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Sample records for m34 casamino acids-yeast

  1. The manometric determination of thiamine pyrophosphate and the inhibition of the acid yeast phosphatase

    NARCIS (Netherlands)

    Steyn-Parvé, Elizabeth P.

    1962-01-01

    Sodium molybdate is a powerful inhibitor of the acid yeast phosphatase in both fresh baker's yeast and dried brewer's yeast, provided that the yeast is suspended in a suitable buffer. It displays no action in citrate or phosphate buffers, but is active in acetate or maleate buffers, both at the

  2. λ7774 Oxygen Triplet in Open Cluster Dwarfs: Pleiades and M34

    International Nuclear Information System (INIS)

    Schuler, S.C.; King, J.R.; Hobbs, L.M.; Pinsonneault, M.H.

    2005-01-01

    We have undertaken a LTE analysis of the high-excitation 7774 A O I triplet in high-resolution, moderate signal-to-noise spectra of 15 Pleiades (HET/HRS) and 8 M34 (Keck/HIRES) open cluster dwarfs. Effective temperatures range from 5048 - 6172 K for the Pleiades sample and from 5290 - 6130 K for the M34 sample. Relative O abundances have been derived using model atmospheres interpolated from four different sets of ATLAS9 grids: with convective overshoot, without convective overshoot, with the mixing length parameter set to 0.5, and with the convective treatment of Canuto, Goldman, and Mazzitelli. In contrast to existing NLTE predictions, a dramatic increase in O I triplet abundance with decreasing temperature is seen for both clusters, regardless of atmospheric model. S I abundances of three Pleiads derived from the high-excitation λ6052.67 feature mimic the O I abundance behavior. O abundances have also been derived from the 6300 A [OI] feature in three Pleiads; the abundances exhibit a much lower mean value than the cool dwarf triplet results. These data suggest LTE abundances derived from the O I triplet for cool dwarfs (T eff =<5800 K) should be viewed with caution

  3. Limited Multiplication of Symbiotic Cyanobacteria of Azolla spp. on Artificial Media

    Science.gov (United States)

    Tang, L. F.; Watanabe, I.; Liu, C. C.

    1990-01-01

    We examined various media and conditions to isolate symbiotic cyanobacteria from the leaf cavities of Azolla spp. Cyanobacteria survived and multiplied to a limited extent on a medium with fructose, Casamino Acids, yeast extract, and NaNO3 under 1% O2. These cyanobacteria were antigenically identical to the endosymbionts. Images PMID:16348366

  4. Tindallia Californiensis sp. nov.: A New Halo-Alkaliphilic Primary Anaerobe, Isolated from Meromictic soda Mono Lake in California and the Correction of Diagnosis for Genus Tindallia

    Science.gov (United States)

    Pikuta, Elena; Marsic, Damien; Hoover, Richard B.; Kevbrin, Vadim; Whitman, William B.; Krader, Paul; Cleland, Dave; Six, N. Frank (Technical Monitor)

    2002-01-01

    A novel extremely halo-alkaliphilic, bacterium strain APO (sup T) was isolated from sediments of the athalassic, meromictic, soda Mono Lake in California. Gram positive, spore-forming, slightly curved rods with sizes 0.6-0.7x 2.5-4.0 micrometers which occur singly, in pairs or short curved chains. Cells, are motile by singular subcentral flagellum. Strain APO (sup T) is mesophilic: growth was observed over the temperature range of +10 C to +48 C (optimum +37 C), NaCl concentration range 1-20 %, wt/vol (optimum 3-5%, wt/vol) and pH range 8.0-11.0 (optimum pH 9.5). The novel isolate is strictly halo-alkaliphilic, requires sodium chloride in medium, obligately anaerobic and catalase-negative. Strain APO (sup T) is organo-heterotroph with fermentative type of metabolism, and uses as substrates: peptone, badotryptone, casamino acids, yeast extract, L-serine, L-lysine, L-histidine, L-arginine, and pyruvate. The main end products of growth on peptone medium were: lactate, acetate, propionate, and ethanol. Strain APO (sup T) is resistant to kanamycin, but sensitive to chloramphenicol, tetracycline, and gentamycin. The sum of G+C in DNA is 44.4 mol% (by HPLC method). On the bait of physiological and molecular properties, the isolate was considered as novel species of genus Tindallia; and the name Tindallia californiensis sp. nov., is proposed for new isolate (type strain APO (sup T) - ATCC BAA_393(sup T) = DSMZ 14871 (sup T)).

  5. Antimutagenic action of aminoacids on UV-irradiated E. Coli cells: evidence of the existence of metabolic regulation of antimutagenic activity

    International Nuclear Information System (INIS)

    Filippov, V.D.

    1990-01-01

    The yield of mutations in Escherichia Coli cells placed after UV irradiation in a glucose-free salt medium enriched with casamino acids was determined. It is shown that in the absence of glucose casamino acids and certain individual amino acids produce a strong antimutagenetic effect. The acquired data allow to assume the existence of fine metabolic regulation of mutation reparation processes and occurrence of mutations in E. Coli cells exposed to UV-radiation

  6. Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

    CSIR Research Space (South Africa)

    Bulani, S

    2012-05-01

    Full Text Available (YlCWP1). mCherry was used as a model protein to assess the efficiency of the constructed plasmid. Y. lipolytica transformants harbouring the expression cassettes showed a purple colour phenotype on selective YNB-casamino plates as compared to control...

  7. Mutagenic DNA repair in Escherichia coli. Pt. 2

    International Nuclear Information System (INIS)

    Doubleday, O.P.; Bridges, B.A.; Green, M.H.L.

    1975-01-01

    The photoreversibility of UV-induced mutations to Trp + in strain Escherichia coli WP2 uvr A trp (unable to excise pyrimidine dimers) was lost at different rates during incubation in different media. In Casamino acids medium after a short initial lag, photoreversibility was lost over about one generation time; in minimal medium with tryptophan, photoreversibility persisted for more than two generations; in Casamino acids medium with pantoyl lactone photoreversibility was lost extremely slowly. The rate of loss of photoreversibility was unaffected by UV dose in either Casamino acids medium or in minimal medium. The same eventual number of induced mutants was obtained when cells were incubated for two generations in any of the three media before being transferred to selective plates supplemented with Casamino acids. Thus in each the proportion of cells capable of giving rise to a mutant was the same and only the rate at which these cells did so during post-irradiation growth varied, suggesting that there might be a specific fraction of pyrimidine dimers at a given site capable of initiating a mutagenic repair event, and that the size of this fraction is dose dependent. Segregation experiments have shown that error-prone repair appears to occur once only and is not repeated in subsequent replication cycles, in contrast to (presumed error-free) recombination repair. The results are discussed in the light of current models of UV mutagenesis. (orig.) [de

  8. Purification of heat labile toxin from Bordetella pertussis vaccine ...

    African Journals Online (AJOL)

    K.C. Shivanandappa

    2015-06-23

    Jun 23, 2015 ... heat inactivation at 56 °C for 30 min during Wcpv prepara- tion. Therefore, the ... The B2 culture medium was prepared with following composi- tions: Bacto casamino ... 15min at the ambient temperature (+18 to 22 °C). The.

  9. Mutagenic DNA repair in Escherichia coli. Pt. 2. Factors affecting loss of photoreversibility of UV induced mutations

    Energy Technology Data Exchange (ETDEWEB)

    Doubleday, O P; Bridges, B A; Green, M H.L. [Medical Research Council, Brighton (UK). Cell Mutation Unit

    1975-01-01

    The photoreversibility of UV-induced mutations to Trp/sup +/ in strain Escherichia coli WP2 uvr A trp (unable to excise pyrimidine dimers) was lost at different rates during incubation in different media. In Casamino acids medium after a short initial lag, photoreversibility was lost over about one generation time; in minimal medium with tryptophan, photoreversibility persisted for more than two generations; in Casamino acids medium with pantoyl lactone photoreversibility was lost extremely slowly. The rate of loss of photoreversibility was unaffected by UV dose in either Casamino acids medium or in minimal medium. The same eventual number of induced mutants was obtained when cells were incubated for two generations in any of the three media before being transferred to selective plates supplemented with Casamino acids. Thus in each the proportion of cells capable of giving rise to a mutant was the same and only the rate at which these cells did so during post-irradiation growth varied, suggesting that there might be a specific fraction of pyrimidine dimers at a given site capable of initiating a mutagenic repair event, and that the size of this fraction is dose dependent. Segregation experiments have shown that error-prone repair appears to occur once only and is not repeated in subsequent replication cycles, in contrast to (presumed error-free) recombination repair. The results are discussed in the light of current models of UV mutagenesis.

  10. Formation and conversion of oxygen metabolites by Lactococcus lactis subsp lactis ATCC 19435 under different growth conditions

    NARCIS (Netherlands)

    Niel, van E.W.J.; Hofvendahl, K.; Hahn Hagerdal, B.

    2002-01-01

    A semidefined medium based on Casamino Acids allowed Lactococcus lactis ATCC 19435 to grow in the presence of oxygen at a slow rate (0.015 h-1). Accumulation of H2O2 in the culture prevented a higher growth rate. Addition of asparagine to the medium increased the growth rate, whereby H2O2

  11. Sucrose Fermentation by Brazilian Ethanol Production Yeasts in Media Containing Structurally Complex Nitrogen Sources

    OpenAIRE

    Miranda Junior, Messias [UNESP; Batistote, Margareth [UNESP; Cilli, Eduardo Maffud [UNESP; Ernandes, Jose Roberto [UNESP

    2009-01-01

    Four Saccharomyces cerevisiae Brazilian industrial ethanol production strains were grown, under shaken and static conditions, in media containing 22% (w/v) sucrose supplemented with nitrogen sources varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids) and peptides (peptone). Sucrose fermentations by Brazilian industrial ethanol production yeasts strains were strongly affected by both the structural complexity of the nitrogen source and the availability o...

  12. Biomass production and secretion of hydrolytic enzymes are influenced by the structural complexity of the nitrogen source in Fusarium oxysporum and Aspergillus nidulans.

    Science.gov (United States)

    da Silva, M C; Bertolini, M C; Ernandes, J R

    2001-01-01

    The structural complexity of the nitrogen sources strongly affects biomass production and secretion of hydrolytic enzymes in filamentous fungi. Fusarium oxysporum and Aspergillus nidulans were grown in media containing glucose or starch, and supplemented with a nitrogen source varying from a single ammonium salt (ammonium sulfate) to free amino acids (casamino acids), peptides (peptone) and protein (gelatin). In glucose, when the initial pH was adjusted to 5.0, for both microorganisms, higher biomass production occurred upon supplementation with a nitrogen source in the peptide form (peptone and gelatin). With a close to neutrality pH, biomass accumulation was lower only in the presence of the ammonium salt. When grown in starch, biomass accumulation and secretion of hydrolytic enzymes (amylolytic and proteolytic) by Fusarium also depended on the nature of the nitrogen supplement and the pH. When the initial pH was adjusted to 5.0, higher growth and higher amylolytic activities were detected in the media supplemented with peptone, gelatin and casamino acids. However, at pH 7.0, higher biomass accumulation and higher amylolytic activities were observed upon supplementation with peptone or gelatin. Ammonium sulfate and casamino acids induced a lower production of biomass, and a different level of amylolytic enzyme secretion: high in ammonium sulfate and low in casamino acids. Secretion of proteolytic activity was always higher in the media supplemented with peptone and gelatin. Aspergillus, when grown in starch, was not as dependent as Fusarium on the nature of nitrogen source or the pH. The results described in this work indicate that the metabolism of fungi is regulated not only by pH, but also by the level of structural complexity of the nitrogen source in correlation to the carbon source.

  13. An altered Pseudomonas diversity is recovered from soil by using nutrient-poor Pseudomonas-selective soil extract media

    DEFF Research Database (Denmark)

    Aagot, N.; Nybroe, O.; Nielsen, P.

    2001-01-01

    We designed five Pseudomonas-selective soil extract NAA media containing the selective properties of trimethoprim and sodium lauroyl sarcosine and 0 to 100% of the amount of Casamino Acids used in the classical Pseudomonas-selective Gould's S1 medium. All of the isolates were confirmed to be Pseu......We designed five Pseudomonas-selective soil extract NAA media containing the selective properties of trimethoprim and sodium lauroyl sarcosine and 0 to 100% of the amount of Casamino Acids used in the classical Pseudomonas-selective Gould's S1 medium. All of the isolates were confirmed....... Several of these analyses showed that the amount of Casamino Acids significantly influenced the diversity of the recovered Pseudomonas isolates. Furthermore, the data suggested that specific Pseudomonas subpopulations were represented on the nutrient-poor media. The NAA 1:100 medium, containing ca. 15 mg...... of organic carbon per liter, consistently gave significantly higher Pseudomonas CFU counts than Gould's S1 when tested on four Danish soils. NAA 1:100 may, therefore, be a better medium than Gould's S1 for enumeration and isolation of Pseudomonas from the low-nutrient soil environment....

  14. Stabilization and preservation of probiotic properties of the ...

    African Journals Online (AJOL)

    This present study assessed the impact of drying process parameters, that is temperature and drying duration, on the dry matter content, pH, titratable acidity, yeasts and lactic acid bacteria content of granule starter of African opaque sorghum beer. Probiotic properties of the dry starter were tested. The aim was to establish ...

  15. Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor.

    Science.gov (United States)

    Hernández-Eugenio, Guadalupe; Fardeau, Marie-Laure; Cayol, Jean-Luc; Patel, Bharat K C; Thomas, Pierre; Macarie, Hervé; Garcia, Jean-Louis; Ollivier, Bernard

    2002-09-01

    A strictly anaerobic, gram-positive, sporulating rod (0.5-0.6 x 2.0-4.0 microm), designated strain Lup 21T, was isolated from an upflow anaerobic sludge blanket (UASB) reactor treating cheese-factory wastewater. Strain Lup 21T was motile by means of peritrichous flagella, had a G+C content of 31.4 mol% and grew optimally at 37 degrees C, pH 7.4, in the absence of NaCl. It is a heterotrophic micro-organism, utilizing proteinaceous compounds (gelatin, peptides, Casamino acids and various single amino acids) but unable to use any of the carbohydrates tested as a carbon and energy source. It reduced thiosulfate and elemental sulfur to sulfide in the presence of Casamino acids as carbon and energy sources. Acetate, butyrate, isobutyrate, isovalerate, CO2 and sulfide were end products from oxidation of gelatin and Casamino acids in the presence of thiosulfate as an electron acceptor. In the absence of thiosulfate, serine, lysine, methionine and histidine were fermented. On the basis of 16S rRNA similarity, strain Lup 21T was related to members of the low-G+C Clostridiales group, Clostridium subterminale DSM 6970T being the closest relative (with a sequence similarity of 99.4%). DNA-DNA hybridization was 56% with this species. On the basis of phenotypic, genotypic and phylogenetic characteristics, the isolate was designated as a novel species of the genus Clostridium, Clostridium thiosulfatireducens sp. nov. The type strain is strain Lup 21T (= DSM 13105T = CIP 106908T).

  16. Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations

    DEFF Research Database (Denmark)

    Ödman, Peter; Johansen, C.L.; Olsson, L.

    2010-01-01

    of biomass and substrate (casamino acids) concentrations, respectively. The effect of combination of fluorescence and gas analyzer data as well as of different variable selection methods was investigated. Improved prediction models were obtained by combination of data from the two sensors and by variable......Fed-batch cultivations of Streptomyces coelicolor, producing the antibiotic actinorhodin, were monitored online by multiwavelength fluorescence spectroscopy and off-gas analysis. Partial least squares (PLS), locally weighted regression, and multilinear PLS (N-PLS) models were built for prediction...

  17. Nutrients interaction investigation to improve Monascus purpureus FTC5391 growth rate using Response Surface Methodology and Artificial Neural Network

    Directory of Open Access Journals (Sweden)

    Mohamad, R.

    2013-01-01

    Full Text Available Aims: Two vital factors, certain environmental conditions and nutrients as a source of energy are entailed for successful growth and reproduction of microorganisms. Manipulation of nutritional requirement is the simplest and most effectual strategy to stimulate and enhance the activity of microorganisms. Methodology and Results: In this study, response surface methodology (RSM and artificial neural network (ANN were employed to optimize the carbon and nitrogen sources in order to improve growth rate of Monascus purpureus FTC5391,a new local isolate. The best models for optimization of growth rate were a multilayer full feed-forward incremental back propagation network, and a modified response surface model using backward elimination. The optimum condition for cell mass production was: sucrose 2.5%, yeast extract 0.045%, casamino acid 0.275%, sodium nitrate 0.48%, potato starch 0.045%, dextrose 1%, potassium nitrate 0.57%. The experimental cell mass production using this optimal condition was 21 mg/plate/12days, which was 2.2-fold higher than the standard condition (sucrose 5%, yeast extract 0.15%, casamino acid 0.25%, sodium nitrate 0.3%, potato starch 0.2%, dextrose 1%, potassium nitrate 0.3%. Conclusion, significance and impact of study: The results of RSM and ANN showed that all carbon and nitrogen sources tested had significant effect on growth rate (P-value < 0.05. In addition the use of RSM and ANN alongside each other provided a proper growth prediction model.

  18. Growth of Campylobacter incubated aerobically in fumarate-pyruvate media or media supplemented with dairy, meat, or soy extracts and peptones.

    Science.gov (United States)

    Hinton, Arthur

    2016-09-01

    The ability of Campylobacter to grow aerobically in media supplemented with fumarate-pyruvate or with dairy, meat, or soy extracts or peptones was examined. Optical densities (OD) of Campylobacter cultured in basal media, media supplemented with fumarate-pyruvate or with 1.0, 2.5, 5.0, or 7.5% beef extract was measured. Growth was also compared in media supplemented with other extracts or peptones. Finally, cfu/mL of Campylobacter recovered from basal media or media supplemented with fumarate-pyruvate, casamino acids, beef extract, soytone, or beef extract and soytone was determined. Results indicated that OD of cultures grown in media supplemented with fumarate-pyruvate or with 5.0 or 7.5% beef extract were higher than OD of isolates grown in basal media or media supplemented with lower concentrations of beef extract. Highest OD were produced by isolates grown in media supplemented with beef extract, peptone from meat, polypeptone, proteose peptone, or soytone. Also, more cfu/mL were recovered from media with fumarate-pyruvate, beef extract, soytone, or beef extract-soytone than from basal media or media with casamino acids. Findings indicate that media supplemented with organic acids, vitamins, and minerals and media supplemented with extracts or peptones containing these metabolites can support aerobic growth of Campylobacter. Published by Elsevier Ltd.

  19. Stigmatellin Y - An anti-biofilm compound from Bacillus subtilis BR4 possibly interferes in PQS-PqsR mediated quorum sensing system in Pseudomonas aeruginosa.

    Science.gov (United States)

    Boopathi, Seenivasan; Vashisth, Rajesh; Manoharan, Prabu; Kandasamy, Ruckmani; Sivakumar, Natesan

    2017-05-15

    Hitherto this is the first report pertaining to production of biofilm inhibitory compound(s) (BIC) from Bacillus subtilis BR4 against Pseudomonas aeruginosa (ATCC 27853) coupled with production optimization. In order to achieve this, combinations of media components were formulated by employing statistical tools such as Plackett-Burman analysis and central composite rotatable design (CCRD). It was evident that at 35mlL -1 glycerol and 3.8gL -1 casamino acid, anti-biofilm activity and production of extracellular protein significantly increased by 1.5-fold and 1.2-fold, respectively. These results corroborate that the combination of glycerol and casamino acid plays a key role in the production of BIC. Further, metabolic profiling of BIC was carried out using liquid chromatography/tandem mass spectrometry (LC-MS/MS) based on m/z value. The presence of Stigmatellin Y was predicted with monoisotopic neutral mass of 484.2825Da. In support of optimization study, higher production of BIC was confirmed in the optimized-media-grown BR4 (OPT-BR4) than in the ideal-media-grown BR4 (ID-BR4) by LC-MS/MS analysis. PqsR in P. aeruginosa is a potential target for anti-virulent therapy. Molecular docking study has revealed that Stigmatellin Y interacts with PqsR in the similar orientation like a cognate signal (PQS) and synthetic inhibitor. In addition, Stigmatellin Y was found to exhibit interaction with four more amino acid residues of PqsR to establish strong affinity. Stigmatellin Y thus might play a role of competitor for PQS to distract PQS-PqsR mediated communication in P. aeruginosa. The present investigation thus paves new avenues to develop anti-Pseudomonas virulent therapy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeasts species

    Science.gov (United States)

    Sitepu, Irnayuli R.; Sestric, Ryan; Ignatia, Laura; Levin, David; German, J. Bruce; Gillies, Laura A.; Almada, Luis A.G.; Boundy-Mills, Kyria L.

    2013-01-01

    Oleaginous yeasts have been studied for oleochemical production for over 80 years. Only a few species have been studied intensely. To expand the diversity of oleaginous yeasts available for lipid research, we surveyed a broad diversity of yeasts with indicators of oleaginicity including known oleaginous clades, and buoyancy. Sixty-nine strains representing 17 genera and 50 species were screened for lipid production. Yeasts belonged to Ascomycota families, Basidiomycota orders, and the yeast-like algal genus Prototheca. Total intracellular lipids and fatty acid composition were determined under different incubation times and nitrogen availability. Thirteen new oleaginous yeast species were discovered, representing multiple ascomycete and basidiomycete clades. Nitrogen starvation generally increased intracellular lipid content. The fatty acid profiles varied with the growth conditions regardless of taxonomic affiliation. The dominant fatty acids were oleic acid, palmitic acid, linoleic acid, and stearic acid. Yeasts and culture conditions that produced fatty acids appropriate for biodiesel were identified. PMID:23891835

  1. Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species.

    Science.gov (United States)

    Sitepu, Irnayuli R; Sestric, Ryan; Ignatia, Laura; Levin, David; German, J Bruce; Gillies, Laura A; Almada, Luis A G; Boundy-Mills, Kyria L

    2013-09-01

    Oleaginous yeasts have been studied for oleochemical production for over 80 years. Only a few species have been studied intensely. To expand the diversity of oleaginous yeasts available for lipid research, we surveyed a broad diversity of yeasts with indicators of oleaginicity including known oleaginous clades, and buoyancy. Sixty-nine strains representing 17 genera and 50 species were screened for lipid production. Yeasts belonged to Ascomycota families, Basidiomycota orders, and the yeast-like algal genus Prototheca. Total intracellular lipids and fatty acid composition were determined under different incubation times and nitrogen availability. Thirteen new oleaginous yeast species were discovered, representing multiple ascomycete and basidiomycete clades. Nitrogen starvation generally increased intracellular lipid content. The fatty acid profiles varied with the growth conditions regardless of taxonomic affiliation. The dominant fatty acids were oleic acid, palmitic acid, linoleic acid, and stearic acid. Yeasts and culture conditions that produced fatty acids appropriate for biodiesel were identified. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Utilization of whey

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    Compositions of wheys from cheesemaking and ultrafiltration are tabulated and some indications of the pollution potential and the cost of disposal of whey are given. The following treatments are described: ion exchange and electrodialysis for demineralization and deacidification; concentration by reverse osmosis, evaporation, spray drying, ''Hatmaker'' (roller) drying, and a drying technique using small Teflon balls; separation of proteins by ''Centri-Whey'' (Alfa-Laval) and ''Bel-Industrie'' heat/acid coagulation, ultrafiltration and other methods; purification and enzymic hydrolysis of lactose, and use of lactose as a substrate for protein biosynthesis by ''Bel'' (lactic acid yeast), ''Devos'' (Saccharomyces yeast) and ''Caliqua-Sireb'' (using fungi) processes. The use of whey for producing ethanol, lactic acid, vinegar, vitamins, antibiotics, enzymes and fats; and development of industrial whey processing in France are reviewed.

  3. Nitrogen recycling from fuel-extracted algal biomass: residuals as the sole nitrogen source for culturing Scenedesmus acutus.

    Science.gov (United States)

    Gu, Huiya; Nagle, Nick; Pienkos, Philip T; Posewitz, Matthew C

    2015-05-01

    In this study, the reuse of nitrogen from fuel-extracted algal residues was investigated. The alga Scenedesmus acutus was found to be able to assimilate nitrogen contained in amino acids, yeast extracts, and proteinaceous alga residuals. Moreover, these alternative nitrogen resources could replace nitrate in culturing media. The ability of S. acutus to utilize the nitrogen remaining in processed algal biomass was unique among the promising biofuel strains tested. This alga was leveraged in a recycling approach where nitrogen is recovered from algal biomass residuals that remain after lipids are extracted and carbohydrates are fermented to ethanol. The protein-rich residuals not only provided an effective nitrogen resource, but also contributed to a carbon "heterotrophic boost" in subsequent culturing, improving overall biomass and lipid yields relative to the control medium with only nitrate. Prior treatment of the algal residues with Diaion HP20 resin was required to remove compounds inhibitory to algal growth. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. Utilization of whey

    Energy Technology Data Exchange (ETDEWEB)

    1979-01-01

    Compositions of wheys from cheesemaking and ultrafiltration are tabulated and some indications of the pollution potential and the cost of disposal of whey are given. The following treatments are described: ion exchange and electrodialysis for demineralization and deacidification; concentration by reverse osmosis, evaporation, spray drying, ''Hatmaker'' (roller) drying, and a drying technique using small Teflon balls; separation of proteins by ''Centri-Whey'' (Alfa-Laval) and ''Bel-Industrie'' heat/acid coagulation, ultrafiltration and other methods; purification and enzymic hydrolysis of lactose, and use of lactose as a substrate for protein biosynthesis by ''Bel'' (lactic acid yeast), ''Devos'' (Saccharomyces yeast) and ''Caliqua-Sireb'' (using fungi) processes. The use of whey for producing ethanol, lactic acid, vinegar, vitamins, antibiotics, enzymes and fats; and development of industrial whey processing in France are reviewed.

  5. Regulatory role of OsMADS34 in the determination of glumes fate, grain yield and quality in rice

    Directory of Open Access Journals (Sweden)

    Deyong Ren

    2016-12-01

    Full Text Available Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we used m34-z, a new mutant allele of the rice (Oryza sativa E-class gene OsMADS34, to examine OsMADS34 function in determining the identities of glumes (rudimentary glume and sterile lemma and grain size. In the m34-z mutant, both the rudimentary glume and sterile lemma were homeotically converted to the lemma-like organs and acquired the lemma identity, suggesting that OsMADS34 plays important roles in the development of glumes. In the m34-z mutant, most of the grains from the secondary panicle branches were decreased in size, compared with grains from wild type, but no differences were observed in the grains from the primary panicle branches. The amylose content and gel consistency, and a seed-setting rate from the secondary panicle branches were reduced in the m34-z mutant. Interesting, transcriptional activity analysis revealed that OsMADS34 protein was a transcription repressor and it may influence grain yield by suppressing the expressions of BG1, GW8, GW2 and GL7 in the m34-z mutant. These findings revealed that OsMADS34 largely affects grain yield by affecting the size of grains from the secondary branches.

  6. Mouse lung adhesion assay for Bordetella pertussis

    Energy Technology Data Exchange (ETDEWEB)

    Burns, K A; Freer, J H [Department of Microbiology, Alexander Stone Building, Bearsden, Glasgow, Scotland

    1982-03-01

    The ability of Bordetella pertussis to adhere to cell surfaces has been demonstrated by adhesion to tissue culture cells and adhesion to chicken, hamster or rabbit trachea in organ culture. In this report a mouse lung assay for adhesion is described and the results obtained using two virulent strains of B. pertussis and their avirulent counterparts. These were a C modulation of one of the original virulent strains and a phase IV variant of the other virulent strain. Organisms were radiolabelled by adding 1 ..mu..Ci (37 K Bq) of (/sup 14/C)glutamic acid per 10 ml of culture medium before inoculation and incubation for 5 days. The lungs were washed by perfusion in situ with at least two volumes (1 ml) of sterile 1% (w/v) casamino acids. The percentage of the inoculated organisms retained in the lungs was determined, after removal of the lungs, by one of the following two methods: viable count or radioactive count. Results for both methods were expressed as the percentage of the inoculum retained in the lungs plus or minus one standard deviation.

  7. Proline-rich peptide from the coral pathogen Vibrio shiloi that inhibits photosynthesis of Zooxanthellae.

    Science.gov (United States)

    Banin, E; Khare, S K; Naider, F; Rosenberg, E

    2001-04-01

    The coral-bleaching bacterium Vibrio shiloi biosynthesizes and secretes an extracellular peptide, referred to as toxin P, which inhibits photosynthesis of coral symbiotic algae (zooxanthellae). Toxin P was produced during the stationary phase when the bacterium was grown on peptone or Casamino Acids media at 29 degrees C. Glycerol inhibited the production of toxin P. Toxin P was purified to homogeneity, yielding the following 12-residue peptide: PYPVYAPPPVVP (molecular weight, 1,295.54). The structure of toxin P was confirmed by chemical synthesis. In the presence of 12.5 mM NH(4)Cl, pure natural or synthetic toxin P (10 microM) caused a 64% decrease in the photosynthetic quantum yield of zooxanthellae within 5 min. The inhibition was proportional to the toxin P concentration. Toxin P bound avidly to zooxanthellae, such that subsequent addition of NH(4)Cl resulted in rapid inhibition of photosynthesis. When zooxanthellae were incubated in the presence of NH(4)Cl and toxin P, there was a rapid decrease in the pH (pH 7.8 to 7.2) of the bulk liquid, suggesting that toxin P facilitates transport of NH(3) into the cell. It is known that uptake of NH(3) into cells can destroy the pH gradient and block photosynthesis. This mode of action of toxin P can help explain the mechanism of coral bleaching by V. shiloi.

  8. Diversities in virulence, antifungal activity, pigmentation and DNA fingerprint among strains of Burkholderia glumae.

    Science.gov (United States)

    Karki, Hari S; Shrestha, Bishnu K; Han, Jae Woo; Groth, Donald E; Barphagha, Inderjit K; Rush, Milton C; Melanson, Rebecca A; Kim, Beom Seok; Ham, Jong Hyun

    2012-01-01

    Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly correlated to virulence on onion bulb scales, suggesting that onion bulb can be a convenient alternative host system to efficiently determine the virulence of B. glumae strains. Production of toxoflavin, the phytotoxin that functions as a major virulence factor, was closely associated with the virulence phenotypes of B. glumae strains in rice. Some strains of B. glumae showed various levels of antifungal activity against Rhizoctonia solani, the causal agent of sheath blight, and pigmentation phenotypes on casamino acid-peptone-glucose (CPG) agar plates regardless of their virulence traits. Purple and yellow-green pigments were partially purified from a pigmenting strain of B. glumae, 411gr-6, and the purple pigment fraction showed a strong antifungal activity against Collectotrichum orbiculare. Genetic variations were detected among the B. glumae strains from DNA fingerprinting analyses by repetitive element sequence-based PCR (rep-PCR) for BOX-A1R-based repetitive extragenic palindromic (BOX) or enterobacterial repetitive intergenic consensus (ERIC) sequences of bacteria; and close genetic relatedness among virulent but pigment-deficient strains were revealed by clustering analyses of DNA fingerprints from BOX-and ERIC-PCR.

  9. A PEG-DA microfluidic device for chemotaxis studies

    International Nuclear Information System (INIS)

    Traore, Mahama Aziz; Behkam, Bahareh

    2013-01-01

    The study of cells in a well-defined and chemically programmable microenvironment is essential for a complete and fundamental understanding of the cell behaviors with respect to specific chemical compounds. Flow-free microfluidic devices that generate quasi-steady chemical gradients (spatially varying but temporally constant) have been demonstrated as effective chemotaxis assay platforms due to dissociating the effect of chemical cues from mechanical shear forces caused by fluid flow. In this work, we demonstrate the fabrication and characterization of a flow-free microfluidic platform made of polyethylene glycol diacrylate (PEG-DA) hydrogel. We have demonstrated that the mass transport properties of these devices can be customized by fabricating them from PEG-DA gels of four distinct molecular weights. In contrast to microfluidic devices developed using soft lithography; this class of devices can be realized using a more cost-effective approach of direct photopolymerization with fewer microfabrication steps. This microfluidic platform was tested by conducting a quantitative study of the chemotactic behavior of Escherichia coli (E. coli) RP437, a model microorganism, in presence of the chemo-effector, casamino-acids. Using the microfabrication and characterization methodology presented in this work, microfluidic platforms with well-defined and customizable diffusive properties can be developed to accommodate the study of a wide range of cell types. (paper)

  10. Factors stimulating riboflavin produced by Lactobacillus plantarum CRL 725 grown in a semi-defined medium.

    Science.gov (United States)

    Juarez Del Valle, Marianela; Laiño, Jonathan Emiliano; Savoy de Giori, Graciela; LeBlanc, Jean Guy

    2017-03-01

    Riboflavin (vitamin B 2 ) is one of the B-group water-soluble vitamins and is essential for energy metabolism of the cell. The aim of this study was to determine factors that affect riboflavin production by Lactobacillus (L.) plantarum CRL 725 grown in a semi defined medium and evaluate the expression of its rib genes. The factors found to enhance riboflavin production in this medium were incubation at 30 °C, and the addition of specific medium constituents, such as casamino acids (10 g L -1 ), guanosine (0.04 g L -1 ), and sucrose as carbon source (20 g L -1 ). In these conditions, higher riboflavin concentrations were directly associated with significant increases in the expression of ribA, ribB, and ribC genes. The culture conditions defined in this work and its application to a roseoflavin resistant mutant of L. plantarum allowed for a sixfold increase in riboflavin concentrations in our semi-defined medium which were also significantly higher than those obtained previously using the same strain to ferment soymilk. These conditions should thus be evaluated to increase vitamin production in fermented foods. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. ISOLATION AND CHARACTERIZATION OF A NOVEL BENZOATE- UTILIZING Serratia marcescens

    Directory of Open Access Journals (Sweden)

    ANTONIUS SUWANTO

    2003-01-01

    Full Text Available A new benzoate-utilizing strain, Serratia marcescens DS-8, isolated from the environment was characterized. The strain was enterobacilli, Gram negative, mesophilic, non ha lophilic, and aerobic bacterium that showed motile ovale-rod shaped cells. The isolate produced extracellular chitinase, protease, and prodigiosin (a red pigment pr oduced by several Serratia strains yielding bright red or pink colonies. A physiological assay using Microbact* test showed that the strain was closely related to Klebsiella ozaenae (49.85% and Serratia liquefaciens (24.42%, respectively. However, 16S rRNA sequence analysis indicated that the strain was closely related to S. marcescens DSM 30121 with similarity level of 98%. DS-8 strain was able to synthesize its own vitamins. Optimum growth in benzoate was obtained at pH between 7-8.5 and NaCl concentration of 1- 1.5% (w/v. The isolate could grow in benzoate-containing medium up to 10 mM. Other carbon sources that could support the growth of DS-8 were casamino acid, glutamate, glucose, acetate, potato star ch, and ethanol.

  12. Portable hyperspectral fluorescence imaging system for detection of biofilms on stainless steel surfaces

    Science.gov (United States)

    Jun, Won; Lee, Kangjin; Millner, Patricia; Sharma, Manan; Chao, Kuanglin; Kim, Moon S.

    2008-04-01

    A rapid nondestructive technology is needed to detect bacterial contamination on the surfaces of food processing equipment to reduce public health risks. A portable hyperspectral fluorescence imaging system was used to evaluate potential detection of microbial biofilm on stainless steel typically used in the manufacture of food processing equipment. Stainless steel coupons were immersed in bacterium cultures, such as E. coli, Pseudomonas pertucinogena, Erwinia chrysanthemi, and Listeria innocula. Following a 1-week exposure, biofilm formations were assessed using fluorescence imaging. In addition, the effects on biofilm formation from both tryptic soy broth (TSB) and M9 medium with casamino acids (M9C) were examined. TSB grown cells enhance biofilm production compared with M9C-grown cells. Hyperspectral fluorescence images of the biofilm samples, in response to ultraviolet-A (320 to 400 nm) excitation, were acquired from approximately 416 to 700 nm. Visual evaluation of individual images at emission peak wavelengths in the blue revealed the most contrast between biofilms and stainless steel coupons. Two-band ratios compared with the single-band images increased the contrast between the biofilm forming area and stainless steel coupon surfaces. The 444/588 nm ratio images exhibited the greatest contrast between the biofilm formations and stainless coupon surfaces.

