Lin Receives 2010 Natural Hazards Focus Group Award for Graduate Research

Science.gov (United States)

2010-11-01

Ning Lin has been awarded the Natural Hazards Focus Group Award for Graduate Research, given annually to a recent Ph.D. recipient for outstanding contributions to natural hazards research. Lin's thesis is entitled “Multi-hazard risk analysis related to hurricanes.” She is scheduled to present an invited talk in the Extreme Natural Events: Modeling, Prediction, and Mitigation session (NH20) during the 2010 AGU Fall Meeting, held 13-17 December in San Francisco, Calif. Lin will be formally presented with the award at the Natural Hazards focus group reception on 14 December 2010.

Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models

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Nguyen, Liem H.; Robinton, Daisy A.; Seligson, Marc; Wu, Linwei; Li, Lin; Rakheja, Dinesh; Comerford, Sarah; Ramezani, Saleh; Sun, Xiankai; Parikh, Monisha; Yang, Erin; Powers, John T.; Shinoda, Gen; Shah, Samar; Hammer, Robert; Daley, George Q.; Zhu, Hao

2014-01-01

SUMMARY Lin28a/b are RNA-binding proteins that influence stem cell maintenance, metabolism, and oncogenesis. Poorly differentiated, aggressive cancers often overexpress Lin28, but its role in tumor initiation or maintenance has not been definitively addressed. We report that LIN28B overexpression is sufficient to initiate hepatoblastoma and hepatocellular carcinoma in murine models. We also detected Lin28b overexpression in MYC-driven hepatoblastomas, and liver-specific deletion of Lin28a/b reduced tumor burden, extended latency, and prolonged survival. Both intravenous siRNA against Lin28b and conditional Lin28b deletion reduced tumor burden and prolonged survival. Igf2bp proteins are upregulated and Igf2bp3 is required in the context of LIN28B overexpression to promote growth. Thus, multiple murine models demonstrate that Lin28b is both sufficient to initiate liver cancer and necessary for its maintenance. PMID:25117712

Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers.

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Pooja Sharma

Full Text Available BACKGROUND: Here we report specific activities of all seven naturally occurring LinA variants towards three different isomers, α, γ and δ, of a priority persistent pollutant, hexachlorocyclohexane (HCH. Sequence-structure-function differences contributing to the differences in their stereospecificity for α-, γ-, and δ-HCH and enantiospecificity for (+- and (--α -HCH are also discussed. METHODOLOGY/PRINCIPAL FINDINGS: Enzyme kinetic studies were performed with purified LinA variants. Models of LinA2(B90A A110T, A111C, A110T/A111C and LinA1(B90A were constructed using the FoldX computer algorithm. Turnover rates (min(-1 showed that the LinAs exhibited differential substrate affinity amongst the four HCH isomers tested. α-HCH was found to be the most preferred substrate by all LinA's, followed by the γ and then δ isomer. CONCLUSIONS/SIGNIFICANCE: The kinetic observations suggest that LinA-γ1-7 is the best variant for developing an enzyme-based bioremediation technology for HCH. The majority of the sequence variation in the various linA genes that have been isolated is not neutral, but alters the enantio- and stereoselectivity of the encoded proteins.

Lin4Neuro: a customized Linux distribution ready for neuroimaging analysis.

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Nemoto, Kiyotaka; Dan, Ippeita; Rorden, Christopher; Ohnishi, Takashi; Tsuzuki, Daisuke; Okamoto, Masako; Yamashita, Fumio; Asada, Takashi

2011-01-25

A variety of neuroimaging software packages have been released from various laboratories worldwide, and many researchers use these packages in combination. Though most of these software packages are freely available, some people find them difficult to install and configure because they are mostly based on UNIX-like operating systems. We developed a live USB-bootable Linux package named "Lin4Neuro." This system includes popular neuroimaging analysis tools. The user interface is customized so that even Windows users can use it intuitively. The boot time of this system was only around 40 seconds. We performed a benchmark test of inhomogeneity correction on 10 subjects of three-dimensional T1-weighted MRI scans. The processing speed of USB-booted Lin4Neuro was as fast as that of the package installed on the hard disk drive. We also installed Lin4Neuro on a virtualization software package that emulates the Linux environment on a Windows-based operation system. Although the processing speed was slower than that under other conditions, it remained comparable. With Lin4Neuro in one's hand, one can access neuroimaging software packages easily, and immediately focus on analyzing data. Lin4Neuro can be a good primer for beginners of neuroimaging analysis or students who are interested in neuroimaging analysis. It also provides a practical means of sharing analysis environments across sites.

Lin28 Mediates Cancer Chemotherapy Resistance via Regulation of miRNA Signaling.

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Xu, Chaoyang; Xie, Shuduo; Song, Chunjiao; Huang, Liming; Jiang, Zhinong

2014-06-01

Chemotherapy resistance is one of the major obstacles limiting the success of cancer drug treatment. Among the mechanisms of resistance to chemotherapy treatment, there are those closely related to P-Glycoprotein, multidrug resistance-related protein, glutathione S-transferase pi and topoisomerase-II. Lin28 is a highly conserved RNA-binding protein, it consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs. In previous preclinical and clinical studies, positive Lin28 expression in cancer cells was correlated with decreased sensitivity to chemotherapy. And Lin28 could mediate cancer chemotherapy resistance via regulation of miR107 and Let-7 MiRNA. This article reviews current knowledge on predictive value of Lin28 in response to chemotherapy. Better understanding of its role may facilitate patient's selection of therapeutic regimen and lead to optimal clinical outcome.

Lin28b stimulates the reprogramming of rat Müller glia to retinal progenitors

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Zhao, Chen; Tao, Zui; Xue, Langyue; Zeng, Yuxiao [Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038 (China); Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038 (China); Wang, Yi, E-mail: wangyieye@aliyun.com [Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038 (China); Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038 (China); Xu, Haiwei, E-mail: haiweixu2001@163.com [Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038 (China); Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038 (China); Yin, Zheng Qin, E-mail: qinzyin@aliyun.com [Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038 (China); Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038 (China)

2017-03-01

In lower-order vertebrates, Müller glia exhibit characteristics of retinal progenitor cells, while in higher vertebrates, such as mammals, the regenerative capacity of Müller glia is limited. Recently, we reported that Lin28b promoted the trans-differentiation of Müller cells to rod photoreceptor and bipolar cells in the retina of retinitis pigmentosa rat model, whereas it is unclear whether Lin28b can stimulate the reprogramming of Müller glia in vitro for transplantation into a damaged retina. In the present study, Long-Evens rat Müller glia were infected with Adeno-Lin28b or Adeno-GFP. Over-expression of Lin28b in isolated rat Müller glia resulted in the suppression of GFAP expression, enhancement of cell proliferation and a significant increase of the expression of retinal progenitor markers 5 days after infection. Moreover, Lin28b caused a significant reduction of the Let-7 family of microRNAs. Following sub-retinal space transplantation, Müller glia-derived retinal progenitors improved b-wave amplification of 30d Royal College of Surgeons retinitis pigmentosa model (RCS-P+) rats, as detected by electroretinography (ERG) recordings. Taken together, these data suggest that the up-regulation of Lin28b expression facilitated the reprogramming of Müller cells toward characteristics of retinal progenitors. - Highlights: • Lin28b reprograms Müller glia to retinal progenitors. • Let-7 micrRNAs are suppressed by Lin28b. • Transplantation of reprogrammed Müller glia restores retinal function.

Lin28b stimulates the reprogramming of rat Müller glia to retinal progenitors

International Nuclear Information System (INIS)

Zhao, Chen; Tao, Zui; Xue, Langyue; Zeng, Yuxiao; Wang, Yi; Xu, Haiwei; Yin, Zheng Qin

2017-01-01

In lower-order vertebrates, Müller glia exhibit characteristics of retinal progenitor cells, while in higher vertebrates, such as mammals, the regenerative capacity of Müller glia is limited. Recently, we reported that Lin28b promoted the trans-differentiation of Müller cells to rod photoreceptor and bipolar cells in the retina of retinitis pigmentosa rat model, whereas it is unclear whether Lin28b can stimulate the reprogramming of Müller glia in vitro for transplantation into a damaged retina. In the present study, Long-Evens rat Müller glia were infected with Adeno-Lin28b or Adeno-GFP. Over-expression of Lin28b in isolated rat Müller glia resulted in the suppression of GFAP expression, enhancement of cell proliferation and a significant increase of the expression of retinal progenitor markers 5 days after infection. Moreover, Lin28b caused a significant reduction of the Let-7 family of microRNAs. Following sub-retinal space transplantation, Müller glia-derived retinal progenitors improved b-wave amplification of 30d Royal College of Surgeons retinitis pigmentosa model (RCS-P+) rats, as detected by electroretinography (ERG) recordings. Taken together, these data suggest that the up-regulation of Lin28b expression facilitated the reprogramming of Müller cells toward characteristics of retinal progenitors. - Highlights: • Lin28b reprograms Müller glia to retinal progenitors. • Let-7 micrRNAs are suppressed by Lin28b. • Transplantation of reprogrammed Müller glia restores retinal function.

Importance of the pluripotency factor LIN28 in the mammalian nucleolus during early embryonic development.

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Vogt, Edgar J; Meglicki, Maciej; Hartung, Kristina Ilka; Borsuk, Ewa; Behr, Rüdiger

2012-12-01

The maternal nucleolus is required for proper activation of the embryonic genome (EGA) and early embryonic development. Nucleologenesis is characterized by the transformation of a nucleolar precursor body (NPB) to a mature nucleolus during preimplantation development. However, the function of NPBs and the involved molecular factors are unknown. We uncover a novel role for the pluripotency factor LIN28, the biological significance of which was previously demonstrated in the reprogramming of human somatic cells to induced pluripotent stem (iPS) cells. Here, we show that LIN28 accumulates at the NPB and the mature nucleolus in mouse preimplantation embryos and embryonic stem cells (ESCs), where it colocalizes with the nucleolar marker B23 (nucleophosmin 1). LIN28 has nucleolar localization in non-human primate (NHP) preimplantation embryos, but is cytoplasmic in NHP ESCs. Lin28 transcripts show a striking decline before mouse EGA, whereas LIN28 protein localizes to NPBs at the time of EGA. Following knockdown with a Lin28 morpholino, the majority of embryos arrest between the 2- and 4-cell stages and never develop to morula or blastocyst. Lin28 morpholino-injected embryos arrested at the 2-cell stage were not enriched with nucleophosmin at presumptive NPB sites, indicating that functional NPBs were not assembled. Based on these results, we propose that LIN28 is an essential factor of nucleologenesis during early embryonic development.

LIN28 phosphorylation by MAPK/ERK couples signaling to the post-transcriptional control of pluripotency

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Tsanov, Kaloyan M.; Pearson, Daniel S.; Wu, Zhaoting; Han, Areum; Triboulet, Robinson; Seligson, Marc T.; Powers, John T.; Osborne, Jihan K.; Kane, Susan; Gygi, Steven P.; Gregory, Richard I.; Daley, George Q.

2016-01-01

Signaling and post-transcriptional gene control are both critical for the regulation of pluripotency1,2, yet how they are integrated to influence cell identity remains poorly understood. LIN28 (also known as LIN28A), a highly conserved RNA-binding protein (RBP), has emerged as a central post-transcriptional regulator of cell fate through blockade of let-7 microRNA (miRNA) biogenesis and direct modulation of mRNA translation3. Here we show that LIN28 is phosphorylated by MAPK/ERK in pluripotent stem cells (PSCs), which increases its levels via post-translational stabilization. LIN28 phosphorylation had little impact on let-7 but enhanced LIN28’s effect on its direct mRNA targets, revealing a mechanism that uncouples LIN28’s let-7-dependent and independent activities. We have linked this mechanism to the induction of pluripotency by somatic cell reprogramming and the transition from naïve to primed pluripotency. Collectively, our findings indicate that MAPK/ERK directly impacts LIN28, defining an axis that connects signaling, post-transcriptional gene control, and cell fate regulation. PMID:27992407

A Dynamic Attitude Measurement System Based on LINS

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Hanzhou Li

2014-08-01

Full Text Available A dynamic attitude measurement system (DAMS is developed based on a laser inertial navigation system (LINS. Three factors of the dynamic attitude measurement error using LINS are analyzed: dynamic error, time synchronization and phase lag. An optimal coning errors compensation algorithm is used to reduce coning errors, and two-axis wobbling verification experiments are presented in the paper. The tests indicate that the attitude accuracy is improved 2-fold by the algorithm. In order to decrease coning errors further, the attitude updating frequency is improved from 200 Hz to 2000 Hz. At the same time, a novel finite impulse response (FIR filter with three notches is designed to filter the dither frequency of the ring laser gyro (RLG. The comparison tests suggest that the new filter is five times more effective than the old one. The paper indicates that phase-frequency characteristics of FIR filter and first-order holder of navigation computer constitute the main sources of phase lag in LINS. A formula to calculate the LINS attitude phase lag is introduced in the paper. The expressions of dynamic attitude errors induced by phase lag are derived. The paper proposes a novel synchronization mechanism that is able to simultaneously solve the problems of dynamic test synchronization and phase compensation. A single-axis turntable and a laser interferometer are applied to verify the synchronization mechanism. The experiments results show that the theoretically calculated values of phase lag and attitude error induced by phase lag can both match perfectly with testing data. The block diagram of DAMS and physical photos are presented in the paper. The final experiments demonstrate that the real-time attitude measurement accuracy of DAMS can reach up to 20″ (1σ and the synchronization error is less than 0.2 ms on the condition of three axes wobbling for 10 min.

A Dynamic Attitude Measurement System Based on LINS

Science.gov (United States)

Li, Hanzhou; Pan, Quan; Wang, Xiaoxu; Zhang, Juanni; Li, Jiang; Jiang, Xiangjun

2014-01-01

A dynamic attitude measurement system (DAMS) is developed based on a laser inertial navigation system (LINS). Three factors of the dynamic attitude measurement error using LINS are analyzed: dynamic error, time synchronization and phase lag. An optimal coning errors compensation algorithm is used to reduce coning errors, and two-axis wobbling verification experiments are presented in the paper. The tests indicate that the attitude accuracy is improved 2-fold by the algorithm. In order to decrease coning errors further, the attitude updating frequency is improved from 200 Hz to 2000 Hz. At the same time, a novel finite impulse response (FIR) filter with three notches is designed to filter the dither frequency of the ring laser gyro (RLG). The comparison tests suggest that the new filter is five times more effective than the old one. The paper indicates that phase-frequency characteristics of FIR filter and first-order holder of navigation computer constitute the main sources of phase lag in LINS. A formula to calculate the LINS attitude phase lag is introduced in the paper. The expressions of dynamic attitude errors induced by phase lag are derived. The paper proposes a novel synchronization mechanism that is able to simultaneously solve the problems of dynamic test synchronization and phase compensation. A single-axis turntable and a laser interferometer are applied to verify the synchronization mechanism. The experiments results show that the theoretically calculated values of phase lag and attitude error induced by phase lag can both match perfectly with testing data. The block diagram of DAMS and physical photos are presented in the paper. The final experiments demonstrate that the real-time attitude measurement accuracy of DAMS can reach up to 20″ (1σ) and the synchronization error is less than 0.2 ms on the condition of three axes wobbling for 10 min. PMID:25177802

Le nævus épidermique verruqueux inflammatoire linéaire ...

African Journals Online (AJOL)

Le nævus épidermique verruqueux inflammatoire linéaire (NEVIL) est une affection rare correspondant à des hyperplasies épidermiques bénignes qui se présentent cliniquement sous forme de lésions linéaires unilatérales hyperkératosiques. Une adolescente âgée de 16 ans, sans antécédents, présentait depuis la ...

107 Définition structurale des linéaments par traitement d'image ...

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The Ngovayang massif represents a key domain in understanding the geodynamics of the northern border of the Congo craton in South Cameroon. Remote sensing works .... linéaments liés au socle rocheux et aux accidents tectoniques (Minyem, 1994 [2]). Ces linéaments sont repartis selon trois directions. - la direction ...

Back-propagation neural network in tidal-level forecasting by Ching-PiaoTsai and Tsong-Lin Lee - Discussion

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Mandal, S.

relevance to the practicing engineer. Such a scheme of updating parameters (connection weights in the case of neu- ral network) is not employed in the suggested ANN model; thus, the ANN model, where the parameters are estimated from only one-day data... term hourly tidal records. However, there are a number of points to be clarified in order to better understand the paper. This discussion focuses on the following four areas: (1) terminology used in the paper; (2) practical utility; (3) reliability...

LIN-32/Atonal Controls Oxygen Sensing Neuron Development in Caenorhabditis elegans

DEFF Research Database (Denmark)

Romanos, Teresa Rojo; Pladevall-Morera, David; Langebeck-Jensen, Kasper

2017-01-01

HLH) family of transcription factors has multiple functions in neurogenesis. Here, we identified the LIN-32/Atonal bHLH transcription factor as a key regulator of URXL/R oxygen-sensing neuron development in Caenorhabditis elegans. When LIN-32/Atonal expression is lost, the expression of URX specification......Development of complex nervous systems requires precisely controlled neurogenesis. The generation and specification of neurons occur through the transcriptional and post-Transcriptional control of complex regulatory networks. In vertebrates and invertebrates, the proneural basic-helix-loop-helix (b...... and terminal differentiation genes is abrogated. As such, lin-32 mutant animals are unable to respond to increases in environmental oxygen. The URX neurons are generated from a branch of the cell lineage that also produces the CEPDL/R and URADL/R neurons. We found development of these neurons is also defective...

Regulation of C. elegans L4 cuticle collagen genes by the heterochronic protein LIN-29.

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Abete-Luzi, Patricia; Eisenmann, David M

2018-05-01

The cuticle, the outer covering of the nematode C. elegans, is synthesized five times during the worm's life by the underlying hypodermis. Cuticle collagens, the major cuticle component, are encoded by a large family of col genes and, interestingly, many of these genes express predominantly at a single developmental stage. This temporal preference motivated us to investigate the mechanisms underlying col gene expression and here we focus on a subset of col genes expressed in the L4 stage. We identified minimal promoter regions of <300 bp for col-38, col-49, and col-63. In these regions, we predicted cis-regulatory sequences and evaluated their function in vivo via mutagenesis of a col-38p::yfp reporter. We used RNAi to study the requirement for candidate transcription regulators ELT-1 and ELT-3, LIN-29, and the LIN-29 co-factor MAB-10, and found LIN-29 to be necessary for the expression of four L4-specific genes (col-38, col-49, col-63, and col-138). Temporal misexpression of LIN-29 was also sufficient to activate these genes at a different developmental stage. The LIN-29 DNA-binding domain bound the col-38, col-49, and col-63 minimal promoters in vitro. For col-38 we showed that the LIN-29 sites necessary for reporter expression in vivo are also bound in vitro: this is the first identification of specific binding sites for LIN-29 necessary for in vivo target gene expression. © 2018 Wiley Periodicals, Inc.

régression linéaire multiple

African Journals Online (AJOL)

Mots clés: Alcools et phénols – Représentation numérique de la structure chimique – Facteur acentrique – Régression linéaire multiple – Modèle RSP hybride. English Title: Structure / acentric factor relationship of alcohols and phenols: genetic algorithm – multiple linear regression approach. English Abstract. The acentric ...

Association of Lin-28A rs3811464 Variant with Susceptibility to Type 2 Diabetes

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Mona Khodabandeh

2017-11-01

Full Text Available Introduction: It has been suggested that Lin-28A and the let-7 microRNA family (Lin-28/let-7 axis play a critical role in the control of glucose metabolism, insulin sensitivity and resistance to diabetes. Aim: This case-control study aimed at evaluating the association between Lin-28 rs3811464 polymorphism and the susceptibility to Type 2 Diabetes (T2D in a sample of Iranian population. Materials and Methods: This study involved 172 T2D patients and 160 non-diabetic age and gender-matched controls. Lin 28A rs3811464 genotypes were determined by Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP technique. Results: The results showed that the frequency of the AA genotype was significantly higher in control subjects than in diabetic patients (13.12% vs. 4.65%. In addition, binary logistic regression analysis revealed that rs3811464-AA genotype was significantly associated to T2D after adjustment for BMI, age and lipid profiles. Indeed, subjects with AA genotype were less likely to develop T2D than GG and AG subjects (OR of 0.26, 95% CI 0.10-0.66, p=0.005. Conclusion: The findings of our study suggest that the Lin 28A rs3811464 is associated with type 2 diabetes susceptibility and subjects with AA genotypes were less likely to develop T2D diabetes.

Metabolomics of hexachlorocyclohexane (HCH) transformation: ratio of LinA to LinB determines metabolic fate of HCH isomers.

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Geueke, Birgit; Garg, Nidhi; Ghosh, Sneha; Fleischmann, Thomas; Holliger, Christof; Lal, Rup; Kohler, Hans-Peter E

2013-04-01

Although the production and use of technical hexachlorocyclohexane (HCH) and lindane (the purified insecticidal isomer γ-HCH) are prohibited in most countries, residual concentrations still constitute an immense environmental burden. Many studies describe the mineralization of γ-HCH by bacterial strains under aerobic conditions. However, the metabolic fate of the other HCH isomers is not well known. In this study, we investigated the transformation of α-, β-, γ-, δ-, ε-HCH, and a heptachlorocyclohexane isomer in the presence of varying ratios of the two enzymes that initiate γ-HCH degradation, a dehydrochlorinase (LinA) and a haloalkane dehalogenase (LinB). Each substrate yielded a unique metabolic profile that was strongly dependent on the enzyme ratio. Comparison of these results to those of in vivo experiments with different bacterial isolates showed that HCH transformation in the tested strains was highly optimized towards productive metabolism of γ-HCH and that under these conditions other HCH-isomers were metabolized to mixtures of dehydrochlorinated and hydroxylated side-products. In view of these results, bioremediation efforts need very careful planning and toxicities of accumulating metabolites need to be evaluated. © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

Biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of Cucurbita moschata that accumulate recombinant bacterial LinA.

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Nanasato, Yoshihiko; Namiki, Sayuri; Oshima, Masao; Moriuchi, Ryota; Konagaya, Ken-Ichi; Seike, Nobuyasu; Otani, Takashi; Nagata, Yuji; Tsuda, Masataka; Tabei, Yutaka

2016-09-01

γ-HCH was successfully degraded using LinA-expressed transgenic hairy root cultures of Cucurbita moschata . Fusing an endoplasmic reticulum-targeting signal peptide to LinA was essential for stable accumulation in the hairy roots. The pesticide γ-hexachlorocyclohexane (γ-HCH) is a persistent organic pollutant (POP) that raises public health and environmental pollution concerns worldwide. Although several isolates of γ-HCH-degrading bacteria are available, inoculating them directly into γ-HCH-contaminated soil is ineffective because of the bacterial survival rate. Cucurbita species incorporate significant amounts of POPs from soils compared with other plant species. Here, we describe a novel bioremediation strategy that combines the bacterial degradation of γ-HCH and the efficient uptake of γ-HCH by Cucurbita species. We produced transgenic hairy root cultures of Cucurbita moschata that expressed recombinant bacterial linA, isolated from the bacterium Sphingobium japonicum UT26. The LinA protein was accumulated stably in the hairy root cultures by fusing an endoplasmic reticulum (ER)-targeting signal peptide to LinA. Then, we demonstrated that the cultures degraded more than 90 % of γ-HCH (1 ppm) overnight and produced the γ-HCH metabolite 1,2,4-trichlorobenzene, indicating that LinA degraded γ-HCH. These results indicate that the gene linA has high potential for phytoremediation of environmental γ-HCH.

LINS Curve in Romanian Economy

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Emilian Dobrescu

2016-02-01

Full Text Available The paper presents theoretical considerations and empirical evidence to test the validity of the Laffer in Narrower Sense (LINS curve as a parabola with a maximum. Attention is focused on the so-called legal-effective tax gap (letg. The econometric application is based on statistical data (1990-2013 for Romania as an emerging European economy. Three cointegrating regressions (fully modified least squares, canonical cointegrating regression and dynamic least squares and three algorithms, which are based on instrumental variables (two-stage least squares, generalized method of moments, and limited information maximum likelihood, are involved.

[Professor LIN Guohua's experience of gold implantation at acupoint for rheumatoid arthritis].

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Li, Jingjing; Pei, Wenya

2015-12-01

Based on the pathogenesis and symptom of rheumatoid arthritis (RA), professor LIN Guohua's unique opinion and method for RA in clinical treatment are summarized and analyzed. In the opinion of Professor Lin, RA is considered as "Jinbi" and "Gubi" in TCM, which is caused by deficient root with superficial excess. Based on the symptoms of RA, attention should be focused on lung-kidney diagnosis and treatment, and gold and catgut implantation at acupoint can be mutually combined, which is aimed to provide a special and effective method for clinical treatment of RA.

Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa

International Nuclear Information System (INIS)

Sokolova, O.S.; Shaburova, O.V.; Pechnikova, E.V.; Shaytan, A.K.; Krylov, S.V.; Kiselev, N.A.; Krylov, V.N.

2014-01-01

A unique feature of the Pseudomonas aeruginosa giant phage phiKZ is its way of genome packaging onto a spool-like protein structure, the inner body. Until recently, no similar structures have been detected in other phages. We have studied DNA packaging in P. aeruginosa phages EL and Lin68 using cryo-electron microscopy and revealed the presence of inner bodies. The shape and positioning of the inner body and the density of the DNA packaging in EL are different from those found in phiKZ and Lin68. This internal organization explains how the shorter EL genome is packed into a large EL capsid, which has the same external dimensions as the capsids of phiKZ and Lin68. The similarity in the structural organization in EL and other phiKZ-like phages indicates that EL is phylogenetically related to other phiKZ-like phages, and, despite the lack of detectable DNA homology, EL, phiKZ, and Lin68 descend from a common ancestor. - Highlights: • We performed a comparative structural study of giant P. aeruginosa phages: EL, Lin68 and phiKZ. • We revealed that the inner body is a common feature in giant phages. • The phage genome size correlates with the overall dimensions of the inner body

Genome packaging in EL and Lin68, two giant phiKZ-like bacteriophages of P. aeruginosa

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Sokolova, O.S., E-mail: sokolova@mail.bio.msu.ru [M.V. Lomonosov Moscow State University, Moscow (Russian Federation); A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Shaburova, O.V. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation); Pechnikova, E.V. [A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Shaytan, A.K. [M.V. Lomonosov Moscow State University, Moscow (Russian Federation); Krylov, S.V. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation); Kiselev, N.A. [A.V. Shoubnikov Institute of Crystallography RAS, Moscow (Russian Federation); Krylov, V.N. [I.I. Mechnikov Research Institute of Vaccines and Sera, RAMS, Moscow (Russian Federation)

2014-11-15

A unique feature of the Pseudomonas aeruginosa giant phage phiKZ is its way of genome packaging onto a spool-like protein structure, the inner body. Until recently, no similar structures have been detected in other phages. We have studied DNA packaging in P. aeruginosa phages EL and Lin68 using cryo-electron microscopy and revealed the presence of inner bodies. The shape and positioning of the inner body and the density of the DNA packaging in EL are different from those found in phiKZ and Lin68. This internal organization explains how the shorter EL genome is packed into a large EL capsid, which has the same external dimensions as the capsids of phiKZ and Lin68. The similarity in the structural organization in EL and other phiKZ-like phages indicates that EL is phylogenetically related to other phiKZ-like phages, and, despite the lack of detectable DNA homology, EL, phiKZ, and Lin68 descend from a common ancestor. - Highlights: • We performed a comparative structural study of giant P. aeruginosa phages: EL, Lin68 and phiKZ. • We revealed that the inner body is a common feature in giant phages. • The phage genome size correlates with the overall dimensions of the inner body.

Identification and Characterization of Genes That Interact with Lin-12 in Caenorhabditis Elegans

OpenAIRE

Tax, F. E.; Thomas, J. H.; Ferguson, E. L.; Horvitz, H. R.

1997-01-01

We identified and characterized 14 extragenic mutations that suppressed the dominant egg-laying defect of certain lin-12 gain-of-function mutations. These suppressors defined seven genes: sup-17, lag-2, sel-4, sel-5, sel-6, sel-7 and sel-8. Mutations in six of the genes are recessive suppressors, whereas the two mutations that define the seventh gene, lag-2, are semi-dominant suppressors. These suppressor mutations were able to suppress other lin-12 gain-of-function mutations. The suppressor ...

Variational principles of fluid mechanics and electromagnetism: imposition and neglect of the Lin constraint

International Nuclear Information System (INIS)

Allen, R.R. Jr.

1987-01-01

The Lin constraint has been utilized by a number of authors who have sought to develop Eulerian variational principles in both fluid mechanics and electromagnetics (or plasmadynamics). This dissertation first reviews the work of earlier authors concerning the development of variational principles in both the Eulerian and Lagrangian nomenclatures. In the process, it is shown whether or not the Euler-Lagrange equations that result from the variational principles are equivalent to the generally accepted equations of motion. In particular, it is shown in the case of several Eulerian variational principles that imposition of the Lin constraint results in Euler-Lagrange equations equivalent to the generally accepted equations of motion, whereas neglect of the Lin constraint results in restrictive Euler-Lagrange equations. In an effort to improve the physical motivation behind introduction of the Lin constraint, a new variational constraint is developed based on teh concept of surface forces within a fluid. Additionally, it is shown that a quantity often referred to as the canonical momentum of a charged fluid is not always a constant of the motion of the fluid; and it is demonstrated that there does not exist an unconstrained Eulerian variational principle giving rise to the generally accepted equations of motion for both a perfect fluid and a cold, electromagnetic fluid

Lin28a uses distinct mechanisms of binding to RNA and affects miRNA levels positively and negatively.

Science.gov (United States)

Nowak, Jakub Stanislaw; Hobor, Fruzsina; Downie Ruiz Velasco, Angela; Choudhury, Nila Roy; Heikel, Gregory; Kerr, Alastair; Ramos, Andres; Michlewski, Gracjan

2017-03-01

Lin28a inhibits the biogenesis of let-7 miRNAs by triggering the polyuridylation and degradation of their precursors by terminal uridylyltransferases TUT4/7 and 3'-5' exoribonuclease Dis3l2, respectively. Previously, we showed that Lin28a also controls the production of neuro-specific miRNA-9 via a polyuridylation-independent mechanism. Here we reveal that the sequences and structural characteristics of pre-let-7 and pre-miRNA-9 are eliciting two distinct modes of binding to Lin28a. We present evidence that Dis3l2 controls miRNA-9 production. Finally, we show that the constitutive expression of untagged Lin28a during neuronal differentiation in vitro positively and negatively affects numerous other miRNAs. Our findings shed light on the role of Lin28a in differentiating cells and on the ways in which one RNA-binding protein can perform multiple roles in the regulation of RNA processing. © 2017 Nowak et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

Crystal structures of MW1337R and lin2004: Representatives of a novel protein family that adopt a four-helical bundle fold

Energy Technology Data Exchange (ETDEWEB)

Kozbial, Piotr; Xu, Qingping; Chiu, Hsiu-Ju; McMullan, Daniel; Krishna, S. Sri; Miller, Mitchell D.; Abdubek, Polat; Acosta, Claire; Astakhova, Tamara; Axelrod, Herbert L.; Carlton, Dennis; Clayton, Thomas; Deller, Marc; Duan, Lian; Elias, Ylva; Elsliger, Marc-André; Feuerhelm, Julie; Grzechnik, Slawomir K.; Hale, Joanna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Koesema, Eric; Kumar, Abhinav; Marciano, David; Morse, Andrew T.; Murphy, Kevin D.; Nigoghossian, Edward; Okach, Linda; Oommachen, Silvya; Reyes, Ron; Rife, Christopher L.; Spraggon, Glen; Trout, Christina V.; ban den Bedem, Henry; Weekes, Dana; White, Aprilfawn; Wolf, Guenter; Zubieta, Chloe; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A. (Scripps); (SSRL); (JCSG); (UCSD); (Burnham)

2009-08-28

of MW1337R and lin2004, which were determined using the semiautomated, high-throughput pipeline of the Joint Center for Structural Genomics (JCSG), part of the National Institute of General Medical Sciences Protein Structure Initiative.

Combustion of Pure, Hydrolyzed and Methyl Ester Formed of Jatropha Curcas Lin oil

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Muhaji Muhaji

2015-10-01

Full Text Available The density and viscosity of vegetable oil are higher than that of diesel oil. Thus its direct combustion in the diesel engine results many problems. This research was conducted to investigate the flame characteristics of combustion of jatropha curcas lin in pure, hydrolyzed and methyl ester form. The results indicated that the combustion of pure jatropha curcas lin occurs in three stages, hydrolyzed in two stages    and methyl ester in one stage. For pure jatropha curcas lin, in the first stage, unsaturated fatty acid burned for  0.265 s.  It is followed by saturated fatty acid, burned for 0.389 s in the second stage. And, in the last stage is the burned of glycerol for 0.560 s. Meanwhile for hydrolyzed one, in the first stage, unsaturated fatty acid burned for 0.736 s, followed by saturated fatty acid, burned  for 0.326 s in the second stage. And the last, for methyl ester is the burned for 0.712 s. The highest burning rate was for methyl ester which was 0.003931cc/s. The energy releasing rate of methyl ester, which was for 13,628.67 kcal/(kg.s resembled that of diesel oil the most, while the lowest rate was for pure jatropha curcas lin which was 8,200.94 kcal/(kg.s. In addition, massive explosion occurred in the fuel containing unsaturated fatty acid and glycerol

Analysis of a lin-42/period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing

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Theresa L. B. Edelman

2016-12-01

Full Text Available The Caenorhabditis elegans heterochronic gene pathway regulates the relative timing of events during postembryonic development. lin-42, the worm homolog of the circadian clock gene, period, is a critical element of this pathway. lin-42 function has been defined by a set of hypomorphic alleles that cause precocious phenotypes, in which later developmental events, such as the terminal differentiation of hypodermal cells, occur too early. A subset of alleles also reveals a significant role for lin-42 in molting; larval stages are lengthened and ecdysis often fails in these mutant animals. lin-42 is a complex locus, encoding overlapping and nonoverlapping isoforms. Although existing alleles that affect subsets of isoforms have illuminated important and distinct roles for this gene in developmental timing, molting, and the decision to enter the alternative dauer state, it is essential to have a null allele to understand all of the roles of lin-42 and its individual isoforms. To remedy this problem and discover the null phenotype, we engineered an allele that deletes the entire lin-42 protein-coding region. lin-42 null mutants are homozygously viable, but have more severe phenotypes than observed in previously characterized hypomorphic alleles. We also provide additional evidence for this conclusion by using the null allele as a base for reintroducing different isoforms, showing that each isoform can provide heterochronic and molting pathway activities. Transcript levels of the nonoverlapping isoforms appear to be under coordinate temporal regulation, despite being driven by independent promoters. The lin-42 null allele will continue to be an important tool for dissecting the functions of lin-42 in molting and developmental timing.

LIN28 expression in malignant germ cell tumors down-regulates let-7 and increases oncogene levels

Science.gov (United States)

Murray, Matthew J.; Saini, Harpreet K.; Siegler, Charlotte A.; Hanning, Jennifer E.; Barker, Emily M.; van Dongen, Stijn; Ward, Dawn M.; Raby, Katie L.; Groves, Ian J.; Scarpini, Cinzia G.; Pett, Mark R.; Thornton, Claire M.; Enright, Anton J.; Nicholson, James C.; Coleman, Nicholas

2013-01-01

Despite their clinico-pathologic heterogeneity, malignant germ-cell-tumors (GCTs) share molecular abnormalities that are likely to be functionally important. In this study, we investigated the potential significance of down-regulation of the let-7 family of tumor-suppressor microRNAs in malignant-GCTs. Microarray results from pediatric and adult samples (n=45) showed that LIN28, the negative-regulator of let-7 biogenesis, was abundant in malignant-GCTs, regardless of patient age, tumor site or histologic subtype. Indeed, a strong negative-correlation existed between LIN28 and let-7 levels in specimens with matched datasets. Low let-7 levels were biologically significant, since the sequence complementary to the 2-7nt common let-7 seed ‘GAGGUA’ was enriched in the 3′untranslated regions of mRNAs up-regulated in pediatric and adult malignant-GCTs, compared with normal gonads (a mixture of germ cells and somatic cells). We identified 27 mRNA targets of let-7 that were up-regulated in malignant-GCT cells, confirming significant negative-correlations with let-7 levels. Among 16 mRNAs examined in a largely independent set of specimens by qRT-PCR, we defined negative-associations with let-7e levels for six oncogenes, including MYCN, AURKB, CCNF, RRM2, MKI67 and C12orf5 (when including normal control tissues). Importantly, LIN28 depletion in malignant-GCT cells restored let-7 levels and repressed all of these oncogenic let-7 mRNA targets, with LIN28 levels correlating with cell proliferation and MYCN levels. Conversely, ectopic expression of let-7e was sufficient to reduce proliferation and down-regulate MYCN, AURKB and LIN28, the latter via a double-negative feedback loop. We concluded that the LIN28/let-7 pathway has a critical pathobiological role in malignant-GCTs and therefore offers a promising target for therapeutic intervention. PMID:23774216

A role for Lin28 in primordial germ cell development and germ cell malignancy

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West, Jason A.; Viswanathan, Srinivas R.; Yabuuchi, Akiko; Cunniff, Kerianne; Takeuchi, Ayumu; Park, In-Hyun; Sero, Julia E.; Zhu, Hao; Perez-Atayde, Antonio; Frazier, A. Lindsay; Surani, M. Azim; Daley, George Q.

2009-01-01

The rarity and inaccessibility of the earliest primordial germ cells (PGCs) in the mouse embryo thwarts efforts to investigate molecular mechanisms of germ cell specification. Stella marks the minute founder population of the germ lineage1,2. Here we differentiate mouse embryonic stem cells (ESCs) carrying a Stella transgenic reporter into putative PGCs in vitro. The Stella+ cells possess a transcriptional profile similar to embryo-derived PGCs, and like their counterparts in vivo, lose imprints in a time-dependent manner. Using inhibitory RNAs to screen candidate genes for effects on the development of Stella+ cells in vitro, we discovered that Lin28, a negative regulator of let-7 microRNA processing3-6, is essential for proper PGC development. We further show that Blimp1, a let-7 target and a master regulator of PGC specification7-9, can rescue the effect of Lin28-deficiency during PGC development, thereby establishing a mechanism of action for Lin28 during PGC specification. Over-expression of Lin28 promotes formation of Stella+ cells in vitro and PGCs in chimeric embryos, and is associated with human germ cell tumours. The differentiation of putative PGCs from ESCs in vitro recapitulates the early stages of gamete development in vivo, and provides an accessible system for discovering novel genes involved in germ cell development and malignancy. PMID:19578360

LIN7A depletion disrupts cerebral cortex development, contributing to intellectual disability in 12q21-deletion syndrome.

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Ayumi Matsumoto

Full Text Available Interstitial deletion of 12q21 has been reported in four cases, which share several common clinical features, including intellectual disability (ID, low-set ears, and minor cardiac abnormalities. Comparative genomic hybridization (CGH analysis using the Agilent Human Genome CGH 180K array was performed with the genomic DNA from a two-year-old Japanese boy with these symptoms, as well as hypoplasia of the corpus callosum. Consequently, a 14 Mb deletion at 12q21.2-q21.33 (nt. 77 203 574-91 264 613 bp, which includes 72 genes, was detected. Of these, we focused on LIN7A, which encodes a scaffold protein that is important for synaptic function, as a possible responsible gene for ID, and we analyzed its role in cerebral cortex development. Western blotting analyses revealed that Lin-7A is expressed on embryonic day (E 13.5, and gradually increases in the mouse brain during the embryonic stage. Biochemical fractionation resulted in the enrichment of Lin-7A in the presynaptic fraction. Suppression of Lin-7A expression by RNAi, using in utero electroporation on E14.5, delayed neuronal migration on postnatal day (P 2, and Lin-7A-deficient neurons remained in the lower zone of the cortical plate and the intermediate zone. In addition, when Lin-7A was silenced in cortical neurons in one hemisphere, axonal growth in the contralateral hemisphere was delayed; development of these neurons was disrupted such that one half did not extend into the contralateral hemisphere after leaving the corpus callosum. Taken together, LIN7A is a candidate gene responsible for 12q21-deletion syndrome, and abnormal neuronal migration and interhemispheric axon development may contribute to ID and corpus callosum hypoplasia, respectively.

Functional comparison of the nematode Hox gene lin-39 in C. elegans and P. pacificus reveals evolutionary conservation of protein function despite divergence of primary sequences.

Science.gov (United States)

Grandien, K; Sommer, R J

2001-08-15

Hox transcription factors have been implicated in playing a central role in the evolution of animal morphology. Many studies indicate the evolutionary importance of regulatory changes in Hox genes, but little is known about the role of functional changes in Hox proteins. In the nematodes Pristionchus pacificus and Caenorhabditis elegans, developmental processes can be compared at the cellular, genetic, and molecular levels and differences in gene function can be identified. The Hox gene lin-39 is involved in the regulation of nematode vulva development. Comparison of known lin-39 mutations in P. pacificus and C. elegans revealed both conservation and changes of gene function. Here, we study evolutionary changes of lin-39 function using hybrid transgenes and site-directed mutagenesis in an in vivo assay using C. elegans lin-39 mutants. Our data show that despite the functional differences of LIN-39 between the two species, Ppa-LIN-39, when driven by Cel-lin-39 regulatory elements, can functionally replace Cel-lin-39. Furthermore, we show that the MAPK docking and phosphorylation motifs unique for Cel-LIN-39 are dispensable for Cel-lin-39 function. Therefore, the evolution of lin-39 function is driven by changes in regulatory elements rather than changes in the protein itself.

Lin28a uses distinct mechanisms of binding to RNA and affects miRNA levels positively and negatively

OpenAIRE

Nowak, Jakub Stanislaw; Hobor, Fruzsina; Downie Ruiz Velasco, Angela; Choudhury, Nila Roy; Heikel, Gregory; Kerr, Alastair; Ramos, Andres; Michlewski, Gracjan

2017-01-01

Lin28a inhibits the biogenesis of let-7 miRNAs by triggering the polyuridylation and degradation of their precursors by terminal uridylyltransferases TUT4/7 and 3’-5’ exoribonuclease Dis3l2, respectively. Previously, we showed that Lin28a also controls the production of neuro-specific miRNA-9 via a polyuridylation-independent mechanism. Here we reveal that the sequences and structural characteristics of pre-let-7 and pre-miRNA-9 are eliciting two distinct modes of binding to Lin28a. We presen...

Response to Niklasson's comment on Lin, et al. (2012) : "the relation between postural movement and bilateral motor integration".

Science.gov (United States)

Lin, Chin-Kai; Kuo, Bor-Chen; Wu, Huey-Min

2014-10-01

In the study of Lin, Wu, Lin, Wu, Wu, Kuo, and Yeung (2012 ), the relationship between the validity of postural movement and bilateral motor integration in terms of sensory integration theory was examined. Postural movement is the ability to use the antigravity postures required for stabilization of the neck, trunk and upper extremities via muscle co-contractions in the neck and upper extremities, and balance. Niklasson's (2013 ) comment argued that postural movement should include primitive reflexes in terms of the general abilities approach. Niklasson (2013 ) focused on the efficacy of the treatment rather than the theoretical frameworks implied in the therapeutic activities. For that purpose Lin, et al. (2012 ) used sensory integration as the theoretical foundation, and the relationship between postural movement and bilateral motor integration was assessed via empirical data. The result of Lin, et al. (2012 ) was offered as a theoretical reference for therapeutic activities.

The let-7/Lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury.

