TMBP200, a XMAP215 homologue of tobacco BY-2 cells, has an essential role in plant mitosis.

Science.gov (United States)

Yasuhara, Hiroki; Oe, Yuki

2011-07-01

TMBP200 from tobacco BY-2 cells is a member of the highly conserved family of microtubule-associated proteins that includes Xenopus XMAP215, human TOGp, and Arabidopsis MOR1/GEM1. XMAP215 homologues have an essential role in spindle assembly and function in animals and yeast, but their role in plant mitosis is not fully clarified. Here, we show by immunoblot analysis that TMBP200 levels in synchronously cultured BY-2 cells increased when the cells entered mitosis, thus indicating that TMBP200 plays an important role in mitosis in tobacco. To investigate the role of TMBP200 in mitosis, we employed inducible RNA interference to silence TMBP200 expression in BY-2 cells. The resulting depletion of TMBP200 caused severe defects in bipolar spindle formation and resulted in the appearance of multinucleated cells with variable-sized nuclei. This finding indicates that TMBP200 has an essential role in bipolar spindle formation and function.

Simultaneous analysis of cerebrospinal fluid biomarkers using microsphere-based xMAP multiplex technology for early detection of Alzheimer's disease.

Science.gov (United States)

Kang, Ju-Hee; Vanderstichele, Hugo; Trojanowski, John Q; Shaw, Leslie M

2012-04-01

The xMAP-Luminex multiplex platform for measurement of Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarkers using Innogenetics AlzBio3 immunoassay reagents that are for research use only has been shown to be an effective tool for early detection of an AD-like biomarker signature based on concentrations of CSF Aβ(1-42), t-tau and p-tau(181). Among the several advantages of the xMAP-Luminex platform for AD CSF biomarkers are: a wide dynamic range of ready-to-use calibrators, time savings for the simultaneous analyses of three biomarkers in one analytical run, reduction of human error, potential of reduced cost of reagents, and a modest reduction of sample volume as compared to conventional enzyme-linked immunosorbant assay (ELISA) methodology. Recent clinical studies support the use of CSF Aβ(1-42), t-tau and p-tau(181) measurement using the xMAP-Luminex platform for the early detection of AD pathology in cognitively normal individuals, and for prediction of progression to AD dementia in subjects with mild cognitive impairment (MCI). Studies that have shown the prediction of risk for progression to AD dementia by MCI patients provide the basis for the use of CSF Aβ(1-42), t-tau and p-tau(181) testing to assign risk for progression in patients enrolled in therapeutic trials. Furthermore emerging study data suggest that these pathologic changes occur in cognitively normal subjects 20 or more years before the onset of clinically detectable memory changes thus providing an objective measurement for use in the assessment of treatment effects in primary treatment trials. However, numerous previous ELISA and Luminex-based multiplex studies reported a wide range of absolute values of CSF Aβ(1-42), t-tau and p-tau(181) indicative of substantial inter-laboratory variability as well as varying degrees of intra-laboratory imprecision. In order to address these issues a recent inter-laboratory investigation that included a common set of CSF pool aliquots from

Evaluation of LABType® SSO HLA Typing using the Luminex Platform: Cord Blood Registry Typing for the Korean Population.

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Roh, Eun-Youn; Song, Eun-Young; Chang, Jee-Young; Yoon, Jong-Hyun; Shin, Sue

2016-08-01

The performance of a new intermediate-resolution method using a PCR-Luminex platform and LABType® SSO A, B DRB1 kits as an HLA typing method for the cord blood (CB) registry of the Korean population was investigated. A total of 1,413 cord blood units (CBUs) were enrolled - 1,382 from Koreans and 31 from non-Koreans or mixed-ancestry individuals. HLA-A, -B, and -DRB1 typing was performed using the LABType® SSO typing kits. HLA typing with the DNA method and 2-digit results are mandatory for the public CB bank in Korea according to the "CB Act." The proportions of ambiguous results in the 2-digit assignment were 14.6% (206/1,413) and 14.8% (205/ 1,382) among the total subjects and the Korean donors, respectively. In the 2-digit resolution, 3 different HLA-A types (69 CBUs), 31 HLA-B types (124 CBUs), and 3 HLA-DRB1 types (13 CBUs) showed ambiguous results. The 'most probable type' to the ambiguous results based on the reported Korean HLA allele frequencies were able to be assigned. The most probable results were 100% consistent with the confirmed types as determined by the HD kits (DRB1) and additional PCR-SBT or PCR-SSP tests (A and B). Luminex technology is more automated and less labor intensive than the conventional SSO typing method, and the results are less affected by differences between inspectors. Although it is not satisfactory as a sole confirmation test and cannot be used as a replacement for the PCR-SBT test, the combination of Luminex technology with LABType® SSO kits and population frequency data provides a proper typing platform that can be used as a qualifying test for CB registries.

Effect of implementing anti-HLA antibody detection by Luminex in the kidney transplant program in Chile.

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Elgueta, S; Fuentes, C; López, M; Hernández, J; Arenas, A; Jiménez, M; Gajardo, J G; Rodríguez, H; Labraña, C

2011-11-01

The development of new highly sensitive, specific technologies to detect HLA antibodies has allowed a better definition of the profile of non-permitted antigens for patients awaiting kidney transplantation. The use of calculated or virtual panel reactive antibodies (CPRA or vPRA) seeks to improve the prediction of positive crossmatches (XM), but increases the proportion of sensitized patients on the waiting list. In 2008-2009, we implemented detection of antibodies using Luminex technology and applied vPRA since 2009. The objective of this study was to evaluate the impact of these innovations in defecting patient sensitization on kidney transplant waiting lists for deceased donors and among transplanted patients. We analyzed the waiting list for 2007 through 2009 and the first semester of 2010, including the patients transplanted in these periods and the XM with deceased donors. We observed an increase in the mean peak PRA of transplanted patients from 7.2% in 2007 to 17.1% in 2010 (P = .001), and in the proportion of patients transplanted with a peak PRA > 50% from 2.8% in 2007 to 15.7% in 2010 (P = .0001), with no increase in the proportion of this population on the waiting lists. There was a concurrent decrease in positive XM among patients with a peak PRA > 50%. The use of vPRA and Luminex permitted a greater number of transplants of patients with peak PRA > 50% and was a good predictor of a positive XM. Published by Elsevier Inc.

Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus.

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Yu, Cui; Yang, Cuiyun; Song, Shaoyi; Yu, Zixiang; Zhou, Xueping; Wu, Jianxiang

2018-04-04

Iris yellow spot virus (IYSV) is an Orthotospovirus that infects most Allium species. Very few approaches for specific detection of IYSV from infected plants are available to date. We report the development of a high-sensitive Luminex xMAP-based microsphere immunoassay (MIA) for specific detection of IYSV. The nucleocapsid (N) gene of IYSV was cloned and expressed in Escherichia coli to produce the His-tagged recombinant N protein. A panel of monoclonal antibodies (MAbs) against IYSV was generated by immunizing the mice with recombinant N protein. Five specific MAbs (16D9, 11C6, 7F4, 12C10, and 14H12) were identified and used for developing the Luminex xMAP-based MIA systems along with a polyclonal antibody against IYSV. Comparative analyses of their sensitivity and specificity in detecting IYSV from infected tobacco leaves identified 7F4 as the best-performed MAb in MIA. We then optimized the working conditions of Luminex xMAP-based MIA in specific detection of IYSV from infected tobacco leaves by using appropriate blocking buffer and proper concentration of biotin-labeled antibodies as well as the suitable ratio between the antibodies and the streptavidin R-phycoerythrin (SA-RPE). Under the optimized conditions the Luminex xMAP-based MIA was able to specifically detect IYSV with much higher sensitivity than conventional enzyme-linked immunosorbent assay (ELISA). Importantly, the Luminex xMAP-based MIA is time-saving and the whole procedure could be completed within 2.5 h. We generated five specific MAbs against IYSV and developed the Luminex xMAP-based MIA method for specific detection of IYSV in plants. This assay provides a sensitive, high-specific, easy to perform and likely cost-effective approach for IYSV detection from infected plants, implicating potential broad usefulness of MIA in plant virus diagnosis.

The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.

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Flor-Parra, Ignacio; Iglesias-Romero, Ana Belén; Chang, Fred

2018-06-04

The organization and number of microtubules (MTs) in a cell depend on the proper regulation of MT nucleation. Currently, the mechanism of nucleation is the most poorly understood aspect of MT dynamics. XMAP215/chTOG/Alp14/Stu2 proteins are MT polymerases that stimulate MT polymerization at MT plus ends by binding and releasing tubulin dimers. Although these proteins also localize to MT organizing centers and have nucleating activity in vitro, it is not yet clear whether these proteins participate in MT nucleation in vivo. Here, we demonstrate that in the fission yeast Schizosaccharomyces pombe, the XMAP215 ortholog Alp14 is critical for efficient MT nucleation in vivo. In multiple assays, loss of Alp14 function led to reduced nucleation rate and numbers of interphase MT bundles. Conversely, activation of Alp14 led to increased nucleation frequency. Alp14 associated with Mto1 and γ-tubulin complex components, and artificially targeting Alp14 to the γ-tubulin ring complexes (γ-TuRCs) stimulated nucleation. In imaging individual nucleation events, we found that Alp14 transiently associated with a γ-tubulin particle shortly before the appearance of a new MT. The transforming acidic coiled-coil (TACC) ortholog Alp7 mediated the localization of Alp14 at nucleation sites but not plus ends, and was required for efficient nucleation but not for MT polymerization. Our findings provide the strongest evidence to date that Alp14 serves as a critical MT nucleation factor in vivo. We suggest a model in which Alp14 associates with the γ-tubulin complex in an Alp7-dependent manner to facilitate the assembly or stabilization of the nascent MT. Copyright © 2018 Elsevier Ltd. All rights reserved.

Multiplex detection of plant pathogens through the luminex magplex bead system

NARCIS (Netherlands)

Vlugt, van der R.A.A.; Raaij, van H.M.G.; Weerdt, de M.; Bergervoet, J.H.W.

2015-01-01

Here we describe a versatile multiplex method for both the serological and molecular detection of plant pathogens. The Luminex MagPlex bead system uses small paramagnetic microspheres (“beads”), either coated with specific antibodies or oligonucleotides, which capture respectively viruses and/or

A Belgian Serosurveillance/Seroprevalence Study of Diphtheria, Tetanus and Pertussis Using a Luminex xMAP Technology-Based Pentaplex

Directory of Open Access Journals (Sweden)

Raissa Nadège Caboré

2016-05-01

Full Text Available Serosurveillance and seroprevalence studies are an essential tool to monitor vaccine-preventable diseases. We have developed a magnetic bead-based pentaplex immunoassay (MIA for the simultaneous detection of IgG antibodies against diphtheria toxin (DT, tetanus toxin (TT, pertussis toxin (PT, filamentous hemagglutinin (FHA and pertactin (Prn. The in-house pentaplex MIA showed a good correlation with commercial ELISAs with correlation coefficients between 0.89 for PT and 0.98 for TT. Intra- and inter-assay variability was <10%. A total of 670 anonymized serum samples collected in 2012 in Belgian adults (ages 20–29.9 years were analyzed. Geometric mean concentrations (GMC were 0.2 (0.13–0.29 IU/mL for DT, 0.63 (0.45–0.82 IU/mL for TT, 3.9 (2.6–5.8 IU/mL for PT, 16.3 (11.7–22.7 IU/mL for FHA and 15.4 (10.1–23.6 IU/mL for Prn. Antibody concentrations were below the protective level of 0.1 IU/mL in 26.4% of the sera for DT and in 8.6% of the sera for TT. Anti-PT IgG concentrations indicative of recent pertussis infection (>125 IU/mL were detected in 1.2% of the subjects. High anti-PT antibodies were not correlated with high antibodies against any of the four other vaccine antigens. This pentaplex MIA will be used for a new large-scale Belgian serosurveillance/seroprevalence study of diphtheria, tetanus and pertussis.

Cytokine profiling of tumor interstitial fluid of the breast and its relationship with lymphocyte infiltration and clinicopathological characteristics

DEFF Research Database (Denmark)

Espinoza, Jaime A.; Jabeen, Shakila; Batra, Richa

2016-01-01

uncharacterized. Moreover, the data obtained regarding the origin of cytokine secretions, the levels of secretion associated with tumor development, and the possible clinical relevance of cytokines remain controversial. Therefore, we profiled 27 cytokines in 78 breast tumor interstitial fluid (TIF) samples, 43...... normal interstitial fluid (NIF) samples, and 25 matched serum samples obtained from BC patients with Luminex xMAP multiplex technology. Eleven cytokines exhibited significantly higher levels in the TIF samples compared with the NIF samples: interleukin (IL)-7, IL-10, fibroblast growth factor-2, IL-13......, IL-7, IL-10, and PDGFβ also exhibited a correlation between the TIF samples and matched sera. In a survival analysis, high levels of IL-5, a hallmark TH2 cytokine, in the TIF samples were associated with a worse prognosis. These findings have important implications for BC immunotherapy research....

The growth speed of microtubules with XMAP215-coated beads coupled to their ends is increased by tensile force

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Trushko, Anastasiya; Schäffer, Erik; Howard, Jonathon

2013-01-01

The generation of pulling and pushing forces is one of the important functions of microtubules, which are dynamic and polarized structures. The ends of dynamic microtubules are able to form relatively stable links to cellular structures, so that when a microtubule grows it can exert a pushing force and when it shrinks it can exert a pulling force. Microtubule growth and shrinkage are tightly regulated by microtubule-associated proteins (MAPs) that bind to microtubule ends. Given their localization, MAPs may be exposed to compressive and tensile forces. The effect of such forces on MAP function, however, is poorly understood. Here we show that beads coated with the microtubule polymerizing protein XMAP215, the Xenopus homolog of Dis1 and chTOG, are able to link stably to the plus ends of microtubules, even when the ends are growing or shrinking; at growing ends, the beads increase the polymerization rate. Using optical tweezers, we found that tensile force further increased the microtubule polymerization rate. These results show that physical forces can regulate the activity of MAPs. Furthermore, our results show that XMAP215 can be used as a handle to sense and mechanically manipulate the dynamics of the microtubule tip. PMID:23964126

Development of an alpha-fetoprotein and Golgi protein 73 multiplex detection assay using xMAP technology.

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Wu, Yun; Ma, Jie; Wang, Yipeng; Zhang, Yonghong; Hou, Yongjin; Zhang, Chunming; Sun, Huanqin; Sun, Jianping; Wang, Zikang; Li, Ning

2018-04-06

Development of a new method to simultaneously detect Alpha-fetoprotein (AFP) and Golgi protein 73 (GP73) from peripheral blood. Anti human AFP and GP73 monoclonal antibodies was used to develop a sandwich immunity reaction using xMAP technology for the simultaneous detection of plasma AFP and GP73. The assay evaluated the sensitivity, cross reactivity, range of detection, precision, recovery and linearity dilution effect. The assay utilized plasma samples and compared its performance with commercially available Enzyme Linked Immunosorbent Assay (ELISA) kits. The assay was successful in detecting AFP and GP73 simultaneously. Validation experiments demonstrated the limit of detection was AFP 0.006 μg/l and GP73 0.482 μg/l. The functional sensitivity was AFP 0.010 μg/l and GP73 0.640 μg/l. The range of detection was AFP 0.01-50 μg/l and GP73 0.64-100 μg/l. No cross reactivity was observed. The intra- and inter-assay variation for AFP was 0.19-3.46% and 3.1-5.8% and for GP73 was 1.5-3.2% and 1.1-7.6% respectively. The recovery for AFP was 96-106% and GP73 was 89-110%. 80 clinical plasma samples from healthy controls, and patients with liver cirrhosis and Hepatocellular Carcinoma (HCC) were evaluated. For healthy controls (n = 25), the AFP was 2.40 (1.55, 3.30) μg/l and GP73 was 42.60 (39.10, 57.40) μg/l. For patients with liver cirrhosis (n = 19), the AFP was 2.60 (1.70, 4.20) μg/l and GP73 was 136.10 (92.10, 261.70) μg/l, and for HCC patients (n = 36), the AFP was 13.65 (3.35, 158.88) μg/l and GP73 was 186.25 (96.73, 262.03) μg/l. The new assay demonstrated a good correlation with commercially available ELISA kits (correlation coefficients (r) were 0.997 (AFP, p < 0.001) and 0.959 (GP73, p < 0.001). The method demonstrated a sensitive, effective and accurate method for the simultaneous detection of AFP and GP73, and could be used clinically for routine detection and monitoring of patients with chronic hepatitis B

Detection and Antigenic Profiling of Undeclared Peanut in Imported Garlic Using an xMAP Multiplex Immunoassay for Food Allergens.

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Pedersen, Ronnie O; Peters, Tim; Panda, Rakhi; Wehling, Paul; Garber, Eric A E

2017-07-01

A shipment of imported garlic powder was suspected of containing peanut. Samples (subs) collected from the shipment displayed considerable variability in peanut antigenicity when analyzed by enzyme-linked immunosorbent assay (ELISA). This raised questions regarding whether peanut was actually present, the amount present, and the basis for the variability in antigenic content. Analyses that used an xMAP multiplex assay for the detection of peanut and additional food allergens generated responses that were characteristic of peanut. Specifically, the relative intensities of two different peanut-specific antibodies coupled to beads (peanut-37 and -38) and the antigen profiles were identical to garlic controls spiked with peanut. In addition, the xMAP data did not indicate the presence of other allergens. Quantitative analyses indicated an approximately fivefold variation in peanut concentration among different subs. In contrast, within a sub, the apparent peanut concentration appeared constant. Particle size analyses of the garlic powder subs indicated a single distribution profile, with a peak at 380 μm. ELISA analysis of sieve-fractionated garlic powder from one of the subs indicated that slightly less than half of the detectable peanut was smaller than 212 μm, with the remainder almost evenly split between 212 and 300 μm and >300 μm. Modeling to predict possible oral exposure levels of peanut other than those directly measured requires additional research on the physicochemical properties of peanut and garlic, along with information on the production of the garlic powder.

Current status and future perspectives on molecular and serological methods in diagnostic mycology.

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Lau, Anna; Chen, Sharon; Sleiman, Sue; Sorrell, Tania

2009-11-01

Invasive fungal infections are an important cause of infectious morbidity. Nonculture-based methods are increasingly used for rapid, accurate diagnosis to improve patient outcomes. New and existing DNA amplification platforms have high sensitivity and specificity for direct detection and identification of fungi in clinical specimens. Since laboratories are increasingly reliant on DNA sequencing for fungal identification, measures to improve sequence interpretation should support validation of reference isolates and quality control in public gene repositories. Novel technologies (e.g., isothermal and PNA FISH methods), platforms enabling high-throughput analyses (e.g., DNA microarrays and Luminex xMAP) and/or commercial PCR assays warrant further evaluation for routine diagnostic use. Notwithstanding the advantages of molecular tests, serological assays remain clinically useful for patient management. The serum Aspergillus galactomannan test has been incorporated into diagnostic algorithms of invasive aspergillosis. Both the galactomannan and the serum beta-D-glucan test have value for diagnosing infection and monitoring therapeutic response.

Analysis of Luminex-based algorithms to define unacceptable HLA antibodies in CDC-crossmatch negative kidney transplant recipients.

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Zecher, Daniel; Bach, Christian; Preiss, Adrian; Staudner, Christoph; Utpatel, Kirsten; Evert, Matthias; Jung, Bettina; Bergler, Tobias; Böger, Carsten A; Spriewald, Bernd M; Banas, Bernhard

2018-02-20

HLA-specific antibodies detected by solid phase assays are increasingly used to define unacceptable HLA antigen mismatches (UAM) prior to renal transplantation. The accuracy of this approach is unclear. Day of transplant sera from 211 CDC-crossmatch-negative patients were retrospectively analyzed for donor-specific anti-HLA antibodies (DSA) using Luminex technology. HLA were defined as UAM if DSA had mean fluorescence intensity above (I) 3000 (patients retransplanted and those with DSA against HLA class I and II) or 5000 (all other patients), (II) 5000 for HLA A, B and DR and 10,000 for HLA DQ or (III) 10,000 (all HLA). We then studied the accuracy of these algorithms to identify patients with antibody-mediated rejection (AMR) and graft loss. UAM were also determined in 256 transplant candidates and virtual panel-reactive antibody (vPRA) levels calculated. At transplantation, 67/211 patients had DSA. Of these, 31 (algorithm I), 24 (II) and 17 (III) had UAM. 9 (I and II) and 8 (III) of 11 early AMR episodes and 7 (I), 6 (II) and 5 (III) of 9 graft losses occurred in UAM-positive patients during 4.9 years of follow-up. Algorithms (I) and (II) identified patients with persistently lower GFR even in the absence of overt AMR. 23-33% of waiting list patients had UAM with median vPRA of 69.2-79.1%. Algorithms (I) and (II) had comparable efficacy but were superior to (III) in identifying at-risk patients at an acceptable false positive rate. However, Luminex-defined UAM significantly restrict the donor pool of affected patients, which might prolong waiting time.

Development and validation of a Luminex assay for detection of a predictive biomarker for PROSTVAC-VF therapy

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Lucas, Julie L.; Tacheny, Erin A.; Ferris, Allison; Galusha, Michelle; Srivastava, Apurva K.; Ganguly, Aniruddha; Williams, P. Mickey; Sachs, Michael C.; Thurin, Magdalena; Tricoli, James V.; Ricker, Winnie; Gildersleeve, Jeffrey C.

2017-01-01

Cancer therapies can provide substantially improved survival in some patients while other seemingly similar patients receive little or no benefit. Strategies to identify patients likely to respond well to a given therapy could significantly improve health care outcomes by maximizing clinical benefits while reducing toxicities and adverse effects. Using a glycan microarray assay, we recently reported that pretreatment serum levels of IgM specific to blood group A trisaccharide (BG-Atri) correlate positively with overall survival of cancer patients on PROSTVAC-VF therapy. The results suggested anti-BG-Atri IgM measured prior to treatment could serve as a biomarker for identifying patients likely to benefit from PROSTVAC-VF. For continued development and clinical application of serum IgM specific to BG-Atri as a predictive biomarker, a clinical assay was needed. In this study, we developed and validated a Luminex-based clinical assay for measuring serum IgM specific to BG-Atri. IgM levels were measured with the Luminex assay and compared to levels measured using the microarray for 126 healthy individuals and 77 prostate cancer patients. This assay provided reproducible and consistent results with low %CVs, and tolerance ranges were established for the assay. IgM levels measured using the Luminex assay were found to be highly correlated to the microarray results with R values of 0.93–0.95. This assay is a Laboratory Developed Test (LDT) and is suitable for evaluating thousands of serum samples in CLIA certified laboratories that have validated the assay. In addition, the study demonstrates that discoveries made using neoglycoprotein-based microarrays can be readily migrated to a clinical assay. PMID:28771597

Development of a Sensitive and Specific Serological Assay Based on Luminex Technology for Detection of Antibodies to Zaire Ebola Virus.

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Ayouba, Ahidjo; Touré, Abdoulaye; Butel, Christelle; Keita, Alpha Kabinet; Binetruy, Florian; Sow, Mamadou S; Foulongne, Vincent; Delaporte, Eric; Peeters, Martine

2017-01-01

The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different viral regions (nucleoprotein [NP], 40-kDa viral protein [VP40], and glycoprotein [GP]) from four of the five EBV lineages were used. Samples from 94 survivors of the EBOV outbreak in Guinea and negative samples from 108 patients in France were used to calculate test performance for EBOV detection and cross-reaction with other Ebola virus lineages. For EBOV antibody detection, sensitivities of 95.7%, 96.8%, and 92.5% and specificities of 94.4%, 95.4%, and 96.3% for NP, GP, and VP40, respectively, were observed. All EBOV-negative samples that presented a reaction, except for one, interacted with a single antigen, whereas almost all samples from EBOV survivors were simultaneously reactive with NP and GP (90/94) or with NP, GP, and VP40 (87/94). Considering as positive for past EBOV infection only samples that reacted with EBOV NP and GP, sensitivity was 95.7% and specificity increased to 99.1%. Comparing results with commercial EBOV NP and GP enzyme-linked immunosorbent assays (ELISAs; Alpha Diagnostic, San Antonio, TX), lower sensitivity (92.5%) and high specificity (100%) were observed with the same positivity criteria. Samples from EBOV survivors cross-reacted with GP from Sudan Ebola virus (GP-SUDV) (81.9%), GP from Bundibugyo Ebola virus (GP-BDBV) (51.1%), GP from Reston Ebola virus (GP-RESTV) (9.6%), VP40-SUDV (76.6%), and VP40-BDBV (38.3%). Overall, we developed a sensitive and specific high-throughput serological assay, and defined an algorithm, for epidemiological surveys with humans. Copyright © 2016 American Society for Microbiology.

Rapid detection and semi-quantification of IgG-accessible Staphylococcus aureus surface-associated antigens using a multiplex competitive Luminex assay

NARCIS (Netherlands)

Hansenova Manaskova, S.; Bikker, F.J.; Veerman, E.C.I.; van Belkum, A.; van Wamel, W.J.B.

2013-01-01

The surface characterization of Staphylococcus aureus is currently labor intensive and time consuming. Therefore, we developed a novel method for the rapid yet comprehensive characterization of S. aureus cell-surface-associated proteins and carbohydrates, based on a competitive Luminex assay. In

The impact of pre-analytical variables on the stability of neurofilament proteins in CSF, determined by a novel validated SinglePlex Luminex assay and ELISA.

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Koel-Simmelink, Marleen J A; Vennegoor, Anke; Killestein, Joep; Blankenstein, Marinus A; Norgren, Niklas; Korth, Carsten; Teunissen, Charlotte E

2014-01-15

Neurofilament (Nf) proteins have been shown to be promising biomarkers for monitoring and predicting disease progression for various neurological diseases. The aim of this study was to evaluate the effects of pre-analytical variables on the concentration of neurofilament heavy (NfH) and neurofilament light (NfL) proteins. For NfH an in-house newly-developed and validated SinglePlex Luminex assay was used; ELISA was used to analyze NfL. For the NfL ELISA assay, the intra- and inter-assay variation was respectively, 1.5% and 16.7%. Analytical performance of the NfH SinglePlex Luminex assay in terms of sensitivity (6.6pg/mL), recovery in cerebrospinal fluid (CSF) (between 90 and 104%), linearity (from 6.6-1250pg/mL), and inter- and intra-assay variation (<8%) were good. Concentrations of both NfL and NfH appeared not negatively affected by blood contamination, repeated freeze-thaw cycles (up to 4), delayed processing (up to 24hours) and during long-term storage at -20°C, 4°C, and room temperature. A decrease in concentration was observed during storage of both neurofilament proteins up to 21days at 37°C, which was significant by day 5. The newly developed NfH SinglePlex Luminex assay has a good sensitivity and is robust. Moreover, both NfH and NfL are stable under the most prevalent pre-analytical variations. Copyright © 2013 Elsevier B.V. All rights reserved.

Detection of enteropathogens associated with travelers’ diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

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Lalani, Tahaniyat; Tisdale, Michele D; Maguire, Jason D; Wongsrichanalai, Chansuda; Riddle, Mark S; Tribble, David R

2015-01-01

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman FTA Elute cards smeared with stool containing pathogens associated with travelers’ diarrhea. LoDs ranged between 102-105 CFU, PFU or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoD increased with prolonged storage of cards. PMID:26072151

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

Science.gov (United States)

Libert, Xavier; Packeu, Ann; Bureau, Fabrice; Roosens, Nancy H; De Keersmaecker, Sigrid C J

2017-01-01

Considered as a public health problem, indoor fungal contamination is generally monitored using classical protocols based on culturing. However, this culture dependency could influence the representativeness of the fungal population detected in an analyzed sample as this includes the dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this study a Luminex xMAP® assay was developed for the simultaneous detection of 10 fungal species which are most frequently in indoor air and that may cause health problems. This xMAP® assay was found to be sensitive, i.e. its limit of detection is ranging between 0.05 and 0.01 ng of gDNA. The assay was subsequently tested with environmental air samples which were also analyzed with a classical protocol. All the species identified with the classical method were also detected with the xMAP® assay, however in a shorter time frame. These results demonstrate that the Luminex xMAP® fungal assay developed in this study could contribute to the improvement of public health and specifically to the indoor fungal contamination treatment.

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

Directory of Open Access Journals (Sweden)

Xavier Libert

Full Text Available Considered as a public health problem, indoor fungal contamination is generally monitored using classical protocols based on culturing. However, this culture dependency could influence the representativeness of the fungal population detected in an analyzed sample as this includes the dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this study a Luminex xMAP® assay was developed for the simultaneous detection of 10 fungal species which are most frequently in indoor air and that may cause health problems. This xMAP® assay was found to be sensitive, i.e. its limit of detection is ranging between 0.05 and 0.01 ng of gDNA. The assay was subsequently tested with environmental air samples which were also analyzed with a classical protocol. All the species identified with the classical method were also detected with the xMAP® assay, however in a shorter time frame. These results demonstrate that the Luminex xMAP® fungal assay developed in this study could contribute to the improvement of public health and specifically to the indoor fungal contamination treatment.

Amniotic fluid inflammatory cytokines: potential markers of immunologic dysfunction in autism spectrum disorders.

Science.gov (United States)

Abdallah, Morsi W; Larsen, Nanna; Grove, Jakob; Nørgaard-Pedersen, Bent; Thorsen, Poul; Mortensen, Erik L; Hougaard, David M

2013-09-01

The aim of the study was to analyze cytokine profiles in amniotic fluid (AF) samples of children developing autism spectrum disorders (ASD) and controls, adjusting for maternal autoimmune disorders and maternal infections during pregnancy. AF samples of 331 ASD cases and 698 controls were analyzed for inflammatory cytokines using Luminex xMAP technology utilizing a historic birth cohort. Clinical data were retrieved from nationwide registers, and case-control differences in AF cytokine levels were assessed using chi-square tests, logistic and tobit regression models. Overall, individuals with ASD had significantly elevated AF levels of TNF-α and TNF-β compared to controls. Analyzing individuals diagnosed only with ICD-10 codes yielded significantly elevated levels of IL-4, IL-10, TNF-α and TNF-β in ASD patients. Restricting analysis to infantile autism cases showed significantly elevated levels of IL-4, TNF-α and TNF-β compared to controls with no psychiatric comorbidities. Elevated levels of IL-6 and IL-5 were found in individuals with other childhood psychiatric disorders (OCPD) when compared to controls with no psychiatric comorbidities. AF samples of individuals with ASD or OCPD showed differential cytokine profiles compared to frequency-matched controls. Further studies to examine the specificity of the reported cytokine profiles in ASD and OCPD are required.

HPV genotyping and site of viral integration in cervical cancers in Indian women.

Directory of Open Access Journals (Sweden)

Poulami Das

Full Text Available Persistent HPV infection plays a major role in cervical cancer. This study was undertaken to identify HPV types in a cohort of Indian women with locally advanced cervical cancer as well as to determine the physical state and/or site of viral integration in the host genome. Pretreatment biopsies (n = 270 from patients were screened for HPV infection by a high throughput HPV genotyping assay based on luminex xMAP technology as well as MY09/11 PCR and SPF1/2 PCR. Overall HPV positivity was observed to be 95%, with HPV16 being most common (63% followed by infection with HPV18. Integration status of the virus was identified using Amplification of Papillomavirus Oncogene Transcripts (APOT assay in a subset of samples positive for HPV16 and/or HPV18 (n = 86 and with an adequate follow-up. The data was correlated with clinical outcome of the patients. Integration of the viral genome was observed in 79% of the cases and a preference for integration into the chromosomal loci 1p, 3q, 6q, 11q, 13q and 20q was seen. Clinical data revealed that the physical state of the virus (integrated or episomal could be an important prognostic marker for cervical cancer.

Rapid bead-based immunoassay for measurement of mannose-binding lectin

DEFF Research Database (Denmark)

Bay, J T; Garred, P

2009-01-01

have been developed more automated platforms for MBL analysis is urgently needed. To pursue this, we set out to develop a flexible bead-based MBL immunoassay. Serum was obtained from 98 healthy individuals and 50 patients investigated for possible immunodeficiencies. We used the Luminex xMAP bead array...... coefficient were found be 7.88% and 5.70%, respectively. A close correlation between the new assay and a reference MBL measurement ELISA was found (rho 0.9381, P bead-based assay was less sensitive to interfering anti-murine antibodies in the blood samples than when the antibodies employed were...... used in the reference polystyrene-based ELISA. The new assay could be performed in 3 h with less than 25 microl serum required of each sample. These results show that MBL can be measured readily using a bead-based platform, which may form an efficient basis for a multiplex approach to measure different...

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.

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Lalani, Tahaniyat; Tisdale, Michele D; Maguire, Jason D; Wongsrichanalai, Chansuda; Riddle, Mark S; Tribble, David R

2015-09-01

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman(™) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10(2) to 10(5)CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards. Copyright © 2015 Elsevier Inc. All rights reserved.

Development and customization of a color-coded microbeads-based assay for drug resistance in HIV-1 reverse transcriptase.

Science.gov (United States)

Gu, Lijun; Kawana-Tachikawa, Ai; Shiino, Teiichiro; Nakamura, Hitomi; Koga, Michiko; Kikuchi, Tadashi; Adachi, Eisuke; Koibuchi, Tomohiko; Ishida, Takaomi; Gao, George F; Matsushita, Masaki; Sugiura, Wataru; Iwamoto, Aikichi; Hosoya, Noriaki

2014-01-01

Drug resistance (DR) of HIV-1 can be examined genotypically or phenotypically. Although sequencing is the gold standard of the genotypic resistance testing (GRT), high-throughput GRT targeted to the codons responsible for DR may be more appropriate for epidemiological studies and public health research. We used a Japanese database to design and synthesize sequence-specific oligonucleotide probes (SSOP) for the detection of wild-type sequences and 6 DR mutations in the clade B HIV-1 reverse transcriptase region. We coupled SSOP to microbeads of the Luminex 100 xMAP system and developed a GRT based on the polymerase chain reaction (PCR)-SSOP-Luminex method. Sixteen oligoprobes for discriminating DR mutations from wild-type sequences at 6 loci were designed and synthesized, and their sensitivity and specificity were confirmed using isogenic plasmids. The PCR-SSOP-Luminex DR assay was then compared to direct sequencing using 74 plasma specimens from treatment-naïve patients or those on failing treatment. In the majority of specimens, the results of the PCR-SSOP-Luminex DR assay were concordant with sequencing results: 62/74 (83.8%) for M41, 43/74 (58.1%) for K65, 70/74 (94.6%) for K70, 55/73 (75.3%) for K103, 63/73 (86.3%) for M184 and 68/73 (93.2%) for T215. There were a number of specimens without any positive signals, especially for K65. The nucleotide position of A2723G, A2747G and C2750T were frequent polymorphisms for the wild-type amino acids K65, K66 and D67, respectively, and 14 specimens had the D67N mutation encoded by G2748A. We synthesized 14 additional oligoprobes for K65, and the sensitivity for K65 loci improved from 43/74 (58.1%) to 68/74 (91.9%). We developed a rapid high-throughput assay for clade B HIV-1 DR mutations, which could be customized by synthesizing oligoprobes suitable for the circulating viruses. The assay could be a useful tool especially for public health research in both resource-rich and resource-limited settings.

Plasma Biomarkers Discriminate Clinical Forms of Multiple Sclerosis

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Tejera-Alhambra, Marta; Casrouge, Armanda; de Andrés, Clara; Seyfferth, Ansgar; Ramos-Medina, Rocío; Alonso, Bárbara; Vega, Janet; Fernández-Paredes, Lidia; Albert, Matthew L.; Sánchez-Ramón, Silvia

2015-01-01

Multiple sclerosis, the most common cause of neurological disability in young population after trauma, represents a significant public health burden. Current challenges associated with management of multiple sclerosis (MS) patients stem from the lack of biomarkers that might enable stratification of the different clinical forms of MS and thus prompt treatment for those patients with progressive MS, for whom there is currently no therapy available. In the present work we analyzed a set of thirty different plasma cytokines, chemokines and growth factors present in circulation of 129 MS patients with different clinical forms (relapsing remitting, secondary progressive and primary progressive MS) and 53 healthy controls, across two independent cohorts. The set of plasma analytes was quantified with Luminex xMAP technology and their predictive power regarding clinical outcome was evaluated both individually using ROC curves and in combination using logistic regression analysis. Our results from two independent cohorts of MS patients demonstrate that the divergent clinical and histology-based MS forms are associated with distinct profiles of circulating plasma protein biomarkers, with distinct signatures being composed of chemokines and growth/angiogenic factors. With this work, we propose that an evaluation of a set of 4 circulating biomarkers (HGF, Eotaxin/CCL11, EGF and MIP-1β/CCL4) in MS patients might serve as an effective tool in the diagnosis and more personalized therapeutic targeting of MS patients. PMID:26039252

Pro-Inflammatory Cytokines Predict Relapse-Free Survival after One Month of Interferon-α but Not Observation in Intermediate Risk Melanoma Patients.

Directory of Open Access Journals (Sweden)

Ahmad A Tarhini

Full Text Available E1697 was a phase III trial of adjuvant interferon (IFN-α2b for one month (Arm B versus observation (Arm A in patients with resected melanoma at intermediate risk. We evaluated the levels of candidate serum cytokines, the HLA genotype, polymorphisms of CTLA4 and FOXP3 genes and the development of autoantibodies for their association with relapse free survival (RFS in Arm A and Arm B among 268 patients with banked biospecimens.ELISA was used to test 5 autoantibodies. Luminex/One Lambda LABTypeRSSO was used for HLA Genotyping. Selected CTLA4 and FOXP3 Single nucleotide polymorphisms (SNPs and microsatellites were tested for by polymerase chain reaction (PCR. Sixteen serum cytokines were tested at baseline and one month by Luminex xMAP multiplex technology. Cox Proportional Hazards model was applied and the Wald test was used to test the marginal association of each individual marker and RFS. We used the Lasso approach to select the markers to be included in a multi-marker Cox Proportional Hazards model. The ability of the resulting models to predict one year RFS was evaluated by the time-dependent ROC curve. The leave-one-out method of cross validation (LOOCV was used to avoid over-fitting of the data.In the multi-marker modeling analysis conducted in Arm B, one month serum IL2Rα, IL-12p40 and IFNα levels predicted one year RFS with LOOCV AUC = 82%. Among the three markers selected, IL2Rα and IFNα were the most stable (selected in all the cross validation cycles. The risk score (linear combination of the 3 markers separated the RFS curves of low and high risk groups well (p = 0.05. This model did not hold for Arm A, indicating a differential marker profile in Arm B linked to the intervention (adjuvant therapy.Early on-treatment proinflammatory serum markers (IL2Rα, IL-12p40, IFNα significantly predict RFS in our cohort of patients treated with adjuvant IFN-α2b and warrant further study.

Biological Threats Detection Technologies

International Nuclear Information System (INIS)

Bartoszcze, M.

2007-01-01

Among many decisive factors, which can have the influence on the possibility of decreases the results of use biological agents should be mentioned obligatory: rapid detection and identification of biological factor used, the proper preventive treatment and the medical management. The aims of identification: to identify the factor used, to estimate the area of contamination, to evaluate the possible countermeasure efforts (antibiotics, disinfectants) and to assess the effectiveness of the decontamination efforts (decontamination of the persons, equipment, buildings, environment etc.). The objects of identification are: bacteria and bacteria's spores, viruses, toxins and genetically modified factors. The present technologies are divided into: based on PCR techniques (ABI PRISM, APSIS, BIOVERIS, RAPID), immuno (BADD, RAMP, SMART) PCR and immuno techniques (APDS, LUMINEX) and others (BDS2, LUNASCAN, MALDI). The selected technologies assigned to field conditions, mobile and stationary laboratories will be presented.(author)

Development of a swine specific 9-plex Luminex cytokine assay and assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination: Elevated serum IL-12 levels are not predictive of protect

Science.gov (United States)

A Luminex multiplex swine cytokine assay was developed to measure 9 cytokines simultaneously in pig serum and tested in a porcine reproductive and respiratory syndrome virus (PRRSV) vaccine/challenge study. This assay detects innate (IL-1ß, IL-6, IL-8, IFNa, TNFa); regulatory (IL-10), Th1 (IL-12, I...

Identification of Brucella genus and eight Brucella species by Luminex bead-based suspension array.

Science.gov (United States)

Lusk Pfefer, Tina S; Timme, Ruth; Kase, Julie A

2018-04-01

Globally, unpasteurized milk products are vehicles for the transmission of brucellosis, a zoonosis responsible for cases of foodborne illness in the United States and elsewhere. Existing PCR assays to detect Brucella species are restricted by the resolution of band sizes on a gel or the number of fluorescent channels in a single real-time system. The Luminex bead-based suspension array is performed in a 96-well plate allowing for high throughput screening of up to 100 targets in one sample with easily discernible results. We have developed an array using the Bio-Plex 200 to differentiate the most common Brucella species: B. abortus, B. melitensis, B. suis, B. suis bv5, B. canis, B. ovis, B. pinnipedia, and B. neotomae, as well as Brucella genus. All probes showed high specificity, with no cross-reaction with non-Brucella strains. We could detect pure DNA from B. abortus, B. melitensis, and genus-level Brucella at concentrations of ≤5 fg/μL. Pure DNA from all other species tested positive at concentrations well below 500 fg/μL and we positively identified B. neotomae in six artificially contaminated cheese and milk products. An intra-laboratory verification further demonstrated the assay's accuracy and robustness in the rapid screening (3-4 h including PCR) of DNA. Published by Elsevier Ltd.

Biomarkers in the Diagnosis and Prognosis of Alzheimer's Disease.

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Schaffer, Cole; Sarad, Nakia; DeCrumpe, Ashton; Goswami, Disha; Herrmann, Sara; Morales, Jose; Patel, Parth; Osborne, Jim

2015-10-01

Alzheimer's disease (AD) is a neurodegenerative disease that inhibits cognitive functions and has no cure. This report reviews the current diagnostic standards for AD with an emphasis on early diagnosis using the cerebrospinal fluid (CSF) biomarkers amyloid-beta, t-tau, and p-tau and fluorodeoxyglucose positron emission tomography imaging. Abnormal levels of these CSF biomarkers and decreased cerebral uptake of glucose have recently been used in the early diagnosis of AD in experimental studies. These promising biomarkers can be measured using immunoassays performed in singleplex or multiplex formats. Although presently, there are no Food and Drug Administration-approved in vitro diagnostics (IVDs) for early detection of AD, a multiplex immunoassay measuring a panel of promising AD biomarkers in CSF may be a likely IVD candidate for the clinical AD diagnostic market. Specifically, the INNO-BIA AlzBio3 immunoassay kit, performed using bead arrays on the xMAP Luminex analyzer, allows simultaneous quantification of amyloid-beta, t-tau, and p-tau biomarkers. AD biomarkers can also be screened using enzyme-linked immunosorbent assays that are offered as laboratory-developed tests. © 2014 Society for Laboratory Automation and Screening.

Upconversion Nanoparticles-Encoded Hydrogel Microbeads-Based Multiplexed Protein Detection

Science.gov (United States)

Shikha, Swati; Zheng, Xiang; Zhang, Yong

2018-06-01

Fluorescently encoded microbeads are in demand for multiplexed applications in different fields. Compared to organic dye-based commercially available Luminex's xMAP technology, upconversion nanoparticles (UCNPs) are better alternatives due to their large anti-Stokes shift, photostability, nil background, and single wavelength excitation. Here, we developed a new multiplexed detection system using UCNPs for encoding poly(ethylene glycol) diacrylate (PEGDA) microbeads as well as for labeling reporter antibody. However, to prepare UCNPs-encoded microbeads, currently used swelling-based encapsulation leads to non-uniformity, which is undesirable for fluorescence-based multiplexing. Hence, we utilized droplet microfluidics to obtain encoded microbeads of uniform size, shape, and UCNPs distribution inside. Additionally, PEGDA microbeads lack functionality for probe antibodies conjugation on their surface. Methods to functionalize the surface of PEGDA microbeads (acrylic acid incorporation, polydopamine coating) reported thus far quench the fluorescence of UCNPs. Here, PEGDA microbeads surface was coated with silica followed by carboxyl modification without compromising the fluorescence intensity of UCNPs. In this study, droplet microfluidics-assisted UCNPs-encoded microbeads of uniform shape, size, and fluorescence were prepared. Multiple color codes were generated by mixing UCNPs emitting red and green colors at different ratios prior to encapsulation. UCNPs emitting blue color were used to label the reporter antibody. Probe antibodies were covalently immobilized on red UCNPs-encoded microbeads for specific capture of human serum albumin (HSA) as a model protein. The system was also demonstrated for multiplexed detection of both human C-reactive protein (hCRP) and HSA protein by immobilizing anti-hCRP antibodies on green UCNPs.

Comparison of Multiplex Suspension Array Large-Panel Kits for Profiling Cytokines and Chemokines in Rheumatoid Arthritis Patients

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Khan, Imran H.; Krishnan, V.V.; Ziman, Melanie; Janatpour, Kim; Wun, Ted; Luciw, Paul A.; Tuscano, Joseph

2015-01-01

Background Multiplex analysis allows measurements of a large number of analytes simultaneously in each sample. Based on the Luminex multiplex technology (xMAP), kits for measuring multiple cytokines and chemokines (immunomodulators) are commercially available and are useful in investigations on inflammatory diseases. This study evaluated four multiplex kits (Bio-Plex, LINCOplex, Fluorokine, and Beadlyte) that contained 27, 29, 20 and 22 analytes each, respectively, for the analysis of immunomodulators in plasma of rheumatoid arthritis (RA) patients who underwent treatment with antibody against CD20 (rituximab), a B-cell reductive therapy. Methods Multiplex kits were tested on serial plasma samples obtained from six RA patients at baseline and multiple time points (3, 6, and 9 months) post-treatment with rituximab. The RA patients included in this study had previously failed therapy with disease modifying anti-arthritis drugs (DMARD) and treatment with anti-TNFα antibody (infliximab). Results Computer modeling and hierarchical cluster analysis of the multiplex data allowed a comparison of the performance of multiplex assay kits and revealed profiles of immunomodulators in the RA patients. Conclusions In plasma of RA patients who appeared to have benefited from rituximab treatment the profile of significantly elevated immunomodulators by at least two of the three kits (BioPlex, LINCOplex, Beadlyte), is as follows: IL-12p70, Eotaxin, IL-4, TNFα, Il-9, IL-1β, IFNγ, IL-10, IL-6, and IL-13. Immunomodulator profiling by multiplex analysis may provide useful plasma biomarkers for monitoring response to B-cell reductive therapy in RA patients. PMID:18823005

Regulation of microtubule-based transport by MAP4

Science.gov (United States)

Semenova, Irina; Ikeda, Kazuho; Resaul, Karim; Kraikivski, Pavel; Aguiar, Mike; Gygi, Steven; Zaliapin, Ilya; Cowan, Ann; Rodionov, Vladimir

2014-01-01

Microtubule (MT)-based transport of organelles driven by the opposing MT motors kinesins and dynein is tightly regulated in cells, but the underlying molecular mechanisms remain largely unknown. Here we tested the regulation of MT transport by the ubiquitous protein MAP4 using Xenopus melanophores as an experimental system. In these cells, pigment granules (melanosomes) move along MTs to the cell center (aggregation) or to the periphery (dispersion) by means of cytoplasmic dynein and kinesin-2, respectively. We found that aggregation signals induced phosphorylation of threonine residues in the MT-binding domain of the Xenopus MAP4 (XMAP4), thus decreasing binding of this protein to MTs. Overexpression of XMAP4 inhibited pigment aggregation by shortening dynein-dependent MT runs of melanosomes, whereas removal of XMAP4 from MTs reduced the length of kinesin-2–dependent runs and suppressed pigment dispersion. We hypothesize that binding of XMAP4 to MTs negatively regulates dynein-dependent movement of melanosomes and positively regulates kinesin-2–based movement. Phosphorylation during pigment aggregation reduces binding of XMAP4 to MTs, thus increasing dynein-dependent and decreasing kinesin-2–dependent motility of melanosomes, which stimulates their accumulation in the cell center, whereas dephosphorylation of XMAP4 during dispersion has an opposite effect. PMID:25143402

Comparison of pre-processing methods for multiplex bead-based immunoassays.

