reservoir arms. Macrophyte species were sampled in each of the 235 sampling stations using a boat, which was positioned inside three places of each macrophyte stand to record species and search for small plants. We also collected submerged plants using a rake with the boat moving at constant velocity for ten minutes. We assigned individual macrophyte species to life form and identified representative species for each life form. A total of 87 macrophyte taxa were identified. The "emergent" life forms contained the highest number of species, followed by "rooted submerged" life forms. The extensive survey of macrophytes undertaken in September 2008 recorded more species than a survey conducted between 1995 and 1998. This could be due to changes in water physico-chemistry, disturbances due to water drawdown and the long period between surveys, which may have allowed natural colonization by other species. Additionally, differences in the classification systems and taxonomic resolution used in the surveys may account for differences in the number of species recorded. Assemblage composition varied among the arms and was affected by underwater radiation (as measured using a Secchi disk) and fetch. Five non-native species were found. Two of these non-native species (Urochloa subquadripara and Hydrilla verticillata) are of special concern because they have a high frequency of occurrence and occupy large marginal areas of the reservoir. Future surveys should be

  17. Functional properties of spinach (Spinacia oleracea L.) phytochemicals and bioactives.

    Science.gov (United States)

    Roberts, Joseph L; Moreau, Régis

    2016-08-10

    Overwhelming evidence indicates that diets rich in fruits and vegetables are protective against common chronic diseases, such as cancer, obesity and cardiovascular disease. Leafy green vegetables, in particular, are recognized as having substantial health-promoting activities that are attributed to the functional properties of their nutrients and non-essential chemical compounds. Spinach (Spinacia oleracea L.) is widely regarded as a functional food due to its diverse nutritional composition, which includes vitamins and minerals, and to its phytochemicals and bioactives that promote health beyond basic nutrition. Spinach-derived phytochemicals and bioactives are able to (i) scavenge reactive oxygen species and prevent macromolecular oxidative damage, (ii) modulate expression and activity of genes involved in metabolism, proliferation, inflammation, and antioxidant defence, and (iii) curb food intake by inducing secretion of satiety hormones. These biological activities contribute to the anti-cancer, anti-obesity, hypoglycemic, and hypolipidemic properties of spinach. Despite these valuable attributes, spinach consumption remains low in comparison to other leafy green vegetables. This review examines the functional properties of spinach in cell culture, animals and humans with a focus on the molecular mechanisms by which spinach-derived non-essential phytochemicals and bioactives, such as glycolipids and thylakoids, impart their health benefits.

  18. Cadmium and chromium removal kinetics from solution by two aquatic macrophytes.

    Science.gov (United States)

    Suñe, N; Sánchez, G; Caffaratti, S; Maine, M A

    2007-01-01

    The aim of this work was to determine chromium and cadmium bioaccumulation processes of two free-floating macrophytes commonly used in wetlands for water treatment: Salvinia herzogii and Pistia stratiotes. Metal removal from the solution involves two stages: a fast one and a slow one. The fast stage of the Cd uptake is significantly different for each species, while it is not significantly different in Cr uptake. The most important processes of Cd uptake are biological ones in S. herzogii and adsorption, chelation and ionic exchange are in P. stratiotes. The main processes of Cr uptake in both macrophytes are adsorption, chelation and ion exchange. The slow stage is different for each species and metal. Cr precipitation induced by roots occurs in P. stratiotes. Cr uptake through leaves is probably the main cause of the increase of Cr in the aerial parts of S. herzogii.

  19. Removal of nutrients and veterinary antibiotics from swine wastewater by a constructed macrophyte floating bed system.

    Science.gov (United States)

    Xian, Qiming; Hu, Lixia; Chen, Hancheng; Chang, Zhizhou; Zou, Huixian

    2010-12-01

    The potential of three varieties of Italian ryegrass (Lolium multiflorum Lam.), Dryan, Tachimasari and Waseyutaka, to improve the water quality of swine wastewater was evaluated using a constructed macrophyte floating bed system. With respect to reductions in levels of nutrients, chemical oxygen demand (COD), and sulfonamide antimicrobials (SAs, including sulfadiazine, sulfamethazine, and sulfamethoxazole), Dryan performed better than Tachimasari and Waseyutaka. For Dryan, total N was reduced by 84.0%, total P by 90.4%, COD by 83.4% and sulfonamide antimicrobials by 91.8-99.5%. Similar results were observed for Tachimasari and Waseyutaka. The results indicated that the treatment of swine wastewater using the constructed macrophyte floating bed system was effective in the removal of nutrients and veterinary antibiotics.

  20. Aquatic macrophytes from Danube Delta lagoons Musura Bay and Zatonul Mare

    Directory of Open Access Journals (Sweden)

    Sava D.,

    2016-05-01

    Full Text Available The Romanian Danube Delta, a unique, young and continuing to grow region situated in the eastern part of Europe, is the largest continuous marshland and the second largest delta on the continent, and also a favorable place for developing a unique flora and fauna in Europe, with many rare and protected species. The predominance of the aquatic environment, led to the existence of a particular macrophytic flora. In this context, the purpose of this paper is to contribute to the study of aquatic macrophytes. The present study took place over two years, between 2013-2014, and in each year a number of expeditions were made in the two lagoons (Musura Bay and Zătonul Mare in different seasons, in order to observe the diverse flora, because, due to seasonal variation in water quality, there might be a significantly seasonality of the vegetation also.

  1. The distribution of radionuclides between the sediments and macrophytes in the cooling pond of the Ignalina NPP - The Distribution of Radionuclides in Freshwater Hydro Ecosystem's Bottom Sediments and Macrophytes depending on the Ecological Conditions

    Energy Technology Data Exchange (ETDEWEB)

    Marciulioniene, D.; Jefanova, O.; Mazeika, J. [Nature Research Centre, Akademijos str. 2, LT-08412 Vilnius, Lietuva (Lithuania)

    2014-07-01

    The distribution of {sup 137}Cs, {sup 60}Co, {sup 54}Mn in the aquatory of lake Drukshiai (the monitoring stations), the coastal area of this lake, the industrial drainage systems channel of the Ignalina NPP and the cooling water channel of the Ignalina NPP was analyzed on the basis of long-term (1988-2009) investigations of radionuclides specific activity in bottom sediments and macrophytes, also the ability of radionuclides falling into lake Drukshiai from the Ignalina NPP through effluents channels was assessed. It was established that {sup 137}Cs, {sup 60}Co and {sup 54}Mn in the bottom sediments and the macrophytes were distributed quite differently in the monitoring stations of lake Drukshiai and the coastal area as well as in the industrial drainage systems channel of the Ignalina NPP and the cooling water channel of the Ignalina NPP. The different characteristics of the sediments, various ecological conditions, as well as the existing anthropogenic environmental factors and the different in the ecological groups of the plants could have had impact on the distribution of {sup 137}Cs, {sup 60}Co and {sup 54}Mn in the bottom sediments and the aquatic plants in lake Drukshiai and the effluents channels of the Ignalina NPP. The {sup 137}Cs, {sup 60}Co and {sup 54}Mn specific activity's values were significantly higher in macrophytes from the industrial drainage systems channel of Ignalina NPP than in macrophytes from the cooling water channel. Nevertheless the specific activities level of these radionuclides differed only slightly in the macrophytes from the areas which were impacted by the effluents channels of the Ignalina NPP. This can be explained by the fact that the phyto-remediation (as the form of auto-purification) of these effluents from the radionuclides had been present in the industrial drainage systems channel of Ignalina NPP before entering the water into lake Drukshiai. (authors)

  2. Trace element concentrations in freshwater mussels and macrophytes as related to those in their environment

    Directory of Open Access Journals (Sweden)

    Gian Maria BEONE

    2003-02-01

    Full Text Available This study was primarily designed to contribute to the debate "Do accumulator species reflect the element contamination level of their environment?" This research was carried out: 1 to know the distribution of 15 trace elements and calcium in shell and soft tissues of three species of freshwater mussels and macrophytes; 2 to compare the accumulation capacity of each trace element by mussels and by eight species of macrophytes and 3 to test the relationships between the metal concentrations in the mussels and macrophytes and those in water and sediments. The variability of element residues in the mussels is the major limit to accumulator monitoring. The most important causes are: seasonal cycle, physical environment and biological factors such as the size, age and growth rate. This research was designed to eliminate the consequence of variability deriving from the season and the environment. To this end the mussels and macrophytes were collected at the same time from the same habitat: Ranco Bay, Lago Maggiore, Northern Italy. In addition, the element concentrations in more size-classes of the most abundant mussel species (Unio pictorum and Dreissena polymorpha were measured. Trace elements were analyzed by Inductive Coupled Plasma-Mass Spectrometry (ICP-MS. By arranging the data in sequences of decreasing element concentrations in the organisms as well as in water and sediments, we were able to compare the accumulating ability of the tested species and evaluate their capacity to reflect environmental availability. Neither the sequences in the shell nor those in the tissues were similar to the sequence in the water. The differences between the sequences of the mussel tissues and those of the sediments were less striking than those between shells and sediments. Similar results were obtained by macrophytes. In conclusion, the results of this study (which mimics the monitoring practice prove that bioaccumulators cannot be used to evaluate the

  3. Biomass production of the aquatic macrophytes Eichhornia crassipes (water hyacinth and Egeria densa (egeria in organic fish farm effluent treatment system / Produção de biomassa das macrófitas aquáticas Eichhornia crassipes (aguapé e Egeria densa (egeria em sistema de tratamento de efluente de piscicultura orgânica

    Directory of Open Access Journals (Sweden)

    Anderson Coldebella

    2008-08-01

    Full Text Available The objective of this research was to evaluate the production of biomass of the aquatic macrophytes water hyacinth (Eichhornia crassipes and egeria (Egeria densa in three hydraulic detention times in a organic pisciculture effluent treatment system. The system was composed for 18 experimental tanks of 2.00 x 1.00 x 0.65m length, width and depth respectively, coated with polypropylene canvas. An entirely randomized 2 macrophytes x 3 hydraulic detention times (HDT and 3 repetitions. The HDT used was 4, 8, and 12 hours. The biomass production was evaluated at the end of the experiment which was extended at 08/07 to 19/08/2006. The water hyacinth showed the best results of biomass production (P0.05. For egeria the treatment that presented the best production of biomass was reached which TDH of 12 hours, being of 0.10 kg.m-2, followed for the HDT of 8 and 4 hours, not differing between the HDT (P>0.05. One concludes that the water hyacinth produced higher biomass than egeria in all of the HDT evaluated.O presente trabalho teve por o objetivo avaliar a produção de biomassa das macrófitas aquáticas aguapé (Eichhornia crassipes e egeria (Egeria densa em um sistema de tratamento de efluente de piscicultura orgânica, sob 3 condições de tempo de detenção hidráulica. O sistema foi composto por 18 tanques experimentais de 2,00 x 1,00 x 0,65 m de comprimento, largura e profundidade, respectivamente, revestidos com lona de polipropileno. O delineamento foi inteiramente casualizado, com 2 macrófitas, 3 tempos de detenção hidráulica (TDH e 3 repetições. Os TDH utilizados foram de 4, 8 e 12 horas. O sistema foi operado de 08/07 a 19/08/2006. A produção de biomassa foi avaliada ao final do experimento. O aguapé apresentou os melhores resultados de produção de biomassa (P0,05. Para a egeria o tratamento que apresentou a melhor produção de biomassa foi no TDH de 12 horas, sendo de 0,10 kg.m- 2, seguido pelos TDH de 8 e 4 horas, não diferindo

  4. Temporal and spatial variation of limnological variables and biomass of different macrophyte species in a Neotropical reservoir (São Paulo - Brazil

    Directory of Open Access Journals (Sweden)

    Flávia Bottino

    2013-12-01

    Full Text Available AIM: This study reports an investigation of limnological characteristics and aquatic macrophyte occurrence in a neotropical reservoir in order to assess the spatio-temporal variation of water and sediment variables and their influence on plant distribution. METHODS: Macrophytes, water and sediment samples were collected from a Brazilian reservoir in different seasons from four main arms of the reservoir. In total sixteen water-sediment variables were analyzed including N:P ratio and Trophic State Index. The plants were collected using a quadrat sampling procedure and the dry weight per sample was measured. MANOVA was performed to evaluate spatial and temporal variation of environmental variables as well as seasonal biomass differences. To assess the relationship among environmental variables and macrophytes an ordination analysis (using Canonical Correspondence Analysis: CCA was carried out. RESULTS: The spatial and temporal variation of limnological variables generated a heterogeneous system which supports the presence of different species of macrophyte. pH, dissolved oxygen and sediment composition were important predictors of Polygonum lapathifolium occurrence while nutrients were associated with Eichhornia crassipes and Pistia stratiotes. Inorganic substances were related to biomass variation of Eichhornia azurea and Myriophyllum aquaticum. CONCLUSIONS: The spatial variation of the environmental variables has caused heterogeneity in the reservoir and it may support the occurrence of different species of macrophyte. Limnological variables highlighted in CCA are important to predict the species occurrence and their control in the study area.

  5. Cellulase and xylanase activity during the decomposition of three aquatic macrophytes in a tropical oxbow lagoon

    Directory of Open Access Journals (Sweden)

    L Sciessere

    2011-09-01

    Full Text Available Due to the connection between enzymatic activity and degradation of different fractions of organic matter, enzyme assays can be used to estimate degradation rates of particulate and dissolved organic carbon in freshwater systems. The aim of this study was to quantify and model the enzymatic degradation involving the decomposition of macrophytes, describing temporal activity of cellulases (EC 3.2.1.4 and EC 3.2.1.91 and xylanase (EC 3.2.1.8 during in situ decomposition of three aquatic macrophytes (Salvinia sp., Eichhornia azurea and Cyperus giganteus on the surface and water-sediment interface (w-s interface of an oxbow lagoon (Óleo lagoon within a natural Brazilian Savanna Reserve. Overall, the enzymatic degradation of aquatic macrophytes in Óleo lagoon occurred during the whole year and was initiated together with leaching. Xylanase production was ca. 5 times higher than cellulase values due to easy access to this compound by cellulolytic microorganisms. Enzymatic production and detritus mass decay were similar on the surface and w-s interface. Salvinia sp. was the most recalcitrant detritus, with low mass decay and enzymatic activity. E. azurea and C. giganteus decomposition rates and enzymatic production were high and similar. Due to the physicochemical homogeneity observed in the Óleo lagoon, the differences between the decay rates of each species are mostly related with detritus chemical quality.

  6. Assessment of the Potential Biological Activity of Low Molecular Weight Metabolites of Freshwater Macrophytes with QSAR

    Directory of Open Access Journals (Sweden)

    Evgeny A. Kurashov

    2016-01-01

    Full Text Available The paper focuses on the assessment of the spectrum of biological activities (antineoplastic, anti-inflammatory, antifungal, and antibacterial with PASS (Prediction of Activity Spectra for Substances for the major components of three macrophytes widespread in the Holarctic species of freshwater, emergent macrophyte with floating leaves, Nuphar lutea (L. Sm., and two species of submergent macrophyte groups, Ceratophyllum demersum L. and Potamogeton obtusifolius (Mert. et Koch, for the discovery of their ecological and pharmacological potential. The predicted probability of anti-inflammatory or antineoplastic activities above 0.8 was observed for twenty compounds. The same compounds were also characterized by high probability of antifungal and antibacterial activity. Six metabolites, namely, hexanal, pentadecanal, tetradecanoic acid, dibutyl phthalate, hexadecanoic acid, and manool, were a part of the major components of all three studied plants, indicating their high ecological significance and a certain universalism in their use by various species of water plants for the implementation of ecological and biochemical functions. This report underlines the role of identified compounds not only as important components in regulation of biochemical and metabolic pathways and processes in aquatic ecological systems, but also as potential pharmacological agents in the fight against different diseases.

  7. Assessment of the Potential Biological Activity of Low Molecular Weight Metabolites of Freshwater Macrophytes with QSAR.

    Science.gov (United States)

    Kurashov, Evgeny A; Fedorova, Elena V; Krylova, Julia V; Mitrukova, Galina G

    2016-01-01

    The paper focuses on the assessment of the spectrum of biological activities (antineoplastic, anti-inflammatory, antifungal, and antibacterial) with PASS (Prediction of Activity Spectra for Substances) for the major components of three macrophytes widespread in the Holarctic species of freshwater, emergent macrophyte with floating leaves, Nuphar lutea (L.) Sm., and two species of submergent macrophyte groups, Ceratophyllum demersum L. and Potamogeton obtusifolius (Mert. et Koch), for the discovery of their ecological and pharmacological potential. The predicted probability of anti-inflammatory or antineoplastic activities above 0.8 was observed for twenty compounds. The same compounds were also characterized by high probability of antifungal and antibacterial activity. Six metabolites, namely, hexanal, pentadecanal, tetradecanoic acid, dibutyl phthalate, hexadecanoic acid, and manool, were a part of the major components of all three studied plants, indicating their high ecological significance and a certain universalism in their use by various species of water plants for the implementation of ecological and biochemical functions. This report underlines the role of identified compounds not only as important components in regulation of biochemical and metabolic pathways and processes in aquatic ecological systems, but also as potential pharmacological agents in the fight against different diseases.

  8. Cadmium and chromium removal kinetics from solution by two aquatic macrophytes

    Energy Technology Data Exchange (ETDEWEB)

    Sune, N. [Quimica Analitica-Facultad de Ingenieria Quimica, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), U.N.L. Sgo del Estero 2829, 3000 Santa Fe (Argentina); Sanchez, G. [Quimica Analitica-Facultad de Ingenieria Quimica, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), U.N.L. Sgo del Estero 2829, 3000 Santa Fe (Argentina); Caffaratti, S. [Quimica Analitica-Facultad de Ingenieria Quimica, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), U.N.L. Sgo del Estero 2829, 3000 Santa Fe (Argentina); Maine, M.A. [Quimica Analitica-Facultad de Ingenieria Quimica, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), U.N.L. Sgo del Estero 2829, 3000 Santa Fe (Argentina)]. E-mail: amaine@fiqus.unl.edu.ar

    2007-01-15

    The aim of this work was to determine chromium and cadmium bioaccumulation processes of two free-floating macrophytes commonly used in wetlands for water treatment: Salvinia herzogii and Pistia stratiotes. Metal removal from the solution involves two stages: a fast one and a slow one. The fast stage of the Cd uptake is significantly different for each species, while it is not significantly different in Cr uptake. The most important processes of Cd uptake are biological ones in S. herzogii and adsorption, chelation and ionic exchange are in P. stratiotes. The main processes of Cr uptake in both macrophytes are adsorption, chelation and ion exchange. The slow stage is different for each species and metal. Cr precipitation induced by roots occurs in P. stratiotes. Cr uptake through leaves is probably the main cause of the increase of Cr in the aerial parts of S. herzogii. - Cd uptake processes are biological processes in S. herzogii and adsorption, chelation and ionic exchange are in P. stratiotes, whereas Cr uptake processes in both macrophytes are adsorption, chelation and ion exchange.

  9. Assessment of the Potential Biological Activity of Low Molecular Weight Metabolites of Freshwater Macrophytes with QSAR

    Science.gov (United States)

    Fedorova, Elena V.; Krylova, Julia V.

    2016-01-01

    The paper focuses on the assessment of the spectrum of biological activities (antineoplastic, anti-inflammatory, antifungal, and antibacterial) with PASS (Prediction of Activity Spectra for Substances) for the major components of three macrophytes widespread in the Holarctic species of freshwater, emergent macrophyte with floating leaves, Nuphar lutea (L.) Sm., and two species of submergent macrophyte groups, Ceratophyllum demersum L. and Potamogeton obtusifolius (Mert. et Koch), for the discovery of their ecological and pharmacological potential. The predicted probability of anti-inflammatory or antineoplastic activities above 0.8 was observed for twenty compounds. The same compounds were also characterized by high probability of antifungal and antibacterial activity. Six metabolites, namely, hexanal, pentadecanal, tetradecanoic acid, dibutyl phthalate, hexadecanoic acid, and manool, were a part of the major components of all three studied plants, indicating their high ecological significance and a certain universalism in their use by various species of water plants for the implementation of ecological and biochemical functions. This report underlines the role of identified compounds not only as important components in regulation of biochemical and metabolic pathways and processes in aquatic ecological systems, but also as potential pharmacological agents in the fight against different diseases. PMID:27200207

  10. Comparability of river quality assessment using macrophytes: a multi-step procedure to overcome biogeographical differences.

    Science.gov (United States)

    Aguiar, F C; Segurado, P; Urbanič, G; Cambra, J; Chauvin, C; Ciadamidaro, S; Dörflinger, G; Ferreira, J; Germ, M; Manolaki, P; Minciardi, M R; Munné, A; Papastergiadou, E; Ferreira, M T

    2014-04-01

    This paper exposes a new methodological approach to solve the problem of intercalibrating river quality national methods when a common metric is lacking and most of the countries share the same Water Framework Directive (WFD) assessment method. We provide recommendations for similar works in future concerning the assessment of ecological accuracy and highlight the importance of a good common ground to make feasible the scientific work beyond the intercalibration. The approach herein presented was applied to highly seasonal rivers of the Mediterranean Geographical Intercalibration Group for the Biological Quality Element Macrophytes. The Mediterranean Group of river macrophytes involved seven countries and two assessment methods with similar acquisition data and assessment concept: the Macrophyte Biological Index for Rivers (IBMR) for Cyprus, France, Greece, Italy, Portugal and Spain, and the River Macrophyte Index (RMI) for Slovenia. Database included 318 sites of which 78 were considered as benchmarks. The boundary harmonization was performed for common WFD-assessment methods (all countries except Slovenia) using the median of the Good/Moderate and High/Good boundaries of all countries. Then, whenever possible, the Slovenian method, RMI was computed for the entire database. The IBMR was also computed for the Slovenian sites and was regressed against RMI in order to check the relatedness of methods (R(2)=0.45; p<0.00001) and to convert RMI boundaries into the IBMR scale. The boundary bias of RMI was computed using direct comparison of classification and the median boundary values following boundary harmonization. The average absolute class differences after harmonization is 26% and the percentage of classifications differing by half of a quality class is also small (16.4%). This multi-step approach to the intercalibration was endorsed by the WFD Regulatory Committee. © 2013 Elsevier B.V. All rights reserved.

  11. Effect of Nd3+ on Photosynthesis of Spinach

    Institute of Scientific and Technical Information of China (English)

    刘超; 洪法水; 王玲; 郑蕾

    2004-01-01

    The effect of Nd3+ on the photosynthesis and the growth of spinach was studied.The results show that Nd3+ improves the growth of spinach and increases chlorophyll content and photosynthetic rate.UV-Vis spectrum indicates that the Soret band of chl-a in spinach with NdCl3 treatment is blue shifted by 2 nm,and the Q band is red shifted by 1 nm,and the ratio of Soret band intensity and Q band intensity increases.FT-IR spectra show that the peak of porphyrin ring in chl-a of spinach with NdCl3 treatment is widened,suggesting that the formation of Nd3+-chl-a.Treated by NdCl3,the fluorescence emission peak of PSⅡ in spinach leaves is blue shifted by 12 nm and the intensity declines obviously,indicating that Nd3+ is bound to the PSⅡ protein-pigment complex and the electron transfer rate increases.

  12. Alternative soaking media for the FDA procedure in the detection of salmonella from tomatoes and spinach leaf using phage magnetoelastic biosensors

    Science.gov (United States)

    Chen, I.-Hsuan; Hu, Jiajia; Wang, Fengen; Horikawa, Shin; Barbaree, James M.; Chin, Bryan A.

    2016-05-01

    Efforts were made to incorporate the phage Magnetoelastic (ME) biosensor in FDA's Spinach Soaking procedures according to FDA 2015 BAM method. Three soaking materials (Lactose broth, LB broth, and Peptone water) and various soaking times were investigated. Using merely 100 Salmonella cells spiked on the produce surfaces, the phage biosensors detected Salmonella within 5 hours when soaking tomatoes in LB broth as opposed to taking up to 24 hours. Salmonella was detected on spinach leaves within 7 hours. These phage ME biosensors provide a promising rapid detection platform using LB broth in FDA's soaking procedures while shortening the incubation period.

  13. Fast separation and analysis of water-soluble vitamins in spinach by capillary electrophoresis with high voltage%毛细管电泳法高压快速分离分析菠菜中的水溶性维生素

    Institute of Scientific and Technical Information of China (English)

    胡晓琴; 尤慧艳

    2009-01-01

    In capillary electrophoresis, 0 -40 kV (even higher) voltage can be reached by a connecting double-model high voltage power supply. In the article, water-soluble vitamins, VB_1, VB_2, VB_6, VC, calcium D-pantothenate, D-biotin, nicotinic acid and folic acid in vegeta-ble , were separated by using the high voltage power supply under the condition of electrolyte water solution as running buffer. The separation conditions, such as voltage, the concentration of buffer and pH value etc. , were optimized during the experiments. The results showed that eight water-soluble vitamins could be baseline separated in 2. 2 min at 40 kV applied voltage, 25 mmol/L sodium tetraborate buffer solution (pH 8. 8). The water-soluble vitamins in spinach were quantified and the results were satisfied. The linear correlation coefficients of the water-soluble vitamins ranged from 0. 998 1 to 0. 999 9. The detection limits ranged from 0. 2 to 0. 3 mg/L. The average recoveries ranged from 88. 0% to 100. 6% with the relative standard devia-tions (RSD) range of 1. 15% -4. 13% for the spinach samples.%在毛细管电泳法中,通过双模对接高压电源可以获得0~40 kV甚至40 kV以上的超高电压.本研究在40 kV的超高电压下,以纯电解质水溶液为缓冲液,实现了蔬菜中通常含有的8种水溶性维生素(VB_1、VB_2、VB_6、VC、D-泛酸钙、D-生物素、烟酸和叶酸)的快速分离及菠菜样品的定量分析.通过考察电压、缓冲溶液浓度、pH值等因素对分离的影响,确定了优化的实验条件.结果表明,在40 kV高压下,采用25 mmol/L 硼砂-硼酸溶液缓冲液(pH 8.8),菠菜中上述8种水溶性维生素在2.2 min内获得了较好的基线分离.用此方法对菠菜中的水溶性维生素进行定量分析,得到了令人满意的结果.水溶性维生素的线性相关系数范围为 0.998 1~0.999 9,检出限为0.2~0.3 mg/L,在菠菜中的平均加标回收率为88.0% ~100.6%,峰面积的相对标准偏差(RSD)为1

  14. Effect of paclobutrazol on three different aquatic macrophytes under ...

    African Journals Online (AJOL)

    admin

    2013-09-25

    Sep 25, 2013 ... macrophytes under in vitro monoculture or polyculture conditions ... provide many beneficial ecological services, but they could also be problematic .... addition, it was also important to remove all the leaves from the explants.

  15. Oligochaeta (Annelida: Clitellata) associated to aquatic macrophytes in Brazil

    OpenAIRE

    Nathalie Aparecida de Oliveira Sanches; Marina Gulo Alcorinte; Lucas Henrique Sahm; Guilherme Rossi Gorni; Maria Lúcia Ribeiro

    2016-01-01

    Oligochaeta are still characterized as a poorly studied group among the aquatic macroinvertebrates and few studies about their ecology were conducted in Brazil. Thus, our study aimed to provide an overview of the association between Oligochaeta and macrophytes, in Brazilian continental aquatic environments, by means of a literature review along with an inventory of species associated to aquatic macrophytes on marginal lagoons in the reservoir Ribeirão das Anhumas (Américo Brasiliense, São Pau...

  16. Aquatic macrophyte diversity of the Pantanal wetland and upper basin

    OpenAIRE

    VJ. Pott; Pott, A; LCP. Lima; SN. Moreira; AKM Oliveira

    2011-01-01

    This is a short review of the state of the art concerning diversity of aquatic macrophytes and the main aquatic vegetation types in the Brazilian Pantanal wetland and upper watershed. There are ca. 280 species of aquatic macrophytes on the Pantanal floodplain, with scarce endemism. On the upper watershed, Cerrado wetlands (veredas) and limestone springs have a distinct flora from the Pantanal, with twice the species richness. As a representative case of aquatic habitats influenced by river fl...

  17. Characteristics and degradation of carbon and phosphorus from aquatic macrophytes in lakes: insights from solid-state 13C NMR and solution 31P NMR spectroscopy

    Science.gov (United States)

    Water extractable organic matter (WEOM) derived from macrophytes plays an important role in biogeochemical cycling of nutrients, including carbon (C), nitrogen (N) and phosphorus (P) in lakes. However, reports of their composition and degradation in natural waters are scarce. Therefore, compositions...

  18. Oligochaeta (Annelida: Clitellata associated to aquatic macrophytes in Brazil

    Directory of Open Access Journals (Sweden)

    Nathalie Aparecida de Oliveira Sanches

    2016-09-01

    Full Text Available Oligochaeta are still characterized as a poorly studied group among the aquatic macroinvertebrates and few studies about their ecology were conducted in Brazil. Thus, our study aimed to provide an overview of the association between Oligochaeta and macrophytes, in Brazilian continental aquatic environments, by means of a literature review along with an inventory of species associated to aquatic macrophytes on marginal lagoons in the reservoir Ribeirão das Anhumas (Américo Brasiliense, São Paulo, Brazil. In the review, we analyzed 10 articles, where we obtained data on 41 species. We also sampled 5 macrophyte genera, Egeria, Salvinia, Utricularia, Eleocharis, and Ceratophyllum, in August and December 2012 and in March and April 2013, in the reservoir Ribeirão das Anhumas. We registered 21 Oligochaeta species associated to these macrophytes. With the data obtained in the review along with the inventory of the reservoir Ribeirão das Anhumas, we found a total of 41 species associated to aquatic macrophytes, with a higher richness of the Naididae family (93.33%, followed by Opistocystidae (4.44%, and Alluroididae (2.22%. Our study inventoried about 48% of the Oligochaeta diversity registered in continental ecosystems in Brazil, thus highlighting the significance of macrophytes as a resource for these invertebrates, mainly for the Naididae family.

  19. Recognition of prokaryotic transcription terminators by spinach chloroplast RNA polymerase.

    OpenAIRE

    Chen,L.J.; Orozco, E M

    1988-01-01

    To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro, we have fused the spinach chloroplast rbcL promoter to the 3' end of the rbcL gene as well as to various factor independent transcription terminators from E. coli. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RNA polymerase did terminate transcription with varying efficiency at the thra, rrnB, rrnC and gene 32...

  20. Morphometry and retention time as forcing functions to establishment and maintenance of aquatic macrophytes in a tropical reservoir

    Directory of Open Access Journals (Sweden)

    M. B. Cunha-Santino

    limnological variables and macrophytes distribution, in the case of Ourinhos Reservoir, the results pointed out that the water turbulence, low DL and wind exposure are the main driving forces that determine its aquatic plant distribution, life forms and species composition.

  1. Morphometry and retention time as forcing functions to establishment and maintenance of aquatic macrophytes in a tropical reservoir.

    Science.gov (United States)

    Cunha-Santino, M B; Fushita, A T; Peret, A C; Bianchini-Junior, I

    2016-05-03

    variables and macrophytes distribution, in the case of Ourinhos Reservoir, the results pointed out that the water turbulence, low DL and wind exposure are the main driving forces that determine its aquatic plant distribution, life forms and species composition.

  2. How TK-TD and population models for aquatic macrophytes could support the risk assessment for plant protection products.

    Science.gov (United States)

    Hommen, Udo; Schmitt, Walter; Heine, Simon; Brock, Theo Cm; Duquesne, Sabine; Manson, Phil; Meregalli, Giovanna; Ochoa-Acuña, Hugo; van Vliet, Peter; Arts, Gertie

    2016-01-01

    This case study of the Society of Environmental Toxicology and Chemistry (SETAC) workshop MODELINK demonstrates the potential use of mechanistic effects models for macrophytes to extrapolate from effects of a plant protection product observed in laboratory tests to effects resulting from dynamic exposure on macrophyte populations in edge-of-field water bodies. A standard European Union (EU) risk assessment for an example herbicide based on macrophyte laboratory tests indicated risks for several exposure scenarios. Three of these scenarios are further analyzed using effect models for 2 aquatic macrophytes, the free-floating standard test species Lemna sp., and the sediment-rooted submerged additional standard test species Myriophyllum spicatum. Both models include a toxicokinetic (TK) part, describing uptake and elimination of the toxicant, a toxicodynamic (TD) part, describing the internal concentration-response function for growth inhibition, and a description of biomass growth as a function of environmental factors to allow simulating seasonal dynamics. The TK-TD models are calibrated and tested using laboratory tests, whereas the growth models were assumed to be fit for purpose based on comparisons of predictions with typical growth patterns observed in the field. For the risk assessment, biomass dynamics are predicted for the control situation and for several exposure levels. Based on specific protection goals for macrophytes, preliminary example decision criteria are suggested for evaluating the model outputs. The models refined the risk indicated by lower tier testing for 2 exposure scenarios, while confirming the risk associated for the third. Uncertainties related to the experimental and the modeling approaches and their application in the risk assessment are discussed. Based on this case study and the assumption that the models prove suitable for risk assessment once fully evaluated, we recommend that 1) ecological scenarios be developed that are also

  3. Relative importance of P and N in macrophyte and epilithic algae biomass in a wastewater-impacted oligotrophic river.

    Science.gov (United States)

    Taube, Nadine; He, Jianxun; Ryan, M Cathryn; Valeo, Caterina

    2016-08-01

    The role of nutrient loading on biomass growth in wastewater-impacted rivers is important in order to effectively optimize wastewater treatment to avoid excessive biomass growth in the receiving water body. This paper directly relates wastewater treatment plant (WWTP) effluent nutrients (including ammonia (NH3-N), nitrate (NO3-N) and total phosphorus (TP)) to the temporal and spatial distribution of epilithic algae and macrophyte biomass in an oligotrophic river. Annual macrophyte biomass, epilithic algae data and WWTP effluent nutrient data from 1980 to 2012 were statistically analysed. Because discharge can affect aquatic biomass growth, locally weighted scatterplot smoothing (LOWESS) was used to remove the influence of river discharge from the aquatic biomass (macrophytes and algae) data before further analysis was conducted. The results from LOWESS indicated that aquatic biomass did not increase beyond site-specific threshold discharge values in the river. The LOWESS-estimated biomass residuals showed a variable response to different nutrients. Macrophyte biomass residuals showed a decreasing trend concurrent with enhanced nutrient removal at the WWTP and decreased effluent P loading, whereas epilithic algae biomass residuals showed greater response to enhanced N removal. Correlation analysis between effluent nutrient concentrations and the biomass residuals (both epilithic algae and macrophytes) suggested that aquatic biomass is nitrogen limited, especially by NH3-N, at most sampling sites. The response of aquatic biomass residuals to effluent nutrient concentrations did not change with increasing distance to the WWTP but was different for P and N, allowing for additional conclusions about nutrient limitation in specific river reaches. The data further showed that the mixing process between the effluent and the river has an influence on the spatial distribution of biomass growth.

  4. Variation of nutrient and metal concentrations in aquatic macrophytes along the Rio Cachoeira in Bahia (Brazil).

    Science.gov (United States)

    Klump, Andreas; Bauer, Konrad; Franz-Gerstein, Charis; de Menezes, Max

    2002-07-01

    The use of cuprous fungicides in cocoa production in the southern part of the state of Bahia (Brazil) for decades has caused an accumulation of copper in various components of the cocoa plantations, and a contamination of regional freshwater ecosystems is suspected. Urban and industrial sources are supposed to contribute to water pollution and eutrophication of the Rio Cachoeira, the main river in this region. In order to study the metal contamination and nutritional status of this freshwater ecosystem, samples of the aquatic macrophytes Eichhornia crassipes and Pistia stratiotes were collected at seven sites along the river course. The samples were analysed for their copper, aluminium, chromium, nitrogen and phosphorus concentrations. The levels of heavy metals increased in the downstream direction, particularly in the roots of water hyacinth. A dramatic increase of nitrogen and phosphorus concentrations in water as well as in plant tissues was found in samples collected downstream from the city of Itabuna. Metal input and eutrophication were attributed to agricultural, industrial and urban sources in the region. Biomonitoring of the water quality using aquatic macrophytes as accumulative indicator plants is recommended in addition to chemical water analyses.

  5. Contrasting patterns of free-living bacterioplankton diversity in macrophyte-dominated versus phytoplankton blooming regimes in Dianchi Lake, a shallow lake in China

    Science.gov (United States)

    Wang, Yujing; Li, Huabing; Xing, Peng; Wu, Qinglong

    2016-04-01

    Freshwater shallow lakes typically exhibit two alternative stable states under certain nutrient loadings: macrophyte-dominated and phytoplankton-dominated water regimes. An ecosystem regime shift from macrophytes to phytoplankton blooming typically reduces the number of species of invertebrates and fishes and results in the homogenization of communities in freshwater lakes. We investigated how microbial biodiversity has responded to a shift of the ecosystem regime in Dianchi Lake, which was previously fully covered with submerged macrophytes but currently harbors both ecological states. We observed marked divergence in the diversity and community composition of bacterioplankton between the two regimes. Although species richness, estimated as the number of operational taxonomic units and phylogenetic diversity (PD), was higher in the phytoplankton dominated ecosystem after this shift, the dissimilarity of bacterioplankton community across space decreased. This decrease in beta diversity was accompanied by loss of planktonic bacteria unique to the macrophyte-dominated ecosystem. Mantel tests between bacterioplankton community distances and Euclidian distance of environmental parameters indicated that this reduced bacterial community diff erentiation primarily reflected the loss of environmental niches, particularly in the macrophyte regime. The loss of this small-scale heterogeneity in bacterial communities should be considered when assessing long-term biodiversity changes in response to ecosystem regime conversions in freshwater lakes.

  6. Macrophytes shape trophic niche variation among generalist fishes

    Science.gov (United States)

    Vejřík, Lukáš; Šmejkal, Marek; Čech, Martin; Sajdlová, Zuzana; Frouzová, Jaroslava; Kiljunen, Mikko; Peterka, Jiří

    2017-01-01

    Generalist species commonly have a fundamental role in ecosystems as they can integrate spatially distinct habitats and food-web compartments, as well as control the composition, abundance and behavior of organisms at different trophic levels. Generalist populations typically consist of specialized individuals, but the potential for and hence degree of individual niche variation can be largely determined by habitat complexity. We compared individual niche variation within three generalist fishes between two comparable lakes in the Czech Republic differing in macrophyte cover, i.e. macrophyte-rich Milada and macrophyte-poor Most. We tested the hypothesis that large individual niche variation among generalist fishes is facilitated by the presence of macrophytes, which provides niches and predation shelter for fish and their prey items. Based on results from stable nitrogen (δ15N) and carbon (δ13C) isotopic mixing models, perch (Perca fluviatilis L.) and rudd (Scardinius erythrophthalmus (L.)) showed larger individual variation (i.e., variance) in trophic position in Milada as compared to Most, whereas no significant between-lake differences were observed for roach (Rutilus rutilus (L.)). Contrary to our hypothesis, all the three species showed significantly lower individual variation in the relative reliance on littoral food resources in Milada than in Most. Rudd relied significantly more whereas perch and roach relied less on littoral food resources in Milada than in Most, likely due to prevalent herbivory by rudd and prevalent zooplanktivory by perch and roach in the macrophyte-rich Milada as compared to macrophyte-poor Most. Our study demonstrates how the succession of macrophyte vegetation, via its effects on the physical and biological complexity of the littoral zone and on the availability of small prey fish and zooplankton, can strongly influence individual niche variation among generalist fishes with different ontogenetic trajectories, and hence the overall

  7. Efeito da cobertura de macrófitas flutuantes na qualidade da água em um viveiro de piscicultura - DOI: 10.4025/actascibiolsci.v25i1.2086 Effect of floating macrophyte cover on the water quality in fishpond - DOI: 10.4025/actascibiolsci.v25i1.2086

    Directory of Open Access Journals (Sweden)

    Francisco Manuel de Souza Braga

    2003-04-01

    Full Text Available Com o objetivo de avaliar o impacto das macrófitas aquáticas flutuantes na qualidade da água em um viveiro de piscicultura, foi realizado um estudo durante 23 dias consecutivos com coletas de água realizadas em quatro pontos, sendo três dentro do viveiro e um à entrada de água. Foi verificada uma redução drástica do oxigênio dissolvido dentro do viveiro, chegando a 0.87 mg/L. Não foram observadas diferenças significativas (P > 0.05 para o CO2 total, nitrito e amônia em relação à água de entrada (P1 e à água do viveiro (P2, P3 e P2. A clorofila a apresentou uma relação inversa com o fósforo na água. Dentre os compostos nitrogenados, a amônia apresentou as maiores concentrações, com exceção do ponto de entrada (P1, em que o nitrato foi mais elevado, com 513.33 µg/L, apresentando os maiores valores para condutividade. O pH manteve-se ligeiramente ácido no viveiro, influenciando diretamente as concentrações de CO2 livre na água. Os resultados obtidos evidenciaram que a cobertura de macrófita flutuante promoveu um efeito adverso no meio. Se esta cobertura for utilizada de forma controlada, as plantas aquáticas poderão ter um efeito de impacto positivo, devido à capacidade de redução do fósforo total e nitrato na coluna d’água, como observado neste estudoA study was conducted during 23 days in order to evaluate the impact of floating aquatic macrophyte on the water quality of a fishpond. Water samples were collected in four points, three inside the pond and one in water inlet. Drastic reduction of dissolved oxygen was observed in the pond, down to 0.87 mg/L. No significant differences (P > 0.05 were observed for total CO2, nitrite and ammonia with respect to inlet water (P1 and inside the pond (P2, P3 e P4. Chlorophyll a displayed an inverse relationship with phosphorus. Among nitrogen compounds, ammonia presented the highest concentrations except in water inlet where nitrate was higher, 513.33 µg/l, as

  8. Investigation of Darwin’s naturalization hypothesis in invaded macrophyte communities

    Science.gov (United States)

    Although native macrophytes are beneficial in aquatic ecosystems, invasive macrophytes can cause significant ecological and economic harm. Numerous studies have attributed invasiveness to species’ characteristics, whereas others attribute invasion to biotic and abiotic characteristics of the invaded...