  13. Factors affecting capsule size and production by lactic acid bacteria used as dairy starter cultures.

    Science.gov (United States)

    Hassan, A N; Frank, J F; Shalabi, S I

    2001-02-28

    The effects of sugar substrates on capsule size and production by some capsule-forming nonropy and ropy dairy starter cultures were studied. Test sugars (glucose, lactose, galactose, or sucrose) were used as a sole carbohydrate source and the presence of a capsule and its size were determined by using confocal scanning laser microscopy. Nonropy strains produced maximum capsule size when grown in milk. Strains that did not produce capsules in milk did not produce them in any other growth medium. Specific sugars required for capsule production were strain-dependent. Increasing lactose content of Elliker broth from 0.5 to 5% or adding whey protein or casein digest produced larger capsules. Whey protein concentrate stimulated production of larger capsules than did casamino acids or casitone. Some Streptococcus thermophilus strains produced capsules when grown on galactose only. Nonropy strains of Lactobacillus delbrueckii subsp. bulgaricus produced capsules on lactose, but not on glucose. A ropy strain of Lactobacillus delbrueckii subsp. bulgaricus produced a constant capsule size regardless of the growth medium. The ability of some strains of Streptococcus thermophilus to use galactose in capsule production could reduce browning of mozzarella cheese during baking by removing a source of reducing sugar. Media that do not support capsule production may improve cell harvesting.

  14. Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathway.

    Science.gov (United States)

    Kosalková, Katarina; García-Estrada, Carlos; Barreiro, Carlos; Flórez, Martha G; Jami, Mohammad S; Paniagua, Miguel A; Martín, Juan F

    2012-01-10

    The secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway. Using the secretion of bovine chymosin in Aspergillus awamori as a model, we found a drastic increase (40 to 80-fold) in cells grown with casein or casein phosphopeptides (CPPs). CPPs are rich in phosphoserine, but phosphoserine itself did not increase the secretion of chymosin. The stimulatory effect is reduced about 50% using partially dephosphorylated casein and is not exerted by casamino acids. The phosphopeptides effect was not exerted at transcriptional level, but instead, it was clearly observed on the secretion of chymosin by immunodetection analysis. Proteomics studies revealed very interesting metabolic changes in response to phosphopeptides supplementation. The oxidative metabolism was reduced, since enzymes involved in fermentative processes were overrepresented. An oxygen-binding hemoglobin-like protein was overrepresented in the proteome following phosphopeptides addition. Most interestingly, the intracellular pre-protein enzymes, including pre-prochymosin, were depleted (most of them are underrepresented in the intracellular proteome after the addition of CPPs), whereas the extracellular mature form of several of these secretable proteins and cell-wall biosynthetic enzymes was greatly overrepresented in the secretome of phosphopeptides-supplemented cells. Another important 'moonlighting' protein (glyceraldehyde-3-phosphate dehydrogenase), which has been described to have vesicle fusogenic and cytoskeleton formation modulating activities, was clearly overrepresented in phosphopeptides-supplemented cells. In summary, CPPs cause the reprogramming of cellular metabolism, which leads to massive secretion of extracellular proteins.

  15. Levels of cystathionine gamma lyase production by Geotrichum candidum in synthetic media and correlation with the presence of sulphur flavours in cheese.

    Science.gov (United States)

    Gente, Stéphanie; La Carbona, Stéphanie; Guéguen, Micheline

    2007-03-10

    Geotrichum candidum is a cheese-ripening agent with the potential to produce sulphur flavour compounds in soft cheeses. We aimed to develop an alternative test for predicting the aromatic (sulphur flavours) potential of G. candidum strains in soft cheese. Twelve strains of G. candidum with different levels of demethiolase activity (determined by a chemical method) in YEL-met (yeast extract, lactate methionine) medium were studied. We investigated cgl (cystathionine gamma lyase) gene expression after culture in three media - YEL-met, casamino acid and curd media - and then carried out sensory analysis on a Camembert cheese matrix. We found no correlation between demethiolase activity in vitro and cgl gene expression. Sensory analysis (detection of sulphur flavours) identified different aromatic profiles linked to cgl expression, but not to demethiolase activity. The RT-PCR technique described here is potentially useful for predicting the tendency of a given strain of G. candidum to develop sulphur flavours in cheese matrix. This is the first demonstration that an in vitro molecular approach could be used as a predictive test for evaluating the potential of G. candidum strains to generate sulphur compounds in situ (Camembert cheese matrix).

  16. Carbon Concentration and Carbon-to-Nitrogen Ratio Influence Submerged-Culture Conidiation by the Potential Bioherbicide Colletotrichum truncatum NRRL 13737

    Science.gov (United States)

    Jackson, Mark A.; Bothast, Rodney J.

    1990-01-01

    We assessed the influence of various carbon concentrations and carbon-to-nitrogen (C:N) ratios on Colletotrichum truncatum NRRL 13737 conidium formation in submerged cultures grown in a basal salts medium containing various amounts of glucose and Casamino Acids. Under the nutritional conditions tested, the highest conidium concentrations were produced in media with carbon concentrations of 4.0 to 15.3 g/liter. High carbon concentrations (20.4 to 40.8 g/liter) inhibited sporulation and enhanced the formation of microsclerotiumlike hyphal masses. At all the carbon concentrations tested, a culture grown in a medium with a C:N ratio of 15:1 produced more conidia than cultures grown in media with C:N ratios of 40:1 or 5:1. While glucose exhaustion was often coincident with conidium formation, cultures containing residual glucose sporulated and those with high carbon concentrations (>25 g/liter) exhausted glucose without sporulation. Nitrogen source studies showed that the levels of C. truncatum NRRL 13737 conidiation were similar for all protein hydrolysates tested. Reduced conidiation occurred when amino acid and inorganic nitrogen sources were used. Of the nine carbon sources evaluated, acetate as the sole carbon source resulted in the lowest level of sporulation. Images PMID:16348348

  17. Deferribacter thermophilus gen. nov., sp. nov., a novel thermophilic manganese- and iron-reducing bacterium isolated from a petroleum reservoir.

    Science.gov (United States)

    Greene, A C; Patel, B K; Sheehy, A J

    1997-04-01

    A thermophilic anaerobic bacterium, designated strain BMAT (T = type strain), was isolated from the production water of Beatrice oil field in the North Sea (United Kingdom). The cells were straight to bent rods (1 to 5 by 0.3 to 0.5 microns) which stained gram negative. Strain BMAT obtained energy from the reduction of manganese (IV), iron(III), and nitrate in the presence of yeast extract, peptone, Casamino Acids, tryptone, hydrogen, malate, acetate, citrate, pyruvate, lactate, succinate, and valerate. The isolate grew optimally at 60 degrees C (temperature range for growth, 50 to 65 degrees C) and in the presence of 2% (wt/vol) NaCl (NaCl range for growth, 0 to 5% [wt/vol]). The DNA base composition was 34 mol% G + C. Phylogenetic analyses of the 16S rRNA gene indicated that strain BMAT is a member of the domain Bacteria. The closest known bacterium is the moderate thermophile Flexistipes sinusarabici (similarity value, 88%). Strain BMAT possesses phenotypic and phylogenetic traits that do not allow its classification as a member of any previously described genus; therefore, we propose that this isolate should be described as a member of a novel species of a new genus, Deferribacter thermophilus gen. nov., sp. nov.

  18. Effects of different nitrogen sources on the biogas production - a lab-scale investigation.

    Science.gov (United States)

    Wagner, Andreas Otto; Hohlbrugger, Peter; Lins, Philipp; Illmer, Paul

    2012-12-20

    For anaerobic digestion processes nitrogen sources are poorly investigated although they are known as possible process limiting factors (in the hydrolysis phase) but also as a source for fermentations for subsequent methane production by methanogenic archaea. In the present study different complex and defined nitrogen sources were investigated in a lab-scale experiment in order to study their potential to build up methane. The outcome of the study can be summarised as follows: from complex nitrogen sources yeast extract and casamino acids showed the highest methane production with approximately 600 ml methane per mole of nitrogen, whereas by the use of skim milk no methane production could be observed. From defined nitrogen sources L-arginine showed the highest methane production with almost 1400 ml methane per mole of nitrogen. Moreover it could be demonstrated that the carbon content and therefore C/N-ratio has only minor influence for the methane production from the used substrates. Copyright © 2011 Elsevier GmbH. All rights reserved.

  19. Effects of UPR and ERAD pathway on the prolyl endopeptidase production in Pichia pastoris by controlling of nitrogen source.

    Science.gov (United States)

    Wang, Xiao-Dong; Jiang, Ting; Yu, Xiao-Wei; Xu, Yan

    2017-07-01

    Prolyl endopeptidase (PEP) is very useful in various industries, while the high cost of enzyme production remains a major obstacle for its industrial applications. Pichia pastoris has been used for the PEP production; however, the fermentation process has not be investigated and little is known about the impact of excessive PEP production on the host cell physiology. Here, we optimized the nitrogen source to improve the PEP expression level and further evaluated the cellular response including UPR and ERAD. During methanol induction phase the PEP activity (1583 U/L) was increased by 1.48-fold under the optimized nitrogen concentration of NH 4 + (300 mmol/L) and casamino acids [1.0% (w/v)] in a 3-L bioreactor. Evaluated by RT-PCR the UPR and ERAD pathways were confirmed to be activated. Furthermore, a strong decrease of ERAD-related gene transcription was observed with the addition of nitrogen source, which contributed to a higher PEP expression level.

  20. Mouse lung adhesion assay for Bordetella pertussis

    International Nuclear Information System (INIS)

    Burns, K.A.; Freer, J.H.

    1982-01-01

    The ability of Bordetella pertussis to adhere to cell surfaces has been demonstrated by adhesion to tissue culture cells and adhesion to chicken, hamster or rabbit trachea in organ culture. In this report a mouse lung assay for adhesion is described and the results obtained using two virulent strains of B. pertussis and their avirulent counterparts. These were a C modulation of one of the original virulent strains and a phase IV variant of the other virulent strain. Organisms were radiolabelled by adding 1 μCi (37 K Bq) of [ 14 C]glutamic acid per 10 ml of culture medium before inoculation and incubation for 5 days. The lungs were washed by perfusion in situ with at least two volumes (1 ml) of sterile 1% (w/v) casamino acids. The percentage of the inoculated organisms retained in the lungs was determined, after removal of the lungs, by one of the following two methods: viable count or radioactive count. Results for both methods were expressed as the percentage of the inoculum retained in the lungs plus or minus one standard deviation. (Auth.)

  1. Late Bombardment of the Lunar Highlands Recorded in MIL 090034, MIL 090036 and MIL 090070 Lunar Meteorites

    Science.gov (United States)

    Park, J.; Nyquist, L. E.; Shih, C.-Y.; Herzog, G. F.; Yamaguchi, A.; Shirai, N.; Ebihara, M.; Lindsay, F. N.; Delaney, J.; Turrin, B.; hide

    2013-01-01

    The Kaguya mission detected small but widespread outcrops of nearly pure ferroan anorthosite in and around large impact basins on the Moon. Along with certain lunar rocks, highly feldspathic lunar meteorites such as MIL 090034 (M34), 090036 (M36), and 090070 (M70) may provide samples of this material. We have measured the Ar-40/Ar-39 release patterns and cosmogenic Ar-38 concentrations of several small (<200 microg) samples separated from M34,36, and 70. From petrographic observations concluded that "some of the clasts and grains experienced generations of modifications," a conclusion that we examine in light of our data.

  2. USSR Report, Cybernetics, Computers and Automation Technology

    Science.gov (United States)

    1987-03-31

    version of the system was tested by adapting PAL-11 and MACRO-11 assembly code for the "Elektronika=60" and "Elektronika-60M&#34 computers; ASM -86 for the...GS, "On the Results of Evaluation of Insurance Payments in Collective and State Farms and Private Households," the actuarial analysis tables based

  3. Travelling Bubble Cavitation and Resulting Noise.

    Science.gov (United States)

    1981-03-02

    pp. 22-26, 1968. 16. Il’ichev, V. I. "Statistical Model of the Onset of Hydrodynamic Cavitation Noise," Sixth All-Union Acoustic Conference...Collected Papers, Moscow, 1968. 17. Lyamshev, L. M. &#34On the Theory of Hydrodynamic Cavitation Noise," Soviet Physics-Acoustics, Vol. 15, pp. 494-498, 1970. 18

  4. The Effects of Pilot Age, Lighting, and Head-Down Time on Visual Accommodation

    Science.gov (United States)

    1979-02-06

    technique for determining the extent of ametropia (nearsighted- ness, farsightedness, astigmatism) and as an aid in the fitting (8.1)of eyeglasses. Knoll... Ametropia with a Gas LASER", "Amerinan 1onu~nl nf Onpw’rp"r-_ Vol. 43, No. 7: 415-418, 1966. 4. Oliver, G. M. &#34Sparkling Spots and Random Diffractions

  5. An Empirical Study of Logistics Organization, Electronic Linkage, and Performance

    Science.gov (United States)

    1993-01-01

    Anvari, M., &#34Electronic Data Interchange and Inventories," International Journal of Production Economics , 26, 1-3 (1992), 135-143. Baker, R. H., EDI: What...Electronic Data Interchange -- A Study of Norwegian Freight Forwarders Using EDI," International Journal of Production Economics , 24 (1991), 91-101. Henry, G

  6. Workshop Proceedings: Toughening Mechanisms in Quasi-Brittle Materials Held on 16-20 July 1990 in Evanston, Illinois

    Science.gov (United States)

    1991-05-05

    2] Planas, J. and Elices, M., &#34Un Nuevo Mdtodo de An6lisis del Comportamiento Asint6tico de la Propagaci6n de una Fisura Cohesiva en Modo I...cohesive crack in mode I loading", Report 87-02, Departamento de Ciencia de Materiales , ETS de Ingenieros de Caminos, Ciudad Universitaria, 28040 Madrid

  7. NATO Advanced Research Workshop. Toughening Mechanisms in Quasi-Brittle Materials, Held in Evanston, Illinois on July 16-20, 1990

    Science.gov (United States)

    1990-07-20

    Planas, J. and Elices, M., &#34Un Nuevo Mdtodo de An~lisis del Comportamiento Asint6tico de la Propagaci6n de una Fisura Cohesiva en Modo ", Anales de...crack in mode I loading", Report 87-02, Departamento de Ciencia de Materiales , ETS de Ingenieros de Caminos, Ciudad Universitaria, 28040 Madrid, Spain

  8. Fundamental Studies in Blow-Down and Cryogenic Cooling

    Science.gov (United States)

    1993-09-01

    Mudawar , I. and Anderson, T.M., -High Flux Electronic Cooling by Means of Pool Boiling - Part I: Parametric Investigation of the Effects of Coolant...Electronics, pp. 25-34, 1989. 30 Mudawar , I. and Anderson, T.M., &#34High Flux Electronic Cooling by Means of Pool Boiling - Part 1I: Optimization of

  9. Validation of Flight Critical Control Systems

    Science.gov (United States)

    1991-12-01

    1985. [8] Avizienis, A., and Lyu, M., &#34On the Effectiveness of Multiversion Software in Digital Avionics", AIAA Computers in Aerospace VI Conference...Experimentation and Modelling. NASA CR-165036, 1982. [12] Eckhardt, D. E.; and Lee, L. D.: A Theoretical Basis for the Analysis of Multiversion

  10. Mechanical Face Seal Dynamics.

    Science.gov (United States)

    1985-12-01

    1473, 83 APR EDITION OF I JAN 73 IS OBSOLETE. UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGE -,1 - " P V 7 V - • ... f -N- PRE FACE This final...dimensionless mass m and support damping 1), ~ at-e aisas M&#34= -1,,i -4 4) y positive. ’he damping D is Ihe tinplete system of momeints acting on tile

  11. Hybrid Robust Multi-Objective Evolutionary Optimization Algorithm

    Science.gov (United States)

    2009-03-10

    xfar by xint. Else, generate a new individual, using the Sobol pseudo- random sequence generator within the upper and lower bounds of the variables...12. Deb, K., Multi-Objective Optimization Using Evolutionary Algorithms, John Wiley & Sons. 2002. 13. Sobol , I. M., &#34Uniformly Distributed Sequences

  12. Adiabatic Compression Sensitivity of AF-M315E

    Science.gov (United States)

    2015-07-01

    Brand for their technical expertise and guidance. He also wishes to thank Mr. Stephen McKim from NASA Goddard Space Flight Center for his assistance...Wilson, D. B., and Stoltzfus, J. M. &#34Adiabatic Compression of Oxygen: Real Fluid Temperatures," 2000. 10Ismail, I. M. K., and Hawkins , T. W. "Adiabatic

  13. Mechanics of Brittle Materials. Part 1. Preliminary Mechanical Properties and Statistical Representations

    Science.gov (United States)

    1973-10-01

    intensity computation are shown in Figure 17. Using the same formal procedure outlined by Winne & Wundt . a notch geometry can be chosen to induce...Nitride at Elevated Temperatures . Winne, D.H. and Wundt , B.M., &#34Application of the Gnffith-Irwm Theory of Crack Propagation to the Bursting Behavior

  14. Development of Metamaterial Composites for Compact High Power Microwave Systems and Antennas

    Science.gov (United States)

    2016-05-01

    Silva Neto, L.P.; de Siqueira, R.H.M. &#34Study of the ceramic capacitor dielectric for pulsed power", Pulsed Power Conference (PPC), 2013 19th IEEE...47 5.3.5 Barium Titanate Ferrite Composites ................................................................. 50 6. Dielectric ...Strength Measurement ..................................................................................... 59 6.1 Design of Pulsed Dielectric Breakdown

  15. Evaluation of Specialized Photoacoustic Absorption Chambers for Near-Millimeter Wave (NMMW) Propagation Measurements.

    Science.gov (United States)

    1980-08-01

    traveling a distance of only 8 inches. 3B. L. Bean and S. Perkowitz, 1976, "Far Infrared Transmission Measurements with an Optical Pumped FIR Laser," Appl ...32 ......... I k.4 102. D’Arcy, Edward M., &#34Accuracy Validation of the Modified Nike Hercules Radar," ASL-TP-J031, May 1979. 103. Rodriguez, Ruben

  16. Task Force Smith and the 24th Infantry Division in Korea, July 1950

    Science.gov (United States)

    2014-05-22

    11D.M. Giangreco, "Artillery in Korea: Massing Fires and Reinventing the Wheel," (Korean War Anthology , Combat...and General Staff College, 2000. Giangreco, D.M. &#34Artillery in Korea: Massing Fires and Reinventing the Wheel." Korean War Anthology , Combat

  17. Self-Concept: The Interplay of Theory and Methods.

    Science.gov (United States)

    1981-04-01

    Matrix," Psychological Bulletin, 1959, 56, 81-105. Coopersmith , S., The Antecedents of Self-esteem, San Francisco: Free- man, 1967. Fernandes, L., "The...Casuality, New York: Wiley, 1979. Kokenes, B. M., &#34A Factor Analytic Study of the Coopersmith Self Esteem Inventory," Dissertation Abstacts

  18. A conserved two-component regulatory system, PidS/PidR, globally regulates pigmentation and virulence-related phenotypes of Burkholderia glumae.

    Science.gov (United States)

    Karki, Hari Sharan; Barphagha, Inderjit Kaur; Ham, Jong Hyun

    2012-09-01

    Burkholderia glumae is a rice pathogenic bacterium that causes bacterial panicle blight. Some strains of this pathogen produce dark brown pigments when grown on casamino-acid peptone glucose (CPG) agar medium. A pigment-positive and highly virulent strain of B. glumae, 411gr-6, was randomly mutagenized with mini-Tn5gus, and the resulting mini-Tn5gus derivatives showing altered pigmentation phenotypes were screened on CPG agar plates to identify the genetic elements governing the pigmentation of B. glumae. In this study, a novel two-component regulatory system (TCRS) composed of the PidS sensor histidine kinase and the PidR response regulator was identified as an essential regulatory factor for pigmentation. Notably, the PidS/PidR TCRS was also required for the elicitation of the hypersensitive response on tobacco leaves, indicating the dependence of the hypersensitive response and pathogenicity (Hrp) type III secretion system of B. glumae on this regulatory factor. In addition, B. glumae mutants defective in the PidS/PidR TCRS showed less production of the phytotoxin, toxoflavin, and less virulence on rice panicles and onion bulbs relative to the parental strain, 411gr-6. The presence of highly homologous PidS and PidR orthologues in other Burkholderia species suggests that PidS/PidR-family TCRSs may exert the same or similar functions in different Burkholderia species, including both plant and animal pathogens. © 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD.

  19. A proteome analysis of the response of a Pseudomonas aeruginosa oxyR mutant to iron limitation.

    Science.gov (United States)

    Vinckx, Tiffany; Wei, Qing; Matthijs, Sandra; Noben, Jean-Paul; Daniels, Ruth; Cornelis, Pierre

    2011-06-01

    In Pseudomonas aeruginosa the response to oxidative stress is orchestrated by the LysR regulator OxyR by activation of the transcription of two catalase genes (katA and katB), of the alkyl-hydroxyperoxidases ahpCF and ahpB. Next to the expected high sensitivity to oxidative stress generated by reactive oxygen species (ROS: H(2)O(2), O(2)(-)), the oxyR mutant shows a defective growth under conditions of iron limitation (Vinckx et al. 2008). Although production and uptake of the siderophore pyoverdine is not affected by the absence of oxyR, the mutant is unable to satisfy its need for iron when grown under iron limiting conditions. In order to get a better insight into the effects caused by iron limitation on the physiological response of the oxyR mutant we decided to compare the proteomes of the wild type and the mutant grown in the iron-poor casamino acids medium (CAA), in CAA plus H(2)O(2), and in CAA plus the strong iron chelator ethylenediamine-N,N'-bis(2-hydroxyphenylacetic acid) (EDDHA). Especially in the presence of hydrogen peroxide the oxyR cells increase the production of stress proteins (Dps and IbpA). The superoxide dismutase SodM is produced in higher amounts in the oxyR mutant grown in CAA plus H(2)O(2). The PchB protein, a isochorismate-pyruvate lyase involved in the siderophore pyochelin biosynthesis is not detectable in the extracts from the oxyR mutant grown in the presence of hydrogen peroxide. When cells were grown in the presence of EDDHA, we observed a reduction of the ferric uptake regulator (Fur), and an increase in the two subunits of the succinyl-CoA synthetase and the fumarase FumC1.

  20. Induction of L-form-like cell shape change of Bacillus subtilis under microculture conditions.

    Science.gov (United States)

    Shingaki, Ryuji; Kasahara, Yasuhiro; Iwano, Megumi; Kuwano, Masayoshi; Takatsuka, Tomomasa; Inoue, Tetsuyoshi; Kokeguchi, Susumu; Fukui, Kazuhiro

    2003-09-01

    A remarkable cell shape change was observed in Bacillus subtilis strain 168 under microculture conditions on CI agar medium (Spizizen's minimal medium supplemented with a trace amount of yeast extract and Casamino acids). Cells cultured under a cover glass changed in form from rod-shaped to spherical, large and irregular shapes that closely resembled L-form cells. The cell shape change was observed only with CI medium, not with Spizizen's minimum medium alone or other rich media. The whole-cell protein profile of cells grown under cover glass and cells grown on CI agar plates differed in several respects. Tandem mass analysis of nine gel bands which differed in protein expression between the two conditions showed that proteins related to nitrate respiration and fermentation were expressed in the shape-changed cells grown under cover glass. The cell shape change of CI cultures was repressed when excess KNO3 was added to the medium. Whole-cell protein analysis of the normal rod-shaped cells grown with 0.1% KNO3 and the shape-changed cells grown without KNO3 revealed that the expression of the branched-chain alpha-keto acid dehydrogenase complex (coded by the bfmB gene locus) was elevated in the shape-changed cells. Inactivation of the bfmB locus resulted in the repression of cell shape change, and cells in which bfmB expression was induced by IPTG did show changes in shape. Transmission electron microscopy of ultrathin sections demonstrated that the shape-changed cells had thin walls, and plasmolysis of cells fixed with a solution including 0.1 M sucrose was observed. Clarifying the mechanism of thinning of the cell wall may lead to the development of a new type of cell wall biosynthetic inhibitor.

  1. Isolation and Characterization of Proteolytic Ruminal Bacteria from Sheep and Goats Fed the Tannin-Containing Shrub Legume Calliandra calothyrsus

    Science.gov (United States)

    McSweeney, Christopher S.; Palmer, Brian; Bunch, Rowan; Krause, Denis O.

    1999-01-01

    Tannins in forages complex with protein and reduce the availability of nitrogen to ruminants. Ruminal bacteria that ferment protein or peptides in the presence of tannins may benefit digestion of these diets. Bacteria from the rumina of sheep and goats fed Calliandra calothyrsus (3.6% N and 6% condensed tannin) were isolated on proteinaceous agar medium overlaid with either condensed (calliandra tannin) or hydrolyzable (tannic acid) tannin. Fifteen genotypes were identified, based on 16S ribosomal DNA-restriction fragment length polymorphism analysis, and all were proteolytic and fermented peptides to ammonia. Ten of the isolates grew to high optical density (OD) on carbohydrates (glucose, cellobiose, xylose, xylan, starch, and maltose), while the other isolates did not utilize or had low growth on these substrates. In pure culture, representative isolates were unable to ferment protein that was present in calliandra or had been complexed with tannin. One isolate, Lp1284, had high protease activity (80 U), a high specific growth rate (0.28), and a high rate of ammonia production (734 nmol/min/ml/OD unit) on Casamino Acids and Trypticase Peptone. Phylogenetic analysis of the 16S ribosomal DNA sequence showed that Lp1284 was related (97.6%) to Clostridium botulinum NCTC 7273. Purified plant protein and casein also supported growth of Lp1284 and were fermented to ammonia. This is the first report of a proteolytic, ammonia-hyperproducing bacterium from the rumen. In conclusion, a diverse group of proteolytic and peptidolytic bacteria were present in the rumen, but the isolates could not digest protein that was complexed with condensed tannin. PMID:10388706

  2. Sulfur Metabolism of Hydrogenovibrio thermophilus Strain S5 and Its Adaptations to Deep-Sea Hydrothermal Vent Environment

    Directory of Open Access Journals (Sweden)

    Lijing Jiang

    2017-12-01

    Full Text Available Hydrogenovibrio bacteria are ubiquitous in global deep-sea hydrothermal vents. However, their adaptations enabling survival in these harsh environments are not well understood. In this study, we characterized the physiology and metabolic mechanisms of Hydrogenovibrio thermophilus strain S5, which was first isolated from an active hydrothermal vent chimney on the Southwest Indian Ridge. Physiological characterizations showed that it is a microaerobic chemolithomixotroph that can utilize sulfide, thiosulfate, elemental sulfur, tetrathionate, thiocyanate or hydrogen as energy sources and molecular oxygen as the sole electron acceptor. During thiosulfate oxidation, the strain produced extracellular sulfur globules 0.7–6.0 μm in diameter that were mainly composed of elemental sulfur and carbon. Some organic substrates including amino acids, tryptone, yeast extract, casamino acids, casein, acetate, formate, citrate, propionate, tartrate, succinate, glucose and fructose can also serve as carbon sources, but growth is weaker than under CO2 conditions, indicating that strain S5 prefers to be chemolithoautotrophic. None of the tested organic carbons could function as energy sources. Growth tests under various conditions confirmed its adaption to a mesophilic mixing zone of hydrothermal vents in which vent fluid was mixed with cold seawater, preferring moderate temperatures (optimal 37°C, alkaline pH (optimal pH 8.0, microaerobic conditions (optimal 4% O2, and reduced sulfur compounds (e.g., sulfide, optimal 100 μM. Comparative genomics showed that strain S5 possesses more complex sulfur metabolism systems than other members of genus Hydrogenovibrio. The genes encoding the intracellular sulfur oxidation protein (DsrEF and assimilatory sulfate reduction were first reported in the genus Hydrogenovibrio. In summary, the versatility in energy and carbon sources, and unique physiological properties of this bacterium have facilitated its adaptation to deep

  3. Members of native coral microbiota inhibit glycosidases and thwart colonization of coral mucus by an opportunistic pathogen.

    Science.gov (United States)

    Krediet, Cory J; Ritchie, Kim B; Alagely, Ali; Teplitski, Max

    2013-05-01

    The outcome of the interactions between native commensal microorganisms and opportunistic pathogens is crucial to the health of the coral holobiont. During the establishment within the coral surface mucus layer, opportunistic pathogens, including a white pox pathogen Serratia marcescens PDL100, compete with native bacteria for available nutrients. Both commensals and pathogens employ glycosidases and N-acetyl-glucosaminidase to utilize components of coral mucus. This study tested the hypothesis that specific glycosidases were critical for the growth of S. marcescens on mucus and that their inhibition by native coral microbiota reduces fitness of the pathogen. Consistent with this hypothesis, a S. marcescens transposon mutant with reduced glycosidase and N-acetyl-glucosaminidase activities was unable to compete with the wild type on the mucus of the host coral Acropora palmata, although it was at least as competitive as the wild type on a minimal medium with glycerol and casamino acids. Virulence of the mutant was modestly reduced in the Aiptasia model. A survey revealed that ∼8% of culturable coral commensal bacteria have the ability to inhibit glycosidases in the pathogen. A small molecular weight, ethanol-soluble substance(s) produced by the coral commensal Exiguobacterium sp. was capable of the inhibition of the induction of catabolic enzymes in S. marcescens. This inhibition was in part responsible for the 10-100-fold reduction in the ability of the pathogen to grow on coral mucus. These results provide insight into potential mechanisms of commensal interference with early colonization and infection behaviors in opportunistic pathogens and highlight an important function for the native microbiota in coral health.

  4. Mutation induction by 365-nm radiation and far-ultraviolet light in Escherichia coli differing in near- and far-ultraviolet light sensitivity

    International Nuclear Information System (INIS)

    Leonardo, J.M.; Reynolds, P.R.; Tuveson, R.W.

    1984-01-01

    The his-4 locus derived from Escherichia coli strain AB1157 has been transduced into 4 E. coli strains that exhibit all 4 possible combinations of genes controlling sensitivity to near-ultraviolet light (nur versus nur + ) and far-ultraviolet light (uvrA6 versus uvrA + ). The 4 strains exhibited the predicted sensitivity to 254-nm radiation based on the sensitivity of the parent strains from which they were derived and the frequency of his + mutations predicted from experiments with AB1157 from which the his-4 locus was derived. When the 4 strains were treated with 365-nm radiation, they exhibited the predicted sensitivity based on the near-ultraviolet light sensitivity of the strains from which they were derived while his + mutations were undetectable with the 4 strains as well as with strain AB1157. When treated with 365-nm radiation, cells of a WP2sub(s) strain (a derivative of B/r transduced to his-4) plated on semi-enriched medium prepared with casamino acids did not yield induced mutations, whereas plating on semi-enriched medium prepared with nutrient broth did yield mutants at both the his-4 and trp loci at frequencies at least an order of magnitude lower than that observed with far-ultraviolet light. The induction of nutritionally independent mutants by 365-nm radiation is strongly dependent on the supplement used for semi-enrichment. When compared at equivalent survival levels, mutant frequencies are significantly less following 365-nm radiation when compared with far-ultraviolet radiation. (Auth.)

  5. Expression and characterization of the antimicrobial peptide ABP-dHC-cecropin A in the methylotrophic yeast Pichia pastoris.

    Science.gov (United States)

    Sang, Ming; Wei, Hui; Zhang, Jiaxin; Wei, Zhiheng; Wu, Xiaolong; Chen, Yan; Zhuge, Qiang

    2017-12-01

    ABP-dHC-cecropin A is a linear cationic peptide that exhibits antimicrobial properties. To explore a new approach for expression of ABP-dHC-cecropin A using the methylotrophic yeast Pichia pastoris, we cloned the ABP-dHC-cecropin A gene into the vector pPICZαA. The SacI-linearized plasmid pPICZαA-ABP-dHC-cecropin A was then transformed into P. pastoris GS115 by electroporation. Expression was induced after a 96-h incubation with 0.5% methanol at 20 °C in a culture supplied with 2% casamino acids to avoid proteolysis. Under these conditions, approximately 48 mg of ABP-dHC-cecropin A was secreted into 1L (4 × 250-mL)of medium. Recombinant ABP-dHC-cecropin A was purified using size-exclusion chromatography, and 21 mg of pure active ABP-dHC-cecropin A was obtained from 1L (4 × 250-mL)of culture. Electrophoresis on 4-20% gradient gels indicated that recombinant ABP-dHC-cecropin A was secreted as a protein approximately 4 kDa in size. Recombinant ABP-dHC-cecropin A was successfully expressed, as the product displayed antibacterial and antifungal activities (based on an antibacterial assay, scanning electron microscopy, and antifungal assay) indistinguishable from those of the synthesized protein. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Casein phosphopeptides drastically increase the secretion of extracellular proteins in Aspergillus awamori. Proteomics studies reveal changes in the secretory pathway

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    Kosalková Katarina

    2012-01-01

    Full Text Available Abstract Background The secretion of heterologous animal proteins in filamentous fungi is usually limited by bottlenecks in the vesicle-mediated secretory pathway. Results Using the secretion of bovine chymosin in Aspergillus awamori as a model, we found a drastic increase (40 to 80-fold in cells grown with casein or casein phosphopeptides (CPPs. CPPs are rich in phosphoserine, but phosphoserine itself did not increase the secretion of chymosin. The stimulatory effect is reduced about 50% using partially dephosphorylated casein and is not exerted by casamino acids. The phosphopeptides effect was not exerted at transcriptional level, but instead, it was clearly observed on the secretion of chymosin by immunodetection analysis. Proteomics studies revealed very interesting metabolic changes in response to phosphopeptides supplementation. The oxidative metabolism was reduced, since enzymes involved in fermentative processes were overrepresented. An oxygen-binding hemoglobin-like protein was overrepresented in the proteome following phosphopeptides addition. Most interestingly, the intracellular pre-protein enzymes, including pre-prochymosin, were depleted (most of them are underrepresented in the intracellular proteome after the addition of CPPs, whereas the extracellular mature form of several of these secretable proteins and cell-wall biosynthetic enzymes was greatly overrepresented in the secretome of phosphopeptides-supplemented cells. Another important 'moonlighting' protein (glyceraldehyde-3-phosphate dehydrogenase, which has been described to have vesicle fusogenic and cytoskeleton formation modulating activities, was clearly overrepresented in phosphopeptides-supplemented cells. Conclusions In summary, CPPs cause the reprogramming of cellular metabolism, which leads to massive secretion of extracellular proteins.

  7. Thermosyntropha lipolytica gen. nov., sp. nov., a lipolytic, anaerobic, alkalitolerant, thermophilic bacterium utilizing short- and long-chain fatty acids in syntrophic coculture with a methanogenic archaeum.

    Science.gov (United States)

    Svetlitshnyi, V; Rainey, F; Wiegel, J

    1996-10-01

    Three strains of an anaerobic thermophilic organoheterotrophic lipolytic alkalitolerant bacterium, Thermosyntropha lipolytica gen. nov., sp. nov. (type strain JW/VS-265T; DSM 11003), were isolated from alkaline hot springs of Lake Bogoria (Kenya). The cells were nonmotile, non-spore forming, straight or slightly curved rods. At 60 degrees C the pH range for growth determined at 25 degrees C [pH25 degrees C] was 7.15 to 9.5, with an optimum between 8.1 and 8.9 (pH60 degrees C of 7.6 and 8.1). At a pH25 degrees C of 8.5 the temperature range for growth was from 52 to 70 degrees C, with an optimum between 60 and 66 degrees C. The shortest doubling time was around 1 h. In pure culture the bacterium grew in a mineral base medium supplemented with yeast extract, tryptone, Casamino Acids, betaine, and crotonate as carbon sources, producing acetate as a major product and constitutively a lipase. During growth in the presence of olive oil, free long-chain fatty acids were accumulated in the medium but the pure culture could not utilize olive oil, triacylglycerols, short- and long-chain fatty acids, and glycerol for growth. In syntrophic coculture (Methanobacterium strain JW/VS-M29) the lipolytic bacteria grew on triacylglycerols and linear saturated and unsaturated fatty acids with 4 to 18 carbon atoms, but glycerol was not utilized. Fatty acids with even numbers of carbon atoms were degraded to acetate and methane, while from odd-numbered fatty acids 1 mol of propionate per mol of fatty acid was additionally formed. 16S rDNA sequence analysis identified Syntrophospora and Syntrophomonas spp. as closest phylogenetic neighbors.