Science.gov (United States)

McDaniel, Kelly; Huang, Li; Sato, Keisaku; Wu, Nan; Annable, Tami; Zhou, Tianhao; Ramos-Lorenzo, Sugeily; Wan, Ying; Huang, Qiaobing; Francis, Heather; Glaser, Shannon; Tsukamoto, Hidekazu; Alpini, Gianfranco; Meng, Fanyin

2017-07-07

The let-7/Lin28 axis is associated with the regulation of key cellular regulatory genes known as microRNAs in various human disorders and cancer development. This study evaluated the role of the let-7/Lin28 axis in regulating a mesenchymal phenotype of hepatic stellate cells in alcoholic liver injury. We identified that ethanol feeding significantly down-regulated several members of the let-7 family in mouse liver, including let-7a and let-7b. Similarly, the treatment of human hepatic stellate cells (HSCs) with lipopolysaccharide (LPS) and transforming growth factor-β (TGF-β) significantly decreased the expressions of let-7a and let-7b. Conversely, overexpression of let-7a and let-7b suppressed the myofibroblastic activation of cultured human HSCs induced by LPS and TGF-β, as evidenced by repressed ACTA2 (α-actin 2), COL1A1 (collagen 1A1), TIMP1 (TIMP metallopeptidase inhibitor 1), and FN1 (fibronectin 1); this supports the notion that HSC activation is controlled by let-7. A combination of bioinformatics, dual-luciferase reporter assay, and Western blot analysis revealed that Lin28B and high-mobility group AT-hook (HMGA2) were the direct targets of let-7a and let-7b. Furthermore, Lin28B deficiency increased the expression of let-7a/let-7b as well as reduced HSC activation and liver fibrosis in mice with alcoholic liver injury. This feedback regulation of let-7 by Lin28B is verified in hepatic stellate cells isolated by laser capture microdissection from the model. The identification of the let-7/Lin28 axis as an important regulator of HSC activation as well as its upstream modulators and down-stream targets will provide insights into the involvement of altered microRNA expression in contributing to the pathogenesis of alcoholic liver fibrosis and novel therapeutic approaches for human alcoholic liver diseases. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Comparison of arterial blood gas with continuous intra-arterial and transcutaneous PO2 sensors in adult critically ill patients.

Science.gov (United States)

Green, G E; Hassell, K T; Mahutte, C K

1987-05-01

We compared the partial pressure of oxygen directly via a continuous intra-arterial probe (PiaO2) and indirectly using a transcutaneous device (PtcO2) with simultaneously obtained arterial blood PaO2. The PiaO2 values were measured using a bipolar oxygen sensor placed through an 18-ga arterial catheter. The PtcO2 values were measured using a transcutaneous O2-CO2 sensor placed on the abdomen. Seven critically ill, hemodynamically stable, ventilator-dependent adult patients were studied. Measurements were obtained at varying concentrations (0.25 to 1.0) of inspired oxygen after a 10-min stabilization. A total of 78 simultaneous values were obtained; by linear regression: PiaO2 = 0.91 PaO2 + 1.39 (r = .98, standard errors of the estimate [SEE] = 18.6); PtcO2 = 0.39 PaO2 + 36.2 (r = .89, SEE = 14.1). To assess these instruments as trend monitors, we compared the changes in simultaneous PaO2, PiaO2, and PtcO2 values; by linear regression: delta PiaO2 = 0.90 delta PaO2 + 3.88 (r = .96, SEE = 27.7); delta PtcO2 = 0.43 delta PaO2 + 5.6 (r = .94, SEE = 15.2). We conclude that, although these instruments correlate highly with the PaO2, the SEE was substantial and therefore may limit their clinical reliability in adults. Any acute or clinically significant change in PiaO2 or PtcO2 should be confirmed with a blood gas PaO2.

Opposing Post-transcriptional Control of InR by FMRP and LIN-28 Adjusts Stem Cell-Based Tissue Growth

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Arthur Luhur

2017-12-01

Full Text Available Summary: Although the intrinsic mechanisms that control whether stem cells divide symmetrically or asymmetrically underlie tissue growth and homeostasis, they remain poorly defined. We report that the RNA-binding protein fragile X mental retardation protein (FMRP limits the symmetric division, and resulting expansion, of the stem cell population during adaptive intestinal growth in Drosophila. The elevated insulin sensitivity that FMRP-deficient progenitor cells display contributes to their accelerated expansion, which is suppressed by the depletion of insulin-signaling components. This FMRP activity is mediated solely via a second conserved RNA-binding protein, LIN-28, known to boost insulin signaling in stem cells. Via LIN-28, FMRP controls progenitor cell behavior by post-transcriptionally repressing the level of insulin receptor (InR. This study identifies the stem cell-based mechanism by which FMRP controls tissue adaptation, and it raises the possibility that defective adaptive growth underlies the accelerated growth, gastrointestinal, and other symptoms that affect fragile X syndrome patients. : Luhur et al. report that FMRP acts via LIN-28 in progenitor cells to dampen the adaptive expansion of intestinal tissue in the fruit fly, raising the possibility that defective LIN28-mediated adaptive growth underlies some of the symptoms that affect fragile X syndrome patients. Keywords: FMRP, fmr1, LIN-28, insulin receptor, IIS, adaptive growth, tissue resizing, intestinal stem cell, insulin sensitivity

Fragmented Encounters, Social Slippages: Lin Huiyin's "In Ninety-Nine Degree Heat"

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Carles Prado-Fonts

2010-01-01

Full Text Available The article reads Lin Huiyin’s short story “In Ninety-Nine Degree Heat” (1934 in relation to the context of 1930s China, as an innovative literary work which combines elements from both the Chinese and the Western traditions, and as a text which informs readers not only of the problematic of class and gender issues in 1930s Chinese society but also of the context of the liuxuesheng who returns to China –like Lin Huiyin herself. Focusing on questions like otherness, representation, and encounters, the essay analyzes how the episodic narrative structure of Lin’s short story echoes social and representational discourses in post-May Fourth China, at the same time that it explores issues such as social inequality, otherness and alienation, which were crucial to the liuxuesheng, and which reflect Lin’s own experience as a returned and alienated liuxuesheng at the time.

Overexpression of Lin28b in Neural Stem Cells is Insufficient for Brain Tumor Formation, but Induces Pathological Lobulation of the Developing Cerebellum.

Science.gov (United States)

Wefers, Annika K; Lindner, Sven; Schulte, Johannes H; Schüller, Ulrich

2017-02-01

LIN28B is a homologue of the RNA-binding protein LIN28A and regulates gene expression during development and carcinogenesis. It is strongly upregulated in a variety of brain tumors, such as medulloblastoma, embryonal tumor with multilayered rosettes (ETMR), atypical teratoid/rhabdoid tumor (AT/RT), or glioblastoma, but the effect of an in vivo overexpression of LIN28B on the developing central nervous system is unknown. We generated transgenic mice that either overexpressed Lin28b in Math1-positive cerebellar granule neuron precursors or in a broad range of Nestin-positive neural precursors. Sections of the cerebellar vermis from adult Math1-Cre::lsl-Lin28b mice had an additional subfissure in lobule IV. Vermes from p0 and p7 Nestin-Cre::lsl-Lin28b mice appeared normal, but we found a pronounced vermal hypersublobulation at p15 and p21 in these mice. Also, the external granule cell layer (EGL) was thicker at p15 than in controls, contained more proliferating cells, and persisted up to p21. Consistently, some Pax6- and NeuN-positive cells were present in the EGL of Nestin-Cre::lsl-Lin28b mice even at p21, and we detected more NeuN-positive granule neuron precursors in the molecular layer (ML) as compared to control. Finally, we found some residual Pax2-positive precursors of inhibitory interneurons in the ML of Nestin-Cre::lsl-Lin28b mice at p21, which have already disappeared in controls. We conclude that while overexpression of LIN28B in Nestin-positive cells does not lead to tumor formation, it results in a protracted development of granule cells and inhibitory interneurons and leads to a hypersublobulation of the cerebellar vermis.

93 une urbanisation linéaire, dynamique demographique et ...

African Journals Online (AJOL)

Mohand

d'Alger, dans l'ensemble Sahel-Mitidja sur un linéaire côtier de 2 km. Rattaché administrativement à la daira de ... connait une dynamique démographique remar- quable, dans sa périphérie orientale - composé de l'ensemble Sahel-. Mitidja ..... «Etude agro-pédologique de la plaine de la Mitidja». Florin B.; Semoud N. 2010.

Dynamiques complexes et morphogenèse Introduction aux sciences non linéaires

CERN Document Server

Misbah, Chaouqi

2011-01-01

Les sciences non linéaires ont pour objet l’ensemble des phénomènes dont l’analyse résiste au principe de superposition. Elles concernent en grande partie les systèmes dits « complexes » dont l’interaction et l’interdépendance entre les parties empêchent de prédire précisément l’évolution du système. Pour expliquer ces phénomènes, deux approches complémentaires ont été proposées : la théorie des bifurcations et la théorie des catastrophes. Mais la pleine compréhension et la modélisation de la non-linéarité restent chacune un défi pour les scientifiques du XXIe siècle. C’est dans la perspective d’accompagner tous ceux qui voudront le relever que ce livre a été conçu. Son objectif est d’exposer au lecteur le langage et le formalisme nécessaires à l’étude de la non-linéarité. Partant d’exemples simples, pour ensuite atteindre un niveau d’abstraction visant l’universalité, l’auteur explore les divers scénarios possibles de bifurcations et les catastr...

[Analysis of acid rain characteristics of Lin'an Regional Background Station using long-term observation data].

Science.gov (United States)

Li, Zheng-Quan; Ma, Hao; Mao, Yu-Ding; Feng, Tao

2014-02-01

Using long-term observation data of acid rain at Lin'an Regional Background Station (Lin'an RBS), this paper studied the interannual and monthly variations of acid rain, the reasons for the variations, and the relationships between acid rain and meteorological factors. The results showed that interannual variation of acid rain at Lin'an RBS had a general increasing trend in which there were two obvious intensifying processes and two distinct weakening processes, during the period ranging from 1985 to 2012. In last two decades, the monthly variation of acid rain at Lin'an RBS indicated that rain acidity and frequency of severe acid rain were increasing but the frequency of weak acid rain was decreasing when moving towards bilateral side months of July. Acid rain occurrence was affected by rainfall intensity, wind speed and wind direction. High frequency of severe acid rain and low frequency of weak acid rain were on days with drizzle, but high frequency of weak acid rain and low frequency of severe acid rain occurred on rainstorm days. With wind speed upgrading, the frequency of acid rain and the proportion of severe acid rain were declining, the pH value of precipitation was reducing too. Another character is that daily dominant wind direction of weak acid rain majorly converged in S-W section ,however that of severe acid rain was more likely distributed in N-E section. The monthly variation of acid rain at Lin'an RBS was mainly attributed to precipitation variation, the increasing and decreasing of monthly incoming wind from SSE-WSW and NWN-ENE sections of wind direction. The interannual variation of acid rain could be due to the effects of energy consumption raising and significant green policies conducted in Zhejiang, Jiangsu and Shanghai.

Primordial dwarfism gene maintains Lin28 expression to safeguard embryonic stem cells from premature differentiation.

Science.gov (United States)

Dai, Qian; Luan, Guangxin; Deng, Li; Lei, Tingjun; Kang, Han; Song, Xu; Zhang, Yujun; Xiao, Zhi-Xiong; Li, Qintong

2014-05-08

Primordial dwarfism (PD) is characterized by global growth failure, both during embryogenesis and postnatally. Loss-of-function germline mutations in La ribonucleoprotein domain family, member 7 (LAPR7) have recently been linked to PD. Paradoxically, LARP7 deficiency was previously assumed to be associated with increased cell growth and proliferation via activation of positive transcription elongation factor b (P-TEFb). Here, we show that Larp7 deficiency likely does not significantly increase P-TEFb activity. We further discover that Larp7 knockdown does not affect pluripotency but instead primes embryonic stem cells (ESCs) for differentiation via downregulation of Lin28, a positive regulator of organismal growth. Mechanistically, we show that Larp7 interacts with a poly(A) polymerase Star-PAP to maintain Lin28 mRNA stability. We propose that proper regulation of Lin28 and PTEFb is essential for embryonic cells to achieve a sufficient number of cell divisions prior to differentiation and ultimately to maintain proper organismal size. Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

New explicit and exact solutions of the Benney–Kawahara–Lin equation

International Nuclear Information System (INIS)

Yuan-Xi, Xie

2009-01-01

In this paper, we present a combination method of constructing the explicit and exact solutions of nonlinear partial differential equations. And as an illustrative example, we apply the method to the Benney–Kawahara–Lin equation and derive its many explicit and exact solutions which are all new solutions. (general)

Välinäituse stendid = Outdoor exhibition stands / Gert Sarv, Galina Burnakova

Index Scriptorium Estoniae

Sarv, Gert, 1971-

2013-01-01

Eesti Sisearhitektide Liidu 2012. aasta parima eseme preemia pälvinud välinäituse stendidest Vanasadama Admiraliteedi basseini kaldapealsel. Autorid: Gert Sarv, Galina Burnakova, Loreida Hein. Sisearhitekti ja ESLi aastapreemiate žürii liikme Tea Tammelaane arvamus

DPL-1 DP, LIN-35 Rb and EFL-1 E2F act with the MCD-1 zinc-finger protein to promote programmed cell death in Caenorhabditis elegans.

Science.gov (United States)

Reddien, Peter W; Andersen, Erik C; Huang, Michael C; Horvitz, H Robert

2007-04-01

The genes egl-1, ced-9, ced-4, and ced-3 play major roles in programmed cell death in Caenorhabditis elegans. To identify genes that have more subtle activities, we sought mutations that confer strong cell-death defects in a genetically sensitized mutant background. Specifically, we screened for mutations that enhance the cell-death defects caused by a partial loss-of-function allele of the ced-3 caspase gene. We identified mutations in two genes not previously known to affect cell death, dpl-1 and mcd-1 (modifier of cell death). dpl-1 encodes the C. elegans homolog of DP, the human E2F-heterodimerization partner. By testing genes known to interact with dpl-1, we identified roles in cell death for four additional genes: efl-1 E2F, lin-35 Rb, lin-37 Mip40, and lin-52 dLin52. mcd-1 encodes a novel protein that contains one zinc finger and that is synthetically required with lin-35 Rb for animal viability. dpl-1 and mcd-1 act with efl-1 E2F and lin-35 Rb to promote programmed cell death and do so by regulating the killing process rather than by affecting the decision between survival and death. We propose that the DPL-1 DP, MCD-1 zinc finger, EFL-1 E2F, LIN-35 Rb, LIN-37 Mip40, and LIN-52 dLin52 proteins act together in transcriptional regulation to promote programmed cell death.

GENII-LIN: a Multipurpose Health Physics Code Built on GENII-1.485

Directory of Open Access Journals (Sweden)

Marco Sumini

2006-10-01

Full Text Available The aim of the GENII-LIN project was to develop an open source multipurpose health physics code running on Linux platform, for calculating radiation dose and risk from radionuclides released to the environment. The general features of the GENII-LIN system include [1] capabilities for calculating radiation dose both for acute and chronic releases, with options for annual dose, committed dose and accumulated dose [2] capabilities for evaluating exposure pathways including direct exposure via water (swimming, boating, fishing, soil (buried and surface sources and air (semi-infinite cloud and finite cloud model, inhalation pathways and ingestion pathways. The release scenarios considered are: - acute release to air, from ground level or elevated sources, or to water; - chronic release to air, from ground level or elevated sources, or to water; - initial contamination of soil or surfaces. Keywords: radiation protection, Linux, health physics, risk analysis.

Using Lin's method to solve Bykov's problems

Science.gov (United States)

Knobloch, Jürgen; Lamb, Jeroen S. W.; Webster, Kevin N.

2014-10-01

We consider nonwandering dynamics near heteroclinic cycles between two hyperbolic equilibria. The constituting heteroclinic connections are assumed to be such that one of them is transverse and isolated. Such heteroclinic cycles are associated with the termination of a branch of homoclinic solutions, and called T-points in this context. We study codimension-two T-points and their unfoldings in Rn. In our consideration we distinguish between cases with real and complex leading eigenvalues of the equilibria. In doing so we establish Lin's method as a unified approach to (re)gain and extend results of Bykov's seminal studies and related works. To a large extent our approach reduces the study to the discussion of intersections of lines and spirals in the plane. Case (RR): Under open conditions on the eigenvalues, there exist open sets in parameter space for which there exist periodic orbits close to the heteroclinic cycle. In addition, there exist two one-parameter families of homoclinic orbits to each of the saddle points p1 and p2.See Theorem 2.1 and Proposition 2.2 for precise statements and Fig. 2 for bifurcation diagrams. Cases (RC) and (CC): At the bifurcation point μ=0 and for each N≥2, there exists an invariant set S0N close to the heteroclinic cycle on which the first return map is topologically conjugated to a full shift on N symbols. For any fixed N≥2, the invariant set SμN persists for |μ| sufficiently small.In addition, there exist infinitely many transversal and non-transversal heteroclinic orbits connecting the saddle points p1 and p2 in a neighbourhood of μ=0, as well as infinitely many one-parameter families of homoclinic orbits to each of the saddle points.For full statements of the results see Theorem 2.3 and Propositions 2.4, 2.5 and Fig. 3 for bifurcation diagrams. The dynamics near T-points has been studied previously by Bykov [6-10], Glendinning and Sparrow [20], Kokubu [27,28] and Labouriau and Rodrigues [30,31,38]. See also the surveys

Topografia ficcional: a construção do espaço nos manuscritos de Osman Lins

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Francisco José Gonçalves Lima Rocha

2013-01-01

Full Text Available In the construction of the narrative space of his final, unfinished novel, A cabeça levada em triunfo, Osman Lins operates both verbal, with notes planning, as iconically, through drawings. Will try to show that the author’s purpose is to clarify the topography of the work, because it establishes and harmonizes the technical functions - ie, like space, says Osman Lins, “frame” the character – and symbolic means, ie. how the space reflects, like all other elements of the work, the questioning of the head-body dichotomy.

On the reduction of generalized polylogarithms to Li_n and Li_2_,_2 and on the evaluation thereof

International Nuclear Information System (INIS)

Frellesvig, Hjalte; Tommasini, Damiano; Wever, Christopher

2016-01-01

We give expressions for all generalized polylogarithms up to weight four in terms of the functions log, Li_n, and Li_2_,_2, valid for arbitrary complex variables. Furthermore we provide algorithms for manipulation and numerical evaluation of Li_n and Li_2_,_2, and add codes in Mathematica and C++ implementing the results. With these results we calculate a number of previously unknown integrals, which we add in appendix C.

Lin28B promotes Müller glial cell de-differentiation and proliferation in the regenerative rat retinas

Science.gov (United States)

Tao, Zui; Zhao, Chen; Jian, Qian; Gillies, Mark; Xu, Haiwei; Yin, Zheng Qin

2016-01-01

Retinal regeneration and repair are severely impeded in higher mammalian animals. Although Müller cells can be activated and show some characteristics of progenitor cells when injured or under pathological conditions, they quickly form gliosis scars. Unfortunately, the basic mechanisms that impede retinal regeneration remain unknown. We studied retinas from Royal College of Surgeon (RCS) rats and found that let-7 family molecules, let-7e and let-7i, were significantly overexpressed in Müller cells of degenerative retinas. It demonstrated that down-regulation of the RNA binding protein Lin28B was one of the key factors leading to the overexpression of let-7e and let-7i. Lin28B ectopic expression in the Müller cells suppressed overexpression of let-7e and let-7i, stimulated and mobilized Müller glia de-differentiation, proliferation, promoted neuronal commitment, and inhibited glial fate acquisition of de-differentiated Müller cells. ERG recordings revealed that the amplitudes of a-wave and b-wave were improved significantly after Lin28B was delivered into the subretinal space of RCS rats. In summary, down-regulation of Lin28B as well as up-regulation of let-7e and let-7i may be the main factors that impede Müller cell de-differentiation and proliferation in the retina of RCS rats. PMID:27384999

The Story of Heathcliff’s Journey Back to Wuthering Heights de Lin Haire-Sargeant (1992, ou le « deux en un » de la ré-écriture The Story of Heathcliff’s Journey Back to Wuthering Heights by Lin Haire-Sargeant (1992, and a “Two in one” Rewrite

Directory of Open Access Journals (Sweden)

Isabelle Roblin

2009-10-01

Full Text Available Lin Haire-Sargeant’s 1992 novel The Story of Heathcliff’s Journey Back to Wuthering Heights is typical of the interest shown by contemporary American writers in Victorian novels. It could indeed be classified doubly as a “retro-Victorian novel” since it partly re-writes not only Emily Brontë’s most famous novel, Wuthering Heights, as the title points out, but also, somewhat less obviously, Charlotte Brontë’s Jane Eyre. We will study in this article how Lin Haire-Sargeant’s attempt at re-writing highlights the usual aims of the retro-Victorian novel: taking advantage of the blanks left by the source novels to propose to the reader a modern critical approach, while at the same time being as faithful as possible to the writing conventions of the 19th century, and suggesting another point of view on a well-known story, through a postmodern mixing of historical and fictive characters.

A role for the malignant brain tumour (MBT domain protein LIN-61 in DNA double-strand break repair by homologous recombination.

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Nicholas M Johnson

Full Text Available Malignant brain tumour (MBT domain proteins are transcriptional repressors that function within Polycomb complexes. Some MBT genes are tumour suppressors, but how they prevent tumourigenesis is unknown. The Caenorhabditis elegans MBT protein LIN-61 is a member of the synMuvB chromatin-remodelling proteins that control vulval development. Here we report a new role for LIN-61: it protects the genome by promoting homologous recombination (HR for the repair of DNA double-strand breaks (DSBs. lin-61 mutants manifest numerous problems associated with defective HR in germ and somatic cells but remain proficient in meiotic recombination. They are hypersensitive to ionizing radiation and interstrand crosslinks but not UV light. Using a novel reporter system that monitors repair of a defined DSB in C. elegans somatic cells, we show that LIN-61 contributes to HR. The involvement of this MBT protein in HR raises the possibility that MBT-deficient tumours may also have defective DSB repair.

Cryptanalysis of Lin et al.'s Efficient Block-Cipher-Based Hash Function

NARCIS (Netherlands)

Liu, Bozhong; Gong, Zheng; Chen, Xiaohong; Qiu, Weidong; Zheng, Dong

2010-01-01

Hash functions are widely used in authentication. In this paper, the security of Lin et al.'s efficient block-cipher-based hash function is reviewed. By using Joux's multicollisions and Kelsey et al.'s expandable message techniques, we find the scheme is vulnerable to collision, preimage and second

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

NARCIS (Netherlands)

Shankaranarayanan, P.; Mendoza-Parra, M.A.; Walia, M.; Wang, L.; Li, N.; Trindade, L.M.; Gronemeyer, H.

2011-01-01

Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts

The gga-let-7 family post-transcriptionally regulates TGFBR1 and LIN28B during the differentiation process in early chick development.

Science.gov (United States)

Lee, Sang In; Jeon, Mi-Hyang; Kim, Jeom Sun; Jeon, Ik-Soo; Byun, Sung June

2015-12-01

Early chick embryogenesis is governed by a complex mechanism involving transcriptional and post-transcriptional regulation, although how post-transcriptional processes influence the balance between pluripotency and differentiation during early chick development have not been previously investigated. Here, we characterized the microRNA (miRNA) signature associated with differentiation in the chick embryo, and found that as expression of the gga-let-7 family increases through early development, expression of their direct targets, TGFBR1 and LIN28B, decreases; indeed, gga-let-7a-5p and gga-let-7b miRNAs directly bind to TGFBR1 and LIN28B transcripts. Our data further indicate that TGFBR1 and LIN28B maintain pluripotency by regulating POUV, NANOG, and CRIPTO. Therefore, gga-let-7 miRNAs act as post-transcriptional regulators of differentiation in blastodermal cells by repressing the expression of the TGFBR1 and LIN28B, which intrinsically controls blastodermal cell differentiation in early chick development. © 2015 Wiley Periodicals, Inc.

Curcumin Inhibits LIN-28A through the Activation of miRNA-98 in the Lung Cancer Cell Line A549.

Science.gov (United States)

Liu, Wei-Lun; Chang, Jia-Ming; Chong, Inn-Wen; Hung, Yi-Li; Chen, Yung-Hsiang; Huang, Wen-Tsung; Kuo, Hsuan-Fu; Hsieh, Chong-Chao; Liu, Po-Len

2017-06-03

Metastasis is common in lung cancer and is associated with poor clinical outcomes and increased mortality. Curcumin is a natural anti-cancer agent that inhibits the metastasis of various cancers by modulating the expression of micro (mi) RNAs such as miR-98, which acts as a tumor suppressor. This study investigated the effect of curcumin on miR-98 expression and in vitro cell line growth and invasiveness in lung cancer. Curcumin treatment enhanced the expression of miR-98 and reduced that of the miR-98 target gene LIN28A as well as matrix metalloproteinase ( MMP ) 2 and MMP9 in vitro and in vivo. MiR-98 overexpression suppressed lung cancer cell migration and invasion by inhibiting LIN28A-induced MMP2 and MMP9 expression. Meanwhile, LIN28A level was downregulated by overexpression of miR-98 mimic. Induction of miR-98 by curcumin treatment suppressed MMP2 and MMP9 by targeting LIN28A. These findings provide insight into the mechanisms by which curcumin suppresses lung cancer cell line growth in vitro and in vivo and invasiveness in vitro.

An Effective Implementation of K-opt Moves for the Lin-Kernighan TSP Heuristic

DEFF Research Database (Denmark)

Helsgaun, Keld

Local search with k-change neighborhoods, k-opt, is the most widely used heuristic method for the traveling salesman problem (TSP). This report presents an effective implementation of k-opt for the Lin- Kernighan TSP heuristic. The effectiveness of the implementation is demonstrated with extensive...

Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry.

Science.gov (United States)

Monincová, Marta; Prokop, Zbynek; Vévodová, Jitka; Nagata, Yuji; Damborsky, Jirí

2007-03-01

1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for the development of a new biocatalyst toward TCP by protein engineering. Microcalorimetry is proposed to be a universal method for the detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering novel biocatalysts using the scaffolds of proteins with promiscuous activities.

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry▿

Science.gov (United States)

Monincová, Marta; Prokop, Zbyněk; Vévodová, Jitka; Nagata, Yuji; Damborský, Jiří

2007-01-01

1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s−1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for the development of a new biocatalyst toward TCP by protein engineering. Microcalorimetry is proposed to be a universal method for the detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering novel biocatalysts using the scaffolds of proteins with promiscuous activities. PMID:17259360

LIN28B polymorphisms are associated with central precocious puberty and early puberty in girls

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Sung Won Park

2012-10-01

Full Text Available &lt;B&gt;Purpose:&lt;/B&gt; Single-nucleotide polymorphism (SNP markers within &lt;i&gt;LIN28B&lt;/i&gt; have been reported to be related to the timing of pubertal growth. However, no study has investigated the frequency of genetic markers in girls with precocious puberty (PP or early puberty (EP. This study aimed to determine the frequency of putative genetic markers in girls with PP or EP. &lt;B&gt;Methods:&lt;/B&gt; Genomic DNAs were obtained from 77 and 109 girls that fulfilled the criteria for PP and EP, respectively. The controls in this study were 144 healthy volunteers between 20 and 30 years of age. The haplotypes were reconstructed using 11 SNPs of &lt;i&gt;LIN28B&lt;/i&gt;, and haplotype association analysis was performed. The haplotype frequencies were compared. Differences in the clinical and laboratory parameters were analyzed according to the haplotype dosage. &lt;B&gt;Results:&lt;/B&gt; Eleven SNPs in &lt;i&gt;LIN28B&lt;/i&gt; were all located in a block that was in linkage disequilibrium. The haplotype could be reconstructed using 2 representative SNPs, rs4946651 and rs369065. The AC haplotype was less frequently observed in the PP group than in the controls (0.069 vs. 0.144, &lt;I&gt;P&lt;/I&gt;=0.010. The trend that girls with non-AC haplotypes tended to have earlier puberty onset (&lt;I&gt;P&lt;/I&gt;=0.037 was illustrated even in the EP+PP patient group by Kaplan-Meier analysis. &lt;B&gt;Conclusion:&lt;/B&gt; The results of the present study showed that non-AC haplotypes of &lt;i&gt;LIN28B&lt;/i&gt;had a significant association with PP in girls.

Loss of the Caenorhabditis elegans pocket protein LIN-35 reveals MuvB's innate function as the repressor of DREAM target genes.

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Paul D Goetsch

2017-11-01

Full Text Available The DREAM (Dp/Retinoblastoma(Rb-like/E2F/MuvB transcriptional repressor complex acts as a gatekeeper of the mammalian cell cycle by establishing and maintaining cellular quiescence. How DREAM's three functional components, the E2F-DP heterodimer, the Rb-like pocket protein, and the MuvB subcomplex, form and function at target gene promoters remains unknown. The current model invokes that the pocket protein links E2F-DP and MuvB and is essential for gene repression. We tested this model by assessing how the conserved yet less redundant DREAM system in Caenorhabditis elegans is affected by absence of the sole C. elegans pocket protein LIN-35. Using a LIN-35 protein null mutant, we analyzed the assembly of E2F-DP and MuvB at promoters that are bound by DREAM and the level of expression of those "DREAM target genes" in embryos. We report that LIN-35 indeed mediates the association of E2F-DP and MuvB, a function that stabilizes DREAM subunit occupancy at target genes. In the absence of LIN-35, the occupancy of E2F-DP and MuvB at most DREAM target genes decreases dramatically and many of those genes become upregulated. The retention of E2F-DP and MuvB at some target gene promoters in lin-35 null embryos allowed us to test their contribution to DREAM target gene repression. Depletion of MuvB, but not E2F-DP, in the sensitized lin-35 null background caused further upregulation of DREAM target genes. We conclude that the pocket protein functions primarily to support MuvB-mediated repression of DREAM targets and that transcriptional repression is the innate function of the evolutionarily conserved MuvB complex. Our findings provide important insights into how mammalian DREAM assembly and disassembly may regulate gene expression and the cell cycle.

Safety and Feasibility of Lin- Cells Administration to ALS Patients: A Novel View on Humoral Factors and miRNA Profiles

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Anna Sobuś

2018-04-01

Full Text Available Therapeutic options for amyotrophic lateral sclerosis (ALS are still limited. Great hopes, however, are placed in growth factors that show neuroprotective abilities (e.g., nerve growth factor (NGF, brain-derived neurotrophic factor (BDNF, and vascular endothelial growth factor (VEGF and in the immune modulating features, in particular, the anti-inflammatory effects. In our study we aimed to investigate whether a bone marrow-derived lineage-negative (Lin- cells population, after autologous application into cerebrospinal fluid (CSF, is able to produce noticeable concentrations of trophic factors and inflammatory-related proteins and thus influence the clinical course of ALS. To our knowledge, the evaluation of Lin- cells transplantation for ALS treatment has not been previously reported. Early hematopoietic Lin- cells were isolated from twelve ALS patients’ bone marrow, and later, the suspension of cells was administered into the subarachnoid space by lumbar puncture. Concentrations of selected proteins in the CSF and plasma were quantified by multiplex fluorescent bead-based immunoassays at different timepoints post-transplantation. We also chose microRNAs (miRNAs related to muscle biology (miRNA-1, miRNA-133a, and miRNA-206 and angiogenesis and inflammation (miRNA-155 and miRNA-378 and tested, for the first time, their expression profiles in the CSF and plasma of ALS patients after Lin- cells transplantation. The injection of bone marrow cells resulted in decreased concentration of selected inflammatory proteins (C3 after Lin- cells injection, particularly in patients who had a better clinical outcome. Moreover, several analyzed miRNAs have changed expression levels in the CSF and plasma of ALS patients subsequent to Lin- cells administration. Interestingly, the expression of miR-206 increased in ALS patients, while miR-378 decreased both in the CSF and plasma one month after the cells’ injection. We propose that autologous lineage

Safety and Feasibility of Lin- Cells Administration to ALS Patients: A Novel View on Humoral Factors and miRNA Profiles.

Science.gov (United States)

Sobuś, Anna; Baumert, Bartłomiej; Litwińska, Zofia; Gołąb-Janowska, Monika; Stępniewski, Jacek; Kotowski, Maciej; Pius-Sadowska, Ewa; Kawa, Miłosz P; Gródecka-Szwajkiewicz, Dorota; Peregud-Pogorzelski, Jarosław; Dulak, Józef; Nowacki, Przemysław; Machaliński, Bogusław

2018-04-27

Therapeutic options for amyotrophic lateral sclerosis (ALS) are still limited. Great hopes, however, are placed in growth factors that show neuroprotective abilities (e.g., nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and vascular endothelial growth factor (VEGF)) and in the immune modulating features, in particular, the anti-inflammatory effects. In our study we aimed to investigate whether a bone marrow-derived lineage-negative (Lin-) cells population, after autologous application into cerebrospinal fluid (CSF), is able to produce noticeable concentrations of trophic factors and inflammatory-related proteins and thus influence the clinical course of ALS. To our knowledge, the evaluation of Lin- cells transplantation for ALS treatment has not been previously reported. Early hematopoietic Lin- cells were isolated from twelve ALS patients’ bone marrow, and later, the suspension of cells was administered into the subarachnoid space by lumbar puncture. Concentrations of selected proteins in the CSF and plasma were quantified by multiplex fluorescent bead-based immunoassays at different timepoints post-transplantation. We also chose microRNAs (miRNAs) related to muscle biology (miRNA-1, miRNA-133a, and miRNA-206) and angiogenesis and inflammation (miRNA-155 and miRNA-378) and tested, for the first time, their expression profiles in the CSF and plasma of ALS patients after Lin- cells transplantation. The injection of bone marrow cells resulted in decreased concentration of selected inflammatory proteins (C3) after Lin- cells injection, particularly in patients who had a better clinical outcome. Moreover, several analyzed miRNAs have changed expression levels in the CSF and plasma of ALS patients subsequent to Lin- cells administration. Interestingly, the expression of miR-206 increased in ALS patients, while miR-378 decreased both in the CSF and plasma one month after the cells’ injection. We propose that autologous lineage-negative early

Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma

Energy Technology Data Exchange (ETDEWEB)

Hayashi, S.; Tanaka, J.; Okada, S.; Isobe, T.; Yamamoto, G.; Yasuhara, R.; Irie, T.; Akiyama, C.; Kohno, Y.; Tachikawa, T.; Mishima, K., E-mail: mishima-k@dent.showa-u.ac.jp

2013-05-01

Cancer stem cells (CSCs) are among the target cells of cancer therapy because they are uniquely involved in both cancer progression and sensitivity to chemotherapeutic agents. We identified side population (SP) cells, which are known to be an enriched population of CSC, in five oral squamous cell carcinoma (OSCC) cells (SCC9, SCC25, TOSCC7, TOSCC17, and TOSCC23). The percentages of SP cells ranged from 0% to 3.3%, with TOSCC23 cells showing the highest percentages of SP cells (3.3% of the total cell population). The SP cells isolated from TOSCC23 cells also showed greater cell proliferation and invasion compared to non-SP (MP) cells. Therefore, our initial findings suggested that SP cells were enriched for CSC-like cells. Furthermore, DNA microarray analysis revealed that the expression of cell proliferation-related and anti-apoptotic genes was greater in SP cells compared to MP cells. We focused on Lin28a, which showed the highest expression (approximately 22-fold) among the upregulated genes. The overexpression of Lin28a in TOSCC23 cells increased their proliferation, colony formation, and invasion. These findings suggest that Lin28a is an appropriate CSC target molecule for OSCC treatment - Highlights: ► Lin28a is a SP cell-specific factor in oral squamous cell carcinoma (OSCC) cells. ► SP cells in OSCC cells show cancer stem cell-like properties. ► Lin28a regulates OSCC proliferative and invasive activities.

Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma

International Nuclear Information System (INIS)

Hayashi, S.; Tanaka, J.; Okada, S.; Isobe, T.; Yamamoto, G.; Yasuhara, R.; Irie, T.; Akiyama, C.; Kohno, Y.; Tachikawa, T.; Mishima, K.

2013-01-01

Cancer stem cells (CSCs) are among the target cells of cancer therapy because they are uniquely involved in both cancer progression and sensitivity to chemotherapeutic agents. We identified side population (SP) cells, which are known to be an enriched population of CSC, in five oral squamous cell carcinoma (OSCC) cells (SCC9, SCC25, TOSCC7, TOSCC17, and TOSCC23). The percentages of SP cells ranged from 0% to 3.3%, with TOSCC23 cells showing the highest percentages of SP cells (3.3% of the total cell population). The SP cells isolated from TOSCC23 cells also showed greater cell proliferation and invasion compared to non-SP (MP) cells. Therefore, our initial findings suggested that SP cells were enriched for CSC-like cells. Furthermore, DNA microarray analysis revealed that the expression of cell proliferation-related and anti-apoptotic genes was greater in SP cells compared to MP cells. We focused on Lin28a, which showed the highest expression (approximately 22-fold) among the upregulated genes. The overexpression of Lin28a in TOSCC23 cells increased their proliferation, colony formation, and invasion. These findings suggest that Lin28a is an appropriate CSC target molecule for OSCC treatment - Highlights: ► Lin28a is a SP cell-specific factor in oral squamous cell carcinoma (OSCC) cells. ► SP cells in OSCC cells show cancer stem cell-like properties. ► Lin28a regulates OSCC proliferative and invasive activities

Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.

Science.gov (United States)

He, Qin; Yang, Shuangyan; Gu, Xiuge; Li, Mengying; Wang, Chunling; Wei, Fulan

2018-04-19

Periodontal ligament stem cells (PDLSCs) are mesenchymal stem cells derived from dental tissues with multidirectional differentiation potential and excellent self-renewing ability. Recently, long noncoding RNAs (lncRNAs) have been shown to play important roles in MSC osteogenic differentiation. In this study, we found that taurine upregulated gene 1 (TUG1), an evolutionarily conserved and widely present lncRNA was significantly upregulated in osteogenically induced PDLSCs compared to their undifferentiated counterparts. Further investigation demonstrated that the expression of TUG1 was positively correlated with the osteogenic differentiation of PDLSCs following the induction, as evidenced by the increase in cellular alkaline phosphatase (ALP) level, formation of calcium nodules, and the upregulation of several osteogenic-related gene markers such as ALP, osteocalcin (OCN), and runt-related transcription factor 2 (Runx2). Conversely, TUG1 knockdown was demonstrated to inhibit the potential of PDLSCs for osteogenic differentiation. Using bioinformatics analysis, we identified lin-28 homolog A (Lin28A) as a potential target of TUG1 during osteogenic differentiation of PDLSCs. Lin28A was found to be significantly downregulated in TUG1-repressed PDLSCs and contained multiple binding sites for lncRNA TUG1. Moreover, suppression of Lin28A was shown to be able to inhibit osteogenic differentiation and decreased the expression of several osteogenic genes. Taken together, these results could help researchers better understand the mechanism that governs the osteogenic differentiation of PDLSCs, and also serve as a stepping stone for the development of novel therapeutic strategies that can be used to regenerate dental tissues.

Tip 2 Diabetes Mellituslu Hastalarda Mikroalbuminüri, İnsülin Direnci ve Kardiyovasküler Risk

OpenAIRE

ERSOY, Canan; TAŞLI, Babürşah; YILDIZ, Abdülmecit; İMAMOĞLU, Şazi

2006-01-01

Son çalışmalar tip 2 diyabette mikroalbuminüri (MAU) varlığı ile hedef organ hasarları ve kardiyovasküler hastalık riski arasındaki ilişkilere yönelmişlerdir. Bu çalışmada, Tip 2 diabetes mellituslu (DM) hastalarda MAU ile glisemik kontrol, insülin direnci (İR) ve diğer kardiyovasküler risk faktörleri arasındaki ilişki değerlendirilmiştir. İnsülin gereksinimi olmayan, normal renal fonksiyonlu 29 mikroalbuminürik ve 68 normoalbuminürik tip 2 DM'lu hasta çalışmaya dahil edildi. Tüm hast...

Properties of the fullerene C_6_0-containing PN Lin49 in the SMC; Explanations of strong near-IR excess

International Nuclear Information System (INIS)

Otsuka, Masaaki; Kemper, Francisca; Leal-Ferreira, Marcelo L.; Aleman, Isabel; Ochsendorf, Bram; Bernard-Salas, Jeronimo; Cami, Jan; Peeters, Els

2016-01-01

We performed a detailed spectroscopic analysis of the fullerene C_6o-containing planetary nebula (PN) Lin49 in the Small Magellanic Cloud (SMC). Lin49 is a C-rich and metal- deficient PN (Z∼⃒0.0006) and its nebular abundances are in agreement with the AGB model for the initially 1.25M_☉ stars with the metallicity Z = 0.001 of Fishlock et al. (2014, [1]). By stellar absorption fitting with TLUSTY, we derived stellar abundances, effective temperature, and surface gravity. We constructed the photo-ionization model with CLOUDY in order to investigate physical conditions of Lin49. The model with the 0.005-0.1 μm radius graphite and a constant hydrogen density shell could not fit the ∼⃒1-5 μm SED owing to the strong near-IR excess. We propose that the near-IR excess indicates (1) the presence of extremely small carbon molecules or (2) the presence of high-density structure surrounding the central star. (paper)

José Lins do Rego: um guia brasileiro de Israel

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Glauber Pereira Quintão

2010-03-01

Full Text Available Este artigo procura analisar, de forma crítica, o livro de José Lins do Rego, Roteiro de Israel, 1955. Busca-­se destacar duas facetas suplementares: a de um olhar poético e a de uma preocupação ético-política lançados pelo escritor sobre a construção do novo Estado de Israel. Observa-­se também uma relação entre os signos da modernidade e das histórias arcaica judaicas. Para isso, usa-­se o conceito de palimpsesto, de Gerard Genette, e de flâneur, de Walter Benjamim.

Synthèse et validation d'une loi de commande linéaire robuste de braquage d'une automobile

OpenAIRE

MENHOUR, Lghani; LECHNER, Daniel; CHARARA, Ali

2010-01-01

Ce papier propose la synthèse d'un retour d'état robuste préprogrammé pour le contrôle de direction d'un véhicule. Celui-ci est utilisé pour simuler le comportement du véhicule sous des fortes sollicitations dynamiques. Les contraintes d'inégalités matricielles linéaires (LMI) et un modèle linéaire à paramètres variants sont utilisés pour la synthèse du retour d'état. Ensuite, une méthode d'interpolation des gains est utilisée pour la conception d'un retour d'état robuste préprogrammé. La str...

Functional study of risk loci of stem cell-associated gene lin-28B and associations with disease survival outcomes in epithelial ovarian cancer.

Science.gov (United States)

Lu, Lingeng; Katsaros, Dionyssios; Mayne, Susan T; Risch, Harvey A; Benedetto, Chiara; Canuto, Emilie Marion; Yu, Herbert

2012-11-01

Several single-nucleotide polymorphisms (SNPs) of the stem cell-associated gene lin-28B have been identified in association with ovarian cancer and ovarian cancer-related risk factors. However, whether these SNPs are functional or might be potential biomarkers for ovarian cancer prognosis remains unknown. The purposes of this study were to investigate the functional relevance of the identified lin-28B SNPs, as well as the associations of genotype and phenotype with epithelial ovarian cancer (EOC) survival. We analyzed five SNPs and mRNA levels of lin-28B in 211 primary EOC tissues using Taqman(®) SNP genotyping assays and SYBR green-based real-time PCR, respectively. The RNA secondary structures at the region of a genome-wide association-identified intronic rs314276 were analyzed theoretically with mfold and experimentally with circular dichroism spectroscopy. We found that rs314276 was a cis-acting expression quantitative trait locus (eQTL) in both additive and dominant models, while rs7759938 and rs314277 were significant or of borderline significance in dominant models only. The rs314276 variant significantly affects RNA secondary structure. No SNPs alone were associated with patient survival. However, we found that among patients initially responding to chemotherapy, those with higher lin-28B expression had higher mortality risk (hazard ratio =3.27, 95% confidence interval: 1.63-6.56) and relapse risk (hazard ratio = 2.53, 95% confidence interval: 1.41-4.54) than those with lower expression, and these associations remained in multivariate analyses. These results suggest that rs314276 alters RNA secondary structure and thereby influences gene expression, and that lin-28B is a cancer stem cell-associated marker, which may be a pharmaceutical target in the management of EOC.

