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Sample records for m34 casamino acids-yeast

  1. Dethiosulfatibacter aminovorans gen. nov., sp. nov., a novel thiosulfate-reducing bacterium isolated from coastal marine sediment via sulfate-reducing enrichment with Casamino acids.

    Science.gov (United States)

    Takii, Susumu; Hanada, Satoshi; Tamaki, Hideyuki; Ueno, Yutaka; Sekiguchi, Yuji; Ibe, Akihiro; Matsuura, Katsumi

    2007-10-01

    A sulfate-reducing enrichment culture originating from coastal marine sediment of the eutrophic Tokyo Bay, Japan, was successfully established with Casamino acids as a substrate. A thiosulfate reducer, strain C/G2(T), was isolated from the enrichment culture after further enrichment with glutamate. Cells of strain C/G2(T) were non-motile rods (0.6-0.8 microm x 2.2-4.8 microm) and were found singly or in pairs and sometimes in short chains. Spores were not formed. Cells of strain C/G2(T) stained Gram-negatively, despite possessing Gram-positive cell walls. The optimum temperature for growth was 28-30 degrees C, the optimum pH was around 7.8 and the optimum salt concentration was 20-30 g l(-1). Lactate, pyruvate, serine, cysteine, threonine, glutamate, histidine, lysine, arginine, Casamino acids, peptone and yeast extract were fermented as single substrates and no sugar was used as a fermentative substrate. A Stickland reaction was observed with some pairs of amino acids. Fumarate, alanine, proline, phenylalanine, tryptophan, glutamine and aspartate were utilized only in the presence of thiosulfate. Strain C/G2(T) fermented glutamate to H2, CO2, acetate and propionate. Thiosulfate and elemental sulfur were reduced to sulfide. Sulfate, sulfite and nitrate were not utilized as electron acceptors. The growth of strain C/G2(T) on Casamino acids or glutamate was enhanced by co-culturing with Desulfovibrio sp. isolated from the original mixed culture enriched with Casamino acids. The DNA G+C content of strain C/G2(T) was 41.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C/G2(T) formed a distinct cluster with species of the genus Sedimentibacter. The closest relative was Sedimentibacter hydroxybenzoicus (with a gene sequence similarity of 91 %). On the basis of its phylogenetic and phenotypic properties, strain C/G2(T) (=JCM 13356(T)=NBRC 101112(T)=DSM 17477(T)) is proposed as representing a new genus and novel species, Dethiosulfatibacter

  2. The manometric determination of thiamine pyrophosphate and the inhibition of the acid yeast phosphatase

    NARCIS (Netherlands)

    Steyn-Parvé, Elizabeth P.

    1962-01-01

    Sodium molybdate is a powerful inhibitor of the acid yeast phosphatase in both fresh baker's yeast and dried brewer's yeast, provided that the yeast is suspended in a suitable buffer. It displays no action in citrate or phosphate buffers, but is active in acetate or maleate buffers, both at the opti

  3. Clinical evidence of the nonpathogenic nature of the M34T variant in the connexin 26 gene.

    Science.gov (United States)

    Feldmann, Delphine; Denoyelle, Françoise; Loundon, Natalie; Weil, Dominique; Garabedian, Erea-Noel; Couderc, Remy; Joannard, Alain; Schmerber, Sébastien; Delobel, Bruno; Leman, Jacques; Journel, Hubert; Catros, Hélène; Ferrec, Claude; Drouin-Garraud, Valérie; Obstoy, Marie-Françoise; Moati, Lucien; Petit, Christine; Marlin, Sandrine

    2004-04-01

    Mutations in GJB2 are the most common cause of congenital nonsyndromic hearing loss. The controversial allele variant M34T has been hypothesized to cause autosomal dominant or recessive nonsyndromic hearing impairment and some in vitro data has been consistent with this hypothesis. In this report, we present the clinical and genotypic study of 11 families (seven familial forms of nonsyndromic sensorineural hearing loss (NSSNHL) and four sporadic cases) in which the M34T GJB2 variant has been identified. The M34T mutation did not segregate with the deafness in six of the seven familial forms of NSSNH. Eight persons with normal audiogram presented a heterozygous M34T variation and five normal hearing individuals were composite heterozygous for M34T and another GJB2 mutation. Four normal hearing individuals with a documented audiogram were M34T/35delG and one was M34T/(GJB6-D13S1830)del. Screening a French control population of 116 subjects we have found an M34T allele frequency of 1.72%. This percentage was not significatively different from the prevalence of the M34T allele in the deaf population, which was 2.12%. All these data suggest that the M34T variant is not clinically significant in human and is a frequent polymorphism in France.

  4. 酪蛋白水解物对雷帕霉素产生菌Streptomyces hygroscopicus ATCC29253接合转移效率的影响%Effect of casamino acid on intergeneric conjugation in rapamycin-producing Streptomyces hygroscopicus ATCC29253

    Institute of Scientific and Technical Information of China (English)

    张万壵; 鲁慧囡; 何璟

    2012-01-01

    [ Objective] Streptomyces kygroscopicus ATCC29253 has attracted much interests due to its capacity of producing various secondary metabolites with strong bioactivities, including immunosuppressant rapamycin, nigericin, hexaenes, elaiophylin and hygroeins. [Objective] To investigate biosynthetic pathway of these metabolites and construct high-yield strains by genetic engineering, establishment of a highly efficient genetic manipulation system is critically required in this strain. [Methods] We tested the effects of conjugation media and donor strains on conjugal transfer from Esckerichia coli to 5. Hygroscopicus ATCC29253 and other Streptomycetes. [Results] We found that both casamino acid and MgCl2 supplemented in conjugation media improved conjugation frequency in S. Kygroscopicus ATCC29253. A random experiment led to the disclosure of an optimal combination of casamino acid and MgCl2 by which the conjugation frequency in 5. Kygroscopicus reached 1.5 × 10" . Meanwhile, we also found significant changes in conjugation frequencies of S. Lividans, S. Albus and S. Avermitilis when casamino acid was supplemented in conjugation media. [ Conclusion ] Casamino acid has significant influence on conjugation frequency in not only S. Hygroscopicus ATCC29253 but also other Streptomyces such as S. Lividans, S. Albus and S. Avermitilis.%吸水链霉菌(Streptomyces hygroscopicus)ATCC29253能够产生非常丰富的次级代谢产物,除了雷帕霉素外,还可以分泌尼日利亚菌素、洋橄榄叶素、六烯类抗生素等多种具有生物活性的物质,具有重要的研究价值和应用前景.[目的]而建立高效的遗传操作系统是研究该链霉菌相关代谢产物合成机理和构建基因工程菌株的基础.[方法]我们测试了不同培养基及供体菌对吸水链霉菌及其它链霉菌接合转移效率的影响.[结果]我们发现酪蛋白水解物和镁离子能显著提高S.hygroscopicus ATCC29253接合转移效率.通过随机组合

  5. Preparation of low nucleic acid yeast extract by a mesotherm/light-salt method%中温淡盐法制备低核酸酵母抽提物的工艺研究

    Institute of Scientific and Technical Information of China (English)

    王斌; 黄丽娜; 邱丽娟

    2014-01-01

    Using a novel mesotherm/light-salt method for the removal of nucleic acid , the high quality , low nucleic acid yeast extract was successfully prepared .This technique was simple and short in processing time under 70℃.By using low amount of sodium chlo-ride, desalting is not required.Moreover, content of nucleic acid was reduced by over 70%.Further more, the yeast product had a light color with a tasty meat flavor , suitable for food processing .In addition , this technique produced a high-yield high-purity nucleic acid byproduct .Mesotherm/light-salt technique broadened the application field of yeast extract products , provided a solution for obtai-ning safe and nutritious yeast products to individuals with uric acid in the high side or gout .%通过建立一种新的酵母核酸中温淡盐去除法,成功研制出品质优良的低核酸酵母抽提物。工艺简单,处理时间短,温度不超过70℃,可避免高温对酵母营养物质的破坏;该法氯化钠用量少,无需脱盐处理,易产业化及应用推广;研究制备的酵母抽提物,核酸去除量达70%以上,色浅、肉香味鲜,适合食品加工。同时,附加得到高提取率、高纯度的核酸。中温淡盐法去除酵母核酸的技术,扩展了酵母制品的应用领域,更为尿酸偏高及痛风人群食用安全营养的酵母食品提供了解决方案。

  6. Crianza Practica de Aves (Practical Poultry Raising). Appropriate Technologies for Development. Peace Corps Information Collection & Exchange Manual Series [No.] M-34.

    Science.gov (United States)

    French, Kenneth M.

    Written in Spanish, this manual is designed to provide development workers with the information and tools needed to begin or to improve poultry production. Covered in the individual chapters are the following topics: the nature and scope of poultry production, assessment of local poultry selections, basic information about chickens, country…

  7. Tindallia californiensis sp. nov., a new anaerobic, haloalkaliphilic, spore-forming acetogen isolated from Mono Lake in California

    Science.gov (United States)

    Pikuta, E. V.; Hoover, R. B.; Bej, A. K.; Marsic, D.; Detkova, E. N.; Whitman, W. B.; Krader, P.

    2003-01-01

    A novel extremely haloalkaliphilic, strictly anaerobic, acetogenic bacterium strain APO was isolated from sediments of the athalassic, meromictic, alkaline Mono Lake in California. The Gram-positive, spore-forming, slightly curved rods with sizes 0.55- 0.7x1.7-3.0 microns were motile by a single laterally attached flagellum. Strain APO was mesophilic (range 10-48 C, optimum of 37 C); halophilic (NaCl range 1-20% (w/v) with optimum of 3-5% (w/v), and alkaliphilic (pH range 8.0-10.5, optimum 9.5). The novel isolate required sodium ions in the medium. Strain APO was an organotroph with a fermentative type of metabolism and used the substrates peptone, bacto-tryptone, casamino acid, yeast extract, L-serine, L-lysine, L-histidine, L-arginine, and pyruvate. The new isolate performed the Stickland reaction with the following amino acid pairs: proline + alanine, glycine + alanine, and tryptophan + valine. The main end product of growth was acetate. High activity of CO dehydrogenase and hydrogenase indicated the presence of a homoacetogenic, non-cycling acetyl-coA pathway. Strain APO was resistant to kanamycin but sensitive to chloramphenicol, tetracycline, and gentamycin. The G+C content of the genomic DNA was 44.4 mol% (by HPLC method). The sequence of the 16s rRNA gene of strain APO possessed 98.2% similarity with the sequence from Tindullia magadiensis Z-7934, but the DNA-DNA hybridization value between these organisms was only 55%. On the basis of these physiological and molecular properties, strain APO is proposed to be a novel species of the genus Tindallia with the name Tindallia californiensis sp. nov., (type strain APO = ATCC BAA-393 - DSM 14871).

  8. Glucose significantly enhances enterotoxigenic Escherichia coli adherence to intestinal epithelial cells through its effects on heat-labile enterotoxin production.

    Directory of Open Access Journals (Sweden)

    Prageeth Wijemanne

    Full Text Available The present study tested whether exposure of enterotoxigenic Escherichia coli (ETEC to glucose at different concentrations in the media results in increased bacterial adherence to host cells through increased heat-labile enterotoxin (LT production, thereby suggesting the effects are physiological. Porcine-origin ETEC strains grown in Casamino acid yeast extract medium containing different concentrations of glucose were washed and inoculated onto IPEC-J2 porcine intestinal epithelial cells to test for effects on adherence and host cell cAMP concentrations. Consistent with previous studies, all LT+ strains had higher ETEC adherence to IPEC-J2 cells than did LT- strains. Adherence of the LT- but not the LT+ strains was increased by pre-incubating the IPEC-J2 cells with LT and decreased by co-incubation with GM1 ganglioside in a dose-dependent manner (P<0.05. To determine whether the glucose concentration of the cell culture media has an effect on adherence, IPEC-J2 cells were inoculated with LT+ or LT- strains in cell culture media containing a final glucose concentration of 0, 0.25, 0.5, 1.0 or 2.0%, and incubated for 4 h. Only media containing 0.25% glucose resulted in increased adherence and cAMP levels, and this was limited to IPEC-J2 cells inoculated with LT+ strains. This study supports the hypothesis that glucose, at a concentration optimal for LT expression, enhances bacterial adherence through the promotion of LT production. Hence, these results establish the physiological relevance of the effects of glucose on LT production and provide a basis for how glucose intake may influence the severity of ETEC infection.

  9. Tindallia Californiensis sp. nov.: A New Halo-Alkaliphilic Primary Anaerobe, Isolated from Meromictic soda Mono Lake in California and the Correction of Diagnosis for Genus Tindallia

    Science.gov (United States)

    Pikuta, Elena; Marsic, Damien; Hoover, Richard B.; Kevbrin, Vadim; Whitman, William B.; Krader, Paul; Cleland, Dave; Six, N. Frank (Technical Monitor)

    2002-01-01

    A novel extremely halo-alkaliphilic, bacterium strain APO (sup T) was isolated from sediments of the athalassic, meromictic, soda Mono Lake in California. Gram positive, spore-forming, slightly curved rods with sizes 0.6-0.7x 2.5-4.0 micrometers which occur singly, in pairs or short curved chains. Cells, are motile by singular subcentral flagellum. Strain APO (sup T) is mesophilic: growth was observed over the temperature range of +10 C to +48 C (optimum +37 C), NaCl concentration range 1-20 %, wt/vol (optimum 3-5%, wt/vol) and pH range 8.0-11.0 (optimum pH 9.5). The novel isolate is strictly halo-alkaliphilic, requires sodium chloride in medium, obligately anaerobic and catalase-negative. Strain APO (sup T) is organo-heterotroph with fermentative type of metabolism, and uses as substrates: peptone, badotryptone, casamino acids, yeast extract, L-serine, L-lysine, L-histidine, L-arginine, and pyruvate. The main end products of growth on peptone medium were: lactate, acetate, propionate, and ethanol. Strain APO (sup T) is resistant to kanamycin, but sensitive to chloramphenicol, tetracycline, and gentamycin. The sum of G+C in DNA is 44.4 mol% (by HPLC method). On the bait of physiological and molecular properties, the isolate was considered as novel species of genus Tindallia; and the name Tindallia californiensis sp. nov., is proposed for new isolate (type strain APO (sup T) - ATCC BAA_393(sup T) = DSMZ 14871 (sup T)).

  10. Tindallia Californiensis sp. nov.: A New Halo-Alkaliphilic Primary Anaerobe, Isolated from Meromictic soda Mono Lake in California and the Correction of Diagnosis for Genus Tindallia

    Science.gov (United States)

    Pikuta, Elena; Marsic, Damien; Hoover, Richard B.; Kevbrin, Vadim; Whitman, William B.; Krader, Paul; Cleland, Dave; Six, N. Frank (Technical Monitor)

    2002-01-01

    A novel extremely halo-alkaliphilic, bacterium strain APO (sup T) was isolated from sediments of the athalassic, meromictic, soda Mono Lake in California. Gram positive, spore-forming, slightly curved rods with sizes 0.6-0.7x 2.5-4.0 micrometers which occur singly, in pairs or short curved chains. Cells, are motile by singular subcentral flagellum. Strain APO (sup T) is mesophilic: growth was observed over the temperature range of +10 C to +48 C (optimum +37 C), NaCl concentration range 1-20 %, wt/vol (optimum 3-5%, wt/vol) and pH range 8.0-11.0 (optimum pH 9.5). The novel isolate is strictly halo-alkaliphilic, requires sodium chloride in medium, obligately anaerobic and catalase-negative. Strain APO (sup T) is organo-heterotroph with fermentative type of metabolism, and uses as substrates: peptone, badotryptone, casamino acids, yeast extract, L-serine, L-lysine, L-histidine, L-arginine, and pyruvate. The main end products of growth on peptone medium were: lactate, acetate, propionate, and ethanol. Strain APO (sup T) is resistant to kanamycin, but sensitive to chloramphenicol, tetracycline, and gentamycin. The sum of G+C in DNA is 44.4 mol% (by HPLC method). On the bait of physiological and molecular properties, the isolate was considered as novel species of genus Tindallia; and the name Tindallia californiensis sp. nov., is proposed for new isolate (type strain APO (sup T) - ATCC BAA_393(sup T) = DSMZ 14871 (sup T)).

  11. Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

    CSIR Research Space (South Africa)

    Bulani, S

    2012-05-01

    Full Text Available Z, Liu G-L, Wang F, Madzak C, Chi Z-M (2010) Inulin hydrolysis and citric acid production from inulin using the surface-engineered Y. lipolytica displayed inulase. Metabol Engineer 12:469–467 Madzak C, Blanchin-Roland S, Gaillardin C (1995) Upstream.... protein (GPI-CWP1). Yeast transformants were selected on YNB- casamino acid plates (0.17% YNB without amino acids and ammonium sulfate, 1% glucose, 0.1% casamino acids, 0.1% sodium glutamate and 1.5% agar). For expression of immobilised proteins on Y...

  12. An altered Pseudomonas diversity is recovered from soil by using nutrient-poor Pseudomonas-selective soil extract media

    DEFF Research Database (Denmark)

    Aagot, N.; Nybroe, O.; Nielsen, P.

    2001-01-01

    . Several of these analyses showed that the amount of Casamino Acids significantly influenced the diversity of the recovered Pseudomonas isolates. Furthermore, the data suggested that specific Pseudomonas subpopulations were represented on the nutrient-poor media. The NAA 1:100 medium, containing ca. 15 mg......We designed five Pseudomonas-selective soil extract NAA media containing the selective properties of trimethoprim and sodium lauroyl sarcosine and 0 to 100% of the amount of Casamino Acids used in the classical Pseudomonas-selective Gould's S1 medium. All of the isolates were confirmed...

  13. The influence of substrate on siderophore production by fish spoilage bacteria

    DEFF Research Database (Denmark)

    Gram, Lone

    1996-01-01

    Siderophore production of fish spoilage bacteria (5 isolates of Shewanella putrefaciens and 5 of Pseudomonas sp.) was determined in fish extract, Tris-succinate medium, a liquid medium of the Chrome-Azurol-S (CAS) agar and in M9 medium supplemented with glucose and casamino acids (M9GC). One Pseu...

  14. Laboratory Aspects of Biological Warfare Agents

    Science.gov (United States)

    2016-01-01

    in cell-free media. The medium, acidified citrate cysteine medium [ACCM; citric acid /sodium citrate, L-cysteine, neopeptone, casamino acids ...Capable of normal completion of life cycle . Viral coat protein - A protein present in the layer surrounding the nucleic acid core of a virus...19 Nucleic Acid

  15. Fuel alcohol production: effects of free amino nitrogen on fermentation of very-high-gravity wheat mashes.

    OpenAIRE

    Thomas, K C; Ingledew, W. M.

    1990-01-01

    Although wheat mashes contain only growth-limiting amounts of free amino nitrogen, fermentations by active dry yeast (Saccharomyces cerevisiae) were completed (all fermentable sugars consumed) in 8 days at 20 degrees C even when the mash contained 35 g of dissolved solids per 100 ml. Supplementing wheat mashes with yeast extract, Casamino Acids, or a single amino acid such as glutamic acid stimulated growth of the yeast and reduced the fermentation time. With 0.9% yeast extract as the supplem...

  16. Bactericidal effect of hydrolysable and condensed tannin extracts on Campylobacter jejuni in vitro.

    Science.gov (United States)

    Anderson, Robin C; Vodovnik, Maša; Min, Byeng R; Pinchak, William E; Krueger, Nathan A; Harvey, Roger B; Nisbet, David J

    2012-07-01

    Strategies are sought to reduce intestinal colonisation of food-producing animals by Campylobacter jejuni, a leading bacterial cause of human foodborne illness worldwide. Presently, we tested the antimicrobial activity of hydrolysable-rich blackberry, cranberry and chestnut tannin extracts and condensed tannin-rich mimosa, quebracho and sorghum tannins (each at 100 mg/mL) against C. jejuni via disc diffusion assay in the presence of supplemental casamino acids. We found that when compared to non-tannin-treated controls, all tested tannins inhibited the growth of C. jejuni and that inhibition by the condensed tannin-rich mimosa and quebracho extracts was mitigated in nutrient-limited medium supplemented with casamino acids. When tested in broth culture, both chestnut and mimosa extracts inhibited growth of C. jejuni and this inhibition was much greater in nutrient-limited than in full-strength medium. Consistent with observations from the disc diffusion assay, the inhibitory activity of the condensed tannin-rich mimosa extracts but not the hydrolysable tannin-rich chestnut extracts was mitigated by casamino acid supplementation to the nutrient-limited medium, likely because the added amino acids saturated the binding potential of the condensed tannins. These results demonstrate the antimicrobial activity of various hydrolysable and condensed tannin-rich extracts against C. jejuni and reveal that condensed tannins may be less efficient than hydrolysable tannins in controlling C. jejuni in gut environments containing high concentrations of amino acids and soluble proteins.

  17. Global expression profiling of Bacillus subtilis cells during industrial-close fed-batch fermentations with different nitrogen sources.

    Science.gov (United States)

    Jürgen, Britta; Tobisch, Steffen; Wümpelmann, Mogens; Gördes, Dirk; Koch, Andreas; Thurow, Kerstin; Albrecht, Dirk; Hecker, Michael; Schweder, Thomas

    2005-11-05

    A detailed gene expression analysis of industrial-close Bacillus subtilis fed-batch fermentation processes with casamino acids as the only nitrogen source and with a reduced casamino acid concentration but supplemented by ammonia was carried out. Although glutamine and arginine are supposed to be the preferred nitrogen sources of B. subtilis, we demonstrate that a combined feeding of ammonia and casamino acids supports cell growth under fed-batch fermentation conditions. The transcriptome and proteome analyses revealed that the additional feeding of ammonia in combination with a reduced amino acid concentration results in a significantly lower expression level of the glnAR or tnrA genes, coding for proteins, which are mainly involved in the nitrogen metabolism of B. subtilis. However, the mRNA levels of the genes of the ilvBHC-leuABD and hom-thrCB operons were significantly increased, indicating a valine, leucine, isoleucine, and threonine limitation under these fermentation conditions. In contrast, during the fermentation with casamino acids as the only nitrogen source, several genes, which play a crucial role in nitrogen metabolism of B. subtilis (e.g., glnAR, nasCDE, nrgAB, and ureABC), were up-regulated, indicating a nitrogen limitation under these conditions. Furthermore, increased expression of genes, which are involved in motility and chemotaxis (e.g., hag, fliT) and in acetoin metabolism (e.g., acoABCL), was determined during the fermentation with the mixed nitrogen source of casamino acids and ammonia, indicating a carbon limitation under these fermentation conditions. Under high cell density and slow growth rate conditions a weak up-regulation of autolysis genes could be observed as well as the induction of a number of genes involved in motility, chemotaxis and general stress response. Results of this study allowed the selection of marker genes, which could be used for the monitoring of B. subtilis fermentation processes. The data suggest for example aco

  18. Fed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis

    Directory of Open Access Journals (Sweden)

    Lalk Michael

    2010-09-01

    Full Text Available Abstract Background Pseudoalteromonas haloplanktis is a cold-adapted γ-proteobacterium isolated from Antarctic sea ice. It is characterized by remarkably high growth rates at low temperatures. P. haloplanktis is one of the model organisms of cold-adapted bacteria and has been suggested as an alternative host for the soluble overproduction of heterologous proteins which tend to form inclusion bodies in established expression hosts. Despite the progress in establishing P. haloplanktis as an alternative expression host the cell densities obtained with this organism, which is unable to use glucose as a carbon source, are still low. Here we present the first fed-batch cultivation strategy for this auspicious alternative expression host. Results The key for the fed-batch cultivation of P. haloplanktis was the replacement of peptone by casamino acids, which have a much higher solubility and allow a better growth control. In contrast to the peptone medium, on which P. haloplanktis showed different growth phases, on a casamino acids-containing, phosphate-buffered medium P. haloplanktis grew exponentially with a constant growth rate until the stationary phase. A fed-batch process was established by feeding of casamino acids with a constant rate resulting in a cell dry weight of about 11 g l-1 (OD540 = 28 which is a twofold increase of the highest densities which have been obtained with P. haloplanktis so far and an eightfold increase of the density obtained in standard shake flask cultures. The cell density was limited in the fed-batch cultivation by the relatively low solubility of casamino acids (about 100 g l-1, which was proven by pulse addition of casamino acid powder which increased the cell density to about 20 g l-1 (OD540 = 55. Conclusion The growth of P. haloplanktis to higher cell densities on complex medium is possible. A first fed-batch fermentation strategy could be established which is feasible to be used in lab-scale or for industrial

  19. Growth of the fish pathogen Renibacterium salmoninarum on different media.

    Science.gov (United States)

    Bandín, I; Santos, Y; Barja, J I; Toranzo, A E

    1996-09-01

    In the present study, the ability of a group of Renibacterium salmoninarum strains to grow in the presence or absence of the amino acid cysteine and other mineral and organic sources of sulfur and nitrogen has been evaluated. Most of the isolates tested were able to grow on a mineral media supplemented with L-cysteine-HCl or other organic compounds, such as the vitamin thiamine and a casein hydrolysate (Bacto Casamino Acids, Difco). Bacterial growth was also recorded on commercial and specific media not supplemented with L-cysteine-HCl, or in which this amino acid was replaced by the compounds cited above.

  20. Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L.

    OpenAIRE

    2001-01-01

    International audience; Standardized explants composed of hypocotyl and root-tip were prepared from embryonic structures obtained from one embryogenic line of Quercus robur L. maintained by regular transfer onto a solidified reference medium composed of the MS mineral solutions, glucose (0.12 M), casamino acid (0.1% ), and NAA (10.74 $\\mu$M). The regeneration capacity from these explants were tested on the reference medium and on 2 alternative media in which the NAA (10.74 $\\mu$M) was omitted...

  1. Growth of Campylobacter incubated aerobically in fumarate-pyruvate media or media supplemented with dairy, meat, or soy extracts and peptones.

    Science.gov (United States)

    Hinton, Arthur

    2016-09-01

    The ability of Campylobacter to grow aerobically in media supplemented with fumarate-pyruvate or with dairy, meat, or soy extracts or peptones was examined. Optical densities (OD) of Campylobacter cultured in basal media, media supplemented with fumarate-pyruvate or with 1.0, 2.5, 5.0, or 7.5% beef extract was measured. Growth was also compared in media supplemented with other extracts or peptones. Finally, cfu/mL of Campylobacter recovered from basal media or media supplemented with fumarate-pyruvate, casamino acids, beef extract, soytone, or beef extract and soytone was determined. Results indicated that OD of cultures grown in media supplemented with fumarate-pyruvate or with 5.0 or 7.5% beef extract were higher than OD of isolates grown in basal media or media supplemented with lower concentrations of beef extract. Highest OD were produced by isolates grown in media supplemented with beef extract, peptone from meat, polypeptone, proteose peptone, or soytone. Also, more cfu/mL were recovered from media with fumarate-pyruvate, beef extract, soytone, or beef extract-soytone than from basal media or media with casamino acids. Findings indicate that media supplemented with organic acids, vitamins, and minerals and media supplemented with extracts or peptones containing these metabolites can support aerobic growth of Campylobacter. Published by Elsevier Ltd.

  2. Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources.

    Science.gov (United States)

    Domin, Nicole; Wilson, Duncan; Brock, Matthias

    2009-12-01

    Propionyl-CoA is an inhibitor of both primary and secondary metabolism in Aspergillus species and a functional methylcitrate cycle is essential for the efficient removal of this potentially toxic metabolite. Although the genomes of most sequenced fungal species appear to contain genes coding for enzymes of the methylcitrate cycle, experimental confirmation of pathway activity in filamentous fungi has only been provided for Aspergillus nidulans and Aspergillus fumigatus. In this study we demonstrate that pathogenic Fusarium species also possess a functional methylcitrate cycle. Fusarium solani appears highly adapted to saprophytic growth as it utilized propionate with high efficiency, whereas Fusarium verticillioides grew poorly on this carbon source. In order to elucidate the mechanisms of propionyl-CoA detoxification, we first identified the genes coding for methylcitrate synthase from both species. Despite sharing 96 % amino acid sequence identity, analysis of the two purified enzymes demonstrated that their biochemical properties differed in several respects. Both methylcitrate synthases exhibited low K(m) values for propionyl-CoA, but that of F. verticillioides displayed significantly higher citrate synthase activity and greater thermal stability. Activity determinations from cell-free extracts of F. solani revealed a strong methylcitrate synthase activity during growth on propionate and to a lesser extent on Casamino acids, whereas activity by F. verticillioides was highest on Casamino acids. Further phenotypic analysis confirmed that these biochemical differences were reflected in the different growth behaviour of the two species on propionyl-CoA-generating carbon sources.

  3. Enhanced bio-decolorization of 1-amino-4-bromoanthraquinone-2-sulfonic acid by Sphingomonas xenophaga with nutrient amendment

    Institute of Scientific and Technical Information of China (English)

    Hong Lu; Xiaofan Guan; Jing Wang; Jiti Zhou; Haikun Zhang

    2015-01-01

    Bacterial decolorization of anthraquinone dye intermediates is a slow process under aerobic conditions.To speed up the process,in the present study,effects of various nutrients on 1-amino-4-bromoanthraquinone-2-sulfonic acid (ABAS) decolorization by Sphingomonas xenophaga QYY were investigated.The results showed that peptone,yeast extract and casamino acid amendments promoted ABAS bio-decolorization.In particular,the addition of peptone and casamino acids could improve the decolorization activity of strain QYY.Further experiments showed that L-proline had a more significant accelerating effect on ABAS decolorization compared with other amino acids.L-Proline not only supported cell growth,but also significantly increased the decolorization activity of strain QYY.Membrane proteins of strain QYY exhibited ABAS decolorization activities in the presence of L-proline or reduced nicotinamide adenine dinucleotide,while this behavior was not observed in the presence of other amino acids.Moreover,the positive correlation between L-proline concentration and the decolorization activity of membrane proteins was observed,indicating that L-proline plays an important role in ABAS decolorization.The above findings provide us not only a novel insight into bacterial ABAS decolorization,but also an L-proline-supplemented bioaugmentation strategy for enhancing ABAS bio-decolorization.

  4. Nutrients interaction investigation to improve Monascus purpureus FTC5391 growth rate using Response Surface Methodology and Artificial Neural Network

    Directory of Open Access Journals (Sweden)

    Mohamad, R.

    2013-01-01

    Full Text Available Aims: Two vital factors, certain environmental conditions and nutrients as a source of energy are entailed for successful growth and reproduction of microorganisms. Manipulation of nutritional requirement is the simplest and most effectual strategy to stimulate and enhance the activity of microorganisms. Methodology and Results: In this study, response surface methodology (RSM and artificial neural network (ANN were employed to optimize the carbon and nitrogen sources in order to improve growth rate of Monascus purpureus FTC5391,a new local isolate. The best models for optimization of growth rate were a multilayer full feed-forward incremental back propagation network, and a modified response surface model using backward elimination. The optimum condition for cell mass production was: sucrose 2.5%, yeast extract 0.045%, casamino acid 0.275%, sodium nitrate 0.48%, potato starch 0.045%, dextrose 1%, potassium nitrate 0.57%. The experimental cell mass production using this optimal condition was 21 mg/plate/12days, which was 2.2-fold higher than the standard condition (sucrose 5%, yeast extract 0.15%, casamino acid 0.25%, sodium nitrate 0.3%, potato starch 0.2%, dextrose 1%, potassium nitrate 0.3%. Conclusion, significance and impact of study: The results of RSM and ANN showed that all carbon and nitrogen sources tested had significant effect on growth rate (P-value < 0.05. In addition the use of RSM and ANN alongside each other provided a proper growth prediction model.

  5. Ammonia-Hyperproducing Bacteria from New Zealand Ruminants

    Science.gov (United States)

    Attwood, Graeme T.; Klieve, Athol V.; Ouwerkerk, Diane; Patel, Bharat K. C.

    1998-01-01

    Pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (HAP) bacteria in roll tubes containing a medium in which tryptone and Casamino Acids were the sole nitrogen and energy sources. Colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. A total of 14 morphologically distinct colonies were purified and studied further. Restriction fragment length polymorphisms of 16S rRNA genes indicated that all isolates differed from the previously described HAP bacteria, Clostridium aminophilum, Clostridium sticklandii, and Peptostreptococcus anaerobius. Carbon source utilization experiments showed that five isolates (C2, D1, D4, D5, and S1) were unable to use any, or very few, of the carbon sources tested. Biochemical tests and phylogenetic analyses of 16S ribosomal DNA sequences indicated that all isolates were monensin sensitive; that D1 and S1 belonged to the genus Peptostreptococcus, that D4 and D5 belonged to the family Bacteroidaceae, where D4 was similar to Fusobacterium necrophorum; and that C2 was most similar to an unidentified species from the genus Eubacterium. Growth on liquid medium containing tryptone and Casamino Acids as the sole nitrogen and energy source showed that D1, D4, and S1 grew rapidly (specific growth rates of 0.40, 0.35, and 0.29 h−1, respectively), while C2 and D5 were slow growers (0.25 and 0.10 h−1, respectively). Ammonia production rates were highest in D1 and D4, which produced 945.5 and 748.3 nmol/min per mg of protein, respectively. Tests of individual nitrogen sources indicated that D1 and D4 grew best on tryptone, S1 grew equally well on Casamino Acids or tryptone, and C2 and D5 grew poorly on all nitrogen sources. The intact proteins casein and gelatin did not support significant growth of any of the isolates. These isolates extend the diversity of known HAP rumen bacteria and indicate the presence of

  6. A Study of Low Level Laser Retinal Damage.

    Science.gov (United States)

    1983-03-15

    Wavelength and Solution Concentration" Biochemica et Biophysica Acta, 102, 459, (1965) 52) Kosower , E.M. &#34The Effect of Solvent on Spectra I: A New...Empirical Measure of Solvent Polarity: Z-Values" J. Am Chem Soc 80, 3253, 1958. 53) Kosower , E.M. &#34The Effect of Solvent on Spectra II: Correlation of

  7. Engineering Applications of Neural Computing: A State-of-the-Art Survey

    Science.gov (United States)

    1991-05-01

    Himmelblau , D. M., &#34Introducing Efficient Second Order Effects into Backpropagation Learning," Proceedings of the International Joint Conference on...Conference on Neural Networks, 1-569. 10. Hoskins, J. C., Kaliyur, K. M., Himmelblau , D. M., &#34Insipient Fault Detection and Diagnosis Using Artificial

  8. Flow Control Over Sharp-Edged Wings

    Science.gov (United States)

    2007-07-01

    Sharp Leading Edge," Paper No. AIAA-2001-0121. 12Washburn, A. E., and Amitay, M., &#34Active Flow Control on the Stingray UAV: Physical Mechanisms," 4 2...A. E., and Amitay, M., &#34Active Flow Control on the Stingray UAV: Physical Mechanisms," 42 Aerospace Sciences Meeting & Exhibit," Paper No. AIAA-2004

  9. [Optimization on the production of analgesic peptide from Buthus martensii Karsch in Pichia pastoris].

    Science.gov (United States)

    Yang, Jin-ling; He, Hui-xia; Zhu, Hui-xin; Cheng, Ke-di; Zhu, Ping

    2009-01-01

    The technology of liquid fermentation for producing the recombinant analgesic peptide BmK AngM1 from Buthus martensii Karsch in Pichia pastoris was studied by single-factor and orthogonal test. The results showed that the optimal culture conditions were as follows: 1.2% methanol, 0.6% casamino acids, initial pH 6.0, and three times of basal inoculation volume. Under the above culture conditions, the expression level of recombinant BmK AngM1 in Pichia pastoris was above 500 mg x L(-1), which was more than three times of the control. The study has laid a foundation for the large-scale preparation of BmK AngM1 to meet the needs of theoretical research of BmK AngM1 and development of new medicines.

  10. Heterologous expression of trametes versicolor laccase in pichia pastoris and aspergillus niger

    CSIR Research Space (South Africa)

    Bohlin, C

    2006-09-01

    Full Text Available concentration of 1 × 106 spores/mL. The inoculum was generated by resuspending spores in a sterile 0.9% NaCl solution. The spore suspen- sion was stored at 4°C until use. The cultures were incubated in a volume of 100 mL at 30°C with shaking (200 rpm....1% casamino acids (Difco, BD, Franklin Lakes, NJ), 2% MgSO4⋅7H2O, 1X trace element solution, and 1X AspA with nitrate by inoculation with spores to a concentration of 1 × 106 spores/mL (experimental series 1). The cultures were then grown for 48, 72, or 144...

  11. The development of haploid embryoids from anther cultures of Atropa belladonna L.

    Science.gov (United States)

    Rashid, A; Street, H E

    1973-09-01

    Development of haploid embryoids from the microspores of Atropa belladonna occurs with relatively high frequency when anthers are excised from buds in which the petals are shorter than the sepals (at this stage microspores are predominantly uninucleate) and cultured on a medium containing iron as the ferric salt of ethylenediamine-di-O-hydroxyphenylacetic acid (FeEDDHA). Additions of combinations of kinetin, auxin and casamino-acids to the culture medium induce callusing in both haploid and diploid tissues, lead to the origin of embryoids from somatic tissues of the anther and should be avoided. Simple techniques for the maintenance of haploid clones are described.Stages in early embryogenesis in the pollen grains have been observed and these indicate that embryogenesis is most frequently initiated by an equal division in the uninucleate spore. The frequency of grains showing embryoid formation is very low and it is estimated that plantlets are formed from up to 50% of these grains.

  12. Expression of NEP1 by Fusarium oxysporum f. sp. erythroxyli After Gene Replacement and Overexpression Using Polyethylene Glycol-Mediated Transformation.

    Science.gov (United States)

    Bailey, B A; Apel-Birkhold, Patricia C; Luster, Douglas G

    2002-08-01

    ABSTRACT The necrosis inducing extracellular protein Nep1 is produced by Fusarium oxysporum f. sp. erythroxyli in liquid culture. NEP1, the Nep1 protein structural gene, was disrupted in F. oxysporum f. sp. erythroxyli isolate EN-4 by gene replacement using polyethylene glycol (PEG)-mediated transformation. NEP1 disruption was verified by polymerase chain reaction (PCR), Southern blot, and northern blot analysis. NEP1-disrupted transformants failed to produce Nep1 in liquid culture. NEP1 disruption did not affect the pathogenicity of isolate EN-4 toward Erythroxylum coca. Transformation of isolate EN-4 with construct pPB-FO11-45 carrying NEP1 between the trpC promoter and terminator resulted in increased production of Nep1 in potato dextrose broth plus 1% casamino acids or Czapek-Dox broth plus 1% casamino acids but not in potato dextrose broth alone. Transformation of EN-4 with construct pPB-FO11-45 was verified by PCR and Southern blot analysis. Overexpression of NEP1 was confirmed by northern blot and Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. NEP1-overexpressing transformant 15 produced 64 to 128 times as much Nep1 as EN-4 wild type when grown in shake cultures. Transformants overexpressing Nep1 in liquid culture were no more or less pathogenic toward E. coca than wild-type isolates. Nep1 was not detected in E. coca seedlings infected with NEP1-overexpressing transformants or with EN-4 wild type. In large-scale fermentations of NEP1-overexpressing transformant 15, the amount of secreted protein including Nep1 was 15.1 times that of the wild-type EN-4, providing a ready source of Nep1 for future study.

  13. Enhanced production of histamine dehydrogenase by Natrinema gari BCC 24369 in a non-sterile condition.

    Science.gov (United States)

    Chaikaew, Siriporn; Powtongsook, Sorawit; Boonpayung, Somphop; Benjakul, Soottawat; Visessanguan, Wonnop

    2015-01-01

    The production of histamine dehydrogenase (HADH) by Natrinema gari BCC 24369, a halophilic archeaon isolated from fish sauce, was optimized and scaled up under a non-sterile condition. Through statistical design by Plackett-Burman design (PBD), casamino acid, NaCl, MgSO4·7H2O and FeCl2·4H2O were identified as the significant medium compositions influencing HADH production. Central composite design (CCD) was employed to identify the optimal values of individual composition yielding the maximum HADH production. The analysis indicated that the optimal medium was composed of 15 g/l casamino acid, 75 g/l MgSO4·7H2O, 273 g/l NaCl, 2.5 mg/l FeCl2·4H2O, 10 g/l yeast extract, 5 g/l sodium glutamate and 5 g/l KCl. Based on the one-factor-at-a-time (OFAT) method, the optimum initial pH of the culture medium and the incubation temperature for HADH production were 7.5 and 37 °C, respectively. The production of HADH under optimal conditions was 2.2-fold higher than that under un-optimized conditions. Owing to the halophilic nature of Nnm. gari BCC 24369, a more economical and eco-friendlier HADH production was developed under a completely non-sterile condition. In a 16-l batch cultivation of Nnm. gari BCC 24369, HADH productivity under a non-sterile condition (858 ± 12 U/g cell biomass) was comparable to that under a sterile condition (878 ± 15 U/g cell biomass). These results demonstrate the feasibility and simplicity of HADH production using Nnm. gari BCC 24369 under a non-sterile condition without compromising enzyme yield and any changes in Km value.

  14. The Prevalence and Importance of Epithelial Plasticity in Metastatic Prostate Cancer

    Science.gov (United States)

    2014-10-01

    34Cancer#13,ඩV110.൛" De"Gasperi,"R.,"Rocher,"A."B.,"Sosa,"M.&#34A.,"Wearne,"S."L.," Perez ,"G."M.,&#34Friedrich,"V."L.,"Jr.,"Hof,"P."R.,"and൜" Elder,"G."A...34 Nieto ,"M.&#34A."(2012)."Metastatic"colonization"requires"the"repression"of"the"epithelialV36" mesenchymal"transition"inducer"Prrx1."Cancer"Cell#22,螕V724

  15. Hispanics and the Military: A Reference Data Base.

    Science.gov (United States)

    1986-02-01

    the cases he describes are those reported by Army doctors in Puerto Rican units. Laosa, Luis M. &#34Bilingualism in Three United States’ Hispanic...Mexican- ’ Americans and Cubans. Laosa, Luis M. &#34Cognitive Styles and Learning Strategies Research." Journal of Teacher Education 28 (No. 3, 1977):26-30...US Bureau of the Census, 1976). It is a statistical analysis that looks at various sources of wage loss for Hispanic subgroups. JI. Rincon , Edward T

  16. Hispanics and the Military: A Selected Annotated Bibliography.

    Science.gov (United States)

    1985-08-01

    Graduates. Rincon , E. T., "Aptitude Testing, Higher Education, and Minority Groups: A Review of the Issues and Research." Rossman, J. E., Private... Luis M. &#34Cognitive Styles and Learning Strategies Research." Journal of Teacher Education 28 (No. 3, 1977):26-30. This is useful as a background...children. Laosa, Luis M. &#34Bilingualism in Three United States Hispanic Groups: Contextual Use of Language by Children and Adults in Their Families

  17. Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters.

    Science.gov (United States)

    Ogg, Christopher D; Patel, Bharat K C

    2010-06-01

    A strictly anaerobic, thermophilic bacterium, designated strain AeB(T), was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeB(T) were slightly curved rods (2.5-6.0x1.0 mum) that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in tryptone-yeast extract-Casamino acids medium at 50 degrees C (range 37-55 degrees C) and pH 7 (range pH 5-9). Strain AeB(T) grew poorly on yeast extract (0.2 %) and tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeB(T) increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO2 and H2. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4+/-1 mol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeB(T) is a member of the family Clostridiaceae, class Clostridia, phylum 'Firmicutes', and is positioned approximately equidistantly between the genera Sarcina, Anaerobacter, Caloramator and Clostridium (16S rRNA gene similarity values of 87.8-90.9 %). On the basis of 16S rRNA gene

  18. Dextran sodium sulfate enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe.

    Science.gov (United States)

    Mukaiyama, Hiroyuki; Giga-Hama, Yuko; Tohda, Hideki; Takegawa, Kaoru

    2009-11-01

    The effect of medium supplementation on heterologous production of human serum transferrin (hTF) in the fission yeast Schizosaccharomyces pombe has been investigated. The productivity of recombinant hTF was low in wild-type S. pombe cells. To overcome this impediment, culture media supplements were screened for their ability to improve secretion of hTF. Casamino acids (CAA), which have been reported to increase heterologous protein productivity in Pichia pastoris, improved the secretion hTF by more than fourfold. An anion surfactant deoxycholate or polyethylene glycol also improved the secretion hTF. Interestingly, dextran sodium sulfate (DSS), a poly-anion surfactant, was found to enhance production of secreted hTF better than any other supplement tested. Addition of DSS in the presence of 2% CAA exhibited a synergistic effect on increasing hTF secretion, resulting in an increase of about sevenfold relative to conventional conditions. Cell growth was not found to be affected by the addition of DSS or CAA. DSS may act as a surfactant and may also facilitate the anchoring of liposomes, and these properties may contribute to efficient secretion or exocytosis through the plasma membrane.

  19. ISOLATION AND CHARACTERIZATION OF A NOVEL BENZOATE- UTILIZING Serratia marcescens

    Directory of Open Access Journals (Sweden)

    ANTONIUS SUWANTO

    2003-01-01

    Full Text Available A new benzoate-utilizing strain, Serratia marcescens DS-8, isolated from the environment was characterized. The strain was enterobacilli, Gram negative, mesophilic, non ha lophilic, and aerobic bacterium that showed motile ovale-rod shaped cells. The isolate produced extracellular chitinase, protease, and prodigiosin (a red pigment pr oduced by several Serratia strains yielding bright red or pink colonies. A physiological assay using Microbact* test showed that the strain was closely related to Klebsiella ozaenae (49.85% and Serratia liquefaciens (24.42%, respectively. However, 16S rRNA sequence analysis indicated that the strain was closely related to S. marcescens DSM 30121 with similarity level of 98%. DS-8 strain was able to synthesize its own vitamins. Optimum growth in benzoate was obtained at pH between 7-8.5 and NaCl concentration of 1- 1.5% (w/v. The isolate could grow in benzoate-containing medium up to 10 mM. Other carbon sources that could support the growth of DS-8 were casamino acid, glutamate, glucose, acetate, potato star ch, and ethanol.

  20. Growth medium-dependent glycine incorporation into the peptidoglycan of Caulobacter crescentus.

    Directory of Open Access Journals (Sweden)

    Constantin N Takacs

    Full Text Available The peptidoglycan (PG is a macromolecular component of the bacterial cell wall that maintains the shape and integrity of the cell. The PG of Caulobacter crescentus, unlike that of many other Gram-negative bacteria, has repeatedly been shown to contain significant amounts of glycine. This compositional peculiarity has been deemed an intrinsic characteristic of this species. By performing a comprehensive qualitative and quantitative analysis of the C. crescentus PG by high-performance liquid chromatography (HPLC and mass spectrometry (MS, we show here that glycine incorporation into the C. crescentus PG depends on the presence of exogenous glycine in the growth medium. High levels of glycine were detected at the fifth position of the peptide side chains of PG isolated from C. crescentus cells grown in the complex laboratory medium PYE or in defined medium (M2G supplemented with casamino acids or glycine alone. In contrast, glycine incorporation was undetectable when cells were grown in M2G medium lacking glycine. Remarkably, glycine incorporation into C. crescentus peptidoglycan occurred even in the presence of low millimolar to sub-millimolar concentrations of free glycine. High glycine content in the PG had no obvious effects on growth rates, mode of PG incorporation or cell morphology. Hence, the C. crescentus PG is able to retain its physiological functions in cell growth and morphogenesis despite significant alterations in its composition, in what we deem to be unprecedented plasticity.