  8. Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia

    Energy Technology Data Exchange (ETDEWEB)

    Cole, Jesse; Gieler, Brandon; Heisler, Devon; Palisoc, Maryknoll; Williams, Amanda; Dohnalkova, Alice; Ming, Hong; Yu, Tian T.; Dodsworth, Jeremy A.; Li, Wen J.; Hedlund, Brian P.

    2013-08-15

    Several closely-related, thermophilic, and cellulolytic bacterial strains, designated JKG1T, JKG2, JKG3, JKG4, and JKG5, were isolated from a cellulolytic enrichment (corn stover) incubated in the water column of Great Boiling Spring, NV. Strain JKG1T had cells of a diameter of 0.7 - 0.9 μm and length of ~2.0 μm that formed non-branched multicellular filaments reaching >300 μm. Spores were not formed and dense liquid cultures were red. The temperature range for growth was 45-65 °C, with an optimum of 55 °C. The pH range for growth was 5.6-9.0, with an optimum of 7.5. JKG1T grew as an aerobic heterotroph, utilizing glucose, sucrose, xylose, arabinose, cellobiose, carboxymethylcellulose, filter paper, microcrystalline cellulose, xylan, starch, casamino acids, tryptone, peptone, yeast extract, acetate, citrate, lactate, pyruvate, and glycerol as sole carbon sources, and was not observed to photosynthesize. The cells stained Gram-negative. Phylogenetic analysis using 16S rRNA gene sequences placed the new isolates in the class Chloroflexia, but distant from other cultivated members, with the highest sequence identity of 82.5% to Roseiflexus castenholzii. The major quinone was menaquinone-9; no ubiquinones were detected. The major cellular fatty acids (>5%) were C18:0, anteiso-C17:0, iso-C18:0, and iso-C17:0. C16:0, iso-C16:0, and C17:0. The peptidoglycan amino acids were alanine, ornithine, glutamic acid, serine, and asparagine. Whole-cell sugars included mannose, rhamnose, glucose, galactose, ribose, arabinose, and xylose. Morphological, phylogenetic, and chemotaxonomic results suggest that JKG1T is representative of a new lineage within the class Chloroflexia, which we propose to designate Kallotenue papyrolyticum gen. nov., sp. nov., Kallotenuaceae fam. nov., Kallotenuales ord. nov.

  9. Thermococcus sulfurophilus sp. nov., a New Hyperthermophilic, Sulfur-Reducing Archaeon Isolated from Deep-Sea Hydrothermal Vent

    Science.gov (United States)

    Pikuta, Elena V.; Hoover, Richard B.; Whitman, William B.; Marsic, Damien; Garriott, Owen; Six, N. Frank (Technical Monitor)

    2002-01-01

    A new hyperthermophilic, anaerobic, sulfur-reducing, organo-heterotrophic archaeon, strain OGL-20P, was isolated from "black smoker" chimney material at the Rainbow hydrothermal vent site in the Atlantic Ocean (36.2 N; 33.9 W). The cells of strain OGL-20P have irregular coccoid shape and are motile with a single flagellum. Growth occurs within pH range of 5.5-8.2 (optimal at pH 7.0-7.2), salinity range of 1-5% NaCl (optimal concentration 3% NaCl wt/vol), and temperature range of +55 C to +94 C (optimal growth at +83 C to +85 C). Strain OGL-20P is resistant to freezing (at -20 C). New isolate is strictly anaerobic with sulfur-type of respiration. A limited number of compounds are utilized as electron donors, including peptone, becto-tryptone, casamino-acids, and yeast extract but does not grow with separate amino acids. Sulfur and Iron can be used as electron acceptors; but not sulfate, sulfite, thiosulfate or nitrate. Strain OGL-20P is resistant to chloramphenicol, kanamycin, and gentamycin. Growth of str. OGL20P is inhibited by tetracyclin but not by Na2MoO4. The G+C content of DNA is 57.2 mol%. The 16S ribosomal RNA sequence analysis allows one to classify strain OGL-20P as a representative of a now species of Thermococcus genus. The name Thermococcus sulfurophilus op. nov., was suggested for the new isolate, type strain OGL-20P (sup T) (= ATCC BAA_394 (sup T) = DSM...(supT)).

  10. Thermococcus Thioreducens sp. Nov., a Novel Hyperthermophilic, Obligately Sulfur-reducing Archaeon from a Deep-sea Hydrothermal Vent

    Science.gov (United States)

    Pikuta, Elena V.; Marsic, Damien; Itoh, Takashi; Bej, Asim K.; Tang, Jane; Whitman, William B.; Ng, Joseph D.; Garriott, Owen K.; Hoover, Richard B.

    2007-01-01

    A hyperthermophilic, sulfur-reducing, organo-heterotrophic archaeon, strain OGL-20P was isolated from black smoker chimney material from the Rainbow hydrothermal vent site on the Mid-Atlantic Ridge (36.2 N, 33.9 W). The cells of strain OGL-20P(sup T) have an irregular coccoid shape and are motile with a single flagellum. Growth was observed within the pH range 5.0-8.5 (optimum pH 7.0), NaCl concentration range 1-5 % (w/v) (optimum 3%), and temperature range 55-94 C (optimum 83-85 C). The novel isolate is strictly anaerobic and obligately dependent upon elemental sulfur as an electron acceptor, but it does not reduce sulfate, sulfite, thiosulfate, iron (III) or nitrate. Proteolysis products (peptone, bacto-tryptone, casamino-acids, and yeast extract) are utilized as substrates during sulfur-reduction. Strain OGL-20P(sup T) is resistant to ampicillin, chloramphenicol, kanamycin, and gentamycin, but sensitive to tetracycline and rifampicin. The G+C content of DNA is 52.9 mol%. The 16S rRNA gene sequence analysis revealed that strain OGL-20P(sup T) is closely related to Thermococcus coalescens and related species, but no significant homology by DNA-DNA hybridization was observed between those species and the new isolate. On the basis of physiological and molecular properties of the new isolate, we conclude that strain OGL-20P(sup T) represents a new separate species within the genus Thermococcus, and propose the name Thermococcus thioreducens sp. nov. The type strain is OGL-20P(sup T) (= ATCC BAA-394(sup T) = JCM 12859(sup T) = DSM 14981(sup T)).

  11. Crassaminicella profunda gen. nov., sp. nov., an anaerobic marine bacterium isolated from deep-sea sediments.

    Science.gov (United States)

    Lakhal, Raja; Pradel, Nathalie; Postec, Anne; Ollivier, Bernard; Cayol, Jean-Luc; Godfroy, Anne; Fardeau, Marie-Laure; Galés, Grégoire

    2015-09-01

    A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766H(T), was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002  m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766H(T) grew at temperatures of 25-45 °C (optimum 30 °C), pH 6.7-8.1 (optimum 7.5) and in a salinity of 5-60 g l(-1) NaCl (optimum 30 g l(-1)). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H2 and CO2. Sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, nitrate, nitrite and Fe(III) were not used as terminal electron acceptors. The predominant cellular fatty acids were C14  : 0, C16 : 1ω7, C16 : 1ω7 DMA and C16 : 0. The main polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipids. The G+C content of the genomic DNA was 33.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Ra1766H(T) was affiliated to cluster XI of the order Clostridiales, phylum Firmicutes. The closest phylogenetic relative of Ra1766H(T) was Geosporobacter subterraneus (94.2% 16S rRNA gene sequence similarity). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766H(T) ( = DSM 27501(T) = JCM 19377(T)) is proposed to be the type strain of a novel species of a novel genus, named Crassaminicella profunda.

  12. Growth of the facultative anaerobe Shewanella putrefaciens by elemental sulfur reduction

    Science.gov (United States)

    Moser, D. P.; Nealson, K. H.

    1996-01-01

    The growth of bacteria by dissimilatory elemental sulfur reduction is generally associated with obligate anaerobes and thermophiles in particular. Here we describe the sulfur-dependent growth of the facultatively anaerobic mesophile Shewanella putrefaciens. Six of nine representative S. putrefaciens isolates from a variety of environments proved able to grow by sulfur reduction, and strain MR-1 was chosen for further study. Growth was monitored in a minimal medium (usually with 0.05% Casamino Acids added as a growth stimulant) containing 30 mM lactate and limiting concentrations of elemental sulfur. When mechanisms were provided for the removal of the metabolic end product, H2S, measurable growth was obtained at sulfur concentrations of from 2 to 30 mM. Initial doubling times were ca. 1.5 h and substrate independent over the range of sulfur concentrations tested. In the cultures with the highest sulfur concentrations, cell numbers increased by greater than 400-fold after 48 h, reaching a maximum density of 6.8 x 10(8) cells ml-1. Yields were determined as total cell carbon and ranged from 1.7 to 5.9 g of C mol of S(0) consumed-1 in the presence of the amino acid supplement and from 0.9 to 3.4 g of C mol of S(0-1) in its absence. Several lines of evidence indicate that cell-to-sulfur contact is not required for growth. Approaches for the culture of sulfur-metabolizing bacteria and potential ecological implications of sulfur reduction in Shewanella-like heterotrophs are discussed.

  13. Palaeococcus pacificus sp. nov., an archaeon from deep-sea hydrothermal sediment.

    Science.gov (United States)

    Zeng, Xiang; Zhang, Xiaobo; Jiang, Lijing; Alain, Karine; Jebbar, Mohamed; Shao, Zongze

    2013-06-01

    A hyperthermophilic, anaerobic, piezophilic archaeon (strain DY20341(T)) was isolated from a sediment sample collected from an East Pacific Ocean hydrothermal field (1° 37' S 102° 45' W) at a depth of 2737 m. The cells were irregular cocci, 0.8-1.5 µm in diameter. Growth was observed between 50 and 90 °C (optimum 80 °C), pH 5.0 and 8.0 (optimum pH 7.0), 1% and 7% (w/v) sea salts (Sigma, optimum 3%), 1% and 4% (w/v) NaCl (optimum 3%) and 0.1 and 80 MPa (optimum 30 MPa). The minimum doubling time was 66 min at 30 MPa and 80 °C. The isolate was an obligate chemoorganoheterotroph, capable of utilizing complex organic compounds and organic acids including yeast extract, peptone, tryptone, casein, starch, Casamino acids, citrate, lactate, acetate, fumarate, propanoate and pyruvate for growth. It was strictly anaerobic and facultatively dependent on elemental sulfur or sulfate as electron acceptors, but did not reduce sulfite, thiosulfate, Fe(III) or nitrate. The presence of elemental sulfur enhanced growth. The G+C content of the genomic DNA was 43.6 ± 1 mol%. 16S rRNA gene sequence analysis revealed that the closest relative of the isolated organism was Palaeococcus ferrophilus DMJ(T) (95.7% 16S rRNA gene similarity). On the basis of its physiological properties and phylogenetic analyses, the isolate is considered to represent a novel species, for which the name Palaeococcus pacificus sp. nov. is proposed. The type strain is strain DY20341(T) (=JCM 17873(T)=DSM 24777(T)).

  14. Evaluation of supplementation of sucrose medium on the synthesis of Zymomonas mobilis bio-products - doi: 10.4025/actascibiolsci.v32i3.1519

    Directory of Open Access Journals (Sweden)

    Maria Antonia Pedrini Colabone Celligoi

    2010-09-01

    Full Text Available The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.The effect of the variables pantothenic acid, yeast extract and sodium chloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell

  15. PERBAIKAN PROSES FERMENTASI BIJI KAKAO KERING DENGAN PENAMBAHAN TETES TEBU, KHAMIR, DAN BAKTERI ASAM ASETAT

    Directory of Open Access Journals (Sweden)

    Donny Widianto, Ajeng Dara Pramita, dan Sri Wedhastri

    2013-12-01

    Full Text Available Most of cocoa beans produced by smallholder farmers were non fermented which can be improved by modifiedfermentation processing. This study was aimed to inverstigate the influence of molasses, yeast Saccharomycescerevisiae, and Acetobacter aceti addition on dried cocoa beans fermentation process.Fresh cocoa beans were dried in a glasshouse and its reducing sugar was analyzed before and after drying. Asmall plastic bucket (20 cm diameter and 30 cm height with aeration holes was used as fermentation vessel. Driedcocoa beans were soaked in distilled water for 4 hours, inoculated with yeast and acetic acid bacteria cultures, andmolasses were added at two different concentration, i.e, 1 and 1.5 times of reducing sugar lost during drying.Reducing sugar, ethanol, titrated acid, population of yeast, and acetic acid bacteria were monitored duringfermentation. After fermentation the beans were sun dried and its pH and degree of fermentation were determinedto assess the bean quality.The results showed that the addition of molasses mostly at the level of 1.5, S. cerevisiae, and A. aceti increasereducing sugar, ethanol, titrated acid, yeast and acetic acid bacteria of fermentation liquid (pulp. The highestpercentage of fermented beans (68.4 % was achieved by addition of S. cerevisiae, A. aceti, and molasses atthe level 1.5. It is likely that the addition of S. cerevisiae, A. aceti, and molasses could improve fermentationprocessing of dried cocoa bean.

  16. Controlling Lipid Fluxes at Glycerol-3-phosphate Acyltransferase Step in Yeast

    Science.gov (United States)

    Marr, Nancy; Foglia, Julena; Terebiznik, Mauricio; Athenstaedt, Karin; Zaremberg, Vanina

    2012-01-01

    The ability to channel excess fatty acids into neutral lipids like triacylglycerol (TAG) is a critical strategy used by cells to maintain lipid homeostasis. Upon activation to acyl-CoA, fatty acids become readily available as substrates for acyltransferases involved in neutral lipid synthesis. Neutral lipids are then packed into organelles derived from the endoplasmic reticulum called lipid particles (LPs). The first acylation step in the de novo pathway for TAG synthesis is catalyzed by glycerol-3-phosphate acyltransferases (GPATs). Two isoforms, Gat1p/Gpt2p and Gat2p/Sct1p, are present in the yeast Saccharomyces cerevisiae. Previous evidence indicated that these enzymes contribute differentially to the synthesis of TAG in actively growing cells. In this work we studied the role of the yeast GPATs in the formation of LPs induced by a surplus of oleic acid. Yeast lacking Gat1p (but not Gat2p) were sensitive to oleate and failed to accumulate LPs induced by this unsaturated fatty acid. It is shown that oleate induces dephosphorylation of Gat1p as well as an increment in its levels. Most importantly, we identified novel Gat1p crescent structures that are formed in the presence of oleate. These structures are connected with the endoplasmic reticulum and are intimately associated with LPs. No such structures were observed for Gat2p. A crucial point of control of lipid fluxes at the GPAT step is proposed. PMID:22267742

  17. Yeast prions: structure, biology, and prion-handling systems.

    Science.gov (United States)

    Wickner, Reed B; Shewmaker, Frank P; Bateman, David A; Edskes, Herman K; Gorkovskiy, Anton; Dayani, Yaron; Bezsonov, Evgeny E

    2015-03-01

    A prion is an infectious protein horizontally transmitting a disease or trait without a required nucleic acid. Yeast and fungal prions are nonchromosomal genes composed of protein, generally an altered form of a protein that catalyzes the same alteration of the protein. Yeast prions are thus transmitted both vertically (as genes composed of protein) and horizontally (as infectious proteins, or prions). Formation of amyloids (linear ordered β-sheet-rich protein aggregates with β-strands perpendicular to the long axis of the filament) underlies most yeast and fungal prions, and a single prion protein can have any of several distinct self-propagating amyloid forms with different biological properties (prion variants). Here we review the mechanism of faithful templating of protein conformation, the biological roles of these prions, and their interactions with cellular chaperones, the Btn2 and Cur1 aggregate-handling systems, and other cellular factors governing prion generation and propagation. Human amyloidoses include the PrP-based prion conditions and many other, more common amyloid-based diseases, several of which show prion-like features. Yeast prions increasingly are serving as models for the understanding and treatment of many mammalian amyloidoses. Patients with different clinical pictures of the same amyloidosis may be the equivalent of yeasts with different prion variants. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  18. Specific phenolic compounds and sensory properties of a new dealcoholized red wine with pomegranate (Punica granatum L.) extract.

    Science.gov (United States)

    Tárrega, Maria Amparo; Varela, Paula; Fromentin, Emilie; Feuillère, Nicolas; Issaly, Nicolas; Roller, Marc; Sanz-Buenhombre, Marisa; Villanueva, Sonia; Moro, Carlos; Guadarrama, Alberto; Fiszman, Susana

    2014-09-01

    The pomegranate (Punica granatum L.) fruit has a long history of human consumption and possesses notable antioxidant and cardiovascular properties. This work evaluated the feasibility to provide a new functional beverage based on a dealcoholized red wine matrix supplemented by a pomegranate extract. The potential bioactive compounds in the pomegranate extract, punicalagin A and B and ellagic acid, were analyzed during the downstream process in order to evaluate the functional dose in the final beverage. The addition of pomegranate extract to the dealcoholized red wine resulted in a product with more intense yeast odor, acidity, yeast flavor, and astringency and with a less intense berry flavor. Consumer acceptance of the product was also investigated and the results revealed the existence of a niche of consumers willing to consume dealcoholized wine enriched with pomegranate extract. After tasting, 50% and 40% of those consumers initially interested by this product concept declared to be interested to purchase the control sample and the functional beverage, respectively. The daily consumption of two servings of 250 mL of this new pomegranate-enriched dealcoholized wine provides 82 mg of total ellagitannins, corresponding to the sum of punicalagin A and B and ellagic acid. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  19. Evaluation of EXPLOSAFE. Explosion Suppression System for Aircraft Fuel Tank Protection

    Science.gov (United States)

    1980-07-01

    drain, reducing to 0.52 milligrams per gallon for the second drain. The residue collected consisted of dirt, fiber , and aluminum dust. The data for...in in u) Lo in in in in -v " ’ v - ) m mI r m 0’.i 0 0 m1 aaa 001 COCO N- - -" 10 10 10 lo in in in -w’ M&#34 m’ U) .4 N N M&#34 in N m1 -4 C" n N- 01 r-4 m...heavily con- taminated for a particle size and count analysis to be performed. (4) Due to agglomeration of fibers and silicous particles on samples

  20. Environmental Durability of Adhesively Bonded Joints

    Science.gov (United States)

    1997-10-14

    REPORT DOCUMENTATION PAGE Form Approved OMB No. 07040188 Public reporting burden for tis collection of information isestimated to average 1 hour per...transmittance and reflectance spectroscopy was performed using Nicolet’s OMNIC software for the set-up, control, and analysis of spectroscopic scans... publications inciudc: JAhIN’. W S. iind Butjkus, L.M., &#34Considetring E~nvironmental Condjitions ill the Design ()’ Bonded Structures: A Fracture

  1. Special Course on V/STOL Aerodynamics

    Science.gov (United States)

    1984-04-01

    Predict CLMAX of Arbitrary Airfoil Sections", AIAA Paper 78-156, January, 1978. 27. LeBalleur, J. C., and Neron , M., &#34Cacul D’Ecoulements Visqueux...exchange test sections, with options such as those shown in Fig. 16(b). For complicated powered models with a network of instrumentation needed, this makes...both three inter- nal balances and 200 surface-pressure taps, which resulted in the tubing network (Fig. 91) leading to the on-board Scanivalves. The

  2. Field performance of timber bridges. 13, Mohawk Canal stress-laminated bridge

    Science.gov (United States)

    P. D. Hilbrich Lee; X. Lauderdale

    The Mohawk Canal bridge was constructed in August 1994, just outside Roll, Arizona. It is a simple-span, double-lane, stress-laminated deck superstructure, approximately 6.4 m (21 ft) long and 10.4 m (34 ft) wide and constructed with Combination 16F-V3 Douglas Fir glued-laminated timber beam laminations. The performance of the bridge was monitored continuously for 2...

  3. High Angle of Attack Aerodynamics

    Science.gov (United States)

    1979-01-01

    strakes were added promises well for the flight tests. At the time of writing the test aircraft had just made its first flight. A photograph taken from...I- : : I 7(71000 MUS I I- UN ILIA I I GAS 1.~W 74LL GOR I L7AP7 07000 Fig.VAIO 11M&#34 ORBITERt CEAP$I’ WN TUNNE MEGR~ Fig." 1LS SV2MDA. IELT

  4. Three-Dimensional Interactions and Vortical Flows with Emphasis on High Speeds

    Science.gov (United States)

    1980-07-01

    pylons, boundary-layer diverters, and unfaired junctions of the wing and tail surfaces with the body are important examples (Meyer 1968; Peake, Rainbird...209, Jan. 1975. Fiechter, M., &#34 Uber Wirbelsysteme an schlanken Rotationskbrpern und ihren Einfluss auf die aerodynamischen Beiwerte," Deutsch...10 0 O . ’ 1N . 2 .4 . .1LL 61 SHUTTLE WINDWARD SURFACE H4EATING FOR (d) CALCULATED WINDWARD VPAN*=S WIND- TUNNEL CONOITIONS M_ - 7.4% 1 EATING FOR

  5. USSR Report, Sociological Studies, Number 4, Oct-Nov-Dec 1983

    Science.gov (United States)

    1984-03-06

    34 [ Essays on the Logic of Historical Research] (1961), "Mneniya o mire i mir mneniy" [Opinions About the World and the World of Opinions] (1967...Journeys: Essays 1960-1980," London, 1980, p 149. 10. Novak, M., &#34The American Vision: An Essay on the Future of Democratic Capitalism," Washington, 1979...garian, Progress, Moscow, 1982, 384 pages] [Text] If people say that love is an eternal theme, they usually have in mind lyrical fiction, and not

  6. Combustor Modelling

    Science.gov (United States)

    1980-02-01

    TABLE B I •~ 0 ~ 0 ,U6- v z VC r’𔃻/r 4!𔃻 !.!t + Ga/ fta clippe ditiuin cin nu’fI’ I Q- 1. C1, 1-++.9?Q0 tC The Gaussian clipped p.d~f...Film Cooling Geometries of a Practical Nature", Cranfield Int’II Prop. Symposium, 1969. 40. Papell , S.S. & Trout, A.M., &#34Experimental Investigation of

  7. Bioassessment of the Standard Elutriate Test

    Science.gov (United States)

    1976-09-01

    that the growth of the green algal, Chlorella vulgaris , was reduced approximately 50 percent in the presence of 2.0 ppm zinc. Payne (12) reported that...Environmental Protection Agency, Washington, DC, Oct., 1973. 14. Rachlin, Y. W., and Farran, M., &#34Growth Response of the Green Algae Chlorella ... vulgaris to Selective Concentrations of Zinc," Water Research, Vol. 8, 1974, pp. 575-577. 1:). Standard Methods for the Examination of Water and Wastewater

  8. Evaluation of Energy-Sink Stability Criteria for Dual-Spin Spacecraft

    Science.gov (United States)

    1994-06-01

    Unstable Expunental Modelo 14M -- &#34𔃺 a*&90 12W0--mdldn~nw&(~r)s 7Ŕ e low 60 Boo -so 600_ ____ 40. / ___ ____ 30~ 200 - oddpafr o nry-10 0 1 - 10 0 1000...34nbad"): move(980, 47): printt("nok"); move (980.67): priatI ("hount"): 131 sievef%~ -,80 4.7 PC -ft (󈨄 -. * osI nn ftO- move (960. 157): pile

  9. Project Manager’s Guide to the Scientific and Technical Information (STINFO) Program and Technical Publications Process

    Science.gov (United States)

    1993-12-01

    Iaporta .. y be definitive for the tubjoct proaentod, exploratory in natura, or an evaluation of critical Aubayato• or of technical problema , 4...International Security 9 Social and Natural Science Studies Field 41 Edit: (Type 3) -Entry of an invalid code when Performance Type is "C" or "M&#34 will...analysis SF Foreign area social science research SP Foreign area policy planAing research BF Identifies databases with data on foreign forces or

  10. Defying Predictions? Chilean Civil-Military Relations Since 1990

    Science.gov (United States)

    2010-06-01

    Pública, (Santiago: Biblioteca del Congreso Nacional, undated). 98 Loveman, For la Patria, 231. 41...Luis Riffo M., &#34Pasado, presente y futuro de la Ley Reservada del Cobre," Biblioteca del Congreso Nacional de Chile, September 23, 2009, http...FRIDE, April 2007. Historia de la Constitución Política, Artículo 101: Fuerzas Armadas, de Orden y Seguridad Pública. Santiago: Biblioteca del

  11. 2ND EF Conference in Turbulent Heat Transfer, Manchester, UK 1998. Volume 2

    Science.gov (United States)

    1998-06-01

    out most of the turbulent transport processes in the flow are being simulated with some realism ; but an LES will often be considerably more...sensitivity [volts/K] ß-aA err) =±(A2+B2U°)E- 1 u X thermal conductivity [W-m^.Kŕ] V cinematic viscosity [m^sŕ] P density [Kg.m&#34 3] <f> energy

  12. How Do We Turn This Thing Off? A Study to Determine an Approach for Making Curtailment of Service and Closure Decisions in Health Care Facilities.

    Science.gov (United States)

    1979-04-09

    22a. NAME OF RESPONSIBLE INDIVIDUAL 22b TELEPHONE (Include Area Code) 22c. OFFICE SYMBOL Lawrence M. Leahy, MAJ, MS (512) 221-6345 HSHA-IHC DO Form...Facilities. Germantown, MD.: Aspen Systems Corporation, 1976. Bierman, Harold Jr., Charles R. Bonini and Warren H. Hausman . Quantitative Analysis for Business...Assessment," Topics in Health Care Financing II, No. 4 ( Summer , 1976), 109-121. Drake, David F. and Kozak, David M. &#34A Primer on Antitrust and Hospital

  13. Next-generation Surface Enhanced Raman Scattering (SERS) Substrates for Hazard Detection

    Science.gov (United States)

    2012-09-01

    substrates at differing concentrations ranging from 0.05 ug/cm2 to 50 ug/cm2. These concentrations were independently validated by UV -Vis...standard Klarite substrates at a concentration of 5.0 ug/cm2. The concentration jetted was validated using a secondary UV -Vis technique.(20, 81) After... Spectros ., 994 (1997). [58] Alexander, T.A., Le, D.M., &#34Characterization of a commercialized SERS-active substrate and its application to the

  14. A Comparative Analysis of Army Physical Readiness Test Results of AMEDD Units Without Formal Physical Training Programs

    Science.gov (United States)

    1984-06-01

    Paul C.; Albrink , Margaret J.; and Krall, John M. &#34Exercise Effects on Fitness,, Lipids, Glucose Tolerance and Insulin Levels in Young Adults...1982): 14-15. Shepherd, Roy J.; Corey, Paul; Renzland, Peter; and Cox Michael . "The Impact of Changes in Fitness and Lifestyle Upon Health Care...Utilization." Canadian Journal of Public Health 74 (January/February 1983): 51-55. Shepherd, Roy J.; Corey, Paul; Renzland, Peter; and Cox, Michael . "The

  15. Multivariate Modelling of the Career Intent of Air Force Personnel.

    Science.gov (United States)

    1980-09-01

    index (HOPP) was used as a measure of current job satisfaction . As with the Vroom and Fishbein/Graen models, two separate validations were accom...34 Organizational Behavior and Human Performance , 23: 251-267, 1979. Lewis, Logan M. &#34Expectancy Theory as a Predictive Model of Career Intent, Job Satisfaction ...W. Albright. "Expectancy Theory Predictions of the Satisfaction , Effort, Performance , and Retention of Naval Aviation Officers," Organizational

  16. An Investigation of the Factors which Affect the Career Selection Process of Air Force Systems Command Company Grade Officers.

    Science.gov (United States)

    1979-12-01

    faction, occupational preference, or the desirability of good performance . Proposition 2, as formulated by Vroom , predicts the force to act in a...Human Performance , 9: 482-503 (1973). Lewis, Logan M. &#34Expectancy Theory as a Predictive Model of Career Intent, Job Satisfaction , and Institution... Satisfaction , Effort, Performance , and Retention of Naval Aviation Officers," Organizational Behavior and Human Performance , 8: 1-20 (1972). 102 and Lee Roy

  17. Combustion and Mixing Studies in Compressible Flows.

    Science.gov (United States)

    1996-09-01

    Astronautics 2 FULLER ET AL. dence times. It is a primary concern in hypersonic aircraft In fact, studies conducted by Povinelli et al.1 3 and Schetz...downstream. It was reasoned that pressure gradients in the swirling flow. Povinelli et al." such behavior should lead to increased turbulence levels...E.M., &#34Design and Calibration of Stagnation Tem- tion, 1968, pp. 1153-1162.11 .perature Probes for Use at High Supersonic Speeds and Elevated Povinelli

  18. Exploitation of Smart Materials and Sensors as Disruptive Technologies

    Science.gov (United States)

    2010-03-01

    could form part of an automated flight control system that might minimise buffeting or incipient stall. It might be used on manned aircraft to...healing agents into a material system on its original performance must be accounted for. Self-healing by micro-encapsulation has been used to...Massachusetts, USA(2006). 135 Afshar V, S., Warren -Smith, S. C., & Monro, T. M., &#34Enhancement of Fluorescence-based Sensing Using Microstructured

  19. Factors Affecting the Performance of Hispanic and Non-Hispanic Marine Corps Enlistees

    Science.gov (United States)

    2015-03-01

    NHSDG Non-High School Diploma Graduates OccFld Occupational Field OMB Office of Management and Budget OLS Ordinary Least Squares PC ASVAB Paragraph...and the te1m &#34Hispanic" be replaced by "Hispanic or Latino " (Federal Register, 1997). Although this changed how data was collected and stored, the...U.S. Census Bureau still uses the 1977 OMB definition of Hispanic or Latino (Humes et al., 2011). B. REPRESENTATION As indicated in Table 1, Panel 1

  20. Chinese-English Electronics and Telecommunications Dictionary. Volume 1

    Science.gov (United States)

    1976-06-01

    alang ) 14 baldong tianxian «#x« swinging antenna 15 baldong xiaoying tf # 4k tt hunt effect 16 baidongfa m^& &#34awing" method 17 baidong-ahl tianxian ma...pottlneaa09 apot meaaurenent 10 "spot wobbler" generator ( alang ) 11 apot error Indicator 12 bomlte 13 atreak; »tripe 14 remover IS tranaportatlon...shengzukcnglyu faxlangll Fayang welyllyu faze faal tanhuang faxian f alang fanyl fanzhuan fanzhuan dlanya fanzhuan kalguan fanllshui

  1. Thermophysical Properties of Matter - the TPRC Data Series. Volume 13. Thermal Expansion - Nonmetallic Solids

    Science.gov (United States)

    1977-01-01

    topography of the state of knowledge on the thermal expansion of nonmetallic solids. We believe there is also much food for reflec- West Lafayette...34 Lithium Silicates ......... 713 209 Magnesium Metasilicate MgSiO. .. ......... 715 210 Magnesium Orthosilicate Mg2 SiO . . . . . . . . . . . . 718 211...Antiferromagnetism of Praseodymium," Phys. Rev. Letters, 12(20), 553-5, 1964. 66. Goode, J.M., &#34Phase Transition Temperature of Polonium ,"J. Chem. Phys., 26(5), 1269

  2. Playing Detective: Reconstructing Software Architecture from Available Evidence

    Science.gov (United States)

    1997-10-01

    information • PostgreSQL (based on POSTGRES [Stonebraker 90]) for model storage • IAPR [Kazman 96c], RMTool [Murphy 95], and Perl for analysis and...720-741. Stonebraker, M.; Rowe, L; & Hirohama, M. ’The Implementation of POSTGRES ." IEEE Transactions on Knowledge and Data Engineering 2,1 (March...Engineering 19,7 (July 1993): 720-741. Stonebraker, M.; Rowe, L; & Hirohama, M. &#34The Implementation of POSTGRES ." IEEE Transactions on Knowledge and

  3. The Zapatista Social Netwar in Mexico

    Science.gov (United States)

    1998-01-01

    on the Internet. 4From a statement by Subcommandante Marcos, March 4, 1994, as reported by the Academia Mexicana de Derechos Humanos , Special...Indigenas de Mexico, Mexico City: Comisiön Nacional de Derechos Humanos , 1995. Evan, William M., &#34An Organization-Set Model of Interorganizational...City: Comision Nacional de Dere- chos Humanos , 1995. Meisel, James, The Fall of the Republic: Military Revolt in France, Ann Arbor: University of

  4. A State-of-the-Art Assessment of Automatic Name Placement.

    Science.gov (United States)

    1986-08-01

    processing and the annotation problem," Proc. 3rd Scandinavian Conference on Image Analyms, Chartwell-Bratt Ltd., Copenhagen, Denmark, July 1983. This paper...develop an automatic name placement system. 11 Balodis, M., &#34Positioning of typography on maps," Proc. ACSM Pall Con- vention, Salt Lake City, Utah, Sept...1983, pp. 28-44. This article deals with the selection of typography for maps. It describes psycho-visual experiments with groups of individuals to

  5. Experiments, Theory, and Simulation on the Evolution of Fabric in Granular Materials

    Science.gov (United States)

    1992-07-27

    Deformation," Intl. J. Plast., 7, 141-160, 1991. [4] Arminjon, M. &#34Sur le Champ de Rotation des Cristaux dans un Poly- cristal Dform6 Plastiquement ...in Metals," J. Inst. Mftals, 62, 307, 1938. [17] Zbib, H. M. and Aifantis, E. C. --On the Concept of Relative Spin and its Implications to Large... Concept of Relative Spin and its Implications to Large Deformation Theories. Part I: Anisotropic Hard- ening Plasticity," Acta Mechanica, 74, 35, 1988b. 107

  6. Computerized Buckling Analysis of Shells

    Science.gov (United States)

    1981-06-01

    Simple Examples to Illu-trate Various Types of Buckling Column Buckling In order to make the discussion of the basic concepts introduced in connec...the optimum design of a square box column obtained from an "* analysis in which the effective width concept is used and collapse is assumed to occur...nology, Delft., pp 335-344 (1969). 120 Save, M., &#34Verification experimentale de l’analyse limite plastique des plaques et des coques en acier doux

  7. Application of an Operational Audit Model in a Not For Profit Hospital

    Science.gov (United States)

    1993-12-01

    Advisor David R. Whipple, Chairman Department of Administrative Sciences ii AIITRCT This thesis has examined various problems facing the not- for...the interviewee and more closely gauge the validity of the comments. ( Fetterman , 1986) As the auditor sifts through bits and pieces of conversations... Fetterman , D.M., &#34Operational Auditing: A Cultural Approach," The Internal Auditor, April 1986, pp. 48-54. Fisher, J., How to Manage a Nonprofit

  8. Rochester Connectionist Papers. 1979-1985

    Science.gov (United States)

    1985-12-01

    updated and improved version of the thesis account of recent neurolinguistic data. Fanty, M., &#34Context-free parsing in connectionist networks." TR 174...April 1982. Our first large program in the connectionist paradigm. It simulates a multi- layer network for recognizing line drawings of Origami figures...The program successfully deals with noise and simple occlusion and the thesis incorporates many key ideas on designing and running large models. Small

  9. Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

    Science.gov (United States)

    Moreno, Luisa Z; Miraglia, Fabiana; Loureiro, Ana P; Kremer, Frederico S; Eslabao, Marcus R; Dellagostin, Odir A; Lilenbaum, Walter; Vasconcellos, Silvio A; Heinemann, Marcos B; Moreno, Andrea M

    2018-01-01

    Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species. PMID:29538491

  10. Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain.

    Science.gov (United States)

    Moreno, Luisa Z; Miraglia, Fabiana; Loureiro, Ana P; Kremer, Frederico S; Eslabao, Marcus R; Dellagostin, Odir A; Lilenbaum, Walter; Vasconcellos, Silvio A; Heinemann, Marcos B; Moreno, Andrea M

    2018-03-12

    Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.

  11. Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

    Directory of Open Access Journals (Sweden)

    Luisa Z Moreno

    2018-03-01

    Full Text Available Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.

  12. Sporosalibacterium faouarense gen. nov., sp. nov., a moderately halophilic bacterium isolated from oil-contaminated soil.