LEDWIRE: A Versatile Networking Platform for Smart LED Lighting Applications Using LIN-Bus and WSNs

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Dimitrios D. Piromalis

2016-05-01

Full Text Available In this paper, the architecture of a versatile networking and control platform for Light-Emitting Diode (LED lighting applications is presented, based on embedded wireless and wired networking technologies. All the possible power and control signals distribution topologies of the lighting fixtures are examined with particular focus on dynamic lighting applications with design metrics as the cost, the required wiring installation expenses and maintenance complexity. The proposed platform is optimized for applications where the grouping of LED-based lighting fictures clusters is essential, as well as their synchronization. With such an approach, the distributed control and synchronization of LED lighting fixtures' clusters is performed through a versatile network that uses the single wire Local Interconnect Network (LIN bus. The proposed networking platform is presented in terms of its physical layer architecture, its data protocol configuration, and its functionality for smart control. As a proof of concept, the design of a LED lighting fixture together with a LIN-to-IEEE802.15.4/ZigBee data gateway is presented.

"O Romancista e o Engenho: José Lins do Rego e o regionalismo nordestino dos anos 1920 e 1930", de Mariana Ghaguri "O Romancista e o Engenho: José Lins do Rego e o regionalismo nordestino dos anos 1920 e 1930", by Mariana Chaguri

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Mário Augusto Medeiros da Silva

2013-04-01

Full Text Available Trata-se das relações entre a formalização estética do Ciclo da Cana de Açúcar e o processo social do Regionalismo Nordestino. Analisa a trajetória social e produção literária de José Lins do Rego, buscando deslindar as formas de realização social do autor, nos circuitos intelectuais e políticos de que participou (Pernambuco e Rio de Janeiro, notadamente e as condições sociais de produção de sua obra (editoras em que a mesma é publicada, recepção entre a crítica de época etc.. Em vista das disputas travadas com os Modernistas paulistas de 1922, sua interpenetração com a crise política dos anos 1930 e com as formulações de Gilberto Freyre acerca das ideias de Região e Nação, Chaguri sugere a análise do Regionalismo Nordestino como parte de um projeto estético e político, cujas intenções e consequências merecem ser sociologicamente examinadas.The book deals with the relations between the aesthetics formalization of the Sugarcane Cycle and the social process of the Brazilian Northeastern regionalism. It analyzes the social trajectory and literary work of José Lins do Rego, seeking to disclose this author's ways of social fulfillment in the intellectual and political circles he participated (especially in Pernambuco and Rio de Janeiro as well as the social conditions of production of his work (his publishers, critiques etc.. Considering his disputes with members of the 1922 modernist movement, intertwined with the political crisis of the 1930s and the formulations of Gilberto Freyre's notions about Region and Nation, Chaguri suggests the analysis of Northeastern Regionalism while part of an aesthetic and political project whose intentions and consequences deserve to be sociologically examined.

An Adaptation of the Kernighan-Lin Heuristic to the Simple Graph Partitioning Problem

DEFF Research Database (Denmark)

Sørensen, Michael Malmros

1999-01-01

to this problem of the Kernighan-Lin exchange heuristic, which was originally developed for the closely related 2-partition problem. The evaluation is carried out on problem instances on graphs with up to 50 nodes for which the optimal partition values are known or upper bounds are available. The computational...... results show that among all instances with known optimal values the best partition values found by a randomized version of this heuristic lie well within 1% off the optimum....

Lin28a Is Dormant, Functional, and Dispensable During Mouse Oocyte-to-Embryo Transition

Czech Academy of Sciences Publication Activity Database

Flemr, Matyáš; Moravec, Martin; Libová, Veronika; Sedláček, Radislav; Svoboda, Petr

2014-01-01

Roč. 90, č. 6 (2014), s. 1-9 ISSN 0006-3363 R&D Projects: GA MŠk LH13084; GA MŠk(CZ) LM2011032; GA ČR(CZ) GBP305/12/G034 Institutional support: RVO:68378050 Keywords : early development * genome activation * guinea pigs * Let-7 * LIN28 * mice * microRNA * oocyte * rats * RNAi * rodents * transgenic/ knockout model * voles * zygote Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.318, year: 2014

Optical and electrical properties of zinc oxide thin films with low resistivity via Li-N dual-acceptor doping

Energy Technology Data Exchange (ETDEWEB)

Zhang Daoli, E-mail: zhang_daoli@mail.hust.edu.cn [Department of Electronic Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China); Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China); Zhang Jianbing [Department of Electronic Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China); Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China); Guo Zhe [Department of Electronic Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China); Miao Xiangshui [Department of Electronic Science and Technology, Huazhong University of Science and Technology, No. 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China); Wuhan National Laboratory for Optoelectronics, 1037 Luoyu Road, Hongshan District, Wuhan City, Hubei Province 430074 (China)

2011-05-19

Highlights: > Zinc oxide films have been deposited on glass substrates by Li-N dual-acceptor doping method via a modified SILAR method. > The resistivity of ZnO film was found to be 1.04 {Omega} cm with a Hall mobility of 0.749 cm{sup 2} V{sup -1} s{sup -1}, carrier concentration of 8.02 x 1018 cm{sup -3}, and transmittance of about 80% in visible range showing good crystallinity with prior c-axis orientation. > A shallow acceptor level of 91 meV is identified from free-to-neutral-acceptor transitions. > Another deep level of 255 meV was ascribed to Li{sub Zn}-Li{sub i} complex. - Abstract: Zinc oxide thin films with low resistivity have been deposited on glass substrates by Li-N dual-acceptor doping method via a modified successive ionic layer adsorption and reaction process. The thin films were systematically characterized via scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction, ultraviolet-visible spectrophotometry and fluorescence spectrophotometry. The resistivity of zinc oxide film was found to be 1.04 {Omega} cm with a Hall mobility of 0.749 cm{sup 2} V{sup -1} s{sup -1} and carrier concentration of 8.02 x 10{sup 18} cm{sup -3}. The Li-N dual-acceptor doped zinc oxide films showed good crystallinity with prior c-axis orientation, and high transmittance of about 80% in visible range. Moreover, the effects of Li doping level and other parameters on crystallinity, electrical and ultraviolet emission of zinc oxide films were investigated.

Lin- CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitors.

Science.gov (United States)

Dahlin, Joakim S; Malinovschi, Andrei; Öhrvik, Helena; Sandelin, Martin; Janson, Christer; Alving, Kjell; Hallgren, Jenny

2016-01-28

Mast cells are rare tissue-resident immune cells that are involved in allergic reactions, and their numbers are increased in the lungs of asthmatics. Murine lung mast cells arise from committed bone marrow-derived progenitors that enter the blood circulation, migrate through the pulmonary endothelium, and mature in the tissue. In humans, mast cells can be cultured from multipotent CD34(+) progenitor cells. However, a population of distinct precursor cells that give rise to mast cells has remained undiscovered. To our knowledge, this is the first report of human lineage-negative (Lin(-)) CD34(hi) CD117(int/hi) FcεRI(+) progenitor cells, which represented only 0.0053% of the isolated blood cells in healthy individuals. These cells expressed integrin β7 and developed a mast cell-like phenotype, although with a slow cell division capacity in vitro. Isolated Lin(-) CD34(hi) CD117(int/hi) FcεRI(+) blood cells had an immature mast cell-like appearance and expressed high levels of many mast cell-related genes as compared with human blood basophils in whole-transcriptome microarray analyses. Furthermore, serglycin, tryptase, and carboxypeptidase A messenger RNA transcripts were detected by quantitative reverse transcription-polymerase chain reaction. Altogether, we propose that the Lin(-) CD34(hi) CD117(int/hi) FcεRI(+) blood cells are closely related to human tissue mast cells and likely constitute an immediate precursor population, which can give rise to predominantly mast cells. Furthermore, asthmatics with reduced lung function had a higher frequency of Lin(-) CD34(hi) CD117(int/hi) FcεRI(+) blood mast cell progenitors than asthmatics with normal lung function. © 2016 by The American Society of Hematology.

Initial results on computational performance of Intel Many Integrated Core (MIC) architecture: implementation of the Weather and Research Forecasting (WRF) Purdue-Lin microphysics scheme

Science.gov (United States)

Mielikainen, Jarno; Huang, Bormin; Huang, Allen H.

2014-10-01

Purdue-Lin scheme is a relatively sophisticated microphysics scheme in the Weather Research and Forecasting (WRF) model. The scheme includes six classes of hydro meteors: water vapor, cloud water, raid, cloud ice, snow and graupel. The scheme is very suitable for massively parallel computation as there are no interactions among horizontal grid points. In this paper, we accelerate the Purdue Lin scheme using Intel Many Integrated Core Architecture (MIC) hardware. The Intel Xeon Phi is a high performance coprocessor consists of up to 61 cores. The Xeon Phi is connected to a CPU via the PCI Express (PICe) bus. In this paper, we will discuss in detail the code optimization issues encountered while tuning the Purdue-Lin microphysics Fortran code for Xeon Phi. In particularly, getting a good performance required utilizing multiple cores, the wide vector operations and make efficient use of memory. The results show that the optimizations improved performance of the original code on Xeon Phi 5110P by a factor of 4.2x. Furthermore, the same optimizations improved performance on Intel Xeon E5-2603 CPU by a factor of 1.2x compared to the original code.

Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B.

Science.gov (United States)

Lee, Chih-Yung Sean; Lu, Tu; Seydoux, Geraldine

2017-11-07

Nanos RNA-binding proteins are required for germline development in metazoans, but the underlying mechanisms remain poorly understood. We have profiled the transcriptome of primordial germ cells (PGCs) lacking the nanos homologs nos-1 and nos-2 in C. elegans. nos-1nos-2 PGCs fail to silence hundreds of transcripts normally expressed in oocytes. We find that this misregulation is due to both delayed turnover of maternal transcripts and inappropriate transcriptional activation. The latter appears to be an indirect consequence of delayed turnover of the maternally-inherited transcription factor LIN-15B, a synMuvB class transcription factor known to antagonize PRC2 activity. PRC2 is required for chromatin reprogramming in the germline, and the transcriptome of PGCs lacking PRC2 resembles that of nos-1nos-2 PGCs. Loss of maternal LIN-15B restores fertility to nos-1nos-2 mutants. These findings suggest that Nanos promotes germ cell fate by downregulating maternal RNAs and proteins that would otherwise interfere with PRC2-dependent reprogramming of PGC chromatin.

Traitement statistique des distorsions non-linéaires pour la restauration des enregistrements sonores.

OpenAIRE

Picard , Guillaume

2006-01-01

L'objet de la thèse est l'étude, la modélisation et le traitement des distorsions non linéaires sonores, pour lesquelles les techniques actuelles s'avèrent impuissantes. L'approche retenue consiste à représenter, globalement, à la fois le signal audio à restaurer et le processus de distorsion, dans le cadre d'un modèle statistique. Cette approche présente un bon compromis entre une souhaitable généricité -possibilité de traiter à l'aide d'une méthode globale plusieurs types de distorsions- et...

Reduction of EMC Emissions in Mixed Signal Integrated Circuits with Embedded LIN Driver

Directory of Open Access Journals (Sweden)

P. Hartl

2016-06-01

Full Text Available This paper describes several methods for reduction of electromagnetic emissions (EME of mixed signal integrated circuits (IC. The focus is on the impact that a LIN bus communication block has on a complex IC which contains analog blocks, noisy digital block, micro-core (µC and several types of memories. It is used in an automotive environment, where EMC emission reduction is one of the key success factors. Several proposed methods for EME reduction are described and implemented on three test chips. These methods include current consumption reduction, internal on-chip decoupling, ground separation and different linear voltage regulator topologies. Measurement results of several fabricated test chips are shown and discussed.

Applying a Multiple Screening Program Aided by a Guideline-driven Computerized Decision Support System—A Pilot Experience in Yun-Lin, Taiwan

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Jou-Wei Lin

2007-01-01

Conclusion: A computer-aided screening program driven by the US Preventive Services Task Force recommendations has been successfully implemented in Yun-Lin, Taiwan, and provided useful information about local epidemiology and implications for future health policy making. [J Formos Med Assoc 2007; 106(1:58-68

[Heavy metal pollution characteristics and ecological risk analysis for soil in Phyllostachys praecox stands of Lin'an].

Science.gov (United States)

Fang, Xiao-bo; Shi, Han; Liao, Xin-feng; Lou, Zhong; Zhou, Lyu-yan; Yu, Hai-xia; Yao, Lin; Sun, Li-ping

2015-06-01

An investigation was carried out in an attempt to reveal the characteristics of heavy metals contamination in the soils of Phyllostachys praecox forest in Lin' an. Based on the concentrations of Hg, As, Cu, Pb, Zn, Cd, Cr, Ni, Co and Mn in 160 topsoil samples, the pollution status and ecological risks of heavy metals in the soils were assessed by single factor pollution index, Nemerow integrated pollution index and Hankanson potential ecological risk index. The spatial variability of heavy metal concentrations in the soils closely related to the distribution of traffic, industrial and livestock pollution sources. The average concentrations of Hg, As, Cu, Pb, Zn, Cd, Cr, Ni, Co and Mn in the soils were 0.16, 7.41, 34.36, 87.98, 103.98, 0.26, 59.12, 29.56, 11.44 and 350.26 mg · kg(-1), respectively. Pb, Cd, Zn and Cu concentrations were as 2.89, 1.70, 1.12 and 1.12 times as the background values of soil in Zhejiang Province, respectively. But their concentrations were all lower than the threshold values of the National Environmental Quality Standard for Soil (GB 15618-1995). The average single factor pollution index revealed that the level of heavy metal pollution in the soils was in order of Pb>Cd>Cu= Zn>Hg>As>Ni>Co>Cr>Mn. Pb pollution was of moderate level while Cd, Cu and Zn pollutions were slight. There was no soil pollution caused by the other heavy metals. However, the Nemerow integrated pollution index showed that all the 160 soil samples were contaminated by heavy metals to a certain extent. Among total 160 soil samples, slight pollution level, moderate pollution level and heavy pollution level accounted for 55.6%, 29.4% and 15.0%, respectively. The average single factor potential ecological risk index (Er(i)) implied that the potential ecological risk related to Cd reached moderate level, while the others were of slight level. Furthermore, Cd and Hg showed higher potential ecological risk indices which reached up to 256.82 and 187.33 respectively

A MULHER NEGRA E AS RELAÇÕES DE GÊNERO EM MENINO DE ENGENHO DE JOSÉ LINS DO REGO THE BLACK WOMAN AND GENDER RELATIONS IN MENINO DE ENGENHO BY JOSÉ LINS DO REGO

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Zélia Monteiro Bora ; Marina Rodrigues de Oliveira

2011-02-01

Full Text Available Os romances do ciclo da cana-de-açúcar, do escritor paraibano José Lins do Rego (1901-1957 e a sua relação com a sociedade e a cultura destacam-se enquanto abordagens ficcionais, através das quais o escritor buscou representar aspectos relevantes para o entendimento das relações de gênero no Nordeste, no começo do século vinte. Tal representação, indiscutivelmente, reflete a perspectiva de um narrador profundamente marcado pelo lugar privilegiado de onde narra e de sua condição existencial, como o neto de um senhor de engenho. Diante desses aspectos, propomo- nos a analisar brevemente as implicações dessa perspectiva sobre a construção simbólica da personagem negra representada no romance. Para tanto, serão utilizados como referenciais críticos, além do citado romance, os estudos de Zagury (1982, Albuquerque Jr. (1999, Azevedo (1996, 2007, Freyre (2006 Chaguri (2009, Schwarz (2008 e Raboni (2010.The so called sugar cane cycle novels by the Parahyban writer Jose Lins do Rego (1901-197 and its relationship with society and culture constitute themselves as fictional approaches through which the writer sought to represent aspects to the understanding of gender relations in the Northeast of Brazil in the early twentieth century. Such representation, undoubtedly reflects the perspective of a narrator deeply marked by the privileged place from which he narrates and his existential condition, as the son of a plantation owner. Given these aspects, we propose to examine briefly the implications of his perspective on the symbolic construction of the black woman characteres represented in the novel. The following

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry▿

OpenAIRE

Monincová, Marta; Prokop, Zbyněk; Vévodová, Jitka; Nagata, Yuji; Damborský, Jiří

2007-01-01

1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s−1) of Li...

An attempt of using soil grain size in calculating the capacity of water unavailable to plants / Próba wykorzystania uziarnienia gleby do obliczania zawartości wody niedostępnej dla roślin

Directory of Open Access Journals (Sweden)

Brogowski Zygmunt

2015-03-01

Full Text Available Celem pracy była próba znalezienia możliwości obliczenia ilości wody niedostępnej dla roślin na podstawie uziarnienia gleb. Frakcje o średnicach <0,02 mm gromadzą od 90,1 do 98,8% całkowitej ilości wody niedostępnej dla roślin, a frakcja iłu koloidalnego (<0,002 mm zatrzymuje od 52,1 do 80,1% tej wody. Gromadzenie wody we frakcjach <0,02 mm znacząco zależy od ich składu mineralogicznego. Obecność we frakcjach illitu, chlorytu oraz kaolinitu powoduje zmniejszenie ilości wody niedostępnej nawet o 40% w stosunku do frakcji zbudowanych ze smektytów, wermikulitów oraz próchnicy. Ze względu na duże zróżnicowanie ilości wody niedostępnej dla roślin w poszczególnych frakcjach granulometrycznych <0,02 mm wynikaj¹ce ze zróżnicowania mineralogicznego tych frakcji nie udało się wyprowadzić współczynników pozwalających na obliczenie ilościowe w glebach wody niedostępnej dla roślin wiązanej w mikroporach <0,2 μm.

The Many Sides of Happy Lim: aka Hom Ah Wing, Lin Jian Fu, Happy Lum, Lin Chien Fu, Hom Yen Chuck, Lam Kin Foo, Lum Kin Foo, Hom, Lim Goon Wing, Lim Gin Foo, Gin Foo Lin, Koon Wing Lim, Henry Chin, Lim Ying Chuck, Lim Ah Wing, et. al.

Directory of Open Access Journals (Sweden)

Gordon H. Chang

2010-03-01

Full Text Available

Known to the FBI, INS, and IRS as Mr. Ah Wing Hom, he was also Lin Jian Fu, Jian Fu (Tough Guy, or just Fu to the readers of the many poems and short stories he published in Chinese over four decades in the twentieth century. And to still others, primarily English-readers of his writing, he was Happy Lim, an ironic, even tragically bizarre name, as his life was far from pleasant. On New Year's Day 1986, he died alone in a dingy San Francisco Chinatown bachelor hotel suffering from a bacterial infection and a chronic blood disorder that had required the amputation of all his toes the year before. He was seventy-eight years of age and had spent most of his life within blocks of where he died.

Transport properties of gaseous ions over a wide energy range. Part III

International Nuclear Information System (INIS)

Ellis, H.W.; Thackston, M.G.; McDaniel, E.W.; Mason, E.A.

1984-01-01

This paper updates and extends in scope our two previous papers entitled ''Transport Properties of Gaseous Ions over a Wide Energy Range.'' The references to the earlier publications (referred to as ''Part I'' and ''Part II'') are I, H. W. Ellis, R. Y. Pai, E. W. McDonald, E. A. Mason, and L. A. Viehland, ATOMIC DATA AND NUCLEAR DATA TABLES 17, 177--210 (19876); and II, H. W. Ellis, E. W. McDaniel, D. L. Albritton, L. A. Veihland, S. L. Lin, and E. A. Mason, ATOMIC DATA AND NUCLEAR DATA TABLES 22, 179--217 (1978). Parts I and II contained compilations of experimental data on ionic mobilities and diffusion coefficients (both longitudinal and transverse) for ions in neutral gase (almost exclusively at room temperature) in an externally applied electric field

Efeito e modo de ação das bacteriocinas produzidas por Lactococcus lactis subsp. lactis ITAL 383, ATCC 11454 e CNRZ 150 contra Listeria innocua LIN 11 Effect and mode of action of the bacterioncin produced by Lactococcus. lactis subsp. lactis ITAL 383, ATCC 11454 e CNRZ 150 against Listeria innocua LIN 11

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Izildinha MORENO

1999-01-01

Full Text Available O efeito e o modo de ação das bacteriocinas produzidas por L. lactis subsp. lactis ITAL 383 e CNRZ 150 são similares à nisina de L. lactis subsp. lactis ATCC 11454. Estas bacteriocinas apresentaram um modo de ação bactericida, causando a lise de células de L. innocua LIN 11, associada ao decréscimo da absorbância e da viabilidade celular. O efeito letal foi maior para células em fase exponencial comparativamente à fase estacionária de crescimento. A adsorção dessas bacteriocinas às células de L. innocua LIN 11 foi muito rápida e influenciada pelo pH do meio de suspensão; adsorção máxima foi verificada a pH 6,0 e logo após o contato inicial. Perda completa de adsorção ocorreu em pH 2,0.The effect and mode of action of the bacteriocin produced by L. lactis subsp. lactis ITAL 383 and CNRZ 150 are similar to the nisin produced by L. lactis subsp. lactis ATCC 11454. It was clearly bactericidal, and caused lysis of a strain of L. innocua LIN 11 detected by the decrease of absorbance values and the cell viability. Their lethal effect was considerably higher during the logarithmic growth when compared to the stationary phase. Adsorption developed rapidly and was influenced by the pH value of the suspension medium. Maximum adsorption was observed at pH 6,0 and immediately after initial contact and loss at pH 2,0.

Evidence of cation vacancy induced room temperature ferromagnetism in Li-N codoped ZnO thin films

Science.gov (United States)

Zhang, B. Y.; Yao, B.; Li, Y. F.; Liu, A. M.; Zhang, Z. Z.; Li, B. H.; Xing, G. Z.; Wu, T.; Qin, X. B.; Zhao, D. X.; Shan, C. X.; Shen, D. Z.

2011-10-01

Room temperature ferromagnetism (RTFM) was observed in Li-N codoped ZnO thin films [ZnO:(Li, N)] fabricated by plasma-assisted molecular beam epitaxy, and p-type ZnO:(Li, N) shows the strongest RTFM. Positron annihilation spectroscopy and low temperature photoluminescence measurements indicate that the RTFM in ZnO:(Li, N) is attributed to the defect complex related to VZn, such as VZn and Lii-NO-VZn complex, well supported by first-principles calculations. The incorporation of NO can stabilize and enhance the RTFM of ZnO:(Li, N) by combining with Lii to form Lii-NO complex, which restrains the compensation of Lii for VZn and makes the ZnO:(Li, N) conduct in p-type.

Search for the signature of a halo structure in the p(6He,6Li)n reaction

International Nuclear Information System (INIS)

Cortina-Gil, M.D.; Roussel-Chomaz, P.; Mittig, W.; Casandjian, J.M.; Chartier, M.; Alamanos, N.; Auger, F.; Fekou-Youmbi, V.; Blumenfeld, Y.; and others.

1995-01-01

The elastic scattering p( 6 He, 6 He)p and charge exchange reaction p( 6 He, 6 Li)n have been measured in reverse kinematics with a secondary 6 He beam. The angular distributions for these reactions were obtained. In the case of the charge exchange reaction, the ratio of the cross section for the Gamow-Teller transition to the ground state, and for the Fermi transition to the isobaric analog state is a measure of the relative strength of the two components of the exchange interaction. This ratio is found compatible with existing systematics for stable T=1 nuclei, and no clear signature of a halo structure was found in the present data. (author)

Quando "falha a fala" e "fala a bala": lingua(gem e violência em Cidade de Deus, de Paulo Lins

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Rita Gabrielli Pereira

2018-04-01

Full Text Available Cidade de Deus traz em si a história da construção do conjunto habitacional, cujo nome intitula o romance, e de sua transformação em uma das favelas mais violentas do Rio de Janeiro, por meio do entrecruzamento das histórias de Cabeleira, Bené, Zé Pequeno e Busca-Pé. Concebendo o romance, nos atos da escrita e da leitura, como uma enunciação (BENVENISTE, 1989a; 1989b; 1995a; 1995b entre autor e leitor, constituída por outras enunciações — entre narrador e narratário e entre personagens — a partir do "ato ficcional" (ISER, 2002 do autor, vamos nos guiar por essa tessitura de/entre histórias para rastrear o modo como Lins relaciona linguagem e violência. Palavras-chave: Lingua(gem, Subjetividade, Violência ______________________________ Cidade de Deus tells the history of the construction of the housing complex that entitles the novel and its transformation process into one of the most violent slums of Rio de Janeiro. By treating the novel as an enunciation (BENVENISTE, 1989a; 1989b; 1995a; 1995b between author and reader, which is made of other enunciations − between narrator and narratee and between characters −, based on the author´s “fictional act”(ISER, 2002, we intend to analyze the way how Lins relates language and violence. Keywords: Language, Subjectivity, Violence.

Evidence of cation vacancy induced room temperature ferromagnetism in Li-N codoped ZnO thin films

International Nuclear Information System (INIS)

Zhang, B. Y.; Yao, B.; Li, Y. F.; Xing, G. Z.; Liu, A. M.; Zhang, Z. Z.; Li, B. H.; Zhao, D. X.; Shan, C. X.; Shen, D. Z.; Wu, T.; Qin, X. B.

2011-01-01

Room temperature ferromagnetism (RTFM) was observed in Li-N codoped ZnO thin films [ZnO:(Li, N)] fabricated by plasma-assisted molecular beam epitaxy, and p-type ZnO:(Li, N) shows the strongest RTFM. Positron annihilation spectroscopy and low temperature photoluminescence measurements indicate that the RTFM in ZnO:(Li, N) is attributed to the defect complex related to V Zn , such as V Zn and Li i -N O -V Zn complex, well supported by first-principles calculations. The incorporation of N O can stabilize and enhance the RTFM of ZnO:(Li, N) by combining with Li i to form Li i -N O complex, which restrains the compensation of Li i for V Zn and makes the ZnO:(Li, N) conduct in p-type.

''SMILE'': A Self Magnetically Insulated Transmission LinE adder for the 8-stage RADLAC II accelerator

International Nuclear Information System (INIS)

Mazarakis, M.G.; Poukey, J.W.; Shope, S.L.; Frost, C.A.; Turman, B.N.; Ramirez, J.J.; Prestwich, K.R.; Pankuch, P.J.

1991-01-01

The RADLAC II Self Magnetically Insulated Transmission LinE ''SMILE'' is a coaxial wave guide structure that is composed of two regions: (a) a 9.5-m voltage adder and (b) a 3-m long extension section. The adder section provides for the addition of the input voltages from the individual water-dielectric pulse forming line feeds. The extension section isolates the adder from the magnetically immersed foilless diode electron source load and efficiently transports the pulsed power out from the deionized water tank of the device. The SMILE modification of the RADLAC II accelerator enabled us to produce high quality beams of up to 14 MV, 100 kA. The design and the experimental evaluation of SMILE will be presented and compared with numerical simulation predictions. 12 refs., 9 figs., 1 tab

Qualidade de um sistema latossolo-argissolo como receptor de efluentes no município de Lins(SP Quality of a latosol-argisol system in the county of Lins/SP, Brazil

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Liliane Ibrahim

2008-12-01

Full Text Available Este trabalho objetivou caracterizar um sistema de solos, evidenciando a propriedades que possam esclarecer sua dinâmica e contribuir para a definição de critérios que condicionem a aptidão destes solos como receptores de efluentes. Trata-se de uma área experimental de estudos, contígua à Estação de Tratamento de Esgoto do município de Lins (SP, onde o efluente é gerado a partir do tratamento de esgoto por sistema de lagoas de estabilização. Os solos, situados ao longo de uma vertente com ligeira inclinação, foram caracterizados por meio de análises, morfológica, granulométrica, química, mineralógica e micromorfológica, realizadas em amostras coletadas em cinco trincheiras em toposseqüência. Os solos são desenvolvidos a partir dos sedimentos arenosos da Formação Adamantina (Grupo Bauru e constituem um sistema Latossolo - Argissolo onde a transição Bw - Bt ocorre lateralmente do topo para a base da vertente. Foram identificadas três fases pedogenéticas nesta associação de solos. A primeira, argiluviação e adensamento de partículas, responsável pela formação dos horizontes texturais, foi superposta pelos processos de latossolização e hidromorfismo, atuantes na dinâmica atual destes solos. Análises micromorfológicas mostraram tratar-se de solos com intensa porosidade, caracterizada pelo empilhamento dos grãos do esqueleto quartzoso amplamente predominante e pelo arranjo entre os microagregados granulares. A permeabilidade é ainda favorecida pela intensa ação da mesofauna. Os solos são distróficos e compostos por caulinita e óxidos de Fe na fração argilosa. Por constituírem um sistema frágil, a disposição de quaisquer tipos de resíduos nestes solos requer o monitoramento constante de suas propriedades, tanto para a manutenção, quanto para a recuperação da qualidade desta cobertura pedológica.The purpose of this study was the characterization of a soil system, focused on proprieties that

Regio- and Enantioselective Sequential Dehalogenation of rac-1,3-Dibromobutane by Haloalkane Dehalogenase LinB.

Science.gov (United States)

Gross, Johannes; Prokop, Zbyněk; Janssen, Dick; Faber, Kurt; Hall, Mélanie

2016-08-03

The hydrolytic dehalogenation of rac-1,3-dibromobutane catalyzed by the haloalkane dehalogenase LinB from Sphingobium japonicum UT26 proceeds in a sequential fashion: initial formation of intermediate haloalcohols followed by a second hydrolytic step to produce the final diol. Detailed investigation of the course of the reaction revealed favored nucleophilic displacement of the sec-halogen in the first hydrolytic event with pronounced R enantioselectivity. The second hydrolysis step proceeded with a regioselectivity switch at the primary position, with preference for the S enantiomer. Because of complex competition between all eight possible reactions, intermediate haloalcohols formed with moderate to good ee ((S)-4-bromobutan-2-ol: up to 87 %). Similarly, (S)-butane-1,3-diol was formed at a maximum ee of 35 % before full hydrolysis furnished the racemic diol product. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Comparability of estimating energy rebound effect should be based on uniform mechanism and benchmark: A reply to Du and Lin

International Nuclear Information System (INIS)

Li, Chengyu; Shao, Shuai; Yang, Lili; Yu, Mingliang

2016-01-01

Du and Lin (2015) argued that the estimation model of the economy-wide energy rebound effect proposed by Shao et al. (2014) should be revised and provided an alternative approach, which they considered to be more consistent with the definition of the rebound effect. However, in this comment, we do not find a valid correction or modification to our original model, because their criticism logic does not originate from the corresponding mechanism in Shao et al. (2014), and their estimation formula has a different benchmark with ours. Moreover, their data samples were also different from ours, generating the incomparable results, and there are some irrational results in the comment. Even based on different estimation formulas in the two studies and using the same estimation method and data sample, the comparison results show that the problem of the estimation formula in our previous study which they claimed does not really exist. We argue that this comment is not consistent with the principle of the rebound effect. Actually, their work can be only regarded as proposing an alternative approach for the estimate of the rebound effect. Therefore, their argument is not enough to overturn our previous study. - Highlights: • A reply to Du and Lin (2015), who questioned our previous study, is provided. • Their criticism logic does not originate from our corresponding mechanism. • Their estimation formula has a different benchmark with ours. • Different data samples in the two papers make their results incomparable. • Their argument is not enough to overturn our previous study.

Solving quantum optimal control problems using Clebsch variables and Lin constraints

Science.gov (United States)

Delgado-Téllez, M.; Ibort, A.; Rodríguez de la Peña, T.

2018-01-01

Clebsch variables (and Lin constraints) are applied to the study of a class of optimal control problems for affine-controlled quantum systems. The optimal control problem will be modelled with controls defined on an auxiliary space where the dynamical group of the system acts freely. The reciprocity between both theories: the classical theory defined by the objective functional and the quantum system, is established by using a suitable version of Lagrange’s multipliers theorem and a geometrical interpretation of the constraints of the system as defining a subspace of horizontal curves in an associated bundle. It is shown how the solutions of the variational problem defined by the objective functional determine solutions of the quantum problem. Then a new way of obtaining explicit solutions for a family of optimal control problems for affine-controlled quantum systems (finite or infinite dimensional) is obtained. One of its main advantages, is the the use of Clebsch variables allows to compute such solutions from solutions of invariant problems that can often be computed explicitly. This procedure can be presented as an algorithm that can be applied to a large class of systems. Finally, some simple examples, spin control, a simple quantum Hamiltonian with an ‘Elroy beanie’ type classical model and a controlled one-dimensional quantum harmonic oscillator, illustrating the main features of the theory, will be discussed.

Evidence of cation vacancy induced room temperature ferromagnetism in Li-N codoped ZnO thin films

Energy Technology Data Exchange (ETDEWEB)

Zhang, B. Y. [Key Laboratory of Excited State Process, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Science, Changchun 130033 (China); School of Physics and Optoelectronic Technology, Dalian University of Technology, Dalian 116023 (China); Yao, B. [Key Laboratory of Excited State Process, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Science, Changchun 130033 (China); State Key Laboratory of Superhard Material, Department of Physics, Jilin University, Changchun 130023 (China); Li, Y. F.; Xing, G. Z. [State Key Laboratory of Superhard Material, Department of Physics, Jilin University, Changchun 130023 (China); Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore); Liu, A. M. [School of Physics and Optoelectronic Technology, Dalian University of Technology, Dalian 116023 (China); Zhang, Z. Z.; Li, B. H.; Zhao, D. X.; Shan, C. X.; Shen, D. Z. [Key Laboratory of Excited State Process, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Science, Changchun 130033 (China); Wu, T. [Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore); Qin, X. B. [Key Laboratory of Nuclear Analysis Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049 (China)

2011-10-31

Room temperature ferromagnetism (RTFM) was observed in Li-N codoped ZnO thin films [ZnO:(Li, N)] fabricated by plasma-assisted molecular beam epitaxy, and p-type ZnO:(Li, N) shows the strongest RTFM. Positron annihilation spectroscopy and low temperature photoluminescence measurements indicate that the RTFM in ZnO:(Li, N) is attributed to the defect complex related to V{sub Zn}, such as V{sub Zn} and Li{sub i}-N{sub O}-V{sub Zn} complex, well supported by first-principles calculations. The incorporation of N{sub O} can stabilize and enhance the RTFM of ZnO:(Li, N) by combining with Li{sub i} to form Li{sub i}-N{sub O} complex, which restrains the compensation of Li{sub i} for V{sub Zn} and makes the ZnO:(Li, N) conduct in p-type.

Cartographie des linéaments géologiques en domaine aride par extraction semi-automatique à partir d’images satellitaires: Exemple à la région d’El Kseïbat (Sahara algérien

Directory of Open Access Journals (Sweden)

Hammad, N.

2016-06-01

Full Text Available Geologists in charge of a detailed lineament mapping in arid and desert area, face the extent of the land and the abundance of eolian deposits. This study presents a semi-automatic approach of extraction of lineament, different from other methods, such as the automatic extraction and manual extraction, by being both fast and objective. It consists of a series of digital processing (textural and spatial filtering, binarization by thresholding and mathematic morphology ... etc. applied to a Landsat 7 ETM+scene. This semi-automatic approach has produced a detailed map of lineaments, while taking account of tectonic directions recognized in the region. It helps mitigate the effect of dune deposits meet the specifications of arid environment. The visual validation of these linear structures, by geoscientists and field data, allowed the identification of the majority of structural lineaments or at least those tried geological.[fr] Les géologues en charge d’une cartographie des linéaments détaillée en domaine aride et désertique, font face à l’étendue des terrains et la présence abondante de dépôts éoliens. La présente étude présente une approche semi-automatique différente des autres méthodes d’extraction des linéaments, à savoir l’extraction automatique et l’extraction manuelle par le fait d’être à la fois rapide et objective. Elle consiste en une succession de traitements numériques (filtrage textural et spatial, binarisation par seuillage et morphologie mathématique…etc. appliqués sur une scène LANDSAT-7 ETM+. Cette approche semi-automatique a permis d’obtenir une carte détaillée des linéaments, tout en tenant compte des directions tectoniques reconnues dans la région. Elle permet d’atténuer l’effet des dépôts dunaires répondant aux spécifications du contexte aride. La validation visuelle de ces structures linéaires, effectuée sur terrain et sur la base de la carte géologique et des données g

QUANTUM INSPIRED PARTICLE SWARM COMBINED WITH LIN-KERNIGHAN-HELSGAUN METHOD TO THE TRAVELING SALESMAN PROBLEM

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Bruno Avila Leal de Meirelles Herrera

2015-12-01

Full Text Available ABSTRACT The Traveling Salesman Problem (TSP is one of the most well-known and studied problems of Operations Research field, more specifically, in the Combinatorial Optimization field. As the TSP is a NP (Non-Deterministic Polynomial time-hard problem, there are several heuristic methods which have been proposed for the past decades in the attempt to solve it the best possible way. The aim of this work is to introduce and to evaluate the performance of some approaches for achieving optimal solution considering some symmetrical and asymmetrical TSP instances, which were taken from the Traveling Salesman Problem Library (TSPLIB. The analyzed approaches were divided into three methods: (i Lin-Kernighan-Helsgaun (LKH algorithm; (ii LKH with initial tour based on uniform distribution; and (iii an hybrid proposal combining Particle Swarm Optimization (PSO with quantum inspired behavior and LKH for local search procedure. The tested algorithms presented promising results in terms of computational cost and solution quality.

Cell type-specific recruitment of Drosophila Lin-7 to distinct MAGUK-based protein complexes defines novel roles for Sdt and Dlg-S97.

Science.gov (United States)

Bachmann, André; Timmer, Marco; Sierralta, Jimena; Pietrini, Grazia; Gundelfinger, Eckart D; Knust, Elisabeth; Thomas, Ulrich

2004-04-15

Stardust (Sdt) and Discs-Large (Dlg) are membrane-associated guanylate kinases (MAGUKs) involved in the organization of supramolecular protein complexes at distinct epithelial membrane compartments in Drosophila. Loss of either Sdt or Dlg affects epithelial development with severe effects on apico-basal polarity. Moreover, Dlg is required for the structural and functional integrity of synaptic junctions. Recent biochemical and cell culture studies have revealed that various mammalian MAGUKs can interact with mLin-7/Veli/MALS, a small PDZ-domain protein. To substantiate these findings for their in vivo significance with regard to Sdt- and Dlg-based protein complexes, we analyzed the subcellular distribution of Drosophila Lin-7 (DLin-7) and performed genetic and biochemical assays to characterize its interaction with either of the two MAGUKs. In epithelia, Sdt mediates the recruitment of DLin-7 to the subapical region, while at larval neuromuscular junctions, a particular isoform of Dlg, Dlg-S97, is required for postsynaptic localization of DLin-7. Ectopic expression of Dlg-S97 in epithelia, however, was not sufficient to induce a redistribution of DLin-7. These results imply that the recruitment of DLin-7 to MAGUK-based protein complexes is defined by cell-type specific mechanisms and that DLin-7 acts downstream of Sdt in epithelia and downstream of Dlg at synapses.

Expression and associations of TRAF1, BMI-1, ALDH1, and Lin28B in oral squamous cell carcinoma.

Science.gov (United States)

Wu, Tian-Fu; Li, Yi-Cun; Ma, Si-Rui; Bing-Liu; Zhang, Wen-Feng; Sun, Zhi-Jun

2017-04-01

Tumor necrosis factor receptor-associated factor 1, an adaptor protein of tumor necrosis factor 2, is involved in classical nuclear factor (NF)-κB activation and lymphocyte recruitment. However, less is known about the expression and association of tumor necrosis factor receptor-associated factor 1 with cancer stem cell markers in oral squamous cell carcinoma. This study aimed to investigate the expression of tumor necrosis factor receptor-associated factor 1 and stem cell characteristic markers (lin28 homolog B, B cell-specific Moloney murine leukemia virus integration site 1, and aldehyde dehydrogenase 1) in oral squamous cell carcinoma and analyze their relations. Paraffin-embedded tissues of 78 oral squamous cell carcinomas, 39 normal oral mucosa, and 12 oral dysplasia tissues were employed in tissue microarrays, and the expression of tumor necrosis factor receptor-associated factor 1, B cell-specific Moloney murine leukemia virus integration site 1, aldehyde dehydrogenase 1, and lin28 homolog B was measured by immunohistostaining and digital pathological analysis. The expression of tumor necrosis factor receptor-associated factor 1 was higher in the oral squamous cell carcinoma group as compared with the expression in the oral mucosa (p oral dysplasia (p oral squamous cell carcinoma. The patient survival rate was lower in the highly expressed tumor necrosis factor receptor-associated factor 1 group, although the difference was not significant. The clustering analysis showed that tumor necrosis factor receptor-associated factor 1 was most related to aldehyde dehydrogenase 1. These findings suggest that tumor necrosis factor receptor-associated factor 1 has potential direct/indirect regulations with the cancer stem cell markers in oral squamous cell carcinoma, which may help in further analysis of the cancer stem cell characteristics.

An Extension of the Lin-Kernighan-Helsgaun TSP Solver for Constrained Traveling Salesman and Vehicle Routing Problems

DEFF Research Database (Denmark)

Helsgaun, Keld

This report describes the implementation of an extension of the Lin-Kernighan-Helsgaun TSP solver for solving constrained traveling salesman and vehicle routing problems. The extension, which is called LKH-3, is able to solve a variety of well-known problems, including the sequential ordering...... problem (SOP), the traveling repairman problem (TRP), variants of the multiple travel-ing salesman problem (mTSP), as well as vehicle routing problems (VRPs) with capacity, time windows, pickup-and-delivery and distance constraints. The implementation of LKH-3 builds on the idea of transforming...... the problems into standard symmetric traveling salesman problems and handling constraints by means of penalty functions. Extensive testing on benchmark instances from the literature has shown that LKH-3 is effective. Best known solutions are often obtained, and in some cases, new best solutions are found...

Formation of positive cluster ions Li(n) Br (n = 2-7) and ionization energies studied by thermal ionization mass spectrometry.

Science.gov (United States)

Veličković, S R; Đustebek, J B; Veljković, F M; Veljković, M V

2012-05-01

Clusters of the type Li(n)X (X = halides) can be considered as potential building blocks of cluster-assembly materials. In this work, Li(n)Br (n = 2-7) clusters were obtained by a thermal ionization source of modified design and selected by a magnetic sector mass spectrometer. Positive ions of the Li(n)Br (n = 4-7) cluster were detected for the first time. The order of ion intensities was Li(2)Br(+) > Li(4)Br(+) > Li(5)Br(+) > Li(6)Br(+) > Li(3)Br(+). The ionization energies (IEs) were measured and found to be 3.95 ± 0.20 eV for Li(2)Br, 3.92 ± 0.20 eV for Li(3)Br, 3.93 ± 0.20 eV for Li(4)Br, 4.08 ± 0.20 eV for Li(5)Br, 4.14 ± 0.20 eV for Li(6)Br and 4.19 ± 0.20 eV for Li(7)Br. All of these clusters have a much lower ionization potential than that of the lithium atom, so they belong to the superalkali class. The IEs of Li(n)Br (n = 2-4) are slightly lower than those in the corresponding small Li(n) or Li(n)H clusters, whereas the IEs of Li(n)Br are very similar to those of Li(n) or Li(n)H for n = 5 and 6. The thermal ionization source of modified design is an important means for simultaneously obtaining and measuring the IEs of Li(n)Br (n = 2-7) clusters (because their ions are hermodynamically stable with respect to the loss of lithium atoms in the gas phase) and increasingly contributes toward the development of clusters for practical applications. Copyright © 2012 John Wiley & Sons, Ltd.

