The preservation of lotus seeds by irradiation technique

International Nuclear Information System (INIS)

Lan Junming; Tang Chuandao

1990-08-01

The preservation test results of lotus seeds by gamma-ray irradiation technique are introduced. The results showed that the sitophilus, penicillus and aspergillus oryzae caused the great damage of lotus seeds. It was found that the radiation doses of deactivation for the insects and the mould were 0.8 kGy and 5 kGy respectively. The irradiated seeds packaged in the cloth bags and stored in the storehouse were not damaged by the insects and moulds for three years. The measured resutls also showed that the nutrient compositions were not changed and the pathogenic bacteria were not found. The irradiated lotus seeds are healthy and safe to the people

Lithium Blanket Module dosimetry measurements at the LOTUS 14-MeV neutron source facility

International Nuclear Information System (INIS)

Tsang, F.Y.; Leo, W.R.; Sahraoui, C.; Wuthrich, S.; Harker, Y.D.

1986-01-01

This paper describes the measurements and results of the dosimeter material reaction rates inside the Lithium Blanket Module (LBM) after irradiation by the LOTUS 14-MeV neutron source at the Ecole Polytechnique Federale de Lausanne. The measurement program has been designed to utilize sets of passive dosimeter materials in the form of foils and wires. The dosimetry materials reaction thresholds and interaction response ranges chosen for this series of measurements encompass the entire neutron spectra along the full length of the LBM fuel rods

The Lotus japonicus genome

DEFF Research Database (Denmark)

Fabaceae, groundbreaking genetic and genomic research has established a significant body of knowledge on Lotus japonicus, which was adopted as a model species more than 20 years ago. The diverse nature of legumes means that such research has a wide potential and agricultural impact, for example...

Analysis of the lbm experiments at lotus

International Nuclear Information System (INIS)

Stepenek, J.; Higgs, C.E.; Pelloni, S.; Davidson, J.W.; Dudziak, D.J.

1986-01-01

The irradiation of the LBM at the LOTUS facility is analysed using a three-dimensional model with the Monte Carlo code MCNP and a two-dimensional r-z model with the deterministic transport code TRISM. A sensitivity and uncertainty analysis based on the 2-D model was performed using the sensitivity code SENSIT-2D. The JEF-1/EFF and ENDF/B-V libraries were used for transport calculations. The COVFILS-2 covariance and uncertainty library based on ENDF/B-V was used for sensitivity and uncertainty analyses. A good agreement between JEF-l/EFF and ENDF/B-V libraries was achieved. The uncertainty in the calculated tritium breeding ratio by the indirect term of the overall cross-section uncertainties varies in the dependence on the position in the LBM from 1.4% (front) to 35.8% (back)

Characteristics of Heavy Metals Contamination in Lotus Root in the Dongting Lake Area, China

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LUO Man

2016-11-01

Full Text Available Heavy metal contamination in soils in the Dongting Lake areas has evoked widespread concerns about the excessive heavy metals in aquatic product. Based on the national standards of food contaminant limits and the method of comprehensive pollution index, heavy metals of Cd, Pb, Cu, Zn, Mn in lotus root were clarified through field investigation in the Dongting Lake area. Results showed that lotus root in the Dongting Lake area was contaminated seriously by heavy metals. Cd and Pb were two main pollutants and the single pollution indices were 5.70 and 8.35 respectively. According to the comprehensive pollution index of heavy metals, lotus root in Yueyanglou District and Yuanjiang City were classified into medium pollution and Junshan District, Huarong County, Nan County, and Datong District were classified into heavy pollution. Principal component analysis showed that planting areas of lotus root were clumped and medium and heavy pollution areas were separated significantly. Habitat contamination by heavy metals and decreasing area of lotus ponds were two main factors for excessive heavy metals in lotus root. Thus, some measurements, such as habit restoration, were proposed for local government to decrease heavy metals in planting areas and to promote the healthy development of lotus root industry in the Dongting Lake area.

Induced Mutation in Yellow Lotus by Gamma Irradiation

International Nuclear Information System (INIS)

Puripunyavanich, Vichai; Boonsirichai, Kanokporn

2006-01-01

Rhizomes of American yellow lotus were irradiated at eht dosed of 0, 10, 20 and 30 Gy, 10 rhizomes per treatment. They were planted in nursery pots. Unirradiated rhizomes gave out new shoots within 3-4 days. The survival rates of the 10, 20 and 30 Gy irradiated rhizomes at one month after transplanted were 80%, 30% and 10%, respectively. The radiation dose that resulted in a 50% death rate (LD 5 0) was approximately 17 Gy. The surviving plant lets were transplanted and grown in pots as the Agricultural Occupation Promotion and Development Center in Chiangmai and Chiangrai for three years. Normally, American yellow lotus does not flower in Thailand. However, a mutant was found to bear flower in Thailand. The mutant flower appeared a little different from the wild-type flower. The tip of its petals was more rounded than the pointy wild-type tip. The mutant will be propagated for clonal production or for use as a par net in breeding crosses with Thai white and pink lotuses

Genome of the long-living sacred lotus (Nelumbo nucifera Gaertn.).

Science.gov (United States)

Ming, Ray; VanBuren, Robert; Liu, Yanling; Yang, Mei; Han, Yuepeng; Li, Lei-Ting; Zhang, Qiong; Kim, Min-Jeong; Schatz, Michael C; Campbell, Michael; Li, Jingping; Bowers, John E; Tang, Haibao; Lyons, Eric; Ferguson, Ann A; Narzisi, Giuseppe; Nelson, David R; Blaby-Haas, Crysten E; Gschwend, Andrea R; Jiao, Yuannian; Der, Joshua P; Zeng, Fanchang; Han, Jennifer; Min, Xiang Jia; Hudson, Karen A; Singh, Ratnesh; Grennan, Aleel K; Karpowicz, Steven J; Watling, Jennifer R; Ito, Kikukatsu; Robinson, Sharon A; Hudson, Matthew E; Yu, Qingyi; Mockler, Todd C; Carroll, Andrew; Zheng, Yun; Sunkar, Ramanjulu; Jia, Ruizong; Chen, Nancy; Arro, Jie; Wai, Ching Man; Wafula, Eric; Spence, Ashley; Han, Yanni; Xu, Liming; Zhang, Jisen; Peery, Rhiannon; Haus, Miranda J; Xiong, Wenwei; Walsh, James A; Wu, Jun; Wang, Ming-Li; Zhu, Yun J; Paull, Robert E; Britt, Anne B; Du, Chunguang; Downie, Stephen R; Schuler, Mary A; Michael, Todd P; Long, Steve P; Ort, Donald R; Schopf, J William; Gang, David R; Jiang, Ning; Yandell, Mark; dePamphilis, Claude W; Merchant, Sabeeha S; Paterson, Andrew H; Buchanan, Bob B; Li, Shaohua; Shen-Miller, Jane

2013-05-10

Sacred lotus is a basal eudicot with agricultural, medicinal, cultural and religious importance. It was domesticated in Asia about 7,000 years ago, and cultivated for its rhizomes and seeds as a food crop. It is particularly noted for its 1,300-year seed longevity and exceptional water repellency, known as the lotus effect. The latter property is due to the nanoscopic closely packed protuberances of its self-cleaning leaf surface, which have been adapted for the manufacture of a self-cleaning industrial paint, Lotusan. The genome of the China Antique variety of the sacred lotus was sequenced with Illumina and 454 technologies, at respective depths of 101× and 5.2×. The final assembly has a contig N50 of 38.8 kbp and a scaffold N50 of 3.4 Mbp, and covers 86.5% of the estimated 929 Mbp total genome size. The genome notably lacks the paleo-triplication observed in other eudicots, but reveals a lineage-specific duplication. The genome has evidence of slow evolution, with a 30% slower nucleotide mutation rate than observed in grape. Comparisons of the available sequenced genomes suggest a minimum gene set for vascular plants of 4,223 genes. Strikingly, the sacred lotus has 16 COG2132 multi-copper oxidase family proteins with root-specific expression; these are involved in root meristem phosphate starvation, reflecting adaptation to limited nutrient availability in an aquatic environment. The slow nucleotide substitution rate makes the sacred lotus a better resource than the current standard, grape, for reconstructing the pan-eudicot genome, and should therefore accelerate comparative analysis between eudicots and monocots.

Swimming Characteristics of Bioinspired Helical Microswimmers Based on Soft Lotus-Root Fibers

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Jia Liu

2017-11-01

Full Text Available Various kinds of helical swimmers inspired by E. coli bacteria have been developed continually in many types of researches, but most of them are proposed by the rigid bodies. For the targeted drug delivery, the rigid body may hurt soft tissues of the working region with organs. Due to this problem, the biomedical applications of helical swimmers may be restricted. However, the helical microswimmers with the soft and deformable body are appropriate and highly adaptive in a confined environment. Thus, this paper presents a lotus-root-based helical microswimmer, which is fabricated by the fibers of lotus-root coated with magnetic nanoparticles to active under the magnetic fields. The helical microstructures are derived from the intrinsic biological structures of the fibers of the lotus-root. This paper aims to study the swimming characteristic of lotus-root-based microswimmers with deformable helical bodies. In the initial step under the uniform magnetic actuation, the helical microswimmers are bent lightly due to the heterogeneous distribution of the internal stress, and then they undergo a swimming motion which is a spindle-like rotation locomotion. Our experiments report that the microswimmers with soft bodies can locomote faster than those with rigid bodies. Moreover, we also find that the curvature of the shape decreases as a function of actuating field frequency which is related to the deformability of lotus-root fibers.

Determination of catechin in lotus rhizomes by high-performance liquid chromatography.

Science.gov (United States)

Yan, Shou-Lei; Wang, Qing-Zhang; Peng, Guang-Hua

2009-08-01

A novel method was developed to analyze lotus rhizome polyphenolic catechin using high-performance liquid chromatography (HPLC). The retain time of catechin was 14.72 min under the optimized condition. Mass spectrometry was further employed to qualify and quantify the purity of the catechin peak. Good linearity (R=0.9997) was obtained within the range of 50-1,000 ng. The coefficient of variance was determined as 5.2%, with a recovery rate of 97%. The detection and quantification limitations of catechin were 23 ng and 50 ng, respectively. The catechin level was 0.0025% in the lotus rhizome, and 0.011% in the knot of the lotus rhizome (Nelumbo nucifera cv. 'damao jie'). The optimized conditions of HPLC for catechin detection in the lotus rhizome matrix were as follows: the SuperlcosIL™ LC-18 analytical column (150 mm×4.6 mm, 5 µm), methanol-water-acetic acid (10:90:1, volume ratio) as the mobile phase, an UV detector at 280 nm, a flow rate of 0.8 ml/min, column temperature at 30°C, and an injection volume of 10 µl.

Texture and wettability of metallic lotus leaves

Science.gov (United States)

Frankiewicz, C.; Attinger, D.

2016-02-01

Superhydrophobic surfaces with the self-cleaning behavior of lotus leaves are sought for drag reduction and phase change heat transfer applications. These superrepellent surfaces have traditionally been fabricated by random or deterministic texturing of a hydrophobic material. Recently, superrepellent surfaces have also been made from hydrophilic materials, by deterministic texturing using photolithography, without low-surface energy coating. Here, we show that hydrophilic materials can also be made superrepellent to water by chemical texturing, a stochastic rather than deterministic process. These metallic surfaces are the first analog of lotus leaves, in terms of wettability, texture and repellency. A mechanistic model is also proposed to describe the influence of multiple tiers of roughness on wettability and repellency. This demonstrated ability to make hydrophilic materials superrepellent without deterministic structuring or additional coatings opens the way to large scale and robust manufacturing of superrepellent surfaces.Superhydrophobic surfaces with the self-cleaning behavior of lotus leaves are sought for drag reduction and phase change heat transfer applications. These superrepellent surfaces have traditionally been fabricated by random or deterministic texturing of a hydrophobic material. Recently, superrepellent surfaces have also been made from hydrophilic materials, by deterministic texturing using photolithography, without low-surface energy coating. Here, we show that hydrophilic materials can also be made superrepellent to water by chemical texturing, a stochastic rather than deterministic process. These metallic surfaces are the first analog of lotus leaves, in terms of wettability, texture and repellency. A mechanistic model is also proposed to describe the influence of multiple tiers of roughness on wettability and repellency. This demonstrated ability to make hydrophilic materials superrepellent without deterministic structuring or additional

The Proteome of Seed Development in the Model Legume Lotus japonicus

DEFF Research Database (Denmark)

Dam, Svend; Laursen, Brian S.; Ornfelt, Jane H.

2009-01-01

three developmental phases of legume seeds and the presence of embryo, endosperm, and seed coat in desiccated seeds. Furthermore, protein, oil, starch, phytic acid, and ash contents were determined, and this indicates that the composition of mature Lotus seed is more similar to soybean than to pea......We have characterized the development of seeds in the model legume Lotus japonicus. Like soybean (Glycine max) and pea (Pisum sativum), Lotus develops straight seed pods and each pod contains approximately 20 seeds that reach maturity within 40 days. Histological sections show the characteristic...... proteins corresponding to gene accession numbers were identified for the two phases, respectively. All of the proteome data, including the experimental data and mass spectrometry spectra peaks, were collected in a database that is available to the scientific community via a Web interface (http...

Background and History of the Lotus japonicus Model Legume System

DEFF Research Database (Denmark)

Stougaard, Jens

2014-01-01

The combination of favourable biological features, stable transformation procedures, application of genetics and genome-based global approaches has established Lotus japonicus as a model legume and provided a platform for addressing important biological questions often, but not exclusively......, focusing on endosymbiosis. Several important discoveries have been made, and the Lotus community has contributed novel results, promoting our understanding of plant biology as well as our understanding of properties and characteristics typical for plants belonging to the legume family. Progress has been...

Lotus Dust Mitigation Coating and Molecular Adsorber Coating

Science.gov (United States)

O'Connor, Kenneth M.; Abraham, Nithin S.

2015-01-01

NASA Goddard Space Flight Center has developed two unique coating formulations that will keep surfaces clean and sanitary and contain contaminants.The Lotus Dust Mitigation Coating, modeled after the self-cleaning, water-repellant lotus leaf, disallows buildup of dust, dirt, water, and more on surfaces. This coating, has been successfully tested on painted, aluminum, glass, silica, and some composite surfaces, could aid in keeping medical assets clean.The Molecular Adsorber Coating is a zeolite-based, sprayable molecular adsorber coating, designed to prevent outgassing in materials in vacuums. The coating works well to adsorb volatiles and contaminates in manufacturing and processing, such as in pharmaceutical production. The addition of a biocide would also aid in controlling bacteria levels.

Lotus alianus, a new species from Cabo Verde and nomeenclatural notes on Lotus section Pedrosia (Fabaceae)

Science.gov (United States)

Lotus alianus J.H. Kirkbr., sp. nov., is described and illustrated. It is a rare endemic species from the Republic of Cape Verde, and is found in dry habitats on just two islands, Ilhas de Santo Antao and Sao Vicente. In addition, two species names are synonymized with L. creticus L., and a lectotyp...

Early Lotus japonicus root transcriptomic responses to symbiotic and pathogenic fungal exudates

Directory of Open Access Journals (Sweden)

Marco eGiovannetti

2015-06-01

Full Text Available The objective of this study is to evaluate Lotus japonicus transcriptomic responses to arbuscular mycorrhizal (AM germinated spore exudates (GSE, responsible for activating nuclear Ca2+ spiking in plant root epidermis. A microarray experiment was performed comparing gene expression in Lotus rootlets treated with GSE or water after 24 h and 48 h. The transcriptional pattern of selected genes that resulted to be regulated in the array was further evaluated upon different treatments and timings. In particular, Lotus rootlets were treated with: GSE from the pathogenic fungus Colletotrichum trifolii; short chitin oligomers (acknowledged AM fungal signals and long chitin oligomers (as activators of pathogenic responses. This experimental set up has revealed that AM GSE generates a strong transcriptomic response in Lotus roots with an extensive defense-related response after 24 hours and a subsequent downregulation after 48 hours. A similar subset of defense-related genes resulted to be upregulated also upon treatment with C. trifolii GSE, although with an opposite trend. Surprisingly, long chitin oligomers activated both defense-like and symbiosis-related genes. Among the genes regulated in the microarray, promoter-GUS assay showed that LjMATE1 activates in epidermal cells and root hairs.

Effects of micro- and nano-structures on the self-cleaning behaviour of lotus leaves

Science.gov (United States)

Cheng, Y. T.; Rodak, D. E.; Wong, C. A.; Hayden, C. A.

2006-03-01

When rain falls on lotus leaves water beads up with a high contact angle. The water drops promptly roll off the leaves, collecting dirt along the way. This self-cleaning ability or lotus effect has, in recent years, stimulated much research effort worldwide for a variety of applications ranging from self-cleaning window glasses, paints, and fabrics to low friction surfaces. What are the mechanisms giving rise to the lotus effect? Although chemical composition and surface structure are believed important, a systematic experimental investigation of their effects is still lacking. By altering the surface structure of the leaves while keeping their chemical composition approximately the same, we report in this study the influence of micro- and nano-scale structures on the wetting behaviour of lotus leaves. The findings of this work may help design self-cleaning surfaces and improve our understanding of wetting mechanisms.

Lotus (Nelumbo nucifera) Rhizome as an Antioxidant Dietary Fiber in Cooked Sausage: Effects on Physicochemical and Sensory Characteristics.

Science.gov (United States)

Ham, Youn-Kyung; Hwang, Ko-Eun; Song, Dong-Heon; Kim, Yong-Jae; Shin, Dong-Jin; Kim, Kyung-Il; Lee, Hye-Jin; Kim, Na-Rae; Kim, Cheon-Jei

2017-01-01

The objective of this study was to determine the physicochemical and sensory properties of cooked emulsion sausages containing different levels of lotus rhizome powder (0, 1, 2, and 3%, based on total weight). Lotus rhizome powder had no significant ( p >0.05) impact on pH, moisture, protein, or ash content of sausage. However, fat content was slightly but significantly ( p color of cooked sausage compared to control. Increase in lotus rhizome level slightly improved the emulsion stability and apparent viscosity. Significant ( p color and juiciness scores. However, cooked sausages exhibited similar overall acceptability regardless of the level of lotus rhizome powder added to sausages. Therefore, lotus rhizome powder, an antioxidant dietary fiber, could be used as an effective natural ingredient in meat products for the development of healthier and functional food.

Green synthesis of nitrogen-doped carbon dots from lotus root for Hg(II) ions detection and cell imaging

Energy Technology Data Exchange (ETDEWEB)

Gu, Dan; Shang, Shaoming, E-mail: smshang@jiangnan.edu.cn; Yu, Qin; Shen, Jie

2016-12-30

Graphical abstract: Fluorescent nitrogen-doped carbon dots were synthesized by a facile, green, and fast microwave method, using lotus root as carbon source. These nitrogen-doped carbon dots can be used for Hg{sup 2+} detection and cell imaging. - Highlights: • A green, fast strategy for synthesizing carbon dots was established. • A simple, sensitive, selective and wide linear range sensing of Hg{sup 2+} was developed. • The sensor system was demonstrated to detect Hg{sup 2+} in environmental water sample. • The carbon dots could serve for multicolor fluorescence bioimaging. - Abstract: Herein, a facile, green, and fast method was developed in the synthesis of fluorescent nitrogen-doped carbon dots (CDs) with nitrogen content of 5.23%, using one-pot microwave treatment of lotus root (LR), without using any other surface passivation agents. The results show that these LR-CDs (with an average diameter of 9.41 nm) possess many outstanding features and have a high quantum yield of 19.0%. We further demonstrated applications of LR-CDs as probes for heavy metal ion detection. The LR-CDs exhibit captivating sensitivity and selectivity toward Hg{sup 2+} with a linear range from 0.1 to 60.0 μM and a detection limit of 18.7 nM. Eventually, the LR-CDs were applied for multicolor cell imaging, demonstrating their potential toward diverse applications.

Microscopic observations of condensation of water on lotus leaves

Science.gov (United States)

Cheng, Yang-Tse; Rodak, Daniel E.; Angelopoulos, Anastasios; Gacek, Ted

2005-11-01

We report an in situ observation of water condensation and evaporation on lotus leaf surfaces inside an environmental scanning electron microscope. The real-time observation shows, at the micrometer length scale, how water drops grow to large contact angles during water condensation, and decrease in size and contact angle during the evaporation phase of the experiment. To rationalize the observations, we propose a geometric model for liquid drops on rough surfaces when the size of the drop and surface roughness scale are comparable. This model suggests that when drop size and surface roughness are of the same magnitude, such as micrometer size water drops on lotus leaves, well-known equations for wetting on rough surfaces may not be applicable.

The Lotus japonicus ndx gene family is involved in nodule function and maintenance

DEFF Research Database (Denmark)

Grønlund, Mette; Gustafsen, Camilla; Jensen, Dorthe Bødker

2003-01-01

To elucidate the function of the ndx homeobox genes during the Rhizobium-legume symbiosis, two Lotus japonicus ndr genes were expressed in the antisense orientation under the control of the nodule-expressed promoter Psenod12 in transgenic Lotus japonicus plants. Many of the transformants obtained...

Tritium assay of Li2O pellets in the LBM/LOTUS experiments

International Nuclear Information System (INIS)

Quanci, J.; Azam, S.; Bertone, P.

1986-01-01

One of the objectives of the Lithium Blanket Module (LBM) program is to test the ability of advanced neutronics codes to model the tritium breeding characteristics of a fusion blanket exposed to a toroidal fusion neutron source. The LBM consists of over 20,000 cylindrical lithium oxide pellets and numerous diagnostic pellets and wafers. The LBM has been irradiated at the Ecole Polytechnique Federale de Lausanne (EPFL) LOTUS facility with a Haefely sealed neutron generator that gives a point deuterium-tritium neutron source up to 5 x 10 12 14-MeV n/s. Both Princeton Plasma Physics Lab. (PPPL) and EPFL assayed the tritium bred at various positions in the LBM. EPFL employed a dissolution technique while PPPL recovered the tritium by a thermal extraction method

Lotus-leaf-like structured chitosan–polyvinyl pyrrolidone films as an anti-adhesion barrier

Energy Technology Data Exchange (ETDEWEB)

Lim, Jin Ik; Kang, Min Ji; Lee, Woo-Kul, E-mail: leewo@dankook.ac.kr

2014-11-30

Highlights: • Improved mechanical properties by hydrogen bond between chitosan and PVP chains. • Improved anti-adhesion effect by lotus-leaf-like structured chitosan–PVP (L-chitosan–PVP) film. • L-Chitosan–PVP film as a blood/tissue anti-adhesion barrier for post-surgical treatment. - Abstract: For postsurgical anti-adhesion barrier applications, lotus-leaf-like structured chitosan–PVP films were prepared using a solution casting method with dodecyltrichloro-immobilized SiO{sub 2} nanoparticles. We evaluated whether the lotus-leaf-like structured chitosan–PVP films (L-chitosan–PVP) could be applied as postsurgical anti-adhesion barriers. A recovery test using a tensile strength testing machine and measurement of crystallinity using X-ray diffraction indicated that films with 75% PVP were the optimal composition of the chitosan–PVP films. Also, dodecyltrichloro-immobilized SiO{sub 2} nanoparticles were synthesized and sprayed on the film after pretreatment with the instant bio-glue. Analysis of cell adhesion, proliferation, and anti-thrombus efficiency were performed via a WST assay, field emission scanning electron microscopy, and hemacytometry. The contact angle with the lotus-leaf-like surface was of approximately 150°. Furthermore, the L-chitosan–PVP film yielded a lower cell and platelet adhesion rate (around less than 4%) than that yielded by the untreated film. These results indicate that the lotus-leaf-like structure has a unique property and that this novel L-chitosan–PVP film can be applied as a blood/tissue-compatible, biodegradable material for implantable medical devices that need an anti-adhesion barrier.

Lotus-leaf-like structured chitosan–polyvinyl pyrrolidone films as an anti-adhesion barrier

International Nuclear Information System (INIS)

Lim, Jin Ik; Kang, Min Ji; Lee, Woo-Kul

2014-01-01

Highlights: • Improved mechanical properties by hydrogen bond between chitosan and PVP chains. • Improved anti-adhesion effect by lotus-leaf-like structured chitosan–PVP (L-chitosan–PVP) film. • L-Chitosan–PVP film as a blood/tissue anti-adhesion barrier for post-surgical treatment. - Abstract: For postsurgical anti-adhesion barrier applications, lotus-leaf-like structured chitosan–PVP films were prepared using a solution casting method with dodecyltrichloro-immobilized SiO 2 nanoparticles. We evaluated whether the lotus-leaf-like structured chitosan–PVP films (L-chitosan–PVP) could be applied as postsurgical anti-adhesion barriers. A recovery test using a tensile strength testing machine and measurement of crystallinity using X-ray diffraction indicated that films with 75% PVP were the optimal composition of the chitosan–PVP films. Also, dodecyltrichloro-immobilized SiO 2 nanoparticles were synthesized and sprayed on the film after pretreatment with the instant bio-glue. Analysis of cell adhesion, proliferation, and anti-thrombus efficiency were performed via a WST assay, field emission scanning electron microscopy, and hemacytometry. The contact angle with the lotus-leaf-like surface was of approximately 150°. Furthermore, the L-chitosan–PVP film yielded a lower cell and platelet adhesion rate (around less than 4%) than that yielded by the untreated film. These results indicate that the lotus-leaf-like structure has a unique property and that this novel L-chitosan–PVP film can be applied as a blood/tissue-compatible, biodegradable material for implantable medical devices that need an anti-adhesion barrier

Sound Absorption and Friction Properties of Nano-Lotus Leaf Coated Concrete for Rigid Pavement

Directory of Open Access Journals (Sweden)

Marcelo GONZALEZ

2016-09-01

Full Text Available This paper presents the feasibility of superhydrophobic films to create the nano-lotus leaf effect on concrete surface and their influence on sound absorption and friction properties of concrete for application in rigid pavements. The study involved an evaluation of nanomaterials at the laboratory scale to analyze the effects of microtexture modification on the friction and sound absorption of concrete pavement. A number of laboratory specimens were produced by applying different amounts of nano-lotus leaf coating on the top of the textured concrete surface. The British pendulum test was used to measure the friction number, and an impedance tube was used to determine the sound absorption coefficient. Laboratory results indicate that nano-lotus leaf coated concrete can maintain the required friction property for rigid pavement, but may not increase the noise absorption. Further research must be carried out to determine possible benefit of the lotus leaf effect for reducing hydroplaning, particularly during heavy rainfall.DOI: http://dx.doi.org/10.5755/j01.ms.22.3.7638

Condensed Tannin Concentrations of Three Lotus Species Grown in Different Environments Contenido de Taninos Condensados en Germoplasma de Tres Especies del Género Lotus en Diferentes Ambientes

Directory of Open Access Journals (Sweden)

Hernán Acuña

2008-03-01

Full Text Available Condensed tannins (CT present in Lotus spp., depending on their concentration in the plant, can prevent bloating and improve protein absorption in ruminants. With the objective to know the variability of this characteristic, the CT concentration (% dry matter basis was determined by the butanol-HCl procedure in 13 cultivars of Lotus corniculatus L. (Lc introduced from North and South America and Oceania, 11 accessions of Lotus tenuis Waldst. & Kit. ex Willd. (Lt, and 22 accessions of Lotus uliginosus Schkuhr (Lu collected in Chile, from 32° to 38° S and 36° to 46° S, respectively. The field experiments, with two randomized complete blocks, were established in autumn 1998, in five different environments in the central-southern zone of Chile (clay soil, sandy soil, and three volcanic soils with different levels of water availability. The herbage sampling was conducted in spring-summer 1999-2000, when the plants were in full flowering. Significant differences between cultivars or accessions were found within each site, in total CT and/or in extractable or protein or fiber bound fractions of CT concentrations. The Lc and Lu genotypes showed high variability, with a range of 4.7 to 8.7% and 6.3 to 11.0%, and means of 6.1 and 8.2%, respectively, of total CT. The Lt general mean was 4.8%, considerably higher than the figures reported in the literature, and had low variability. This information will contribute to select genotypes for the improvement of this species by selection and breedingLos taninos condensados (TC presentes en Lotus spp ., dependiendo de su concentración en la planta, pueden evitar el meteorismo y favorecer la absorción de proteína en rumiantes. Con el objetivo de conocer la variabilidad de esta característica se determinó por el método del butanol-HCl la concentración (% base materia seca de TC en 13 cultivares de Lotus corniculatus L. (Lc introducidos de Norteamérica, Sudamérica y Oceanía, 11 accesiones de Lotus

Activation of an endogenous retrotransposon associated with epigenetic changes in Lotus japonicus

DEFF Research Database (Denmark)

Fukai, Eigo; Stougaard, Jens; Hayashi, Makoto

2013-01-01

Long terminal repeat retrotransposons occupy a large portion of genomes in flowering plants. In spite of their abundance, the majority are silenced and rarely transpose. One of the examples of a highly active retrotransposon is Lotus Retrotransposon 1(LORE1), of the model legume Lotus japonicus...... significance of LORE1 as a member of chromovirus, a chromodomain containing clade of the Gypsy superfamily. Then we discuss possibilities and methodologies for using endogenous transposable elements as mutagens to generate gene tagging populations in plants...

Neutronic analysis for the LBM/LOTUS experiment

International Nuclear Information System (INIS)

Kolibal, J.; Ku, L.P.; Liew, S.L.

1986-01-01

The LBM/LOTUS experimental results will be compared with the calculated values using the MCC in early 1986 based on the MCNP and DOT models described. The comparison will allow a testing of the features of the MCC in a simplified situation, in preparation for the ultimate experiment of the LBM on TFTR

The Sacred Lotus - An Incredible Wealth of Wetlands

Indian Academy of Sciences (India)

Home; Journals; Resonance – Journal of Science Education; Volume 18; Issue 8. The Sacred Lotus - An Incredible Wealth of Wetlands. R N Mandal R Bar. General Article Volume 18 Issue 8 August 2013 pp 732-737. Fulltext. Click here to view fulltext PDF. Permanent link:

Durable Lotus-effect surfaces with hierarchical structure using micro- and nanosized hydrophobic silica particles.

Science.gov (United States)

Ebert, Daniel; Bhushan, Bharat

2012-02-15

Surfaces with a very high apparent water contact angle (CA) and low water contact angle hysteresis (CAH) exhibit many useful characteristics, among them extreme water repellency, low drag for fluid flow, and a self-cleaning effect. The leaf of the Lotus plant (Nelumbo nucifera) achieves these properties using a hierarchical structure with roughness on both the micro- and nanoscale. It is of great interest to create durable surfaces with the so-called "Lotus effect" for many important applications. In this study, hierarchically structured surfaces with Lotus-effect properties were fabricated using micro- and nanosized hydrophobic silica particles and a simple spray method. In addition, hierarchically structured surfaces were prepared by spraying a nanoparticulate coating over a micropatterned surface. To examine the similarities between surfaces using microparticles versus a uniform micropattern as the microstructure, CA and CAH were compared across a range of pitch values for the two types of microstructures. Wear experiments were performed using an atomic force microscope (AFM), a ball-on-flat tribometer, and a water jet apparatus to verify multiscale wear resistance. These surfaces have potential uses in engineering applications requiring Lotus-effect properties and high durability. Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical changes associated with lotus and water lily natto production

Science.gov (United States)

Lestari, S. D.; Fatimah, N.; Nopianti, R.

2017-04-01

Natto is a traditional Japanese food made by fermenting whole soybean seeds with pure culture of Bacillus subtilis subsp. natto. The purpose of this study was to investigate the suitability of lotus (Nelumbo nucifera) and water lily (Nymphaea stellata) seeds as the raw materials for natto production. Chemical (proximate, amino acids and minerals) changes were observed on raw, steamed and fermented seeds. Proximate compositions of all samples were calculated in both wet basis and dry basis. In wet basis calculation, steaming and fermentation tended to lower the carbohydrates, ashes, fats and protein content which were attributed to the increase of moisture. The total amino acid, iron and magnesium contents of raw lotus seeds were 24.29%, 5.08 mg 100g-1 and 174.23 mg 100g-1 dry matter, respectively. After a 24h-fermentation at 40°C, the total amino acids decreased while iron and magnesium contents increased significantly reaching, in respective order, 9.9 mg 100g-1 and 411.36 mg 100g-1 dry matter. Changes in chemical composition after fermentation were more pronounced in lotus seeds than water lily seeds indicating that their nutrient composition were more suitable to support Bacillus subtilis growth.

Morphometric characteristics of Lotus corniculatus L. genotypes ...

African Journals Online (AJOL)

The aim of this study was to examine the degree of variability in morphological and agronomic characteristics of 20 Lotus corniculatus L. local genotypes, and also to set aside germplasm that will be used as a source of genetic basis for improvement of the studied properties. In poor quality soils, L. corniculatus L. plays an ...

Legume and Lotus japonicus Databases

DEFF Research Database (Denmark)

Hirakawa, Hideki; Mun, Terry; Sato, Shusei

2014-01-01

Since the genome sequence of Lotus japonicus, a model plant of family Fabaceae, was determined in 2008 (Sato et al. 2008), the genomes of other members of the Fabaceae family, soybean (Glycine max) (Schmutz et al. 2010) and Medicago truncatula (Young et al. 2011), have been sequenced. In this sec....... In this section, we introduce representative, publicly accessible online resources related to plant materials, integrated databases containing legume genome information, and databases for genome sequence and derived marker information of legume species including L. japonicus...

Separation of Oligosaccharides from Lotus Seeds via Medium-pressure Liquid Chromatography Coupled with ELSD and DAD

Science.gov (United States)

Lu, Xu; Zheng, Zhichang; Miao, Song; Li, Huang; Guo, Zebin; Zhang, Yi; Zheng, Yafeng; Zheng, Baodong; Xiao, Jianbo

2017-03-01

Lotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coupled with evaporative light-scattering detector and diode-array detector, five oligosaccharides were isolated and their structures were further characterized by electrospray ionization-mass spectrometry and gas chromatography-mass spectrometry. In vitro testing determined that LOS3-1 and LOS4 elicited relatively good proliferative effects on Lactobacillus delbrueckii subsp. bulgaricus. These results indicated a structure-function relationship between the physiological activity of oligosaccharides in lotus seeds and the number of probiotics applied, thus providing room for improvement of this particular feature. Intestinal probiotics may potentially become a new effective drug target for the regulation of immunity.

The evolution of plant chemical defence - new roles for hydroxynitrile glucosides in Lotus japonicus

DEFF Research Database (Denmark)

Knudsen, Camilla

Plants are sessile organisms well-known to produce a vast array of chemical compounds of which many are used in chemical defence against herbivores and pathogens. The biosynthesis of these plant chemical defence compounds poses a considerable risk of self-toxicity for the plant itself. Several...... on hydroxynitrile glucoside metabolism in the legume model plant Lotus japonicus. Lotus japonicus produces both cyanogenic and non-cyanogenic hydroxynitrile glucosides as chemical defence compounds. The cyanogenic glucosides linamarin and lotaustralin are stored in the cell vacuole as inactive glycosides and, upon...... function and evolution. Further, it contributes to our understanding of the formation and role of biosynthetic gene clusters in plant chemical defence. The bifurcation in hydroxynitrile glucoside biosynthesis and catabolism observed in Lotus japonicus makes it a very suitable model system to study...

Expanding the Lotus japonicus reverse genetics toolbox – Development of LORE1 retrotransposon mutagenesis and artificial miRNA-mediated silencing

DEFF Research Database (Denmark)

Urbanski, Dorian Fabian

2011-01-01

. The protocols developed in the current project are now the cornerstone of a new LORE1 reverse genetics resource characterized by efficient mutant line generation and accurate mutation annotation. In parallel, artificial microRNAs (amiRNAs) were designed based on both Arabidopsis and Lotus backbones......Currently, the most common approach to studying Lotus japonicus (Lotus) genes is forward genetics in which a gene responsible for the studied phenotype is identified through map-based cloning. In reverse genetics, the activity of a gene of interest is modified to discover its mutant phenotype....... Prior to this project, the only reverse genetics resource available in Lotus was the TILLING resource. In an attempt to advance Lotus genetic studies, present study is focused on the development of two additional resources. The first is based on insertional mutagenesis and the second on harnessing post...

The plant growth promoting substance, lumichrome, mimics starch and ethylene-associated symbiotic responses in lotus and tomato roots

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Liezel eGouws

2012-06-01

Full Text Available Symbiosis involves responses that maintain the plant host and symbiotic partner’s genetic program; yet these cues are far from elucidated. Here we describe the effects of lumichrome, a flavin identified from Rhizobium spp., applied to lotus (Lotus japonicus and tomato (Solanum lycopersicum. Combined transcriptional and metabolite analyses suggest that both species shared common pathways that were altered in response to this application under replete, sterile conditions. These included genes involved in symbiosis, as well as transcriptional and metabolic responses related to enhanced starch accumulation and altered ethylene metabolism. Lumichrome priming also resulted in altered colonization with either Mesorhizobium loti (for lotus or Glomus intraradices/Glomus mossea (for tomato. It enhanced nodule number but not nodule formation in lotus; while leading to enhanced hyphae initiation and delayed arbuscule maturation in tomato.

STRATEGI PEMASARAN PAKET INBOUND TOUR: STUDI KASUS DI PT. LOTUS ASIA TOURS JIMBARAN BALI

Directory of Open Access Journals (Sweden)

Camelia Agatha Mahayu Putri

2015-12-01

Full Text Available Tujuan penelitian ini adalah untuk mengetahui faktor internal yaitu kekuatan dan kelemahan dan eksternal yaitu peluang dan ancaman dari PT. Lotus Asia Tours serta untuk mengetahui strategi yang dapat dilakukan pihak PT. Lotus Asia Tours untuk memasarkan paket inbound tour. Lokasi penelitian dilakukan di PT. Lotus Asia Tours yang terletak di Jl. By Pass Ngurah Rai No. 18, Jimbaran Bali.. Penelitian ini menggunakan metode deskriptif kualitatif. Teknik pengumpulan data dilakukan dengan cara observasi, wawancara, studi kepustakaan, studi dokumentasi dan didukung dengan daftar pertanyaan yang disebarkan kepada 60 informan. Daftar pertanyaan yang disebarkan berpedoman pada indikator marketing mix 7 P (product, price, place, promotion, physical evidence, people, process. Hasil data yang diperoleh lalu dianalisis dengan IFAS (Internal Factor Analysis Summary dan EFAS ( External Factor Analysis Summary, selanjutnya untuk mengetahui posisi perusahaan digunakan matriks internal eksternal dan untuk mengetahui alternatif strategi digunakan matriks SWOT, sehingga dapat menciptakan strategi pemasaran baru serta mengembangkan program-program yang telah ada agar dapat diterapkan oleh PT. Lotus Asia Tours Jimbaran Bali. Berdasarkan hasil penelitian diperoleh total nilai rata-rata lingkungan internal yaitu sebesar 3,01 dan lingkungan eksternal yaitu sebesar 2,96, dapat diketahui bahwa posisi perusahaan berada di sel IV yang berarti stability strategy dimana strategi yang tetapkan tidak merubah arah strategi yang telah ada.

LOTUS: Adaptive text search for big linked data

NARCIS (Netherlands)

Ilievski, F.; Beek, Wouter; van Erp, Marieke; Rietveld, Laurens; Schlobach, Stefan

2016-01-01

Finding relevant resources on the Semantic Web today is a dirty job: no centralized query service exists and the support for natural language access is limited. We present LOTUS: Linked Open Text Un- leaShed, a text-based entry point to a massive subset of today’s Linked Open Data Cloud. Recognizing

Genome analysis methods: Lotus japonicus [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

Lifescience Database Archive (English)

Full Text Available Lotus japonicus Draft 2n=12 472 Mb 2008 Sanger (Clone-based) ... 315.1 Mb 3-5x Parace...l Genome Assembler 954 110,940 Kazusa Annotation PipelinE for Lotus japonicus (KAPSEL) 37,971 (v2.5) KDRI; http://www.kazusa.or.jp/lotus/ v2.5 v2.5 10.1093/dnares/dsn008 18511435 ...

N-glycan maturation mutants in Lotus japonicus for basic and applied glycoprotein research

DEFF Research Database (Denmark)

Pedersen, Carina T.; Loke, Ian; Lorentzen, Andrea

2017-01-01

Studies of protein N-glycosylation are important for answering fundamental questions on the diverse functions of glycoproteins in plant growth and development. Here we generated and characterised a comprehensive collection of Lotus japonicusLORE1 insertion mutants, each lacking the activity of one...... in the target glyco-genes. For example, both mass spectrometry and immunoblotting experiments suggest that proteins derived from the α1,3-fucosyltransferase (Lj3fuct) mutant completely lacked α1,3-core fucosylation. Mass spectrometry also suggested that the Lotus japonicus convicilin 2 was one of the main...

Preliminary study on irradiation breeding of ornamental lotus

International Nuclear Information System (INIS)

Chen Xiulan; Bao Jianzhong; Liu Chungui; Cao Hong; Zhai Jianqing

2004-01-01

The effects of γ-ray irradiation on seeds and stems of ornamental lotus were studied. The results show that the mutation rate of seeds is higher than that of stems, and 30-60 Gy is the appropriate irradiation dose. The varieties with red or multi-color flower are more mutable than those with white flower. Two varieties were selected

Biogenesis of C-Glycosyl Flavones and Profiling of Flavonoid Glycosides in Lotus (Nelumbo nucifera)

Science.gov (United States)

Li, Shan-Shan; Wu, Jie; Chen, Li-Guang; Du, Hui; Xu, Yan-Jun; Wang, Li-Jing; Zhang, Hui-Jin; Zheng, Xu-Chen; Wang, Liang-Sheng

2014-01-01

Flavonoids in nine tissues of Nelumbo nucifera Gaertner were identified and quantified by high-performance liquid chromatography with diode array detector (HPLC-DAD) and HPLC-electrospray ionization-mass spectrometry (HPLC-ESI-MSn). Thirty-eight flavonoids were identified; eleven C-glycosides and five O-glycosides were discovered for the first time in N. nucifera. Most importantly, the C-glycosyl apigenin or luteolin detected in lotus plumules proved valuable for deep elucidation of flavonoid composition in lotus tissues and for further utilization as functional tea and medicine materials. Lotus leaves possessed the significantly highest amount of flavonoids (2.06E3±0.08 mg 100 g−1 FW) and separating and purifying the bioactive compound, quercetin 3-O-glucuronide, from leaves showed great potential. In contrast, flavonoids in flower stalks, seed coats and kernels were extremely low. Simultaneously, the optimal picking time was confirmed by comparing the compound contents in five developmental phases. Finally, we proposed the putative flavonoid biosynthesis pathway in N. nucifera. PMID:25279809

Combined N-glycome and N-glycoproteome analysis of the Lotus japonicus seed globulin fraction shows conservation of protein structure and glycosylation in legumes

DEFF Research Database (Denmark)

Dam, Svend Secher; Thaysen-Andersen, Morten; Stenkjær, Eva

2013-01-01

Legume food allergy, such as allergy toward peanuts and soybeans, is a health issue predicted to worsen as dietary advice recommends higher intake of legume-based foods. Lotus japonicus (Lotus) is an established legume plant model system for studies of symbiotic and pathogenic microbial...... interactions and, due to its well characterized genotype/phenotype and easily manipulated genome, may also be suitable for studies of legume food allergy. Here we present a comprehensive study of the Lotus N-glycoproteome. The global and site-specific N-glycan structures of Lotus seed globulins were analyzed...

Integrated Emergy and Economic Evaluation of Lotus-Root Production Systems on Reclaimed Wetlands Surrounding the Pearl River Estuary, China

Science.gov (United States)

Lotus (Neumbo nucifera, Gaertn) is the most important aquatic vegetable in China, with a cultivation history of over 3000 years. The emergy, energy, material, and money flows of three lotus root cultivation modes in Wanqingsha, Nansha District, Guangzhou, China were examined usin...

In vivo trypanocidal activity of Nymphaea lotus Linn. methanol extract against Trypanosoma brucei brucei

Directory of Open Access Journals (Sweden)

Muhammad Haruna Garba

2015-10-01

Full Text Available Objective: To evaluate the antitrypanosomal potentials of methanol extract of Nymphaea lotus Linn. (N. lotus with the aim of obtaining a new lead for formulating safe, inexpensive, nontoxic and readily available trypanocidal drugs. Methods: Seventy percent (v/v (methanol/water crude extract of N. lotus was evaluated for antitrypanosomal activity in experimental trypanosomiasis using Trypanosoma brucei bruceiinfected mice. Infected mice in different groups were administered intraperitoneally 100, 200, 300 and 400 mg/kg body weight/day of the crude for two weeks, while a positive control group was treated with standard drug, berenil. Results: The crude extract at a dose of 100 mg/kg body weight/day was more effective than the higher doses in completely clearing parasites from the blood of mice infected with Trypanosoma brucei brucei. Pre-treatment of healthy mice with the crude extract for 5 days before infection did not prevent the establishment of the infection, indicating that the extract had no prophylactic activity. Subinoculation of the blood and cerebrospinal fluid drawn from the cured mice into healthy mice failed to produce any infection within 50 days post inoculation. Administration of 1 000 mg/kg body weight of the crude extract led to the death of 50% of the experimental animals indicating a high level of toxicity of the extract at higher doses. Conclusions: This study has demonstrated the potency of the crude extract of N. lotus in treating experimental trypanosomiasis at lower doses.

Rebrota de Lotus spp. de diferentes hábitos de crescimento Regrowth of Lotus spp. with different growth habits

Directory of Open Access Journals (Sweden)

Rita Poles Maroso

2007-10-01

Full Text Available Este trabalho foi realizado com o objetivo de comparar cultivares de Lotus spp. de diferentes hábitos de crescimento (L. corniculatus: São Gabriel - semi-ereto; ARS2620 - prostrado; L. uliginosus: cv. Maku - decumbente cultivados em vasos e submetidos a diferentes alturas (4 - 8 cm e freqüências (quinzenal - mensal de corte do 95º ao 233º dia de idade. As maiores produções de massa seca (g/planta foram obtidas com cortes mensais a 8 cm (Maku = 13,3; S. Gabriel = 11,5; ARS2620 = 7,3. Em níveis intermediários (mensal - 4 cm; quinzenal - 8 cm, o cultivar Maku foi superior aos demais, enquanto, em cortes quinzenais (4 cm, não houve diferença entre os cultivares. O cultivar ARS2620 apresentou menor estatura e maior cobertura verde residual após os cortes. Na condição residual após o último corte, o cultivar Maku superou os demais quanto ao diâmetro da coroa e às produções de MS da raiz primária, subterrânea, aérea e de sóboles. Todos os cultivares foram favorecidos pelo manejo menos intenso, mas apresentaram mecanismos compensatórios ao serem manejados com cortes baixos em menor freqüência ou com cortes freqüentes, mas menos intensos. Os três cultivares apresentaram caules subterrâneos sobolíferos. A sobrevivência do cultivar São Gabriel e o sistema subterrâneo de Lotus spp. foram comprometidos em cortes intensos e freqüentes.This work aimed to compare Lotus spp. cultivars with different growth habits (L. corniculatus: cv. São Gabriel, semi-erect, cv. ARS2620, prostrate, and L. uliginosus: cv. Maku, decumbent, submitted to different height (4 - 8 cm and cutting frequencies (15 d and 30 d, between the 95th and 233rd days old, grown in pot. Greater DM production (g. plant-1 of DM was obtained under monthly cutting at 8 cm (Maku = 13.3; S. Gabriel = 11.5; ARS2620 = 7.3. Maku surpassed the others when intermediate levels (monthly cutting-4 cm; fortnighly cutting-8 cm were used; harvests every 15 d and at 4 cm resulted

Development of lotus root fermented sugar syrup as a functional food supplement/condiment and evaluation of its physicochemical, nutritional and microbiological properties.

Science.gov (United States)

Shukla, Shruti; Park, Juyeon; Park, Jung Hyun; Lee, Jong Suk; Kim, Myunghee

2018-02-01

Lotus ( Nelumbo nucifera ) root has been used as an edible vegetable in East Asia for thousands of years. The present research was aimed to explore the physicochemical, nutritional and microbiological safety of lotus root fermented sugar syrup as a fermented food supplement or condiment for human health benefits. In this study, the physicochemical, nutritional and microbiological safety properties of lotus root syrup fermented with 57° Brix brown sugar at different time periods until 6 months (180 days) was investigated. There was a significant improvement as compared to 57° Brix brown sugar broth (as a control) in the total acceptability and physicochemical properties of lotus root sugar syrup samples such as pH and color improvement. The red color values of 180 days lotus root fermented sugar syrup samples were significantly enhanced (6.85 ± 0.58) when compared with the control (0.20 ± 0.15). In addition, the total protein content was increased from 8.27 ± 0.86 to 392.33 ± 7.19 μg/mL, along with the increase in fermentation time reaching to the level of consumption acceptability. All the lotus root fermented sugar syrup samples were subjected to microbiological analysis. It was found that the coliform, Bacillus cereus , Escherichia coli , Salmonella and Staphylococcus aureus counts were not detected in majority of the samples, confirming the high degree of hygiene processing of lotus root fermented sugar syrup samples for its use as a food supplement or condiment.

Integrated multi-omics analysis supports role of lysophosphatidylcholine and related glycerophospholipids in the Lotus japonicus-Glomus intraradices mycorrhizal symbiosis.

Science.gov (United States)

Vijayakumar, Vinod; Liebisch, Gerhard; Buer, Benjamin; Xue, Li; Gerlach, Nina; Blau, Samira; Schmitz, Jessica; Bucher, Marcel

2016-02-01

Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus, we performed an integrated multi-omics analysis with a focus on plant and fungal phospholipid (PL) metabolism and biological significance of lysophosphatidylcholine (LPC). Our results support the role of LPC as a bioactive compound eliciting cellular and molecular response mechanisms in Lotus. Evidence is provided for large interspecific chemical diversity of LPC species among mycorrhizae with related AMF species. Lipid, gene expression and elemental profiling emphasize the Lotus-Glomus intraradices interaction as distinct from other arbuscular mycorrhizal (AM) interactions. In G. intraradices, genes involved in fatty acid (FA) elongation and biosynthesis of unsaturated FAs were enhanced, while in Lotus, FA synthesis genes were up-regulated during AMS. Furthermore, FAS protein localization to mitochondria suggests FA biosynthesis and elongation may also occur in AMF. Our results suggest the existence of interspecific partitioning of PL resources for generation of LPC and novel candidate bioactive PLs in the Lotus-G. intraradices symbiosis. Moreover, the data advocate research with phylogenetically diverse Glomeromycota species for a broader understanding of the molecular underpinnings of AMS. © 2015 John Wiley & Sons Ltd.

Radiation Resistant Hybrid Lotus Effect Photoelectrocatalytic Self-Cleaning Anti-Contamination Coatings, Phase I

Data.gov (United States)

National Aeronautics and Space Administration — This project will develop radiation resistant hybrid Lotus Effect photoelectrocatalytic self-cleaning anti-contamination coatings for application to Lunar...

Zizyphus lotus L. (Desf. modulates antioxidant activity and human T-cell proliferation

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Belarbi Meriem

2010-09-01

Full Text Available Abstract Background Zizyphus lotus L. (Desf. also known as Jujube, is a deciduous shrub which belongs to Rhamnaceae family. This plant is used in Algerian traditional medicine for its anti-diabetic, sedative, analgesic, anti-inflammatory and hypoglycaemic activities. In the present study, we determined the concentrations of different vitamins (vitamin A, C and E and fatty acids in root, stem, leaves, fruit pulp and seed of Zizyphus lotus L. (Desf. and assessed the effects of their aqueous extracts on antioxidant status and human T-cell proliferation. Methods Aqueous filtrates from different parts, i.e, root, leaf, stem, fruit pulp and seed, of Zizyphus lotus L. (Desf. were prepared. Vitamin C levels were determined by precipitating with 10% trichloroacetic acid and vitamin A and E were assessed by HPLC. Lipid composition of these extracts was determined by gas-liquid chromatography. Anti-oxidant capacity was evaluated by using anti-radical resistance kit [Kit Radicaux Libres (KRL@; Kirial International SA, Couternon, France]. T-cell blastogenesis was assessed by the incorporation of 3H-thymidine. IL-2 gene expression was evaluated by RT-qPCR. Results Our results show that fruit pulp contained higher vitamin A and C contents than other parts of the plant. Furthermore, the fruit pulp was the richest source of linoleic acid (18:2n-6, a precursor of n-6 fatty acids. Fruit seeds possessed higher vitamin C levels than leaves, roots and stem. The leaves were the richest source of vitamin E and linolenic acid (18:3n-3, a precursor of n-3 fatty acids. The antioxidant capacity of the different extracts, measured by KRL@ test, was as follows: pulp Zizyphus lotus L. (Desf. exerted immunosuppressive effects. Conclusion Seed extracts exerted the most potent immunosuppressive effects on T cell proliferation and IL-2 mRNA expression. The results of the present study are discussed in the light of their use to modulate the immune-mediated diseases.

Tunable hydrodynamic characteristics in microchannels with biomimetic superhydrophobic (lotus leaf replica) walls.