    Science.gov (United States)

    Rezgui, Raja; Ben Ali Gam, Zouhaier; Ben Hamed, Said; Fardeau, Marie-Laure; Cayol, Jean-Luc; Maaroufi, Abderrazak; Labat, Marc

    2011-01-01

    A novel strictly anaerobic, moderately halophilic and mesophilic bacterium, designated strain SOL3f37(T), was isolated from a hydrocarbon-polluted soil surrounding a deep petroleum environment located in south Tunisia. Cells of strain SOL3f37(T) stained Gram-positive and were motile, straight and spore-forming. Strain SOL3f37(T) had a typical Gram-positive-type cell-wall structure, unlike the thick, multilayered cell wall of its closest relative Clostridiisalibacter paucivorans. The major fatty acids were iso-C(15 : 0) (41 %), iso-C(14 : 0) 3-OH and/or iso-C(15 : 0) dimethyl acetal (21.6 %), iso-C(13 : 0) (4.4 %), anteiso-C(15 : 0) (3.9 %) and iso-C(15 : 1) (2.8 %). Strain SOL3f37(T) grew between 20 and 48 °C (optimum 40 °C) and at pH 6.2-8.1 (optimum pH 6.9). Strain SOL3f37(T) required at least 0.5 NaCl l(-1) and grew in the presence of NaCl concentrations up to 150 g l(-1) (optimum 40 g l(-1)). Yeast extract (2 g l(-1)) was required for degradation of pyruvate, fumarate, fructose, glucose and mannitol. Also, strain SOL3f37(T) grew heterotrophically on yeast extract, peptone and bio-Trypticase, but was unable to grow on Casamino acids. Sulfate, thiosulfate, sulfite, elemental sulfur, fumarate, nitrate and nitrite were not reduced. The DNA G+C content was 30.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SOL3f37(T) was a member of the family Clostridiaceae in the order Clostridiales; strain SOL3f37(T) was related to members of various genera of the family Clostridiaceae. It exhibited highest 16S rRNA gene sequence similarity (93.4 %) with Clostridiisalibacter paucivorans 37HS60(T), 91.8 % with Thermohalobacter berrensis CTT3(T) and 91.7 % with Caloranaerobacter azorensis MV1087(T). On the basis of genotypic, phenotypic and phylogenetic data, it is suggested that strain SOL3f37(T) represents a novel species in a new genus. The name Sporosalibacterium faouarense gen. nov., sp. nov. is

  13. Coordinated surface activities in Variovorax paradoxus EPS

    Directory of Open Access Journals (Sweden)

    Gregory Glenn A

    2009-06-01

    Full Text Available Abstract Background Variovorax paradoxus is an aerobic soil bacterium frequently associated with important biodegradative processes in nature. Our group has cultivated a mucoid strain of Variovorax paradoxus for study as a model of bacterial development and response to environmental conditions. Colonies of this organism vary widely in appearance depending on agar plate type. Results Surface motility was observed on minimal defined agar plates with 0.5% agarose, similar in nature to swarming motility identified in Pseudomonas aeruginosa PAO1. We examined this motility under several culture conditions, including inhibition of flagellar motility using Congo Red. We demonstrated that the presence of a wetting agent, mineral, and nutrient content of the media altered the swarming phenotype. We also demonstrated that the wetting agent reduces the surface tension of the agar. We were able to directly observe the presence of the wetting agent in the presence and absence of Congo Red, and found that incubation in a humidified chamber inhibited the production of wetting agent, and also slowed the progression of the swarming colony. We observed that swarming was related to both carbon and nitrogen sources, as well as mineral salts base. The phosphate concentration of the mineral base was critical for growth and swarming on glucose, but not succinate. Swarming on other carbon sources was generally only observed using M9 salts mineral base. Rapid swarming was observed on malic acid, d-sorbitol, casamino acids, and succinate. Swarming at a lower but still detectable rate was observed on glucose and sucrose, with weak swarming on maltose. Nitrogen source tests using succinate as carbon source demonstrated two distinct forms of swarming, with very different macroscopic swarm characteristics. Rapid swarming was observed when ammonium ion was provided as nitrogen source, as well as when histidine, tryptophan, or glycine was provided. Slower swarming was observed

  14. Functional genes and thermophilic microorganisms responsible for arsenite oxidation from the shallow sediment of an untraversed hot spring outlet.

    Science.gov (United States)

    Yang, Ye; Mu, Yao; Zeng, Xian-Chun; Wu, Weiwei; Yuan, Jie; Liu, Yichen; Guoji, E; Luo, Feng; Chen, Xiaoming; Li, Hao; Wang, Jianing

    2017-05-01

    Hot Springs have unique geochemical features. Microorganisms-mediated arsenite oxidation is one of the major biogeochemical processes occurred in some hot springs. This study aimed to understand the diversities of genes and microorganisms involved in arsenite oxidation from the outlet of an untraversed hot spring located at an altitude of 4226 m. Microcosm assay indicated that the microbial community from the hot spring was able to efficiently oxidize As(III) using glucose, lactic acid, yeast extract or sodium bicarbonate as the sole carbon source. The microbial community contained 7 phyla of microorganisms, of which Proteobacteria and Firmicutes are largely dominant; this composition is unique and differs significantly from those of other described hot springs. Twenty one novel arsenite oxidase genes were identified from the samples, which are affiliated with the arsenite oxidase families of α-Proteobacteria, β-Proteobacteria or Archaea; this highlights the high diversity of the arsenite-oxidizing microorganisms from the hot spring. A cultivable arsenite-oxidizer Chelatococcu sp. GHS311 was also isolated from the sample using enrichment technique. It can completely convert 75.0 mg/L As(III) into As(V) in 18 days at 45 °C. The arsenite oxidase of GHS311 shares the maximal sequence identity (84.7%) to that of Hydrogenophaga sp. CL3, a non-thermotolerant bacterium. At the temperature lower than 30 °C or higher than 65 °C, the growth of this strain was completely inhibited. These data help us to better understand the diversity and functional features of the thermophilic arsenite-oxidizing microorganisms from hot springs.

  15. Biosynthesis of vitamin C by yeast leads to increased stress resistance.

    Directory of Open Access Journals (Sweden)

    Paola Branduardi

    Full Text Available BACKGROUND: In industrial large scale bio-reactions micro-organisms are generally exposed to a variety of environmental stresses, which might be detrimental for growth and productivity. Reactive oxygen species (ROS play a key role among the common stress factors--directly--through incomplete reduction of O(2 during respiration, or indirectly--caused by other stressing factors. Vitamin C or L-ascorbic acid acts as a scavenger of ROS, thereby potentially protecting cells from harmful oxidative products. While most eukaryotes synthesize ascorbic acid, yeast cells produce erythro-ascorbic acid instead. The actual importance of this antioxidant substance for the yeast is still a subject of scientific debate. METHODOLOGY/PRINCIPAL FINDINGS: We set out to enable Saccharomyces cerevisiae cells to produce ascorbic acid intracellularly to protect the cells from detrimental effects of environmental stresses. We report for the first time the biosynthesis of L-ascorbic acid from D-glucose by metabolically engineered yeast cells. The amount of L-ascorbic acid produced leads to an improved robustness of the recombinant cells when they are subjected to stress conditions as often met during industrial fermentations. Not only resistance against oxidative agents as H(2O(2 is increased, but also the tolerance to low pH and weak organic acids at low pH is increased. CONCLUSIONS/SIGNIFICANCE: This platform provides a new tool whose commercial applications may have a substantial impact on bio-industrial production of Vitamin C. Furthermore, we propose S. cerevisiae cells endogenously producing vitamin C as a cellular model to study the genesis/protection of ROS as well as genotoxicity.

  16. Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

    Directory of Open Access Journals (Sweden)

    Jun-Hui Zhang

    Full Text Available To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML in the production of human milk fat substitute (HMFS, we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257Leu were obtained and their hydrolytic and esterification activities were assayed. Using Discovery Studio 3.1 to build models and calculate the binding energy between lipase and substrates, compared to wild-type, the mutant Asp256Ile/His257Leu was found to have significantly lower energy when oleic acid (3.97 KJ/mol decrease and tripalmitin (7.55 KJ/mol decrease were substrates. This result was in accordance with the esterification activity of Asp256Ile/His257Leu (2.37-fold of wild-type. The four mutants were also evaluated for the production of HMFS in organic solvent and in a solvent-free system. Asp256Ile/His257Leu had an oleic acid incorporation of 28.27% for catalyzing tripalmitin and oleic acid, and 53.18% for the reaction of palm oil with oleic acid. The efficiency of Asp256Ile/His257Leu was 1.82-fold and 1.65-fold that of the wild-type enzyme for the two reactions. The oleic acid incorporation of Asp256Ile/His257Leu was similar to commercial Lipozyme RM IM for palm oil acidolysis with oleic acid. Yeast surface-displayed RML mutant Asp256Ile/His257Leu is a potential, economically feasible catalyst for the production of structured lipids.

  17. Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

    Science.gov (United States)

    Zhang, Jun-Hui; Jiang, Yu-Yan; Lin, Ying; Sun, Yu-Fei; Zheng, Sui-Ping; Han, Shuang-Yan

    2013-01-01

    To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257Leu were obtained and their hydrolytic and esterification activities were assayed. Using Discovery Studio 3.1 to build models and calculate the binding energy between lipase and substrates, compared to wild-type, the mutant Asp256Ile/His257Leu was found to have significantly lower energy when oleic acid (3.97 KJ/mol decrease) and tripalmitin (7.55 KJ/mol decrease) were substrates. This result was in accordance with the esterification activity of Asp256Ile/His257Leu (2.37-fold of wild-type). The four mutants were also evaluated for the production of HMFS in organic solvent and in a solvent-free system. Asp256Ile/His257Leu had an oleic acid incorporation of 28.27% for catalyzing tripalmitin and oleic acid, and 53.18% for the reaction of palm oil with oleic acid. The efficiency of Asp256Ile/His257Leu was 1.82-fold and 1.65-fold that of the wild-type enzyme for the two reactions. The oleic acid incorporation of Asp256Ile/His257Leu was similar to commercial Lipozyme RM IM for palm oil acidolysis with oleic acid. Yeast surface-displayed RML mutant Asp256Ile/His257Leu is a potential, economically feasible catalyst for the production of structured lipids.

  18. A Macro Analysis of DoD Logistics Systems. Volume 2. Structure and Analysis of the Air Force Logistics System

    Science.gov (United States)

    1977-09-01

    performance measures discussed earlier is the "Engine Actuarial Data Summary" (EADS) (AFLC Form 992), compiled from D024F actuarial data. EADS is...3.2,452.3 PlyLWn M&#34Is, 404.670 432.603 408,553. 413.940 PMiSMA-00023 AF asm -haiw pua-flywa hma tread =100% PTtU94411 "Weul ’A101) 16,839.6 1.6,308.0...Engine Actuarial Data Summary. ENORS - 2ngine Not Operationally Ready, Supply. EOQ Items - Economic Order Quantity Items; i.e., expense-type items, not

  19. Development of an Automated Gear Tooth Contour Measuring Device.

    Science.gov (United States)

    1982-04-12

    even under the Influence of body beatL " and changng air temperature. Operating the machine in the fluid-filled mode greatly 5-1... the finaL Phase I report incorporating draft corrections. 19. Key WRDS (Coafw so.. me Mo od*f se**p ow somse W, 610 mA..) Mensuration Coordinate...Measuring Machine Noncontact Optic Probe Surface Wear Assessment 2L &911 RACY (Coneaa Me me... odds N msoosw mW SOMWI IV Wle"m &#34 This report covers the

  20. The occurrence of peculiar stars in open clusters

    International Nuclear Information System (INIS)

    Abt, H.A.; Levato, H.

    1978-01-01

    The authors have classified on the MK system a total of 455 stars in 12 open clusters and associations. The classification is based on wide (1.2 mm) spectra of two reciprocal dispersions (39, 128 A mm -1 ) obtained with the Kitt Peak 2.1 m and 90 cm reflectors respectively. The higher dispersion is necessary to show the subtle peculiarities found in some stars. The clusters are the Orion Nebula cluster, Orion OB1 association, Lacerta OB1 association, IC 2602, IC 4665, Pleiades, M39, M34, NGC 2516, NGC 6633, NGC 6475, and Coma. (Auth.)

  1. Proceedings of the Army Numerical Analysis Conference (11th) Held at Frankford Arsenal, Philadelphia, Pa., on 13-14 February 1974

    Science.gov (United States)

    1974-12-01

    World Conference on Earthquake Engineering, Santiago, Chile , January 13 - 18, 1969. 2. English, G. W., and Adams, P. F., "Experiments on Laterally... Chile , January 13 - 18, 1969. 4. Wakabayashi, M., &#34Frames Under Strong Impulsive, Wind or Seismic Loading", Proceedings of the International...while 501 / f(Vzb) = A.. ,. » _i/.. \\i i .. rv»i* e’(x ) + u.[g’(x ) - g’ixj] + vb[h’(xa) - h’(xb)] + V’^ + vIh ’(xb) (uaWV V Lhl

  2. Fresno Air Terminal, California. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F

    Science.gov (United States)

    1981-01-15

    PSYCHROMETRIC SUMMARY ICS S. T.) [ WET SILS TESPERATURE DEPRESSIOS (F) TOTAL I TOTAL , 0 1.2 3-, -6 7-5 𔄃-u a 10 12112 141S. 117igi.l,-20121-.12.222324 l25...VZ T ! ’Nt CA 72 -79 OCT SlAleof STATION UAXJ TEMs M&#34 PAGL 2 18002-2000O New0ti 1.1 T WET BULB TEMPERATURE DEPRESSIO (F) TOTAL TOTAL *(F) 0 1.2 3-4 1

  3. Prediction of Aerodynamic Characteristics of Fighter Wings at High Angles of Attack.

    Science.gov (United States)

    1984-03-01

    potential distribution throughout the network of four points on a body surface great- ly facilitates the flow analysis procedure. Tangential velocity...expensive of computer time. For example, as quoted by McLean, using this coarsest grid network , each 0 surface of the 727-200 wing required 10 minutes of...1980. 19. Le Balleur, J.C. and Neron , M., &#34Calcul D’Ecoulements3 Visqueux Decolles sur Profils D’Ailes par une Approche de Couplage", AGARn CP-291

  4. Matrerials Characterization of Craninal Simulants for Blast Induced Traumatic Brain Injury

    Science.gov (United States)

    2017-08-28

    I . . 1abllfll.1MK:hanit.11Ui¥«Cerir.1tbraum1M1y "’:;: Jj -.,:1 Awp~ ~·-- ... lJl5 ... uuo .. ~ +++ ~::I:: : 1- , "* f ... ~I ::I...coorJl11𔃻NIMl"ll൓.wyt/leniil•,.UOteue11t1m °’’-""’·"-"""o/"’’-’.....,io:-bmM&#34’*~· ~ JJ , f.(~S.(0\\(200$l ~C.VP...-ll-’eH11 1hs tll&utUll .... A!I. tl’ll

  5. AFHRL Conference on Human Appraisal: Proceedings Held at San Antonio, Texas, 19-21 March 1979

    Science.gov (United States)

    1981-06-01

    have branched again into many more specific skills; soldering, writing , hammering, sawing, throwing, arid on and on. These skills and knowledges branch...Berkeley) does--they recommended actually writing critical incidents on the scales, a minimum of two--like the original work with the National League of...asked him "How are you evaluating acceptability in this 128 Wk L7 _ us#m?&#34 And he said to me, "Client satisfaction." I asked him what he ielt , lie said

  6. Event display of a 4-muon candidate in the ATLAS detector

    CERN Multimedia

    ATLAS, Collaboration

    2012-01-01

    Event display of a 4mu candidate. EventNumber: 71902630 RunNumber: 204769 m_4l=125.1 GeV. m_12=86.3 GeV, m_34=31.6 GeV. mu_1: pt=36.1 GeV, eta=1.29, phi=1.33. mu_2: pt=47.5 GeV, eta=0.69, phi=-1.65. mu_3: pt=26.4 GeV, eta=0.47, phi=-2.51. mu_4: pt=71.7 GeV, eta=1.85, phi=1.65.

  7. Enhancement of Combustion and Flame Stabilization Using Transient Non-Equilibrium Plasma

    Science.gov (United States)

    2007-03-31

    Plasma Chemistry, Taormina, Italy, ISPC-564, 22-27 June 2003. 8Ozlem, M.Y., Saveliev A.V., Porshnev, P.I., Fridman, A., Kennedy, L.A., "Non-Equilibrium...Kennedy, L.A., Saveliev , A. and Yardimci, O.M., &#34Gliding Arc Gas Discharge," Progress in Energy and Combustion Science, Vol. 25,1999, pp. 211-231...34Optical Diagnostics of Atmospheric Pressure Air Plasmas,"Plasma Sources Science and Technology, Vol. 12, May 2003, pp. 125-138.31Ozlem, M.Y., Saveliev

  8. Selected Bibliography on Fiber-Reinforced Cement and Concrete. Supplement Number 4.

    Science.gov (United States)

    1982-08-01

    Building Industry," L’Industria Italiana del Cemento , Vol 50, No. 12, Dec 1980, pp 1135-1144. 19. Bartos, P., "Pullout Failure of Fibres Embedded in Cement...Vol 43, No. 11, Nov 1977, pp 561-564. 21. Bassan, M., &#34Model of Behavior of Fiber-Reinforced Concretes Under Impact Stresses," il Cemento , Vol 74, No...Pastes," il Cemento , Vol 75, No. 3, Jul-Sep 1978, pp 277-284. 210. Mills, R. H., "Age-Embrittlement of Glass-Reinforced Concrete Containing Blastfurance

  9. Linear induction motor

    International Nuclear Information System (INIS)

    Barkman, W.E.; Adams, W.Q.; Berrier, B.R.

    1978-01-01

    A linear induction motor has been operated on a test bed with a feedback pulse resolution of 5 nm (0.2 μin). Slewing tests with this slide drive have shown positioning errors less than or equal to 33 nm (1.3 μin) at feedrates between 0 and 25.4 mm/min (0-1 ipm). A 0.86-m (34-in)-stroke linear motor is being investigated, using the SPACO machine as a test bed. Initial results were encouraging, and work is continuing to optimize the servosystem compensation

  10. Fish and Wildlife Habitat Changes Resulting from Construction of a Nine-Foot Channel on Pools 24, 25, and 26 of the Mississippi River and the Lower Illinois River,

    Science.gov (United States)

    1979-04-01

    Sediment, and Water Clarity 132 ii Page Nutrients (Ammonia-nitrogen, Nitrate-nitro- gen, Nitrite -nitrogen, and Total Phosphorus) 135 Heavy Metals and...levels but the supply of suitable mussels had evidently fallen due to over-harvest. The shell processing and button-cutting aspect of the industry...sbos Oma pflm"as pw-a O.s. Ot wp sb1 to a* amem meate . *%fml1e ties, pved tmr r is * psotieftsm OWWOMa twi 14 plow- 1 qut - fas a .6o tow fas O~m &#34a

  11. User Interface Technology Survey.

    Science.gov (United States)

    1987-04-01

    Menu entries may be grouped and orgered Into menu hierarchies. AN levels of a hirarchy may be visible at once, or they may have to be navigated with...s t Xerox aw systemflla3. Ti ft"s lb m&#34k t system leble , ba it ls effective tis of1 iy some User 9101" SWsuc kaybosrdoeute usersome ssteMIS ( Apple Ma...r~stMiTemidndanodApple we tadaml no d Muaioh Isa tadsm macesd to Apple - ,pfr ftc ParSomPT In a beadue-nu of Adobe System lnooporstsd. Scdlbs Is a

  12. John Rodgers WBAS, Honolulu, Hawaii. Revised Uniform Summary of Surface Weather Observations (RUSSWO), Parts A-F

    Science.gov (United States)

    1967-12-05

    4-’Al 0 SM40- INMAN’ - -a-. STATION O: STATION AME: LATITUDE: LONGITUDi. S N MONO STATION HISTORY AND WIND EQUIPMENT INFORMATION DATE OF fYEOF...75) 75,l a 60oo 15.5 16.2 16.2 16.5 16.5 76.5 16.5 16.5 16.5 165 76.o 1os5 16o5 16.5 16.5 16. C $ 000 71 a& Aw -jai -1 k. i, LC6 -m&#34 go, to, IkeA

  13. An Analysis of Department of Defense Financial and Acquisition Policies in Support of Military Contingency Requirements.

    Science.gov (United States)

    1984-03-01

    LEGION 64 (March 1958): 12-13+. 131 Goldich, Robert L. "Mobilization for the Apocalypse." ARMY 28 (May 1978): 22-25+. 132 Gray, Colin S. "The Military...Decerber 8, 1942): 5-7. 632 Drury , V.M. &#34Too Little, Too Late Again (Coordination Detween Armed Services for Peacetirme Planning of Industrial...System." ". Military Review 57 (November, 1977): 76-82. Gray, Colin S. "Military Requirements for U. S. Strategy." MiltarReview 59 (September 1979): 2

  14. Gross resonance-like structure of the complete fusion excitation function for the 16O + 28Si reaction

    International Nuclear Information System (INIS)

    Zheng Jiwen; Zheng Pingzi; Liu Guoxing

    1993-01-01

    The complete fusion excitation function for the 16 O + 28 Si reaction is measured in the incident energy range from 50 to 90 MeV with step of 1.0 MeV using a position sensitive ΔE-E telescope system. The striking gross resonance-like structure is observed when E c.m. c.m. 34.5, 38.5 and 43.0 MeV respectively. The structure vanishes gradually when E c.m. > 46.0 MeV

  15. All Prime Contract Awards by State or Country, Place, and Contractor, FY 85. Part 5 (Arapahoe, Colorado - Washington, D.C.).

    Science.gov (United States)

    1985-01-01

    444444. < MW 0 U 0. U <- OU cI- 0 < :412 8-4 M&#34 I= Ul N >9 ? -4 cc0 a* N - 00 W(0 w.1 co. o U jNNc)InD9- i901I 00 0-4 <-4 Ufl." 20 > -4 ado 30 CI0 1... Porno .....) 4 : W&P 040( 0-I4 4004444 44040000004-44-40.44.44.44-44.44.--4-4.4.4-4.-44-4-4.40.44- I.- do I I a -’ ~ ~ N I , ICPN -440flOo r ntOO40 0-N

  16. Command History. United States Military Assistance Command, Vietnam 1965. Sanitized

    Science.gov (United States)

    1965-01-01

    aspirations of the South Vietnamese people." Although not all Americans chose to recognize this, the doubting minority dwindled numerically, if rot...fforts were being made by VNAF to obtain B-57 Jet aircraft as a substitute for A-l’s in the sixth squadron., (C) In spite of VAF’a jet aspirations the...cargoes. Ti4VC ’AE GRAUUL- t f~illy0)ý m&#34LApO \\ %60150 or𔃾b8" Vaps 6) Cambodian border river surveillance -Waterborne infiltration from Cambodia

  17. United States Air Force Research Initiation Program. 1984 Research Reports. Volume 2.

    Science.gov (United States)

    1986-05-01

    differential determination of adrenaline and noradrenaline in plasma." Clinica Chemica Acta. Vol. 30, pp. 373-376, 1970. 37. Stegemann, J., U. Meier, W...34%" . 1. Agh-anian, G. K. and R. Y. Wang. Physiology and Pharma - coloay cf central serotonergic neurons. In: Psych ph cl . j aGe tion A of P M...34, AIAA Journal, Vol. 18, No. 1, January 1980,"[ ’ "<. .’ip p . 1 1 - 1 5 . 39. Rotta, J.C., "Temperaturverteilungen in der Turbulenten Grenzschicht an

  18. Phased Arrays 1985 Symposium - Proceedings

    Science.gov (United States)

    1985-08-01

    anjl with an1 au~ U lar fy b)eanir. ( mice the 1311 0 ,0 - (a ) ,[ -40.0. -80𔃺 , -90.0 -45.0 0𔃺 45.0 90.0 ANGLE FROM BROADSIDE (DEGREES) aii ia -40,0...Electronic Scanning", RADC-TR-83-128, Dec. 1983. AL) A138808 222 m &#34 ; . . . • " - " - . . . . -" ARRAYS OF COAXIALIY-FED MONOPOLE ELEMENTS IN A PARALLEL...Research Institute Hanscom AFB, MA 01731 Farmingdale, NY 11735 AB ST RAC U Arrays of coaxially-fed monopoles radiating into a parallel plate region

  19. Characterization of Titan III-D Acoustic Pressure Spectra by Least-Squares Fit to Theoretical Model

    Science.gov (United States)

    1980-01-01

    P(f) for a set value of P0 and f0" Mhe inverse transform was taken and the result multiplied by a decaying exponential which modelled the envelope of...0 FORWARD TRANSFORM C IF=1 INVERSE TRANSFORM c C M 0 XREAL AND XIMAG RETURNED AS REAL AND IMAG. FOR FORWARD Xr"RM9; C M= &#34 " " MAGNITUDE AND PHASE...34 .. .. C (PHASE IN DEGREE9) C M=2 XREAL RETURNED AS ’PSD’ XIMAG =0. C HERE ’DSD’ MEANS SUM OF N VALUES OF XREAL = MEAN SQU\\Riz OF INPUT C C FOR INVERSE

  20. Proceedings of the International Conference on the Performance of Off-Road Vehicles and Machines (8th). Volume 3. Held at Cambridge England, on August 5-11, 1984.

    Science.gov (United States)

    1984-08-01

    doppe has been made I* appl the thee ad M etlu ia~ Mdie prblm mowvenng rsu *I wM&#34eqasm then ~ ~ 1 Of cotP MnUMb~ wn Wi pbusha u iutoW i essity ii of...to that iepoee byth vsicle on the soil. in Mhe vnr lfae of Wei soil It wsIspoeiblo to afply the required swot of mnl paseem ian ring shbar testa bemse...idals plasticiLy. Quart. Appl . Nbtb.. Vol.7. No.4. 411-40. IG 4) Hmahiuchi K. ad H. Umn: Elasto-plastic ontitutive laws of arsomlar iterials. Proc. of

  1. Effects of Target Corrosion/Bio Fouling on EMI and Structural Acoustic Signatures in Underwater Environments Acoustic Component Final Report to SERDP MR 2500

    Science.gov (United States)

    2017-06-01

    Contractor with Excet, Inc. Springfield, VA 2215 A. Sarkissian2 2On-Site Contractor with Sotera KeyW Columbia, MD 21046 B.H. Houston and M...34hllm’lr--i1’"\\-,.,,rr-¥.¥’¥fH:tinA--<tt- ctt+t- Ht """""T’’-i ., "C ::, ·~ .)Q...J C • Dislribution authorized to U.S. Government agencies and thelr contractors (check reason below). [ I Fon,ign Govemmenl Information [ I Sollwlnt

  2. Novel Optical Processor for Phased Array Antenna.

    Science.gov (United States)

    1992-10-20

    parallel glass slide into the signal beam optical loop. The parallel glass acts like a variable phase shifter to the signal beam simulating phase drift...A list of possible designs are given as follows , _ _ Velocity fa (100dB/cm) Lumit Wavelength I M2I1 TeO2 Longi 4.2 /m/ns about 3 GHz 1.4 4m 34 Fast...subject to achievable acoustic frequency, the preferred materials are the slow shear wave in TeO2 , the fast shear wave in TeO2 or the shear waves in

  3. Assessment of Maximum Aerobic Capacity and Anaerobic Threshold of Elite Ballet Dancers.

    Science.gov (United States)

    Wyon, Matthew A; Allen, Nick; Cloak, Ross; Beck, Sarah; Davies, Paul; Clarke, Frances

    2016-09-01

    An athlete's cardiorespiratory profile, maximal aerobic capacity, and anaerobic threshold is affected by training regimen and competition demands. The present study aimed to ascertain whether there are company rank differences in maximal aerobic capacity and anaerobic threshold in elite classical ballet dancers. Seventy-four volunteers (M 34, F 40) were recruited from two full-time professional classical ballet companies. All participants completed a continuous incremental treadmill protocol with a 1-km/hr speed increase at the end of each 1-min stage until termination criteria had been achieved (e.g., voluntary cessation, respiratory exchange ratio ballet companies are probably due to the different rehearsal and performance demands.

  4. The Meteorology of Central Africa

    Science.gov (United States)

    1943-11-01

    JxUüha^tiGb-bväSt ’" PiM &#34•" *• i—By taking. law agreosBfe Lhu w:ul- PBJfc3aE5fe„gäfcfia^and fcJa& £ao4 +hn+ *hri rgewfcaga treHr-afcrere i-iin...point to the right and by the constant Ott O-CCoUvit erf; equivalent potential temperature in the lowest layer. « *he low relative humidities

  5. Smart Materials for Army Structures

    Science.gov (United States)

    1992-04-01

    86. . 3 0.0 IMIA8 0 --’O IM S. B .88 . -I 30.0- 10.0 0 .0XI Fig. X4 Electrico o flte i ( eatv 2. andth It 0 / (A) 0-0 [90/0/90] 30.0 u...accomplished computationally, 137 i : D L (B) (C) --- Fig. X~III A demonstration of auto -rotation on a D-shaped blade of a Lancliester propellecr. 138 I...Conductive Polymers and Plastics, Chapman and Hall, 1989. Margolis, J.M., &#34Composites Challenge Metals for Aircraft/ Auto Panel Applications,’ Machine and

  6. Department of the Air Force Justification of Estimates for Fiscal Year 1981, Submitted to Congress January 1980, Aircraft Procurement, Air Force, Missile Procurement, Air Force, Other Procurement, Air Force.

    Science.gov (United States)

    1980-01-01

    10-0 6 EL4 -022 8L LLý 0 0 a 0 CLcc - 3 E CL 0 ;i *1 XO go10 ,* . , -8 rt -C CL.* L. OLc L ’i go 0 0 0 L +1%- 00 00I :) I.- I - - 51 .0 Cc a,,0 c0...of Test Cells M34 and M35. The bldg will be a prefabri- cated steel structure with several work stations. The work stations will have hoist systems...mate/demate the engine to its mount. The work stations will incorporate cell type interfaces for a limited checkout of engine in- strumentation. These

  7. [Connexin gene 26 (GJB2) mutations in patients with hereditary non-syndromic sensorineural loss of hearing in the Republic of Sakha (Yakutia)].

    Science.gov (United States)

    Barashkov, N A; Dzhemileva, L U; Fedorova, S A; Maksimova, N R; Khusnutdinova, E K

    2008-01-01

    The aim of the study was to elucidate the causes of hereditary non-syndromic loss of hearing, a frequent monogene pathology in the Republic of Sakha (Yakutia). A search for mutations in the coding sequence of the connexin 26 gene gap-junction B2 (GJB2) was undertaken in 79 members of 65 unrelated families with the diagnosis of grade III-IV non-syndromic bilateral sensorineural loss of hearing. Five recessive mutations (35delG, V371, 312-326del14, 333-334delAA, R127H) and three polymorphic variants (V271, M34T, E114G) were identified in Yakut patients. Mutations 35delG (41.7%), 312-326dell4 (4.2%), and 333-334delAA (4.2%) were found in Caucasian patients (Russians, Ukrainians, Inguish). Yakuts were carriers of mutations 35delG (2.1%), V371 (2.1%), R127H (1.0%) and sequence variants V271 (6.3%), M34T (1.0%), E114G (1.0%). GJB2 mutations were identified in 50.1% of the Caucasian patients and in 7.2% of the Yakut patients. The low frequency of GJB2 mutations in Yakuts with non-syndromic sensorineural loss of hearing testifies to the presence of mutations of other genes controlling sound perception in this population.

  8. PA-1, a Versatile Anaerobe Obtained in Pure Culture, Catabolizes Benzenoids and Other Compounds in Syntrophy with Hydrogenotrophs, and P-2 plus Wolinella sp. Degrades Benzenoids

    Science.gov (United States)

    Barik, Sudhakar; Brulla, W. J.; Bryant, M. P.

    1985-01-01

    Methanogenic enrichments catabolizing 13 mM phenylacetate or 4 mM phenol were established at 37°C, using a 10% inoculum from a municipal anaerobic digester. By using agar roll tubes of the basal medium plus 0.1% yeast extract-25 mM fumarate, a hydrogenotrophic lawn of Wolinella succinogenes and phenol or phenylacetate, strains P-2 and PA-1, respectively, were isolated in coculture with W. succinogenes. With the lawn deleted, PA-1 was isolated in pure culture. Strain P-2 is apparently a new species of anaerobic, motile, gram-negative, spindle-shaped, small rod that as yet has been grown only in coculture with W. succinogenes. It used phenol, hydrocinnamate, benzoate, and phenylacetate as energy sources. Product recovery by the coculture, per mole of phenol and 4.4 mol of fumarate used, included 2.03, 0.12, 0.08, and 3.23 mol, respectively, of acetate, propionate, butyrate, and succinate. Carbon recovery was 75% and H recovery was 80%, although CO2 and a few other possible products were not determined. That P-2 is an obligate proton-reducing acetogen and possible pathways for its degradation of phenol are discussed. Strain PA-1 is apparently a new species of anaerobic, motile, relatively small, gram-negative rod. It utilized compounds such as phenylacetate, hydrocinnamate, benzoate, phenol, resorcinol, gallate, 4-aminophenol, 2-aminobenzoate, pyruvate, Casamino Acids, and aspartate as energy sources in coculture with W. succinogenes. Per mole of phenylacetate and 1.44 mol of fumarate used, 1.04, 0.53, and 0.78 mol of acetate, propionate, and succinate, respectively, were recovered from the coculture. Only about 50% of the carbon and H were recovered. In coculture with Methanospirillum hungatei, 0.96 mol of acetate and 0.25 mol of methane were recovered per mol of pyruvate used; 0.90 mol of acetate and 0.33 mol of methane, per mol of fumarate used; 0.93 mol of acetate and 0.54 mol of methane, per mol of aspartate used; and 1.71 mol of acetate and 0.57 mol of methane

  9. Nutrição e crescimento do fungo nematófago Arthrobotrys oligospora

    Directory of Open Access Journals (Sweden)

    Eliane R. Cardoso

    2009-12-01

    Full Text Available As condições de crescimento e os requerimentos nutricionais de Arthrobotrys oligospora, um fungo nematófago, foram investigados em meio líquido. O organismo foi incubado em meio sintético, a 30º C e em cultura estacionária. O perfil da curva de crescimento do fungo ajustou-se a uma equação de 3º grau, mesmo após 15 dias de incubação. A temperatura e o pH ótimos para produção de micélio foram observados a 25º C e pH 5,0, respectivamente. Contudo, não foram observadas diferenças significativas entre a produção de biomassa nas temperaturas de 25º C e 30º C ou pH 5.0 e 6.0. Várias fontes de carbono foram utilizadas pelo fungo, porém a maior produção de biomassa foi verificada com maltose e sacarose. Das fontes de nitrogênio testadas, várias proteínas (triptona, extrato de levedura, caseína, peptona e casaminoácidos e fontes inorgânicas (nitrato de sódio e cloreto de amônio estimularam a maior produção de biomassa. Das várias vitaminas experimentadas, o crescimento do fungo aumentou 2,2 vezes com riboflavina e 2,3 vezes com a mistura biotina e tiamina em relação ao controle, sem vitamina. De modo geral, constatou-se, após o período de incubação, que o pH inicial do meio de cultura pode aumentar até 8,4. Estes resultados sugerem que as variáveis estudadas podem ter papel importante no crescimento do organismo no solo.The growth conditions and the nutritional requirements of Arthrobotrys oligospora, a nematophagous fungus, were investigated in liquid culture. The organism was incubated in a synthetic medium, at 30º C in a static culture. Time course of growth was adjusted to a 3º degree equation, even after 15 days of incubation. Optimal temperature and pH for mycelium production were observed at 25º C and pH 5.0, respectively. However, significant differences were not found among the biomass production at temperatures 25º C and 30º C or pH 5.0 and 6.0. Several carbon sources were used by the fungus

  10. Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1β in aerated fed-batch reactor: role of ACA supplementation, strain viability, and maintenance energy

    Directory of Open Access Journals (Sweden)

    Zueco Jesus

    2009-12-01

    Full Text Available Abstract Background Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been used as host for the expression and secretion of human interleukin-1β (IL1β, using the cell wall protein Pir4 as fusion partner. To achieve high cell density and, consequently, high product yield, BY4741 [PIR4-IL1β] was cultured in an aerated fed-batch reactor, using a defined mineral medium supplemented with casamino acids as ACA (auxotrophy-complementing amino acid source. Also the S. cerevisiae mutant BY4741 Δyca1 [PIR4-IL1β], carrying the deletion of the YCA1 gene coding for a caspase-like protein involved in the apoptotic response, was cultured in aerated fed-batch reactor and compared to the parental strain, to test the effect of this mutation on strain robustness. Viability of the producer strains was examined during the runs and a mathematical model, which took into consideration the viable biomass present in the reactor and the glucose consumption for both growth and maintenance, was developed to describe and explain the time-course evolution of the process for both, the BY4741 parental and the BY4741 Δyca1 mutant strain. Results Our results show that the concentrations of ACA in the feeding solution, corresponding to those routinely used in the literature, are limiting for the growth of S. cerevisiae BY4741 [PIR4-IL1β] in fed-batch reactor. Even in the presence of a proper ACA supplementation, S. cerevisiae BY4741 [PIR4-IL1β] did not achieve a high cell density. The Δyca1 deletion did not have a beneficial effect on the overall performance of the strain, but it had a clear effect on its viability, which was not impaired during fed-batch operations, as shown by the kd value (0.0045 h-1, negligible if compared to that of the parental strain (0.028 h-1. However, independently of their robustness, both the parental and the Δyca1 mutant ceased to grow early during fed-batch runs, both strains using most of the

  11. Evaluation of supplementation of sucrose medium on the synthesis of Zymomonas mobilis bio-products = Avaliação da suplementação do meio de sacarose na formação de bio– produtos por Zymomonas mobilis

    Directory of Open Access Journals (Sweden)

    Raquel Renan Jorge Borsari

    2010-07-01

    Full Text Available The effect of the variables pantothenic acid, yeast extract and sodiumchloride, as well as the cell permeabilization technique, were investigated on the formation of levan, ethanol, sorbitol and biomass of Zymomonas mobilis, using a 24-1 fraction factorial design. Cell growth was determined by turbidimetry at 605 nm, relating it to a biomass with a dry weight calibration curve. Reducing sugars were quantified according to Somogyi and Nelson. Total sugars were quantified by the phenol-sulfuric acid method, sorbitol by HPLC and ethanol. The levan produced was precipitated by the addition of absolute ethanol and quantified in fructose units. In levan biosynthesis, the variable that had the largest contribution was cell condition. The results suggested that the factors that most affected biomass and ethanol formation were sodium chloride concentration and cell condition that affected negatively on production. For sorbitol, the variable that had a significant effect was permeabilization, which decreased its synthesis. Studies to amplify the range of established factors would be important.A influência das variáveis: ácido pantotênico, extrato de levedura, cloreto de sódio, e a técnica de permeabilização celular foram investigadas na formação de levana, sorbitol, etanol e biomassa de Zymomonas mobilis utilizando um delineamento estatístico fatorial fracionado 24-1. A biomassa foi determinada por turbidimetria, Os açúcares redutores foram quantificados por Somogy e Nelson, açúcartotal por Fenol Sulfúrico, sorbitol por HPLC e etanol por micro-destilação. A levana produzida foi precipitada com etanol absoluto e determinada como unidade de frutose. Na biossíntese de levana, a variável que mais contribuiu foi a condição celular. Os resultadossugerem que, para a formação da biomassa e etanol, os fatores que mais interferiram foram a concentração de cloreto de sódio e a condição celular que influencia negativamente a produ

  12. Mueller matrix polarimetry for characterizing microstructural variation of nude mouse skin during tissue optical clearing.