  9. Assessment of the Potential Biological Activity of Low Molecular Weight Metabolites of Freshwater Macrophytes with QSAR

    National Research Council Canada - National Science Library

    Kurashov, Evgeny A; Fedorova, Elena V; Krylova, Julia V; Mitrukova, Galina G

    2016-01-01

    ... (Prediction of Activity Spectra for Substances) for the major components of three macrophytes widespread in the Holarctic species of freshwater, emergent macrophyte with floating leaves, Nuphar lutea (L.) Sm...

  10. Isolation and chemical characterization of phosphatidyl glycerol from spinach leaves

    NARCIS (Netherlands)

    Haverkate, E.; Deenen, L.L.M. van

    1965-01-01

    Pure phosphatidyl glycerol was obtained from spinach leaves after repeated chromatography on silica columns. Ascertainment of the configuration of the hydrolysis products formed by the action of phospholipases C (EC 3.1.4.3) and D (EC 3.1.4.4) demonstrated that this phospholipid is identical with 1,

  11. Spinach as a source of carotenoids, folate and antioxidant activity

    NARCIS (Netherlands)

    Castenmiller, J.J.M.

    2000-01-01

    Fruits and vegetables are generally considered important contributors to a healthy diet and an increased intake of fruits and vegetables is related to a decreased risk of cancers, cardiovascular disease, and other diseases. In this thesis two aspects of spinach, a dark-green, leafy vegetable, are ex

  12. A factor from spinach leaves interacting with chlorophylls

    NARCIS (Netherlands)

    Terpstra, Willemke

    1967-01-01

    A factor has been isolated from spinach leaves that interacts with chlorophyll. This interaction is measurable as an increased light sensitivity and fluorescence capacity of the pigment in an aqueous medium. The factor is probably a protein. Interaction was also observed with bacteriochlorophyll an

  13. Thermal inactivation of human norovirus surrogates in spinach and measurement of its uncertainty.

    Science.gov (United States)

    Bozkurt, Hayriye; D'souza, Doris H; Davidson, P Michael

    2014-02-01

    Leafy greens, including spinach, have potential for human norovirus transmission through improper handling and/or contact with contaminated water. Inactivation of norovirus prior to consumption is essential to protect public health. Because of the inability to propagate human noroviruses in vitro, murine norovirus (MNV-1) and feline calicivirus (FCV-F9) have been used as surrogates to model human norovirus behavior under laboratory conditions. The objectives of this study were to determine thermal inactivation kinetics of MNV-1 and FCV-F9 in spinach, compare first-order and Weibull models, and measure the uncertainty associated with the process. D-values were determined for viruses at 50, 56, 60, 65, and 72 °C in 2-ml vials. The D-values calculated from the first-order model (50 to 72 °C) ranged from 0.16 to 14.57 min for MNV-1 and 0.15 to 17.39 min for FCV-9. Using the Weibull model, the tD for MNV-1 and FCV-F9 to destroy 1 log (D ≈ 1) at the same temperatures ranged from 0.22 to 15.26 and 0.27 to 20.71 min, respectively. The z-values determined for MNV-1 were 11.66 ± 0.42 °C using the Weibull model and 10.98 ± 0.58 °C for the first-order model and for FCV-F9 were 10.85 ± 0.67 °C and 9.89 ± 0.79 °C, respectively. There was no difference in D- or z-value using the two models (P > 0.05). Relative uncertainty for dilution factor, personal counting, and test volume were 0.005, 0.0004, and ca. 0.84%, respectively. The major contribution to total uncertainty was from the model selected. Total uncertainties for FCV-F9 for the Weibull and first-order models were 3.53 to 7.56% and 11.99 to 21.01%, respectively, and for MNV-1, 3.10 to 7.01% and 13.14 to 16.94%, respectively. Novel and precise information on thermal inactivation of human norovirus surrogates in spinach was generated, enabling more reliable thermal process calculations to control noroviruses. The results of this study may be useful to the frozen food industry in designing blanching processes for

  14. The influence of aquatic macrophytes on distribution and feeding habit of two Asplanchna species (A. priodonta and A. herrickii in shallow wetlands, South Korea

    Directory of Open Access Journals (Sweden)

    Jong-Yun Choi

    2014-06-01

    Full Text Available We tested the hypothesis that the spatial distribution and diet composition of Asplanchna species might be affected by the presence of aquatic macrophytes in 33 wetlands in South Korea. We estimated the densities of Asplanchna and other rotifer and crustacean, together with environmental parameters, in both vegetated and open water zones, from May to June 2011. In the present study, two species of Asplanchna, A. priodonta and A. herrickii, were observed and significantly more abundant in open water zones lacking macrophytes. In particular, the density of A. priodonta was higher than that of A. herrickii, and the density of A. priodonta was strongly positively correlated with the area of open water. In addition, gut content analysis was used to determine their dietary preferences, with the finding that there was apparent differentiation in food source utilisation between the two Asplanchna species; A. priodonta consumed some protozoa, phytoplankton, and exclusively pelagic rotifer, while A. herrickii consumed primarily Euglena. In particular, Keratella and Polyarthra were most commonly consumed by A. priodonta in open water. Macrophytes represent a suitable habitat for epiphytic rotifer but not for pelagic rotifer; this characteristic drives pelagic rotifer such as Asplanchna towards open water and may be responsible for the significant negative correlation that we observed between macrophyte and Asplanchna densities.

  15. Concurrent removal and accumulation of heavy metals by the three aquatic macrophytes.

    Science.gov (United States)

    Mishra, Virendra Kumar; Tripathi, B D

    2008-10-01

    Under the present investigation effectiveness of three aquatic macrophytes Pistia stratiotes L. (water lettuce), Spirodela polyrrhiza W. Koch (duckweed) and Eichhornia crassipes were tested for the removal of five heavy metals (Fe, Zn, Cu, Cr and Cd). These plants were grown at three different concentrations (1.0, 2.0 and 5.0mgl(-1)) of metals in laboratory experiment. Result revealed high removal (>90%) of different metals during 15 days experiment. Highest removal was observed on 12th day of experiment, thereafter it decreased. Results revealed E. crassipes as the most efficient for the removal of selected heavy metals followed by P. stratiotes and S. polyrrhiza. Results from analysis confirmed the accumulation of different metals within the plant and a corresponding decrease of metals in the water. Significant correlations between metal concentration in final water and macrophytes were obtained. Plants have accumulated heavy metals in its body without the production of any toxicity or reduction in growth. Selected plants shown a wide range of tolerance to all of the selected metals and therefore can be used for large scale removal of heavy metals from waste water.

  16. Polluting macrophytes Colombian lake Fúquene used as substrate by edible fungus Pleurotus ostreatus.

    Science.gov (United States)

    Martínez-Nieto, Patricia; García-Gómez, Gustavo; Mora-Ortiz, Laura; Robles-Camargo, George

    2014-01-01

    Invasive aquatic plants from Lake Fúquene (Cundinamarca, Colombia), water hyacinth (Eichhornia crassipes C. Mart.) and Brazilian elodea (Egeria densa Planch.) have been removed mechanically from the lake and can be used for edible mushrooms production. The growth of the oyster mushroom (Pleurotus ostreatus) on these aquatic macrophytes was investigated in order to evaluate the possible use of fruiting bodies and spent biomass in food production for human and animal nutrition, respectively. Treatments included: water hyacinth, Brazilian elodea, sawdust, rice hulls and their combinations, inoculated with P. ostreatus at 3%. Water hyacinth mixed with sawdust stimulated significantly fruiting bodies production (P = 3.3 × 10(-7)) with 71% biological efficacy, followed by water hyacinth with rice husk (55%) and elodea with rice husk (48%), all of these have protein contents between 26 and 47%. Loss of lignin (0.9-21.6%), cellulose (3.7-58.3%) and hemicellulose (1.9-53.8%) and increment in vitro digestibility (16.7-139.3%) and reducing sugars (73.4-838.4%) were observed in most treatments. Treatments spent biomass presented Relative Forage Values (RFV) from 46.1 to 232.4%. The results demonstrated the fungus degrading ability and its potential use in aquatic macrophytes conversion biomass into digestible ruminant feed as added value to the fruiting bodies production for human nutrition.

  17. Using phytoplankton and macrophytes to assess the trophic and ecological status of some Italian transitional systems

    Science.gov (United States)

    Sfriso, Adriano; Facca, Chiara; Bon, Daniele; Giovannone, Federico; Buosi, Alessandro

    2014-06-01

    Assessment of aquatic ecosystems and their recovery became mandatory with the enactment of the Water Framework Directive (WFD, 2000/60/EC). In this framework, the socio-economic value of transitional waters and their role as natural heritage are particularly important. To improve our knowledge of the ecological status and ecosystem functioning of transitional waters, investigations were carried out in Italian lagoons in the Po Delta and elsewhere (Marano-Grado, Goro, Comacchio, Pialassa Baiona, Lesina and Orbertello), applying the same procedures in accordance with National and International protocols. Nutrient concentrations were measured in water and top sediments and the taxonomic composition of phytoplankton and macrophytes was determined so that the trophic and ecological status could be described also by applying the Macrophyte Quality Index. Although the nutrient load was, on average, similar to that of other lagoons with better status, the overall conditions of Po Delta lagoons were found to be poor or bad, as highlighted by the absence of seagrasses and sensitive macroalgal species. Comparing all considered lagoons, the main factors affecting ecosystem recovery seem to be episodic high nutrient inputs, resulting from changes in river outflows, and huge sediment resuspensions caused by human activities. The studied transitional ecosystems displayed a wide range of conditions from bad to high, highlighting the possibility of ecosystem recovery especially by favouring nutrient regulation and angiosperm proliferation.

  18. Study on the Blanching Process for Quick-frozen Spinach%速冻菠菜漂烫工艺的研究

    Institute of Scientific and Technical Information of China (English)

    李丹

    2011-01-01

    [ Objective ] The aim was to study the blanching process for quick-frozen spinach. [ Method ] The blanching process for quick-frozen spinach was studied, and the blanching conditions were optimized by orthogonal experimental design such as blanching temperature, blanching time, CaCl2 concentration, spinach-water ratio. [ Result ] The best result was spinach-water ratio, blanching temperature and time, CaCl2 concentration were 1∶10, 80 ℃, 20 s, 1.0% respectively. [Conclusion] The study provided reference meaning for time saving, energy consumption saving and reduce cost.%[目的]研究速冻菠菜漂烫最佳工艺条件.[方法]研究漂烫温度、漂烫时间、加水比、氯化钙浓度等漂烫条件对速冻菠菜品质的影响.在此基础上,通过正交试验进一步获得最适漂烫条件.[结果]最佳漂烫工艺:氯化钙浓度1.0%,漂烫温度80℃,料水比1:10,漂烫时间20 s.[结论]该研究对于厂家节约时间、节省能耗、降低成本具有一定借鉴意义.

  19. Water

    Directory of Open Access Journals (Sweden)

    E. Sanmuga Priya

    2017-05-01

    Full Text Available Phytoremediation through aquatic macrophytes treatment system (AMATS for the removal of pollutants and contaminants from various natural sources is a well established environmental protection technique. Water hyacinth (Eichhornia crassipes, a worst invasive aquatic weed has been utilised for various research activities over the last few decades. The biosorption capacity of the water hyacinth in minimising various contaminants present in the industrial wastewater is well studied. The present review quotes the literatures related to the biosorption capacity of the water hyacinth in reducing the concentration of dyestuffs, heavy metals and minimising certain other physiochemical parameters like TSS (total suspended solids, TDS (total dissolved solids, COD (chemical oxygen demand and BOD (biological oxygen demand in textile wastewater. Sorption kinetics through various models, factors influencing the biosorption capacity, and role of physical and chemical modifications in the water hyacinth are also discussed.

  20. Interactions of gold nanoparticles with freshwater aquatic macrophytes are size and species dependent.

    Science.gov (United States)

    Glenn, J Brad; White, Sarah A; Klaine, Stephen J

    2012-01-01

    The partitioning of 4- and 18-nm gold nanoparticles (AuNPs) to aquatic macrophytes was investigated in vivo with exposure suspension in well water. Three morphologically distinct aquatic macrophytes were studied. Myriophyllum simulans Orch. and Egeria densa Planch. are submerged aquatic vascular plants, whereas Azolla caroliniana Willd. is a free-floating aquatic fern. Because aquatic plants absorb the majority of their nutrients from the water column, it is logical to hypothesize that they may absorb nanomaterials in suspension, potentially facilitating trophic transfer. Each plant was exposed to two different-sized gold nanospheres at a nominal concentration of 250 µg/L AuNPs for 24 h. Macrophytes were harvested at six time points (1, 3, 6, 12, 18, and 24 h), dried, and then analyzed for gold concentration via inductively coupled plasma-mass spectrometry. Concentrations were normalized to whole-plant dry tissue mass. The present study shows that absorption of AuNPs through root uptake was size and species dependent. Electron microscopy revealed that 4- and 18-nm AuNPs adsorbed to the roots of each species. Root tissue was sectioned, and transmission electron microscopy indicated that 4-nm and 18-nm AuNPs were absorbed by A. caroliniana, whereas only 4-nm AuNPs were absorbed by M. simulans. Egeria densa did not absorb AuNPs of either size. Gold nanoparticles were confirmed in tissue by using energy-dispersive X-ray spectroscopy. Absorption of AuNPs by plants may be a function of the salinity tolerance of each species.

  1. The effect of foliar feeding of potassium salts and urea in spinach on gas exchange, leaf yield and quality

    Directory of Open Access Journals (Sweden)

    Edward Borowski

    2012-12-01

    Full Text Available In a pot experiment conducted in a phytotron, the effectiveness of foliar feeding of different potassium salts, with and without the addition of 0.5% CO(NH22, in spinach (Spinacia oleracea L. was investigated. Potassium was applied 3 times in the form of 1% solutions KCl, KNO3, K2SO4 and C6H5K3O7•H2O, compared to water as the control treatment. The obtained results show that foliar feeding of potassium salts in spinach is an efficient method of supplementing the level of K+ in plants during vegetation. Plants fed with KNO3 had the highest content of potassium in leaves, and those fertilized with K2SO4, C6H5K3O7 × H2O and KCl had an only slightly lower potassium content. The application of potassium salts resulted in more intensive gas exchange in leaves (stomatal conductance, photosynthesis, transpiration and, as a consequence of that, increased leaf yield. Potassium nitrate and citrate influenced most effectively the abovementioned processes. The treatment of spinach with potassium salts resulted in an increased content of protein, chlorophyll, carotenoids, nitrates and iron as well as a decreased content of vitamin C and calcium in leaves.

  2. The cross-ecosystem impact of deer on an endangered submerged macrophyte, Ranunculus nipponicus var. submersus

    Directory of Open Access Journals (Sweden)

    Hino Takafumi

    2015-07-01

    Full Text Available Macrophytes are a critical component of freshwater ecosystems and are often eaten by cervids. However, the impact of cervids on macrophytes is not well known. In this study, we investigated the effect of sika deer (Cervus nippon yesoensis on the endangered macrophyte Ranunculus nipponicus var. submersus in a spring stream in southwestern Hokkaido, Japan. We monitored the frequency of stream habitat use by deer by using sensor cameras in photography mode for four seasons. We also monitored deer feeding behavior on R. nipponicus var. submersus using sensor cameras in movie mode. To quantitatively evaluate the impact of deer on R. nipponicus var. submersus, we conducted a field experiment in which deer were excluded from part of the stream. We selected 10 pairs of adjacent patches of R. nipponicus var. submersus and set up exclosures covering one patch in each pair. We assessed the frequency of deer feeding and trampling on the control patches using the sensor cameras in photography mode and measured the mean macrophyte stem length in the exclosure and control patches every month for four seasons. To compare abiotic conditions between the exclosure and control patches, we investigated canopy openness, water depth, water temperature, electrical conductivity, pH, current velocity, and water quality at each patch during the growing season. The frequency of deer in the stream habitat was higher from spring to summer than in other seasons. Direct evidence of deer feeding behavior on R. nipponicus var. submersus was recorded using the sensor cameras. Deer often fed on and trampled on the control patches, particularly from spring to summer. The R. nipponicus var. submersus stem length was longer in the exclosure patches than in control patches (P 0.189. Stem growth of R. nipponicus var. submersus differed among seasons (P <0.001, and was low from winter to spring. In addition, exclosure and seasonality significantly affected stem length (P <0.001, and the

  3. 太湖康山湾示范区水生植物对水体氮、磷控制的适用性分析%Applicability analysis of aquatic macrophytes on controlling nitrogen and phosphorus from water in the Kangshan Bay demonstration area of Lake Taihu

    Institute of Scientific and Technical Information of China (English)

    宋玉芝; 朱广伟; 秦伯强

    2013-01-01

    为重建湖泊水生植被,改善太湖局部水域水质,在太湖康山湾示范区两个大型围隔进行了两种类型水生植物重建.通过2010年8月-2011年8月的现场采样及分析测定发现,人工控制条件下,浮叶植物荇菜和菱以及沉水植物马来眼子菜的重建效果较好,在其生长季节具有较高的覆盖度;研究表明,控制风浪及提高透明度是恢复水生植被的前提;植被重建区沉水植物氮、磷含量与浮叶植物差别不大,但浮叶植物重建区水体氮、磷浓度的控制比沉水植物重建区好;从经济及水环境效益角度来看,太湖敞水区的沿岸带由于风浪的控制比较困难,恢复水生植被时,应选择浮叶植物荇菜、菱、沉水植物马来眼子菜等抗风浪能力强的物种.本研究为太湖敞水区沿岸带的生态恢复方案制定提供了理论依据.%In order to restore the aquatic vegetation and improve local water quality, reconstruction of two ecological types of aquatic plants were carried out in two large enclosures in the Kangshan Bay demonstration area of Lake Taihu. By annual investigation, results showed that floating-leaved aquatic plants such as Limnanthemun nymphoides and Trapa bicomi, and submerged plant such as Potamogeton macckianus grew better than other plants under controlled conditions. Their coverage was quite high during the growing season. Results also showed that, controlling wind-driven wave disturbance and improving water transparency were the prerequisite for aquatic vegetation restoration; there was no difference about N and P contents between submerged macrophyte and floating-leaved aquatic plants, but floating-leaved aquatic plants performed better than submerged macrophyte in removing water N and P content. Concerning the economic and environmental benefit, aquatic plants including L. nymphoides , T. bicomi and P. macckianus with powerful anti-wave ability could be selected as the pioneering species to rebuild

  4. Biophysical interactions in fluvial ecosystems: effects of submerged aquatic macrophytes on hydro-morphological processes and ecosystem functioning

    Science.gov (United States)

    Cornacchia, Loreta; Davies, Grieg; Grabowski, Robert; van der Wal, Daphne; van de Koppel, Johan; Wharton, Geraldene; Bouma, Tjeerd

    2016-04-01

    Strong mutual interactions occur at the interface between biota and physical processes in biogeomorphic ecosystems, possibly resulting in self-organized spatial patterns. While these interactions and feedbacks have been increasingly studied in a wide range of landscapes previously, they are still poorly understood in lower energy fluvial systems. Consequently, their impact on the functioning of aquatic ecosystems is largely unknown. In this study we investigate the role of aquatic macrophytes as biological engineers of flow and sediment in lowland streams dominated by water crowfoot (Ranunculus spp.). Using field measurements from two annual growth cycles, we demonstrate that seasonally-changing macrophyte cover maintains relative constant flow rates, both within and between vegetation, despite temporal changes in channel flow discharge. By means of a mathematical model representing the interaction between hydrodynamics and vegetation dynamics, we reveal that scale-dependent feedbacks between plant growth and flow redistribution explain the influence of macrophytes on stabilizing flow rates. Our analysis reveals important implications for ecosystem functions. The creation of fast-flowing channels allows an adequate conveyance of water throughout the annual cycle; yet, patches also have a significant influence on sediment dynamics leading to heterogeneous habitats, thereby facilitating other species. As a last step we investigate the consequences on stream ecosystem functioning, by exploring the relationship between changes in macrophyte cover and the provision of different ecosystem functions (e.g. water conveyance, sediment trapping). Our results highlight that self-organization promotes the combination of multiple ecosystem functions through its effects on hydrological and morphological processes within biogeomorphic ecosystems.

  5. Evaluation of metal contamination and phytoremediation potential of aquatic macrophytes of East Kolkata Wetlands, India

    Science.gov (United States)

    2016-01-01

    Objectives The present study analyzes metal contamination in sediment of the East Kolkata Wetlands, a Ramsar site, which is receiving a huge amount of domestic and industrial wastewater from surrounding areas. The subsequent uptake and accumulation of metals in different macrophytes are also examined in regard to their phytoremediation potential. Methods Metals like cadmium (Cd), copper (Cu), manganese (Mn), and lead (Pb) were estimated in sediment, water and different parts of the macrophytes Colocasia esculenta and Scirpus articulatus. Results The concentration of metals in sediment were, from highest to lowest, Mn (205.0±65.5 mg/kg)>Cu (29.9±10.2 mg/kg)>Pb (22.7±10.3 mg/kg)>Cd (3.7±2.2 mg/kg). The phytoaccumulation tendency of these metals showed similar trends in both native aquatic macrophyte species. The rate of accumulation of metals in roots was higher than in shoots. There were strong positive correlations (p<0.001) between soil organic carbon (OC) percentage and Mn (r =0.771), and sediment OC percentage and Pb (r=0.832). Cation exchange capacity (CEC) also showed a positive correlation (p<0.001) with Cu (r=0.721), Mn (r=0.713), and Pb (r=0.788), while correlations between sediment OC percentage and Cu (r=0.628), sediment OC percentage and Cd (r=0.559), and CEC and Cd (r=0.625) were significant at the p<0.05 level. Conclusions Bioaccumulation factor and translocation factors of these two plants revealed that S. articulatus was comparatively more efficient for phytoremediation, whereas phytostabilization potential was higher in C. esculenta. PMID:27669754

  6. Proceedings of a Seminar on Water Quality Data Interpretation, 8-9 February 1978, Atlanta, Georgia.

    Science.gov (United States)

    1978-01-01

    Macrophyte emergent sediment floating -leaved sediment and water submergent sediment and water free- floating water Algae...the primary producers are the algae and macrophytes ("large plants"). Because of their significant interactions with other components of the aquatic...Example emergent cattail floating -leaved water lily submergent pondweed free- floating duckweed Members of the first three categories ( emergent , floating

  7. Use of aquatic macrophytes in substrate composition to produce moringa seedlings

    Directory of Open Access Journals (Sweden)

    Walda Monteiro Farias

    2016-03-01

    Full Text Available The use of aquatic macrophytes in substrate composition to produce seedlings of moringa is a sustainable alternative. Therefore, the objective of this research was to evaluate the development of moringa seedlings using substrates composed with aquatic macrophytes, and to determine concentrations of N, P and K in the seedlings. We used different combinations of weeds (M, manure (E and topsoil (TV to compose the substrates. The experiment was conducted in a 3 × 4 factorial in randomized arrangement with four replications. We evaluated plant height, crown diameter and stem, relative growth rate in height, canopy diameter and in stem, dry matter of aerial part and of roots, root length and root/shoot ratio, besides the content of N, P and K in seedlings. Moringa seedlings showed reduced growth when produced in substrates composed only with cattail. Water lettuce and substrates composed of 60% M + 30%E + 10 % TV and 70% M + 30% E, promoted greater nutrition and growth of moringa seedlings. The substrate 60M +30E +10TV composed by water hyacinth and cattail resulted in greater amount of P in moringa seedlings.

  8. Cellulase activity and dissolved organic carbon release from lignocellulose macrophyte-derived in four trophic conditions.

    Science.gov (United States)

    Bottino, Flávia; Cunha-Santino, Marcela Bianchessi; Bianchini, Irineu

    2016-01-01

    Considering the importance of lignocellulose macrophyte-derived for the energy flux in aquatic ecosystems and the nutrient concentrations as a function of force which influences the decomposition process, this study aims to relate the enzymatic activity and lignocellulose hydrolysis in different trophic statuses. Water samples and two macrophyte species were collected from the littoral zone of a subtropical Brazilian Reservoir. A lignocellulosic matrix was obtained using aqueous extraction of dried plant material (≈40°C). Incubations for decomposition of the lignocellulosic matrix were prepared using lignocelluloses, inoculums and filtered water simulating different trophic statuses with the same N:P ratio. The particulate organic carbon and dissolved organic carbon (POC and DOC, respectively) were quantified, the cellulase enzymatic activity was measured by releasing reducing sugars and immobilized carbon was analyzed by filtration. During the cellulose degradation indicated by the cellulase activity, the dissolved organic carbon daily rate and enzyme activity increased. It was related to a fast hydrolysable fraction of cellulose that contributed to short-term carbon immobilization (ca. 10 days). After approximately 20 days, the dissolved organic carbon and enzyme activity were inversely correlated suggesting that the respiration of microorganisms was responsible for carbon mineralization. Cellulose was an important resource in low nutrient conditions (oligotrophic). However, the detritus quality played a major role in the lignocelluloses degradation (i.e., enzyme activity) and carbon release.

  9. Cellulase activity and dissolved organic carbon release from lignocellulose macrophyte-derived in four trophic conditions

    Directory of Open Access Journals (Sweden)

    Flávia Bottino

    2016-06-01

    Full Text Available Abstract Considering the importance of lignocellulose macrophyte-derived for the energy flux in aquatic ecosystems and the nutrient concentrations as a function of force which influences the decomposition process, this study aims to relate the enzymatic activity and lignocellulose hydrolysis in different trophic statuses. Water samples and two macrophyte species were collected from the littoral zone of a subtropical Brazilian Reservoir. A lignocellulosic matrix was obtained using aqueous extraction of dried plant material (≈40 °C. Incubations for decomposition of the lignocellulosic matrix were prepared using lignocelluloses, inoculums and filtered water simulating different trophic statuses with the same N:P ratio. The particulate organic carbon and dissolved organic carbon (POC and DOC, respectively were quantified, the cellulase enzymatic activity was measured by releasing reducing sugars and immobilized carbon was analyzed by filtration. During the cellulose degradation indicated by the cellulase activity, the dissolved organic carbon daily rate and enzyme activity increased. It was related to a fast hydrolysable fraction of cellulose that contributed to short-term carbon immobilization (ca. 10 days. After approximately 20 days, the dissolved organic carbon and enzyme activity were inversely correlated suggesting that the respiration of microorganisms was responsible for carbon mineralization. Cellulose was an important resource in low nutrient conditions (oligotrophic. However, the detritus quality played a major role in the lignocelluloses degradation (i.e., enzyme activity and carbon release.

  10. Mowing Submerged Macrophytes in Shallow Lakes with Alternative Stable States: Battling the Good Guys?

    Science.gov (United States)

    Kuiper, Jan J.; Verhofstad, Michiel J. J. M.; Louwers, Evelien L. M.; Bakker, Elisabeth S.; Brederveld, Robert J.; van Gerven, Luuk P. A.; Janssen, Annette B. G.; de Klein, Jeroen J. M.; Mooij, Wolf M.

    2017-04-01

    Submerged macrophytes play an important role in maintaining good water quality in shallow lakes. Yet extensive stands easily interfere with various services provided by these lakes, and harvesting is increasingly applied as a management measure. Because shallow lakes may possess alternative stable states over a wide range of environmental conditions, designing a successful mowing strategy is challenging, given the important role of macrophytes in stabilizing the clear water state. In this study, the integrated ecosystem model PCLake is used to explore the consequences of mowing, in terms of reducing nuisance and ecosystem stability, for a wide range of external nutrient loadings, mowing intensities and timings. Elodea is used as a model species. Additionally, we use PCLake to estimate how much phosphorus is removed with the harvested biomass, and evaluate the long-term effect of harvesting. Our model indicates that mowing can temporarily reduce nuisance caused by submerged plants in the first weeks after cutting, particularly when external nutrient loading is fairly low. The risk of instigating a regime shift can be tempered by mowing halfway the growing season when the resilience of the system is highest, as our model showed. Up to half of the phosphorus entering the system can potentially be removed along with the harvested biomass. As a result, prolonged mowing can prevent an oligo—to mesotrophic lake from becoming eutrophic to a certain extent, as our model shows that the critical nutrient loading, where the lake shifts to the turbid phytoplankton-dominated state, can be slightly increased.

  11. Oxidative stress responses of submerged macrophyte Vallisneria asiatica to different concentrations of cyanobacteria

    Science.gov (United States)

    Kang, Caixia; Kuba, Takahiro; Hao, Aimin; Iseri, Yasushi; Li, Chunjie; Zhang, Zhenjia

    2015-03-01

    In a 10-day aquarium experiment, this investigation examines macrophyte restoration in eutrophic Lake Taihu, the physiological effects of different plant biomass levels and of increasing natural cyanobacterial concentrations on a submerged macrophyte, Vallisneria asiatica. Cyanobacterial stress suppressed the superoxide dismutase (SOD) activity of the plant's leaves and induced the catalase (CAT) and peroxidase (POD) activities of its roots. The soluble protein content in V. asiatica decreased with an increase in natural cyanobacterial concentrations, whereas the malonaldehyde (MDA) increased significantly at chlorophyll a (Chl a) concentrations of 222 and 262 μg/L in water. V. asiatica adapted to the stress caused by cyanobacterial concentrations by adjusting its antioxidant defense system to remove the excessive reactive oxygen species when the algal Chl a concentration was >109 μg/L. Additionally, high biomass of V. asiatica (2 222 g FW/m2) can inhibit the reproduction of cyanobacteria more significantly than low biomass (1 111 g FW/m2). High biomass of V. asiatica increased the oxidative stress in an individual plant when the initial Chl a concentration in the water reached 222 and 262 μg/L, as expressed by the increased MDA in leaves, compared with low biomass of V. asiatica. This provides a basis for controlling cyanobacterial concentrations and V. asiatica biomass for the recovery of V. asiatica in eutrophic Lake Taihu.

  12. Popeye strikes again: The deep proteome of spinach leaves.

    Science.gov (United States)

    Fasoli, Elisa; D'Amato, Alfonsina; Kravchuk, Alexander V; Boschetti, Egisto; Bachi, Angela; Righetti, Pier Giorgio

    2011-01-01

    The cytoplasmic proteome of spinach leaves (Spinacia oleracea L) has been investigated with the help of commercially available (ProteoMiner) combinatorial peptide ligand libraries and with home-made ligand beads as prepared in our laboratory. The protein capture had been performed at three pH values (4.0, 7.0 and 9.3) and elution performed in 4% boiling SDS, 20mM DTT. The total number of unique gene products identified amounts to 322 proteins, of which 114 are in common with the control, untreated sample, 18 are present only in the control and 190 represent the new species detected with the help of all combined eluates and likely represent low-abundance species. This is the first in depth exploration of the spinach cytoplasmic proteome and might enable further studies on interaction, regulation and expression of proteins biological processes in combination or not with transcriptomics data.

  13. Live-cell imaging of mammalian RNAs with Spinach2

    Science.gov (United States)

    Strack, Rita L.; Jaffrey, Samie R.

    2015-01-01

    The ability to monitor RNAs of interest in living cells is crucial to understanding the function, dynamics, and regulation of this important class of molecules. In recent years, numerous strategies have been developed with the goal of imaging individual RNAs of interest in living cells, each with their own advantages and limitations. This chapter provides an overview of current methods of live-cell RNA imaging, including a detailed discussion of genetically encoded strategies for labeling RNAs in mammalian cells. This chapter then focuses on the development and use of “RNA mimics of GFP” or Spinach technology for tagging mammalian RNAs, and includes a detailed protocol for imaging 5S and CGG60 RNA with the recently described Spinach2 tag. PMID:25605384

  14. Phenol toxicity to the aquatic macrophyte Lemna paucicostata

    Energy Technology Data Exchange (ETDEWEB)

    Park, Ji-Sook [Division of Life Science, University of Incheon, Incheon 406-840 (Korea, Republic of); Brown, Murray T. [School of Marine Science and Engineering, University of Plymouth, Plymouth, Devon PL4 8AA (United Kingdom); Han, Taejun, E-mail: hanalgae@hanmail.net [Division of Life Science, University of Incheon, Incheon 406-840 (Korea, Republic of); Institute of Green Environmental Research, University of Incheon, Incheon 406-840 (Korea, Republic of)

    2012-01-15

    Phenol is a ubiquitous environmental pollutant and a widely used reference toxicant for many bioassays. However, little information is available regarding the toxic effects of phenol on aquatic macrophytes. Seventy-two hour bioassays, with different end-points, were carried out to assess phenol toxicity in Lemna paucicostata. A concentration-dependent decline in frond multiplication and colony disintegration was observed, with 11.38 and 22.76 {mu}M phenol resulting in browning of fronds and colony disintegration, respectively. Growth of fronds, as measured by changes in surface area, was significantly inhibited with EC{sub 50} value of 2.70 {mu}M. When pulse amplitude modulated chlorophyll a (Chl a) fluorescence imaging (i-PAM) was employed, the maximum quantum yield of PS II (F{sub v}/F{sub m}) significantly declined with increasing phenol concentrations with resultant EC{sub 50} of 1.91 {mu}M and coefficients of variation (CVs) generated for the EC{sub 50} values of less than 4.7%. A gradual increase in fluorescence emissions from chlorophylls a and b and pheophytin up to a concentration of 2.85 {mu}M was found but declined markedly at higher concentrations. The significant correlation between the F{sub v}/F{sub m} and surface growth rate data implies that the former is an appropriate biomarker of whole plant toxicity. Using imaging Chl a fluorescence on L. paucicostata provides a rapid, sensitive and reliable method for assessing the toxic risks posed by phenol to aquatic ecosystems and has practical applications for municipal and industrial waste water management.

  15. Spinach thioredoxin m inhibits DNA synthesis in fertilized Xenopus eggs.

    OpenAIRE

    Hartman, H; Wu, M.; Buchanan, B.B.; Gerhart, J C

    1993-01-01

    A role for thioredoxin in metazoan DNA synthesis has been assessed by injecting rapidly dividing Xenopus eggs with purified heterologous thioredoxins, which might act as inhibitors if they were to replace resident thioredoxins in some but not all reaction steps. Of 10 tested proteins, spinach chloroplast thioredoxin m is the most potent inhibitor. Eggs cleave and produce cells lacking nuclei. DNA synthesis is severely reduced. Development arrests before gastrulation. In egg extracts, thioredo...

  16. Physical, chemical and microbiological aspects during the dry and rainy seasons in a pond covered by macrophyte used in aquaculture water supply Aspectos físico-químicos e microbiológicos nas estações de seca e chuva em viveiro coberto por macrófitas usado no abastecimento de água para aquicultura

    Directory of Open Access Journals (Sweden)

    Samuel Generoso Dias

    2012-09-01

    Full Text Available AIM: The water quality of a pond covered by macrophytes and used as a water supply for aquaculture was evaluated during the dry and rainy seasons; METHODS: Six points were established for water sampling, at water inflow and outflow. Samplings were carried out monthly between June 2008 and May 2009; RESULTS: Inflow points P1, P2 and P3 in the pond had higher nutrient concentrations and high trophic rates. Moreover, capybaras in the area caused sediment suspension and an increase in fecal coliforms. There was significant difference (p OBJETIVO: O objetivo deste estudo foi avaliar durante as estações seca e estação chuvosa a qualidade da água de um viveiro, coberto por macrófitas, utilizado como sistema de abastecimento de água para aquicultura; MÉTODOS: Foram estabelecidos 6 pontos para a coleta das amostras de água na superfície do viveiro, envolvendo entradas e saídas da água. As coletas foram realizadas mensalmente entre junho de 2008 e maio de 2009; RESULTADOS: Os pontos P1, P2 e P3 devido à entrada de água no viveiro apresentaram maiores concentrações de nutrientes com elevado estado trófico e, a entrada de capivaras nestes locais promoveu a suspensão do sedimento e aumento de coliformes fecais. Há significativas diferenças (p < 0,05 nas concentrações de nutrientes na água entre seca e chuva, com o escoamento de água gerado pela precipitação carreando material do entorno do viveiro para a água; CONCLUSÕES: O sistema estudado sofre influência direta da precipitação e da ausência de manejo adequado da área do entorno. Durante a estação chuvosa a qualidade da água ficou comprometida com aumento da concentração de nutrientes, coliformes fecais e redução do oxigênio dissolvido na água, devido ao material alóctone proveniente da área ao redor do viveiro, afetando negativamente o sistema de abastecimento.

  17. Physical, chemical and microbiological aspects during the dry and rainy seasons in a pond covered by macrophyte used in aquaculture water supply Aspectos físico-químicos e microbiológicos nas estações de seca e chuva em viveiro coberto por macrófitas usado no abastecimento de água para aquicultura

    Directory of Open Access Journals (Sweden)

    Samuel Generoso Dias

    2012-01-01

    Full Text Available AIM: The water quality of a pond covered by macrophytes and used as a water supply for aquaculture was evaluated during the dry and rainy seasons; METHODS: Six points were established for water sampling, at water inflow and outflow. Samplings were carried out monthly between June 2008 and May 2009; RESULTS: Inflow points P1, P2 and P3 in the pond had higher nutrient concentrations and high trophic rates. Moreover, capybaras in the area caused sediment suspension and an increase in fecal coliforms. There was significant difference (p OBJETIVO: O objetivo deste estudo foi avaliar durante as estações seca e estação chuvosa a qualidade da água de um viveiro, coberto por macrófitas, utilizado como sistema de abastecimento de água para aquicultura; MÉTODOS: Foram estabelecidos 6 pontos para a coleta das amostras de água na superfície do viveiro, envolvendo entradas e saídas da água. As coletas foram realizadas mensalmente entre junho de 2008 e maio de 2009; RESULTADOS: Os pontos P1, P2 e P3 devido à entrada de água no viveiro apresentaram maiores concentrações de nutrientes com elevado estado trófico e, a entrada de capivaras nestes locais promoveu a suspensão do sedimento e aumento de coliformes fecais. Há significativas diferenças (p < 0,05 nas concentrações de nutrientes na água entre seca e chuva, com o escoamento de água gerado pela precipitação carreando material do entorno do viveiro para a água; CONCLUSÕES: O sistema estudado sofre influência direta da precipitação e da ausência de manejo adequado da área do entorno. Durante a estação chuvosa a qualidade da água ficou comprometida com aumento da concentração de nutrientes, coliformes fecais e redução do oxigênio dissolvido na água, devido ao material alóctone proveniente da área ao redor do viveiro, afetando negativamente o sistema de abastecimento.

  18. Mechanism of lanthanum effect on chlorophyll of spinach

    Institute of Scientific and Technical Information of China (English)

    HONG; Fashui(洪法水); WEI; Zhenggui(魏正贵); ZHAO; Guiwen(赵贵文)

    2002-01-01

    The mechanism of La3+ effect on chlorophyll (chl) of spinach in solution culture has been studied. The results show that La3+ can obviously promote growth, increase chlorophyll contents and photosynthetic rate of spinach. La3+ may substitute Mg2+ for chlorophyll formation of spinach when there is no Mg2+ in solution. La3+ improves significantly PSII formation and enhances electron transport rate of PSII. By ICP-MS and atom absorption spectroscopy methods, it has been revealed that rare earth elements (REEs) can enter chloroplasts and increase Mg2+-chl contents; and REEs bind to chlorophyll and also form REE-chl. REE-chl is about 72% in total chlorophyll with La3+ treatment and without Mg2+ in solution. By UV-Vis, FT-IR and extended X-ray absorption fine structure spectroscopy (EXAFS) methods, it has been found that La3+ coordinates with nitrogen of porphyrin rings with the average La-N bond length of 0.253 nm.

  19. Enzyme-assisted extraction of stabilized chlorophyll from spinach.

    Science.gov (United States)

    Özkan, Gülay; Ersus Bilek, Seda

    2015-06-01

    Zinc complex formation with chlorophyll derivatives in spinach pulp was studied by adding 300ppm Zn(2+) for production of stable food colorant, followed by the heating at 110°C for 15min. Zinc complex formation increased at pH values of 7.0 or greater. Pectinex Ultra SP-L was selected for enzyme-assisted release of zinc-chlorophyll derivatives from spinach pulp. Effect of enzyme concentration (1-9%), treatment temperature (30-60°C), and time (30-210min) on total chlorophyll content (TCC) were optimized using response surface methodology. A quadratic regression model (R(2)=0.9486) was obtained from the experimental design. Optimum treatment conditions were 8% enzyme concentration, 45°C, and 30min, which yielded a 50.747mgTCC/100g spinach pulp. Enzymatic treatment was followed by solvent extraction with ethanol at a solvent-to-sample ratio of 2.5:1 at 60°C for 45min for the highest TCC recovery. Pretreatment with enzyme and extraction in ethanol resulted in 39% increase in Zn-chlorophyll derivative yield.

  20. Enantioselective degradation of metalaxyl in cucumber, cabbage, spinach and pakchoi.

    Science.gov (United States)

    Wang, Meiyun; Zhang, Qing; Cong, Lujing; Yin, Wei; Wang, Minghua

    2014-01-01

    The enantioselective degradation behaviors of the acylamino acid fungicide metalaxyl in vegetables (cucumber, cabbage, spinach and pakchoi) under field and greenhouse conditions were elucidated by reversed-phase high-performance liquid chromatography (HPLC). A cellulose tris (3-chloro-4-methyl phenyl carbamate) chiral column (Lux Cellulose-2) was used for the chiral separation of the enantiomers using acetonitrile-0.1% formic acid solution (40:60, v/v) as mobile phase at a flow rate of 1.0 mL min(-1). The two enantiomers could be completely separated with a resolution (Rs) of 5.2. The linearity range, matrix effect, precision and recovery were evaluated. The method was successfully applied for the enantioselective analysis of metalaxyl in cucumber, cabbage, spinach and pakchoi under different conditions. The results showed that R-enantiomer dissipated faster than S-enantiomer in cucumber of greenhouse. Inversely, a preferential degradation of S-form was found in spinach and pakchoi under field and greenhouse conditions. No stereoselective degradation in cabbage was observed. These results can be applied for evaluating the environmental risk and food safety. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. Distinguishing Bovine Fecal Matter on Spinach Leaves Using Field Spectroscopy

    Directory of Open Access Journals (Sweden)

    Colm D. Everard

    2016-08-01

    Full Text Available Detection of fecal contaminants on leafy greens in the field will allow for decreasing cross-contamination of produce during and post-harvest. Fecal contamination of leafy greens has been associated with Escherichia coli (E. coli O157:H7 outbreaks and foodborne illnesses. In this study, passive field spectroscopy measuring reflectance and fluorescence created by the sun’s light, coupled with numerical normalization techniques, are used to distinguish fecal contaminants on spinach leaves from soil on spinach leaves and uncontaminated spinach leaf portions. A Savitzky-Golay first derivative transformation and a waveband ratio of 710:688 nm as normalizing techniques were assessed. A soft independent modelling of class analogies (SIMCA procedure with a 216 sample training set successfully predicted all 54 test set sample types using the spectral region of 600–800 nm. The ratio of 710:688 nm along with set thresholds separated all 270 samples by type. Application of these techniques in-field to avoid harvesting of fecal contaminated leafy greens may lead to a reduction in foodborne illnesses as well as reduced produce waste.