  1. Growth medium-dependent glycine incorporation into the peptidoglycan of Caulobacter crescentus.

    Science.gov (United States)

    Takacs, Constantin N; Hocking, Jason; Cabeen, Matthew T; Bui, Nhat Khai; Poggio, Sebastian; Vollmer, Waldemar; Jacobs-Wagner, Christine

    2013-01-01

    The peptidoglycan (PG) is a macromolecular component of the bacterial cell wall that maintains the shape and integrity of the cell. The PG of Caulobacter crescentus, unlike that of many other Gram-negative bacteria, has repeatedly been shown to contain significant amounts of glycine. This compositional peculiarity has been deemed an intrinsic characteristic of this species. By performing a comprehensive qualitative and quantitative analysis of the C. crescentus PG by high-performance liquid chromatography (HPLC) and mass spectrometry (MS), we show here that glycine incorporation into the C. crescentus PG depends on the presence of exogenous glycine in the growth medium. High levels of glycine were detected at the fifth position of the peptide side chains of PG isolated from C. crescentus cells grown in the complex laboratory medium PYE or in defined medium (M2G) supplemented with casamino acids or glycine alone. In contrast, glycine incorporation was undetectable when cells were grown in M2G medium lacking glycine. Remarkably, glycine incorporation into C. crescentus peptidoglycan occurred even in the presence of low millimolar to sub-millimolar concentrations of free glycine. High glycine content in the PG had no obvious effects on growth rates, mode of PG incorporation or cell morphology. Hence, the C. crescentus PG is able to retain its physiological functions in cell growth and morphogenesis despite significant alterations in its composition, in what we deem to be unprecedented plasticity.

  2. Diversities in virulence, antifungal activity, pigmentation and DNA fingerprint among strains of Burkholderia glumae.

    Science.gov (United States)

    Karki, Hari S; Shrestha, Bishnu K; Han, Jae Woo; Groth, Donald E; Barphagha, Inderjit K; Rush, Milton C; Melanson, Rebecca A; Kim, Beom Seok; Ham, Jong Hyun

    2012-01-01

    Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly correlated to virulence on onion bulb scales, suggesting that onion bulb can be a convenient alternative host system to efficiently determine the virulence of B. glumae strains. Production of toxoflavin, the phytotoxin that functions as a major virulence factor, was closely associated with the virulence phenotypes of B. glumae strains in rice. Some strains of B. glumae showed various levels of antifungal activity against Rhizoctonia solani, the causal agent of sheath blight, and pigmentation phenotypes on casamino acid-peptone-glucose (CPG) agar plates regardless of their virulence traits. Purple and yellow-green pigments were partially purified from a pigmenting strain of B. glumae, 411gr-6, and the purple pigment fraction showed a strong antifungal activity against Collectotrichum orbiculare. Genetic variations were detected among the B. glumae strains from DNA fingerprinting analyses by repetitive element sequence-based PCR (rep-PCR) for BOX-A1R-based repetitive extragenic palindromic (BOX) or enterobacterial repetitive intergenic consensus (ERIC) sequences of bacteria; and close genetic relatedness among virulent but pigment-deficient strains were revealed by clustering analyses of DNA fingerprints from BOX-and ERIC-PCR.

  3. A conserved two-component regulatory system, PidS/PidR, globally regulates pigmentation and virulence-related phenotypes of Burkholderia glumae.

    Science.gov (United States)

    Karki, Hari Sharan; Barphagha, Inderjit Kaur; Ham, Jong Hyun

    2012-09-01

    Burkholderia glumae is a rice pathogenic bacterium that causes bacterial panicle blight. Some strains of this pathogen produce dark brown pigments when grown on casamino-acid peptone glucose (CPG) agar medium. A pigment-positive and highly virulent strain of B. glumae, 411gr-6, was randomly mutagenized with mini-Tn5gus, and the resulting mini-Tn5gus derivatives showing altered pigmentation phenotypes were screened on CPG agar plates to identify the genetic elements governing the pigmentation of B. glumae. In this study, a novel two-component regulatory system (TCRS) composed of the PidS sensor histidine kinase and the PidR response regulator was identified as an essential regulatory factor for pigmentation. Notably, the PidS/PidR TCRS was also required for the elicitation of the hypersensitive response on tobacco leaves, indicating the dependence of the hypersensitive response and pathogenicity (Hrp) type III secretion system of B. glumae on this regulatory factor. In addition, B. glumae mutants defective in the PidS/PidR TCRS showed less production of the phytotoxin, toxoflavin, and less virulence on rice panicles and onion bulbs relative to the parental strain, 411gr-6. The presence of highly homologous PidS and PidR orthologues in other Burkholderia species suggests that PidS/PidR-family TCRSs may exert the same or similar functions in different Burkholderia species, including both plant and animal pathogens.

  4. Diversities in virulence, antifungal activity, pigmentation and DNA fingerprint among strains of Burkholderia glumae.

    Directory of Open Access Journals (Sweden)

    Hari S Karki

    Full Text Available Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly correlated to virulence on onion bulb scales, suggesting that onion bulb can be a convenient alternative host system to efficiently determine the virulence of B. glumae strains. Production of toxoflavin, the phytotoxin that functions as a major virulence factor, was closely associated with the virulence phenotypes of B. glumae strains in rice. Some strains of B. glumae showed various levels of antifungal activity against Rhizoctonia solani, the causal agent of sheath blight, and pigmentation phenotypes on casamino acid-peptone-glucose (CPG agar plates regardless of their virulence traits. Purple and yellow-green pigments were partially purified from a pigmenting strain of B. glumae, 411gr-6, and the purple pigment fraction showed a strong antifungal activity against Collectotrichum orbiculare. Genetic variations were detected among the B. glumae strains from DNA fingerprinting analyses by repetitive element sequence-based PCR (rep-PCR for BOX-A1R-based repetitive extragenic palindromic (BOX or enterobacterial repetitive intergenic consensus (ERIC sequences of bacteria; and close genetic relatedness among virulent but pigment-deficient strains were revealed by clustering analyses of DNA fingerprints from BOX-and ERIC-PCR.

  5. Gelidivirgula Patagoniensis Gen. Nov., Sp. Nov., A Novel Psychrotolerant, Sporeforming Anaerobe Isolated from Magellanic Penguin Guano in Patagonia, Chile

    Science.gov (United States)

    Pikuta, Elena V.; Hoover, Richard B.; Marsic, Damien; Whitman, William B.; Tang, Jane; Krader, Paul

    2003-01-01

    A novel obligately anaerobic, psychrotrophic bacterium, strain PPP2(sup T), was isolated from guano of the Magellanic penguin (Spheniscus magellanicus) in Patagonia, Chile. The Gram-positive, sporeforming, straight rods with sizes 0.6-0.9 x 3.0-5.0 microns, are motile by peritrichous flagella. Growth was observed to occur within the pH range 6.0-9.5 (optimum pH x), and temperature range 2-28 C (optimum 20 C). The novel isolate does not require NaCl for growth, but is halotolerant and growth was observed between 0 and 7 % NaCl (w/v) with optimum at 0.5 % (w/v). The new isolate is a catalase negative chemoorganohetherotroph with fermentative metabolism and uses as substrates: peptone, Bacto-tryptone, Casamino acids, and yeast extract. The major metabolic products are: acetate, butyrate, ethanol, and hydrogen is a minor gas product.. Strain PPP2 was sensitive to ampicillin, tetracycline, chloramphenicol, rifampin, kanamycin, and gentamycin. The G+C content of the DNA is 43.6 mol%. On the basis of 16S rDNA gene sequences and phenotypic characteristics, it is proposed that the strain PPP2(sup T) (= ATCC BAA-755(sup T) = JSM ...(sup T)) is assigned to the new genus Gelidivirgula gen. nov., as a representative of the new species, Gelidivirgula patagonensis sp. nov.

  6. Improving the culturability of freshwater bacteria using FW70, a low-nutrient solid medium amended with sodium pyruvate.

    Science.gov (United States)

    Imazaki, Iori; Kobori, Youichi

    2010-04-01

    Bacterial culture based on the use of plate media is an effective method for investigating bacterial populations in the environment. To improve the culturability of bacteria from freshwater lakes, we developed a new medium, FW70, which contains sodium pyruvate, casamino acids, and lake water and is solidified using gellan gum. To test the importance of these components, we prepared a series of media in which one or more of the components was absent. Water was sampled 31 times from 3 Japanese lakes and was passed through a membrane filter (pore size = 0.45 microm) to remove fast-growing microbes before the water was spread onto the plates. In most cases, significantly larger numbers of bacterial colonies were detected on FW70 than on other media. Furthermore, to test the practicality of FW70, we compared it with standard nutrient agar and R2A agar. In all cases, the culturability was significantly greater on FW70 than on standard nutrient agar or R2A agar. Some isolates recovered by means of FW70 belonged to bacteria that had not previously been classified. These results suggest that FW70 improves the culturability of freshwater bacteria and can be used for the isolation of novel bacteria as a result of the filtration step.

  7. Biosynthesis of pyridoxine in Saccharomyces cerevisiae--origin of the pyridoxine nitrogen atom differs under anaerobic and aerobic conditions.

    Science.gov (United States)

    Ishida, Shiho; Yamada, Kazuko

    2002-12-01

    The amide nitrogen atom of glutamine is incorporated into pyridoxine in four eukaryotes (i.e., Emericella nidulans, Mucor racemosus, Neurospora crassa and Saccharomyces cerevisiae) and two prokaryotes (i.e., Staphylococcus aureus and Bacillus subtilis). However, in the prokaryotes Pseudomonas putida, Enterobacter aerogenes and Escherichia coli, it is the nitrogen atom of glutamate that is incorporated into pyridoxine (J Nutr Sci Vitaminol (2000) 46, 55-57). As these results were from experiments conducted under aerobic conditions, we investigated the biosynthesis of pyridoxine on S. cerevisiae under anaerobic conditions. The results showed that [amide-15N]L-glutamine was not incorporated into pyridoxine, unlike the results for aerobic conditions. The incorporation of [15N]ammonium salts into pyridoxine was not inhibited in the presence of casamino acids and tryptophan. The results showed that the nitrogen atoms of amino acids are not used for the biosynthesis of pyridoxine. The incorporation of 15N into pyridoxine was inhibited in the presence of adenine, but not in that of hypoxanthine. Thus, the nitrogen atom of pyridoxine may be from the amino group attached to the C-6 of adenine.

  8. Amino Acid and Peptide Utilization Profiles of the Fluoroacetate-Degrading Bacterium Synergistetes Strain MFA1 Under Varying Conditions.

    Science.gov (United States)

    Leong, Lex E X; Denman, Stuart E; Hugenholtz, Philip; McSweeney, Christopher S

    2016-02-01

    Synergistetes strain MFA1 is an asaccharolytic ruminal bacterium isolated based on its ability to degrade fluoroacetate, a plant toxin. The amino acid and peptide requirements of the bacterium were investigated under different culturing conditions. The growth of strain MFA1 and its fluoroacetate degradation rate were enhanced by peptide-rich protein hydrolysates (tryptone and yeast extract) compared to casamino acid, an amino acid-rich protein hydrolysate. Complete utilization and preference for arginine, asparagine, glutamate, glycine, and histidine as free amino acids from yeast extract were observed, while the utilization of serine, threonine, and lysine in free form and peptide-bound glutamate was stimulated during growth on fluoroacetate. A predominant peptide in yeast extract preferentially utilized by strain MFA1 was partially characterized by high-liquid performance chromatography-mass spectrometry as a hepta-glutamate oligopeptide. Similar utilization profiles of amino acids were observed between the co-culture of strain MFA1 with Methanobrevibacter smithii without fluoroacetate and pure strain MFA1 culture with fluoroacetate. This suggests that growth of strain MFA1 could be enhanced by a reduction of hydrogen partial pressure as a result of hydrogen removal by a methanogen or reduction of fluoroacetate.

  9. Production of volatile aroma compounds by bacterial strains isolated from different surface-ripened French cheeses.

    Science.gov (United States)

    Deetae, Pawinee; Bonnarme, Pascal; Spinnler, Henry E; Helinck, Sandra

    2007-10-01

    Twelve bacterial strains belonging to eight taxonomic groups: Brevibacterium linens, Microbacterium foliorum, Arthrobacter arilaitensis, Staphylococcus cohnii, Staphylococcus equorum, Brachybacterium sp., Proteus vulgaris and Psychrobacter sp., isolated from different surface-ripened French cheeses, were investigated for their abilities to generate volatile aroma compounds. Out of 104 volatile compounds, 54 volatile compounds (identified using dynamic headspace technique coupled with gas chromatography-mass spectrometry [GC-MS]) appeared to be produced by the different bacteria on a casamino acid medium. Four out of eight species used in this study: B. linens, M. foliorum, P. vulgaris and Psychrobacter sp. showed a high flavouring potential. Among these four bacterial species, P. vulgaris had the greatest capacity to produce not only the widest varieties but also the highest quantities of volatile compounds having low olfactive thresholds such as sulphur compounds. Branched aldehydes, alcohols and esters were produced in large amounts by P. vulgaris and Psychrobacter sp. showing their capacity to breakdown the branched amino acids. This investigation shows that some common but rarely mentioned bacteria present on the surface of ripened cheeses could play a major role in cheese flavour formation and could be used to produce cheese flavours.

  10. Portable hyperspectral fluorescence imaging system for detection of biofilms on stainless steel surfaces

    Science.gov (United States)

    Jun, Won; Lee, Kangjin; Millner, Patricia; Sharma, Manan; Chao, Kuanglin; Kim, Moon S.

    2008-04-01

    A rapid nondestructive technology is needed to detect bacterial contamination on the surfaces of food processing equipment to reduce public health risks. A portable hyperspectral fluorescence imaging system was used to evaluate potential detection of microbial biofilm on stainless steel typically used in the manufacture of food processing equipment. Stainless steel coupons were immersed in bacterium cultures, such as E. coli, Pseudomonas pertucinogena, Erwinia chrysanthemi, and Listeria innocula. Following a 1-week exposure, biofilm formations were assessed using fluorescence imaging. In addition, the effects on biofilm formation from both tryptic soy broth (TSB) and M9 medium with casamino acids (M9C) were examined. TSB grown cells enhance biofilm production compared with M9C-grown cells. Hyperspectral fluorescence images of the biofilm samples, in response to ultraviolet-A (320 to 400 nm) excitation, were acquired from approximately 416 to 700 nm. Visual evaluation of individual images at emission peak wavelengths in the blue revealed the most contrast between biofilms and stainless steel coupons. Two-band ratios compared with the single-band images increased the contrast between the biofilm forming area and stainless steel coupon surfaces. The 444/588 nm ratio images exhibited the greatest contrast between the biofilm formations and stainless coupon surfaces.

  11. Increased production of yersiniabactin and an anthranilate analog through media optimization.

    Science.gov (United States)

    Moscatello, Nicholas; Qi, Ruiquan; Ahmadi, Mahmoud Kamal; Pfeifer, Blaine A

    2017-05-25

    Yersiniabactin (Ybt) is a mixed nonribosomal peptide-polyketide natural product that binds a wide range of metals with the potential to impact processes requiring metal retrieval and removal. In this work, we substantially improved upon the heterologous production of Ybt and an associated anthranilate analog through systematic screening and optimization of culture medium components. Specifically, a Plackett-Burman design-of-experiments methodology was used to screen 22 components and to determine those contributing most to siderophore production. L-cysteine, L-serine, glucose, and casamino acids significantly contributed to the production of both compounds. Using this approach together with metabolic engineering of the base biosynthetic process, Ybt and the anthranilate analog titers were increased to 867 ± 121 mg/L and 16.6 ± 0.3 mg/L, respectively, an increase of ∼38 and ∼79-fold relative to production in M9 medium. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 2017. © 2017 American Institute of Chemical Engineers.

  12. Diversities in Virulence, Antifungal Activity, Pigmentation and DNA Fingerprint among Strains of Burkholderia glumae

    Science.gov (United States)

    Karki, Hari S.; Shrestha, Bishnu K.; Han, Jae Woo; Groth, Donald E.; Barphagha, Inderjit K.; Rush, Milton C.; Melanson, Rebecca A.; Kim, Beom Seok; Ham, Jong Hyun

    2012-01-01

    Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly correlated to virulence on onion bulb scales, suggesting that onion bulb can be a convenient alternative host system to efficiently determine the virulence of B. glumae strains. Production of toxoflavin, the phytotoxin that functions as a major virulence factor, was closely associated with the virulence phenotypes of B. glumae strains in rice. Some strains of B. glumae showed various levels of antifungal activity against Rhizoctonia solani, the causal agent of sheath blight, and pigmentation phenotypes on casamino acid-peptone-glucose (CPG) agar plates regardless of their virulence traits. Purple and yellow-green pigments were partially purified from a pigmenting strain of B. glumae, 411gr-6, and the purple pigment fraction showed a strong antifungal activity against Collectotrichum orbiculare. Genetic variations were detected among the B. glumae strains from DNA fingerprinting analyses by repetitive element sequence-based PCR (rep-PCR) for BOX-A1R-based repetitive extragenic palindromic (BOX) or enterobacterial repetitive intergenic consensus (ERIC) sequences of bacteria; and close genetic relatedness among virulent but pigment-deficient strains were revealed by clustering analyses of DNA fingerprints from BOX-and ERIC-PCR. PMID:23028972

  13. Thyroid peroxidase activity is inhibited by amino acids

    Directory of Open Access Journals (Sweden)

    D.P. Carvalho

    2000-03-01

    Full Text Available Normal in vitro thyroid peroxidase (TPO iodide oxidation activity was completely inhibited by a hydrolyzed TPO preparation (0.15 mg/ml or hydrolyzed bovine serum albumin (BSA, 0.2 mg/ml. A pancreatic hydrolysate of casein (trypticase peptone, 0.1 mg/ml and some amino acids (cysteine, tryptophan and methionine, 50 µM each also inhibited the TPO iodide oxidation reaction completely, whereas casamino acids (0.1 mg/ml, and tyrosine, phenylalanine and histidine (50 µM each inhibited the TPO reaction by 54% or less. A pancreatic digest of gelatin (0.1 mg/ml or any other amino acid (50 µM tested did not significantly decrease TPO activity. The amino acids that impair iodide oxidation also inhibit the TPO albumin iodination activity. The inhibitory amino acids contain side chains with either sulfur atoms (cysteine and methionine or aromatic rings (tyrosine, tryptophan, histidine and phenylalanine. Among the amino acids tested, only cysteine affected the TPO guaiacol oxidation reaction, producing a transient inhibition at 25 or 50 µM. The iodide oxidation inhibitory activity of cysteine, methionine and tryptophan was reversed by increasing iodide concentrations from 12 to 18 mM, while no such effect was observed when the cofactor (H2O2 concentration was increased. The inhibitory substances might interfere with the enzyme activity by competing with its normal substrates for their binding sites, binding to the free substrates or reducing their oxidized form.

  14. Mouse lung adhesion assay for Bordetella pertussis

    Energy Technology Data Exchange (ETDEWEB)

    Burns, K.A.; Freer, J.H. (Department of Microbiology, Alexander Stone Building, Bearsden, Glasgow, Scotland)

    1982-03-01

    The ability of Bordetella pertussis to adhere to cell surfaces has been demonstrated by adhesion to tissue culture cells and adhesion to chicken, hamster or rabbit trachea in organ culture. In this report a mouse lung assay for adhesion is described and the results obtained using two virulent strains of B. pertussis and their avirulent counterparts. These were a C modulation of one of the original virulent strains and a phase IV variant of the other virulent strain. Organisms were radiolabelled by adding 1 ..mu..Ci (37 K Bq) of (/sup 14/C)glutamic acid per 10 ml of culture medium before inoculation and incubation for 5 days. The lungs were washed by perfusion in situ with at least two volumes (1 ml) of sterile 1% (w/v) casamino acids. The percentage of the inoculated organisms retained in the lungs was determined, after removal of the lungs, by one of the following two methods: viable count or radioactive count. Results for both methods were expressed as the percentage of the inoculum retained in the lungs plus or minus one standard deviation.

  15. Effects of substrates and phosphate on INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride) and CTC (5-cyano-2,3-ditolyl tetrazolium chloride) reduction in Escherichia coli

    Science.gov (United States)

    Smith, J. J.; McFeters, G. A.

    1996-01-01

    The effects of substrates of primary aerobic dehydrogenases, and inorganic phosphate on aerobic INT and CTC reduction in Escherichia coli were examined. In general, INT produced less formazan than CTC, but INT (+) cell counts remained near values of CTC (+) cells. INT and CTC (+) cell numbers were higher than plate counts on R2A medium using succinate, formate, lactate, casamino acids, glucose, glycerol (INT only) and no substrate. Formate resulted in the greatest amount of INT and CTC formazan. Reduction of both INT and CTC was inhibited above 10 mmol l-1 phosphate, and this appeared to be related to decreased rates of O2 consumption. Formation of fluorescent CTC (+), but not INT (+) cells was also inhibited in a concentration dependent manner by phosphate above 10 mmol l-1. From light microscopic observations it appeared CTC formed increasing amounts of poorly or non-fluorescent formazan with increasing phosphate. Therefore, use of phosphate buffer in excess of 10 mmol l-1 may not be appropriate in CTC and INT reduction assays.

  16. Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production

    Energy Technology Data Exchange (ETDEWEB)

    Matsumoto, T.; Fukuda, H. [Kobe University, Kobe (Japan). Graduate School of Science and Technology, Division of Molecular Science; Takahashi, S.; Ueda, M.; Tanaka, A. [Kyoto University, Kyoto (Japan). Dept. of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering; Kaieda, M.; Kondo, A. [Kobe University, Kobe (Japan). Faculty of Engineering, Dept. of Chemical Science and Engineering

    2001-07-01

    Yeast whole-cell biocatalysts for lipase-catalyzed reactions were constructed by intracellularly overproducing Rhizopus oryzae lipase (ROL) in Saccharomyces cerevisiae MT8-1. The gene encoding lipase from R. oryzae IFO4697 was cloned, and intracellular overproduction systems of a recombinant ROL with a pro-sequence (rPRoROL) were constructed. When rProROL from R. oryzae IFO4697 was produced under the control of the 5'-upstream region of the isocitrate lyase gene of Candida tropicalis (UPR-ICL) at 30 C for 98 h by two-stage cultivation using SDC medium (SD medium with 2% casamino acids) containing 2.0% and 0.5% glucose, intracellular lipase activity reached levels up to 474.5 IU/l. These whole-cell biocatalysts were permeabilized by air-drying and used for the synthesis of methyl esters (MEs), a potential biodiesel fuel, from plant oil and methanol in a solvent-free and water-containing system. The ME content in the reaction mixture was 71 wt% after a 165-h reaction at 37 C with stepwise addition of methanol. These results indicate that an efficient whole-cell biocatalyst can be prepared by intracellular overproduction of lipase in yeast cells and their permeabilization. (orig.)

  17. Deferribacter thermophilus gen. nov., sp. nov., a novel thermophilic manganese- and iron-reducing bacterium isolated from a petroleum reservoir.

    Science.gov (United States)

    Greene, A C; Patel, B K; Sheehy, A J

    1997-04-01

    A thermophilic anaerobic bacterium, designated strain BMAT (T = type strain), was isolated from the production water of Beatrice oil field in the North Sea (United Kingdom). The cells were straight to bent rods (1 to 5 by 0.3 to 0.5 microns) which stained gram negative. Strain BMAT obtained energy from the reduction of manganese (IV), iron(III), and nitrate in the presence of yeast extract, peptone, Casamino Acids, tryptone, hydrogen, malate, acetate, citrate, pyruvate, lactate, succinate, and valerate. The isolate grew optimally at 60 degrees C (temperature range for growth, 50 to 65 degrees C) and in the presence of 2% (wt/vol) NaCl (NaCl range for growth, 0 to 5% [wt/vol]). The DNA base composition was 34 mol% G + C. Phylogenetic analyses of the 16S rRNA gene indicated that strain BMAT is a member of the domain Bacteria. The closest known bacterium is the moderate thermophile Flexistipes sinusarabici (similarity value, 88%). Strain BMAT possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus; therefore, we propose that this isolate should be described as a member of a novel species of a new genus, Deferribacter thermophilus gen. nov., sp. nov.

  18. A relaxed (rel) mutant of Streptomyces coelicolor A3(2) with a missing ribosomal protein lacks the ability to accumulate ppGpp, A-factor and prodigiosin.

    Science.gov (United States)

    Ochi, K

    1990-12-01

    A relaxed (rel) mutant was found among 70 thiopeptin-resistant isolates of Streptomyces coelicolor A3(2) which arose spontaneously. The ability of the rel mutant to accumulate ppGpp during Casamino acid deprivation was reduced 10-fold compared to the wild-type. Analysis of the ribosomal proteins by two-dimensional PAGE revealed that the mutant lacked a ribosomal protein, tentatively designated ST-L11. It was therefore classified as a relC mutant. The mutant was defective in producing A-factor and the pigmented antibiotic prodigiosin, in both liquid and agar cultures, but produced agarase normally. Production of actinorhodin, another pigmented antibiotic, was also abnormal; it appeared suddenly in agar cultures after 10 d incubation. Although aerial mycelium still formed, its appearance was markedly delayed. Whereas liquid cultures of the parent strain accumulated ppGpp, agar cultures accumulated only trace amounts. Instead, a substance characterized only as an unidentified HPLC peak accumulated intracellularly in the late growth phase, just before aerial mycelium formation and antibiotic production. This substance did not accumulate in mutant cells. It was found in S. lividans 66 and S. parvulus, but not in seven other Streptomyces species tested. The significance of these observations, and the relationship of the mutant to earlier rel isolates of Streptomyces is discussed.

  19. History of the Gas Turbine Engine in the United States: Bibliography

    Science.gov (United States)

    1988-05-11

    Fuel Consumption is the Major Consideration." Interavia 31 (May 1976):419. Grangier , M. &#34SNECMA M-53 Status Report." Interavia 31 (May 1976):409...34 Flying 107 (November 1980):37. Garrison, P.; and Collins, R. L. "Dead Engine: Single; Engine Failure: Twin." Eying 106 (November 1980):48. Grangier ...the 90’s." Asian Defense Journal 8 (August 1981):76. Grangier , M. &#34Single-Engine or Light Twin? A Difficult Choice for the Buyer." Interavia 36

  20. Late Bombardment of the Lunar Highlands Recorded in MIL 090034, MIL 090036 and MIL 090070 Lunar Meteorites

    Science.gov (United States)

    Park, J.; Nyquist, L. E.; Shih, C.-Y.; Herzog, G. F.; Yamaguchi, A.; Shirai, N.; Ebihara, M.; Lindsay, F. N.; Delaney, J.; Turrin, B.; Swisher, C., III

    2013-01-01

    The Kaguya mission detected small but widespread outcrops of nearly pure ferroan anorthosite in and around large impact basins on the Moon. Along with certain lunar rocks, highly feldspathic lunar meteorites such as MIL 090034 (M34), 090036 (M36), and 090070 (M70) may provide samples of this material. We have measured the Ar-40/Ar-39 release patterns and cosmogenic Ar-38 concentrations of several small (<200 microg) samples separated from M34,36, and 70. From petrographic observations concluded that "some of the clasts and grains experienced generations of modifications," a conclusion that we examine in light of our data.

  1. Napoleon I: A Bibliography.

    Science.gov (United States)

    1985-02-01

    64:255-270, February-November 1919. COOLEN, E.A. "Surprise," CAJ, 5:18-33, July 1951; 5:62-72, August 1951. DUTCHER, GEORGE M. &#34Tendencies and...SMIRNOFF, ALEXANDER "General Robert E. Lee and Napoleon," AQ, 14:121-9, 1927. STEPHENSON, GEORGE M. &#34Napoleon--Master Logistician," ALOG, 11:20-3...October 1964. GROBERT, J. "Une Relation de la Bataille des Pyramides," RdA, 83:421-30, 1913-1914. .4 WHITE, DIMITRI F. "Russo-Turkish Operations," MCG, 32

  2. The Nationwide Airport Noise Impact Model and Its Application to Regulatory Alternatives

    Science.gov (United States)

    1987-07-01

    1.032 2000 267,955 15,283 5.70 1.057 2010 283,238 Assumptions: Lifetime births per woman: 1.9 Life expectancy at birth , 2080: 81.0 Net immigration...Div., Civil Aeronautics Board, Feb. 1982. 11. Warren, D., "Area Equivalent Method on Lotus 1-2-3 T M &#34, Federal Aviation Administration, Report EE-84-12...both cases using the FAA Area Equivalent Model. ’Warren, D. "Area Equivalent Method on Lotus i-2-3 T M&#34. Federal Aviation Administration, Report EE-84

  3. International Symposium on New Trends in Photoelectrochemistry Held in Altavilla Milicia (Pa), Italy on 22-26 September 1991. Extended Abstracts

    Science.gov (United States)

    1991-09-01

    34 H. Tributsch, Hahn-Meitner-Institut, Berlin GERMANY 6.30 - 7.00 p.m.: &#34Some Basic Working Principles of Heterogeneous Photocatalysis by...Photokilling of Malignant Cells with Ultrafine TiO2 Powder" A. Fujishima, The University ofTokyo JAPAN 1O._00 - 10.30 a.m.: &#34Non-Linear Phenomena...etc. for stabilization, energy storage, photocatalysis and so on. Our works (Table) will be reviewed together with recent results. Ref) M.Kaneko et

  4. Managerial Risk Preferences for Below-Target Returns.

    Science.gov (United States)

    1980-08-01

    Selection (Boston: Martinus Nyhoff, in presp). 4. Fiahburn, P.C., "Mean-Risk Analysis with Risk Associated with Below Target Returns," American Economic Review (March...Holthausen, D.M., &#34Hedging and the Competitive Firm Under Price Uncer- tainty," American Economic Review (December 1979). 14. Kahneman, D. and A. Tversky

  5. Are Standing Joint Task Force Headquarters the First Step in Transforming Cold War Formations?

    Science.gov (United States)

    2002-05-01

    and General Staff College, 1995. Connaughton , Richard M. &#34Organizing British Joint Rapid Reaction Forces." Joint Force Quarterly. Autumn 2000, pp. 87...Margaret Roth, and Caleb Baker. Operation Just Cause: The Storming of Panama. New York: Lexington Books, 1991. Dunn, Brian J. "Rethinking Army-Marine

  6. Management Concerns for Optical Based Filing Systems

    Science.gov (United States)

    1990-03-01

    terminals, WORM Palo Alto, CA 94303 drives, optical jukeboxes, printers, scanners. Candi Technology Inc. Systems integrators providing 2354 Calle Del Mundo ...n.3, p. 68, 6 February 1989. Dortch, M., &#34A Storage Media Primer ", LAN Times, v.6, n.1, pp. 38-39, January 1989. Dukeman, John, "Optical Disk - A

  7. An Examination of Technology Transfer as a Tool for Management.

    Science.gov (United States)

    1986-03-01

    Berlo , R. K., Lamert, J. B., and Mertz, R. J., "Dimensions of Evaluating the Acceptability of Message Source", Public Opinion Quarterl, Vol. 33, 1979...1966. Carr-Harris, G. G. M., &#34The Information Scientist: Industry’s Link With Science and Technology", Industrial Canada, March 1964. Clark, David L

  8. Phase Difference/Time Delay Trace Functions and Their Application to Bearing Estimation in Arrays.

    Science.gov (United States)

    1979-12-01

    Am., V. 64, No. 6, p. 1602, December 1978. 123. Stremler , F. G., and Brown, W. M., &#34Phase Analysis in Multiple Sensor Receivers with High Signal To...Noise Ratio", IEEE Trans. Aerosp. Elect. Syst., V. AES-5, No. 2, p. 163, March 1969. 124. Stremler , F. G., "Estimation of Phase Differences in Multiple

  9. Products of the Hypochlorite Oxidation of Hydrazine Fuels

    Science.gov (United States)

    1989-06-01

    determined to be 0.030 M- s (Reference 52). Methyldiazene may react further in at least two distinct ways. Kosower and colleagues (Reference 51) observed...1972. 51. Kosowe -’, E.M., &#34Monosubstituted Diazenes (Diimides)," Accts. Chem. Research, Vol 4, pp. 193-198, 1971. 52. Tsuji, T., and Kosower , E.M

  10. Census Report: Volume VI, 1987 through 1992. Sanitized Version.

    Science.gov (United States)

    2007-11-02

    0599 GUERNSEY, JEFFREY S 0754 GUERRA , PAUL 0599 GUIDA, RICHARD A 0599 GUITERREZ, EDUARDO 0599 GUNDERSON, ALLAN W 0599 (TTTJ’M&#34N ,Tr’Frr y L - 0638...PATTERSON, JOHN E 0837 PATTON, TERRY R 0903 PAYNE, DEBORAH N 0972 PAYNE, WILLIAM R 0978* PAZ , EDWARD L 0978* PEACE, BOBBY J 0599 PEAKE, DARRELL D 0975

  11. Application of Rate Theory to Accelerated Durability Testing of Structural Adhesives

    Science.gov (United States)

    1980-03-01

    interest of processing economy can be identified. This information can then be fed back into the system design process and the analyses repeated to...London, F., Problemme der Modernon Physik, Sommerfeld Festchrift. 1931 Eyring, H. and Polanyi, M., &#34 Uber Einfache Gasreaktionen", Z. Physic, Chem

  12. Tactical Level Supervision: Ensuring Success by Foiling Failure

    Science.gov (United States)

    1991-12-20

    Ibid, p. 48. 35 Webster’s Ninth New Collegiate Dictionary, ( Meriam -Webster Inc., Springfield, Ma., 1986), p. 1185. 44 Austin, Nancy and Peters, Tom. A...Company, Inc., 1981. Webster’s Ninth New Collegiate Dictionary. Springfield, Ma: Meriam -Webster Inc., 1986. Articles Dubik, James M. &#34Supervision

  13. On the Near Surface Population of Oceanic Microbubbles

    Science.gov (United States)

    1989-06-01

    and J = 5. The slope of Monahan’s curves indic acs an r-4-3 dependance , approximately. During a number of the experimental runs of Walsh and...O’Muircheartaigh, I.G. Coverage Dependance on Wind-Speed". J. Phys. Oceanogr., 10, pp.2094-2099, 1980 8 Tate, P.M. &#34The Distribution of Ocean Microbubbles

  14. Neural Network Based Propulsion System Fault Diagnostics for the NPS AUV II

    Science.gov (United States)

    1992-06-01

    improved. Work in the area of system fault detection has been undertaken by several investigators in the past including Himmelblau , (1978) and Pau...Underwater Vehicle Testbed: Design and Experimental Verification", Naval Engineers Journal, pp. 191-202, May 1992. 59 Himmelblau , D.M., &#34Fault Detection

  15. Parallel Computation for Developing Nonlinear Control Procedures.

    Science.gov (United States)

    1981-07-01

    and Himmelblau E35] indicate that the BFS rule is generally preferable to the DFP and SR1 updates because of its reliability of convergence for a wide...New York, 1972, pp. 149-170. 35. Himmelblau , D. M., &#34A Uniform Evaluation of Unconstrained Optimiza- tion Techniques," in: Nurierical Methods for

  16. Convergence Behaviour of Some Iteration Procedures for Exterior Point Method of Centres Algorithms,

    Science.gov (United States)

    1979-02-01

    in Reference 4, while Staha and Himmelblau 9 reported very favourably on their application of Newton’s method to the exterior point method of centres...34. .4cfta Poll- technica Scandinavica, Trondheim, 13 (1966). 9. Staha, R. L., and Himmelblau . D. M., &#34Evaludtion of Constrained Nonlinear Programming

  17. Identification of Large Space Structures on Orbit

    Science.gov (United States)

    1986-09-01

    problem is thus the inverse of the analysis problem for dynamical systems with which the structural engineers are quite familiar . However, whereas the...Belgrade, Yugoslavia. Gagliardt, R. M., &#34Input Selection for Parameter Identification in Discrete Systems", IEEE Trama . Auto. Cont., Vol. AC-12, Pp

  18. Survey of Biodegradation of Electronic Components and Associated Testing Using Decontamination Solution

    Science.gov (United States)

    1991-08-01

    Medical Research Laboratory, Wright-Patterson Airforce Base, OH, November 1974, UNCLASSIFIED Report. Rubidge, T., Growth of Cladosporium resinae ...Report. A-5 METALS AvLlon, E.S., and Rosales, B.M., &#34Corrosion of AA 7075 Aluminum Alloy in Media Contaminated with Cladosporiim resinae ," Corrosion

  19. Managing the Ethnic Factor in the Russian and Soviet Armed Forces. An Historical Overview,

    Science.gov (United States)

    1982-07-01

    Stephen M., &#34The Democratic Counterrevolution: Komuch and the Civil War on the Volga," Canadian-American Slavic Studies , Vol. VII, No. 4, Winter 1973...34 Canadian Slavic Studies , Vol. 4, No. 4, Winter 1970, pp. 721- 735. Mackintosh, Malcolm, Juggernaut, Macmillan Company, New York, 1967. Madatov

  20. Visionary Leadership

    Science.gov (United States)

    1993-06-04

    was not one to seek inspiration in a wide array of sources; he read newspapers, the Bible , and the Bard (especially "Macbeth," "Hamlet" and "Richard...DC: Government Printing Office 1989. Wall, James M. &#34Wanted: Leaders With Vision." Christian Century, 23 September 1987, 779. Waller, Calvin General

  1. Suspended Sediments Measured in the Surf Zone.

    Science.gov (United States)

    1977-09-01

    described by Komar and Inman (1970). Sus- pended sediments were measured in situ by swimmers using a mechanical water sampling device which...PEÜüENC’-1 fH* 39 imt ^^m &#34/• ffr- 7 If) \\ h A /’ kl v \\ n/\\ i > ^ U, Vectra of onshore-offshore flow vs. nephelcmeter

  2. MASTER: high amplitude (>6.8m) optical transient and new OT

    Science.gov (United States)

    Gress, O.; Pogrosheva, T.; Lipunov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vladimirov, V.; Shumkov, V.; Kuvshinov, D.; Ivanov, K.; Budnev, N.; Tlatov, A.; Senik, V.

    2017-02-01

    MASTER-Tunka auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 19h 12m 48.12s +37d 49m 34.6s on 2017-01-31.86338 UT. The OT unfiltered magnitude is (limit 18.4m).

  3. Slow and Steady Wins the Race: A Randomized Clinical Trial of Acceptance and Commitment Therapy Targeting Shame in Substance Use Disorders

    Science.gov (United States)

    Luoma, Jason B.; Kohlenberg, Barbara S.; Hayes, Steven C.; Fletcher, Lindsay

    2012-01-01

    Objective: Shame has long been seen as relevant to substance use disorders, but interventions have not been tested in randomized trials. This study examined a group-based intervention for shame based on the principles of acceptance and commitment therapy (ACT) in patients (N = 133; 61% female; M = 34 years old; 86% Caucasian) in a 28-day…

  4. Spectroscopy of Non-Agglomerated Particles in the Condensed Phase

    Science.gov (United States)

    1988-02-01

    optical properties of solids are...Academic Press. New York (1985). pp. 214. 230-231. 5. Stern. F., "Elementary Theory of the Optical Properties of Solids ." Solid State Physics 15. 299-308...1964). 6. Cardona, M.. &#34Optical Constants of Insulators: Dispersion Relations," in Optical Properties of Solids , S. Nudelman and S. S. Mitra

  5. Dicty_cDB: Contig-U06814-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 04074320. 34 0.54 2 ( AC109637 ) Homo sapiens BAC clone RP11-325A18 from 2, comple... 34 0.60 2 ( DJ051139 ) HIGH EFFICIENCY... GENE TRANSFER AND EXPRESSION IN M... 34 0.65 2 ( DI107068 ) HIGH EFFICIENCY

  6. Northeast Artificial Intelligence Consortium Annual Report 1987. Volume 6. Artificial Intelligence Applications to Speech Recognition

    Science.gov (United States)

    1989-03-01

    Waibel (1981), in addition to connected word recognition by Myers et al. (1981), Ney (1984), and Watari (1986). An unknown utterance and a reference...Techniques in Isolated Word Speech Recognition Systems", Research paper, Carnegie-Mellon University, CMU-CS-81-125, June 1981. Watari , M. &#34New DP

  7. A Survey of Productivity in the Construction Industry: Measurement and Causes.

    Science.gov (United States)

    1987-12-01

    increase in number rather than decrease [ Arditi , 1985 and Choromokos, 1981). RESEARCH AND DEVELOPMENT Numerous studies have examined the relationship between...Center, Houston, Texas 77024, 1987 ( Arditi ,1985) Arditi , David M., &#34Construction Productivity Improvement", Journal of Construction Enqineerinq and

  8. Risk and Protective Factors for Physical and Emotional Intimate Partner Violence against Women in a Community of Lima, Peru

    Science.gov (United States)

    Mayorga, Magaly Noblega

    2012-01-01

    This article shows risk and protective factors for both physical and emotional intimate partner violence (IPV) against women. The study was carried out in a shanty town of Lima, Peru, which has a strong community organization. One hundred ninety-two women between 25 and 59 years old (M = 34.09, SD = 6.5) were interviewed; 44.3% had secondary…

  9. An Asymptotic Result for the Multi-Stage Weapon-Target Allocation Problem

    Science.gov (United States)

    1990-02-01

    Matlin [8] and Eckler and Burr [4] give reviews of the material on weapons allocation problems. Howevex, in these studies, very little emphasis is given...1986. [8] Matlin , S. M., &#34A Review of the Literature on the Missile-Allocation Problem," Operation• . -Research, vol. 18, pp. 334-373, 1970. 18

  10. The Shock and Vibration Digest, Volume 17, Number 11

    Science.gov (United States)

    1985-11-01

    3), pp Subjected to Impact," Computers Struc., 1- 101-104 (Fall 1P82). (5/6), pp 807-813 (1984). 3. Lal, K.M., &#34Low Velocity Transverse 13. Caprino ...Chen, J.K. and Sun, C.T., ’Impact 25. Caprino , G., "Residual Strength Predic- Response of Buckled Composite Laminates," tion of Impacted CFRP Laminates

  11. Multi-Disciplinary Research for High Energy Chemical Lasers, Closed-Cycle ElectricOIL Technology

    Science.gov (United States)

    2007-10-31

    85 W. In (b) the scale is expanded to illustrate steady-state 02(b) concentrations. -1 I.1 koums ’) Sets of data from double discharge...Lasers and Intense Beam Applications 11, San Jose, CA, Jan , 1999. Morgan, W.L. and Penetrante, B.M., &#34ELENDIF: A Time-Dependent Boltzmann Solver

  12. Maintenance Categories - A Substudy of the Maintenance Support Structure for Contingency Forces Study (ACN 21012).

    Science.gov (United States)

    1977-06-23

    Major Albert B. Hutton, Major James W. Jeter, Jr., Mr. L. Conrad Davis, CPr John C. Wilkins, CPT Willian C., Bayer , Mr. Prancis E. Vogt, CW2 Rocbert M...34Maintenance Support Positive," At!y L •istician, Vol. 2, No. 2, (March - April 1970). 3. Bell, Raymond , et rd. Vehicle Aver e Useful Life Study for Truck

  13. Intelligence/Electronic Warfare (IEW) Direction-Finding and Fix Estimation Analysis Report. Volume 2. Trailblazer

    Science.gov (United States)

    1985-12-20

    Smart, W. M., &#34Text-Book on Spherical Astronomy," Cambridge University Press, 1962. 5. Hutchinson, C. L., "The ARRL Handbook for the Radio Amateur...Algorithms," Jet Propulsion Laboratory, Pasadena, CA, 4.- 09 July 1985. S2. Korn and Korn, "Mathematical Handbook for Scientists and Engineers," Second Edition

  14. Proceedings of the International Symposium on the Structure and Properties of Dislocations in Semiconductors (6th) held in Oxford (England) 5-8 April 1989: Structure and Properties of Dislocations in Semiconductors 1989

    Science.gov (United States)

    1989-04-08

    1983 J. Appl. Phys. 54, 161 Maeda K. 1985 in "Dislocations in solids" Yamada Science foundation University of Tokio press, 425 Masut R., Penchina C.M...34 material. 2. EXPERIMENTAL Two types of 6H SiC single crystals (doping levels unknown, one type blue -black and the other yellow in colour) with (0001

  15. Nitramine Monopropellant Deflagration and General Nonsteady Reacting Rocket Chamber Flows.

    Science.gov (United States)

    1980-01-01

    University, Department of Aerospace, Princeton, NJ, 1973. 68. Maltsev , V. M. Law, R. J., Ryan, N. W., Bare, A. D., and Summerfield, M., &#34on the...closeness of the HMX and RDX points; no attempt was made to correlate the HMX results. -189- DB, LUMINOUS FLAMtE FRONT, MEASURED BY MALTSEV MEASURED

  16. Convective Ignition of Propellant Cylinders in a Developing Cross-Flow Field.

    Science.gov (United States)

    1980-09-01

    which have strong lines at around 3750-3800 A, and 3.950 A, respectively. Indeed, these spectra were always found in Maltsev 1103J experiments with... Maltsev , V. M. and Summerfield, M., &#34Investigation of the Spectral Radiation Characteristics of the Flame-Jet of Nitrate-Ester Propellants Containing

  17. Weapons Systems Analysis

    Science.gov (United States)

    1981-10-01

    Defense Review, 10, 43-47 (1977). [429] Brains, S. J., Game Theory and Politics, The Free Press, New York, New York (1975). [430] Bresson , M. M., &#34Les...Study in Conflict and Cooperation, University of Michigan Press, Ann Arbor, Michigan (1965). [505] Roberts , D. M. and J. C. Gittins, "The Search For An

  18. Operation TEAPOT, 1955 Continental Nuclear Weapons Test Series

    Science.gov (United States)

    1981-11-23

    Engineering Co., Inc.) ATTN: Mr. Richard V. Nutley 2753 S. Highland P.O. Box 14100 Phone: (702) 734-3194 Las Vegas, Nevada 89114 FTS: 598-3194 Source...DASA. WT-1225. 10/28/59. 98 Pages. (A05) AD 460 282.* 236. McConnel, E.; Sampson, G. 0.; Sharf , J. M. &#34The Effect of Nuclear Explosions on Commercially

  19. Optimism and Cardiovascular Function in Children with Congenital Heart Disease

    Science.gov (United States)

    2010-02-17

    34"’_, ..... ’ 1UIIV: ~...... 01 _of _1m&#34 ....... otion 01 _, 0tId lor 1110 "’ ...... ’OIY ,tf! OrtI "g 01 me<k .. ,_tIoo. &1 .. QUIto<I.., ..... 101.."".. .... "’"" bo

  20. Computational Aerodynamics Based on the Euler Equations (L’aerodynamique Numerique a Partir des Equations d’Euler)

    Science.gov (United States)

    1994-01-01

    dquations d’Euler) Edited by * S J.W. SLOOFF National Aerospace Laboratory NLR Anthony Fokkerweg 2 1059 CM Amsterdam Netherlands Dr. W. SCHMIDT Air Vehicle...CHAKRAVARTHY. S. R., RIBA , W.* * T.. BYERLY. J. and DRESSER. H. S.. "Multi-Zone Euler 41. DESLANDES R.M.. &#34Theoretisebe bestimmung der Marching Technique

  1. Failure Considerations in Composite Systems Based on 3D Micromechanical Stress Fields: Part A

    Science.gov (United States)

    1992-12-01

    Introduction to Composite Materials ", Cam- bridge University Press. Li, P.C...Arbitrary Thickness", University of Utah Technical Report. Hull, D., 1981, "An Introduction to Composite Materials ", Cambridge University Press. Keer, L.M...34 An Introduction to Composite Materials ,Cambridge University Press. 10. Folias, E. S., (1974 ),Thin-Shell Structures , Edited by Fung and

  2. Slow and Steady Wins the Race: A Randomized Clinical Trial of Acceptance and Commitment Therapy Targeting Shame in Substance Use Disorders

    Science.gov (United States)

    Luoma, Jason B.; Kohlenberg, Barbara S.; Hayes, Steven C.; Fletcher, Lindsay

    2012-01-01

    Objective: Shame has long been seen as relevant to substance use disorders, but interventions have not been tested in randomized trials. This study examined a group-based intervention for shame based on the principles of acceptance and commitment therapy (ACT) in patients (N = 133; 61% female; M = 34 years old; 86% Caucasian) in a 28-day…

  3. The Search for Peace and Security: The Case of Botswana

    Science.gov (United States)

    1994-06-01

    o" wwapw I hoar Par ,epý. schwagm t6C tie far .evusut amm seara nug ejusaft do&n saame. gaUheng sad .aammag th. dar moo edad cad okcig and .viawas the...Richard D. Irwin, 1984. Defense & Foreign Affairs Handbook. 1990-1991, International Media Corporation, 1990. du Plessis, A and Hough, M., &#34Civil War in

  4. The RISC (Reduced Instruction Set Computer) Architecture and Computer Performance Evaluation.

    Science.gov (United States)

    1986-03-01

    8 )1; II;-21(82 ? 1JV , *~A 1*-e eo I Q .f ’ . - . - . .> - Approved for public release; distribution is unlimited. The RISC Architecture and Computer...1000 Lisboa Portugal 6. Manuel Pedrosa de Barros 4 Celula 5 Bloco 5 Lote D, 3 Direito 2795 Linda-a-Velha Portugal t~m &#34 96" ..... ...... |f

  5. Mathematical Modelling of Laser/Material Interactions.

    Science.gov (United States)

    1983-11-25

    translated to the model input. Even an experimental mode print can also be digitalised for the model. In trying to describe high order modes matliematically...4. Mazumder J. Steen W.M. &#34Welding of Ti 6al - 4V by continuous wave CO2 laser". Metal construction Sept. 1980 pp423 - 427. 5. Kogelnik H, Li.T Proc

  6. Toward the Development of a Navy-Census Occupational Dictionary

    Science.gov (United States)

    1975-03-28

    Research Prof. Paul F. Lazarsfeld # m ARMY OAS (M&RA), Manpower AFHRL Pers Res Div, Lackland AFB Wright-Patterson AFB Dr. G. A. Eckstrand...and Sciences, The George Washington University. [3] HODGE, ROBERT W. and SIEGEL, PAUL M. &#34The Classification of Occu- pations: Some Problems of

  7. The Role of Osteoblast-Derived Cytokines in Bone Metastatic Breast Cancer

    Science.gov (United States)

    2009-01-01

    human metastatic breast cancer cells." Cancer Res. 15: 2003. 2631- 2637. 59. Tetsuya, M. &#34Toll-like receptor signals and periodontitis ." Annals of...1994). Estrogens, cytokines, and the pathophysiology of osteoporosis. In P. O. Kohler (Ed.) Current opinion in endocrinology and diabetes (pp. 275–281

  8. Strong earthquake activity all over the world and strong-moderate earthquake activity within and near China (February, 2003 ~ March, 2003)

    Institute of Scientific and Technical Information of China (English)

    陈培善

    2003-01-01

    @@ Illustration All the data in this catalog are chosen from the "Preliminary Seismological Report of Chinese Seismic Stations" (Its abbreviation is "Monthly Report"). The catalog includes the events of M34.7 in and near China and M36 all over the world. The "Monthly Report" is monthly compiled by the Ninth Section of Institute of Geophysics, CSB.