Lin's theory of flux and nuclear reactions

International Nuclear Information System (INIS)

Ping-Wha Lin

2002-01-01

Mathematical development of Lin's theory of flux is presented. Based on the Theory, when a chemical reaction system is subjected to a high time rate of temperature change, it changes from equilibrium to non-equilibrium conditions. It is proved mathematically that, when a gas system is subjected to a high time rate of temperature increase, the activities of particles (molecules, atoms or nuclei, and electrons) are increased: the particles are accelerated; frequencies and amplitudes of electron and atomic vibrations in a molecule increased; average kinetic energy of the particles increased; atomic bonds are ruptured; electrons are caused to leave their orbits. If most or all of the electrons leave their orbits, the gas fluid becomes plasma, which is very active chemically. The acceleration of nuclei in the dynamic condition can lead to nuclear reactions. In the pilot plant studies conducted at Research Triangle, NC, USA, for SO 2 conversion to SO 3 by rapid heating, a 10-ft high vertically fired combustor (VFC) was used. Air containing 0.5% SO 2 is forced continuously through the VFC, where it is heated by burners for conversion of SO 2 to SO 3 . During the idle period of operation, no external heat is added to the system by turning off the burners. It is observed that, as the air passing through the VFC during the idle period of sixteen hours, the temperature of the flowing air consistently rises up rapidly from ambient temperature (90 deg F) at inlet of the VFC to an average temperature as high as 582 deg F (in the range of 840 deg F to 455 deg F) at one section of the VFC, an increase of about 500 deg F. The air flow temperature increase of such large magnitude and long duration clearly indicates that nuclear reactions are present in VFC. It is also found that the water vapour in the air stream has completely disappeared in the VFC, for no sulphuric acid formation resulting from the reaction of water and SO 3 is detected there. Presumably, the water vapour in the

Functional comparison of the nematode Hox gene lin-39 in C. elegans and P. pacificus reveals evolutionary conservation of protein function despite divergence of primary sequences

OpenAIRE

Grandien, Kaj; Sommer, Ralf J.

2001-01-01

Hox transcription factors have been implicated in playing a central role in the evolution of animal morphology. Many studies indicate the evolutionary importance of regulatory changes in Hox genes, but little is known about the role of functional changes in Hox proteins. In the nematodes Pristionchus pacificus and Caenorhabditis elegans, developmental processes can be compared at the cellular, genetic, and molecular levels and differences in gene function can be identified. The Hox gene lin-3...

Digital cultural heritage and its applications: case studies of Digital Mogao Grottos and Chi Lin Nunnery reconstruction

Science.gov (United States)

Li, Deren; Du, Zhiqiang; Zhu, Yixuan; Wang, Tingsong

2010-11-01

Considerable damage has been done to the cultural heritage sites around the world ranging from natural erosion to artificial destruction. With the development of information sciences, frontier technologies are actively introduced to help protect cultural heritage sites. The new concept of a Digital Cultural Heritage has been presented for culture protection and is gradually becoming an efficient method to solve or to remit various difficult problems. This paper puts forward a digitalization method for cultural heritage sites which rationally integrates and utilizes multiform surveying measurements. These techniques have been successfully implemented into two projects, namely the Digital Mogao Grottos and the Chi Lin Nunnery reconstruction. Our results prove that the concept of and the techniques utilized in Digital Cultural Heritage can not only contribute to research, preservation, management, interpretation, and representation of cultural heritages but can also help resolve the conflicts between tourism and protection.

A Formalism for the Consistent Description of Non-Linear Elasticity of Anisotropic Media Formalisme pour une description cohérente de l'élasticité non linéaire des milieux anisotropes

Directory of Open Access Journals (Sweden)

Helbig K.

2006-12-01

deviation can be expressed by the relative change of the eigenstiffnesses and by the variation in the direction of the eigenstrains (expressed as vectors in six-dimensional strain space. The suggested procedure is applied to the two materials discussed by Rasolofosaon and Yin (1996. The results allow a heuristic evaluation of the meaning of the reference strain , the square root of the ratio of the norms of the fourth-rank and sixth-rank stiffness tensors. It is stressed that this is not a new theory of non-linearity, but only a different way of viewing the existing theory and results. La propagation des ondes élastiques est généralement traitée sous quatre hypothèses : - le milieu est isotrope, - le milieu est homogène, - il y a une relation biunivoque entre la tension et la déformation, - les tensions sont reliées d'une façon linéaire aux déformations (et de manière équivalente, les déformations sont reliées d'une façon linéaire aux tensions. En général au moins une de ces hypothèses - et souvent toutes - n'est pas vérifiée dans les milieux réels. Une description théorique valide de la propagation des ondes dans les milieux réels dépend ainsi de la description à la fois qualitative et quantitative de l'hétérogénéité, de l'anisotropie et de la non-linéarité : soit on doit supposer (ou montrer que l'écart par rapport à l'hypothèse de départ peut être - pour le problème considéré - négligé, soit on doit développer une description théorique, valide même en présence de ces écarts. Alors que l'effet d'un seul écart par rapport à un état idéal est relativement bien connu, les difficultés surviennent quand on veut combiner plusieurs de ces écarts. Les propriétés élastiques non linéaires d'échantillons de roche anisotropes (tricliniques ont été étudiées, par P. Rasolofosaon et H. Yin au 6e IWSA à Trondheim (Rasolofosaon et Yin, 1996. L'élasticité anisotrope non linéaire est importante seulement pour les amplitudes

A substrate specificity-determining unit of three Lin12-Notch repeat modules is formed in trans within the pappalysin-1 dimer and requires a sequence stretch C-terminal to the third module

DEFF Research Database (Denmark)

Weyer, Kathrin; Boldt, Henning B; Poulsen, Christine B

2007-01-01

-A cleaves IGFBP-4 and IGFBP-5, whereas PAPP-A2 cleaves only IGFBP-5. The pappalysins contain three Lin12-Notch repeat (LNR1-3) modules, previously considered unique to the Notch receptor family in which they function to regulate receptor cleavage. In contrast to the Notch receptor where three LNR modules...

Caractérisation microstructurale et mécanique d’un composite cimentaire renforcé par des fibres de lin Characterizations mechanical and microstructural of flax fibre cement composite reinforced

Directory of Open Access Journals (Sweden)

Boutouil M.

2012-09-01

Full Text Available Dans la perspective de valorisation des fibres de lin dans les matériaux de construction, la présente étude s’intéresse à la caractérisation microstructurale et mécanique d’un composite cimentaire renforcé par des fibres de lin. Les analyses microstructurales au MEB ont été menées pour évaluer l’homogénéité de la distribution des fibres, la qualité de l’interaction fibre/matrice et l’influence de leur présence sur les défauts microstructuraux. Le comportement mécanique en flexion du mortier renforcé par les fibres de lin est étudié en fonction de la longueur et la teneur en fibres. Les résultats indiquent une bonne adhésion entre les fibres et la matrice à l’état frais. Mais les fibres étant hydrophiles, elles gonflent pendant la prise du ciment et le retrait lors du séchage engendre alors des déchaussements. Les résultats de caractérisation mécanique sont encourageants. Tout d’abord, la fissuration du mortier due au retrait au jeune âge est fortement réduite du fait de la présence des fibres. Ensuite, la rupture brutale de la matrice en monolithe laisse place à un comportement quasi-ductile quand la teneur ou la longueur de fibre augmente. Ce changement de comportement, analysé en termes d’indice de ténacité, illustre la capacité remarquable des fibres de lin à renforcer les matrices cimentaires du fait de leurs bonnes propriétés mécaniques. With the purpose of the flax fibre valuing in construction materials, this study focuses on the characterizations mechanical and microstructural of flax fibre cement composite reinforced. The mechanical strength was studied as the function of fibre volume ratio and length. Meanwhile, the microstructural analysis investigated the homogeneity of fibre scattering, the interaction fibre/cement matrix and the influence of flax fibres on the defects microstructures. The results show the interesting mechanical properties of flax fibre in comparison with

In vitro and in vivo antimicrobial activities of seeds of Caesalpinia bonduc (Lin.) Roxb.

Science.gov (United States)

Arif, Tasleem; Mandal, T K; Kumar, Naresh; Bhosale, J D; Hole, Archana; Sharma, G L; Padhi, M M; Lavekar, G S; Dabur, Rajesh

2009-05-04

Caesalpinia bonduc (Lin.) Roxb. is a known drug in Ayurveda to treat various diseases specifically tumors, cysts and cystic fibrosis (CF). The aim of this study was to assess in vitro as well as in vivo antimicrobial activity of Caesalpinia bonduc seeds. The in vitro antimicrobial activities of seed coat and seed kernel extracts were investigated by microbroth dilution assay. In vivo activities of hydro-alcoholic extracts were investigated in rat models of chronic Pseudomonas aeruginosa pneumonia mimicking that in patients with cystic fibrosis. Various extracts of plant seeds exhibited in vitro antimicrobial activities in a range of 22-350 microg/ml. The extracts also showed activity against methicillin resistant (MR) Staphylococcus aureus and ampicillin resistant (AR) Pseudomonas aeruginosa as in the sensitive strains. In rat model of chronic Pseudomonas aeruginosa pneumonia, hydro-alcoholic extracts of Caesalpinia bonduc seed kernel (CBSK) and Caesalpinia bonduc seed coat (CBSC) were injected subcutaneously in the test groups of animals. The control groups were treated with cortisone and saline. Two weeks after challenge with Pseudomonas aeruginosa, the CBSK treated animals showed a significant bacterial clearance from the lungs (PCaesalpinia bonduc may have the potential to be promising natural medicine, with other forms of treatments, for CF patients with chronic Pseudomonas aeruginosa lung infections.

Les vélins du Muséum national d’histoire naturelle : une collection mise en lumière

OpenAIRE

Cardinal, Catherine

2018-01-01

Une exposition unique au Cabinet d’histoire du Jardin des plantes Très rarement, le public a pu voir la collection d’images de fleurs, de plantes, d’oiseaux et de mammifères, déposée à la bibliothèque centrale du Musée national d’histoire naturelle. La fragilité des peintures sur vélin au contact de l’air humide et de la lumière explique les précautions prises à leur égard. L’exposition d’environ cent cinquante pièces au sein du Cabinet d’histoire, d’octobre 2016 à janvier 2017, a donc consti...

É tempo de pipa: a representação da infância em Cidade de Deus, de Paulo Lins, e Lembrancinha do Adeus, de Júlio Ludemir

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Anderson Luís Nunes da Mata

2009-01-01

Full Text Available Traditionally understood as an age of innocence and learning, childhood has been shown indifferent colors in the so called "slum" narratives. Thus, poor children, who live in the urban peripheral areas, are represented in fiction as a counterface for such traditional notion of childhood. Lembrancinha do Adeus, by Julio Ludemir, and Cidade de Deus, by Paulo Lins, are both novels built upon characters who maintain only remains of childhood, which indicates both a new sociability for such children and deeper social changes.

Multi-Ferroic Polymer Nanoparticle Composites for Next Generation Metamaterials

Science.gov (United States)

2016-06-15

These conditions (increases the alkalanity of the reaction media and presence of hydroxides ) are well known to favor the co-precipitation of iron...oxide NGs from intermediate iron hydroxides . That would explain the observation of small RSNs after 5 h of reaction. RSN PLS PLS decompositionGel...or gold in collaboration with Dr Yuanzhe Piao. Remarks : Prof Peter Kofinas has performed courses at Strasbourg (12h eq TD) as invited professor

Gene expression profile and functionality of ESC-derived Lin-ckit+Sca-1+ cells are distinct from Lin-ckit+Sca-1+ cells isolated from fetal liver or bone marrow.

Directory of Open Access Journals (Sweden)

Irina Fernandez

Full Text Available In vitro bioreactor-based cultures are being extensively investigated for large-scale production of differentiated cells from embryonic stem cells (ESCs. However, it is unclear whether in vitro ESC-derived progenitors have similar gene expression profiles and functionalities as their in vivo counterparts. This is crucial in establishing the validity of ESC-derived cells as replacements for adult-isolated cells for clinical therapies. In this study, we compared the gene expression profiles of Lin-ckit+Sca-1+ (LKS cells generated in vitro from mouse ESCs using either static or bioreactor-based cultures, with that of native LKS cells isolated from mouse fetal liver (FL or bone marrow (BM. We found that in vitro-generated LKS cells were more similar to FL- than to BM LKS cells in gene expression. Further, when compared to cells derived from bioreactor cultures, static culture-derived LKS cells showed fewer differentially expressed genes relative to both in vivo LKS populations. Overall, the expression of hematopoietic genes was lower in ESC-derived LKS cells compared to cells from BM and FL, while the levels of non-hematopoietic genes were up-regulated. In order to determine if these molecular profiles correlated with functionality, we evaluated ESC-derived LKS cells for in vitro hematopoietic-differentiation and colony formation (CFU assay. Although static culture-generated cells failed to form any colonies, they did differentiate into CD11c+ and B220+ cells indicating some hematopoietic potential. In contrast, bioreactor-derived LKS cells, when differentiated under the same conditions failed to produce any B220+ or CD11c+ cells and did not form colonies, indicating that these cells are not hematopoietic progenitors. We conclude that in vitro culture conditions significantly affect the transcriptome and functionality of ESC-derived LKS cells and although in vitro differentiated LKS cells were lineage negative and expressed both ckit and Sca-1

Stepwise dissection and visualization of the catalytic mechanism of haloalkane dehalogenase LinB using molecular dynamics simulations and computer graphics.

Science.gov (United States)

Negri, Ana; Marco, Esther; Damborsky, Jiri; Gago, Federico

2007-10-01

The different steps of the dehalogenation reaction carried out by LinB on three different substrates have been characterized using a combination of quantum mechanical calculations and molecular dynamics simulations. This has allowed us to obtain information in atomic detail about each step of the reaction mechanism, that is, substrate entrance and achievement of the near-attack conformation, transition state stabilization within the active site, halide stabilization, water molecule activation and subsequent hydrolytic attack on the ester intermediate with formation of alcohol, and finally product release. Importantly, no bias or external forces were applied during the whole procedure so that both intermediates and products were completely free to sample configuration space in order to adapt to the plasticity of the active site and/or search for an exit. Differences in substrate reactivity were found to be correlated with the ease of adopting the near-attack conformation and two different exit pathways were found for product release that do not interfere with substrate entrance. Additional support for the different entry and exit pathways was independently obtained from an examination of the enzyme's normal modes.

Etude de la stabilité globale de l’équilibre endémique des modèles multi groupes SIR avec une incidence non linéaire.

OpenAIRE

BENCHAIB, NESRINE

2014-01-01

Dans ce mémoire, on présente le nombre de reproduction de base pour un modèle épidémique multigroupe avec une incidence non linéaire. Ensuite, on établie la dynamique globale est entièrement déterminée par le nombre de reproduction de base R0. On montre que le nombre de reproduction de base R0 est un paramètre global de seuil dans le sens que si il est inférieur ou égale à 1, l’équilibre sans maladie est globalement stable et la maladie s’éteint, alors que si il est supérieur à 1, il est u...

Source apportionment of PM2.5 at the Lin'an regional background site in China with three receptor models

Science.gov (United States)

Deng, Junjun; Zhang, Yanru; Qiu, Yuqing; Zhang, Hongliang; Du, Wenjiao; Xu, Lingling; Hong, Youwei; Chen, Yanting; Chen, Jinsheng

2018-04-01

Source apportionment of fine particulate matter (PM2.5) were conducted at the Lin'an Regional Atmospheric Background Station (LA) in the Yangtze River Delta (YRD) region in China from July 2014 to April 2015 with three receptor models including principal component analysis combining multiple linear regression (PCA-MLR), UNMIX and Positive Matrix Factorization (PMF). The model performance, source identification and source contribution of the three models were analyzed and inter-compared. Source apportionment of PM2.5 was also conducted with the receptor models. Good correlations between the reconstructed and measured concentrations of PM2.5 and its major chemical species were obtained for all models. PMF resolved almost all masses of PM2.5, while PCA-MLR and UNMIX explained about 80%. Five, four and seven sources were identified by PCA-MLR, UNMIX and PMF, respectively. Combustion, secondary source, marine source, dust and industrial activities were identified by all the three receptor models. Combustion source and secondary source were the major sources, and totally contributed over 60% to PM2.5. The PMF model had a better performance on separating the different combustion sources. These findings improve the understanding of PM2.5 sources in background region.

Enhanced immunization via dissolving microneedle array-based delivery system incorporating subunit vaccine and saponin adjuvant

OpenAIRE

Zhao,JiHui; Zhang,Qi-Bo; Liu,Bao; Piao,Xiang-Hua; Yan,Yu-Lu; Hu,Xiao-Ge; Zhou,Kuan; Zhang,Yong-Tai; Feng,Nian-Ping

2017-01-01

Ji-Hui Zhao,1,* Qi-Bo Zhang,1,* Bao Liu,2 Xiang-Hua Piao,1 Yu-Lu Yan,1 Xiao-Ge Hu,1 Kuan Zhou,1 Yong-Tai Zhang,1 Nian-Ping Feng1 1School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 2Anethesiology Department, Augusta University, Augusta, GA, USA *These authors contributed equally to this work Purpose: To enhance the immunogenicity of the model subunit vaccine, ovalbumin (OVA) was combined with platycodin (PD), a ...

Evaluación preclínica y estudio de estabilidad de extractos a partir del follaje de Momordica charantia Lin

Directory of Open Access Journals (Sweden)

A. Lagarto

2014-12-01

Full Text Available El objetivo del presente trabajo fue la evaluación preclínica y el estudio de estabilidad de extractos a partir del follaje de Momordica charantia Lin. Se obtuvieron extractos acuoso e hidroalcohólico para los cuales se establecieron las especificaciones de calidad mediante la evaluación de tres lotes y se estudió su estabilidad por el método de vida de estante durante 12 meses. A los extractos se le evaluó el potencial genotóxico mediante ensayos de micronúcleos en médula ósea de ratón y aberraciones cromosómicas en linfocitos de sangre periférica. La actividad hipoglicemiante oral fue evaluada en animales con hiperglicemia temporal inducida por carga de glucosa. Como resultados se establecieron las especificaciones de calidad de los extractos acuoso e hidroalcohólico, los mismos mostraron estabilidad por 6 meses para el extracto acuoso y 12 meses para el extracto hidroalcohólico. No mostraron efecto genotóxico en los ensayos evaluados y mostraron efecto hipoglicemiante oral a la dosis de 450 mg/kg.

A Real-Time Implementable NMPC Output Feedback for a Diesel Engine Air Path Commande prédictive non linéaire avec retour de sortie implémentable en temps-réel pour un circuit d’air d’un moteur Diesel

Directory of Open Access Journals (Sweden)

Murilo A.

2011-09-01

Full Text Available In this paper, an experimental validation of a parameterized Nonlinear Model Predictive Control (NMPC scheme with a Moving Horizon Observer (MHO is presented for a Diesel engine air path. The control design based on the parameterized approach leads to a low dimensional optimization problem which makes the proposed controller real-time implementable. An attractive feature of this control strategy lies in its compatibility with more elaborated and fully nonlinear models since it uses the model as a black box predictor. Dans cet article, une validation expérimentale d’un schéma de commande prédictive non linéaire (CPNL paramétrique avec un observateur à horizon glissant a été proposé pour le circuit d’air d’un moteur Diesel. La stratégie de commande basée sur l’approche paramétrique à faible dimension s’est montrée implémentable en temps réel et peut être utilisée aussi en tant qu’une solution du type boîte noire indépendamment de la structure du modèle non linéaire.

Constrained Versions of DEDICOM for Use in Unsupervised Part-Of-Speech Tagging

Energy Technology Data Exchange (ETDEWEB)

Dunlavy, Daniel; Peter A. Chew

2016-05-01

This reports describes extensions of DEDICOM (DEcomposition into DIrectional COMponents) data models [3] that incorporate bound and linear constraints. The main purpose of these extensions is to investigate the use of improved data models for unsupervised part-of-speech tagging, as described by Chew et al. [2]. In that work, a single domain, two-way DEDICOM model was computed on a matrix of bigram fre- quencies of tokens in a corpus and used to identify parts-of-speech as an unsupervised approach to that problem. An open problem identi ed in that work was the com- putation of a DEDICOM model that more closely resembled the matrices used in a Hidden Markov Model (HMM), speci cally through post-processing of the DEDICOM factor matrices. The work reported here consists of the description of several models that aim to provide a direct solution to that problem and a way to t those models. The approach taken here is to incorporate the model requirements as bound and lin- ear constrains into the DEDICOM model directly and solve the data tting problem as a constrained optimization problem. This is in contrast to the typical approaches in the literature, where the DEDICOM model is t using unconstrained optimization approaches, and model requirements are satis ed as a post-processing step.

Stress Survival Islet 2, Predominantly Present in Listeria monocytogenes Strains of Sequence Type 121, Is Involved in the Alkaline and Oxidative Stress Responses.

Science.gov (United States)

Harter, Eva; Wagner, Eva Maria; Zaiser, Andreas; Halecker, Sabrina; Wagner, Martin; Rychli, Kathrin

2017-08-15

The foodborne pathogen Listeria monocytogenes is able to survive a variety of stress conditions leading to the colonization of different niches like the food processing environment. This study focuses on the hypervariable genetic hot spot lmo0443 to lmo0449 haboring three inserts: the stress survival islet 1 (SSI-1), the single-gene insert LMOf2365_0481 , and two homologous genes of the nonpathogenic species Listeria innocua : lin0464 , coding for a putative transcriptional regulator, and lin0465 , encoding an intracellular PfpI protease. Our prevalence study revealed a different distribution of the inserts between human and food-associated isolates. The lin0464-lin0465 insert was predominantly found in food-associated strains of sequence type 121 (ST121). Functional characterization of this insert showed that the putative PfpI protease Lin0465 is involved in alkaline and oxidative stress responses but not in acidic, gastric, heat, cold, osmotic, and antibiotic stresses. In parallel, deletion of lin0464 decreased survival under alkaline and oxidative stresses. The expression of both genes increased significantly under oxidative stress conditions independently of the alternative sigma factor σ B Furthermore, we showed that the expression of the protease gene lin0465 is regulated by the transcription factor lin0464 under stress conditions, suggesting that lin0464 and lin0465 form a functional unit. In conclusion, we identified a novel stress survival islet 2 (SSI-2), predominantly present in L. monocytogenes ST121 strains, beneficial for survival under alkaline and oxidative stresses, potentially supporting adaptation and persistence of L. monocytogenes in food processing environments. IMPORTANCE Listeria monocytogenes strains of ST121 are known to persist for months and even years in food processing environments, thereby increasing the risk of food contamination and listeriosis. However, the molecular mechanism underlying this remarkable niche-specific adaptation

Identification of key factors regulating self-renewal and differentiation in EML hematopoietic precursor cells by RNA-sequencing analysis.

Science.gov (United States)

Zong, Shan; Deng, Shuyun; Chen, Kenian; Wu, Jia Qian

2014-11-11

Hematopoietic stem cells (HSCs) are used clinically for transplantation treatment to rebuild a patient's hematopoietic system in many diseases such as leukemia and lymphoma. Elucidating the mechanisms controlling HSCs self-renewal and differentiation is important for application of HSCs for research and clinical uses. However, it is not possible to obtain large quantity of HSCs due to their inability to proliferate in vitro. To overcome this hurdle, we used a mouse bone marrow derived cell line, the EML (Erythroid, Myeloid, and Lymphocytic) cell line, as a model system for this study. RNA-sequencing (RNA-Seq) has been increasingly used to replace microarray for gene expression studies. We report here a detailed method of using RNA-Seq technology to investigate the potential key factors in regulation of EML cell self-renewal and differentiation. The protocol provided in this paper is divided into three parts. The first part explains how to culture EML cells and separate Lin-CD34+ and Lin-CD34- cells. The second part of the protocol offers detailed procedures for total RNA preparation and the subsequent library construction for high-throughput sequencing. The last part describes the method for RNA-Seq data analysis and explains how to use the data to identify differentially expressed transcription factors between Lin-CD34+ and Lin-CD34- cells. The most significantly differentially expressed transcription factors were identified to be the potential key regulators controlling EML cell self-renewal and differentiation. In the discussion section of this paper, we highlight the key steps for successful performance of this experiment. In summary, this paper offers a method of using RNA-Seq technology to identify potential regulators of self-renewal and differentiation in EML cells. The key factors identified are subjected to downstream functional analysis in vitro and in vivo.

Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction

Directory of Open Access Journals (Sweden)

Creer Michael H

2010-03-01

Full Text Available Abstract Human stem cells from adult sources have been shown to contribute to the regeneration of muscle, liver, heart, and vasculature. The mechanisms by which this is accomplished are, however, still not well understood. We tested the engraftment and regenerative potential of human umbilical cord blood-derived ALDHhiLin-, and ALDHloLin- cells following transplantation to NOD/SCID or NOD/SCID β2m null mice with experimentally induced acute myocardial infarction. We used combined nanoparticle labeling and whole organ fluorescent imaging to detect human cells in multiple organs 48 hours post transplantation. Engraftment and regenerative effects of cell treatment were assessed four weeks post transplantation. We found that ALDHhiLin- stem cells specifically located to the site of injury 48 hours post transplantation and engrafted the infarcted heart at higher frequencies than ALDHloLin- committed progenitor cells four weeks post transplantation. We found no donor derived cardiomyocytes and few endothelial cells of donor origin. Cell treatment was not associated with any detectable functional improvement at the four week endpoint. There was, however, a significant increase in vascular density in the central infarct zone of ALDHhiLin- cell-treated mice, as compared to PBS and ALDHloLin- cell-treated mice. Conclusions Our data indicate that adult human stem cells do not become a significant part of the regenerating tissue, but rapidly home to and persist only temporarily at the site of hypoxic injury to exert trophic effects on tissue repair thereby enhancing vascular recovery.

Nonlinear EGR and VGT Control with Integral Action for Diesel Engines Régulation de Recirculation des Gaz d’Echappement (RGE et de Turbine à Géométrie Variable (TGV non linéaire avec action intégrée pour moteurs Diesel

Directory of Open Access Journals (Sweden)

Wahlström J.

2011-10-01

Full Text Available A nonlinear multivariable control design with integral action is proposed and investigated for control of Exhaust Gas Recirculation (EGR and Variable Geometry Turbine (VGT in heavy duty Diesel engines. The main control goal is to regulate oxygen/fuel ratio and intake manifold EGR-fraction, and they are specified in an outer loop. These are chosen as main performance variables since they are strongly coupled to the emissions. An existing nonlinear control design based on feedback linearization is extended with integral action. In particular, the control design method utilizes a control Lyapunov function, inverse optimal control, and a nonlinear input transformation. Comparisons between different control structures are performed in simulations showing the following four points. Firstly, integral action is necessary to handle model errors so that the controller can track the performance variables specified in the outer loop. Secondly, the proposed control design handles the nonlinear effects in the Diesel engine that results in less control errors compared to a control structure with PID controllers. Thirdly, it is important to use the input transformation and it is sufficient to use a control structure with PID controllers and input transformation to handle the nonlinear effects. Fourthly, the proposed control design is sensitive to model errors in the input transformation while a control structure with PID controllers and input transformation handles these model errors. Une conception de régulation non linéaire à variables multiples avec action intégrée est proposée et étudiée pour une régulation de la Recirculation des Gaz d’Echappement (RGE et une Turbine à Géométrie Variable (TGV au sein de moteurs Diesel à usage industriel. L’objectif principal de la régulation consiste à réguler le rapport oxygène / carburant et la fraction de RGE de tubulure d’admission, qui sont spécifiés au sein d’une boucle extérieure. Ceux

Iodine Deficiency in a Study Population of Norwegian Pregnant Women-Results from the Little in Norway Study (LiN).

Science.gov (United States)

Dahl, Lisbeth; Wik Markhus, Maria; Sanchez, Perla Vanessa Roldan; Moe, Vibeke; Smith, Lars; Meltzer, Helle Margrete; Kjellevold, Marian

2018-04-20

Iodine sufficiency is particularly important in pregnancy, where median urinary iodine concentration (UIC) in the range of 150⁻250 µg/L indicates adequate iodine status. The aims of this study were to determine UIC and assess if dietary and maternal characteristics influence the iodine status in pregnant Norwegian women. The study comprises a cross-sectional population-based prospective cohort of pregnant women (Little in Norway (LiN)). Median UIC in 954 urine samples was 85 µg/L and 78.4% of the samples ( n = 748) were ≤150 µg/L. 23.2% ( n = 221) of the samples were ≤50 µg/L and 5.2% ( n = 50) were above the requirements of iodine intake (>250 µg/L). Frequent iodine-supplement users ( n = 144) had significantly higher UIC (120 µg/L) than non-frequent users (75 µg/L). Frequent milk and dairy product consumers (4⁻9 portions/day) had significantly higher UIC (99 µg/L) than women consuming 0⁻1 portion/day (57 µg/L) or 2⁻3 portions/day (83 µg/L). Women living in mid-Norway ( n = 255) had lowest UIC (72 µg/L). In conclusion, this study shows that the diet of the pregnant women did not necessarily secure a sufficient iodine intake. There is an urgent need for public health strategies to secure adequate iodine nutrition among pregnant women in Norway.

Linear and Non-Linear Piezoresistance Coefficients in Cubic Semiconductors. I. Theoretical Formulations

Science.gov (United States)

Durand, S.; Tellier, C. R.

1996-02-01

This paper constitutes the first part of a work devoted to applications of piezoresistance effects in germanium and silicon semiconductors. In this part, emphasis is placed on a formal explanation of non-linear effects. We propose a brief phenomenological description based on the multi-valleys model of semiconductors before to adopt a macroscopic tensorial model from which general analytical expressions for primed non-linear piezoresistance coefficients are derived. Graphical representations of linear and non-linear piezoresistance coefficients allows us to characterize the influence of the two angles of cut and of directions of alignment. The second part will primarily deal with specific applications for piezoresistive sensors. Cette publication constitue la première partie d'un travail consacré aux applications des effets piézorésistifs dans les semiconducteurs germanium et silicium. Cette partie traite essentiellement de la modélisation des effets non-linéaires. Après une description phénoménologique à partir du modèle de bande des semiconducteurs nous développons un modèle tensoriel macroscopique et nous proposons des équations générales analytiques exprimant les coefficients piézorésistifs non-linéaires dans des repères tournés. Des représentations graphiques des variations des coefficients piézorésistifs linéaires et non-linéaires permettent une pré-caractérisation de l'influence des angles de coupes et des directions d'alignement avant l'étude d'applications spécifiques qui feront l'objet de la deuxième partie.

Lithology identification of aquifers from geophysical well logs and fuzzy logic analysis: Shui-Lin Area, Taiwan

Science.gov (United States)

Hsieh, Bieng-Zih; Lewis, Charles; Lin, Zsay-Shing

2005-04-01

The purpose of this study is to construct a fuzzy lithology system from well logs to identify formation lithology of a groundwater aquifer system in order to better apply conventional well logging interpretation in hydro-geologic studies because well log responses of aquifers are sometimes different from those of conventional oil and gas reservoirs. The input variables for this system are the gamma-ray log reading, the separation between the spherically focused resistivity and the deep very-enhanced resistivity curves, and the borehole compensated sonic log reading. The output variable is groundwater formation lithology. All linguistic variables are based on five linguistic terms with a trapezoidal membership function. In this study, 50 data sets are clustered into 40 training sets and 10 testing sets for constructing the fuzzy lithology system and validating the ability of system prediction, respectively. The rule-based database containing 12 fuzzy lithology rules is developed from the training data sets, and the rule strength is weighted. A Madani inference system and the bisector of area defuzzification method are used for fuzzy inference and defuzzification. The success of training performance and the prediction ability were both 90%, with the calculated correlation of training and testing equal to 0.925 and 0.928, respectively. Well logs and core data from a clastic aquifer (depths 100-198 m) in the Shui-Lin area of west-central Taiwan are used for testing the system's construction. Comparison of results from core analysis, well logging and the fuzzy lithology system indicates that even though the well logging method can easily define a permeable sand formation, distinguishing between silts and sands and determining grain size variation in sands is more subjective. These shortcomings can be improved by a fuzzy lithology system that is able to yield more objective decisions than some conventional methods of log interpretation.

Heterodimerization with the β1 subunit directs the α2 subunit of nitric oxide-sensitive guanylyl cyclase to calcium-insensitive cell-cell contacts in HEK293 cells: Interaction with Lin7a.

Science.gov (United States)

Hochheiser, Julia; Haase, Tobias; Busker, Mareike; Sömmer, Anne; Kreienkamp, Hans-Jürgen; Behrends, Sönke

2016-12-15

Nitric oxide-sensitive guanylyl cyclase is a heterodimeric enzyme consisting of an α and a β subunit. Two different α subunits (α 1 and α 2 ) give rise to two heterodimeric enzymes α 1 /β 1 and α 2 /β 1 . Both coexist in a wide range of tissues including blood vessels and the lung, but expression of the α 2 /β 1 form is generally much lower and approaches levels similar to the α 1 /β 1 form in the brain only. In the present paper, we show that the α 2 /β 1 form interacts with Lin7a in mouse brain synaptosomes based on co-precipitation analysis. In HEK293 cells, we found that the overexpressed α 2 /β 1 form, but not the α 1 /β 1 form is directed to calcium-insensitive cell-cell contacts. The isolated PDZ binding motif of an amino-terminally truncated α 2 subunit was sufficient for cell-cell contact localization. For the full length α 2 subunit with the PDZ binding motif this was only the case in the heterodimer configuration with the β 1 subunit, but not as isolated α 2 subunit. We conclude that the PDZ binding motif of the α 2 subunit is only accessible in the heterodimer conformation of the mature nitric oxide-sensitive enzyme. Interaction with Lin7a, a small scaffold protein important for synaptic function and cell polarity, can direct this complex to nectin based cell-cell contacts via MPP3 in HEK293 cells. We conclude that heterodimerization is a prerequisite for further protein-protein interactions that direct the α 2 /β 1 form to strategic sites of the cell membrane with adjacent neighbouring cells. Drugs increasing the nitric oxide-sensitivity of this specific form may be particularly effective. Copyright © 2016 Elsevier Inc. All rights reserved.

Effects of gamma radiation on various stages in the life cycle of meal worm, Tenebrio molitor Lin

International Nuclear Information System (INIS)

Chiravathanapong, S.; Segsarnviriya, S.

1984-12-01

Effect of gamma radiation on each growth stage of meal worm (Tenebrio molitor Lin.) were conducted at 27+-2 0 C and 75+-2% relative humidity. LD 50 and LD 99 of 5-day old eggs at 5 days after irradiation were 76 and 367 gray. The survived larvae could live no longer than 5 days and seldom moved or without feeding. LD 50 and LD 99 of last instar larvae at 10 days after irradiation were 662 and 1,367 gray. No pupation occurred in larvae after irradiation. Dead larvae caused by radiation turned blackish, liquid oozed out from the body, and survived no longer than 5 days. Some irradiated larvae had incomplete molting and died. LD 50 and LD 99 of 6-day-old pupae at 3 days after irradiation were 874 and 1,492 gray. Abnormal irradiated pupae, some abnormal pupae were underdeveloped wing and abdominal pupal characteristic was still remained. Pupal legs could develop to adult legs only and could survive no longer than 1 week. Unfortunately pupae irradiated at low dosage of radiation would develop to be complete adult head, but could survive no longer than 2 weeks with slow movement and no oviposition. LD 50 of male adults and female adults was checked in 7 days postirradiation and it was estimated as 788 and 786 gray while LD 99 was estimated as 1,375 and 1,350 gray respectively. Results of male adults and female adults checking in 10 days post-irradiation found that LD 50 was estimated as 533 and 598 gray and LD 99 was estimated as 1,227 and 1,229 gray respectively. The remained survivors of both sexes could survive no longer than 10 days without feeding and mating

LIN Dataset

Data.gov (United States)

Department of Homeland Security — This data is captured and helps to coordinate technical and scientific testing in the area of Trade Enforcement, WMD, Intellectual Property Rights, and Narcotics...

A Hydrological Tomography Collocated with Time-varying Gravimetry for Hydrogeology -An Example in Yun-Lin Alluvial Plain and Ming-Ju Basin in Taiwan

Science.gov (United States)

Chen, K. H.; Cheng, C. C.; Hwang, C.

2016-12-01

A new inversion technique featured by the collocation of hydrological modeling and gravimetry observation is presented in this report. Initially this study started from a project attempting to build a sequence of hydrodynamic models of ground water system, which was applied to identify the supplement areas of alluvial plains and basins along the west coast of Taiwan. To calibrate the decent hydro-geological parameters for the modeling, geological evolution were carefully investigated and absolute gravity observations, along with other on-site hydrological monitoring data were specially introduced. It was discovered in the data processing that the time-varying gravimetrical data are highly sensitive to certain boundary conditions in the hydrodynamic model, which are correspondent with respective geological features. A new inversion technique coined by the term "hydrological tomography" is therefore developed by reversing the boundary condition into the unknowns to be solved. An example of accurate estimate for water storage and precipitation infiltration of a costal alluvial plain Yun-Lin is presented. In the mean time, the study of an anticline structure of the upstream basin Ming-Ju is also presented to demonstrate how a geological formation is outlined when the gravimetrical data and hydrodynamic model are re-directed into an inversion.

Uso agrícola de esgoto tratado em lagoas de estabilização:experiências do Pprosab* em Lins – SP

Directory of Open Access Journals (Sweden)

Fernando Mendonça Campos

2004-01-01

Full Text Available A Pilot Plant to treat the effluent from SABESP´s Wastewater Treatment Plant (Stabilization Pond System located at Lins was built within a six-hectare area adjacent to the Treatment Plant. The study focused on agricultural applications of the final treated effluent. Additional unit operations for wastewater treatment were constructed – namely hypochlorite disinfection, UV light disinfection and ozonation units. The final effluent from the Pilot Plant was applied in crop irrigation – namely coffee, corn and bean crops and the feeding system was by drop irrigation. Additionally, a fraction of the final treated effluent fed a hydrophonic system installed in vases, in which chrysanthemum and Gypsophila were cultivated. A third feeding line supplied a fish tank in which Oreochromis Niloticus were grown. A number of physical, chemical and biological variables were utilized for quality control of effluent streams at the various treatment stages, to monitor the cultivated crops and the soil, as well as agricultural parameters to identify growth characteristics. For all the cases under study there were conventional mode crops in parallel, acting as controls or references. The results obtained showed that the pond effluent, besides the hydraulic capacity itself, contained nutrients that are adequate for agricultural purposes, with significant savings associated. Additionally, such applications in irrigation avoid the eutrophication of natural water bodies

Vers l’actualisation d’un « récit fantôme » ?

OpenAIRE

Campion, Baptiste

2009-01-01

Parmi les caractéristiques des « nouveaux » médias (sous-entendu : numériques) les plus souvent mises en avant, on retient généralement la non-linéarité, la réticularité et l’interactivité. Elles permettent de catégoriser les supports numériques à part des grands médias traditionnels, linéaires et non directement (par nature) réticulaires et interactifs. Pour ces raisons, les médias électroniques ont assurément ouvert des possibilités expressives nouvelles, dont les créateurs de tous types ne...

A liquefied energy chain for transport and utilization of natural gas for power production with CO2 capture and storage - Part 4: Sensitivity analysis of transport pressures and benchmarking with conventional technology for gas transport

International Nuclear Information System (INIS)

Aspelund, Audun; Gundersen, Truls

2009-01-01

A novel energy and cost effective transport chain for stranded natural gas utilized for power production with CO 2 capture and storage is developed. It includes an offshore section, a combined gas carrier and an integrated receiving terminal. In the offshore section, natural gas (NG) is liquefied to LNG by liquid carbon dioxide (LCO 2 ) and liquid inert nitrogen (LIN), which are used as cold carriers. In the onshore process, the cryogenic exergy in the LNG is utilized to cool and liquefy the cold carriers, LCO 2 and LIN. The transport pressures for LNG, LIN and LCO 2 will influence the thermodynamic efficiency as well as the ship utilization; hence sensitivity analyses are performed, showing that the ship utilization for the payload will vary between 58% and 80%, and the transport chain exergy efficiency between 48% and 52%. A thermodynamically optimized process requires 319 kWh/tonne LNG. The NG lost due to power generation needed to operate the LEC processes is roughly one third of the requirement in a conventional transport chain for stranded NG gas with CO 2 capture and sequestration (CCS)

Pleiotropic consequences of misexpression of the developmentally ...

Indian Academy of Sciences (India)

these conditions would affect activities of the hnRNPs and other hsrω-RNA interacting proteins, which is likely to have cascading ... junk', make up the major part of the genome output in higher ..... nervous system (Lin and Goodman 1994).

Antioxidant and free radical scavenging activities of plant extracts ...

African Journals Online (AJOL)

STORAGESEVER

2008-06-17

Jun 17, 2008 ... venging mechanism as part of their activity (Perry et al.,. 1999; Lin ... are used in traditional medicine (Argueta et al., 1994;. Monroy-Ortíz ..... Bermejo P, Iglesias I, Abad MJ, Gómez-Serranillos P, Liso PA, Villar. A, Chiriboga X ...

A Cross-Grade Study Validating the Evolutionary Pathway of Student Mental Models in Electric Circuits

Science.gov (United States)

Lin, Jing-Wen

2017-01-01

Cross-grade studies are valuable for the development of sequential curriculum. However such studies are time and resource intensive and fail to provide a clear representation to integrate different levels of representational complexity. Lin (Lin, 2006; Lin & Chiu, 2006; Lin, Chiu, & Hsu, 2006) proposed a cladistics approach in conceptual…

Relevance of the Lin's and Host hydropedological models to predict grape yield and wine quality

Directory of Open Access Journals (Sweden)

E. A. C. Costantini

2009-09-01

Full Text Available The adoption of precision agriculture in viticulture could be greatly enhanced by the diffusion of straightforward and easy to be applied hydropedological models, able to predict the spatial variability of available soil water. The Lin's and Host hydropedological models were applied to standard soil series descriptions and hillslope position, to predict the distribution of hydrological functional units in two vineyard and their relevance for grape yield and wine quality. A three-years trial was carried out in Chianti (Central Italy on Sangiovese. The soils of the vineyards differentiated in structure, porosity and related hydropedological characteristics, as well as in salinity. Soil spatial variability was deeply affected by earth movement carried out before vine plantation. Six plots were selected in the different hydrological functional units of the two vineyards, that is, at summit, backslope and footslope morphological positions, to monitor soil hydrology, grape production and wine quality. Plot selection was based upon a cluster analysis of local slope, topographic wetness index (TWI, and cumulative moisture up to the root limiting layer, appreciated by means of a detailed combined geophysical survey. Water content, redox processes and temperature were monitored, as well as yield, phenological phases, and chemical analysis of grapes. The isotopic ratio δ¹³C was measured in the wine ethanol upon harvesting to evaluate the degree of stress suffered by vines. The grapes in each plot were collected for wine making in small barrels. The wines obtained were analysed and submitted to a blind organoleptic testing.

The results demonstrated that the combined application of the two hydropedological models can be used for the prevision of the moisture status of soils cultivated with grape during summertime in Mediterranean climate. As correctly foreseen by the models, the amount of mean daily transpirable soil water (TSW during

Lin Simpson | NREL

Science.gov (United States)

technology areas that included: nanotechnology, nanomaterials, thin-film photovoltaics, thin-film processing , and program management. This includes extensive experience in nanotechnology, materials science B.S., Engineering Physics, Colorado School of Mines. Thesis: Spatial filtering for pattern recognition

ALLIUM HOOKERII LIN

African Journals Online (AJOL)

Toshiba

increase the food supply and add variety to the diet [5]. ... available in the Himalayan range including Nepal, China [6]. ... altitude, between 93.42oE to 94.47oE longitude and between 23.83 o ... The leaf decoction (Decoction is a method of extraction, by boiling, or ..... Dry fish washed in water are fried in a small amount of.

75 FR 76648 - Proposed Establishment of Area Navigation (RNAV) Routes; Western United States

Science.gov (United States)

2010-12-09

... routes would improve departure flow from the San Francisco/Oakland, CA, Terminal area by providing...'' N., long. 107[deg]55'30'' W.) * * * * * Q-128 LIN to MEM [New] LIN VORTAC (Lat. 38[deg]04'29'' N...'' W.) * * * * * Q-130 LIN to PNH [New] LIN VORTAC (Lat. 38[deg]04'29'' N., long. 121[deg]00'14'' W...

76 FR 13084 - Establishment of Area Navigation (RNAV) Routes; Western United States

Science.gov (United States)

2011-03-10

... Francisco/Oakland, CA, Terminal area by providing additional parallel departure routings and improve arrival....) * * * * * * * Q-128 LIN to MEM [New] LIN VORTAC (Lat. 38[deg]04[min]29[sec] N., long. 121[deg]00[min]14[sec] W...] W.) * * * * * * * Q-130 LIN to PNH [New] LIN VORTAC (Lat. 38[deg]04[min]29[sec] N., long. 121[deg]00...