  2. Chemical warfare in freshwater. Allelpathic effects of macrophytes on phytoplankton

    NARCIS (Netherlands)

    Mulderij, G.

    2006-01-01

    Aquatic macrophytes can excrete chemical substances into their enviroment and these compounds may inhibit the growth of phytoplankton. This process is defined as allelopathy: one organism has effects on another via the excretion of a (mixture of) chemical substance(s). With laboratory and field expe

  3. AMEG: the new SETAC advisory group on auqatic macrophyte ecotoxicology

    NARCIS (Netherlands)

    Arts, G.H.P.; Davies, J.; Dobbs, M.; Ebke, P.; Hanson, M.A.

    2010-01-01

    ntroduction and background Primary producers play critical structural and functional roles in aquatic ecosystems; therefore, it is imperative that the potential risks of toxicants to aquatic plants are adequately assessed in the risk assessment of chemicals. The standard required macrophyte test

  4. AMEG: the new SETAC advisory group on auqatic macrophyte ecotoxicology

    NARCIS (Netherlands)

    Arts, G.H.P.; Davies, J.; Dobbs, M.; Ebke, P.; Hanson, M.A.

    2010-01-01

    ntroduction and background Primary producers play critical structural and functional roles in aquatic ecosystems; therefore, it is imperative that the potential risks of toxicants to aquatic plants are adequately assessed in the risk assessment of chemicals. The standard required macrophyte test spe

  5. Experiments on growth interactions between two invasive macrophyte species

    NARCIS (Netherlands)

    Barrat-Segretain, M-H.; Elger, A.F.

    2004-01-01

    The success of invasive species has been attributed to the ability to displace other species by direct competition. We studied growth and possible competition between the two macrophyte species Elodea nuttallii and E. canadensis, because the former has been observed to replace the latter in the

  6. African Journal of Science and Technology (AJST) MACROPHYTES ...

    African Journals Online (AJOL)

    opiyo

    MACROPHYTES ET GROUPEMENTS VÉGÉTAUX AQUATIQUES. ET AMPHIBIES DE LA ... plaine d'inondation indique que le marnage demeure important et la salinité des terres reste encore élevée. ..... The Journal of Ecology, vol. 54, n° 3 ...

  7. Herbivory on freshwater and marine macrophytes : A review and perspective

    NARCIS (Netherlands)

    Bakker, Elisabeth S.; Wood, Kevin A.; Pages, Jordi F.; Veen, G. F. (Ciska); Christianen, Marjolijn J. A.; Santamaria, Luis; Nolet, Bart A.; Hilt, Sabine

    2016-01-01

    Until the 1990s, herbivory on aquatic vascular plants was considered to be of minor importance, and the predominant view was that freshwater and marine macrophytes did not take part in the food web: their primary fate was the detritivorous pathway. In the last 25 years, a substantial body of

  8. Herbivory on freshwater and marine macrophytes : A review and perspective

    NARCIS (Netherlands)

    Bakker, Elisabeth S.; Wood, Kevin A.; Pages, Jordi F.; Veen, G. F. (Ciska); Christianen, Marjolijn J. A.; Santamaria, Luis; Nolet, Bart A.; Hilt, Sabine

    2016-01-01

    Until the 1990s, herbivory on aquatic vascular plants was considered to be of minor importance, and the predominant view was that freshwater and marine macrophytes did not take part in the food web: their primary fate was the detritivorous pathway. In the last 25 years, a substantial body of evidenc

  9. Chemical warfare in freshwater. Allelpathic effects of macrophytes on phytoplankton

    NARCIS (Netherlands)

    Mulderij, G.

    2006-01-01

    Aquatic macrophytes can excrete chemical substances into their enviroment and these compounds may inhibit the growth of phytoplankton. This process is defined as allelopathy: one organism has effects on another via the excretion of a (mixture of) chemical substance(s). With laboratory and field

  10. Macroinvertebrate communities associated with macrophyte habitats in a tropical man-made lake (Lake Taabo, Côte d’Ivoire

    Directory of Open Access Journals (Sweden)

    Kouamé M. K.

    2011-02-01

    Full Text Available An ecological study was done on Lake Taabo with the main objective of characterising macroinvertebrate communities associated with the microhabitats created mainly by Eichhornia crassipes and other littoral native macrophytes. We sampled organisms in patches of those aquatic macrophytes. Also, some abiotic variables (temperature, transparency, turbidity, pH, TDS, conductivity, dissolved oxygen, NH$_{4}^{+}$+4, NO$_{3}^{-}$−3, NO$_{2}^{-}$−2, PO$_{4}^{3-}$3−4 and SiO$_{2}^{-}$−2 were measured. Overall, forty-three taxa of macroinvertebrates were identified. Ten of them were exclusively associated with water hyacinth while five were only associated with littoral macrophytes. Macroinvertebrate taxa with some of the highest family richness were Gastropoda, Coleoptera, Heteroptera, Odonata and Diptera. The taxon with highest density in both microhabitats was Chironomidae. Although higher values of taxonomic richness (Rs, the Shannon index (H′ and evenness (J were obtained with the water hyacinth habitat, significant differences between the two microhabitats were not observed. Canonical Correspondence Analysis revealed that samples of E. crassipes collected in the dry season were characterised by Gastropoda and Odonata, as well as higher values of transparency and ammonia-nitrogen. Baetidae, Hydrophilidae, Chironomidae, Ceratopogonidae, Coenagrionidae, Naucoridae and Ostracoda were most abundant in both E. crassipes and littoral macrophyte habitats during the rainy season. This season was characterised by higher levels of nitrates and conductivity.

  11. Use of macrophyte plants, sand & gravel materials in constructed wetlands for greywater treatment

    Science.gov (United States)

    Qomariyah, S.; Ramelan, AH; Sobriyah; Setyono, P.

    2017-02-01

    Greywater discharged without any treatments into drainage channels or natural water bodies will lead to environmental degradation and health risk. Local macrophyte plants combined with natural materials of sand and gravel have been used in a system of constructed wetland for the treatment of the greywater. This paper presents the results of some studies of the system carried out in Indonesia, Thailand, and Costa Rica. The studies demonstrate the success of the constructed wetland systems in removing some pollutants of BOD, COD, TSS, pathogen, and detergent. The studies resulted in the treated water in a level of treatment that fulfils the requirement of the local standards for wastewater reuse as irrigation water, fishery, or other outdoor needs.

  12. Arsenic levels in the soils and macrophytes of the 'Entremuros' after the Aznalcollar mine spill

    Energy Technology Data Exchange (ETDEWEB)

    Taggart, M.A. [Department of Plant and Soil Science, University of Aberdeen, Cruickshank Building, Aberdeen AB24 3UU (United Kingdom)]. E-mail: mark.taggart@abdn.ac.uk; Carlisle, M. [Department of Geography and Environment, University of Aberdeen, Elphinstone Road, Aberdeen AB24 3UF (United Kingdom); Pain, D.J. [RSPB, The Lodge, Sandy, Bedfordshire SG19 2DL (United Kingdom); Williams, R. [RSPB, The Lodge, Sandy, Bedfordshire SG19 2DL (United Kingdom); Green, D. [Department of Geography and Environment, University of Aberdeen, Elphinstone Road, Aberdeen AB24 3UF (United Kingdom); Osborn, D. [Centre for Ecology and Hydrology, Monks Wood, Abbots Ripton, Huntingdon, Cambridgeshire PE28 2LS (United Kingdom); Meharg, A.A. [Department of Plant and Soil Science, University of Aberdeen, Cruickshank Building, Aberdeen AB24 3UU (United Kingdom)

    2005-01-01

    In April 1998, a holding lagoon containing pyrite ore processing waste rich in arsenic, failed and released 5-6 million m{sup 3} of highly polluting sludge and acidic water. Over 2700 ha of the internationally important Don-tilde ana National and Natural Parks were contaminated. The area of Natural Park to sustain the greatest impact was known as the Entremuros. This paper presents 0-5 cm soil monitoring data from the Entremuros, from sampling campaigns 6 and 18 months after the disaster; as well as macrophyte root, rhizome and stem data from samples taken 18 months after the spill. Results show a clear, decreasing, north-south arsenic soil pollution trend, both 6 and 18 months after the spill, and suggest a small reduction in total soil arsenic levels occurred over time; although a significant increase in extractable arsenic is also noted. The two macrophytes (Typha dominguensis and Scirpus maritimus) studied herein are not accumulating arsenic in stem parts, however, accumulation of arsenic on iron plaque on the roots of these plants may be occurring. Further work is recommended in order to determine the ecotoxicological significance of this process in relation to the avian food-chains of Don-tilde ana, and elsewhere. - Capsule: Arsenic associated with iron plaque on macrophyte roots may pose an ecotoxicological risk to certain herbivores.

  13. Tolerance and hyperaccumulation of a mixture of heavy metals (Cu, Pb, Hg, and Zn) by four aquatic macrophytes.

    Science.gov (United States)

    Romero-Hernández, Jorge Alberto; Amaya-Chávez, Araceli; Balderas-Hernández, Patricia; Roa-Morales, Gabriela; González-Rivas, Nelly; Balderas-Plata, Miguel Ángel

    2017-03-04

    In the present investigation, four macrophytes, namely Typha latifolia (L.), Lemna minor (L.), Eichhornia crassipes (Mart.) Solms-Laubach, and Myriophyllum aquaticum (Vell.) Verdc, were evaluated for their heavy metal (Cu, Pb, Hg, and Zn) hyperaccumulation potential under laboratory conditions. Tolerance analyses were performed for 7 days of exposure at five different treatments of the metals mixture (Cu(+2), Hg(+2), Pb(+2), and Zn(+2)). The production of chlorophyll and carotenoids was determined at the end of each treatment. L. minor revealed to be sensitive, because it did not survive in all the tested concentrations after 72 hours of exposure. E. crassipes and M. aquaticum displayed the highest tolerance to the metals mixture. For the most tolerant species of aquatic macrophytes, The removal kinetics of E. crassipes and M. aquaticum was carried out, using the following mixture of metals: Cu (0.5 mg/L) and Hg, Pb, and Zn 0.25 mg/L. The obtained results revealed that E. crassipes can remove 99.80% of Cu, 97.88% of Pb, 99.53% of Hg, and 94.37% of Zn. M. aquaticum withdraws 95.2% of Cu, 94.28% of Pb, 99.19% of Hg, and 91.91% of Zn. The obtained results suggest that these two species of macrophytes could be used for the phytoremediation of this mixture of heavy metals from the polluted water bodies.

  14. Integrated water quality, emergy and economic evaluation of three bioremediation treatment systems for eutrophic water

    Science.gov (United States)

    This study was targeted at finding one or more environmentally efficient, economically feasible and ecologically sustainable bioremediation treatment modes for eutrophic water. Three biological species, i.e. water spinach (Ipomoea aquatica), loach (Misgurus anguillicaudatus) and ...

  15. Yield and quality responses of drip-irrigated spinach to different irrigation quantities in a semi-arid region with a high altitude

    Directory of Open Access Journals (Sweden)

    Yasemin KUSLU

    2016-09-01

    Full Text Available The effect of different irrigation quantities on the growth, marketable yield, crop quality (antioxidant activity, mineral and total phenolic content and irrigation water use efficiency (IWUE of spinach grown in a semi-arid region of high altitude (1795 m a.s.l. was determined. Plants were irrigated with three different irrigation quantities which determined considering 100 (I1, 85 (I2 and 70% (I3 of the evaporation obtained from a Class A pan. Irrigation quantities in the I1, I2 and I3 treatments as two years average were 290, 264.3 and 238.6 mm, respectively. The I1 treatment provided the highest growth, marketable yield (28.06 t*ha-1 and IWUE (9.7 kg*m-3. However, mineral and total phenolic content and antioxidant activity in the leaves of spinach were significantly higher under lower water application conditions. The I2 treatment resulted with the highest antioxidant activity and content of N, K, Mg, Na, Fe, Cu and total phenolics. As a result of the study, I1 treatment in spinach production could be suitable for water sufficient regions due to higher yield and IWUE. However, I2 treatment may be more appropriate for water scarce semi-arid regions of high altitude for obtaining higher minerals and antioxidant activity.

  16. A Simplified Method for the Microscale Extraction of Pigments from Spinach

    Science.gov (United States)

    Cousins, Kimberley R.; Pierson, Kathleen M.

    1998-10-01

    A method is presented for microscale sample preparation for the thin-layer chromatographic analysis of the pigments in spinach. A commercial vegetable juicer is used for the initial extraction from spinach. This is followed by filtration, liquid/liquid extraction, centrifugation, and evaporation. The new sample preparation technique is significantly more efficient than present methods and minimizes solvents used for extraction.

  17. Development of a breeding strategy for nitrogen use efficiency in spinach (Spinacia oleracea L.)

    NARCIS (Netherlands)

    Chan Navarrete, J.R.

    2016-01-01

    Spinach (Spinacia oleracea L.) is one of the most consumed leafy vegetables worldwide and it is considered to be highly nutritious. Spinach is a short-cycle leafy crop that has a high demand for nitrogen in order to rapidly come to a harvestable product that has the required dark green colour within

  18. Feruloyl oligosaccharides from cell walls of suspension-cultured spinach cells and sugar beet pulp.

    Science.gov (United States)

    Ishii, T

    1994-06-01

    Cell walls of suspension-cultured spinach cells and sugar beet pulp were separately hydrolyzed with Driselase. A feruloyl arabinobiose was isolated from both spinach cells and sugar beet. Four feruloyl oligosaccharides were obtained from sugar beet. The four oligosaccharides were characterized by NMR spectroscopy, methylation analysis and FAB-MS.

  19. Submerged macrophyte communities in the Forsmark area. Building of a GIS application as a tool for biomass estimations

    Energy Technology Data Exchange (ETDEWEB)

    Fredriksson, Ronny [Univ. of Kalmar (Sweden)

    2005-12-15

    The aim of this study was to compile the information from previous studies to produce a GIS application that both illustrates the distribution of different vegetation communities and also makes it possible to estimate the total biomass of the different vegetation communities and its associated fauna. The GIS application was created by means of the software Arc View 3.3 by Environmental Systems Research Institute, Inc. Distribution readings and quantitative data of submerged macrophyte communities and its associated fauna was obtained from studies by Kautsky et al. and by Borgiel. Information about the macrophyte distribution in Laangoersviken, located in the northern parts of Kallrigafjaerden, was obtained from a report by Upplandsstiftelsen. Information about water depth and bottom substrate was available as USGS DEM file, produced by Geological Survey of Sweden. Complementary data of the covering degree of submerged vegetation was obtained from a study using an under water video camera by Tobiasson. Quantitative data on macrophyte and faunal biomass were either obtained from the primary SKB data base SICADA or directly from reports. Samples were compiled and analysed according to dominating vegetation. The work was carried out as follows: Where information about the bottom substrate was available polygons were created by means of the substrate shape file and depth grid from Geological Survey of Sweden. The vegetation community and the covering degree on a certain depth and substrate combination were determined by compiled information from studies by Kautsky and by Borgiel. All observations from a certain bottom substrate were analysed to find the dominating vegetation within different depth ranges. After determining the dominating vegetation, the covering degrees of different macrophyte classes within each depth range were calculated as a mean of all readings. Areas without information about the bottom substrate, but still adjacent to areas included in the

  20. Effects of Greater flamingo Phoenicopterus ruber on macrophytes, chironimds and turbidity in natural marshes in Doñana, SW Spain

    OpenAIRE

    Rodríguez-Pérez, Héctor; Andy J Green

    2007-01-01

    Top predators and nutrient inputs are important factors determining the structure of freshwater aquatic systems. Greater flamingos (Phoenicopterus ruber) has been shown to destroy submerged macrophytes and may promote a switch from clear water to turbid water in the temporary marshes of Doñana National Park. We excluded flamingos from twenty 4x4 m plots distributed between two lucios (shallow seasonal lakes) within the marshes from February to July 2004. In July, we measured total suspended s...

  1. Physical and ionic characteristics in water soluble fraction (WSF) of ...

    African Journals Online (AJOL)

    SERVER

    2008-01-04

    Jan 4, 2008 ... Key words: Physical and ionic characteristics, heavy metals, water soluble fraction, crude oil and Azolla africana. ... impact on aquatic life (Camougis, 1981). Water ..... Fish, fisheries, aquatic macrophytes and water quality in.

  2. pH gradients in the diffusive boundary layer of subarctic macrophytes

    KAUST Repository

    Hendriks, Iris E.

    2017-06-20

    Highly productive macrophytes produce diurnal and seasonal cycles in CO concentrations modulated by metabolic activity, which cause discrepancies between pH in the bulk water and near seaweed blades, especially when entering the diffusion boundary layer (DBL). Calcifying epiphytic organisms living in this environment are therefore exposed to a different pH environment than that of the water column. To evaluate the actual pH environment on blade surfaces, we measured the thickness of the DBL and pH gradients within it for six subarctic macrophytes: Fucus vesiculosus, Ascophyllum nodosum, Ulva lactuca, Zostera marina, Saccharina longicruris, and Agarum clathratum. We measured pH under laboratory conditions at ambient temperatures (2–3 °C) and slow, stable flow over the blade surface at five light intensities (dark, 30, 50, 100 and 200 µmol photons m s). Boundary layer thickness ranged between 511 and 1632 µm, while the maximum difference in pH (∆pH) between the blade surface and the water column ranged between 0.4 ± 0.14 (average ± SE; Zostera) and 1.2 ± 0.13 (average ± SE; Ulva) pH units. These differences in pH are larger than predictions for pH changes in the bulk water by the end of the century. A simple quadratic model best described the relationship between light intensity and maximum ∆pH, pointing at relatively low optimum PAR of between 28 and 139 µmol photons m s to reach maximum ∆pH. Elevated pH at the blade surface may provide chemical “refugia” for calcifying epiphytic organisms, especially during summer at higher latitudes where photoperiods are long.

  3. The importance of deciduous forest for alkalinity, phosphorus burial and isoetid macrophytes as revealed by a recent paleo study in a soft water Lobelia Lake (Grane Langsø, Denmark)

    DEFF Research Database (Denmark)

    Klamt, Anna-Marie; Reitzel, Kasper; Mortensen, Morten F.;

    in macrofossils of calcium (Ca)-sensitive species (as reflected in Daphnia ephippia and Cristatella mucedo statoblasts) and followed by increases in acidophilic species (as reflected in Juncus seeds and Sphagnum leaves). 2) The contents of organic material and organic P forms were clearly lower in sediments...... that were deposited during the period of least forest cover. Especially humic bound P seems to be positively related to the presence of deciduous trees which may be linked to a stabilizing effect of calcium. 3) An erosion event (as reflected in Cenococccum geophilum sclerotia) during the open-land period...... of nutrient-poor soft water lakes to changes in their catchments vegetation and their partial irreversible consequences and is therefore of importance for lake management....

  4. Efftcts of Two Aquatic Macrophytes on Phytoremediation to Severe Eutrophic Water%两种水生植物对重富营养化水体修复效果研究

    Institute of Scientific and Technical Information of China (English)

    常会庆; 王世华; 徐晓峰; 何俊瑜

    2012-01-01

    为了探索重富营养化水体的植物修复方法,在人工模拟条件下选取了两种水生植物伊乐藻(Elodeanuttallii)和黄花水龙(JussiaeastipulaceaOhwi),采用漂浮生长方式对富营养化水体中养分的去除效果进行研究。结果表明:沉水植物伊乐藻在水面漂浮条件下可起到净化水体的作用,对总氮、氨态氮、硝态氮、总磷、COD、叶绿素a的去除率分别为62.9%,90.83%,53.90%,71.21%,43.33%,50.05%;漂浮植物黄花水龙对上述污染物的去除效果分别为93.56%,97.38%,99.44%,97.74%,52.49%,58.34%。变异分析表明,两者对这些养分的去除达到了显著水平(P〈O.05)。黄花水龙对上述污染物的去除效果明显好于漂浮培养条件下的伊乐藻,适合重富营养化水体中养分的去除,而伊乐藻则在水质维持方面发挥更大作用。%In order to find a feasible phytoremediation to restore severe eutrophtc water, Lwu vq phytes Elodea nuttallii and Jussiaea stipulacea Ohwi were employed in floating artificial eutrophic water to get insight into differences involved in nutrient removal during incubation periods. The results illuminated that the removal percentages of total nitrogen (TN), ammonium (NHa-N), nitrate, phosphorus (TP), COD and Chlorophyll byElodeanuttallii were 62.9%, 90.83%, 53.90%, 71.21%, 43.33% and 50.05%, respectively, Corresponding peeentages for Jussiaea stipulacea OhvA were 93. 56%, 97, 38%, 99.44%, 97. 74%, 52.49% and 58.34%, respectively. Analysis of variance revealed difference of removel effects be tween two aquatic maerophytes was significant (P〈0.05). So Jussiaea stiptdacea Ohvoi is suited to restore severe eutrophic water because of its strong nutrient removal ability, while Etodea nuttallii can play great role in maintaining the water quality.

  5. Localization of phosphatidylcholine in outer envelope membrane of spinach chloroplasts

    Science.gov (United States)

    1985-01-01

    We have examined the effects of phospholipase C from Bacillus cereus on the extent of phospholipid hydrolysis in envelope membrane vesicles and in intact chloroplasts. When isolated envelope vesicles were incubated in presence of phospholipase C, phosphatidylcholine and phosphatidylglycerol, but not phosphatidylinositol, were totally converted into diacylglycerol if they were available to the enzyme (i.e., when the vesicles were sonicated in presence of phospholipase C). These experiments demonstrate that phospholipase C can be used to probe the availability of phosphatidylcholine and phosphatidylglycerol in the cytosolic leaflet of the outer envelope membrane from spinach chloroplasts. When isolated, purified, intact chloroplasts were incubated with low amounts of phospholipase C (0.3 U/mg chlorophyll) under very mild conditions (12 degrees C for 1 min), greater than 80% of phosphatidylcholine molecules and almost none of phosphatidylglycerol molecules were hydrolyzed. Since we have also demonstrated, by using several different methods (phase-contrast and electron microscopy, immunochemical and electrophoretic analyses) that isolated spinach chloroplasts, and especially their outer envelope membrane, remained intact after mild treatment with phospholipase C, we can conclude that there is a marked asymmetric distribution of phospholipids across the outer envelope membrane of spinach chloroplasts. Phosphatidylcholine, the major polar lipid of the outer envelope membrane, is almost entirely accessible from the cytosolic side of the membrane and therefore is probably localized in the outer leaflet of the outer envelope bilayer. On the contrary, phosphatidylglycerol, the major polar lipid in the inner envelope membrane and the thylakoids, is probably not accessible to phospholipase C from the cytosol and therefore is probably localized mostly in the inner leaflet of the outer envelope membrane and in the other chloroplast membranes. PMID:3988805

  6. Temporal and habitat distribution of macrophytes in lowland eutrophic reservoir Gruža in Serbia

    OpenAIRE

    Topuzović, Marina; Pavlović, Dragana; Ostojić, Aleksandar

    2016-01-01

    Background and Purpose: The research in this paper are based on the study of the distribution in time and space of macrophytes along the different parts of shoreline of Gruža Reservoir and emphasize the importance of establishing relations between distribution, diversity and mass of macrophytes and the ecological conditions of the environment. Development diversity of macrophytes needs to be constantly monitored and their regulation is necessary for Reservoir conservation, especially because...

  7. Temporal and spatial distribution of macrophytes in the Gruža Reservoir (Serbia)

    OpenAIRE

    Topuzović Marina; Pavlović Dragana; Ostojić A.

    2009-01-01

    A survey of aquatic macrophytes in the Gruža Reservoir (Serbia) was conducted during the period 2007-2008. Detailed data on the actual condition of macrophytes were established and compared between four parts of the reservoir. The distributions of most aquatic macrophytes of the Gruža Reservoir are heterogeneous, with different values of the MMT and MMO indices and low values of the distribution ratio. Quantitative analysis indicates that the greatest diversity and abundance of species can be...

  8. Temporal and habitat distribution of macrophytes in lowland eutrophic reservoir Gruža in Serbia

    OpenAIRE

    Topuzović, Marina; Pavlović, Dragana; Ostojić, Aleksandar

    2016-01-01

    Background and Purpose: The research in this paper are based on the study of the distribution in time and space of macrophytes along the different parts of shoreline of Gruža Reservoir and emphasize the importance of establishing relations between distribution, diversity and mass of macrophytes and the ecological conditions of the environment. Development diversity of macrophytes needs to be constantly monitored and their regulation is necessary for Reservoir conservation, especially because ...

  9. Aquatic macrophyte diversity of the Pantanal wetland and upper basin.

    Science.gov (United States)

    Pott, V J; Pott, A; Lima, L C P; Moreira, S N; Oliveira, A K M

    2011-04-01

    This is a short review of the state of the art concerning diversity of aquatic macrophytes and the main aquatic vegetation types in the Brazilian Pantanal wetland and upper watershed. There are ca. 280 species of aquatic macrophytes on the Pantanal floodplain, with scarce endemism. On the upper watershed, Cerrado wetlands (veredas) and limestone springs have a distinct flora from the Pantanal, with twice the species richness. As a representative case of aquatic habitats influenced by river flood, some primary data are presented for the Pantanal Matogrossense National Park and associated Acurizal Preserve, analysing the floristic similarity among aquatic vegetation types. We comment on problems of conservation and observe that Panicum elephantipes Nees is one of the few natives to compete with the invasive Urochloa arrecta (Hack. ex T. Durand & Schinz) Morrone & Zuloaga.

  10. Aquatic macrophyte diversity of the Pantanal wetland and upper basin

    Directory of Open Access Journals (Sweden)

    VJ. Pott

    Full Text Available This is a short review of the state of the art concerning diversity of aquatic macrophytes and the main aquatic vegetation types in the Brazilian Pantanal wetland and upper watershed. There are ca. 280 species of aquatic macrophytes on the Pantanal floodplain, with scarce endemism. On the upper watershed, Cerrado wetlands (veredas and limestone springs have a distinct flora from the Pantanal, with twice the species richness. As a representative case of aquatic habitats influenced by river flood, some primary data are presented for the Pantanal Matogrossense National Park and associated Acurizal Preserve, analysing the floristic similarity among aquatic vegetation types. We comment on problems of conservation and observe that Panicum elephantipes Nees is one of the few natives to compete with the invasive Urochloa arrecta (Hack. ex T. Durand & Schinz Morrone & Zuloaga.

  11. Bioactivity effect of two macrophyte extracts on growth performance of two bloom-forming cyanophytes

    Directory of Open Access Journals (Sweden)

    M.G. Ghobrial

    2015-01-01

    Full Text Available Allelopathy is a biological phenomenon by which an organism produces one or more biochemicals that influence the growth, survival, and reproduction of other organisms. These biochemicals are known as allelochemicals and can have beneficial (positive allelopathy or detrimental (negative allelopathy effects on the target organisms. The current research aims at using selected brackish water adapted submerged aquatic macrophytes allelopathy to combat bloom-forming cyanophytes, in laboratory bioassay experiments. Dry matters of macrophytes were extracted in solvents and the initial cyanophytes inoculum, derived from unialgal culture media, was used. Therefore, aqueous extracts with 50% and 100% acetone and ethanol solvents of two freshwater macrophytes; Potamogeton pectinatus and Ceratophyllum demersum were used to test their growth performance exhibited on two bloom-forming cyanophytes, Microcystis aeruginosa and Oscillatoria tenuis. The results revealed insignificant difference between the overall total average growth performance at treatment with 50% and 100% Ceratophyllum acetone extracts expressed by optical density (OD as well as chlorophyll a (chl a. Results showed, also, stimulation of M. aeruginosa growth. The highest growth increase in 100 μl/100 ml treatment with 50% acetone extract had a percentage rate (R of 94.66. On the contrary, treatment with ethanol extract recorded the highest inhibitory effect, thus in 1.5 μl/100 ml treatment with 50% Ceratophyllum ethanol extract R recorded −87.54, sustaining LC50 value of 1.12 μl/100 ml. The highest stimulating effect in 105 μl/100 ml treatment with 50% Ceratophyllum acetone extracts against O. tenuis was; R, 169.4. The highest inhibition in 1500 μl/100 ml treatment with 50% Ceratophyllum ethanol extracts against O. tenuis was; R −74.32, with LC50 0.830 μl/100 ml. While, the highest inhibition by 50% and 100% Potamogeton acetone or ethanol extracts against M. aeruginosa was

  12. Aquatic macrophyte community varies in urban reservoirs with different degrees of eutrophication

    Directory of Open Access Journals (Sweden)

    Suelen Cristina Alves da Silva

    2014-06-01

    Full Text Available AIM: Investigate spatial and temporal variation in the aquatic macrophyte community in four urban reservoirs located in Curitiba metropolitan region, Brazil. We tested the hypothesis that aquatic macrophyte community differ among reservoirs with different degrees of eutrophication. METHODS: The reservoirs selected ranged from oligotrophic/mesotrophic to eutrophic. Sampling occurred in October 2011, January 2012 and June 2012. Twelve aquatic macrophytes stands were sampled at each reservoir. Species were identified and the relative abundance of aquatic macrophytes was estimated. Differences among reservoirs and over sampling periods were analyzed: i through two‑way ANOVAs considering the stand extent (m and the stand biodiversity - species richness, evenness, Shannon-Wiener index and beta diversity (species variation along the aquatic macrophyte stand; and ii through PERMANOVA considering species composition. Indicator species that were characteristic for each reservoir were also identified. RESULTS: The aquatic macrophyte stand extent varied among reservoirs and over sampling periods. Species richness showed only temporal variation. On the other hand, evenness and Shannon-Wiener index varied only among reservoirs. The beta diversity of macrophyte stands did not vary among reservoirs or over time, meaning that species variability among aquatic macrophyte stands was independent of the stand extent and reservoir eutrophication. Community composition depended on the reservoir and sampling period. CONCLUSIONS: Our results support our initial expectation that reservoirs of different degrees of eutrophication have different aquatic macrophyte communities. As a consequence, each reservoir had particular indicator species. Therefore, monitoring and management efforts must be offered for each reservoir individually.

  13. Patterns of the aquatic macrophyte cover in Cachoeira Dourada Reservoir (GO-MG

    Directory of Open Access Journals (Sweden)

    L. M Bini

    Full Text Available The relationship between the aquatic macrophyte cover in upper segments of tributaries and this cover in these tributaries but near the reservoir's main body was tested. Sixteen taxa belonging to 12 families of aquatic macrophytes were recorded in Cachoeira Dourada Reservoir. The most frequent species were Eichhornia azurea (frequency of occurrence = 92%; n = 37 sites and E. crassipes (44%. Upper segments of the tributaries were the main areas colonized by these aquatic macrophytes. The positive relationship between the aquatic macrophyte cover between the upper and lower segments of tributaries indicates the importance of dispersion in the colonization of the arms and the reservoir's main body.

  14. Patterns of the aquatic macrophyte cover in Cachoeira Dourada Reservoir (GO-MG).

    Science.gov (United States)

    Bini, L M; Oliveira, L G; Souza, D C; Carvalho, P; Pinto, M P

    2005-02-01

    The relationship between the aquatic macrophyte cover in upper segments of tributaries and this cover in these tributaries but near the reservoir's main body was tested. Sixteen taxa belonging to 12 families of aquatic macrophytes were recorded in Cachoeira Dourada Reservoir. The most frequent species were Eichhornia azurea (frequency of occurrence=92%; n=37 sites) and E. crassipes (44%). Upper segments of the tributaries were the main areas colonized by these aquatic macrophytes. The positive relationship between the aquatic macrophyte cover between the upper and lower segments of tributaries indicates the importance of dispersion in the colonization of the arms and the reservoir's main body.

  15. Effect of submerged, freshwater aquatic macrophytes and littoral sediments on pan evaporation in the Lake Balaton region, Hungary

    Science.gov (United States)

    Anda, A.; Simon, B.; Soos, G.; Teixeira da Silva, J. A.; Kucserka, T.

    2016-11-01

    The evaporation (Ep) of a US Class A pan (C) with submerged, freshwater aquatic macrophytes (Potamogeton perfoliatus, Myriophyllum spicatum and Najas marina), hereafter macrophytes (Ps) and a sediment-covered bottom (S) was measured in Hungary during 2014-2015 using reference E of Shuttleworth (Eo) and Penman-Monteith crop reference evapotranspiration (crop ETo). There were two main climatic controls affecting variation in E: direct (air and water temperature) and indirect (wind-mediated change affecting the penetration of sunlight; precipitation inflow, impacting plant emergence). Lower seasonal mean Ep rates of 2.75 ± 0.89, 2.83 ± 0.91 and 3.06 ± 1.14 mm day-1 were observed in C, S and Ps, respectively, during the wet 2014. In the 2015 season, higher overall daily mean Ep rates for C, S and Ps were 3.76 ± 1.3, 4.19 ± 1.34 and 4.65 ± 1.52 mm day-1, respectively. A comparison of US Class A pan Ep containing macrophytes/sediments with that of a standard US Class A pan showed that pan coefficients (Kap and Kas) might allow for more accurate on-site lake E estimates. In 2014, seasonal mean Kas and Kap were 1.04 ± 0.14 and 1.09 ± 0.18, respectively. Slightly higher Ka values were observed during the warm and dry 2015 (Kas: 1.15 ± 0.22; Kap: 1.26 ± 0.23). A Ka value greater than 1 indicates that the Ep of a US Class A pan containing macrophytes and sediment is always higher than that of C. The calculated Eo overestimated measured Ep of Ps during the course of this study. During the warm-dry growing season, crop ETo was closest to Ep of Ps. Empirical coefficients can be useful for estimating E of lakes with submerged macrophytes more precisely. The accuracy of the estimate of Keszthely Bay's E improved by 9.85% when Ka was determined on site.

  16. Arsenic rich iron plaque on macrophyte roots - an ecotoxicological risk?

    Energy Technology Data Exchange (ETDEWEB)

    Taggart, M.A. [School of Biological Sciences, University of Aberdeen, Cruickshank Bld, St Machar Drive, Aberdeen, AB24 3UU (United Kingdom); Instituto de Investigacion en Recursos Cinegeticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real (Spain)], E-mail: mark.taggart@uclm.es; Mateo, R. [Instituto de Investigacion en Recursos Cinegeticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13005 Ciudad Real (Spain); Charnock, J.M.; Bahrami, F. [Synchrotron Radiation Department, CCLRC Daresbury Laboratory, Warrington, Cheshire, WA4 4AD (United Kingdom); Green, A.J. [Department of Wetland Ecology, Estacion Biologica de Donana, CSIC, Pabellon del Peru, Avenida Maria Luisa s/n, 41013 Seville (Spain); Meharg, A.A. [School of Biological Sciences, University of Aberdeen, Cruickshank Bld, St Machar Drive, Aberdeen, AB24 3UU (United Kingdom)

    2009-03-15

    Arsenic is known to accumulate with iron plaque on macrophyte roots. Three to four years after the Aznalcollar mine spill (Spain), residual arsenic contamination left in seasonal wetland habitats has been identified in this form by scanning electron microscopy. Total digestion has determined arsenic concentrations in thoroughly washed 'root + plaque' material in excess of 1000 mg kg{sup -1}, and further analysis using X-ray absorption spectroscopy suggests arsenic exists as both arsenate and arsenite. Certain herbivorous species feed on rhizomes and bulbs of macrophytes in a wide range of global environments, and the ecotoxicological impact of consuming arsenic rich iron plaque associated with such food items remains to be quantified. Here, greylag geese which feed on Scirpus maritimus rhizome and bulb material in areas affected by the Aznalcollar spill are shown to have elevated levels of arsenic in their feces, which may originate from arsenic rich iron plaque. - Accumulation of metals with iron plaque on macrophyte roots in wetlands poses an ecotoxicological risk to certain herbivores.

  17. Microphyte and macrophyte-based lagooning in tropical regions.

    Science.gov (United States)

    Noumsi, I M K; Nya, J; Akoa, A; Eteme, R A; Ndikefor, A; Fonkou, T; Brissaud, F

    2005-01-01

    A 720 m2 plant made of 8 ponds in series, set in Yaounde (Cameroon), was successively operated as a macrophyte-based system (type M) from November 1997 to October 98, a microphyte-based system (type m) from October 1999 to September 2000 and a combination of macrophyte and microphyte ponds (type M + m) from May to July 2001. Average applied loads varied over the years; from 420 kg. BOD5 ha(-1)d(-1) on the year 1997/98, the loads reached 510 kg BOD5 ha(-1)d(-1) in 1999/2000 and 500 in 2001. Though the system became more and more overloaded and sludge accumulated rapidly in the first ponds, it provided average removals of SS, BOD5 and COD that were always higher than 90% whatever the type of lagooning. Performances in the removal of SS, organic matter and the abatement of N-NH4+ and PO4(3-) did not significantly differ according to the type of lagooning and the applied load. Macrophyte lagooning did not show any definitive superiority as to nutrient removal when compared to microphyte lagooning. Microphyte lagooning was the most effective process in faecal indicators removal.

  18. 蕹菜镉积累品种根际土壤水溶性有机质的结构特征研究%Structural Characteristics of Dissolved Organic Matter in the Rhizosphere Soil of Water Spinach Cultivars Differing in Cd Accumulation

    Institute of Scientific and Technical Information of China (English)

    龚玉莲; 杨中艺; 陈爱葵; 曾小龙; 曾碧健; 王平洁; 汤域巍

    2015-01-01

    Fourier transform infrared spectroscopy (FTIR)and nuclear magnetic resonance spectroscopy (1 H NMR and 13 C NMR)were used to investigate the structural characteristics of dissolved organic mat-ter (DOM)in the rhizosphere soil of a low-Cd cultivar (QLQ)and a high-Cd cultivar (T308)of water spinach (Ipomoea aquatica Forsk.).The results showed that carbohydrates were the main components of DOMin the rhizosphere soil of the two cultivars,and the contents of alkyl and aromatic substances were relatively low.There were obvious differences in the structural characteristics of DOM between the two cultivars.The relative contents of carboxylic C and long-chain aliphatic C of DOMin the rhizosphere soil of QLQ were lower than those of T308,but that of aromatic substance of QLQ was higher.Alkyl sub-stance of DOM in the rhizosphere soil of QLQ possessed the characteristics of relatively high content, short branched chain and high branched chain.Although carbohydrate H content of DOM in the rhizo-sphere soil of QLQ was lower than that of T308,carbohydrate C content was higher than that of T308, which suggested that there might be differences in the structure of carbohydrates of DOM in the rhizo-sphere soil between the cultivars.The results indicated that the structural characteristics of DOM in the rhizosphere soil of the low-Cd cultivar might influence Cd behavior and bioavailability,leading to relative-ly low ability to activate soil Cd,which would be associated with its low Cd accumulation.%采用 FTIR 和1 H NMR、13 C NMR 研究了蕹菜 Ipomoea aquatica Forsk.低 Cd 品种(QLQ)和高 Cd 品种(T308)根际土壤水溶性有机质(dissolved organic matter,DOM)的结构特征。结果显示蕹菜两个品种根际土壤DOM均以碳水化合物为主要组成物质,烷基链烃和芳香族物质较少。两个品种根际土壤 DOM的结构特征存在明显差异。QLQ 根际土壤 DOM中羧基 C 和长链脂肪 C 的相对含量较 T308低

  19. Spectroelectrochemistry of P700 in native photosystem I particles and diethyl ether-treated thylakoid membranes from spinach and Thermosynechococcus elongatus.

    Science.gov (United States)

    Zhang, Yanrong; Nakamura, Akimasa; Kuroiwa, Yoshinori; Kato, Yuki; Watanabe, Tadashi

    2008-04-02

    The redox potentials (E(composite function')) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3 mV. Stepwise removal of antenna pigments by ether treatment caused distinct shifts of the E( composite function') value with increasing degree of water saturation in ether; negatively from +471 to +428 mV for spinach, but positively from +423 to +436 mV for T. elongatus. Such a contrasting behavior is discussed by invoking the mode of action of ether on the microenvironments around P700.

  20. The effect of foliar nutrition of spinach (Spinacia oleracea L.) with magnesium salts and urea on gas exchange, leaf yield and quality

    OpenAIRE

    Edward Borowski; Sławomir Michałek

    2012-01-01

    In a pot experiment conducted in a phytotron, the effectiveness of foliar nutrition of spinach (Spinacia oleracea L.) with different magnesium salts with and without the addition of 0.5% CO(NH2)2 was studied. Magnesium was applied 3 times in the form of solutions of MgSO4 x 7H2O, Mg(NO3)2 x 6H2O, MgCl2 x 6H2O, C4H6O4Mg x 4H2O, compared to water as the control treatment. The obtained results showed that foliar feeding of spinach with inorganic magnesium salts was an efficient method for supple...