  9. Calibration and Extension of a Discrete Event Operations Simulation Modeling Multiple Un-Manned Aerial Vehicles Controlled by a Single Operator

    Science.gov (United States)

    2013-03-01

    CALIBRATION AND EXTENSION OF A DISCRETE EVENT OPERATIONS SIMULATION MODELING MULTIPLE UN-MANNED AERIAL VEHICLES...DISCRETE EVENT OPERATIONS SIMULATION MODELING MULTIPLE UN-MANNED AERIAL VEHICLES CONTROLLED BY A SINGLE OPERATOR THESIS Presented to the...ENV-13-M-34 CALIBRATION AND EXTENSION OF A DISCRETE EVENT OPERATIONS SIMULATION MODELING MULTIPLE UN-MANNED AERIAL VEHICLES CONTROLLED BY

  10. Impact Dynamics: Theory and Experiment

    Science.gov (United States)

    1980-10-01

    Friedricks, K.O., and Lewy, H., " Uber die Partiallen Differenzen-gleichungen der Matematischen Physik," Math. Ann. 100 1928. 38. Van Thiel, M., &#34Compendium of...92647 65 DISTRIBUTION LIST No. of No. of Copies Organization Copies Organization 1 New Mexico Institute of 1 US Steel Corporation Mining and

  11. Conflict Handling Styles and Project Manager Effectiveness

    Science.gov (United States)

    1988-09-01

    hostile conflict among its functional departments and also to stimulate differences and capitalize on them by productive ,-, .-,• , ,,,,m w w m m ~ml m m...Conflict: A Model for Diagnosis and Intervention," Psycological Reports. 44: 1323-1344 (June 1979). 31. Steers, Richard M. &#34Problems in the Measurement of

  12. The Effects of Grain Size on the Martensitic Transformation in Copper-Zinc-Aluminum Shape Memory Alloys.

    Science.gov (United States)

    1982-12-01

    production or nearly so. In the medical arts these alloys are being exploited for their SME properties as orthodontic appliances, intercranial aneurism...treatment is applicable to the Cu-Zn-Al alloys. 60 LIST OF REFERENCES 1. Wayman, C. M., &#34Some Applications of Shape Memory Alloys," Journal of Metals, pp

  13. Experiments, Theory, and Simulation on the Evolution of Fabric in Granular Materials

    Science.gov (United States)

    1992-07-27

    sac ± s+"ai)dw (51) where the integration is over the solid angle subtended by the region of sliding. We note that ef = 0, so that there is no...Deformation," Intl. J. Plast., 7, 141-160, 1991. [4] Arminjon, M. &#34Sur le Champ de Rotation des Cristaux dans un Poly- cristal Dform6 Plastiquement

  14. Alternative Control Technologies: Human Factors Issues

    Science.gov (United States)

    1998-10-01

    vertical vibration to the 29,4, 1980, pp 462-466. head and shoulders of seated men", Royal Aircraft Lee, J. M.; Chartier , V. L.; Hartmann, D. P.; Lee, G...Suarez, P. F., Rogers , S., K., Ruck, D. W., for Effective Human-Computer Interaction", 2nd edition, Arndt, C., and Kabrisky, M., &#34A facial feature

  15. Optimized Hyperthermia Treatment of Prostate Cancer Using a Novel Intracavitary Ultrasound Array

    Science.gov (United States)

    2006-01-01

    Seegenschmiedt, M. and Saur, R., Interstitial and intracavitary thermoradiotherapy Berlin: Springer-Verlag, 1993. [3] Seegenschmiedt, M., Fessenden , P., and...and eegenschmiedt, M., &#34Interstitial heating technologies," in Seegenschmiedt MH, Fessenden P, and ernon CC (eds.) Principles and practices of...thermoradiotherapy Berlin: Springer-Verlag, 1993. 5. Seegenschmiedt, M., Fessenden , P., and Vernon, C., Principles and practices of thermoradiotherapy and

  16. Optimization of the Agrobacterium-Mediated Transformation System on Upland Rice%影响农杆菌介导旱稻转化效率主要因素的研究

    Institute of Scientific and Technical Information of China (English)

    石云鹭; 丁在松; 张彬; 张桂芳; 赵明

    2007-01-01

    针对旱稻在基因工程中转化效率低这一难题,以4个旱稻品种为对象,对农杆菌介导的遗传转化的各个步骤进行了优化试验,结果表明NB(N6 macro elements;B5 micro elements and vitamins;proline 500 mg L-1,L-Glutamine 500 mg L-1,casamino acid 300 mg L-1,sucrose 30 g L-1,2,4- D 2 mg L-1,agar 8 g L-1,pH 5.8)培养基适合于旱稻愈伤组织诱导;AAM-AS (Na2HPO4·2H2O 339.2 mg L-1; KCl 2.95 g L-1; MgSO4·7H2O 500 mg L-1; CaCl2 114.4 mg L-1; MnSO4·4H2O 10 mg L-1; H3BO3 3 mg L-1; ZnSO4·7H2O 2 mg L-1; KI 0.75 mg L-1; NaMO4 0.25 mg L-1;CuSO4·5H2O 0.0387 mg L-1;CoCl2·6H2O 0.025 mg L-1; VB1 0.5 mg L-1;VB6 0.5 mg L-1;烟酸0.5 mg L-1;肌醇100 mg L-1; 甘氨酸7.5 mg L-1;精氨酸174 mg L-1;谷氨酰铵876 mg L-1; casamino acid 500 mg L-1; 蔗糖68.5 g L-1; 葡萄糖35 g L-1; AS 200 μmol L-1 pH 5.2)是农杆菌的最佳重悬液;对NPT(new plant type, NPT)这种侵染后易水渍化品种, 滤纸法共培养可将其抗性愈伤发生率提高15倍以上;筛选及分化前对抗性愈伤组织进行2 d的干燥培养可显著加快愈伤组织分化进程;在分化培养基中加入AgNO3有助于提高分化率;而短期高浓度的CuSO4处理可显著提高分化率.利用此体系获得了一大批PCR鉴定阳性的转基因植株; Southern blot检测证明外源基因大多数以单拷贝形式整合在转基因植株中.

  17. Thermocrinis jamiesonii sp. nov., a thiosulfate-oxidizing, autotropic thermophile isolated from a geothermal spring

    Energy Technology Data Exchange (ETDEWEB)

    Ong, John C.; Dodsworth, Jeremy A.; Hedlund, Brian P.; Dohnalkova, Alice C.; Williams, Amanda J.

    2015-12-01

    An obligately thermophilic, chemolithotrophic, microaerophilic bacterium, designated strain GBS1T, was isolated from the water column of Great Boiling Spring, Nevada, USA. Thiosulfate was required for growth. Although capable of autotrophy, growth of GBS1T was enhanced in the presence of acetate, peptone, or Casamino acids. Growth occurred at 70-85 °C with an optimum at 80 °C, at pH 6.5-7.75 with an optimum at pH 7.25, at 0.5-8% oxygen with an optimum at 1-2%, and at ≤200 mM sodium chloride. The doubling time under optimal growth conditions was 1.3 hrs, with a final cell density of 6.2±0.5 x 107 cells/mL. Non-motile, rod-shaped cells 1.4-2.4 x 0.4-0.6 µm occurred singly or in pairs. Major cellular fatty acids (>5% of total) were C20:1ω9c (44.8%), C18:0 (26.0%), C16:0 (9.9%) and C20:0 (5.4%). Phylogenetic analysis of the GBS1T 16S rRNA gene sequence indicated an affiliation with Thermocrinis ruber and other Thermocrinis spp., but comparisons of 16S rRNA gene identity (≤97.10%) and in silico estimated DNA-DNA hybridization values (≤18.4%) with Thermocrinis spp. indicate that his strain is distinct from described species. Based on phenotypic, genotypic, and phylogenetic characteristics, the name Thermocrinis jamiesonii sp. nov. is proposed, with GBS1T (= JCM 19133T = DSM 27162T) as the type strain.

  18. Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum

    Energy Technology Data Exchange (ETDEWEB)

    Svetlitshnyi, V.; Wiegel, J. [Univ. of Georgia, Athens, GA (United States); Rainey, F. [German Collection of Microorganisms and Cell Cultures, Braunschweig (Germany)

    1996-10-01

    Three strains of an anaerobic thermophilic organoheterotrophic lipolytic alkalitolerant bacterium, Thermosyntropha lipolytica gen. nov., sp. nov. (type strain JW/VS-264{sup T}; DSM 11003) were isolated from alkaline hot springs of Lake Bogoria (Kenya). The cells were nonmotile, non-spore forming, straight or slightly curved rods. At 60{degrees}C, the pH range for growth determined at 25{degrees}C [pH{sup 25{degrees}C}] was 7.15 to 9.5, with an optimum between 8.1 and 8.9 (pH{sup 60{degrees}C} of 7.6 and 8.1). At a pH{sup 25{degrees}C} of 8.5 temperature range for growth was from 52 to 70{degrees}C, with an optimum between 60 and 66{degrees}C. The shortest doubling time was around 1 h. In pure culture the bacterium grew in a mineral base medium supplemented with yeast extract, tryptone, Casamino Acids, betaine, and crotonate as carbon sources, producing acetate as a major product and constitutively a lipase. During growth in the presence of olive oil, free long-chain fatty acids were accumulated in the medium but the pure culture syntrophic coculture (Methanobacterium strain JW/VS-M29) the lipolytic bacteria grew on triacylglycerols and linear saturated and unsaturated fatty acids with 4 to 18 carbon atoms, but glycerol was not utilized. Fatty acids with even numbers of carbon atoms were degraded to acetate and methane, while from odd-numbered fatty acids 1 mol of propionate per mol of fatty acid was additionally formed. 16S rDNA sequence analysis identified Syntrophospora and Syntrophomonas spp. as closest phylogenetic neighbors.

  19. Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum.

    Science.gov (United States)

    Svetlitshnyi, V; Rainey, F; Wiegel, J

    1996-10-01

    Three strains of an anaerobic thermophilic organoheterotrophic lipolytic alkalitolerant bacterium, Thermosyntropha lipolytica gen. nov., sp. nov. (type strain JW/VS-265T; DSM 11003), were isolated from alkaline hot springs of Lake Bogoria (Kenya). The cells were nonmotile, non-spore forming, straight or slightly curved rods. At 60 degrees C the pH range for growth determined at 25 degrees C [pH25 degrees C] was 7.15 to 9.5, with an optimum between 8.1 and 8.9 (pH60 degrees C of 7.6 and 8.1). At a pH25 degrees C of 8.5 the temperature range for growth was from 52 to 70 degrees C, with an optimum between 60 and 66 degrees C. The shortest doubling time was around 1 h. In pure culture the bacterium grew in a mineral base medium supplemented with yeast extract, tryptone, Casamino Acids, betaine, and crotonate as carbon sources, producing acetate as a major product and constitutively a lipase. During growth in the presence of olive oil, free long-chain fatty acids were accumulated in the medium but the pure culture could not utilize olive oil, triacylglycerols, short- and long-chain fatty acids, and glycerol for growth. In syntrophic coculture (Methanobacterium strain JW/VS-M29) the lipolytic bacteria grew on triacylglycerols and linear saturated and unsaturated fatty acids with 4 to 18 carbon atoms, but glycerol was not utilized. Fatty acids with even numbers of carbon atoms were degraded to acetate and methane, while from odd-numbered fatty acids 1 mol of propionate per mol of fatty acid was additionally formed. 16S rDNA sequence analysis identified Syntrophospora and Syntrophomonas spp. as closest phylogenetic neighbors.

  20. Effects of chemical speciation in growth media on the toxicity of mercury(II).

    Science.gov (United States)

    Farrell, R E; Germida, J J; Huang, P M

    1993-05-01

    The toxicity of metals, including mercury, is expressed differently in different media, and the addition of soluble organics to the growth medium can have a significant impact on bioassay results. Although the effect of medium composition on metal toxicity is generally attributed to its effect on metal speciation (i.e., the chemical form in which the metal occurs), the importance of individual metal-ligand species remains largely unclear. Here, we report the results of a study that investigated, both experimentally and from a modeling perspective, the effects of complex soluble organic supplements on the acute toxicity (i.e., 50% inhibitory concentration [IC50]) of mercury to a Pseudomonas fluorescens isolate in chemically well-defined synthetic growth media (M-IIX). The media consisted of a basal inorganic salts medium supplemented with glycerol (0.1%, vol/vol) and a variety of common protein hydrolysates (0.1%, vol/vol), i.e., Difco beef extract (X = B), Casamino Acids (X = C), peptone (X = P), soytone (X = S), tryptone (X = T), and yeast extract (X = Y). These were analyzed to obtain cation, anion, and amino acid profiles and the results were used to compute the aqueous speciation of Hg(II) in the media. Respirometric bioassays were performed and IC50s were calculated. Medium components varied significantly in their effects on the acute toxicity of Hg(II) to the P. fluorescens isolate. IC50s ranged from 1.48 to 14.54 micrograms of Hg ml-1, and the acute toxicity of Hg(II) in the different media decreased in the order M-IIC > M-IIP > M-IIB > M-IIT > M-IIS > M-IIY.(ABSTRACT TRUNCATED AT 250 WORDS)

  1. Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction

    Science.gov (United States)

    Moser, D. P.; Nealson, K. H.

    1996-01-01

    The growth of bacteria by dissimilatory elemental sulfur reduction is generally associated with obligate anaerobes and thermophiles in particular. Here we describe the sulfur-dependent growth of the facultatively anaerobic mesophile Shewanella putrefaciens. Six of nine representative S. putrefaciens isolates from a variety of environments proved able to grow by sulfur reduction, and strain MR-1 was chosen for further study. Growth was monitored in a minimal medium (usually with 0.05% Casamino Acids added as a growth stimulant) containing 30 mM lactate and limiting concentrations of elemental sulfur. When mechanisms were provided for the removal of the metabolic end product, H2S, measurable growth was obtained at sulfur concentrations of from 2 to 30 mM. Initial doubling times were ca. 1.5 h and substrate independent over the range of sulfur concentrations tested. In the cultures with the highest sulfur concentrations, cell numbers increased by greater than 400-fold after 48 h, reaching a maximum density of 6.8 x 10(8) cells ml-1. Yields were determined as total cell carbon and ranged from 1.7 to 5.9 g of C mol of S(0) consumed-1 in the presence of the amino acid supplement and from 0.9 to 3.4 g of C mol of S(0-1) in its absence. Several lines of evidence indicate that cell-to-sulfur contact is not required for growth. Approaches for the culture of sulfur-metabolizing bacteria and potential ecological implications of sulfur reduction in Shewanella-like heterotrophs are discussed.

  2. The 24-kDa protein from Fusarium oxysporum f.sp. erythroxyli: occurrence in related fungi and the effect of growth medium on its production.

    Science.gov (United States)

    Bailey, B A; Jennings, J C; Anderson, J D

    1997-01-01

    A 24-kDa protein that elicits ethylene production and necrosis in leaves of dicotyledonous plants was previously purified from culture filtrates of Fusarium oxysporum Schlechtend:Fr. f.sp. erythroxyli. Antisera to the denatured 24-kDa protein detected 2.5 ng of the 24-kDa protein on Western blots at 100000-fold dilutions. The antisera cross-reacted with a 24-kDa protein on Western blots of culture filtrates from three other F. oxysporum formae speciales. Of seven Fusarium species, only F. oxysporum, F. acuminatum Ellis and Kellerm., and F. avenaceum (Fr.:Fr.) Sacc. isolates produced an antigenically related 24-kDa protein. Although there were differences in the profiles of proteins extracted from stems of coca (Erythroxylum coca var. coca L. Lam.) infected with F. oxysporum f.sp. erythroxyli compared with uninfected stems, antisera to the 24-kDa protein did not cross-react with any proteins from the infected coca stems. For the fungal isolates studied, the best medium tested for production of the 24-kDa protein contained 1% sucrose and 1% asparagine. Biological activity of the F. oxysporum culture filtrates on sweet basil leaves was consistently correlated with the presence of the 24-kDa protein. Production of the 24-kDa protein was limited in cultures containing pectin or cellulose as the primary carbon source, or in cultures lacking sucrose or casamino acids. Water-soluble extracts from coca stems inhibited production of the 24-kDa protein, whereas cellulose and pectin did not. Components produced by the plant may limit production of the 24-kDa protein in infected plant tissue and thereby limit the response of the plant to the fungus. These results suggest the 24-kDa protein does not function in the symptomatic phase of the F. oxysporum f.sp. erythroxylicoca disease interaction.

  3. Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia

    Energy Technology Data Exchange (ETDEWEB)

    Cole, Jesse; Gieler, Brandon; Heisler, Devon; Palisoc, Maryknoll; Williams, Amanda; Dohnalkova, Alice; Ming, Hong; Yu, Tian T.; Dodsworth, Jeremy A.; Li, Wen J.; Hedlund, Brian P.

    2013-08-15

    Several closely-related, thermophilic, and cellulolytic bacterial strains, designated JKG1T, JKG2, JKG3, JKG4, and JKG5, were isolated from a cellulolytic enrichment (corn stover) incubated in the water column of Great Boiling Spring, NV. Strain JKG1T had cells of a diameter of 0.7 - 0.9 μm and length of ~2.0 μm that formed non-branched multicellular filaments reaching >300 μm. Spores were not formed and dense liquid cultures were red. The temperature range for growth was 45-65 °C, with an optimum of 55 °C. The pH range for growth was 5.6-9.0, with an optimum of 7.5. JKG1T grew as an aerobic heterotroph, utilizing glucose, sucrose, xylose, arabinose, cellobiose, carboxymethylcellulose, filter paper, microcrystalline cellulose, xylan, starch, casamino acids, tryptone, peptone, yeast extract, acetate, citrate, lactate, pyruvate, and glycerol as sole carbon sources, and was not observed to photosynthesize. The cells stained Gram-negative. Phylogenetic analysis using 16S rRNA gene sequences placed the new isolates in the class Chloroflexia, but distant from other cultivated members, with the highest sequence identity of 82.5% to Roseiflexus castenholzii. The major quinone was menaquinone-9; no ubiquinones were detected. The major cellular fatty acids (>5%) were C18:0, anteiso-C17:0, iso-C18:0, and iso-C17:0. C16:0, iso-C16:0, and C17:0. The peptidoglycan amino acids were alanine, ornithine, glutamic acid, serine, and asparagine. Whole-cell sugars included mannose, rhamnose, glucose, galactose, ribose, arabinose, and xylose. Morphological, phylogenetic, and chemotaxonomic results suggest that JKG1T is representative of a new lineage within the class Chloroflexia, which we propose to designate Kallotenue papyrolyticum gen. nov., sp. nov., Kallotenuaceae fam. nov., Kallotenuales ord. nov.

  4. Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathway

    Directory of Open Access Journals (Sweden)

    Kosalková Katarina

    2012-01-01

    Full Text Available Abstract Background The secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway. Results Using the secretion of bovine chymosin in Aspergillus awamori as a model, we found a drastic increase (40 to 80-fold in cells grown with casein or casein phosphopeptides (CPPs. CPPs are rich in phosphoserine, but phosphoserine itself did not increase the secretion of chymosin. The stimulatory effect is reduced about 50% using partially dephosphorylated casein and is not exerted by casamino acids. The phosphopeptides effect was not exerted at transcriptional level, but instead, it was clearly observed on the secretion of chymosin by immunodetection analysis. Proteomics studies revealed very interesting metabolic changes in response to phosphopeptides supplementation. The oxidative metabolism was reduced, since enzymes involved in fermentative processes were overrepresented. An oxygen-binding hemoglobin-like protein was overrepresented in the proteome following phosphopeptides addition. Most interestingly, the intracellular pre-protein enzymes, including pre-prochymosin, were depleted (most of them are underrepresented in the intracellular proteome after the addition of CPPs, whereas the extracellular mature form of several of these secretable proteins and cell-wall biosynthetic enzymes was greatly overrepresented in the secretome of phosphopeptides-supplemented cells. Another important 'moonlighting' protein (glyceraldehyde-3-phosphate dehydrogenase, which has been described to have vesicle fusogenic and cytoskeleton formation modulating activities, was clearly overrepresented in phosphopeptides-supplemented cells. Conclusions In summary, CPPs cause the reprogramming of cellular metabolism, which leads to massive secretion of extracellular proteins.

  5. Burkholderia mallei and Burkholderia pseudomallei cluster 1 type VI secretion system gene expression is negatively regulated by iron and zinc.

    Directory of Open Access Journals (Sweden)

    Mary N Burtnick

    Full Text Available Burkholderia mallei is a facultative intracellular pathogen that causes glanders in humans and animals. Previous studies have demonstrated that the cluster 1 type VI secretion system (T6SS-1 expressed by this organism is essential for virulence in hamsters and is positively regulated by the VirAG two-component system. Recently, we have shown that T6SS-1 gene expression is up-regulated following internalization of this pathogen into phagocytic cells and that this system promotes multinucleated giant cell formation in infected tissue culture monolayers. In the present study, we further investigated the complex regulation of this important virulence factor. To assess T6SS-1 expression, B. mallei strains were cultured in various media conditions and Hcp1 production was analyzed by Western immunoblotting. Transcript levels of several VirAG-regulated genes (bimA, tssA, hcp1 and tssM were also determined using quantitative real time PCR. Consistent with previous observations, T6SS-1 was not expressed during growth of B. mallei in rich media. Curiously, growth of the organism in minimal media (M9G or minimal media plus casamino acids (M9CG facilitated robust expression of T6SS-1 genes whereas growth in minimal media plus tryptone (M9TG did not. Investigation of this phenomenon confirmed a regulatory role for VirAG in this process. Additionally, T6SS-1 gene expression was significantly down-regulated by the addition of iron and zinc to M9CG. Other genes under the control of VirAG did not appear to be as tightly regulated by these divalent metals. Similar results were observed for B. pseudomallei, but not for B. thailandensis. Collectively, our findings indicate that in addition to being positively regulated by VirAG, B. mallei and B. pseudomallei T6SS-1 gene expression is negatively regulated by iron and zinc.

  6. Carbon and Manganese Cycling in the Columbia River's Estuarine Turbidity Maxima in the South Channel

    Science.gov (United States)

    Bräuer, S. L.; Kranzler, K.; Tebo, B. M.

    2007-12-01

    The Columbia River represents the largest input (60-90%) of fresh water to the California Current System, and provides a major source of dissolved manganese and nutrients to the coastal waters. Researchers have identified upper Estuarine Turbidity Maxima (ETM(s)) as hot spots for microbial activity, and it is here that extensive manganese cycling is thought to occur. Most probable number counts of microorganisms within the ETM have revealed that the cultivable numbers of manganese-oxidizing bacteria are not statistically significantly different than that of other heterotrophs when grown on defined media with simple carbon sources or low concentrations (0.05%) of casamino acids and were in the range of 103 - 104 cells per mL. Similar numbers of heterotrophs (9.3 X 103 cells/mL) were found using a nutrient-rich complex medium; however, the numbers of manganese-oxidizers were significantly lower (~13 cells/mL). Approximately 100 different manganese-oxidizing bacteria were isolated from different media and are being phylogenetically characterized. Measurements of dissolved, ascorbate-reducible and total Mn by inductively coupled plasma- optical emission spectroscopy revealed that concentrations of Mn are positively correlated with turbidity and thus are higher during an ETM event. In addition, dissolved, total, and ascorbate-reducible Mn were all negatively correlated with salinity, supporting the idea that the manganese originates in the river and is diluted by the seawater originating off the coast. Uptake of 14C-labeled bicarbonate in response to various electron donors (nitrite, ammonium, thiosulfate, or Mn(II)) was stimulated during an ETM event but not before or after, indicating that these electron donors may serve as potential energy sources for carbon fixation. Taken together, our results further demonstrate that ETMs are zones with high microbial activity and that the ETM microbial communities harbor the potential for carbon fixation even in the relatively

  7. Response surface methodology based optimization of β-glucosidase production from Pichia pastoris.

    Science.gov (United States)

    Batra, Jyoti; Beri, Dhananjay; Mishra, Saroj

    2014-01-01

    The thermotolerant yeast Pichia etchellsii produces multiple cell bound β-glucosidases that can be used for synthesis of important alkyl- and aryl-glucosides. Present work focuses on enhancement of β-glucosidase I (BGLI) production in Pichia pastoris. In the first step, one-factor-at-a-time experimentation was used to investigate the effect of aeration, antifoam addition, casamino acid addition, medium pH, methanol concentration, and mixed feed components on BGLI production. Among these, initial medium pH, methanol concentration, and mixed feed in the induction phase were found to affect BGLI production. A 3.3-fold improvement in β-glucosidase expression was obtained at pH 7.5 as compared to pH 6.0 on induction with 1 % methanol. Addition of sorbitol, a non-repressing substrate, led to further enhancement in β-glucosidase production by 1.4-fold at pH 7.5. These factors were optimized with response surface methodology using Box-Behnken design. Empirical model obtained was used to define the optimum "operating space" for fermentation which was a pH of 7.5, methanol concentration of 1.29 %, and sorbitol concentration of 1.28 %. Interaction of pH and sorbitol had maximum effect leading to the production of 4,400 IU/L. The conditions were validated in a 3-L bioreactor with accumulation of 88 g/L biomass and 2,560 IU/L β-glucosidase activity.

  8. Constraint-based modeling of heterologous pathways: application and experimental demonstration for overproduction of fatty acids in Escherichia coli.

    Science.gov (United States)

    Ip, Kuhn; Donoghue, Neil; Kim, Min Kyung; Lun, Desmond S

    2014-10-01

    Constraint-based modeling has been shown, in many instances, to be useful for metabolic engineering by allowing the prediction of the metabolic phenotype resulting from genetic manipulations. But the basic premise of constraint-based modeling-that of applying constraints to preclude certain behaviors-only makes sense for certain genetic manipulations (such as knockouts and knockdowns). In particular, when genes (such as those associated with a heterologous pathway) are introduced under artificial control, it is unclear how to predict the correct behavior. In this paper, we introduce a modeling method that we call proportional flux forcing (PFF) to model artificially induced enzymatic genes. The model modifications introduced by PFF can be transformed into a set of simple mass balance constraints, which allows computational methods for strain optimization based on flux balance analysis (FBA) to be utilized. We applied PFF to the metabolic engineering of Escherichia coli (E. coli) for free fatty acid (FFA) production-a metabolic engineering problem that has attracted significant attention because FFAs are a precursor to liquid transportation fuels such as biodiesel and biogasoline. We show that PFF used in conjunction with FBA-based computational strain optimization methods can yield non-obvious genetic manipulation strategies that significantly increase FFA production in E. coli. The two mutant strains constructed and successfully tested in this work had peak fatty acid (FA) yields of 0.050 g FA/g carbon source (17.4% theoretical yield) and 0.035 g FA/g carbon source (12.3% theoretical yield) when they were grown using a mixed carbon source of glucose and casamino acids in a ratio of 2-to-1. These yields represent increases of 5.4- and 3.8-fold, respectively, over the baseline strain.

  9. Selective utilization of exogenous amino acids by Dehalococcoides ethenogenes strain 195 and its effects on growth and dechlorination activity.

    Science.gov (United States)

    Zhuang, Wei-Qin; Yi, Shan; Feng, Xueyang; Zinder, Stephen H; Tang, Yinjie J; Alvarez-Cohen, Lisa

    2011-11-01

    Bacteria of the genus Dehalococcoides are important members of bioremediation communities because of their ability to detoxify chloroethenes to the benign end product ethene. Genome-enabled studies conducted with Dehalococcoides ethenogenes 195 have revealed that two ATP-binding cassette (ABC)-type amino acid transporters are expressed during its exponential growth stages. In light of previous findings that Casamino Acids enhanced its dechlorination activity, we hypothesized that strain 195 is capable of importing amino acids from its environment to facilitate dechlorination and growth. To test this hypothesis, we applied isotopomer-based dilution analysis with (13)C-labeled acetate to differentiate the amino acids that were taken up by strain 195 from those synthesized de novo and to determine the physiological changes caused by the significantly incorporated amino acids. Our results showed that glutamate/glutamine and aspartate/asparagine were almost exclusively synthesized by strain 195, even when provided in excess in the medium. In contrast, phenylalanine, isoleucine, leucine, and methionine were identified as the four most highly incorporated amino acids, at levels >30% of respective proteinogenic amino acids. When either phenylalanine or all four highly incorporated amino acids were added to the defined mineral medium, the growth rates, dechlorination activities, and yields of strain 195 were enhanced to levels similar to those observed with supplementation with 20 amino acids. However, genes for the putative ABC-type amino acids transporters and phenylalanine biosynthesis exhibited insignificant regulation in response to the imported amino acids. This study also demonstrates that using isotopomer-based metabolite analysis can be an efficient strategy for optimizing nutritional conditions for slow-growing microorganisms.

  10. Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway.

    Science.gov (United States)

    Wang, Rongliang; Li, Lulu; Zhang, Biao; Gao, Xiaolian; Wang, Dongmei; Hong, Jiong

    2013-08-01

    To improve the xylose fermentation ability of Kluyveromyces marxianus, a xylose assimilation pathway through xylose isomerase was constructed. The genes encoding xylose reductase (KmXyl1) and xylitol dehydrogenase (KmXyl2) were disrupted in K. marxianus YHJ010 and the resultant strain was named YRL002. A codon-optimized xylose isomerase gene from Orpinomyces was transformed into K. marxianus YRL002 and expressed under GAPDH promoter. The transformant was adapted in the SD medium containing 1 % casamino acid with 2 % xylose as sole carbon source. After 32 times of trans-inoculation, a strain named YRL005, which can grow at a specific growth rate of 0.137/h with xylose as carbon source, was obtained. K. marxianus YRL005 could ferment 30.15 g/l of xylose and produce 11.52 g/l ethanol with a yield of 0.38 g/g, production rate of 0.069 g/l/h at 42 °C, and also could ferment 16.60 g/l xylose to produce 5.21 g/l ethanol with a yield of 0.31 g/g, and production rate of 0.054 g/l h at 45 °C. Co-fermentation with 2 % glucose could not improve the amount and yield of ethanol fermented from xylose obviously, but it could improve the production rate. Furthermore, K. marxianus YRL005 can ferment with the corn cob hydrolysate, which contained 20.04 g/l xylose to produce 8.25 g/l ethanol. It is a good platform to construct thermo-tolerant xylose fermentation yeast.

  11. Deferrisoma paleochoriense sp. nov., a thermophilic, iron(III)-reducing bacterium from a shallow-water hydrothermal vent in the Mediterranean Sea

    Science.gov (United States)

    Perez-Rodriguez, Ileana M.; Rawls, Matthew; Coykendall, Dolly K.; Foustoukos, Dionysis I.

    2016-01-01

    A novel thermophilic, anaerobic, mixotrophic bacterium, designated strain MAG-PB1T, was isolated from a shallow-water hydrothermal vent system in Palaeochori Bay off the coast of the island of Milos, Greece. The cells were Gram-negative, rugose, short rods, approximately 1.0 μm long and 0.5 μm wide. Strain MAG-PB1T grew at 30–70 °C (optimum 60 °C), 0–50 g NaCl l− 1 (optimum 15–20 g l− 1) and pH 5.5–8.0 (optimum pH 6.0). Generation time under optimal conditions was 2.5 h. Optimal growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Fe(III), Mn(IV), arsenate and selenate were used as electron acceptors. Peptone, tryptone, Casamino acids, sucrose, yeast extract, d-fructose, α-d-glucose and ( − )-d-arabinose also served as electron donors. No growth occurred in the presence of lactate or formate. The G+C content of the genomic DNA was 66.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Deferrisoma camini, the first species of a recently described genus in the Deltaproteobacteria. Based on the 16S rRNA gene phylogenetic analysis and on physiological, biochemical and structural characteristics, the strain was found to represent a novel species, for which the name Deferrisoma palaeochoriense sp. nov. is proposed. The type strain is MAG-PB1T ( = JCM 30394T = DSM 29363T). 

  12. Evaluation of supplementation of sucrose medium on the synthesis of Zymomonas mobilis bio-products - doi: 10.4025/actascibiolsci.v32i3.1519

    Directory of Open Access Journals (Sweden)

    Maria Antonia Pedrini Colabone Celligoi

    2010-09-01

    Full Text Available The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell

  13. Division of focal plane polarimeter-based 3 × 4 Mueller matrix microscope: a potential tool for quick diagnosis of human carcinoma tissues

    Science.gov (United States)

    Chang, Jintao; He, Honghui; Wang, Ye; Huang, Yi; Li, Xianpeng; He, Chao; Liao, Ran; Zeng, Nan; Liu, Shaoxiong; Ma, Hui

    2016-05-01

    A polarization microscope is a useful tool to reveal the optical anisotropic nature of a specimen and can provide abundant microstructural information about samples. We present a division of focal plane (DoFP) polarimeter-based polarization microscope capable of simultaneously measuring both the Stokes vector and the 3×4 Mueller matrix with an optimal polarization illumination scheme. The Mueller matrix images of unstained human carcinoma tissue slices show that the m24 and m34 elements can provide important information for pathological observations. The characteristic features of the m24 and m34 elements can be enhanced by polarization staining under illumination by a circularly polarized light. Hence, combined with a graphics processing unit acceleration algorithm, the DoFP polarization microscope is capable of real-time polarization imaging for potential quick clinical diagnoses of both standard and frozen slices of human carcinoma tissues.

  14. Two Papers on Majority Rule: Continuity Properties of Majority Rule with Intermediate Preferences. Electoral Outcomes with Probabilistic Voting and Nash Social Welfare Maxima.

    Science.gov (United States)

    1981-02-01

    1977], 310-334. Plott, C., "A Notion of Equilibrium and its Possibility Under Majority Rule," American Economic Review , 57 [19671, 787-806. Riker, W...1973], 553-560. Intriligator, M., &#34Income Redistribution: A Probabilistic Approach," American Economic Review , 69 [1979], 97-105. Kaneko, M., and K...Philadelphia: Saunders [1968). Plott, C., "A Notion of Equilibrium and its Possibility under Majority Rule," American Economic Review , 57 [1967], 787-806. Ray

  15. Effect of Variances and Manufacturing Tolerances on the Design Strength and Life of Mechanically Fastened Composite Joints

    Science.gov (United States)

    1978-12-01

    AD-A041/70 ,4 P𔄁operty of US Air .For, AAIWZ L1 brary AFFDLTR 78-179 ’Wrlght.Peatt Orson AF’B, EFFECT OF VARIANCES AND MANUFACTURING TOLERANCES ON...Degradation For Advanced Composites", Lockheed-California F33615-77-C-3084, Quar- terlies 1977 to Present. Phillips, D. C. and Scott , J. M., &#34The Shear

  16. Geophysical Tests for Intermediate-Range Forces

    Science.gov (United States)

    1993-11-01

    57. Basgall M., &#34Tower study hints at a ’sixth force"’, 3011-3019. Topics: F1,T, Science, 1987, 238. No. 4834, 1654-1655. Topics: 44. Barraco D. E...90, 91 123,212,214, 704, 738 Barr S. M., 41,42,43,327 Bracci L., 67,68 Cheung C. Y., 124 Barraco D. E., 44 Braginskil V. B., 92, 93, 94 Chin T.-S

  17. Better Management of Private Pension Plan Data Can Reduce Costs and Improve ERISA Administration.

    Science.gov (United States)

    1981-10-19

    benefit payable to any partlcioe nt to be o rsd" . . U.Sl. M091 I’M&#34W~IS Ms 31-0nosarl is51632 67 APPENDIX V APPENDIX V 5500-K Annual Return/Report of...c) 0 Multiemployer plan (d) [] Multipla -emplayer-collectively-bargained plan (e) E] Multiple-employer plan (other) 5 (a) (I) Mam of plan 10

  18. Department of Defense Doctrine Should Incorporate Sixty Years of Disaster Research in Order to Realistically Plan and Effectively Execute Disaster Response

    Science.gov (United States)

    2013-05-01

    people can only act out of respect for the moral law when they have a duty to do so.23 Utilitarianism or consequentialism Utilitarianism is an ethics ...Strosberg M, &#34Triage and Equality: An Historical Reassessment of Utilitarian Analyses of Triage." Kennedy Institute of Ethics Journal 2, no. 2 (1992...Robert and Martin Strosberg. "Triage and Equality: An Historical Reassessment of Utilitarian Analyses of Triage." Kennedy Institute of Ethics Journal 2

  19. Research and Development of Methods for Estimating Physicochemical Properties of Organic Compounds of Environmental Concern. Part 1.

    Science.gov (United States)

    1981-06-01

    Inc 4-32 i ____________________________ 42. Stevenson , P.M., &#34Organic Matter Reactions Involving Pesticides in Soil," in Bound and Conjugated Peeticide...organic compounds includes such naturally occur- ring substances as humus and lignin, as well as such anthropogenic substances as some of the...microbiology. Wood, E.J.F. [170] - Textbook on marine and estuarine micro- biology. Rodina, R.G. (138] - Textbook on aquatic microbiology. Stevenson , L.H. and

  20. Isothiazolone Volatility Study of a Water Bottom from Fuel Treated with Kathon(trade name) FP1.5 Biocide

    Science.gov (United States)

    1993-11-01

    specific biocides was evaluated. Pure cultures of Hormonoconis resinae (previously Cladosporium resinae ), Yarrowia lipolytica and Pseudomonas...R.A., "Growth of Cladosporium resinae in Sea Water/Fuel Systems", Developments in Industrial Microbiology, 22, 781 (1981). 5 MacGregor C. and Devitt S...Contractor Report CR/87/431, (1987). 8 Hebda A.J. and Jones G.M., &#34Conditions and Modifications to Conditions for Growth of C. resinae in Canadian

  1. Fundamental Mechanisms Affecting Friction Welding under Vacuum

    Science.gov (United States)

    1991-06-01

    z Professor Koichi Masubuchi Ocean Engineering Dept., Thesis Supervisor ~Certified by - CProfessor Ltmest Rabinowicz Mechanical Engineering Dept...welding and oxide layer affects. 60 REFERENCES 1. Rabinowicz ,E., "Friction and Wear of Materials", Wiley, 1964 2. SmithM., &#34Effect of Vacuum on the...Professor ELnest Rabinowicz Mechanical Engineering Dept., Thesis Reader Accepted by- 14,~/G 1, ~ Z a- ’A. Douglas Carn-chtir,-hirman Departmental Graduate

  2. Analytical Shear Buckling Investigation of Curved Composite Panels.

    Science.gov (United States)

    1984-12-01

    Technology Air University in Partial Fulfillment of the Requirements for the Degree of Masters of Science by - Jay K. McDaniel Captian, USAF Graduate...throughout this thesis. I would also like to thank the Air Force Flight Dynamics Laboratory, in particular Dr. N. S. Khot and Mrs. Elizabeth Copenhaver , for... Science Series, Volume IV), Stamford: Technomic Publishing Company, 1970. 13. Whitney, James M., &#34Buckling of Anisotropic Laminated Cylindrical Plates

  3. The Cholinergic Synapse International Symposium Held in Berlin, Germany on 23-27 September 1990

    Science.gov (United States)

    1990-09-27

    spinal cord. -R The PCR wait timed with degenerate oligonucleotide-primeru to amplify m 34 nucleotid" segment from chick brain poly A’ mAMA that encodes 11...BCHE) has been found in DNA from individuals with the "atypical" BCHE phenotype (McGuire et al., 1989) and from glioblastoma and neuroblastoma tumors ...question, the appearance of the mutation causing the Asp70 --> Gly substitution was pursued in DNA samples from various tumors and blood cell disorders

  4. Telephone Switching Career Ladder. AFSC 362X1

    Science.gov (United States)

    1990-03-01

    Emphasis and Task Difficulty Data .......... 26 Specialty Training Standard (STS) .... ............... 26 ELECTRONICS PRINCIPLES . . . . . . . . . . . . . . . . . . . . . . . . 3...EUE Ne V) <In -4- C )CA 00CLF CArnC~ C-4 (iCV C) lA < S- m &#34C4 c\\JC\\"CD -l(%,--4 it~ U-- L Lj- ULLi- LUU..W L 33 TABLE 19 ELECTRONICS PRINCIPLES USED BY

  5. An Analysis of Linear and Non-Linear Coherent Detection in Atmospheric Noise at Very Low Frequency

    Science.gov (United States)

    1974-11-01

    Bestimmte Integrale (Integral Table, Second Part , Definite Integrals), Fourth Edition, Springer-Verlag, Vienna (Austria), 1966. 20. Hall, Harry M., &#34A New...close agreement with the computational results of the analyses. The first part of the analysis is a survey of atmospheric noise representations. This...derived error rate perfomance data is presented which shows close agreement with the computational results of the analyses. The first part of the

  6. Power Management of Small Naval Vessels

    Science.gov (United States)

    2011-08-31

    Reference [16] and [6] explains the current differential scheme employed in SPS and HVDC systems respectively. Other schemes presently employed in DC...2002.985003 [3] Jiebei Zhu; Booth, C;, "Future multi-terminal HVDC transmission systems using Voltage source converters," Universities Power Engineering...Delivery, IEEE Transactions on , vol.25, no.4, pp.2308-2318, Oct. 2010 doi: 10.1109/TPWRD.2010.2044813 [6] Naidoo, D.; Ijumba, N.M.;, &#34 HVDC line

  7. The Shock and Vibration Digest. Volume 16, Number 8

    Science.gov (United States)

    1984-08-01

    have been examined [57]. the problem of shallow shells by using the Ritz method with algebraic polynomial trial functions Nonlinear analysi. A number...investigate the motion. Nonlinear differential equations have been vibrational behavior of turbine blades [42]. Experi- converted into nonlinear algebraic ...22 (4), Mech., Trans. ASME, 48 (3), pp 559-562 pp 247-264 (1980). (1981). 120. Tadws, R.N. and Rotman , M., &#34Dynamic Re- 130. Genta, G., Gola, M., et

  8. Kaumalapau Harbor, Hawaii, Breakwater Repair

    Science.gov (United States)

    2012-05-01

    and used to generate vertical tide corrections. RTK corrections were also fed to GPS units of the Coda F180 Motion Reference Unit ( MRU ) for horizontal...ISalc - L.SSurvry r r!M&#34 r.llll’l r.:::;! ~ ~c >HEET Al a~’ ERDC/CHL TR-12-7 129 9.2.3 Motion reference unit The Coda-Octopus F-180 MRU was

  9. An Analysis of the Price Analyst’s Role in the Source Selection Process

    Science.gov (United States)

    1991-09-01

    9:102) Ellram states that important criteria which should be included in any decision regarding selection of a supplier are the quantifiable...Commerce Clearing House, Inc., 1989. 16. Ellram , Lisa M. &#34The Supplier Selection Decision in Strategic Partnerships," Journal of Purchasing and...the solicitation, evaluation, and the selection decision ; (c) Ensure impartial and comprehensive evaluation of the offerors’ proposals; and (d) Ensure

  10. The Feasibility of Long-Term Contracting in the Department of Defense

    Science.gov (United States)

    1993-06-01

    Japan," INTERFACES, September-October 1992. 8. Ellram , Lisa, M., &#34The Supplier Selection Decision in Strategic Partnerships," Journal of Purchasing...sound purchasing decision under a best value approach. This is because long-term contracting arrangements encourage suppliers to put greater emphasis on...organizations with a MRP system, normally establish annual or long-term contracts with a few selected suppliers --and then place an order against the

  11. USSR Report, Physics and Mathematics

    Science.gov (United States)

    2007-11-02

    interferograms of the interaction regions were registered in argon, nitrogen and carbon dioxide , which were used to model different depths of physical... carbon dioxide gas. Output power of 45W was obtained, with gain of the active medium of 0.9 m&#34 • The output power per unit length of discharge is as...Frequency Passive Mode Locking in Iodine Photodissociation CV, M. Kiselev, A. S. Grenishin, et al.; KVANTOVAYA ELEKTRONIKA, No 8, Aug 84) 61 Long

  12. Recommended Best Industrial Practice for Software Architecture Evaluation.

    Science.gov (United States)

    2007-11-02

    openly gay , lesbian and bisexual students from receiving ROTC scholarships or serving in the military. Nevertheless, all ROTC classes at Carnegie Mellon...makes assumptions about patterns of resource utilization. How valid are these assumptions? Coupling and cohesion metrics make assumptions about the...Abowd, G.; & Webb, M. &#34SAAM: A Method for Analyzing the Properties Software Architectures." Proceedings of ICSE 16, Sorrento, Italy . (May 1994

  13. Dynamic Reconfiguration and Link Fault Tolerance in a Transputer Network

    Science.gov (United States)

    1989-06-01

    Naval Postgraduate School Monterey, CA 93943 5. Dr. Gregory Buzzard, Code 52Bu Department of Computer Sceince Naval Postgraduate School Monterey, CA...of MASTER OF SCIENCE IN COMPUTER SCIENCE from the NAVAL POSTGRADUATE SCHOOL June 1989 Author: Winfred P. Pikelis Approved by: / ’ 4-- Uno /R. Kodres...Th is Advisor ,\\ g ory fu. zlrd,. Second Reader Robert B. McGhee, Chairman, Department of Computer Science Kneale T. M&#34"Mt’l z -- Dean of Information

  14. JPRS Report Science & Technology USSR: Life Sciences.

    Science.gov (United States)

    1990-07-09

    Traumatology, Orthopedy and Field Surgery (head—professor M. F. Durov) and Chair of VK and LFK (head— docent P. G. Koynosov) of Tyumen Medical...Yu. D., Los, I. P and Popovich, V. M., &#34Fiziko-matematicheskaya problema deystviya elektro- magnitnykh poley i ionizatsii vozdukha" [The Physico...Article by S. I. Leonovich, docent , and Yu. M. Gain, candidate of medical science, Minsk] [Abstract] An international symposium on lasers in surgery

  15. A Composite Algorithm for Mixed Integer Constrained Nonlinear Optimization.

    Science.gov (United States)

    1980-01-01

    algorithm (FLEX) developed by Paviani and Himmelblau [53] is a direct search algorithm for constrained, nonlinear problems. It uses a variation on the...given in an appendix to Himmelblau [32]. Two changes were made to the program as listed in the rcference. Between card number 1340 and 1350 the...1972, pp. 293-308 (32] Himmelblau , D. M., Applied Nonlinear Programming, McGraw-Hill, 1972 (33] Himmelblau , D. M., &#34A Uniform Evaluation of Unconstrained

  16. Cost Effective and Affordable Guidance and Control Systems.

    Science.gov (United States)

    1985-02-01

    terminal areas’. EUROCONTROL Report 832004, March 1983. 4. Imbert, N., Fossard, A.J. at Comes, M. &#34 Gestion A moyen terme du traffic akrien en zone de...simultssnde de plusleurs fonictions opdrationnelles un "jugs do psix" systbme arbitre lea dvsntuels conflits et priorit6.- Contenu Ce document pr~sente dons...leo tiches suivantes - Is gestion do la mdmoire do visualisation - Joe tone tins graphiques dlimentaires. La gestion do Ia mdmnoire do visualisation

  17. Musculoskeletal and Medical Morbidity Associated with Rigorous Physical Training

    Science.gov (United States)

    1992-08-01

    displayed daily patient logs locally swimmer physical examination, and at the research facility to help ensure accuracy Prior to official training, all...Med. Vol. 3, No. 4, 1993 REPORT DOCUMENTATION PAGE __ ft....___ e’ Inqg amumm. • ue aMnWi•nbu e 4m neede mnTi@"Wd1 imt . m&#34il "ian ofi nimmim Sando misM

  18. The Determination of the Natural Frequencies and Mode Shapes for Anisotropic Laminated Plates Including the Effects of Shear Deformation and Rotatory Inertia.