Non-minimally coupled tachyonic inflation in warped string background

International Nuclear Information System (INIS)

Chingangbam, Pravabati; Panda, Sudhakar; Deshamukhya, Atri

2005-01-01

We show that the non-minimal coupling of tachyon field to the scalar curvature, as proposed by Piao et al, with the chosen coupling parameter does not produce the effective potential where the tachyon field can roll down from T=0 to large T along the slope of the potential. We find a correct choice of the parameters which ensures this requirement and support slow-roll inflation. However, we find that the cosmological parameter found from the analysis of the theory are not in the range obtained from observations. We then invoke warped compactification and varying dilaton field over the compact manifold, as proposed by Raeymaekers, to show that in such a setup the observed parameter space can be ensured. (author)

An Observational Study of a Prefrontal Convective Rainband Using Tamex Single-and Dual-Doppler Data

Science.gov (United States)

1991-01-01

integration from the surface. Other Doppler studies, e.g., Chong and Testud (1983), Lin et al. 37 (1986), etc, also showed similiar results. 4.3 Variational...Atmos. Sci., 39, 258- 279. Chong, M., and J. Testud , 1983: Three-Dimensional Wind Field Analysis from Dual-Doppler Radar Data. Part III: The Boundary

Formation and Expansion of Leukemia-Specific Chromosome Aberrations in Hematopoietic Cells of X-ray Irradiated Mice

International Nuclear Information System (INIS)

Ban, N.; Kai, M.; Kusama, T.

2004-01-01

C3H/He mice develop acute myeloid leukemia (AML) after whole-body irradiation, and typical chromosome 2 deletions are found in the leukemia cells. To investigate a process of the formation and the expansion of the AML-specific deletions, we have examined its frequency in primitive hematopoietic cells that could be the target of the leukemogenesis. Male C3H/He mice were exposed to 3Gy x-rays and sacrificed after certain periods of time. Bone marrow cells were collected from the femora and a single-cell suspension from each animal was divided into two parts. One part of the cell suspension was cultured in methylcellulose medium and metaphase spreads were prepared from each growing colony. The other part was sorted to obtain Lin+ and Lin Scal cells and those cells were scored with FISH for the AML-specific deletions. Karyotyping of the cultured cells detected signs of the delayed chromosomal instability, but an aberration involving chromosome 2 has not been found so far. FISH to the sorted cells, however, revealed the ANL-specific deletions could be produced in the primitive hematopoietic cells as an early event of radiation exposure. (Author) 16 refs

Structural properties of small Lin (n = 5-8) atomic clusters via ab initio random structure searching: A look into the role of different implementations of long-range dispersion corrections

Science.gov (United States)

Putungan, Darwin Barayang; Lin, Shi-Hsin

2018-01-01

In this work, we looked into the lowest energy structures of small lithium clusters (Lin, n = 5, 6, 7, 8) utilizing conventional PBE exchange-correlation functional, PBE with D2 dispersion correction and PBE with Tkatchenko and Scheffler (TS) dispersion correction, and searched using ab initio random structure searching. Results show that in general, dispersion-corrected PBE obtained similar lowest minima structures as those obtained via conventional PBE regardless of the type of implementation, although both D2 and TS found several high-energy isomers that conventional PBE did not arrive at, with TS in general giving more structures per energy range that could be attributed to its environment-dependent implementation. Moreover, D2 and TS dispersion corrections found a lowest energy geometry for Li8 cluster that is in agreement with the structure obtained via the typical benchmarking method diffusion Monte Carlo in a recent work. It is thus suggested that for much larger lithium clusters, utilization of dispersion correction could be of help in searching for lowest energy minima that is in close agreement with that of diffusion Monte Carlo results, but computationally inexpensive.

Temporal variation and source identification of black carbon at Lin'an and Longfengshan regional background stations in China

Science.gov (United States)

Cheng, Siyang; Wang, Yaqiang; An, Xingqin

2017-12-01

Black carbon (BC) is a component of fine particulate matter (PM2.5), associated with climate, weather, air quality, and people's health. However, studies on temporal variation of atmospheric BC concentration at background stations in China and its source area identification are lacking. In this paper, we use 2-yr BC observations from two background stations, Lin'an (LAN) and Longfengshan (LFS), to perform the investigation. The results show that the mean diurnal variation of BC has two significant peaks at LAN while different characteristics are found in the BC variation at LFS, which are probably caused by the difference in emission source contributions. Seasonal variation of monthly BC shows double peaks at LAN but a single peak at LFS. The annual mean concentrations of BC at LAN and LFS decrease by 1.63 and 0.26 μg m-3 from 2009 to 2010, respectively. The annual background concentration of BC at LAN is twice higher than that at LFS. The major source of the LAN BC is industrial emission while the source of the LFS BC is residential emission. Based on transport climatology on a 7-day timescale, LAN and LFS stations are sensitive to surface emissions respectively in belt or approximately circular area, which are dominated by summer monsoon or colder land air flows in Northwest China. In addition, we statistically analyze the BC source regions by using BC observation and FLEXible PARTicle dispersion model (FLEXPART) simulation. In summer, the source regions of BC are distributed in the northwest and south of LAN and the southwest of LFS. Low BC concentration is closely related to air mass from the sea. In winter, the source regions of BC are concentrated in the west and south of LAN and the northeast of the threshold area of s tot at LFS. The cold air mass in the northwest plays an important role in the purification of atmospheric BC. On a yearly scale, sources of BC are approximately from five provinces in the northwest/southeast of LAN and the west of LFS. These

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Science.gov (United States)

Lal, Rup; Pandey, Gunjan; Sharma, Pooja; Kumari, Kirti; Malhotra, Shweta; Pandey, Rinku; Raina, Vishakha; Kohler, Hans-Peter E.; Holliger, Christof; Jackson, Colin; Oakeshott, John G.

2010-01-01

Summary: Lindane, the γ-isomer of hexachlorocyclohexane (HCH), is a potent insecticide. Purified lindane or unpurified mixtures of this and α-, β-, and δ-isomers of HCH were widely used as commercial insecticides in the last half of the 20th century. Large dumps of unused HCH isomers now constitute a major hazard because of their long residence times in soil and high nontarget toxicities. The major pathway for the aerobic degradation of HCH isomers in soil is the Lin pathway, and variants of this pathway will degrade all four of the HCH isomers although only slowly. Sequence differences in the primary LinA and LinB enzymes in the pathway play a key role in determining their ability to degrade the different isomers. LinA is a dehydrochlorinase, but little is known of its biochemistry. LinB is a hydrolytic dechlorinase that has been heterologously expressed and crystallized, and there is some understanding of the sequence-structure-function relationships underlying its substrate specificity and kinetics, although there are also some significant anomalies. The kinetics of some LinB variants are reported to be slow even for their preferred isomers. It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation. PMID:20197499

A model for the Lin-Shu type density-wave structure of our Galaxy: Line-of-sight and transverse-longitudinal velocities of 242 optically visible open clusters

Science.gov (United States)

Griv, E.; Jiang, I.-G.

2015-02-01

In this paper, the fourth in a series, we examine again one of the implications of the Lin-Shu density-wave theory, specifically, the noncircular systematic motion of the Galactic objects. Our previous investigation is extended by analyzing simultaneously both the line-of-sight and transversal velocities of a sample of open clusters for which velocities, distances and ages are available. The ordinary equations of the Oort-Lindblad theory of galactic differential rotation are used. The minor effects caused by the two-dimensional tightly-wound density waves are also taken into account. The published data of 242 currently known optically visible clusters having distances rsight and transversal along the Galactic longitude velocities are nearly equal. We argue that the resemblance of these Galactic wave structures is so remarkable that no doubt is felt as to the theory's truth with respect to these data. The results obtained allow us to conclude that several low-m trailing density-wave patterns with different number of spiral arms m (say, m=1, 2, 3, and 4), pitch angles (about 5o, 8o, 11o, and 14o, respectively) and amplitudes of the perturbed gravitational potential may coexist in the Galaxy. The latter suggests the asymmetric multiarm, not well-organized (``flocculent'') spiral structure of the system. In memory of Professors Alexei M. Fridman (1940-2010) and Chi Yuan (1937-2008)

Blood-brain transfer and antinociception of linear and cyclic N-methyl-guanidine and thiourea-enkephalins

DEFF Research Database (Denmark)

Verbeken, Mathieu; Wynendaele, Evelien; Mauchauffee, Elodie

2015-01-01

Enkephalins are active in regulation of nociception in the body and are key in development of new synthetic peptide analogs that target centrally located opioid receptors. In this study, we investigated the in vivo blood–brain barrier (BBB) penetration behavior and antinociceptive activity of two...... cyclic enkephalin analogs with a thiourea (CycS) or a N-methyl-guanidine bridge (CycNMe), and their linear counterparts (LinS and LinNMe) in mice, as well as their in vitro metabolic stability. 125I-LinS had the highest blood–brain clearance (K1 = 3.46 μL/g min), followed by 125I-LinNMe, 125I...

Efimov effect in 2-neutron halo nuclei

Indian Academy of Sciences (India)

. B33, 563 (1970). [3] V Efimov, Comm. Nucl. Part. Phys. 19, 271 (1990). [4] Th Cornelius and W Glöckle, J. Chem. Phys. 85, 3906 (1986). B D Esry, C D Lin and C H Greene, Phys. Rev. A54, 394 (1996). J P D'Incao, H Suno and B D Esry, Phys.

Compiling Dictionaries

African Journals Online (AJOL)

Information Technology

This method results in a classified word list that can be efficiently ... standardized list of domains to classify multiple dictionaries opens up possibilities for cross-lin- .... part of speech, noun class, the plural form of each noun, and a simple gloss. ... But these mental links tend to cluster around a ... group, duet, trio, ensemble.

Prescription patterns of traditional Chinese medicine for peptic ulcer disease in Taiwan: A nationwide population-based study.

Science.gov (United States)

Huang, Chin-Ying; Lai, Wan-Yu; Sun, Mao-Feng; Lin, Che-Chen; Chen, Bor-Chyuan; Lin, Hung-Jen; Chang, Ching-Mao; Yang, Chung-Hsien; Huang, Kuo-Chin; Yen, Hung-Rong

2015-12-24

Peptic ulcer disease is a common digestive disease. There is a lack of large-scale survey on the use of traditional Chinese medicine (TCM) for the treatment of peptic ulcer disease. This study aimed to investigate the utilization of TCM for the treatment of peptic ulcer disease in Taiwan. We analyzed a random sample comprised of one million individuals with newly diagnosed peptic ulcer disease between 2001 and 2010 from the National Health Insurance Research Database in Taiwan. Demographic characteristics and TCM usage, including Chinese herbal formulas and the single herbs prescribed for patients with peptic ulcer disease, were analyzed. A total of 96,624 newly diagnosed subjects with peptic ulcer disease were included. 14,983 (15.5%) patients were TCM users. People residing in highly urbanized areas, younger people and female (compared with male) were more likely to use TCM. With regard to the comorbidities, TCM users had a lower prevalence of coronary artery disease, chronic obstructive lung disease, diabetes mellitus and liver cirrhosis and stroke. The average time between onset of peptic ulcer disease and the first visit to a TCM clinic was 4.7 months. Majority of the patients (n=14,449; 96.4%) received only Chinese herbal remedies. The most frequently prescribed Chinese herbal formula and single herb was Ban-Xia-Xie-Xin-Tang (Pinelliae Decoction to Drain the Epigastrium) and Hai-Piao-Xiao (Os Sepiae), respectively. The core pattern analysis showed that combination of Ban-Xia-Xie-Xin-Tang, Hai-Piao-Xiao (Os Sepiae), Yan-Hu-Suo (Rhizoma Corydalis), Bei-Mu (Bulbus Fritillariae Thunbergii) and Chuan-Lian-Zi (Fructus Toosendan) was most frequently used for peptic ulcer disease. Our study identified the core prescription patterns of TCM for patients with peptic ulcer disease in Taiwan. Further basic and clinical studies are necessary to elucidate the efficacy and mechanisms. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Planning by Using Digital Technology in the Reconstruction of Cultural Heritage Sites - a Case Study of Qiong-Lin Settlement in Kinmen Area

Science.gov (United States)

Yang, W. B.; Ye, Y. N.

2017-08-01

ICOMOS Florence Declaration in 2014, encourages an in-depth reflection on human values through cultural heritage and landscapes, which emphasizes the importance of historical heritage sites, in order to achieve the application of cultural heritage records through the public participation, sharing new technology platform and facilitation tools for knowledge diffusion, for instance. Nikos adopted digitized intangible cultural heritage within i-Treasures project to create a novel digital platform in 2016. Nowadays, the display platform developed based on geographic information system has been gradually accepted and widely used to distribute cultural heritage information, aiming to combine geography, time, events, issues, trends with the interactive maps to show the context of data changes from the consideration of planarity; for example, Burnaby City in Canada has cooperated with the Columbia University to create a navigation platform for guidance of tangible cultural heritage based on story maps in order to provide public recognition function. In this study, Qiong-Lin Settlement in Kinmen Area was taken as an example to illustrate the developing process of an overall planning framework for reappearing the glory of historic settlements of cultural heritage sites with digital technology, which included tangible and intangible cultural heritage preservation and transmission planning, community participation and digital navigation programs. The digital technology with the GIS-based digital platform can provide more diverse and interesting information while using an intuitive, graphical user story mapping interface. So that tangible cultural heritage can be effectively understood, interpreted and preserved with the value-added methods, and also intangible cultural heritage can be continuously transmitted to establish a complete system of cultural heritage preservation. The main contents include several navigation technologies, such as 3D laser scanning, UAV images

The pedagogical value of a student-run community-based experiential learning project: The Yong Loo Lin School of Medicine Public Health Screening.

Science.gov (United States)

Wee, Liang En; Yeo, Wei Xin; Tay, Clifton M; Lee, Jeannette J M; Koh, Gerald C H

2010-09-01

We assessed the pedagogical value of a student-led community-based experiential learning project called the Public Health Screening (PHS) run by medical and nursing students of the National University of Singapore's Yong Loo Lin School of Medicine (NUS YLLSoM). We conducted a cross-sectional study using a self-administered anonymised questionnaire on medical and nursing students who participated in PHS using the Fund for the Improvement of Postsecondary Education (FIPSE) Survey Instrument. Participants also gave an overall score for their learning experience at the PHS. The participation rate was 93.1% (576/619) for medical students and 100% (37/37) for nursing students. All participants gave the PHS learning experience a high rating (median = 8 out of maximum of 10, inter-quartile range, 7 to 9). A majority of participants felt that PHS had helped them to improve across all domains surveyed. For medical students, those in preclinical years and females were independently more likely to feel that PHS had helped them to improve in communication skills, teamwork, ability to identify social issues, taking action, and gaining and applying their knowledge than those in clinical years and males. Improved ability to interact with patients (β=1.64, 95%CI, 1.01-2.27), appreciation of challenges to healthcare faced by Singaporeans from lower income groups (β=0.93, 95%CI, 0.49-1.37), thinking of others (β=0.70, 95%CI, 0.04-1.37) and tolerance of different people (β =0.63, 95%CI, 0.17-1.10) were strongly associated with the overall rating score. PHS was a positive learning experience in a wide range of domains for all students involved. This suggests that student-organised community-based experiential learning projects have potential educational value for both medical and nursing students.

One-step hysteroscopic myomectomy using Lin dissecting loop and Lin myoma graspers

Directory of Open Access Journals (Sweden)

Bao-Liang Lin

2012-11-01

Conclusion: Using our technique and instruments, it is possible to remove a myoma in a single procedure safely and effectively. This surgical method can also preserve more endometrium, cause less injury to the muscle layer, and prevent fluid complications.

Population structure and biological characteristics of Siriella gracilis (Mysida) from the Exclusive Economic Zone of India

Digital Repository Service at National Institute of Oceanography (India)

Biju, A.; Honey, U.K.; Kusum, K.K.; Jagadeesan, L.

di ffe re nt se as o n s St at io n n o . La tit ud e (°N ) Lo ng itu de (°E ) IM S (20 04 ) SW M (20 05 ) N EM (20 06 ) S. gr a ci lis Te m pe ra tu re Sa lin ity S. gr a ci lis Te m pe ra tu re Sa lin ity S. gr a ci lis Te m pe ra tu re Sa lin ity... 04 ) SW M (20 05 ) N EM (20 06 ) S. gr a ci lis Te m pe ra tu re Sa lin ity S. gr a ci lis Te m pe ra tu re Sa lin ity S. gr a ci lis Te m pe ra tu re Sa lin ity de ns ity (°C ) de ns ity (°C ) de ns ity (°C ) 27 15 72 – 29 .7 35 .0 – 30 .7 35 .9...

N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, klf2, klf4, and lin28b.

Directory of Open Access Journals (Sweden)

Rebecca Cotterman

2009-06-01

Full Text Available myc genes are best known for causing tumors when overexpressed, but recent studies suggest endogenous myc regulates pluripotency and self-renewal of stem cells. For example, N-myc is associated with a number of tumors including neuroblastoma, but also plays a central role in the function of normal neural stem and precursor cells (NSC. Both c- and N-myc also enhance the production of induced pluripotent stem cells (iPSC and are linked to neural tumor stem cells. The mechanisms by which myc regulates normal and neoplastic stem-related functions remain largely open questions. Here from a global, unbiased search for N-Myc bound genes using ChIP-chip assays in neuroblastoma, we found lif as a putative N-Myc bound gene with a number of strong N-Myc binding peaks in the promoter region enriched for E-boxes. Amongst putative N-Myc target genes in expression microarray studies in neuroblastoma we also found lif and three additional important embryonic stem cell (ESC-related factors that are linked to production of iPSC: klf2, klf4, and lin28b. To examine the regulation of these genes by N-Myc, we measured their expression using neuroblastoma cells that contain a Tet-regulatable N-myc transgene (TET21N as well as NSC with a nestin-cre driven N-myc knockout. N-myc levels closely correlated with the expression of all of these genes in neuroblastoma and all but lif in NSC. Direct ChIP assays also indicate that N-Myc directly binds the lif promoter. N-Myc regulates trimethylation of lysine 4 of histone H3 in the promoter of lif and possibly in the promoters of several other stem-related genes. Together these findings indicate that N-Myc regulates overlapping stem-related gene expression programs in neuroblastoma and NSC, supporting a novel model by which amplification of the N-myc gene may drive formation of neuroblastoma. They also suggest mechanisms by which Myc proteins more generally contribute to maintenance of pluripotency and self-renewal of ESC as

A Hybrid Distance-Based Ideal-Seeking Consensus Ranking Model

Directory of Open Access Journals (Sweden)

Madjid Tavana

2007-01-01

Full Text Available Ordinal consensus ranking problems have received much attention in the management science literature. A problem arises in situations where a group of k decision makers (DMs is asked to rank order n alternatives. The question is how to combine the DM rankings into one consensus ranking. Several different approaches have been suggested to aggregate DM responses into a compromise or consensus ranking; however, the similarity of consensus rankings generated by the different algorithms is largely unknown. In this paper, we propose a new hybrid distance-based ideal-seeking consensus ranking model (DCM. The proposed hybrid model combines parts of the two commonly used consensus ranking techniques of Beck and Lin (1983 and Cook and Kress (1985 into an intuitive and computationally simple model. We illustrate our method and then run a Monte Carlo simulation across a range of k and n to compare the similarity of the consensus rankings generated by our method with the best-known method of Borda and Kendall (Kendall 1962 and the two methods proposed by Beck and Lin (1983 and Cook and Kress (1985. DCM and Beck and Lin's method yielded the most similar consensus rankings, whereas the Cook-Kress method and the Borda-Kendall method yielded the least similar consensus rankings.

Saúde mental no Sistema Único de Saúde: mudança do modelo de atenção na região de Lins-SP Mental health in Sistema Único de Saúde: changing the care model in the region of Lins - SP

Directory of Open Access Journals (Sweden)

Carlos Cesar Escudeiro

2009-03-01

Full Text Available Omunicípio de Linspassou portransformações significativas na atenção em Saúde Mental, evoluindo deum modelo de referência hospitalar para um modelo de base territorial e comunitária. Este trabalho tem como objetivo realizar uma retrospectiva históricadas mudanças ocorridas, descrevendoas ações de assistência, promoção, prevenção e reabilitação psicossocial, através da implantação deserviços substitutivos integrados à atenção básica, apontando as soluções capazes de efetivar os princípios do Sistema Único de Saúde (SUS. Priorizou-se a implantação dos Centros de atenção Psicossocial que são dispositivos estratégicos para favorecer a inserção social. A Gestão Municipal realizou significativos investimentos técnicos e financeiros que possibilitaram a consolidação da Política Nacional de Saúde Mental, ampliando as articulações intersetoriais.The municipality of Lins, in the state of São Paulo, has undergone significant transformations in Mental Health Care, evolving from a hospital reference model to a territorial and community-based model. This work aims to carry out a historical retrospective of the changes that have occurred, describing the actions of psychosocial assistance, promotion, prevention and rehabilitation through the implementation of substitutive services integrated into primary care, pointing to the solutions that can fulfill the principles of Sistema Único de Saúde (SUS - National Health System. The priority was the implementation of Centros de Atenção Psicossocial (Psychosocial Care Centers, which are strategic devices to favor social insertion. The Municipal Management has made significant technical and financial investments that have enabled the consolidation of the National Mental Health Policy, extending inter-sector articulations.

Therapeutic effects of Laser and L-carnitine against amiodarone ...

African Journals Online (AJOL)

Pulmonary toxicity is considered a life- threatening complication of ... Research Center, Faculty of Medicine, Ain .... Laser therapy restores the balance between oxidants and ... No conflict of interest associated with this work. Contribution of .... Fitness. 2014; 149-159. 21. Lee BJ, Lin JS, Lin YC, Lin PT. Effects of L-carnitine.

Production and characterization of nanocapsules encapsulated linalool by ionic gelation method using chitosan as wall material

Directory of Open Access Journals (Sweden)

Zuobing XIAO

Full Text Available Abstract Linalool has been extensively applied in various fields, such as flavoring agent, perfumes, cosmetics and medical science. However, linalool is unstable, volatile and readily oxidizable. A sensitive substance can be encapsulated in a capsule, so encapsulation technology can solve these problems. In this paper, linalool-loaded nanocapsules (Lin-nanocapsules were prepared via the ionic gelation method and Lin-nanocapsules were characterized. The results of Fourier transformation infrared spectroscopy (FTIR showed that linalool was successfully encapsulated in the wall materials. Scanning electron microscopy (SEM results demonstrated that the shapes of Lin-nanocapsules, with smooth surfaces, were nearly spherical. Lin-nanocapsule average particle size was 352 nm and its polydispersity index (PDI was proved to be 0.214 by the results of dynamic light scattering (DLC. Thermogravimetric results indicated that linalool loading capacity (LC was 15.17%, and encapsulation could decrease linalool release and increase linalool retaining time under the high temperature. Oscillatory shear and steady-state shear measurements of Lin-nanocapsule emulsions were systematically investigated. The results of steady-state shear showed that Lin-nanocapsule emulsion, which was Newtonian only for high shear rate, was non-Newtonian. It was proved by oscillatory shear that when oscillation frequency changed from low to high, Lin-nanocapsules emulsion changed from viscous into elastic.

Smart Methods for Linezolid Determination in the Presence of Alkaline and Oxidative Degradation Products Utilizing Their Overlapped Spectral Bands

Science.gov (United States)

Abd El-Monem Hegazy, M.; Shaaban Eissa, M.; Abd El-Sattar, O. I.; Abd El-Kawy, M. M.

2014-09-01

Linezolid (LIN) is considered the first available oxazolidinone antibacterial agent. It is susceptible to hydrolysis and oxidation. Five simple, accurate, sensitive and validated UV spectrophotometric methods were developed for LIN determination in the presence of its alkaline (ALK) and oxidative (OXD) degradation products in bulk powder and pharmaceutical formulation. Method A is a second derivative one (D2) in which LIN is determined at 240.9 nm. Method B is a pH-induced differential derivative one where LIN is determined using the fourth derivative (D4) of the difference spectra (ΔA) at 285.3 nm. Methods C, D, and E are manipulating ratio spectra, where C is the double divisor-ratio difference spectrophotometric one (DD-RD) in which LIN was determined by calculating the amplitude difference at 243.7 and 267.6 nm of the ratio spectra. Method D is the double divisor-first derivative of ratio spectra (DD-DD1) in which LIN was determined at 270.2 nm. Method E is a mean centering of ratio spectra one (MCR) in which LIN was determined at 318.0 nm. The developed methods have been validated according to ICH guidelines. The results were statistically compared to that of a reported HPLC method and there was no significant difference regarding both accuracy and precision.

Untitled

Indian Academy of Sciences (India)

We have checked that the long range (u, wು) and short Tangರಿ (M, W) parts of the wavefunction match exactly at r = b. The functions I,(x) (n = 1/2, 3/25/2) appearing in the above expressions, are the. MOdified Spherical Bessel functions of Second kind, whereas K's are expressible as. linCar Combinations of Second and ...

Horizontal subsurface flow constructed wetlands for mitigation of ...

African Journals Online (AJOL)

2008-12-22

Dec 22, 2008 ... Results indicated that 39% of the total initially added amount of ametryn ... characteristics (Table 1), may result in the detection of poten- ... Water solubility at 20oC (g∙m-3) 204 ... culture, part of the resident liquid in CWs was lost, therefore ..... LIN T, WEN y, JIANG L, LI J, yANG S and ZHOu Q (2008) Study.

Caenorhabditis elegans histone deacetylase hda-1 is required for morphogenesis of the vulva and LIN-12/Notch-mediated specification of uterine cell fates.

Science.gov (United States)

Ranawade, Ayush Vasant; Cumbo, Philip; Gupta, Bhagwati P

2013-08-07

Chromatin modification genes play crucial roles in development and disease. In Caenorhabditis elegans, the class I histone deacetylase family member hda-1, a component of the nucleosome remodeling and deacetylation complex, has been shown to control cell proliferation. We recovered hda-1 in an RNA interference screen for genes involved in the morphogenesis of the egg-laying system. We found that hda-1 mutants have abnormal vulva morphology and vulval-uterine connections (i.e., no uterine-seam cell). We characterized the vulval defects by using cell fate-specific markers and found that hda-1 is necessary for the specification of all seven vulval cell types. The analysis of the vulval-uterine connection defect revealed that hda-1 is required for the differentiation of the gonadal anchor cell (AC), which in turn induces ventral uterine granddaughters to adopt π fates, leading to the formation of the uterine-seam cell. Consistent with these results, hda-1 is expressed in the vulva and AC. A search for hda-1 target genes revealed that fos-1 (fos proto-oncogene family) acts downstream of hda-1 in vulval cells, whereas egl-43 (evi1 proto-oncogene family) and nhr-67 (tailless homolog, NHR family) mediate hda-1 function in the AC. Furthermore, we showed that AC expression of hda-1 plays a crucial role in the regulation of the lin-12/Notch ligand lag-2 to specify π cell fates. These results demonstrate the pivotal role of hda-1 in the formation of the vulva and the vulval-uterine connection. Given that hda-1 homologs are conserved across the phyla, our findings are likely to provide a better understanding of HDAC1 function in development and disease.

PLANNING BY USING DIGITAL TECHNOLOGY IN THE RECONSTRUCTION OF CULTURAL HERITAGE SITES – A CASE STUDY OF QIONG-LIN SETTLEMENT IN KINMEN AREA

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W. B. Yang

2017-08-01

Full Text Available ICOMOS Florence Declaration in 2014, encourages an in-depth reflection on human values through cultural heritage and landscapes, which emphasizes the importance of historical heritage sites, in order to achieve the application of cultural heritage records through the public participation, sharing new technology platform and facilitation tools for knowledge diffusion, for instance. Nikos adopted digitized intangible cultural heritage within i-Treasures project to create a novel digital platform in 2016. Nowadays, the display platform developed based on geographic information system has been gradually accepted and widely used to distribute cultural heritage information, aiming to combine geography, time, events, issues, trends with the interactive maps to show the context of data changes from the consideration of planarity; for example, Burnaby City in Canada has cooperated with the Columbia University to create a navigation platform for guidance of tangible cultural heritage based on story maps in order to provide public recognition function. In this study, Qiong-Lin Settlement in Kinmen Area was taken as an example to illustrate the developing process of an overall planning framework for reappearing the glory of historic settlements of cultural heritage sites with digital technology, which included tangible and intangible cultural heritage preservation and transmission planning, community participation and digital navigation programs. The digital technology with the GIS-based digital platform can provide more diverse and interesting information while using an intuitive, graphical user story mapping interface. So that tangible cultural heritage can be effectively understood, interpreted and preserved with the value-added methods, and also intangible cultural heritage can be continuously transmitted to establish a complete system of cultural heritage preservation. The main contents include several navigation technologies, such as 3D laser scanning

In reactor measurements, modeling and assessments to predict liquid injection shutdown system nozzle to Calandria tube time to contact

International Nuclear Information System (INIS)

Kirstein, K.; Kalenchuk, D.

2011-01-01

Over the past few years there has been an expanding effort to assess the potential for Calandria Tubes (CTs) coming into contact with Liquid Injection Shutdown System (LISS) Nozzles to ensure continued contact-free operation as required by CSA N285.4. LISS Nozzles (LINs), which run perpendicular to and between rows of fuel channels, sag at a slower rate than the fuel channels. As a result certain LINs may come in contact with CTs above them. The CT/LIN gaps can be predicted from calculated CT sag, LIN sag and a number of component and installation tolerances. This method however results in very conservative predictions when compared to measurements, confirmed with the in reactor measurements initiated in 2000, when gaps were successfully measured the first time using images obtained from a camera-assisted measurement tool inserted into the calandria. To reduce the conservatism of the CT/LIN gap predictions, statistical CT/LIN gap models are used instead. They are derived from a comparison between calculated gaps based on nominal dimensions and the visual image based measured gaps. These reactor specific (typically 95% confidence level) CT/LIN gap models account for all uncertainties and deviations from nominal values. Prediction error margins reduce as more in-reactor gap measurements become available. Each year more measurements are being made using this standardized visual CT/LIN proximity method. The subsequently prepared reactor-specific models have been used to provide time to contact for every channel above the LINs at these stations. In a number of cases it has been used to demonstrate that the reactor can be operated to its end of life before refurbishment with no predicted contact, or specific at-risk channels have been identified for which appropriate remedial actions could be implemented in a planned manner. (author)

Pharmacological Characterization of 5-Substituted 1-[(2,3-dihydro-1-benzofuran-2-ylmethyl]piperazines: Novel Antagonists for the Histamine H3 and H4 Receptors with Anti-inflammatory Potential

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Michelle F. Corrêa

2017-11-01

Full Text Available The histamine receptors (HRs are traditional G protein-coupled receptors of extensive therapeutic interest. Recently, H3R and H4R subtypes have been targeted in drug discovery projects for inflammation, asthma, pain, cancer, Parkinson’s, and Alzheimer’s diseases, which includes searches for dual acting H3R/H4R ligands. In the present work, nine 1-[(2,3-dihydro-1-benzofuran-2-ylmethyl]piperazine (LINS01 series molecules were synthesized and evaluated as H3R and H4R ligands. Our data show that the N-allyl-substituted compound LINS01004 bears the highest affinity for H3R (pKi 6.40, while the chlorinated compound LINS01007 has moderate affinity for H4R (pKi 6.06. In addition, BRET assays to assess the functional activity of Gi1 coupling indicate that all compounds have no intrinsic activity and act as antagonists of these receptors. Drug-likeness assessment indicated these molecules are promising leads for further improvements. In vivo evaluation of compounds LINS01005 and LINS01007 in a mouse model of asthma showed a better anti-inflammatory activity of LINS01007 (3 g/kg than the previously tested compound LINS01005. This is the first report with functional data of these compounds in HRs, and our results also show the potential of their applications as anti-inflammatory.

Essential Oil of Ocimum basilicum L. and (−-Linalool Blocks the Excitability of Rat Sciatic Nerve

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Antonio Medeiros Venancio

2016-01-01

Full Text Available The racemate linalool and its levogyrus enantiomer [(−-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of the Ocimum basilicum L. (EOOb derived from Germplasm Bank rich in (−-LIN content in the excitability of peripheral nervous system were studied. We used rat sciatic nerve to investigate the EOOb and (−-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP. EOOb and (−-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout. EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50 of 0.38±0.2 and 0.17±0.0 mg/mL, respectively. For (−-LIN, these values were 0.23±0.0 and 0.13±0.0 mg/mL. Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component. In conclusion EOOb and (−-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (−-LIN in the essential oil.

SKR-1, a homolog of Skp1 and a member of the SCFSEL-10 complex, regulates sex-determination and LIN-12/Notch signaling in C. elegans

Science.gov (United States)

Killian, Darrell J.; Harvey, Elizabeth; Johnson, Peter; Otori, Muneyoshi; Mitani, Shohei; Xue, Ding

2008-01-01

Sex-determination in C. elegans requires regulation of gene transcription and protein activity and stability. sel-10 encodes a WD40-repeat-containing F-box protein that likely mediates the ubiquitin-mediated degradation of important sex-determination factors. Loss of sel-10 results in a mild masculinization of hermaphrodites, whereas dominant alleles of sel-10, such as sel-10(n1074), cause a more severe masculinization, including a reversal of the life versus death decision in sex-specific neurons. To investigate about how sel-10 regulates sex-determination, we conducted a sel-10(n1074) suppressor screen and isolated a weak loss-of-function allele of skr-1, one of 21 Skp1-related genes in C. elegans. Skp1, Cullin, and F-box proteins, such as SEL-10, are components of the SCF E3 ubiquitin ligase complex. We present genetic evidence that the sel-10(n1074) masculinization phenotype is dependent upon skr-1 and cul-1 activity. Furthermore, we show that the SKR-1(M140I) weak loss-of-function mutation interferes with SKR-1/SEL-10 binding. Unexpectedly, we found that the G567E substitution in SEL-10 caused by the n1074 allele impairs the binding of SEL-10 to SKR-1 and the dimerization of SEL-10, which may be important for SEL-10 function. Our results suggest that SKR-1, CUL-1 and SEL-10 constitute an SCF E3 ligase complex that plays an important role in modulating sex-determination and LIN-12/Notch signaling in C. elegans. PMID:18718460

WYSTĘPOWANIE ROŚLIN INWAZYJNYCH W OBRĘBIE BUDOWLI I POWIERZCHNI UTWARDZONYCH W DOLINACH RZECZNYCH KARPAT I KOTLINY SANDOMIERSKIEJ

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Dominik WRÓBEL

Full Text Available Badania terenowe, prowadzone w latach 2010-2016, w dolinach rzecznych polskiej części Karpat oraz w Kotlinie Sandomierskiej i w przylegającym do niej odcinku doliny Wisły, miały za zadanie uzupełnić, wiedzę o występowaniu inwazyjnych gatunków roślin (inwaderów w najsilniej przekształconych dolinach rzecznych, a w szczególności określić typy zabudowy dolin rzecznych, sprzyjające rozprzestrzenianiu się tych gatunków. Przeanalizowano 118 transektów zlokalizowanych zarówno w regionach górskich, podgórskich i nizinnych, w odcinkach uregulowanych jak i nieuregulowanych dolin rzecznych, cieków o różnej wielkości. Wyodrębniono główne typy/kategorie zabudowy, łączące w sobie: obiekty hydrotechniczne i przeciwpowodziowe, w tym obwałowania, umocnienia brzegowe i ostrogi korytowe (I, mieszkalną i usługową zabudowę śródmiejską (II, drogowe i kolejowe linie komunikacyjne, w tym mosty (III, wyrobiska górnicze, zabudowę produkcyjną, wydobywczą, magazynową i towarzyszącą (IV, zabudowę rozproszoną, ogródki działkowe (V oraz odrębne place, parkingi i składowiska (VI. Na częściach transektów, obejmujących różne formy zabudowy, najczęściej zanotowano występowanie Solidago gigantea / S. canadensis (46, Impatiens glandulifera (30, Echinocystis lobata (22, Robinia pseudoacacia (17, Helianthus tuberosus (15 i Impatiens parviflora (15. Największa liczba stanowisk gatunków inwazyjnych w relacji do wszystkich ich stwierdzeń została zanotowana na różnego rodzaju budowlach hydrotechnicznych, w tym na umocnieniach brzegowych różnego typu. Obserwacje prowadzone w zakresie wpływu inwestycji regulacyjnych na szatę roślinną wskazują, że nie ma istotnych różnic co do zastosowanych sposobów zabudowy umocnieniowej brzegów, które można byłoby uznać za bardziej przyjazne środowisku. W każdym przypadku następuje pozostawianie odkrytego podłoża i promowanie wkraczania inwaderów.

A Fully Analytical Solution of the Wellbore Stability Problem under Undrained Conditions Using a Linearised Cam-Clay Model Solution analytique au problème de stabilité de puits en conditions non drainées utilisant un modèle de Cam-Clay linéarisé

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Charlez P. A.

2006-12-01

Full Text Available This paper presents a linearised version of the Cam-Clay model fully integrated in the scope of the general theory of poroplasticity. The constitutive law which is developed in the scope of the effective plastic stress concept, only contains two plastic parameters (hardening modulus and slope of the critical state line. To be validated, the model is integrated over homogeneous stress paths (hydrostatic, drained triaxial and undrained triaxal then compared with experimental data issued from conventional laboratory triaxial tests. In the second part, a simplified version of the model is applied to the wellbore boundary problem (vertical well in an axisymmetric horizontal stress field and under undrained conditions. Given the linearity of the constitutive law and the a priori knowledge of the shape of the plastic region, the solution (stress, strain and pore pressure is fully analytical. The solution shows that for an overconsolidated material (overconsolidation degree less than 2 the hoop stress is strongly relaxed in the plastic zone. The higher the compressibility of the saturating fluid, the larger the relaxation of the hoop stress. In terms of stability, the more compressible the fluid saturating the porous medium is, the more stable the well will be. Finally the larger the overconsolidation ratio is, the less stable the well will be. Cet article présente une version linéarisée du modèle de Cam-Clay intégré dans le cadre général de la théorie de la poroplasticité isotherme. La loi de comportement développée à partir du concept des contraintes effectives plastiques ne contient que deux paramètres plastiques (module d'écrouissage et pente de la droite critique. Le modèle est validé sur des chemins de contrainte homogènes (hydrostatique, triaxial drainé et triaxial non drainé et comparé à des essais expérimentaux obtenus à partir d'essais conventionnels de laboratoire. Ensuite, une version simplifiée du modèle est

A developmental timing switch promotes axon outgrowth independent of known guidance receptors.

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Katherine Olsson-Carter

2010-08-01

Full Text Available To form functional neuronal connections, axon outgrowth and guidance must be tightly regulated across space as well as time. While a number of genes and pathways have been shown to control spatial features of axon development, very little is known about the in vivo mechanisms that direct the timing of axon initiation and elongation. The Caenorhabditis elegans hermaphrodite specific motor neurons (HSNs extend a single axon ventrally and then anteriorly during the L4 larval stage. Here we show the lin-4 microRNA promotes HSN axon initiation after cell cycle withdrawal. Axons fail to form in lin-4 mutants, while they grow prematurely in lin-4-overexpressing animals. lin-4 is required to down-regulate two inhibitors of HSN differentiation--the transcriptional regulator LIN-14 and the "stemness" factor LIN-28--and it likely does so through a cell-autonomous mechanism. This developmental switch depends neither on the UNC-40/DCC and SAX-3/Robo receptors nor on the direction of axon growth, demonstrating that it acts independently of ventral guidance signals to control the timing of HSN axon elongation.

New Approaches for Prostate Cancer Combination Therapy

Science.gov (United States)

2009-04-01

Freemont AJ, et al. E - cadherin and h-catenin are down-regulated in prostatic bone metastases. BJU Int 2002;89:400–3. ...ola r Di clo fen ac 20 Di clo fen ac 10 Di clo fen ac 5 Su lin da c S ulf on e 2 00 Su lin da c S ulf on e 1 00 Su lin da c S ulf on e 5 0 Su...lin da c S ulf on e 2 5 Flu rb ipr ofe n 2 0 Flu rb ipr ofe n 1 0 Flu rb ipr ofe n 5 Flu rb ipr ofe n 2 As pir

Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors.

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Seo Hee Choi

Full Text Available To identify prognostic factors and define the optimal management of patients with supratentorial primitive neuroectodermal tumors (sPNETs, we investigated treatment outcomes and explored the prognostic value of specific molecular markers.A total of 47 consecutive patients with pathologically confirmed sPNETs between May 1985 and June 2012 were included. Immunohistochemical analysis of LIN28, OLIG2, and Rad51 expression was performed and correlated with clinical outcome.With a median follow-up of 70 months, 5-year overall survival (OS and progression-free survival (PFS was 55.5% and 40%, respectively, for all patients. Age, surgical extent, and radiotherapy were significant prognostic factors for OS and PFS. Patients who received initially planned multimodal treatment without interruption (i.e., radiotherapy and surgery (≥subtotal resection, with or without chemotherapy showed significantly higher 5-year OS (71.2% and PFS (63.1%. In 29 patients with available tumor specimens, tumors with high expression of either LIN28 or OLIG2 or elevated level of Rad51 were significantly associated with poorer prognosis.We found that multimodal treatment improved outcomes for sPNET patients, especially when radiotherapy and ≥subtotal resection were part of the treatment regimen. Furthermore, we confirmed the prognostic significance of LIN28 and OLIG2 and revealed the potential role of Rad51 in sPNETs.

An Analysis of Navy Nurse Corps Accession Sources

Science.gov (United States)

2014-03-01

1 I. INTRODUCTION A. BACKGROUND Since 1998, the United States has experienced a nationwide nursing shortage (Juraschek, Zhang, Ranganathan ...supply of RNs in the civilian workforce (Juraschek, Zhang, Ranganathan , & Lin, 2012). A study by Juraschek, Zhang, Ranganathan , & Lin (2012...Postgraduate School, Monterey, CA. Retrieved from www.dtic.mil/cgi- bin/GetTRDoc?AD=ADA344570 Juraschek, S. P., Zhang, X., Ranganathan , V. K., & Lin, V

Dicty_cDB: SLG867 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available PFFKSNLN*lin**lssgci*nvnnn**liixxxxiiiiimiiynn***qfffv lfvifypliyfpr Translated Amino Acid sequence (All Fra...IS NFNNNSPFFKSNLN*lin**lssgci*nvnnn**liixxxxiiiiimiiynn***qfffv lfvifypliyfpr Hom

Use of shell model calculations in R-matrix studies of neutron-induced reactions

International Nuclear Information System (INIS)

Knox, H.D.

1986-01-01

R-matrix analyses of neutron-induced reactions for many of the lightest p-shell nuclei are difficult due to a lack of distinct resonance structure in the reaction cross sections. Initial values for the required R-matrix parameters, E,sub(lambda) and γsub(lambdac) for states in the compound system, can be obtained from shell model calculations. In the present work, the results of recent shell model calculations for the lithium isotopes have been used in R-matrix analyses of 6 Li+n and 7 Li+n reactions for E sub(n) 7 Li and 8 Li on the 6 Li+n and 7 Li+n reaction mechanisms and cross sections are discussed. (author)

WRKY Transcription Factors: Key Components in Abscisic Acid Signaling

Science.gov (United States)

2011-01-01

networks that take inputs from numerous stimuli and that they are involved in mediating responses to numerous phytohormones including salicylic acid ... jasmonic acid , ABA and GA. These roles in multiple signalling pathways may in turn partly explain the pleiotropic effects commonly seen when TF genes are...Review article WRKY transcription factors: key components in abscisic acid signalling Deena L. Rushton1, Prateek Tripathi1, Roel C. Rabara1, Jun Lin1

Electromagnetic analysis of ITER generic equatorial port plug designs during three plasma current disruption cases

International Nuclear Information System (INIS)

Guirao, J.; Rodríguez, E.; Ordieres, J.; Cabanas, M.F.; García, C.H. Rojas

2012-01-01

Highlights: ► Electromagnetic transient performance evaluation of the GEPP structure. ► Three different plasma current disruption cases: MD UP LIN36, VDE UP LIN36 and VDE DW LIN36 were analyzed. ► Three DSM-First Wall (FW) designs (horizontal and vertical drawers and monoblock) were compared. - Abstract: Electromagnetic phenomena due to plasma current disruptions are the cause for the main mechanical operation loads over the ITER equatorial level port plug structures. This paper presents a detailed finite element simulation and analysis of the transient electromagnetic effects of three different plasma current disruption cases over three designs of diagnostic shielding module (DSM) structure. The DSMs are contained into and supported by the generic equatorial port plug (GEPP) analyzed structure. The three plasma disruption cases studied were: major disruption upwards linear decay in 36 ms (MD UP LIN36), vertical displacements events, upwards and downwards linear decay in 36 ms (VDE UP LIN36 and VDE DW LIN36). A detailed analysis for GEPP structure and three DSM-first wall (FW) designs (horizontal and vertical drawers and monoblock) is also presented in order to extract the Eddy current distribution on these devices and thus the resultant electromagnetic forces and moments acting on them.