  1. Characteristics and degradation of carbon and phosphorus from aquatic macrophytes in lakes: Insights from solid-state {sup 13}C NMR and solution {sup 31}P NMR spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Shasha [College of Water Sciences, Beijing Normal University, Beijing 100875 (China); State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Zhu, Yuanrong, E-mail: zhuyuanrong07@mails.ucas.ac.cn [State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Meng, Wei, E-mail: mengwei@craes.org.cn [State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); He, Zhongqi [USDA-ARS Southern Regional Research Center, 1100 Robert E Lee Blvd, New Orleans, LA 70124 (United States); Feng, Weiying [College of Water Sciences, Beijing Normal University, Beijing 100875 (China); State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Zhang, Chen [State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Giesy, John P. [State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012 (China); Department of Biomedical and Veterinary Biosciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)

    2016-02-01

    Water extractable organic matter (WEOM) derived from macrophytes plays an important role in biogeochemical cycling of nutrients, including carbon (C), nitrogen (N) and phosphorus (P) in lakes. However, reports of their composition and degradation in natural waters are scarce. Therefore, compositions and degradation of WEOM derived from six aquatic macrophytes species of Tai Lake, China, were investigated by use of solid-state {sup 13}C NMR and solution {sup 31}P NMR spectroscopy. Carbohydrates were the predominant constituents of WEOM fractions, followed by carboxylic acid. Orthophosphate (ortho-P) was the dominant form of P (78.7% of total dissolved P) in the water extracts, followed by monoester P (mono-P) (20.6%) and little diester P (0.65%). The proportion of mono-P in total P species increased with the percentage of O-alkyl and O–C–O increasing in the WEOM, which is likely due to degradation and dissolution of biological membranes and RNA from aquatic plants. Whereas the proportion of mono-P decreased with alkyl-C, NCH/OCH{sub 3} and COO/N–C=O increasing, which may be owing to the insoluble compounds including C functional groups of alkyl-C, NCH/OCH{sub 3} and COO/N–C=O, such as aliphatic biopolymers, lignin and peptides. Based on the results of this study and information in the literature about water column and sediment, we propose that WEOM, dominated by polysaccharides, are the most labile and bioavailable component in debris of macrophytes. Additionally, these WEOMs would also be a potential source for bioavailable organic P (e.g., RNA, DNA and phytate) for lakes. - Highlights: • WEOM derived from aquatic macrophytes was characterized. • C and P in WEOM were characterized by solid {sup 13}C NMR and solution {sup 31}P NMR. • Degradation and transformation of macrophyte-derived C and P were investigated. • Macrophyte-derived WEOM are important source for bioavailable nutrients in lakes.

  2. "Respirasome"-like supercomplexes in green leaf mitochondria of spinach.

    Science.gov (United States)

    Krause, Frank; Reifschneider, Nicole H; Vocke, Dirk; Seelert, Holger; Rexroth, Sascha; Dencher, Norbert A

    2004-11-12

    Higher plant mitochondria have many unique features compared with their animal and fungal counterparts. This is to a large extent related to the close functional interdependence of mitochondria and chloroplasts, in which the two ATP-generating processes of oxidative phosphorylation and photosynthesis, respectively, take place. We show that digitonin treatment of mitochondria contaminated with chloroplasts from spinach (Spinacia oleracea) green leaves at two different buffer conditions, performed to solubilize oxidative phosphorylation supercomplexes, selectively extracts the mitochondrial membrane protein complexes and only low amounts of stroma thylakoid membrane proteins. By analysis of digitonin extracts from partially purified mitochondria of green leaves from spinach using blue and colorless native electrophoresis, we demonstrate for the first time that in green plant tissue a substantial proportion of the respiratory complex IV is assembled with complexes I and III into "respirasome"-like supercomplexes, previously observed in mammalian, fungal, and non-green plant mitochondria only. Thus, fundamental features of the supramolecular organization of the standard respiratory complexes I, III, and IV as a respirasome are conserved in all higher eukaryotes. Because the plant respiratory chain is highly branched possessing additional alternative enzymes, the functional implications of the occurrence of respiratory supercomplexes in plant mitochondria are discussed.

  3. Effect of photoperiod on gibberellin biosynthetic enzymes in spinach

    Energy Technology Data Exchange (ETDEWEB)

    Gilmour, S.J.; Bleecker, A.B.; Zeevaart, J.A.D.

    1986-04-01

    The photoperiodic control of stem elongation in spinach, a long day (LD) rosette plant, is mediated by gibberellins (GAs). The early 13-hydroxylated GA biosynthetic pathway from GA/sub 12/ to GA/sub 20/ operates in spinach: GA/sub 12/ ..-->.. GA/sub 53/ ..-->.. GA/sub 44/ ..-->.. GA/sub 19/ ..-->.. GA/sub 20/. Two enzymes of this pathway, those converting GA/sub 53/ to GA/sub 44/ (GA/sub 53/ oxidase) and GA/sub 19/ to GA/sub 20/ (GA/sub 19/ oxidase), are regulated by light. The enzyme converting GA/sub 44/ to GA/sub 19/ (GA/sub 44/ oxidase) is not light-regulated. In the light GA/sub 53/ and GA/sub 18/ oxidase activities are increased, therefore causing the GA biosynthetic pathway to be turned on. This leads to the production of an active GA in LD, which causes an increase in stem elongation. Two the enzymes, GA/sub 44/ and GA/sub 53/ oxidases, can be separated from one another by anion exchange HPLC. Estimates of the molecular weights of these two enzymes based on gel filtration HPLC will be reported.

  4. Distribution and abundance of submerged aquatic macrophytes in a reactor cooling reservoir

    Energy Technology Data Exchange (ETDEWEB)

    Grace, J.B.

    1977-08-01

    Measurements of ash-free dry weight were used to characterize the effects of a heated effluent on submerged macrophytes in a reactor cooling reservoir. The species which were most abundant during the summers of 1974 and 1975 were Myriophyllum spicatum L. and Eleocharis acicularis (L.) R. and S. Examination of the vertical distribution of the shoot biomass of Myriophyllum revealed that plants in heated areas grew closer to the water surface than plants in unheated areas. The biomass of the second most abundant species, Eleocharis acicularis, was less at 0.5 m depths in heated areas (more than 5C/sup 0/ warmer than unheated areas) than at equal depths in unheated areas. Species diversity was greater at heated locations because of a greater equitability (i.e., evenness of distribution of biomass) among species.

  5. The use of macrophyte-based systems for phosphorus removal: an overview of 25 years of research and operational results in Florida.

    Science.gov (United States)

    DeBusk, T A; Dierber, F E; Reddy, K R

    2001-01-01

    Phosphorus (P) removal from wastewaters and surface runoff using macrophyte-based systems (MBS) has been a topic of great interest in Florida for over 25 years. During this period, P removal by both treatment wetlands and floating aquatic macrophyte systems has been evaluated from both a research and operational standpoint. Several factors have contributed to the increased focus on the use of MBS for P removal. First, there exist no conventional technologies that can cost-effectively achieve the low outflow P concentrations required to protect the integrity of Florida's relatively pristine surface waters. Second, because MBSs typically provide some water storage, they can accommodate the wide ranges of flows typical for runoff sources such as agricultural drainage waters. Finally, many regions in Florida have sufficient area for deployment of the relatively land-intensive MBS technologies. The first P removal work in Florida was initiated in the mid-1970s, and involved pilot-scale research on domestic wastewater treatment by natural wetlands. Parallel studies were performed with managed (periodically harvested) floating plant systems (i.e., Eichhornia crassipes) for tertiary treatment. Since that time, the range of operational systems that have been deployed include emergent macrophyte-based and forested wetlands, managed floating plant systems, and submerged macrophyte-based systems. Waters treated by MBS include domestic effluents, agricultural runoff and eutrophic lake waters. Phosphorus removal targets for MBS in Florida have been as low as 10 microg/L. In this paper, we summarize research and operational results for MBS in Florida over the past 25 years.

  6. Rapid cloning and bioinformatic analysis of spinach Y chromosome-specific EST sequences

    Indian Academy of Sciences (India)

    Chuan-Liang Deng; Wei-Li Zhang; Ying Cao; Shao-Jing Wang; Shu-Fen Li; Wu-Jun Gao; Long-Dou Lu

    2015-12-01

    The genome of spinach single chromosome complement is about 1000 Mbp, which is the model material to study the molecular mechanisms of plant sex differentiation. The cytological study showed that the biggest spinach chromosome (chromosome 1) was taken as spinach sex chromosome. It had three alleles of sex-related , m and . Many researchers have been trying to clone the sex-determining genes and investigated the molecular mechanism of spinach sex differentiation. However, there are no successful cloned reports about these genes. A new technology combining chromosome microdissection with hybridization-specific amplification (HSA) was adopted. The spinach Y chromosome degenerate oligonucleotide primed-PCR (DOP-PCR) products were hybridized with cDNA of the male spinach flowers in florescence. The female spinach genome was taken as blocker and cDNA library specifically expressed in Y chromosome was constructed. Moreover, expressed sequence tag (EST) sequences in cDNA library were cloned, sequenced and bioinformatics was analysed. There were 63 valid EST sequences obtained in this study. The fragment size was between 53 and 486 bp. BLASTn homologous alignment indicated that 12 EST sequences had homologous sequences of nucleic acids, the rest were new sequences. BLASTx homologous alignment indicated that 16 EST sequences had homologous protein-encoding nucleic acid sequence. The spinach Y chromosome-specific EST sequences laid the foundation for cloning the functional genes, specifically expressed in spinach Y chromosome. Meanwhile, the establishment of the technology system in the research provided a reference for rapid cloning of other biological sex chromosome-specific EST sequences.

  7. Suitability of macrophytes for nutrient removal from surface flow constructed wetlands receiving secondary treated sewage effluent in Queensland, Australia.

    Science.gov (United States)

    Greenway, M

    2003-01-01

    From a botanical perspective the major difference between waste stabilisation ponds and wetlands is the dominance of algae or floating plants in the former and emergent plants in the latter. Algae, floating and submerged plants remove nutrients directly from the water column whereas emergent species remove nutrients from the sediment. Water depth is a crucial factor in determining which plant types will become established. Surface flow constructed wetlands offer the greatest potential to grow a wide variety of different types of macrophytes. In assessing the suitability of plant species for nutrient removal, consideration must be given not only to nutrient uptake for growth but also storage of nutrients as plant biomass. A survey of macrophytes in 15 surface flow constructed wetlands treating secondary effluent was conducted in Queensland; 63 native species and 14 introduced species were found. Emergent species have been able to tolerate deeper water than in their natural environment and permanent waterlogging. All species grew well in the higher nutrient enriched wastewater. Submerged, floating leaved-attached and free floating species had the highest tissue nutrient content, followed by aquatic creepers. All these species remove nutrients from the water column. Emergent species had lower nutrient content but a greater biomass and were therefore able to store more nutrients per unit area of wetland. In order to maximise the efficiency of constructed wetlands for nutrient removal, a range of species should be used. Native species should be selected in preference to introduced/exotic species.

  8. Competition between free-floating and submerged macrophytes in a future of climate change

    NARCIS (Netherlands)

    Netten, J.J.C.

    2011-01-01


    This research was about the asymmetric competition between free-floating and submerged macrophytes in shallow freshwater ecosystems. I studied the effect of climate change on the dominance of free-floating macrophytes in temperate regions. The research approach was a combination of outdoor me

  9. Competition between free-floating and submerged macrophytes in a future of climate change

    NARCIS (Netherlands)

    Netten, J.J.C.

    2011-01-01


    This research was about the asymmetric competition between free-floating and submerged macrophytes in shallow freshwater ecosystems. I studied the effect of climate change on the dominance of free-floating macrophytes in temperate regions. The research approach was a combination of outdoor

  10. Macrophyte species distribution, indices of biotic integrity and sampling intensity in isolated Florida marshes

    Science.gov (United States)

    This study tested macrophyte condition metrics calculated after decreasing the effort and area of sampling by 33% to 66%, as tested in 74 emergent isolated wetlands. Four belted transects from wetland edge to center were established and rooted macrophytes were identified. The eff...

  11. Invertebrate grazing during the regenerative phase affects the ultimate structure of macrophyte communities

    NARCIS (Netherlands)

    Elger, A.F.; Willby, N.; Cabello-Martinez, M.

    2009-01-01

    1. Although the biomass of freshwater macrophytes consumed by invertebrate herbivores (excluding crayfish) is usually low, there is growing evidence that invertebrates do exert a structuring effect on macrophyte communities. To explain this, we postulated that the effect of invertebrates may be conc

  12. Effects of the herbicide 2,4-D on the growth of nine aquatic macrophytes

    NARCIS (Netherlands)

    Belgers, J.D.M.; Lieverloo, van R.J.; Pas, van der L.J.T.; Brink, van den P.J.

    2007-01-01

    A study was conducted to determine the effect of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) on nine submersed macrophyte species. The first objective of the study was to investigate the sensitivity of various endpoints in macrophyte toxicity tests. A second objective was to investigate the

  13. Experimental Effects of Lime Application on Aquatic Macrophytes: 4. Growth Response of Three Species

    Science.gov (United States)

    2007-04-01

    James PURPOSE: This investigation examined the growth response of three macrophyte species ( Elodea canadensis, Stuckenia pectinata, and Vallisnaria...different macrophyte species. These hypotheses were tested using experimental mesocosms. METHODS: Elodea canadensis, Stuckenia pectinata, and...Snel, P. E. Zanstra, and R. J. Helder. 1982. The mechanism of bicarbonate assimilation by the polar leaves of Potamogeton and Elodea . CO2 concentrations

  14. Effects of temperature on decomposition of a potential nuisance species: the submerged aquatic macrophyte Egeria najas planchom (Hydrocharitaceae

    Directory of Open Access Journals (Sweden)

    P Carvalho

    Full Text Available Decomposition of aquatic plants is influenced by several biotic and abiotic factors. Among them, temperature plays an important role. Despite the increasing number of studies describing the effects of temperature on the decomposition of aquatic macrophytes, little attention has been given to the decay of submerged macrophytes. In this paper, we assessed the effect of temperature on weight loss and chemical composition of detritus of the submerged aquatic macrophyte Egeria najas Planchon (Hydrocharitaceae. Fresh plant material was maintained at 17ºC and 27ºC, in the dark, in incubation chambers. The overall decay process was best described by a linear model, with rates of 0.014 day-1 (R² = 94% and 0.045 day-1 (R² = 96% obtained at 17ºC and 27ºC, respectively. The analysis of covariance (ANCOVA indicated a significant difference between the decomposition rates at the two temperatures. The rapid breakdown of E. najas detritus, indicated by the decay coefficient, may be explained by its low content of resistant compounds such as cellulose and lignin. The variables analyzed in this study (pH, electrical conductivity, dissolved oxygen in the water and organic matter, total nitrogen and total phosphorus concentration in detritus showed accentuated responses at 27ºC. It is likely that the higher temperature increased microbial activity and, therefore, oxygen consumption in the water, consequently affecting the pH and the rate of ion and nutrient liberation into the aquatic ecosystem. Due to the rapid decomposition of E. najas at high temperatures, a small exportation is expected of this species from its stands to distant regions in tropical reservoirs, where it is considered a potential nuisance species.

  15. Quantitative and qualitative characteristics of dissolved organic matter from eight dominant aquatic macrophytes in Lake Dianchi, China.

    Science.gov (United States)

    Qu, Xiaoxia; Xie, Li; Lin, Ying; Bai, Yingchen; Zhu, Yuanrong; Xie, Fazhi; Giesy, John P; Wu, Fengchang

    2013-10-01

    The aim of this research was to determine and compare the quantitative and qualitative characteristics of dissolved organic matters (DOM) from eight aquatic macrophytes in a eutrophic lake. C, H, N, and P in ground dry leaves and C, N, and P in DOM of the species were determined, and C/N, C/P, C/H, DOC/C, TDN/N, TDP/P, DOC/TDN, and DOC/TDP were calculated. Chemical structures of the DOM were characterized by the use of multiple techniques including UV-visible, FT-IR, and (13)C CP/MAS spectra. The results showed subtle differences in quantity and quality of DOM among species and life-forms. Except oriental pepper which had a C/H of 0.7, C/H of all the other species was 0.6. C/N and C/P of ground leaves was 10.5-17.3 and 79.4-225.3, respectively, which were greater in floating and submerged species than in the others. Parrot feather also had a small C/P (102.8). DOC/C, TDN/N, and TDP/P were 7.6-16.8, 5.5-22.6, and 22.9-45.6 %, respectively. Except C/N in emergent and riparian species, C/N in the other species and C/P in all the species were lower in their DOM than in the ground leaves. DOM of the macrophytes had a SUVA254 value of 0.83-1.80. The FT-IR and (13)C NMR spectra indicated that the DOM mainly contained polysaccharides and/or amino acids/proteins. Percent of carbohydrates in the DOM was 37.3-66.5 % and was highest in parrot feather (66.5 %) and crofton weed (61.5 %). DOM of water hyacinth, water lettuce, and sago pondweed may have the greatest content of proteins. Aromaticity of the DOM was from 6.9 % in water lettuce to 17.8 % in oriental pepper. DOM of the macrophytes was also different in polarity and percent of Ar-OH. Distinguished characteristics in quantity and quality of the macrophyte-derived DOM may induce unique environmental consequences in the lake systems.

  16. Carbonic anhydrase levels and internal lacunar CO/sub 2/ concentrations in aquatic macrophytes

    Energy Technology Data Exchange (ETDEWEB)

    Weaver, C.I.

    1979-01-01

    Carbonic anhydrase levels were examined in a variety of aquatic macrophytes from different habitats. In general, carbonic anhydrase levels increased across the habitat gradient such that activities were low in submersed aquatic macrophytes and high in emergent macrophytes with floating-leaved and free-floating plants exhibiting intermediate activities. Internal lacunar CO/sub 2/ concentrations were analyzed in relation to carbonic anhydrase activities. There was no correlation between these two parameters. Internal CO/sub 2/ concentrations ranged from low to high in submersed macrophytes, but were low in floating-leaved and emergent macrophytes. The observed internal CO/sub 2/ concentrations are discussed in relation to the individual morphologies of the plants and the environments in which they occurred.

  17. Effects of altered estuarine submerged macrophyte bed cover on the omnivorous Cape stumpnose Rhabdosargus holubi.

    Science.gov (United States)

    Sheppard, J N; Whitfield, A K; Cowley, P D; Hill, J M

    2012-03-01

    The ecological importance of submerged macrophyte beds to fishes within estuaries was investigated through the example of the ubiquitous Cape stumpnose Rhabdosargus holubi, an omnivorous, vegetation and estuary-dependent species, using stable-isotope techniques and long-term abundance (catch-per-unit-effort) data from the East Kleinemonde Estuary, South Africa. Outputs from a Bayesian mixing model using δ(13) C and δ(15) N signatures indicated that the submerged macrophytes Ruppia cirrhosa and Potamogeton pectinatus were not a primary source of nutrition for R. holubi, confirming previous work that revealed that macrophytes are consumed but not digested. Long-term seine netting data showed reduced abundance of R. holubi during a prolonged period of macrophyte senescence, suggesting that submerged macrophyte habitats provide shelter that reduces mortality (predation risk) and a food-rich foraging area. © 2012 SAIAB. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  18. Suppression Effects of Betaine-Enriched Spinach on Hyperhomocysteinemia Induced by Guanidinoacetic Acid and Choline Deficiency in Rats

    Directory of Open Access Journals (Sweden)

    Yi-Qun Liu

    2014-01-01

    Full Text Available Betaine is an important natural component of rich food sources, especially spinach. Rats were fed diets with betaine or spinach powder at the same level of betaine for 10 days to investigate the dose-dependent effects of spinach powder supplementation on hyperhomocysteinemia induced by guanidinoacetic acid (GAA addition and choline deprivation. The GAA-induced hyperhomocysteinemia in rats fed 25% casein diet (25C was significantly suppressed by supplementation with betaine or spinach, and it was completely suppressed by taking 11.0% spinach supplementation. The choline deprivation-induced enhancement of plasma homocysteine concentration in rats fed 25% soybean protein diet (25S was markedly suppressed by 3.82% spinach. Supplementation with betaine or spinach partially prevented the effects of GAA on hepatic concentrations of methionine metabolites. The decrease in activity of betaine-homocysteine S-methyltransferase (BHMT and cystathionine β-synthase (CBS in GAA-induced hyperhomocysteinemia was recovered by supplementation with betaine or spinach. Supplementation with betaine or spinach did not affect BHMT activity, whereas it partially restored CBS activity in choline-deprived 25S. The results indicated that betaine or spinach could completely suppress the hyperhomocysteinemia induced by choline deficiency resulting from stimulating the homocysteine removal by both remethylation and cystathionine formation.

  19. Phyllosphere bacterial community of floating macrophytes in paddy soil environments as revealed by illumina high-throughput sequencing.

    Science.gov (United States)

    Xie, Wan-Ying; Su, Jian-Qiang; Zhu, Yong-Guan

    2015-01-01

    The phyllosphere of floating macrophytes in paddy soil ecosystems, a unique habitat, may support large microbial communities but remains largely unknown. We took Wolffia australiana as a representative floating plant and investigated its phyllosphere bacterial community and the underlying driving forces of community modulation in paddy soil ecosystems using Illumina HiSeq 2000 platform-based 16S rRNA gene sequence analysis. The results showed that the phyllosphere of W. australiana harbored considerably rich communities of bacteria, with Proteobacteria and Bacteroidetes as the predominant phyla. The core microbiome in the phyllosphere contained genera such as Acidovorax, Asticcacaulis, Methylibium, and Methylophilus. Complexity of the phyllosphere bacterial communities in terms of class number and α-diversity was reduced compared to those in corresponding water and soil. Furthermore, the bacterial communities exhibited structures significantly different from those in water and soil. These findings and the following redundancy analysis (RDA) suggest that species sorting played an important role in the recruitment of bacterial species in the phyllosphere. The compositional structures of the phyllosphere bacterial communities were modulated predominantly by water physicochemical properties, while the initial soil bacterial communities had limited impact. Taken together, the findings from this study reveal the diversity and uniqueness of the phyllosphere bacterial communities associated with the floating macrophytes in paddy soil environments.

  20. Bryophytes and macro-algal growths as a part of macrophyte monitoring in rivers used for ecological assessment

    Directory of Open Access Journals (Sweden)

    Baláži P.

    2015-01-01

    Full Text Available Altogether, 62 taxa of macrophytes including 18 bryophytes and 16 macro-algal growths were determined at 87 survey sites (73 rivers representing the both ecoregions in Slovakia (Pannonian and Carpathian during the years 2010–2013. Bryophytes represented the dominant community in the Carpathians, while the occurrence of macro-algal growths was relatively balanced in both ecoregions. Ordination analyses (DCA showed an obvious shift within studied survey sites from vascular plants to bryophytes, while macro-algal growths were more or less uniform distributed in the whole ordination space. Based on stepwise (forward selection in CCA, altitude and water surfaces as a land use type were the main environmental factors responsible for this pattern and explained 13.7% of the variability. Variation partitioning showed that the shares of environmental variables on the total variation decreased in the following order: both groups together 8.3% (landscape and geographical variables, physicochemical variables, followed by landscape and geographical variables (5.8% and purely physicochemical variables which had an insignificant effect on macrophyte composition. The importance of both groups (bryophytes and macro-algal growths in ecological assessment was also confirmed by their contribution to the mean IBMR value determined for each water body type. Anyway, our study showed that their contribution to ecological assessment is not focused only on small mountain streams where they are dominant. They may obviously affect ecological assessment also in many water body types in lowland rivers and large upland rivers as well.

  1. Nuisance Aquatic Macrophyte Growth in the Northwest,

    Science.gov (United States)

    1980-12-12

    caused by 8 taxa: Elodea canadensis Michx. Potamogeton pectinatus L. Chara spp. Ranuculus ssp. Ceratophyllum demersum L Myriophyllum spp. Nitella spp...108 27 25.0 Elodea spp. (unidentified) (all data pooled) Eleocharis palustris 53 3 5.7 Zannichellia palustris so 7 14.0 S;cirus acutus 53 7 13.2 Sagit...GENERA IN DECREASING ORDER OF NUISANCE GROWTH EFFECT. Land Use Water-Body Type Nuisance Genera Population center Slough Elodea Grazing land Lake

  2. Selection of wild macrophytes for use in constructed wetlands for phytoremediation of contaminant mixtures.

    Science.gov (United States)

    Guittonny-Philippe, Anna; Petit, Marie-Eléonore; Masotti, Véronique; Monnier, Yogan; Malleret, Laure; Coulomb, Bruno; Combroux, Isabelle; Baumberger, Teddy; Viglione, Julien; Laffont-Schwob, Isabelle

    2015-01-01

    Constructed wetlands (CWs) offer an alternative to traditional industrial wastewater treatment systems that has been proved to be efficient, cost-effective and environmentally friendly. Most of the time, CWs are planted with proliferative species such as Phragmites australis or with plants originating from nurseries, both representing a risk for the natural biodiversity conservation of aquatic ecosystems located downstream of the CWs. For the removal of metals and organic pollutant mixtures present in industrial effluents, it is necessary to select tolerant plant species that are able to produce a high aboveground biomass and to develop a healthy belowground system. Wild plant species growing in aquatic bodies at industrial outfalls could constitute suitable tolerant species to use in CWs for industrial effluent treatment. To test this hypothesis, we assessed, under laboratory conditions (using an experimental design), the tolerance to mixtures of metals (Al, As, Cd, Cu, Cr, Fe, Mn, Ni, Pb, Sn, Zn) or/and organic pollutants (THC, PHE, PYR, LAS) of five European sub-cosmopolitan native macrophytes (Alisma lanceolatum, Carex cuprina, Epilobium hirsutum, Iris pseudacorus and Juncus inflexus) that had been collected in a polluted Mediterranean wetland, after a field study (crossing ecological relevés and analyses of contaminant concentrations in water and sediments). Our results demonstrated that research on phytoremediation of industrial effluents should focus much more on the use of native macrophytes growing at short distances from industrial discharges (such as C. cuprina in this study), and that root/shoot ratio, aerial height and proportion of green leaves are good and cost-effective indicators of plant tolerance to metals and organic pollutant mixtures in laboratory studies. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Role of macrophyte life forms in driving periphytic microalgal assemblages in a Brazilian reservoir

    Directory of Open Access Journals (Sweden)

    Ubirajara L. Fernandes

    2015-08-01

    Full Text Available Macrophytes play several roles in aquatic ecosystems, including the provision of habitat for many aquatic organisms, especially the periphyton. The aims of this study were to characterize the structure of periphytic microalgal assemblages on different aquatic macrophyte life forms in order to establish similarities between assemblages from nearby sampling sites. We hypothesized that i aquatic macrophytes with different life forms and morphological characteristics could differently influence the structure of the periphyton and that ii the greatest similarity in periphyton composition should be observed among macrophytes that occupy the same sampling site. The study was conducted from 2006 to 2008 in the Thomaz Osterne de Alencar reservoir (Crato City, Ceará State, Brazil and involved the taxonomic surveying of microalgae attached to five different macrophytes with the application of structural descriptors (richness, abundance, frequency, diversity and equitability. A total of 127 taxa, of which 44% belonged to the Chlorophyta, were identified. The microalgae assemblages showed high species richness on Salvinia auriculata Aubl., a free-floating macrophyte, and large abundance on Apalanthe granatensis (Humb. & Bonpl. Planch., a submerged anchored macrophyte. ANOVA indicated that periphyton significantly varied among the macrophytes investigated, and nearby sampling sites showed no structural similarities in microalgal assemblages. In general, we can conclude that the structure of periphyton assemblages is influenced by the substrate (i.e., macrophyte organ, as this can not only promote high diversity and equitability but can also be a predictor of dissimilarity in the distribution and frequency of occurrence of microalgae. These results reinforce the findings of other studies that have shown that macrophytes play an important role in structuring the periphyton assemblages.

  4. Submerged macrophytes mitigate direct and indirect insecticide effects in freshwater communities.

    Directory of Open Access Journals (Sweden)

    William R Brogan

    Full Text Available Understanding how ecological interactions mitigate the impacts of perturbations such as pesticides in biological communities is an important basic and applied question for ecologists. In aquatic ecosystems, new evidence from microcosm experiments suggests that submerged macrophytes can buffer cladocerans from pulse exposures to the widely used insecticide malathion, and that mitigation increases with macrophyte density. However, whether these results scale up to more complex aquatic communities where ecological interactions such as competition can alter toxicity is unknown. Further, macrophyte abilities to mitigate different insecticide exposure scenarios (i.e. single versus repeated pulses have never been tested. To address these gaps, we performed a factorial mesocosm experiment examining the influence of four macrophyte treatments (0, 10, 50, or 100 Elodea Canadensis shoots planted per mesocosm crossed with three malathion exposure scenarios (no insecticide, single pulse, repeated pulses on aquatic communities containing zooplankton, phytoplankton, periphyton, two snail species, and larval amphibians. In the absence of macrophytes, single malathion pulses caused short-term declines in cladoceran abundance followed by their rapid recovery, which precluded any indirect effects (i.e. trophic cascades. However, repeated malathion pulses caused cladoceran extinctions, resulting in persistent phytoplankton blooms and reduced abundance of one snail species. In contrast, with macrophytes present, even at low density, malathion had no effect on any taxa. We also discovered novel effects of macrophytes on the benthic food web. In the two highest macrophyte treatments, we observed trends of reduced periphyton biomass, decreased abundance of one snail species, and decreased amphibian time to and mass at metamorphosis. To our knowledge, this is the first evidence of negative submerged macrophyte effects on amphibians, a taxa of global conservation concern

  5. Solar energy from spinach and toothpaste: fabrication of a solar cell in schools

    Science.gov (United States)

    Siemsen, F.; Bunk, A.; Fischer, K.; Korneck, F.; Engel, H.; Roux, D.

    1998-01-01

    We will show how pupils can make a solar cell with spinach, toothpaste and a few other items found in any school laboratory. This device is called a Graetzel cell, and could trigger off a revolution in photovoltaic technology.

  6. In vitro liberation of carotenoids from spinach and Asia salads after different domestic kitchen procedures.

    Science.gov (United States)

    Eriksen, Jane N; Luu, Amy Y; Dragsted, Lars O; Arrigoni, Eva

    2016-07-15

    Green-leafy vegetables are rich in nutritionally important constituents including carotenoids. Their potential health benefits depend among others on their liberation from the plant matrix. The aim of the present study was to evaluate the effect of particle size and heat treatments on lutein and β-carotene liberation from spinach and Asia salads by applying an in vitro digestion protocol and UHPLC analysis. Reduction of particle size resulted in a three- to fourfold increase in liberation of lutein and β-carotene when comparing whole leaf and puree preparations of spinach. However, this positive effect was shown to be nullified by the severe heat impact during stir-frying of minced spinach, showing that domestic treatments need to be chosen carefully to maximise carotenoid liberation. Steaming significantly improved lutein liberation from Asia salads, but had no or a negative effect in spinach samples, possibly due to differences in liberation or degradation between the two plant matrices.

  7. Characterization of submerged macrophytes in a shallow lake using a combined approach of hyperspectral and LiDAR data

    Science.gov (United States)

    Valentini, E.; Bresciani, M.; Giardino, C.; Innocenti, C.; Taramelli, A.

    2009-12-01

    Aquatic vegetation is an important component of wetland ecosystems because of the key role in ecological functions of these environments. In the case of shallow lakes, such as the Trasimeno (central Italy), suspended sediment particles, frequently water level variation and algal bloom can lead to the reduction of underwater light penetration with feedback effect on submerged plants that sometimes drastically retreat. Macrophytes are an essential part of the productive zone in the Trasimeno lake and, by an equilibrium with algal growth, they contribute to the uptake of nutrients and pollutants, favour bottom quality, composition and stability and provide structural, food, shelter and breeding habitats for aquatic communities. The presence, abundance and species composition often represent a good limnological indicator of quality status of wetland areas. This study is a contribution on the knowledge of macrophytes of shallow lakes using a combined approach of airborne sensors such as MIVIS (Multi-spectral IR and Visible Imaging Spectrometer) and LiDAR (Light Detection and Ranging). The primary requirements for this ecosystem analysis are spatial and spectral resolution, accurate calibration and a suitable temporal baseline. Spectral Mixture Analysis (SMA) classifies individual mixed pixels according to the distribution of spectrally pure endmember fractions and provides a tool for discrimination and classification of wetlands areas. Physical and biological compositions are the primary determinants of the inherent reflectance properties at a landscape scale. We use SMA to discriminate among different types of reflectance properties on the basis of endmember fraction distributions. An in depth field validation has been also performed during the airborne survey that was on the 12 may 2009 and a spectral library of macrophytes has been collected. Field observations are necessary to calibrate and validate the spectral mixture model and subsequent thematic classification

  8. Acute Effects of a Spinach Extract Rich in Thylakoids on Satiety: A Randomized Controlled Crossover Trial

    OpenAIRE

    Rebello, Candida J.; Chu, Jessica; Beyl, Robbie; Edwall, Dan; Erlanson-Albertsson, Charlotte; Frank L. Greenway

    2015-01-01

    Objective: By retarding fat digestion, thylakoids, the internal photosynthetic membrane system of green plants, promote the release of satiety hormones. This study examined the effect of consuming a single dose of concentrated extract of thylakoids from spinach on satiety, food intake, lipids, and glucose compared to a placebo. Design: Sixty overweight and obese individuals enrolled in a double-blind randomized crossover study consumed the spinach extract or placebo in random order at least a...

  9. Reasoned opinion on the modification of the existing MRLs for thiacloprid in spinach and similar leaves

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2013-09-01

    Full Text Available In accordance with Article 6 of Regulation (EC No 396/2005, Belgium, hereafter referred to as the evaluating Member State (EMS, compiled an application to modify the existing MRLs for the active substance thiacloprid in spinach and similar leaves crop group. In order to accommodate the intended indoor and outdoor uses of thiacloprid in Belgium, the EMS proposed to raise the existing MRLs for thiacloprid in spinach and similar leaves group or only in spinach and beet leaves to 0.15 mg/kg. Belgium drafted an evaluation report in accordance with Article 8 of Regulation (EC No 396/2005, which was submitted to the European Commission and forwarded to EFSA. According to EFSA, the submitted residue trials data on lettuce are not sufficient to support the residue data extrapolation to the whole group of spinach and similar leaves. Alternatively, the EMS proposed to extrapolate four outdoor residue trials data on open leaf lettuce to spinach and beet leaves only. Although this extrapolation is not explicitly mentioned in the European guidance document, EFSA is of the opinion that the proposal is acceptable, considering the morphological similarities between spinach/beet leaves and open leave varieties of lettuce and taking into account that spinach and beet leaves are minor crops. Thus, a MRL proposal of 0.15 mg/kg is derived. Based on the risk assessment results EFSA concludes that the intended use of thiacloprid on spinach and beet leaves will not result in a consumer exposure exceeding the toxicological reference values and therefore is unlikely to pose a public health concern.

  10. Imaging metabolite dynamics in living cells using a Spinach-based riboswitch.

    Science.gov (United States)

    You, Mingxu; Litke, Jacob L; Jaffrey, Samie R

    2015-05-26

    Riboswitches are natural ligand-sensing RNAs typically that are found in the 5' UTRs of mRNA. Numerous classes of riboswitches have been discovered, enabling mRNA to be regulated by diverse and physiologically important cellular metabolites and small molecules. Here we describe Spinach riboswitches, a new class of genetically encoded metabolite sensor derived from naturally occurring riboswitches. Drawing upon the structural switching mechanism of natural riboswitches, we show that Spinach can be swapped for the expression platform of various riboswitches, allowing metabolite binding to induce Spinach fluorescence directly. In the case of the thiamine 5'-pyrophosphate (TPP) riboswitch from the Escherichia coli thiM gene encoding hydroxyethylthiazole kinase, we show that insertion of Spinach results in an RNA sensor that exhibits fluorescence upon binding TPP. This TPP Spinach riboswitch binds TPP with affinity and selectivity similar to that of the endogenous riboswitch and enables the discovery of agonists and antagonists of the TPP riboswitch using simple fluorescence readouts. Furthermore, expression of the TPP Spinach riboswitch in Escherichia coli enables live imaging of dynamic changes in intracellular TPP concentrations in individual cells. Additionally, we show that other riboswitches that use a structural mechanism similar to that of the TPP riboswitch, including the guanine and adenine riboswitches from the Bacillus subtilis xpt gene encoding xanthine phosphoribosyltransferase, and the S-adenosyl-methionine-I riboswitch from the B. subtilis yitJ gene encoding methionine synthase, can be converted into Spinach riboswitches. Thus, Spinach riboswitches constitute a novel class of RNA-based fluorescent metabolite sensors that exploit the diversity of naturally occurring ligand-binding riboswitches.

  11. Aquatic macrophytes of Itaipu Reservoir, Brazil: survey of species and ecological considerations

    Directory of Open Access Journals (Sweden)

    Sidinei Magela Thomaz

    1999-01-01

    Full Text Available In a survey of the aquatic macrophytes of the Itaipu Reservoir, we identified 62 taxa in 25 families and 42 genera. The highest number taxa was observed for the emergent macrophytes (40 taxa. Reduced fluctuation in water level, increased light penetration, and sediment enrichment by nutrients and organic matter following the formation of the reservoir favored the appearance of a species-rich community of submerged macrophytes (23% of the taxa identified. The aquatic macrophytes were found mainly near the mouths of the main tributaries of the reservoir, in shallow area of depth less than 2 meters. In addition to the shallow depth, the greater nutrient input from the tributaries and relative protection from wind explained this distribution. Among the species found, Egeria najas merits mention for its occurrence in all localities sampled, with biomass values varying between 98 and 186 gDW/m². Some potential nuisance species such as Eichhornia crassipes, Salvinia auriculata, and Pistia stratiotes also deserve attention, since they were also observed to be covering large areas of Itaipu Reservoir.Em um levantamento de macrófitas aquáticas realizado no reservatório de Itaipu foram identificados 62 táxons pertencentes a 25 famílias e 42 gêneros. O maior número de táxons foi representado pelas macrófitas emergentes (40 táxons. O surgimento de uma rica comunidade de espécies submersas (23% dos táxons identificados parece ter sido favorecido pelas alterações decorrentes da formação dor reservatório, tais como redução da flutuação dos níveis de água, aumento da penetração de luz e enriquecimento do sedimento em nutrientes e matéria orgânica. As macrófitas aquáticas foram registradas principalmente próximo à foz dos tributários do reservatório, em áreas rasas (profundidade inferior a 2 metros. Além da reduzida profundidade, o maior aporte de nutrientes associado à proteção do vento explicam essa distribuição. Entre

  12. Evaluation of the antimicrobial activities of ultrasonicated spinach leaf extracts using RAPD markers and electron microscopy.

    Science.gov (United States)

    Altemimi, Ammar; Lakhssassi, Naoufal; Abu-Ghazaleh, Amer; Lightfoot, David A

    2017-08-01

    Spinach (Spinacia oleracea L.) leaves represent an important dietary source of nutrients, antioxidants and antimicrobials. As such, spinach leaves play an important role in health and have been used in the treatment of human diseases since ancient times. Here, the aims were to optimize the extraction methods for recovering antimicrobial substances of spinach leaves, determine the minimum inhibitory concentrations (MICs) of the antimicrobial substances against Escherichia coli and Staphylococcus aureus and, finally, evaluate the effects of spinach leaves' antimicrobials on bacterial DNA using central composite face-centered methods. The effect of the extracts on both Gram-positive and Gram-negative bacterial models was examined by scanning electron microscopy (SEM) and random amplification of polymorphic (bacterial) DNA (RAPD). The optimal extraction conditions were at 45 °C, ultrasound power of 44% and an extraction time of 23 min. The spinach extracts exhibited antimicrobial activities against both bacteria with MICs in the 60-100 mg/ml range. Interestingly, SEM showed that the treated bacterial cells appear damaged with a reduction in cell number. RAPD analysis of genomic DNA showed that the number and sizes of amplicons were decreased by treatments. Based on these results, it was inferred that spinach leaf extracts exert bactericidal activities by both inducing mutations in DNA and causing cell wall disruptions.

  13. Newly developed SSR markers reveal genetic diversity and geographical clustering in spinach (Spinacia oleracea).

    Science.gov (United States)

    Göl, Şurhan; Göktay, Mehmet; Allmer, Jens; Doğanlar, Sami; Frary, Anne

    2017-08-01

    Spinach is a popular leafy green vegetable due to its nutritional composition. It contains high concentrations of vitamins A, E, C, and K, and folic acid. Development of genetic markers for spinach is important for diversity and breeding studies. In this work, Next Generation Sequencing (NGS) technology was used to develop genomic simple sequence repeat (SSR) markers. After cleaning and contig assembly, the sequence encompassed 2.5% of the 980 Mb spinach genome. The contigs were mined for SSRs. A total of 3852 SSRs were detected. Of these, 100 primer pairs were tested and 85% were found to yield clear, reproducible amplicons. These 85 markers were then applied to 48 spinach accessions from worldwide origins, resulting in 389 alleles with 89% polymorphism. The average gene diversity (GD) value of the markers (based on a GD calculation that ranges from 0 to 0.5) was 0.25. Our results demonstrated that the newly developed SSR markers are suitable for assessing genetic diversity and population structure of spinach germplasm. The markers also revealed clustering of the accessions based on geographical origin with clear separation of Far Eastern accessions which had the overall highest genetic diversity when compared with accessions from Persia, Turkey, Europe, and the USA. Thus, the SSR markers have good potential to provide valuable information for spinach breeding and germplasm management. Also they will be helpful for genome mapping and core collection establishment.

  14. Cerium relieving the inhibition of photosynthesis and growth of spinach caused by lead

    Institute of Scientific and Technical Information of China (English)

    ZHOU; Min

    2009-01-01

    Chloroplasts were isolated from spinach cultured in lead chloride-present, Ce3+-administered, cerium chloride-administered lead chloride-present Hoagland's media or that of Hoagland's media. The experimental study demonstrated the effects of cerium (Ce) on distribu-tion of light energy and photochemical activities of spinach chloroplast grown in lead (Pb)-present media. It was observed that Pb2+ signifi-cantly inhibited photosynthesis in spinach, including light absorption, energy transfer from LHCII to photosystem II, excitation energy dis-tribution from photosystem I to photosystem II, and transformation from light energy to electron energy and oxygen evolution of chloroplasts,and decreased spinach growth. However, Ce3+ treatment to pb2+-present chloroplasts could obviously improve light absorption and excitation energy distribution in both photosystems and increase activity of photochemical reaction and oxygen evolution of chloroplasts. The results suggested that Ce3+ under Pb2+ stress could maintain the stability of chloroplast membrane, and improve photosynthesis of spinach chloro-plast, thus promote spinach growth.

  15. Cerium relieving the inhibition of photosynthesis and growth of spinach caused by lead

    Institute of Scientific and Technical Information of China (English)

    ZHOU Min; ZE Yuguan; LI Na; DUAN Yanmei; CHEN Ting; LIU Chao; HONG Fashui

    2009-01-01

    Chloroplasts were isolated from spinach cultured in lead chloride-present, Ce~(3+)-administered, cerium chloride-administered lead chloride-present Hoagland's media or that of Hoagland's media. The experimental study demonstrated the effects of cerium (Ce) on distribu-tion of light energy and photochemical activities of spinach chloroplast grown in lead (Pb)-present media. It was observed that Pb~(2+) signifi-cantly inhibited photosynthesis in spinach, including light absorption, energy transfer from LHCII to photosystem II, excitation energy dis-tribution from photosystem I to photosystem II, and transformation from light energy to electron energy and oxygen evolution of chloroplasts,and decreased spinach growth. However, Ce~(3+) treatment to pb~(2+)-present chloroplasts could obviously improve light absorption and excitation energy distribution in both photosystems and increase activity of photochemical reaction and oxygen evolution of chloroplasts. The results suggested that Ce~(3+) under Pb~(2+) stress could maintain the stability of chloroplast membrane, and improve photosynthesis of spinach chloro-plast, thus promote spinach growth.