    Science.gov (United States)

    1985-09-01

    shear effects began with Pryor and Barker [133. Their model was based on Reissner’s plate theory and was applied to the cylindrical bending of a...Theory. Englewood Cliffs: Prentice Hall, Inc., 1974. 6. Dym, Clive L. and Irving H. Shames. Solid Mechanics: A Variational Approach. New York: McGraw-Hill...13. Pryor, Jr., C. W. and Barker , R. M. &#34A Finite Element Analysis Including Transverse Shear Effects for Applications to Laminated Plates,’ AIAA J

  19. The Maritime Strategy Debates: A Guide to the Renaissance of U.S. Naval Strategic in the 1980s. Revision 2

    Science.gov (United States)

    1989-09-30

    Capt. Douglas M., &#34Responding to the Soviet Presence in Northern Waters: An American Naval View," in Archer, Clive (ed.), The Soviet Union and...a key element in carrying out the Maritime Strategy. Archer, Clive and Scrivener, David (eds), Northern Waters: Security and Resource Issues, Totowa...1897, especially pp 178-182 and 196-197); Barker , A., J., The War Against Russia, 1854-1856, New York: Holt, Rinehart and Winston, 1970; Curtiss

  20. Next-generation Surface Enhanced Raman Scattering (SERS) Substrates for Hazard Detection

    Science.gov (United States)

    2012-09-01

    Izake, E.L., " Forensic and homeland security applications of modern portable Raman spectroscopy," Forensic Science International, 1-8 (2010). [19...Yazici, M.M., Kahraman, M., Sahin, F., Gulluce, M., &#34Characterization of Thermophilic Bacteria Using Surface-Enhanced Raman Scattering," Applied...pathogenic bacteria ," Proceedings of the SPIE - The International Society for Optical Engineering, 73130K (73110 pp.) (2009). [69] Hankus, M.E

  1. Long-Term Changes in Beach Fauna at Duck, North Carolina.

    Science.gov (United States)

    1982-11-01

    and Gulf of Mexico coasts, while not diverse, has been remarkably successful in coping with unstable substrate caused by high kinetic wave energy...lecolepides vir-idis + + Laeonereio culveri + + Lyeippides grayi ++ Po Zydora ligni+ Oligochaeta + + Mollusca Ran gia cuneata + + Macomaz sp. + Phyea sp...Tropics, Vol. 22, 1974, pp. 51-66. DEXTER, D.M., &#34The Sandy-beach Fauna of Mexico ," Southwest, Nature, Vol. 20, 1976, pp. 479-485. DIAZ, H., "The Mole Crab

  2. A Medical Area Network of Virtual Technology (MANVT)

    Science.gov (United States)

    2011-10-01

    Zhang, Y., Hu, H., Shriver, C., Hooke, J., & Liebman, M. &#34 Caffeine Intake, Race, and Risk of Invasive Breast Cancer, Lessons Learned from Data...illustrated in Fig. 3, the model is composed of six major modules: Medical History, Physical Exam , Diagnostic, Treatment, Outcome, and Scheduling and...article.) 1010 H. Hu et al.j]oumal of Biomedical Informatics 44 (2011) 1004-1019 IE Medical History Ill Physical Exam 13 Diagnostic EJ Biopsy IE

  3. America, Linearly Cyclical

    Science.gov (United States)

    2013-05-10

    2013. Web. 23 Apr. 2013. Robinson, David M. &#34The Wheel of Fortune." Classical Philology 41.4 (1946): 207-216. JSTOR Web 20 Apr. 2013. Spalding...Matthew. "George Washington’s Farewell Address." The Wilson Quarterly 20.4 (1996): 65-71. JSTOR Web. 20 Apr. 2013. The Constitution. National Centerfor...Thomas Tanselle." The New England Quarterly 40.4 (1967): 580- 583. JSTOR Web 20 April2013

  4. Field Applicable Method to Reduce Dental Emergencies.

    Science.gov (United States)

    1982-04-15

    262-29t Salle. A.J.: Heavy metals other than mercury and (1977). silver; in Laurence , Block, Disinfection, sterili- flock, J.; Tinanoff, N.: Resolution...otisertatrsus Intl luhnit itrt t5tilrIll5.i rl-ifs m&#34f .srl𔃻i-t.’ sir arte contduicted hN an ri ditid ua affiliated with an rintirtuttin a hichi a rees

  5. Behavior of Partially Restrained Reinforced Concrete Slabs.

    Science.gov (United States)

    1986-09-01

    Takehira, Derecho , and Iqbal [34] followed a similar approach to arrive at recommended design criteria for 1 the Naval Facilities Engineering Command...Datta, T. K., "Ultimate Strength of Reinforced Concrete Slab-Beam Systems: A New Approach," r Indian Concrete Journal, Bombay, India , Vol. 47, 1973, pp...Journal of Solids and Structures, Vol. 1, 1965, pp. 97-111. 34. Takehira, T., Derecho , A.T., and Iqbal, M., &#34Design Criteria for Deflection Capacity

  6. Multivariate Modelling of the Career Intent of Air Force Personnel.

    Science.gov (United States)

    1980-09-01

    motivation, Victor H . Vroom developed in 1964 what is known today as the first integrated model of expectancy theory. Adding the historical perspective...LSSR 72-80. Vroom , Victor H . Work and Motivation. New York: Wiley, 1964. Vrooman, Roger M. &#34An Analysis of Factors Associated With the Job...results of the research may, in fact, be ip nt. Whether or not you were able to establish an equivalent value for h research (3 above), what is your

  7. Proceedings of the NRDEC (Natick RD&E Center) Science Symposium Held on 2-4 June 1986 in Natick, Massachusetts. Volume 1

    Science.gov (United States)

    1986-06-04

    December 1985) 3. Billmeyer , F. w., Saltzman , M. , &#34Principles of Color Technology", Interscience Publishers, New York, 1966 4. Judd, D.B...operate, and rugged for practical use in an industrial environment . Dr . Fred Billmeyer and Ms. Paula Alessi, Rensselear Cclor Measurement Laboratory...Commerford, T.R., Hepfinger, L.B., "Ob jective Color Measuring System," NRDEC Tech. Rep . No. NATICK/TR-83/005 (September 1982) 2. Billmeyer

  8. Fire Safety Aspects of Polymeric Materials. Volume 1. Materials. State of the Art

    Science.gov (United States)

    1977-01-01

    Encyclopedia (1973-1974) and in the textbooks by Billmeyer (1971) and Srydson (1975). The Polymer Handbook (Brandrup and Immergut, 1374) and the Handbook of...formulation variables. Their chemistry is too involved to be described in detail here. More information may be found in Bydsen (1975), Billmeyer ...1973). Bhatnagar, V. M., &#34Fire Retardant Formulations Handbook," Vol. 1, p. 1, Technomic PuW. Co., Inc., Westport, Conn. (1972). Billmeyer , F. W

  9. A Generalized Polynomial Form of the Objective Function in Flash Calculations

    Science.gov (United States)

    1992-05-01

    Redlich - Kwong Equation of State , Applications to Gen- eral Physical Data Calculations," paper 15C...G.M., &#34A Modified Redlich - Kwong Equation of State , Applications to Gen- eral Physical Data Calculations," paper 15C, Proceedings, AIChE National Meeting...acceptance are Soave’s modifications of the Redlich - Kwong (1949) equation of state (SRKEOS) (Soave, 1972) and that presented by Peng and Robinson

  10. Estimating Sea Surface Salinity in Coastal Waters of the Gulf of Mexico Using Visible Channels on SNPP-VIIRS

    Science.gov (United States)

    2014-01-01

    surface salinity estimates for the Northern Gulf of Mexico . In situ salinity data collected from six coastal moorings were compared to calibrated, geo...34Geographic and hydrodynamic characteristics of shallow coastal lagoons ," Marine Geology 88, 187-199 (1989) [3] Gordon, H.R., and Wang, M., &#34Retrieval...1. HEPORJ OI\\JE (DD-MM-YYYY) 30-07-2014 REPORT TYPE Conference Proceeding 4. TITLE AND SUBTITLE Estimating Sea Surface Salinity in Coastal

  11. The Slippery Slope of Air Force Downsizing: A Strategy Connection

    Science.gov (United States)

    2013-02-14

    Meinhart , Richard M. &#34Chapter 7 - National Military Strategies: 1990-2009." U.S. Army War College, n.d. National Security Strategy Archives. n.d...service can be seen in comparison. 3 Force structure follows military expenditures as associated with GDP. 11 Army and Marine Corps Force... Army or Marines, as resources become tighter, the Air Force will not have wartime increases from which to reduce. (see Figure 4-212). In FY2001 the

  12. The Need and Requirements for Validating Damage Detection Capability

    Science.gov (United States)

    2011-09-01

    Medina, E. A., Lindgren, E. A., Buynak, C. F., Steffes, G., Derriso , M., “Model-assisted Probabilistic Reliability Assessment for Structural Health...130 Aircraft”, Aircraft Airworthiness and Sustainment, (Austin, TX, 2010). 9. Lindgren, E. A., Buynak, C. F., Aldrin, J. C., Median, E. A., Derriso ...G., Derriso , M., &#34Model- assisted Probabilistic Reliability Assessment for Structural Health Monitoring Systems," Review of Progress in QNDE, Vol

  13. USSR Report, Electronics and Electrical Engineering, No. 111

    Science.gov (United States)

    2007-11-02

    Cinematography , and BONDARCHUK, 7. M,, IRZ, P. 7., GILINSKIY, A. G. and DROZDOV, 7. M., &#34Ekran" Scientific and Production Organization iAbstract] Major...dimensions and currents in the buses. The shielding factor representing the primary electromagnetic parameter acting on the bus of a given lead is...practically independent of the pattern of current change in the bus with time. The actual electrodynamic forces acting on the buses were calculated by a

  14. Selected Papers from the Conference on Computer Interfaces and Intermediaries for Information Retrieval (1st) Held in Williamsburg, Virginia on 3-6 October 1984,

    Science.gov (United States)

    1986-05-01

    Tamas; Rapp, Barbara A.; and Schoolman, Harold M. &#34Automated Information Retrieval in Science and Technology." Science 208:25-30; April 4, 1980. ° .. 52...be better. .2 77 I% o 4C.C H. C 3c.c 2f. C -, 2C.C1 BELL CAFFA CRUZ DORAN CARPER GRUBB VE KNIGHI PMA THC’N I AF 0- AP I OF 7 14 LECI. IL EI AWE, I-e

  15. Risk Management Concepts and Guidance

    Science.gov (United States)

    1989-03-01

    and Information Processing Under Various Uncertainty Conditions," Bowling Green State University. 81. DeGroot , M.H., Optimal Statisitical Decisions...Journal, Vol. 14, No. 4, 1978, pp. 12-17. 243. Morehouse, W., "Progress in Resources Planning Through PERT," General Electric Co.. 1960. 244. Morris , J.M...34, 1987, John Wiley & Sons Ltd. Atzinger, E.M. et al, Compendium on Risk Analysis Techniques, DARCOM Material Sys- DeGroot , M.H., "Optimal Statistical

  16. Recent Developments in the Identification of Mechanisms for Microbiologically Influenced Corrosion

    Science.gov (United States)

    2010-09-23

    31 (1997) pp.813-818. 36 Weiner, J.M., Lovley D.R.; "Rapid Benzene Degradation in Methanogenic Sediments from a Petroleum-Contaminated Aquifer ...wide variety of electron donors and acceptors. In the absence of an electron acceptor, SRB can enter into syntrophic relationships with methanogens or...Microorganisms," Corrosion, 64(2008) pp.854-859. 22 Dolfing, J., Tiedje J.M.; &#34Acetate Inhibition of Methanogenic Syntrophic Benzoate Degradation

  17. An Investigation of the High Rate Volumetric Properties of Snow.

    Science.gov (United States)

    1981-11-01

    1 o. t "r111’ us"’I by St .a~w’Iv~~ iadl . I u 1I ’I ’(1700: ~ ~ ~ ~ ~ ~ ~ ~ ~ a IW l’r-idi:f II a io t .i 1 lil J!’,U1 I ILI’’ intr11 1 i M&#34,0 1011

  18. Human Factors Engineering. Part 2. HEDGE (Human Factors Engineering Data Guide for Evaluation)

    Science.gov (United States)

    1983-11-30

    1.8.2h. pot ons. ’a t.’l’t "A1e *He;gttr:o-drb raboa 5.3; Ř" , I H-dl’t’~is teOnt’,oi by 11h% oe i’t" t’.t~t . - ott e saetdrbh der; M&#34&’&W’h

  19. Strategic Materials Industry Study. Industry Study, Spring 2008

    Science.gov (United States)

    2008-01-01

    Industrial College of the Armed Forces, Washington D.C.: March 3, 2008. Solow , Robert M. “Technical Change and the Aggregate Production Function...impossible.cfm. Bunting, Robert M. &#34Vanadium - Recent Developments in Supply and Demand." Orlando, Florida, October 9, 2007. Bureau of Labor...Strategic Materials?” Resource Investor. http://www.resourceinvestor.com/pebble.asp?relid=30401. Gilpin, Robert . Global Political Economy

  20. New Bright Optical Object on South Hemisphere detected by MASTER at the X-Ray source position.

    Science.gov (United States)

    Gagarin, Yu. A.; Korolev, S. P.; Lipunov, V.; Pogrosheva, T.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gress, O.; Kuznetsov, A.; Shumkov, V.; Popova, E.; Vladimirov, V.; Kornilov, V.; Gorbunov, I.; Krylov, A.; Chazov, V.; Podesta, R.; Levato, H.; Lopez, C.; Saffe, C.; Gabovich, A.; Rebolo, R.; Serra-Ricart, M.

    2017-04-01

    MASTER-OAFA auto-detection (Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) system discovered OT source at (RA, Dec) = 17h 45m 34.82s -64d 30m 44.4s with 0.7 arcsec error on 2017-04-12.25627 UT. OT unfiltered magnitude is 16.7m (mlimit=18.2m).

  1. Experimental Modal Analysis and Dynamic Component Synthesis. Volume 1. Summary of Technical Work

    Science.gov (United States)

    1987-12-01

    Rades. M., &#34Identification of the Dynamic Characteristics of a Simple System with Quadratic Damping," Serie de Mecanique Appliquee. 28 (4), 1983, pp...Measurement and Analysis Requirements," SAE Paper Number 751066 1975, 17 pp. [261 Prony, R. "Essai Experimental et Analytique sur les Lois de [a...of the Dynamic Characteristics of a Simple System with Quadratic Damping, Serie de Mecanique Appliquee, Vol. 28, Num. 4, 1983, pp. 439-446. 11391

  2. The Zapatista Social Netwar in Mexico

    Science.gov (United States)

    1998-01-01

    on the Internet. 4From a statement by Subcommandante Marcos, March 4, 1994, as reported by the Academia Mexicana de Derechos Humanos , Special...Indigenas de Mexico, Mexico City: Comisiön Nacional de Derechos Humanos , 1995. Evan, William M., &#34An Organization-Set Model of Interorganizational...City: Comision Nacional de Dere- chos Humanos , 1995. Meisel, James, The Fall of the Republic: Military Revolt in France, Ann Arbor: University of

  3. An Initial Assessment of Coal-Fired Ship Operations.

    Science.gov (United States)

    1984-11-01

    presence of oversized rock and clinkers * generated in the boiler. - Blockages in the ash transfer system. - Burning coal fed through the ash system. Many...close quarters. Engineers indicate that maintaining "engine r.p.m.&#34 within one revolution is necessary during canal transits . Propulsion Control...injection of exhaust gas into . ventilation inlets. Fresh air makeup for ventilation systems did not Initially .Its have filter elements . Filters have been

  4. The Shock and Vibration Digest. Volume 13. Number 7

    Science.gov (United States)

    1981-07-01

    predicted from first principles [70]. Grabec and Leskovar [71] showed that it is not practically feasible to use acoustic emission analysis to evaluate...145-150 (1976). 14. Peters , J. and Mergeay, M., &#34Dynamic Analysis of Machine Tool Using Complex Modal Method," Ann. CIRP, 25(1), pp 257-261 (1976...namics and Process Parameters," Intl. J. Mach. Tool. Des. Res., 16 (4), pp 301-318 (1976). 71. Grabec, I. and Leskovar ." Acoustic Emission of a

  5. Rochester Connectionist Papers. 1979-1985

    Science.gov (United States)

    1985-12-01

    updated and improved version of the thesis account of recent neurolinguistic data. Fanty, M., &#34Context-free parsing in connectionist networks." TR 174...April 1982. Our first large program in the connectionist paradigm. It simulates a multi- layer network for recognizing line drawings of Origami figures...The program successfully deals with noise and simple occlusion and the thesis incorporates many key ideas on designing and running large models. Small

  6. On Market Games,

    Science.gov (United States)

    1968-12-01

    n-Person Games, Ph.D. Thesis, Princeton University, June 1953. 5. Gillies, D. B., "Solutions to general non-zero-sum games," Annals of Mathematics Study...Economic Review). 22. Shubik, M., &#34Edgeworth market games, Annals of Mathematics Study 40 (1959), 267-278. 23. von Neumann, J., and 0. Morgenstern, Theory... of . Mathematics Study 40 (1959), 145-162; also The RAND Corporation, P-1392, June 1958. 16. , Values of Large Games - VII: A General Exchange Economv

  7. Retractor-Based Stroking Seat System and Energy-Absorbing Floor to Mitigate High Shock and Vertical Acceleration

    Science.gov (United States)

    2014-04-15

    III 50th Male Dummy Parts Catalogue, Rev. 4, July 2013 [8] Seat Belt Retractor Performance Evaluation in Rollover Crashes. Klima, M., Toomey, D., and...Weber, M., &#34 Seat Belt Retractor Performance Evaluation in Rollover Crashes," SAE Technical Paper 2005-01-1702, 2005, doi:10.4271/2005-01-1702 [9...restrained with a standard automotive seat belt system. A retractor-based seat belt system is attached to the seat structure with varying load

  8. Critical Review and Technology Assessments, 󈨟-󈨠

    Science.gov (United States)

    1991-09-01

    Techniques Push the High Temperature Frontier," Microwave Journal, 1988. 87. Priore, M., &#34Discrete Semiconductor Device Reliability," RAC Publication, DSR- 4...ENVIRONMENTALLY OOBLISHB.BE. HONEYWELLINC. PREICTIVE 24867-011 PROTECTEDITAPE-AUTOMATED-BONDED BAKKES.J. TECNOLOGY INTEGRATED CIRCUITS ARNO.R.G... PUSHING TEE PENTAGON TOWARD QML ANON. NONE MILITARY & AEROSPACE 24321.000 ELECTRONICS. Pages 39.40 FIRST SILICON. THEN GALLIUM ARSENIDE. THEN

  9. A Review of Optically Controlled Microwave Devices and Circuits

    Science.gov (United States)

    1992-09-01

    Japanese Journal of Applied Physics , Vol. 19, No. 1, pp. L27-29, January 1980. 26. Gammel, J. C., Ballantyne, J. M...34Comments on ’High Speed Photoresponse Mechanism of a GaAs MESFET’," Japanese Journal of Applied Physics , Vol. 19, No. 5, pp. L273-275, May 1980. 27...818, June 1987, Las Vegas, Nv. 55. Umeda, T., Cho, Y., Shibatomi, A., "Picosecond HEMT Photodetector," Japanese Journal of Applied Physics

  10. Long Term Statistical Measurements of Environmental Acoustics Parameters in the Arctic. AEAS Report Number 2. Low Frequency Transmission Loss Measurements in the Central Arctic Ocean.

    Science.gov (United States)

    2014-09-26

    34 . ...................... ..-.. . - - - - - -- -4-4 4 - -*-.. - -w c- t ~ - . -. a m. -os - 7 U., *. 1 Lol a, r. m &#34 III I Ic ~II a to ml -7 lit C 10 0 n 0 %0 (0) cn~ * ’" S 070 LLA LA L _ _ _j_...r- %a - --- .. ..... ... . ~ . ~ . . . qf Lf ) ) U)U 0 7 LOl -J Cm-0m(oPs Zbenos rl- * I-) _ _ _ _ __ _ _ __S

  11. Rotationally Symmetric Operators for Surface Interpolation

    Science.gov (United States)

    1981-11-01

    Uzawa H. Studies in linear and nonlinear programming, Stanford University press, Stanford, 1958. 131 Barrow H. G. and Tenenbaum J. M. &#34Interpreting...Nonsense Sentences," Machine Intelligence 6, eds. Meltzer B. and Michie D., Edinburgh University Press, 1971. [19] Ikeuchi K. and Horn B. K. P. "Numerical...an object from a single view," Artificial Intelligence 17 (1981), 409-460. [21] Knuth 1). E. "Mathematical typography ," Bull. Amer. Math. Soc. (new

  12. A State-of-the-Art Assessment of Automatic Name Placement.

    Science.gov (United States)

    1986-08-01

    NAME PLACEMENT 4. Hirsch, S.A., Algorithms for automatic name placement of point data, Mater’s thesis, Department of Geography, State University of New...maps, Master’s Project, Department of Geography, State University of New York, Buffalo, NY, December 1980. 79p. plus program listing. This master’s...develop an automatic name placement system. 11 Balodis, M., &#34Positioning of typography on maps," Proc. ACSM Pall Con- vention, Salt Lake City, Utah, Sept

  13. Strong earthquake activity all over the world and strong-moderate earthquake activity within and near China (June, 2000~ Jnly, 2000)

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@ Illustration All the data in this catalog are chosen from the 2Preliminary Seismological Report of Chinese Seismic Stations2 (Its abbreviation is 2Monthly Report2). The catalog includes the events of M34.7 in and near China and M36 all over the world. The 2Monthly Report2 is monthly compiled by the Ninth Section of Institute of Geophysics, CSB.

  14. Heat Stress Evaluation of Anti-Exposure Flight Gear

    Science.gov (United States)

    1985-05-15

    Durnin. J.V.G.A. and Rahaman. M.M. &#34The Assessment of the Amount of Fat in the Human Body from Measurements of Skinfold Thickness :: Br. J. Nutrit...1X230M4 ".;nc~aS.f.# NADC-85061-60 TABLE OF CONTENTS Page LIST OF TABLES ................................................. LIST OF FIGURES...85061-60 LIST OF TABLES " Table Page 1 Test Subject Profiles .......................................... 3Ŗ Flightgear W orn, All Configurations

  15. The Relationship of Temperature to Strength and Power Production in Intact Human Skeletal Muscle.

    Science.gov (United States)

    1979-06-01

    graphically depicts the relationship of thigh skin- fold to Tm changes. It can be seen from this figure that the thickness of the thigh skinfold did not...this a reality instead of a dream. - t ’ I L -iv , -_ I I I il m--&#34 " ll Tm l ....... TABLE OF CONTENTS K i Page LIST OF TABLES ...COMMITTEE APPROVAL LETTER .. ............ 48 APPENDIX C: CALIBRATION CURVE FOR NEEDLE TEMPERATURE PROBE. ................. 50 iv I TABLE OF CONTENTS

  16. Sensor Validation Using Nonlinear Minor Component Analysis (Preprint)

    Science.gov (United States)

    2006-05-01

    IEEE Transactions on Automatic Control , vol. 47, no. 4, pp. 576-593, April...based approach ", IEEE Transactions on Automatic Control , vol. 49, no. 8, pp. 1259-1274, August 2004. 7. Zhang, X., Parisini, T., and Polycarpou, M. M...34A sensor bias fault isolation scheme for a class of nonlinear systems", Accepted for publication in IEEE Transactions on Automatic Control

  17. Robust Control for Linear Systems with Structured Uncertainty

    Science.gov (United States)

    1988-11-01

    IEEE Transactions on Automatic Control , Vol. AC-29, no. 10, pp. 935-936, 1984. 4. Bose, N. K., "A...1985. 5. Anderson, B. D. 0., Jury, E. I., and Mansour, M., &#34On Robust Hurwitz Polynomials", IEEE Transactions on Automatic Control , Vol. AC-32, pp. 809...B., Berger, C. S., and Dabke, K. P., "On Stability of Polynomials with Perturbed Coefficients", IEEE Transactions on Automatic Control , Vol. AC-30,

  18. Remedial Investigation Badger Army Ammunition Plant, Baraboo, Wisconsin. Volume 2. Appendices D.2 Through F

    Science.gov (United States)

    1991-01-01

    Well Number DNK Wed Numoer TypeofWell WafTableOtMrvanonWeHlElU LAeca Locauon Dat Wenl Instaled Pistanc Wel Is N/4 of l/4 of Seco _.._.., m -&#34 V v D. R...Perons tamne Firm) Distance wellIs Frina WastedSource boundary Tel Intale Rr - Peso W N!T p.d / Is We.I A Point or Encorcemam StUL Application! a U cim

  19. Effects of cryoprotectants on the viability and activity of freeze dried recombinant yeasts as novel oral drug delivery systems assessed by an artificial digestive system.

    Science.gov (United States)

    Blanquet, Stéphanie; Garrait, Ghislain; Beyssac, Erick; Perrier, Céline; Denis, Sylvain; Hébrard, Géraldine; Alric, Monique

    2005-09-01

    The aim of this study was to investigate, in a gastric-small intestinal system TIM-1, the effect of cryoprotectants on the survival of freeze-dried Saccharomyces cerevisiae expressing the heterologous P450 73A1 and their ability to convert trans-cinnamic acid into p-coumaric acid. Yeasts were lyophilized in suspensions of trehalose, maltose, lactose, or a milk proteins/trehalose mix. Freeze-dried or native yeasts and trans-cinnamic acid were introduced simultaneously into TIM-1 at the beginning of digestion. Yeast survival rate was evaluated by cell counting in the ileal effluents. P450 73A1 activity was followed by HPLC assay of p-coumaric acid. Freeze-dried yeasts showed high tolerance to digestive conditions. Nevertheless, their survival rate was lower than that of non-dried cells (around 80% whatever the protective agent vs. 96%). The ability of recombinant freeze-dried S. cerevisiae to perform a bioconversion reaction in the digestive tract was shown with all the protectants. The highest trans-cinnamic acid conversion rate (24 vs. 41% for native yeasts) was obtained with the milk proteins/trehalose mix. These results show that freeze-drying might be considered for the pharmaceutical formulation of new drug delivery systems based on orally administered recombinant yeasts and that TIM-1 could be a helpful tool for the pre-screening of oral dosage forms.

  20. Specific phenolic compounds and sensory properties of a new dealcoholized red wine with pomegranate (Punica granatum L.) extract.

    Science.gov (United States)

    Tárrega, Maria Amparo; Varela, Paula; Fromentin, Emilie; Feuillère, Nicolas; Issaly, Nicolas; Roller, Marc; Sanz-Buenhombre, Marisa; Villanueva, Sonia; Moro, Carlos; Guadarrama, Alberto; Fiszman, Susana

    2014-09-01

    The pomegranate (Punica granatum L.) fruit has a long history of human consumption and possesses notable antioxidant and cardiovascular properties. This work evaluated the feasibility to provide a new functional beverage based on a dealcoholized red wine matrix supplemented by a pomegranate extract. The potential bioactive compounds in the pomegranate extract, punicalagin A and B and ellagic acid, were analyzed during the downstream process in order to evaluate the functional dose in the final beverage. The addition of pomegranate extract to the dealcoholized red wine resulted in a product with more intense yeast odor, acidity, yeast flavor, and astringency and with a less intense berry flavor. Consumer acceptance of the product was also investigated and the results revealed the existence of a niche of consumers willing to consume dealcoholized wine enriched with pomegranate extract. After tasting, 50% and 40% of those consumers initially interested by this product concept declared to be interested to purchase the control sample and the functional beverage, respectively. The daily consumption of two servings of 250 mL of this new pomegranate-enriched dealcoholized wine provides 82 mg of total ellagitannins, corresponding to the sum of punicalagin A and B and ellagic acid.

  1. Yeast prions: structure, biology, and prion-handling systems.

    Science.gov (United States)

    Wickner, Reed B; Shewmaker, Frank P; Bateman, David A; Edskes, Herman K; Gorkovskiy, Anton; Dayani, Yaron; Bezsonov, Evgeny E

    2015-03-01

    A prion is an infectious protein horizontally transmitting a disease or trait without a required nucleic acid. Yeast and fungal prions are nonchromosomal genes composed of protein, generally an altered form of a protein that catalyzes the same alteration of the protein. Yeast prions are thus transmitted both vertically (as genes composed of protein) and horizontally (as infectious proteins, or prions). Formation of amyloids (linear ordered β-sheet-rich protein aggregates with β-strands perpendicular to the long axis of the filament) underlies most yeast and fungal prions, and a single prion protein can have any of several distinct self-propagating amyloid forms with different biological properties (prion variants). Here we review the mechanism of faithful templating of protein conformation, the biological roles of these prions, and their interactions with cellular chaperones, the Btn2 and Cur1 aggregate-handling systems, and other cellular factors governing prion generation and propagation. Human amyloidoses include the PrP-based prion conditions and many other, more common amyloid-based diseases, several of which show prion-like features. Yeast prions increasingly are serving as models for the understanding and treatment of many mammalian amyloidoses. Patients with different clinical pictures of the same amyloidosis may be the equivalent of yeasts with different prion variants.

  2. Lipase production in lipolytic yeast from Wonorejo mangrove area

    Science.gov (United States)

    Alami, Nur Hidayatul; Nasihah, Liziyatin; Umar, Rurin Luswidya Artaty; Kuswytasari, Nengah Dwianita; Zulaika, Enny; Shovitri, Maya

    2017-06-01

    Lipase is an enzyme that is often used in industry and become a commercial enzyme. One group of microorganisms capable of producing lipase is a yeast. This study aims to screen yeast from Wonorejo mangrove that potential to produce lipase and to optimize the production of these enzymes. Screening test include the measurement of lipolytic index and value of fatty acid. Yeast with the best value of fatty acid will be continued to the measurement of lipase activity. It is affected by several environmental factors, such as pH, temperature, and incubation time. This research was conducted to observe the optimization variation on environmental factors combination to produce lipase. Lipase activity was tested by using p-Nitrophenyl Palmitate (pNPP). Absorbency was measured by spectrofotometer on wavelength of 410 nm. Measurement of the enzyme activity was done by interpolating the absorbance values on the p-nitrophenol standard curve then calculated by the formula. All data were analyzed by using descriptive quantitative method. The results show that the highest lypolityc index was 2.08. The highest value of fatty acid was 0.49 that was reached on 168 hours of incubation. Candida W3.8 expressed the highest lypolylitic potential. The optimum environment to produce lipase by Candida W 3.8 was on 120 hours of incubation time, in temperature range of 27°C - 45°C and pH range of 4,5 - 7.

  3. Larval starvation reduces responsiveness to feeding stimuli and does not affect feeding preferences in a butterfly.

    Science.gov (United States)

    Kehl, Tobias; Fischer, Klaus

    2012-07-01

    It is commonly assumed that holometabolic insects such as Lepidoptera rely primarily on larval storage reserves for reproduction. Recent studies though have documented a prominent role of adult-derived carbohydrates for butterfly reproduction. Moreover, a few studies have shown that adult butterflies may also benefit from adult-derived amino acids, at least when larval storage reserves are reduced. Given that in holometabolous insects larval deficiencies are carried over into the adult stage, reduced storage reserves have the potential to modulate adult feeding preferences and responses in order to allow for a successful compensation. We tested this hypothesis here in the fruit-feeding butterfly Bicyclus anynana using larval food stress to manipulate storage reserves. Alcohols (methanol, ethanol, butanol, propanol), sugars (maltose, glucose, fructose, sucrose), and acetic acid acted as feeding stimuli, while butterflies did not respond to other substances such as amino acids, yeast, salts, or vitamins. Contrary to expectations, stressed butterflies showed a weaker response than controls to several feeding stimuli. In preference tests, butterflies preferred sugar solutions containing proline, arginine, glutamic acid, acetic acid, or ethanol over plain sugar solutions, but discriminated against salts. However, there were no general differences among starved and control butterflies. We conclude that larval food-stress does not elicit compensatory feeding behavior such as a stronger preference for amino acids or other essential nutrients in B. anynana. Instead, the stress imposed by a period of starvation yielded negative effects. Copyright © 2012 Elsevier Ltd. All rights reserved.

  4. Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse.

    Science.gov (United States)

    Thabet, Olfa Ben Dhia; Fardeau, Marie-Laure; Joulian, Catherine; Thomas, Pierre; Hamdi, Moktar; Garcia, Jean-Louis; Ollivier, Bernard

    2004-06-01

    A new sporulated fermentative bacterium designated strain E1(T) (T=type strain), was isolated from an anaerobic mud of an olive mill wastewater basin contaminated by phosphogypse produced by a Tunisian factory. Strain E1(T) was a motile Gram-positive slightly curved rod with spherical terminal spore swelling the cell. It grew between 18 degrees C and 43 degrees C with an optimum at 37 degrees C and pH 7.8 (range 5.5-8.7), without NaCl (range 0-3%). Strain E1(T) was a chemoorganotrophic anaerobic bacterium fermenting only proteins and amino acids. Yeast extract was required for growth. Elemental sulfur was used as terminal electron acceptor. The G+C content of the DNA was 32.6 mol%. The closest phylogenetical relatives of strain E1(T) were Clostridium thiosulfatireducens and C. subterminale (97.3% similarity for partial rRNA gene sequences). DNA-DNA hybridization values between strain E1(T) and both species were 17% and 20.8%, respectively. On the basis of differences in genotypic and phenotypic characteristics, strain E1(T) (DSM 15206(T), CIP 107666(T)) is proposed as the type strain of a new species, C. tunisiense sp. nov. GenBank accession number for the 16S rRNA gene sequence of strain E1(T) is AY187622.

  5. Physiological requirements for growth and competitiveness of Dekkera bruxellensis under oxygen-limited or anaerobic conditions.

    Science.gov (United States)

    Blomqvist, Johanna; Nogué, Violeta Sànchez; Gorwa-Grauslund, Marie; Passoth, Volkmar

    2012-07-01

    The effect of glucose and oxygen limitation on the growth and fermentation performances of Dekkera bruxellensis was investigated in order to understand which factors favour its propagation in ethanol or wine plants. Although D. bruxellensis has been described as a facultative anaerobe, no growth was observed in mineral medium under complete anaerobiosis while growth was retarded under severe oxygen limitation. In a continuous culture with no gas inflow, glucose was not completely consumed, most probably due to oxygen limitation. When an air/nitrogen mixture (O(2)-content ca. 5%) was sparged to the culture, growth became glucose-limited. In co-cultivations with Saccharomyces cerevisiae, ethanol yields/g consumed sugar were not affected by the co-cultures as compared to the pure cultures. However, different population responses were observed in both systems. In oxygen-limited cultivation, glucose was depleted within 24 h after challenging with S. cerevisiae and both yeast populations were maintained at a stable level. In contrast, the S. cerevisiae population constantly decreased to about 1% of its initial cell number in the sparged glucose-limited fermentation, whereas the D. bruxellensis population remained constant. To identify the requirements of D. bruxellensis for anaerobic growth, the yeast was cultivated in several nitrogen sources and with the addition of amino acids. Yeast extract and most of the supplied amino acids supported anaerobic growth, which points towards a higher nutrient demand for D. bruxellensis compared to S. cerevisiae in anaerobic conditions. Copyright © 2012 John Wiley & Sons, Ltd.

  6. Coordinated surface activities in Variovorax paradoxus EPS

    Directory of Open Access Journals (Sweden)

    Gregory Glenn A

    2009-06-01

    Full Text Available Abstract Background Variovorax paradoxus is an aerobic soil bacterium frequently associated with important biodegradative processes in nature. Our group has cultivated a mucoid strain of Variovorax paradoxus for study as a model of bacterial development and response to environmental conditions. Colonies of this organism vary widely in appearance depending on agar plate type. Results Surface motility was observed on minimal defined agar plates with 0.5% agarose, similar in nature to swarming motility identified in Pseudomonas aeruginosa PAO1. We examined this motility under several culture conditions, including inhibition of flagellar motility using Congo Red. We demonstrated that the presence of a wetting agent, mineral, and nutrient content of the media altered the swarming phenotype. We also demonstrated that the wetting agent reduces the surface tension of the agar. We were able to directly observe the presence of the wetting agent in the presence and absence of Congo Red, and found that incubation in a humidified chamber inhibited the production of wetting agent, and also slowed the progression of the swarming colony. We observed that swarming was related to both carbon and nitrogen sources, as well as mineral salts base. The phosphate concentration of the mineral base was critical for growth and swarming on glucose, but not succinate. Swarming on other carbon sources was generally only observed using M9 salts mineral base. Rapid swarming was observed on malic acid, d-sorbitol, casamino acids, and succinate. Swarming at a lower but still detectable rate was observed on glucose and sucrose, with weak swarming on maltose. Nitrogen source tests using succinate as carbon source demonstrated two distinct forms of swarming, with very different macroscopic swarm characteristics. Rapid swarming was observed when ammonium ion was provided as nitrogen source, as well as when histidine, tryptophan, or glycine was provided. Slower swarming was observed

  7. Caloramator quimbayensis sp. nov., an anaerobic, moderately thermophilic bacterium isolated from a terrestrial hot spring.

    Science.gov (United States)

    Rubiano-Labrador, Carolina; Baena, Sandra; Díaz-Cárdenas, Carolina; Patel, Bharat K C

    2013-04-01

    An anaerobic, moderately thermophilic, terminal-spore-forming bacterium, designated strain USBA A(T), was isolated from a terrestrial hot spring located at an altitude of 2683 m in the Andean region of Colombia (04° 50' 14.0″ N 75° 32' 53.4″ W). Cells of strain USBA A(T) were Gram-stain-positive, straight to slightly curved rods (0.9×2.5 µm), that were arranged singly or in pairs, and were motile by means of flagella. Growth occurred at 37-55 °C and pH 6.0-8.0, with a doubling time of 2 h under the optimal conditions (50 °C and pH 7.0). Glucose fermentation in strain USBA A(T) required yeast extract or peptone (each at 0.2 %, w/v). The novel strain fermented sugars, amino acids, Casamino acids, propanol, propionate, starch and dextrin, but no growth was observed on galactose, lactose, xylose, histidine, serine, threonine, benzoate, butyrate, lactate, pyruvate, succinate, methanol, ethanol, glycerol, casein, gelatin or xylan. The end products of glucose fermentation were formate, acetate, ethanol and lactate. Strain USBA A(T) did not grow autotrophically (with CO2 as carbon source and H2 as electron donor) and did not reduce thiosulfate, sulfate, elemental sulfur, sulfite, vanadium (V) or Fe (III) citrate. Growth of strain USBA A(T) was inhibited by ampicillin, chloramphenicol, kanamycin, penicillin and streptomycin (each at 10 µg ml(-1)). The predominant fatty acids were iso-C15 : 0, C16 : 0 and iso-C17 : 0 and the genomic DNA G+C content was 32.6 mol%. 16S rRNA gene sequence analysis indicated that strain USBA A(T) belonged in the phylum Firmicutes and that its closest relative was Caloramator viterbiensis JW/MS-VS5(T) (95.0 % sequence similarity). A DNA-DNA relatedness value of only 30 % was recorded in hybridization experiments between strain USBA A(T) and Caloramator viterbiensis DSM 13723(T). Based on the phenotypic, chemotaxonomic and phylogenetic evidence and the results of the DNA-DNA hybridization experiments, strain USBA A

  8. A gene between polA and glnA retards growth of Escherichia coli when present in multiple copies: physiological effects of the gene for spot 42 RNA.

    Science.gov (United States)

    Rice, P W; Dahlberg, J E

    1982-12-01

    We have isolated the single gene for spot 42 RNA of Escherichia coli on a 20-kilobase DNA fragment. Physical characterization of this cloned DNA fragment showed that it is homologous to a region at 86 min on the genetic map and extends from the 23S to 5S rRNA coding region of rrnA to the coding region of glnA, the gene for glutamine synthetase. Other genes included on this cloned DNA fragment are polA, ntrC (glnG), and ntrB (glnL). E coli cells transformed with a multicopy plasmid clone of the gene for spot 42 RNA had about a 10-fold increase in the amount of spot 42 RNA they contained. The amount of 6S RNA in these cells was increased about twofold, although the gene for 6S RNA was not located on this plasmid or on the larger 20-kilobase fragment. Presence of this multicopy plasmid also affected the growth of cells. The generation time was increased under a variety of growth conditions, especially when cells were grown in medium with succinate as the carbon source. In addition, some strains of E. coli which have multicopy plasmids carrying the gene for spot 42 RNA were unable to respond normally to a shift into richer medium: upon upshift from minimal glucose to LB broth or minimal glucose plus 1% Casamino Acids, there was a 3- to 4-h lag before the culture adapted to the new medium. More than 90% of the cells in such cultures stopped dividing, although they remained viable. The plating efficiency of minimal-glucose-grown cells was 100-fold less on rich media than on minimal glucose medium. One revertant was isolated which regained the phenotype of pBR322-transformed cells. Analysis of this strain showed that the plasmid it contained had an insertion of an IS1 element into the 5' end of the coding region for the gene for spot 42 RNA.

  9. Coordinated surface activities in Variovorax paradoxus EPS.

    Science.gov (United States)

    Jamieson, W David; Pehl, Michael J; Gregory, Glenn A; Orwin, Paul M

    2009-06-12

    Variovorax paradoxus is an aerobic soil bacterium frequently associated with important biodegradative processes in nature. Our group has cultivated a mucoid strain of Variovorax paradoxus for study as a model of bacterial development and response to environmental conditions. Colonies of this organism vary widely in appearance depending on agar plate type. Surface motility was observed on minimal defined agar plates with 0.5% agarose, similar in nature to swarming motility identified in Pseudomonas aeruginosa PAO1. We examined this motility under several culture conditions, including inhibition of flagellar motility using Congo Red. We demonstrated that the presence of a wetting agent, mineral, and nutrient content of the media altered the swarming phenotype. We also demonstrated that the wetting agent reduces the surface tension of the agar. We were able to directly observe the presence of the wetting agent in the presence and absence of Congo Red, and found that incubation in a humidified chamber inhibited the production of wetting agent, and also slowed the progression of the swarming colony. We observed that swarming was related to both carbon and nitrogen sources, as well as mineral salts base. The phosphate concentration of the mineral base was critical for growth and swarming on glucose, but not succinate. Swarming on other carbon sources was generally only observed using M9 salts mineral base. Rapid swarming was observed on malic acid, d-sorbitol, casamino acids, and succinate. Swarming at a lower but still detectable rate was observed on glucose and sucrose, with weak swarming on maltose. Nitrogen source tests using succinate as carbon source demonstrated two distinct forms of swarming, with very different macroscopic swarm characteristics. Rapid swarming was observed when ammonium ion was provided as nitrogen source, as well as when histidine, tryptophan, or glycine was provided. Slower swarming was observed with methionine, arginine, or tyrosine. Large

  10. Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae.

    Science.gov (United States)

    Rehman, Latifur; Su, Xiaofeng; Guo, Huiming; Qi, Xiliang; Cheng, Hongmei

    2016-07-26

    Large efforts have focused on screening for genes involved in the virulence and pathogenicity of Verticillium dahliae, a destructive fungal pathogen of numerous plant species that is difficult to control once the plant is infected. Although Agrobacterium tumefaciens-mediated transformation (ATMT) has been widely used for gene screening, a quick and easy method has been needed to facilitate transformation. High-quality protoplasts, with excellent regeneration efficiency (65 %) in TB3 broth (yeast extract 30 g, casamino acids 30 g and 200g sucrose in 1L H20), were generated using driselase (Sigma D-9515) and transformed with the GFP plasmid or linear GFP cassette using PEG or electroporation. PEG-mediated transformation yielded 600 transformants per microgram DNA for the linear GFP cassette and 250 for the GFP plasmid; electroporation resulted in 29 transformants per microgram DNA for the linear GFP cassette and 24 for the GFP plasmid. To determine whether short interfering RNAs (siRNAs) can be delivered to the protoplasts and used for silencing genes, we targeted the GFP gene of Vd-GFP (V. dahliae GFP strain obtained in this study) by delivering one of four different siRNAs-19-nt duplex with 2-nt 3' overhangs (siRNA-gfp1, siRNA-gfp2, siRNA-gfp3 and siRNA-gfp4)-into the Vd-GFP protoplasts using PEG-mediated transformation. Up to 100 % silencing of GFP was obtained with siRNA-gfp4; the other siRNAs were less effective (up to 10 % silencing). Verticillium transcription activator of adhesion (Vta2) gene of V. dahliae was also silenced with four siRNAs (siRNA-vta1, siRNA-vta2, siRNA-vta3 and siRNA-vta4) independently and together using the same approach; siRNA-vta1 had the highest silencing efficiency as assessed by colony diameter and quantitative real time PCR (qRT-PCR) analysis. Our quick, easy transformation method can be used to investigate the function of genes involved in growth, virulence and pathogenicity of V. dahliae.