Science.gov (United States)

Dey, Ranabir; Raj M, Kiran; Bhandaru, Nandini; Mukherjee, Rabibrata; Chakraborty, Suman

2014-05-21

The present work comprehensively addresses the hydrodynamic characteristics through microchannels with lotus leaf replica (exhibiting low adhesion and superhydrophobic properties) walls. The lotus leaf replica is fabricated following an efficient, two-step, soft-molding process and is then integrated with rectangular microchannels. The inherent biomimetic, superhydrophobic surface-liquid interfacial hydrodynamics, and the consequential bulk flow characteristics, are critically analyzed by the micro-particle image velocimetry technique. It is observed that the lotus leaf replica mediated microscale hydrodynamics comprise of two distinct flow regimes even within the low Reynolds number paradigm, unlike the commonly perceived solely apparent slip-stick dominated flows over superhydrophobic surfaces. While the first flow regime is characterized by an apparent slip-stick flow culminating in an enhanced bulk throughput rate, the second flow regime exhibits a complete breakdown of the aforementioned laminar and uni-axial flow model, leading to a predominantly no-slip flow. Interestingly, the critical flow condition dictating the transition between the two hydrodynamic regimes is intrinsically dependent on the micro-confinement effect. In this regard, an energetically consistent theoretical model is also proposed to predict the alterations in the critical flow condition with varying microchannel configurations, by addressing the underlying biomimetic surface-liquid interfacial conditions. Hence, the present research endeavour provides a new design-guiding paradigm for developing multi-functional microfluidic devices involving biomimetic, superhydrophobic surfaces, by judicious exploitation of the tunable hydrodynamic characteristics in the two regimes.

Analysis of Flavonoids in Lotus (Nelumbo nucifera Leaves and Their Antioxidant Activity Using Macroporous Resin Chromatography Coupled with LC-MS/MS and Antioxidant Biochemical Assays

Directory of Open Access Journals (Sweden)

Ming-Zhi Zhu

2015-06-01

Full Text Available Lotus (Nelumbo nucifera leaves, a traditional Chinese medicinal herb, are rich in flavonoids. In an effort to thoroughly analyze their flavonoid components, macroporous resin chromatography coupled with HPLC-MS/MS was employed to simultaneously enrich and identify flavonoids from lotus leaves. Flavonoids extracted from lotus leaves were selectively enriched in the macroporous resin column, eluted subsequently as fraction II, and successively subjected to analysis with the HPLC-MS/MS and bioactivity assays. Altogether, fourteen flavonoids were identified, four of which were identified from lotus leaves for the first time, including quercetin 3-O-rhamnopyranosyl-(1→2-glucopyranoside, quercetin 3-O-arabinoside, diosmetin 7-O-hexose, and isorhamnetin 3-O-arabino- pyranosyl-(1→2-glucopyranoside. Further bioactivity assays revealed that these flavonoids from lotus leaves possess strong antioxidant activity, and demonstrate very good potential to be explored as food supplements or even pharmaceutical products to improve human health.

The WRKY Transcription Factor Genes in Lotus japonicus

OpenAIRE

Song, Hui; Wang, Pengfei; Nan, Zhibiao; Wang, Xingjun

2014-01-01

WRKY transcription factor genes play critical roles in plant growth and development, as well as stress responses. WRKY genes have been examined in various higher plants, but they have not been characterized in Lotus japonicus. The recent release of the L. japonicus whole genome sequence provides an opportunity for a genome wide analysis of WRKY genes in this species. In this study, we identified 61 WRKY genes in the L. japonicus genome. Based on the WRKY protein structure, L. japonicus WRKY (...

Transcriptome response mediated by cold stress in Lotus japonicus

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Pablo Ignacio Calzadilla

2016-03-01

Full Text Available Members of the Lotus genus are important as agricultural forage sources under marginal environmental conditions given their high nutritional value and tolerance of various abiotic stresses. However, their dry matter production is drastically reduced in cooler seasons, while their response to such conditions is not well studied. This paper analyzes cold acclimation of the genus by studying Lotus japonicus over a stress period of 24 h. High-throughput RNA sequencing was used to identify and classify 1077 differentially expressed genes, of which 713 were up-regulated and 364 were down-regulated. Up-regulated genes were principally related to lipid, cell wall, phenylpropanoid, sugar, and proline regulation, while down-regulated genes affected the photosynthetic process and chloroplast development. Together, a total of 41 cold-inducible transcription factors were identified, including members of the AP2/ERF, NAC, MYB, and WRKY families; two of them were described as putative novel transcription factors. Finally, DREB1/CBFs were described with respect to their cold stress expression profiles. This is the first transcriptome profiling of the model legume L. japonicus under cold stress. Data obtained may be useful in identifying candidate genes for breeding modified species of forage legumes that more readily acclimate to low temperatures

Fusion of nacre, mussel, and lotus leaf: bio-inspired graphene composite paper with multifunctional integration

Science.gov (United States)

Zhong, Da; Yang, Qinglin; Guo, Lin; Dou, Shixue; Liu, Kesong; Jiang, Lei

2013-06-01

Multifunctional integration is an inherent characteristic for biological materials with multiscale structures. Learning from nature is an effective approach for scientists and engineers to construct multifunctional materials. In nature, mollusks (abalone), mussels, and the lotus have evolved different and optimized solutions to survive. Here, bio-inspired multifunctional graphene composite paper was fabricated in situ through the fusion of the different biological solutions from nacre (brick-and-mortar structure), mussel adhesive protein (adhesive property and reducing character), and the lotus leaf (self-cleaning effect). Owing to the special properties (self-polymerization, reduction, and adhesion), dopamine could be simultaneously used as a reducing agent for graphene oxide and as an adhesive, similar to the mortar in nacre, to crosslink the adjacent graphene. The resultant nacre-like graphene paper exhibited stable superhydrophobicity, self-cleaning, anti-corrosion, and remarkable mechanical properties underwater.Multifunctional integration is an inherent characteristic for biological materials with multiscale structures. Learning from nature is an effective approach for scientists and engineers to construct multifunctional materials. In nature, mollusks (abalone), mussels, and the lotus have evolved different and optimized solutions to survive. Here, bio-inspired multifunctional graphene composite paper was fabricated in situ through the fusion of the different biological solutions from nacre (brick-and-mortar structure), mussel adhesive protein (adhesive property and reducing character), and the lotus leaf (self-cleaning effect). Owing to the special properties (self-polymerization, reduction, and adhesion), dopamine could be simultaneously used as a reducing agent for graphene oxide and as an adhesive, similar to the mortar in nacre, to crosslink the adjacent graphene. The resultant nacre-like graphene paper exhibited stable superhydrophobicity, self

SRAP analysis of M3 lotus mutants induced by Fe+ ion implantation

International Nuclear Information System (INIS)

Jia Yanyan; Deng Chuanliang; Gao Jun; Ren Yingxue; Wang Ningna; Gao Wujun; Lu Longdou; Zhang Tao; Li Pengfei

2011-01-01

To examine and determine the lotus mutants induced by the same Fe + ion implantation at the molecular level, the SRAP technique was used, and the non denatured polyacrylamide gel electrophoresis was undertaken to analyze the PCR products. At the optimized SRAP reaction condition, of the 121 primer pairs tested, 10 primer pairs could amplify stable and remarkable specific bands, with primer polymorphism of 8.26%. This 10 primer pairs amplified a total of 215 bands, 83 of which were polymorphic, and the percent of polymorphic bands was 38.6%. A total of 141 bands were amplified for the mutant 1, 22 of which were different from the control with the variation ratio of 15.6%. However, the variation ratios of mutant 2 to 6 were 16.4%, 17.1%, 16.9%, 18.2% and 20.5% respectively. The results indicated that Fe + ion implantation into the seeds of Baiyangdian red lotus could induce random genetic DNA variations. (authors)

GC-EI-MS identification data of neutral sugars of polysaccharides extracted from Zizyphus lotus fruit

Directory of Open Access Journals (Sweden)

Khaoula Mkadmini Hammi

2018-06-01

Full Text Available Gas chromatography coupled to mass spectrometer (GC–MS was used to identify and to quantify neutral sugars that constitute the water soluble polysaccharides from Zizyphus lotus fruit. The trimethylsilyl (TMS method was successfully used for derivatization of the monosaccharides units of extracted polysaccharides that were released by hydrolysis method. Sugars were identified based on their retention times compared with those of standards and the NIST MS Spectral Library. All sugars were quantified in TIC (Total Ion Current mode using calibration curves. Data is related to “Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity” (Mkadmini Hammi et al., 2016 [1]. Keywords: Trimethylsilyl, Derivatization, GC–MS, Neutral sugar

A simple procedure for the isolation of L-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus.

Science.gov (United States)

Allen, H J; Johnson, E A

1977-10-01

L-Fucose-binding lectins from Ulex europeaus and Lotus tetragonolobus were isolated by affinity chromatography on columns of L-fucose-Sepharose 6B. L-Fucose was coupled to Sepharose 6B after divinyl sulfone-activation of the gel to give an affinity adsorbent capable of binding more than 1.2 mg of Ulex lextin/ml of gel, which could then be eluted with 0.1M or 0.05M L-fucose. Analysis of the isolated lectins by hemagglutination assay, by gel filtration, and polyacrylamide disc-electrophoresis revealed the presence of isolectins, or aggregated species, or both. The apparent mol. wt. of the major lectin fraction from Lotus was 35000 when determined on Sephadex G-200 or Ultrogel AcA 34. In contrast, the apparent mol. wt. of the major lectin fraction from Ulex was 68 000 when chromatographed on Sephadex G-200 and 45 000 when chromatographed on Ultrogel AcA 34. The yields of lectins were 4.5 mg/100 g of Ulex seeds and 394 mg/100 g of Lotus seeds.

Interfacing COTS Speech Recognition and Synthesis Software to a Lotus Notes Military Command and Control Database

Science.gov (United States)

Carr, Oliver

2002-10-01

Speech recognition and synthesis technologies have become commercially viable over recent years. Two current market leading products in speech recognition technology are Dragon NaturallySpeaking and IBM ViaVoice. This report describes the development of speech user interfaces incorporating these products with Lotus Notes and Java applications. These interfaces enable data entry using speech recognition and allow warnings and instructions to be issued via speech synthesis. The development of a military vocabulary to improve user interaction is discussed. The report also describes an evaluation in terms of speed of the various speech user interfaces developed using Dragon NaturallySpeaking and IBM ViaVoice with a Lotus Notes Command and Control Support System Log database.

Molecular Structure-Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method.

Science.gov (United States)

Tang, Xiaosheng; Tang, Ping; Liu, Liangliang

2017-06-23

Lotus leaf has gained growing popularity as an ingredient in herbal formulations due to its various activities. As main functional components of lotus leaf, the difference in structure of flavonoids affected their binding properties and activities. In this paper, the existence of 11 flavonoids in lotus leaf extract was confirmed by High Performance Liquid Chromatography (HPLC) analysis and 11 flavonoids showed various contents in lotus leaf. The interactions between lotus leaf extract and two kinds of serum albumins (human serum albumin (HSA) and bovine serum albumin (BSA)) were investigated by spectroscopic methods. Based on the fluorescence quenching, the interactions between these flavonoids and serum albumins were further checked in detail. The relationship between the molecular properties of flavonoids and their affinities for serum albumins were analyzed and compared. The hydroxylation on 3 and 3' position increased the affinities for serum albumins. Moreover, both of the methylation on 3' position of quercetin and the C₂=C₃ double bond of apigenin and quercetin decreased the affinities for HSA and BSA. The glycosylation lowered the affinities for HSA and BSA depending on the type of sugar moiety. It revealed that the hydrogen bond force played an important role in binding flavonoids to HSA and BSA.

Detecting Adulteration in Lotus Honey Using a Machine Olfactory System

Directory of Open Access Journals (Sweden)

M Hajinezhad

2017-10-01

Full Text Available Introduction Honey is a supersaturated sugar and viscose solution taken from the nectar of flowers, collected and modified by honeybees. Many producers of honey add some variety of sugars in honey that make difficulties with detection of adulterated and pure honey. Flavor is one of the most important parameters in the classification of honey samples and the smell emitted by the honey depending on the different flowers and constituents that could be different. This causes using an electronic nose system to detect honey adulteration. Materials and Methods Honey samples used in this study were lotus honey that was supplied from a market in Karaj city, Alborz province, Iran. Adulterated honey, along with percentages of fraud (by weight of zero, 20, 35 and 50 percent, was prepared by mixing sugar syrup. Each group of samples, nine times were tested by the electronic nose system. The proposed system, consists of six metal oxide semiconductor sensors, sensor chamber, sample chamber, data acquisition systems, power supply, electric valves, and pumps. Electronic nose is planned for three-phase system baseline correction, the smell of sample injection and cleaning of the sensor and sample chambers with clean air (Oxygen. Responses of the sensors were collected and stored in 420 seconds by a data acquisition system and LabView ver 2012 software. We used fractional method in this study, in order to improve the quality of the information available and to optimize the array output before passing it on to the pattern recognition system. Linear Discriminant Analysis (LDA, Principal Component Analysis (PCA and Artificial neural network (ANN were the methods used for analyzing and recognizing pattern of electronic nose signals. Data processing was carried out using Microsoft Excel, neuralsolution 5 and Unscrambler X 10.3 (CAMO AS, Norway. Results and Discussion PCA Results PCA reduces the complexity of the data-set and is performed with no information on the

Identification and Functional Characterisation of Nod Factor Receptor (NFR) Paralogs in Lotus japonicus

DEFF Research Database (Denmark)

Vestergaard, Gitte; Radutoiu, Elena Simona; Stougaard, Jens

an important missing link in plant-bacterial communication. This picture changed with the cloning of LysM-domain containing receptor-like kinases (LysM-RLKs) in different legume species. In Lotus japonicus, two LysM-RLKs, Nod Factor Receptor 1 (NFR1) and Nod Factor Receptor 5 (NFR5), are believed to bind Nod...... using the sequences of NFR1 and NFR5. Microsattelite markers were developed from each TAC clone containing the LysM-RLK, permitting us to locate the genes on a genetic map of Lotus japonicus. In order to get more insight into the function of these genes an inverse genetic approach using RNAi has been...

Polyphenols from Ziziphus lotus grown in Tunesia

International Nuclear Information System (INIS)

Qa'dan, F.

2003-01-01

Four monomeric and four oligomeric flavanoids have been identified from the root bark of Zizphus lotus traditionally used for the treatment of diabetes in Tunisian traditional medicine. Flavan-3-oles are (-)-epicatechin(1), (+)-catechin (2), (-)-epigallocatechin (3) and (+)-gallocatechin (4); proanthcyanidins are epicatechin-(4??8)-catechin (procyanidin B-1,5), catechin-(4??8)-catechin (procyanidin B-3, 6), catechin-(4??8)-epicatechin ( procyanidin B-4, 7) and gallocatechin-(4??8)-gallocatechin (prodelphinidin B-3, 8) were isolated. The reported compounds were established as their peracetate derivatives, on the basis of their chemical and spectral evidences. The spectral data of the peracetate derivative of the prodelphinidin(8a) are reported. (author) 19 refs

Expression of a complete soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus

DEFF Research Database (Denmark)

Stougaard, J; Petersen, T E; Marcker, K A

1987-01-01

The complete soybean leghemoglobin lbc(3) gene was transferred into the legume Lotus corniculatus using an Agrobacterium rhizogenes vector system. Organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with Rhizobium loti...

Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn

Science.gov (United States)

Chen, Sha; Xiang, Yue; Deng, Jiao; Liu, Yanling; Li, Shaohua

2013-01-01

A validated HPLC-DAD-ESI-MSn method for the analysis of non-anthocyanin flavonoids was applied to nine different tissues of twelve lotus genotypes of Nelumbo nucifera and N. lutea, together with an optimized anthocyanin extraction and separation protocol for lotus petals. A total of five anthocyanins and twenty non-anthocyanin flavonoids was identified and quantified. Flavonoid contents and compositions varied with cultivar and tissue and were used as a basis to divide tissues into three groups characterized by kaempferol and quercetin derivatives. Influences on flower petal coloration were investigated by principal components analyses. High contents of kaempferol glycosides were detected in the petals of N. nucifera while high quercetin glycoside concentrations occurred in N. lutea. Based on these results, biosynthetic pathways leading to specific compounds in lotus tissues are deduced through metabolomic analysis of different genotypes and tissues and correlations among flavonoid compounds. PMID:23646125

Changes in physicochemical properties related to the texture of lotus rhizomes subjected to heat blanching and calcium immersion.

Science.gov (United States)

Zhao, Wenlin; Xie, Wei; Du, Shenglan; Yan, Shoulei; Li, Jie; Wang, Qingzhang

2016-11-15

Pretreatments such as low temperature blanching and/or calcium soaking affect the cooked texture of vegetal food. In the work, lotus rhizomes (Nelumbo nucifera Gaertn.) were pretreated using the following 4 treatments, blanching at 40°C, blanching at 90°C, soaking in 0.5% CaCl2, and blanching at 40°C followed by immersion in 0.5% CaCl2. Subsequently, the cell wall material of pretreated samples was isolated and fractioned to identify changes in the degree of esterification (DE) and monosaccharide content of each section, and the texture of the lotus rhizomes in different pre-treatments was determined after thermal processing with different time. The results showed that the greatest hardness was obtained after blanching at 40°C in CaCl2, possibly attributing to the formation of a pectate calcium network, which maintains the integrity of cell walls. Furthermore, the content of galactose, rhamnose and arabinose decreased due to the breakage of sugar backbones and subsequent damage to cell walls. Our results may provide a reference for lotus rhizome processing. Copyright © 2016 Elsevier Ltd. All rights reserved.

3D Printing of Lotus Root-Like Biomimetic Materials for Cell Delivery and Tissue Regeneration.

Science.gov (United States)

Feng, Chun; Zhang, Wenjie; Deng, Cuijun; Li, Guanglong; Chang, Jiang; Zhang, Zhiyuan; Jiang, Xinquan; Wu, Chengtie

2017-12-01

Biomimetic materials have drawn more and more attention in recent years. Regeneration of large bone defects is still a major clinical challenge. In addition, vascularization plays an important role in the process of large bone regeneration and microchannel structure can induce endothelial cells to form rudimentary vasculature. In recent years, 3D printing scaffolds are major materials for large bone defect repair. However, these traditional 3D scaffolds have low porosity and nonchannel structure, which impede angiogenesis and osteogenesis. In this study, inspired by the microstructure of natural plant lotus root, biomimetic materials with lotus root-like structures are successfully prepared via a modified 3D printing strategy. Compared with traditional 3D materials, these biomimetic materials can significantly improve in vitro cell attachment and proliferation as well as promote in vivo osteogenesis, indicating potential application for cell delivery and bone regeneration.

Fusion of nacre, mussel, and lotus leaf: bio-inspired graphene composite paper with multifunctional integration.

Science.gov (United States)

Zhong, Da; Yang, Qinglin; Guo, Lin; Dou, Shixue; Liu, Kesong; Jiang, Lei

2013-07-07

Multifunctional integration is an inherent characteristic for biological materials with multiscale structures. Learning from nature is an effective approach for scientists and engineers to construct multifunctional materials. In nature, mollusks (abalone), mussels, and the lotus have evolved different and optimized solutions to survive. Here, bio-inspired multifunctional graphene composite paper was fabricated in situ through the fusion of the different biological solutions from nacre (brick-and-mortar structure), mussel adhesive protein (adhesive property and reducing character), and the lotus leaf (self-cleaning effect). Owing to the special properties (self-polymerization, reduction, and adhesion), dopamine could be simultaneously used as a reducing agent for graphene oxide and as an adhesive, similar to the mortar in nacre, to crosslink the adjacent graphene. The resultant nacre-like graphene paper exhibited stable superhydrophobicity, self-cleaning, anti-corrosion, and remarkable mechanical properties underwater.

Identification and antioxidant properties of polyphenols in lotus seed epicarp at different ripening stages.

Science.gov (United States)

Liu, Ying; Ma, Shuang-shuang; Ibrahim, S A; Li, Er-hu; Yang, Hong; Huang, Wen

2015-10-15

In this study, polyphenols from lotus seed epicarp (PLSE) at three different ripening stages were purified by column chromatography and identified by RP-HPLC and HPLC-ESI-MS(2). The antioxidant activities of PLSE were also investigated. We found that the contents of PLSE at the green ripening stage, half ripening stage and full ripening stage are 13.08%, 10.95% and 6.73% respectively. The levels of catechin, epicatechin, hyperoside, and isoquercitrin in PLSE at the three different ripening stages were different. Moreover, the amounts of catechin and epicatechin decreased, while the contents of hyperoside and isoquercitrin increased as the seed ripened. We found that PLSE at three different ripening stages had good scavenging abilities on DPPH and ABTS(+) radicals. However, the scavenging ability decreased with maturation. Our results may be valuable with regard to the utilization of lotus seed epicarp as a functional food material. Copyright © 2015 Elsevier Ltd. All rights reserved.

Influence of gamma-radiation on the nutritional and functional qualities of lotus seed flour.

Science.gov (United States)

Bhat, Rajeev; Sridhar, Kandikere Ramaiah; Karim, Alias A; Young, Chiu C; Arun, Ananthapadmanabha B

2009-10-28

In the present study, we investigated the physicochemical and functional properties of lotus seed flour exposed to low and high doses of gamma-radiation (0-30 kGy; the dose recommended for quarantine and hygienic purposes). The results indicated raw seed flour to be rich in nutrients with minimal quantities of antinutritional factors. Irradiation resulted in a dose-dependent increase in some of the proximal constituents. The raw and gamma-irradiated seeds meet the Food and Agricultural Organization-World Health Organization recommended pattern of essential amino acids. Some of the antinutritional factors (phytic acid, total phenolics, and tannins) were lowered with gamma-irradiation, while the seed flours were devoid of lectins, L-3,4-dihydroxyphenylalanine, and polonium-210. The functional properties of the seed flour were significantly improved with gamma-radiation. gamma-radiation selectively preserved or improved the desired nutritional and functional traits of lotus seeds, thus ensuring a safe production of appropriate nutraceutically valued products.

Morphological evolution of TiO2 nanotube arrays with lotus-root-shaped nanostructure

Science.gov (United States)

Yu, Dongliang; Song, Ye; Zhu, Xufei; Yang, Ruiquan; Han, Aijun

2013-07-01

TiO2 nanotube arrays (TNAs) with lotus-root-shaped nanostructure have been fabricated by a modified two-step electrochemical anodization method. In the present work, different morphologies formed under different anodizing voltages are investigated in detail by field-emission scanning electron microscope. The results show that the concaves left by the first-step anodization can guide the uniform growth of TNAs in some degree as the second-step anodizing voltage is the same with that in the first step, however, when lower voltages are adopted in the second-step anodization, no guidance can be achieved, and different morphological TNAs with lotus-root-shaped nanostructure are fabricated. And we find that the nanotube diameters are directly proportional to the applied voltage in the second-step anodization. Furthermore, a possible mechanism for the growth of the TiO2 nanotubes with the special morphology is proposed for the first time, which depends on both the oxygen bubble mold and the viscous flow of the barrier oxide from the pore base to the pore wall.

Biomimetic approaches for green tribology: from the lotus effect to blood flow control

International Nuclear Information System (INIS)

Maani, Nazanin; Rayz, Vitaliy S; Nosonovsky, Michael

2015-01-01

The research in Green tribology combines several areas including biomimetic tribomaterials and surfaces for controlled adhesion. Biomimetic surfaces mimic living nature and thus they are eco-friendly. The most famous biomimetic surface effect is the Lotus effect (reduction of water adhesion to a solid surface due to micro/nanostructuring of the solid surface). Several extensions of the Lotus effect have been discussed in the literature including the oleophobicity (repelling organic liquids such as oils), underwater oleophobicity to reduce fouling, and the shark skin effect (flow drag reduction due to specially oriented micro-riblets). Here we suggest a potentially important application of micro/nanostructured surfaces in the biomedical area: the micro/nanostructure controlled adhesion in blood flow. Blood is a suspension, and its adhesion properties are different from those of water and oil. For many cardiovascular applications, it is desirable to reduce stagnation and clotting of blood. Therefore, both the underwater oleophobicuity and shark-skin effect can be used. We discuss how computational fluid dynamics models can be used to investigate the structure–property relationships of surface pattern-controlled blood flow adhesion. (paper)

Genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain R7A.

Science.gov (United States)

Kelly, Simon; Sullivan, John; Ronson, Clive; Tian, Rui; Bräu, Lambert; Munk, Christine; Goodwin, Lynne; Han, Cliff; Woyke, Tanja; Reddy, Tatiparthi; Huntemann, Marcel; Pati, Amrita; Mavromatis, Konstantinos; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Reeve, Wayne

2014-01-01

Mesorhizobium loti strain R7A was isolated in 1993 in Lammermoor, Otago, New Zealand from a Lotus corniculatus root nodule and is a reisolate of the inoculant strain ICMP3153 (NZP2238) used at the site. R7A is an aerobic, Gram-negative, non-spore-forming rod. The symbiotic genes in the strain are carried on a 502-kb integrative and conjugative element known as the symbiosis island or ICEMlSym(R7A). M. loti is the microsymbiont of the model legume Lotus japonicus and strain R7A has been used extensively in studies of the plant-microbe interaction. This report reveals that the genome of M. loti strain R7A does not harbor any plasmids and contains a single scaffold of size 6,529,530 bp which encodes 6,323 protein-coding genes and 75 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

3D Printing of Lotus Root‐Like Biomimetic Materials for Cell Delivery and Tissue Regeneration

Science.gov (United States)

Feng, Chun; Zhang, Wenjie; Deng, Cuijun; Li, Guanglong; Chang, Jiang; Zhang, Zhiyuan

2017-01-01

Abstract Biomimetic materials have drawn more and more attention in recent years. Regeneration of large bone defects is still a major clinical challenge. In addition, vascularization plays an important role in the process of large bone regeneration and microchannel structure can induce endothelial cells to form rudimentary vasculature. In recent years, 3D printing scaffolds are major materials for large bone defect repair. However, these traditional 3D scaffolds have low porosity and nonchannel structure, which impede angiogenesis and osteogenesis. In this study, inspired by the microstructure of natural plant lotus root, biomimetic materials with lotus root‐like structures are successfully prepared via a modified 3D printing strategy. Compared with traditional 3D materials, these biomimetic materials can significantly improve in vitro cell attachment and proliferation as well as promote in vivo osteogenesis, indicating potential application for cell delivery and bone regeneration. PMID:29270348

Proteome reference maps of the Lotus japonicus nodule and root

DEFF Research Database (Denmark)

Dam, Svend Secher; Dyrlund, Thomas F.; Ussatjuk, Anna

2014-01-01

formation mutant (snf1) was determined. From nodules and roots, 780 and 790 protein spots from 2D gels were identified and approximately 45% of the corresponding unique gene accessions were common. Including a previous proteomics set from Lotus pod and seed, the common gene accessions were decreased to 7...... stress level at this developmental stage. In contrast, protein spots corresponding to nodulins such as leghemoglobin, asparagine synthetase, sucrose synthase, and glutamine synthetase were prevalent in red nodules. The distinct biochemical state of nodules was further highlighted by the conspicuous...

Lotus corniculatus Crop Growth of in Crude Oil Contaminated Soil. Part 2 Biomass Metals Bioaccumulation

Directory of Open Access Journals (Sweden)

Florica Morariu

2016-05-01

Full Text Available Phytoremediation involves the ability of plants to remove pollutants and is a promise on low costs and efficient processes for cleaning oil polluted soil. Studies for phytoremediation of soils polluted with petroleum products were critical and were based on monitoring strategies implemented efficiency. These strategies are based on the necessity of treating polluted soil and plant cultivation. Treatment was performed with recycled materials, sewage sludge as fertilizer and fly ash as amendment. The studies took on the characteristics of qualitative and quantitative of Lotus corniculatus crops, plants tolerant to conditions for phytoremediation strategy implemented on polluted soils by 80.5 ± 3.9 g·kg-1 D.M. The use of sewage sludge mixed with fly ash resulted in formation of a layer covering the surface with vegetable grown by 85 - 94 % in July and by 67 - 83 % in August. In Lotus corniculatus crops have not been registered bioaccumulation of toxic metals according to legislation from Romania.

Morphological evolution of TiO{sub 2} nanotube arrays with lotus-root-shaped nanostructure

Energy Technology Data Exchange (ETDEWEB)

Yu, Dongliang; Song, Ye; Zhu, Xufei, E-mail: zhuxufei.njust@163.com; Yang, Ruiquan; Han, Aijun

2013-07-01

TiO{sub 2} nanotube arrays (TNAs) with lotus-root-shaped nanostructure have been fabricated by a modified two-step electrochemical anodization method. In the present work, different morphologies formed under different anodizing voltages are investigated in detail by field-emission scanning electron microscope. The results show that the concaves left by the first-step anodization can guide the uniform growth of TNAs in some degree as the second-step anodizing voltage is the same with that in the first step, however, when lower voltages are adopted in the second-step anodization, no guidance can be achieved, and different morphological TNAs with lotus-root-shaped nanostructure are fabricated. And we find that the nanotube diameters are directly proportional to the applied voltage in the second-step anodization. Furthermore, a possible mechanism for the growth of the TiO{sub 2} nanotubes with the special morphology is proposed for the first time, which depends on both the oxygen bubble mold and the viscous flow of the barrier oxide from the pore base to the pore wall.

Damage and failure modeling of lotus-type porous material subjected to low-cycle fatigue

Directory of Open Access Journals (Sweden)

J. Kramberger

2016-01-01

Full Text Available The investigation of low-cycle fatigue behaviour of lotus-type porous material is presented in this paper. Porous materials exhibit some unique features which are useful for a number of various applications. This paper evaluates a numerical approach for determining of damage initiation and evolution of lotus-type porous material with computational simulations, where the considered computational models have different pore topology patterns. The low-cycle fatigue analysis was performed by using a damage evolution law. The damage state was calculated and updated based on the inelastic hysteresis energy for stabilized cycle. Degradation of the elastic stifness was modeled using scalar damage variable. In order to examine crack propagation path finite elements with severe damage were deleted and removed from the mesh during simulation. The direct cyclic analysis capability in Abaqus/Standard was used for low-cycle fatigue analysis to obtain the stabilized response of a model subjected to the periodic loading. The computational results show a qualitative understanding of pores topology influence on low-cycle fatigue under transversal loading conditions in relation to pore orientation.

Efficacy and safety of the Lotus Valve System for treatment of patients with severe aortic valve stenosis and intermediate surgical risk

DEFF Research Database (Denmark)

De Backer, Ole; Götberg, Matthias; Ihlberg, Leo

2016-01-01

increasingly used to treat patients with an intermediate risk profile. METHODS AND RESULTS: The study was designed as an independent Nordic multicenter registry of intermediate risk patients treated with the Lotus Valve System (Boston Scientific, MA, USA; N=154). Valve Academic Research Consortium (VARC......)-defined device success was obtained in 97.4%. A Lotus Valve was successfully implanted in all patients. There was no valve migration, embolization, ectopic valve deployment, or TAV-in-TAV deployment. The VARC-defined combined safety rate at 30days was 92.2%, with a mortality rate of 1.9% and stroke rate of 3...

Biomimetic fabrication and tunable wetting properties of three-dimensional hierarchical ZnO structures by combining soft lithography templated with lotus leaf and hydrothermal treatments

OpenAIRE

Dai, Shuxi; Zhang, Dianbo; Shi, Qing; Han, Xiao; Wang, Shujie; Du, Zuliang

2013-01-01

Three-dimensional hierarchical ZnO films with lotus-leaf-like micro/nano structures were successfully fabricated via a biomimetic route combining sol-gel technique, soft lithography and hydrothermal treatments. PDMS mold replicated from a fresh lotus leaf was used to imprint microscale pillar structures directly into a ZnO sol film. Hierarchical ZnO micro/nano structures were subsequently fabricated by a low-temperature hydrothermal growth of secondary ZnO nanorod arrays on the micro-structur...

Polycyclopentene-Crystal-Decorated Carbon Nanotubes by Convenient Large-Scale In Situ Polymerization and their Lotus-Leaf-Like Superhydrophobic Films.

Science.gov (United States)

Xu, Lixin; Huang, Lingqi; Ye, Zhibin; Meng, Nan; Shu, Yang; Gu, Zhiyong

2017-02-01

In situ Pd-catalyzed cyclopentene polymerization in the presence of multi-walled carbon nanotubes (MWCNTs) is demonstrated to effectively render, on a large scale, polycyclopentene-crystal-decorated MWCNTs. Controlling the catalyst loading and/or time in the polymerization offers a convenient tuning of the polymer content and the morphology of the decorated MWCNTs. Appealingly, films made of the decorated carbon nanotubes through simple vacuum filtration show the characteristic lotus-leaf-like superhydrophobicity with high water contact angle (>150°), low contact angle hysteresis (<10°), and low water adhesion, while being electrically conductive. This is the first demonstration of the direct fabrication of lotus-leaf-like superhydrophobic films with solution-grown polymer-crystal-decorated carbon nanotubes. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

EELS data acquisition, processing and display for the Zeiss CEM 902 based on LOTUS 1-2-3: application examples from a biological system and inorganic transition metal compounds.

Science.gov (United States)

Drechsler, M; Cantow, H J

1991-04-01

A personal computer combined with LOTUS 1-2-3 software, including the RS232 module of LOTUS MEASURE and a 12-bit ADC, has been used for data acquisition of electron energy-loss spectroscopy (EELS) spectra with the Zeiss CEM 902. The internal macro language of LOTUS 1-2-3 allows a menu-driven procedure. Macro-programs partly combined with external FORTRAN programs can be chosen from the menu for background subtraction, removal of multiple scattering effects by deconvolution, elemental quantification and several utilities. For special applications or conditions the macro programs can easily be modified. Spectra from crystals of two inorganic transition metal compounds, ruthenium trichloride and vanadium disulphide, and from a biological sample are presented as examples of the application of this software.

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities.

Science.gov (United States)

Zgadzaj, Rafal; Garrido-Oter, Ruben; Jensen, Dorthe Bodker; Koprivova, Anna; Schulze-Lefert, Paul; Radutoiu, Simona

2016-12-06

Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive nodule- and root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance.

Conduction Abnormalities and Permanent Pacemaker Implantation After Transcatheter Aortic Valve Replacement Using the Repositionable LOTUS Device: The United Kingdom Experience.

Science.gov (United States)

Rampat, Rajiv; Khawaja, M Zeeshan; Hilling-Smith, Roland; Byrne, Jonathan; MacCarthy, Philip; Blackman, Daniel J; Krishnamurthy, Arvindra; Gunarathne, Ashan; Kovac, Jan; Banning, Adrian; Kharbanda, Raj; Firoozi, Sami; Brecker, Stephen; Redwood, Simon; Bapat, Vinayak; Mullen, Michael; Aggarwal, Suneil; Manoharan, Ganesh; Spence, Mark S; Khogali, Saib; Dooley, Maureen; Cockburn, James; de Belder, Adam; Trivedi, Uday; Hildick-Smith, David

2017-06-26

The authors report the incidence of pacemaker implantation up to hospital discharge and the factors influencing pacing rate following implantation of the LOTUS bioprosthesis (Boston Scientific, Natick, Massachusetts) in the United Kingdom. Transcatheter aortic valve replacement (TAVR) is associated with a significant need for permanent pacemaker implantation. Pacing rates vary according to the device used. The REPRISE II (Repositionable Percutaneous Replacement of Stenotic Aortic Valve Through Implantation of Lotus Valve System) trial reported a pacing rate of 29% at 30 days after implantation of the LOTUS device. Data were collected retrospectively on 228 patients who had the LOTUS device implanted between March 2013 and February 2015 across 10 centers in the United Kingdom. Twenty-seven patients (12%) had pacemakers implanted pre-procedure and were excluded from the analysis. Patients were aged 81.2 ± 7.7 years; 50.7% were male. The mean pre-procedural QRS duration was 101.7 ± 20.4 ms. More than one-half of the cohort (n = 111, 55%) developed new left bundle branch block (LBBB) following the procedure. Permanent pacemakers were implanted in 64 patients (32%) with a median time to insertion of 3.0 ± 3.4 days. Chief indications for pacing were atrioventricular (AV) block (n = 46, 72%), or LBBB with 1st degree AV block (n = 11, 17%). Amongst those who received a pacemaker following TAVR the pre-procedural electrocardiogram findings included: No conduction disturbance (n = 41, 64%); 1st degree AV block (n = 10, 16%); right bundle branch block (n = 6, 9%) and LBBB (n = 5, 8%). LBBB (but not permanent pacemaker) occurred more frequently in patients who had balloon aortic valvuloplasty before TAVR (odds ratio [OR]: 1.25; p = 0.03). Pre-procedural conduction abnormality (composite of 1st degree AV block, hemiblock, right bundle branch block, LBBB) was independently associated with the need for permanent pacemaker (OR: 2.54; p = 0.048). The absence of

Microbial quality evaluation and effective decontamination of nutraceutically valued lotus seeds by electron beams and gamma irradiation

International Nuclear Information System (INIS)

Bhat, Rajeev; Sridhar, K.R.; Karim, A.A.

2010-01-01

Lotus seeds are nutraceutically valued natural plant produce, which succumbs to microbial contamination, predominantly to toxigenic moulds. Results of the present study revealed seed coat portion to harbor higher proportion of microbial load, particularly fungi than cotyledon portion. Among the mycotoxins analyzed, aflatoxins (B 1 , B 2 , G 1 and G 2 ) were below detectable limits, while the seeds were devoid of Ochratoxin-A (OTA). Application of different doses of electron beam and gamma irradiation (0, 2.5, 5, 7.5, 10, 15 and 30 kGy) for decontamination purpose revealed significant dose-dependent decrease in the fungal contaminants (P<0.05). However, the contaminant yeasts could survive up to 10 kGy dose, which could be completely eliminated at 15 kGy. From the results obtained, a dose range between 10 and 15 kGy is recommended for complete decontamination, as these doses have also been shown earlier to have minimal effects on nutritional and functional properties of lotus seeds.

Pollination success of Lotus corniculatus (L.) in an urban context

Science.gov (United States)

Pellissier, Vincent; Muratet, Audrey; Verfaillie, Fabien; Machon, Nathalie

2012-02-01

Most anthropogenic activities are known to have deleterious effects on pollinator communities. However, little is known about the influence of urbanization on pollination ecosystem services. Here, we assessed the pollination service on Lotus corniculatus (L.), a self-sterile, strictly entogamous Fabaceae commonly observed in urban and suburban areas. We assessed the pollination success of artificial Lotus corniculatus populations at three levels: at large scale, along an urbanization gradient; at intermediate scale, based on landscape fragmentation within a 250 m radius and at local scale based on floral resource abundance and local habitat type. The main findings were that the pollination success, when assessed with the number of fruit produced per inflorescence, was lower in urban areas than in suburban ones, and was negatively affected by the number of impervious spaces in the neighborhood. The relationship between the number of fruits and the distance to the nearest impervious space was either positive or negative, depending on the gray/green ratio (low vs. high). Finally, on a local scale, floral resource abundance had a negative effect on pollination success when L. corniculatus populations were located in paved courtyards, and a positive one when they were located in parks. Pollination success seems to be explained by two intertwined gradients: landscape fragmentation estimated by the number of impervious spaces in a 250 m radius around L. corniculatus populations, and the behavior of bumblebees toward birdsfoot trefoil and floral displays, which appears to differ depending on whether a neighborhood is densely or sparsely urbanized. An abundance of attracting floral resources seems to enhance pollination success for L. corniculatus if it is not too isolated from other green spaces. These results have important implications for the sustainability of pollination success in towns by identifying local and landscape factors that influence reproductive success of

76 FR 33406 - Lotus Cars Ltd. Receipt of Petition for Renewal of Temporary Exemption From the Advanced Air Bag...

Science.gov (United States)

2011-06-08

... substantial economic hardship and that it has tried in good faith to comply with the standard. In recognition... market share of its entry-level model to other brands. Lotus also alleges that it has made a good faith...

Nanocrystalline nickel films with lotus leaf texture for superhydrophobic and low friction surfaces

Science.gov (United States)

Shafiei, Mehdi; Alpas, Ahmet T.

2009-11-01

Nanostructured Ni films with high hardness, high hydrophobicity and low coefficient of friction (COF) were fabricated. The surface texture of lotus leaf was replicated using a cellulose acetate film, on which a nanocrystalline (NC) Ni coating with a grain size of 30 ± 4 nm was electrodeposited to obtain a self-sustaining film with a hardness of 4.42 GPa. The surface texture of the NC Ni obtained in this way featured a high density (4 × 10 3 mm -2) of conical protuberances with an average height of 10.0 ± 2.0 μm and a tip radius of 2.5 ± 0.5 μm. This structure increased the water repellency and reduced the COF, compared to smooth NC Ni surfaces. The application of a short-duration (120 s) electrodeposition process that deposited "Ni crowns" with a larger radius of 6.0 ± 0.5 μm on the protuberances, followed by a perfluoropolyether (PFPE) solution treatment succeeded in producing a surface texture consisting of nanotextured protuberances that resulted in a very high water contact angle of 156°, comparable to that of the superhydrophobic lotus leaf. Additionally, the microscale protuberances eliminated the initial high COF peaks observed when smooth NC Ni films were tested, and the PFPE treatment resulted in a 60% reduction in the steady-state COFs.

The trehalose utilization gene thuA ortholog in Mesorhizobium loti does not influence competitiveness for nodulation on Lotus spp.

Science.gov (United States)

Ampomah, Osei Yaw; Jensen, John Beck

2014-03-01

Competitiveness for nodulation is a desirable trait in rhizobia strains used as inoculant. In Sinorhizobium meliloti 1021 mutation in either of the trehalose utilization genes thuA or thuB influences its competitiveness for root colonization and nodule occupancy depending on the interacting host. We have therefore investigated whether mutation in the thuA ortholog in Mesorhizobium loti MAFF303099 also leads to a similar competitive phenotype on its hosts. The results show that M. loti thuA mutant Ml7023 was symbiotically effective and was as competitive as the wild type in colonization and nodule occupancy on Lotus corniculatus and Lotus japonicus. The thuA gene in M. loti was not induced during root colonization or in the infection threads unlike in S. meliloti, despite its induction by trehalose and high osmolarity in in vitro assays.

Identification of QTLs for shoot and root growth under ionic-osmotic stress in Lotus, using a RIL population

DEFF Research Database (Denmark)

Quero, Gastón; Gutíerrez, Lucía; Lascano, Ramiro

2014-01-01

The genus Lotus includes a group of forage legume species including genotypes of agronomic interest and model species. In this work, an experimental hydroponic growth system allowed the discrimination of growth responses to ionic-osmotic stress in a population of recombinant inbred lines (RILs...

Predictors of Paravalvular Regurgitation After Implantation of the Fully Repositionable and Retrievable Lotus Transcatheter Aortic Valve (from the REPRISE II Trial Extended Cohort).

Science.gov (United States)

Blackman, Daniel J; Meredith, Ian T; Dumonteil, Nicolas; Tchétché, Didier; Hildick-Smith, David; Spence, Mark S; Walters, Darren L; Harnek, Jan; Worthley, Stephen G; Rioufol, Gilles; Lefèvre, Thierry; Houle, Vicki M; Allocco, Dominic J; Dawkins, Keith D

2017-07-15

Paravalvular leak (PVL) after transcatheter aortic valve replacement is associated with worse long-term outcomes. The Lotus Valve incorporates an innovative Adaptive Seal designed to minimize PVL. This analysis evaluated the incidence and predictors of PVL after implantation of the Lotus transcatheter aortic valve. The REPRISE II (REpositionable Percutaneous Replacement of Stenotic Aortic Valve through Implantation of Lotus Valve System - Evaluation of Safety and Performance) Study With Extended Cohort enrolled 250 high-surgical risk patients with severe symptomatic aortic stenosis. Aortic regurgitation was assessed by echocardiography pre-procedure, at discharge and 30 days, by an independent core laboratory. Baseline and procedural predictors of mild or greater PVL at 30 days (or at discharge if 30-day data were not available) were determined using a multivariate regression model (n = 229). Of the 229 patients, 197 (86%) had no/trace PVL, 30 had mild, and 2 had moderate PVL; no patient had severe PVL. Significant predictors of mild/moderate PVL included device:annulus area ratio (odds ratio [OR] 0.87; 95% CI 0.83 to 0.92; p 10% annular oversizing by area were 17.5% (11 of 63), 2.9% (2 of 70), and 3.2% (2 of 63), respectively. Significant independent predictors of PVL included device:annulus area ratio and left ventricular outflow tract calcium volume. When the prosthetic valve was oversized by ≥5%, the rate of mild or greater PVL was only 3%. In conclusion, the overall rates of PVL with the Lotus Valve are low and predominantly related to device/annulus areas and calcium; these findings have implications for optimal device sizing. Copyright © 2017 Elsevier Inc. All rights reserved.

Urease inhibition potential of Di-naphthodiospyrol from Diospyros lotus roots.

Science.gov (United States)

Rauf, Abdur; Uddin, Ghias; Raza, Muslam; Patel, Seema; Bawazeer, Saud; Ben Hadda, Taibi; Jehan, Noor; Mabkhot, Yahia Nasser; Khan, Ajmal; Mubarak, Mohammad S

2017-05-01

The dimeric napthoquione 5,8,4'-trihydroxy-1'-methoxy-6, 6'-dimethyl-7,3'-binaphtyl-1,4,5',8'-tetraone (1) was isolated from the chloroform fraction of Diospyros lotus extract. Compound 1 was screened for its inhibitory effects against four enzymes: urease, phosphodiesterase-I, carbonic anhydrase-II and α-chymotrypsin, and showed selective activity against urease enzyme with an IC 50 value of 254.1 ± 3.82 μM as compared to the standard thiourea (IC 50  = 21 ± 0.11 μM). Furthermore, in silico docking study was carried out to explain the molecular mechanism of compound 1 against the target receptor.

Validation of a new 39 neutron group self-shielded library based on the nucleonics analysis of the Lotus fusion-fission hybrid test facility performed with the Monte Carlo code

International Nuclear Information System (INIS)

Pelloni, S.; Cheng, E.T.

1985-02-01

The Swiss LOTUS fusion-fission hybrid test facility was used to investigate the influence of the self-shielding of resonance cross sections on the tritium breeding and on the thorium ratios. Nucleonic analyses were performed using the discrete-ordinates transport codes ANISN and ONEDANT, the surface-flux code SURCU, and the version 3 of the MCNP code for the Li 2 CO 3 and the Li 2 O blanket designs with lead, thorium and beryllium multipliers. Except for the MCNP calculation which bases on the ENDF/B-V files, all nuclear data are generated from the ENDF/B-IV basic library. For the deterministic methods three NJOY group libraries were considered. The first, a 39 neutron group self-shielded library, was generated at EIR. The second bases on the same group structure as the first does and consists of infinitely diluted cross sections. Finally the third library was processed at LANL and consists of coupled 30+12 neutron and gamma groups; these cross sections are not self-shielded. The Monte Carlo analysis bases on a continuous and on a discrete 262 group library from the ENDF/B-V evaluation. It is shown that the results agree well within 3% between the unshielded libraries and between the different transport codes and theories. The self-shielding of resonance cross sections results in a decrease of the thorium capture rate and in an increase of the tritium breeding of about 6%. The remaining computed ratios are not affected by the self-shielding of cross sections. (Auth.)

Florescimento e produção de sementes de Lotus subbiflorus Lag. cv. El Rincón Flowering and seed production of Lotus subbiflorus Lag. cv. El Rincón

Directory of Open Access Journals (Sweden)

Priscila Silva da Costa Ferreira Gomes

2011-05-01

Full Text Available Objetivou-se com este trabalho avaliar a dinâmica do florescimento e o potencial de produção de sementes de Lotus subbiflorus Lag. cv. El Rincón. Por meio de amostragens semanais realizadas entre 13/11/2008 e 4/2/2009, estudaram-se as variáveis número de botões florais, número de inflorescências, número de flores, número de legumes totais, peso de mil sementes e produção de sementes. O delineamento experimental utilizado foi de blocos casualizados com os tratamentos arranjados como níveis de um fator experimental, constituído pelas datas após a semeadura. Dessa forma, o experimento foi realizado com dois blocos e doze unidades experimentais por bloco. A produção de sementes de L. subbiflorus no Sul do Brasil tem problemas decorrentes do florescimento contínuo, o que dificulta a colheita. A produção máxima obtida foi de 63,2 kg/ha em janeiro de 2009 (4372 graus-dia. O peso de mil sementes e o número de legumes foram as características que mais se correlacionaram com a produção de sementes. A emissão constante de botões florais contribui negativamente sobre os demais componentes da produção de sementes.The aim of this work was to evaluate the dynamic of the flowering and the Lotus subbiflorus Lag. Cv. "El Rincón"seed production potential. The variables studied through weekly samples from 11/13/2008 to 02/04/2009 were: number of flower buds, number of inflorescences, number of flowers, number of total legumes, weight of 1000 seeds and seed production. The experimental design used randomized blocks with treatments arranged as levels of an experimental factor, defined by the dates after seeding. The experiment was conducted with two blocks and twelve experimental units per block. Lotus subbiflorus seed production, in the South Brazil, presents problems caused by continuous flowering, difficulting to harvesting. The maximum production obtained was 63.2 kg/ha in January 2009 (4372 degree days. The weight of 1000 seeds and

Robust Superhydrophobic Carbon Nanotube Film with Lotus Leaf Mimetic Multiscale Hierarchical Structures.

Science.gov (United States)

Wang, Pengwei; Zhao, Tianyi; Bian, Ruixin; Wang, Guangyan; Liu, Huan

2017-12-26

Superhydrophobic carbon nanotube (CNT) films have demonstrated many fascinating performances in versatile applications, especially for those involving solid/liquid interfacial processes, because of their ability to affect the material/energy transfer at interfaces. Thus, developing superhydrophobic CNTs has attracted extensive research interests in the past decades, and it could be achieved either by surface coating of low-free energy materials or by constructing micro/nanohierarchical structures via various complicated processes. So far, developing a simple approach to fabricate stable superhydrophobic CNTs remains a challenge because the capillary force induced coalescence frequently happens when interacting with liquid. Herein, drawing inspirations from the lotus leaf, we proposed a simple one-step chemical vapor deposition approach with programmable controlled gas flow to directly fabricate a CNT film with rather stable superhydrophobicity, which can effectively prevent even small water droplets from permeating into the film. The robust superhydrophobicity was attributable to typical lotus-leaf-like micro/nanoscale hierarchical surface structures of the CNT film, where many microscale clusters composed of entangled nanotubes randomly protrude out of the under-layer aligned nanotubes. Consequently, dual-scale air pockets were trapped within each microscale CNT cluster and between, which could largely reduce the liquid/solid interface, leading to a Cassie state. Moreover, the superhydrophobicity of the CNT film showed excellent durability after long time exposure to air and even to corrosive liquids with a wide range of pH values. We envision that the approach developed is advantageous for versatile physicochemical interfacial processes, such as drag reduction, electrochemical catalysis, anti-icing, and biosensors.

Doğal florada yetişen sarıçiçekli gazal boynuzu (Lotus corniculatus L. ve dar yapraklı gazal boynuzunun (Lotus tenuis Waldst. & Kit. toprak tercihleri, komşu bitkileri ve yem değerleri

Directory of Open Access Journals (Sweden)

Ferat Uzun

2018-02-01

Full Text Available In this study, soil preferences of wild birdsfoot trefoil (Lotus corniculatus L. and narrowleaf birdsfoot trefoil (L tenuis Waldst. & Kit. species growing in natural flora of the Black Sea Region (Turkey and the plant species which they interact with, as well as their feed values were investigated. Dominant forage species that interact with L. corniculatus and L. tenuis were determined by the visual estimation method at 126 and 86 locations, respectively, and also seed and soil samples from each location were collected. L. corniculatus preferred soils having higher lime (90.9 vs. 66.4 g kg-1, P=0.003, pH (7.41 vs. 7.14, P=0.001 and containing lower organic matter (20.0 vs. 26.8 g kg-1, P=0.001 compared to L. tenuis. L. corniculatus was neighbor to 89 different species (20.2% legume, 22.5% grass and 57.3% others, whereas L. tenuis was neighbor to 61 different species (41.0% legume, 19.7% grass and 39.3% others. The difference between two species in terms of the frequencies of neighbor plant families was significant (2=10.814, P=0.004. Dominant plant species growing in interaction with these Lotus species were Medicago lupulina, Trifolium pratense, Trifolium repens, Cynodon dactylon, Lolium perenne and Plantago lanceolata. Dactylis glomerata was also neighbor with high frequency to L. corniculatus. L. tenuis had high phosphorus, metabolizable energy and relative feed value, and lower acid and neutral detergent fiber contents. As a result, in the artificial pasture establishments or the improvement of natural rangelands, the aforementioned species growing in harmony in natural environment and exhibiting positive interaction with Lotus species studied should be preferred.

Secondary Fitometabolitos that affect the nutritional value of Lotus corniculatus as forage for ruminant animals

OpenAIRE

Luz Elena Santacoloma Varón

2014-01-01

In the highlands of Bogota at 2600 masl, temperature 14°C and 1013 mm of precipitation, three plots have been furbished by modifying the physico- chemical conditions of the soil and planted the species Lotus corniculatus to evaluate content of condensed tannins, hydrolysable tannins, phenols, tannins that precipitate protein, saponins and alkaloids. The purpose was to determine the effect of soil conditions on the concentration of secondary phytobiomolecules. Analytical and instrumental techn...

Insect Analogue to the Lotus Leaf: A Planthopper Wing Membrane Incorporating a Low-Adhesion, Nonwetting, Superhydrophobic, Bactericidal, and Biocompatible Surface.