  16. Physico-chemical assessment of paper mill effluent and its heavy metal remediation using aquatic macrophytes--a case study at JK Paper mill, Rayagada, India.

    Science.gov (United States)

    Mishra, Swayamprabha; Mohanty, Monalisa; Pradhan, Chinmay; Patra, Hemanta Kumar; Das, Ritarani; Sahoo, Santilata

    2013-05-01

    The present investigation aims to assess the phytoremediation potential of six aquatic macrophytes, viz. Eichhornia crassipes, Hydrilla verticillata, Jussiaea repens, Lemna minor, Pistia stratiotes and Trapa natans grown in paper mill effluent of JK Paper mill of Rayagada, Orissa, for remediation of heavy metals. The experiment was designed in pot culture experiments. Assessment of physico-chemical parameters of paper mill effluent showed significant decrease in pH, conductivity, total dissolved solids, total suspended solids, chlorine, sulphur, biological and chemical oxygen demand after growth of macrophytes for 20 days. Phytoremediation ability of these aquatic macrophytic species for copper (Cu) and mercury (Hg) was indicated by assessing the decrease in the levels of heavy metals from effluent water. Maximum reduction (66.5 %) in Hg content of untreated paper mill effluent was observed using L. minor followed by T. natans (64.8 %). L. minor showed highest reduction (71.4 %) of Cu content from effluent water followed by E. crassipes (63.6 %). Phytoextraction potential of L. minor was remarkable for Hg and Cu, and bioaccumulation was evident from bioconcentration factor values, i.e. 0.59 and 0.70, respectively. The present phytoremediation approach was considered more effective than conventional chemical treatment method for removing toxic contaminants from paper mill effluent.

  17. Coexistence of fish species in a large lowland river: food niche partitioning between small-sized percids, cyprinids and sticklebacks in submersed macrophytes.

    Directory of Open Access Journals (Sweden)

    Małgorzata Dukowska

    Full Text Available In the spring and summer of each year, large patches of submersed aquatic macrophytes overgrow the bottom of the alluvial Warta River downstream of a large dam reservoir owing to water management practices. Environmental variables, macroinvertebrates (zoobenthos and epiphytic fauna, zooplankton and fish abundance and biomass were assessed at this biologically productive habitat to learn intraseasonal dynamics of food types, and their occurrence in the gut contents of small-sized roach, dace, perch, ruffe and three-spined stickleback. Gut fullness coefficient, niche breadth and niche overlap indicated how the fishes coexist in the macrophytes. Chironomidae dominated in the diet of the percids. However, ruffe consumed mostly benthic chironomids, while perch epiphytic chironomids and zooplankton. The diet of dace resembled that in fast flowing water although this rheophilic species occurred at unusual density there. The generalist roach displayed the lowest gut fullness coefficient values and widest niche breadth; consequently, intraspecific rather than interspecific competition decided the fate of roach. Three-spined stickleback differed from the other fishes by consuming epiphytic simuliids and fish eggs. The diet overlap between fishes reaching higher gut fullness coefficient values was rather low when the food associated with the submersed aquatic macrophytes was most abundant; this is congruent with the niche overlap hypothesis that maximal tolerable niche overlap can be higher in less intensely competitive conditions.

  18. Coexistence of fish species in a large lowland river: food niche partitioning between small-sized percids, cyprinids and sticklebacks in submersed macrophytes.

    Science.gov (United States)

    Dukowska, Małgorzata; Grzybkowska, Maria

    2014-01-01

    In the spring and summer of each year, large patches of submersed aquatic macrophytes overgrow the bottom of the alluvial Warta River downstream of a large dam reservoir owing to water management practices. Environmental variables, macroinvertebrates (zoobenthos and epiphytic fauna, zooplankton) and fish abundance and biomass were assessed at this biologically productive habitat to learn intraseasonal dynamics of food types, and their occurrence in the gut contents of small-sized roach, dace, perch, ruffe and three-spined stickleback. Gut fullness coefficient, niche breadth and niche overlap indicated how the fishes coexist in the macrophytes. Chironomidae dominated in the diet of the percids. However, ruffe consumed mostly benthic chironomids, while perch epiphytic chironomids and zooplankton. The diet of dace resembled that in fast flowing water although this rheophilic species occurred at unusual density there. The generalist roach displayed the lowest gut fullness coefficient values and widest niche breadth; consequently, intraspecific rather than interspecific competition decided the fate of roach. Three-spined stickleback differed from the other fishes by consuming epiphytic simuliids and fish eggs. The diet overlap between fishes reaching higher gut fullness coefficient values was rather low when the food associated with the submersed aquatic macrophytes was most abundant; this is congruent with the niche overlap hypothesis that maximal tolerable niche overlap can be higher in less intensely competitive conditions.

  19. Aquatic macrophytes in the large, sub-tropical Itaipu Reservoir, Brazil

    Directory of Open Access Journals (Sweden)

    Roger Paulo Mormul

    2010-12-01

    Full Text Available In the last three decades, rapid assessment surveys have become an important approach for measuring aquatic ecosystem biodiversity. These methods can be used to detect anthropogenic impacts and recognize local or global species extinctions. We present a floristic survey of the aquatic macrophytes along the Brazilian margin of the Itaipu Reservoir conducted in 2008 and compare this with a floristic survey conducted ten years earlier. We used ordination analysis to determine whether assemblage composition differed among reservoir arms. Macrophyte species were sampled in each of the 235 sampling stations using a boat, which was positioned inside three places of each macrophyte stand to record species and search for small plants. We also collected submerged plants using a rake with the boat moving at constant velocity for ten minutes. We assigned individual macrophyte species to life form and identified representative species for each life form. A total of 87 macrophyte taxa were identified. The "emergent" life forms contained the highest number of species, followed by "rooted submerged" life forms. The extensive survey of macrophytes undertaken in September 2008 recorded more species than a survey conducted between 1995 and 1998. This could be due to changes in water physico-chemistry, disturbances due to water drawdown and the long period between surveys, which may have allowed natural colonization by other species. Additionally, differences in the classification systems and taxonomic resolution used in the surveys may account for differences in the number of species recorded. Assemblage composition varied among the arms and was affected by underwater radiation (as measured using a Secchi disk and fetch. Five non-native species were found. Two of these non-native species (Urochloa subquadripara and Hydrilla verticillata are of special concern because they have a high frequency of occurrence and occupy large marginal areas of the reservoir. Future

  20. Differential photosynthetic and morphological adaptations to low light affect depth distribution of two submersed macrophytes in lakes

    Science.gov (United States)

    Chen, Jianfeng; Cao, Te; Zhang, Xiaolin; Xi, Yilong; Ni, Leyi; Jeppesen, Erik

    2016-01-01

    To evaluate the relative importance of photosynthetic versus morphological adaptations of submersed macrophytes to low light intensity in lakes, rapid light curves (RLCs), morphological parameters, relative growth rate (RGR), clonal reproduction and abundance of two submersed macrophytes (Potamogeton maackianus and Vallisneria natans) were examined under 2.8%, 7.1%, 17.1% and 39.5% ambient light in a field and outdoor experimental study. The plants increased their initial slope of RLCs (α) and decreased their minimum saturating irradiance (Ek) and maximum relative electron transport rate (ETRm) of RLCs under low light stress, but V. natans was more sensitive in RLCs than P. maackianus. Accordingly, the RGR, plant height and abundance of P. maackianus were higher in the high light regimes (shallow water) but lower in the low light regimes than those of V. natans. At the 2.8% ambient light, V. natans produced ramets and thus fulfilled its population expansion, in contrast to P. maackianus. The results revealed that P. maackianus as a canopy-former mainly elongated its shoot length towards the water surface to compensate for the low light conditions, however, it became limited in severe low light stress conditions. V. natans as a rosette adapted to low light stress mainly through photosynthetic adjustments and superior to severely low light than shoot elongation. PMID:27694880

  1. Water Quality Conditions at Tributary Projects in the Omaha District: 2008 Report

    Science.gov (United States)

    2009-01-01

    macrophytes ) and is consumed by aquatic plants, other biological organisms, and chemical oxidations. In reservoirs , the DO demand may be divided into two...in many reservoirs since fluctuating water levels may limit macrophyte and periphyton production. Phytoplankton can be generally grouped as diatoms...5 1.5.1 Reservoir Eutrophication and Hypolimnetic Dissolved Oxygen Depletion

  2. Inhibitory effect of monogalactosyldiacylglycerol, extracted from spinach using supercritical CO2, on mammalian DNA polymerase activity.

    Science.gov (United States)

    Iijima, Hiroshi; Musumi, Keiichi; Hada, Takahiko; Maeda, Naoki; Yonezawa, Yuko; Yoshida, Hiromi; Mizushina, Yoshiyuki

    2006-03-08

    We investigated the effective extraction of monogalactosyldiacylglycerol (MGDG) from dried spinach (Spinacia oleracea) using supercritical fluid carbon dioxide (SC-CO(2)) with a modifier/entrainer. The yield of MGDG in the SC-CO(2) extract was not influenced by increasing temperature at a constant pressure, although the total extract yield was decreased. The total extract yield and MGDG yield in the extract from commercially purchased spinach (unknown subspecies), were greatly influenced by lower pressure. In a modifier (i.e., ethanol) concentration range of 2.5-20%, both the extract and MGDG yield increased as the ethanol concentration rose. The highest total extract yield (69.5 mg/g of spinach) and a good MGDG yield (16.3 mg/g of spinach) were obtained at 80 degrees C, 25 MPa, and 20% ethanol. The highest MGDG concentration (76.0% in the extract) was obtained at 80 degrees C, 25 MPa, and 2.5% ethanol, although the total extract yield under these conditions was low (5.2 mg/g of spinach). The optimal conditions for the extraction of MGDG were 80 degrees C, 20 MPa, and 10% ethanol. Of the 11 subspecies of spinach tested under these conditions, "Ujyou" had the highest concentration of MGDG. The total extract yield and MGDG concentration of Ujyou were 20.4 mg of the extract/g of spinach and 70.5%, respectively. The concentration of MGDG was higher in the SC-CO(2) extract than in the extract obtained using solvents such as methanol and n-hexane. The extract of Ujyou, which was the optimal subspecies for the extraction of MGDG, inhibited the activity of calf DNA polymerase alpha with IC(50) values of 145 microg/mL but was not effective against DNA polymerase beta.

  3. Genetic diversity of spinach (Spinacia oleracea L. landraces collected in Iran using some morphological traits

    Directory of Open Access Journals (Sweden)

    Naser SABAGHNIA

    2015-12-01

    Full Text Available Spinach has become an important vegetable crop in most regions of the world and remarkable changes in production amounts have occurred in the past decades due to demand increase in many countries. Fifty-four spinach landraces collected from diverse geographical regions of Iran were evaluated for several qualitative and quantitative traits. Landraces indicated a high variability for measured morphologic characteristics regarding results of variance analysis and descriptive statistics. The first three factors of factors analysis explained 76.8% of variation of spinach landraces. The first extracted factor can be regarded as a leaf property vector; the extracted second factor could be named as yield vector and the third factor was female plants percent vector. The dendrogram of cluster analysis generated from genotypes distance matrices showed that in a distance linkage of 800, the 54 spinach landraces could be agglomerated into sixteen clusters. The number of clusters was verified by multivariate analysis of variance test through Wilks' Lambda statistics. Some spinach landraces such as G10 G13, G38 and G41 were individual cluster and were not similar to the other collected genotypes while some of the spinach landraces were similar to each other and grouped as one cluster such as cluster 9 (C9. The cluster C14 (landrace Karaj 2 was the most favorable genotype due to good performance for most measured quantitative traits. This landrace could be recommended for commercial release after complementary experiments. Also, landraces G1 (Arak and G3 (Urmia indicate good potential regarding the measured traits. These landraces could be used directly as commercial cultivars or introduced in spinach breeding programs.

  4. Inactivation of Escherichia coli O157:H7 attached to spinach harvester blade using bacteriophage.

    Science.gov (United States)

    Patel, Jitendra; Sharma, Manan; Millner, Patricia; Calaway, Todd; Singh, Manpreet

    2011-04-01

    Outbreaks associated with leafy greens have focused attention on the transfer of human pathogens to these commodities during harvest with commercial equipment. Attachment of Escherichia coli O157:H7 on new or rusty spinach harvester blades immersed in spinach extract or 10% tryptic soy broth (TSB) was investigated. Bacteriophages specific for E. coli O157:H7 were evaluated to kill cells attached to blade. A cocktail of five nalidixic acid-resistant E. coli O157:H7 isolates was transferred to 25 mL of spinach extract or 10% TSB. A piece of sterilized spinach harvester blade (2×1") was placed in above spinach extract or 10% TSB and incubated at room (22 °C) or dynamic (30 °C day, 20 °C night) temperatures. E. coli O157:H7 populations attached to blade during incubation in spinach extract or 10% TSB were determined. When inoculated at 1 log CFU/mL, E. coli O157:H7 attachment to blades after 24 and 48 h incubation at dynamic temperature (6.09 and 6.37 log CFU/mL) was significantly higher than when incubated at 22 °C (4.84 and 5.68 log CFU/mL), respectively. After 48 h incubation, two blades were sprayed on each side with a cocktail of E. coli O157-specific bacteriophages before scraping the blade, and subsequent plating on Sorbitol MacConkey media-nalidixic acid. Application of bacteriophages reduced E. coli O157:H7 populations by 4.5 log CFU on blades after 2 h of phage treatment. Our study demonstrates that E. coli O157:H7 can attach to and proliferate on spinach harvester blades under static and dynamic temperature conditions, and bacteriophages are able to reduce E. coli O157:H7 populations adhered to blades.

  5. Is nitrogen loading in wastewater more important than phosphorus? A historic review of the relationship between algae and macrophyte biomass and wastewater nutrient loading in the Bow River

    Science.gov (United States)

    Taube, Nadine; He, Jianxun; Ryan, Cathy; Valeo, Caterina

    2014-05-01

    The role of nutrient loading on biomass growth in wastewater-impacted rivers is important in understanding how to most effectively optimize wastewater treatment to avoid excessive biomass growth in the receiving water body. Nutrient loading is also affected by the nature of the effluent mixing in the river. This paper relates ammonium (NH4), nitrate (NO3) and total phosphorus (TP) from a wastewater treatment plant (WWTP) to epilithic algae and macrophyte biomass for determination of impacts of the WWTP on the Bow River ecosystem in Calgary, Alberta. Annual macrophyte biomass data and WWTP effluent nutrient data was analyzed for the years from 1981 - 2011. Locally Weighted Scatterplot Smoothing (LOWESS) was used to remove the influence of the river discharge from the biomass. The LOWESS method indicates that macrophytes do not grow beyond a maximum annual discharge of 300m3s-1. Algae biomass was most significantly correlated to daily mean discharge on sampling date and the LOWESS method indicates that they do not grow well beyond a daily mean discharge of 100m3s-1. Correlation analysis suggests that biomass in the Bow River is nitrogen limited. Epilithic algae are significantly correlated (p

  6. Decreasing but still significant facilitation effect of cold-season macrophytes on wetlands purification function during cold winter

    Science.gov (United States)

    Zou, Xiangxu; Zhang, Hui; Zuo, Jie; Wang, Penghe; Zhao, Dehua; An, Shuqing

    2016-06-01

    To identify the facilitation effect of a cool-season aquatic macrophyte (FEam) for use in effluent purification via constructed floating wetlands (CFWs) and to determine the possible pathways used during a winter period with an average temperature of less than 5 °C, pilot-scale CFWs were planted with the cold-season macrophyte Oenanthe clecumbens and were operated as batch systems. Although some leaves withered, the roots retained relatively high levels of activity during the winter, which had average air and water temperatures of 3.63 and 5.04 °C, respectively. The N and P removal efficiencies in CFWs decreased significantly in winter relative to those in late autumn. The presence of cool-season plants resulted in significant improvements in N and P removal, with a FEam of 15.23-25.86% in winter. Microbial N removal accounted for 71.57% of the total N removed in winter, and the decrease in plant uptake was the dominant factor in the wintertime decrease in N removal relative to that in late autumn. These results demonstrate the importance of cold-season plants in CFWs for the treatment of secondary effluent during cold winters.

  7. Decreasing but still significant facilitation effect of cold-season macrophytes on wetlands purification function during cold winter.

    Science.gov (United States)

    Zou, Xiangxu; Zhang, Hui; Zuo, Jie; Wang, Penghe; Zhao, Dehua; An, Shuqing

    2016-06-01

    To identify the facilitation effect of a cool-season aquatic macrophyte (FEam) for use in effluent purification via constructed floating wetlands (CFWs) and to determine the possible pathways used during a winter period with an average temperature of less than 5 °C, pilot-scale CFWs were planted with the cold-season macrophyte Oenanthe clecumbens and were operated as batch systems. Although some leaves withered, the roots retained relatively high levels of activity during the winter, which had average air and water temperatures of 3.63 and 5.04 °C, respectively. The N and P removal efficiencies in CFWs decreased significantly in winter relative to those in late autumn. The presence of cool-season plants resulted in significant improvements in N and P removal, with a FEam of 15.23-25.86% in winter. Microbial N removal accounted for 71.57% of the total N removed in winter, and the decrease in plant uptake was the dominant factor in the wintertime decrease in N removal relative to that in late autumn. These results demonstrate the importance of cold-season plants in CFWs for the treatment of secondary effluent during cold winters.

  8. Response of phytochelatins and their relationship with cadmium toxicity in a floating macrophyte Pistia stratiotes L. at environmentally relevant concentrations.

    Science.gov (United States)

    Wang, C; Wang, L Y; Sun, Q

    2010-02-01

    An indoor experiment was undertaken to investigate the response of phytochelatins and their relationship to cadmium toxicity in Pistia stratiotes L., a free-floating macrophyte, exposed to low concentrations of cadmium typically found in realistic environments. Cadmium concentrations of 0.01 to 0.08 microM had no toxic effects on the growth of this plant, as indicated by no significant changes in the fresh weights of leaves and roots and the slight induction of phytochelatins in plant tissues, whereas cadmium concentrations of 0.16 to 1 microM were toxic, and cadmium toxicity increased with the increase of cadmium concentrations in solutions, accompanied by the dramatic production of phytochelatins in plant tissues, especially in roots. There was a positive correction between root phytochelatin levels and cadmium toxicity, as measured by the growth inhibition rate of the root fresh weight. The results suggested that phytochelatins in aquatic macrophytes can serve as sensitive biomarkers for heavy metal toxicity in a moderately polluted water environment.

  9. Purification of gibberellin sub 53 -oxidase from spinach

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, T.M.; Zeevaart, J.A.D. (Michigan State Univ., East Lansing (USA))

    1989-04-01

    Spinach is a long-day rosette plants, in which stem growth is mediated by gibberellins. It has been shown that two enzymatic steps, GA{sub 53}-oxidase and GA{sub 19}-oxidase, are controlled by light. To develop an understanding into this light regulation, purification of GA{sub 53}-oxidase has been undertaken. The original assay relied on the HPLC separation of the product and substrate, but was considered too slow for the development of a purification scheme. A TLC system was developed which in conjunction with improvements to the assay conditions was sensitive and gave rapid results. The partial purification of the GA{sub 53}-oxidase is achieved by a high speed centrifugation, 40-55% ammonium sulfate precipitation, an hydroxyapatite column, Sephadex G-100 column and an anion exchange FPLC column, Mono Q HR10/10, yielding 1000-fold purification and 15% recovery. Monoclonal antibodies to the protein will be raised and used to further characterize the enzyme.

  10. [Fluorescence used to investigate the sensitivity of spinach chloroplast membrane to low intensity electromagnetic radiation].

    Science.gov (United States)

    Xi, Gang; Yang, Yun-Jing; Lu, Hong

    2009-07-01

    A system for studying biological effect of radio frequency electromagnetic field was developed. The system can form an area where electromagnetic wave with large frequency range is well distributed. The strength of electromagnetic wave was measured easily. Electromagnetic wave in the system did not have effect on environment. The sensitivity of spinach chloroplast membrane to low intensity electromagnetic radiation of 300 MHz under power density of 5 mW x cm(-2) was studied by the spectral analysis method of fluorescence of 8-anilino-1-naphthalene-sulfonic acid (ANS) and the changes in chlorophyll a (Chla) fluorescence parameters of spinach chloroplast membrane. The result showed that the position of spectrum of ANS fluorescence of spinach chloroplast membrane did not change, but the intensity of ANS fluorescence was obviously increased under the action of electromagnetic radiation with power density of 1-5 mW x cm(-2). There was an increase in the intensity of ANS fluorescence with the increase in electromagnetic radiation. The increase of ANS fluorescence of spinach chloroplast membrane showed that low level electromagnetic field induced the decrease in fluidity of chloroplast membrane compared with control experiment. The cause of the change in the fluidity could be related to the polarization of chloroplast membrane under the electromagnetic field. The analysis of Chla fluorescence parameters of spinach chloroplast membrane indicated that low level electromagnetic field of 300 MHz made the fluorescence parameters F0 and F(VI/)F(V) decrease, and F(V)/Fo, Fv/F(m) and deltaF(V)/T increase. It was showed that low level electromagnetic field caused the change of non-active center of photosystem II of spinach chloroplast membrane to active center and the increase in potential active and photochemical efficiency of PSII, and promoted the transmit process of electron in photosynthesis of chloroplast membrane of photosynthesis cell in spinach leaf. The study confirmed

  11. Interactions between nitrifying and denitrifying bacteria in gnotobiotic microcosms planted with the emergent macrophyte Glyceria maxima

    NARCIS (Netherlands)

    Bodelier, P.L.E.; Duyts, H.; Blom, C.W.P.M.; Laanbroek, H.J.

    1998-01-01

    The population dynamics of the chemolithoautotrophic nitrifiers Nitrosomonas europaea and Nitrobacter winogradskyi were studied in gnotobiotic microcosms fed with ammonium in response to the presence or absence of the emergent macrophyte Glyceria maxima and the heterotrophic denitrifying bacterium P

  12. Studies on heavy metal accumulation in aquatic macrophytes from Sevan (Armenia) and Carambolim (India) lake systems

    Digital Repository Service at National Institute of Oceanography (India)

    Vardanyan, L.G.; Ingole, B.S.

    in root system. The representative macrophytes from two different physiographic locations show similar trends and order in accumulating different metals generally. Of the 14 metals investigated, 9 (Ca, Fe, Al, Cr, Cu, Ba, Ti, Co and Pb) showed higher rates...

  13. Closely related freshwater macrophyte species, Ceratophyllum demersum and em>C. submersum, differ in temperature response

    DEFF Research Database (Denmark)

    Hyldgaard, Benita; Sorrell, Brian Keith; Brix, Hans

    2014-01-01

    1. The importance of temperature responses of photosynthesis and respiration in determining species distributions was compared in two closely related freshwater macrophytes, Ceratophyllum demersum and C. submersum. The two species differed significantly in response to temperature in the short and...

  14. Effects of litter on substrate conditions and growth of emergent macrophytes

    NARCIS (Netherlands)

    Van der Putten, W.H.; Peters, B.A.M.; Van den Berg, R.S.

    1997-01-01

    Three successive emergent macrophytes (Typha latifolia L., Phragmites australis (Cav.) Steudel and Glyceria maxima (Hartman) Holmbly) were each grown in substrates collected from three different zones of shoreline vegetation development (non- vegetated sediment, the interface between T. latifolia an

  15. Does mechanical disturbance affect the performance and species composition of submerged macrophyte communities?

    Science.gov (United States)

    Zhang, Qian; Xu, Ying-Shou; Huang, Lin; Xue, Wei; Sun, Gong-Qi; Zhang, Ming-Xiang; Yu, Fei-Hai

    2014-01-01

    Submerged macrophyte communities are frequently subjected to disturbance of various frequency and strength. However, there is still little experimental evidence on how mechanical disturbance affects the performance and species composition of such plant communities. In a greenhouse experiment, we constructed wetland communities consisting of five co-occurring clonal submerged macrophyte species (Hydrilla verticillata, Elodea canadensis, Ceratophyllum demersum, Chara fragilis, and Myriophyllum spicatum) and subjected these communities to three mechanical disturbance regimes (no, moderate and strong disturbance). Strong mechanical disturbance greatly decreased overall biomass, number of shoot nodes and total shoot length, and increased species diversity (evenness) of the total community. It also substantially decreased the growth of the most abundant species (H. verticillata), but did not affect growth of the other four species. Our data reveal that strong disturbance can have different effects on different submerged macrophyte species and thus alters the performance and species composition of submerged macrophyte communities. PMID:24811826

  16. Capabilities of Seven Species of Aquatic Macrophytes for Phytoremediation of Pentachlorophenol Contaminated Sediment

    Science.gov (United States)

    Zhao, Liangyuan; Guo, Weijie; Li, Qingyun; Li, Huan; Zhao, Weihua; Cao, Xiaohuan

    2017-01-01

    Sediments are regarded as the ultimate sink of pentachlorophenol(PCP) in aquatic environment, and capabilities of seven species of aquatic macrophytes for remediating PCP contaminated sediment were investigated. Seven species of aquatic macrophytes could significantly accelerate the degradation of PCP in sediments. Among all, canna indica L., Acorus calamus L. and Iris tectorum Maxim. can be used as efficient alternative plants for remediation of PCP contaminated sediment, which attained 98%, 92% and 88% of PCP removal in sediments, respectively. PCP was detected only in root tissues and the uptake was closely related to the root lipid contents of seven plants. The presence of seven aquatic macrophytes significantly increased microbial populations and the activities of dehydrogenase compared with control sediments, indicating that rhizosphere microorganism played important role in the remediation process. In conclusion, seven species of aquatic macrophytes may act as promising tools for the PCP phytoremediation in aquatic environment, especially Canna indica L., Acorus calamus L. and Iris tectorum Maxim.

  17. Principal Component Analysis of Some Quantitative and Qualitative Traits in Iranian Spinach Landraces

    Directory of Open Access Journals (Sweden)

    Mohebodini Mehdi

    2017-08-01

    Full Text Available Landraces of spinach in Iran have not been sufficiently characterised for their morpho-agronomic traits. Such characterisation would be helpful in the development of new genetically improved cultivars. In this study 54 spinach accessions collected from the major spinach growing areas of Iran were evaluated to determine their phenotypic diversity profile of spinach genotypes on the basis of 10 quantitative and 9 qualitative morpho-agronomic traits. High coefficients of variation were recorded in some quantitative traits (dry yield and leaf area and all of the qualitative traits. Using principal component analysis, the first four principal components with eigen-values more than 1 contributed 87% of the variability among accessions for quantitative traits, whereas the first four principal components with eigen-values more than 0.8 contributed 79% of the variability among accessions for qualitative traits. The most important relations observed on the first two principal components were a strong positive association between leaf width and petiole length; between leaf length and leaf numbers in flowering; and among fresh yield, dry yield and petiole diameter; a near zero correlation between days to flowering with leaf width and petiole length. Prickly seeds, high percentage of female plants, smooth leaf texture, high numbers of leaves at flowering, greygreen leaves, erect petiole attitude and long petiole length are important characters for spinach breeding programmes.

  18. Haloacetic acids in the aquatic environment. Part I: macrophyte toxicity.

    Science.gov (United States)

    Hanson, Mark L; Solomon, Keith R

    2004-08-01

    Haloacetic acids (HAAs) are contaminants of aquatic ecosystems with numerous sources, both anthropogenic and natural. The toxicity of HAAs to aquatic plants is generally uncharacterized. Laboratory tests were conducted with three macrophytes (Lemna gibba, Myriophyllum sibiricum and Myriophyllum spicatum) to assess the toxicity of five HAAs. Myriophyllum spp. has been proposed as required test species for pesticide registration in North America, but few studies have been conducted under standard test conditions. The HAAs in the present experiments were monochloroacetic acid (MCA), dichloroacetic acid (DCA), trichloroacetic acid (TCA), trifluoroacetic acid (TFA) and chlorodifluoroacetic acid (CDFA). MCA was the most toxic to Myriophyllum spp. with EC50 values ranging from 8 to 12.4 mg/l depending on the endpoint, followed by DCA (EC50 range 62-722.5 mg/l), TCA (EC50 range 49.5-1702.6 mg/l), CDFA (EC50 range 105.3 to >10,000 mg/l) and with TFA (EC50 range 222.1 to 10,000 mg/l) the least toxic. Generally, L. gibba was less sensitive to HAA toxicity than Myriophyllum spp., with the difference in toxicity between them approximately threefold. The range of toxicity within Myriophyllum spp. was normally less than twofold. Statistically, plant length and node number were the most sensitive endpoints as they had the lowest observed coefficients of variation, but they were not the most sensitive to HAA toxicity. Toxicological sensitivity of endpoints varied depending on the measure of effect chosen and the HAA, with morphological endpoints usually an order of magnitude more sensitive than pigments for all plant species. Overall, mass and root measures tended to be the most sensitive indicators of HAA toxicity. The data from this paper were subsequently used in an ecological risk assessment for HAAs and aquatic plants. The assessment found HAAs to be of low risk to aquatic macrophytes and the results are described in the second manuscript of this series.

  19. Experimental Effects of Lime Application on Aquatic Macrophytes: 3. Growth Response Versus Exposure Time

    Science.gov (United States)

    2008-01-01

    ERDC/TN APCRP-EA-17 January 2008 Experimental Effects of Lime Application on Aquatic Macrophytes : 3. Growth Response Versus Exposure Time by William...be effective in both suppressing submersed macrophyte growth and changing species composition (Babin et al. 1992; Chambers et al. 2001; Prepas et al...16 cm height) filled with homogenized sediment (obtained from Eau Galle Reservoir , WI; see James et al. (2005) for a description of sediment

  20. Predictors of occurrence of the aquatic macrophyte Podostemum ceratophyllum in a southern Appalachian River

    Science.gov (United States)

    Argentina, Jane E.; Freeman, Mary C.; Freeman, Byron J.

    2010-01-01

    The aquatic macrophyte Podostemum ceratophyllum (Hornleaf Riverweed) commonly provides habitat for invertebrates and fishes in flowing-water portions of Piedmont and Appalachian streams in the eastern US. We quantified variation in percent cover by P. ceratophyllum in a 39-km reach of the Conasauga River, TN and GA, to test the hypothesis that cover decreased with increasing non-forest land use. We estimated percent P. ceratophyllum cover in quadrats (0.09 m2) placed at random coordinates within 20 randomly selected shoals. We then used hierarchical logistic regression, in an information-theoretic framework, to evaluate relative support for models incorporating alternative combinations of microhabitat and shoal-level variables to predict the occurrence of high (≥50%)P. ceratophyllum cover. As expected, bed sediment size and measures of light availability (location in the center of the channel, canopy cover) were included in best-supported models and had similar estimated-effect sizes across models. Podostemum ceratophyllum cover declined with increasing watershed size (included in 8 of 13 models in the confidence set of models); however, this decrease in cover was not well predicted by variation in land use. Focused monitoring of temporal and spatial trends in status of P. ceratophyllum are important due to its biotic importance in fast-flowing waters and its potential sensitivity to landscape-level changes, such as declines in forested land cover and homogenization of benthic habitats.

  1. Feruloyl Oligosaccharides from Cell Walls of Suspension-Cultured Spinach Cells and Sugar Beet Pulp : STRUCTURE AND FUNCTION OF CELLS

    OpenAIRE

    Tadashi, Ishii; Forestry and Forest Products Research Institute

    1994-01-01

    Cell walls of suspension-cultured spinach cells and sugar beet pulp were separately hydrolyzed with Driselase. A feruloyl arabinobiose was isolated from both spinach cells and sugar beet. Four feruloyl oligosaccharides were obtained from sugar beet. The four oligosaccharides were characterized by NMR spectroscopy, methylation analysis and FAB-MS.

  2. THE INTERNAL RESISTANCE IN SPINACH LEAVES TO ATMOSPHERIC H2S DEPOSITION IS DETERMINED BY METABOLIC PROCESSES

    NARCIS (Netherlands)

    DE KOK, LJ; RENNENBERG, H; KUIPER, PJC

    1991-01-01

    Spinach (Spinacia oleracea) leaves formed an active sink for atmospheric H2S. Upon short-term exposure, H2S flux to the leaves showed saturation kinetics with respect to the H2S concentrations and could be described by the Michaelis-Menten equation. The kinetics of H2S flux to spinach leaves were

  3. The study of aquatic macrophytes in Neotropics: a scientometrical view of the main trends and gaps.

    Science.gov (United States)

    Padial, A A; Bini, L M; Thomaz, S M

    2008-11-01

    Aquatic macrophytes comprises a diverse group of organisms including angiosperms, ferns, mosses, liverworts and some macroalgae that occur in seasonally or permanently wet environments. Among other implications, aquatic macrophytes are highly productive and with an important structuring role on aquatic environments. Ecological studies involving aquatic plants substantially increased in the last years. However, a precise view of researches devoted to aquatic macrophytes in Neotropics is necessary to reach a reliable evaluation of the scientific production. In the current study, we performed a scientometrics analysis of the scientific production devoted to Neotropical macrophytes in an attempt to find the main trends and gaps of researches concerning this group. The publication devoted to macrophytes in Neotropics increased conspicuously in the last two decades. Brazil, Argentina, Mexico and Chile were the most productive among Neotropical countries. Our analyses showed that the studies dealt mostly with the influences of aquatic macrophytes on organisms and abiotic features. Studies with a predictive approach or aiming to test ecological hypothesis are scarce. In addition, researches aiming to describe unknown species are still necessary. This is essential to support conservation efforts and to subsidize further investigations testing ecological hypotheses.

  4. Growth rate, protein:RNA ratio and stoichiometric homeostasis of submerged macrophytes under eutrophication stress

    Directory of Open Access Journals (Sweden)

    Xing W.

    2016-01-01

    Full Text Available Growth rate hypothesis (GRH and stoichiometric homeostasis of photoautotrophs have always been questioned. However, little is known about GRH and stoichiometric homeostasis of aquatic plants, especially submerged macrophytes. Therefore, we aim to test the GRH and explore stoichiometric homeostasis of four freshwater submerged macrophytes under eutrophication stress. At the single species level and the multi-species level, N:P ratios of Potamogeton maackianus, Myriophyllum spicatum, Vallisneria natans and Ceratophyllum demersum had no consistent trends with growth rates. However, protein:RNA ratios of P. maackianus, M. spicatum and V. natans all correlated negatively with growth rates, demonstrating GRH can apply to freshwater submerged macrophytes, even though they are threatening by eutrophication stress. Protein:RNA ratios positively correlated with N:P ratios in culture media and tissues in submerged macrophytes except in P. maackianus (30d, suggesting effects of varying N:P ratios in culture media on protein:RNA ratios are basically in concert with tissue N:P ratios under short-time eutrophication stress. Stoichiometric homeostasis coefficients (HN:P indicated submerged macrophytes have weak homeostasis. Stoichiometric homeostasis of V. natans was stronger than those of P. maackianus, M. spicatum and C. demersum. The differences in GRH and homeostasis of the four submerged macrophytes may be due to species traits.

  5. Sediment phosphate composition in relation to emergent macrophytes in the Doñana Marshes (SW Spain)

    Science.gov (United States)

    Reina, M.; Espinar, J.L.; Serrano, L.

    2006-01-01

    We have studied the effect of the presence of emergent macrophytes on the sediment phosphate composition of a eutrophic shallow marsh on the NE margin of Doñana (SW Spain). Top sediment and water samples were collected from both the open-water and the vegetated sites at three areas covered by different plant species: Scirpus maritimus, Juncus subulatus and Phragmites australis. The concentration of organic matter was significantly higher in the top sediment of sites covered by vegetation than in their adjacent open-water sites at the three vegetation areas. The P-fractional composition showed that the sediment was dominated by the inorganic P-fractions in all cases, reaching the highest concentration in the Ca-bound P-fraction (281–372 μg g−1 d.w.). The sum of all P-fractions was significantly higher in the top sediment of the sites covered by J. subulatus and S. maritimus than in their adjacent open-water sites, and so were the org-P fraction extracted by hot NaOH and the concentration of phytate within this fraction. Deposition of plant material on the top sediment of areas vegetated by J. subulatus and S. maritimus explains these differences. The P-fractional composition of the seeds from J. subulatus showed that they contained a large proportion of organic P-fractions, particularly of the fraction extracted by hot NaOH (1868 μg g−1 d.w., 85% of which was phytate). The presence of emergent macrophytes, therefore, influenced the distribution of P-fractions in the sediment depending on plant species. The P-bioavailability of shallow aquatic systems must be fully understood if wetlands are to be protected from further eutrophication.

  6. Carotenoids and flavonoids in organically grown spinach (Spinacia oleracea L) genotypes after deep frozen storage

    DEFF Research Database (Denmark)

    Kidmose, U.; Knuthsen, Pia; Edelenbos, M.

    2001-01-01

    After frozen storage the content of individual carotenoids and flavonoids was determined in organically grown spinach genotypes (Spinacia oleracea L) which differed in leaf colour and shape. The spinach was sorted, washed, blanched in steam for 3 min and frozen in liquid nitrogen. After frozen...... storage the green colour was determined by sensory evaluation and HunterLab colorimetry. The content of individual chlorophylls, carotenoids and flavonoids was determined using HPLC. Lutein, beta -carotene, violaxanthin and 9 '-(Z)-neoxanthin were the main carotenoids in processed spinach. The total...... content of carotenoids varied from 176.6 mg kg(-1) 'wet weight' as eaten in the lightest green genotype to 226.3 mg kg(-1) 'wet weight' as eaten in the darkest green genotype. The highest content of beta -carotene (83.1 mg kg(-1) 'wet weight' as eaten) was found in the dark green genotype. The content...

  7. Carotenoids and flavonoids in organically grown spinach (Spinacia oleracea L) genotypes after deep frozen storage

    DEFF Research Database (Denmark)

    Kidmose, U.; Knuthsen, Pia; Edelenbos, M.

    2001-01-01

    After frozen storage the content of individual carotenoids and flavonoids was determined in organically grown spinach genotypes (Spinacia oleracea L) which differed in leaf colour and shape. The spinach was sorted, washed, blanched in steam for 3 min and frozen in liquid nitrogen. After frozen...... storage the green colour was determined by sensory evaluation and HunterLab colorimetry. The content of individual chlorophylls, carotenoids and flavonoids was determined using HPLC. Lutein, beta -carotene, violaxanthin and 9 '-(Z)-neoxanthin were the main carotenoids in processed spinach. The total...... content of carotenoids varied from 176.6 mg kg(-1) 'wet weight' as eaten in the lightest green genotype to 226.3 mg kg(-1) 'wet weight' as eaten in the darkest green genotype. The highest content of beta -carotene (83.1 mg kg(-1) 'wet weight' as eaten) was found in the dark green genotype. The content...

  8. Effects of Rare Earth Elements on Vigor Enhancement of Aged Spinach Seeds

    Institute of Scientific and Technical Information of China (English)

    刘超; 洪法水; 郑蕾; 汤萍; 王志刚

    2004-01-01

    The effect and the mechanism of action of lanthanum, cerium and neodymium on aged seeds of spinach were studied. By LaCl3, CeCl3, and NdCl3 treatment, the germination rate, germination index and vigor index of aged spinach seeds are increased and the activities of superoxide dismutase, catalase and peroxidase are enhanced. Moreover the ·O2- and malondialdehyde content are decreased and the cell membrane permeability of aged spinach seeds is reduced. Among these three rare earth elements, Ce treatment enhances vigor of aged seeds most significantly, that of Nd treatment secondly and La treatment is not as effective as the other two treatments. The reason may be from 4f electron characteristic and alternation valence of REEs.

  9. Effect of hexane extract of spinach in the removal of arsenic from rat

    Directory of Open Access Journals (Sweden)

    Badar Uddin Umar

    2007-03-01

    Full Text Available Extensive search is going on for a cheap, easily available and effective remedy of chronic arsenic poisoning. The present study was designed to find the effects of hexane extract of spinach in the removal of arsenic from arsenic treated rat. Rats were fed arsenic trioxide through Ryle’s tube for one month then they were fed on hexane extract (1-4% of spinach for another one month. Hexane extract of spinach decreased accumulated arsenic from rat liver, spleen, kidney, intestine, lungs and skin significantly. Besides, it reduced the oxidative stress caused by arsenic which was evident by decreased levels of malondialdehye (MDA in the above tissues. Hexane extract decreases both arsenic level and MDA level in rat tissues in dose dependent manner, which is more effective at lower doses.

  10. Effect of hexane extract of spinach in the removal of arsenic from rat

    Directory of Open Access Journals (Sweden)

    Badar Uddin Umar

    2007-06-01

    Full Text Available Extensive search is going on for a cheap, easily available and effective remedy of chronic arsenic poisoning. The present study was designed to find the effects of hexane extract of spinach in the removal of arsenic from arsenic treated rat. Rats were fed arsenic trioxide through Ryle’s tube for one month then they were fed on hexane extract (1-4% of spinach for another one month. Hexane extract of spinach decreased accumulated arsenic from rat liver, spleen, kidney, intestine, lungs and skin significantly. Besides, it reduced the oxidative stress caused by arsenic which was evident by decreased levels of malondialdehyde (MDA in the above tissues. Hexane extract decreases both arsenic level and MDA level in rat tissues in dose dependent manner, which is more effective at lower doses.