    Science.gov (United States)

    Chen, Dongsheng; Zeng, Nan; Xie, Qiaolin; He, Honghui; Tuchin, Valery V; Ma, Hui

    2017-08-01

    We investigate the polarization features corresponding to changes in the microstructure of nude mouse skin during immersion in a glycerol solution. By comparing the Mueller matrix imaging experiments and Monte Carlo simulations, we examine in detail how the Mueller matrix elements vary with the immersion time. The results indicate that the polarization features represented by Mueller matrix elements m22&m33&m44 and the absolute values of m34&m43 are sensitive to the immersion time. To gain a deeper insight on how the microstructures of the skin vary during the tissue optical clearing (TOC), we set up a sphere-cylinder birefringence model (SCBM) of the skin and carry on simulations corresponding to different TOC mechanisms. The good agreement between the experimental and simulated results confirm that Mueller matrix imaging combined with Monte Carlo simulation is potentially a powerful tool for revealing microscopic features of biological tissues.

  13. Design, Synthesis and Antiviral Activity Studies of Schizonepetin Derivatives

    Directory of Open Access Journals (Sweden)

    Anwei Ding

    2013-08-01

    Full Text Available A series of schizonepetin derivatives have been designed and synthesized in order to obtain potent antivirus agents. The antiviral activity against HSV-1 and influenza virus H3N2 as well as the cytotoxicity of these derivatives was evaluated by using cytopathic effect (CPE inhibition assay in vitro. Compounds M2, M4, M5 and M34 showed higher inhibitory activity against HSV-1 virus with the TC50 values being in micromole. Compounds M28, M33, and M35 showed higher inhibitory activity against influenza virus H3N2 with their TC50 values being 96.4, 71.0 and 75.4 μM, respectively. Preliminary biological activity evaluation indicated that the anti-H3N2 and anti-HSV-1 activities improved obviously through the introduction of halogen into the structure of schizonepetin.

  14. Dollar Summary of Prime Contract Awards by State, County, Contractor, and Place. Part 1 (American Samoa - Hawaii), FY1991

    Science.gov (United States)

    1991-01-01

    nA LL IL L 0"<L Ii 6 <Z4 44 44 I. 4.- 4t- W Q 444Ŕ x4 4 4 D- F6 (aIw -- O W J W oj "W M W o W Z ~ J < D_ W: 6-I 0.X 00"w LMoj 00 - 00 0 0 0 000 00...8217) 0 acca 4L In 0,O I (a 1CL I.- 00 I00 400 0 ma0 -A U (04z (no4- O(" 0 44t (<x--w a L a-z I> z---o 000 0)0 N Z O M &#34 " -Jh c F.-< W< 000 0< Iw-Q-< w o

  15. Installation Restoration Program. Phase 2. Confirmation/Quantification. Stage 2. Duluth International Airport, Duluth, Minnesota. Appendix

    Science.gov (United States)

    1988-05-26

    tD o 11 11 11 -l m N1 -0 e a, 1 0...2E z z z z 2 z z z S SO, EB S IED O-. CDI z C z z z CP TD gD 2 i 2Qaniz z z z sa -@ 2N’. Us(~ -- (XJ 3 gN( C’Ea - 2E S00 0 00 00 00 00 00 00 00 0 a 4...dr.f mm, 10 fd, I---_1_~a -- ______________0 5;’~ SOAP- bAL -ft VU.-a& 44L PUMPING LXVEL 4bd .’ lM&#34 -- ) fI ... ______ @-Ph. " o0 3s....4..0 a ir a.m

  16. Review of Cryogenic Mechanical and Thermal Properties of Al-Li Alloys and Alloy 2219

    Science.gov (United States)

    1990-09-01

    I-. 00 CL IVI cc to ,/WeY -S3~o~ 3unow I- I-141 o . S. I : i a z m 2c z3 z 2 z a zz a - aa ao o C b on 0m fm 8f 04 ef Ift 4 4 4 4 0 N 0 0 .4.0m...James, Dennis Rule, and Hassell Ledbetter for providing us with preprints and data. We would also like to thank the many authors who have been so...Space Company, Report No. LAC 07-4774 D, 14 pp. (1989). 10. Welpmann, K., Lee , Y. T., and Peters, M., &#34Low Temperature Deformation Behavior of 8090

  17. K Basin spent fuel sludge treatment alternatives study. Volume 2, Technical options

    International Nuclear Information System (INIS)

    Beary, M.M.; Honekemp, J.R.; Winters, N.

    1995-01-01

    Approximately 2100 metric tons of irradiated N Reactor fuel are stored in the KE and KW Basins at the Hanford Site, Richland, Washington. Corrosion of the fuel has led to the formation of sludges, both within the storage canisters and on the basin floors. Concern about the degraded condition of the fuel and the potential for leakage from the basins in proximity to the Columbia River has resulted in DOE's commitment in the Tri-Party Agreement (TPA) to Milestone M-34-00-T08 to remove the fuel and sludges by a December 2002 target date. To support the planning for this expedited removal action, the implications of sludge management under various scenarios are examined. This report, Volume 2 of two volumes, describes the technical options for managing the sludges, including schedule and cost impacts, and assesses strategies for establishing a preferred path

  18. Establishment and Discontinuance Criteria for Automated Weather Observing Systems (AWOS).

    Science.gov (United States)

    1983-05-01

    supplement the probable cause(s).* Referring back to Figure 20, it is observed that all weat-her cause citations combined from 1975 through 1979 accounted...direction 70 p-rcynt of all arrivals. For the other 30 percent of all arrivals, it i7 r;s-;Lind that the Unicorn is not operating and that no other... vc P. 1W4 ui W Z L C 0e 14 ..t w 0 .- Z) LWWE W>-C" z .. JIL OC I.- -- =Z)- z " -- A tl 0 L- W < uo- z = - e a * w Z0)WI.>Z . - N m =) m &#34 =r P- a3

  19. A Higgs boson candidate decaying into 4 muons in the ATLAS detector

    CERN Multimedia

    ATLAS Collaboration

    2012-01-01

    Event display of a 4mu candidate. EventNumber: 71902630 RunNumber: 204769 m4ell=125.1 GeV. m12=86.3 GeV, m34=31.6 GeV. mu1: pT,eta,phi=36.1 GeV, 1.29, 1.33 . mu2: pT,eta,phi=47.5 GeV, 0.69, -1.65 . mu3: pT,eta,phi=26.4 GeV, 0.47, -2.51 . mu4: pT,eta,phi=71.7 GeV, 1.85, 1.65 . pT4ell= 27.0 GeV. ETmiss=41.8 GeV. (ATLAS-CONF-2012-169)

  20. K Basin spent fuel sludge treatment alternatives study. Volume 2, Technical options

    Energy Technology Data Exchange (ETDEWEB)

    Beary, M.M.; Honekemp, J.R.; Winters, N. [Science Applications International Corp., Richland, WA (United States)

    1995-01-01

    Approximately 2100 metric tons of irradiated N Reactor fuel are stored in the KE and KW Basins at the Hanford Site, Richland, Washington. Corrosion of the fuel has led to the formation of sludges, both within the storage canisters and on the basin floors. Concern about the degraded condition of the fuel and the potential for leakage from the basins in proximity to the Columbia River has resulted in DOE`s commitment in the Tri-Party Agreement (TPA) to Milestone M-34-00-T08 to remove the fuel and sludges by a December 2002 target date. To support the planning for this expedited removal action, the implications of sludge management under various scenarios are examined. This report, Volume 2 of two volumes, describes the technical options for managing the sludges, including schedule and cost impacts, and assesses strategies for establishing a preferred path.

  1. Environmental Impact Statement. Space Nuclear Thermal Propulsion Program. Particle Bed reactor Propulsion Technology Development and Validation

    Science.gov (United States)

    1993-05-01

    EcoripoS 20 Reported byt Neretd St. ThOnsac. CSS No. ISO 25 Ftetd Offiace to Lao Vegas. solvels. hoe. ThO Department of ACCAE PCTMC SAA- S/S-?SASSOCIATED...PropulblonProgram LO =01 a a ulib bso "M &#34Om IS so ffm 0" Idamman wown Swom 0"u "ommi a Say OM "Wame OW Minh itWahm I am a thlvtr- yaw residet ot atd Sawa. I...bW %"-~A, ...Wm pý%JWWd..akopik ~ l~ha.18.711 alae. mn~e OW ~ ffm fM V mW Oft ~ WA&I Alls ..I IMd fW q 1.d 4J 1.20 .dinrapinh- *t.- Php* PW I el.JI*ý

  2. Laser diagnostics of native cervix dabs with human papilloma virus in high carcinogenic risk

    Science.gov (United States)

    Peresunko, O. P.; Karpenko, Ju. G.; Burkovets, D. N.; Ivashko, P. V.; Nikorych, A. V.; Yermolenko, S. B.; Gruia, Ion; Gruia, M. J.

    2015-11-01

    The results of experimental studies of coordinate distributions of Mueller matrix elements of the following types of cervical scraping tissue are presented: rate- low-grade - highly differentiated dysplasia (CIN1-CIN3) - adenocarcinoma of high, medium and low levels of differentiation (G1-G3). The rationale for the choice of statistical points 1-4 orders polarized coherent radiation field, transformed as a result of interaction with the oncologic modified biological layers "epithelium-stroma" as a quantitative criterion of polarimetric optical differentiation state of human biological tissues are shown here. The analysis of the obtained Mueller matrix elements and statistical correlation methods, the systematized by types studied tissues is accomplished. The results of research images of Mueller matrix elements m34 for this type of pathology as low-grade dysplasia (CIN2), the results of its statistical and correlation analysis are presented.

  3. Anomeric 2'-Deoxycytidines and Silver Ions: Hybrid Base Pairs with Greatly Enhanced Stability and Efficient DNA Mismatch Detection with α-dC.

    Science.gov (United States)

    Guo, Xiurong; Seela, Frank

    2017-09-04

    α-d-Nucleosides are rare in nature but can develop fascinating properties when incorporated into DNA. This work reports on the first silver-mediated base pair constructed from two anomeric nucleosides: α-dC and β-dC. The hybrid base pair was integrated into the DNA and DNA/RNA double helix. A 12-mer duplex with α-dC and β-dC pair exhibits a higher thermal stability (T m =43 °C) than that incorporating the β-dC-Ag + -β-dC homo pair (T m =34 °C). Furthermore, α-dC shows excellent mismatch discrimination for DNA single nucleotide polymorphism (SNP). All four SNPs were identified on the basis of large T m value differences measured in the presence of silver ions. High resolution melting was not required. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Evaluation of Used Crankcase Oils Using Computerized Infrared Spectrometry. Appendices.

    Science.gov (United States)

    1984-06-01

    i-yccou" i CL MO iA CM X UOgrg iccr 1 i/l < •4. «J — u- C—« e^csio 1 o> •Cr* lf*M.oP«M.frlO i D «r •tto CP P-Cmif pece m ^ — f»HH M m...34 MO H C • • ^> -HIM^- »—If onmo 1 > 300 1 )300 I • • • I oseo 1 1 • 1 1 •Kim>0 I IS)«« I • • «| -<l r 1 I 1 1 1 1 1 ©ir

  5. Examining the relationship between sex and motivation in triathletes.

    Science.gov (United States)

    López-Fernández, Iván; Merino-Marbán, Rafael; Fernández-Rodríguez, Emilio

    2014-08-01

    The present paper assesses the relationship between sex and motivation in triathletes utilising a multidimensional measurement of motivation in sports. Data were collected from 138 triathlon participants (age range 17-72 years): 95 men (M = 36.7 yr., SD = 10.7) and 43 women (M = 34.6 yr., SD = 9.9). Measures included the Sport Motivation Scale, a multi-dimensional measure to assess different types of athletes' motivations for practising their sport. Results showed a significant sex difference in amotivation, with women displaying lower scores; but amotivation scores were very low. The results were not dependent on competition level or age. Men and women competing at the international level in triathlon have similar motivational profiles.

  6. Plant Equipment Packages: Are they a Credible Deterrent to War

    Science.gov (United States)

    1990-12-01

    PEP# TYPE CONTRACTOR/FACILITY NAME LOCATION 0112 WARHEAD CHAPARRAL M250 0112 WARHEAD HELFIRE MISSILE SYSTEM 0112 WARHEAD HE M225 LAP (DRAGON) 0112...0149 GRENADE SMK SCRN RP UKL8A3 F/ M250 LAUNCHER 0149 ROCKET 66MM INCEND TPA 4RD CLIP M74 0149 WARHEAD RKT 2.75IN SMK WP M156 W/FZ PD M427 0149 ROCKET...M25A2 (COMPLETE) 0149 GRENADE HAND/RIFLE SMK WP M34 W/F M206A2. 0149 GRENADE HAND RIOT CS ABC-M7A3 W/FZ M201AI 0149 GRENADE SMK SCRN RP UKL8A3 F/ M250

  7. An Air Force History of Space Activities, 1945-1959

    Science.gov (United States)

    1964-08-01

    110 .uch Ol’JI081 t1oa tbat theT woul.cl 1"’M1.l¥ 10M.&#34 vu.oa buecl ·111. optD\\ia oa tb8 Air Porn , wbich b8 4Mcnbe4 u "tile but 1D the world." ·It... Arab Bap.lbl.ic1 Ntuaecl. to. par- . . t1c1pate becaWie tbe ••·ŖMI 1Nt1𔄂 ezcluU41 8114 tbe 18 •Iaber co.ittee * . tlua --. a ].3-.-ber cc.itt.ee 1D...Mexico, PolaDd, RumaD1a, Swedea, the Soviet UDioa, tbe UD1te4 Arab Be- pub].j,c_, the United JC1as4aa, aDd tbe United State1. ’ f • 1 • • I 1 I

  8. Personal self-concept and satisfaction with life in adolescence, youth and adulthood.

    Science.gov (United States)

    Goñi Palacios, Eider; Esnaola Echaniz, Igor; Rodríguez Fernández, Arantzazu; Camino Ortiz de Barrón, Igor

    2015-01-01

    The main aim of this study was to explore the relationships between personal self-concept and satisfaction with life, with the latter as the key indicator for personal adjustment. The study tests a structural model which encompasses four dimensions of self-concept: self-fulfillment, autonomy, honesty and emotions. The 801 participants in the study, all of whom were aged between 15 and 65 (M = 34.03, SD = 17.29), completed the Satisfaction with Life Scale (SWLS) and the Personal Self-Concept (APE) Questionnaire. Although the four dimensions of personal self-concept differ in their weight, the results show that, taken together, they explain 46% of the differences observed in satisfaction with life. This implies a weight that is as significant as that observed for general self-esteem in previous research studies. This issue should be dealt with early on, during secondary education, in order to help prevent psychological distress or maladjustment.

  9. Spent fuel consolidation in the 105KW Building fuel storage basin

    International Nuclear Information System (INIS)

    Johnson, B.H.

    1994-01-01

    This study is one element of a larger engineering study effort by WHC to examine the feasibility of irradiated fuel and sludge consolidation in the KW Basin in response to TPA Milestone (target date) M-34-00-T03. The study concludes that up to 11,500 fuel storage canisters could be accommodated in the KW Basin with modifications. These modifications would include provisions for multi-tiered canister storage involving the fabrication and installation of new storage racks and installation of additional decay heat removal systems for control of basin water temperature. The ability of existing systems to control radionuclide concentrations in the basin water is examined. The study discusses requirements for spent nuclear fuel inventory given the proposed multi-tiered storage arrangement, the impact of the consolidated mass on the KW Basin structure, and criticality issues associated with multi-tiered storage

  10. Base Structure Annex to Manpower Requirements Report for FY 1982.

    Science.gov (United States)

    1981-01-01

    1 0 x71 4 (1) w: 1- 1 0L)0 L) D -i w < - I-1 341 41) > 1- W W .1 a: LU -14 4 cW - ) < ( (a: UW -3: LLL 0 0 > 403 01- m 1101 - Z - WLUILL a: zaW LU D...8217h ir It’" t-di’p r ~ V I y> m &#34’ Ř)t’(’.’ll /Mn t t Air . 1r mud to adi ’, -oIe (I in t m ir- tn-o ’ i~~ ~ -P~h " I’ ’ii ,I i’ , in ne) !, tun ~Ot

  11. Arctic Ice Dynamics Joint Experiment 1975-1976. Physical Oceanography Data Report, Salinity, Temperature and Depth Data, Camp Caribou. Volume I.

    Science.gov (United States)

    1980-02-01

    0 wr NM 1 NNNN .VIDT00m 00~ V"!8% 00 WVm Mmp 08NN M8MM f"i M0 Ott " -n-nn ON & MM 84. 0’IM-0 Ic 5-0 0 . .8. . ....... .. o noemn l I.50n MM MM.f m.MN...0 .4-O"s- W oo P Sn .0o %.in-0 06"l 00l-o 0--P- s-fp.P ?o’ 0-0~- .. Me5U MM5i MW We M Pa- 0* PIM - .4 ൈM&#34 IMts...8217 It T- Ott , Tq 5 :Tn.a- 0 a11-0 T .:C UW-0- C t LJ34 0 -M N MM ~ ~ lM 44 Irvf: 555. 55?? o0 02 :t!:: 4 ljWOWU"M, n HO I 1010. . X 000..4~Iin00.0. 50

  12. K Basin spent fuel sludge treatment alternatives study. Volume 1, Regulatory options

    International Nuclear Information System (INIS)

    Beary, M.M.; Honekemp, J.R.; Winters, N.

    1995-01-01

    Approximately 2100 metric tons of irradiated N Reactor fuel are stored in the KE and KW Basins at the Hanford Site, Richland, Washington. Corrosion of the fuel has led to the formation of sludges, both within the storage canisters and on the basin floors. Concern about the degraded condition of the fuel and the potential for leakage from the basins in proximity to the Columbia River has resulted in DOE's commitment in the Tri-Party Agreement (TPA) to Milestone M-34-00-T08 to remove the fuel and sludges by a December 2002 target date. To support the planning for this expedited removal action, the implications of sludge management under various scenarios are examined. Volume 1 of this two-volume report describes the regulatory options for managing the sludges, including schedule and cost impacts, and assesses strategies for establishing a preferred path

  13. Compilation of 1986 Annual Reports of the Navy ELF (Extremely Low Frequency) Communications System Ecological Monitoring Program. Volume 3. TABS H-J.

    Science.gov (United States)

    1987-07-01

    SLta Lii &4"Kg (?1I.WS tIW 蚌Z~ ~ ~ .. A~j’ 0Ś. Thm PruL 1*L*uY da U4- UW arm W"A * k~ ft.t . a*Ik L A Vm &’ we_ em_ do .f OWx*4 wk VIVW. *am...treatment transects. Among sFx-eis that were tested in .- "-- .a.’Sr WE nu, xANMw M&#34It6T U UPWTW rYW F-,T1., Mr~ T n- WVIn UVA V1w ?%n K’ -xr F 1flWW WVWWY...Fruit 15 Foliage insects and seeds 16 Bark insects 17 Nectar and sap 18 Aquatic vegetation 19 Aquatic invertebrates C. Habitat 1 Deciduous forest 2

  14. The Compleat TRAIDsman.

    Science.gov (United States)

    1969-09-01

    uses a COMMON block of 663 cells . PLOTT works from a table of data, and assigns to each trace (up to 6 traces) a preset character. Once yoor data is...ALFA 000777 1 ~L P M A LP A U VU I 00) 󈧑 EL4 ’ I’LAC 4-43 FLIER slU.-r t;IINk fLIE" (5,LP.KONUA IlEI 335 50C k k LJ~C I~ N’.12 5 ExTEN, REVISION of...FLAG, PLACLD IN CumM&#34O. 753 C N3.0,0 AS RUN OAIE (IF BLANK* TAKEN FROM SYSTEM C DATE CELL ) ?SS C e1-63 13 EXECUTION TIME LIMIT IN MINUTES (IF ?b6 C

  15. The Mars Science Laboratory Curiosity rover Mastcam instruments: Preflight and in-flight calibration, validation, and data archiving

    Science.gov (United States)

    Bell, James F.; Godber, A.; McNair, S.; Caplinger, M.A.; Maki, J.N.; Lemmon, M.T.; Van Beek, J.; Malin, M.C.; Wellington, D.; Kinch, K.M.; Madsen, M.B.; Hardgrove, C.; Ravine, M.A.; Jensen, E.; Harker, D.; Anderson, Ryan; Herkenhoff, Kenneth E.; Morris, R.V.; Cisneros, E.; Deen, R.G.

    2017-01-01

    The NASA Curiosity rover Mast Camera (Mastcam) system is a pair of fixed-focal length, multispectral, color CCD imagers mounted ~2 m above the surface on the rover's remote sensing mast, along with associated electronics and an onboard calibration target. The left Mastcam (M-34) has a 34 mm focal length, an instantaneous field of view (IFOV) of 0.22 mrad, and a FOV of 20° × 15° over the full 1648 × 1200 pixel span of its Kodak KAI-2020 CCD. The right Mastcam (M-100) has a 100 mm focal length, an IFOV of 0.074 mrad, and a FOV of 6.8° × 5.1° using the same detector. The cameras are separated by 24.2 cm on the mast, allowing stereo images to be obtained at the resolution of the M-34 camera. Each camera has an eight-position filter wheel, enabling it to take Bayer pattern red, green, and blue (RGB) “true color” images, multispectral images in nine additional bands spanning ~400–1100 nm, and images of the Sun in two colors through neutral density-coated filters. An associated Digital Electronics Assembly provides command and data interfaces to the rover, 8 Gb of image storage per camera, 11 bit to 8 bit companding, JPEG compression, and acquisition of high-definition video. Here we describe the preflight and in-flight calibration of Mastcam images, the ways that they are being archived in the NASA Planetary Data System, and the ways that calibration refinements are being developed as the investigation progresses on Mars. We also provide some examples of data sets and analyses that help to validate the accuracy and precision of the calibration

  16. The Mars Science Laboratory Curiosity rover Mastcam instruments: Preflight and in-flight calibration, validation, and data archiving

    Science.gov (United States)

    Bell, J. F.; Godber, A.; McNair, S.; Caplinger, M. A.; Maki, J. N.; Lemmon, M. T.; Van Beek, J.; Malin, M. C.; Wellington, D.; Kinch, K. M.; Madsen, M. B.; Hardgrove, C.; Ravine, M. A.; Jensen, E.; Harker, D.; Anderson, R. B.; Herkenhoff, K. E.; Morris, R. V.; Cisneros, E.; Deen, R. G.

    2017-07-01

    The NASA Curiosity rover Mast Camera (Mastcam) system is a pair of fixed-focal length, multispectral, color CCD imagers mounted 2 m above the surface on the rover's remote sensing mast, along with associated electronics and an onboard calibration target. The left Mastcam (M-34) has a 34 mm focal length, an instantaneous field of view (IFOV) of 0.22 mrad, and a FOV of 20° × 15° over the full 1648 × 1200 pixel span of its Kodak KAI-2020 CCD. The right Mastcam (M-100) has a 100 mm focal length, an IFOV of 0.074 mrad, and a FOV of 6.8° × 5.1° using the same detector. The cameras are separated by 24.2 cm on the mast, allowing stereo images to be obtained at the resolution of the M-34 camera. Each camera has an eight-position filter wheel, enabling it to take Bayer pattern red, green, and blue (RGB) "true color" images, multispectral images in nine additional bands spanning 400-1100 nm, and images of the Sun in two colors through neutral density-coated filters. An associated Digital Electronics Assembly provides command and data interfaces to the rover, 8 Gb of image storage per camera, 11 bit to 8 bit companding, JPEG compression, and acquisition of high-definition video. Here we describe the preflight and in-flight calibration of Mastcam images, the ways that they are being archived in the NASA Planetary Data System, and the ways that calibration refinements are being developed as the investigation progresses on Mars. We also provide some examples of data sets and analyses that help to validate the accuracy and precision of the calibration.

  17. The Mars Science Laboratory (MSL) Mast cameras and Descent imager: Investigation and instrument descriptions

    Science.gov (United States)

    Malin, Michal C.; Ravine, Michael A.; Caplinger, Michael A.; Tony Ghaemi, F.; Schaffner, Jacob A.; Maki, Justin N.; Bell, James F.; Cameron, James F.; Dietrich, William E.; Edgett, Kenneth S.; Edwards, Laurence J.; Garvin, James B.; Hallet, Bernard; Herkenhoff, Kenneth E.; Heydari, Ezat; Kah, Linda C.; Lemmon, Mark T.; Minitti, Michelle E.; Olson, Timothy S.; Parker, Timothy J.; Rowland, Scott K.; Schieber, Juergen; Sletten, Ron; Sullivan, Robert J.; Sumner, Dawn Y.; Aileen Yingst, R.; Duston, Brian M.; McNair, Sean; Jensen, Elsa H.

    2017-08-01

    The Mars Science Laboratory Mast camera and Descent Imager investigations were designed, built, and operated by Malin Space Science Systems of San Diego, CA. They share common electronics and focal plane designs but have different optics. There are two Mastcams of dissimilar focal length. The Mastcam-34 has an f/8, 34 mm focal length lens, and the M-100 an f/10, 100 mm focal length lens. The M-34 field of view is about 20° × 15° with an instantaneous field of view (IFOV) of 218 μrad; the M-100 field of view (FOV) is 6.8° × 5.1° with an IFOV of 74 μrad. The M-34 can focus from 0.5 m to infinity, and the M-100 from 1.6 m to infinity. All three cameras can acquire color images through a Bayer color filter array, and the Mastcams can also acquire images through seven science filters. Images are ≤1600 pixels wide by 1200 pixels tall. The Mastcams, mounted on the 2 m tall Remote Sensing Mast, have a 360° azimuth and 180° elevation field of regard. Mars Descent Imager is fixed-mounted to the bottom left front side of the rover at 66 cm above the surface. Its fixed focus lens is in focus from 2 m to infinity, but out of focus at 66 cm. The f/3 lens has a FOV of 70° by 52° across and along the direction of motion, with an IFOV of 0.76 mrad. All cameras can acquire video at 4 frames/second for full frames or 720p HD at 6 fps. Images can be processed using lossy Joint Photographic Experts Group and predictive lossless compression.

  18. Functional cardiovascular action of L-cysteine microinjected into pressor sites of the rostral ventrolateral medulla of the rat.

    Science.gov (United States)

    Takemoto, Yumi

    2014-04-01

    The endogenous sulfur-containing amino acid L-cysteine injected into the cerebrospinal fluid space of the cisterna magna increases arterial blood pressure (ABP) and heart rate (HR) in the freely moving rat. The present study examined (1) cardiovascular responses to L-cysteine microinjected into the rostral ventrolateral medulla (RVLM), where a group of neurons regulate activities of cardiovascular sympathetic neurons and (2) involvement of ionotropic excitatory amino acid (iEAA) receptors in response. In the RVLM of urethane-anesthetized rats accessed ventrally and identified with pressor responses to L-glutamate (10 mM, 34 nl), microinjections of L-cysteine increased ABP and HR dose dependently (3-100 mM, 34 nl). The cardiovascular responses to L-cysteine (30 mM) were not attenuated by a prior injection of either antagonist alone, MK801 (20 mM, 68 nl) for the NMDA type of iEAA receptors, or CNQX (2 mM) for the non-NMDA type. However, inhibition of both NMDA and non-NMDA receptors with additional prior injection of either antagonist completely blocked those responses to L-cysteine. The results indicate that L-cysteine has functional cardiovascular action in the RVLM of the anesthetized rat, and the responses to L-cysteine involve both NMDA and non-NMDA receptors albeit in a mutually exclusive parallel fashion. The findings may suggest endogenous roles of L-cysteine indirectly via iEAA receptors in the neuronal network of the RVLM for cardiovascular regulation in physiological and pathological situations.

  19. Measuring premorbid IQ in traumatic brain injury: an examination of the validity of the Wechsler Test of Adult Reading (WTAR).

    Science.gov (United States)

    Green, Robin E A; Melo, Brenda; Christensen, Bruce; Ngo, Le-Anh; Monette, Georges; Bradbury, Cheryl

    2008-02-01

    Estimation of premorbid IQ in traumatic brain injury (TBI) is clinically and scientifically valuable because it permits the quantification of the cognitive impact of injury. This is achieved by comparing performances on tests of current ability to estimates of premorbid IQ, thereby enabling current capacity to be interpreted in light of preinjury ability. However, the validity of premorbid IQ tests that are commonly used for TBI has been questioned. In the present study, we examined the psychometric properties of a recently developed test, the Wechsler Test of Adult Reading (WTAR), which has yet to be examined for TBI. The cognitive performance of a group of 24 patients recovering from TBI (with a mean Glasgow Coma Scale score in the severely impaired range) was measured at 2 and 5 months postinjury. On both occasions, patients were administered three tests that have been used to measure premorbid IQ (the WTAR and the Vocabulary and Matrix Reasoning subtests of the Wechsler Adult Intelligence Scale 3rd Edition, WAIS-III) and three tests of current ability (Symbol Digit Modalities Test-Oral and Similarities and Block Design subtests of the WAIS-III). We found that performance significantly improved on tests of current cognitive ability, confirming recovery. In contrast, stable performance was observed on the WTAR from Assessment 1 (M = 34.25/50) to Assessment 2 (M = 34.21/50; r = .970, p tests are indicated (i.e., in patients for whom English is spoken and read fluently), these results endorse the use of the WTAR for patients with TBI.

  20. Open radical cystectomy in England: the current standard of care - an analysis of the British Association of Urological Surgeons (BAUS) cystectomy audit and Hospital Episodes Statistics (HES) data.

    Science.gov (United States)

    Jefferies, Edward R; Cresswell, Joanne; McGrath, John S; Miller, Catherine; Hounsome, Luke; Fowler, Sarah; Rowe, Edward W

    2018-01-23

    To establish the current standard for open radical cystectomy (ORC) in England, as data entry by surgeons performing RC to the British Association of Urological Surgeons (BAUS) database was mandated in 2013 and combining this with Hospital Episodes Statistics (HES) data has allowed comprehensive outcome analysis for the first time. All patients were included in this analysis if they were uploaded to the BAUS data registry and reported to have been performed in the 2 years between 1 January 2014 and 31 December 2015 in England (from mandate onwards) and had been documented as being performed in an open fashion (not laparoscopic, robot assisted or the technique field left blank). The HES data were accessed via the HES website. Office of Population Censuses and Surveys Classification of Surgical Operations and Procedures version 4 (OPCS-4) Code M34 was searched during the same 2-year time frame (not including M34.4 for simple cystectomy or with additional minimal access codes Y75.1-9 documenting a laparoscopic or robotic approach was used) to assess data capture. A total of 2 537 ORCs were recorded in the BAUS registry and 3 043 in the HES data. This indicates a capture rate of 83.4% of all cases. The median operative time was 5 h, harvesting a median of 11-20 lymph nodes, with a median blood loss of 500-1 000 mL, and a transfusion rate of 21.8%. The median length of stay was 11 days, with a 30-day mortality rate of 1.58%. This is the largest, contemporary cohort of ORCs in England, encompassing >80% of all performed operations. We now know the current standard for ORC in England. This provides the basis for individual surgeons and units to compare their outcomes and a standard with which future techniques and modifications can be compared. © 2018 The Authors BJU International © 2018 BJU International Published by John Wiley & Sons Ltd.

  1. Spectrophotometric Modeling of MAHLI Goniometer Observations

    Science.gov (United States)

    Liang, W.; Johnson, J. R.; Hayes, A.; Lemmon, M. T.; Bell, J. F., III; Grundy, W. M.; Deen, R. G.

    2017-12-01

    The Mars Hand Lends Imager (MAHLI) on the Curiosity rover's robotic arm was used as a goniometer to acquire a multiple-viewpoint data set on sol 544 [1]. Images were acquired at 20 arm positions, all centered at the same location and from a near-constant distance of 1.0 m from the surface. Although this sequence was acquired at only one time of day ( 13:30 LTST), it provided phase angle coverage from 0-110°. Images were converted to radiance from calibrated PDS files (DRXX) using radiance scaling factors and MAHLI focus position counts in an algorithm that rescaled the data to match the Mastcam M-34 calibration via comparison of sky images acquired during the mission. Converted MAHLI radiance values from an image of the Mastcam calibration target compared favorably in the red, green, and blue Bayer filters to M-34 radiance values from an image of the same target taken minutes afterwards. The 20 MAHLI images allowed construction of a digital terrain model (DTM), although images with shadows cast by the rover arm were more challenging to include. Their current absence restricts the lowest phase angles available to about 17°. The DTM enables calculation of surface normals that can be used with sky models to correct for diffuse reflectance on surface facets prior to Hapke modeling [cf. 2-6]. Regions of interest (ROIs) were extracted using one of the low emission-angle images as a template. ROI unit types included soils, light-toned surfaces (5 cm felsic rock "Nita"), dark-toned rocks with variable textures and dust cover, and larger areas representative of the average surface (see attached figure). These ROIs were translated from the template image to the other images through a matching of DTM three-dimensional coordinates. Preliminary phase curves (prior to atmospheric correction) show that soil-dominated surfaces are most backscattering, whereas rocks are least backscattering, and light-toned surfaces exhibit wavelength-dependent scattering. Future work will

  2. Helicopter Flying Qualities Characteristics-CH-46E. Volume 4.

    Science.gov (United States)

    1983-10-03

    In UC in., WP.. o"N 05% w 0 ILw60V U.UNt’- =0 GAO 4 NCw-I W%?qWee -I.al OMMM aZ1. Z1 I- -- 4 >m Enl MwCe 0 0 40-1 m60’ Now"’%6 MMMM 60 41 .0 T in g n...1- %-r U. LL 4N WWN= ILL w e40 U.ww =I imn kn = %. E ’a 4C4 M N CD 0%r %r NN N % C C 0..6. * W x Xi W N X.&ON a0.. JinI ON ..1.-AV".-4 ww0-. M’ W...I="Iv K~~ I I1II . 4 ..a.a~a gao ma Qi co 0MMO =4Mp~~ &Ww Wa. 0P ~w ":m II mme Od a - amaac c44 c44 0 1% CY an-$ U.s .. r (M &#34 ty U**@@**~ ~ ~ a

  3. Multiple measures of physical activity, dietary habits and weight status in African American and Hispanic or Latina women.