Science.gov (United States)

Rausch, Tanja K; Schillert, Arne; Ziegler, Andreas; Lüking, Angelika; Zucht, Hans-Dieter; Schulz-Knappe, Peter

2016-08-11

High throughput protein expression studies can be performed using bead-based protein immunoassays, such as the Luminex® xMAP® technology. Technical variability is inherent to these experiments and may lead to systematic bias and reduced power. To reduce technical variability, data pre-processing is performed. However, no recommendations exist for the pre-processing of Luminex® xMAP® data. We compared 37 different data pre-processing combinations of transformation and normalization methods in 42 samples on 384 analytes obtained from a multiplex immunoassay based on the Luminex® xMAP® technology. We evaluated the performance of each pre-processing approach with 6 different performance criteria. Three performance criteria were plots. All plots were evaluated by 15 independent and blinded readers. Four different combinations of transformation and normalization methods performed well as pre-processing procedure for this bead-based protein immunoassay. The following combinations of transformation and normalization were suitable for pre-processing Luminex® xMAP® data in this study: weighted Box-Cox followed by quantile or robust spline normalization (rsn), asinh transformation followed by loess normalization and Box-Cox followed by rsn.

Establishment of a Molecular Serotyping Scheme and a Multiplexed Luminex-Based Array for Enterobacter aerogenes.

Science.gov (United States)

Guo, Xi; Wang, Min; Wang, Lu; Wang, Yao; Chen, Tingting; Wu, Pan; Chen, Min; Liu, Bin; Feng, Lu

2018-01-01

Serotyping based on surface polysaccharide antigens is important for the clinical detection and epidemiological surveillance of pathogens. Polysaccharide gene clusters (PSgcs) are typically responsible for the diversity of bacterial surface polysaccharides. Through whole-genome sequencing and analysis, eight putative PSgc types were identified in 23 Enterobacter aerogenes strains from several geographic areas, allowing us to present the first molecular serotyping system for E. aerogenes . A conventional antigenic scheme was also established and correlated well with the molecular serotyping system that was based on PSgc genetic variation, indicating that PSgc-based molecular typing and immunological serology provide equally valid results. Further, a multiplex Luminex-based array was developed, and a double-blind test was conducted with 97 clinical specimens from Shanghai, China, to validate our array. The results of these analyses indicated that strains containing PSgc4 and PSgc7 comprised the predominant groups. We then examined 86 publicly available E. aerogenes strain genomes and identified an additional seven novel PSgc types, with PSgc10 being the most abundant type. In total, our study identified 15 PSgc types in E. aerogenes , providing the basis for a molecular serotyping scheme. From these results, differing epidemic patterns were identified between strains that were predominant in different regions. Our study highlights the feasibility and reliability of a serotyping system based on PSgc diversity, and for the first time, presents a molecular serotyping system, as well as an antigenic scheme for E. aerogenes , providing the basis for molecular diagnostics and epidemiological surveillance of this important emerging pathogen.

European week of science and technology - Webcast: Couldn't be without it!

CERN Multimedia

2002-01-01

Could you imagine life without objects such as mobile phones, cars, refrigerators, or antibiotics? Technology is everywhere and used by everyone in today's society, but most Europeans do not realise that most things they couldn't be without have come about through years of fundamental research. To fill this knowledge gap, seven leading Research Organizations*in Europe, including CERN, with the support of the research directorate of the European Commission, joined forces to inform Europeans how technology couldn't be without science. Sci-Tech - Couldn't be without it! is a website inaugurated in March that shows where the top technologies really come from, and it reveals their intimate links with research. Next week there will be three informative and entertaining webcasts (in English, French and Italian), broadcast live on the web from a studio at CERN. During these Webcasts scientists from the seven research Organizations and their industrial partners Sun Microsystems, Siemens, L'Oréal and Luminex will en...

Donor-Specific Anti-HLA Antibodies in Huntington's Disease Recipients of Human Fetal Striatal Grafts.

Science.gov (United States)

Porfirio, Berardino; Paganini, Marco; Mazzanti, Benedetta; Bagnoli, Silvia; Bucciantini, Sandra; Ghelli, Elena; Nacmias, Benedetta; Putignano, Anna Laura; Rombolà, Giovanni; Saccardi, Riccardo; Lombardini, Letizia; Di Lorenzo, Nicola; Vannelli, Gabriella B; Gallina, Pasquale

2015-01-01

Fetal grafting in a human diseased brain was thought to be less immunogenic than other solid organ transplants, hence the minor impact on the efficacy of the transplant. How much prophylactic immune protection is required for neural allotransplantation is also debated. High-sensitive anti-HLA antibody screening in this field has never been reported. Sixteen patients with Huntington's disease underwent human fetal striatal transplantation in the frame of an open-label observational trial, which is being carried out at Florence University. All patients had both brain hemispheres grafted in two separate robotic-stereotactic procedures. The trial started in February 2006 with the first graft to the first patient (R1). R16 was given his second graft on March 2011. All patients received triple immunosuppressive treatment. Pre- and posttransplant sera were analyzed for the presence of anti-HLA antibodies using the multiplexed microsphere-based suspension array Luminex xMAP technology. Median follow-up was 38.5 months (range 13-85). Six patients developed anti-HLA antibodies, which turned out to be donor specific. Alloimmunization occurred in a time window of 0-49 months after the first neurosurgical procedure. The immunogenic determinants were non-self-epitopes from mismatched HLA antigens. These determinants were both public epitopes shared by two or more HLA molecules and private epitopes unique to individual HLA molecules. One patient had non-donor-specific anti-HLA antibodies in her pretransplant serum sample, possibly due to previous sensitization events. Although the clinical significance of donor-specific antibodies is far from being established, particularly in the setting of neuronal transplantation, these findings underline the need of careful pre- and posttransplant immunogenetic evaluation of patients with intracerebral grafts.

Impact of 6-month frozen storage of cervical specimens in alkaline buffer conditions on human papillomavirus genotyping.

Science.gov (United States)

LaMere, Brandon J; Howell, Renee; Fetterman, Barbara; Shieh, Jen; Castle, Philip E

2008-08-01

The impact of 6-month storage of cervical specimens under alkaline conditions that occurs as the result of Hybrid Capture 2 testing on human papillomavirus (HPV) genotyping is not well documented. To examine this issue, 143 frozen hc2-positive specimens in specimen transport medium were selected at random from each of the following groups: specimens stored for 6 months, 4 months, and 2.5 months under alkaline pH (pH 12-13) and specimens stored 1 month at neutral pH (pH 6-7) as controls. Specimens were tested in a masked fashion for 20 HPV genotypes (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) using a prototype, research-use-only GP5+/6+ L1 consensus PCR method and multiplex hybridization using Luminex xMAP for detection of specific HPV genotypes One control specimen had missing test results. There were no statistical differences in the number of HPV genotypes detected, number of carcinogenic HPV genotypes detected, or in the signal strength among HPV-positive results across groups. Six-month frozen storage of cervical specimens at alkaline pH had little impact on testing for HPV genotypes among hc2-positive women using this HPV genotyping method.

Laboratory Tests of Multiplex Detection of PCR Amplicons Using the Luminex 100 Flow Analyzer

Energy Technology Data Exchange (ETDEWEB)

Venkateswaran, K.S.; Nasarabadi, S.; Langlois, R.G.

2000-05-05

Lawrence Livermore National Laboratory (LLNL) demonstrated the power of flow cytometry in detecting the biological agents simulants at JFT III. LLNL pioneered in the development of advanced nucleic acid analyzer (ANM) for portable real time identification. Recent advances in flow cytometry provide a means for multiplexed nucleic acid detection and immunoassay of pathogenic microorganisms. We are presently developing multiplexed immunoassays for the simultaneous detection of different simulants. Our goal is to build an integrated instrument for both nucleic acid analysis and immuno detection. In this study we evaluated the Luminex LX 100 for concurrent identification of more than one PCR amplified product. ANAA has real-time Taqman fluorescent detection capability for rapid identification of field samples. However, its multiplexing ability is limited by the combination of available fluorescent labels. Hence integration of ANAA with flow cytometry can give the rapidity of ANAA amplification and the multiplex capability of flow cytometry. Multiplexed flow cytometric analysis is made possible using a set of fluorescent latex microsphere that are individually identified by their red and infrared fluorescence. A green fluorochrome is used as the assay signal. Methods were developed for the identification of specific nucleic acid sequences from Bacillus globigii (Bg), Bacillus thuringensis (Bt) and Erwinia herbicola (Eh). Detection sensitivity using different reporter fluorochromes was tested with the LX 100, and also different assay formats were evaluated for their suitability for rapid testing. A blind laboratory trial was carried out December 22-27, 1999 to evaluate bead assays for multiplex identification of Bg and Bt PCR products. This report summarizes the assay development, fluorochrome comparisons, and the results of the blind trial conducted at LLNL for the laboratory evaluation of the LX 100 flow analyzer.

LEVELS OF ANGIOGENESIS-REGULATORY CHEMOKINES IN THE SYNOVIAL FLUID OF PATIENTS WITH RHEUMATOID ARTHRITIS

Directory of Open Access Journals (Sweden)

D. A. Zhebrun

2015-01-01

Full Text Available The role of chemokines in the immunopathogenesis of rheumatoid arthritis (RA has been actively investigated in recent years. Angiogenic and angiostatic chemokines are important mediators of angiogenesis in the development and extent of pannus. Peripheral blood and synovial fluid (SF is a major biomaterial in clinical and immunological studies. At the same time, it is the SF test that may yield the most informative results since that gives an idea of the processes that occur locally within a joint. Objective: to perform a comparative analysis of the levels of a number of CXC, CC, and CX3C chemokines in the SF of patients with RA, osteoarthritis (OA, and joint injuries. Subjects and methods. The multiplex analysis using xMAP technology (Luminex, USA was used to analyze levels of CXC, CC, and CX3C chemokines in SF and serum of patients with RA (n = 20, OA (n = 9 and controls (n = 9. Results and discussion. The SF levels of CCL24/eotaxin-2, as well as those of the angiostatic chemokines CXCL9/MIG, CXCL10/IP10, CXCL11/ITAC, and CXCL13/BCA-1 were higher in the RA group than in the control and OA groups. There was a direct correlation between SF levels of CCL5/RANTES and DAS28, as well as patient global disease activity assessment on visual analogue scale, and that between the level of CCL2/MCP-1 in the SF and that of anticyclic citrullinated peptide (anti-CCP antibodies in the serum. The SF concentrations of CXCL5/ENA78 and CXCL7/NAP-2 were shown to depend on the presence of serum anti-CCP. Serum CXCL13/BCA-1 levels were higher in RA than those in OA, as that of CXCL7/NAP-2 than in the control group.

Magnetic bead based immuno-detection of Listeria monocytogenes and Listeria ivanovii from infant formula and leafy green vegetables using the Bio-Plex suspension array system.

Science.gov (United States)

Day, J B; Basavanna, U

2015-04-01

Listeriosis, a disease contracted via the consumption of foods contaminated with pathogenic Listeria species, can produce severe symptoms and high mortality in susceptible people and animals. The development of molecular methods and immuno-based techniques for detection of pathogenic Listeria in foods has been challenging due to the presence of assay inhibiting food components. In this study, we utilize a macrophage cell culture system for the isolation and enrichment of Listeria monocytogenes and Listeria ivanovii from infant formula and leafy green vegetables for subsequent identification using the Luminex xMAP technique. Macrophage monolayers were exposed to infant formula, lettuce and celery contaminated with L. monocytogenes or L. ivanovii. Magnetic microspheres conjugated to Listeria specific antibody were used to capture Listeria from infected macrophages and then analyzed using the Bio-Plex 200 analyzer. As few as 10 CFU/mL or g of L. monocytogenes was detected in all foods tested. The detection limit for L. ivanovii was 10 CFU/mL in infant formula and 100 CFU/g in leafy greens. Microsphere bound Listeria obtained from infected macrophage lysates could also be isolated on selective media for subsequent confirmatory identification. This method presumptively identifies L. monocytogenes and L. ivanovii from infant formula, lettuce and celery in less than 28 h with confirmatory identifications completed in less than 48 h. Published by Elsevier Ltd.

New miRNA labeling method for bead-based quantification

Directory of Open Access Journals (Sweden)

Lanfranchi Gerolamo

2010-06-01

Full Text Available Abstract Background microRNAs (miRNAs are small single-stranded non-coding RNAs that act as crucial regulators of gene expression. Different methods have been developed for miRNA expression profiling in order to better understand gene regulation in normal and pathological conditions. miRNAs expression values obtained from large scale methodologies such as microarrays still need a validation step with alternative technologies. Results Here we have applied with an innovative approach, the Luminex® xMAP™ technology validate expression data of differentially expressed miRNAs obtained from high throughput arrays. We have developed a novel labeling system of small RNA molecules (below 200 nt, optimizing the sensitive cloning method for miRNAs, termed miRNA amplification profiling (mRAP. The Luminex expression patterns of three miRNAs (miR-23a, miR-27a and miR-199a in seven different cell lines have been validated by TaqMan miRNA assay. In all cases, bead-based meas were confirmed by the data obtained by TaqMan and microarray technologies. Conclusions We demonstrate that the measure of individual miRNA by the bead-based method is feasible, high speed, sensitive and low cost. The Luminex® xMAP™ technology also provides flexibility, since the central reaction can be scaled up with additional miRNA capturing beads, allowing validation of many differentially expressed miRNAs obtained from microarrays in a single experiment. We propose this technology as an alternative method to qRT-PCR for validating miRNAs expression data obtained with high-throughput technologies.

Amniotic fluid protein profiles of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria.

Science.gov (United States)

Kacerovsky, Marian; Celec, Peter; Vlkova, Barbora; Skogstrand, Kristin; Hougaard, David M; Cobo, Teresa; Jacobsson, Bo

2013-01-01

This study aimed to evaluate the amniotic fluid protein profiles and the intensity of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria, using the multiplex xMAP technology. A retrospective cohort study was undertaken in the Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Czech Republic. A total of 145 pregnant women with preterm prelabor rupture of membranes between gestational age 24+0 and 36+6 weeks were included in the study. Amniocenteses were performed. The presence of Ureaplasma spp. and other bacteria was evaluated using 16S rRNA gene sequencing. The levels of specific proteins were determined using multiplex xMAP technology. The presence of Ureaplasma spp. and other bacteria in the amniotic fluid was associated with increased levels of interleukin (IL)-6, IL-8, IL-10, brain-derived neurotropic factor, granulocyte macrophage colony stimulating factor, monocyte chemotactic protein-1, macrophage inflammatory protein-1, and matrix metalloproteinasis-9. Ureaplasma spp. were also associated with increased levels of neurotropin-3 and triggering receptor expressed on myeloid cells-1. The presence of Ureaplasma spp. in the amniotic fluid is associated with a slightly different protein profile of inflammatory response, but the intensity of inflammatory response to Ureaplasma spp. is comparable with the inflammatory response to other bacteria.

Standardization of a cytometric p24-capture bead-assay for the detection of main HIV-1 subtypes.

Science.gov (United States)

Merbah, Mélanie; Onkar, Sayali; Grivel, Jean-Charles; Vanpouille, Christophe; Biancotto, Angélique; Bonar, Lydia; Sanders-Buell, Eric; Kijak, Gustavo; Michael, Nelson; Robb, Merlin; Kim, Jerome H; Tovanabutra, Sodsai; Chenine, Agnès-Laurence

2016-04-01

The prevailing method to assess HIV-1 replication and infectivity is to measure the production of p24 Gag protein by enzyme-linked immunosorbent assay (ELISA). Since fluorescent bead-based technologies offer a broader dynamic range and higher sensitivity, this study describes a p24 capture Luminex assay capable of detecting HIV-1 subtypes A-D, circulating recombinant forms (CRF) CRF01_AE and CRF02_AG, which together are responsible for over 90% of HIV-1 infections worldwide. The success of the assay lies in the identification and selection of a cross-reactive capture antibody (clone 183-H12-5C). Fifty-six isolates that belonged to six HIV-1 subtypes and CRFs were successfully detected with p-values below 0.021; limits of detection ranging from 3.7 to 3 × 104 pg/ml. The intra- and inter-assay variation gave coefficient of variations below 6 and 14%, respectively. The 183-bead Luminex assay also displayed higher sensitivity of 91% and 98% compared to commercial p24 ELISA and a previously described Luminex assay. The p24 concentrations measured by the 183-bead Luminex assay showed a significant correlation (R=0.92, passay leverages the advantages of the Luminex platform, which include smaller sample volume and simultaneous detection of up to 500 analytes in a single sample, and delivers a valuable tool for the field. Copyright © 2016 Elsevier B.V. All rights reserved.

The performance of Luminex ARIES® Flu A/B & RSV and Cepheid Xpert® Flu/RSV XC for the detection of influenza A, influenza B, and respiratory syncytial virus in prospective patient samples.

Science.gov (United States)

McMullen, Phillip; Boonlayangoor, Sue; Charnot-Katsikas, Angella; Beavis, Kathleen G; Tesic, Vera

2017-10-01

The demand for rapid, accurate viral testing has increased the number of assays available for the detection of viral pathogens. One of the newest FDA cleared platforms is the Luminex ARIES ® Flu A/B & RSV, which is a fully automated, real-time PCR-based assay used for detection of influenza A, influenza B, and respiratory syncytial virus (RSV). We sought to compare the performance of Luminex ARIES ® Flu A/B & RSV assay to the Cepheid Xpert ® Flu/RSV XC assay for rapid Flu and RSV testing. A series of consecutive nasopharyngeal specimens received in the clinical microbiology laboratory during peak influenza season at a major academic center in Chicago, IL, were prospectively tested, using both the ARIES ® Flu A/B & RSV and Xpert ® Flu/RSV XC assays, side by side. Discrepant results were tested on the BioFire FilmArray ® Respiratory Panel for resolution. A total of 143 consecutive nasopharyngeal specimens, obtained from patients ranging from six months to ninety-three years in age were received between January 1st, 2017 and March 21st, 2017. There was 96.6% agreement between the two assays for detection influenza A, 100% agreement for detection influenza B and RSV, and 98.9% agreement for negative results. The Xpert ® Flu/RSV XC performed with an average turn-around time of approximately 60min, compared to the ARIES ® Flu A/B & RSV of approximately 120min. Both assays were equally easy to perform, with a similar amount of hands-on technologist time for each platform. Overall, these results indicate that both tests are comparable in terms of result agreement and technical ease-of-use. The Xpert ® Flu/RSV XC assay did produce results with less turn-around-time, approximately 60min quicker than the ARIES ® Flu A/B & RSV. Copyright © 2017 Elsevier B.V. All rights reserved.

Optical Encoding Technology for Viral Screening Panels Final Report CRADA No TC02132.0

Energy Technology Data Exchange (ETDEWEB)

Lenhoff, R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Haushalter, R. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

2017-08-15

This was a collaborative effort between Lawrence Livermore National Security, LLC, Lawrence Livermore National Laboratory (LLNL) and Parallel Synthesis Technologies, Inc. (PSTI), to develop Optical Encoding Technology for Viral Screening Panels. The goal for this effort was to prepare a portable bead reader system that would enable the development of viral and bacterial screening panels which could be used for the detection of any desired set of bacteria or viruses in any location. The main objective was to determine if the combination of a bead-based, PCR suspension array technology, formulated from Parallume encoded beads and PSTI’s multiplex assay reader system (MARS), could provide advantages in terms of the number of simultaneously measured samples, portability, ruggedness, ease of use, accuracy, precision or cost as compared to the Luminexbased system developed at LLNL. The project underwent several no cost extensions however the overall goal of demonstrating the utility of this new system was achieved. As a result of the project a significant change to the type of bead PSTI used for the suspension system was implemented allowing better performance than the commercial Luminex system.

[Investigation of mechanisms of action of growth factors of autologous platelet-rich plasma used to treat erectile dysfunction].

Science.gov (United States)

Epifanova, M V; Chalyi, M E; Krasnov, A O

2017-09-01

To determine the quantitative and qualitative composition of growth factors (PDGF-AA, PDGF-BB, VEGF, VEGF-D, FGF-acid, FGF-basic) and platelets in various modifications of APRP. Blood of 12 male volunteers (control group) and 12 patients with ED was used to prepare APRP and the subsequently determine the concentration of growth factors. The growth factor concentrations (FGF acid, FGF basic, PDGF-AA, PDGF-BB, VEGF, VEGF-D) was determined using a flow cytometry-based xMAP Luminex (Gen-Probe) system. Concentration of platelets in APRP obtained by two stage centrifugation, reached 1480 (1120-1644) in the control group and 1232 (956-1502) in patients with ED. The concentration of growth factors in the samples prepared without preliminary freezing was: PDGF-AA 842 (22-3700), PDGF-BB 2837 (1460-4100), FGF-basic 7.9 (0.28-127), FGF-acid 3, 4 (0.14-11), VEGF 19 (4.6-46), VEGF-D 21 (14-38). After thawing, the concentration of all growth factors in the samples increased. The study findings suggest that the mechanism of erectile function recovery following the use of APRP is through the active substances detected in APRP, i.e. FGF-basic, PDGF-AA, PDGF-BB, VEGF, VEGF-D and FGF-acid. Also, the study showed that the content of growth factors in APRP after of freezing/thawing is higher than in APRP that has not been frozen. This is due to the cell membrane destruction at extremely low temperatures during freezing.

Data mining strategies to improve multiplex microbead immunoassay tolerance in a mouse model of infectious diseases.

Directory of Open Access Journals (Sweden)

Akshay Mani

Full Text Available Multiplex methodologies, especially those with high-throughput capabilities generate large volumes of data. Accumulation of such data (e.g., genomics, proteomics, metabolomics etc. is fast becoming more common and thus requires the development and implementation of effective data mining strategies designed for biological and clinical applications. Multiplex microbead immunoassay (MMIA, on xMAP or MagPix platform (Luminex, which is amenable to automation, offers a major advantage over conventional methods such as Western blot or ELISA, for increasing the efficiencies in serodiagnosis of infectious diseases. MMIA allows detection of antibodies and/or antigens efficiently for a wide range of infectious agents simultaneously in host blood samples, in one reaction vessel. In the process, MMIA generates large volumes of data. In this report we demonstrate the application of data mining tools on how the inherent large volume data can improve the assay tolerance (measured in terms of sensitivity and specificity by analysis of experimental data accumulated over a span of two years. The combination of prior knowledge with machine learning tools provides an efficient approach to improve the diagnostic power of the assay in a continuous basis. Furthermore, this study provides an in-depth knowledge base to study pathological trends of infectious agents in mouse colonies on a multivariate scale. Data mining techniques using serodetection of infections in mice, developed in this study, can be used as a general model for more complex applications in epidemiology and clinical translational research.

Increased inflammatory response in cytomegalovirus seropositive patients with Alzheimer's disease.

Directory of Open Access Journals (Sweden)

Gabriel Westman

Full Text Available Alzheimer's disease (AD has been associated with increased local inflammation in the affected brain regions, and in some studies also with elevated levels of proinflammatory cytokines in peripheral blood. Cytomegalovirus (CMV is known to promote a more effector-oriented phenotype in the T-cell compartment, increasing with age. The aim of this study was to investigate the inflammatory response of peripheral blood mononuclear cells (PBMCs from AD patients and non-demented (ND controls. Using a multiplex Luminex xMAP assay targeting GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IP-10 and TNF-α, cytokine profiles from PBMCs were analysed after stimulation with anti-CD3/CD28 beads, CMV pp65 peptide mix or amyloid β (Aβ protofibrils, respectively. CMV seropositive AD subjects presented with higher IFN-γ levels after anti-CD3/CD28 and CMV pp65 but not after Aβ stimulation, compared to CMV seropositive ND controls. When analysing IFN-γ response to anti-CD3/CD28 stimulation on a subgroup level, CMV seropositive AD subjects presented with higher levels compared to both CMV seronegative AD and CMV seropositive ND subjects. Taken together, our data from patients with clinically manifest AD suggest a possible role of CMV as an inflammatory promoter in AD immunology. Further studies of AD patients at earlier stages of disease, could provide better insight into the pathophysiology.

Mycobacteria-specific cytokine responses as correlates of treatment response in active and latent tuberculosis.

Science.gov (United States)

Clifford, Vanessa; Tebruegge, Marc; Zufferey, Christel; Germano, Susie; Forbes, Ben; Cosentino, Lucy; McBryde, Emma; Eisen, Damon; Robins-Browne, Roy; Street, Alan; Denholm, Justin; Curtis, Nigel

2017-08-01

A biomarker indicating successful tuberculosis (TB) therapy would assist in determining appropriate length of treatment. This study aimed to determine changes in mycobacteria-specific antigen-induced cytokine biomarkers in patients receiving therapy for latent or active TB, to identify biomarkers potentially correlating with treatment success. A total of 33 adults with active TB and 36 with latent TB were followed longitudinally over therapy. Whole blood stimulation assays using mycobacteria-specific antigens (CFP-10, ESAT-6, PPD) were done on samples obtained at 0, 1, 3, 6 and 9 months. Cytokine responses (IFN-γ, IL-1ra, IL-2, IL-10, IL-13, IP-10, MIP-1β, and TNF-α) in supernatants were measured by Luminex xMAP immunoassay. In active TB cases, median IL-1ra (with CFP-10 and with PPD stimulation), IP-10 (CFP-10, ESAT-6), MIP-1β (ESAT-6, PPD), and TNF-α (ESAT-6) responses declined significantly over the course of therapy. In latent TB cases, median IL-1ra (CFP-10, ESAT-6, PPD), IL-2 (CFP-10, ESAT-6), and IP-10 (CFP-10, ESAT-6) responses declined significantly. Mycobacteria-specific cytokine responses change significantly over the course of therapy, and their kinetics in active TB differ from those observed in latent TB. In particular, mycobacteria-specific IL-1ra responses are potential correlates of successful therapy in both active and latent TB. Copyright © 2017 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

Development of a Luminex Bead Based Assay for Diagnosis of Toxocariasis Using Recombinant Antigens Tc-CTL-1 and Tc-TES-26.

Directory of Open Access Journals (Sweden)

John P Anderson

Full Text Available The clinical spectrum of human disease caused by the roundworms Toxocara canis and Toxocara cati ranges from visceral and ocular larva migrans to covert toxocariasis. The parasite is not typically recovered in affected tissues, so detection of parasite-specific antibodies is usually necessary for establishing a diagnosis. The most reliable immunodiagnostic methods use the Toxocara excretory-secretory antigens (TES-Ag in ELISA formats to detect Toxocara-specific antibodies. To eliminate the need for native parasite materials, we identified and purified immunodiagnostic antigens using 2D gel electrophoresis followed by electrospray ionization mass spectrometry. Three predominant immunoreactive proteins were found in the TES; all three had been previously described in the literature: Tc-CTL-1, Tc-TES-26, and Tc-MUC-3. We generated Escherichia coli expressed recombinant proteins for evaluation in Luminex based immunoassays. We were unable to produce a functional assay with the Tc-MUC-3 recombinant protein. Tc-CTL-1 and Tc-TES-26 were successfully coupled and tested using defined serum batteries. The use of both proteins together generated better results than if the proteins were used individually. The sensitivity and specificity of the assay for detecting visceral larval migrans using Tc-CTL-1 plus Tc-TES-26 was 99% and 94%, respectively; the sensitivity for detecting ocular larval migrans was 64%. The combined performance of the new assay was superior to the currently available EIA and could potentially be employed to replace current assays that rely on native TES-Ag.

Luminex and other multiplex high throughput technologies for the identification of, and host response to, environmental triggers of type 1 diabetes.

Science.gov (United States)

Purohit, Sharad; Sharma, Ashok; She, Jin-Xiong

2015-01-01

Complex interactions between a series of environmental factors and genes result in progression to clinical type 1 diabetes in genetically susceptible individuals. Despite several decades of research in the area, these interactions remain poorly understood. Several studies have yielded associations of certain foods, infections, and immunizations with the onset and progression of diabetes autoimmunity, but most findings are still inconclusive. Environmental triggers are difficult to identify mainly due to (i) large number and complex nature of environmental exposures, including bacteria, viruses, dietary factors, and environmental pollutants, (ii) reliance on low throughput technology, (iii) less efforts in quantifying host response, (iv) long silent period between the exposure and clinical onset of T1D which may lead to loss of the exposure fingerprints, and (v) limited sample sets. Recent development in multiplex technologies has enabled systematic evaluation of different classes of molecules or macroparticles in a high throughput manner. However, the use of multiplex assays in type 1 diabetes research is limited to cytokine assays. In this review, we will discuss the potential use of multiplex high throughput technologies in identification of environmental triggers and host response in type 1 diabetes.

Amniotic fluid protein profiles of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria.

Directory of Open Access Journals (Sweden)

Marian Kacerovsky

Full Text Available OBJECTIVE: This study aimed to evaluate the amniotic fluid protein profiles and the intensity of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria, using the multiplex xMAP technology. METHODS: A retrospective cohort study was undertaken in the Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Czech Republic. A total of 145 pregnant women with preterm prelabor rupture of membranes between gestational age 24+0 and 36+6 weeks were included in the study. Amniocenteses were performed. The presence of Ureaplasma spp. and other bacteria was evaluated using 16S rRNA gene sequencing. The levels of specific proteins were determined using multiplex xMAP technology. RESULTS: The presence of Ureaplasma spp. and other bacteria in the amniotic fluid was associated with increased levels of interleukin (IL-6, IL-8, IL-10, brain-derived neurotropic factor, granulocyte macrophage colony stimulating factor, monocyte chemotactic protein-1, macrophage inflammatory protein-1, and matrix metalloproteinasis-9. Ureaplasma spp. were also associated with increased levels of neurotropin-3 and triggering receptor expressed on myeloid cells-1. CONCLUSIONS: The presence of Ureaplasma spp. in the amniotic fluid is associated with a slightly different protein profile of inflammatory response, but the intensity of inflammatory response to Ureaplasma spp. is comparable with the inflammatory response to other bacteria.

Cytokine Correlations in Youth with Tic Disorders

Science.gov (United States)

Parker-Athill, E. Carla; Ehrhart, Jared; Tan, Jun

2015-01-01

Abstract Background: Studies have noted immunological disruptions in patients with tic disorders, including increased serum cytokine levels. This study aimed to determine whether or not cytokine levels could be correlated with tic symptom severity in patients with a diagnosed tic disorder. Methods: Twenty-one patients, ages 4–17 years (average 10.63±2.34 years, 13 males), with a clinical diagnosis of Tourette's syndrome (TS) or chronic tic disorder (CTD), were selected based on having clinic visits that coincided with a tic symptom exacerbation and a remission. Ratings of tic severity were assessed using the Yale Global Tic Severity Scale (YGTSS) and serum cytokine levels (interleukin [IL]-2, IL-4, IL-5, IL-10, IL-12p70, IL-13, interferon [IFN]-γ, tumor necrosis factor [TNF]-α, and granulocyte macrophage-colony stimulating factor [GM-CSF]) were measured using Luminex xMAP technology. Results: During tic symptom exacerbation, patients had higher median serum TNF-α levels (z=−1.962, p=0.05), particularly those on antipsychotics (U=9.00, p=0.033). Increased IL-13 was also associated with antipsychotic use during exacerbation (U=4.00, p=0.043) despite being negatively correlated to tic severity scores (ρ=−0.599, p=018), whereas increased IL-5 was associated with antibiotic use (U=6.5, p=0.035). During tic symptom remission, increased serum IL-4 levels were associated with antipsychotic (U=6.00, p=0.047) and antibiotic (U=1.00, p=0.016) use, whereas increased IL-12p70 (U=4.00, p=0.037) was associated with antibiotic use. Conclusions: These findings suggest a role for cytokine dysregulation in the pathogenesis of tic disorders. It also points toward the mechanistic involvement and potential diagnostic utility of cytokine monitoring, particularly TNF-α levels. Larger, systematic studies are necessary to further delineate the role of cytokines and medication influences on immunological profiling in tic disorders. PMID:25658821

Impact of follicular G-CSF quantification on subsequent embryo transfer decisions: a proof of concept study.

Science.gov (United States)

Lédée, N; Gridelet, V; Ravet, S; Jouan, C; Gaspard, O; Wenders, F; Thonon, F; Hincourt, N; Dubois, M; Foidart, J M; Munaut, C; Perrier d'Hauterive, S

2013-02-01

Previous experiments have shown that granulocyte colony-stimulating factor (G-CSF), quantified in the follicular fluid (FF) of individual oocytes, correlates with the potential for an ongoing pregnancy of the corresponding fertilized oocytes among selected transferred embryos. Here we present a proof of concept study aimed at evaluating the impact of including FF G-CSF quantification in the embryo transfer decisions. FF G-CSF was quantified with the Luminex XMap technology in 523 individual FF samples corresponding to 116 fresh transferred embryos, 275 frozen embryos and 131 destroyed embryos from 78 patients undergoing ICSI. Follicular G-CSF was highly predictive of subsequent implantation. The receiving operator characteristics curve methodology showed its higher discriminatory power to predict ongoing pregnancy in multivariate logistic regression analysis for FF G-CSF compared with embryo morphology [0.77 (0.69-0.83), P Embryos were classified by their FF G-CSF concentration: Class I over 30 pg/ml (a highest positive predictive value for implantation), Class II from 30 to 18.4 pg/ml and Class III Embryos derived from Class I follicles had a significantly higher implantation rate (IR) than those from Class II and III follicles (36 versus 16.6 and 6%, P Embryos derived from Class I follicles with an optimal morphology reached an IR of 54%. Frozen-thawed embryos transfer derived from Class I follicles had an IR of 37% significantly higher than those from Class II and III follicles, respectively, of 8 and 5% (P embryos but also 10% of the destroyed embryos were derived from G-CSF Class I follicles. Non-optimal embryos appear to have been transferred in 28% (22/78) of the women, and their pregnancy rate was significantly lower than that of women who received at least one optimal embryo (18 versus 36%, P = 0.04). Monitoring FF G-CSF for the selection of embryos with a better potential for pregnancy might improve the effectiveness of IVF by reducing the time and cost

Cytokine correlations in youth with tic disorders.

Science.gov (United States)

Parker-Athill, E Carla; Ehrhart, Jared; Tan, Jun; Murphy, Tanya K

2015-02-01

Studies have noted immunological disruptions in patients with tic disorders, including increased serum cytokine levels. This study aimed to determine whether or not cytokine levels could be correlated with tic symptom severity in patients with a diagnosed tic disorder. Twenty-one patients, ages 4-17 years (average 10.63±2.34 years, 13 males), with a clinical diagnosis of Tourette's syndrome (TS) or chronic tic disorder (CTD), were selected based on having clinic visits that coincided with a tic symptom exacerbation and a remission. Ratings of tic severity were assessed using the Yale Global Tic Severity Scale (YGTSS) and serum cytokine levels (interleukin [IL]-2, IL-4, IL-5, IL-10, IL-12p70, IL-13, interferon [IFN]-γ, tumor necrosis factor [TNF]-α, and granulocyte macrophage-colony stimulating factor [GM-CSF]) were measured using Luminex xMAP technology. During tic symptom exacerbation, patients had higher median serum TNF-α levels (z=-1.962, p=0.05), particularly those on antipsychotics (U=9.00, p=0.033). Increased IL-13 was also associated with antipsychotic use during exacerbation (U=4.00, p=0.043) despite being negatively correlated to tic severity scores (ρ=-0.599, p=018), whereas increased IL-5 was associated with antibiotic use (U=6.5, p=0.035). During tic symptom remission, increased serum IL-4 levels were associated with antipsychotic (U=6.00, p=0.047) and antibiotic (U=1.00, p=0.016) use, whereas increased IL-12p70 (U=4.00, p=0.037) was associated with antibiotic use. These findings suggest a role for cytokine dysregulation in the pathogenesis of tic disorders. It also points toward the mechanistic involvement and potential diagnostic utility of cytokine monitoring, particularly TNF-α levels. Larger, systematic studies are necessary to further delineate the role of cytokines and medication influences on immunological profiling in tic disorders.

LUMINEX®: a new technology for the simultaneous identification of five Entamoeba spp. commonly found in human stools.

Science.gov (United States)

Santos, Helena Lúcia Carneiro; Bandyopadhyay, Kakali; Bandea, Rebecca; Peralta, Regina Helena Saramago; Peralta, José Mauro; Da Silva, Alexandre Januário

2013-03-15

Six species of the genus Entamoeba, i.e., E. histolytica, E. dispar, E. moshkovskii, E. polecki, E. coli, and E. hartmanii can be found in human stools. Among these, only E. histolytica is considered to be pathogenic, causing intestinal and extra-intestinal disease, but it is morphologically identical to E. dispar and E. moshkovskii. In general, E. polecki, E. coli, and E. hartmanii can be differentiated morphologically from E. histolytica, but some of their diagnostic morphologic features may overlap creating issues for the differential diagnosis. Moreover, the previous inability to differentiate among Entamoeba species has limited epidemiologic information on E histolytica. The objective of this study was to develop a rapid, high-throughput screening method using Luminex technique for the simultaneous detection and differentiation of Entamoeba species. PCR amplification was performed with biotinylated Entamoeba sp 18S rRNA gene primers, designed to amplify a fragment ranging from 382 to 429 bp of the Entamoeba spp studied. Regions of this fragment that could differentiate among E. histolytica, E. moshkovskii, E. dispar, E. hartmanii and E. coli were selected to design hybridization probes to link to Luminex beads. The assay was standardized with cloned DNA samples of each species and evaluated with 24 DNA extracts from samples obtained from individuals diagnosed with these amebas in their stools. Using this approach we were able to correctly identify E. histoltyica, E. dispar, E hartmanni, E. coli and E. moshkovskii in all specimens studied. From twenty four samples tested by microscopy, PCR/DNA Sequencing and real-time PCR, 100% agreed with PCR-Luminex assay for identification of E. dispar, E. moshkovskii, E. hartmanni, E. histolytica, and E. coli. These results show that this method could be used in the diagnostic detection of Entamoeba spp in fecal samples. This diagnostic test was useful to clearly distinguish E histolytica from other species and also to

Development and validation of a multiplex add-on assay for sepsis biomarkers using xMAP technology

DEFF Research Database (Denmark)

Kofoed, Kristian; Schneider, Uffe Vest; Scheel, Troels

2006-01-01

BACKGROUND: Sepsis is a common and often fatal disease. Because sepsis can be caused by many different organisms, biomarkers that can aid in diagnosing sepsis and monitoring treatment efficacy are highly warranted. New sepsis markers may provide additional information to complement the currently...... triggering receptor expressed on myeloid cells-1 (sTREM-1), and macrophage migration inhibiting factor (MIF) was developed and validated in-house. This 3-plex assay was added to a commercially available interleukin-1beta (IL-1beta), IL-6, IL-8, granulocyte/macrophage colony-stimulating factor, and tumor...

Development and validation of a multiplex add-on assay for sepsis biomarkers using xMAP technology

DEFF Research Database (Denmark)

Kofoed, Kristian; Schneider, Uffe Vest; Scheel, Troels

2006-01-01

Sepsis is a common and often fatal disease. Because sepsis can be caused by many different organisms, biomarkers that can aid in diagnosing sepsis and monitoring treatment efficacy are highly warranted. New sepsis markers may provide additional information to complement the currently used markers....

Production of recombinant proteins GST L1, E6 and E7 tag HPV 16 ...

African Journals Online (AJOL)

STORAGESEVER

2009-02-04

Feb 4, 2009 ... targeting the viral oncoproteins E6 and E7 are markers for HPV-associated ... Luminex XYP plate handler, Luminex SD sheath fluid delivery system, a Pentium 4 .... expression mediated by a potato virus X derived vector of the E7 protein .... inflammation, and antioxidant nutrients – assessing their roles as.

Microarray Beads for Identifying Blood Group Single Nucleotide Polymorphisms

OpenAIRE

Drago, Francesca; Karpasitou, Katerina; Poli, Francesca

2009-01-01

We have developed a high-throughput system for single nucleotide polymorphism (SNP) genotyping of alleles of diverse blood group systems exploiting Luminex technology. The method uses specific oligonucleotide probes coupled to a specific array of fluorescent microspheres and is designed for typing Jka/Jkb, Fya/Fyb, S/s, K/k, Kpa/Kpb, Jsa/Jsb, Coa/Cob and Lua/Lub alleles. Briefly, two multiplex PCR reactions (PCR I and PCR II) according to the laboratory specific needs are set up. PCR I amplif...

Dual energy computed tomography for the head.

Science.gov (United States)

Naruto, Norihito; Itoh, Toshihide; Noguchi, Kyo

2018-02-01

Dual energy CT (DECT) is a promising technology that provides better diagnostic accuracy in several brain diseases. DECT can generate various types of CT images from a single acquisition data set at high kV and low kV based on material decomposition algorithms. The two-material decomposition algorithm can separate bone/calcification from iodine accurately. The three-material decomposition algorithm can generate a virtual non-contrast image, which helps to identify conditions such as brain hemorrhage. A virtual monochromatic image has the potential to eliminate metal artifacts by reducing beam-hardening effects. DECT also enables exploration of advanced imaging to make diagnosis easier. One such novel application of DECT is the X-Map, which helps to visualize ischemic stroke in the brain without using iodine contrast medium.

Antigen-induced cytokine and chemokine release test for tuberculosis infection using adsorption of stimulated whole blood on filter paper and multiplex analysis

DEFF Research Database (Denmark)

Skogstrand, Kristin; Thysen, Anna H; Jørgensen, Charlotte S

2012-01-01

the spotting technique can make it suitable for use in locations without facilities like a centrifuge and a refrigerator. Materials and methods: Samples from 22 individuals undergoing screening for MTI and 10 healthy controls were incubated, centrifuged and IFN-γ measured by Enzyme-linked immunosorbent assay...... (ELISA), as described in the kit insert. In parallel, activated blood was spotted on filter paper (Schleicher & Schuell) and dried. The dried blood spot samples were analysed for 21 inflammatory markers with an in-house assay based on Luminex technology. Results: Our multiplex measurements...

Development and validation of a multiplex add-on assay of biomarkers related to sepsis using xMAP technology

DEFF Research Database (Denmark)

Kofoed, Kristian; Vest Schneider, Uffe; Scheel, Troels

2006-01-01

BACKGROUND: Sepsis is a common and often fatal disease. Because sepsis can be caused by many different organisms, biomarkers that can aid in diagnosing sepsis and monitoring treatment efficacy are highly warranted. New sepsis markers may provide additional information to complement the currently...... triggering receptor expressed on myeloid cells-1 (sTREM-1), and macrophage migration inhibiting factor (MIF) was developed and validated in-house. This 3-plex assay was added to a commercially available interleukin-1beta (IL-1beta), IL-6, IL-8, granulocyte/macrophage colony-stimulating factor, and tumor...

Simultaneous measurement of 25 inflammatory markers and neurotrophins in neonatal dried blood spots by immunoassay with xMAP technology

DEFF Research Database (Denmark)

Skogstrand, Kristin; Thorsen, Poul; Nørgaard-Pedersen, Bent

2005-01-01

BACKGROUND: Inflammatory reactions and other events in early life may be part of the etiology of late-onset diseases, including cerebral palsy, autism, and type 1 diabetes. Most neonatal screening programs for congenital disorders are based on analysis of dried blood spot samples (DBSS), and stor...

Serotype determination of Salmonella by xTAG assay.

Science.gov (United States)

Zheng, Zhibei; Zheng, Wei; Wang, Haoqiu; Pan, Jincao; Pu, Xiaoying

2017-10-01

Currently, no protocols or commercial kits are available to determine the serotypes of Salmonella by using Luminex MAGPIX®. In this study, an xTAG assay for serotype determination of Salmonella suitable for Luminex MAGPIX® is described and 228 Salmonella isolates were serotype determined by this xTAG assay. The xTAG assay consists of two steps: 1) Multiplex PCR to amplify simultaneously O, H and Vi antigen genes of Salmonella, and 2) Magplex-TAG™ microsphere hybridization to identify accurately the specific PCR products of different antigens. Compared with the serotyping results of traditional serum agglutination test, the sensitivity and specificity of the xTAG assay were 95.1% and 100%, respectively. The agreement rate of these two assays was 95.2%. Compared with Luminex xMAP® Salmonella Serotyping Assay (SSA) kit, the advantages of this xTAG assay are: First, the magnetic beads make it applicable to both the Luminex®100/200™ and MAGPIX® systems. Second, only primers rather than both primers and probes are needed in the xTAG assay, and the process of coupling antigen-specific oligonucleotide probes to beads is circumvented, which make the xTAG assay convenient to be utilized by other laboratories. The xTAG assay may serve as a rapid alternative or complementary method for traditional Salmonella serotyping tests, especially for laboratories that utilize the MAGPIX® systems. Copyright © 2017 Elsevier B.V. All rights reserved.

Can rapid integrated polymerase chain reaction-based diagnostics for gastrointestinal pathogens improve routine hospital infection control practice? A diagnostic study.

Science.gov (United States)

Pankhurst, Louise; Macfarlane-Smith, Louissa; Buchanan, James; Anson, Luke; Davies, Kerrie; O'Connor, Lily; Ashwin, Helen; Pike, Graham; Dingle, Kate E; Peto, Timothy Ea; Wordsworth, Sarah; Walker, A Sarah; Wilcox, Mark H; Crook, Derrick W

2014-08-01

Every year approximately 5000-9000 patients are admitted to a hospital with diarrhoea, which in up to 90% of cases has a non-infectious cause. As a result, single rooms are 'blocked' by patients with non-infectious diarrhoea, while patients with infectious diarrhoea are still in open bays because of a lack of free side rooms. A rapid test for differentiating infectious from non-infectious diarrhoea could be very beneficial for patients. To evaluate MassCode multiplex polymerase chain reaction (PCR) for the simultaneous diagnosis of multiple enteropathogens directly from stool, in terms of sensitivity/specificity to detect four common important enteropathogens: Clostridium difficile, Campylobacter spp., Salmonella spp. and norovirus. A retrospective study of fixed numbers of samples positive for C. difficile (n = 200), Campylobacter spp. (n = 200), Salmonella spp. (n = 100) and norovirus (n = 200) plus samples negative for all these pathogens (n = 300). Samples were sourced from NHS microbiology laboratories in Oxford and Leeds where initial diagnostic testing was performed according to Public Health England methodology. Researchers carrying out MassCode assays were blind to this information. A questionnaire survey, examining current practice for infection control teams and microbiology laboratories managing infectious diarrhoea, was also carried out. MassCode assays were carried out at Oxford University Hospitals NHS Trust. Further multiplex assays, carried out using Luminex, were run on the same set of samples at Leeds Teaching Hospitals NHS Trust. The questionnaire was completed by various NHS trusts. Sensitivity and specificity to detect C. difficile, Campylobacter spp., Salmonella spp., and norovirus. Nucleic acids were extracted from 948 clinical samples using an optimised protocol (200 Campylobacter spp., 199 C. difficile, 60 S. enterica, 199 norovirus and 295 negative samples; some samples contained more than one pathogen). Using the Mass

Enhanced target-specific signal detection using an Escherichia coli lysate in multiplex microbead immunoassays with E. coli-derived recombinant antigens.

Science.gov (United States)

Crestani, Sandra; Leitolis, Amanda; Lima, Lucianna Freitas Oliveira; Krieger, Marco A; Foti, Leonardo

2016-08-01

Diverse techniques have been developed to analyze antibody-mediated responses to infections. However, the most common tests, i.e., enzyme-linked immunosorbent assays, require separate reactions for each antigen and consequently necessitate large sample volumes. Luminex technology allows the detection of multiple antibodies in a single experiment, but nonspecific binding can impair the results. Therefore, we examined the use of Escherichia coli lysates to reduce nonspecific binding and improve the results of liquid microarrays based on Luminex technology. Anti-bacteria antibodies were detected in human serum samples, as evidenced by high median fluorescence intensity (MFI) in assays performed with paramagnetic microspheres coupled with E. coli lysates. Moreover, the addition of an E. coli lysate as a blocker reduced the nonspecific binding of antigens produced by E. coli in a concentration-dependent manner. Tris-HCl reduced MFI values in negative samples, but did not affect MFI for positive samples. For microspheres coupled with different antigens, an E. coli lysate blocker significantly improved the fluorescence signals from positive samples. The addition of Tris-HCl and the E. coli lysate induced antigen-specific differences in MFI. This combination of the E. coli lysate blocker and Tris-HCl yielded a statistically significant improvement in MFI in the assays for Chagas disease and hepatitis C virus samples. However, for the Treponema pallidum p47 antigen improvement in MFI was only observed for the preparation with the E. coli blocker at a concentration of 3%. In conclusion, the addition of an E. coli lysate and Tris-HCl to the microarray assay reduced the nonspecific binding of human anti-bacteria antibodies and, therefore, increased the specific MFI. Copyright © 2016 Elsevier B.V. All rights reserved.