Science.gov (United States)

Watson, Gregory S; Green, David W; Cribb, Bronwen W; Brown, Christopher L; Meritt, Christopher R; Tobin, Mark J; Vongsvivut, Jitraporn; Sun, Mingxia; Liang, Ai-Ping; Watson, Jolanta A

2017-07-19

Nature has produced many intriguing and spectacular surfaces at the micro- and nanoscales. These small surface decorations act for a singular or, in most cases, a range of functions. The minute landscape found on the lotus leaf is one such example, displaying antiwetting behavior and low adhesion with foreign particulate matter. Indeed the lotus leaf has often been considered the "benchmark" for such properties. One could expect that there are animal counterparts of this self-drying and self-cleaning surface system. In this study, we show that the planthopper insect wing (Desudaba danae) exhibits a remarkable architectural similarity to the lotus leaf surface. Not only does the wing demonstrate a topographical likeness, but some surface properties are also expressed, such as nonwetting behavior and low adhering forces with contaminants. In addition, the insect-wing cuticle exhibits an antibacterial property in which Gram-negative bacteria (Porphyromonas gingivalis) are killed over many consecutive waves of attacks over 7 days. In contrast, eukaryote cell associations, upon contact with the insect membrane, lead to a formation of integrated cell sheets (e.g., among human stem cells (SHED-MSC) and human dermal fibroblasts (HDF)). The multifunctional features of the insect membrane provide a potential natural template for man-made applications in which specific control of liquid, solid, and biological contacts is desired and required. Moreover, the planthopper wing cuticle provides a "new" natural surface with which numerous interfacial properties can be explored for a range of comparative studies with both natural and man-made materials.

Seed colour affects light and temperature requirements during germination in two Lotus species (Fabaceae) of the Arabian subtropical deserts.

Science.gov (United States)

Bhatt, Arvind; Gairola, Sanjay; El-Keblawy, Ali A

2016-06-01

Heterogeneity in seeds mostly occurs due to physiological, environmental and genetic factors, and these could affect seed dormancy and germination. Therefore, the aim of our study was to assess the effect of seed colour on germination behavior. For this, both light and temperature requirements were assessed in Lotus glinoides and Lotus halophilus (Fabaceae) from the hyper-arid deserts of the United Arab Emirates. Germination was assessed in terms of both final germination level (percentage) and germination rate, as expressed by Timson’s germination velocity index. Lotus glinoides produces black and yellow-colored seeds, and L. halophilus produces green and yellow seeds. Different seed lots were germinated in both light and darkness at different temperatures. Yellow seeds of the two species attained significantly lower germination, compared to black and green seeds. There was no specific light or temperature requirements for the germination of the two coloured seeds of L. glinoides; the effect of interactions between seed colour and both light and incubation temperature, were not significant on the final germination percentage. In L. halophilus, green seeds germinated significantly more in both light and darkness at lower temperatures (15/25 °C) and in light at higher temperatures (25/35 °C), compared to yellow seeds. Yellow seeds germinated faster, compared to black at 15/25 °C in L. glinoides and compared to green seeds at 15/25 °C and 25/35 °C in L. halophilus. Seed colour variation, at least in L. halophilus, could be a survival strategy that would determine the time of germination throughout the year in the unpredictable desert environment.

Replication of butterfly wing and natural lotus leaf structures by nanoimprint on silica sol-gel films

International Nuclear Information System (INIS)

Saison, Tamar; Peroz, Christophe; Chauveau, Vanessa; Sondergard, Elin; Arribart, Herve; Berthier, Serge

2008-01-01

An original and low cost method for the fabrication of patterned surfaces bioinspired from butterfly wings and lotus leaves is presented. Silica-based sol-gel films are thermally imprinted from elastomeric molds to produce stable structures with superhydrophobicity values as high as 160 deg. water contact angle. The biomimetic surfaces are demonstrated to be tuned from superhydrophobic to superhydrophilic by annealing between 200 deg. C and 500 deg. C

Replication of butterfly wing and natural lotus leaf structures by nanoimprint on silica sol-gel films

Energy Technology Data Exchange (ETDEWEB)

Saison, Tamar; Peroz, Christophe; Chauveau, Vanessa; Sondergard, Elin; Arribart, Herve [Unite mixte CNRS/Saint Gobain Saint Gobain Recherche, BP135, 93303 Aubervilliers (France); Berthier, Serge [Institut des Nanosciences de Paris, UMR 7588, CNRS, Universite Pierre et Marie Curie-Paris 6, 140 rue Lourmel, 75015 Paris (France)], E-mail: cperoz@lbl.gov

2008-12-01

An original and low cost method for the fabrication of patterned surfaces bioinspired from butterfly wings and lotus leaves is presented. Silica-based sol-gel films are thermally imprinted from elastomeric molds to produce stable structures with superhydrophobicity values as high as 160 deg. water contact angle. The biomimetic surfaces are demonstrated to be tuned from superhydrophobic to superhydrophilic by annealing between 200 deg. C and 500 deg. C.

Diversification and specialization of β-glucosidases in the catabolism of hydroxynitrile glucosides in Lotus japonicus

DEFF Research Database (Denmark)

Lai, Daniela

that involves specific β-glucosidases. If plant tissue is disrupted, cyanogenic glucosides come into contact with these β-glucosidases and are hydrolyzed, which results in the release of hydrogen cyanide gas. The work reported in this thesis is focused on the β-glucosidases that activated hydroxynitrile...... glucosides in the model plant Lotus japonicus. The work highlights how closely related β-glucosidases have evolved distinct substrate specificities and differential expression patterns to serve distinct physiological and ecological roles....

Long-term outcome of microscopic esophagitis in chronic GERD patients treated with esomeprazole or laparoscopic antireflux surgery in the LOTUS trial

DEFF Research Database (Denmark)

Fiocca, Roberto; Mastracci, Luca; Engström, Cecilia

2010-01-01

OBJECTIVES: Gastroesophageal reflux disease (GERD)-associated changes in esophageal histology have been reported mainly after short-term medical antireflux therapy, and few individual lesions have been examined. We report detailed histological findings from the LOTUS study, at baseline and at 1...

The evolutionary appearance of non-cyanogenic hydroxynitrile glucosides in the Lotus genus is accompanied by the substrate specialization of paralogous beta-glucosidases resulting from a crucial amino acid substitution

DEFF Research Database (Denmark)

Lai, Daniela; Abou Hachem, Maher; Robson, Fran

2014-01-01

has the dominant physiological role in rhodiocyanoside degradation. Structural modelling, site-directed mutagenesis and activity assays establish that a glycine residue (G211) in the aglycone binding site of BGD2 is essential for its ability to hydrolyse the endogenous cyanogenic glucosides...... with the Lotus corniculatus clade within the Lotus genus. This suggests the evolutionary scenario that substrate specialization for rhodiocyanosides evolved from a promiscuous activity of a progenitor cyanogenic beta-glucosidase, resembling BGD2, and required no more than a single amino acid substitution....

Factors influencing fecundity in experimental crosses of water lotus (Nelumbo nucifera Gaertn. cultivars

Directory of Open Access Journals (Sweden)

Teng Nian-Jun

2012-06-01

Full Text Available Abstract Background Breeding programs for the water lotus (Nelumbo nucifera are hampered by an inability to account for variation in seed set associated with crosses between different cultivars. We studied seed set in two reciprocal crosses between lotus cultivars (‘Guili’ × ‘Aijiangnan’ and ‘Molingqiuse’ × ‘Qinhuaiyanzhi' to obtain insights into factors that govern fecundity in these experimental hybrids. Pollen viability, stigma receptivity and embryo development were compared for each hybrid and reciprocal cross. Results Pollen viability of the individual cultivars ranged from 4.1% to 20.2%, with the highest level (>11.9% for all cultivars observed from the earliest collected grains (05:00–06:00 a.m.. Stigmatic pollen germination peaked at 4 h after pollination and varied from 4.8 to 60.6 grains per stigma among the crosses. Production of normal embryos ranged from 7.6% to 58.8% at 1 d after pollination and from 0 to 25% by 11 d after pollination. Seed set in crosses (0.2–23.3% was generally lower than in open-pollinated plants (8.4–26.5%. Similar to the germination results, seed set was substantially reduced in both reciprocal crosses. Conclusions These results suggested that poor pollen fertility, low stigma receptivity, and embryo abortion were responsible for the failure of the crosses ‘Molingqiuse’ × ‘Qinhuaiyanzhi’, ‘Qinhuaiyanzhi’ × ‘Molingqiuse’, and ‘Aijiangnan’ × ‘Guili’.

Cytotoxic, Antitumor and Immunomodulatory Effects of the Water-Soluble Polysaccharides from Lotus (Nelumbo nucifera Gaertn. Seeds

Directory of Open Access Journals (Sweden)

Yafeng Zheng

2016-11-01

Full Text Available Lotus is an edible and medicinal plant, and the extracts from its different parts exhibit various bioactivities. In the present study, the hot water–soluble polysaccharides from lotus seeds (LSPS were evaluated for their cancer cell cytotoxicity, immunomodulatory and antitumor activities. LSPS showed significant inhibitory effects on the mouse gastric cancer MFC cells, human liver cancer HuH-7 cells and mouse hepatocarcinoma H22 cells. The animal studies showed that LSPS inhibited tumor growth in H22 tumor-bearing mice with the highest inhibition rate of 45.36%, which is comparable to that induced by cyclophosphamide (30 mg/kg treatment (50.79%. The concentrations of white blood cells were significantly reduced in cyclophosphamide-treated groups (p < 0.01, while LSPS showed much fewer side effects according to the hematology analysis. LSPS improved the immune response in H22 tumor-bearing mice by enhancing the spleen and thymus indexes, and increasing the levels of serum cytokines including tumor necrosis factor-α and interleukin-2. Moreover, LSPS also showed in vivo antioxidant activity by increasing superoxide dismutase activity, thus reducing the malondialdehyde level in the liver tissue. These results suggested that LSPS can be used as an antitumor and immunomodulatory agent.

Establishing and screening of a Lotus japonicus LORE1 mutagenized population

DEFF Research Database (Denmark)

Malolepszy, Anna

-throughput insertional mutagenesis of Lotus and that a large mutant population can be managed. Lack of insertional hotspots and a frequency of 2,4 new insertions per line, makes it an attractive tool to work with and gives the possibility to obtain cost-effective genome saturation (Urbanski et al., 2012). Additionally......, LORE1 can be successfully used for forward screening and discovery of genes involved in the nodulation process. Since LORE1 is mostly active in pollen, each plant is carrying independent insertions. Taking advantage of this feature and supporting the reliability of the large-scale set-up, well...

Expression of a complete soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus

DEFF Research Database (Denmark)

Stougaard, J; Petersen, T E; Marcker, K A

1987-01-01

The complete soybean leghemoglobin lbc(3) gene was transferred into the legume Lotus corniculatus using an Agrobacterium rhizogenes vector system. Organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with Rhizobium loti....... The primary transcript was processed in the same way as in soybean nodules and the resulting mRNA was translated into Lbc(3) protein. Quantitative determination of the Lbc(3) protein in nodules of transgenic plants indicated that the steady-state level of the soybean protein is comparable...

A Conceptual Framework for the Electronic Performance Support Systems within IBM Lotus Notes 6 (LN6 Example

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Servet BAYRAM

2005-10-01

Full Text Available A Conceptual Framework for the Electronic PerformanceSupport Systems within IBM Lotus Notes 6 (LN6 Example Assoc. Prof. Dr. Servet BAYRAM Computer Education & Instructional Technologies Marmara University, TURKEYsbayram@marmara.edu.tr ABSTRACT The concept of Electronic Performance Support Systems (EPSS is containing multimedia or computer based instruction components that improves human performance by providing process simplification, performance information and decision support system. EPSS has become a hot topic for organizational development, human resources, performance technology, training, and educational development professionals. A conceptual framework of EPSS is constructed under five interrelated and interdependent domains for educational implications. The domains of the framework are online collaboration, cost-effectiveness, motivation, service management, and performance empowering. IBM Lotus Notes 6 (LN6 is used as an example application tool to illustrate the power of this framework. The framework describes a set of relevant events based upon deductive analyses for improving our understanding of the EPSS and its implications on education and training. The article is also pointed out that there are some similarities between the EPSS’ and the LN6’s specific features within this conceptual framework. It can provide some guidelines and benefits to researchers, educators, and designers as well.

Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L.

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Nikolić Radomirka

2007-01-01

Full Text Available We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin, adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyladenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea. With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. .

Caracterização agronômica de genótipos de cornichão (Lotus corniculatus L. Agronomic characterization if birdsfoot trefoil genotypes (Lotus corniculatus L.

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Maria Tereza Bolzon Soster

2004-12-01

Full Text Available Este trabalho foi conduzido com o objetivo de caracterizar agronomicamente genótipos de cornichão (Lotus corniculatus L., incluindo oito populações do cv. São Gabriel, um cultivar rizomatoso (cv. ARS-2620 e um cultivar sem rizomas (cv. Trueno. Plantas individuais foram cultivadas e submetidas a sete cortes em condições de campo, durante um ano, em Passo Fundo, Rio Grande do Sul, Brasil. Os genótipos apresentaram variabilidade quanto ao comprimento de entrenós, estatura, diâmetro, comprimento de legumes, produção de forragem e valor nutritivo. Na média geral, as populações e o cv. Trueno foram cerca de 50% mais produtivos em relação ao cv. ARS-2620. Os genótipos apresentaram entre 80 e 100% de sobrevivência. O conteúdo de proteína bruta reduziu de 21,8% (estádio vegetativo para 11,2% (estádio de frutificação. A média geral de fibra detergente ácido foi de 24,2% e a de fibra detergente neutro variou entre 55,3 e 58,8%.This work was carried out to characterize agronomically birdsfoot trefoil (Lotus corniculatus L. genotypes, including eight populations derived from the cultivar São Gabriel, one rhizomatous cultivar (ARS-260 and one cultivar without rhizomes (Trueno. Individual plants were submitted to seven cuts under field conditions during one year, at Passo Fundo, Rio Grande do Sul, Brazil. The genotypes showed variability relative to internode lenght, height, diameter, legume lenght, forage production and nutritive value. On the average, the populations and the cv. Trueno were about 50% more productive than the cv. ARS-260. The genotypes presented a survival range from 80% to 100%. The crude protein content decreased from 21.8 (vegetative stage to 11.2% (fruiting stage. The overall mean for acid detergent fiber was 24.2% and the neutral detergent fiber ranged from 55.3% to 58.8%.

Identification of a R2R3-MYB gene regulating anthocyanin biosynthesis and relationships between its variation and flower color difference in lotus (Nelumbo Adans.

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Shan-Shan Sun

2016-09-01

Full Text Available The lotus (Nelumbonaceae: Nelumbo Adans. is a highly desired ornamental plant, comprising only two extant species, the sacred lotus (N. nucifera Gaerten. with red flowers and the American lotus (N. lutea Willd. with yellow flowers. Flower color is the most obvious difference of two species. To better understand the mechanism of flower color differentiation, the content of anthocyanins and the expression levels of four key structural genes (e.g., DFR, ANS, UFGT and GST were analyzed in two species. Our results revealed that anthocyanins were detected in red flowers, not yellow flowers. Expression analysis showed that no transcripts of GST gene and low expression level of three UFGT genes were detected in yellow flowers. In addition, three regulatory genes (NnMYB5, NnbHLH1 and NnTTG1 were isolated from red flowers and showed a high similarity to corresponding regulatory genes of other species. Sequence analysis of MYB5, bHLH1 and TTG1 in two species revealed striking differences in coding region and promoter region of MYB5 gene. Population analysis identified three MYB5 variants in Nelumbo: a functional allele existed in red flowers and two inactive forms existed in yellow flowers. This result revealed that there was an association between allelic variation in MYB5 gene and flower color difference. Yeast two-hybrid experiments showed that NnMYB5 interacts with NnbHLH1, NlbHLH1 and NnTTG1, and NnTTG1 also interacts with NnbHLH1 and NlbHLH1. The over-expression of NnMYB5 led to anthocyanin accumulation in immature seeds and flower stalks and up-regulation of expression of TT19 in Arabidopsis. Therefore, NnMYB5 is a transcription activator of anthocyanin synthesis. This study helps to elucidate the function of NnMYB5 and will contribute to clarify the mechanism of flower coloration and genetic engineering of flower color in lotus.

(D,T) Driven thorium hybrid blankets

International Nuclear Information System (INIS)

Al-Kusayer, T.A.; Khan, S.; Sahin, S.

1983-01-01

Recently, a project has started, with the aim to establish the neutronic performance and the basic design of an experimental fusionfission (hybrid) reactor facility, called AYMAN, in cylinderical geometry. The fusion reactor will have to be simulated by a (D,T) neutron generator. Fissile and fertile fuel will have to surround the neutron generator as a cylinderical blanket to simulate the boundary conditions of the hybrid blanket in a proper way. This geometry is consistent with Tandem Mirror Hybrid Blanket design and with most of the ICF blanket designs. A similar experimental installation will become operational around 1984 at the Swiss Federal Institute of Technology in Lausanne, Switzerland known under the project LOTUS. Due to the limited dimensions of the experimental cavity of the LOTUS-hybrid reactor, the LOTUS blankets have to be designed in plane geometry. Also, the bulky form of the Haefely neutron generator of the LOTUS facility obliges one to design a blanket in the plane geometry. This results in a vacuum left boundary conditions for the LOTUS blanket. The importance of a reflecting left boundary condition on the overall neutronic performance of a hybrid blanket has been analyzed in previous work in detail

LC/MS Guided Isolation of Alkaloids from Lotus Leaves by pH-Zone-Refining Counter-Current Chromatography

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Rui-Lin Hu

2011-03-01

Full Text Available The traditional methods used in natural product separation primarily target the major components and the minor components may thus be lost during the separation procedure. Consequently, it’s necessary to develop efficient methods for the preparative separation and purification of relatively minor bioactive components. In this paper, a LC/MS method was applied to guide the separation of crude extract of lotus (Nelumbo nucifera Gaertn. leaves whereby a minor component was identified in the LC/MS analysis. Afterwards, an optimized pH-zone-refining CCC method was performed to isolate this product, identified as N-demethylarmepavine. The separation procedure was carried out with a biphasic solvent system composed of hexane-ethyl acetate-methyl alcohol-water (1:6:1:6, v/v with triethylamine (10 mM added to the upper organic phase as a retainer and hydrochloric acid (5 mM to the aqueous mobile phase eluent. Two structurally similar compounds – nuciferine and roemerine – were also obtained from the crude lotus leaves extract. In total 500 mg of crude extract furnished 7.4 mg of N-demethylarmepavine, 45.3 mg of nuciferine and 26.6 mg of roemerine with purities of 90%, 92% and 96%, respectively. Their structures were further identified by HPLC/ESI-MSn, FTICR/MS and the comparison with reference compounds.

Characteristics of the Lotus japonicus gene repertoire deduced from large-scale expressed sequence tag (EST) analysis.

Science.gov (United States)

Asamizu, Erika; Nakamura, Yasukazu; Sato, Shusei; Tabata, Satoshi

2004-02-01

To perform a comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 74472 3'-end expressed sequence tags (EST) were generated from cDNA libraries produced from six different organs. Clustering of sequences was performed with an identity criterion of 95% for 50 bases, and a total of 20457 non-redundant sequences, 8503 contigs and 11954 singletons were generated. EST sequence coverage was analyzed by using the annotated L. japonicus genomic sequence and 1093 of the 1889 predicted protein-encoding genes (57.9%) were hit by the EST sequence(s). Gene content was compared to several plant species. Among the 8503 contigs, 471 were identified as sequences conserved only in leguminous species and these included several disease resistance-related genes. This suggested that in legumes, these genes may have evolved specifically to resist pathogen attack. The rate of gene sequence divergence was assessed by comparing similarity level and functional category based on the Gene Ontology (GO) annotation of Arabidopsis genes. This revealed that genes encoding ribosomal proteins, as well as those related to translation, photosynthesis, and cellular structure were more abundantly represented in the highly conserved class, and that genes encoding transcription factors and receptor protein kinases were abundantly represented in the less conserved class. To make the sequence information and the cDNA clones available to the research community, a Web database with useful services was created at http://www.kazusa.or.jp/en/plant/lotus/EST/.

Alkalinity of Lanzarote soils is a factor shaping rhizobial populations with Sinorhizobium meliloti being the predominant microsymbiont of Lotus lancerottensis.

Science.gov (United States)

León-Barrios, Milagros; Pérez-Yépez, Juan; Dorta, Paola; Garrido, Ana; Jiménez, Concepción

2017-04-01

Lotus lancerottensis is an endemic species that grows widely throughout Lanzarote Island (Canary Is.). Characterization of 48 strains isolated from root nodules of plants growing in soils from eleven locations on the island showed that 38 isolates (79.1%) belonged to the species Sinorhizobium meliloti, whereas only six belonged to Mesorhizobium sp., the more common microsymbionts for the Lotus. Other genotypes containing only one isolate were classified as Pararhizobium sp., Sinorhizobium sp., Phyllobacterium sp. and Bradyrhizobium-like. Strains of S. meliloti were distributed along the island and, in most of the localities they were exclusive or major microsymbionts of L. lancerottensis. Phylogeny of the nodulation nodC gene placed the S. meliloti strains within symbiovar lancerottense and the mesorhizobial strains with the symbiovar loti. Although strains from both symbiovars produced effective N 2 -fixing nodules, S. meliloti symbiovar lancerottense was clearly the predominant microsymbiont of L. lancerottensis. This fact correlated with the better adaptation of strains of this species to the alkaline soils of Lanzarote, as in vitro characterization showed that while the mesorhizobial strains were inhibited by alkaline pH, S. meliloti strains grew well at pH 9. Copyright © 2017 Elsevier GmbH. All rights reserved.

The WRKY Transcription Factor Genes in Lotus japonicus.

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Song, Hui; Wang, Pengfei; Nan, Zhibiao; Wang, Xingjun

2014-01-01

WRKY transcription factor genes play critical roles in plant growth and development, as well as stress responses. WRKY genes have been examined in various higher plants, but they have not been characterized in Lotus japonicus. The recent release of the L. japonicus whole genome sequence provides an opportunity for a genome wide analysis of WRKY genes in this species. In this study, we identified 61 WRKY genes in the L. japonicus genome. Based on the WRKY protein structure, L. japonicus WRKY (LjWRKY) genes can be classified into three groups (I-III). Investigations of gene copy number and gene clusters indicate that only one gene duplication event occurred on chromosome 4 and no clustered genes were detected on chromosomes 3 or 6. Researchers previously believed that group II and III WRKY domains were derived from the C-terminal WRKY domain of group I. Our results suggest that some WRKY genes in group II originated from the N-terminal domain of group I WRKY genes. Additional evidence to support this hypothesis was obtained by Medicago truncatula WRKY (MtWRKY) protein motif analysis. We found that LjWRKY and MtWRKY group III genes are under purifying selection, suggesting that WRKY genes will become increasingly structured and functionally conserved.

Lotus tenuis Seedling Establishment and Biomass Production in Flooding Pampa Grasslands (Buenos Aires , Argentina Establecimiento de Plántulas y Producción de Biomasa de Lotus tenuis en Pastizales de la Pampa Deprimida (Buenos Aires, Argentina

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Osvaldo R Vignolio

2011-03-01

Full Text Available Biomass and plant density of Lotus tenuis Waldst. & Kit. ex Willd. have been reported in decreasing in grasslands and pastures. Our objective was to determine if L. tenuis biomass and plant density can be increased in grassland through seed addition. Two separated experiments under cattle grazing exclusion were conducted in three paddocks of a Flooding Pampa grassland. The first experiment was from autumn 2004 to autumn 2006 and the second from autumn 2005 to autumn 2007. Different L. tenuis seed additions (0, 57, 229, 917 and 1833 seeds m-² were broadcast into experimental plots. In the second experiment, besides seed additions there was a reseeding of approximately 900 seed m-² from seed rain produced by plants of grassland. Seed density explained the 81% and 19% of the variation in seedling density and L. tenuis biomass, respectively. Seedling emergence occurred mainly between autumn and early spring, while seedling mortality was mainly between late spring and early summer. Lotus tenuis adult plant density and biomass production increased with seed additions. Total biomass production in the plant community varied between 589.94 ± 26.89 and 1042.44 ± 54.39 g m-² yr-1 and the differences were principally attributed to precipitations. Lotus tenuis biomass contribution was of approximately 10%. The results suggest that L. tenuis seedling and plant establishment and biomass production can be increased through seed addition and/or seed rain through grazing exclusion during reproductive period.En pastizales y pasturas ha sido documentada la reducción de la densidad de plantas y de la biomasa de Lotus tenuis Waldst. & Kit. ex Willd. Nuestro objetivo fue determinar si su densidad de plantas y su producción de biomasa pueden ser incrementadas en un pastizal mediante la adición de semillas. Dos experimentos sin pastoreo fueron realizados en tres potreros de un pastizal de la Pampa Deprimida. El primer experimento fue realizado entre otoño 2004

Facile Method for Fabricating Superhydrophobic Surface on Magnesium

Energy Technology Data Exchange (ETDEWEB)

Han, Mun Hee; Park, Yeon Hwa; Hyun, June Won; Ahn, Yong Hyun [Dankook Univ., Yongin (Korea, Republic of)

2010-04-15

In conclusion, we have developed a simple and inexpensive method for fabricating a superhydrophobic surface of magnesium by metal deposition and stearic acid coating. We fabricated a superhydrophobic surface on magnesium by nickel deposition and surface coating of stearic acid. The fabricated surfaces were stable against acidic and basic solutions. In recent times, technologies based on the imitation of nature have attracted considerable attention. Lotus leaves are known for their self-cleaning effect. The micrometer-scale papillae structure and the epicuticular wax on the lotus leaf contribute to this effect. In a manner similar to the self-cleaning property of lotus leaves, the wettability of solid surfaces is of great interest in daily life and industry.1-4 Wettability is controlled by both the geometrical structure of a surface and a low surface energy material coating. A superhydrophobic surface is satisfied with a water contact angle of more than 150 .deg. and a sliding angle of less than 10 .deg. On such a surface, a water drop has a perfectly spherical shape and it easily rolls off and removes deposited contaminants. A superhydrophobic surface thus protects a material from contamination, fogging, and snow deposition.

Differential conserted activity induced regulation of Nogo receptors (1-3, LOTUS and Nogo mRNA in mouse brain.

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Tobias E Karlsson

Full Text Available Nogo Receptor 1 (NgR1 mRNA is downregulated in hippocampal and cortical regions by increased neuronal activity such as a kainic acid challenge or by exposing rats to running wheels. Plastic changes in cerebral cortex in response to loss of specific sensory inputs caused by spinal cord injury are also associated with downregulation of NgR1 mRNA. Here we investigate the possible regulation by neuronal activity of the homologous receptors NgR2 and NgR3 as well as the endogenous NgR1 antagonist LOTUS and the ligand Nogo. The investigated genes respond to kainic acid by gene-specific, concerted alterations of transcript levels, suggesting a role in the regulation of synaptic plasticity, Downregulation of NgR1, coupled to upregulation of the NgR1 antagonist LOTUS, paired with upregulation of NgR2 and 3 in the dentate gyrus suggest a temporary decrease of Nogo/OMgp sensitivity while CSPG and MAG sensitivity could remain. It is suggested that these activity-synchronized temporary alterations may serve to allow structural alterations at the level of local synaptic circuitry in gray matter, while maintaining white matter pathways and that subsequent upregulation of Nogo-A and NgR1 transcript levels signals the end of such a temporarily opened window of plasticity.

How planting configuration influences plant secondary metabolites and total N in tall fescue (Festuca arundinacea Schreb.), alfalfa (Medicago sativa L.) and birdsfoot trefoil (Lotus corniculatus L.)

Science.gov (United States)

Theories suggest that incorporating alfalfa (Medicago sativa L.; Alf) or birdsfoot trefoil (Lotus corniculatus L.; BFT) into endophyte-infected tall fescue (Festuca arundinaceas Schreb.; E+TF) pasturelands may improve livestock production. We investigated how planting configuration might influence p...

Tales of Recombinant Femininity: "The Reincarnation of Golden Lotus," the "Chin P'ing Mei," and the Politics of Melodrama in Hong Kong.

Science.gov (United States)

Fore, Steve

1993-01-01

Examines the film "The Reincarnation of Golden Lotus" and the text upon which it is loosely based, the "Chin P'ing Mei," and the relationship between it and modern Chinese culture. States that the film's screenplay is much less an adaptation of the source novel than it is a female-centered reworking of a particular narrative…

Proteome analysis of pod and seed development in the model legume Lotus japonicus

DEFF Research Database (Denmark)

Nautrup-Pedersen, G.; Dam, S.; Laursen, B. S.

2010-01-01

Legume pods serve important functions during seed development and are themselves sources of food and feed. Compared to seeds, the metabolism and development of pods are not well-defined. The present characterization of pods from the model legume Lotus japonicus, together with the detailed analyses...... of the pod and seed proteomes in five developmental stages, paves the way for comparative pathway analysis and provides new metabolic information. Proteins were analyzed by two-dimensional gel electrophoresis and tandem-mass spectrometry. These analyses lead to the identification of 604 pod proteins and 965...... and photosynthesis. Proteins detected only in pods included three enzymes participating in the urea cycle and four in nitrogen and amino group metabolism, highlighting the importance of nitrogen metabolism during pod development. Additionally, five legume seed proteins previously unassigned in the glutamate...

Two-dimensional cross-section sensitivity and uncertainty analysis of the LBM experience at LOTUS

International Nuclear Information System (INIS)

Davidson, J.W.; Dudziak, D.J.; Pelloni, S.; Stepanek, J.

1989-01-01

In recent years, the LOTUS fusion blanket facility at IGA-EPF in Lausanne provided a series of irradiation experiments with the Lithium Blanket Module (LBM). The LBM has both realistic fusion blanket and materials and configuration. It is approximately an 80-cm cube, and the breeding material is Li 2 . Using as the D-T neutron source the Haefely Neutron Generator (HNG) with an intensity of about 5·10 12 n/s, a series of experiments with the bare LBM as well as with the LBM preceded by Pb, Be and ThO 2 multipliers were carried out. In a recent common Los Alamos/PSI effort, a sensitivity and nuclear data uncertainty path for the modular code system AARE (Advanced Analysis for Reactor Engineering) was developed. This path includes the cross-section code TRAMIX, the one-dimensional finite difference S n -transport code ONEDANT, the two-dimensional finite element S n -transport code TRISM, and the one- and two-dimensional sensitivity and nuclear data uncertainty code SENSIBL. For the nucleonic transport calculations, three 187-neutron-group libraries are presently available: MATXS8A and MATXS8F based on ENDF/B-V evaluations and MAT187 based on JEF/EFF evaluations. COVFILS-2, a 74-group library of neutron cross-sections, scattering matrices and covariances, is the data source for SENSIBL; the 74-group structure of COVFILS-2 is a subset of the Los Alamos 187-group structure. Within the framework of the present work a complete set of forward and adjoint two-dimensional TRISM calculations were performed both for the bare, as well as for the Pb- and Be-preceded, LBM using MATXS8 libraries. Then a two-dimensional sensitivity and uncertainty analysis for all cases was performed

Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds

Energy Technology Data Exchange (ETDEWEB)

Luo, Shanwei [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); Zhou, Libin, E-mail: libinzhou@impcas.ac.cn [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); Li, Wenjian; Du, Yan [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); Yu, Lixia; Feng, Hui; Mu, Jinhu [Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000 (China); University of Chinese Academy of Sciences, Beijing 100049 (China); Chen, Yuze [College of Life Science and Technology, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou, Gansu Province 730070 (China)

2016-09-15

Carbon ion beam irradiation is a powerful method for creating mutants and has been used in crop breeding more and more. To investigate the effects of carbon ion beams on Lotus japonicus, dry seeds were irradiated by 80 MeV/u carbon ion beam at dosages of 0, 100, 200, 300, 400, 500 and 600 Gy. The germination rate, survival rate and root length of M{sub 1} populations were explored and the dose of 400 Gy was selected as the median lethal dose (LD{sub 50}) for a large-scale mutant screening. Among 2472 M{sub 2} plants, 127 morphological mutants including leaf, stem, flower and fruit phenotypic variation were found, and the mutation frequency was approximately 5.14%. Inter simple sequence repeat (ISSR) assays were utilized to investigate the DNA polymorphism between seven mutants and eight plants without phenotypic variation from M{sub 2} populations. No remarkable differences were detected between these two groups, and the total polymorphic rate was 0.567%.

Novel SINEs families in Medicago truncatula and Lotus japonicus: bioinformatic analysis.

Science.gov (United States)

Gadzalski, Marek; Sakowicz, Tomasz

2011-07-01

Although short interspersed elements (SINEs) were discovered nearly 30 years ago, the studies of these genomic repeats were mostly limited to animal genomes. Very little is known about SINEs in legumes--one of the most important plant families. Here we report identification, genomic distribution and molecular features of six novel SINE elements in Lotus japonicus (named LJ_SINE-1, -2, -3) and Medicago truncatula (MT_SINE-1, -2, -3), model species of legume. They possess all the structural features commonly found in short interspersed elements including RNA polymerase III promoter, polyA tail and flanking repeats. SINEs described here are present in low to moderate copy numbers from 150 to 3000. Bioinformatic analyses were used to searched public databases, we have shown that three of new SINE elements from M. truncatula seem to be characteristic of Medicago and Trifolium genera. Two SINE families have been found in L. japonicus and one is present in both M. truncatula and L. japonicus. In addition, we are discussing potential activities of the described elements. Copyright © 2011 Elsevier B.V. All rights reserved.

Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stress

KAUST Repository

Liu, Junwei; He, Hanzi; Vitali, Marco; Visentin, Ivan; Charnikhova, Tatsiana V.; Haider, Imran; Schubert, Andrea; Ruyter-Spira, Carolien P.; Bouwmeester, Harro J J; Lovisolo, Claudio; Cardinale, Francesca

2015-01-01

Main conclusion: Strigolactone changes and cross talk with ABA unveil a picture of root-specific hormonal dynamics under stress.Abstract: Strigolactones (SLs) are carotenoid-derived hormones influencing diverse aspects of development and communication with (micro)organisms, and proposed as mediators of environmental stimuli in resource allocation processes; to contribute to adaptive adjustments, therefore, their pathway must be responsive to environmental cues. To investigate the relationship between SLs and abiotic stress in Lotus japonicus, we compared wild-type and SL-depleted plants, and studied SL metabolism in roots stressed osmotically and/or phosphate starved. SL-depleted plants showed increased stomatal conductance, both under normal and stress conditions, and impaired resistance to drought associated with slower stomatal closure in response to abscisic acid (ABA). This confirms that SLs contribute to drought resistance in species other than Arabidopsis. However, we also observed that osmotic stress rapidly and strongly decreased SL concentration in tissues and exudates of wild-type Lotus roots, by acting on the transcription of biosynthetic and transporter-encoding genes and independently of phosphate abundance. Pre-treatment with exogenous SLs inhibited the osmotic stress-induced ABA increase in wild-type roots and down-regulated the transcription of the ABA biosynthetic gene LjNCED2. We propose that a transcriptionally regulated, early SL decrease under osmotic stress is needed (but not sufficient) to allow the physiological increase of ABA in roots. This work shows that SL metabolism and effects on ABA are seemingly opposite in roots and shoots under stress.

Osmotic stress represses strigolactone biosynthesis in Lotus japonicus roots: exploring the interaction between strigolactones and ABA under abiotic stress

KAUST Repository

Liu, Junwei

2015-02-26

Main conclusion: Strigolactone changes and cross talk with ABA unveil a picture of root-specific hormonal dynamics under stress.Abstract: Strigolactones (SLs) are carotenoid-derived hormones influencing diverse aspects of development and communication with (micro)organisms, and proposed as mediators of environmental stimuli in resource allocation processes; to contribute to adaptive adjustments, therefore, their pathway must be responsive to environmental cues. To investigate the relationship between SLs and abiotic stress in Lotus japonicus, we compared wild-type and SL-depleted plants, and studied SL metabolism in roots stressed osmotically and/or phosphate starved. SL-depleted plants showed increased stomatal conductance, both under normal and stress conditions, and impaired resistance to drought associated with slower stomatal closure in response to abscisic acid (ABA). This confirms that SLs contribute to drought resistance in species other than Arabidopsis. However, we also observed that osmotic stress rapidly and strongly decreased SL concentration in tissues and exudates of wild-type Lotus roots, by acting on the transcription of biosynthetic and transporter-encoding genes and independently of phosphate abundance. Pre-treatment with exogenous SLs inhibited the osmotic stress-induced ABA increase in wild-type roots and down-regulated the transcription of the ABA biosynthetic gene LjNCED2. We propose that a transcriptionally regulated, early SL decrease under osmotic stress is needed (but not sufficient) to allow the physiological increase of ABA in roots. This work shows that SL metabolism and effects on ABA are seemingly opposite in roots and shoots under stress.

Isolation and characterization of Lotus japonicus genes involved in iron and zinc homeostasis

DEFF Research Database (Denmark)

Cvitanich, Cristina; Jensen, Winnie; Sandal, Niels Nørgaard

. Legumes are frequently grown in soil with limited nutrient availability. Plants use finely tuned mechanisms to keep appropriated levels of iron and zinc in each of their organs. Several genes involved in iron and zinc homeostasis have been described in yeast, and a few orthologs have been studied...... in plants. We have used these sequences to search for L. japonicus ESTs and genomic loci that are likely to be involved in iron and zinc metabolism. We have identified sequences corresponding to ferritins, ferric reductases, metal transport proteins of the ZIP family, and cation transporters of the NRAMP......The goal of this project is to find ways to improve the nutritional value of legumes by identifying genes and proteins important for iron and zinc regulation in the model legume Lotus japonicus. Legumes are important staples in the developing world and are a major source of nutrients in many areas...

Effect of Acacia Gum, NaCl, and Sucrose on Physical Properties of Lotus Stem Starch

Science.gov (United States)

Gill, Balmeet Singh

2014-01-01

Consumer preferences in east Asian part of the world pave the way for consumption of lotus stem starch (LSS) in preparations such as breakfast meals, fast foods, and traditional confectioneries. The present study envisaged the investigation and optimization of additives, that is, acacia gum, sodium chloride (NaCl), and sucrose, on water absorption (WA), water absorption index (WAI), and water solubility index (WSI) of LSS employing response surface methodology (RSM). Acacia gum resulted in increased water uptake and swelling of starch; however, NaCl reduced the swelling power of starch by making water unavailable to starch and also due to starch-ion electrostatic interaction. Sucrose restricted the water absorption by binding free water and decreased amylose leaching by building bridges with starch chains and thus forming rigid structure. PMID:26904639

Isolation and Characteristics of Cellulose and Nanocellulose from Lotus Leaf Stalk Agro-wastes

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Yandan Chen

2014-12-01

Full Text Available Valorization of lotus leaf stalks (LLS produced as an abundantly available agro-waste was achieved through the extraction of value-added nanocellulose. Nanofibrillated cellulose (NFC was successfully prepared from LLS by using chemical pretreatment combined with high-intensity ultrasonication. The morphological characteristics of the chemically purified LLS cellulose microfibrils were characterized by optical microscopy and MorFi fiber analysis. Fourier transform infrared (FTIR spectroscopy indicated the extensive removal of non-cellulosic components after chemical pretreatment. The transmission electron microscopy (TEM results revealed agglomeration of the developed individual NFC, with a width of 20 ± 5 nm and length on a micron scale, into a network-like feature. X-ray diffraction results showed that the resulting NFC had a cellulose I crystal structure with a high crystallinity (70%. The NFC started to degrade at around 217 °C, and the peak rate of degradation occurred at 344 °C. Nanofibrils obtained from LLS have great potential as reinforcement agents in nanocomposites.

Low permanent pacemaker rates following Lotus device implantation for transcatheter aortic valve replacement due to modified implantation protocol.

Science.gov (United States)

Krackhardt, Florian; Kherad, Behrouz; Krisper, Maximilian; Pieske, Burkert; Laule, Michael; Tschöpe, Carsten

2017-01-01

Conduction disturbances requiring permanent pacemaker implantation following transcatheter aortic valve replacement (TAVR) are a common problem. Pacemaker implantation rates after TAVR appear to be higher compared to conventional aortic valve replacement. The aim of this study was to analyze whether a high annulus implantation conveys the benefit of a decreased rate of permanent pacemaker implantation while being safe and successful according to Valve Academic Research Consortium 2 (VARC2)-criteria. A total of 23 patients with symptomatic severe aortic valve stenosis, an aortic annulus of 19-27 mm and at high risk for surgery were treated with the Lotus valve. In all patients the valve was implanted in a high annulus position via femoral access. The primary device performance endpoint was VARC2-defined device success after 30 days and the primary safety endpoint was the need for permanent pacemaker implantation. The mean age was 73.23 ± 7.65 years, 46% were female, 38% were New York Heart Association class III/IV at baseline. Thirty-day follow-up data were available for all patients. The VARC2-defined device success rate after 30 days was 22/23 (96%). 2/21 (10%) patients required a newly implanted pacemaker due to 3rd degree atrioventricular block. 25% of the patients developed a new left bundle branch block after valvuloplasty or device implantation. 21 of the 23 patients (96%) had no other signs of conduction disturbances after 30 days. The approach of the modified implantation technique of Lotus TAVR device was safe and effective. The incidence of need for a permanent pacemaker following TAVR could be significantly reduced due to adopted implantation protocol.

Phenolic Profiles and Antioxidant Activity of Lotus Root Varieties

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Yang Yi

2016-06-01

Full Text Available Lotus root attracts increasing attention mainly because of its phenolic compounds known as natural antioxidants. Its thirteen varieties were systematically analyzed on the content, distribution, composition and antioxidant activity of phenolic compounds for a better understanding of this aquatic vegetable. The respective mean contents of total phenolics in their flesh, peel and nodes were 1.81, 4.30 and 7.35 mg gallic acid equivalents (GAE/g fresh weight (FW, and those of total flavonoids were 3.35, 7.69 and 15.58 mg rutin equivalents/g FW. The phenolic composition determined by a high-performance liquid chromatography method varied significantly among varieties and parts. The phenolics of flesh were mainly composed of gallocatechin and catechin; those of peel and node were mainly composed of gallocatechin, gallic acid, catechin and epicatechin. The antioxidant activities of phenolic extracts in increasing order were flesh, peel and node; their mean concentrations for 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical were 46.00, 26.43 and 21.72 µg GAE/mL, and their mean values representing ferric reducing antioxidant power were 75.91, 87.66 and 100.43 µg Trolox equivalents/100 µg GAE, respectively. “Zoumayang”, “Baheou”, “No. 5 elian” and “Guixi Fuou” were the hierarchically clustered varieties with relatively higher phenolic content and stronger antioxidant activity as compared with the others. Especially, their nodes and peels are promising sources of antioxidants for human nutrition.

Analysis of Accumulating Ability of Heavy Metals in Lotus (Nelumbo nucifera) Improved by Ion Implantation

International Nuclear Information System (INIS)

Zhang Jianhua; Wang Naiyan; Zhang Fengshou

2012-01-01

Heavy metals have seriously contaminated soil and water, and done harm to public health. Academician WANG Naiyan proposed that ion-implantation technique should be exploited for environmental bioremediation by mutating and breeding plants or microbes. By implanting N + into Taikonglian No.1, we have selected and bred two lotus cultivars, Jingguang No.1 and Jingguang No.2. The present study aims at analyzing the feasibility that irradiation can be used for remediation of soil and water from heavy metals. Compared with parent Taikonglian No.1, the uptaking and accumulating ability of heavy metals in two mutated cultivars was obviously improved. So ion implantation technique can indeed be used in bioremediation of heavy metals in soil and water, but it is hard to select and breed a cultivar which can remedy the soil and water from all the heavy metals.

Identification of symbiotically defective mutants of Lotus japonicus affected in infection thread growth

DEFF Research Database (Denmark)

Lombardo, Fabien; Heckmann, Anne Birgitte Lau; Miwa, Hiroki

2006-01-01

During the symbiotic interaction between legumes and rhizobia, the host cell plasma membrane and associated plant cell wall invaginate to form a tunnel-like infection thread, a structure in which bacteria divide to reach the plant root cortex. We isolated four Lotus japonicus mutants that make...... infection pockets in root hairs but form very few infection threads after inoculation with Mesorhizobium loti. The few infection threads that did initiate in the mutants usually did not progress further than the root hair cell. These infection-thread deficient (itd) mutants were unaffected for early...... symbiotic responses such as calcium spiking, root hair deformation, and curling, as well as for the induction of cortical cell division and the arbuscular mycorrhizal symbiosis. Complementation tests and genetic mapping indicate that itd2 is allelic to Ljsym7, whereas the itd1, itd3, and itd4 mutations...

Genotoxicity and antigenotoxicity study of aqueous and hydro-methanol extracts of Spondias mombin L., Nymphaea lotus L. and Luffa cylindrical L. using animal bioassays

OpenAIRE

Oyeyemi, Ifeoluwa Temitayo; Yekeen, Olaide Maruf; Odusina, Paul Olayinka; Ologun, Taiwo Mary; Ogbaide, Orezimena Michelle; Olaleye, Olayinka Israel; Bakare, Adekunle A.

2015-01-01

Spondias mombin (Linn), Nymphaea lotus (Linn) and Luffa cylindrica (Linn) (syn Luffa aegyptiaca Mill) are plants traditionally used as food ingredients and in the management of diseases, including cancer, in Nigeria. Despite the therapeutic potentials attributed to these plants, reports on their genotoxicity are scanty. In this study, the genotoxicity of the aqueous and hydro-methanol extract of these plants was evaluated using mouse bone marrow micronucleus and sperm morphology assays. Antig...

Lotus japonicus nodulation requires two GRAS domain regulators, one of which is functionally conserved in a non-legume

DEFF Research Database (Denmark)

Heckmann, Anne Birgitte Lau; Lombardo, Fabien; Miwa, Hiroki

2006-01-01

A new nodulation-defective mutant of Lotus japonicus does not initiate nodule cortical cell division in response to Mesorhizobium loti, but induces root hair deformation, Nod factor-induced calcium spiking, and mycorrhization. This phenotype, together with mapping data, suggested that the mutation...... could be in the ortholog of the Medicago truncatula NSP1 gene (MtNSP1). The sequence of the orthologous gene (LjNSP1) in the L. japonicus mutant (Ljnsp1-1) revealed a mutation causing a premature stop resulting in loss of the C-terminal 23 amino acids. We also sequenced the NSP2 gene from L. japonicus...

Lotus japonicus nodulation requires two GRAS-domain regulators, NSP1 and NSP2

DEFF Research Database (Denmark)

Heckmann, Anne Birgitte Lau; Lombardo, Fabien; Miwa, Hiroki

A nodulation-defective mutant of Lotus japonicus does not initiate nodule cortical cell division in response to Mesorhizobium loti, but induces root hair deformation, Nod factor-induced calcium spiking, and mycorrhization. We have shown that this mutant has a premature stop in the NSP1 (Nodulation...... Signalling Pathway 1) gene (Ljnsp1-1) resulting in loss of the C-terminal 23 amino acids (aa) and we recently identified another mutant in NSP1 (Ljnsp1-2) with a truncated protein of 341 aa. Additionally, we have sequenced and isolated a mutant in LjNSP2 (Ljnsp2-3) that has a premature stop codon showing...

Properties of lotus seed starch-glycerin monostearin complexes formed by high pressure homogenization.

Science.gov (United States)

Chen, Bingyan; Zeng, Shaoxiao; Zeng, Hongliang; Guo, Zebin; Zhang, Yi; Zheng, Baodong

2017-07-01

Starch-lipid complexes were prepared using lotus seed starch (LS) and glycerin monostearate (GMS) via a high pressure homogenization (HPH) process, and the effect of HPH on the physicochemical properties of LS-GMS complexes was investigated. The results of Fourier transform infrared spectroscopy and complex index analysis showed that LS-GMS complexes were formed at 40MPa by HPH and the complex index increased with the increase of homogenization pressure. Scanning electron microscopy displayed LS-GMS complexes present more nest-shape structure with increasing homogenization pressure. X-ray diffraction and differential scanning calorimetry results revealed that V-type crystalline polymorph was formed between LS and GMS, with higher homogenization pressure producing an increasingly stable complex. LS-GMS complex inhibited starch granules swelling, solubility and pasting development, which further reduced peak and breakdown viscosity. During storage, LS-GMS complexes prepared by 70-100MPa had higher Avrami exponent values and lower recrystallization rates compared with native starch, which suggested a lower retrogradation trendency. Copyright © 2017 Elsevier Ltd. All rights reserved.

A Facile in Situ and UV Printing Process for Bioinspired Self-Cleaning Surfaces

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Marina A. González Lazo

2016-08-01

Full Text Available A facile in situ and UV printing process was demonstrated to create self-cleaning synthetic replica of natural petals and leaves. The process relied on the spontaneous migration of a fluorinated acrylate surfactant (PFUA within a low-shrinkage acrylated hyperbranched polymer (HBP and its chemical immobilization at the polymer-air interface. Dilute concentrations of 1 wt. % PFUA saturated the polymer-air interface within 30 min, leading to a ten-fold increase of fluorine concentration at the surface compared with the initial bulk concentration and a water contact angle (WCA of 108°. A 200 ms flash of UV light was used to chemically crosslink the PFUA at the HBP surface prior to UV printing with a polydimethylsiloxane (PDMS negative template of red and yellow rose petals and lotus leaves. This flash immobilization hindered the reverse migration of PFUA within the bulk HBP upon contacting the PDMS template, and enabled to produce texturized surfaces with WCA well above 108°. The synthetic red rose petal was hydrophobic (WCA of 125° and exhibited the adhesive petal effect. It was not superhydrophobic due to insufficient concentration of fluorine at its surface, a result of the very large increase of the surface of the printed texture. The synthetic yellow rose petal was quasi-superhydrophobic (WCA of 143°, roll-off angle of 10° and its self-cleaning ability was not good also due to lack of fluorine. The synthetic lotus leaf did not accurately replicate the intricate nanotubular crystal structures of the plant. In spite of this, the fluorine concentration at the surface was high enough and the leaf was superhydrophobic (WCA of 151°, roll-off angle below 5° and also featured self-cleaning properties.

Induction of two mutants in birdsfoot trefoil (Lotus corniculatus) by x-rays and chemical mutagens

International Nuclear Information System (INIS)

Therrien, M.C.; Grant, W.F.

1982-01-01

The mutagenic effects of X-rays, ethyl methanesulfonate (EMS), 8-ethoxycaffeine (EOC), N-hydroxyurea (HU) and 2-aminopurine (2AP) on seed treatment of birdsfoot trefoil (Lotus corniculatus L. 'Mirabel') were assessed over four generations. Mutants were recovered in the M 2 , M 3 and M 4 generations from selfed lines, from crosses derived form selfed lines and from open pollination lines. Mutant plants exhibiting vestigial floret character were recovered from X-rays, EMS, EOC and HU treatments. Mutant chlorotica plants were obtained from EMS treatment only. No mutants were recovered from 2AP treatment, EMS, the most effective mutagen, produced nine vestigial floret and 12 chlorotica mutants. Mutants were obtained from only one exposure of X-rays (12 krad). There was evidence for preferential elimination of gametes. The chlorotica and vestigial floret mutants were inherited as tetrasomic recessives. Mutation frequencies of 0.4 - 3.1% in a tetrasomic background are indicative of the effectiveness of EMS in birdsfoot trefoil

Induction of two mutants in birdsfoot trefoil (Lotus corniculatus) by x-rays and chemical mutagens

Energy Technology Data Exchange (ETDEWEB)

Therrien, M.C.; Grant, W.F. (McGill Univ., Ste. Anne de Bellevue, Quebec (Canada). Macdonald Coll.)

1982-10-01

The mutagenic effects of X-rays, ethyl methanesulfonate (EMS), 8-ethoxycaffeine (EOC), N-hydroxyurea (HU) and 2-aminopurine (2AP) on seed treatment of birdsfoot trefoil (Lotus corniculatus L. 'Mirabel') were assessed over four generations. Mutants were recovered in the M/sub 2/, M/sub 3/ and M/sub 4/ generations from selfed lines, from crosses derived form selfed lines and from open pollination lines. Mutant plants exhibiting vestigial floret character were recovered from X-rays, EMS, EOC and HU treatments. Mutant chlorotica plants were obtained from EMS treatment only. No mutants were recovered from 2AP treatment, EMS, the most effective mutagen, produced nine vestigial floret and 12 chlorotica mutants. Mutants were obtained from only one exposure of X-rays (12 krad). There was evidence for preferential elimination of gametes. The chlorotica and vestigial floret mutants were inherited as tetrasomic recessives. Mutation frequencies of 0.4 - 3.1% in a tetrasomic background are indicative of the effectiveness of EMS in birdsfoot trefoil.

Discriminating the Geographical Origins of Chinese White Lotus Seeds by Near-Infrared Spectroscopy and Chemometrics

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Lu Xu

2015-01-01

Full Text Available The traceability of a Chinese white lotus seed (WLS with Protected Designation of Origin (PDO was investigated using near-infrared (NIR spectroscopy and chemometrics. Three chemometrics methods, discrimination analysis (DA, class modeling, and a newly proposed strategy, the fusion of DA and class modeling, were investigated to compare their capacity to trace the geographical origins of WLS. Least squares support vector machine (LS-SVM was developed to distinguish the PDO WLS from non-PDO WLS of four main producing areas. A class modeling technique, one-class partial least squares (OCPLS, was developed only using the data of PDO WLS. By the fusion of LS-SVM and OCPLS, the best prediction sensitivity and specificity were 0.900 and 0.973, respectively. The results indicate that fusion of DA and class modeling can enhance the specificity for detection of non-PDO products. The conclusion is that DA and class modeling should be combined for tracing food geographical origins.