  11. Water Hyacinth Eichhornia crassipes (Mart. Solms-Laubach Dynamics and Succession in the Nyanza Gulf of Lake Victoria (East Africa: Implications for Water Quality and Biodiversity Conservation

    Directory of Open Access Journals (Sweden)

    John Gichuki

    2012-01-01

    Full Text Available This study, conducted in Nyanza Gulf of Lake Victoria, assessed ecological succession and dynamic status of water hyacinth. Results show that water hyacinth is the genesis of macrophyte succession. On establishment, water hyacinth mats are first invaded by native emergent macrophytes, Ipomoea aquatica Forsk., and Enydra fluctuans Lour., during early stages of succession. This is followed by hippo grass Vossia cuspidata (Roxb. Griff. in mid- and late stages whose population peaks during climax stages of succession with concomitant decrease in water hyacinth biomass. Hippo grass depends on water hyacinth for buoyancy, anchorage, and nutrients. The study concludes that macrophyte succession alters aquatic biodiversity and that, since water hyacinth infestation and attendant succession are a symptom of broader watershed management and pollution problems, aquatic macrophyte control should include reduction of nutrient loads and implementing multifaceted approach that incorporates biological agents, mechanical/manual control with utilization of harvested weed for cottage industry by local communities.

  12. Natural variation of folate content and composition in spinach (Spinacia oleracea) germplasm.

    Science.gov (United States)

    Shohag, M J I; Wei, Yan-yan; Yu, Ning; Zhang, Jie; Wang, Kai; Patring, Johan; He, Zhen-li; Yang, Xiao-e

    2011-12-14

    Breeding to increase folate levels in edible parts of plants, termed folate biofortification, is an economical approach to fight against folate deficiency in humans, especially in the developing world. Germplasm with elevated folates are a useful genetic source for both breeding and direct use. Spinach is one of the well-know vegetables that contains a relatively high amount of folate. Currently, little is known about how much folate, and their composition varies in different spinach accessions. The aim of this study was to investigate natural variation in the folate content and composition of spinach genotypes grown under controlled environmental conditions. The folate content and composition in 67 spinach accessions were collected from the United States Department of Agriculture (USDA) and Asian Vegetable Research and Development Center (AVRDC) germplasm collections according to their origin, grown under control conditions to screen for natural diversity. Folates were extracted by a monoenzyme treatment and analyzed by a validated liquid chromatography (LC) method. The total folate content ranged from 54.1 to 173.2 μg/100 g of fresh weight, with 3.2-fold variation, and was accession-dependent. Four spinach accessions (PI 499372, NSL 6095, PI 261787, and TOT7337-B) have been identified as enriched folate content over 150 μg/100 g of fresh weight. The folate forms found were H(4)-folate, 5-CH(3)-H(4)-folate, and 5-HCO-H(4)-folate, and 10-CHO-folic acid also varied among different accessions and was responsible for variation in the total folate content. The major folate vitamer was represented by 5-CH(3)-H(4)-folate, which on average accounted for up to 52% of the total folate pool. The large variation in the total folate content and composition in diverse spinach accessions demonstrates the great genetic potential of diverse genotypes to be exploited by plant breeders.

  13. Biplot analysis of trait relations of spinach (Spinacia oleracea L. landraces

    Directory of Open Access Journals (Sweden)

    Sabaghnia Naser

    2016-01-01

    Full Text Available Interest in growing winter spinach (Spinacia oleracea L. in Iran is increasing due to its good nutritional potential returns relative to other vegetable crops. The objectives of this research were to investigate the interrelationships among different traits of spinach and to evaluate different Iranian spinach landraces with application of the genotype × trait (GT biplot methodology in visualizing research data. 81 spinach landraces were grown during 2-years according to randomized complete block design with four replications. Ranking of the genotypes based on the ideal entry revealed that genotypes G1, G20, G7, G8, G9, G27, G49 G70 and G79 were higher in the measured traits and could be good candidates for improving most of the measured traits. Ranking of traits for the leaf yield showed that petiole diameter, petiole length, leaf numbers at flowering, 1000-seed weight and root dry weight were the most discriminating traits which influence spinach leaf yield at both years. There were 9 winning genotypes and 4 which-won-where patterns at the first year while there were 8 winning genotypes and 4 which-won-where patterns at the second year. As a result, the findings from our study are as follows: (i traits leaf numbers at flowering, leaf length, leaf width, leaf area, petiole diameter and petiole length could be as selection indices for spinach leaf yield improvement, (ii genotypes G1, G20, G7, G8, G9, G27, G49 G70, and G79 were the most favorable and is thus recommended for commercial release or incorporating in breeding programs; (iii the GT biplot method can be used to identify superior genotypes in other crops and in other parts of the world.

  14. Starch accumulation during hydroponic growth of spinach and basil plants under carbon dioxide enrichment

    Energy Technology Data Exchange (ETDEWEB)

    Holbrook, G.P.; Hansen, J.; Wallick, K.; Zinnen, T.M. (North Illinois University, de Kalb, IL (USA). Dept. of Biological Sciences)

    1993-04-01

    The effects of CO[sub 2] enrichment, photoperiod duration, and inorganic phosphate levels on growth and starch accumulaton by spinach and basil plants were studied in a commercial hydroponic facility. During a 3-week growth period, both species exhibited increased whole-plant fresh weight as a result of an increase in atmospheric CO[sub 2] concentration from 400 to 1500 mul/1. However, basil leaves exhibited a 1.5- to 2-fold greater increase in specific leaf weight (SLW), and accumulated starch to much greater levels than did leaves of spinach. At 1500 mul CO[sub 2]/1, starch accounted for up to 38% of SLW with basil compared to [lt] 10% of SLW with spinach. The maximum ratio of starch/chlorophyll was 55.0 in basil leaves vs 8.0 in spinach leaves. High ratio values were associated with the appearance of chlorotic symptoms in leaves of basil grown under CO[sub 2] enrichment, whereas spinach did not exhibit chlorosis. Increasing inorganic phosphate concentrations from 0.7 to 1.8 mM in the hydroponic medium did not appreciably affect leaf starch accumulation in either species. Starch accumulation in basil leaves was not consistently related to the duration of the photoperiod. However, photoperiod-induced changes in leaf starch levels were much greater in basil than spinach. The results clearly indicate that different horticultural crops can show diverse responses to CO[sub 2] enrichment, and thus highlight the need to develop individual growth strategies to optimize production quality of each species.

  15. The ichthyofauna of drifting macrophyte mats in the Ivinhema River, upper Paraná River basin, Brazil

    Directory of Open Access Journals (Sweden)

    Cíntia Karen Bulla

    2011-06-01

    Full Text Available We describe the fish assemblages associated with drifting macrophyte mats and consider their possible role as dispersal vectors in the Ivinhema River, a major tributary of the upper Paraná River, Brazil. Fish associated with drifting mats were sampled in the main river channel during January and March 2005, when the wind and/or the increased water level were sufficient to transport macrophyte stands. Fish in the drifting mats were sampled with a floating sieve (4 m long x 2 m wide x 0.6 m high, and 2 mm mesh size. In the laboratory, larvae, juvenile, and adult fish were counted and identified to the lowest possible taxonomic level. In four drifting macrophyte mats we captured 218 individuals belonging to at least 28 species, 17 families, and 6 orders. Aphyocharax dentatus, Serrasalmus spp., and Trachelyopterus galeatus were the most abundant taxa associated with the mats, but species richness ranged from 6 to 24 species per mat. In addition, 85% of the total number of individuals caught was larvae and juveniles. Although preliminary and based on limited samples, this study of drifting macrophyte mats was the first one in the last unregulated stretch of the Paraná River remaining inside Brazilian territory, and alerts us to the potential role of macrophytes mats as dispersers of fish species in the region.Nesse trabalho as assembleias de peixes associadas a bancos de macrófitas flutuantes à deriva foram descritas. Além disso, foi considerado o possível papel desses bancos como vetores de dispersão no rio Ivinhema, importante tributário do alto rio Paraná, Brasil. Os peixes associados aos bancos à deriva foram amostrados no canal principal desse rio, entre os meses de Janeiro a Março de 2005, quando o vento e/ou o aumento no nível da água foram suficientes para transportar os bancos de macrófitas. Os peixes foram amostrados com uma rede flutuante (4 m de comprimento x 2 m de largura x 0,6 m de altura e 2 mm de tamanho de malha. No

  16. A MODIS-Based Novel Method to Distinguish Surface Cyanobacterial Scums and Aquatic Macrophytes in Lake Taihu

    National Research Council Canada - National Science Library

    Liang, Qichun; Zhang, Yuchao; Ma, Ronghua; Loiselle, Steven; Li, Jing; Hu, Minqi

    2017-01-01

      Satellite remote sensing can be an effective alternative for mapping cyanobacterial scums and aquatic macrophyte distribution over large areas compared with traditional ship's site-specific samplings...

  17. Characteristics and degradation of carbon and phosphorus from aquatic macrophytes in lakes: Insights from solid-state 13C NMR and solution 31P NMR spectroscopy

    Science.gov (United States)

    LIU, S. S.; Zhu, Y.; Meng, W.; Wu, F.

    2016-12-01

    Water extractable organic matter (WEOM) derived from macrophytes plays an important role in biogeochemical cycling of nutrients, including carbon (C), nitrogen (N) and phosphorus (P) in lakes. However, reports of their composition and degradation in natural waters are scarce. Therefore, compositions and degradation of WEOM derived from six aquatic macrophytes species of Tai Lake, China, were investigated by use of solid-state 13C NMR and solution 31P NMR spectroscopy. Carbohydrates were the predominant constituents of WEOM fractions, followed by carboxylic acid. Orthophosphate (ortho-P) was the dominant form of P (78.7% of total dissolved P) in the water extracts, followed by monoester P (mono-P) (20.6%) and little diester P (0.65%). The proportion of mono-P in total P species increased with the percentage of O-alkyl and O-C-O increasing in the WEOM, which is likely due to degradation and dissolution of biological membranes and RNA from aquatic plants. Whereas the proportion of mono-P decreased with alkyl-C, NCH/OCH3 and COO/N-C=O increasing, which may be owing to the insoluble compounds including C functional groups of alkyl-C, NCH/OCH3 and COO/N-C=O, such as aliphatic biopolymers, lignin and peptides. Based on the results of this study and information in the literature about water column and sediment, we propose that WEOM, dominated by polysaccharides, are the most labile and bioavailable component in debris of macrophytes. Additionally, these WEOMs would also be a potential source for bioavailable organic P (e.g., RNA, DNA and phytate) for lakes.

  18. Spinach seed quality - potential for combining seed size grading and chlorophyll flourescence sorting

    DEFF Research Database (Denmark)

    Deleuran, Lise Christina; Olesen, Merete Halkjær; Boelt, Birte

    2013-01-01

    might therefore improve the establishment of spinach for producers. Spinach seeds were harvested at five different times (H1, H2, H3, H4 and H5) starting 3 weeks before estimated optimum harvest time. The harvested seeds were sorted according to chlorophyll fluorescence (CF) and seed size. Two harvest.......5–3.25 mm size seeds had germinated on day 3 than both their larger and smaller counterparts at the later time of harvest (H4). Seeds with a diameter below 2.5 mm displayed the lowest MGT. Commercially, the use of chlorophyll fluorescence (CF)-sorted seeds, in combination with seed size sorting, may provide...

  19. Effect of Ozone Treatment on Inactivation of Escherichia coli and Listeria sp. on Spinach

    OpenAIRE

    Shreya Wani; Jagpreet K. Maker; Joseph R. Thompson; Jeremy Barnes; Ian Singleton

    2015-01-01

    The efficacy of “gaseous” ozone in reducing numbers and re-growth of food-borne pathogens, (Escherichia coli and Listeria spp.), on leafy salads was investigated using spinach. A preliminary in vivo study showed 1-log reduction in six strains of E. coli and two species of Listeria spp. on spinach exposed to 1 ppm ozone for 10 min. A range of ozone treatments were explored to deliver optimal bacterial inactivation while maintaining the visual appearance (color) of produce. Exposure to a highe...

  20. In vitro liberation of carotenoids from spinach and Asia Salads after different domestic kitchen procedures

    DEFF Research Database (Denmark)

    Eriksen, Jane Nygaard; Luu, Amy Y; Dragsted, Lars Ove

    2016-01-01

    Green-leafy vegetables are rich in nutritionally important constituents including carotenoids. Their potential health benefits depend among others on their liberation from the plant matrix. The aim of the present study was to evaluate the effect of particle size and heat treatments on lutein and β...... was shown to be nullified by the severe heat impact during stir-frying of minced spinach, showing that domestic treatments need to be chosen carefully to maximise carotenoid liberation. Steaming significantly improved lutein liberation from Asia salads, but had no or a negative effect in spinach samples...

  1. Flavonoids in baby spinach (Spinacia oleracea L.): Changes during plant growth and storage

    DEFF Research Database (Denmark)

    Bergquist, S. Å. M.; Gertsson, U. E.; Knuthsen, Pia

    2005-01-01

    The variation in flavonoid concentration and composition was investigated in baby spinach (Spinacia oleracea L.) cv. Emilia sown on three occasions, each harvested at three growth stages at 6-day intervals. After harvest, leaves were stored in polypropylene bags at 2 or 10 degrees C. Flavonoids......-12% of the total flavonoid content. Total flavonoid content was relatively stable during normal retail storage conditions, although some of the individual flavonoid compounds showed considerable variation. The youngest plants had the highest flavonoid concentration, indicating that by harvesting the baby spinach...

  2. Ability of four emergent macrophytes to remediate permethrin in mesocosm experiments.

    Science.gov (United States)

    Moore, M T; Kröger, R; Cooper, C M; Smith, S

    2009-08-01

    Increased focus is being placed on the ability of native vegetation to mitigate potential harmful effects of agricultural runoff, especially pyrethroid insecticides. Replicate 379 L Rubbermaid tubs (1.25 m [l] x 0.6 m [w] x 0.8 m [h]) were planted with individual species of cutgrass (Leersia oryzoides), cattails (Typha latifolia), bur-reed (Sparganium americanum), and powdery alligator-flag (Thalia dealbata), all common wetland macrophytes found in the Mississippi Delta, USA, agricultural region. Permethrin-enriched water (target concentration, 5 microg L(-1)) was pumped in at a 4-h hydraulic retention time at one end of the tub and discharged at the far end. Water samples were collected from discharge at 1-h intervals for 12 h and analyzed for permethrin concentrations. Permethrin removal rates were compared for the four different plant treatments and nonvegetated sediment-water controls. Results indicated that no particular single plant species was more effective at removing permethrin in water relative to unplanted controls. Overall mass reductions (from inflow to outflow) for cis-permethrin ranged from 67% +/- 6% in T. latifolia to 71% +/- 2% in L. oryzoides. The trans-permethrin overall mass reductions ranged from 76% +/- 4% in S. americanum to 82% +/- 2% in the unplanted control. Sediment and plant samples collected at the study conclusion indicated that 77%-95% of measured permethrin mass was associated with sediment for mesocosms planted with L. oryzoides, T. latifolia, and T. dealbata. Conversely, mesocosms planted with S. americanum had 83% of measured mass associated with the plant material. Specific plant-pesticide retention studies can lead to improved planning for best management practices and remediation techniques such as constructed wetlands and vegetated agricultural drainage ditches.

  3. Monitoring the effects of floods on submerged macrophytes in a large river.

    Science.gov (United States)

    Ibáñez, Carles; Caiola, Nuno; Rovira, Albert; Real, Montserrat

    2012-12-01

    The lower Ebro River (Catalonia, Spain) has recently undergone a regime shift from a phytoplankton to a macrophyte-dominated system. Macrophytes started to spread at the end of the 1990s and since 2002 artificial floods (flushing flows) of short duration (1-2 days) are released from the Riba-roja dam once or twice a year in order to reduce macrophyte density. The aim of this study was to analyse the spatiotemporal trends of the submerged macrophytes in two stretches of the lower Ebro River using high-resolution hydroacoustic methods, in order to elucidate the effects of artificial floods and natural floods on its distribution and abundance. Results showed that the mean cover in the two studied stretches (Móra and Ginestar) was not reduced after a flushing flow (from 36.59% to 55.85% in Móra, and from 21.18% to 21.05% in Ginestar), but it was greatly reduced after the natural flood (down to 9.79% in Móra and 2.04% in Ginestar); surprisingly the cover increased in Móra after the artificial flood. In order to increase the efficiency of floods in controlling macrophyte spreading, the magnitude and frequency of them should largely increase, as well as the suspended sediment load, approaching as much as possible to the original flood pattern before dam construction. Hydroacoustic methods combined with geostatistics and interpolation in GIS can accurately monitor spatiotemporal trends of submerged macrophytes in large rivers. This is the first article to apply this monitoring system to submerged macrophytes in rivers. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Farm management, environment, and weather factors jointly affect the probability of spinach contamination by generic Escherichia coli at the preharvest stage.

    Science.gov (United States)

    Park, Sangshin; Navratil, Sarah; Gregory, Ashley; Bauer, Arin; Srinath, Indumathi; Szonyi, Barbara; Nightingale, Kendra; Anciso, Juan; Jun, Mikyoung; Han, Daikwon; Lawhon, Sara; Ivanek, Renata

    2014-04-01

    The National Resources Information (NRI) databases provide underutilized information on the local farm conditions that may predict microbial contamination of leafy greens at preharvest. Our objective was to identify NRI weather and landscape factors affecting spinach contamination with generic Escherichia coli individually and jointly with farm management and environmental factors. For each of the 955 georeferenced spinach samples (including 63 positive samples) collected between 2010 and 2012 on 12 farms in Colorado and Texas, we extracted variables describing the local weather (ambient temperature, precipitation, and wind speed) and landscape (soil characteristics and proximity to roads and water bodies) from NRI databases. Variables describing farm management and environment were obtained from a survey of the enrolled farms. The variables were evaluated using a mixed-effect logistic regression model with random effects for farm and date. The model identified precipitation as a single NRI predictor of spinach contamination with generic E. coli, indicating that the contamination probability increases with an increasing mean amount of rain (mm) in the past 29 days (odds ratio [OR] = 3.5). The model also identified the farm's hygiene practices as a protective factor (OR = 0.06) and manure application (OR = 52.2) and state (OR = 108.1) as risk factors. In cross-validation, the model showed a solid predictive performance, with an area under the receiver operating characteristic (ROC) curve of 81%. Overall, the findings highlighted the utility of NRI precipitation data in predicting contamination and demonstrated that farm management, environment, and weather factors should be considered jointly in development of good agricultural practices and measures to reduce produce contamination.

  5. The effect of foliar nutrition of spinach (Spinacia oleracea L. with magnesium salts and urea on gas exchange, leaf yield and quality

    Directory of Open Access Journals (Sweden)

    Edward Borowski

    2012-12-01

    Full Text Available In a pot experiment conducted in a phytotron, the effectiveness of foliar nutrition of spinach (Spinacia oleracea L. with different magnesium salts with and without the addition of 0.5% CO(NH22 was studied. Magnesium was applied 3 times in the form of solutions of MgSO4 x 7H2O, Mg(NO32 x 6H2O, MgCl2 x 6H2O, C4H6O4Mg x 4H2O, compared to water as the control treatment. The obtained results showed that foliar feeding of spinach with inorganic magnesium salts was an efficient method for supplementing the Mg level in plants during the growing period. But the application of a metalo-organic complex in the form of magnesium acetate (C4H6O4Mg x 4H2O at a concentration of 1.7%, in spite of a similar effect on leaf Mg content, induced phytotoxic symptoms in the form of chlorotic and necrotic spots on the leaves. The application of the solutions of inorganic magnesium salts had a significant effect, resulting in more intensive leaf gas exchange (stomatal conductance, transpiration and photosynthesis and an increase in leaf yield. Magnesium sulphate affected the abovementioned processes in the most effective way, while magnesium acetate had a negative impact. Foliar feeding of spinach with the magnesium salts resulted in an increased leaf content of protein, chlorophyll, carotenoids, nitrates and proline, but a decrease in vitamin C content. The addition of urea to the applied magnesium salt solutions increased the plant gas exchange rates and the leaf content of protein, chlorophyll, carotenoids, nitrates and proline, but it decreased the content of vitamin C, potassium and magnesium.

  6. Occurrence and genetic diversity of Arcobacter spp. in a spinach-processing plant and evaluation of two Arcobacter-specific quantitative PCR assays.

    Science.gov (United States)

    Hausdorf, Lena; Neumann, Maria; Bergmann, Ingo; Sobiella, Kerstin; Mundt, Kerstin; Fröhling, Antje; Schlüter, Oliver; Klocke, Michael

    2013-06-01

    Some species of the genus Arcobacter are considered to be emerging food pathogens. With respect to recent vegetable-borne outbreaks, the aim of this work was to investigate the occurrence and diversity of Arcobacter within the production chain of a spinach-processing plant by a combination of cultivation and molecular methods. Samples including spinach, water, and surface biofilm were taken over a period of three years from the entire processing line. Ten 16S rRNA (rrs) gene clone libraries were constructed and analysed using amplified rRNA gene restriction analysis (ARDRA). Approximately 1200 clones were studied that resulted in 44 operational taxonomic units (OTUs). Sequences with high similarities to Arcobacter cryaerophilus (13% of clones, 3 OTUs), A. ellisii (4%, 6 OTUs), A. suis (15%, 3 OTUs), and the type strain of A. nitrofigilis (1%, 7 OTUs) were identified. This represents the first report of the detection of the recently described species A. ellisii, A. suis and, in addition, A. venerupis from alternative habitats. A total of 67% of the clones (22 OTUs) could not be assigned to a genus, which indicated the presence of uncharacterised Arcobacter species. For the cultivation-independent detection of Arcobacter, two genus-specific quantitative PCR (qPCR) assays were developed and tested on 15 Arcobacter species. When these assays were applied to samples from the spinach-processing plant, they showed positive results for up to 35% of the samples and supported the conclusion that there is a considerable risk for the transfer of pathogenic Arcobacter species on vegetables, which was also verified by a cultivation approach.

  7. Evaluation of butachlor for control of some submerged macrophytes along with its impact on biotic components of freshwater system

    Directory of Open Access Journals (Sweden)

    A. Chattopadhyay, S. Adhikari, S. P. Adhikary, S. Ayyappan

    2006-04-01

    Full Text Available In this investigation, the efficacy of the herbicide butachlor, (N-butoxymethyl-2 chloro-21, 61 diethyl acetanilide was tested against few common submerged macrophytes namely Hydrilla (Hydrilla verticillata (L. Royale, Najas (Najas minor All., Nechamandra (Nechamandra alternifolia (Roxb. Thwaites and Ottelia (Ottelia alismoides (L. Pers. of freshwater fish ponds. Almost complete decay of Hydrilla, Nechamandra and Ottelia was achieved at 7.5 L of active ingredient/ha/m butachlor within 15 days while the herbicide showed no negative effect on Najas. However at the same concentration of butachlor, total mortality of zooplankton and water fern Azolla (Azolla caroliniana Lamarck occurred within seven days. In case of few freshwater fish species like Rohu (Labeo rohita, Channa (Channa punctatus, Anabas (Anabas testitudineus and Heteropneustes (Heteropneustes fossilis, total mortality occurred upto 90 days after application of the same dose of butachlor but fish survived beyond 120 days of herbicide application indicating degradation of the herbicides.

  8. Effects of High Temperature Frying of Spinach Leaves in Sunflower Oil on Carotenoids, Chlorophylls, and Tocopherol Composition

    Science.gov (United States)

    Zeb, Alam; Nisar, Parveen

    2017-01-01

    Spinach is one of the highly consumed vegetable, with significant nutritional, and beneficial properties. This study revealed for the first time, the effects of high temperature frying on the carotenoids, chlorophylls, and tocopherol contents of spinach leaves. Spinach leaves were thermally processed in the sunflower oil for 15, 30, 45, and 60 min at 250°C. Reversed phase HPLC-DAD results revealed a total of eight carotenoids, four chlorophylls and α-tocopherol in the spinach leaves. Lutein, neoxanthin, violaxanthin, and β-carotene-5,6-epoxide were the major carotenoids, while chlorophyll a and b' were present in higher amounts. Frying of spinach leaves increased significantly the amount of α-tocopherol, β-carotene-5,6-epoxide, luteoxanthin, lutein, and its Z-isomers and chlorophyll b' isomer. There was significant decrease in the amounts of neoxanthin, violaxanthin, chlorophyll b, b' and chlorophyll a with increase of frying time. The increase of frying time increased the total phenolic contents in spinach leaves and fried sunflower oil samples. Chemical characteristics such as peroxide values, free fatty acids, conjugated dienes, conjugated trienes, and radical scavenging activity were significantly affected by frying, while spinach leaves increased the stability of the frying oil. This study can be used to improve the quality of fried vegetable leaves or their products at high temperature frying in food industries for increasing consumer acceptability. PMID:28382299

  9. Effects of High Temperature Frying of Spinach Leaves in Sunflower Oil on Carotenoids, Chlorophylls, and Tocopherol Composition.

    Science.gov (United States)

    Zeb, Alam; Nisar, Parveen

    2017-01-01

    Spinach is one of the highly consumed vegetable, with significant nutritional, and beneficial properties. This study revealed for the first time, the effects of high temperature frying on the carotenoids, chlorophylls, and tocopherol contents of spinach leaves. Spinach leaves were thermally processed in the sunflower oil for 15, 30, 45, and 60 min at 250°C. Reversed phase HPLC-DAD results revealed a total of eight carotenoids, four chlorophylls and α-tocopherol in the spinach leaves. Lutein, neoxanthin, violaxanthin, and β-carotene-5,6-epoxide were the major carotenoids, while chlorophyll a and b' were present in higher amounts. Frying of spinach leaves increased significantly the amount of α-tocopherol, β-carotene-5,6-epoxide, luteoxanthin, lutein, and its Z-isomers and chlorophyll b' isomer. There was significant decrease in the amounts of neoxanthin, violaxanthin, chlorophyll b, b' and chlorophyll a with increase of frying time. The increase of frying time increased the total phenolic contents in spinach leaves and fried sunflower oil samples. Chemical characteristics such as peroxide values, free fatty acids, conjugated dienes, conjugated trienes, and radical scavenging activity were significantly affected by frying, while spinach leaves increased the stability of the frying oil. This study can be used to improve the quality of fried vegetable leaves or their products at high temperature frying in food industries for increasing consumer acceptability.

  10. Comparative Study of Betacyanin Profile and Antimicrobial Activity of Red Pitahaya (Hylocereus polyrhizus) and Red Spinach (Amaranthus dubius).

    Science.gov (United States)

    Yong, Yi Yi; Dykes, Gary; Lee, Sui Mae; Choo, Wee Sim

    2017-03-01

    Betacyanins are reddish to violet pigments that can be found in red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius). This study investigated the impact of sub-fractionation (solvent partitioning) on betacyanin content in both plants. Characterization of betacyanins and evaluation of their antimicrobial activities were also carried out. Betanin was found in both plants. In addition, isobetanin, phyllocactin and hylocerenin were found in red pitahaya whereas amaranthine and decarboxy-amaranthine were found in red spinach. Sub-fractionated red pitahaya and red spinach had 23.5 and 121.5 % more betacyanin content, respectively, than those without sub-fractionation. Sub-fractionation increased the betanin and decarboxy-amaranthine content in red pitahaya and red spinach, respectively. The betacyanin fraction from red spinach (minimum inhibitory concentration [MIC] values: 0.78-3.13 mg/mL) demonstrated a better antimicrobial activity profile than that of red pitahaya (MIC values: 3.13-6.25 mg/mL) against nine Gram-positive bacterial strains. Similarly, the red spinach fraction (MIC values: 1.56-3.13 mg/mL) was more active than the red pitahaya fraction (MIC values: 3.13-6.25 mg/mL) against five Gram-negative bacterial strains. This could be because of a higher amount of betacyanin, particularly amaranthine in the red spinach.

  11. Spectrophotometric studies on alkaline isomerization of spinach ferredoxin.

    Science.gov (United States)

    Hasumi, H; Nagata, E; Nakamura, S

    1985-10-01

    The gross protein structure, the microenvironment of the iron-sulfur cluster, and the effect of neutral salts on the molecular structure of spinach ferredoxin were studied by CD and absorption spectroscopy in the alkaline pH range. In the pH range of 7-11, the existence of reversible isomerization which consisted of at least two proton dissociation processes was indicated by the statical CD and absorption spectra. The CD changes in the visible and far-UV regions were dramatic upon elevation of the pH from neutral to alkaline, indicating a significant alteration of the microenvironment of the cluster and a decrease in the ordered secondary structures. The absorption change in the visible region due to pH elevation was small but clearly observed with a high signal-to-noise ratio. The numbers of protons involved in the respective processes and the apparent pK values obtained from the pH-dependence of the CD changes were in good agreement with those obtained from the pH-dependence of the absorption changes in the visible region. In addition, the rate constants obtained from the time courses of the CD and absorption changes agreed with one another. By the addition of 1 M NaCl, the CD and absorption spectra at alkaline pH were reversed almost to those at neutral pH without significant pH change. On the other hand, above pH 11, ferredoxin was found to be irreversibly denatured. Based on analyses of the statical CD and absorption spectra and of the time courses of the CD changes, the probable mechanism of the isomerization was considered to be as follows: (Formula: see text) where H stands for a proton, N-form for native ferredoxin at neutral pH, N*-form for alkaline ferredoxin below pH 11 which still has the iron-sulfur cluster but with disordered secondary structures of the polypeptide chain, and D-form for completely denatured ferredoxin above pH 11. These results lead to the conclusions that (1) the interaction between the protein moiety and the iron-sulfur cluster is

  12. Preventing an increase in Verticillium wilt incidence in spinach seed production

    DEFF Research Database (Denmark)

    Olesen, Merete Halkjær; Deleuran, Lise Christina; Gislum, René;

    2014-01-01

    A semifield assay was conducted from 2009 to 2011 to distinguish between different preventive methods of reducing Verticillium spp. in spinach seed production. The seed treatments for controlling seed infection levels included Thiram, Signum, Trichoderma harzianum, Gliocladium roseum and Natural II...

  13. Internalization of E. coli O157:H7 in spinach cultivated in soil and hydroponic media

    Science.gov (United States)

    Introduction: Internalization of E. coli O157:H7 into spinach plants through root uptake is a potential route of contamination. Previous studies that have investigated uptake of E. coli O157:H7 into leafy greens have expressed green fluorescent protein (gfp) from a plasmid, possibly limiting detecti...

  14. Phenotypic changes in different spinach varieties grown and selected under organic conditions

    NARCIS (Netherlands)

    Serpolay, E.; Schermann, N.; Dawson, J.C.; Lammerts Van Bueren, E.; Goldringer, I.; Chable, V.

    2011-01-01

    Organic and low-input agriculture needs flexible varieties that can buffer environmental stress and adapt to the needs of farmers. We implemented an experiment to investigate the evolutionary capacities of a sample of spinach (Spinacia oleracea L.) population varieties for a number of phenotypic tra

  15. Folate Biofortification in Hydroponically Cultivated Spinach by the Addition of Phenylalanine.

    Science.gov (United States)

    Watanabe, Sho; Ohtani, Yuta; Tatsukami, Yohei; Aoki, Wataru; Amemiya, Takashi; Sukekiyo, Yasunori; Kubokawa, Seiichi; Ueda, Mitsuyoshi

    2017-06-14

    Folate is an important vitamin mainly ingested from vegetables, and folate deficiency causes various health problems. Recently, several studies demonstrated folate biofortification in plants or food crops by metabolic engineering through genetic modifications. However, the production and sales of genetically modified foods are under strict regulation. Here, we developed a new approach to achieve folate biofortification in spinach (Spinacia oleracea) without genetic modification. We hydroponically cultivated spinach with the addition of three candidate compounds expected to fortify folate. As a result of liquid chromatography tandem mass spectrometry analysis, we found that the addition of phenylalanine increased the folate content up to 2.0-fold (306 μg in 100 g of fresh spinach), representing 76.5% of the recommended daily allowance for adults. By measuring the intermediates of folate biosynthesis, we revealed that phenylalanine activated folate biosynthesis in spinach by increasing the levels of pteridine and p-aminobenzoic acid. Our approach is a promising and practical approach to cultivate nutrient-enriched vegetables.

  16. Spinach and mustard greens response to soil type, sulfur addition and lithium level

    Science.gov (United States)

    A greenhouse experiment was conducted near Weslaco, Texas (Lat. 26o 8' N, Long. 97o 57' W) between Dec. 2006 and Feb 2007 to evaluate the effect of soil type, added sulfur and lithium level on the growth and leaf nutrients, particularly biofortified levels of Li and S, in spinach and mustard gree...

  17. Genetic and nutritional characterization of some macrophytes, inhabiting the Bardawil Lagoon, Sinai, Egypt

    Directory of Open Access Journals (Sweden)

    Hosam E. Elsaied

    2015-12-01

    Full Text Available The ecological and economical significances of macrophytes, inhabiting the Mediterranean Lagoon, Bardawil, northern Sinai, Egypt, are still ambiguous, due to lack of knowledge. This study focused on genetic and nutritional characterization of three dominant macrophyte species at Bardawil Lagoon. Genetic identifications were done through genomic DNA extraction, followed by PCR amplifications and sequencing of 18S rRNA genes of the studied species. Phylogenetic analyses indicated that two of the recorded species showed homologies with the seagrass species, Posidonia oceanica and Halophila ovalis, with nucleotide identities 94.5% and 96.8%, respectively. The third species showed a unique phylogenetic lineage, representing nucleotide identity average, 86.5%, among the brown seaweeds, Heterokontophyta. Nutritional analyses indicated that the recorded seaweed-like macrophyte had the highest recommended nutritional contents, crude protein, 24.67%, with a total amino acid composition of 6.64 g/100 g protein, and carbohydrate, 38.16%, besides a calorific value of 3.063 K cal/g, among the studied macrophytes. To the best of our knowledge, this is the first attempt to characterize macrophyte community in Bardawil Lagoon, using both genetic and biochemical approaches.

  18. How TK-TD and population models for aquatic macrophytes could support the risk assessment for plant protection products

    NARCIS (Netherlands)

    Hommen, U.; Schmitt, W.; Heine, S.; Brock, T.C.M.; Duquesne, S.; Manson, P.; Meregali, G.; Ochoa-Acuna, H.; Vliet, van P.; Arts, G.H.P.

    2016-01-01

    This case study of the SETAC workshop MODELINK demonstrates the potential use of mechanistic effects models for macrophytes to extrapolate from effects of a plant protection product observed in laboratory tests to effects resulting from dynamic exposure on macrophyte populations in edge-of-field wat

  19. Bimodality in stable isotope composition facilitates the tracing of carbon transfer from macrophytes to higher trophic levels

    NARCIS (Netherlands)

    Mendonca, R.; Kosten, S.; Lacerot, G.; Mazzeo, N.; Roland, F.; Ometto, J.P.; Paz, A.; Bueno, O.C.; Gomes, A.C.M.M.; Scheffer, M.

    2013-01-01

    Even though the suitability of macrophytes to act as a carbon source to food webs has been questioned by some studies, some others indicate that macrophyte-derived carbon may play an important role in the trophic transfer of organic matter in the food web of shallow lakes. To evaluate the importance

  20. Mercury contents in aquatic macrophytes from two reservoirs in the Paraíba do Sul: Guandú river system, SE Brazil.

    Science.gov (United States)

    Molisani, M M; Rocha, R; Machado, W; Barreto, R C; Lacerda, L D

    2006-02-01

    This paper reports on a study to determine the Hg content in the five most abundant aquatic macrophyte species (Elodea densa, Sagittaria montevidensis, Salvinia auriculata, Pistia stratiotes and Eichhornia crassipes) in two artificial reservoirs flooded by water diverted from the Paraíba do Sul river, SE Brazil. The potential of these species for Hg accumulation and their role in Hg transport along the river system due to macrophyte management were evaluated. Mercury concentrations were higher in free-floating than in rooted species. Roots were also richer in Hg than were leaves. Dry weight Hg concentrations in leaves and roots from all species varied from 46-246 ng.g(-1) to 37-314 ng.g(-1), respectively. These values are higher than those reported for uncontaminated lakes in Brazil and in other tropical areas and similar to those reported for moderately contaminated sites. Mercury concentrations can be attributed to fluvial transport from the heavily industrialized Paraíba do Sul river basin. Intensive sampling of Pistia stratiotes from two sites in the Vigário reservoir was performed to evaluate the capacity of Hg incorporation in short periods of time. The results showed a significant negative correlation between Hg content and size class of individual plants, demonstrating the importance of juveniles, fast growing plants in absorbing Hg. The foremost impact related to Hg contents in the studied area concerns the periodic removal of macrophytes for reservoir management, followed by disposal in nearby areas. This results in the mobilization of 0.52 to 1.3 Kg of Hg per year, a significant fraction of the Hg burden present in reservoir waters. Disposal of such material may result in Hg leaching to river systems, affecting the Hg transfer throughout the basin.

  1. Mercury contents in aquatic macrophytes from two reservoirs in the Paraíba do Sul: Guandú river system, SE Brazil

    Directory of Open Access Journals (Sweden)

    M. M. Molisani

    Full Text Available This paper reports on a study to determine the Hg content in the five most abundant aquatic macrophyte species (Elodea densa, Sagittaria montevidensis, Salvinia auriculata, Pistia stratiotes and Eichhornia crassipes in two artificial reservoirs flooded by water diverted from the Paraíba do Sul river, SE Brazil. The potential of these species for Hg accumulation and their role in Hg transport along the river system due to macrophyte management were evaluated. Mercury concentrations were higher in free-floating than in rooted species. Roots were also richer in Hg than were leaves. Dry weight Hg concentrations in leaves and roots from all species varied from 46-246 ng.g-1 to 37-314 ng.g-1, respectively. These values are higher than those reported for uncontaminated lakes in Brazil and in other tropical areas and similar to those reported for moderately contaminated sites. Mercury concentrations can be attributed to fluvial transport from the heavily industrialized Paraíba do Sul river basin. Intensive sampling of Pistia stratiotes from two sites in the Vigário reservoir was performed to evaluate the capacity of Hg incorporation in short periods of time. The results showed a significant negative correlation between Hg content and size class of individual plants, demonstrating the importance of juveniles, fast growing plants in absorbing Hg. The foremost impact related to Hg contents in the studied area concerns the periodic removal of macrophytes for reservoir management, followed by disposal in nearby areas. This results in the mobilization of 0.52 to 1.3 Kg of Hg per year, a significant fraction of the Hg burden present in reservoir waters. Disposal of such material may result in Hg leaching to river systems, affecting the Hg transfer throughout the basin.

  2. Ammonia-oxidizing Bacteria and Archaea in the Rhizosphere of Freshwater Macrophytes

    DEFF Research Database (Denmark)

    Herrmann, Martina; Schramm, Andreas

    2007-01-01

    measurements revealed clear differences in ammonia oxidation rates. The diversity of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) was assessed using the ammonia monooxygenase (amoA) gene as functional marker. Both AOA and AOB could be detected in the rhizosphere of all three plant......AMMONIA-OXIDIZING ARCHAEA AND BACTERIA IN THE RHIZOSPHERE OF FRESHWATER MACROPHYTES Martina Herrmann and Andreas Schramm Department of Biological Sciences, Microbiology, University of Aarhus, Denmark Aquatic macrophytes such as Littorella uniflora and Lobelia dortmanna release oxygen from......-specific microbial community distinct from that of unvegetated sediment and (ii) that aquatic macrophytes have an impact on abundance and activity of nitrifying and denitrifying bacteria in freshwater sediment. The goal of this study was to test these hypotheses for the key functional group for coupled nitrification...

  3. Effects of high nitrogen concentrations on the growth of submersed macrophytes at moderate phosphorus concentrations.

    Science.gov (United States)

    Yu, Qing; Wang, Hong-Zhu; Li, Yan; Shao, Jian-Chun; Liang, Xiao-Min; Jeppesen, Erik; Wang, Hai-Jun

    2015-10-15

    Eutrophication of lakes leading to loss of submersed macrophytes and higher turbidity is a worldwide phenomenon, attributed to excessive loading of phosphorus (P). However, recently, the role of nitrogen (N) for macrophyte recession has received increasing attention. Due to the close relationship between N and P loading, disentanglement of the specific effects of these two nutrients is often difficult, and some controversy still exists as to the effects of N. We studied the effects of N on submersed macrophytes represented by Vallisneria natans (Lour.) Hara in pots positioned at three depths (0.4 m, 0.8 m, and 1.2 m to form a gradient of underwater light conditions) in 10 large ponds having moderate concentrations of P (TP 0.03 ± 0.04 mg L(-1)) and five targeted concentrations of total nitrogen (TN) (0.5, 2, 10, 20, and 100 mg L(-1)), there were two ponds for each treatment. To study the potential shading effects of other primary producers, we also measured the biomass of phytoplankton (ChlaPhyt) and periphyton (ChlaPeri) expressed as chlorophyll a. We found that leaf length, leaf mass, and root length of macrophytes declined with increasing concentrations of TN and ammonium, while shoot number and root mass did not. All the measured growth indices of macrophytes declined significantly with ChlaPhyt, while none were significantly related to ChlaPeri. Neither ChlaPhyt nor ChlaPeri were, however, significantly negatively related to the various N concentrations. Our results indicate that shading by phytoplankton unrelated to the variation in N loading and perhaps toxic stress exerted by high nitrogen were responsible for the decline in macrophyte growth.

  4. Advances in ecological function and interaction mechanism of aquatic macrophyte-biofilm system%水生植物-生物膜体系的生态功能与互作机制研究进展

    Institute of Scientific and Technical Information of China (English)

    吕小央; 张松贺; 刘凯辉; 韩冰; 郭川

    2015-01-01

    水生植物是水生态系统的重要组成部分,是生物膜的天然载体,水生植被的恢复有利于提高水体生物膜面积。与非生物介质相比,水生植物与微生物间存在附着互作机制,一方面水生植物能为微生物提供附着场所和营养物质,其产生的化感物质对附着微生物的种类和数量有影响;另一方面,附着微生物也会对水生植物生长产生促进或遏制作用。另外,指出水流和水质对水生植物及生物膜的形成、结构以及群落组成产生重要影响;水生植物和生物膜作为一个复杂互作体系,是水体生态系统的基本单位,相关植物表面表面生物膜体系特征及其对水体中碳、氮等要素的转化及污染物的去除机制相关研究,将是今后水生态研究过程中的重点方向之一。%Being the key component of aquatic ecosystem , the aquatic macrophyte is a natural carrier of biofilm . The restoration of aquatic macrophyte contributes to the increase of water area of biofilm .Compared with the non-biological medium , there were a complex interaction mechanism between the aquatic macrophyte and the microbe . On one hand , aquatic macrophyte provide the attachment sites and nutrients for the microbe , and the allelochemicals emerged from the aquatic macrophyte have an effect on the types and quantity of attached microbe ;on the other hand , the attached microbe can promote or restrain the growth of aquatic macrophyte .Additionally , flow and water quality can have important effects on the formation , structure and the composition of community of aquatic macrophyte and biofilm . As a complex interaction system , aquatic macrophyte and biofilm is the fundamental unit of water ecosystem .The study on the characteristics of plant surface-surface biofilm system and its role on the transformation of carbon , nitrogen and other elements in water , and the pollutants removal mechanism should be one of the

  5. Determination of low levels of perchlorate in lettuce and spinach using ion chromatography-electrospray ionization mass spectrometry (IC-ESI-MS).