REASONING OF LIBERAL ISLAM: A STUDY ON ISLAMIC PERSPECTIVES IN LIBERAL ISLAM NETWORK JAKARTA

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Yusa' Farchan

2016-12-01

Full Text Available The renewal of Islamic thinking continuously develops from time to time in Indonesia. This research tries to answer two main problems, they are: how are the reasoning or ideology developed by Liberal Islam Network (LIN?; and how are LIN’s main ideas and their metamorphoses? The research applies qualitative method with hermeneutic approach. The data is collected by library research or text documentation. The data is collected on August 18th to August 29th 2015. The research finds that the ideas of Liberal Islam Network show significant liberal signs. From the ontology of text, in reading religious text, LIN’s assumptions are “the critics over the truth”. LIN applies humanistic approach in reading the religious texts. This is confirmed by LIN’s defense toward human’s dignity. From the aspect of theoretically analytical tools in reading religious texts, LIN applies social sciences, which are socio-historical analyses. LIN uses symbol or semiotics analyses to read the religious text. The main notions of LIN are: First, Islam changes, it is not static; second, context is the first and history is the latter. Here, in reading a religious text, LIN wants to place context (socio-history proportionally; third, LIN intends to hold God in our ground and history. The metamorphoses of LIN’s ideas are: firstly is to oppose theocracy; secondly is to endorse democracy; thirdly is to endorse gender equality; fourthly is to contextualize religious doctrines; and fifthly is to support pluralism and freedom of thinking.

The influence of BDNF on human umbilical cord blood stem/progenitor cells: implications for stem cell-based therapy of neurodegenerative disorders.

Science.gov (United States)

Paczkowska, Edyta; Łuczkowska, Karolina; Piecyk, Katarzyna; Rogińska, Dorota; Pius-Sadowska, Ewa; Ustianowski, Przemysław; Cecerska, Elżbieta; Dołęgowska, Barbara; Celewicz, Zbigniew; Machaliński, Bogusław

2015-01-01

Umbilical cord blood (UCB)-derived stem/progenitor cells (SPCs) have demonstrated the potential to improve neurologic function in different experimental models. SPCs can survive after transplantation in the neural microenvironment and indu ce neuroprotection, endogenous neurogenesis by secreting a broad repertoire of trophic and immunomodulatory cytokines. In this study, the influence of brain-derived neurotrophic factor (BDNF) pre-treatment was comprehensively evaluated in a UCB-derived lineage-negative (Lin-) SPC population. UCB-derived Lin- cells were evaluated with respect to the expression of (i) neuronal markers using immunofluorescence staining and (ii) specific (TrkB) receptors for BDNF using flow cytometry. Next, after BDNF pre-treatment, Lin- cells were extensively assessed with respect to apoptosis using Western blotting and proliferation via BrdU incorporation. Furthermore, NT-3 expression levels in Lin- cells using RQ PCR and antioxidative enzyme activities were assessed. We demonstrated neuronal markers as well as TrkB expression in Lin- cells and the activation of the TrkB receptor by BDNF. BDNF pre-treatment diminished apoptosis in Lin- cells and influenced the proliferation of these cells. We observed significant changes in antioxidants as well as in the increased expression of NT-3 in Lin- cells following BDNF exposure. Complex global miRNA and mRNA profiling analyses using microarray technology and GSEA revealed the differential regulation of genes involved in the proliferation, gene expression, biosynthetic processes, translation, and protein targeting. Our results support the hypothesis that pre-treatment of stem/progenitor cells could be beneficial and may be used as an auxiliary strategy for improving the properties of SPCs.

C. elegans microRNAs.

Science.gov (United States)

Vella, Monica C; Slack, Frank J

2005-09-21

MicroRNAs (miRNAs) are small, non-coding regulatory RNAs found in many phyla that control such diverse events as development, metabolism, cell fate and cell death. They have also been implicated in human cancers. The C. elegans genome encodes hundreds of miRNAs, including the founding members of the miRNA family lin-4 and let-7. Despite the abundance of C. elegans miRNAs, few miRNA targets are known and little is known about the mechanism by which they function. However, C. elegans research continues to push the boundaries of discovery in this area. lin-4 and let-7 are the best understood miRNAs. They control the timing of adult cell fate determination in hypodermal cells by binding to partially complementary sites in the mRNA of key developmental regulators to repress protein expression. For example, lin-4 is predicted to bind to seven sites in the lin-14 3' untranslated region (UTR) to repress LIN-14, while let-7 is predicted to bind two let-7 complementary sites in the lin-41 3' UTR to down-regulate LIN-41. Two other miRNAs, lsy-6 and mir-273, control left-right asymmetry in neural development, and also target key developmental regulators for repression. Approximately one third of the C. elegans miRNAs are differentially expressed during development indicating a major role for miRNAs in C. elegans development. Given the remarkable conservation of developmental mechanism across phylogeny, many of the principles of miRNAs discovered in C. elegans are likely to be applicable to higher animals.

Strengthening the case that elevated levels of programmed death ligand 1 predict poor prognosis in hepatocellular carcinoma patients

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Zhong J

2016-12-01

Full Text Available Jian-Hong Zhong,1,* Cheng-Piao Luo,2,* Chun-Yan Zhang,2 Le-Qun Li1 1Hepatobiliary Surgery Department, 2Experimental Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, People’s Republic of China *These authors contributed equally to this work Abstract: Immunotherapy targeting programmed death receptor 1 and programmed death ligand 1 (PD-L1 has shown impressive antitumor efficacy in several solid cancers, including advanced hepatocellular carcinoma (HCC. Since response rates of various cancers to such immunotherapy appear to correlate with PD-L1 expression levels, several studies have examined whether PD-L1 expression correlates with HCC pathology and patient prognosis. In this paper, we analyzed the strength and limitations of a recent meta-analysis of associations of PD-L1 with HCC characteristics and patient prognosis. Keywords: hepatocellular carcinoma, programmed death ligand 1, hepatic resection, prognoses

Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis.

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Michalis Barkoulas

2016-09-01

Full Text Available Patterning of C. elegans vulval cell fates relies on inductive signaling. In this induction event, a single cell, the gonadal anchor cell, secretes LIN-3/EGF and induces three out of six competent precursor cells to acquire a vulval fate. We previously showed that this developmental system is robust to a four-fold variation in lin-3/EGF genetic dose. Here using single-molecule FISH, we find that the mean level of expression of lin-3 in the anchor cell is remarkably conserved. No change in lin-3 expression level could be detected among C. elegans wild isolates and only a low level of change-less than 30%-in the Caenorhabditis genus and in Oscheius tipulae. In C. elegans, lin-3 expression in the anchor cell is known to require three transcription factor binding sites, specifically two E-boxes and a nuclear-hormone-receptor (NHR binding site. Mutation of any of these three elements in C. elegans results in a dramatic decrease in lin-3 expression. Yet only a single E-box is found in the Drosophilae supergroup of Caenorhabditis species, including C. angaria, while the NHR-binding site likely only evolved at the base of the Elegans group. We find that a transgene from C. angaria bearing a single E-box is sufficient for normal expression in C. elegans. Even a short 58 bp cis-regulatory fragment from C. angaria with this single E-box is able to replace the three transcription factor binding sites at the endogenous C. elegans lin-3 locus, resulting in the wild-type expression level. Thus, regulatory evolution occurring in cis within a 58 bp lin-3 fragment, results in a strict requirement for the NHR binding site and a second E-box in C. elegans. This single-cell, single-molecule, quantitative and functional evo-devo study demonstrates that conserved expression levels can hide extensive change in cis-regulatory site requirements and highlights the evolution of new cis-regulatory elements required for cell-specific gene expression.

[Pollution characteristics and ecological risk assessment of heavy metals in surface sediments of Qingshan Reservoir in Lin' an City, Zhejiang Province of East China].

Science.gov (United States)

Zhang, Fen; Yang, Chang-Ming; Pan, Rui-Jie

2013-09-01

A total of 8 representative surface sediment sampling sites were collected from the Qingshan Reservoir in Lin'an City of Zhejiang Province to investigate the differences in the total concentrations of As, Cr, Cu, Ni, Mn, Pb, and Zn among the sampling sites. The different forms of the heavy metals, i. e., acid soluble, easily reducible, easily oxidizable, and residual, were determined by BCR sequential extraction method, and the pollution degrees and potential ecological risk, of the heavy metals in the surface sediments at different sampling sites of the Reservoir were assessed by using geo-accumulation index (I(geo)) and Hakanson potential ecological risk index. There existed obvious spatial differences in the total concentrations of the heavy metals in the surface sediments of the Reservoir. The sampling sites nearby the estuaries of the tributaries flowing through downtowns and heavy industrial parks to the Reservoir had obviously higher heavy metals concentrations in surface sediments, as compared to the other sampling sites. In the sediments, Mn was mainly in acid extractable form, Cu and Pb were mainly in reducible form, and As was mainly in residual form. The surface sediments at the sampling sites nearby the estuaries of the tributaries flowing through downtowns to the Reservoir had higher proportions of acid extractable and reducibles forms of the heavy metals, which would have definite potential toxic risk to aquatic organisms. Among the 7 heavy metals in the surface sediments, As showed the highest pollution degree, followed by Cu, Ni, Mn, Pb, and Zn, which were at moderate pollution degree, while Cr was at non-pollution degree, with relatively low potential ecological risk. Through the comparison of the sampling sites, it was observed that the surface sediments at the sites nearby the estuaries of Jinxi River and Hengxi River flowing through downtowns and heavy industrial parks to the Reservoir showed obviously higher heavy metals pollution degree and

Alimentation animale et organisation des filières : une comparaison pois protéagineux-lin oléagineux pour comprendre les facteurs freinant ou favorisant les cultures de diversification

Directory of Open Access Journals (Sweden)

Charrier François

2013-07-01

Full Text Available Le processus d’intensification des élevages, entamé dans les années 1970, s’est accompagné d’un développement croissant du recours aux aliments dits « composés », fabriqués à partir de différentes matières premières pour la plupart d’origine végétale. Les pratiques de la formulation des fabricants d’aliments composés, fondées sur une très forte substituabilité des matières premières, génèrent une concurrence très forte entre ces commodités. Dans un tel contexte de marché, la valorisation de certaines espèces repose sur des mécanismes permettant de contourner ou de s’affranchir de cette concurrence, mais dont l’efficacité reste fortement dépendante de l’organisation des acteurs au sein des filières. À partir de la comparaison des filières pois et lin, nous montrons ainsi qu’au-delà de la reconnaissance de propriétés nutritionnelles spécifiques pouvant valoriser différemment certaines espèces, la coordination des acteurs le long d’une filière est déterminante dans la diffusion des incitations économiques et informationnelles nécessaires à une orientation des agriculteurs en faveur d’espèces de diversification d’intérêt agro-écologique.

Verification and Validation of the Coastal Modeling System. Report 1: Summary Report

Science.gov (United States)

2011-12-01

Center, Coastal and Hydraulics Laboratory, Vicksburg, MS. Li, H., L. Lin, C. Lu and A.T. Shak . 2011 (In press). Evaluation of Breakwaters and...cirp.usace.army.mil/pubs/html/09-Li-Brown_TR-09-19.html, accessed 7 June 2011. Li, H., L. Lin, C. Lu andA.T. Shak . 2011 In press. Evaluation of Breakwaters and...TR-11-10 87 Reference: Li, H., L. Lin, C. Lu and A.T. Shak . 2011. Evaluation of Breakwaters and Sedimentation at Dana Point Harbor, CA

How Many Women Are Affected by or at Risk of Preeclampsia?

Science.gov (United States)

... pregnancy outcomes: Differences among 4 racial/ethnic groups. American Journal of Public Health, 95 (9), 1545–1551. Lin, H. C., Chen, S. F., Lin, H. C., & Chen, Y. H. (2010). Increased risk of adverse pregnancy outcomes ...

Murine bone marrow Lin⁻Sca⁻1⁺CD45⁻ very small embryonic-like (VSEL cells are heterogeneous population lacking Oct-4A expression.

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Krzysztof Szade

Full Text Available Murine very small embryonic-like (VSEL cells, defined by the Lin(-Sca-1(+CD45(- phenotype and small size, were described as pluripotent cells and proposed to be the most primitive hematopoietic precursors in adult bone marrow. Although their isolation and potential application rely entirely on flow cytometry, the immunophenotype of VSELs has not been extensively characterized. Our aim was to analyze the possible heterogeneity of Lin(-Sca(+CD45(- population and investigate the extent to which VSELs characteristics may overlap with that of hematopoietic stem cells (HSCs or endothelial progenitor cells (EPCs. The study evidenced that murine Lin(-Sca-1(+CD45(- population was heterogeneous in terms of c-Kit and KDR expression. Accordingly, the c-Kit(+KDR(-, c-Kit(-KDR(+, and c-Kit(-KDR(- subpopulations could be distinguished, while c-Kit(+KDR(+ events were very rare. The c-Kit(+KDR(- subset contained almost solely small cells, meeting the size criterion of VSELs, in contrast to relatively bigger c-Kit(-KDR(+ cells. The c-Kit(-KDR(-FSC(low subset was highly enriched in Annexin V-positive, apoptotic cells, hence omitted from further analysis. Importantly, using qRT-PCR, we evidenced lack of Oct-4A and Oct-4B mRNA expression either in whole adult murine bone marrow or in the sorted of Lin(-Sca-1(+CD45(-FSC(low population, even by single-cell qRT-PCR. We also found that the Lin(-Sca-1(+CD45(-c-Kit(+ subset did not exhibit hematopoietic potential in a single cell-derived colony in vitro assay, although it comprised the Sca-1(+c-Kit(+Lin(- (SKL CD34(-CD45(-CD105(+ cells, expressing particular HSC markers. Co-culture of Lin(-Sca-1(+CD45(-FSC(low with OP9 cells did not induce hematopoietic potential. Further investigation revealed that SKL CD45(-CD105(+ subset consisted of early apoptotic cells with fragmented chromatin, and could be contaminated with nuclei expelled from erythroblasts. Concluding, murine bone marrow Lin(-Sca-1(+CD45(-FSC(low cells are

Complementary feeding messages that target cultural barriers enhance both the use of lipid-based nutrient supplements and underlying feeding practices to improve infant diets in rural Zimbabwe.

Science.gov (United States)

Paul, Keriann H; Muti, Monica; Chasekwa, Bernard; Mbuya, Mduduzi N N; Madzima, Rufaro C; Humphrey, Jean H; Stoltzfus, Rebecca J

2012-04-01

Supplementation with lipid-based nutrient supplements (LiNS) is promoted as an approach to prevent child undernutrition and growth faltering. Previous LiNS studies have not tested the effects of improving the underlying diet prior to providing LiNS. Formative research was conducted in rural Zimbabwe to develop feeding messages to improve complementary feeding with and without LiNS. Two rounds of Trials of Improved Practices were conducted with mothers of infants aged 6-12 months to assess the feasibility of improving infant diets using (1) only locally available resources and (2) locally available resources plus 20 g of LiNS as Nutributter®/day. Common feeding problems were poor dietary diversity and low energy density. Popular improved practices were to process locally available foods so that infants could swallow them and add processed local foods to enrich porridges. Consumption of beans, fruits, green leafy vegetables, and peanut/seed butters increased after counselling (P < 0.05). Intakes of energy, protein, vitamin A, folate, calcium, iron and zinc from complementary foods increased significantly after counselling with or without the provision of Nutributter (P < 0.05). Intakes of fat, folate, iron, and zinc increased only (fat) or more so (folate, iron, and zinc) with the provision of Nutributter (P < 0.05). While provision of LiNS was crucial to ensure adequate intakes of iron and zinc, educational messages that were barrier-specific and delivered directly to mothers were crucial to improving the underlying diet. © 2010 Blackwell Publishing Ltd.

Classifying chemical mode of action using gene networks and machine learning: a case study with the herbicide linuron.

Science.gov (United States)

Ornostay, Anna; Cowie, Andrew M; Hindle, Matthew; Baker, Christopher J O; Martyniuk, Christopher J

2013-12-01

The herbicide linuron (LIN) is an endocrine disruptor with an anti-androgenic mode of action. The objectives of this study were to (1) improve knowledge of androgen and anti-androgen signaling in the teleostean ovary and to (2) assess the ability of gene networks and machine learning to classify LIN as an anti-androgen using transcriptomic data. Ovarian explants from vitellogenic fathead minnows (FHMs) were exposed to three concentrations of either 5α-dihydrotestosterone (DHT), flutamide (FLUT), or LIN for 12h. Ovaries exposed to DHT showed a significant increase in 17β-estradiol (E2) production while FLUT and LIN had no effect on E2. To improve understanding of androgen receptor signaling in the ovary, a reciprocal gene expression network was constructed for DHT and FLUT using pathway analysis and these data suggested that steroid metabolism, translation, and DNA replication are processes regulated through AR signaling in the ovary. Sub-network enrichment analysis revealed that FLUT and LIN shared more regulated gene networks in common compared to DHT. Using transcriptomic datasets from different fish species, machine learning algorithms classified LIN successfully with other anti-androgens. This study advances knowledge regarding molecular signaling cascades in the ovary that are responsive to androgens and anti-androgens and provides proof of concept that gene network analysis and machine learning can classify priority chemicals using experimental transcriptomic data collected from different fish species. © 2013.

A mutation in a coproporphyrinogen III oxidase gene confers growth inhibition, enhanced powdery mildew resistance and powdery mildew-induced cell death in Arabidopsis.

Science.gov (United States)

Guo, Chuan-yu; Wu, Guang-heng; Xing, Jin; Li, Wen-qi; Tang, Ding-zhong; Cui, Bai-ming

2013-05-01

A gene encoding a coproporphyrinogen III oxidase mediates disease resistance in plants by the salicylic acid pathway. A number of genes that regulate powdery mildew resistance have been identified in Arabidopsis, such as ENHANCED DISEASE RESISTANCE 1 to 3 (EDR1 to 3). To further study the molecular interactions between the powdery mildew pathogen and Arabidopsis, we isolated and characterized a mutant that exhibited enhanced resistance to powdery mildew. The mutant also showed dramatic powdery mildew-induced cell death as well as growth defects and early senescence in the absence of pathogens. We identified the affected gene by map-based cloning and found that the gene encodes a coproporphyrinogen III oxidase, a key enzyme in the tetrapyrrole biosynthesis pathway, previously known as LESION INITIATION 2 (LIN2). Therefore, we designated the mutant lin2-2. Further studies revealed that the lin2-2 mutant also displayed enhanced resistance to Hyaloperonospora arabidopsidis (H.a.) Noco2. Genetic analysis showed that the lin2-2-mediated disease resistance and spontaneous cell death were dependent on PHYTOALEXIN DEFICIENT 4 (PAD4), SALICYLIC ACID INDUCTION-DEFICIENT 2 (SID2), and NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1), which are all involved in salicylic acid signaling. Furthermore, the relative expression levels of defense-related genes were induced after powdery mildew infection in the lin2-2 mutant. These data indicated that LIN2 plays an important role in cell death control and defense responses in plants.

Aldehyde Dehydrogenase 1 and Raf Kinase Inhibitor Protein ...

African Journals Online (AJOL)

Xiang-Xiu Sun1, Qing-Shan Ma2, Tian-Feng Liu1, Ying Chen1, Yan Dong1 and. Lin-Lin Zhang1* ... cancer was developed and studied by histological examination. Immunohistochemical and .... (diaminobenzidine) Kit. Western blot analysis.

Carboxy-terminal truncation activates glp-1 protein to specify vulval fates in Caenorhabditis elegans.

Science.gov (United States)

Mango, S E; Maine, E M; Kimble, J

1991-08-29

The glp-1 and lin-12 genes encode homologous transmembrane proteins that may act as receptors for cell interactions during development. The glp-1 product is required for induction of germ-line proliferation and for embryogenesis. By contrast, lin-12 mediates somatic cell interactions, including those between the precursor cells that form the vulval hypodermis (VPCs). Here we analyse an unusual allele of glp-1, glp-1(q35), which displays a semidominant multivulva phenotype (Muv), as well as the typical recessive, loss-of-function Glp phenotypes (sterility and embryonic lethality). We find that the effects of glp-1(q35) on VPC development mimic those of dominant lin-12 mutations, even in the absence of lin-12 activity. The glp-1(q35) gene bears a nonsense mutation predicted to eliminate the 122 C-terminal amino acids, including a ProGluSerThr (PEST) sequence thought to destabilize proteins. We suggest that the carboxy terminus bears a negative regulatory domain which normally inactivates glp-1 in the VPCs. We propose that inappropriate glp-1(q35) activity can substitute for lin-12 to determine vulval fate, perhaps by driving the VPCs to proliferate.

Growth reduction of Listeria spp. caused by undefined industrial red smear cheese cultures and bacteriocin-producing Brevibacterium lines as evaluated in situ on soft cheese.

Science.gov (United States)

Eppert, I; Valdés-Stauber, N; Götz, H; Busse, M; Scherer, S

1997-01-01

The undefined microbial floras derived from the surface of ripe cheese which are used for the ripening of commercial red smear cheeses have a strong impact on the growth of Listeria spp. In some cases, these microbial consortia inhibit Listeria almost completely. From such undefined industrial cheese-ripening floras, linocin M18-producing (lin+) (N. Valdés-Stauber and S. Scherer, Appl. Environ. Microbiol. 60:3809-3814, 1994) and -nonproducing Brevibacterium linens strains were isolated and used as single-strain starter cultures on model red smear cheeses to evaluate their potential inhibitory effects on Listeria strains in situ. On cheeses ripened with lin+ strains, a growth reduction of L. ivanovii and L. monocytogenes of 1 to 2 log units was observed compared to cheeses ripened with lin strains. Linocin M18 activity was detected in cheeses ripened with lin+ strains but was not found in those ripened with lin strains. We suggest that production of linocin M18 contributes to the growth reduction of Listeria observed on model red smear cheeses but is unsufficient to explain the almost complete inhibition of Listeria caused by some undefined microbial floras derived from the surface of ripe cheeses. PMID:9406400

All Prime Contract Awards by State or Country, Place, and Contractor, FY 85. Part 15 (Altus, Oklahoma - Zelienople, Pennsylvania).

Science.gov (United States)

1985-01-01

Iso CM I m -4 0N 4 MNNNNNN"N-4-4 aN ION w O 40 UNNN liN 40.4 4 OR 60 4 14 .-4 -4 ..4 -4f -4 I-’ 1 0 0 0 0 0 0 0 0 0 -i aJC 0 4 1 4 i q--4-4--4 -- 4...4 azo azo ixN 0 .0 woo 000 0zo 01( C’) -I .4 0i~o x-0 0 4 N - ((0 04 03(0 ()I 1 -0 -4 0~00 x -0 0 04 " .(A 0 04 0.0 .4 0’( 4C I m i 41013 ə 3tr irn

Méthode intégro-variationnelle appliquée aux probèmes intérieur et extérieur avec surface libre de l'hydrodynamique linéaire Integro-Variational Method Applied to Indoor and Outdoor Problems with a Free Surface of Linear Hydrodinamics

Directory of Open Access Journals (Sweden)

Berhault C.

2006-11-01

Full Text Available Le but de cet article est de montrer que l'on peut appliquer une rintégro-variationnelle pour résoudre les problèmes intérieur (comportementliquide en milieu confiné et extérieur (diffraction-radiation de l'hydrodynamique linéaire. Une comparaison des résultats obtenus pour le problème intérieur dans Q les méthodes classiques : équation intégrale (singularités et formulation rationnelle (éléments finis, montre les avantages de cette méthode. The aim of this article is to show that an integro-variational method can be applied for solving interior (behavior of a liquid in a confined environment and exterior (diffraction-radiation problems of linear hydrodynamics. A comparison of the results obtained for the interior problem in IR2 with conventional methods, i.e. intégral équation (singularities and variational formulation (finite elements, shows the advantages of this method.

Theoretical studies of the global minima and polarizabilities of small lithium clusters

Energy Technology Data Exchange (ETDEWEB)

Hu, Hanshi; Zhao, Ya-Fan; Hammond, Jeffrey R.; Bylaska, Eric J.; Apra, Edoardo; van Dam, Hubertus JJ; Li, Jun; Govind, Niranjan; Kowalski, Karol

2016-01-16

Lithium clusters Lin (n=1-20) have been investigated with density functional theory (DFT) and coupled—cluster (CC) methods. The global-minimum structures are located via an improved basin---hopping algorithm and the lowest energy Lin isomers are confirmed with DFT geometry optimizations, CCSD(T) energy calculations, and by comparing simulated and experimental polarizabilities. The tetrahedral Li4 structure is found to be the basic building block of lithium clusters Lin (n=6-20). Simulated polarizabilities, including thermal effects at room temperature, are in good agreement with measured isotropic polarizabilities.

Three new species of Begonia (Begoniaceae from Limestone Hills in southwestern Sarawak, Borneo

Directory of Open Access Journals (Sweden)

Che-Wei Lin

2017-04-01

Full Text Available Most species of Begonia in Borneo, like those of other areas, are narrowly distributed and site-specific. In this study we report three new species of Begonia, namely B. felis C. W. Lin & C.-I Peng, B. kuchingensis C. W. Lin & C.-I Peng (sect. Petermannia and B. serianensis C. W. Lin & C.-I Peng (sect. Reichenheimia from the Padawan-Serian limestone hills in southwestern Sarawak. In addition to the taxonomic account, color plates, line drawings, a distribution map, and comparisons with phenetically similar species are provided to aid in identification.

Údržba mlecí linky v cementárenském podniku

OpenAIRE

Černý, Adam

2015-01-01

In this bachelor thesis titled Maintenance of grinding line in cement company is described the structure of maintenance and repair system in cement company. Thesis works with concrete company Českomoravský cement, a.s., Mokrá, part of international group HeidelbergCement Group, which is the biggest cement producer in Czech Republic. The introduction describes history and present of company Českomoravský cement, a.s., Mokrá, further author epmhesize detailed illustration of cement grinding lin...

Budget Studies of a Prefrontal Convective Rainband in Northern Taiwan Determined from TAMEX Data

Science.gov (United States)

1993-06-01

storm top accumulates less error in w calculation than an upward integration from the surface. Other Doppler studies, e.g., Chong and Testud (1983), Lin...contribute to the uncertainty of w is a result of the advection problem (Gal-Chen, 1982; Chong and Testud , 1983). Parsons et al (1983) employed an...Boulder, CO., 95-102. Chong, M., and J. Testud , 1983: Three-dimensional wind field analysis from dual-Doppler radar data. Part III: The boundary condition

The Seminested PCR Based Detection of Leishmania infantum Infection in Asymptomatic Dogs in a New Endemic Focus of Visceral Leishmaniasis in Iran

Directory of Open Access Journals (Sweden)

Y Rassi

2007-06-01

Full Text Available Visceral Leishmaniasis (Kala-azar is a serious health problem in some northern and south western parts of Iran. The incidence of kala-azar caused by Leishmania infantum has recently increased in Nourabad-Mamassani district of Fars Province, in the south of the country. This study was designed to determine the role of asymptomatic dogs as host reservoir of L. infantum in this new formed focus and detection of prevalence of infection near them. A total of 20 as¬ymptomatic stray and sheep dogs were randomly sampled. The Buffy coat layer of their peripheral blood was used for DNA extraction and PCR. A species specific seminested PCR was used for DNA amplification using LINR4, LIN17 and LIN19 primers. These primers amplified variable area of the minicircle kDNA of Leishmania parasites. Of the 20 sampled dogs checked for leishmanial kDNA, six (30% were found naturally infected. It is concluded that, dogs (Canis familiaris even if asympto¬matic, is considered as the domestic host reservoir of kala-azar in this endemic focus.

Tutorial de calcul poromécanique avec le logiciel Abaqus

OpenAIRE

Bonelli, S.

2011-01-01

/ Ce tutorial est destiné à apprendre à réaliser avec Abaqus, sous CAE, un calcul de poroélasticité linéaire. Il est écrit de telle sorte que quelqu'un n'ayant jamais utilisé Abaqus, mais ayant des connaissances de base en mécanique des milieux poreux d'une part, et en éléments-finis d'autre part, puisse réaliser un tel calcul en partant de zéro. Toutefois, il ne s'agit en aucun cas d'un tutorial sur Abaqus ui-même, ni sur la méthode des éléments-finis.

Two and three way spectrophotometric-assisted multivariate determination of linezolid in the presence of its alkaline and oxidative degradation products and application to pharmaceutical formulation

Science.gov (United States)

Hegazy, Maha Abd El-Monem; Eissa, Maya Shaaban; Abd El-Sattar, Osama Ibrahim; Abd El-Kawy, Mohammad

2014-07-01

Linezolid (LIN) is determined in the presence of its alkaline (ALK) and oxidative (OXD) degradation products without preliminary separation based on ultraviolet spectrophotometry using two-way chemometric methods; principal component regression (PCR) and partial least-squares (PLS), and three-way chemometric methods; parallel factor analysis (PARAFAC) and multi-way partial least squares (N-PLS). A training set of mixtures containing LIN, ALK and OXD; was prepared in the concentration ranges of 12-18, 2.4-3.6 and 1.2-1.8 μg mL-1, respectively according to a multilevel multifactor experimental design. The multivariate calibrations were obtained by measuring the zero-order absorbance from 220 to 320 nm using the training set. The validation of the multivariate methods was realized by analyzing their synthetic mixtures. The capabilities of the chemometric analysis methods for the analysis of real samples were evaluated by determination of LIN in its pharmaceutical preparation with satisfactory results. The accuracy of the methods, evaluated through the root mean square error of prediction (RMSEP), was 0.058, 0.026, 0.101 and 0.026 for LIN using PCR, PLS, PARAFAC and N-PLS, respectively. Protolytic equilibria of LIN and its degradation products were evaluated using the corresponding absorption spectra-pH data obtained with PARAFAC. The obtained pKa values of LIN, ALK and OXD are 5.70, 8.90 and 6.15, respectively. The results obtained were statistically compared to that of a reported HPLC method, and there was no significant difference between the proposed methods and the reported method regarding both accuracy and precision.

Optimization of linear consecutive-k-out-of-n system with a Birnbaum importance-based genetic algorithm

International Nuclear Information System (INIS)

Cai, Zhiqiang; Si, Shubin; Sun, Shudong; Li, Caitao

2016-01-01

The optimization of linear consecutive-k-out-of-n (Lin/Con/k/n) is to find an optimal component arrangement where n components are assigned to n positions to maximize the system reliability. With the interchangeability of components in practical systems, the optimization of Lin/Con/k/n systems is becoming widely applied in engineering practice, which is also a typical component assignment problem concerned by many researchers. This paper proposes a Birnbaum importance-based genetic algorithm (BIGA) to search the near global optimal solution for Lin/Con/k/n systems. First, the operation procedures and corresponding execution methods of BIGA are described in detail. Then, comprehensive simulation experiments are implemented on both small and large systems to evaluate the performance of the BIGA by comparing with the Birnbaum importance-based two-stage approach and Birnbaum importance-based genetic local search algorithm. Thirdly, further experiments are provided to discuss the applicability of BIGA for Lin/Con/k/n system with different k and n. Finally, the case study on oil transportation system is implemented to demonstrate the application of BIGA in the optimization of Lin/Con/k/n system. - Highlights: • BIGA integrates BI and GA to solve the Lin/Con/k/n systems optimization problems. • The experiment results show that the BIGA performs well in most conditions. • Suggestions are given for the application of BIGA and BITA with different k and n. • The application procedure of BIGA is demonstrated by the oil transportation system.

acn-1, a C. elegans homologue of ACE, genetically interacts with the let-7 microRNA and other heterochronic genes.

Science.gov (United States)

Metheetrairut, Chanatip; Ahuja, Yuri; Slack, Frank J

2017-10-02

The heterochronic pathway in C. elegans controls the relative timing of cell fate decisions during post-embryonic development. It includes a network of microRNAs (miRNAs), such as let-7, and protein-coding genes, such as the stemness factors, LIN-28 and LIN-41. Here we identified the acn-1 gene, a homologue of mammalian angiotensin-converting enzyme (ACE), as a new suppressor of the stem cell developmental defects of let-7 mutants. Since acn-1 null mutants die during early larval development, we used RNAi to characterize the role of acn-1 in C. elegans seam cell development, and determined its interaction with heterochronic factors, including let-7 and its downstream interactors - lin-41, hbl-1, and apl-1. We demonstrate that although RNAi knockdown of acn-1 is insufficient to cause heterochronic defects on its own, loss of acn-1 suppresses the retarded phenotypes of let-7 mutants and enhances the precocious phenotypes of hbl-1, though not lin-41, mutants. Conversely, the pattern of acn-1 expression, which oscillates during larval development, is disrupted by lin-41 mutants but not by hbl-1 mutants. Finally, we show that acn-1(RNAi) enhances the let-7-suppressing phenotypes caused by loss of apl-1, a homologue of the Alzheimer's disease-causing amyloid precursor protein (APP), while significantly disrupting the expression of apl-1 during the L4 larval stage. In conclusion, acn-1 interacts with heterochronic genes and appears to function downstream of let-7 and its target genes, including lin-41 and apl-1.

314 A Study of Secondary School Students' Academic Performance ...

African Journals Online (AJOL)

User

Students' Academic Performance in English Language and Mathematics ... passed at credit level by secondary schools students in public examinations. A credit level ..... ls O n lin e: www.a jo l.in fo. T ab le 1. : P ercen tage of p asses and failu.

Linéarité et développement d'un système technique Linearity and Development of a Technical System

Directory of Open Access Journals (Sweden)

Dautray R.

2006-11-01

Full Text Available Il est examiné comment la structurede la modélisation d'un phénomène ou d'un processus ou du fonctionnement d'un appareil joue un rôle déterminant dans le plan des expériences destinées à obtenir les connaissances nécessaires à son étude et à sa réalisation. Dans le cas d'une structure linéaire , il est montré, sur l'exemple de l'énergie nucléaire, que ces expériences peuvent être menées sur des appareils distincts, les résultats de l'une servant à déterminer les entrées de l'autre. Ce découplagepermet de mener en parallèle un très grand programme d'expériences, et donc de construire relativement rapidement ces nouvelles technologies. C'est en effet le cas de l'énergie nucléaire de fission : - Le phénomène de fission a été découvert en 1938. - La démonstration de la faisabilité physique de la réaction en chaîne a été démontrée en 1942. - Les premiers réacteurs nucléaires de puissance ont fonctionné à Hanford en 1945. - Et dès les années cinquante, des réacteurs nucléaires actionnaient des sous-marins et des prototypes de chaudières nucléaires pour centrales (Shippingport et Calder Hall. This article examines how the structureof the modeling of a phenomenon or process or the operating of a piece of equipment plays a determinant role in the planning of experiments for the purpose of obtaining the understanding required to analyze it and create it. For a linear structure , the example of nuclear energy is taken to show that these experiments can be performed with distinct pieces of equipment, with the results of one being used to determine the input for the other. This uncouplingenables a very widespread experimental project to be carried out in parallel, so that these new technologies can be built up relatively quickly. This is effectively true for nuclear energy from fission. - The phenomenon of fission was discovered in 1938. - The physical feasibility of the chain reaction was demonstrated

Feminist Encounters with Evolutionary Psychology

OpenAIRE

O'Neill, Rachel

2016-01-01

This Section of Australian Feminist Studies is the product of an event that took place at King’s College London in January 2015, hosted as part of the UK-based ‘Critical Sexology’ seminar series. Participants at this event – feminist scholars working across the fields of lin- guistics, cultural studies, sociology, and psychology – were invited to reflect on their encounters with evolutionary psychology (EP). As the event organiser, I was interested to prompt a discussion about how EP shapes t...

Electrical power line and pole removal radiological survey completion report: Revision 2

International Nuclear Information System (INIS)

1989-02-01

Each electric power pole and all wire, cross members, and attached hardware were radiologically surveyed and removed. The survey procedures did not conform in every respect with the planned procedures because the actual work differed from the removal plan. The survey showed that all contamination was fixed. Certain poles that were suspected of being contaminated with Th-230 were cut off one foot above ground level and left on site. Each truck load of materials was checked at the access point to ensure it met release criteria. Wood samples were taken from all the poles at Building 403 and 5% of all the rest and analyzed for U-238 and Th-232. Only U-238 was detected. 1903 bundles of wire (95,150 lin ft) were released, and 76 bundles (1520 lin ft) were found to be contaminated. 7163 lin ft of power pole material were released, and 1484 lin ft were contaminated. A comprehensive quality measure assurance/quality control program was applied to this work

Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma

Science.gov (United States)

Powers, John T; Tsanov, Kaloyan M; Pearson, Daniel S; Roels, Frederik; Spina, Catherine S; Ebright, Richard; Seligson, Marc; de Soysa, Yvanka; Cahan, Patrick; Theiβen, Jessica; Tu, Ho-Chou; Han, Areum; Kurek, Kyle C; LaPier, Grace S; Osborne, Jihan K; Ross, Samantha J; Cesana, Marcella; Collins, James J; Berthold, Frank; Daley, George Q

2016-01-01

Poor prognosis in neuroblastoma is associated with genetic amplification of MYCN. MYCN is itself a target of let-7, a tumor suppressor family of microRNAs implicated in numerous cancers. LIN28B, an inhibitor of let-7 biogenesis, is overexpressed in neuroblastoma and has been reported to regulate MYCN. However, here we show that LIN28B is dispensable in MYCN-amplified neuroblastoma cell lines, despite de-repression of let-7. We further demonstrate that MYCN mRNA levels in amplified disease are exceptionally high and sufficient to sponge let-7, which reconciles the dispensability of LIN28B. We found that genetic loss of let-7 is common in neuroblastoma, inversely associated with MYCN-amplification, and independently associated with poor outcomes, providing a rationale for chromosomal loss patterns in neuroblastoma. We propose that let-7 disruption by LIN28B, MYCN sponging, or genetic loss is a unifying mechanism of neuroblastoma pathogenesis with broad implications for cancer pathogenesis. PMID:27383785

Biochemical diversity of fatty acid composition in flax from VIR genetic collection and effect of environment on its development

Directory of Open Access Journals (Sweden)

Elizaveta A Porokhovinova

2016-03-01

Full Text Available Background. In connection with climate change vary known patterns of environmental influences on the ratio of fatty acids(FA in oil. Therefore,relevant data of modern geography test. Materials and methods. In work 24 lines and 3 commercial varieties of flax including 3 low linolenic (LL accessions, grown in the Leningrad and Samara regions were used. FA composition was evaluated by gas chromatography for the ratio of palmitic (PAL, stearic (STE, oleic (OLE, linoleic (ω6,LIO, linolenic (ω3, LIN acids, ω6/ω3 and iodine number of the oil (IOD. Results. The strongest differences are due to the level of LIN. It is lower in LL and gc-119 from India and higher in 3 lines carrying the gene s1 (deranged anthocyanin biosynthesis. In gc-119, contrast to LL, LIN decrease increase of OLE, instead of LIO. In lines with the gene s1 LIN increase due to the OLE reduction. Contrary to earlier publications the seeds of northern reproduction have more PAL, OLE, less LIN, IOD. 2F ANOVA revealed significant effect of genotype and reproductions place on PAL, OLE, LIN, IOD. LIO is affected only by genotype. Independence of ω6/ω3 is explained by strong abnormity of distribution due to LL. In high linolenic (HL accessions group both factors influenced all characters except STE. Kruskal-Wallis H test (non-parametric 1F ANOVA analogue show significant effect of genotype and place of reproductions on ω6/ω3. It reveals the impact of the reproduction place on LIN,no significant effect of genotype on OLE and IOD, which in the case of 1F ANOVA were significant. For characters of HL with normal distribution, comparing of both tests showed that in case of 0,01 0,10 they are identical. Conclusion. In our studies the geographical effect is less important than the weather in the year of growing. For abnormal distribution it is desirable to use both statistics and carefully make conclusions about the significance of differences in borderline probabilities.

Single-tube linear DNA amplification for genome-wide studies using a few thousand cells

NARCIS (Netherlands)

Shankaranarayanan, P.; Mendoza-Parra, M.A.; Gool, van W.; Trindade, L.M.; Gronemeyer, H.

2012-01-01

Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled

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African Journals Online (AJOL)

Dr Olaleye

Histological assessment of the gastric mucosa and submucosa revealed infiltration by inflammatory cells in normal ... Gastric ulcer, also known as peptic ulcer, is a localized area of erosion in ..... and water retention by interfering with the prostaglandin induced ... Clark JD, Lin LL, Kriz RW, Ramesha CS, Sultzman LA,. Lin AY ...

75 FR 4509 - Uniform Criteria for State Observational Surveys of Seat Belt Use

Science.gov (United States)

2010-01-28

... statistical issues: Ms. Chou-Lin Chen, Chief, Mathematical Analysis Division, National Center for Statistics..., Washington, DC 20590. Telephone number: 202-366-1048; E- mail: Chou-Lin.Chen@dot.gov . For legal issues: Ms..., and reduce burden. E. Paperwork Reduction Act Under the Paperwork Reduction Act of 1995, a person is...