Rapid analysis of adulterations in Chinese lotus root powder (LRP) by near-infrared (NIR) spectroscopy coupled with chemometric class modeling techniques.

Science.gov (United States)

Xu, Lu; Shi, Peng-Tao; Ye, Zi-Hong; Yan, Si-Min; Yu, Xiao-Ping

2013-12-01

This paper develops a rapid analysis method for adulteration identification of a popular traditional Chinese food, lotus root powder (LRP), by near-infrared spectroscopy and chemometrics. 85 pure LRP samples were collected from 7 main lotus producing areas of China to include most if not all of the significant variations likely to be encountered in unknown authentic materials. To evaluate the model specificity, 80 adulterated LRP samples prepared by blending pure LRP with different levels of four cheaper and commonly used starches were measured and predicted. For multivariate quality models, two class modeling methods, the traditional soft independent modeling of class analogy (SIMCA) and a recently proposed partial least squares class model (PLSCM) were used. Different data preprocessing techniques, including smoothing, taking derivative and standard normal variate (SNV) transformation were used to improve the classification performance. The results indicate that smoothing, taking second-order derivatives and SNV can improve the class models by enhancing signal-to-noise ratio, reducing baseline and background shifts. The most accurate and stable models were obtained with SNV spectra for both SIMCA (sensitivity 0.909 and specificity 0.938) and PLSCM (sensitivity 0.909 and specificity 0.925). Moreover, both SIMCA and PLSCM could detect LRP samples mixed with 5% (w/w) or more other cheaper starches, including cassava, sweet potato, potato and maize starches. Although it is difficult to perform an exhaustive collection of all pure LRP samples and possible adulterations, NIR spectrometry combined with class modeling techniques provides a reliable and effective method to detect most of the current LRP adulterations in Chinese market. Copyright © 2013 Elsevier Ltd. All rights reserved.

Analysis of flavonoids from lotus (Nelumbo nucifera) leaves using high performance liquid chromatography/photodiode array detector tandem electrospray ionization mass spectrometry and an extraction method optimized by orthogonal design.

Science.gov (United States)

Chen, Sha; Wu, Ben-Hong; Fang, Jin-Bao; Liu, Yan-Ling; Zhang, Hao-Hao; Fang, Lin-Chuan; Guan, Le; Li, Shao-Hua

2012-03-02

The extraction protocol of flavonoids from lotus (Nelumbo nucifera) leaves was optimized through an orthogonal design. The solvent was the most important factor comparing solvent, solvent:tissue ratio, extraction time, and temperature. The highest yield of flavonoids was achieved with 70% methanol-water and a solvent:tissue ratio of 30:1 at 4 °C for 36 h. The optimized analytical method for HPLC was a multi-step gradient elution using 0.5% formic acid (A) and CH₃CN containing 0.1% formic acid (B), at a flow rate of 0.6 mL/min. Using this optimized method, thirteen flavonoids were simultaneously separated and identified by high performance liquid chromatography coupled with photodiode array detection/electrospray ionization mass spectrometry (HPLC/DAD/ESI-MS(n)). Five of the bioactive compounds are reported in lotus leaves for the first time. The flavonoid content of the leaves of three representative cultivars was assessed under the optimized extraction and HPLC analytical conditions, and the seed-producing cultivar 'Baijianlian' had the highest flavonoid content compared with rhizome-producing 'Zhimahuoulian' and wild floral cultivar 'Honglian'. Copyright © 2012 Elsevier B.V. All rights reserved.

Orchestrated regulation of Nogo receptors, LOTUS, AMPA receptors and BDNF in an ECT model suggests opening and closure of a window of synaptic plasticity.

Directory of Open Access Journals (Sweden)

Max Nordgren

Full Text Available Electroconvulsive therapy (ECT is an efficient and relatively fast acting treatment for depression. However, one severe side effect of the treatment is retrograde amnesia, which in certain cases can be long-term. The mechanisms behind the antidepressant effect and the amnesia are not well understood. We hypothesized that ECT causes transient downregulation of key molecules needed to stabilize synaptic structure and to prevent Ca2+ influx, and a simultaneous increase in neurotrophic factors, thus providing a short time window of increased structural synaptic plasticity. Here we followed regulation of NgR1, NgR3, LOTUS, BDNF, and AMPA subunits GluR1 and GluR2 flip and flop mRNA levels in hippocampus at 2, 4, 12, 24, and 72 hours after a single episode of induced electroconvulsive seizures (ECS in rats. NgR1 and LOTUS mRNA levels were transiently downregulated in the dentate gyrus 2, 4, 12 and 4, 12, 24 h after ECS treatment, respectively. GluR2 flip, flop and GluR1 flop were downregulated at 4 h. GluR2 flip remained downregulated at 12 h. In contrast, BDNF, NgR3 and GluR1 flip mRNA levels were upregulated. Thus, ECS treatment induces a transient regulation of factors important for neuronal plasticity. Our data provide correlations between ECS treatment and molecular events compatible with the hypothesis that both effects and side effects of ECT may be caused by structural synaptic rearrangements.

Molecular responses of Lotus japonicus to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica.

Science.gov (United States)

Hiraoka, Yukihiro; Ueda, Hiroaki; Sugimoto, Yukihiro

2009-01-01

Lotus japonicus genes responsive to parasitism by the compatible species Orobanche aegyptiaca and the incompatible species Striga hermonthica were isolated by using the suppression subtractive hybridization (SSH) strategy. O. aegyptiaca and S. hermonthica parasitism specifically induced the expression of genes involved in jasmonic acid (JA) biosynthesis and phytoalexin biosynthesis, respectively. Nodulation-related genes were almost exclusively found among the Orobanche-induced genes. Temporal gene expression analyses revealed that 19 out of the 48 Orobanche-induced genes and 5 out of the 48 Striga-induced genes were up-regulated at 1 dai. Four genes, including putative trypsin protease inhibitor genes, exhibited systemic up-regulation in the host plant parasitized by O. aegyptiaca. On the other hand, S. hermonthica attachment did not induce systemic gene expression.

A NIN-LIKE PROTEIN mediates nitrate-induced control of root nodule symbiosis in Lotus japonicus.

Science.gov (United States)

Nishida, Hanna; Tanaka, Sachiko; Handa, Yoshihiro; Ito, Momoyo; Sakamoto, Yuki; Matsunaga, Sachihiro; Betsuyaku, Shigeyuki; Miura, Kenji; Soyano, Takashi; Kawaguchi, Masayoshi; Suzaki, Takuya

2018-02-05

Legumes and rhizobia establish symbiosis in root nodules. To balance the gains and costs associated with the symbiosis, plants have developed two strategies for adapting to nitrogen availability in the soil: plants can regulate nodule number and/or stop the development or function of nodules. Although the former is accounted for by autoregulation of nodulation, a form of systemic long-range signaling, the latter strategy remains largely enigmatic. Here, we show that the Lotus japonicus NITRATE UNRESPONSIVE SYMBIOSIS 1 (NRSYM1) gene encoding a NIN-LIKE PROTEIN transcription factor acts as a key regulator in the nitrate-induced pleiotropic control of root nodule symbiosis. NRSYM1 accumulates in the nucleus in response to nitrate and directly regulates the production of CLE-RS2, a root-derived mobile peptide that acts as a negative regulator of nodule number. Our data provide the genetic basis for how plants respond to the nitrogen environment and control symbiosis to achieve proper plant growth.

Slowly digestible properties of lotus seed starch-glycerine monostearin complexes formed by high pressure homogenization.

Science.gov (United States)

Chen, Bingyan; Jia, Xiangze; Miao, Song; Zeng, Shaoxiao; Guo, Zebin; Zhang, Yi; Zheng, Baodong

2018-06-30

Starch-lipid complexes were prepared using lotus seed starch (LS) and glycerin monostearate (GMS) via a high-pressure homogenization process, and the effect of high pressure homogenization (HPH) on the slow digestion properties of LS-GMS was investigated. The digestion profiles showed HPH treatment reduced the digestive rate of LS-GMS, and the extent of this change was dependent on homogenized pressure. Scanning electron microscopy displayed HPH treatment change the morphology of LS-GMS, with high pressure producing more compact block-shape structure to resist enzyme digestion. The results of Gel-permeation chromatography and Small-angle X-ray scattering revealed high homogenization pressure impacted molecular weight distribution and semi-crystalline region of complexes, resulting in the formation of new semi-crystalline with repeat unit distance of 16-18 nm and molecular weight distribution of 2.50-2.80 × 10 5  Da, which displayed strong enzymatic resistance. Differential scanning calorimeter results revealed new semi-crystalline lamellar may originate from type-II complexes that exhibited a high transition temperature. Copyright © 2018 Elsevier Ltd. All rights reserved.

High-performance liquid chromatographic determination of β-carotene content in four varieties of lotus stamens

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Nithida Phonkot

2010-08-01

Full Text Available A high-performance liquid chromatography method was developed to determine -carotene content in lotus stamens.The stamens of four varieties of Nelumbo nucifera (Gaertn. and ten samples bought from traditional drug stores were determined.Validation of the method was carried out, the linearity of the -carotene concentrations range from 6.50-58.50 g.mL-1were 0.9997-0.9998. The repeatability and intermediate precision were 0.29 %CV (n=9 and 7.48 %CV (n=9, respectively; theaccuracy was 100.10 %w/w, the detection limit was 9.83 ng.mL-1 and the quantitation limit was 29.80 ng.mL-1. The -caroteneof the four varieties were 465.77-1150.80 mg% (n=3, and of the store samples were 4.70-41.73 mg% (n=3. The resultsdemonstrated that the contents varied according to the variety. The contents in the samples obtained from traditionaldrug stores were much lower that those dried in our laboratory, this might due to the source of the stamens, duration, andconditions of storages.

Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus (Nelumbo nucifera) using high performance liquid chromatography coupled with triple quad mass spectrometry.

Science.gov (United States)

Chen, Sha; Fang, Linchuan; Xi, Huifen; Guan, Le; Fang, Jinbao; Liu, Yanling; Wu, Benhong; Li, Shaohua

2012-04-29

Flavonoid composition and concentration were investigated in 12 different tissues of 'Ti-1' lotus (Nelumbo nucifera) by high performance liquid chromatography equipped with photodiode array detection tandem electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS(n)). A total of 20 flavonoids belonging to six groups (myricetin, quercetin, kaempferol, isohamnetin, diosmetin derivatives) were separated and identified. Myricetin 3-O-galactoside, myricetin 3-O-glucuronide, isorhamnetin 3-O-glucuronide and free aglycone diometin (3',5,7-trihydroxy-4'-methoxyflavone) were first reported in lotus. Flavonoid composition varied largely with tissue type, and diverse compounds (5-15) were found in leaf and flower stalks, flower pistils, seed coats and embryos. Flower tissues including flower petals, stamens, pistils, and, especially, reproductive tissue fruit coats had more flavonoid compounds (15-17) than leaves (12), while no flavonoids were detectable in seed kernels. The flavonoid content of seed embryos was high, 730.95 mg 100g(-1) DW (dry weight). As regards the other tissues, mature leaf pulp (771.79 mg 100 g(-1) FW (fresh weight)) and young leaves (650.67 mg 100 g(-1) FW) had higher total flavonoid amount than flower stamens (359.45 mg 100 g(-1) FW) and flower petals (342.97 mg 100g(-1) FW), while leaf stalks, flower stalks and seed coats had much less total flavonoid (less than 40 mg 100 g(-1) FW). Copyright © 2012 Elsevier B.V. All rights reserved.

Mesorhizobium bacterial strains isolated from the legume Lotus corniculatus are an alternative source for the production of polyhydroxyalkanoates (PHAs) to obtain bioplastics.

Science.gov (United States)

Marcos-García, Marta; García-Fraile, Paula; Filipová, Alena; Menéndez, Esther; Mateos, Pedro F; Velázquez, Encarna; Cajthaml, Tomáš; Rivas, Raúl

2017-07-01

Polyhydroxyalkanoic acids (PHAs) are natural polyesters that can be used to produce bioplastics which are biodegradable. Numerous microorganisms accumulate PHAs as energy reserves. Combinations of different PHAs monomers lead to the production of bioplastics with very different properties. In the present work, we show the capability of strains belonging to various phylogenetic lineages within the genus Mesorhizobium, isolated from Lotus corniculatus nodules, to produce different PHA monomers. Among our strains, we found the production of 3-hydroxybutyrate, 3-hydroxyvalerate, 3-hydroxydodecanoate, and 3-hydroxyhexadecanoate. Most of the PHA-positive strains were phylogenetically related to the species M. jarvisii. However, our findings suggest that the ability to produce different monomers forming PHAs is strain-dependent.

Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

Directory of Open Access Journals (Sweden)

Camille E Granada

2014-06-01

Full Text Available This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.

A Dicarboxylate Transporter, LjALMT4, Mainly Expressed in Nodules of Lotus japonicus.

Science.gov (United States)

Takanashi, Kojiro; Sasaki, Takayuki; Kan, Tomohiro; Saida, Yuka; Sugiyama, Akifumi; Yamamoto, Yoko; Yazaki, Kazufumi

2016-07-01

Legume plants can establish symbiosis with soil bacteria called rhizobia to obtain nitrogen as a nutrient directly from atmospheric N2 via symbiotic nitrogen fixation. Legumes and rhizobia form nodules, symbiotic organs in which fixed-nitrogen and photosynthetic products are exchanged between rhizobia and plant cells. The photosynthetic products supplied to rhizobia are thought to be dicarboxylates but little is known about the movement of dicarboxylates in the nodules. In terms of dicarboxylate transporters, an aluminum-activated malate transporter (ALMT) family is a strong candidate responsible for the membrane transport of carboxylates in nodules. Among the seven ALMT genes in the Lotus japonicus genome, only one, LjALMT4, shows a high expression in the nodules. LjALMT4 showed transport activity in a Xenopus oocyte system, with LjALMT4 mediating the efflux of dicarboxylates including malate, succinate, and fumarate, but not tricarboxylates such as citrate. LjALMT4 also mediated the influx of several inorganic anions. Organ-specific gene expression analysis showed LjALMT4 mRNA mainly in the parenchyma cells of nodule vascular bundles. These results suggest that LjALMT4 may not be involved in the direct supply of dicarboxylates to rhizobia in infected cells but is responsible for supplying malate as well as several anions necessary for symbiotic nitrogen fixation, via nodule vasculatures.

Changes in pyridine metabolism profile during growth of trigonelline-forming Lotus japonicus cell cultures.

Science.gov (United States)

Yin, Yuling; Matsui, Ayu; Sakuta, Masaaki; Ashihara, Hiroshi

2008-12-01

Changes in the profile of pyridine metabolism during growth of cells were investigated using trigonelline-forming suspension-cultured cells of Lotus japonicus. Activity of the de novo and salvage pathways of NAD biosynthesis was estimated from the in situ metabolism of [(3)H] quinolinic acid and [(14)C] nicotinamide. Maximum activity of the de novo pathway for NAD synthesis was found in the exponential growth phase, whereas activity of the salvage pathway was increased in the lag phase of cell growth. Expression profiles of some genes related to pyridine metabolism were examined using the expression sequence tags obtained from the L. japonicus database. Transcript levels of NaPRT and NIC, encoding salvage enzymes, were enhanced in the lag phase of cell growth, whereas the maximum expression of NADS was found in the exponential growth phase. Correspondingly, the activities of the salvage enzymes, nicotinate phosphoribosyltransferase (EC 2.4.2.11) and nicotinamidase (EC 3.5.1.19), increased one day after transfer of the stationary phase cells to the fresh medium. The greatest in situ trigonelline synthesis, both from [(3)H] quinolinic acid and [(14)C] nicotinamide, was found in the stationary phase of cell growth. The role of trigonelline in leguminous plants is discussed.

A Survey of Technologies Supporting Virtual Project Based Learning

DEFF Research Database (Denmark)

Dirckinck-Holmfeld, Lone

2002-01-01

This paper describes a survey of technologies and to what extent they support virtual project based learning. The paper argues that a survey of learning technologies should be related to concrete learning tasks and processes. Problem oriented project pedagogy (POPP) is discussed, and a framework...... for evaluation is proposed where negotiation of meaning, coordination and resource management are identified as the key concepts in virtual project based learning. Three e-learning systems are selected for the survey, Virtual-U, Lotus Learningspace and Lotus Quickplace, as each system offers different strategies...... for e-learning. The paper concludes that virtual project based learning may benefit from facilities of all these systems....

Genotoxicity and antigenotoxicity study of aqueous and hydro-methanol extracts of Spondias mombin L., Nymphaea lotus L. and Luffa cylindrical L. using animal bioassays.

Science.gov (United States)

Oyeyemi, Ifeoluwa Temitayo; Yekeen, Olaide Maruf; Odusina, Paul Olayinka; Ologun, Taiwo Mary; Ogbaide, Orezimena Michelle; Olaleye, Olayinka Israel; Bakare, Adekunle A

2015-12-01

Spondias mombin (Linn), Nymphaea lotus (Linn) and Luffa cylindrica (Linn) (syn Luffa aegyptiaca Mill) are plants traditionally used as food ingredients and in the management of diseases, including cancer, in Nigeria. Despite the therapeutic potentials attributed to these plants, reports on their genotoxicity are scanty. In this study, the genotoxicity of the aqueous and hydro-methanol extract of these plants was evaluated using mouse bone marrow micronucleus and sperm morphology assays. Antigenotoxicity was assessed by the bone marrow micronucleus test. The highest attainable dose of 5 000 mg/kg according to OECD guidelines was first used to assess acute toxicity of the aqueous and hydro-methanol extracts in Swiss albino mice. For each extract, there were five groups of mice (n=4/group) treated with different concentrations of the extract as against the negative and positive control group for the genotoxicity study. In the antigenotoxicity study, five groups of mice were exposed to five different concentrations of the extracts along with 60 mg/kg of methyl methane sulfonate (MMS), which was used to induce genotoxicity. The mice were administered 0.2 mL of extract per day for 10 days in the genotoxicity and antigenotoxicity groups. Administration of each of the extracts at the concentration of 5 000 mg/kg did not induce acute toxicity in mice. At the concentrations tested, all the extracts, except aqueous S. mombin, increased micronucleated polychromatic erythrocytes. The aqueous and hydro-methanol extracts of N. lotus increased the frequency of aberrant sperm cells. All the extracts were also able to ameliorate MMS induced genotoxicity in bone marrow cells of the exposed mice. The results showed the potential of the extracts to induce somatic and germ cell mutation in male mice. The extracts also ameliorated the genotoxic effect of MMS.

Genotoxicity and antigenotoxicity study of aqueous and hydro-methanol extracts of Spondias mombin L., Nymphaea lotus L. and Luffa cylindrical L. using animal bioassays

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Oyeyemi Ifeoluwa Temitayo

2015-12-01

Full Text Available Spondias mombin (Linn, Nymphaea lotus (Linn and Luffa cylindrica (Linn (syn Luffa aegyptiaca Mill are plants traditionally used as food ingredients and in the management of diseases, including cancer, in Nigeria. Despite the therapeutic potentials attributed to these plants, reports on their genotoxicity are scanty. In this study, the genotoxicity of the aqueous and hydro-methanol extract of these plants was evaluated using mouse bone marrow micronucleus and sperm morphology assays. Antigenotoxicity was assessed by the bone marrow micronucleus test. The highest attainable dose of 5 000 mg/kg according to OECD guidelines was first used to assess acute toxicity of the aqueous and hydro-methanol extracts in Swiss albino mice. For each extract, there were five groups of mice (n=4/group treated with different concentrations of the extract as against the negative and positive control group for the genotoxicity study. In the antigenotoxicity study, five groups of mice were exposed to five different concentrations of the extracts along with 60 mg/kg of methyl methane sulfonate (MMS, which was used to induce genotoxicity. The mice were administered 0.2 mL of extract per day for 10 days in the genotoxicity and antigenotoxicity groups. Administration of each of the extracts at the concentration of 5 000 mg/kg did not induce acute toxicity in mice. At the concentrations tested, all the extracts, except aqueous S. mombin, increased micronucleated polychromatic erythrocytes. The aqueous and hydro-methanol extracts of N. lotus increased the frequency of aberrant sperm cells. All the extracts were also able to ameliorate MMS induced genotoxicity in bone marrow cells of the exposed mice. The results showed the potential of the extracts to induce somatic and germ cell mutation in male mice. The extracts also ameliorated the genotoxic effect of MMS.

Selection and characterization of glyphosate tolerance in birdsfoot trefoil (Lotus corniculatus)

International Nuclear Information System (INIS)

Boerboom, C.M.

1989-01-01

If birdsfoot trefoil (Lotus corniculatus L.) was tolerant to glyphosate [N-(phosphonomethyl)glycine], Canada thistle [Cirsium arvense (L.) Scop.] and other dicot weeds could be selectively controlled in certified seed production fields. Glyphosate tolerance in birdsfoot trefoil was identified in plants from the cultivar Leo, plants regenerated from tolerant callus, and selfed progeny of plants regenerated from callus. Plants from the three sources were evaluated in field studies for tolerance to glyphosate at rates up to 1.6 kg ae/ha. Plants of Leo selected for tolerance exhibited a twofold range in the rate required to reduce shoot weight 50% (I 50 s from 0.6 to 1.2 kg/ha glyphosate). Plants regenerated from tolerant callus had tolerance up to 66% greater than plants regenerated from unselected callus. Transgressive segregation for glyphosate tolerance was observed in the selfed progeny of two regenerated plants that both had I 50 s of 0.7 kg/ha glyphosate. The selfed progeny ranged from highly tolerant (I 50 of 1.5 kg/ha) to susceptible (I 50 of 0.5 kg/ha). Spray retention, 14 C-glyphosate absorption and translocation did not account for the differential tolerance of nine plants that were evaluated from the three sources. The specific activity of 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase ranged from 1.3 to 3.5 nmol/min sm-bullet mg among the nine plants and was positively correlated with glyphosate tolerance. Leo birdsfoot trefoil was found to have significant variation in glyphosate tolerance which made it possible to initiate a recurrent selection program to select for glyphosate tolerance in birdsfoot trefoil. Two cycles of selection for glyphosate tolerance were practiced in three birdsfoot trefoil populations, Leo, Norcen, and MU-81

Seleção de populações de Lotus corniculatus L. com maior tolerância ao alumínio em solução nutritiva Selection of populations of Lotus corniculatus L. with increased tolerance to aluminum in nutrient solution

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Aline Janke

2010-11-01

Full Text Available O experimento foi realizado com o objetivo de selecionar genótipos de Lotus corniculatus L. (Draco, São Gabriel e UFRGS mais tolerantes ao alumínio, utilizando-se a técnica de seleção em solução nutritiva. A solução continha 200 µmol/L de cálcio (CaCl2, 100 µmol/L de alumínio (AlCl3 e pH controlado na faixa de 4,1 a 4,3. Realizaram-se dois ciclos de seleção, nos quais as plântulas foram selecionadas pelo comprimento final das radículas. Posteriormente, avaliou-se o ganho genético alcançado nos ciclos de seleção, tanto nas populações originais como nas melhoradas, em um experimento com solução nutritiva com quatro concentrações de alumínio (0, 50, 100 e 150 µmol/L (AlCl3, por meio do crescimento radicular líquido. O uso de solução nutritiva foi eficiente na seleção de plântulas de cornichão tolerantes ao alumínio. Os materiais analisados apresentam diferenças em relação a esta característica, destacando-se o genótipo UFRGS F2, proveniente de dois ciclos de seleção, como o material com maior tolerância ao alumínio.The experiment was carried out to select genotypes of Lotus corniculatus L. (Draco, São Gabriel and UFRGS more tolerant to aluminum, using the technique of selection in nutrient solution. The solution contained 200 µmol/L calcium (CaCl2 and 100 µmol/L aluminum (AlCl3 and a pH controlled in the range from 4.1 to 4.3. It was performed two cycles of selection, in which the seedlings were selected by the root final length. Subsequently, the genetic gain achieved in the cycles of selection was tested, in original and improved populations in an experiment in nutrient solution with four concentrations of aluminum (0, 50, 100 and 150 µmol/L (AlCl3, by liquid root growth. Use of nutritional solution was efficient in the selection of seedlings of birdsfoot trefoil tolerant to aluminum. The materials analyzed differ for this characteristic, with the genotype UFRGS F2, being the most tolerant.

Disponibilidade e valor nutritivo de forragem de leguminosas nativas (Adesmia DC. e exóticas (Lotus L. Availability and nutritive value of the wild leguminous (Adesmia DC. and exotics (Lotus L.

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Simone Meredith Scheffer-Basso

2001-06-01

Full Text Available O gênero Adesmia DC. possui 17 espécies nativas no Brasil, distribuídas nos Estados do Sul, cuja importância está vinculada a sua adaptação ao solo e clima regionais, além de ser de crescimento hibernal (temperadas. Este trabalho teve o objetivo comparar o padrão de acúmulo de matéria seca (MS e valor nutritivo de forragem de A. latifolia, A. punctata e A. tristis, tendo como padrão Lotus corniculatus (cornichão e L. uliginosus. O ensaio foi realizado em casa de vegetação durante 210 dias (4000 graus-dia. A disponibilidade de forragem (DF foi similar entre A. latifolia (276 g MS/m² e cornichão (275 g MS/m² e entre A. tristis (201g MS/m² e L. uliginosus (192 g MS/m², sendo que A. punctata apresentou a menor DF (155 g MS/m². A. latifolia caracterizou-se pela maior precocidade na DF, devido ao crescimento mais rápido em relação às demais espécies, sugerindo seu potencial para utilização durante a estação fria. Em relação às análises de qualidade, o teor de proteína bruta (PB nas folhas de A. latifolia foi de até 21,6% e a DIVMO atingiu 72,3%. Os maiores conteúdos de PB e DIVMO foram encontrados nas folhas de cornichão, 30,3 e 75,8%, respectivamente. A. tristis apresentou DIVMO muito baixa nos caules (34,9 a 44,7%, o que poderia limitar seu consumo por bovinos. Concluiu-se que, entre as espécies de Adesmia estudadas, A. latifolia detém o maior potencial forrageiro, sugerindo a continuidade de estudos com a espécie.The genus Adesmia DC. has 17 species native to Brazil, distributed in the Southern states, whose importance is linked to its adaptation to the soils and climatic conditions of the region, besides being an active winter-growing species (temperate. This work aimed to compare the patterns of dry matter (DM accumulation and nutritive value of A. latifolia, A. punctata and A. tristis, using Lotus corniculatus (birdsfoot trefoil and L. uliginosus (big trefoil, as checks. The experiment was carried out in

Iron-induced nitric oxide leads to an increase in the expression of ferritin during the senescence of Lotus japonicus nodules.

Science.gov (United States)

Chungopast, Sirinapa; Duangkhet, Mallika; Tajima, Shigeyuki; Ma, Jian Feng; Nomura, Mika

2017-01-01

Iron is an essential nutrient for legume-rhizobium symbiosis and accumulates abundantly in the nodules. However, the concentration of free iron in the cells is strictly controlled to avoid toxicity. It is known that ferritin accumulates in the cells as an iron storage protein. During nodule senescence, the expression of the ferritin gene, Ljfer1, was induced in Lotus japonicus. We investigated a signal transduction pathway leading to the increase of Ljfer1 in the nodule. The Ljfer1 promoter of L. japonicus contains a conserved Iron-Dependent Regulatory Sequence (IDRS). The expression of Ljfer1 was induced by the application of iron or sodium nitroprusside, which is a nitric oxide (NO) donor. The application of iron to the nodule increased the level of NO. These data strongly suggest that iron-induced NO leads to increased expression of Ljfer1 during the senescence of L. japonicus nodules. Copyright © 2016 Elsevier GmbH. All rights reserved.

A Novel Phenolic Compound, Chloroxynil, Improves Agrobacterium-Mediated Transient Transformation in Lotus japonicus.

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Kimura, Mitsuhiro; Cutler, Sean; Isobe, Sachiko

2015-01-01

Agrobacterium-mediated transformation is a commonly used method for plant genetic engineering. However, the limitations of Agrobacterium host-plant interactions and the complexity of plant tissue culture often make the production of transgenic plants difficult. Transformation efficiency in many legume species, including soybean and the common bean, has been reported to be quite low. To improve the transformation procedure in legumes, we screened for chemicals that increase the transformation efficiency of Lotus japonicus, a model legume species. A Chemical library was screened and chemicals that increase in transient transformation efficiency of L. japonicus accession, Miyakojima MG-20 were identified. The transient transformation efficiency was quantified by reporter activity in which an intron-containing reporter gene produces the GUS protein only when the T-DNA is expressed in the plant nuclei. We identified a phenolic compound, chloroxynil, which increased the genetic transformation of L. japonicus by Agrobacterium tumefaciens strain EHA105. Characterization of the mode of chloroxynil action indicated that it enhanced Agrobacterium-mediated transformation through the activation of the Agrobacterium vir gene expression, similar to acetosyringone, a phenolic compound known to improve Agrobacterium-mediated transformation efficiency. Transient transformation efficiency of L. japonicus with 5 μM chloroxynil was 60- and 6- fold higher than that of the control and acetosyringone treatment, respectively. In addition, transgenic L. japonicus lines were successfully generated by 5 μM chloroxynil treatment.Furthermore, we show that chloroxynil improves L. japonicus transformation by Agrobacterium strain GV3101 and rice transformation. Our results demonstrate that chloroxynil significantly improves Agrobacterium tumefaciens-mediated transformation efficiency of various agriculturally important crops.

A Rare Class of New Dimeric Naphtoquiones from Diospyros lotus have Multidrug Reversal and Antiproliferative Effects

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Dr. Abdur eRauf

2015-12-01

Full Text Available Three new dimeric naphthoquinones, 5,4′-dihydroxy-1′-methoxy-6,6′-dimethyl-7,3′-binaphthyl-1,4,5′,8′-tetraone (1, 5′,8′-dihydroxy-5-methoxy-6,6′-dimethyl-7,3′-binaphthyl-1,4,1′,4′-tetraone (2 and 8,5′,8′-trihydroxy-6,6′-dimethyl-7,3′-binaphthyl-1,4,1′,4′-tetraone (3, were isolated from the roots of Diospyros lotus. Their structures were elucidated by spectroscopic techniques, including 1D and 2D NMR, such as HSQC, HMBS, NOESY and J resolved. Compounds 1-3 were evaluated for their effects on the reversion of multidrug resistance (MDR mediated by P-glycoprotein through use of the rhodamine-123 exclusion screening test on human ABCB1 gene transfected L5178Y mouse T-cell lymphoma. Compounds 1-3 were also assessed for their antiproliferative and cytotoxic effects on L5178 and L5178Y mouse T-cell lymphoma lines. Both 1 and 2 exhibited promising antiproliferative and MDR-reversing effects in a dose dependent manner. The effects of the tested compounds on the activity of doxorubicin were observed to vary from slight antagonism to antagonism.

Phytochemical and biological study of the aerial parts of Lotus Lalambensis growing in Saudi Arabia

International Nuclear Information System (INIS)

El-Youssef, Hanan M.; Murphy, Brian T.; Amer, Masouda E.; Abdel-Kader, Maged S.; Kingston, David J.I.

2008-01-01

Phytochemical investigation of the aerial parts of Lotus lalambensis Schwenif resulted in the isolation and identification of 20 known compounds. Liquid-Liquid fractionation of the crude extract followed by chromatographic purification resulted in the isolation of lupeol, b-sitosterol, oleanolic acid, b-sitosterol glucoside and stigmasterol glucoside from petroleum ether fraction. The chloroform fraction afforded heptadecanol, kaempferol (1), kaempferol-3-O-a-L-rhamnoside (2), lotaustralin (3) epilotaaustralin (4), linamarin (5), kaempferol-3, 7-di-O-a-L-rhamnopyranoside (kaempferitin) (6) and ethyl-O-b-glucopyranoside (7). From the ethyl acetate fraction three simple rhamnosyl derivatives; butyl-O-a-L-rhamnopyranoside (8) methyl-O-a-L-rhamnopyranoside (9) and methyl-O-b-rhamnopyranoside (10) were obtained. Kaempferol-3-O-b-D-glucopyranoside-7-O-a-L-rhamnopyranoside (11), kaempferol-3-O-a- [b-D-glucopyranosyl-(1''''-2'''')-L- rhamnopyranoside]-7-O-a-L- rhamnopyranoside (12), kaempferol-3-O-b-D- rhamnopyranoside-7-O-a-[b-D-glucopyranosyl -(1'''-2'')-L- rhamnopyranoside] (13) and the myo-inositol (+) D-pinitol (14) were isolated from the butanol extract. The total extract and the different fractions were evaluated for their possible estrogenic, anti-inflammatory and anti-platelets aggregation activities. The chloroform extract showed the highest estrogenic activity, while the petroleum ether was the best in protection against inflammation induced by carrageenan. The strongest inhibition of platelet aggregations were observed with the aqueous fraction. (author)

Effects of endomycorrhizal infection, artificial herbivory, and parental cross on growth of Lotus corniculatus L.

Science.gov (United States)

Borowicz, V A; Fitter, A H

1990-03-01

We examined how combinations of parentage, fungicide application, and artificial herbivory influence growth and shoot phosphorus content in pre-reproductive Lotus corniculatus, using young offspring arising from three parental crosses, two of which had one parent in common. Soil with vesicular-arbuscular mycorrhizal (VAM) fungi was treated with either water or benomyl, an anti-VAM fungicide, and added to trays containing groups of four full siblings. There were two experiments; in the first no plants were clipped while in the second two of the four plants were clipped to simulate herbivory. In both experiments plants of the two related crosses accumulated more biomass and total shoot P than did plants of the third cross. Plants inoculated with watertreated soil had greater shoot mass and P concentration than did fungicide-treated replicates but the extent of increase in P concentration varied among crosses. In Experiment 2, clipping reduced root mass and resulted in higher shoot P concentration. In this experiment there was a significant interaction of fungicide application and clipping: both unclipped and clipped plants grew better in soil not treated with fungicide, but the increase in shoot mass, total mass, and total P was greater in unclipped plants. Significant interaction of fungicide treatment and clipping is most likely due to reduced availability of carbon to the roots of clipped plants, resulting in poorer symbiotic functioning.

Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules.

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Anna Zdyb

Full Text Available Jasmonic acid (JA, its derivatives and its precursor cis-12-oxo phytodienoic acid (OPDA form a group of phytohormones, the jasmonates, representing signal molecules involved in plant stress responses, in the defense against pathogens as well as in development. Elevated levels of JA have been shown to play a role in arbuscular mycorrhiza and in the induction of nitrogen-fixing root nodules. In this study, the gene families of two committed enzymes of the JA biosynthetic pathway, allene oxide synthase (AOS and allene oxide cyclase (AOC, were characterized in the determinate nodule-forming model legume Lotus japonicus JA levels were to be analysed in the course of nodulation. Since in all L. japonicus organs examined, JA levels increased upon mechanical disturbance and wounding, an aeroponic culture system was established to allow for a quick harvest, followed by the analysis of JA levels in whole root and shoot systems. Nodulated plants were compared with non-nodulated plants grown on nitrate or ammonium as N source, respectively, over a five week-period. JA levels turned out to be more or less stable independently of the growth conditions. However, L. japonicus nodules formed on aeroponically grown plants often showed patches of cells with reduced bacteroid density, presumably a stress symptom. Immunolocalization using a heterologous antibody showed that the vascular systems of these nodules also seemed to contain less AOC protein than those of nodules of plants grown in perlite/vermiculite. Hence, aeroponically grown L. japonicus plants are likely to be habituated to stress which could have affected JA levels.

Industry Application ECCS / LOCA Integrated Cladding/Emergency Core Cooling System Performance: Demonstration of LOTUS-Baseline Coupled Analysis of the South Texas Plant Model

Energy Technology Data Exchange (ETDEWEB)

Zhang, Hongbin [Idaho National Lab. (INL), Idaho Falls, ID (United States); Szilard, Ronaldo [Idaho National Lab. (INL), Idaho Falls, ID (United States); Epiney, Aaron [Idaho National Lab. (INL), Idaho Falls, ID (United States); Parisi, Carlo [Idaho National Lab. (INL), Idaho Falls, ID (United States); Vaghetto, Rodolfo [Texas A & M Univ., College Station, TX (United States); Vanni, Alessandro [Texas A & M Univ., College Station, TX (United States); Neptune, Kaleb [Texas A & M Univ., College Station, TX (United States)

2017-06-01

Under the auspices of the DOE LWRS Program RISMC Industry Application ECCS/LOCA, INL has engaged staff from both South Texas Project (STP) and the Texas A&M University (TAMU) to produce a generic pressurized water reactor (PWR) model including reactor core, clad/fuel design and systems thermal hydraulics based on the South Texas Project (STP) nuclear power plant, a 4-Loop Westinghouse PWR. A RISMC toolkit, named LOCA Toolkit for the U.S. (LOTUS), has been developed for use in this generic PWR plant model to assess safety margins for the proposed NRC 10 CFR 50.46c rule, Emergency Core Cooling System (ECCS) performance during LOCA. This demonstration includes coupled analysis of core design, fuel design, thermalhydraulics and systems analysis, using advanced risk analysis tools and methods to investigate a wide range of results. Within this context, a multi-physics best estimate plus uncertainty (MPBEPU) methodology framework is proposed.

Thermal-stable proteins of fruit of long-living Sacred Lotus Nelumbo nucifera Gaertn var. China Antique.

Science.gov (United States)

Shen-Miller, J; Lindner, Petra; Xie, Yongming; Villa, Sarah; Wooding, Kerry; Clarke, Steven G; Loo, Rachel R O; Loo, Joseph A

2013-09-01

Single-seeded fruit of the sacred lotus Nelumbo nucifera Gaertn var. China Antique from NE China have viability as long as ~1300 years determined by direct radiocarbon-dating, having a germination rate of 84%. The pericarp, a fruit tissue that encloses the single seeds of Nelumbo , is considered one of the major factors that contribute to fruit longevity. Proteins that are heat stable and have protective function may be equally important to seed viability. We show proteins of Nelumbo fruit that are able to withstand heating, 31% of which remained soluble in the 110°C-treated embryo-axis of a 549-yr-old fruit and 76% retained fluidity in its cotyledons. Genome of Nelumbo is published. The amino-acid sequences of 11 "thermal proteins" (soluble at 100°C) of modern Nelumbo embryo-axes and cotyledons, identified by mass spectrometry, Western blot and bioassay, are assembled and aligned with those of an archaeal-hyperthermophile Methancaldococcus jannaschii (Mj; an anaerobic methanogen having a growth optimum of 85°C) and with five mesophile angiosperms. These thermal proteins have roles in protection and repair under stress. More than half of the Nelumbo thermal proteins (55%) are present in the archaean Mj, indicating their long-term durability and history. One Nelumbo protein-repair enzyme exhibits activity at 100°C, having a higher heat-tolerance than that of Arabidopsis. A list of 30 sequenced but unassembled thermal proteins of Nelumbo is supplemented.

Cyanogenic glycosides in Lotus corniculatus : Their effect upon growth, energy budget, and nitrogen utilization of the southern armyworm, Spodoptera eridania.

Science.gov (United States)

Mark Scriber, J

1978-01-01

Two genotypes (one cyanogenic and the other acyanogenic) of birds-foot trefoil, Lotus corniculatus L., were used to study the effects of cyanogenic glycosides in leaf tissues upon a polyphagous herbivore, the southern armyworm, Spodoptera eridania Cram. (Lepidoptera). No differences were observed in consumption rate, assimilation efficiency, utilization of plant biomass, or metabolic costs in terms of expended calories between larvae fed acyanogenic or cyanogenic leaves. Similarly no differences were seen in the nitrogen or caloric utilization efficiencies, or in the nitrogen accumulation rate or growth rate of larvae on cyanogenic versus acyanogenic plants. Larval performance and growth on 20-week old plants was generally poorer than on 4 week old plants, however. This was reflected in slower growth, smaller pupal weights, lower nitrogen utilization efficiencies (N.U.E.) and biomass assimilation efficiencies (A.D.) on both the cyanogenic and acyanogenic plants.Although useful as a deterrent to some herbivores, cyanogenesis does not seem to provide an effective defense against this "adapted" herbivore. This study supports current hypotheses of insect/plant coevolution, and suggests that the metabolic costs of processing cyanogenic plant biomass are small in comparison to those imposed by the nutritional status of the plant leaves.

Nitrogen-Fixing Nodules Are an Important Source of Reduced Sulfur, Which Triggers Global Changes in Sulfur Metabolism in Lotus japonicus.

Science.gov (United States)

Kalloniati, Chrysanthi; Krompas, Panagiotis; Karalias, Georgios; Udvardi, Michael K; Rennenberg, Heinz; Herschbach, Cornelia; Flemetakis, Emmanouil

2015-09-01

We combined transcriptomic and biochemical approaches to study rhizobial and plant sulfur (S) metabolism in nitrogen (N) fixing nodules (Fix(+)) of Lotus japonicus, as well as the link of S-metabolism to symbiotic nitrogen fixation and the effect of nodules on whole-plant S-partitioning and metabolism. Our data reveal that N-fixing nodules are thiol-rich organs. Their high adenosine 5'-phosphosulfate reductase activity and strong (35)S-flux into cysteine and its metabolites, in combination with the transcriptional upregulation of several rhizobial and plant genes involved in S-assimilation, highlight the function of nodules as an important site of S-assimilation. The higher thiol content observed in nonsymbiotic organs of N-fixing plants in comparison to uninoculated plants could not be attributed to local biosynthesis, indicating that nodules are an important source of reduced S for the plant, which triggers whole-plant reprogramming of S-metabolism. Enhanced thiol biosynthesis in nodules and their impact on the whole-plant S-economy are dampened in plants nodulated by Fix(-) mutant rhizobia, which in most respects metabolically resemble uninoculated plants, indicating a strong interdependency between N-fixation and S-assimilation. © 2015 American Society of Plant Biologists. All rights reserved.

Two putative-aquaporin genes are differentially expressed during arbuscular mycorrhizal symbiosis in Lotus japonicus

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Giovannetti Marco

2012-10-01

Full Text Available Abstract Background Arbuscular mycorrhizas (AM are widespread symbioses that provide great advantages to the plant, improving its nutritional status and allowing the fungus to complete its life cycle. Nevertheless, molecular mechanisms that lead to the development of AM symbiosis are not yet fully deciphered. Here, we have focused on two putative aquaporin genes, LjNIP1 and LjXIP1, which resulted to be upregulated in a transcriptomic analysis performed on mycorrhizal roots of Lotus japonicus. Results A phylogenetic analysis has shown that the two putative aquaporins belong to different functional families: NIPs and XIPs. Transcriptomic experiments have shown the independence of their expression from their nutritional status but also a close correlation with mycorrhizal and rhizobial interaction. Further transcript quantification has revealed a good correlation between the expression of one of them, LjNIP1, and LjPT4, the phosphate transporter which is considered a marker gene for mycorrhizal functionality. By using laser microdissection, we have demonstrated that one of the two genes, LjNIP1, is expressed exclusively in arbuscule-containing cells. LjNIP1, in agreement with its putative role as an aquaporin, is capable of transferring water when expressed in yeast protoplasts. Confocal analysis have demonstrated that eGFP-LjNIP1, under its endogenous promoter, accumulates in the inner membrane system of arbusculated cells. Conclusions Overall, the results have shown different functionality and expression specificity of two mycorrhiza-inducible aquaporins in L. japonicus. One of them, LjNIP1 can be considered a novel molecular marker of mycorrhizal status at different developmental stages of the arbuscule. At the same time, LjXIP1 results to be the first XIP family aquaporin to be transcriptionally regulated during symbiosis.

COMPOSICIÓN QUIMICA DE LA LECHE, DIGESTIBILIDAD in vitro DE LA MATERIA SECA Y PRODUCCIÓN EN VACAS ALIMENTADAS CON GRAMÍNEAS SOLAS O ASOCIADAS CON Lotus uliginosus

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A. Morales

2013-01-01

Full Text Available La presente investigación tuvo como objetivo evaluar el uso de la leguminosa Lotus uliginosus como alternativa de inclusión dentro de las praderas en dos ecorregiones estratégicas de la sabana de Bogotá. en cada finca se evaluaron cuatro tratamientos: 1 kikuyo solo (P. clandestinum, 2 kikuyo asociado con trébol pata de pájaro ( P.clandes-tinum + L. uliginosus, 3 festuca alta sola (F. arundinacea y 4 Festuca alta asociada con trébol pata de pájaro (F. arundinacea + L. uliginosus. el número de bovinos varió de acuerdo a la capacidad de carga de cada finca. se utilizaron animales que se encontraban en el segundo tercio de lactancia durante un período experimental de siete días. en cada tratamiento se determinó la producción (L/día y calidad de leche (% de grasa, proteína y sólidos totales; en la pastura se evaluó la composición química (Ms, Fdn, Fda y la digestibilidad in vitro de la materia seca (divMs. se midió el consumo voluntario. en la finca Megaleche la producción de biomasa presentó diferencias significativas (P<0,05 entre los tratamientos destacándose el tratamiento del kikuyo solo; la calidad nutricional de las pasturas también presentó diferencias (P<0,04 destacándose el tratamiento de kikuyo + trébol pata de pájaro, asociación que también se destacó en consumo voluntario (P< 0.05 y producción de leche (P<0,05. en la finca Colega el contenido de proteína del forraje presentó diferencias cuando las gramíneas se asociaron con el trébol pata de pájaro (P<0,001. se concluyó que la introducción del Lotus en las praderas mejoró la producción de leche y consumo de materia seca en los animales.

A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi1

Science.gov (United States)

Guether, Mike; Neuhäuser, Benjamin; Balestrini, Raffaella; Dynowski, Marek; Ludewig, Uwe; Bonfante, Paola

2009-01-01

In mycorrhizal associations, the fungal partner assists its plant host by providing nitrogen (N) in addition to phosphate. Arbuscular mycorrhizal (AM) fungi have access to inorganic or organic forms of N and translocate them via arginine from the extra- to the intraradical mycelium, where the N is transferred to the plant without any carbon skeleton. However, the molecular form in which N is transferred, as well as the involved mechanisms, is still under debate. NH4+ seems to be the preferential transferred molecule, but no plant ammonium transporter (AMT) has been identified so far. Here, we offer evidence of a plant AMT that is involved in N uptake during mycorrhiza symbiosis. The gene LjAMT2;2, which has been shown to be the highest up-regulated gene in a transcriptomic analysis of Lotus japonicus roots upon colonization with Gigaspora margarita, has been characterized as a high-affinity AMT belonging to the AMT2 subfamily. It is exclusively expressed in the mycorrhizal roots, but not in the nodules, and transcripts have preferentially been located in the arbusculated cells. Yeast (Saccharomyces cerevisiae) mutant complementation has confirmed its functionality and revealed its dependency on acidic pH. The transport experiments using Xenopus laevis oocytes indicated that, unlike other plant AMTs, LjAMT2;2 transports NH3 instead of NH4+. Our results suggest that the transporter binds charged ammonium in the apoplastic interfacial compartment and releases the uncharged NH3 into the plant cytoplasm. The implications of such a finding are discussed in the context of AM functioning and plant phosphorus uptake. PMID:19329566

CERBERUS and NSP1 of Lotus japonicus are common symbiosis genes that modulate arbuscular mycorrhiza development.

Science.gov (United States)

Takeda, Naoya; Tsuzuki, Syusaku; Suzaki, Takuya; Parniske, Martin; Kawaguchi, Masayoshi

2013-10-01

Arbuscular mycorrhizal symbiosis (AMS) and root nodule symbiosis (RNS) are mutualistic plant-microbe interactions that confer nutritional benefits to both partners. Leguminous plants possess a common genetic system for intracellular symbiosis with AM fungi and with rhizobia. Here we show that CERBERUS and NSP1, which respectively encode an E3 ubiquitin ligase and a GRAS transcriptional regulator and which have previously only been implicated in RNS, are involved in AM fungal infection in Lotus japonicus. Hyphal elongation along the longitudinal axis of the root was reduced in the cerberus mutant, giving rise to a lower colonization level. Knockout of NSP1 decreased the frequency of plants colonized by AM fungi or rhizobia. CERBERUS and NSP1 showed different patterns of expression in response to infection with symbiotic microbes. A low constitutive level of CERBERUS expression was observed in the root and an increased level of NSP1 expression was detected in arbuscule-containing cells. Induction of AM marker gene was triggered in both cerberus and nsp1 mutants by infection with symbiotic microbes; however, the mutants showed a weaker induction of marker gene expression than the wild type, mirroring their lower level of colonization. The common symbiosis genes are believed to act in an early signaling pathway for recognition of symbionts and for triggering early symbiotic responses. Our quantitative analysis of symbiotic phenotypes revealed developmental defects of the novel common symbiosis mutants in both symbioses, which demonstrates that common symbiosis mechanisms also contribute to a range of functions at later or different stages of symbiont infection.

Fabrication and characterization of stable superhydrophobic surface with good friction-reducing performance on Al foil

Energy Technology Data Exchange (ETDEWEB)

Li, Peipei [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China); Chen, Xinhua, E-mail: xuc0374@hotmail.com [College of Chemistry and Chemical Engineering, Xuchang University, Xuchang 461000 (China); Yang, Guangbin; Yu, Laigui [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China); Zhang, Pingyu, E-mail: pingyu@henu.edu.cn [Key Laboratory of Ministry of Education for Special Functional Materials, Henan University, Kaifeng 475004 (China)

2014-05-01

Graphical abstract: A lotus-leaf-like hierarchical structure was successfully created on Al foil by a facile three-step solution–immersion method. As-obtained etched-immersed Al/STA rough surface contains interconnected convex–concave micro-structure and uniformly distributed nano-sheets that endow the surface with excellent superhydrophobicity (WCA: 164.2°; WSA: below 5°). Besides, the as-prepared etched-immersed Al/STA superhydrophobic surface on Al foil exhibits good friction-reducing ability and stable superhydrophobicity. - Highlights: • A stable superhydrophobic surface was created on aluminum foil by a facile three-step solution–immersion method. • A lotus-leaf-like hierarchical structure consists of interconnected convex–concave micro-structure and uniformly distributed nano-sheets has been constructed on the aluminum surface. • The superhydrophobic surfaces on aluminum substrate showing effective friction-reducing performance and self-cleaning ability. - Abstract: A lotus-leaf-like hierarchical structure with superhydrophobicity was created on Al foil by a facile three-step solution–immersion method involving etching in hydrochloric acid solution and immersing in hot water as well as surface-modification by stearic acid (denoted as STA). As-prepared etched-immersed Al/STA rough surface was characterized by means of scanning electron microscopy and X-ray photoelectron spectroscopy. Moreover, the water contact angles and water sliding angles of as-prepared etched-immersed Al/STA rough surface were measured, and the friction-reducing performance and self-cleaning ability of the as-prepared surface were also evaluated. Results indicate that the etched-immersed Al/STA rough surface consists of interconnected convex–concave micro-structure and uniformly distributed nano-sheets. Besides, it exhibits stable superhydrophobicity and good friction-reducing ability. Namely, it has a contact angle of water as high as 164.2° and a water sliding

Fabrication and characterization of stable superhydrophobic surface with good friction-reducing performance on Al foil

International Nuclear Information System (INIS)

Li, Peipei; Chen, Xinhua; Yang, Guangbin; Yu, Laigui; Zhang, Pingyu

2014-01-01

Graphical abstract: A lotus-leaf-like hierarchical structure was successfully created on Al foil by a facile three-step solution–immersion method. As-obtained etched-immersed Al/STA rough surface contains interconnected convex–concave micro-structure and uniformly distributed nano-sheets that endow the surface with excellent superhydrophobicity (WCA: 164.2°; WSA: below 5°). Besides, the as-prepared etched-immersed Al/STA superhydrophobic surface on Al foil exhibits good friction-reducing ability and stable superhydrophobicity. - Highlights: • A stable superhydrophobic surface was created on aluminum foil by a facile three-step solution–immersion method. • A lotus-leaf-like hierarchical structure consists of interconnected convex–concave micro-structure and uniformly distributed nano-sheets has been constructed on the aluminum surface. • The superhydrophobic surfaces on aluminum substrate showing effective friction-reducing performance and self-cleaning ability. - Abstract: A lotus-leaf-like hierarchical structure with superhydrophobicity was created on Al foil by a facile three-step solution–immersion method involving etching in hydrochloric acid solution and immersing in hot water as well as surface-modification by stearic acid (denoted as STA). As-prepared etched-immersed Al/STA rough surface was characterized by means of scanning electron microscopy and X-ray photoelectron spectroscopy. Moreover, the water contact angles and water sliding angles of as-prepared etched-immersed Al/STA rough surface were measured, and the friction-reducing performance and self-cleaning ability of the as-prepared surface were also evaluated. Results indicate that the etched-immersed Al/STA rough surface consists of interconnected convex–concave micro-structure and uniformly distributed nano-sheets. Besides, it exhibits stable superhydrophobicity and good friction-reducing ability. Namely, it has a contact angle of water as high as 164.2° and a water sliding

Common symbiosis genes CERBERUS and NSP1 provide additional insight into the establishment of arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus.