Genome-Wide Mutagenesis in Borrelia burgdorferi.

Science.gov (United States)

Lin, Tao; Gao, Lihui

2018-01-01

Signature-tagged mutagenesis (STM) is a functional genomics approach to identify bacterial virulence determinants and virulence factors by simultaneously screening multiple mutants in a single host animal, and has been utilized extensively for the study of bacterial pathogenesis, host-pathogen interactions, and spirochete and tick biology. The signature-tagged transposon mutagenesis has been developed to investigate virulence determinants and pathogenesis of Borrelia burgdorferi. Mutants in genes important in virulence are identified by negative selection in which the mutants fail to colonize or disseminate in the animal host and tick vector. STM procedure combined with Luminex Flex ® Map™ technology and next-generation sequencing (e.g., Tn-seq) are the powerful high-throughput tools for the determination of Borrelia burgdorferi virulence determinants. The assessment of multiple tissue sites and two DNA resources at two different time points using Luminex Flex ® Map™ technology provides a robust data set. B. burgdorferi transposon mutant screening indicates that a high proportion of genes are the novel virulence determinants that are required for mouse and tick infection. In this protocol, an effective signature-tagged Himar1-based transposon suicide vector was developed and used to generate a sequence-defined library of nearly 4800 mutants in the infectious B. burgdorferi B31 clone. In STM, signature-tagged suicide vectors are constructed by inserting unique DNA sequences (tags) into the transposable elements. The signature-tagged transposon mutants are generated when transposon suicide vectors are transformed into an infectious B. burgdorferi clone, and the transposable element is transposed into the 5'-TA-3' sequence in the B. burgdorferi genome with the signature tag. The transposon library is created and consists of many sub-libraries, each sub-library has several hundreds of mutants with same tags. A group of mice or ticks are infected with a mixed

Multiplex serology of paraneoplastic antineuronal antibodies.

Science.gov (United States)

Maat, Peter; Brouwer, Eric; Hulsenboom, Esther; VanDuijn, Martijn; Schreurs, Marco W J; Hooijkaas, Herbert; Smitt, Peter A E Sillevis

2013-05-31

Paraneoplastic neurological syndromes (PNS) are devastating neurological disorders secondary to cancer, associated with onconeural autoantibodies. Such antibodies are directed against neuronal antigens aberrantly expressed by the tumor. The detection of onconeural antibodies in a patient is extremely important in diagnosing a neurological syndrome as paraneoplastic (70% is not yet known to have cancer) and in directing the search for the underlying neoplasm. At present six onconeural antibodies are considered 'well characterized' and recognize the antigens HuD, CDR62 (Yo), amphiphysin, CRMP-5 (CV2), NOVA-1 (Ri), and Ma2. The gold standard of detection is the characteristic immunohistochemical staining pattern on brain tissue sections combined with confirmation by immunoblotting using recombinant purified proteins. Since all six onconeural antibodies are usually analyzed simultaneously and objective cut-off values for these analyses are warranted, we developed a multiplex assay based on Luminex technology. Reaction of serial dilutions of six onconeural standard sera with microsphere-bound antigens showed lower limits of detection than with Western blotting. Using the six standard sera at a dilution of 1:200, the average within-run coefficient of variation (CV) was 4% (range 1.9-7.3%). The average between-run within-day CV was 5.1% (range 2.9-6.7%) while the average between-day CV was 8.1% (range 2.8-11.6%). The shelf-life of the antigen coupled microspheres was at least two months. The sensitivity of the multiplex assay ranged from 83% (Ri) to 100% (Yo, amphiphysin, CV2) and the specificity from 96% (CV2) to 100% (Ri). In conclusion, Luminex-based multiplex serology is highly reproducible with high sensitivity and specificity for the detection of onconeural antibodies. Conventional immunoblotting for diagnosis of onconeural antibodies in the setting of a routine laboratory may be replaced by this novel, robust technology. Copyright © 2013 Elsevier B.V. All rights

Cytokine concentration in aqueous humor of eyes with diabetic macular edema.

Science.gov (United States)

Jonas, Jost B; Jonas, Rahul A; Neumaier, Michael; Findeisen, Peter

2012-01-01

To measure cytokine concentrations in aqueous humor of eyes with diffuse diabetic macular edema. The interventional clinical comparative study included a study group of 23 patients with diffuse diabetic macular edema and a control group of 22 patients undergoing cataract surgery. Cytokine concentrations were measured in aqueous humor samples using a Luminex xMAP suspension array technology. In the study group as compared with the control group, significantly higher concentrations were measured for epidermal growth factor (P < 0.001), human growth factor (P < 0.001), intercellular adhesion molecule-1 (ICAM-1; P < 0.001), interleukin (IL)-1a2 (P = 0.04), IL-6 (P = 0.001), IL-8 (P < 0.001), interferon gamma-induced protein (P = 0.004), monocyte chemoattractant protein-1 (P < 0.001), monokine induced by interferon gamma (P < 0.001), matrix metalloproteinase 1 (P = 0.02), matrix metalloproteinase 9 (P < 0.001), plasminogen activator inhibitor 1 (P < 0.001), placenta growth factor (P < 0.001), tissue growth factor beta (P = 0.003), vascular cell adhesion molecule (P < 0.001), and vascular endothelial growth factor (P < 0.001). Retinal macula thickness was significantly associated with the concentrations of the epidermal growth factor (P = 0.005; ρ = 0.45), ICAM-1 (P < 0.001; ρ = 0.65), IL-3 (P = 0.002; ρ = 0.48), IL-6 (P = 0.003; ρ = 0.47), IL-8 (P < 0.001; ρ = 0.71), monocyte chemoattractant protein-1 (P = 0.001; ρ = 0.53), monokine induced by interferon gamma (P < 0.001; ρ = 0.57), matrix metalloproteinase 9 (P < 0.001; ρ = 0.61), tissue growth factor beta (P = 0.01; ρ = 0.42), placenta growth factor (P = 0.004; ρ = 0.46), vascular cell adhesion molecule (P = 0.006; ρ = 0.44), and vascular endothelial growth factor (P = 0.01; ρ = 0.42). In multivariate analysis, macular thickness remained to be significantly associated with the concentration of ICAM-1 (P = 0.03; r = 0.30). Vascular endothelial growth factor concentrations were correlated with concentration

Microarray Beads for Identifying Blood Group Single Nucleotide Polymorphisms.

Science.gov (United States)

Drago, Francesca; Karpasitou, Katerina; Poli, Francesca

2009-01-01

We have developed a high-throughput system for single nucleotide polymorphism (SNP) genotyping of alleles of diverse blood group systems exploiting Luminex technology. The method uses specific oligonucleotide probes coupled to a specific array of fluorescent microspheres and is designed for typing Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, K/k, Kp(a)/Kp(b), Js(a)/Js(b), Co(a)/Co(b) and Lu(a)/Lu(b) alleles. Briefly, two multiplex PCR reactions (PCR I and PCR II) according to the laboratory specific needs are set up. PCR I amplifies the alleles tested routinely, namely Jk(a)/Jk(b), Fy(a)/Fy(b), S/s, and K/k. PCR II amplifies those alleles that are typed less frequently. Biotinylated PCR products are hybridized in a single multiplex assay with the corresponding probe mixture. After incubation with R-phycoerythrin-conjugated streptavidin, the emitted fluorescence is analyzed with Luminex 100. So far, we have typed more than 2,000 subjects, 493 of whom with multiplex assay, and there have been no discrepancies with the serology results other than null and/or weak phenotypes. The cost of consumables and reagents for typing a single biallelic pair per sample is less than EUR 3.-, not including DNA extraction costs. The capability to perform multiplexed reactions makes the method markedly suitable for mass screening of red blood cell alleles. This genotyping approach represents an important tool in transfusion medicine.

Construction of large signaling pathways using an adaptive perturbation approach with phosphoproteomic data.

Science.gov (United States)

Melas, Ioannis N; Mitsos, Alexander; Messinis, Dimitris E; Weiss, Thomas S; Rodriguez, Julio-Saez; Alexopoulos, Leonidas G

2012-04-01

Construction of large and cell-specific signaling pathways is essential to understand information processing under normal and pathological conditions. On this front, gene-based approaches offer the advantage of large pathway exploration whereas phosphoproteomic approaches offer a more reliable view of pathway activities but are applicable to small pathway sizes. In this paper, we demonstrate an experimentally adaptive approach to construct large signaling pathways from phosphoproteomic data within a 3-day time frame. Our approach--taking advantage of the fast turnaround time of the xMAP technology--is carried out in four steps: (i) screen optimal pathway inducers, (ii) select the responsive ones, (iii) combine them in a combinatorial fashion to construct a phosphoproteomic dataset, and (iv) optimize a reduced generic pathway via an Integer Linear Programming formulation. As a case study, we uncover novel players and their corresponding pathways in primary human hepatocytes by interrogating the signal transduction downstream of 81 receptors of interest and constructing a detailed model for the responsive part of the network comprising 177 species (of which 14 are measured) and 365 interactions.

Excel file of salivary antibody analysis for Boqueron Beach study, Puerto Rico for six waterborne pathogens.

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset is the raw Luminex antibody responses to six common waterborne pathogens reported in MFI (Median Fluorescence Intensity) units. This dataset is...

HLA antibodies and soluble CD30 are associated with poor renal graft outcome: updated results of a single-center cross-sectional study.

Science.gov (United States)

Langan, Leanne L; D'Orsogna, Lloyd; Park, Lawrence P; Hughes, Tiffany L; Irish, Ashley; Luxton, Grant; Witt, Campbell S; Christiansen, Frank T

2006-01-01

In a previous study, we have shown that HLA class II antibodies and a high soluble CD30 (sCD30) measured at least 1 year post-transplant predict subsequent graft failure. We have now updated the results of this same cohort of 208 patients 15 months later. HLA-specific antibodies (class I and class II) were detected by ELISA LAT-M and Luminex LabScreen assays. Data on graft outcome was collected with a median follow-up of 4.7 years. By Kaplan-Meier analysis, class II antibody was again associated with a poorer outcome, with an estimated 6-year graft survival of 67% and 71% when detected by ELISA and Luminex, respectively, compared with 92% for those without class II antibody (p soluble CD30 level of > or = 100 U/ml was also associated with a poorer estimated 6-year graft survival (p = 0.02). HLA antibodies and high sCD30 (> or = 100 U/ml) had an additive effect such that those with both high sCD30 and class II antibodies had a hazard ratio for subsequent graft failure of 18.1 (p = 0.0008) and 8.6 (p = 0.007) when detected by ELISA and Luminex, respectively. These data show that detection of HLA class II antibodies and serum sCD30 measured at least 1 year post-transplant continues to predict a subsequent outcome up to 6 years after the initial measurement; they also show that such measures provide important information that may allow for modification of ongoing therapy.

Single Nucleotide Polymorphisms in Common Bean: Their Discovery and Genotyping Using a Multiplex Detection System

Directory of Open Access Journals (Sweden)

E. Gaitán-Solís

2008-11-01

Full Text Available Single nucleotide polymorphism (SNP markers are by far the most common form of DNA polymorphism in a genome. The objectives of this study were to discover SNPs in common bean ( L. by comparing sequences from coding and noncoding regions obtained from the GenBank and genomic DNA and to compare sequencing results with those obtained using single base extension (SBE assays on the Luminex-100 system for use in high-throughput germplasm evaluation. We assessed the frequency of SNPs in 47 fragments of common bean DNA, using SBE as the evaluation methodology. We conducted a sequence analysis of 10 genotypes of cultivated and wild beans belonging to the Mesoamerican and Andean genetic pools of . For the 10 genotypes evaluated, a total of 20,964 bp of sequence were analyzed in each genotype and compared, resulting in the discovery of 239 SNPs and 133 InDels, giving an average SNP frequency of one per 88 bp and an InDel frequency of one per 157 bp. This is the equivalent of a nucleotide diversity (θ of 6.27 × 10. Comparisons with the SNP genotypes previously obtained by direct sequencing showed that the SBE assays on the Luminex-100 were accurate, with 2.5% being miscalled and 1% showing no signal. These results indicate that the Luminex-100 provides a high-throughput system that can be used to analyze SNPs in large samples of genotypes both for purposes of assessing diversity and also for mapping studies.

South African Medical Journal - Vol 102, No 1 (2012)

African Journals Online (AJOL)

Gadzikanani Monyatsi, Paul C Mullan, Benjamin R Phelps, Michael A Tolle, Edwin M Machine, Floriza F Gennari, Jenny Makosky, Gabriel M Anabwani ... Luminex-based virtual crossmatching for renal transplantation in South Africa · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT ...

Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination

NARCIS (Netherlands)

Scherpenisse, Mirte; Schepp, Rutger M.; Mollers, Madelief; Mooij, Sofie H.; Meijer, Chris J. L. M.; Berbers, Guy A. M.; van der Klis, Fiona R. M.

2013-01-01

We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed

Analysis of Serum Levels of Cytokines, Chemokines, Growth Factors, and Monoamine Neurotransmitters in Patients With Tick-borne Encephalitis: Identification of Novel Inflammatory Markers With Implications for Pathogenesis

Czech Academy of Sciences Publication Activity Database

Palus, Martin; Formanová, P.; Salát, J.; Žampachová, E.; Elsterová, Jana; Růžek, Daniel

2015-01-01

Roč. 87, č. 5 (2015), s. 885-892 ISSN 0146-6615 R&D Projects: GA ČR GAP502/11/2116 Institutional support: RVO:60077344 Keywords : tick-borne encephalitis virus * neuroinflammation * luminex * neuroinfection Subject RIV: EE - Microbiology, Virology Impact factor: 1.998, year: 2015

Photon technology. Hard photon technology; Photon technology. Hard photon gijutsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

Research results of hard photon technology have been summarized as a part of novel technology development highly utilizing the quantum nature of photon. Hard photon technology refers to photon beam technologies which use photon in the 0.1 to 200 nm wavelength region. Hard photon has not been used in industry due to the lack of suitable photon sources and optical devices. However, hard photon in this wavelength region is expected to bring about innovations in such areas as ultrafine processing and material synthesis due to its atom selective reaction, inner shell excitation reaction, and spatially high resolution. Then, technological themes and possibility have been surveyed. Although there are principle proposes and their verification of individual technologies for the technologies of hard photon generation, regulation and utilization, they are still far from the practical applications. For the photon source technology, the laser diode pumped driver laser technology, laser plasma photon source technology, synchrotron radiation photon source technology, and vacuum ultraviolet photon source technology are presented. For the optical device technology, the multi-layer film technology for beam mirrors and the non-spherical lens processing technology are introduced. Also are described the reduction lithography technology, hard photon excitation process, and methods of analysis and measurement. 430 refs., 165 figs., 23 tabs.

Detection of HLA Antibodies in Organ Transplant Recipients – Triumphs and Challenges of the Solid Phase Bead Assay

Science.gov (United States)

Tait, Brian D.

2016-01-01

This review outlines the development of human leukocyte antigen (HLA) antibody detection assays and their use in organ transplantation in both antibody screening and crossmatching. The development of sensitive solid phase assays such as the enzyme-linked immunosorbent assay technique, and in particular the bead-based technology has revolutionized this field over the last 10–15 years. This revolution however has created a new paradigm in clinical decision making with respect to the detection of low level pretransplant HLA sensitization and its clinical relevance. The relative sensitivities of the assays used are discussed and the relevance of conflicting inter-assay results. Each assay has its advantages and disadvantages and these are discussed. Over the last decade, the bead-based assay utilizing the Luminex® fluorocytometer instrument has become established as the “gold standard” for HLA antibody testing. However, there are still unresolved issues surrounding this technique, such as the presence of denatured HLA molecules on the beads which reveal cryptic epitopes and the issue of appropriate fluorescence cut off values for positivity. The assay has been modified to detect complement binding (CB) in addition to non-complement binding (NCB) HLA antibodies although the clinical relevance of the CB and NCB IgG isotypes is not fully resolved. The increase sensitivity of the Luminex® bead assay over the complement-dependent cytotoxicity crossmatch has permitted the concept of the “virtual crossmatch” whereby the crossmatch is predicted to a high degree of accuracy based on the HLA antibody specificities detected by the solid phase assay. Dialog between clinicians and laboratory staff on an individual patient basis is essential for correct clinical decision making based on HLA antibody results obtained by the various techniques. PMID:28018342

Development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species

NARCIS (Netherlands)

Bald-Blume, Niklas; Bergervoet, Jan H.W.; Maiss, Edgar

2017-01-01

A Luminex xTAG-based assay for plant-infecting tospoviruses was developed. The test enables the detection of tospoviruses in general and the differentiation of the four important member species of this genus: Tomato spotted wilt virus, Impatiens necrotic spot virus, the proposed ‘Capsicum chlorosis

Dataset of aqueous humor cytokine profile in HIV patients with Cytomegalovirus (CMV retinitis

Directory of Open Access Journals (Sweden)

Jayant Venkatramani Iyer

2016-09-01

Full Text Available The data shows the aqueous humor cytokine profiling results acquired in a small cohort of 17 HIV patients clinically diagnosed with Cytomegalovirus retinitis using the FlexMAP 3D (Luminex® platform using the Milliplex Human Cytokine® kit. Aqueous humor samples were collected from these patients at different time points (pre-treatment and at 4-weekly intervals through the 12-week course of intravitreal ganciclovir treatment and 41 cytokine levels were analyzed at each time point. CMV DNA viral load was assessed in 8 patients at different time points throughout the course of ganciclovir treatment. The data described herein is related to the research article entitled “Aqueous humor immune factors and cytomegalovirus (CMV levels in CMV retinitis through treatment - The CRIGSS study” (Iyer et al., 2016 [1]. Cytokine levels against the different time points which indicate the response to the given treatment and against the CMV viral load were analyzed. Keywords: Cytokines, CMV retinitis, Dataset, HIV, Luminex bead assay

Neonatal levels of cytokines and risk of autism spectrum disorders

DEFF Research Database (Denmark)

Abdallah, Morsi; Larsen, Nanna; Mortensen, Erik L

2012-01-01

The aim of the study was to analyze cytokine profiles in neonatal dried blood samples (n-DBSS) retrieved from The Danish Newborn Screening Biobank of children developing Autism Spectrum Disorders (ASD) later in life and controls. Samples of 359 ASD cases and 741 controls were analyzed using Luminex...

Photon technology. Laser process technology; Photon technology. Laser process gijutsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-03-01

For developing laser process technology by interaction between substance and photon, the present state, system, R and D issues and proposal of such technology were summarized. Development of the photon technology aims at the modification of bonding conditions of substances by quantum energy of photon, and the new process technology for generating ultra- high temperature and pressure fields by concentrating photon on a minute region. Photon technology contributes to not only the conventional mechanical and thermal forming and removal machining but also function added machining (photon machining) in quantum level and new machining technology ranging from macro- to micro-machining, creating a new industrial field. This technology extends various fields from the basis of physics and chemistry to new bonding technology. Development of a compact high-quality high-power high-efficiency photon source, and advanced photon transmission technology are necessary. The basic explication of an unsolved physicochemical phenomenon related to photon and substance, and development of related application technologies are essential. 328 refs., 147 figs., 13 tabs.

Effect of Sacral Neuromodulation on Outcome Measures and Urine Chemokines in Interstitial Cystitis/Painful Bladder Syndrome Patients.

Science.gov (United States)

Peters, Kenneth M; Jayabalan, Nirmal; Bui, Don; Killinger, Kim; Chancellor, Michael; Tyagi, Pradeep

2015-05-01

Sacral neuromodulation (SNM) may improve interstitial cystitis/painful bladder syndrome (IC/BPS) symptoms of urinary frequency, urgency and perhaps even pain, but objective measures of improvement are lacking. We evaluated the potential for urinary chemokines to serve as measures of treatment response over time to SNM. Women with IC/BPS undergoing SNM consented for this study. Three-day bladder/pain diaries were collected at baseline and validated Interstitial Cystitis Symptom Problem Index (ICSPI) scores and mid-stream urine specimens were collected at baseline and at 24 weeks after successful implant. Collected urine was screened for infection by dipstick and analyzed for chemokines by luminex xMAP analysis. At baseline (n = 16), urine levels of CXCL-1 positively correlated with pain score (r = 0.63, P = 0.009), urgency (r = 0.61, P = 0.01), ICSPI (r = 0.43, P = 0.09) and daily voids (r = 0.44, P = 0.08). ICSPI and pain scores also positively correlated with sIL-1ra (r = 0.50, P = 0.04) and monocyte chemotactic protein-1 (MCP-1) or CCL2 positively correlated with daily voids (r = 0.45, P = 0.07) only. At 24 weeks, the median ICSPI index fell from 28 to 15 (n = 7, P = 0.008). Urine levels of sIL-1ra (633.8 ± 188.2 vs. 149.9 ± 41.62 pg/mL) and MCP-1 (448.3 ± 11.6 vs. 176.9 ± 46.16 pg/mL) and CCL5 (20.78 ± 4.09 vs. 11.21 ± 4.12 pg/mL) were also significantly reduced at the follow-up relative to baseline values (P = 0.04). Multivariable analysis of data revealed that sIL-1ra and MCP-1 together explained the majority of variance in data. Levels of CXCL-1, CXCL-10, interleukin (IL)-8, vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF) were also reduced at 24 weeks, but differences were not significant. Concomitant decrease in urine levels of chemokines especially MCP-1 was associated with treatment response of SNM. These results

IP-10, MCP-1, MCP-2, MCP-3, and IL-1RA hold promise as biomarkers for infection with M. tuberculosis in a whole blood based T-cell assay

DEFF Research Database (Denmark)

Ruhwald, Morten; Bjerregaard-Andersen, Morten; Rabna, Paulo

2009-01-01

and mitogen in the Quantiferon In Tube test tubes. Levels of biomarkers were measured using Luminex and ELISA (IFN-gamma). RESULTS: We found all five new biomarkers were expressed in significantly higher concentrations compared to IFN-gamma. IP-10 and MCP-3 levels in the un-stimulated samples were higher...

Technological Criteria Technology-Environmental under a Systemic Approach: Chemistry Technology Transfer

Directory of Open Access Journals (Sweden)

Durán-García Martín Enrique

2014-07-01

Full Text Available Currently the transfer of chemical technology is a process that contributes to the technology policy of a country, an industry or an organization in general chemistry. This process requires the application of clear criteria for the proper development of the complex interrelations in the transfer of chemical technology. A group of criteria that are present, are those related to environmental technology which intrinsically define the technology and its impact to the environment. Therefore, the transfer of chemical technology requires technological-environmental criteria defining, in conjunction with other criteria, an adequate process for the selection, acquisition and incorporation of technology in a holistic perspective, so it provides feasible solutions the chemical industry in pursuit of their goals. Then the criterion becomes a benchmark for assessing an appropriate technology transfer process. We performed a theoretical analysis of the technological and environmental criteria, proposing thirty-six (36 technological-environmental criteria interrelated under a systemic approach in the process of transfer of chemical technology, focused on a methodological cycle first run, based primarily on the research-action method. Future research is expected to make a refinement of the criteria from the formulation and validation of metrics so that necessary adjustments are made to optimize the process of transfer of chemical technology.

Morgantown Energy Technology Center, technology summary

International Nuclear Information System (INIS)

1994-06-01

This document has been prepared by the DOE Environmental Management (EM) Office of Technology Development (OTD) to highlight its research, development, demonstration, testing, and evaluation activities funded through the Morgantown Energy Technology Center (METC). Technologies and processes described have the potential to enhance DOE's cleanup and waste management efforts, as well as improve US industry's competitiveness in global environmental markets. METC's R ampersand D programs are focused on commercialization of technologies that will be carried out in the private sector. META has solicited two PRDAs for EM. The first, in the area of groundwater and soil technologies, resulted in twenty-one contact awards to private sector and university technology developers. The second PRDA solicited novel decontamination and decommissioning technologies and resulted in eighteen contract awards. In addition to the PRDAs, METC solicited the first EM ROA in 1993. The ROA solicited research in a broad range of EM-related topics including in situ remediation, characterization, sensors, and monitoring technologies, efficient separation technologies, mixed waste treatment technologies, and robotics. This document describes these technology development activities

Technology round and management of technology

International Nuclear Information System (INIS)

Park, Yong Tae

1994-04-01

This book deals with beginning of technology round with background of it, change of scientific technique paradigm with economy, management and policy, change of international political environment globalization of technical and economic environment, formation of strategic alliance, intensifying regionalism, new GATT system, UR and technology round, new international technique regulation and technology round of OECD, feature and meaning of technology round, assignment and scientific technique of Korea, past and present of scientific technology in Korea, correspondence for technology round.

Technological Criteria Technology-Environmental under a Systemic Approach: Chemistry Technology Transfer

OpenAIRE

Durán-García Martín Enrique

2014-01-01

Currently the transfer of chemical technology is a process that contributes to the technology policy of a country, an industry or an organization in general chemistry. This process requires the application of clear criteria for the proper development of the complex interrelations in the transfer of chemical technology. A group of criteria that are present, are those related to environmental technology which intrinsically define the technology and its impact to the environment. Therefore, the ...

Development of Two FhSAP2 Recombinant–Based Assays for Immunodiagnosis of Human Chronic Fascioliasis

Science.gov (United States)

Shin, Sun Hee; Hsu, Angel; Chastain, Holly M.; Cruz, Lorna A.; Elder, Eric S.; Sapp, Sarah G. H.; McAuliffe, Isabel; Espino, Ana M.; Handali, Sukwan

2016-01-01

In the United States, infection with Fasciola hepatica has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as F. hepatica antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione S-transferase (GST)–FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect F. hepatica total IgG and IgG4 antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG4 WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG4, the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis. PMID:27549636

Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis.

Science.gov (United States)

Shin, Sun Hee; Hsu, Angel; Chastain, Holly M; Cruz, Lorna A; Elder, Eric S; Sapp, Sarah G H; McAuliffe, Isabel; Espino, Ana M; Handali, Sukwan

2016-10-05

In the United States, infection with Fasciola hepatica has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as F. hepatica antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione S-transferase (GST)-FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect F. hepatica total IgG and IgG 4 antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG 4 WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG 4 , the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis. © The American Society of Tropical Medicine and Hygiene.

Diversity of beta-papillomavirus at anogenital and oral anatomic sites of men: The HIM Study.

Science.gov (United States)

Nunes, Emily Montosa; Sudenga, Staci L; Gheit, Tarik; Tommasino, Massimo; Baggio, Maria Luiza; Ferreira, Silvaneide; Galan, Lenice; Silva, Roberto C; Pierce Campbell, Christine M; Lazcano-Ponce, Eduardo; Giuliano, Anna R; Villa, Luisa L; Sichero, Laura

2016-08-01

Our goal was to describe prevalence of β-HPVs at three anatomic sites among 717 men from Brazil, Mexico and US enrolled in the HPV Infection in Men (HIM) Study. β-HPVs were genotyped using Luminex technology. Overall, 77.7%, 54.3% and 29.3% men were positive for any β-HPV at the genitals, anal canal, and oral cavity, respectively. Men from US and Brazil were significantly less likely to have β-HPV at the anal canal than men from Mexico. Older men were more likely to have β-HPV at the anal canal compared to younger men. Prevalence of β-HPV at the oral cavity was significantly associated with country of origin and age. Current smokers were significantly less likely to have β-HPV in the oral cavity than men who never smoked. Lack of associations between β-HPV and sexual behaviors may suggest other routes of contact such as autoinoculation which need to be explored further. Copyright © 2016 Elsevier Inc. All rights reserved.

How Technology Teachers Understand Technological Knowledge

Science.gov (United States)

Norström, Per

2014-01-01

Swedish technology teachers' views of technological knowledge are examined through a written survey and a series of interviews. The study indicates that technology teachers' understandings of what constitutes technological knowledge and how it is justified vary considerably. The philosophical discussions on the topic are unknown to them. This lack…

Production of recombinant proteins GST L1, E6 and E7 tag HPV 16 ...

African Journals Online (AJOL)

In the present work recombinant proteins were produced for used in LUMINEX in order to undergo serological study of Tunisian female population. HPV types 16 L1, E6 and E7 sequences fused to their 3'-end to a sequence encoding the terminal undecapeptide of the SV40 large T-antigen (tag) were isolated from plasmids ...

Appropriate Technology as Indian Technology.

Science.gov (United States)

Barry, Tom

1979-01-01

Describes the mounting enthusiasm of Indian communities for appropriate technology as an inexpensive means of providing much needed energy and job opportunities. Describes the development of several appropriate technology projects, and the goals and activities of groups involved in utilizing low scale solar technology for economic development on…

Technology strategy for gas technologies; Technology Target Areas; TTA8 Gas Technology

Energy Technology Data Exchange (ETDEWEB)

2008-07-01

TTA8 - Gas technologies points out the various routes Norway can follow to capitalise on the vast resources of natural gas that will be produced in the years to come by developing a strong technology and competence platform. A broad view is taken for the value creation having as basis the continued gas export from NCS to Europe, but also a strong focus on development of gas resources in other parts of the world. The latter can also be seen as part of international positioning for upstream resources and does also include involvements in projects, and export of technology and products. The TTA has structured the analysis into 3 main areas: Gas transport and processing (pipeline, LNG, other); Gas conversion to fuels, chemicals and materials; CO{sub 2} management. In this report, for each of these areas, scenarios based on a gap analysis are presented. One of the key goals has been to identify pacing and emerging technologies for the next 20 years. Based on this, technologies have been mapped according to importance for future competitiveness and technology ambitions. This also includes primary funding responsibilities (public and/or industry). The road map below reflects the key issues in the proposed strategy. The base level of the figure explains areas that will have to be pursued to maintain Norway's role as a key gas and gas technology provider. The second layer represents near term options and possibilities with a reasonable risk profile that could further enhance the Norwegian position given the resources and drive to further develop this industry. As the top layer we have selected some of our 'dreams', what we may achieve if a progressive approach is followed with a strongly innovation based policy. It is acknowledged by the TTA that Norway cannot be a leading technology player in all aspects of the gas value chain. For some technologies we should be an active player and developer, whilst for other technologies we should become a competent buyer and user. This

Technology strategy for gas technologies; Technology Target Areas; TTA8 Gas Technology

Energy Technology Data Exchange (ETDEWEB)

2008-07-01

TTA8 - Gas technologies points out the various routes Norway can follow to capitalise on the vast resources of natural gas that will be produced in the years to come by developing a strong technology and competence platform. A broad view is taken for the value creation having as basis the continued gas export from NCS to Europe, but also a strong focus on development of gas resources in other parts of the world. The latter can also be seen as part of international positioning for upstream resources and does also include involvements in projects, and export of technology and products. The TTA has structured the analysis into 3 main areas: Gas transport and processing (pipeline, LNG, other); Gas conversion to fuels, chemicals and materials; CO{sub 2} management. In this report, for each of these areas, scenarios based on a gap analysis are presented. One of the key goals has been to identify pacing and emerging technologies for the next 20 years. Based on this, technologies have been mapped according to importance for future competitiveness and technology ambitions. This also includes primary funding responsibilities (public and/or industry). The road map below reflects the key issues in the proposed strategy. The base level of the figure explains areas that will have to be pursued to maintain Norway's role as a key gas and gas technology provider. The second layer represents near term options and possibilities with a reasonable risk profile that could further enhance the Norwegian position given the resources and drive to further develop this industry. As the top layer we have selected some of our 'dreams', what we may achieve if a progressive approach is followed with a strongly innovation based policy. It is acknowledged by the TTA that Norway cannot be a leading technology player in all aspects of the gas value chain. For some technologies we should be an active player and developer, whilst for other technologies we should become a competent buyer

Histocompatibility Testing for Organ Transplantation Purposes in Albania: A Single Center Experience

Directory of Open Access Journals (Sweden)

Erkena Shyti

2014-06-01

Full Text Available Background: Histocompatibility testing (HT which includes donor-recipient human leukocyte antigen (HLA matching, cross-match testing (XMT and anti-HLA antibody searching are crucial examinations in solid organ transplantation aiming to avoid the hyperacute graft rejection and also to predict the immunological outcome of the graft. Aims: The aim of this study was to analyse the tissue typing data collected at the Laboratory of Immunology and Histocompatibility of the University Hospital Center of Tirana, Albania, in order to define those actions that should be taken for improvements in the situation of kidney transplantation in Albania. Design: Descriptive study. Methods: The donor/recipient cross-match testing was performed through a standard complement-dependent cytotoxicity (CDC assay using separated donor T and B cells that were tested in parallel with the recipient serum sample. All recipient sera were screened for anti-Class I and anti-Class II HLA antibodies using a bead based Luminex anti-HLA antibody screening test. In the case of detected positivity, an allele-specific anti-HLA antibody determination was conducted with the respective Luminex anti-Class I and Class II HLA antibody determination kits. Results: A total of 174 recipients and 202 donors were typed for the purpose of living donor kidney transplantation at our laboratory between January 2006 and December 2012. The mean age and female gender proportion of patients were 34.9 years and 34.5%, respectively, and 48.0 years and 65.3% for the donors, respectively. Here, 25.9% of the patients reported a positive complement-dependent cytotoxicity cross-match test and/or a positive anti-HLA antibody testing result. Eighteen patients that were negative for the complement-dependent cytotoxicity cross-match test were positive for anti-HLA antibodies. Conclusion: The predominant causes of end-stage renal disease (ESRD in our patient population are chronic pyelonephritis and

Living technology: exploiting life's principles in technology.

Science.gov (United States)

Bedau, Mark A; McCaskill, John S; Packard, Norman H; Rasmussen, Steen

2010-01-01

The concept of living technology-that is, technology that is based on the powerful core features of life-is explained and illustrated with examples from artificial life software, reconfigurable and evolvable hardware, autonomously self-reproducing robots, chemical protocells, and hybrid electronic-chemical systems. We define primary (secondary) living technology according as key material components and core systems are not (are) derived from living organisms. Primary living technology is currently emerging, distinctive, and potentially powerful, motivating this review. We trace living technology's connections with artificial life (soft, hard, and wet), synthetic biology (top-down and bottom-up), and the convergence of nano-, bio-, information, and cognitive (NBIC) technologies. We end with a brief look at the social and ethical questions generated by the prospect of living technology.

Technology Transfer: Marketing Tomorrow's Technology

Science.gov (United States)

Tcheng, Erene

1995-01-01

The globalization of the economy and the end of the Cold War have triggered many changes in the traditional practices of U.S. industry. To effectively apply the resources available to the United States, the federal government has firmly advocated a policy of technology transfer between private industry and government labs, in this case the National Aeronautics and Space Administration (NASA). NASA Administrator Daniel Goldin is a strong proponent of this policy and has organized technology transfer or commercialization programs at each of the NASA field centers. Here at Langley Research Center, the Technology Applications Group (TAG) is responsible for facilitating the transfer of Langley developed research and technology to U.S. industry. Entering the program, I had many objectives for my summer research with TAG. Certainly, I wanted to gain a more thorough understanding of the concept of technology transfer and Langley's implementation of a system to promote it to both the Langley community and the community at large. Also, I hoped to become more familiar with Langley's research capabilities and technology inventory available to the public. More specifically, I wanted to learn about the technology transfer process at Langley. Because my mentor is a member of Materials and Manufacturing marketing sector of the Technology Transfer Team, another overriding objective for my research was to take advantage of his work and experience in materials research to learn about the Advanced Materials Research agency wide and help market these developments to private industry. Through the various projects I have been assigned to work on in TAG, I have successfully satisfied the majority of these objectives. Work on the Problem Statement Process for TAG as well as the development of the Advanced Materials Research Brochure have provided me with the opportunity to learn about the technology transfer process from the outside looking in and the inside looking out. Because TAG covers

Applications of aerospace technology in industry. A technology transfer profile: Food technology

Science.gov (United States)

Murray, D. M.

1971-01-01

Food processing and preservation technologies are reviewed, expected technological advances are considered including processing and market factors. NASA contributions to food technology and nutrition are presented with examples of transfer from NASA to industry.

Thermally activated technologies: Technology Roadmap

Energy Technology Data Exchange (ETDEWEB)

None, None

2003-05-01

The purpose of this Technology Roadmap is to outline a set of actions for government and industry to develop thermally activated technologies for converting America’s wasted heat resources into a reservoir of pollution-free energy for electric power, heating, cooling, refrigeration, and humidity control. Fuel flexibility is important. The actions also cover thermally activated technologies that use fossil fuels, biomass, and ultimately hydrogen, along with waste heat.

The United States Department of Energy Office of Industrial Technology`s Technology Benefits Recording System

Energy Technology Data Exchange (ETDEWEB)

Hughes, K.R.; Moore, N.L.

1994-09-01

The U.S. Department of Energy (DOE) Office of Industrial Technology`s (OIT`s) Technology Benefits Recording System (TBRS) was developed by Pacific Northwest Laboratory (PNL). The TBRS is used to organize and maintain records of the benefits accrued from the use of technologies developed with the assistance of OIT. OIT has had a sustained emphasis on technology deployment. While individual program managers have specific technology deployment goals for each of their ongoing programs, the Office has also established a separate Technology Deployment Division whose mission is to assist program managers and research and development partners commercialize technologies. As part of this effort, the Technology Deployment Division developed an energy-tracking task which has been performed by PNL since 1977. The goal of the energy-tracking task is to accurately assess the energy savings impact of OIT-developed technologies. In previous years, information on OIT-sponsored technologies existed in a variety of forms--first as a hardcopy, then electronically in several spreadsheet formats that existed in multiple software programs. The TBRS was created in 1993 for OIT and was based on information collected in all previous years from numerous industrial contacts, vendors, and plants that have installed OIT-sponsored technologies. The TBRS contains information on technologies commercialized between 1977 and the present, as well as information on emerging technologies in the late development/early commercialization stage of the technology life cycle. For each technology, details on the number of units sold and the energy saved are available on a year-by-year basis. Information regarding environmental benefits, productivity and competitiveness benefits, or impact that the technology may have had on employment is also available.

Space Transportation Technology Workshop: Propulsion Research and Technology

Science.gov (United States)

2000-01-01

This viewgraph presentation gives an overview of the Space Transportation Technology Workshop topics, including Propulsion Research and Technology (PR&T) project level organization, FY 2001 - 2006 project roadmap, points of contact, foundation technologies, auxiliary propulsion technology, PR&T Low Cost Turbo Rocket, and PR&T advanced reusable technologies RBCC test bed.

USE OF MULTIPARAMETER ANALYSIS OF LABORATORY BIOMARKERS TO ASSESS RHEUMATOID ARTHRITIS ACTIVITY

Directory of Open Access Journals (Sweden)

A. A. Novikov

2015-01-01

Full Text Available The key component in the management of patients with rheumatoid arthritis (RA is regular control of RA activity. The quantitative assessment of a patient’s status allows the development of standardized indications for anti-rheumatic therapy.Objective: to identify the laboratory biomarkers able to reflect RA activity.Subjects and methods. Fifty-eight patients with RA and 30 age- and sex-matched healthy donors were examined. The patients were divided into high/moderate and mild disease activity groups according to DAS28. The serum concentrations of 30 biomarkers were measured using immunonephelometric assay, enzyme immunoassay, and xMAP technology.Results and discussion. Multivariate analysis could identify the factors mostly related to high/moderate RA activity according to DAS28, such as fibroblast growth factor-2, monocyte chemoattractant protein-1, interleukins (IL 1α, 6, and 15, and tumor necrosis factor-α and could create a prognostic model for RA activity assessment. ROC analysis has shown that this model has excellent diagnostic efficiency in differentiating high/moderate versus low RA activity.Conclusion. To create a subjective assessment-independent immunological multiparameter index of greater diagnostic accuracy than the laboratory parameters routinely used in clinical practice may be a qualitatively new step in assessing and monitoring RA activity.

Emerging environmental technologies and environmental technology policy

Science.gov (United States)

Clarke, Leon Edward

This dissertation explores the role and design of environmental technology policy when environmental innovation is embodied in emerging environmental technologies such as photovoltaic cells or fuel cells. The dissertation consists of three individual studies, all of which use a simplified, general model industry between an emerging environmental technology and an entrenched, more-polluting technology. It clarifies the situations in which environmental technology policy can achieve high welfare and those in which it cannot; and it separates the possible situations an emerging environmental technology might face into four scenarios, each with its own technology policy recommendations. The second study attempts to clarify which of two factors is having a larger limiting effect on private investment in photovoltaics: the failure to internalize the environmental costs of fossil fuel electricity generation or a broad set of innovation market failures that apply to innovation irrespective of environmental concerns. The study indicates that innovation market failures are probably having a significantly larger impact than incomplete internalization. The third study explores the effectiveness of adoption subsidies at encouraging private-sector innovation. The conclusion is that adoption subsidies probably have only a limited effect on long-term, private-sector research. Two important general conclusions of the dissertation are (1) that optimal technology policy should begin with technology-push measures and end with demand-pull measures; and (2) that the technological response to internalization instruments, such as emissions taxes, may be highly nonlinear.

Nano technology

International Nuclear Information System (INIS)

Lee, In Sik

2002-03-01

This book is introduction of nano technology, which describes what nano technology is, alpha and omega of nano technology, the future of Korean nano technology and human being's future and nano technology. The contents of this book are nano period is coming, a engine of creation, what is molecular engineering, a huge nano technology, technique on making small things, nano materials with exorbitant possibility, the key of nano world the most desirable nano technology in bio industry, nano development plan of government, the direction of development for nano technology and children of heart.

Mediated Effects of Technology Competencies and Experiences on Relations among Attitudes Towards Technology Use, Technology Ownership, and Self Efficacy about Technological Pedagogical Content Knowledge

Science.gov (United States)

Yerdelen-Damar, Sevda; Boz, Yezdan; Aydın-Günbatar, Sevgi

2017-08-01

This study examined the relations of preservice science teachers' attitudes towards technology use, technology ownership, technology competencies, and experiences to their self-efficacy beliefs about technological pedagogical content knowledge (TPACK). The present study also investigated interrelations among preservice teachers' attitudes towards technology use, technology ownership, technology competencies, and experiences. The participants of study were 665 elementary preservice science teachers (467 females, 198 males) from 7 colleges in Turkey. The proposed model based on educational technology literature was tested using structural equation modeling. The model testing results revealed that preservice teachers' technology competencies and experiences mediated the relation of technology ownership to their TPACK self efficacy beliefs. The direct relation of their possession of technology to their TPACK self efficacy beliefs was insignificant while the indirect relation through their technology competencies and experiences was significant. The results also indicated there were significant direct effects of preservice teachers' attitudes towards technology use, technology competencies, and experiences on their TPACK self efficacy beliefs.

Photon technology. Laser processing technology; Photon technology. Laser process gijutsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

Survey has been conducted to develop laser processing technology utilizing the interaction between substance and photon. This is a part of the leading research on photon technology development. The photon technology development is aimed at novel technology development highly utilizing the quantum nature of photons. In the field of laser processing, high quality photons are used as tools, special functions of atoms and molecules will be discovered, and processing for functional fabrication (photon machining) will be established. A role of laser processing in industries has become significant, which is currently spreading not only into cutting and welding of materials and scalpels but also into such a special field as ultrafine processing of materials. The spreading is sometimes obstructed due to the difficulty of procurement of suitable machines and materials, and the increase of cost. The purpose of this study is to develop the optimal laser technology, to elucidate the interaction between substance and photon, and to develop the laser system and the transmission and regulation systems which realize the optimal conditions. 387 refs., 115 figs., 25 tabs.

Photon technology. Hard photon technology; Photon technology. Hard photon gijutsu

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-03-01

For the application of photon to industrial technologies, in particular, a hard photon technology was surveyed which uses photon beams of 0.1-200nm in wavelength. Its features such as selective atom reaction, dense inner shell excitation and spacial high resolution by quantum energy are expected to provide innovative techniques for various field such as fine machining, material synthesis and advanced inspection technology. This wavelength region has been hardly utilized for industrial fields because of poor development of suitable photon sources and optical devices. The developmental meaning, usable time and issue of a hard photon reduction lithography were surveyed as lithography in ultra-fine region below 0.1{mu}m. On hard photon analysis/evaluation technology, the industrial use of analysis, measurement and evaluation technologies by micro-beam was viewed, and optimum photon sources and optical systems were surveyed. Prediction of surface and surface layer modification by inner shell excitation, the future trend of this process and development of a vacuum ultraviolet light source were also surveyed. 383 refs., 153 figs., 17 tabs.

Environmental technology foresight : New horizons for technology management

NARCIS (Netherlands)

Den Hond, Frank; Groenewegen, Peter

1996-01-01

Decision-making in corporate technology management and government technology policy is increasingly influenced by the environmental impact of technologies. Technology foresight (TF) and environmental impact assessment (EIA) are analyzed with regard to the roles they can play in developing long-term

Technology certification and technology acceptance: Promoting interstate cooperation and market development for innovative technologies

International Nuclear Information System (INIS)

Brockbank, B.R.

1995-03-01

In the past two years, public and private efforts to promote development and deployment of innovative environmental technologies have shifted from the analysis of barriers to the implementation of a variety of initiatives aimed at surmounting those barriers. Particular attention has been directed at (1) streamlining fragmented technology acceptance processes within and among the states, and (2) alleviating disincentives, created by inadequate or unverified technology cost and performance data, for users and regulators to choose innovative technologies. Market fragmentation currently imposes significant cost burdens on technology developers and inhibits the investment of private capital in environmental technology companies. Among the responses to these problems are state and federal technology certification/validation programs, efforts to standardize cost/performance data reporting, and initiatives aimed at promoting interstate cooperation in technology testing and evaluation. This paper reviews the current status of these initiatives, identifies critical challenges to their success, and recommends strategies for addressing those challenges

Technology and ecological economics. Promethean technology, Pandorian potential

Energy Technology Data Exchange (ETDEWEB)

Small, Bruce [AgResearch Ltd., Private Bag 3123, Hamilton (New Zealand); Jollands, Nigel [New Zealand Centre for Ecological Economics, Massey University and Landcare Research Ltd, Private Bag 11052, Palmerston North (New Zealand)

2006-03-15

In considering social, economic and ecological impacts of new technologies it is essential to start from an understanding of human nature. This paper explores this issue drawing out some implications for ecological and neoclassical economics. The paper presents two key arguments. First, we argue that there is a growing tension between our evolved human nature and social structures and our emerging technological prowess. Modern technologies give us increasing power to manipulate the very axes of nature: space, time, energy, matter, and life. Technologies are now so powerful they give us abilities our ancestors would consider godlike. The question is posed: Are humans ready to wield the power of the gods? We have the knowledge, but do we have the wisdom? The myth of Prometheus and Pandora is considered as a metaphor for the interaction between technology, nature and universal aspects of human nature developed over eons of evolution. Second, we argue that even a 'technologically optimistic' scenario (employed by some economists) may not actually deliver Utopian outcomes. With technological advancement and diffusion there is a 'technological trickle down effect' whereby potent technologies, once available only to governments and powerful elites, become available to greater numbers of groups and individuals. The more accessible a technology, the more likely its social and ecological impacts will be shaped by the full range and extremes of human nature. These issues have implications for the development and regulation of Promethean technologies such as nuclear energy, genetic engineering and nanotechnology; technologies with unprecedented power and reach through nature. Development and diffusion of such technologies may also have implications for the ethics of the social structure of society. (author)

Science-Technology-Society or Technology-Society-Science? Insights from an Ancient Technology

Science.gov (United States)

Lee, Yeung Chung

2010-01-01

Current approaches to science-technology-society (STS) education focus primarily on the controversial socio-scientific issues that arise from the application of science in modern technology. This paper argues for an interdisciplinary approach to STS education that embraces science, technology, history, and social and cultural studies. By employing…

Technological economics: innovation, project management, and technology transfer

Energy Technology Data Exchange (ETDEWEB)

Bradbury, F R

1981-06-01

The relationship between economics and technology, as well as their interaction in production, productivity, project management, and in technology transfer processes are reviewed. Over the last two decades there has been an increasing interest by economists in the technologist's view of technical change and its mechanisms. The author looks at the zone between technology and economics, the technological economics, and discusses the theory of innovation recently sketched out by Nelson and Winter. The relevance to project management and technology transfer of contemporary writing by economists leads to the view that there are welcome signs of a convergence of the conceptual models now emerging and the practical problems of technology management and movement. Economists now seem more willing to come to terms with technology than technologists with economics. The economic significance of the multitudes of technically unglamorous activities in development work is seriously neglected as a result of over-emphasis on the spectacular technological break. If economic elegance were to be admitted to the criteria of success, one might get a significant improvement in the engineering of technological change. 29 references, 4 figure.