    Science.gov (United States)

    Lee, Rebecca E; Mama, Scherezade K; Medina, Ashley V; Reese-Smith, Jacqueline Y; Banda, Jorge A; Layne, Charles S; Baxter, Meggin; O'Connor, Daniel P; McNeill, Lorna; Estabrooks, Paul A

    2011-12-01

    Compared measures of physical activity and dietary habits used in the Health Is Power (HIP) study, and described the associations of physical activity and dietary habits among African American and Hispanic or Latino women, adjusted for weight status. Cross-sectional baseline data were compared for community dwelling, healthy African American (N = 262) and Hispanic or Latina women (N = 148) who participated in HIP. Physical activity was measured using the International Physical Activity Questionnaire (IPAQ) long form, the Check And Line Questionnaire (CALQ) log and accelerometry. Dietary habits were measured using NCI 24-h recall screeners, vegetable and fruit (VF) logs and the NCI Diet History Questionnaire (DHQ). Differences in physical activity and dietary habits were assessed using simultaneous 2 (ethnicity) × 3 (weight status) ANCOVAs adjusted for age and socioeconomic status. Women (M age = 44.4 ± 10.9 years) were obese (M = 34.0 ± 9.7 kg/m(2)), did not meet physical activity guidelines as measured by accelerometry (M = 19.4 ± 19.1 min MVPA/day) and ate few VF (M = 2.8 ± 2.7 servings/day). DHQ variables differed by weight status. IPAQ was associated with CALQ, and CALQ with accelerometry (P habits depending on measure used.

  4. Fracture toughness behavior of irradiated stainless steel in PWR systems

    Energy Technology Data Exchange (ETDEWEB)

    Xu, H.; Fyfitch, S. [AREVA NP Inc., Lynchburg, Pennsylvania (United States); Tang, H.T. [Electric Power Research Inst., Palo Alto, California (United States)

    2007-07-01

    Data from available research programs were collected and evaluated by the Electric Power Research Institute (EPRI) Materials Reliability Program (MRP) to determine the relationship between fracture toughness and neutron fluence for conditions representative of pressurized water reactor (PWR) conditions. It is shown that the reduction of fracture toughness with increasing neutron dose in both boiling water reactors (BWRs) and PWRs is consistent with that observed in fast reactors. The lower bound fracture toughness observed for irradiated stainless steels in PWRs is 38 MPa{radical}m (34.6 ksi{radical}in) at neutron exposures greater than 6.7 X 10{sup 21} n/cm{sup 2} (E > 1.0 MeV) or approximately 10 dpa. For such levels of fracture toughness, it is recommended that linear-elastic fracture mechanics (LEFM) analyses be considered for design and operational analyses. The results from this study can be used by the nuclear industry to assess the effects of irradiation on stainless steels in PWR systems. (author)

  5. Amplitude and rise time compensated timing optimized for large semiconductor detectors

    International Nuclear Information System (INIS)

    Kozyczkowski, J.J.; Bialkowski, J.

    1976-01-01

    The ARC timing described has excellent timing properties even when using a wide range e.g. from 10 keV to over 1 MeV. The detector signal from a preamplifier is accepted directly by the unit as a timing filter amplifier with a sensitivity of 1 mV is incorporated. The adjustable rise time rejection feature makes it possible to achieve a good prompt time spectrum with symmetrical exponential shape down to less than 1/100 of the peak value. A complete block diagram of the unit is given together with results of extensive tests of its performance. For example the time spectrum for (1330+-20) keV of 60 Co taken with a 43 cm 3 Ge(Li) detector has the following parameters: fwhm = 2.2ns, fwtm = 4.4 ns and fw (0.01) m = 7.6 ns and for (50 +- 10) keV of 22 Na the following was obtained: fwhm = 10.8 ns, fwtm = 21.6 ns and fw (0.01) m = 34.6 ns. In another experiment with two fast plastic scintillations (NE 102A) and using a 20% dynamic energy range the following was measured: fwhm = 280 ps, fwtm = 470 ps and fw (0.01) m = 70ps. (Auth.)

  6. The Relationship between the Physical Activity Environment, Nature Relatedness, Anxiety, and the Psychological Well-being Benefits of Regular Exercisers

    Directory of Open Access Journals (Sweden)

    Emma Lawton

    2017-06-01

    Full Text Available Research from a variety of scientific fields suggests that physical activity in nature and feelings of connection to nature enhance psychological health and well-being. This study investigated the psychological health and well-being impact of the physical activity environment for those already undertaking the recommended weekly amount of physical activity. This topic is important for the design of health and well-being environments and interventions involving physical activity. Participants (N = 262 aged 18–71 years (M = 34.5, SD = 13.1 who met the UK physical activity guidelines completed the Nature Relatedness Scale, the trait section of the State Trait Inventory for Cognitive and Somatic Anxiety and the Psychological Well-Being Scale. Analysis via Multivariate ANOVA indicated that participants who engaged in outdoor physical activity reported significantly lower somatic anxiety levels and higher Nature Relatedness experience (NRexp. Significant results were not evident for wellbeing. Hierarchical regressions revealed that the psychological well-being facet of autonomy, NRexp, and outdoor physical activity predicted lower somatic anxiety, whereas indoor physical activity predicted higher somatic anxiety. Results indicate that somatic anxiety is lower for outdoor physical activity participation, and that outdoor activity, in conjunction with autonomy and NRexp, predicts lower anxiety levels. The findings extend previous work by demonstrating the impact of the physical activity environment on anxiety levels, as well as the contribution of outdoor physical activity and well-being facets to the previously established Nature Relatedness-anxiety relationship.

  7. ESTIMATING MORAL DEVELOPMENT IN A SAMPLE OF ALTERNATIVE FORMULAS OF PRISON SENTENCED BENEFICIARIES FROM WEST VENEZUELA

    Directory of Open Access Journals (Sweden)

    CARLOS ENRIQUE ZERPA

    2006-05-01

    Full Text Available The level of moral development in a sampled of convicts from West Venezuela is presented. The studywas carried out by means of Defining Issues Test (DIT evaluation. The sample includes 50 persons between19 and 55 years old (M = 34,33 y DT = 8,76, out of them 39 were males (average age 33,74,DT=8,24 an 11 were females (average age 35,91, DT=10,67. All of them were beneficiaries of alternativesformulas of their respective sentences such as social work, parole or conditional suspension ofsentences and following the corresponding process or rehabilitation out of prisons. This sample registereda moral principles index of 19,67 and a non-relevant answers index of 12,07 as well as fourthmoral developmental stage of 41,3 which are unusually high. The results suggest that there is not astatistically significant difference in the pattern of moral development depending on sex, age, or institutionfor sentenced fulfillment. However, the unusual high pattern of answers and the inverse correspondence between the duration of sentences and the resulting level of moral principles suggest that thesample behaves in a way, which is different from what is expected in no-out-law samples. Findings andimplications are discused.

  8. Beliefs and attitudes towards mental illness: an examination of the sex differences in mental health literacy in a community sample

    Directory of Open Access Journals (Sweden)

    Raymond J. Gibbons

    2015-06-01

    Full Text Available Objectives. The current study investigated mental health literacy in an Australian sample to examine sex differences in the identification of and attitudes towards various aspects of mental illness.Method. An online questionnaire was completed by 373 participants (M = 34.87 years. Participants were randomly assigned either a male or female version of a vignette depicting an individual exhibiting the symptoms of one of three types of mental illness (depression, anxiety, or psychosis and asked to answer questions relating to aspects of mental health literacy.Results. Males exhibited poorer mental health literacy skills compared to females. Males were less likely to correctly identify the type of mental illness, more likely to rate symptoms as less serious, to perceive the individual as having greater personal control over such symptoms, and less likely to endorse the need for treatment for anxiety or psychosis.Conclusion. Generally, the sample was relatively proficient at correctly identifying mental illness but overall males displayed poorer mental health literacy skills than females.

  9. Characteristics of internet use as a means of perceived stress reduction in health care students

    Directory of Open Access Journals (Sweden)

    Hinić Darko

    2016-01-01

    Full Text Available Previous studies suggest that people use media for instrumental needs, entertainment/leisure or for social gratification. The aim of this study is to identify specific characteristics of Internet use in health care students who use the Internet as a means of perceived stress reduction. The sample included 231 students (m=34%; f=66%, aged (M=22.44; SD=2.09, who were subsequently categorised into two groups according to whether they consciously use the Internet to alleviate the consequences of stressful experiences. The individuals reporting the Internet as a means of perceived stress reduction spend considerably more time online than other Internet users. They also use contents related to entertainment, culture and sex more frequently, as well as social applications and networks. In comparison to others, these participants meet new people and potential partners more often via the Internet, spend more time on their friends' profiles, while using different entertainment applications, most commonly games. The Internet may play a significant role in reducing negative reactions to stressful events; therefore, we may regard cyberspace as a fertile ground for educational, preventive and counselling services. The main limitation to the current study is the sample (only health care students. Future studies should include assessment of perceived stress levels and their relation to study variables.

  10. Usefulness of MLPA in the detection of SHOX deletions.

    Science.gov (United States)

    Funari, Mariana F A; Jorge, Alexander A L; Souza, Silvia C A L; Billerbeck, Ana E C; Arnhold, Ivo J P; Mendonca, Berenice B; Nishi, Mirian Y

    2010-01-01

    SHOX haploinsufficiency causes a wide spectrum of short stature phenotypes, such as Leri-Weill dyschondrosteosis (LWD) and disproportionate short stature (DSS). SHOX deletions are responsible for approximately two thirds of isolated haploinsufficiency; therefore, it is important to determine the most appropriate methodology for detection of gene deletion. In this study, three methodologies for the detection of SHOX deletions were compared: the fluorescence in situ hybridization (FISH), microsatellite analysis and multiplex ligation-dependent probe amplification (MLPA). Forty-four patients (8 LWD and 36 DSS) were analyzed. The cosmid LLNOYCO3'M'34F5 was used as a probe for the FISH analysis and microsatellite analysis were performed using three intragenic microsatellite markers. MLPA was performed using commercial kits. Twelve patients (8 LWD and 4 DSS) had deletions in SHOX area detected by MLPA and 2 patients generated discordant results with the other methodologies. In the first case, the deletion was not detected by FISH. In the second case, both FISH and microsatellite analyses were unable to identify the intragenic deletion. In conclusion, MLPA was more sensitive, less expensive and less laborious; therefore, it should be used as the initial molecular method for the detection of SHOX gene deletion. Copyright © 2010 Elsevier Masson SAS. All rights reserved.

  11. Noncollinear antiferromagnetic Mn3Sn films

    Science.gov (United States)

    Markou, A.; Taylor, J. M.; Kalache, A.; Werner, P.; Parkin, S. S. P.; Felser, C.

    2018-05-01

    Noncollinear hexagonal antiferromagnets with almost zero net magnetization were recently shown to demonstrate giant anomalous Hall effect. Here, we present the structural and magnetic properties of noncollinear antiferromagnetic Mn3Sn thin films heteroepitaxially grown on Y:ZrO2 (111) substrates with a Ru underlayer. The Mn3Sn films were crystallized in the hexagonal D 019 structure with c -axis preferred (0001) crystal orientation. The Mn3Sn films are discontinuous, forming large islands of approximately 400 nm in width, but are chemical homogeneous and characterized by near perfect heteroepitaxy. Furthermore, the thin films show weak ferromagnetism with an in-plane uncompensated magnetization of M =34 kA/m and coercivity of μ0Hc=4.0 mT at room temperature. Additionally, the exchange bias effect was studied in Mn3Sn /Py bilayers. Exchange bias fields up to μ0HEB=12.6 mT can be achieved at 5 K. These results show Mn3Sn films to be an attractive material for applications in antiferromagnetic spintronics.

  12. Tin oxide quantum dots embedded iron oxide composite as efficient lead sensor

    Science.gov (United States)

    Dutta, Dipa; Bahadur, Dhirendra

    2018-04-01

    SnO2 quantum dots (QDs) embedded iron oxide (IO) nanocomposite is fabricated and explored as a capable sensor for lead detection. Square wave anodic stripping voltammetry (SWASV) and amperometry have been used to explore the proposed sensor's response towards lead detection. The modified electrode shows linear current response for concentration of lead ranging from 99 nM to 6.6 µM with limit of detection 0.42 µM (34 ppb). Amperometry shows a detection limit as low as 0.18 nM (0.015 ppb); which is far below the permissible limit of lead in drinking water by World Health Organization. This proposed sensor shows linear current response (R2 = 0.98) for the lead concentration ranging from 133 × 10-9 to 4.4 × 10-6M. It also exhibits rapid response time of 12 sec with an ultra high sensitivity of 5.5 µA/nM. These detection properties promise the use of SnO2 QDs -IO composite for detection of lead in environmental sample with great ease.

  13. Prime Contract Awards Alphabetically by Contractor, State or Country, and Place. Part 12 (McFadden Jimmy, Portugal - NCR Japan LTD, Japan), FY1991

    Science.gov (United States)

    1991-01-01

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  14. Prime Contract Awards Alphabetically by Contractor, State or Country, and Place. Part 16 (South Dakota [State of], Brookings, South Dakota - Tekna, Mountain View, California), FY1991

    Science.gov (United States)

    1991-01-01

    4 - %-) N6- NN 4 .4 C.4 0 .4N Ho. up r-( r- N4 Nn NO. N N- NN M 040 H >.A WIx 0 -Cl 4c. q*j r- W W4 en H~C t-4OHI C00 C4 (D V 00 000~0 o CO a ccN H𔃺...I- I-- ə IW C-4 /) I-I. z I.- 2 z 2 Z z 3 IS I We-i if < 00 1-1 0 #-4 1-0 I- 1-4 0- < IS I Web Uo IP- I-- If M&#34 ISO a a a 0 0 a 1-i ,0W UZ IS Z 2w 2...C4 C4 C4 Cj C-4 e4 C4 C4 C,4 cm ൌ (4 cli 04 " cli cm CS1 C4 e4 cm C14 c4cljcqclI"NNe4rqNN CIA CS1 C4 eq C14 C14 C4 C4 N C4 CSI eq C4 1 131 wix

  15. 105KE and 105KW Basins fuel and sludge consolidation study, summary report

    International Nuclear Information System (INIS)

    Gant, R.G.

    1994-01-01

    This study is a summary report that examines and evaluates the feasibility of consolidating irradiated fuel and sludge currently in KE Basin with that in the KW Basin. This study was conducted in support of TPA Milestone (target date) M-34-00-T03. The report summarizes three supporting engineering studies involving: (1) spent fuel consolidation into the single basin, (2) transport of the encapsulated fuel between KE and KW Basins, and (3) dispositioning contaminated water remaining in KE Basin. From the three reports, and preferred storage method, transfer method and water disposition method were defined. These consolidation methods were then evaluated against the no action alternative of continued storage using both KE and KW Basins. The report concluded that the fuel and sludge currently stored in KE Basin not be consolidated in the KW Basin, primarily due to increased cost and radiation exposure required to consolidate the fuel and sludge. Consolidation is more attractive for storage periods beyond the year 2002, which is the study period of the report

  16. Who were the male founders of rural Brazilian Afro-derived communities? A proposal based on three populations.

    Science.gov (United States)

    Ribeiro, Guilherme Galvarros Bueno Lobo; Abe-Sandes, Kiyoko; Barcelos, Rejane da Silva Sena; Klautau-Guimarães, Maria de Nazaré; Junior, Wilson Araujo da Silva; Oliveira, Silviene Fabiana de

    2011-03-01

    Brazilian Quilombos are Afro-derived communities founded mainly by fugitive slaves between the 16(th) and 19(th) centuries; they can be recognized today by ancestral and cultural characteristics. Each of these remnant communities, however, has its own particular history, which includes the migration of non-African derived people. The present work presents a proposal for the origin of the male founder in Brazilian quilombos based on Y-haplogroup distribution. Y haplogroups, based on 16 binary markers (92R7, SRY2627, SRY4064, SRY10831.1 and .2, M2, M3, M09, M34, M60, M89, M213, M216, P2, P3 and YAP), were analysed for 98 DNA samples from genetically unrelated men from three rural Brazilian Afro-derived communities-Mocambo, Rio das Rãs and Kalunga-in order to estimate male geographic origin. Data indicated significant differences among these communities. A high frequency of non-African haplogroups was observed in all communities. This observation suggested an admixture process that has occurred over generations and directional mating between European males and African female slaves that must have occurred on farms before the slaves escaped. This means that the admixture occurred before the slaves escaped and the foundation of the quilombo.

  17. Evaluating the psychometric properties of the Polish version of the Body Appreciation Scale-2.

    Science.gov (United States)

    Razmus, Magdalena; Razmus, Wiktor

    2017-12-01

    This study aimed to investigate the factor structure and psychometric properties of a Polish version of the Body Appreciation Scale-2 (BAS-2; Tylka & Wood-Barcalow, 2015). Data were collected from 721 individuals residing in various regions of Poland. There were two subsamples (n=336, age M=34.95, SD=10.83; and n=385, age M=35.38, SD=10.83). Both principal-axis and confirmatory factor analyses supported the one-dimensional structure of BAS-2 scores. Moreover, full scalar invariance of the BAS-2 in Poland across sex was demonstrated. Scores on the Polish BAS-2 had adequate internal consistency. Convergent validity was demonstrated through significant correlations between BAS-2 scores and variables related to body image (body and appearance self-conscious emotions), well-being (self-esteem, positive affect, and positive orientation), and body mass index. These results indicate that the Polish BAS-2 is an appropriate and psychometrically-sound measure of body appreciation. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. PENGARUH CARA PENGAWETAN TERHADAP KOMPOSISI KIMIA DAN EFISIENSI DALAM BENTUK HAY DAN SILASE PADA DAUN 16 PROVENAN GAMAL (GLIRICIDIA SEPIUM

    Directory of Open Access Journals (Sweden)

    A. W. Puger

    2012-09-01

    Full Text Available RINGKASAN Percobaan yang bertujuan untuk mengetahui pengaruh cara pengawetan terhadap kandungan zat-zat makanan dan efisiensinya pada daun 16 provenan gamal telah dilaksanakan selama 3 bulan. Penelitian menggunakan rancangan acak lengkap yang terdiri atas 3 perlakuan pengawetan (gamal segar, gamal hay, dan gamal silase dan 3 blok sebagai ulangan. Setiap perlakuan terdiri atas 16 provenan, yaitu 6 dari Mexico (M, 4 dari Guatemala (G, dan satu provenan masing-masing dari Colombia (C, Indonesia (I, Nicaragua (N, Panama (P, Costa Rica (R, dan Venezuela (V. Sampel setiap daun (helai dan tangkai provenan sebelum dan setelah diawetkan dianalisis kandungan zat-zat makanannya dan dihitung efisiensinya setelah dibuat hay dan silase. Kandungan DM dan CP dari hay adalah tertinggi (P0,05 setelah diawetkan menjadi hay dan silase, sedangkan efisiensi DM, OM, dan CP dari hay lebih tinggi (P<0,05 daripada silase. Provenan P13, R12, dan M34 mengandung zat-zat makanan lebih tinggi; sementara provenan G14 dan G17 lebih efisien bila diawetkan dalam bentuk hay dan silase. Pada pertanian lahan kering pengawetan gamal dalam bentuk hay lebih efektif dan efisien jika dibandingkan dengan dalam bentuk silase.

  19. Different bacteriocin activities of Streptococcus mutans reflect distinct phylogenetic lineages

    DEFF Research Database (Denmark)

    Balakrishnan, M; Simmonds, RS; Kilian, Mogens

    2002-01-01

    Bacteriocins produced by mutans streptococci are known as mutacins. In this study 16 broadly active mutacin-producing Streptococcus mutans strains from New Zealand, North America and Europe were classified into four groups (A-D) on the basis of differences in their activity in deferred antagonism...... described by Caufield and co-workers. One of the New Zealand isolates of group A (S. mutans strain N) appeared to produce inhibitory activity similar to that of the group I prototype strain UA140. Four other New Zealand isolates of group B (S. mutans strains M19, M34, B34 and D14) had mutacin II......-like activity. The group B mutacin producers differed from the group A mutacin producers in their additional activity against Staph. aureus 46. Seven S. mutans strains (M46, B46, B57, M12, M28, B28 and 13M) were distinguished from the group A and group B mutacin producers in that they inhibited E. faecium TE1...

  20. A Three-Dimensional Transonic, Potential Flow Computer Program, Its Conversion to IBM Fortran and Utilization

    Science.gov (United States)

    1983-12-01

    8217 «-H co«» omro rococo >r>o inr* uoocMcoo^ooin^rmoooroh-ro^cnp-co^cof-cTtro ^Hv»>Ot-»>>»>j"(oromr^>-isOcoooo^H>f coroooococoofoco OX«,<D<yi^’t|Tl...oooooooooooooooooooooo Om*T rococo cMvom-j-votn HVOOOC^OH oucnr-ficM**&#34 >-i<M>rcM>r<J, oo »r o^co oooo r-st sj- oo ^ vO vj» Is» vO o» m ro...I I I I I I I I I I I I I I I I m fM co o CM »-4 co in -4" CM m r-t co o m r* »* <x> o >!• m o OCO^CMCO>f rOCOCO *00>>TOCM’HfO rH^O^rvOCO co,Mcooco

  1. Symbol Stream Combining Versus Baseband Combining for Telemetry Arraying

    Science.gov (United States)

    Divsalar, D.

    1983-01-01

    The objectives of this article are to investigate and analyze the problem of combining symbol streams from many Deep Space Network stations to enhance bit signal-to-noise ratio and to compare the performance of this combining technique with baseband combining. Symbol stream combining (SSC) has some advantages and some disadvantages over baseband combining (BBC). The SSC suffers almost no loss in combining the digital data and no loss due to the transmission of the digital data by microwave links between the stations. The BBC suffers 0.2 dB loss due to alignment and combining the IF signals and 0.2 dB loss due to transmission of signals by microwave links. On the other hand, the losses in the subcarrier demodulation assembly (SDA) and in the symbol synchronization assembly (SSA) for SSC are more than the losses in the SDA and SSA for BBC. It is shown that SSC outperforms BBC by about 0.35 dB (in terms of the required bit energy-to-noise spectral density for a bit error rate of 1,000) for an array of three DSN antennas, namely 64 m, 34m(T/R) and 34m(R).

  2. Affective control and life satisfaction in thalassemics

    Directory of Open Access Journals (Sweden)

    Carmela Mento

    2015-01-01

    Full Text Available Background. Thalassemia is a chronic disease that can lead to an impact on psychological functioning and social behavior of patients. However, still little is known about the specific psychological aspects of the disease, such as the degree of tension, life satisfaction and affective control, especially in adult patients. Aim. The purpose of this study is to investigate whether patients with thalassemia have specific psychological pattern relating to the dimensions of tension, satisfaction and quality of life, management of affection. Method. We evaluated 31 patients with thalassemia major and intermedia (19 women and 12 men aged between 18 and 50 years (M = 34 + 16, belonging to the Complex Unit of Medical Genetics. For the evaluation were used the Profile of Mood States (POMS, the Quality of Life Enjoyment and Satisfaction Questionnaire (Q-LES-Q and the Rorschach test. Results. The findings show an inverse relationship between the levels of self-reported tension and the affective control indicators at Rorschach. Life satisfaction, instead, seems to vary according to the severity of the disease - major vs. intermediate - and the type of therapy. Conclusions. An understanding of the psychological mechanisms involved in thalassemia, both self-reported and projective, can contribute to a wider patient takeover, by considering the subjective aspects related to the psychological and socioemotional well-being, fundamental in the care compliance.

  3. The influence of attraction to partner on heterosexual women's sexual and relationship satisfaction in long-term relationships.

    Science.gov (United States)

    Mark, Kristen P; Herbenick, Debby

    2014-04-01

    Previous research has consistently found that attraction is important in the formation of relationships though research on attraction in long-term relationships is less well understood. This article examined the predictive value of self-reported attraction to partner and change in attraction to partner on sexual and relationship satisfaction in 176 women in committed heterosexual relationships using online survey methodology. Participants' age ranged from 21 to 56 (M = 34.5) years and their relationship length ranged from 5 to 35 (M = 11.75) years. Hierarchical multiple regression results indicated that change in attraction to partner was the most salient predictor of sexual satisfaction, with current attraction to partner also related to women's sexual satisfaction, accounting for 20 % of the variance. Current attraction to partner was the only significant predictor of women's relationship satisfaction, accounting for 22 % of the variance. Additionally, attraction variables accounted for variance above and beyond the impact of relationship and sexual satisfaction. These findings suggest that self-reported attraction to partner is an important contributor to women's satisfaction outcomes in long-term relationships. Further studies in the area of attraction to partner that include couple dynamics and longitudinal data are encouraged and implications for therapists, clinicians, and educators are discussed.

  4. Prediction of Participation of Undergraduate University Students in a Music and Dance Master’s Degree Program

    Directory of Open Access Journals (Sweden)

    Evangelos Bebetsos

    2015-07-01

    Full Text Available The aim of the study was the investigation of students’ attitudes and intention towards their possible participation in a graduate Music and Dance Distance Learning Master’s Degree Program. The sample consisted of consisted of 229 undergraduate University students, between the ages of 20 to 63 yrs. of age (M=34.24, SD=10.70. More specifically, 134 were students of the Hellenic Open University and 95 were students of the School of Physical Education and Sport Science, of the Democritus University of Thrace. The sample completed the version the “Planned Behavior Theory” questionnaire. Results revealed differences among students of both Universities, between experienced and less experienced ones, and also among age groups. On the contrary, no sex differences in any of the questionnaire’s factors were indicated. In conclusion, the findings of this research allow a better understanding of the distance education process, which explains the attitudes and intention(s of students’ participation, and the factors that might influence theirparticular participation.

  5. Religious and Political Conservatism and Beliefs About Same-Sex Parenting in Portugal

    Directory of Open Access Journals (Sweden)

    Pedro Alexandre Costa

    2014-03-01

    Full Text Available AimDuring the last decade, there have been political changes regarding the rights of Lesbian, Gay, Bisexual, and Transgender (LGBT individuals in Portugal, such as the right to marry. However, parenting by same-sex couples is not legally allowed. The purpose of this study was to assess Portuguese heterosexuals’ beliefs about same-sex parenting, and the role of religious and political conservatism in shaping these beliefs.MethodA total of 993 participants, aged between 18 and 69 years (M = 34; SD = 11, responded to one of three questionnaires that included a case vignette depicting a different-sex, a female same-sex, or a male same-sex couple wishing to adopt a child. Participants were then asked to evaluate whether the couple would be suitable to adopt a child, and whether they anticipated any social and emotional problems with the child.ResultsParticipants consistently anticipated more children’s social and emotional problems if they were adopted by a same-sex couple. Men evaluated same-sex couples less favourably than women, and even less so the male same-sex couple.ConclusionIt was found that both religious conservatism and right-wing political leaning were associated with more sexual prejudicial beliefs regarding same-sex couples.

  6. Purpose-in-Life Test: Comparison of the Main Models in Patients with Mental Disorders.

    Science.gov (United States)

    García-Alandete, Joaquín; Marco, José H; Pérez, Sandra

    2017-06-27

    The aim of this study was to compare the main proposed models for the Purpose-In-Life Test, a scale for assessing meaning in life, in 229 Spanish patients with mental disorders (195 females and 34 males, aged 13-68, M = 34.43, SD = 12.19). Confirmatory factor-analytic procedures showed that the original model of the Purpose-In-Life Test, a 20-item unidimensional scale, obtained a better fit than the other analyzed models, SBχ2(df) = 326.27(170), SBχ2/df = 1.92, TLI = .93, CFI = .94, IFI = .94, RMSEA = .063 (90% CI [.053, .074]), CAIC = -767.46, as well as a high internal consistency, (α = .90). The main conclusion is that the original version of the Purpose-In-Life shows a robust construct validity in a clinical population. However, authors recommend an in-depth psychometric analysis of the Purpose-In-Life Test among clinical population. Likewise, the importance of assessing meaning in life in order to enhance psychotherapeutic treatment is noted.

  7. Economic Returns to Military Service

    Science.gov (United States)

    1991-07-01

    0V- 0 M. -qt r-V cto N r- 0M r- . WoN-- W o - %9D I II II II 0 0 N1 LA cc IV V- o InN0 C% ~ Hn Nn m LA 0 N H 0 01 tD No W F-4 cc N 0 C ) ko % A H...A r E o.1 10 C "! o . ;-H 0 M 0 H0(rOlr-4NNII IIZ if ;t, 4 HE-4A c ~ - O IHQ I A H ~ O itII II II --- II II II II e r o o o 1 ’ ’ II o4rII en IM CO O0...a; 1 , 4 c ; A c 8 % W II II - Nr-4 v C-4,r- II 0 11 IfM, 11 W r- tD 0 .11 II II (nI M m &#34:crN o II •H M N W..M-- O M - 4 r-I r4II o i ~N ~ HII II ,l

  8. Repetitive negative thinking predicts depression and anxiety symptom improvement during brief cognitive behavioral therapy.

    Science.gov (United States)

    Kertz, Sarah J; Koran, Jennifer; Stevens, Kimberly T; Björgvinsson, Thröstur

    2015-05-01

    Repetitive negative thinking (RNT) is a common symptom across depression and anxiety disorders and preliminary evidence suggests that decreases in rumination and worry are related to improvement in depression and anxiety symptoms. However, despite its prevalence, relatively little is known about transdiagnostic RNT and its temporal associations with symptom improvement during treatment. The current study was designed to examine the influence of RNT on subsequent depression and anxiety symptoms during treatment. Participants (n = 131; 52% female; 93% White; M = 34.76 years) were patients presenting for treatment in a brief, cognitive behavior therapy based, partial hospitalization program. Participants completed multiple assessments of depression (Center for the Epidemiological Studies of Depression-10 scale), anxiety (the 7-item Generalized Anxiety Disorder Scale), and repetitive negative thinking (Perseverative Thinking Questionnaire) over the course of treatment. Results indicated statistically significant between and within person effects of RNT on depression and anxiety, even after controlling for the effect of time, previous symptom levels, referral source, and treatment length. RNT explained 22% of the unexplained variability in depression scores and 15% of the unexplained variability in anxiety scores beyond that explained by the control variables. RNT may be an important transdiagnostic treatment target for anxiety and depression. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. The Relationship between the Physical Activity Environment, Nature Relatedness, Anxiety, and the Psychological Well-being Benefits of Regular Exercisers

    Science.gov (United States)

    Lawton, Emma; Brymer, Eric; Clough, Peter; Denovan, Andrew

    2017-01-01

    Research from a variety of scientific fields suggests that physical activity in nature and feelings of connection to nature enhance psychological health and well-being. This study investigated the psychological health and well-being impact of the physical activity environment for those already undertaking the recommended weekly amount of physical activity. This topic is important for the design of health and well-being environments and interventions involving physical activity. Participants (N = 262) aged 18–71 years (M = 34.5, SD = 13.1) who met the UK physical activity guidelines completed the Nature Relatedness Scale, the trait section of the State Trait Inventory for Cognitive and Somatic Anxiety and the Psychological Well-Being Scale. Analysis via Multivariate ANOVA indicated that participants who engaged in outdoor physical activity reported significantly lower somatic anxiety levels and higher Nature Relatedness experience (NRexp). Significant results were not evident for wellbeing. Hierarchical regressions revealed that the psychological well-being facet of autonomy, NRexp, and outdoor physical activity predicted lower somatic anxiety, whereas indoor physical activity predicted higher somatic anxiety. Results indicate that somatic anxiety is lower for outdoor physical activity participation, and that outdoor activity, in conjunction with autonomy and NRexp, predicts lower anxiety levels. The findings extend previous work by demonstrating the impact of the physical activity environment on anxiety levels, as well as the contribution of outdoor physical activity and well-being facets to the previously established Nature Relatedness-anxiety relationship. PMID:28694788

  10. Clinical Utility and Psychometric Properties of the Traumatic Brain Injury Quality of Life Scale (TBI-QOL) in US Military Service Members.

    Science.gov (United States)

    Lange, Rael T; Brickell, Tracey A; Bailie, Jason M; Tulsky, David S; French, Louis M

    2016-01-01

    To examine the clinical utility and psychometric properties of the Traumatic Brain Injury Quality of Life (TBI-QOL) scale in a US military population. One hundred fifty-two US military service members (age: M = 34.3, SD = 9.4; 89.5% men) prospectively enrolled from the Walter Reed National Military Medical Center and other nationwide community outreach initiatives. Participants included 99 service members who had sustained a mild traumatic brain injury (TBI) and 53 injured or noninjured controls without TBI (n = 29 and n = 24, respectively). Participants completed the TBI-QOL scale and 5 other behavioral measures, on average, 33.8 months postinjury (SD = 37.9). Fourteen TBI-QOL subscales; Neurobehavioral Symptom Inventory; Posttraumatic Stress Disorder Checklist-Civilian version; Alcohol Use Disorders Identification Test; Combat Exposure Scale. The internal consistency reliability of the TBI-QOL scales ranged from α = .91 to α = .98. The convergent and discriminant validity of the 14 TBI-QOL subscales was high. The mild TBI group had significantly worse scores on 10 of the 14 TBI-QOL subscales than the control group (range, P quality of life in a mild TBI military sample. Additional research is recommended to further evaluate the clinical utility of the TBI-QOL scale in both military and civilian settings.

  11. L’occasione e l’eterno: la tenda di Tolomeo Filadelfo nei palazzi di Alessandria. Parte seconda. Una proposta di ricostruzione

    Directory of Open Access Journals (Sweden)

    Elena Calandra

    2009-05-01

    Full Text Available The work is the second part of an essay published in “Lanx” 1 (2008.After exposing the previous reconstructions, a new proposal is advanced, which explains the marquee through the macedonian architecture and suggests a new architectonical building type, the oecus aegyptius. Since only the interior is known, the dimensions are calculated (m 34 x 26 approximately for the base, m 22 for the height of the columns, with the addition of the roof, and the constitutive elements are interpreted according to the comparison with the archaeological and literary evidence: the palm- and thyrsos-shaped columns, the nymphai and the antra, the statue bases, the arch door, the lacunar ceiling, the sloping roof, the furniture, the gold and silver vases, the works of art. The exhibition lasts one year, and consequently becomes a new “event”, in connection with the contemporary dionysiac pompé: the works of art acquire a new meaning, connoting the Lagides as refined collectors, and are part of a cultural and political program, probably organized by the Museum scholars. The exhibition, indeed, is marked by an encyclopaedical purpose: on one hand it seems to be the ephemeral equivalent of the  Museum and of the zoo promoted  by Ptolemy I, on the other it takes part in the cultural construction of the Ptolemean family, being almost its dynastic monument.