Science.gov (United States)

Nagae, Miwa; Takeda, Naoya; Kawaguchi, Masayoshi

2014-01-01

Arbuscular mycorrhizal symbiosis (AMS) and root nodule symbiosis (RNS) share several common symbiotic components, and many of the common symbiosis mutants block the entry of symbionts into the roots. We recently reported that CERBERUS (an E3 ubiquitin ligase) and NSP1 (a GRAS family transcription factor), required for RNS, also modulate AMS development in Lotus japonicus. The novel common symbiosis mutants, cerberus and nsp1, have low colonization of arbuscular mycorrhiza (AM) fungi, caused by a defect in internal hyphal elongation and by a decreased fungal entry into the roots, respectively. Here, we showed that CERBERUS was induced at the sites of symbiotic fungal or bacterial infection. NSP1 has been implicated in a strigolactone biosynthesis gene DWARF27 expression. Nevertheless, in nsp1, DWARF27 was induced by inoculation with AM fungi, implying the existence of a NSP1-independent regulatory mechanism of strigolactone biosynthesis during AMS establishment. These results support functional analysis of CERBERUS and NSP1, and also contribute to elucidation of common mechanisms in AMS and RNS.

Phyllobacterium salinisoli sp. nov., isolated from a Lotus lancerottensis root nodule in saline soil from Lanzarote.

Science.gov (United States)

León-Barrios, Milagros; Ramírez-Bahena, Martha Helena; Igual, José M; Peix, Álvaro; Velázquez, Encarna

2018-04-01

A Gram-negative rod, designated strain LLAN61 T , was isolated from a root nodule of Lotus lancerottensis growing in a saline soil sample from Lanzarote (Canary Islands). The strain grew optimally at 0.5 % (w/v) NaCl and tolerated up to 3.5 %. The 16S rRNA gene sequence analysis showed that strain LLAN61 T belonged to genus Phyllobacterium and that Phyllobacteriumleguminum ORS 1419 T and Phyllobacteriummyrsinacearum IAM 13584 T are the closest related species with 97.93 and 97.86% similarity values, respectively. In the atpD phylogeny, P. leguminum ORS 1419 T and P. myrsinacearum ATCC 43591 T , sharing similarities of 87.6 and 85.8% respectively, were also the closest species to strain LLAN61 T . DNA-DNA hybridization showed an average value of 21 % between strain LLAN61 T and P. leguminum LMG 22833 T , and 6 % with P. myrsinacearum ATCC 43590 T . The predominant fatty acids were C19 : 0 cyclo ω8c and C18 : 1ω6c/C18 : 1ω7c (summed feature 8). The DNA G+C content was 58.0 mol%. Strain LLAN61 T differed from its closest relatives in some culture conditions and in assimilation of several carbon sources. Based upon the results of phylogeny, DNA-DNA hybridization, phenotypic tests and fatty acid analysis, this strain should be classified as a novel species of Phyllobacterium for which the name Phyllobacterium salinisoli sp. nov. is proposed (type strain LLAN61 T =LMG 30173 T = CECT 9417 T ).

Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector

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Nikolić Radomirka

2007-01-01

Full Text Available Cotyledons from 6-day-old Lotus corniculatus cv. Bokor seedlings, transversally cut into two halves, were capa­ble of regenerating buds without intervening callus formation. The explants were co-cultivated with the Agrobacterium tumefaciens LBA4404/pTOK233 superbinary vector carrying the uidA-intron gene and the genes hpt and nptII. They were cultured for 14 days on a regeneration medium, then subjected to a stepwise hygromycin B selection procedure consisting of gradually increasing antibiotic concentrations (5-15 mg L-1 over 21 weeks. Transformed shoots were obtained within 5 months after co-cultivation. Out of 124 initially co-cultivated explants, 52 (42% plants survived hygromycin B selection. The presence of transgenes in regenerated plants was verified by β-glucuronidase histochemical assays and PCR analysis for the presence of uidA gene sequences. Hygromycin B-resistant and PCR-positive T0 plants were cultured in the greenhouse to produce flowers and seeds. The obtained data demonstrate that the reported transformation protocol could be useful for introducing agriculturally important genes into the new L. corniculatus cultivar Bokor.

Sample (S): SE57_S08 [Metabolonote[Archive

Lifescience Database Archive (English)

Full Text Available SE57_S08 Lotus japonicus (accessions B129 gifu and MG20 miyakojima) Lotus japonicus... NCBI taxonomy:34305 Lotus japonicus accessions were a gift from Dr. Toshio Aoki (Nihon University, Japan). ...

Genome-Wide Identification, Evolutionary Analysis and Expression Profiles of LATERAL ORGAN BOUNDARIES DOMAIN Gene Family in Lotus japonicus and Medicago truncatula.

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Tianquan Yang

Full Text Available The LATERAL ORGAN BOUNDARIES DOMAIN (LBD gene family has been well-studied in Arabidopsis and play crucial roles in the diverse growth and development processes including establishment and maintenance of boundary of developmental lateral organs. In this study we identified and characterized 38 LBD genes in Lotus japonicus (LjLBD and 57 LBD genes in Medicago truncatula (MtLBD, both of which are model legume plants that have some specific development features absent in Arabidopsis. The phylogenetic relationships, their locations in the genome, genes structure and conserved motifs were examined. The results revealed that all LjLBD and MtLBD genes could be distinctly divided into two classes: Class I and II. The evolutionary analysis showed that Type I functional divergence with some significantly site-specific shifts may be the main force for the divergence between Class I and Class II. In addition, the expression patterns of LjLBD genes uncovered the diverse functions in plant development. Interestingly, we found that two LjLBD proteins that were highly expressed during compound leaf and pulvinus development, can interact via yeast two-hybrid assays. Taken together, our findings provide an evolutionary and genetic foundation in further understanding the molecular basis of LBD gene family in general, specifically in L. japonicus and M. truncatula.

Relationships between phenotypic variation in osmotic adjustment, water-use efficiency, and drought tolerance of seven cultivars of Lotus corniculatus L.

Directory of Open Access Journals (Sweden)

Luis Inostroza

2015-03-01

Full Text Available Lotus corniculatus L. is a perennial forage legume species highly-adapted to growth under drought conditions. However, the genetic and physiological mechanisms involved in its adaptive capacity have not been elucidated. The role of osmotic adjustment (OA and water-use efficiency (WUE on the drought tolerance of L. corniculatus was studied in a greenhouse experiment. Seven cultivars of different origin were subjected to two contrasting treatments of available soil water: No water stress (NWS and with water stress (WWS. Xylem water potential (Ψx, osmotic potential (Ψπ, pressure potential (Ψp, relative water content (RWC, stomatal conductance (g s, shoot DM production, water transpiration (T, and WUE (shoot DM/T were measured. Water treatments significantly (P < 0.05 affected plant water status, which was reflected in reduced Ψx, RWC, g s, and transpiration rate in the WWS treatment compared with the NWS treatment. All cultivars showed a high capacity for OA under WWS treatment because Ψπ decreased by approximately 60% and Ψp increased by approximately 30%, compared with the NWS treatment. Cultivars with a higher solute accumulation (low Ψπ value had the lowest DM production under WWS treatment. In contrast, WUE varied greatly among cultivars and was positively associated (R² = 0.88; P < 0.01 with DM production under drought conditions.

Lotus-like effect for metal filings recovery and particle removal on heated metal surfaces using Leidenfrost water droplets.

Science.gov (United States)

Tan, Cher Lin Clara; Sapiha, Kostantyn; Leong, Yoke Fun Hannah; Choi, Siwon; Anariba, Franklin; Thio, Beng Joo Reginald

2015-07-21

A "lotus-like" effect is applied to demonstrate the ability of the Leidenfrost water droplets to recover Cu particles on a heated Al substrate. Cu particles on the heated surface adhere to the rim of the Leidenfrost droplets and eventually coat the droplets' surface to form an aggregation. When Fe filings are added to the Cu particles, the aggregated mixture can then be collected using a strong rare earth magnet (NdFeB) upon evaporation of the water. We also show that the Leidenfrost effect can be effectively utilized to recover both hydrophobic (dust and activated carbon) and hydrophilic (SiO2 and MgO) particles from heated Al surfaces without any topographical modification or surfactant addition. Our results show that hydrophobic and hydrophilic materials can be collected with >92% and >96% effectiveness on grooved and smooth Al surfaces, respectively. Furthermore, we observed no significant differences in the amount of material collected above the Leidenfrost point within the tested temperature range (240 °C vs. 340 °C) as well as when the Al sheet was replaced with a Cu sheet as the substrate. However, we did observe that the Leidenfrost droplets were able to collect a greater amount of material when the working liquid was water than when it was ethanol. Our findings show promise in the development of an effective precious coinage metal filings recovery technology for application in the mint industry, as well as the self-cleaning of metallic and semiconductor surfaces where manual cleaning is not amenable.

Dicty_cDB: Contig-U04327-1 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available 05, 5' en... 52 3e-04 2 ( BI420470 ) LjNEST57e11r Lotus japonicus nodule library ...5 and... 52 3e-04 2 ( BI417687 ) LjNEST34c3r Lotus japonicus nodule library 5 and ... 52 3e-04 2 ( BI418582 ) LjNEST43d6r Lotu...s japonicus nodule library 5 and ... 52 3e-04 2 ( BI419267 ) LjNEST44c8r Lotus japonicus nodule library... 5 and ... 52 3e-04 2 ( BI419149 ) LjNEST35h6r Lotus japonicus nodule library 5 and ... 52 3e-0... cDNA clone SOYBE... 48 0.50 1 ( BQ606814 ) BRY_2689 wheat EST endosperm library Triticu

The research on Nelumbonucifera for eutrophication control in Wuliangsuhailake Inner Mongolia,China

Science.gov (United States)

Li, Xing; Xu, Xiaoqing; Gou, Mangmang

2018-02-01

In order to study the effect on Nelumbonucifera (common name:lotus) for eutrophication control in Wuliangsuhai lake inner Mongolia, an enclosure of 2000m2was established in the southeast of Wuliangsuhailake. In the paper, the changes on water quality and phytoplankton abundance were monitored before planting lotus, after. Purpose of the study is to investigate the influence of lotus on improving water quality, restoration of water environment, and so on. The results were shown below. Firstly, Plantinglotus was significant for removal of total nitrogen and total phosphorus in water. Total nitrogen removal rate was more than 25%.Total phosphorus removal rate was more than 30%.Secondly, dissolved oxygen concentrations in August increased by 39.31percent.The Simultaneously, compared with the control (in April), the pH value was 8.0 in August(the flowering period) and declined by 10.11 percent. In this salinity, lotus had a strong salt tolerance and growed normally. Lastly, the biomass of phytoplankton was inhibited after planting lotus, especially for the inhibition of green algae growth. Studies on water bodies restoration under planting lotus in Wuliangsuhai lake have not been reported.Therefore, planting lotus will provide a new idea and theoretical reference for improving the eutrophication of Wuliangsuhailake.

Gibberellins interfere with symbiosis signaling and gene expression and alter colonization by arbuscular mycorrhizal fungi in Lotus japonicus.

Science.gov (United States)

Takeda, Naoya; Handa, Yoshihiro; Tsuzuki, Syusaku; Kojima, Mikiko; Sakakibara, Hitoshi; Kawaguchi, Masayoshi

2015-02-01

Arbuscular mycorrhiza is a mutualistic plant-fungus interaction that confers great advantages for plant growth. Arbuscular mycorrhizal (AM) fungi enter the host root and form symbiotic structures that facilitate nutrient supplies between the symbionts. The gibberellins (GAs) are phytohormones known to inhibit AM fungal infection. However, our transcriptome analysis and phytohormone quantification revealed GA accumulation in the roots of Lotus japonicus infected with AM fungi, suggesting that de novo GA synthesis plays a role in arbuscular mycorrhiza development. We found pleiotropic effects of GAs on the AM fungal infection. In particular, the morphology of AM fungal colonization was drastically altered by the status of GA signaling in the host root. Exogenous GA treatment inhibited AM hyphal entry into the host root and suppressed the expression of Reduced Arbuscular Mycorrhization1 (RAM1) and RAM2 homologs that function in hyphal entry and arbuscule formation. On the other hand, inhibition of GA biosynthesis or suppression of GA signaling also affected arbuscular mycorrhiza development in the host root. Low-GA conditions suppressed arbuscular mycorrhiza-induced subtilisin-like serine protease1 (SbtM1) expression that is required for AM fungal colonization and reduced hyphal branching in the host root. The reduced hyphal branching and SbtM1 expression caused by the inhibition of GA biosynthesis were recovered by GA treatment, supporting the theory that insufficient GA signaling causes the inhibitory effects on arbuscular mycorrhiza development. Most studies have focused on the negative role of GA signaling, whereas our study demonstrates that GA signaling also positively interacts with symbiotic responses and promotes AM colonization of the host root. © 2015 American Society of Plant Biologists. All Rights Reserved.

Second international comparison on measuring techniques of tritium production rate for fusion neutronics experiments (ICMT-2)

International Nuclear Information System (INIS)

Maekawa, Fujio; Maekawa, Hiroshi

1993-02-01

An second international comparison on measuring techniques of tritium production rates for fusion neutronics experiments (ICMT-2) has been performed. The purpose is to evaluate the measurement accuracy of tritium production rates in the current measurement techniques. Two 14 MeV neutron source facilities, FNS at JAERI-Japan and LOTUS at EPFL-Switzerland, were used for this purpose. Nine groups out of seven countries participated in this program. A fusion simulated blanket assembly of simple-geometry was served as the test bed at each facility, in which Li-containing samples from the participants were irradiated in an uniform neutron field. The tritium production rates were determined by the participants using their own ways by using the liquid scintillation counting method. Tritiated water sample with unknown but the same concentration was also distributed and its concentration was measured to make a common reference. The standard deviation of measured tritium production rates among participants was about 10 % for both FNS and LOTUS irradiation levels: 4x10 -13 T-atoms/Li-atom and 1.6x10 -12 T-atoms/Li-atom at a sample, respectively. This standard deviation exceeds the expected deviation of 5 % in this program. It is presumed that the deviation of 10 % is caused mainly by the systematic and unknown errors in a process of tritium extraction from the irradiated samples depending on each organization. (author)

Emission Facilities - Erosion & Sediment Control Facilities

Data.gov (United States)

NSGIC Education | GIS Inventory — An Erosion and Sediment Control Facility is a DEP primary facility type related to the Water Pollution Control program. The following sub-facility types related to...

Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics

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Liu Wei

2009-06-01

Full Text Available Abstract Background Transgenic approaches provide a powerful tool for gene function investigations in plants. However, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. Superroot of Lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. Recently, a technique to obtain transgenic L. corniculatus plants from Superroot-derived leaves through A. tumefaciens-mediated transformation was described. However, transformation efficiency was low and it took about six months from gene transfer to PCR identification. Results In the present study, we developed an A. rhizogenes-mediated transformation of Superroot-derived L. corniculatus for gene function investigation, combining the efficient A. rhizogenes-mediated transformation and the rapid regeneration system of Superroot. The transformation system using A. rhizogenes K599 harbouring pGFPGUSPlus was improved by validating some parameters which may influence the transformation frequency. Using stem sections with one node as explants, a 2-day pre-culture of explants, infection with K599 at OD600 = 0.6, and co-cultivation on medium (pH 5.4 at 22°C for 2 days enhanced the transformation frequency significantly. As proof of concept, Superroot-derived L. corniculatus was transformed with a gene from wheat encoding an Na+/H+ antiporter (TaNHX2 using the described system. Transgenic Superroot plants were obtained and had increased salt tolerance, as expected from the expression of TaNHX2. Conclusion A rapid and efficient tool for gene function investigation in L. corniculatus was developed, combining the simplicity and high efficiency of the Superroot regeneration system and the availability of A. rhizogenes-mediated transformation. This system was improved by validating some parameters influencing the transformation frequency, which could

Analysis of myelomonocytic leukemic differentiation by a cell surface marker panel including a fucose-binding lectin from Lotus tetragonolobus.

Science.gov (United States)

Elias, L; Van Epps, D E

1984-06-01

The fucose-binding lectin from Lotus tetragonolobus ( FBL -L) has been previously shown to bind specifically to normal cells of the myeloid and monocytic lineages. The purpose of this study was to explore the utility of fluoresceinated FBL -L as a leukemia differentiation marker in conjunction with a panel of other frequently used surface markers (Fc receptor, HLA-DR, OKM1, and antimonocyte antibody). FBL -L reacted with leukemic cells in 8/9 cases of clinically recognized acute myeloid leukemia, including myeloid blast crisis of chronic granulocytic leukemia, 3/3 cases of chronic phase chronic myelogenous leukemia, and in 2/7 cases of clinically undifferentiated acute leukemia. Correlations were noted between reactivity with FBL -L, and DR and Fc receptor expression. Among continuous cell lines, FBL -L bound with high intensity to a majority of HL-60 and U937 cells. The less well differentiated myeloblast cell lines, KG-1, KG1a , and HL-60 blast II, exhibited less FBL -L binding than HL-60 and U937. A moderate proportion of K562 cells exhibited low level binding of FBL -L. Several lymphoblastic cell lines exhibited a pattern of low intensity binding that was distinguishable from the high intensity binding pattern of the myeloblastic lines. FBL -L reactivity of U937 was enhanced by induction of differentiation with leukocyte conditioned medium, but not dimethylsulfoxide. Such treatments induced contrasting patterns of change of HL-60 and U937 when labeled with OKM1, alpha-Mono, and HLA-DR. These studies demonstrate the application of FBL -L to analysis and quantitation of myelomonocytic leukemic differentiation.

Gibberellins Interfere with Symbiosis Signaling and Gene Expression and Alter Colonization by Arbuscular Mycorrhizal Fungi in Lotus japonicus1

Science.gov (United States)

Takeda, Naoya; Handa, Yoshihiro; Tsuzuki, Syusaku; Kojima, Mikiko; Sakakibara, Hitoshi; Kawaguchi, Masayoshi

2015-01-01

Arbuscular mycorrhiza is a mutualistic plant-fungus interaction that confers great advantages for plant growth. Arbuscular mycorrhizal (AM) fungi enter the host root and form symbiotic structures that facilitate nutrient supplies between the symbionts. The gibberellins (GAs) are phytohormones known to inhibit AM fungal infection. However, our transcriptome analysis and phytohormone quantification revealed GA accumulation in the roots of Lotus japonicus infected with AM fungi, suggesting that de novo GA synthesis plays a role in arbuscular mycorrhiza development. We found pleiotropic effects of GAs on the AM fungal infection. In particular, the morphology of AM fungal colonization was drastically altered by the status of GA signaling in the host root. Exogenous GA treatment inhibited AM hyphal entry into the host root and suppressed the expression of Reduced Arbuscular Mycorrhization1 (RAM1) and RAM2 homologs that function in hyphal entry and arbuscule formation. On the other hand, inhibition of GA biosynthesis or suppression of GA signaling also affected arbuscular mycorrhiza development in the host root. Low-GA conditions suppressed arbuscular mycorrhiza-induced subtilisin-like serine protease1 (SbtM1) expression that is required for AM fungal colonization and reduced hyphal branching in the host root. The reduced hyphal branching and SbtM1 expression caused by the inhibition of GA biosynthesis were recovered by GA treatment, supporting the theory that insufficient GA signaling causes the inhibitory effects on arbuscular mycorrhiza development. Most studies have focused on the negative role of GA signaling, whereas our study demonstrates that GA signaling also positively interacts with symbiotic responses and promotes AM colonization of the host root. PMID:25527715

A superparamagnetic Fe{sub 3}O{sub 4}-graphene oxide nanocomposite for enrichment of nuciferine in the extract of Nelumbinis Folium (Lotus leaf)

Energy Technology Data Exchange (ETDEWEB)

Fan, Jie-Ping, E-mail: jasperfan@163.com [Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Department of Chemical Engineering, Nanchang University, Nanchang 330031 (China); Zheng, Bing; Qin, Yu; Yang, Dan; Liao, Dan-Dan; Xu, Xiao-Kang [Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Department of Chemical Engineering, Nanchang University, Nanchang 330031 (China); Zhang, Xue-Hong [School of Foreign Language, Nanchang University, Nanchang 330031 (China); Zhu, Jian-Hang [Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Department of Chemical Engineering, Nanchang University, Nanchang 330031 (China)

2016-02-28

Graphical abstract: - Highlights: • A superparamagnetic Fe3O4-graphene oxide (MGO) nanocomposite was prepared. • It is characterized by TEM, XPS, VSM, XRD and Raman spectroscopy. • The adsorption kinetics, isotherms and reusability of MGO were also investigated. • MGO was applied to enrich nuciferine in the extract of Nelumbinis Folium. - Abstract: In this work, a superparamagnetic Fe{sub 3}O{sub 4}-graphene oxide (MGO) nanocomposite was prepared by one-step chemical co-precipitation method, and characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), powder X-ray diffraction (PXRD), Raman spectroscopy and nitrogen adsorption–desorption curve. The as-prepared MGO was used to adsorb nuciferine, and the adsorption kinetic, isotherm and reusability of MGO were also investigated. The results showed that the adsorption of nuciferine on MGO reached its equilibrium very quickly (within 10 min) due to the two-dimensional carbon nanostructure of GO. In comparison with MGO, five conventional sorbents, i.e., macroporous resin D-101, silica gel, reverse phase silica gel (RP-C18) and cation exchange resin and polyamide, were also used to evaluate their adsorption capabilities. Therefore, MGO combined the advantages of both superparamagnetic particle and GO, i.e., easy separation and high absorption capacity. Finally, MGO was successfully applied to enrichment and separation of nuciferine in the extract of Nelumbinis Folium (Lotus leaf).

RNA-seq Transcriptional Profiling of an Arbuscular Mycorrhiza Provides Insights into Regulated and Coordinated Gene Expression in Lotus japonicus and Rhizophagus irregularis.

Science.gov (United States)

Handa, Yoshihiro; Nishide, Hiroyo; Takeda, Naoya; Suzuki, Yutaka; Kawaguchi, Masayoshi; Saito, Katsuharu

2015-08-01

Gene expression during arbuscular mycorrhizal development is highly orchestrated in both plants and arbuscular mycorrhizal fungi. To elucidate the gene expression profiles of the symbiotic association, we performed a digital gene expression analysis of Lotus japonicus and Rhizophagus irregularis using a HiSeq 2000 next-generation sequencer with a Cufflinks assembly and de novo transcriptome assembly. There were 3,641 genes differentially expressed during arbuscular mycorrhizal development in L. japonicus, approximately 80% of which were up-regulated. The up-regulated genes included secreted proteins, transporters, proteins involved in lipid and amino acid metabolism, ribosomes and histones. We also detected many genes that were differentially expressed in small-secreted peptides and transcription factors, which may be involved in signal transduction or transcription regulation during symbiosis. Co-regulated genes between arbuscular mycorrhizal and root nodule symbiosis were not particularly abundant, but transcripts encoding for membrane traffic-related proteins, transporters and iron transport-related proteins were found to be highly co-up-regulated. In transcripts of arbuscular mycorrhizal fungi, expansion of cytochrome P450 was observed, which may contribute to various metabolic pathways required to accommodate roots and soil. The comprehensive gene expression data of both plants and arbuscular mycorrhizal fungi provide a powerful platform for investigating the functional and molecular mechanisms underlying arbuscular mycorrhizal symbiosis. © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

Phytochemical Profile and Biological Activity of Nelumbo nucifera.

Science.gov (United States)

Paudel, Keshav Raj; Panth, Nisha

2015-01-01

Nelumbo nucifera Gaertn. (Nymphaeaceae) is a potential aquatic crop grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in various systems of medicine including folk medicines, Ayurveda, Chinese traditional medicine, and oriental medicine. Many chemical constituents have been isolated till the date. However, the bioactive constituents of lotus are mainly alkaloids and flavonoids. Traditionally, the whole plant of lotus was used as astringent, emollient, and diuretic. It was used in the treatment of diarrhea, tissue inflammation, and homeostasis. The rhizome extract was used as antidiabetic and anti-inflammatory properties due to the presence of asteroidal triterpenoid. Leaves were used as an effective drug for hematemesis, epistaxis, hemoptysis, hematuria, and metrorrhagia. Flowers were used to treat diarrhea, cholera, fever, and hyperdipsia. In traditional medicine practice, seeds are used in the treatment of tissue inflammation, cancer and skin diseases, leprosy, and poison antidote. Embryo of lotus seeds is used in traditional Chinese medicine as Lian Zi Xin, which primarily helps to overcome nervous disorders, insomnia, and cardiovascular diseases (hypertension and arrhythmia). Nutritional value of lotus is as important as pharmaceutical value. These days' different parts of lotus have been consumed as functional foods. Thus, lotus can be regarded as a potential nutraceutical source.

Phytochemical Profile and Biological Activity of Nelumbo nucifera

Directory of Open Access Journals (Sweden)

Keshav Raj Paudel

2015-01-01

Full Text Available Nelumbo nucifera Gaertn. (Nymphaeaceae is a potential aquatic crop grown and consumed throughout Asia. All parts of N. nucifera have been used for various medicinal purposes in various systems of medicine including folk medicines, Ayurveda, Chinese traditional medicine, and oriental medicine. Many chemical constituents have been isolated till the date. However, the bioactive constituents of lotus are mainly alkaloids and flavonoids. Traditionally, the whole plant of lotus was used as astringent, emollient, and diuretic. It was used in the treatment of diarrhea, tissue inflammation, and homeostasis. The rhizome extract was used as antidiabetic and anti-inflammatory properties due to the presence of asteroidal triterpenoid. Leaves were used as an effective drug for hematemesis, epistaxis, hemoptysis, hematuria, and metrorrhagia. Flowers were used to treat diarrhea, cholera, fever, and hyperdipsia. In traditional medicine practice, seeds are used in the treatment of tissue inflammation, cancer and skin diseases, leprosy, and poison antidote. Embryo of lotus seeds is used in traditional Chinese medicine as Lian Zi Xin, which primarily helps to overcome nervous disorders, insomnia, and cardiovascular diseases (hypertension and arrhythmia. Nutritional value of lotus is as important as pharmaceutical value. These days’ different parts of lotus have been consumed as functional foods. Thus, lotus can be regarded as a potential nutraceutical source.

Facility effluent monitoring plan determinations for the 400 Area facilities

International Nuclear Information System (INIS)

Nickels, J.M.

1991-09-01

This Facility Effluent Monitoring Plan determination resulted from an evaluation conducted for the Westinghouse Hanford Company 400 Area facilities on the Hanford Site. The Facility Effluent Monitoring Plan determinations have been prepared in accordance with A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans. Two major Westinghouse Hanford Company facilities in the 400 Area were evaluated: the Fast Flux Test Facility and the Fuels Manufacturing and examination Facility. The determinations were prepared by Westinghouse Hanford Company. Of these two facilities, only the Fast Flux Test Facility will require a Facility Effluent Monitoring Plan. 7 refs., 5 figs., 4 tabs

Dicty_cDB: Contig-U01335-1 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available 24 2.2 4 ( AW720069 ) LjNEST13d1r Lotus japonicus nodule library 5 and ... 36 2.2 2 ( AZ549980 ) ENTDD94TF Entamoeba histolytic...0053 Schistosoma manso... 36 3.3 2 ( BI417856 ) LjNEST37h10r Lotus japonicus nodule library 5 and... 36 3.4 ...2 ( BI417355 ) LjNEST38c2r Lotus japonicus nodule library 5 and ... 36 3.4 2 ( CG...ple Tissues, Normaliz... 34 3.5 2 ( CB828239 ) LjNEST84d3r Lotus japonicus nodule library 5 and ......077 3 ( CX198414 ) ydf29b11.y1 Sea urchin EST Lib1 Strongylocentrotu... 36 0.082 3 ( EE049942 ) zf_003_A10 Developmental library

Root growth of Lotus corniculatus interacts with P distribution in young sandy soil

Directory of Open Access Journals (Sweden)

B. Felderer

2013-03-01

Full Text Available Large areas of land are restored with unweathered soil substrates following mining activities in eastern Germany and elsewhere. In the initial stages of colonization of such land by vegetation, plant roots may become key agents in generating soil formation patterns by introducing gradients in chemical and physical soil properties. On the other hand, such patterns may be influenced by root growth responses to pre-existing substrate heterogeneities. In particular, the roots of many plants were found to preferentially proliferate into nutrient-rich patches. Phosphorus (P is of primary interest in this respect because its availability is often low in unweathered soils, limiting especially the growth of leguminous plants. However, leguminous plants occur frequently among the pioneer plant species on such soils, as they only depend on atmospheric nitrogen (N fixation as N source. In this study we investigated the relationship between root growth allocation of the legume Lotus corniculatus and soil P distribution on recently restored land. As test sites, the experimental Chicken Creek Catchment (CCC in eastern Germany and a nearby experimental site (ES with the same soil substrate were used. We established two experiments with constructed heterogeneity, one in the field on the experimental site and the other in a climate chamber. In addition, we conducted high-density samplings on undisturbed soil plots colonized by L. corniculatus on the ES and on the CCC. In the field experiment, we installed cylindrical ingrowth soil cores (4.5 × 10 cm with and without P fertilization around single two-month-old L. corniculatus plants. Roots showed preferential growth into the P-fertilized ingrowth-cores. Preferential root allocation was also found in the climate chamber experiment, where single L. corniculatus plants were grown in containers filled with ES soil and where a lateral portion of the containers was additionally supplied with a range of different P

From natural to biomimetic: The superhydrophobicity and the contact time.

Science.gov (United States)

Liang, Yun-Hong; Peng, Jian; Li, Xiu-Juan; Xu, Jin-Kai; Zhang, Zhi-Hui; Ren, Lu-Quan

2016-08-01

The superhydrophobicities and the contact time of lotus leaf and reed leaf were investigated. The results indicated that both lotus leaf and reed leaf have good superhydrophobic properties, and the water contact time was 12.7 and 14.7 ms on the surface of lotus leaf and reed leaf, respectively. Surface structure plays a key role in the different contacting times. Homogeneous distribution of papillae on the surface of lotus leaf was more helpful to reduce the contact time than anisotropic groove-shape on the surface of reed leaf. Based on the bionics coupling theory, the bionics sample possessing similar lotus-leaf-like surface structure on the aluminum alloy was designed and fabricated successfully. The water contact angle was about 153 ± 2°, sliding angle less than 5°, and the water contact time was 13.4 ms on the surface of bionics sample, which presented excellent superhydrophobic property, and achieved the aim of bionic design. Microsc. Res. Tech. 79:712-720, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Dance Facilities.

Science.gov (United States)

Ashton, Dudley, Ed.; Irey, Charlotte, Ed.

This booklet represents an effort to assist teachers and administrators in the professional planning of dance facilities and equipment. Three chapters present the history of dance facilities, provide recommended dance facilities and equipment, and offer some adaptations of dance facilities and equipment, for elementary, secondary and college level…

Facilities inventory protection for nuclear facilities

International Nuclear Information System (INIS)

Schmitt, F.J.

1989-01-01

The fact that shut-down applications have been filed for nuclear power plants, suggests to have a scrutinizing look at the scopes of assessment and decision available to administrations and courts for the protection of facilities inventories relative to legal and constitutional requirements. The paper outlines the legal bases which need to be observed if purposeful calculation is to be ensured. Based on the different actual conditions and legal consequences, the author distinguishes between 1) the legal situation of facilities licenced already and 2) the legal situation of facilities under planning during the licencing stage. As indicated by the contents and restrictions of the pertinent provisions of the Atomic Energy Act and by the corresponding compensatory regulation, the object of the protection of facilities inventor in the legal position of the facility owner within the purview of the Atomic Energy Act, and the licensing proper. Art. 17 of the Atomic Energy Act indicates the legislators intent that, once issued, the licence will be the pivotal point for regulations aiming at protection and intervention. (orig./HSCH) [de

Diospyros lotus leaf and grapefruit stem extract synergistically ameliorate atopic dermatitis-like skin lesion in mice by suppressing infiltration of mast cells in skin lesions.

Science.gov (United States)

Cho, Byoung Ok; Che, Denis Nchang; Yin, Hong Hua; Shin, Jae Young; Jang, Seon Il

2017-05-01

Atopic dermatitis, a chronic relapsing and pruritic inflammation of the skin also thought to be involved in, or caused by immune system destruction is an upsetting health problem due to its continuously increasing incidence especially in developed countries. Mast cell infiltration in atopic dermatitis skin lesions and its IgE-mediated activation releases various cytokines and chemokines that have been implicated in the pathogenesis of atopic dermatitis. This study was aimed at investigating synergistic anti-inflammatory, anti-pruritic and anti-atopic dermatitis effects of Diospyros lotus leaf extract (DLE) and Muscat bailey A grapefruit stem extract (GFSE) in atopic dermatitis-like induced skin lesions in mice. Combinations of DLE and GFSE inhibited TNF-α and IL-6 production more than DLE or GFSE in PMA plus calcium ionophore A23187-activated HMC-1 cells. DLE and GFSE synergistically inhibited compound 48/80-induced dermal infiltration of mast cells and reduced scratching behavior than DLE or GFSE. Furthermore, DLE and GFSE synergistically showed a stronger ameliorative effect in skin lesions by reducing clinical scores; dermal infiltration of mast cells; ear and dorsal skin thickness; serum IgE and IL-4 production in atopic dermatitis-like mice. Collectively, these results suggest that DLE and GFSE synergistically exhibit anti-atopic dermatitis effects in atopic dermatitis-like skin lesions in mice. Copyright © 2017. Published by Elsevier Masson SAS.

Facility effluent monitoring plan determinations for the 200 Area facilities

International Nuclear Information System (INIS)

Nickels, J.M.

1991-11-01

The following facility effluent monitoring plan determinations document the evaluations conducted for the Westinghouse Hanford Company 200 Area facilities (chemical processing, waste management, 222-S Laboratory, and laundry) on the Hanford Site in south central Washington State. These evaluations determined the need for facility effluent monitoring plans for the 200 Area facilities. The facility effluent monitoring plan determinations have been prepared in accordance with A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438 (WHC 1991). The Plutonium/Uranium Extraction Plant and UO 3 facility effluent monitoring plan determinations were prepared by Los Alamos Technical Associates, Richland, Washington. The Plutonium Finishing Plant, Transuranic Waste Storage and Assay Facility, T Plant, Tank Farms, Low Level Burial Grounds, and 222-S Laboratory determinations were prepared by Science Applications International Corporation of Richland, Washington. The B Plant Facility Effluent Monitoring Plan Determination was prepared by ERCE Environmental Services of Richland, Washington

Facilities & Leadership

Data.gov (United States)

Department of Veterans Affairs — The facilities web service provides VA facility information. The VA facilities locator is a feature that is available across the enterprise, on any webpage, for the...

Facility model for the Los Alamos Plutonium Facility

International Nuclear Information System (INIS)

Coulter, C.A.; Thomas, K.E.; Sohn, C.L.; Yarbro, T.F.; Hench, K.W.

1986-01-01

The Los Alamos Plutonium Facility contains more than sixty unit processes and handles a large variety of nuclear materials, including many forms of plutonium-bearing scrap. The management of the Plutonium Facility is supporting the development of a computer model of the facility as a means of effectively integrating the large amount of information required for material control, process planning, and facility development. The model is designed to provide a flexible, easily maintainable facility description that allows the faciltiy to be represented at any desired level of detail within a single modeling framework, and to do this using a model program and data files that can be read and understood by a technically qualified person without modeling experience. These characteristics were achieved by structuring the model so that all facility data is contained in data files, formulating the model in a simulation language that provides a flexible set of data structures and permits a near-English-language syntax, and using a description for unit processes that can represent either a true unit process or a major subsection of the facility. Use of the model is illustrated by applying it to two configurations of a fictitious nuclear material processing line

Animal facilities

International Nuclear Information System (INIS)

Fritz, T.E.; Angerman, J.M.; Keenan, W.G.; Linsley, J.G.; Poole, C.M.; Sallese, A.; Simkins, R.C.; Tolle, D.

1981-01-01

The animal facilities in the Division are described. They consist of kennels, animal rooms, service areas, and technical areas (examining rooms, operating rooms, pathology labs, x-ray rooms, and 60 Co exposure facilities). The computer support facility is also described. The advent of the Conversational Monitor System at Argonne has launched a new effort to set up conversational computing and graphics software for users. The existing LS-11 data acquisition systems have been further enhanced and expanded. The divisional radiation facilities include a number of gamma, neutron, and x-ray radiation sources with accompanying areas for related equipment. There are five 60 Co irradiation facilities; a research reactor, Janus, is a source for fission-spectrum neutrons; two other neutron sources in the Chicago area are also available to the staff for cell biology studies. The electron microscope facilities are also described

Tri-LORE-gy

DEFF Research Database (Denmark)

Mun, Terry

. This thesis presents the work behind the establishment of Lotus Base, which has seen wide use across the legume research community since its launch. The modular construction and scalable design of this online resource will also hopefully inspire and maybe benefit ongoing efforts to establish specialised...... secretion system (T4SS), but dependent on pathogen-encoded T3SS. Furthermore, LjEin2b was found in a symbiotic phenotyping screen to be required for negative regulation of symbiosis, but also confers susceptibility towards microbial wilt agents. In addition, a whole transcriptome sequencing experiment......, comprehensive online portal providing a user-friendly interface to access all Lotus data. The creation of Lotus Base (https://lotus.au.dk) is a response to a fragmented data landscape, helping to unify and integrated various datasets, and to provide simple yet powerful data visualisation and analysis tools...

Facilities Performance Indicators Report 2013-14: Tracking Your Facilities Vital Signs

Science.gov (United States)

APPA: Association of Higher Education Facilities Officers, 2015

2015-01-01

This paper features an expanded Web-based "Facilities Performance Indicators (FPI) Report." The purpose of APPA: Association of Higher Education Facilities Officers (APPA's) Facilities Performance Indicators is to provide a representative set of statistics about facilities in educational institutions. "The Facilities Performance…

Communication grounding facility

International Nuclear Information System (INIS)

Lee, Gye Seong

1998-06-01

It is about communication grounding facility, which is made up twelve chapters. It includes general grounding with purpose, materials thermal insulating material, construction of grounding, super strength grounding method, grounding facility with grounding way and building of insulating, switched grounding with No. 1A and LCR, grounding facility of transmission line, wireless facility grounding, grounding facility in wireless base station, grounding of power facility, grounding low-tenton interior power wire, communication facility of railroad, install of arrester in apartment and house, install of arrester on introduction and earth conductivity and measurement with introduction and grounding resistance.

Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis.

Science.gov (United States)

Fukudome, Mitsutaka; Calvo-Begueria, Laura; Kado, Tomohiro; Osuki, Ken-Ichi; Rubio, Maria Carmen; Murakami, Ei-Ichi; Nagata, Maki; Kucho, Ken-Ichi; Sandal, Niels; Stougaard, Jens; Becana, Manuel; Uchiumi, Toshiki

2016-09-01

Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia-legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5'-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels. © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Facility effluent monitoring plan for the 327 Facility

International Nuclear Information System (INIS)

1994-11-01

The 327 Facility [Post-Irradiation Testing Laboratory] provides office and laboratory space for Pacific Northwest Laboratory (PNL) scientific and engineering staff conducting multidisciplinary research in the areas of post-irradiated fuels and structural materials. The facility is designed to accommodate the use of radioactive and hazardous materials in the conduct of these activities. This report summarizes the airborne emissions and liquid effluents and the results of the Facility Effluent Monitoring Plan (FEMP) determination for the facility. The complete monitoring plan includes characterization of effluent streams, monitoring/sampling design criteria, a description of the monitoring systems and sample analysis, and quality assurance requirements

Facility effluent monitoring plan for the 325 Facility

International Nuclear Information System (INIS)

1998-01-01

The Applied Chemistry Laboratory (325 Facility) houses radiochemistry research, radioanalytical service, radiochemical process development, and hazardous and mixed hazardous waste treatment activities. The laboratories and specialized facilities enable work ranging from that with nonradioactive materials to work with picogram to kilogram quantities of fissionable materials and up to megacurie quantities of other radionuclides. The special facilities include two shielded hot-cell areas that provide for process development or analytical chemistry work with highly radioactive materials, and a waste treatment facility for processing hazardous, mixed, low-level, and transuranic wastes generated by Pacific Northwest Laboratory. Radioactive material storage and usage occur throughout the facility and include a large number of isotopes. This material is in several forms, including solid, liquid, particulate, and gas. Some of these materials are also heated during testing which can produce vapors. The research activities have been assigned to the following activity designations: High-Level Hot Cell, Hazardous Waste Treatment Unit, Waste Form Development, Special Testing Projects, Chemical Process Development, Analytical Hot Cell, and Analytical Chemistry. The following summarizes the airborne and liquid effluents and the results of the Facility Effluent Monitoring Plan (FEMP) determination for the facility. The complete monitoring plan includes characterization of effluent streams, monitoring/sampling design criteria, a description of the monitoring systems and sample analysis, and quality assurance requirements

Air Quality Facilities

Data.gov (United States)

Iowa State University GIS Support and Research Facility — Facilities with operating permits for Title V of the Federal Clean Air Act, as well as facilities required to submit an air emissions inventory, and other facilities...

Facilities Performance Indicators Report, 2004-05. Facilities Core Data Survey

Science.gov (United States)

Glazner, Steve, Ed.

2006-01-01

The purpose of "Facilities Performance Indicators" is to provide a representative set of statistics about facilities in educational institutions. The second iteration of the web-based Facilities Core Data Survey was posted and available to facilities professionals at more than 3,000 institutions in the Fall of 2005. The website offered a printed…

High-risk facilities. Emergency management in nuclear, chemical and hazardous waste facilities

International Nuclear Information System (INIS)

Kloepfer, Michael

2012-01-01

The book on emergency management in high-risk facilities covers the following topics: Change in the nuclear policy, risk management of high-risk facilities as a constitutional problem - emergency management in nuclear facilities, operational mechanisms of risk control in nuclear facilities, regulatory surveillance responsibilities for nuclear facilities, operational mechanism of the risk control in chemical plants, regulatory surveillance responsibilities for chemical facilities, operational mechanisms of the risk control in hazardous waste facilities, regulatory surveillance responsibilities for hazardous waste facilities, civil law consequences in case of accidents in high-risk facilities, criminal prosecution in case of accidents in high-risk facilities, safety margins as site risk for emission protection facilities, national emergency management - strategic emergency management structures, warning and self-protection of the public in case of CBRN hazards including aspects of the psych-social emergency management.

Nuclear facilities

International Nuclear Information System (INIS)

Anon.

2000-01-01

Here is given the decree (2000-1065) of the 25. of October 2000 reporting the publication of the convention between the Government of the French Republic and the CERN concerning the safety of the LHC (Large Hadron Collider) and the SPS (Proton Supersynchrotron) facilities, signed in Geneva on July 11, 2000. By this convention, the CERN undertakes to ensure the safety of the LHC and SPS facilities and those of the operations of the LEP decommissioning. The French legislation and regulations on basic nuclear facilities (concerning more particularly the protection against ionizing radiations, the protection of the environment and the safety of facilities) and those which could be decided later on apply to the LHC, SPS and auxiliary facilities. (O.M.)

Facility effluent monitoring plan for 242-A Evaporator facility

International Nuclear Information System (INIS)

Crummel, G.M.; Gustavson, R.D.

1993-03-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1* for any operations that involve hazardous materials and radioactive substances that could affect employee or public safety or the environment. A facility effluent monitoring plan determination was performed during Calendar Year 1991 and the evaluation showed the need for a facility effluent monitoring plan. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility effluent Monitoring Plans, WHC-EP-0438-1**. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements

North Slope, Alaska ESI: FACILITY (Facility Points)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains data for oil field facilities for the North Slope of Alaska. Vector points in this data set represent oil field facility locations. This data...

26 CFR 1.142(a)(5)-1 - Exempt facility bonds: Sewage facilities.

Science.gov (United States)

2010-04-01

... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Exempt facility bonds: Sewage facilities. 1.142... Bonds § 1.142(a)(5)-1 Exempt facility bonds: Sewage facilities. (a) In general. Under section 103(a), a... in section 142(a) is a sewage facility. This section defines the term sewage facility for purposes of...

Facility transition instruction

International Nuclear Information System (INIS)

Morton, M.R.

1997-01-01

The Bechtel Hanford, Inc. facility transition instruction was initiated in response to the need for a common, streamlined process for facility transitions and to capture the knowledge and experience that has accumulated over the last few years. The instruction serves as an educational resource and defines the process for transitioning facilities to long-term surveillance and maintenance (S and M). Generally, these facilities do not have identified operations missions and must be transitioned from operational status to a safe and stable configuration for long-term S and M. The instruction can be applied to a wide range of facilities--from process canyon complexes like the Plutonium Uranium Extraction Facility or B Plant, to stand-alone, lower hazard facilities like the 242B/BL facility. The facility transition process is implemented (under the direction of the US Department of Energy, Richland Operations Office [RL] Assistant Manager-Environmental) by Bechtel Hanford, Inc. management, with input and interaction with the appropriate RL division and Hanford site contractors as noted in the instruction. The application of the steps identified herein and the early participation of all organizations involved are expected to provide a cost-effective, safe, and smooth transition from operational status to deactivation and S and M for a wide range of Hanford Site facilities

Facilities Performance Indicators Report 2012-13: Tracking Your Facilities Vital Signs

Science.gov (United States)

APPA: Association of Higher Education Facilities Officers, 2014

2014-01-01

This paper features an expanded Web-based "Facilities Performance Indicators (FPI) Report." The purpose of APPA's Facilities Performance Indicators is to provide a representative set of statistics about facilities in educational institutions. "The Facilities Performance Indicators Report" is designed for survey…

Guide to research facilities

Energy Technology Data Exchange (ETDEWEB)

1993-06-01

This Guide provides information on facilities at US Department of Energy (DOE) and other government laboratories that focus on research and development of energy efficiency and renewable energy technologies. These laboratories have opened these facilities to outside users within the scientific community to encourage cooperation between the laboratories and the private sector. The Guide features two types of facilities: designated user facilities and other research facilities. Designated user facilities are one-of-a-kind DOE facilities that are staffed by personnel with unparalleled expertise and that contain sophisticated equipment. Other research facilities are facilities at DOE and other government laboratories that provide sophisticated equipment, testing areas, or processes that may not be available at private facilities. Each facility listing includes the name and phone number of someone you can call for more information.

Experimental Fuels Facility Re-categorization Based on Facility Segmentation

Energy Technology Data Exchange (ETDEWEB)

Reiss, Troy P.; Andrus, Jason

2016-07-01

The Experimental Fuels Facility (EFF) (MFC-794) at the Materials and Fuels Complex (MFC) located on the Idaho National Laboratory (INL) Site was originally constructed to provide controlled-access, indoor storage for radiological contaminated equipment. Use of the facility was expanded to provide a controlled environment for repairing contaminated equipment and characterizing, repackaging, and treating waste. The EFF facility is also used for research and development services, including fuel fabrication. EFF was originally categorized as a LTHC-3 radiological facility based on facility operations and facility radiological inventories. Newly planned program activities identified the need to receive quantities of fissionable materials in excess of the single parameter subcritical limit in ANSI/ANS-8.1, “Nuclear Criticality Safety in Operations with Fissionable Materials Outside Reactors” (identified as “criticality list” quantities in DOE-STD-1027-92, “Hazard Categorization and Accident Analysis Techniques for Compliance with DOE Order 5480.23, Nuclear Safety Analysis Reports,” Attachment 1, Table A.1). Since the proposed inventory of fissionable materials inside EFF may be greater than the single parameter sub-critical limit of 700 g of U-235 equivalent, the initial re-categorization is Hazard Category (HC) 2 based upon a potential criticality hazard. This paper details the facility hazard categorization performed for the EFF. The categorization was necessary to determine (a) the need for further safety analysis in accordance with LWP-10802, “INL Facility Categorization,” and (b) compliance with 10 Code of Federal Regulations (CFR) 830, Subpart B, “Safety Basis Requirements.” Based on the segmentation argument presented in this paper, the final hazard categorization for the facility is LTHC-3. Department of Energy Idaho (DOE-ID) approval of the final hazard categorization determined by this hazard assessment document (HAD) was required per the

Hanford Facility dangerous waste permit application, liquid effluent retention facility and 200 area effluent treatment facility

International Nuclear Information System (INIS)

Coenenberg, J.G.

1997-01-01

The Hanford Facility Dangerous Waste Permit Application is considered to 10 be a single application organized into a General Information Portion (document 11 number DOE/RL-91-28) and a Unit-Specific Portion. The scope of the 12 Unit-Specific Portion is limited to Part B permit application documentation 13 submitted for individual, 'operating' treatment, storage, and/or disposal 14 units, such as the Liquid Effluent Retention Facility and 200 Area Effluent 15 Treatment Facility (this document, DOE/RL-97-03). 16 17 Both the General Information and Unit-Specific portions of the Hanford 18 Facility Dangerous Waste Permit Application address the content of the Part B 19 permit application guidance prepared by the Washington State Department of 20 Ecology (Ecology 1987 and 1996) and the U.S. Environmental Protection Agency 21 (40 Code of Federal Regulations 270), with additional information needs 22 defined by the Hazardous and Solid Waste Amendments and revisions of 23 Washington Administrative Code 173-303. For ease of reference, the Washington 24 State Department of Ecology alpha-numeric section identifiers from the permit 25 application guidance documentation (Ecology 1996) follow, in brackets, the 26 chapter headings and subheadings. A checklist indicating where information is 27 contained in the Liquid Effluent Retention Facility and 200 Area Effluent 28 Treatment Facility permit application documentation, in relation to the 29 Washington State Department of Ecology guidance, is located in the Contents 30 Section. 31 32 Documentation contained in the General Information Portion is broader in 33 nature and could be used by multiple treatment, storage, and/or disposal units 34 (e.g., the glossary provided in the General Information Portion). Wherever 35 appropriate, the Liquid Effluent Retention Facility and 200 Area Effluent 36 Treatment Facility permit application documentation makes cross-reference to 37 the General Information Portion, rather than duplicating

Hanford Facility dangerous waste permit application, liquid effluent retention facility and 200 area effluent treatment facility

Energy Technology Data Exchange (ETDEWEB)

Coenenberg, J.G.

1997-08-15

The Hanford Facility Dangerous Waste Permit Application is considered to 10 be a single application organized into a General Information Portion (document 11 number DOE/RL-91-28) and a Unit-Specific Portion. The scope of the 12 Unit-Specific Portion is limited to Part B permit application documentation 13 submitted for individual, `operating` treatment, storage, and/or disposal 14 units, such as the Liquid Effluent Retention Facility and 200 Area Effluent 15 Treatment Facility (this document, DOE/RL-97-03). 16 17 Both the General Information and Unit-Specific portions of the Hanford 18 Facility Dangerous Waste Permit Application address the content of the Part B 19 permit application guidance prepared by the Washington State Department of 20 Ecology (Ecology 1987 and 1996) and the U.S. Environmental Protection Agency 21 (40 Code of Federal Regulations 270), with additional information needs 22 defined by the Hazardous and Solid Waste Amendments and revisions of 23 Washington Administrative Code 173-303. For ease of reference, the Washington 24 State Department of Ecology alpha-numeric section identifiers from the permit 25 application guidance documentation (Ecology 1996) follow, in brackets, the 26 chapter headings and subheadings. A checklist indicating where information is 27 contained in the Liquid Effluent Retention Facility and 200 Area Effluent 28 Treatment Facility permit application documentation, in relation to the 29 Washington State Department of Ecology guidance, is located in the Contents 30 Section. 31 32 Documentation contained in the General Information Portion is broader in 33 nature and could be used by multiple treatment, storage, and/or disposal units 34 (e.g., the glossary provided in the General Information Portion). Wherever 35 appropriate, the Liquid Effluent Retention Facility and 200 Area Effluent 36 Treatment Facility permit application documentation makes cross-reference to 37 the General Information Portion, rather than duplicating

Facility effluent monitoring plan for 242-A evaporator facility

International Nuclear Information System (INIS)

Crummel, G.M.; Gustavson, R.D.

1995-02-01

A facility effluent monitoring plan is required by the U.S. Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could affect employee or public safety or the environment. A facility effluent monitoring plan determination was performed during Calendar Year 1991 and the evaluation showed the need for a facility effluent monitoring plan. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-1. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated, as a minimum, every three years

EPA Facility Registry Service (FRS): Facility Interests Dataset

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service consists of location and facility identification information from EPA's Facility Registry Service (FRS) for all sites that are available in...

Hydrocarbon degradation potential and plant growth-promoting activity of culturable endophytic bacteria of Lotus corniculatus and Oenothera biennis from a long-term polluted site.

Science.gov (United States)

Pawlik, Małgorzata; Cania, Barbara; Thijs, Sofie; Vangronsveld, Jaco; Piotrowska-Seget, Zofia

2017-08-01

Many endophytic bacteria exert beneficial effects on their host, but still little is known about the bacteria associated with plants growing in areas heavily polluted by hydrocarbons. The aim of the study was characterization of culturable hydrocarbon-degrading endophytic bacteria associated with Lotus corniculatus L. and Oenothera biennis L. collected in long-term petroleum hydrocarbon-polluted site using culture-dependent and molecular approaches. A total of 26 hydrocarbon-degrading endophytes from these plants were isolated. Phylogenetic analyses classified the isolates into the phyla Proteobacteria and Actinobacteria. The majority of strains belonged to the genera Rhizobium, Pseudomonas, Stenotrophomonas, and Rhodococcus. More than 90% of the isolates could grow on medium with diesel oil, approximately 20% could use n-hexadecane as a sole carbon and energy source. PCR analysis revealed that 40% of the isolates possessed the P450 gene encoding for cytochrome P450-type alkane hydroxylase (CYP153). In in vitro tests, all endophytic strains demonstrated a wide range of plant growth-promoting traits such as production of indole-3-acetic acid, hydrogen cyanide, siderophores, and phosphate solubilization. More than 40% of the bacteria carried the gene encoding for the 1-aminocyclopropane-1-carboxylic acid deaminase (acdS). Our study shows that the diversity of endophytic bacterial communities in tested plants was different. The results revealed also that the investigated plants were colonized by endophytic bacteria possessing plant growth-promoting features and a clear potential to degrade hydrocarbons. The properties of isolated endophytes indicate that they have the high potential to improve phytoremediation of petroleum hydrocarbon-polluted soils.