    Science.gov (United States)

    Seyfferth, Angelia L; Parker, David R

    2006-03-22

    A sample preparation method was developed to quantify environmentally relevant (low micrograms per liter) concentrations of perchlorate (ClO4(-)) in leafy vegetables using IC-ESI-MS. Lettuce and spinach were macerated, centrifuged, and filtered, and the aqueous extracts were rendered water-clear using a one-step solid-phase extraction method. Total time for extraction and sample preparation was 6 h. Ion suppression was demonstrated and was likely due to unknown organics still present in the extract solution after cleanup. However, this interference was readily eliminated using a Cl(18)O4(-) internal standard at 1 microg/L in all standards and samples. Hydroponically grown perchlorate-free butterhead lettuce was spiked to either 10.3 or 37.7 microg/kg of fresh weight (FW), and recoveries were between 91 and 98% and between 93 and 101%, respectively. Five types of lettuce and spinach from a local grocery store were then analyzed; they contained from 0.6 to 6.4 microg/kg of FW. Spike recoveries using the store-bought samples ranged from 89 to 100%. The method detection limit for perchlorate in plant extracts is 40 ng/L, and the corresponding minimum reporting limit is 200 ng/L or 0.8 microg/kg of FW.

  6. Ammonia-oxidizing Bacteria and Archaea in the Rhizosphere of Freshwater Macrophytes

    DEFF Research Database (Denmark)

    Herrmann, Martina; Schramm, Andreas

    2007-01-01

    AMMONIA-OXIDIZING ARCHAEA AND BACTERIA IN THE RHIZOSPHERE OF FRESHWATER MACROPHYTES Martina Herrmann and Andreas Schramm Department of Biological Sciences, Microbiology, University of Aarhus, Denmark Aquatic macrophytes such as Littorella uniflora and Lobelia dortmanna release oxygen from...... measurements revealed clear differences in ammonia oxidation rates. The diversity of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) was assessed using the ammonia monooxygenase (amoA) gene as functional marker. Both AOA and AOB could be detected in the rhizosphere of all three plant...

  7. The effects of different fish species on growth of submerged macrophytes%不同鱼类对沉水植物生长的影响∗

    Institute of Scientific and Technical Information of China (English)

    王晓平; 王玉兵; 杨桂军; 秦伯强; 杨宏伟

    2016-01-01

    Submerged macrophyte is an important component in ecological restoration of eutrophic waters. Fish is one of the impor⁃tant factors affecting the growth of submerged macrophytes. In the present study, the effects of three fish species( Ctenopharyngodon idellus, Parabramis pekinensis and Carassius auratus) on growth of four submerged macrophytes( Vallisneria natans, Ceratophyllum demersum, Elodea nuttalli and Potamogeton malaianus) were studied. Results showed that the decline rates of submerged macro⁃phytes biomass were 19.2%, 82.1% and 16.6% in controls, C. idellus and P. pekinensis, respectively. However, submerged mac⁃rophytes biomass increased 12.5% in the C. auratus treatments. The rate of increase of Ceratophyllum demersum biomss was the highest among the four submerged macrophytes. The rate of increase of C. demersum biomass in controls, P. pekinensis and C. aura⁃tus treatments were 171.1%,113.9% and 265.5%, respectively, but that was -24.4% in C. idellus treatments. Moreover, all the three fish species inhibited the growth of Spirogyra which is one of the competitors of submerged macrophytes. The results of this study could be used for the fish management during the restoration of submerged macrophytes in eutrophic waters.%沉水植物是富营养水体生态修复的重要组成部分,鱼类是影响沉水植物生长的重要因素之一.为了解鱼类对沉水植物生长的影响,研究3种鱼(草鱼( Ctenopharyngodon idellus)、鲫( Carassius auratus)、鳊( Parabramis pekinensis))对4种沉水植物(苦草( Vallisneria natans)、金鱼藻( Ceratophyllum demersum)、伊乐藻( Elodea nuttalli)、马来眼子菜( Potamogeton malaianus))生长的影响.结果显示,对照组、草鱼组和鳊组沉水植物生物量分别下降19.2%、82.1%和16.6%,而鲫组沉水植物生物量却增长12.5%.4种沉水植物中,金鱼藻生物量增长最高,其中对照组、鳊组和鲫组

  8. Accumulation of trace metals by aquatic macrophytes and their possible use in phytoremediation techniques

    Directory of Open Access Journals (Sweden)

    Michaela Hillermannová

    2008-01-01

    Full Text Available The aim of the performed research was to obtain knowledge on the ability of aquatic plants naturally growing at a site to absorb trace metals contained in bottom sediments and surface water. Furthermore, we compared differences in the accumulation of trace metals by the individual groups of aquatic plants (submerged and emergent and assessed a possible use of the individual plant species in phytoremediation techniques. Representative samples of water, sediments and aquatic macrophytes were taken from three anthropogenically loaded streams in six monitoring cycles in several collection profiles differing in the distance from a source of contamination. The samples were analysed for the total content of selected trace metals (As, Cd, Pb, Al, Hg, Zn, Fe, Mn, Cr, Ni and Cu. For comparison, one profile at an unloaded site was sampled as well. The obtained results were subjected to multivariate statistical analysis of data. Increased contents of Fe, Al, Mn, Cr and Zn were detected in sediments and plant biomass at loaded sites, namely 2–3× higher than at the comparing site. The contents of metals in surface water samples were altogether below the detection limit of the analytical method. When evaluating the individual plant species, we can state that the lowest contents of metals were detected in shore species (reed canary grass Phalaroides arundinacea, wood club-rush Scirpus silvaticus and red dock Rumex aquaticus; plant species growing in the very water current (water star-wort Callitriche sp. and flote-grass Glyceria fluitans exhibited mean contents of metals. In species forming mats (Fontinalis antipyretica and Cladophora sp., these contents were several times higher as compared to the previous species. The results of the performed research show that one of important factors, which influence the accumulation of trace metals in plants, is their ecological group (emergent – submerged affiliation and the species classification within this group

  9. Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast

    National Research Council Canada - National Science Library

    Riesmeier, J.W; Willmitzer, L; Frommer, W.B

    1992-01-01

    ...‐symport, but so far no sucrose carrier gene has been identified. Using an engineered Saccharomyces cerevisiae strain, a cDNA from spinach encoding a sucrose carrier was identified by functional expression...

  10. A Textual Research on Spinach, an Alien Plant%菠菜入华考

    Institute of Scientific and Technical Information of China (English)

    石润宏

    2014-01-01

    菠菜原产伊朗即古波斯,大约于唐贞观年间经由尼泊尔人传入我国,现在是中国厨房的一种常见蔬菜。菠菜初名菠薐或波稜,这是一个西域国名颇陵的音译。菠菜在唐代栽培不广,文学作品没有表现,只在农书和本草类书籍中有记载。唐诗之所以没有提到菠菜,是因为菠菜在唐代始终是一种小众的菜蔬,食用者只是本草学家、炼丹服丹者、苦于面毒的人以及一些境况不佳的农民。宋代以后,随着菠菜的广泛栽培,地方志开始著录它,菠菜也开始出现在文人诗歌中,并被赋予了凌霜耐寒的坚忍品质,再加上苏轼、苏过父子等名家的题咏,就使得菠菜在中国的饮食文化中占据了一方小小的天地。%Spinach, an alien plant which came from Persia, the ancient Iran, is now a common vegetable in Chinese people�s kitchen. It was brought into China by Nepal envoys in Zhenguan period of the Tang Dynas-ty. At that time, spinach was called Boleng, the name was a transliteration of a western country Poling. Spinach wasn�t widely cultivated in the Tang Dynasty, so there was no literary works involved it, only the herbals and agricultural books had some records. After the Song Dynasty, with the propagation of spinach, local history books began to record it, and it began to appear in literary poetry, from then it has been given the character like hard and perseverance. Spinach is also a therapeutic herb, ZHANG Lei, a poet in Song Dynasty had con-sumed it to detoxicate Miandu. Miandu, which is produced by the production process of flour, is a kind of com-position bad for human health. Spinach has its place in the field of Chinese dietary culture, written in poems by SU Shi, SU Guo and other poets.

  11. Spatial and Temporal Factors Associated with an Increased Prevalence of Listeria monocytogenes in Spinach Fields in New York State

    Science.gov (United States)

    Weller, Daniel; Wiedmann, Martin

    2015-01-01

    While rain and irrigation events have been associated with an increased prevalence of foodborne pathogens in produce production environments, quantitative data are needed to determine the effects of various spatial and temporal factors on the risk of produce contamination following these events. This study was performed to quantify these effects and to determine the impact of rain and irrigation events on the detection frequency and diversity of Listeria species (including L. monocytogenes) and L. monocytogenes in produce fields. Two spinach fields, with high and low predicted risks of L. monocytogenes isolation, were sampled 24, 48, 72, and 144 to 192 h following irrigation and rain events. Predicted risk was a function of the field's proximity to water and roads. Factors were evaluated for their association with Listeria species and L. monocytogenes isolation by using generalized linear mixed models (GLMMs). In total, 1,492 (1,092 soil, 334 leaf, 14 fecal, and 52 water) samples were collected. According to the GLMM, the likelihood of Listeria species and L. monocytogenes isolation from soil samples was highest during the 24 h immediately following an event (odds ratios [ORs] of 7.7 and 25, respectively). Additionally, Listeria species and L. monocytogenes isolates associated with irrigation events showed significantly lower sigB allele type diversity than did isolates associated with precipitation events (P = monocytogenes contamination. Small changes in management practices (e.g., not irrigating fields before harvest) may therefore reduce the risk of L. monocytogenes contamination of fresh produce. PMID:26116668

  12. The Use of Some Morphological Traits for the Assessment of Genetic Diversity in Spinach (Spinacia Oleracea L. Landraces

    Directory of Open Access Journals (Sweden)

    Ebadi-Segheloo Asghar

    2014-12-01

    Full Text Available Investigation of native accessions of spinach (Spinacia oleracea L. would be aid in the development of new genetically improved varieties, so in this research 121 spinach landraces, collected from the various spinach growing areas of Iran, were evaluated to determine their diversity using several agro-morphological traits. High coefficients of variation (CV were recorded in fresh yield, leaf area and dry yield. Using principal component (PC analysis, the first three PCs with eigenvalues more than 0.9 contributed 80.56% of the variability among accessions. The first PC was related to leaf yield performance (fresh and dry yields, leaf numbers at flowering and lateral branches while the PC2 was related to leaf characteristic (leaf width, petiole length, petiole diameter and leaf area. The third PC was related to seed characteristic (seed yield and 1000-seed weight and was named as seed property component. The 121 spinach landraces were grouped into six clusters using cluster analysis. Each cluster had some specific characteristics of its own and the clusters I and II were clearly separated from clusters III and V and also from clusters IV and VI. The studied accessions are an important resource for the generation of a core collection of spinach in the world. The results of present research will support tasks of conservation and utilization of landraces in spinach breeding programs.

  13. Oxygen uptake from aquatic macrophyte decomposition from Piraju Reservoir (Piraju, SP, Brazil

    Directory of Open Access Journals (Sweden)

    I. Bianchini Jr.

    Full Text Available The kinetics of oxygen consumption related to mineralisation of 18 taxa of aquatic macrophytes (Cyperus sp, Azolla caroliniana, Echinodorus macrophyllus, Eichhornia azurea, Eichhornia crassipes, Eleocharis sp1, Eleocharis sp2, Hetereanthera multiflora, Hydrocotyle raniculoides, Ludwigia sp, Myriophyllum aquaticum, Nymphaea elegans, Oxycaryum cubense, Ricciocarpus natans, Rynchospora corymbosa, Salvinia auriculata, Typha domingensis and Utricularia foliosa from the reservoir of Piraju Hydroelectric Power Plant (São Paulo state, Brazil were described. For each species, two incubations were prepared with ca. 300.0 mg of plant (DW and 1.0 L of reservoir water sample. The incubations were maintained in the dark and at 20 ºC. Periodically the dissolved oxygen (DO concentrations were measured; the accumulated DO values were fitted to 1st order kinetic model and the results showed that: i high oxygen consumption was observed for Ludwigia sp (533 mg g-1 DW, while the lowest was registered for Eleocharis sp1 (205 mg g-1 DW mineralisation; ii the higher deoxygenation rate constants were verified in the mineralisation of A. caroliniana (0.052 day-1, H. raniculoides (0.050 day-1 and U. foliosa (0.049 day-1. The oxygen consumption rate constants of Ludwigia sp and Eleocharis sp2 mineralisation (0.027 day-1 were the lowest. The half-time of oxygen consumption varied from 9 to 26 days. In the short term, the detritus of E. macrophyllus, H. raniculoides, Ludwigia sp, N. elegans and U. foliosa were the critical resources to the reservoir oxygen demand; while in the long term, A. caroliniana, H. multiflora and T. domingensis were the resources that can potentially contribute to the benthic oxygen demand of this reservoir.

  14. Oxygen uptake from aquatic macrophyte decomposition from Piraju Reservoir (Piraju, SP, Brazil).

    Science.gov (United States)

    Bianchini Jr, I; Cunha-Santino, M B; Panhota, R S

    2011-02-01

    The kinetics of oxygen consumption related to mineralisation of 18 taxa of aquatic macrophytes (Cyperus sp, Azolla caroliniana, Echinodorus macrophyllus, Eichhornia azurea, Eichhornia crassipes, Eleocharis sp1, Eleocharis sp2, Hetereanthera multiflora, Hydrocotyle raniculoides, Ludwigia sp, Myriophyllum aquaticum, Nymphaea elegans, Oxycaryum cubense, Ricciocarpus natans, Rynchospora corymbosa, Salvinia auriculata, Typha domingensis and Utricularia foliosa) from the reservoir of Piraju Hydroelectric Power Plant (São Paulo state, Brazil) were described. For each species, two incubations were prepared with ca. 300.0 mg of plant (DW) and 1.0 L of reservoir water sample. The incubations were maintained in the dark and at 20 ºC. Periodically the dissolved oxygen (DO) concentrations were measured; the accumulated DO values were fitted to 1st order kinetic model and the results showed that: i) high oxygen consumption was observed for Ludwigia sp (533 mg g-1 DW), while the lowest was registered for Eleocharis sp1 (205 mg g-1 DW) mineralisation; ii) the higher deoxygenation rate constants were verified in the mineralisation of A. caroliniana (0.052 day-1), H. raniculoides (0.050 day-1) and U. foliosa (0.049 day-1). The oxygen consumption rate constants of Ludwigia sp and Eleocharis sp2 mineralisation (0.027 day-1) were the lowest. The half-time of oxygen consumption varied from 9 to 26 days. In the short term, the detritus of E. macrophyllus, H. raniculoides, Ludwigia sp, N. elegans and U. foliosa were the critical resources to the reservoir oxygen demand; while in the long term, A. caroliniana, H. multiflora and T. domingensis were the resources that can potentially contribute to the benthic oxygen demand of this reservoir.

  15. Macrophyte growth in a pilot-scale constructed wetland for industrial wastewater treatment.

    Science.gov (United States)

    Hadad, H R; Maine, M A; Bonetto, C A

    2006-06-01

    A pilot-scale wetland was constructed to assess the feasibility of treating the wastewater from a tool industry in Santo Tomé, Santa Fe, Argentina. The wastewater had high conductivity and pH, and contained Cr, Ni and Zn. This paper describes the growth of vegetation in the experimental wetland and the nutrient and metal removal. The wetland was 6 x 3 x 0.4 m. Water discharge was 1000 l d(-1) and residence time was 7d. After the wetland was rendered impermeable, macrophytes from Middle Paraná River floodplain were transplanted. Influent and effluent quality was analyzed every 15 d. TP, Cr, Ni and Zn concentrations in leaves, roots and sediment (inlet and outlet) were measured monthly. Cover and biomass of predominant species were estimated. Also, greenhouse experiments were carried out to measure the effects of conductivity and pH on floating species. The variables measured in the influent were significantly higher than those in the effluent, except for HCO(3)(-) and NH(4)(+). TP and metal concentrations in sediment at the inlet were significantly higher than those at the outlet. Conductivity and pH of the incoming wastewater were toxic for the floating species. Typha domingensis displaced the other species and reached positive relative cover rate and biomass greater than those at the undisturbed natural environment. T. domingensis proved to be highly efficient for the treatment of wastewater. For that reason, it is the advisable species for the treatment of wastewater of high conductivity and pH enriched with metals, characteristic of many industrial processes.

  16. Modifications of the chromophore of Spinach aptamer based on QM:MM calculations.

    Science.gov (United States)

    Skúpa, Katarína; Urban, Ján

    2017-02-01

    Spinach aptamer was developed as an RNA analog of the green fluorescent protein. The aptamer interacts with its ligand and modifies its electronic spectrum so that it fluoresces brightly at the wavelength of 501 nm. Song et al. investigated modifications of the ligand in their experimental study and found a molecule emitting at 523 nm upon creating a complex with the Spinach aptamer. The crystal structure of the aptamer in complex with its original ligand has been published, which enabled us to study the system computationally. In this article, we suggest several new modifications of the ligand that shift the emission maximum of the complex to even longer wavelengths. Our results are based on combined quantum mechanical/molecular mechanical calculations with DFT method used for geometry optimization and TD-DFT for calculations of absorption and emission energies.

  17. Fat Metabolism in Higher Plants: LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts.

    Science.gov (United States)

    Jacobson, B S; Jaworski, J G; Stumpf, P K

    1974-10-01

    Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were either ascorbate, dichlorophenolindophenol, photosystem I and light, or NADPH and ferredoxin-NADP reductase. The desaturase was more active in extracts prepared from chloroplasts obtained from immature spinach leaves than from mature leaves. Stearyl-acyl carrier protein desaturase also occurs in soluble extracts of avocado (Persea americana Mill.) mesocarp and of developing safflower (Carthamus tinctorius) seeds.

  18. Enhancement of iron content in spinach plants stimulated by magnetic nano particles

    Science.gov (United States)

    Yulianto, Agus; Astuti, Budi; Amalia, Saptaria Rosa

    2016-04-01

    In our previous study, the iron content in spinach plants could be detected by magnetic susceptibility values. In the present work, magnetic nano particles were found from the iron sand. The magnetic nano particles are synthesis by using co-precipitation process and sol-gel technique. The stimulation of magnetic nano particles in the plant has been done by the provision of magnetic nano particles in growing media. After certain time, plant samples was characterized using susceptibility-meter MS2B and atomic absorption spectroscopy to measure the magnetic susceptibility and the amount of iron content that absorbed of the plant, respectively. The iron content in the spinach plants was increased when the magnetic nano particles was injected in the growing media.

  19. Multicolor FISH analysis of rDNA and telomere on spinach

    Institute of Scientific and Technical Information of China (English)

    Tianying LAN; Bo LIU; Fengping DONG; Ruiyang CHEN; Xiulan LI; Chengbin CHEN

    2008-01-01

    In this study,multicolor fluorescence in situ hybridization (FISH) analysis on metaphase chromosomes of spinach with biotin-labeled 25S rDNA,DIG-labeled telomere sequences and biotin-labeled and DIG-labeled 5S rDNA was performed.There were six 25S rDNA loci located on the satellites of the third,the fifth and the sixth chromosomes,and four 5S rDNA loci located on the long arms of the third and the fifth chromosomes.The telomere loci were located on the end of the sixth chromosome and also on both the end and centromeric regions of other chromosomes.This study is an important complement to both traditional karyotype analysis and FISH karyotype analysis in spinach.

  20. Effects of CeCl3 on Energy Transfer and Oxygen Evolution in Spinach Photosystem Ⅱ

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Due to 4f electron characteristics and alternation valence, cerium involved in an oxidation-reduction reaction in plant, closely relating to photosynthesis. Our studies proved that cerium could promote photosynthesis and greatly improve spinach growth. However, the mechanism of promoting energy transfer and conversion by cerium remains unclear. Here we reported that the effects of Ce3+ on energy transfer and oxygen evolution in photosystem Ⅱ(PSⅡ) isolated from spinach, which was related to 4f electron characteristics and alternation valence in Ce molecule. The methods of absorption spectrum, fluorescence spectrum were used in the research. Results showed that Ce3+ treatment at low concentration could suitably change PSⅡ microenvironment, increase the absorbance of visible light, improve the energy transfer among amino acids within PSⅡ protein-pigment complex, and accelerate energy transport from tyrosine residue to chlorophyll a. In summary, the photochemical activity of PSⅡ(fluorescence quantum yield) and its oxygen evolving rate were enhanced by Ce3+.

  1. STATISTIC MODELING OF DRYING KINETHIC OF SPINACH LEAVES USING MICROWAVE AND HOT AIR METHODS

    Directory of Open Access Journals (Sweden)

    Mojtaba Nouri

    2015-06-01

    Full Text Available The target of this study was to model of spinach leaves drying using microwave and hot air dryer. This test performed in combination treatment of temperatures (50°C, 60°C, and 70°C and microwave (90, 180, 360, 600 and 900w in 3 replications. Sample moisture measured within drying. All the results were fitted and analyzed with 8 mathematical models base on 3 parameters including determination (R2, Chi square(X2, root mean square errors(RSME. Results also revealed that temperature and microwave power effectively reduce the drying time when increase. Drying occurs in degrading stage; moreover the comparison of results exhibited that Page and Two sentences models were fitted appropriately to estimate moisture changing and drying description. Regarding all the results, it is cleared that microwave method is an appropriate method in spinach drying as a result of reducing drying temperature and its high efficiency.

  2. Assessment of heavy metal accumulation in macrophyte, agricultural soil, and crop plants adjacent to discharge zone of sponge iron factory

    Science.gov (United States)

    Gupta, S.; Nayek, S.; Saha, R. N.; Satpati, S.

    2008-08-01

    The present study deals with the characterization of effluent released from sponge iron industries and distribution of heavy metals in soil and macrophytes near to effluent discharge channel. Apart from this, accumulation of heavy metals in nearby soil and vegetation system irrigated with effluent-contaminated water is also the subject of this study. Physico-chemical analysis of effluent reveals that the concentration of total suspended solids (TSS), total hardness (TH), iron (Fe2+), and oil and grease are greater than the IS (1981) norms for discharge of water into inland water body. The soil along the sides of the effluent channel also shows higher concentration of heavy metals than the background soil. The enrichment of the heavy metals are in the order of Chromium (Cr) > Iron (Fe) > Manganese (Mn) > Zinc (Zn) > Copper (Cu) > Cadmium (Cd). Macrophytes growing along the sides of the effluent channel also show significant accumulation of heavy metals almost in the same order as accumulated in soil. Higher uptake of heavy metals by these varieties reveals that these species can be used for future phytoremediation. The effluent as well as contaminated water is extensively used for irrigation for growing vegetables like tomato ( Lycopersicon esculatum) in the surrounding areas. Heavy metal accumulation in this agricultural soil are in the sequence of Cr > Fe > Mn > Zn > Cu > Cd. More or less similar type of accumulation pattern are also found in tomato plants except Fe and Zn exceeding Cr and Mn. Transfer Factor of heavy metals from soil to tomato plants (TFS) shows average value of <1, suggesting less uptake of heavy metals from soil. Among the plant parts studied, fruit shows least accumulation. Although tomato plants show some phenotypic changes, the survival of tomato plants as well as least accumulation of metals in fruit reveals their tolerance to heavy metals. Therefore it may be suggested that this plant can be grown successfully in the heavy metal

  3. Climate-related differences in the dominance of submerged macrophytes in shallow lakes

    NARCIS (Netherlands)

    Kosten, S.; Kamarainen, A.; Jeppesen, E.; Nes, van E.H.; Peeters, E.T.H.M.; Lacerot, G.; Scheffer, M.

    2009-01-01

    It has been suggested that shallow lakes in warm climates have a higher probability of being turbid, rather than macrophyte dominated, compared with lakes in cooler climates, but little field evidence exists to evaluate this hypothesis. We analyzed data from 782 lake years in different climate zones

  4. Bioprospecting of Marine Macrophytes Using MS-Based Lipidomics as a New Approach

    Directory of Open Access Journals (Sweden)

    Elisabete Maciel

    2016-03-01

    Full Text Available The marine environment supports a remarkable diversity of organisms which are a potential source of natural products with biological activities. These organisms include a wide variety of marine plants (from micro- to macrophytes, which have been used in the food and pharmaceutical industry. However, the biochemistry and biological activities of many of these macrophytes (namely macroalgae and halophytes, including seagrasses are still far from being fully explored. Most popular bioactive components include polysaccharides, peptides, phenolics and fatty acids (FAs. Polar lipids (glycolipids, phospholipids and betaine lipids are emerging as novel value-added bioactive phytochemicals, rich in n-3 FA, with high nutritional value and health beneficial effects for the prevention of chronic diseases. Polar lipids account various combinations of polar groups, fatty acyl chains and backbone structures. The polar lipidome of macrophytes is remarkably diverse, and its screening represents a significant analytical challenge. Modern research platforms, particularly mass spectrometry (MS-based lipidomic approaches, have been recently used to address this challenge and are here reviewed. The application of lipidomics to address lipid composition of marine macrophytes will contribute to the stimulation of further research on this group and foster the exploration of novel applications.

  5. Archaea Dominate the Ammonia-Oxidizing Community in the Rhizosphere of the Freshwater Macrophyte Littorella uniflora

    DEFF Research Database (Denmark)

    Herrmann, Martina; Saunders, Aaron M.; Schramm, Andreas

    2008-01-01

    Archaeal and bacterial ammonia monooxygenase genes (amoA) had similar low relative abundances in freshwater sediment. In the rhizosphere of the submersed macrophyte Littorella uniflora, archaeal amoA was 500- to >8,000-fold enriched compared to bacterial amoA, suggesting that the enhanced nitrifi...... nitrification activity observed in the rhizosphere was due to ammonia-oxidizing Archaea....

  6. Ambiguous climate impacts on the competition between submerged macrophytes and phytoplankton in shallow lakes

    NARCIS (Netherlands)

    Kosten, S.; Jeppesen, E.; Huszar, V.M.; Mazzeo, N.; Nes, van E.H.; Peeters, E.T.H.M.; Scheffer, M.

    2011-01-01

    1. Shallow lakes may switch from a state dominated by submerged macrophytes to a phytoplankton-dominated state when a critical nutrient concentration is exceeded. We explore how climate change may affect this critical nutrient concentration by linking a graphical model to data from 83 lakes along a

  7. Ecotypic differentiation in thermal traits in the tropical to warm-temperate green macrophyte Valonia utricularis

    NARCIS (Netherlands)

    Eggert, A.; Burger, E.M.; Breeman, Arno

    2003-01-01

    Differentiation of thermal traits (i.e. growth, survival and reproduction) was studied in the green macrophyte Valonia utricularis (Roth) C. Agardh, which has a world-wide tropical to warm-temperate distribution. Ecotypic differentiation between northeast Atlantic/Mediterranean and Indo-west Pacific

  8. Bioprospecting of Marine Macrophytes Using MS-Based Lipidomics as a New Approach.

    Science.gov (United States)

    Maciel, Elisabete; Costa Leal, Miguel; Lillebø, Ana Isabel; Domingues, Pedro; Domingues, Maria Rosário; Calado, Ricardo

    2016-03-08

    The marine environment supports a remarkable diversity of organisms which are a potential source of natural products with biological activities. These organisms include a wide variety of marine plants (from micro- to macrophytes), which have been used in the food and pharmaceutical industry. However, the biochemistry and biological activities of many of these macrophytes (namely macroalgae and halophytes, including seagrasses) are still far from being fully explored. Most popular bioactive components include polysaccharides, peptides, phenolics and fatty acids (FAs). Polar lipids (glycolipids, phospholipids and betaine lipids) are emerging as novel value-added bioactive phytochemicals, rich in n-3 FA, with high nutritional value and health beneficial effects for the prevention of chronic diseases. Polar lipids account various combinations of polar groups, fatty acyl chains and backbone structures. The polar lipidome of macrophytes is remarkably diverse, and its screening represents a significant analytical challenge. Modern research platforms, particularly mass spectrometry (MS)-based lipidomic approaches, have been recently used to address this challenge and are here reviewed. The application of lipidomics to address lipid composition of marine macrophytes will contribute to the stimulation of further research on this group and foster the exploration of novel applications.

  9. Interactions between piscivores, zooplanktivores and zooplankton in submerged macrophytes : Preliminary observations from enclosure and pond experiments

    DEFF Research Database (Denmark)

    Jacobsen, Lene; Perrow, M.R.; Landkildehus, F.

    1997-01-01

    behavioural changes of zooplanktivores has received little attention, even though this may be an important mechanism in enhancing the stability of submerged macrophytes in shallow lakes. Preliminary observations from an unreplicated large-scale field enclosure experiment and a replicated pond experiment...

  10. A microcosm system to evaluate the toxicity of the triazine herbicide simazine on aquatic macrophytes

    Energy Technology Data Exchange (ETDEWEB)

    Vervliet-Scheebaum, Marco, E-mail: marco.vervliet@biologie.uni-freiburg.d [Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr. 1, 79104 Freiburg (Germany); Straus, Alain [Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr. 1, 79104 Freiburg (Germany); Tremp, Horst [Institute for Environmental Sciences, University Koblenz-Landau, Fortstr. 7, 76829 Landau (Germany); Hamer, Mick [Ecological Sciences, Syngenta Crop Protection AG, Jealott' s Hill International Research Centre, Bracknell, Berkshire RG42 6EY (United Kingdom); Maund, Stephen J. [Ecological Sciences, Syngenta Crop Protection AG, 4002 Basel (Switzerland); Wagner, Edgar [Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr. 1, 79104 Freiburg (Germany); Schulz, Ralf [Institute for Environmental Sciences, University Koblenz-Landau, Fortstr. 7, 76829 Landau (Germany)

    2010-02-15

    This study evaluates the effects of the triazine herbicide simazine in an outdoor pond microcosm test system that contained two submerged rooted species (Myriophyllum spicatum and Elodea canadensis) and two emergent rooted species (Persicaria amphibia and Glyceria maxima) over a period of 84 days. Simazine was applied to the microcosms at nominal concentrations of 0.05, 0.5 and 5 mg/L. General biological endpoints and physiological endpoints were used to evaluate herbicide toxicity on macrophytes and the algae developing naturally in the system. Concentration-related responses of macrophytes and algae were obtained for the endpoints selected, resulting in a no observed ecologically adverse effect concentration (NOEAEC) at simazine concentrations of 0.05 mg active ingredient/L after 84 days. E. canadensis was the most negatively affected species based on length increase, which was consistently a very sensitive parameter for all macrophytes. The experimental design presented might constitute a suitable alternative to conventional laboratory single-species testing. - Simazine at concentrations of 0.05 mg/L does not cause long-term negative effects to aquatic macrophytes or algae.

  11. Aquatic macrophyte and macroinvertebrate diversity and conservation in wetlands of the Sinos River basin.

    Science.gov (United States)

    Maltchik, L; Rolon, A S; Stenert, C

    2010-12-01

    Wetlands are important sites for biological conservation because they support rich biodiversity and present high productivity. Species-area relationship is an important tool in conservation planning and it has been extensively used for wetland management. This study had as aims: (1) to analyse macrophyte and macroinvertebrate diversity in the fragmented wetlands of the Sinos River basin; and (2) to investigate whether wetland areas could work as a tool for selecting the important habitats for biodiversity conservation. Throughout the study, 56 species of macrophytes and 57 taxa of macroinvertebrates were identified. Macrophyte richness was related to the wetland area, but macroinvertebrate richness, however, was not related to the wetland area. The macrophyte and macroinvertebrate composition were not related to the wetland area. Species composition varied between the regions of the basin and the difference in the species composition occurred mainly by the distribution of macroinvertebrates. With regard to the biodiversity conservation of the Sinos River basin, the wetland area must not be a priority criterion for choosing the important wetlands for conservation. The environmental policies for biodiversity conservation must include management actions focused also on the protection of small wetlands. Moreover, other criteria should be analysed in further research such as habitat diversity, hydroperiod, geographic distribution and connectivity.

  12. Aquatic Plant Control Research Program. Effects of Organic Amendments to Sediment on Freshwater Macrophyte Growth.

    Science.gov (United States)

    1983-10-01

    The growth of three submersed macrophyte species (Myriophyllum spicatum, Hyjdri11a verticillata, and Elodea canadensis) and three partially emergent... Elodea canadensis Rich. in Michx; and Hydrilla verticillata (L.f.) Caspary. Nomenclature follows Godfrey and Wooten (1979, 1981). The first two species

  13. Effect of lighting conditions of coastal zone of Knyaginya lake on composition of macrophyte biohydrocenoses

    Directory of Open Access Journals (Sweden)

    B. O. Baranovsky

    2005-10-01

    Full Text Available In articlе the stuffs of researches of influence of a mode of illuminating intensity of coastal zone of a different exposition flood-land of lake Knyaginya (valley Samara on composition of highest aqueous green and macrozoobentos macrophytes biogeocenose are submitted.

  14. Interaction between the macrophyte Stratiotes aloides and filamentous algae: does it indicate allelopathy?

    NARCIS (Netherlands)

    Mulderij, G.; Mau, B.; De Senerpont Domis, L.N.; Smolders, A.J.P.; Van Donk, E.

    2009-01-01

    The aquatic macrophyte Stratiotes aloides Linnaeus, which has recently received attention in studies on allelopathy, has been shown to suppress phytoplankton growth. In the Netherlands, S. aloides often co-occurs with floating filamentous algae. However, filamentous algae are generally absent in

  15. A simple equation for describing the temperature dependent growth of free-floating macrophytes

    NARCIS (Netherlands)

    Heide, van Tj.; Roijackers, R.M.M.; Nes, van E.H.; Peeters, E.T.H.M.

    2006-01-01

    Temperature is one of the most important factors determining growth rates of free-floating macrophytes in the field. To analyse and predict temperature dependent growth rates of these pleustophytes, modelling may play an important role. Several equations have been published for describing temperatur

  16. Removal of personal care compounds from sewage sludge in reed bed container (lysimeter) studies - Effects of macrophytes

    DEFF Research Database (Denmark)

    Chen, Xijuan; Pauly, Udo; Rehfus, Stefan

    2009-01-01

    as on the bactericide Triclosan. Additionally, the capacity of different macrophytes species to affect the treatment process was examined. Three different macrophyte species were compared: bulrush (Typha latifolia), reed (Phragmites australis) and reed canary grass (Phalaris arundinacea). They were planted...... into containers (lysimeters) with a size of 1 m × 1 m × 1 m which were filled with 20 cm gravel at the bottom and 50 cm sludge on top, into which the macrophytes were planted. During the twelve months experiment reduction of 20-30% for HHCB and AHTN, 70% for Triclosan and 70% for OTNE were determined under...

  17. Detection of Salmonella Typhimurium on Spinach Using Phage-Based Magnetoelastic Biosensors

    Directory of Open Access Journals (Sweden)

    Fengen Wang

    2017-02-01

    Full Text Available Phage-based magnetoelastic (ME biosensors have been studied as an in-situ, real-time, wireless, direct detection method of foodborne pathogens in recent years. This paper investigates an ME biosensor method for the detection of Salmonella Typhimurium on fresh spinach leaves. A procedure to obtain a concentrated suspension of Salmonella from contaminated spinach leaves is described that is based on methods outlined in the U.S. FDA Bacteriological Analytical Manual for the detection of Salmonella on leafy green vegetables. The effects of an alternative pre-enrichment broth (LB broth vs. lactose broth, incubation time on the detection performance and negative control were investigated. In addition, different blocking agents (BSA, Casein, and Superblock were evaluated to minimize the effect of nonspecific binding. None of the blocking agents was found to be superior to the others, or even better than none. Unblocked ME biosensors were placed directly in a concentrated suspension and allowed to bind with Salmonella cells for 30 min before measuring the resonant frequency using a surface-scanning coil detector. It was found that 7 h incubation at 37 °C in LB broth was necessary to detect an initial spike of 100 cfu/25 g S. Typhimurium on spinach leaves with a confidence level of difference greater than 95% (p < 0.05. Thus, the ME biosensor method, on both partly and fully detection, was demonstrated to be a robust and competitive method for foodborne pathogens on fresh products.

  18. Detection of Salmonella Typhimurium on Spinach Using Phage-Based Magnetoelastic Biosensors.

    Science.gov (United States)

    Wang, Fengen; Horikawa, Shin; Hu, Jiajia; Wikle, Howard C; Chen, I-Hsuan; Du, Songtao; Liu, Yuzhe; Chin, Bryan A

    2017-02-16

    Phage-based magnetoelastic (ME) biosensors have been studied as an in-situ, real-time, wireless, direct detection method of foodborne pathogens in recent years. This paper investigates an ME biosensor method for the detection of Salmonella Typhimurium on fresh spinach leaves. A procedure to obtain a concentrated suspension of Salmonella from contaminated spinach leaves is described that is based on methods outlined in the U.S. FDA Bacteriological Analytical Manual for the detection of Salmonella on leafy green vegetables. The effects of an alternative pre-enrichment broth (LB broth vs. lactose broth), incubation time on the detection performance and negative control were investigated. In addition, different blocking agents (BSA, Casein, and Superblock) were evaluated to minimize the effect of nonspecific binding. None of the blocking agents was found to be superior to the others, or even better than none. Unblocked ME biosensors were placed directly in a concentrated suspension and allowed to bind with Salmonella cells for 30 min before measuring the resonant frequency using a surface-scanning coil detector. It was found that 7 h incubation at 37 °C in LB broth was necessary to detect an initial spike of 100 cfu/25 g S. Typhimurium on spinach leaves with a confidence level of difference greater than 95% (p < 0.05). Thus, the ME biosensor method, on both partly and fully detection, was demonstrated to be a robust and competitive method for foodborne pathogens on fresh products.

  19. CONTENT OF MERCURY AND LEAD IN LEAVES OF SPINACH (SPINACIA OLERACEA, L.

    Directory of Open Access Journals (Sweden)

    Tomáš Tóth

    2014-02-01

    Full Text Available Spinach is one the most nutritious leafy vegetable. Only 22 calories per 100 grams, a high amount of protein, carbohydrate and fat make it very suitable ingredient in reduction diets. For a wealth of minerals and vitamins in addition it has an extraordinary nutritional value. 100 g contains two-thirds of its daily requirement of vitamin A, virtually the entire daily need for folic acid, half the dose of vitamin C, almost a quarter of the daily needs of more than a quarter of the needs of iron and magnesium. According to some studies, it is a good remedy for cancer prevention. In spinach are found in high concentrations of carotenoids, which protect cells from destruction, and so help to antiaging. Lutein-carotenoided pigment, an adequate supply may delay the emergence of degenerative diseases of the retina of the eye. In the framework of monitoring, we have focused on the analysis of the levels of lead and mercury in 7 samples of spinach from the commercial network of the Slovak republic. The lead content in all samples was 0.00 mg.kg-1, mercury content was in the range of 0.0019 – 0.0054 mg.kg-1. From the point of view of the sanitary evaluation of the HPQ for the content of lead and Hg was not exceeded in either sample.

  20. Detection of Salmonella Typhimurium on Spinach Using Phage-Based Magnetoelastic Biosensors

    Science.gov (United States)

    Wang, Fengen; Horikawa, Shin; Hu, Jiajia; Wikle, Howard C.; Chen, I-Hsuan; Du, Songtao; Liu, Yuzhe; Chin, Bryan A.

    2017-01-01

    Phage-based magnetoelastic (ME) biosensors have been studied as an in-situ, real-time, wireless, direct detection method of foodborne pathogens in recent years. This paper investigates an ME biosensor method for the detection of Salmonella Typhimurium on fresh spinach leaves. A procedure to obtain a concentrated suspension of Salmonella from contaminated spinach leaves is described that is based on methods outlined in the U.S. FDA Bacteriological Analytical Manual for the detection of Salmonella on leafy green vegetables. The effects of an alternative pre-enrichment broth (LB broth vs. lactose broth), incubation time on the detection performance and negative control were investigated. In addition, different blocking agents (BSA, Casein, and Superblock) were evaluated to minimize the effect of nonspecific binding. None of the blocking agents was found to be superior to the others, or even better than none. Unblocked ME biosensors were placed directly in a concentrated suspension and allowed to bind with Salmonella cells for 30 min before measuring the resonant frequency using a surface-scanning coil detector. It was found that 7 h incubation at 37 °C in LB broth was necessary to detect an initial spike of 100 cfu/25 g S. Typhimurium on spinach leaves with a confidence level of difference greater than 95% (p foodborne pathogens on fresh products. PMID:28212322

  1. Recognising macrophyte-vegetated environments in the rock record: a new criterion using 'hooked' forms of crustose coralline red algae

    Science.gov (United States)

    Beavington-Penney, Simon J.; Paul Wright, V.; Woelkerling, Wm. J.

    2004-04-01

    The role of macrophytes (such as seagrasses) in influencing deposition in shallow marine environments has long been understood. They affect sediment textures such that they do not reflect ambient hydrodynamic conditions, and so the recognition of ancient vegetated environments is important for accurate palaeoenvironmental analysis. However, there is little direct fossil evidence of such plants in the rock record, and so the presence of former macrophyte cover generally has to be inferred from indirect evidence based on the characteristics of modern macrophyte-vegetated environments. We present a new criterion for the recognition of vegetated environments in the rock record, based upon a distinctive 'hooked' form of crustose coralline red algae that is definitively indicative of growth on seagrasses (and also on other macrophytes with similarly flattened blades), and has been recognised from both modern (Inhaca Island, Mozambique) and fossil (the Eocene of Oman) seagrass beds.

  2. Macrophytes may not contribute significantly to removal of nutrients, pharmaceuticals, and antibiotic resistance in model surface constructed wetlands.