  11. Lactobacillus gasseri requires peptides, not proteins or free amino acids, for growth in milk.

    Science.gov (United States)

    Arakawa, K; Matsunaga, K; Takihiro, S; Moritoki, A; Ryuto, S; Kawai, Y; Masuda, T; Miyamoto, T

    2015-03-01

    Lactobacillus gasseri is a widespread commensal lactic acid bacterium inhabiting human mucosal niches and has many beneficial effects as a probiotic. However, L. gasseri is difficult to grow in milk, which hurts usability for the food industry. It had been previously reported that supplementation with yeast extract or proteose peptone, including peptides, enables L. gasseri to grow well in milk. In this study, our objective was to confirm peptide requirement of L. gasseri and evaluate efficacy of peptide release by enzymatic proteolysis on growth of L. gassei in milk. Three strains of L. gasseri did not grow well in modified DeMan, Rogosa, Sharpe broth without any nitrogen sources (MRS-N), but addition of a casein-derived peptide mixture, tryptone, promoted growth. In contrast, little effect was observed after adding casein or a casein-derived amino acid mixture, casamino acids. These results indicate that L. gasseri requires peptides, not proteins or free amino acids, among milk-derived nitrogen sources for growth. Lactobacillus gasseri JCM 1131T hardly had growth capacity in 6 kinds of milk-based media: bovine milk, human milk, skim milk, cheese whey, modified MRS-N (MRSL-N) supplemented with acid whey, and MRSL-N supplemented with casein. Moreover, treatment with digestive proteases, particularly pepsin, to release peptides made it grow well in each milk-based medium. The pepsin treatment was the most effective for growth of strain JCM 1131T in skim milk among the tested food-grade proteases such as trypsin, α-chymotrypsin, calf rennet, ficin, bromelain, and papain. As well as strain JCM 1131T, pepsinolysis of milk improved growth of other L. gasseri strains and some strains of enteric lactobacilli such as Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus johnsonii, and Lactobacillus reuteri. These results suggest that some relatives of L. gasseri also use peptides as desirable nitrogen sources, and that milk may be a good supplier of nutritious

  12. Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio.

    Science.gov (United States)

    Zavarzina, D G; Zhilina, T N; Tourova, T P; Kuznetsov, B B; Kostrikina, N A; Bonch-Osmolovskaya, E A

    2000-05-01

    A moderately thermophilic, organotrophic bacterium with vibrioid cells was isolated from a sample of a cyanobacterial mat from caldera Uzon, Kamchatka, Russia, and designated strain Z-9701T. Cells of strain Z-9701T were curved, Gram-negative rods, 0.5-0.7 x 2.5-5.0 microm in size, with tapering ends and with fast, wavy movement by means of lateral flagella located on the concave side of the cell. Colonies were small, white, irregular or round, 0.2 mm in diameter, and with even edges. Strain Z-9701T was an obligate anaerobe with a temperature optimum at 60-65 degrees C and a pH optimum at 7.3. It fermented glucose, fructose, mannose, N-acetyl-D-glucosamine, adonite, arginine, serine, peptone, yeast extract and Casamino acids. The fermentation products formed during growth on glucose were acetate, lactate, H2, CO2 and ethanol. Strain Z-9701T reduced elemental sulfur to H2S during organotrophic growth with glucose or peptides as energy and carbon sources. In the presence of S0, strain Z-9701T was capable of lithotrophic growth with molecular hydrogen as energy substrate and 0.1 g yeast extract l(-1) as carbon source. Sulfate, thiosulfate, nitrate, Fe(III) and sulfite were not reduced and did not stimulate growth. The G+C content of strain Z-9701T DNA was 54.6 mol%. The results of 16S rDNA sequence analyses revealed that strain Z-9701T belongs to the cluster within the Clostridium group formed by Thermanaerovibrio acidaminovorans, Dethiosulfovibrio peptidovorans, Anaerobaculum thermoterrenum and Aminobacterium colombiense, but the level of sequence similarity with the members of this cluster was not very high (87.6-92.2%). Among these organisms, Thermanaerovibrio acidaminovorans is phenotypically close to strain Z-9701T. However, the two organisms showed a relatively low level of similarity of their 16S rRNA sequences (92.2%) and of DNA-DNA hybridization (15 +/- 1%). Nevertheless, on the basis of the similar morphology and physiology of the new isolate and

  13. Structural Consequences of Calmodulin EF Hand Mutations.

    Science.gov (United States)

    Piazza, Michael; Taiakina, Valentina; Dieckmann, Thorsten; Guillemette, J Guy

    2017-02-21

    Calmodulin (CaM) is a cytosolic Ca(2+)-binding protein that serves as a control element for many enzymes. It consists of two globular domains, each containing two EF hand pairs capable of binding Ca(2+), joined by a flexible central linker region. CaM is able to bind and activate its target proteins in the Ca(2+)-replete and Ca(2+)-deplete forms. To study the Ca(2+)-dependent/independent properties of binding and activation of target proteins by CaM, CaM constructs with Ca(2+)-binding disrupting mutations of Asp to Ala at position one of each EF hand have been used. These CaM mutant proteins are deficient in binding Ca(2+) in either the N-lobe EF hands (CaM12), C-lobe EF hands (CaM34), or all four EF hands (CaM1234). To investigate potential structural changes these mutations may cause, we performed detailed NMR studies of CaM12, CaM34, and CaM1234 including determining the solution structure of CaM1234. We then investigated if these CaM mutants affected the interaction of CaM with a target protein known to interact with apoCaM by determining the solution structure of CaM34 bound to the iNOS CaM binding domain peptide. The structures provide direct structural evidence of changes that are present in these Ca(2+)-deficient CaM mutants and show these mutations increase the hydrophobic exposed surface and decrease the electronegative surface potential throughout each lobe of CaM. These Ca(2+)-deficient CaM mutants may not be a true representation of apoCaM and may not allow for native-like interactions of apoCaM with its target proteins.

  14. Improving corn silage quality in the top layer of farm bunker silos through the use of a next-generation barrier film with high impermeability to oxygen.

    Science.gov (United States)

    Borreani, G; Tabacco, E

    2014-01-01

    This study examined the effect on the fermentation, chemical, and microbiological quality of corn silage covered with a new-generation high oxygen barrier film (HOB) made with a special grade of ethylene-vinyl alcohol (EVOH) compared with a standard polyethylene film (PE). Two bunkers (farms 1 and 2) were divided into 2 parts lengthwise so that half of the silo would be covered with PE film and the other with HOB film. Plastic net bags with fresh chopped corn were buried in the upper layer (close to and far from the wall) and in the central part of the bunkers. During spring-summer consumption, the bags were unloaded, weighed, and subsampled to analyze the dry matter (DM) content, neutral detergent fiber and starch contents, pH, lactic and monocarboxylic acids, yeast and mold counts, aerobic and anaerobic spore-former counts, and aerobic stability. We also determined the economic benefit of applying the novel covering. The top layer of silage conserved under the HOB film had a higher lactic acid content and lower pH; lower counts of yeasts, molds, and aerobic and anaerobic spore-formers; higher aerobic stability; and lower DM losses than the silage conserved under the PE film. The use of the HOB film prevented almost all of the silage in the upper layer from spoiling; only 2 out of 32 samples had a mold count >6log10 cfu/g. This led to a net economic gain when the HOB film was used on both farms due to the increased DM recovery and reduced labor time required to clean the upper layer, even though the HOB film cost about 2.3 times more than the PE film. Furthermore, use of the HOB film, which ensures a longer shelf life of silage during consumption, reduced the detrimental effect of yeasts, molds, and aerobic and anaerobic spore-formers on the nutritional and microbiological quality of the unloaded silage.

  15. Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

    Science.gov (United States)

    Zhang, Jun-Hui; Jiang, Yu-Yan; Lin, Ying; Sun, Yu-Fei; Zheng, Sui-Ping; Han, Shuang-Yan

    2013-01-01

    To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257Leu were obtained and their hydrolytic and esterification activities were assayed. Using Discovery Studio 3.1 to build models and calculate the binding energy between lipase and substrates, compared to wild-type, the mutant Asp256Ile/His257Leu was found to have significantly lower energy when oleic acid (3.97 KJ/mol decrease) and tripalmitin (7.55 KJ/mol decrease) were substrates. This result was in accordance with the esterification activity of Asp256Ile/His257Leu (2.37-fold of wild-type). The four mutants were also evaluated for the production of HMFS in organic solvent and in a solvent-free system. Asp256Ile/His257Leu had an oleic acid incorporation of 28.27% for catalyzing tripalmitin and oleic acid, and 53.18% for the reaction of palm oil with oleic acid. The efficiency of Asp256Ile/His257Leu was 1.82-fold and 1.65-fold that of the wild-type enzyme for the two reactions. The oleic acid incorporation of Asp256Ile/His257Leu was similar to commercial Lipozyme RM IM for palm oil acidolysis with oleic acid. Yeast surface-displayed RML mutant Asp256Ile/His257Leu is a potential, economically feasible catalyst for the production of structured lipids.

  16. Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

    Directory of Open Access Journals (Sweden)

    Jun-Hui Zhang

    Full Text Available To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML in the production of human milk fat substitute (HMFS, we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257Leu were obtained and their hydrolytic and esterification activities were assayed. Using Discovery Studio 3.1 to build models and calculate the binding energy between lipase and substrates, compared to wild-type, the mutant Asp256Ile/His257Leu was found to have significantly lower energy when oleic acid (3.97 KJ/mol decrease and tripalmitin (7.55 KJ/mol decrease were substrates. This result was in accordance with the esterification activity of Asp256Ile/His257Leu (2.37-fold of wild-type. The four mutants were also evaluated for the production of HMFS in organic solvent and in a solvent-free system. Asp256Ile/His257Leu had an oleic acid incorporation of 28.27% for catalyzing tripalmitin and oleic acid, and 53.18% for the reaction of palm oil with oleic acid. The efficiency of Asp256Ile/His257Leu was 1.82-fold and 1.65-fold that of the wild-type enzyme for the two reactions. The oleic acid incorporation of Asp256Ile/His257Leu was similar to commercial Lipozyme RM IM for palm oil acidolysis with oleic acid. Yeast surface-displayed RML mutant Asp256Ile/His257Leu is a potential, economically feasible catalyst for the production of structured lipids.

  17. An Implementation of the REpresentation and MAintenance of Process Knowledge(REMAP) Model in the Knowledge-Based Software Assistant Concept Demonstration System

    Science.gov (United States)

    1993-09-01

    D.M. Elefante , p. 8, 1988. 8. Debellis, M., &#34The Concept Demonstration Rapid Prototype System", PROCEEDINGS OF THE FIFTH CONFERENCE ON KBSA, p. 222...1987. 11. Elefante , loc. cit., p. 8. 12. White, op. cit., p. 3. 13. White, op. cit., p. 4. 14. Goldberg, A., et al., "KBSA Performance Estimation...Assistant Program Specification," Documentation, RADC Contract F30602-86-C-0026. 71 15. White, op.cit., p. 5. 16. White, op. cit., pp. 3-4. 17. Elefante , op

  18. Event display of a 4-muon candidate in the ATLAS detector

    CERN Multimedia

    ATLAS, Collaboration

    2012-01-01

    Event display of a 4mu candidate. EventNumber: 71902630 RunNumber: 204769 m_4l=125.1 GeV. m_12=86.3 GeV, m_34=31.6 GeV. mu_1: pt=36.1 GeV, eta=1.29, phi=1.33. mu_2: pt=47.5 GeV, eta=0.69, phi=-1.65. mu_3: pt=26.4 GeV, eta=0.47, phi=-2.51. mu_4: pt=71.7 GeV, eta=1.85, phi=1.65.

  19. Composite Failure Analysis Handbook. Volume 2: Technical Handbook. Part 1 - Procedures and Techniques

    Science.gov (United States)

    1992-02-01

    of a wrong :Pa~tArial much as a 250,~F curing prepirte in pl~acc of a :350’F curing 6-201 I . . . . i • I ’. t .H - CH SU Epoxude funcional .I ..i . I...and Physics, 65th edition, R.C. Weast (ed.), CRC Press Inc., Boca Raton, Florida, 1985, p. E-130. 6. Ratner, B.D., McElroy, B.J., in Spectroscopy in...the Biomedical Sciences, R.M. Gendreau (ed.), CRC Press Inc., Boca Raton, Florida, 1986, pp. 107-140. @ 7. Daniel, I.M., &#34The Behavior of Uniaxial

  20. Dicty_cDB: Contig-U09742-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available umbec), complet... 34 0.38 2 ( DQ441422 ) Variola virus strain Bangladesh 1974 (Solaiman), ... 34 0.38 2 ( D...Q441421 ) Variola virus strain Bangladesh 1974 (Shahzaman),... 34 0.38 2 ( DQ441420 ) Variola virus strain Bangladesh... 2 ( DQ441427 ) Variola virus strain India 1953 (Kali-Muthu-M50 M... 34 0.38 2 ( L22579 ) Variola major virus (strain Bangladesh...South Africa 1965 (102 Natal... 34 0.38 2 ( DQ437581 ) Variola virus strain Bangladesh 1975 v75-550 Banu...

  1. Tactical Nuclear Weapons in the Post Cold War Era: Implications for the Operational Commander

    Science.gov (United States)

    1993-01-01

    the Nerval War Coll~ In. ~ a]satisfaction of the requi 6rlent" Cf A Dartrent ol Op ~at~a. T*crt ~f- Pu- a Pam ef1ec ~ perscrlal views a a-e not...Longman Publishing Group, 1991, p.67. 6. Ibid., p.67. 7. Ibid., pp. 67-69. 8. Gerald M. Steinberg, "Towards Real Arms Control In the Middle East...Report for Congress. June 5, 1992. Steinberg, Gerald M., &#34Towards Real Arms Control In the Middle East". Issues in Science and Technology. Vol. IV, Summer

  2. Proceedings of the 1983 Spring Meeting of the Packaging, Handling and Transportability Division of the American Defense Preparedness Association Held at Port Hueneme, California on April 26-28, 1983

    Science.gov (United States)

    1983-01-01

    type A packaging , 173.398; shipments by Air, 173.6; * molded or thermoformed polyethlene packaging , 178.35a; packging for blasting agents, 173.1 14a...ýtSE V PROCEEDINGS 1983 SPRING 0 C IAl’oMEETING 4 Packaging , Handling and Transportablit~y Division ½C3C ~American Def ense ~T1CPreparedness...Founded 1919 FORT.,-D11M&#34 The 1983 Tpring Meeting of the Packaging , Handling and Transportability Divisicn of the American Defense Preparedness

  3. An Annotated Bibliography on the Biological Effects of Constructing Channels, Jetties, and other Coastal Structures.

    Science.gov (United States)

    1983-01-01

    18. BRUER, J.P., "An Ecological Survey of the Lower Laguna Madre of Texas , 1953- 1959," Publications of the Institute of Marine Science, Vol. 8...the lower Laguna Madre of Texas , a shallow, hyper- saline marine bay extending 122 kilometers north of the Rio Grande delta. In summer, waters of the...DINEEN, C., and DARNELL, R.M, &#34The Effects of Shell Dredging on the Food Habits of Fishes in San Antonio Bay, Texas ," Environmental Impact Assess- ment of

  4. Choppers Grounded: The Supply-Demand Problem

    Science.gov (United States)

    1988-01-01

    Mtulleni focuses in this essaxy on the availabl~Iitv of it si iigle. h ioh - tehnooy weapon system --the lii 1-ti)i i t’leI p ter. In an opening sc;enar io...priorities iind~ spend more for defense. Mobilization of US forces, in all lWelHOoM &#34dl ý!eMmerate a global miobilization of thme Free k\\orld which. in) turn... mobilized nation to one, with extreniely limited MO- hilIizat ion potential o(:curedl over it 30-year period. As t he overwhel muinxgly major holder of

  5. Dredged-Material Disposal System Capacity Expansion.

    Science.gov (United States)

    1986-04-01

    plan is the product of the acquisition cost and the binary variable. O’Laoghaire and Himmelblau (1972) propose such an IP formulation, and a similar...Methods: A Survey," Op- erations Research, Vol. 14, 1966, pp. 699-719. Lesso, W. G., Himmelblau , D. M., Jensen, P. A., and Shanmugham, C. V., "Ca...34 Water Resources Research, Vol. 15, No. 4, Aug., 1979, pp. 750-756. O’Laoghaire, D. T., and Himmelblau , D. M., &#34Optimal Capital Investment in the

  6. Imaging Molecular Signatures of Breast Cancer With X-ray Activated Nano-Phosphors

    Science.gov (United States)

    2011-09-01

    shot-noise limited detection" was assumed to be too low to detect. To calculate the molar concentration, we assumed a spherical 10 nm diameter...Kandarakis et al. !a" Minimum weight concentration !in mg/ml" vs dose !Gy". !b" Minimum molar concentration !in M&#34 vs dose !Gy". a b c d FIG. 5. Agar imaging...Contrast agents: X-ray contrast agents and molecular imaging—A contradiction ?,” Handb Exp Pharmacol 185, 167–175 񓟸#. 3R. Weissleder and M. J. Pittet

  7. Mikhail Gorbachev’s ’New Thinking’ Implications for Western Security

    Science.gov (United States)

    1991-06-01

    for thf Advancement of Slavic Studies in Boston, MA, 5-7 November 1987. Citation is from p. 3. ’nSee David S. Yost, Soviet Ballistic Missile Defense...of the American Association for the Advancement of Slavic Studies , Boston, MA, 5-7 November 1987. "The New Quality of Soviet Defense." international...Association for the Advancement of Slavic Studies , Boston, MA, 5-7 November, 1987. McConnell, James M. &#34Reasonable Suffidency" in Soviet Conventional Arms

  8. The Malvinas War from the Argentinian Viewpoint

    Science.gov (United States)

    1988-05-01

    Guerra Aerea. Buenos Aires, October 1982. - Malvinas . Testiaco de Batallas. J. Romero and S. Mate --Ed. Federico Domenech. Valencia, 1984. -The Falklands...DTI( -FI)AC OTIC FORm 70A DOCUMENT PROCESSING SHEET 5.. 5’A1 BE USE MAR 86 I’ M &#34THE MALVINAS WAR FROM THE ARGENTINIAN VIEWPOINTŔ LT COL RICARDO...UNIVERSITY "THE MALVINAS WAR FROM THE ARGENTINIAN VIEWPOINT" by Ricardo Albert Puche .Lt. Col, SPANISH AIR FORCE A RESEARCH REPORT SUBMITTED TO THE FACULTY

  9. An Architecturally-Based Theory of Human Sentence Comprehension

    Science.gov (United States)

    1993-12-18

    22 hcci -I and I’ I)ave occupv firs; and second comphlement positions of Ievel spec-lP: Lt, JohnT , IA I’lftl R[m- (&#34F s I: Comnp V’ . ’ • ctfll .2 1...within thi s rUCtRI The richness of the Interactionis arnoiig the gramimatical constraints is the engine that builds up aChu ii king.) fie areSet Of C...thesis, The University of Nile’i an. Department of Electricral Engineering an d Computer Science. Forthcoming. John, B. FI. (; 988). Contributioias to

  10. Laser-Induced Thermal Damage of Skin

    Science.gov (United States)

    1977-12-01

    As with the eye model, ther- mal damage is predicted using Henriques damage integral (11). This criterion involves integrating temperature- dependent...epide•: mal layers are much larger than the coefficients for the entire skin presented in Figure 5. This observation suggests there should be twd...EXPOI"’ES INVOLVJINS MUMtPLE PULSES OTOODYCIZO 010OTO OJO SI tNGLE PUL.$LSE 19(M.&#34ft 7.13NP#R5 xxsLlimft p DO 3 ?V 36 Ep(MgiSNPRCL) *~To so 36

  11. Parallel Interpolation Search.

    Science.gov (United States)

    1982-03-01

    table keys to be read in the next step. (Note that as in the previous literature on search algorithms, for example [Yao and Yao, 76], (Perl and Itai , 78...Knuth, 73]; subsequently it was independently shown to be loglog(n) + 0(l) by (Yao and Yao, 761, [Perl and Itai , 78] and [Gonnet, Rogers, and George, 80...and Reingold, E.M., &#34Understanding and Complexity of Interpo- lation Search," Infrm. Proc. Letters, 6 (1977), 219-222. Perl, Y., Itai , A., Avni, H

  12. Pyrophaeophorbide-a as a tracer of suspended particulate organic matter from the NE Pacific continental margin

    OpenAIRE

    Thomas S. Bianchi; Bauer, James E.; Druffel, Ellen R. M.; Lambert, Corey D

    1998-01-01

    Pyrophaeophorbide-a, a degradation product of chlorophyll-a, is predominantly formed by grazing processes in sediments as well as in the water column. Water column profiles of pyrophaeophorbide-a/suspended particulate organic carbon (SPOC) concentrations, at an abyssal site in the northeast (NE) Pacific (Sta M, 34°50′N, 123°00′W; 4100 m water depth), show low concentrations (0.01–0.1 ng/μg SPOC) at surface and mesopelagic depths, and increasing concentrations with closer proximity to the sea ...

  13. Benign Breast Disease: Toward Molecular Prediction of Breast Cancer Risk

    Science.gov (United States)

    2009-06-01

    papilloma virus E7 (HPV-E7) caused a robust upregulation of COX-2 expression and sensitizes cells to COX-2 induction by exogenous inducers such as TGF... teri ,tic Non-rrolil~.rutive uiM>:&#34l>c Ow mil 6,244 (117.4%) A’t$> :..l biOfisY ឝ 3,300 (76.5%) 45- 55 l.ti.’i7 (1\\2.7%) >55 1.287 (6’\\.J

  14. Initiative in Concurrent Engineering (DICE). Phase 1.

    Science.gov (United States)

    1990-02-09

    Manufacturing (MF), and Project Lead (PL). &bI= t" ’"M(&#34 ""... Ann • DnwA EID Iie ’) ’vaDs4UgIa pc abe .2gII . 1". ,e .. **5 E 1.1 mnN.2. I...4. Reforming Fixture (DARPA DICE 7006-C) 5. EDM Preparation Fixture Shroud Tip (DARPA DICE 0701-T2) * 6. Guillotine Inspection Gauge 7. Copper ECM...indicate that we must continue to build on the so called " Taguchi methods" and other techniques in an effort to produce more robust products; 2. There

  15. Novel Laser-Based Technique for Measurements of Primary Atomization Characteristics of Liquid Jets

    Science.gov (United States)

    2012-08-22

    continuous liquid jet has been suggested (6). Corona discharge is unlikely to cause current through the gas between the liquid nozzle and the probe...H., Shimizu M., and Arai M., &#34The breakup of high speed jet in a high pressure gaseous atmosphere ," ICLASS-82, 1982. 10. Cai, W.Y., Powell, C.F...speed jet in a high pressure gaseous atmosphere ". ICLASS-82, 1982. 10. Cai, W.Y., Powell, C.F., Yue, Y., Narayanan, S., Wang, J., Tate, M.W., Renzi

  16. Selected Bibliography on Fiber-Reinforced Cement and Concrete. Supplement Number 4.

    Science.gov (United States)

    1982-08-01

    Building Industry," L’Industria Italiana del Cemento , Vol 50, No. 12, Dec 1980, pp 1135-1144. 19. Bartos, P., "Pullout Failure of Fibres Embedded in Cement...Vol 43, No. 11, Nov 1977, pp 561-564. 21. Bassan, M., &#34Model of Behavior of Fiber-Reinforced Concretes Under Impact Stresses," il Cemento , Vol 74, No...Pastes," il Cemento , Vol 75, No. 3, Jul-Sep 1978, pp 277-284. 210. Mills, R. H., "Age-Embrittlement of Glass-Reinforced Concrete Containing Blastfurance

  17. Effect of Gas/Water Ratio on the Performance of Combined Cylindrical Anoxic/Aerobic Moving Bed Biofilm Reactors for Biological Nutrients Removal from Domestic Wastewater by Fully Nitrification-Denitrification Processes

    Directory of Open Access Journals (Sweden)

    Husham T. Ibrahim

    2014-04-01

    Full Text Available In this research the continuously up-flow pilot scale Moving Bed Biofilm Reactor (MBBR which was consists of combined cylindrical anoxic/aerobic MBBR in nested form with anoxic/aerobic volume ratio equal to 0.16 under fully nitrification-denitrification process were used to treated 4 m34+-N, TN and TP, respectively, while the average Dissolved Oxygen concentration (DO in aerobic and anoxic MBBRs were 4.49 and 0.16 mg/L, respectively.

  18. The Impact of Petroleum, Synthetic and Cryogenic Fuels on Civil Aviation.

    Science.gov (United States)

    1982-06-01

    1981. 1980, Washington. 3-9. Keller, George M., &#34Economic Recovery Would Spur 3-2. Van Vuren, Raymond , "The ATC Phoenix," FAA World, Crude Demand," p... Bayer of the U.S. Geological Survey has joked (7 16) that < 0.3 3.00 between five and ten thousand wells are producing commercial 0.2 - natural gas from...January 25, 1982. Congress, January 1981. 7-3. U.S. Congress, The Natural Gas Policy Act of 1978. 7-16. Bayer , K.C., (paper not available), U.S

  19. Tax-Exempt Bonds: Retirement Center Bonds Were Risky and Benefited Moderate-Income Elderly.

    Science.gov (United States)

    1991-03-01

    accordance with generally accepted government auditing standards. We obtained 0 0 informal comments from AAhA and the Public Securities Association...and M&#34y PFe by Tye of Unit Engannc foe Memonly fi "Type of nt Low High Low High Studio/efficiency $30,416 $37,060 $884 S1,007 One-bedroom 47,825...by-case basis when an organization files for tax-exempt status or during subsequent audits . We did not eval- uate is enforcement of this ruling. Our

  20. Global Climate Change (GCC) Issues and Their Impacts on the US Army Corps of Engineers

    Science.gov (United States)

    1991-11-01

    year-to-year or decade-to-decade [3-8]. Solow and Broadus [3-9] challenged Hansen’s view that the greenhouse effect was the reason for the warming in...A.. "Global Temperature Hits Record Again." Science 251. (18 January 1991): 274. [3-9] Solow , Andrew R. and Bradus, James M.. &#34On the Detection of...4] Albritton, Daniel L., "Stratospheric Ozone Depletion: Global Processes," National Academy Press (March 1988): pp. 10-18. [9-5] Battiata, Mary

  1. Energy Functions for Early Vision and Analog Networks.

    Science.gov (United States)

    1987-11-01

    Feynman 1963). * For example in the way the noise thresholds are determined locally for the Canny Iedge detector. 0° 36 tion "captures" the spots and...integrable if, and only if, dI = 0 where d is the exterior derivative operator (Misner, Thorne, Wheeler , 1977). Define Bij and hi by A ’ Cgi"(V)Bji and...Studies, 7:511-528. 1975. % ,.%A .48 ൸ Feynman , R. "The Feynman Lectures on Physics". Addison-Wesley. 1963. Gennert, M. &#34A Computational Framework for

  2. Reserve Manpower, Personnel, and Training Research.

    Science.gov (United States)

    1986-09-01

    ucM) q 24 WWESWEEPMR (MCJ/M&#34𔃾 28 MOBLE NHORE UNDERSEA WAAR LHTS 4 ADPICIIA 94PS (LST/LSf) 22 CRAFT OF OPPORTIJT UNITS (COOP) 4 SALVAE SUPS (ARS) 4...Centers with the aid of computer technology . Once decentralized, personnel data would .- be captured, maintained, and reported at the local level. In... Technology Mr. Eugene R. Hall 202/696-4844 Novel Training Systems Center 305/646-4498 ,- CDR George Connor, USU Office of the CNO, Op-091 Dr. Stanley A

  3. Sabot-Projectiles for Cannon

    Science.gov (United States)

    1943-11-01

    model designed for the 20,-^nm Hispano- Suiza cannon. Let Ms be the mass of the sabot in pounds; M . &#34the mass of the subcali- ber projectile in...its projectile. This model xs designed for the 20-mm Hispano- Suiza cannon, but as with all deep-cup sabots tested, does not prove successful in...the 20-mm Hispano- Suiza , for example, the f. maximum pressure is I48OOO lb/in? and for the 37-mm A.T. gun it is • ^0000 lb/in?). V i Attention

  4. Ethics: A Selected Bibliography.

    Science.gov (United States)

    1985-03-01

    of Happiness in the Democratic Creed: An Analysis of Political Ethics. New York: Praeger, 1959. (JC571 V6) 124. Wakin, Malham M., ed. War, Morality...Affairs. New York: St. Mar- tin’s Press, 1979. (JX1255 M58) 219. Plato . Dialogues of Plato , ed. by Justin D. Kaplan. Translated by Ben- jamin Jowett. New...of Ethics." Army, Vol. 34, December 1984, pp. 22-30. 0 60. DeLue, Steven M. &#34 Aristotle , Kant and Rawls on Moral Motivation in a Just Society." American

  5. The Shock and Vibration Digest. Volume 17, Number 1

    Science.gov (United States)

    1985-01-01

    Engrg., 2.1, pp Multistory Frames," IABSE Periodi- 249-263 (1980). ca, 1, pp 141-156 (1980). 22. Bazant , Z.P. and Christensen, M., 34. Noor, A.K. and...CR-171777, 46 pp ( Jan during deployment and/or retrieval of 31, 1984) N84-22598 mass elements leading to time depend- ent inertias. In particular two...83-3125, values of support stiffness are ob- 58 pp ( Jan 1984) AD-A140743 tained by the on-line estimations of 4 rotating angular velocities of a ro

  6. Machine Arithmetic in Residual Classes,

    Science.gov (United States)

    1981-04-03

    1 2 1 1a’<P(3 Proof. Let us consider minimum pseudo-orthogonal numbers M&#34 1 M41’,on basis/ tesi Pt with ranks rp) and r ,, raspectively. SinCF with...inaccuracy .: r-._ Theorem 3.20. (,bout thp sum cf traces). I.n the sta:nardizd/ norma iz system trace S., of number A, which has .A diffren from zero ones...AA and APA can take only values cf 0. 1 an - , h- equality (3.71) is possible only with ~AA1 which coincides with (3.69). Then that in this case AA=I

  7. Study of Raster Metafile Formats.

    Science.gov (United States)

    1985-01-01

    Transfer Standards Virtual Device Metafile (VI)M) Metafiles Raster Reformatting System (RRS) - 20. ABSTRACT (CEm s ot neon and Idenr by block num6).. This...z44 AAAA1 i 6 8 A it* ai. A Ao a,4 -I I V- 40.044 d6 .4. aN. " m 16 uu mus UM 0 0 W 100 o 00 boIs . lw 00 0 ~6 .4 .4. ’. A ..u 1 A U m &#34I 1 .a 0. a4 NM

  8. A Comparative Analysis of Public and Private Sector Graduate Programs in Public Policy

    Science.gov (United States)

    1989-12-01

    Wilson Approved by: 7" ’it: (’’ Nancq CXRobert Tesis Advisor ’R.ichard . ister, ecofd Reader David R. i * ’ rmanDepartment of Administra e enceqes ii...because doctorate degrees are the only postgraduate degrees awarded in public policy. The University of Indiana, also listed in Morgan’s article of top...Examples are "C" ( Doctorate ), "M&#34 (Post Master’s Degree), "Q" (Master’s Degree), and "R" (Significant Experience). Examples of subspecialty codes are

  9. Proceedings of the Annual Precise Time and Time Interval (PTTI) Planning Meeting (6th). Held at U.S. Naval Research Laboratory, December 3-5, 1974

    Science.gov (United States)

    1974-01-01

    laboratories where no weighting procedure is ap- plied, in fact, they do apply a procedure. When the clock stops, it receives the 25 weight zero . And...adjusted around the zero level until switching the polarity selector from positive to negative yields a 5*0 microsecond difference. The positive...Coordination," Proc. IEEE, May 1972. 6. Silva, I. M., Silva, P. M., &#34Coordena^lfo da Hora no Brazil," Publica9äo n? 2 do Servi^ da Hora do Observatorio

  10. Draft Supplement to Final Environmental Statement on Continental United States (CONUS) Over-the-Horizon Backscatter (OTH-B) Radar System, Penobscot, Washington, Somerset Counties, Maine.

    Science.gov (United States)

    1981-08-01

    development of eggs of birds and the pupae of the darkling beetle, Tenebrio molitor , have also been performed with RFR. Although the term uiually refers... Tenebrio Molitor ," Radio Sci., Vol. 14, No. 6S, pp. 165-171 (1979). Greene, F. M., &#34Development of Magnetic Near-Field Probes," U.S. Department of...Liu, L. M., F. J. Rosenbaum, and W. F. Pickard, "The Relation of Teratogenesis in Tenebrio Molitor to the Incidence of Low-Level Microwaves," IEEE

  11. Optimizing Unmanned Aircraft System Scheduling

    Science.gov (United States)

    2008-06-01

    COL_MISSION_NAME)) If Trim( CStr (rMissions(iRow, COL_MISSION_REQUIRED))) <> "" Then If CLng(rMissions(iRow, COL_MISSION_REQUIRED)) > CLng...logFN, "s:" & CStr (s) & " " For iRow = 1 To top Print #logFN, stack(iRow) & "," Next iRow Print #logFN...340" 60 Print #logFN, "m:&#34 & CStr (s) & " " For iRow = 1 To top Print #logFN, lMissionPeriod(iRow

  12. Fabrication of Unidirectional Fiber Reinforced 6061 Aluminum Alloy Using High Pressure Squeeze Casting

    Science.gov (United States)

    1988-12-01

    soiidfication front as a funct:on of t:me. Suoerim csea cni t2s ine are ooin:s tlat reoresent t",e exact solution at the corresoonaing t:mes as cotainea from the...Bomoay, (1981). Nomoto, M., &#34Mechanical Properties of Squeeze Castings in Al- Cu Alloys," Journal Japan Institute Light Metals, Vol. 30 (1980), pp 212-216...5. KaneKo, Y., Murakami, H., Kuroda, K. and Nagazaki, S., "Squeeze Casting of Aluminum," Foundry Trade Journal , Vol. 148 (1980), pp 397-411. 6

  13. Repair of Dam Intake Structures and Conduits: Case Histories

    Science.gov (United States)

    1988-01-01

    II/ 1’~’--·J . ’,,: iT( I I ·lr ! I L h ,. - - ,, h h L __ ..._il~-- .-----~•:s __ .. !:..._ .d......__ -- • !Voncl:;-;;-;., M&#34"’f" ldh IS A...emergency gate and one about 2 ft downstream of the area to be coated. Tlle two baffles were con- nected by a piece of 2-in. polyvinyl chloride ( PVC ...pipe through which water could drain. The upstream baffle would catch the water flowing past the gate and transfer it via the PVC pipe to the

  14. Cedar Grove: An Interdisciplinary Investigation of a Late Caddo Farmstead in the Red River Valley.

    Science.gov (United States)

    1983-10-31

    the Matagorda Bay colony. Fernando del Bosque (Steck 1932). Neither of these In the meantime, in July 1685 the Spanish had learned expeditions...i-5. a. Class D rim designs found on pottery from Cedar Grove; b. Class D body designs CERAMICS 117I Pl amno iF m&#34.no’ El Camino 3 EDorado Eliot I...the variety. It has a single row of diagonally incised lines of rim designs of the El Camino pattern on deep plain on a short rim (our Albertus 2

  15. Lack of inhibition of adhesion of an enteropathogenic Escherichia coli by polycarbophil.

    Science.gov (United States)

    Mack, D R; Blain-Nelson, P L; Mauger, J W

    1993-12-01

    Anionic polyacrylic acid polymers, such as polycarbophil, have a number of properties that would make them suitable carriers for sustained antibiotic release formulations in the intestinal tract. However, little is known with regards to possible microbial adhesion to polycarbophil. The aim of this study was to evaluate for such an interaction using the rabbit enteric pathogen Escherichia coli RDEC-1 (serotype O15:H-). RDEC-1 mediates attaching and effacing binding to intestinal epithelium in a manner morphologically identical to that observed in both human enteropathogenic E. coli and enterohemorrhagic E. coli infections. RDEC-1 bacteria were grown to promote the expression of the mannose-resistant AF/R1 adhesion pili. A nonpiliated mutant, strain M34, was used as a negative control. Using radioactive labeling of bacteria, we quantitated adhesion of piliated RDEC-1 in the presence of polycarbophil using an in vitro adhesion assay system. Binding of piliated RDEC-1 in the adhesion assay was greater than for nonpiliated M34 for all concentrations of bacteria greater than 10(9) (P Polycarbophil did not cause concentration-dependent inhibition of piliated RDEC-1 binding (P > .05). We conclude polycarbophil does not interfere with the AF/R1 adhesin ligand of RDEC-1. Use of this polymer as a mucoadhesive drug delivery vehicle for nonabsorbable antibiotics in the treatment of gastrointestinal infections would not be expected to interfere with the protective effects of intestinal mucins.

  16. Nutrição e crescimento do fungo nematófago Arthrobotrys oligospora

    Directory of Open Access Journals (Sweden)

    Eliane R. Cardoso

    2009-12-01

    Full Text Available As condições de crescimento e os requerimentos nutricionais de Arthrobotrys oligospora, um fungo nematófago, foram investigados em meio líquido. O organismo foi incubado em meio sintético, a 30º C e em cultura estacionária. O perfil da curva de crescimento do fungo ajustou-se a uma equação de 3º grau, mesmo após 15 dias de incubação. A temperatura e o pH ótimos para produção de micélio foram observados a 25º C e pH 5,0, respectivamente. Contudo, não foram observadas diferenças significativas entre a produção de biomassa nas temperaturas de 25º C e 30º C ou pH 5.0 e 6.0. Várias fontes de carbono foram utilizadas pelo fungo, porém a maior produção de biomassa foi verificada com maltose e sacarose. Das fontes de nitrogênio testadas, várias proteínas (triptona, extrato de levedura, caseína, peptona e casaminoácidos e fontes inorgânicas (nitrato de sódio e cloreto de amônio estimularam a maior produção de biomassa. Das várias vitaminas experimentadas, o crescimento do fungo aumentou 2,2 vezes com riboflavina e 2,3 vezes com a mistura biotina e tiamina em relação ao controle, sem vitamina. De modo geral, constatou-se, após o período de incubação, que o pH inicial do meio de cultura pode aumentar até 8,4. Estes resultados sugerem que as variáveis estudadas podem ter papel importante no crescimento do organismo no solo.The growth conditions and the nutritional requirements of Arthrobotrys oligospora, a nematophagous fungus, were investigated in liquid culture. The organism was incubated in a synthetic medium, at 30º C in a static culture. Time course of growth was adjusted to a 3º degree equation, even after 15 days of incubation. Optimal temperature and pH for mycelium production were observed at 25º C and pH 5.0, respectively. However, significant differences were not found among the biomass production at temperatures 25º C and 30º C or pH 5.0 and 6.0. Several carbon sources were used by the fungus

  17. Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy

    Directory of Open Access Journals (Sweden)

    Zueco Jesus

    2009-12-01

    Full Text Available Abstract Background Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1β (IL1β, using the cell wall protein Pir4 as fusion partner. To achieve high cell density and, consequently, high product yield, BY4741 [PIR4-IL1β] was cultured in an aerated fed-batch reactor, using a defined mineral medium supplemented with casamino acids as ACA (auxotrophy-complementing amino acid source. Also the S. cerevisiae mutant BY4741 Δyca1 [PIR4-IL1β], carrying the deletion of the YCA1 gene coding for a caspase-like protein involved in the apoptotic response, was cultured in aerated fed-batch reactor and compared to the parental strain, to test the effect of this mutation on strain robustness. Viability of the producer strains was examined during the runs and a mathematical model, which took into consideration the viable biomass present in the reactor and the glucose consumption for both growth and maintenance, was developed to describe and explain the time-course evolution of the process for both, the BY4741 parental and the BY4741 Δyca1 mutant strain. Results Our results show that the concentrations of ACA in the feeding solution, corresponding to those routinely used in the literature, are limiting for the growth of S. cerevisiae BY4741 [PIR4-IL1β] in fed-batch reactor. Even in the presence of a proper ACA supplementation, S. cerevisiae BY4741 [PIR4-IL1β] did not achieve a high cell density. The Δyca1 deletion did not have a beneficial effect on the overall performance of the strain, but it had a clear effect on its viability, which was not impaired during fed-batch operations, as shown by the kd value (0.0045 h-1, negligible if compared to that of the parental strain (0.028 h-1. However, independently of their robustness, both the parental and the Δyca1 mutant ceased to grow early during fed-batch runs, both strains using most of the

  18. Evaluation of supplementation of sucrose medium on the synthesis of Zymomonas mobilis bio-products = Avaliação da suplementação do meio de sacarose na formação de bio– produtos por Zymomonas mobilis

    Directory of Open Access Journals (Sweden)

    Raquel Renan Jorge Borsari

    2010-07-01

    Full Text Available The effect of the variables pantothenic acid, yeast extract and sodiumchloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.A influência das variáveis: ácido pantotênico, extrato de levedura, cloreto de sódio, e a técnica de permeabilização celular foram investigadas na formação de levana, sorbitol, etanol e biomassa de Zymomonas mobilis utilizando um delineamento estatístico fatorial fracionado 24-1. A biomassa foi determinada por turbidimetria, Os açúcares redutores foram quantificados por Somogy e Nelson, açúcartotal por Fenol Sulfúrico, sorbitol por HPLC e etanol por micro-destilação. A levana produzida foi precipitada com etanol absoluto e determinada como unidade de frutose. Na biossíntese de levana, a variável que mais contribuiu foi a condição celular. Os resultadossugerem que, para a formação da biomassa e etanol, os fatores que mais interferiram foram a concentração de cloreto de sódio e a condição celular que influencia negativamente a produ

  19. 酿酒酵母对弱有机酸胁迫的应激机制研究进展%Research progress on weak organic acid stress mechanism of Saccharomyces cerevisiae

    Institute of Scientific and Technical Information of China (English)

    刘兴艳; 贾博; 赵芳; 王成; 李静援; 战吉宬; 黄卫东

    2013-01-01

    Yeast Saccharomyces cerevisiae is the main microorganism in the production of liquor,but fermentation environment is always disadvantageous to its growth.It is always exposed to several stress conditions including low pH stress resulting from weak organic acid.Yeast cells have developed a common response to environmental adverse conditions,but there are some differences among different stress.Researches on weak organic acid stress mechanism of Saccharomyces cerevisiae in recent years are reviewed in this paper,including gene expression,transcript expression and protein expression.ATP binding cassette transporter Pdrl2 and its transcription factor Warlp,HOG-MAPK pathway,FPS1 aquaglyceroporin,plasma membrane H+-ATPase,superoxide dismutase (SOD),catalase(CAT) and interaction of weak acid stress with other stress are all discussed.We hope it could provide certain reference for better research on weak acid stress mechanism of Saccharomyces cerevisiae.%酿酒酵母是生产酒类产品的主要微生物,而生产中的发酵环境对酿酒酵母来说是一种不利的生长代谢环境,存在多种胁迫因素,其中就包括弱有机酸引起的低pH胁迫.酿酒酵母应对胁迫环境,有自身一套完备的适应机制,不同胁迫应对机制既有相同之处亦有不同之处.本文概括了近年来酿酒酵母对弱有机酸应激机制的研究热点,主要从弱有机酸胁迫的基因表达、转录表达以及蛋白质表达3个水平进行阐述,涉及ATP结合区转运子Pdrl2及其转录因子Warlp,HOG-MAPK途径,水通道蛋白Fpslp,质膜H+-ATP酶,SOD,CAT,以及弱有机酸与其他胁迫的交互作用,旨在为更好地研究酿酒酵母的弱有机酸应激机制提供一定的参考.

  20. Support Resources Demand Parameters - Aircraft. Revision A

    Science.gov (United States)

    1980-01-15

    CONT’D PARAMETER NAME TYPE UNITS Securing Technology (Radome) Scaled Convention: 1 = Hinge and Bolts _: 2 = Hinge and Snap Fastners 3 = Cam Locks Total...wE cn m V> e= (m ,&#34, 11’ m> (A - CA -> -- 2: > 0n u~~~~~~ ’,’ U) Xz:>cm(>to nX : i>a.., t--w, ,anE : a nOan a > -- , a,, n> -j a) , a 4 .) 4, J " 0n...WCJO0tC~ .4EC 000000 4-W~~ 4)W E > o 1= to S- 4- o 00 0 3 d > -U 4- 4- 4- 4- 4.- Ox 00 C - to u* 0 CD 0 M: LC) LC Lo LC) LO U) CAD 1dC DC’ 4v

  1. Examining the relationship between sex and motivation in triathletes.

    Science.gov (United States)

    López-Fernández, Iván; Merino-Marbán, Rafael; Fernández-Rodríguez, Emilio

    2014-08-01

    The present paper assesses the relationship between sex and motivation in triathletes utilising a multidimensional measurement of motivation in sports. Data were collected from 138 triathlon participants (age range 17-72 years): 95 men (M = 36.7 yr., SD = 10.7) and 43 women (M = 34.6 yr., SD = 9.9). Measures included the Sport Motivation Scale, a multi-dimensional measure to assess different types of athletes' motivations for practising their sport. Results showed a significant sex difference in amotivation, with women displaying lower scores; but amotivation scores were very low. The results were not dependent on competition level or age. Men and women competing at the international level in triathlon have similar motivational profiles.

  2. [Microsatellite data verify low genetic differentiation between western and eastern subspecies of the common crane Grusgrus L. (Gruidae, Aves)].

    Science.gov (United States)

    Mudrik, E A; Kashentseva, T A; Redchuk, P S; Politov, D V

    2015-01-01

    Using a set of 10 microsatellite loci (Gram-22, Gram-30, Gpa-12, Gpa-38, Gpa-39, Gj-M15, Gj-M34, Gj-4066, Gj-8077, Gj-2298) a high level of genetic variability (N(A) = 10.2, H(O) = = 0.684, H(E) = 0.728) and low genetic differentiation (F(ST) = 0.011) in the Common crane (Grus grus L.) was detected throughout its range. Genetic diversity in disputed western (G. g. grus) and eastern (G. g. lilfordi) sub- species was shown to be similar. Spatial distribution of multi-locus individual genotypes has not been revealed (R(XY) = 0.017). Despite low differentiation, subspecies and local populations of the Common crane should be considered as separate conservational units. Organization of programs for gene pool conservation and monitor- ing requires more detailed analysis based on combined analysis of various molecular markers.

  3. All Prime Contract Awards by State or Country, Place, and Contractor. Part 14 (Akron, New York-Wilson, North Carolina)

    Science.gov (United States)

    1990-01-01

    01 0 04 211 L) m4 I (O40 4 00 000 0000 0 0 0 10 L) (0 (-4N( H 00CA0100 0 0 00 0 0 0a U4(-4N( Haz z z 00000 0 > 0 000. 0 0 0 01 0> 0z a I1 1 (0-4N(0 If...M&#34 ION 0- C’ 7) x 4-4 <- W0 w 0 < w z a0 ":44000 w443j i ::0 Ix ZUL - 4c 00 0 In t- .1J 2 0 (-40 cr I M0-4 4f 0 C w 0 0 (A C/L0 0 Q 0-44 14CO-4 442 0 4

  4. The USAF STINFO Program Overview

    Science.gov (United States)

    1989-06-01

    For N’IT T I S J ),.-t lf•i cation U ’Dstri:’... Ion/ A :.’ja I tY Codes ii r 2/or IDist SpOc al I/ - I 4318 essend noSteN INFO/tek 4318...accessible online as part I of the DROLS system. " STINFO responsibilities concerning Work Units are: m &#34 Ensure that the R&T WUIS database is searched...System Database" i 5. DLAM 4185.18, "Defense RDT&E Online System, Dial-Up Retrieval Self-Training Manual" I I I I ’I I l I I I I USAF STINFO Ovwrview

  5. Worldwide U.S. Active Duty Military Deaths, Alphabetical Index by Name. Part 2 (LE BLANK WENDELL JOHN - ZYSK CAROL ROSE)

    Science.gov (United States)

    1991-01-01

    Z aZ w _1-- 3 ix u . LU w3 .<C ZUJx iZwO W(0 0 w4w-t I-- o:~ -i w z z w .I-mo 3c w4>0EI- ’Wi 1- :0 N 02~ W 0 I (0 w u -i~~~~~~~~ -i ac . ŗ O 00 1D : w...iw c 0. & -in2z WZ(D x > WP-,1Ř"-" 0-1 (A0>- WU ezw m 0 :3LU -i n > m D W- C /)0Z - W Z: >0 < 14 cc "- < < w w x m &#34 w w 0--- U 4Z Ŕ O -l, w 4- -L

  6. Laser diagnostics of native cervix dabs with human papilloma virus in high carcinogenic risk

    Science.gov (United States)

    Peresunko, O. P.; Karpenko, Ju. G.; Burkovets, D. N.; Ivashko, P. V.; Nikorych, A. V.; Yermolenko, S. B.; Gruia, Ion; Gruia, M. J.