Forward and reverse genetics: The LORE1 retrotransposon insertion mutants

DEFF Research Database (Denmark)

Fukai, Eigo; Malolepszy, Anna; Sandal, Niels Nørgaard

2014-01-01

The endogenous Lotus retrotransposon 1 (LORE1) transposes in the germ line of Lotus japonicus plants that carry an active element. This feature of LORE1 has been exploited for generation of a large non-transgenic insertion mutant population, where insertions have been annotated using next-generat...

Biochemistry Facility

Data.gov (United States)

Federal Laboratory Consortium — The Biochemistry Facility provides expert services and consultation in biochemical enzyme assays and protein purification. The facility currently features 1) Liquid...

Facility effluent monitoring plan for the plutonium-uranium extraction facility

International Nuclear Information System (INIS)

Nickels, J.M.; Geiger, J.L.

1992-11-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. A facility effluent monitoring plan determination was performed during Calendar Year 1991 and the evaluation requires the need for a facility effluent monitoring plan. This document is prepared using the specific guidelines identified. in. A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements

Jupiter Laser Facility

Data.gov (United States)

Federal Laboratory Consortium — The Jupiter Laser Facility is an institutional user facility in the Physical and Life Sciences Directorate at LLNL. The facility is designed to provide a high degree...

Liquid Effluent Retention Facility/Effluent Treatment Facility Hazards Assessment

International Nuclear Information System (INIS)

Simiele, G.A.

1994-01-01

This document establishes the technical basis in support of Emergency Planning activities for the Liquid Effluent Retention Facility and Effluent Treatment Facility the Hanford Site. The document represents an acceptable interpretation of the implementing guidance document for DOE ORDER 5500.3A. Through this document, the technical basis for the development of facility specific Emergency Action Levels and the Emergency Planning Zone is demonstrated

Research Facilities | Wind | NREL

Science.gov (United States)

Research Facilities Research Facilities NREL's state-of-the-art wind research facilities at the Research Facilities Photo of five men in hard hards observing the end of a turbine blade while it's being tested. Structural Research Facilities A photo of two people silhouetted against a computer simulation of

Waste Facilities

Data.gov (United States)

Vermont Center for Geographic Information — This dataset was developed from the Vermont DEC's list of certified solid waste facilities. It includes facility name, contact information, and the materials...

Fabrication Facilities

Data.gov (United States)

Federal Laboratory Consortium — The Fabrication Facilities are a direct result of years of testing support. Through years of experience, the three fabrication facilities (Fort Hood, Fort Lewis, and...

Applied bioinformatics: Genome annotation and transcriptome analysis

DEFF Research Database (Denmark)

Gupta, Vikas

agricultural and biological importance. Its capacity to form symbiotic relationships with rhizobia and microrrhizal fungi has fascinated researchers for years. Lotus has a small genome of approximately 470 Mb and a short life cycle of 2 to 3 months, which has made Lotus a model legume plant for many molecular...

EPA Facility Registry Service (FRS): Facility Interests Dataset Download

Data.gov (United States)

U.S. Environmental Protection Agency — This downloadable data package consists of location and facility identification information from EPA's Facility Registry Service (FRS) for all sites that are...

Finding privacy from a public death: a qualitative exploration of how a dedicated space for end-of-life care in an acute hospital impacts on dying patients and their families.

Science.gov (United States)

Slatyer, Susan; Pienaar, Catherine; Williams, Anne M; Proctor, Karen; Hewitt, Laura

2015-08-01

To explore the experiences and perceptions of hospital staff caring for dying patients in a dedicated patient/family room (named Lotus Room). Dying in hospital is a common outcome for people across the world. However, noise and activity in acute environments present barriers to quality end-of-life care. This is of concern because care provided to dying patients has been shown to affect both the patients and the bereaved families. A qualitative descriptive approach was used. Semi-structured interviews were conducted with 17 multidisciplinary staff and seven families provided information through an investigator-developed instrument. Qualitative data analysis generated three categories describing: Dying in an hospital; The Lotus Room; and the Outcomes for patients and families. The Lotus Room was seen as a large, private and, ultimately, safe space for patients and families within the public hospital environment. Family feedback supported staff perspectives that the Lotus Room facilitated family presence and communication. The privacy afforded by the Lotus Room within this acute hospital provided benefits for the dying patients and grieving families. Improved outcomes included a peaceful death for patients, which may have assisted the family with their bereavement. This study provides evidence of how the physical environment can address well-established barriers to quality end-of-life care in acute hospitals. © 2015 John Wiley & Sons Ltd.

Irradiation Facilities at CERN

CERN Document Server

Gkotse, Blerina; Carbonez, Pierre; Danzeca, Salvatore; Fabich, Adrian; Garcia, Alia, Ruben; Glaser, Maurice; Gorine, Georgi; Jaekel, Martin, Richard; Mateu,Suau, Isidre; Pezzullo, Giuseppe; Pozzi, Fabio; Ravotti, Federico; Silari, Marco; Tali, Maris

2017-01-01

CERN provides unique irradiation facilities for applications in many scientific fields. This paper summarizes the facilities currently operating for proton, gamma, mixed-field and electron irradiations, including their main usage, characteristics and information about their operation. The new CERN irradiation facilities database is also presented. This includes not only CERN facilities but also irradiation facilities available worldwide.

EPA Facility Registry Service (FRS): Facility Interests Dataset - Intranet

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service consists of location and facility identification information from EPA's Facility Registry Service (FRS) for all sites that are available in...

Hanford Facility Dangerous Waste Permit Application, 200 Area Effluent Treatment Facility

International Nuclear Information System (INIS)

1993-08-01

The 200 Area Effluent Treatment Facility Dangerous Waste Permit Application documentation consists of both Part A and a Part B permit application documentation. An explanation of the Part A revisions associated with this treatment and storage unit, including the current revision, is provided at the beginning of the Part A section. Once the initial Hanford Facility Dangerous Waste Permit is issued, the following process will be used. As final, certified treatment, storage, and/or disposal unit-specific documents are developed, and completeness notifications are made by the US Environmental Protection Agency and the Washington State Department of Ecology, additional unit-specific permit conditions will be incorporated into the Hanford Facility Dangerous Waste Permit through the permit modification process. All treatment, storage, and/or disposal units that are included in the Hanford Facility Dangerous Waste Permit Application will operate under interim status until final status conditions for these units are incorporated into the Hanford Facility Dangerous Waste Permit. The Hanford Facility Dangerous Waste Permit Application, 200 Area Effluent Treatment Facility contains information current as of May 1, 1993

Hanford Facility Dangerous Waste Permit Application, 200 Area Effluent Treatment Facility

Energy Technology Data Exchange (ETDEWEB)

1993-08-01

The 200 Area Effluent Treatment Facility Dangerous Waste Permit Application documentation consists of both Part A and a Part B permit application documentation. An explanation of the Part A revisions associated with this treatment and storage unit, including the current revision, is provided at the beginning of the Part A section. Once the initial Hanford Facility Dangerous Waste Permit is issued, the following process will be used. As final, certified treatment, storage, and/or disposal unit-specific documents are developed, and completeness notifications are made by the US Environmental Protection Agency and the Washington State Department of Ecology, additional unit-specific permit conditions will be incorporated into the Hanford Facility Dangerous Waste Permit through the permit modification process. All treatment, storage, and/or disposal units that are included in the Hanford Facility Dangerous Waste Permit Application will operate under interim status until final status conditions for these units are incorporated into the Hanford Facility Dangerous Waste Permit. The Hanford Facility Dangerous Waste Permit Application, 200 Area Effluent Treatment Facility contains information current as of May 1, 1993.

EPA Facility Registry Service (FRS): AIRS_AFS Sub Facilities

Data.gov (United States)

U.S. Environmental Protection Agency — The Air Facility System (AFS) contains compliance and permit data for stationary sources regulated by EPA, state and local air pollution agencies. The sub facility...

Facility effluent monitoring plan for the fast flux test facility

International Nuclear Information System (INIS)

Nickels, J.M.; Dahl, N.R.

1992-11-01

A facility effluent monitoring plan is required by the US Department of Energy in US Department of Energy Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could affect employee or public safety or the environment. A Facility Effluent Monitoring Plan determination was performed during calendar year 1991 and the evaluation requires the need for a facility effluent monitoring plan. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements

DUPIC facility engineering

Energy Technology Data Exchange (ETDEWEB)

Lee, J S; Choi, J W; Go, W I; Kim, H D; Song, K C; Jeong, I H; Park, H S; Im, C S; Lee, H M; Moon, K H; Hong, K P; Lee, K S; Suh, K S; Kim, E K; Min, D K; Lee, J C; Chun, Y B; Paik, S Y; Lee, E P; Yoo, G S; Kim, Y S; Park, J C

1997-09-01

In the early stage of the project, a comprehensive survey was conducted to identify the feasibility of using available facilities and of interface between those facilities. It was found out that the shielded cell M6 interface between those facilities. It was found out that the shielded cell M6 of IMEF could be used for the main process experiments of DUPIC fuel fabrication in regard to space adequacy, material flow, equipment layout, etc. Based on such examination, a suitable adapter system for material transfer around the M6 cell was engineered. Regarding the PIEF facility, where spent PWR fuel assemblies are stored in an annex pool, disassembly devices in the pool are retrofitted and spent fuel rod cutting and shipping system to the IMEF are designed and built. For acquisition of casks for radioactive material transport between the facilities, some adaptive refurbishment was applied to the available cask (Padirac) based on extensive analysis on safety requirements. A mockup test facility was newly acquired for remote test of DUPIC fuel fabrication process equipment prior to installation in the M6 cell of the IMEF facility. (author). 157 refs., 57 tabs., 65 figs.

DUPIC facility engineering

International Nuclear Information System (INIS)

Lee, J. S.; Choi, J. W.; Go, W. I.; Kim, H. D.; Song, K. C.; Jeong, I. H.; Park, H. S.; Im, C. S.; Lee, H. M.; Moon, K. H.; Hong, K. P.; Lee, K. S.; Suh, K. S.; Kim, E. K.; Min, D. K.; Lee, J. C.; Chun, Y. B.; Paik, S. Y.; Lee, E. P.; Yoo, G. S.; Kim, Y. S.; Park, J. C.

1997-09-01

In the early stage of the project, a comprehensive survey was conducted to identify the feasibility of using available facilities and of interface between those facilities. It was found out that the shielded cell M6 interface between those facilities. It was found out that the shielded cell M6 of IMEF could be used for the main process experiments of DUPIC fuel fabrication in regard to space adequacy, material flow, equipment layout, etc. Based on such examination, a suitable adapter system for material transfer around the M6 cell was engineered. Regarding the PIEF facility, where spent PWR fuel assemblies are stored in an annex pool, disassembly devices in the pool are retrofitted and spent fuel rod cutting and shipping system to the IMEF are designed and built. For acquisition of casks for radioactive material transport between the facilities, some adaptive refurbishment was applied to the available cask (Padirac) based on extensive analysis on safety requirements. A mockup test facility was newly acquired for remote test of DUPIC fuel fabrication process equipment prior to installation in the M6 cell of the IMEF facility. (author). 157 refs., 57 tabs., 65 figs

Health Facilities

Science.gov (United States)

Health facilities are places that provide health care. They include hospitals, clinics, outpatient care centers, and specialized care centers, ... psychiatric care centers. When you choose a health facility, you might want to consider How close it ...

DUPIC facility engineering

Energy Technology Data Exchange (ETDEWEB)

Park, J. J.; Lee, H. H.; Kim, K. H. and others

2000-03-01

The objectives of this study are (1) the refurbishment for PIEF(Post Irradiation Examination Facility) and M6 hot-cell in IMEF(Irradiated Material Examination Facility), (2) the establishment of the compatible facility for DUPIC fuel fabrication experiments which is licensed by government organization, and (3) the establishment of the transportation system and transportation cask for nuclear material between facilities. The report for this project describes following contents, such as objectives, necessities, scope, contents, results of current step, R and D plan in future and etc.

An Ornithopod-Dominated Tracksite from the Lower Cretaceous Jiaguan Formation (Barremian-Albian) of Qijiang, South-Central China: New Discoveries, Ichnotaxonomy, Preservation and Palaeoecology.

Science.gov (United States)

Xing, Lida; Lockley, Martin G; Marty, Daniel; Zhang, Jianping; Wang, Yan; Klein, Hendrik; McCrea, Richard T; Buckley, Lisa G; Belvedere, Matteo; Mateus, Octávio; Gierliński, Gerard D; Piñuela, Laura; Persons, W Scott; Wang, Fengping; Ran, Hao; Dai, Hui; Xie, Xianming

2015-01-01

The historically-famous Lotus Fortress site, a deep 1.5-3.0-meter-high, 200-meter-long horizonal notch high up in near-vertical sandstone cliffs comprising the Cretaceous Jiaguan Formation, has been known since the 13th Century as an impregnable defensive position. The site is also extraordinary for having multiple tetrapod track-bearing levels, of which the lower two form the floor of part of the notch, and yield very well preserved asseamblages of ornithopod, bird (avian theropod) and pterosaur tracks. Trackway counts indicate that ornithopods dominate (69%) accounting for at least 165 trackmakers, followed by bird (18%), sauropod (10%), and pterosaur (3%). Previous studies designated Lotus Fortress as the type locality of Caririchnium lotus and Wupus agilis both of which are recognized here as valid ichnotaxa. On the basis of multiple parallel trackways both are interpreted as representing the trackways of gregarious species. C. lotus is redescribed here in detail and interpreted to indicate two age cohorts representing subadults that were sometimes bipedal and larger quadrupedal adults. Two other previously described dinosaurian ichnospecies, are here reinterpreted as underprints and considered nomina dubia. Like a growing number of significant tetrapod tracksites in China the Lotus Fortress site reveals new information about the composition of tetrapod faunas from formations in which the skeletal record is sparse. In particular, the site shows the relatively high abundance of Caririchium in a region where saurischian ichnofaunas are often dominant. It is also the only site known to have yielded Wupus agilis. In combination with information from other tracksites from the Jiaguan formation and other Cretaceous formations in the region, the track record is proving increasingly impotant as a major source of information on the vertebrate faunas of the region. The Lotus Fortress site has been developed as a spectacular, geologically-, paleontologically- and a

Shielding of Medical Facilities. Shielding Design Considerations for PET-CT Facilities

International Nuclear Information System (INIS)

Cruzate, J.A.; Discacciatti, A.P.

2011-01-01

The radiological evaluation of a Positron Emission Tomography (PET) facility consists of the assessment of the annual effective dose both to workers occupationally exposed, and to members of the public. This assessment takes into account the radionuclides involved, the facility features, the working procedures, the expected number of patients per year, and so on. The evaluation embraces the distributions of rooms, the thickness and physical material of walls, floors and ceilings. This work detail the methodology used for making the assessment of a PET facility design taking into account only radioprotection aspects. The assessment results must be compared to the design requirements established by national regulations in order to determine whether or not, the facility complies with those requirements, both for workers and for members of the public. The analysis presented is useful for both, facility designers and regulators. In addition, some guidelines for improving the shielding design and working procedures are presented in order to help facility designer's job. (authors)

Energy Systems Integration Facility (ESIF) Facility Stewardship Plan: Revision 2.1

Energy Technology Data Exchange (ETDEWEB)

Torres, Juan [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Anderson, Art [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

2018-01-02

The U.S. Department of Energy (DOE), Office of Energy Efficiency and Renewable Energy (EERE), has established the Energy Systems Integration Facility (ESIF) on the campus of the National Renewable Energy Laboratory (NREL) and has designated it as a DOE user facility. This 182,500-ft2 research facility provides state-of-the-art laboratory and support infrastructure to optimize the design and performance of electrical, thermal, fuel, and information technologies and systems at scale. This Facility Stewardship Plan provides DOE and other decision makers with information about the existing and expected capabilities of the ESIF and the expected performance metrics to be applied to ESIF operations. This plan is a living document that will be updated and refined throughout the lifetime of the facility.

Facilities Performance Indicators Report 2011-12: Tracking Your Facilities Vital Signs

Science.gov (United States)

APPA: Association of Higher Education Facilities Officers, 2013

2013-01-01

This paper provides an expanded Web-based "Facilities Performance Indicators (FPI) Report." The purpose of APPA's Facilities Performance Indicators is to provide a representative set of statistics about facilities in educational institutions. APPA's Information and Research Committee's goal for this year was to enhance the…

Facility effluent monitoring plan for the tank farm facility

Energy Technology Data Exchange (ETDEWEB)

Crummel, G.M.

1998-05-18

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements.

CLEAR test facility

CERN Multimedia

Ordan, Julien Marius

2017-01-01

A new user facility for accelerator R&D, the CERN Linear Electron Accelerator for Research (CLEAR), started operation in August 2017. CLEAR evolved from the former CLIC Test Facility 3 (CTF3) used by the Compact Linear Collider (CLIC). The new facility is able to host and test a broad range of ideas in the accelerator field.

Facility Effluent Monitoring Plan for Pacific Northwest National Laboratory Balance-of-Plant Facilities

Energy Technology Data Exchange (ETDEWEB)

Ballinger, Marcel Y.; Gervais, Todd L.

2004-11-15

The Pacific Northwest National Laboratory (PNNL) operates a number of Research & Development (R&D) facilities for the U.S. Department of Energy (DOE) on the Hanford Site. Facility effluent monitoring plans (FEMPs) have been developed to document the facility effluent monitoring portion of the Environmental Monitoring Plan (DOE 2000) for the Hanford Site. Three of PNNL’s R&D facilities, the 325, 331, and 3720 Buildings, are considered major emission points for radionuclide air sampling, and individual FEMPs were developed for these facilities in the past. In addition, a balance-of-plant (BOP) FEMP was developed for all other DOE-owned, PNNL-operated facilities at the Hanford Site. Recent changes, including shutdown of buildings and transition of PNNL facilities to the Office of Science, have resulted in retiring the 3720 FEMP and combining the 331 FEMP into the BOP FEMP. This version of the BOP FEMP addresses all DOE-owned, PNNL-operated facilities at the Hanford Site, excepting the Radiochemical Processing Laboratory, which has its own FEMP because of the unique nature of the building and operations. Activities in the BOP facilities range from administrative to laboratory and pilot-scale R&D. R&D activities include both radioactive and chemical waste characterization, fluid dynamics research, mechanical property testing, dosimetry research, and molecular sciences. The mission and activities for individual buildings are described in Appendix A. Potential radioactive airborne emissions in the BOP facilities are estimated annually using a building inventory-based approach provided in federal regulations. Sampling at individual BOP facilities is based on a potential-to-emit assessment. Some of these facilities are considered minor emission points and thus are sampled routinely, but not continuously, to confirm the low emission potential. One facility, the 331 Life Sciences Laboratory, has a major emission point and is sampled continuously. Sampling systems are

EPA Facility Registry Service (FRS): Facility Interests Dataset - Intranet Download

Data.gov (United States)

U.S. Environmental Protection Agency — This downloadable data package consists of location and facility identification information from EPA's Facility Registry Service (FRS) for all sites that are...

Facility Effluent Monitoring Plan for the uranium trioxide facility

International Nuclear Information System (INIS)

Lohrasbi, J.; Johnson, D.L.; De Lorenzo, D.S.

1993-12-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-01. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated at a minimum of every three years

EPA Facility Registry Service (FRS): Facility Interests Dataset - Intranet Download

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service consists of location and facility identification information from EPA's Facility Registry Service (FRS) for all sites that are available in...

Basic Research Firing Facility

Data.gov (United States)

Federal Laboratory Consortium — The Basic Research Firing Facility is an indoor ballistic test facility that has recently transitioned from a customer-based facility to a dedicated basic research...

Facility effluent monitoring plan for the 300 Area Fuels Fabrication Facility

International Nuclear Information System (INIS)

Nickels, J.M.; Brendel, D.F.

1991-11-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP- 0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan is the first annual report. It shall ensure long-range integrity of the effluent monitoring system by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated as a minimum every three years. The Fuel Fabrication Facility in the Hanford 300 Area supported the production reactors from the 1940's until they were shut down in 1987. Prior to 1987 the Fuel Fabrication Facility released both airborne and liquid radioactive effluents. In January 1987 the emission of airborne radioactive effluents ceased with the shutdown of the fuels facility. The release of liquid radioactive effluents have continued although decreasing significantly from 1987 to 1990

Trauma facilities in Denmark

DEFF Research Database (Denmark)

Weile, Jesper; Nielsen, Klaus; Primdahl, Stine C

2018-01-01

Background: Trauma is a leading cause of death among adults aged challenge. Evidence supports the centralization of trauma facilities and the use multidisciplinary trauma teams. Because knowledge is sparse on the existing distribution of trauma facilities...... and the organisation of trauma care in Denmark, the aim of this study was to identify all Danish facilities that care for traumatized patients and to investigate the diversity in organization of trauma management. Methods: We conducted a systematic observational cross-sectional study. First, all hospitals in Denmark...... were identified via online services and clarifying phone calls to each facility. Second, all trauma care manuals on all facilities that receive traumatized patients were gathered. Third, anesthesiologists and orthopedic surgeons on call at all trauma facilities were contacted via telephone...

Facility Effluent Monitoring Plan for Pacific Northwest National Laboratory Balance-of-Plant Facilities

International Nuclear Information System (INIS)

Ballinger, M.Y.; Shields, K.D.

1999-01-01

The Pacific Northwest National Laboratory (PNNL) operates a number of research and development (R and D) facilities for the Department of Energy on the Hanford Site. According to DOE Order 5400.1, a Facility Effluent Monitoring Plan is required for each site, facility, or process that uses, generates, releases, or manages significant pollutants or hazardous materials. Three of the R and D facilities: the 325, 331, and 3720 Buildings, are considered major emission points for radionuclide air sampling and thus individual Facility Effluent Monitoring Plans (FEMPs) have been developed for them. Because no definition of ''significant'' is provided in DOE Order 5400.1 or the accompanying regulatory guide DOE/EH-0173T, this FEMP was developed to describe monitoring requirements in the DOE-owned, PNNL-operated facilities that do not have individual FEMPs. The remainder of the DOE-owned, PNNL-operated facilities are referred to as Balance-of-Plant (BOP) facilities. Activities in the BOP facilities range from administrative to laboratory and pilot-scale R and D. R and D activities include both radioactive and chemical waste characterization, fluid dynamics research, mechanical property testing, dosimetry research, and molecular sciences. The mission and activities for individual buildings are described in the FEMP

Facility Effluent Monitoring Plan for Pacific Northwest National Laboratory Balance-of-Plant Facilities

Energy Technology Data Exchange (ETDEWEB)

Ballinger, M.Y.; Shields, K.D.

1999-04-02

The Pacific Northwest National Laboratory (PNNL) operates a number of research and development (R and D) facilities for the Department of Energy on the Hanford Site. According to DOE Order 5400.1, a Facility Effluent Monitoring Plan is required for each site, facility, or process that uses, generates, releases, or manages significant pollutants or hazardous materials. Three of the R and D facilities: the 325, 331, and 3720 Buildings, are considered major emission points for radionuclide air sampling and thus individual Facility Effluent Monitoring Plans (FEMPs) have been developed for them. Because no definition of ''significant'' is provided in DOE Order 5400.1 or the accompanying regulatory guide DOE/EH-0173T, this FEMP was developed to describe monitoring requirements in the DOE-owned, PNNL-operated facilities that do not have individual FEMPs. The remainder of the DOE-owned, PNNL-operated facilities are referred to as Balance-of-Plant (BOP) facilities. Activities in the BOP facilities range from administrative to laboratory and pilot-scale R and D. R and D activities include both radioactive and chemical waste characterization, fluid dynamics research, mechanical property testing, dosimetry research, and molecular sciences. The mission and activities for individual buildings are described in the FEMP.

Facilities Programming.

Science.gov (United States)

Bullis, Robert V.

1992-01-01

A procedure for physical facilities management written 17 years ago is still worth following today. Each of the steps outlined for planning, organizing, directing, controlling, and evaluating must be accomplished if school facilities are to be properly planned and constructed. However, lessons have been learned about energy consumption and proper…

Facility effluent monitoring plan for the 2724-W Protective Equipment Decontamination Facility

International Nuclear Information System (INIS)

Nickels, J.M.; Lavey, G.H.

1992-12-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1* for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. A facility effluent monitoring plan determination was performed during Calendar Year 1991 and the evaluation requires the need for a facility effluent monitoring plan. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438**. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements

High Throughput Facility

Data.gov (United States)

Federal Laboratory Consortium — Argonne?s high throughput facility provides highly automated and parallel approaches to material and materials chemistry development. The facility allows scientists...

Facility effluent monitoring plan for the tank farms facilities

International Nuclear Information System (INIS)

Crummel, G.M.; Gustavson, R.D.; Kenoyer, J.L.; Moeller, M.P.

1991-11-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan is the first annual report. It shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated as a minimum three years. A variety of liquid wastes are generated in processing treatment, and disposal operations throughout the Hanford Site. The Tank Farms Project serves a major role in Hanford Site waste management activities as the temporary repository for these wastes. Stored wastes include hazardous components regulated under the Resource Conservation and Recovery Act of 1976 (RCRA) and as by-product material regulated under the Atomic Energy Act of 1954. A total of 177 single- and double-shell tanks (SST and DST) have been constructed in the 200 East and 200 West Areas of the Hanford Site. These facilities were constructed to various designs from 1943 to 1986. The Tank Farms Project is comprised of these tanks along with various transfer, receiving, and treatment facilities

Test and User Facilities | NREL

Science.gov (United States)

Test and User Facilities Test and User Facilities Our test and user facilities are available to | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z B Battery Thermal and Life Test Facility Biochemical Conversion Pilot Plant C Controllable Grid Interface Test System D Dynamometer Test Facilities

Licensed Healthcare Facilities

Data.gov (United States)

California Natural Resource Agency — The Licensed Healthcare Facilities point layer represents the locations of all healthcare facilities licensed by the State of California, Department of Health...

Facility effluent monitoring plan for the 324 Facility

International Nuclear Information System (INIS)

1994-11-01

The 324 Facility [Waste Technology Engineering Laboratory] in the 300 Area primarily supports the research and development of radioactive and nonradioactive waste vitrification technologies, biological waste remediation technologies, spent nuclear fuel studies, waste mixing and transport studies, and tritium development programs. All of the above-mentioned programs deal with, and have the potential to, release hazardous and/or radioactive material. The potential for discharge would primarily result from (1) conducting research activities using the hazardous materials, (2) storing radionuclides and hazardous chemicals, and (3) waste accumulation and storage. This report summarizes the airborne and liquid effluents, and the results of the Facility Effluent Monitoring Plan (FEMP) determination for the facility. The complete monitoring plan includes characterizing effluent streams, monitoring/sampling design criteria, a description of the monitoring systems and sample analysis, and quality assurance requirements

Cyclin-like F-box protein plays a role in growth and development of the three model species Medicago truncatula, Lotus japonicus, and Arabidopsis thaliana

Directory of Open Access Journals (Sweden)

Boycheva I

2015-08-01

Full Text Available Irina Boycheva,1 Valya Vassileva,2 Miglena Revalska,1 Grigor Zehirov,2 Anelia Iantcheva1 1Department of Functional Genetics Legumes, 2AgroBioInstitute, Department of Plant Stress Molecular Biology, Institute of Plant Physiology and Genetics, Sofia, Bulgaria Abstract: In eukaryotes, F-box proteins are one of the main components of the SCF complex that belongs to the family of ubiquitin E3 ligases, which catalyze protein ubiquitination and maintain the balance between protein synthesis and degradation. In the present study, we clarified the role and function of the gene encoding cyclin-like F-box protein from Medicago truncatula using transgenic plants of the model species M. truncatula, Lotus japonicas, and Arabidopsis thaliana generated by Agrobacterium-mediated transformation. Morphological and transcriptional analyses combined with flow cytometry and histochemistry demonstrated the participation of this protein in many aspects of plant growth and development, including processes of indirect somatic embryogenesis and symbiotic nodulation. The cyclin-like F-box gene showed expression in all plant organs and tissues comprised of actively dividing cells. The observed variations in root and hypocotyl growth, leaf and silique development, ploidy levels, and leaf parameters in the obtained transgenic lines demonstrated the effects of this gene on organ development. Furthermore, knockdown of cyclin-like F-box led to accumulation of higher levels of the G2/M transition-specific gene cyclin B1:1 (CYCB1:1, suggesting its possible role in cell cycle control. Together, the collected data suggest a similar role of the cyclin-like F-box protein in the three model species, providing evidence for the functional conservation of the studied gene. Keywords: cyclin-like F-box, model legumes, Arabidopsis thaliana, plant growth, plant development, cell cycle

Outline of NUCEF facility

International Nuclear Information System (INIS)

Takeshita, Isao

1996-01-01

NUCEF is a multipurpose research facility in the field of safety and advanced technology of nuclear fuel cycle back-end. Various experiment facilities and its supporting installations, in which nuclear fuel materials, radio isotopes and TRU elements can be handled, are arranged in more than one hundred rooms of two experiment buildings. Its construction was completed in middle of 1994 and hot experiments have been started since then. NUCEF is located on the site (30,000 m 2 ) of southeastern part in the Tokai Research Establishment of JAERI facing to the Pacific Ocean. The base of Experiment Buildings A and B was directly founded on the rock existing at 10-15 m below ground level taking the aseismatic design into consideration. Each building is almost same sized and composed of one basement and three floors of which area is 17,500 m 2 in total. In the basement, there are exhaust facilities of ventilation system, treatment system of solution fuel and radioactive waste solution and storage tanks of them. Major experiment facilities are located on the first or the second floors in each building. An air-inlet facility of ventilation system for each building is equipped on the third floor. Most of experiment facilities for criticality safety research including two critical facilities: Static Experiment Critical Facility (STACY) and Transient Experiment Critical Facility (TRACY) are installed in Experiment Building A. Experiment equipments for research on advanced fuel reprocessing process and on TRU waste management, which are named BECKY (Back End Fuel Cycle Key Elements Research Facility), are installed in laboratories and a-g cells in Experiment Building B. (J.P.N.)

Rocketball Test Facility

Data.gov (United States)

Federal Laboratory Consortium — This test facility offers the capability to emulate and measure guided missile radar cross-section without requiring flight tests of tactical missiles. This facility...

Facility Description 2012. Summary report of the encapsulation plant and disposal facility designs

International Nuclear Information System (INIS)

Palomaeki, J.; Ristimaeki, L.

2013-10-01

The purpose of the facility description is to be a specific summary report of the scope of Posiva's nuclear facilities (encapsulation plant and disposal facility) in Olkiluoto. This facility description is based on the 2012 designs and completing Posiva working reports. The facility description depicts the nuclear facilities and their operation as the disposal of spent nuclear fuel starts in Olkiluoto in about 2020. According to the decisions-in-principle of the government, the spent nuclear fuel from Loviisa and Olkiluoto nuclear power plants in operation and in future cumulative spent nuclear fuel from Loviisa 1 and 2, Olkiluoto 1, 2, 3 and 4 nuclear power plants, is permitted to be disposed of in Olkiluoto bedrock. The design of the disposal facility is based on the KBS-3V concept (vertical disposal). Long-term safety concept is based on the multi-barrier principle i.e. several release barriers, which ensure one another so that insufficiency in the performance of one barrier doesn't jeopardize long-term safety of the disposal. The release barriers are the following: canister, bentonite buffer and deposition tunnel backfill, and the host rock around the repository. The canisters are installed into the deposition holes, which are bored to the floor of the deposition tunnels. The canisters are enveloped with compacted bentonite blocks, which swell after absorbing water. The surrounding bedrock and the central and access tunnel backfill provide additional retardation, retention, and dilution. The nuclear facilities consist of an encapsulation plant and of underground final disposal facility including other aboveground buildings and surface structures serving the facility. The access tunnel and ventilation shafts to the underground disposal facility and some auxiliary rooms are constructed as a part of ONKALO underground rock characterization facility during years 2004-2014. The construction works needed for the repository start after obtaining the construction

340 Facility compliance assessment

International Nuclear Information System (INIS)

English, S.L.

1993-10-01

This study provides an environmental compliance evaluation of the RLWS and the RPS systems of the 340 Facility. The emphasis of the evaluation centers on compliance with WAC requirements for hazardous and mixed waste facilities, federal regulations, and Westinghouse Hanford Company (WHC) requirements pertinent to the operation of the 340 Facility. The 340 Facility is not covered under either an interim status Part A permit or a RCRA Part B permit. The detailed discussion of compliance deficiencies are summarized in Section 2.0. This includes items of significance that require action to ensure facility compliance with WAC, federal regulations, and WHC requirements. Outstanding issues exist for radioactive airborne effluent sampling and monitoring, radioactive liquid effluent sampling and monitoring, non-radioactive liquid effluent sampling and monitoring, less than 90 day waste storage tanks, and requirements for a permitted facility

Wastewater Treatment Facilities

Data.gov (United States)

Iowa State University GIS Support and Research Facility — Individual permits for municipal, industrial, and semi-public wastewater treatment facilities in Iowa for the National Pollutant Discharge Elimination System (NPDES)...

Energetics Conditioning Facility

Data.gov (United States)

Federal Laboratory Consortium — The Energetics Conditioning Facility is used for long term and short term aging studies of energetic materials. The facility has 10 conditioning chambers of which 2...

Facilities projects performance measurement system

International Nuclear Information System (INIS)

Erben, J.F.

1979-01-01

The two DOE-owned facilities at Hanford, the Fuels and Materials Examination Facility (FMEF), and the Fusion Materials Irradiation Test Facility (FMIT), are described. The performance measurement systems used at these two facilities are next described

Comparative metabolomics of drought acclimation in model and forage legumes.

Science.gov (United States)

Sanchez, Diego H; Schwabe, Franziska; Erban, Alexander; Udvardi, Michael K; Kopka, Joachim

2012-01-01

Water limitation has become a major concern for agriculture. Such constraints reinforce the urgent need to understand mechanisms by which plants cope with water deprivation. We used a non-targeted metabolomic approach to explore plastic systems responses to non-lethal drought in model and forage legume species of the Lotus genus. In the model legume Lotus. japonicus, increased water stress caused gradual increases of most of the soluble small molecules profiled, reflecting a global and progressive reprogramming of metabolic pathways. The comparative metabolomic approach between Lotus species revealed conserved and unique metabolic responses to drought stress. Importantly, only few drought-responsive metabolites were conserved among all species. Thus we highlight a potential impediment to translational approaches that aim to engineer traits linked to the accumulation of compatible solutes. Finally, a broad comparison of the metabolic changes elicited by drought and salt acclimation revealed partial conservation of these metabolic stress responses within each of the Lotus species, but only few salt- and drought-responsive metabolites were shared between all. The implications of these results are discussed with regard to the current insights into legume water stress physiology. © 2011 Blackwell Publishing Ltd.

Explosive Components Facility

Data.gov (United States)

Federal Laboratory Consortium — The 98,000 square foot Explosive Components Facility (ECF) is a state-of-the-art facility that provides a full-range of chemical, material, and performance analysis...

Design of the PRIDE Facility

International Nuclear Information System (INIS)

You, Gil Sung; Choung, Won Myung; Lee, Eun Pyo; Cho, Il Je; Kwon, Kie Chan; Hong, Dong Hee; Lee, Won Kyung; Ku, Jeong Hoe

2009-01-01

From 2007, KAERI is developing a PyRoprocess Integrated inactive DEmonstration facility (the PRIDE facility). The maximum annual treatment capacity of this facility will be a 10 ton-HM. The process will use a natural uranium feed material or a natural uranium mixed with some surrogate material for a simulation of a spent fuel. KAERI has also another plan to construct a demonstration facility which can treat a real spent fuel by pyroprocessing. This facility is called by ESPF, Engineering Scale Pyroprocess Facility. The ESPF will have the same treatment capability of spent fuel with the PRIDE facility. The only difference between the PRIDE and the ESPF is a radiation shielding capability. From the PRIDE facility designing works and demonstration with a simulated spent fuel after construction, it will be able to obtain the basic facility requirements, remote operability, interrelation properties between process equipment for designing of the ESPF. The flow sheet of the PRIDE processes is composed of five main processes, such as a decladding and voloxidation, an electro-reduction, an electrorefining, an electro-winning, and a salt waste treatment. The final products from the PRIDE facility are a simulated TRU metal and U metal ingot

Comprehensive facilities plan

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-09-01

The Ernest Orlando Lawrence Berkeley National Laboratory`s Comprehensive Facilities Plan (CFP) document provides analysis and policy guidance for the effective use and orderly future development of land and capital assets at the Berkeley Lab site. The CFP directly supports Berkeley Lab`s role as a multiprogram national laboratory operated by the University of California (UC) for the Department of Energy (DOE). The CFP is revised annually on Berkeley Lab`s Facilities Planning Website. Major revisions are consistent with DOE policy and review guidance. Facilities planing is motivated by the need to develop facilities for DOE programmatic needs; to maintain, replace and rehabilitate existing obsolete facilities; to identify sites for anticipated programmatic growth; and to establish a planning framework in recognition of site amenities and the surrounding community. The CFP presents a concise expression of the policy for the future physical development of the Laboratory, based upon anticipated operational needs of research programs and the environmental setting. It is a product of the ongoing planning processes and is a dynamic information source.

Conceptual design report, Sodium Storage Facility, Fast Flux Test Facility, Project F-031

International Nuclear Information System (INIS)

Shank, D.R.

1995-01-01

The Sodium Storage Facility Conceptual Design Report provides conceptual design for construction of a new facility for storage of the 260,000 gallons of sodium presently in the FFTF plant. The facility will accept the molten sodium transferred from the FFTF sodium systems, and store the sodium in a solid state under an inert cover gas until such time as a Sodium Reaction Facility is available for final disposal of the sodium

Dialysis Facility Compare

Data.gov (United States)

U.S. Department of Health & Human Services — Dialysis Facility Compare helps you find detailed information about Medicare-certified dialysis facilities. You can compare the services and the quality of care that...

Projectile Demilitarization Facilities

Data.gov (United States)

Federal Laboratory Consortium — The Projectile Wash Out Facility is US Army Ammunition Peculiar Equipment (APE 1300). It is a pilot scale wash out facility that uses high pressure water and steam...

Facility design: introduction

International Nuclear Information System (INIS)

Unger, W.E.

1980-01-01

The design of shielded chemical processing facilities for handling plutonium is discussed. The TRU facility is considered in particular; its features for minimizing the escape of process materials are listed. 20 figures

Status of RIB facilities in Asia

International Nuclear Information System (INIS)

Tanihata, Isao

1998-01-01

Radioactive Ion Beam Facilities in Asia are presented. In China, in-flight separation type facilities are in operation at the Institute of Modern Physics in Lanzhou and the other at Tandem facility in China Institute of Atomic Energy in Beijing. The storage-ring facility is proposed and approved in Lanzhou. In India, the Variable Energy Cyclotron Facility in Calcutta start to construct an ISOL-type facility. In Japan, in-flight separation type facilities are working at Research Center for Nuclear Physics in Osaka, and at RIKEN. Also a separator start its operation in medical facility in Chiba. In RIKEN, the construction of RI Beam Factory has been started. An ISOL-type facility is proposed in the Japan Hadron Facility in KEK. Table I summarize these facilities

Facility effluent monitoring plan for the plutonium-uranium extraction facility

International Nuclear Information System (INIS)

Lohrasbi, J.; Johnson, D.L.; De Lorenzo, D.S.

1993-12-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-01. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated at a minimum of every three years

Facility effluent monitoring plan for the plutonium uranium extraction facility

Energy Technology Data Exchange (ETDEWEB)

Wiegand, D.L.

1994-09-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-01. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated at a minimum of every three years.

Facility effluent monitoring plan for the plutonium uranium extraction facility

International Nuclear Information System (INIS)

Wiegand, D.L.

1994-09-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-01. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated at a minimum of every three years

Facility effluent monitoring plan for the Plutonium Uranium Extraction Facility

International Nuclear Information System (INIS)

Greager, E.M.

1997-01-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-01. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether these systems are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan will ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated, at a minimum, every 3 years

Facility effluent monitoring plan for the plutonium-uranium extraction facility

Energy Technology Data Exchange (ETDEWEB)

Lohrasbi, J.; Johnson, D.L. [Westinghouse Hanford Co., Richland, WA (United States); De Lorenzo, D.S. [Los Alamos Technical Associates, NM (United States)

1993-12-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438-01. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated at a minimum of every three years.

Integrated Disposal Facility

Data.gov (United States)

Federal Laboratory Consortium — Located near the center of the 586-square-mile Hanford Site is the Integrated Disposal Facility, also known as the IDF.This facility is a landfill similar in concept...

STAR facility tritium accountancy

International Nuclear Information System (INIS)

Pawelko, R. J.; Sharpe, J. P.; Denny, B. J.

2008-01-01

The Safety and Tritium Applied Research (STAR) facility has been established to provide a laboratory infrastructure for the fusion community to study tritium science associated with the development of safe fusion energy and other technologies. STAR is a radiological facility with an administrative total tritium inventory limit of 1.5 g (14,429 Ci) [1]. Research studies with moderate tritium quantities and various radionuclides are performed in STAR. Successful operation of the STAR facility requires the ability to receive, inventory, store, dispense tritium to experiments, and to dispose of tritiated waste while accurately monitoring the tritium inventory in the facility. This paper describes tritium accountancy in the STAR facility. A primary accountancy instrument is the tritium Storage and Assay System (SAS): a system designed to receive, assay, store, and dispense tritium to experiments. Presented are the methods used to calibrate and operate the SAS. Accountancy processes utilizing the Tritium Cleanup System (TCS), and the Stack Tritium Monitoring System (STMS) are also discussed. Also presented are the equations used to quantify the amount of tritium being received into the facility, transferred to experiments, and removed from the facility. Finally, the STAR tritium accountability database is discussed. (authors)

Synchrotron radiation facilities

CERN Multimedia

1972-01-01

Particularly in the past few years, interest in using the synchrotron radiation emanating from high energy, circular electron machines has grown considerably. In our February issue we included an article on the synchrotron radiation facility at Frascati. This month we are spreading the net wider — saying something about the properties of the radiation, listing the centres where synchrotron radiation facilities exist, adding a brief description of three of them and mentioning areas of physics in which the facilities are used.

Hierarchically Structured Electrospun Fibers

Science.gov (United States)

2013-01-07

in the natural lotus and silver ragwort leaves. Figure 4. Examples of electrospun bio-mimics of natural hierarchical structures. (A) Lotus leaf...B) pillared poly(methyl methacrylate) (PMMA) electrospun fiber mimic; (C) silver ragwort leaf; (D) electrospun fiber mimic made from nylon 6 and...domains containing the protein in the surrounding EVA fibers [115]. A wide variety of core-shell fibers have been generated, including PCL/ gelatin

The CUTLASS database facilities

International Nuclear Information System (INIS)

Jervis, P.; Rutter, P.

1988-09-01

The enhancement of the CUTLASS database management system to provide improved facilities for data handling is seen as a prerequisite to its effective use for future power station data processing and control applications. This particularly applies to the larger projects such as AGR data processing system refurbishments, and the data processing systems required for the new Coal Fired Reference Design stations. In anticipation of the need for improved data handling facilities in CUTLASS, the CEGB established a User Sub-Group in the early 1980's to define the database facilities required by users. Following the endorsement of the resulting specification and a detailed design study, the database facilities have been implemented as an integral part of the CUTLASS system. This paper provides an introduction to the range of CUTLASS Database facilities, and emphasises the role of Database as the central facility around which future Kit 1 and (particularly) Kit 6 CUTLASS based data processing and control systems will be designed and implemented. (author)

An Ornithopod-Dominated Tracksite from the Lower Cretaceous Jiaguan Formation (Barremian–Albian) of Qijiang, South-Central China: New Discoveries, Ichnotaxonomy, Preservation and Palaeoecology

Science.gov (United States)

Xing, Lida; Lockley, Martin G.; Marty, Daniel; Zhang, Jianping; Wang, Yan; Klein, Hendrik; McCrea, Richard T.; Buckley, Lisa G.; Belvedere, Matteo; Mateus, Octávio; Gierliński, Gerard D.; Piñuela, Laura; Persons, W. Scott; Wang, Fengping; Ran, Hao; Dai, Hui; Xie, Xianming

2015-01-01

The historically-famous Lotus Fortress site, a deep 1.5–3.0-meter-high, 200-meter-long horizonal notch high up in near-vertical sandstone cliffs comprising the Cretaceous Jiaguan Formation, has been known since the 13th Century as an impregnable defensive position. The site is also extraordinary for having multiple tetrapod track-bearing levels, of which the lower two form the floor of part of the notch, and yield very well preserved asseamblages of ornithopod, bird (avian theropod) and pterosaur tracks. Trackway counts indicate that ornithopods dominate (69%) accounting for at least 165 trackmakers, followed by bird (18%), sauropod (10%), and pterosaur (3%). Previous studies designated Lotus Fortress as the type locality of Caririchnium lotus and Wupus agilis both of which are recognized here as valid ichnotaxa. On the basis of multiple parallel trackways both are interpreted as representing the trackways of gregarious species. C. lotus is redescribed here in detail and interpreted to indicate two age cohorts representing subadults that were sometimes bipedal and larger quadrupedal adults. Two other previously described dinosaurian ichnospecies, are here reinterpreted as underprints and considered nomina dubia. Like a growing number of significant tetrapod tracksites in China the Lotus Fortress site reveals new information about the composition of tetrapod faunas from formations in which the skeletal record is sparse. In particular, the site shows the relatively high abundance of Caririchium in a region where saurischian ichnofaunas are often dominant. It is also the only site known to have yielded Wupus agilis. In combination with information from other tracksites from the Jiaguan formation and other Cretaceous formations in the region, the track record is proving increasingly impotant as a major source of information on the vertebrate faunas of the region. The Lotus Fortress site has been developed as a spectacular, geologically-, paleontologically- and a

An Ornithopod-Dominated Tracksite from the Lower Cretaceous Jiaguan Formation (Barremian-Albian of Qijiang, South-Central China: New Discoveries, Ichnotaxonomy, Preservation and Palaeoecology.

Directory of Open Access Journals (Sweden)

Lida Xing

Full Text Available The historically-famous Lotus Fortress site, a deep 1.5-3.0-meter-high, 200-meter-long horizonal notch high up in near-vertical sandstone cliffs comprising the Cretaceous Jiaguan Formation, has been known since the 13th Century as an impregnable defensive position. The site is also extraordinary for having multiple tetrapod track-bearing levels, of which the lower two form the floor of part of the notch, and yield very well preserved asseamblages of ornithopod, bird (avian theropod and pterosaur tracks. Trackway counts indicate that ornithopods dominate (69% accounting for at least 165 trackmakers, followed by bird (18%, sauropod (10%, and pterosaur (3%. Previous studies designated Lotus Fortress as the type locality of Caririchnium lotus and Wupus agilis both of which are recognized here as valid ichnotaxa. On the basis of multiple parallel trackways both are interpreted as representing the trackways of gregarious species. C. lotus is redescribed here in detail and interpreted to indicate two age cohorts representing subadults that were sometimes bipedal and larger quadrupedal adults. Two other previously described dinosaurian ichnospecies, are here reinterpreted as underprints and considered nomina dubia. Like a growing number of significant tetrapod tracksites in China the Lotus Fortress site reveals new information about the composition of tetrapod faunas from formations in which the skeletal record is sparse. In particular, the site shows the relatively high abundance of Caririchium in a region where saurischian ichnofaunas are often dominant. It is also the only site known to have yielded Wupus agilis. In combination with information from other tracksites from the Jiaguan formation and other Cretaceous formations in the region, the track record is proving increasingly impotant as a major source of information on the vertebrate faunas of the region. The Lotus Fortress site has been developed as a spectacular, geologically

Materiel Evaluation Facility

Data.gov (United States)

Federal Laboratory Consortium — CRREL's Materiel Evaluation Facility (MEF) is a large cold-room facility that can be set up at temperatures ranging from −20°F to 120°F with a temperature change...

Facility effluent monitoring plan for the plutonium-uranium extraction facility

International Nuclear Information System (INIS)

Thompson, R.J.; Sontage, S.

1991-11-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan is the first annual report. It shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated as a minimum every three years

AOV Facility Tool/Facility Safety Specifications -

Data.gov (United States)

Department of Transportation — Develop and maintain authorizing documents that are standards that facilities must follow. These standards are references of FAA regulations and are specific to the...

202-S Hexone Facility supplemental information to the Hanford Facility Contingency Plan

International Nuclear Information System (INIS)

Ingle, S.J.

1996-03-01

This document is a unit-specific contingency plan for the 202-S Hexone Facility and is intended to be used as a supplement to the Hanford Facility Contingency Plan. This unit-specific plan is to be used to demonstrate compliance with the contingency plan requirements of WAC 173-303 for certain Resource Conservation and Recovery Act of 1976 (RCRA) waste management units. The 202-S Hexone Facility is not used to process radioactive or nonradioactive hazardous material. Radioactive, dangerous waste material is contained in two underground storage tanks, 276-S-141 and 276-S-142. These tanks do not present a significant hazard to adjacent facilities, personnel, or the environment. Currently, dangerous waste management activities are not being applied at the tanks. It is unlikely that any incidents presenting hazards to public health or the environment would occur at the 202-S Hexone Facility

9 CFR 351.10 - Facilities.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Facilities. 351.10 Section 351.10... CERTIFICATION CERTIFICATION OF TECHNICAL ANIMAL FATS FOR EXPORT Facilities and Operations § 351.10 Facilities. (a) Facilities for the preparation, identification, and storage of the technical animal fat to be...

Greater-Than-Class C low-level radioactive waste treatment technology evaluation

International Nuclear Information System (INIS)

Garrison, T.W.; Fischer, D.K.

1993-01-01

This report was developed to provide the Greater-Than-Class C Low-Level Radioactive Waste Management Program with criteria and a methodology to select candidate treatment technologies for Greater-Than-Class C low-level radioactive waste (GTCC LLW) destined for dedicated storage and ultimately disposal. The technology selection criteria are provided in a Lotus spreadsheet format to allow the methodology to evolve as the GTCC LLW Program evolves. It is recognized that the final disposal facility is not yet defined; thus, the waste acceptance criteria and other facility-specific features are subject to change. The spreadsheet format will allow for these changes a they occur. As additional treatment information becomes available, it can be factored into the analysis. The technology selection criteria were established from program goals, draft waste acceptance criteria for dedicated storage (including applicable regulations), and accepted remedial investigation methods utilized under the Comprehensive Environmental Response, Compensation, and Liability Act. Kepner-Tregoe decisionmaking techniques are used to compare and rank technologies against the criteria

Surplus Facilities Management Program

International Nuclear Information System (INIS)

Coobs, J.H.

1983-01-01

This is the second of two programs that are concerned with the management of surplus facilities. The facilities in this program are those related to commercial activities, which include the three surplus experimental and test reactors [(MSRE, HRE-2, and the Low Intensity Test Reactor (LITR)] and seven experimental loops at the ORR. The program is an integral part of the Surplus Facilities Management Program, which is a national program administered for DOE by the Richland Operations Office. Very briefly reported here are routine surveillance and maintenance of surplus radioactively contaminated DOE facilities awaiting decommissioning

The National Ignition Facility (NIF) as a User Facility

Science.gov (United States)

Keane, Christopher; NIF Team

2013-10-01

The National Ignition Facility (NIF) has made significant progress towards operation as a user facility. Through June 2013, NIF conducted over 1200 experiments in support of ICF, HED science, and development of facility capabilities. The NIF laser has met or achieved all specifications and a wide variety of diagnostic and target fabrication capabilities are in place. A NIF User Group and associated Executive Board have been formed. Two User Group meetings have been conducted since formation of the User Group. NIF experiments in fundamental science have provided important new results. NIF ramp compression experiments have been conducted using diamond and iron, with EOS results obtained at pressures up to approximately 50 Mbar and 8 Mbar, respectively. Initial experiments in supernova hydrodynamics, the fundamental physics of the Rayleigh-Taylor instability, and equation of state in the Gbar pressure regime have also been conducted. This presentation will discuss the fundamental science program at NIF, including the proposal solicitation and scientific review processes and other aspects of user facility operation. This work was performed under the auspices of the Lawrence Livermore National Security, LLC, (LLNS) under Contract No. DE-AC52-07NA27344.

Challenges for proteomics core facilities.

Science.gov (United States)

Lilley, Kathryn S; Deery, Michael J; Gatto, Laurent

2011-03-01

Many analytical techniques have been executed by core facilities established within academic, pharmaceutical and other industrial institutions. The centralization of such facilities ensures a level of expertise and hardware which often cannot be supported by individual laboratories. The establishment of a core facility thus makes the technology available for multiple researchers in the same institution. Often, the services within the core facility are also opened out to researchers from other institutions, frequently with a fee being levied for the service provided. In the 1990s, with the onset of the age of genomics, there was an abundance of DNA analysis facilities, many of which have since disappeared from institutions and are now available through commercial sources. Ten years on, as proteomics was beginning to be utilized by many researchers, this technology found itself an ideal candidate for being placed within a core facility. We discuss what in our view are the daily challenges of proteomics core facilities. We also examine the potential unmet needs of the proteomics core facility that may also be applicable to proteomics laboratories which do not function as core facilities. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

7 CFR 58.127 - Facilities.

Science.gov (United States)

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false Facilities. 58.127 Section 58.127 Agriculture..., Facilities, Equipment and Utensils § 58.127 Facilities. (a) Water supply. There shall be an ample supply of both hot and cold water of safe and sanitary quality, with adequate facilities for its proper...