Spatial Dynamics of Technological Evolution: Technological Relatedness as Driver for Radical Emerging Technologies

DEFF Research Database (Denmark)

Tanner, Anne Nygaard

and applied knowledge in firms, universities, research institutes etc., and 2) the existence of risk-taking actors who are willing and capable of implementing and exploiting radical technologies. This paper focuses on the former. Where evolutionary economics have been occupied by accumulation of knowledge......Despite the consent of the fundamental role technological change plays for economic growth, it seems that little attention has been paid to how new technologies come into being. In particular, an understanding of the spatial and dynamic processes driving the emergence of radical technology...... is lacking. This paper seeks to fill out this research gap by bridging the school of evolutionary economics and the school of economic geography. Following Dosi (1988) two factors are in particular important for technological change in emerging technological paradigms: 1) accumulation of scientific...

Focus on Technologies: Worry or Technology?

Directory of Open Access Journals (Sweden)

Saulius Kanišauskas

2015-10-01

Full Text Available The paper analyses different attitudes towards technologies in contemporary philosophical discourses. It points out that classical notion of technology formulated by Martin Heidegger seems to be more and more often questioned and even forgotten. As a result, it is being replaced by the theory of determinism, according to which the change of technologies determines the changes in social systems, the human being including. This happens this way and not vice versa. Nowadays technē, or “technika” (in English: technology is mostly understood in the instrumental meaning or in the meaning of power. It is considered to be a powerful means, tool or mechanism to influence, change, control and manipulate human consciousness and human feelings. Despite the fact that technologies have already been tamed, the problem of huge responsibility for using and developing them arises. It is questioned whether the increasing society’s attention to modern technologies is not a particular “technology” of the postmodern capitalism to manipulate social consciousness. In parallel with “yes” answer to this question, Albert Borgmann’s idea that the causes of technological development have an ontological dimension, i.e. the causes are rooted in the nature of human beings themselves and their desire to adore own creativity, is discussed. Thus, it becomes necessary to probe deeper into the nature of creativity.

The technology vicinity: a location based view on technology

NARCIS (Netherlands)

Steenhuis, H.J.; de Bruijn, E.J.; Kocaoglu, D.F.; Anderson, T.R.

2001-01-01

The issue of technology transfer has been viewed from many different perspectives. In this case the focus is on the process of (production) technology transfer. One of the difficulties in studying international technology transfer is the definition of technology. The many technology definitions that

Art technologies as possible propulsars in technological innovation

DEFF Research Database (Denmark)

Jørgensen, Lars Botin

2003-01-01

kind. This discussion, of general character, constitutes the initial part of this paper. Then it briefly looks into the final terms of the papertitle and try to relate to the diffuse and discussed technological innovation. Both aspects – art and technology and technological innovation – will be dealt...... with from a historical/hermeneutic and social constructivist point of view, as the paper moves from a general principal level to a more specific, exemplary level, where three different art technologies are presented as possible propulsars in technological innovation.......Art and technology has, as the German philosopher Martin Heidegger points out, the same etymological root. A thorough investigation of the relationship between art and technology will show how this same root has manifested itself in different times and spaces bringing results of the most variegated...

Philosophy of Technology Assumptions in Educational Technology Leadership

Science.gov (United States)

Webster, Mark David

2017-01-01

A qualitative study using grounded theory methods was conducted to (a) examine what philosophy of technology assumptions are present in the thinking of K-12 technology leaders, (b) investigate how the assumptions may influence technology decision making, and (c) explore whether technological determinist assumptions are present. Subjects involved…

Live Webcasts from CERN and ESO for European Science and Technology Week

Science.gov (United States)

2002-10-01

Visit http://www.cern.ch/sci-tech on 7 - 8 November to find out what modern Europeans can't live without. Seven of Europe's leading Research Organizations [1] are presenting three live Webcasts from CERN in a joint outreach programme for the European Science and Technology Week . The aim of Sci-Tech... couldn't be without it! is to show how today's society couldn't be without cutting-edge scientific research. See also ESO Press Release 05/02. Northern Europeans can't imagine their households without ovens, whereas Southern Europeans identify the refrigerator as the most essential household appliance. In the area of communications, cars and motorbikes are clearly the technologies of choice in Italy, but are regarded as less important in countries like Norway and Germany. For entertainment, the personal computer is a clear winner as the device is considered most essential by all Europeans, followed by the TV and the Internet. This hit parade of technological marvels is the result of a phone and online survey conducted by the Sci-Tech... couldn't be without it! team for this year's European Science and Technology Week on 4-10 November. The technologies Europeans could not be without, form the starting point of three entertaining and informative Webcast shows in Italian (Thursday 7 November at 10:00 CET), French (Thursday 7 November at 15:00 CET) and English (Friday 8 November at 15:00 CET), broadcast live on the Internet from a studio at CERN. During these Webcasts scientists from the seven research Organizations and their industrial partners Sun Microsystems, Siemens, L'Oreal and Luminex will engage - from the CERN studio or from remote locations through teleconference links - an audience of Internauts all over the world. The public will be taken inside their most popular gadgets to discover the science that made them possible and how vital fundamental research has been in the creation of modern technology. Fundamental science will be brought as close as possible to

Relations between the technological standards and technological appropriation

Directory of Open Access Journals (Sweden)

Carlos Alberto PRADO GUERRERO

2010-06-01

Full Text Available The objective of this study is to analyze the educational practices of using Blackboard in blended learning environments with students of higher education to understand the relationship between technological appropriation and standards of educational technology. To achieve that goal, the following research question was raised: ¿To what extent are the standards of education technology with the appropriation of technology in blended learning environments in higher educa­tion related? The contextual framework of this work includes the following topics: the institution, teaching, teachers and students. The design methodology that was used is of a correlation type. Correlations were carried out to determine the frequency and level in the technological standards as well as the appropriation of technology. In the comparison of the results obtained by the students, the teachers and the platform; we found that students in the school study showed a high degree of technology ownership and this was the same for the performance shown on the technological standards. It was established that teachers play a key role in developing the techno­logical appropriation of students and performance in technology standards.

Emerging Technological Risk Underpinning the Risk of Technology Innovation

CERN Document Server

Anderson, Stuart

2012-01-01

Classes of socio-technical hazards allow a characterization of the risk in technology innovation and clarify the mechanisms underpinning emergent technological risk. Emerging Technological Risk provides an interdisciplinary account of risk in socio-technical systems including hazards which highlight: ·         How technological risk crosses organizational boundaries, ·         How technological trajectories and evolution develop from resolving tensions emerging between social aspects of organisations and technologies and ·         How social behaviour shapes, and is shaped by, technology. Addressing an audience from a range of academic and professional backgrounds, Emerging Technological Risk is a key source for those who wish to benefit from a detail and methodical exposure to multiple perspectives on technological risk. By providing a synthesis of recent work on risk that captures the complex mechanisms that characterize the emergence of risk in technology innovation, Emerging Tec...

Hydrogen technologies and the technology learning curve

International Nuclear Information System (INIS)

Rogner, H.-H.

1998-01-01

On their bumpy road to commercialization, hydrogen production, delivery and conversion technologies not only require dedicated research, development and demonstration efforts, but also protected niche markets and early adopters. While niche markets utilize the unique technological properties of hydrogen, adopters exhibit a willingness to pay a premium for hydrogen fueled energy services. The concept of the technology learning curve is applied to estimate the capital requirements associated with the commercialization process of several hydrogen technologies. (author)

The technology vicinity: a location based view on technology

OpenAIRE

Steenhuis, H.J.; de Bruijn, E.J.; Kocaoglu, D.F.; Anderson, T.R.

2001-01-01

The issue of technology transfer has been viewed from many different perspectives. In this case the focus is on the process of (production) technology transfer. One of the difficulties in studying international technology transfer is the definition of technology. The many technology definitions that exist are either too ‘loosely’ formulated or they require thorough expert knowledge. This results in difficulties with measuring technology and comparing different studies meaningfully. This study...

Constructive Technology Assessmentand Technology Dynamics. The Case of Clean Technologies

NARCIS (Netherlands)

Schot, Johan

1992-01-01

A synthesis of neo-Schumpeterian evolutionary, sociological, and historical coevolution ary models could be used for constructive technology assessment, aimed at the active management of the process of technological change. This article proposes a synthetic quasi-evolutionary model, in which

Technology assessment of thermal treatment technologies using ORWARE

International Nuclear Information System (INIS)

Assefa, G.; Eriksson, O.; Frostell, B.

2005-01-01

A technology assessment of thermal treatment technologies for wastes was performed in the form of scenarios of chains of technologies. The Swedish assessment tool, ORWARE, was used for the assessment. The scenarios of chains of thermal technologies assessed were gasification with catalytic combustion, gasification with flame combustion, incineration and landfilling. The landfilling scenario was used as a reference for comparison. The technologies were assessed from ecological and economic points of view. The results are presented in terms of global warming potential, acidification potential, eutrophication potential, consumption of primary energy carriers and welfare costs. From the simulations, gasification followed by catalytic combustion with energy recovery in a combined cycle appeared to be the most competitive technology from an ecological point of view. On the other hand, this alternative was more expensive than incineration. A sensitivity analysis was done regarding electricity prices to show which technology wins at what value of the unit price of electricity (SEK/kW h). Within this study, it was possible to make a comparison both between a combined cycle and a Rankine cycle (a system pair) and at the same time between flame combustion and catalytic combustion (a technology pair). To use gasification just as a treatment technology is not more appealing than incineration, but the possibility of combining gasification with a combined cycle is attractive in terms of electricity production. This research was done in connection with an empirical R and D work on both gasification of waste and catalytic combustion of the gasified waste at the Division of Chemical Technology, Royal Institute of Technology (KTH), Sweden

Technology Games: Using Wittgenstein for Understanding and Evaluating Technology.

Science.gov (United States)

Coeckelbergh, Mark

2017-08-15

In the philosophy of technology after the empirical turn, little attention has been paid to language and its relation to technology. In this programmatic and explorative paper, it is proposed to use the later Wittgenstein, not only to pay more attention to language use in philosophy of technology, but also to rethink technology itself-at least technology in its aspect of tool, technology-in-use. This is done by outlining a working account of Wittgenstein's view of language (as articulated mainly in the Investigations) and by then applying that account to technology-turning around Wittgenstein's metaphor of the toolbox. Using Wittgenstein's concepts of language games and form of life and coining the term 'technology games', the paper proposes and argues for a use-oriented, holistic, transcendental, social, and historical approach to technology which is empirically but also normatively sensitive, and which takes into account implicit knowledge and know-how. It gives examples of interaction with social robots to support the relevance of this project for understanding and evaluating today's technologies, makes comparisons with authors in philosophy of technology such as Winner and Ihde, and sketches the contours of a phenomenology and hermeneutics of technology use that may help us to understand but also to gain a more critical relation to specific uses of concrete technologies in everyday contexts. Ultimately, given the holism argued for, it also promises a more critical relation to the games and forms of life technologies are embedded in-to the ways we do things.

Clean coal technologies in Japan: technological innovation in the coal industry

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-12-15

This brochure reviews the history clean coal technologies (CCT) in Japan and systematically describes the present state of CCT insofar. The brochure contains three parts. Part 1. CCT classifications; Part 2. CCT overview; and Part 3. Future outlook for CCT. The main section is part 2 which includes 1) technologies for coal resources development; 2) coal-fired power generation technologies - combustion technologies and gasification technologies; 3) iron making and general industry technologies; 4) multi-purpose coal utilization technologies - liquefaction technologies, pyrolysis technologies, powdering, fluidization, and co-utilisation technologies, and de-ashing and reforming technologies; 5) Environmental protection technologies - CO{sub 2} recovery technologies; flue gas treatment and gas cleaning technologies, and technologies to effectively use coal has; 6) basic technologies for advanced coal utilization; and 7) co-production systems.

Technology Matters - When new technology reshape innovation

DEFF Research Database (Denmark)

Edwards, Kasper; Pedersen, Jørgen Lindgaard

2004-01-01

Management of innovation is an important issue for firms and being good at this may be the deciding difference between death and survival. This paper ar-gues, based on 12 case studies, that new technology influence the innovative ca-pability of firms and disturbingly the process appears...... not to be managed in the sense that aims, instruments and resources are unclear. It is observed that new technology is adopted with a limited scope and fo-cus – often to solve a particular technical problem e.g. the quality of specifica-tion are too low. For a single reason a new technology is introduced within...... the firm, which over time becomes a source of innovation. However, through adoption of this new technology firms engage in a mu-tual learning and forming process where the firm learn by using the new technol-ogy. When learning, the firm and the new technology is mutually formed as the firm tries to adopt...

Subjective Technology Adaptivity Predicts Technology Use in Old Age.

Science.gov (United States)

Kamin, Stefan T; Lang, Frieder R; Beyer, Anja

2017-01-01

To date, not much is known about the psychological and motivational factors underlying technology use in late life. What are the interindividual determinants that lead older adults to invest in using technological innovations despite the age-related physiological changes that impose challenges on behavioral plasticity in everyday life? This research explores interindividual differences in subjective technology adaptivity - a general technology-related motivational resource that accounts for technology use in late life. More specifically, we investigate the influence of this factor relative to demographic characteristics, personality traits, and functional limitations in a longitudinal sample of community-dwelling older adults. We report results from a paper-and-pencil survey with 136 older adults between 59 and 92 years of age (mean = 71.4, SD = 7.4). Of those participants, 77 participated in a 2-year follow-up. We assessed self-reports of technology use, subjective technology adaptivity, functional limitations, and the personality traits openness to new experiences and neuroticism. Higher levels of subjective technology adaptivity were associated with technology use at the first measurement as well as increased use over the course of 2 years. Subjective technology adaptivity is a significant predictor of technology use in old age. Our findings contribute to improving the understanding of interindividual differences when using technological innovation in late life. Moreover, our findings have implications in the context of user involvement and may contribute to the successful development of innovative technology for older adults. © 2017 S. Karger AG, Basel.

Living Technology

DEFF Research Database (Denmark)

2010-01-01

This book is aimed at anyone who is interested in learning more about living technology, whether coming from business, the government, policy centers, academia, or anywhere else. Its purpose is to help people to learn what living technology is, what it might develop into, and how it might impact...... our lives. The phrase 'living technology' was coined to refer to technology that is alive as well as technology that is useful because it shares the fundamental properties of living systems. In particular, the invention of this phrase was called for to describe the trend of our technology becoming...... increasingly life-like or literally alive. Still, the phrase has different interpretations depending on how one views what life is. This book presents nineteen perspectives on living technology. Taken together, the interviews convey the collective wisdom on living technology's power and promise, as well as its...

Technology integration project: Environmental Restoration Technologies Department Sandia National Laboratories

International Nuclear Information System (INIS)

Williams, C.V.; Burford, T.D.

1996-08-01

Sandia National Laboratories Environmental Restoration Technologies Department is developing environmental restoration technologies through funding form the US Department of Energy's (DOE's) Office of Science and Technology. Initially, this technology development has been through the Mixed Waste Landfill Integrated Demonstration (MWLID). It is currently being developed through the Contaminant Plume containment and Remediation Focus Area, the Landfill Stabilization Focus Area, and the Characterization, Monitoring, and Sensor Cross-Cutting Program. This Technology Integration Project (TIP) was responsible for transferring MWLID-developed technologies for routine use by environmental restoration groups throughout the DOE complex and commercializing these technologies to the private sector. The MWLID's technology transfer/commercialization successes were achieved by involving private industry in development, demonstration, and technology transfer/commercialization activities; gathering and disseminating information about MWLID activities and technologies; and promoting stakeholder and regulatory involvement. From FY91 through FY95, 30 Technical Task Plans (TTPs) were funded. From these TTPs, the MWLID can claim 15 technology transfer/commercialization successes. Another seven technology transfer/commercialization successes are expected. With the changeover to the focus areas, the TIP continued the technology transfer/commercialization efforts begun under the MWLID

Technology integration project: Environmental Restoration Technologies Department Sandia National Laboratories

Energy Technology Data Exchange (ETDEWEB)

Williams, C.V.; Burford, T.D. [Sandia National Labs., Albuquerque, NM (United States). Environmental Restoration Technologies; Allen, C.A. [Tech Reps, Inc., Albuquerque, NM (United States)

1996-08-01

Sandia National Laboratories Environmental Restoration Technologies Department is developing environmental restoration technologies through funding form the US Department of Energy`s (DOE`s) Office of Science and Technology. Initially, this technology development has been through the Mixed Waste Landfill Integrated Demonstration (MWLID). It is currently being developed through the Contaminant Plume containment and Remediation Focus Area, the Landfill Stabilization Focus Area, and the Characterization, Monitoring, and Sensor Cross-Cutting Program. This Technology Integration Project (TIP) was responsible for transferring MWLID-developed technologies for routine use by environmental restoration groups throughout the DOE complex and commercializing these technologies to the private sector. The MWLID`s technology transfer/commercialization successes were achieved by involving private industry in development, demonstration, and technology transfer/commercialization activities; gathering and disseminating information about MWLID activities and technologies; and promoting stakeholder and regulatory involvement. From FY91 through FY95, 30 Technical Task Plans (TTPs) were funded. From these TTPs, the MWLID can claim 15 technology transfer/commercialization successes. Another seven technology transfer/commercialization successes are expected. With the changeover to the focus areas, the TIP continued the technology transfer/commercialization efforts begun under the MWLID.

Technology '90

International Nuclear Information System (INIS)

1991-01-01

The US Department of Energy (DOE) laboratories have a long history of excellence in performing research and development in a number of areas, including the basic sciences, applied-energy technology, and weapons-related technology. Although technology transfer has always been an element of DOE and laboratory activities, it has received increasing emphasis in recent years as US industrial competitiveness has eroded and efforts have increased to better utilize the research and development resources the laboratories provide. This document, Technology '90, is the latest in a series that is intended to communicate some of the many opportunities available for US industry and universities to work with the DOE and its laboratories in the vital activity of improving technology transfer to meet national needs. Technology '90 is divided into three sections: Overview, Technologies, and Laboratories. The Overview section describes the activities and accomplishments of the DOE research and development program offices. The Technologies section provides descriptions of new technologies developed at the DOE laboratories. The Laboratories section presents information on the missions, programs, and facilities of each laboratory, along with a name and telephone number of a technology transfer contact for additional information. Separate papers were prepared for appropriate sections of this report

Sensemaking technologies

DEFF Research Database (Denmark)

Madsen, Charlotte Øland

Research scope: The scope of the project is to study technological implementation processes by using Weick's sensemaking concept (Weick, 1995). The purpose of using a social constructivist approach to investigate technological implementation processes is to find out how new technologies transform......, Orlikowski 2000). Viewing the use of technology as a process of enactment opens up for investigating the social processes of interpreting new technology into the organisation (Orlikowski 2000). The scope of the PhD project will therefore be to gain a deeper understanding of how the enactment of new...... & Brass, 1990; Kling 1991; Orlikowski 2000). It also demonstrates that technology is a flexible variable adapted to the organisation's needs, culture, climate and management philosophy, thus leading to different uses and outcomes of the same technology in different organisations (Barley 1986; 1990...

Technology roadmaps

Energy Technology Data Exchange (ETDEWEB)

Pearson, B. [Natural Resources Canada, Ottawa, ON (Canada). CANMET Energy Technology Centre

2003-07-01

The purpose of a technology road map is to define the state of a current technology, relevant market issues, and future market needs; to develop a plan that industry can follow to provide these new products and services; and to map technology pathways and performance goals for bringing these products and services to market. The three stages (planning, implementation, and reviewing and updating), benefits, and status of the Clean Coal Technology Roadmap are outlined. Action Plan 2000, a $1.7 million 2000 Climate Change Technology and Innovation Program, which uses the technology roadmapping process, is described. The members of the management steering committee for the Clean Coal Technology Roadmap are listed. A flowsheet showing activities until November 2004, when the final clean coal road map is due, is included.

Technological entrepreneurship : technology transfer from academia to new firms

NARCIS (Netherlands)

Prodan, I.

2007-01-01

This doctoral dissertation aims to do the following: 1. Develop the conceptual model of technological entrepreneurship 2. Position technology transfer from academia to new firms in a newly developed conceptual model of technological entrepreneurship 3. Develop the model of technology transfer from

International technology transfer

International Nuclear Information System (INIS)

Kwon, Won Gi

1991-11-01

This book introduces technology progress and economic growth, theoretical consideration of technology transfer, policy and mechanism on technology transfer of a developed country and a developing country, reality of international technology transfer technology transfer and industrial structure in Asia and the pacific region, technology transfer in Russia, China and Eastern Europe, cooperation of science and technology for development of Northeast Asia and strategy of technology transfer of Korea.

Electronic technology

International Nuclear Information System (INIS)

Kim, Jin Su

2010-07-01

This book is composed of five chapters, which introduces electronic technology about understanding of electronic, electronic component, radio, electronic application, communication technology, semiconductor on its basic, free electron and hole, intrinsic semiconductor and semiconductor element, Diode such as PN junction diode, characteristic of junction diode, rectifier circuit and smoothing circuit, transistor on structure of transistor, characteristic of transistor and common emitter circuit, electronic application about electronic equipment, communication technology and education, robot technology and high electronic technology.

Technology in the Classroom: Teachers and Technology--A Technological Divide

Science.gov (United States)

Clarke, Gregory, Sr.; Zagarell, Jesse

2012-01-01

The education system in the United States continues to grapple with adapting to change, especially when it comes to integrating technology in the curriculum. The United States needs to use its resources to stay competitive in the increasingly technological world, particularly in the classroom. Lefebvre, Deaudelin, and Loiselle (2006) posit that…

Educational Technologies in Health Science Libraries: Teaching Technology Skills

Science.gov (United States)

Hurst, Emily J.

2014-01-01

As technology rapidly changes, libraries remain go-to points for education and technology skill development. In academic health sciences libraries, trends suggest librarians provide more training on technology topics than ever before. While education and training have always been roles for librarians, providing technology training on new mobile devices and emerging systems requires class creation and training capabilities that are new to many. To appeal to their users, many health sciences librarians are interested in developing technology-based classes. This column explores the question: what skills are necessary for developing and teaching technology in an academic health sciences library setting? PMID:24528269

Educational technologies in health sciences libraries: teaching technology skills.

Science.gov (United States)

Hurst, Emily J

2014-01-01

As technology rapidly changes, libraries remain go-to points for education and technology skill development. In academic health sciences libraries, trends suggest librarians provide more training on technology topics than ever before. While education and training have always been roles for librarians, providing technology training on new mobile devices and emerging systems requires class creation and training capabilities that are new to many librarians. To appeal to their users, many health sciences librarians are interested in developing technology-based classes. This column explores the question: what skills are necessary for developing and teaching technology in an academic health sciences library setting?

Peripheral blood antigen presenting cell responses in otitis-prone and non-otitis-prone infants.

Science.gov (United States)

Surendran, Naveen; Nicolosi, Ted; Kaur, Ravinder; Pichichero, Michael E

2016-01-01

Stringently defined otitis-prone (sOP) children represent a new classification of the otitis-prone condition. Previous studies showed dysfunction in Ab, B-cell memory and T-cell memory responses. We sought to determine whether there are defects in numbers, phenotype and/or function of professional APC in the peripheral blood of sOP infants. APC phenotypic counts, MHC II expression and intracellular cytokine levels were determined in response to TLR7/8 (R848) stimulation by flow cytometry. Innate immune mRNA expression was measured using RT-PCR and cytokines were measured using Luminex technology. Significant (P otitis-prone (NOP) age-matched infants. No significant differences in APC activation or function were observed. Expression of various TLRs, intracellular signaling molecules and downstream cytokines was also not found to be significantly different between sOP and NOP infants. Higher numbers of APCs in sOP infants suggest the possibility of a persistent mucosal inflammatory status. Transcriptional and cytokine profiles of PBMCs among sOP infants suggest their systemic innate responses are not different compared to NOP infants. © The Author(s) 2015.

Evidence of HLA-DQB1 Contribution to Susceptibility of Dengue Serotype 3 in Dengue Patients in Southern Brazil

Directory of Open Access Journals (Sweden)

Daniela Maria Cardozo

2014-01-01

Full Text Available Dengue infection (DI transmitted by arthropod vectors is the viral disease with the highest incidence throughout the world, an estimated 300 million cases per year. In addition to environmental factors, genetic factors may also influence the manifestation of the disease; as even in endemic areas, only a small proportion of people develop the most serious form. Immune-response gene polymorphisms may be associated with the development of cases of DI. The aim of this study was to determine allele frequencies in the HLA-A, B, C, DRB1, DQA1, and DQB1 loci in a Southern Brazil population with dengue virus serotype 3, confirmed by the ELISA serological method, and a control group. The identification of the HLA alleles was carried out using the SSO genotyping PCR program (One Lambda, based on Luminex technology. In conclusion, this study suggests that DQB1*06:11 allele could act as susceptible factors to dengue virus serotype 3, while HLA-DRB1*11 and DQA1*05:01 could act as resistance factors.

Concordance of Beta-papillomavirus across anogenital and oral anatomic sites of men: The HIM Study.

Science.gov (United States)

Nunes, Emily M; López, Rossana V M; Sudenga, Staci L; Gheit, Tarik; Tommasino, Massimo; Baggio, Maria L; Ferreira, Silvaneide; Galan, Lenice; Silva, Roberto C; Lazcano-Ponce, Eduardo; Giuliano, Anna R; Villa, Luisa L; Sichero, Laura

2017-10-01

We evaluated the concordance between β-HPVs detected in external genital skin, anal canal, and oral cavity specimens collected simultaneously from 717 men that were participating in the multinational HIM Study. Viral genotyping was performed using the Luminex technology. Species- and type-specific concordance was measured using kappa statistics for agreement. Overall, concordance of β-HPVs across sites was low and mainly observed among paired genital/anal canal samples. When grouped by species, solely β-4 HPVs showed moderate concordance in genital/anal pairs (κ = 0.457), which could be attributed to the substantial concordance of HPV-92 in men from Brazil and Mexico (κ > 0.610). β-HPV type concordance was higher in Mexico, where HPV-19 was consistently concordant in all anatomic site combinations. Our analysis indicates that type-specific concordance across sites is limited to few viral types; however, these infections seem to occur more often than would be expected by chance, suggesting that although rare, there is agreement among sites. Copyright © 2017 Elsevier Inc. All rights reserved.

Technology Transfer Issues and a New Technology Transfer Model

Science.gov (United States)

Choi, Hee Jun

2009-01-01

The following are major issues that should be considered for efficient and effective technology transfer: conceptions of technology, technological activity and transfer, communication channels, factors affecting transfer, and models of transfer. In particular, a well-developed model of technology transfer could be used as a framework for…

Technology Acceptance of Augmented Reality and Wearable Technologies

NARCIS (Netherlands)

Wild, Fridolin; Klemke, Roland; Lefrere, Paul; Fominykh, Mikhail; Kuula, Timo

2017-01-01

Augmented Reality and Wearables are the recent media and computing technologies, similar, but different from established technologies, even mobile computing and virtual reality. Numerous proposals for measuring technology acceptance exist, but have not been applied, nor fine-tuned to such

Technological Literacy Education and Technological and Vocational Education in Taiwan

Science.gov (United States)

Lee, Lung-Sheng Steven

2010-01-01

Technology education in Taiwan is categorized into the following two types: (1) technological literacy education (TLE)--the education for all people to become technological literates; and (2) technological specialty education (TSE)--the education for specific people to become technicians and professionals for technology-related jobs. This paper…

Laser Technology.

Science.gov (United States)

Gauger, Robert

1993-01-01

Describes lasers and indicates that learning about laser technology and creating laser technology activities are among the teacher enhancement processes needed to strengthen technology education. (JOW)

Projecting technology change to improve space technology planning and systems management

Science.gov (United States)

Walk, Steven Robert

2011-04-01

Projecting technology performance evolution has been improving over the years. Reliable quantitative forecasting methods have been developed that project the growth, diffusion, and performance of technology in time, including projecting technology substitutions, saturation levels, and performance improvements. These forecasts can be applied at the early stages of space technology planning to better predict available future technology performance, assure the successful selection of technology, and improve technology systems management strategy. Often what is published as a technology forecast is simply scenario planning, usually made by extrapolating current trends into the future, with perhaps some subjective insight added. Typically, the accuracy of such predictions falls rapidly with distance in time. Quantitative technology forecasting (QTF), on the other hand, includes the study of historic data to identify one of or a combination of several recognized universal technology diffusion or substitution patterns. In the same manner that quantitative models of physical phenomena provide excellent predictions of system behavior, so do QTF models provide reliable technological performance trajectories. In practice, a quantitative technology forecast is completed to ascertain with confidence when the projected performance of a technology or system of technologies will occur. Such projections provide reliable time-referenced information when considering cost and performance trade-offs in maintaining, replacing, or migrating a technology, component, or system. This paper introduces various quantitative technology forecasting techniques and illustrates their practical application in space technology and technology systems management.

Technology for whom: an adequate technology

Energy Technology Data Exchange (ETDEWEB)

Alva, E N

1978-01-01

The concept of human settlements technology (HST) is described and applied to the unique needs of developing countries having different cultural and climatic conditions. These countries are experiencing rapid growth in population, and appropriate technology should not be assumed to be the same as that used in industrialized counties in temperate climates. A new world order is asking that emerging countries define their own living patterns and determine which technologies are suitable for their whole population's needs rather than limiting modern technology to an elite. The author describes how unconventional, but creative, energy can be tapped for housing construction that is low-cost, self-sufficient, and easy to service. A plurality of life styles in tune with the ecosystem could be adopted, but this will require politicians and architects to approach eco-design as a creative rather than imitative process.

Human Spaceflight Technology Needs - A Foundation for JSC's Technology Strategy

Science.gov (United States)

Stecklein, Jonette M.

2013-01-01

Human space exploration has always been heavily influenced by goals to achieve a specific mission on a specific schedule. This approach drove rapid technology development, the rapidity of which adds risks as well as provides a major driver for costs and cost uncertainty. The National Aeronautics and Space Administration (NASA) is now approaching the extension of human presence throughout the solar system by balancing a proactive yet less schedule-driven development of technology with opportunistic scheduling of missions as the needed technologies are realized. This approach should provide cost effective, low risk technology development that will enable efficient and effective manned spaceflight missions. As a first step, the NASA Human Spaceflight Architecture Team (HAT) has identified a suite of critical technologies needed to support future manned missions across a range of destinations, including in cis-lunar space, near earth asteroid visits, lunar exploration, Mars moons, and Mars exploration. The challenge now is to develop a strategy and plan for technology development that efficiently enables these missions over a reasonable time period, without increasing technology development costs unnecessarily due to schedule pressure, and subsequently mitigating development and mission risks. NASA's Johnson Space Center (JSC), as the nation s primary center for human exploration, is addressing this challenge through an innovative approach in allocating Internal Research and Development funding to projects. The HAT Technology Needs (TechNeeds) Database has been developed to correlate across critical technologies and the NASA Office of Chief Technologist Technology Area Breakdown Structure (TABS). The TechNeeds Database illuminates that many critical technologies may support a single technical capability gap, that many HAT technology needs may map to a single TABS technology discipline, and that a single HAT technology need may map to multiple TABS technology

Human spaceflight technology needs-a foundation for JSC's technology strategy

Science.gov (United States)

Stecklein, J. M.

Human space exploration has always been heavily influenced by goals to achieve a specific mission on a specific schedule. This approach drove rapid technology development, the rapidity of which added risks and became a major driver for costs and cost uncertainty. The National Aeronautics and Space Administration (NASA) is now approaching the extension of human presence throughout the solar system by balancing a proactive yet less schedule-driven development of technology with opportunistic scheduling of missions as the needed technologies are realized. This approach should provide cost effective, low risk technology development that will enable efficient and effective manned spaceflight missions. As a first step, the NASA Human Spaceflight Architecture Team (HAT) has identified a suite of critical technologies needed to support future manned missions across a range of destinations, including in cis-lunar space, near earth asteroid visits, lunar exploration, Mars moons, and Mars exploration. The challenge now is to develop a strategy and plan for technology development that efficiently enables these missions over a reasonable time period, without increasing technology development costs unnecessarily due to schedule pressure, and subsequently mitigating development and mission risks. NASA's Johnson Space Center (JSC), as the nation's primary center for human exploration, is addressing this challenge through an innovative approach in allocating Internal Research and Development funding to projects. The HAT Technology Needs (Tech Needs) Database has been developed to correlate across critical technologies and the NASA Office of Chief Technologist Technology Area Breakdown Structure (TABS). The TechNeeds Database illuminates that many critical technologies may support a single technical capability gap, that many HAT technology needs may map to a single TABS technology discipline, and that a single HAT technology need may map to multiple TABS technology disciplines. Th

Examining Engineering & Technology Students' Acceptance of Network Virtualization Technology Using the Technology Acceptance Model

Science.gov (United States)

Yousif, Wael K.

2010-01-01

This causal and correlational study was designed to extend the Technology Acceptance Model (TAM) and to test its applicability to Valencia Community College (VCC) Engineering and Technology students as the target user group when investigating the factors influencing their decision to adopt and to utilize VMware as the target technology. In…

The Technological Dimension of Educational Technology in Europe

Science.gov (United States)

Dimitriadis, Yannis

2012-01-01

This article describes some of the main technological trends and issues of the European landscape of research and innovation in educational technology. Although several innovative technologies (tools, architectures, platforms, or approaches) emerge, such as intelligent support to personalization, collaboration or adaptation in mobile, game-based,…

Technology Catalogue

International Nuclear Information System (INIS)

1994-02-01

The Department of Energy's Office of Environmental Restoration and Waste Management (EM) is responsible for remediating its contaminated sites and managing its waste inventory in a safe and efficient manner. EM's Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste management programs within EM's Office of Environmental Restoration and Office of Waste Management. The purpose of the Technology Catalogue is to provide performance data on OTD-developed technologies to scientists and engineers assessing and recommending technical solutions within the Department's clean-up and waste management programs, as well as to industry, other federal and state agencies, and the academic community. OTD's applied research and demonstration activities are conducted in programs referred to as Integrated Demonstrations (IDs) and Integrated Programs (IPs). The IDs test and evaluate.systems, consisting of coupled technologies, at specific sites to address generic problems, such as the sensing, treatment, and disposal of buried waste containers. The IPs support applied research activities in specific applications areas, such as in situ remediation, efficient separations processes, and site characterization. The Technology Catalogue is a means for communicating the status. of the development of these innovative technologies. The FY93 Technology Catalogue features technologies successfully demonstrated in the field through IDs and sufficiently mature to be used in the near-term. Technologies from the following IDs are featured in the FY93 Technology Catalogue: Buried Waste ID (Idaho National Engineering Laboratory, Idaho); Mixed Waste Landfill ID (Sandia National Laboratories, New Mexico); Underground Storage Tank ID (Hanford, Washington); Volatile organic compound (VOC) Arid ID (Richland, Washington); and VOC Non-Arid ID (Savannah River Site, South Carolina)

The Center for Environmental Technology Innovative Technology Screening Process

International Nuclear Information System (INIS)

Bertrand, C.M.

1995-02-01

The Center for Environmental Technology's (CET) mission is to provide a fully integrated system for accelerated evaluation, development, commercialization, and public acceptance of creative environmental solutions which match the foremost demands in today's environmentally sensitive world. In short, CET will create a means to provide quick, effective solutions for environmental needs. To meet this mission objective, CET has created a unique and innovative approach to eliminating the usual barriers in developing and testing environmental technologies. The approach paves the way for these emerging, cutting-edge technologies by coordinating environmental restoration and waste management activities of industry, universities, and the government to: efficiently and effectively transfer technology to these users, provide market-driven, cost-effective technology programs to the public and DOE, and aid in developing innovative ideas by initiating efforts between DOE facilities and private industry. The central part to this mission is selecting and evaluating specific innovative technologies for demonstration and application at United States Department of Energy (DOE) installations. The methodology and criteria used for this selection, which is called the CET Innovative Technology Screening Process, is the subject of this paper. The selection criteria used for the screening process were modeled after other DOE technology transfer programs and were further developed by CET's Technology Screening and Evaluation Board (TSEB). The process benefits both CET and the proposing vendors by providing objective selection procedures based on predefined criteria. The selection process ensures a rapid response to proposing vendors, all technologies will have the opportunity to enter the selection process, and all technologies are evaluated on the same scale and with identical criteria

NASA Technology Applications Team: Commercial applications of aerospace technology

Science.gov (United States)

1994-01-01

The Research Triangle Institute (RTI) Team has maintained its focus on helping NASA establish partnerships with U.S. industry for dual use development and technology commercialization. Our emphasis has been on outcomes, such as licenses, industry partnerships and commercialization of technologies, that are important to NASA in its mission of contributing to the improved competitive position of U.S. industry. The RTI Team has been successful in the development of NASA/industry partnerships and commercialization of NASA technologies. RTI ongoing commitment to quality and customer responsiveness has driven our staff to continuously improve our technology transfer methodologies to meet NASA's requirements. For example, RTI has emphasized the following areas: (1) Methodology For Technology Assessment and Marketing: RTI has developed and implemented effective processes for assessing the commercial potential of NASA technologies. These processes resulted from an RTI study of best practices, hands-on experience, and extensive interaction with the NASA Field Centers to adapt to their specific needs. (2) Effective Marketing Strategies: RTI surveyed industry technology managers to determine effective marketing tools and strategies. The Technology Opportunity Announcement format and content were developed as a result of this industry input. For technologies with a dynamic visual impact, RTI has developed a stand-alone demonstration diskette that was successful in developing industry interest in licensing the technology. And (3) Responsiveness to NASA Requirements: RTI listened to our customer (NASA) and designed our processes to conform with the internal procedures and resources at each NASA Field Center and the direction provided by NASA's Agenda for Change. This report covers the activities of the Research Triangle Institute Technology Applications Team for the period 1 October 1993 through 31 December 1994.

Assistive Technology

Science.gov (United States)

... Page Resize Text Printer Friendly Online Chat Assistive Technology Assistive technology (AT) is any service or tool that helps ... be difficult or impossible. For older adults, such technology may be a walker to improve mobility or ...

Advanced Reactor Technologies - Regulatory Technology Development Plan (RTDP)

Energy Technology Data Exchange (ETDEWEB)

Moe, Wayne L. [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2017-08-23

This DOE-NE Advanced Small Modular Reactor (AdvSMR) regulatory technology development plan (RTDP) will link critical DOE nuclear reactor technology development programs to important regulatory and policy-related issues likely to impact a “critical path” for establishing a viable commercial AdvSMR presence in the domestic energy market. Accordingly, the regulatory considerations that are set forth in the AdvSMR RTDP will not be limited to any one particular type or subset of advanced reactor technology(s) but rather broadly consider potential regulatory approaches and the licensing implications that accompany all DOE-sponsored research and technology development activity that deal with commercial non-light water reactors. However, it is also important to remember that certain “minimum” levels of design and safety approach knowledge concerning these technology(s) must be defined and available to an extent that supports appropriate pre-licensing regulatory analysis within the RTDP. Final resolution to advanced reactor licensing issues is most often predicated on the detailed design information and specific safety approach as documented in a facility license application and submitted for licensing review. Because the AdvSMR RTDP is focused on identifying and assessing the potential regulatory implications of DOE-sponsored reactor technology research very early in the pre-license application development phase, the information necessary to support a comprehensive regulatory analysis of a new reactor technology, and the resolution of resulting issues, will generally not be available. As such, the regulatory considerations documented in the RTDP should be considered an initial “first step” in the licensing process which will continue until a license is issued to build and operate the said nuclear facility. Because a facility license application relies heavily on the data and information generated by technology development studies, the anticipated regulatory

Advanced Reactor Technology -- Regulatory Technology Development Plan (RTDP)

Energy Technology Data Exchange (ETDEWEB)

Moe, Wayne Leland [Idaho National Lab. (INL), Idaho Falls, ID (United States)

2015-05-01

This DOE-NE Advanced Small Modular Reactor (AdvSMR) regulatory technology development plan (RTDP) will link critical DOE nuclear reactor technology development programs to important regulatory and policy-related issues likely to impact a “critical path” for establishing a viable commercial AdvSMR presence in the domestic energy market. Accordingly, the regulatory considerations that are set forth in the AdvSMR RTDP will not be limited to any one particular type or subset of advanced reactor technology(s) but rather broadly consider potential regulatory approaches and the licensing implications that accompany all DOE-sponsored research and technology development activity that deal with commercial non-light water reactors. However, it is also important to remember that certain “minimum” levels of design and safety approach knowledge concerning these technology(s) must be defined and available to an extent that supports appropriate pre-licensing regulatory analysis within the RTDP. Final resolution to advanced reactor licensing issues is most often predicated on the detailed design information and specific safety approach as documented in a facility license application and submitted for licensing review. Because the AdvSMR RTDP is focused on identifying and assessing the potential regulatory implications of DOE-sponsored reactor technology research very early in the pre-license application development phase, the information necessary to support a comprehensive regulatory analysis of a new reactor technology, and the resolution of resulting issues, will generally not be available. As such, the regulatory considerations documented in the RTDP should be considered an initial “first step” in the licensing process which will continue until a license is issued to build and operate the said nuclear facility. Because a facility license application relies heavily on the data and information generated by technology development studies, the anticipated regulatory

Nano technologies, technologies converging and potential biomedical applications

International Nuclear Information System (INIS)

Capuano, V.

2005-01-01

The applications of nano technology to biology and medicine appear really promising for diagnostics, for various therapeutic approaches and in medical instrumentations. The growing synergism among nano technology, biotechnology, information technology and cognitive sciences, their convergence (NBIC) from the nano scale, could involve on next decades great changes in medicine, from a reactive to a predictive and preventive approach. It is expected that NBIC converging technologies could achieve tremendous improvements in human abilities and enhance societal achievement of related social and ethical implications, in the framework of a constant dialogue between science and society [it

THE CONNECTION BETWEEN MANAGEMENT AND TECHNOLOGY AND THE TECHNOLOGICAL MANAGEMENT

Directory of Open Access Journals (Sweden)

RAREŞ MUNTEANU

2010-01-01

Full Text Available In a new approach, the technology is taken into consideration as a resource of the business, in addition to the four traditional resources: material resources, financial resources, human resources and informational resources. This makes important for the managers to have solid technological knowledge, in addition to the economics. The research regarding the technological management (or the management of technology - MOT is lead by IAMOT (International Association in Management of Technology. There are a lot of connections between the technology on one side and the finances, the human resources, the marketing, the operational management on the other side. In our era the technology is more and more advanced and all the activities are strongly connected to it.

Trends in Health Information Technology Safety: From Technology-Induced Errors to Current Approaches for Ensuring Technology Safety

Science.gov (United States)

2013-01-01

Objectives Health information technology (HIT) research findings suggested that new healthcare technologies could reduce some types of medical errors while at the same time introducing classes of medical errors (i.e., technology-induced errors). Technology-induced errors have their origins in HIT, and/or HIT contribute to their occurrence. The objective of this paper is to review current trends in the published literature on HIT safety. Methods A review and synthesis of the medical and life sciences literature focusing on the area of technology-induced error was conducted. Results There were four main trends in the literature on technology-induced error. The following areas were addressed in the literature: definitions of technology-induced errors; models, frameworks and evidence for understanding how technology-induced errors occur; a discussion of monitoring; and methods for preventing and learning about technology-induced errors. Conclusions The literature focusing on technology-induced errors continues to grow. Research has focused on the defining what an error is, models and frameworks used to understand these new types of errors, monitoring of such errors and methods that can be used to prevent these errors. More research will be needed to better understand and mitigate these types of errors. PMID:23882411

Copper Cable Recycling Technology. Innovative Technology Summary Report

International Nuclear Information System (INIS)

2001-01-01

The United States Department of Energy (DOE) continually seeks safer and more cost-effective technologies for use in deactivation and decommissioning (D and D) of nuclear facilities. The Deactivation and Decommissioning Focus Area (DDFA) of the DOE's Office of Science and Technology (OST) sponsors large-scale demonstration and deployment projects (LSDDPs). At these LSDDPs, developers and vendors of improved or innovative technologies showcase products that are potentially beneficial to the DOE's projects and to others in the D and D community. Benefits sought include decreased health and safety risks to personnel and the environment, increased productivity, and decreased costs of operation. The Idaho National Engineering and Environmental Laboratory (INEEL) generated a list of statements defining specific needs and problems where improved technology could be incorporated into ongoing D and D tasks. One such need is to reduce the volume of waste copper wire and cable generated by D and D. Deactivation and decommissioning activities of nuclear facilities generates hundreds of tons of contaminated copper cable, which are sent to radioactive waste disposal sites. The Copper Cable Recycling Technology separates the clean copper from contaminated insulation and dust materials in these cables. The recovered copper can then be reclaimed and, more importantly, landfill disposal volumes can be reduced. The existing baseline technology for disposing radioactively contaminated cables is to package the cables in wooden storage boxes and dispose of the cables in radioactive waste disposal sites. The Copper Cable Recycling Technology is applicable to facility decommissioning projects at many Department of Energy (DOE) nuclear facilities and commercial nuclear power plants undergoing decommissioning activities. The INEEL Copper Cable Recycling Technology Demonstration investigated the effectiveness and efficiency to recycle 13.5 tons of copper cable. To determine the effectiveness

Educational technology and the new technologies

NARCIS (Netherlands)

Verhagen, Pleunes Willem; Plomp, T.

1989-01-01

Like everywhere in our culture, new technologies gradually penetrate the field of education. This may be seen as a problem area, which asks for appropriate, actions by teachers, curriculum experts, instructional designers and others. As "technology" seems to be the main issue,one may quation whether

Soulful Technologies

DEFF Research Database (Denmark)

Fausing, Bent

2010-01-01

Samsung introduced in 2008 a mobile phone called "Soul" made with a human touch and including itself a "magic touch". Through the analysis of a Nokia mobile phone TV-commercials I want to examine the function and form of digital technology in everyday images. The mobile phone and its digital camera...... and other devices are depicted by everyday aesthetics as capable of producing a unique human presence and interaction. The medium, the technology is a necessary helper of this very special and lost humanity. Without the technology, no special humanity, no soul - such is the prophecy. This personification...... or anthropomorphism is important for the branding of new technology. Technology is seen as creating a techno-transcendence towards a more qualified humanity which is in contact with fundamental human values like intuition, vision, and sensing; all the qualities that technology, industrialization, and rationalization...

Sport Technology

CSIR Research Space (South Africa)

Kirkbride, T

2007-11-01

Full Text Available Technology is transforming the games themselves and at times with dire consequences. Tony Kirkbride, Head: CSIR Technology Centre said there are a variety of sports technologies and there have been advances in material sciences and advances...

Technology Transfer Report

Science.gov (United States)

2000-01-01

Since its inception, Goddard has pursued a commitment to technology transfer and commercialization. For every space technology developed, Goddard strives to identify secondary applications. Goddard then provides the technologies, as well as NASA expertise and facilities, to U.S. companies, universities, and government agencies. These efforts are based in Goddard's Technology Commercialization Office. This report presents new technologies, commercialization success stories, and other Technology Commercialization Office activities in 1999.

How does technological regime affect performance of technology development projects?

NARCIS (Netherlands)

Song, Michael; Hooshangi, Soheil; Zhao, Y. Lisa; Halman, Johannes I.M.

2014-01-01

In this study, we examine how technological regime affects the performance of technology development projects (i.e., project quality, sales, and profit). Technological regime is defined as the set of attributes of a technological environment where the innovative activities of firms take place.

Managing Technology Resourcefully: Part I--Technology and Instruction

Science.gov (United States)

Weeks, Richard

2009-01-01

The transformative powers of digital technology to improve student learning and the resulting effect of that technology to make the business of education more cost-effective are two of the more exciting dynamics in schooling today. Before the current school year ends, new products and upgrades will be available to replace much of the technology.…

Technology Tiers

DEFF Research Database (Denmark)

Karlsson, Christer

2015-01-01

A technology tier is a level in a product system: final product, system, subsystem, component, or part. As a concept, it contrasts traditional “vertical” special technologies (for example, mechanics and electronics) and focuses “horizontal” feature technologies such as product characteristics...