    Science.gov (United States)

    Cardinal, Pascal; Anderson, Julie C; Carlson, Jules C; Low, Jennifer E; Challis, Jonathan K; Beattie, Sarah A; Bartel, Caitlin N; Elliott, Ashley D; Montero, Oscar F; Lokesh, Sheetal; Favreau, Alex; Kozlova, Tatiana A; Knapp, Charles W; Hanson, Mark L; Wong, Charles S

    2014-06-01

    Outdoor shallow wetland mesocosms, designed to simulate surface constructed wetlands to improve lagoon wastewater treatment, were used to assess the role of macrophytes in the dissipation of wastewater nutrients, selected pharmaceuticals, and antibiotic resistance genes (ARGs). Specifically, mesocosms were established with or without populations of Typha spp. (cattails), Myriophyllum sibiricum (northern water milfoil), and Utricularia vulgaris (bladderwort). Following macrophyte establishment, mesocosms were seeded with ARG-bearing organisms from a local wastewater lagoon, and treated with a single pulse of artificial municipal wastewater with or without carbamazepine, clofibric acid, fluoxetine, and naproxen (each at 7.6μg/L), as well as sulfamethoxazole and sulfapyridine (each at 150μg/L). Rates of pharmaceutical dissipation over 28d ranged from 0.073 to 3.0d(-1), corresponding to half-lives of 0.23 to 9.4d. Based on calculated rate constants, observed dissipation rates were consistent with photodegradation driving clofibric acid, naproxen, sulfamethoxazole, and sulfapyridine removal, and with sorption also contributing to carbamazepine and fluoxetine loss. Of the seven gene determinants assayed, only two genes for both beta-lactam resistance (blaCTX and blaTEM) and sulfonamide resistance (sulI and sulII) were found in sufficient quantity for monitoring. Genes disappeared relatively rapidly from the water column, with half-lives ranging from 2.1 to 99d. In contrast, detected gene levels did not change in the sediment, with the exception of sulI, which increased after 28d in pharmaceutical-treated systems. These shallow wetland mesocosms were able to dissipate wastewater contaminants rapidly. However, no significant enhancement in removal of nutrients or pharmaceuticals was observed in mesocosms with extensive aquatic plant communities. This was likely due to three factors: first, use of naïve systems with an unchallenged capacity for nutrient assimilation and

  3. Morphometry and retention time as forcing functions to establishment and maintenance of aquatic macrophytes in a tropical reservoir

    OpenAIRE

    M. B. Cunha-Santino; Fushita,A. T.; PERET, A.C.; Bianchini-Junior,I.

    2016-01-01

    Abstract Macrophytes may constitute an important resource for several chemical, physical and biological processes within aquatic ecosystems. This study considers that in tropical reservoirs with low retention time and with low values of shoreline development (DL), the expansion and persistence of aquatic macrophytes are mainly reported to local conditions (e.g., hydrodynamic and wind exposure) rather than trophic status and depth of the euphotic zone. In this context, this study aimed at desc...

  4. Decreasing but still significant facilitation effect of cold-season macrophytes on wetlands purification function during cold winter

    OpenAIRE

    Xiangxu Zou; Hui Zhang; Jie Zuo; Penghe Wang; Dehua Zhao; Shuqing An

    2016-01-01

    To identify the facilitation effect of a cool-season aquatic macrophyte (FEam) for use in effluent purification via constructed floating wetlands (CFWs) and to determine the possible pathways used during a winter period with an average temperature of less than 5??C, pilot-scale CFWs were planted with the cold-season macrophyte Oenanthe clecumbens and were operated as batch systems. Although some leaves withered, the roots retained relatively high levels of activity during the winter, which ha...

  5. Growth and nutrient removal of three macrophytes in response to concentrations and ratios of N and P.

    Science.gov (United States)

    Liao, JianXiong; Zhang, DeNan; Mallik, Azim; Huang, YuQing; He, ChengXin; Xu, GuangPing

    2017-01-13

    Wastewater from different sources show great differences in concentrations and ratios of N and P. In order to choose suitable plant species to remove excess N and/or P from polluted waters, it is important to know the performances of these plants under different N and P concentrations. In this study, we investigated the growth and N and P removal of three macrophytes, Coix lacryma-jobi, Iris wilsonii and Arundo donax under six N and P combination treatments. C. lacryma-jobi preferred higher N and P concentrations (16 mg N L(-1) and 3.2 mg P L(-1)), and increasing N supply could increase its P removal rate. I. wilsonii exhibited a growth preference at a combination of moderate N and P concentrations (8 mg N L(-1) and 0.8 mg P L(-1)). A. donax could grow well at all combinations of N and P and had significantly higher relative growth rate (RGR) and N and P removal rates than the other two species. These results showed A. donax is a promising species to treat various polluted waters and the other two species can be used specifically to treat certain types of wastewater.

  6. Concentrations of heavy metals and aquatic macrophytes of Govind Ballabh Pant Sagar an anthropogenic lake affected by coal mining effluent.

    Science.gov (United States)

    Mishra, Virendra Kumar; Upadhyay, Alka Rani; Pandey, Sudhir Kumar; Tripathi, B D

    2008-06-01

    Five heavy metals Cu, Cd, Mn, Pb and Hg were found in high concentration from three sampling sites located in Asia's largest anthropogenic lake Govind Ballabh Pant GBP Sagar. Concentrations of these heavy metals were measured in Water, bottom sediment and in different parts of the aquatic macrophytes collected from the reservoir. Plants collected from the lake were Eichhornia crassipes, Azolla pinnata, Lemna minor, Spirodela polyrrhiza, Potamogeton pectinatus, Marsilea quadrifolia, Pistia stratiotes, Ipomea aquqtica, Potamogeton crispus, Hydrilla verticillata and Aponogeton natans. These plants have shown the high concentrations of Cu, Cd, Mn, Pb and Hg in their different parts due to bioaccumulation. In general plant roots exhibited higher concentrations of heavy metals than corresponding sediments. A comparison between different morphological tissues of the sampled plants revealed the metal concentration in following order roots > leaves. Analyses of bottom sediment indicated the higher concentrations of Cd, Mn, Cu and Pb. Strong positive correlations were obtained between the metals in water and in plants as well as between metal in sediment and in plants. Indicating the potential of these plants for pollution monitoring of these metals.

  7. Scale-dependent changes in the functional diversity of macrophytes in subtropical freshwater lakes in south China.

    Science.gov (United States)

    Fu, Hui; Zhong, Jiayou; Fang, Shaowen; Hu, Jianmin; Guo, Chunjing; Lou, Qian; Yuan, Guixiang; Dai, Taotao; Li, Zhongqiang; Zhang, Meng; Li, Wei; Xu, Jun; Cao, Te

    2017-08-15

    Ecological processes are generally scale-dependent and there is little consensus about the relative importance of deterministic versus stochastic processes in driving patterns of biological diversity. We investigated how the relationship between functional dispersion and environmental gradients changes with spatial scale in subtropical freshwater lakes. The functional alpha and beta dispersions of all the tested traits were significantly under-dispersed across spatial scales and along environmental gradients. Results showed more functional similarity within communities in leaf dry mass content and flowering duration but less functional turnover among communities in all the tested traits at regional scales (Yunnan-Guizhou plateau and the middle and low reaches of the Yangtze River). The strengths and directions of environmental effects on the functional alpha and beta dispersions depended on the selected traits, diversity metrics and spatial scales. Surprisingly, broad-scale factors - elevation and water transparency - decreased the functional turnover for most traits along the gradients, whereas fine-scale factors - water depth - produced the opposite patterns along the gradient, depending on the trait selected. Our study highlights the dominant role of deterministic assembly processes in structuring the local functional composition and governing the spatial functional turnover of macrophyte communities across multiple spatial scales.

  8. Oxidative response of wetland macrophytes in response to contaminants of abiotic components of East Kolkata wetland ecosystem

    Directory of Open Access Journals (Sweden)

    Pal Sudin

    2014-06-01

    Full Text Available The tannery effluent and composite municipal sewage water drained to the East Kolkata wetland (EKW, a Ramsar Site (1208, is used for agriculture and pisciculture after natural stabilization. Such composite wastewater is characterized by exceedingly high total dissolved solids, total hardness, chloride and heavy metals concentrations. These water born pollutants generate reactive oxygen species which are potentially toxic to the biological system. These reactive oxygen species are normally detoxified by some enzymes, such as superoxide dismutase (SOD and catalase (CAT. The present study was commenced to find out the SOD and CAT activities against the oxidative stress, if any, in four macrophytes namely, Eichhornia crassipes, Pistia stratiotes, Alternanthera sessilis and Sagittarria montevidensis of contaminated ponds (Site 1 and Site 2 of EKW and an uncontaminated site (Control site. During the course of sampling the physico-chemical factors were found significantly higher in the EKW ponds compared to the control site. In the EKW sites, higher rate of evaporation during summer months caused higher elemental concentration in the premonsoon than in other seasons. This led to high activity of both SOD and CAT enzymes. In contrast, heavy rain fall in monsoon lowers the elemental concentration - mainly due to dilution effect. Present experiment indicated that in a stressed ecosystem like EKW, the wetland plants overcome the stress by altering their stress enzyme activities, hence suggesting an evidence of adaptive mechanism to thrive in a stressful environment.

  9. Effects of different sewage sludge applications on heavy metal accumulation, growth and yield of spinach (Spinacia oleracea L.).

    Science.gov (United States)

    Eid, Ebrahem M; El-Bebany, Ahmed F; Alrumman, Sulaiman A; Hesham, Abd El-Latif; Taher, Mostafa A; Fawy, Khaled F

    2017-04-03

    In this study, we present the response of spinach to different amendment rates of sewage sludge (0, 10, 20, 30, 40 and 50 g kg(-1)) in a greenhouse pot experiment, where plant growth, biomass and heavy metal uptake were measured. The results showed that sewage sludge application increased soil electric conductivity (EC), organic matter, chromium and zinc concentrations and decreased soil pH. All heavy metal concentrations of the sewage sludge were below the permissible limits for land application of sewage sludge recommended by the Council of the European Communities. Biomass and all growth parameters (except the shoot/root ratio) of spinach showed a positive response to sewage sludge applications up to 40 g kg(-1) compared to the control soil. Increasing the sewage sludge amendment rate caused an increase in all heavy metal concentrations (except lead) in spinach root and shoot. However, all heavy metal concentrations (except chromium and iron) were in the normal range and did not reach the phytotoxic levels. The spinach was characterized by a bioaccumulation factor heavy metal transport to the edible parts (shoot) because the TFs for all heavy metals (except zinc) were <1.0. In conclusion, sewage sludge used in the present study can be considered for use as a fertilizer in spinach production systems in Saudi Arabia, and the results can serve as a management method for sewage sludge.

  10. Cold Plasma-activated hydrogen peroxide aerosol inactivates Escherichia coli 0157:H7, Salmonella Typhimurium, and Listeria innocua and maintains quality of grape tomato, spinach and cantaloupe

    Science.gov (United States)

    The purpose of this study was to investigate the efficacy of aerosolized hydrogen peroxide in inactivating bacteria and maintaining quality of grape tomato, baby spinach leaves and cantaloupe. Stem scar and smooth surfaces of tomatoes, spinach leaves, and cantaloupe rinds, inoculated with Escherich...

  11. Use of partial least squares discriminant analysis on visible-near infrared multispectral image data to examine germination ability and germ length in spinach seeds

    DEFF Research Database (Denmark)

    Shetty, Nisha; Olesen, Merete Halkjær; Gislum, René;

    2012-01-01

    Because of the difficulties in obtaining homogenous germination of spinach seeds for baby leaf production, the possibility of using partial least squares discriminant analysis (PLS-DA) on features extracted from multispectral images of spinach seeds was investigated. The objective has been...

  12. Aquatic macrophytes in natural and managed wetlands of Rio Grande do Sul State, Southern Brazil Macrófitas aquáticas em áreas úmidas naturais e manejadas do Rio Grande do Sul, sul do Brasil

    Directory of Open Access Journals (Sweden)

    Ana Silvia Rolon

    2010-06-01

    Full Text Available AIM: This study gathers the main results obtained from studies regarding dynamic of aquatic macrophyte community in natural and managed wetlands of Southern Brazil. We analyzed the aquatic macrophytes diversity in wetlands of Southern Brazil, the environmental factors that determine the structure of the aquatic macrophyte community in fragmented wetlands, the effects of floods on the dynamics of macrophytes, and the contributions to the rice field for the conservation of aquatic macrophytes; METHODS: The information was obtained from several researches carried in several spatial scales and different wetlands types over the last 10 years in Southern Brazil; RESULTS: The studies have reported the occurrence of approximately 250 species of aquatic macrophytes. Wetland area, habitat diversity, altitude and hydroperiod were determinant for macrophyte richness and composition in wetlands of Southern Brazil. Furthermore, flood events, long or short-term ones, are strongly associated to the structure of the aquatic macrophyte community. The rice field systems of Southern Brazil (crops and irrigation channel shelter a representative number of species of macrophyte found at natural wetlands in this region. The agricultural practices adopted over rice cultivation cycle in the rice fields have influenced the macrophyte richness and biomass. The different hydrological management practices adopted after the harvesting period (presence or lack of water surface did not influence the macrophyte richness and biomass, however it influenced the species composition; CONCLUSIONS: The increasing process of wetland degradation (e.g. fragmentation, flood control and rice field expansion presents a threat to the conservation aquatic macrophyte species.OBJETIVO: Este estudo reúne os principais resultados obtidos em trabalhos sobre a dinâmica da comunidade de macrófitas aquáticas em áreas úmidas naturais e manejadas do sul do Brasil. Nós analisamos a diversidade de

  13. Physiological response of the three submerged macrophytes to the five cadmium concentrations%3种沉水植物对镉的生理特性响应

    Institute of Scientific and Technical Information of China (English)

    易冕; 张饮江; 董悦; 刘晓培; 李岩; 金晶; 段婷; 张乐亭

    2013-01-01

    This research is aimed at providing a theoretical guidance and experimental basis for the application of submerged macrophytes to treat the effects of heavy-metal sewage pollution on the five concentrations of cadmium(Cd) to the biomass, the growth rate, the chlorophyll content, the chlorophyll activities and the soluable protein of Ceratophyllum demersum L. , Elodea nuttallii and Vallisneria natans through imitating the static and shallow water environment. For our research purpose, we have chosen the well grown Ceratophyllum demersum L. , Elodea nuttallii and Vallisneria natans as the material to be tested, with the 1/4 Hoagland liquid as the culture medium on the basis for determinating the Cd test concentration in the preliminary tests. We have conducted our formal tests lasting for 40 days in hoping to find the biomass, relative growth rate, the chlorophyll content and chlorophyll activity of the above said three submerged macro-phytes under different Cd stresses. The results show that the effects for treating turn to be different due to the different concentrations of cadmium (Cd) used and the different sampling times. It was found that the biomass, the relative growth rate and the chlorophyll contents of the three submerged macrophytes prove to noticeably decrease with the increase of cadmium (Cd) concentration, but the chlorophyll activity and the soluble protein content tend to increase first and then decrease. We have also found that the chlorophyll activities and soluble protein content of Ceratophyllum Demersum L. would reach the highest point with the Cd concentration being 10 μg/L and 5 μg/L respectively, but the Elodea nuttallii being the same as Ceratophyllum Demersum L. . Moreover, when the chlorophyll activity and the soluble protein content of Vallisneria natans reached the highest degree with the Cd concentration being 5 μg/L and 10 μg/L respectively, the concentration change of cadmium(Cd) was supposed to affect the growth of the three

  14. Emergent macrophytes select for nitrifying and denitrifying microorganisms in constructed wetlands

    Science.gov (United States)

    Trias, Rosalia; Ramió Pujol, Sara; Bañeras, Lluis

    2014-05-01

    The use of constructed wetlands for wastewater treatment is a reliable low-cost alternative that has been widely developed during the last years. Several processes involving plants, sediments, and microbial communities contribute to nitrogen removal in wetlands. Vegetation plays an important role in this process, not only by nutrient assimilation but also by the stimulation of the plant associated microbiota. Plants supply oxygen at the close proximity of the root surface that may favour ammonia oxidizers. At the same time, exudation of organic compounds potentially speeds-up denitrification in the anoxic environment. The aim of this work was to understand the plant-microbe interactions at the root level in the Empuriabrava free water surface constructed wetland (Spain). The roots of the macrophytes Typha latifolia, Typha angustifolia, Phragmites australis and Bolboschoenus maritimus were sampled at four dates from January to September 2012, covering all the stages of plant growth. Additionally, sediment surrounding vegetation and non-vegetated sediments were sampled. Microbial community structure was analysed by pyrosequencing of bacterial and archaeal 16S rDNA and functional genes (nirK, nirS, nosZ and amoA). Bacterial communities were significantly different in sediments of the vegetated areas compared to the root surface. Plant roots exhibited a higher proportion of proteobacteria whereas Actinobacteria were dominant in sediments. The nitrifiers Nitrosomonas sp. and Nitrosococcus sp. accounted for less than 1% of all sequences. Archaeal communities were dominated by the Miscellaneous Crenarchaeotic Groups C2 and C3 and Methanomicrobia. Higher relative abundances of MCG were found in roots of P. australis, B. maritimus and T. angustifolia. Ammonia oxidizing archaea accounted for less than 0.1% of all sequences but were consistently more abundant in sediment samples compared to roots. NirK and NirS-type bacterial communities showed clearly distinct distribution

  15. BIO-MONITORING FOR URANIUM USING STREAM-SIDE TERRESTRIAL PLANTS AND MACROPHYTES

    Energy Technology Data Exchange (ETDEWEB)

    Caldwell, E.; Duff, M.; Hicks, T.; Coughlin, D.; Hicks, R.; Dixon, E.

    2012-01-12

    This study evaluated the abilities of various plant species to act as bio-monitors for environmental uranium (U) contamination. Vegetation and soil samples were collected from a U processing facility. The water-way fed from facility storm and processing effluents was the focal sample site as it represented a primary U transport mechanism. Soils and sediments from areas exposed to contamination possessed U concentrations that averaged 630 mg U kg{sup -1}. Aquatic mosses proved to be exceptional accumulators of U with dry weight (dw) concentrations measuring as high as 12500 mg U kg{sup -1} (approximately 1% of the dw mass was attributable to U). The macrophytes (Phragmites communis, Scripus fontinalis and Sagittaria latifolia) were also effective accumulators of U. In general, plant roots possessed higher concentrations of U than associated upper portions of plants. For terrestrial plants, the roots of Impatiens capensis had the highest observed levels of U accumulation (1030 mg kg{sup -1}), followed by the roots of Cyperus esculentus and Solidago speciosa. The concentration ratio (CR) characterized dry weight (dw) vegetative U levels relative to that in associated dw soil. The plant species that accumulated U at levels in excess of that found in the soil were: P. communis root (CR, 17.4), I. capensis root (CR, 3.1) and S. fontinalis whole plant (CR, 1.4). Seven of the highest ten CR values were found in the roots. Correlations with concentrations of other metals with U were performed, which revealed that U concentrations in the plant were strongly correlated with nickel (Ni) concentrations (correlation: 0.992; r-squared: 0.984). Uranium in plant tissue was also strongly correlated with strontium (Sr) (correlation: 0.948; r-squared: 0.899). Strontium is chemically and physically similar to calcium (Ca) and magnesium (Mg), which were also positively-correlated with U. The correlation with U and these plant nutrient minerals, including iron (Fe), suggests that active

  16. Polyphenols with algicidal activity in the submerged macrophyte Myriophyllum Spicatum L.

    OpenAIRE

    Gross, Elisabeth; Sütfeld, Rainer

    1994-01-01

    The role of phenols as defence mechanisms in various terrestrial plants is well studied. However, in aquatic environments, they are probably involved also in the chemical interactions between species. A periodic shift from dominance by submerged macrophytes to dominance by phytoplankton and vice versa without apparent change in the external nutrient loading is sometimes observed in shallow eutrophic lakes. Due to the fact that nutrient levels are not usually limiting to phytoplankton growth, ...

  17. Aquiatic macrophytes in Doñana protected area (SW Spain).

    OpenAIRE

    García Murillo, Pablo; Fernández Zamudio, María del Rosario; Cirujano Bracamonte, Santos; Sousa Martín, Arturo

    2006-01-01

    A big portion of the Doñana protected areas corresponds to wetlands; in them aquatic macrophytes are the main primary producers and play also other important ecological functions. Nevertheless, they are inconspicuous organisms and their importance in these ecosystems does not seem to be well reflected in the bibliography about this natural area. This paper reviews the most significant information gathered about this group of organisms in this protected area, provides an updated catal...

  18. Native macrophyte density and richness affect the invasiveness of a tropical poaceae species.

    Directory of Open Access Journals (Sweden)

    Thaisa S Michelan

    Full Text Available The role of the native species richness and density in ecosystem invasibility is a matter of concern for both ecologists and managers. We tested the hypothesis that the invasiveness of Urochloa arrecta (non-native in the Neotropics is negatively affected by the species richness and abundance of native aquatic macrophytes in freshwater ecosystems. We first created four levels of macrophyte richness in a greenhouse (richness experiment, and we then manipulated the densities of the same native species in a second experiment (density experiment. When the native macrophytes were adults, fragments of U. arrecta were added, and their growth was assessed. Our results from the richness experiment corroborated the hypothesis of a negative relationship between the native species richness and the growth of U. arrecta, as measured by sprout length and root biomass. However, the resistance to invasion was not attributed to the presence of a particular native species with a greater competitive ability. In the density experiment, U. arrecta growth decreased significantly with an increased density of all five of the native species. Density strongly affected the performance of the Poaceae in a negative manner, suggesting that patches that are densely colonized by native macrophytes and less subject to disturbances will be more resistant to invasion than those that are poorly colonized and more commonly subjected to disturbances. Our density experiment also showed that some species exhibit a higher competitive ability than others (sampling effect. Although native richness and abundance clearly limit the colonization and establishment of U. arrecta, these factors cannot completely prevent the invasion of aquatic ecosystems by this Poaceae species.

  19. Native macrophyte density and richness affect the invasiveness of a tropical poaceae species.

    Science.gov (United States)

    Michelan, Thaisa S; Thomaz, Sidinei M; Bini, Luis M

    2013-01-01

    The role of the native species richness and density in ecosystem invasibility is a matter of concern for both ecologists and managers. We tested the hypothesis that the invasiveness of Urochloa arrecta (non-native in the Neotropics) is negatively affected by the species richness and abundance of native aquatic macrophytes in freshwater ecosystems. We first created four levels of macrophyte richness in a greenhouse (richness experiment), and we then manipulated the densities of the same native species in a second experiment (density experiment). When the native macrophytes were adults, fragments of U. arrecta were added, and their growth was assessed. Our results from the richness experiment corroborated the hypothesis of a negative relationship between the native species richness and the growth of U. arrecta, as measured by sprout length and root biomass. However, the resistance to invasion was not attributed to the presence of a particular native species with a greater competitive ability. In the density experiment, U. arrecta growth decreased significantly with an increased density of all five of the native species. Density strongly affected the performance of the Poaceae in a negative manner, suggesting that patches that are densely colonized by native macrophytes and less subject to disturbances will be more resistant to invasion than those that are poorly colonized and more commonly subjected to disturbances. Our density experiment also showed that some species exhibit a higher competitive ability than others (sampling effect). Although native richness and abundance clearly limit the colonization and establishment of U. arrecta, these factors cannot completely prevent the invasion of aquatic ecosystems by this Poaceae species.

  20. COMPARISON OF METHODS TO DETECT ALLELOPATHIC EFFECTS OF SUBMERGED MACROPHYTES ON GREEN ALGAE(1).

    Science.gov (United States)

    Hilt Nee Körner, Sabine; Beutler, Eike; Bauer, Nadine

    2012-02-01

    Detecting allelopathic inhibition of phytoplankton by submerged macrophytes in an ecologically meaningful way is not easy. Multiple-approach investigations from a laboratory scale to the ecosystem level have been recommended to overcome the shortcomings of individual methods. Whether results of different methods are qualitatively or quantitatively comparable has not yet been tested. Here, we compare the sensitivity of the green algae Desmodesmus subspicatus (Chodat) E. Hegewald et Ant. Schmidt and Stigeoclonium helveticum Vischer to the allelopathic effect of the submerged macrophyte Myriophyllum verticillatum L. The following three approaches were used: (i) coincubation of algae in dialysis membrane tubes in a lake inside and outside a M. verticillatum stand, (ii) coincubation of algae in dialysis membrane tubes in aquaria with and without M. verticillatum, and (iii) single additions of tannic acid (TA), an allelopathically active polyphenol present in this macrophyte, to the algae cultures. For each method, fluorescence-based (chl a, PSII activity) and particle-based (cell count, biovolume) parameters were compared after 48 h of incubation. Results revealed quantitative and qualitative differences between methods. Algae incubated in dialysis membrane tubes in aquaria showed a strong decrease in all parameters under the influence of macrophytes. In situ measurements were influenced by adverse growth conditions for the test algae and only detected significant reductions for biovolume. Single additions of TA induced a strong reduction of fluorescence-based parameters similar to aquarium results, but an increase in the cell count. Even the qualitative transfer of laboratory results to field conditions thus requires caution and a proper selection of parameters.

  1. Ocean Acidification Differentially Affects the Photosynthesis of Key New England Macrophytes

    Science.gov (United States)

    Fachon, E.; Ets-Hokin, J. M.; Donham, E. M.; Price, N.

    2016-02-01

    While the influence of anthropogenic CO2 emissions on seawater chemistry is detrimental to calcification among CaCO3 reliant organisms such as commercially important shellfish species, non-calcareous macrophytes like seagrasses and seaweeds can experience enhanced growth under elevated pCO2 conditions and may be a substantial, if ephemeral, CO2 sink. Most marine macrophytes rely on enzyme conversion of HCO3- to supply the inorganic carbon necessary for photosynthesis; the ability to down-regulate this energetically expensive carbon acquisition under high pCO2 conditions could determine future species success. We exposed four commercially and ecologically relevant New England macrophytes (Saccharina latissima, Fucus vesiculosus, Ulva lactuca, and Zostera marina) to pre-industrial (280 uatm), present (400 and 520 utam - recorded in Casco Bay) and future (640, 880 and 1120 uatm - as predicted by the IPCC) pCO2 levels in 1.5 hr long respirometry assays after 72 hrs acclimation. CO2 consumption, photosynthetic quotient (Q = CO2 consumed:O2 evolved), and change in carbonate saturation state (Ωcalcite) were calculated for each sample using differences in initial and final carbonate chemistry and dissolved oxygen concentrations. All species experienced increases in rate of CO2 uptake and Q under elevated pCO2 treatments, but response level differed across species. Saccharina latissima had the greatest relative effect on all parameters measured, consuming 4 times more carbon at high pCO2 levels than the lowest performing species. While all macrophytes were able to raise Ωcalcite, the magnitude of change decreased at higher pCO2 levels, suggesting a limitation to the degree to which photosynthesis can locally raise calcification potential for sensitive native or farmed populations of shellfish in the future. The varied responses observed across species have implications for future community structures and for phytoremediation efforts.

  2. Toxicity of Zinc on Growth of an Aquatic Macrophyte, Ipomoea Aquatica Forsk

    OpenAIRE

    Laitonjam Bedabati Chanu; Abhik Gupta

    2016-01-01

    The effects of different concentrations of Zn on growth of an aquatic macrophyte, Ipomoea aquatica Forsk. were studied. Fresh weight, dry weight, shoot length, root length, number of nodes, development of leaves, and chlorophyll and carotene contents were the different growth parameters considered. Toxicity symptoms like browning and decaying of roots could be observed in plants treated at 22.7 mg L-1 Zn as early as 3rd day of experiment while yellowing of older leaves appeared during the lat...

  3. Dye-sensitized solar cell using natural dyes extracted from spinach and ipomoea

    Energy Technology Data Exchange (ETDEWEB)

    Chang, H., E-mail: f10381@ntut.edu.t [Department of Mechanical Engineering, National Taipei University of Technology, No. 1. Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Wu, H.M. [Department of Materials Engineering, Tatung University, No. 40, Sec. 3, Jhongshan N. Rd. Jhongshan District, Taipei City 104, Taiwan (China); Chen, T.L. [Department of Industrial Design, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Huang, K.D. [Department of Vehicle Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Jwo, C.S. [Department of Energy and Air-Conditioning Refrigeration Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China); Lo, Y.J. [Department of Mechanical Engineering, National Taipei University of Technology, No. 1. Sec. 3, Chung-Hsiao E. Rd., Taipei 10608, Taiwan (China)

    2010-04-16

    This study used spinach extract, ipomoea leaf extract and their mixed extracts as the natural dyes for a dye-sensitized solar cell (DSSC). Spinach and ipomoea leaves were first placed separately in ethanol and the chlorophyll of these two kinds of plants was extracted to serve as the natural dyes for using in DSSCs. In addition, the self-developed nanofluid synthesis system prepared a TiO{sub 2} nanofluid with an average particle size of 50 nm. Electrophoresis deposition was performed to let the TiO{sub 2} deposit nanoparticles on the indium tin oxide (ITO) conductive glass, forming a TiO{sub 2} thin film with the thickness of 11.61 {mu}m. This TiO{sub 2} thin film underwent sintering at 450 {sup o}C to enhance the compactness of thin film. Finally, the sintered TiO{sub 2} thin film was immersed in the natural dye solutions extracted from spinach and ipomoea leaves, completing the production of the anode of DSSC. This study then further inspected the fill factor, photoelectric conversion efficiency and incident photon current efficiency of the encapsulated DSSC. According to the experimental results of current-voltage curve, the photoelectric conversion efficiency of the DSSCs prepared by natural dyes from ipomoea leaf extract is 0.318% under extraction temperature of 50 {sup o}C and pH value of extraction fluid at 1.0. This paper also investigated the influence of the temperature in the extraction process of this kind of natural dye and the influence of pH value of the dye solution on the UV-VIS patterns absorption spectra of the prepared natural dye solutions, and the influence of these two factors on the photoelectric conversion efficiency of DSSC.

  4. Comparison of two possible routes of pathogen contamination of spinach leaves in a hydroponic cultivation system.

    Science.gov (United States)

    Koseki, Shigenobu; Mizuno, Yasuko; Yamamoto, Kazutaka

    2011-09-01

    The route of pathogen contamination (from roots versus from leaves) of spinach leaves was investigated with a hydroponic cultivation system. Three major bacterial pathogens, Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes, were inoculated into the hydroponic solution, in which the spinach was grown to give concentrations of 10⁶ and 10³ CFU/ml. In parallel, the pathogens were inoculated onto the growing leaf surface by pipetting, to give concentrations of 10⁶ and 10³ CFU per leaf. Although contamination was observed at a high rate through the root system by the higher inoculum (10⁶ CFU) for all the pathogens tested, the contamination was rare when the lower inoculum (10³ CFU) was applied. In contrast, contamination through the leaf occurred at a very low rate, even when the inoculum level was high. For all the pathogens tested in the present study, the probability of contamination was promoted through the roots and with higher inoculum levels. The probability of contamination was analyzed with logistic regression. The logistic regression model showed that the odds ratio of contamination from the roots versus from the leaves was 6.93, which suggested that the risk of contamination from the roots was 6.93 times higher than the risk of contamination from the leaves. In addition, the risk of contamination by L. monocytogenes was about 0.3 times that of Salmonella enterica subsp. enterica serovars Typhimurium and Enteritidis and E. coli O157:H7. The results of the present study indicate that the principal route of pathogen contamination of growing spinach leaves in a hydroponic system is from the plant's roots, rather than from leaf contamination itself.

  5. Impact of Selenium Supplementation on Growth and Selenium Accumulation on Spinach (Spinacia oleracea L. Plants

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    Azadeh SAFFARYAZDI

    2012-11-01

    Full Text Available Selenium (Se has been proved to be an essential element for humans and animals. However, less is known about its effects on plants. A hydroponic experiment was carried out to investigate the effects of selenium on growth, selenium accumulation and some physiological characteristics of spinach (Spinacia oleracea L. cv. Missouri plants. Plants were grown in Hoagland nutrient solution amended with sodium selenite at 0 (control, 1, 2, 4, 6 and 10 mg.L-1 for 28 days. Growth parameters like shoot and root fresh weight, shoot and root dry weight, total dry weight, shoot and root length increased by 17, 15, 38, 19, 18 and 34 percent in response to the lowest concentration of Se (1 mg L-1, respectively over control. However, application of higher Se concentrations reduced these parameters as compared to control. Selenium up to 1 mg L-1 enhanced the levels of chlorophyll a and chlorophyll b by 87 and 165 percent, respectively, while higher levels of Se exert toxic effects. Total phenolic compounds in leaves increased directly by increasing the level of Se and plants treated with 10 mg. L-1 Se had the highest values. Selenium, sodium and calcium content increased, while potassium content decreased, by increasing selenium treatments. The highest amounts of Se in shoots (3.89 mg g-1 DW and roots (4.27 mg g-1 DW were obtained for the highest concentration of Se (10 mg L-1. The present results suggested the beneficial effects of Se on spinach growth and also its contribute ion to improving the nutritional value of spinach for livestock and human nutrition.

  6. Characteristics of Light-Dependent Inorganic Carbon Uptake by Isolated Spinach Chloroplasts

    Science.gov (United States)

    Sicher, Richard C.

    1984-01-01

    The light-dependent accumulation of radioactively labeled inorganic carbon in isolated spinach (Spinacia oleracea L.) chloroplasts was determined by silicone oil filtering centrifugation. Intact chloroplasts, dark-incubated 60 seconds at pH 7.6 and 23°C with 0.5 millimolar sodium bicarbonate, contained 0.5 to 1.0 millimolar internal inorganic carbon. The stromal pool of inorganic carbon increased 5- to 7-fold after 2 to 3 minutes of light. The saturated internal bicarbonate concentration of illuminated spinach chloroplasts was 10- to 20-fold greater than that of the external medium. This ratio decreased at lower temperatures and with increasing external bicarbonate. Over one-half the inorganic carbon found in intact spinach chloroplasts after 2 minutes of light was retained during a subsequent 3-minute dark incubation at 5°C. Calculations of light-induced stromal alkalization based on the uptake of radioactively labeled bicarbonate were 0.4 to 0.5 pH units less than measurements performed with [14C]dimethyloxazolidine-dione. About one-third of the binding sites on the enzyme ribulose 1,5-bisphosphate carboxylase were radiolabeled when the enzyme was activated in situ and 14CO2 bound to the activator site was trapped in the presence of carboxypentitol bisphosphates. Deleting orthophosphate from the incubation medium eliminated inorganic carbon accumulation in the stroma. Thus, bicarbonate ion distribution across the chloroplast envelope was not strictly pH dependent as predicted by the Henderson-Hasselbach formula. This finding is potentially explained by the presence of bound CO2 in the chloroplast. PMID:16663542

  7. Long-term dietary strawberry, spinach, or vitamin E supplementation retards the onset of age-related neuronal signal-transduction and cognitive behavioral deficits.

    Science.gov (United States)

    Joseph, J A; Shukitt-Hale, B; Denisova, N A; Prior, R L; Cao, G; Martin, A; Taglialatela, G; Bickford, P C

    1998-10-01

    Recent research has indicated that increased vulnerability to oxidative stress may be the major factor involved in CNS functional declines in aging and age-related neurodegenerative diseases, and that antioxidants, e.g., vitamin E, may ameliorate or prevent these declines. Present studies examined whether long-term feeding of Fischer 344 rats, beginning when the rats were 6 months of age and continuing for 8 months, with diets supplemented with a fruit or vegetable extract identified as being high in antioxidant activity, could prevent the age-related induction of receptor-mediated signal transduction deficits that might have a behavioral component. Thus, the following parameters were examined: (1) oxotremorine-enhanced striatal dopamine release (OX-K+-ERDA), (2) cerebellar beta receptor augmentation of GABA responding, (3) striatal synaptosomal 45Ca2+ clearance, (4) carbachol-stimulated GTPase activity, and (5) Morris water maze performance. The rats were given control diets or those supplemented with strawberry extracts (SE), 9.5 gm/kg dried aqueous extract (DAE), spinach (SPN 6.4 gm/kg DAE), or vitamin E (500 IU/kg). Results indicated that SPN-fed rats demonstrated the greatest retardation of age-effects on all parameters except GTPase activity, on which SE had the greatest effect, whereas SE and vitamin E showed significant but equal protection against these age-induced deficits on the other parameters. For example, OX-K+-ERDA enhancement was four times greater in the SPN group than in controls. Thus, phytochemicals present in antioxidant-rich foods such as spinach may be beneficial in retarding functional age-related CNS and cognitive behavioral deficits and, perhaps, may have some benefit in neurodegenerative disease.

  8. Model based analysis of transient fluorescence yield induced by actinic laser flashes in spinach leaves and cells of green alga Chlorella pyrenoidosa Chick.

    Science.gov (United States)

    Belyaeva, N E; Schmitt, F-J; Paschenko, V Z; Riznichenko, G Yu; Rubin, A B; Renger, G

    2014-04-01

    Measurements of Single Flash Induced Transient Fluorescence Yield (SFITFY) on spinach leaves and whole cells of green thermophilic alga Chlorella pyrenoidosa Chick were analyzed for electron transfer (ET) steps and coupled proton transfer (PT) on both the donor and the acceptor side of the reaction center (RC) of photosystem II (PS II). A specially developed PS II model (Belyaeva et al., 2008, 2011a) allowed the determination of ET steps that occur in a hierarchically ordered time scale from nanoseconds to several seconds. Our study demonstrates that our SFITFY data is consistent with the concept of the reduction of P680(+) by YZ in both leaves and algae (studied on spinach leaves and cells of Chlorella pyrenoidosa Chick). The multiphasic P680(+) reduction kinetics by YZ in PS II core complexes with high oxygen evolution capacity was seen in both algae and leaves. Model simulation to fit SFITFY curves for dark adapted species used here gives the rate constants to verify nanosecond kinetic stages of P680(+) reduction by YZ in the redox state S1 of the water oxidizing complex (WOC) shown in Kühn et al. (2004). Then a sequence of relaxation steps in the redox state S1, outlined by Renger (2012), occurs in both algae and leaves as a similar non-adiabatic ET reactions. Coupled PT is discussed briefly to understand a rearrangement of hydrogen bond protons in the protein matrix of the WOC (Umena et al., 2011). On the other hand, present studies showed a slower reoxidation of reduced QA by QB in algal cells as compared with that in a leaf that might be regarded as a consequence of differences of spatial domains at the QB-site in leaves compared to algae. Our comparative study helped to correlate theory with experimental data for molecular photosynthetic mechanisms in thylakoid membranes. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  9. Microbial communities in marine sediments modify success of an invasive macrophyte.

    Science.gov (United States)

    Gribben, Paul E; Nielsen, Shaun; Seymour, Justin R; Bradley, Daniel J; West, Matthew N; Thomas, Torsten

    2017-08-29

    Invasive plants have extensive impacts on ecosystem function and biodiversity globally. Our inability to manage invasive species stems in part from a lack of understanding of the processes that control their successful establishment and spread. To date, studies have largely considered how above-ground processes control native/invasive plant interactions. Emerging research from terrestrial and wetland ecosystems demonstrates that below-ground processes under microbial control can determine the outcome of interactions between native and invasive plants. Whether sediment microbes modify the success of invasive macrophytes in marine ecosystems is untested, despite marine sediment microbes controlling many ecological processes (e.g. nutrient cycling) comparable to those in terrestrial ecosystems. We first show that sediment bacterial communities differ between the native seagrass Zostera capricorni and the invasive alga Caulerpa taxifolia and that those differences relate to functional changes in sulfur cycling between the macrophytes. Second, by experimentally manipulating the microbial communities we show that intact microbial communities in Z. capricorni sediments provide biotic resistance by reducing C. taxifolia fragment growth 119% compared to when they are inactive, and intact microbial communities in C. taxifolia sediments have positive feedbacks by increasing fragment growth 200%. Thus, similar to terrestrial ecosystems, microorganisms appear to indirectly control the success of invasive macrophytes in marine ecosystems.

  10. Habitat selection by three littoral zone fishes: effects of predation pressure, plant density and macrophyte type

    Science.gov (United States)

    Chick, J.H.; McIvor, C.C.

    1997-01-01

    We conducted laboratory experiments which demonstrated that three littoral zone fishes differentially selected among three macrophytes when seeking refuge from predation. In the presence of a predator (a juvenile Micropterus salmoides), mosquitofish (Gambusia holbrooki), sailfin mollies (Poecilia latipinna). and dollar sunfish (Lepomis marginatus) displayed ferential use of four tank areas containing patches of either Hydrilla verticillata, Potamogeton illinoensis, Panicum hemitomon, or no plants. Patterns habitat selection, and the consistency of these patterns among replicates, differed among the three fishes and among three plant-density treatments - natural (each macrophyte presented at its mean field density), equal (all three macrophytes at the same density), and control (no plants). Selection for H. verticillata by mosquitofish was significant for both the equal and natural treatments, and thus was not caused by differences in plant density alone. Sailfin mollies displayed significant selection for H. verticillata only in the natural plant-density treatments. Dollar sunfish showed less consistent habitat selection than either mosquitofish or sailfin mollies. Significant habitat selection was not found in the absence of a predator, and there was no evidence for lection among the tank areas in control treatments. Patterns of habitat selection by the three fishes in our laboratory study corresponded to observed habitat use in Lake Okeechobee.

  11. An invasion risk map for non-native aquatic macrophytes of the Iberian Peninsula

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    Argantonio Rodríguez-Merino

    2017-05-01

    Full Text Available Freshwater systems are particularly susceptible to non-native organisms, owing to their high sensitivity to the impacts that are caused by these organisms. Species distribution models, which are based on both environmental and socio-economic variables, facilitate the identification of the most vulnerable areas for the spread of non-native species. We used MaxEnt to predict the potential distribution of 20 non-native aquatic macrophytes in the Iberian Peninsula. Some selected variables, such as the temperature seasonality and the precipitation in the driest quarter, highlight the importance of the climate on their distribution. Notably, the human influence in the territory appears as a key variable in the distribution of studied species. The model discriminated between favorable and unfavorable areas with high accuracy. We used the model to build an invasion risk map of aquatic macrophytes for the Iberian Peninsula that included results from 20 individual models. It showed that the most vulnerable areas are located near to the sea, the major rivers basins, and the high population density areas. These facts suggest the importance of the human impact on the colonization and distribution of non-native aquatic macrophytes in the Iberian Peninsula, and more precisely agricultural development during the Green Revolution at the end of