Sustainable Facilities Management

DEFF Research Database (Denmark)

Nielsen, Susanne Balslev; Elle, Morten; Hoffmann, Birgitte

2004-01-01

The Danish public housing sector has more than 20 years of experience with sustainable facilities management based on user involvement. The paper outlines this development in a historical perspective and gives an analysis of different approaches to sustainable facilities management. The focus...... is on the housing departments and strateies for the management of the use of resources. The research methods used are case studies based on interviews in addition to literature studies. The paper explores lessons to be learned about sustainable facilities management in general, and points to a need for new...

Wind Energy Facilities

Energy Technology Data Exchange (ETDEWEB)

Laurie, Carol

2017-02-01

This book takes readers inside the places where daily discoveries shape the next generation of wind power systems. Energy Department laboratory facilities span the United States and offer wind research capabilities to meet industry needs. The facilities described in this book make it possible for industry players to increase reliability, improve efficiency, and reduce the cost of wind energy -- one discovery at a time. Whether you require blade testing or resource characterization, grid integration or high-performance computing, Department of Energy laboratory facilities offer a variety of capabilities to meet your wind research needs.

Facility effluent monitoring plan for the uranium trioxide facility

International Nuclear Information System (INIS)

Thompson, R.J.; Sontag, S.

1991-11-01

A facility effluent monitoring plant is required by the US Department of Energy in DOE Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan is the first annual report. It shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated as a minimum every three years. The UO 3 Plant is located in the south-central portion of the 200 West Area of the Hanford Site. The plant consists of two primary processing buildings and several ancillary facilities. The purpose of the UO 3 Plant is to receive uranyl nitrate hexahydrate (UNH) from the Plutonium-Uranium Extraction (PUREX) Plant, concentrate it, convert the UNH to uranium trioxide (UO 3 ) powder by calcination and package it for offsite shipment. The UO 3 Plant has been placed in a standby mode. There are two liquid discharges, and three gaseous exhaust stacks, and seven building exhausters that are active during standby conditions

Nuclear physics accelerator facilities

International Nuclear Information System (INIS)

1988-12-01

This paper describes many of the nuclear physics heavy-ion accelerator facilities in the US and the research programs being conducted. The accelerators described are: Argonne National Laboratory--ATLAS; Brookhaven National Laboratory--Tandem/AGS Heavy Ion Facility; Brookhaven National Laboratory--Relativistic Heavy Ion Collider (RHIC) (Proposed); Continuous Electron Beam Accelerator Facility; Lawrence Berkeley Laboratory--Bevalac; Lawrence Berkeley Laboratory--88-Inch Cyclotron; Los Alamos National Laboratory--Clinton P. Anderson Meson Physics Facility (LAMPF); Massachusetts Institute of Technology--Bates Linear Accelerator Center; Oak Ridge National Laboratory--Holifield Heavy Ion Research Facility; Oak Ridge National Laboratory--Oak Ridge Electron Linear Accelerator; Stanford Linear Accelerator Center--Nuclear Physics Injector; Texas AandM University--Texas AandM Cyclotron; Triangle Universities Nuclear Laboratory (TUNL); University of Washington--Tandem/Superconducting Booster; and Yale University--Tandem Van de Graaff

National Ignition Facility system design requirements conventional facilities SDR001

International Nuclear Information System (INIS)

Hands, J.

1996-01-01

This System Design Requirements (SDR) document specifies the functions to be performed and the minimum design requirements for the National Ignition Facility (NIF) site infrastructure and conventional facilities. These consist of the physical site and buildings necessary to house the laser, target chamber, target preparation areas, optics support and ancillary functions

Irradiation facilities in JRR-3M

International Nuclear Information System (INIS)

Ohtomo, Akitoshi; Sigemoto, Masamitsu; Takahashi, Hidetake

1992-01-01

Irradiation facilities have been installed in the upgraded JRR-3 (JRR-3M) in Japan Atomic Energy Research Institute (JAERI). There are hydraulic rabbit facilities (HR), pneumatic rabbit facilities (PN), neutron activation analysis facility (PN3), uniform irradiation facility (SI), rotating irradiation facility and capsule irradiation facilities to carry out the neutron irradiation in the JRR-3M. These facilities are operated using a process control computer system to centerize the process information. Some of the characteristics for the facilities were satisfactorily measured at the same time of reactor performance test in 1990. During reactor operation, some of the tests are continued to confirm the basic characteristics on facilities, for example, PN3 was confirmed to have enough performance for activation analysis. Measurement of neutron flux at all irradiation positions has been carried out for the equilibrium core. (author)

Facility effluent monitoring plan for the tank farms facilities

Energy Technology Data Exchange (ETDEWEB)

Bachand, D.D.; Crummel, G.M.

1995-05-01

A facility effluent monitoring plan is required by the US Department of Energy for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using specific guidelines. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated as a minimum every three years.

21 CFR 606.40 - Facilities.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Facilities. 606.40 Section 606.40 Food and Drugs... GOOD MANUFACTURING PRACTICE FOR BLOOD AND BLOOD COMPONENTS Plant and Facilities § 606.40 Facilities. Facilities shall be maintained in a clean and orderly manner, and shall be of suitable size, construction and...

Hanford Surplus Facilities Program plan

International Nuclear Information System (INIS)

Hughes, M.C.; Wahlen, R.K.; Winship, R.A.

1989-09-01

The Hanford Surplus Facilities Program is responsible for the safe and cost-effective surveillance, maintenance, and decommissioning of surplus facilities at the Hanford Site. The management of these facilities requires a surveillance and maintenance program to keep them in a safe condition and development of a plan for ultimate disposition. Criteria used to evaluate each factor relative to decommissioning are based on the guidelines presented by the US Department of Energy-Richland Operations Office, Defense Facilities Decommissioning Program Office, and are consistent with the Westinghouse Hanford Company commitment to decommission the Hanford Site retired facilities in the safest and most cost-effective way achievable. This document outlines the plan for managing these facilities to the end of disposition

Green facility location

NARCIS (Netherlands)

Velázquez Martínez, J.C.; Fransoo, J.C.; Bouchery, Y.; Corbett, C.J.; Fransoo, J.C.; Tan, T.

2017-01-01

Transportation is one of the main contributing factors of global carbon emissions, and thus, when dealing with facility location models in a distribution context, transportation emissions may be substantially higher than the emissions due to production or storage. Because facility location models

Tritium breeding measurements in a lithium blanket module with Pb/Be multipliers at the LOTUS facility

International Nuclear Information System (INIS)

Azam, S.; Kumar, A.

1987-01-01

The lithium blanket module (LBM) was lent for a fixed duration in 1985 to Ecole Polytechnique Federale de Lausanne under an agreement with the Electric Power Research Institute and Princeton Plasma Physics Laboratory. The first tritium breeding measurements in the central rod of the LBM and their analysis have been reported previously. Some time ago, we carried out additional experiments wherein the Li 2 O sample disk, each having a theoretical density of ∼85% and dimensions of 17.8-mm diam x 0.9-mm thickness, were placed in four removable rods. In addition to the central rod, the other rods were at ∼6-, 18-, and 39-cm radial distances from the axis of the central one. The sample disks wee kept at every 3 cm inside each of these rods up to a length of 30 cm in the Li 2 O part of the LBM. The choice of the off-axis rods resulted from our interest in investigating the effect of room return on tritium breeding in the LBM. We chose two of the leading neutron multipliers: (a) a 5-cm-thick (∼100- x 110-cm) lead slab and (b) a 6-cm-thick (∼66- x 66-cm) beryllium slab. The experimental assembly, consisting of the multiplier followed by the LBM, was kept at 10 cm from the generator. A packet of three foils, zirconium, indium, and aluminum, was placed at the center of the flat face of the generator to monitor the source intensity during the 10-h operation for the experiments with each multiplier. The source intensity is deduced to be ∼1.9 x 10 12 n/s for both the experiments. 5 refs., 3 figs

Requirements of on-site facilities

International Nuclear Information System (INIS)

Burchardt, H.

1977-01-01

1) Requirements of on-site facilities: a) brief description of supplying the site with electricity and water; communication facilities, b) necessary facilities for containment and pipeline installation, c) necessary facilities for storage, safety, accommodation of personnel, housing; workshops; 2) Site management: a) Organisation schedules for 'turn-key-jobs' and 'single commission', b) Duties of the supervisory staff. (orig.) [de

304 Concretion facility closure plan

International Nuclear Information System (INIS)

1990-04-01

The Hanford Site, located northwest of Richland, Washington, houses reactors, chemical-separation systems, and related facilities used for the production of special nuclear materials. The 300 Area of the Hanford Site contains reactor fuel manufacturing facilities and several research and development laboratories. Recyclable scrap uranium Zircaloy-2 and copper silicon alloy, uranium-titanium alloy, beryllium/Zircaloy-2 alloy, and Zircaloy-2 chips and fines were secured in concrete billets in the 304 Concretion Facility, located in the 300 Area. The beryllium/Zircaloy-2 alloy and Zircaloy-2 chips and fines are designated as low-level radioactive mixed waste (LLRMW) with the characteristic of ignitability. The concretion process reduced the ignitability of the fines and chips for safe storage and shipment. This process has been discontinued and the 304 Concretion Facility is now undergoing closure as defined in the Resource Conservation and Recovery Act of 1976 (RCRA). This closure plan presents a description of the facility, the history of materials and wastes managed, and the procedures that will be followed to close the 304 Concretion Facility (304 Facility). Clean closure of the 304 Facility is the proposed method for closure of the facility. Justification for this proposal is presented. 15 refs., 22 figs., 4 tabs

Decommissioning nuclear facilities

International Nuclear Information System (INIS)

Harmon, K.M.; Jenkins, C.E.; Waite, D.A.; Brooksbank, R.E.; Lunis, B.C.; Nemec, J.F.

1976-01-01

This paper describes the currently accepted alternatives for decommissioning retired light water reactor fuel cycle facilities and the current state of decommissioning technology. Three alternatives are recognized: Protective Storage; Entombment; and Dismantling. Application of these alternatives to the following types of facilities is briefly described: light water reactors; fuel reprocessing plants, and mixed oxide fuel fabrication plants. Brief descriptions are given of decommissioning operations and results at a number of sites, and recent studies of the future decommissioning of prototype fuel cycle facilities are reviewed. An overview is provided of the types of operations performed and tools used in common decontamination and decommissioning techniques and needs for improved technology are suggested. Planning for decommissioning a nuclear facility is dependent upon the maximum permitted levels of residual radioactive contamination. Proposed guides and recently developed methodology for development of site release criteria are reviewed. 21 fig, 32 references

Lotus Effect Toy

Science.gov (United States)

Shakerin, Said

2011-01-01

This is a short article about Aqua Drop, a toy that employs a superhydrophobic surface. After defining superhydrophobic surface, its occurrence in nature, and its importance in science and technology, I describe the toy, followed by several student activities that can be performed with the toy and easily found natural objects.

National Solar Thermal Test Facility

Data.gov (United States)

Federal Laboratory Consortium — The National Solar Thermal Test Facility (NSTTF) is the only test facility in the United States of its type. This unique facility provides experimental engineering...

Associations between Moderate-to-Vigorous Physical Activity and Neighbourhood Recreational Facilities: The Features of the Facilities Matter

Directory of Open Access Journals (Sweden)

Ka Yiu Lee

2014-12-01

Full Text Available Objectives: To examine the associations between objectively-assessed moderate-to-vigorous physical activity (MVPA and perceived/objective measures of neighbourhood recreational facilities categorized into indoor or outdoor, public, residential or commercial facilities. The associations between facility perceptions and objectively-assessed numbers of recreational facilities were also examined. Method: A questionnaire was used on 480 adults to measure local facility perceptions, with 154 participants wearing ActiGraph accelerometers for ≥4 days. The objectively-assessed number of neighbourhood recreational facilities were examined using direct observations and Geographical Information System data. Results: Both positive and negative associations were found between MVPA and perceived/objective measures of recreational facilities. Some associations depended on whether the recreational facilities were indoor or outdoor, public or residential facilities. The objectively-assessed number of most public recreational facilities was associated with the corresponding facility perceptions, but the size of effect was generally lower than for residential recreational facilities. Conclusions: The objectively-assessed number of residential outdoor table tennis courts and public indoor swimming pools, the objectively-assessed presence of tennis courts and swimming pools, and the perceived presence of bike lanes and swimming pools were positive determinants of MVPA. It is suggested to categorize the recreational facilities into smaller divisions in order to identify unique associations with MVPA.

WORKSHOPS: Hadron facilities

International Nuclear Information System (INIS)

Anon.

1987-01-01

'Hadron facilities' – high intensity (typically a hundred microamps), medium energy (30-60 GeV) machines producing intense secondary beams of pions, kaons, etc., are being widely touted as a profitable research avenue to supplement what is learned through the thrust for higher and higher energies. This interest was reflected at an International Workshop on Hadron Facility Technology, held in Santa Fe, New Mexico. As well as invited talks describing the various projects being pushed in the US, Europe and Japan, the meeting included working groups covering linacs, beam dynamics, hardware, radiofrequency, polarized beams and experimental facilities

Investigating walking environments in and around assisted living facilities: a facility visit study.

Science.gov (United States)

Lu, Zhipeng

2010-01-01

This study explores assisted living residents' walking behaviors, locations where residents prefer to walk, and walking environments in and around assisted living facilities. Regular walking is beneficial to older adults' physical and psychological health. Yet frail older residents in assisted living are usually too sedentary to achieve these benefits. The physical environment plays an important role in promoting physical activity. However, there is little research exploring this relationship in assisted living settings. The researcher visited 34 assisted living facilities in a major Texas city. Methods included walk-through observation with the Assisted Living Facility Walking Environment Checklist, and interviews with administrators by open- and close-ended questions. The data from 26 facilities were analyzed using descriptive statistics (for quantitative data) and content analysis (for qualitative data). The results indicate that (a) residents were walking both indoors and outdoors for exercise or other purposes (e.g., going to destinations); (b) assisted living facility planning and design details-such as neighborhood sidewalk conditions, facility site selection, availability of seating, walking path configuration (e.g., looped/nonlooped path), amount of shading along the path, presence of handrails, existence of signage, etc.-may influence residents' walking behaviors; and (c) current assisted living facilities need improvement in all aspects to make their environments more walkable for residents. Findings of the study provide recommendations for assisted living facilities to improve the walkability of environments and to create environmental interventions to promote regular walking among their residents. This study also implies several directions for future research.

Waste isolation facility description: bedded salt

Energy Technology Data Exchange (ETDEWEB)

1976-09-01

The waste isolation facility is designed to receive and store three basic types of solidified wastes: high-level wastes, intermediate level high-gamma transuranic waste, and low-gamma transuranic wastes. The facility under consideration in this report is designed for bedded salt at a depth of approximately 1800 ft. The present design for the facility includes an area which would be used initially as a pilot facility to test the viability of the concept, and a larger facility which would constitute the final storage area. The total storage area in the pilot facility is planned to be 77 acres and in the fuel facility 1601 acres. Other areas for shaft operations and access would raise the overall size of the total facility to slightly less than 2,000 acres. The following subjects are discussed in detail: surface facilities, shaft design and characteristics, design and construction of the underground waste isolation facility, ventilation systems, and design requirements and criteria. (LK)

Waste isolation facility description: bedded salt

International Nuclear Information System (INIS)

1976-09-01

The waste isolation facility is designed to receive and store three basic types of solidified wastes: high-level wastes, intermediate level high-gamma transuranic waste, and low-gamma transuranic wastes. The facility under consideration in this report is designed for bedded salt at a depth of approximately 1800 ft. The present design for the facility includes an area which would be used initially as a pilot facility to test the viability of the concept, and a larger facility which would constitute the final storage area. The total storage area in the pilot facility is planned to be 77 acres and in the fuel facility 1601 acres. Other areas for shaft operations and access would raise the overall size of the total facility to slightly less than 2,000 acres. The following subjects are discussed in detail: surface facilities, shaft design and characteristics, design and construction of the underground waste isolation facility, ventilation systems, and design requirements and criteria

Investigation on candidates of principal facilities for exposure dose to public for the facilities using nuclear material

International Nuclear Information System (INIS)

Shimazaki, Yosuke; Sawahata, Hiroaki; Takada, Shoji; Fujimoto, Nozomu

2015-01-01

HTTR holds the nuclear fuel material use facilities in its reactor facilities, for the purpose of study on the fracture behavior of fuel and release behavior of fission products, development of high-performance fuel, and measurement of neutron flux. Due to the revision of the 'Act on the regulation of nuclear source material, nuclear fuel material and reactor', the facilities having the 'Important safety-related facilities' among the facilities applicable to the Enforcement Ordinance Article 41 (Article 41 facilities) has come to need to conform to the 'Regulations concerning standards for the location, structure, and equipment of used facilities and others'. In this case, actions such as modification by all possible means are required. The nuclear fuel substance use facilities of HTTR correspond to Article 41 facilities. So, whether it is a candidate for the 'Important safety-related facilities' has been examined. As a result, it is confirmed that the facilities are not correspond to the 'Important safety-related facilities', and it has been concluded that modification measures for the purpose of conforming to this approval standard rule are not necessary as of the present. (A.O.)

10 CFR 611.206 - Existing facilities.

Science.gov (United States)

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Existing facilities. 611.206 Section 611.206 Energy... PROGRAM Facility/Funding Awards § 611.206 Existing facilities. The Secretary shall, in making awards to those manufacturers that have existing facilities, give priority to those facilities that are oldest or...

33 CFR 154.1216 - Facility classification.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Facility classification. 154.1216... Vegetable Oils Facilities § 154.1216 Facility classification. (a) The Coast Guard classifies facilities that... classification of a facility that handles, stores, or transports animal fats or vegetable oils. The COTP may...

Mound facility physical characterization

Energy Technology Data Exchange (ETDEWEB)

Tonne, W.R.; Alexander, B.M.; Cage, M.R.; Hase, E.H.; Schmidt, M.J.; Schneider, J.E.; Slusher, W.; Todd, J.E.

1993-12-01

The purpose of this report is to provide a baseline physical characterization of Mound`s facilities as of September 1993. The baseline characterizations are to be used in the development of long-term future use strategy development for the Mound site. This document describes the current missions and alternative future use scenarios for each building. Current mission descriptions cover facility capabilities, physical resources required to support operations, current safety envelope and current status of facilities. Future use scenarios identify potential alternative future uses, facility modifications required for likely use, facility modifications of other uses, changes to safety envelope for the likely use, cleanup criteria for each future use scenario, and disposition of surplus equipment. This Introductory Chapter includes an Executive Summary that contains narrative on the Functional Unit Material Condition, Current Facility Status, Listing of Buildings, Space Plans, Summary of Maintenance Program and Repair Backlog, Environmental Restoration, and Decontamination and Decommissioning Programs. Under Section B, Site Description, is a brief listing of the Site PS Development, as well as Current Utility Sources. Section C contains Site Assumptions. A Maintenance Program Overview, as well as Current Deficiencies, is contained within the Maintenance Program Chapter.

18 CFR 1317.410 - Comparable facilities.

Science.gov (United States)

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Comparable facilities... facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities provided for students of one sex shall be comparable to such facilities provided...

Service quality in contracted facilities.

Science.gov (United States)

Rabbani, Fauziah; Pradhan, Nousheen Akber; Zaidi, Shehla; Azam, Syed Iqbal; Yousuf, Farheen

2015-01-01

The purpose of this paper is to explore the readiness of contracted and non-contracted first-level healthcare facilities in Pakistan to deliver quality maternal and neonatal health (MNH) care. A balanced scorecard (BSC) was used as the assessment framework. Using a cross-sectional study design, two rural health centers (RHCs) contracted out to Aga Khan Health Service, Pakistan were compared with four government managed RHCs. A BSC was designed to assess RHC readiness to deliver good quality MNH care. In total 20 indicators were developed, representing five BSC domains: health facility functionality, service provision, staff capacity, staff and patient satisfaction. Validated data collection tools were used to collect information. Pearson χ2, Fisher's Exact and the Mann-Whitney tests were applied as appropriate to detect significant service quality differences among the two facilities. Contracted facilities were generally found to be better than non-contracted facilities in all five BSC domains. Patients' inclination for facility-based delivery at contracted facilities was, however, significantly higher than non-contracted facilities (80 percent contracted vs 43 percent non-contracted, p=0.006). The study shows that contracting out initiatives have the potential to improve MNH care. This is the first study to compare MNH service delivery quality across contracted and non-contracted facilities using BSC as the assessment framework.

Incidence and predictors of permanent pacemaker implantation following treatment with the repositionable Lotus™ transcatheter aortic valve.

Science.gov (United States)

Zaman, Sarah; McCormick, Liam; Gooley, Robert; Rashid, Hashrul; Ramkumar, Satish; Jackson, Damon; Hui, Samuel; Meredith, Ian T

2017-07-01

To determine the incidence and predictors of permanent pacemaker (PPM) requirement following transcatheter aortic valve replacement (TAVR) with the mechanically expanded Lotus TM Valve System (Boston Scientific). Pacemaker implantation is the most common complication following TAVR. Predictors of pacing following TAVR with the Lotus valve have not been systematically assessed. Consecutive patients with severe aortic stenosis who underwent Lotus valve implantation were prospectively recruited at a single-centre. Patients with a pre-existing PPM were excluded. Baseline ECG, echocardiographic and multiple detector computed tomography as well as procedural telemetry and depth of implantation were independently analyzed in a blinded manner. The primary endpoint was 30-day incidence of pacemaker requirement (PPM implantation or death while pacing-dependent). Multivariate analysis was performed to identify independent predictors of the primary endpoint. A total of 104 consecutive patients underwent TAVR with the Lotus valve with 9/104 (9%) with a pre-existing PPM excluded. New or worsened procedural LBBB occurred in 78%. Thirty-day incidence of the primary pacing endpoint was 28%. The most common indication for PPM implantation was complete heart block (CHB) (69%). Independent predictors of the primary endpoint included pre-existing RBBB (hazard ratio [HR] 2.8, 95% CI 1.1-7.0; P = 0.032) and depth of implantation below the noncoronary cusp (NCC) (HR 2.4, 95% CI 1.0-5.7; P = 0.045). Almost a third of Lotus valve recipients require pacemaker implantation within 30 days. The presence of pre-existing RBBB and the depth of prosthesis implantation below the NCC were significant pacing predictors. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Facility of aerosol filtration

Energy Technology Data Exchange (ETDEWEB)

Duverger de Cuy, G; Regnier, J

1975-04-18

Said invention relates to a facility of aerosol filtration, particularly of sodium aerosols. Said facility is of special interest for fast reactors where sodium fires involve the possibility of high concentrations of sodium aerosols which soon clog up conventional filters. The facility intended for continuous operation, includes at the pre-filtering stage, means for increasing the size of the aerosol particles and separating clustered particles (cyclone separator).

75 FR 55574 - Joint Public Roundtable on Swap Execution Facilities and Security-Based Swap Execution Facilities

Science.gov (United States)

2010-09-13

...; File No. 4-612] Joint Public Roundtable on Swap Execution Facilities and Security-Based Swap Execution Facilities AGENCY: Commodity Futures Trading Commission (``CFTC'') and Securities and Exchange Commission... discuss swap execution facilities and security-based swap execution facilities in the context of certain...

9 CFR 3.27 - Facilities, outdoor.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Facilities, outdoor. 3.27 Section 3.27... Pigs and Hamsters Facilities and Operating Standards § 3.27 Facilities, outdoor. (a) Hamsters shall not be housed in outdoor facilities. (b) Guinea pigs shall not be housed in outdoor facilities unless...

Global Environment Facility |

Science.gov (United States)

environment Countries pledge US$4.1 billion to the Global Environment Facility Ringtail lemur mom with two of paradise Nations rally to protect global environment Countries pledge US$4.1 billion to the Global Environment Facility Stockholm, Sweden birds-eye view Events GEF-7 Replenishment Trung Truong Son Landscapes

Realities of proximity facility siting

International Nuclear Information System (INIS)

DeMott, D.L.

1981-01-01

Numerous commercial nuclear power plant sites have 2 to 3 reactors located together, and a group of Facilities with capabilities for fuel fabrication, a nuclear reactor, a storage area for spent fuel, and a maintenance area for contaminated equipment and radioactive waste storage are being designed and constructed in the US. The proximity of these facilities to each other provides that the ordinary flow of materials remain within a limited area. Interactions between the various facilities include shared resources such as communication, fire protection, security, medical services, transportation, water, electrical, personnel, emergency planning, transport of hazardous material between facilities, and common safety and radiological requirements between facilities. This paper will explore the advantages and disadvantages of multiple facilities at one site. Problem areas are identified, and recommendations for planning and coordination are discussed

LOTUS— Preparing Sentinel-3 SAR Altimetry Processing for Ocean and Land

DEFF Research Database (Denmark)

Knudsen, Per; Andersen, Ole Baltazar; Nielsen, Karina

2016-01-01

methods and processing chains need to be developed. Subsequently, new potential Copernicus products should be developed that utilize the improved alongtrack resolution over both the oceans and over land. The main objective of the LOTUS project is to prepare the scientific and operational use of data from......The Sentinel-3 satellite mission with its SRAL instrumentation contains new features compared to the conventional radar altimeter mission that form the basis for new innovative scientific analyses of both ocean and inland water levels. To utilize the full potential of the new data source, new...... that they will be used for commercial activities. LOTUS will develop processing scheme for extracting high-resolution sea surface heights, wave heights and wind speeds from SAR mode data. Over land, the LOTUS will develop processing scheme for extracting high-resolution river and lake heights, soil moisture, and snow...

LOTUS— Preparing Sentinel-3 Ocean and Land SAR Altimetry Processing for Copernicus

DEFF Research Database (Denmark)

Knudsen, Per; Andersen, Ole Baltazar; Jain, Maulik

2014-01-01

for commercial activities. The main objectives of the LOTUS project is to prepare the take-up of data from Sentinels 3. In the initial phase, LOTUS will develop processing scheme for extracting high-resolution sea surface heights, wave heights and wind speeds from SAR mode data. Over land, the LOTUS will develop...... potential of the new data source, new methods and processing chains need to be developed. Also, new potential Copernicus products should be developed that utilize the improved along-track resolution over both the oceans and over land. Then new operational processing, validation and delivery mechanisms need...... processing scheme for extracting high-resolution river and lake heights, soil moisture, and snow water equivalents. This presentation show some preliminary results based on analyses using CRYOSAT data. Furthermore, new DEMO data sets are presented. These data sets facilitate the development of marine...

Radiological Research Accelerator Facility

International Nuclear Information System (INIS)

Goldhagen, P.; Marino, S.A.; Randers-Pehrson, G.; Hall, E.J.

1986-01-01

The Radiological Research Accelerator Facility (RARAF) is based on a 4-MV Van de Graaff accelerator, which can be used to generate a variety of well-characterized radiation beams for research in radiobiology and radiological physics. It is part of the Radiological Research Laboratory (RRL), and its operation is supported as a National Facility by the US Department of Energy. RARAF is available to all potential users on an equal basis, with priorities based on the recommendations of a Scientific Advisory Committee. Facilities and services are provided to users, but the research projects themselves must be supported separately. This chapter presents a brief description of current experiments being carried out at RARAF and of the operation of the Facility from January through June, 1986. Operation of the Facility for all of 1985 was described in the 1985 Progress Report for RARAF. The experiments described here were supported by various Grants and Contracts from NIH and DOE and by the Statens Stralskyddsinstitut of Sweden

Active use of urban park facilities

DEFF Research Database (Denmark)

Lindberg, Michael; Schipperijn, Jasper

2015-01-01

groups of the facilities. Facilities that provide the opportunity for games and playing activities are more used for PA than facilities directed at individual training of strength and fitness. The opportunity for socializing and playing while being physically active seems important for many users...... was mentioned as a key factor when designing facilities. Our results provide important knowledge to architects, planners and policy makers when aiming at designing activity-promoting facilities in UGS. Future studies need to further investigate the use of facilities among specific target groups, particularly......Abstract Urban green spaces (UGS), and more specific a higher number of facilities in UGS, have been positively associated with physical activity (PA). However, more detailed studies of which facilities generate high levels of PA, for which type of users, are relevant as existing knowledge...

Environmental Toxicology Research Facility

Data.gov (United States)

Federal Laboratory Consortium — Fully-equipped facilities for environmental toxicology researchThe Environmental Toxicology Research Facility (ETRF) located in Vicksburg, MS provides over 8,200 ft...

304 Concretion Facility Closure Plan

International Nuclear Information System (INIS)

1991-10-01

The Hanford Site, located northwest of Richland, Washington, houses reactors, chemical-separation systems, and related facilities used for the production of special nuclear materials. The 300 Area of the Hanford Site contains reactor fuel manufacturing facilities and several research and development laboratories. Recyclable scrap uranium with Zircaloy-2 and copper silicon allo , uranium-titanium alloy, beryllium/Zircaloy-2 alloy, and Zircaloy-2 chips and fines were secured in concrete billets (7.5-gal containers) in the 304 Concretion Facility (304 Facility), located in the 300 Area. The beryllium/Zircaloy-2 alloy and Zircaloy-2 chips and fines are designated as low-level radioactive mixed waste (LLRMW) with the characteristic of ignitability. The concretion process reduced the ignitability of the fines and chips for safe storage and shipment. This process has been discontinued and the 304 Concretion Facility is now undergoing closure as defined in the Resource Conservation and Recovery Act of 1976 (RCRA) and the Washington Administrative Code (WAC) Dangerous Waste Regulations, WAC 173-303-040 (Ecology 1991). This closure plan presents a description of the facility, the history of materials and wastes managed, and the procedures that will be followed to close the 304 Facility. The strategy for closure of the 304 Facility is presented in Section 6.0

ORNL Isotopes Facilities Shutdown Program Plan

International Nuclear Information System (INIS)

Gibson, S.M.; Patton, B.D.; Sears, M.B.

1990-10-01

This plan presents the results of a technical and economic assessment for shutdown of the Oak Ridge National Laboratory (ORNL) isotopes production and distribution facilities. On December 11, 1989, the Department of Energy (DOE), Headquarters, in a memorandum addressed to DOE Oak Ridge Operations Office (DOE-ORO), gave instructions to prepare the ORNL isotopes production and distribution facilities, with the exception of immediate facility needs for krypton-85, tritium, and yttrium-90, for safe shutdown. In response to the memorandum, ORNL identified 17 facilities for shutdown. Each of these facilities is located within the ORNL complex with the exception of Building 9204-3, which is located at the Y-12 Weapons Production Plant. These facilities have been used extensively for the production of radioactive materials by the DOE Isotopes Program. They currently house a large inventory of radioactive materials. Over the years, these aging facilities have inherited the problems associated with storing and processing highly radioactive materials (i.e., facilities' materials degradation and contamination). During FY 1990, ORNL is addressing the requirements for placing these facilities into safe shutdown while maintaining the facilities under the existing maintenance and surveillance plan. The day-to-day operations associated with the surveillance and maintenance of a facility include building checks to ensure that building parameters are meeting the required operational safety requirements, performance of contamination control measures, and preventative maintenance on the facility and facility equipment. Shutdown implementation will begin in FY 1993, and shutdown completion will occur by the end of FY 1994

Aperture area measurement facility

Data.gov (United States)

Federal Laboratory Consortium — NIST has established an absolute aperture area measurement facility for circular and near-circular apertures use in radiometric instruments. The facility consists of...

Facility Registry Service (FRS)

Data.gov (United States)

U.S. Environmental Protection Agency — The Facility Registry Service (FRS) provides an integrated source of comprehensive (air, water, and waste) environmental information about facilities across EPA,...

Fast Flux Test Facility, Sodium Storage Facility project-specific project management plan

International Nuclear Information System (INIS)

Shank, D.R.

1994-01-01

This Project-Specific Project Management Plan describes the project management methods and controls used by the WHC Projects Department to manage Project 03-F-031. The Sodium Storage Facility provides for storage of the 260,000 gallons of sodium presently in the FFTF Plant. The facility will accept the molten sodium transferred from the FFTF sodium systems, and store the sodium in a solid state under an inert cover gas until such time as a Sodium Reaction Facility is available for final disposal of the sodium

Fast Flux Test Facility, Sodium Storage Facility project-specific project management plan

Energy Technology Data Exchange (ETDEWEB)

Shank, D.R.

1994-12-29

This Project-Specific Project Management Plan describes the project management methods and controls used by the WHC Projects Department to manage Project 03-F-031. The Sodium Storage Facility provides for storage of the 260,000 gallons of sodium presently in the FFTF Plant. The facility will accept the molten sodium transferred from the FFTF sodium systems, and store the sodium in a solid state under an inert cover gas until such time as a Sodium Reaction Facility is available for final disposal of the sodium.

JRR-3 neutron radiography facility

International Nuclear Information System (INIS)

Matsubayashi, M.; Tsuruno, A.

1992-01-01

JRR-3 neutron radiography facility consists of thermal neutron radiography facility (TNRF) and cold neutron radiography facility (CNRF). TNRF is installed in JRR-3 reactor building. CNRF is installed in the experimental beam hall adjacent to the reactor building. (author)

Armament Technology Facility (ATF)

Data.gov (United States)

Federal Laboratory Consortium — The Armament Technology Facility is a 52,000 square foot, secure and environmentally-safe, integrated small arms and cannon caliber design and evaluation facility....

Simulation of facility operations and materials accounting for a combined reprocessing/MOX fuel fabrication facility

International Nuclear Information System (INIS)

Coulter, C.A.; Whiteson, R.; Zardecki, A.

1991-01-01

We are developing a computer model of facility operations and nuclear materials accounting for a facility that reprocesses spent fuel and fabricates mixed oxide (MOX) fuel rods and assemblies from the recovered uranium and plutonium. The model will be used to determine the effectiveness of various materials measurement strategies for the facility and, ultimately, of other facility safeguards functions as well. This portion of the facility consists of a spent fuel storage pond, fuel shear, dissolver, clarifier, three solvent-extraction stages with uranium-plutonium separation after the first stage, and product concentrators. In this facility area mixed oxide is formed into pellets, the pellets are loaded into fuel rods, and the fuel rods are fabricated into fuel assemblies. These two facility sections are connected by a MOX conversion line in which the uranium and plutonium solutions from reprocessing are converted to mixed oxide. The model of the intermediate MOX conversion line used in the model is based on a design provided by Mike Ehinger of Oak Ridge National Laboratory (private communication). An initial version of the simulation model has been developed for the entire MOX conversion and fuel fabrication sections of the reprocessing/MOX fuel fabrication facility, and this model has been used to obtain inventory difference variance estimates for those sections of the facility. A significant fraction of the data files for the fuel reprocessing section have been developed, but these data files are not yet complete enough to permit simulation of reprocessing operations in the facility. Accordingly, the discussion in the following sections is restricted to the MOX conversion and fuel fabrication lines. 3 tabs

Lesotho - Health Facility Survey

Data.gov (United States)

Millennium Challenge Corporation — The main objective of the 2011 Health Facility Survey (HFS) was to establish a baseline for informing the Health Project performance indicators on health facilities,...

20 CFR 416.1023 - Facilities.

Science.gov (United States)

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Facilities. 416.1023 Section 416.1023... Facilities. (a) Space, equipment, supplies, and other services. Subject to appropriate Federal funding, the... and prompt disability determinations. (b) Location of facilities. Subject to appropriate Federal...

Procedure for estimating facility decommissioning costs for non-fuel-cycle nuclear facilities

International Nuclear Information System (INIS)

Short, S.M.

1988-01-01

The Nuclear Regulatory Commission (NRC) staff has been reappraising its regulatory position relative to the decommissioning of nuclear facilities over the last several years. Approximately 30 reports covering the technology, safety, and costs of decommissioning reference nuclear facilities have been published during this period in support of this effort. One of these reports, Technology, Safety, and Costs of Decommissioning Reference Non-Fuel-Cycle Nuclear Facilities (NUREG/CR-1754), was published in 1981 and was felt by the NRC staff to be outdated. The Pacific Northwest Laboratory (PNL) was asked by the NRC staff to revise the information provided in this report to reflect the latest information on decommissioning technology and costs and publish the results as an addendum to the previous report. During the course of this study, the NRC staff also asked that PNL provide a simplified procedure for estimating decommissioning costs of non-fuel-cycle nuclear facilities. The purpose being to provide NRC staff with the means to easily generate their own estimate of decommissioning costs for a given facility for comparison against a licensee's submittal. This report presents the procedure developed for use by NRC staff

PUREX facility preclosure work plan

International Nuclear Information System (INIS)

Engelmann, R.H.

1997-01-01

This preclosure work plan presents a description of the PUREX Facility, the history of the waste managed, and addresses transition phase activities that position the PUREX Facility into a safe and environmentally secure configuration. For purposes of this documentation, the PUREX Facility does not include the PUREX Storage Tunnels (DOE/RL-90/24). Information concerning solid waste management units is discussed in the Hanford Facility Dangerous Waste Permit Application, General Information Portion (DOE/RL-91-28, Appendix 2D)

Facilities for US Radioastronomy.

Science.gov (United States)

Thaddeus, Patrick

1982-01-01

Discusses major developments in radioastronomy since 1945. Topics include proposed facilities, very-long-baseline interferometric array, millimeter-wave telescope, submillimeter-wave telescope, and funding for radioastronomy facilities and projects. (JN)

Sanford Underground Research Facility - The United State's Deep Underground Research Facility

Science.gov (United States)

Vardiman, D.

2012-12-01

The 2.5 km deep Sanford Underground Research Facility (SURF) is managed by the South Dakota Science and Technology Authority (SDSTA) at the former Homestake Mine site in Lead, South Dakota. The US Department of Energy currently supports the development of the facility using a phased approach for underground deployment of experiments as they obtain an advanced design stage. The geology of the Sanford Laboratory site has been studied during the 125 years of operations at the Homestake Mine and more recently as part of the preliminary geotechnical site investigations for the NSF's Deep Underground Science and Engineering Laboratory project. The overall geology at DUSEL is a well-defined stratigraphic sequence of schist and phyllites. The three major Proterozoic units encountered in the underground consist of interbedded schist, metasediments, and amphibolite schist which are crosscut by Tertiary rhyolite dikes. Preliminary geotechnical site investigations included drift mapping, borehole drilling, borehole televiewing, in-situ stress analysis, laboratory analysis of core, mapping and laser scanning of new excavations, modeling and analysis of all geotechnical information. The investigation was focused upon the determination if the proposed site rock mass could support the world's largest (66 meter diameter) deep underground excavation. While the DUSEL project has subsequently been significantly modified, these data are still available to provide a baseline of the ground conditions which may be judiciously extrapolated throughout the entire Proterozoic rock assemblage for future excavations. Recommendations for facility instrumentation and monitoring were included in the preliminary design of the DUSEL project design and include; single and multiple point extensometers, tape extensometers and convergence measurements (pins), load cells and pressure cells, smart cables, inclinometers/Tiltmeters, Piezometers, thermistors, seismographs and accelerometers, scanners (laser

EPA Facility Registry System (FRS): NCES

Science.gov (United States)

This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link to the National Center for Education Statistics (NCES). The primary federal database for collecting and analyzing data related to education in the United States and other Nations, NCES is located in the U.S. Department of Education, within the Institute of Education Sciences. FRS identifies and geospatially locates facilities, sites or places subject to environmental regulations or of environmental interest. Using vigorous verification and data management procedures, FRS integrates facility data from EPA00e2??s national program systems, other federal agencies, and State and tribal master facility records and provides EPA with a centrally managed, single source of comprehensive and authoritative information on facilities. This data set contains the subset of FRS integrated facilities that link to NCES school facilities once the NCES data has been integrated into the FRS database. Additional information on FRS is available at the EPA website http://www.epa.gov/enviro/html/fii/index.html.

Trauma facilities in Denmark - A nationwide cross-sectional benchmark study of facilities and trauma care organisation

DEFF Research Database (Denmark)

Weile, Jesper; Nielsen, Klaus; Primdahl, Stine C.

2018-01-01

Background: Trauma is a leading cause of death among adults aged trauma facilities and the use multidisciplinary trauma teams. Because knowledge is sparse on the existing distribution of trauma facilities...... and the organisation of trauma care in Denmark, the aim of this study was to identify all Danish facilities that care for traumatized patients and to investigate the diversity in organization of trauma management. Methods: We conducted a systematic observational cross-sectional study. First, all hospitals in Denmark...... were identified via online services and clarifying phone calls to each facility. Second, all trauma care manuals on all facilities that receive traumatized patients were gathered. Third, anesthesiologists and orthopedic surgeons on call at all trauma facilities were contacted via telephone...

Survey of solar thermal test facilities

Energy Technology Data Exchange (ETDEWEB)

Masterson, K.

1979-08-01

The facilities that are presently available for testing solar thermal energy collection and conversion systems are briefly described. Facilities that are known to meet ASHRAE standard 93-77 for testing flat-plate collectors are listed. The DOE programs and test needs for distributed concentrating collectors are identified. Existing and planned facilities that meet these needs are described and continued support for most of them is recommended. The needs and facilities that are suitable for testing components of central receiver systems, several of which are located overseas, are identified. The central contact point for obtaining additional details and test procedures for these facilities is the Solar Thermal Test Facilities Users' Association in Albuquerque, N.M. The appendices contain data sheets and tables which give additional details on the technical capabilities of each facility. Also included is the 1975 Aerospace Corporation report on test facilities that is frequently referenced in the present work.

20 CFR 404.1623 - Facilities.

Science.gov (United States)

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Facilities. 404.1623 Section 404.1623...- ) Determinations of Disability Administrative Responsibilities and Requirements § 404.1623 Facilities. (a) Space... determinations. (b) Location of facilities. Subject to appropriate Federal funding, the State will determine the...

Capital Ideas for Facilities Management.

Science.gov (United States)

Golding, Stephen T.; Gordon, Janet; Gravina, Arthur

2001-01-01

Asserting that just like chief financial officers, higher education facilities specialists must maximize the long-term performance of assets under their care, describes strategies for strategic facilities management. Discusses three main approaches to facilities management (insourcing, cosourcing, and outsourcing) and where boards of trustees fit…

Medical cyclotron facilities

International Nuclear Information System (INIS)

1984-09-01

This report examines the separate proposals from the Austin Hospital and the Australian Atomic Energy Commission for a medical cyclotron facility. The proponents have argued that a cyclotron facility would benefit Australia in areas of patient care, availability and export of radioisotopes, and medical research. Positron emission tomography (PET) and neutron beam therapy are also examined

Facility planning and site development

International Nuclear Information System (INIS)

Reisman, R.C.; Handmaker, H.

1986-01-01

Planning for a magnetic resonance imaging (MRI) facility should provide for the efficient operation of current and future MRI devices and must also take into consideration a broad range of general planning principles. Control of budgeted facility costs and construction schedules is of increasing importance due to the magnitude of expense of MRI facility development as well as the need to protect institutional or entrepreneurial investment. In a competitive environment facility costs may be the determining factor in a project's success

European Synchrotron Radiation Facility

International Nuclear Information System (INIS)

Buras, B.

1985-01-01

How a European Synchrotron Radiation Facility has developed into a detailed proposal recently accepted as the basis for construction of the facility at Grenoble is discussed. In November 1977, the General Assembly of the European Science Foundation (ESF) approved the report of the ESF working party on synchrotron radiation entitled Synchrotron Radiation - a Perspective View for Europe. This report contained as one of its principal recommendations that work should commence on a feasibility study for a European synchrotron radiation laboratory having a dedicated hard X-ray storage ring and appropriate advanced instrumentation. In order to prepare a feasibility study the European Science Foundation set up the Ad-hoc Committee on Synchrotron Radiation, which in turn formed two working groups: one for the machine and another for instrumentation. This feasibility study was completed in 1979 with the publication of the Blue Book describing in detail the so called 1979 European Synchrotron Radiation Facility. The heart of the facility was a 5 GeV electron storage ring and it was assumed that mainly the radiation from bending magnets will be used. The facility is described

Trauma facilities in Denmark - a nationwide cross-sectional benchmark study of facilities and trauma care organisation.

Science.gov (United States)

Weile, Jesper; Nielsen, Klaus; Primdahl, Stine C; Frederiksen, Christian A; Laursen, Christian B; Sloth, Erik; Mølgaard, Ole; Knudsen, Lars; Kirkegaard, Hans

2018-03-27

Trauma is a leading cause of death among adults aged facilities and the use multidisciplinary trauma teams. Because knowledge is sparse on the existing distribution of trauma facilities and the organisation of trauma care in Denmark, the aim of this study was to identify all Danish facilities that care for traumatized patients and to investigate the diversity in organization of trauma management. We conducted a systematic observational cross-sectional study. First, all hospitals in Denmark were identified via online services and clarifying phone calls to each facility. Second, all trauma care manuals on all facilities that receive traumatized patients were gathered. Third, anesthesiologists and orthopedic surgeons on call at all trauma facilities were contacted via telephone for structured interviews. A total of 22 facilities in Denmark were found to receive traumatized patients. All facilities used a trauma care manual and all had a multidisciplinary trauma team. The study found three different trauma team activation criteria and nine different compositions of teams who participate in trauma care. Training was heterogeneous and, beyond the major trauma centers, databases were only maintained in a few facilities. The study established an inventory of the existing Danish facilities that receive traumatized patients. The trauma team activation criteria and the trauma teams were heterogeneous in both size and composition. A national database for traumatized patients, research on nationwide trauma team activation criteria, and team composition guidelines are all called for.

7 CFR 51.57 - Facilities.

Science.gov (United States)

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Facilities. 51.57 Section 51.57 Agriculture... Requirements for Plants Operating Under Continuous Inspection on A Contract Basis § 51.57 Facilities. Each packing plant shall be equipped with adequate sanitary facilities and accommodations, including but not...

50 CFR 260.100 - Facilities.

Science.gov (United States)

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Facilities. 260.100 Section 260.100... Basis 1 § 260.100 Facilities. Each official establishment shall be equipped with adequate sanitary facilities and accommodations, including, but not being limited to, the following: (a) Containers approved...

Liquefied Gaseous Fuels Spill Test Facility

International Nuclear Information System (INIS)

1993-02-01

The US Department of Energy's liquefied Gaseous Fuels Spill Test Facility is a research and demonstration facility available on a user-fee basis to private and public sector test and training sponsors concerned with safety aspects of hazardous chemicals. Though initially designed to accommodate large liquefied natural gas releases, the Spill Test Facility (STF) can also accommodate hazardous materials training and safety-related testing of most chemicals in commercial use. The STF is located at DOE's Nevada Test Site near Mercury, Nevada, USA. Utilization of the Spill Test Facility provides a unique opportunity for industry and other users to conduct hazardous materials testing and training. The Spill Test Facility is the only facility of its kind for either large- or small-scale testing of hazardous and toxic fluids including wind tunnel testing under controlled conditions. It is ideally suited for test sponsors to develop verified data on prevention, mitigation, clean-up, and environmental effects of toxic and hazardous gaseous liquids. The facility site also supports structured training for hazardous spills, mitigation, and clean-up. Since 1986, the Spill Test Facility has been utilized for releases to evaluate the patterns of dispersion, mitigation techniques, and combustion characteristics of select materials. Use of the facility can also aid users in developing emergency planning under US P.L 99-499, the Superfund Amendments and Reauthorization Act of 1986 (SARA) and other regulations. The Spill Test Facility Program is managed by the US Department of Energy (DOE), Office of Fossil Energy (FE) with the support and assistance of other divisions of US DOE and the US Government. DOE/FE serves as facilitator and business manager for the Spill Test Facility and site. This brief document is designed to acquaint a potential user of the Spill Test Facility with an outline of the procedures and policies associated with the use of the facility

44 CFR 331.5 - Production facilities.

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Production facilities. 331.5... AND FACILITIES IN LABOR SURPLUS AREAS § 331.5 Production facilities. All Federal departments and... production facilities, including expansion, to the extent that such selection is consistent with existing law...

28 CFR 54.410 - Comparable facilities.

Science.gov (United States)

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Comparable facilities. 54.410 Section 54... in Education Programs or Activities Prohibited § 54.410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities...

Facilities Performance Indicators Report, 2008-09

Science.gov (United States)

Hills, Christina, Ed.

2010-01-01

This paper features another expanded Web-based Facilities Performance Indicators Report (FPI). The purpose of APPA's Facilities Performance Indicators is to provide a representative set of statistics about facilities in educational institutions. The 2008-09 iteration of the Web-based Facilities Performance Indicators Survey was posted and…

Facility Management's Role in Organizational Sustainability

Science.gov (United States)

Adams, Gregory K.

2013-01-01

Facility managers have questions about sustainability. How do an organization's physical facilities--its built environment--and the management of them, influence the sustainability of the organization or institution as a whole? How important is Facility Management (FM) to the overall sustainability profile of an organization? Facility managers…

Fusion Materials Irradiation Test Facility: a facility for fusion-materials qualification

International Nuclear Information System (INIS)

Trego, A.L.; Hagan, J.W.; Opperman, E.K.; Burke, R.J.

1983-01-01

The Fusion Materials Irradiation Test Facility will provide a unique testing environment for irradiation of structural and special purpose materials in support of fusion power systems. The neutron source will be produced by a deuteron-lithium stripping reaction to generate high energy neutrons to ensure damage similar to that of a deuterium-tritium neutron spectrum. The facility design is now ready for the start of construction and much of the supporting lithium system research has been completed. Major testing of key low energy end components of the accelerator is about to commence. The facility, its testing role, and the status and major aspects of its design and supporting system development are described

Designing Facilities for Collaborative Operations

Science.gov (United States)

Norris, Jeffrey; Powell, Mark; Backes, Paul; Steinke, Robert; Tso, Kam; Wales, Roxana

2003-01-01

A methodology for designing operational facilities for collaboration by multiple experts has begun to take shape as an outgrowth of a project to design such facilities for scientific operations of the planned 2003 Mars Exploration Rover (MER) mission. The methodology could also be applicable to the design of military "situation rooms" and other facilities for terrestrial missions. It was recognized in this project that modern mission operations depend heavily upon the collaborative use of computers. It was further recognized that tests have shown that layout of a facility exerts a dramatic effect on the efficiency and endurance of the operations staff. The facility designs (for example, see figure) and the methodology developed during the project reflect this recognition. One element of the methodology is a metric, called effective capacity, that was created for use in evaluating proposed MER operational facilities and may also be useful for evaluating other collaboration spaces, including meeting rooms and military situation rooms. The effective capacity of a facility is defined as the number of people in the facility who can be meaningfully engaged in its operations. A person is considered to be meaningfully engaged if the person can (1) see, hear, and communicate with everyone else present; (2) see the material under discussion (typically data on a piece of paper, computer monitor, or projection screen); and (3) provide input to the product under development by the group. The effective capacity of a facility is less than the number of people that can physically fit in the facility. For example, a typical office that contains a desktop computer has an effective capacity of .4, while a small conference room that contains a projection screen has an effective capacity of around 10. Little or no benefit would be derived from allowing the number of persons in an operational facility to exceed its effective capacity: At best, the operations staff would be underutilized

14 CFR 142.15 - Facilities.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Facilities. 142.15 Section 142.15... OTHER CERTIFICATED AGENCIES TRAINING CENTERS General § 142.15 Facilities. (a) An applicant for, or..., sanitation, and health codes; and (2) The facilities used for instruction are not routinely subject to...

Concrete structures for nuclear facilities

International Nuclear Information System (INIS)

1996-01-01

The detailed requirements for the design and fabrication of the concrete structures for nuclear facilities and for the documents to be submitted to the Finnish Centre for Radiation and Nuclear Safety (STUK) are given in the guide. It also sets the requirements for the inspection of concrete structures during the construction and operation of facilities. The requirements of the guide primarily apply to new construction. As regards the repair and modification of nuclear facilities built before its publication, the guide is followed to the extent appropriate. The regulatory activities of the Finnish Centre for Radiation and Nuclear Safety during a nuclear facility's licence application review and during the construction and operation of the facility are summarised in the guide YVL 1.1

30 CFR 57.20008 - Toilet facilities.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Toilet facilities. 57.20008 Section 57.20008....20008 Toilet facilities. (a) Toilet facilities shall be provided at locations that are compatible with the mine operations and that are readily accessible to mine personnel. (b) The facilities shall be...

30 CFR 57.6161 - Auxiliary facilities.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Auxiliary facilities. 57.6161 Section 57.6161...-Underground Only § 57.6161 Auxiliary facilities. (a) Auxiliary facilities used to store explosive material near work places shall be wooden, box-type containers equipped with covers or doors, or facilities...

14 CFR 1251.301 - Existing facilities.

Science.gov (United States)

2010-01-01

... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Existing facilities. 1251.301 Section 1251... HANDICAP Accessibility § 1251.301 Existing facilities. (a) Accessibility. A recipient shall operate each... existing facilities or every part of a facility accessible to and usable by handicapped persons. (b...

9 CFR 3.102 - Facilities, indoor.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Facilities, indoor. 3.102 Section 3... Marine Mammals Facilities and Operating Standards § 3.102 Facilities, indoor. (a) Ambient temperature. The air and water temperatures in indoor facilities shall be sufficiently regulated by heating or...