Industrial Arts Test Development, Book III. Resource Items for Graphics Technology, Power Technology, Production Technology.

Science.gov (United States)

New York State Education Dept., Albany.

This booklet is designed to assist teachers in developing examinations for classroom use. It is a collection of 955 objective test questions, mostly multiple choice, for industrial arts students in the three areas of graphics technology, power technology, and production technology. Scoring keys are provided. There are no copyright restrictions,…

Earthing Technology

NARCIS (Netherlands)

Blok, Vincent

2017-01-01

In this article, we reflect on the conditions under which new technologies emerge in the Anthropocene and raise the question of how to conceptualize sustainable technologies therein. To this end, we explore an eco-centric approach to technology development, called biomimicry. We discuss opposing

Advanced Reactor Technology -- Regulatory Technology Development Plan (RTDP)

International Nuclear Information System (INIS)

Moe, Wayne Leland

2015-01-01

This DOE-NE Advanced Small Modular Reactor (AdvSMR) regulatory technology development plan (RTDP) will link critical DOE nuclear reactor technology development programs to important regulatory and policy-related issues likely to impact a ''critical path'' for establishing a viable commercial AdvSMR presence in the domestic energy market. Accordingly, the regulatory considerations that are set forth in the AdvSMR RTDP will not be limited to any one particular type or subset of advanced reactor technology(s) but rather broadly consider potential regulatory approaches and the licensing implications that accompany all DOE-sponsored research and technology development activity that deal with commercial non-light water reactors. However, it is also important to remember that certain ''minimum'' levels of design and safety approach knowledge concerning these technology(s) must be defined and available to an extent that supports appropriate pre-licensing regulatory analysis within the RTDP. Final resolution to advanced reactor licensing issues is most often predicated on the detailed design information and specific safety approach as documented in a facility license application and submitted for licensing review. Because the AdvSMR RTDP is focused on identifying and assessing the potential regulatory implications of DOE-sponsored reactor technology research very early in the pre-license application development phase, the information necessary to support a comprehensive regulatory analysis of a new reactor technology, and the resolution of resulting issues, will generally not be available. As such, the regulatory considerations documented in the RTDP should be considered an initial ''first step'' in the licensing process which will continue until a license is issued to build and operate the said nuclear facility. Because a facility license application relies heavily on the data and information generated by

The Ultimate Technology

DEFF Research Database (Denmark)

Riis, Søren

2013-01-01

One of the most influential philosophers of the 20th century, Martin Heidegger (1889-1976), died prior to the remarkable cloning of the sheep Dolly and before Dr. Venter started his experiments on creating synthetic life, and he never explicitly discussed living technologies. However, by reinterp......One of the most influential philosophers of the 20th century, Martin Heidegger (1889-1976), died prior to the remarkable cloning of the sheep Dolly and before Dr. Venter started his experiments on creating synthetic life, and he never explicitly discussed living technologies. However......, by reinterpreting his notion of "modern technology," this article shows how it is possible to philosophically assess living technologies and to recognize ways in which Heidegger anticipated this phenomenon with his notion of cybernetics. The interpretation elucidates the fundamental process of technology becoming...... living and simultaneously presents living technology as the ultimate technology. The thesis of this article is that living technology is not just one more technology; rather, it is the perfection of technology as understood by Aristotle. Aristotle's thinking is in this way a key example of a profound...

Technology transfer 1994

Energy Technology Data Exchange (ETDEWEB)

1994-01-01

This document, Technology Transfer 94, is intended to communicate that there are many opportunities available to US industry and academic institutions to work with DOE and its laboratories and facilities in the vital activity of improving technology transfer to meet national needs. It has seven major sections: Introduction, Technology Transfer Activities, Access to Laboratories and Facilities, Laboratories and Facilities, DOE Office, Technologies, and an Index. Technology Transfer Activities highlights DOE`s recent developments in technology transfer and describes plans for the future. Access to Laboratories and Facilities describes the many avenues for cooperative interaction between DOE laboratories or facilities and industry, academia, and other government agencies. Laboratories and Facilities profiles the DOE laboratories and facilities involved in technology transfer and presents information on their missions, programs, expertise, facilities, and equipment, along with data on whom to contact for additional information on technology transfer. DOE Offices summarizes the major research and development programs within DOE. It also contains information on how to access DOE scientific and technical information. Technologies provides descriptions of some of the new technologies developed at DOE laboratories and facilities.

New Technologies of Information and Communications from a Science, Technology and Society.

Directory of Open Access Journals (Sweden)

Manuel Martín Rodríguez

2012-03-01

Full Text Available With this work we want to make a short analysis of the New Technologies of Information and Communications in basic aspects of interest to technology education, refered to it from a Science Technology and Society (CTS. Based on different conceptions of technology and technological literacy, considers issues such as beliefs about the nature of technological knowledge, relationships and differences between scientific knowledge and the interactions between technology and society, the interests and attitudes of teachers and students to technology and learning from the perspective of education and, finally, various approaches to technology education programs following the approaches Science, Technology and Society.

The Impact of In-Service Technology Training Programmes on Technology Teachers

Science.gov (United States)

Gumbo, Mishack; Makgato, Moses; Muller, Helene

2012-01-01

The aim of this paper is to assess the impact the Advanced Certificate in Education (ACE) in-service technology training program has on technology teachers' knowledge and understanding of technology. The training of technology teachers is an initiative toward teachers' professional development within the mathematics, science, and technology sphere…

Chemical Composition and Anti-Inflammatory Effect of Ethanolic Extract of Brazilian Green Propolis on Activated J774A.1 Macrophages

Directory of Open Access Journals (Sweden)

Ewelina Szliszka

2013-01-01

Full Text Available The aim of this study was to investigate the chemical composition and anti-inflammatory effect of ethanolic extract of Brazilian green propolis (EEP-B on LPS + IFN-γ or PMA stimulated J774A.1 macrophages. The identification and quantification of phenolic compounds in green propolis extract were performed using HPLC-DAD and UPLC-Q-TOF-MS methods. The cell viability was evaluated by MTT and LDH assays. The radical scavenging ability was determined using DPPH• and ABTS•+. ROS and RNS generation was analyzed by chemiluminescence. NO concentration was detected by the Griess reaction. The release of various cytokines by activated J774A.1 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology. Artepillin C, kaempferide, and their derivatives were the main phenolics found in green propolis. At the tested concentrations, the EEP-B did not decrease the cell viability and did not cause the cytotoxicity. EEP-B exerted strong antioxidant activity and significantly inhibited the production of ROS, RNS, NO, cytokine IL-1α, IL-1β, IL-4, IL-6, IL-12p40, IL-13, TNF-α, G-CSF, GM-CSF, MCP-1, MIP-1α, MIP-1β, and RANTES in stimulated J774A.1 macrophages. Our findings provide new insights for understanding the anti-inflammatory mechanism of action of Brazilian green propolis extract and support its application in complementary and alternative medicine.

Increased Blood Levels of Growth Factors, Proinflammatory Cytokines, and Th17 Cytokines in Patients with Newly Diagnosed Type 1 Diabetes.

Science.gov (United States)

Alnek, Kristi; Kisand, Kalle; Heilman, Kaire; Peet, Aleksandr; Varik, Karin; Uibo, Raivo

2015-01-01

The production of several cytokines could be dysregulated in type 1 diabetes (T1D). In particular, the activation of T helper (Th) type 1 (Th1) cells has been proposed to underlie the autoimmune pathogenesis of the disease, although roles for inflammatory processes and the Th17 pathway have also been shown. Nevertheless, despite evidence for the role of cytokines before and at the onset of T1D, the corresponding findings are inconsistent across studies. Moreover, conflicting data exist regarding the blood cytokine levels in T1D patients. The current study was performed to investigate genetic and autoantibody markers in association with the peripheral blood cytokine profiles by xMap multiplex technology in newly diagnosed young T1D patients and age-matched healthy controls. The onset of young-age T1D was characterized by the upregulation of growth factors, including granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-7, the proinflammatory cytokine IL-1β (but not IL-6 or tumor necrosis factor [TNF]-α), Th17 cytokines, and the regulatory cytokines IL-10 and IL-27. Ketoacidosis and autoantibodies (anti-IA-2 and -ZnT8), but not human leukocyte antigen (HLA) genotype, influenced the blood cytokine levels. These findings broaden the current understanding of the dysregulation of systemic levels of several key cytokines at the young-age onset of T1D and provide a further basis for the development of novel immunoregulatory treatments in this disease.

The Impact of Experience and Technology Change on Task-Technology Fit of a Collaborative Technology

Science.gov (United States)

Iversen, Jakob H.; Eierman, Michael A.

2018-01-01

This study continues a long running effort to examine collaborative writing and editing tools and the factors that impact Task-Technology Fit and Technology Acceptance. Previous studies found that MS Word/email performed better than technologies such as Twiki, Google Docs, and Office Live. The current study seeks to examine specifically the impact…

Plasma technology directory

International Nuclear Information System (INIS)

Ward, P.P.; Dybwad, G.L.

1995-01-01

The Plasma Technology Directory has two main goals: (1) promote, coordinate, and share plasma technology experience and equipment within the Department of Energy; and (2) facilitate technology transfer to the commercial sector where appropriate. Personnel are averaged first by Laboratory and next by technology area. The technology areas are accelerators, cleaning and etching deposition, diagnostics, and modeling

Understanding Technology Literacy: A Framework for Evaluating Educational Technology Integration

Science.gov (United States)

Davies, Randall S.

2011-01-01

Federal legislation in the United States currently mandates that technology be integrated into school curricula because of the popular belief that learning is enhanced through the use of technology. The challenge for educators is to understand how best to teach with technology while developing the technological expertise of their students. This…

Excimer Laser Technology

CERN Document Server

Basting, Dirk

2005-01-01

This comprehensive survey on Excimer Lasers investigates the current range of the technology, applications and devices of this commonly used laser source, as well as the future of new technologies, such as F2 laser technology. Additional chapters on optics, devices and laser systems complete this compact handbook. A must read for laser technology students, process application researchers, engineers or anyone interested in excimer laser technology. An effective and understandable introduction to the current and future status of excimer laser technology.

"Obligatory Technologies": Explaining Why People Feel Compelled to Use Certain Technologies

Science.gov (United States)

Chandler, Jennifer A.

2012-01-01

The ideas of technological determinism and the autonomy of technology are long-standing and widespread. This article explores why the use of certain technologies is perceived to be obligatory, thus fueling the fatalism of technological determinism and undermining our sense of freedom vis-a-vis the use of technologies. Three main mechanisms that…

Sensemaking technology

DEFF Research Database (Denmark)

Madsen, Charlotte Øland

Research objective: The object of the LOK research project is to gain a better understanding of the technological strategic processes in organisations by using the concept/metaphor of sensemaking. The project will investigate the technological strategies in organisations in order to gain a deeper...... understanding of the cognitive competencies and barriers towards implementing new technology in organisations. The research will therefore concentrate on researching the development process in the organisation's perception of the external environmental elements of customers, suppliers, competitors, internal...... and external technology and legislation and the internal environmental elements of structure, power relations and political arenas. All of these variables have influence on which/how technologies are implemented thus creating different outcomes all depending on the social dynamics that are triggered by changes...

Conventional Oil and Gas Technologies. IEA ETSAP technology brief P01

Energy Technology Data Exchange (ETDEWEB)

Seljom, Pernille [IFE (Norway)], E-mail: pernille.seljom@ife.no

2010-05-15

This technology brief on conventional oil and gas technologies is part of a series produced by the IEA ETSAP agreement called the energy technology data source (E-Tech-DS). The E-Tech-DS series consists of a number of 5-10 page technology briefs similar to the IEA Energy Technology Essentials. Based on the data collected for the models that the Energy Technology Systems Analysis Programme (ETSAP) is known for, ETSAP also prepares technology briefs, called E-TechDS. The E-TechDS briefs are standardized presentations of basic information (process, status, performance, costs, potential, and barriers) for key energy technology clusters. Each brief includes an overview of the technology, charts and graphs, and a summary data table, and usually ending with some key references and further information. The E TechDS briefs are intended to offer essential, reliable and quantitative information to energy analysts, experts, policymakers, investors and media from both developed and developing countries. This specific brief focuses on the state of oil and gas exploration, development, and production/recovery technologies.

Science, technology, and pedagogy: Exploring secondary science teachers' effective uses of technology

Science.gov (United States)

Guzey, Siddika Selcen

Technology has become a vital part of our professional and personal lives. Today we cannot imagine living without many technological tools such as computers. For the last two decades technology has become inseparable from several areas, such as science. However, it has not been fully integrated into the field of education. The integration of technology in teaching and learning is still challenging even though there has been a historical growth of Internet access and available technology tools in schools (U.S. Department of Education, National Center for Education Statistics, 2006). Most teachers have not incorporated technology into their teaching for various reasons such as lack of knowledge of educational technology tools and having unfavorable beliefs about the effectiveness of technology on student learning. In this study, three beginning science teachers who have achieved successful technology integration were followed to investigate how their beliefs, knowledge, and identity contribute to their uses of technology in their classroom instruction. Extensive classroom observations and interviews were conducted. The findings demonstrate that the participating teachers are all intrinsically motivated to use technology in their teaching and this motivation allows them to enjoy using technology in their instruction and keeps them engaged in technology use. These teachers use a variety of technology tools in their instruction while also allowing students to use them, and they posit a belief set in favor of technology. The major findings of the study are displayed in a model which indicates that teachers' use of technology in classroom instruction was constructed jointly by their technology, pedagogy, and content knowledge; identity; beliefs; and the resources that are available to them and that the internalization of the technology use comes from reflection. The study has implications for teachers, teacher educators, and school administrators for successful technology

Mini Technology Manual for Schools: An Introduction to Technology Integration

Science.gov (United States)

Grismore, Brian A.

2012-01-01

The purpose of this manual is to assist school leaders in beginning or developing the use of educational technology within their school or district. The manual covers topics: 1) advantages of educational technology; 2) types of technology used for learning and teaching; 3) how to make good decisions about the use of technology in schools; 4) the…

Evaluating Technology Resistance and Technology Satisfaction on Students' Performance

Science.gov (United States)

Norzaidi, Mohd Daud; Salwani, Mohamed Intan

2009-01-01

Purpose: Using the extended task-technology fit (TTF) model, this paper aims to examine technology resistance, technology satisfaction and internet usage on students' performance. Design/methodology/approach: The study was conducted at Universiti Teknologi MARA, Johor, Malaysia and questionnaires were distributed to 354 undergraduate students.…

Digital Actuator Technology

Energy Technology Data Exchange (ETDEWEB)

Ken Thomas; Ted Quinn; Jerry Mauck; Richard Bockhorst

2014-09-01

There are significant developments underway in new types of actuators for power plant active components. Many of these make use of digital technology to provide a wide array of benefits in performance of the actuators and in reduced burden to maintain them. These new product offerings have gained considerable acceptance in use in process plants. In addition, they have been used in conventional power generation very successfully. This technology has been proven to deliver the benefits promised and substantiate the claims of improved performance. The nuclear industry has been reluctant to incorporate digital actuator technology into nuclear plant designs due to concerns due to a number of concerns. These could be summarized as cost, regulatory uncertainty, and a certain comfort factor with legacy analog technology. The replacement opportunity for these types of components represents a decision point for whether to invest in more modern technology that would provide superior operational and maintenance benefits. Yet, the application of digital technology has been problematic for the nuclear industry, due to qualification and regulatory issues. With some notable exceptions, the result has been a continuing reluctance to undertake the risks and uncertainties of implementing digital actuator technology when replacement opportunities present themselves. Rather, utilities would typically prefer to accept the performance limitations of the legacy analog actuator technologies to avoid impacts to project costs and schedules. The purpose of this report is to demonstrate that the benefits of digital actuator technology can be significant in terms of plant performance and that it is worthwhile to address the barriers currently holding back the widespread development and use of this technology. It addresses two important objectives in pursuit of the beneficial use of digital actuator technology for nuclear power plants: 1. To demonstrate the benefits of digital actuator

Technology as a Time Challenge: Study, Concept and Types of Technology

Directory of Open Access Journals (Sweden)

Vadim M. Rozin

2017-09-01

Full Text Available In the article divorce the concepts of engineering and technology. Designated four stages of technological development: the fi rst phase — “pilot technique”, it is characterized by magical conceptualization, the second — engineering (rational conceptualization, third design, fourth technology. For technical equipment is characterized by four features: technology is the artifacts, the technique can be considered as a “social body” of a person or society, technology is a useful way to use the forces of nature, and fi nally, the mediation in the form of tools, machines, and material environment that allows you to implement the ideas of man. It is argued that the conceptualization of technology is the essential characteristic of the concept. If you write, for example, about the technology of the Neolithic age or era of construction of the Egyptian pyramids, then we are talking about retrospective interpretation, from the point of view of modern understanding of technology. It’s not useless, for example, to determine preconditions of formation technology, but in terms of thinking creates problems and contradictions. The author argues that technology develops in the second half of the eighteenth century as a new reality, which describes the industrial activities in language operations and their conditions of division of labor and management. At the same time, technology is being characterized by the installation of quality, savings, standardization, and rational description of the production processes, their optimization for the training of new technologists. Discusses three stages of development of the technology and features of the main types of technology: production technology, engineering, large techno-social projects, global technology. Considering the author and the conditions of the development of new technologies. These include “technological zone of proximal development”, as well as two situation — relevant issues and

Decontamination and decommissioning technology tree and the current status of the technologies

Energy Technology Data Exchange (ETDEWEB)

Oh, Won Zin; Won, H.J.; Kim, G.N.; Lee, K.W.; Chol, W.K.; Jung, C.H.; Kim, C.J.; Kim, S.H.; Kwon, S.O.; Chung, C.M

2001-03-01

A technology tree diagram was developed on the basis of the necessary technologies applicable to the decontamination and decommissioning of nuclear facilities. The technology tree diagram is consist of 6 main areas such as characterization, decontamination, decommissioning and remote technology, radwaste management, site restoration, and decommissioning plan and engineering. Characterization is divided into 4 regions such as sampling and data collection, general characterization, chemical analysis and radiological analysis. Decontamination is also divided into 4 regions such as chemical decontamination, mechanical decontamination, the other decontamination technologies and new decontamination technologies. Decommissioning and remote technology area is divided into 4 regions such as cutting techniques, decommissioning technologies, new developing technologies and remote technologies. Radwaste management area is divided into 5 regions such as solid waste treatment, sludge treatment, liquid waste treatment, gas waste treatment and thermal treatment. Site restoration area is divided into 3 regions such as the evaluation of site contamination, soil decontamination and ground water decontamination. Finally, permission, decommissioning process, cost evaluation, quality assurance and the estimation of radionuclide inventory were mentioned in the decommissioning plan and engineering area. The estimated items for each technology are applicable domestic D and D facilities, D and D problem area and contamination/requirement, classification of D and D technology, similar technology, principle and overview of technology, status, science technology needs, implementation needs, reference and contact point.

Decontamination and decommissioning technology tree and the current status of the technologies

International Nuclear Information System (INIS)

Oh, Won Zin; Won, H. J.; Kim, G. N.; Lee, K. W.; Chol, W. K.; Jung, C. H.; Kim, C. J.; Kim, S. H.; Kwon, S. O.; Chung, C. M.

2001-03-01

A technology tree diagram was developed on the basis of the necessary technologies applicable to the decontamination and decommissioning of nuclear facilities. The technology tree diagram is consist of 6 main areas such as characterization, decontamination, decommissioning and remote technology, radwaste management, site restoration, and decommissioning plan and engineering. Characterization is divided into 4 regions such as sampling and data collection, general characterization, chemical analysis and radiological analysis. Decontamination is also divided into 4 regions such as chemical decontamination, mechanical decontamination, the other decontamination technologies and new decontamination technologies. Decommissioning and remote technology area is divided into 4 regions such as cutting techniques, decommissioning technologies, new developing technologies and remote technologies. Radwaste management area is divided into 5 regions such as solid waste treatment, sludge treatment, liquid waste treatment, gas waste treatment and thermal treatment. Site restoration area is divided into 3 regions such as the evaluation of site contamination, soil decontamination and ground water decontamination. Finally, permission, decommissioning process, cost evaluation, quality assurance and the estimation of radionuclide inventory were mentioned in the decommissioning plan and engineering area. The estimated items for each technology are applicable domestic D and D facilities, D and D problem area and contamination/requirement, classification of D and D technology, similar technology, principle and overview of technology, status, science technology needs, implementation needs, reference and contact point

Technological Determinism in Educational Technology Research: Some Alternative Ways of Thinking about the Relationship between Learning and Technology

Science.gov (United States)

Oliver, M.

2011-01-01

This paper argues that research on the educational uses of technology frequently overemphasizes the influence of technology. Research in the field is considered a form of critical perspective, and assumptions about technology are questioned. Technological determinism is introduced, and different positions on this concept are identified. These are…

SUSTAINABILITY LOGISTICS BASING SCIENCE AND TECHNOLOGY OBJECTIVE DEMONSTRATION; SELECTED TECHNOLOGY ASSESSMENT

Science.gov (United States)

2018-03-22

BASING SCIENCE AND TECHNOLOGY OBJECTIVE – DEMONSTRATION; SELECTED TECHNOLOGY ASSESSMENT by Gregg J. Gildea Paul D. Carpenter Benjamin J...Campbell William F. Harris* Michael A. McCluskey** and José A. Miletti*** *General Dynamics Information Technology Fairfax, VA 22030 **Maneuver...SCIENCE AND TECHNOLOGY OBJECTIVE – DEMONSTRATION; SELECTED TECHNOLOGY ASSESSMENT 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT

EPA'S ENVIRONMENTAL TECHNOLOGIES

Science.gov (United States)

The use of innovative technology is impeded by the lack of independent, credible information as to how the technology performs. Such data is needed by technology buyers and regulatory decision makers to make informed decisions on technologies that represent good financial invest...

Cooperative technology development: An approach to advancing energy technology

International Nuclear Information System (INIS)

Stern, T.

1989-09-01

Technology development requires an enormous financial investment over a long period of time. Scarce national and corporate resources, the result of highly competitive markets, decreased profit margins, wide currency fluctuations, and growing debt, often preclude continuous development of energy technology by single entities, i.e., corporations, institutions, or nations. Although the energy needs of the developed world are generally being met by existing institutions, it is becoming increasingly clear that existing capital formation and technology transfer structures have failed to aid developing nations in meeting their growing electricity needs. This paper will describe a method for meeting the electricity needs of the developing world through technology transfer and international cooperative technology development. The role of nuclear power and the advanced passive plant design will be discussed. (author)

Technology and Nursing

OpenAIRE

Maria Vera Lúcia Moreira Leitão Cardoso

2012-01-01

In the present age we cannot disassociate from emerging issues, which involve science, communication, health and technology, the influence of media, technological advances, and the use of computers in all spheres of life. The concepts created for technology cover various evaluation approaches, which depend upon which type of technology, approaches, usefulness and influences in a particular area of knowledge. Technological advances cover several areas, figuring quantum physics, nanotechnology,...

Modeling technological learning and its application for clean coal technologies in Japan

International Nuclear Information System (INIS)

Nakata, Toshihiko; Sato, Takemi; Wang, Hao; Kusunoki, Tomoya; Furubayashi, Takaaki

2011-01-01

Estimating technological progress of emerging technologies such as renewables and clean coal technologies becomes important for designing low carbon energy systems in future and drawing effective energy policies. Learning curve is an analytical approach for describing the decline rate of cost and production caused by technological progress as well as learning. In the study, a bottom-up energy-economic model including an endogenous technological learning function has been designed. The model deals with technological learning in energy conversion technologies and its spillover effect. It is applied as a feasibility study of clean coal technologies such as IGCC (Integrated Coal Gasification Combined Cycle) and IGFC (Integrated Coal Gasification Fuel Cell System) in Japan. As the results of analysis, it is found that technological progress by learning has a positive impact on the penetration of clean coal technologies in the electricity market, and the learning model has a potential for assessing upcoming technologies in future.

Sustainability of University Technology Transfer: Mediating Effect of Inventor’s Technology Service

Directory of Open Access Journals (Sweden)

Fang Li

2018-06-01

Full Text Available Based on the perspective of knowledge transfer and the technology acceptance model (TAM, this paper constructs a university technology transfer sustainable development model that considers the inventor’s technology service from the perspective of the long-term cooperation of enterprise, and analyzes the mediating effect of the inventor’s technology service on university technology transfer sustainability. By using 270 questionnaires as survey data, it is found that the availability of an inventor’s technology service has a significant positive impact on the attitude tendency and practice tendency of enterprise long-term technological cooperation; enterprise technology absorption capacity and trust between a university and an enterprise also have significant influence on an inventor’s technical service availability. Therefore, the inventor’s technology service acts as a mediator in the relationship between university technology transfer sustainability and influence factors. Universities ought to establish the technology transfer model, which focuses on the inventor’s tacit knowledge transfer service, and promotes the sustainable development of the university.

Technology '90

Energy Technology Data Exchange (ETDEWEB)

1991-01-01

The US Department of Energy (DOE) laboratories have a long history of excellence in performing research and development in a number of areas, including the basic sciences, applied-energy technology, and weapons-related technology. Although technology transfer has always been an element of DOE and laboratory activities, it has received increasing emphasis in recent years as US industrial competitiveness has eroded and efforts have increased to better utilize the research and development resources the laboratories provide. This document, Technology '90, is the latest in a series that is intended to communicate some of the many opportunities available for US industry and universities to work with the DOE and its laboratories in the vital activity of improving technology transfer to meet national needs. Technology '90 is divided into three sections: Overview, Technologies, and Laboratories. The Overview section describes the activities and accomplishments of the DOE research and development program offices. The Technologies section provides descriptions of new technologies developed at the DOE laboratories. The Laboratories section presents information on the missions, programs, and facilities of each laboratory, along with a name and telephone number of a technology transfer contact for additional information. Separate papers were prepared for appropriate sections of this report.

FY-95 technology catalog. Technology development for buried waste remediation

International Nuclear Information System (INIS)

1995-01-01

The US Department of Energy's (DOE) Buried Waste Integrated Demonstration (BWID) program, which is now part of the Landfill Stabilization Focus Area (LSFA), supports applied research, development, demonstration, and evaluation of a multitude of advanced technologies dealing with underground radioactive and hazardous waste remediation. These innovative technologies are being developed as part of integrated comprehensive remediation systems for the effective and efficient remediation of buried waste sites throughout the DOE complex. These efforts are identified and coordinated in support of Environmental Restoration (EM-40) and Waste Management (EM-30) needs and objectives. Sponsored by the DOE Office of Technology Development (EM-50), BWID and LSFA work with universities and private industry to develop technologies that are being transferred to the private sector for use nationally and internationally. This report contains the details of the purpose, logic, and methodology used to develop and demonstrate DOE buried waste remediation technologies. It also provides a catalog of technologies and capabilities with development status for potential users. Past FY-92 through FY-94 technology testing, field trials, and demonstrations are summarized. Continuing and new FY-95 technology demonstrations also are described

FY-95 technology catalog. Technology development for buried waste remediation

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-10-01

The US Department of Energy`s (DOE) Buried Waste Integrated Demonstration (BWID) program, which is now part of the Landfill Stabilization Focus Area (LSFA), supports applied research, development, demonstration, and evaluation of a multitude of advanced technologies dealing with underground radioactive and hazardous waste remediation. These innovative technologies are being developed as part of integrated comprehensive remediation systems for the effective and efficient remediation of buried waste sites throughout the DOE complex. These efforts are identified and coordinated in support of Environmental Restoration (EM-40) and Waste Management (EM-30) needs and objectives. Sponsored by the DOE Office of Technology Development (EM-50), BWID and LSFA work with universities and private industry to develop technologies that are being transferred to the private sector for use nationally and internationally. This report contains the details of the purpose, logic, and methodology used to develop and demonstrate DOE buried waste remediation technologies. It also provides a catalog of technologies and capabilities with development status for potential users. Past FY-92 through FY-94 technology testing, field trials, and demonstrations are summarized. Continuing and new FY-95 technology demonstrations also are described.

Technology cycles and technology revolutions

Energy Technology Data Exchange (ETDEWEB)

Paganetto, Luigi; Scandizzo, Pasquale Lucio

2010-09-15

Technological cycles have been characterized as the basis of long and continuous periods economic growth through sustained changes in total factor productivity. While this hypothesis is in part consistent with several theories of growth, the sheer magnitude and length of the economic revolutions experienced by humankind seems to indicate surmise that more attention should be given to the origin of major technological and economic changes, with reference to one crucial question: role of production and use of energy in economic development.

Mars Technology Program: Planetary Protection Technology Development

Science.gov (United States)

Lin, Ying

2006-01-01

This slide presentation reviews the development of Planetary Protection Technology in the Mars Technology Program. The goal of the program is to develop technologies that will enable NASA to build, launch, and operate a mission that has subsystems with different Planetary Protection (PP) classifications, specifically for operating a Category IVb-equivalent subsystem from a Category IVa platform. The IVa category of planetary protection requires bioburden reduction (i.e., no sterilization is required) The IVb category in addition to IVa requirements: (i.e., terminal sterilization of spacecraft is required). The differences between the categories are further reviewed.

This damned technology

International Nuclear Information System (INIS)

Steinbuch, K.

1982-01-01

Technology is not an end in itself, but a means to an end for human existence. If it does not serve this purpose it must be adapted or changed. Criticism of technology is necessary in view of the various positive and negative consequences of technology, it must however be based on judgement and sense of responsibility. It is however often claimed that technology and industry are the tools of the evil and that the technical intelligentsia do not feel responsible for psychological, social and political consequences of their doing. By contributions of several authors and of different periods this book elucidates the often grotesque contradictions between demagogic accusation of technology and the reality of technology; this book presents principal considerations on an acceptable assessment of technology. (HSCH) [de

Technology-Use Mediation

DEFF Research Database (Denmark)

Bansler, Jørgen P.; Havn, Erling C.

2003-01-01

This study analyzes how a group of ‘mediators’ in a large, multinational company adapted a computer-mediated communication technology (a ‘virtual workspace’) to the organizational context (and vice versa) by modifying features of the technology, providing ongoing support for users, and promoting...... appropriate conventions of use. Our findings corroborate earlier research on technology-use mediation, which suggests that such mediators can exert considerable influence on how a particular technology will be established and used in an organization. However, this study also indicates that the process...... of technology-use mediation is more complex and indeterminate than earlier literature suggests. In particular, we want to draw attention to the fact that advanced computer-mediated communication technologies are equivocal and that technology-use mediation consequently requires ongoing sensemaking (Weick 1995)....

Key technologies book

International Nuclear Information System (INIS)

1997-01-01

In this book can be found all the useful information on the French industry key technologies of the years 2000-2005. 136 technologies at the junction of the science advances and of the markets expectations are divided into 9 sectors. Among them, only 4 are interesting here: the environment, the transports, the materials and the energy. In 1995, the secretary's office of State for industry has published a first synthesis book on these key technologies. This 1997 new key technologies book extends and completes the initial study. For each key technology, an encyclopedic sheet is given. Each sheet combines thus some exact and practical information on: advance state of the technology, market characteristics, development forecasts, occupation and involved sectors, technology acquisition cost, research programs but also contacts of the main concerned efficiency poles. (O.M.)

Hackers against technology: Critique and recuperation in technological cycles.

Science.gov (United States)

Maxigas

2017-12-01

I offer an interpretation of hackers' technological choices through a theoretical framework of critique and recuperation in technological cycles, building on prior research that brings the pragmatic sociology of Boltanski and Chiapello to bear on matters in Science and Technology Studies. I argue that contextualizing technology choices in the development of capitalism through innovation illuminates their political significance. I start with the counterintuitive observation that some browser extensions popular with hackers, like RequestPolicy, make it considerably harder for them to look at websites. This observation showcases the Luddite aspects of hackerdom, in that they are willing to 'break' popular websites that would otherwise cheat on the user. In line with an undercurrent of hacker studies, in this case study I find hackers fighting technological progress they see as social decline.

Search Technologies | NCI Technology Transfer Center | TTC

Science.gov (United States)

Our team of technology transfer specialists has specialized training in invention reporting, patenting, patent strategy, executing technology transfer agreements and marketing. TTC is comprised of professionals with diverse legal, scientific, and business/marketing expertise. Most of our staff hold doctorate-level technical and/or legal training.

Available Technologies | NCI Technology Transfer Center | TTC

Science.gov (United States)

Our team of technology transfer specialists has specialized training in invention reporting, patenting, patent strategy, executing technology transfer agreements and marketing. TTC is comprised of professionals with diverse legal, scientific, and business/marketing expertise. Most of our staff hold doctorate-level technical and/or legal training.

On technology blending.

OpenAIRE

Rosenberg N

1986-01-01

ILO pub-WEP pub. Working paper on the blending of traditional technology and technological change in developing countries - argues that choice of technology should be compatible with labour intensive requirements and local level management and economic conditions; considers employment creation and economic implications; concludes that technology transfer should be selective. References.

Different Perspectives on Technology Acceptance: The Role of Technology Type and Age

Science.gov (United States)

Arning, Katrin; Ziefle, Martina

Although eHealth technologies offer an enormous potential to improve healthcare, the knowledge about key determinants of acceptance for eHealth technology is restricted. While the underlying technology of eHealth technologies and Information and Communication technology (ICT) is quite similar, utilization contexts and using motives are quite different. In order to explore the role of technology type on acceptance, we contrasted central application characteristics of both technology types using the scenario technique. A questionnaire was administered (n = 104) measuring individual variables (age, gender) and attitudes regarding an eHealth application (blood sugar meter) in contrast to an ICT device (Personal Digital Assistant, PDA). Older users basically approved the utilization of health-related technologies and perceived lower usability barriers. In addition, we identified main utilization motives of eHealth technology and technology-specific acceptance patterns, especially regarding issues of data safety in the eHealth context. Effects of age and gender in acceptance ratings suggest a differential perspective on eHealth acceptance. Finally, practical interventions were derived in order to support eHealth device design and to promote acceptance of eHealth technology.

Technology transfer and development: a preliminary look at Chinese technology in Guyana

Energy Technology Data Exchange (ETDEWEB)

Long, F

1982-05-01

Technology is regarded as a vital ingredient for development. Since developing countries can hardly fill their technological requirements indigenously, such countries tend to acquire the bulk of technology applied to their production systems from abroad. However, the transfer of technology tends to be associated with a series of problems: foreign exchange, inappropriateness, the generation of limited inter-sectorial linkages, limited use of raw materials, and other inputs associated with technology dependency. The study points to the fact that technology transfer need not necessarily be associated with the disadvantages identified in the literature. The study which essentially looks at the use of Chinese technology in clay-brick manufacturing in Guyana, shows that the country was able to reap several development benefits from the technology-transfer arrangement. At the same time, certain problems arising from the technology-transfer package such as the transfer of critical skills in key areas of production, and maintenance and servicing, are discussed. But these, the author argues, are not a function of restrictive conditions found in technology-transfer clauses, but rather of improper technology-transfer management. 2 tables.

Technological risks

International Nuclear Information System (INIS)

Klinke, A.; Renn, O.

1998-01-01

The empirical part about the technological risks deals with different technologies: nuclear energy, early warning systems of nuclear weapons and NBC-weapons, and electromagnetic fields. The potential of damage, the contemporary management strategies and the relevant characteristics will be described for each technology: risks of nuclear energy; risks of early warning systems of nuclear weapons and NBC-weapons; risks of electromagnetic fields. (authors)

Technological risks

Energy Technology Data Exchange (ETDEWEB)

Klinke, A.; Renn, O. [Center of Technology Assessment in Baden-Wuerttemberg, Stuttgart (Germany)

1998-07-01

The empirical part about the technological risks deals with different technologies: nuclear energy, early warning systems of nuclear weapons and NBC-weapons, and electromagnetic fields. The potential of damage, the contemporary management strategies and the relevant characteristics will be described for each technology: risks of nuclear energy; risks of early warning systems of nuclear weapons and NBC-weapons; risks of electromagnetic fields. (authors)

Inter-technology knowledge spillovers for energy technologies

International Nuclear Information System (INIS)

Nemet, Gregory F.

2012-01-01

Both anecdotal evidence and the innovation literature indicate that important advances in energy technology have made use of knowledge originating in other technological areas. This study uses the set of U.S. patents granted from 1976 to 2006 to assess the role of knowledge acquired from outside each energy patent's technological classification. It identifies the effect of external knowledge on the forward citation frequency of energy patents. The results support the claim above. Regression coefficients on citations to external prior art are positive and significant. Further, the effect of external citations is significantly larger than that of other types of citations. Conversely, citations to prior art that is technologically near have a negative effect on forward citation frequency. These results are robust across several alternative specifications and definitions of whether each flow of knowledge is external. Important energy patents have drawn heavily from external prior art categorized as chemical, electronics, and electrical; they cite very little prior art from computers, communications, and medical inventions.

Technology in the Montessori Classroom: Teachers' Beliefs and Technology Use

Science.gov (United States)

Jones, Sara J.

2017-01-01

As technology becomes ubiquitous in society, there is increasing momentum to incorporate it into education. Montessori education is not immune to this push for technology integration. This qualitative study investigates four Upper Elementary Montessori teachers' attitudes toward technology and technology integration in a public school setting.…

2017 Technology Showcase | NCI Technology Transfer Center | TTC

Science.gov (United States)

The 2017 Technology Showcase is an inaugural, half-day event showcased technologies developed by the National Cancer Institute's Center for Cancer Research (CCR) and the Frederick National Laboratory for Cancer Research (FNLCR).

Technology in geriatrics.

Science.gov (United States)

Pilotto, Alberto; Boi, Raffaella; Petermans, Jean

2018-03-13

Recently, the interest of industry, government agencies and healthcare professionals in technology for aging people has increased. The challenge is whether technology may play a role in enhancing independence and quality of life and in reducing individual and societal costs of caring. Information and communication technologies, i.e. tools aimed at communicating and informing, assistive technologies designed to maintain older peoples' independence and increasing safety, and human-computer interaction technologies for supporting older people with motility and cognitive impairments as humanoid robots, exoskeletons, rehabilitation robots, service robots and companion-type are interdisciplinary topics both in research and in clinical practice. The most promising clinical applications of technologies are housing and safety to guarantee older people remaining in their own homes and communities, mobility and rehabilitation to improve mobility and gait and communication and quality of life by reducing isolation, improve management of medications and transportation. Many factors impair a broad use of technology in older age, including psychosocial and ethical issues, costs and fear of losing human interaction. A substantial lack of appropriate clinical trials to establish the clinical role of technologies to improve physical or cognitive performances and/or quality of life of subjects and their caregivers may suggest that the classical biomedical research model may not be the optimal choice to evaluate technologies in older people. In conclusion, successful technology development requires a great effort in interdisciplinary collaboration to integrate technologies into the existing health and social service systems with the aim to fit into the older adults' everyday life.

GENERAL ENVIRONMENTAL CORPORATION; CURE ELECTROCOAGULATION TECHNOLOGY: INNOVATIVE TECHNOLOGY EVALUATION REPORT

Science.gov (United States)

The CURE electrocoagulation technology was demonstrated under the Superfund Innovative Technology Evaluation (SITE) program at the U.S. Department of Energy (DOE) Rocky Flats Environmental Technology Site (RFETS), where water from the solar evaporation ponds (SEPs) was contaminat...

Technology transfer and commercialization of in situ vitrification technology

International Nuclear Information System (INIS)

Williams, L.D.; Hansen, J.E.

1992-01-01

In situ vitrification (ISV) technology was conceived and an initial proof-of-principle test was conducted in 1980 by Battelle Memorial Institute for the U.S. Department of Energy (DOE) at Pacific Northwest Laboratory (PNL). The technology was rapidly developed through bench, engineering pilot, and large scales in the following years. In 1986, DOE granted rights to the basic ISV patent to Battelle in exchange for a commitment to commercialize the technology. Geosafe Corporation was established as the operating entity to accomplish the commercialization objective. This paper describes and provides status information on the technology transfer and commercialization effort

When technology, science and culture meet: insights from ancient Chinese technology

Science.gov (United States)

Lee, Yeung Chung

2017-10-01

This paper draws together two important agendas in science education. The first is making science education more inclusive such that students from non-Western or indigenous cultures can benefit from culturally relevant curricula. The second is integrating technology into the curriculum under the umbrella of Science-Technology-Society (STS) education to embrace the social aspects of science, with technology serving as a bridge. The advancement of the first agenda is hindered by the pursuance by both Western and non-Western societies of narrow cultural and practical goals without considering the development of science and technology from a cross-cultural perspective. The second agenda is limited by the misconception that technology is applied science, leading to the exclusion from STS discussions of pre-science or indigenous technologies developed by non-Western cultures. Through selected case studies of the evolution of Chinese traditional technologies and their interaction with science, this paper offers a perspective from the Far East, and argues for situating culturally responsive science education in broader historical and cross-cultural contexts to acknowledge the multi-cultural contributions to science and technology. A form of cross-cultural STS education is advanced, encompassing the cultural basis of technological developments, technology diffusion, interactions of traditional technology with science, and the potential development of traditional or indigenous technologies. This approach provides a bridge between the existing universal science education paradigm promoted in the West and the different forms of multi-cultural education advocated by indigenous science educators. To translate theory into practice, a conceptual framework is proposed in which the essential transdisciplinary knowledge base, curricular goals, and pedagogical approaches are embedded.

The Human Technology

DEFF Research Database (Denmark)

Fausing, Bent

 Bent Fausing  "The Humane Technology", abstract (for The Two Cultures: Balancing Choices and Effects Oxford University July 20-26, 2008). The paper will investigate the use of technology in everyday aesthetics such as TV-commercials for mobile phones for Nokia, which slogan is, as it is well known......, "Nokia - connecting people". Which function does this technology get in narratives, images, interactions and affects here?      The mobile phone and its digital camera are depicted as being able to make a unique human presence and interaction. The medium, the technology is a necessary helper to get...... towards this very special and lost humanity. Without the technology, no special humanity is the prophecy. This personification or anthropomorphism is important for the branding of new technology. The technology is seen as creating a technotranscendens towards a more qualified humanity, which is in contact...

Physics and high technology

International Nuclear Information System (INIS)

Shao Liqin; Ma Junru.

1992-01-01

At present, the development of high technology has opened a new chapter in world's history of science and technology. This review describes the great impact of physics on high technology in six different fields (energy technology, new materials, information technology, biotechnology, space technology, and Ocean technology). It is shown that the new concepts and new methods created in physics and the special conditions and measurements established for physics researches not only deepen human's knowledge about nature but also point out new directions for engineering and technology. The achievements in physics have been more and more applied to high technology, while the development of high technology has explored some new research areas and raised many novel, important projects for physics. Therefore, it is important for us to strengthen the research on these major problems in physics

Advanced manufacturing: Technology diffusion

Energy Technology Data Exchange (ETDEWEB)

Tesar, A.

1995-12-01

In this paper we examine how manufacturing technology diffuses rom the developers of technology across national borders to those who do not have the capability or resources to develop advanced technology on their own. None of the wide variety of technology diffusion mechanisms discussed in this paper are new, yet the opportunities to apply these mechanisms are growing. A dramatic increase in technology diffusion occurred over the last decade. The two major trends which probably drive this increase are a worldwide inclination towards ``freer`` markets and diminishing isolation. Technology is most rapidly diffusing from the US In fact, the US is supplying technology for the rest of the world. The value of the technology supplied by the US more than doubled from 1985 to 1992 (see the Introduction for details). History shows us that technology diffusion is inevitable. It is the rates at which technologies diffuse to other countries which can vary considerably. Manufacturers in these countries are increasingly able to absorb technology. Their manufacturing efficiency is expected to progress as technology becomes increasingly available and utilized.

The Western Environmental Technology Office (WETO), Butte, Montana, technology summary

International Nuclear Information System (INIS)

1994-09-01

This document has been prepared by the DOE Environmental Management (EM) Office of Technology Development (OTD) to highlight its research, development, demonstration, testing, and evaluation activities funded through the Western Environmental Technology Office (WETO) in Butte, Montana. Technologies and processes described have the potential to enhance DOE's cleanup and waste management efforts, as well as improve US industry's competitiveness in global environmental markets. WETO's environmental technology research and testing activities focus on the recovery of useable resources from waste. Environmental technology development and commercialization activities will focus on mine cleanup, waste treatment, resource recovery, and water resource management. Since the site has no record of radioactive material use and no history of environmental contamination/remediation activities, DOE-EM can concentrate on performing developmental and demonstration activities without the demands of regulatory requirements and schedules. Thus, WETO will serve as a national resource for the development of new and innovative environmental technologies

Electron Beam Technology and Other Irradiation Technology Applications in the Food Industry.

Science.gov (United States)

Pillai, Suresh D; Shayanfar, Shima

2017-02-01

Food irradiation is over 100 years old, with the original patent for X-ray treatment of foods being issued in early 1905, 20 years after there discovery by W. C. Roentgen in 1885. Since then, food irradiation technology has become one of the most extensively studied food processing technologies in the history of mankind. Unfortunately, it is the one of the most misunderstood technologies with the result that there are rampant misunderstandings of the core technology, the ideal applications, and how to use it effectively to derive the maximum benefits. There are a number of books, book chapters, and review articles that provide overviews of this technology [25, 32, 36, 39]. Over the last decade or so, the technology has come into greater focus because many of the other pathogen intervention technologies have been unable to provide sustainable solutions on how to address pathogen contamination in foods. The uniqueness of food irradiation is that this technology is a non-thermal food processing technology, which unto itself is a clear high-value differentiator from other competing technologies.

Emerging Technologies Integrating Technology into Study Abroad

Science.gov (United States)

Godwin-Jones, Robert

2016-01-01

"Ready access to travel and to technology-enhanced social networking (e.g., Facebook or Skype) has changed the nature of study abroad to the point where today's experiences are fundamentally different from those of earlier eras" (Kinginger, 2013a, p. 345). In addition to more travel options and greater technology availability, study…

Army Technology

Science.gov (United States)

2015-02-01

that allows them to perform applied research under the Institute for Biotechnology research team 1 2 3 20 | ARMY TECHNOLOGY MAGAZINE ...DASA(R&T) Deputy Assistant Secretary of the Army for Research and Technology Download the magazine , view online or read each individual story with...Army photo by Conrad Johnson) Front and back cover designs by Joe Stephens EXECUTIVE DEPUTY TO THE COMMANDING GENERAL Army Technology Magazine is an

Characterization, monitoring, and sensor technology crosscutting program: Technology summary

International Nuclear Information System (INIS)

1995-06-01

The purpose of the Characterization, Monitoring, and Sensor Technology Crosscutting Program (CMST-CP) is to deliver appropriate characterization, monitoring, and sensor technology (CMST) to the Office of Waste Management (EM-30), the Office of Environmental Restoration (EM-40), and the Office of Facility Transition and Management (EM-60). The technology development must also be cost effective and appropriate to EM-30/40/60 needs. Furthermore, the required technologies must be delivered and implemented when needed. Accordingly, and to ensure that available DOE and other national resources are focused an the most pressing needs, management of the technology development is concentrated on the following Focus Areas: Contaminant Plume Containment and Remediation (PFA); Landfill Stabilization (LSFA); High-Level Waste Tank Remediation (TFA); Mixed Waste Characterization, Treatment, and Disposal (MWFA); and Facility Deactivation, Decommissioning, and Material Disposition (FDDMDFA). Brief descriptions of CMST-CP projects funded in FY95 are presented

Characterization, monitoring, and sensor technology crosscutting program: Technology summary

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-06-01

The purpose of the Characterization, Monitoring, and Sensor Technology Crosscutting Program (CMST-CP) is to deliver appropriate characterization, monitoring, and sensor technology (CMST) to the Office of Waste Management (EM-30), the Office of Environmental Restoration (EM-40), and the Office of Facility Transition and Management (EM-60). The technology development must also be cost effective and appropriate to EM-30/40/60 needs. Furthermore, the required technologies must be delivered and implemented when needed. Accordingly, and to ensure that available DOE and other national resources are focused an the most pressing needs, management of the technology development is concentrated on the following Focus Areas: Contaminant Plume Containment and Remediation (PFA); Landfill Stabilization (LSFA); High-Level Waste Tank Remediation (TFA); Mixed Waste Characterization, Treatment, and Disposal (MWFA); and Facility Deactivation, Decommissioning, and Material Disposition (FDDMDFA). Brief descriptions of CMST-CP projects funded in FY95 are presented.