First SN Discoveries from the Dark Energy Survey

Science.gov (United States)

Abbott, T.; Abdalla, F.; Achitouv, I.; Ahn, E.; Aldering, G.; Allam, S.; Alonso, D.; Amara, A.; Annis, J.; Antonik, M.; Aragon-Salamanca, A.; Armstrong, R.; Ashall, C.; Asorey, J.; Bacon, D.; Balbinot, E.; Banerji, M.; Barbary, K.; Barkhouse, W.; Baruah, L.; Bauer, A.; Bechtol, K.; Becker, M.; Bender, R.; Benoist, C.; Benoit-Levy, A.; Bernardi, M.; Bernstein, G.; Bernstein, J. P.; Bernstein, R.; Bertin, E.; Beynon, E.; Bhattacharya, S.; Biesiadzinski, T.; Biswas, R.; Blake, C.; Bloom, J. S.; Bocquet, S.; Brandt, C.; Bridle, S.; Brooks, D.; Brown, P. J.; Brunner, R.; Buckley-Geer, E.; Burke, D.; Burkert, A.; Busha, M.; Campa, J.; Campbell, H.; Cane, R.; Capozzi, D.; Carlstrom, J.; Carnero Rosell, A.; Carollo, M.; Carrasco-Kind, M.; Carretero, J.; Carter, M.; Casas, R.; Castander, F. J.; Chen, Y.; Chiu, I.; Chue, C.; Clampitt, J.; Clerkin, L.; Cohn, J.; Colless, M.; Copeland, E.; Covarrubias, R. A.; Crittenden, R.; Crocce, M.; Cunha, C.; da Costa, L.; d'Andrea, C.; Das, S.; Das, R.; Davis, T. M.; Deb, S.; DePoy, D.; Derylo, G.; Desai, S.; de Simoni, F.; Devlin, M.; Diehl, H. T.; Dietrich, J.; Dodelson, S.; Doel, P.; Dolag, K.; Efstathiou, G.; Eifler, T.; Erickson, B.; Eriksen, M.; Estrada, J.; Etherington, J.; Evrard, A.; Farrens, S.; Fausti Neto, A.; Fernandez, E.; Ferreira, P. C.; Finley, D.; Fischer, J. A.; Flaugher, B.; Fosalba, P.; Frieman, J.; Furlanetto, C.; Garcia-Bellido, J.; Gaztanaga, E.; Gelman, M.; Gerdes, D.; Giannantonio, T.; Gilhool, S.; Gill, M.; Gladders, M.; Gladney, L.; Glazebrook, K.; Gray, M.; Gruen, D.; Gruendl, R.; Gupta, R.; Gutierrez, G.; Habib, S.; Hall, E.; Hansen, S.; Hao, J.; Heitmann, K.; Helsby, J.; Henderson, R.; Hennig, C.; High, W.; Hirsch, M.; Hoffmann, K.; Holhjem, K.; Honscheid, K.; Host, O.; Hoyle, B.; Hu, W.; Huff, E.; Huterer, D.; Jain, B.; James, D.; Jarvis, M.; Jarvis, M. J.; Jeltema, T.; Johnson, M.; Jouvel, S.; Kacprzak, T.; Karliner, I.; Katsaros, J.; Kent, S.; Kessler, R.; Kim, A.; Kim-Vy, T.; King, L.; Kirk, D.; Kochanek, C.; Kopp, M.; Koppenhoefer, J.; Kovacs, E.; Krause, E.; Kravtsov, A.; Kron, R.; Kuehn, K.; Kuemmel, M.; Kuhlmann, S.; Kunder, A.; Kuropatkin, N.; Kwan, J.; Lahav, O.; Leistedt, B.; Levi, M.; Lewis, P.; Liddle, A.; Lidman, C.; Lilly, S.; Lin, H.; Liu, J.; Lopez-Arenillas, C.; Lorenzon, W.; LoVerde, M.; Ma, Z.; Maartens, R.; Maccrann, N.; Macri, L.; Maia, M.; Makler, M.; Manera, M.; Maraston, C.; March, M.; Markovic, K.; Marriner, J.; Marshall, J.; Marshall, S.; Martini, P.; Marti Sanahuja, P.; Mayers, J.; McKay, T.; McMahon, R.; Melchior, P.; Merritt, K. W.; Merson, A.; Miller, C.; Miquel, R.; Mohr, J.; Moore, T.; Mortonson, M.; Mosher, J.; Mould, J.; Mukherjee, P.; Neilsen, E.; Ngeow, C.; Nichol, R.; Nidever, D.; Nord, B.; Nugent, P.; Ogando, R.; Old, L.; Olsen, J.; Ostrovski, F.; Paech, K.; Papadopoulos, A.; Papovich, C.; Patton, K.; Peacock, J.; Pellegrini, P. S. S.; Peoples, J.; Percival, W.; Perlmutter, S.; Petravick, D.; Plazas, A.; Ponce, R.; Poole, G.; Pope, A.; Refregier, A.; Reyes, R.; Ricker, P.; Roe, N.; Romer, K.; Roodman, A.; Rooney, P.; Ross, A.; Rowe, B.; Rozo, E.; Rykoff, E.; Sabiu, C.; Saglia, R.; Sako, M.; Sanchez, A.; Sanchez, C.; Sanchez, E.; Sanchez, J.; Santiago, B.; Saro, A.; Scarpine, V.; Schindler, R.; Schmidt, B. P.; Schmitt, R. L.; Schubnell, M.; Seitz, S.; Senger, R.; Sevilla, I.; Sharp, R.; Sheldon, E.; Sheth, R.; Smith, R. C.; Smith, M.; Snigula, J.; Soares-Santos, M.; Sobreira, F.; Song, J.; Soumagnac, M.; Spinka, H.; Stebbins, A.; Stoughton, C.; Suchyta, E.; Suhada, R.; Sullivan, M.; Sun, F.; Suntzeff, N.; Sutherland, W.; Swanson, M. E. C.; Sypniewski, A. J.; Szepietowski, R.; Talaga, R.; Tarle, G.; Tarrant, E.; Balan, S. Thaithara; Thaler, J.; Thomas, D.; Thomas, R. C.; Tucker, D.; Uddin, S. A.; Ural, S.; Vikram, V.; Voigt, L.; Walker, A. R.; Walker, T.; Wechsler, R.; Weinberg, D.; Weller, J.; Wester, W.; Wetzstein, M.; White, M.; Wilcox, H.; Wilman, D.; Yanny, B.; Young, J.; Zablocki, A.; Zenteno, A.; Zhang, Y.; Zuntz, J.

2012-12-01

The Dark Energy Survey (DES) report the discovery of the first set of supernovae (SN) from the project. Images were observed as part of the DES Science Verification phase using the newly-installed 570-Megapixel Dark Energy Camera on the CTIO Blanco 4-m telescope by observers J. Annis, E. Buckley-Geer, and H. Lin. SN observations are planned throughout the observing campaign on a regular cadence of 4-6 days in each of the ten 3-deg2 fields in the DES griz filters.

Photonic-Networks-on-Chip for High Performance Radiation Survivable Multi-Core Processor Systems

Science.gov (United States)

2013-12-01

Loss Spectra” Proceedings of SPIE 8255, (2012) and in a journal publication: M. T. Crowley, D. Murrell, N. Patel, M. Breivik , C.-Y. Lin, Y. Li, B.-O...Crowley, D. Murrell, N. Patel, M. Breivik , C.-Y. Lin, Y. Li, B.-O. Fimland and L. F. Lester, "Analytical Modeling of the Temperature Performance of

Water SA - Vol 40, No 2 (2014)

African Journals Online (AJOL)

The development of GIS-PMWIN and its application for mine-water modelling in the Far West Rand, South Africa · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT · DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. H Lin, L Lin, 313-322. http://dx.doi.org/10.4314/wsa.v40i2.13 ...

Deficiency of a membrane skeletal protein, 4.1G, results in myelin abnormalities in the peripheral nervous system.

Science.gov (United States)

Saitoh, Yurika; Ohno, Nobuhiko; Yamauchi, Junji; Sakamoto, Takeharu; Terada, Nobuo

2017-12-01

We previously demonstrated that a membrane skeletal molecular complex, 4.1G-membrane palmitoylated protein 6 (MPP6)-cell adhesion molecule 4, is incorporated in Schwann cells in the peripheral nervous system (PNS). In this study, we evaluated motor activity and myelin ultrastructures in 4.1G-deficient (-/-) mice. When suspended by the tail, aged 4.1G -/- mice displayed spastic leg extension, especially after overwork. Motor-conduction velocity in 4.1G -/- mice was slower than that in wild-type mice. Using electron microscopy, 4.1G -/- mice exhibited myelin abnormalities: myelin was thicker in internodes, and attachment of myelin tips was distorted in some paranodes. In addition, we found a novel function of 4.1G for sorting a scaffold protein, Lin7, due to disappearance of the immunolocalization and reduction of the production of Lin7c and Lin7a in 4.1G -/- sciatic nerves, as well as the interaction of MPP6 and Lin7 with immunoprecipitation. Thus, we herein propose 4.1G functions as a signal for proper formation of myelin in PNS.

Unbiased Combinatorial Genomic Approaches to Identify Alternative Therapeutic Targets within the TSC Signaling Network

Science.gov (United States)

2015-09-01

endonuclease. Nat Biotechnol. 2013;31(3):230-2. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, et al. Multiplex genome engineering using CRISPR/Cas systems...Res 42, e89 (2014)10.1093/nar/gku289). 13. L. Cong, F. A. Ran, D. Cox, S. Lin, R. Barretto, N. Habib , P. D. Hsu, X. Wu, W. Jiang, L. A. Marraffini

Identification of Plasmodiophora brassicae Wor. isolate suppressing clubroot resistance in ‘Kilaxy’ F1 white cabbage

Directory of Open Access Journals (Sweden)

Wesołowska Maria

2014-06-01

Full Text Available Celem badań była ocena patogeniczności izolatów Plasmodiophora brassicae Wor. pobranych z porażonych kiłą roślin kapusty głowiastej białej pochodzących z różnych rejonów Polski. Oceniano wirulencję siedmiu izolatów tego patogena. Do określenia wirulencji izolatów P. brassicae użyte zostały obiekty wzorcowe, były to materiały hodowlane kapusty głowiastej białej odporne i podatne na kiłę. Nasiona kapusty inokulowano poprzez moczenie ich w zawiesinie zarodników przetrwalnikowych patogena. Ocenę podatności roślin na kiłę prowadzono w oparciu o 9-cio stopniową skalę porażenia korzeni roślin w stadium ośmiotygodniowej rozsady i podczas jesiennego zbioru roślin na polu zainfekowanym P. brassicae. Izolaty pochodzące z Pobiednika i Goźlic były bardziej wirulentne. Izolat P z Pobiednika spowodował porażenie roślin odmiany ‘Kilaxy’ F1 co oznaczało przełamanie cechującej tę odmianę odporności na kiłę. Odmiana ta w stosunku do pozostałych izolatów potwierdziła swoją odporność. Wrażliwa linia HTM charakteryzowała się najwyższym stopniem porażenia. Odnotowano różny poziom wrażliwości na zastosowane izolaty pomiędzy liniami pochodzącymi z mających geny odporności odmian ‘Oregon123’ i ‘Badger Shipper’. Mniej podatna na zastosowane izolaty była linia ‘Oregon123’. Obserwowano wpływ zastosowanych do inokulacji nasion izolatów na reakcję na patogena dojrzałych roślin kapusty na polu kiłowym

Pancreatic stellate cells enhance stem cell-like phenotypes in pancreatic cancer cells

International Nuclear Information System (INIS)

Hamada, Shin; Masamune, Atsushi; Takikawa, Tetsuya; Suzuki, Noriaki; Kikuta, Kazuhiro; Hirota, Morihisa; Hamada, Hirofumi; Kobune, Masayoshi; Satoh, Kennichi; Shimosegawa, Tooru

2012-01-01

Highlights: ► Pancreatic stellate cells (PSCs) promote the progression of pancreatic cancer. ► Pancreatic cancer cells co-cultured with PSCs showed enhanced spheroid formation. ► Expression of stem cell-related genes ABCG2, Nestin and LIN28 was increased. ► Co-injection of PSCs enhanced tumorigenicity of pancreatic cancer cells in vivo. ► This study suggested a novel role of PSCs as a part of the cancer stem cell niche. -- Abstract: The interaction between pancreatic cancer cells and pancreatic stellate cells (PSCs), a major profibrogenic cell type in the pancreas, is receiving increasing attention. There is accumulating evidence that PSCs promote the progression of pancreatic cancer by increasing cancer cell proliferation and invasion as well as by protecting them from radiation- and gemcitabine-induced apoptosis. Recent studies have identified that a portion of cancer cells, called “cancer stem cells”, within the entire cancer tissue harbor highly tumorigenic and chemo-resistant phenotypes, which lead to the recurrence after surgery or re-growth of the tumor. The mechanisms that maintain the “stemness” of these cells remain largely unknown. We hypothesized that PSCs might enhance the cancer stem cell-like phenotypes in pancreatic cancer cells. Indirect co-culture of pancreatic cancer cells with PSCs enhanced the spheroid-forming ability of cancer cells and induced the expression of cancer stem cell-related genes ABCG2, Nestin and LIN28. In addition, co-injection of PSCs enhanced tumorigenicity of pancreatic cancer cells in vivo. These results suggested a novel role of PSCs as a part of the cancer stem cell niche.

Controlled parts management

International Nuclear Information System (INIS)

Boyle, C.M.; Hidalgo, S.P.; Martinez, B.J.

2004-01-01

The Controlled Parts Management (CPM) system is based on industry standard practices for managing inventory. CPM is designed to record the movement of any type of inventory in a defined region referred to as an Account or SubAccount. The system is used to track the receiving, processing, storing and transfer of inventory parts. CPM provides information on parts, quantity and the exact location of the inventory. CPM is a barcode-based-part tracking system currently used to track controlled parts that are used in the R and D and testing of weapons; this tracking helps maintain the part pedigree that is required for certification of a weapon or weapon test. CPM includes bar code data collection software programmed into portable bar code readers for automating physical inventory services and remote transaction capture. CPM interfaces to other Engineering systems and supports a 'material content' of a weapons test through the test Bill of Materials and assignment of a unique inventory part in CPM. Additional functionality includes the ability to group or join parts, logically or physically and temporary or permanent, to represent discrete parts, containers, subassemblies and assemblies, and groupings.

Effects of anti-androgens cyproterone acetate, linuron, vinclozolin, and p,p'-DDE on the reproductive organs of the copepod Acartia tonsa.

Science.gov (United States)

Watermann, Burkard T; Albanis, Triantafyllos A; Galassi, Silvana; Gnass, Katarina; Kusk, Kresten O; Sakkas, Vasilios A; Wollenberger, Leah

2016-11-09

The study was performed to detect the effects of anti-androgenic compounds on the reproduction. In this paper alterations observed in the marine calanoid copepod Acartia tonsa exposed to environmental concentrations of cyproterone acetate (CPA), linuron (LIN), vinclozolin (VIN), and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE) for 21 days covering a full life cycle are described. Histological alterations were studied with a focus on reproductive organs, gonad and accessory sexual glands. Exposure to ≥1.2 µg L(-1) CPA caused degeneration of spermatocytes and deformation of the spermatophore in males. In a single male exposed to 33 µg L(-1) CPA, an ovotestis was observed. In CPA exposed females, enhancement of oogenesis, increase in apoptosis and a decrease in proliferation occurred. Exposure of males to ≥12 µg L(-1) LIN caused degenerative effects in spermatogonia, spermatocytes and spermatids, and at 4.7 µg L(-1) LIN, the spermatophore wall displayed an irregular formation. In LIN exposed females, no such structural alterations were found; however, the proliferation index was reduced at 29 µg L(-1) LIN. At an exposure concentration of ≥100 µg L(-1) VIN, distinct areas in male gonad were stimulated, whereas others displayed a disturbed spermatogenesis and a deformed spermatophore wall. In VIN exposed female A. tonsa, no effects were observed. Male A. tonsa exposed to p,p'-DDE displayed an impairment of spermatogenesis in all stages with increased degrees of apoptosis. In p,p'-DDE-exposed females, a statistical significant increase of the proliferation index and an intensification of oogenesis were observed at 0.0088 µg L(-1).

Protecting Networks Via Automated Defense of Cyber Systems

Science.gov (United States)

2016-09-01

gray-hats/. 61 Hu, Youfan, Yan Zhang, Chen Xu, Long Lin, Robert L. Snyder, and Zhong Lin Wang. “Self-Powered System with Wireless Data Transmission ...wires will almost completely cease to exist, replaced by mobile nanodevices, which are tiny computers measured in nanometers with integrated wireless ... energy companies, was hypothesized to cost $10,000. Raiu went on to opine, “The cost of entry for [advanced persistent threats] is decreasing …. We’re

Estimation of genetic parameters in three commercial silk-worm ...

African Journals Online (AJOL)

Jane

2011-10-10

Oct 10, 2011 ... Gener. Lin.×Gene. S.S.. 0.0005. 0.0016. 0.0550. 0.0303. 0.0896. 0.2441. 0.1202. 0.7733. M.S.. 0.0002. 0.0004. 0.0275. 0.0151. 0.0224. 0.1220. 0.0601. 0.1933. F value. 1.790. 2.790. 0.930. 0.510. 0.760. 2.540. 1.250. 4.010. Gene., generation; lin, line. Table 6. Comparison of change in breeding value in ...

SELES

DEFF Research Database (Denmark)

Garcia-Zamora, Claudia; Rodriguez-Henriquez, Francisco; Ortiz-Arroyo, Daniel

2005-01-01

In this paper we present an improved e-voting protocol for online elections based on the scheme proposed by Lin--Hwang--Chang. We show that Lin--Hwang--Chang's scheme has two important weaknesses. We describe in detail our findings and propose a new scheme to overcome these weaknesses effectively....... To test our protocol, we implemented a distributed e-voting system. Finally we evaluate and compare our protocol with other previously proposed schemes....

Piezoelectric Nanogenerators for Self-Powered Nanosystems and Nanosensors

Science.gov (United States)

2013-05-15

researchers 1. Sangmin Lee, Sung-Hwan Bae, Long Lin, Seunghyun Ahn, Chan Park, Seung Nam Cha, Young Jun Park, Hyuk Chang, Sang-Woo Kim and...Sohn, Seung Nam Cha, Dukhyun Choi * Zhong Lin Wang * Sang-Woo Kim * “ P-Type Polymer–Hybridized High-Performance Piezoelectric Nanogenerators”, Nano...Yoon Lee, † Sang-Woo Kim , *,$ Jae-Young Choi, *,† Jong Min Kim , † and Zhong Lin Wang *, “Flexible Hybrid Multi-Type Energy Scavenger”, Energy

Molecular Signatures and Diagnostic Biomarkers of Cumulative, Blast-Graded Mild TBI

Science.gov (United States)

2013-10-01

ischemic stroke. Neurol. Res. 30, 783–793. 35. Wang, H.C., Lin, W.C., Lin, Y.J., Rau, C.S., Lee, T.H., Chang, W.N., Tsai, N.W., Cheng, B.C., Kung , C.T., and...J.K., Shen, C.A., Yin, H.N., Zhou, X.F., Lu, W., Hu, Q.G., Chi, Y.F., Ma, L., 22  Deng, H.P., Zhang, X.B., Sun, T.J., and Han , Y.F. (2010). [Early

RLE (Research Laboratory of Electronics) Progress Report Number 130

Science.gov (United States)

1988-07-01

Ippen, James G. Fujimoto, Wei-Zhu Lin, Beat Zysset, Robert W. Schoenlein, 4 Michael J. Lagasse The investigation of transient carrier dynamics in GaAs...G. Fujimoto, Wei-Zhu Lin, Reginald Birngruber, Beat Zysset, Robert W. Schoenleln Working in collaboration with researchers at the Massachusetts Eye... Binaural Hearing National Institutes of Health (Grant 5 RO 1 NS 10916) H. Steven Colburn, Nathaniel 1. Durlach, Patrick M. Zurek 17 Brooklyn College14 141

The part task of the part-spacing paradigm is not a pure measurement of part-based information of faces.

Directory of Open Access Journals (Sweden)

Qi Zhu

Full Text Available BACKGROUND: Faces are arguably one of the most important object categories encountered by human observers, yet they present one of the most difficult challenges to both the human and artificial visual systems. A variety of experimental paradigms have been developed to study how faces are represented and recognized, among which is the part-spacing paradigm. This paradigm is presumed to characterize the processing of both the featural and configural information of faces, and it has become increasingly popular for testing hypotheses on face specificity and in the diagnosis of face perception in cognitive disorders. METHODOLOGY/PRINCIPAL FINDINGS: In two experiments we questioned the validity of the part task of this paradigm by showing that, in this task, measuring pure information about face parts is confounded by the effect of face configuration on the perception of those parts. First, we eliminated or reduced contributions from face configuration by either rearranging face parts into a non-face configuration or by removing the low spatial frequencies of face images. We found that face parts were no longer sensitive to inversion, suggesting that the previously reported inversion effect observed in the part task was due in fact to the presence of face configuration. Second, self-reported prosopagnosic patients who were selectively impaired in the holistic processing of faces failed to detect part changes when face configurations were presented. When face configurations were scrambled, however, their performance was as good as that of normal controls. CONCLUSIONS/SIGNIFICANCE: In sum, consistent evidence from testing both normal and prosopagnosic subjects suggests the part task of the part-spacing paradigm is not an appropriate task for either measuring how face parts alone are processed or for providing a valid contrast to the spacing task. Therefore, conclusions from previous studies using the part-spacing paradigm may need re-evaluation with

A Design Framework for Predictive Engine Control Un cadre de conception pour la commande prédictive de moteurs

Directory of Open Access Journals (Sweden)

Wang X.

2011-09-01

Full Text Available Model Predictive Control (MPC has been proposed several times for automotive control, with promising results, mostly based on a linear MPC approach. However, as most automotive systems are nonlinear, Nonlinear MPC (NMPC would be an interesting option. Unfortunately, an optimal control design with a generic nonlinear model usually leads to a complex, non convex problem. Against this background, this paper presents two different schemes to take into account the system nonlinearity in the control design. First, a multi-linear MPC method is shown based on the segmentation of the system and then a control system design based on a nonlinear system identification using a quasi Linear Parameter Varying (LPV structure is proposed, which is then used in a NMPC design framework. This paper presents the approaches and the application to a well studied system, the air path of a Diesel engine. La commande prédictive par modèle (Model Predictive Control; MPC a été proposée plusieurs fois dans l’automatique pour l’automobile, avec des résultats prometteurs, principalement à partir d’une approche MPC linéaire. Toutefois, comme la plupart des systèmes automobiles sont non linéaires, la commande prédictive non linéaire (Nonlinear MPC; NMPC pourrait représenter une option intéressante. Malheureusement, la conception d’une commande optimale à partir d’un modèle non linéaire générique conduit généralement à un problème non convexe complexe. Dans ce contexte, cet article présente deux schémas différents pour prendre en compte la non linéarité du système en vue de la conception de la commande. En premier lieu, une méthode MPC multilinéaire est présentée sur la base d’une segmentation du système et, en second lieu, une conception de système de commande basée sur une identification de système non linéaire utilisant une structure quasi linéaire à paramètres variants (Linear Parameter Varying; LPV est proposée; celle

Étude numérique du couplage thermohydromécanique dans les roches. Influence des termes de couplage non linéaires pour un matériau isotrope linéaire Numerical Analysis of a Thermohydromechanical Coupling in Rocks. Influence of Nonlinear Coupling Terms on a Linear Isotropic Material

Directory of Open Access Journals (Sweden)

Henry J. P.

2006-11-01

Full Text Available Nous présentons dans ce travail une étude numérique basée sur la méthode des éléments finis, du comportement thermoporoélastique de certaines roches. Les trois effets de couplage : déformabilité de la roche, pression interstitielle et température sont pris en compte simultanément dans la résolution numérique. Une application simple sur un puits pétrolier en conditions axisymétriques est finalement présentée afin de dégager en particulier l'influence du terme de couplage convectif non linéaire, obtenu dans l'équation de diffusivité thermique, sur l'évolution de la température et de la pression interstitielle autour du forage. This article describes a finite-element method for solving the problem of nonlinear coupling between interstitial pressure and temperature during stress on a poroelastic rock. Such coupling phenomena occur during massive injection of cold water into a petroleum borehole for example. The implementation of such a numerical solution, used here with the assumption of small deformations, first requires a review of the behavior law of the material (Eq. 2. 2 and of the equations for hydraulic diffusivity (Eq. 2. 3 and thermal diffusivity (Eq. 2. 4. This last equation (2. 4 is the one containing the nonlinear coupling terms in Grad P Grad P and Grad T. Grad P. During simulation of flow at a high flow rate, these products can no longer be neglected as shown by the results in Fig. 2. The variational formulation of the problem is then determined in relation to the three equations for equilibrium, thermal diffusivity and hydraulic diffusivity. After geometric and temporal discretizations, this formulation leads to a finite-element calculating scheme resulting in the simultaneous solving of all three equations. This solution, based on the inversion of the system of equations (2. 15, requires the updating of the rigidity matrix at each time step to take nonlinear coupling into consideration. Calculations with an

Radiosensitivity study in rice (Oriza Sativa Lin.)

International Nuclear Information System (INIS)

Gonzalez, M.; Santana, N.; Diaz, R.

1987-01-01

Four rice varieties (J-104, Amistad-82, 6066 and IR-1529) were irradiated at doses of 10,15,20,25,30,35,40,45,50 and 55 Krad of gamma rays so as to determine radiosensitivity curves for each of the varieties for the following factors; seed germination percentage; survival percentage; height of the plant; length of roots. It was determined that IR-1529 variety is the one with the highest sensitivity and that radiations over 35 Krad should not be used for none of the varieties above mentioned

Algorithms for Large-Scale Astronomical Problems

Science.gov (United States)

2013-08-01

Magana, Ross O’Connell, Xiaoying Xu, and Feng Yu . They are very nice and provided a lot of useful information. During these years I have worked with...Sicun Gao, Fan Guo, Yunchuan Kong, Ni Lao, Lei Li, Nan Li, Jialiu Lin, Yuefeng Lin, Xi Liu, Ning Lu, Min Luo, Kai Ren, Chao Shen, Long Qin, Huimin... Hsieh , Deborah A. Wallach, Mike Burrows, Tushar Chandra, Andrew Fikes, and Robert E. Gruber. Bigtable: A distributed storage system for structured data

Production of 48V in a nuclear reactor via secondary tritons

International Nuclear Information System (INIS)

Siri, S.; Cohen, I.M.

2009-01-01

The production of 48 V in a nuclear reactor, induced on titanium by tritons generated from the 6 Li(n, t) 4 He reaction, and eventually 7 Li(n, n't) 4 He, is described. Samples of lithium titanate were irradiated for an irradiation cycle (120 h) in the RA-3 reactor, belonging to Ezeiza Atomic Centre. After a radiochemical separation, the characteristic radiations from 48 V were identified in the gamma ray spectra of the vanadium fractions. (orig.)

Sensory integration dysfunction affects efficacy of speech therapy on children with functional articulation disorders

OpenAIRE

Tung, Li-Chen; Lin, Chin-Kai; Hsieh, Ching-Lin; Chen, Ching-Chi; Huang, Chin-Tsan; Wang, Chun-Hou

2013-01-01

Li-Chen Tung,1,# Chin-Kai Lin,2,# Ching-Lin Hsieh,3,4 Ching-Chi Chen,1 Chin-Tsan Huang,1 Chun-Hou Wang5,6 1Department of Physical Medicine and Rehabilitation, Chi Mei Medical Center, Tainan, 2Program of Early Intervention, Department of Early Childhood Education, National Taichung University of Education, Taichung, 3School of Occupational Therapy, College of Medicine, National Taiwan University, Taipei, 4Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, ...

Impulse Response of the Exchange Rate Volatility to a Foreign Exchange Intervention Shock

OpenAIRE

Hoshikawa, Takeshi

2009-01-01

This paper uses Lin's technique (1997) to report on the impulse response function analysis that traces the dynamics of exchange rate volatility from innovations in Japanese foreign exchange intervention. Using a multivariate GARCH model, we employed a volatility impulse response function based on Lin (1997) to detect the impulse response of exchange rate volatility on a one-unit foreign exchange intervention shock. The main findings of t his paper are as follows: (1) a foreign exchange inter...

Wobbled electronic properties of lithium clusters: Deterministic approach through first principles

Science.gov (United States)

Kushwaha, Anoop Kumar; Nayak, Saroj Kumar

2018-03-01

The innate tendency to form dendritic growth promoted through cluster formation leading to the failure of a Li-ion battery system have drawn significant attention of the researchers towards the effective destabilization of the cluster growth through selective implementation of electrolytic media such as acetonitrile (MeCN). In the present work, using first principles density functional theory and continuum dielectric model, we have investigated the origin of oscillatory nature of binding energy per atom of Lin (n ≤ 8) under the influence of MeCN. In the gas phase, we found that static mean polarizability is strongly correlated with binding energy and shows oscillatory nature with cluster size due to the open shell of Lin cluster. However, in acetonitrile medium, the binding energy has been correlated with electrostatic Lin -MeCN interaction and it has been found that both of them possess wobbled behavior characterized by the cluster size.

Polyunsaturated fatty acids combined with equine chorionic gonadotropin to enhance reproductive performance in aged rabbit does

Directory of Open Access Journals (Sweden)

Alaa E. Elkomy

2015-02-01

Full Text Available Sixty low-conception rate does aged 18 to 24 months (in four groups were used to determine the effect of replacement of prostaglandin (PG F2α (PGF2α injection by oral administration with sunflower oil (Sun (rich in omega 6 or linseed oil (Lin (rich in omega 3 on reproductive and productive performance. Group 1 was injected with 20 U of equine chorionic gonadotropin (eCG, 54 h before artificial insemination (AI and used as reference group. Group 2 was injected with 20 U of eCG+0.5 mg of PGF2α, 54 h before AI. Group 3 was orally given 3 mL of Sun/doe/day, for seven consecutive days before AI+20 U of eCG, 54 h before AI. Group 4 was treated like Group 3 except that the oil was Lin. Aged does treated with eCG+Sun had elevated blood 17-β estradiol concentration (P≤0.01 accompanied with a decrease of progesterone concentrations compared to the other experimental groups. Contrarily, no significant differences were found between eCG+Lin and eCG+PGF2α treatments on the previous two hormones. Likewise, aged does treated with eCG+Sun and eCG+Lin were statistically similar to those injected with ECG+synthetic PGF2α on blood prostaglandin profile, but still significantly higher than the control group. Treatment with eCG+Sun increased the percentage of fertile does (P≤0.01 and the litter size at birth compared to the other experimental groups. In conclusion, replacement of the PGF2α injection by oral administration of Sun or Lin to aged does improved sexual hormone synthesis and secretion, litter size and bunny body weight at birth.

Integrative taxonomy of Leptonetela spiders (Araneae, Leptonetidae, with descriptions of 46 new species

Directory of Open Access Journals (Sweden)

Chun-Xia Wang

2017-11-01

Full Text Available Extreme environments, such as subterranean habitats, are suspected to be responsible for morphologically inseparable cryptic or sibling species and can bias biodiversity assessment. A DNA barcode is a short, standardized DNA sequence used for taxonomic purposes and has the potential to lessen the challenges presented by a biotic inventory. Here, we investigate the diversity of the genus Leptonetela Kratochvíl, 1978 that is endemic to karst systems in Eurasia using DNA barcoding. We analyzed 624 specimens using one mitochondrial gene fragment (COI. The results show that DNA barcoding is an efficient and rapid species identification method in this genus. DNA barcoding gap and automatic barcode gap discovery (ABGD analyses indicated the existence of 90 species, a result consistent with previous taxonomic hypotheses, and supported the existence of extreme male pedipalpal tibial spine and median apophysis polymorphism in Leptonetela species, with direct implications for the taxonomy of the group and its diversity. Based on the molecular and morphological evidence, we delimit and diagnose 90 Leptonetela species, including the type species Leptonetela kanellisi (Deeleman-Reinhold, 1971. Forty-six of them are previously undescribed. The female of Leptonetela zhai Wang & Li, 2011 is reported for the first time. Leptonetela tianxinensis (Tong & Li, 2008 comb. nov. is transferred from the genus Leptoneta Simon, 1872; the genus Guineta Lin & Li, 2010 syn. nov. is a junior synonym of Leptonetela; Leptonetela gigachela (Lin & Li, 2010 comb. nov. is transferred from Guineta. The genus Sinoneta Lin & Li, 2010 syn. nov. is a junior synonym of Leptonetela; Leptonetela notabilis (Lin & Li, 2010 comb. nov. and Leptonetela sexdigiti (Lin & Li, 2010 comb. nov. are transferred from Sinoneta; Leptonetela sanchahe Wang & Li nom. nov. is proposed as a replacement name for Sinoneta palmata (Chen et al., 2010 because Leptonetela palmata is preoccupied.

Existence and uniqueness to the Cauchy problem for linear and semilinear parabolic equations with local conditions⋆

Directory of Open Access Journals (Sweden)

Rubio Gerardo

2011-03-01

Full Text Available We consider the Cauchy problem in ℝd for a class of semilinear parabolic partial differential equations that arises in some stochastic control problems. We assume that the coefficients are unbounded and locally Lipschitz, not necessarily differentiable, with continuous data and local uniform ellipticity. We construct a classical solution by approximation with linear parabolic equations. The linear equations involved can not be solved with the traditional results. Therefore, we construct a classical solution to the linear Cauchy problem under the same hypotheses on the coefficients for the semilinear equation. Our approach is using stochastic differential equations and parabolic differential equations in bounded domains. Finally, we apply the results to a stochastic optimal consumption problem. Nous considérons le problème de Cauchy dans ℝd pour une classe d’équations aux dérivées partielles paraboliques semi linéaires qui se pose dans certains problèmes de contrôle stochastique. Nous supposons que les coefficients ne sont pas bornés et sont localement Lipschitziennes, pas nécessairement différentiables, avec des données continues et ellipticité local uniforme. Nous construisons une solution classique par approximation avec les équations paraboliques linéaires. Les équations linéaires impliquées ne peuvent être résolues avec les résultats traditionnels. Par conséquent, nous construisons une solution classique au problème de Cauchy linéaire sous les mêmes hypothèses sur les coefficients pour l’équation semi-linéaire. Notre approche utilise les équations différentielles stochastiques et les équations différentielles paraboliques dans les domaines bornés. Enfin, nous appliquons les résultats à un problème stochastique de consommation optimale.

Integrative taxonomy of Leptonetela spiders (Araneae, Leptonetidae), with descriptions of 46 new species.

Science.gov (United States)

Wang, Chun-Xia; Xu, Xin; Li, Shu-Qiang

2017-11-18

Extreme environments, such as subterranean habitats, are suspected to be responsible for morphologically inseparable cryptic or sibling species and can bias biodiversity assessment. A DNA barcode is a short, standardized DNA sequence used for taxonomic purposes and has the potential to lessen the challenges presented by a biotic inventory. Here, we investigate the diversity of the genus Leptonetela Kratochvíl, 1978 that is endemic to karst systems in Eurasia using DNA barcoding. We analyzed 624 specimens using one mitochondrial gene fragment ( COI ). The results show that DNA barcoding is an efficient and rapid species identification method in this genus. DNA barcoding gap and automatic barcode gap discovery (ABGD) analyses indicated the existence of 90 species, a result consistent with previous taxonomic hypotheses, and supported the existence of extreme male pedipalpal tibial spine and median apophysis polymorphism in Leptonetela species, with direct implications for the taxonomy of the group and its diversity. Based on the molecular and morphological evidence, we delimit and diagnose 90 Leptonetela species, including the type species Leptonetela kanellisi (Deeleman-Reinhold, 1971). Forty-six of them are previously undescribed. The female of Leptonetela zhai Wang & Li, 2011 is reported for the first time. Leptonetela tianxinensis (Tong & Li, 2008) comb. nov. is transferred from the genus Leptoneta Simon, 1872; the genus Guineta Lin & Li, 2010 syn. nov. is a junior synonym of Leptonetela; Leptonetela gigachela (Lin & Li, 2010) comb. nov. is transferred from Guineta . The genus Sinoneta Lin & Li, 2010 syn. nov. is a junior synonym of Leptonetela ; Leptonetela notabilis (Lin & Li, 2010) comb. nov. and Leptonetela sexdigiti (Lin & Li, 2010) comb. nov. are transferred from Sinoneta ; Leptonetela sanchahe Wang & Li nom. nov. is proposed as a replacement name for Sinoneta palmata (Chen et al., 2010) because Leptonetela palmata is preoccupied.

47 CFR 27.1133 - Protection of Part 74 and Part 78 operations.

Science.gov (United States)

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Protection of Part 74 and Part 78 operations... MHz Bands Protection of Incumbent Operations § 27.1133 Protection of Part 74 and Part 78 operations... stations with those licensees. In the event that mutually satisfactory coordination agreements cannot be...

Renorming c0 and closed, bounded, convex sets with fixed point property for affine nonexpansive mappings

Science.gov (United States)

Nezir, Veysel; Mustafa, Nizami

2017-04-01

In 2008, P.K. Lin provided the first example of a nonreflexive space that can be renormed to have fixed point property for nonexpansive mappings. This space was the Banach space of absolutely summable sequences l1 and researchers aim to generalize this to c0, Banach space of null sequences. Before P.K. Lin's intriguing result, in 1979, Goebel and Kuczumow showed that there is a large class of non-weak* compact closed, bounded, convex subsets of l1 with fixed point property for nonexpansive mappings. Then, P.K. Lin inspired by Goebel and Kuczumow's ideas to give his result. Similarly to P.K. Lin's study, Hernández-Linares worked on L1 and in his Ph.D. thesis, supervisored under Maria Japón, showed that L1 can be renormed to have fixed point property for affine nonexpansive mappings. Then, related questions for c0 have been considered by researchers. Recently, Nezir constructed several equivalent norms on c0 and showed that there are non-weakly compact closed, bounded, convex subsets of c0 with fixed point property for affine nonexpansive mappings. In this study, we construct a family of equivalent norms containing those developed by Nezir as well and show that there exists a large class of non-weakly compact closed, bounded, convex subsets of c0 with fixed point property for affine nonexpansive mappings.

First-principles calculated decomposition pathways for LiBH4 nanoclusters

Science.gov (United States)

Huang, Zhi-Quan; Chen, Wei-Chih; Chuang, Feng-Chuan; Majzoub, Eric H.; Ozoliņš, Vidvuds

2016-05-01

We analyze thermodynamic stability and decomposition pathways of LiBH4 nanoclusters using grand-canonical free-energy minimization based on total energies and vibrational frequencies obtained from density-functional theory (DFT) calculations. We consider (LiBH4)n nanoclusters with n = 2 to 12 as reactants, while the possible products include (Li)n, (B)n, (LiB)n, (LiH)n, and Li2BnHn; off-stoichiometric LinBnHm (m ≤ 4n) clusters were considered for n = 2, 3, and 6. Cluster ground-state configurations have been predicted using prototype electrostatic ground-state (PEGS) and genetic algorithm (GA) based structural optimizations. Free-energy calculations show hydrogen release pathways markedly differ from those in bulk LiBH4. While experiments have found that the bulk material decomposes into LiH and B, with Li2B12H12 as a kinetically inhibited intermediate phase, (LiBH4)n nanoclusters with n ≤ 12 are predicted to decompose into mixed LinBn clusters via a series of intermediate clusters of LinBnHm (m ≤ 4n). The calculated pressure-composition isotherms and temperature-pressure isobars exhibit sloping plateaus due to finite size effects on reaction thermodynamics. Generally, decomposition temperatures of free-standing clusters are found to increase with decreasing cluster size due to thermodynamic destabilization of reaction products.

Comparison between Two Linear Supervised Learning Machines' Methods with Principle Component Based Methods for the Spectrofluorimetric Determination of Agomelatine and Its Degradants.

Science.gov (United States)

Elkhoudary, Mahmoud M; Naguib, Ibrahim A; Abdel Salam, Randa A; Hadad, Ghada M

2017-05-01

Four accurate, sensitive and reliable stability indicating chemometric methods were developed for the quantitative determination of Agomelatine (AGM) whether in pure form or in pharmaceutical formulations. Two supervised learning machines' methods; linear artificial neural networks (PC-linANN) preceded by principle component analysis and linear support vector regression (linSVR), were compared with two principle component based methods; principle component regression (PCR) as well as partial least squares (PLS) for the spectrofluorimetric determination of AGM and its degradants. The results showed the benefits behind using linear learning machines' methods and the inherent merits of their algorithms in handling overlapped noisy spectral data especially during the challenging determination of AGM alkaline and acidic degradants (DG1 and DG2). Relative mean squared error of prediction (RMSEP) for the proposed models in the determination of AGM were 1.68, 1.72, 0.68 and 0.22 for PCR, PLS, SVR and PC-linANN; respectively. The results showed the superiority of supervised learning machines' methods over principle component based methods. Besides, the results suggested that linANN is the method of choice for determination of components in low amounts with similar overlapped spectra and narrow linearity range. Comparison between the proposed chemometric models and a reported HPLC method revealed the comparable performance and quantification power of the proposed models.

Field lin topology in the dayside cusp region inferred from low altitude particle observations

International Nuclear Information System (INIS)

Soeraas, F.

1977-12-01

Dayside low altitude satellite observations of the pitch angle and energy distribution of electrons and protons in the energy range 1 keV to 100 keV during quiet geomagnetic conditions reveal that at times there is a clear latitudinal separation between the precipitating low energy (keV) electrons and protons, with the protons precipitating poleward of the electrons. The high energy (100keV) proton precipitation overlaps both the low energy electron and proton precipitation. These observations are consistent with a model where magnetosheath particles stream in along the cusp field lines and are at the same time convected poleward by an electric field. Electrons with energies of a few keV move fast and give the ''ionospheric footprint'' of the distant cusp. The protons are partly convected poleward of the cusp and into the polar cap. Here the mirroring protons populate the plasma mantle. Equatorward of the cusp the pitch angle distribution of both electrons and protons with energies above a few keV have a pancake shaped distribution indicating closed geomagnetic field lines. The 1 keV electrons penetrate into this region of closed field line structure maintaining an isotropic pitch angle distribution. The intensity is, however, reduced with respect to what it was in the cusp region. It is suggested that these electrons, the lowest measured on the satellite, are associated with the entry layer.(Auth.)

Motorcycle Parts

Science.gov (United States)

1993-01-01

An article in NASA Tech Briefs describing a vacuum bagging process for forming composite parts helped a small Oklahoma Company to improve its manufacturing process. President of Performance Extremes, Larry Ortega, and his partners make motorcycle parts from carbon/epoxy to reduce weight. Using vacuum bags, parts have a better surface and fewer voids inside. When heat used in the vacuum bag process caused deformation upon cooling, a solution found in another tech brief solved the problem. A metal plate inside the vacuum bag made for more even heat transfer. A third article described a simple procedure for repairing loose connector pins, which the company has also utilized.

MAI-free performance of PMU-OFDM transceiver in time-variant environment

Science.gov (United States)

Tadjpour, Layla; Tsai, Shang-Ho; Kuo, C.-C. J.

2005-06-01

An approximately multi-user OFDM transceiver was introduced to reduce the multi-access interference (MAI ) due to the carrier frequency offset (CFO) to a negligible amount via precoding by Tsai, Lin and Kuo. In this work, we investigate the performance of this precoded multi-user (PMU) OFDM system in a time-variant channel environment. We analyze and compare the MAI effect caused by time-variant channels in the PMU-OFDM and the OFDMA systems. Generally speaking, the MAI effect consists of two parts. The first part is due to the loss of orthogonality among subchannels for all users while the second part is due to the CFO effect caused by the Doppler shift. Simulation results show that, although OFDMA outperforms the PMU-OFDM transceiver in a fast time-variant environment without CFO, PMU-OFDM outperforms OFDMA in a slow time-variant channel via the use of M/2 symmetric or anti-symmetric codewords of M Hadamard-Walsh codes.

Borussica

Directory of Open Access Journals (Sweden)

Letas Palmaitis

2011-10-01

Full Text Available Dėl formos kaūlins kilmės prūsų katekizmų kalbojeSantraukaPrūsų kalboje galūnės –C’an/-s ir –Cin/-s buvo akuzatyvo alomorfai, o kadangi l koreliacijos pagal minkštumą nebuvo ir l buvo suvokiamas kaip „minkštas", visi a-kamieniai žodžiai su tuo paskutiniuoju priebalsiu buvo suvokiami tarsi ja-/i-kamieniai: acc. pl. kaūlins.

[Being personal: the development of community psychiatric mental health nursing].

Science.gov (United States)

Shiau, Shu-Jen; Lee, Shu-Hong

2009-08-01

Community psychiatric mental health nursing care emphasizes humanistic values and focuses on serving patient and family needs. In Taiwan, such care is delivered largely as part of patient discharge care plans and hospital / community based service models. Issues involved underscore the importance of operating an effective and integrated transfer system, the role and function of nurses and training in relevant competencies (Shiau, Huang & Lin, 2005). This article again emphasizes the importance of 'being personal' in the development of community psychiatric mental health nursing in Taiwan. Critical issues to consider include humanization, empowerment, nursing competencies, regulations, relating on a personal level, and facilitating empowerment and enlightenment on the healing process.

Characterization studies of lithium vapour generated in heat pipe oven for the Plasma Wakefield Accelerator Experiment

International Nuclear Information System (INIS)

Mohandas, K.K.; Mahavar, Kanchan; Ajai Kumar; Kumar, Ravi A.V.