36 CFR 13.166 - Temporary facilities.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false Temporary facilities. 13.166... facilities. A temporary facility or structure directly and necessarily related to the taking of subsistence... facilities which shall be published annually in accordance with § 1.7 of this chapter. ...

Cold Vacuum Drying Facility

Data.gov (United States)

Federal Laboratory Consortium — Located near the K-Basins (see K-Basins link) in Hanford's 100 Area is a facility called the Cold Vacuum Drying Facility (CVDF).Between 2000 and 2004, workers at the...

JPRS Report Near East & South Asia

Science.gov (United States)

1991-02-22

streets of Yerevan has not forgotten the past and the genocide and keeps his eyes fixed on Mount Ararat . Birand is not mistaken. It is Yesayi...five frozen accounts—Lotus, Tulip, Mont Blanc, Svenska Inc and an unidentified account—be passed on to the Indian investigators. Even if the Cantonal...accounts of ’Tulip,’ ’Lotus’ and ’ Mont Blanc’ (these are accused numbers 11, 12 and 13 respectively), and certain public servants of the government

The Birmingham Irradiation Facility

International Nuclear Information System (INIS)

Dervan, P.; French, R.; Hodgson, P.; Marin-Reyes, H.; Wilson, J.

2013-01-01

At the end of 2012 the proton irradiation facility at the CERN PS will shut down for two years. With this in mind, we have been developing a new ATLAS scanning facility at the University of Birmingham Medical Physics cyclotron. With proton beams of energy approximately 30 MeV, fluences corresponding to those of the upgraded Large Hadron Collider (HL-LHC) can be reached conveniently. The facility can be used to irradiate silicon sensors, optical components and mechanical structures (e.g. carbon fibre sandwiches) for the LHC upgrade programme. Irradiations of silicon sensors can be carried out in a temperature controlled cold box that can be scanned through the beam. The facility is described in detail along with the first tests carried out with mini (1×1 cm 2 ) silicon sensors

The Birmingham Irradiation Facility

CERN Document Server

Dervan, P; Hodgson, P; Marin-Reyes, H; Wilson, J

2013-01-01

At the end of 2012 the proton irradiation facility at the CERN PS [1] will shut down for two years. With this in mind, we have been developing a new ATLAS scanning facility at the University of Birmingham Medical Physics cyclotron. With proton beams of energy approximately 30 MeV, fluences corresponding to those of the upgraded Large Hadron Collider (HL-LHC) can be reached conveniently. The facility can be used to irradiate silicon sensors, optical components and mechanical structures (e.g. carbon fibre sandwiches) for the LHC upgrade programme. Irradiations of silicon sensors can be carried out in a temperature controlled cold box that can be scanned through the beam. The facility is described in detail along with the first tests carried out with mini (1 x 1 cm^2 ) silicon sensors.

DUPIC facility engineering

International Nuclear Information System (INIS)

Park, J. J.; Lee, H. H.; Kim, K. H.

2002-03-01

With starting DUPIC fuel fabrication experiment by using spent fuels, 1) operation and refurbishment for DFDF (DUPIC fuel development facility), and 2) operation and improvement of transportation equipment for radioactive materials between facilities became the objectives of this study. This report describes objectives of the project, necessities, state of related technology, R and D scope, R and D results, proposal for application etc

PRTR/309 building nuclear facility preliminary

International Nuclear Information System (INIS)

Cornwell, B.C.

1994-01-01

The hazard classification of the Plutonium Recycle Test Reactor (PRTR)/309 building as a ''Radiological Facility'' and the office portions as ''Other Industrial Facility'' are documented by this report. This report provides: a synopsis of the history and facility it's uses; describes major area of the facility; and assesses the radiological conditions for the facility segments. The assessment is conducted using the hazard category threshold values, segmentation methodology, and graded approach guidance of DOE-STD-1027-92

The ORION Facility

International Nuclear Information System (INIS)

Noble, Robert

2003-01-01

ORION will be a user-oriented research facility for understanding the physics and developing the technology for future high-energy particle accelerators, as well as for research in related fields. The facility has as its centerpiece the Next Linear Collider Test Accelerator (NLCTA) at the Stanford Linear Accelerator Center (SLAC). The NLCTA will be modified with the addition of a new, high-brightness photoinjector, its drive laser, an S-band rf power system, a user laser room, a low-energy experimental hall supplied with electron beams up to 60 MeV in energy, and a high-energy hall supplied with beams up to 350 MeV. The facility design and parameters are described here along with highlights from the 2nd ORION Workshop held in February 2003

Species-specific effects of live roots and shoot litter on soil decomposer abundances do not forecast plant litter-nitrogen uptake

DEFF Research Database (Denmark)

Saj, Stéphane; Mikola, Juha; Ekelund, Flemming

2009-01-01

and bacterial- and fungal-feeding nematodes did not differ between the live plants, litter-N uptake differed, with Holcus being the most efficient compared to Plantago and Lotus. Hence, although microbes and their feeders unquestionably control N mineralization in the soil, and their growth differs among plant......Plant species produce litter of varying quality and differ in the quality and quantity of compounds they release from live roots, which both can induce different decomposer growth in the soil. To test whether differences in decomposer growth can forecast the amount of N species acquire from plant...... litter, as suggested by theory, we grew individuals of three grassland plants-Holcus lanatus, Plantago lanceolata and Lotus corniculatus-in soils into which (15)N-labelled litter of either Holcus, Plantago or Lotus was added. We measured the effects of live roots and litter of each species on soil...

Ouellette Thermal Test Facility

Data.gov (United States)

Federal Laboratory Consortium — The Thermal Test Facility is a joint Army/Navy state-of-the-art facility (8,100 ft2) that was designed to:Evaluate and characterize the effect of flame and thermal...

30 CFR 816.181 - Support facilities.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Support facilities. 816.181 Section 816.181... § 816.181 Support facilities. (a) Support facilities shall be operated in accordance with a permit... results. (b) In addition to the other provisions of this part, support facilities shall be located...

10 CFR 4.127 - Existing facilities.

Science.gov (United States)

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Existing facilities. 4.127 Section 4.127 Energy NUCLEAR... 1973, as Amended Discriminatory Practices § 4.127 Existing facilities. (a) Accessibility. A recipient... make each of its existing facilities or every part of an existing facility accessible to and usable by...

30 CFR 57.6160 - Main facilities.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Main facilities. 57.6160 Section 57.6160...-Underground Only § 57.6160 Main facilities. (a) Main facilities used to store explosive material underground... facilities will not prevent escape from the mine, or cause detonation of the contents of another storage...

45 CFR 1170.32 - Existing facilities.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Existing facilities. 1170.32 Section 1170.32... ASSISTED PROGRAMS OR ACTIVITIES Accessibility § 1170.32 Existing facilities. (a) Accessibility. A recipient... require a recipient to make each of its existing facilities or every part of a facility accessible to and...

30 CFR 56.20008 - Toilet facilities.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Toilet facilities. 56.20008 Section 56.20008... Toilet facilities. (a) Toilet facilities shall be provided at locations that are compatible with the mine operations and that are readily accessible to mine personnel. (b) The facilities shall be kept clean and...

45 CFR 605.22 - Existing facilities.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Existing facilities. 605.22 Section 605.22 Public... Accessibility § 605.22 Existing facilities. (a) Accessibility. A recipient shall operate each program or... existing facilities or every part of a facility accessible to and usable by qualified handicapped persons...

32 CFR 196.410 - Comparable facilities.

Science.gov (United States)

2010-07-01

... 32 National Defense 2 2010-07-01 2010-07-01 false Comparable facilities. 196.410 Section 196.410....410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities provided for students of one sex shall be comparable to such...

9 CFR 3.25 - Facilities, general.

Science.gov (United States)

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Facilities, general. 3.25 Section 3.25... Pigs and Hamsters Facilities and Operating Standards § 3.25 Facilities, general. (a) Structural strength. Indoor and outdoor housing facilities for guinea pigs or hamsters shall be structurally sound and...

10 CFR 1040.72 - Existing facilities.

Science.gov (United States)

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Existing facilities. 1040.72 Section 1040.72 Energy... § 1040.72 Existing facilities. (a) Accessibility. A recipient shall operate any program or activity to... facilities or every part of a facility accessible to and useable by handicapped persons. (b) Methods. A...

45 CFR 1151.22 - Existing facilities.

Science.gov (United States)

2010-10-01

... 45 Public Welfare 3 2010-10-01 2010-10-01 false Existing facilities. 1151.22 Section 1151.22... Prohibited Accessibility § 1151.22 Existing facilities. (a) A recipient shall operate each program or... make each of its existing facilities or every part of a facility accessible to and usable by...

30 CFR 817.181 - Support facilities.

Science.gov (United States)

2010-07-01

... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Support facilities. 817.181 Section 817.181... ACTIVITIES § 817.181 Support facilities. (a) Support facilities shall be operated in accordance with a permit.... (b) In addition to the other provisions of this part, support facilities shall be located, maintained...

36 CFR 1211.410 - Comparable facilities.

Science.gov (United States)

2010-07-01

... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Comparable facilities. 1211... § 1211.410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities provided for students of one sex shall be comparable to...

10 CFR 1042.410 - Comparable facilities.

Science.gov (United States)

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Comparable facilities. 1042.410 Section 1042.410 Energy... Activities Prohibited § 1042.410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities provided for students of one sex shall...

44 CFR 19.410 - Comparable facilities.

Science.gov (United States)

2010-10-01

... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Comparable facilities. 19.410... Activities Prohibited § 19.410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities provided for students of one sex shall...

Establishment and Operation of User Facilities

International Nuclear Information System (INIS)

Cho, Yong Sub; Kwon, Hyeok Jung; Kim, Kye Ryung

2008-05-01

PEFP(Proton Engineering Frontier Project) has launched on a new enterprise to develop the technologies for the future relating to the proton beam and spin-off technologies in 2002. PEFP planned to supply 20MeV and 100MeV proton beam by the development of the 100MeV, 20mA linear accelerator during ten years from 2002 to 2012. The final goal of this project is establishment of 20MeV and 100MeV user facilities. To do this, we must develop the key technologies for establishing user facilities. Before the main facilities are normally operated, we have established the test user facilities to support various kinds of users' basic experiments and pilot studies. The necessity of this research are as follows; - Domestic achievement of key technologies for the development and design of the user facilities for the several tens to hundreds MeV class high current proton beam - Beam application researches can be revitalized and improved the efficiency by the establishment and operation of user facilities and test facilities. - Ion implantation facilities have contributed to increase Industrial applications - It is more effective in saving money that users use the PEFP's user facility than other country's user facilities. - It is possible to contribute to the local society and commercialize the beam application technologies by the establishment of PEFP's research branch in Kyungju

Making of the NSTX Facility

International Nuclear Information System (INIS)

Neumeyer, C.; Ono, M.; Kaye, S.M.; Peng, Y.-K.M.

1999-01-01

The NSTX (National Spherical Torus Experiment) facility located at Princeton Plasma Physics Laboratory is the newest national fusion science experimental facility for the restructured US Fusion Energy Science Program. The NSTX project was approved in FY 97 as the first proof-of-principle national fusion facility dedicated to the spherical torus research. On Feb. 15, 1999, the first plasma was achieved 10 weeks ahead of schedule. The project was completed on budget and with an outstanding safety record. This paper gives an overview of the NSTX facility construction and the initial plasma operations

Needs of Advanced Safeguards Technologies for Future Nuclear Fuel Cycle (FNFC) Facilities and a Trial Application of SBD Concept to Facility Design of a Hypothetical FNFC Facility

International Nuclear Information System (INIS)

Seya, M.; Hajima, R.; Nishimori, N.; Hayakawa, T.; Kikuzawa, N.; Shizuma, T.; Fujiwara, M.

2010-01-01

Some of future nuclear fuel cycle (FNFC) facilities are supposed to have the characteristic features of very large throughput of plutonium, low decontamination reprocessing (no purification process; existence of certain amount of fission products (FP) in all process material), full minor actinides (MA) recycle, and treatment of MOX with FP and MA in fuel fabrication. In addition, the following international safeguards requirements have to be taken into account for safeguards approaches of the FNFC facilities. -Application of integrated safeguards (IS) approach; -Remote (unattended) verification; - 'Safeguards by Design' (SBD) concept. These features and requirements compel us to develop advanced technologies, which are not emerged yet. In order to realize the SBD, facility designers have to know important parts of design information on advanced safeguards systems before starting the facility design. The SBD concept requires not only early start of R and D of advanced safeguards technologies (before starting preliminary design of the facility) but also interaction steps between researchers working on safeguards systems and nuclear facility designers. The interaction steps are follows. Step-1; researchers show images of advanced safeguards systems to facility designers based on their research. Step-2; facility designers take important design information on safeguards systems into process systems of demonstration (or test) facility. Step-3; demonstration and improvement of both systems based on the conceptual design. Step-4; Construction of a FNFC facility with the advanced safeguards systems We present a trial application of the SBD concept to a hypothetical FNFC facility with an advanced hybrid K-edge densitometer and a Pu NDA system for spent nuclear fuel assembly using laser Compton scattering (LCS) X-rays and γ-rays and other advanced safeguards systems. (author)

Neighbourhood facilities for sustainability

CSIR Research Space (South Africa)

Gibberd, Jeremy T

2013-01-01

Full Text Available . In this paper these are referred to as ‘Neighbourhood Facilities for Sustainability’. Neighbourhood Facilities for Sustainability (NFS) are initiatives undertaken by individuals and communities to build local sustainable systems which not only improve...

340 Waste Handling Facility interim safety basis

International Nuclear Information System (INIS)

Bendixsen, R.B.

1995-01-01

This document establishes the interim safety basis (ISB) for the 340 Waste Handling Facility (340 Facility). An ISB is a documented safety basis that provides a justification for the continued operation of the facility until an upgraded final safety analysis report is prepared that complies with US Department of Energy (DOE) Order 5480.23, Nuclear Safety Analysis Reports. The ISB for the 340 Facility documents the current design and operation of the facility. The 340 Facility ISB (ISB-003) is based on a facility walkdown and review of the design and operation of the facility, as described in the existing safety documentation. The safety documents reviewed, to develop ISB-003, include the following: OSD-SW-153-0001, Operating Specification Document for the 340 Waste Handling Facility (WHC 1990); OSR-SW-152-00003, Operating Limits for the 340 Waste Handling Facility (WHC 1989); SD-RE-SAP-013, Safety Analysis Report for Packaging, Railroad Liquid Waste Tank Cars (Mercado 1993); SD-WM-TM-001, Safety Assessment Document for the 340 Waste Handling Facility (Berneski 1994a); SD-WM-SEL-016, 340 Facility Safety Equipment List (Berneski 1992); and 340 Complex Fire Hazard Analysis, Draft (Hughes Assoc. Inc. 1994)

Facility Effluent Monitoring Plan for the 2724-W Protective Equipment Decontamination Facility

International Nuclear Information System (INIS)

Carter, G.J.

1991-11-01

A facility effluent monitoring plan is required by the US Department of Energy in DOE Order 5400.1* for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal, state, and local requirements. This facility effluent monitoring plan is the first annual report. It shall ensure long-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updates as a minimum every three years

10 CFR 5.410 - Comparable facilities.

Science.gov (United States)

2010-01-01

... 10 Energy 1 2010-01-01 2010-01-01 false Comparable facilities. 5.410 Section 5.410 Energy NUCLEAR... Prohibited § 5.410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities provided for students of one sex shall be comparable to...

Safety of magnetic fusion facilities: Guidance

International Nuclear Information System (INIS)

1996-05-01

This document provides guidance for the implementation of the requirements identified in DOE-STD-6002-96, Safety of Magnetic Fusion Facilities: Requirements. This guidance is intended for the managers, designers, operators, and other personnel with safety responsibilities for facilities designated as magnetic fusion facilities. While the requirements in DOE-STD-6002-96 are generally applicable to a wide range of fusion facilities, this Standard, DOE-STD-6003-96, is concerned mainly with the implementation of those requirements in large facilities such as the International Thermonuclear Experimental Reactor (ITER). Using a risk-based prioritization, the concepts presented here may also be applied to other magnetic fusion facilities. This Standard is oriented toward regulation in the Department of Energy (DOE) environment as opposed to regulation by other regulatory agencies. As the need for guidance involving other types of fusion facilities or other regulatory environments emerges, additional guidance volumes should be prepared. The concepts, processes, and recommendations set forth here are for guidance only. They will contribute to safety at magnetic fusion facilities

21 CFR 1271.190 - Facilities.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Facilities. 1271.190 Section 1271.190 Food and... CELLULAR AND TISSUE-BASED PRODUCTS Current Good Tissue Practice § 1271.190 Facilities. (a) General. Any facility used in the manufacture of HCT/Ps must be of suitable size, construction, and location to prevent...

Samarbejdsformer og Facilities Management

DEFF Research Database (Denmark)

Storgaard, Kresten

Resultater fra en surveyundersøgelse om fordele og ulemper ved forskellige samarbejdsformer indenfor Facilities Management fremlægges.......Resultater fra en surveyundersøgelse om fordele og ulemper ved forskellige samarbejdsformer indenfor Facilities Management fremlægges....

Establishment and Operation of User Facilities

International Nuclear Information System (INIS)

Kim, Kye Ryung; Park, B. S.; Lim, Y. K.; Lee, S. K.; Jung, J. P.

2005-08-01

The final goal of this project is to establish the proton beam user facility which can offer the suitable proton beam for the user's demand. In the first phase we developed the key technologies that were required for the establishment of 20MeV and 100MeV proton user facilities. The user's demand survey was also achieved, and the test user facility was established on the results of the demand survey. Using the test facility, the users performed their pilot studies. Now, we have finished the conceptual design for 20MeV proton user facility. During the first phase we performed the user's demand survey and produced many materials related to the proton beam utilizations in domestic or abroad. The survey results were reflected on the establishment of the test user facility and the conceptual design of 20MeV/100MeV proton beam user facilities. We have developed the key technologies which concern to beam energy control, flux control, uniform irradiation, dose and uniformity measurement, proton energy measurement, SOBP(Spread-out Bragg Peak) system using a rotating range modulator, and carried out the conceptual design of 20MeV proton user facility. The test user facility has been constructed and operated for both verifying the developed key technologies and performing the user's preliminary experiments. 45MeV low flux user facility was constructed in 2003 and has performed a lot of irradiation experiments. The development of 1.8MeV test user facility was completed. Also the low energy user facility that KAERI kept was upgraded and used for many users. Therefore, we provided our users with various beams. On the other hand, the following activities were carried out, such as, inviting the oversea researchers, giving support to users to use the beam in domestic and abroad, discussing the beam utilization technologies by visiting the foreign user facilities, etc

Berkeley Low Background Facility

International Nuclear Information System (INIS)

Thomas, K. J.; Norman, E. B.; Smith, A. R.; Poon, A. W. P.; Chan, Y. D.; Lesko, K. T.

2015-01-01

The Berkeley Low Background Facility (BLBF) at Lawrence Berkeley National Laboratory (LBNL) in Berkeley, California provides low background gamma spectroscopy services to a wide array of experiments and projects. The analysis of samples takes place within two unique facilities; locally within a carefully-constructed, low background laboratory on the surface at LBNL and at the Sanford Underground Research Facility (SURF) in Lead, SD. These facilities provide a variety of gamma spectroscopy services to low background experiments primarily in the form of passive material screening for primordial radioisotopes (U, Th, K) or common cosmogenic/anthropogenic products; active screening via neutron activation analysis for U,Th, and K as well as a variety of stable isotopes; and neutron flux/beam characterization measurements through the use of monitors. A general overview of the facilities, services, and sensitivities will be presented. Recent activities and upgrades will also be described including an overview of the recently installed counting system at SURF (recently relocated from Oroville, CA in 2014), the installation of a second underground counting station at SURF in 2015, and future plans. The BLBF is open to any users for counting services or collaboration on a wide variety of experiments and projects

Subsurface Facility System Description Document

International Nuclear Information System (INIS)

Eric Loros

2001-01-01

The Subsurface Facility System encompasses the location, arrangement, size, and spacing of the underground openings. This subsurface system includes accesses, alcoves, and drifts. This system provides access to the underground, provides for the emplacement of waste packages, provides openings to allow safe and secure work conditions, and interfaces with the natural barrier. This system includes what is now the Exploratory Studies Facility. The Subsurface Facility System physical location and general arrangement help support the long-term waste isolation objectives of the repository. The Subsurface Facility System locates the repository openings away from main traces of major faults, away from exposure to erosion, above the probable maximum flood elevation, and above the water table. The general arrangement, size, and spacing of the emplacement drifts support disposal of the entire inventory of waste packages based on the emplacement strategy. The Subsurface Facility System provides access ramps to safely facilitate development and emplacement operations. The Subsurface Facility System supports the development and emplacement operations by providing subsurface space for such systems as ventilation, utilities, safety, monitoring, and transportation

Hanford surplus facilities hazards identification document

International Nuclear Information System (INIS)

Egge, R.G.

1997-01-01

This document provides general safety information needed by personnel who enter and work in surplus facilities managed by Bechtel Hanford, Inc. The purpose of the document is to enhance access control of surplus facilities, educate personnel on the potential hazards associated with these facilities prior to entry, and ensure that safety precautions are taken while in the facility

30 CFR 71.400 - Bathing facilities; change rooms; sanitary flush toilet facilities.

Science.gov (United States)

2010-07-01

... flush toilet facilities. 71.400 Section 71.400 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY HEALTH STANDARDS-SURFACE COAL MINES AND SURFACE... installations and at the surface worksites of such mine. (Note: Sanitary facilities at surface work areas of...

Hot-cell verification facility

International Nuclear Information System (INIS)

Eschenbaum, R.A.

1981-01-01

The Hot Cell Verification Facility (HCVF) was established as the test facility for the Fuels and Materials Examination Facility (FMEF) examination equipment. HCVF provides a prototypic hot cell environment to check the equipment for functional and remote operation. It also provides actual hands-on training for future FMEF Operators. In its two years of operation, HCVF has already provided data to make significant changes in items prior to final fabrication. It will also shorten the startup time in FMEF since the examination equipment will have been debugged and operated in HCVF

Assisted Living Facilities - CARE_LONG_TERM_FACILITIES_ISDH_IN: Residential Care Facilities, Nursing Homes, and Hospices in Indiana in 2007 (Indiana State Department of Health, Point Shapefile)

Data.gov (United States)

NSGIC State | GIS Inventory — CARE_LONG_TERM_FACILITIES_ISDH_IN is a point shapefile showing the locations of 86 residential care facilities, 525 long-term care facilities (nursing homes), and 81...

Environmental restoration plan for the transfer of surplus facilities to the Facility Transition Program at Oak Ridge National Laboratory

International Nuclear Information System (INIS)

1995-08-01

This report will provide guidance on management, coordination, and integration of plans to transition facilities to the Facility Transition Program and activities as related to the Oak Ridge National Laboratory (ORNL) Environmental Restoration Program facilities. This report gives (1) guidance on the steps necessary for identifying ORNL surplus facilities, (2) interfaces of Surveillance and Maintenance (S and M) and Isotope Facility Deactivation program managers, (3) roles and responsibilities of the facility managers, and (4) initial S and M requirements upon acceptance into the Facility Transition Program

EPA Facility Registry Service (FRS): NCDB

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): BRAC

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): BIA

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RADINFO

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

Aircraft Test & Evaluation Facility (Hush House)

Data.gov (United States)

Federal Laboratory Consortium — The Aircraft Test and Evaluation Facility (ATEF), or Hush House, is a noise-abated ground test sub-facility. The facility's controlled environment provides 24-hour...

EPA Facility Registry Service (FRS): TRI

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): ICIS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RBLC

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry System (FRS): NCES

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): NEI

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): CAMDBS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry System (FRS): NEPT

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link...

48 CFR 970.3770 - Facilities management.

Science.gov (United States)

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Facilities management. 970... REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Facilities Management Contracting 970.3770 Facilities management. ...

EPA Facility Registry Service (FRS): SDWIS

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

EPA Facility Registry Service (FRS): RMP

Data.gov (United States)

U.S. Environmental Protection Agency — This web feature service contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link...

33-GVA interrupter test facility

International Nuclear Information System (INIS)

Parsons, W.M.; Honig, E.M.; Warren, R.W.

1979-01-01

The use of commercial ac circuit breakers for dc switching operations requires that they be evaluated to determine their dc limitations. Two 2.4-GVA facilities have been constructed and used for this purpose at LASL during the last several years. In response to the increased demand on switching technology, a 33-GVA facility has been constructed. Novel features incorporated into this facility include (1) separate capacitive and cryogenic inductive energy storage systems, (2) fiber-optic controls and optically-coupled data links, and (3) digital data acquisition systems. Facility details and planned tests on an experimental rod-array vacuum interrupter are presented

Facility Interface Capability Assessment (FICA) project report

International Nuclear Information System (INIS)

Pope, R.B.; MacDonald, R.R.; Viebrock, J.M.; Mote, N.

1995-09-01

The US Department of Energy's (DOE) Office of Civilian Radioactive Waste Management (OCRWM) is responsible for developing the Civilian Radioactive Waste Management System (CRWMS) to accept spent nuclear fuel from commercial facilities. The objective of the Facility Interface Capability Assessment (FICA) project was to assess the capability of each commercial spent nuclear fuel (SNF) storage facility, at which SNF is stored, to handle various SNF shipping casks. The purpose of this report is to present and analyze the results of the facility assessments completed within the FICA project. During Phase 1, the data items required to complete the facility assessments were identified and the database for the project was created. During Phase 2, visits were made to 122 facilities on 76 sites to collect data and information, the database was updated, and assessments of the cask-handling capabilities at each facility were performed. Each assessment of cask-handling capability contains three parts: the current capability of the facility (planning base); the potential enhanced capability if revisions were made to the facility licensing and/or administrative controls; and the potential enhanced capability if limited physical modifications were made to the facility. The main conclusion derived from the planning base assessments is that the current facility capabilities will not allow handling of any of the FICA Casks at 49 of the 122 facilities evaluated. However, consideration of potential revisions and/or modifications showed that all but one of the 49 facilities could be adapted to handle at least one of the FICA Casks. For this to be possible, facility licensing, administrative controls, and/or physical aspects of the facility would need to be modified

Facility Interface Capability Assessment (FICA) project report

Energy Technology Data Exchange (ETDEWEB)

Pope, R.B. [ed.] [Oak Ridge National Lab., TN (United States); MacDonald, R.R. [ed.] [Civilian Radioactive Waste Management System, Vienna, VA (United States); Viebrock, J.M.; Mote, N. [Nuclear Assurance Corp., Norcross, GA (United States)

1995-09-01

The US Department of Energy`s (DOE) Office of Civilian Radioactive Waste Management (OCRWM) is responsible for developing the Civilian Radioactive Waste Management System (CRWMS) to accept spent nuclear fuel from commercial facilities. The objective of the Facility Interface Capability Assessment (FICA) project was to assess the capability of each commercial spent nuclear fuel (SNF) storage facility, at which SNF is stored, to handle various SNF shipping casks. The purpose of this report is to present and analyze the results of the facility assessments completed within the FICA project. During Phase 1, the data items required to complete the facility assessments were identified and the database for the project was created. During Phase 2, visits were made to 122 facilities on 76 sites to collect data and information, the database was updated, and assessments of the cask-handling capabilities at each facility were performed. Each assessment of cask-handling capability contains three parts: the current capability of the facility (planning base); the potential enhanced capability if revisions were made to the facility licensing and/or administrative controls; and the potential enhanced capability if limited physical modifications were made to the facility. The main conclusion derived from the planning base assessments is that the current facility capabilities will not allow handling of any of the FICA Casks at 49 of the 122 facilities evaluated. However, consideration of potential revisions and/or modifications showed that all but one of the 49 facilities could be adapted to handle at least one of the FICA Casks. For this to be possible, facility licensing, administrative controls, and/or physical aspects of the facility would need to be modified.

Advanced reactor experimental facilities

International Nuclear Information System (INIS)

Amri, A.; Papin, J.; Uhle, J.; Vitanza, C.

2010-01-01

For many years, the NEA has been examining advanced reactor issues and disseminating information of use to regulators, designers and researchers on safety issues and research needed. Following the recommendation of participants at an NEA workshop, a Task Group on Advanced Reactor Experimental Facilities (TAREF) was initiated with the aim of providing an overview of facilities suitable for carrying out the safety research considered necessary for gas-cooled reactors (GCRs) and sodium fast reactors (SFRs), with other reactor systems possibly being considered in a subsequent phase. The TAREF was thus created in 2008 with the following participating countries: Canada, the Czech Republic, Finland, France, Germany, Hungary, Italy, Japan, Korea and the United States. In a second stage, India provided valuable information on its experimental facilities related to SFR safety research. The study method adopted entailed first identifying high-priority safety issues that require research and then categorizing the available facilities in terms of their ability to address the safety issues. For each of the technical areas, the task members agreed on a set of safety issues requiring research and established a ranking with regard to safety relevance (high, medium, low) and the status of knowledge based on the following scale relative to full knowledge: high (100%-75%), medium (75 - 25%) and low (25-0%). Only the issues identified as being of high safety relevance and for which the state of knowledge is low or medium were included in the discussion, as these issues would likely warrant further study. For each of the safety issues, the TAREF members identified appropriate facilities, providing relevant information such as operating conditions (in- or out-of reactor), operating range, description of the test section, type of testing, instrumentation, current status and availability, and uniqueness. Based on the information collected, the task members assessed prospects and priorities

Decommissioning engineering systems for nuclear facilities and knowledge inheritance for decommissioning of nuclear facilities

International Nuclear Information System (INIS)

Tachibana, Mitsuo

2016-01-01

Information on construction, operation and maintenance of a nuclear facility is essential in order to plan and implement the decommissioning of the nuclear facility. A decommissioning engineering system collects these information efficiently, retrieves necessary information rapidly, and support to plan the reasonable decommissioning as well as the systematic implementation of dismantling activities. Then, knowledge of workers involved facility operation and dismantling activities is important because decommissioning of nuclear facility will be carried out for a long period. Knowledge inheritance for decommissioning has been carried out in various organizations. This report describes an outline of and experiences in applying decommissioning engineering systems in JAEA and activities related to knowledge inheritance for decommissioning in some organizations. (author)

EPA Facility Registry Service (FRS): OIL

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset contains location and facility identification information from EPA's Facility Registry Service (FRS) for the subset of facilities that link to the Oil...

340 waste handling facility interim safety basis

Energy Technology Data Exchange (ETDEWEB)

VAIL, T.S.

1999-04-01

This document presents an interim safety basis for the 340 Waste Handling Facility classifying the 340 Facility as a Hazard Category 3 facility. The hazard analysis quantifies the operating safety envelop for this facility and demonstrates that the facility can be operated without a significant threat to onsite or offsite people.

340 waste handling facility interim safety basis

International Nuclear Information System (INIS)

VAIL, T.S.

1999-01-01

This document presents an interim safety basis for the 340 Waste Handling Facility classifying the 340 Facility as a Hazard Category 3 facility. The hazard analysis quantifies the operating safety envelop for this facility and demonstrates that the facility can be operated without a significant threat to onsite or offsite people

340 Facility maintenance implementation plan

International Nuclear Information System (INIS)

1995-03-01

This Maintenance Implementation Plan (MIP) has been developed for maintenance functions associated with the 340 Facility. This plan is developed from the guidelines presented by Department of Energy (DOE) Order 4330.4B, Maintenance Management Program (DOE 1994), Chapter II. The objective of this plan is to provide baseline information for establishing and identifying Westinghouse Hanford Company (WHC) conformance programs and policies applicable to implementation of DOE order 4330.4B guidelines. In addition, this maintenance plan identifies the actions necessary to develop a cost-effective and efficient maintenance program at the 340 Facility. Primary responsibility for the performance and oversight of maintenance activities at the 340 Facility resides with Westinghouse Hanford Company (WHC). Maintenance at the 340 Facility is performed by ICF-Kaiser Hanford (ICF-KH) South Programmatic Services crafts persons. This 340 Facility MIP provides interface requirements and responsibilities as they apply specifically to the 340 Facility. This document provides an implementation schedule which has been developed for items considered to be deficient or in need of improvement. The discussion sections, as applied to implementation at the 340 Facility, have been developed from a review of programs and practices utilizing the graded approach. Biennial review and additional reviews are conducted as significant programmatic and mission changes are made. This document is revised as necessary to maintain compliance with DOE requirements

340 Facility maintenance implementation plan

Energy Technology Data Exchange (ETDEWEB)

NONE

1995-03-01

This Maintenance Implementation Plan (MIP) has been developed for maintenance functions associated with the 340 Facility. This plan is developed from the guidelines presented by Department of Energy (DOE) Order 4330.4B, Maintenance Management Program (DOE 1994), Chapter II. The objective of this plan is to provide baseline information for establishing and identifying Westinghouse Hanford Company (WHC) conformance programs and policies applicable to implementation of DOE order 4330.4B guidelines. In addition, this maintenance plan identifies the actions necessary to develop a cost-effective and efficient maintenance program at the 340 Facility. Primary responsibility for the performance and oversight of maintenance activities at the 340 Facility resides with Westinghouse Hanford Company (WHC). Maintenance at the 340 Facility is performed by ICF-Kaiser Hanford (ICF-KH) South Programmatic Services crafts persons. This 340 Facility MIP provides interface requirements and responsibilities as they apply specifically to the 340 Facility. This document provides an implementation schedule which has been developed for items considered to be deficient or in need of improvement. The discussion sections, as applied to implementation at the 340 Facility, have been developed from a review of programs and practices utilizing the graded approach. Biennial review and additional reviews are conducted as significant programmatic and mission changes are made. This document is revised as necessary to maintain compliance with DOE requirements.

Reflooding phenomena of German PWR estimated from CCTF [Cylindrical Core Test Facility], SCTF [Slab Core Test Facility] and UPTF [Upper Plenum Test Facility] results

International Nuclear Information System (INIS)

Murao, Y.; Iguchi, T.; Sugimoto, J.

1988-09-01

The reflooding behavior in a PWR with a combined injection type ECCS was studied by comparing the test results from Cylindrical Core Test Facility (CCTF), Slab Core Test Facility (SCTF) and Upper Plenum Test Facility (UPTF). Core thermal-hydraulics is discussed mainly based on SCTF test data. In addition, the water accumulation behavior in hot legs and the break-through characteristics at tie plate are discussed

EPA Facility Registry Service (FRS): ER_FRP

Data.gov (United States)

U.S. Environmental Protection Agency — This dataset contains location and facility identification information from EPA's Facility Registry System (FRS) for the subset of facilities that link to Facility...

Hot cell verification facility update

International Nuclear Information System (INIS)

Titzler, P.A.; Moffett, S.D.; Lerch, R.E.

1985-01-01

The Hot Cell Verification Facility (HCVF) provides a prototypic hot cell mockup to check equipment for functional and remote operation, and provides actual hands-on training for operators. The facility arrangement is flexible and assists in solving potential problems in a nonradioactive environment. HCVF has been in operation for six years, and the facility is a part of the Hanford Engineering Development Laboratory

Future markets and technologies for natural gas vehicles; Futurs marches et technologies pour les vehicules au gaz naturel

Energy Technology Data Exchange (ETDEWEB)

Allen, J. [Development Engineer, Lotus Engineering (United Kingdom); Carpenter, B. [Gas Applications, BG Technology (United Kingdom)

2000-07-01

Lotus Engineering and BG Technology recently collaborated on the conversion of the Lotus Elise for operation on natural gas. This paper considers the world-wide opportunities for natural gas as an automotive fuel by comparison with other fuels. It looks at how technology can be used to exploit this potential, by examining the special features of the gas fuelled Elise, and how other technologies such as hybrid vehicles and fuel cells can be expected to respond to this challenge in future. (authors)

Improvement of irradiation facilities performance in JMTR

International Nuclear Information System (INIS)

Kanno, Masaru; Sakurai, Susumu; Honma, Kenzo; Sagawa, Hisashi; Nakazaki, Chousaburo

1999-01-01

Various kinds of irradiation facilities are installed in the JMTR for the purpose of irradiation tests on fuels and materials and of producing radioisotopes. The irradiation facilities have been improved so far at every opportunity of new irradiation requirements and of renewing them which reached the design lifetime. Of these irradiation facilities, improvements of the power ramping test facility (BOCA/OSF-1 facility) and the hydraulic rabbit No.2 (HR-2 facility) are described here. (author)

Safety of nuclear fuel cycle facilities. Safety requirements

International Nuclear Information System (INIS)

2008-01-01

This publication covers the broad scope of requirements for fuel cycle facilities that, in light of the experience and present state of technology, must be satisfied to ensure safety for the lifetime of the facility. Topics of specific reference include aspects of nuclear fuel generation, storage, reprocessing and disposal. Contents: 1. Introduction; 2. The safety objective, concepts and safety principles; 3. Legal framework and regulatory supervision; 4. The management system and verification of safety; 5. Siting of the facility; 6. Design of the facility; 7. Construction of the facility; 8. Commissioning of the facility; 9. Operation of the facility; 10. Decommissioning of the facility; Appendix I: Requirements specific to uranium fuel fabrication facilities; Appendix II: Requirements specific to mixed oxide fuel fabrication facilities; Appendix III: Requirements specific to conversion facilities and enrichment facilities

TREAT neutron-radiography facility

International Nuclear Information System (INIS)

Harrison, L.J.

1981-01-01

The TREAT reactor was built as a transient irradiation test reactor. By taking advantage of built-in system features, it was possible to add a neutron-radiography facility. This facility has been used over the years to radiograph a wide variety and large number of preirradiated fuel pins in many different configurations. Eight different specimen handling casks weighing up to 54.4 t (60 T) can be accommodated. Thermal, epithermal, and track-etch radiographs have been taken. Neutron-radiography service can be provided for specimens from other reactor facilities, and the capacity for storing preirradiated specimens also exists

Classified facilities for environmental protection

International Nuclear Information System (INIS)

Anon.

1993-02-01

The legislation of the classified facilities governs most of the dangerous or polluting industries or fixed activities. It rests on the law of 9 July 1976 concerning facilities classified for environmental protection and its application decree of 21 September 1977. This legislation, the general texts of which appear in this volume 1, aims to prevent all the risks and the harmful effects coming from an installation (air, water or soil pollutions, wastes, even aesthetic breaches). The polluting or dangerous activities are defined in a list called nomenclature which subjects the facilities to a declaration or an authorization procedure. The authorization is delivered by the prefect at the end of an open and contradictory procedure after a public survey. In addition, the facilities can be subjected to technical regulations fixed by the Environment Minister (volume 2) or by the prefect for facilities subjected to declaration (volume 3). (A.B.)

Fuel Handling Facility Description Document

International Nuclear Information System (INIS)

M.A. LaFountain

2005-01-01

The purpose of the facility description document (FDD) is to establish the requirements and their bases that drive the design of the Fuel Handling Facility (FHF) to allow the design effort to proceed to license application. This FDD is a living document that will be revised at strategic points as the design matures. It identifies the requirements and describes the facility design as it currently exists, with emphasis on design attributes provided to meet the requirements. This FDD was developed as an engineering tool for design control. Accordingly, the primary audience and users are design engineers. It leads the design process with regard to the flow down of upper tier requirements onto the facility. Knowledge of these requirements is essential to performing the design process. It trails the design with regard to the description of the facility. This description is a reflection of the results of the design process to date

Strategic facility planning improves capital decision making.

Science.gov (United States)

Reeve, J R

2001-03-01

A large, Midwestern IDS undertook a strategic facility-planning process to evaluate its facility portfolio and determine how best to allocate future investments in facility development. The IDS assembled a facility-planning team, which initiated the planning process with a market analysis to determine future market demands and identify service areas that warranted facility expansion. The team then analyzed each of the IDS's facilities from the perspective of uniform capacity measurements, highest and best use compared with needs, building condition and investment-worthiness, and facility growth and site development opportunities. Based on results of the analysis, the strategy adopted entailed, in part, shifting some space from inpatient care to ambulatory care services and demolishing and replacing the 11 percent of facilities deemed to be in the worst condition.

Facility or Facilities? That is the Question.

Science.gov (United States)

Viso, M.

2018-04-01

The management of the martian samples upon arrival on the Earth will require a lot of work to ensure a safe life detection and biohazard testing during the quarantine. This will induce a sharing of the load between several facilities.

Eccentric Coil Test Facility (ECTF)

International Nuclear Information System (INIS)

Burn, P.B.; Walstrom, P.L.; Anderson, W.C.; Marguerat, E.F.

1975-01-01

The conceptual design of a facility for testing superconducting coils under some conditions peculiar to tokamak systems is given. A primary element of the proposed facility is a large 25 MJ background solenoid. Discussions of the mechanical structure, the stress distribution and the thermal stability for this coil are included. The systems for controlling the facility and diagnosing test coil behavior are also described

Facility effluent monitoring plan for the Waste Receiving and Processing Facility Module 1

International Nuclear Information System (INIS)

Lewis, C.J.

1995-10-01

A facility effluent monitoring plan is required by the US Department of Energy in Order 5400.1 for any operations that involve hazardous materials and radioactive substances that could impact employee or public safety or the environment. This document is prepared using the specific guidelines identified in A Guide for Preparing Hanford Site Facility Effluent Monitoring Plans, WHC-EP-0438. This facility effluent monitoring plan assesses effluent monitoring systems and evaluates whether they are adequate to ensure the public health and safety as specified in applicable federal state, and local requirements. This facility effluent monitoring plan shall ensure lonq-range integrity of the effluent monitoring systems by requiring an update whenever a new process or operation introduces new hazardous materials or significant radioactive materials. This document must be reviewed annually even if there are no operational changes, and it must be updated as a minimum every three years

Evaluation of existing United States' facilities for use as a mixed-oxide (MOX) fuel fabrication facility for plutonium disposition

International Nuclear Information System (INIS)

Beard, C.A.; Buksa, J.J.; Chidester, K.; Eaton, S.L.; Motley, F.E.; Siebe, D.A.

1995-01-01

A number of existing US facilities were evaluated for use as a mixed-oxide fuel fabrication facility for plutonium disposition. These facilities include the Fuels Material Examination Facility (FMEF) at Hanford, the Washington Power Supply Unit 1 (WNP-1) facility at Hanford, the Barnwell Nuclear Fuel Plant (BNFP) at Barnwell, SC, the Fuel Processing Facility (FPF) at Idaho National Engineering Laboratory (INEL), the Device Assembly Facility (DAF) at the Nevada Test Site (NTS), and the P-reactor at the Savannah River Site (SRS). The study consisted of evaluating each facility in terms of available process space, available building support systems (i.e., HVAC, security systems, existing process equipment, etc.), available regional infrastructure (i.e., emergency response teams, protective force teams, available transportation routes, etc.), and ability to integrate the MOX fabrication process into the facility in an operationally-sound manner that requires a minimum amount of structural modifications

Fabrication of Hierarchically Micro- and Nano-structured Mold Surfaces Using Laser Ablation for Mass Production of Superhydrophobic Surfaces

Science.gov (United States)

Noh, Jiwhan; Lee, Jae-Hoon; Na, Suckjoo; Lim, Hyuneui; Jung, Dae-Hwan

2010-10-01

Many studies have examined the formation of surfaces with mixed patterns of micro- and nano-sized lotus leaves that have hydrophobic properties. In this study, micro- and nano-shapes such as lotus leaves were fabricated on a metal mold surface using laser ablation and ripple formation. A microstructure on the mold surface was replicated onto poly(dimethylsiloxane) (PDMS) using the polymer casting method to manufacture low-cost hydrophobic surfaces. A PDMS surface with micro- and nano-structures that were the inverse image of a lotus leaf showed hydrophobic characteristics (water contact angle: 157°). From these results, we deduced that portions of the microstructures were wet and that air gaps existed between the microstructures and the water drops. In this paper we suggest the possibility of the mass production of hydrophobic plastic surfaces and the development of a methodology for the hydrophobic texturing of various polymer surfaces, using the polymer casting method with laser-processed molds.

Assessment of Nelumbo nucifera and Hydrilla verticillata in the treatment of pharmaceutical industry effluent from 24 Parganas, West Bengal

Directory of Open Access Journals (Sweden)

Shamba Chatterjee

2014-10-01

Full Text Available Modern day technologies employed in industrialization and unhygienic lifestyle of mankind has led to a severe environmental menace resulting in pollution of freshwater bodies. Pharmaceutical industry effluents cause eutrophication and provide adequate nutrients for growth of pathogenic bacteria. This study has been conducted with aquatic plants water lotus (Nelumbo nucifera and hydrilla (Hydrilla verticillata with an novel aim to treat pharmaceutical industry effluents showing the outcome of the experiments carried out with the effluents collected from rural areas of 24 Parganas, West Bengal, India. Determination of pH, solid suspend, BOD5, NH3-N, MPN and coliform test were used for this notioned purpose. Pharmaceutical waste effluent water treated with water lotus showed less pH, solid suspend, DO, BOD, NH3-N, MPN and coliform bacteria than hydrilla treatment when compared to the control. In conclusion, water lotus is found to be more efficient in treatment of pharmaceutical industry effluent waste water than hydrilla.

Hazard Baseline Downgrade Effluent Treatment Facility

International Nuclear Information System (INIS)

Blanchard, A.

1998-01-01

This Hazard Baseline Downgrade reviews the Effluent Treatment Facility, in accordance with Department of Energy Order 5480.23, WSRC11Q Facility Safety Document Manual, DOE-STD-1027-92, and DOE-EM-STD-5502-94. It provides a baseline grouping based on the chemical and radiological hazards associated with the facility. The Determination of the baseline grouping for ETF will aid in establishing the appropriate set of standards for the facility

Sports Facilities Development and Urban Generation

OpenAIRE

Maassoumeh Barghchi; Dasimah B.   Omar; Mohd S.   Aman

2009-01-01

Problem statement: One major issue on sports facilities construction is the question of their funding and justification for investment. Due to, requirement of huge money for construction, constant maintenance costs and ancillary needs, which are almost certainly with substantial public investment, therefore, sports facilities have been considered. Further, sports facilities construction boom have been started for more than two decades. Approach: Recent sports facilities construction was not p...

HNF - Helmholtz Nano Facility

Directory of Open Access Journals (Sweden)

Wolfgang Albrecht

2017-05-01

Full Text Available The Helmholtz Nano Facility (HNF is a state-of-the-art cleanroom facility. The cleanroom has ~1100 m2 with cleanroom classes of DIN ISO 1-3. HNF operates according to VDI DIN 2083, Good Manufacturing Practice (GMP and aquivalent to Semiconductor Industry Association (SIA standards. HNF is a user facility of Forschungszentrum Jülich and comprises a network of facilities, processes and systems for research, production and characterization of micro- and nanostructures. HNF meets the basic supply of micro- and nanostructures for nanoelectronics, fluidics. micromechanics, biology, neutron and energy science, etc.. The task of HNF is rapid progress in nanostructures and their technology, offering efficient access to infrastructure and equipment. HNF gives access to expertise and provides resources in production, synthesis, characterization and integration of structures, devices and circuits. HNF covers the range from basic research to application oriented research facilitating a broad variety of different materials and different sample sizes.

Current status of collaborative relationships between dialysis facilities and dental facilities in Japan: results of a nationwide survey.

Science.gov (United States)

Yoshioka, Masami; Shirayama, Yasuhiko; Imoto, Issei; Hinode, Daisuke; Yanagisawa, Shizuko; Takeuchi, Yuko

2015-02-12

Recent studies have reported an association between periodontal disease and mortality among dialysis patients. Therefore, preventive dental care should be considered very important for this population. In Japan, no systematic education has been undertaken regarding the importance of preventive dental care for hemodialysis patients--even though these individuals tend to have oral and dental problems. The aim of this study was to investigate the current state of collaborative relationships between hemodialysis facilities and dental services in Japan and also to identify strategies to encourage preventive dental visits among hemodialysis outpatients. A nationwide questionnaire on the collaborative relationship between dialysis facilities and dental facilities was sent by mail to all medical facilities in Japan offering outpatient hemodialysis treatment. Responses were obtained from 1414 of 4014 facilities (35.2%). Among the 1414 facilities, 272 (19.2%) had a dental service department. Approximately 100,000 dialysis outpatients were receiving treatment at these participating facilities, which amounts to one-third of all dialysis patients in Japan. Of those patients, 82.9% received hemodialysis at medical facilities without dental departments. Only 87 of 454 small clinics without in-house dental departments (19.2%) had collaborative registered dental clinics. Medical facilities with registered dental clinics demonstrated a significantly more proactive attitude to routine collaboration on dental matters than facilities lacking such clinics. Our nationwide survey revealed that most dialysis facilities in Japan have neither an in-house dental department nor a collaborative relationship with a registered dental clinic. Registration of dental clinics appears to promote collaboration with dental facilities on a routine basis, which would be beneficial for oral health management in hemodialysis patients.

30 CFR 71.402 - Minimum requirements for bathing facilities, change rooms, and sanitary flush toilet facilities.

Science.gov (United States)

2010-07-01

... nonirritating cleansing agent shall be provided for use at each shower. (2) Sanitary flush toilet facilities. (i..., change rooms, and sanitary flush toilet facilities. 71.402 Section 71.402 Mineral Resources MINE SAFETY... Rooms, and Sanitary Flush Toilet Facilities at Surface Coal Mines § 71.402 Minimum requirements for...

Initial operation of the Holifield facility

International Nuclear Information System (INIS)

Ball, J.B.

1982-01-01

The Holifield Heavy Ion Research Facility (HHIRF) is located at Oak Ridge National Laboratory and operated, by the Physics Division, as a national user facility for research in heavy-ion science. The facility operates two accelerators: the new Pelletron electrostatic accelerator, designed to accelerate all ions at terminal potentials up to 25 million volts, and the Oak Ridge Isochronous Cyclotron (ORIC) which, in addition to its stand-alone capabilities, has been modified to serve also as a booster accelerator for ion beams from the Pelletron. In addition, a number of state-of-the-art experimental devices, a new data acquisition computer system, and special user accommodations have been implemented as part of the facility. The construction of the facility was completed officially in June of this year. This paper reports on the present status of facility operation, observations from testing and running of the 25 MV Pelletron, experience with coupled operation of the Pelletron with the ORIC booster, and a brief summary of the experimental devices now available at the facility

Initial operation of the Holifield Facility

International Nuclear Information System (INIS)

Ball, J.B.

1983-01-01

The Holifield Heavy Ion Research Facility (HHIRF) is located at Oak Ridge National Laboratory and operated, by the Physics Division, as a national user facility for research in heavy-ion science. The facility operates two accelerators: the new pelletron electrostatic accelerator, designed to accelerate all ions at terminal potentials up to 25 million volts, and the Oak Ridge Isochronous Cyclotron (ORIC) which, in addition to its stand-alone capabilities, has been modified to serve also as a booster accelerator for ion beams from the Pelletron. In addition, a number of state-of-the-art experimental devices, a new data acquisition computer system, and special user accommodations have been implemented as part of the facility. The construction of the facility was completed officially in June of this year. This paper reports on the present status of facility operation, observations from testing and running of the 25 MV Pelletron, experience with coupled operation of the Pelletron with the ORIC booster, and a brief summary of the experimental devices now available at the facility

Scaling laws between population and facility densities.

Science.gov (United States)

Um, Jaegon; Son, Seung-Woo; Lee, Sung-Ik; Jeong, Hawoong; Kim, Beom Jun

2009-08-25

When a new facility like a grocery store, a school, or a fire station is planned, its location should ideally be determined by the necessities of people who live nearby. Empirically, it has been found that there exists a positive correlation between facility and population densities. In the present work, we investigate the ideal relation between the population and the facility densities within the framework of an economic mechanism governing microdynamics. In previous studies based on the global optimization of facility positions in minimizing the overall travel distance between people and facilities, it was shown that the density of facility D and that of population rho should follow a simple power law D approximately rho(2/3). In our empirical analysis, on the other hand, the power-law exponent alpha in D approximately rho(alpha) is not a fixed value but spreads in a broad range depending on facility types. To explain this discrepancy in alpha, we propose a model based on economic mechanisms that mimic the competitive balance between the profit of the facilities and the social opportunity cost for populations. Through our simple, microscopically driven model, we show that commercial facilities driven by the profit of the facilities have alpha = 1, whereas public facilities driven by the social opportunity cost have alpha = 2/3. We simulate this model to find the optimal positions of facilities on a real U.S. map and show that the results are consistent with the empirical data.

National Biomedical Tracer Facility: Project definition study

International Nuclear Information System (INIS)

Heaton, R.; Peterson, E.; Smith, P.

1995-01-01

The Los Alamos National Laboratory is an ideal institution and New Mexico is an ideal location for siting the National Biomedical Tracer Facility (NBTF). The essence of the Los Alamos proposal is the development of two complementary irradiation facilities that combined with our existing radiochemical processing hot cell facilities and waste handling and disposal facilities provide a low cost alternative to other proposals that seek to satisfy the objectives of the NBTF. We propose the construction of a 30 MeV cyclotron facility at the site of the radiochemical facilities, and the construction of a 100 MeV target station at LAMPF to satisfy the requirements and objectives of the NBTF. We do not require any modifications to our existing radiochemical processing hot cell facilities or our waste treatment and disposal facilities to accomplish the objectives of the NBTF. The total capital cost for the facility defined by the project definition study is $15.2 M. This cost estimate includes $9.9 M for the cyclotron and associated facility, $2.0 M for the 100 MeV target station at LAMPF, and $3.3 M for design