Advanced Communication Technology Satellite (ACTS) multibeam antenna technology verification experiments

Science.gov (United States)

Acosta, Roberto J.; Larko, Jeffrey M.; Lagin, Alan R.

1992-01-01

The Advanced Communication Technology Satellite (ACTS) is a key to reaching NASA's goal of developing high-risk, advanced communications technology using multiple frequency bands to support the nation's future communication needs. Using the multiple, dynamic hopping spot beams, and advanced on board switching and processing systems, ACTS will open a new era in communications satellite technology. One of the key technologies to be validated as part of the ACTS program is the multibeam antenna with rapidly reconfigurable hopping and fixed spot beam to serve users equipped with small-aperature terminals within the coverage areas. The proposed antenna technology experiments are designed to evaluate in-orbit ACTS multibeam antenna performance (radiation pattern, gain, cross pol levels, etc.).

Institutionalized Technological Foresight

DEFF Research Database (Denmark)

Koch, Christian; Hansen, Hans Henrik; Stissing Jensen, Jens

2008-01-01

, and they become dependent of national and other institutional foresights. Since 2000 the Danish ministry of Science, Technology and Innovation has tendered nine technology foresights. These could be used by SMEs as supplementary to internal decision making. One also expects to see these foresights placed firmly...... in the national strategy of coordinating and strengthening policy on research, technology and innovation in one superministry. The paper evaluates the methods, impact and role in policy making of these technological foresights. The particular role of institutionalized public foresight in relation to enterprise......Technology and knowledge monitoring is a continual challenge especially for small and medium size enterprises. Technological foresight seemingly offers important parts of this crucial monitoring. The SMEs rarely possess sufficient resources to do systematic foresights, or forecasts however...

Rover Technologies

Data.gov (United States)

National Aeronautics and Space Administration — Develop and mature rover technologies supporting robotic exploration including rover design, controlling rovers over time delay and for exploring . Technology...

ENVIRONMENTAL TECHNOLOGY VERIFICATION (ETV) PROGRAM: GREEN BUILDING TECHNOLOGIES

Science.gov (United States)

The U.S. Environmental Protection Agency (EPA) Environmental Technology Verification (ETV) Program evaluates the performance of innovative air, water, pollution prevention and monitoring technologies that have the potential to improve human health and the environment. This techno...

NASA Technology Transfer System

Science.gov (United States)

Tran, Peter B.; Okimura, Takeshi

2017-01-01

NTTS is the IT infrastructure for the Agency's Technology Transfer (T2) program containing 60,000+ technology portfolio supporting all ten NASA field centers and HQ. It is the enterprise IT system for facilitating the Agency's technology transfer process, which includes reporting of new technologies (e.g., technology invention disclosures NF1679), protecting intellectual properties (e.g., patents), and commercializing technologies through various technology licenses, software releases, spinoffs, and success stories using custom built workflow, reporting, data consolidation, integration, and search engines.

LIEKKI 2 - Combustion technology is environmental technology

Energy Technology Data Exchange (ETDEWEB)

Hupa, M. [Aabo Akademi, Turku (Finland)

1996-12-31

Finland has wide experience in applications of various combustion technologies and fuels and in supplying energy to industry and municipalities. Furthermore, combustion hardware and equipment are amongst our most important export products. Above all, fluidized bed boilers, recovery boilers for pulp mills and heavy diesel engines and diesel power plants have achieved excellent success in the world markets. Exports of these products alone have amounted to several billions of Finnish marks of annual sales in recent years. Within modern combustion technology, the objective is to control flue gas emissions as far as possible in the process itself, thus doing away with the need for the separate scrubbing of flue gases. To accomplish this it has been necessary to conduct a large amount of research on the details of the chemistry of combustion emissions and the flows in furnaces and engine cylinders. A host of completely new products are being developed for the combustion technology field. The LIEKKI programme has been particularly interested in so-called combined-cycle processes based on pressurized fluidized bed technology

Technology Strategy for 'Environmental Technology for the Future'; Technology Target Areas; TTA1 - environmental technology for the future

Energy Technology Data Exchange (ETDEWEB)

2007-07-01

The OG21 Technology Target Area 1 (TTA 1) group has produced a strategy for 'Environmental Technology for the Future'. A key aim of this work is to ensure that the operators on the Norwegian Continental Shelf (NCS) remain in a leading position with respect to environmental performance, while contributing to optimised resource recovery and value creation. This strategy focuses on environmental technology, which includes hardware, methods, software and knowledge. The TTA 1 group has agreed on a common vision: 'Norwegian oil and gas activities shall be leading in environmental performance, and Norway shall have the world leading knowledge and technology cluster within environmental technologies to support the zero harmful impact goals of the oil and gas industry.' Priorities have been made with emphasis on gaps that are considered most important to close and that will benefit from public research and development funding either for initialisation (primarily via the Petromaks and Climit programs) or acceleration (via Petromaks / Climit and particularly Demo 2000 where demonstration or piloting is required). The priorities aim to avoid technology gaps that are expected to be closed adequately through existing projects / programs or which are covered in other TTA strategies. The priority areas as identified are: Environmental impact and risk identification / quantification for new areas: Make quality assured environmental baseline data available on the web. Develop competence necessary to quantify and monitor the risks and risk reductions to the marine environment in new area ecosystems; Carbon capture and storage: Quantify environmental risks and waste management issues associated with bi-products from carbon capture processes and storage solutions. Develop and demonstrate effective carbon storage risk management, monitoring and mitigation technologies. Develop more cost and energy efficient power-from-shore solutions to reduce / eliminate CO{sub 2

Technological Knowledge and Reasoning in Finnish and Estonian Technology Education

Science.gov (United States)

Autio, Ossi; Soobik, Mart

2017-01-01

The main idea of this research was to find out if there is a relationship between students' undertakings within Craft and Technology education and their ability to understand technological concepts. Study participants' technological knowledge and reasoning was measured with a questionnaire regarding mechanical systems connected with simple…

The Western Environmental Technology Office (WETO), Butte, Montana, technology summary

Energy Technology Data Exchange (ETDEWEB)

1994-09-01

This document has been prepared by the DOE Environmental Management (EM) Office of Technology Development (OTD) to highlight its research, development, demonstration, testing, and evaluation activities funded through the Western Environmental Technology Office (WETO) in Butte, Montana. Technologies and processes described have the potential to enhance DOE`s cleanup and waste management efforts, as well as improve US industry`s competitiveness in global environmental markets. WETO`s environmental technology research and testing activities focus on the recovery of useable resources from waste. Environmental technology development and commercialization activities will focus on mine cleanup, waste treatment, resource recovery, and water resource management. Since the site has no record of radioactive material use and no history of environmental contamination/remediation activities, DOE-EM can concentrate on performing developmental and demonstration activities without the demands of regulatory requirements and schedules. Thus, WETO will serve as a national resource for the development of new and innovative environmental technologies.

Magnetic fusion technology

CERN Document Server

Dolan, Thomas J

2014-01-01

Magnetic Fusion Technology describes the technologies that are required for successful development of nuclear fusion power plants using strong magnetic fields. These technologies include: ? magnet systems, ? plasma heating systems, ? control systems, ? energy conversion systems, ? advanced materials development, ? vacuum systems, ? cryogenic systems, ? plasma diagnostics, ? safety systems, and ? power plant design studies. Magnetic Fusion Technology will be useful to students and to specialists working in energy research.

New Mobile Technologies

DEFF Research Database (Denmark)

Tan, Su-En; Henten, Anders

2006-01-01

This paper takes a look at Clayton Christensen 's theory of disruptive technologies and how Christensen's theory relates to other innovation theories. It also proposes a new layer of analysis to this theory to better link the technology analysis to the market analysis of any given technology...... product. This layer suggests that complementarity and substitutability are important criteria for technologies to be market disruptions or sustaining changes....

[Earth Science Technology Office's Computational Technologies Project

Science.gov (United States)

Fischer, James (Technical Monitor); Merkey, Phillip

2005-01-01

This grant supported the effort to characterize the problem domain of the Earth Science Technology Office's Computational Technologies Project, to engage the Beowulf Cluster Computing Community as well as the High Performance Computing Research Community so that we can predict the applicability of said technologies to the scientific community represented by the CT project and formulate long term strategies to provide the computational resources necessary to attain the anticipated scientific objectives of the CT project. Specifically, the goal of the evaluation effort is to use the information gathered over the course of the Round-3 investigations to quantify the trends in scientific expectations, the algorithmic requirements and capabilities of high-performance computers to satisfy this anticipated need.

Technology in the Rear-View Mirror: How to Better Incorporate the History of Technology into Technology Education

Science.gov (United States)

Hallstrom, Jonas; Gyberg, Per

2011-01-01

The history of technology can play an important role in illuminating the fundamentals of technological change, but it is important that technology teachers, teacher educators, curriculum developers and researchers can be provided with good analytical tools for this purpose. In this article, we propose a model of techno-historical interplay, as a…

Discourses of Technology

DEFF Research Database (Denmark)

Sommer, Jannek K.; Knudsen, Gry Høngsmark

In this poster we address consumption of technology from the perspective of failure. A large body of studies of consumption of technology have focused on consumer acceptance (Kozinets, 2008). These studies have identified particular narratives about social and economic progress, and pleasure...... (Kozinets, 2008) as drivers of consumer acceptance of new technology. Similarly, Giesler (2008) has conceptualized consumer acceptance of technology as a form of marketplace drama, in which market ideologies are negotiated between consumers and media discourses. We suggest to study discourses around failed...... technology products to explore the negotiation of the familiar and alien that makes consumers reject or embrace a new technology. Thus, this particular project sets out to analyze consumer discourses surrounding the Google Glass video “How it Feels [through Google Glass]” on YouTube, because we want...

Technology transfer packages

International Nuclear Information System (INIS)

Mizon, G.A.; Bleasdale, P.A.

1994-01-01

Nuclear power is firmly established in many developed countries'energy policies and is being adopted by emerging nations as an attractive way of gaining energy self sufficiency. The early users of nuclear power had to develop the technology that they needed, which now, through increasing world wide experience, has been rationalised to meet demanding economic and environmental pressures. These justifiable pressures, can lead to existing suppliers of nuclear services to consider changing to more appropriate technologies and for new suppliers to consider licensing proven technology rather then incurring the cost of developing new alternatives. The transfer of technology, under license, is made more straight forward if the owner conveniently groups appropriate technology into packages. This paper gives examples of 'Technology Packages' and suggests criteria for the specification, selection and contractual requirements to ensure successful licensing

SITE TECHNOLOGY CAPSULE: DYNAPHORE, INC., FORAGER SPONGE TECHNOLOGY

Science.gov (United States)

The Forager Sponge is a volume reduction technology in which heavy metal contaminants from an aqueous medium are selectively concentrated into a smaller volume for facilitated disposal. he technology treats contaminated groundwater, surface waters and porous waters by absorbing d...

SITE TECHNOLOGY CAPSULE: DYNAPHORE, INC., FORAGER™ SPONGE TECHNOLOGY

Science.gov (United States)

The Forager™ Sponge is a volume reduction technology in which heavy metal contaminants from an aqueous medium are selectively concentrated into a smaller volume for facilitated disposal. The technology treats contaminated groundwater, surface waters, and process waters by absorbi...

Technological Style is History

DEFF Research Database (Denmark)

Blond, Lasse

The effort to comprehend innovation across cultures and time highlights the importance of the explicating factors external to technology. It becomes relevant to nuance or differentiate the understanding of social and cultural responses to adopted technologies by recognizing that technology shapes...... culture, and just as importantly that culture shapes technology. By looking at a recent transfer of technology this reciprocal exchange is elaborated by considering the cultural or contextual influence in the adaptation of technology. In this connection the notion of technological style is revisited...... by questioning whether it pays due attention to the non-technical factors of the process? In order to compensate for the deficiencies of the technological style as a sensitizing device the concept of sociotechnical style is introduced – a concept more in tune with resent research in technology studies....

Tailings technology. Decommissioning and rehabilitation remedial action technology development

International Nuclear Information System (INIS)

Ramsey, R.W. Jr.

1982-01-01

This paper is to provide an overview of technology requirements for long-term uranium mill tailings disposal and remedial actions for existing tailings to ensure their adequate disposal. The paper examines the scientific disciplines that are the basis for the technology of uranium mill tailings stabilization and the design of barriers to control radiological exposure or environmental degradation at the location of tailings disposal. The discussion is presented as a hypothetical course of instruction at a fictitious university. Features of six mechanisms of dispersal or intrusion are examined with brief discussion of the applicable technology development for each. The paper serves as an introduction to subsequent specific technology development papers in the session. (author)

User behavior and technology development. Shaping sustainable relations between consumers and technologies

NARCIS (Netherlands)

Slob, A.F.L.; Verbeek, P.P.

2006-01-01

Environmental policy has long been determined by a dichotomy between technology and behavior. Some approaches stress the importance of technology and technological innovation, while others focus on behavioral change. Each approach has its limitations, however, since technology and behavior often

An Introduction to Biometrics Technology: Its Place in Technology Education

Science.gov (United States)

Elliott, Stephen J.; Peters, Jerry L.; Rishel, Teresa J.

2004-01-01

The increased utilization of biometrics technology in the past few years has contributed to a strong growth pattern as the technology is used in a variety of facilities, including schools. Due to media exposure, students' familiarity with technology will continue to increase proportionately, which will result in an increased curiosity about…

In-Space Propulsion Technology Program Solar Electric Propulsion Technologies

Science.gov (United States)

Dankanich, John W.

2006-01-01

NASA's In-space Propulsion (ISP) Technology Project is developing new propulsion technologies that can enable or enhance near and mid-term NASA science missions. The Solar Electric Propulsion (SEP) technology area has been investing in NASA s Evolutionary Xenon Thruster (NEXT), the High Voltage Hall Accelerator (HiVHAC), lightweight reliable feed systems, wear testing, and thruster modeling. These investments are specifically targeted to increase planetary science payload capability, expand the envelope of planetary science destinations, and significantly reduce the travel times, risk, and cost of NASA planetary science missions. Status and expected capabilities of the SEP technologies are reviewed in this presentation. The SEP technology area supports numerous mission studies and architecture analyses to determine which investments will give the greatest benefit to science missions. Both the NEXT and HiVHAC thrusters have modified their nominal throttle tables to better utilize diminished solar array power on outbound missions. A new life extension mechanism has been implemented on HiVHAC to increase the throughput capability on low-power systems to meet the needs of cost-capped missions. Lower complexity, more reliable feed system components common to all electric propulsion (EP) systems are being developed. ISP has also leveraged commercial investments to further validate new ion and hall thruster technologies and to potentially lower EP mission costs.

Genealogies of Modern Technology

DEFF Research Database (Denmark)

Riis, Søren

2008-01-01

Does modern technology differ from ancient technology and does it have a unique essence? This twofold question opens one of Martin Heidegger's most influential philosophical inquiries, The Question Concerning Technology. The answer Heidegger offers has inspired various critiques and appraisals from...... a vast number of contemporary scholars of technology.1 Heidegger's answer is traditionally thought to suggest a great difference between ancient and modern technology. However, by re-examining Heidegger's text, it is possible to discover previously ignored or misunderstood lines of thoughts that affirm...... a multi-stable interpretation of the origin of modern technology. In what follows, we shall see how The Question Concerning Technology in fact supports three different genealogies of modern technology...

Globalization & technology

DEFF Research Database (Denmark)

Narula, Rajneesh

Technology and globalization are interdependent processes. Globalization has a fundamental influence on the creation and diffusion of technology, which, in turn, affects the interdependence of firms and locations. This volume examines the international aspect of this interdependence at two levels...... of innovation" understanding of learning. Narula and Smith reconcile an important paradox. On the one hand, locations and firms are increasingly interdependent through supranational organisations, regional integration, strategic alliances, and the flow of investments, technologies, ideas and people...

Technology alliances

International Nuclear Information System (INIS)

Torgerson, D.F.; Boczar, P.G.; Kugler, G.

1991-10-01

In the field of nuclear technology, Canada and Korea developed a highly successful relationship that could serve as a model for other high-technology industries. This is particularly significant when one considers the complexity and technical depth required to design, build and operate a nuclear reactor. This paper will outline the overall framework for technology transfer and cooperation between Canada and Korea, and will focus on cooperation in nuclear R and D between the two countries

SHARED TECHNOLOGY TRANSFER PROGRAM

Energy Technology Data Exchange (ETDEWEB)

GRIFFIN, JOHN M. HAUT, RICHARD C.

2008-03-07

The program established a collaborative process with domestic industries for the purpose of sharing Navy-developed technology. Private sector businesses were educated so as to increase their awareness of the vast amount of technologies that are available, with an initial focus on technology applications that are related to the Hydrogen, Fuel Cells and Infrastructure Technologies (Hydrogen) Program of the U.S. Department of Energy. Specifically, the project worked to increase industry awareness of the vast technology resources available to them that have been developed with taxpayer funding. NAVSEA-Carderock and the Houston Advanced Research Center teamed with Nicholls State University to catalog NAVSEA-Carderock unclassified technologies, rated the level of readiness of the technologies and established a web based catalog of the technologies. In particular, the catalog contains technology descriptions, including testing summaries and overviews of related presentations.

General survey of technology management

Energy Technology Data Exchange (ETDEWEB)

Shin, Yong Ha; Jang Dong Hun; Lee, In Woo

1999-02-15

The content of this book are technology and management, conception of technology management, feature and model of technology management system, elements of technology management, performance measurement of technology management, connectivity between technology and business function, system management of technology, manpower management, readership and technology management, motivation and technology management, management of technical investment evaluation, new item development and marketing, product liability management, intellectual property rights and transfer of technology.

General survey of technology management

International Nuclear Information System (INIS)

Shin, Yong Ha; Jang Dong Hun; Lee, In Woo

1999-02-01

The content of this book are technology and management, conception of technology management, feature and model of technology management system, elements of technology management, performance measurement of technology management, connectivity between technology and business function, system management of technology, manpower management, readership and technology management, motivation and technology management, management of technical investment evaluation, new item development and marketing, product liability management, intellectual property rights and transfer of technology.

Responsible technology acceptance

DEFF Research Database (Denmark)

Toft, Madeleine Broman; Schuitema, Geertje; Thøgersen, John

2014-01-01

As a response to climate change and the desire to gain independence from imported fossil fuels, there is a pressure to increase the proportion of electricity from renewable sources which is one of the reasons why electricity grids are currently being turned into Smart Grids. In this paper, we focus...... on private consumers’ acceptance of having Smart Grid technology installed in their home. We analyse acceptance in a combined framework of the Technology Acceptance Model and the Norm Activation Model. We propose that individuals are only likely to accept Smart Grid technology if they assess usefulness...... in terms of a positive impact for society and the environment. Therefore, we expect that Smart Grid technology acceptance can be better explained when the well-known technology acceptance parameters included in the Technology Acceptance Model are supplemented by moral norms as suggested by the Norm...

Technology transfer by multinationals

OpenAIRE

Kostyantyn Zuzik

2003-01-01

The paper analyses the issue of technology transfer by multinational corporations. The following questions are explored: (a) world market of technologies, the role of MNCs (b) Choice of the technology transfer mode, Dunning's OLI-theory as a factor of the choice of the mode of transfer (c) measurement and profitability of technology transfer (d) transfer of technology through partnerships, JVs, alliances and through M&As (e) aspects of technology transfer by services multinationals. Paper uti...

Occurrence of Fatal and Nonfatal Adverse Outcomes after Heart Transplantation in Patients with Pretransplant Noncytotoxic HLA Antibodies

Directory of Open Access Journals (Sweden)

Luciano Potena

2013-01-01

Full Text Available HLA antibodies (HLA ab in transplant candidates have been associated with poor outcome. However, clinical relevance of noncytotoxic antibodies after heart transplant (HT is controversial. By using a Luminex-based HLA screening, we retested pretransplant sera from HT recipients testing negative for cytotoxic HLA ab and for prospective crossmatch. Out of the 173 consecutive patients assayed (52±13y; 16% females; 47% ischemic etiology, 32 (18% showed pretransplant HLA ab, and 12 (7% tested positive against both class I and class II HLA. Recipients with any HLA ab had poorer survival than those without (65±9 versus 82±3%; P=0.02, accounting for a doubled independent mortality risk (P=0.04. In addition, HLA-ab detection was associated with increased prevalence of early graft failure (35 versus 15%; P=0.05 and late cellular rejection (29 versus 11%; P=0.03. Of the subgroup of 37 patients suspected for antibody mediated rejection (AMR, the 9 with pretransplant HLA ab were more likely to display pathological AMR grade 2 (P=0.04. By an inexpensive, luminex-based, HLA-screening assay, we were able to detect non-cytotoxic HLA ab predicting fatal and nonfatal adverse outcomes after heart transplant. Allocation strategies and desensitization protocols need to be developed and prospectively tested in these patients.

Robotics Technology Crosscutting Program. Technology summary

International Nuclear Information System (INIS)

1995-06-01

The Robotics Technology Development Program (RTDP) is a needs-driven effort. A length series of presentations and discussions at DOE sites considered critical to DOE's Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the resulting robotics needs assessment revealed several common threads running through the sites: Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination and Dismantlement (D and D). The RTDP Group also realized that some of the technology development in these four areas had common (Cross Cutting-CC) needs, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT and E) process urged an additional organizational breakdown between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). These factors lead to the formation of the fifth application area for Crosscutting and Advanced Technology (CC and AT) development. The RTDP is thus organized around these application areas -- TWR, CAA, MWO, D and D, and CC and AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas

Robotics Technology Crosscutting Program. Technology summary

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-06-01

The Robotics Technology Development Program (RTDP) is a needs-driven effort. A length series of presentations and discussions at DOE sites considered critical to DOE`s Environmental Restoration and Waste Management (EM) Programs resulted in a clear understanding of needed robotics applications toward resolving definitive problems at the sites. A detailed analysis of the resulting robotics needs assessment revealed several common threads running through the sites: Tank Waste Retrieval (TWR), Contaminant Analysis Automation (CAA), Mixed Waste Operations (MWO), and Decontamination and Dismantlement (D and D). The RTDP Group also realized that some of the technology development in these four areas had common (Cross Cutting-CC) needs, for example, computer control and sensor interface protocols. Further, the OTD approach to the Research, Development, Demonstration, Testing, and Evaluation (RDDT and E) process urged an additional organizational breakdown between short-term (1--3 years) and long-term (3--5 years) efforts (Advanced Technology-AT). These factors lead to the formation of the fifth application area for Crosscutting and Advanced Technology (CC and AT) development. The RTDP is thus organized around these application areas -- TWR, CAA, MWO, D and D, and CC and AT -- with the first four developing short-term applied robotics. An RTDP Five-Year Plan was developed for organizing the Program to meet the needs in these application areas.

Transferable site remediation technologies developed by U.S. DOE Office of Science and Technology

International Nuclear Information System (INIS)

Anderson, T.D.

1996-01-01

To provide needed technologies for site remediation, the US Department of Energy's Office of Environmental Management, Office of Science and Technology (OST) is developing technologies to address environmental problems associated with hazardous and radioactive contaminants in soil and groundwater. The Technology Investment Decision model serves as a framework for technology management in OST. Seven technology maturation stages are used in the model. These stages run from basic research through implementation. The Innovative Technology Summary Reports (ITSRs) provide a technical synopsis of an individual technology that has been developed. An ITSR is prepared for each technology that is successfully demonstrated in the field. The information required to produce an ITSR is collected as the technology matures through the Technology Investment Decision Process. As of July 1996 there have been thirteen ITSRs completed. This paper describes those thirteen technologies

Technology readiness levels and technology status for selected long term/high payoff technologies on the RLV program

Science.gov (United States)

Rosmait, Russell L.

1996-01-01

The development of a new space transportation system in a climate of constant budget cuts and staff reductions can be and is a difficult task. It is no secret that NASA's current launching system consumes a very large portion of NASA funding and requires a large army of people to operate & maintain the system. The new Reusable Launch Vehicle (RLV) project and it's programs are faced with a monumental task of making the cost of access to space dramatically lower and more efficient than NASA's current system. With pressures from congressional budget cutters and also increased competition and loss of market share from international agencies RLV's first priority is to develop a 'low-cost, reliable transportation to earth orbit.' One of the RLV's major focus in achieving low-cost, reliable transportation to earth orbit is to rely on the maturing of advanced technologies. The technologies for the RLV are numerous and varied. Trying to assess their current status, within the RLV development program is paramount. There are several ways to assess these technologies. One way is through the use of Technology Readiness Levels (TRL's). This project focused on establishing current (summer 95) 'worst case' TRL's for six selected technologies that are under consideration for use within the RLV program. The six technologies evaluated were Concurrent Engineering, Embedded Sensor Technology, Rapid Prototyping, Friction Stir Welding, Thermal Spray Coatings, and VPPA Welding.

NASA technology applications team: Applications of aerospace technology

Science.gov (United States)

1993-01-01

This report covers the activities of the Research Triangle Institute (RTI) Technology Applications Team for the period 1 October 1992 through 30 September 1993. The work reported herein was supported by the National Aeronautics and Space Administration (NASA), Contract No. NASW-4367. Highlights of the RTI Applications Team activities over the past year are presented in Section 1.0. The Team's progress in fulfilling the requirements of the contract is summarized in Section 2.0. In addition to our market-driven approach to applications project development, RTI has placed increased effort on activities to commercialize technologies developed at NASA Centers. These Technology Commercialization efforts are summarized in Section 3.0. New problem statements prepared by the Team in the reporting period are presented in Section 4.0. The Team's transfer activities for ongoing projects with the NASA Centers are presented in Section 5.0. Section 6.0 summarizes the status of four add-on tasks. Travel for the reporting period is described in Section 7.0. The RTI Team staff and consultants and their project responsibilities are listed in Appendix A. Appendix B includes Technology Opportunity Announcements and Spinoff! Sheets prepared by the Team while Appendix C contains a series of technology transfer articles prepared by the Team.

IT-BT convergence technology

International Nuclear Information System (INIS)

2012-12-01

This book explains IT-BT convergence technology as the future technology, which includes a prolog, easy IT-BT convergence technology that has infinite potentials for new value, policy of IT-BT convergence technology showing the potential of smart Korea, IT-BT convergence opening happy future, for the new future of IT powerful nation Korea with IT-BT convergence technology and an epilogue. This book reveals the conception, policy, performance and future of IT-BT convergence technology.

Heterogeneous technologies, strategic groups and environmental efficiency technology gaps for European countries

International Nuclear Information System (INIS)

Kounetas, Konstantinos

2015-01-01

This paper measures technology (TG) and environmental efficiency technology gaps (EETGs) in 25 European countries over two distinct periods 2002 and 2008 examining the possible effect of adopted environmental regulations and the Kyoto protocol commitments on environmental efficiency technology gaps. However, the introduction of the metafrontier in our analysis puts into our discussion the role of heterogeneous technologies and its effect on the above-mentioned measures. Employing a directional distance function, we investigate whether there is an actual difference, in terms of environmental efficiency and efficiency performance, among European countries considering the technological frontiers under which they operate. The construction of individual frontiers has been realized employing a large number of variables that are highly correlated with countries' learning and absorbing capacity, new technological knowledge and using economic theory and classical frontier discrimination like developed vs. developing, North vs. South and participation in the Eurozone or not. The overall results indicate a crucial role of heterogeneous technologies for technology gaps in both periods. Moreover, a significant decrease for both measures, although in different percent, has been recorded emphasizing the key role of knowledge spillovers. -- Highlights: •We estimate technology gaps (TGs) for 25 EU countries in two distinct periods. •We estimate environmental efficiency technology gaps (EETGs). •We consider countries' technological capabilities with R&D, innovation and eco-innovation. •We test the effect of different frontier constitutions on TGs-EETGs. •We denote the specific role of knowledge spillovers

The Complete Picture: "Standards for Technological Literacy" and "Advancing Excellence in Technological Literacy."

Science.gov (United States)

Technology Teacher, 2003

2003-01-01

Provides an overview of the "Standards for Technological Literacy: Content for the Study of Technology" (STL) and "Advancing Excellence in Technological Literacy: Student Assessment, Professional Development, and Program Standards" (AETL). Shows how the documents work together to advance the technological literacy of technology educators and K-12…

Plasma technology

International Nuclear Information System (INIS)

Drouet, M.G.

1984-03-01

IREQ was contracted by the Canadian Electrical Association to review plasma technology and assess the potential for application of this technology in Canada. A team of experts in the various aspects of this technology was assembled and each team member was asked to contribute to this report on the applications of plasma pertinent to his or her particular field of expertise. The following areas were examined in detail: iron, steel and strategic-metals production; surface treatment by spraying; welding and cutting; chemical processing; drying; and low-temperature treatment. A large market for the penetration of electricity has been identified. To build up confidence in the technology, support should be provided for selected R and D projects, plasma torch demonstrations at full power, and large-scale plasma process testing

Technology transfer and international development: Materials and manufacturing technology

Science.gov (United States)

1982-01-01

Policy oriented studies on technological development in several relatively advanced developing countries were conducted. Priority sectors defined in terms of technological sophistication, capital intensity, value added, and export potential were studied in Brazil, Venezuela, Israel, and Korea. The development of technological policy alternatives for the sponsoring country is assessed. Much emphasis is placed on understanding the dynamics of the sectors through structured interviews with a large sample of firms in the leading manufacturing and materials processing sectors.

Large Deployable Reflector (LDR) system concept and technology definition study. Volume 2: Technology assessment and technology development plan

Science.gov (United States)

Agnew, Donald L.; Jones, Peter A.

1989-01-01

A study was conducted to define reasonable and representative LDR system concepts for the purpose of defining a technology development program aimed at providing the requisite technological capability necessary to start LDR development by the end of 1991. This volume presents thirteen technology assessments and technology development plans, as well as an overview and summary of the LDR concepts. Twenty-two proposed augmentation projects are described (selected from more than 30 candidates). The five LDR technology areas most in need of supplementary support are: cryogenic cooling; astronaut assembly of the optically precise LDR in space; active segmented primary mirror; dynamic structural control; and primary mirror contamination control. Three broad, time-phased, five-year programs were synthesized from the 22 projects, scheduled, and funding requirements estimated.

Comparative Assessment of Anti-HLA Antibodies Using Two Commercially Available Luminex-Based Assays

Directory of Open Access Journals (Sweden)

Kevin J. Clerkin, MD, MSc

2017-11-01

Conclusions. Immucor and One Lambda/ThermoFisher assays have a similar, albeit nonidentical, ability to detect anti-HLA Ab. Although the correlation between the assays was present, significant variances exist, some of which can be explained by a dilution-sensitive “prozone” effect.

Comparative Assessment of Anti-HLA Antibodies Using Two Commercially Available Luminex-Based Assays.

Science.gov (United States)

Clerkin, Kevin J; See, Sarah B; Farr, Maryjane A; Restaino, Susan W; Serban, Geo; Latif, Farhana; Li, Lingzhi; Colombo, Paolo C; Vlad, George; Ray, Bryan; Vasilescu, Elena R; Zorn, Emmanuel

2017-11-01

Allospecific anti-HLA antibodies (Abs) are associated with rejection of solid organ grafts. The 2 main kits to detect anti-HLA Ab in patient serum are commercialized by Immucor and One Lambda/ThermoFisher. We sought to compare the performance of both platforms. Background-adjusted mean fluorescence intensity (MFI) values were used from both platforms to compare sera collected from 125 pretransplant and posttransplant heart and lung transplant recipients. Most HLA class I (94.5%) and HLA class II (89%) Abs with moderate to high MFI titer (≥4000) were detected by both assays. A modest correlation was observed between MFI values obtained from the 2 assays for both class I ( r = 0.3, r 2 = 0.09, P < 0.0001) and class II Ab ( r = 0.707, r 2 = 0.5, P < 0.0001). Both assays detected anti-class I and II Ab that the other did not; however, no specific HLA allele was detected preferentially by either of the 2 assays. For a limited number of discrepant sera, dilution resulted in comparable reactivity profiles between the 2 platforms. Immucor and One Lambda/ThermoFisher assays have a similar, albeit nonidentical, ability to detect anti-HLA Ab. Although the correlation between the assays was present, significant variances exist, some of which can be explained by a dilution-sensitive "prozone" effect.

Performance of commercial platforms for rapid genotyping of polymorphisms affecting warfarin dose.

Science.gov (United States)

King, Cristi R; Porche-Sorbet, Rhonda M; Gage, Brian F; Ridker, Paul M; Renaud, Yannick; Phillips, Michael S; Eby, Charles

2008-06-01

Initiation of warfarin therapy is associated with bleeding owing to its narrow therapeutic window and unpredictable therapeutic dose. Pharmacogenetic-based dosing algorithms can improve accuracy of initial warfarin dosing but require rapid genotyping for cytochrome P-450 2C9 (CYP2C9) *2 and *3 single nucleotide polymorphisms (SNPs) and a vitamin K epoxide reductase (VKORC1) SNP. We evaluated 4 commercial systems: INFINITI analyzer (AutoGenomics, Carlsbad, CA), Invader assay (Third Wave Technologies, Madison, WI), Tag-It Mutation Detection assay (Luminex Molecular Diagnostics, formerly Tm Bioscience, Toronto, Canada), and Pyrosequencing (Biotage, Uppsala, Sweden). We genotyped 112 DNA samples and resolved any discrepancies with bidirectional sequencing. The INFINITI analyzer was 100% accurate for all SNPs and required 8 hours. Invader and Tag-It were 100% accurate for CYP2C9 SNPs, 99% accurate for VKORC1 -1639/3673 SNP, and required 3 hours and 8 hours, respectively. Pyrosequencing was 99% accurate for CYP2C9 *2, 100% accurate for CYP2C9 *3, and 100% accurate for VKORC1 and required 4 hours. Current commercial platforms provide accurate and rapid genotypes for pharmacogenetic dosing during initiation of warfarin therapy.

Multiplexed Molecular Assays for Rapid Rule-Out of Foot-and-Mouth Disease

Energy Technology Data Exchange (ETDEWEB)

Lenhoff, R; Naraghi-Arani, P; Thissen, J; Olivas, J; Carillo, C; Chinn, C; Rasmussen, M; Messenger, S; Suer, L; Smith, S M; Tammero, L; Vitalis, E; Slezak, T R; Hullinger, P J; Hindson, B J; Hietala, S; Crossley, B; Mcbride, M

2007-06-26

A nucleic acid-based multiplexed assay was developed that combines detection of foot-and-mouth disease virus (FMDV) with rule-out assays for two other foreign animal diseases and four domestic animal diseases that cause vesicular or ulcerative lesions indistinguishable from FMDV infection in cattle, sheep and swine. The FMDV 'look-alike' diagnostic assay panel contains five PCR and twelve reverse transcriptase PCR (RT-PCR) signatures for a total of seventeen simultaneous PCR amplifications for seven diseases plus incorporating four internal assay controls. It was developed and optimized to amplify both DNA and RNA viruses simultaneously in a single tube and employs Luminex{trademark} liquid array technology. Assay development including selection of appropriate controls, a comparison of signature performance in single and multiplex testing against target nucleic acids, as well of limits of detection for each of the individual signatures is presented. While this assay is a prototype and by no means a comprehensive test for FMDV 'look-alike' viruses, an assay of this type is envisioned to have benefit to a laboratory network in routine surveillance and possibly for post-outbreak proof of freedom from foot-and-mouth disease.

Current and New Approaches in GMO Detection: Challenges and Solutions

Directory of Open Access Journals (Sweden)

Marie-Alice Fraiture

2015-01-01

Full Text Available In many countries, genetically modified organisms (GMO legislations have been established in order to guarantee the traceability of food/feed products on the market and to protect the consumer freedom of choice. Therefore, several GMO detection strategies, mainly based on DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR is the method of choice for the enforcement laboratories in GMO routine analysis. However, given the increasing number and diversity of GMO developed and put on the market around the world, some technical hurdles could be encountered with the qPCR technology, mainly owing to its inherent properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification, simultaneous detections of multiple GM targets (e.g., PCR capillary gel electrophoresis, microarray, and Luminex, more accurate quantification of GM targets (e.g., digital PCR, or characterization of partially known (e.g., DNA walking and Next Generation Sequencing (NGS or unknown (e.g., NGS GMO. The benefits and drawbacks of these methods are discussed in this review.

Current and new approaches in GMO detection: challenges and solutions.

Science.gov (United States)

Fraiture, Marie-Alice; Herman, Philippe; Taverniers, Isabel; De Loose, Marc; Deforce, Dieter; Roosens, Nancy H

2015-01-01

In many countries, genetically modified organisms (GMO) legislations have been established in order to guarantee the traceability of food/feed products on the market and to protect the consumer freedom of choice. Therefore, several GMO detection strategies, mainly based on DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. However, given the increasing number and diversity of GMO developed and put on the market around the world, some technical hurdles could be encountered with the qPCR technology, mainly owing to its inherent properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification), simultaneous detections of multiple GM targets (e.g., PCR capillary gel electrophoresis, microarray, and Luminex), more accurate quantification of GM targets (e.g., digital PCR), or characterization of partially known (e.g., DNA walking and Next Generation Sequencing (NGS)) or unknown (e.g., NGS) GMO. The benefits and drawbacks of these methods are discussed in this review.

A multiplex branched DNA assay for parallel quantitative gene expression profiling.

Science.gov (United States)

Flagella, Michael; Bui, Son; Zheng, Zhi; Nguyen, Cung Tuong; Zhang, Aiguo; Pastor, Larry; Ma, Yunqing; Yang, Wen; Crawford, Kimberly L; McMaster, Gary K; Witney, Frank; Luo, Yuling

2006-05-01

We describe a novel method to quantitatively measure messenger RNA (mRNA) expression of multiple genes directly from crude cell lysates and tissue homogenates without the need for RNA purification or target amplification. The multiplex branched DNA (bDNA) assay adapts the bDNA technology to the Luminex fluorescent bead-based platform through the use of cooperative hybridization, which ensures an exceptionally high degree of assay specificity. Using in vitro transcribed RNA as reference standards, we demonstrated that the assay is highly specific, with cross-reactivity less than 0.2%. We also determined that the assay detection sensitivity is 25,000 RNA transcripts with intra- and interplate coefficients of variance of less than 10% and less than 15%, respectively. Using three 10-gene panels designed to measure proinflammatory and apoptosis responses, we demonstrated sensitive and specific multiplex gene expression profiling directly from cell lysates. The gene expression change data demonstrate a high correlation coefficient (R(2)=0.94) compared with measurements obtained using the single-plex bDNA assay. Thus, the multiplex bDNA assay provides a powerful means to quantify the gene expression profile of a defined set of target genes in large sample populations.

Soil washing technology evaluation

International Nuclear Information System (INIS)

Suer, A.

1995-04-01

Environmental Restoration Engineering (ERE) continues to review innovative, efficient, and cost effective technologies for SRS soil and/or groundwater remediation. As part of this effort, this technical evaluation provides review and the latest information on the technology for SRS soil remediation. Additional technology evaluation reports will be issued periodically to update these reports. The purpose of this report is to review the soil washing technology and its potential application to SRS soil remediation. To assess whether the Soil Washing technology is a viable option for SRS soil remediation, it is necessary to review the technology/process, technology advantages/limitations, performance, applications, and cost analysis

Advancement in Engineering Technology

DEFF Research Database (Denmark)

Kalia, Kartik; Rehman, M. Atiqur; Hussain, Dil muhammed Akbar

2016-01-01

In this paper we will be discussing about the impact of technology on our daily lives. How everybody is dependent upon technology in one or other way. Methods/Statistical Analysis: Technology has played a significant role in the evolution of the society. Science has produced many new ideas...... but to harvest those ideas, technology is a must. With the huge requirement of engineering equipment's, the industry needs specialists who can manage and operate these technologies. Detailed information about the merits and demerits of technology is also mentioned in this paper. Findings: Technology has affected...... the environment on a great scale; in some cases, technology is even replacing human being or use of manpower. So proper counter measures have been mentioned, which can be used to control and limit harmful effect....

Technology licensing in China

DEFF Research Database (Denmark)

Wang, Yuandi; Li-Ying, Jason; Chen, Jin

2015-01-01

We explore the landscape of technology licensing among Chinese entities in the period 2000–12, using a unique database on technological licensing from the State Intellectual Property Office of China. We find that: first, among Chinese licensee organizations, firms have dominated in terms...... of the number of licensed technologies; second, the geographical distribution of licensed technologies among the provinces has gradually reached a new quantitative balance; third, utility models are the most popular technologies to be licensed and the majority of technology licensing in China has been between...... Chinese entities, and most transactions have been local within provinces; and finally, Chinese firms have gradually in-licensed newer and newer technologies, but the technologies in-licensed from foreign sources are by no means state-of-the-art. We make several suggestions for innovation policy...

Technology Applications Team: Applications of aerospace technology

Science.gov (United States)

1993-01-01

Highlights of the Research Triangle Institute (RTI) Applications Team activities over the past quarter are presented in Section 1.0. The Team's progress in fulfilling the requirements of the contract is summarized in Section 2.0. In addition to our market-driven approach to applications project development, RTI has placed increased effort on activities to commercialize technologies developed at NASA Centers. These Technology Commercialization efforts are summarized in Section 3.0. New problem statements prepared by the Team in the reporting period are presented in Section 4.0. The Team's transfer activities for ongoing projects with the NASA Centers are presented in Section 5.0. Section 6.0 summarizes the status of four add-on tasks. Travel for the reporting period is described in Section 7.0. The RTI Team staff and consultants and their project responsibilities are listed in Appendix A. The authors gratefully acknowledge the contributions of many individuals to the RTI Technology Applications Team program. The time and effort contributed by managers, engineers, and scientists throughout NASA were essential to program success. Most important to the program has been a productive working relationship with the NASA Field Center Technology Utilization (TU) Offices. The RTI Team continues to strive for improved effectiveness as a resource to these offices. Industry managers, technical staff, medical researchers, and clinicians have been cooperative and open in their participation. The RTI Team looks forward to continuing expansion of its interaction with U.S. industry to facilitate the transfer of aerospace technology to the private sector.

Sustainable technology transfer

NARCIS (Netherlands)

Punter, H.T.; Krikhaar, R.L.; Bril, R.J.

2006-01-01

In this position paper we address the issue of transferring a technology from research into an industrial organization by presenting a refined process for technology transfer. Based on over two decades of industrial experience, we identified the need for a dedicated technology engineering phase for

Casting Technology.

Science.gov (United States)

Wright, Michael D.; And Others

1992-01-01

Three articles discuss (1) casting technology as it relates to industry, with comparisons of shell casting, shell molding, and die casting; (2) evaporative pattern casting for metals; and (3) high technological casting with silicone rubber. (JOW)

Technology meets research 60 years of CERN technology : selected highlights

CERN Document Server

Taylor, Thomas; Treille, Daniel; Wenninger, Horst

2017-01-01

"Big" science and advanced technology are known to cross-fertilize. This book emphasizes the interplay between particle physics and technology at CERN that has led to breakthroughs in both research and technology over the laboratory's first 60 years. The innovations, often the work of individuals or by small teams, are illustrated with highlights describing selected technologies from the domains of accelerators and detectors. The book also presents the framework and conditions prevailing at CERN that enabled spectacular advances in technology and contributed to propel the European organization into the league of leading research laboratories in the world. While the book is specifically aimed at providing information for the technically interested general public, more expert readers may also appreciate the broad variety of subjects presented. Ample references are given for those who wish to further explore a given topic.

Robotics Technology Development Program Cross Cutting and Advanced Technology

International Nuclear Information System (INIS)

Harrigan, R.W.; Horschel, D.S.

1994-01-01

Need-based cross cutting technology is being developed which is broadly applicable to the clean up of hazardous and radioactive waste within the US Department of Energy's complex. Highly modular, reusable technologies which plug into integrated system architectures to meet specific robotic needs result from this research. In addition, advanced technologies which significantly extend current capabilities such as automated planning and sensor-based control in unstructured environments for remote system operation are also being developed and rapidly integrated into operating systems

Theme: Emerging Technologies.

Science.gov (United States)

Malpiedi, Barbara J.; And Others

1989-01-01

Consists of six articles discussing the effect of emerging technologies on agriculture. Specific topics include (1) agriscience programs, (2) the National Conference on Agriscience and Emerging Occupations and Technologies, (3) biotechnology, (4) program improvement through technology, (5) the Agriscience Teacher of the Year program, and (6)…

Moralizing Food Technology

DEFF Research Database (Denmark)

Coff, Christian Eyde

2015-01-01

Food technologies are common on many levels in society and used by both food professionals and consumers. Food technologies are not neutral. They inform and shape the behaviour of people. This paper presents a theoretical framework for analysing the mediating role of food technology and its influ...

The role of surgeons in identifying emerging technologies for health technology assessment.

Science.gov (United States)

Stafinski, Tania; Topfer, Leigh-Ann; Zakariasen, Ken; Menon, Devidas

2010-04-01

Health technology assessment (HTA) is a tool intended to help policy-makers decide which technologies to fund. However, given the proliferation of new technologies, it is not possible to undertake an HTA of each one before it becomes funded. Consequently, "horizon-scanning" processes have been developed to identify emerging technologies that are likely to have a substantial impact on clinical practice. Although the importance of physicians in the adoption of new technologies is well recognized, their role in horizon scanning in Canada has been limited. The purpose of this project was to pilot an approach to engage physicians, specifically surgeons, in provincial horizon-scanning activities. We invited 18 surgeons from Alberta's 2 medical schools to a horizon-scanning workshop to solicit their views on emerging technologies expected to impact surgical practice within the next 5 years and/or the importance of different attributes or characteristics of new technologies. Surgeons, regardless of specialty, identified developments designed to enhance existing minimally invasive surgical techniques, such as endoscopic, robotic and image-guided surgery. Several nonsurgical areas, including molecular genetics and nano technology, were also identified. Of the 13 technology attributes discussed, safety or risk, effectiveness and feasibility were rated as most important. Lastly, participating surgeons expressed an interest in becoming further involved in local HTA initiatives. Surgeons, as adopters and users of health technologies, represent an important and accessible information source for identifying emerging technologies for HTA. A more formal, ongoing relationship between the government, HTA and surgeons may help to optimize the use of HTA resources.

Technology Transition for Hybrid Warfare

Science.gov (United States)

2010-02-16

and Iraq. At the same time, the science and technology base must provide the disruptive technologies to defeat future conventional enemies. This... disruptive technologies will be needed to retain long-term technological superiority in conventional warfare. Incremental improvement is the most...technology to be missed. Disruptive technologies are the second type of technological change and involve revolutionary concepts involving large technological

Emerging Energy-Efficient Technologies in Buildings Technology Characterizations for Energy Modeling

Energy Technology Data Exchange (ETDEWEB)

Hadley, SW

2004-10-11

The energy use in America's commercial and residential building sectors is large and growing. Over 38 quadrillion Btus (Quads) of primary energy were consumed in 2002, representing 39% of total U.S. energy consumption. While the energy use in buildings is expected to grow to 52 Quads by 2025, a large number of energy-related technologies exist that could curtail this increase. In recent years, improvements in such items as high efficiency refrigerators, compact fluorescent lights, high-SEER air conditioners, and improved building shells have all contributed to reducing energy use. Hundreds of other technology improvements have and will continue to improve the energy use in buildings. While many technologies are well understood and are gradually penetrating the market, more advanced technologies will be introduced in the future. The pace and extent of these advances can be improved through state and federal R&D. This report focuses on the long-term potential for energy-efficiency improvement in buildings. Five promising technologies have been selected for description to give an idea of the wide range of possibilities. They address the major areas of energy use in buildings: space conditioning (33% of building use), water heating (9%), and lighting (16%). Besides describing energy-using technologies (solid-state lighting and geothermal heat pumps), the report also discusses energy-saving building shell improvements (smart roofs) and the integration of multiple energy service technologies (CHP packaged systems and triple function heat pumps) to create synergistic savings. Finally, information technologies that can improve the efficiency of building operations are discussed. The report demonstrates that the United States is not running out of technologies to improve energy efficiency and economic and environmental performance, and will not run out in the future. The five technology areas alone can potentially result in total primary energy savings of between 2 and

Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation.