  12. Airport Activity Statistics of Certificated Route Air Carriers.

    Science.gov (United States)

    1982-12-31

    BY CDVAM. AND oY cAm 12 NWTHS EMo OECIoAER 11, 1912 Typw. " FbW w (I -- pim )a~t W . 0 Hub lm .d pIt of mnl-tsn-d I "EATER BUFFALa INTIRMA0IONAL...1419 4 Ott .3A) -SC’"J0’ 9.󈧭 6)6). j.4, )7t𔄀 1~ 76.7 .6 0700 44 ’ e--.0) 2 34 15.2 49’ t.)9ඦ 41.) .5 1~.0 .4 21.4 3 * 8)) T9 1, .9C 4 3,. 1.) ll...IT C0*8830~ AM 87 C8MMM 32 8838.38 18180u ott .349. i1. 348.. A-. . I C...m&#34 I 3 4 1 A- *3 Sf 33----SL3H2UL- 1,14.. 732 .4 8613 2.2 760 16 AS JU 24333

  13. Molecular epidemiology of DFNB1 deafness in France

    Directory of Open Access Journals (Sweden)

    Molinari Nicolas

    2004-03-01

    Full Text Available Abstract Background Mutations in the GJB2 gene have been established as a major cause of inherited non syndromic deafness in different populations. A high number of sequence variations have been described in the GJB2 gene and the associated pathogenic effects are not always clearly established. The prevalence of a number of mutations is known to be population specific, and therefore population specific testing should be a prerequisite step when molecular diagnosis is offered. Moreover, population studies are needed to determine the contribution of GJB2 variants to deafness. We present our findings from the molecular diagnostic screening of the GJB2 and GJB6 genes over a three year period, together with a population-based study of GJB2 variants. Methods and results Molecular studies were performed using denaturing High Performance Liquid Chromatograghy (DHPLC and sequencing of the GJB2 gene. Over the last 3 years we have studied 159 families presenting sensorineural hearing loss, including 84 with non syndromic, stable, bilateral deafness. Thirty families were genotyped with causative mutations. In parallel, we have performed a molecular epidemiology study on more than 3000 dried blood spots and established the frequency of the GJB2 variants in our population. Finally, we have compared the prevalence of the variants in the hearing impaired population with the general population. Conclusion Although a high heterogeneity of sequence variation was observed in patients and controls, the 35delG mutation remains the most common pathogenic mutation in our population. Genetic counseling is dependent on the knowledge of the pathogenicity of the mutations and remains difficult in a number of cases. By comparing the sequence variations observed in hearing impaired patients with those sequence variants observed in general population, from the same ethnic background, we show that the M34T, V37I and R127H variants can not be responsible for profound or severe

  14. Drug dependence and psychological distress in Portuguese patients entering a substance abuse treatment center

    Directory of Open Access Journals (Sweden)

    Paulo P. P. Machado

    2007-01-01

    Full Text Available En el contexto de la situación actual de los programas de tratamiento de las toxiocodependencias, las características psicopatológicas parecen estar asociadas con resultados terapéuticos negativos. El abuso de drogas, en especial la dependencia, es una perturbación compleja que incluye síntomas cognitivos, comportamentales y fisiológicos. El amplio espectro de síntomas que caracterizan estas perturbaciones es responsable de su heterogeneidad clínica en términos de manifestación clínica, severidad, vulnerabilidad, secuelas y comorbilidad que por sí solo implica un elevado sufrimiento y necesidad de intervención en diferentes áreas. La evolución de la toxicodependencia y de la psicopatología se constituye como un gran desafío para los profesionales que trabajan en estos contextos. El presente estudio descriptivo trata de explorar algunas características principales de los individuos con problemas de toxicodependencia que iniciaron un programa de tratamiento. Participaron en el estudio 107 sujetos, con edades comprendidas entre 22 y 55 años (M = 34,84; DT = 6,44, que frecuentan un centro metropolitano de tratamiento de las toxicodependencias, 90,7% de los cuales pertenecen al sexo masculino. Los resultados señalan la presencia de algunas relaciones entre la severidad del consumo y la sintomatología psicopatológica.

  15. The representation of love among Brazilians, Russians and Central Africans: A comparative analysis.

    Directory of Open Access Journals (Sweden)

    Koyanongo E.

    2016-05-01

    Full Text Available This paper is dedicated to the cultural specificities of three typical collective groups with respect to the representation of love. The research subject focuses on the cross-cultural similarities and differences in how love is conceptualized among highly educated citizens of Brazil (50, Russia (50, and Central Africa (50 (age range 21–60; M = 34. We used “The Classical ideas of love: acceptance and distancing” questionnaire (I.A. Djidaryan, E.V. Belovol, & O.V. Maslova and the “Directed associations with ‘love’ as the wordstimulus” technique (on the basis of C.G. Jung’s associative experiment and P. Vergès’s methodology. The results show similarities and differences in how love is represented among the groups. The following similarities were found: Love is seen as all that is good and kind about a person, a way to become better. At the peripheral level, the social representation of love includes friendship, patience, and passion. At the point of cross-cultural differences, it was found that: a The main emotion reflecting how love is represented for Brazilians is honesty, for Russians — suffering, for Central Africans — tenderness; b Brazilians understand love as a sensual, personal moral choice; Russians perceive love as an obstacle, a problem in itself; Central Africans conceptualize love as God-given and ennobling of the person; c love is conceptualized as something inherent and family-oriented among Russians, intrapersonal and intimate among Brazilians, and divine among Central Africans. The results mean that within peripheral confines, the notion of love among the groups matches to a certain extent R. Sternberg’s triangle of love, while its core zone is culturally specific.

  16. Fatty acid CoA ligase-4 gene polymorphism influences fatty acid metabolism in metabolic syndrome, but not in depression.

    Science.gov (United States)

    Zeman, Miroslav; Vecka, Marek; Jáchymová, Marie; Jirák, Roman; Tvrzická, Eva; Stanková, Barbora; Zák, Ales

    2009-04-01

    The composition of polyunsaturated fatty acids (PUFAs) in cell membranes and body tissues is altered in metabolic syndrome (MetS) and depressive disorder (DD). Within the cell, fatty acid coenzyme A (CoA) ligases (FACLs) activate PUFAs by esterifying with CoA. The FACL4 isoform prefers PUFAs (arachidonic and eicosapentaenoic acid) as substrates, and the FACL4 gene is mapped to Xq23. We have analyzed the association between the common single nucleotide polymorphism (SNP) (rs1324805, C to T substitution) in the first intron of the FACL4 gene and MetS or DD. The study included 113 healthy subjects (54 Males/59 Females), 56 MetS patients (34M/22F) and 41 DD patients (7M/34F). In MetS group, T-carriers and patients with CC or C0 (CC/C0) genotype did not differ in the values of metabolic indices of MetS and M/F ratio. Nevertheless, in comparison with CC/C0, the T-allele carriers were characterized by enhanced unfavorable changes in fatty acid metabolism typical for MetS: higher content of dihomogammalinolenic acid (P phosphatidylcholine (PC) (P = 0.052), lower index of Delta5 desaturation (P insulin, conjugated dienes and index of insulin resistance, but showed no significant association with the studied SNP. The present study shows that the common SNP (C to T substitution) in the first intron of the FACL4 gene is associated with altered FA composition of plasma phosphatidylcholines in patients with MetS.

  17. Stability of Mental Toughness, Sleep Disturbances, and Physical Activity in Patients With Multiple Sclerosis (MS—A Longitudinal and Pilot Study

    Directory of Open Access Journals (Sweden)

    Dena Sadeghi Bahmani

    2018-05-01

    Full Text Available Background: Previous research of patients with multiple sclerosis (MS focused prevalently on fatigue, depression, and cognitive dysfunction during the clinical course. By contrast, research on the longer-term characteristics of physical activity (PA, psychological functioning, and sleep problems is scarce. The aims of the present study were therefore to examine changes in PA, mental toughness (MT as a proxy of psychological functioning, and sleep disturbances over a 2-year period of time after disease onset.Methods: A total of 18 patients with diagnosed MS (mean age: M = 34.29 years took part in this longitudinal study. First, 1–4 weeks after the first symptoms, a neurologist diagnosed the MS. Second, they completed a series of questionnaires covering socio-demographic data, PA, MT, and sleep disturbances. Third, the same questionnaires were completed again 2 years later (follow-up. Last, a neurologist assessed the degree of disability with the Expanded Disability Status Scale (EDSS.Results: Two years after MS onset, patients had lower levels of vigorous PA, but no statistically significant changes in moderate PA were observed. Further, walking time increased and sedentary time decreased. Patients with sleep disturbances at disease onset also reported poor sleep 2 years later. MT scores remained stable over time. EDSS scores worsened, though, change in EDSS was not associated with PA, MT, or sleep.Conclusions: Two years after disease onset, patients with MS reported similar MT levels and sleep disturbances. PA shifted from vigorous PA toward walking and a less sedentary lifestyle, while moderate PA remained unchanged. The pattern of results of the present pilot study suggests that at the early stage of the MS course, there is no obstacle for being physically active, nor did sleep and MT as a proxy of psychological functioning decrease in a substantial way.

  18. Thermal screening of facial skin arterial hot spots using non-contact infrared radiometry

    International Nuclear Information System (INIS)

    Williams, E M; Heusch, A I; McCarthy, P W

    2008-01-01

    Non-contact infrared thermometry of facial skin offers advantages over less accessible internal body sites, especially when considering mass screening for febrile infectious disease. The forehead offers an obvious site, but does not present an isothermic surface, as various small arteries passing close to the surface create 'hot-spots'. The aim of this study is to use non-contact infrared (IR) thermometry to determine the link between the temperature at specific facial skin sites and clinical body temperature. A sample of 169 asymptomatic adults (age range 18–54 years) was screened with IR thermometers (Braun Thermoscan proLT for auditory meatus (AM) temperature representing clinical body temperature, and a Raytek, Raynger MX for skin surface temperature). Peak IR skin temperature was measured over the course of each posterior auricular artery (PAA) and each superficial temporal artery (STA). In a sub-group (n = 54) the peak skin temperature of the forehead's metopic region (MR) was also recorded. There were no differences (P > 0.05) between the PAA and STA at 34.2 ± 0.9 °C and 34.2 ± 0.7 °C, respectively, which were 2.5 °C cooler than the AM temperature (36.7 ± 0.5 °C, p 2 = 0.63, p < 0.001) between PAA and STA. There were no asymmetric temperature differences between the left and right sides and males had warmer skin over the MR (F, 33.6 ± 0.7 °C versus M, 34.4 ± 0.6 °C, p < 0.001). Although a lack of correlation between either PAA or STA and AM was apparent in asymptomatics, further research in symptomatics is required to determine the usefulness of these measurements in mass screening of conditions such as fever

  19. Strategies to distinguish new synthetic cannabinoid FUBIMINA (BIM-2201) intake from its isomer THJ-2201: metabolism of FUBIMINA in human hepatocytes.

    Science.gov (United States)

    Diao, Xingxing; Scheidweiler, Karl B; Wohlfarth, Ariane; Zhu, Mingshe; Pang, Shaokun; Huestis, Marilyn A

    Since 2013, a new drugs-of-abuse trend attempts to bypass drug legislation by marketing isomers of scheduled synthetic cannabinoids (SCs), e.g., FUBIMINA (BIM-2201) and THJ-2201. It is much more challenging to confirm a specific isomer's intake and distinguish it from its structural analog because the isomers and their major metabolites usually have identical molecular weights and display the same product ions. Here, we investigated isomers FUBIMINA and THJ-2201 and propose strategies to distinguish their consumption. THJ-2201 was scheduled in the US, Japan, and Europe; however, FUBIMINA is easily available on the Internet. We previously investigated THJ-2201 metabolism in human hepatocytes, but human FUBIMINA metabolism is unknown. We aim to characterize FUBIMINA metabolism in human hepatocytes, recommend optimal metabolites to confirm its consumption, and propose strategies to distinguish between intakes of FUBIMINA and THJ-2201. FUBIMINA (10 μM) was incubated in human hepatocytes for 3 h, and metabolites were characterized with high-resolution mass spectrometry (HR-MS). We identified 35 metabolites generated by oxidative defluorination, further carboxylation, hydroxylation, dihydrodiol formation, glucuronidation, and their combinations. We recommend 5'-OH-BIM-018 (M34), BIM-018 pentanoic acid (M33), and BIM-018 pentanoic acid dihydrodiol (M7) as FUBIMINA specific metabolites. THJ-2201 produced specific metabolite markers 5'-OH-THJ-018 (F26), THJ-018 pentanoic acid (F25), and hydroxylated THJ-2201 (F13). Optimized chromatographic conditions to achieve different retention times and careful selection of specific product ion spectra enabled differentiation of isomeric metabolites, in this case FUBIMINA from THJ-2201. Our HR-MS approach should be applicable for differentiating future isomeric SCs, which is especially important when different isomers have different legal status.

  20. Resting energy expenditure prediction in recreational athletes of 18-35 years: confirmation of Cunningham equation and an improved weight-based alternative.

    Science.gov (United States)

    ten Haaf, Twan; Weijs, Peter J M

    2014-01-01

    Resting energy expenditure (REE) is expected to be higher in athletes because of their relatively high fat free mass (FFM). Therefore, REE predictive equation for recreational athletes may be required. The aim of this study was to validate existing REE predictive equations and to develop a new recreational athlete specific equation. 90 (53 M, 37 F) adult athletes, exercising on average 9.1 ± 5.0 hours a week and 5.0 ± 1.8 times a week, were included. REE was measured using indirect calorimetry (Vmax Encore n29), FFM and FM were measured using air displacement plethysmography. Multiple linear regression analysis was used to develop a new FFM-based and weight-based REE predictive equation. The percentage accurate predictions (within 10% of measured REE), percentage bias, root mean square error and limits of agreement were calculated. Results: The Cunningham equation and the new weight-based equation REE(kJ / d) = 49.940* weight(kg) + 2459.053* height(m) - 34.014* age(y) + 799.257* sex(M = 1,F = 0) + 122.502 and the new FFM-based equation REE(kJ / d) = 95.272*FFM(kg) + 2026.161 performed equally well. De Lorenzo's equation predicted REE less accurate, but better than the other generally used REE predictive equations. Harris-Benedict, WHO, Schofield, Mifflin and Owen all showed less than 50% accuracy. For a population of (Dutch) recreational athletes, the REE can accurately be predicted with the existing Cunningham equation. Since body composition measurement is not always possible, and other generally used equations fail, the new weight-based equation is advised for use in sports nutrition.

  1. Substance abuse and criminal thinking: testing the countervailing, mediation, and specificity hypotheses.

    Science.gov (United States)

    Walters, Glenn D

    2012-12-01

    The purpose of this study was to determine (a) which of 2 dimensions of criminal thinking (proactive and/or reactive) correlates with prior substance abuse; (b) whether criminal thinking mediates the relationship between prior substance abuse and recidivism; (c) if a direct relationship exists between specific drugs of abuse and specific criminal thinking styles. First, the reconstructed Proactive (Prc) and Reactive (Rrc) Criminal Thinking scores from the Psychological Inventory of Criminal Thinking Styles (PICTS; Walters, 1995) were correlated with a dichotomous measure of prior substance abuse and a continuous measure of the number of substances abused in a sample of 2877 male federal prisoners (age: M = 34.96, SD = 9.89, range = 18-84; race: 63.6% Black, 17.3% White, 17.6% Hispanic, 1.4% other). The results indicated that only the Rrc score correlated significantly with prior substance abuse when the effect of the alternative measure (Prc in the case of Rrc and Rrc in the case of the Prc) was controlled through partial correlations. Second, reactive criminal thinking was found to mediate the relationship between a history of prior substance abuse and subsequent recidivism in a subsample of 1101 inmates who were released from prison during a 1- to 76-month follow-up. Third, both specific (alcohol with cutoff; marijuana with cognitive indolence) and global (heroin, cocaine, and amphetamine with cutoff, cognitive indolence, and discontinuity) drug-criminal thinking correlations were obtained. These results suggest that reactive criminal thinking plays a potentially important role in the drug-crime relationship.

  2. Assimilation or integration: Similarities and differences between acculturation attitudes of migrants from Central Asia and Russians in Central Russia.

    Directory of Open Access Journals (Sweden)

    Lebedeva N. M.

    2016-05-01

    Full Text Available This paper is dedicated to the cultural specificities of three typical collective groups with respect to the representation of love. The research subject focuses on the cross-cultural similarities and differences in how love is conceptualized among highly educated citizens of Brazil (50, Russia (50, and Central Africa (50 (age range 21–60; M = 34. We used “The Classical ideas of love: acceptance and distancing” questionnaire (I.A. Djidaryan, E.V. Belovol, & O.V. Maslova and the “Directed associations with ‘love’ as the wordstimulus” technique (on the basis of C.G. Jung’s associative experiment and P. Vergès’s methodology. The results show similarities and differences in how love is represented among the groups. The following similarities were found: Love is seen as all that is good and kind about a person, a way to become better. At the peripheral level, the social representation of love includes friendship, patience, and passion. At the point of cross-cultural differences, it was found that: a The main emotion reflecting how love is represented for Brazilians is honesty, for Russians — suffering, for Central Africans — tenderness; b Brazilians understand love as a sensual, personal moral choice; Russians perceive love as an obstacle, a problem in itself; Central Africans conceptualize love as God-given and ennobling of the person; c love is conceptualized as something inherent and family-oriented among Russians, intrapersonal and intimate among Brazilians, and divine among Central Africans. The results mean that within peripheral confines, the notion of love among the groups matches to a certain extent R. Sternberg’s triangle of love, while its core zone is culturally specific.

  3. Time Delay Mechanical-noise Cancellation (TDMC) to Provide Order of Magnitude Improvements in Radio Science Observations

    Science.gov (United States)

    Atkinson, D. H.; Babuscia, A.; Lazio, J.; Asmar, S.

    2017-12-01

    Many Radio Science investigations, including the determinations of planetary masses, measurements of planetary atmospheres, studies of the solar wind, and solar system tests of relativistic gravity, rely heavily on precision Doppler tracking. Recent and currently proposed missions such as VERITAS, Bepi Colombo, Juno have shown that the largest error source in the precision Doppler tracking data is noise in the Doppler system. This noise is attributed to un-modeled motions of the ground antenna's phase center and is commonly referred to as "antenna mechanical noise." Attempting to reduce this mechanical noise has proven difficult since the deep space communications antennas utilize large steel structures that are already optimized for mechanical stability. Armstrong et al. (2008) have demonstrated the Time Delay Mechanical-noise Cancellation (TDMC) concept using Goldstone DSN antennas (70 m & 34 m) and the Cassinispacecraft to show that the mechanical noise of the 70 m antenna could be suppressed when two-way Doppler tracking from the 70 m antenna and the receive-only Doppler data from the smaller, stiffer 34 m antenna were combined with suitable delays. The proof-of-concept confirmed that the mechanical noise in the final Doppler observable was reduced to that of the stiffer, more stable antenna. Caltech's Owens Valley Radio Observatory (OVRO) near Bishop, CA now has six 10.4 m diameter antennas, a consequence of the closure of Combined Array for Research in Millimeter Astronomy (CARMA). In principle, a 10 m antenna can lead to an order-of-magnitude improvement for the mechanical noise correction, as the smaller dish offers better mechanical stability compared to a DSN 34-m antenna. These antennas also have existing Ka-band receiving systems, and preliminary discussions with the OVRO staff suggest that much of the existing signal path could be used for Radio Science observations.

  4. Fisetin Protects DNA Against Oxidative Damage and Its Possible Mechanism.

    Science.gov (United States)

    Wang, Tingting; Lin, Huajuan; Tu, Qian; Liu, Jingjing; Li, Xican

    2016-06-01

    The paper tries to assess the protective effect of fisetin against •OH-induced DNA damage, then to investigate the possible mechanism. The protective effect was evaluated based on the content of malondialdehyde (MDA). The possible mechanism was analyzed using various antioxidant methods in vitro, including •OH scavenging (deoxyribose degradation), •O2 (-) scavenging (pyrogallol autoxidation), DPPH• scavenging, ABTS•(+) scavenging, and Cu(2+)-reducing power assays. Fisetin increased dose-dependently its protective percentages against •OH-induced DNA damage (IC50 value =1535.00±29.60 µM). It also increased its radical-scavenging percentages in a dose-dependent manner in various antioxidants assays. Its IC50 values in •OH scavenging, •O2(-) scavenging, DPPH• scavenging, ABTS•(+) scavenging, and Cu(2+)-reducing power assays, were 47.41±4.50 µM, 34.05±0.87 µM, 9.69±0.53 µM, 2.43±0.14 µM, and 1.49±0.16 µM, respectively. Fisetin can effectively protect DNA against •OH-induced oxidative damage possibly via reactive oxygen species (ROS) scavenging approach, which is assumed to be hydrogen atom (H•) and/or single electron (e) donation (HAT/SET) pathways. In the HAT pathway, the 3',4'-dihydroxyl moiety in B ring of fisetin is thought to play an important role, because it can be ultimately oxidized to a stable ortho-benzoquinone form.

  5. Oxidative Damage and Its Possible Mechanism

    Directory of Open Access Journals (Sweden)

    Tingting Wang

    2016-06-01

    Full Text Available Purpose: The paper tries to assess the protective effect of fisetin against •OH-induced DNAdamage, then to investigate the possible mechanism.Methods: The protective effect was evaluated based on the content of malondialdehyde(MDA. The possible mechanism was analyzed using various antioxidant methods in vitro,including •OH scavenging (deoxyribose degradation, •O2- scavenging (pyrogallolautoxidation, DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays.Results: Fisetin increased dose-dependently its protective percentages against •OH-inducedDNA damage (IC50 value =1535.00±29.60 μM. It also increased its radical-scavengingpercentages in a dose-dependent manner in various antioxidants assays. Its IC50 values in•OH scavenging, •O2- scavenging, DPPH• scavenging, ABTS•+ scavenging, and Cu2+-reducing power assays, were 47.41±4.50 μM, 34.05±0.87 μM, 9.69±0.53 μM, 2.43±0.14μM, and 1.49±0.16 μM, respectively.Conclusion: Fisetin can effectively protect DNA against •OH-induced oxidative damagepossibly via reactive oxygen species (ROS scavenging approach, which is assumed to behydrogen atom (H• and/or single electron (e donation (HAT/SET pathways. In the HATpathway, the 3’,4’-dihydroxyl moiety in B ring of fisetin is thought to play an importantrole, because it can be ultimately oxidized to a stable ortho-benzoquinone form.

  6. Evaluation of Vitronectin Expression in Prostate Cancer and the Clinical Significance of the Association of Vitronectin Expression with Prostate Specific Antigen in Detecting Prostate Cancer.

    Science.gov (United States)

    Niu, Yue; Zhang, Ling; Bi, Xing; Yuan, Shuai; Chen, Peng

    2016-03-05

    To detect the expression of vitronectin (VTN) in the tissues and blood serum of prostate cancer (PCa) patients, and evaluate its clinical significance and to evaluate the significance of the combined assay of VTN and prostate specific antigens (PSA) in PCa diagnosis. To detect the expression of VTN as a potential marker for PCa diagnosis and prognosis, immunohistochemistry was performed on the tissues of 32 patients with metastatic PCa (PCaM), 34 patients with PCa without metastasis (PCa), and 41 patients with benign prostatic hyperplasia (BPH). The sera were then subjected to Western blot analysis. All cases were subsequently examined to determine the concentrations of PSA and VTN in the sera. The collected data were collated and analyzed. The positive expression rates of VTN in the tissues of the BPH and PCa groups (including PCa and PCaM groups) were 75.61% and 45.45%, respectively (P = .005). VTN was more highly expressed in the sera of the BPH patients (0.83 ± 0.07) than in the sera of the PCa patients (0.65 ± 0.06) (P < .05). It was also more highly expressed in the sera of the PCa patients than in the sera of the PCaM patients (0.35 ± 0.08) (P < .05). In the diagnosis of BPH and PCa, the Youden indexes of PSA detection, VTN detection, and combined detection were 0.2620, 0.3468, and 0.5635; the kappa values were 0.338, 0.304, and 0.448, respectively, and the areas under the receiver operating characteristic curve were 0.625, 0.673, and 0.703 (P < .05), respectively. VTN levels in sera may be used as a potential marker of PCa for the diagnosis and assessment of disease progression and metastasis. The combined detection of VTN and PSA in sera can be clinically applied in PCa diagnosis. .

  7. Educação a Distância - tradução, adaptação e validação da escala de motivação EMITICE

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    Patricia Jantsch Fiuza

    Full Text Available A motivação interfere na forma das pessoas interagirem e nas relações com outros, destaca-se neste estudo a motivação no uso de TIC na educação. Este artigo relata a tradução, adaptação e validação da Escala EMITICE, baseada na Teoria da Autodeterminação (TAD. Realizaram-se duas fases: (1 tradução, adaptação e teste piloto (N=91, (2 teste de campo e validação (N=466 em alunos brasileiros de cursos a distância, idades entre 18 a 61 anos (M=34,48; DP=9,56, 171 homens (36,7% e 295 mulheres (63,3%. Utilizaram-se juízes bilíngues, síntese das traduções, comitê de especialistas e backtranslation. Correlação de Pearson, análise fatorial confirmatória e consistência interna demonstram que a EMITICE é válida e fidedigna. A AFC apresentou adequação ao modelo e boa consistência interna na amostra (α = 0,84. Os resultados reforçam as qualidades psicométricas, indicando aplicabilidade em estudos sobre motivação em relação às TIC, constituindo-se num instrumento teórica e empiricamente embasado, útil à pesquisa científica.

  8. Stellar activity with LAMOST - II. Chromospheric activity in open clusters

    Science.gov (United States)

    Fang, Xiang-Song; Zhao, Gang; Zhao, Jing-Kun; Bharat Kumar, Yerra

    2018-05-01

    We use the LAMOST spectra of member stars in Pleiades, M34, Praesepe, and Hyades to study how chromospheric activity varies as a function of mass and rotation at different age. We measured excess equivalent widths of H α, H β, and Ca II K based on estimated chromospheric contributions from old and inactive field dwarfs, and excess luminosities are obtained by normalizing bolometric luminosity, for more than 700 late-type stars in these open clusters. Results indicate two activity sequences in cool spot coverage and H α excess emission among GK dwarfs in Pleiades and M dwarfs in Praesepe and Hyades, paralleling with well-known rotation sequences. A weak dependence of chromospheric emission on rotation exists among ultrafast rotators in saturated regime with Rossby number Ro ≲ 0.1. In the unsaturated regime, chromospheric and coronal emission show similar dependence on Ro, but with a shift towards larger Ro, indicating chromospheric emission gets easily saturated than coronal emission, and/or convective turnover time-scales based on X-ray data do not work well with chromospheric emission. More interestingly, our analysis shows fully convective slow rotators obey the rotation-chromospheric activity relation similar to hotter stars, confirming the previous finding. We found correlations among H α, H β, and Ca II K emissions, in which H α losses are more important than Ca II K for cooler and more active stars. In addition, a weak correlation is seen between chromospheric emission and photospheric activity that shows dependence on stellar spectral type and activity level, which provides some clues on how spot configuration varies as a function of mass and activity level.

  9. Yersinia enterocolitica and Photorhabdus asymbiotica β-lactamases BlaA are exported by the twin-arginine translocation pathway.

    Science.gov (United States)

    Schriefer, Eva-Maria; Hoffmann-Thoms, Stephanie; Schmid, Franz X; Schmid, Annika; Heesemann, Jürgen

    2013-01-01

    In general, β-lactamases of medically important Gram-negative bacteria are Sec-dependently translocated into the periplasm. In contrast, β-lactamases of Mycobacteria spp. (BlaC, BlaS) and the Gram-negative environmental bacteria Stenotrophomonas maltophilia (L2) and Xanthomonas campestris (Bla(XCC-1)) have been reported to be secreted by the twin-arginine translocation (Tat) system. Yersinia enterocolitica carries 2 distinct β-lactamase genes (blaA and blaB) encoding BlaA(Ye) and the AmpC-like β-lactamase BlaB, respectively. By using the software PRED-TAT for prediction and discrimination of Sec from Tat signal peptides, we identified a functional Tat signal sequence for Yersinia BlaA(Ye). The Tat-dependent translocation of BlaA(Ye) could be clearly demonstrated by using a Y. enterocolitica tatC-mutant and cell fractionation. Moreover, we could demonstrate a unique unusual temperature-dependent activity profile of BlaA(Ye) ranging from 15 to 60 °C and a high 'melting temperature' (T(M)=44.3°) in comparison to the related Sec-dependent β-lactamase TEM-1 (20-50°C, T(M)=34.9 °C). Strikingly, the blaA gene of Y. enterocolitica is present in diverse environmental Yersinia spp. and a blaA homolog gene could be identified in the closely related Photorhabdus asymbiotica (BlaA(Pa); 69% identity to BlaA(Ye)). For BlaA(Pa) of P. asymbiotica, we could also demonstrate Tat-dependent secretion. These results suggest that Yersinia BlaA-related β-lactamases may be the prototype of a large Tat-dependent β-lactamase family, which originated from environmental bacteria. Copyright © 2012 Elsevier GmbH. All rights reserved.

  10. Estudo morfológico do sistema respiratório de quati (Nasua nasua

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    vanessa cristina oliveira

    2011-11-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2012v25n1p81 O objetivo deste trabalho foi descrever a morfologia macroscópica e microscopia de luz dos órgãos respiratórios do quati (Nasua nasua. Utilizou-se cinco animais provenientes do Criatório Científico CECRIMPAS (UNIfeob, os quais foram eutanasiados e fixados em solução de formaldeído a 10% para dissecação, análise e mensuração. Para microscopia de luz, os fragmentos dos órgãos respiratórios, foram processados conforme técnicas rotineiras para histologia e corados por Hematoxilina, Eosina e Azul de Toluidina. O nariz do quati está localizado em plano nasal, formato pontiagudo e voltado para cima, possui a concha etmoidal,/nasal dorsal e nasal ventral separadas pelos meatos: nasal dorsal e ventral. A laringe /possui as quatro cartilagens: aritenóide, cricoíde, epiglote e tireóide e a traqueia contém 34 anéis traqueais separados pelo ligamento traqueal, sendo internamente revestida pelo tecido epitelial pseudoestratificado ciliado. Os pulmões estão subdivididos em lobos por fissuras interlobares, o pulmão direito é dividido em quatro lobos e o esquerdo em dois, sendo este menor que o direito. Microscópicamente os brônquios primários, secundários e terciários possuem a mesma constituição de revestimento interno da traqueia. Concluímos que o aparelho respiratório do quati se assemelha aos achados descritos na literatura para os carnívoros domésticos.

  11. Trauma Related Guilt Inventory - psychometric properties of the Polish adaptation (TRGI-PL).

    Science.gov (United States)

    Popiel, Agnieszka; Zawadzki, Bogdan

    2015-01-01

    AIM : Although various aspects of guilt are frequent problems of patients suffering from PTSD, they have been included into the diagnostic criteria for PTSD just in the present version DSM-5. Kubany proposed a cognitive conceptualization of guilt in PTSD followed by development of the Trauma Related Guilt Inventory (TRGI). The aim of the paper is to present psychometric properties of the Polish version of the inventory - the TRGI-PL. A Polish adaptation of the Trauma-Related Guilt Inventory was applied to a sample of 280 motor vehicle (MVA) participants (147 females, 133 males of age from 18 to 80 (M=34,93, SD=13,71) within 1-24 months after a MVA (M=10,18, SD=6,23). Validation of the Polish version was done by analyzing the internal structure of the instrument and comparing the emotional and cognitive aspects of guilt assessed by the TRGI with PTSD symptoms, post-traumatic cognitions and responsibility for MVA and subjective agreement with the judgment. The model with four latent factors: Distress, Hindsight-Bias/Responsibility, Wrongdoing and Insufficient Justification scales showed acceptable fit (Satorra-Bentler chi2=518,62, df=203, pguilt, obtained in the studies on original TRGI version. Reliability coefficients are similar to original version. Correlations with other PTSD measures showed satisfactory convergent and discriminative validity. The Polish adaptation of the Trauma-Related Guilt Inventory is a reliable and valid tool for assessing guilt as a multidimensional phenomenon, comprising emotional and several cognitive characteristics, in trauma survivors.

  12. A comparative study of the effect of icodextrin based peritoneal dialysis and hemodialysis on lipid metabolism.

    Science.gov (United States)

    Kadiroğlu, A K; Ustündag, S; Kayabaşi, H; Yilmaz, Z; Yildirım, Y; Sen, S; Yilmaz, M E

    2013-09-01

    Dyslipidemia is frequent in patients with end stage renal disease. Excessive peritoneal glucose absorption from high glucose-containing peritoneal dialysis solutions may enhance disturbances on the lipid metabolism of patients on peritoneal dialysis. We compared the effect of icodextrin-based peritoneal dialysis therapy with hemodialysis (HD) therapy on lipid metabolism. A total of 157 non-diabetic patients on dialysis at least for 3 months; 78 patients on Icodextrin-based continuous ambulatory peritoneal dialysis (CAPD) (44 M, 34 F) and 79 patients in HD group (47M, 32F) were included into the study. After 12 h of fasting and before the dialysis session, serum urea, creatinin, glucose, Sodium, potasium, and albumin, total cholesterol (TC), triglycerides (TG), very low density lipoprotein (VLDL), low density lipoprotein (LDL)-C, high-density lipoprotein (HDL)-C, apolipoprotein A (Apo A), apolipoprotein B, and lipoprotein a were measured. TG (P = 0018) and VLDL (P = 0.022) were lower in CAPD group than HD group, HDL-C (P < 0.001) and Apo A (P = 0.001) were higher in CAPD group than in HD group. A total of 24.4% in CAPD group and 11.4% in HD group (P < 0.034) had normal serum levels of TG, LDL-C, and HDL-C. More patients in CAPD group (47.4%) had high serum Apo A levels than in HD group (21.5%) (P = 0.001). We suggest that patients receiving icodextrin-based CAPD may have better TG, HDL-C, and Apo A levels than patients on HD.

  13. Are older people more vulnerable to long-term impacts of disasters?

    Directory of Open Access Journals (Sweden)

    Rafiey H

    2016-12-01

    Full Text Available Hassan Rafiey,1 Yadollah Abolfathi Momtaz,2,3 Fardin Alipour,1 Hamidreza Khankeh,4 Shokoufeh Ahmadi,4 Mohammad Sabzi Khoshnami,1 Sharifah Azizah Haron3 1Research Center of Social Welfare Management, Department of Social Work, 2Iranian Research Center on Aging, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran; 3Malaysian Research Institute on Ageing (MyAgeing, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 4Department of Health in Emergency and Disaster, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran Background: Despite the growing interest in the study of disasters, there is limited research addressing the elderly population that lead to prejudiced beliefs that older adults are more vulnerable to disasters than younger adults. This study aimed to compare positive mental health between elderly and young earthquake survivors.Method: Data for this study, consisting of 324 earthquake survivors, were obtained from a population-based cross-sectional survey conducted in Iran, 2015. The long-term effect of earthquake was assessed using the Mental Health Continuum-Short Form questionnaire. A one-way multivariate analysis of covariance (MANCOVA using SPSS (version 22 was used in data analysis.Results: Older adults scored significantly a higher level of overall positive mental health (mean [M]=34.31, standard deviation [SD]=10.52 than younger age group (M=27.48, SD=10.56, t=-4.41; P<0.001. Results of MANCOVA revealed a statistically significant difference between older and young adults on the combined positive mental health subscales (F(3,317=6.95; P<0.001, after controlling for marital status, sex, and employment status.Conclusion: The present findings showing a higher level of positive mental health among elderly earthquake survivors compared with their younger counterparts in the wake of natural disasters suggest that advancing age per se does not contribute to increasing vulnerability. Keywords

  14. Evidence for involvement of medium chain acyl-CoA dehydrogenase in the metabolism of phenylbutyrate.

    Science.gov (United States)

    Kormanik, Kaitlyn; Kang, Heejung; Cuebas, Dean; Vockley, Jerry; Mohsen, Al-Walid

    2012-12-01

    Sodium phenylbutyrate is used for treating urea cycle disorders, providing an alternative for ammonia excretion. Following conversion to its CoA ester, phenylbutyryl-CoA is postulated to undergo one round of β-oxidation to phenylacetyl-CoA, the active metabolite. Molecular modeling suggests that medium chain acyl-CoA dehydrogenase (MCAD; EC 1.3.99.3), a key enzyme in straight chain fatty acid β-oxidation, could utilize phenylbutyryl-CoA as substrate. Moreover, phenylpropionyl-CoA has been shown to be a substrate for MCAD and its intermediates accumulate in patients with MCAD deficiency. We have examined the involvement of MCAD and other acyl-CoA dehydrogenases (ACADs) in the metabolism of phenylbutyryl-CoA. Anaerobic titration of purified recombinant human MCAD with phenylbutyryl-CoA caused changes in the MCAD spectrum that are similar to those induced by octanoyl-CoA, its bona fide substrate, and unique to the development of the charge transfer ternary complex. The calculated apparent dissociation constant (K(D app)) for these substrates was 2.16 μM and 0.12 μM, respectively. The MCAD reductive and oxidative half reactions were monitored using the electron transfer flavoprotein (ETF) fluorescence reduction assay. The catalytic efficiency and the K(m) for phenylbutyryl-CoA were 0.2 mM 34(-1)·sec(-1) and 5.3 μM compared to 4.0 mM(-1)·sec(-1) and 2.8 μM for octanoyl-CoA. Extracts of wild type and MCAD-deficient lymphoblast cells were tested for the ability to reduce ETF using phenylbutyryl-CoA as substrate. While ETF reduction activity was detected in extracts of wild type cells, it was undetectable in extracts of cells deficient in MCAD. The results are consistent with MCAD playing a key role in phenylbutyrate metabolism. Copyright © 2012 Elsevier Inc. All rights reserved.