2013-01-01

Characterization and optimization studies of lithium vapor by white light as well as UV laser absorption were carried out as part of generation of photo ionized Li plasma for the Plasma Wake Field Acceleration Experiment. Temperature and buffer gas pressure dependency of the neutral density of lithium vapor was studied in detail. The line integrated neutral density of Li(n o L) was found to be of the order of 10 17 -10 18 cm -2 at heat pipe oven temperatures in the range from 600-800℃ which is sufficient to obtain the required 1013-1014 cm -3 plasma densities by photo ionization. (author)

Soldadura por fricción lineal de las aleaciones de titanio: caracterización de una soldadura longitudinal mediante ensayos a tracción

OpenAIRE

Grand, Arnaud

2006-01-01

Doble titulació La unión entre los álabes y el disco de una turbina de avión está en plena evolución por la aplicación del proceso de soldadura por fricción lineal (LFW) a las aleaciones de titanio. Ello permite conseguir una pieza única llamada blisk (blade + disk). Hasta ahora, la unión álabe / disco se hacía por un ensamblaje mecánico, pero estos sufren un fenómeno de fatiga por fretting en el punto de anclaje. A parte de evitar ese fenómeno, la soldadura por fricción lin...

REPRESENTATIONS NON LINEAIRES DE L'AUTOMATIQUE AVANCEE. APPLICATION AU MOTEUR DIESEL TURBOCOMPRESSE

Directory of Open Access Journals (Sweden)

A LIAZID

2000-12-01

Full Text Available Cet article décrit et commente les principaux modèles non linéaires de représentation, définis en temps discret. Il s'agit des séries fonctionnelles de Volterra, des modèles décrits dans l'espace vectoriel d'état, des systèmes à non linéarités séparées et des modèles sous forme d'équations aux différences. Ceci offrira aux utilisateurs un aperçu pratique sur les choix à réaliser par rapport aux objectifs liés à l'exploitation de ces modèles: simulation ou commande. Quelques exemples portant sur l'identification non linéaire du comportement dynamique du moteur Diesel suralimenté par turbocompresseur, illustrent l'intérêt des modèles NARMAX (Non linear Auto Regressive Moving Average with eXogenous inputs, notamment par leur puissance prédictive.

Standard biological parts knowledgebase.

Directory of Open Access Journals (Sweden)

Michal Galdzicki

2011-02-01

Full Text Available We have created the Knowledgebase of Standard Biological Parts (SBPkb as a publically accessible Semantic Web resource for synthetic biology (sbolstandard.org. The SBPkb allows researchers to query and retrieve standard biological parts for research and use in synthetic biology. Its initial version includes all of the information about parts stored in the Registry of Standard Biological Parts (partsregistry.org. SBPkb transforms this information so that it is computable, using our semantic framework for synthetic biology parts. This framework, known as SBOL-semantic, was built as part of the Synthetic Biology Open Language (SBOL, a project of the Synthetic Biology Data Exchange Group. SBOL-semantic represents commonly used synthetic biology entities, and its purpose is to improve the distribution and exchange of descriptions of biological parts. In this paper, we describe the data, our methods for transformation to SBPkb, and finally, we demonstrate the value of our knowledgebase with a set of sample queries. We use RDF technology and SPARQL queries to retrieve candidate "promoter" parts that are known to be both negatively and positively regulated. This method provides new web based data access to perform searches for parts that are not currently possible.

Standard Biological Parts Knowledgebase

Science.gov (United States)

Galdzicki, Michal; Rodriguez, Cesar; Chandran, Deepak; Sauro, Herbert M.; Gennari, John H.

2011-01-01

We have created the Knowledgebase of Standard Biological Parts (SBPkb) as a publically accessible Semantic Web resource for synthetic biology (sbolstandard.org). The SBPkb allows researchers to query and retrieve standard biological parts for research and use in synthetic biology. Its initial version includes all of the information about parts stored in the Registry of Standard Biological Parts (partsregistry.org). SBPkb transforms this information so that it is computable, using our semantic framework for synthetic biology parts. This framework, known as SBOL-semantic, was built as part of the Synthetic Biology Open Language (SBOL), a project of the Synthetic Biology Data Exchange Group. SBOL-semantic represents commonly used synthetic biology entities, and its purpose is to improve the distribution and exchange of descriptions of biological parts. In this paper, we describe the data, our methods for transformation to SBPkb, and finally, we demonstrate the value of our knowledgebase with a set of sample queries. We use RDF technology and SPARQL queries to retrieve candidate “promoter” parts that are known to be both negatively and positively regulated. This method provides new web based data access to perform searches for parts that are not currently possible. PMID:21390321

Standard biological parts knowledgebase.

Science.gov (United States)

Galdzicki, Michal; Rodriguez, Cesar; Chandran, Deepak; Sauro, Herbert M; Gennari, John H

2011-02-24

We have created the Knowledgebase of Standard Biological Parts (SBPkb) as a publically accessible Semantic Web resource for synthetic biology (sbolstandard.org). The SBPkb allows researchers to query and retrieve standard biological parts for research and use in synthetic biology. Its initial version includes all of the information about parts stored in the Registry of Standard Biological Parts (partsregistry.org). SBPkb transforms this information so that it is computable, using our semantic framework for synthetic biology parts. This framework, known as SBOL-semantic, was built as part of the Synthetic Biology Open Language (SBOL), a project of the Synthetic Biology Data Exchange Group. SBOL-semantic represents commonly used synthetic biology entities, and its purpose is to improve the distribution and exchange of descriptions of biological parts. In this paper, we describe the data, our methods for transformation to SBPkb, and finally, we demonstrate the value of our knowledgebase with a set of sample queries. We use RDF technology and SPARQL queries to retrieve candidate "promoter" parts that are known to be both negatively and positively regulated. This method provides new web based data access to perform searches for parts that are not currently possible.

Loose part monitoring device

International Nuclear Information System (INIS)

Nomura, Hiroshi.

1992-01-01

The device of the present invention estimates a place where loose parts occur and structural components as the loose parts in a fluid flow channel of a reactor device, to provide information thereof to a plant operator. That is, the device of the present invention comprises (1) a plurality of detectors disposed to each of equipments constituting fluid channels, (2) an abnormal sound sensing device for sensing signals from the detectors, (3) an estimation section for estimating the place where the loose parts occur and the structural components thereof based on the signals sensed by the abnormal sound sensing section, (4) a memory section for storing data of the plant structure necessary for the estimation, and (5) a display section for displaying the result of the estimation. In such a device, the position where the loose parts collide against the plant structural component and the energy thereof are estimated. The dropping path of the loose parts is estimated from the estimation position. Parts to be loose parts in the path are listed up. The parts on the list is selected based on the estimated energy thereby enabling to determine the loose parts. (I.S.)

Bone Shaft Revascularization After Marrow Ablation Is Dramatically Accelerated in BSP-/- Mice, Along With Faster Hematopoietic Recolonization.

Science.gov (United States)

Bouleftour, Wafa; Granito, Renata Neves; Vanden-Bossche, Arnaud; Sabido, Odile; Roche, Bernard; Thomas, Mireille; Linossier, Marie Thérèse; Aubin, Jane E; Lafage-Proust, Marie-Hélène; Vico, Laurence; Malaval, Luc

2017-09-01

The bone organ integrates the activity of bone tissue, bone marrow, and blood vessels and the factors ensuring this coordination remain ill defined. Bone sialoprotein (BSP) is with osteopontin (OPN) a member of the small integrin binding ligand N-linked glycoprotein (SIBLING) family, involved in bone formation, hematopoiesis and angiogenesis. In rodents, bone marrow ablation induces a rapid formation of medullary bone which peaks by ∼8 days (d8) and is blunted in BSP-/- mice. We investigated the coordinate hematopoietic and vascular recolonization of the bone shaft after marrow ablation of 2 month old BSP+/+ and BSP-/- mice. At d3, the ablated area in BSP-/- femurs showed higher vessel density (×4) and vascular volume (×7) than BSP+/+. Vessel numbers in the shaft of ablated BSP+/+ mice reached BSP-/- values only by d8, but with a vascular volume which was twice the value in BSP-/-, reflecting smaller vessel size in ablated mutants. At d6, a much higher number of Lin - (×3) as well as LSK (Lin - IL-7Rα - Sca-1 hi c-Kit hi , ×2) and hematopoietic stem cells (HSC: Flt3 - LSK, ×2) were counted in BSP-/- marrow, indicating a faster recolonization. However, the proportion of LSK and HSC within the Lin - was lower in BSP-/- and more differentiated stages were more abundant, as also observed in unablated bone, suggesting that hematopoietic differentiation is favored in the absence of BSP. Interestingly, unablated BSP-/- femur marrow also contains more blood vessels than BSP+/+, and in both intact and ablated shafts expression of VEGF and OPN are higher, and DMP1 lower in the mutants. In conclusion, bone marrow ablation in BSP-/- mice is followed by a faster vascular and hematopoietic recolonization, along with lower medullary bone formation. Thus, lack of BSP affects the interplay between hematopoiesis, angiogenesis, and osteogenesis, maybe in part through higher expression of VEGF and the angiogenic SIBLING, OPN. J. Cell. Physiol. 232: 2528-2537, 2017. © 2016

DISCRETIZATION APPROACH USING RAY-TESTING MODEL IN PARTING LINE AND PARTING SURFACE GENERATION

Institute of Scientific and Technical Information of China (English)

HAN Jianwen; JIAN Bin; YAN Guangrong; LEI Yi

2007-01-01

Surface classification, 3D parting line, parting surface generation and demoldability analysis which is helpful to select optimal parting direction and optimal parting line are involved in automatic cavity design based on the ray-testing model. A new ray-testing approach is presented to classify the part surfaces to core/cavity surfaces and undercut surfaces by automatic identifying the visibility of surfaces. A simple, direct and efficient algorithm to identify surface visibility is developed. The algorithm is robust and adapted to rather complicated geometry, so it is valuable in computer-aided mold design systems. To validate the efficiency of the approach, an experimental program is implemented. Case studies show that the approach is practical and valuable in automatic parting line and parting surface generation.

Production of {sup 48}V in a nuclear reactor via secondary tritons

Energy Technology Data Exchange (ETDEWEB)

Siri, S. [Comision Nacional de Energia Atomica, Centro Atomico Ezeiza, Gerencia de Capacitacion, Quimica Nuclear y Ciencias de la Salud, Ezeiza, Buenos Aires (Argentina); Cohen, I.M. [Univ. Tecnologica Nacional, Dept. de Ingenieria Quimica, Buenos Aires (Argentina)

2009-07-01

The production of {sup 48}V in a nuclear reactor, induced on titanium by tritons generated from the {sup 6}Li(n, t){sup 4} He reaction, and eventually {sup 7}Li(n, n't){sup 4}He, is described. Samples of lithium titanate were irradiated for an irradiation cycle (120 h) in the RA-3 reactor, belonging to Ezeiza Atomic Centre. After a radiochemical separation, the characteristic radiations from {sup 48}V were identified in the gamma ray spectra of the vanadium fractions. (orig.)

Commonsense in parts : Mining part-whole relations from theweb and image tags

NARCIS (Netherlands)

Tandon, Niket; Hariman, Charles; Urbani, Jacopo; Rohrbach, Anna; Rohrbach, Marcus; Weikum, Gerhard

2016-01-01

Commonsense knowledge about part-whole relations (e.g., screen partOf notebook) is important for interpreting user input in web search and question answering, or for object detection in images. Prior work on knowledge base construction has compiled part-whole assertions, but with substantial

Improving automatic earthquake locations in subduction zones: a case study for GEOFON catalog of Tonga-Fiji region

Science.gov (United States)

Nooshiri, Nima; Heimann, Sebastian; Saul, Joachim; Tilmann, Frederik; Dahm, Torsten

2015-04-01

Automatic earthquake locations are sometimes associated with very large residuals up to 10 s even for clear arrivals, especially for regional stations in subduction zones because of their strongly heterogeneous velocity structure associated. Although these residuals are most likely not related to measurement errors but unmodelled velocity heterogeneity, these stations are usually removed from or down-weighted in the location procedure. While this is possible for large events, it may not be useful if the earthquake is weak. In this case, implementation of travel-time station corrections may significantly improve the automatic locations. Here, the shrinking box source-specific station term method (SSST) [Lin and Shearer, 2005] has been applied to improve relative location accuracy of 1678 events that occurred in the Tonga subduction zone between 2010 and mid-2014. Picks were obtained from the GEOFON earthquake bulletin for all available station networks. We calculated a set of timing corrections for each station which vary as a function of source position. A separate time correction was computed for each source-receiver path at the given station by smoothing the residual field over nearby events. We begin with a very large smoothing radius essentially encompassing the whole event set and iterate by progressively shrinking the smoothing radius. In this way, we attempted to correct for the systematic errors, that are introduced into the locations by the inaccuracies in the assumed velocity structure, without solving for a new velocity model itself. One of the advantages of the SSST technique is that the event location part of the calculation is separate from the station term calculation and can be performed using any single event location method. In this study, we applied a non-linear, probabilistic, global-search earthquake location method using the software package NonLinLoc [Lomax et al., 2000]. The non-linear location algorithm implemented in NonLinLoc is less

Prime Contract Awards by State or Country, Place and Contractor. Part 3 (Lodi, California - Saint Helena, California), FY1991

Science.gov (United States)

1991-01-01

8217 00𔃻) 0- .-4-4-4-𔃾 0D01l CN a xZI-Zx a 1.- 00 P- CD gao -s4Nnm-4 LIn n cn C’.j C1 00 NI (0 a 9xU0I- a C. 00 0 -0 0C 000C>-4 00 4 0 0 0D 0-4 * L LAJ4...0) .1 2(0(0-i Of Nf I(Do0N NO 4) < 0 -4 -C 04 - 4 -N 1 0N a 1- 16- 24 C.) 0 - IL I JINI I(0 Naci S 0 0 I-40) 2 0 I (0 C N I0N NC 1- m- C 1-1 1.- 0 I...7.1൰ .C. CIANNN F-- A-4 -4-4 -4 L--4 -4 M -A W-4 it w 0 (0.-I #I <(0 0(0 (0) "-00 00000 000000 coc o 0 ɘ) 0) ) 0)m0) 4D a) M 0( 11 U. I M0-4 aI - jIni

Bone marrow-derived mesenchymal stem cells propagate immunosuppressive/anti-inflammatory macrophages in cell-to-cell contact-independent and -dependent manners under hypoxic culture.

Science.gov (United States)

Takizawa, Naoki; Okubo, Naoto; Kamo, Masaharu; Chosa, Naoyuki; Mikami, Toshinari; Suzuki, Keita; Yokota, Seiji; Ibi, Miho; Ohtsuka, Masato; Taira, Masayuki; Yaegashi, Takashi; Ishisaki, Akira; Kyakumoto, Seiko

2017-09-15

Immunosuppressive/anti-inflammatory macrophage (Mφ), M2-Mφ that expressed the typical M2-Mφs marker, CD206, and anti-inflammatory cytokine, interleukin (IL)-10, is beneficial and expected tool for the cytotherapy against inflammatory diseases. Here, we demonstrated that bone marrow-derived lineage-positive (Lin+) blood cells proliferated and differentiated into M2-Mφs by cooperation with the bone marrow-derived mesenchymal stem cells (MSCs) under hypoxic condition: MSCs not only promoted proliferation of undifferentiated M2-Mφs, pre-M2-Mφs, in the Lin+ fraction via a proliferative effect of the MSCs-secreted macrophage colony-stimulating factor, but also promoted M2-Mφ polarization of the pre-M2-Mφs through cell-to-cell contact with the pre-M2-Mφs. Intriguingly, an inhibitor for intercellular adhesion molecule (ICAM)-1 receptor/lymphocyte function-associated antigen (LFA)-1, Rwj50271, partially suppressed expression of CD206 in the Lin+ blood cells but an inhibitor for VCAM-1 receptor/VLA-4, BIO5192, did not, suggesting that the cell-to-cell adhesion through LFA-1 on pre-M2-Mφs and ICAM-1 on MSCs was supposed to promoted the M2-Mφ polarization. Thus, the co-culture system consisting of bone marrow-derived Lin+ blood cells and MSCs under hypoxic condition was a beneficial supplier of a number of M2-Mφs, which could be clinically applicable to inflammatory diseases. Copyright © 2017 Elsevier Inc. All rights reserved.

Modulating Leukemia-Initiating Cell Quiescence to Improve Leukemia Treatment

Science.gov (United States)

2015-09-01

T- cells and in innate immunity (Lacorazza et al., 2002). It controls the proliferation and homing of CD8+ T- cells via the Kruppel-like factors...Lin2Sca12IL7R2Kit1FccRII/ IIIhighCD34high), megakaryocyte-erythroid progenitor cell (MEP) (Lin2Sca12IL7R2Kit1FccRII/IIIlowCD34low), and common lymphoid ...to this model, the first wave gives rise exclusively to innate immune B cells in early embryonic life and may be derived from progenitor cells

The CRREL Instrumented Vehicle: Hardware and Software.

Science.gov (United States)

1983-01-01

PRINT CHRS( 1 ’ 57Ŕ PRINT LINkI ) � I NPuT "DO ,OU ST ILL WANT A TPA.T ION VALUE F ’f N’’’",Pp I 5750 IF RpISl"N" THEN GOTO 6510 57,?0 PRINT PAGE... LINkI ,"TYPEOR UNDEPL,ING SURFACE: "Te9’ 7’ PRINT LIN( 1,"DEPTN OR THICaNESS: " T, Sc,;" CM" -o PRINT LIN, I’,"MATEPIAL TEMP: "TS’ II 1*’ DEC C",L:’’ I

Stemcell Information: SKIP000144 [SKIP Stemcell Database[Archive

Lifescience Database Archive (English)

Full Text Available ... Makoto Otsu --Huan-Ting Lin 大津　真--Huan-Ting Lin University of Tokyo, Institute of Medical Science--U...niversity of Tokyo, Institute of Medical Science 東京大学医科学研究所--東京大学医科学研究所 Universit...y of Tokyo, Institute of Medical Science 東京大学医科学研究所 Makoto Otsu 大津　真 Not Available Riken BRC 理研BRC http://www.brc.riken.jp/lab/cell/english/patient_specific_ips.shtml ...

Stemcell Information: SKIP000145 [SKIP Stemcell Database[Archive

Lifescience Database Archive (English)

Full Text Available ... Makoto Otsu --Huan-Ting Lin 大津　真--Huan-Ting Lin University of Tokyo, Institute of Medical Science--Un...iversity of Tokyo, Institute of Medical Science 東京大学医科学研究所--東京大学医科学研究所 University... of Tokyo, Institute of Medical Science 東京大学医科学研究所 Makoto Otsu 大津　真 Not Available Riken BRC 理研BRC http://www.brc.riken.jp/lab/cell/english/patient_specific_ips.shtml ...

Impact du comportement vibratoire et acoustique des joints de portes et de vitrages sur le bruit d'origine aérodynamique

OpenAIRE

Oliver , Clara; Jézéquel , Louis; Besset , Sébastien; VAN HERPE , François; Abbadi , Zouhir

2015-01-01

International audience; Dans la modélisation des joints de portes et de vitrages pour la prédiction du bruit aérodynamique il est nécessaire de sortir du domaine classique du calcul vibro-acoustique linéaire, en raison du caractère fortement non-linéaire des joints. La déformation lors de la fermeture des portes ou vitres provoque des modifications des raideurs statique et dynamique, de l'amortissement et de la transparence acoustique des joints. Ensuite les efforts aérodynamiques modifient c...

Air-oxidized linalool-a frequent cause of fragrance contact allergy

DEFF Research Database (Denmark)

Bråred Christensson, Johanna; Andersen, Klaus Ejner; Bruze, Magnus

2012-01-01

patch test reactions in 2500 dermatitis patients. Objectives. To investigate whether oxidized linalool 6%, with a stable concentration of the main haptens, the linalool hydroperoxides (Lin-OOHs) in pet., could be a useful tool for the detection of contact allergy in an international setting. Methods....... Oxidized linalool 6.0% (Lin-OOHs 1%) pet. was tested in 2900 consecutive dermatitis patients in Denmark, the United Kingdom, Singapore, Spain, Sweden, and Australia. Results. Overall, 6.9% (range 3-13%) of the patients showed positive patch test reactions to oxidized linalool. Doubtful reactions were found...

Otvetshajet Juhan Parts, premjer-ministr / Juhan Parts

Index Scriptorium Estoniae

Parts, Juhan, 1966-

2005-01-01

Peaminister Juhan Parts selgitab Res Publica ja valitsuse korruptsioonivastast poliitikat ning loodab, et justiitsminister Ken-Marti Vaheri pakutud strateegia võitluses korruptsiooniga ei saa koalitsiooni lagunemise põhjuseks

Part-Time Faculty Satisfaction at Two-Year Public Postsecondary Institutions: A Comparison of Involuntary Part-Time, Voluntary Part-Time, and Full-Time Faculty

Science.gov (United States)

Kinchen, Nancy Huval

2010-01-01

Part-time faculty members represent the majority of faculty at public two-year postsecondary institutions. Utilizing part-time faculty enables two-year institutions to control their instructional costs and maintain scheduling flexibility. However, part-time faculty are diverse in regards to their employment preference, some prefer part-time…

The effectiveness of parent participation in occupational therapy for children with developmental delay

Directory of Open Access Journals (Sweden)

Lin CL

2018-02-01

Full Text Available Chien-Lin Lin1,2 Chin-Kai Lin,3 Jia-Jhen Yu4 1Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 2School of Chinese Medicine, College of Chinese Medicine, China Medical University, 3Program of Early Intervention, Department of Early Childhood Education, National Taichung University of Education, 4Occupation Therapy Unit of Rehabilitation Techniques Section, Lin Shin Medical Corporation Wuri Lin Shin Hospital, Taichung, Taiwan Introduction: This study aims to explore the impact of Parent Participation Program on the development of developmental delay children. Methods: Pretest-posttest equivalent-group experimental design study was used in this paper. A total of 30 pairs of developmental delay children aged 0–72 months and their parents participated into this study. They were divided into two groups, namely control group and experimental group, according to parents’ wishes. The objects of study in control group received 16 courses of direct rehabilitation therapy; those in experimental group received 8 courses of direct rehabilitation therapy and 8 courses of instruction and tracking of Parent Participation Program. The duration of the intervention was 8 weeks. All cases should be evaluated before and after the intervention, to analyze the difference before and after intervention and among groups. The statistical methods in this paper included descriptive analysis, Chi-square test, independent sample t-test, pair-sample t-test. Results and conclusion: The intervention of Parent Participation Occupational Program has positive impact on the development of developmental delay children in various fields. Among all the intervention results, the progress of the experimental group is 1.895 times more than that of the control group. With parent involvement, Parent Participation Occupational Therapy can promote the cognitive ability, language ability, action ability (gross and fine movement, social competence and

Fabrification of electroreduced graphene oxide–bentonite sodium composite modified electrode and its sensing application for linezolid

International Nuclear Information System (INIS)

Prashanth, S.N.; Teradal, Nagappa L.; Seetharamappa, J.; Satpati, Ashis K.; Reddy, A.V.R.

2014-01-01

Graphene and its composites have attracted considerable attention in synthesis and electrochemical applications. In the present work, we have synthesized and characterized graphene oxide-bentonite composite (GO-BEN) and utilized it to fabricate an electrochemical sensor. For this, the solution of GO-BEN cast on glassy carbon electrode (GCE) was reduced electrochemically in phosphate buffer solution of pH 6 to obtain electrochemically reduced graphene oxide-bentonite composite (ERGO-BEN-GCE). This ERGO-BEN film was used for electrochemical investigation of an oxazolidinone class of antibiotic, linezolid (LIN) for the first time. The electrochemical sensor showed excellent enhancement and adsorptive ability towards the electrooxidation of LIN. LIN exhibited two each of oxidation and reduction peaks on ERGO-BEN film in phosphate buffer of pH 7.0. Effects of accumulation time, pH of solution and scan rate were studied and various electrochemical parameters were evaluated. The plot of pH versus E p gave a slope of 26.2 mV/pH in the pH range of 4.2-8.0 indicating the participation of two electrons and one proton in the electrode process. An adsorptive stripping differential pulse voltammetric method (AdSDPV) was developed for the determination of LIN in bulk, pharmaceutical formulations and urine samples. Adsorptive stripping linear sweep voltammetric (AdSLSV) and differential pulse voltammetric (DPV) methods were also developed and the results were compared. LIN showed linear relationship between the current density and concentration in the range of 0.25 - 31.25 μM with a LOD of 0.0337 μM in AdSDPV method; 0.5 - 31.25 μM with a LOD of 0.100 μM in DPV method and 1.25 - 37.5 μM with a LOD of 0.5461 μM in AdSLSV method respectively. The proposed AdSDPV method was observed to be simple, fast and inexpensive and hence, could be readily adopted for quality control in pharmaceutical products

NASA EEE Parts and NASA Electronic Parts and Packaging (NEPP) Program Update 2018

Science.gov (United States)

Label, Kenneth A.; Sampson, Michael J.; Pellish, Jonathan A.; Majewicz, Peter J.

2018-01-01

NASA Electronic Parts and Packaging (NEPP) Program and NASA Electronic Parts Assurance Group (NEPAG) are NASAs point-of-contacts for reliability and radiation tolerance of EEE parts and their packages. This presentation includes an FY18 program overview.

Play your part

CERN Document Server

Ramsey, Gaynor

1978-01-01

Play your part is a collection of then situations in which students have to take on the roles of particular people and express their opinions, feelings or arguments about the situation. Play your part is intended for use with advanced students of English.

Pushing for Part Time.

Science.gov (United States)

Geber, Beverly

1987-01-01

More employees are choosing to work part time. Although this trend started because of working women, some men are choosing part-time positions. Part-time employees forfeit salary and promotion potential, yet most feel the trade is fair and that they are more productive during their working hours. (CH)

Comportement mécanique des tuyaux. Influence de la traction, de la pression et du poids linéique. Application aux risers Mechanical Behavior of Pipes and Risers. Influence of Tension, Pressure and Weight

Directory of Open Access Journals (Sweden)

Sparks C.

2006-11-01

Full Text Available L'influence de la traction, de la pression et du poids linéique sur le comportement d'un tuyau (ou d'un riser a déjà été traitée dans de nombreux articles. Malgré cela, elle est fréquemment mal comprise, ce qui a parfois de; conséquences sérieuses. Par souci de clarté, l'approche de cet article est délibérément très élémentaire. Les problèmes principaux de la flexion, du flambage, de l'allongement et de la contrainte limite, qui sont souvent traités dans des articles différents, sont ici étudiés ensemble d'une façon homogène. On présente des interprétation: physiques, faciles à visualiser, des deux concepts de tension effective et de contrainte effective, dont l'utilisation est très répandue, ainsi que leur rapport avec des notions analogues dans d'autres domaines de l'art de l'ingénieur. La puissance de ces deux concepts est démontrée à la fin de l'article où ils sont utilisés pour résoudre huit problèmes divers. The influence of tension, pressure, and weight on different aspects of pipe and riser behavior has already been the subject of many articles (see references. In spite of this it has frequently been misunderstood, sometimes with serious consequences. The object of this payer is to explain the subject clearly and the approach is therefore deliberately very elementary. The principal problems of bending, buckling, yielding and strains, usually treated in separated papers, are here treated homogeneously. The widely used concepts of effective tension and effective stress are given interpretations, easy to visualise, which are related to analogous concepts in other engineering fields. Finally, eight diverse particular examples of the influence of tension, pressure and weight on pipe and riser behavior, are discussed.

Part C and Part D Compliance Actions

Data.gov (United States)

U.S. Department of Health & Human Services — This page includes results from CMSs Past Performance Review. The report called Spring 2014 Past Performance Review Outlier Results lists MA organizations and Part D...

Parts Quality Management: Direct Part Marking of Data Matrix Symbol for Mission Assurance

Science.gov (United States)

Moss, Chantrice; Chakrabarti, Suman; Scott, David W.

2013-01-01

A United States Government Accountability Office (GAO) review of twelve NASA programs found widespread parts quality problems contributing to significant cost overruns, schedule delays, and reduced system reliability. Direct part marking with Data Matrix symbols could significantly improve the quality of inventory control and parts lifecycle management. This paper examines the feasibility of using direct part marking technologies for use in future NASA programs. A structural analysis is based on marked material type, operational environment (e.g., ground, suborbital, Low Earth Orbit), durability of marks, ease of operation, reliability, and affordability. A cost-benefits analysis considers marking technology (label printing, data plates, and direct part marking) and marking types (two-dimensional machine-readable, human-readable). Previous NASA parts marking efforts and historical cost data are accounted for, including inhouse vs. outsourced marking. Some marking methods are still under development. While this paper focuses on NASA programs, results may be applicable to a variety of industrial environments.

First Jurassic grasshopper (Insecta, Caelifera) from China.

Science.gov (United States)

Gu, Jun-Jie; Yue, Yanli; Shi, Fuming; Tian, He; Ren, Dong

2016-09-20

Orthoptera is divided into two suborders, the Ensifera (katydids, crickets and mole crickets) and the Caelifera (grasshoppers and pygmy mole crickets). The earliest definitive caeliferans are those found in the Triassic (Bethoux & Ross 2005). The extinct caeliferan families, such as Locustopsidae and Locustavidae, may prove to be stem groups to some of the modern superfamilies (Grimaldi & Engel 2005). Locustopsidae is known from the Late Triassic or Early Jurassic to Late Cretaceous, consisting of two subfamilies (Gorochov et al. 2006). They are recorded from Europe, England, Russia, central Asia, China, Egypt, North America, Brazil and Australia. Up to now, Late Mesozoic fossil deposits of China has been reported plenty taxa of orthopterids, e.g. ensiferans, phasmatodeans, grylloblattids (Cui et al. 2012; Gu et al. 2010; Gu et al. 2012a; Gu et al. 2012b; Ren et al. 2012; Wang et al. 2014); but, with few caeliferans records, only four species, Pseudoacrida costata Lin 1982, Mesolocustopsis sinica Hong 1990, Tachacris stenosis Lin 1977 and T. turgis Lin 1980, were reported from the Early Cretaceous of Ningxia, Shandong, Yunnan and Zhejiang of China.

Diabetes Mellitus and Osteoporosis

Directory of Open Access Journals (Sweden)

Dilek Durmuş

2005-09-01

Full Text Available Osteoporosis is a condition of bone fragility resulting from micro-architectural deterioration and decreased bone mass. Studies on the presence of a generalized osteoporosis related to diabetes mellitus (DM are few and controversial. Factors associated with osteoporosis diabetes in which may account for the patogenesis of diabetic bone loss have been studied. This article will review the relevant litarature relating to diabetes and osteoporosis including cellular and animal models. These studies include vascular and neuropathic mechanism, poor glisemic control, abnormalities of calcium and vitamin D metabolism and hypercalciuria with secondary increase in parathyroid hormone secretion, the role of insülin and insülin like growth factor I. It appears that there is a great deal of variability in the bone mineral density and fracture rates in both type I and type II DM. This may reflect multiple factors such as the population, age, duration of diabetes and insülin use. There is need for further longitudinal studies, including the incidence and risk factors for osteoporosis in DM.

Violência e sociedade em Festa no Covil, de Juan Pablo Villalobos

Directory of Open Access Journals (Sweden)

Andre Rezende Benatti

2014-12-01

Full Text Available O presente artigo tem como objetivo uma leitura da violência no romance Festa no Covil, do escritor mexicano Juan Pablo Villalobos. Abordaremos dentre outras questões como a violência se torna parte da rotina do pequeno narrador do romance, Tochtli, tornando-se não mais uma surpresa ou estranheza ou repulsa, mas algo corriqueiro que o acompanha diariamente. No romance, Tochtli é o herdeiro de um cartel de narcotráfico e é criado em um meio no qual a violência está tão presente que o menino não a percebe. Para a leitura nos valeremos de conceitos de Ronaldo Lima Lins (1990 e José Ovejero (2012.

Fair trade - Õiglane kaubandus / Tuuliki Kasonen-Lins

Index Scriptorium Estoniae

Kasonen-Lins, Tuuliki

2007-01-01

Õiglase kaubanduse liikumise ajaloolisest arengust. Fair trade on kaubandusvõrk, kus kaubeldakse arengumaades inimväärsetes tingimustes toodetud ja sealsetelt kaupmeestelt õiglaste hindadega ostetud kaupadega. Fair Trade Euroopas

CSR kui äristrateegia osa / Tuuliki Kasonen-Lins

Index Scriptorium Estoniae

Kasonen-Lins, Tuuliki

2008-01-01

Autor selgitab, et ettevõtte ühiskondlik vastutus ei ole kord aastas korraldatav heategevuskampaania. Näitena ettevõtte ühiskondlikust vastutusest nimetab autor panustamist tööjõu koolitusse või töötute sotsiaalsete gruppidega tegelemisse

Ultrasonic measurement of gap between calandria tube and liquid injection shutdown system tube in PHWR

International Nuclear Information System (INIS)

Kim, Tae Ryong; Sohn, Seok Man; Lee, Jun Shin; Lee, Sun Ki; Lee, Jong Po

2001-01-01

Sag of CT or liquid injection shutdown system tubes in pressurized heavy water reactor is known to occur due to irradiation creep and growth during plant operation. When the sag of CT is big enough, the CT tube possibly comes in contact with liquid injection shutdown system tube (LIN) crossing beneath the CT, which subsequently may prevent the safe operation. It is therefore necessary to check the gap between the two tubes in order to confirm no contacts when using a proper measure periodically during the plant life. An ultrasonic gap measuring probe assembly which can be fed through viewing port installed on the calandria was developed and utilized to measure the sags of both tubes in a pressurized heavy water reactor in Korea. It was found that the centerlines of CT and LIN can be precisely detected by ultrasonic wave. The gaps between two tubes were easily obtained from the relative distance of the measured centerline elevations of the tubes. But the measured gap data observed at the viewing port were actually not the data at the crossing point of CT and LIN. To get the actual gap between two tubes, mathematical modeling for the deflection curves of two tubes was used. The sags of CT and LIN tubes were also obtained by comparison of the present centerlines with the initial elevations at the beginning of plant operation. The gaps between two tubes in the unmeasurable regions were calculated based on the measurement data and the channel power distribution

Clinical targeting recombinant immunotoxins for cancer therapy

Directory of Open Access Journals (Sweden)

Li M

2017-07-01

Full Text Available Meng Li,1,* Zeng-Shan Liu,1,* Xi-Lin Liu,1,* Qi Hui,2,* Shi-Ying Lu,1 Lin-Lin Qu,1 Yan-Song Li,1 Yu Zhou,1 Hong-Lin Ren,1 Pan Hu1 1Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, China-Japan Union Hospital, The First Hospital, Jilin University, Changchun, 2School of Pharmacy, Wenzhou Medical University, Wenzhou, People’s Republic of China *These authors contributed equally to this work Abstract: Recombinant immunotoxins (RITs are proteins that contain a toxin fused to an antibody or small molecules and are constructed by the genetic engineering technique. RITs can bind to and be internalized by cells and kill cancerous or non-cancerous cells by inhibiting protein synthesis. A wide variety of RITs have been tested against different cancers in cell culture, xenograft models, and human patients during the past several decades. RITs have shown activity in therapy of several kinds of cancers, but different levels of side effects, mainly related to vascular leak syndrome, were also observed in the treated patients. High immunogenicity of RITs limited their long-term or repeat applications in clinical cases. Recent advances in the design of immunotoxins, such as humanization of antibody fragment, PEGylation, and modification of human B- and T-cell epitopes, are overcoming the above mentioned problems, which predict the use of these immunotoxins as a potential therapeutic method to treat cancer patients. Keywords: targeted therapy, hematologic malignancies, solid tumors, vascular leak syndrome, immunogenicity 

Fuel cycle math - part one

International Nuclear Information System (INIS)

Anon.

1992-01-01

This article is Part One of a two-part article that reviews some of the numbers associated with the nuclear fuel cycle. The contents of Part One include: composition of the element uranium, considering atomic mass and weight-percent of the isotopes; uranium in the ground, including ore grades; mining, with dilution factors and recovery rates; ore sorting, including concentration factors; and uranium recovery. No financial information is presented in either Part One or Part Two

Vehicle parts detection based on Faster - RCNN with location constraints of vehicle parts feature point

Science.gov (United States)

Yang, Liqin; Sang, Nong; Gao, Changxin

2018-03-01

Vehicle parts detection plays an important role in public transportation safety and mobility. The detection of vehicle parts is to detect the position of each vehicle part. We propose a new approach by combining Faster RCNN and three level cascaded convolutional neural network (DCNN). The output of Faster RCNN is a series of bounding boxes with coordinate information, from which we can locate vehicle parts. DCNN can precisely predict feature point position, which is the center of vehicle part. We design an output strategy by combining these two results. There are two advantages for this. The quality of the bounding boxes are greatly improved, which means vehicle parts feature point position can be located more precise. Meanwhile we preserve the position relationship between vehicle parts and effectively improve the validity and reliability of the result. By using our algorithm, the performance of the vehicle parts detection improve obviously compared with Faster RCNN.

Thrombocytopenia responding to red blood cell transfusion

International Nuclear Information System (INIS)

Mubarak, Ahmad A.; Awidi, Abdalla; Rasul, Kakil I.; Al-Homsi, Ussama

2004-01-01

Three patients with severe symptomatic iron defficiency anemia and thrombocytopenia had a significant rise in the platelet count a few days following packed red blood cell transfusion. Pretransfusion platelet count of of patient one was 17x10/L. 22x10/Lin patient two and 29x10/L in patient three. On the 6th day of post tranfusion, the platelet count rose to 166x10/Lin patient one, 830x10/L in patient two and 136x10/L in patient three. The possible mechcnism behind such an unreported observation are discussed. (author)

Mississippi River: Study of Alternatives for Rehabilitation of Lock and Dam Number 1. Minneapolis, Minnesota. Volume 2. Appendix A. Alternative Plans of Rehabilitation.

Science.gov (United States)

1976-04-01

25,00 oo4 L.S I 1 00 L.S 15 000 L.t. 1 0SIOOO L.S. is 1 3,00l 2 00 ,o000 c.Y. 3.00 27 000 9.000 C.V. 3 27 000 9000 c .. 3.00 - . rZ 27000 N 00...34_ __ 75.00 _45( 6 ea. 75.00 ___ _ 5 6 ea. .0L0 _450 6 0g. .00.~ * 1.00 1s 180 lin.ft. 1.00 190 180 lin.ft. 1.00 ISO 1e0 180 1 .ft. 1.80 1" * 0.30 ___ _ 1 500

Are part timers real GPs? Attitudes of general practitioners toward those who work part time.

Science.gov (United States)

Mazza, D; Northfield, S

2000-09-01

Current attitudes within the medical profession suggest that one cannot be a 'real' doctor unless one works full time. The aim of this study was to determine the views and attitudes of a sample of GPs toward part time practice. As part of a larger study, GPs in two divisions in metropolitan Melbourne were sent a questionnaire based survey. GPs were asked to rate statements about part time practice using a Likert scale. The response rate was 55% (n = 246). The majority of GPs disagreed with the statement 'part time GPs are not real GPs' but feelings were mixed about whether part timers covered the same range of clinical content as full timers. One fifth of respondents questioned the competence of part time GPs. Male GPs, those over 55 years and full timers were more likely to hold negative views toward part time general practice. Our results show a 'generational' pattern toward part time practice. With the increasing numbers of women entering general practice, these attitudes will need to change to accommodate the fact that more GPs will be choosing to work part time in the future.

Cancer Health Disparities Research: Where have we been and where should we go?

Science.gov (United States)

Scarlett Lin Gomez, PhD, MPH, is Professor in the Department of Epidemiology and Biostatistics and a member of the Helen Diller Family Comprehensive Cancer Center at the University of California, San Francisco. She is also Director of the Greater Bay Area Cancer Registry, a part of the California Cancer Registry and the NCI Surveillance Epidemiology End Results (SEER) Program. Her research focuses primarily on cancer health disparities and aims to understand the multilevel drivers of those disparities. She has contributed surveillance data regarding cancer incidence and outcome patterns and trends for distinct Asian American, Native Hawaiian, and Pacific Islander and Hispanic ethnic groups, as well as cancer patterns by nativity status and neighborhood characteristics. She developed the California Neighborhoods Data System, a compilation of small-area level data on social and built environment characteristics, and has used these data in more than a dozen funded studies to evaluate the impact of social and built neighborhood environment factors on disease outcomes. Since 1996, Dr. Lin Gomez has received many honors and awards, including being named Author of the Year in 2010 by the American Journal of Public Health, the Above and Beyond Excellence Award in 2012 and the Mentoring Award in 2014, both by the Cancer Prevention Institute of California. She completed her education in epidemiology with an MPH at the University of Michigan, Ann Arbor, and her PhD at Stanford.

Pediatricians Working Part-Time Has Plateaued.

Science.gov (United States)

Cull, William L; Frintner, Mary Pat; O'Connor, Karen G; Olson, Lynn M

2016-04-01

To examine trends in pediatricians working part-time and residents seeking part-time work and to examine associated characteristics. The American Academy of Pediatrics (AAP) Periodic Survey of Fellows and the AAP Annual Survey of Graduating Residents were used to examine part-time employment. Fourteen periodic surveys were combined with an overall response rate of 57%. Part-time percentages were compared for surveys conducted from 2006-2009 and 2010-2013. The AAP Annual Surveys of Graduating Residents (combined response rate = 60%) from 2006-2009 were compared with 2010-2013 surveys for residents seeking and obtaining part-time positions following training. Multivariable logistic regression models identified characteristics associated with part-time work. Comparable percentages of pediatricians worked part-time in 2006-2009 (23%) and 2010-2013 (23%). There was similarly no statistically significant difference in residents seeking part-time work (30%-28%), and there was a slight decline in residents accepting part-time work (16%-13%, aOR .75, 95% CI .56-.96). Increases in working part-time were not found for any subgroups examined. Women consistently were more likely than men to work part-time (35% vs 9%), but they showed different patterns of part-time work across age. Women in their 40s (40%) were more likely than other women (33%) and men in their 60s (20%) were more likely than other men (5%) to work part-time. There has been a levelling off in the number of pediatricians working part-time and residents seeking part-time work. Overall, women remain more likely to work part-time, although 1 in 5 men over 60 work part-time. Copyright © 2016 Elsevier Inc. All rights reserved.

Transport properties of gaseous ions over a wide energy range, IV

International Nuclear Information System (INIS)

Viehland, L.A.; Mason, E.A.

1995-01-01

This paper updates three previous papers entitled open-quotes Transport Properties of Gaseous Ions over a Wide Energy Range.close quotes. These papers referred to as Parts I, II, and III, were by H.W.Ellis, P.Y. Pai, E.W. McDaniel, E.A. Mason, and L.A. Viehland, S.L. Lin, M.G. Thackston. Part IV contains compilations of experimental data on ionic mobilities and diffusion coefficients (both longitudinal and transverse) for ions in neutral gases in an externally applied electrostatic field, at various gas temperatures; the data are tabulated as a function of the ionic energy parameter E/N, where E is the electric field strength and N is the number density of the neutral gas. Part IV also contains a locator key to ionic mobilities and diffusion coefficients compiled in Parts I-IV. The coverage of the literature extends into 1994. The criteria for selection of the data are; (1) the measurements must cover a reasonably wide range of E/N; (2) the identity of the ions must be well established; and (3) the accuracy of the data must be good. 26 refs., 6 tabs

CINCO VOZES NUM COTIDIANO DE SOMBRAS: “PENTÁGONO DE HAHN”, DE OSMAN LINS

Directory of Open Access Journals (Sweden)

Cícero Émerson do Nascimento Cardoso (URCA

2013-11-01

Full Text Available Este trabalho se pauta na análise da obra “Pentágono de Hahn”, que integra a obra “Nove, novena – narrativas”, de Osman Lins. A partir da chegada de um circo a uma cidade interiorana que apresenta, dentre seus espetáculos, a elefanta “Hahn”, cinco personagens expõem suas existências fadadas à brutalidade de um cotidiano “repleto” de vazios e experiências não vividas. O autor, nessa obra, arquiteta uma enredística polifônica que excede em lirismo, acuidade psicológica e catalogação de valores humanos indevassáveis o que a torna um profícuo objeto de estudo aberto às mais amplas incursões em se tratando de produção literária moderna.

Palavras-chave: Modernismo; Narrativa moderna; Osman Lins.