Science.gov (United States)

Stephen, Laurie; Guest, Paul C

2018-01-01

Multiplex immunoassays are used for rapid profiling of biomarker proteins and small molecules in biological fluids. The advantages over single immunoassays include lower sample consumption, cost, and labor. This chapter details a protocol to develop a 5-plex assay for glucagon-like peptide 1, growth hormone, insulin, leptin, and thyroid-stimulating hormone on the Luminex ® platform. The results of the analysis of insulin in normal control subjects are given due to the important role of this hormone in nutritional programming diseases.

Technology transfer

International Nuclear Information System (INIS)

1998-01-01

On the base of technological opportunities and of the environmental target of the various sectors of energy system this paper intend to conjugate the opportunity/objective with economic and social development through technology transfer and information dissemination [it

Technology transfer for adaptation

Science.gov (United States)

Biagini, Bonizella; Kuhl, Laura; Gallagher, Kelly Sims; Ortiz, Claudia

2014-09-01

Technology alone will not be able to solve adaptation challenges, but it is likely to play an important role. As a result of the role of technology in adaptation and the importance of international collaboration for climate change, technology transfer for adaptation is a critical but understudied issue. Through an analysis of Global Environment Facility-managed adaptation projects, we find there is significantly more technology transfer occurring in adaptation projects than might be expected given the pessimistic rhetoric surrounding technology transfer for adaptation. Most projects focused on demonstration and early deployment/niche formation for existing technologies rather than earlier stages of innovation, which is understandable considering the pilot nature of the projects. Key challenges for the transfer process, including technology selection and appropriateness under climate change, markets and access to technology, and diffusion strategies are discussed in more detail.

Editorial: Advanced learning technologies

Directory of Open Access Journals (Sweden)

Yu-Ju Lan

2012-03-01

Full Text Available Recent rapid development of advanced information technology brings high expectations of its potential to improvement and innovations in learning. This special issue is devoted to using some of the emerging technologies issues related to the topic of education and knowledge sharing, involving several cutting edge research outcomes from recent advancement of learning technologies. Advanced learning technologies are the composition of various related technologies and concepts such as mobile technologies and social media towards learner centered learning. This editorial note provides an overview of relevant issues discussed in this special issue.

Environmental Technologies Summary Book

International Nuclear Information System (INIS)

2009-02-01

This book lists the companies and their technology, which have new excellent technology authentication and technology verification. They are as in the following : sewage advanced treatment technology using a three-stage Bio-Ceramic Filtration by Shinwoo engineering.co.kr, Twist Filter by Sungshin engineering.co.kr, Sewage advanced treatment technology using CIMEN-DOC by Taeyeong/CI biotech.co.kr, DeNipho using pump ejector and Bio Green Media by Green Technology.co.kr, Automatic integrated management system using Envi-SIS by Sallasanup.com Kozone.co.kr and Geoworks.co.kr.

A Study on the Linkage between Nano Fusion Technology and Nuclear Technology

International Nuclear Information System (INIS)

Jeong, Ik; Lim, Chae Young; Lee, Jong Hee

2009-02-01

1) A survey of national energy policy trends in major nation - to secure renewal energy in the level of making a plan to supply national energy in the future - Tendency of energy policy based on Europe 2) A survey of the nano technology development - Status of major nano technology development - Developmental direction of nano technology related to nuclear energy 3) the nano technology development related with nuclear - high-temperature nuclear reactor by applying nano science and technology under quick development - materials required to high-level radioactive wastes treatment facility - develop materials of nuclear fusion facility in the long-term view 4) Innovation system of nano technology - Energy source -> conversion to energy -> distribution of energy -> energy storage -> energy use

Teaching with Technology

Science.gov (United States)

Attard, Catherine

2011-01-01

New technologies continue to change every aspect of home, life and work: the way people communicate, calculate, analyse, shop, make presentations and socialise. "The Australian Curriculum" acknowledges the importance of teaching and learning with technology by including the use of information and communication technology (ICT) as one of…

DECISION ANALYSIS AND TECHNOLOGY ASSESSMENTS FOR METAL AND MASONRY DECONTAMINATION TECHNOLOGIES

International Nuclear Information System (INIS)

Ebadian, M.A.

1999-01-01

The purpose of this investigation was to conduct a comparative analysis of innovative technologies for the non-aggressive removal of coatings from metal and masonry surfaces and the aggressive removal of one-quarter to one-inch thickness of surface from structural masonry. The technologies tested should be capable of being used in nuclear facilities. Innovative decontamination technologies are being evaluated under standard, non-nuclear conditions at the FIU-HCET technology assessment site in Miami, Florida. This study is being performed to support the OST, the Deactivation and Decommissioning (D and D) Focus Area, and the environmental restoration of DOE facilities throughout the DOE complex by providing objective evaluations of currently available decontamination technologies

Moralizing Food Technology

DEFF Research Database (Denmark)

Coff, Christian Eyde

2015-01-01

Food technologies are common on many levels in society and used by both food professionals and consumers. Food technologies are not neutral. They inform and shape the behaviour of people. This paper presents a theoretical framework for analysing the mediating role of food technology and its...... influence on food ethics. Post-phenomenology and the idea of a technologically mediated morality are central theoretical approaches. Four elements are included in the analytical framework: perception, interpretation, intentionality, and mediated morality. The framework is applied to two cases; food safety...

Technology Roadmaps: Cement

Energy Technology Data Exchange (ETDEWEB)

NONE

2009-07-01

To support its roadmap work focusing on key technologies for emissions reductions, the International Energy Agency (IEA) also investigated one particular industry: cement. Cement production includes technologies that are both specific to this industry and those that are shared with other industries (e.g., grinding, fuel preparation, combustion, crushing, transport). An industry specific roadmap provides an effective mechanism to bring together several technology options. It outlines the potential for technological advancement for emissions reductions in one industry, as well as potential cross-industry collaboration.

Development of Technological Profiles for Transfer of Energy- and Resource Saving Technologies

Directory of Open Access Journals (Sweden)

Lysenko, V.S.

2015-01-01

Full Text Available The article deals with the methodological foundations for the development of technological profiles for «System of Transfer of Energy- and Resource Saving Technologies». It is determined that a compliance with the methodology and standards of the European network «Relay Centers» (Innovation Relay Centers — IRC network, since 2008 — EEN, the Russian Technology Transfer Network RTTN and Uk rainian Technology Transfer Network UTTN is the main pri nciple of the development process of technological requests and offers.

Designing Human Technologies

DEFF Research Database (Denmark)

Simonsen, Jesper

and the design process, in ethical and society-related concerns, and in evaluating how designs fulfill needs and solve problems. Designing Human Technologies subscribes to a broad technology concept including information and communication, mobile, environmental/sustainable and energy technologies......Design is increasingly becoming a part of the university curriculum and research agenda. The keynote present and discuss Designing Human Technologies – an initiative aiming at establishing a design oriented main subject area alongside traditional main subject areas such as Natural Science......, the Humanities, and Social Science. The initiative broadens the perspective of IS and recognize reflections on aesthetics, ethics, values, connections to politics, and strategies for enabling a better future as legitimate parts of the research agenda. Designing Human Technologies is a design-oriented Strategic...

Technology Partnership Agreements | NREL

Science.gov (United States)

Partnership Agreements Technology Partnership Agreements Looking for Funding? We do not fund any projects under a technology partnership agreement. The partner provides the necessary resources and, in using technology partnership agreements. See a summary of our Fiscal Year 2017 technology partnership

Maritime Technology

DEFF Research Database (Denmark)

Sørensen, Herman

1997-01-01

Elementary introduction to the subject "Maritime Technology".The contents include drawings, sketches and references in English without any supplementary text.......Elementary introduction to the subject "Maritime Technology".The contents include drawings, sketches and references in English without any supplementary text....

Technology Innovations from NASA's Next Generation Launch Technology Program

Science.gov (United States)

Cook, Stephen A.; Morris, Charles E. K., Jr.; Tyson, Richard W.

2004-01-01

NASA's Next Generation Launch Technology Program has been on the cutting edge of technology, improving the safety, affordability, and reliability of future space-launch-transportation systems. The array of projects focused on propulsion, airframe, and other vehicle systems. Achievements range from building miniature fuel/oxygen sensors to hot-firings of major rocket-engine systems as well as extreme thermo-mechanical testing of large-scale structures. Results to date have significantly advanced technology readiness for future space-launch systems using either airbreathing or rocket propulsion.

Education Technology Transformation

Science.gov (United States)

Kennedy, Mike

2012-01-01

Years ago, as personal computers and other technological advancements began to find their way into classrooms and other educational settings, teachers and administrators sought ways to use new technology to benefit students. The potential for improving education was clear, but the limitations of the available education technology made it difficult…

Clean Cast Steel Technology - Machinability and Technology Transfer

Energy Technology Data Exchange (ETDEWEB)

C. E. Bates; J. A. Griffin

2000-05-01

There were two main tasks in the Clean Cast Steel Technology - Machinability and Technology Transfer Project. These were (1) determine the processing facts that control the machinability of cast steel and (2) determine the ability of ladle stirring to homogenize ladle temperature, reduce the tap and pouring temperatures, and reduce casting scrap.

78 FR 24241 - Nanoscale Science, Engineering, and Technology Subcommittee; Committee on Technology, National...

Science.gov (United States)

2013-04-24

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering, and Technology.... SUMMARY: The National Nanotechnology Coordination Office (NNCO), on behalf of the Nanoscale Science, Engineering, and Technology (NSET) Subcommittee of the Committee on Technology, National Science and...

77 FR 61448 - Nanoscale Science, Engineering and Technology Subcommittee Committee on Technology, National...

Science.gov (United States)

2012-10-09

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering and Technology Subcommittee...: The National Nanotechnology Coordination Office (NNCO), on behalf of the Nanoscale Science, Engineering, and Technology (NSET) Subcommittee of the Committee on Technology, National Science and...

Technology images and concepts of technology in transition. An analysis in the philosophy of technology and general technology; Technikbilder und Technikkonzepte im Wandel. Eine technikphilosophische und allgemeintechnische Analyse

Energy Technology Data Exchange (ETDEWEB)

Banse, G. [Forschungszentrum Karlsruhe GmbH Technik und Umwelt (DE). Inst. fuer Technikfolgen-Abschaetzung und Systemanalyse (ITAS); Meier, B. [Potsdam Univ. (Germany). Inst. fuer Arbeitslehre/Technik; Wolffgramm, H. (eds.)

2002-02-01

This volume contains contributions resulting from an expert discussion on 'Technology Images and Concepts of Technology in Transition - an analysis in the philosophy of technology and general technology. This expert discussion took place on 6 October 2000 at the State Pedagogical Institute Brandenburg in Ludwigsfelde-Struveshof and was conceived and organized co-operatively by the Forschungszentrum Karlsruhe, Institute for Technology Assessment and Systems Analysis, the University of Potsdam, Institute for the Theory of Work/Technology and Professor Horst Wolffgramm, Frankfurt (Oder). It was the aim of the expert discussion to compile, compare and relate the various positions in the philosophy of technology, general technology science, the history of technology and the didactics of technology of the 'conceptualisation' of technology as a basis for generally understanding technology or for a scientifically based 'image of technology' to each other, and then to make them accessible for curricula within a framework of conceiving general technical education at all school levels. The contributions are grouped according to the two main foci of the event: On the one hand they are concerned with determining a contemporary concept of technology ('Image of technology'). One of the aims is to characterize technological change from the historical-genetic perspective and in this way to access technology as a work of mankind, as an important element of our culture. At the same time it is necessary to forecast future developments or to make future paths of development visible to enable the indication of change by basic innovations. Second, on this basis and supported by educational theory, conclusions are drawn for future-oriented technical general education for all students. The main focus in this is on the linkage between goals, content and subject-specific methods. In order to enable the determination of competence of any individual

Promoting renewable energy technologies

International Nuclear Information System (INIS)

Grenaa Jensen, S.

2004-06-01

Technologies using renewable energy sources are receiving increasing interest from both public authorities and power producing companies, mainly because of the environmental advantages they procure in comparison with conventional energy sources. These technologies can be substitution for conventional energy sources and limit damage to the environment. Furthermore, several of the renewable energy technologies satisfy an increasing political goal of self-sufficiency within energy production. The subject of this thesis is promotion of renewable technologies. The primary goal is to increase understanding on how technological development takes place, and establish a theoretical framework that can assist in the construction of policy strategies including instruments for promotion of renewable energy technologies. Technological development is analysed by through quantitative and qualitative methods. (BA)

77 FR 56681 - Nanoscale Science, Engineering, and Technology Subcommittee; Committee on Technology, National...

Science.gov (United States)

2012-09-13

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering, and Technology...: Notice of webinar. SUMMARY: The National Nanotechnology Coordination Office (NNCO), on behalf of the Nanoscale Science, Engineering, and Technology (NSET) Subcommittee of the Committee on Technology, National...

Marketing technologically advanced products

NARCIS (Netherlands)

Bender, Horst

1989-01-01

This paper calls for a merger of technology and marketing under a customer value perspective; for an enhancement of the traditional technological innovation orientation of the technology-based firm with a market thrust. It establishes technology-based products as product-service offerings that are

7th Annual Science and Engineering Technology Conference/DoD Technology Exposition Volume 1

Science.gov (United States)

2006-04-20

Disruptive Technologies • Army Approach to Disruptive Technologies and Transition Mr. Dennis Schmidt, Director, Science & Technology Integration, Office of...the Assistant Secretary of the Army for Research and Technology • Navy Approach to Disruptive Technologies and Transition Mr. Lewis DeSandre, Program...Manager, ONR 351 • Air Force Approach to Disruptive Technologies and Transition Colonel Mark Stephen, Associate Deputy Assistant Secretary (Science

Examining the Relationship among High-School Teachers' Technology Self-Efficacy, Attitudes towards Technology Integration, and Quality of Technology Integration

Science.gov (United States)

Gonzales, Stacey

2013-01-01

This quantitative study explored the relationships among high-school teachers' (n = 74) technology self-efficacy, teachers' attitudes towards technology integration, and quality of teachers' technology integration into instruction. This study offered the unique perspectives of in-service high-school teachers as they have first-hand experience…

75 FR 39044 - Unisys Corporation, Technology Business Segment, Unisys Information Technology Division, Formerly...

Science.gov (United States)

2010-07-07

..., Technology Business Segment, Unisys Information Technology Division, Formerly Known as BETT, Including... Assistance on April 29, 2010, applicable to workers of Unisys Corporation, Technology Business Segment... employees under the control of the Plymouth, Michigan location of Unisys Corporation, Technology Business...

Superconducting radio frequency technology: Expanding the horizons of physics and technology

International Nuclear Information System (INIS)

Grunder, H.A.; Leemann, C.W.; Sundelin, R.M.; Hartline, B.K.

1986-01-01

This paper describes a major new technology supporting the further evolution of accelerators: superconducting radio frequency (SRF) technology, which is today on the verge of large-scale application in accelerators. Originally foreseen in the early 1960s as a promising technology, SRF only recently has overcome several technological and practical hurdles. SRF accelerating structures promise low rf losses and high gradients under cw operation. High-quality, intense cw beams can be accelerated without risk of melting the structure and without requiring enormous amounts of input rf power

The Western Environmental Technology Office (WETO), Butte, Montana. Technology summary (Revised)

Energy Technology Data Exchange (ETDEWEB)

NONE

1996-03-01

This document has been prepared by the US Department of Energy`s (DOE`s) Office of Environmental Management (EM) Office of Science and Technology (OST) to highlight its research, development, demonstration, testing, and evaluation (RDDT&E) activities funded through the Western environmental Technology Office (WETO) in Butte, Montana. Technologies and processes described in this document have the potential to enhance DOE`s cleanup and waste management efforts, as well as improve US industry`s competitiveness in global environmental markets. The information presented in this document has been assembled from recently produced OST documents that highlight technology development activities within each of the OST program elements and Focus Areas. This document presents one in a series for each of DOE`s Operations Office and Energy Technology Centers.

The Western Environmental Technology Office (WETO), Butte, Montana. Technology summary (Revised)

International Nuclear Information System (INIS)

1996-03-01

This document has been prepared by the US Department of Energy's (DOE's) Office of Environmental Management (EM) Office of Science and Technology (OST) to highlight its research, development, demonstration, testing, and evaluation (RDDT ampersand E) activities funded through the Western environmental Technology Office (WETO) in Butte, Montana. Technologies and processes described in this document have the potential to enhance DOE's cleanup and waste management efforts, as well as improve US industry's competitiveness in global environmental markets. The information presented in this document has been assembled from recently produced OST documents that highlight technology development activities within each of the OST program elements and Focus Areas. This document presents one in a series for each of DOE's Operations Office and Energy Technology Centers

Industrial technology transfer

International Nuclear Information System (INIS)

Bulger, W.

1982-06-01

The transfer of industrial technology is an essential part of the CANDU export marketing program. Potential customers require the opportunity to become self-sufficient in the supply of nuclear plant and equipment in the long term and they require local participation to the maximum extent possible. The Organization of CANDU Industries is working closely with Atomic Energy of Canada Ltd. in developing comprehensive programs for the transfer of manufacturing technology. The objectives of this program are: 1) to make available to the purchasing country all nuclear component manufacturing technology that exists in Canada; and 2) to assure that the transfer of technology takes place in an efficient and effective way. Technology transfer agreements may be in the form of joint ventures or license agreements, depending upon the requirements of the recipient

Making technology public

DEFF Research Database (Denmark)

Winthereik, Brit Ross; Johannsen, Nis; Strand, Dixi Louise

2008-01-01

the transformative potential of the portal presentation for reconfiguring relationships between citizens, health care systems, and information and communication technology (ICT). The analysis is guided by Haraway's notion of diffraction. Findings – The analysis demonstrates the particular way in which the user...... secure the future of the technology and organisation behind it. Research limitations/implications – The paper extends the script metaphor beyond a limited designer-technology-user configuration and argues that scripts in the paraphernalia of technologies also can and should be “de......Purpose – Through an analysis of a demonstration video presenting a new national e-health portal, this paper aims to explore the assumptions and limitations of the concept of “script” and suggests a different approach to analysing the moral order of technology design. Design...

Working around technologies

DEFF Research Database (Denmark)

Dupret, Katia

2017-01-01

in Denmark. The aim and contribution of the study is twofold. First, it attempts to revitalise the discussion on technology workaround strategies as responsible professionalism. Second, it will direct attention to and contribute to an understanding of how the normativity embedded in technological development...... expressions of professionals’ active encounter with the complexity of work situations, and can therefore be important signs of professional ethical judgement. Drawing on science and technology studies and the concept of invisible work, the study discusses workaround situations that arise in health care work......This study discusses how professionalism and work ethics influence how health care professionals work around new technologies. When people avoid using technologies, they are not necessarily ceasing to engage in their work activities. The workaround strategies presented here are rather practical...

Authoritative knowledge, the technological imperative and women's responses to prenatal diagnostic technologies.

Science.gov (United States)

McCoyd, Judith L M

2010-12-01

Theories about authoritative knowledge (AK) and the technological imperative have received varying levels of interest in anthropological, feminist and science and technology studies. Although the anthropological literature abounds with empirical considerations of authoritative knowledge, few have considered both theories through an empirical, inductive lens. Data extracted from an earlier study of 30 women's responses to termination for fetal anomaly are reanalyzed to consider the women's views of, and responses to, prenatal diagnostic technologies (PNDTs). Findings indicate that a small minority embrace the societal portrayal of technology as univalently positive, while the majority have nuanced and ambivalent responses to the use of PNDTs. Further, the interface of authoritative knowledge and the technological imperative suggests that AK derives not only from medical provider status and technology use, but also from the adequacy and trustworthiness of the information. The issue of timing and uncertainty of the information also are interrogated for their impact on women's lives and what that can illuminate about the theories of AK and the technological imperative.

Technology as a factor of identity transformation: development of HOMO TECHNOLOGIES

Directory of Open Access Journals (Sweden)

Vadim A. Emelin

2016-03-01

Full Text Available Based on the understanding of identity as an individual’s identity with the self within the cultural historical chronotope, and experienced as the feeling of belonging/ non-belonging to some communities, controllability/uncontrollability of situations and predictability/non-predictability of events, the paper describes the processes of identity transformation in the course of a technological development. Taken as the initial point, the idea of organ-extension (K. Marx, E. Kapp and technological extension of man (S. Freud, M. McLuhan means that technologies are the extension of a human body and its organs. The processes of technological extension assume a particular scale under condition of information society development, within which computer-, telecommunication-, transport-, bio-, nano- and other high technologies have become an actual cultural historical force that has a power to transform a human. Special attention is paid to the fact that unlike the precedent technologies that have just facilitated some or other human performance, modern technologies of information society do not only change the human topology, widen and expand human natural abilities but also really transform higher mental functions and mediate mental processes and relations between human individuals. Man becomes not only a biological and social creature but also a technological one, i.e. so called HOMO TECHNOLOGICUS. The scale and speed of cultural historical changes make the study of the technological extensions role in the transformation of identity a key point for developing ways of comprehending their role in the life of a modern person, and also for forecasting the evolution of relationship between man and machines in the future.

Advanced Information Technology Investments at the NASA Earth Science Technology Office

Science.gov (United States)

Clune, T.; Seablom, M. S.; Moe, K.

2012-12-01

The NASA Earth Science Technology Office (ESTO) regularly makes investments for nurturing advanced concepts in information technology to enable rapid, low-cost acquisition, processing and visualization of Earth science data in support of future NASA missions and climate change research. In 2012, the National Research Council published a mid-term assessment of the 2007 decadal survey for future spacemissions supporting Earth science and applications [1]. The report stated, "Earth sciences have advanced significantly because of existing observational capabilities and the fruit of past investments, along with advances in data and information systems, computer science, and enabling technologies." The report found that NASA had responded favorably and aggressively to the decadal survey and noted the role of the recent ESTO solicitation for information systems technologies that partnered with the NASA Applied Sciences Program to support the transition into operations. NASA's future missions are key stakeholders for the ESTO technology investments. Also driving these investments is the need for the Agency to properly address questions regarding the prediction, adaptation, and eventual mitigation of climate change. The Earth Science Division has championed interdisciplinary research, recognizing that the Earth must be studied as a complete system in order toaddress key science questions [2]. Information technology investments in the low-mid technology readiness level (TRL) range play a key role in meeting these challenges. ESTO's Advanced Information Systems Technology (AIST) program invests in higher risk / higher reward technologies that solve the most challenging problems of the information processing chain. This includes the space segment, where the information pipeline begins, to the end user, where knowledge is ultimatelyadvanced. The objectives of the program are to reduce the risk, cost, size, and development time of Earth Science space-based and ground

Does internationalisation of technology determine technological diversification in large firms?

OpenAIRE

Christian Le Bas; Pari Patel

2005-01-01

The purpose of the paper is to examine the relationship between technological diversification and internationalisation of technology for large multinational firms, operating at the world technological frontier. More precisely we address the question as to whether internationalisation determines diversification. The analysis is based on a rich database of the European patenting activity of 345 large multinational firms with the highest levels of patenting over two periods of time (1988-1990 an...

Green technology foresight of high technology: a social shaping of technology approach to the analysis of hopes and hypes

DEFF Research Database (Denmark)

Jørgensen, Michael Søgaard; Jørgensen, Ulrik

2009-01-01

Protection Agency with the purpose of acquiring knowledge about the environmental potentials and risks related to the three areas of technology. The foresight was organized with a social shaping of technology (SST) approach to the field in order to cater for the complex relationship between societal demands......, technology options, innovation dynamics and environmental impacts. The approach involved studying actor-networks, laboratory programs and technology trajectories as well as deconstructing different stakeholders’ high tech visions. The identified environmental potentials and risks related to the three areas...

DYNAPHORE, INC., FORAGER SPONGE TECHNOLOGY - INNOVATIVE TECHNOLOGY EVALUATION REPORT

Science.gov (United States)

The Forager Sponge is a volume reduction technology in which heavy metal contaminants from an aqueous medium are selectively concentrated into a smaller volume for facilitated disposal. he technology treats contaminated groundwater, surface voters and porous waters by absorbing d...

Teaching With(out) Technology: Secondary English Teachers and Classroom Technology Use

Science.gov (United States)

Flanagan, Sara; Shoffner, Melanie

2013-01-01

Technology plays an integral role in the English Language Arts (ELA) classroom today, yet teachers and teacher educators continue to develop understandings of how technology influences pedagogy. This qualitative study explored how and why two ELA teachers used different technologies in the secondary English classroom to plan for and deliver…

Legitimation problems of participatory processes in technology assessment and technology policy.

Science.gov (United States)

Saretzki, Thomas

2012-11-01

Since James Carroll (1971) made a strong case for "participatory technology", scientists, engineers, policy-makers and the public at large have seen quite a number of different approaches to design and implement participatory processes in technology assessment and technology policy. As these participatory experiments and practices spread over the last two decades, one could easily get the impression that participation turned from a theoretical normative claim to a working practice that goes without saying. Looking beyond the well-known forerunners and considering the ambivalent experiences that have been made under different conditions in various places, however, the "if" and "how" of participation are still contested issues when questions of technology are on the agenda. Legitimation problems indicate that attempts to justify participation in a given case have not been entirely successful in the eyes of relevant groups among the sponsors, participants, organizers or observers. Legitimation problems of participatory processes in technology assessment and technology policy vary considerably, and they do so not only with the two domains and the ways of their interrelation or the specific features of the participatory processes. If we ask whether or not participation is seen as problematic in technology assessment and technology policy-making and in what sense it is being evaluated as problematic, then we find that the answer depends also on the approaches and criteria that have been used to legitimize or delegitimize the call for a specific design of participation.

High-throughput screening of hybridoma supernatants using multiplexed fluorescent cell barcoding on live cells.

Science.gov (United States)

Lu, Mei; Chan, Brian M; Schow, Peter W; Chang, Wesley S; King, Chadwick T

2017-12-01

With current available assay formats using either immobilized protein (ELISA, enzyme-linked immunosorbent assay) or immunostaining of fixed cells for primary monoclonal antibody (mAb) screening, researchers often fail to identify and characterize antibodies that recognize the native conformation of cell-surface antigens. Therefore, screening using live cells has become an integral and important step contributing to the successful identification of therapeutic antibody candidates. Thus the need for developing high-throughput screening (HTS) technologies using live cells has become a major priority for therapeutic mAb discovery and development. We have developed a novel technique called Multiplexed Fluorescent Cell Barcoding (MFCB), a flow cytometry-based method based upon the Fluorescent Cell Barcoding (FCB) technique and the Luminex fluorescent bead array system, but is applicable to high-through mAb screens on live cells. Using this technique in our system, we can simultaneously identify or characterize the antibody-antigen binding of up to nine unique fluorescent labeled cell populations in the time that it would normally take to process a single population. This has significantly reduced the amount of time needed for the identification of potential lead candidates. This new technology enables investigators to conduct large-scale primary hybridoma screens using flow cytometry. This in turn has allowed us to screen antibodies more efficiently than before and streamline identification and characterization of lead molecules. Copyright © 2017 Elsevier B.V. All rights reserved.

Educational Technology in China

Science.gov (United States)

Meifeng, Liu; Jinjiao, Lv; Cui, Kang

2010-01-01

This paper elaborates the two different academic views of the identity of educational technology in China at the current time--advanced-technology-oriented cognition, known as Electrifying Education, and problem-solving-oriented cognition, known as Educational Technology. It addresses five main modes of educational technology in China: as a…

Ergonomics technology

Science.gov (United States)

Jones, W. L.

1977-01-01

Major areas of research and development in ergonomics technology for space environments are discussed. Attention is given to possible applications of the technology developed by NASA in industrial settings. A group of mass spectrometers for gas analysis capable of fully automatic operation has been developed for atmosphere control on spacecraft; a version for industrial use has been constructed. Advances have been made in personal cooling technology, remote monitoring of medical information, and aerosol particle control. Experience gained by NASA during the design and development of portable life support units has recently been applied to improve breathing equipment used by fire fighters.

Technology in Education

Science.gov (United States)

Roden, Kasi

2011-01-01

This paper was written to support a position on using technology in education. The purpose of this study was to support the use of technology in education by synthesizing previous research. A variety of sources including books and journal articles were studied in order to compile an overview of the benefits of using technology in elementary,…

Avionics systems integration technology

Science.gov (United States)

Stech, George; Williams, James R.

1988-01-01

A very dramatic and continuing explosion in digital electronics technology has been taking place in the last decade. The prudent and timely application of this technology will provide Army aviation the capability to prevail against a numerically superior enemy threat. The Army and NASA have exploited this technology explosion in the development and application of avionics systems integration technology for new and future aviation systems. A few selected Army avionics integration technology base efforts are discussed. Also discussed is the Avionics Integration Research Laboratory (AIRLAB) that NASA has established at Langley for research into the integration and validation of avionics systems, and evaluation of advanced technology in a total systems context.

ACR-700 advanced technologies

International Nuclear Information System (INIS)

Tapping, R.L.; Turner, C.W.; Yu, S.K.W.; Olmstead, R.; Speranzini, R.A.

2004-01-01

A successful advanced reactor plant will have optimized economics including reduced operating and maintenance costs, improved performance, and enhanced safety. Incorporating improvements based on advanced technologies ensures cost, safety and operational competitiveness of the ACR-700. These advanced technologies include modern configuration management; construction technologies; operational technology for the control centre and information systems for plant monitoring and analysis. This paper summarizes the advanced technologies used to achieve construction and operational improvements to enhance plant economic competitiveness, advances in the operational technology used for reactor control, and presents the development of the Smart CANDU suite of tools and its application to existing operating reactors and to the ACR-700. (author)

Competitiveness through cooperation between electricity and information technology. TESLA - Information technology and electric power systems technology programme 1998-2002

Energy Technology Data Exchange (ETDEWEB)

NONE

1998-11-01

The electricity markets are being opened up to competition all round the world. To succeed in competition electricity sellers want new information technology tools to use in managing the sale of electricity. The network companies are aiming to step up utilization of their distribution capacity and to optimize power quality and the reliability of supply. Consumers need solutions with which they can manage their own power consumption and tendering sellers. The Nordic countries have been the first to deregulate their electricity markets. This head start in time is being made use of to generate a head start in technology. Tekes has initiated a technology programme for the years 1998 to 2002, named TESLA - Information Technology and Electric Power Systems, to promote the competitiveness of the Finnish electricity industry in changing conditions. The objective of the programme is to adapt information technology extensively to power distribution and thus develop the potential for Finland`s electricity industry to succeed on world markets. At the moment power distribution technology forms about one third of Finland`s energy technology exports. The programme is also aimed at developing new data transfer and data processing applications for companies in information technology clusters. For Finnish parties in the electricity markets the programme will produce ways and means of (1) improving management and use of distribution networks, (2) implementing competition in electricity sales, and (3) increasing the efficiency of electricity use

Teacher Educator Technology Competencies

Science.gov (United States)

Foulger, Teresa S.; Graziano, Kevin J.; Schmidt-Crawford, Denise A.; Slykhuis, David A.

2017-01-01

The U.S. National Educational Technology Plan recommends the need to have a common set of technology competencies specifically for teacher educators who prepare teacher candidates to teach with technology (U.S. Department of Education, Office of Educational Technology, 2017). This study facilitated the co-creation of the Teacher Educator…

Technology in Education: Technology Integration into the School's Curriculum

Science.gov (United States)

Culver, Bobby L., Jr.

2017-01-01

Integrating technology into the school's curriculum is a very contentious issue. However, it is an important issue that schools need to consider and assess. The purpose of this study was to examine the relationship between K-5th grade teachers' perceptions of proficiency of technology equipment, experience with technology in education, and…

Wasting the Future: The Technological Sublime, Communications Technologies, and E-waste

Directory of Open Access Journals (Sweden)

Sebine Label

2012-08-01

Full Text Available Literally speaking, e-waste is the future of communications. E-waste is the fastest growing waste stream in the world, much of it communications technologies from cell phones to laptops, televisions to peripherals. As a result of policies of planned obsolescence working computers, cell phones, and tablets are routinely trashed. One of the most powerful and enduring discourses associated with emerging technologies is the technological sublime, in which technology is seen as intellectually, emotionally, or spiritually transcendent. It comprises a contradictory impulse that elevates technology with an almost religious fervor, while simultaneously overlooking some of the consequences of industrialism, as well as ignoring the necessity of social, economic, and governmental infrastructures necessary to the implementation and development of new technologies. The idea that a new technology will not pollute or harm the environment is a persistent, though often quickly passed over, theme in the technological sublime, echoed in discourses about emerging technologies such as the silicon chip, the internet, and other ICTs. In this paper, I make connections between the discourse of newness, the practice of planned obsolescence, and the mountains of trashed components and devices globally. Considering the global context demonstrates the realities of the penetration of ICTs and their enduring pollution and negative implications for the health of humans and nonhumans, including plants, animals, waterways, soil, air and so on. I use the discourse of the technological sublime to open up and consider the future of communications, to argue that this discourse not only stays with us but also contains within it two important and related components, the promise of ecological harmony and a future orientation. I argue that these lingering elements keep us from considering the real future of communications – e-waste – and that, as communications scholars, we must also

History of science and technology

Energy Technology Data Exchange (ETDEWEB)

Jang, Byeong Ju

1986-04-15

This book shows origin of technology and development of civilization, origin of science and dissemination of ironware, accumulation of science and technology in the Middle Age society, the era of the Renaissance and science, factory-made manual industry and mechanistic nature view, the era of scientific enlightenment, industrial revolution, science and technology of Korea, formation of modern science and technology, modern technology and approach to science and technology, science and technology in the twenty century such as biochemistry and physics, and cooperation of science and technology.

History of science and technology

International Nuclear Information System (INIS)

Jang, Byeong Ju

1986-04-01

This book shows origin of technology and development of civilization, origin of science and dissemination of ironware, accumulation of science and technology in the Middle Age society, the era of the Renaissance and science, factory-made manual industry and mechanistic nature view, the era of scientific enlightenment, industrial revolution, science and technology of Korea, formation of modern science and technology, modern technology and approach to science and technology, science and technology in the twenty century such as biochemistry and physics, and cooperation of science and technology.

Stimulating R and D of industrial energy-efficient technology. Policy lessons--impulse technology

International Nuclear Information System (INIS)

Luiten, Esther; Blok, Kornelis

2004-01-01

Stimulating research and development (R and D) of innovative energy-efficient technologies for industry is an attractive option for reducing greenhouse gas emissions. Impulse technology, an innovative papermaking technology, is always included in studies assessing the long-term potential of industrial energy efficiency. Aim of this article is to analyse the R and D trajectory of impulse technology in order to explore how government can stimulate the development of industrial energy-efficient technology. The concept of 'momentum' is used to characterise the network of actors and to understand the effect of government R and D support in this particular case study. The network analysis convincingly shows that although marketed as an energy-efficient technology, other benefits were in fact driving forces. Researchers at various national pulp and paper research institutes were successful in attracting government R and D support by claiming an improved energy efficiency. The momentum of the technology network was modest between 1980 and 1990. Therefore, government R and D support accelerated the development of impulse technology in this period. However, when the perspectives of the technology deteriorated--momentum decreased--researchers at national research institutes continued to attract government R and D support successfully. But 25 years of R and D--and over 15 years government R and D support--have not yet resulted in a proven technology. The case study illustrates the risk of continuing R and D support too long without taking into account actors' drivers to invest in R and D. Once momentum decreased, government should have been more circumspect in evaluating the (energy efficiency) promise of impulse technology. The major policy lesson is that government has to look beyond claimed energy efficiencies; government has to value (qualitative) information on (changing) technology networks in deciding upon starting, continuing or pulling out financial R and D support to

Future Information Technology

CERN Document Server

Stojmenovic, Ivan; Choi, Min; Xhafa, Fatos; FutureTech 2013

2014-01-01

Future technology information technology stands for all of continuously evolving and converging information technologies, including digital convergence, multimedia convergence, intelligent applications, embedded systems, mobile and wireless communications, bio-inspired computing, grid and cloud computing, semantic web, user experience and HCI, security and trust computing and so on, for satisfying our ever-changing needs. In past twenty five years or so, Information Technology (IT) influenced and changed every aspect of our lives and our cultures. These proceedings foster the dissemination of state-of-the-art research in all future IT areas, including their models, services, and novel applications associated with their utilization.

Technology for Boundaries

DEFF Research Database (Denmark)

Bødker, Susanne; Kristensen, Jannie Friis; Nielsen, Christina

2003-01-01

.After analysing the history and the current boundary work, the paper will propose new technological support for boundary work. In particular the paper will suggest means of supporting boundaries when these are productive and for changing boundaries when this seems more appropriate. In total, flexible technologies......This paper presents a study of an organisation, which is undergoing a process transforming organisational and technological boundaries. In particular, we shall look at three kinds of boundaries: the work to maintain and change the boundary between the organisation and its customers; boundaries...... seem a core issue when dealing with technology for boundaries....

Globalization and Technology

Directory of Open Access Journals (Sweden)

Traian-Alexandru Miu

2016-01-01

Full Text Available Globalization, very complex phenomenon, involves overcoming the barriers between different states, which allowed the rapid transfer of capital, technology, information, and the "toxins" from one country to another. First, the technology formed the basis of rapid expansion of great ideas promoted by globalization. Undeniable progress in the field of technology and science, has conferred to the man extraordinary powers that have been used most often to the detriment of his spiritual progress. We must not deny that science and technology have brought many benefits to human, and he could expand the knowledge horizon upon the world in which he lives, exploiting information acquired and share them with others. Science and technology must become for postmodern man ways of talk and communion between human and divinity, all to the praise of God and the perfection of the creature.

Democratization of philosophy of technologies

OpenAIRE

Arun Kumar Tripathi

2015-01-01

Technology is a form of culture. Technology is shaping the theoretical framework of our social existence. The technological form of life is part and parcel of culture, just as culture in the human sense inescapably implies technologies. There are unfathomable effects of technology on human culture and society. This paper presents the background and the editorial introduction to the special issue: symposium on Education, Technology, & Democracy: Democratization of Technologies.

Increased Blood Levels of Growth Factors, Proinflammatory Cytokines, and Th17 Cytokines in Patients with Newly Diagnosed Type 1 Diabetes.

Directory of Open Access Journals (Sweden)

Kristi Alnek

Full Text Available The production of several cytokines could be dysregulated in type 1 diabetes (T1D. In particular, the activation of T helper (Th type 1 (Th1 cells has been proposed to underlie the autoimmune pathogenesis of the disease, although roles for inflammatory processes and the Th17 pathway have also been shown. Nevertheless, despite evidence for the role of cytokines before and at the onset of T1D, the corresponding findings are inconsistent across studies. Moreover, conflicting data exist regarding the blood cytokine levels in T1D patients. The current study was performed to investigate genetic and autoantibody markers in association with the peripheral blood cytokine profiles by xMap multiplex technology in newly diagnosed young T1D patients and age-matched healthy controls. The onset of young-age T1D was characterized by the upregulation of growth factors, including granulocyte macrophage-colony stimulating factor (GM-CSF and interleukin (IL-7, the proinflammatory cytokine IL-1β (but not IL-6 or tumor necrosis factor [TNF]-α, Th17 cytokines, and the regulatory cytokines IL-10 and IL-27. Ketoacidosis and autoantibodies (anti-IA-2 and -ZnT8, but not human leukocyte antigen (HLA genotype, influenced the blood cytokine levels. These findings broaden the current understanding of the dysregulation of systemic levels of several key cytokines at the young-age onset of T1D and provide a further basis for the development of novel immunoregulatory treatments in this disease.

VASCULAR ENDOTHELIAL GROWTH FACTORS IN HEART TRANSPLANT REJECTIONS

Directory of Open Access Journals (Sweden)

O. P. Shevchenko

2015-01-01

Full Text Available Aim: to determine the clinical significance of vascular endothelial growth factors VEGF-A, VEGF-D, PlGF-1 to assess the risk of cardiovascular complications in heart recipients. Materials and methods. 103 patients, aged 16 to 73 years, 85 males and 18 females. 65 recipients (47 men and 18 women had dilated cardiomyopathy, 38 – coronary heart disease (CHD. The concentration of VEGF-A, VEGF-D, PlGF-1 was measured using xMAP technology with sets of reagents Simplex ProcartaPlex™. Results. After HTx the level of VEGF-A significantly decreased, p = 0.001. There were no correlations between the levels of VEGF-A, VEGF-D and PlGF-1 with age, gender and diagnosis. After HTx VEGF-A level was higher in recipients with ACR than in those without it (p = 0.001. ACR frequency was significantly higher in patients with high VEGF-A level (≥316.5 pg/ml, RR = 5.8 ± 0.5, AUC = 0.779. After HTx PlGF-1 level was higher in recipients with ACR too (p = 0.039. ACR frequency was significantly higher in patients with high PlGF-1 level (≥5.33 pg/ml, RR = 1.8 ± 0.5, AUC = 0.65. There were no correlations between VEGF-D level with ACR and all three biomarkers with AMR. ACR frequency was significantly higher with both high VEGF-A and PlGF-1 levels (RR = 6.4. Conclusion. Serum levels of VEGF-A and PlGF-1 after HTx may be regarded as indicators of increased risk of ACR.

Newnes communications technology handbook

CERN Document Server

Lewis, Geoff

1994-01-01

Newnes Communications Technology Handbook provides a discussion on different topics relevant to communications technology. The book is comprised of 39 chapters that tackle a wide variety of concern in communications technology. The coverage of the text includes technologies, such as analog digital communications systems, radio frequency receiver, and satellite systems. The book also discusses some methods and techniques used in communications technology, including mixer signal processing, modulation and demodulation, and spread spectrum techniques. The text will be of great use to engineers, t

Technology Empowerment: Security Challenges.

Energy Technology Data Exchange (ETDEWEB)

Warren, Drake Edward [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Backus, George A. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Jones, Wendell [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Nelson, Thomas R. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Skocypec, Russell D. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

2017-03-01

“Technology empowerment” means that innovation is increasingly accessible to ordinary people of limited means. As powerful technologies become more affordable and accessible, and as people are increasingly connected around the world, ordinary people are empowered to participate in the process of innovation and share the fruits of collaborative innovation. This annotated briefing describes technology empowerment and focuses on how empowerment may create challenges to U.S. national security. U.S. defense research as a share of global innovation has dwindled in recent years. With technology empowerment, the role of U.S. defense research is likely to shrink even further while technology empowerment will continue to increase the speed of innovation. To avoid falling too far behind potential technology threats to U.S. national security, U.S. national security institutions will need to adopt many of the tools of technology empowerment.

Technology Museums in Denmark

DEFF Research Database (Denmark)

Søndergaard, Morten Karnøe

2012-01-01

This article presents an exhibit review of some of the major technology museums in Denmark. First comes an introduction to the Danish museum ”landscape”. Second a total of six museums and their technology focused exhibits are presented. Among the museums are the Fisheries and Maritime Museum...... in Esbjerg, housing one of the most impressive and representative exhibitions on the technology behind the strong Danish maritime sector. Another museum being mentioned is the Energy Museum, which covers the background for some of the major breakthroughs performed in Denmark within this area; particularly...... within wind power technology. Finally special attention is devoted to the Danish Technological Museum. A museum which is the oldest and most elaborate of all the technology museums. The museum covers virtually every technological breakthrough with any relevance in a Danish section, with a special focus...

Bridging the Gap: Technology Trends and Use of Technology in Schools

Science.gov (United States)

Lim, Cher Ping; Zhao, Yong; Tondeur, Jo; Chai, Ching Sing; Tsai, Chin-Chung

2013-01-01

Considerable investment has been made to bring technology to schools and these investments have indeed resulted in many "success stories." However there are two significant gaps in educational uses of technology that must be addressed. The first is a usage gap. Compared to how and how much today's students use technology outside…

Nuclear technology and societal needs

International Nuclear Information System (INIS)

2004-11-01

This volume aims to review the present status of development of nuclear technologies and their applications in the country and also to make projections for future requirements. This will also cover state-of-the-art technologies in these areas. The following topics are covered in detail: nuclear technologies for water desalination, water resources development and management using nuclear technology, industrial applications of isotopes and radiation technology, radiation technology in health care, nuclear technology for food preservation, agricultural applications of nuclear technology. Papers relevant to INIS are indexed separately

Basic Principle of Advanced Oxidation Technology : Hybrid Technology Based on Ozone and Titania

International Nuclear Information System (INIS)

Widdi Usada; Agus Purwadi

2007-01-01

One of problems in health environment is organic liquid waste from many pollutant resources. Environmental friendly technology for degrading this waste is ozone which produced by plasma discharge technology, but its capability is limited. However, it is needed a new environmental friendly technology which has stronger capability. This new technology is so called advanced oxidation technology. Advanced oxidation technology is a hybrid of ozone, peroxide, UV light and photo catalyst. In this paper, it is introduced basic principle of hybrid of ozone and titania photo catalyst semiconductor. The capability of organic liquid degradation will be stronger because there is new radical which is produced by chemical reaction between electron-hole pair from photo catalyst titania and water or oxygen. This new radical then degrades this organic pollutant. This technology is used to degrade phenol. (author)

Advanced Manufacturing Technologies

Science.gov (United States)

Fikes, John

2016-01-01

Advanced Manufacturing Technologies (AMT) is developing and maturing innovative and advanced manufacturing technologies that will enable more capable and lower-cost spacecraft, launch vehicles and infrastructure to enable exploration missions. The technologies will utilize cutting edge materials and emerging capabilities including metallic processes, additive manufacturing, composites, and digital manufacturing. The AMT project supports the National Manufacturing Initiative involving collaboration with other government agencies.

Materials and technology

International Nuclear Information System (INIS)

Gockel, E.; Simon, J.

1998-01-01

New materials and the processes for their economical fabrication and use are the factors which drive innovation in totally different fields of technology, such as energy engineering, transport, and information. But they also open up new fields of technology such as micro systems or medicine technology. Five out of a total of twelve articles are separately listed in the ENERGY database [de

Information Technology Resources Assessment

Energy Technology Data Exchange (ETDEWEB)

1993-04-01

The Information Technology Resources Assessment (ITRA) is being published as a companion document to the Department of Energy (DOE) FY 1994--FY 1998 Information Resources Management Long-Range Plan. This document represents a collaborative effort between the Office of Information Resources Management and the Office of Energy Research that was undertaken to achieve, in part, the Technology Strategic Objective of IRM Vision 21. An integral part of this objective, technology forecasting provides an understanding of the information technology horizon and presents a perspective and focus on technologies of particular interest to DOE program activities. Specifically, this document provides site planners with an overview of the status and use of new information technology for their planning consideration.

The Impact of Technological and Non-Technological Innovations on Firm Growth

OpenAIRE

Ali-Yrkkö, Jyrki; Martikainen, Olli

2008-01-01

This study investigates the relationship between innovations and firm growth, based on the data of Finnish firms operating in the software industry. We find that in terms of turnover and employment, firms with only technological innovations do not grow more rapidly than other firms. However, firm growth is positively associated with the combination of technological and non-technological innovations.

Application study of nuclear technologies for integration chemical, biological and radiological technology

Energy Technology Data Exchange (ETDEWEB)

Shin, Jae Kon; Han, M. H.; Kim, Y. H.; Yang, J. E.; Jung, K. S.; Cha, H. K.; Moon, J.; La, K. H

2001-02-01

The projects are suggested the method to maximize the technology and research results which are being carried out by KAERI on the nuclear field. The study presents 1)the technology to rapidly and accurately determine and the nature of contamination, 2) the technology to predict the spread of contaminant and the magnitude of damage, and 3) the expert-aided decision making technology to identify the optimum counter-measures. And the solutions are also suggested the application to military technology in Chemical, Biological and Radiation field. In addition, I hope this kind of cooperation model come to be the good case of military civilian research harmony to improve the national competition capability.

Educational technology in medical education.

Science.gov (United States)

Han, Heeyoung; Resch, David S; Kovach, Regina A

2013-01-01

This article aims to review the past practices of educational technology and envision future directions for medical education. The discussion starts with a historical review of definitions and perspectives of educational technology, in which the authors propose that educators adopt a broader process-oriented understanding of educational technology. Future directions of e-learning, simulation, and health information technology are discussed based on a systems view of the technological process. As new technologies continue to arise, this process-oriented understanding and outcome-based expectations of educational technology should be embraced. With this view, educational technology should be valued in terms of how well the technological process informs and facilitates learning, and the acquisition and maintenance of clinical expertise.