  15. Dihydrogen phosphate-water tape and layers vs dihydrogen phosphate layers tuned by hydrophobic isomeric pyridine-diamine functionalized molecules

    Science.gov (United States)

    Huang, Jing; Liu, Tong-Peng; Huo, Li-Hua; Deng, Zhao-Peng; Gao, Shan

    2017-01-01

    Assembly of six isomeric pyridine-diamine-based molecules, N,N‧-bis(pyridin-4-ylmethyl)ethane-1,2-diamine (M1), N,N‧-bis(pyridin-3-ylmethyl)ethane-1,2-diamine (M2), N,N‧-bis(pyridin-2-ylmethyl)ethane-1,2-diamine (M3), N,N‧-bis(pyridin-4-ylmethyl)propane-1,3-diamine (M4), N,N‧-bis(pyridin-3-ylmethyl)propane-1,2-diamine (M5), and N,N‧-bis(pyridin-2-ylmethyl)propane-1,3-diamine (M6), with phosphoric acid (H3PO4) in different ratio (1:2 and 1:4), leads to the formation of nine salts, H2M12+·2H2PO4-·4H2O (1), H2M22+·2H2PO4-·2H2O (2), H2M32+·2H2PO4-·2H2O (3), H4M14+·4H2PO4- (4), H4M24+·4H2PO4- (5), H4M34+·4H2PO4- (6), H2M42+·2H2PO4-·3H2O (7), 2H2M52+·4H2PO4-·2H3PO4 (8), and H2M62+·2H2PO4- (9), which have been characterized by elemental analysis, IR, TG, PL, powder and single-crystal X-ray diffraction. Structural analyses indicate that hydrogen-bonding patterns of H2PO4- anions, conformation of protonated cations can effectively influence the supramolecular architectures through diverse non-covalent interactions. Hydrous salts 1-3 and 7 present 2D and 3D host-guest supramolecular networks, in which the connection of H2PO4- anions and water molecules generates diverse tape and layer motifs. H2PO4- anions in anhydrous salts 4-6 interconnect with each other through hydrogen bonds to form two types of layers, which are joined by discrete H4M4+ cations into 3D inorganic-organic hybrid supramolecular networks. Salts 8-9 also present 2D and 3D host-guest supramolecular networks where the interconnection of H2PO4- anions and its combination with H3PO4 molecules leads to diverse layers. Luminescent analyses indicate that salts 1-9 exhibit violet and blue emission maximum in the range of 390-467 nm at room temperature.

  16. Interaction between Nbp35 and Cfd1 proteins of cytosolic Fe-S cluster assembly reveals a stable complex formation in Entamoeba histolytica.

    Directory of Open Access Journals (Sweden)

    Shadab Anwar

    Full Text Available Iron-Sulfur (Fe-S proteins are involved in many biological functions such as electron transport, photosynthesis, regulation of gene expression and enzymatic activities. Biosynthesis and transfer of Fe-S clusters depend on Fe-S clusters assembly processes such as ISC, SUF, NIF, and CIA systems. Unlike other eukaryotes which possess ISC and CIA systems, amitochondriate Entamoeba histolytica has retained NIF & CIA systems for Fe-S cluster assembly in the cytosol. In the present study, we have elucidated interaction between two proteins of E. histolytica CIA system, Cytosolic Fe-S cluster deficient 1 (Cfd1 protein and Nucleotide binding protein 35 (Nbp35. In-silico analysis showed that structural regions ranging from amino acid residues (P33-K35, G131-V135 and I147-E151 of Nbp35 and (G5-V6, M34-D39 and G46-A52 of Cfd1 are involved in the formation of protein-protein complex. Furthermore, Molecular dynamic (MD simulations study suggested that hydrophobic forces surpass over hydrophilic forces between Nbp35 and Cfd1 and Van-der-Waal interaction plays crucial role in the formation of stable complex. Both proteins were separately cloned, expressed as recombinant fusion proteins in E. coli and purified to homogeneity by affinity column chromatography. Physical interaction between Nbp35 and Cfd1 proteins was confirmed in vitro by co-purification of recombinant Nbp35 with thrombin digested Cfd1 and in vivo by pull down assay and immunoprecipitation. The insilico, in vitro as well as in vivo results prove a stable interaction between these two proteins, supporting the possibility of its involvement in Fe-S cluster transfer to target apo-proteins through CIA machinery in E. histolytica. Our study indicates that initial synthesis of a Fe-S precursor in mitochondria is not necessary for the formation of Cfd1-Nbp35 complex. Thus, Cfd1 and Nbp35 with the help of cytosolic NifS and NifU proteins can participate in the maturation of non-mitosomal Fe-S proteins

  17. Exploring individual differences in online and face-to-face help-seeking intentions in case of impending mental health problems: The role of adult attachment, perceived social support, psychological distress and self-stigma

    Directory of Open Access Journals (Sweden)

    Jennifer Apolinário-Hagen

    2016-11-01

    Full Text Available Background: Even though common mental health problems such as depression are a global burden calling for efficient prevention strategies, still many distressed individuals face hurdles to access public mental healthcare. Thus, computerized Internet-based psychological services have been suggested as viable approach to overcome barriers, such as self-stigma, and to inform the access to professional support on a large scale. However, little research has targeted predictors of online and face-to-face help-seeking intentions. Objective: This study aimed at determining whether associations between attachment insecurity and the willingness to seek online versus face-to-face counselling in case of impending emotional problems are mediated by both perceived social support and psychological distress and moderated by self-stigma. Methods: Data was collected from 301 adults from the German-speaking general population (age: M = 34.42, SD = 11.23; range: 18 - 65 years; 72.1% female through an anonymous online survey. Determinants of seeking help were assessed with the self-report measures Experiences in Close Relationship-Scale, Perceived Stress Questionnaire, ENRICHD-Social Support Inventory and an adapted version of the General Help Seeking Questionnaire (i.e. case vignette. Mediation analyses were performed with the SPSS-macro PROCESS by Hayes. Results: About half of the sample indicated being not aware of online counselling. As expected, insecure attachment was associated with less perceived social support and increased psychological distress. Mediational analyses revealed negative relationships between both attachment avoidance and self-stigma with face-to-face help-seeking intentions. Moreover, the relationship between attachment anxiety and the willingness to seek face-to-face counselling was mediated by social support. In contrast, none of the predictors of online counselling was statistically significant. Conclusions: Overall, this study identified

  18. Chemical profile analysis and comparison of two versions of the classic TCM formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MSn.

    Science.gov (United States)

    Zhang, Ya-Zhou; Xu, Feng; Yi, Tao; Zhang, Jian-Ye; Xu, Jun; Tang, Yi-Na; He, Xi-Chen; Liu, Jing; Chen, Hu-Biao

    2014-04-30

    Danggui Buxue Tang (DBT) is a Traditional Chinese Medicine (TCM) formula primarily used to treat symptoms associated with menopause in women. Usually, DBT is composed of one portion of Radix Angelicae Sinensis (RAS) and five portions of Radix Astragali (RA). Clinically, Radix Hedysari (RH) is sometimes used by TCM physicians to replace RA in DBT. In order to verity whether the chemical constituents of the DBT1 (RA:RAS = 5:1, w/w) and DBT2 (RH:RAS = 5:1, w/w) share similarities the chemical profiles of the two DBTs crude extracts and urine samples were analyzed and compared with the aid of HPLC-DAD-ESI-IT-TOF-MSn, which determines the total ion chromatogram (TIC) and multi-stage mass spectra (MSn). Then, the DBT1 and DBT2 were identified and compared on the basis of the TIC and the MSn. In the first experiment (with crude extracts), 69 compounds (C1-C69) were identified from the DBT1; 46 compounds (c1-c46) were identified from the DBT2. In the second experiment(with urine samples), 44 compounds (M1-M44) were identified from the urine samples of rats that had been administered DBT1, and 34 compounds (m1-m34) were identified from the urine samples of rats that had been administered DBT2. Identification and comparison of the chemical compositions were carried out between the DBT1 and DBT2 of the crude extracts and urine samples respectively. Our results showed that the two crude extracts of the DBTs have quite different chemical profiles. The reasons for their differences were that the special astragalosides in DBT1 and the isoflavonoid glycosides formed the malonic acid esters undergo single esterification and acetyl esters undergo acetylation in DBT1. In contrast, the urine from DBT1-treated rats strongly resembled that of DBT2-treated rats. These metabolites originate mainly from formononetin, calycosin and their related glycosides, and they were formed mainly by the metabolic process of reduction, deglycosylation, demethylation, hydrogenation and sulfation. The

  19. ZZ ENDF/B-V, Evaluated Nuclear Data File Version 5

    International Nuclear Information System (INIS)

    Kinsey, R.; Magurno, B.A.; Young, P.G.

    2006-01-01

    Description: Format: ENDF-5; Number Of Groups: continuous energy; Nuclides: (994) 1-H-1, 1-H-2, 1-H-3, 2-He-3, 2-He-4, 3-Li-6, 3-Li-7, 4-Be-9, 5-B-10, 5-B-11, 6-C-0, 7-N-14, 7-N-15, 8-O-16, 8-O-17, 9-F-19, 11-Na-23, 12-Mg-0, 13-Al-27, 14-Si-0, 15-P-31, 16-S-0, 16-S-32, 17-Cl-0, 19-K-0, 20-Ca-0, 22-Ti-0, 23-V-0, 24-Cr-0, 25-Mn-55, 26-Fe-0, 27-Co-59, 27-Co-72-75, 28-Ni-71-78, 29-Cu-72-82, 30-Zn-72-83, 31-Ga-72, 31-Ga-72m, 31-Ga-73-74, 31-Ga-74m, 31-Ga-75-86, 32-Ge-72-73, 32-Ge-73m, 32-Ge-74-75, 32-Ge-75m, 32-Ge-76-77, 32-Ge-77m, 32-Ge-78-79, 32-Ge-79m, 32-Ge-80-88, 33-As-75-82, 33-As-82m, 33-As-83-84, 33-As-84m, 33-As-85-90, 34-Se-74-77, 34-Se-77m, 34-Se-78-79, 34-Se-79m, 34-Se-80-81, 34-Se-81m, 34-Se-82-83, 34-Se-83m, 34-Se-84-93, 35-Br-79, 35-Br-79m, 35-Br-80, 35-Br-80m, 35-Br-81-82, 35-Br-82m, 35-Br-83-84, 35-Br-84m, 35-Br-85-96, 36-Kr-78-79, 36-Kr-79m, 36-Kr-80-81, 36-Kr-81m, 36-Kr-82-83, 36-Kr-83m, 36-Kr-84-85, 36-Kr-85m, 36-Kr-86-97, 37-Rb-85-86, 37-Rb-86m, 37-Rb-87-90, 37-Rb-90m, 37-Rb-91-101, 38-Sr-84-85, 38-Sr-85m, 38-Sr-86-87, 38-Sr-87m, 38-Sr-88-104, 39-Y-89, 39-Y-89m, 39-Y-90, 39-Y-90m, 39-Y-91, 39-Y-91m, 39-Y-92-93, 39-Y-93m, 39-Y-94-96, 39-Y-96m, 39-Y-97, 39-Y-97m, 39-Y-98, 39-Y-98m, 39-Y-99-107, 40-Zr-90, 40-Zr-90m, 40-Zr-91-110, 41-Nb-93, 41-Nb-93m, 41-Nb-94, 41-Nb-94m, 41-Nb-95, 41-Nb-95m, 41-Nb-96-97, 41-Nb-97m, 41-Nb-98, 41-Nb-98m, 41-Nb-99, 41-Nb-99m, 41-Nb-100, 41-Nb-100m, 41-Nb-101-102, 41-Nb-102m, 41-Nb-103-104, 41-Nb-104m, 41-Nb-105-112, 42-Mo-92-93, 42-Mo-93m, 42-Mo-94-115, 43-Tc-99, 43-Tc-99m, 43-Tc-100-102, 43-Tc-102m, 43-Tc-103-118, 44-Ru-96-109, 44-Ru-109m, 44-Ru-110-120, 45-Rh-103, 45-Rh-103m, 45-Rh-104, 45-Rh-104m, 45-Rh-105, 45-Rh-105m, 45-Rh-106, 45-Rh-106m, 45-Rh-107-108, 45-Rh-108m, 45-Rh-109, 45-Rh-109m, 45-Rh-110, 45-Rh-110m, 45-Rh-111-123, 46-Pd-102-107, 46-Pd-107m, 46-Pd-108-109, 46-Pd-109m, 46-Pd-110-111, 46-Pd-111m, 46-Pd-112-126, 47-Ag-107-108, 47-Ag-108m, 47-Ag-109-110, 47-Ag-110m, 47-Ag-111, 47-Ag-111m, 47-Ag-112-113, 47-Ag

  20. Incorporation of cooling-induced crystallisation into a 2-dimensional axisymmetric conduit heat flow model

    Science.gov (United States)

    Heptinstall, D. A.; Neuberg, J. W.; Bouvet de Maisonneuve, C.; Collinson, A.; Taisne, B.; Morgan, D. J.

    2015-12-01

    Heat flow models can bring new insights into the thermal and rheological evolution of volcanic systems. We shall investigate the thermal processes and timescales in a crystallizing, static magma column, with a heat flow model of Soufriere Hills Volcano (SHV), Montserrat. The latent heat of crystallization is initially computed with MELTS, as a function of pressure and temperature for an andesitic melt (SHV groundmass starting composition). Three fractional crystallization simulations are performed; two with initial pressures of 34MPa (runs 1 & 2) and one of 25MPa (run 3). Decompression rate was varied between 0.1MPa/°C (runs 1 & 3) and 0.2MPa/°C (run 2). Natural and experimental matrix glass compositions are accurately reproduced by all MELTS runs. The cumulative latent heat released for runs 1, 2 and 3 differs by less than 9% (8.69e5 J/kg*K, 9.32e5 J/kg*K, and 9.49e5 J/kg*K respectively). The 2D axisymmetric conductive cooling simulations consider a 30m-diameter conduit that extends from the surface to a depth of 1500m (34MPa). The temporal evolution of temperature is closely tracked at depths of 10m, 750m and 1400m in the center of the conduit, at the conduit walls, and 20m from the walls into the host rock. Following initial cooling by 7-15oC at 10m depth inside the conduit, the magma temperature rebounds through latent heat release by 32-35oC over 85-123 days to a maximum temperature of 1002-1005oC. At 10 m depth, it takes 4.1-9.2 years for the magma column to cool over 108-130oC and crystallize to 75wt%, at which point it cannot be easily remobilized. It takes 11-31.5 years to reach the same crystallinity at 750-1400m depth. We find a wide range in cooling timescales, particularly at depths of 750m or greater, attributed to the initial run pressure and dominant latent heat producing crystallizing phases (Quartz), where run 1 cools fastest and run 3 cools slowest. Surface cooling by comparison has the strongest influence on the upper tens of meters in all

  1. Incorporation of cooling-induced crystallization into a 2-dimensional axisymmetric conduit heat flow model

    Science.gov (United States)

    Heptinstall, David; Bouvet de Maisonneuve, Caroline; Neuberg, Jurgen; Taisne, Benoit; Collinson, Amy

    2016-04-01

    Heat flow models can bring new insights into the thermal and rheological evolution of volcanic 3 systems. We shall investigate the thermal processes and timescales in a crystallizing, static 4 magma column, with a heat flow model of Soufriere Hills Volcano (SHV), Montserrat. The latent heat of crystallization is initially computed with MELTS, as a function of pressure and temperature for an andesitic melt (SHV groundmass starting composition). Three fractional crystallization simulations are performed; two with initial pressures of 34MPa (runs 1 & 2) and one of 25MPa (run 3). Decompression rate was varied between 0.1MPa/° C (runs 1 & 3) and 0.2MPa/° C (run 2). Natural and experimental matrix glass compositions are accurately reproduced by all MELTS runs. The cumulative latent heat released for runs 1, 2 and 3 differs by less than 9% (8.69E5 J/kg*K, 9.32E5 J/kg*K, and 9.49E5 J/kg*K respectively). The 2D axisymmetric conductive cooling simulations consider a 30m-diameter conduit that extends from the surface to a depth of 1500m (34MPa). The temporal evolution of temperature is closely tracked at depths of 10m, 750m and 1400m in the centre of the conduit, at the conduit walls, and 20m from the walls into the host rock. Following initial cooling by 7-15oC at 10m depth inside the conduit, the magma temperature rebounds through latent heat release by 32-35oC over 85-123 days to a maximum temperature of 1002-1005oC. At 10m depth, it takes 4.1-9.2 years for the magma column to cool by 108-131oC and crystallize to 75wt%, at which point it cannot be easily remobilized. It takes 11-31.5 years to reach the same crystallinity at 750-1400m depth. We find a wide range in cooling timescales, particularly at depths of 750m or greater, attributed to the initial run pressure and the dominant latent heat producing crystallizing phase, Albite-rich Plagioclase Feldspar. Run 1 is shown to cool fastest and run 3 cool the slowest, with surface emissivity having the strongest cooling

  2. Construção e validação da Escala de Imprevisibilidade Familiar na Infância (EIFI Construction and validation of the Family Unpredictability Scale in Childhood

    Directory of Open Access Journals (Sweden)

    Anna Beatriz Carnielli Howat-Rodrigues

    2012-01-01

    Full Text Available Este estudo teve o objetivo de construir e validar uma medida psicológica denominada de Escala de Imprevisibilidade Familiar na Infância (EIFI para a população brasileira. Participaram 394 pessoas, sendo 158 adultos em conflito com a lei que já haviam passado pela prisão (média de idade=34,23 anos; DP=10,17, 122 estudantes universitários (M=19,26; DP=2,06 e 114 mulheres com idade superior a 40 anos e escolaridade a partir de ensino médio (M=51,19; DP=8,64. Foram conduzidos procedimentos de validade de conteúdo, face e construto. A análise fatorial gerou como produto um instrumento com estrutura de quatro dimensões (cuidado/apoio, recursos financeiros, alimentação e disciplina com índices de confiabilidade satisfatórios para todas elas. Concluímos que a EIFI foi validada para a população brasileira apresentando-se no cenário nacional como um instrumento para medida de imprevisibilidade familiar na infância.This study aimed to construct and validate a psychological measure called Family Unpredictability Scale in Childhood for the Brazilian population. The study included 394 people: 158 adults in conflict with the law that had already been in prison (M=34.23 years; SD=10.17, 122 college students (M=19.26; SD=2.06, and 114 women above 40 years old and, at least, high school schooling (M=51.19; SD=8.64. Procedures of content, face and construct validity were conducted. The factor analysis generated an instrument structured in four dimensions (care/support, financial resources, food and discipline with satisfactory reliability indexes for all of them. It was concluded that Family Unpredictability Scale in Childhood was validated to Brazilian population and that it can be used as an instrument to measure family unpredictability in childhood on the national scene.

  3. Y-chromosome phylogeographic analysis of the Greek-Cypriot population reveals elements consistent with Neolithic and Bronze Age settlements.

    Science.gov (United States)

    Voskarides, Konstantinos; Mazières, Stéphane; Hadjipanagi, Despina; Di Cristofaro, Julie; Ignatiou, Anastasia; Stefanou, Charalambos; King, Roy J; Underhill, Peter A; Chiaroni, Jacques; Deltas, Constantinos

    2016-01-01

    The archeological record indicates that the permanent settlement of Cyprus began with pioneering agriculturalists circa 11,000 years before present, (ca. 11,000 y BP). Subsequent colonization events followed, some recognized regionally. Here, we assess the Y-chromosome structure of Cyprus in context to regional populations and correlate it to phases of prehistoric colonization. Analysis of haplotypes from 574 samples showed that island-wide substructure was barely significant in a spatial analysis of molecular variance (SAMOVA). However, analyses of molecular variance (AMOVA) of haplogroups using 92 binary markers genotyped in 629 Cypriots revealed that the proportion of variance among the districts was irregularly distributed. Principal component analysis (PCA) revealed potential genetic associations of Greek-Cypriots with neighbor populations. Contrasting haplogroups in the PCA were used as surrogates of parental populations. Admixture analyses suggested that the majority of G2a-P15 and R1b-M269 components were contributed by Anatolia and Levant sources, respectively, while Greece Balkans supplied the majority of E-V13 and J2a-M67. Haplotype-based expansion times were at historical levels suggestive of recent demography. Analyses of Cypriot haplogroup data are consistent with two stages of prehistoric settlement. E-V13 and E-M34 are widespread, and PCA suggests sourcing them to the Balkans and Levant/Anatolia, respectively. The persistent pre-Greek component is represented by elements of G2-U5(xL30) haplogroups: U5*, PF3147, and L293. J2b-M205 may contribute also to the pre-Greek strata. The majority of R1b-Z2105 lineages occur in both the westernmost and easternmost districts. Distinctively, sub-haplogroup R1b- M589 occurs only in the east. The absence of R1b- M589 lineages in Crete and the Balkans and the presence in Asia Minor are compatible with Late Bronze Age influences from Anatolia rather than from Mycenaean Greeks.

  4. Affective Commitment to Organizations: A Comparison Study of Reverse Mentoring Versus Traditional Mentoring Among Millennials

    Directory of Open Access Journals (Sweden)

    Catrin Hechl

    2017-08-01

    Full Text Available A current topic of interest in management and organization research is the phenomenon of a generation shift in the workforce and how this shift will affect organizations in the near future.  Millennials represent the largest generational cohort in the American workforce.  Organizations find themselves challenged with retention efforts as Millennials tend to leave an organization after short tenures.  The problem this study addressed is the high turnover rates among millennial employees. Specifically, it was unknown whether Millennials who received reverse mentoring evidenced greater affective commitment to the organization as compared to Millennials who received standard mentoring.  The purpose of this study was to investigate the hypothesis that Millennials who received reverse mentoring evidenced greater affective commitment to the organization as compared to Millennials who received standard mentoring.  A two group post-test only quasi-experimental design was conducted.  A total of 90 participants (45 per group completed the survey.  The survey was conducted by Qualtrics, an online survey company.  The sample population included male and female individuals, born between 1982 and 1998, employed by all types of organizations in the United States and participating in a mentoring program at the time the survey was taken.  Affective commitment was greater in the reverse mentoring group (M = 36.683, SE = .959 compared to the traditional mentoring group (M = 34.984, SE = .959.  However, after adjustment for quality of relationship (LMX and length and frequency of mentoring (LFM there was no statistically significant difference (p < .05 between traditional mentoring and reverse mentoring on affective commitment to the organization indicated by F(1,86 = 1.569, p = .214.  Additional results of this study showed that two-thirds of the surveyed millennial employees had already exceeded the average length of employment of 12 to 18 months with

  5. Comparative Evaluation of Marginal Adaptation and Fracture Strength of Different Ceramic Inlays Produced by CEREC Omnicam and Heat-Pressed Technique.

    Science.gov (United States)

    Oz, F D; Bolay, S

    2018-01-01

    The aim of this in vitro study was to evaluate marginal adaptation and fracture strength of inlays produced by CEREC Omnicam using different types of blocs and heat-pressed technique. Methods: Seventy-five extracted human mandibular molars were divided randomly into 5 groups ( n =15). 60 molars in four groups received MOD inlay preparations. Experimental groups were CO: Intact teeth, EC: IPS e.max CAD and CEREC, LU: Lava Ultimate and CEREC, EL: IPS Empress CAD and CEREC, EP: IPS Empress Esthetic ingots and heat-pressed technique. Marginal gap measurements were taken with a stereomicroscope. Restorations were cemented with Variolink N and stored in distilled water at 37°C for 24 hours. All samples were subjected to thermocycling. The fracture strength of specimens was determined at a 0.5 mm/min crosshead speed until fracture. Fracture modes were determined. Statistical analyses were performed using one-way analysis of variance for fracture strength data and Kruskal-Wallis for marginal gap data ( p =0.05). The mean marginal gap size of EC, LU, EL, and EP were 33.54  µ m, 33.77  µ m, 34.23  µ m, and 85.34  µ m, respectively. EP had statistically higher values than other groups. The fracture strength values were significantly higher in the intact teeth group (3959,00 ± 1279,79 N) than those of restored groups EC (2408,00 ± 607,97 N), LU (2206,73 ± 675,16), EL (2573.27 ± 644,73) ve EP (2879,53 ± 897,30). Inlays fabricated using CEREC Omnicam demonstrated better marginal adaptation than inlays produced with heat-pressed technique, whereas fracture strength values of inlays fabricated with different type of blocks using CEREC Omnicam exhibited similarity to those fabricated with heat-pressed technique.

  6. Caracterización morfológica, biológica y genética de un aislamiento Colombiano de granulovirus de Erinnyis ello (L. (Lepidoptera: Sphingidae

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    Gloria Patricia Barrera Cubillos

    2014-07-01

    Full Text Available Título en español: Caracterización morfológica, biológica y genética de un aislamiento Colombiano de granulovirus de Erinnyis ello (L. (Lepidoptera: Sphingidae Título en ingles: Characterization of a Colombian isolate of Erinnyis ello granulovirus (L. (Lepidoptera: Sphingidae Titulo corto: Granulovirus de E. ello Resumen: El gusano cachón Erinnyis ello (L. es una plaga polífaga que puede causar graves pérdidas en cultivos de caucho. El uso de granulovirus representa una alternativa interesante para el control biológico de este insecto. Tres aislamientos colombianos del granulovirus de E. ello (EeGV recuperados en larvas de campo, fueron caracterizados morfológica y molecularmente. Los cuerpos de inclusión de los tres aislamientos presentaron forma ovoide con una única nucleocápside, con tamaño promedio de 302,9 ± 22 x 181,5 ± 16 nm. El análisis de los perfiles de restricción con diferentes endonucleasas no mostró diferencias entre los tres aislamientos, lo cual sugiere que son muestras de la misma cepa viral, denominada VG010, cuyo tamaño del genoma se estimó en 88,7 Kb. El análisis de las relaciones filogenéticas basado en las secuencias de lef-8, lef-9 y gran mostró con alta consistencia la estrecha relación entre VG010 y un aislamiento de EeGV (M34-4 previamente descrito, lo cual sugiere que son variantes genotípicas de la misma especie viral. La eficacia del aislamiento VG010 en condiciones de laboratorio sobre larvas de segundo y cuarto estadio fue de 100 % y 64 % respectivamente, mientras que la concentración letal media (CL50 fue 4,3 x 103 CI/mL. La productividad viral, osciló entre 2,1 x 109 y 3,8 x 109 CI/gramo de larva. Estos resultados representan la base para el desarrollo de un nuevo bioinsecticida para el control de la plaga en campo. Palabras clave: Gusano cachón. Baculovirus. Control biológico. Filogenia.  Abstract: Erinnyis ello (L. is a polyphagous lepidopteran pest that may cause serious

  7. Evaluation of Metal Toxicity in Streams Affected by Abandoned Mine Lands, Upper Animas River Watershed, Colorado

    Science.gov (United States)

    Besser, John M.; Allert, Ann L.; Hardesty, Douglas K.; Ingersoll, Christopher G.; May, Thomas W.; Wang, Ning; Leib, Kenneth J.

    2001-01-01

    water; (3) to develop site-specific thresholds for toxicity of Zn and Cu in stream water; and (4) to develop models of the contributions of Cu and Zn to toxicity of stream water, which may be used to characterize toxicity before and after planned remediation efforts. We evaluated the toxicity of metal-contaminated sediments by conducting sediment toxicity tests with two species of benthic invertebrates, the midge, Chironomus tentans. and the amphipod, Hyalella azteca. Laboratory toxicity tests with both taxa, exposed to fine stream-bed sediments collected in September 1997, showed some evidence of sediment toxicity, as survival of midge larvae in sediments from Cement Creek (C48) and lower Mineral Creek (M34), and growth of amphipods in sediments from these sites and three Animas River sites (A68, Animas at Silverton; A72, Animas below Silverton, and A73, Animas at Elk Park) were significantly reduced compared to a reference site, South Mineral Creek (SMC) . Amphipods were also exposed to site water and fine stream-bed sediment, separately and in combination, during the late summer low flow period (August-September) of 1998. In these studies, stream water, with no sediment present, from all five sites tested (same sites as above, except C48) caused 90% to 100% mortality of amphipods. In contrast, significant reductions in survival of amphipods occurred at two sites (A72 and SMC) in exposures with field-collected sediment plus stream water, and at only one site (A72) in exposures with sediments and clean overlying water. Concentrations of Zn, Pb, Cu, and Cd were high in both sediment and pore water (interstitial water) from most sites tested, but greatest sediment toxicity was apparently associated with greater concentrations of Fe and/or Al in sediments. These results suggest that fine stream-bed sediments of the more contaminated stream reaches of the upper Animas River watershed are toxic to benthic invertebrates, but that these impacts are less serious than tox

  8. Positive and Negative Thinking in Tinnitus: Factor Structure of the Tinnitus Cognitions Questionnaire.

    Science.gov (United States)

    Handscomb, Lucy E; Hall, Deborah A; Shorter, Gillian W; Hoare, Derek J

    Researchers and clinicians consider thinking to be important in the development and maintenance of tinnitus distress, and altering thoughts or thinking style is an object of many forms of psychological therapy for tinnitus. Those working with people with tinnitus require a reliable, psychometrically robust means of measuring both positive and negative thinking related to it. The Tinnitus Cognitions Questionnaire (TCQ) was designed as such a measure and its authors showed it to be reliable, with good psychometric properties. However, no research teams have yet carried out independent validation. This study aimed to use the TCQ to investigate thinking amongst members of the general population with both bothersome and nonbothersome tinnitus and also to verify its factor structure. Three hundred forty-two members of the public with tinnitus completed the TCQ online or on paper. They also rated their tinnitus on a scale as "not a problem," "a small problem," "a moderate problem," "a big problem," or a "very big problem." The authors tested the original factor structure of the TCQ using confirmatory factor analysis and then calculated the mean scores for each item, comparing mean total scores across "problem categories" for the full questionnaire and for the positive and negative subscales. The original two-factor structure of the TCQ was a good fit to the data when the correlation between positive and negative factors was fixed at zero (root mean square error of approximation = 0.064, 90% confidence interval = 0.058 to 0.070). Items pertaining to wishing the tinnitus would go away and despairing that it would ever get better had the highest mean scores. The mean total score for the "no problem" group (M = 31.17, SD = 16.03) was not significantly different from the mean total score for the "small problem" group (M = 34.00, SD = 12.44, p = 0.99). Differences between mean scores for all other groups were statistically significant. For the negative subscale, differences

  9. Modifications to an existing waste containment structure at Niagara Falls Storage Site

    International Nuclear Information System (INIS)

    Paez-Restrepo, A.; Darby, J.W.

    1992-01-01

    The Niagara Falls Storage Site (NFSS), located near Lewiston, New York, is an interim waste containment facility for low-level radioactive waste. The facility was completed in 1986 and is managed for the Department of Energy (DOE) by Bechtel National, Inc. (BNI) as part of the Formerly Utilized Sites Remedial Action Program (FUSRAP). The waste containment structure (WCS) at NFSS is approximately 297 m (975 ft) long and 137 m (450 ft) wide and reaches a maximum height of 10.4 m (34 ft). The peripheral slopes rise at an angle of 3:1 (h:v) for a width of about 16.8 m (55 ft), where the inclination decreases to 7.5%. The apex of the pile is higher at the south end, sloping about 1.2 m (4 ft) to the north. The interim layered cap consists of 0.9 m (3 ft) of clay overlain by 0.45 m (1.5 ft) of topsoil. The uppermost 15 cm (6 in.) of soil was loosely compacted to permit the development of a grass cover. In the summer of 1991, approximately 2,677 m 3 (3,500 yd 3 ) of additional contaminated soil and material in temporary storage elsewhere at NFSS was incorporated into the WCS. To accommodate the waste, a portion of the cap roughly centered with the pile [including 0.45 m (1.5 ft) of topsoil and 0.6 m (2 ft) of clay cap] was removed from an area 99 m (325 ft) long and 58.5 m (192 ft) wide, leaving a minimum of 0.3 m (I ft) of clay over the old waste as a radiation and radon barrier. The newly incorporated waste forms a layer 0.6 m (2 ft) thick, replacing the clay portion of the excavated cap. The waste is contained laterally by the old cap and sealed by a new cap, which also consists of 0.9 m (3 ft) of compacted clay and 0.45 m (1.5 ft) of topsoil. A transition zone about 6.1 m (20 ft) wide feathers the new cap to the old cap (see Fig. 3). Except for the uppermost 10.5 to 15.2 cm (4 to 6 in.) of vegetated topsoil, the excavated cap materials were stockpiled and reused in constructing the new cap. Additional material required to complete cap construction was imported from

  10. Conhecimento dos pais sobre alimentação: construção e validação de um questionário de alimentação infantil Conocimiento de los padres sobre la alimentación: construcción y validación de un cuestionario sobre alimentación infantil Parents’ knowledge about feeding: construction and validation of a child feeding questionnaire

    Directory of Open Access Journals (Sweden)

    Maria da Graça ferreira Aparício Costa

    2012-03-01

    Full Text Available Enquadramento: instrumentos válidos que permitam obter informação sobre os conhecimentos e as práticas que medeiam a alimentação das crianças revelam-se de grande valor para o planeamento de ações de promoção da saúde mais dirigidas. Objetivo: descrever o processo de construção e validação dum questionário de avaliação de Conhecimentos dos Pais sobre Alimentação Infantil. Metodologia: participaram 559 pais de crianças pré-escolares, residentes no centro de Portugal, com média de idades, mãe (M= 34,43; Dp= 5,0; pai (M= 36,47; Dp=5,45 anos. Foi analisada a consistência interna e a validade de constructo. Resultados: o Alfa de Cronbach final, (Alfa=0,942, revelou boa consistência interna, com correlação positiva e significativa na nota global com e sem o item incluído. A análise fatorial de componentes principais, seguida de rotação varimax e gráfico scree plots, extraiu quatro fatores explicando 39,59% da variância total. A composição dos fatores sugere os significados: Fator 1 - Alimentação e Saúde; Fator 2 - Regras Alimentares; Fator 3 - Crenças Alimentares Familiares; Fator 4 - Variedade/Diversidade no Padrão Alimentar. Conclusão: as propriedades psicométricas do QAI certificam a sua qualidade, enquanto ferramenta a utilizar nas atividades de promoção da saúde, dirigida à identificação das necessidades de (informação dos pais sobre alimentação infantil.Antecedentes: instrumentos capaces de conseguir información acerca de los conocimientos y prácticas que intervienen en la alimentación de los niños, llegan a ser valioso para la planificación de acciones más centradas en la promoción de la salud. Objetivo: describir el proceso de construcción y validación de un cuestionario de evaluación del conocimiento de los padres sobre la alimentación infantil. Métodos: participaron 559 padres de niños en edad preescolar que viven en el centro de Portugal cuyas madres tienen una edad media de

  11. CORRELATION OF WELL-BEING WITH RESILIENCE AND AGE

    Directory of Open Access Journals (Sweden)

    Guna Svence

    2015-06-01

    correlation between the age and well-being? A data analysis is made in two parts within the framework of this research. Part 1 is dedicated to the examination of the research questions: 1 what kind of correlation does exist between the indicators of well-being and resilience? and 2 which well-being indicators predict these resilience indicators?, people of the Latvian population aged from 16 to 65 years were selected, 28.3% of men (N=184, M=32.1, SD=11,0 years, which was made using the secondary data of two studies (Madžule, 2013; Tauriņa, 2013. Part 2 of the research deals with the examination of the research questions 3 is there any correlation between the age and well-being?, people aged from 16 to 65 years were chosen, 24.5% of men (N=351, M=34.4, SD=11.5, which was made using the secondary data of three studies (Majors & Majore, 2009; Madžule, 2013; Tauriņa, 2013. It was found out, that the indicators of well-being have a direct and a rather close correlation with the indicators of resilience which provided the answer to the research question, what kind of correlation between the indicators of well-being and resilience exists. The indicators of the well-being dimension indicators have a little or rather close correlation with the parameters of the resilience dimension(0.29