    2015-11-01

    The results of experimental studies of coordinate distributions of Mueller matrix elements of the following types of cervical scraping tissue are presented: rate- low-grade - highly differentiated dysplasia (CIN1-CIN3) - adenocarcinoma of high, medium and low levels of differentiation (G1-G3). The rationale for the choice of statistical points 1-4 orders polarized coherent radiation field, transformed as a result of interaction with the oncologic modified biological layers "epithelium-stroma" as a quantitative criterion of polarimetric optical differentiation state of human biological tissues are shown here. The analysis of the obtained Mueller matrix elements and statistical correlation methods, the systematized by types studied tissues is accomplished. The results of research images of Mueller matrix elements m34 for this type of pathology as low-grade dysplasia (CIN2), the results of its statistical and correlation analysis are presented.

  7. Development of a Computer Program for Store Airloads Prediction Technique

    Science.gov (United States)

    1976-10-01

    II US 1 to t118 11 t l Id 81 1.) 8,11’o -818,I itIl M&#34 .,8𔃻 . !. ’ll w .1, w w I!, . W8- 4 It 𔃺 ,4 ’D "’ 00C3 . , 0 ,lt")t C, 4 2 S . N 4 N8\\N IA...iwwUI1 k)" 1 A V)a. n% 40I 0INN11 2C ,1 , r I)I n- 2o " I 4 1 w D ( N o=i A , Ii1 0 ,N O"N 1 - NI Io -I NIL 4e ; 𔃺 o 4i n ,O 1I A 2’ f# "tl 20000 -, aa

  8. Transparent Polyolefin Film Armor

    Science.gov (United States)

    1981-08-01

    Routing is then accomplished with an 18,000 RPM hand held router using a 0.500 inch diameter, two flute carbide inlaid router bit. As long as the... router bit was kept sharp, there was no delarnination. 1" " I ROUTER COLLAR M&#34 ,OLDED POLYOLEFIN _ 0,25 ACRYLIC 0,500 DIAM ETERROUTER BIT PRESSURE AREA...F-) LL- 0) 00 0-(N ) C0 co C cnc ) *(NJ 0) Cl C) 00. m) m -c 0) CC r~ )- 0~0 4-- C:. :3 (v 40 U) a) CL Lu CL E t,4- ’.) -x E E a) N~E -C0 0) 4- 0 C

  9. New cytotoxic phloroglucinols, baeckenones D-F, from the leaves of Indonesian Baeckea frutescens.

    Science.gov (United States)

    Nisa, Khoirun; Ito, Takuya; Kodama, Takeshi; Tanaka, Masami; Okamoto, Yasuko; Asakawa, Yoshinori; Imagawa, Hiroshi; Morita, Hiroyuki

    2016-03-01

    Three new phloroglucinols, baeckenones D-F (1-3), were obtained from the leaves of Indonesian Baeckea frutescens, along with the known unusual endoperoxide, phloroglucinol (4). The structures of the isolated compounds were elucidated by 1D and 2D NMR and HREIMS spectra. Furthermore, the stereochemistry of baeckenone D (1) was established by an X-ray diffraction analysis. Among the isolated compounds 1-4, baeckenone F (3) showed moderate cytotoxic activities against human pancreatic (PSN-1), lung (A549), and breast (MDA-MB-231) cancer cell lines, with IC50 values of 33.3 μM, 34 μM, and 39.3 μM, respectively.

  10. Strong earthquake activity all over the world and strong-moderate earthquake activity within and near China (June, 2001 ~ July, 2001)

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@Illustration All the data in this catalog are chosen from the 2Preliminary Seismological Report of Chine-se Seismic Stations2 (Its abbreviation is 2Monthly Report2). The catalog includes the events of M34.7 in and near China and M36 all over the world. The 2Monthly Report2 is monthly compiled by the Ninth Section of Institute of Geophysics, CSB. The origin times of earthquakes in the catalog adopt coordinated universal time (UTC) in accordance with international convention. The location of every epicenter is expressed by its latitude and longitude, at the same time by the corresponding geographical region proposed by Flinn and Engdahl. The regional names are only for references and do not imply any political significance. The number of stations used and standard deviation are also given in order to illus-trate the precision of location.

  11. Atitudes da população portuguesa em relação à homoparentalidade

    Directory of Open Access Journals (Sweden)

    Pedro Alexandre Costa

    2013-12-01

    Full Text Available O objetivo deste estudo foi avaliar as atitudes da população portuguesa em relação à homoparentalidade. Participaram 993 pessoas com idades entre os 18 e os 69 anos (M=34; DP=11, respondendo a um questionário que incluiu uma vinheta descrevendo um casal heterossexual, um casal de lésbicas ou um casal gay que pretendia adotar. Foi pedido aos participantes que avaliassem os casais enquanto futuros pais, e se antecipavam dificuldades emocionais e sociais no desenvolvimento da criança. Apesar da maioria dos participantes apresentar uma atitude favorável à homoparentalidade, os resultados revelaram uma atitude mais favorável em relação ao casal heterossexual. Os participantes anteciparam mais problemas emocionais e sociais nas crianças adotadas por um casal de duas mulheres, e mais ainda por um casal de dois homens.

  12. MOPADS (Models of Operator Performance in Air Defense Systems). Appendices

    Science.gov (United States)

    1984-11-01

    acL me C2 - at 441 g* m I r...aI U. a SL I 0 I I - .b caa -4 I I ~ a I aIa ~ a SJ-54 * 0 1 *a 4c I 6b= ft acL - C) a cc I- I c iI C6 6 a66A 1 6 m I i 3 I I I to I ac W Li i I ’a Q6...8217 I I ,," aqa m I. Nopm:. a Or- ac & meI o II•Ir, 6. Z;J ow, 01 -- " C. a: 111 0, 1•-Ja %1 - 0 9 n m &#34’ ý& 0 1 0 Ofa guia U 4q%. I I •i to j S. C 0-

  13. Dynamics of Liquid-Filled Projectiles

    Science.gov (United States)

    1976-04-01

    abttract entered In Block 20, It dlllarant Irom Report) "’. SUPPLEMENTARY NOTES KEY WORDS (Continue on ravarae aide It naceaaary and Identify by...Ammunition development XM736 ABSTRACT (Continue on ravarae aide It naeaaaary tfd Identify by ’ ’ock number) ’•This report presents a collection of analytic...icm (VJ m «© •o-#m^)co*|( viH o «c cr K K co.o ** m &#34~’-» co >o o co cr i • cr omoK|crco -4 -• i* o <o n I’M or H o mmccmcvjocom-omin m K K

  14. Prime Contract Awards Alphabetically by Contractor, by State or Country, and Place. Part 16 (Panhandle Food Sales-Ray Gene Electric Co Inc)

    Science.gov (United States)

    1990-01-01

    AD-A236 466 1990 ANNUAL PRIME CONTRACTS AWARDS ALPHABETICALLY BY CONTRACTOR, BY STATE OR COUNTRY, AND PLACE. PT-16 (PANHANDLE FOOD SALES-RAY GENE ...4 I-l e~ I 0 MM 0)1 47470:14710) 0) 0) 0) I" gene , I LA 4 00 0) co -4 -FF- F- C (D <r- 0CD I~ gene , U0 -4 0. C4 0> 0D-4-4-40 C> C- N0 -4 (n L-4 cn...LW ,U ’L a) I CIme 𔃺" 0)0)100)0)10)0) (00 0 Ei(Cn" cc cc c c c c c c c c c 410mc’ " ::U. C 001. C00 r-0C0C r00 C w izI0’ M&#34 ~ 000000 , . > .0 000 s-s

  15. Study of Sources in AFGL Rocket Infrared Study.

    Science.gov (United States)

    1980-02-07

    j14 4 03 0%m N0 C f)e~ NC’ U 0 .. C t 0 % -gN A)~ NIf Ncc0 N(Nf~C I)0)C. 0.- ~0 NIf N’- x r- In Ur) In N e’j 0 1! I I t I I I 0 , r’( co r- LnU)L...iK21II III I I I I I I 2~~ oe M3 I ~* ~M8: SRb S .: -9 : M La . .... K2 I a.. .. 3 1 ..-..... 0 . "-.,,...%. e *OP .* C.4(:SR •. •C5- 6 : M &#34. ’. C6 . I M

  16. Standard Operating Procedure

    Science.gov (United States)

    1997-01-01

    l^m .:#,&#34■• y’’:>’£l’S ABO GROUP CONFIRMED |[ Rh OF .NEGATIVE UNITS CONFIRMED fe^/w’H’o’^Ä^ ■ •:.■.■:•• •■’ .Tv-v^l^.>’-V.^^V’ir;i-.^K-V.^’vV-i...using 0.02 ml of whole blood diluted with 5.98 ml of Drabkin’s reagent (1:251 dilution). 51 I. Measure the supernatant hemoglobin concentration...34NcUiod fef*ɜ 17-0? STANDARD OPERATING PROCEDURE Red Blood Cells Collected in the CPDA-1 800 ml Primary PVC Plastic Bag Collection System and

  17. Performance of 3-cell Seamless Niobium cavities

    Energy Technology Data Exchange (ETDEWEB)

    Kneisel, Peter K. [JLAB; Ciovati, Gianluigi [JLBA; Jelezov, I. [DESY, Hamburg; Singer, W. [DESY, Hamburg; Singer, X. [DESY, Hamburg

    2009-11-01

    In the last several months we have surface treated and cryogenically tested three TESLA-type 3-cell cavities, which had been manufactured at DESY as seamless assemblies by hydroforming. The cavities were completed at JLab with beam tube/flange assemblies. All three cavities performed very well after they had been post-purified with titanium at 1250C for 3 hrs. The cavities, two of which consisted of an end cell and 2 center cells and one was a center cell assembly, achieved gradients of Eacc = 32 MV/m, 34 MV/m and 35 MV/m without quenches. The performance was limited by the appearance of the “Q-drop” in the absence of field emission. This contribution reports about the various measurements undertaken with these cavities.

  18. Polarization lidars with conical scanning for retrieving the microphysical characteristics of cirrus clouds

    Science.gov (United States)

    Konoshonkin, Alexander V.; Borovoi, Anatoli G.; Liu, Dong; Wang, Zhenzhu; Balin, Yuri S.; Kustova, Natalia V.; Kokhanenko, Grigorii; Penner, Iogannes; Nasonov, Sergey V.; Bryukhanov, Ilia D.; Doroshkevich, Anton A.

    2015-11-01

    The paper presents the first results of observations of cirrus clouds by polarization lidars with conical scanning, which were developed in Hefei (China) and in Tomsk (Russia). The light scattering matrix of ice crystal particles of cirrus clouds has been calculated for the first by the authors within the framework of the physical optics approximations in the case of conical scanning lidar. It is found that in this case the Mueller matrix consists of ten non-zero elements, four of which are small and can't be applied to interpret the azimuthal distribution of particle orientation. All the diagonal elements have a strong azimuthal dependence. Among the off-diagonal elements only one element M34 carries additional information for interpreting the azimuthal distribution.

  19. A Higgs boson candidate decaying into 4 muons in the ATLAS detector

    CERN Multimedia

    ATLAS Collaboration

    2012-01-01

    Event display of a 4mu candidate. EventNumber: 71902630 RunNumber: 204769 m4ell=125.1 GeV. m12=86.3 GeV, m34=31.6 GeV. mu1: pT,eta,phi=36.1 GeV, 1.29, 1.33 . mu2: pT,eta,phi=47.5 GeV, 0.69, -1.65 . mu3: pT,eta,phi=26.4 GeV, 0.47, -2.51 . mu4: pT,eta,phi=71.7 GeV, 1.85, 1.65 . pT4ell= 27.0 GeV. ETmiss=41.8 GeV. (ATLAS-CONF-2012-169)

  20. Personal self-concept and satisfaction with life in adolescence, youth and adulthood.

    Science.gov (United States)

    Goñi Palacios, Eider; Esnaola Echaniz, Igor; Rodríguez Fernández, Arantzazu; Camino Ortiz de Barrón, Igor

    2015-01-01

    The main aim of this study was to explore the relationships between personal self-concept and satisfaction with life, with the latter as the key indicator for personal adjustment. The study tests a structural model which encompasses four dimensions of self-concept: self-fulfillment, autonomy, honesty and emotions. The 801 participants in the study, all of whom were aged between 15 and 65 (M = 34.03, SD = 17.29), completed the Satisfaction with Life Scale (SWLS) and the Personal Self-Concept (APE) Questionnaire. Although the four dimensions of personal self-concept differ in their weight, the results show that, taken together, they explain 46% of the differences observed in satisfaction with life. This implies a weight that is as significant as that observed for general self-esteem in previous research studies. This issue should be dealt with early on, during secondary education, in order to help prevent psychological distress or maladjustment.

  1. Dual-Modality Prostate Imaging with PET and Transrectal Ultrasound

    Science.gov (United States)

    2009-04-01

    Z(>>(*B(Z( JDL (*BD(6?&(’&::#90*#:#$&N(R&:8"<%(*’:<%N&$( ?8@(8(L(*B(NɠB&"&$D(\\=](V&*#%@"$Q*"&N(*#$#%8:(4S6(<B8R&(#M(&#34?&(4S60Q:"$8@#Q%N(A?8%"#BD(6...8[Q80*#:#$&N( $&*Ŝ%RQ:8$(A&:F<@(?8@(#Q"&$(N<B&%@<#%@(#M(>G(*B(Z(>>(*B(Z( JDL (*BD(6?&(N8$+(R$&&%0*#:#$&N( *’:<%N$<*8:(A$#@Ŝ"&(?8@(8(L(*B(NɠB&"&$(8%N

  2. Mueller matrix polarimetry for characterizing microstructural variation of nude mouse skin during tissue optical clearing.

    Science.gov (United States)

    Chen, Dongsheng; Zeng, Nan; Xie, Qiaolin; He, Honghui; Tuchin, Valery V; Ma, Hui

    2017-08-01

    We investigate the polarization features corresponding to changes in the microstructure of nude mouse skin during immersion in a glycerol solution. By comparing the Mueller matrix imaging experiments and Monte Carlo simulations, we examine in detail how the Mueller matrix elements vary with the immersion time. The results indicate that the polarization features represented by Mueller matrix elements m22&m33&m44 and the absolute values of m34&m43 are sensitive to the immersion time. To gain a deeper insight on how the microstructures of the skin vary during the tissue optical clearing (TOC), we set up a sphere-cylinder birefringence model (SCBM) of the skin and carry on simulations corresponding to different TOC mechanisms. The good agreement between the experimental and simulated results confirm that Mueller matrix imaging combined with Monte Carlo simulation is potentially a powerful tool for revealing microscopic features of biological tissues.

  3. Capabilities of Multiplicative Array Processors as Signal Detector and Bearing Estimator

    Science.gov (United States)

    1974-12-31

    M) u (o/n; cm). (4-47) Then, using (D-9) and recalling that e = 1MB sin a, we find a(y) = iarccos 1 48 ß2(4M2-l) \\/-T( J7t!-c) VMH W c...8217)2+(2M-l) tD £](B-CcosM3sina). (5-22) B-r Therefore, an estimate a(w; a) based on the two array sums has an error bound, Var(i) > (B2-C2)[|5E(a)] 2...If^L &$L exp[-4-4MH 2D2(2-cos2e)](SI)2. (5-50) ßV4M&#34-i I+4M* TD * 5.3 COMPUTED RESULTS Computations of the various bounds on estimator error

  4. Large Signal Time Dependent Quantum Mechanical Transport in Quantum Phase Based Devices

    Science.gov (United States)

    1994-06-10

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  5. Handbook on Materials for Superconducting Machinery, Includes Data Sheets for First and Second Supplements, November 1975 and January 1977

    Science.gov (United States)

    1977-01-01

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  6. Mathematical Modelling of Waveguiding Techniques and Electron Transport. Volume 2.

    Science.gov (United States)

    1984-01-01

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  7. Department of the Navy Amended FY 1992/FY 1993 Biennial Budget Estimates: Justification of Estimates Submitted to Congress January 1992: Operation and Maintenance, Navy. Book 3. Budget Activity 8: Training, Medical and Other General Personnel Activities. Budget Activity 9: Administration and Associated Activities. Budget Activity 10: Support of Other Nations

    Science.gov (United States)

    1992-01-01

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  8. Standardized UXO Technology Demonstration Site Moguls Scoring Record Number 588 (Parsons)

    Science.gov (United States)

    2005-06-01

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  9. Measuring premorbid IQ in traumatic brain injury: an examination of the validity of the Wechsler Test of Adult Reading (WTAR).

    Science.gov (United States)

    Green, Robin E A; Melo, Brenda; Christensen, Bruce; Ngo, Le-Anh; Monette, Georges; Bradbury, Cheryl

    2008-02-01

    Estimation of premorbid IQ in traumatic brain injury (TBI) is clinically and scientifically valuable because it permits the quantification of the cognitive impact of injury. This is achieved by comparing performances on tests of current ability to estimates of premorbid IQ, thereby enabling current capacity to be interpreted in light of preinjury ability. However, the validity of premorbid IQ tests that are commonly used for TBI has been questioned. In the present study, we examined the psychometric properties of a recently developed test, the Wechsler Test of Adult Reading (WTAR), which has yet to be examined for TBI. The cognitive performance of a group of 24 patients recovering from TBI (with a mean Glasgow Coma Scale score in the severely impaired range) was measured at 2 and 5 months postinjury. On both occasions, patients were administered three tests that have been used to measure premorbid IQ (the WTAR and the Vocabulary and Matrix Reasoning subtests of the Wechsler Adult Intelligence Scale 3rd Edition, WAIS-III) and three tests of current ability (Symbol Digit Modalities Test-Oral and Similarities and Block Design subtests of the WAIS-III). We found that performance significantly improved on tests of current cognitive ability, confirming recovery. In contrast, stable performance was observed on the WTAR from Assessment 1 (M = 34.25/50) to Assessment 2 (M = 34.21/50; r = .970, p tests are indicated (i.e., in patients for whom English is spoken and read fluently), these results endorse the use of the WTAR for patients with TBI.

  10. The Mars Science Laboratory Curiosity rover Mastcam instruments: Preflight and in-flight calibration, validation, and data archiving

    Science.gov (United States)

    Bell, James F.; Godber, A.; McNair, S.; Caplinger, M.A.; Maki, J.N.; Lemmon, M.T.; Van Beek, J.; Malin, M.C.; Wellington, D.; Kinch, K.M.; Madsen, M.B.; Hardgrove, C.; Ravine, M.A.; Jensen, E.; Harker, D.; Anderson, Ryan; Herkenhoff, Kenneth E.; Morris, R.V.; Cisneros, E.; Deen, R.G.

    2017-01-01

    The NASA Curiosity rover Mast Camera (Mastcam) system is a pair of fixed-focal length, multispectral, color CCD imagers mounted ~2 m above the surface on the rover's remote sensing mast, along with associated electronics and an onboard calibration target. The left Mastcam (M-34) has a 34 mm focal length, an instantaneous field of view (IFOV) of 0.22 mrad, and a FOV of 20° × 15° over the full 1648 × 1200 pixel span of its Kodak KAI-2020 CCD. The right Mastcam (M-100) has a 100 mm focal length, an IFOV of 0.074 mrad, and a FOV of 6.8° × 5.1° using the same detector. The cameras are separated by 24.2 cm on the mast, allowing stereo images to be obtained at the resolution of the M-34 camera. Each camera has an eight-position filter wheel, enabling it to take Bayer pattern red, green, and blue (RGB) “true color” images, multispectral images in nine additional bands spanning ~400–1100 nm, and images of the Sun in two colors through neutral density-coated filters. An associated Digital Electronics Assembly provides command and data interfaces to the rover, 8 Gb of image storage per camera, 11 bit to 8 bit companding, JPEG compression, and acquisition of high-definition video. Here we describe the preflight and in-flight calibration of Mastcam images, the ways that they are being archived in the NASA Planetary Data System, and the ways that calibration refinements are being developed as the investigation progresses on Mars. We also provide some examples of data sets and analyses that help to validate the accuracy and precision of the calibration

  11. The Mars Science Laboratory (MSL) Mast cameras and Descent imager: Investigation and instrument descriptions

    Science.gov (United States)

    Malin, Michal C.; Ravine, Michael A.; Caplinger, Michael A.; Tony Ghaemi, F.; Schaffner, Jacob A.; Maki, Justin N.; Bell, James F.; Cameron, James F.; Dietrich, William E.; Edgett, Kenneth S.; Edwards, Laurence J.; Garvin, James B.; Hallet, Bernard; Herkenhoff, Kenneth E.; Heydari, Ezat; Kah, Linda C.; Lemmon, Mark T.; Minitti, Michelle E.; Olson, Timothy S.; Parker, Timothy J.; Rowland, Scott K.; Schieber, Juergen; Sletten, Ron; Sullivan, Robert J.; Sumner, Dawn Y.; Aileen Yingst, R.; Duston, Brian M.; McNair, Sean; Jensen, Elsa H.

    2017-08-01

    The Mars Science Laboratory Mast camera and Descent Imager investigations were designed, built, and operated by Malin Space Science Systems of San Diego, CA. They share common electronics and focal plane designs but have different optics. There are two Mastcams of dissimilar focal length. The Mastcam-34 has an f/8, 34 mm focal length lens, and the M-100 an f/10, 100 mm focal length lens. The M-34 field of view is about 20° × 15° with an instantaneous field of view (IFOV) of 218 μrad; the M-100 field of view (FOV) is 6.8° × 5.1° with an IFOV of 74 μrad. The M-34 can focus from 0.5 m to infinity, and the M-100 from 1.6 m to infinity. All three cameras can acquire color images through a Bayer color filter array, and the Mastcams can also acquire images through seven science filters. Images are ≤1600 pixels wide by 1200 pixels tall. The Mastcams, mounted on the 2 m tall Remote Sensing Mast, have a 360° azimuth and 180° elevation field of regard. Mars Descent Imager is fixed-mounted to the bottom left front side of the rover at 66 cm above the surface. Its fixed focus lens is in focus from 2 m to infinity, but out of focus at 66 cm. The f/3 lens has a FOV of 70° by 52° across and along the direction of motion, with an IFOV of 0.76 mrad. All cameras can acquire video at 4 frames/second for full frames or 720p HD at 6 fps. Images can be processed using lossy Joint Photographic Experts Group and predictive lossless compression.

  12. Optimization of Fermentation Medium for Type b Haemophilus influenzae%b型流感嗜血杆菌发酵培养基的优化

    Institute of Scientific and Technical Information of China (English)

    袁玉兰; 郭京蓉; 高华; 周继唯; 王立新; 林云

    2011-01-01

    batches of Hib were fermented by using the selected medium and further determined for the protein and nucleic acid contents in polysaccharide complex. Results The protein and nucleic acid contents in fermentation supematant using various media were different, which were low and stable in those using medium No. 2 mainly consisting of casamino acid powder and yeast extract powder. The additives in medium showed different effects on the morphology, color of fermentation supernatant as well as recovery, quality and property of polysaccharide complex. The fermentation supernatant of Hib using medium b was of good morphology as well as high recovery and quality of polysaccharide complex.In the three batches of fermentation liquids, about 7% ~ 10% of proteins and 34% ~ 42% of nucleic acids were precipitated into the polysaccharide complex. Conclusion Medium No. 2 was selected as basal medium, and medium b as fermentation medium for Hib.The study provided an experimental basis for optimization of medium and purification of polysaccharide antigen of Hib vaccine.

  13. Mechanism of Excretion of a Bacterial Proteinase: Factors Controlling Accumulation of the Extracellular Proteinase of a Sarcina Strain (Coccus P)

    Energy Technology Data Exchange (ETDEWEB)

    BISSELL, MINA J.; TOSI, ROBERTO; GORINI, LUIGI

    1970-06-29

    +} was needed for enzyme synthesis or excretion (1) was challenged when the observation was made (5) that Ca{sup 2+} and not Mn{sup 2}, Mg{sup 2+}, Sr{sup 2+}, or Ba{sup 2+} was needed for preventing irreversible loss of activity of several bacterial proteinases. In particular, in the case of the excreted proteinase of Coccus P, it was shown (17) that this irreversible inactivation is due to autodigestion occurring in the absence of Ca2 . An antiwetting agent, Ficoll, delays this autodigestion, suggesting that the function of Ca{sup 2+} is to stabilize an already active form of the enzyme molecule rather than to act as a constituent of the prosthetic group required for activity. It has also been observed that, when Coccus P is grown in a complex proteose peptone medium, the proteinase appears abruptly late in the growth of the culture. The sudden burst of activity was explained by demonstrating the presence of a zymogen which is activated autocatalytically (8). The late appearance of activity was accounted for when it was discovered that in minimal medium containing Ca{sup 2+}, Coccus P excreted the proteinase immediately at the onset of growth (9), but that addition of Casamino Acid hydrolysate delayed enzyme production for a length of time roughly proportional to the amount added (H. Ennis and L. Gorini, 1959, unpublished data). A similar amino acid effect was observed for other proteolytic bacteria (3, 13). It was assumed, therefore, that in the absence of amino acids an unrestricted proteinase production could be found. However, another deviation, from a constant relationship between amount of enzyme and amount of cells producing it, became evident by using minimal medium. The rate of accumulation of enzyme decreased gradually, long before exponential growth had slowed down (T. Heyman and L. Gorini, 1955, unpublished data). As yet, no explanation for this decline has been provided. In this paper, in addition to studying the role of Ca{sup 2+} in enzyme production, we

  14. A color-period diagram for the open cluster M 48 (NGC 2548), and its rotational age

    Science.gov (United States)

    Barnes, Sydney A.; Weingrill, Joerg; Granzer, Thomas; Spada, Federico; Strassmeier, Klaus G.

    2015-11-01

    Rotation periods are increasingly being used to derive ages for cool single field stars. Such ages are based on an empirical understanding of how cool stars spin down, acquired by constructing color-period diagrams (CPDs) for a series of open clusters. Our main aims here are to construct a CPD for M 48, to compare this with other clusters of similar age to check for consistency, and to derive a rotational age for M 48 using gyrochronology. We monitored M 48 photometrically for over 2 months with AIP's STELLA I 1.2 m telescope and the WiFSIP 4K imager in Tenerife. Light curves with 3 mmag precision for bright (V ~ 14 mag) stars were produced and then analysed to provide rotation periods. A cluster CPD has then been constructed. We report 62 rotation periods for cool stars in M 48. The CPD displays a clear slow/I-sequence of rotating stars, similar to those seen in the 625 Myr-old Hyades and 590 Myr-old Praesepe clusters, and below both, confirming that M 48 is younger. A similar comparison with the 250 Myr-old M 34 cluster shows that M 48 is older and does not possess any fast/C-sequence G or early K stars like those in M 34, although relatively fast rotators do seem to be present among the late-K and M stars. A more detailed comparison of the CPD with rotational evolution models shows that the cluster stars have a mean age of 450 Myr, and its (rotating) stars can be individually dated to ± 117 Myr (26%). Much of this uncertainty stems from intrinsic astrophysical spread in initial periods, and almost all stars are consistent with a single age of 450 Myr. The gyro-age of M 48 as a whole is 450 ± 50 Myr, in agreement with the previously determined isochrone age of 400 ± 100 Myr. Based on data obtained with the STELLA robotic telescopes in Tenerife, an AIP facility jointly operated by AIP and IAC; this paper presents results for the STELLA Open Cluster Survey (SOCS).Appendices A and B are available in electronic form at http://www.aanda.orgThe cluster photometry

  15. Phylogeographic Analysis of Haplogroup E3b (E-M215) Y Chromosomes Reveals Multiple Migratory Events Within and Out Of Africa

    Science.gov (United States)

    Cruciani, Fulvio; La Fratta, Roberta; Santolamazza, Piero; Sellitto, Daniele; Pascone, Roberto; Moral, Pedro; Watson, Elizabeth; Guida, Valentina; Colomb, Eliane Beraud; Zaharova, Boriana; Lavinha, João; Vona, Giuseppe; Aman, Rashid; Calì, Francesco; Akar, Nejat; Richards, Martin; Torroni, Antonio; Novelletto, Andrea; Scozzari, Rosaria

    2004-01-01

    We explored the phylogeography of human Y-chromosomal haplogroup E3b by analyzing 3,401 individuals from five continents. Our data refine the phylogeny of the entire haplogroup, which appears as a collection of lineages with very different evolutionary histories, and reveal signatures of several distinct processes of migrations and/or recurrent gene flow that occurred in Africa and western Eurasia over the past 25,000 years. In Europe, the overall frequency pattern of haplogroup E-M78 does not support the hypothesis of a uniform spread of people from a single parental Near Eastern population. The distribution of E-M81 chromosomes in Africa closely matches the present area of distribution of Berber-speaking populations on the continent, suggesting a close haplogroup–ethnic group parallelism. E-M34 chromosomes were more likely introduced in Ethiopia from the Near East. In conclusion, the present study shows that earlier work based on fewer Y-chromosome markers led to rather simple historical interpretations and highlights the fact that many population-genetic analyses are not robust to a poorly resolved phylogeny. PMID:15042509

  16. Experiência do estudante de semiologia médica em aulas práticas com o paciente à beira do leito

    Directory of Open Access Journals (Sweden)

    Rilva Lopes de Sousa-Muñoz

    Full Text Available O objetivo deste estudo foi identificar as percepções de estudantes de Medicina que cursaram a disciplina de Semiologia Médica em relação ao contato com os pacientes à beira do leito em aulas práticas no Hospital Universitário Lauro Wanderley, da Universidade Federal da Paraíba. Pesquisa observacional e descritiva com entrevistas por meio de autoaplicação de questionário semiestruturado elaborado pelos autores e pré-testado. Participaram do estudo 105 alunos com idade entre 20 e 24 (21 ± 1,1 anos, 56,2% do sexo masculino. A grande maioria declarou dificuldade na abordagem clínica inicial do doente (89/84,8% e 72 (68,6% referiram insegurança ou medo. Houve aumento de respostas positivas em momentos posteriores da disciplina, porém 34,3% ainda reportaram sentimentos negativos. Observou-se maior constrangimento no questionamento do paciente sobre temas mais íntimos por alunos do sexo masculino. Esses achados reforçam a necessidade de valorizar questões éticas, humanísticas e psicopedagógicas no início da graduação nas escolas médicas.

  17. Hepatitis C virus antibodies in high risk juvenile onset systemic lupus erythematosus.

    Science.gov (United States)

    Aikawa, Nádia E; Nascimento, Ana P; Hayata, André L S; Bonfá, Eloisa; Goldenstein-Schainberg, Cláudia

    2016-01-01

    To evaluate the prevalence of hepatitis C virus (HCV) infection in high risk juvenile systemic lupus erythematosus (JSLE). Forty low income JSLE patients (6M:34F; mean age 19±4.4 yrs; mean disease duration 6±3.2 yrs) were studied. Twenty healthy children and adolescents matched for social economical level were included as controls. Anti-HCV tests were performed using a third generation microparticle enzyme immunoassay. Inclusion criterion was low social economical level. The frequencies of anti-HCV antibody were low and comparable between JSLE and control group (2.5% vs. 0, p=1.0). JSLE patients had significantly more risk factors for HCV infection compared to the control group, including immunosuppressive treatment (90% vs. 0, p<0.0001), hospitalization (50% vs. 12.5%, p=0.0006) and invasive procedures (47.5% vs. 12.5%, p=0.001). The observed low frequency of anti-HCV antibodies in high risk JSLE suggests that this virus does not seem to have a relevant role in the pathogenesis of this disease. Copyright © 2016 Elsevier Editora Ltda. All rights reserved.

  18. A leitura no ensino médio e o uso das estratégias metacognitivas

    Directory of Open Access Journals (Sweden)

    Janete Aparecida da Silva Marini

    2014-04-01

    Full Text Available O presente estudo teve por objetivo identificar a freqüência de uso das estratégias metacognitivas de leitura por estudantes do Ensino Médio, verificando a influência das variáveis idade, gênero, série, turno e rede de ensino freqüentada. Participaram 641 estudantes de escolas públicas e particulares, com idade entre 14 e 17 anos.  O instrumento utilizado foi a Escala de Estratégias Metacognitivas de Leitura. A análise dos resultados revelou que as estratégias metacognitivas de leitura do tipo solução de problemas são as mais utilizadas (M=15,38; DP=4,78 entre os participantes.  Os estudantes revelaram usar com mais freqüência as estratégias metacognitivas no durante a leitura (M=26,81; DP=7,91. Verificou-se que as estudantes do sexo feminino fazem mais uso dessas (M=41,12 e DP =11,28 que os estudantes do sexo masculino (M=34,12 e DP= 10,83. Constatou-se diferenças quanto a, idade, rede de ensino, série e turno freqüentado.

  19. Source Header List. Volume 1. A through K

    Science.gov (United States)

    1998-07-01

    MU Mu mc M0M z mu LL Z.J Z>-= Ewz EzW EEw E4 EzIn E Mw E Eu.- EO E K(n 0 4Eu. 4Eu. 404 OW. 4a-u. OOM 0Inu 41u00 44 40 4M0 OM 00 0 - - 0 0 0A 0L N 0 0...UO.j 002 000 000 001- 000 U.JZ 0.1 0.1 U-JO U 0 Uhihi. 0 4xi .1 .1 ..J .1 -. - 0 ZWO EuA.W EZnW lonw Eznw Eznw Eznh Ezw EI-m E- El- E1-m E04 KMW W0...2 2I.- cc M&#34 1 4z 0 24 Ei cc 1- 1- cc cc zL u 0 4 4M U 2 I 22 c M m Lu 4 1-- E E 0 E M w Ezw a. cc 2 2 Hm < :3 U U> Lu LuI Lu E z Lu 0 Ix1 Lu z co CD

  20. GJB2 and mitochondrial A1555G gene mutations in nonsyndromic profound hearing loss and carrier frequencies in healthy individuals

    Indian Academy of Sciences (India)

    Elif Baysal; Yildirim A. Bayazit; Serdar Ceylaner; Necat Alatas; Buket Donmez; Gulay Ceylaner; Imran San; Baki Korkmaz; Akin Yilmaz; Adnan Menevse; Senay Altunyay; Bulent Gunduz; Nebil Goksu; Ahmet Arslan; Abdullah Ekmekci

    2008-04-01

    This study aimed to assess mutations in GJB2 gene (connexin 26), as well as A1555G mitochondrial mutation in both the patients with profound genetic nonsyndromic hearing loss and healthy controls. Ninety-five patients with profound hearing loss (>90 dB) and 67 healthy controls were included. All patients had genetic nonsyndromic hearing loss. Molecular analyses were performed for connexin 26 (35delG, M34T, L90P, R184P, delE120, 167delT, 235delC and IVS1+1 A → G) mutations, and for mitochondrial A1555G mutation. Twenty-two connexin 26 mutations were found in 14.7% of the patients, which were 35delG, R184P, del120E and IVS1+1 A → G. Mitochondrial A1555G mutation was not encountered. The most common GJB2 gene mutation was 35delG, which was followed by del120E, IVS1+1 A → G and R184P, and 14.3% of the patients segregated with DFNB1. In consanguineous marriages, the most common mutation was 35delG. The carrier frequency for 35delG mutation was 1.4% in the controls. 35delG and del120E populations, seems the most common connexin 26 mutations that cause genetic nonsyndromic hearing loss in this country. Nonsyndromic hearing loss mostly shows DFNB1 form of segregation.

  1. The Relationship between the Physical Activity Environment, Nature Relatedness, Anxiety, and the Psychological Well-being Benefits of Regular Exercisers

    Directory of Open Access Journals (Sweden)

    Emma Lawton

    2017-06-01

    Full Text Available Research from a variety of scientific fields suggests that physical activity in nature and feelings of connection to nature enhance psychological health and well-being. This study investigated the psychological health and well-being impact of the physical activity environment for those already undertaking the recommended weekly amount of physical activity. This topic is important for the design of health and well-being environments and interventions involving physical activity. Participants (N = 262 aged 18–71 years (M = 34.5, SD = 13.1 who met the UK physical activity guidelines completed the Nature Relatedness Scale, the trait section of the State Trait Inventory for Cognitive and Somatic Anxiety and the Psychological Well-Being Scale. Analysis via Multivariate ANOVA indicated that participants who engaged in outdoor physical activity reported significantly lower somatic anxiety levels and higher Nature Relatedness experience (NRexp. Significant results were not evident for wellbeing. Hierarchical regressions revealed that the psychological well-being facet of autonomy, NRexp, and outdoor physical activity predicted lower somatic anxiety, whereas indoor physical activity predicted higher somatic anxiety. Results indicate that somatic anxiety is lower for outdoor physical activity participation, and that outdoor activity, in conjunction with autonomy and NRexp, predicts lower anxiety levels. The findings extend previous work by demonstrating the impact of the physical activity environment on anxiety levels, as well as the contribution of outdoor physical activity and well-being facets to the previously established Nature Relatedness-anxiety relationship.

  2. Religious and Political Conservatism and Beliefs About Same-Sex Parenting in Portugal

    Directory of Open Access Journals (Sweden)

    Pedro Alexandre Costa

    2014-03-01

    Full Text Available AimDuring the last decade, there have been political changes regarding the rights of Lesbian, Gay, Bisexual, and Transgender (LGBT individuals in Portugal, such as the right to marry. However, parenting by same-sex couples is not legally allowed. The purpose of this study was to assess Portuguese heterosexuals’ beliefs about same-sex parenting, and the role of religious and political conservatism in shaping these beliefs.MethodA total of 993 participants, aged between 18 and 69 years (M = 34; SD = 11, responded to one of three questionnaires that included a case vignette depicting a different-sex, a female same-sex, or a male same-sex couple wishing to adopt a child. Participants were then asked to evaluate whether the couple would be suitable to adopt a child, and whether they anticipated any social and emotional problems with the child.ResultsParticipants consistently anticipated more children’s social and emotional problems if they were adopted by a same-sex couple. Men evaluated same-sex couples less favourably than women, and even less so the male same-sex couple.ConclusionIt was found that both religious conservatism and right-wing political leaning were associated with more sexual prejudicial beliefs regarding same-sex couples.

  3. L’occasione e l’eterno: la tenda di Tolomeo Filadelfo nei palazzi di Alessandria. Parte seconda. Una proposta di ricostruzione

    Directory of Open Access Journals (Sweden)

    Elena Calandra

    2009-05-01

    Full Text Available The work is the second part of an essay published in “Lanx” 1 (2008.After exposing the previous reconstructions, a new proposal is advanced, which explains the marquee through the macedonian architecture and suggests a new architectonical building type, the oecus aegyptius. Since only the interior is known, the dimensions are calculated (m 34 x 26 approximately for the base, m 22 for the height of the columns, with the addition of the roof, and the constitutive elements are interpreted according to the comparison with the archaeological and literary evidence: the palm- and thyrsos-shaped columns, the nymphai and the antra, the statue bases, the arch door, the lacunar ceiling, the sloping roof, the furniture, the gold and silver vases, the works of art. The exhibition lasts one year, and consequently becomes a new “event”, in connection with the contemporary dionysiac pompé: the works of art acquire a new meaning, connoting the Lagides as refined collectors, and are part of a cultural and political program, probably organized by the Museum scholars. The exhibition, indeed, is marked by an encyclopaedical purpose: on one hand it seems to be the ephemeral equivalent of the  Museum and of the zoo promoted  by Ptolemy I, on the other it takes part in the cultural construction of the Ptolemean family, being almost its dynastic monument.

  4. Community Noise Exposure Resulting from Aircraft Operations. Volume 5. Acoustic Data on Air Force Propeller Aircraft

    Science.gov (United States)

    1978-02-01

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  5. The influence of attraction to partner on heterosexual women's sexual and relationship satisfaction in long-term relationships.

    Science.gov (United States)

    Mark, Kristen P; Herbenick, Debby

    2014-04-01

    Previous research has consistently found that attraction is important in the formation of relationships though research on attraction in long-term relationships is less well understood. This article examined the predictive value of self-reported attraction to partner and change in attraction to partner on sexual and relationship satisfaction in 176 women in committed heterosexual relationships using online survey methodology. Participants' age ranged from 21 to 56 (M = 34.5) years and their relationship length ranged from 5 to 35 (M = 11.75) years. Hierarchical multiple regression results indicated that change in attraction to partner was the most salient predictor of sexual satisfaction, with current attraction to partner also related to women's sexual satisfaction, accounting for 20 % of the variance. Current attraction to partner was the only significant predictor of women's relationship satisfaction, accounting for 22 % of the variance. Additionally, attraction variables accounted for variance above and beyond the impact of relationship and sexual satisfaction. These findings suggest that self-reported attraction to partner is an important contributor to women's satisfaction outcomes in long-term relationships. Further studies in the area of attraction to partner that include couple dynamics and longitudinal data are encouraged and implications for therapists, clinicians, and educators are discussed.

  6. PTSD symptomatology and hazardous drinking as risk factors for sexual assault revictimization: examination in European American and African American women.

    Science.gov (United States)

    Littleton, Heather; Ullman, Sarah E

    2013-06-01

    A sexual victimization history is a risk factor for experiencing further sexual victimization. Posttraumatic stress disorder (PTSD) symptoms have been posited as predictors of revictimization through multiple pathways, including through their association with risk recognition and alcohol use. There is, however, limited longitudinal research examining these revictimization risk factors, including the extent to which they predict risk for forcible rape (rape involving threat or force) and incapacitated rape (rape of a victim incapacitated by substances). Additionally, there is no research evaluating ethnic differences in revictimization risk pathways. The current study examined PTSD symptoms and hazardous drinking as predictors of new forcible and incapacitated rape over 1 year in a community sample of European American (n = 217) and African American (n = 272) sexual assault victims (M = 34 years; 84% high school education or above). We hypothesized that PTSD symptoms would predict both types of revictimization and hazardous drinking would predict incapacitated rape. Results supported that PTSD symptoms predicted both types of rape (forcible rape, β = .34; incapacitated rape, β = .20), and hazardous drinking predicted incapacitated rape (β = .24). PTSD symptoms predicted hazardous drinking in African American women only (β = .20). Thus, there is a need to evaluate risk pathways for specific types of victimization among diverse samples.

  7. Repetitive negative thinking predicts depression and anxiety symptom improvement during brief cognitive behavioral therapy.

    Science.gov (United States)

    Kertz, Sarah J; Koran, Jennifer; Stevens, Kimberly T; Björgvinsson, Thröstur

    2015-05-01

    Repetitive negative thinking (RNT) is a common symptom across depression and anxiety disorders and preliminary evidence suggests that decreases in rumination and worry are related to improvement in depression and anxiety symptoms. However, despite its prevalence, relatively little is known about transdiagnostic RNT and its temporal associations with symptom improvement during treatment. The current study was designed to examine the influence of RNT on subsequent depression and anxiety symptoms during treatment. Participants (n = 131; 52% female; 93% White; M = 34.76 years) were patients presenting for treatment in a brief, cognitive behavior therapy based, partial hospitalization program. Participants completed multiple assessments of depression (Center for the Epidemiological Studies of Depression-10 scale), anxiety (the 7-item Generalized Anxiety Disorder Scale), and repetitive negative thinking (Perseverative Thinking Questionnaire) over the course of treatment. Results indicated statistically significant between and within person effects of RNT on depression and anxiety, even after controlling for the effect of time, previous symptom levels, referral source, and treatment length. RNT explained 22% of the unexplained variability in depression scores and 15% of the unexplained variability in anxiety scores beyond that explained by the control variables. RNT may be an important transdiagnostic treatment target for anxiety and depression. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. Prediction of Participation of Undergraduate University Students in a Music and Dance Master’s Degree Program

    Directory of Open Access Journals (Sweden)

    Evangelos Bebetsos

    2015-07-01

    Full Text Available The aim of the study was the investigation of students’ attitudes and intention towards their possible participation in a graduate Music and Dance Distance Learning Master’s Degree Program. The sample consisted of consisted of 229 undergraduate University students, between the ages of 20 to 63 yrs. of age (M=34.24, SD=10.70. More specifically, 134 were students of the Hellenic Open University and 95 were students of the School of Physical Education and Sport Science, of the Democritus University of Thrace. The sample completed the version the “Planned Behavior Theory” questionnaire. Results revealed differences among students of both Universities, between experienced and less experienced ones, and also among age groups. On the contrary, no sex differences in any of the questionnaire’s factors were indicated. In conclusion, the findings of this research allow a better understanding of the distance education process, which explains the attitudes and intention(s of students’ participation, and the factors that might influence theirparticular participation.

  9. Commercial Aircraft Noise Definition - L-1011 Tristar. Volume 2-L-1011-1 Data

    Science.gov (United States)

    1974-09-01

    o0 % nco E~- t-- E-4 00 E-.4 \\Io \\ L \\ n $ 0 \\ co 0 434 @10 A ~ ~ C V* C6 ~0 QOýQ2 -.- 4 2-36 5- 𔃾aCo t-~ ~~~-C 0- *D * * ** * H ~ ~ ~ ~ -b-H 0...POW. O𔃽 0𔃾Q ’U. W5-2 "Fu C6 0 O 0 0-4 -W -NNN0 44 N N N N cN 0- M-DP’J . IA -c ~ L .- ~ .. s~ w . Nl w4 m oO fL af NC Pý - 4 P-~ P. .0~ Ij~ I.. IA...0 4QP,,0 0 M.. CN ’M &#34 -" f " AA on , .. S. - C*. *e.* N P- a, aQ s 06 4 0 01 V% A Nt N @- Nif N P4 N ft p. !iMi o, -A-.0 I.’ • m l-00,,,..0 • 1

  10. An architecture study of dental plaque biofilm in vivo%原位菌斑生物膜的一种完整获得方法和结构研究

    Institute of Scientific and Technical Information of China (English)

    马瑞; 刘正; 黄正蔚; 唐子圣; 朱彩莲; 叶冬霞

    2008-01-01

    目的:通过对一种方法获得原位牙菌斑生物膜的结构分析,证明这种方法的可行性.方法:选择安装有局部可摘义齿的志愿者在基托表面粘贴塑料片的方法,在塑料片上可获得1、4.24 h牙菌斑生物膜,并用激光共聚焦扫描显微镜进行结构的观察和分析.结果:牙菌斑生物膜的细菌、基质清楚可见,1、4,24 h菌斑生物膜的平均厚度分别是(21.41±0.03)μm,(34.03±0.02)μm,(58.53±0.03)μm.结论:这种方法获取的菌斑生物膜结构完整、简便可行,是一种很好的获取原位牙菌斑生物膜的方法,可为进一步的实验提供平台.

  11. Physical Properties of Copper Based MMC Strengthened with Alumina

    Directory of Open Access Journals (Sweden)

    Kaczmar J. W.

    2014-06-01

    Full Text Available The aim of this work is the development of Cu-Al2O3 composites of copper Cu-ETP matrix composite materials reinforced by 20 and 30 vol.% Al2O3 particles and study of some chosen physical properties. Squeeze casting technique of porous compacts with liquid copper was applied at the pressure of 110 MPa. Introduction of alumina particles into copper matrix affected on the significant increase of hardness and in the case of Cu-30 vol. % of alumina particles to 128 HBW. Electrical resistivity was strongly affected by the ceramic alumina particles and addition of 20 vol. % of particles caused diminishing of electrical conductivity to 20 S/m (34.5% IACS. Thermal conductivity tests were performed applying two methods and it was ascertained that this parameter strongly depends on the ceramic particles content, diminishing it to 100 Wm-1K-1 for the composite material containing 30 vol.% of ceramic particles comparing to 400 Wm-1K-1 for the unreinforced copper. Microstructural analysis was carried out using SEM microscopy and indicates that Al2O3 particles are homogeneously distributed in the copper matrix. EDS analysis shows remains of silicon on the surface of ceramic particles after binding agent used during preparation of ceramic preforms.