The Next Technology Revolution - Nano Electronic Technology

Science.gov (United States)

Turlik, Iwona

2004-03-01

Nanotechnology is a revolutionary engine that will engender enormous changes in a vast majority of today's industries and markets, while potentially creating whole new industries. The impact of nanotechnology is particularly significant in the electronics industry, which is constantly driven by the need for higher performance, increased functionality, smaller size and lower cost. Nanotechnology can influence many of the hundreds of components that are typically assembled to manufacture modern electronic devices. Motorola manufactures electronics for a wide range of industries and communication products. In this presentation, the typical components of a cellular phone are outlined and technology requirements for future products, the customer benefits, and the potential impact of nanotechnology on many of the components are discussed. Technology needs include reliable materials supply, processes for high volume production, experimental and simulation tools, etc. For example, even routine procedures such as failure characterization may require the development of new tools for investigating nano-scale phenomena. Business needs include the development of an effective, high volume supply chain for nano-materials and devices, disruptive product platforms, and visible performance impact on the end consumer. An equally significant long-term industry need is the availability of science and engineering graduates with a multidisciplinary focus and a deep understanding of the fundamentals of nano-technology, that can harness the technology to create revolutionary products.

Clinical cytometry and progress in HLA antibody detection.

Science.gov (United States)

Bray, Robert A; Tarsitani, Christine; Gebel, Howard M; Lee, Jar-How

2011-01-01

For most solid organ and selected stem cell transplants, antibodies against mismatched HLA antigens can lead to early and late graft failure. In recognition of the clinical significance of these antibodies, HLA antibody identification is one of the most critical functions of histocompatibility laboratories. Early methods employed cumbersome and insensitive complement-dependent cytotoxicity assays with a visual read-out. A little over 20 years ago flow cytometry entered the realm of antibody detection with the introduction of the flow cytometric crossmatch. Cytometry's increased sensitivity and objectivity quickly earned it popularity as a preferred crossmatch method especially for sensitized recipients. Although a sensitive method, the flow crossmatch was criticized as being "too sensitive" as false positive reactions were a know drawback. In part, the shortcomings of the flow crossmatch were due to the lack of corresponding sensitive and specific HLA antibody screening assays. However, in the mid 1990s, solid phase assays, capable of utilizing standard flow cytometers, were developed. These assays used microparticles coated with purified HLA molecules. Hence, the era of solid-phase, microparticle technology for HLA antibody detection was born permitting the sensitive and specific detection of HLA antibody. It was now possible to provide better correlation between HLA antibody detection and the flow cytometric crossmatch. This flow-based technology was soon followed by adaptation to the Luminex platform permitting a mutltiplexed approach for the identification and characterization of HLA antibodies. It is hoped that these technologies will ultimately lead to the identification of parameters that best correlate with and/or predict transplant outcomes. Copyright © 2011 Elsevier Inc. All rights reserved.

Communication technology update and fundamentals

CERN Document Server

Grant, August E

2010-01-01

New communication technologies are being introduced at an astonishing rate. Making sense of these technologies is increasingly difficult. Communication Technology Update and Fundamentals is the single best source for the latest developments, trends, and issues in communication technology. Featuring the fundamental framework along with the history and background of communication technologies, Communication Technology Update and Fundamentals, 12th edition helps you stay ahead of these ever-changing and emerging technologies.As always, every chapter ha

White paper on future technologies

Energy Technology Data Exchange (ETDEWEB)

NONE

2012-12-15

This book describes the role of technology and challenge of future like why we focus on future technologies and future, human being and technology, methodology on development for future technologies such as global monitoring system for investigation on environmental change, investigation of research front for paper and patent and COMPAS, and domestic and foreign organization for discover on future technologies. It also introduces KISTI selection future technologies 500 : healthy society, smart society, safety society, and future technologies 500.

Does Technology Acceptance Affect E-Learning in a Non-Technology-Intensive Course?

Science.gov (United States)

Buche, Mari W.; Davis, Larry R.; Vician, Chelley

2012-01-01

Prior research suggests that individuals' technology acceptance levels may affect their work and learning performance outcomes when activities are conducted through information technology usage. Most previous research investigating the relationship between individual attitudes towards technology and learning has been conducted in…

Technology Management within Product Lines in High Technology Markets

Science.gov (United States)

Sarangee, Kumar R.

2009-01-01

Understanding the nuances of product line management has been of great interest to business scholars and practitioners. This assumes greater significance for firms conducting business in technologically dynamic industries, where they face certain challenges regarding the management of multiple, overlapping technologies within their product lines.…

Technology Transformation

Science.gov (United States)

Scott, Heather; McGilll, Toria

2011-01-01

Social networking and other technologies, if used judiciously, present the means to integrate 21st century skills into the classroom curriculum. But they also introduce challenges that educators must overcome. Increased concerns about plagiarism and access to technology can test educators' creativity and school resources. Air Academy High School,…

Mars Technology Program Planetary Protection Technology Development

Science.gov (United States)

Lin, Ying

2006-01-01

The objectives of the NASA Planetary Protection program are to preserve biological and organic conditions of solar-system bodies for future scientific exploration and to protect the Earth from potential hazardous extraterrestrial contamination. As the exploration of solar system continues, NASA remains committed to the implementation of planetary protection policy and regulations. To fulfill this commitment, the Mars Technology Program (MTP) has invested in a portfolio of tasks for developing necessary technologies to meet planetary protection requirements for the next decade missions.

Advanced PWR technology development -Development of advanced PWR system analysis technology-

Energy Technology Data Exchange (ETDEWEB)

Jang, Moon Heui; Hwang, Yung Dong; Kim, Sung Oh; Yoon, Joo Hyun; Jung, Bub Dong; Choi, Chul Jin; Lee, Yung Jin; Song, Jin Hoh [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

1995-07-01

The primary scope of this study is to establish the analysis technology for the advanced reactor designed on the basis of the passive and inherent safety concepts. This study is extended to the application of these technology to the safety analysis of the passive reactor. The study was performed for the small and medium sized reactor and the large sized reactor by focusing on the development of the analysis technology for the passive components. Among the identified concepts the once-through steam generator, the natural circulation of the integral reactor, heat pipe for containment cooling, and hydraulic valve were selected as the high priority items to be developed and the related studies are being performed for these items. For the large sized passive reactor, the study plans to extend the applicability of the best estimate computer code RELAP5/MOD3 which is widely used for the safety analyses of the reactor system. The improvement and supplementation study of the analysis modeling and the methodology is planned to be carried out for these purpose. The newly developed technologies are expected to be applied to the domestic advanced reactor design and analysis and these technologies will play a key role in extending the domestic nuclear base technology and consolidating self-reliance in the essential nuclear technology. 72 figs, 15 tabs, 124 refs. (Author).

Applied Semantic Web Technologies

CERN Document Server

Sugumaran, Vijayan

2011-01-01

The rapid advancement of semantic web technologies, along with the fact that they are at various levels of maturity, has left many practitioners confused about the current state of these technologies. Focusing on the most mature technologies, Applied Semantic Web Technologies integrates theory with case studies to illustrate the history, current state, and future direction of the semantic web. It maintains an emphasis on real-world applications and examines the technical and practical issues related to the use of semantic technologies in intelligent information management. The book starts with

Encouraging environmentally strategic technologies

International Nuclear Information System (INIS)

Heaton, G.R.

1994-01-01

Having moved beyond its initial absorption with controlling new technology, environmental policy today must focus more strongly on promoting the development and adoption of new technologies. World Resource Institute's (WRI) ongoing study of 'environmentally strategic technology' is addressed to this fundamental policy issue. The study proposes criteria for identifying such technology, offers a specific list, suggests the kinds of public policy changes necessary to encourage their development and finally presents a comparison of critical technology lists (from the White House, the European Community, Japan and the US Department of Defense). (TEC)

STREAMS - Technology Programme. Yearbook 2003

International Nuclear Information System (INIS)

2003-01-01

The STREAMS Technology Programme addresses municipal waste. Municipal waste is composed of waste from households and small businesses. The programme focuses on five areas Waste prevention, Collection, transportation, and management of waste streams, Waste treatment technologies, Waste recycling into raw materials and new products, Landfill technologies. The development projects of the STREAMS Programme utilize a number of different technologies, such as biotechnology, information technology, materials technology, measurement and analysis, and automation technology. Finnish expertise in materials recycling technologies and related electronics and information technology is extremely high on a worldwide scale even though the companies represent SMEs. Started in 2001, the STREAMS programme has a total volume of 27 million euros, half of which is funded by Tekes. The programme runs through the end of 2004. (author)

Plasma proteins predict conversion to dementia from prodromal disease.

Science.gov (United States)

Hye, Abdul; Riddoch-Contreras, Joanna; Baird, Alison L; Ashton, Nicholas J; Bazenet, Chantal; Leung, Rufina; Westman, Eric; Simmons, Andrew; Dobson, Richard; Sattlecker, Martina; Lupton, Michelle; Lunnon, Katie; Keohane, Aoife; Ward, Malcolm; Pike, Ian; Zucht, Hans Dieter; Pepin, Danielle; Zheng, Wei; Tunnicliffe, Alan; Richardson, Jill; Gauthier, Serge; Soininen, Hilkka; Kłoszewska, Iwona; Mecocci, Patrizia; Tsolaki, Magda; Vellas, Bruno; Lovestone, Simon

2014-11-01

The study aimed to validate previously discovered plasma biomarkers associated with AD, using a design based on imaging measures as surrogate for disease severity and assess their prognostic value in predicting conversion to dementia. Three multicenter cohorts of cognitively healthy elderly, mild cognitive impairment (MCI), and AD participants with standardized clinical assessments and structural neuroimaging measures were used. Twenty-six candidate proteins were quantified in 1148 subjects using multiplex (xMAP) assays. Sixteen proteins correlated with disease severity and cognitive decline. Strongest associations were in the MCI group with a panel of 10 proteins predicting progression to AD (accuracy 87%, sensitivity 85%, and specificity 88%). We have identified 10 plasma proteins strongly associated with disease severity and disease progression. Such markers may be useful for patient selection for clinical trials and assessment of patients with predisease subjective memory complaints. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

Marketing technology in macroeconomics.

Science.gov (United States)

Tamegawa, Kenichi

2012-01-01

In this paper, we incorporate a marketing technology into a dynamic stochastic general equilibrium model by assuming a matching friction for consumption. An improvement in matching can be interpreted as an increase in matching technology, which we call marketing technology because of similar properties. Using a simulation analysis, we confirm that a positive matching technology shock can increase output and consumption.

Emerging technology and ethics

CERN Document Server

Wakunuma, Kutoma

2011-01-01

This e-book on Emerging Technologies and Ethics includes a collection of essays which explore the future and ethics of emerging information and communication technologies. Articles in the collection include an overview of the legal implications which may be relevant to the ethical aspects of emerging technologies and also ethical issues arising from the mass-take up of mobile technologies.

Changes in intramuscular cytokine levels during masseter inflammation in male and female rats

OpenAIRE

Niu, Katelyn Y.; Ro, Jin Y.

2010-01-01

The present study was conducted to examine cytokine profiles in the masseter muscle before and after complete Freund’s adjuvant (CFA)-induced inflammation and possible sex differences in the cytokine levels. Age matched male and female Sprague Dawley rats were injected with CFA in the mid-region of the masseter muscle. Muscle tissue surrounding the injection site was extracted 6 hrs, 1, 3 and 7 days after the injection to measure TNF-α, IL-1β, IL-6 and IL-4 levels with Luminex multi-analyte p...

Technology transfer of brain-computer interfaces as assistive technology: barriers and opportunities.

Science.gov (United States)

Nijboer, F

2015-02-01

This paper provides an analysis of perspectives from different stakeholders on the state-of-the-art of BCI. Three barriers for technology transfer of BCIs as access technologies are identified. First, BCIs are developed with a narrow focus on creating a reliable technology, while a broader focus on creating a usable technology is needed. Second, the potential target group, which could benefit from BCIs as access technologies is expected to be very small. Development costs are therefore high, while reimbursements are expected to be low, which challenges the commercial viability. Third, potential target users should be much more included in the design process of BCIs to ensure that the end-products meet technical, ethical, legal and social requirements. These three issues need to be urgently addressed so that target users may benefit from this promising technology. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

Technology transfer - the role of AEA Technology

International Nuclear Information System (INIS)

Hughes, A.E.; Bullough, R.; Mason, J.P.

1989-01-01

This paper concentrates mostly on examples of spin offs which have arisen from the more basic research carried out by the AEA. However, it should not be inferred from this that the only examples of successful technology transfer by the AEA are of a similar, often unforeseen nature. The most outstanding example of technology transfer by the AEA must surely be that achieved through the applied research which has enabled the establishment of a successful civil nuclear power programme in the UK. The natural transfer of technology here, achieved by virtue of the unique bridging position of the AEA with respect to universities and the nuclear industry, means that its success can easily be overlooked; to do so would be a mistake. However, by including spin off examples, we hope to illustrate how the AEA has also succeeded in bridging to more difficult areas where the special relationship which it shares with the nuclear industry is absent. (author)

Key factors affecting the deployment of electricity generation technologies in energy technology scenarios

International Nuclear Information System (INIS)

Ruoss, F.; Turton, H.; Hirschberg, S.

2009-12-01

This report presents the findings of a survey of key factors affecting the deployment of electricity generation technologies in selected energy scenarios. The assumptions and results of scenarios, and the different models used in their construction, are compared. Particular attention is given to technology assumptions, such as investment cost or capacity factors, and their impact on technology deployment. We conclude that the deployment of available technologies, i.e. their market shares, can only be explained from a holistic perspective, and that there are strong interactions between driving forces and competing technology options within a certain scenario. Already the design of a scenario analysis has important impacts on the deployment of technologies: the choice of the set of available technologies, the modeling approach and the definition of the storylines determine the outcome. Furthermore, the quantification of these storylines into input parameters and cost assumptions drives technology deployment, even though differences across the scenarios in cost assumptions are not observed to account for many of the observed differences in electricity technology deployment. The deployment can only be understood after a consideration of the interplay of technology options and the scale of technology deployment, which is determined by economic growth, end-use efficiency, and electrification. Some input parameters are of particular importance for certain technologies: CO 2 prices, fuel prices and the availability of carbon capture and storage appear to be crucial for the deployment of fossil-fueled power plants; maximum construction rates and safety concerns determine the market share of nuclear power; the availability of suitable sites represents the most important factor for electricity generation from hydro and wind power plants; and technology breakthroughs are needed for solar photovoltaics to become cost-competitive. Finally, this analysis concludes with a review

Key factors affecting the deployment of electricity generation technologies in energy technology scenarios

Energy Technology Data Exchange (ETDEWEB)

Ruoss, F.; Turton, H.; Hirschberg, S.

2009-12-15

This report presents the findings of a survey of key factors affecting the deployment of electricity generation technologies in selected energy scenarios. The assumptions and results of scenarios, and the different models used in their construction, are compared. Particular attention is given to technology assumptions, such as investment cost or capacity factors, and their impact on technology deployment. We conclude that the deployment of available technologies, i.e. their market shares, can only be explained from a holistic perspective, and that there are strong interactions between driving forces and competing technology options within a certain scenario. Already the design of a scenario analysis has important impacts on the deployment of technologies: the choice of the set of available technologies, the modeling approach and the definition of the storylines determine the outcome. Furthermore, the quantification of these storylines into input parameters and cost assumptions drives technology deployment, even though differences across the scenarios in cost assumptions are not observed to account for many of the observed differences in electricity technology deployment. The deployment can only be understood after a consideration of the interplay of technology options and the scale of technology deployment, which is determined by economic growth, end-use efficiency, and electrification. Some input parameters are of particular importance for certain technologies: CO{sub 2} prices, fuel prices and the availability of carbon capture and storage appear to be crucial for the deployment of fossil-fueled power plants; maximum construction rates and safety concerns determine the market share of nuclear power; the availability of suitable sites represents the most important factor for electricity generation from hydro and wind power plants; and technology breakthroughs are needed for solar photovoltaics to become cost-competitive. Finally, this analysis concludes with a

Beliefs, acceptances and technological knowledge

NARCIS (Netherlands)

Vries, de M.J.; Meijers, A.W.M.; Vries, de M.J.; Hansson, S.O.; Meijers, A.W.M.

2013-01-01

One of the four ways of conceptualizing technology that Carl Mitcham distinguished in his book Thinking Through Technology is technology as knowledge. His description of technology as knowledge showed that not much philosophical literature on the nature of technological knowledge was available at

Climbing Up the Technology Ladder? High-Technology Exports in China and Latin America

OpenAIRE

Gallagher, Kevin P.; Porzecanski, Roberto

2008-01-01

In this paper we determine the “dynamic revealed competitiveness position” (DRCP) of nations for high technology exports between 1980 and 2005. We find that the developed world has lost significant market share in high technology and that China has climbed the high technology ladder during this period. In 1980 China was ranked 99th of all nations in terms of the percentage of global exports in high technology. By 2005 China climbed to second place in the world, first place if high technology ...

HEMISPHERIC CENTER FOR ENVIRONMENTAL TECHNOLOGY

Energy Technology Data Exchange (ETDEWEB)

M.A. Ebadian

1999-10-31

The Deactivation and Decommissioning (D&D) Technology Assessment Program (TAP) was developed to provide detailed, comparable data for environmental technologies and to disseminate this data to D&D professionals in a manner that will facilitate the review and selection of technologies to perform decontamination and decommissioning. The objectives for this project include the following: Determine technology needs through review of the Site Technology Coordination Group (STCG) information and other applicable websites and needs databases; Perform a detailed review of industries that perform similar activities as those required in D&D operations to identify additional technologies; Define the technology assessment program for characterization and waste management problem sets; Define the data management program for characterization, dismantlement, and waste management problem sets; Evaluate baseline and innovative technologies under standard test conditions at Florida International University's Hemispheric Center for Environmental Technology (FIU-HCET) and other locations and collect data in the areas of performance, cost, health and safety, operations and maintenance, and primary and secondary waste generation; Continue to locate, verify, and incorporate technology performance data from other sources into the multimedia information system; and Develop the conceptual design for a dismantlement technology decision analysis tool for dismantlement technologies.

Higher education technological knowledge and patterns of technology adoptions in undergraduate STEM courses

Science.gov (United States)

Ali, Zarka Asghar

Identifying, examining, and understanding faculty members' technological knowledge development and the process of technology adoption in higher education is a multifaceted process. Past studies have used Rogers (1995, 2003) diffusion of innovation theoretical framework to delineate the technology adoption process. These studies, however, have frequently reported the influencing factors based on the statistical analysis such as regression analysis-based approach, and have not focused on the emerging process of technology adoptions or the developing process of technological knowledge and pedagogical knowledge. A mixed method study was designed to see how faculty members acquire different technologies and develop technological knowledge that might help them adopt technologies in their classrooms and online using different pedagogies. A sample of STEM teaching faculty members with different ranks, tenure, teaching experience, and varied degree of experience in the use of educational technologies participated in the study. A survey was designed to identify internal and external factors affecting technology adoption and its effective use in different teaching activities. To elaborate survey results, the study also included class observations as well as pre- and post-observation interviews. Online classrooms used by the faculty via Blackboard learning management system, online flipped classrooms, or other websites such as Piazza were also examined for data triangulation. The findings of the study indicate that faculty members are influenced by their own professional motivations and student learning to improve their teaching methods and to enhance student interactions and learning through the use of different educational technologies. The adoption process was identified as spreading over a period of time and it looked at how faculty members' developed their technological knowledge and pedagogical knowledge. With the recognition of the social, organizational, and

Technological learning for carbon capture and sequestration technologies

International Nuclear Information System (INIS)

Riahi, Keywan; Rubin, Edward S.; Taylor, Margaret R.; Schrattenholzer, Leo; Hounshell, David

2004-01-01

This paper analyzes potentials of carbon capture and sequestration technologies (CCT) in a set of long-term energy-economic-environmental scenarios based on alternative assumptions for technological progress of CCT. In order to get a reasonable guide to future technological progress in managing CO 2 emissions, we review past experience in controlling sulfur dioxide (SO 2 ) emissions from power plants. By doing so, we quantify a 'learning curve' for CCT, which describes the relationship between the improvement of costs due to accumulation of experience in CCT construction. We incorporate the learning curve into the energy-modeling framework MESSAGE-MACRO and develop greenhouse gas emissions scenarios of economic, demographic, and energy demand development, where alternative policy cases lead to the stabilization of atmospheric CO 2 concentrations at 550 parts per million by volume (ppmv) by the end of the 21st century. We quantify three types of contributors to the carbon emissions mitigation: (1) demand reductions due to the increased price of energy, (2) fuel switching primarily away from coal, and (3) carbon capture and sequestration from fossil fuels. Due to the assumed technological learning, costs of the emissions reduction for CCT drop rapidly and in parallel with the massive introduction of CCT on the global scale. Compared to scenarios based on static cost assumptions for CCT, the contribution of carbon sequestration is about 50% higher in the case of learning, resulting in cumulative sequestration of CO 2 ranging from 150 to 250 billion (10 9 ) tons with carbon during the 21st century. Also, carbon values (tax) across scenarios (to meet the 550 ppmv carbon concentration constraint) are between 2% and 10% lower in the case of learning for CCT by 2100. The results illustrate that assumptions on technological change are a critical determinant of future characteristics of the energy system, indicating the importance of long-term technology policies in

Development of Korea telecommunication technology

International Nuclear Information System (INIS)

1992-06-01

It concentrates on development of Korea telecommunication technology, which is made up seven chapters. It gives description of manual central telephone exchange or private automatic telephone exchange, transmission technology on wire line and cable line technology and optical transmission, radio communication technology on mobile and natural satellite communication, network technology with intelligent network, broadband ISDN and packet switched Data Network, terminal technology with telephone and data communication terminal and development of Information Technology in Korea. It has an appendix about development of military communication system.

Technologies for a sustainable development; Technologies pour un developpement durable

Energy Technology Data Exchange (ETDEWEB)

NONE

2002-07-01

The European Event on Technology (EET), a recurrent annual event since 1992, is a major meeting opportunity for researchers and engineers as well as private and public decision-makers, on technologies, their evolution and their industrial and social implications. In less than a decade, sustainable development has become both an economic and a political priority. It was urgent and legitimate that those who are the mainsprings should take hold of the subject and give it technological content, estimate its costs and define clear timetables. The debates consist of: plenary sessions on environmental, social and economic stakes of sustainable development and the challenges for, and commitment of engineers, managers and politicians with respect to these goals; and workshops, which provide an overview of recently acquired or upcoming technologies developed by sector: energy, transports, new information technologies, new industrial manufacturing technologies (materials, products, services), waste management, global environment monitoring, water management, bio-technologies, and innovation management. This document brings together the different talks given by the participants. Among these, the following ones fall into the energy and environment scope: energy efficiency of buildings: towards energy autonomy; superconductors enable in new millennium for electric power industry; advanced gas micro-turbine-driven generator technology; environmental and technical challenges of an offshore wind farm; future nuclear energy systems; modelling combustion in engines: progress and prospects for reducing emissions; on-board computers: reduction in consumption and emissions of engine-transmission units for vehicles; polymer-lithium batteries: perspectives for zero-emission traction; hybrid vehicles and energy/environmental optimization: paths and opportunities; fuel cells and zero-emission: perspectives and developments; global change: causes, modeling and economic issues; the GMES

Development of National Technology Audit Policy

Directory of Open Access Journals (Sweden)

Subiyanto Subiyanto

2017-07-01

Full Text Available The Laws have mandated implementation of technology audit, nevertheless such implementation needs an additional policy that is more technical. The concept of national audit technology policy shall make technology audit as a tool to ensure the benefit of technology application for society and technology advance for nation independency. This article discusses on technology audit policy concept especially infrastructure requirement, with emphasis on regulation, implementation tools, and related institution. The development of technology audit policy for national interest requires provision of mandatory audit implementation, accompanied by tools for developing technology auditor’s competence and technology audit institutional’s mechanism. To guide technology auditor’s competence, concept of national audit technology policy shall classify object of technology audit into product technology, production technology, and management of technology, accompanied by related parameters of technology performance evaluation.

The dual role of external technology sourcing in technological exploration

DEFF Research Database (Denmark)

Vanhaverbeke, Wim; Li-Ying, Jason; van de Vrande, Vareska

2013-01-01

from non-partners, partners may play a role because of whom they know. That is, they inform the firm about technological opportunities beyond its corporate venturing network. The empirical analysis supports the dual role of venturing partners in facilitating the two types of explorative learning.......We refine the concept of boundary-spanning exploration, by making a distinction between explorative learning from partners and from non-partners (Partners are organizations with whom a focal firm has some kind of external venturing relations, i.e. technological alliances, corporate venturing...... capital, or M&As). These partners play a dual role: in explorative learning from partners, a firm teams up with external venturing partners to co-develop or transfer technology. Partners’ technology base (what they know) is driving explorative learning from partners. In contrast, in explorative learning...

INFORMATION TECHNOLOGY, INTERNET, AND MARKETING

OpenAIRE

Mihane Berisha-Namani

2013-01-01

Information technology developed very fast, and today's marketing activities are not possible without the help of information technology. Furthermore, it is difficult to think of a policy domain not affected by usage of information technology. Information technology is making possible and creating connections between businesses and organizations. The implications of information technology usage in marketing activities are profound too. Using information technology, companies possess the poten...

From Technology Teacher to Technology Integration Specialist: Preparing for a Paradigm Shift

Science.gov (United States)

Dalrymple, Jennifer Lynn Penry

2017-01-01

This dissertation examines the effectiveness of a professional development program designed specifically to provide foundational knowledge and skills to Technology Teachers in preparation for a transition to a Technology Integration Specialist position. Specifically, it evaluates the Technology Teachers' changes in knowledge and beliefs as a…

Assessing medical technologies in development; a new paradigm of medical technology assessment

NARCIS (Netherlands)

Hummel, J. Marjan; van Rossum, Wouter; Verkerke, Gijsbertus Jacob; Rakhorst, Gerhard

2000-01-01

Objective: Our study aims to provide a practical contribution to the field of medical technology assessment within a new paradigm. This paradigm indicates the need for more comprehensive technology assessments in the development stage of a new technology. - Method: We introduce a method, based on

Assessing medical technologies in development - A new paradigm of medical technology assessment

NARCIS (Netherlands)

Hummel, MJM; van Rossum, W; Verkerke, GJ; Rakhorst, G

2000-01-01

Objective: Our study aims to provide a practical contribution to the field of medical technology assessment within a new paradigm. This paradigm indicates the need for more comprehensive technology assessments in the development stage of a new technology. Method: We introduce a method, based on

77 FR 13159 - Nanoscale Science, Engineering, and Technology Subcommittee of the Committee on Technology...

Science.gov (United States)

2012-03-05

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering, and Technology... public meeting. SUMMARY: The National Nanotechnology Coordination Office (NNCO), on behalf of the Nanoscale Science, Engineering, and Technology (NSET) Subcommittee of the Committee on Technology, National...

Geothermal energy technology

Energy Technology Data Exchange (ETDEWEB)

1977-01-01

Geothermal energy research and development by the Sunshine Project is subdivided into five major categories: exploration and exploitation technology, hot-water power generation technology, volcanic power generation technology, environmental conservation and multi-use technology, and equipment materials research. The programs are being carried out by various National Research Institutes, universities, and private industry. During 1976 and 1977, studies were made of the extent of resources, reservoir structure, ground water movement, and neotectonics at the Onikobe and Hachimantai geothermal fields. Studies to be performed in the near future include the use of new prospecting methods, including artificial magnetotellurics, heat balance calculation, brightspot techniques, and remote sensing, as well as laboratory studies of the physical, mechanical, and chemical properties of rock. Studies are continuing in the areas of ore formation in geothermal environments, hot-dry-rock drilling and fracturing, large scale prospecting technology, high temperature-pressure drilling muds and well cements, and arsenic removal techniques.

Fusion technology programme

International Nuclear Information System (INIS)

Finken, D.

1984-04-01

KfK participates to the Fusion Technology Programme of the European Community. Most of the work in progress addresses the Next European Torus (NET) and the long term technology aspects as defined in the 82/86 programme. A minor part serves to preparation of future contributions and to design studies on fusion concepts in a wider perspective. The Fusion Technology Programme of Euratom covers mainly aspects of nuclear engineering. Plasma engineering, heating, refueling and vacuum technology are at present part of the Physics Programme. In view of NET, integration of the different areas of work will be mandatory. KfK is therefore prepared to address technical aspects beyond the actual scope of the physics experiments. The technology tasks are reported project wise under title and code of the Euratom programme. Most of the projects described here are shared with other European fusion laboratories as indicated in the table annexed to this report. (orig./GG)

Decontamination technology assessment

International Nuclear Information System (INIS)

Allen, R.P.; Konzek, G.J.; Schneider, K.R.; Smith, R.I.

1988-10-01

This study identifies and technically assesses foreign decontamination and decommissioning (D and D) technology developments that may represent significant improvements over D and D technology currently available or under development in the United States. Technology need areas for nuclear power reactor decommissioning operations were identified and prioritized using the results of past light water reactor (LWR) decommissioning studies to quantitatively evaluate the potential for reducing cost and decommissioning worker radiation dose for each major decommissioning activity. Based on these identified needs, current foreign D and D technologies of potential interest to the US were identified through personal contacts and the collection and review of an extensive body of D and D literature. These technologies were then assessed qualitatively to evaluate their uniqueness, potential for a significant reduction in D and D costs and/or worker radiation dose, development status, and other factors affecting their value and applicability to US needs. 4 refs

Immersive Learning Technologies

Science.gov (United States)

2009-08-20

Immersive Learning Technologies Mr. Peter Smith Lead, ADL Immersive Learning Team 08/20/2009 Report Documentation Page Form ApprovedOMB No. 0704...to 00-00-2009 4. TITLE AND SUBTITLE Immersive Learning Technologies 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR...unclassified c. THIS PAGE unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 Why Immersive Learning Technologies

HETEROGENEOUS INTEGRATION TECHNOLOGY

Science.gov (United States)

2017-08-24

AFRL-RY-WP-TR-2017-0168 HETEROGENEOUS INTEGRATION TECHNOLOGY Dr. Burhan Bayraktaroglu Devices for Sensing Branch Aerospace Components & Subsystems...Final September 1, 2016 – May 1, 2017 4. TITLE AND SUBTITLE HETEROGENEOUS INTEGRATION TECHNOLOGY 5a. CONTRACT NUMBER In-house 5b. GRANT NUMBER N/A...provide a structure for this review. The history and the current status of integration technologies in each category are examined and product examples are

Water Technology Lecture 1: Introducing Water Technology

OpenAIRE

Gray, Nicholas Frederick

2017-01-01

This is a full set of PowerPoint lectures for a course in Water Technology currently given at Trinity College, University of Dublin by professor N.F. Gray. The lectures cover all aspects of water and wastewater treatment and are available for use to lecturers or those interested in the subject. The lecture series is to be used in conjunction with the new textbook ?Water Science and Technology? (4th edition) published by CRC Press in 2017. Lecture 1 is an introduction to the water indust...

The international nuclear technology

International Nuclear Information System (INIS)

Remick, F.J.

1992-01-01

With today's technology, isolationism is virtually impossible. The world's economies are so strongly intertwined that what affects one country will, in some way, influence another. Nuclear technology is no exception. If anything, nuclear technology is a catalyst for international cooperation. In the United States of America, nuclear technology is undergoing significant changes. Many of these changes are being greatly influenced by programs of international cooperation

Annual Science and Engineering Technology Conference/DOD Technology Exposition (7th). Volume 2. Wednesday - Thursday

Science.gov (United States)

2006-04-20

Disruptive Technologies • Army Approach to Disruptive Technologies and Transition Mr. Dennis Schmidt, Director, Science & Technology Integration, Office of...the Assistant Secretary of the Army for Research and Technology • Navy Approach to Disruptive Technologies and Transition Mr. Lewis DeSandre, Program...Manager, ONR 351 • Air Force Approach to Disruptive Technologies and Transition Colonel Mark Stephen, Associate Deputy Assistant Secretary (Science

Technology Catalogue. First edition

Energy Technology Data Exchange (ETDEWEB)

1994-02-01

The Department of Energy`s Office of Environmental Restoration and Waste Management (EM) is responsible for remediating its contaminated sites and managing its waste inventory in a safe and efficient manner. EM`s Office of Technology Development (OTD) supports applied research and demonstration efforts to develop and transfer innovative, cost-effective technologies to its site clean-up and waste management programs within EM`s Office of Environmental Restoration and Office of Waste Management. The purpose of the Technology Catalogue is to provide performance data on OTD-developed technologies to scientists and engineers assessing and recommending technical solutions within the Department`s clean-up and waste management programs, as well as to industry, other federal and state agencies, and the academic community. OTD`s applied research and demonstration activities are conducted in programs referred to as Integrated Demonstrations (IDs) and Integrated Programs (IPs). The IDs test and evaluate.systems, consisting of coupled technologies, at specific sites to address generic problems, such as the sensing, treatment, and disposal of buried waste containers. The IPs support applied research activities in specific applications areas, such as in situ remediation, efficient separations processes, and site characterization. The Technology Catalogue is a means for communicating the status. of the development of these innovative technologies. The FY93 Technology Catalogue features technologies successfully demonstrated in the field through IDs and sufficiently mature to be used in the near-term. Technologies from the following IDs are featured in the FY93 Technology Catalogue: Buried Waste ID (Idaho National Engineering Laboratory, Idaho); Mixed Waste Landfill ID (Sandia National Laboratories, New Mexico); Underground Storage Tank ID (Hanford, Washington); Volatile organic compound (VOC) Arid ID (Richland, Washington); and VOC Non-Arid ID (Savannah River Site, South Carolina).

CONDITIONS AND ORGANIZATION OF THE TRANSITION TO BASIC TECHNOLOGIES OF A NEW TECHNOLOGICAL STRUCTURE

Directory of Open Access Journals (Sweden)

B. L. Bourov

2011-01-01

Full Text Available With due account for the coming new (VI-th world technological structure, future creation of new types of industrial production is both possible and necessary. Economic environment conditions favorable for such development are designated. In reference to Russian technological environment particulars, self-developing economic-technological microenvironment of a new quality level should be created in zones where controlled «technological chains» function. Possibilities of creation of the VI-th technological structure level basic technologies are shown for industrial and household waste processing techniques as an example.

MINERGY CORPORATION GLASS FURNACE TECHNOLOGY EVALUATION: INNOVATION TECHNOLOGY EVALUATION REPORT

Science.gov (United States)

This report presents performance and economic data for a U.S. Environmental Protection Agency (EPA) Superfund Innovative Technology Evaluation (SITE) Program demonstration of the Minergy Corporation (Minergy) Glass Furnace Technology (GFT). The demonstration evaluated the techno...

Science and Technology Teachers' Views of Primary School Science and Technology Curriculum

Science.gov (United States)

Yildiz-Duban, Nil

2013-01-01

This phenomenographic study attempts to explicit science and technology teachers' views of primary school science and technology curriculum. Participants of the study were selected through opportunistic sampling and consisted of 30 science and technology teachers teaching in primary schools in Afyonkarahisar, Turkey. Data were collected through an…

Digital Technology Entrepreneurship

DEFF Research Database (Denmark)

Giones, Ferran; Brem, Alexander

2017-01-01

Technology entrepreneurship is an established concept in academia. However, recent developments in the context of digital entrepreneurship call for revision and advance- ment. The multiple possible combinations of technology and entrepreneurship have res- ulted in a diversity of phenomena...... with significantly different characteristics and socio-economic impact. This article is focused on the identification and description of technology entrepreneurship in times of digitization. Based on current examples, we identify and describe characterizations of technology entrepreneurship, digital techno- logy...... entrepreneurship, and digital entrepreneurship. With this new delineation of terms, we would like to foster discussion between researchers, entrepreneurs, and policy makers on the impact of digitization on entrepreneurship, and set a future research agenda....

Power conversion technologies

Energy Technology Data Exchange (ETDEWEB)

Newton, M. A.

1997-02-01

The Power Conversion Technologies thrust area identifies and sponsors development activities that enhance the capabilities of engineering at Lawrence Livermore National Laboratory (LLNL) in the area of solid- state power electronics. Our primary objective is to be a resource to existing and emerging LLNL programs that require advanced solid-state power electronic technologies.. Our focus is on developing and integrating technologies that will significantly impact the capability, size, cost, and reliability of future power electronic systems. During FY-96, we concentrated our research efforts on the areas of (1) Micropower Impulse Radar (MIR); (2) novel solid-state opening switches; (3) advanced modulator technology for accelerators; (4) compact accelerators; and (5) compact pulse generators.

Superconducting technology

International Nuclear Information System (INIS)

2010-01-01

Superconductivity has a long history of about 100 years. Over the past 50 years, progress in superconducting materials has been mainly in metallic superconductors, such as Nb, Nb-Ti and Nb 3 Sn, resulting in the creation of various application fields based on the superconducting technologies. High-T c superconductors, the first of which was discovered in 1986, have been changing the future vision of superconducting technology through the development of new application fields such as power cables. On basis of these trends, future prospects of superconductor technology up to 2040 are discussed. In this article from the viewpoints of material development and the applications of superconducting wires and electronic devices. (author)

Buildings R&D Breakthroughs. Technologies and Products Supported by the Building Technologies Program

Energy Technology Data Exchange (ETDEWEB)

none,

2012-04-01

This report identifies and characterizes commercially available products and emerging (near-commercial) technologies that benefited from the support of the Building Technologies Program (BTP) within the U.S. Department of Energy’s Office of Energy Efficiency and Renewable Energy. The investigation specifically focused on technology-oriented research and development (R&D) projects sponsored by BTP’s Emerging Technologies subprogram from 2005-2009.

Use of Payment Technology

DEFF Research Database (Denmark)

Xiao, Xiao; Hedman, Jonas; Runnemark, Emma

2015-01-01

Drawing on the theory of consumption value, this research-in-progress strives to provide a theoretical explanation of payment technology use by investigating the relationship between consumers’ perceptions of different consumption values associated with a certain payment technology and their choice...... to use the technology. We conducted the study in the context of Denmark, a Northern European country, with three well established payment technologies: cash, payment cards, and Internet banking. Following a focus group of identifying and defining four types of consumption values associated with each...... payment technology, a survey was then conducted by a national statistics agency in the country. Preliminary results have shown that different consumption values matter for the use of different payment technologies. The findings will potentially contribute to a better understanding of consumer payment...

Technology monitoring; Technologie-Monitoring

Energy Technology Data Exchange (ETDEWEB)

Eicher, H.; Rigassi, R. [Eicher und Pauli AG, Liestal (Switzerland); Ott, W. [Econcept AG, Zuerich (Switzerland)

2003-07-01

This study made for the Swiss Federal Office of Energy (SFOE) examines ways of systematically monitoring energy technology development and the cost of such technologies in order to pave the way to a basis for judging the economic development of new energy technologies. Initial results of a survey of the past development of these technologies are presented and estimates are made of future developments in the areas of motor-based combined heat and power systems, fuel-cell heating units for single-family homes and apartment buildings, air/water heat pumps for new housing projects and high-performance thermal insulation. The methodology used for the monitoring and analysis of the various technologies is described. Tables and diagrams illustrate the present situation and development potential of various fields of technology.

Nuclear technology and beyond

International Nuclear Information System (INIS)

Akiyama, Mamoru

1997-01-01

After the confrontation of East and West, and the problem of North and South, we are now facing the era of Globalization in the presence of twenty-first century. Tracing the history of civilization, human being has progressed along with the accumulation of experience, and the development of science and technology. Science and technology bloomed in modern ages, especially, energy technology showed the giant leap in this century. Nuclear science and technology has been developed for peaceful purposes, and for the benefit of humanity. As a result, today, its progress led nuclear science and technology to have the great applicability to the development of the society. Toward the twenty-first century and Globalization, the science and technology developed in nuclear field is hoped to play a great contribution in various area of the society. (author)

Technology Push, Demand Pull And The Shaping Of Technological Paradigms - Patterns In The Development Of Computing Technology

NARCIS (Netherlands)

J.C.M. van den Ende (Jan); W.A. Dolfsma (Wilfred)

2002-01-01

textabstractAn assumption generally subscribed in evolutionary economics is that new technological paradigms arise from advances is science and developments in technological knowledge. Demand only influences the selection among competing paradigms, and the course the paradigm after its inception. In

Disruptive Technology: An Uncertain Future

Science.gov (United States)

2005-05-21

Technology that overturns market -- Military - Technology that causes a fundamental change in force structure, basing, and capability balance * Disruptive Technologies may arise from systems or enabling technology.

Demonstrating and implementing innovative technologies: Case studies from the USDOE Office of Technology Development

International Nuclear Information System (INIS)

Brouns, T.M.; Koegler, K.J.; Mamiya, L.S.

1995-02-01

This paper describes elements of success for demonstration, evaluation, and transfer for deployment of innovative technologies for environmental restoration. They have been compiled from lessons learned through the US Department of Energy (DOE) Office of Technology Development's Volatile Organic Compounds in Arid Soil Integrated Demonstration (VOC-Arid ID). The success of the VOC-Arid ID program was determined by the rapid development demonstration, and transfer for deployment of technologies to operational sites that improve on safety, cost, and/or schedule of performance over baseline technologies. The VOC-Arid ID successfully fielded more than 25 innovative technology field demonstrations; several of the technologies demonstrated have been successfully transferred for deployment Field demonstration is a critical element in the successful transfer of innovative technologies into environmental restoration operations. The measures of success for technology demonstrations include conducting the demonstration in a safe and controlled environment and generating the appropriate information by which to evaluate the technology. However, field demonstrations alone do not guarantee successful transfer for deployment There are many key elements throughout the development and demonstration process that have a significant impact on the success of a technology. This paper presents key elements for a successful technology demonstration and transfer for deployment identified through the experiences of the VOC-Arid ID. Also, several case studies are provided as examples

JPRS Report, Science & Technology, USSR: Science & Technology Policy

Science.gov (United States)

1990-09-18

urgent and vital problems that faced the United States of America at that time. This discussion was of a completely free nature, and, although at...imeni M.O. Auezov, and the Sociology Center attached to the Institute of Eco- nomics, which took part in sociolinguistic studies of the language...development of advanced technologies. In the United States of America , for example, a special fund of these technologies was estab- lished. An

EPA [Environmental Protection Agency] SITE [Superfund Innovative Technology Evaluation] program seeks technology proposals

International Nuclear Information System (INIS)

Anon.

1989-01-01

EPA will issue an RFP to initiate the SITE-005 solicitation for demonstration of technologies under the Superfund Innovative Technology Evaluation (SITE) Program. This portion of the SITE program offers a mechanism for conducting a joint technology demonstration between EPA and the private sector. The goal of the demonstration program is to provide an opportunity for developers to demonstrate the performance of their technologies on actual hazardous wastes at Superfund sites, and to provide accurate and reliable data on that performance. Technologies selected must be of commercial scale and provide solutions to problems encountered at Superfund Sites. Primary emphasis in the RFP is on technologies that address: treatment of mixed, low level radioactive wastes in soils and groundwater; treatment of soils and sludges contaminated with organics and/or inorganics, materials handling as a preliminary step to treatment or further processing, treatment trains designed to handle specific wastes, are in situ technologies, especially those processes providing alternatives to conventional groundwater pump and treat techniques

Technology Foresight on Emerging Technologies: Implications for a National Innovation Initiative in Brazil

Directory of Open Access Journals (Sweden)

Maria Fatima Ludovico de Almeida

2015-07-01

Full Text Available Prospective studies about emerging technologies and their implications for public policy formulation indicate critical choices ranging from global to national level, even to the individual firm or institution. Emerging technologies have been shaping the future of some industries and transforming many others. In many cases, these technologies will determine the restructuring of industries as never before. Specially designed for enabling better planning and future decisions, technology foresight (TF methods are used to foresee diffusion of innovations, mapping out commercially viable roadmaps for technological development. This paper is concerned with a methodological instrument adopted in Brazil as support for building the Agenda for a National Innovation Initiative (NII, which was articulated by government, universities, R&D institutions, and private firms. It presents and discusses an integrated methodological approach for a TF study, specially designed for the purpose of this Brazilian innovation policy instrument, concerning three emerging technologies – nanotechnology, biotechnology, and information and communication technologies (ICT.

Radiation Technology

International Nuclear Information System (INIS)

1990-01-01

The conference was organized to evaluate the application directions of radiation technology in Vietnam and to utilize the Irradiation Centre in Hanoi with the Co-60 source of 110 kCi. The investigation and study of technico-economic feasibility for technology development to various items of food and non-food objects was reported. (N.H.A)

Lasers. Technology Learning Activity. Teacher Edition. Technology Education Series.

Science.gov (United States)

Oklahoma State Dept. of Vocational and Technical Education, Stillwater. Curriculum and Instructional Materials Center.

This document contains the materials required for presenting an 8-day competency-based technology learning activity (TLA) designed to introduce students in grades 6-10 to advances and career opportunities in the field of laser technology. The guide uses a series of hands-on exploratory experiences into which activities to help students develop…

Technology Familiarization to Preservice Teachers: Factors that Influence Teacher Educators' Technology Decisions

Science.gov (United States)

Kalonde, Gilbert; Mousa, Rabab

2016-01-01

The purpose of this study was to investigate factors that influence teacher educators' technology decisions in methods courses. Research has shown various reasons why teachers use different types of technologies and not able to integrate certain technologies. However, this study focused on the source of teachers' instructional technology…

Technology transfer? The rise of China and India in green technology sectors

DEFF Research Database (Denmark)

Lema, Rasmus; Lema, Adrian

2012-01-01

International technology transfer is central to the debate about how to curb the carbon emissions from rapid economic growth in China and India. But given China and India's great progress in building innovation capabilities and green industries, how relevant is technology transfer...... for these countries? This paper seeks insights from three green technology sectors in both countries: wind power, solar energy and electric and hybrid vehicles. We find that, conventional technology transfer mechanisms such as foreign direct investments and licensing, were important for industry formation and take...

What's special about human technology?

OpenAIRE

Robert Aunger

2010-01-01

Human technology is difficult to understand because it is so complex. However, human technology evolved from the simpler technologies of other species. Comparison with these other technologies should illuminate why human technology is distinct. Some birds and primates make tools, or simple technological objects whose function is closely related to their form. Humans, on the other hand, make machines--relatively complex objects whose functionality derives from the interaction of parts with res...

Building Technologies Program Multi-Year Program Plan Technology Validation and Market Introduction 2008

Energy Technology Data Exchange (ETDEWEB)

None, None

2008-01-01

Building Technologies Program Multi-Year Program Plan 2008 for technology validation and market introduction, including ENERGY STAR, building energy codes, technology transfer application centers, commercial lighting initiative, EnergySmart Schools, EnergySmar

ADVANTAGES OF GREEN TECHNOLOGY

OpenAIRE

Ghanshyam Das Soni

2017-01-01

Technology is application of knowledge to practical requirements. Green technologies encompass various aspects of technology which help us reduce the human impact on the environment and create ways of sustainable development. Social equitability, economic feasibility and sustainability are the key parameters for green technologies. Today the environment is racing towards the tipping point at which we would have done permanent irreversible damage to the planet earth. Our current actions are pu...

Digital Sensor Technology

Energy Technology Data Exchange (ETDEWEB)

Ted Quinn; Jerry Mauck; Richard Bockhorst; Ken Thomas

2013-07-01

The nuclear industry has been slow to incorporate digital sensor technology into nuclear plant designs due to concerns with digital qualification issues. However, the benefits of digital sensor technology for nuclear plant instrumentation are substantial in terms of accuracy, reliability, availability, and maintainability. This report demonstrates these benefits in direct comparisons of digital and analog sensor applications. It also addresses the qualification issues that must be addressed in the application of digital sensor technology.

Next wave EM technology : Electromagnetic communication technology continues to progress

International Nuclear Information System (INIS)

Ludwick, J.

1998-01-01

Alpine Oil Services Corp. and Ryan Energy Technologies Inc., have made technological advances in the use of real time electromagnetic (EM) data transmission, using low frequency radio waves to transmit well commands or geological information. The development of the telemetry activated tool was done in two steps. The first technology was real time EM data transmission from the subsurface which used the wellbore to transfer information. The second step was constructing a memory pack which involved an electronic instrument installed in the wellbore which was programmed to perform certain tasks at certain times by transmitting signals back and forth. The use of EM communication allows the geological steering information to come back faster. The EM signal is much faster compared to MWD systems for deeper directional wells. The EM technology also has immediate applications in underbalanced drilling. 1 fig