  12. Environmental Assessment for Education Center Buckley Air Force Base, Colorado

    Science.gov (United States)

    2006-07-01

    Jla ’/e:J.uoISIL/ ,e:J!6%aL/:ue ’sails /e.unq ue:Juawti aA!1eN) S3:)~ nOS3 ~ l~n! In:).£L .SIMO 5U!MOJJnq Jo/pue s50p e!J!eJd o~ s~~ejJe eSJeApe le!~Ue...Od (::)9 ’eUnej ’eJOJj ’SU!e/dpOO/JfipUe/l9M) S3~~ nOS3 ~ lV’~IE>OlOI8"Z1. x ’uo!~:)nJ~Suo:) 6u!Jnp Sle!Ja~ew snopJezelj Jo aSn "(:118 󈨕SeM PI/OS ’UO...8217AI!leno) S3:>~ nOS3 ~ ~3J.’t/M &#34S x Uo!~:)nJ~Suo:) 5u!Jnp ~snp aA!~!5n.:l (":lje ’ue/c’ uo.ljejuewe/dw! ejejs ’snjejs juewu!ejje ’SUo!ss!wa) AJ.11’v’no

  13. Never smile at a crocodile: betting on electronic gaming machines is intensified by reptile-induced arousal.

    Science.gov (United States)

    Rockloff, Matthew J; Greer, Nancy

    2010-12-01

    Tourists at the Koorana Saltwater Crocodile Farm in Coowonga, Queensland, Australia, including 62 males and 41 females, aged 18-66 (M = 34.2, SD = 13.3), were randomly assigned to play a laptop-simulated Electronic Gaming Machine (EGM) either: (1) prior to entry, or (2) after having held a 1-m saltwater-crocodile. Gambling behavior; including bet-size, speed of betting, final payouts and trials played on the EGM; was investigated with respect to participants' assigned arousal condition, problem-gambling status, and affective state. At-risk gamblers with few self-reported negative emotions placed higher average bets at the EGM after having held the crocodile when compared to the control. In contrast, at-risk gamblers with many self-reported negative emotions placed lower average bets at the EGM after having held the crocodile. The results suggest that high arousal can intensify gambling in at-risk players, but only if this feeling state is not perceived as a negative emotion.

  14. Aspirin, salicylate, sulfite and tartrazine induced bronchoconstriction. Safe doses and case definition in epidemiological studies.

    Science.gov (United States)

    Corder, E H; Buckley, C E

    1995-10-01

    Allergic-like reactions to chemical components of foods and medicines may be common. The prevalence of idiosyncratic reactions to aspirin, salicylate, metabisulfite and tartrazine is not known. We used a tertiary referral clinic population to estimate safe exposure doses for epidemiological studies. A 15% decrease in the amount of air expired in one second was defined a positive response. The median effective molar doses of the agents were remarkably similar: metabisulfite 0.19 mM, 34.4 mg [95% confidence interval (CI) 0.14, 0.27 mM]; tartrazine 0.10 M, 55.0 mg (95% CI 0.05, 0.21 mM); aspirin 0.09 mM, 16.5 mg (95% CI 0.04, 0.19 mM); and salicylate 0.11 mM, 15.3 mg (95% CI 0.05, 0.27 mM). Doses to which the most sensitive (5%) and practically all (95%) susceptible persons might respectively respond are: metabisulfite 4.6 mg, 255.8 mg; tartrazine 3.4 mg, 885.6 mg; aspirin 0.8 mg, 332.3 mg; and salicylate 2.6 mg, 89.9 mg. Doses within these ranges can be used in epidemiological studies.

  15. POLYMERIZATION ,STRUCTURES AND THERMAL PROPERTIES OF PHOSPHAPHENANTHRENE-CONTAINING POLY ( 1 -PENTYNE)%以膦酰杂菲为侧基的1-戊炔聚合反应及其结构与热力学性质

    Institute of Scientific and Technical Information of China (English)

    杨帆; 佟斌; 石建兵; 支俊格; 申进波; 董宇平

    2011-01-01

    以2-(6-氧化石-氢-二苯基(c,e)<1,2>氧杂膦酰基)-1,4-二羟基苯( ODOPB)结构单元为中心,在两侧通过酯化反应分别引入4-(4-戊炔氧基)苯甲酰基和4-丁氧基苯甲酰基,得到了1-戊炔衍生物单体M-34,同时合成了不含9,10-二氢-9-氧杂-10-膦杂菲-10-氧化物(简称膦酰杂菲,DOPO)基元的M-34模型化合物M-0.分别以[Rh(nbd) C1]2和Rh(nbd) B( C6H5)4为催化剂,研究了催化剂浓度和聚合时间对M-34和M-0聚合反应收率的影响.结果表明,由于DOPO存在较大π共轭结构和较强极性效应,降低了催化剂的活性,使得聚合产物P-34分子量较低,但改善了P-34的溶解性,并诱导P-34主链基本采取全反式构型,从而有利于增强侧基之间的相互作用,使这种含有DOPO的聚1-戊炔衍生物呈现良好的热稳定性,且优于其不含DOPO模型聚合物P-0的热稳定性.但同时DOPO的引入也增加侧基的体积,为链段运动提供了较大的自由体积,使得P-34的Tg低于不含DOPO模型聚合物P-O的Tg.%The phosphaphenanthrene-containing 1-pentyne monomer ( M-34) was synthesized through the successive esterification of 2-( 6-oxide-6H-dibenz oxaphos-phorin-6-y1) -dihydroxyphenylene (ODOPB) with 4-(4-pentynoxy)benzoyl and 4-butyloxybenzoyl, respectively. Due to the introduction of 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide ( DOPO ) group, the catalytic ability of Rh-based catalysts ( [ Rh( nbd) Cl] 2 and Rh ( nbd ) B ( C6H5 ) 4 ) is decreased and the yields of polymer are relatively low. The polymerization of model monomer (M-0) in the absence of phosphaphenanthrene side-group was, however, carried out in high yield at the same condition. The microstructure of the poly ( 1-pentyne) derivants was characterized by means of 1H-NMR. The experimental results indicate that the main-chains of poly( 1-pentyne) derivants with high trans-content configuration are produced due to the strong π-π stacking and polar interactions between

  16. Non-professional marathon running: RAGE axis and ST2 family changes in relation to open-window effect, inflammation and renal function

    Science.gov (United States)

    Bekos, Christine; Zimmermann, Matthias; Unger, Lukas; Janik, Stefan; Hacker, Philipp; Mitterbauer, Andreas; Koller, Michael; Fritz, Robert; Gäbler, Christian; Kessler, Mario; Nickl, Stefanie; Didcock, Jessica; Altmann, Patrick; Haider, Thomas; Roth, Georg; Klepetko, Walter; Ankersmit, Hendrik Jan; Moser, Bernhard

    2016-01-01

    Conflicting data exist on the relevance of marathon (M) and half marathon (HM) running for health. The number of non-professional athletes finishing M and HM events is steadily growing. In order to investigate molecular changes occurring in amateur athletes, we enrolled 70 non-professional runners finishing a single M (34) or HM (36) event at baseline, the finish line and during recovery, and 30 controls. The measurement of the Receptor for Advanced Glycation Endproducts, Interleukin 1 receptor antagonist, ST2 and cytokeratin 18 was combined with molecules measured during clinical routine. Results were analyzed in the light of blood cell analysis, lactate measurements, correction for changes in plasma volume and body composition assessments. There were intrinsic differences in body mass index, abdominal body fat percentage and training time between M and HM runners. C-reactive protein changes in M and HM runners. While soluble RAGE, AGEs and ST2 increased immediately after the race in HM runners, HMGB1 increased in HM and M after the race and declined to baseline after a recovery period. We give insights into the regulation of various molecules involved in physical stress reactions and their possible implications for the cardiovascular system or renal function. PMID:27653273

  17. Solvent refined lignite process development. Quarterly technical progress report No. 3, January--March 1978

    Energy Technology Data Exchange (ETDEWEB)

    Severson, D.E.

    1978-07-12

    Six runs, each nominally at one week's duration, M-34 through M-39, were completed; this concludes the PDU experimental program for Project Lignite. The first three runs concluded the series to study the effects of different lignites on plant operability and product yields. Runs M-37 and M-38 were made using two Montana subbituminous coals. It was concluded that coals with the highest ash content, both lignite and subbituminous coals, gave greater overall conversion to gaseous and liquid organic products. The subbituminous coals gave lower gas yields and lower overall conversion while producing similar liquid yields when compared with lignite. Reactor solids build up rapidly during initial operations, then level off and remain constant at 29 pounds per cubic foot of reactor volume as a run proceeds. Reactor solids evidently are composed of clay minerals and large particles of pyridine insoluble organic material when processing either lignites or subbituminous coals; in addition calcium/magnesium carbonate crystals (calcite/dolomite) are present in the case of lignite. Long term, trouble-free operation of the deashing unit was not accomplished. Integration of the unit for several hours of combined operation of the process was achieved, and the product from the deashing unit contained as low as 0.6% ash and pyridine insoluble material. Solids buildup in the solids settling tower was a continuing problem.

  18. Tri-Service Champus Statistical Database Project (TCSDP): Champus Ambulatory Data Analysis. Detail Report

    Science.gov (United States)

    1993-03-30

    8217 > 1 IK I I I I IU I m &#34" . L1- I I M U. I 1i to I LI- -- - - -+- - - --- - 0 -- ------ -7 - - --- - TT I~ P1 .1 -I’ .1 N1 - 1 m 1 .1 * a .1 . I -wa...1tu ..i C4 a a acca I .I1 .I 4101t I- a I~ aO I - IacaI-aa. a L,. a 1u4a a -a-a ,t "I Ia -a w1 a. zuI "Ia ) i-a a a a a a I- zo ai I a I gow I l I L...d~l ~ l -w P1 P-I I l in r I U) IN 0 : (0 l 1 O 1 IC0 .1 I4M io I) ’ I I I Iý I I In I 1 I N 1 1 l I 1 0; 1~ -0 ~ 0 I I I1 1 1 M I zl I I I I I I I I

  19. 文拉法辛与呋喃唑酮治疗有害饮酒的比较%Venlafaxine vs furazolidone in treating harmful alcoholics

    Institute of Scientific and Technical Information of China (English)

    张丽; 全成实; 庞治兴; 朴贞素

    2001-01-01

    AIM: To compare the effects, adverse reactions and emotion change of venlafaxine vs furazolidone for harmful alcoholics. METHODS: Sixty-eight patients with harmful alcoholics were divided into two groups. Thirty-four patients (M34; age 37 a± s 6 a) were treated with venlafaxine, 25-50 mg, po, tid, for 8 wk. The other thirty-four patients (M34;age 38 a±6 a) with furzolidone, 0.1-0.2 g, po, tid, for 8 wk, with drinking alcohol at regular intervals during treating with furazolidone. RESULTS: The total effective rate was 79% in venlafaxine group at 3 mo. There were no obvious adverse reactions, the anxiety disorder and depressive disorder during treating with venlafaxine. And the total effective rate was 65% in furazolidone group (P<0.05). There were obvious adverse reactions such as hypotension, myocardial ischemia, anxiety disorder and depressive disorder after treating with furazolidone. CONCLUSION: Adverse reaction of venlafaxine on harmful alcoholics is smaller than furazolidone and the effect of venlafaxine on alcoholics is better than furazolidone.%目的:比较文拉法辛与呋喃唑酮对有害饮酒治疗的疗效、不良反应以及治疗过程中的心境变化。方法:有害饮酒者共68例均为男性,分为2组。文拉法辛组34例,年龄37 a± s 6 a,给文拉法辛25~50 mg,po,tid。呋喃唑酮组34例,年龄38 a±6 a,给呋喃唑酮0.1~0.2 g,po,tid,在服用呋喃唑酮期间,间断饮用白酒。结果:文拉法辛组,3 mo戒断总有效率79%,治疗过程中不良反应不明显,焦虑、抑郁分数较低。呋喃唑酮组戒断治疗总有效率65%,与文拉法辛组相比差异有显著意义(P<0.05)。治疗过程中出现血压变化、心肌缺血等药物不良反应较重,治疗后的焦虑、抑郁分数较高。结论:文拉法辛治疗有害饮酒不良反应少于呋喃唑酮,治疗有效率高于呋喃唑酮。

  20. Strategies to distinguish new synthetic cannabinoid FUBIMINA (BIM-2201) intake from its isomer THJ-2201: metabolism of FUBIMINA in human hepatocytes.

    Science.gov (United States)

    Diao, Xingxing; Scheidweiler, Karl B; Wohlfarth, Ariane; Zhu, Mingshe; Pang, Shaokun; Huestis, Marilyn A

    Since 2013, a new drugs-of-abuse trend attempts to bypass drug legislation by marketing isomers of scheduled synthetic cannabinoids (SCs), e.g., FUBIMINA (BIM-2201) and THJ-2201. It is much more challenging to confirm a specific isomer's intake and distinguish it from its structural analog because the isomers and their major metabolites usually have identical molecular weights and display the same product ions. Here, we investigated isomers FUBIMINA and THJ-2201 and propose strategies to distinguish their consumption. THJ-2201 was scheduled in the US, Japan, and Europe; however, FUBIMINA is easily available on the Internet. We previously investigated THJ-2201 metabolism in human hepatocytes, but human FUBIMINA metabolism is unknown. We aim to characterize FUBIMINA metabolism in human hepatocytes, recommend optimal metabolites to confirm its consumption, and propose strategies to distinguish between intakes of FUBIMINA and THJ-2201. FUBIMINA (10 μM) was incubated in human hepatocytes for 3 h, and metabolites were characterized with high-resolution mass spectrometry (HR-MS). We identified 35 metabolites generated by oxidative defluorination, further carboxylation, hydroxylation, dihydrodiol formation, glucuronidation, and their combinations. We recommend 5'-OH-BIM-018 (M34), BIM-018 pentanoic acid (M33), and BIM-018 pentanoic acid dihydrodiol (M7) as FUBIMINA specific metabolites. THJ-2201 produced specific metabolite markers 5'-OH-THJ-018 (F26), THJ-018 pentanoic acid (F25), and hydroxylated THJ-2201 (F13). Optimized chromatographic conditions to achieve different retention times and careful selection of specific product ion spectra enabled differentiation of isomeric metabolites, in this case FUBIMINA from THJ-2201. Our HR-MS approach should be applicable for differentiating future isomeric SCs, which is especially important when different isomers have different legal status.

  1. Prevalence and incidence of systemic sclerosis in southern Sweden: population-based data with case ascertainment using the 1980 ARA criteria and the proposed ACR-EULAR classification criteria.

    Science.gov (United States)

    Andréasson, K; Saxne, T; Bergknut, C; Hesselstrand, R; Englund, M

    2014-10-01

    To estimate the prevalence and incidence of systemic sclerosis (SSc) in southern Sweden. In Skåne, the southernmost region of Sweden (total population 1.2 million), healthcare provided is registered in the Skåne Healthcare Register. We identified all Skåne residents who had received an International Classification of Diseases 10 diagnosis of SSc (M34) or Raynaud's phenomenon (I73.0) between 1998 and 2010. Every single case was ascertained by review of medical records in reference to the 1980 American Rheumatism Association preliminary classification criteria for SSc and the proposed American College of Rheumatology (ACR)-European League Against Rheumatism (EULAR) classification criteria presented at the ACR/Association of Rheumatology Health Professionals Annual Meeting 2012. We calculated the point prevalence by the end of 2010 by linkage with the population register to exclude deceased persons and we also estimated the mean annual cumulative incidence for 2006-2010. Using the 1980 ARA criteria, the adult prevalence and annual incidence of SSc in the Skåne region were 235 and 14 per 1 million inhabitants respectively. Applying the proposed ACR-EULAR criteria, the corresponding figures were 305 and 19 per 1 million inhabitants. A majority (82%) of the prevalent cases had the limited cutaneous SSc subtype. The prevalence and incidence of SSc in southern Sweden, based on the 1980 ARA criteria, are higher than previously reported in northern Europe and do not support the concept of a north-south gradient of SSc occurrence in Europe. Application of the proposed ACR-EULAR classification criteria in this population results in about 30-40% higher estimates of SSc prevalence and incidence compared to the 1980 ARA criteria. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  2. Obesity, ultrasound indexes of fat depots and lipid goal attainment in patients with high and very high cardiovascular risk: A novel approach towards better risk reduction.

    Science.gov (United States)

    Haberka, M; Okopień, B; Gąsior, Z

    2016-02-01

    Our aim was to assess the attainment of primary (low density lipoprotein cholesterol; LDL-C) and secondary (non-high density lipoprotein cholesterol; non-HDL-C) lipid therapeutic goals in relation to obesity, clinical measures of adiposity and ultrasound indexes of fat depots, including the novel index of periarterial adipose tissue (PAT): carotid artery extra media thickness (EMT). High and very high cardiovascular (CV) risk patients (n = 420; F/M: 34/66%; age: 61.2 ± 7 years) with prior statin treatment (≥ 18 months) were enrolled into this cross-sectional study. All patients had a detailed assessment with several anthropometric measures and ultrasound indexes of fat depots indexed to BMI: abdominal (Intra-abdominal Fat Thickness; IAT and Pre-peritoneal Fat Thickness; PreFT), paracardial (Epicardial Fat Thickness; EFT and Pericardial Fat Thickness; PFT) and the new index corresponding to PAT (carotid EMT). Lipid goals attainment in the study group was as follows: 34% (LDL-C goal), 39% (non-HDL-C goal) and 35% (both LDL and non-HDL-C goals). Among ultrasound indexes, patients with both lipid goals attainment revealed significantly lower carotid EMT/BMI (LDL-C goal: 25.2 ± 4.2 vs 27.5 ± 4.1, p obesity and most clinical measures of adiposity, carotid EMT and abdominal IAT, but not other ultrasound indexes of fat depots revealed associations independent from BMI with lipid goal attainment and may help identify patients requiring more aggressive lipid management. Copyright © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved.

  3. Analisis Pengaruh Scouring Pada Pipa Bawah Laut (Studi Kasus Pipa Gas Transmisi SSWJ Jalur Pipa Gas Labuhan Maringgai – Muara Bekasi

    Directory of Open Access Journals (Sweden)

    Muhammad Catur Nugraha

    2012-09-01

    Full Text Available Abstrak— Jalur pipa gas bawah laut merupakan salah satu infrastruktur transportasi jarak jauh untuk minyak dan gas yang paling efesien untuk pemindahan produksi minyak dan gas  dibandingkan dengan penggunaan struktur terapung seperti kapal tanker. Pada struktur pipa bawah laut fenomena scouring juga dapat terjadi yang berpengaruh kepada struktur pipa. Akibat yang timbul darinya ialah adanya freespan yang dapat menyebabkan defleksi dan vibrasi atau biasa dikenal sebagai fenomena Vortex Induced Vibration (VIV. Tugas akhir ini adalah melakukan analisis pengaruh scouring pada pipa bawah laut, data yang digunakan adalah data Pipa Gas Labuhan Maringgai – Muara Bekasi yang dioperasikan oleh Perusahaan Gas Negara, berdasarkan code DnV RP F105 untuk analisa dinamis. Dari hasil analisa, maka diperoleh kedalaman scouring maksimal pada tiap zona yang dianalisa adalah 0.65 m, 0.26 m, 0.2 m, 0.15 m, 0,2 m dan 0.48 m sedankan panjang span yang dijinkan ialah panjang span yang dihitung berdasarkan gerak inline flow yaitu 29.13 m, 36.93 m, 44.53 m, 47.43 m, 34.17 m dan 30.61 m untuk zona yang sama. Dari hasil panjang span yang ijinkan tersebut didapatkan defleksi yang terjadi tiap zona yaitu  0.001873 m, 0.004013 m, 0,007476 m, 0.009259 m, 0.003117 m dan 0.003117 m. Sedangkan dari hasil analisa terjadinya osilasi pada pipa diketahui bahwa pada tiap zona menghasilkan frekuensi natural yang lebih besar daripada frekuensi vortex shedding sehingga osilasi pada pipa akibat vortex tidak terjadi dan pipa aman dioperasikan.

  4. The effect of groundwater fluctuations on the velocity pattern of slow-moving landslides

    Directory of Open Access Journals (Sweden)

    Th. W. J. van Asch

    2009-05-01

    Full Text Available Slow-moving landslides show complex mechanical and fluid interactions. They show among others non linear intrinsic viscosity of the shear zone, undrained loading effects and the generation of excess pore water pressure. The parameterization of hydrological and geomechanical factors by field and laboratory tests to describe the movement pattern of these landslides is difficult. It is a challenge to simulate accurately the de- and acceleration of these landslides and particularly, to forecast catastrophic surges.

    In this paper the relation between groundwater fluctuation and landslide velocity for two deep-seated landslides of the Trièves Plateau (the Monestier-du-Percy landslide and the Saint-Guillaume landslide is analysed. Inclinometer measurements, showing the displacement in depth after 1–2 months periods, showed on both landslides shear band deformation within 1 m. At the Monestier-du-Percy landslide, depending on the position, the shear band depths vary between 25.0 m and 10.0 m. At the Saint-Guillaume landslide, the inclinometers detected several slip surfaces inside the clays, at respectively 37.0 m, 34.5 m, and 14.0 m depth. Two simple geomechanical models are developed to describe these displacements in depth in relation to measured groundwater fluctuations. Calibration of the models using the friction angle delivered no constant values for different measuring periods. It appeared that calibrated (apparent friction values increase with increasing groundwater levels. The paper discusses the possibility of the generation of negative excess pore water pressures as a feed back mechanism, which may explain the complex displacement pattern of these landslides developed in varved clays.

  5. Yersinia enterocolitica and Photorhabdus asymbiotica β-lactamases BlaA are exported by the twin-arginine translocation pathway.

    Science.gov (United States)

    Schriefer, Eva-Maria; Hoffmann-Thoms, Stephanie; Schmid, Franz X; Schmid, Annika; Heesemann, Jürgen

    2013-01-01

    In general, β-lactamases of medically important Gram-negative bacteria are Sec-dependently translocated into the periplasm. In contrast, β-lactamases of Mycobacteria spp. (BlaC, BlaS) and the Gram-negative environmental bacteria Stenotrophomonas maltophilia (L2) and Xanthomonas campestris (Bla(XCC-1)) have been reported to be secreted by the twin-arginine translocation (Tat) system. Yersinia enterocolitica carries 2 distinct β-lactamase genes (blaA and blaB) encoding BlaA(Ye) and the AmpC-like β-lactamase BlaB, respectively. By using the software PRED-TAT for prediction and discrimination of Sec from Tat signal peptides, we identified a functional Tat signal sequence for Yersinia BlaA(Ye). The Tat-dependent translocation of BlaA(Ye) could be clearly demonstrated by using a Y. enterocolitica tatC-mutant and cell fractionation. Moreover, we could demonstrate a unique unusual temperature-dependent activity profile of BlaA(Ye) ranging from 15 to 60 °C and a high 'melting temperature' (T(M)=44.3°) in comparison to the related Sec-dependent β-lactamase TEM-1 (20-50°C, T(M)=34.9 °C). Strikingly, the blaA gene of Y. enterocolitica is present in diverse environmental Yersinia spp. and a blaA homolog gene could be identified in the closely related Photorhabdus asymbiotica (BlaA(Pa); 69% identity to BlaA(Ye)). For BlaA(Pa) of P. asymbiotica, we could also demonstrate Tat-dependent secretion. These results suggest that Yersinia BlaA-related β-lactamases may be the prototype of a large Tat-dependent β-lactamase family, which originated from environmental bacteria.

  6. Black carbon over Mexico: The effect of atmospheric transport on mixing state, mass absorption cross-section, and BC/CO ratios

    Energy Technology Data Exchange (ETDEWEB)

    Subramanian, R.; Kok, G. L.; Baumgardner, Darrel; Clarke, A. D.; Shinozuka, Y.; Campos, Teresa; Heizer, CG; Stephens, Britton; de Foy, B.; Voss, Paul B.; Zaveri, Rahul A.

    2010-01-13

    A single particle soot photometer (SP2) was operated on the NCAR C-130 during the MIRAGE campaign (part of MILAGRO), sampling black carbon (BC) over Mexico. The highest BC concentrations were measured over Mexico City (sometimes as much as 2 Fg/m34 ) and over hill fires to the south of the city. The age of plumes outside of Mexico City was determined using a combination of HYSPLIT trajectories, WRF-FLEXPART modeling and CMET balloon tracks. As expected, older, diluted air masses had lower BC concentrations. A comparison of carbon monoxide (CO) and BC suggests a CO background of around 65 ppbv, and a backgroundcorrected BC/COnet ratio of 2.89±0.89 (ng/m39 -STP)/ppbv (average ± standard deviation). This ratio is similar for fresh emissions over Mexico City, as well as for aged airmasses. Comparison of light absorption measured with a particle soot absorption photometer (PSAP) and the SP2 BC suggests a BC mass-normalized absorption cross-section (MAC) of 10.9±2.1 m212 /g at 660 nm (or 13.1 m213 /g @ 550 nm, assuming MAC is inversely dependent on wavelength). This appears independent of aging and similar to the expected absorption cross-section for aged BC, but values, particularly in fresh emissions, could be biased high due to instrument artifacts. SP2-derived BC coating indicators show a prominent thinly-coated BC mode over the Mexico City Metropolitan Area (MCMA), while older air masses show both thinly-coated and thickly-coated BC. Some 2-day-old plumes do not show a prominent thickly-coated BC mode, possibly due to preferential wet scavenging of the likely-hydrophilic thickly-coated BC.

  7. Strong earthquake activity all over the world and strong-moderate earthquake activity within and near China (December, 2000 ~ January, 2001)

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@Illustration All the data in this catalog are chosen from the 2Preliminary Seismological Report of Chine-se Seismic Stations2 (Its abbreviation is 2Monthly Report2). The catalog includes the events of M34.7 in and near China and M36 all over the world. The 2Monthly Report2 is monthly compiled by the Ninth Section of Institute of Geophysics, CSB. The origin times of earthquakes in the catalog adopt coordinated universal time (UTC) in accordance with international convention. The location of every epicenter is expressed by its latitude and longitude, at the same time by the corresponding geographical region proposed by Flinn and Engdahl. The regional names are only for references and do not imply any political significance. The number of stations used and standard deviation are also given in order to illus-trate the precision of location. The surface wave magnitude MS is measured from the records of intermediate period broad band SK seismographs and adopting the surface wave magnitude formula of Beijing Station of 1965: MS= lg(AH/T)+1.66lgD +3.5 (1°

  8. Drug dependence and psychological distress in Portuguese patients entering a substance abuse treatment center

    Directory of Open Access Journals (Sweden)

    Paulo P. P. Machado

    2007-01-01

    Full Text Available En el contexto de la situación actual de los programas de tratamiento de las toxiocodependencias, las características psicopatológicas parecen estar asociadas con resultados terapéuticos negativos. El abuso de drogas, en especial la dependencia, es una perturbación compleja que incluye síntomas cognitivos, comportamentales y fisiológicos. El amplio espectro de síntomas que caracterizan estas perturbaciones es responsable de su heterogeneidad clínica en términos de manifestación clínica, severidad, vulnerabilidad, secuelas y comorbilidad que por sí solo implica un elevado sufrimiento y necesidad de intervención en diferentes áreas. La evolución de la toxicodependencia y de la psicopatología se constituye como un gran desafío para los profesionales que trabajan en estos contextos. El presente estudio descriptivo trata de explorar algunas características principales de los individuos con problemas de toxicodependencia que iniciaron un programa de tratamiento. Participaron en el estudio 107 sujetos, con edades comprendidas entre 22 y 55 años (M = 34,84; DT = 6,44, que frecuentan un centro metropolitano de tratamiento de las toxicodependencias, 90,7% de los cuales pertenecen al sexo masculino. Los resultados señalan la presencia de algunas relaciones entre la severidad del consumo y la sintomatología psicopatológica.

  9. Strong earthquake activity all over the world and strong-moderate earthquake activity within and near China (August, 2000 ~ September, 2000)

    Institute of Scientific and Technical Information of China (English)

    陈培善

    2001-01-01

    @@Illustration All the data in this catalog are chosen from the 2Preliminary Seismological Report of Chine-se Seismic Stations2 (Its abbreviation is 2Monthly Report2). The catalog includes the events of M34.7 in and near China and M36 all over the world. The 2Monthly Report2 is monthly compiled by the Ninth Section of Institute of Geophysics, CSB. The origin times of earthquakes in the catalog adopt coordinated universal time (UTC) in accordance with international convention. The location of every epicenter is expressed by its latitude and longitude, at the same time by the corresponding geographical region proposed by Flinn and Engdahl. The regional names are only for references and do not imply any political significance. The number of stations used and standard deviation are also given in order to illus-trate the precision of location. The surface wave magnitude MS is measured from the records of intermediate period broad band SK seismographs and adopting the surface wave magnitude formula of Beijing Station of 1965: MS= lg(AH/T)+1.66lgD +3.5 (1°

  10. Microstrain-level measurement of third-order elastic constants applying dynamic acousto-elastic testing

    Science.gov (United States)

    Renaud, Guillaume; Talmant, Maryline; Marrelec, Guillaume

    2016-10-01

    The nonlinear elasticity of solids at the microstrain level has been recently studied by applying dynamic acousto-elastic testing. It is the analog of conventional quasi-static acousto-elastic experiments but the strain-dependence (or stress-dependence) of ultrasonic wave-speed is measured with an applied strain ranging from 10-7 to 10-5 and produced by a stationary elastic wave. In conventional quasi-static acousto-elastic experiments, the strain is applied in a quasi-static manner; it exceeds 10-4 and can reach 10-2. In this work, we apply dynamic acousto-elastic testing to measure the third-order elastic constants of two isotropic materials: polymethyl methacrylate and dry Berea sandstone. The peak amplitude of the dynamic applied strain is 8 × 10-6. The method is shown to be particularly suitable for materials exhibiting large elastic nonlinearity like sandstones, since the measurement is performed in the domain of validity of the third-order hyperelastic model. In contrast, conventional quasi-static acousto-elastic experiments in such materials are often performed outside the domain of validity of the third-order hyperelastic model and the stress-dependence of the ultrasonic wave-speed must be extrapolated at zero stress, leading to approximate values of the third-order elastic constants. The uncertainty of the evaluation of the third-order elastic constants is assessed by repeating multiple times the measurements and with Monte-Carlo simulations. The obtained values of the Murnaghan third-order elastic constants are l = -73 GPa ± 9%, m = -34 GPa ± 9%, and n = -61 GPa ± 10% for polymethyl methacrylate, and l = -17 000 GPa ± 20%, m = -11 000 GPa ± 10%, and n = -30 000 GPa ± 20% for dry Berea sandstone.

  11. GJB2 and GJB6 mutations in children with congenital cytomegalovirus infection.

    Science.gov (United States)

    Ross, Shannon A; Novak, Zdenek; Kumbla, Rekha A; Zhang, Kui; Fowler, Karen B; Boppana, Suresh

    2007-06-01

    Congenital cytomegalovirus (CMV) infection is a leading cause of sensorineural hearing loss (SNHL) in children. Whether connexin mutations are factors in the development of CMV-related hearing loss has not been explored. We examined gap junction protein beta-2 (GJB2) and gap junction protein beta-6 (GJB6) mutations in 149 children with congenital CMV infection and 380 uninfected neonates. Mutations in GJB2 and GJB6 were assessed by nucleotide sequencing and polymerase chain reaction (PCR) methods, respectively. The study population was predominantly African American, and 4.3% of the subjects were carriers of a connexin 26 mutation. The overall frequency of GJB2 mutations was significantly higher in the group of children with CMV infection and hearing loss (21%) compared with those with CMV infection and normal hearing (3%, p = 0.017) and the group of uninfected newborns (3.9%, p = 0.016). Eight previously reported mutations (M34T, V27I, R127H, F83L, R143W, V37I, V84L, G160S), and four novel mutations (V167M, G4D, A40T, and R160Q) were detected. None of the study children had the 342-kb deletion (delGJB6-D13S1830) in GJB6, which suggests that this mutation does not play a role in hereditary deafness in the African American population. Although GJB2 mutations were detected in children with and without CMV-related hearing loss, those with hearing loss had a higher frequency of GJB2 mutations.

  12. High Precision Photometry of Variable Stars in Clusters of Different Ages with the 1.3 m Robotically Controlled Telescope (RCT)

    Science.gov (United States)

    Guinan, E. F.; McCook, G. P.; DeWarf, L. E.; Gelderman, R. F.; McGruder, C. H.; Walter, D. K.; Howell, S. B.; Davis, D. R.; Everett, M.; Mattox, J. R.

    2003-05-01

    We discuss a new program of carrying out high precision VRI CCD photometry of several star clusters of different ages. The photometry is being carried out using the 1.3 m Robotically Controlled Telescope (RCT), located at KPNO. This program focuses on the study of variable stars in mostly open clusters. Selected astrophysically important eclipsing binaries, pulsating variables, blue stragglers, and chromospherically active variable stars will be studied. Also, searches of new variable stars will be made from the expected large samples of cluster stars. For example, photometry is planned of the several W UMa eclipsing binaries and blue straggler stars in the old open cluster NGC 188. Photometry also will be carried out of the young open cluster NGC 7790. This cluster is unique because has three confirmed classical cepheid members: CE Cas A (V ˜ +10.9 mag; F8 Ib; P = 4.446 d ), CE Cep B (V ˜ +11.0 mag; F9 Ib; 5.128 d), and CF Cas (V ˜ +11.1 mag; F8 Ib; 4.875 d). NGC 7790 also contains the 10th mag eccentric B0+B0 eclipsing binary QX Cas. The observations of QX Cas are being conducted to determine the accurate distance to this star and thus to the cluster and its cepheid members. When complete these observations, combined with spectroscopy, will permit a reliable calibration of the ``zero-point'' of the galactic cepheid Period-Luminosity Law. Another possible project is the search of light variations of PMS stars and chromospherically active stars (from star spot rotational modulations) in young clusters such as the alpha Perseus Cluster, h & χ Per, M34, and the Pleiades. Refurbishment of the RCT has been made possible by NASA grant NAG 58762. The RCT Consortium includes: Western Kentucky Univ., S. Carolina St Univ., Francis Marion Univ., Villanova Univ., and the Planetary Science Institute (PSI).

  13. Oxidative Damage and Its Possible Mechanism

    Directory of Open Access Journals (Sweden)

    Tingting Wang

    2016-06-01

    Full Text Available Purpose: The paper tries to assess the protective effect of fisetin against •OH-induced DNAdamage, then to investigate the possible mechanism.Methods: The protective effect was evaluated based on the content of malondialdehyde(MDA. The possible mechanism was analyzed using various antioxidant methods in vitro,including •OH scavenging (deoxyribose degradation, •O2- scavenging (pyrogallolautoxidation, DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays.Results: Fisetin increased dose-dependently its protective percentages against •OH-inducedDNA damage (IC50 value =1535.00±29.60 μM. It also increased its radical-scavengingpercentages in a dose-dependent manner in various antioxidants assays. Its IC50 values in•OH scavenging, •O2- scavenging, DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays, were 47.41±4.50 μM, 34.05±0.87 μM, 9.69±0.53 μM, 2.43±0.14μM, and 1.49±0.16 μM, respectively.Conclusion: Fisetin can effectively protect DNA against •OH-induced oxidative damagepossibly via reactive oxygen species (ROS scavenging approach, which is assumed to behydrogen atom (H• and/or single electron (e donation (HAT/SET pathways. In the HATpathway, the 3’,4’-dihydroxyl moiety in B ring of fisetin is thought to play an importantrole, because it can be ultimately oxidized to a stable ortho-benzoquinone form.

  14. Multiple Measures of Physical Activity, Dietary Habits and Weight Status in African American and Hispanic or Latina Women

    Science.gov (United States)

    Mama, Scherezade K.; Medina, Ashley V.; Reese-Smith, Jacqueline Y.; Banda, Jorge A.; Layne, Charles S.; Baxter, Meggin; O’Connor, Daniel P.; McNeill, Lorna; Estabrooks, Paul A.

    2015-01-01

    Compared measures of physical activity and dietary habits used in the Health Is Power (HIP) study, and described the associations of physical activity and dietary habits among African American and Hispanic or Latino women, adjusted for weight status. Cross-sectional baseline data were compared for community dwelling, healthy African American (N = 262) and Hispanic or Latina women (N = 148) who participated in HIP. Physical activity was measured using the International Physical Activity Questionnaire (IPAQ) long form, the Check And Line Questionnaire (CALQ) log and accelerometry. Dietary habits were measured using NCI 24-h recall screeners, vegetable and fruit (VF) logs and the NCI Diet History Questionnaire (DHQ). Differences in physical activity and dietary habits were assessed using simultaneous 2 (ethnicity) × 3 (weight status) ANCOVAs adjusted for age and socioeconomic status. Women (M age = 44.4 ± 10.9 years) were obese (M = 34.0 ± 9.7 kg/m2), did not meet physical activity guidelines as measured by accelerometry (M = 19.4 ± 19.1 min MVPA/day) and ate few VF (M = 2.8 ± 2.7 servings/day). DHQ variables differed by weight status. IPAQ was associated with CALQ, and CALQ with accelerometry (P < .05). IPAQ was not associated with accelerometry. Regardless of ethnicity, normal weight women did more physical activity, reported more VF consumption, and consumed more fat calories than overweight and obese women (Ps < .05). African American women did more MVPA than Hispanic or Latino women (P < .001). Relationships between behaviors and weight status suggest accelerometry and DHQ are preferable, regardless of ethnicity; and studies may capture different domains of physical activity and dietary habits depending on measure used. PMID:21519867

  15. Recognizing primary emotions in communication in adults with intellectual disability

    Directory of Open Access Journals (Sweden)

    Đorđević Mirjana

    2017-01-01

    Full Text Available The ability to recognize emotions helps people understand social situations and plan their own behavior in specific social contexts. Paralinguistic segments of communication (facial expression and prosodic characteristics significantly contribute to drawing conclusions on the speaker's emotions, and according to some authors, they are considered even more dominant and more reliable than linguistic segments in decoding emotions tasks. The aim of this paper was to determine the ability to recognize primary emotions in communication with the help of paralinguistic indicators, in adults with mild and moderate intellectual disability. The sample consisted of 60 adult participants with mild (N=25 and moderate (N=35 intellectual disability, equal with regard to gender and type of housing. The participants with mild intellectual disability were aged 20-55 (M=31.24; SD=8.84, while those with moderate intellectual disability were 22-55 years of age (M=34.17; SD=8.76. The Assessment Battery for Communication, ABaCo, (Sacco et al., 2008 was used for assessing the ability to understand emotions in communication. The obtained results showed that adults with intellectual disability experienced most difficulties in detecting fear. By applying the T test for independent samples, it was determined that there were no statistically significant differences between men and women both with mild and moderate ID with regard to their ability to recognize emotions (p>0,05. With regard to the type of housing, statistically significant differences were obtained only in the group of participants with moderate intellectual disability in detecting fear (t (33=-3.51; p=0.01, in favor of those who lived in institutions. The length of living in an institution positively and moderately correlated with the ability to recognize anger in adults with intellectual disability (r=0.46, p<0.05.

  16. A comparative study of the effect of icodextrin based peritoneal dialysis and hemodialysis on lipid metabolism.

    Science.gov (United States)

    Kadiroğlu, A K; Ustündag, S; Kayabaşi, H; Yilmaz, Z; Yildirım, Y; Sen, S; Yilmaz, M E

    2013-09-01

    Dyslipidemia is frequent in patients with end stage renal disease. Excessive peritoneal glucose absorption from high glucose-containing peritoneal dialysis solutions may enhance disturbances on the lipid metabolism of patients on peritoneal dialysis. We compared the effect of icodextrin-based peritoneal dialysis therapy with hemodialysis (HD) therapy on lipid metabolism. A total of 157 non-diabetic patients on dialysis at least for 3 months; 78 patients on Icodextrin-based continuous ambulatory peritoneal dialysis (CAPD) (44 M, 34 F) and 79 patients in HD group (47M, 32F) were included into the study. After 12 h of fasting and before the dialysis session, serum urea, creatinin, glucose, Sodium, potasium, and albumin, total cholesterol (TC), triglycerides (TG), very low density lipoprotein (VLDL), low density lipoprotein (LDL)-C, high-density lipoprotein (HDL)-C, apolipoprotein A (Apo A), apolipoprotein B, and lipoprotein a were measured. TG (P = 0018) and VLDL (P = 0.022) were lower in CAPD group than HD group, HDL-C (P < 0.001) and Apo A (P = 0.001) were higher in CAPD group than in HD group. A total of 24.4% in CAPD group and 11.4% in HD group (P < 0.034) had normal serum levels of TG, LDL-C, and HDL-C. More patients in CAPD group (47.4%) had high serum Apo A levels than in HD group (21.5%) (P = 0.001). We suggest that patients receiving icodextrin-based CAPD may have better TG, HDL-C, and Apo A levels than patients on HD.

  17. Fisetin Protects DNA Against Oxidative Damage and Its Possible Mechanism

    Science.gov (United States)

    Wang, Tingting; Lin, Huajuan; Tu, Qian; Liu, Jingjing; Li, Xican

    2016-01-01

    Purpose: The paper tries to assess the protective effect of fisetin against •OH-induced DNA damage, then to investigate the possible mechanism. Methods: The protective effect was evaluated based on the content of malondialdehyde (MDA). The possible mechanism was analyzed using various antioxidant methods in vitro, including •OH scavenging (deoxyribose degradation), •O2- scavenging (pyrogallol autoxidation), DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays. Results: Fisetin increased dose-dependently its protective percentages against •OH-induced DNA damage (IC50 value =1535.00±29.60 µM). It also increased its radical-scavenging percentages in a dose-dependent manner in various antioxidants assays. Its IC50 values in •OH scavenging, •O2- scavenging, DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays, were 47.41±4.50 µM, 34.05±0.87 µM, 9.69±0.53 µM, 2.43±0.14 µM, and 1.49±0.16 µM, respectively. Conclusion: Fisetin can effectively protect DNA against •OH-induced oxidative damage possibly via reactive oxygen species (ROS) scavenging approach, which is assumed to be hydrogen atom (H•) and/or single electron (e) donation (HAT/SET) pathways. In the HAT pathway, the 3’,4’-dihydroxyl moiety in B ring of fisetin is thought to play an important role, because it can be ultimately oxidized to a stable ortho-benzoquinone form. PMID:27478791

  18. The S1(n,π*) states of cyclopentanone and cyclobutanone in a supersonic nozzle beam

    Science.gov (United States)

    Baba, Masaaki; Hanazaki, Ichiro

    1984-12-01

    Fluorescence excitation spectra of cyclopentanone and cyclobutanone have been observed for their (n,π*) transition in a pulsed supersonic nozzle beam using a high power tunable laser. A drastic reduction of hot bands has been attained, making it possible to discuss the vibronic assignments in more detail than the previous works. The C=O out-of-plane wagging mode was found to be active for both molecules. The ring twisting and flapping in cyclopentanone and the ring puckering in cyclobutanone were also active. The molecules are pyramidally distorted in the excited states with double minimum potentials in the out-of-plane displacement coordinates. The barrier to inversion (V) and the C=O out-of-plane angle at the potential minimum (θm) have been determined for the S1(n,π*) state; V=680±17 cm-1 and θm=34° for cyclopentanone, and V=1850±50 cm-1 and θm=42° for cyclobutanone. The puckering mode (ν20') of cyclobutanone was also found to have a double minimum potential with V=16.9 cm-1. The rotational envelope of each vibronic band has been analyzed on the basis of a computer simulation for an asymmetric top molecule. In contrast with formaldehyde, the A-type (parallel) component, as well as the B-type, was shown to be important in these cyclic ketones. The mechanism of the vibronic intensity borrowing is discussed on the basis of the band-type considerations.

  19. The neurochemistry and social flow of singing: bonding and oxytocin.

    Science.gov (United States)

    Keeler, Jason R; Roth, Edward A; Neuser, Brittany L; Spitsbergen, John M; Waters, Daniel J M; Vianney, John-Mary

    2015-01-01

    Music is used in healthcare to promote physical and psychological well-being. As clinical applications of music continue to expand, there is a growing need to understand the biological mechanisms by which music influences health. Here we explore the neurochemistry and social flow of group singing. Four participants from a vocal jazz ensemble were conveniently sampled to sing together in two separate performances: pre-composed and improvised. Concentrations of plasma oxytocin and adrenocorticotropic hormone (ACTH) were measured before and after each singing condition to assess levels of social affiliation, engagement and arousal. A validated assessment of flow state was administered after each singing condition to assess participants' absorption in the task. The feasibility of the research methods were assessed and initial neurochemical data was generated on group singing. Mean scores of the flow state scale indicated that participants experienced flow in both the pre-composed (M = 37.06) and improvised singing conditions (M = 34.25), with no significant difference between conditions. ACTH concentrations decreased in both conditions, significantly so in the pre-composed singing condition, which may have contributed to the social flow experience. Mean plasma oxytocin levels increased only in response to improvised singing, with no significant difference between improvised and pre-composed singing conditions observed. The results indicate that group singing reduces stress and arousal, as measured by ACTH, and induces social flow in participants. The effects of pre-composed and improvised group singing on oxytocin are less clear. Higher levels of plasma oxytocin in the improvised condition may perhaps be attributed to the social effects of improvising musically with others. Further research with a larger sample size is warranted.

  20. The Neurochemistry and Social Flow of Singing: Bonding and Oxytocin

    Directory of Open Access Journals (Sweden)

    Jason R. Keeler

    2015-09-01

    Full Text Available Music is used in healthcare to promote physical and psychological well-being. As clinical applications of music continue to expand, there is a growing need to understand the biological mechanisms by which music influences health. Here we explore the neurochemistry and social flow of group singing. 4 participants from a vocal jazz ensemble were conveniently sampled to sing together in two separate performances: pre-composed and improvised. Concentrations of plasma oxytocin and adrenocorticotropic hormone (ACTH were measured before and after each singing condition to assess levels of social affiliation, engagement and arousal. A validated assessment of flow state was administered after each singing condition to assess participants’ absorption in the task. The feasibility of the research methods were assessed and initial neurochemical data was generated on group singing. Mean scores of the flow state scale indicated that participants experienced flow in both the pre-composed (M=37.06 and improvised singing conditions (M=34.25, with no significant difference between conditions. ACTH concentrations decreased in both conditions, significantly so in the pre-composed singing condition, which may have contributed to the social flow experience. Mean plasma oxytocin levels increased only in response to improvised singing, with no significant difference between improvised and pre-composed singing conditions observed. The results indicate that group singing reduces stress and arousal, as measured by ACTH, and induces social flow in participants. The effects of pre-composed and improvised group singing on oxytocin are less clear. Higher levels of plasma oxytocin in the improvised condition may perhaps be attributed to the social effects of improvising musically with others. Further research with a larger sample size is warranted.

  1. Assessment of Maximum Aerobic Capacity and Anaerobic Threshold of Elite Ballet Dancers.

    Science.gov (United States)

    Wyon, Matthew A; Allen, Nick; Cloak, Ross; Beck, Sarah; Davies, Paul; Clarke, Frances

    2016-09-01

    An athlete's cardiorespiratory profile, maximal aerobic capacity, and anaerobic threshold is affected by training regimen and competition demands. The present study aimed to ascertain whether there are company rank differences in maximal aerobic capacity and anaerobic threshold in elite classical ballet dancers. Seventy-four volunteers (M 34, F 40) were recruited from two full-time professional classical ballet companies. All participants completed a continuous incremental treadmill protocol with a 1-km/hr speed increase at the end of each 1-min stage until termination criteria had been achieved (e.g., voluntary cessation, respiratory exchange ratio <1.15, HR ±5 bpm of estimated HRmax). Peak VO2 (5-breathe smooth) was recorded and anaerobic threshold calculated using ventilatory curve and ventilatory equivalents methods. Statistical analysis reported between-subject effects for gender (F1,67=35.18, p<0.001) and rank (F1,67=8.67, p<0.001); post hoc tests reported soloists (39.5±5.15 mL/kg/min) as having significantly lower VO2 peak than artists (45.9±5.75 mL/kg/min, p<0.001) and principal dancers (48.07±3.24 mL/kg/min, p<0.001). Significant differences in anaerobic threshold were reported for age (F1,67=7.68, p=0.008) and rank (F1,67=3.56, p=0.034); post hoc tests reported artists (75.8±5.45%) having significantly lower anaerobic threshold than soloists (80.9±5.71, p<0.01) and principals (84.1±4.84%, p<0.001). The observed differences in VO2 peak and anaerobic threshold between the ranks in ballet companies are probably due to the different rehearsal and performance demands.

  2. Molecular epidemiology of DFNB1 deafness in France

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    Molinari Nicolas

    2004-03-01

    Full Text Available Abstract Background Mutations in the GJB2 gene have been established as a major cause of inherited non syndromic deafness in different populations. A high number of sequence variations have been described in the GJB2 gene and the associated pathogenic effects are not always clearly established. The prevalence of a number of mutations is known to be population specific, and therefore population specific testing should be a prerequisite step when molecular diagnosis is offered. Moreover, population studies are needed to determine the contribution of GJB2 variants to deafness. We present our findings from the molecular diagnostic screening of the GJB2 and GJB6 genes over a three year period, together with a population-based study of GJB2 variants. Methods and results Molecular studies were performed using denaturing High Performance Liquid Chromatograghy (DHPLC and sequencing of the GJB2 gene. Over the last 3 years we have studied 159 families presenting sensorineural hearing loss, including 84 with non syndromic, stable, bilateral deafness. Thirty families were genotyped with causative mutations. In parallel, we have performed a molecular epidemiology study on more than 3000 dried blood spots and established the frequency of the GJB2 variants in our population. Finally, we have compared the prevalence of the variants in the hearing impaired population with the general population. Conclusion Although a high heterogeneity of sequence variation was observed in patients and controls, the 35delG mutation remains the most common pathogenic mutation in our population. Genetic counseling is dependent on the knowledge of the pathogenicity of the mutations and remains difficult in a number of cases. By comparing the sequence variations observed in hearing impaired patients with those sequence variants observed in general population, from the same ethnic background, we show that the M34T, V37I and R127H variants can not be responsible for profound or severe

  3. A comparative study of the effect of icodextrin based peritoneal dialysis and hemodialysis on lipid metabolism

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    A K Kadiroglu

    2013-01-01

    Full Text Available Dyslipidemia is frequent in patients with end stage renal disease. Excessive peritoneal glucose absorption from high glucose-containing peritoneal dialysis solutions may enhance disturbances on the lipid metabolism of patients on peritoneal dialysis. We compared the effect of icodextrin-based peritoneal dialysis therapy with hemodialysis (HD therapy on lipid metabolism. A total of 157 non-diabetic patients on dialysis at least for 3 months; 78 patients on Icodextrin-based continuous ambulatory peritoneal dialysis (CAPD (44 M, 34 F and 79 patients in HD group (47M, 32F were included into the study. After 12 h of fasting and before the dialysis session, serum urea, creatinin, glucose, Sodium, potasium, and albumin, total cholesterol (TC, triglycerides (TG, very low density lipoprotein (VLDL, low density lipoprotein (LDL-C, high-density lipoprotein (HDL-C, apolipoprotein A (Apo A, apolipoprotein B, and lipoprotein a were measured. TG ( P = 0018 and VLDL ( P = 0.022 were lower in CAPD group than HD group, HDL-C ( P < 0.001 and Apo A ( P = 0.001 were higher in CAPD group than in HD group. A total of 24.4% in CAPD group and 11.4% in HD group ( P < 0.034 had normal serum levels of TG, LDL-C, and HDL-C. More patients in CAPD group (47.4% had high serum Apo A levels than in HD group (21.5% ( P = 0.001. We suggest that patients receiving icodextrin-based CAPD may have better TG, HDL-C, and Apo A levels than patients on HD.

  4. Martian upper atmospheric aerosol properties from Phobos eclipse observation

    Science.gov (United States)

    Lemmon, Mark T.

    2015-11-01

    Solar occultation photometry is a useful method for probing upper atmospheric aerosols, using a long atmospheric path for direct extinction measurements. During April-June 2015, the Mars Science Laboratory’s Mastcam was used for solar occultation photometry by proxy: 3 eclipse ingresses by Phobos into Mars’ shadow were observed, as were 3 egresses from the shadow. The observations occurred in late Southern summer, at LS 331-352°. The observations of the moon’s brightness sample the Martian atmosphere along the lines of site from the Sun to Phobos. The ingresses and egresses sampled longitudes up to 1000s of km west or east of the rover’s position, respectively; sampled latitudes from 30° S to 7° S over time; and sampled local sunset or sunrise, respectively. Each eclipse was imaged with both Mastcam cameras, M-100 with an RGB filter (638, 551, and 493 nm) and M-34 with an 867-nm filter. Light-curves for the eclipses were derived from the images and interpreted via a geometric model of the event, accounting for the full range of lines of sight through the atmosphere. The altitude of 50% extinction was found to vary within the 40-60 km range. Extinction varied with wavelength: four events showed significantly higher extinction in the blue, with a monotonic decrease with wavelength, interpreted as a result of 0.3-0.4 μm dust aerosols. Two events (one of each type) showed no significant wavelength variation of extinction, interpreted as a result of large (>1 μm) aerosols. One of these, probing local sunrise conditions, may suggest a thin layer of CO2 ice cloud. Future work may allow retrieval of vertical gradients in aerosol size near the mid-point of the sensitive region (i.e., altitudes near that of 50% transmission and/or path optical depth unity) and/or identification of discrete layers vs. well-mixed aerosols (for instance, clouds vs. dust)

  5. In vitro drug metabolism of green tea catechins in human, monkey, dog, rat and mouse hepatocytes.

    Science.gov (United States)

    Chen, Wendy W; Qin, Geng-Yao; Zhang, Ting; Feng, Wan-Yong

    2012-06-01

    The metabolic fate of green tea catechins [(-)-epicatechin ((-)-EC), (-)-epicatechin-3-gallate (ECG) (-)- epigallocatechin (EGC) and (-)-epigallocatechin-3-gallate (EGCG)] in cryopreserved human, monkey, dog, rat and mouse hepatocytes was studied. Methylation, glucuronidation, sulfation and isomerization pathways of (-)-EC in all five species were found. Methylation, glucuronidation, sulfation, hydrolysis, isomerization and glucosidation pathways of ECG were found. Species differences in metabolism of (-)-EC or ECG were observed. Surprisingly, no metabolites of EGC or EGCG were detected, but chemical oxidation and polymerization were observed under these experimental conditions. It appeared that enzymatic reactions and chemical reactions were differentiated by an additional hydroxyl group on the B-ring between (-)-EC/ECG and EGC/EGCG. For (-)-EC, thirty-five metabolites including isomerized (M6. M10 and M25), glucuronidated (M3, M5 and M11), sulfated (M7, M15, M16, M18, M20, M23, M26), methylated (M2, M9, M12, M17, M19, M21, M27, M30, M32), glucuronated/methylated (M4, M8, M13, M14), sulfated/methylated (M22, M24, M28, M29, M31, M33, M34, M35) and diglucuronidate (M1), were detected and characterized. M11, M18, M19 and M23 were major metabolites in human hepatocytes; M11, M26 and M31 were major metabolites in monkey hepatocytes; M10, M20, M22, M26 and M31 were major metabolites in dog hepatocytes; M5, M6 and M10 were major metabolites in rat hepatocytes; and M5, M6 and M13 were major metabolites in mouse hepatocytes. For ECG, twelve metabolites including isomerized (M1), hydrolyzed (M3), glucosidated (M2), glucuronidated (M4 and M6), sulfated (M9, M11 and M12), methylated (M7), sulfated/glucuronidated/methylated (M8 and M10) and diglucuronidated (M5), were detected and characterized. M4, M11 and M12 were major metabolites in human hepatocytes; M11 and M12 were major metabolites in monkey hepatocytes; M3 and M11 were major metabolites in dog hepatocytes; M4, M6 and

  6. Interaction between Nbp35 and Cfd1 proteins of cytosolic Fe-S cluster assembly reveals a stable complex formation in Entamoeba histolytica.

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    Shadab Anwar

    Full Text Available Iron-Sulfur (Fe-S proteins are involved in many biological functions such as electron transport, photosynthesis, regulation of gene expression and enzymatic activities. Biosynthesis and transfer of Fe-S clusters depend on Fe-S clusters assembly processes such as ISC, SUF, NIF, and CIA systems. Unlike other eukaryotes which possess ISC and CIA systems, amitochondriate Entamoeba histolytica has retained NIF & CIA systems for Fe-S cluster assembly in the cytosol. In the present study, we have elucidated interaction between two proteins of E. histolytica CIA system, Cytosolic Fe-S cluster deficient 1 (Cfd1 protein and Nucleotide binding protein 35 (Nbp35. In-silico analysis showed that structural regions ranging from amino acid residues (P33-K35, G131-V135 and I147-E151 of Nbp35 and (G5-V6, M34-D39 and G46-A52 of Cfd1 are involved in the formation of protein-protein complex. Furthermore, Molecular dynamic (MD simulations study suggested that hydrophobic forces surpass over hydrophilic forces between Nbp35 and Cfd1 and Van-der-Waal interaction plays crucial role in the formation of stable complex. Both proteins were separately cloned, expressed as recombinant fusion proteins in E. coli and purified to homogeneity by affinity column chromatography. Physical interaction between Nbp35 and Cfd1 proteins was confirmed in vitro by co-purification of recombinant Nbp35 with thrombin digested Cfd1 and in vivo by pull down assay and immunoprecipitation. The insilico, in vitro as well as in vivo results prove a stable interaction between these two proteins, supporting the possibility of its involvement in Fe-S cluster transfer to target apo-proteins through CIA machinery in E. histolytica. Our study indicates that initial synthesis of a Fe-S precursor in mitochondria is not necessary for the formation of Cfd1-Nbp35 complex. Thus, Cfd1 and Nbp35 with the help of cytosolic NifS and NifU proteins can participate in the maturation of non-mitosomal Fe-S proteins

  7. Pyrophaeophorbide- a as a tracer of suspended particulate organic matter from the NE Pacific continental margin

    Science.gov (United States)

    Bianchi, Thomas S.; Bauer, James E.; Druffel, Ellen R. M.; Lambert, Corey D.

    Pyrophaeophorbide- a, a degradation product of chlorophyll- a, is predominantly formed by grazing processes in sediments as well as in the water column. Water column profiles of pyrophaeophorbide- a/suspended particulate organic carbon (SPOC) concentrations, at an abyssal site in the northeast (NE) Pacific (Sta M, 34°50'N, 123°00'W; 4100 m water depth), show low concentrations (0.01-0.1 ng/μg SPOC) at surface and mesopelagic depths, and increasing concentrations with closer proximity to the sea floor (0.05-0.6 ng/μg SPOC). However, in June 1992, the deep maximum of pyrophaeophorbide- a/SPOC in the water column of Sta M extended higher into the water column, as much as 1600 m above the bottom (mab) (2500 m water depth); in other seasons they only extended up to 650 mab (3450 m water depth). Previous studies have demonstrated lateral transport of particulate matter from the continental shelf to the deep ocean off the coast of northern California. Recent work suggests that the benthic boundary layer (BBL) extends to 50 mab, based on sediment trap and transmissometry measurements (Smith, K.L., Kaukmann, R.S., Baldwin, R.J., 1994. Coupling of near-bottom pelagic and benthic processes at abyssal depths. Limnology and Oceanography 39, 1101-1118.), and that lateral transport is significant only during summer, which is consistent with our observations. A partial vertical profile of pyrophaeophorbide- a/SPOC from the north central (NC) Pacific provides some evidence that the deep maximum may be absent due to the distance of this site from the continental margin. Thus, the observed deep maximum of pyrophaeophorbide- a/SPOC at Sta M is likely due mainly to lateral transport from the continental slope rather than to local vertical resuspension in the BBL exclusively. Pyrophaeophorbide- a concentrations in SPOC at Sta M were negatively correlated with Δ 14C values of SPOC (SPOC samples from Druffel, E.R.M., Bauer, J.E., Williams, P.M., Griffin, S.A. and Wolgast, D., 1996

  8. Suppression of nitric oxide production in mouse macrophages by soybean flavonoids accumulated in response to nitroprusside and fungal elicitation

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    Tamashiro Wirla MSC

    2004-04-01

    Full Text Available Abstract Background The anti-inflammatory properties of some flavonoids have been attributed to their ability to inhibit the production of NO by activated macrophages. Soybean cotyledons accumulate certain flavonoids following elicitation with an extract of the fungal pathogen Diaporthe phaseolorum f. sp. meridionalis (Dpm. Sodium nitroprusside (SNP, a nitric oxide donor, can substitute for Dpm in inducing flavonoid production. In this study, we investigated the effect of flavonoid-containing diffusates obtained from Dpm- and SNP-elicited soybean cotyledons on NO production by lipopolysaccharide (LPS- and LPS plus interferon-γ (IFNγ-activated murine macrophages. Results Significant inhibition of NO production, measured as nitrite formation, was observed when macrophages were activated in the presence of soybean diffusates from Dpm- or SNP-elicited cotyledons. This inhibition was dependent on the duration of exposure to the elicitor. Daidzein, genistein, luteolin and apigenin, the main flavonoids present in diffusates of elicited cotyledons, suppressed the NO production by LPS + IFNγ activated macrophages in a concentration-dependent manner, with IC50 values of 81.4 μM, 34.5 μM, 38.6 μM and 10.4 μM respectively. For macrophages activated with LPS alone, the IC50 values were 40.0 μM, 16.6 μM, 10.4 μM and 2.8 μM, respectively. Western blot analysis showed that iNOS expression was not affected by daidzein, was reduced by genistein, and was abolished by apigenin, luteolin and Dpm- and SNP-soybean diffusates at concentrations that significantly inhibited NO production by activated macrophages. Conclusions These results suggest that the suppressive effect of flavonoids on iNOS expression could account for the potent inhibitory effect of Dpm- and SNP-diffusates on NO production by activated macrophages. Since the physiological concentration of flavonoids in plants is normally low, the treatment of soybean tissues with SNP may provide a simple

  9. Terrestrial research in Mars analogue environments

    Science.gov (United States)

    Osipov, G.

    Fatty acids (FA) content was measured by GC-MS SIM technique in Sulfide ores of present day (Mid-Atlantic Ridge and others) and ancient (Ural Paleocene, Russia) black smokers; Early Proterozoic kerites of Volyn; Siberian, Canadian and Antarctic permafrosts and also in rocks of East-European platform Achaean crystalline basement. Analysis was shown presence those and only those fatty acids which are specific to microorganisms. FA with 12 up 19 of carbon atoms are thought to be a bacterial biomass sign. 3-Hydroxy fatty acids also found in samples and are strong specific markers of gram-negative bacteria. Cultivation yield living bacteria in some cases. The East-European platform Achaean crystalline basement rocks opened by Vorotilov Deep Well (VDW) drilled through Puchezh-Katunski impact structure were studied within depths 2575 - 2805 m. 34 microbial lipid markers were detected by GC-MS and 22 species were identified. Bacteria of g. Bacillus reached 6,8 % in subsurface communities. However, members of gg. Clostridium (37,1 - 33,2 %) and Rhodococcus (27,6 - 33,7 %) were absolute dominants within studied depth interval. Some lipid patterns of kerite samples could be assessed to definite genera or, in special cases, to species of contemporary microorganisms. For instance, 2-hydroxylauric acid is specific to Pseudomonas putida group or Acinetobacter spp., and hydroxymyristic together with hydroxypalmitic are specific to P.cepacea and cyanobacteria. 3-hydroxystearic acid was known as component of Acetobacter diazothrophycus and Gloebacter violaceous cyanobacterium. 10-hydroxystearic acid associated with Nocardia spp., which oxidizes oleic acid in organic substrates. 10-methylhexadecanoic (10Me16) acid together with 10Me14, 10Me15 and 10Me17 analogues are markers of actinomycetes. Significant part of Black Smokers organic matter is probably biogenic. Fatty acid features strongly assigns it to bacterial, microeucariotic and planta cells. Par example 3-hydroxy acids are

  10. Biomonitoring of essential and toxic metals in single hair using on-line solution-based calibration in laser ablation inductively coupled plasma mass spectrometry.

    Science.gov (United States)

    Dressler, Valderi L; Pozebon, Dirce; Mesko, Marcia Foster; Matusch, Andreas; Kumtabtim, Usarat; Wu, B; Sabine Becker, J

    2010-10-15

    Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful and sensitive surface analytical technique for the determination of concentration and distribution of trace metals within biological systems at micrometer spatial resolution. LA-ICP-MS allows easy quantification procedures if suitable standard references materials (SRM) are available. In this work a new SRM-free approach of solution-based calibration method in LA-ICP-MS for element quantification in hair is described. A dual argon flow of the carrier gas and nebulizer gas is used. A dry aerosol produced by laser ablation (LA) of biological sample and a desolvated aerosol generated by pneumatic nebulization (PN) of standard solutions are carried by two different flows of argon as carrier or nebulizer gas, respectively and introduced separately in the injector tube of a special ICP torch, through two separated apertures. Both argon flows are mixed directly in the ICP torch. External calibration via defined standard solutions before analysis of single hair was employed as calibration strategy. A correction factor, calculated using hair with known analyte concentration (measured by ICP-MS), is applied to correct the different elemental sensitivities of ICP-MS and LA-ICP-MS. Calibration curves are obtained by plotting the ratio of analyte ion M(+)/(34)S(+) ion intensities measured using LA-ICP-MS in dependence of analyte concentration in calibration solutions. Matrix-matched on-line calibration in LA-ICP-MS is carried out by ablating of human hair strands (mounted on a sticky tape in the LA chamber) using a focused laser beam in parallel with conventional nebulization of calibration solutions. Calibrations curves of Li, Na, Mg, Al, K, V, Cr, Mn, Fe, Ni, Co, Cu, Zn, Sr, Mo, Ag, Cd, I, Hg, Pb, Tl, Bi and U are presented. The linear correlation coefficients (R) of calibration curves for analytes were typically between 0.97 and 0.999. The limits of detection (LODs) of

  11. Dihydrogen phosphate-water tape and layers vs dihydrogen phosphate layers tuned by hydrophobic isomeric pyridine-diamine functionalized molecules

    Science.gov (United States)

    Huang, Jing; Liu, Tong-Peng; Huo, Li-Hua; Deng, Zhao-Peng; Gao, Shan

    2017-01-01

    Assembly of six isomeric pyridine-diamine-based molecules, N,N‧-bis(pyridin-4-ylmethyl)ethane-1,2-diamine (M1), N,N‧-bis(pyridin-3-ylmethyl)ethane-1,2-diamine (M2), N,N‧-bis(pyridin-2-ylmethyl)ethane-1,2-diamine (M3), N,N‧-bis(pyridin-4-ylmethyl)propane-1,3-diamine (M4), N,N‧-bis(pyridin-3-ylmethyl)propane-1,2-diamine (M5), and N,N‧-bis(pyridin-2-ylmethyl)propane-1,3-diamine (M6), with phosphoric acid (H3PO4) in different ratio (1:2 and 1:4), leads to the formation of nine salts, H2M12+·2H2PO4-·4H2O (1), H2M22+·2H2PO4-·2H2O (2), H2M32+·2H2PO4-·2H2O (3), H4M14+·4H2PO4- (4), H4M24+·4H2PO4- (5), H4M34+·4H2PO4- (6), H2M42+·2H2PO4-·3H2O (7), 2H2M52+·4H2PO4-·2H3PO4 (8), and H2M62+·2H2PO4- (9), which have been characterized by elemental analysis, IR, TG, PL, powder and single-crystal X-ray diffraction. Structural analyses indicate that hydrogen-bonding patterns of H2PO4- anions, conformation of protonated cations can effectively influence the supramolecular architectures through diverse non-covalent interactions. Hydrous salts 1-3 and 7 present 2D and 3D host-guest supramolecular networks, in which the connection of H2PO4- anions and water molecules generates diverse tape and layer motifs. H2PO4- anions in anhydrous salts 4-6 interconnect with each other through hydrogen bonds to form two types of layers, which are joined by discrete H4M4+ cations into 3D inorganic-organic hybrid supramolecular networks. Salts 8-9 also present 2D and 3D host-guest supramolecular networks where the interconnection of H2PO4- anions and its combination with H3PO4 molecules leads to diverse layers. Luminescent analyses indicate that salts 1-9 exhibit violet and blue emission maximum in the range of 390-467 nm at room temperature.

  12. Interaction between Nbp35 and Cfd1 proteins of cytosolic Fe-S cluster assembly reveals a stable complex formation in Entamoeba histolytica.

    Science.gov (United States)

    Anwar, Shadab; Dikhit, Manas Ranjan; Singh, Krishn Pratap; Kar, Rajiv Kumar; Zaidi, Amir; Sahoo, Ganesh Chandra; Roy, Awadh Kishore; Nozaki, Tomoyoshi; Das, Pradeep; Ali, Vahab

    2014-01-01

    Iron-Sulfur (Fe-S) proteins are involved in many biological functions such as electron transport, photosynthesis, regulation of gene expression and enzymatic activities. Biosynthesis and transfer of Fe-S clusters depend on Fe-S clusters assembly processes such as ISC, SUF, NIF, and CIA systems. Unlike other eukaryotes which possess ISC and CIA systems, amitochondriate Entamoeba histolytica has retained NIF & CIA systems for Fe-S cluster assembly in the cytosol. In the present study, we have elucidated interaction between two proteins of E. histolytica CIA system, Cytosolic Fe-S cluster deficient 1 (Cfd1) protein and Nucleotide binding protein 35 (Nbp35). In-silico analysis showed that structural regions ranging from amino acid residues (P33-K35, G131-V135 and I147-E151) of Nbp35 and (G5-V6, M34-D39 and G46-A52) of Cfd1 are involved in the formation of protein-protein complex. Furthermore, Molecular dynamic (MD) simulations study suggested that hydrophobic forces surpass over hydrophilic forces between Nbp35 and Cfd1 and Van-der-Waal interaction plays crucial role in the formation of stable complex. Both proteins were separately cloned, expressed as recombinant fusion proteins in E. coli and purified to homogeneity by affinity column chromatography. Physical interaction between Nbp35 and Cfd1 proteins was confirmed in vitro by co-purification of recombinant Nbp35 with thrombin digested Cfd1 and in vivo by pull down assay and immunoprecipitation. The insilico, in vitro as well as in vivo results prove a stable interaction between these two proteins, supporting the possibility of its involvement in Fe-S cluster transfer to target apo-proteins through CIA machinery in E. histolytica. Our study indicates that initial synthesis of a Fe-S precursor in mitochondria is not necessary for the formation of Cfd1-Nbp35 complex. Thus, Cfd1 and Nbp35 with the help of cytosolic NifS and NifU proteins can participate in the maturation of non-mitosomal Fe-S proteins without any

  13. Systematyzacja i pomiar postrzeganych zmian w obrazie siebie konsumentów pod wpływem korzystania z marek usług

    Directory of Open Access Journals (Sweden)

    Oleg Gorbaniuk

    2014-06-01

    Full Text Available Cel: Celami przeprowadzonych badań były (1 usystematyzowanie postrzeganych przez konsumentów pozytywnych i negatywnych zmian w obrazie siebie z tytułu korzystania z marek usług oraz (2 opracowanie wielowymiarowego narzędzia do pomiaru tych zmian. Metodologia: Aby osiągnąć powyższe cele, zrealizowano badania jakościowe oraz ilościowe. Badania jakościowe,prowadzone metodą indywidualnego wywiadu, objęły próbę 318 osób w wieku od 13 do 82 roku życia (M = 37,64, SD = 16,69; 47,2% kobiet. W efekcie skompletowano zbiór pozytywnych i negatywnych zmian w obrazie siebie jako konsumentów wskutek korzystania z usług różnych marek, które zostały poddane szczegółowej kategoryzacji. Opierając się na wynikach kategoryzacji, opracowano kwestionariusz, którego skale miały postać dyferencjału semantycznego. Badania ilościowe przeprowadzone zostały na próbie 378 osób w wieku od 15 do 78 lat (M = 34,62; SD = 13,42; 53,6% kobiet. Wnioski: Analiza wyników badań umożliwiła identyfikację dziewięciu wymiarów postrzeganych zmian w obrazie siebie pod wpływem korzystania z marek usług. Na podstawie uzyskanych wyników opracowano kwestionariusz do wielowymiarowego pomiaru zmian w obrazie siebie o bardzo dobrych wskaźnikach psychometrycznych z uwagi na rzetelność pomiaru i trafność przewidywania preferencji marek usług. Praktyczne implikacje: Opracowano nowatorskie wielowymiarowe narzędzie do praktycznego zastosowania w zarządzaniu marką usług. Zidentyfi kowane wymiary postrzeganych zmian obrazu siebie konsumentów marek stanowią praktyczną wskazówkę, czym należy się kierować w komunikacji marketingowej. Oryginalność: Wyniki badań i opracowane skale przełamują wieloletni impas w teorii i metodologii badań relacji Ja–marka.

  14. Catastrophic rotational braking among Sun-like stars. A model of the Sun's rotation evolution

    Science.gov (United States)

    Gondoin, P.

    2017-03-01

    Context. Observations of young open clusters show a bimodal distribution of stellar rotation. In those clusters, Sun-like stars group into two main populations of fast and slow rotators. Beyond an age of approximately 600 Myr, the two populations converge towards a single sequence of slow rotators. Aims: The present study addresses the origin of this bimodal distribution and the cause of its observed evolution. Methods: New prescriptions of mass-loss rate and Alfven radius dependences on Rossby number suggested by observations are implemented in a phenomenological model of angular-momentum loss and redistribution. The obtained model is used to calculate the time evolution of a rotation-period distribution of solar-mass stars similar to that observed in the 5 Myr-old NGC 2362 open cluster. The simulated distributions at subsequent ages are compared with those of h Per, the Pleiades, M 50, M 35, and M 37. Results: The model is able to reproduce the appearance and disappearance of a bimodal rotation-period distribution in open clusters providing that a brief episode of large-angular-momentum loss is included in the early evolution of Sun-like stars. Conclusions: I argue that a transitory episode of large-angular-momentum loss occurs on Sun-like stars with Rossby numbers between 0.13 and 0.3. This phenomenon of enhanced magnetic braking by stellar wind would be mainly driven by a rapid increase of mass loss at a critical rotation rate. This scenario accounts for the bimodal distribution of stellar rotation in open clusters with ages between 20-30 Myr and approximately 600 Myr. The mass-loss rate increase could account for a significant fraction of the X-ray luminosity decay of Sun-like stars in the 0.13-0.3 Rossby number range where a transition from the saturated to the non-saturated regime of X-ray emission is observed. Observed correlations between Li abundance and rotation sequences in the Pleiades and M 34 clusters support this scenario.

  15. Exploring individual differences in online and face-to-face help-seeking intentions in case of impending mental health problems: The role of adult attachment, perceived social support, psychological distress and self-stigma

    Directory of Open Access Journals (Sweden)

    Jennifer Apolinário-Hagen

    2016-11-01

    Full Text Available Background: Even though common mental health problems such as depression are a global burden calling for efficient prevention strategies, still many distressed individuals face hurdles to access public mental healthcare. Thus, computerized Internet-based psychological services have been suggested as viable approach to overcome barriers, such as self-stigma, and to inform the access to professional support on a large scale. However, little research has targeted predictors of online and face-to-face help-seeking intentions. Objective: This study aimed at determining whether associations between attachment insecurity and the willingness to seek online versus face-to-face counselling in case of impending emotional problems are mediated by both perceived social support and psychological distress and moderated by self-stigma. Methods: Data was collected from 301 adults from the German-speaking general population (age: M = 34.42, SD = 11.23; range: 18 - 65 years; 72.1% female through an anonymous online survey. Determinants of seeking help were assessed with the self-report measures Experiences in Close Relationship-Scale, Perceived Stress Questionnaire, ENRICHD-Social Support Inventory and an adapted version of the General Help Seeking Questionnaire (i.e. case vignette. Mediation analyses were performed with the SPSS-macro PROCESS by Hayes. Results: About half of the sample indicated being not aware of online counselling. As expected, insecure attachment was associated with less perceived social support and increased psychological distress. Mediational analyses revealed negative relationships between both attachment avoidance and self-stigma with face-to-face help-seeking intentions. Moreover, the relationship between attachment anxiety and the willingness to seek face-to-face counselling was mediated by social support. In contrast, none of the predictors of online counselling was statistically significant. Conclusions: Overall, this study identified

  16. Construction and Application of a Gateway Entry Vector pEN-L4*-PrbcS-*T-gfp-L3*%通路克隆入门载体pEN-L4*-PrbcS-*T-gfp-L3*的构建及其应用

    Institute of Scientific and Technical Information of China (English)

    肖素勤; 孙振; 王莎莎; 梁锋; 李莉; 年洪娟; 李昆志; 陈丽梅

    2011-01-01

    In order to construct the plant expression vector that contains two expression cassettes for two target genes through a Gateway LR recombination reaction and allows the expressed proteins encoded by the target genes to be localized to the chloroplasts of transgenic plants, Hind III and Xho I sites were generated in the Gateway entry vector, pEN-L4-2-L3, through a quickChange site-directed mutagenesis technology. Then a DNA fragment containing the tomato Rubisco small subunit 3C promoter (PrbcS) and its transit pep-tide sequence (*T) as well as a GFP (Green fluorescent protein) reporter gene (gfp) was inserted in the modified entry vector between the Hind III and Xho I sites to develop the entry vector pEN-L4*-PrbcS-*T-gfp-L3*. The plant expression vector pKm-35S-PrbcS-*T-gfp-PROLD-PrbcS-*T-gus containing the expression cassettes of gfp and gus genes could be constructed successfully with the developed entry vector pEN-L4*-PrbcS-*T-gfp-L3* and another Gateway entry vector (pENTR*-PrbcS-*T-gus) that contains attLl and attL2recombination sites as well as the plant destination vector pK7 m34GW2-8 m21GW3 through a Gateway LR reaction. Gfp and gus genes could be inserted in the genome of the transgenic plants and transcribed normally after the pKm-35S-PrbcS-*T-gfp-PROLD-PrbcS-*T-gus vector was used to transform tobacco. The analysis of GUS staining and GFP localization indicated that the GUS protein was expressed in the transgenic tobacco leaves and the expressed GFP protein was targeted to the chloroplasts of the leaves. Using the expression vector can not only complete the genetic manipulation of two target genes through one transformation but also locate the expressed proteins regulated by PrbcS promoter to the transgenic plant chloroplasts via the transit peptide sequence (*T). As a result, the construction and application of pEN-LA*-PibcS-*T-gfp-L3* entry vector establishes a technique platform and provides the convenience for chloroplast genetic engineering.%为

  17. Evaluation of Metal Toxicity in Streams Affected by Abandoned Mine Lands, Upper Animas River Watershed, Colorado

    Science.gov (United States)

    Besser, John M.; Allert, Ann L.; Hardesty, Douglas K.; Ingersoll, Christopher G.; May, Thomas W.; Wang, Ning; Leib, Kenneth J.

    2001-01-01

    water; (3) to develop site-specific thresholds for toxicity of Zn and Cu in stream water; and (4) to develop models of the contributions of Cu and Zn to toxicity of stream water, which may be used to characterize toxicity before and after planned remediation efforts. We evaluated the toxicity of metal-contaminated sediments by conducting sediment toxicity tests with two species of benthic invertebrates, the midge, Chironomus tentans. and the amphipod, Hyalella azteca. Laboratory toxicity tests with both taxa, exposed to fine stream-bed sediments collected in September 1997, showed some evidence of sediment toxicity, as survival of midge larvae in sediments from Cement Creek (C48) and lower Mineral Creek (M34), and growth of amphipods in sediments from these sites and three Animas River sites (A68, Animas at Silverton; A72, Animas below Silverton, and A73, Animas at Elk Park) were significantly reduced compared to a reference site, South Mineral Creek (SMC) . Amphipods were also exposed to site water and fine stream-bed sediment, separately and in combination, during the late summer low flow period (August-September) of 1998. In these studies, stream water, with no sediment present, from all five sites tested (same sites as above, except C48) caused 90% to 100% mortality of amphipods. In contrast, significant reductions in survival of amphipods occurred at two sites (A72 and SMC) in exposures with field-collected sediment plus stream water, and at only one site (A72) in exposures with sediments and clean overlying water. Concentrations of Zn, Pb, Cu, and Cd were high in both sediment and pore water (interstitial water) from most sites tested, but greatest sediment toxicity was apparently associated with greater concentrations of Fe and/or Al in sediments. These results suggest that fine stream-bed sediments of the more contaminated stream reaches of the upper Animas River watershed are toxic to benthic invertebrates, but that these impacts are less serious than tox

  18. ClassⅠ integron and its correlation with genes coding forESBLs in ESBLs-producing Escherichia coli from chil-dren%儿童产超广谱β-内酰胺酶大肠埃希菌Ⅰ类整合子及其与ESBLs基因关系的研究

    Institute of Scientific and Technical Information of China (English)

    蒋鸿超; 奎莉越; 黄海林; 苏敏; 樊茂

    2015-01-01

    ObjectiveTo study the correlation with Genes Coding forESBLs and ClassⅠIntegron in ESBLs-producing Escherichia coli from children.MethodsPCR was used for gene typing detection of 100 strains of ESBLs-producingEsche-richia colistrains. While detection of class I integrase gene in the 100 strains ESBLs-producingEscherichia coli and 100 strains of non-ESBLs producingEscherichia coli were separately performed by PCR. It provides the solid base not only to reveal the relationship between class I integron and drug resistance, but also the relationship between class I integron and ESBLs-producing. ResultsThe most frequently genotyping from ESBLs-producingEscherichia coli in children isCTX-M (84%), followed by TEM-1(63%). The predominant distribution of genotype in ESBL- producing strains isTEM-1 +CTX-M (45%), followed by CTX-M (34%). Class I integrase gene detected in ESBLs- producing and non- ESBLs producing strain were 100 cases (100%) and 25 cases (25%) separately, the difference was statistically signiifcant (P<0.05); drug resistance in class I integron positive strains were signiifcantly higher than in class I integron negative strains, especially in Ciprolfoxacin, Levolfoxacin, and Amino-glycoside (86.4%, 88%, and 80%).ConclusionsThe distribution of Class I integron in ESBLs-producingEscherichia coli is signiifcantly higher than that in non-ESBLs-producing strains, It is rational that Class I integron highly correlate with strong drug resistance in ESBLs-producing strains.%目的:研究儿童产超广谱β-内酰胺酶(ESBLs)大肠埃希菌I类整合子分布及其与ESBLs基因的相关性。方法采用PCR方法检测100株产ESBLs大肠埃希菌的ESBLs基因,以及100株产ESBLs与100株非产ESBLs大肠埃希菌的I类整合子基因,分析I类整合子与细菌耐药性的关系以及与ESBLs基因的关系。结果儿科临床产ESBLs大肠埃希菌基因分型以CTX-M型最多见(84.0%),其次为TEM-1型(63.0%);产ESBLs

  19. Morphological and Dyeing Characteristics of the Hemocytes of Paa boulengeri%棘腹蛙血细胞的形态特征及染色特性

    Institute of Scientific and Technical Information of China (English)

    周庆萍; 黄倩; 李松; 周雪林

    2011-01-01

    为了探讨棘腹蛙血细胞的形态结构,为两栖类动物生理学研究提供基础资料,采用Wright染色法对棘腹蛙血细胞的形态特征进行显微观察,测定了各形态参数.结果表明:棘腹蛙成熟的红细胞呈长椭圆形,长径33.59μm,短径17.73 μm;白细胞多呈圆形、椭圆形或不规则形.其中,嗜中性粒细胞长径27.12μm,短径19.72μm;嗜酸性粒细胞长径25.32 μm,短径22.63 μm;嗜碱性粒细胞长径20.26 μm,短径18.94 μm;单核细胞长径34.20 μm,短径21.67μm;小淋巴细胞长径14.38 μm,短径13.82 μm;大淋巴细胞长径20.44μm,短径18.31 μm;血栓细胞呈长梭形,长径32.74μm,短径14.53μm.%The observation of blood cells under the microscope and measurement of the morphological parameters of Pas boulengeri is processed by using the Wright staining with blood smear method to explore morphological structure of haemocytes and provide basic information for the study of amphibian physiology. The results show that maturing red blood cells of Pas boulengeri are ellipse in shape, long diameter is 33.59μm and short diameter is 17.73 μm; leukocytes are round or ellipse in shape, the long and short diameters of neutrophilic granulocytes, eosinophils, basophils, monocytes, small lymphocytes, big lymphocytes and thrombocytes are 27.12μm and 19.72μm, 25.32μm and 22.63 μm, 20.26 μm and 18.94 μm, 34.20μm and 21.67 μm, 14.38 μm and 13.82 μm, 20.44 μm and 18.31 μm, 32.74 μm and 14.53μm,respectively. And the thrombocytes are fusiform in shape.

  20. 高水位隧道衬砌临界水头数值计算%Numerical Calculation of High Water Head Tunnel on Critical Water Pressure

    Institute of Scientific and Technical Information of China (English)

    郑波; 吴剑; 王建宇

    2015-01-01

    Two kinds of typical tunnel section is chosen for analyzing the critical water pressure of high water head tunnel by numerical calculation method.The calculation results show that:①for the design speed 250 km/h double-line tunnel, when the lining thickness is respectively 30 cm, 50 cm, plain concrete lining is not liable for any water pressure load, and C30 reinforced concrete lining can bear respectively 11.2 m, 34.2 m water head load;②for the design speed 200 km/h single track railway tunnel, when the lining thickness is respectively 30 cm, 50 cm, 60 cm, plain concrete lining can bear respectively 0 m, 1 m, 5.5 m, and C30 reinforced concrete lin-ing can bear respectively 26 m, 55.2 m, 73.5 m water head load;③the tunnel shape has great influence on criti-cal water head, single-track railway tunnel can bear greater critical water pressure than double-track tunnel;④for high water head tunnel, according to new concept of water control in tunnel, the amount of water inflow should be limited.%选取2种典型隧道断面,对其所能承受的极限水头值进行分析。计算结果表明:①对设计时速250 km/h的客专双线隧道,当衬砌厚度分别为t=30 cm、50 cm时,素混凝土衬砌均不能承担任何水压力荷载,C30钢筋混凝土衬砌能承受的极限水位分别为11.2 m、34.2 m;②对设计时速200 km/h的单线铁路隧道,当衬砌厚度分别为30 cm、50 cm、60 cm时,素混凝土衬砌能承受的极限水头分别为0 m、1.0 m、5.5 m,C30钢筋混凝土衬砌能承受的极限水头分别为26.0 m、55.2 m、73.5 m;③隧道形状对所承受的极限水头值有较大的影响,单线铁路隧道比客专双线隧道更有利于承受水压力;④对于高水位隧道,应该采取“以堵为主,限量排放”的措施,降低衬砌水压力,使设计既安全又经济。

  1. Conhecimento dos pais sobre alimentação: construção e validação de um questionário de alimentação infantil Conocimiento de los padres sobre la alimentación: construcción y validación de un cuestionario sobre alimentación infantil Parents’ knowledge about feeding: construction and validation of a child feeding questionnaire

    Directory of Open Access Journals (Sweden)

    Maria da Graça ferreira Aparício Costa

    2012-03-01

    Full Text Available Enquadramento: instrumentos válidos que permitam obter informação sobre os conhecimentos e as práticas que medeiam a alimentação das crianças revelam-se de grande valor para o planeamento de ações de promoção da saúde mais dirigidas. Objetivo: descrever o processo de construção e validação dum questionário de avaliação de Conhecimentos dos Pais sobre Alimentação Infantil. Metodologia: participaram 559 pais de crianças pré-escolares, residentes no centro de Portugal, com média de idades, mãe (M= 34,43; Dp= 5,0; pai (M= 36,47; Dp=5,45 anos. Foi analisada a consistência interna e a validade de constructo. Resultados: o Alfa de Cronbach final, (Alfa=0,942, revelou boa consistência interna, com correlação positiva e significativa na nota global com e sem o item incluído. A análise fatorial de componentes principais, seguida de rotação varimax e gráfico scree plots, extraiu quatro fatores explicando 39,59% da variância total. A composição dos fatores sugere os significados: Fator 1 - Alimentação e Saúde; Fator 2 - Regras Alimentares; Fator 3 - Crenças Alimentares Familiares; Fator 4 - Variedade/Diversidade no Padrão Alimentar. Conclusão: as propriedades psicométricas do QAI certificam a sua qualidade, enquanto ferramenta a utilizar nas atividades de promoção da saúde, dirigida à identificação das necessidades de (informação dos pais sobre alimentação infantil.Antecedentes: instrumentos capaces de conseguir información acerca de los conocimientos y prácticas que intervienen en la alimentación de los niños, llegan a ser valioso para la planificación de acciones más centradas en la promoción de la salud. Objetivo: describir el proceso de construcción y validación de un cuestionario de evaluación del conocimiento de los padres sobre la alimentación infantil. Métodos: participaron 559 padres de niños en edad preescolar que viven en el centro de Portugal cuyas madres tienen una edad media de

  2. 耐药肺炎克雷伯菌β-内酰胺酶编码基因及KPC-ISKpn6连锁检测%Detection of β-lactamases encoding genes and K PC-ISK p n6 in drug-resistant Klebsiella pneumoniae

    Institute of Scientific and Technical Information of China (English)

    毛彩萍; 樊璠; 叶珍; 尤佳女; 奚伟星; 边佳; 糜祖煌; 陈文虎

    2014-01-01

    目的:研究和分析18株耐药肺炎克雷伯菌产β-内酰胺酶基因表达及KPC型阳性菌株与插入序列ISKpn6的关联。方法采用微生物鉴定仪鉴定并通过 gy rA、p arC基因测序和比对确认了18株肺炎克雷伯菌,对抗菌药物的药敏试验采用K-B纸片扩散法;40种β-内酰胺酶基因和K PC型β-内酰胺酶编码基因与插入序列(K PC-ISKpn6)连锁检测均采用PCR法,β-内酰胺酶编码基因的亚型通过测序和比对进行确认。结果18株耐药肺炎克雷伯菌除对四环素、米诺环素的敏感率>83.3%外,对其余抗菌药物的敏感率均<50.0%,有的甚至100.0%耐药;40种β-内酰胺酶基因中共检出 TEM-117株、CTX-M-34株、LAP-21株、KPC-214株、IMP-42株、DHA-14株、OXA-11株共7种耐药基因亚型,且基因检出与其细菌耐药表型相符合;14株 K PC-2型阳性菌株KPC-ISKpn6连锁检测均为阳性。结论耐药肺炎克雷伯菌每一株均有阳性基因检出,且检出的7种耐药基因亚型分布于β-内酰胺酶4个类别中,显示耐药株携带的β-内酰胺酶编码基因多种性;14株携带 K PC-2型基因的阳性株其KPC-ISKpn6连锁检测均为阳性,提示 K PC-2型β-内酰胺酶编码基因与插入序列ISKpn6高度关联。%OBJECTIVE To investigate the expression of β-lactamase genes in 18 strains of drug-resistant K lebsiella pneumoniae and study the correlation between KPC-positive strains and insertion sequence ISKpn6 .METHODS Totally 18 strains of K .pneumoniae have been identified through the microorganism identification system and the gyrA and parC gene sequencing ,then the drug susceptibility testing was performed by using K-B disk diffusion method ,the detection of 40β-lactamase genes ,KPC-typeβ-lactamase encoding genes and insertion sequence(KPC-ISKpn6) were performed by using PCR ,and the subtypes of the β-lactamases encoding genes were confirmed through sequencing and comparison

  3. La diabetes altera el potencial osteogénico de células progenitoras de médula ósea: Efectos del tratamiento con metformina Diabetes Decreases the Osteogenic Potential of Bone Marrow Progenitor Cells: Effects of Treatment with Metformin

    Directory of Open Access Journals (Sweden)

    MJ Tolosa

    2012-06-01

    Full Text Available En este trabajo, estudiamos el efecto de una Diabetes inducida por destrucción parcial de la masa de células beta pancreáticas, sobre el compromiso osteogénico de células progenitoras de médula ósea (CPMO, y su modulación por el tratamiento oral con Metformina. Para ello utilizamos ratas Sprague Dawley, divididas en cuatro grupos: controles [C], controles tratadas con Metformina [M], diabéticas [D], y diabéticas tratadas con Metformina [DM]. La inducción de Diabetes se realizó, por inyección intraperitoneal sucesiva de ácido nico-tínico y estreptozotocina. Sobre los cultivos de CPMO se evaluó la actividad específica de Fosfatasa Alcalina (FAL y la producción de Colágeno tipo 1 (Col-1 en estado basal y en medio de diferenciación osteogénico luego de 15 días. A los 21 días, se evaluaron los depósitos de mineral extracelular. La FAL y el Col-1 de CPMO basales, no mostraron diferencias significativas entre los cuatro grupos experimentales. Al cabo de 15 días, las CPMO de ratas M mostraron un incremento en el Col-1 de 122 % respecto de C; D 30 % respecto de C y DM 68 % respecto de C. La FAL expresó un 171 % para M, 34 % para D; y 125 % para DM todos respecto de C. Luego de 21 días, se observó una disminución en la mineralización de las CPMO de D (65 % respecto del grupo C. El tratamiento con metformina incrementó la mineralización de las CPMO en todos los casos. En conclusión, en nuestro modelo experimental de Diabetes, ésta disminuye el potencial osteogénico de las CPMO, un efecto que es parcialmente revertido por el tratamiento oral con Metformina. Estos hallazgos podrían explicar, al menos en parte, las alteraciones óseas descriptas en el hueso asociadas con la Diabetes. Los autores declaran no poseer conflictos de interés.Diabetes mellitus is associated with an increased incidence of skeletal abnormalities, resulting in lower bone formation and/or remodeling. Osteopenia, osteoporosis and an increased

  4. Video Electroencephalogram Characteristics of Different Seizure Types in Infants with Epilepsy%不同发作类型的婴幼儿癫(癎)视频脑电图特征

    Institute of Scientific and Technical Information of China (English)

    宋莉; 陈彦; 廖建湘; 陈黎; 徐雅娜; 路新国; 文家伦; 张端秀

    2012-01-01

    the disease were automatism or unilateral body stiffness jitter and attack duration lasted for 30 min or longer.5. Complex seizures in 43 cases, and VEEG showed their attacks transferred from one type into another in a single seizure. 6. The non - epileptic attacks in 372 cases, in which the attack happened during awake period in 214 cases, during sleep in 111 cases, during both sleep and awake in 47 cases. The prominent manifestations were slight shaking, and EEG showed movement artifact or no background changes. Conclusions VEEG monitor is helpful to the classification of seizures in infants with epilepsy. Epileptic spasms and partial seizures are main seizure types in infants younger than 2 years old. They must be differentiated from non - epileptic attacks,which are also common.%目的 通过视频脑电图(VEEG)分析癫(癎)婴幼儿的发作类型.方法 对深圳市儿童医院7 a来2岁以下且VEEG监测到发作性事件的1000例患儿的临床资料进行回顾性分析.结果 1.全面性发作41例.其中强直-阵挛发作4例,持续时间为(59.75±58.93)s,中位数(M)38.50 s.强直相持续(17.25±15.81)s.M12.50 s;阵挛相持续(35.00±29.54) s,M 29.00 s.发作期EEG主要表现为全导脑波抑制、全导棘波或低幅快活动发放后转变为棘波频率变慢夹杂慢波交替出现.强直发作6例,持续时间(36.66±16.97)s,M 34.00 s,发作期EEG为低中幅快节律持续发放.肌阵挛31例,持续时间(186.67±29.16) ms,M 200.00ms,发作期EEG为多尖棘慢波全导同发.2.痉挛发作302例.其中1岁内219例,1~2岁83例.发作持续时间为(1.35±0.67)s,M 1.00 s,发作间期背景EEG为高峰节律紊乱,其中210例为变异性高峰节律紊乱.临床表现屈肌型216例,伸肌型29例,混合型22例,眼球运动、轻微抖动等不典型发作35例.3.局限性发作232例.持续时间( 107.34±104.88)s,M 70.00 s.临床表现为强直抖动(阵挛)样发作或无功性凝视(肢体无明显动作,肌张力无改变),多